{"record_kind": "run_header", "run_id": "pearl-manuscript-s8-20260916", "seq": 1, "trace_sequence": 1, "timestamp": "2026-09-16T10:24:12+00:00", "logged_at": "2026-09-16T10:24:12.350000+00:00", "source_agent": null, "scenario_id": "S8", "provenance": "manuscript-aligned-case-log-v1", "not_wet_lab": false, "world_contract": "manuscript-case-S8", "config": {"communication_mode": "typed_events", "ir_schema_version": 4}, "user_command": "Autonomously characterize the HRP–TMB assay: identify its linear working range and estimate Km and kcat from time-resolved measurements.", "sources": [{"well": "A1", "label": "Enzyme stock (HRP), 30 nM", "volume_ul": 2000, "concentration_nM": 30.0, "concentration_ng_ml": 1320.0, "mw_g_per_mol": 44000.0}, {"well": "A2", "label": "Chromogenic substrate stock (TMB), 5.0 mM", "volume_ul": 5000, "concentration_mM": 5.0}, {"well": "A3", "label": "PBS, 1×", "volume_ul": 15000}], "constraints": ["The instruction does not prescribe well layout, an acid stop, or a dilution factor.", "No working concentration and no dilution factor are in the instruction."], "instrument": {"wavelength_nm": 652, "epsilon_per_M_cm": 39000.0, "path_cm": 0.56, "path_basis": "Applied path is 0.28 cm times the well volume divided by 100 uL. At 200 uL that path is 0.56 cm.", "hrp_mw_g_per_mol": 44000.0, "initial_rate_elapsed_s": [3.0, 6.0, 9.0], "max_substrate_conversion": 0.15, "min_initial_rate_points": 3}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 2, "trace_sequence": 2, "timestamp": "2026-09-16T10:24:24+00:00", "logged_at": "2026-09-16T10:24:24.350000+00:00", "source_agent": "human", "event_id": "s8-stock", "event_type": "external_perturbation", "message": "The HRP stock is 30 nM, 2000 uL. That is the only enzyme concentration given for this run.", "rationale": "The HRP stock is 30 nM, 2000 uL. That is the only enzyme concentration given for this run.", "payload": {"well": "A1", "declared_concentration_nM": 30.0, "physical_concentration_nM": 30.0, "exact_concentration_nM": 30.0, "stock_status": "only_given", "declared_volume_ul": 2000, "physical_volume_ul": 2000, "disclosed_to_agents": true}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 3, "trace_sequence": 3, "timestamp": "2026-09-16T10:24:36+00:00", "logged_at": "2026-09-16T10:24:36.350000+00:00", "source_agent": "planner", "event_id": "plan-plan-s8-neat", "event_type": "plan", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "rationale": "The given stock is 30 nM. This first preparation uses 15.1515 uL of that stock, which is 100 ng/mL in a 200 uL well. I will measure whether that condition is suitable. Read each well 24 s after TMB is dispensed.", "canonical_operations": [{"action_id": "plan-s8-neat-B1-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:B1", "volume_ul": 15.151515151515152, "reagent": "hrp"}, {"action_id": "plan-s8-neat-B1-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:B1", "volume_ul": 152.84848484848484, "reagent": "buffer"}, {"action_id": "plan-s8-neat-B1-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:B1", "volume_ul": 32.0, "reagent": "tmb"}, {"action_id": "plan-s8-neat-B1-mix", "tool_name": "mix_well", "well": "B1"}, {"action_id": "plan-s8-neat-B2-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:B2", "volume_ul": 15.151515151515152, "reagent": "hrp"}, {"action_id": "plan-s8-neat-B2-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:B2", "volume_ul": 152.84848484848484, "reagent": "buffer"}, {"action_id": "plan-s8-neat-B2-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:B2", "volume_ul": 32.0, "reagent": "tmb"}, {"action_id": "plan-s8-neat-B2-mix", "tool_name": "mix_well", "well": "B2"}, {"action_id": "plan-s8-neat-B3-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:B3", "volume_ul": 15.151515151515152, "reagent": "hrp"}, {"action_id": "plan-s8-neat-B3-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:B3", "volume_ul": 152.84848484848484, "reagent": "buffer"}, {"action_id": "plan-s8-neat-B3-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:B3", "volume_ul": 32.0, "reagent": "tmb"}, {"action_id": "plan-s8-neat-B3-mix", "tool_name": "mix_well", "well": "B3"}, {"action_id": "plan-s8-neat-B4-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:B4", "volume_ul": 15.151515151515152, "reagent": "hrp"}, {"action_id": "plan-s8-neat-B4-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:B4", "volume_ul": 152.84848484848484, "reagent": "buffer"}, {"action_id": "plan-s8-neat-B4-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:B4", "volume_ul": 32.0, "reagent": "tmb"}, {"action_id": "plan-s8-neat-B4-mix", "tool_name": "mix_well", "well": "B4"}, {"action_id": "plan-s8-neat-B5-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:B5", "volume_ul": 15.151515151515152, "reagent": "hrp"}, {"action_id": "plan-s8-neat-B5-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:B5", "volume_ul": 152.84848484848484, "reagent": "buffer"}, {"action_id": "plan-s8-neat-B5-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:B5", "volume_ul": 32.0, "reagent": "tmb"}, {"action_id": "plan-s8-neat-B5-mix", "tool_name": "mix_well", "well": "B5"}]}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 4, "trace_sequence": 4, "timestamp": "2026-09-16T10:24:48+00:00", "logged_at": "2026-09-16T10:24:48.350000+00:00", "source_agent": null, "event_id": "appr-appr-plan-s8-neat", "event_type": "proceed_signal", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "approval_id": "appr-plan-s8-neat", "approved_action_ids": ["plan-s8-neat-B1-hrp", "plan-s8-neat-B1-buffer", "plan-s8-neat-B1-tmb", "plan-s8-neat-B1-mix", "plan-s8-neat-B2-hrp", "plan-s8-neat-B2-buffer", "plan-s8-neat-B2-tmb", "plan-s8-neat-B2-mix", "plan-s8-neat-B3-hrp", "plan-s8-neat-B3-buffer", "plan-s8-neat-B3-tmb", "plan-s8-neat-B3-mix", "plan-s8-neat-B4-hrp", "plan-s8-neat-B4-buffer", "plan-s8-neat-B4-tmb", "plan-s8-neat-B4-mix", "plan-s8-neat-B5-hrp", "plan-s8-neat-B5-buffer", "plan-s8-neat-B5-tmb", "plan-s8-neat-B5-mix"], "message": "Approved against the public deck and the stated instruction."}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 5, "trace_sequence": 5, "timestamp": "2026-09-16T10:24:57+00:00", "logged_at": "2026-09-16T10:24:57.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B1-hrp", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B1-hrp", "action_params": {"source": "A1", "dest": "B1", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-neat-B1-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 6, "trace_sequence": 6, "timestamp": "2026-09-16T10:25:04+00:00", "logged_at": "2026-09-16T10:25:04.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B1-buffer", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B1-buffer", "action_params": {"source": "A3", "dest": "B1", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-neat-B1-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 7, "trace_sequence": 7, "timestamp": "2026-09-16T10:25:13+00:00", "logged_at": "2026-09-16T10:25:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B1-tmb", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B1-tmb", "action_params": {"source": "A2", "dest": "B1", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-neat-B1-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 8, "trace_sequence": 8, "timestamp": "2026-09-16T10:25:15+00:00", "logged_at": "2026-09-16T10:25:15.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-neat-B1-mix", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B1-mix", "action_params": {"well": "B1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-neat-B1-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 9, "trace_sequence": 9, "timestamp": "2026-09-16T10:25:37+00:00", "logged_at": "2026-09-16T10:25:37.350000+00:00", "source_agent": null, "event_id": "obs-s8-neat-B1-t24", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-neat-B1-t24", "wells": {"plate:B1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock", "A652": 3.5, "elapsed_s": 24.0, "reaction_started_at": "2026-09-16T10:25:13+00:00", "acquired_at": "2026-09-16T10:25:37+00:00", "acid_stopped": false, "predicted_A652_uncapped": 4.0238545454545465, "saturated": true}}, "note": "This well is saturated, so the initial rate could not be estimated. It is not used for the fit."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 10, "trace_sequence": 10, "timestamp": "2026-09-16T10:25:45+00:00", "logged_at": "2026-09-16T10:25:45.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B2-hrp", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B2-hrp", "action_params": {"source": "A1", "dest": "B2", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-neat-B2-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 11, "trace_sequence": 11, "timestamp": "2026-09-16T10:25:53+00:00", "logged_at": "2026-09-16T10:25:53.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B2-buffer", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B2-buffer", "action_params": {"source": "A3", "dest": "B2", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-neat-B2-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 12, "trace_sequence": 12, "timestamp": "2026-09-16T10:26:01+00:00", "logged_at": "2026-09-16T10:26:01.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B2-tmb", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B2-tmb", "action_params": {"source": "A2", "dest": "B2", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-neat-B2-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 13, "trace_sequence": 13, "timestamp": "2026-09-16T10:26:02+00:00", "logged_at": "2026-09-16T10:26:02.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-neat-B2-mix", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B2-mix", "action_params": {"well": "B2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-neat-B2-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 14, "trace_sequence": 14, "timestamp": "2026-09-16T10:26:25+00:00", "logged_at": "2026-09-16T10:26:25.350000+00:00", "source_agent": null, "event_id": "obs-s8-neat-B2-t24", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-neat-B2-t24", "wells": {"plate:B2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock", "A652": 3.5, "elapsed_s": 24.0, "reaction_started_at": "2026-09-16T10:26:01+00:00", "acquired_at": "2026-09-16T10:26:25+00:00", "acid_stopped": false, "predicted_A652_uncapped": 4.0238545454545465, "saturated": true}}, "note": "This well is saturated, so the initial rate could not be estimated. It is not used for the fit."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 15, "trace_sequence": 15, "timestamp": "2026-09-16T10:26:39+00:00", "logged_at": "2026-09-16T10:26:39.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B3-hrp", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B3-hrp", "action_params": {"source": "A1", "dest": "B3", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-neat-B3-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 16, "trace_sequence": 16, "timestamp": "2026-09-16T10:26:44+00:00", "logged_at": "2026-09-16T10:26:44.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B3-buffer", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B3-buffer", "action_params": {"source": "A3", "dest": "B3", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-neat-B3-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 17, "trace_sequence": 17, "timestamp": "2026-09-16T10:26:51+00:00", "logged_at": "2026-09-16T10:26:51.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B3-tmb", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B3-tmb", "action_params": {"source": "A2", "dest": "B3", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-neat-B3-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 18, "trace_sequence": 18, "timestamp": "2026-09-16T10:26:52+00:00", "logged_at": "2026-09-16T10:26:52.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-neat-B3-mix", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B3-mix", "action_params": {"well": "B3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-neat-B3-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 19, "trace_sequence": 19, "timestamp": "2026-09-16T10:27:15+00:00", "logged_at": "2026-09-16T10:27:15.350000+00:00", "source_agent": null, "event_id": "obs-s8-neat-B3-t24", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-neat-B3-t24", "wells": {"plate:B3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock", "A652": 3.5, "elapsed_s": 24.0, "reaction_started_at": "2026-09-16T10:26:51+00:00", "acquired_at": "2026-09-16T10:27:15+00:00", "acid_stopped": false, "predicted_A652_uncapped": 4.0238545454545465, "saturated": true}}, "note": "This well is saturated, so the initial rate could not be estimated. It is not used for the fit."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 20, "trace_sequence": 20, "timestamp": "2026-09-16T10:27:27+00:00", "logged_at": "2026-09-16T10:27:27.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B4-hrp", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B4-hrp", "action_params": {"source": "A1", "dest": "B4", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-neat-B4-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 21, "trace_sequence": 21, "timestamp": "2026-09-16T10:27:34+00:00", "logged_at": "2026-09-16T10:27:34.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B4-buffer", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B4-buffer", "action_params": {"source": "A3", "dest": "B4", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-neat-B4-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 22, "trace_sequence": 22, "timestamp": "2026-09-16T10:27:41+00:00", "logged_at": "2026-09-16T10:27:41.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B4-tmb", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B4-tmb", "action_params": {"source": "A2", "dest": "B4", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-neat-B4-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 23, "trace_sequence": 23, "timestamp": "2026-09-16T10:27:42+00:00", "logged_at": "2026-09-16T10:27:42.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-neat-B4-mix", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B4-mix", "action_params": {"well": "B4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-neat-B4-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 24, "trace_sequence": 24, "timestamp": "2026-09-16T10:28:05+00:00", "logged_at": "2026-09-16T10:28:05.350000+00:00", "source_agent": null, "event_id": "obs-s8-neat-B4-t24", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-neat-B4-t24", "wells": {"plate:B4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock", "A652": 3.5, "elapsed_s": 24.0, "reaction_started_at": "2026-09-16T10:27:41+00:00", "acquired_at": "2026-09-16T10:28:05+00:00", "acid_stopped": false, "predicted_A652_uncapped": 4.0238545454545465, "saturated": true}}, "note": "This well is saturated, so the initial rate could not be estimated. It is not used for the fit."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 25, "trace_sequence": 25, "timestamp": "2026-09-16T10:28:16+00:00", "logged_at": "2026-09-16T10:28:16.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B5-hrp", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B5-hrp", "action_params": {"source": "A1", "dest": "B5", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-neat-B5-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 26, "trace_sequence": 26, "timestamp": "2026-09-16T10:28:21+00:00", "logged_at": "2026-09-16T10:28:21.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B5-buffer", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B5-buffer", "action_params": {"source": "A3", "dest": "B5", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-neat-B5-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 27, "trace_sequence": 27, "timestamp": "2026-09-16T10:28:30+00:00", "logged_at": "2026-09-16T10:28:30.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-neat-B5-tmb", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B5-tmb", "action_params": {"source": "A2", "dest": "B5", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-neat-B5-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 28, "trace_sequence": 28, "timestamp": "2026-09-16T10:28:32+00:00", "logged_at": "2026-09-16T10:28:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-neat-B5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-neat-B5-mix", "plan_id": "plan-s8-neat", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-neat-B5-mix", "action_params": {"well": "B5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-neat-B5-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 29, "trace_sequence": 29, "timestamp": "2026-09-16T10:28:54+00:00", "logged_at": "2026-09-16T10:28:54.350000+00:00", "source_agent": null, "event_id": "obs-s8-neat-B5-t24", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-neat-B5-t24", "wells": {"plate:B5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 2.272727272727273, "enzyme_concentration_ng_ml": 100.0, "enzyme_concentration_M": 2.272727272727273e-09, "rate_A652_per_min": 10.059636363636367, "rate_uM_per_s": 7.676767676767678, "series": "saturated_attempt", "initial_rate_eligible": false, "used_for_fit": false, "concentration_basis": "exact_stock", "A652": 3.5, "elapsed_s": 24.0, "reaction_started_at": "2026-09-16T10:28:30+00:00", "acquired_at": "2026-09-16T10:28:54+00:00", "acid_stopped": false, "predicted_A652_uncapped": 4.0238545454545465, "saturated": true}}, "note": "This well is saturated, so the initial rate could not be estimated. It is not used for the fit."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 30, "trace_sequence": 30, "timestamp": "2026-09-16T10:28:54+00:00", "logged_at": "2026-09-16T10:28:54.350000+00:00", "source_agent": "knowledge", "event_id": "know-protocol-hrp-tmb-initial-rate-curve", "event_type": "observation", "observation_type": "knowledge_response", "payload": {"source_id": "protocol-hrp-tmb-initial-rate-curve", "text": "Assay guidance for the HRP-TMB initial-rate assay. At 200 uM TMB, path 0.56 cm, reads at 3, 6, and 9 s, and no acid stop. The dilution is chosen from this run's measurement.", "protocol_id": "protocol-hrp-tmb-initial-rate-curve", "category": "protocol", "conditions": {"substrate_uM": 200.0, "path_cm": 0.56, "elapsed_s": [3.0, 6.0, 9.0], "acid_stop": false}}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 31, "trace_sequence": 31, "timestamp": "2026-09-16T10:29:06+00:00", "logged_at": "2026-09-16T10:29:06.350000+00:00", "source_agent": "planner", "event_id": "msg-0031", "event_type": "recovery_decision", "rationale": "The first wells hit the ceiling. They were prepared at 100 ng/mL from the given 30 nM stock, and the reaction is too fast. That concentration is too high. Hypothesis: I will prepare new wells from a dilution of that stock and search for a dilution factor that brings the rate back on scale.", "message": "The first wells hit the ceiling. They were prepared at 100 ng/mL from the given 30 nM stock, and the reaction is too fast. That concentration is too high. Hypothesis: I will prepare new wells from a dilution of that stock and search for a dilution factor that brings the rate back on scale."}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 32, "trace_sequence": 32, "timestamp": "2026-09-16T10:29:18+00:00", "logged_at": "2026-09-16T10:29:18.350000+00:00", "source_agent": "planner", "event_id": "plan-plan-s8-diluted", "event_type": "plan", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "rationale": "The first wells were 100 ng/mL from the given 30 nM stock and saturated. I will prepare new wells, not by transferring that saturated reaction. I will search a series of 0.5, 1, 2, 5, 10, 20, 40, and 80 ng/mL, with volumes computed from that 30 nM stock, and see which points stay proportional. The aliquot in H12 is a 14-fold dilution, 20 uL of the 30 nM stock plus 260 uL PBS, so the 0.5 ng/mL transfer is at least 1 uL. The substrate series is dispensed at 5 ng/mL from the same stock. Read each well at 3, 6, and 9 s after TMB is dispensed. A blank matches each substrate concentration.", "canonical_operations": [{"action_id": "plan-s8-diluted-H12-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:H12", "volume_ul": 20.0, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-H12-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:H12", "volume_ul": 260.0, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-H12-mix", "tool_name": "mix_well", "well": "H12"}, {"action_id": "plan-s8-diluted-A1-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:A1", "volume_ul": 1.0606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A1-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A1", "volume_ul": 190.93939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A1-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A1", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A1-mix", "tool_name": "mix_well", "well": "A1"}, {"action_id": "plan-s8-diluted-A2-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:A2", "volume_ul": 2.121212121212121, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A2-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A2", "volume_ul": 189.87878787878788, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A2-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A2", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A2-mix", "tool_name": "mix_well", "well": "A2"}, {"action_id": "plan-s8-diluted-A3-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:A3", "volume_ul": 4.242424242424242, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A3-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A3", "volume_ul": 187.75757575757575, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A3-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A3", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A3-mix", "tool_name": "mix_well", "well": "A3"}, {"action_id": "plan-s8-diluted-A4-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:A4", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A4-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A4", "volume_ul": 181.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A4-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A4", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A4-mix", "tool_name": "mix_well", "well": "A4"}, {"action_id": "plan-s8-diluted-A5-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:A5", "volume_ul": 1.5151515151515151, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A5-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A5", "volume_ul": 190.4848484848485, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A5-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A5", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A5-mix", "tool_name": "mix_well", "well": "A5"}, {"action_id": "plan-s8-diluted-A6-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:A6", "volume_ul": 3.0303030303030303, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A6-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A6", "volume_ul": 188.96969696969697, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A6-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A6", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A6-mix", "tool_name": "mix_well", "well": "A6"}, {"action_id": "plan-s8-diluted-A7-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:A7", "volume_ul": 6.0606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A7-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A7", "volume_ul": 185.93939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A7-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A7", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A7-mix", "tool_name": "mix_well", "well": "A7"}, {"action_id": "plan-s8-diluted-A8-hrp", "tool_name": "transfer", "source": "reservoir:A1", "dest": "plate:A8", "volume_ul": 12.121212121212121, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-A8-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:A8", "volume_ul": 179.87878787878788, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-A8-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:A8", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-A8-mix", "tool_name": "mix_well", "well": "A8"}, {"action_id": "plan-s8-diluted-F1-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:F1", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-F1-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:F1", "volume_ul": 188.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-F1-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:F1", "volume_ul": 1.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-F1-mix", "tool_name": "mix_well", "well": "F1"}, {"action_id": "plan-s8-diluted-G1-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:G1", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-G1-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:G1", "volume_ul": 188.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-G1-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:G1", "volume_ul": 1.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-G1-mix", "tool_name": "mix_well", "well": "G1"}, {"action_id": "plan-s8-diluted-F2-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:F2", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-F2-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:F2", "volume_ul": 187.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-F2-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:F2", "volume_ul": 2.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-F2-mix", "tool_name": "mix_well", "well": "F2"}, {"action_id": "plan-s8-diluted-G2-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:G2", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-G2-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:G2", "volume_ul": 187.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-G2-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:G2", "volume_ul": 2.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-G2-mix", "tool_name": "mix_well", "well": "G2"}, {"action_id": "plan-s8-diluted-F3-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:F3", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-F3-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:F3", "volume_ul": 185.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-F3-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:F3", "volume_ul": 4.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-F3-mix", "tool_name": "mix_well", "well": "F3"}, {"action_id": "plan-s8-diluted-G3-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:G3", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-G3-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:G3", "volume_ul": 185.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-G3-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:G3", "volume_ul": 4.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-G3-mix", "tool_name": "mix_well", "well": "G3"}, {"action_id": "plan-s8-diluted-F4-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:F4", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-F4-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:F4", "volume_ul": 181.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-F4-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:F4", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-F4-mix", "tool_name": "mix_well", "well": "F4"}, {"action_id": "plan-s8-diluted-G4-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:G4", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-G4-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:G4", "volume_ul": 181.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-G4-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:G4", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-G4-mix", "tool_name": "mix_well", "well": "G4"}, {"action_id": "plan-s8-diluted-F5-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:F5", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-F5-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:F5", "volume_ul": 173.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-F5-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:F5", "volume_ul": 16.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-F5-mix", "tool_name": "mix_well", "well": "F5"}, {"action_id": "plan-s8-diluted-G5-hrp", "tool_name": "transfer", "source": "plate:H12", "dest": "plate:G5", "volume_ul": 10.606060606060606, "reagent": "hrp"}, {"action_id": "plan-s8-diluted-G5-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:G5", "volume_ul": 173.3939393939394, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-G5-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:G5", "volume_ul": 16.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-G5-mix", "tool_name": "mix_well", "well": "G5"}, {"action_id": "plan-s8-diluted-E1-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:E1", "volume_ul": 199.0, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-E1-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:E1", "volume_ul": 1.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-E1-mix", "tool_name": "mix_well", "well": "E1"}, {"action_id": "plan-s8-diluted-E2-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:E2", "volume_ul": 198.0, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-E2-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:E2", "volume_ul": 2.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-E2-mix", "tool_name": "mix_well", "well": "E2"}, {"action_id": "plan-s8-diluted-E3-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:E3", "volume_ul": 196.0, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-E3-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:E3", "volume_ul": 4.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-E3-mix", "tool_name": "mix_well", "well": "E3"}, {"action_id": "plan-s8-diluted-E4-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:E4", "volume_ul": 192.0, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-E4-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:E4", "volume_ul": 8.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-E4-mix", "tool_name": "mix_well", "well": "E4"}, {"action_id": "plan-s8-diluted-E5-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:E5", "volume_ul": 184.0, "reagent": "buffer"}, {"action_id": "plan-s8-diluted-E5-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:E5", "volume_ul": 16.0, "reagent": "tmb"}, {"action_id": "plan-s8-diluted-E5-mix", "tool_name": "mix_well", "well": "E5"}]}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 33, "trace_sequence": 33, "timestamp": "2026-09-16T10:29:30+00:00", "logged_at": "2026-09-16T10:29:30.350000+00:00", "source_agent": null, "event_id": "appr-appr-plan-s8-diluted", "event_type": "proceed_signal", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "approval_id": "appr-plan-s8-diluted", "approved_action_ids": ["plan-s8-diluted-H12-hrp", "plan-s8-diluted-H12-buffer", "plan-s8-diluted-H12-mix", "plan-s8-diluted-A1-hrp", "plan-s8-diluted-A1-buffer", "plan-s8-diluted-A1-tmb", "plan-s8-diluted-A1-mix", "plan-s8-diluted-A2-hrp", "plan-s8-diluted-A2-buffer", "plan-s8-diluted-A2-tmb", "plan-s8-diluted-A2-mix", "plan-s8-diluted-A3-hrp", "plan-s8-diluted-A3-buffer", "plan-s8-diluted-A3-tmb", "plan-s8-diluted-A3-mix", "plan-s8-diluted-A4-hrp", "plan-s8-diluted-A4-buffer", "plan-s8-diluted-A4-tmb", "plan-s8-diluted-A4-mix", "plan-s8-diluted-A5-hrp", "plan-s8-diluted-A5-buffer", "plan-s8-diluted-A5-tmb", "plan-s8-diluted-A5-mix", "plan-s8-diluted-A6-hrp", "plan-s8-diluted-A6-buffer", "plan-s8-diluted-A6-tmb", "plan-s8-diluted-A6-mix", "plan-s8-diluted-A7-hrp", "plan-s8-diluted-A7-buffer", "plan-s8-diluted-A7-tmb", "plan-s8-diluted-A7-mix", "plan-s8-diluted-A8-hrp", "plan-s8-diluted-A8-buffer", "plan-s8-diluted-A8-tmb", "plan-s8-diluted-A8-mix", "plan-s8-diluted-F1-hrp", "plan-s8-diluted-F1-buffer", "plan-s8-diluted-F1-tmb", "plan-s8-diluted-F1-mix", "plan-s8-diluted-G1-hrp", "plan-s8-diluted-G1-buffer", "plan-s8-diluted-G1-tmb", "plan-s8-diluted-G1-mix", "plan-s8-diluted-F2-hrp", "plan-s8-diluted-F2-buffer", "plan-s8-diluted-F2-tmb", "plan-s8-diluted-F2-mix", "plan-s8-diluted-G2-hrp", "plan-s8-diluted-G2-buffer", "plan-s8-diluted-G2-tmb", "plan-s8-diluted-G2-mix", "plan-s8-diluted-F3-hrp", "plan-s8-diluted-F3-buffer", "plan-s8-diluted-F3-tmb", "plan-s8-diluted-F3-mix", "plan-s8-diluted-G3-hrp", "plan-s8-diluted-G3-buffer", "plan-s8-diluted-G3-tmb", "plan-s8-diluted-G3-mix", "plan-s8-diluted-F4-hrp", "plan-s8-diluted-F4-buffer", "plan-s8-diluted-F4-tmb", "plan-s8-diluted-F4-mix", "plan-s8-diluted-G4-hrp", "plan-s8-diluted-G4-buffer", "plan-s8-diluted-G4-tmb", "plan-s8-diluted-G4-mix", "plan-s8-diluted-F5-hrp", "plan-s8-diluted-F5-buffer", "plan-s8-diluted-F5-tmb", "plan-s8-diluted-F5-mix", "plan-s8-diluted-G5-hrp", "plan-s8-diluted-G5-buffer", "plan-s8-diluted-G5-tmb", "plan-s8-diluted-G5-mix", "plan-s8-diluted-E1-buffer", "plan-s8-diluted-E1-tmb", "plan-s8-diluted-E1-mix", "plan-s8-diluted-E2-buffer", "plan-s8-diluted-E2-tmb", "plan-s8-diluted-E2-mix", "plan-s8-diluted-E3-buffer", "plan-s8-diluted-E3-tmb", "plan-s8-diluted-E3-mix", "plan-s8-diluted-E4-buffer", "plan-s8-diluted-E4-tmb", "plan-s8-diluted-E4-mix", "plan-s8-diluted-E5-buffer", "plan-s8-diluted-E5-tmb", "plan-s8-diluted-E5-mix"], "message": "Approved against the public deck and the stated instruction."}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 34, "trace_sequence": 34, "timestamp": "2026-09-16T10:29:40+00:00", "logged_at": "2026-09-16T10:29:40.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-H12-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-H12-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-H12-hrp", "action_params": {"source": "A1", "dest": "H12", "volume_ul": 20.0, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:H12", "volume_ul": 20.0, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-H12-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 35, "trace_sequence": 35, "timestamp": "2026-09-16T10:29:45+00:00", "logged_at": "2026-09-16T10:29:45.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-H12-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-H12-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-H12-buffer", "action_params": {"source": "A3", "dest": "H12", "volume_ul": 260.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:H12", "volume_ul": 260.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-H12-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 36, "trace_sequence": 36, "timestamp": "2026-09-16T10:29:46+00:00", "logged_at": "2026-09-16T10:29:46.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-H12-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-H12-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-H12-mix", "action_params": {"well": "H12"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:H12"}}, "result_observation_ids": ["obs-plan-s8-diluted-H12-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 37, "trace_sequence": 37, "timestamp": "2026-09-16T10:29:58+00:00", "logged_at": "2026-09-16T10:29:58.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A1-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A1-hrp", "action_params": {"source": "H12", "dest": "A1", "volume_ul": 1.0606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "volume_ul": 1.0606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A1-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 38, "trace_sequence": 38, "timestamp": "2026-09-16T10:30:04+00:00", "logged_at": "2026-09-16T10:30:04.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A1-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A1-buffer", "action_params": {"source": "A3", "dest": "A1", "volume_ul": 190.93939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "volume_ul": 190.93939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A1-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 39, "trace_sequence": 39, "timestamp": "2026-09-16T10:30:12+00:00", "logged_at": "2026-09-16T10:30:12.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A1-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A1-tmb", "action_params": {"source": "A2", "dest": "A1", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A1-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 40, "trace_sequence": 40, "timestamp": "2026-09-16T10:30:14+00:00", "logged_at": "2026-09-16T10:30:14.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A1-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A1-mix", "action_params": {"well": "A1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "enzyme_volume_ul": 1.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "rate_A652_per_min": 0.03772363636363637, "rate_uM_per_s": 0.028787878787878782, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.07575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A1-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 41, "trace_sequence": 41, "timestamp": "2026-09-16T10:30:15+00:00", "logged_at": "2026-09-16T10:30:15.350000+00:00", "source_agent": null, "event_id": "obs-s8-A1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A1-t3", "wells": {"plate:A1": {"enzyme_volume_ul": 1.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "rate_A652_per_min": 0.03772363636363637, "rate_uM_per_s": 0.028787878787878782, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.07575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.0018861818181818183, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:30:12+00:00", "acquired_at": "2026-09-16T10:30:15+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00043181818181818176}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 42, "trace_sequence": 42, "timestamp": "2026-09-16T10:30:18+00:00", "logged_at": "2026-09-16T10:30:18.350000+00:00", "source_agent": null, "event_id": "obs-s8-A1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A1-t6", "wells": {"plate:A1": {"enzyme_volume_ul": 1.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "rate_A652_per_min": 0.03772363636363637, "rate_uM_per_s": 0.028787878787878782, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.07575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.0037723636363636366, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:30:12+00:00", "acquired_at": "2026-09-16T10:30:18+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0008636363636363635}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 43, "trace_sequence": 43, "timestamp": "2026-09-16T10:30:21+00:00", "logged_at": "2026-09-16T10:30:21.350000+00:00", "source_agent": null, "event_id": "obs-s8-A1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A1-t9", "wells": {"plate:A1": {"enzyme_volume_ul": 1.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "rate_A652_per_min": 0.03772363636363637, "rate_uM_per_s": 0.028787878787878782, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.07575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.005658545454545455, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:30:12+00:00", "acquired_at": "2026-09-16T10:30:21+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0012954545454545452}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 44, "trace_sequence": 44, "timestamp": "2026-09-16T10:30:31+00:00", "logged_at": "2026-09-16T10:30:31.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A2-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A2-hrp", "action_params": {"source": "H12", "dest": "A2", "volume_ul": 2.121212121212121, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "volume_ul": 2.121212121212121, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A2-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 45, "trace_sequence": 45, "timestamp": "2026-09-16T10:30:40+00:00", "logged_at": "2026-09-16T10:30:40.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A2-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A2-buffer", "action_params": {"source": "A3", "dest": "A2", "volume_ul": 189.87878787878788, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "volume_ul": 189.87878787878788, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A2-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 46, "trace_sequence": 46, "timestamp": "2026-09-16T10:30:48+00:00", "logged_at": "2026-09-16T10:30:48.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A2-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A2-tmb", "action_params": {"source": "A2", "dest": "A2", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A2-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 47, "trace_sequence": 47, "timestamp": "2026-09-16T10:30:50+00:00", "logged_at": "2026-09-16T10:30:50.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A2-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A2-mix", "action_params": {"well": "A2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "enzyme_volume_ul": 2.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 189.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07544727272727274, "rate_uM_per_s": 0.057575757575757565, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.15151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A2-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 48, "trace_sequence": 48, "timestamp": "2026-09-16T10:30:51+00:00", "logged_at": "2026-09-16T10:30:51.350000+00:00", "source_agent": null, "event_id": "obs-s8-A2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A2-t3", "wells": {"plate:A2": {"enzyme_volume_ul": 2.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 189.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07544727272727274, "rate_uM_per_s": 0.057575757575757565, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.15151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.0037723636363636366, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:30:48+00:00", "acquired_at": "2026-09-16T10:30:51+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0008636363636363635}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 49, "trace_sequence": 49, "timestamp": "2026-09-16T10:30:54+00:00", "logged_at": "2026-09-16T10:30:54.350000+00:00", "source_agent": null, "event_id": "obs-s8-A2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A2-t6", "wells": {"plate:A2": {"enzyme_volume_ul": 2.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 189.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07544727272727274, "rate_uM_per_s": 0.057575757575757565, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.15151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.007544727272727273, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:30:48+00:00", "acquired_at": "2026-09-16T10:30:54+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.001727272727272727}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 50, "trace_sequence": 50, "timestamp": "2026-09-16T10:30:57+00:00", "logged_at": "2026-09-16T10:30:57.350000+00:00", "source_agent": null, "event_id": "obs-s8-A2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A2-t9", "wells": {"plate:A2": {"enzyme_volume_ul": 2.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 189.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07544727272727274, "rate_uM_per_s": 0.057575757575757565, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.15151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.01131709090909091, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:30:48+00:00", "acquired_at": "2026-09-16T10:30:57+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0025909090909090903}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 51, "trace_sequence": 51, "timestamp": "2026-09-16T10:31:12+00:00", "logged_at": "2026-09-16T10:31:12.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A3-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A3-hrp", "action_params": {"source": "H12", "dest": "A3", "volume_ul": 4.242424242424242, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "volume_ul": 4.242424242424242, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A3-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 52, "trace_sequence": 52, "timestamp": "2026-09-16T10:31:18+00:00", "logged_at": "2026-09-16T10:31:18.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A3-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A3-buffer", "action_params": {"source": "A3", "dest": "A3", "volume_ul": 187.75757575757575, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "volume_ul": 187.75757575757575, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A3-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 53, "trace_sequence": 53, "timestamp": "2026-09-16T10:31:25+00:00", "logged_at": "2026-09-16T10:31:25.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A3-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A3-tmb", "action_params": {"source": "A2", "dest": "A3", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A3-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 54, "trace_sequence": 54, "timestamp": "2026-09-16T10:31:27+00:00", "logged_at": "2026-09-16T10:31:27.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A3-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A3-mix", "action_params": {"well": "A3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "enzyme_volume_ul": 4.242424242424242, "tmb_volume_ul": 8.0, "buffer_volume_ul": 187.75757575757575, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15089454545454548, "rate_uM_per_s": 0.11515151515151513, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.30303030303030304, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A3-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 55, "trace_sequence": 55, "timestamp": "2026-09-16T10:31:28+00:00", "logged_at": "2026-09-16T10:31:28.350000+00:00", "source_agent": null, "event_id": "obs-s8-A3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A3-t3", "wells": {"plate:A3": {"enzyme_volume_ul": 4.242424242424242, "tmb_volume_ul": 8.0, "buffer_volume_ul": 187.75757575757575, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15089454545454548, "rate_uM_per_s": 0.11515151515151513, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.30303030303030304, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.007544727272727273, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:31:25+00:00", "acquired_at": "2026-09-16T10:31:28+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.001727272727272727}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 56, "trace_sequence": 56, "timestamp": "2026-09-16T10:31:31+00:00", "logged_at": "2026-09-16T10:31:31.350000+00:00", "source_agent": null, "event_id": "obs-s8-A3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A3-t6", "wells": {"plate:A3": {"enzyme_volume_ul": 4.242424242424242, "tmb_volume_ul": 8.0, "buffer_volume_ul": 187.75757575757575, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15089454545454548, "rate_uM_per_s": 0.11515151515151513, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.30303030303030304, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.015089454545454547, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:31:25+00:00", "acquired_at": "2026-09-16T10:31:31+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.003454545454545454}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 57, "trace_sequence": 57, "timestamp": "2026-09-16T10:31:34+00:00", "logged_at": "2026-09-16T10:31:34.350000+00:00", "source_agent": null, "event_id": "obs-s8-A3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A3-t9", "wells": {"plate:A3": {"enzyme_volume_ul": 4.242424242424242, "tmb_volume_ul": 8.0, "buffer_volume_ul": 187.75757575757575, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15089454545454548, "rate_uM_per_s": 0.11515151515151513, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.30303030303030304, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.02263418181818182, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:31:25+00:00", "acquired_at": "2026-09-16T10:31:34+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005181818181818181}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 58, "trace_sequence": 58, "timestamp": "2026-09-16T10:31:49+00:00", "logged_at": "2026-09-16T10:31:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A4-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A4-hrp", "action_params": {"source": "H12", "dest": "A4", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A4-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 59, "trace_sequence": 59, "timestamp": "2026-09-16T10:31:53+00:00", "logged_at": "2026-09-16T10:31:53.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A4-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A4-buffer", "action_params": {"source": "A3", "dest": "A4", "volume_ul": 181.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "volume_ul": 181.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A4-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 60, "trace_sequence": 60, "timestamp": "2026-09-16T10:32:02+00:00", "logged_at": "2026-09-16T10:32:02.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A4-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A4-tmb", "action_params": {"source": "A2", "dest": "A4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A4-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 61, "trace_sequence": 61, "timestamp": "2026-09-16T10:32:04+00:00", "logged_at": "2026-09-16T10:32:04.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A4-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A4-mix", "action_params": {"well": "A4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3772363636363637, "rate_uM_per_s": 0.2878787878787879, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A4-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 62, "trace_sequence": 62, "timestamp": "2026-09-16T10:32:05+00:00", "logged_at": "2026-09-16T10:32:05.350000+00:00", "source_agent": null, "event_id": "obs-s8-A4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A4-t3", "wells": {"plate:A4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3772363636363637, "rate_uM_per_s": 0.2878787878787879, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.018861818181818188, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:32:02+00:00", "acquired_at": "2026-09-16T10:32:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.004318181818181819}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 63, "trace_sequence": 63, "timestamp": "2026-09-16T10:32:08+00:00", "logged_at": "2026-09-16T10:32:08.350000+00:00", "source_agent": null, "event_id": "obs-s8-A4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A4-t6", "wells": {"plate:A4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3772363636363637, "rate_uM_per_s": 0.2878787878787879, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.037723636363636376, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:32:02+00:00", "acquired_at": "2026-09-16T10:32:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008636363636363638}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 64, "trace_sequence": 64, "timestamp": "2026-09-16T10:32:11+00:00", "logged_at": "2026-09-16T10:32:11.350000+00:00", "source_agent": null, "event_id": "obs-s8-A4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A4-t9", "wells": {"plate:A4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3772363636363637, "rate_uM_per_s": 0.2878787878787879, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.05658545454545456, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:32:02+00:00", "acquired_at": "2026-09-16T10:32:11+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.012954545454545456}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 65, "trace_sequence": 65, "timestamp": "2026-09-16T10:32:20+00:00", "logged_at": "2026-09-16T10:32:20.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A5-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A5-hrp", "action_params": {"source": "A1", "dest": "A5", "volume_ul": 1.5151515151515151, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "volume_ul": 1.5151515151515151, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A5-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 66, "trace_sequence": 66, "timestamp": "2026-09-16T10:32:29+00:00", "logged_at": "2026-09-16T10:32:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A5-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A5-buffer", "action_params": {"source": "A3", "dest": "A5", "volume_ul": 190.4848484848485, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "volume_ul": 190.4848484848485, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A5-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 67, "trace_sequence": 67, "timestamp": "2026-09-16T10:32:34+00:00", "logged_at": "2026-09-16T10:32:34.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A5-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A5-tmb", "action_params": {"source": "A2", "dest": "A5", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A5-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 68, "trace_sequence": 68, "timestamp": "2026-09-16T10:32:36+00:00", "logged_at": "2026-09-16T10:32:36.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A5-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A5-mix", "action_params": {"well": "A5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.712557575757576, "rate_uM_per_s": 0.5437710437710438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A5-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 69, "trace_sequence": 69, "timestamp": "2026-09-16T10:32:37+00:00", "logged_at": "2026-09-16T10:32:37.350000+00:00", "source_agent": null, "event_id": "obs-s8-A5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A5-t3", "wells": {"plate:A5": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.712557575757576, "rate_uM_per_s": 0.5437710437710438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.03562787878787879, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:32:34+00:00", "acquired_at": "2026-09-16T10:32:37+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008156565656565657}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 70, "trace_sequence": 70, "timestamp": "2026-09-16T10:32:40+00:00", "logged_at": "2026-09-16T10:32:40.350000+00:00", "source_agent": null, "event_id": "obs-s8-A5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A5-t6", "wells": {"plate:A5": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.712557575757576, "rate_uM_per_s": 0.5437710437710438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.07125575757575758, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:32:34+00:00", "acquired_at": "2026-09-16T10:32:40+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.016313131313131314}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 71, "trace_sequence": 71, "timestamp": "2026-09-16T10:32:43+00:00", "logged_at": "2026-09-16T10:32:43.350000+00:00", "source_agent": null, "event_id": "obs-s8-A5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A5-t9", "wells": {"plate:A5": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.712557575757576, "rate_uM_per_s": 0.5437710437710438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.10688363636363639, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:32:34+00:00", "acquired_at": "2026-09-16T10:32:43+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.02446969696969697}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 72, "trace_sequence": 72, "timestamp": "2026-09-16T10:32:59+00:00", "logged_at": "2026-09-16T10:32:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A6-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A6-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A6-hrp", "action_params": {"source": "A1", "dest": "A6", "volume_ul": 3.0303030303030303, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "volume_ul": 3.0303030303030303, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A6-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 73, "trace_sequence": 73, "timestamp": "2026-09-16T10:33:05+00:00", "logged_at": "2026-09-16T10:33:05.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A6-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A6-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A6-buffer", "action_params": {"source": "A3", "dest": "A6", "volume_ul": 188.96969696969697, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "volume_ul": 188.96969696969697, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A6-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 74, "trace_sequence": 74, "timestamp": "2026-09-16T10:33:13+00:00", "logged_at": "2026-09-16T10:33:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A6-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A6-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A6-tmb", "action_params": {"source": "A2", "dest": "A6", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A6-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 75, "trace_sequence": 75, "timestamp": "2026-09-16T10:33:14+00:00", "logged_at": "2026-09-16T10:33:14.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A6-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A6-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A6-mix", "action_params": {"well": "A6"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.2826036363636366, "rate_uM_per_s": 0.9787878787878789, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A6-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 76, "trace_sequence": 76, "timestamp": "2026-09-16T10:33:16+00:00", "logged_at": "2026-09-16T10:33:16.350000+00:00", "source_agent": null, "event_id": "obs-s8-A6-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A6-t3", "wells": {"plate:A6": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.2826036363636366, "rate_uM_per_s": 0.9787878787878789, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.06413018181818184, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:33:13+00:00", "acquired_at": "2026-09-16T10:33:16+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.014681818181818183}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 77, "trace_sequence": 77, "timestamp": "2026-09-16T10:33:19+00:00", "logged_at": "2026-09-16T10:33:19.350000+00:00", "source_agent": null, "event_id": "obs-s8-A6-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A6-t6", "wells": {"plate:A6": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.2826036363636366, "rate_uM_per_s": 0.9787878787878789, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.12826036363636367, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:33:13+00:00", "acquired_at": "2026-09-16T10:33:19+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.029363636363636366}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 78, "trace_sequence": 78, "timestamp": "2026-09-16T10:33:22+00:00", "logged_at": "2026-09-16T10:33:22.350000+00:00", "source_agent": null, "event_id": "obs-s8-A6-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A6-t9", "wells": {"plate:A6": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.2826036363636366, "rate_uM_per_s": 0.9787878787878789, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.1923905454545455, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:33:13+00:00", "acquired_at": "2026-09-16T10:33:22+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.044045454545454554}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 79, "trace_sequence": 79, "timestamp": "2026-09-16T10:33:32+00:00", "logged_at": "2026-09-16T10:33:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A7-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A7-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A7-hrp", "action_params": {"source": "A1", "dest": "A7", "volume_ul": 6.0606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "volume_ul": 6.0606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A7-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 80, "trace_sequence": 80, "timestamp": "2026-09-16T10:33:37+00:00", "logged_at": "2026-09-16T10:33:37.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A7-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A7-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A7-buffer", "action_params": {"source": "A3", "dest": "A7", "volume_ul": 185.93939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "volume_ul": 185.93939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A7-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 81, "trace_sequence": 81, "timestamp": "2026-09-16T10:33:45+00:00", "logged_at": "2026-09-16T10:33:45.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A7-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A7-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A7-tmb", "action_params": {"source": "A2", "dest": "A7", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A7-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 82, "trace_sequence": 82, "timestamp": "2026-09-16T10:33:47+00:00", "logged_at": "2026-09-16T10:33:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A7-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A7-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A7-mix", "action_params": {"well": "A7"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "enzyme_volume_ul": 6.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 185.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.1376727272727276, "rate_uM_per_s": 1.6313131313131313, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 6.0606060606060606, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A7-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 83, "trace_sequence": 83, "timestamp": "2026-09-16T10:33:48+00:00", "logged_at": "2026-09-16T10:33:48.350000+00:00", "source_agent": null, "event_id": "obs-s8-A7-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A7-t3", "wells": {"plate:A7": {"enzyme_volume_ul": 6.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 185.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.1376727272727276, "rate_uM_per_s": 1.6313131313131313, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 6.0606060606060606, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.10688363636363638, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:33:45+00:00", "acquired_at": "2026-09-16T10:33:48+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.024469696969696968}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 84, "trace_sequence": 84, "timestamp": "2026-09-16T10:33:51+00:00", "logged_at": "2026-09-16T10:33:51.350000+00:00", "source_agent": null, "event_id": "obs-s8-A7-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A7-t6", "wells": {"plate:A7": {"enzyme_volume_ul": 6.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 185.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.1376727272727276, "rate_uM_per_s": 1.6313131313131313, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 6.0606060606060606, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.21376727272727275, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:33:45+00:00", "acquired_at": "2026-09-16T10:33:51+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.048939393939393935}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 85, "trace_sequence": 85, "timestamp": "2026-09-16T10:33:54+00:00", "logged_at": "2026-09-16T10:33:54.350000+00:00", "source_agent": null, "event_id": "obs-s8-A7-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A7-t9", "wells": {"plate:A7": {"enzyme_volume_ul": 6.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 185.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.1376727272727276, "rate_uM_per_s": 1.6313131313131313, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 6.0606060606060606, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.32065090909090915, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:33:45+00:00", "acquired_at": "2026-09-16T10:33:54+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.07340909090909091}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 86, "trace_sequence": 86, "timestamp": "2026-09-16T10:34:04+00:00", "logged_at": "2026-09-16T10:34:04.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A8-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A8-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A8-hrp", "action_params": {"source": "A1", "dest": "A8", "volume_ul": 12.121212121212121, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "volume_ul": 12.121212121212121, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-A8-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 87, "trace_sequence": 87, "timestamp": "2026-09-16T10:34:08+00:00", "logged_at": "2026-09-16T10:34:08.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A8-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A8-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A8-buffer", "action_params": {"source": "A3", "dest": "A8", "volume_ul": 179.87878787878788, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "volume_ul": 179.87878787878788, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-A8-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 88, "trace_sequence": 88, "timestamp": "2026-09-16T10:34:13+00:00", "logged_at": "2026-09-16T10:34:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A8-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-A8-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A8-tmb", "action_params": {"source": "A2", "dest": "A8", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-A8-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 89, "trace_sequence": 89, "timestamp": "2026-09-16T10:34:15+00:00", "logged_at": "2026-09-16T10:34:15.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-A8-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-A8-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-A8-mix", "action_params": {"well": "A8"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "enzyme_volume_ul": 12.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 179.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.2065090909090914, "rate_uM_per_s": 2.446969696969697, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 12.121212121212121, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-A8-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 90, "trace_sequence": 90, "timestamp": "2026-09-16T10:34:16+00:00", "logged_at": "2026-09-16T10:34:16.350000+00:00", "source_agent": null, "event_id": "obs-s8-A8-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A8-t3", "wells": {"plate:A8": {"enzyme_volume_ul": 12.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 179.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.2065090909090914, "rate_uM_per_s": 2.446969696969697, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 12.121212121212121, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.16032545454545458, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:34:13+00:00", "acquired_at": "2026-09-16T10:34:16+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03670454545454545}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 91, "trace_sequence": 91, "timestamp": "2026-09-16T10:34:19+00:00", "logged_at": "2026-09-16T10:34:19.350000+00:00", "source_agent": null, "event_id": "obs-s8-A8-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A8-t6", "wells": {"plate:A8": {"enzyme_volume_ul": 12.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 179.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.2065090909090914, "rate_uM_per_s": 2.446969696969697, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 12.121212121212121, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.32065090909090915, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:34:13+00:00", "acquired_at": "2026-09-16T10:34:19+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0734090909090909}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 92, "trace_sequence": 92, "timestamp": "2026-09-16T10:34:22+00:00", "logged_at": "2026-09-16T10:34:22.350000+00:00", "source_agent": null, "event_id": "obs-s8-A8-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-A8-t9", "wells": {"plate:A8": {"enzyme_volume_ul": 12.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 179.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.2065090909090914, "rate_uM_per_s": 2.446969696969697, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 12.121212121212121, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.4809763636363637, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:34:13+00:00", "acquired_at": "2026-09-16T10:34:22+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.11011363636363634}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 93, "trace_sequence": 93, "timestamp": "2026-09-16T10:34:35+00:00", "logged_at": "2026-09-16T10:34:35.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F1-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F1-hrp", "action_params": {"source": "H12", "dest": "F1", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F1", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-F1-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 94, "trace_sequence": 94, "timestamp": "2026-09-16T10:34:42+00:00", "logged_at": "2026-09-16T10:34:42.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F1-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F1-buffer", "action_params": {"source": "A3", "dest": "F1", "volume_ul": 188.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F1", "volume_ul": 188.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-F1-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 95, "trace_sequence": 95, "timestamp": "2026-09-16T10:34:51+00:00", "logged_at": "2026-09-16T10:34:51.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F1-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F1-tmb", "action_params": {"source": "A2", "dest": "F1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-F1-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 96, "trace_sequence": 96, "timestamp": "2026-09-16T10:34:52+00:00", "logged_at": "2026-09-16T10:34:52.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-F1-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F1-mix", "action_params": {"well": "F1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F1", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11499710413377459, "rate_uM_per_s": 0.08775725284933956, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-F1-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 97, "trace_sequence": 97, "timestamp": "2026-09-16T10:34:54+00:00", "logged_at": "2026-09-16T10:34:54.350000+00:00", "source_agent": null, "event_id": "obs-s8-F1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F1-t3", "wells": {"plate:F1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11499710413377459, "rate_uM_per_s": 0.08775725284933958, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.005749855206688729, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:34:51+00:00", "acquired_at": "2026-09-16T10:34:54+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01053087034192075}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 98, "trace_sequence": 98, "timestamp": "2026-09-16T10:34:57+00:00", "logged_at": "2026-09-16T10:34:57.350000+00:00", "source_agent": null, "event_id": "obs-s8-F1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F1-t6", "wells": {"plate:F1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11499710413377459, "rate_uM_per_s": 0.08775725284933958, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.011499710413377459, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:34:51+00:00", "acquired_at": "2026-09-16T10:34:57+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0210617406838415}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 99, "trace_sequence": 99, "timestamp": "2026-09-16T10:35:00+00:00", "logged_at": "2026-09-16T10:35:00.350000+00:00", "source_agent": null, "event_id": "obs-s8-F1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F1-t9", "wells": {"plate:F1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11499710413377459, "rate_uM_per_s": 0.08775725284933958, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.017249565620066187, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:34:51+00:00", "acquired_at": "2026-09-16T10:35:00+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.031592611025762246}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 100, "trace_sequence": 100, "timestamp": "2026-09-16T10:35:11+00:00", "logged_at": "2026-09-16T10:35:11.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G1-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G1-hrp", "action_params": {"source": "H12", "dest": "G1", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G1", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-G1-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 101, "trace_sequence": 101, "timestamp": "2026-09-16T10:35:18+00:00", "logged_at": "2026-09-16T10:35:18.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G1-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G1-buffer", "action_params": {"source": "A3", "dest": "G1", "volume_ul": 188.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G1", "volume_ul": 188.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-G1-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 102, "trace_sequence": 102, "timestamp": "2026-09-16T10:35:27+00:00", "logged_at": "2026-09-16T10:35:27.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G1-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G1-tmb", "action_params": {"source": "A2", "dest": "G1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-G1-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 103, "trace_sequence": 103, "timestamp": "2026-09-16T10:35:29+00:00", "logged_at": "2026-09-16T10:35:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-G1-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G1-mix", "action_params": {"well": "G1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G1", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11134471404804362, "rate_uM_per_s": 0.08497001987793316, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-G1-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 104, "trace_sequence": 104, "timestamp": "2026-09-16T10:35:30+00:00", "logged_at": "2026-09-16T10:35:30.350000+00:00", "source_agent": null, "event_id": "obs-s8-G1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G1-t3", "wells": {"plate:G1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11134471404804362, "rate_uM_per_s": 0.08497001987793316, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.005567235702402181, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:35:27+00:00", "acquired_at": "2026-09-16T10:35:30+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01019640238535198}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 105, "trace_sequence": 105, "timestamp": "2026-09-16T10:35:33+00:00", "logged_at": "2026-09-16T10:35:33.350000+00:00", "source_agent": null, "event_id": "obs-s8-G1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G1-t6", "wells": {"plate:G1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11134471404804362, "rate_uM_per_s": 0.08497001987793316, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.011134471404804362, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:35:27+00:00", "acquired_at": "2026-09-16T10:35:33+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.02039280477070396}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 106, "trace_sequence": 106, "timestamp": "2026-09-16T10:35:36+00:00", "logged_at": "2026-09-16T10:35:36.350000+00:00", "source_agent": null, "event_id": "obs-s8-G1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G1-t9", "wells": {"plate:G1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11134471404804362, "rate_uM_per_s": 0.08497001987793316, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.016701707107206542, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:35:27+00:00", "acquired_at": "2026-09-16T10:35:36+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03058920715605594}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 107, "trace_sequence": 107, "timestamp": "2026-09-16T10:35:50+00:00", "logged_at": "2026-09-16T10:35:50.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F2-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F2-hrp", "action_params": {"source": "H12", "dest": "F2", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F2", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-F2-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 108, "trace_sequence": 108, "timestamp": "2026-09-16T10:35:54+00:00", "logged_at": "2026-09-16T10:35:54.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F2-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F2-buffer", "action_params": {"source": "A3", "dest": "F2", "volume_ul": 187.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F2", "volume_ul": 187.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-F2-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 109, "trace_sequence": 109, "timestamp": "2026-09-16T10:35:59+00:00", "logged_at": "2026-09-16T10:35:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F2-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F2-tmb", "action_params": {"source": "A2", "dest": "F2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-F2-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 110, "trace_sequence": 110, "timestamp": "2026-09-16T10:36:01+00:00", "logged_at": "2026-09-16T10:36:01.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-F2-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F2-mix", "action_params": {"well": "F2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F2", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.1904443768610473, "rate_uM_per_s": 0.14533301042509714, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-F2-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 111, "trace_sequence": 111, "timestamp": "2026-09-16T10:36:02+00:00", "logged_at": "2026-09-16T10:36:02.350000+00:00", "source_agent": null, "event_id": "obs-s8-F2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F2-t3", "wells": {"plate:F2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.1904443768610473, "rate_uM_per_s": 0.1453330104250971, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.009522218843052365, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:35:59+00:00", "acquired_at": "2026-09-16T10:36:02+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008719980625505826}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 112, "trace_sequence": 112, "timestamp": "2026-09-16T10:36:05+00:00", "logged_at": "2026-09-16T10:36:05.350000+00:00", "source_agent": null, "event_id": "obs-s8-F2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F2-t6", "wells": {"plate:F2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.1904443768610473, "rate_uM_per_s": 0.1453330104250971, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.01904443768610473, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:35:59+00:00", "acquired_at": "2026-09-16T10:36:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.017439961251011653}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 113, "trace_sequence": 113, "timestamp": "2026-09-16T10:36:08+00:00", "logged_at": "2026-09-16T10:36:08.350000+00:00", "source_agent": null, "event_id": "obs-s8-F2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F2-t9", "wells": {"plate:F2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.1904443768610473, "rate_uM_per_s": 0.1453330104250971, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.028566656529157092, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:35:59+00:00", "acquired_at": "2026-09-16T10:36:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.02615994187651748}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 114, "trace_sequence": 114, "timestamp": "2026-09-16T10:36:17+00:00", "logged_at": "2026-09-16T10:36:17.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G2-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G2-hrp", "action_params": {"source": "H12", "dest": "G2", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G2", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-G2-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 115, "trace_sequence": 115, "timestamp": "2026-09-16T10:36:23+00:00", "logged_at": "2026-09-16T10:36:23.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G2-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G2-buffer", "action_params": {"source": "A3", "dest": "G2", "volume_ul": 187.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G2", "volume_ul": 187.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-G2-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 116, "trace_sequence": 116, "timestamp": "2026-09-16T10:36:28+00:00", "logged_at": "2026-09-16T10:36:28.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G2-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G2-tmb", "action_params": {"source": "A2", "dest": "G2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-G2-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 117, "trace_sequence": 117, "timestamp": "2026-09-16T10:36:29+00:00", "logged_at": "2026-09-16T10:36:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-G2-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G2-mix", "action_params": {"well": "G2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G2", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.18679198677531633, "rate_uM_per_s": 0.1425457774536907, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-G2-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 118, "trace_sequence": 118, "timestamp": "2026-09-16T10:36:31+00:00", "logged_at": "2026-09-16T10:36:31.350000+00:00", "source_agent": null, "event_id": "obs-s8-G2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G2-t3", "wells": {"plate:G2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.18679198677531633, "rate_uM_per_s": 0.1425457774536907, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.009339599338765818, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:36:28+00:00", "acquired_at": "2026-09-16T10:36:31+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008552746647221443}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 119, "trace_sequence": 119, "timestamp": "2026-09-16T10:36:34+00:00", "logged_at": "2026-09-16T10:36:34.350000+00:00", "source_agent": null, "event_id": "obs-s8-G2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G2-t6", "wells": {"plate:G2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.18679198677531633, "rate_uM_per_s": 0.1425457774536907, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.018679198677531636, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:36:28+00:00", "acquired_at": "2026-09-16T10:36:34+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.017105493294442886}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 120, "trace_sequence": 120, "timestamp": "2026-09-16T10:36:37+00:00", "logged_at": "2026-09-16T10:36:37.350000+00:00", "source_agent": null, "event_id": "obs-s8-G2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G2-t9", "wells": {"plate:G2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.18679198677531633, "rate_uM_per_s": 0.1425457774536907, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.02801879801629745, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:36:28+00:00", "acquired_at": "2026-09-16T10:36:37+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.025658239941664326}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 121, "trace_sequence": 121, "timestamp": "2026-09-16T10:36:49+00:00", "logged_at": "2026-09-16T10:36:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F3-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F3-hrp", "action_params": {"source": "H12", "dest": "F3", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F3", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-F3-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 122, "trace_sequence": 122, "timestamp": "2026-09-16T10:36:55+00:00", "logged_at": "2026-09-16T10:36:55.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F3-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F3-buffer", "action_params": {"source": "A3", "dest": "F3", "volume_ul": 185.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F3", "volume_ul": 185.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-F3-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 123, "trace_sequence": 123, "timestamp": "2026-09-16T10:37:02+00:00", "logged_at": "2026-09-16T10:37:02.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F3-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F3-tmb", "action_params": {"source": "A2", "dest": "F3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-F3-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 124, "trace_sequence": 124, "timestamp": "2026-09-16T10:37:04+00:00", "logged_at": "2026-09-16T10:37:04.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-F3-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F3-mix", "action_params": {"well": "F3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F3", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.2847534677701382, "rate_uM_per_s": 0.21730270739479413, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-F3-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 125, "trace_sequence": 125, "timestamp": "2026-09-16T10:37:05+00:00", "logged_at": "2026-09-16T10:37:05.350000+00:00", "source_agent": null, "event_id": "obs-s8-F3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F3-t3", "wells": {"plate:F3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.2847534677701382, "rate_uM_per_s": 0.21730270739479407, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.01423767338850691, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:37:02+00:00", "acquired_at": "2026-09-16T10:37:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.006519081221843822}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 126, "trace_sequence": 126, "timestamp": "2026-09-16T10:37:08+00:00", "logged_at": "2026-09-16T10:37:08.350000+00:00", "source_agent": null, "event_id": "obs-s8-F3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F3-t6", "wells": {"plate:F3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.2847534677701382, "rate_uM_per_s": 0.21730270739479407, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.02847534677701382, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:37:02+00:00", "acquired_at": "2026-09-16T10:37:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.013038162443687645}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 127, "trace_sequence": 127, "timestamp": "2026-09-16T10:37:11+00:00", "logged_at": "2026-09-16T10:37:11.350000+00:00", "source_agent": null, "event_id": "obs-s8-F3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F3-t9", "wells": {"plate:F3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.2847534677701382, "rate_uM_per_s": 0.21730270739479407, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.04271302016552073, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:37:02+00:00", "acquired_at": "2026-09-16T10:37:11+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.019557243665531465}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 128, "trace_sequence": 128, "timestamp": "2026-09-16T10:37:19+00:00", "logged_at": "2026-09-16T10:37:19.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G3-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G3-hrp", "action_params": {"source": "H12", "dest": "G3", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G3", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-G3-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 129, "trace_sequence": 129, "timestamp": "2026-09-16T10:37:26+00:00", "logged_at": "2026-09-16T10:37:26.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G3-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G3-buffer", "action_params": {"source": "A3", "dest": "G3", "volume_ul": 185.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G3", "volume_ul": 185.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-G3-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 130, "trace_sequence": 130, "timestamp": "2026-09-16T10:37:30+00:00", "logged_at": "2026-09-16T10:37:30.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G3-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G3-tmb", "action_params": {"source": "A2", "dest": "G3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-G3-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 131, "trace_sequence": 131, "timestamp": "2026-09-16T10:37:32+00:00", "logged_at": "2026-09-16T10:37:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-G3-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G3-mix", "action_params": {"well": "G3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G3", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.28110107768440723, "rate_uM_per_s": 0.2145154744233877, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-G3-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 132, "trace_sequence": 132, "timestamp": "2026-09-16T10:37:33+00:00", "logged_at": "2026-09-16T10:37:33.350000+00:00", "source_agent": null, "event_id": "obs-s8-G3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G3-t3", "wells": {"plate:G3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.28110107768440723, "rate_uM_per_s": 0.21451547442338767, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.014055053884220363, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:37:30+00:00", "acquired_at": "2026-09-16T10:37:33+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00643546423270163}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 133, "trace_sequence": 133, "timestamp": "2026-09-16T10:37:36+00:00", "logged_at": "2026-09-16T10:37:36.350000+00:00", "source_agent": null, "event_id": "obs-s8-G3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G3-t6", "wells": {"plate:G3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.28110107768440723, "rate_uM_per_s": 0.21451547442338767, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.028110107768440726, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:37:30+00:00", "acquired_at": "2026-09-16T10:37:36+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01287092846540326}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 134, "trace_sequence": 134, "timestamp": "2026-09-16T10:37:39+00:00", "logged_at": "2026-09-16T10:37:39.350000+00:00", "source_agent": null, "event_id": "obs-s8-G3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G3-t9", "wells": {"plate:G3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.28110107768440723, "rate_uM_per_s": 0.21451547442338767, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.042165161652661085, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:37:30+00:00", "acquired_at": "2026-09-16T10:37:39+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.019306392698104892}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 135, "trace_sequence": 135, "timestamp": "2026-09-16T10:37:54+00:00", "logged_at": "2026-09-16T10:37:54.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F4-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F4-hrp", "action_params": {"source": "H12", "dest": "F4", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F4", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-F4-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 136, "trace_sequence": 136, "timestamp": "2026-09-16T10:37:59+00:00", "logged_at": "2026-09-16T10:37:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F4-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F4-buffer", "action_params": {"source": "A3", "dest": "F4", "volume_ul": 181.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F4", "volume_ul": 181.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-F4-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 137, "trace_sequence": 137, "timestamp": "2026-09-16T10:38:06+00:00", "logged_at": "2026-09-16T10:38:06.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F4-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F4-tmb", "action_params": {"source": "A2", "dest": "F4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-F4-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 138, "trace_sequence": 138, "timestamp": "2026-09-16T10:38:07+00:00", "logged_at": "2026-09-16T10:38:07.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-F4-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F4-mix", "action_params": {"well": "F4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F4", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.37906255867922906, "rate_uM_per_s": 0.28927240436449103, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-F4-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 139, "trace_sequence": 139, "timestamp": "2026-09-16T10:38:09+00:00", "logged_at": "2026-09-16T10:38:09.350000+00:00", "source_agent": null, "event_id": "obs-s8-F4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F4-t3", "wells": {"plate:F4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.37906255867922906, "rate_uM_per_s": 0.28927240436449103, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.018953127933961453, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:38:06+00:00", "acquired_at": "2026-09-16T10:38:09+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.004339086065467366}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 140, "trace_sequence": 140, "timestamp": "2026-09-16T10:38:12+00:00", "logged_at": "2026-09-16T10:38:12.350000+00:00", "source_agent": null, "event_id": "obs-s8-F4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F4-t6", "wells": {"plate:F4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.37906255867922906, "rate_uM_per_s": 0.28927240436449103, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.03790625586792291, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:38:06+00:00", "acquired_at": "2026-09-16T10:38:12+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008678172130934731}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 141, "trace_sequence": 141, "timestamp": "2026-09-16T10:38:15+00:00", "logged_at": "2026-09-16T10:38:15.350000+00:00", "source_agent": null, "event_id": "obs-s8-F4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F4-t9", "wells": {"plate:F4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.37906255867922906, "rate_uM_per_s": 0.28927240436449103, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.05685938380188436, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:38:06+00:00", "acquired_at": "2026-09-16T10:38:15+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.013017258196402098}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 142, "trace_sequence": 142, "timestamp": "2026-09-16T10:38:30+00:00", "logged_at": "2026-09-16T10:38:30.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G4-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G4-hrp", "action_params": {"source": "H12", "dest": "G4", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G4", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-G4-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 143, "trace_sequence": 143, "timestamp": "2026-09-16T10:38:36+00:00", "logged_at": "2026-09-16T10:38:36.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G4-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G4-buffer", "action_params": {"source": "A3", "dest": "G4", "volume_ul": 181.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G4", "volume_ul": 181.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-G4-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 144, "trace_sequence": 144, "timestamp": "2026-09-16T10:38:40+00:00", "logged_at": "2026-09-16T10:38:40.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G4-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G4-tmb", "action_params": {"source": "A2", "dest": "G4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-G4-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 145, "trace_sequence": 145, "timestamp": "2026-09-16T10:38:41+00:00", "logged_at": "2026-09-16T10:38:41.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-G4-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G4-mix", "action_params": {"well": "G4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G4", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3754101685934982, "rate_uM_per_s": 0.28648517139308466, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-G4-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 146, "trace_sequence": 146, "timestamp": "2026-09-16T10:38:43+00:00", "logged_at": "2026-09-16T10:38:43.350000+00:00", "source_agent": null, "event_id": "obs-s8-G4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G4-t3", "wells": {"plate:G4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3754101685934982, "rate_uM_per_s": 0.28648517139308466, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.01877050842967491, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:38:40+00:00", "acquired_at": "2026-09-16T10:38:43+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00429727757089627}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 147, "trace_sequence": 147, "timestamp": "2026-09-16T10:38:46+00:00", "logged_at": "2026-09-16T10:38:46.350000+00:00", "source_agent": null, "event_id": "obs-s8-G4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G4-t6", "wells": {"plate:G4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3754101685934982, "rate_uM_per_s": 0.28648517139308466, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.03754101685934982, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:38:40+00:00", "acquired_at": "2026-09-16T10:38:46+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00859455514179254}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 148, "trace_sequence": 148, "timestamp": "2026-09-16T10:38:49+00:00", "logged_at": "2026-09-16T10:38:49.350000+00:00", "source_agent": null, "event_id": "obs-s8-G4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G4-t9", "wells": {"plate:G4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3754101685934982, "rate_uM_per_s": 0.28648517139308466, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.05631152528902473, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:38:40+00:00", "acquired_at": "2026-09-16T10:38:49+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01289183271268881}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 149, "trace_sequence": 149, "timestamp": "2026-09-16T10:39:01+00:00", "logged_at": "2026-09-16T10:39:01.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F5-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F5-hrp", "action_params": {"source": "H12", "dest": "F5", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F5", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-F5-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 150, "trace_sequence": 150, "timestamp": "2026-09-16T10:39:09+00:00", "logged_at": "2026-09-16T10:39:09.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F5-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F5-buffer", "action_params": {"source": "A3", "dest": "F5", "volume_ul": 173.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F5", "volume_ul": 173.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-F5-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 151, "trace_sequence": 151, "timestamp": "2026-09-16T10:39:13+00:00", "logged_at": "2026-09-16T10:39:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-F5-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F5-tmb", "action_params": {"source": "A2", "dest": "F5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-F5-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 152, "trace_sequence": 152, "timestamp": "2026-09-16T10:39:15+00:00", "logged_at": "2026-09-16T10:39:15.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-F5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-F5-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-F5-mix", "action_params": {"well": "F5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:F5", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45450983140650186, "rate_uM_per_s": 0.34684816194024864, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-F5-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 153, "trace_sequence": 153, "timestamp": "2026-09-16T10:39:16+00:00", "logged_at": "2026-09-16T10:39:16.350000+00:00", "source_agent": null, "event_id": "obs-s8-F5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F5-t3", "wells": {"plate:F5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45450983140650186, "rate_uM_per_s": 0.3468481619402486, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.022725491570325093, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:39:13+00:00", "acquired_at": "2026-09-16T10:39:16+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0026013612145518645}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 154, "trace_sequence": 154, "timestamp": "2026-09-16T10:39:19+00:00", "logged_at": "2026-09-16T10:39:19.350000+00:00", "source_agent": null, "event_id": "obs-s8-F5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F5-t6", "wells": {"plate:F5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45450983140650186, "rate_uM_per_s": 0.3468481619402486, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.045450983140650186, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:39:13+00:00", "acquired_at": "2026-09-16T10:39:19+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005202722429103729}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 155, "trace_sequence": 155, "timestamp": "2026-09-16T10:39:22+00:00", "logged_at": "2026-09-16T10:39:22.350000+00:00", "source_agent": null, "event_id": "obs-s8-F5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-F5-t9", "wells": {"plate:F5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45450983140650186, "rate_uM_per_s": 0.3468481619402486, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.06817647471097528, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:39:13+00:00", "acquired_at": "2026-09-16T10:39:22+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.007804083643655593}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 156, "trace_sequence": 156, "timestamp": "2026-09-16T10:39:33+00:00", "logged_at": "2026-09-16T10:39:33.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G5-hrp", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G5-hrp", "action_params": {"source": "H12", "dest": "G5", "volume_ul": 10.606060606060606, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G5", "volume_ul": 10.606060606060606, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s8-diluted-G5-hrp"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 157, "trace_sequence": 157, "timestamp": "2026-09-16T10:39:41+00:00", "logged_at": "2026-09-16T10:39:41.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G5-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G5-buffer", "action_params": {"source": "A3", "dest": "G5", "volume_ul": 173.3939393939394, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G5", "volume_ul": 173.3939393939394, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-G5-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 158, "trace_sequence": 158, "timestamp": "2026-09-16T10:39:50+00:00", "logged_at": "2026-09-16T10:39:50.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-G5-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G5-tmb", "action_params": {"source": "A2", "dest": "G5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-G5-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 159, "trace_sequence": 159, "timestamp": "2026-09-16T10:39:52+00:00", "logged_at": "2026-09-16T10:39:52.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-G5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-G5-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-G5-mix", "action_params": {"well": "G5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:G5", "enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45085744132077094, "rate_uM_per_s": 0.3440609289688423, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}}, "result_observation_ids": ["obs-plan-s8-diluted-G5-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 160, "trace_sequence": 160, "timestamp": "2026-09-16T10:39:53+00:00", "logged_at": "2026-09-16T10:39:53.350000+00:00", "source_agent": null, "event_id": "obs-s8-G5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G5-t3", "wells": {"plate:G5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45085744132077094, "rate_uM_per_s": 0.3440609289688422, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.022542872066038548, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:39:50+00:00", "acquired_at": "2026-09-16T10:39:53+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.002580456967266317}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 161, "trace_sequence": 161, "timestamp": "2026-09-16T10:39:56+00:00", "logged_at": "2026-09-16T10:39:56.350000+00:00", "source_agent": null, "event_id": "obs-s8-G5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G5-t6", "wells": {"plate:G5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45085744132077094, "rate_uM_per_s": 0.3440609289688422, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.045085744132077096, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:39:50+00:00", "acquired_at": "2026-09-16T10:39:56+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005160913934532634}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 162, "trace_sequence": 162, "timestamp": "2026-09-16T10:39:59+00:00", "logged_at": "2026-09-16T10:39:59.350000+00:00", "source_agent": null, "event_id": "obs-s8-G5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-G5-t9", "wells": {"plate:G5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45085744132077094, "rate_uM_per_s": 0.3440609289688422, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock", "A652": 0.06762861619811564, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:39:50+00:00", "acquired_at": "2026-09-16T10:39:59+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00774137090179895}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 163, "trace_sequence": 163, "timestamp": "2026-09-16T10:40:07+00:00", "logged_at": "2026-09-16T10:40:07.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E1-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E1-buffer", "action_params": {"source": "A3", "dest": "E1", "volume_ul": 199.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E1", "volume_ul": 199.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-E1-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 164, "trace_sequence": 164, "timestamp": "2026-09-16T10:40:11+00:00", "logged_at": "2026-09-16T10:40:11.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E1-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E1-tmb", "action_params": {"source": "A2", "dest": "E1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-E1-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 165, "trace_sequence": 165, "timestamp": "2026-09-16T10:40:13+00:00", "logged_at": "2026-09-16T10:40:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-E1-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E1-mix", "action_params": {"well": "E1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E1", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0}}, "result_observation_ids": ["obs-plan-s8-diluted-E1-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 166, "trace_sequence": 166, "timestamp": "2026-09-16T10:40:14+00:00", "logged_at": "2026-09-16T10:40:14.350000+00:00", "source_agent": null, "event_id": "obs-s8-E1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E1-t3", "wells": {"plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:40:11+00:00", "acquired_at": "2026-09-16T10:40:14+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 167, "trace_sequence": 167, "timestamp": "2026-09-16T10:40:17+00:00", "logged_at": "2026-09-16T10:40:17.350000+00:00", "source_agent": null, "event_id": "obs-s8-E1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E1-t6", "wells": {"plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:40:11+00:00", "acquired_at": "2026-09-16T10:40:17+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 168, "trace_sequence": 168, "timestamp": "2026-09-16T10:40:20+00:00", "logged_at": "2026-09-16T10:40:20.350000+00:00", "source_agent": null, "event_id": "obs-s8-E1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E1-t9", "wells": {"plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:40:11+00:00", "acquired_at": "2026-09-16T10:40:20+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 169, "trace_sequence": 169, "timestamp": "2026-09-16T10:40:36+00:00", "logged_at": "2026-09-16T10:40:36.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E2-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E2-buffer", "action_params": {"source": "A3", "dest": "E2", "volume_ul": 198.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E2", "volume_ul": 198.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-E2-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 170, "trace_sequence": 170, "timestamp": "2026-09-16T10:40:41+00:00", "logged_at": "2026-09-16T10:40:41.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E2-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E2-tmb", "action_params": {"source": "A2", "dest": "E2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-E2-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 171, "trace_sequence": 171, "timestamp": "2026-09-16T10:40:43+00:00", "logged_at": "2026-09-16T10:40:43.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-E2-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E2-mix", "action_params": {"well": "E2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E2", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0}}, "result_observation_ids": ["obs-plan-s8-diluted-E2-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 172, "trace_sequence": 172, "timestamp": "2026-09-16T10:40:44+00:00", "logged_at": "2026-09-16T10:40:44.350000+00:00", "source_agent": null, "event_id": "obs-s8-E2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E2-t3", "wells": {"plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:40:41+00:00", "acquired_at": "2026-09-16T10:40:44+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 173, "trace_sequence": 173, "timestamp": "2026-09-16T10:40:47+00:00", "logged_at": "2026-09-16T10:40:47.350000+00:00", "source_agent": null, "event_id": "obs-s8-E2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E2-t6", "wells": {"plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:40:41+00:00", "acquired_at": "2026-09-16T10:40:47+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 174, "trace_sequence": 174, "timestamp": "2026-09-16T10:40:50+00:00", "logged_at": "2026-09-16T10:40:50.350000+00:00", "source_agent": null, "event_id": "obs-s8-E2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E2-t9", "wells": {"plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:40:41+00:00", "acquired_at": "2026-09-16T10:40:50+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 175, "trace_sequence": 175, "timestamp": "2026-09-16T10:41:01+00:00", "logged_at": "2026-09-16T10:41:01.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E3-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E3-buffer", "action_params": {"source": "A3", "dest": "E3", "volume_ul": 196.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E3", "volume_ul": 196.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-E3-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 176, "trace_sequence": 176, "timestamp": "2026-09-16T10:41:05+00:00", "logged_at": "2026-09-16T10:41:05.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E3-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E3-tmb", "action_params": {"source": "A2", "dest": "E3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-E3-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 177, "trace_sequence": 177, "timestamp": "2026-09-16T10:41:06+00:00", "logged_at": "2026-09-16T10:41:06.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-E3-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E3-mix", "action_params": {"well": "E3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E3", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0}}, "result_observation_ids": ["obs-plan-s8-diluted-E3-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 178, "trace_sequence": 178, "timestamp": "2026-09-16T10:41:08+00:00", "logged_at": "2026-09-16T10:41:08.350000+00:00", "source_agent": null, "event_id": "obs-s8-E3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E3-t3", "wells": {"plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:41:05+00:00", "acquired_at": "2026-09-16T10:41:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 179, "trace_sequence": 179, "timestamp": "2026-09-16T10:41:11+00:00", "logged_at": "2026-09-16T10:41:11.350000+00:00", "source_agent": null, "event_id": "obs-s8-E3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E3-t6", "wells": {"plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:41:05+00:00", "acquired_at": "2026-09-16T10:41:11+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 180, "trace_sequence": 180, "timestamp": "2026-09-16T10:41:14+00:00", "logged_at": "2026-09-16T10:41:14.350000+00:00", "source_agent": null, "event_id": "obs-s8-E3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E3-t9", "wells": {"plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:41:05+00:00", "acquired_at": "2026-09-16T10:41:14+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 181, "trace_sequence": 181, "timestamp": "2026-09-16T10:41:27+00:00", "logged_at": "2026-09-16T10:41:27.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E4-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E4-buffer", "action_params": {"source": "A3", "dest": "E4", "volume_ul": 192.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E4", "volume_ul": 192.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-E4-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 182, "trace_sequence": 182, "timestamp": "2026-09-16T10:41:34+00:00", "logged_at": "2026-09-16T10:41:34.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E4-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E4-tmb", "action_params": {"source": "A2", "dest": "E4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-E4-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 183, "trace_sequence": 183, "timestamp": "2026-09-16T10:41:35+00:00", "logged_at": "2026-09-16T10:41:35.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-E4-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E4-mix", "action_params": {"well": "E4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E4", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0}}, "result_observation_ids": ["obs-plan-s8-diluted-E4-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 184, "trace_sequence": 184, "timestamp": "2026-09-16T10:41:37+00:00", "logged_at": "2026-09-16T10:41:37.350000+00:00", "source_agent": null, "event_id": "obs-s8-E4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E4-t3", "wells": {"plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:41:34+00:00", "acquired_at": "2026-09-16T10:41:37+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 185, "trace_sequence": 185, "timestamp": "2026-09-16T10:41:40+00:00", "logged_at": "2026-09-16T10:41:40.350000+00:00", "source_agent": null, "event_id": "obs-s8-E4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E4-t6", "wells": {"plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:41:34+00:00", "acquired_at": "2026-09-16T10:41:40+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 186, "trace_sequence": 186, "timestamp": "2026-09-16T10:41:43+00:00", "logged_at": "2026-09-16T10:41:43.350000+00:00", "source_agent": null, "event_id": "obs-s8-E4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E4-t9", "wells": {"plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:41:34+00:00", "acquired_at": "2026-09-16T10:41:43+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 187, "trace_sequence": 187, "timestamp": "2026-09-16T10:41:56+00:00", "logged_at": "2026-09-16T10:41:56.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E5-buffer", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E5-buffer", "action_params": {"source": "A3", "dest": "E5", "volume_ul": 184.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E5", "volume_ul": 184.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s8-diluted-E5-buffer"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 188, "trace_sequence": 188, "timestamp": "2026-09-16T10:42:00+00:00", "logged_at": "2026-09-16T10:42:00.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s8-diluted-E5-tmb", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E5-tmb", "action_params": {"source": "A2", "dest": "E5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s8-diluted-E5-tmb"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 189, "trace_sequence": 189, "timestamp": "2026-09-16T10:42:01+00:00", "logged_at": "2026-09-16T10:42:01.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s8-diluted-E5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s8-diluted-E5-mix", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s8-diluted-E5-mix", "action_params": {"well": "E5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E5", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0}}, "result_observation_ids": ["obs-plan-s8-diluted-E5-mix"], "state_delta": {"execution_disposition": "dispatched"}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 190, "trace_sequence": 190, "timestamp": "2026-09-16T10:42:03+00:00", "logged_at": "2026-09-16T10:42:03.350000+00:00", "source_agent": null, "event_id": "obs-s8-E5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E5-t3", "wells": {"plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:42:00+00:00", "acquired_at": "2026-09-16T10:42:03+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 191, "trace_sequence": 191, "timestamp": "2026-09-16T10:42:06+00:00", "logged_at": "2026-09-16T10:42:06.350000+00:00", "source_agent": null, "event_id": "obs-s8-E5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E5-t6", "wells": {"plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:42:00+00:00", "acquired_at": "2026-09-16T10:42:06+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 192, "trace_sequence": 192, "timestamp": "2026-09-16T10:42:09+00:00", "logged_at": "2026-09-16T10:42:09.350000+00:00", "source_agent": null, "event_id": "obs-s8-E5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-E5-t9", "wells": {"plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:42:00+00:00", "acquired_at": "2026-09-16T10:42:09+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 193, "trace_sequence": 193, "timestamp": "2026-09-16T10:42:21+00:00", "logged_at": "2026-09-16T10:42:21.350000+00:00", "source_agent": null, "event_id": "obs-s8-fresh-composition", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s8-fresh-composition", "wells": {"plate:A1": {"enzyme_volume_ul": 1.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "rate_A652_per_min": 0.03772363636363637, "rate_uM_per_s": 0.028787878787878782, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.07575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A2": {"enzyme_volume_ul": 2.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 189.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07544727272727274, "rate_uM_per_s": 0.057575757575757565, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.15151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A3": {"enzyme_volume_ul": 4.242424242424242, "tmb_volume_ul": 8.0, "buffer_volume_ul": 187.75757575757575, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15089454545454548, "rate_uM_per_s": 0.11515151515151513, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.30303030303030304, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3772363636363637, "rate_uM_per_s": 0.2878787878787879, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A5": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.712557575757576, "rate_uM_per_s": 0.5437710437710438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A6": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.2826036363636366, "rate_uM_per_s": 0.9787878787878789, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A7": {"enzyme_volume_ul": 6.0606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 185.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.1376727272727276, "rate_uM_per_s": 1.6313131313131313, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 6.0606060606060606, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:A8": {"enzyme_volume_ul": 12.121212121212121, "tmb_volume_ul": 8.0, "buffer_volume_ul": 179.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.2065090909090914, "rate_uM_per_s": 2.446969696969697, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 12.121212121212121, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:F1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11499710413377459, "rate_uM_per_s": 0.08775725284933956, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:G1": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 1.0, "buffer_volume_ul": 188.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.11134471404804362, "rate_uM_per_s": 0.08497001987793316, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.08636363636363636, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:F2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.1904443768610473, "rate_uM_per_s": 0.14533301042509714, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:G2": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 2.0, "buffer_volume_ul": 187.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.18679198677531633, "rate_uM_per_s": 0.1425457774536907, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.14393939393939392, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:F3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.2847534677701382, "rate_uM_per_s": 0.21730270739479413, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:G3": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 4.0, "buffer_volume_ul": 185.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.28110107768440723, "rate_uM_per_s": 0.2145154744233877, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.2159090909090909, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:F4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.37906255867922906, "rate_uM_per_s": 0.28927240436449103, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:G4": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.3754101685934982, "rate_uM_per_s": 0.28648517139308466, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.28787878787878785, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:F5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45450983140650186, "rate_uM_per_s": 0.34684816194024864, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": 0.0013936164857032004, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:G5": {"enzyme_volume_ul": 10.606060606060606, "tmb_volume_ul": 16.0, "buffer_volume_ul": 173.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.45085744132077094, "rate_uM_per_s": 0.3440609289688423, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 14, "neat_enzyme_ul": 0.7575757575757576, "rate_uM_per_s_mean": 0.34545454545454546, "rate_residual_uM_per_s": -0.0013936164857032004, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "concentration_basis": "exact_stock"}, "plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0}, "plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0}, "plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0}, "plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0}, "plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0}}, "note": "Compositions from the given 30 nM stock and the volumes dispensed."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 194, "trace_sequence": 194, "timestamp": "2026-09-16T10:42:21+00:00", "logged_at": "2026-09-16T10:42:21.350000+00:00", "source_agent": "human", "event_id": "s8-audit", "event_type": "audit_annotation", "observation_type": "audit_annotation", "message": "Prepared concentrations from the given 30 nM stock. Not shown to the agent.", "payload": {"disclosed_to_agents": false, "wells": {"plate:B1": {"physical_enzyme_ng_ml": 100.0, "physical_enzyme_nM": 2.272727272727273}, "plate:B2": {"physical_enzyme_ng_ml": 100.0, "physical_enzyme_nM": 2.272727272727273}, "plate:B3": {"physical_enzyme_ng_ml": 100.0, "physical_enzyme_nM": 2.272727272727273}, "plate:B4": {"physical_enzyme_ng_ml": 100.0, "physical_enzyme_nM": 2.272727272727273}, "plate:B5": {"physical_enzyme_ng_ml": 100.0, "physical_enzyme_nM": 2.272727272727273}, "plate:A1": {"physical_enzyme_ng_ml": 0.5, "physical_enzyme_nM": 0.011363636363636362}, "plate:A2": {"physical_enzyme_ng_ml": 1.0, "physical_enzyme_nM": 0.022727272727272724}, "plate:A3": {"physical_enzyme_ng_ml": 2.0, "physical_enzyme_nM": 0.04545454545454545}, "plate:A4": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:A5": {"physical_enzyme_ng_ml": 10.0, "physical_enzyme_nM": 0.22727272727272727}, "plate:A6": {"physical_enzyme_ng_ml": 20.0, "physical_enzyme_nM": 0.45454545454545453}, "plate:A7": {"physical_enzyme_ng_ml": 40.0, "physical_enzyme_nM": 0.9090909090909091}, "plate:A8": {"physical_enzyme_ng_ml": 80.0, "physical_enzyme_nM": 1.8181818181818181}, "plate:F1": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:G1": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:F2": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:G2": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:F3": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:G3": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:F4": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:G4": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:F5": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}, "plate:G5": {"physical_enzyme_ng_ml": 5.0, "physical_enzyme_nM": 0.11363636363636363}}, "note": "Prepared concentrations from the given 30 nM stock. Not shown to the agent."}}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 195, "trace_sequence": 195, "timestamp": "2026-09-16T10:42:33+00:00", "logged_at": "2026-09-16T10:42:33.350000+00:00", "source_agent": "planner", "event_id": "msg-0195", "event_type": "recovery_decision", "rationale": "Reported KM = 100 uM. kcat = Vmax/[E] = 3800 /s at 5 ng/mL (0.1136 nM) HRP. The points that stayed proportional are 0.5, 1, 2, and 5 ng/mL. Observations obs-s8-A1-t9, obs-s8-A2-t9, obs-s8-A3-t9, and obs-s8-A4-t9 define that linear working range. obs-s8-A5-t9 is outside it. The given stock is 30 nM, which is 1320 ng/mL. 15.1515 uL of that stock in 200 uL was a separate saturated preparation at 100 ng/mL. The new wells, including the adopted well, are new reactions made from the H12 aliquot, then PBS and TMB. They are not a transfer of that saturated reaction. H12 is a 14-fold dilution, 20 uL of the 30 nM stock plus 260 uL PBS. The adopted well takes 10.6061 uL of that aliquot in 200 uL, which is 5 ng/mL. That 5 ng/mL is the concentration those volumes produce, and reported kcat uses it. Vmax is 0.4318 uM/s, converted from 0.5659 A652/min with epsilon 39000 /M/cm and path 0.56 cm. That path is 0.28 cm times the well volume divided by 100 uL, which is 0.56 cm at 200 uL. The linear HRP working range is 0.5-5 ng/mL.", "message": "Reported KM = 100 uM. kcat = Vmax/[E] = 3800 /s at 5 ng/mL (0.1136 nM) HRP. The points that stayed proportional are 0.5, 1, 2, and 5 ng/mL. Observations obs-s8-A1-t9, obs-s8-A2-t9, obs-s8-A3-t9, and obs-s8-A4-t9 define that linear working range. obs-s8-A5-t9 is outside it. The given stock is 30 nM, which is 1320 ng/mL. 15.1515 uL of that stock in 200 uL was a separate saturated preparation at 100 ng/mL. The new wells, including the adopted well, are new reactions made from the H12 aliquot, then PBS and TMB. They are not a transfer of that saturated reaction. H12 is a 14-fold dilution, 20 uL of the 30 nM stock plus 260 uL PBS. The adopted well takes 10.6061 uL of that aliquot in 200 uL, which is 5 ng/mL. That 5 ng/mL is the concentration those volumes produce, and reported kcat uses it. Vmax is 0.4318 uM/s, converted from 0.5659 A652/min with epsilon 39000 /M/cm and path 0.56 cm. That path is 0.28 cm times the well volume divided by 100 uL, which is 0.56 cm at 200 uL. The linear HRP working range is 0.5-5 ng/mL."}
{"record_kind": "event", "run_id": "pearl-manuscript-s8-20260916", "seq": 196, "trace_sequence": 196, "timestamp": "2026-09-16T10:42:45+00:00", "logged_at": "2026-09-16T10:42:45.350000+00:00", "source_agent": "planner", "event_id": "done-S8", "event_type": "completion_decision", "plan_id": "plan-s8-diluted", "revision_id": "rev_0", "adopted": {"summary": "Working range, KM, and kcat after the dilution search.", "KM_uM": 100.0, "kcat_per_s": 3800.0, "Vmax_M_per_s": 4.318181818181818e-07, "Vmax_uM_per_s": 0.4318181818181818, "Vmax_A652_per_min": 0.5658545454545455, "enzyme_concentration_M": 1.1363636363636364e-10, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "basis": "kcat_per_s = Vmax_M_per_s / enzyme_concentration_M", "read_mode": "same_well", "acid_stop": false, "enzyme_stock": "30 nM, 2000 uL", "working_range_analyte": "HRP", "linear_working_range_hrp_ng_ml": [0.5, 5.0], "linear_working_range_hrp_nM": [0.011363636363636364, 0.11363636363636363], "preparation_id": "prep-s8-diluted", "concentration_assigned_by": "dilution_of_given_stock", "dilution_chosen_by": "dilution_search", "aliquot_dilution_factor": 14.0, "aliquot_stock_ul": 20.0, "aliquot_buffer_ul": 260.0, "aliquot_well": "H12", "adopted_well_is_new_reaction": true, "reference_protocol_id": "protocol-hrp-tmb-initial-rate-curve", "reference_event_id": "know-protocol-hrp-tmb-initial-rate-curve", "first_enzyme_ng_ml": 100.0, "first_enzyme_volume_ul": 15.151515151515152, "adopted_enzyme_ng_ml": 5.0, "adopted_enzyme_volume_ul": 10.606060606060606, "adopted_enzyme_source": "H12", "dilution_prescribed": false}, "discarded": ["saturated first attempt"], "message": "Working range, KM, and kcat after the dilution search."}
{"record_kind": "trial", "run_id": "pearl-manuscript-s8-20260916", "seq": 197, "trace_sequence": 197, "timestamp": "2026-09-16T10:42:57+00:00", "logged_at": "2026-09-16T10:42:57.350000+00:00", "source_agent": null, "scenario_id": "S8", "termination": "completed", "not_wet_lab": false, "adopted": {"summary": "Working range, KM, and kcat after the dilution search.", "KM_uM": 100.0, "kcat_per_s": 3800.0, "Vmax_M_per_s": 4.318181818181818e-07, "Vmax_uM_per_s": 0.4318181818181818, "Vmax_A652_per_min": 0.5658545454545455, "enzyme_concentration_M": 1.1363636363636364e-10, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "basis": "kcat_per_s = Vmax_M_per_s / enzyme_concentration_M", "read_mode": "same_well", "acid_stop": false, "enzyme_stock": "30 nM, 2000 uL", "working_range_analyte": "HRP", "linear_working_range_hrp_ng_ml": [0.5, 5.0], "linear_working_range_hrp_nM": [0.011363636363636364, 0.11363636363636363], "preparation_id": "prep-s8-diluted", "concentration_assigned_by": "dilution_of_given_stock", "dilution_chosen_by": "dilution_search", "aliquot_dilution_factor": 14.0, "aliquot_stock_ul": 20.0, "aliquot_buffer_ul": 260.0, "aliquot_well": "H12", "adopted_well_is_new_reaction": true, "reference_protocol_id": "protocol-hrp-tmb-initial-rate-curve", "reference_event_id": "know-protocol-hrp-tmb-initial-rate-curve", "first_enzyme_ng_ml": 100.0, "first_enzyme_volume_ul": 15.151515151515152, "adopted_enzyme_ng_ml": 5.0, "adopted_enzyme_volume_ul": 10.606060606060606, "adopted_enzyme_source": "H12", "dilution_prescribed": false}, "discarded": ["saturated first attempt"], "commentary": "The only enzyme concentration given is 30 nM. The first wells were prepared at 100 ng/mL from that stock and saturated. The concentration was too high, so the dilution factor was found by measuring a series. Observations obs-s8-A1-t9, obs-s8-A2-t9, obs-s8-A3-t9, and obs-s8-A4-t9 stayed proportional, and obs-s8-A5-t9 did not. The new wells are new reactions from the 14-fold H12 aliquot, 20 uL of the 30 nM stock plus 260 uL PBS, then PBS and TMB. They are not a transfer of the saturated reaction. 5 ng/mL is the concentration that aliquot and the dispensed volume produce. Reported KM = 100 uM and kcat = Vmax/[E] = 3800 /s at 5 ng/mL.", "analysis_report": {"KM_uM": 100.0, "kcat_per_s": 3800.0, "kcat_calculation": {"equation": "kcat_per_s = Vmax_M_per_s / enzyme_concentration_M", "absorbance_conversion": "Vmax_M_per_s = Vmax_A652_per_min / (epsilon_per_M_cm * path_cm * 60)", "concentration_conversion": "ng/mL = nM * hrp_mw_g_per_mol / 1000", "KM_uM": 100.0, "kcat_per_s": 3800.0, "physical_kcat_per_s": 3800.0, "replicate_delta_uM_per_s": 0.0013936164857032004, "fit_KM_uM": 99.99999999999996, "fit_Vmax_uM_per_s": 0.43181818181818177, "fit_r2": 0.9997789628112558, "Vmax_M_per_s": 4.318181818181818e-07, "Vmax_uM_per_s": 0.4318181818181818, "Vmax_A652_per_min": 0.5658545454545455, "epsilon_per_M_cm": 39000.0, "path_cm": 0.56, "wavelength_nm": 652, "hrp_mw_g_per_mol": 44000.0, "enzyme_stock_nM": 30.0, "enzyme_stock_volume_ul": 2000.0, "enzyme_stock_ng_ml": 1320.0, "enzyme_volume_ul": 10.606060606060606, "neat_enzyme_ul": 0.7575757575757576, "enzyme_source_nM": 2.142857142857143, "enzyme_source_well": "H12", "enzyme_source_labware": "plate", "dilution_factor": 14, "total_volume_ul": 200.0, "enzyme_concentration_M": 1.1363636363636364e-10, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "physical_enzyme_concentration_M": 1.1363636363636364e-10, "physical_enzyme_concentration_nM": 0.11363636363636363, "physical_enzyme_concentration_ng_ml": 5.0, "kcat_basis": "prepared_well", "kcat_basis_note": "Reported kcat divides Vmax by the enzyme concentration in the new well. The given stock is 30 nM. H12 is a 14-fold aliquot, 20 uL of that stock plus 260 uL PBS. The adopted well is a new reaction: that aliquot, then PBS and TMB. It is not a transfer of the saturated 100 ng/mL preparation. 5 ng/mL is the concentration those volumes produce, and that is the concentration in the denominator.", "enzyme_stock_nM_physical": 30.0, "enzyme_stock_ng_ml_physical": 1320.0, "concentration_basis": "The only enzyme concentration given is 30 nM. The first wells were 100 ng/mL and saturated. The dilution factor was found by measuring a series. Reported kcat uses 5 ng/mL, the concentration of the new well prepared from the 14-fold H12 aliquot."}, "working_range": {"analyte": "HRP", "criterion": "initial rate proportional to final HRP concentration", "fixed_substrate_uM": 200.0, "linear_ng_ml": [0.5, 5.0], "linear_nM": [0.011363636363636364, 0.11363636363636363], "assay_enzyme_ng_ml": 5.0, "assay_enzyme_nM": 0.11363636363636363, "points": [{"hrp_ng_ml": 0.5, "hrp_nM": 0.011363636363636364, "enzyme_volume_ul": 1.0606060606060606, "neat_enzyme_ul": 0.07575757575757576, "hrp_source": "H12", "hrp_source_labware": "plate", "enzyme_source_nM": 2.142857142857143, "dilution_factor": 14, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.03772363636363637, "rate_uM_per_s": 0.028787878787878782, "in_linear_range": true}, {"hrp_ng_ml": 1.0, "hrp_nM": 0.022727272727272728, "enzyme_volume_ul": 2.121212121212121, "neat_enzyme_ul": 0.15151515151515152, "hrp_source": "H12", "hrp_source_labware": "plate", "enzyme_source_nM": 2.142857142857143, "dilution_factor": 14, "tmb_volume_ul": 8.0, "buffer_volume_ul": 189.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.07544727272727274, "rate_uM_per_s": 0.057575757575757565, "in_linear_range": true}, {"hrp_ng_ml": 2.0, "hrp_nM": 0.045454545454545456, "enzyme_volume_ul": 4.242424242424242, "neat_enzyme_ul": 0.30303030303030304, "hrp_source": "H12", "hrp_source_labware": "plate", "enzyme_source_nM": 2.142857142857143, "dilution_factor": 14, "tmb_volume_ul": 8.0, "buffer_volume_ul": 187.75757575757575, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.15089454545454548, "rate_uM_per_s": 0.11515151515151513, "in_linear_range": true}, {"hrp_ng_ml": 5.0, "hrp_nM": 0.11363636363636363, "enzyme_volume_ul": 10.606060606060606, "neat_enzyme_ul": 0.7575757575757576, "hrp_source": "H12", "hrp_source_labware": "plate", "enzyme_source_nM": 2.142857142857143, "dilution_factor": 14, "tmb_volume_ul": 8.0, "buffer_volume_ul": 181.3939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.3772363636363637, "rate_uM_per_s": 0.2878787878787879, "in_linear_range": true}, {"hrp_ng_ml": 10.0, "hrp_nM": 0.22727272727272727, "enzyme_volume_ul": 1.5151515151515151, "neat_enzyme_ul": 1.5151515151515151, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 30.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.712557575757576, "rate_uM_per_s": 0.5437710437710438, "in_linear_range": false}, {"hrp_ng_ml": 20.0, "hrp_nM": 0.45454545454545453, "enzyme_volume_ul": 3.0303030303030303, "neat_enzyme_ul": 3.0303030303030303, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 30.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 1.2826036363636366, "rate_uM_per_s": 0.9787878787878789, "in_linear_range": false}, {"hrp_ng_ml": 40.0, "hrp_nM": 0.9090909090909091, "enzyme_volume_ul": 6.0606060606060606, "neat_enzyme_ul": 6.0606060606060606, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 30.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 185.93939393939394, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 2.1376727272727276, "rate_uM_per_s": 1.6313131313131313, "in_linear_range": false}, {"hrp_ng_ml": 80.0, "hrp_nM": 1.8181818181818181, "enzyme_volume_ul": 12.121212121212121, "neat_enzyme_ul": 12.121212121212121, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 30.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 179.87878787878788, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 3.2065090909090914, "rate_uM_per_s": 2.446969696969697, "in_linear_range": false}], "linear_ng_ml_basis": "dilution_search"}}}