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a/paper-releases/pbisc-diffusion/updates/20261015T141849Z-rare-cell-7k-8k-panels/diffusion/dataset/server_1/model8/plans/rare_gene_context_expansion_20261015/coordination/ilc_training_support/extraction_code_snapshot.py b/paper-releases/pbisc-diffusion/updates/20261015T141849Z-rare-cell-7k-8k-panels/diffusion/dataset/server_1/model8/plans/rare_gene_context_expansion_20261015/coordination/ilc_training_support/extraction_code_snapshot.py new file mode 100644 index 0000000000000000000000000000000000000000..73f5d0f09bcedbb4a2e2bc93a76c04d25977e4ce --- /dev/null +++ b/paper-releases/pbisc-diffusion/updates/20261015T141849Z-rare-cell-7k-8k-panels/diffusion/dataset/server_1/model8/plans/rare_gene_context_expansion_20261015/coordination/ilc_training_support/extraction_code_snapshot.py @@ -0,0 +1,342 @@ +#!/usr/bin/env python3 +"""Bounded, CPU-only source-labelled TRAIN ILC marker support audit. + +Reuses the chunk/row algorithm in corpus_macrophage_markers_20261015. +Selection is fixed by source L1 annotation and registered split, never expression. +""" +from __future__ import annotations + +import argparse +from collections import defaultdict +import hashlib +import json +from pathlib import Path +import resource +import time + +import h5py +import numpy as np +import pandas as pd +import pyarrow.parquet as pq +import zarr + +ROOT = Path(__file__).resolve().parent +PLAN = ROOT.parents[1] +M = PLAN.parents[1] +M7 = M.parent / 'model_7_no_annotation_sh_hwang/data' +E2 = M7 / 'e2_full_source' +BLOCK = Path('/home/sj_server_1/PBISC/PBISC-data/single-cell-data-block-blood') +KEY = ['dataset_id', 'donor_id', 'sample_id'] +SOURCE_CT = Path('/home/sj_server_1/PBISC/paper-release-20260928/manuscript/source_data/corpus/corpus_celltypes.csv') +zarr.config.set({'async.concurrency': 2, 'threading.max_workers': 2}) + + +def sha(path: Path) -> str: + h = hashlib.sha256() + with Path(path).open('rb') as f: + for b in iter(lambda: f.read(1 << 20), b''): h.update(b) + return h.hexdigest() + + +def receipt(path: Path) -> dict: + p = Path(path) + return {'path': str(p), 'bytes': p.stat().st_size, 'sha256': sha(p)} + + +def dump(name: str, value: dict | list) -> None: + (ROOT / name).write_text(json.dumps(value, indent=2) + '\n') + + +def targets() -> pd.DataFrame: + p = ROOT / 'locked_target_genes.csv' + t = pd.read_csv(p) + assert len(t) == 48 and not t.gene_id.duplicated().any() + return t + + +def prepare() -> None: + start = time.time() + target_source = PLAN / 'ilc_targeted_audit_genes.csv' + t = pd.read_csv(target_source) + t.to_csv(ROOT / 'locked_target_genes.csv', index=False) + t = targets() + cols = t.raw_panel_position.to_numpy(dtype=int) + var = pd.read_parquet(E2 / 'metadata/var.parquet') + assert len(var) == 20097 + assert var.iloc[cols].gene_id.tolist() == t.gene_id.tolist() + assert var.iloc[cols].gene_symbol.tolist() == t.gene_symbol.tolist() + assert var.iloc[cols].panel_index.tolist() == cols.tolist() + cfgp = M / 'runs/f01_6k_1x_seed20260627/effective_config.json' + cfg = json.loads(cfgp.read_text())['data'] + assert cfg['n_genes'] == 6000 + manifest_path = Path(cfg['gene_selection_manifest']) + position_path = Path(cfg['gene_positions_path']) + assert sha(manifest_path) == cfg['gene_selection_manifest_sha256'] + assert sha(position_path) == cfg['gene_positions_sha256'] + manifest = json.loads(manifest_path.read_text()) + selected_path = Path(manifest['selected_genes_path']) + assert sha(selected_path) == manifest['selected_genes_sha256'] + selected = pd.read_csv(selected_path) + assert len(selected) == 6000 and not selected.gene_id.duplicated().any() + assert np.array_equal(np.load(position_path), selected.panel_position) + assert var.iloc[selected.panel_position].gene_id.tolist() == selected.gene_id.tolist() + assert np.array_equal(t.in_final6k, t.gene_id.isin(selected.gene_id)) + records = [receipt(p) for p in [target_source, ROOT / 'locked_target_genes.csv', + E2 / 'metadata/var.parquet', SOURCE_CT, cfgp, manifest_path, position_path, selected_path]] + si_path = M7 / 'e2_full_index/metadata/sample_index.parquet' + si = pd.read_parquet(si_path) + for k in KEY: si[k] = si[k].astype(str) + assert not si.duplicated(KEY).any() + records.append(receipt(si_path)) + ct = pd.read_csv(SOURCE_CT) + expected = ct[ct.celltype_l1 == 'ILC'].groupby('dataset_prefix').n_cells.sum().to_dict() + assert sum(expected.values()) == 19329 + files = [p for p in sorted((E2 / 'metadata/obs_sc.parquet').glob('*.parquet')) + if any(p.stem.startswith(k + '_') for k in expected) + and not p.name.endswith('__strict_overlay.parquet')] + selected_rows, checks, all_ilc = [], [], defaultdict(int) + for fi, ep in enumerate(files): + bp = BLOCK / 'metadata/obs_sc.parquet' / ep.name + eq, bq = pq.ParquetFile(ep), pq.ParquetFile(bp) + assert eq.metadata.num_rows == bq.metadata.num_rows + bcols = ['source_row_index', 'row_index', 'celltype_l1'] + extra = [x for x in ['source_celltype_l1', 'label_original', 'source_file'] if x in bq.schema.names] + bi = iter(bq.iter_batches(batch_size=32768, columns=bcols + extra, use_threads=False)) + offset = n_ilc = n_train = 0 + for eb in eq.iter_batches(batch_size=32768, columns=KEY + ['source_row_index', 'row_index'], use_threads=False): + e, b = eb.to_pandas(), next(bi).to_pandas() + assert len(e) == len(b) and np.array_equal(e.source_row_index, b.source_row_index) + assert np.array_equal(e.row_index, np.arange(offset, offset + len(e))) + keep = b.celltype_l1.astype(str).eq('ILC').to_numpy() + if keep.any(): + v = e.loc[keep].copy() + for k in KEY: v[k] = v[k].astype(str) + v['shard_row'] = v.row_index + v['block_pre_qc_row_index'] = b.loc[keep, 'row_index'].to_numpy() + v['source_label'] = b.loc[keep, 'celltype_l1'].astype(str).to_numpy() + for c in extra: v[c] = b.loc[keep, c].astype(str).to_numpy() + v = v.merge(si[KEY + ['split', 'sample_key']], on=KEY, how='left', validate='many_to_one') + assert v.split.notna().all() + prefix = next(k for k in expected if ep.stem.startswith(k + '_')) + all_ilc[prefix] += len(v) + n_ilc += len(v) + v = v[v.split == 'train'].copy() + v['shard_id'] = ep.stem + n_train += len(v) + if len(v): selected_rows.append(v) + offset += len(e) + assert next(bi, None) is None and offset == eq.metadata.num_rows + checks.append({'shard_id': ep.stem, 'metadata_rows': offset, 'source_ilc': n_ilc, + 'train_ilc': n_train, 'source_identity_and_physical_order_match': True}) + records.extend([receipt(ep), receipt(bp)]) + if fi % 20 == 0 or fi + 1 == len(files): print(f'metadata {fi+1}/{len(files)}', flush=True) + assert dict(all_ilc) == expected, (dict(all_ilc), expected) + v = pd.concat(selected_rows, ignore_index=True) + assert len(v) == 16439 and not v.duplicated(['shard_id', 'shard_row']).any() + v.to_parquet(ROOT / 'selected_training_ilc.parquet', index=False) + rows_by_sid = {x['shard_id']: x['metadata_rows'] for x in checks} + plans = [] + for sid, d in v.groupby('shard_id', sort=True): + zp = BLOCK / 'counts/sc_counts' / (sid + '.zarr') + g = zarr.open_group(str(zp), mode='r') + for p in sorted(zp.glob('**/zarr.json')): records.append(receipt(p)) + assert g.attrs['shape'] == [rows_by_sid[sid], 20097] and g.attrs['shard_id'] == sid + mask = np.asarray(g['measurement_mask'][:])[cols].astype(bool) + ptr = np.asarray(g['indptr'][:], dtype=np.int64) + for a in ['indptr', 'measurement_mask']: + for p in sorted((zp / a / 'c').glob('*')): + if p.is_file(): records.append(receipt(p)) + r = d.shard_row.to_numpy() + size = g['indices'].chunks[0] + assert size == g['data'].chunks[0] == 1000000 + chunks = sorted(set(c for rr in r for c in range(int(ptr[rr]) // size, (int(ptr[rr+1]) - 1) // size + 1))) + paths = [zp / a / 'c' / str(c) for c in chunks for a in ['indices', 'data']] + plans.append({'shard_id': sid, 'cells': len(d), 'chunks': chunks, + 'compressed_bytes': sum(p.stat().st_size for p in paths if p.exists()), + 'decoded_bytes_upper_bound': len(chunks) * size * 12, + 'selected_nnz': int((ptr[r+1]-ptr[r]).sum()), 'measured': mask.tolist(), + 'source_count_matrix': g.attrs['source_count_matrix'], 'source_path': g.attrs['source_path']}) + plan = {'status': 'prepared', 'source_ilc': sum(all_ilc.values()), 'train_ilc': len(v), + 'source_ilc_by_dataset_prefix': all_ilc, 'metadata_shards_scanned': len(files), + 'metadata_rows_scanned': sum(x['metadata_rows'] for x in checks), + 'selected_shards': len(plans), 'compressed_bytes': sum(x['compressed_bytes'] for x in plans), + 'decoded_bytes_upper_bound': sum(x['decoded_bytes_upper_bound'] for x in plans), + 'plans': plans, 'metadata_checks': checks, 'input_sources': records, + 'elapsed_seconds': time.time()-start} + dump('extraction_plan.json', plan) + print(json.dumps({k: plan[k] for k in ['source_ilc', 'train_ilc', 'selected_shards', 'compressed_bytes', 'decoded_bytes_upper_bound', 'elapsed_seconds']}), flush=True) + + +def extract() -> None: + start = time.time() + plan = json.loads((ROOT / 'extraction_plan.json').read_text()) + # Coordinator reviewed the 12.40 GiB cost gate and authorized this exact local subset. + assert plan['compressed_bytes'] <= 13 * 2**30, 'Cost exceeds coordinator-reviewed 12.40 GiB subset' + t = targets(); cols = t.raw_panel_position.to_numpy(dtype=int) + v = pd.read_parquet(ROOT / 'selected_training_ilc.parquet') + out = np.zeros((len(v), len(cols)), dtype=np.int64) + measured = np.zeros_like(out, dtype=bool) + totals = np.zeros(len(v), dtype=np.int64) + chunks_read, shard_checks = [], [] + dataset_timings = []; ds_started = time.time(); ds_bytes = 0; current_ds = None + for pi, pp in enumerate(plan['plans']): + sid = pp['shard_id']; d = v[v.shard_id == sid].sort_values('shard_row') + ds = str(d.dataset_id.iloc[0]) + if current_ds is None: current_ds = ds + elif ds != current_ds: + timing = {'dataset_id': current_ds, 'seconds': time.time()-ds_started, 'compressed_bytes': ds_bytes, + 'process_peak_rss_kib': resource.getrusage(resource.RUSAGE_SELF).ru_maxrss} + dataset_timings.append(timing); print(json.dumps({'dataset_done': timing}), flush=True) + current_ds = ds; ds_started = time.time(); ds_bytes = 0 + zp = BLOCK / 'counts/sc_counts' / (sid + '.zarr') + g = zarr.open_group(str(zp), mode='r') + mask = np.asarray(g['measurement_mask'][:])[cols].astype(bool) + assert mask.tolist() == pp['measured'] + measured[d.index] = mask + ptr = np.asarray(g['indptr'][:], dtype=np.int64) + segs = defaultdict(list); size = 1000000 + for oi, r in zip(d.index, d.shard_row): + a, b = int(ptr[r]), int(ptr[r+1]) + while a < b: + c = a // size; end = min(b, (c+1)*size) + segs[c].append((int(oi), a-c*size, end-c*size)); a = end + assert sorted(segs) == pp['chunks'] + nnz = 0 + for c, pieces in sorted(segs.items()): + a, b = c*size, min((c+1)*size, g['indices'].shape[0]) + ix = np.asarray(g['indices'][a:b], dtype=np.int32) + val = np.asarray(g['data'][a:b]) + assert (val >= 0).all() and np.array_equal(val, val.astype(np.int64)) + val = val.astype(np.int64) + for oi, lo, hi in pieces: + ids, values = ix[lo:hi], val[lo:hi] + totals[oi] += values.sum(); nnz += hi-lo + for j, col in enumerate(cols): out[oi, j] += values[ids == col].sum() + for arr in ['indices', 'data']: + p = zp / arr / 'c' / str(c) + if p.exists(): chunks_read.append(receipt(p)) + assert nnz == pp['selected_nnz'] + shard_checks.append({'shard_id': sid, 'cells': len(d), 'selected_nnz': nnz, 'chunks': len(segs)}) + ds_bytes += pp['compressed_bytes'] + print(f'expression {pi+1}/{len(plan["plans"])} {sid[:6]} cells={len(d)}', flush=True) + assert (totals > 0).all() and (out[~measured] == 0).all() + for j, gid in enumerate(t.gene_id): + v[gid] = out[:, j] + v[gid + '__measured'] = measured[:, j] + v['total_counts_20097'] = totals + v.to_parquet(ROOT / 'training_ilc_marker_counts.parquet', index=False) + dump('expression_chunk_receipts.json', chunks_read) + dataset_timings.append({'dataset_id': current_ds, 'seconds': time.time()-ds_started, 'compressed_bytes': ds_bytes, + 'process_peak_rss_kib': resource.getrusage(resource.RUSAGE_SELF).ru_maxrss}) + dump('extraction_result.json', {'status': 'extracted', 'cells': len(v), 'genes': len(t), + 'elapsed_seconds': time.time()-start, 'shard_checks': shard_checks, + 'process_peak_rss_kib': resource.getrusage(resource.RUSAGE_SELF).ru_maxrss, 'dataset_timings': dataset_timings, + 'chunk_receipts': receipt(ROOT / 'expression_chunk_receipts.json')}) + summarize(v, t) + + +def summarize(v: pd.DataFrame, t: pd.DataFrame) -> None: + tables = {'overall': [], 'by_dataset': [], 'by_donor': []} + grouped = [('overall', {}, v)] + grouped += [('by_dataset', {'dataset_id': k}, d) for k, d in v.groupby('dataset_id', sort=True)] + grouped += [('by_donor', dict(zip(KEY[:2], k)), d) for k, d in v.groupby(KEY[:2], sort=True)] + for level, labels, d in grouped: + for r in t.itertuples(index=False): + eligible = d[r.gene_id + '__measured'] + det = eligible & d[r.gene_id].gt(0) + e, p = d[eligible], d[det] + n = len(e) + tables[level].append({**labels, 'gene_id': r.gene_id, 'gene_symbol': r.gene_symbol, + 'raw_panel_position': int(r.raw_panel_position), 'source_labelled_ilc_cells': len(d), + 'measured_eligible_cells': n, 'unmeasured_cells': len(d)-n, 'detected_cells': len(p), + 'detection_fraction_among_measured': len(p)/n if n else None, + 'total_raw_counts': int(e[r.gene_id].sum()), + 'mean_raw_counts_among_measured': float(e[r.gene_id].mean()) if n else None, + 'measured_dataset_scoped_donors': len(e[KEY[:2]].drop_duplicates()), + 'detected_dataset_scoped_donors': len(p[KEY[:2]].drop_duplicates()), + 'measured_samples': e.sample_key.nunique(), 'detected_samples': p.sample_key.nunique(), + 'in_final6k': bool(r.in_final6k), 'in_7k': bool(r.in_7k), 'in_8k': bool(r.in_8k), + 'sentinel': bool(r.sentinel)}) + for name, rows in tables.items(): pd.DataFrame(rows).to_csv(ROOT / f'marker_support_{name}.csv', index=False) + depths = [] + for name, d in [('ALL', v)] + list(v.groupby('dataset_id', sort=True)): + q = d.total_counts_20097.quantile([0, .1, .25, .5, .75, .9, 1]).to_dict() + depths.append({'dataset_id': name, 'cells': len(d), 'source_donor_ids': d.donor_id.nunique(), + 'samples': d.sample_key.nunique(), **{f'canonical_count_q{k}': x for k, x in q.items()}}) + pd.DataFrame(depths).to_csv(ROOT / 'native_depth_support.csv', index=False) + v.groupby(['dataset_id', 'source_label', 'label_original'], dropna=False).size().rename('cells').reset_index().to_csv(ROOT / 'source_label_support.csv', index=False) + + +def verify() -> None: + t = targets(); cols = t.raw_panel_position.to_numpy(dtype=int); ids = t.gene_id.tolist() + var = pd.read_parquet(E2 / 'metadata/var.parquet') + v = pd.read_parquet(ROOT / 'training_ilc_marker_counts.parquet') + physical, direct, original = [], [], [] + first_ds, boundary_ds = set(), set() + for sid, d in v.groupby('shard_id', sort=True): + zp = BLOCK / 'counts/sc_counts' / (sid + '.zarr') + g = zarr.open_group(str(zp), mode='r') + ep = E2 / 'metadata/obs_sc.parquet' / (sid + '.parquet') + bp = BLOCK / 'metadata/obs_sc.parquet' / (sid + '.parquet') + e = pq.read_table(ep, columns=['row_index', 'source_row_index']).to_pandas() + b = pq.read_table(bp, columns=['row_index', 'source_row_index', 'celltype_l1']).to_pandas() + rows = d.shard_row.to_numpy() + assert np.array_equal(e.iloc[rows].row_index, rows) + assert np.array_equal(e.iloc[rows].source_row_index, d.source_row_index) + assert np.array_equal(b.iloc[rows].source_row_index, d.source_row_index) + assert b.iloc[rows].celltype_l1.astype(str).eq('ILC').all() + assert g.attrs['shape'] == [len(e), len(var)] + physical.append({'shard_id': sid, 'cells': len(d), 'physical_identity_pass': True, + 'block_pre_qc_row_different': int((b.iloc[rows].row_index.to_numpy() != rows).sum())}) + ds = str(d.dataset_id.iloc[0]); chosen = set() + if ds not in first_ds: chosen.update([d.index[0], d.index[-1]]) + ptr = np.asarray(g['indptr'][:]) + boundary = ptr[rows] // 1000000 != (ptr[rows+1]-1) // 1000000 + if ds not in boundary_ds and boundary.any(): + chosen.add(d.index[np.flatnonzero(boundary)[0]]); boundary_ds.add(ds) + for ix in sorted(chosen): + r = v.loc[ix]; a, stop = map(int, ptr[int(r.shard_row):int(r.shard_row)+2]) + ri, rv = np.asarray(g['indices'][a:stop]), np.asarray(g['data'][a:stop]) + observed = np.array([rv[ri == c].sum() for c in cols]) + assert np.array_equal(observed, r[ids].to_numpy(dtype=np.int64)) + assert int(rv.sum()) == int(r.total_counts_20097) + rec = {'dataset_id': ds, 'shard_id': sid, 'shard_row': int(r.shard_row), + 'counts': observed.tolist(), 'row_sum': int(rv.sum()), + 'crosses_chunk_boundary': a // 1000000 != (stop-1) // 1000000} + direct.append(rec) + if ds not in first_ds: + hp = Path('/home/sj_server_1/gblee') / g.attrs['source_path'] + with h5py.File(hp, 'r') as h: + key = g.attrs['source_count_matrix']; vg = h['raw/var' if key.startswith('raw/') else 'var'] + rawids = [x.decode() if isinstance(x, bytes) else str(x) for x in vg[vg.attrs['_index']][:]] + assert len(rawids) == len(set(rawids)) + rawpos = {gid: j for j, gid in enumerate(rawids)}; mx = h[key] + assert mx.attrs['encoding-type'] == 'csr_matrix' + aa, zz = map(int, mx['indptr'][int(r.source_row_index):int(r.source_row_index)+2]) + oi, ov = mx['indices'][aa:zz], mx['data'][aa:zz] + raw_counts = np.array([ov[oi == rawpos[gid]].sum() if gid in rawpos else 0 for gid in ids]) + raw_mask = np.array([gid in rawpos for gid in ids]) + assert np.array_equal(raw_counts, observed) + assert np.array_equal(raw_mask, r[[gid + '__measured' for gid in ids]].to_numpy(dtype=bool)) + canonical = np.array([rawpos[gid] for gid in var.gene_id if gid in rawpos]) + assert ov[np.isin(oi, canonical)].sum() == r.total_counts_20097 + selected_bytes_hash = hashlib.sha256(oi.tobytes() + ov.tobytes()).hexdigest() + original.append({'dataset_id': ds, 'path': str(hp), 'bytes': hp.stat().st_size, + 'mtime_ns': hp.stat().st_mtime_ns, 'matrix': key, 'source_row_index': int(r.source_row_index), + 'shard_id': sid, 'shard_row': int(r.shard_row), '48_counts_match': True, + '48_measurement_mask_matches_original_gene_axis': True, 'canonical_count_sum_matches': True, + 'original_selected_row_indices_values_sha256': selected_bytes_hash, + 'original_gene_axis_sha256': hashlib.sha256('\n'.join(rawids).encode()).hexdigest()}) + first_ds.add(ds) + assert len(original) == v.dataset_id.nunique() == 12 + assert sum(x['cells'] for x in physical) == 16439 + dump('verification.json', {'status': 'PASS', 'physical_rows': physical, 'direct_csr_rows': direct, + 'original_h5ad_rows': original, 'raw_positions': cols.tolist(), 'gene_ids': ids, + 'source_label_is_not_independently_verified_biological_identity': True}) + print(json.dumps({'status': 'PASS', 'cells': len(v), 'shards': len(physical), + 'direct_csr_rows': len(direct), 'original_h5ad_rows': len(original)}), flush=True) + + +if __name__ == '__main__': + ap = argparse.ArgumentParser(); ap.add_argument('stage', choices=['prepare', 'extract', 'verify']) + globals()[ap.parse_args().stage]() diff --git a/paper-releases/pbisc-diffusion/updates/20261015T141849Z-rare-cell-7k-8k-panels/diffusion/dataset/server_1/model8/plans/rare_gene_context_expansion_20261015/coordination/ilc_training_support/extraction_plan.json b/paper-releases/pbisc-diffusion/updates/20261015T141849Z-rare-cell-7k-8k-panels/diffusion/dataset/server_1/model8/plans/rare_gene_context_expansion_20261015/coordination/ilc_training_support/extraction_plan.json new file mode 100644 index 0000000000000000000000000000000000000000..d924629fb20a4bf61d67e81b4503aec98a46a98c --- /dev/null +++ b/paper-releases/pbisc-diffusion/updates/20261015T141849Z-rare-cell-7k-8k-panels/diffusion/dataset/server_1/model8/plans/rare_gene_context_expansion_20261015/coordination/ilc_training_support/extraction_plan.json @@ -0,0 +1,23832 @@ +{ + "status": "prepared", + "source_ilc": 19329, + "train_ilc": 16439, 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