diff --git a/.gitattributes b/.gitattributes index bed0738c7eeb449bca98b5d2f33c89a1ee56349a..4a11dce12b62fba4b06b901d2bd0f98d003df502 100644 --- a/.gitattributes +++ b/.gitattributes @@ -1,60 +1,7 @@ -*.7z filter=lfs diff=lfs merge=lfs -text -*.arrow filter=lfs diff=lfs merge=lfs -text -*.avro filter=lfs diff=lfs merge=lfs -text -*.bin filter=lfs diff=lfs merge=lfs -text -*.bz2 filter=lfs diff=lfs merge=lfs -text -*.ckpt filter=lfs diff=lfs merge=lfs -text -*.ftz filter=lfs diff=lfs merge=lfs -text -*.gz filter=lfs diff=lfs merge=lfs -text -*.h5 filter=lfs diff=lfs merge=lfs -text -*.joblib filter=lfs diff=lfs merge=lfs -text -*.lfs.* filter=lfs diff=lfs merge=lfs -text -*.lz4 filter=lfs diff=lfs merge=lfs -text -*.mds filter=lfs diff=lfs merge=lfs -text -*.mlmodel filter=lfs diff=lfs merge=lfs -text -*.model filter=lfs diff=lfs merge=lfs -text -*.msgpack filter=lfs diff=lfs merge=lfs -text +*.tif filter=lfs diff=lfs merge=lfs -text +*.tiff filter=lfs diff=lfs merge=lfs -text *.npy filter=lfs diff=lfs merge=lfs -text -*.npz filter=lfs diff=lfs merge=lfs -text -*.onnx filter=lfs diff=lfs merge=lfs -text -*.ot filter=lfs diff=lfs merge=lfs -text -*.parquet filter=lfs diff=lfs merge=lfs -text -*.pb filter=lfs diff=lfs merge=lfs -text -*.pickle filter=lfs diff=lfs merge=lfs -text -*.pkl filter=lfs diff=lfs merge=lfs -text +*.zip filter=lfs diff=lfs merge=lfs -text *.pt filter=lfs diff=lfs merge=lfs -text *.pth filter=lfs diff=lfs merge=lfs -text -*.rar filter=lfs diff=lfs merge=lfs -text -*.safetensors filter=lfs diff=lfs merge=lfs -text -saved_model/**/* filter=lfs diff=lfs merge=lfs -text -*.tar.* filter=lfs diff=lfs merge=lfs -text -*.tar filter=lfs diff=lfs merge=lfs -text -*.tflite filter=lfs diff=lfs merge=lfs -text -*.tgz filter=lfs diff=lfs merge=lfs -text -*.wasm filter=lfs diff=lfs merge=lfs -text -*.xz filter=lfs diff=lfs merge=lfs -text -*.zip filter=lfs diff=lfs merge=lfs -text -*.zst filter=lfs diff=lfs merge=lfs -text -*tfevents* filter=lfs diff=lfs merge=lfs -text -# Audio files - uncompressed -*.pcm filter=lfs diff=lfs merge=lfs -text -*.sam filter=lfs diff=lfs merge=lfs -text -*.raw filter=lfs diff=lfs merge=lfs -text -# Audio files - compressed -*.aac filter=lfs diff=lfs merge=lfs -text -*.flac filter=lfs diff=lfs merge=lfs -text -*.mp3 filter=lfs diff=lfs merge=lfs -text -*.ogg filter=lfs diff=lfs merge=lfs -text -*.wav filter=lfs diff=lfs merge=lfs -text -# Image files - uncompressed -*.bmp filter=lfs diff=lfs merge=lfs -text -*.gif filter=lfs diff=lfs merge=lfs -text -*.png filter=lfs diff=lfs merge=lfs -text -*.tiff filter=lfs diff=lfs merge=lfs -text -# Image files - compressed -*.jpg filter=lfs diff=lfs merge=lfs -text -*.jpeg filter=lfs diff=lfs merge=lfs -text -*.webp filter=lfs diff=lfs merge=lfs -text -# Video files - compressed -*.mp4 filter=lfs diff=lfs merge=lfs -text -*.webm filter=lfs diff=lfs merge=lfs -text +allgt_cpsam filter=lfs diff=lfs merge=lfs -text diff --git a/README.md b/README.md index e7d90ddb789e264c5ea4a99dccd0b911883df4c5..2d16d8946edc2053f509988e1cca5d7ccf1b44df 100644 --- a/README.md +++ b/README.md @@ -1,3 +1,48 @@ --- -license: unknown +license: cc-by-4.0 +task_categories: + - image-segmentation + - image-to-text +tags: + - immunofluorescence + - spatial-transcriptomics + - spatial-proteomics + - alzheimers-disease + - cell-segmentation +pretty_name: SPInfer ROSMAP --- + +# SPInfer ROSMAP Data + +This repository is prepared to host the SPInfer ROSMAP data release. + +## Contents + +- `raw_annotations/`: original annotated IF image folders and ROI annotation zip files from the local annotation resource. +- `cellpose_prepared/`: prepared Cellpose-style train/validation/test image and mask folders. +- `metadata/`: donor split file and release metadata. +- `manifest.tsv`: file sizes and SHA256 checksums. + +Clinical/pathology tables are not included in this public staging bundle. + +## Important + +Confirm ROSMAP and institutional data-use permissions before making this repository public. If needed, keep the Hugging Face repository gated. + +## Package + +Use with the SPInfer package: + +```bash +spinfer doctor --config configs/paths.yaml +spinfer prepare-cellpose --config configs/paths.yaml +spinfer infer --config configs/paths.yaml --finetuned-model allgt_cpsam +``` + +## Source + +Local staging was prepared for `liangyou03/SPInfer-ROSMAP`. + +## Citation + +Please cite the SPInfer manuscript and software release when using this dataset. diff --git a/cellpose_prepared/test_cells/gfap_5059.tif b/cellpose_prepared/test_cells/gfap_5059.tif new file mode 100644 index 0000000000000000000000000000000000000000..19a28440f06bb92e48aade8826257c7b93aa3e35 --- /dev/null +++ b/cellpose_prepared/test_cells/gfap_5059.tif @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:0bc61093fd0d603ab3b5972d4505d2bc208d7c8ec4056232b59a30d3a478a33c +size 4330832 diff --git a/cellpose_prepared/test_cells/gfap_5059_mask.tif b/cellpose_prepared/test_cells/gfap_5059_mask.tif new 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0000000000000000000000000000000000000000..ef4dec2368fe5246fb76dcebb728cb18414548d4 --- /dev/null +++ b/metadata/release_metadata.json @@ -0,0 +1,12 @@ +{ + "repo_id": "liangyou03/SPInfer-ROSMAP", + "raw_annotations_source": "/ix/jbwang/liangyou/fintune/data/ann_batch21", + "cellpose_prepared_source": "/ix/jbwang/liangyou/fintune/data/cellpose", + "splits_source": "/ix/jbwang/liangyou/fintune/data/splits", + "notes": [ + "data/rosmap is empty in the current local workspace; cohort raw tiles should be added before public upload if they are intended for release.", + "Prediction outputs under ann_batch21/processed/predictions are intentionally excluded from raw_annotations.", + "Clinical/pathology tables are excluded by default. Rebuild with --include-clinical only after confirming permissions.", + "Confirm ROSMAP data-use permissions before making this dataset public." + ] +} diff --git a/metadata/rosmap_donor_ids_n71.txt b/metadata/rosmap_donor_ids_n71.txt new file mode 100644 index 0000000000000000000000000000000000000000..ef56992d916a113c6601de3b7767455a151b258f --- /dev/null +++ b/metadata/rosmap_donor_ids_n71.txt @@ -0,0 +1,71 @@ +10101327 +10101589 +10203224 +10208143 +10222853 +10249336 +10253148 +10260309 +10271474 +10288185 +10290427 +10291856 +10292311 +10300914 +10315029 +10345561 +10383017 +10394182 +10395197 +10405008 +10424475 +10433070 +10435090 +10436131 +10447620 +10460587 +10473384 +10478041 +10480383 +10490993 +10502798 +10510090 +10516762 +10536568 +10551157 +10557081 +10577337 +10669174 +10684501 +10850171 +10866165 +10885370 +10901987 +10929637 +11157783 +11165535 +11200645 +11259716 +11302830 +11310844 +11319359 +11319389 +11326252 +11327005 +11336574 +11342432 +11345321 +11345331 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data/ann_batch21/processed/for_model/neun_400_cellbodies.npy +neun 568 neun_568 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN568_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_568.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_568_marker.tiff 1040 1388 132 132 209 data/ann_batch21/processed/for_model/neun_568.tif data/ann_batch21/processed/for_model/neun_568_mask.tif data/ann_batch21/processed/for_model/neun_568_cellbodies.npy +neun 585 neun_585 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN585_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_585.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_585_marker.tiff 1040 1388 96 96 215 data/ann_batch21/processed/for_model/neun_585.tif data/ann_batch21/processed/for_model/neun_585_mask.tif data/ann_batch21/processed/for_model/neun_585_cellbodies.npy +neun 635 neun_635 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN635_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_635.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_635_marker.tiff 1040 1388 93 93 51 data/ann_batch21/processed/for_model/neun_635.tif data/ann_batch21/processed/for_model/neun_635_mask.tif data/ann_batch21/processed/for_model/neun_635_cellbodies.npy +neun 888 neun_888 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN888_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_888.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_888_marker.tiff 1040 1388 80 80 22 data/ann_batch21/processed/for_model/neun_888.tif data/ann_batch21/processed/for_model/neun_888_mask.tif data/ann_batch21/processed/for_model/neun_888_cellbodies.npy +neun 1360 neun_1360 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN1360_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_1360.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_1360_marker.tiff 1040 1388 87 87 105 data/ann_batch21/processed/for_model/neun_1360.tif data/ann_batch21/processed/for_model/neun_1360_mask.tif data/ann_batch21/processed/for_model/neun_1360_cellbodies.npy +neun 1492 neun_1492 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN1492_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_1492.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_1492_marker.tiff 1040 1388 60 60 28 data/ann_batch21/processed/for_model/neun_1492.tif data/ann_batch21/processed/for_model/neun_1492_mask.tif data/ann_batch21/processed/for_model/neun_1492_cellbodies.npy +neun 1534 neun_1534 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN1534_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_1534.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_1534_marker.tiff 1040 1388 81 81 23 data/ann_batch21/processed/for_model/neun_1534.tif data/ann_batch21/processed/for_model/neun_1534_mask.tif data/ann_batch21/processed/for_model/neun_1534_cellbodies.npy +neun 2322 neun_2322 Extra NeuN For Liang skipped_missing_image brain_data 0 1 data/ann_batch21/Extra NeuN For Liang/NeuN2322_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_2322.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_2322_marker.tiff +neun 3067 neun_3067 Extra NeuN For Liang included brain_data 1 0 data/ann_batch21/Extra NeuN For Liang/NeuN3067_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_3067.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_3067_marker.tiff 1040 1388 59 59 74 data/ann_batch21/processed/for_model/neun_3067.tif data/ann_batch21/processed/for_model/neun_3067_mask.tif data/ann_batch21/processed/for_model/neun_3067_cellbodies.npy diff --git a/raw_annotations/processed/new_only_definition.md b/raw_annotations/processed/new_only_definition.md new file mode 100644 index 0000000000000000000000000000000000000000..843be691c2a5d31d4e3e396d5d91a809aa4120c6 --- /dev/null +++ b/raw_annotations/processed/new_only_definition.md @@ -0,0 +1,41 @@ +# ann_batch21: New-Only Definition + +Date recorded: 2026-04-22 + +Definition used here: + +- `new` means the `(marker, donor)` pair did **not** already exist in `data/brain_data/*_cellbodies.npy`. +- This is based on comparison against the pre-existing ground-truth set used by the repo before `ann_batch21`. +- For this batch, `new` is a metadata definition, not just "sits inside a new folder". + +Files: + +- Full processed manifest: `data/ann_batch21/processed/manifest.tsv` +- New-only filtered manifest: `data/ann_batch21/processed/new_only_manifest.tsv` + +New rows found in `ann_batch21`: + +- Included in evaluation/model input (`10`, all GFAP): + - `gfap_4192` + - `gfap_4549` + - `gfap_4701` + - `gfap_4764` + - `gfap_5043` + - `gfap_6383` + - `gfap_6487` + - `gfap_6535` + - `gfap_6800` + - `gfap_6838` +- New but not included in evaluation (`1`): + - `neun_2322` + - reason: `skipped_missing_image` because `data/brain_data/neun_2322.tiff` and `data/brain_data/neun_2322_marker.tiff` were not present locally during preprocessing + +Therefore: + +- `new-only` evaluation subset used here = the `10` GFAP samples above +- `new-only` metrics do **not** include `neun_2322` + +New-only metrics on the included `10` samples: + +- `generic_cpsam_l40s_20260422`: precision `0.7321`, recall `0.8362`, F1 `0.7807` +- `allgt_cpsam_l40s_20260422`: precision `0.7143`, recall `0.8405`, F1 `0.7723` diff --git a/raw_annotations/processed/new_only_manifest.tsv b/raw_annotations/processed/new_only_manifest.tsv new file mode 100644 index 0000000000000000000000000000000000000000..a2933d63c5e3ba07ae23a789e6bd115fa1814caf --- /dev/null +++ b/raw_annotations/processed/new_only_manifest.tsv @@ -0,0 +1,12 @@ +marker donor stem source_group status image_source overlap_with_existing_gt is_new_vs_existing_gt roi_zip dapi_source marker_source height width roi_count mask_object_count mask_overlap_pixels for_model_image for_model_mask for_model_cellbodies_npy +gfap 4192 gfap_4192 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_4192/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_4192/Snap-4192_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_4192/Snap-4192_b0c1x0-1388y0-1040.tiff 1040 1388 25 25 0 data/ann_batch21/processed/for_model/gfap_4192.tif data/ann_batch21/processed/for_model/gfap_4192_mask.tif data/ann_batch21/processed/for_model/gfap_4192_cellbodies.npy +gfap 4549 gfap_4549 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_4549/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_4549/Snap-4549_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_4549/Snap-4549_b0c1x0-1388y0-1040.tiff 1040 1388 15 15 0 data/ann_batch21/processed/for_model/gfap_4549.tif data/ann_batch21/processed/for_model/gfap_4549_mask.tif data/ann_batch21/processed/for_model/gfap_4549_cellbodies.npy +gfap 4701 gfap_4701 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_4701/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_4701/Snap-4701_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_4701/Snap-4701_b0c1x0-1388y0-1040.tiff 1040 1388 23 23 0 data/ann_batch21/processed/for_model/gfap_4701.tif data/ann_batch21/processed/for_model/gfap_4701_mask.tif data/ann_batch21/processed/for_model/gfap_4701_cellbodies.npy +gfap 4764 gfap_4764 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_4764/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_4764/Snap-4764_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_4764/Snap-4764_b0c1x0-1388y0-1040.tiff 1040 1388 29 29 6 data/ann_batch21/processed/for_model/gfap_4764.tif data/ann_batch21/processed/for_model/gfap_4764_mask.tif data/ann_batch21/processed/for_model/gfap_4764_cellbodies.npy +gfap 5043 gfap_5043 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_5043/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_5043/Snap-5043_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_5043/Snap-5043_b0c1x0-1388y0-1040.tiff 1040 1388 25 25 0 data/ann_batch21/processed/for_model/gfap_5043.tif data/ann_batch21/processed/for_model/gfap_5043_mask.tif data/ann_batch21/processed/for_model/gfap_5043_cellbodies.npy +gfap 6383 gfap_6383 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_6383/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_6383/Snap-6383_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_6383/Snap-6383_b0c1x0-1388y0-1040.tiff 1040 1388 11 11 0 data/ann_batch21/processed/for_model/gfap_6383.tif data/ann_batch21/processed/for_model/gfap_6383_mask.tif data/ann_batch21/processed/for_model/gfap_6383_cellbodies.npy +gfap 6487 gfap_6487 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_6487/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_6487/Snap-6487_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_6487/Snap-6487_b0c1x0-1388y0-1040.tiff 1040 1388 24 24 0 data/ann_batch21/processed/for_model/gfap_6487.tif data/ann_batch21/processed/for_model/gfap_6487_mask.tif data/ann_batch21/processed/for_model/gfap_6487_cellbodies.npy +gfap 6535 gfap_6535 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_6535/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_6535/Snap-6535_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_6535/Snap-6535_b0c1x0-1388y0-1040.tiff 1040 1388 19 19 0 data/ann_batch21/processed/for_model/gfap_6535.tif data/ann_batch21/processed/for_model/gfap_6535_mask.tif data/ann_batch21/processed/for_model/gfap_6535_cellbodies.npy +gfap 6800 gfap_6800 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_6800/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_6800/Snap-6800_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_6800/Snap-6800_b0c1x0-1388y0-1040.tiff 1040 1388 21 21 0 data/ann_batch21/processed/for_model/gfap_6800.tif data/ann_batch21/processed/for_model/gfap_6800_mask.tif data/ann_batch21/processed/for_model/gfap_6800_cellbodies.npy +gfap 6838 gfap_6838 GFAP_batch2and1 included ann_batch21 0 1 data/ann_batch21/GFAP_batch2and1/GFAP_6838/RoiSet_CellBodies_Final.zip data/ann_batch21/GFAP_batch2and1/GFAP_6838/Snap-6838_b0c0x0-1388y0-1040.tiff data/ann_batch21/GFAP_batch2and1/GFAP_6838/Snap-6838_b0c1x0-1388y0-1040.tiff 1040 1388 40 40 12 data/ann_batch21/processed/for_model/gfap_6838.tif data/ann_batch21/processed/for_model/gfap_6838_mask.tif data/ann_batch21/processed/for_model/gfap_6838_cellbodies.npy +neun 2322 neun_2322 Extra NeuN For Liang skipped_missing_image brain_data 0 1 data/ann_batch21/Extra NeuN For Liang/NeuN2322_RoiSet_CellBodies_IN.zip /ix/jbwang/liangyou/fintune/data/brain_data/neun_2322.tiff /ix/jbwang/liangyou/fintune/data/brain_data/neun_2322_marker.tiff diff --git a/raw_annotations/processed/summary.json b/raw_annotations/processed/summary.json new file mode 100644 index 0000000000000000000000000000000000000000..269e693334c1f91e237515bf3a71b09389f93d2a --- /dev/null +++ b/raw_annotations/processed/summary.json @@ -0,0 +1,19 @@ +{ + "ann_root": "/ix/jbwang/liangyou/fintune/data/ann_batch21", + "out_root": "/ix/jbwang/liangyou/fintune/data/ann_batch21/processed", + "for_model_dir": "/ix/jbwang/liangyou/fintune/data/ann_batch21/processed/for_model", + "n_total": 61, + "n_included": 60, + "n_skipped": 1, + "n_overlap_with_existing_gt": 50, + "n_new_vs_existing_gt": 10, + "included_by_marker": { + "gfap": 40, + "iba1": 11, + "neun": 9 + }, + "skipped_stems": [ + "neun_2322" + ], + "manifest_tsv": "/ix/jbwang/liangyou/fintune/data/ann_batch21/processed/manifest.tsv" +} \ No newline at end of file