class P(Protocol):
    x: int

class C:
    def initialize(self) -> None:
        self.x = 0

c = C()
isinstance(c, P)  # False
c.initialize()
isinstance(c, P)  # True

def f(x: Union[P, int]) -> None:
    if isinstance(x, P):
        # Static type of x is P here.
        ...
    else:
        # Static type of x is int, but can be other type at runtime...
        print(x + 1)

f(C())  # ...causing a TypeError.