String,Scientific pitch,Helmholtz pitch,Interval from middle C,Frequency ( Hz) 1st,E4,e',major third above,329.63 2nd,B3,b,minor second below,246.94 3rd,G3,g,perfect fourth below,196.00 4th,D3,d,minor seventh below,146.83 5th,A2,A,minor tenth below,110.00 6th,E2,E,[MISSING],82.41