"""Runtime of CHEM-163 instrument.biotek_405ts - Agilent BioTek 405 TS microplate washer, single 96-tube manifold, internal buffer switching (A-D).

Process model (SI; host clock only - step(dt_s) is the only way time advances):
  - fluid path: four supply bottles on the buffer valves A-D, 108 + 2 mL internal dead volume (guide p.38) as two stirred tanks in series - the
    dispensed liquid's purity after priming V is 1 - exp(-2V/V_dead)(1 + 2V/V_dead) (a 300 mL prime gives >= 95 %, the manufacturer recommendation); external vacuum
    pump on the switched accessory outlet, two waste bottles (the one with the level sensor stops a run when full), fluid / flow / vacuum / waste sensors
    (instrument configuration screen);
  - wash step (guide p.43-46): carrier to the manifold, per cycle aspirate (manifold descends at the travel rate to the aspirate height, Z steps x
    0.127 mm above the plate seat, delay, optional secondary aspirate) then dispense (volume 50-3000 uL/well at flow rates 3-11, cell-wash rates 1-2 only
    with the cell-wash option; vacuum delay; with the vacuum on, the aspirate tips at the dispense height skim the liquid above the tip (tip below the rim)
    or above the pinned meniscus (tip up to 1.5 mm above the rim); a blocked aspirate tube does not skim), optional
    bottom wash, soak and shake (carrier sent home when soak + shake exceed 1 min, vacuum pump off), final aspirate, carrier home;
  - wells (plate_library.json: volume, depth, SBS well-bottom elevation, mean radius; per-plate bottom-elevation scatter): residual volume after an
    aspiration = bottom layer A g^2/(g + g0) (g = tip height above the well bottom) + floor film A delta0 + wall film (Landau-Levich,
    t = 0.94 l_c Ca^(2/3), Ca = mu U / sigma, l_c = sqrt(sigma / (rho g)), wetted height V / A) + bottom-corner fillet, with the liquid density,
    viscosity and surface tension of wash_liquid_library.json at the room temperature; calibrated on the two manufacturer statements (<= 2 uL at the
    default 29 steps with water + 0.1 % Tween 20; about 100 uL at 50 steps);
  - wash efficiency: two-compartment wells - the dispense jet mixes the old liquid into the fresh buffer with the efficiency m_jet; the unmixed part
    (1 - m_jet) V0 / (V0 + v) stays as a bottom layer, which soak and shake mix in; the next aspiration leaves the bottom layer first; non-specifically adsorbed
    species desorb (k_off, faster with surfactant); specifically bound species stay (slow k_off); overflow when a well exceeds its volume (spill to the
    four neighbours: cross-contamination); drips from the parked manifold onto a plate left under it;
  - adherent cells: detachment by the dispense jet wall shear (jet speed from the tube flow), by aspiration close to the cell layer, and by aspiration
    to dryness with a long dwell; the cell-wash settings (rates 1-2 through the low-flow tubing, CW travel rates, high aspirate height) protect them;
  - tube clogging: dispense and aspirate tubes clog when salt / protein buffers dry in an idle manifold (rate by the salt mass fraction of the liquid
    left in the tubes and the idle time; a water rinse keeps the manifold wet); partially clogged dispense tubes under-dispense, clogged aspirate tubes
    leave their wells full; the flow sensor reports a total-flow deficit (Verify clog detection is a "Q"-model option: unsupported);
  - wash log (programme, cycles, alarms), waste and buffer bookkeeping, energy ledger; the plate content (unbound / non-specific / specific amounts,
    cells, residual volumes, clogs) is evaluator truth.
Generic schema / capability / session framework follows the CHEM-162 runtime.
"""
from __future__ import annotations

import copy
import functools
import hashlib
import json
import math
from pathlib import Path

import numpy as np

PROTOCOL_VERSION = "1.0.0"
ASSET_TYPE_ID = "instrument.biotek_405ts"
CATALOG_ID = "CHEM-163"
ROOT = Path(__file__).resolve().parents[2]
ASSET_DIR = ROOT / 'assets/expansion_batch04/t4_measure/biotek_405ts'
LIQUID_LIB_PATH = ASSET_DIR / 'wash_liquid_library.json'
PLATE_LIB_PATH = ASSET_DIR / 'plate_library.json'
G = 9.80665

# ---------------------------------------------------------------- manufacturer values (workbook row 168, 405 TS Getting Started Guide, EL406 manual)
VOLUME_RANGE_M3 = (50e-9, 3000e-9)          # 96-tube manifold dispense volume per well
VOLUME_STEP_M3 = 1e-9                       # 1 uL increments
CYCLES_RANGE = (1, 250)
Z_STEP_M = 0.127e-3                         # manifold height per Z step
ASP_Z_DEFAULT, DISP_Z_DEFAULT = 29, 121
ASP_DELAY_MAX_S = 5.0                       # 0-5000 ms
VAC_DELAY_M3 = (10e-9, 350e-9)
SHAKE_SOAK_MAX_S = 3600.0
HOME_AFTER_S = 60.0                         # carrier home when soak + shake exceed 1 min (vacuum pump off)
DEAD_VOLUME_M3 = 110e-6                     # 108 mL + 2 mL buffer-switching tubing
LINE_TANKS = 2                              # tanks-in-series of the fluid path (2: a 300 mL prime -> 97 %, 3 x dead volume -> 98 %; engineering, meets the >= 95 % statement)
PRIME_FOR_CHANGE_M3 = 300e-6                # manufacturer prime for a buffer change (>= 95 % purity)
MANIFOLD_PRIME_M3 = 50e-6
TRAVEL_SPEED = {'2': 3.4e-3, '3': 4.0e-3}   # m/s (EL406 manual, same manifold family)
ACCESSORY_OUTLET_A = 5.0
RESIDUAL_SPEC_M3 = 2e-9                     # <= 2 uL/well after a 3-cycle wash (0.1 % Tween 20 water, Costar flat)
CV_SPEC = 0.03                              # <= 3 % CV at 300 uL/well
# engineering values (not published)
FLOW_RATE_M3_S = {1: 10e-9, 2: 20e-9, 3: 25e-9, 4: 35e-9, 5: 50e-9, 6: 70e-9, 7: 100e-9, 8: 135e-9, 9: 175e-9, 10: 220e-9, 11: 270e-9}   # per well
TRAVEL_RATES = ['1', '2', '3', '4', '5', '1CW', '2CW', '3CW', '4CW', '6CW']
TRAVEL = {'1': (1.5e-3, 1.0), '2': (3.4e-3, 1.0), '3': (4.0e-3, 1.0), '4': (6.0e-3, 1.0), '5': (8.5e-3, 1.0),        # (speed m/s, disturbance factor)
          '1CW': (1.0e-3, 0.45), '2CW': (1.5e-3, 0.40), '3CW': (2.2e-3, 0.35), '4CW': (3.0e-3, 0.30), '6CW': (3.0e-3, 0.20)}
PARK_TIP_M = 0.034                          # aspirate tips above the plate seat when parked (scene joint 0; above the 250-step maximum 31.75 mm)
DISP_TIP_ABOVE_ASP_M = 0.0025
CARRIER_TRAVEL_M = 0.160                    # home (load) -> under the manifold (scene joint -0.160 m)
CARRIER_MOVE_S = 1.0
LIFT_SPEED_M_S = 0.030
VALVE_S = 0.4
TUBE_INNER_R_M = 0.25e-3                    # dispense tube bore
LOW_FLOW_R_M = 0.40e-3                      # cell-wash low-flow outlet (wider, slower jet)
G0_M = 0.10e-3                              # bottom-layer drainage length (50 steps -> ~88 uL in a Costar 360 uL well; guide: 'approximately 100 uL')
DELTA0_M = 20e-6                            # floor film left around the tip at the well bottom
FILLET_FACTOR = 0.10                        # bottom-corner fillet radius / capillary length
RESIDUAL_SCATTER = 0.12                     # lognormal scatter of the residual per well
WBE_SCATTER_M = 0.04e-3                     # well-bottom elevation scatter within a plate (SLAS-6 variation)
CV_REPEAT, CV_TUBE, CV_ABS_M3 = 0.012, 0.012, 1.0e-9    # dispense scatter: shot-to-shot, fixed tube-to-tube, absolute
MIX_JET_M_S = 0.6                           # jet speed for e-fold mixing of the residual into the fresh buffer
MIX_MIN = 0.5                               # convective mixing of the residual by a slowly poured buffer (low-flow dispense)
SHAKE_TAU = {'slow': 20.0, 'medium': 8.0, 'fast': 3.0, 'variable': 6.0}
SOAK_TAU_S = 1.0e4                          # soak: diffusive mixing of the residual layer (r^2 / D for small molecules)
K_OFF_NS, K_OFF_NS_SURF, K_OFF_SPEC = 0.004, 0.04, 2e-6  # 1/s: non-specific desorption (plain / with surfactant), specific
TAU_CELL_PA = 2.5                           # wall shear for e-fold detachment of adherent cells (weak adhesion; x4 strong)
CELL_LAYER_CLEAR_M = 0.35e-3                # tip gap below which aspiration scrapes the cell layer
DRY_DWELL_S = 20.0                          # a nearly dry well (< 3 uL) longer than this damages the monolayer
CLOG_TAU_S = 4.0 * 86400.0                  # idle-manifold clogging time scale for 1 % salt (scales with 1 / salt fraction)
MENISCUS_M = 1.5e-3                         # liquid pinned at the rim can bulge this far above it before it spills (engineering)
IDLE_W, ACTIVE_W = 14.0, 46.0               # electrical power (two internal 60 W supplies)
DROP_M3 = 0.25e-9                           # liquid on the outside of a tube tip after an aspiration
DROPS_PER_TIP = 4                           # drops a wetted dispense tube tip can shed before it is dry (engineering assumption)
DRIP_P_PER_S = 1.5e-4                       # per tube and second while a plate sits under the parked manifold


class ActionRejected(ValueError):
    """A capability call refused with a declared error code (no state change)."""


# ---------------------------------------------------------------- schema helpers
def number(unit='1', lo=None, hi=None):
    s = {'type': 'number', 'x-unit': unit}
    if lo is not None:
        s['minimum'] = lo
    if hi is not None:
        s['maximum'] = hi
    return s


def obj(properties, optional=()):
    return dict(type='object', properties=properties, required=[k for k in properties if k not in optional], additionalProperties=False)


def nullable(s):
    return {'anyOf': [s, {'type': 'null'}]}


def array(s, minimum=0, maximum=4096):
    return dict(type='array', items=s, minItems=minimum, maxItems=maximum)


def enum(items):
    return {'type': 'string', 'enum': list(items)}


BOOL = {'type': 'boolean'}
FREEOBJ = {'type': 'object'}                       # free-form mapping (settings echo, composition by liquid id)
TEXT = {'type': 'string'}
INT = {'type': 'integer', 'x-unit': '1'}


def integer(lo, hi):
    return {'type': 'integer', 'x-unit': '1', 'minimum': lo, 'maximum': hi}


def validate(s, v):
    if 'anyOf' in s:
        for o in s['anyOf']:
            try:
                validate(o, v)
                return
            except ActionRejected:
                pass
        raise ActionRejected('INVALID_ARGUMENT')
    k = s.get('type')
    if k in ('number', 'integer'):
        if isinstance(v, bool) or not isinstance(v, (int, float)) or not math.isfinite(v) or (k == 'integer' and not float(v).is_integer()):
            raise ActionRejected('INVALID_ARGUMENT')
        if not s.get('minimum', -math.inf) <= v <= s.get('maximum', math.inf):
            raise ActionRejected('INVALID_ARGUMENT')
    elif k == 'object':
        if not isinstance(v, dict):
            raise ActionRejected('INVALID_ARGUMENT')
        if 'properties' in s:
            req = set(s.get('required', s['properties']))
            if not req <= set(v) or not set(v) <= set(s['properties']):
                raise ActionRejected('INVALID_ARGUMENT')
            for key, value in v.items():
                validate(s['properties'][key], value)
    elif k == 'array':
        if not isinstance(v, list) or not s.get('minItems', 0) <= len(v) <= s.get('maxItems', math.inf):
            raise ActionRejected('INVALID_ARGUMENT')
        for x in v:
            validate(s['items'], x)
    elif k == 'boolean' and type(v) is not bool:
        raise ActionRejected('INVALID_ARGUMENT')
    elif k == 'string':
        if not isinstance(v, str) or len(v) > s.get('maxLength', 10 ** 6) or len(v) < s.get('minLength', 0):
            raise ActionRejected('INVALID_ARGUMENT')
        if 'pattern' in s:
            import re
            if not re.fullmatch(s['pattern'], v):
                raise ActionRejected('INVALID_ARGUMENT')
    elif k == 'null' and v is not None:
        raise ActionRejected('INVALID_ARGUMENT')
    if 'enum' in s and v not in s['enum']:
        raise ActionRejected('INVALID_ARGUMENT')


# ---------------------------------------------------------------- template libraries
@functools.lru_cache(maxsize=1)
def liquid_lib():
    return json.loads(LIQUID_LIB_PATH.read_text())


@functools.lru_cache(maxsize=1)
def plate_lib():
    return json.loads(PLATE_LIB_PATH.read_text())


def liquid_props(lid, T):
    """(density kg/m3, viscosity Pa s, surface tension N/m) of a library liquid at T (linear in T, log-linear for the viscosity), clamped to its grid."""
    e = liquid_lib()['substances'][lid]
    Ts = np.array(e['temperature_K'])
    t = float(np.clip(T, Ts[0], Ts[-1]))
    rho = float(np.interp(t, Ts, e['density_kg_m3']))
    mu = float(np.exp(np.interp(t, Ts, np.log(e['viscosity_Pa_s']))))
    sig = float(np.interp(t, Ts, e['surface_tension_N_m']))
    return rho, mu, sig


def mixture_props(comp, T):
    """Properties of a mixture of library liquids by volume fractions: linear density and surface tension, log-mean viscosity; the surface tension
    of a mixture with a surfactant-containing fraction >= 5 % takes the surfactant value (surface excess)."""
    if not comp:
        return liquid_props('water', T)
    tot = sum(comp.values())
    rho = mu_ln = sig = 0.0
    surf = [k for k in comp if liquid_lib()['substances'][k].get('surfactant')]
    for k, f in comp.items():
        r, m, s = liquid_props(k, T)
        rho += f / tot * r
        mu_ln += f / tot * math.log(m)
        sig += f / tot * s
    if surf and sum(comp[k] for k in surf) / tot >= 0.05:
        sig = min(liquid_props(k, T)[2] for k in surf)
    return rho, math.exp(mu_ln), sig


def salt_fraction(comp):
    """Mass fraction of dissolved salts of a mixture (drives drying / clogging of the tubes)."""
    tot = sum(comp.values()) or 1.0
    return sum(f / tot * sum(liquid_lib()['substances'][k].get('mass_fractions', {}).values()) for k, f in comp.items())


def is_surfactant(comp):
    tot = sum(comp.values()) or 1.0
    return sum(f for k, f in comp.items() if liquid_lib()['substances'][k].get('surfactant')) / tot >= 0.05


# ---------------------------------------------------------------- well physics
def film_volume(V, A, r, rho, mu, sig, U):
    """Landau-Levich wall film left by a receding meniscus at speed U on the wetted height V / A of a cylindrical well of radius r."""
    lc = math.sqrt(sig / (rho * G))
    Ca = mu * max(U, 1e-5) / sig
    t = 0.94 * lc * Ca ** (2.0 / 3.0)
    return 2 * math.pi * r * min(V / A, 0.02) * t


def residual_volume(V, gap, plate, rho, mu, sig, U, delay_s=0.0):
    """Liquid left in a well after aspiration with the tips gap m above the well bottom (gap <= 0: tips on the bottom)."""
    r = plate['mean_well_radius_m']
    A = math.pi * r * r
    g = max(0.0, gap)
    layer = A * g * g / (g + G0_M)
    floor = A * DELTA0_M
    lc = math.sqrt(sig / (rho * G))
    rf = FILLET_FACTOR * lc
    fillet = 2 * math.pi * r * (1 - math.pi / 4) * rf * rf
    film = film_volume(V, A, r, rho, mu, sig, U)
    if delay_s > 0 and film > 0:   # the aspirate delay lets the wall film drain to the bottom, where the tips take it: t = t0 / sqrt(1 + t/tau)
        H = min(V / A, 0.02)
        t0 = film / (2 * math.pi * r * max(H, 1e-6))
        film /= math.sqrt(1 + delay_s * rho * G * t0 ** 2 / (mu * max(H, 1e-6)))
    return min(V, layer + floor + fillet + film)


def jet_speed(rate):
    Q = FLOW_RATE_M3_S[rate]
    rr = LOW_FLOW_R_M if rate <= 2 else TUBE_INNER_R_M
    return Q / (math.pi * rr * rr)


def wall_shear(rate, depth, rho):
    """Wall shear stress of the dispense jet at the well bottom (Pa): stagnation pressure 0.5 rho v^2 scaled down with the liquid depth above the
    bottom (the jet decays over ~2 mm of liquid) and the tube offset to the side of the well (engineering)."""
    v = jet_speed(rate)
    return 0.5 * rho * v * v * 0.02 * math.exp(-depth / 2.0e-3)


# ---------------------------------------------------------------- capabilities and state slots
CAPS = {}


def cap(k, s, o, e, c, meaning, visibility='agent', kind='device_command'):
    CAPS[k] = dict(input_schema=s, output_schema=o, example_arguments=e, component_ids=c, function_zh=meaning, visibility=visibility, kind=kind)


BOTTLES = ['A', 'B', 'C', 'D']
SID = dict(type='string', minLength=1, maxLength=40, pattern='^[A-Za-z0-9_.-]+$')
LIBID = dict(type='string', minLength=1, maxLength=60)
PLANT_EXAMPLE = dict(room_k=295.15, mains_voltage_v=230.0, noise_seed=1, vacuum_pump=True, cell_wash_installed=False, waste_capacity_m3=0.008)
PLANT = obj(dict(room_k=number('K', 283.15, 313.15), mains_voltage_v=number('V', 0.0, 264.0), noise_seed=integer(0, 2 ** 31 - 1), vacuum_pump=BOOL,
                 cell_wash_installed=BOOL, waste_capacity_m3=number('m3', 0.004, 0.020)))
PLATE = obj(dict(plate_id=SID, plate_type=LIBID, fill_volume_m3=number('m3', 0.0, 3.6e-7), liquid_id=LIBID, unbound_mol=number('mol', 0.0, 1e-6),
                 nonspecific_mol=number('mol', 0.0, 1e-9), specific_mol=number('mol', 0.0, 1e-9), cells_per_well=number('1', 0.0, 2e5),
                 cell_adhesion=enum(['none', 'weak', 'strong']), wells=nullable(array(integer(0, 95), 1, 96))))
PLATE_EXAMPLE = dict(plate_id='elisa_plate', plate_type='costar_3590', fill_volume_m3=1.0e-7, liquid_id='pbs_1x', unbound_mol=1e-12, nonspecific_mol=2e-14,
                     specific_mol=5e-14, cells_per_well=0.0, cell_adhesion='none', wells=None)
TRAVEL_E = enum(TRAVEL_RATES)
WASH = obj(dict(buffer=enum(BOTTLES), cycles=integer(*CYCLES_RANGE), dispense_volume_m3=number('m3', *VOLUME_RANGE_M3), dispense_rate=integer(1, 11),
                dispense_z_steps=integer(40, 250), aspirate_z_steps=integer(1, 250), travel_rate=TRAVEL_E, aspirate_delay_s=number('s', 0.0, ASP_DELAY_MAX_S),
                secondary_aspirate_z_steps=nullable(integer(1, 250)), vacuum_delay_m3=number('m3', *VAC_DELAY_M3), soak_s=number('s', 0.0, SHAKE_SOAK_MAX_S),
                shake_s=number('s', 0.0, SHAKE_SOAK_MAX_S), shake_intensity=enum(list(SHAKE_TAU)), bottom_wash=BOOL, final_aspirate=BOOL))
WASH_EXAMPLE = dict(buffer='A', cycles=3, dispense_volume_m3=300e-9, dispense_rate=7, dispense_z_steps=DISP_Z_DEFAULT, aspirate_z_steps=ASP_Z_DEFAULT, travel_rate='3',
                    aspirate_delay_s=0.0, secondary_aspirate_z_steps=None, vacuum_delay_m3=10e-9, soak_s=0.0, shake_s=0.0, shake_intensity='medium', bottom_wash=False,
                    final_aspirate=True)
OPERATION = obj(dict(operation_id=TEXT))
STATUS = enum(['running', 'completed', 'failed', 'cancelled'])
ALARMS = ['WASTE_FULL', 'VACUUM_LOW', 'BUFFER_EMPTY', 'FLOW_ERROR', 'POWER_LOST']
RECORD = obj(dict(record_id=TEXT, kind=enum(['wash', 'prime', 'clean']), status=STATUS, started_s=number('s'), finished_s=nullable(number('s')), plate_id=nullable(TEXT),
                  settings=FREEOBJ, cycles_done=INT, programmed_dispense_m3=number('m3'), alarms=array(enum(ALARMS), 0, 12),
                  warnings=array(TEXT, 0, 12), template_source=obj(dict(liquids=array(TEXT, 0, 8), plate=nullable(TEXT)))))
EVENT = obj(dict(time_s=number('s'), kind=enum(['power', 'fluid', 'plate', 'program', 'alarm', 'fault', 'maintenance']), code=TEXT, message_zh=TEXT))
LEDGER_KEYS = ['electrical_energy_j', 'dispensed_m3', 'aspirated_m3', 'primed_m3', 'dripped_m3', 'waste_added_m3', 'overflow_m3', 'carrier_travel_m', 'manifold_travel_m', 'cycles']
LEDGER = obj({k: number('J' if k.endswith('_j') else 'm3' if k.endswith('_m3') else 'm' if k.endswith('_m') else '1') for k in LEDGER_KEYS})

cap('plant.configure', PLANT, obj(dict(plant=PLANT)), PLANT_EXAMPLE, ['frame_base', 'rear_connectors'],
    '宿主声明实验室条件与外部附件：室温、电源电压、噪声种子、真空泵是否接在附件插座、是否装细胞洗涤选件、废液瓶总容量', 'evaluator', 'simulation')
cap('power.set', obj(dict(enabled=BOOL)), obj(dict(enabled=BOOL)), dict(enabled=True), ['switch_power', 'rear_panel'], '主电源开/关')
cap('power.press_switch', obj({}), obj(dict(enabled=BOOL)), {}, ['switch_power'], '按一下后面板主电源开关（翻转开/关）')
cap('buffer.load', obj(dict(bottle=enum(BOTTLES), liquid_id=LIBID, volume_m3=number('m3', 0.0, 0.010))), obj(dict(bottle=TEXT, liquid_id=TEXT, volume_m3=number('m3'))),
    dict(bottle='A', liquid_id='pbst_0_05pct_tween20', volume_m3=0.004), ['buffer_inlets'], '向供液瓶A–D加注或更换洗液（液体取洗液模板库；瓶内原液体被替换）', 'agent', 'robot_skill')
cap('waste.empty', obj({}), obj(dict(emptied_m3=number('m3'))), {}, ['rear_connectors'], '倒空废液瓶（液位传感器复位）', 'agent', 'robot_skill')
cap('plate.provide', PLATE, obj(dict(plate_id=TEXT, location=TEXT)), PLATE_EXAMPLE, ['plate_carrier'],
    '在实验台上准备一块微孔板：板型取板模板库（96孔），各孔的初始液体、可溶游离物、非特异吸附物、特异结合物与贴壁细胞（真值只对评测者可见）', 'agent', 'robot_skill')
cap('plate.load', obj(dict(plate_id=SID)), obj(dict(plate_id=TEXT)), dict(plate_id='elisa_plate'), ['plate_carrier'],
    '把微孔板放到载板上（A1在左后角；载板在初始位、洗头停放、无运行）', 'agent', 'robot_skill')
cap('plate.unload', obj(dict(plate_id=SID)), obj(dict(plate_id=TEXT)), dict(plate_id='elisa_plate'), ['plate_carrier'], '从载板取下微孔板放回实验台', 'agent', 'robot_skill')
cap('prime.run', obj(dict(buffer=enum(BOTTLES), volume_m3=number('m3', 5e-6, 0.5e-3), rate=integer(3, 11))), OPERATION, dict(buffer='A', volume_m3=300e-6, rate=9),
    ['manifold', 'prime_trough', 'buffer_inlets'], '灌注：切换到所选缓冲阀，洗头停在灌注槽上方分配给定体积（换液推荐300 mL，洗头灌注50 mL），更新管路内液体组成', 'agent')
cap('wash.run', obj(dict(protocol=WASH)), OPERATION, dict(protocol=WASH_EXAMPLE), ['manifold', 'plate_carrier', 'buffer_inlets', 'touch_screen'],
    '运行洗板步骤：载板移到洗头下，逐循环抽吸（行进速度、抽吸高度、延时、二次抽吸）与分配（体积、流速、分配高度、真空延迟），可选底洗、浸泡、摇动与最终抽吸；结束回到初始位', 'agent')
cap('maintenance.clean_tubes', obj({}), OPERATION, {}, ['manifold', 'manifold_tubes'], '用清洁针逐根疏通抽吸/分配管（人工/机器人维护，约10 min；解除堵针）', 'agent', 'robot_skill')
cap('log.read', obj({}), obj(dict(entries=array(obj(dict(record_id=TEXT, kind=TEXT, status=TEXT, cycles_done=INT, alarms=array(TEXT, 0, 12), finished_s=nullable(number('s')))), 0, 200))),
    {}, ['touch_screen'], '读清洗日志（程序、完成循环、报警）', 'agent', 'query')
cap('measurement.result', obj(dict(record_id=TEXT)), RECORD, dict(record_id='WS0001'), ['touch_screen'], '读取一条运行记录（设置、完成循环、程序分配体积、报警、警告；实际分配/抽吸体积仪器不测）', 'agent', 'query')
cap('records.list', obj({}), obj(dict(records=array(TEXT, 0, 200))), {}, ['touch_screen'], '列出记录', 'agent', 'query')
cap('events.read', obj(dict(since_s=number('s', 0.0))), obj(dict(events=array(EVENT, 0, 400), dropped=INT)), dict(since_s=0.0), ['frame_base'], '读事件日志', 'agent', 'query')
cap('ledger.read', obj({}), obj(dict(ledger=LEDGER)), {}, ['frame_base'], '读守恒账目（电能、分配/抽吸/灌注/废液/溢流体积、行程、循环数）', 'evaluator', 'query')
cap('plate.inspect', obj(dict(plate_id=SID)), obj(dict(plate_id=TEXT, volume_m3=array(number('m3'), 96, 96), unbound_mol=array(number('mol'), 96, 96),
                                                    nonspecific_mol=array(number('mol'), 96, 96), specific_mol=array(number('mol'), 96, 96), cells=array(number('1'), 96, 96),
                                                    contamination_mol=array(number('mol'), 96, 96))),
    dict(plate_id='elisa_plate'), ['plate_carrier'], '评测者读板内真值：各孔液体体积、游离物、非特异/特异结合物、细胞数与交叉污染量', 'evaluator', 'query')
cap('provenance.read', obj({}), obj(dict(libraries=array(obj(dict(path=TEXT, sha256=TEXT, count=INT)), 2, 2))), {}, ['frame_base'], '模板库路径与sha256', 'agent', 'query')
cap('fault.inject', obj(dict(fault=enum(['vacuum_leak', 'buffer_line_air', 'dispense_tube_clog', 'aspirate_tube_clog', 'waste_sensor_stuck', 'power_loss']),
                             tube=nullable(integer(0, 95)), active=BOOL)), obj(dict(faults=array(TEXT, 0, 8))), dict(fault='dispense_tube_clog', tube=5, active=True),
    ['frame_base', 'manifold_tubes'], '评测者故障注入：真空泄漏、缓冲管进气、指定管堵塞（分配/抽吸）、废液传感器卡住、断电', 'evaluator', 'simulation')
cap('sim.step', obj(dict(dt_s=number('s', 0.0, 86400.0))), obj(dict(time_s=number('s'))), dict(dt_s=1.0), ['frame_base'], '推进宿主时钟（空闲时间让管路干涸）', 'evaluator', 'simulation')
UNSUPPORTED = {
    'vacuum_filtration.run': '真空过滤（滤板底部抽滤）为选件（Vac配置、止动螺钉套件），本配置未装',
    'magnetic_bead.wash': '磁珠分离洗涤需磁板与止动螺钉套件，本配置未装',
    'verify.clog_test': 'Verify堵针检测只在“Q”型号上提供，本机不支持（堵针只能由流量传感器的总流量偏差间接提示）',
    'ultrasonic.autoclean': '超声自动清洗（AutoClean）为选件',
    'biostack.run': 'BioStack堆叠机联机未建模',
    'hardware.connect': '没有真实405 TS与LHC软件的硬件接口',
}
for _k, _why in UNSUPPORTED.items():
    cap(_k, obj({}), obj({}), {}, ['frame_base'], _why, 'agent', 'device_command')

SLOTS = {}


def slot(k, schema, unit, origin, component, meaning, visibility='agent', reference=None):
    SLOTS[k] = dict(schema=schema, unit=unit, origin=origin, component=component, meaning=meaning, visibility=visibility, reference=reference)


slot('clock.time_s', number('s'), 's', 'derived', 'frame_base', '仿真时钟')
slot('plant.configured', BOOL, '1', 'setpoint', 'frame_base', '已声明实验室条件')
slot('power.enabled', BOOL, '1', 'setpoint', 'switch_power', '主电源')
slot('carrier.x_m', number('m'), 'm', 'derived', 'plate_carrier', '载板位置（0为初始装板位，−0.160 m为洗头下；场景载板关节镜像）',
     reference='资产坐标+X方向的平移，0=初始位（板中心x=0.078 m），−0.160 m=洗头下（板中心x=−0.082 m）')
slot('carrier.at_home', BOOL, '1', 'derived', 'plate_carrier', '载板在初始位（可装卸板）')
slot('manifold.z_m', number('m'), 'm', 'derived', 'manifold', '洗头位置（0为停放，管尖高于板位34 mm；场景洗头关节镜像）',
     reference='资产坐标+Z方向的平移，抽吸管尖高于载板板位 = 0.034 m + 该值；Z步长0.127 mm')
slot('manifold.tip_height_m', number('m'), 'm', 'derived', 'manifold', '抽吸管尖高于载板板位的高度', reference='载板板位（板底）为零点')
slot('buffer.selected', enum(BOTTLES), '1', 'setpoint', 'buffer_inlets', '当前缓冲阀')
for _b in BOTTLES:
    slot(f'buffer.{_b.lower()}.liquid', nullable(TEXT), '1', 'setpoint', 'buffer_inlets', f'供液瓶{_b}中的洗液（模板库ID）')
    slot(f'buffer.{_b.lower()}.volume_m3', number('m3'), 'm3', 'process_truth', 'buffer_inlets', f'供液瓶{_b}中的液体体积（宿主记账真值；仪器只有液体传感器）', 'evaluator', reference='液体实际体积（室温）')
slot('sensor.fluid_ok', BOOL, '1', 'sensor', 'buffer_inlets', '液体传感器：所选供液瓶有液')
slot('sensor.vacuum_ok', BOOL, '1', 'sensor', 'rear_connectors', '真空传感器：真空泵接好且无泄漏（抽吸时）')
slot('sensor.waste_full', BOOL, '1', 'sensor', 'rear_connectors', '废液瓶液位传感器：满')
slot('sensor.flow_ok', BOOL, '1', 'sensor', 'manifold', '流量传感器：上次分配总流量与设定相符（偏差<10 %）')
slot('program.phase', nullable(TEXT), '1', 'derived', 'touch_screen', '运行中的阶段（移动/抽吸/分配/浸泡/摇动/灌注/清洁）')
slot('operation.kind', nullable(TEXT), '1', 'derived', 'touch_screen', '进行中的操作（wash/prime/clean）')
slot('operation.progress', number('1'), '1', 'derived', 'touch_screen', '操作进度0–1')
slot('operation.started_by_other_agent', BOOL, '1', 'derived', 'touch_screen', '进行中的操作属于另一个agent')
slot('display.cycles_remaining', INT, '1', 'derived', 'display_cycles_digits', '屏幕仿真数字：剩余循环数（无运行时0）')
slot('display.time_remaining_s', number('s'), 's', 'derived', 'display_time_digits', '屏幕仿真数字：剩余时间（无运行时0）')
slot('result.last_record_id', nullable(TEXT), '1', 'derived', 'touch_screen', '最近完成的记录')
slot('plate.on_carrier', nullable(TEXT), '1', 'derived', 'plate_carrier', '载板上的微孔板')
slot('plate.bench_ids', array(TEXT, 0, 20), '1', 'derived', 'plate_carrier', '实验台上的板')
slot('truth.line_composition', FREEOBJ, '1', 'process_truth', 'buffer_inlets', '管路（死体积110 mL）内液体按体积分数的组成', 'evaluator')
slot('truth.waste_volume_m3', number('m3'), 'm3', 'process_truth', 'rear_connectors', '废液瓶中液体体积', 'evaluator', reference='液体实际体积（室温）')
slot('truth.residual_m3', nullable(array(number('m3'), 96, 96)), 'm3', 'process_truth', 'plate_carrier', '载板上板各孔的液体体积（A1..H12按列优先）', 'evaluator',
     reference='孔序：列1的A–H、列2的A–H……（index = 8 x 列 + 行）')
slot('truth.clogged_dispense', array(integer(0, 95), 0, 96), '1', 'process_truth', 'manifold_tubes', '堵塞（部分或全部）的分配管序号', 'evaluator')
slot('truth.clogged_aspirate', array(integer(0, 95), 0, 96), '1', 'process_truth', 'manifold_tubes', '堵塞的抽吸管序号', 'evaluator')
slot('truth.faults', array(TEXT, 0, 8), '1', 'process_truth', 'frame_base', '注入的故障', 'evaluator')
slot('truth.idle_s', number('s'), 's', 'process_truth', 'manifold_tubes', '洗头自上次用水冲洗或运行以来的空闲时间', 'evaluator')
MAX_RECORDS, MAX_EVENTS, MAX_PLATES = 200, 400, 20


class _Sum:
    def __init__(self):
        self.s = 0.0
        self.c = 0.0

    def add(self, x):
        t = self.s + x
        if abs(self.s) >= abs(x):
            self.c += (self.s - t) + x
        else:
            self.c += (x - t) + self.s
        self.s = t

    def value(self):
        return self.s + self.c


class Biotek405tsRuntime:
    """405 TS microplate washer: power, buffer bottles and valves, prime, wash step (aspirate / dispense / soak / shake), plate carrier and manifold
    positions, sensors, wash log; plates with per-well liquid, soluble / adsorbed / bound species and adherent cells (SI units)."""

    def __init__(self, instance_id='washer_01'):
        self.instance_id = instance_id
        self.t = 0.0
        self.revision = 0
        self.plant = None
        self.power = False
        self.bottles = {b: dict(liquid=None, volume=0.0) for b in BOTTLES}
        self.valve = 'A'
        self.tanks = [{'water': 1.0} for _ in range(LINE_TANKS)]   # the fluid path (tanks in series) is wet with water (primed at installation)
        self.waste = 0.0
        self.carrier = 0.0
        self.manifold = 0.0
        self.phase = None
        self.plates = {}
        self.on_carrier = None
        self.records = {}
        self.seq = dict(ws=0, pr=0, cl=0, op=0)
        self.op = None
        self.operations = {}
        self.faults = set()
        self.clog_d = {}                                   # tube -> remaining flow fraction (0 = blocked)
        self.clog_a = set()
        self.idle_s = 0.0
        self.idle_n, self.idle_H, self.clog_E = 0, 0.0, None   # idle clogging: hazard integral and pre-sampled per-tube thresholds
        self.drip_clock, self.drip_next = 0.0, None        # plate-under-manifold exposure time and each tube's next drop
        self.drip_left = np.zeros(96, int)                 # drops still hanging at each tube tip (refilled by a dispense / prime)
        self.tip_liquid = {'water': 1.0}                   # composition of the liquid wetting the tube tips
        self.flow_ok = True
        self.events, self.events_dropped = [], 0
        self.led = {k: _Sum() for k in LEDGER_KEYS}
        self._tube_bias = None
        self._principals, self._requests, self._owners = {}, {}, {}

    # ---------------------------------------------------------------- basics
    @property
    def time_s(self):
        return self.t

    def register_principal(self, name, visibility):
        if visibility not in ('agent', 'evaluator') or name in self._principals:
            raise ActionRejected('INVALID_ARGUMENT')
        self._principals[name] = visibility

    def session(self, name):
        if name not in self._principals:
            raise ActionRejected('ACCESS_DENIED')
        return Biotek405tsSession(self, name)

    def _event(self, kind, code, message, rid=None):
        if len(self.events) >= MAX_EVENTS:
            self.events.pop(0)
            self.events_dropped += 1
        self.events.append(dict(time_s=self.t, kind=kind, code=code, message_zh=message, _rid=rid))

    def _rng(self, tag):
        h = hashlib.sha256(f'{self.plant["noise_seed"] if self.plant else 0}:{tag}'.encode()).digest()
        return np.random.default_rng(int.from_bytes(h[:8], 'little'))

    def _T(self):
        return self.plant['room_k'] if self.plant else 295.15

    def _vacuum_ok(self):
        return bool(self.plant and self.plant['vacuum_pump'] and self.power and 'vacuum_leak' not in self.faults)

    def _waste_full(self):
        if 'waste_sensor_stuck' in self.faults:
            return True
        return bool(self.plant) and bool(self.waste >= 0.9 * self.plant['waste_capacity_m3'])

    def _fluid_ok(self, b=None):
        b = b or self.valve
        return bool(self.bottles[b]['volume'] > 1e-6 and self.bottles[b]['liquid'] is not None and 'buffer_line_air' not in self.faults)

    def _tip(self):
        return PARK_TIP_M + self.manifold

    def _remaining(self):
        o = self.op
        if o is None:
            return 0, 0.0
        left = [p for p in o['phases'][o['i']:]]
        cycles = sum(1 for p in left if p['name'] == 'dispense')
        t_left = max(0.0, o['t_end'] - self.t)
        return cycles, t_left

    def read(self, visibility='agent'):
        o = self.op
        prog = 0.0 if o is None else min(1.0, (self.t - o['t0']) / max(1e-9, o['t_end'] - o['t0']))
        cyc, tl = self._remaining()
        p = self.plates.get(self.on_carrier)
        values = {
            'clock.time_s': self.t, 'plant.configured': self.plant is not None, 'power.enabled': self.power, 'carrier.x_m': self.carrier,
            'carrier.at_home': abs(self.carrier) < 1e-12, 'manifold.z_m': self.manifold, 'manifold.tip_height_m': self._tip(), 'buffer.selected': self.valve,
            **{f'buffer.{b.lower()}.liquid': self.bottles[b]['liquid'] for b in BOTTLES}, **{f'buffer.{b.lower()}.volume_m3': self.bottles[b]['volume'] for b in BOTTLES},
            'sensor.fluid_ok': self._fluid_ok(), 'sensor.vacuum_ok': self._vacuum_ok(), 'sensor.waste_full': self._waste_full(), 'sensor.flow_ok': self.flow_ok,
            'program.phase': self.phase, 'operation.kind': o['kind'] if o else None, 'operation.progress': prog, 'operation.started_by_other_agent': False,
            'display.cycles_remaining': int(cyc) if self.power else 0, 'display.time_remaining_s': float(round(tl)) if self.power else 0.0,
            'result.last_record_id': self._last_record(), 'plate.on_carrier': self.on_carrier,
            'plate.bench_ids': sorted(k for k, v in self.plates.items() if v['location'] == 'bench'),
            'truth.line_composition': dict(self.line), 'truth.waste_volume_m3': self.waste,
            'truth.residual_m3': None if p is None else [float(v) for v in p['V']], 'truth.clogged_dispense': sorted(self.clog_d),
            'truth.clogged_aspirate': sorted(self.clog_a), 'truth.faults': sorted(self.faults), 'truth.idle_s': self.idle_s,
        }
        return {k: v for k, v in values.items() if SLOTS[k]['visibility'] == 'agent' or visibility == 'evaluator'}

    def _last_record(self, keep=None):
        done = [k for k, r in self.records.items() if r['status'] == 'completed' and (keep is None or keep(k))]
        return max(done, key=lambda k: (self.records[k]['finished_s'] or 0.0, k), default=None)

    # ---------------------------------------------------------------- commands
    def invoke(self, capability_id, **a):
        if capability_id not in CAPS:
            raise ActionRejected('UNKNOWN_CAPABILITY')
        if capability_id in UNSUPPORTED:
            raise ActionRejected('UNSUPPORTED')
        validate(CAPS[capability_id]['input_schema'], a)
        props = CAPS[capability_id]['input_schema'].get('properties', {})
        a = {k: (int(v) if props.get(k, {}).get('type') == 'integer' and v is not None else v) for k, v in a.items()}
        out = getattr(self, '_cmd_' + capability_id.replace('.', '_'))(**a)
        if CAPS[capability_id]['kind'] not in ('query', 'analysis', 'simulation'):
            self.revision += 1
        return out

    def _need_plant(self):
        if self.plant is None:
            raise ActionRejected('PLANT_REQUIRED')

    def _need_idle(self):
        self._need_plant()
        if self.op is not None:
            raise ActionRejected('OPERATION_RUNNING')

    def _need_power(self):
        if not self.power:
            raise ActionRejected('POWER_OFF')

    def _cmd_plant_configure(self, room_k, mains_voltage_v, noise_seed, vacuum_pump, cell_wash_installed, waste_capacity_m3):
        if self.plant is not None and noise_seed != self.plant['noise_seed']:
            raise ActionRejected('SEED_FIXED')
        if self.op is not None and (vacuum_pump != self.plant['vacuum_pump'] or cell_wash_installed != self.plant['cell_wash_installed']):
            raise ActionRejected('OPERATION_RUNNING')
        self.plant = dict(room_k=room_k, mains_voltage_v=mains_voltage_v, noise_seed=noise_seed, vacuum_pump=vacuum_pump, cell_wash_installed=cell_wash_installed,
                          waste_capacity_m3=waste_capacity_m3)
        if self._tube_bias is None:
            self._tube_bias = self._rng('tube_bias').normal(0.0, CV_TUBE, 96)
        if self.power and not (100.0 * 0.9 <= mains_voltage_v <= 240.0 * 1.1):
            self._power_off('MAINS_FAULT', '供电电压超出范围，电源断开')
        return dict(plant=dict(self.plant))

    def _power_off(self, code, msg):
        self.power = False
        if self.op is not None:
            self._fail_op('POWER_LOST')
        self._event('power', code, msg)

    def _set_power(self, enabled):
        self._need_plant()
        if enabled and not self.power:
            if not (100.0 * 0.9 <= self.plant['mains_voltage_v'] <= 240.0 * 1.1):
                raise ActionRejected('MAINS_OUT_OF_RANGE')
            self.power = True
            self._event('power', 'POWER_ON', '通电（自检后载板回初始位、洗头停放）')
            self.led['carrier_travel_m'].add(abs(self.carrier))
            self.led['manifold_travel_m'].add(abs(self.manifold))
            self.carrier, self.manifold = 0.0, 0.0
        elif not enabled and self.power:
            self._power_off('POWER_OFF', '断电')
        return dict(enabled=self.power)

    def _cmd_power_set(self, enabled):
        return self._set_power(enabled)

    def _cmd_power_press_switch(self):
        return self._set_power(not self.power)

    def _cmd_buffer_load(self, bottle, liquid_id, volume_m3):
        self._need_plant()
        if liquid_id not in liquid_lib()['substances']:
            raise ActionRejected('UNKNOWN_LIQUID')
        if self.op is not None and self.op.get('buffer') == bottle:
            raise ActionRejected('OPERATION_RUNNING')
        self.bottles[bottle] = dict(liquid=liquid_id, volume=float(volume_m3))
        self._event('fluid', 'BOTTLE_FILLED', f'供液瓶{bottle}：{liquid_id} {volume_m3 * 1e3:.2f} L')
        return dict(bottle=bottle, liquid_id=liquid_id, volume_m3=float(volume_m3))

    def _cmd_waste_empty(self):
        self._need_plant()
        if self.op is not None:
            raise ActionRejected('OPERATION_RUNNING')
        v = self.waste
        self.waste = 0.0
        self._event('fluid', 'WASTE_EMPTIED', f'倒空废液约{round(v / 50e-6) * 0.05:.2f} L')
        return dict(emptied_m3=round(v / 50e-6) * 50e-6)                   # read on the bottle's 50 mL graduations (the true volume is ledger truth)

    def _cmd_plate_provide(self, plate_id, plate_type, fill_volume_m3, liquid_id, unbound_mol, nonspecific_mol, specific_mol, cells_per_well, cell_adhesion, wells):
        self._need_plant()
        if plate_id in self.plates:
            raise ActionRejected('PLATE_EXISTS')
        if len(self.plates) >= MAX_PLATES:
            raise ActionRejected('TOO_MANY_PLATES')
        pt = plate_lib()['plates'].get(plate_type)
        if pt is None:
            raise ActionRejected('UNKNOWN_PLATE_TYPE')
        if not pt['compatible_with_96_tube_single_manifold']:
            raise ActionRejected('PLATE_INCOMPATIBLE')
        if liquid_id not in liquid_lib()['substances']:
            raise ActionRejected('UNKNOWN_LIQUID')
        if fill_volume_m3 > pt['well_volume_m3']:
            raise ActionRejected('WELL_OVERFILLED')
        if cells_per_well > 0 and cell_adhesion == 'none':
            raise ActionRejected('INVALID_ARGUMENT')
        used = np.zeros(96, bool)
        used[list(range(96)) if wells is None else wells] = True
        rng = self._rng(f'plate:{plate_id}')
        f = used.astype(float)
        self.plates[plate_id] = dict(
            location='bench', type=plate_type, liquid=liquid_id, used=used.tolist(), V=f * fill_volume_m3, unbound=f * unbound_mol, ns=f * nonspecific_mol,
            spec=f * specific_mol, cells=f * cells_per_well, cells0=f * cells_per_well, adhesion=cell_adhesion, contamination=np.zeros(96), comp=[{liquid_id: 1.0} for _ in range(96)],
            wbe=pt['well_bottom_elevation_m'] + rng.normal(0.0, WBE_SCATTER_M, 96), dry_s=np.zeros(96), ufrac=np.zeros(96), layer_V=np.zeros(96))
        self._event('plate', 'PLATE_PROVIDED', f'板{plate_id}（{plate_type}）放在实验台上')
        return dict(plate_id=plate_id, location='bench')

    def _cmd_plate_load(self, plate_id):
        self._need_idle()
        p = self.plates.get(plate_id)
        if p is None:
            raise ActionRejected('UNKNOWN_PLATE')
        if p['location'] != 'bench':
            raise ActionRejected('PLATE_NOT_ON_BENCH')
        if self.on_carrier is not None:
            raise ActionRejected('CARRIER_OCCUPIED')
        if abs(self.carrier) > 1e-12 or abs(self.manifold) > 1e-12:
            raise ActionRejected('CARRIER_NOT_HOME')
        p['location'] = 'carrier'
        self.on_carrier = plate_id
        self._event('plate', 'PLATE_LOADED', f'板{plate_id}放上载板', rid=None)
        return dict(plate_id=plate_id)

    def _cmd_plate_unload(self, plate_id):
        self._need_idle()
        if self.on_carrier != plate_id:
            raise ActionRejected('PLATE_NOT_ON_CARRIER')
        if abs(self.carrier) > 1e-12 or abs(self.manifold) > 1e-12:
            raise ActionRejected('CARRIER_NOT_HOME')
        self.plates[plate_id]['location'] = 'bench'
        self.on_carrier = None
        self._event('plate', 'PLATE_UNLOADED', f'板{plate_id}取下')
        return dict(plate_id=plate_id)

    # ---------------------------------------------------------------- operations: planning
    def _new_record(self, kind, prefix, key, settings, liquids):
        if len(self.records) >= MAX_RECORDS:
            raise ActionRejected('RECORDS_FULL')
        self.seq[key] += 1
        rid = f'{prefix}{self.seq[key]:04d}'
        self.records[rid] = dict(record_id=rid, kind=kind, status='running', started_s=self.t, finished_s=None, plate_id=self.on_carrier if kind == 'wash' else None,
                                 settings=copy.deepcopy(settings), cycles_done=0, programmed_dispense_m3=0.0, _dispensed_m3=0.0, _aspirated_m3=0.0, alarms=[], warnings=[],
                                 template_source=dict(liquids=sorted(liquids), plate=self.plates[self.on_carrier]['type'] if kind == 'wash' and self.on_carrier else None))
        return rid

    def _start(self, kind, phases, rid, **extra):
        self.seq['op'] += 1
        oid = f'op_{self.seq["op"]:04d}'
        t = self.t
        for ph in phases:
            ph['t_start'] = t
            t += ph['duration']
        self.op = dict(op=oid, kind=kind, t0=self.t, t_end=t, phases=phases, i=0, record_id=rid, **extra)
        self.operations[oid] = dict(phase='running', kind=kind, record_id=rid, op=oid)
        self._begin_phase()
        return dict(operation_id=oid)

    def _check_supply(self, bottle):
        if self._waste_full():
            raise ActionRejected('WASTE_FULL')
        if not self._fluid_ok(bottle):
            raise ActionRejected('BUFFER_EMPTY')

    def _cmd_prime_run(self, buffer, volume_m3, rate):
        self._need_idle()
        self._need_power()
        if abs(self.manifold) > 1e-12 or abs(self.carrier) > 1e-12:
            raise ActionRejected('CARRIER_NOT_HOME')
        if self.on_carrier is not None:
            raise ActionRejected('PLATE_ON_CARRIER')         # priming dispenses into the trough exposed with an empty carrier at home
        self._check_supply(buffer)
        if not self._vacuum_ok():
            raise ActionRejected('VACUUM_LOW')
        dur = volume_m3 / (96 * FLOW_RATE_M3_S[rate])
        rid = self._new_record('prime', 'PR', 'pr', dict(buffer=buffer, volume_m3=volume_m3, rate=rate), [self.bottles[buffer]['liquid']])
        phases = [dict(name='valve', duration=VALVE_S, z=0.0, x=0.0), dict(name='prime', duration=dur, z=-PARK_TIP_M + 0.005, x=0.0, volume=volume_m3, buffer=buffer),
                  dict(name='park', duration=0.5, z=0.0, x=0.0)]
        return self._start('prime', phases, rid, buffer=buffer)

    def _cmd_wash_run(self, protocol):
        self._need_idle()
        self._need_power()
        p = dict(protocol)
        if self.on_carrier is None:
            raise ActionRejected('NO_PLATE')
        if abs(self.carrier) > 1e-12 or abs(self.manifold) > 1e-12:
            raise ActionRejected('CARRIER_NOT_HOME')
        cw_disp, cw_trav = p['dispense_rate'] <= 2, p['travel_rate'].endswith('CW')
        if (cw_disp or cw_trav) and not self.plant['cell_wash_installed']:
            raise ActionRejected('CELL_WASH_OPTION_ABSENT')
        if abs(p['dispense_volume_m3'] / VOLUME_STEP_M3 - round(p['dispense_volume_m3'] / VOLUME_STEP_M3)) > 1e-6:
            raise ActionRejected('VOLUME_NOT_IN_1UL_STEPS')
        if p['vacuum_delay_m3'] > p['dispense_volume_m3']:
            raise ActionRejected('INVALID_ARGUMENT')
        self._check_supply(p['buffer'])
        if not self._vacuum_ok():
            raise ActionRejected('VACUUM_LOW')
        pt = plate_lib()['plates'][self.plates[self.on_carrier]['type']]
        speed = TRAVEL[p['travel_rate']][0]
        z_disp, z_asp = p['dispense_z_steps'] * Z_STEP_M, p['aspirate_z_steps'] * Z_STEP_M
        z_sec = None if p['secondary_aspirate_z_steps'] is None else p['secondary_aspirate_z_steps'] * Z_STEP_M
        Q = FLOW_RATE_M3_S[p['dispense_rate']]
        t_disp = p['dispense_volume_m3'] / Q
        down = max(0.0, z_disp - z_asp) / speed
        aspirate = [dict(name='aspirate', duration=down + p['aspirate_delay_s'] + 0.3, z=z_asp - PARK_TIP_M,
                         speed=speed, height=z_asp, secondary=z_sec)]
        if z_sec is not None:
            aspirate.append(dict(name='aspirate_secondary', duration=abs(z_asp - z_sec) / speed + 0.6, z=z_sec - PARK_TIP_M, speed=speed, height=z_sec))
        dispense = dict(name='dispense', duration=VALVE_S + (z_disp - min(z_asp, z_sec or z_asp)) / LIFT_SPEED_M_S + t_disp, z=z_disp - PARK_TIP_M, rate=p['dispense_rate'],
                        volume=p['dispense_volume_m3'], height=z_disp, vac_delay=p['vacuum_delay_m3'], buffer=p['buffer'])
        phases = [dict(name='move_to_manifold', duration=CARRIER_MOVE_S, z=0.0, x=-CARRIER_TRAVEL_M)]
        if p['bottom_wash']:
            phases.append(dict(name='bottom_wash', duration=VALVE_S + (z_disp - z_asp) / LIFT_SPEED_M_S + 2 * p['dispense_volume_m3'] / Q + 0.5, z=z_asp - PARK_TIP_M,
                               rate=max(3, p['dispense_rate']), volume=p['dispense_volume_m3'], height=z_asp, buffer=p['buffer'], speed=speed))
        for c in range(p['cycles']):
            phases += [dict(ph, cycle=c) for ph in aspirate] + [dict(dispense, cycle=c)]
            home = (p['soak_s'] + p['shake_s']) > HOME_AFTER_S
            if p['shake_s'] > 0:
                phases.append(dict(name='shake', duration=p['shake_s'] + (2 * CARRIER_MOVE_S if home else 0.0), z=0.0, x=0.0 if home else -CARRIER_TRAVEL_M,
                                   intensity=p['shake_intensity'], cycle=c, home=home))
            if p['soak_s'] > 0:
                phases.append(dict(name='soak', duration=p['soak_s'], z=0.0, x=0.0 if home else -CARRIER_TRAVEL_M, cycle=c, home=home))
            if home and (p['soak_s'] > 0 or p['shake_s'] > 0):
                phases.append(dict(name='move_to_manifold', duration=CARRIER_MOVE_S, z=0.0, x=-CARRIER_TRAVEL_M))
        if p['final_aspirate']:
            phases += [dict(ph, cycle=p['cycles'], final=True) for ph in aspirate]
        phases.append(dict(name='move_home', duration=CARRIER_MOVE_S, z=0.0, x=0.0))
        liquids = [self.bottles[p['buffer']]['liquid']] + list(self.line)
        rid = self._new_record('wash', 'WS', 'ws', p, liquids)
        if pt['well_volume_m3'] < p['dispense_volume_m3']:
            self.records[rid]['warnings'].append('dispense_volume_exceeds_well_volume')
        return self._start('wash', phases, rid, buffer=p['buffer'], protocol=p)

    def _cmd_maintenance_clean_tubes(self):
        self._need_idle()
        rid = self._new_record('clean', 'CL', 'cl', {}, [])
        phases = [dict(name='clean', duration=600.0, z=0.0, x=0.0)]
        return self._start('clean', phases, rid)

    # ---------------------------------------------------------------- operations: execution
    def _begin_phase(self):
        o = self.op
        ph = o['phases'][o['i']]
        self.phase = ph['name']
        d = abs(ph.get('x', self.carrier) - self.carrier)
        self.led['carrier_travel_m'].add(d)
        self.led['manifold_travel_m'].add(abs(ph.get('z', self.manifold) - self.manifold))
        self.carrier = ph.get('x', self.carrier)
        self.manifold = ph.get('z', self.manifold)
        if ph['name'] == 'valve' or ph['name'] in ('dispense', 'bottom_wash', 'prime'):
            self.valve = ph.get('buffer', self.valve)

    def _next_event_t(self):
        o = self.op
        if o is None:
            return None
        ph = o['phases'][o['i']]
        return ph['t_start'] + ph['duration']

    def step(self, dt_s):
        if not (isinstance(dt_s, (int, float)) and math.isfinite(dt_s) and dt_s >= 0):
            raise ActionRejected('INVALID_ARGUMENT')
        t_end = self.t + dt_s
        while True:
            nxt = self._next_event_t()
            if nxt is None or nxt > t_end + 1e-12:
                self._advance(t_end - self.t)
                break
            self._advance(max(0.0, nxt - self.t))
            self._finish_phase()
        return dict(time_s=self.t)

    def _advance(self, h):
        if h <= 0:
            return
        if self.power:
            self.led['electrical_energy_j'].add((ACTIVE_W if self.op is not None and self.op['kind'] != 'clean' else IDLE_W) * h)
        if self.op is None or self.op['kind'] == 'clean':
            self._idle(h)
        self._drip(h)
        self._dry(h)
        self.t += h

    def _reset_idle(self):
        """Fresh liquid in the tubes: restart the idle clock and draw each tube's clogging threshold (unit exponential) and residual flow; each tube
        tip holds DROPS_PER_TIP drops that can fall later (siphoned from the selected bottle through the line; booked in dripped_m3)."""
        self.idle_s, self.idle_H, self.idle_n = 0.0, 0.0, self.idle_n + 1
        self.clog_E = None
        self.drip_left[:] = DROPS_PER_TIP

    def _idle(self, h):
        """Idle manifold: liquid dries in the tube tips; each dispense and aspirate tube clogs with the hazard rate 0.5 / tau, tau = CLOG_TAU_S (1 %
        salt) / salt fraction of the liquid left in the tubes.  The thresholds are drawn once per idle period, so the result does not depend on how the
        idle time is split into steps."""
        self.idle_s += h
        s = salt_fraction(self.tip_liquid)
        if s <= 0 or self.plant is None:
            return
        if self.clog_E is None:
            rng = self._rng(f'clog:{self.idle_n}')
            self.clog_E = (rng.exponential(1.0, 96), rng.exponential(1.0, 96), rng.uniform(0.0, 0.7, 96))
        H0 = self.idle_H
        self.idle_H += 0.5 * h / (CLOG_TAU_S * 0.01 / s)
        Ed, Ea, frac = self.clog_E
        for i in np.where((Ed > H0) & (Ed <= self.idle_H))[0]:
            self.clog_d[int(i)] = min(self.clog_d.get(int(i), 1.0), float(frac[i]))
        for i in np.where((Ea > H0) & (Ea <= self.idle_H))[0]:
            self.clog_a.add(int(i))

    def _drip(self, h):
        """A plate left under the parked manifold catches drops from the tube tips (the guide sends the carrier home for soaks > 1 min): a Poisson
        process per tube in the exposure time, with the drop times drawn per tube and drop so that the result does not depend on the step size."""
        p = self.plates.get(self.on_carrier)
        if p is None or abs(self.carrier + CARRIER_TRAVEL_M) > 1e-9 or self.phase not in ('soak', 'shake', None):
            return
        seed = self.plant['noise_seed'] if self.plant else 0
        if self.drip_next is None:
            self.drip_next = [(float(np.random.default_rng([seed, 7, i, 0]).exponential(1 / DRIP_P_PER_S)), 0) for i in range(96)]
        end = self.drip_clock + h
        n = 0
        pt = plate_lib()['plates'][p['type']]
        cap = pt['well_volume_m3'] + math.pi * pt['mean_well_radius_m'] ** 2 * MENISCUS_M * 0.5
        spilled, lost = np.zeros(96), np.zeros(96)
        for i in range(96):
            t, k = self.drip_next[i]
            while t <= end and self.drip_left[i] > 0 and self.bottles[self.valve]['volume'] >= DROP_M3:
                self.drip_left[i] -= 1                                   # the drop siphons out of the line: the selected bottle loses it
                self.bottles[self.valve]['volume'] -= DROP_M3
                self.led['dripped_m3'].add(DROP_M3)                        # clean line buffer: it dilutes, it carries no analyte
                if p['V'][i] + DROP_M3 > cap:                                # a full well runs over (the drop's volume leaves at the rim)
                    spilled[i] += p['V'][i] + DROP_M3 - cap
                    keep = cap / (p['V'][i] + DROP_M3)
                    lost[i] += (p['unbound'][i] + p['contamination'][i]) * (1 - keep)
                    p['unbound'][i] *= keep
                    p['contamination'][i] *= keep
                    p['V'][i] = cap
                else:
                    p['V'][i] += DROP_M3
                n += 1
                k += 1
                t += float(np.random.default_rng([seed, 7, i, k]).exponential(1 / DRIP_P_PER_S))
            while t <= end:                                              # the tip is dry: the drop times still advance
                k += 1
                t += float(np.random.default_rng([seed, 7, i, k]).exponential(1 / DRIP_P_PER_S))
            self.drip_next[i] = (t, k)
        self.drip_clock = end
        if spilled.any():
            self._spill(p, spilled, self.records.get(self.op['record_id']) if self.op else None, lost)
        if n:
            self._event('plate', 'DRIP', f'{n}滴从洗头管尖落入板孔')

    def _tip_conc(self):
        return self.op['tip_conc'] if self.op and 'tip_conc' in self.op else 0.0

    def _dry(self, h):
        """Wells that are almost empty dry out; adherent cells in them are damaged after DRY_DWELL_S."""
        for p in self.plates.values():
            low = (p['V'] < 3e-9) & (p['cells'] > 0)
            p['dry_s'][low] += h
            p['dry_s'][~low] = 0.0
            bad = p['dry_s'] > DRY_DWELL_S
            if bad.any():
                p['cells'][bad] *= math.exp(-h / 60.0)

    def _finish_phase(self):
        o = self.op
        ph = o['phases'][o['i']]
        rec = self.records[o['record_id']]
        ok = self._apply_phase(ph, rec)
        if not ok:
            return
        o['i'] += 1
        if o['i'] >= len(o['phases']):
            self._complete()
        else:
            self._begin_phase()

    def _apply_phase(self, ph, rec):
        n = ph['name']
        if n == 'prime':
            return self._do_prime(ph, rec)
        if n in ('aspirate', 'aspirate_secondary'):
            return self._do_aspirate(ph, rec)
        if n == 'dispense':
            return self._do_dispense(ph, rec)
        if n == 'bottom_wash':
            return self._do_bottom_wash(ph, rec) and self._do_aspirate(ph, rec) and self._do_dispense(dict(ph, vac_delay=ph['volume']), rec, count=False)
        if n == 'shake':
            self._mix(ph['duration'], SHAKE_TAU[ph['intensity']], shake=True)
            return True
        if n == 'soak':
            self._mix(ph['duration'], None, shake=False)
            return True
        if n == 'clean':
            n_cl = len(self.clog_d) + len(self.clog_a)
            self.clog_d.clear()
            self.clog_a.clear()
            self._event('maintenance', 'TUBES_CLEANED', f'清洁针疏通{n_cl}处堵塞')
            return True
        return True

    @property
    def line(self):
        """Composition of the liquid at the manifold end of the fluid path (the last tank)."""
        return self.tanks[-1]

    def _draw(self, bottle, V, rec):
        """Take up to V from a supply bottle through the fluid path (LINE_TANKS stirred tanks in series, total dead volume 110 mL).  Returns the mean
        composition of the liquid that leaves at the manifold and the volume drawn; a bottle that runs dry mid-draw delivers what it holds and raises
        BUFFER_EMPTY (fluid sensor); (None, 0) when it is empty from the start."""
        b = self.bottles[bottle]
        if not self._fluid_ok(bottle):
            self._alarm(rec, 'BUFFER_EMPTY')
            return None, 0.0
        V_take = min(V, b['volume'])
        Vt = DEAD_VOLUME_M3 / LINE_TANKS
        n = max(1, int(math.ceil(V_take / (Vt / 40))))
        dv = V_take / n
        out = {}
        for _ in range(n):
            inflow = {b['liquid']: 1.0}
            for j, tank in enumerate(self.tanks):
                new = {k: x * (1 - dv / Vt) for k, x in tank.items()}
                for k, x in inflow.items():
                    new[k] = new.get(k, 0.0) + x * dv / Vt
                inflow = tank                                            # the outflow of this tank (its composition before the step) feeds the next
                self.tanks[j] = new
            for k, x in inflow.items():
                out[k] = out.get(k, 0.0) + x * dv / max(V_take, 1e-30)
        self.tanks = [{k: x / sum(t.values()) for k, x in t.items() if x > 1e-12} for t in self.tanks]
        b['volume'] -= V_take
        if V_take < V * (1 - 1e-12):
            self._alarm(rec, 'BUFFER_EMPTY')
        tot = sum(out.values()) or 1.0
        return {k: x / tot for k, x in out.items() if x / tot > 1e-12}, V_take

    def _alarm(self, rec, code):
        if code not in rec['alarms']:
            rec['alarms'].append(code)
        self._event('alarm', code, {'WASTE_FULL': '废液瓶满，运行停止', 'VACUUM_LOW': '真空不足，抽吸失败', 'BUFFER_EMPTY': '所选供液瓶无液',
                                    'FLOW_ERROR': '分配总流量低于设定（可能堵针）', 'POWER_LOST': '断电'}[code], rid=rec['record_id'])
        if code in ('WASTE_FULL', 'VACUUM_LOW', 'BUFFER_EMPTY', 'POWER_LOST'):
            self._fail_op(code)

    def _do_prime(self, ph, rec):
        if self._waste_full() or self.waste + ph['volume'] > self.plant['waste_capacity_m3']:   # the prime volume must fit into the bottles (C4)
            self._alarm(rec, 'WASTE_FULL')
            return False
        comp, got = self._draw(ph['buffer'], ph['volume'], rec)
        if comp is None:
            return False
        rec['_dispensed_m3'] += got
        rec['programmed_dispense_m3'] += ph['volume']
        self.led['primed_m3'].add(got)
        self._to_waste(got)
        self.tip_liquid = dict(self.line)
        self._reset_idle()
        return got >= ph['volume'] * (1 - 1e-12)

    def _to_waste(self, V):
        self.waste += V
        self.led['waste_added_m3'].add(V)

    def _aspirated(self, rec, V):
        rec['_aspirated_m3'] += V
        self.led['aspirated_m3'].add(V)
        self._to_waste(V)

    def _do_aspirate(self, ph, rec):
        if not self._vacuum_ok():
            self._alarm(rec, 'VACUUM_LOW')
            return False
        p = self.plates[self.on_carrier]
        if self._waste_full() or self.waste + float(np.sum(p['V'])) > self.plant['waste_capacity_m3']:   # the plate's liquid must fit (C4)
            self._alarm(rec, 'WASTE_FULL')
            return False
        pt = plate_lib()['plates'][p['type']]
        A = math.pi * pt['mean_well_radius_m'] ** 2
        speed = ph['speed']
        delay = self.op['protocol'].get('aspirate_delay_s', 0.0) if self.op else 0.0
        dist = TRAVEL[self.op['protocol']['travel_rate']][1]
        rng = self._rng(f'asp:{rec["record_id"]}:{self.op["i"]}')
        removed = 0.0
        tip_c = []
        for i in range(96):
            V = p['V'][i]
            if V <= 0:
                continue
            gap = ph['height'] - p['wbe'][i]
            if gap < -0.1e-3:
                if 'tubes_touch_well_bottom' not in rec['warnings']:
                    rec['warnings'].append('tubes_touch_well_bottom')
            if i in self.clog_a:
                continue
            rho, mu, sig = mixture_props(p['comp'][i], self._T())
            res = residual_volume(V, gap, pt, rho, mu, sig, speed, delay) * float(np.exp(rng.normal(0.0, RESIDUAL_SCATTER)))
            res = min(V, res)
            u, Lv = p['ufrac'][i], min(p['layer_V'][i], V)
            # the unmixed layer (volume Lv, the fraction u of the species) lies at the bottom and is left first; the mixed fraction 1 - u is
            # spread uniformly over the whole volume V, so the residual res keeps (1 - u) res / V of it
            if res <= Lv:
                f = u * res / max(Lv, 1e-18) + (1 - u) * res / V
            else:
                f = u + (1 - u) * res / V
            conc = (p['unbound'][i] + p['contamination'][i]) / V
            tip_c.append(conc)
            p['unbound'][i] *= f
            p['contamination'][i] *= f
            p['ufrac'][i], p['layer_V'][i] = 0.0, 0.0
            removed += V - res
            p['V'][i] = res
            if p['cells'][i] > 0:                                           # aspiration shear / scraping near the cell layer
                scrape = max(0.0, 1 - max(gap, 0.0) / CELL_LAYER_CLEAR_M) * 0.25 * dist
                suction = 0.02 * dist * (speed / 4e-3)
                strong = 0.25 if p['adhesion'] == 'strong' else 1.0
                p['cells'][i] *= math.exp(-(scrape + suction) * strong)
        self._aspirated(rec, removed)
        self.op['tip_conc'] = float(np.mean(tip_c)) if tip_c else 0.0
        if ph.get('final') and ph['name'] == 'aspirate':
            rec['cycles_done'] = max(rec['cycles_done'], self.op['protocol']['cycles'])
        return True

    def _do_dispense(self, ph, rec, count=True):
        p = self.plates[self.on_carrier]
        pt = plate_lib()['plates'][p['type']]
        rate = ph['rate']
        rng = self._rng(f'disp:{rec["record_id"]}:{self.op["i"]}')
        cv = math.sqrt(CV_REPEAT ** 2 + (CV_ABS_M3 / ph['volume']) ** 2)
        want = np.array([max(0.0, ph['volume'] * self.clog_d.get(i, 1.0) * (1 + self._tube_bias[i] + rng.normal(0.0, cv))) for i in range(96)])
        comp, got = self._draw(ph['buffer'], float(want.sum()), rec)       # the bottle gives what the tubes actually deliver (C1)
        if comp is None:
            return False
        vols = want * (got / want.sum()) if want.sum() > 0 else want       # a bottle running dry shares its rest over the tubes
        rho, mu, sig = mixture_props(comp, self._T())
        m_jet = 1 - (1 - MIX_MIN) * math.exp(-jet_speed(rate) / MIX_JET_M_S)
        A = math.pi * pt['mean_well_radius_m'] ** 2
        top = pt['height_m']
        tip = ph['height']
        vac = self._vacuum_ok() and ph['vac_delay'] < ph['volume']
        bulge = A * MENISCUS_M * 0.5
        spilled, lost = np.zeros(96), np.zeros(96)
        skimmed = 0.0
        for i in range(96):
            v = float(vols[i])
            V0 = p['V'][i]
            V1 = V0 + v
            # the residual mixes into the fresh buffer with the jet efficiency; the unmixed rest stays as a layer at the bottom
            if v > 0:   # the jet entrains the old liquid V0 into the fresh v; the part left unmixed (1 - m_jet) V0 / (V0 + v) stays as a bottom layer
                p['ufrac'][i] = (1 - m_jet) * V0 / (V0 + v)
                p['layer_V'][i] = V0
            # with the vacuum on, this well's aspirate tip skims what rises above it (tip below the rim: the level stops at the tip; tip up to
            # 1.5 mm above the rim: at the pinned meniscus); a blocked aspirate tube does not skim and its well runs over the rim (S1, S2)
            skim_here = vac and i not in self.clog_a and tip <= top + MENISCUS_M
            if not skim_here:
                v_cap = pt['well_volume_m3'] + bulge
            elif tip < top:
                v_cap = min(pt['well_volume_m3'], A * max(tip - p['wbe'][i], 0.0))
            else:
                v_cap = pt['well_volume_m3'] + bulge * (tip - top) / MENISCUS_M
            if V1 > v_cap:
                excess = V1 - v_cap
                if skim_here:
                    skimmed += excess
                else:
                    spilled[i] = excess
                u = p['ufrac'][i]                                           # the liquid leaving at the top carries the mixed fraction only,
                tot = u + (1 - u) * math.exp(-excess / v_cap)               # washed out continuously while the jet runs (stirred-tank overflow)
                if not skim_here:
                    lost[i] = (p['unbound'][i] + p['contamination'][i]) * (1 - tot)
                p['unbound'][i] *= tot
                p['contamination'][i] *= tot
                p['ufrac'][i] = u / tot if tot > 0 else 0.0
                p['layer_V'][i] = min(p['layer_V'][i], v_cap)
                V1 = v_cap
            p['V'][i] = V1
            p['comp'][i] = self._mix_comp(p['comp'][i], V0, comp, v)
            if p['cells'][i] > 0:
                tau = wall_shear(rate, V0 / A, rho)
                strong = 4.0 if p['adhesion'] == 'strong' else 1.0
                p['cells'][i] *= math.exp(-(tau / (TAU_CELL_PA * strong)) ** 2)
        if spilled.any():
            self._spill(p, spilled, rec, lost)
        if skimmed > 0:
            self._aspirated(rec, skimmed)
        self.led['dispensed_m3'].add(got)
        rec['_dispensed_m3'] += got
        rec['programmed_dispense_m3'] += 96 * ph['volume']
        full = got >= float(want.sum()) * (1 - 1e-12)
        self.flow_ok = bool(got >= 0.9 * 96 * ph['volume'])
        if not self.flow_ok and full:
            self._alarm(rec, 'FLOW_ERROR')
        self.tip_liquid = comp
        self._reset_idle()
        self._desorb(p, contact=ph.get('duration', 3.0), surf=is_surfactant(comp))
        if count and full:
            rec['cycles_done'] = max(rec['cycles_done'], ph.get('cycle', 0) + 1)
            self.led['cycles'].add(1)
        return full

    def _do_bottom_wash(self, ph, rec):
        """Bottom wash (guide p.45): fluid dispensed and aspirated at the same time at the aspirate height - a flow-through of the volume v through the
        liquid held below the tips, n <- n exp(-m v / V_hold), with the jet close to the bottom (vigorous: cell layers suffer)."""
        if not self._vacuum_ok():
            self._alarm(rec, 'VACUUM_LOW')
            return False
        p = self.plates[self.on_carrier]
        pt = plate_lib()['plates'][p['type']]
        A = math.pi * pt['mean_well_radius_m'] ** 2
        want = np.array([ph['volume'] * self.clog_d.get(i, 1.0) for i in range(96)])
        comp, got = self._draw(ph['buffer'], float(want.sum()), rec)
        if comp is None:
            return False
        vols = want * (got / want.sum()) if want.sum() > 0 else want
        rho, mu, sig = mixture_props(comp, self._T())
        m_jet = 1 - (1 - MIX_MIN) * math.exp(-jet_speed(ph['rate']) / MIX_JET_M_S)
        cap = pt['well_volume_m3'] + A * MENISCUS_M * 0.5
        removed = 0.0
        spilled, lost = np.zeros(96), np.zeros(96)
        for i in range(96):
            v = float(vols[i])
            hold = max(A * max(ph['height'] - p['wbe'][i], 0.0) + A * DELTA0_M, 1e-12)
            if i in self.clog_a:                                         # no aspiration: the well fills and runs over the rim if needed
                V0 = p['V'][i]
                p['comp'][i] = self._mix_comp(p['comp'][i], V0, comp, v)
                if V0 + v > cap:
                    spilled[i] = V0 + v - cap
                    keep = cap / (V0 + v)
                    lost[i] = (p['unbound'][i] + p['contamination'][i]) * (1 - keep)
                    p['unbound'][i] *= keep
                    p['contamination'][i] *= keep
                p['V'][i] = min(V0 + v, cap)
                continue
            f = math.exp(-m_jet * v / hold)                               # flow-through; what leaves through the tip goes to waste (C2)
            p['unbound'][i] *= f
            p['contamination'][i] *= f
            V_new = min(p['V'][i], hold)
            removed += p['V'][i] + v - V_new
            p['V'][i] = V_new
            p['comp'][i] = dict(comp)
            if p['cells'][i] > 0:
                strong = 4.0 if p['adhesion'] == 'strong' else 1.0
                p['cells'][i] *= math.exp(-(wall_shear(ph['rate'], hold / A, rho) / (TAU_CELL_PA * strong)) ** 2)
        if spilled.any():
            self._spill(p, spilled, rec, lost)
        rec['_dispensed_m3'] += got
        rec['programmed_dispense_m3'] += 96 * ph['volume']
        self.led['dispensed_m3'].add(got)
        self._aspirated(rec, removed)
        self.tip_liquid = comp
        self._reset_idle()
        return got >= float(want.sum()) * (1 - 1e-12)

    @staticmethod
    def _mix_comp(c0, V0, c1, v):
        tot = V0 + v
        if tot <= 0:
            return dict(c1)
        out = {k: x * V0 / tot for k, x in c0.items()}
        for k, x in c1.items():
            out[k] = out.get(k, 0.0) + x * v / tot
        return {k: x for k, x in out.items() if x > 1e-9}

    def _spill(self, p, spilled, rec, lost):
        """Liquid above the rim runs over into the four neighbouring wells (and the carrier): 10 % of the soluble species that left each well over
        the rim (lost, mol) reach its neighbours (the same rule for the dispense, bottom-wash and drip overflows)."""
        if rec is not None and 'overflow_observed' not in rec['warnings']:   # no overflow sensor: a visible observation, not an instrument alarm
            rec['warnings'].append('overflow_observed')
        self._event('plate', 'OVERFLOW', '孔内液体溢出（可见，仪器无传感器）', rid=rec['record_id'] if rec is not None else None)
        total = float(spilled.sum())
        self.led['overflow_m3'].add(total)
        for i in np.where(spilled > 0)[0]:
            c, r = divmod(int(i), 8)
            nb = [8 * cc + rr for cc, rr in ((c - 1, r), (c + 1, r), (c, r - 1), (c, r + 1)) if 0 <= cc < 12 and 0 <= rr < 8]
            for j in nb:
                p['contamination'][j] += 0.1 * lost[i] / len(nb)

    def _desorb(self, p, contact, surf):
        k_ns = K_OFF_NS_SURF if surf else K_OFF_NS
        d_ns = p['ns'] * (1 - math.exp(-k_ns * contact))
        d_sp = p['spec'] * (1 - math.exp(-K_OFF_SPEC * contact))
        p['ns'] -= d_ns
        p['spec'] -= d_sp
        before = p['unbound'] + p['contamination']
        p['unbound'] += d_ns + d_sp
        after = p['unbound'] + p['contamination']
        p['ufrac'] = np.where(after > 0, p['ufrac'] * before / np.maximum(after, 1e-300), 0.0)   # desorbed species enter the bulk

    def _mix(self, t, tau, shake):
        """Soak / shake: mixing of the residual layer with the bulk (shake), desorption of adsorbed species (both); the jet mixing deficit of the
        last dispense decays with the shake time constant."""
        p = self.plates.get(self.on_carrier)
        if p is None:
            return
        p['ufrac'] *= math.exp(-t / (tau if shake else SOAK_TAU_S))
        self._desorb(p, contact=t, surf=is_surfactant(self.tip_liquid))
        if shake and (p['cells'] > 0).any():
            strong = 4.0 if p['adhesion'] == 'strong' else 1.0
            p['cells'] *= math.exp(-t / (3600.0 * strong) * (3.0 / tau))

    def _complete(self):
        o = self.op
        rec = self.records[o['record_id']]
        rec['status'] = 'completed'
        rec['finished_s'] = self.t
        self.operations[o['op']]['phase'] = 'completed'
        self.phase = None
        self.carrier, self.manifold = 0.0, 0.0
        self._event('program', 'RUN_DONE', f'{rec["kind"]}记录{rec["record_id"]}完成', rid=rec['record_id'])
        self.op = None

    def _fail_op(self, code):
        o = self.op
        if o is None:
            return
        rec = self.records[o['record_id']]
        if code not in rec['alarms']:
            rec['alarms'].append(code)
        rec['status'] = 'failed'
        rec['finished_s'] = self.t
        self.operations[o['op']]['phase'] = 'failed'
        self.phase = None
        self.op = None
        if self.power:                                                     # the instrument homes both axes after an error (booked travel)
            self.led['carrier_travel_m'].add(abs(self.carrier))
            self.led['manifold_travel_m'].add(abs(self.manifold))
            self.carrier, self.manifold = 0.0, 0.0

    def _cancel_op(self, op):
        o = self.op
        if o is None or self.operations.get(o['op']) is not op:
            raise ActionRejected('NOT_RUNNING')
        rec = self.records[o['record_id']]
        rec['status'] = 'cancelled'
        rec['finished_s'] = self.t
        self.operations[o['op']]['phase'] = 'cancelled'
        self._event('program', 'CANCELLED', '运行取消（洗头停放、载板回初始位）', rid=rec['record_id'])
        self.led['carrier_travel_m'].add(abs(self.carrier))
        self.led['manifold_travel_m'].add(abs(self.manifold))
        self.carrier, self.manifold = 0.0, 0.0
        self.phase = None
        self.op = None

    # ---------------------------------------------------------------- queries, faults, time
    def _cmd_log_read(self):
        return dict(entries=[dict(record_id=r['record_id'], kind=r['kind'], status=r['status'], cycles_done=r['cycles_done'], alarms=list(r['alarms']),
                                  finished_s=r['finished_s']) for r in self.records.values()])

    @staticmethod
    def _out(r):
        """The published record: settings, cycles, programmed dispense volume, alarms, warnings - the washer does not measure the volumes it actually
        delivered or aspirated (those stay private and in the evaluator ledger)."""
        return {k: copy.deepcopy(v) for k, v in r.items() if not k.startswith('_')}

    def _cmd_measurement_result(self, record_id):
        r = self.records.get(record_id)
        if r is None:
            raise ActionRejected('UNKNOWN_RECORD')
        return self._out(r)

    def _cmd_records_list(self):
        return dict(records=sorted(self.records))

    def _cmd_events_read(self, since_s):
        return dict(events=[{k: v for k, v in e.items() if k != '_rid'} for e in self.events if e['time_s'] >= since_s], dropped=self.events_dropped)

    def ledger(self):
        return {k: v.value() for k, v in self.led.items()}

    def _cmd_ledger_read(self):
        return dict(ledger=self.ledger())

    def _cmd_plate_inspect(self, plate_id):
        p = self.plates.get(plate_id)
        if p is None:
            raise ActionRejected('UNKNOWN_PLATE')
        return dict(plate_id=plate_id, volume_m3=[float(v) for v in p['V']], unbound_mol=[float(v) for v in p['unbound']], nonspecific_mol=[float(v) for v in p['ns']],
                    specific_mol=[float(v) for v in p['spec']], cells=[float(v) for v in p['cells']], contamination_mol=[float(v) for v in p['contamination']])

    def _cmd_provenance_read(self):
        return dict(libraries=[dict(path=str(q.relative_to(ROOT)), sha256=hashlib.sha256(q.read_bytes()).hexdigest(), count=json.loads(q.read_text())['count'])
                               for q in (LIQUID_LIB_PATH, PLATE_LIB_PATH)])

    def _cmd_fault_inject(self, fault, tube, active):
        self._need_plant()
        if fault in ('dispense_tube_clog', 'aspirate_tube_clog'):
            if tube is None:
                raise ActionRejected('INVALID_ARGUMENT')
            if fault == 'dispense_tube_clog':
                (self.clog_d.__setitem__(tube, 0.0) if active else self.clog_d.pop(tube, None))
            else:
                (self.clog_a.add(tube) if active else self.clog_a.discard(tube))
        elif fault == 'power_loss':
            if active and self.power:
                self._power_off('POWER_LOST', '断电（故障注入）')
        else:
            (self.faults.add if active else self.faults.discard)(fault)
            if active and fault == 'vacuum_leak' and self.op is not None and self.phase in ('aspirate', 'aspirate_secondary', 'bottom_wash', 'prime'):
                self._alarm(self.records[self.op['record_id']], 'VACUUM_LOW')
        self._event('fault', 'FAULT_' + ('ON' if active else 'OFF'), f'{fault} {tube if tube is not None else ""}')
        return dict(faults=sorted(self.faults | {f'dispense_clog_{i}' for i in self.clog_d} | {f'aspirate_clog_{i}' for i in self.clog_a})[:8])

    def _cmd_sim_step(self, dt_s):
        self.step(dt_s)
        return dict(time_s=self.t)


# ---------------------------------------------------------------- sessions (instance, identity, idempotent requests) - as CHEM-162
ASYNC = {'prime.run', 'wash.run', 'maintenance.clean_tubes'}
_DATA_GUARDED = {'measurement.result'}
_RECORD_ARG = {'measurement.result': 'record_id'}
_CONTROL_GUARDED = {k for k, v in CAPS.items() if v['visibility'] == 'agent' and v['kind'] in ('device_command', 'robot_skill') and k not in UNSUPPORTED}


class Biotek405tsSession:
    def __init__(self, host, principal_id):
        self._host = host
        self._principal = principal_id

    @property
    def _role(self):
        return self._host._principals[self._principal]

    def _target(self, instance_id):
        if not isinstance(instance_id, str) or instance_id != self._host.instance_id:
            raise ActionRejected('UNKNOWN_INSTANCE')

    def describe(self, *, instance_id=None, asset_type_id=None):
        if (instance_id is None) == (asset_type_id is None):
            raise ActionRejected('INVALID_ARGUMENT')
        if instance_id is not None:
            self._target(instance_id)
        if asset_type_id is not None and asset_type_id != ASSET_TYPE_ID:
            raise ActionRejected('UNKNOWN_INSTANCE')
        meta = copy.deepcopy(export_biotek_405ts_contract_metadata())
        caps = {k: v for k, v in meta['capabilities'].items() if v['visibility'] == 'agent' or self._role != 'agent'}
        slots = {k: v for k, v in meta['state_slots'].items() if v['visibility'] == 'agent' or self._role != 'agent'}
        if self._role == 'agent':
            for c in caps.values():
                c['state_effects'] = [e for e in c['state_effects'] if e['state_id'] in slots]
                c['observes'] = [x for x in c['observes'] if x in slots]
                for pre in c['preconditions']:
                    pre['state_ids'] = [x for x in pre['state_ids'] if x in slots]
                c['execution']['completion_state_ids'] = [x for x in c['execution']['completion_state_ids'] if x in slots]
            for m in meta['mechanisms'].values():
                m['state_ids'] = [x for x in m['state_ids'] if x in slots]
        return dict(protocol_version=PROTOCOL_VERSION, asset_type_id=ASSET_TYPE_ID, asset_version='1.0.0', capabilities=caps, state_slots=slots)

    def _foreign(self, object_id):
        owner = self._host._owners.get(object_id)
        return owner is not None and owner != self._principal and self._host._principals.get(owner) == 'agent'

    def _foreign_op(self):
        h = self._host
        return h.op is not None and self._foreign(h.op['op'])

    def read(self, *, instance_id, state_ids=None):
        self._target(instance_id)
        h = self._host
        values = h.read(self._role)
        values['operation.started_by_other_agent'] = self._role == 'agent' and self._foreign_op()
        if self._role == 'agent':
            values['result.last_record_id'] = h._last_record(lambda k: not self._foreign(k))
        ids = list(values) if state_ids is None else state_ids
        if not isinstance(ids, list) or any(not isinstance(k, str) for k in ids):
            raise ActionRejected('INVALID_ARGUMENT')
        if any(k not in values for k in ids):
            raise ActionRejected('ACCESS_DENIED')
        return dict(instance_id=instance_id, status='succeeded', state_revision=h.revision, clock={'domain': 'simulation', 'time_s': h.time_s},
                    observations={k: dict(value=copy.deepcopy(values[k]), unit=SLOTS[k]['unit'],
                                          quality='unavailable' if values[k] is None else 'estimated' if SLOTS[k]['origin'] in ('sensor', 'derived') else 'valid',
                                          sample_time_s=h.time_s, sample_window_s=None) for k in ids})

    def _guard(self, capability_id, arguments):
        if self._role != 'agent':
            return
        h = self._host
        if capability_id in _CONTROL_GUARDED and self._foreign_op():
            if capability_id in ('power.set', 'power.press_switch') and not h.power:
                return
            raise ActionRejected('ACCESS_DENIED')
        if capability_id in _DATA_GUARDED and self._foreign(arguments.get(_RECORD_ARG[capability_id])):
            raise ActionRejected('ACCESS_DENIED')

    def invoke(self, *, instance_id, capability_id, arguments, request_id):
        h = self._host
        key = digest = None
        try:
            self._target(instance_id)
            if not isinstance(request_id, str) or not request_id:
                raise ActionRejected('INVALID_ARGUMENT')
            key = (self._principal, request_id)
            try:
                digest = json.dumps([capability_id, arguments], sort_keys=True, allow_nan=False)
            except (TypeError, ValueError):
                digest = None
            if key in h._requests:
                old, response = h._requests[key]
                if digest is None or old != digest or not isinstance(response, dict):
                    raise ActionRejected('REQUEST_CONFLICT')
                return copy.deepcopy(response)
            if not isinstance(capability_id, str) or capability_id not in CAPS:
                raise ActionRejected('UNKNOWN_CAPABILITY')
            if self._role == 'agent' and CAPS[capability_id]['visibility'] != 'agent':
                raise ActionRejected('ACCESS_DENIED')
            if not isinstance(arguments, dict):
                raise ActionRejected('INVALID_ARGUMENT')
            validate(CAPS[capability_id]['input_schema'], arguments)
            self._guard(capability_id, arguments)
            result = h.invoke(capability_id, **arguments)
            if self._role == 'agent':
                if capability_id == 'records.list':
                    result = dict(records=[r for r in result['records'] if not self._foreign(r)])
                elif capability_id == 'log.read':
                    result = dict(entries=[e for e in result['entries'] if not self._foreign(e['record_id'])])
                elif capability_id == 'events.read':
                    result = dict(events=[{k: v for k, v in e.items() if k != '_rid'} for e in h.events
                                          if e['time_s'] >= arguments['since_s'] and not self._foreign(e.get('_rid')) and e['kind'] != 'fault'], dropped=h.events_dropped)
            operation = result.get('operation_id') if capability_id in ASYNC else None
            if operation:
                h._owners[operation] = self._principal
                rid = h.operations[operation].get('record_id')
                if rid:
                    h._owners[rid] = self._principal
            response = dict(protocol_version=PROTOCOL_VERSION, instance_id=instance_id, request_id=request_id, operation_id=operation,
                            status='accepted' if operation else 'succeeded', state_revision=h.revision, clock={'domain': 'simulation', 'time_s': h.time_s},
                            result=copy.deepcopy(result), observations={}, error=None)
            h._requests[key] = (digest, copy.deepcopy(response))
            return response
        except ActionRejected as exc:
            response = dict(protocol_version=PROTOCOL_VERSION, instance_id=instance_id, request_id=request_id, operation_id=None,
                            status='unsupported' if str(exc) == 'UNSUPPORTED' else 'rejected', state_revision=h.revision,
                            clock={'domain': 'simulation', 'time_s': h.time_s}, result={}, observations={},
                            error=dict(code=str(exc), message=ERROR_ZH.get(str(exc), str(exc)), retryable=False, side_effects='none'))
            if key is not None and digest is not None and key not in h._requests:
                h._requests[key] = (digest, copy.deepcopy(response))
            return response

    def write(self, *, instance_id, state_id, value, request_id):
        self._target(instance_id)
        raise ActionRejected('ACCESS_DENIED')

    def poll(self, operation_id):
        h = self._host
        if not isinstance(operation_id, str):
            raise ActionRejected('INVALID_ARGUMENT')
        op = h.operations.get(operation_id)
        if op is None:
            raise ActionRejected('UNKNOWN_OPERATION')
        if h._owners.get(operation_id) != self._principal:
            raise ActionRejected('ACCESS_DENIED')
        res = h._out(h.records[op['record_id']]) if op.get('record_id') in h.records else dict(phase=op['phase'])
        return dict(operation_id=operation_id, status='succeeded' if op['phase'] == 'completed' else op['phase'], result=res)

    def cancel(self, operation_id, request_id):
        h = self._host
        if not isinstance(request_id, str) or not request_id:
            raise ActionRejected('INVALID_ARGUMENT')
        key = (self._principal, request_id)
        digest = json.dumps(['cancel', operation_id]) if isinstance(operation_id, str) else None
        if key in h._requests:
            old, response = h._requests[key]
            if digest is None or old != digest:
                raise ActionRejected('REQUEST_CONFLICT')
            if isinstance(response, str):
                raise ActionRejected(response)
            return copy.deepcopy(response)
        try:
            self.poll(operation_id)
            op = h.operations[operation_id]
            if op['phase'] != 'running':
                raise ActionRejected('NOT_RUNNING')
            h._cancel_op(op)
        except ActionRejected as exc:
            if digest is not None:
                h._requests[key] = (digest, str(exc))
            raise
        h.revision += 1
        response = dict(protocol_version=PROTOCOL_VERSION, instance_id=h.instance_id, request_id=request_id, operation_id=operation_id, status='succeeded',
                        state_revision=h.revision, clock={'domain': 'simulation', 'time_s': h.time_s}, result=dict(phase=op['phase']), observations={}, error=None)
        h._requests[key] = (digest, copy.deepcopy(response))
        return response


ERROR_ZH = {
    'INVALID_ARGUMENT': '参数不符合输入模式或取值范围', 'REQUEST_CONFLICT': '同一request_id对应不同的调用', 'UNKNOWN_INSTANCE': '实例不存在',
    'UNKNOWN_CAPABILITY': '能力不存在', 'UNSUPPORTED': '本配置不支持该能力', 'ACCESS_DENIED': '无权访问（真值槽、另一个agent的记录或操作）',
    'PLANT_REQUIRED': '尚未声明实验室条件', 'SEED_FIXED': '噪声种子只能在首次声明时设定', 'MAINS_OUT_OF_RANGE': '电源电压超出100–240 VAC ±10 %',
    'POWER_OFF': '仪器未通电', 'OPERATION_RUNNING': '有运行中的灌注/洗板/清洁', 'UNKNOWN_LIQUID': '洗液模板库中没有该液体',
    'PLATE_EXISTS': '板ID已存在', 'TOO_MANY_PLATES': '板数量超过上限（20）', 'UNKNOWN_PLATE_TYPE': '板模板库中没有该板型',
    'PLATE_INCOMPATIBLE': '单96管洗头只能处理96孔板（384孔板需双洗头或192管洗头）', 'WELL_OVERFILLED': '初始体积超过孔体积', 'UNKNOWN_PLATE': '板不存在',
    'PLATE_NOT_ON_BENCH': '板不在实验台上', 'CARRIER_OCCUPIED': '载板上已有板', 'CARRIER_NOT_HOME': '载板不在初始位或洗头未停放',
    'PLATE_NOT_ON_CARRIER': '该板不在载板上', 'PLATE_ON_CARRIER': '灌注前请取下载板上的板（灌注槽在载板下方）', 'NO_PLATE': '载板上没有板',
    'WASTE_FULL': '废液瓶满（液位传感器）', 'BUFFER_EMPTY': '所选供液瓶无液（液体传感器）', 'VACUUM_LOW': '真空不足（泵未接或泄漏）',
    'CELL_WASH_OPTION_ABSENT': '细胞洗涤流速1–2与CW行进速度需要细胞洗涤选件（低流量管路）', 'VOLUME_NOT_IN_1UL_STEPS': '分配体积须为1 µL的整数倍',
    'RECORDS_FULL': '记录数已达上限（200）', 'UNKNOWN_RECORD': '记录不存在', 'UNKNOWN_OPERATION': '操作不存在', 'NOT_RUNNING': '操作已结束',
}
_PLATE = ['plate.on_carrier', 'plate.bench_ids', 'truth.residual_m3']
_OPS = ['operation.kind', 'operation.progress', 'program.phase', 'display.cycles_remaining', 'display.time_remaining_s']
_MOVE = ['carrier.x_m', 'carrier.at_home', 'manifold.z_m', 'manifold.tip_height_m']
_FLUID = ['buffer.selected', 'sensor.fluid_ok', 'truth.line_composition'] + [f'buffer.{b.lower()}.volume_m3' for b in BOTTLES]
_VAC = ['sensor.vacuum_ok']
EFFECTS = {
    'plant.configure': ['plant.configured', 'power.enabled', 'sensor.vacuum_ok', 'sensor.waste_full'] + _OPS,
    'power.set': ['power.enabled', 'sensor.vacuum_ok', 'display.cycles_remaining', 'display.time_remaining_s'] + _OPS + _MOVE,
    'power.press_switch': ['power.enabled', 'sensor.vacuum_ok', 'display.cycles_remaining', 'display.time_remaining_s'] + _OPS + _MOVE,
    'buffer.load': [f'buffer.{b.lower()}.liquid' for b in BOTTLES] + [f'buffer.{b.lower()}.volume_m3' for b in BOTTLES] + ['sensor.fluid_ok'],
    'waste.empty': ['sensor.waste_full', 'truth.waste_volume_m3'], 'plate.provide': ['plate.bench_ids'], 'plate.load': _PLATE, 'plate.unload': _PLATE,
    'prime.run': _OPS + _MOVE + ['buffer.selected'], 'wash.run': _OPS + _MOVE + ['buffer.selected'], 'maintenance.clean_tubes': _OPS + ['program.phase'],
    'fault.inject': ['truth.faults', 'truth.clogged_dispense', 'truth.clogged_aspirate', 'sensor.vacuum_ok', 'sensor.waste_full', 'sensor.fluid_ok', 'power.enabled',
                     'display.cycles_remaining', 'display.time_remaining_s', 'result.last_record_id'] + _OPS + _MOVE,
    'sim.step': ['clock.time_s', 'truth.idle_s'],
}
_RUN_STEP = _OPS + _MOVE + _FLUID + _VAC + ['result.last_record_id', 'sensor.waste_full', 'sensor.flow_ok', 'truth.waste_volume_m3', 'truth.residual_m3',
                                           'truth.clogged_dispense', 'truth.clogged_aspirate', 'truth.idle_s', 'truth.line_composition']
ON_STEP = {'prime.run': _RUN_STEP, 'wash.run': _RUN_STEP, 'maintenance.clean_tubes': _OPS + ['result.last_record_id', 'truth.clogged_dispense', 'truth.clogged_aspirate',
                                                                                           'truth.idle_s'],
           'fault.inject': _RUN_STEP, 'plant.configure': _RUN_STEP, 'power.set': _RUN_STEP, 'power.press_switch': _RUN_STEP,   # stopping a run stops its later phases
           'sim.step': _RUN_STEP}
ON_STEP_ZH = {'prime.run': '按阶段推进：切阀、分配到灌注槽（管路组成更新、废液增加）、停放', 'wash.run': '按阶段推进：载板移动、抽吸、分配、浸泡、摇动、最终抽吸、回初始位',
              'maintenance.clean_tubes': '10 min后解除全部堵针', 'fault.inject': '断电或真空泄漏使运行失败（真空泄漏时洗头停放、载板回初始位，断电时停在原处），之后的阶段（抽吸、分配、废液、管路组成、显示）不再推进', 'plant.configure': '电源电压超范围断电时进行中的运行失败，之后的阶段不再推进', 'power.set': '关机使进行中的运行失败，之后的阶段不再推进', 'power.press_switch': '关机使进行中的运行失败，之后的阶段不再推进', 'sim.step': '时钟推进；进行中的操作按阶段完成；空闲时管路干涸'}
PRECONDITIONS = {
    'plant.configure': ('噪声种子只能在首次声明时设定；运行中不能改真空泵与细胞洗涤选件', ['plant.configured', 'operation.kind']),
    'power.set': ('已声明实验室条件；开机时电源电压在100–240 VAC ±10 %', ['plant.configured', 'power.enabled']),
    'power.press_switch': ('已声明实验室条件；断电时按下为开机（电压须在范围内），通电时按下为关机（进行中的运行失败）', ['plant.configured', 'power.enabled']),
    'buffer.load': ('已声明实验室条件；液体在洗液模板库中；不能更换运行中正在用的瓶', ['plant.configured', 'operation.kind', 'buffer.selected']),
    'waste.empty': ('已声明实验室条件；无运行', ['plant.configured', 'operation.kind']),
    'plate.provide': ('已声明实验室条件；板型在模板库中且为96孔；初始体积不超过孔体积', ['plant.configured', 'plate.bench_ids']),
    'plate.load': ('板在实验台上、载板空、载板在初始位且洗头停放、无运行', ['plate.bench_ids', 'plate.on_carrier', 'carrier.at_home', 'operation.kind']),
    'plate.unload': ('板在载板上、载板在初始位、无运行', ['plate.on_carrier', 'carrier.at_home', 'operation.kind']),
    'prime.run': ('通电、无运行、载板空且在初始位（灌注槽露出）、所选瓶有液、废液未满、真空正常', ['power.enabled', 'operation.kind', 'plate.on_carrier', 'carrier.at_home',
                                                                                  'sensor.fluid_ok', 'sensor.waste_full', 'sensor.vacuum_ok']),
    'wash.run': ('通电、无运行、载板上有板且在初始位、所选瓶有液、废液未满、真空正常；细胞洗涤档位需选件；体积为1 µL整数倍，真空延迟不大于分配体积',
                 ['power.enabled', 'operation.kind', 'plate.on_carrier', 'carrier.at_home', 'sensor.fluid_ok', 'sensor.waste_full', 'sensor.vacuum_ok']),
    'maintenance.clean_tubes': ('已声明实验室条件；无运行', ['plant.configured', 'operation.kind']),
}
_COMMON_ERRORS = ['INVALID_ARGUMENT', 'REQUEST_CONFLICT', 'UNKNOWN_INSTANCE']
_IDLE = ['PLANT_REQUIRED', 'OPERATION_RUNNING']
CAP_ERRORS = {
    'plant.configure': ['SEED_FIXED', 'OPERATION_RUNNING'], 'power.set': ['PLANT_REQUIRED', 'MAINS_OUT_OF_RANGE'], 'power.press_switch': ['PLANT_REQUIRED', 'MAINS_OUT_OF_RANGE'],
    'buffer.load': ['PLANT_REQUIRED', 'UNKNOWN_LIQUID', 'OPERATION_RUNNING'], 'waste.empty': ['PLANT_REQUIRED', 'OPERATION_RUNNING'],
    'plate.provide': ['PLANT_REQUIRED', 'PLATE_EXISTS', 'TOO_MANY_PLATES', 'UNKNOWN_PLATE_TYPE', 'PLATE_INCOMPATIBLE', 'UNKNOWN_LIQUID', 'WELL_OVERFILLED'],
    'plate.load': _IDLE + ['UNKNOWN_PLATE', 'PLATE_NOT_ON_BENCH', 'CARRIER_OCCUPIED', 'CARRIER_NOT_HOME'], 'plate.unload': _IDLE + ['PLATE_NOT_ON_CARRIER', 'CARRIER_NOT_HOME'],
    'prime.run': _IDLE + ['POWER_OFF', 'CARRIER_NOT_HOME', 'PLATE_ON_CARRIER', 'WASTE_FULL', 'BUFFER_EMPTY', 'VACUUM_LOW', 'RECORDS_FULL'],
    'wash.run': _IDLE + ['POWER_OFF', 'NO_PLATE', 'CARRIER_NOT_HOME', 'CELL_WASH_OPTION_ABSENT', 'VOLUME_NOT_IN_1UL_STEPS', 'WASTE_FULL', 'BUFFER_EMPTY', 'VACUUM_LOW', 'RECORDS_FULL'],
    'maintenance.clean_tubes': _IDLE + ['RECORDS_FULL'], 'log.read': [], 'measurement.result': ['UNKNOWN_RECORD'], 'records.list': [], 'events.read': [], 'ledger.read': [],
    'plate.inspect': ['UNKNOWN_PLATE'], 'provenance.read': [], 'fault.inject': ['PLANT_REQUIRED'], 'sim.step': [],
}
_PARTIAL = {'WASTE_FULL', 'BUFFER_EMPTY', 'VACUUM_LOW'}
CAP_ERROR_ZH = {('wash.run', 'WASTE_FULL'): '启动时废液满；或运行中废液满（记录failed，已完成的循环效果保留）',
                ('wash.run', 'BUFFER_EMPTY'): '启动时供液瓶无液；或运行中瓶空（记录failed，已完成的循环效果保留）',
                ('wash.run', 'VACUUM_LOW'): '启动时真空不足；或运行中真空泄漏（记录failed，孔内保留液体）',
                ('prime.run', 'WASTE_FULL'): '废液满（启动或运行中）', ('prime.run', 'BUFFER_EMPTY'): '供液瓶无液（启动或运行中）', ('prime.run', 'VACUUM_LOW'): '真空不足'}
MODEL_NONE_ZH = {
    'plant.configure': '只声明宿主实验室条件与外部附件，没有可见模型变化', 'buffer.load': '供液瓶是外部附件，场景中没有瓶子几何', 'waste.empty': '废液瓶是外部附件',
    'plate.provide': '只在过程层实验台上增加板；场景没有板几何（载板板位为接口点）', 'plate.load': '板只在过程层（场景载板板位为接口点）', 'plate.unload': '同上',
    'maintenance.clean_tubes': '人工/机器人维护抽象，没有可见模型变化', 'log.read': '只读日志', 'measurement.result': '只读记录', 'records.list': '只读列表',
    'events.read': '只读事件', 'ledger.read': '只读账目（evaluator）', 'plate.inspect': '只读真值（evaluator）', 'provenance.read': '只读来源记录',
    'fault.inject': '评估者故障注入', 'sim.step': '推进时钟',
}
SCENE_MIRRORED = {'carrier.x_m', 'manifold.z_m', 'power.enabled', 'display.cycles_remaining', 'display.time_remaining_s'}


def _model_effects(k, un):
    if un:
        return dict(mode='none', description_zh='不支持：不实现，没有模型变化', binding_ids=[])
    seen = sorted((set(EFFECTS.get(k, [])) | set(ON_STEP.get(k, []))) & SCENE_MIRRORED)
    if seen:
        return dict(mode='host_required', binding_ids=[],
                    description_zh='状态经宿主桥（Matterix）镜像到场景关节或屏幕数字（' + '、'.join(seen) + '；绑定见合同model_bindings），运行层本身不驱动模型'
                                   + ('；' + MODEL_NONE_ZH[k] if k in MODEL_NONE_ZH else ''))
    return dict(mode='none', description_zh=MODEL_NONE_ZH.get(k, '没有镜像到场景的状态变化'), binding_ids=[])


EXEC_TEXT = {
    'prime.run': ('操作结束：切阀0.4 s、按流速分配给定体积到灌注槽（96管合计）、停放', '失败时（瓶空、废液满、真空不足、断电）已分配部分计入管路组成与废液。'),
    'wash.run': ('操作结束：按程序逐阶段（载板移动、抽吸、分配、底洗、浸泡、摇动、最终抽吸）完成并回初始位，写wash记录',
                 '取消时洗头停放、载板回初始位；故障时记录failed：真空、废液或供液故障时洗头停放、载板回初始位，断电时洗头与载板都停在原处（再次通电自检后回位）；已完成阶段的孔内效果保留。'),
    'maintenance.clean_tubes': ('操作结束：10 min后解除全部堵针', '取消时堵针保持。'),
}
RESOURCES = {'prime.run': ['manifold', 'buffer_valves', 'vacuum', 'host_clock'], 'wash.run': ['manifold', 'plate_carrier', 'buffer_valves', 'vacuum', 'host_clock'],
             'maintenance.clean_tubes': ['manifold', 'host_clock'], 'plate.load': ['plate_carrier'], 'plate.unload': ['plate_carrier'], 'buffer.load': ['supply_bottles'],
             'waste.empty': ['waste_bottles'], 'power.set': ['mains'], 'power.press_switch': ['mains'], 'plant.configure': ['room', 'mains', 'accessories'],
             'plate.provide': ['bench'], 'fault.inject': ['evaluator'], 'sim.step': ['host_clock']}
OBSERVES = {
    'power.set': ['power.enabled'], 'power.press_switch': ['power.enabled'], 'buffer.load': ['sensor.fluid_ok'], 'waste.empty': ['sensor.waste_full'],
    'plate.load': ['plate.on_carrier'], 'plate.unload': ['plate.bench_ids'], 'prime.run': ['operation.kind', 'program.phase', 'result.last_record_id'],
    'wash.run': ['operation.kind', 'program.phase', 'display.cycles_remaining', 'display.time_remaining_s', 'carrier.x_m', 'manifold.z_m', 'result.last_record_id'],
    'maintenance.clean_tubes': ['operation.kind', 'result.last_record_id'],
}
_WB = 'user_workbook'
_GSG = 'manufacturer_getting_started_guide'
_EL = 'manufacturer_el406_manual'
_SLAS = 'ansi_slas_standards'
_ARB = 'arbor_plate_dimensions'
_VDI = 'vdi_ppds'
_LAL = 'laliberte2009'
_SIM = 'simulation_assumption'
CAP_SOURCES = {k: [_SIM] for k in CAPS}
CAP_SOURCES.update({
    'power.set': [_GSG], 'power.press_switch': [_GSG], 'buffer.load': [_GSG, _VDI, _LAL], 'waste.empty': [_GSG], 'plate.provide': [_ARB, _SLAS, _WB, _SIM],
    'plate.load': [_GSG, _SLAS], 'plate.unload': [_GSG], 'prime.run': [_GSG, _SIM], 'wash.run': [_WB, _GSG, _EL, _VDI, _LAL, _SLAS, _ARB, _SIM],
    'maintenance.clean_tubes': [_GSG, _SIM], 'provenance.read': [_VDI, _LAL, _ARB]})
CAP_SOURCES.update({k: [_GSG] for k in UNSUPPORTED})
PARAM_ZH = {'room_k': '实验室室温（洗液物性按此取值）', 'mains_voltage_v': '供电电压', 'noise_seed': '噪声随机数种子（只在首次声明时生效）',
            'vacuum_pump': '真空泵接在附件插座上', 'cell_wash_installed': '细胞洗涤选件（低流量管路，流速1–2与CW行进速度）', 'waste_capacity_m3': '废液瓶总容量'}
_DEFAULT_LIMIT = '过程层实现；机器人与硬件层未实现；整体模型边界见mechanisms.washer_405ts.limitations。'
CAP_LIMITS = {
    'wash.run': ['残余体积按孔底液层（g²/(g+g0)）、孔底液膜、底角液环与Landau–Levich壁面液膜计算，参数按手册两点标定（默认29步≤2 µL；50步约100 µL，模型约88 µL：圆柱孔在该高度的液层上限约90 µL）；溢流无传感器，只作可见警告；'
                 '流速档位对应的每孔流量、行进速度（2、3档为EL406手册值，其余为工程值）、分配CV分解、射流混合与剪切、解吸速率为工程值；'
                 '细胞脱落、堵针、滴落与溢流污染为工程模型，未经实验标定。',
                 '洗头X/Y偏移、扇区洗、384孔、双洗头与底部抽滤（Vac）未实现；真空泵抽速与废液管路压降未建模（真空只有好/坏两态）。'],
    'prime.run': ['管路死体积110 mL按两级串联全混釜处理（工程模型；300 mL灌注后纯度约97 %，与厂家“≥95 %”“三倍死体积保证纯度”一致）；低流量管路（细胞洗涤选件）的单独灌注未区分。'],
    'plate.provide': ['板内“游离物/非特异/特异结合物”为通用ELISA代理量（mol），不对应某一具体抗体或底物；细胞以每孔数量与黏附强度（弱/强）表示。'],
    'buffer.load': ['洗液物性取洗液模板库：纯液体为VDI PPDS关联式，缓冲液密度/黏度为Laliberté (2009)模型，表面张力对稀盐溶液取水、对吐温洗液取36 mN/m（假设）；'
                    '有机溶剂与管路材料的化学相容性未检查。'],
    'maintenance.clean_tubes': ['用清洁针逐根疏通为人工/机器人维护抽象（10 min）；超声自动清洗为选件（不支持）。'],
}
LIMITATIONS = [
    '选定配置：405 TS单96管洗头（只处理96孔板）、内置四路缓冲切换（A–D）、标准载板；外部真空泵、供液瓶与废液瓶为附件（场景不建）；'
    '细胞洗涤低流量管路为选件（plant.cell_wash_installed，默认未装）；真空过滤、磁珠分离、Verify堵针检测、超声清洗与BioStack不支持。',
    '孔几何取板模板库（SBS/ANSI-SLAS标准与经销商板尺寸表；孔底高度为推导值），孔底高度在板内按SLAS-6变化量随机分布。',
    '板、液体与细胞在场景中没有几何；放板、加液与倒废液是宿主/机器人抽象，没有接触验证。',
    '没有真实405 TS与LHC软件的硬件接口。',
]


def _sampling_text(k):
    if k in ('clock.time_s', 'operation.progress', 'display.time_remaining_s', 'truth.idle_s'):
        return 'Instantaneous value at the read time; operation phases complete at their scheduled times inside step().'
    return 'Changes when a command is accepted or at a phase event of an operation; no other time dynamics.'


def _errors_of(k):
    if k in UNSUPPORTED:
        return ['UNSUPPORTED'] + _COMMON_ERRORS
    extra = ['ACCESS_DENIED'] if (k in _CONTROL_GUARDED or k in _DATA_GUARDED or CAPS[k]['visibility'] != 'agent') else []
    return list(dict.fromkeys(CAP_ERRORS.get(k, []) + _COMMON_ERRORS + extra))


def _foreign_text(k):
    return '；另一个agent的操作进行中时，agent调用被拒（ACCESS_DENIED；未通电时开机除外）' if k in _CONTROL_GUARDED else ''


def export_biotek_405ts_contract_metadata():
    slots, caps = {}, {}
    for k, v in SLOTS.items():
        slots[k] = dict(meaning_zh=v['meaning'], component_ids=[v['component']], value_schema=copy.deepcopy(v['schema']), unit=v['unit'],
                        reference_conditions=v['reference'], origin=v['origin'], visibility=v['visibility'], access='read_only',
                        read_binding=dict(backend='host', artifact_id='runtime', accessor=f'Biotek405tsSession.read(state_ids=[{k!r}])'), write_via=None,
                        sampling=dict(mode='instant', time_domain='simulation', window_description=_sampling_text(k)), source_ids=['simulation_assumption'])
    for k, v in CAPS.items():
        un = k in UNSUPPORTED
        async_ = k in ASYNC
        c = {key: copy.deepcopy(v[key]) for key in ['input_schema', 'output_schema', 'component_ids', 'function_zh', 'kind', 'visibility', 'example_arguments']}
        c.update(availability='unsupported' if un else 'implemented',
                 implementation={layer: 'absent' if un or layer in ('scene', 'robot', 'hardware') else 'implemented' for layer in ['process', 'scene', 'robot', 'hardware', 'host']},
                 binding=None if un else dict(backend='host', artifact_id='runtime', entrypoint='asset_runtime.biotek_405ts.Biotek405tsSession.invoke',
                                              invocation=f'session.invoke(instance_id="washer_01",capability_id={k!r},arguments={v["example_arguments"]!r},request_id="unique")'),
                 preconditions=[] if un or k not in PRECONDITIONS else [dict(description_zh=PRECONDITIONS[k][0] + _foreign_text(k) + '；被拒时状态不变。',
                                                                              state_ids=list(PRECONDITIONS[k][1]) + (['operation.started_by_other_agent'] if k in _CONTROL_GUARDED else []),
                                                                              enforced_by='runtime', check='Biotek405tsRuntime.invoke / Biotek405tsSession._guard')],
                 state_effects=[dict(state_id=s, timing='on_step' if k == 'sim.step' else 'immediate', description_zh='设定或立即改变的量；运行阶段只随step推进')
                                for s in dict.fromkeys(EFFECTS.get(k, []))],
                 observes=[] if un else list(OBSERVES.get(k, [])), model_effects=_model_effects(k, un),
                 execution=dict(mode='asynchronous' if async_ else 'synchronous', time_domain='simulation',
                                completion_criterion=EXEC_TEXT[k][0] if k in EXEC_TEXT else '原子设定、配置或查询',
                                completion_state_ids=['operation.kind', 'result.last_record_id'] if async_ else [],
                                timeout_s=None, cancellation='supported' if async_ else 'unsupported', retry='same_request_id', resources=list(RESOURCES.get(k, [])),
                                partial_failure_zh=EXEC_TEXT[k][1] if k in EXEC_TEXT else ('不支持：不执行，状态不变' if un else '同步原子操作：成功时一次写入全部效果，被拒时状态不变')),
                 errors=[dict(code=e, meaning_zh=CAP_ERROR_ZH.get((k, e), ERROR_ZH.get(e, e)), side_effects='partial' if async_ and e in _PARTIAL else 'none') for e in _errors_of(k)],
                 verification=[], limitations=[UNSUPPORTED[k]] if un else list(CAP_LIMITS.get(k, [_DEFAULT_LIMIT])), source_ids=list(CAP_SOURCES[k]))
        if k in ON_STEP:
            c['state_effects'] += [dict(state_id=s, timing='on_step', description_zh=ON_STEP_ZH.get(k, '随step演化')) for s in dict.fromkeys(ON_STEP[k])
                                   if not (k == 'sim.step' and s in EFFECTS.get(k, []))]
        caps[k] = c
    params = {}
    for name, (val, unit, meaning, src) in {
            'volume_range_m3': (list(VOLUME_RANGE_M3), 'm3', '96管洗头分配体积50–3000 µL/孔（厂家；调研表已核验）', [_WB, _GSG]),
            'volume_step_m3': (VOLUME_STEP_M3, 'm3', '体积步进1 µL（调研表）', [_WB]), 'cycles_range': (list(CYCLES_RANGE), '1', '清洗1–250循环（调研表）', [_WB]),
            'residual_spec_m3': (RESIDUAL_SPEC_M3, 'm3', '三循环后平均残余≤2 µL/孔（300 µL水+0.1 %吐温-20，Costar平底板，厂家）', [_GSG, _WB]),
            'cv_spec': (CV_SPEC, '1', '300 µL/孔分配精密度≤3 % CV（厂家）', [_GSG, _WB]), 'z_step_m': (Z_STEP_M, 'm', '洗头Z步长0.127 mm（EL406手册；50步≈6.35 mm）', [_EL, _GSG]),
            'aspirate_z_default': (ASP_Z_DEFAULT, '1', '96孔板默认抽吸高度29步（厂家）', [_GSG]), 'dispense_z_default': (DISP_Z_DEFAULT, '1', '96孔板默认分配高度121步（厂家）', [_GSG]),
            'aspirate_delay_max_s': (ASP_DELAY_MAX_S, 's', '抽吸延时0–5000 ms（厂家）', [_GSG]), 'vacuum_delay_range_m3': (list(VAC_DELAY_M3), 'm3', '真空延迟10–350 µL/孔（厂家）', [_GSG]),
            'shake_soak_max_s': (SHAKE_SOAK_MAX_S, 's', '摇动/浸泡1 s–1 h（厂家）', [_GSG]), 'home_after_s': (HOME_AFTER_S, 's', '浸泡+摇动超过1 min载板回初始位、真空泵关（厂家）', [_GSG]),
            'dead_volume_m3': (DEAD_VOLUME_M3, 'm3', '死体积108 mL＋缓冲切换管路2 mL（厂家）', [_GSG]), 'prime_for_change_m3': (PRIME_FOR_CHANGE_M3, 'm3', '换液推荐灌注300 mL（纯度≥95 %，厂家）', [_GSG]),
            'travel_speed_m_s': ({k: v for k, v in TRAVEL_SPEED.items()}, 'm/s', '抽吸行进速度：2档3.4 mm/s、3档4.0 mm/s（EL406手册，同系列洗头）', [_EL]),
            'accessory_outlet_a': (ACCESSORY_OUTLET_A, 'A', '真空泵附件插座≤5 A（厂家）', [_GSG])}.items():
        params[name] = dict(value=val, unit=unit, meaning_zh=meaning, evidence='documented', source_ids=list(src), uncertainty=None)
    for name, (val, unit, meaning) in {
            'flow_rate_m3_s': ({str(k): v for k, v in FLOW_RATE_M3_S.items()}, 'm3/s', '分配流速档位1–11对应的每孔流量（按“300 µL三循环≤30 s”与默认7档反推的工程值）'),
            'travel_rates': ({k: v[0] for k, v in TRAVEL.items()}, 'm/s', '抽吸行进速度（除2、3档外为工程值）'),
            'travel_disturbance': ({k: v[1] for k, v in TRAVEL.items()}, '1', '行进速度档对细胞层的扰动系数（CW档较小）'),
            'line_tanks': (LINE_TANKS, '1', '管路按串联全混釜数（标定到厂家300 mL灌注≥95 %）'), 'park_tip_m': (PARK_TIP_M, 'm', '洗头停放时抽吸管尖高于板位'), 'disp_tip_above_asp_m': (DISP_TIP_ABOVE_ASP_M, 'm', '分配管比抽吸管短'),
            'carrier_travel_m': (CARRIER_TRAVEL_M, 'm', '载板行程'), 'carrier_move_s': (CARRIER_MOVE_S, 's', '载板移动时间'), 'lift_speed_m_s': (LIFT_SPEED_M_S, 'm/s', '洗头快速升降速度'),
            'valve_s': (VALVE_S, 's', '切阀时间'), 'tube_inner_r_m': (TUBE_INNER_R_M, 'm', '分配管内半径'), 'low_flow_r_m': (LOW_FLOW_R_M, 'm', '低流量出口等效半径'),
            'g0_m': (G0_M, 'm', '孔底液层排空长度（按50步≈100 µL标定）'), 'delta0_m': (DELTA0_M, 'm', '管尖到底时残留的孔底液膜厚度'),
            'fillet_factor': (FILLET_FACTOR, '1', '底角液环半径/毛细长度'), 'residual_scatter': (RESIDUAL_SCATTER, '1', '单孔残余的对数正态离散'),
            'wbe_scatter_m': (WBE_SCATTER_M, 'm', '板内孔底高度离散'), 'cv_repeat': (CV_REPEAT, '1', '分配逐次离散'), 'cv_tube': (CV_TUBE, '1', '管间固定偏差'),
            'cv_abs_m3': (CV_ABS_M3, 'm3', '分配绝对离散'), 'mix_jet_m_s': (MIX_JET_M_S, 'm/s', '射流混合特征速度'), 'mix_min': (MIX_MIN, '1', '慢速注液对残液的最低对流混合效率'), 'shake_tau_s': (dict(SHAKE_TAU), 's', '摇动混合时间常数'),
            'k_off_ns': (K_OFF_NS, '1/s', '非特异吸附解吸速率（无表面活性剂）'), 'k_off_ns_surf': (K_OFF_NS_SURF, '1/s', '非特异吸附解吸速率（含吐温）'),
            'k_off_spec': (K_OFF_SPEC, '1/s', '特异结合解离速率'), 'tau_cell_pa': (TAU_CELL_PA, 'Pa', '弱贴壁细胞e倍脱落的壁面剪切（强贴壁×4）'),
            'cell_layer_clear_m': (CELL_LAYER_CLEAR_M, 'm', '管尖低于此间隙时刮擦细胞层'), 'dry_dwell_s': (DRY_DWELL_S, 's', '近干孔损伤细胞的停留时间'),
            'clog_tau_s': (CLOG_TAU_S, 's', '1 %盐洗液在空闲洗头中的堵针时间尺度'), 'meniscus_m': (MENISCUS_M, 'm', '液面可高出孔口的高度'),
            'idle_w': (IDLE_W, 'W', '待机电功率'), 'active_w': (ACTIVE_W, 'W', '运行电功率'), 'drop_m3': (DROP_M3, 'm3', '管尖外壁带液'),
            'drip_p_per_s': (DRIP_P_PER_S, '1/s', '板停在洗头下时每管每秒滴落概率'), 'drops_per_tip': (DROPS_PER_TIP, '1', '每次分配后每个管尖最多可滴落的液滴数')}.items():
        params[name] = dict(value=val, unit=unit, meaning_zh=meaning, evidence='assumption', source_ids=[_SIM], uncertainty='工程值')
    mechanism = dict(
        component_ids=['manifold', 'manifold_tubes', 'plate_carrier', 'prime_trough', 'buffer_inlets', 'rear_connectors', 'touch_screen', 'switch_power'],
        state_ids=list(slots), implementation='lumped',
        meaning_zh='405 TS洗板：供液瓶–缓冲阀–管路（死体积）–96管洗头的分配与真空抽吸，孔内残余、清洗效率、细胞脱落、堵针、溢流/滴落污染与传感器报警',
        equations=['fluid path: LINE_TANKS = 2 stirred tanks in series of V_dead / 2 each (V_dead = 110 mL): purity after priming V = 1 - exp(-2V/V_dead)(1 + 2V/V_dead)',
                   'residual = A g^2/(g + g0) + A delta0 + 2 pi r (1 - pi/4) (0.1 l_c)^2 + 2 pi r (V/A) 0.94 l_c Ca^(2/3); g = z_tip - z_well_bottom, Ca = mu U / sigma, l_c = sqrt(sigma / (rho g))',
                   'dispensed per tube: v = V_set f_clog (1 + b_tube + N(0, sqrt(cv_rep^2 + (dv/V_set)^2)))',
                   'two-compartment well: after a dispense u = (1 - m_jet) V0/(V0 + v) of the species sit in the bottom layer L = V0, m_jet = 1 - (1 - m_min) exp(-v_j / 0.6 m/s); soak / shake mix u -> 0',
                   'soluble species after aspiration: n <- n f, f = u res/L + (1 - u) res/V (res <= L) or u + (1 - u) res/V ; desorption: dn_ns = n_ns (1 - exp(-k_off t_contact))',
                   'cell detachment by the jet: N <- N exp(-(tau_w / tau_c)^2), tau_w = 0.01 rho v_j^2 exp(-h / 2 mm), v_j = Q / (pi r_tube^2)',
                   'species leaving at the top while the jet runs (skim or spill): n <- n (u + (1 - u) exp(-V_excess / V_cap)); level cap with the vacuum on and a free aspirate tube: V <= A (z_tip - z_bottom) (tip below the rim) or V_well + A h_men/2 (z_tip - z_rim)/h_men (tip up to h_men = 1.5 mm above); otherwise V > V_well + A h_men / 2 overflows to the four neighbours',
                   'drips from the parked manifold: Poisson per tube (rate p_drip), at most DROPS_PER_TIP drops of 0.25 uL of clean line buffer after each dispense, prime or bottom wash; a full well runs over'],
        parameters=params, source_ids=[_SIM, _WB, _GSG, _EL, _VDI, _LAL, _SLAS, _ARB], limitations=list(LIMITATIONS))
    import importlib.util
    spec = importlib.util.spec_from_file_location('_405ts_local_annotations', ROOT / 'scripts/asset_runtime/_annotations.py')
    ann = importlib.util.module_from_spec(spec)
    spec.loader.exec_module(ann)
    ann.KEY_ZH.update({k: v['meaning'] for k, v in SLOTS.items()})
    ann.PARAM_ZH.update(PARAM_ZH)
    ann.annotate_export('biotek_405ts', caps, slots, {'washer_405ts': mechanism}, inputs={'plant.configure': (PLANT, PLANT_EXAMPLE)})
    for k, v in SLOTS.items():
        slots[k]['meaning_zh'] = v['meaning']
        if v['reference']:
            slots[k]['reference_conditions'] = v['reference']
    return dict(asset_type_id=ASSET_TYPE_ID, catalog_id=CATALOG_ID, capabilities=caps, state_slots=slots, mechanisms={'washer_405ts': mechanism})