diff --git a/.gitattributes b/.gitattributes index 40da397d2510fc4b3b8cf7a0db5a0f147c418a5e..05f83e26e16790c59133cc5e0fea3c36026197c1 100644 --- a/.gitattributes +++ b/.gitattributes @@ -87,3 +87,10 @@ wu_20261105_drive_recurrence/manuscript.pdf filter=lfs diff=lfs merge=lfs -text wu_20261106_temporal_robustness/manuscript.pdf filter=lfs diff=lfs merge=lfs -text wu_20261107_temporal_replicates_20261107/manuscript.pdf filter=lfs diff=lfs merge=lfs -text wu_20261106_evidence_synthesis/manuscript.pdf filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/briefing.pdf filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/briefing.pptx filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/figures/ExtendedData5.pdf filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/figures/ExtendedData6.pdf filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/figures/ExtendedData7.pdf filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/figures/Figure1.pdf filter=lfs diff=lfs merge=lfs -text +wu_20261107_paper_figures/manuscript.pdf filter=lfs diff=lfs merge=lfs -text diff --git a/wu_20261107_paper_figures/METHODS_RESULTS_zh.md b/wu_20261107_paper_figures/METHODS_RESULTS_zh.md new file mode 100644 index 0000000000000000000000000000000000000000..5ed24fb56e6a167d4afe405330a9c60bb7973736 --- /dev/null +++ b/wu_20261107_paper_figures/METHODS_RESULTS_zh.md @@ -0,0 +1,17 @@ +# 当前方法与结论：结构—循环—时序—生理 + +我们现在回答的是：同一真实结构的网络，其输入整合如何受循环增益和时间组织共同塑造。文章按研究论文组织，围绕这个问题呈现结果，不再按任务轮次堆叠进度。 + +方法保留5,177个KC的胞体/骨架、突触位置、方向及伙伴身份，结合条件空模型和两脑结构预测；网络使用原32个KC、两个真实MBON14、1,926个循环接点和144个输出接点。外部和循环电导独立控制，时序分析固定接点、权重及每接点18次事件；以操作性波形、平均电压、潜伏期和连续轨迹表征输出。公开生理比较以Fly-ID作评价单位，训练来源和留出来源分开。 + +最核心的结果是： + +1. 在固定外部输入1 nS时，循环电导从0.1升到1 nS，使A条件响应由1个KC/18次波形扩大到32个KC/559次波形。 +2. 相同事件预算下，三种抖动时序的ABC减逐细胞最强单输入计数差依次为+27、+29、−29。时间安排改变了混合比较的方向；成簇时序还使V/L由静默变为响应。 +3. 每一时序内部，32个细胞共享一个二值支持集；连续轨迹仍有差异，第一共享模式占约95.5%能量。 +4. 结构联合特征的表面预测增益主要被区域/分支条件统计复现；两脑预测未表现出稳定联合优势。 +5. 公开生理全部作者QC判定重建一致。固定模型/经验基线全7留出误差为35.216/32.723 mV，通过QC的4留出为15.820/14.192 mV。 + +这些结果把结构分类、响应范围和生理迁移拆解成可以直接检验的问题。原32/15/14及未解释14保留；模型条件和细胞不充当独立动物样本。下一步聚焦跨接点独立异步输入、时序配对细化，以及标签与电流校准明确的公开确认数据。 + +本轮交付21页英文稿、五张复合主图/21子图、十张扩展图、10页中文汇报、原创脑与回路SVG、全部紧凑源表、图代码及已独立编译的Overleaf包。论文没有以“半成品实验”的方式组织；对证据范围的必要限定集中在方法和对应图注。本轮为论文与图版重组，不新增模拟、动物或湿实验。 diff --git a/wu_20261107_paper_figures/README.md b/wu_20261107_paper_figures/README.md new file mode 100644 index 0000000000000000000000000000000000000000..6432a9dae533af13979da51fff06611910169de7 --- /dev/null +++ b/wu_20261107_paper_figures/README.md @@ -0,0 +1,33 @@ +# Structure-to-dynamics article and vector figures — 7 November 2026 + +The article follows one physical substrate from structural representation to recurrent recruitment, timing-dependent mixture responses, continuous heterogeneity and public physiological transfer. It reuses sealed scientific outputs and introduces a complete paper, five new composite main figures (21 panels), source data, original editable brain/circuit illustrations and an accompanying ten-slide briefing. + +- [Article PDF](exports/manuscript.pdf), [English source](manuscript/article.md), [LaTeX](manuscript/main.tex), [separate bibliography](manuscript/references.bib), [Overleaf ZIP](exports/overleaf_submission.zip). +- [Chinese PPT](exports/吴老师汇报_结构与时序整合_20261107.pptx), [PDF](exports/吴老师汇报_结构与时序整合_20261107.pdf). +- [Original brain SVG](assets/mushroom_body.svg), [circuit SVG](assets/synapse_circuit.svg), [five main SVG/PDF figures](figures/). +- [Current findings](当前方法与结论.md), [source tables](source_data/), [provenance](metadata/figure_provenance.json), [checks](validation/), [publication receipt](metadata/publication_receipt.json). + +## Paper and figure design + +Figure 1 links conceptual brain location, measured skeletons/endpoints and the structural benchmark. Figure 2 separates external drive from recurrence. Figure 3 places the fixed-event timing design beside population maps and signed within-cell mixture contrasts. Figure 4 connects the common mode to continuous differences and observed numerical changes. Figure 5 combines physiological design, paired Fly-ID errors and author-QC reconstruction. + +The five main figures are original SVG compositions, not matplotlib exports or flattened screenshots. Every main SVG contains editable text, paths and shapes; there are no embedded raster images. All 32 measured skeletons are included. Concept diagrams are explicitly labelled schematic; measured XZ geometry has its own physical scale. The two original illustrative SVG assets and patch-clamp drawing were authored for this project. No Nature/BioRender illustration was copied. The style-study sources and borrowing limits are documented in `references/DESIGN_SOURCES.md`. + +The manuscript uses a discovery-led abstract, a continuous Results argument, a compact Discussion, detailed Methods, nine verified references and ten retained Extended Data figures. Necessary scope and numerical qualifications appear at the relevant result and in Methods rather than as repeated status disclaimers. The manuscript does not infer authorship, affiliations, subtype physiology, natural stimulus validation or new biological replicates. + +## Reproduce + +Use Python 3.11 with the recorded package versions; LaTeX/pdfLaTeX + BibTeX and LibreOffice are used for paper and slide rendering. From the workspace root: + +```bash +.venv_fine_structure/bin/python projects/wu_20261107_paper_figures/code/build_figures.py +.venv_fine_structure/bin/python projects/wu_20261107_paper_figures/code/build_paper.py +.venv_fine_structure/bin/python projects/wu_20261107_paper_figures/code/build_slides.py +.venv_fine_structure/bin/python projects/wu_20261107_paper_figures/code/verify_release.py +``` + +`build_figures.py` needs only this project's compact source data and original assets. `build_paper.py` uses the included Extended Data PDFs and verified reference metadata if the historical workspace is absent. The Overleaf ZIP is independently extracted and compiled, with page text checked against the working PDF. `prepare_inputs.py` is the optional original-workspace retrieval step, not a required replay step. This paper revision adds no simulations or animals. The historical correction remains in `wu_20261106_evidence_synthesis/CORRECTION_LEDGER.md` and the linked immutable source release. + +## Data and code update + +Code is published on `ydchen0806/bio_fly_cyber_lateralization`, branch `research/wu-structure-function-20261104`. Data, all SVG/PDF artwork, manuscript, slides and the compiling Overleaf ZIP use the additive dataset prefix `wu_20261107_paper_figures` in `cyd0806/neuronmimic-data`. Publication commits and remote identity/hash checks are recorded in the receipt. Prior source and simulation objects retain their immutable revisions and original third-party terms; no blanket third-party licence is assigned. diff --git a/wu_20261107_paper_figures/archive_members.json b/wu_20261107_paper_figures/archive_members.json new file mode 100644 index 0000000000000000000000000000000000000000..5648af77a810b8281517f0595cbe0cce94939123 --- /dev/null +++ b/wu_20261107_paper_figures/archive_members.json @@ -0,0 +1,647 @@ +[ + { + "path": "projects/wu_20261107_paper_figures/README.md", + "bytes": 4354, + "sha256": "b250b08be1f8e19991d914956e84a4896e2212cfde58ccfb9a0f4314fc681c9c" + }, + { + "path": "projects/wu_20261107_paper_figures/REPRODUCIBILITY.md", + "bytes": 1812, + "sha256": "6389956724d412416aa3d41f93bee9e6457c76b93193c6fd947b4a7964ff2974" + }, + { + "path": "projects/wu_20261107_paper_figures/assets/mushroom_body.pdf", + "bytes": 5911, + "sha256": "5d40f0996b32ecacab262e4b3d4b4922b924b6ceea540d32725d1aaa2094cda8" + }, + { + "path": 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Regions locate the computation conceptually; quantitative anatomy is displayed separately from measured skeleton coordinates. + + + + + + + + + + + + + + + + + + + + + + Drosophila brain + Calyx + Peduncle + Lobes + Mushroom body + Conceptual schematic · not to scale + + diff --git a/wu_20261107_paper_figures/assets/synapse_circuit.pdf b/wu_20261107_paper_figures/assets/synapse_circuit.pdf new file mode 100644 index 0000000000000000000000000000000000000000..76e83fe3e2f663c23f8d29d241a6410b02128ba3 Binary files /dev/null and b/wu_20261107_paper_figures/assets/synapse_circuit.pdf differ diff --git a/wu_20261107_paper_figures/assets/synapse_circuit.svg b/wu_20261107_paper_figures/assets/synapse_circuit.svg new file mode 100644 index 0000000000000000000000000000000000000000..b72c8cc154bf9948a3bd641837472f176c6684b0 --- /dev/null +++ b/wu_20261107_paper_figures/assets/synapse_circuit.svg @@ -0,0 +1,16 @@ + + Contact-resolved drive and recurrenceOriginal editable circuit schematic. Positions and edge widths are illustrative. 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Model readouts and descriptive endpoints use frozen source data; conceptual illustrations are labelled schematic. + + + Anatomical structure anchors a synapse-resolved model + + a + Mushroom-body context + + Drosophila brain and bilateral mushroom-body circuit + Original editable vector schematic, frontal conceptual view, not atlas registered and not to scale. Regions locate the computation conceptually; quantitative anatomy is displayed separately from measured skeleton coordinates. + + + + + + + + + + + + + + + + + + + + + + Drosophila brain + Calyx + Peduncle + Lobes + Mushroom body + Conceptual schematic · not to scale + + + 5,177 KCs → frozen 61-cell cohort → 32-cell network + + + b + Real skeletons and measured synapses + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 50 µm + XZ projection · original cell order + Teal: first eligible KC; gold/red: input/output endpoints + + + c + Identity, direction and position in one representation + + + + + Partner A + Partner V + Output + XYZ position + Pre/post direction + Partner identity + Branch context + Conceptual encoding; all source endpoints retained + + + d + Conditioned structure accounts for joint-feature gain + + + + .052 + + .055 + + .058 + Squared Hellinger error ↓ + Anatomy + + 0.05807 + Separate + + 0.05384 + Joint + + 0.05219 + Region null + + 0.05221 + Branch null + + 0.05219 + 5,177 KCs; 18 parent classes; 19 null draws per family + + diff --git a/wu_20261107_paper_figures/figures/Figure2.pdf b/wu_20261107_paper_figures/figures/Figure2.pdf new file mode 100644 index 0000000000000000000000000000000000000000..02497c704e533bb3187d955101c0bbdfeac5cd9f Binary files /dev/null and b/wu_20261107_paper_figures/figures/Figure2.pdf differ diff --git a/wu_20261107_paper_figures/figures/Figure2.svg b/wu_20261107_paper_figures/figures/Figure2.svg new file mode 100644 index 0000000000000000000000000000000000000000..3be01a02e0af8b251e8b16a8be165a6e4be0b751 --- /dev/null +++ b/wu_20261107_paper_figures/figures/Figure2.svg @@ -0,0 +1,197 @@ + + + Recurrent gain recruits a distributed network response + Original editable vector composition. Model readouts and descriptive endpoints use frozen source data; conceptual illustrations are labelled schematic. + + + Recurrent gain recruits a distributed network response + + a + Contact-resolved circuit and interventions + + Contact-resolved drive and recurrenceOriginal editable circuit schematic. Positions and edge widths are illustrative. Counts refer to the frozen 32-KC and two-MBON model. + + + A · ALPNV · direct VPNL · candidate + + + + + + + MBON + Real KC cable treesE: boundary driveR: recurrence + 32 KCs · 1,926 recurrent contacts · 144 output contacts + + + + + b + Eligibility follows actual input contacts + A + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + V + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + L + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 1 + 32 + Frozen KC index + 10 directly driven · 22 connected without boundary drive + Darker: more contacts (log scale); teal: three-family eligibility + + + c + External drive and recurrence define distinct regimes + + 0 + 0 / 32 KCs + + 0 + 0 / 32 KCs + + 18 + 1 / 32 KCs + + 559 + 32 / 32 KCs + 0.1 + 1.0 + 0.1 + 1.0 + Recurrent conductance R (nS) + External E (nS) + A-only operational waveform counts; fixed real contacts + + + d + At fixed drive, recurrence expands recruitment + + + + R = 0.1 + + R = 1 + + 0 + + 200 + + 400 + + 600 + Recurrence at E = 1 nS + KC waveform count + + + 18 + 1 KC + + 559 + 32 KCs + Main mesh comparison; weak-E primary comparison is refined + + diff --git a/wu_20261107_paper_figures/figures/Figure3.pdf b/wu_20261107_paper_figures/figures/Figure3.pdf new file mode 100644 index 0000000000000000000000000000000000000000..4c78fae0ff51849b8bace08d77e02b58cb8e4bf1 Binary files /dev/null and b/wu_20261107_paper_figures/figures/Figure3.pdf differ diff --git a/wu_20261107_paper_figures/figures/Figure3.svg b/wu_20261107_paper_figures/figures/Figure3.svg new file mode 100644 index 0000000000000000000000000000000000000000..4cf271d66fbd71c19e21dcbe0b99d541dbbef2a3 --- /dev/null +++ b/wu_20261107_paper_figures/figures/Figure3.svg @@ -0,0 +1,428 @@ + + + Event timing changes the sign of the mixture contrast + Original editable vector composition. Model readouts and descriptive endpoints use frozen source data; conceptual illustrations are labelled schematic. + + + Event timing changes the sign of the mixture contrast + + a + Event budgets fixed; only times vary + Sync + + + + + + + + + + + + + + + + + + + Jitter 06 + + + + + + + + + + + + + + + + + + + Jitter 07 + + + + + + + + + + + + + + + + + + + Jitter 08 + + + + + + + + + + + + + + + + + + + Bursty + + + + + + + + + + + + + + + + + + + + 100 + 950 ms + 18 events/contact · E = R = 1 nS + + + b + Input-output maps depend on timing + A + V + L + ABC + Sync + + 559 + + 0 + + 0 + + 559 + Jitter 06 + + 524 + + 0 + + 0 + + 551 + Jitter 07 + + 406 + + 0 + + 0 + + 435 + Jitter 08 + + 494 + + 0 + + 0 + + 465 + Bursty + + 96 + + 96 + + 96 + + 96 + Operational waveform count, summed over 32 KCs + + + c + Within-cell mixture contrasts reverse direction + + + + Sync + + Jitter 06 + + Jitter 07 + + Jitter 08 + + Bursty + + -4 + + -2 + + 0 + + 2 + + 4 + ABC - best single (count) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Σ +0 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Σ +27 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Σ +29 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Σ -29 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Σ +0 + Points: original 32 cells, deterministically offset to expose ties + + + d + Voltage contrasts tell the same directional story + + + + Sync + + Jitter 06 + + Jitter 07 + + Jitter 08 + + Bursty + + -2 + + 0 + + 4 + + 8 + + 12 + Mean voltage contrast (mV) + + + + + + + + + + + + + + + + + + + + + + + ABC - mean singles + + ABC - best single + Same sealed runs; two distinct reference contrasts + + + e + Shared response support across all 32 KCs + Sync + A / ABC + Jitter 06 + A / ABC + Jitter 07 + A / ABC + Jitter 08 + A / ABC + Bursty + A / V / L / ABC + One support set per schedule + 32 cells in every tested set + + diff --git a/wu_20261107_paper_figures/figures/Figure4.pdf b/wu_20261107_paper_figures/figures/Figure4.pdf new file mode 100644 index 0000000000000000000000000000000000000000..84b8bcb38f860775b86ef8559191e970105e0030 Binary files /dev/null and b/wu_20261107_paper_figures/figures/Figure4.pdf differ diff --git a/wu_20261107_paper_figures/figures/Figure4.svg b/wu_20261107_paper_figures/figures/Figure4.svg new file mode 100644 index 0000000000000000000000000000000000000000..babf0d40237b669f0349b33feed6afa11195c3b8 --- /dev/null +++ b/wu_20261107_paper_figures/figures/Figure4.svg @@ -0,0 +1,1269 @@ + + + Continuous heterogeneity lies within a dominant common response + Original editable vector composition. Model readouts and descriptive endpoints use frozen source data; conceptual illustrations are labelled schematic. + + + Continuous heterogeneity lies within a dominant common response + + a + Profile amplitudes resolve across the full cohort + + + + 1 + + 16 + + 32 + + 0.0 + + 3.5 + + 7.0 + + 10.5 + + 14.0 + Frozen KC index + Profile RMS (mV) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Teal: directly driven; blue: indirect; red: refinement RMS + + + b + A shared mode carries most profile energy + + + + 1 + + 2 + + 3 + + 4 + + 5 + + 6 + + 0 + + 0.5 + + 1 + Singular mode + Fraction of profile energy + + + + + + + 95.5% + first common mode + + + c + Pairwise differences versus refinement changes + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 1 + 32 + Frozen KC index + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 1 + 10 + 100 + 1000 + Distance / discrepancy + + + d + Pair counts across diagnostic thresholds + + + + 1 + + 3 + + 5 + + 10 + + 0 + + 250 + + 496 + Distance / discrepancy threshold + Cell pairs above threshold + + + 493 + + 395 + + 260 + + 61 + 496 pairs; descriptive numerical comparisons + + diff --git a/wu_20261107_paper_figures/figures/Figure5.pdf b/wu_20261107_paper_figures/figures/Figure5.pdf new file mode 100644 index 0000000000000000000000000000000000000000..61018c1bb5020259feef9548988c6093e96732fe Binary files /dev/null and b/wu_20261107_paper_figures/figures/Figure5.pdf differ diff --git a/wu_20261107_paper_figures/figures/Figure5.svg b/wu_20261107_paper_figures/figures/Figure5.svg new file mode 100644 index 0000000000000000000000000000000000000000..e686197e67196a83c51518c5d61a11f9eaec160c --- /dev/null +++ b/wu_20261107_paper_figures/figures/Figure5.svg @@ -0,0 +1,160 @@ + + + Public physiology tests the transfer of a fixed model + Original editable vector composition. Model readouts and descriptive endpoints use frozen source data; conceptual illustrations are labelled schematic. + + + Public physiology tests the transfer of a fixed model + + a + Published recordings with animal-level holdout + + + + Fly IDs 8–10 + Published parameters + waveform mean + + Fly IDs 1–7 + 40 / 80 / 120 nominal pA + Fixed models; no holdout refitting + 24 ABFs · 480 sweeps · source subtype labels unspecified + + + b + Fixed model versus training-waveform baseline + + + + Fixed WT9 + + Empirical mean + + 0 + + 25 + + 50 + + 75 + Per-Fly-ID trace RMSE (mV) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Pairs: seven Fly IDs; circles: source-QC passed + + + c + Source-quality decisions are reconstructed exactly + + + + 0 + + 1 + + 2 + + 3 + + 4 + + 0 + + 1 + + 2 + + 3 + + 4 + Source input resistance (GΩ) + Reconstructed resistance (GΩ) + + + + + + + + + + + + + + + + + + + + + + + + + + + + 24 / 24 inclusion decisions; dashed line: 0.8 GΩ + + + d + Source QC 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No explicit file ID in workbook.,0.414825561520021,0.41482535941488274,4.872051218018925e-07,7.900715515642889,1.259379958287453,True,True,-20.1416015625,-40,231.26 +WT,2,75,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,3.09802985984673,3.098028894748135,3.115201075987173e-07,46.207768791720156,13.60266054191366,False,False,-20.1416015625,-40,231.26 +WT,3,76,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.268968531319347,0.2689685252618688,2.2521140841114118e-08,3.213517802541524,0.3100224784305743,True,True,-20.1416015625,-40,231.26 +WT,4,77,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.0259887340761,1.0259911969025521,2.4004420031698004e-06,6.289053538772674,34.640841427753294,False,False,-19.83642578125,-40,231.26 +WT,5,78,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.63283385466921,1.632833808877508,2.8044311983776993e-08,28.32576134384206,4.981681104337597,False,False,-20.1416015625,-40,231.26 +WT,6,79,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.80336235952451,0.8033623392397684,2.5249803248250657e-08,16.033757517569413,4.177336399306763,False,False,-20.1416015625,-40,231.26 +WT,7,80,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.436521744129391,0.43652173450416365,2.204982330056282e-08,5.722568494916409,0.6458197578326936,True,True,-20.1416015625,-40,231.26 +WT,8,81,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.28981251120351,1.2898124876391266,1.8269619150856897e-08,18.434534193154697,1.7331733559718976,False,False,-20.1416015625,-40,231.26 +WT,9,82,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.09934918692818,1.0993485032939996,6.218535371083078e-07,14.65783479839107,4.964906256413454,False,False,-20.1416015625,-40,231.26 +WT,10,83,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.35560245083886,1.3556024197571355,2.292834788697462e-08,23.267138587080517,1.0877538583497626,False,False,-20.1416015625,-40,231.26 +NaChBac,1,29,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.295389783740758,0.29538977073224937,4.403845144107521e-08,3.623043616611295,0.2073128786959268,True,True,-20.1416015625,-40,231.26 +NaChBac,2,30,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.646970062133017,0.646969927249873,2.0848436721454533e-07,7.767632893435204,5.983315571916739,True,True,-19.83642578125,-40,231.26 +NaChBac,3,31,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,2.16287206000063,2.162871999031595,2.8188923306693425e-08,28.8313254823468,2.1991206870772273,False,False,-20.1416015625,-40,231.26 +NaChBac,4,32,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.592717337718566,0.5927147249384301,4.408138533529282e-06,2.09410079649728,15.195931286839668,True,True,-20.1416015625,-40,231.26 +NaChBac,5,33,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.607413540989338,0.6074135816078371,6.687124409765488e-08,4.906498798713186,1.4550680887070575,True,True,-20.1416015625,-40,231.26 +NaChBac,6,34,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.226205266525797,0.22620523164887207,1.5418263887845285e-07,5.742920581759393,0.33232897894425323,True,True,-20.1416015625,-40,231.26 +NaChBac,7,35,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.593269960894926,0.5932699489915764,2.0063968264594223e-08,6.5039379380659295,0.8114237884876729,True,True,-20.1416015625,-40,231.26 +NaChBac,8,36,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.57645888268982,1.5764607564447348,1.1885847042147901e-06,17.540646166755028,7.817592029631036,False,False,-20.1416015625,-40,231.26 +NaChBac,9,37,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.92168431186752,1.9216841799585669,6.864236345993646e-08,34.98461122278199,2.7471828233890183,False,False,-20.1416015625,-40,231.26 +NaChBac,10,38,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,2.3743599817375,2.3743599661979724,6.5447226527126645e-09,33.32560122911819,2.109211900740331,False,False,-20.1416015625,-40,231.26 +NaChBac,11,39,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,2.06771207512855,2.067713580967968,7.282635896554979e-07,44.83478565940745,5.533784334615831,False,False,-20.1416015625,-40,231.26 +NaChBac,12,40,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.270572480095479,0.270572454557122,9.438638037817526e-08,2.759709119477294,0.5649965496353587,True,True,-20.1416015625,-40,231.26 +NaChBac,13,41,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,0.991253363074116,0.9912533137279451,4.97815924038747e-08,18.107870601590754,1.014371716849287,False,False,-20.1416015625,-40,231.26 +NaChBac,14,42,Condition plus within-condition source row order; checked against full fitted R fingerprint. No explicit file ID in workbook.,1.50964716524111,1.5096471285095803,2.433120183109594e-08,25.724114935615177,1.9155390319322756,False,False,-20.1416015625,-40,231.26 diff --git a/wu_20261107_paper_figures/source_data/cell_contrasts.csv b/wu_20261107_paper_figures/source_data/cell_contrasts.csv new file mode 100644 index 0000000000000000000000000000000000000000..17ec4921cc954137f523bf2f0c463e747fefe728 --- /dev/null +++ b/wu_20261107_paper_figures/source_data/cell_contrasts.csv @@ -0,0 +1,161 @@ +schedule,gid,root_id,eligible,voltage_ABC_minus_mean_mV,voltage_ABC_minus_best_mV,count_ABC_minus_mean,count_ABC_minus_best,responsive_masks +synchronous,0,720575940606579465,False,10.722717279448439,-0.1453703698292088,12.0,0,"1,7" +synchronous,1,720575940606698697,False,13.882245261623417,-0.021404785244165225,12.0,0,"1,7" +synchronous,2,720575940615832577,True,10.31590406087674,0.27390762992723694,12.0,0,"1,7" 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+synchronous,23,720575940630775891,False,12.066500886571148,0.027547055608984294,12.0,0,"1,7" +synchronous,24,720575940630923693,False,10.596623049432445,-0.02531020603552747,12.0,0,"1,7" +synchronous,25,720575940632437331,True,11.22711244600216,0.05388668757680648,12.0,0,"1,7" +synchronous,26,720575940633103059,False,13.626740255681362,-0.23362103488370423,12.0,0,"1,7" +synchronous,27,720575940634514533,False,12.012369596860289,-0.06271642628766472,12.0,0,"1,7" +synchronous,28,720575940634664674,False,12.322190677386864,-0.010887462276116366,12.0,0,"1,7" +synchronous,29,720575940643769544,False,14.55919373006109,-0.10080694333964857,12.0,0,"1,7" +synchronous,30,720575940644413732,False,11.170262990969277,-0.004167616132846774,12.0,0,"1,7" +synchronous,31,720575940649022841,True,10.859833395029632,1.7554775206324749,12.0,0,"1,7" +jittered_seed20261106,0,720575940606579465,False,10.813336340278362,0.6174731718837805,12.333333333333332,1,"1,7" +jittered_seed20261106,1,720575940606698697,False,14.083324742356737,0.9965868513261,12.333333333333332,1,"1,7" +jittered_seed20261106,2,720575940615832577,True,10.67083201944478,0.864075919136404,9.333333333333332,-2,"1,7" +jittered_seed20261106,3,720575940618341952,True,10.875144590761039,0.28338314133046083,4.0,0,"1,7" +jittered_seed20261106,4,720575940618662939,False,11.700818881626112,0.8889023164569849,12.333333333333332,1,"1,7" +jittered_seed20261106,5,720575940619077217,False,11.82417081698248,0.552825197527234,12.333333333333332,1,"1,7" +jittered_seed20261106,6,720575940619897734,False,11.532297653414894,0.7333122194083401,12.333333333333332,1,"1,7" +jittered_seed20261106,7,720575940620333958,False,13.525698368089465,0.9642458066188055,12.333333333333332,1,"1,7" +jittered_seed20261106,8,720575940621606755,False,10.772045150526765,0.8833528865789262,12.333333333333332,1,"1,7" +jittered_seed20261106,9,720575940621770010,True,12.201153815752583,0.8201776814892625,12.333333333333332,1,"1,7" +jittered_seed20261106,10,720575940622003316,False,11.495083714416829,0.7617928832171046,12.333333333333332,1,"1,7" +jittered_seed20261106,11,720575940622412838,False,14.376437942319697,0.8792116303587818,12.333333333333332,1,"1,7" +jittered_seed20261106,12,720575940622836455,True,11.099566299893034,0.8481546994579077,12.333333333333332,1,"1,7" +jittered_seed20261106,13,720575940623973961,False,11.923098444922658,0.674492519939399,12.333333333333332,1,"1,7" +jittered_seed20261106,14,720575940624107755,False,13.37347465880341,0.9834394330603189,11.333333333333332,0,"1,7" +jittered_seed20261106,15,720575940625328915,False,12.953293968832114,0.861401917951234,12.333333333333332,1,"1,7" +jittered_seed20261106,16,720575940625500304,False,12.013782002019575,0.7749458719903615,12.333333333333332,1,"1,7" +jittered_seed20261106,17,720575940626305028,True,12.335903162407888,0.817165188273556,12.333333333333332,1,"1,7" +jittered_seed20261106,18,720575940627585028,False,11.310714240784364,0.8665807983656038,12.333333333333332,1,"1,7" +jittered_seed20261106,19,720575940627626244,False,11.250145583807857,0.6239764658798137,11.333333333333332,0,"1,7" +jittered_seed20261106,20,720575940629319106,True,9.8394175876414,0.4427550323078435,12.333333333333332,1,"1,7" +jittered_seed20261106,21,720575940629507706,True,12.268120813212455,0.0743933597530031,10.0,2,"1,7" +jittered_seed20261106,22,720575940630331244,True,10.373534606733358,0.8660114849142957,12.333333333333332,1,"1,7" +jittered_seed20261106,23,720575940630775891,False,11.59991066829891,0.864227678200649,12.333333333333332,1,"1,7" +jittered_seed20261106,24,720575940630923693,False,10.702627379525914,0.8503534009714784,12.333333333333332,1,"1,7" 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"event_schedule": "jittered_seed20261107", + "event_times_ms": [ + 108.566235, + 155.635523, + 189.400147, + 262.216242, + 289.395773, + 335.533899, + 412.632124, + 463.302699, + 486.575699, + 550.728444, + 612.22194, + 661.279134, + 711.749659, + 736.623704, + 789.820352, + 861.064425, + 910.437498, + 941.739209 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261108_m1", + "panel": "balanced", + "mask": 1, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261108", + "event_times_ms": [ + 109.352006, + 139.384565, + 197.565837, + 247.040458, + 289.008224, + 359.575976, + 388.944074, + 440.686284, + 488.020106, + 541.187354, + 593.050433, + 653.549393, + 707.528559, + 743.9678, + 801.065344, + 842.113277, + 901.148548, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261108_m2", + "panel": "balanced", + "mask": 2, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261108", + "event_times_ms": [ + 109.352006, + 139.384565, + 197.565837, + 247.040458, + 289.008224, + 359.575976, + 388.944074, + 440.686284, + 488.020106, + 541.187354, + 593.050433, + 653.549393, + 707.528559, + 743.9678, + 801.065344, + 842.113277, + 901.148548, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261108_m4", + "panel": "balanced", + "mask": 4, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261108", + "event_times_ms": [ + 109.352006, + 139.384565, + 197.565837, + 247.040458, + 289.008224, + 359.575976, + 388.944074, + 440.686284, + 488.020106, + 541.187354, + 593.050433, + 653.549393, + 707.528559, + 743.9678, + 801.065344, + 842.113277, + 901.148548, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261108_m7", + "panel": "balanced", + "mask": 7, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261108", + "event_times_ms": [ + 109.352006, + 139.384565, + 197.565837, + 247.040458, + 289.008224, + 359.575976, + 388.944074, + 440.686284, + 488.020106, + 541.187354, + 593.050433, + 653.549393, + 707.528559, + 743.9678, + 801.065344, + 842.113277, + 901.148548, + 950.0 + ], + "n_events": 18 + } + ], + "primary": "Within-mask comparison of jitter seeds20261107 and20261108 against the already sealed synchronous reference from wu_20261106_temporal_robustness. Report all32, eligible10 and indirect22 voltage, waveform and latency changes.", + "secondary": "Describe variation across the two independent deterministic jitter realizations; no biological p values, natural sensory interpretation or clustering.", + "fixed": "Same real trees, contacts, external/recurrent weights, balancing, channels, 18 events, main mesh and duration as temporal project; only jitter realization changes.", + "limitations": "Two deterministic timing realizations are computational robustness probes, not natural stimuli or independent animal replication." +} diff --git a/wu_20261107_paper_figures/source_data/representative_endpoints.csv b/wu_20261107_paper_figures/source_data/representative_endpoints.csv new file mode 100644 index 0000000000000000000000000000000000000000..f465bfb945ec4392186ba6d9c3e05865f1d82608 --- /dev/null +++ b/wu_20261107_paper_figures/source_data/representative_endpoints.csv @@ -0,0 +1,573 @@ 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+jittered_seed20261106,7,551,32,-51.9487440983519 +jittered_seed20261107,1,406,32,-56.14146997407278 +jittered_seed20261107,2,0,0,-69.47332323800387 +jittered_seed20261107,4,0,0,-69.424783566559 +jittered_seed20261107,7,435,32,-55.183354635123095 +jittered_seed20261108,1,494,32,-53.45230047240095 +jittered_seed20261108,2,0,0,-69.4695569787789 +jittered_seed20261108,4,0,0,-69.42110327934355 +jittered_seed20261108,7,465,32,-54.42511696460077 +bursty_three_epochs,1,96,32,-67.22704586167686 +bursty_three_epochs,2,96,32,-67.27364448303024 +bursty_three_epochs,4,96,32,-67.2604111604264 +bursty_three_epochs,7,96,32,-67.20574192974635 diff --git a/wu_20261107_paper_figures/source_data/temporal_protocol.json b/wu_20261107_paper_figures/source_data/temporal_protocol.json new file mode 100644 index 0000000000000000000000000000000000000000..d59bf851353d5723bad9c924018c64479f32a242 --- /dev/null +++ b/wu_20261107_paper_figures/source_data/temporal_protocol.json @@ -0,0 +1,433 @@ +{ + "status": "FROZEN_BEFORE_TEMPORAL_ROBUSTNESS_SIMULATIONS", + "motivation": "Test whether the response and source-family contrast depend on one exact synchronous synthetic pulse train while preserving real contacts and conductance.", + "cohort": "Original32 KC plus2 real MBON14; original32/15/14 unchanged; unresolved14 remains unresolved.", + "schedules": { + "synchronous": [ + 100, + 150, + 200, + 250, + 300, + 350, + 400, + 450, + 500, + 550, + 600, + 650, + 700, + 750, + 800, + 850, + 900, + 950 + ], + "jittered_seed20261106": [ + 100.0, + 153.653208, + 192.389854, + 253.092492, + 308.185424, + 351.392994, + 387.745243, + 456.257355, + 502.941092, + 553.776808, + 600.783402, + 646.56213, + 685.536235, + 751.61214, + 808.819561, + 855.670222, + 894.595529, + 950.0 + ], + "bursty_three_epochs": [ + 100, + 105, + 110, + 115, + 120, + 125, + 300, + 305, + 310, + 315, + 320, + 325, + 600, + 605, + 610, + 615, + 620, + 625 + ] + }, + "new_main": [ + { + "condition": "temporal_synchronous_m1", + "panel": "balanced", + "mask": 1, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "synchronous", + "event_times_ms": [ + 100, + 150, + 200, + 250, + 300, + 350, + 400, + 450, + 500, + 550, + 600, + 650, + 700, + 750, + 800, + 850, + 900, + 950 + ], + "n_events": 18 + }, + { + "condition": "temporal_synchronous_m2", + "panel": "balanced", + "mask": 2, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "synchronous", + "event_times_ms": [ + 100, + 150, + 200, + 250, + 300, + 350, + 400, + 450, + 500, + 550, + 600, + 650, + 700, + 750, + 800, + 850, + 900, + 950 + ], + "n_events": 18 + }, + { + "condition": "temporal_synchronous_m4", + "panel": "balanced", + "mask": 4, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "synchronous", + "event_times_ms": [ + 100, + 150, + 200, + 250, + 300, + 350, + 400, + 450, + 500, + 550, + 600, + 650, + 700, + 750, + 800, + 850, + 900, + 950 + ], + "n_events": 18 + }, + { + "condition": "temporal_synchronous_m7", + "panel": "balanced", + "mask": 7, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "synchronous", + "event_times_ms": [ + 100, + 150, + 200, + 250, + 300, + 350, + 400, + 450, + 500, + 550, + 600, + 650, + 700, + 750, + 800, + 850, + 900, + 950 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261106_m1", + "panel": "balanced", + "mask": 1, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261106", + "event_times_ms": [ + 100.0, + 153.653208, + 192.389854, + 253.092492, + 308.185424, + 351.392994, + 387.745243, + 456.257355, + 502.941092, + 553.776808, + 600.783402, + 646.56213, + 685.536235, + 751.61214, + 808.819561, + 855.670222, + 894.595529, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261106_m2", + "panel": "balanced", + "mask": 2, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261106", + "event_times_ms": [ + 100.0, + 153.653208, + 192.389854, + 253.092492, + 308.185424, + 351.392994, + 387.745243, + 456.257355, + 502.941092, + 553.776808, + 600.783402, + 646.56213, + 685.536235, + 751.61214, + 808.819561, + 855.670222, + 894.595529, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261106_m4", + "panel": "balanced", + "mask": 4, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261106", + "event_times_ms": [ + 100.0, + 153.653208, + 192.389854, + 253.092492, + 308.185424, + 351.392994, + 387.745243, + 456.257355, + 502.941092, + 553.776808, + 600.783402, + 646.56213, + 685.536235, + 751.61214, + 808.819561, + 855.670222, + 894.595529, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_jittered_seed20261106_m7", + "panel": "balanced", + "mask": 7, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "jittered_seed20261106", + "event_times_ms": [ + 100.0, + 153.653208, + 192.389854, + 253.092492, + 308.185424, + 351.392994, + 387.745243, + 456.257355, + 502.941092, + 553.776808, + 600.783402, + 646.56213, + 685.536235, + 751.61214, + 808.819561, + 855.670222, + 894.595529, + 950.0 + ], + "n_events": 18 + }, + { + "condition": "temporal_bursty_three_epochs_m1", + "panel": "balanced", + "mask": 1, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "bursty_three_epochs", + "event_times_ms": [ + 100, + 105, + 110, + 115, + 120, + 125, + 300, + 305, + 310, + 315, + 320, + 325, + 600, + 605, + 610, + 615, + 620, + 625 + ], + "n_events": 18 + }, + { + "condition": "temporal_bursty_three_epochs_m2", + "panel": "balanced", + "mask": 2, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "bursty_three_epochs", + "event_times_ms": [ + 100, + 105, + 110, + 115, + 120, + 125, + 300, + 305, + 310, + 315, + 320, + 325, + 600, + 605, + 610, + 615, + 620, + 625 + ], + "n_events": 18 + }, + { + "condition": "temporal_bursty_three_epochs_m4", + "panel": "balanced", + "mask": 4, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "bursty_three_epochs", + "event_times_ms": [ + 100, + 105, + 110, + 115, + 120, + 125, + 300, + 305, + 310, + 315, + 320, + 325, + 600, + 605, + 610, + 615, + 620, + 625 + ], + "n_events": 18 + }, + { + "condition": "temporal_bursty_three_epochs_m7", + "panel": "balanced", + "mask": 7, + "weight_uS": 0.001, + "external_weight_uS": 0.001, + "recurrent_weight_uS": 0.001, + "event_schedule": "bursty_three_epochs", + "event_times_ms": [ + 100, + 105, + 110, + 115, + 120, + 125, + 300, + 305, + 310, + 315, + 320, + 325, + 600, + 605, + 610, + 615, + 620, + 625 + ], + "n_events": 18 + } + ], + "primary": "At fixed E=R=1 nS, compare each non-synchronous schedule with synchronous within each mask using all32 voltage, waveform count, latency and ABC-minus-mean-single readouts. No natural sensory inference or cell-as-animal p values.", + "secondary": "Describe schedule effects separately for eligible10 and indirect22 and compare source-mask contrasts. No biological functional clustering.", + "fixed": "Same real trees, contacts, ion channels, external balancing, 18 events per active contact, 1200ms duration, main mesh KC0.25um/MBON0.5um/dt0.0025ms. Only event times differ.", + "limitations": "Deterministic synthetic temporal perturbations, not natural stimuli; this is computational robustness, not independent animal or physiology validation." +}