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Therefore, reducing the impact of impervious surfaces on surface runoff is an effective approach to alleviate urban waterlogging. Presently, the development mode of many cities in China has shifted from an increase in urban scale to the improvement of urban quality through urban renewal, which is the current and future development path for most cities. Optimizing the design of impervious surfaces in urban renewal planning to reduce its impact on surface runoff is an important way to prevent and control urban waterlogging. The aim of this research is to construct an optimization model of impervious surface space layout under the framework of a geographic simulation technology-integrated ant colony optimization (ACO) and Soil Conservation Service curve number (SCS-CN) model (ACO-SCS) in a case study of Guangzhou in China. Urban runoff plots in the study area are divided according to the area of the urban planning unit. With the goal of minimizing the runoff coefficient, the optimal space layout of the impervious surfaces is obtained, which provides a technical method and reference for urban waterlogging prevention and control through urban renewal planning. The results reveal that the optimization of impervious surface space layout through ACO-SCS achieves a satisfactory effect with an average optimization rate of 9.52%, and a maximum optimization rate of 33.16%. The research also shows that the initial impervious surface layout is the key influencing factor in ACO-SCS. In the urban renewal planning stage, the space layout of the impervious surfaces with a high-low-high density discontinuous connection can be constructed by transforming medium-density impervious surfaces into low-density impervious surfaces to achieve the flat and long-type agglomeration of the low-density and high-density impervious surfaces, which can effectively reduce the influence of urban development on surface runoff. There is spatial heterogeneity of the optimal results in different urban runoff plots. Therefore, the policy of urban renewal planning for urban waterlogging prevention and control should be different. The optimized results of impervious surface space layout provide useful reference information for urban renewal planning.", + "gemini": "Với sự mở rộng nhanh chóng của các bề mặt không thấm nước, tình trạng ngập úng đô thị đã trở thành một \"bệnh đô thị\" điển hình ở Trung Quốc, cản trở nghiêm trọng đến sự phát triển bền vững của các đô thị. Do đó, việc giảm tác động của các bề mặt không thấm nước đối với dòng chảy bề mặt là một cách tiếp cận hiệu quả để giảm ngập úng đô thị. Hiện nay, mô hình phát triển của nhiều thành phố ở Trung Quốc đã chuyển từ tăng quy mô đô thị sang cải thiện chất lượng đô thị thông qua đổi mới đô thị, đây là con đường phát triển hiện tại và tương lai của hầu hết các thành phố. Tối ưu hóa thiết kế các bề mặt không thấm nước trong quy hoạch cải tạo đô thị để giảm tác động của chúng đối với dòng chảy bề mặt là một cách quan trọng để ngăn ngừa và kiểm soát ngập úng đô thị. Mục đích của nghiên cứu này là xây dựng một mô hình tối ưu hóa bố cục không gian bề mặt không thấm nước trong khuôn khổ của công nghệ mô phỏng địa lý tích hợp tối ưu hóa đàn kiến (ACO) và mô hình số đường cong dịch vụ bảo tồn đất (SCS-CN) (ACO-SCS) trong một nghiên cứu trường hợp ở Quảng Châu, Trung Quốc. Các ô dòng chảy đô thị trong khu vực nghiên cứu được phân chia theo diện tích của đơn vị quy hoạch đô thị. Với mục tiêu giảm thiểu hệ số dòng chảy, bố cục không gian tối ưu của các bề mặt không thấm nước được thu được, cung cấp phương pháp kỹ thuật và tài liệu tham khảo để phòng ngừa và kiểm soát ngập úng đô thị thông qua quy hoạch cải tạo đô thị. Kết quả cho thấy việc tối ưu hóa bố cục không gian bề mặt không thấm nước thông qua ACO-SCS đạt hiệu quả khả quan với tỷ lệ tối ưu hóa trung bình là 9,52% và tỷ lệ tối ưu hóa tối đa là 33,16%. Nghiên cứu cũng chỉ ra rằng bố cục bề mặt không thấm nước ban đầu là yếu tố ảnh hưởng chính trong ACO-SCS. Trong giai đoạn quy hoạch cải tạo đô thị, có thể xây dựng bố cục không gian của các bề mặt không thấm nước có kết nối không liên tục mật độ cao-thấp-cao bằng cách chuyển đổi các bề mặt không thấm nước mật độ trung bình thành các bề mặt không thấm nước mật độ thấp để đạt được sự kết tụ phẳng và dài của các bề mặt không thấm nước mật độ thấp và mật độ cao, có thể làm giảm hiệu quả ảnh hưởng của phát triển đô thị đến dòng chảy bề mặt. Có tính không đồng nhất về mặt không gian của các kết quả tối ưu ở các ô dòng chảy đô thị khác nhau. Do đó, chính sách quy hoạch cải tạo đô thị để phòng ngừa và kiểm soát ngập úng đô thị cần phải khác nhau. Các kết quả tối ưu hóa bố cục không gian bề mặt không thấm nước cung cấp thông tin tham khảo hữu ích cho quy hoạch cải tạo đô thị." + }, + { + "idx": 1260001, + "en": "Aphis gossypii Glover (Hemiptera: Aphididae) is a serious polyphagous agricultural pest worldwide. In the present study, we used eight microsatellite markers to investigate the genetic structure and diversity of A. gossypii populations in Korea. Samples were collected from 37 locations in Korea (18 populations in 2016, 14 populations in 2017, and five populations in 2018) from pepper plants. A. gossypii had low to moderate genetic diversity, and expected heterozygosity (HE) ranged from 0.354 to 0.719. A Mantel test of isolation by distance indicated no relationship between genetic structure and geographic distance among all populations (r2 = 0.0004, p = 0.370), suggesting high gene flow among populations in Korea. Populations of A. gossypii in Korea were divided into two distinct genetic clusters (ΔK = 2). In 2016 and 2017, the genetic clusters changed into opposite genetic structures within one year mostly in northwest and southeast parts of Korea. Possible relevance of study results was discussed. Chemical control, cyclical parthenogenesis, and immigrants from the exterior might have resulted in this low genetic diversity and opposite genetic clusters.", + "gemini": "Aphis gossypii Glover (Hemiptera: Aphididae) là một loài gây hại nông nghiệp đa thực nghiêm trọng trên toàn thế giới. Trong nghiên cứu hiện tại, chúng tôi đã sử dụng tám dấu hiệu vi vệ tinh để nghiên cứu cấu trúc và sự đa dạng di truyền của quần thể A. gossypii ở Hàn Quốc. Mẫu được thu thập từ 37 địa điểm ở Hàn Quốc (18 quần thể vào năm 2016, 14 quần thể vào năm 2017 và năm quần thể vào năm 2018) từ cây ớt. A. gossypii có sự đa dạng di truyền từ thấp đến trung bình và độ dị hợp tử mong đợi (HE) dao động từ 0,354 đến 0,719. Kiểm định Mantel về sự cô lập theo khoảng cách không chỉ ra mối quan hệ giữa cấu trúc di truyền và khoảng cách địa lý giữa tất cả các quần thể (r2 = 0,0004, p = 0,370), cho thấy dòng gen cao giữa các quần thể ở Hàn Quốc. Các quần thể A. gossypii ở Hàn Quốc được chia thành hai nhóm di truyền riêng biệt (ΔK = 2). Vào năm 2016 và 2017, các nhóm di truyền đã thay đổi thành các cấu trúc di truyền đối lập trong vòng một năm chủ yếu ở các vùng tây bắc và đông nam của Hàn Quốc. Tầm quan trọng có thể có của kết quả nghiên cứu đã được thảo luận. Kiểm soát hóa học, sinh sản đơn tính theo chu kỳ và những người nhập cư từ bên ngoài có thể dẫn đến sự đa dạng di truyền thấp và các nhóm di truyền đối lập này." + }, + { + "idx": 1260002, + "en": "Mutations of the Telomerase reverse transcriptase (TERT) gene promoter are recurrently found in follicular thyroid carcinoma (FTC) and follicular tumors of uncertain malignant potential (FT-UMP), but nearly never in follicular thyroid adenoma (FTA). We, therefore, believe these mutations could signify malignant potential. At our department, postoperative TERT promoter mutational testing of FT-UMPs was implemented in 2014, with a positive mutation screening leading to vigilant follow-up and sometimes adjuvant treatment. To date, we screened 51 FT-UMPs and compared outcomes to 40 minimally invasive FTCs (miFTCs) with known TERT genotypes. Eight FT-UMPs (16%) displayed TERT promoter mutations, of which four cases underwent a completion lobectomy at the discretion of the patient, and a single patient also opted in for radioiodine (RAI) treatment. Three mutation-positive patients developed distant metastases, registered in one patient receiving a completion lobectomy and in two patients with no additional treatment. Three out of four patients who received additional surgery, including the RAI-treated patient, are still without metastatic disease. We conclude that FT-UMPs with TERT promoter mutations harbor malignant potential and exhibit at least similar recurrence rates to TERT-promoter-mutated miFTCs. Mutational screening should constitute a cornerstone analysis in the histopathological work-up of FT-UMPs.", + "gemini": "Đột biến của gen tiền ung thư Telomerase (TERT) thường được tìm thấy trong ung thư biểu mô tuyến giáp nang (FTC) và các khối u nang có tiềm năng ác tính không chắc chắn (FT-UMP), nhưng hầu như không bao giờ có trong u tuyến giáp nang (FTA). Do đó, chúng tôi tin rằng những đột biến này có thể biểu thị tiềm năng ác tính. Tại khoa của chúng tôi, xét nghiệm đột biến tiền ung thư TERT sau phẫu thuật của FT-UMP đã được thực hiện vào năm 2014, với việc sàng lọc đột biến dương tính dẫn đến theo dõi cảnh giác và đôi khi là điều trị bổ sung. Đến nay, chúng tôi đã sàng lọc 51 FT-UMP và so sánh kết quả với 40 FTC xâm lấn tối thiểu (miFTC) có kiểu gen TERT đã biết. Tám FT-UMP (16%) biểu hiện đột biến tiền ung thư TERT, trong đó bốn trường hợp đã cắt bỏ thùy hoàn toàn theo quyết định của bệnh nhân và một bệnh nhân cũng lựa chọn điều trị iốt phóng xạ (RAI). Ba bệnh nhân có đột biến dương tính đã phát triển di căn xa, được ghi nhận ở một bệnh nhân cắt bỏ thùy hoàn toàn và ở hai bệnh nhân không có thêm điều trị. Ba trong số bốn bệnh nhân được phẫu thuật bổ sung, bao gồm cả bệnh nhân được điều trị bằng RAI, vẫn không bị di căn. Chúng tôi kết luận rằng FT-UMP có đột biến tiền ung thư TERT chứa tiềm năng ác tính và biểu hiện tỷ lệ tái phát ít nhất là tương tự như miFTC có đột biến tiền ung thư TERT. Sàng lọc đột biến nên cấu thành một phân tích nền tảng trong quá trình giải phẫu bệnh của FT-UMP." + }, + { + "idx": 1260003, + "en": "In this study, the shrinkage and creep of concrete containing graphene oxide (GO) nanosheets were experimentally and theoretically investigated. Experiments for the shrinkage and creep of concrete with 0.02% and 0.08% GO nanosheets by the weight of cement and common concrete were carried out. Subsequently, the influence of GO nanosheets on the shrinkage and creep of concrete was analyzed and discussed. A modified model was developed to accurately predict the shrinkage and creep of concrete containing GO nanosheets after models for predicting shrinkage and creep of common concrete were compared and the influential factors and application scope were determined. Results indicate that: (1) GO nanosheets can increase the shrinkage strain and reduce the creep coefficient of concrete, and (2) a modified ACI209 (92) model can accurately predict the shrinkage and creep of concrete containing GO nanosheets. Factors considering concrete strength can be introduced in the model to improve the model accuracy.", + "gemini": "Trong nghiên cứu này, sự co ngót và độ nhớt của bê tông chứa các tấm nano oxit graphene (GO) đã được nghiên cứu thực nghiệm và lý thuyết. Các thí nghiệm về độ co ngót và độ nhớt của bê tông với 0,02% và 0,08% tấm nano GO theo trọng lượng xi măng và bê tông thông thường đã được thực hiện. Sau đó, ảnh hưởng của các tấm nano GO đến độ co ngót và độ nhớt của bê tông đã được phân tích và thảo luận. Một mô hình đã được sửa đổi để dự đoán chính xác độ co ngót và độ nhớt của bê tông chứa các tấm nano GO sau khi các mô hình dự đoán độ co ngót và độ nhớt của bê tông thông thường được so sánh và các yếu tố có ảnh hưởng và phạm vi ứng dụng đã được xác định. Kết quả cho thấy: (1) Các tấm nano GO có thể làm tăng độ co ngót và giảm hệ số độ nhớt của bê tông và (2) mô hình ACI209 (92) đã sửa đổi có thể dự đoán chính xác độ co ngót và độ nhớt của bê tông chứa các tấm nano GO. Các yếu tố xem xét cường độ bê tông có thể được đưa vào mô hình để cải thiện độ chính xác của mô hình." + }, + { + "idx": 1260004, + "en": "Human infections with Brucella melitensis are occasionally reported in Sweden, despite the fact that the national flocks of sheep and goats are officially free from brucellosis. The aim of our study was to analyze 103 isolates of B. melitensis collected from patients in Sweden between 1994 and 2016 and determine their putative geographic origin using whole genome sequencing (WGS)-based tools. The majority of the strains were assigned to East Mediterranean and African lineages. Both in silico Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) and core genome Multilocus Sequence Typing (cgMLST) analyses identified countries of the Middle East as the most probable source of origin of the majority of the strains. Isolates collected from patients with travel history to Iraq or Syria were often associated with genotypes from Turkey, as the cgMLST profiles from these countries clustered together. Sixty strains were located within a distance of 20 core genes to related genotypes from the publicly available database, and for eighteen isolates, the closest genotype was different by more than 50 loci. Our study showed that WGS based tools are effective in tracing back the geographic origin of infection of patients with unknown travel status, provided that public sequences from the location of the source are available.", + "gemini": "Nhiễm trùng ở người do Brucella melitensis đôi khi được báo cáo ở Thụy Điển, mặc dù thực tế là các đàn cừu và dê quốc gia không có bệnh brucella. Mục đích của nghiên cứu của chúng tôi là phân tích 103 phân lập B. melitensis thu thập từ bệnh nhân ở Thụy Điển từ năm 1994 đến năm 2016 và xác định nguồn gốc địa lý có thể của chúng bằng cách sử dụng các công cụ dựa trên giải trình tự toàn bộ hệ gen (WGS). Phần lớn các chủng được phân loại vào các dòng Đông Địa Trung Hải và Châu Phi. Cả phân tích VNTR (Số lượng lặp lại tándem biến đổi) nhiều vị trí trong silico (MLVA) và phân tích gõ trình tự nhiều locus của bộ gen lõi (cgMLST) đều xác định các quốc gia Trung Đông là nguồn gốc có khả năng nhất của phần lớn các chủng. Các phân lập thu thập được từ những bệnh nhân có tiền sử đi du lịch đến Iraq hoặc Syria thường có liên quan đến kiểu gen từ Thổ Nhĩ Kỳ, vì các hồ sơ cgMLST từ các quốc gia này tập hợp lại với nhau. Sáu mươi chủng nằm trong khoảng cách 20 gen lõi so với các kiểu gen liên quan từ cơ sở dữ liệu công khai và đối với mười tám phân lập, kiểu gen gần nhất khác nhau ở hơn 50 locus. Nghiên cứu của chúng tôi cho thấy các công cụ dựa trên WGS có hiệu quả trong việc truy tìm nguồn gốc địa lý của bệnh nhiễm trùng ở những bệnh nhân có tình trạng đi lại không rõ, miễn là có các trình tự công khai từ vị trí của nguồn." + }, + { + "idx": 1260005, + "en": "Balancing the accuracy and speed of three-dimensional (3D) surface measurement of objects is crucial in many important applications. In this paper, we present a wrapped phase and pseudorandom image method and develop an experimental system aiming to avoid the process of phase unwrapping. Our approach can reduce the length of image sequences and improve the speed of pattern projection and image acquisition and can be used as a good candidate for high-speed 3D measurement. The most critical step in our new methodology is using the wrapped phase and the epipolar constraint between one camera and a projector, which can obtain several candidate 3D points within the measurement volume (MV). The false points from the obtained candidate 3D points can be eliminated by the pseudorandom images. A systematic accuracy with MV better than 0.01 mm is achievable. 3D human body measurement results are given to confirm the fast speed of image acquisition capability.", + "gemini": "Việc cân bằng độ chính xác và tốc độ đo bề mặt ba chiều (3D) của vật thể là rất quan trọng trong nhiều ứng dụng quan trọng. Trong bài báo này, chúng tôi trình bày một phương pháp hình ảnh pha được bọc và giả ngẫu nhiên và phát triển một hệ thống thực nghiệm nhằm tránh quá trình mở pha. Phương pháp tiếp cận của chúng tôi có thể làm giảm độ dài của chuỗi hình ảnh và cải thiện tốc độ chiếu mẫu và thu nhận hình ảnh và có thể được sử dụng như một ứng cử viên tốt cho phép đo 3D tốc độ cao. Bước quan trọng nhất trong phương pháp mới của chúng tôi là sử dụng pha được bọc và ràng buộc cực giữa một máy ảnh và một máy chiếu, có thể thu được một số điểm 3D ứng viên trong thể tích đo (MV). Các điểm giả từ các điểm 3D ứng viên thu được có thể bị loại bỏ bởi các hình ảnh giả ngẫu nhiên. Độ chính xác có hệ thống với MV tốt hơn 0,01 mm có thể đạt được. Kết quả đo cơ thể người 3D được đưa ra để xác nhận tốc độ thu nhận hình ảnh nhanh." + }, + { + "idx": 1260006, + "en": "The aims of this study were to determine Cu, Mn, Se, and Zn content in wild mushrooms collected from unpolluted areas of the eastern Green Lungs of Poland (GLP) territory, to compare them to some popular species of cultivated mushrooms, evaluate mushroom contribution to the daily intake of the studied bioelements, and to determine their possible toxic effect resulting from potentially excessive mushroom consumption from areas recognized as ecologically uncontaminated. Bioelements were determined in 21 species of edible mushrooms: eighteen species of wild mushrooms and three species of popular cultivated mushrooms. The mean Cu, Mn, Se, and Zn content (in µg/g, dry mass DM) ranged from 10.6-123.1, 12.2-41, 0.13-13.3, and 68.3-184, respectively. A comparison with recommended dietary allowance (RDA) for Cu, Se, and Zn as well as adequate intake (AI) for Mn demonstrated that a 100 g fresh mass (FM) portion of mushroom species with the highest content of a given element can meet the demand for Cu, Mn, Se and Zn at 203%, 14-17%, 211%, and 16-22%, respectively. A comparison of the content of the examined bioelements contained in one portion of mushrooms (100 g FM) against the toxicological intake limits for different chemical elements with the provisional maximum tolerable daily intake (PMTDI) and upper intake level (UL) showed no risk of toxicity for the evaluated mushroom species.", + "gemini": "Mục đích của nghiên cứu này là xác định hàm lượng Cu, Mn, Se và Zn trong nấm hoang thu thập từ các khu vực không bị ô nhiễm của lãnh thổ Phổi xanh phía đông Ba Lan (GLP), để so sánh chúng với một số loài nấm được nuôi trồng phổ biến, đánh giá sự đóng góp của nấm vào lượng hấp thụ sinh học hàng ngày các nguyên tố sinh học đã nghiên cứu và xác định tác dụng độc hại có thể có của chúng do tiêu thụ quá nhiều nấm từ các khu vực được công nhận là không bị ô nhiễm về mặt sinh thái. Các nguyên tố sinh học đã được xác định trong 21 loài nấm ăn được: mười tám loài nấm hoang và ba loài nấm được nuôi trồng phổ biến. Hàm lượng Cu, Mn, Se và Zn trung bình (tính theo µg/g, khối lượng khô DM) dao động từ 10,6-123,1, 12,2-41, 0,13-13,3 và 68,3-184. Khi so sánh với chế độ ăn uống khuyến nghị (RDA) đối với Cu, Se và Zn cũng như lượng hấp thụ đầy đủ (AI) đối với Mn, kết quả cho thấy một khẩu phần 100 g khối lượng tươi (FM) của loài nấm có hàm lượng cao nhất của một nguyên tố nhất định có thể đáp ứng nhu cầu về Cu, Mn, Se và Zn ở mức 203%, 14-17%, 211% và 16-22%. Khi so sánh hàm lượng các nguyên tố sinh học đã kiểm tra có trong một khẩu phần nấm (100 g FM) với giới hạn hấp thụ độc tính đối với các nguyên tố hóa học khác nhau với lượng hấp thụ tối đa tạm thời có thể dung nạp hàng ngày (PMTDI) và mức hấp thụ trên (UL) cho thấy không có nguy cơ độc tính đối với các loài nấm đã đánh giá." + }, + { + "idx": 1260007, + "en": "Passengers travelling on the London underground tubes currently have no means of knowing their whereabouts between stations. The challenge for providing such service is that the London underground tunnels have no GPS, Wi-Fi, Bluetooth, or any kind of terrestrial signals to leverage. This paper presents a novel yet practical idea to track passengers in realtime using the smartphone accelerometer and a training database of the entire London underground network. Our rationales are that London tubes are self-driving transports with predictable accelerations, decelerations, and travelling time and that they always travel on the same fixed rail lines between stations with distinctive bumps and vibrations, which permit us to generate an accelerometer map of the tubes' movements on each line. Given the passenger's accelerometer data, we identify in realtime what line they are travelling on and what station they depart from, using a pattern-matching algorithm, with an accuracy of up to about 90% when the sampling length is equivalent to at least 3 station stops. We incorporate Principal Component Analysis to perform inertial tracking of passengers' positions along the line when trains break away from scheduled movements during rush hours. Our proposal was painstakingly assessed on the entire London underground, covering approximately 940 km of travelling distance, spanning across 381 stations on 11 different lines.", + "gemini": "Hành khách đi trên tàu điện ngầm London hiện không có cách nào để biết vị trí của mình giữa các ga. Thách thức trong việc cung cấp dịch vụ như vậy là các đường hầm tàu điện ngầm London không có GPS, Wi-Fi, Bluetooth hoặc bất kỳ loại tín hiệu mặt đất nào để tận dụng. Bài báo này trình bày một ý tưởng mới nhưng thiết thực để theo dõi hành khách theo thời gian thực bằng cách sử dụng gia tốc kế điện thoại thông minh và cơ sở dữ liệu đào tạo của toàn bộ mạng lưới tàu điện ngầm London. Cơ sở lý luận của chúng tôi là các tuyến tàu điện ngầm London là phương tiện tự lái với khả năng tăng tốc, giảm tốc và thời gian di chuyển có thể dự đoán được và chúng luôn đi trên cùng một tuyến đường ray cố định giữa các ga với những va chạm và rung động đặc biệt, cho phép chúng tôi tạo ra một bản đồ gia tốc về chuyển động của các tuyến tàu trên mỗi tuyến. Với dữ liệu gia tốc kế của hành khách, chúng tôi xác định theo thời gian thực tuyến nào họ đang đi và họ khởi hành từ ga nào, bằng cách sử dụng thuật toán khớp mẫu, với độ chính xác lên đến khoảng 90% khi độ dài lấy mẫu tương đương với ít nhất 3 điểm dừng của ga. Chúng tôi kết hợp Phân tích thành phần chính để thực hiện theo dõi quán tính vị trí của hành khách dọc theo tuyến khi tàu hỏa thoát khỏi các chuyển động theo lịch trình trong giờ cao điểm. Đề xuất của chúng tôi đã được đánh giá cẩn thận trên toàn bộ tàu điện ngầm London, bao phủ khoảng 940 km khoảng cách di chuyển, trải dài trên 381 ga trên 11 tuyến khác nhau." + }, + { + "idx": 1260008, + "en": "Brain health refers to the preservation of brain integrity and function optimized for an individual's biological age. Several studies have demonstrated that our lifestyles habits impact our brain health and our cognitive and mental wellbeing. Monitoring such lifestyles is thus critical and mobile technologies are essential to enable such a goal. Three databases were selected to carry out the search. Then, a PRISMA and PICOTS based criteria for a more detailed review on the basis of monitoring lifestyle aspects were used to filter the publications. We identified 133 publications after removing duplicates. Fifteen were finally selected from our criteria. Many studies still use questionnaires as the only tool for monitoring and do not apply advanced analytic or AI approaches to fine-tune results. We anticipate a transformative boom in the near future developing and implementing solutions that are able to integrate, in a flexible and adaptable way, data from technologies and devices that users might already use. This will enable continuous monitoring of objective data to guide the personalized definition of lifestyle goals and data-driven coaching to offer the necessary support to ensure adherence and satisfaction.", + "gemini": "Sức khỏe não bộ đề cập đến việc bảo tồn tính toàn vẹn và chức năng của não được tối ưu hóa cho độ tuổi sinh học của một cá nhân. Một số nghiên cứu đã chứng minh rằng thói quen lối sống của chúng ta ảnh hưởng đến sức khỏe não bộ và sức khỏe nhận thức và tinh thần của chúng ta. Do đó, việc theo dõi các lối sống như vậy là rất quan trọng và các công nghệ di động rất cần thiết để đạt được mục tiêu đó. Ba cơ sở dữ liệu đã được chọn để thực hiện tìm kiếm. Sau đó, một tiêu chí dựa trên PRISMA và PICOTS để đánh giá chi tiết hơn về cơ sở theo dõi các khía cạnh lối sống đã được sử dụng để lọc các ấn phẩm. Chúng tôi đã xác định được 133 ấn phẩm sau khi loại bỏ các bản trùng lặp. Cuối cùng, mười lăm ấn phẩm đã được chọn từ các tiêu chí của chúng tôi. Nhiều nghiên cứu vẫn sử dụng bảng câu hỏi làm công cụ duy nhất để theo dõi và không áp dụng các phương pháp phân tích nâng cao hoặc AI để tinh chỉnh kết quả. Chúng tôi dự đoán một sự bùng nổ mang tính biến đổi trong tương lai gần, phát triển và triển khai các giải pháp có khả năng tích hợp, theo cách linh hoạt và có thể thích ứng, dữ liệu từ các công nghệ và thiết bị mà người dùng có thể đã sử dụng. Điều này sẽ cho phép theo dõi liên tục dữ liệu khách quan để hướng dẫn định nghĩa được cá nhân hóa về các mục tiêu lối sống và hướng dẫn dựa trên dữ liệu để đưa ra sự hỗ trợ cần thiết nhằm đảm bảo tính tuân thủ và sự hài lòng." + }, + { + "idx": 1260009, + "en": "We present a model that estimates the spectral phytoplankton absorption coefficient ( a p h ( λ ) ) of four phytoplankton groups (picophytoplankton, nanophytoplankton, dinoflagellates, and diatoms) as a function of the total chlorophyll-a concentration (C) and sea surface temperature (SST). Concurrent data on a p h ( λ ) (at 12 visible wavelengths), C and SST, from the surface layer (<20 m depth) of the North Atlantic Ocean, were partitioned into training and independent validation data, the validation data being matched with satellite ocean-colour observations. Model parameters (the chlorophyll-specific phytoplankton absorption coefficients of the four groups) were tuned using the training data and found to compare favourably (in magnitude and shape) with results of earlier studies. Using the independent validation data, the new model was found to retrieve total a p h ( λ ) with a similar performance to two earlier models, using either in situ or satellite data as input. Although more complex, the new model has the advantage of being able to determine a p h ( λ ) for four phytoplankton groups and of incorporating the influence of SST on the composition of the four groups. We integrate the new four-population absorption model into a simple model of ocean colour, to illustrate the influence of changes in SST on phytoplankton community structure, and consequently, the blue-to-green ratio of remote-sensing reflectance. We also present a method of propagating error through the model and illustrate the technique by mapping errors in group-specific a p h ( λ ) using a satellite image. We envisage the model will be useful for ecosystem model validation and assimilation exercises and for investigating the influence of temperature change on ocean colour.", + "gemini": "Chúng tôi trình bày một mô hình ước tính hệ số hấp thụ quang phổ của thực vật phù du (a p h (λ)) của bốn nhóm thực vật phù du (thực vật phù du nhỏ, thực vật phù du nano, tảo giáp và tảo cát) như một hàm của tổng nồng độ chlorophyll-a (C) và nhiệt độ bề mặt biển (SST). Dữ liệu đồng thời về a p h (λ) (ở 12 bước sóng khả kiến), C và SST, từ lớp bề mặt (<20 m độ sâu) của Bắc Đại Tây Dương, được phân thành dữ liệu đào tạo và dữ liệu xác thực độc lập, dữ liệu xác thực được khớp với các quan sát màu đại dương vệ tinh. Các tham số mô hình (các hệ số hấp thụ quang phổ của thực vật phù du đặc hiệu của chlorophyll của bốn nhóm) được điều chỉnh bằng cách sử dụng dữ liệu đào tạo và được phát hiện là so sánh thuận lợi (về độ lớn và hình dạng) với kết quả của các nghiên cứu trước đó. Sử dụng dữ liệu xác thực độc lập, mô hình mới được phát hiện để thu hồi tổng a p h (λ) với hiệu suất tương tự như hai mô hình trước đó, sử dụng dữ liệu tại chỗ hoặc vệ tinh làm đầu vào. Mặc dù phức tạp hơn, mô hình mới có ưu điểm là có thể xác định a p h (λ) cho bốn nhóm thực vật phù du và kết hợp ảnh hưởng của SST vào thành phần của bốn nhóm. Chúng tôi tích hợp mô hình hấp thụ bốn quần thể mới vào một mô hình đơn giản về màu đại dương, để minh họa ảnh hưởng của những thay đổi trong SST đối với cấu trúc quần xã thực vật phù du và do đó, tỷ lệ phản xạ cảm biến từ xa từ xanh lam sang xanh lục. Chúng tôi cũng trình bày một phương pháp lan truyền lỗi thông qua mô hình và minh họa kỹ thuật bằng cách lập bản đồ lỗi trong a p h (λ) theo nhóm cụ thể bằng cách sử dụng hình ảnh vệ tinh. Chúng tôi hình dung rằng mô hình sẽ hữu ích cho các bài tập xác thực và đồng hóa mô hình hệ sinh thái và để điều tra ảnh hưởng của sự thay đổi nhiệt độ đối với màu đại dương." + }, + { + "idx": 1260010, + "en": "Obsessive‒compulsive disorder (OCD), and especially contamination obsessions and washing compulsions, has been related to disgust. However, when its cardiovascular correlates have been studied, contradictory results have been found, including heart rate accelerations and decelerations. The aim of this study is to analyze emotional, cognitive, and cardiovascular responses in nonclinical (control) and subclinical participants with obsessive‒compulsive contamination/washing symptoms when confronted with a disgusting stimulus. Twenty-seven participants (14 subclinical OCD) completed a behavioral avoidance task with a contamination-based stimulus while their heart rate and subjective variables were measured. Results showed heart rate reductions in both samples, whereas subjective measures reflected higher disgust, anxiety, dirtiness, and emotional valence in the subclinical sample. However, at the same time, the sense of dominance was lower in the control group. In conclusion, our results support a heart rate deceleration during exposure to a disgusting stimulus dissociated from the subjective experience.", + "gemini": "Rối loạn ám ảnh cưỡng chế (OCD), và đặc biệt là ám ảnh về sự ô nhiễm và hành vi rửa trôi, có liên quan đến sự ghê tởm. Tuy nhiên, khi các tương quan về tim mạch của nó được nghiên cứu, người ta đã tìm thấy những kết quả trái ngược nhau, bao gồm cả sự tăng và giảm nhịp tim. Mục đích của nghiên cứu này là phân tích các phản ứng về cảm xúc, nhận thức và tim mạch ở những người không mắc bệnh (nhóm chứng) và những người mắc các triệu chứng ám ảnh cưỡng chế về ô nhiễm/rửa trôi ở mức độ cận lâm sàng khi đối mặt với một kích thích gây ghê tởm. Hai mươi bảy người tham gia (14 người mắc OCD cận lâm sàng) đã hoàn thành nhiệm vụ tránh hành vi với một kích thích dựa trên sự ô nhiễm trong khi nhịp tim và các biến số chủ quan của họ được đo. Kết quả cho thấy nhịp tim giảm ở cả hai nhóm mẫu, trong khi các biện pháp chủ quan phản ánh mức độ ghê tởm, lo lắng, bẩn thỉu và giá trị cảm xúc cao hơn ở nhóm cận lâm sàng. Tuy nhiên, đồng thời, cảm giác thống trị thấp hơn ở nhóm chứng. Tóm lại, kết quả của chúng tôi ủng hộ sự giảm nhịp tim trong quá trình tiếp xúc với một kích thích gây ghê tởm tách biệt khỏi trải nghiệm chủ quan." + }, + { + "idx": 1260011, + "en": "Corynespora leaf spot caused by Corynespora cassiicola is one of the major diseases in cucumber (Cucumis sativus L.). However, the resistance mechanisms and signals of cucumber to C. cassiicola are unclear. Here, we report that the mildew resistance locus O (MLO) genes, CsMLO1 and CsMLO2, are both negative modulators of the cucumber defense response to C. cassiicola. Subcellular localization analysis showed that CsMLO1 and CsMLO2 are localized in the plasma membrane. Expression analysis indicated that the transcript levels of CsMLO1 and CsMLO2 are linked to the defense response to C. cassiicola. Transient overexpression of either CsMLO1 or CsMLO2 in cucumber cotyledons reduced resistance to C. cassiicola, whereas silencing of either CsMLO1 or CsMLO2 enhanced resistance to C. cassiicola. The relationships of pathogenesis-related proteins, reactive oxygen species (ROS)-associated genes, and abscisic acid (ABA)-related genes to the overexpression and silencing of CsMLO1/CsMLO2 in non-infested cucumber plants were investigated. The results indicated that CsMLO1 mediated resistance against C. cassiicola by regulating the expression of pathogenesis-related proteins and ROS-associated genes, as well as through ABA signaling pathway-associated genes. The CsMLO2-mediated resistance against C. cassiicola primarily involves regulation of the expression of pathogenesis-related proteins. Our findings will guide strategies to enhance the resistance of cucumber to corynespora leaf spot.", + "gemini": "Bệnh đốm lá Corynespora do nấm Corynespora cassiicola gây ra là một trong những bệnh chính ở dưa chuột (Cucumis sativus L.). Tuy nhiên, cơ chế kháng bệnh và tín hiệu của dưa chuột đối với C. cassiicola vẫn chưa rõ ràng. Ở đây, chúng tôi báo cáo rằng các gen MLO (mildew resistance locus O), CsMLO1 và CsMLO2, đều là những chất điều hòa tiêu cực của phản ứng phòng vệ của dưa chuột đối với C. cassiicola. Phân tích định vị dưới tế bào cho thấy CsMLO1 và CsMLO2 được định vị ở màng tế bào chất. Phân tích biểu hiện cho thấy mức phiên mã của CsMLO1 và CsMLO2 có liên quan đến phản ứng phòng vệ đối với C. cassiicola. Sự biểu hiện quá mức tạm thời của CsMLO1 hoặc CsMLO2 ở lá mầm dưa chuột làm giảm khả năng kháng bệnh đối với C. cassiicola, trong khi việc làm im lặng CsMLO1 hoặc CsMLO2 đều làm tăng khả năng kháng bệnh đối với C. cassiicola. Mối quan hệ của các protein liên quan đến sinh bệnh, các gen liên quan đến các loài oxy phản ứng (ROS) và các gen liên quan đến axit abscisic (ABA) với sự biểu hiện quá mức và im lặng của CsMLO1/CsMLO2 ở cây dưa chuột không bị nhiễm đã được nghiên cứu. Kết quả chỉ ra rằng CsMLO1 đã trung gian khả năng kháng bệnh đối với C. cassiicola bằng cách điều chỉnh biểu hiện của các protein liên quan đến sinh bệnh và các gen liên quan đến ROS, cũng như thông qua các gen liên quan đến con đường truyền tín hiệu ABA. Khả năng kháng bệnh do CsMLO2 trung gian đối với C. cassiicola chủ yếu liên quan đến việc điều chỉnh biểu hiện của các protein liên quan đến sinh bệnh. Những phát hiện của chúng tôi sẽ hướng dẫn các chiến lược để tăng cường khả năng kháng bệnh của dưa chuột đối với bệnh đốm lá corynespora." + }, + { + "idx": 1260015, + "en": "Chronic obstructive pulmonary disease (COPD) accounts for the largest proportion of respiratory deaths worldwide and was historically the leading indication for lung transplantation. The success of lung transplantation procedures is measured as survival benefit, calculated as survival with transplantation minus predicted survival without transplantation. In chronic obstructive pulmonary disease, it is difficult to show a clear and consistent survival benefit. Increasing knowledge of the risk factors, phenotypical heterogeneity, systemic manifestations, and their management helps improve our ability to select candidates and list those that will benefit the most from the procedure.", + "gemini": "Bệnh phổi tắc nghẽn mạn tính (COPD) chiếm tỷ lệ tử vong do hô hấp lớn nhất trên toàn thế giới và trong lịch sử là chỉ định hàng đầu cho ghép phổi. Sự thành công của các thủ thuật ghép phổi được đo bằng lợi ích về sự sống còn, được tính bằng sự sống còn sau ghép trừ đi sự sống còn dự đoán mà không ghép. Trong bệnh phổi tắc nghẽn mạn tính, rất khó để chứng minh lợi ích rõ ràng và nhất quán về sự sống còn. Việc tăng cường hiểu biết về các yếu tố nguy cơ, tính không đồng nhất về kiểu hình, biểu hiện toàn thân và cách quản lý của chúng giúp cải thiện khả năng lựa chọn ứng viên và liệt kê những người sẽ được hưởng lợi nhiều nhất từ thủ thuật này." + }, + { + "idx": 1260016, + "en": "A heatsink is a large experimental device which is used to simulate the outer space environment. In this paper, a Raman-based distributed temperature sensor was used for real-time and continuous heatsink temperature monitoring, and a special Raman-based distributed temperature sensing method and system have been proposed. This method takes advantage of three calibration parameters ( Δ α , γ , C ) to calculate the temperature. These three parameters are related to the attenuation of the optical fiber, the Raman translation, and the difference of optoelectronic conversion, respectively. Optical time domain reflectometry was used to calculate the location. A series of heatsink temperature measurement experiments were performed in a vacuum and -173 °C environment. When the temperature dropped to -100 °C, the parameter Δ α was found to vary. A method was proposed to recalculate Δ α and modify the traditional Raman fiber temperature equation. The results of the experiments confirmed the validity of this modified Raman fiber temperature equation. Based on this modified equation, the temperature field in the heatsink was calculated. The Raman-based distributed temperature sensor has potential applications in temperature measurement and judging the occurrence of faults in space exploration.", + "gemini": "Tản nhiệt là một thiết bị thử nghiệm lớn được sử dụng để mô phỏng môi trường không gian bên ngoài. Trong bài báo này, một cảm biến nhiệt độ phân tán dựa trên Raman đã được sử dụng để theo dõi nhiệt độ tản nhiệt liên tục và thời gian thực, và một phương pháp và hệ thống cảm biến nhiệt độ phân tán dựa trên Raman đặc biệt đã được đề xuất. Phương pháp này tận dụng ba tham số hiệu chuẩn ( Δ α , γ , C ) để tính nhiệt độ. Ba tham số này lần lượt liên quan đến sự suy giảm của sợi quang, sự dịch chuyển Raman và sự khác biệt về chuyển đổi quang điện. Phản xạ kế miền thời gian quang học được sử dụng để tính vị trí. Một loạt các thí nghiệm đo nhiệt độ tản nhiệt đã được thực hiện trong môi trường chân không và -173 °C. Khi nhiệt độ giảm xuống -100 °C, người ta thấy tham số Δ α thay đổi. Một phương pháp đã được đề xuất để tính toán lại Δ α và sửa đổi phương trình nhiệt độ sợi Raman truyền thống. Kết quả của các thí nghiệm đã xác nhận tính hợp lệ của phương trình nhiệt độ sợi Raman đã sửa đổi này. Dựa trên phương trình đã sửa đổi này, trường nhiệt độ trong tản nhiệt đã được tính toán. Cảm biến nhiệt độ phân tán dựa trên Raman có các ứng dụng tiềm năng trong việc đo nhiệt độ và đánh giá sự xuất hiện của các lỗi trong quá trình thám hiểm không gian." + }, + { + "idx": 1260017, + "en": "Atherosclerosis is a major cardiovascular disease worldwide, that could benefit from innovative nanomedicine imaging tools and treatments. In this perspective, we here studied, by fluorescence imaging in ApoE-/- mice, the biodistribution of non-functionalized and RXP470.1-targeted nanostructured lipid carriers (NLC) loaded with DiD dye. RXP470.1 specifically binds to MMP12, a metalloprotease that is over-expressed by macrophages residing in atherosclerotic plaques. Physico-chemical characterizations showed that RXP-NLC (about 105 RXP470.1 moieties/particle) displayed similar features as non-functionalized NLC in terms of particle diameter (about 60-65 nm), surface charge (about -5 - -10 mV), and colloidal stability. In vitro inhibition assays demonstrated that RXP-NLC conserved a selectivity and affinity profile, which favored MMP-12. In vivo data indicated that NLC and RXP-NLC presented prolonged blood circulation and accumulation in atherosclerotic lesions in a few hours. Twenty-four hours after injection, particle uptake in atherosclerotic plaques of the brachiocephalic artery was similar for both nanoparticles, as assessed by ex vivo imaging. This suggests that the RXP470.1 coating did not significantly induce an active targeting of the nanoparticles within the plaques. Overall, NLCs appeared to be very promising nanovectors to efficiently and specifically deliver imaging agents or drugs in atherosclerotic lesions, opening avenues for new nanomedicine strategies for cardiovascular diseases.", + "gemini": "Xơ vữa động mạch là một bệnh tim mạch chính trên toàn thế giới, có thể được hưởng lợi từ các công cụ và phương pháp điều trị hình ảnh nano y học sáng tạo. Theo quan điểm này, chúng tôi đã nghiên cứu ở đây, bằng hình ảnh huỳnh quang ở chuột ApoE-/- , sự phân bố sinh học của các chất mang lipid có cấu trúc nano không chức năng và nhắm mục tiêu RXP470.1 (NLC) được tải với thuốc nhuộm DiD. RXP470.1 liên kết đặc hiệu với MMP12, một metalloprotease được biểu hiện quá mức bởi các đại thực bào cư trú trong các mảng xơ vữa động mạch. Các đặc điểm vật lý-hóa học cho thấy RXP-NLC (khoảng 105 nhóm RXP470.1/hạt) biểu hiện các đặc điểm tương tự như NLC không chức năng về đường kính hạt (khoảng 60-65 nm), điện tích bề mặt (khoảng -5 - -10 mV) và độ ổn định keo. Các xét nghiệm ức chế trong ống nghiệm chứng minh rằng RXP-NLC bảo toàn cấu hình chọn lọc và ái lực, có lợi cho MMP-12. Dữ liệu trong ống nghiệm chỉ ra rằng NLC và RXP-NLC có sự lưu thông máu kéo dài và tích tụ trong các tổn thương xơ vữa động mạch sau vài giờ. Hai mươi bốn giờ sau khi tiêm, sự hấp thụ hạt trong các mảng xơ vữa động mạch của động mạch não chủ là tương tự đối với cả hai loại hạt nano, như được đánh giá bằng hình ảnh ngoại sinh. Điều này cho thấy lớp phủ RXP470.1 không tạo ra đáng kể sự nhắm mục tiêu tích cực của các hạt nano trong các mảng bám. Nhìn chung, NLC dường như là các hạt nano rất hứa hẹn để đưa hiệu quả và đặc hiệu các tác nhân tạo ảnh hoặc thuốc vào các tổn thương xơ vữa động mạch, mở ra con đường cho các chiến lược nano y học mới cho các bệnh tim mạch." + } +] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_126/data.json.error b/retranslated_vi_pubmed old format/chunk_126/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..060db94102072458bd347ec080bbac28f998cc00 --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_126/data.json.error @@ -0,0 +1,11 @@ +[ + 1260012, + 1260013, + 1260014, + 1260018, + 1260019, + 1260020, + 1260021, + 1260022, + 1260023 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_13/data.json b/retranslated_vi_pubmed old format/chunk_13/data.json new file mode 100644 index 0000000000000000000000000000000000000000..8eedcc6bd627254fe9e443efe4105b4d3439e3d2 --- /dev/null +++ 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0000000000000000000000000000000000000000..f7fc9ba83eb65c0dfc0c37a986bd8aa04396505c --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_19/data.json.error @@ -0,0 +1,8 @@ +[ + 191792, + 192156, + 193450, + 195731, + 196983, + 198121 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_2/data.json b/retranslated_vi_pubmed old format/chunk_2/data.json new file mode 100644 index 0000000000000000000000000000000000000000..c48a5e949236fd5e005837d2e2cc2b96a087130f --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_2/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:6926d42a1412df80cd119a0f0d3b42744fa8a34036aa85d33bfa7af91f1230c2 +size 23921135 diff --git a/retranslated_vi_pubmed old format/chunk_2/data.json.error b/retranslated_vi_pubmed old format/chunk_2/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..82645cca510859a0a2257be55e9ee5ddabfe144b --- /dev/null +++ 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newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_21/data.json b/retranslated_vi_pubmed old format/chunk_21/data.json new file mode 100644 index 0000000000000000000000000000000000000000..1f8cf8a11345273f03562dfd7744e51a2e9908de --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_21/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:210b87c89060a5d898538bf5a951f65a23f12aa202e251cba69b47797e5c5c71 +size 29158596 diff --git a/retranslated_vi_pubmed old format/chunk_21/data.json.error b/retranslated_vi_pubmed old format/chunk_21/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_21/data.json.error @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_22/data.json b/retranslated_vi_pubmed old format/chunk_22/data.json new file mode 100644 index 0000000000000000000000000000000000000000..209f9804fca10da08f217de274aae8149b9aea48 --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_22/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:78a5ae65a3c893833ef60c783d0e848b2fc9f707f1bac1945e401bf7373ad55a +size 19632931 diff --git a/retranslated_vi_pubmed old format/chunk_22/data.json.error b/retranslated_vi_pubmed old format/chunk_22/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..dd85ed261ca35c8ff870abb76067bf1076f4496a --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_22/data.json.error @@ -0,0 +1,53 @@ +[ + 220118, + 220466, + 220470, + 221257, + 221796, + 221878, + 221879, + 221884, + 221976, + 222081, + 222110, + 222117, + 222125, + 222156, + 222185, + 222225, + 222233, + 222449, + 222466, + 222555, + 222561, + 222636, + 222822, + 222823, + 222956, + 223952, + 224024, + 224065, + 224075, + 225001, + 225008, + 225460, + 225667, + 225723, + 225728, 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0000000000000000000000000000000000000000..baf3dc03e1ad63c9e4a57f8382eaf8d875a300da --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_26/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:b8eb422f5d624003f7ba073157a786c9fd32394835bdb1abd4bd6a295881c76b +size 28744002 diff --git a/retranslated_vi_pubmed old format/chunk_26/data.json.error b/retranslated_vi_pubmed old format/chunk_26/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..03e8e0277b61f03a905f6f9c2646c0bcf981119c --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_26/data.json.error @@ -0,0 +1,11 @@ +[ + 260430, + 261677, + 261911, + 263030, + 263038, + 264950, + 266218, + 268135, + 269659 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_27/data.json b/retranslated_vi_pubmed old format/chunk_27/data.json new file mode 100644 index 0000000000000000000000000000000000000000..b4f1900934694347eee649bac8955bc5dc6610fa --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_27/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:7122874b094507dd9da5fd3ede9994a7eb591e88291989b3ebff82cbdd3d1063 +size 30423197 diff --git a/retranslated_vi_pubmed old format/chunk_27/data.json.error b/retranslated_vi_pubmed old format/chunk_27/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..aee285950e1a24c45b2531e3bc0a003c67430654 --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_27/data.json.error @@ -0,0 +1,5 @@ +[ + 272607, + 272898, + 272917 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_28/data.json b/retranslated_vi_pubmed old format/chunk_28/data.json new file mode 100644 index 0000000000000000000000000000000000000000..cd6799bf1111f73904b92b37f901319cd7ba84e9 --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_28/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:386040968e0fb03a90835740bf8cd05a3d14efdb0815410b8b1004c6e57d5137 +size 31682987 diff --git a/retranslated_vi_pubmed old format/chunk_28/data.json.error b/retranslated_vi_pubmed old format/chunk_28/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..ad29d1890faa723b5e33bd42c12fef58ad0b243f --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_28/data.json.error @@ -0,0 +1,5 @@ +[ + 280875, + 281075, + 289298 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_29/data.json b/retranslated_vi_pubmed old format/chunk_29/data.json new file mode 100644 index 0000000000000000000000000000000000000000..4cce15e2cb9852132da8e7620b15c0e10a13da4f --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_29/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:1708bf47291750fa7a19ea6e6256ffb6a212d88293ce4076cf8093b8d2d8f231 +size 33971819 diff --git a/retranslated_vi_pubmed old 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--git a/retranslated_vi_pubmed old format/chunk_32/data.json b/retranslated_vi_pubmed old format/chunk_32/data.json new file mode 100644 index 0000000000000000000000000000000000000000..46b8b35ee7aeca57aa8b8540c9050c66901c609d --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_32/data.json @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:5b2ee6002c779b6cdaea08848fbbe940390cfc3fdbb27cb255bb90e993e5cac0 +size 24302869 diff --git a/retranslated_vi_pubmed old format/chunk_32/data.json.error b/retranslated_vi_pubmed old format/chunk_32/data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed old format/chunk_32/data.json.error @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed old format/chunk_33/data.json b/retranslated_vi_pubmed old format/chunk_33/data.json new file mode 100644 index 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index 0000000000000000000000000000000000000000..e182fbac940f28ba1b94b3ae49ba906e759cc2de --- /dev/null +++ b/retranslated_vi_pubmed/chunk_126/zbackup_data.json @@ -0,0 +1,77 @@ +[ + { + "idx": 1260000, + "en": "With the rapid expansion of impervious surfaces, urban waterlogging has become a typical \"urban disease\" in China, seriously hindering the sustainable development of cities. Therefore, reducing the impact of impervious surfaces on surface runoff is an effective approach to alleviate urban waterlogging. Presently, the development mode of many cities in China has shifted from an increase in urban scale to the improvement of urban quality through urban renewal, which is the current and future development path for most cities. Optimizing the design of impervious surfaces in urban renewal planning to reduce its impact on surface runoff is an important way to prevent and control urban waterlogging. The aim of this research is to construct an optimization model of impervious surface space layout under the framework of a geographic simulation technology-integrated ant colony optimization (ACO) and Soil Conservation Service curve number (SCS-CN) model (ACO-SCS) in a case study of Guangzhou in China. Urban runoff plots in the study area are divided according to the area of the urban planning unit. With the goal of minimizing the runoff coefficient, the optimal space layout of the impervious surfaces is obtained, which provides a technical method and reference for urban waterlogging prevention and control through urban renewal planning. The results reveal that the optimization of impervious surface space layout through ACO-SCS achieves a satisfactory effect with an average optimization rate of 9.52%, and a maximum optimization rate of 33.16%. The research also shows that the initial impervious surface layout is the key influencing factor in ACO-SCS. In the urban renewal planning stage, the space layout of the impervious surfaces with a high-low-high density discontinuous connection can be constructed by transforming medium-density impervious surfaces into low-density impervious surfaces to achieve the flat and long-type agglomeration of the low-density and high-density impervious surfaces, which can effectively reduce the influence of urban development on surface runoff. There is spatial heterogeneity of the optimal results in different urban runoff plots. Therefore, the policy of urban renewal planning for urban waterlogging prevention and control should be different. The optimized results of impervious surface space layout provide useful reference information for urban renewal planning.", + "gemini": "Với sự mở rộng nhanh chóng của các bề mặt không thấm nước, tình trạng ngập úng đô thị đã trở thành một \"bệnh đô thị\" điển hình ở Trung Quốc, cản trở nghiêm trọng đến sự phát triển bền vững của các đô thị. Do đó, việc giảm tác động của các bề mặt không thấm nước đối với dòng chảy bề mặt là một cách tiếp cận hiệu quả để giảm ngập úng đô thị. Hiện nay, mô hình phát triển của nhiều thành phố ở Trung Quốc đã chuyển từ tăng quy mô đô thị sang cải thiện chất lượng đô thị thông qua đổi mới đô thị, đây là con đường phát triển hiện tại và tương lai của hầu hết các thành phố. Tối ưu hóa thiết kế các bề mặt không thấm nước trong quy hoạch cải tạo đô thị để giảm tác động của chúng đối với dòng chảy bề mặt là một cách quan trọng để ngăn ngừa và kiểm soát ngập úng đô thị. Mục đích của nghiên cứu này là xây dựng một mô hình tối ưu hóa bố cục không gian bề mặt không thấm nước trong khuôn khổ của công nghệ mô phỏng địa lý tích hợp tối ưu hóa đàn kiến (ACO) và mô hình số đường cong dịch vụ bảo tồn đất (SCS-CN) (ACO-SCS) trong một nghiên cứu trường hợp ở Quảng Châu, Trung Quốc. Các ô dòng chảy đô thị trong khu vực nghiên cứu được phân chia theo diện tích của đơn vị quy hoạch đô thị. Với mục tiêu giảm thiểu hệ số dòng chảy, bố cục không gian tối ưu của các bề mặt không thấm nước được thu được, cung cấp phương pháp kỹ thuật và tài liệu tham khảo để phòng ngừa và kiểm soát ngập úng đô thị thông qua quy hoạch cải tạo đô thị. Kết quả cho thấy việc tối ưu hóa bố cục không gian bề mặt không thấm nước thông qua ACO-SCS đạt hiệu quả khả quan với tỷ lệ tối ưu hóa trung bình là 9,52% và tỷ lệ tối ưu hóa tối đa là 33,16%. Nghiên cứu cũng chỉ ra rằng bố cục bề mặt không thấm nước ban đầu là yếu tố ảnh hưởng chính trong ACO-SCS. Trong giai đoạn quy hoạch cải tạo đô thị, có thể xây dựng bố cục không gian của các bề mặt không thấm nước có kết nối không liên tục mật độ cao-thấp-cao bằng cách chuyển đổi các bề mặt không thấm nước mật độ trung bình thành các bề mặt không thấm nước mật độ thấp để đạt được sự kết tụ phẳng và dài của các bề mặt không thấm nước mật độ thấp và mật độ cao, có thể làm giảm hiệu quả ảnh hưởng của phát triển đô thị đến dòng chảy bề mặt. Có tính không đồng nhất về mặt không gian của các kết quả tối ưu ở các ô dòng chảy đô thị khác nhau. Do đó, chính sách quy hoạch cải tạo đô thị để phòng ngừa và kiểm soát ngập úng đô thị cần phải khác nhau. Các kết quả tối ưu hóa bố cục không gian bề mặt không thấm nước cung cấp thông tin tham khảo hữu ích cho quy hoạch cải tạo đô thị." + }, + { + "idx": 1260001, + "en": "Aphis gossypii Glover (Hemiptera: Aphididae) is a serious polyphagous agricultural pest worldwide. In the present study, we used eight microsatellite markers to investigate the genetic structure and diversity of A. gossypii populations in Korea. Samples were collected from 37 locations in Korea (18 populations in 2016, 14 populations in 2017, and five populations in 2018) from pepper plants. A. gossypii had low to moderate genetic diversity, and expected heterozygosity (HE) ranged from 0.354 to 0.719. A Mantel test of isolation by distance indicated no relationship between genetic structure and geographic distance among all populations (r2 = 0.0004, p = 0.370), suggesting high gene flow among populations in Korea. Populations of A. gossypii in Korea were divided into two distinct genetic clusters (ΔK = 2). In 2016 and 2017, the genetic clusters changed into opposite genetic structures within one year mostly in northwest and southeast parts of Korea. Possible relevance of study results was discussed. Chemical control, cyclical parthenogenesis, and immigrants from the exterior might have resulted in this low genetic diversity and opposite genetic clusters.", + "gemini": "Aphis gossypii Glover (Hemiptera: Aphididae) là một loài gây hại nông nghiệp đa thực nghiêm trọng trên toàn thế giới. Trong nghiên cứu hiện tại, chúng tôi đã sử dụng tám dấu hiệu vi vệ tinh để nghiên cứu cấu trúc và sự đa dạng di truyền của quần thể A. gossypii ở Hàn Quốc. Mẫu được thu thập từ 37 địa điểm ở Hàn Quốc (18 quần thể vào năm 2016, 14 quần thể vào năm 2017 và năm quần thể vào năm 2018) từ cây ớt. A. gossypii có sự đa dạng di truyền từ thấp đến trung bình và độ dị hợp tử mong đợi (HE) dao động từ 0,354 đến 0,719. Kiểm định Mantel về sự cô lập theo khoảng cách không chỉ ra mối quan hệ giữa cấu trúc di truyền và khoảng cách địa lý giữa tất cả các quần thể (r2 = 0,0004, p = 0,370), cho thấy dòng gen cao giữa các quần thể ở Hàn Quốc. Các quần thể A. gossypii ở Hàn Quốc được chia thành hai nhóm di truyền riêng biệt (ΔK = 2). Vào năm 2016 và 2017, các nhóm di truyền đã thay đổi thành các cấu trúc di truyền đối lập trong vòng một năm chủ yếu ở các vùng tây bắc và đông nam của Hàn Quốc. Tầm quan trọng có thể có của kết quả nghiên cứu đã được thảo luận. Kiểm soát hóa học, sinh sản đơn tính theo chu kỳ và những người nhập cư từ bên ngoài có thể dẫn đến sự đa dạng di truyền thấp và các nhóm di truyền đối lập này." + }, + { + "idx": 1260002, + "en": "Mutations of the Telomerase reverse transcriptase (TERT) gene promoter are recurrently found in follicular thyroid carcinoma (FTC) and follicular tumors of uncertain malignant potential (FT-UMP), but nearly never in follicular thyroid adenoma (FTA). We, therefore, believe these mutations could signify malignant potential. At our department, postoperative TERT promoter mutational testing of FT-UMPs was implemented in 2014, with a positive mutation screening leading to vigilant follow-up and sometimes adjuvant treatment. To date, we screened 51 FT-UMPs and compared outcomes to 40 minimally invasive FTCs (miFTCs) with known TERT genotypes. Eight FT-UMPs (16%) displayed TERT promoter mutations, of which four cases underwent a completion lobectomy at the discretion of the patient, and a single patient also opted in for radioiodine (RAI) treatment. Three mutation-positive patients developed distant metastases, registered in one patient receiving a completion lobectomy and in two patients with no additional treatment. Three out of four patients who received additional surgery, including the RAI-treated patient, are still without metastatic disease. We conclude that FT-UMPs with TERT promoter mutations harbor malignant potential and exhibit at least similar recurrence rates to TERT-promoter-mutated miFTCs. Mutational screening should constitute a cornerstone analysis in the histopathological work-up of FT-UMPs.", + "gemini": "Đột biến của gen tiền ung thư Telomerase (TERT) thường được tìm thấy trong ung thư biểu mô tuyến giáp nang (FTC) và các khối u nang có tiềm năng ác tính không chắc chắn (FT-UMP), nhưng hầu như không bao giờ có trong u tuyến giáp nang (FTA). Do đó, chúng tôi tin rằng những đột biến này có thể biểu thị tiềm năng ác tính. Tại khoa của chúng tôi, xét nghiệm đột biến tiền ung thư TERT sau phẫu thuật của FT-UMP đã được thực hiện vào năm 2014, với việc sàng lọc đột biến dương tính dẫn đến theo dõi cảnh giác và đôi khi là điều trị bổ sung. Đến nay, chúng tôi đã sàng lọc 51 FT-UMP và so sánh kết quả với 40 FTC xâm lấn tối thiểu (miFTC) có kiểu gen TERT đã biết. Tám FT-UMP (16%) biểu hiện đột biến tiền ung thư TERT, trong đó bốn trường hợp đã cắt bỏ thùy hoàn toàn theo quyết định của bệnh nhân và một bệnh nhân cũng lựa chọn điều trị iốt phóng xạ (RAI). Ba bệnh nhân có đột biến dương tính đã phát triển di căn xa, được ghi nhận ở một bệnh nhân cắt bỏ thùy hoàn toàn và ở hai bệnh nhân không có thêm điều trị. Ba trong số bốn bệnh nhân được phẫu thuật bổ sung, bao gồm cả bệnh nhân được điều trị bằng RAI, vẫn không bị di căn. Chúng tôi kết luận rằng FT-UMP có đột biến tiền ung thư TERT chứa tiềm năng ác tính và biểu hiện tỷ lệ tái phát ít nhất là tương tự như miFTC có đột biến tiền ung thư TERT. Sàng lọc đột biến nên cấu thành một phân tích nền tảng trong quá trình giải phẫu bệnh của FT-UMP." + }, + { + "idx": 1260003, + "en": "In this study, the shrinkage and creep of concrete containing graphene oxide (GO) nanosheets were experimentally and theoretically investigated. Experiments for the shrinkage and creep of concrete with 0.02% and 0.08% GO nanosheets by the weight of cement and common concrete were carried out. Subsequently, the influence of GO nanosheets on the shrinkage and creep of concrete was analyzed and discussed. A modified model was developed to accurately predict the shrinkage and creep of concrete containing GO nanosheets after models for predicting shrinkage and creep of common concrete were compared and the influential factors and application scope were determined. Results indicate that: (1) GO nanosheets can increase the shrinkage strain and reduce the creep coefficient of concrete, and (2) a modified ACI209 (92) model can accurately predict the shrinkage and creep of concrete containing GO nanosheets. Factors considering concrete strength can be introduced in the model to improve the model accuracy.", + "gemini": "Trong nghiên cứu này, sự co ngót và độ nhớt của bê tông chứa các tấm nano oxit graphene (GO) đã được nghiên cứu thực nghiệm và lý thuyết. Các thí nghiệm về độ co ngót và độ nhớt của bê tông với 0,02% và 0,08% tấm nano GO theo trọng lượng xi măng và bê tông thông thường đã được thực hiện. Sau đó, ảnh hưởng của các tấm nano GO đến độ co ngót và độ nhớt của bê tông đã được phân tích và thảo luận. Một mô hình đã được sửa đổi để dự đoán chính xác độ co ngót và độ nhớt của bê tông chứa các tấm nano GO sau khi các mô hình dự đoán độ co ngót và độ nhớt của bê tông thông thường được so sánh và các yếu tố có ảnh hưởng và phạm vi ứng dụng đã được xác định. Kết quả cho thấy: (1) Các tấm nano GO có thể làm tăng độ co ngót và giảm hệ số độ nhớt của bê tông và (2) mô hình ACI209 (92) đã sửa đổi có thể dự đoán chính xác độ co ngót và độ nhớt của bê tông chứa các tấm nano GO. Các yếu tố xem xét cường độ bê tông có thể được đưa vào mô hình để cải thiện độ chính xác của mô hình." + }, + { + "idx": 1260004, + "en": "Human infections with Brucella melitensis are occasionally reported in Sweden, despite the fact that the national flocks of sheep and goats are officially free from brucellosis. The aim of our study was to analyze 103 isolates of B. melitensis collected from patients in Sweden between 1994 and 2016 and determine their putative geographic origin using whole genome sequencing (WGS)-based tools. The majority of the strains were assigned to East Mediterranean and African lineages. Both in silico Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) and core genome Multilocus Sequence Typing (cgMLST) analyses identified countries of the Middle East as the most probable source of origin of the majority of the strains. Isolates collected from patients with travel history to Iraq or Syria were often associated with genotypes from Turkey, as the cgMLST profiles from these countries clustered together. Sixty strains were located within a distance of 20 core genes to related genotypes from the publicly available database, and for eighteen isolates, the closest genotype was different by more than 50 loci. Our study showed that WGS based tools are effective in tracing back the geographic origin of infection of patients with unknown travel status, provided that public sequences from the location of the source are available.", + "gemini": "Nhiễm trùng ở người do Brucella melitensis đôi khi được báo cáo ở Thụy Điển, mặc dù thực tế là các đàn cừu và dê quốc gia không có bệnh brucella. Mục đích của nghiên cứu của chúng tôi là phân tích 103 phân lập B. melitensis thu thập từ bệnh nhân ở Thụy Điển từ năm 1994 đến năm 2016 và xác định nguồn gốc địa lý có thể của chúng bằng cách sử dụng các công cụ dựa trên giải trình tự toàn bộ hệ gen (WGS). Phần lớn các chủng được phân loại vào các dòng Đông Địa Trung Hải và Châu Phi. Cả phân tích VNTR (Số lượng lặp lại tándem biến đổi) nhiều vị trí trong silico (MLVA) và phân tích gõ trình tự nhiều locus của bộ gen lõi (cgMLST) đều xác định các quốc gia Trung Đông là nguồn gốc có khả năng nhất của phần lớn các chủng. Các phân lập thu thập được từ những bệnh nhân có tiền sử đi du lịch đến Iraq hoặc Syria thường có liên quan đến kiểu gen từ Thổ Nhĩ Kỳ, vì các hồ sơ cgMLST từ các quốc gia này tập hợp lại với nhau. Sáu mươi chủng nằm trong khoảng cách 20 gen lõi so với các kiểu gen liên quan từ cơ sở dữ liệu công khai và đối với mười tám phân lập, kiểu gen gần nhất khác nhau ở hơn 50 locus. Nghiên cứu của chúng tôi cho thấy các công cụ dựa trên WGS có hiệu quả trong việc truy tìm nguồn gốc địa lý của bệnh nhiễm trùng ở những bệnh nhân có tình trạng đi lại không rõ, miễn là có các trình tự công khai từ vị trí của nguồn." + }, + { + "idx": 1260005, + "en": "Balancing the accuracy and speed of three-dimensional (3D) surface measurement of objects is crucial in many important applications. In this paper, we present a wrapped phase and pseudorandom image method and develop an experimental system aiming to avoid the process of phase unwrapping. Our approach can reduce the length of image sequences and improve the speed of pattern projection and image acquisition and can be used as a good candidate for high-speed 3D measurement. The most critical step in our new methodology is using the wrapped phase and the epipolar constraint between one camera and a projector, which can obtain several candidate 3D points within the measurement volume (MV). The false points from the obtained candidate 3D points can be eliminated by the pseudorandom images. A systematic accuracy with MV better than 0.01 mm is achievable. 3D human body measurement results are given to confirm the fast speed of image acquisition capability.", + "gemini": "Việc cân bằng độ chính xác và tốc độ đo bề mặt ba chiều (3D) của vật thể là rất quan trọng trong nhiều ứng dụng quan trọng. Trong bài báo này, chúng tôi trình bày một phương pháp hình ảnh pha được bọc và giả ngẫu nhiên và phát triển một hệ thống thực nghiệm nhằm tránh quá trình mở pha. Phương pháp tiếp cận của chúng tôi có thể làm giảm độ dài của chuỗi hình ảnh và cải thiện tốc độ chiếu mẫu và thu nhận hình ảnh và có thể được sử dụng như một ứng cử viên tốt cho phép đo 3D tốc độ cao. Bước quan trọng nhất trong phương pháp mới của chúng tôi là sử dụng pha được bọc và ràng buộc cực giữa một máy ảnh và một máy chiếu, có thể thu được một số điểm 3D ứng viên trong thể tích đo (MV). Các điểm giả từ các điểm 3D ứng viên thu được có thể bị loại bỏ bởi các hình ảnh giả ngẫu nhiên. Độ chính xác có hệ thống với MV tốt hơn 0,01 mm có thể đạt được. Kết quả đo cơ thể người 3D được đưa ra để xác nhận tốc độ thu nhận hình ảnh nhanh." + }, + { + "idx": 1260006, + "en": "The aims of this study were to determine Cu, Mn, Se, and Zn content in wild mushrooms collected from unpolluted areas of the eastern Green Lungs of Poland (GLP) territory, to compare them to some popular species of cultivated mushrooms, evaluate mushroom contribution to the daily intake of the studied bioelements, and to determine their possible toxic effect resulting from potentially excessive mushroom consumption from areas recognized as ecologically uncontaminated. Bioelements were determined in 21 species of edible mushrooms: eighteen species of wild mushrooms and three species of popular cultivated mushrooms. The mean Cu, Mn, Se, and Zn content (in µg/g, dry mass DM) ranged from 10.6-123.1, 12.2-41, 0.13-13.3, and 68.3-184, respectively. A comparison with recommended dietary allowance (RDA) for Cu, Se, and Zn as well as adequate intake (AI) for Mn demonstrated that a 100 g fresh mass (FM) portion of mushroom species with the highest content of a given element can meet the demand for Cu, Mn, Se and Zn at 203%, 14-17%, 211%, and 16-22%, respectively. A comparison of the content of the examined bioelements contained in one portion of mushrooms (100 g FM) against the toxicological intake limits for different chemical elements with the provisional maximum tolerable daily intake (PMTDI) and upper intake level (UL) showed no risk of toxicity for the evaluated mushroom species.", + "gemini": "Mục đích của nghiên cứu này là xác định hàm lượng Cu, Mn, Se và Zn trong nấm hoang thu thập từ các khu vực không bị ô nhiễm của lãnh thổ Phổi xanh phía đông Ba Lan (GLP), để so sánh chúng với một số loài nấm được nuôi trồng phổ biến, đánh giá sự đóng góp của nấm vào lượng hấp thụ sinh học hàng ngày các nguyên tố sinh học đã nghiên cứu và xác định tác dụng độc hại có thể có của chúng do tiêu thụ quá nhiều nấm từ các khu vực được công nhận là không bị ô nhiễm về mặt sinh thái. Các nguyên tố sinh học đã được xác định trong 21 loài nấm ăn được: mười tám loài nấm hoang và ba loài nấm được nuôi trồng phổ biến. Hàm lượng Cu, Mn, Se và Zn trung bình (tính theo µg/g, khối lượng khô DM) dao động từ 10,6-123,1, 12,2-41, 0,13-13,3 và 68,3-184. Khi so sánh với chế độ ăn uống khuyến nghị (RDA) đối với Cu, Se và Zn cũng như lượng hấp thụ đầy đủ (AI) đối với Mn, kết quả cho thấy một khẩu phần 100 g khối lượng tươi (FM) của loài nấm có hàm lượng cao nhất của một nguyên tố nhất định có thể đáp ứng nhu cầu về Cu, Mn, Se và Zn ở mức 203%, 14-17%, 211% và 16-22%. Khi so sánh hàm lượng các nguyên tố sinh học đã kiểm tra có trong một khẩu phần nấm (100 g FM) với giới hạn hấp thụ độc tính đối với các nguyên tố hóa học khác nhau với lượng hấp thụ tối đa tạm thời có thể dung nạp hàng ngày (PMTDI) và mức hấp thụ trên (UL) cho thấy không có nguy cơ độc tính đối với các loài nấm đã đánh giá." + }, + { + "idx": 1260007, + "en": "Passengers travelling on the London underground tubes currently have no means of knowing their whereabouts between stations. The challenge for providing such service is that the London underground tunnels have no GPS, Wi-Fi, Bluetooth, or any kind of terrestrial signals to leverage. This paper presents a novel yet practical idea to track passengers in realtime using the smartphone accelerometer and a training database of the entire London underground network. Our rationales are that London tubes are self-driving transports with predictable accelerations, decelerations, and travelling time and that they always travel on the same fixed rail lines between stations with distinctive bumps and vibrations, which permit us to generate an accelerometer map of the tubes' movements on each line. Given the passenger's accelerometer data, we identify in realtime what line they are travelling on and what station they depart from, using a pattern-matching algorithm, with an accuracy of up to about 90% when the sampling length is equivalent to at least 3 station stops. We incorporate Principal Component Analysis to perform inertial tracking of passengers' positions along the line when trains break away from scheduled movements during rush hours. Our proposal was painstakingly assessed on the entire London underground, covering approximately 940 km of travelling distance, spanning across 381 stations on 11 different lines.", + "gemini": "Hành khách đi trên tàu điện ngầm London hiện không có cách nào để biết vị trí của mình giữa các ga. Thách thức trong việc cung cấp dịch vụ như vậy là các đường hầm tàu điện ngầm London không có GPS, Wi-Fi, Bluetooth hoặc bất kỳ loại tín hiệu mặt đất nào để tận dụng. Bài báo này trình bày một ý tưởng mới nhưng thiết thực để theo dõi hành khách theo thời gian thực bằng cách sử dụng gia tốc kế điện thoại thông minh và cơ sở dữ liệu đào tạo của toàn bộ mạng lưới tàu điện ngầm London. Cơ sở lý luận của chúng tôi là các tuyến tàu điện ngầm London là phương tiện tự lái với khả năng tăng tốc, giảm tốc và thời gian di chuyển có thể dự đoán được và chúng luôn đi trên cùng một tuyến đường ray cố định giữa các ga với những va chạm và rung động đặc biệt, cho phép chúng tôi tạo ra một bản đồ gia tốc về chuyển động của các tuyến tàu trên mỗi tuyến. Với dữ liệu gia tốc kế của hành khách, chúng tôi xác định theo thời gian thực tuyến nào họ đang đi và họ khởi hành từ ga nào, bằng cách sử dụng thuật toán khớp mẫu, với độ chính xác lên đến khoảng 90% khi độ dài lấy mẫu tương đương với ít nhất 3 điểm dừng của ga. Chúng tôi kết hợp Phân tích thành phần chính để thực hiện theo dõi quán tính vị trí của hành khách dọc theo tuyến khi tàu hỏa thoát khỏi các chuyển động theo lịch trình trong giờ cao điểm. Đề xuất của chúng tôi đã được đánh giá cẩn thận trên toàn bộ tàu điện ngầm London, bao phủ khoảng 940 km khoảng cách di chuyển, trải dài trên 381 ga trên 11 tuyến khác nhau." + }, + { + "idx": 1260008, + "en": "Brain health refers to the preservation of brain integrity and function optimized for an individual's biological age. Several studies have demonstrated that our lifestyles habits impact our brain health and our cognitive and mental wellbeing. Monitoring such lifestyles is thus critical and mobile technologies are essential to enable such a goal. Three databases were selected to carry out the search. Then, a PRISMA and PICOTS based criteria for a more detailed review on the basis of monitoring lifestyle aspects were used to filter the publications. We identified 133 publications after removing duplicates. Fifteen were finally selected from our criteria. Many studies still use questionnaires as the only tool for monitoring and do not apply advanced analytic or AI approaches to fine-tune results. We anticipate a transformative boom in the near future developing and implementing solutions that are able to integrate, in a flexible and adaptable way, data from technologies and devices that users might already use. This will enable continuous monitoring of objective data to guide the personalized definition of lifestyle goals and data-driven coaching to offer the necessary support to ensure adherence and satisfaction.", + "gemini": "Sức khỏe não bộ đề cập đến việc bảo tồn tính toàn vẹn và chức năng của não được tối ưu hóa cho độ tuổi sinh học của một cá nhân. Một số nghiên cứu đã chứng minh rằng thói quen lối sống của chúng ta ảnh hưởng đến sức khỏe não bộ và sức khỏe nhận thức và tinh thần của chúng ta. Do đó, việc theo dõi các lối sống như vậy là rất quan trọng và các công nghệ di động rất cần thiết để đạt được mục tiêu đó. Ba cơ sở dữ liệu đã được chọn để thực hiện tìm kiếm. Sau đó, một tiêu chí dựa trên PRISMA và PICOTS để đánh giá chi tiết hơn về cơ sở theo dõi các khía cạnh lối sống đã được sử dụng để lọc các ấn phẩm. Chúng tôi đã xác định được 133 ấn phẩm sau khi loại bỏ các bản trùng lặp. Cuối cùng, mười lăm ấn phẩm đã được chọn từ các tiêu chí của chúng tôi. Nhiều nghiên cứu vẫn sử dụng bảng câu hỏi làm công cụ duy nhất để theo dõi và không áp dụng các phương pháp phân tích nâng cao hoặc AI để tinh chỉnh kết quả. Chúng tôi dự đoán một sự bùng nổ mang tính biến đổi trong tương lai gần, phát triển và triển khai các giải pháp có khả năng tích hợp, theo cách linh hoạt và có thể thích ứng, dữ liệu từ các công nghệ và thiết bị mà người dùng có thể đã sử dụng. Điều này sẽ cho phép theo dõi liên tục dữ liệu khách quan để hướng dẫn định nghĩa được cá nhân hóa về các mục tiêu lối sống và hướng dẫn dựa trên dữ liệu để đưa ra sự hỗ trợ cần thiết nhằm đảm bảo tính tuân thủ và sự hài lòng." + }, + { + "idx": 1260009, + "en": "We present a model that estimates the spectral phytoplankton absorption coefficient ( a p h ( λ ) ) of four phytoplankton groups (picophytoplankton, nanophytoplankton, dinoflagellates, and diatoms) as a function of the total chlorophyll-a concentration (C) and sea surface temperature (SST). Concurrent data on a p h ( λ ) (at 12 visible wavelengths), C and SST, from the surface layer (<20 m depth) of the North Atlantic Ocean, were partitioned into training and independent validation data, the validation data being matched with satellite ocean-colour observations. Model parameters (the chlorophyll-specific phytoplankton absorption coefficients of the four groups) were tuned using the training data and found to compare favourably (in magnitude and shape) with results of earlier studies. Using the independent validation data, the new model was found to retrieve total a p h ( λ ) with a similar performance to two earlier models, using either in situ or satellite data as input. Although more complex, the new model has the advantage of being able to determine a p h ( λ ) for four phytoplankton groups and of incorporating the influence of SST on the composition of the four groups. We integrate the new four-population absorption model into a simple model of ocean colour, to illustrate the influence of changes in SST on phytoplankton community structure, and consequently, the blue-to-green ratio of remote-sensing reflectance. We also present a method of propagating error through the model and illustrate the technique by mapping errors in group-specific a p h ( λ ) using a satellite image. We envisage the model will be useful for ecosystem model validation and assimilation exercises and for investigating the influence of temperature change on ocean colour.", + "gemini": "Chúng tôi trình bày một mô hình ước tính hệ số hấp thụ quang phổ của thực vật phù du (a p h (λ)) của bốn nhóm thực vật phù du (thực vật phù du nhỏ, thực vật phù du nano, tảo giáp và tảo cát) như một hàm của tổng nồng độ chlorophyll-a (C) và nhiệt độ bề mặt biển (SST). Dữ liệu đồng thời về a p h (λ) (ở 12 bước sóng khả kiến), C và SST, từ lớp bề mặt (<20 m độ sâu) của Bắc Đại Tây Dương, được phân thành dữ liệu đào tạo và dữ liệu xác thực độc lập, dữ liệu xác thực được khớp với các quan sát màu đại dương vệ tinh. Các tham số mô hình (các hệ số hấp thụ quang phổ của thực vật phù du đặc hiệu của chlorophyll của bốn nhóm) được điều chỉnh bằng cách sử dụng dữ liệu đào tạo và được phát hiện là so sánh thuận lợi (về độ lớn và hình dạng) với kết quả của các nghiên cứu trước đó. Sử dụng dữ liệu xác thực độc lập, mô hình mới được phát hiện để thu hồi tổng a p h (λ) với hiệu suất tương tự như hai mô hình trước đó, sử dụng dữ liệu tại chỗ hoặc vệ tinh làm đầu vào. Mặc dù phức tạp hơn, mô hình mới có ưu điểm là có thể xác định a p h (λ) cho bốn nhóm thực vật phù du và kết hợp ảnh hưởng của SST vào thành phần của bốn nhóm. Chúng tôi tích hợp mô hình hấp thụ bốn quần thể mới vào một mô hình đơn giản về màu đại dương, để minh họa ảnh hưởng của những thay đổi trong SST đối với cấu trúc quần xã thực vật phù du và do đó, tỷ lệ phản xạ cảm biến từ xa từ xanh lam sang xanh lục. Chúng tôi cũng trình bày một phương pháp lan truyền lỗi thông qua mô hình và minh họa kỹ thuật bằng cách lập bản đồ lỗi trong a p h (λ) theo nhóm cụ thể bằng cách sử dụng hình ảnh vệ tinh. Chúng tôi hình dung rằng mô hình sẽ hữu ích cho các bài tập xác thực và đồng hóa mô hình hệ sinh thái và để điều tra ảnh hưởng của sự thay đổi nhiệt độ đối với màu đại dương." + }, + { + "idx": 1260010, + "en": "Obsessive‒compulsive disorder (OCD), and especially contamination obsessions and washing compulsions, has been related to disgust. However, when its cardiovascular correlates have been studied, contradictory results have been found, including heart rate accelerations and decelerations. The aim of this study is to analyze emotional, cognitive, and cardiovascular responses in nonclinical (control) and subclinical participants with obsessive‒compulsive contamination/washing symptoms when confronted with a disgusting stimulus. Twenty-seven participants (14 subclinical OCD) completed a behavioral avoidance task with a contamination-based stimulus while their heart rate and subjective variables were measured. Results showed heart rate reductions in both samples, whereas subjective measures reflected higher disgust, anxiety, dirtiness, and emotional valence in the subclinical sample. However, at the same time, the sense of dominance was lower in the control group. In conclusion, our results support a heart rate deceleration during exposure to a disgusting stimulus dissociated from the subjective experience.", + "gemini": "Rối loạn ám ảnh cưỡng chế (OCD), và đặc biệt là ám ảnh về sự ô nhiễm và hành vi rửa trôi, có liên quan đến sự ghê tởm. Tuy nhiên, khi các tương quan về tim mạch của nó được nghiên cứu, người ta đã tìm thấy những kết quả trái ngược nhau, bao gồm cả sự tăng và giảm nhịp tim. Mục đích của nghiên cứu này là phân tích các phản ứng về cảm xúc, nhận thức và tim mạch ở những người không mắc bệnh (nhóm chứng) và những người mắc các triệu chứng ám ảnh cưỡng chế về ô nhiễm/rửa trôi ở mức độ cận lâm sàng khi đối mặt với một kích thích gây ghê tởm. Hai mươi bảy người tham gia (14 người mắc OCD cận lâm sàng) đã hoàn thành nhiệm vụ tránh hành vi với một kích thích dựa trên sự ô nhiễm trong khi nhịp tim và các biến số chủ quan của họ được đo. Kết quả cho thấy nhịp tim giảm ở cả hai nhóm mẫu, trong khi các biện pháp chủ quan phản ánh mức độ ghê tởm, lo lắng, bẩn thỉu và giá trị cảm xúc cao hơn ở nhóm cận lâm sàng. Tuy nhiên, đồng thời, cảm giác thống trị thấp hơn ở nhóm chứng. Tóm lại, kết quả của chúng tôi ủng hộ sự giảm nhịp tim trong quá trình tiếp xúc với một kích thích gây ghê tởm tách biệt khỏi trải nghiệm chủ quan." + }, + { + "idx": 1260011, + "en": "Corynespora leaf spot caused by Corynespora cassiicola is one of the major diseases in cucumber (Cucumis sativus L.). However, the resistance mechanisms and signals of cucumber to C. cassiicola are unclear. Here, we report that the mildew resistance locus O (MLO) genes, CsMLO1 and CsMLO2, are both negative modulators of the cucumber defense response to C. cassiicola. Subcellular localization analysis showed that CsMLO1 and CsMLO2 are localized in the plasma membrane. Expression analysis indicated that the transcript levels of CsMLO1 and CsMLO2 are linked to the defense response to C. cassiicola. Transient overexpression of either CsMLO1 or CsMLO2 in cucumber cotyledons reduced resistance to C. cassiicola, whereas silencing of either CsMLO1 or CsMLO2 enhanced resistance to C. cassiicola. The relationships of pathogenesis-related proteins, reactive oxygen species (ROS)-associated genes, and abscisic acid (ABA)-related genes to the overexpression and silencing of CsMLO1/CsMLO2 in non-infested cucumber plants were investigated. The results indicated that CsMLO1 mediated resistance against C. cassiicola by regulating the expression of pathogenesis-related proteins and ROS-associated genes, as well as through ABA signaling pathway-associated genes. The CsMLO2-mediated resistance against C. cassiicola primarily involves regulation of the expression of pathogenesis-related proteins. Our findings will guide strategies to enhance the resistance of cucumber to corynespora leaf spot.", + "gemini": "Bệnh đốm lá Corynespora do nấm Corynespora cassiicola gây ra là một trong những bệnh chính ở dưa chuột (Cucumis sativus L.). Tuy nhiên, cơ chế kháng bệnh và tín hiệu của dưa chuột đối với C. cassiicola vẫn chưa rõ ràng. Ở đây, chúng tôi báo cáo rằng các gen MLO (mildew resistance locus O), CsMLO1 và CsMLO2, đều là những chất điều hòa tiêu cực của phản ứng phòng vệ của dưa chuột đối với C. cassiicola. Phân tích định vị dưới tế bào cho thấy CsMLO1 và CsMLO2 được định vị ở màng tế bào chất. Phân tích biểu hiện cho thấy mức phiên mã của CsMLO1 và CsMLO2 có liên quan đến phản ứng phòng vệ đối với C. cassiicola. Sự biểu hiện quá mức tạm thời của CsMLO1 hoặc CsMLO2 ở lá mầm dưa chuột làm giảm khả năng kháng bệnh đối với C. cassiicola, trong khi việc làm im lặng CsMLO1 hoặc CsMLO2 đều làm tăng khả năng kháng bệnh đối với C. cassiicola. Mối quan hệ của các protein liên quan đến sinh bệnh, các gen liên quan đến các loài oxy phản ứng (ROS) và các gen liên quan đến axit abscisic (ABA) với sự biểu hiện quá mức và im lặng của CsMLO1/CsMLO2 ở cây dưa chuột không bị nhiễm đã được nghiên cứu. Kết quả chỉ ra rằng CsMLO1 đã trung gian khả năng kháng bệnh đối với C. cassiicola bằng cách điều chỉnh biểu hiện của các protein liên quan đến sinh bệnh và các gen liên quan đến ROS, cũng như thông qua các gen liên quan đến con đường truyền tín hiệu ABA. Khả năng kháng bệnh do CsMLO2 trung gian đối với C. cassiicola chủ yếu liên quan đến việc điều chỉnh biểu hiện của các protein liên quan đến sinh bệnh. Những phát hiện của chúng tôi sẽ hướng dẫn các chiến lược để tăng cường khả năng kháng bệnh của dưa chuột đối với bệnh đốm lá corynespora." + }, + { + "idx": 1260015, + "en": "Chronic obstructive pulmonary disease (COPD) accounts for the largest proportion of respiratory deaths worldwide and was historically the leading indication for lung transplantation. The success of lung transplantation procedures is measured as survival benefit, calculated as survival with transplantation minus predicted survival without transplantation. In chronic obstructive pulmonary disease, it is difficult to show a clear and consistent survival benefit. Increasing knowledge of the risk factors, phenotypical heterogeneity, systemic manifestations, and their management helps improve our ability to select candidates and list those that will benefit the most from the procedure.", + "gemini": "Bệnh phổi tắc nghẽn mạn tính (COPD) chiếm tỷ lệ tử vong do hô hấp lớn nhất trên toàn thế giới và trong lịch sử là chỉ định hàng đầu cho ghép phổi. Sự thành công của các thủ thuật ghép phổi được đo bằng lợi ích về sự sống còn, được tính bằng sự sống còn sau ghép trừ đi sự sống còn dự đoán mà không ghép. Trong bệnh phổi tắc nghẽn mạn tính, rất khó để chứng minh lợi ích rõ ràng và nhất quán về sự sống còn. Việc tăng cường hiểu biết về các yếu tố nguy cơ, tính không đồng nhất về kiểu hình, biểu hiện toàn thân và cách quản lý của chúng giúp cải thiện khả năng lựa chọn ứng viên và liệt kê những người sẽ được hưởng lợi nhiều nhất từ thủ thuật này." + }, + { + "idx": 1260016, + "en": "A heatsink is a large experimental device which is used to simulate the outer space environment. In this paper, a Raman-based distributed temperature sensor was used for real-time and continuous heatsink temperature monitoring, and a special Raman-based distributed temperature sensing method and system have been proposed. This method takes advantage of three calibration parameters ( Δ α , γ , C ) to calculate the temperature. These three parameters are related to the attenuation of the optical fiber, the Raman translation, and the difference of optoelectronic conversion, respectively. Optical time domain reflectometry was used to calculate the location. A series of heatsink temperature measurement experiments were performed in a vacuum and -173 °C environment. When the temperature dropped to -100 °C, the parameter Δ α was found to vary. A method was proposed to recalculate Δ α and modify the traditional Raman fiber temperature equation. The results of the experiments confirmed the validity of this modified Raman fiber temperature equation. Based on this modified equation, the temperature field in the heatsink was calculated. The Raman-based distributed temperature sensor has potential applications in temperature measurement and judging the occurrence of faults in space exploration.", + "gemini": "Tản nhiệt là một thiết bị thử nghiệm lớn được sử dụng để mô phỏng môi trường không gian bên ngoài. Trong bài báo này, một cảm biến nhiệt độ phân tán dựa trên Raman đã được sử dụng để theo dõi nhiệt độ tản nhiệt liên tục và thời gian thực, và một phương pháp và hệ thống cảm biến nhiệt độ phân tán dựa trên Raman đặc biệt đã được đề xuất. Phương pháp này tận dụng ba tham số hiệu chuẩn ( Δ α , γ , C ) để tính nhiệt độ. Ba tham số này lần lượt liên quan đến sự suy giảm của sợi quang, sự dịch chuyển Raman và sự khác biệt về chuyển đổi quang điện. Phản xạ kế miền thời gian quang học được sử dụng để tính vị trí. Một loạt các thí nghiệm đo nhiệt độ tản nhiệt đã được thực hiện trong môi trường chân không và -173 °C. Khi nhiệt độ giảm xuống -100 °C, người ta thấy tham số Δ α thay đổi. Một phương pháp đã được đề xuất để tính toán lại Δ α và sửa đổi phương trình nhiệt độ sợi Raman truyền thống. Kết quả của các thí nghiệm đã xác nhận tính hợp lệ của phương trình nhiệt độ sợi Raman đã sửa đổi này. Dựa trên phương trình đã sửa đổi này, trường nhiệt độ trong tản nhiệt đã được tính toán. Cảm biến nhiệt độ phân tán dựa trên Raman có các ứng dụng tiềm năng trong việc đo nhiệt độ và đánh giá sự xuất hiện của các lỗi trong quá trình thám hiểm không gian." + }, + { + "idx": 1260017, + "en": "Atherosclerosis is a major cardiovascular disease worldwide, that could benefit from innovative nanomedicine imaging tools and treatments. In this perspective, we here studied, by fluorescence imaging in ApoE-/- mice, the biodistribution of non-functionalized and RXP470.1-targeted nanostructured lipid carriers (NLC) loaded with DiD dye. RXP470.1 specifically binds to MMP12, a metalloprotease that is over-expressed by macrophages residing in atherosclerotic plaques. Physico-chemical characterizations showed that RXP-NLC (about 105 RXP470.1 moieties/particle) displayed similar features as non-functionalized NLC in terms of particle diameter (about 60-65 nm), surface charge (about -5 - -10 mV), and colloidal stability. In vitro inhibition assays demonstrated that RXP-NLC conserved a selectivity and affinity profile, which favored MMP-12. In vivo data indicated that NLC and RXP-NLC presented prolonged blood circulation and accumulation in atherosclerotic lesions in a few hours. Twenty-four hours after injection, particle uptake in atherosclerotic plaques of the brachiocephalic artery was similar for both nanoparticles, as assessed by ex vivo imaging. This suggests that the RXP470.1 coating did not significantly induce an active targeting of the nanoparticles within the plaques. Overall, NLCs appeared to be very promising nanovectors to efficiently and specifically deliver imaging agents or drugs in atherosclerotic lesions, opening avenues for new nanomedicine strategies for cardiovascular diseases.", + "gemini": "Xơ vữa động mạch là một bệnh tim mạch chính trên toàn thế giới, có thể được hưởng lợi từ các công cụ và phương pháp điều trị hình ảnh nano y học sáng tạo. Theo quan điểm này, chúng tôi đã nghiên cứu ở đây, bằng hình ảnh huỳnh quang ở chuột ApoE-/- , sự phân bố sinh học của các chất mang lipid có cấu trúc nano không chức năng và nhắm mục tiêu RXP470.1 (NLC) được tải với thuốc nhuộm DiD. RXP470.1 liên kết đặc hiệu với MMP12, một metalloprotease được biểu hiện quá mức bởi các đại thực bào cư trú trong các mảng xơ vữa động mạch. Các đặc điểm vật lý-hóa học cho thấy RXP-NLC (khoảng 105 nhóm RXP470.1/hạt) biểu hiện các đặc điểm tương tự như NLC không chức năng về đường kính hạt (khoảng 60-65 nm), điện tích bề mặt (khoảng -5 - -10 mV) và độ ổn định keo. Các xét nghiệm ức chế trong ống nghiệm chứng minh rằng RXP-NLC bảo toàn cấu hình chọn lọc và ái lực, có lợi cho MMP-12. Dữ liệu trong ống nghiệm chỉ ra rằng NLC và RXP-NLC có sự lưu thông máu kéo dài và tích tụ trong các tổn thương xơ vữa động mạch sau vài giờ. Hai mươi bốn giờ sau khi tiêm, sự hấp thụ hạt trong các mảng xơ vữa động mạch của động mạch não chủ là tương tự đối với cả hai loại hạt nano, như được đánh giá bằng hình ảnh ngoại sinh. Điều này cho thấy lớp phủ RXP470.1 không tạo ra đáng kể sự nhắm mục tiêu tích cực của các hạt nano trong các mảng bám. Nhìn chung, NLC dường như là các hạt nano rất hứa hẹn để đưa hiệu quả và đặc hiệu các tác nhân tạo ảnh hoặc thuốc vào các tổn thương xơ vữa động mạch, mở ra con đường cho các chiến lược nano y học mới cho các bệnh tim mạch." + } +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_126/zbackup_data.json.error b/retranslated_vi_pubmed/chunk_126/zbackup_data.json.error new file mode 100644 index 0000000000000000000000000000000000000000..060db94102072458bd347ec080bbac28f998cc00 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_126/zbackup_data.json.error @@ -0,0 +1,11 @@ +[ + 1260012, + 1260013, + 1260014, + 1260018, + 1260019, + 1260020, + 1260021, + 1260022, + 1260023 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_127/completed.json b/retranslated_vi_pubmed/chunk_127/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..b47b35f78bd6d02a309880db1139abbab3651f1f --- /dev/null +++ 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newline at end of file diff --git a/retranslated_vi_pubmed/chunk_137/data.jsonl b/retranslated_vi_pubmed/chunk_137/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..807fb9145dafcb025ce8ebf2dc162dbe6175706f --- /dev/null +++ b/retranslated_vi_pubmed/chunk_137/data.jsonl @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:6fbad89d8afb1c8f60cbf6bdd0839042eed762e1450129e1b1688abd3f6b719a +size 28731275 diff --git a/retranslated_vi_pubmed/chunk_137/error.json b/retranslated_vi_pubmed/chunk_137/error.json new file mode 100644 index 0000000000000000000000000000000000000000..82fa7230ae012dccb46a5908cf22bedbb1707b12 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_137/error.json @@ -0,0 +1,11 @@ +[ + 1370008, + 1371149, + 1372787, + 1374079, + 1375609, + 1376061, + 1377025, + 1377333, + 1378253 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_138/completed.json b/retranslated_vi_pubmed/chunk_138/completed.json new file 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Advances in surgical techniques have helped improve outcomes of patients who are amenable to surgical resection. Sadly, surgery is amenable in only a small percentage of patients with the disease. Thus, there is a need to direct efforts at reducing the overall risk of pancreatic cancer or facilitating its early detection. The current review focused on collating all the literature published to date on pancreatic cancer in Australia with an aim to identify opportunities for intervention (at a state or national level) that are specific to Australia so as to help improve the outcome of patients with pancreatic cancer.", "gemini": "Ung thư tụy là một căn bệnh có liên quan đến tỷ lệ sống sót chung thấp. Những tiến bộ trong các kỹ thuật phẫu thuật đã giúp cải thiện kết quả cho những bệnh nhân có thể cắt bỏ bằng phẫu thuật. Đáng buồn thay, phẫu thuật chỉ có thể thực hiện ở một tỷ lệ phần trăm nhỏ bệnh nhân mắc bệnh này. Do đó, cần phải nỗ lực trực tiếp vào việc giảm nguy cơ mắc ung thư tụy nói chung hoặc tạo điều kiện phát hiện sớm bệnh ung thư tụy. Đánh giá hiện tại tập trung vào việc đối chiếu tất cả các tài liệu đã công bố cho đến nay về ung thư tụy ở Úc với mục đích xác định các cơ hội can thiệp (ở cấp tiểu bang hoặc quốc gia) cụ thể đối với Úc để giúp cải thiện kết quả cho bệnh nhân ung thư tụy."} +{"idx": 1870001, "en": "Concerns about the prospect of a global pandemic have been triggered many times during the last two decades. These have been realised through the current COVID-19 pandemic, due to a new coronavirus SARS-CoV2, which has impacted almost every country on Earth. Here, we show how considering the pandemic through the lenses of the evolutionary ecology of pathogens can help better understand the root causes and devise solutions to prevent the emergence of future pandemics. We call for better integration of these approaches into transdisciplinary research and invite scientists working on the evolutionary ecology of pathogens to contribute to a more \"solution-oriented\" agenda with practical applications, emulating similar movements in the field of economics in recent decades.", "gemini": "Mối quan ngại về viễn cảnh xảy ra đại dịch toàn cầu đã được nêu ra nhiều lần trong hai thập kỷ qua. Những mối quan ngại này đã được hiện thực hóa thông qua đại dịch COVID-19 hiện nay, do một loại coronavirus mới là SARS-CoV2 gây ra, đã tác động đến hầu hết mọi quốc gia trên Trái đất. Ở đây, chúng tôi chỉ ra cách xem xét đại dịch thông qua lăng kính của sinh thái học tiến hóa của các tác nhân gây bệnh có thể giúp hiểu rõ hơn về nguyên nhân gốc rễ và đưa ra các giải pháp để ngăn chặn sự xuất hiện của các đại dịch trong tương lai. Chúng tôi kêu gọi tích hợp tốt hơn các cách tiếp cận này vào nghiên cứu liên ngành và mời các nhà khoa học làm việc về sinh thái học tiến hóa của các tác nhân gây bệnh đóng góp vào một chương trình nghị sự \"hướng đến giải pháp\" hơn với các ứng dụng thực tế, noi theo các phong trào tương tự trong lĩnh vực kinh tế trong những thập kỷ gần đây."} +{"idx": 1870002, "en": "The development of new principles and techniques with high neuronal compatibility for quantitatively monitoring the dynamics of neurochemicals is essential for deciphering brain chemistry and function but remains a great challenge. We herein report a neuron-compatible method for in vivo neurochemical sensing by powering a single carbon fiber through spontaneous bipolar electrochemistry as a new sensing platform. By using ascorbic acid as a model target to prove the concept, we found that the single-carbon-fiber-powered microsensor exhibited a good response, high stability and, more importantly, excellent neuronal compatibility. The microsensor was also highly compatible with electrophysiological recording, thus enabling the synchronous recording of both chemical and electrical signals. The sensing principle could be developed for in vivo monitoring of various neurochemicals in the future by rationally designing and tuning the electrochemical reactions at the two poles of the carbon fiber.", "gemini": "Việc phát triển các nguyên lý và kỹ thuật mới có khả năng tương thích cao với tế bào thần kinh để theo dõi định lượng động lực của các chất hóa học thần kinh là rất cần thiết để giải mã hóa học và chức năng não nhưng vẫn là một thách thức lớn. Tại đây, chúng tôi báo cáo một phương pháp tương thích với tế bào thần kinh để cảm biến hóa học thần kinh trong cơ thể sống bằng cách cung cấp năng lượng cho một sợi carbon đơn lẻ thông qua điện hóa lưỡng cực tự phát như một nền tảng cảm biến mới. Bằng cách sử dụng axit ascorbic làm mục tiêu mô hình để chứng minh khái niệm, chúng tôi thấy rằng vi cảm biến được cung cấp năng lượng bằng sợi carbon đơn lẻ thể hiện phản ứng tốt, độ ổn định cao và quan trọng hơn là khả năng tương thích tuyệt vời với tế bào thần kinh. Vi cảm biến cũng rất tương thích với ghi điện sinh lý, do đó cho phép ghi đồng bộ cả tín hiệu hóa học và điện. Nguyên lý cảm biến có thể được phát triển để theo dõi trong cơ thể sống các chất hóa học thần kinh khác nhau trong tương lai bằng cách thiết kế và điều chỉnh hợp lý các phản ứng điện hóa ở hai cực của sợi carbon."} +{"idx": 1870003, "en": "Complement component C1q, a soluble defense collagen, is the recognition protein of the classical complement pathway. C1q is able to recognize and interact with multiple targets and, via the subsequent activation of its cognate serine proteases C1r and C1s, initiates the complement cascade. C1q is made up of six ABC heterotrimers each containing two different functional regions, an N-terminal collagen-like region (CLR) and a C-terminal globular region (GR). These heterotrimers assemble via their N-terminal regions, resulting in the characteristic 'bouquet-like' shape of C1q with an N-terminal bundle of collagen fibers with six diverging stems each exhibiting a C-terminal globular head. The GRs are responsible for the versatile recognition of multiple C1q targets, whereas the CLRs trigger immune response through interacting with several cellular or soluble partners. We report here the generation of the first recombinant form of human C1q without its recognition globular heads. The noncollagenous domain 2 (nc2) of type IX collagen has been substituted for the C1q GR in order to control the correct registering of the collagen triple helices of C1q chains A, B, and C. The resulting CLR_nc2 recombinant protein produced in stably transfected EXPI293 mammalian cells was correctly assembled and folded, as demonstrated by mass spectrometry, mass photometry, and electron microscopy experiments. Its interaction properties were investigated using surface plasmon resonance analysis with known CLR ligands: the tetramer of C1r and C1s dimers and MBL-associated protein MAp44. Comparison with the interaction properties of native serum-derived C1q and CLR revealed that recombinant CLR_nc2 retains C1q CLR functional properties.", "gemini": "Thành phần bổ thể C1q, một collagen phòng thủ hòa tan, là protein nhận dạng của con đường bổ thể cổ điển. C1q có thể nhận dạng và tương tác với nhiều mục tiêu và, thông qua quá trình hoạt hóa tiếp theo của các protease serine C1r và C1s đồng dạng của nó, khởi đầu quá trình bổ thể. C1q được tạo thành từ sáu dị hợp tử ABC, mỗi dị hợp tử chứa hai vùng chức năng khác nhau, một vùng giống collagen đầu N (CLR) và một vùng hình cầu đầu C (GR). Các dị hợp tử này lắp ráp thông qua các vùng đầu N của chúng, tạo ra hình dạng đặc trưng giống như bó hoa của C1q với một bó sợi collagen đầu N có sáu thân phân kỳ, mỗi thân đều biểu hiện một đầu hình cầu đầu C. Các GR chịu trách nhiệm cho sự nhận dạng linh hoạt của nhiều mục tiêu C1q, trong khi các CLR kích hoạt phản ứng miễn dịch thông qua tương tác với một số đối tác tế bào hoặc hòa tan. Chúng tôi báo cáo ở đây về việc tạo ra dạng tái tổ hợp đầu tiên của C1q của người mà không có các đầu hình cầu nhận dạng của nó. Miền không collagen 2 (nc2) của collagen loại IX đã được thay thế cho C1q GR để kiểm soát sự đăng ký chính xác của các chuỗi xoắn ba collagen của chuỗi C1q A, B và C. Protein tái tổ hợp CLR_nc2 thu được trong các tế bào động vật có vú EXPI293 được chuyển gen ổn định đã được lắp ráp và gấp chính xác, như được chứng minh bằng các thí nghiệm quang phổ khối, quang trắc khối và kính hiển vi điện tử. Các tính chất tương tác của nó đã được nghiên cứu bằng cách sử dụng phân tích cộng hưởng plasmon bề mặt với các phối tử CLR đã biết: tứ phân tử của các dị phân tử C1r và C1s và protein liên kết MBL MAp44. So sánh với các tính chất tương tác của C1q có nguồn gốc từ huyết thanh tự nhiên và CLR cho thấy CLR_nc2 tái tổ hợp vẫn giữ được các tính chất chức năng của C1q CLR."} +{"idx": 1870004, "en": "Vibrational polaritonic chemistry is emerging as an exciting new sub-field of chemistry, one in which strong interactions with optical cavity vacuum fields are another degree of freedom alongside temperature, solvent, catalyst, and so on to modify thermochemical reactivity. The field stands at a fascinating juncture with experimental works on a variety of organic reactions continuing to blossom, just as many theoretical works appear which diverge significantly in their predictions compared to experiments. The outlook for the field is no doubt an exciting one as it seeks to unify the observed novel optical cavity-induced chemical phenomena with satisfying accompanying physical theory. In this minireview we highlight experimental works on vibrational polaritonic chemistry that have appeared most recently, focusing on the chemistry of the rate-limiting steps to provide mechanistic insight. We hope this review will encourage synthetic chemists to enter the field and we discuss the opportunities and challenges that lie ahead as polaritonic chemistry grows into the future.", "gemini": "Hóa học phân cực dao động đang nổi lên như một lĩnh vực phụ mới thú vị của hóa học, trong đó tương tác mạnh với các trường chân không của khoang quang là một mức độ tự do khác cùng với nhiệt độ, dung môi, chất xúc tác, v.v. để sửa đổi phản ứng nhiệt hóa học. Lĩnh vực này đứng ở một ngã ba hấp dẫn với các công trình thực nghiệm về nhiều phản ứng hữu cơ tiếp tục phát triển, cũng như nhiều công trình lý thuyết xuất hiện có sự khác biệt đáng kể trong các dự đoán của họ so với các thí nghiệm. Triển vọng của lĩnh vực này chắc chắn là một lĩnh vực thú vị vì nó tìm cách thống nhất các hiện tượng hóa học mới lạ do khoang quang quan sát được với lý thuyết vật lý đi kèm thỏa đáng. Trong bài tổng quan nhỏ này, chúng tôi nêu bật các công trình thực nghiệm về hóa học phân cực dao động đã xuất hiện gần đây nhất, tập trung vào hóa học của các bước giới hạn tốc độ để cung cấp hiểu biết về cơ chế. Chúng tôi hy vọng bài đánh giá này sẽ khuyến khích các nhà hóa học tổng hợp tham gia vào lĩnh vực này và chúng tôi thảo luận về các cơ hội và thách thức phía trước khi hóa học phân cực phát triển trong tương lai."} +{"idx": 1870005, "en": "OBJECTIVES Problem bladder pain syndrome/interstitial cystitis (BPS/IC) is a heterogeneous disorder with variation in management worldwide. Phenotyping aims to personalize therapy and optimize outcomes. The most well-described phenotype is Hunner lesion disease (HLD). The prevalence of HLD and outcome of phenotype-directed management in the UK is not well-studied. We describe the management of a contemporary cohort of patients with BPS/IC in the UK. METHODS Retrospective analysis of all patients with BPS/IC from January 2015-November 2018. Outcomes of patients who underwent laser ablation to Hunner lesions were collected using the Global Response Assessment tool. RESULTS One hundred and sixty-three patients (mean age of 43 years [20-85]) were included. 78% were female and patients had experienced symptoms for an average 6 years (1-30) prior to specialist assessment. Eighty-three percent of patients had pelvic imaging (44% ultrasound, 42% magnetic resonance imaging and 14% computed tomography), and a relevant abnormality was found in five (4%). Twenty-two patients (14%) had HLD (International Society for the Study of BPS [ESSIC] 3), with a mean bladder capacity of 373 mL (175-650 mL); 77% were ESSIC C on histopathology. All patients with HLD underwent laser ablation, with 55% experiencing a moderate/marked improvement in symptoms, with a mean duration of effect of 10 months (3-36); 27% of patients had a repeat treatment. CONCLUSIONS The presence of HLD in patients with BPS/IC is not uncommon. Pelvic imaging rarely identifies any cause for pain and so cystoscopy under anesthesia is essential for accurate phenotyping. Phenotype-directed management with holmium laser ablation to Hunner lesions has good short-term efficacy in improving pain, but re-intervention is often required.", "gemini": "MỤC TIÊU Hội chứng đau bàng quang/viêm kẽ bàng quang (BPS/IC) là một rối loạn không đồng nhất với sự khác biệt về cách xử trí trên toàn thế giới. Phân loại kiểu hình nhằm mục đích cá nhân hóa liệu pháp và tối ưu hóa kết quả. Kiểu hình được mô tả tốt nhất là bệnh tổn thương Hunner (HLD). Tỷ lệ mắc bệnh HLD và kết quả của việc xử trí theo kiểu hình ở Vương quốc Anh chưa được nghiên cứu kỹ. Chúng tôi mô tả cách xử trí một nhóm bệnh nhân BPS/IC đương thời ở Vương quốc Anh. PHƯƠNG PHÁP Phân tích hồi cứu tất cả bệnh nhân mắc BPS/IC từ tháng 1 năm 2015 đến tháng 11 năm 2018. Kết quả của những bệnh nhân đã trải qua quá trình cắt bỏ bằng laser đối với các tổn thương Hunner đã được thu thập bằng công cụ Đánh giá phản ứng toàn cầu. KẾT QUẢ Một trăm sáu mươi ba bệnh nhân (tuổi trung bình 43 tuổi [20-85]) đã được đưa vào. 78% là nữ và bệnh nhân đã có các triệu chứng trung bình 6 năm (1-30) trước khi được chuyên gia đánh giá. 83% bệnh nhân đã chụp ảnh vùng chậu (44% siêu âm, 42% chụp cộng hưởng từ và 14% chụp cắt lớp vi tính) và một bất thường có liên quan đã được tìm thấy ở năm (4%). Hai mươi hai bệnh nhân (14%) bị HLD (Hiệp hội quốc tế nghiên cứu về BPS [ESSIC] 3), với dung tích bàng quang trung bình là 373 mL (175-650 mL); 77% là ESSIC C trên mô bệnh học. Tất cả bệnh nhân bị HLD đều được cắt bỏ bằng laser, 55% trong số họ có triệu chứng cải thiện ở mức độ trung bình/đáng kể, với thời gian có hiệu lực trung bình là 10 tháng (3-36); 27% bệnh nhân được điều trị lại. KẾT LUẬN Sự hiện diện của HLD ở những bệnh nhân mắc BPS/IC không phải là hiếm. Chụp hình ảnh vùng chậu hiếm khi xác định được bất kỳ nguyên nhân nào gây đau và do đó, nội soi bàng quang dưới gây mê là rất cần thiết để phân loại kiểu hình chính xác. Việc xử trí theo kiểu hình với cắt bỏ bằng laser holmium đối với các tổn thương Hunner có hiệu quả tốt trong thời gian ngắn để cải thiện cơn đau, nhưng thường phải can thiệp lại."} +{"idx": 1870006, "en": "Most microorganisms from deep terrestrial subsurface remain yet uncultured. Recent achievements in recovery of metagenome-assembled genomes (MAG) provide clues for improving cultivation via metabolic reconstructions and other genomic characteristics. Here we report the isolation in pure culture of a thermophilic spirochete with the use of MAGs binned from metagenomes of the deep (>2 km) aquifers broached by two artesian boreholes in Western Siberia. The organism constitutes a minor share in the aquifer microbial community and could not be cultivated by traditional techniques. The obtained two pure culture isolates along with three bacteria identified by MAGs represent a novel family-level lineage in the order Brevinematales. Based on genomic and phenotypic characteristics the novel spirochete is proposed to be classified as Longinema margulisiae gen. nov., sp. nov. within a novel family, Longinemaceae fam. nov. Both cultivated strains, NST and N5R, are anaerobic hemoorganoheterotrophes growing by fermentation of starch and a few sugars. They can form recalcitrant round bodies under unfavourable growth conditions, which survive up to 15 min at 95°C and can revert to the original helical cells. We suggest that the round bodies may facilitate global distribution of this lineage, detected from molecular signaturesand colonization of subsurface environments.", "gemini": "Hầu hết các vi sinh vật từ sâu dưới bề mặt đất vẫn chưa được nuôi cấy. Những thành tựu gần đây trong việc phục hồi các bộ gen lắp ráp từ metagenome (MAG) cung cấp manh mối để cải thiện việc nuôi cấy thông qua tái tạo chuyển hóa và các đặc điểm bộ gen khác. Ở đây chúng tôi báo cáo việc phân lập trong nuôi cấy thuần chủng một xoắn khuẩn ưa nhiệt bằng cách sử dụng MAG được phân loại từ metagenome của các tầng chứa nước sâu (>2 km) được khai thác bởi hai lỗ khoan artesian ở Tây Siberia. Sinh vật này chiếm một phần nhỏ trong cộng đồng vi sinh vật tầng chứa nước và không thể nuôi cấy bằng các kỹ thuật truyền thống. Hai phân lập nuôi cấy thuần chủng thu được cùng với ba vi khuẩn được xác định bằng MAG đại diện cho một dòng họ cấp độ họ mới trong bộ Brevinematales. Dựa trên các đặc điểm bộ gen và kiểu hình, xoắn khuẩn mới được đề xuất phân loại là Longinema margulisiae gen. nov., sp. nov. trong một họ mới, Longinemaceae fam. nov. Cả hai chủng được nuôi cấy, NST và N5R, đều là vi khuẩn dị dưỡng hữu cơ kỵ khí phát triển bằng cách lên men tinh bột và một số loại đường. Chúng có thể tạo thành các thể tròn bền vững trong điều kiện tăng trưởng không thuận lợi, có thể tồn tại tới 15 phút ở 95°C và có thể trở lại các tế bào xoắn ban đầu. Chúng tôi cho rằng các thể tròn có thể tạo điều kiện thuận lợi cho sự phân bố toàn cầu của dòng dõi này, được phát hiện từ các đặc điểm phân tử và sự xâm chiếm của các môi trường dưới bề mặt."} +{"idx": 1870007, "en": "Calcification in lung nodules usually indicates a benign lesion. Here, we report the case of a 59-year-old male patient with a well defined 30 mm calcified nodule in his right upper lung lobe and calcified mediastinal lymph nodes. The mass was diagnosed as adenocarcinoma by transbronchial biopsy. He received systemic chemotherapy, followed by lobectomy and mediastinal lymph node dissection. During surgery, the lymph nodes were tightly adherent to the superior vena cava with invasion of the vascular wall. Pathological diagnosis confirmed acinar adenocarcinoma and psammoma bodies (PBs). Immunohistochemical analysis revealed tumor cells positive for parathyroid hormone-related proteins 1 and 2. Calcification of primary lung adenocarcinoma is rare. We report a calcified lesion where the secretion of parathyroid hormone by the tumor may have caused the accumulation of PBs. Calcification of metastatic lymph nodes may increase the risk of adhesion, requiring care during surgery. KEY POINTS: Significant findings of the study Lung adenocarcinoma with extensive calcification in primary and metastatic lymph node lesions is rare and the mechanism involved is poorly understood. Of significance, calcification in our case was related to parathyroid hormone-related proteins 1 and 2 secreted by the tumor. What this study adds This study suggests the potential role of parathyroid hormone-related proteins in lung tumor calcification. The implications for clinicians are that calcified metastatic lymph nodes and tumors might be tightly fused to tissues. Therefore, surgery should be conducted with care.", "gemini": "Sự vôi hóa trong các nốt phổi thường chỉ ra một tổn thương lành tính. Ở đây, chúng tôi báo cáo trường hợp của một bệnh nhân nam 59 tuổi có một nốt vôi hóa rõ ràng 30 mm ở thùy trên phổi phải và các hạch bạch huyết trung thất bị vôi hóa. Khối u được chẩn đoán là ung thư tuyến biểu mô bằng sinh thiết qua phế quản. Ông đã được hóa trị toàn thân, sau đó cắt thùy phổi và cắt bỏ hạch bạch huyết trung thất. Trong quá trình phẫu thuật, các hạch bạch huyết dính chặt vào tĩnh mạch chủ trên với sự xâm lấn của thành mạch. Chẩn đoán bệnh lý xác nhận ung thư tuyến biểu mô dạng nang và các thể psammoma (PB). Phân tích miễn dịch mô miễn dịch cho thấy các tế bào khối u dương tính với protein liên quan đến hormone tuyến cận giáp 1 và 2. Sự vôi hóa của ung thư tuyến biểu mô phổi nguyên phát rất hiếm gặp. Chúng tôi báo cáo một tổn thương vôi hóa mà ở đó sự tiết hormone tuyến cận giáp của khối u có thể gây ra sự tích tụ của PB. Sự vôi hóa của các hạch bạch huyết di căn có thể làm tăng nguy cơ dính, cần phải cẩn thận trong khi phẫu thuật. ĐIỂM CHÍNH: Các phát hiện quan trọng của nghiên cứu Ung thư tuyến biểu mô phổi với tình trạng vôi hóa rộng rãi ở các tổn thương hạch bạch huyết nguyên phát và di căn rất hiếm gặp và cơ chế liên quan vẫn chưa được hiểu rõ. Điều đáng kể là tình trạng vôi hóa trong trường hợp của chúng tôi có liên quan đến protein liên quan đến hormone tuyến cận giáp 1 và 2 do khối u tiết ra. Nghiên cứu này bổ sung thêm điều gì Nghiên cứu này gợi ý vai trò tiềm ẩn của protein liên quan đến hormone tuyến cận giáp trong quá trình vôi hóa khối u phổi. Ý nghĩa đối với các bác sĩ lâm sàng là các hạch bạch huyết và khối u di căn bị vôi hóa có thể liên kết chặt chẽ với các mô. Do đó, cần tiến hành phẫu thuật một cách cẩn thận."} +{"idx": 1870008, "en": "PURPOSE To conduct a detailed analysis on the burden of vision loss due to diabetic retinopathy (DR) by year, age and gender in China from 1990 to 2017. METHODS This is a between-country, retrospective, comparative burden-of-disease study. Prevalence and Years Lived with Disability (YLDs) data caused by DR in China and relative territories were extracted from the Global Burden of Disease (GBD) study 2017 to observe the changing trends of vision loss. RESULTS Prevalence and YLDs of DR in China increased significantly from 1990 to 2017. The age-standardized prevalence and YLDs rate witnessed a slowly declining trend recently. Higher prevalence and YLDs were observed in female subjects in the past three decades. Middle-aged and elderly people suffered from a higher burden of DR-induced vision loss. The total age-standardized prevalence rate of China in 1990 (17.68) and 2017 (21.88) was lower than that of other seven neighbouring countries. In 2017, moderate vision loss (MVL) (17.19) accounted for the largest proportion in China. The all-age YLDs of DR showed the second-highest increase (150.26%) while the age-standardized YLDs rate showed the third-highest increase (14.91%) among fifteen common causes of vision impairment (VI) from 1990 to 2017. China ranked 3rd among G20 countries in terms of all-age YLDs while ranked 18th in age-standardized YLDs rate in 2017. CONCLUSIONS In the past three decades, the prevalence and YLDs due to DR in China have improved in both genders. This study highlights the importance of prevention for DR, especially for women and the elderly.", "gemini": "MỤC ĐÍCH Tiến hành phân tích chi tiết về gánh nặng mất thị lực do bệnh võng mạc do tiểu đường (DR) theo năm, tuổi và giới tính ở Trung Quốc từ năm 1990 đến 2017. PHƯƠNG PHÁP Đây là một nghiên cứu so sánh gánh nặng bệnh tật hồi cứu giữa các quốc gia. Dữ liệu về tỷ lệ mắc và số năm sống với tàn tật (YLD) do DR gây ra ở Trung Quốc và các vùng lãnh thổ liên quan được trích xuất từ nghiên cứu Gánh nặng bệnh tật toàn cầu (GBD) năm 2017 để quan sát xu hướng thay đổi của tình trạng mất thị lực. KẾT QUẢ Tỷ lệ mắc và YLD của DR ở Trung Quốc đã tăng đáng kể từ năm 1990 đến 2017. Tỷ lệ mắc và YLD chuẩn hóa theo tuổi gần đây đã chứng kiến xu hướng giảm chậm. Tỷ lệ mắc và YLD cao hơn được quan sát thấy ở các đối tượng nữ trong ba thập kỷ qua. Người trung niên và người cao tuổi phải chịu gánh nặng mất thị lực do DR cao hơn. Tổng tỷ lệ mắc chuẩn hóa theo tuổi của Trung Quốc năm 1990 (17,68) và năm 2017 (21,88) thấp hơn so với bảy quốc gia láng giềng khác. Năm 2017, mất thị lực vừa (MVL) (17,19) chiếm tỷ lệ lớn nhất ở Trung Quốc. YLD của DR ở mọi lứa tuổi cho thấy mức tăng cao thứ hai (150,26%) trong khi tỷ lệ YLD chuẩn hóa theo tuổi cho thấy mức tăng cao thứ ba (14,91%) trong số mười lăm nguyên nhân phổ biến gây suy giảm thị lực (VI) từ năm 1990 đến 2017. Trung Quốc đứng thứ 3 trong số các quốc gia G20 về YLD ở mọi lứa tuổi trong khi đứng thứ 18 về tỷ lệ YLD chuẩn hóa theo tuổi vào năm 2017. KẾT LUẬN Trong ba thập kỷ qua, tỷ lệ mắc và YLD do DR ở Trung Quốc đã được cải thiện ở cả hai giới. Nghiên cứu này nhấn mạnh tầm quan trọng của việc phòng ngừa DR, đặc biệt là đối với phụ nữ và người cao tuổi."} +{"idx": 1870009, "en": "INTRODUCTION Most clinical trials for new antiretroviral (ARV) agents are conducted among narrowly defined adult populations. Only after safety and efficacy have been clearly demonstrated among adults living with HIV are trials including adolescents, children and infants conducted. This approach contributes to significant delays in the availability of optimal new ARV regimens for infants, children and adolescents. This commentary discusses issues related to the inclusion of adolescents aged 12 to 18 years in initial HIV clinical phase 3 trials of novel antiretrovirals (ARVs) or conducting parallel phase 3 clinical trials among adolescents. DISCUSSION The absorption, metabolic and excretion or elimination pathways for drugs do not significantly differ between adolescents and adults. In fact, dosing recommendations for ARVs are the same for adults and adolescents who meet the age and weight criteria. Although conducting clinical trials among adolescents present special challenges (e.g. consenting minors and concerns about trial completion and contraception), these challenges can be addressed to obtain high-quality trial results. Importantly, new agents and optimized combinations have more favourable dosing schedules and side-effect profiles and are more effective ARV agents with higher HIV drug resistance thresholds, which would be extremely beneficial to improve outcomes among HIV-positive adolescents. CONCLUSIONS Adolescents may not present with significantly different pharmacokinetic characteristics from those in adults. Including HIV-positive adolescents in phase 3 ARV clinical trials, either with adults or in specific adolescent studies conducted in parallel, would allow adolescents to access promising, more effective treatment for HIV years earlier than with the current stepwise approach.", "gemini": "GIỚI THIỆU Hầu hết các thử nghiệm lâm sàng đối với các tác nhân thuốc kháng retrovirus (ARV) mới được tiến hành trong nhóm dân số người lớn được xác định một cách hẹp. Chỉ sau khi tính an toàn và hiệu quả đã được chứng minh rõ ràng ở những người lớn sống chung với HIV thì các thử nghiệm bao gồm cả thanh thiếu niên, trẻ em và trẻ sơ sinh mới được tiến hành. Cách tiếp cận này góp phần làm chậm đáng kể việc cung cấp các phác đồ ARV mới tối ưu cho trẻ sơ sinh, trẻ em và thanh thiếu niên. Bài bình luận này thảo luận về các vấn đề liên quan đến việc đưa những thanh thiếu niên từ 12 đến 18 tuổi vào các thử nghiệm lâm sàng giai đoạn 3 ban đầu về HIV của các loại thuốc kháng retrovirus mới (ARV) hoặc tiến hành các thử nghiệm lâm sàng giai đoạn 3 song song trong nhóm thanh thiếu niên. THẢO LUẬN Các con đường hấp thụ, chuyển hóa và bài tiết hoặc đào thải thuốc không khác biệt đáng kể giữa thanh thiếu niên và người lớn. Trên thực tế, các khuyến cáo về liều dùng ARV là giống nhau đối với người lớn và thanh thiếu niên đáp ứng các tiêu chí về tuổi và cân nặng. Mặc dù việc tiến hành các thử nghiệm lâm sàng trong nhóm thanh thiếu niên đặt ra những thách thức đặc biệt (ví dụ: trẻ vị thành niên đồng ý và lo ngại về việc hoàn thành thử nghiệm và tránh thai), nhưng có thể giải quyết những thách thức này để có được kết quả thử nghiệm chất lượng cao. Điều quan trọng là các tác nhân mới và các phối hợp tối ưu có các chương trình dùng thuốc và hồ sơ tác dụng phụ thuận lợi hơn và là các tác nhân ARV hiệu quả hơn với ngưỡng kháng thuốc HIV cao hơn, điều này sẽ cực kỳ có lợi để cải thiện kết quả ở những thanh thiếu niên dương tính với HIV. KẾT LUẬN Thanh thiếu niên có thể không biểu hiện các đặc điểm dược động học khác biệt đáng kể so với người lớn. Việc đưa những thanh thiếu niên dương tính với HIV vào các thử nghiệm lâm sàng ARV giai đoạn 3, tiến hành cùng với người lớn hoặc trong các nghiên cứu dành riêng cho thanh thiếu niên được tiến hành song song, sẽ cho phép thanh thiếu niên tiếp cận các phương pháp điều trị HIV hứa hẹn, hiệu quả hơn sớm hơn nhiều năm so với cách tiếp cận từng bước hiện tại."} +{"idx": 1870010, "en": "COFs were synthesized by a microwave-assisted solvothermal route, with the building blocks containing 1,3,5-tris(4-aminophenyl) benzene and 2,3,5,6-tetra-fluoroterephthalaldehyde (or 1,4-phthalaldehyde). The -F groups introduced into the benzene ring promoted hydrophobicity and stability of the COFs. The universality and long effectiveness of oil adsorption can be realized when applying COFs as adsorbent. The powder also exhibited excellent water-in-oil emulsions separation performance, with the separation efficiency no lower than 99.5%. In this work, the use of microwave solvothermal synthesis of superhydrophobic COFs is potential to replace the conventional synthesis process and more suitable for industrial scale-up production. Furthermore, the findings provide a new strategy for solving the problem of oil spill treatment and industrial water-in-oil emulsions separation by using the emerging 2D COFs.", "gemini": "COF được tổng hợp bằng phương pháp dung môi nhiệt hỗ trợ vi sóng, với các khối xây dựng chứa 1,3,5-tris(4-aminophenyl) benzen và 2,3,5,6-tetra-fluoroterephthalaldehyde (hoặc 1,4-phthalaldehyde). Các nhóm -F được đưa vào vòng benzen thúc đẩy tính kỵ nước và độ ổn định của COF. Tính phổ quát và hiệu quả lâu dài của quá trình hấp phụ dầu có thể được nhận ra khi sử dụng COF làm chất hấp phụ. Bột cũng thể hiện hiệu suất tách nhũ tương nước trong dầu tuyệt vời, với hiệu suất tách không thấp hơn 99,5%. Trong công trình này, việc sử dụng tổng hợp dung môi nhiệt vi sóng của COF siêu kỵ nước có khả năng thay thế quy trình tổng hợp thông thường và phù hợp hơn cho sản xuất ở quy mô công nghiệp. Hơn nữa, những phát hiện này cung cấp một chiến lược mới để giải quyết vấn đề xử lý tràn dầu và tách nhũ tương nước trong dầu trong công nghiệp bằng cách sử dụng COF 2D mới nổi."} +{"idx": 1870011, "en": "During the productive Paleoproterozoic (2.4-1.8 Ga) and less productive Mesoproterozoic (1.8-1.0 Ga), the ocean was suboxic to anoxic and multicellular organisms had not yet evolved. Here, we link geologic information about the Proterozoic ocean to microbial processes in modern low-oxygen systems. High iron concentrations and rates of Fe cycling in the Proterozoic are the largest differences from modern oxygen-deficient zones. In anoxic waters, which composed most of the Paleoproterozoic and ~40% of the Mesoproterozoic ocean, nitrogen cycling dominated. Rates of N2 production by denitrification and anammox were likely linked to sinking organic matter fluxes and in situ primary productivity under anoxic conditions. Additionally autotrophic denitrifiers could have used reduced iron or methane. 50% of the Mesoproterozoic ocean may have been suboxic, promoting nitrification and metal oxidation in the suboxic water and N2 O and N2 production by partial and complete denitrification in anoxic zones in organic aggregates. Sulfidic conditions may have composed ~10% of the Mesoproterozoic ocean focused along continental margins. Due to low nitrate concentrations in offshore regions, anammox bacteria likely dominated N2 production immediately above sulfidic zones, but in coastal regions, higher nitrate concentrations probably promoted complete S-oxidizing autotrophic denitrification at the sulfide interface.", "gemini": "Trong thời kỳ Cổ Nguyên sinh (2,4-1,8 Ga) và Trung Nguyên sinh (1,8-1,0 Ga) ít hiệu quả hơn, đại dương ở trạng thái thiếu oxy đến kỵ khí và các sinh vật đa bào vẫn chưa tiến hóa. Ở đây, chúng tôi liên kết thông tin địa chất về đại dương Nguyên sinh với các quá trình vi sinh vật trong các hệ thống ít oxy hiện đại. Nồng độ sắt cao và tỷ lệ tuần hoàn Fe trong thời kỳ Nguyên sinh là những điểm khác biệt lớn nhất so với các vùng thiếu oxy hiện đại. Trong vùng nước kỵ khí, chiếm phần lớn đại dương Cổ Nguyên sinh và ~40% đại dương Trung Nguyên sinh, chu trình nitơ chiếm ưu thế. Tỷ lệ sản xuất N2 do quá trình khử nitrat và anammox có khả năng liên quan đến dòng vật chất hữu cơ chìm và năng suất sơ cấp tại chỗ trong điều kiện kỵ khí. Ngoài ra, vi khuẩn khử nitrat tự dưỡng có thể sử dụng sắt hoặc metan bị khử. 50% đại dương Trung Nguyên sinh có thể là thiếu oxy, thúc đẩy quá trình nitrat hóa và oxy hóa kim loại trong nước thiếu oxy và sản xuất N2 O và N2 bằng quá trình khử nitrat một phần và hoàn toàn trong các vùng kỵ khí trong các tập hợp hữu cơ. Các điều kiện sunfua có thể chiếm khoảng 10% đại dương Trung Nguyên sinh tập trung dọc theo các rìa lục địa. Do nồng độ nitrat thấp ở các vùng xa bờ, vi khuẩn anammox có khả năng chiếm ưu thế trong quá trình sản xuất N2 ngay phía trên các vùng sunfua, nhưng ở các vùng ven biển, nồng độ nitrat cao hơn có thể thúc đẩy quá trình khử nitrat tự dưỡng oxy hóa S hoàn toàn ở giao diện sunfua."} +{"idx": 1870012, "en": "BACKGROUND Despite the recent development of molecular-targeted treatment and immunotherapy, survival of patients with esophageal adenocarcinoma (EAC) with poor prognosis is still poor due to lack of an effective biomarker. In this study, we aimed to explore the ceRNA and construct a multivariate gene expression predictor model using data from The Cancer Genome Atlas (TCGA) to predict the prognosis of EAC patients. METHODS We conducted differential expression analysis using mRNA, miRNA and lncRNA transciptome data from EAC and normal patients as well as corresponding clinical information from TCGA database, and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of those unique differentially expressed mRNAs using the Integrate Discovery Database (DAVID) database. We then constructed the lncRNA-miRNA-mRNA competing endogenous RNA (ceRNA) network of EAC and used Cox proportional hazard analysis to generate a multivariate gene expression predictor model. We finally performed survival analysis to determine the effect of differentially expressed mRNA on patients' overall survival and discover the hub gene. RESULTS We identified a total of 488 lncRNAs, 33 miRNAs, and 1207 mRNAs with differentially expressed profiles. Cox proportional hazard analysis and survival analysis using the ceRNA network revealed four genes (IL-11, PDGFD, NPTX1, ITPR1) as potential biomarkers of EAC prognosis in our predictor model, and IL-11 was identified as an independent prognostic factor. CONCLUSIONS In conclusion, we identified differences in the ceRNA regulatory networks and constructed a four-gene expression-based survival predictor model, which could be referential for future clinical research.", "gemini": "BỐI CẢNH Mặc dù phương pháp điều trị nhắm mục tiêu phân tử và liệu pháp miễn dịch đã có những tiến triển gần đây, nhưng khả năng sống sót của bệnh nhân ung thư biểu mô tuyến thực quản (EAC) có tiên lượng kém vẫn còn thấp do thiếu sinh học chỉ điểm hiệu quả. Trong nghiên cứu này, chúng tôi nhằm mục đích khám phá ceRNA và xây dựng mô hình dự báo biểu hiện gen đa biến bằng cách sử dụng dữ liệu từ The Cancer Genome Atlas (TCGA) để dự đoán tiên lượng của bệnh nhân EAC. PHƯƠNG PHÁP Chúng tôi đã tiến hành phân tích biểu hiện khác biệt bằng cách sử dụng dữ liệu phiên mã mRNA, miRNA và lncRNA từ bệnh nhân EAC và bệnh nhân bình thường cũng như thông tin lâm sàng tương ứng từ cơ sở dữ liệu TCGA và phân tích con đường của Bách khoa toàn thư gen và gen Kyoto (KEGG) của những mRNA biểu hiện khác biệt duy nhất đó bằng cơ sở dữ liệu Integrate Discovery Database (DAVID). Sau đó, chúng tôi xây dựng mạng RNA cạnh tranh nội sinh (ceRNA) lncRNA-miRNA-mRNA của EAC và sử dụng phân tích nguy cơ tỷ lệ Cox để tạo ra mô hình dự báo biểu hiện gen đa biến. Cuối cùng, chúng tôi đã thực hiện phân tích khả năng sống sót để xác định tác động của mRNA biểu hiện khác biệt đến khả năng sống sót chung của bệnh nhân và phát hiện ra gen trung tâm. KẾT QUẢ Chúng tôi đã xác định được tổng cộng 488 lncRNA, 33 miRNA và 1207 mRNA có cấu hình biểu hiện khác biệt. Phân tích nguy cơ tỷ lệ Cox và phân tích khả năng sống sót bằng cách sử dụng mạng ceRNA cho thấy bốn gen (IL-11, PDGFD, NPTX1, ITPR1) là các sinh học chỉ điểm tiềm năng về tiên lượng EAC trong mô hình dự báo của chúng tôi và IL-11 được xác định là một yếu tố tiên lượng độc lập. KẾT LUẬN Tóm lại, chúng tôi đã xác định được sự khác biệt trong các mạng điều hòa ceRNA và xây dựng một mô hình dự báo khả năng sống sót dựa trên biểu hiện của bốn gen, có thể tham khảo cho các nghiên cứu lâm sàng trong tương lai."} +{"idx": 1870013, "en": "BACKGROUND To evaluate the performance of transvaginal sonoelastography (TVSE) for differential diagnosis between malignant and benign cervical lesions using a meta-analysis. METHODS An independent literature search was conducted on the English medical database, including PubMed, Embase and Medline, Cochrane Library, Web of Science, and OVID. The diagnostic accuracy of TVSE was compared with that of histopathology, which is the gold reference standard for diagnosis. The accuracy of TVSE was assessed by calculating the pooled sensitivity, specificity, diagnostic odds ratio, and area under the curve (AUC). The imaging mechanisms, assessment methods, and QUADAS scores were assessed with a meta-regression analysis. A Deeks funnel plot was performed for evaluating publication bias. RESULTS Six eligible studies reported a total sample of 615 cervical lesions (415 cancers, 200 benign lesions). TVSE showed a pooled diagnostic odds ratio of 21.42 (95% CI 13.65-33.61), sensitivity of 0.87 (95% CI 0.84-0.90), specificity of 0.79 (95% CI 0.72-0.84), and an AUC of 0.892 (Q* = 0.822). The results of the meta-regression analysis showed that the imaging mechanism (P = .253), the assessment method (P = .279), or QUADAS score (P = .205) did not affect the study heterogeneity. CONCLUSION TVSE has a relatively high and satisfactory value for differential diagnosis between malignant and benign cervical lesions. The diagnostic performance of strain elastography and shear wave elastography were similar and good. However, to accommodate heterogeneity and publication bias, high-quality studies are required to further comparative effectiveness analyses to verify the efficacy of ultrasound detection.", "gemini": "BỐI CẢNH Đánh giá hiệu quả của siêu âm đàn hồi âm đạo (TVSE) trong chẩn đoán phân biệt giữa tổn thương cổ tử cung ác tính và lành tính bằng cách sử dụng phân tích tổng hợp. PHƯƠNG PHÁP Một tìm kiếm tài liệu độc lập đã được thực hiện trên cơ sở dữ liệu y học tiếng Anh, bao gồm PubMed, Embase và Medline, Thư viện Cochrane, Web of Science và OVID. Độ chính xác chẩn đoán của TVSE đã được so sánh với độ chính xác của mô bệnh học, đây là tiêu chuẩn vàng để chẩn đoán. Độ chính xác của TVSE được đánh giá bằng cách tính độ nhạy, độ đặc hiệu, tỷ lệ chẩn đoán và diện tích dưới đường cong (AUC) gộp. Các cơ chế hình ảnh, phương pháp đánh giá và điểm QUADAS được đánh giá bằng phân tích hồi quy tổng hợp. Đồ thị phễu Deeks đã được thực hiện để đánh giá độ lệch công bố. KẾT QUẢ Sáu nghiên cứu đủ điều kiện đã báo cáo tổng số 615 tổn thương cổ tử cung (415 ung thư, 200 tổn thương lành tính). TVSE cho thấy tỷ lệ chẩn đoán gộp là 21,42 (95% CI 13,65-33,61), độ nhạy là 0,87 (95% CI 0,84-0,90), độ đặc hiệu là 0,79 (95% CI 0,72-0,84) và AUC là 0,892 (Q* = 0,822). Kết quả của phân tích hồi quy tổng hợp cho thấy cơ chế hình ảnh (P = .253), phương pháp đánh giá (P = .279) hoặc điểm QUADAS (P = .205) không ảnh hưởng đến tính không đồng nhất của nghiên cứu. KẾT LUẬN TVSE có giá trị tương đối cao và khả quan trong chẩn đoán phân biệt giữa tổn thương cổ tử cung ác tính và lành tính. Hiệu quả chẩn đoán của siêu âm đàn hồi biến dạng và siêu âm đàn hồi sóng cắt là tương tự và tốt. Tuy nhiên, để giải quyết tính không đồng nhất và độ lệch công bố, cần có các nghiên cứu chất lượng cao để phân tích hiệu quả so sánh thêm để xác minh hiệu quả của phát hiện siêu âm."} +{"idx": 1870014, "en": "In this work, we developed an experimental-computational analysis framework which facilitated objective, quantitative, standardised, methodological, and systematic comparisons between the biomechanical efficacies of two fundamentally different dressing technologies for pressure ulcer prevention: A dressing technology based on cellulose fibres used as the core matrix was evaluated vs the conventional silicone-foam dressing design concept, which was represented by multiple products which belong in this category. Using an anatomically-realistic computer (finite element) model of a supine female patient to whom the different sacral dressings have been applied virtually, we quantitatively evaluated the efficacy of the different dressings by means of a set of 3 biomechanical indices: The protective efficacy index, the protective endurance, and the prophylactic trade-off design parameter. Prior rigorous experimental measurements of the physical and mechanical behaviours and properties of each tested dressing, including tensile, compressive, and friction properties, have been conducted and used as inputs for the computer modelling. Each dressing was evaluated for its tissue protection performances at a new (from the package) state, as well as after exposure to moisture conditions simulating wet bedsheets. Our results demonstrated that the dressing with the fluff core is at least as-good as silicone-foams but importantly, provides the best balance between protective performances at its \"new\" condition and the performance after being exposed to moisture. We conclude that preventative dressings are not equal in their prophylactic performances, but rather, the base technology, the ingredients, and their arrangement in the dressing structure shape the quality of the delivered tissue protection.", "gemini": "Trong công trình này, chúng tôi đã phát triển một khuôn khổ phân tích tính toán thực nghiệm giúp tạo điều kiện cho các so sánh khách quan, định lượng, tiêu chuẩn hóa, phương pháp luận và có hệ thống giữa các hiệu quả cơ sinh học của hai công nghệ băng vết thương cơ bản khác nhau để phòng ngừa loét tì đè: Một công nghệ băng vết thương dựa trên sợi cellulose được sử dụng làm ma trận cốt lõi đã được đánh giá so với khái niệm thiết kế băng vết thương silicone-bọt thông thường, được thể hiện bằng nhiều sản phẩm thuộc loại này. Sử dụng mô hình máy tính (phần tử hữu hạn) thực tế về mặt giải phẫu của một bệnh nhân nữ nằm ngửa đã được áp dụng các loại băng vết thương vùng cùng cụt khác nhau, chúng tôi đã đánh giá định lượng hiệu quả của các loại băng vết thương khác nhau bằng một tập hợp 3 chỉ số cơ sinh học: Chỉ số hiệu quả bảo vệ, độ bền bảo vệ và thông số thiết kế đánh đổi phòng ngừa. Các phép đo thực nghiệm nghiêm ngặt trước đó về các hành vi và đặc tính vật lý và cơ học của từng loại băng vết thương được thử nghiệm, bao gồm các đặc tính về độ bền kéo, độ nén và ma sát, đã được thực hiện và sử dụng làm đầu vào cho mô hình máy tính. Mỗi loại băng vết thương đã được đánh giá về hiệu suất bảo vệ mô ở trạng thái mới (từ bao bì) cũng như sau khi tiếp xúc với điều kiện ẩm mô phỏng ga trải giường ướt. Kết quả của chúng tôi chứng minh rằng băng vết thương có lõi xốp ít nhất cũng tốt như băng silicone-bọt nhưng quan trọng hơn là cung cấp sự cân bằng tốt nhất giữa hiệu suất bảo vệ ở trạng thái \"mới\" và hiệu suất sau khi tiếp xúc với độ ẩm. Chúng tôi kết luận rằng các loại băng vết thương phòng ngừa không giống nhau về hiệu suất phòng ngừa của chúng, mà là công nghệ cơ bản, các thành phần và sự sắp xếp của chúng trong cấu trúc băng vết thương quyết định chất lượng của lớp bảo vệ mô được cung cấp."} +{"idx": 1870015, "en": "BACKGROUND Familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC) is an autosomal recessive tubulopathy characterized by excessive urinary wasting of magnesium and calcium, bilateral nephrocalcinosis, and progressive chronic renal failure in childhood or adolescence. FHHNC is caused by mutations in CLDN16 and CLDN19, which encode the tight-junction proteins claudin-16 and claudin-19, respectively. Most of these mutations are missense mutations and large deletions are rare. METHODS We examined the clinical and biochemical features of a Spanish boy with early onset of FHHNC symptoms. Exons and flanking intronic segments of CLDN16 and CLDN19 were analyzed by direct sequencing. We developed a new assay based on Quantitative Multiplex PCR of Short Fluorescent Fragments (QMPSF) to investigate large CLDN16 deletions. RESULTS Genetic analysis revealed two novel compound heterozygous mutations of CLDN16, comprising a missense mutation, c.277G>A; p.(Ala93Thr), in one allele, and a gross deletion that lacked exons 4 and 5,c.(840+25_?)del, in the other allele. The patient inherited these variants from his mother and father, respectively. CONCLUSIONS Using direct sequencing and our QMPSF assay, we identified the genetic cause of FHHNC in our patient. This QMPSF assay should facilitate the genetic diagnosis of FHHNC. Our study provided additional data on the genotypic spectrum of the CLDN16 gene.", "gemini": "BỐI CẢNH Thiểu magiê máu gia đình với tăng calci niệu và vôi hóa thận (FHHNC) là một bệnh lý ống thận lặn trên nhiễm sắc thể thường đặc trưng bởi sự bài tiết quá mức magiê và canxi qua nước tiểu, vôi hóa thận hai bên và suy thận mạn tính tiến triển ở trẻ em hoặc thanh thiếu niên. FHHNC là do các đột biến ở CLDN16 và CLDN19, lần lượt mã hóa các protein liên kết chặt là claudin-16 và claudin-19. Hầu hết các đột biến này là đột biến sai nghĩa và các mất đoạn lớn rất hiếm gặp. PHƯƠNG PHÁP Chúng tôi đã kiểm tra các đặc điểm lâm sàng và sinh hóa của một bé trai người Tây Ban Nha khởi phát sớm các triệu chứng FHHNC. Các exon và đoạn intron bên cạnh của CLDN16 và CLDN19 đã được phân tích bằng giải trình tự trực tiếp. Chúng tôi đã phát triển một xét nghiệm mới dựa trên Phản ứng chuỗi trùng hợp đa kênh định lượng các đoạn huỳnh quang ngắn (QMPSF) để nghiên cứu các mất đoạn CLDN16 lớn. KẾT QUẢ Phân tích di truyền cho thấy hai đột biến dị hợp tử kép mới của CLDN16, bao gồm một đột biến sai nghĩa, c.277G>A; p.(Ala93Thr), ở một alen và một mất đoạn lớn thiếu các exon 4 và 5, c.(840+25_?)del, ở alen còn lại. Bệnh nhân thừa hưởng các biến thể này lần lượt từ mẹ và cha của mình. KẾT LUẬN Sử dụng giải trình tự trực tiếp và xét nghiệm QMPSF của chúng tôi, chúng tôi đã xác định được nguyên nhân di truyền của FHHNC ở bệnh nhân của chúng tôi. Xét nghiệm QMPSF này sẽ giúp chẩn đoán di truyền FHHNC dễ dàng hơn. Nghiên cứu của chúng tôi cung cấp thêm dữ liệu về phổ kiểu gen của gen CLDN16."} +{"idx": 1870016, "en": "One of the challenges in the design of confirmatory trials is to deal with uncertainties regarding the optimal target population for a novel drug. Adaptive enrichment designs (AED) which allow for a data-driven selection of one or more prespecified biomarker subpopulations at an interim analysis have been proposed in this setting but practical case studies of AEDs are still relatively rare. We present the design of an AED with a binary endpoint in the highly dynamic setting of cancer immunotherapy. The trial was initiated as a conventional trial in early triple-negative breast cancer but amended to an AED based on emerging data external to the trial suggesting that PD-L1 status could be a predictive biomarker. Operating characteristics are discussed including the concept of a minimal detectable difference, that is, the smallest observed treatment effect that would lead to a statistically significant result in at least one of the target populations at the interim or the final analysis, respectively, in the setting of AED.", "gemini": "Một trong những thách thức trong thiết kế các thử nghiệm xác nhận là giải quyết những bất trắc liên quan đến quần thể mục tiêu tối ưu cho một loại thuốc mới. Các thiết kế làm giàu thích ứng (AED) cho phép lựa chọn theo dữ liệu một hoặc nhiều phân nhóm sinh học đã xác định trước tại phân tích tạm thời đã được đề xuất trong bối cảnh này nhưng các nghiên cứu trường hợp thực tế về AED vẫn còn tương đối hiếm. Chúng tôi trình bày thiết kế của một AED với điểm cuối nhị phân trong bối cảnh rất năng động của liệu pháp miễn dịch ung thư. Thử nghiệm đã được khởi xướng như một thử nghiệm thông thường ở giai đoạn đầu ung thư vú ba âm tính nhưng đã được sửa đổi thành AED dựa trên dữ liệu mới nổi bên ngoài thử nghiệm cho thấy tình trạng PD-L1 có thể là một sinh học đánh dấu dự đoán. Các đặc điểm hoạt động được thảo luận bao gồm khái niệm về sự khác biệt tối thiểu có thể phát hiện được, tức là hiệu ứng điều trị nhỏ nhất được quan sát thấy có thể dẫn đến kết quả có ý nghĩa thống kê ở ít nhất một trong các quần thể mục tiêu tại phân tích tạm thời hoặc phân tích cuối cùng, tương ứng, trong bối cảnh AED."} +{"idx": 1870017, "en": "INTRODUCTION HIV continues to devastate the adolescent population in sub-Saharan Africa (SSA). The complex array of interpersonal, social, structural and system-level obstacles specific to adolescents have slowed progress in prevention and treatment of HIV in this population. The field of implementation science holds promise for addressing these challenges. DISCUSSION There is growing consensus that enhanced interactions between researchers and users of scientific evidence are important and necessary to tackle enduring barriers to implementation. In 2017, the Fogarty International Center launched the Adolescent HIV Prevention and Treatment Implementation Science Alliance (AHISA) to promote communication and catalyse collaboration among implementation scientists and implementers to enhance the cross-fertilization of insights as research advances and the implementation environment evolves. This network has identified key implementation science questions for adolescent HIV, assessed how members' research is addressing them, and is currently conducting a concept mapping exercise to more systematically identify implementation research priorities. In addition, AHSA pinpointed common challenges to addressing these questions and discussed their collective capacity to conduct implementation science using the shared learning approach of the network. Specifically, AHISA addresses challenges related to capacity building, developing mentorship, engaging stakeholders, and involving adolescents through support for training efforts and funding region-/country-specific networks that respond to local issues and increase implementation science capacity across SSA. CONCLUSIONS Innovative platforms, like AHISA, that foster collaborations between implementation science researchers, policymakers and community participants to prioritizes research needs and identify and address implementation challenges can speed the translation of effective HIV interventions to benefit adolescent health.", "gemini": "GIỚI THIỆU HIV tiếp tục tàn phá dân số thanh thiếu niên ở Châu Phi cận Sahara (SSA). Mảng phức hợp các trở ngại liên cá nhân, xã hội, cấu trúc và cấp hệ thống dành riêng cho thanh thiếu niên đã làm chậm tiến độ phòng ngừa và điều trị HIV trong nhóm dân số này. Lĩnh vực khoa học triển khai hứa hẹn sẽ giải quyết những thách thức này. THẢO LUẬN Có sự đồng thuận ngày càng tăng rằng sự tương tác nâng cao giữa các nhà nghiên cứu và người sử dụng bằng chứng khoa học là quan trọng và cần thiết để giải quyết các rào cản lâu dài đối với việc triển khai. Năm 2017, Trung tâm Quốc tế Fogarty đã ra mắt Liên minh Khoa học Triển khai Phòng ngừa và Điều trị HIV cho Thanh thiếu niên (AHISA) để thúc đẩy giao tiếp và xúc tác sự hợp tác giữa các nhà khoa học triển khai và những người triển khai nhằm tăng cường sự giao thoa của những hiểu biết khi nghiên cứu tiến triển và môi trường triển khai thay đổi. Mạng lưới này đã xác định các câu hỏi khoa học triển khai chính cho HIV ở thanh thiếu niên, đánh giá cách nghiên cứu của các thành viên giải quyết các câu hỏi đó và hiện đang tiến hành một bài tập lập bản đồ khái niệm để xác định ưu tiên nghiên cứu triển khai một cách có hệ thống hơn. Ngoài ra, AHISA đã xác định những thách thức chung trong việc giải quyết các câu hỏi này và thảo luận về khả năng chung của họ trong việc tiến hành khoa học triển khai bằng cách sử dụng phương pháp học tập chia sẻ của mạng lưới. Cụ thể, AHISA giải quyết các thách thức liên quan đến việc xây dựng năng lực, phát triển sự cố vấn, thu hút các bên liên quan và liên quan đến thanh thiếu niên thông qua hỗ trợ các nỗ lực đào tạo và tài trợ cho các mạng lưới cụ thể của khu vực/quốc gia để giải quyết các vấn đề tại địa phương và tăng cường năng lực khoa học triển khai trên khắp SSA. KẾT LUẬN Các nền tảng sáng tạo, như AHISA, thúc đẩy sự hợp tác giữa các nhà nghiên cứu khoa học triển khai, các nhà hoạch định chính sách và những người tham gia cộng đồng để ưu tiên nhu cầu nghiên cứu và xác định cũng như giải quyết các thách thức triển khai có thể đẩy nhanh quá trình chuyển đổi các can thiệp HIV hiệu quả để mang lại lợi ích cho sức khỏe của thanh thiếu niên."} +{"idx": 1870018, "en": "BACKGROUND Paediatric obesity poses dangers to children's short and long-term health. Multi-level ecological models posit how children's health behaviours are influenced by interpersonal relationships. OBJECTIVES To identify profiles of individual and interpersonal health behaviours and parenting skills among caregivers and their children with elevated BMI. METHODS Participants were 240 children (63.7% Latino) ages 5 to 12 years with body mass index ≥85th percentile and their caregivers in a paediatric weight management intervention trial. A latent profile analysis was used to identify profiles among caregiver report of parenting skills; child physical activity, eating behaviours, and food and beverage choices; family mealtime, media and sleep routines; and parent health behaviours, and associations with food and housing insecurity. RESULTS A three-class model was chosen based on conceptual interpretation and model fit. Profiles were differentiated by parenting skills, child food choices, child physical activity habits, family mealtime, media, and sleep routines, and parent health behaviours. Food and housing insecurity were associated with class membership while child and caregiver anthropometrics were not. CONCLUSIONS Distinct profiles existed among this low-income, racially/ethnically diverse sample of children with elevated BMI. Such findings emphasize the importance of assessing individual and interpersonal influences and contextual factors on childhood obesity.", "gemini": "BỐI CẢNH Béo phì ở trẻ em gây ra nguy hiểm cho sức khỏe của trẻ trong thời gian ngắn và dài hạn. Các mô hình sinh thái đa cấp cho thấy hành vi sức khỏe của trẻ em bị ảnh hưởng như thế nào bởi các mối quan hệ giữa các cá nhân. MỤC TIÊU Xác định hồ sơ về hành vi sức khỏe của cá nhân và giữa các cá nhân và các kỹ năng làm cha mẹ giữa những người chăm sóc và con cái của họ có chỉ số BMI cao. PHƯƠNG PHÁP Những người tham gia là 240 trẻ em (63,7% là người gốc Latinh) từ 5 đến 12 tuổi có chỉ số khối cơ thể ≥85 phần trăm và những người chăm sóc của chúng trong thử nghiệm can thiệp quản lý cân nặng nhi khoa. Phân tích hồ sơ tiềm ẩn được sử dụng để xác định hồ sơ trong số các báo cáo của người chăm sóc về các kỹ năng làm cha mẹ; hoạt động thể chất của trẻ, hành vi ăn uống và lựa chọn thực phẩm và đồ uống; bữa ăn gia đình, phương tiện truyền thông và thói quen ngủ; và hành vi sức khỏe của cha mẹ và mối liên quan với tình trạng mất an ninh lương thực và nhà ở. KẾT QUẢ Một mô hình ba lớp được chọn dựa trên diễn giải khái niệm và sự phù hợp của mô hình. Hồ sơ được phân biệt theo các kỹ năng làm cha mẹ, lựa chọn thực phẩm của trẻ, thói quen hoạt động thể chất của trẻ, bữa ăn gia đình, phương tiện truyền thông và thói quen ngủ, cũng như hành vi sức khỏe của cha mẹ. Tình trạng mất an ninh lương thực và nhà ở có liên quan đến tư cách thành viên của lớp học trong khi các phép đo nhân trắc học của trẻ và người chăm sóc thì không. KẾT LUẬN Các hồ sơ riêng biệt đã tồn tại trong nhóm trẻ có thu nhập thấp, đa dạng về chủng tộc/dân tộc này có chỉ số BMI cao. Những phát hiện như vậy nhấn mạnh tầm quan trọng của việc đánh giá các ảnh hưởng của cá nhân và giữa các cá nhân và các yếu tố bối cảnh đối với tình trạng béo phì ở trẻ em."} +{"idx": 1870019, "en": "INTRODUCTION Sustaining optimal adherence is the major challenge facing adolescents living with HIV (ALHIV), particularly in low-resource settings, where \"second-line\" is often the last accessible treatment option. We explored the knowledge and skills adolescents need in order to maintain improved adherence behaviours, and the specific ways clinicians and caregivers may support young people to do so more independently. METHODS We conducted individual, in-depth interviews with 20 ALHIV aged 10 to 18 years in Uganda in 2017 to 2018. All participants had recently commenced second-line treatment as part of a clinical trial. We used thematic qualitative analysis to examine adherence experiences and challenges while on first-line therapy, as well as specific supports necessary to optimise treatment-taking longer-term. RESULTS Adherence difficulties are exacerbated by relatively rapid shifts from caregiver-led approaches during childhood, to an expectation of autonomous treatment-taking with onset of adolescence. For many participants this shift compounded their ongoing struggles managing physical side effects and poor treatment literacy. Switching to second-line typically prompted reversion back to supervised adherence, with positive impacts on self-reported adherence in the immediate term. However, this measure is unlikely to be sustainable for caregivers due to significant caregiver burden (as on first line), and provided little opportunity for clinicians to guide and develop young people's capacity to successfully adopt responsibility for their own treatment-taking. CONCLUSIONS As ALHIV in sub-Saharan Africa are attributed increasing responsibility for treatment adherence and HIV management, they must be equipped with the core knowledge and skills required for successful, self-directed care. Young people need to be relationally supported to develop necessary \"adherence competencies\" within the supportive framework of a gradual \"transition\" period. Clinic conversations during this period should be adolescent-focussed and collaborative, and treatment-taking strategies situated within the context of their lived environments and support networks, to facilitate sustained adherence. The disclosure of adherence difficulties must be encouraged so that issues can be identified and addressed prior to treatment failure.", "gemini": "GIỚI THIỆU Duy trì tuân thủ tối ưu là thách thức chính mà những thanh thiếu niên sống chung với HIV (ALHIV) phải đối mặt, đặc biệt là ở những nơi có nguồn lực thấp, nơi mà \"phương pháp điều trị dự phòng\" thường là lựa chọn điều trị cuối cùng có thể tiếp cận được. Chúng tôi đã khám phá những kiến thức và kỹ năng mà thanh thiếu niên cần để duy trì hành vi tuân thủ được cải thiện và những cách cụ thể mà các bác sĩ lâm sàng và người chăm sóc có thể hỗ trợ các bạn trẻ thực hiện điều đó một cách độc lập hơn. PHƯƠNG PHÁP Chúng tôi đã tiến hành các cuộc phỏng vấn chuyên sâu, riêng lẻ với 20 ALHIV từ 10 đến 18 tuổi tại Uganda vào năm 2017 đến 2018. Tất cả những người tham gia gần đây đã bắt đầu điều trị dự phòng như một phần của thử nghiệm lâm sàng. Chúng tôi đã sử dụng phân tích định tính theo chủ đề để kiểm tra các kinh nghiệm và thách thức về sự tuân thủ trong khi điều trị bằng liệu pháp điều trị đầu tay, cũng như các hỗ trợ cụ thể cần thiết để tối ưu hóa việc điều trị lâu dài. KẾT QUẢ Những khó khăn trong việc tuân thủ trở nên trầm trọng hơn do sự thay đổi tương đối nhanh chóng từ các cách tiếp cận do người chăm sóc dẫn đầu trong thời thơ ấu sang kỳ vọng tự chủ trong việc điều trị khi bước vào tuổi vị thành niên. Đối với nhiều người tham gia, sự thay đổi này làm trầm trọng thêm những khó khăn liên tục của họ trong việc kiểm soát các tác dụng phụ về thể chất và kiến thức kém về điều trị. Việc chuyển sang phương pháp điều trị dự phòng thường thúc đẩy sự trở lại tuân thủ có sự giám sát, với những tác động tích cực đến việc tuân thủ được tự báo cáo trong thời gian trước mắt. Tuy nhiên, biện pháp này khó có thể bền vững đối với những người chăm sóc do gánh nặng chăm sóc đáng kể (như trong điều trị đầu tay) và ít có cơ hội cho các bác sĩ lâm sàng hướng dẫn và phát triển năng lực của những người trẻ tuổi để tự chịu trách nhiệm về việc điều trị của chính mình. KẾT LUẬN Khi ALHIV ở Châu Phi cận Sahara được giao trách nhiệm ngày càng tăng đối với việc tuân thủ điều trị và quản lý HIV, họ phải được trang bị những kiến thức và kỹ năng cốt lõi cần thiết để tự chăm sóc thành công. Những người trẻ tuổi cần được hỗ trợ về mặt mối quan hệ để phát triển \"năng lực tuân thủ\" cần thiết trong khuôn khổ hỗ trợ của giai đoạn \"chuyển tiếp\" dần dần. Các cuộc trò chuyện tại phòng khám trong giai đoạn này nên tập trung vào thanh thiếu niên và mang tính hợp tác, đồng thời các chiến lược điều trị phải nằm trong bối cảnh môi trường sống và mạng lưới hỗ trợ của họ để tạo điều kiện thuận lợi cho việc tuân thủ lâu dài. Việc tiết lộ những khó khăn trong việc tuân thủ phải được khuyến khích để các vấn đề có thể được xác định và giải quyết trước khi điều trị thất bại."} +{"idx": 1870020, "en": "BACKGROUND AND OBJECTIVES The aim of the present study was to establish and evaluate a new interprofessional teaching concept on the topic of wound management. METHODS After determining the status quo using a survey among medical students, we developed a new teaching concept that included a 150-minute course aimed at providing students with the opportunity to gain hands-on wound management skills. This interprofessional course was offered at the existing 'SkillsLab' teaching facility. The participants' subjective level of knowledge was assessed by questionnaire before and after the course. RESULTS Our survey among 190 medical students showed them to be very interested in gaining practical experience in the field of wound management. To date, 120 participants (54.8 % medical students; 45.2 % nursing students) have attended this new interprofessional course, which has been equally well received by both medical and nursing students. For all specific topics (diagnosis, treatment, use of wound dressings, debridement), course participation was associated with a significant increase in knowledge. CONCLUSION Given its relevance in clinical practice, it is important for medical students to learn about the various aspects associated with the care of patients with chronic wounds. By offering new teaching concepts, dermatology in particular is well suited to help students gain a better understanding of the challenges related to wound management and to improve their practical skills. Wound management is an ideal topic for interprofessional learning.", "gemini": "BỐI CẢNH VÀ MỤC TIÊU Mục đích của nghiên cứu hiện tại là thiết lập và đánh giá một khái niệm giảng dạy liên ngành mới về chủ đề quản lý vết thương. PHƯƠNG PHÁP Sau khi xác định tình trạng hiện tại bằng cách khảo sát các sinh viên y khoa, chúng tôi đã phát triển một khái niệm giảng dạy mới bao gồm một khóa học kéo dài 150 phút nhằm mục đích cung cấp cho sinh viên cơ hội để có được các kỹ năng quản lý vết thương thực hành. Khóa học liên ngành này được cung cấp tại cơ sở giảng dạy 'SkillsLab' hiện có. Trình độ kiến thức chủ quan của những người tham gia được đánh giá bằng bảng câu hỏi trước và sau khóa học. KẾT QUẢ Cuộc khảo sát của chúng tôi trên 190 sinh viên y khoa cho thấy họ rất quan tâm đến việc có được kinh nghiệm thực tế trong lĩnh vực quản lý vết thương. Cho đến nay, 120 người tham gia (54,8% là sinh viên y khoa; 45,2% là sinh viên điều dưỡng) đã tham dự khóa học liên ngành mới này, khóa học này được cả sinh viên y khoa và điều dưỡng đón nhận như nhau. Đối với tất cả các chủ đề cụ thể (chẩn đoán, điều trị, sử dụng băng vết thương, cắt lọc), việc tham gia khóa học có liên quan đến sự gia tăng đáng kể về kiến thức. KẾT LUẬN Do có liên quan trong thực hành lâm sàng, nên điều quan trọng là sinh viên y khoa phải tìm hiểu về các khía cạnh khác nhau liên quan đến việc chăm sóc bệnh nhân bị vết thương mãn tính. Bằng cách cung cấp các khái niệm giảng dạy mới, da liễu nói riêng rất phù hợp để giúp sinh viên hiểu rõ hơn về những thách thức liên quan đến quản lý vết thương và nâng cao các kỹ năng thực hành của họ. Quản lý vết thương là một chủ đề lý tưởng để học tập liên ngành."} +{"idx": 1870021, "en": "This review examined the risk of cardiovascular disease in adults with metabolically healthy overweight/obesity. A systematic review and meta-analysis using data from Medline, EMBASE, SCOPUS and Cochrane Library searched from inception up to 31st October 2019. We included prospective cohort studies of adults who are metabolically healthy or unhealthy. Outcomes were fatal and nonfatal cardiovascular events, all-cause mortality. Pooled relative risk was calculated for each outcome in populations with metabolically healthy overweight and metabolically healthy obesity using metabolically healthy normal weight as reference. A random-effects model was used for meta-analysis, and risk of bias assessment tool for nonrandomized studies assessed risk of bias within each study. Twenty-three prospective cohort studies with 4,492,723 participants were included. Cardiovascular disease risk was increased in metabolically healthy groups with overweight (RR = 1.34, CI: 1.23-1.46, n = 20, I2 = 90.3%) and obesity (RR = 1.58, CI: 1.34-1.85, n = 21, I2 = 92.2) compared with a reference group with metabolically healthy normal weight. Cardiovascular disease risk was similar irrespective of the number of risk factors used to define metabolically healthy and the risk remained in the group with no metabolic risk factors. Cardiovascular disease risk is increased in populations with overweight and obesity classified as metabolically healthy even when there were no metabolic risk factors.", "gemini": "Đánh giá này đã xem xét nguy cơ mắc bệnh tim mạch ở những người trưởng thành thừa cân/béo phì nhưng vẫn khỏe mạnh về mặt chuyển hóa. Một đánh giá có hệ thống và phân tích tổng hợp sử dụng dữ liệu từ Medline, EMBASE, SCOPUS và Thư viện Cochrane được tìm kiếm từ khi bắt đầu đến ngày 31 tháng 10 năm 2019. Chúng tôi đã đưa vào các nghiên cứu theo dõi triển vọng về những người trưởng thành khỏe mạnh hoặc không khỏe mạnh về mặt chuyển hóa. Các kết quả là các biến cố tim mạch gây tử vong và không gây tử vong, tử vong do mọi nguyên nhân. Nguy cơ tương đối gộp được tính toán cho từng kết cục trong quần thể thừa cân khỏe mạnh về mặt chuyển hóa và béo phì khỏe mạnh về mặt chuyển hóa sử dụng cân nặng bình thường khỏe mạnh về mặt chuyển hóa làm tham chiếu. Mô hình hiệu ứng ngẫu nhiên được sử dụng cho phân tích tổng hợp và công cụ đánh giá nguy cơ thiên vị đối với các nghiên cứu không ngẫu nhiên đã đánh giá nguy cơ thiên vị trong mỗi nghiên cứu. Hai mươi ba nghiên cứu theo dõi triển vọng với 4.492.723 người tham gia đã được đưa vào. Nguy cơ mắc bệnh tim mạch tăng lên ở các nhóm khỏe mạnh về mặt chuyển hóa có tình trạng thừa cân (RR = 1,34, CI: 1,23-1,46, n = 20, I2 = 90,3%) và béo phì (RR = 1,58, CI: 1,34-1,85, n = 21, I2 = 92,2) so với nhóm tham chiếu có cân nặng bình thường khỏe mạnh về mặt chuyển hóa. Nguy cơ mắc bệnh tim mạch là tương tự bất kể số lượng yếu tố nguy cơ được sử dụng để xác định tình trạng khỏe mạnh về mặt chuyển hóa và nguy cơ vẫn tồn tại trong nhóm không có yếu tố nguy cơ chuyển hóa. Nguy cơ mắc bệnh tim mạch tăng lên ở những quần thể thừa cân và béo phì được phân loại là khỏe mạnh về mặt chuyển hóa ngay cả khi không có yếu tố nguy cơ chuyển hóa nào."} +{"idx": 1870022, "en": "BACKGROUND Neurofibromatosis type 1 (NF1) is a tumor-predisposition disorder that arises due to pathogenic variants in tumor suppressor NF1. NF1 has variable expressivity that may be due, at least in part, from heritable elements such as modifier genes; however, few genetic modifiers have been identified to date. METHODS In this study, we performed a genome-wide association analysis of the number of café-au-lait macules (CALM) that are considered a tumor-like trait as a clinical phenotype modifying NF1. RESULTS A borderline genome-wide significant association was identified in the discovery cohort (CALM1, N = 112) between CALM number and rs12190451 (and rs3799603, r2  = 1.0; p = 7.4 × 10-8 ) in the intronic region of RPS6KA2. Although, this association was not replicated in the second cohort (CALM2, N = 59) and a meta-analysis did not show significantly associated variants in this region, a significant corroboration score (0.72) was obtained for the RPS6KA2 signal in the discovery cohort (CALM1) using Complementary Pairs Stability Selection for Genome-Wide Association Studies (ComPaSS-GWAS) analysis, suggesting that the lack of replication may be due to heterogeneity of the cohorts rather than type I error. CONCLUSION rs12190451 is located in a melanocyte-specific enhancer and may influence RPS6KA2 expression in melanocytes-warranting further functional studies.", "gemini": "BỐI CẢNH Bệnh u sợi thần kinh loại 1 (NF1) là một rối loạn dễ mắc khối u phát sinh do các biến thể gây bệnh ở gen ức chế khối u NF1. NF1 có biểu hiện thay đổi có thể là do, ít nhất một phần, từ các yếu tố di truyền như gen điều biến; tuy nhiên, cho đến nay, người ta mới chỉ xác định được một số ít gen điều biến. PHƯƠNG PHÁP Trong nghiên cứu này, chúng tôi đã tiến hành phân tích liên kết toàn bộ hệ gen về số lượng các nốt cà phê sữa (CALM) được coi là một đặc điểm giống khối u như một kiểu hình lâm sàng làm thay đổi NF1. KẾT QUẢ Một liên kết toàn bộ hệ gen có ý nghĩa ranh giới đã được xác định trong nhóm nghiên cứu khám phá (CALM1, N = 112) giữa số lượng CALM và rs12190451 (và rs3799603, r2  = 1,0; p = 7,4 × 10-8 ) trong vùng intron của RPS6KA2. Mặc dù liên kết này không được lặp lại trong nhóm nghiên cứu thứ hai (CALM2, N = 59) và phân tích tổng hợp không cho thấy các biến thể có liên quan đáng kể trong vùng này, nhưng một điểm xác nhận có ý nghĩa (0,72) đã thu được cho tín hiệu RPS6KA2 trong nhóm nghiên cứu khám phá (CALM1) bằng cách sử dụng Phân tích lựa chọn độ ổn định của cặp bổ sung cho các nghiên cứu liên kết toàn bộ hệ gen (ComPaSS-GWAS), cho thấy rằng việc không lặp lại có thể là do tính không đồng nhất của các nhóm nghiên cứu chứ không phải là lỗi loại I. KẾT LUẬN rs12190451 nằm trong một vùng tăng cường đặc hiệu của tế bào hắc tố và có thể ảnh hưởng đến biểu hiện RPS6KA2 trong tế bào hắc tố-cần nghiên cứu chức năng sâu hơn."} +{"idx": 1870023, "en": "BACKGROUND Health behavior change can be modelled in terms of stages, and outcomes of transitions between stages can be categorized into progression, regression, and stagnation. Based on the Health Action Process Approach this study tested whether changes in social-cognitive variables are associated with transitions between stages regarding physical activity (PA) and fruit and vegetable intake (FVI). METHODS N = 132 participants (M = 50.86 years, SD = 13.17, 61.4% women) were assessed at baseline and 8 weeks later. Data were analysed using multivariate analyses of variance (MANOVA) and post-hoc comparisons. RESULTS Changes in motivational self-efficacy (η2  = 0.081), maintenance self-efficacy (η2  = 0.119), and recovery self-efficacy (η2  = 0.049) as well as positive outcome expectancies (η2  = 0.070), negative outcome expectancies (η2  = 0.055), and coping planning (η2  = 0.065) were associated with FVI stage progression. For PA, changes were not associated with stage progression. CONCLUSION To facilitate behavior change effectively, at least for FVI, it is essential to consider underlying mechanisms such as several aspects of self-efficacy in performing the desired health behaviors, outcome expectations, and planning how to overcome barriers. Additionally, the adoption of a stage approach may be a useful starting point to develop stage-matched interventions.", "gemini": "BỐI CẢNH Thay đổi hành vi sức khỏe có thể được mô hình hóa theo từng giai đoạn và kết quả của quá trình chuyển đổi giữa các giai đoạn có thể được phân loại thành tiến triển, thoái triển và trì trệ. Dựa trên Phương pháp tiếp cận quá trình hành động vì sức khỏe, nghiên cứu này đã kiểm tra xem những thay đổi về các biến số nhận thức xã hội có liên quan đến quá trình chuyển đổi giữa các giai đoạn liên quan đến hoạt động thể chất (PA) và lượng trái cây và rau quả (FVI) hay không. PHƯƠNG PHÁP N = 132 người tham gia (M = 50,86 tuổi, SD = 13,17, 61,4% là phụ nữ) đã được đánh giá lúc ban đầu và 8 tuần sau đó. Dữ liệu được phân tích bằng phân tích phương sai đa biến (MANOVA) và các so sánh hậu kiểm. KẾT QUẢ Những thay đổi về hiệu quả tự lực thúc đẩy (η2  = 0,081), hiệu quả tự lực duy trì (η2  = 0,119) và hiệu quả tự lực phục hồi (η2  = 0,049) cũng như kỳ vọng kết quả tích cực (η2  = 0,070), kỳ vọng kết quả tiêu cực (η2  = 0,055) và kế hoạch đối phó (η2  = 0,065) có liên quan đến quá trình tiến triển giai đoạn FVI. Đối với PA, những thay đổi không liên quan đến quá trình tiến triển giai đoạn. KẾT LUẬN Để tạo điều kiện thuận lợi cho việc thay đổi hành vi một cách hiệu quả, ít nhất là đối với FVI, điều cần thiết là phải xem xét các cơ chế cơ bản như một số khía cạnh của hiệu quả tự lực trong việc thực hiện các hành vi sức khỏe mong muốn, kỳ vọng kết quả và lập kế hoạch để vượt qua rào cản. Ngoài ra, việc áp dụng phương pháp tiếp cận từng giai đoạn có thể là một điểm khởi đầu hữu ích để phát triển các biện pháp can thiệp phù hợp với từng giai đoạn."} +{"idx": 1870024, "en": "INTRODUCTION Reported incidence and prevalence rates of Myasthenia Gravis (MG) vary widely and are assumed to have increased over the last few decades. We conducted a nationwide register-based study on the current incidence and prevalence of MG and MG subgroups in Sweden. METHODS Data were acquired from four Swedish Health Registers in order to identify patients with MG. Incidence and prevalence rates were calculated for the years 2006-2016, using population numbers provided by Statistics Sweden. RESULTS In 2016, the incidence of MG in Sweden was 2.9 per 100,000 inhabitants (95% CI: 2.5-3.2/100,000) and the crude prevalence was 36.1 per 100,000 inhabitants (95% CI: 34.9-37.3). There was a significant increase in Myasthenia Gravis prevalence from 2006 to 2016. Prevalence rates of all MG subgroups but thymoma-associated MG increased over the same period of time. CONCLUSIONS The incidence and prevalence of Myasthenia Gravis have increased over time in Sweden, and the rates are high in comparison with other countries.", "gemini": "GIỚI THIỆU Tỷ lệ mắc và tỷ lệ lưu hành của bệnh nhược cơ (MG) được báo cáo rất khác nhau và được cho là đã tăng trong vài thập kỷ qua. Chúng tôi đã tiến hành một nghiên cứu dựa trên sổ đăng ký trên toàn quốc về tỷ lệ mắc và tỷ lệ lưu hành hiện tại của MG và các nhóm nhỏ MG ở Thụy Điển. PHƯƠNG PHÁP Dữ liệu được thu thập từ bốn Sổ đăng ký sức khỏe của Thụy Điển để xác định những bệnh nhân mắc MG. Tỷ lệ mắc và tỷ lệ lưu hành được tính cho các năm 2006-2016, sử dụng số liệu dân số do Cục thống kê Thụy Điển cung cấp. KẾT QUẢ Vào năm 2016, tỷ lệ mắc MG ở Thụy Điển là 2,9 trên 100.000 dân (KTC 95%: 2,5-3,2/100.000) và tỷ lệ lưu hành thô là 36,1 trên 100.000 dân (KTC 95%: 34,9-37,3). Có sự gia tăng đáng kể về tỷ lệ lưu hành của bệnh nhược cơ từ năm 2006 đến năm 2016. Tỷ lệ lưu hành của tất cả các nhóm nhỏ MG nhưng MG liên quan đến u tuyến ức đều tăng trong cùng thời gian. KẾT LUẬN Tỷ lệ mắc và tỷ lệ lưu hành của bệnh nhược cơ đã tăng theo thời gian ở Thụy Điển và tỷ lệ này cao hơn so với các quốc gia khác."} +{"idx": 1870025, "en": "Eukaryotic gene expression is tightly regulated during translation of mRNA to protein. Mis-regulation of translation can lead to aberrant proteins which accumulate in cancers and cause neurodegenerative diseases. Foundational studies on model genes established fundamental roles for mRNA 5' transcript leader (TL) sequences in controlling ribosome recruitment, scanning, and initiation. TL cis-regulatory elements and their corresponding trans-acting factors control cap-dependent initiation under unstressed conditions. Under stress, cap-dependent initiation is suppressed, and specific mRNA structures and sequences promote translation of stress-responsive transcripts to remodel the proteome. In this review, we summarize current knowledge of TL functions in translation initiation. We focus on insights from high-throughput analyses of ribosome occupancy, mRNA structure, RNA Binding Protein occupancy, and massively parallel reporter assays. These data-driven approaches, coupled with computational analyses and modeling, have paved the way for a comprehensive understanding of TL functions. Finally, we will discuss areas of future research on the roles of mRNA sequences and structures in translation. This article is categorized under: Translation > Translation Mechanisms RNA Evolution and Genomics > Computational Analyses of RNA RNA Structure and Dynamics > Influence of RNA Structure in Biological Systems.", "gemini": "Sự biểu hiện gen của sinh vật nhân chuẩn được điều hòa chặt chẽ trong quá trình dịch mRNA thành protein. Sự điều hòa sai lệch của quá trình dịch có thể dẫn đến các protein bất thường tích tụ trong ung thư và gây ra các bệnh thoái hóa thần kinh. Các nghiên cứu cơ bản về các gen mẫu đã thiết lập các vai trò cơ bản của trình tự đầu dẫn mRNA (TL) trong việc kiểm soát sự tuyển dụng ribosome, quét và khởi đầu. Các yếu tố điều hòa cis-TL và các yếu tố hoạt động tương ứng của chúng kiểm soát quá trình khởi đầu phụ thuộc mũ trong điều kiện không bị căng thẳng. Trong điều kiện căng thẳng, quá trình khởi đầu phụ thuộc mũ bị ức chế và các cấu trúc và trình tự mRNA cụ thể thúc đẩy quá trình dịch các bản sao đáp ứng với căng thẳng để tái tạo lại proteome. Trong bài đánh giá này, chúng tôi tóm tắt kiến thức hiện tại về các chức năng TL trong quá trình khởi đầu dịch. Chúng tôi tập trung vào những hiểu biết từ các phân tích thông lượng cao về mức độ chiếm giữ ribosome, cấu trúc mRNA, mức độ chiếm giữ protein liên kết RNA và các xét nghiệm báo cáo song song ồ ạt. Những cách tiếp cận dựa trên dữ liệu này, kết hợp với các phân tích và mô hình tính toán, đã mở đường cho sự hiểu biết toàn diện về các chức năng TL. Cuối cùng, chúng tôi sẽ thảo luận về các lĩnh vực nghiên cứu trong tương lai về vai trò của trình tự và cấu trúc mRNA trong quá trình dịch. Bài viết này được phân loại theo: Dịch > Cơ chế dịch Tiến hóa và di truyền học RNA > Phân tích tính toán RNA Cấu trúc và động lực học RNA > Ảnh hưởng của cấu trúc RNA trong các hệ thống sinh học."} +{"idx": 1870026, "en": "OBJECTIVE Propofol is one of the most commonly used intravenous drugs to induce and maintain general anesthesia. In vivo and in vitro studies have shown that propofol can affect neuronal growth, leading to apoptosis and impairing cognitive function. The Abelson nonreceptor tyrosine kinase (c-Abl) is associated with both neuritic plaques and neurofibrillary tangles in the brains of patients with Alzheimer's disease and other neurodegenerative diseases. This study aimed to explore the effect of propofol on apoptosis and neurocognition through its regulation of c-Abl expression in vivo and in vitro. MATERIALS AND METHODS In this study, primary hippocampal neurons were cultured and exposed to propofol at different concentrations. Protein expression was measured by Western blotting and coimmunoprecipitation. The c-Abl transcription level was verified by fluorescence quantitative PCR. Reactive oxygen species (ROS) levels were detected by flow cytometry. In addition, an animal experiment was conducted to assess neuronal apoptosis by immunofluorescence staining for caspase-3 and to evaluate behavioral changes by the Morris water maze (MWM) test. RESULTS The in vitro experiment showed that propofol significantly decreased c-Abl expression and ROS levels. In addition, propofol has no cytotoxic effect and does not affect cell activity. Moreover, in the animal experiment, intraperitoneal injection of 50 mg/kg propofol for 5 days obviously decreased the expression of c-Abl in the neonatal rat brain (p < .05) but did not significantly increase the number of caspase-3-positive cells. Propofol treatment did not significantly reduce the number of platform crossings (p > .05) or prolong the escape latency of neonatal rats (p > .05) in the MWM test. CONCLUSIONS The present data suggest that reduced expression of this nonreceptor tyrosine kinase through consecutive daily administration of propofol did not impair learning or memory function in neonatal rats.", "gemini": "MỤC TIÊU Propofol là một trong những loại thuốc tiêm tĩnh mạch được sử dụng phổ biến nhất để gây mê toàn thân và duy trì gây mê. Các nghiên cứu trong ống nghiệm và trong cơ thể sống đã chỉ ra rằng propofol có thể ảnh hưởng đến sự phát triển của tế bào thần kinh, dẫn đến chết tế bào theo chương trình và làm suy yếu chức năng nhận thức. Tyrosine kinase không phải thụ thể Abelson (c-Abl) có liên quan đến cả mảng neuritic và các đám rối sợi thần kinh trong não của bệnh nhân mắc bệnh Alzheimer và các bệnh thoái hóa thần kinh khác. Nghiên cứu này nhằm mục đích khám phá tác dụng của propofol đối với quá trình chết tế bào theo chương trình và nhận thức thần kinh thông qua việc điều hòa biểu hiện c-Abl trong cơ thể sống và trong ống nghiệm. VẬT LIỆU VÀ PHƯƠNG PHÁP Trong nghiên cứu này, các tế bào thần kinh đồi hải mã sơ cấp được nuôi cấy và tiếp xúc với propofol ở các nồng độ khác nhau. Biểu hiện protein được đo bằng phương pháp điện di Western và kết tủa miễn dịch. Mức phiên mã c-Abl được xác minh bằng PCR định lượng huỳnh quang. Nồng độ các loài oxy phản ứng (ROS) được phát hiện bằng phương pháp đo lưu lượng tế bào. Ngoài ra, một thí nghiệm trên động vật đã được tiến hành để đánh giá quá trình chết tế bào theo chương trình của tế bào thần kinh bằng cách nhuộm miễn dịch huỳnh quang đối với caspase-3 và để đánh giá những thay đổi về hành vi bằng thử nghiệm mê cung Morris (MWM). KẾT QUẢ Thí nghiệm trong ống nghiệm cho thấy propofol làm giảm đáng kể biểu hiện c-Abl và mức ROS. Ngoài ra, propofol không có tác dụng gây độc tế bào và không ảnh hưởng đến hoạt động của tế bào. Hơn nữa, trong thí nghiệm trên động vật, tiêm phúc mạc 50 mg/kg propofol trong 5 ngày rõ ràng làm giảm biểu hiện của c-Abl trong não chuột sơ sinh (p < 0,05) nhưng không làm tăng đáng kể số lượng tế bào dương tính với caspase-3. Điều trị bằng propofol không làm giảm đáng kể số lần vượt bục (p > 0,05) hoặc kéo dài thời gian thoát khỏi mê cung của chuột sơ sinh (p > 0,05) trong thử nghiệm MWM. KẾT LUẬN Dữ liệu hiện tại cho thấy việc giảm biểu hiện của tyrosine kinase không phải thụ thể này thông qua việc dùng propofol hàng ngày liên tiếp không làm suy yếu chức năng học tập hoặc trí nhớ ở chuột sơ sinh."} +{"idx": 1870027, "en": "Excitotoxicity is a cellular phenomenon that comprises the consequences of toxic actions of excitatory neurotransmitters, such as glutamate. This process is usually related to overproduction of reactive oxygen species (ROS) and ammonia (NH4 + ) toxicity. Platinum nanoparticle (Pt-NP)-based microreactors able to degrade hydrogen peroxide (H2 O2 ) and NH4 + , are previously described as a novel therapeutical approach against excitotoxicity, conferring protection to neuroblasts. Now, it is demonstrated that these microreactors are compatible with rat primary cortical neurons, show high levels of neuronal membrane interaction, and are able to improve cell survival and neuronal activity when neurons are exposed to H2 O2 or NH4 + . Additionally, more complex microreactors are assembled, including enzyme-loaded liposomes containing glutamate dehydrogenase and glutathione reductase, in addition to Pt-NP. The in vitro activity of these microreactors is characterized and they are compared to the Pt-NP-based microreactors in terms of biological activity, concluding that they enhance cell viability similarly or more extensively than the latter. Extracellular electrophysiological recordings demonstrate that these microreactors rescue neuronal functionality lost upon incubation with H2 O2 or NH4 + . This study provides more evidence for the potential application of these microreactors in a biomedical context with more complex cellular environments.", "gemini": "Kích thích độc tế bào là hiện tượng tế bào bao gồm hậu quả của các tác động độc hại của các chất dẫn truyền thần kinh kích thích, chẳng hạn như glutamate. Quá trình này thường liên quan đến việc sản xuất quá mức các loài oxy phản ứng (ROS) và độc tính amoniac (NH4 +). Các lò phản ứng vi mô dựa trên hạt nano bạch kim (Pt-NP) có khả năng phân hủy hydro peroxide (H2 O2) và NH4 +, trước đây được mô tả như một phương pháp điều trị mới chống lại kích thích độc tế bào, mang lại sự bảo vệ cho các tế bào thần kinh. Bây giờ, người ta chứng minh rằng các lò phản ứng vi mô này tương thích với các tế bào thần kinh vỏ não nguyên phát của chuột, cho thấy mức độ tương tác màng tế bào thần kinh cao và có khả năng cải thiện sự sống còn của tế bào và hoạt động của tế bào thần kinh khi các tế bào thần kinh tiếp xúc với H2 O2 hoặc NH4 +. Ngoài ra, các lò phản ứng vi mô phức tạp hơn được lắp ráp, bao gồm các liposome chứa đầy enzyme có chứa glutamate dehydrogenase và glutathione reductase, ngoài Pt-NP. Hoạt động trong ống nghiệm của các lò phản ứng vi mô này được đặc trưng và chúng được so sánh với các lò phản ứng vi mô dựa trên Pt-NP về hoạt tính sinh học, kết luận rằng chúng tăng cường khả năng sống của tế bào tương tự hoặc rộng hơn so với các lò phản ứng vi mô sau. Các bản ghi điện sinh lý ngoại bào chứng minh rằng các lò phản ứng vi mô này cứu vãn chức năng của tế bào thần kinh bị mất khi ủ với H2 O2 hoặc NH4 +. Nghiên cứu này cung cấp thêm bằng chứng cho ứng dụng tiềm năng của các lò phản ứng vi mô này trong bối cảnh y sinh học với môi trường tế bào phức tạp hơn."} +{"idx": 1870028, "en": "INTRODUCTION Ultrasound (US) plays a key role in the detection of testicular tumours. However, reliable characterisation of testicular tumours with US is difficult. The purpose of this study was to investigate the morphological patterns of testicular tumours as seen on modern US imaging and correlate these with histology. METHODS The imaging features of 50 testicular tumours were analysed and compared with histology. The US appearance was categorized into 15 distinct morphological patterns. RESULTS Patient's age ranged from 0.5 to 85 years. Of the 50 tumours in our series, 49 were malignant. Nearly half of the malignancies were seminomatous germ cell tumours (SGCTs). Tumours ranged in size from 10 to 130 mm with considerable overlap of size between tumours of different histological type. Even small (10 mm) tumours in our cohort were malignant. SGCTs demonstrated a narrower range of morphological appearances than non-seminomatous germ cell tumours (NSGCTs). Calcification was common in both SGCT and NSGCTs. Multicomponent cystic-solid appearance was only seen in NSGCTs. CONCLUSION The differentiation of testicular tumours with US continues to be challenging. In this paper, we have demonstrated the diverse morphological patterns of testicular neoplasms and have proposed the study of tumour morphological features as a promising research direction.", "gemini": "GIỚI THIỆU Siêu âm (US) đóng vai trò quan trọng trong việc phát hiện khối u tinh hoàn. Tuy nhiên, việc mô tả đáng tin cậy các khối u tinh hoàn bằng siêu âm là rất khó. Mục đích của nghiên cứu này là để tìm hiểu các mô hình hình thái của khối u tinh hoàn như được thấy trên hình ảnh siêu âm hiện đại và tương quan chúng với mô học. PHƯƠNG PHÁP Các đặc điểm hình ảnh của 50 khối u tinh hoàn đã được phân tích và so sánh với mô học. Hình ảnh siêu âm được phân loại thành 15 mô hình hình thái riêng biệt. KẾT QUẢ Độ tuổi của bệnh nhân từ 0,5 đến 85 tuổi. Trong số 50 khối u trong loạt nghiên cứu của chúng tôi, có 49 khối u là ác tính. Gần một nửa số khối u ác tính là khối u tế bào mầm tinh hoàn (SGCT). Kích thước khối u dao động từ 10 đến 130 mm với sự chồng chéo đáng kể về kích thước giữa các khối u có loại mô học khác nhau. Ngay cả những khối u nhỏ (10 mm) trong nhóm nghiên cứu của chúng tôi cũng là ác tính. SGCT cho thấy phạm vi hình thái hẹp hơn so với các khối u tế bào mầm không phải tinh hoàn (NSGCT). Vôi hóa phổ biến ở cả SGCT và NSGCT. Hình ảnh nang-rắn đa thành phần chỉ được thấy ở NSGCT. KẾT LUẬN Việc phân biệt các khối u tinh hoàn bằng siêu âm vẫn còn nhiều thách thức. Trong bài báo này, chúng tôi đã chứng minh các mô hình hình thái đa dạng của các khối u tân sinh tinh hoàn và đã đề xuất nghiên cứu các đặc điểm hình thái của khối u như một hướng nghiên cứu đầy hứa hẹn."} +{"idx": 1870029, "en": "Dehaloperoxidase (DHP) catalyzes detoxifying halophenols. It is a heme-containing enzyme using H2 O2 as the oxidant. Heme bleaching from the active site is of great concern. In addition, the interference of DHP by H2 O2 leads to the inactivation of the enzyme. To solve these two problems, DHP is coordinated to Zn (II) in PBS buffer to form a biomineralized composite (DHP&Zn-CP). DHP&Zn-CP was characterized by measuring SEM and confocal images, as well as energy dispersive X-ray spectrometry mapping. Fluorescence spectra demonstrated that DHP&Zn-CP can prevent heme bleaching. Two-dimensional FTIR spectra were measured, dynamically providing insight into the structural change of DHP along the coordination process. Raman spectra were performed to analyze the structural change. The optical spectra confirmed that the forming of DHP&Zn-CP had a little effect on the structures of DHP. For the dehalogenation of 2,4,6-trichlorophenol, DHP&Zn-CP can tolerate the presence of H2 O2 and is resistant to the interference by H2 O2 . The catalytic efficiency of DHP&Zn-CP is much higher than that of free DHP.", "gemini": "Dehaloperoxidase (DHP) xúc tác quá trình giải độc halophenol. Đây là một enzyme chứa heme sử dụng H2 O2 làm chất oxy hóa. Việc tẩy trắng heme khỏi vị trí hoạt động là mối quan tâm lớn. Ngoài ra, sự can thiệp của DHP bởi H2 O2 dẫn đến việc bất hoạt enzyme. Để giải quyết hai vấn đề này, DHP được phối hợp với Zn (II) trong dung dịch đệm PBS để tạo thành một hợp chất khoáng sinh học (DHP & Zn-CP). DHP & Zn-CP được đặc trưng bằng cách đo hình ảnh SEM và ảnh cộng hưởng, cũng như ánh xạ phổ X-quang phân tán năng lượng. Phổ huỳnh quang chứng minh rằng DHP & Zn-CP có thể ngăn ngừa quá trình tẩy trắng heme. Phổ FTIR hai chiều đã được đo, cung cấp cái nhìn sâu sắc về sự thay đổi cấu trúc của DHP trong suốt quá trình phối hợp. Phổ Raman đã được thực hiện để phân tích sự thay đổi cấu trúc. Phổ quang học xác nhận rằng sự hình thành của DHP & Zn-CP có ảnh hưởng nhỏ đến cấu trúc của DHP. Đối với quá trình khử halogen của 2,4,6-trichlorophenol, DHP & Zn-CP có thể chịu được sự hiện diện của H2 O2 và có khả năng chống lại sự can thiệp của H2 O2. Hiệu quả xúc tác của DHP & Zn-CP cao hơn nhiều so với DHP tự do."} +{"idx": 1870030, "en": "OBJECTIVES This study aimed to compare the prognosis according to age, genotype or human papillomavirus (HPV) variant in patients with recurrent respiratory papillomatosis (RRP). DESIGN Non-concurrent cohort. PARTICIPANTS Forty one patients with RRP. SETTING Tertiary referral hospital. MAIN OUTCOME MEASURES Disease severity was defined by the number of surgeries performed, and Derkay score at surgeries, obtained from medical records. HPV was detected and genotyped, and HPV-6 variants were also assessed. RESULTS Fifteen (36.58%) individuals belonged to the juvenile RRP group (JoRRP, less than 18 years), while 26 patients (63.41%) were allocated at the adult group (AoRRP, equal or more than 18 years). JoRRP patients needed, in average, a higher number of surgeries to control the disease than AoRRP patients (mean difference: 3.36). Also, JoRRP patients showed a higher Derkay score at each surgery (mean difference: 3.76). There was no significant difference in the number of surgeries when we compared patients infected with HPV-6 or HPV-11, neither in accordance to HPV-6 variants. Patients with HPV-11 presented a higher mean Derkay score at surgery than those with HPV-6 (mean difference: 4.39); when co-variated by age, we observed that this difference occurred only among JoRRP patients (mean difference: 6.15). CONCLUSIONS Age of onset of RRP has an important impact on number of surgeries to control disease. Patients with JoRRP and HPV-11 tend to present worse Derkay score at each surgery. HPV genotype among adults and HPV-6 variants had no impact on the outcome of the disease.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm so sánh tiên lượng theo tuổi, kiểu gen hoặc biến thể vi-rút papilloma ở người (HPV) ở những bệnh nhân bị u nhú thanh quản tái phát (RRP). THIẾT KẾ Đối chứng không đồng thời. NGƯỜI THAM GIA Bốn mươi mốt bệnh nhân bị RRP. BỐI CẢNH Bệnh viện chuyên khoa tuyến ba. CÁC BIỆN PHÁP ĐO LƯỜNG KẾT QUẢ CHÍNH Mức độ nghiêm trọng của bệnh được xác định bằng số lần phẫu thuật thực hiện và điểm Derkay tại các lần phẫu thuật, lấy từ hồ sơ bệnh án. HPV đã được phát hiện và phân loại kiểu gen, và các biến thể HPV-6 cũng được đánh giá. KẾT QUẢ Mười lăm (36,58%) cá nhân thuộc nhóm RRP ở trẻ em (JoRRP, dưới 18 tuổi), trong khi 26 bệnh nhân (63,41%) được phân vào nhóm người lớn (AoRRP, từ 18 tuổi trở lên). Bệnh nhân JoRRP cần trung bình số lần phẫu thuật cao hơn để kiểm soát bệnh so với bệnh nhân AoRRP (chênh lệch trung bình: 3,36). Ngoài ra, bệnh nhân JoRRP có điểm Derkay cao hơn ở mỗi lần phẫu thuật (chênh lệch trung bình: 3,76). Không có sự khác biệt đáng kể về số lần phẫu thuật khi chúng tôi so sánh những bệnh nhân bị nhiễm HPV-6 hoặc HPV-11, cũng không giống như các biến thể HPV-6. Bệnh nhân nhiễm HPV-11 có điểm Derkay trung bình cao hơn khi phẫu thuật so với những bệnh nhân nhiễm HPV-6 (chênh lệch trung bình: 4,39); khi thay đổi theo tuổi, chúng tôi thấy rằng sự khác biệt này chỉ xảy ra ở những bệnh nhân JoRRP (chênh lệch trung bình: 6,15). KẾT LUẬN Tuổi khởi phát RRP có tác động quan trọng đến số lần phẫu thuật để kiểm soát bệnh. Bệnh nhân JoRRP và HPV-11 có xu hướng có điểm Derkay tệ hơn ở mỗi lần phẫu thuật. Kiểu gen HPV ở người lớn và các biến thể HPV-6 không ảnh hưởng đến kết cục của bệnh."} +{"idx": 1870031, "en": "As a milestone in soft and hard tissue engineering, a precise control over the micropatterns of scaffolds has lightened new opportunities for the recapitulation of native body organs through three dimentional (3D) bioprinting approaches. Well-printable bioinks are prerequisites for the bioprinting of tissues/organs where hydrogels play a critical role. Despite the outstanding developments in 3D engineered microstructures, current printer devices suffer from the risk of exposing loaded living agents to mechanical (nozzle-based) and thermal (nozzle-free) stresses. Thus, tuning the rheological, physical, and mechanical properties of hydrogels is a promising solution to address these issues. The relationship between the mechanical characteristics of hydrogels and their printability is important to control printing quality and fidelity. Recent developments in defining this relationship have highlighted the decisive role of main additive manufacturing strategies. These strategies are applied to enhance the printing quality of scaffolds and determine the nurture of cellular morphology. In this regard, it is beneficial to use external and internal stabilization, photocurable biopolymers, and cooling substrates containing the printed scaffolds. The objective of this study is to review cutting-edge developments in hydrogel-type bioinks and discuss the optimum simulation of the zonal stratification in osteochondral and cartilage units.", "gemini": "Là một cột mốc trong kỹ thuật mô mềm và mô cứng, việc kiểm soát chính xác các vi mô của giá đỡ đã mở ra những cơ hội mới để tái tạo các cơ quan cơ thể bản địa thông qua các phương pháp in sinh học ba chiều (3D). Các loại mực sinh học có thể in tốt là điều kiện tiên quyết để in sinh học các mô/cơ quan mà tại đó các loại hydrogel đóng vai trò quan trọng. Mặc dù có những tiến bộ vượt bậc trong các cấu trúc vi được thiết kế theo kỹ thuật 3D, nhưng các thiết bị máy in hiện tại vẫn có nguy cơ làm lộ các tác nhân sống đã tải cho các ứng suất cơ học (dựa trên vòi phun) và nhiệt (không có vòi phun). Do đó, điều chỉnh các đặc tính lưu biến, vật lý và cơ học của hydrogel là một giải pháp đầy hứa hẹn để giải quyết những vấn đề này. Mối quan hệ giữa các đặc tính cơ học của hydrogel và khả năng in của chúng rất quan trọng để kiểm soát chất lượng và độ trung thực của bản in. Những phát triển gần đây trong việc xác định mối quan hệ này đã làm nổi bật vai trò quyết định của các chiến lược sản xuất phụ gia chính. Các chiến lược này được áp dụng để nâng cao chất lượng in của giá đỡ và xác định sự nuôi dưỡng hình thái tế bào. Về vấn đề này, việc sử dụng chất ổn định bên ngoài và bên trong, các chất sinh học quang trùng hợp và các chất nền làm mát chứa các giá đỡ đã in là có lợi. Mục tiêu của nghiên cứu này là xem xét các phát triển tiên tiến trong các loại mực sinh học dạng hydrogel và thảo luận về mô phỏng tối ưu sự phân tầng theo vùng ở các đơn vị xương sụn và sụn."} +{"idx": 1870032, "en": "INTRODUCTION As adolescents transition from childhood to adulthood, they experience major physical, social and psychological changes, and are at heightened risk for developing mental health conditions and engaging in health-related risk behaviours. For adolescents living with HIV (ALHIV), these risks may be even more pronounced. Research shows that this population may face additional mental health challenges related to the biological impact of the disease and its treatment, the psychosocial burdens of living with HIV and HIV-related social and environmental stressors. DISCUSSION Psychosocial interventions delivered to adolescents can promote positive mental health, prevent mental health problems and strengthen young people's capacity to navigate challenges and protect themselves from risk. It is likely that these interventions can also benefit at-risk populations, such as ALHIV, yet there is little research on this. There is an urgent need for more research evaluating the effects of interventions designed to improve the mental health of ALHIV. We highlight four priorities moving forward. These include: generating more evidence about preventive mental health interventions for ALHIV; including mental health outcomes in research on psychosocial interventions for ALHIV; conducting intervention research that is sensitive to differences among ALHIV populations and involving adolescents in intervention design and testing. CONCLUSIONS More robust research on promotive and preventive mental health interventions is needed for ALHIV. Programmes should be informed by adolescent priorities and preferences and responsive to the specific needs of these groups.", "gemini": "GIỚI THIỆU Khi thanh thiếu niên chuyển từ thời thơ ấu sang tuổi trưởng thành, các em sẽ trải qua những thay đổi lớn về thể chất, xã hội và tâm lý, và có nguy cơ cao mắc các bệnh về sức khỏe tâm thần và tham gia vào các hành vi có nguy cơ liên quan đến sức khỏe. Đối với thanh thiếu niên sống chung với HIV (ALHIV), những rủi ro này thậm chí còn rõ rệt hơn. Nghiên cứu cho thấy nhóm dân số này có thể phải đối mặt với các vấn đề sức khỏe tâm thần khác liên quan đến tác động sinh học của bệnh và cách điều trị, gánh nặng tâm lý xã hội khi sống chung với HIV và các yếu tố gây căng thẳng về mặt xã hội và môi trường liên quan đến HIV. THẢO LUẬN Các can thiệp tâm lý xã hội dành cho thanh thiếu niên có thể thúc đẩy sức khỏe tâm thần tích cực, ngăn ngừa các vấn đề sức khỏe tâm thần và tăng cường khả năng của những người trẻ tuổi trong việc vượt qua những thách thức và tự bảo vệ mình khỏi nguy cơ. Có khả năng là những can thiệp này cũng có thể có lợi cho các nhóm dân số có nguy cơ, chẳng hạn như ALHIV, nhưng vẫn còn ít nghiên cứu về vấn đề này. Cần có thêm nhiều nghiên cứu đánh giá hiệu quả của các can thiệp được thiết kế để cải thiện sức khỏe tâm thần của ALHIV. Chúng tôi nêu bật bốn ưu tiên để tiến lên phía trước. Những điều này bao gồm: tạo ra nhiều bằng chứng hơn về các can thiệp sức khỏe tâm thần phòng ngừa cho ALHIV; đưa các kết quả về sức khỏe tâm thần vào nghiên cứu về các can thiệp tâm lý xã hội cho ALHIV; tiến hành nghiên cứu can thiệp nhạy cảm với sự khác biệt giữa các nhóm dân số ALHIV và liên quan đến thanh thiếu niên trong thiết kế và thử nghiệm can thiệp. KẾT LUẬN Cần có nhiều nghiên cứu sâu rộng hơn về các can thiệp sức khỏe tâm thần thúc đẩy và phòng ngừa cho ALHIV. Các chương trình nên được thông báo bởi các ưu tiên và sở thích của thanh thiếu niên và đáp ứng các nhu cầu cụ thể của các nhóm này."} +{"idx": 1870033, "en": "INTRODUCTION Low rates of viral suppression among adolescents living with HIV (ALHIV) indicate that more effective support is urgently required at scale. The provision of peer support has generated considerable enthusiasm because it has the potential to ameliorate the complex social and relational challenges which underpin suboptimal adherence. Little is known about the impact on young peer supporters themselves, which is the focus of this paper. METHODS We present qualitative findings from the Zvandiri trial investigating the impact of a peer support intervention on the viral load for beneficiaries (ALHIV, aged 13 to 19 years) in Zimbabwe. The Zvandiri peer supporters aged 18 to 24 years, known as community adolescent treatment supporters (CATS), are themselves living with HIV. Individual in-depth interviews were conducted in late 2018 with 17 CATS exploring their experiences of delivering peer support and their own support needs. Interviews were analysed iteratively using thematic analysis. RESULTS The CATS reported that being peer supporters improved their own adherence behaviour and contributed to an improved sense of self-worth. The social connections between the CATS were a source of comfort and enabled them to develop skills to manage the challenging aspects of their work. Two substantial challenges were identified. First, their work may reveal their HIV status. Second, managing the emotional labour of this caring work; given how commonly the complexity of the beneficiaries' needs mirrored the circumstances of their own difficult lives. Both challenges were ameliorated by the support the CATS provided to each other and ongoing supervision from the adult mentor. There was variation in whether they felt their roles were appropriately valued through the remuneration they received and within the health system. There was a consensus that their experience meant that they would graduate from being a CATS with transferable skills that could enhance their employability. CONCLUSIONS Their experiences illustrate the advantages and opportunities of being a CATS. To minimize potential harms, it is vital to ensure that they feel valued in their role, which can be demonstrated by the provision of appropriate remuneration, recognition and respect, and that there is continued investment in ongoing support through ongoing training and mentoring.", "gemini": "GIỚI THIỆU Tỷ lệ ức chế vi-rút thấp ở thanh thiếu niên nhiễm HIV (ALHIV) chỉ ra rằng cần có sự hỗ trợ hiệu quả hơn ở quy mô lớn. Việc cung cấp hỗ trợ ngang hàng đã tạo nên sự nhiệt tình đáng kể vì nó có khả năng cải thiện các thách thức phức tạp về mặt xã hội và quan hệ vốn là nền tảng của việc tuân thủ không tối ưu. Người ta biết rất ít về tác động đối với chính những người hỗ trợ ngang hàng trẻ tuổi, đó là trọng tâm của bài báo này. PHƯƠNG PHÁP Chúng tôi trình bày những phát hiện định tính từ thử nghiệm Zvandiri nhằm điều tra tác động của can thiệp hỗ trợ ngang hàng đối với tải lượng vi-rút ở những người thụ hưởng (ALHIV, từ 13 đến 19 tuổi) tại Zimbabwe. Những người hỗ trợ ngang hàng Zvandiri từ 18 đến 24 tuổi, được gọi là những người hỗ trợ điều trị thanh thiếu niên cộng đồng (CATS), bản thân họ cũng đang sống chung với HIV. Các cuộc phỏng vấn chuyên sâu riêng lẻ đã được thực hiện vào cuối năm 2018 với 17 CATS để khám phá kinh nghiệm của họ về việc cung cấp hỗ trợ ngang hàng và nhu cầu hỗ trợ của chính họ. Các cuộc phỏng vấn đã được phân tích theo từng giai đoạn bằng cách sử dụng phân tích chủ đề. KẾT QUẢ Những người CATS báo cáo rằng việc trở thành người hỗ trợ ngang hàng đã cải thiện hành vi tuân thủ của chính họ và góp phần nâng cao ý thức về giá trị bản thân. Các kết nối xã hội giữa những người CATS là nguồn an ủi và giúp họ phát triển các kỹ năng để quản lý những khía cạnh đầy thách thức trong công việc của mình. Hai thách thức đáng kể đã được xác định. Đầu tiên, công việc của họ có thể tiết lộ tình trạng nhiễm HIV của họ. Thứ hai, quản lý công việc cảm xúc của công việc chăm sóc này; xét đến việc nhu cầu phức tạp của những người thụ hưởng thường phản ánh hoàn cảnh khó khăn trong cuộc sống của chính họ như thế nào. Cả hai thách thức đều được giảm bớt nhờ sự hỗ trợ mà những người CATS dành cho nhau và sự giám sát liên tục từ người cố vấn là người lớn. Có sự khác biệt về việc họ có cảm thấy vai trò của mình được đánh giá đúng thông qua khoản thù lao họ nhận được hay không và trong hệ thống y tế. Có một sự đồng thuận rằng kinh nghiệm của họ có nghĩa là họ sẽ tốt nghiệp khỏi việc trở thành CATS với các kỹ năng có thể chuyển giao có thể nâng cao khả năng kiếm việc của họ. KẾT LUẬN Kinh nghiệm của họ minh họa những lợi thế và cơ hội khi trở thành CATS. Để giảm thiểu những tác hại tiềm ẩn, điều quan trọng là phải đảm bảo rằng họ cảm thấy được coi trọng trong vai trò của mình, điều này có thể được chứng minh bằng cách cung cấp mức thù lao, sự công nhận và tôn trọng thích hợp, và tiếp tục đầu tư vào sự hỗ trợ liên tục thông qua đào tạo và cố vấn liên tục."} +{"idx": 1870034, "en": "OBJECTIVE Rheumatoid arthritis (RA) is associated with increased atherosclerotic cardiovascular disease (ASCVD). General population cohorts have shown African American individuals to have greater and Hispanic Americans to have lower cardiovascular disease prevalence when compared with non-Hispanic white individuals; however, the reasons for these findings are not clear. This systematic review seeks to describe the incidence and prevalence of ASCVD stratified by race/ethnicity within the US RA population. METHODS MEDLINE, Embase, and Cochrane databases were searched for studies that reported incidence or prevalence of ASCVD (including, but not limited to, fatal and nonfatal stroke, myocardial infarction, and cardiovascular death) in those with RA. Abstracts and full texts were screened separately for inclusion by two reviewers, with a third reviewer to resolve discrepancies. RESULTS We screened 2625 abstracts and fully reviewed 138 manuscripts. Twenty-one were included that cited at a minimum the percentage of non-Hispanic whites in their population. No publication meeting entry criteria initially stratified ASCVD by race/ethnicity. The average prevalent ASCVD in RA is 46.9% (95% CI: 46.8-47) (range of prevalent ASCVD: 30%-47%). The average incident ASCVD is 8.2% (95% CI: 8.14-8.25) (range of incident ASCVD 1%-46%). CONCLUSION In this systematic review, we found a paucity of data on racially/ethnically diverse RA patients and ASCVD outcomes. Future studies should report the prevalence of ASCVD in various races/ethnicities with RA in the United States. These data would help inform clinicians on how best to manage cardiovascular disease risk in RA.", "gemini": "MỤC TIÊU Viêm khớp dạng thấp (RA) có liên quan đến tăng nguy cơ mắc bệnh tim mạch xơ vữa động mạch (ASCVD). Các nhóm dân số nói chung đã chỉ ra rằng những người Mỹ gốc Phi có tỷ lệ mắc bệnh tim mạch cao hơn và người Mỹ gốc Tây Ban Nha có tỷ lệ mắc bệnh tim mạch thấp hơn khi so sánh với những người da trắng không phải gốc Tây Ban Nha; tuy nhiên, lý do cho những phát hiện này vẫn chưa rõ ràng. Đánh giá có hệ thống này nhằm mô tả tỷ lệ mắc và tỷ lệ lưu hành của ASCVD được phân tầng theo chủng tộc/dân tộc trong quần thể RA ở Hoa Kỳ. PHƯƠNG PHÁP Các cơ sở dữ liệu MEDLINE, Embase và Cochrane đã được tìm kiếm các nghiên cứu báo cáo tỷ lệ mắc hoặc tỷ lệ lưu hành của ASCVD (bao gồm, nhưng không giới hạn ở, đột quỵ tử vong và không tử vong, nhồi máu cơ tim và tử vong do tim mạch) ở những người mắc RA. Tóm tắt và toàn văn được sàng lọc riêng để đưa vào bởi hai người đánh giá, với người đánh giá thứ ba để giải quyết những điểm không nhất quán. KẾT QUẢ Chúng tôi đã sàng lọc 2625 bản tóm tắt và xem xét đầy đủ 138 bản thảo. Hai mươi mốt bản được đưa vào, trong đó ít nhất là tỷ lệ người da trắng không phải gốc Tây Ban Nha trong dân số của họ. Không có ấn phẩm nào đáp ứng tiêu chí nhập cảnh ban đầu được phân tầng ASCVD theo chủng tộc/dân tộc. Tỷ lệ lưu hành trung bình của ASCVD ở RA là 46,9% (95% CI: 46,8-47) (phạm vi ASCVD lưu hành: 30% -47%). Tỷ lệ mắc ASCVD trung bình là 8,2% (95% CI: 8,14-8,25) (phạm vi ASCVD mắc phải 1% -46%). KẾT LUẬN Trong đánh giá có hệ thống này, chúng tôi thấy rằng dữ liệu về bệnh nhân RA đa dạng về chủng tộc/dân tộc và kết quả ASCVD còn ít. Các nghiên cứu trong tương lai nên báo cáo tỷ lệ lưu hành của ASCVD ở các chủng tộc/dân tộc khác nhau mắc RA tại Hoa Kỳ. Những dữ liệu này sẽ giúp các bác sĩ lâm sàng biết cách tốt nhất để quản lý nguy cơ mắc bệnh tim mạch ở RA."} +{"idx": 1870035, "en": "AIMS Few investigations have been conducted to identify genetic determinants of common, polygenetic forms of heart failure (HF), and only a limited number of these genetic associations have been validated by multiple groups. METHODS AND RESULTS We performed a case-control study to further investigate the potential impact of 14 previously reported candidate genes on the risk of HF and specific HF sub-types. We also performed an exploratory genome-wide study. We included 799 patients with HF and 1529 controls. After adjusting for age, sex, and genetic ancestry, we found that the C allele of rs2234962 in BAG3 was associated with a decreased risk of idiopathic dilated cardiomyopathy (odds ratio 0.42, 95% confidence interval 0.25-0.68, P = 0.0005), consistent with a previous report. No association for the other primary variants or exploratory genome-wide study was found. CONCLUSIONS Our findings provide independent replication for the association between a common coding variant (rs2234962) in BAG3 and the risk of idiopathic dilated cardiomyopathy.", "gemini": "MỤC ĐÍCH Ít có cuộc điều tra nào được tiến hành để xác định các yếu tố quyết định di truyền của các dạng suy tim (HF) phổ biến, đa gen và chỉ một số ít các liên kết di truyền này đã được nhiều nhóm xác nhận. PHƯƠNG PHÁP VÀ KẾT QUẢ Chúng tôi đã thực hiện một nghiên cứu ca-đối chứng để điều tra sâu hơn tác động tiềm tàng của 14 gen ứng viên đã được báo cáo trước đó đối với nguy cơ mắc HF và các phân nhóm HF cụ thể. Chúng tôi cũng đã thực hiện một nghiên cứu toàn bộ hệ gen mang tính thăm dò. Chúng tôi đã đưa vào 799 bệnh nhân mắc HF và 1529 đối chứng. Sau khi điều chỉnh theo tuổi, giới tính và nguồn gốc di truyền, chúng tôi thấy rằng alen C của rs2234962 trong BAG3 có liên quan đến giảm nguy cơ mắc bệnh cơ tim giãn nở vô căn (tỷ lệ cược 0,42, khoảng tin cậy 95% 0,25-0,68, P = 0,0005), phù hợp với một báo cáo trước đó. Không tìm thấy mối liên quan nào đối với các biến thể chính khác hoặc nghiên cứu toàn bộ hệ gen mang tính thăm dò. KẾT LUẬN Những phát hiện của chúng tôi cung cấp sự sao chép độc lập cho mối liên quan giữa một biến thể mã hóa phổ biến (rs2234962) trong BAG3 và nguy cơ mắc bệnh cơ tim giãn nở vô căn."} +{"idx": 1870036, "en": "OBJECTIVE As a result of the pandemic of COVID-19, the public have been experiencing psychological distress. However, the prevalence of psychological distress during the COVID-19 pandemic remains unknown. Our objective was to evaluate the prevalence of psychological distress during COVID-19 outbreak and their risk factors, especially their internal paths and causality. METHODS A nationwide cross-sectional survey of the prevalence of mental disorders was conducted. We used Hospital Anxiety and Depression Scale (HADS) to estimate the prevalence of anxiety and depression. The internal paths and the causality of the psychological health were analyzed using a structural equation modeling (SEM) approach. RESULTS A total of 24,789 respondents completed the survey. We found that the overall prevalence of anxiety, depression, combination of anxiety, and depression were 51.6% (95% CI: 51.0-52.2), 47.5% (95% CI: 46.9-48.1), and 24.5% (95% CI: 24.0-25.0), respectively. The risk of psychological disorders in men was higher than that in women. The status of psychological health was different across different age groups, education levels, occupations, and income levels. The SEM analysis revealed that inadequate material supplies, low income, low education, lack of knowledge or confidence of the epidemic, and lack of exercise are major risk factors for psychological distress. CONCLUSIONS The evidence from this survey poses serious challenges related to the high prevalence of psychological distress, but also offers strategies to deal with the mental health problems caused by the COVID-19 pandemic.", "gemini": "MỤC TIÊU Do đại dịch COVID-19, công chúng đã trải qua những đau khổ về mặt tâm lý. Tuy nhiên, tỷ lệ đau khổ về mặt tâm lý trong đại dịch COVID-19 vẫn chưa được biết đến. Mục tiêu của chúng tôi là đánh giá tỷ lệ đau khổ về mặt tâm lý trong đợt bùng phát COVID-19 và các yếu tố rủi ro của họ, đặc biệt là các con đường nội tại và tính nhân quả của họ. PHƯƠNG PHÁP Đã tiến hành một cuộc khảo sát cắt ngang trên toàn quốc về tỷ lệ mắc các rối loạn tâm thần. Chúng tôi đã sử dụng Thang lo âu và trầm cảm của Bệnh viện (HADS) để ước tính tỷ lệ lo âu và trầm cảm. Các con đường nội tại và tính nhân quả của sức khỏe tâm lý đã được phân tích bằng cách sử dụng phương pháp lập mô hình phương trình cấu trúc (SEM). KẾT QUẢ Tổng cộng có 24.789 người trả lời đã hoàn thành cuộc khảo sát. Chúng tôi thấy rằng tỷ lệ lo âu, trầm cảm, kết hợp lo âu và trầm cảm lần lượt là 51,6% (95% CI: 51,0-52,2), 47,5% (95% CI: 46,9-48,1) và 24,5% (95% CI: 24,0-25,0). Nguy cơ mắc các rối loạn tâm lý ở nam giới cao hơn ở nữ giới. Tình trạng sức khỏe tâm lý khác nhau giữa các nhóm tuổi, trình độ học vấn, nghề nghiệp và mức thu nhập khác nhau. Phân tích SEM cho thấy nguồn cung vật chất không đầy đủ, thu nhập thấp, trình độ học vấn thấp, thiếu kiến thức hoặc sự tự tin về dịch bệnh và thiếu tập thể dục là những yếu tố nguy cơ chính gây ra đau khổ về mặt tâm lý. KẾT LUẬN Bằng chứng từ cuộc khảo sát này đặt ra những thách thức nghiêm trọng liên quan đến tỷ lệ đau khổ về mặt tâm lý cao, nhưng cũng đưa ra các chiến lược để đối phó với các vấn đề sức khỏe tâm thần do đại dịch COVID-19 gây ra."} +{"idx": 1870037, "en": "AIMS/INTRODUCTION This cohort study assessed the risk for bodyweight gain and development of glucose intolerance based on the frequency of consumption of balanced meals including grain, fish or meat and vegetables. MATERIALS AND METHODS The participants (8,573 men, 3,327 women) were employees of a company in Japan. A self-administered questionnaire was used to evaluate the frequency of balanced meal consumption. Bodyweight changes and the incidence of glucose intolerance (glycated hemoglobin >6.0%) during the 3-year follow-up period were determined through annual health examinations. RESULTS The mean bodyweight change over a period of 3 years was 0.78 kg for men and 0.84 kg for women. A lower frequency of balanced meals was associated with a higher bodyweight gain for men (P for trend = 0.004), but not for women. During the study, 464 men and 115 women developed glucose intolerance. Overall, the frequency of balanced meals was not associated with the risk of glucose intolerance in either sex. However, the interaction between the frequency of balanced meals and degree of obesity had a significant effect on the incidence of glucose intolerance in men (P = 0.005), with less frequent consumption of balanced meals being associated with a higher risk for glucose intolerance among men with a BMI ≥25.0 kg/m2 (P for trend = 0.007). CONCLUSIONS A higher frequency of balanced meals, including grain, fish or meat and vegetable dishes - important components of healthy Japanese food - was associated with a lower risk of glucose intolerance in obese men, but not in non-obese men and women.", "gemini": "MỤC ĐÍCH/GIỚI THIỆU Nghiên cứu đoàn hệ này đã đánh giá nguy cơ tăng cân và phát triển tình trạng không dung nạp glucose dựa trên tần suất tiêu thụ các bữa ăn cân bằng bao gồm ngũ cốc, cá hoặc thịt và rau. VẬT LIỆU VÀ PHƯƠNG PHÁP Những người tham gia (8.573 nam giới, 3.327 nữ giới) là nhân viên của một công ty tại Nhật Bản. Một bảng câu hỏi tự trả lời đã được sử dụng để đánh giá tần suất tiêu thụ bữa ăn cân bằng. Những thay đổi về cân nặng và tỷ lệ mắc bệnh không dung nạp glucose (hemoglobin glycosyl hóa> 6,0%) trong thời gian theo dõi 3 năm được xác định thông qua các kỳ kiểm tra sức khỏe hàng năm. KẾT QUẢ Sự thay đổi cân nặng trung bình trong khoảng thời gian 3 năm là 0,78 kg đối với nam giới và 0,84 kg đối với nữ giới. Tần suất ăn các bữa ăn cân bằng thấp hơn có liên quan đến việc tăng cân nhiều hơn ở nam giới (P theo xu hướng = 0,004), nhưng không phải ở nữ giới. Trong quá trình nghiên cứu, 464 nam giới và 115 nữ giới đã phát triển tình trạng không dung nạp glucose. Nhìn chung, tần suất ăn các bữa ăn cân bằng không liên quan đến nguy cơ không dung nạp glucose ở cả hai giới. Tuy nhiên, sự tương tác giữa tần suất ăn các bữa ăn cân bằng và mức độ béo phì có ảnh hưởng đáng kể đến tỷ lệ mắc bệnh không dung nạp glucose ở nam giới (P = 0,005), với việc tiêu thụ các bữa ăn cân bằng ít thường xuyên hơn có liên quan đến nguy cơ không dung nạp glucose cao hơn ở những người đàn ông có BMI ≥25,0 kg/m2 (P theo xu hướng = 0,007). KẾT LUẬN Tần suất ăn các bữa ăn cân bằng cao hơn, bao gồm các món ngũ cốc, cá hoặc thịt và rau - những thành phần quan trọng của thực phẩm Nhật Bản lành mạnh - có liên quan đến nguy cơ không dung nạp glucose thấp hơn ở những người đàn ông béo phì, nhưng không phải ở những người đàn ông và phụ nữ không béo phì."} +{"idx": 1870038, "en": "BACKGROUND Genetics is best dedicated to interpreting pathogenesis and revealing gene functions. The past decade has witnessed unprecedented progress in genetics, particularly in genome-wide identification of disorder variants through Genome-Wide Association Studies (GWAS) and Phenome-Wide Association Studies (PheWAS). However, it is still a great challenge to use GWAS/PheWAS-derived data to elucidate pathogenesis. METHODS In this study, we used HotNet2, a heat diffusion-based systems genetics algorithm, to calculate the networks for disease genes obtained from GWAS and PheWAS, with an attempt to get deeper insights into disease pathogenesis at a molecular level. RESULTS Through HotNet2 calculation, significant networks for 202 (for GWAS) and 167 (for PheWAS) types of diseases were identified and evaluated, respectively. The GWAS-derived disease networks exhibit a stronger biomedical relevance than PheWAS counterparts. Therefore, the GWAS-derived networks were used for pathogenesis interpretation by integrating the accumulated biomedical information. As a result, the pathogenesis for 64 diseases was elucidated in terms of mutation-caused abnormal transcriptional regulation, and 47 diseases were preliminarily interpreted in terms of mutation-caused varied protein-protein interactions. In addition, 3,802 genes (including 46 function-unknown genes) were assigned with new functions by disease network information, some of which were validated through mice gene knockout experiments. CONCLUSIONS Systems genetics algorithm HotNet2 can efficiently establish genotype-phenotype links at the level of biological networks. Compared with original GWAS/PheWAS results, HotNet2-calculated disease-gene associations have stronger biomedical significance, hence provide better interpretations for the pathogenesis of genome-wide variants, and offer new insights into gene functions as well. These results are also helpful in drug development.", "gemini": "BỐI CẢNH Di truyền học được dành riêng tốt nhất để giải thích cơ chế sinh bệnh và tiết lộ chức năng của gen. Thập kỷ qua đã chứng kiến sự tiến bộ chưa từng có trong lĩnh vực di truyền học, đặc biệt là trong việc xác định các biến thể rối loạn trên toàn bộ hệ gen thông qua Nghiên cứu liên kết toàn bộ hệ gen (GWAS) và Nghiên cứu liên kết toàn bộ hiện tượng (PheWAS). Tuy nhiên, việc sử dụng dữ liệu có nguồn gốc từ GWAS/PheWAS để làm sáng tỏ cơ chế sinh bệnh vẫn là một thách thức lớn. PHƯƠNG PHÁP Trong nghiên cứu này, chúng tôi đã sử dụng HotNet2, một thuật toán di truyền học hệ thống dựa trên sự khuếch tán nhiệt, để tính toán các mạng lưới cho các gen bệnh thu được từ GWAS và PheWAS, với mục đích có được hiểu biết sâu hơn về cơ chế sinh bệnh ở cấp độ phân tử. KẾT QUẢ Thông qua tính toán HotNet2, các mạng lưới quan trọng cho 202 (đối với GWAS) và 167 (đối với PheWAS) loại bệnh đã được xác định và đánh giá tương ứng. Các mạng lưới bệnh có nguồn gốc từ GWAS thể hiện tính liên quan y sinh mạnh hơn so với các mạng lưới đối tác PheWAS. Do đó, các mạng lưới có nguồn gốc từ GWAS đã được sử dụng để giải thích cơ chế sinh bệnh bằng cách tích hợp các thông tin y sinh đã tích lũy. Kết quả là, cơ chế sinh bệnh của 64 bệnh đã được làm sáng tỏ về mặt điều hòa phiên mã bất thường do đột biến gây ra và 47 bệnh đã được giải thích sơ bộ về mặt tương tác protein-protein khác nhau do đột biến gây ra. Ngoài ra, 3.802 gen (bao gồm 46 gen chưa rõ chức năng) đã được chỉ định các chức năng mới theo thông tin mạng lưới bệnh, một số trong số đó đã được xác nhận thông qua các thí nghiệm loại gen trên chuột. KẾT LUẬN Thuật toán di truyền học hệ thống HotNet2 có thể thiết lập hiệu quả các liên kết kiểu gen-kiểu hình ở cấp độ mạng lưới sinh học. So với kết quả GWAS/PheWAS ban đầu, các liên kết gen bệnh do HotNet2 tính toán có ý nghĩa y sinh mạnh mẽ hơn, do đó cung cấp giải thích tốt hơn cho cơ chế sinh bệnh của các biến thể trên toàn bộ hệ gen và cũng đưa ra những hiểu biết mới về chức năng của gen. Những kết quả này cũng hữu ích trong quá trình phát triển thuốc."} +{"idx": 1870039, "en": "BACKGROUND Adolescence and young adulthood are considered the peak age for the emergence of many psychiatric disorders, in particular major depressive disorder (MDD). Previous research has shown substantial heritability for MDD. In addition, the brain-derived neurotrophic factor (BDNF) gene is known to be associated with MDD. However, there has been no study conducting targeted sequencing of the BDNF gene in young MDD patients so far. METHOD To examine whether the BDNF gene is associated with the occurrence of MDD in young patients, we used targeted sequencing to detect the BDNF gene variants in 259 young Chinese Han people (105 MDD patients and 154 healthy subjects). RESULTS The BDNF variant rs4030470 was associated with MDD in young Chinese Han people (uncorrected p = 0.046), but this was no longer significant after applying FDR correction (p = 0.552, after FDR correction). We did not find any significant differences in genotype or haplotype frequencies between the case and control groups, and furthermore discovered no rare mutation variants any of the 259 subjects. CONCLUSION Our results do not support an association of the BDNF gene variants with MDD in young people in the Chinese Han population.", "gemini": "BỐI CẢNH Tuổi vị thành niên và thanh niên được coi là độ tuổi đỉnh cao để phát triển nhiều rối loạn tâm thần, đặc biệt là rối loạn trầm cảm nặng (MDD). Các nghiên cứu trước đây đã chỉ ra tính di truyền đáng kể đối với MDD. Ngoài ra, gen yếu tố dinh dưỡng thần kinh có nguồn gốc từ não (BDNF) được biết là có liên quan đến MDD. Tuy nhiên, cho đến nay vẫn chưa có nghiên cứu nào tiến hành giải trình tự mục tiêu của gen BDNF ở những bệnh nhân MDD trẻ tuổi. PHƯƠNG PHÁP Để kiểm tra xem gen BDNF có liên quan đến sự xuất hiện của MDD ở những bệnh nhân trẻ tuổi hay không, chúng tôi đã sử dụng giải trình tự mục tiêu để phát hiện các biến thể gen BDNF ở 259 người Hán trẻ tuổi (105 bệnh nhân MDD và 154 đối tượng khỏe mạnh). KẾT QUẢ Biến thể BDNF rs4030470 có liên quan đến MDD ở người Hán trẻ tuổi (p không hiệu chỉnh = 0,046), nhưng điều này không còn có ý nghĩa sau khi áp dụng hiệu chỉnh FDR (p = 0,552, sau hiệu chỉnh FDR). Chúng tôi không tìm thấy bất kỳ sự khác biệt đáng kể nào về tần số kiểu gen hoặc kiểu gen giữa các nhóm trường hợp và nhóm đối chứng, và hơn nữa không phát hiện thấy bất kỳ biến thể đột biến hiếm nào trong số 259 đối tượng. KẾT LUẬN Kết quả của chúng tôi không ủng hộ mối liên quan của các biến thể gen BDNF với MDD ở những người trẻ tuổi trong quần thể người Hán."} +{"idx": 1870040, "en": "BACKGROUND Rapid diagnostic test (RDT) of norovirus and rotavirus is commonly used for outbreak screening and patient management. Varying accuracy of the test and cross-reactivity has been reported and could affect the outcome of management. The primary purpose of this study is to provide the accuracy of norovirus and rotavirus rapid diagnostic tests and to analyze the cross-reactivity of both tests. MATERIALS AND METHODS Stool samples collected from every acute diarrhea patient aged <15 years old who was admitted at Bhumibol Adulyadej Hospital, Bangkok, Thailand, from November 2014 to September 2016 underwent the following test: QuickNaviTM - Norovirus2 for norovirus, VIKIA® Rota-Adeno for rotavirus, and aerobic bacterial culture. Real-time reverse transcription polymerase chain reaction was used as a gold standard for virus detection. False-positive results determined cross-reactivity. RESULTS From 358 stool specimens, the sensitivity of RDTs for norovirus and rotavirus was 27.5% and 44.8%, respectively. The specificity of RDTs for norovirus and rotavirus was 97.7% and 91.6%, respectively. False positive results of RDT for norovirus occurred in 6 samples (1.7%) and 22 samples (6.1%) in RDT for rotavirus. Rotavirus RDT was found to have cross-reactivity with 11 norovirus infection and 3 bacterial infected stools. CONCLUSION We found that the RDTs for both rotavirus and norovirus have high specificity but low sensitivity. Cross-reactivity was observed in positive rotavirus RDT with half of it being norovirus.", "gemini": "BỐI CẢNH Xét nghiệm chẩn đoán nhanh (RDT) norovirus và rotavirus thường được sử dụng để sàng lọc dịch bệnh và quản lý bệnh nhân. Độ chính xác khác nhau của xét nghiệm và phản ứng chéo đã được báo cáo và có thể ảnh hưởng đến kết quả quản lý. Mục đích chính của nghiên cứu này là cung cấp độ chính xác của các xét nghiệm chẩn đoán nhanh norovirus và rotavirus và phân tích phản ứng chéo của cả hai xét nghiệm. VẬT LIỆU VÀ PHƯƠNG PHÁP Các mẫu phân được thu thập từ mọi bệnh nhân tiêu chảy cấp có độ tuổi <15 tuổi được nhập viện tại Bệnh viện Bhumibol Adulyadej, Bangkok, Thái Lan, từ tháng 11 năm 2014 đến tháng 9 năm 2016 đã được tiến hành xét nghiệm sau: QuickNaviTM - Norovirus2 đối với norovirus, VIKIA® Rota-Adeno đối với rotavirus và nuôi cấy vi khuẩn hiếu khí. Phản ứng chuỗi polymerase phiên mã ngược thời gian thực được sử dụng làm tiêu chuẩn vàng để phát hiện vi-rút. Kết quả dương tính giả xác định phản ứng chéo. KẾT QUẢ Từ 358 mẫu phân, độ nhạy của RDT đối với norovirus và rotavirus lần lượt là 27,5% và 44,8%. Độ đặc hiệu của RDT đối với norovirus và rotavirus lần lượt là 97,7% và 91,6%. Kết quả dương tính giả của RDT đối với norovirus xảy ra ở 6 mẫu (1,7%) và 22 mẫu (6,1%) ở RDT đối với rotavirus. RDT rotavirus được phát hiện có phản ứng chéo với 11 trường hợp nhiễm norovirus và 3 trường hợp phân bị nhiễm vi khuẩn. KẾT LUẬN Chúng tôi thấy rằng RDT đối với cả rotavirus và norovirus có độ đặc hiệu cao nhưng độ nhạy thấp. Phản ứng chéo được quan sát thấy ở RDT rotavirus dương tính với một nửa trong số đó là norovirus."} +{"idx": 1870041, "en": "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can be detected via a nasopharyngeal swab and in sputum, blood, urine, and feces. However, there is only limited data on the real-time reverse transcriptase polymerase chain reaction (RT-PCR) results of coronavirus disease 2019 (COVID-19) patients with pleural fluid. We report a case of COVID-19 with SARS-CoV-2 detected in both sputum and pleural fluid. A 68-year-old male patient came to the hospital with a chief complaint of dyspnea. He was diagnosed with lung cancer. A biopsy was performed, and a pneumothorax was found. As a result, a chest tube was placed into the right pleural space. During his hospital stay, the patient was confirmed as COVID-19 positive. We identified the presence of SARS-CoV-2 through real-time RT-PCR assay from the pleural fluid. Although pleural effusion is an uncommon finding in the COVID-19, care should be taken to avoid exposure when handling the pleural fluid sample.", "gemini": "Virus corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2) có thể được phát hiện thông qua tăm bông mũi họng và trong đờm, máu, nước tiểu và phân. Tuy nhiên, chỉ có dữ liệu hạn chế về kết quả phản ứng chuỗi polymerase phiên mã ngược thời gian thực (RT-PCR) của bệnh nhân mắc bệnh do virus corona 2019 (COVID-19) có dịch màng phổi. Chúng tôi báo cáo một trường hợp COVID-19 có SARS-CoV-2 được phát hiện trong cả đờm và dịch màng phổi. Một bệnh nhân nam 68 tuổi đến bệnh viện với triệu chứng khó thở. Ông được chẩn đoán mắc bệnh ung thư phổi. Một sinh thiết đã được thực hiện và phát hiện ra tràn khí màng phổi. Kết quả là, một ống dẫn lưu ngực được đặt vào khoang màng phổi phải. Trong thời gian nằm viện, bệnh nhân được xác nhận dương tính với COVID-19. Chúng tôi xác định sự hiện diện của SARS-CoV-2 thông qua xét nghiệm RT-PCR thời gian thực từ dịch màng phổi. Mặc dù tràn dịch màng phổi là một phát hiện không phổ biến ở COVID-19, nhưng cần thận trọng để tránh tiếp xúc khi xử lý mẫu dịch màng phổi."} +{"idx": 1870042, "en": "Disseminated adenovirus infections (d-ADV) after hematopoietic cell transplant (HCT) are often fatal with limited treatment options. Brincidofovir (BCV) a lipid ester of cidofovir is developed for this indication. We report four pediatric HCT recipients with d-ADV treated successfully with BCV.", "gemini": "Nhiễm adenovirus lan tỏa (d-ADV) sau khi ghép tế bào tạo máu (HCT) thường gây tử vong với các lựa chọn điều trị hạn chế. Brincidofovir (BCV) là este lipid của cidofovir được phát triển để điều trị tình trạng này. Chúng tôi báo cáo bốn bệnh nhi được ghép tế bào gốc tạo máu bị d-ADV được điều trị thành công bằng BCV."} +{"idx": 1870043, "en": "Cushing's syndrome is characterized by excessive cortisol and immuno-suppression. We experienced a case of Cushing's syndrome caused by adrenocortical carcinoma that was complicated by multiple opportunistic infections. A 37-year-old woman with adrenocortical carcinoma (ACC) presented with decreased mental ability and high fever one week after undergoing chemotherapy. Her initial blood culture revealed methicillin-resistant Staphylococcus aureus bacteremia accompanied by septic pneumonia. We admitted her to the intensive care unit and treated her for invasive pulmonary aspergillosis (IPA), Pneumocystis jirovecii pneumonia (PJP), candidemia, and Stenotrophomonas maltophilia pneumonia with broad-spectrum antibiotics and antifungal agents. Nevertheless, her clinical course worsened and she died. Herein, we report a case of Cushing's syndrome associated with cortisol-secreting ACC that presented with multiple opportunistic infections, including MRSA bacteremia, septic pneumonia, candidemia, PJP, and IPA, illuminating a relationship between hypercortisolemia and opportunistic infections.", "gemini": "Hội chứng Cushing được đặc trưng bởi tình trạng cortisol dư thừa và ức chế miễn dịch. Chúng tôi đã gặp một trường hợp hội chứng Cushing do ung thư vỏ thượng thận có biến chứng là nhiều loại nhiễm trùng cơ hội. Một phụ nữ 37 tuổi bị ung thư vỏ thượng thận (ACC) xuất hiện tình trạng giảm khả năng nhận thức và sốt cao một tuần sau khi trải qua hóa trị. Kết quả nuôi cấy máu ban đầu của cô cho thấy tình trạng nhiễm khuẩn huyết tụ cầu vàng kháng methicillin kèm theo viêm phổi nhiễm trùng. Chúng tôi đã đưa cô vào phòng chăm sóc đặc biệt và điều trị chứng nấm phổi xâm lấn (IPA), viêm phổi do Pneumocystis jirovecii (PJP), nhiễm nấm candida và viêm phổi do Stenotrophomonas maltophilia bằng thuốc kháng sinh phổ rộng và thuốc chống nấm. Tuy nhiên, tình trạng lâm sàng của cô ngày càng xấu đi và cô đã tử vong. Trong báo cáo này, chúng tôi trình bày một trường hợp hội chứng Cushing liên quan đến ACC tiết cortisol có biểu hiện là nhiều loại nhiễm trùng cơ hội, bao gồm nhiễm khuẩn huyết MRSA, viêm phổi nhiễm trùng, nhiễm nấm candida, PJP và IPA, làm sáng tỏ mối quan hệ giữa tình trạng tăng cortisol huyết và nhiễm trùng cơ hội."} +{"idx": 1870044, "en": "Cytomegalovirus (CMV) causes severe infection in immunocompromised patients, especially those with acquired immunodeficiency syndrome (AIDS), those who have undergone organ transplantation, and/or those who are being treated with steroids. In patients with AIDS, CMV is the most common pathogen that causes opportunistic infections. Here, we present a case of liver abscess due to CMV in a 58-year-old man diagnosed with AIDS. At the time of the study, his CD4 T lymphocyte count was 39 cells/mm³, and his human immunodeficiency virus (HIV) ribonucleic acid (RNA) level was 411,000 copies/mL. Upon presentation, he complained of upper abdominal pain. Ultrasonography-guided percutaneous drainage of the liver abscess was performed, and polymerase chain reaction PCR analysis of the drained pus was positive for CMV. He was treated with intravenous ganciclovir, after which he recovered fully. In conclusion, our patient represents, to our knowledge, the first described case of liver abscess caused by CMV in an AIDS patient, which was probably facilitated by severe immunodeficiency.", "gemini": "Cytomegalovirus (CMV) gây ra tình trạng nhiễm trùng nghiêm trọng ở những bệnh nhân suy giảm miễn dịch, đặc biệt là những người mắc hội chứng suy giảm miễn dịch mắc phải (AIDS), những người đã trải qua ghép tạng và/hoặc những người đang được điều trị bằng steroid. Ở những bệnh nhân mắc AIDS, CMV là tác nhân gây bệnh phổ biến nhất gây ra các bệnh nhiễm trùng cơ hội. Ở đây, chúng tôi trình bày một trường hợp áp xe gan do CMV ở một người đàn ông 58 tuổi được chẩn đoán mắc AIDS. Vào thời điểm nghiên cứu, số lượng tế bào lympho T CD4 của ông là 39 tế bào/mm³ và mức axit ribonucleic (RNA) của vi-rút gây suy giảm miễn dịch ở người (HIV) là 411.000 bản sao/mL. Khi nhập viện, ông than phiền về tình trạng đau bụng trên. Đã tiến hành dẫn lưu ổ áp xe gan bằng siêu âm và phân tích phản ứng chuỗi polymerase (PCR) đối với mủ dẫn lưu cho kết quả dương tính với CMV. Ông được điều trị bằng ganciclovir tiêm tĩnh mạch, sau đó ông đã hồi phục hoàn toàn. Tóm lại, theo hiểu biết của chúng tôi, bệnh nhân của chúng tôi là trường hợp đầu tiên được mô tả về áp xe gan do CMV ở một bệnh nhân AIDS, có thể là do tình trạng suy giảm miễn dịch nghiêm trọng."} +{"idx": 1870045, "en": "Scrub typhus present with a variety clinical manifestations caused by Orientia tsutsugamushi, it is sometimes accompanied by severe complications. The clinical course of this complication is not well clear. Herein, we first report a case with acute cholangitis and acalculous cholecystitis caused by the Boryong genotype of O. tsutsugamushi. A 82-year-old woman presented with fever and acute abdominal pain on the right upper quadrant. Initially, the patient showed an abnormality of liver enzymes of cholestatic pattern. An acute cholangitis and cholecystitis were suspected on the enhanced computed tomography (CT), and emergent endoscopic retrograde cholangiopancreatography (ERCP) performed. However, neither ERCP nor CT detected common bile duct stones or sludge. The patient's illness progressed despite bile drainage and antibiotic therapy. On the fifth day in hospital, an immunofluorescence IgG assay for O. tsutsugamushi was positive, and the patient newly developed a skin rash and an eschar lesion on the right abdomen. The polymerase chain reaction amplification of Orientia genes from blood and bile was positive, genotype of both samples was identified the Boryong type. The presentation of scrub typhus as an acute cholangitis is not reported till now. In endemic areas, scrub typhus would be considered a rare etiology of acute cholangitis.", "gemini": "Sốt phát ban do vi khuẩn Orientia tsutsugamushi gây ra có nhiều biểu hiện lâm sàng khác nhau, đôi khi đi kèm với các biến chứng nghiêm trọng. Diễn biến lâm sàng của biến chứng này chưa được làm rõ. Trong bài báo này, chúng tôi báo cáo một trường hợp viêm đường mật cấp và viêm túi mật không sỏi do chủng Boryong của O. tsutsugamushi. Một phụ nữ 82 tuổi đến khám với biểu hiện sốt và đau bụng cấp ở phần bụng trên bên phải. Ban đầu, bệnh nhân có xét nghiệm men gan bất thường theo kiểu ứ mật. Chụp cắt lớp vi tính (CT) tăng cường nghi ngờ viêm đường mật cấp và viêm túi mật, và nội soi ngược dòng mật tụy (ERCP) cấp cứu đã được thực hiện. Tuy nhiên, cả ERCP và CT đều không phát hiện thấy sỏi hoặc bùn trong ống mật chủ. Bệnh của bệnh nhân tiến triển mặc dù đã dẫn lưu mật và điều trị bằng kháng sinh. Vào ngày thứ năm nhập viện, xét nghiệm miễn dịch huỳnh quang IgG đối với O. tsutsugamushi có kết quả dương tính và bệnh nhân mới phát ban và tổn thương hoại tử ở bụng bên phải. Khuếch đại phản ứng chuỗi polymerase gen Orientia từ máu và mật có kết quả dương tính, kiểu gen của cả hai mẫu đều được xác định là loại Boryong. Biểu hiện của sốt phát ban như viêm đường mật cấp cho đến nay vẫn chưa được báo cáo. Ở những vùng lưu hành, sốt phát ban có thể được coi là một nguyên nhân hiếm gặp của viêm đường mật cấp."} +{"idx": 1870046, "en": "Vibrio cholerae is a pathogen known to cause the waterborne epidemic disease cholera. Overall, V. cholerae O1 or O139 strains produce the cholera toxin that cause gastroenteritis, resulting in watery diarrhea. Most of the enterocolitis caused by V. cholerae can be easily treated with fluid therapy and conservative care. However, V. cholerae non-O1/O139 strains can cause extraintestinal infections, such as wound infection or sepsis, in immunocompromised patients. The clinical course of these infections is very similar to that of V. vulnificus infection. We report about a 52-year-old man without previous underlying disease who was diagnosed with V. cholerae non-O1/O139 infection and died within 72 hours after admission to the intensive care unit.", "gemini": "Vibrio cholerae là một tác nhân gây bệnh được biết đến gây ra bệnh tả dịch do nước. Nhìn chung, các chủng V. cholerae O1 hoặc O139 sản xuất độc tố tả gây ra viêm dạ dày ruột, dẫn đến tiêu chảy phân nước. Hầu hết các trường hợp viêm ruột do V. cholerae gây ra đều có thể dễ dàng điều trị bằng liệu pháp truyền dịch và chăm sóc bảo tồn. Tuy nhiên, các chủng V. cholerae không phải O1/O139 có thể gây ra các nhiễm trùng ngoài đường ruột, chẳng hạn như nhiễm trùng vết thương hoặc nhiễm trùng huyết, ở những bệnh nhân suy giảm miễn dịch. Diễn biến lâm sàng của các bệnh nhiễm trùng này rất giống với nhiễm trùng V. vulnificus. Chúng tôi báo cáo về một người đàn ông 52 tuổi không có bệnh nền trước đó được chẩn đoán nhiễm V. cholerae không phải O1/O139 và tử vong trong vòng 72 giờ sau khi nhập khoa hồi sức tích cực."} +{"idx": 1870047, "en": "BACKGROUND Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection is not differentiated clinically from other respiratory infections, and intensive care units (ICUs) are vulnerable to in-hospital transmission due to interventions inducing respiratory aerosols. This study evaluated the effectiveness of universal SARS-CoV-2 screening in ICUs in terms of screened-out cases and reduction in anxiety of healthcare personnel (HCP). MATERIALS AND METHODS This prospective single-armed observational study was conducted in 2 ICUs of a single hospital. The number of patients diagnosed with SARS-CoV-2 infection by the screening program and healthcare workers in ICUs that visited the SARS-CoV-2 screening clinic or infection clinic were investigated. RESULTS During the 7-week study period, no positive screening case was reported among a total of 142 patients. Among 86 HCP in the ICUs, only 2 HCP sought medical consultation for SARS-CoV-2 infection during the initial 2 weeks. CONCLUSION A universal screening program for SARS-CoV-2 infection in ICUs with the coordination of other countermeasures in the hospital was reasonably effective in preventing in-hospital transmission in a pandemic situation and making clinical practices and HCP stable.", "gemini": "BỐI CẢNH Nhiễm trùng hội chứng hô hấp cấp tính nặng do virus corona 2 (SARS-CoV-2) không được phân biệt lâm sàng với các bệnh nhiễm trùng đường hô hấp khác và các đơn vị chăm sóc tích cực (ICU) dễ bị lây truyền trong bệnh viện do các can thiệp gây ra các hạt khí dung đường hô hấp. Nghiên cứu này đánh giá hiệu quả của việc sàng lọc SARS-CoV-2 toàn diện ở ICU về các trường hợp được sàng lọc và giảm lo lắng cho nhân viên y tế (HCP). VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu quan sát có triển vọng một nhánh này được tiến hành tại 2 ICU của một bệnh viện duy nhất. Số lượng bệnh nhân được chẩn đoán nhiễm SARS-CoV-2 bằng chương trình sàng lọc và nhân viên y tế tại ICU đến khám tại phòng sàng lọc SARS-CoV-2 hoặc phòng khám nhiễm trùng đã được điều tra. KẾT QUẢ Trong thời gian nghiên cứu kéo dài 7 tuần, không có trường hợp sàng lọc dương tính nào được báo cáo trong tổng số 142 bệnh nhân. Trong số 86 nhân viên y tế tại ICU, chỉ có 2 nhân viên y tế tìm kiếm tư vấn y tế về nhiễm SARS-CoV-2 trong 2 tuần đầu. KẾT LUẬN Một chương trình sàng lọc toàn diện đối với nhiễm SARS-CoV-2 ở ICU với sự phối hợp của các biện pháp đối phó khác trong bệnh viện có hiệu quả hợp lý trong việc ngăn ngừa lây truyền trong bệnh viện trong tình huống đại dịch và giúp các hoạt động lâm sàng và nhân viên y tế ổn định."} +{"idx": 1870048, "en": "Korea had been one of hyperendemic countries of human parasitic infections until 1970s. In 1966, the Law for the Prevention of Parasitic Diseases was enacted, and the nationwide anti-parasitic control program began in 1969. The initiation of the national program was supported financially by Japan. The program included screening of whole students in Korea and treatment of all egg positive cases twice a year, and ended in 1995. In addition to student program, deworming campaign was run in the community, and 8 national status surveys were implemented from 1971 to 2012. Whole helminth egg positive rate was 84.3% in 1971 and decreased to 2.6% in 2012. Ascaris and other intestinal nematodes, Paragonimus, Taenia, and intestinal protozoa had decreased significantly throughout the country, but Clonorchis sinensis and intestinal trematodes are still prevalent locally in endemic areas. Lymphatic filariasis had been endemic in Jeju-do and other southern islands but elimination was endorsed in 2008. The control of parasitic infection in Korea was successful with statistical prevalence data, which can be a benchmarking model. In conclusion, the successful control in Korea could be achieved by social agreement of the priority, professional guidelines and systematic approach with good anthelminthics supply, and simultaneous economic growth.", "gemini": "Cho đến những năm 1970, Hàn Quốc là một trong những quốc gia có tỷ lệ lưu hành cao các bệnh nhiễm trùng ký sinh trùng ở người. Vào năm 1966, Luật Phòng chống Bệnh ký sinh trùng đã được ban hành và chương trình kiểm soát ký sinh trùng toàn quốc bắt đầu vào năm 1969. Nhật Bản đã hỗ trợ tài chính cho việc khởi xướng chương trình quốc gia này. Chương trình bao gồm việc sàng lọc toàn bộ học sinh tại Hàn Quốc và điều trị cho tất cả các trường hợp có trứng ký sinh trùng hai lần một năm, và kết thúc vào năm 1995. Ngoài chương trình dành cho học sinh, chiến dịch tẩy giun đã được tiến hành trong cộng đồng và 8 cuộc điều tra tình trạng sức khỏe toàn quốc đã được thực hiện từ năm 1971 đến năm 2012. Tỷ lệ dương tính với trứng giun toàn bộ là 84,3% vào năm 1971 và giảm xuống còn 2,6% vào năm 2012. Giun đũa và các loại giun tròn đường ruột khác, sán lá phổi, sán dây và động vật nguyên sinh đường ruột đã giảm đáng kể trên toàn quốc, nhưng sán lá gan lớn và sán lá ruột vẫn phổ biến cục bộ ở các vùng lưu hành. Bệnh giun chỉ bạch huyết đã từng là bệnh lưu hành ở đảo Jeju và các đảo phía nam khác nhưng đã được loại trừ vào năm 2008. Việc kiểm soát nhiễm ký sinh trùng ở Hàn Quốc đã thành công với dữ liệu tỷ lệ lưu hành thống kê, có thể là mô hình chuẩn mực. Tóm lại, việc kiểm soát thành công ở Hàn Quốc có thể đạt được nhờ sự đồng thuận xã hội về ưu tiên, các hướng dẫn chuyên môn và cách tiếp cận có hệ thống với nguồn cung thuốc diệt giun tốt và tăng trưởng kinh tế đồng thời."} +{"idx": 1870049, "en": "Vivax malaria which had been highly prevalent in Korea disappeared rapidly from the 1960s to 1984 when domestic occurrence of cases stopped. However, malaria reemerged in 1993 near the demilitarized zone (DMZ) bordering with North Korea. The number of patients thereafter increased exponentially year after year totaling 35,526 cases by the end of 2015. A small number of cases (1 - 53 patients annually) also occurred among the United States military personnel camping in Korea. However, after the 2010s the number of annual malaria cases has been decreasing slowly in Korea. Several reports on malaria situation in North Korea described high malaria prevalence after 1997 which peaked during 1999 - 2002 and has been decreasing thereafter. At the beginning of the reemergence, the majority of cases (60 - 90%) were soldiers aged 20 - 25 years camping around the northern parts of Gyeonggi-do and Gangwon-do (Province), Korea just facing the DMZ. However, as the outbreak continued more civilians were infected. The course of illness was relatively mild, and chemotherapy with chloroquine in combination with primaquine was successful in most of the patients. Mass chemoprophylaxis combined with mosquito control activities greatly contributed to the decline of malaria situation among Korean military soldiers.", "gemini": "Bệnh sốt rét vivax từng lưu hành rất cao ở Hàn Quốc đã biến mất nhanh chóng từ những năm 1960 đến 1984 khi các trường hợp mắc bệnh trong nước không còn nữa. Tuy nhiên, bệnh sốt rét đã tái phát vào năm 1993 gần khu phi quân sự (DMZ) giáp với Bắc Triều Tiên. Sau đó, số lượng bệnh nhân tăng theo cấp số nhân theo từng năm, tổng cộng có 35.526 trường hợp vào cuối năm 2015. Một số ít trường hợp (1 - 53 bệnh nhân hàng năm) cũng xảy ra trong số quân nhân Hoa Kỳ đóng quân tại Hàn Quốc. Tuy nhiên, sau những năm 2010, số ca mắc bệnh sốt rét hàng năm đã giảm dần ở Hàn Quốc. Một số báo cáo về tình hình sốt rét ở Bắc Triều Tiên mô tả tỷ lệ lưu hành sốt rét cao sau năm 1997, đạt đỉnh trong giai đoạn 1999 - 2002 và sau đó đã giảm. Vào thời điểm tái phát, phần lớn các trường hợp (60 - 90%) là những người lính từ 20 - 25 tuổi đóng quân quanh các vùng phía bắc của Gyeonggi-do và Gangwon-do (Tỉnh), Hàn Quốc ngay đối diện với DMZ. Tuy nhiên, khi dịch bệnh tiếp tục, nhiều dân thường hơn đã bị nhiễm bệnh. Quá trình bệnh diễn biến tương đối nhẹ và hóa trị bằng chloroquine kết hợp với primaquine đã thành công ở hầu hết bệnh nhân. Phòng ngừa bằng hóa chất hàng loạt kết hợp với các hoạt động kiểm soát muỗi đã đóng góp rất lớn vào việc giảm tình trạng sốt rét ở những người lính quân đội Hàn Quốc."} +{"idx": 1870050, "en": "Coronaviruses have caused serious Severe Acute Respiratory Syndrome (SARS), Middle East Respiratory Syndrome (MERS), and Coronavirus Disease 2019 (COVID-19) outbreaks, and only remdesivir has been recently indicated for the treatment of COVID-19. In the line of therapeutic options for SARS and MERS, this study aims to summarize the current clinical evidence of treatment options for COVID-19. In general, the combination of antibiotics, ribavirin, and corticosteroids was considered as a standard treatment for patients with SARS. The addition of this conventional treatment with lopinavir/ritonavir, interferon, and convalescent plasma showed potential clinical improvement. For patients with MERS, ribavirin, lopinavir/ritonavir, interferon, and convalescent plasma were continuously recommended. However, a high-dose of corticosteroid was suggested for severe cases only. The use of lopinavir/ritonavir and convalescent plasma was commonly reported. There was limited evidence for the effect of corticosteroids, other antiviral drugs like ribavirin, and favipiravir. Monoclonal antibody of tocilizumab and antimalarial agents of chloroquine and hydroxychloroquine were also introduced. Among antibiotics for infection therapy, azithromycin was suggested. In conclusion, this study showed the up-to-date evidence of treatment options for COVID-19 that is helpful for the therapy selection and the development of further guidelines and recommendations. Updates of on-going clinical trials and observational studies may confirm the current findings.", "gemini": "Virus corona đã gây ra các đợt bùng phát Hội chứng hô hấp cấp tính nặng (SARS), Hội chứng hô hấp Trung Đông (MERS) và Bệnh do virus corona 2019 (COVID-19) nghiêm trọng và chỉ có remdesivir mới được chỉ định gần đây để điều trị COVID-19. Trong số các lựa chọn điều trị cho SARS và MERS, nghiên cứu này nhằm mục đích tóm tắt các bằng chứng lâm sàng hiện tại về các lựa chọn điều trị cho COVID-19. Nhìn chung, sự kết hợp của thuốc kháng sinh, ribavirin và corticosteroid được coi là phương pháp điều trị tiêu chuẩn cho bệnh nhân SARS. Việc bổ sung phương pháp điều trị thông thường này bằng lopinavir/ritonavir, interferon và huyết tương hồi phục đã cho thấy khả năng cải thiện lâm sàng. Đối với bệnh nhân MERS, ribavirin, lopinavir/ritonavir, interferon và huyết tương hồi phục liên tục được khuyến cáo. Tuy nhiên, chỉ nên dùng corticosteroid liều cao cho các trường hợp nặng. Việc sử dụng lopinavir/ritonavir và huyết tương hồi phục thường được báo cáo. Có bằng chứng hạn chế về tác dụng của corticosteroid, các thuốc kháng vi-rút khác như ribavirin và favipiravir. Kháng thể đơn dòng tocilizumab và thuốc chống sốt rét chloroquine và hydroxychloroquine cũng đã được đưa vào sử dụng. Trong số các loại kháng sinh dùng để điều trị nhiễm trùng, azithromycin đã được đề xuất. Tóm lại, nghiên cứu này cho thấy bằng chứng mới nhất về các lựa chọn điều trị cho COVID-19, rất hữu ích cho việc lựa chọn phương pháp điều trị và phát triển các hướng dẫn và khuyến cáo thêm. Việc cập nhật các thử nghiệm lâm sàng đang diễn ra và các nghiên cứu quan sát có thể xác nhận các phát hiện hiện tại."} +{"idx": 1870051, "en": "Coronavirus disease 2019 (COVID-19) has spread widely across the world since January 2020. There are many challenges when caring for patients with COVID-19, one of which is infection prevention and control. In particular, in cases where surgery must absolutely be performed, special infection control may be required in order to perform surgery without spreading infection within the hospital. We aim to present potentially useful recommendations for non-deferrable surgery for COVID-19 patients based on in vivo and in vitro research and clinical experiences from many countries.", "gemini": "Bệnh do virus corona 2019 (COVID-19) đã lan rộng khắp thế giới kể từ tháng 1 năm 2020. Có nhiều thách thức khi chăm sóc bệnh nhân mắc COVID-19, một trong số đó là phòng ngừa và kiểm soát nhiễm trùng. Đặc biệt, trong những trường hợp bắt buộc phải phẫu thuật, có thể cần kiểm soát nhiễm trùng đặc biệt để thực hiện phẫu thuật mà không lây lan nhiễm trùng trong bệnh viện. Chúng tôi muốn trình bày những khuyến nghị có thể hữu ích cho các ca phẫu thuật không thể trì hoãn đối với bệnh nhân COVID-19 dựa trên các nghiên cứu in vivo và in vitro cũng như kinh nghiệm lâm sàng từ nhiều quốc gia."} +{"idx": 1870052, "en": "Acinetobacter baumannii is one of the most important etiologies of nosocomial infections in recent years mainly because of increasing in frequency of multidrug and pan-resistant pathogens. Meningitis caused by this organism is a dilemma; because polymyxins are the only effective antibiotics against pan-resistant serotypes, but have poor penetration via blood brain barrier; however, it has still remained uncertain whether the intravenous therapy with these agents is an effective treatment with the sufficient concentration of the drug in the cerebrospinal fluid. Herein, we report a neonate who suffered from pan-resistant A. baumannii nosocomial meningitis successfully treated with intravenous colistin combined with meropenem and rifampin. It seems that intravenous colistin at least in combination with rifampin and meropenem might be considered as an option to try in patients in whom daily intrathecal injection or insertion of intraventricular device is not possible.", "gemini": "Acinetobacter baumannii là một trong những nguyên nhân quan trọng nhất gây nhiễm trùng bệnh viện trong những năm gần đây chủ yếu là do tần suất gia tăng của các tác nhân gây bệnh đa kháng và kháng toàn phần. Viêm màng não do vi khuẩn này là một tình trạng khó xử; bởi vì polymyxin là loại kháng sinh duy nhất có hiệu quả chống lại các huyết thanh kháng toàn phần, nhưng lại thẩm thấu kém qua hàng rào máu não; tuy nhiên, vẫn chưa chắc chắn liệu liệu pháp tiêm tĩnh mạch bằng các tác nhân này có phải là phương pháp điều trị hiệu quả với nồng độ thuốc đủ trong dịch não tủy hay không. Trong bài báo này, chúng tôi báo cáo về một trẻ sơ sinh bị viêm màng não do A. baumannii kháng toàn phần ở bệnh viện đã được điều trị thành công bằng cách kết hợp colistin tiêm tĩnh mạch với meropenem và rifampin. Có vẻ như colistin tiêm tĩnh mạch ít nhất khi kết hợp với rifampin và meropenem có thể được coi là một lựa chọn để thử ở những bệnh nhân không thể tiêm thuốc vào khoang dưới màng nhện hàng ngày hoặc không thể lắp đặt thiết bị nội thất thất."} +{"idx": 1870053, "en": "The use of ureteral access sheaths (UAS) is common practice during routine flexible ureteroscopy procedures. However, debates and concerns continue amongst endourologists on routine UAS placement. UAS placement allows for multiple passages of the ureteroscope, decreases intrarenal pressure, and may improve stone-free rates. However, concerns for the UAS's effectiveness in these claimed benefits and complications related to UAS placement has been documented and investigated by many. In this review, we will discuss the controversies surrounding the placement of UAS during ureteroscopy.", "gemini": "Việc sử dụng bao thông niệu quản (UAS) là thực hành phổ biến trong các thủ thuật nội soi niệu quản mềm thông thường. Tuy nhiên, các cuộc tranh luận và lo ngại vẫn tiếp tục giữa các bác sĩ nội soi niệu quản về việc đặt UAS thường quy. Đặt UAS cho phép nội soi niệu quản đi qua nhiều lần, giảm áp lực trong thận và có thể cải thiện tỷ lệ sạch sỏi. Tuy nhiên, những lo ngại về hiệu quả của UAS trong những lợi ích được tuyên bố này và các biến chứng liên quan đến việc đặt UAS đã được nhiều người ghi nhận và nghiên cứu. Trong bài đánh giá này, chúng tôi sẽ thảo luận về những tranh cãi xung quanh việc đặt UAS trong quá trình nội soi niệu quản."} +{"idx": 1870054, "en": "PURPOSE This study aimed to determine the effectiveness and safety of partial nephrectomy (PN) without ischemia compared with PN with warm ischemia for reducing the deterioration in renal function in patients with cT1 renal tumors. MATERIALS AND METHODS We conducted a systematic review that included patients over 18 years of age who underwent PN with or without warm ischemia for cT1 renal tumors. The primary outcome was impaired renal function. A search strategy was performed in MEDLINE, EMBASE, LILACS, CENTRAL, the article reference lists, and the unpublished literature to reach saturation of the information. We assessed the risk of bias with the methodological index for nonrandomized studies (MINORS) tool, and we performed a meta-analysis according to the type of variable. RESULTS We found a total of 5,682 articles, of which 14 met the inclusion criteria. Seven studies evaluated renal function, identifying a difference in means (MD) of 3.50 (95% confidence interval [CI], 1.16 to 5.83), favoring no ischemia. We did not find any significant differences regarding intraoperative bleeding or operative time (MD, 55 mL; 95% CI, -33.16 to 144.08; and MD, 1.87; 95% CI, -20.47 to 24.21; respectively). CONCLUSIONS In this study, PN without ischemia showed a decrease in deterioration of the estimated glomerular filtration rate compared with warm ischemia.", "gemini": "MỤC ĐÍCH Nghiên cứu này nhằm xác định hiệu quả và tính an toàn của cắt bỏ thận một phần (PN) không thiếu máu cục bộ so với PN có thiếu máu cục bộ ấm trong việc làm giảm sự suy giảm chức năng thận ở những bệnh nhân có khối u thận cT1. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi đã tiến hành một đánh giá có hệ thống bao gồm những bệnh nhân trên 18 tuổi đã trải qua phẫu thuật PN có hoặc không có thiếu máu cục bộ ấm đối với khối u thận cT1. Kết quả chính là suy giảm chức năng thận. Một chiến lược tìm kiếm đã được thực hiện trong MEDLINE, EMBASE, LILACS, CENTRAL, danh sách tham chiếu bài viết và các tài liệu chưa công bố để đạt được sự bão hòa thông tin. Chúng tôi đã đánh giá nguy cơ thiên vị bằng công cụ chỉ số phương pháp luận cho các nghiên cứu không ngẫu nhiên (MINORS) và chúng tôi đã thực hiện phân tích tổng hợp theo loại biến. KẾT QUẢ Chúng tôi đã tìm thấy tổng cộng 5.682 bài báo, trong đó có 14 bài đáp ứng tiêu chuẩn đưa vào. Bảy nghiên cứu đã đánh giá chức năng thận, xác định sự khác biệt về giá trị trung bình (MD) là 3,50 (khoảng tin cậy 95% [CI], 1,16 đến 5,83), ủng hộ không thiếu máu cục bộ. Chúng tôi không tìm thấy bất kỳ sự khác biệt đáng kể nào về chảy máu trong khi phẫu thuật hoặc thời gian phẫu thuật (MD, 55 mL; 95% CI, -33,16 đến 144,08; và MD, 1,87; 95% CI, -20,47 đến 24,21; tương ứng). KẾT LUẬN Trong nghiên cứu này, PN không thiếu máu cục bộ cho thấy sự suy giảm tốc độ lọc cầu thận ước tính giảm so với thiếu máu cục bộ ấm."} +{"idx": 1870055, "en": "PURPOSE Poor awakening in patients with enuresis has been assumed to be an adaptation to the chronic influence of arousal stimuli like lower urinary tract dysfunction (LUTD). This study aimed to examine the effect of controlling LUTD on improvement of awakening and cure of enuresis. MATERIALS AND METHODS Data for 119 enuretic patients with overactive bladder were retrospectively analyzed. The patients received urotherapy, laxatives, and anticholinergic agents. LUTD symptoms and enuresis were regularly monitored every 3 months. History of waking up because of bedwetting (ability to awaken, AA) was used as a surrogate marker of arousal and was graded at baseline and every 3 months of treatment. Changing distribution of each grade of AA was associated with other LUTD symptoms. Multivariate analysis was applied to understand whether the lack of improvement in AA might harbor any prognostic implications regarding cure of enuresis. RESULTS Decreasing number of LUTD with treatment corresponded to increasing number of better AA. Enuresis was resolved completely in 88 patients in a median time of 7 months. Failure to show even single episode of awakening before bedwetting within 6 months of treatment and persistent daytime incontinence were identified as risk factors for treatment resistance until 18 months of treatment. CONCLUSIONS Controlling LUTD symptoms in the management of nonmonosymptomatic enuresis was crucial for not only the treatment of enuresis but also for allowing the patients to obtain better arousal. AA tended to improve subsequent to bladder control and may contribute to the cure of enuresis.", "gemini": "MỤC ĐÍCH Người ta cho rằng việc đánh thức kém ở những bệnh nhân bị đái dầm là một sự thích nghi với ảnh hưởng mãn tính của các kích thích gây tỉnh táo như rối loạn chức năng đường tiết niệu dưới (LUTD). Nghiên cứu này nhằm mục đích kiểm tra tác dụng của việc kiểm soát LUTD đối với việc cải thiện sự tỉnh táo và chữa khỏi chứng đái dầm. VẬT LIỆU VÀ PHƯƠNG PHÁP Dữ liệu của 119 bệnh nhân đái dầm có bàng quang hoạt động quá mức đã được phân tích hồi cứu. Những bệnh nhân này đã được điều trị bằng phương pháp niệu học, thuốc nhuận tràng và thuốc kháng cholinergic. Các triệu chứng LUTD và chứng đái dầm được theo dõi thường xuyên sau mỗi 3 tháng. Tiền sử thức giấc vì đái dầm (khả năng tỉnh táo, AA) được sử dụng như một dấu hiệu thay thế cho sự tỉnh táo và được phân loại theo mức độ khi mới bắt đầu và sau mỗi 3 tháng điều trị. Sự thay đổi phân bố của từng mức độ AA có liên quan đến các triệu chứng LUTD khác. Phân tích đa biến được áp dụng để hiểu liệu việc không cải thiện AA có thể ẩn chứa bất kỳ ý nghĩa tiên lượng nào liên quan đến việc chữa khỏi chứng đái dầm hay không. KẾT QUẢ Số lượng LUTD giảm khi điều trị tương ứng với số lượng AA tốt hơn tăng lên. Chứng đái dầm đã được giải quyết hoàn toàn ở 88 bệnh nhân trong thời gian trung bình là 7 tháng. Việc không biểu hiện ngay cả một cơn tỉnh táo trước khi đái dầm trong vòng 6 tháng điều trị và tình trạng són tiểu ban ngày dai dẳng được xác định là các yếu tố nguy cơ dẫn đến tình trạng kháng trị cho đến 18 tháng điều trị. KẾT LUẬN Việc kiểm soát các triệu chứng LUTD trong việc quản lý chứng đái dầm không đơn triệu chứng rất quan trọng không chỉ đối với việc điều trị chứng đái dầm mà còn giúp bệnh nhân đạt được sự tỉnh táo tốt hơn. AA có xu hướng cải thiện sau khi kiểm soát bàng quang và có thể góp phần chữa khỏi chứng đái dầm."} +{"idx": 1870056, "en": "PURPOSE To confirm feasibility of recording pressure along the length of the urethra using a multi-sensor fibre-optic pressure catheter; to identify the spatial and temporal features of changes in pressure along the urethra at sites related to specific striated pelvic floor muscles; and to investigate the relationship between urethral pressures and activation of individual pelvic floor muscles estimated from ultrasound imaging. MATERIALS AND METHODS Proof-of-concept study including one male (47 years old) and one female (33 years old). A multi-sensor fibre optic pressure catheter (10 mm sensor separation) was inserted into the urethra. Pressure data were recorded simultaneously with trans-perineal ultrasound imaging measures of pelvic floor muscle activity during sub-maximal and maximal voluntary contractions and evoked coughs. RESULTS Pressure changes along the urethra were recorded in all tasks in both participants. Face validity of interpretation of pressure measures with respect to individual muscles was supported by correlation with ultrasound-measured displacements induced by the relevant muscles. Onset of pressure increase occurred in a distal to proximal sequence in the urethra of the male but not the female during voluntary contraction. Peak urethral pressures varied in location, timing and amplitude between tasks. Evoked cough induced in the greatest urethral pressure increase across all tasks for both participants. CONCLUSIONS The high spatial resolution pressure catheter provide viable and valid recordings of urethral pressure in a male and female. Data provide preliminary evidence of sex differences in spatial and temporal distribution of urethral pressure changes.", "gemini": "MỤC ĐÍCH Xác nhận tính khả thi của việc ghi lại áp suất dọc theo chiều dài niệu đạo bằng ống thông áp suất sợi quang đa cảm biến; xác định các đặc điểm về không gian và thời gian của những thay đổi về áp suất dọc theo niệu đạo tại các vị trí liên quan đến các cơ sàn chậu có vân cụ thể; và để điều tra mối quan hệ giữa áp suất niệu đạo và sự hoạt động của từng cơ sàn chậu ước tính từ hình ảnh siêu âm. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu chứng minh khái niệm bao gồm một nam giới (47 tuổi) và một nữ giới (33 tuổi). Một ống thông áp suất sợi quang đa cảm biến (khoảng cách cảm biến 10 mm) được đưa vào niệu đạo. Dữ liệu áp suất được ghi đồng thời với các biện pháp hình ảnh siêu âm qua tầng sinh môn về hoạt động của cơ sàn chậu trong quá trình co thắt tự ý dưới mức tối đa và tối đa và ho có chủ ý. KẾT QUẢ Những thay đổi về áp suất dọc theo niệu đạo đã được ghi lại trong tất cả các nhiệm vụ ở cả hai người tham gia. Tính hợp lệ về mặt hình thức của việc giải thích các biện pháp áp suất đối với từng cơ được hỗ trợ bởi mối tương quan với các dịch chuyển được đo bằng siêu âm do các cơ liên quan gây ra. Sự khởi phát của sự gia tăng áp suất xảy ra theo trình tự từ xa đến gần ở niệu đạo của nam giới nhưng không phải ở nữ giới trong quá trình co thắt tự ý. Áp suất niệu đạo cực đại thay đổi về vị trí, thời gian và biên độ giữa các nhiệm vụ. Ho có chủ ý gây ra sự gia tăng áp suất niệu đạo lớn nhất trong tất cả các nhiệm vụ đối với cả hai người tham gia. KẾT LUẬN Ống thông áp suất có độ phân giải không gian cao cung cấp các bản ghi áp suất niệu đạo khả thi và hợp lệ ở nam và nữ. Dữ liệu cung cấp bằng chứng sơ bộ về sự khác biệt giới tính về phân bố không gian và thời gian của những thay đổi về áp suất niệu đạo."} +{"idx": 1870057, "en": "PURPOSE The Acute Cystitis Symptom Score (ACSS) used in a clinical trial comparing the phytodrug Canephron®N (BNO 1045) with an antibacterial agent (fosfomycin trometamol [FT]) in the treatment of acute uncomplicated cystitis (AC) in women was evaluated as a patient-reported outcome measure in a post hoc analysis. MATERIALS AND METHODS This double-blind, randomized, multicenter, phase III noninferiority trial was performed in 51 centers in Europe. The ACSS questionnaire was used to assess severity and course of symptoms. RESULTS The post hoc analysis included 325 patients treated with BNO 1045 and 332 patients treated with FT (total of 657 patients). The mean sum-scores of the ACSS-typical domain were comparable between groups on day 1 (BNO 1045: 10.2; FT: 10.1), and then decreased on day 4 (BNO 1045: 5.1; FT: 4.5), at end of treatment on day 8 (BNO 1045: 2.1; FT: 2.1), and at late follow-up on day 38 (BNO 1045: 0.8; FT: 0.9). Predefined thresholds using the scoring system of the ACSS could be established and validated to define \"clinical cure.\" CONCLUSIONS Evaluating not only antibacterial but also nonantibacterial agents indicated for the treatment of AC in women, clinical criteria for diagnostics, and measures of patient-reported outcomes are more important as main objectives than microbiological criteria. In this post hoc evaluation, we showed that the ACSS questionnaire, validated in several languages, has the potential to be used as a suitable instrument for diagnostics and patient-reported outcomes in well-designed, international, clinical studies investigating different treatment modalities of uncomplicated urinary tract infections.", "gemini": "MỤC ĐÍCH Điểm số triệu chứng viêm bàng quang cấp tính (ACSS) được sử dụng trong thử nghiệm lâm sàng so sánh thuốc thảo dược Canephron®N (BNO 1045) với tác nhân kháng khuẩn (fosfomycin trometamol [FT]) trong điều trị viêm bàng quang cấp không biến chứng (AC) ở phụ nữ đã được đánh giá như một biện pháp đo kết quả do bệnh nhân báo cáo trong phân tích hậu nghiên cứu. VẬT LIỆU VÀ PHƯƠNG PHÁP Thử nghiệm không kém hơn, đa trung tâm, ngẫu nhiên, mù đôi, giai đoạn III này được thực hiện tại 51 trung tâm ở Châu Âu. Câu hỏi ACSS được sử dụng để đánh giá mức độ nghiêm trọng và diễn biến của các triệu chứng. KẾT QUẢ Phân tích hậu nghiên cứu bao gồm 325 bệnh nhân được điều trị bằng BNO 1045 và 332 bệnh nhân được điều trị bằng FT (tổng cộng 657 bệnh nhân). Tổng điểm trung bình của phạm vi điển hình của ACSS tương đương nhau giữa các nhóm vào ngày 1 (BNO 1045: 10,2; FT: 10,1), sau đó giảm vào ngày 4 (BNO 1045: 5,1; FT: 4,5), vào cuối điều trị vào ngày 8 (BNO 1045: 2,1; FT: 2,1) và vào thời điểm theo dõi muộn vào ngày 38 (BNO 1045: 0,8; FT: 0,9). Các ngưỡng được xác định trước bằng cách sử dụng hệ thống tính điểm của ACSS có thể được thiết lập và xác nhận để xác định \"chữa khỏi lâm sàng\". KẾT LUẬN Đánh giá không chỉ các tác nhân kháng khuẩn mà còn cả các tác nhân không kháng khuẩn được chỉ định để điều trị AC ở phụ nữ, các tiêu chí lâm sàng để chẩn đoán và các biện pháp đo kết quả do bệnh nhân báo cáo quan trọng hơn như các mục tiêu chính so với các tiêu chí vi sinh. Trong đánh giá hậu nghiên cứu này, chúng tôi đã chỉ ra rằng bảng câu hỏi ACSS, được xác nhận bằng nhiều ngôn ngữ, có khả năng được sử dụng như một công cụ phù hợp để chẩn đoán và kết quả do bệnh nhân báo cáo trong các nghiên cứu lâm sàng quốc tế được thiết kế tốt, điều tra các phương thức điều trị khác nhau của nhiễm trùng đường tiết niệu không biến chứng."} +{"idx": 1870058, "en": "SIGNIFICANCE Extremity injury represents the leading cause of trauma hospitalizations among adults under the age of 65 years, and long-term impairments are often substantial. Restoring function depends, in large part, on bone and soft tissue healing. Thus, decisions around treatment strategy are based on assessment of the healing potential of injured bone and/or soft tissue. However, at the present, this assessment is based on subjective clinical clues and/or cadaveric studies without any objective measure. Optical imaging is an ideal method to solve several of these issues. AIM The aim is to highlight the current challenges in assessing bone and tissue perfusion/viability and the potentially high impact applications for optical imaging in orthopaedic surgery. APPROACH The prospective will review the current challenges faced by the orthopaedic surgeon and briefly discuss optical imaging tools that have been published. With this in mind, it will suggest key research areas that could be evolved to help make surgical assessments more objective and quantitative. RESULTS Orthopaedic surgical procedures should benefit from incorporation of methods to measure functional blood perfusion or tissue metabolism. The types of measurements though can vary in the depth of tissue sampled, with some being quite superficial and others sensing several millimeters into the tissue. Most of these intrasurgical imaging tools represent an ideal way to improve surgical treatment of orthopaedic injuries due to their inherent point-of-care use and their compatibility with real-time management. CONCLUSION While there are several optical measurements to directly measure bone function, the choice of tools can determine also the signal strength and depth of sampling. For orthopaedic surgery, real-time data regarding bone and tissue perfusion should lead to more effective patient-specific management of common orthopaedic conditions, requiring deeper penetrance commonly seen with indocyanine green imaging. This will lower morbidity and result in decreased variability associated with how these conditions are managed.", "gemini": "TẦM QUAN TRỌNG Chấn thương chi là nguyên nhân hàng đầu gây nhập viện chấn thương ở những người trưởng thành dưới 65 tuổi và các khiếm khuyết lâu dài thường rất đáng kể. Việc phục hồi chức năng phụ thuộc phần lớn vào quá trình lành xương và mô mềm. Do đó, các quyết định về chiến lược điều trị dựa trên đánh giá khả năng lành của xương và/hoặc mô mềm bị thương. Tuy nhiên, hiện tại, đánh giá này dựa trên các đầu mối lâm sàng chủ quan và/hoặc các nghiên cứu trên xác chết mà không có bất kỳ biện pháp khách quan nào. Chụp ảnh quang là một phương pháp lý tưởng để giải quyết một số vấn đề này. MỤC ĐÍCH Mục đích là nêu bật những thách thức hiện tại trong việc đánh giá tưới máu/sức sống của xương và mô và các ứng dụng tác động cao tiềm ẩn đối với hình ảnh quang học trong phẫu thuật chỉnh hình. PHƯƠNG PHÁP Triển vọng sẽ xem xét các thách thức hiện tại mà bác sĩ phẫu thuật chỉnh hình phải đối mặt và thảo luận ngắn gọn về các công cụ hình ảnh quang học đã được công bố. Với ý nghĩ này, nó sẽ đề xuất các lĩnh vực nghiên cứu chính có thể được phát triển để giúp đánh giá phẫu thuật khách quan và định lượng hơn. KẾT QUẢ Các quy trình phẫu thuật chỉnh hình sẽ được hưởng lợi từ việc kết hợp các phương pháp để đo lưu lượng máu chức năng hoặc chuyển hóa mô. Tuy nhiên, các loại phép đo có thể thay đổi về độ sâu của mô được lấy mẫu, một số rất nông và một số khác cảm nhận được sâu vài milimet vào mô. Hầu hết các công cụ hình ảnh trong phẫu thuật này đại diện cho một cách lý tưởng để cải thiện điều trị phẫu thuật chấn thương chỉnh hình do chúng vốn có thể sử dụng tại nơi chăm sóc và tương thích với quản lý thời gian thực. KẾT LUẬN Mặc dù có một số phép đo quang học để đo trực tiếp chức năng của xương, nhưng việc lựa chọn các công cụ cũng có thể xác định cường độ tín hiệu và độ sâu lấy mẫu. Đối với phẫu thuật chỉnh hình, dữ liệu thời gian thực liên quan đến tưới máu của xương và mô sẽ dẫn đến việc quản lý hiệu quả hơn tình trạng chỉnh hình phổ biến theo từng bệnh nhân, đòi hỏi độ thâm nhập sâu hơn thường thấy khi chụp ảnh xanh indocyanine. Điều này sẽ làm giảm tỷ lệ mắc bệnh và dẫn đến giảm sự thay đổi liên quan đến cách quản lý các tình trạng này."} +{"idx": 1870059, "en": "OBJECTIVE To observe the effect of electroacupuncture (EA) on liver surface blood perfusion and related indexes of oxidative stress in mice with acute alcoholic liver injury （AALI）, so as to explore its mechanism underlying improving of AALI. METHODS Twenty-four Kunming mice were randomly divided into control, model and EA groups (8 mice/group). AALI model was established by intragastric administration of 50% ethanol at 6 g/kg. EA (4-6 V, 50 Hz) was applied to bilateral\"Zusanli\"(ST36) and\"Taichong\"(LR3) for 30 min. The liver surface blood perfusion was dynamically observed by moorFLPI laser speckle imager for 30 min. The morphological changes of the liver tissue were determined by H.E. staining. Levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the liver were measured by ELISA. RESULTS Compared with the control group, the model group had markedly pathological changes of the hepatic cords, hepatic sinuses and hepatocytes shown by H.E. staining, a significant reduction in liver surface blood perfusion and the content of SOD in liver tissue (P<0.01, P<0.05), and a significant increase in the content of MDA in liver tissue (P<0.05). Compared with the model group, H.E. staining showed reduced pathological changes of the liver tissue, meanwhile the liver surface blood perfusion significant increased (P<0.05) and the content of MDA in liver tissue significantly decreased (P<0.01) in the EA group. CONCLUSION EA can improve the liver surface circulation of AALI mice, and regulate the oxidative stress response, and thus help to repair the damage of liver tissue.", "gemini": "MỤC TIÊU Quan sát tác dụng của điện châm (EA) đối với tưới máu bề mặt gan và các chỉ số liên quan đến stress oxy hóa ở chuột bị tổn thương gan do rượu cấp tính (AALI), nhằm tìm hiểu cơ chế cải thiện AALI của nó. PHƯƠNG PHÁP Hai mươi bốn con chuột Kunming được chia ngẫu nhiên thành nhóm đối chứng, nhóm mô hình và nhóm EA (8 con/nhóm). Mô hình AALI được thiết lập bằng cách dùng gavage uống 50% ethanol với liều 6 g/kg. EA (4-6 V, 50 Hz) được áp dụng cho \"Túc Tam Lý\" (ST36) và \"Thái Xung\" (LR3) ở hai bên trong 30 phút. Tưới máu bề mặt gan được quan sát động bằng máy ảnh chấm laser moorFLPI trong 30 phút. Các thay đổi về mặt hình thái của mô gan được xác định bằng nhuộm H.E. Nồng độ superoxide dismutase (SOD) và malondialdehyde (MDA) trong gan được đo bằng ELISA. KẾT QUẢ So với nhóm đối chứng, nhóm mô hình có những thay đổi bệnh lý rõ rệt ở các dây gan, xoang gan và tế bào gan được thể hiện bằng nhuộm H.E, giảm đáng kể tưới máu bề mặt gan và hàm lượng SOD trong mô gan (P < 0,01, P < 0,05), và tăng đáng kể hàm lượng MDA trong mô gan (P < 0,05). So với nhóm mô hình, nhuộm H.E cho thấy giảm các thay đổi bệnh lý của mô gan, trong khi đó tưới máu bề mặt gan tăng đáng kể (P < 0,05) và hàm lượng MDA trong mô gan giảm đáng kể (P < 0,01) ở nhóm EA. KẾT LUẬN EA có thể cải thiện tuần hoàn bề mặt gan của chuột AALI và điều chỉnh phản ứng stress oxy hóa, do đó giúp phục hồi tổn thương mô gan."} +{"idx": 1870060, "en": "OBJECTIVE To investigate the therapeutic effect of scalp acupuncture and rehabilitation training on balance dysfunction in children with spasmodic hemiplegia so as to provide the reference to the optimization of treatment scheme. METHODS A total of 60 children with spastic hemiplegia were divided into a routine group and a scalp acupuncture group, 30 cases in each one according to random number table. In the routine group, the rehabilitation training was provided, including exercise training, balance training, spasmotherapy apparatus, electromyography biofeedback apparatus and orthoses. In the scalp acupuncture group, on the base of the treatment as the routine group, scalp acupuncture was supplemented at motor area, foot motor sensory area, equilibrium area and parietal temporal anterior oblique line. Separately, before the treatment, after 3 months treatment and after 6 months treatment, the dimension D and E of the gross motor function measure-88 (GMFM-88) and Berg balance scale (BBS) were adopted to evaluate balance related motor functions and equilibrium function. The differences in the above 3 indicators at different time stages were compared in children between the two groups. RESULTS Compared with the score before the treatment, BBS score was obviously increased after 3 and 6 months treatment in the patients of the two groups respectively (P<0.05). The score in the dimension D and E after 6-month treatment was increased significantly as compared with the score before treatment and after 3-month treatment in the same group respectively (P<0.05). Compared with the routine group, the score of dimension D and E of GMFM-88 as well as BBS score were all increased obviously in the scalp acupuncture group after 3 and 6 months treatment (P<0.05). CONCLUSION On the base of routine rehabilitation training, scalp acupuncture can improve balance function of children with spastic hemiplegia better.", "gemini": "MỤC TIÊU Nghiên cứu tác dụng điều trị của châm cứu da đầu và tập phục hồi chức năng đối với rối loạn thăng bằng ở trẻ bại não nửa người co cứng để cung cấp tài liệu tham khảo cho việc tối ưu hóa phác đồ điều trị. PHƯƠNG PHÁP Tổng cộng 60 trẻ bại não nửa người co cứng được chia thành nhóm thường quy và nhóm châm cứu da đầu, mỗi nhóm 30 trường hợp theo bảng số ngẫu nhiên. Ở nhóm thường quy, trẻ được tập phục hồi chức năng, bao gồm tập vận động, tập thăng bằng, máy spa, máy xung điện sinh học cơ và nẹp chỉnh hình. Ở nhóm châm cứu da đầu, trên cơ sở điều trị như nhóm thường quy, trẻ được bổ sung châm cứu da đầu tại vùng vận động, vùng vận động cảm giác bàn chân, vùng thăng bằng và đường xiên trước thái dương đỉnh. Riêng trước khi điều trị, sau 3 tháng điều trị và sau 6 tháng điều trị, sử dụng thang đo chức năng vận động thô-88 (GMFM-88) chiều D và E, thang đánh giá thăng bằng Berg (BBS) để đánh giá chức năng vận động liên quan đến thăng bằng và chức năng thăng bằng. So sánh sự khác biệt về 3 chỉ số trên ở các thời điểm khác nhau giữa hai nhóm trẻ. KẾT QUẢ So với điểm số trước khi điều trị, điểm số BBS sau 3 tháng và 6 tháng điều trị đều tăng rõ rệt ở bệnh nhi của hai nhóm (P < 0,05). Điểm số chiều D và E sau 6 tháng điều trị tăng rõ rệt so với điểm số trước khi điều trị và sau 3 tháng điều trị cùng nhóm (P < 0,05). So với nhóm thường quy, điểm số chiều D và E của GMFM-88 cũng như điểm số BBS đều tăng rõ rệt ở nhóm châm cứu da đầu sau 3 tháng và 6 tháng điều trị (P < 0,05). KẾT LUẬN Trên cơ sở tập phục hồi chức năng thông thường, châm cứu da đầu có thể cải thiện chức năng thăng bằng của trẻ bại não nửa người co cứng tốt hơn."} +{"idx": 1870061, "en": "Chronic atrophic gastritis (CAG) is a common digestive disease in clinic. Previous experimental and clinical studies have shown that acupuncture has a positive effect for CAG. Apoptosis of gastric mucosal tissue has been shown to play an important role in the process of gastric atrophy and possibly further carcinogenesis in CAG, and the circular RNA (circRNA), a novel class of non-coding RNA, has been confirmed to play a regulatory role in the downstream pathway of apoptosis by many stu-dies. Accumulated findings of experimental studies showed that acupuncture and moxibustion interventions could suppress apoptosis of the cultured human gastric mucosal epithelial cells and lower apoptotic index of gastric mucosal cells in CAG rats. Therefore, circRNA is likely to mediate the inhibitory effect of acupuncture and moxibustion on apoptosis of gastric mucosal epithelial cells in CAG. In this paper, we systematically summarized 1) the regulation of circRNA on apoptosis, 2) the apoptosis and pathological mechanism of CAG, 3) the effect of acupuncture on apoptosis, and proposed that circRNA is highly likely to be involved in the positive effect of acupuncture and moxibustion interventions for CAG. It is recommended that researches should further reveal the scientific basis of acupuncture and moxibustion therapies in the treatment of CAG by exploring the role of related circRNAs and their downstream target proteins in the gastric mucosal tissues.", "gemini": "Viêm dạ dày teo mãn tính (CAG) là một bệnh tiêu hóa phổ biến trong lâm sàng. Các nghiên cứu thực nghiệm và lâm sàng trước đây đã chỉ ra rằng châm cứu có tác dụng tích cực đối với CAG. Chứng apoptosis của mô niêm mạc dạ dày đã được chứng minh là đóng một vai trò quan trọng trong quá trình teo dạ dày và có thể gây ung thư trong CAG, và RNA vòng (circRNA), một loại RNA không mã hóa mới, đã được xác nhận là đóng vai trò điều hòa trong con đường hạ lưu của chứng apoptosis bởi nhiều nghiên cứu. Những phát hiện tích lũy của các nghiên cứu thực nghiệm cho thấy các can thiệp châm cứu và cứu ngải có thể ức chế apoptosis của các tế bào biểu mô niêm mạc dạ dày nuôi cấy của người và giảm chỉ số apoptotic của các tế bào niêm mạc dạ dày ở chuột CAG. Do đó, circRNA có khả năng trung gian tác dụng ức chế của châm cứu và cứu ngải đối với chứng apoptosis của các tế bào biểu mô niêm mạc dạ dày trong CAG. Trong bài báo này, chúng tôi đã tóm tắt một cách có hệ thống 1) sự điều hòa của circRNA đối với chứng apoptosis, 2) chứng apoptosis và cơ chế bệnh sinh của CAG, 3) tác dụng của châm cứu đối với chứng apoptosis và đề xuất rằng circRNA rất có thể liên quan đến tác dụng tích cực của các can thiệp châm cứu và cứu ngải đối với CAG. Chúng tôi khuyến cáo rằng các nghiên cứu nên tiếp tục làm sáng tỏ cơ sở khoa học của các liệu pháp châm cứu và cứu ngải trong điều trị CAG bằng cách khám phá vai trò của các circRNA liên quan và các protein đích hạ lưu của chúng trong các mô niêm mạc dạ dày."} +{"idx": 1870062, "en": "Nonspecific low back pain (NLBP) is a heterogeneous disease with no definite pathological anatomical causes. Although acupuncture treatment of NLBP has been included in the international clinical guidelines for low back pain in many countries, the grade strength is still low, and the Results of clinical efficacy evaluation are controversial. Moderate evidence from international studies indicates that acupuncture treatment of NLBP has a clear short-term analgesic effect and can improve pain related depression symptoms to a certain extent, but the exploration of efficacy rules is relatively insufficient, and there is no specific operational scheme to guide the acupuncture treatment of NLBP. Domestic clinical studies are quite distinctive in the aspects of dose-effect acupoint selection, characteristic needling Methods, and acupuncture apparatuses, but lack of systematic research, and have low quality and weak evidence. In terms of the underlying mechanisms, acupuncture may achieve analgesic effect through anti-inflammation, relieving central sensitization, and improving the damage of local multifidus muscles and bones. However, due to the heterogeneity of NLBP disease, the current elaboration on the mechanism of acupuncture treatment of NLBP may not reach a consensus, and it is still worth further exploration.", "gemini": "Đau thắt lưng không đặc hiệu (NLBP) là một bệnh lý không đồng nhất không có nguyên nhân giải phẫu bệnh lý xác định. Mặc dù điều trị NLBP bằng châm cứu đã được đưa vào hướng dẫn lâm sàng quốc tế về đau thắt lưng ở nhiều quốc gia, nhưng mức độ phân loại vẫn còn thấp và Kết quả đánh giá hiệu quả lâm sàng còn nhiều tranh cãi. Bằng chứng vừa phải từ các nghiên cứu quốc tế chỉ ra rằng điều trị NLBP bằng châm cứu có tác dụng giảm đau rõ rệt trong thời gian ngắn và có thể cải thiện các triệu chứng trầm cảm liên quan đến đau ở một mức độ nhất định, nhưng việc khám phá các quy tắc hiệu quả còn tương đối chưa đủ và không có phác đồ cụ thể để hướng dẫn điều trị NLBP bằng châm cứu. Các nghiên cứu lâm sàng trong nước khá đặc biệt về các khía cạnh lựa chọn huyệt đạo liều-tác dụng, Phương pháp châm cứu đặc trưng và dụng cụ châm cứu, nhưng thiếu nghiên cứu có hệ thống và có chất lượng thấp và bằng chứng yếu. Về cơ chế tiềm ẩn, châm cứu có thể đạt được hiệu quả giảm đau thông qua chống viêm, giảm nhạy cảm trung ương và cải thiện tổn thương của cơ đa đốt sống và xương tại chỗ. Tuy nhiên, do tính không đồng nhất của bệnh NLBP, nên việc giải thích hiện tại về cơ chế điều trị NLBP bằng châm cứu có thể chưa đạt được sự đồng thuận và vẫn đáng được khám phá thêm."} +{"idx": 1870063, "en": "Histological pictures are important parts of medical research articles, and a good histological picture not only visualizes the content of the article, but also makes it easier for readers to understand. With the rapid development of microscopic technology and image processing technology, journals have higher requirements for the quality of histological pictures and more strict requirements for the editing and annotation of pictures. Based on an analysis of the current status of histological pictures in the articles of Acupuncture Research, this article proposes our suggestions and Methods for rational use of histological pictures, so as to provide a reference for improving the quality of histological pictures in the articles published in Acupuncture Research.", "gemini": "Các hình ảnh mô học là một phần quan trọng của các bài báo nghiên cứu y học, một hình ảnh mô học tốt không chỉ trực quan hóa nội dung của bài viết mà còn giúp người đọc dễ hiểu hơn. Với sự phát triển nhanh chóng của công nghệ kính hiển vi và công nghệ xử lý hình ảnh, các tạp chí có yêu cầu cao hơn về chất lượng hình ảnh mô học và yêu cầu nghiêm ngặt hơn đối với việc chỉnh sửa và chú thích hình ảnh. Trên cơ sở phân tích tình trạng hiện tại của các hình ảnh mô học trong các bài báo của Nghiên cứu Châm cứu, bài viết này đề xuất các đề xuất và Phương pháp của chúng tôi để sử dụng hợp lý các hình ảnh mô học, để cung cấp tài liệu tham khảo để nâng cao chất lượng hình ảnh mô học trong các bài báo được xuất bản trên Nghiên cứu Châm cứu."} +{"idx": 1870064, "en": "BACKGROUND Emergency departments (EDs) in New Zealand are experiencing growing demand because of rising attendances, and this is having a negative effect on patients, staff and organisations. The expansion of traditional nursing roles is one solution that has been explored internationally to ameliorate the adverse effects of increasing patient attendances. AIM To explore the attitudes of registered nurses and physicians employed in emergency medicine towards an expanded role for registered nurses in the ED setting. METHOD A mixed-methods approach was adopted, in which data were collected in two phases, using interviews and questionnaires. Semi-structured, face-to-face interviews were held with clinical and non-clinical staff employed in emergency medicine, and questionnaires were distributed to 140 physicians and nurses employed in one ED. The data were analysed to identify themes and to determine the differences between nurse and physician respondents. FINDINGS Ten ED staff were interviewed, including non-clinical managers (n=4), physicians (n=2) and nurses (n=4). Analysis of the interviews identified five themes: driving change; expanded nurse role; optimal environment; facilitating change; and optimising outcomes for patients. A total of 70 questionnaires were returned, with 63 from nurses and seven from physicians. Nurse respondents were more supportive than their physician colleagues of the need to expand the nursing role in the ED. CONCLUSION The existing New Zealand nursing scope of practice has the capacity to develop roles, with nurses supporting this change more than physicians. If role expansion is undertaken, serious consideration needs to be given to developing clear professional boundaries to maintain patient safety and department flow.", "gemini": "HOÀN CẢNH Các khoa cấp cứu (ED) ở New Zealand đang phải đối mặt với nhu cầu ngày càng tăng do lượng người tham gia tăng cao, và điều này đang gây ảnh hưởng tiêu cực đến bệnh nhân, nhân viên và các tổ chức. Việc mở rộng các vai trò điều dưỡng truyền thống là một giải pháp đã được khám phá trên toàn thế giới để cải thiện các tác động tiêu cực của việc tăng lượng bệnh nhân tham gia. MỤC ĐÍCH Khảo sát thái độ của các y tá và bác sĩ đã đăng ký làm việc trong lĩnh vực y học cấp cứu đối với vai trò mở rộng của các y tá đã đăng ký trong môi trường ED. PHƯƠNG PHÁP Một phương pháp hỗn hợp đã được áp dụng, trong đó dữ liệu được thu thập trong hai giai đoạn, sử dụng các cuộc phỏng vấn và bảng câu hỏi. Các cuộc phỏng vấn trực tiếp, có cấu trúc bán phần đã được thực hiện với nhân viên lâm sàng và phi lâm sàng làm việc trong lĩnh vực y học cấp cứu và các bảng câu hỏi đã được phân phối cho 140 bác sĩ và y tá làm việc tại một khoa cấp cứu. Dữ liệu đã được phân tích để xác định các chủ đề và xác định sự khác biệt giữa các đối tượng trả lời là y tá và bác sĩ. KẾT QUẢ Mười nhân viên ED đã được phỏng vấn, bao gồm cả những người quản lý phi lâm sàng (n = 4), bác sĩ (n = 2) và y tá (n = 4). Phân tích các cuộc phỏng vấn đã xác định được năm chủ đề: thúc đẩy thay đổi; vai trò của y tá được mở rộng; môi trường tối ưu; tạo điều kiện thay đổi; và tối ưu hóa kết quả cho bệnh nhân. Tổng cộng có 70 bảng câu hỏi được trả lại, trong đó có 63 bảng từ y tá và bảy bảng từ bác sĩ. Những người trả lời là y tá ủng hộ nhiều hơn so với các đồng nghiệp bác sĩ của họ về nhu cầu mở rộng vai trò điều dưỡng trong khoa cấp cứu. KẾT LUẬN Phạm vi hành nghề điều dưỡng hiện tại của New Zealand có khả năng phát triển các vai trò, với các y tá ủng hộ sự thay đổi này nhiều hơn các bác sĩ. Nếu mở rộng vai trò, cần cân nhắc nghiêm túc đến việc phát triển các ranh giới chuyên môn rõ ràng để duy trì sự an toàn của bệnh nhân và luồng công việc của khoa."} +{"idx": 1870065, "en": "The effect of acupuncture-moxibustion on respiratory system and systemic immune inflammatory response were reviewed to explore the possible role of neuroimmunomodulation in the control of inflammatory response and the effect mechanism of cholinergic anti-inflammatory pathway on coronavirus disease 2019 (COVID-19). Acupuncture-moxibustion could produce the local and systemic anti-inflammatory effect on COVID-19 through the activation of cholinergic anti-inflammatory pathway. Compared with humoral anti-inflammatory pathway, the neuronal anti-inflammatory pathway has earlier initiation, rapider action, and more localization, which play a more important role in the initial stage of inflammatory response. This may be an important basis for acupuncture-moxibustion intervention in the early stage of COVID-19. In addition to cholinergic anti-inflammatory pathway, acupuncture-moxibustion may also play an anti-inflammatory role in activating sympathetic nerve, hypothalamic-pituitary-adrenal axis and other neural anti-inflammatory pathways. How acupuncture-moxibustion play its role in stimulating the vagus nerve and sympathetic nerve in different periods of inflammatory response, and whether the effect is based on the selection of acupoints and the methods of stimulation, will be the research direction of the transformation from basic research to clinical research for acupuncture-moxibustion.", "gemini": "Tác dụng của châm cứu-cứu trên hệ hô hấp và đáp ứng viêm miễn dịch toàn thân đã được xem xét để khám phá vai trò có thể có của điều hòa miễn dịch thần kinh trong việc kiểm soát đáp ứng viêm và cơ chế tác dụng của con đường chống viêm cholinergic trên bệnh do vi-rút corona 2019 (COVID-19). Châm cứu-cứu có thể tạo ra tác dụng chống viêm tại chỗ và toàn thân đối với COVID-19 thông qua việc kích hoạt con đường chống viêm cholinergic. So với con đường chống viêm dịch thể, con đường chống viêm thần kinh có khởi phát sớm hơn, tác dụng nhanh hơn và khu trú hơn, đóng vai trò quan trọng hơn trong giai đoạn đầu của đáp ứng viêm. Đây có thể là cơ sở quan trọng để can thiệp châm cứu-cứu trong giai đoạn đầu của COVID-19. Ngoài con đường chống viêm cholinergic, châm cứu-cứu cũng có thể đóng vai trò chống viêm trong việc kích hoạt dây thần kinh giao cảm, trục hạ đồi-tuyến yên-thượng thận và các con đường chống viêm thần kinh khác. Làm thế nào châm cứu-cứu đóng vai trò của mình trong việc kích thích dây thần kinh phế vị và dây thần kinh giao cảm trong các giai đoạn khác nhau của đáp ứng viêm, và liệu hiệu quả có dựa trên sự lựa chọn huyệt đạo và các phương pháp kích thích hay không, sẽ là hướng nghiên cứu chuyển đổi từ nghiên cứu cơ bản sang nghiên cứu lâm sàng về châm cứu-cứu."} +{"idx": 1870066, "en": "OBJECTIVE To evaluate the clinical effect of acupuncture at tendon blockage point of quadriceps femoris muscle belly for mild to moderate patella femoral arthritis. METHODS A total of 76 patients with mild to moderate patella femoral arthritis were randomly divided into an observation group and a control group, 38 cases in each group. The patients in the observation group were treated with acupuncture at tendon blockage point of quadriceps femoris muscle belly, and the needles were stayed for 20 min each time for twice a week; while the patients in the control group were treated with sodium hyaluronate injection into articular cavity, once a week, and both groups were treated for 4 weeks. The pain symptoms and joint function of the patients were evaluated with pain visual analogue scale (VAS) score, tenderness value of the most obvious pain point in front of the knee, and Lysholm knee function score before and one week after treatment, and the clinical effect was observed. RESULTS After treatment, VAS scores, tenderness value of the most obvious pain point in front of the knee and Lysholm knee function scores of the two groups were improved compared with before treatment (P<0.05); the improvement of VAS score and tenderness value of the most obvious pain point in front of the knee in the observation group was more obvious than that in the control group (P<0.05). The total effective rate in the observation group was 94.7% (36/38), which was higher than 81.6% (31/38) in the control group (P<0.05). CONCLUSION Acupuncture at tendon blockage point of quadriceps femoris muscle belly can relieve pain and improve the function of patella femoral joint for patients with patella femoral arthritis, and the clinical effect is better than that of sodium hyaluronate injected into articular cavity.", "gemini": "MỤC TIÊU Đánh giá hiệu quả lâm sàng của châm cứu tại điểm tắc gân của cơ tứ đầu đùi trong viêm khớp xương bánh chè từ nhẹ đến trung bình. PHƯƠNG PHÁP Tổng cộng 76 bệnh nhân bị viêm khớp xương bánh chè từ nhẹ đến trung bình được chia ngẫu nhiên thành nhóm quan sát và nhóm đối chứng, mỗi nhóm 38 ca. Bệnh nhân trong nhóm quan sát được điều trị bằng châm cứu tại điểm tắc gân của cơ tứ đầu đùi, châm giữ kim 20 phút mỗi lần, mỗi tuần châm 2 lần; bệnh nhân trong nhóm đối chứng được tiêm natri hyaluronat vào khoang khớp, mỗi tuần tiêm 1 lần, cả hai nhóm đều được điều trị trong 4 tuần. Đánh giá triệu chứng đau và chức năng khớp của bệnh nhân bằng thang điểm tương tự thị giác đau (VAS), giá trị đau của điểm đau rõ nhất trước gối và thang điểm chức năng Lysholm trước và một tuần sau điều trị, quan sát hiệu quả lâm sàng. KẾT QUẢ Sau điều trị, điểm VAS, giá trị đau của điểm đau rõ nhất trước gối và thang điểm chức năng Lysholm của hai nhóm đều được cải thiện so với trước điều trị (P < 0,05); sự cải thiện về điểm VAS và giá trị đau của điểm đau rõ nhất trước gối ở nhóm quan sát rõ ràng hơn so với nhóm đối chứng (P < 0,05). Tỷ lệ có hiệu quả tổng thể ở nhóm quan sát là 94,7% (36/38), cao hơn 81,6% (31/38) ở nhóm đối chứng (P < 0,05). KẾT LUẬN Châm cứu tại điểm tắc gân của cơ tứ đầu đùi có thể giảm đau và cải thiện chức năng khớp xương bánh chè cho bệnh nhân viêm khớp xương bánh chè, hiệu quả lâm sàng tốt hơn so với tiêm natri hyaluronat vào khoang khớp."} +{"idx": 1870067, "en": "To introduce professor ZHANG Ren's experience that different stages of refractory facial paralysis should be treated with different acupuncture methods.In early stage of facial paralysis,identifcation is important. Electroacupuncture is applied to connect Qianzheng (Extra) with Cuanzhu (BL 2), Sibai (ST 2) and Jiachengjiang (Extra), by observing the twitches of facial muscles, it is determined whether it is easy to develop into refractory facial paralysis, in order to actively take preventive treatment. In the recovery stage of refractory facial paralysis, comprehensive therapy including acupuncture, acupoint injection, quick cupping and auricular point pressure are adopted, and penetration needling is applied at three points of mouth, three points of cheek and three points of forehead.In the sequelae stage of refractory facial paralysis，on the basis of comprehensive therapy, targeted treatment is adopted according to different sequelae.", "gemini": "Giới thiệu kinh nghiệm của giáo sư ZHANG Ren rằng các giai đoạn khác nhau của liệt mặt kháng trị nên được điều trị bằng các phương pháp châm cứu khác nhau. Ở giai đoạn đầu của liệt mặt, việc xác định là rất quan trọng. Điện châm được áp dụng để kết nối Qianzheng (Ngoại) với Cuanzhu (BL 2), Sibai (ST 2) và Jiachengjiang (Ngoại), bằng cách quan sát các cơn co giật của cơ mặt, xác định xem có dễ phát triển thành liệt mặt kháng trị hay không, để chủ động thực hiện điều trị dự phòng. Trong giai đoạn phục hồi của liệt mặt kháng trị, áp dụng liệu pháp toàn diện bao gồm châm cứu, tiêm huyệt, giác hơi nhanh và ấn huyệt, đồng thời châm kim xuyên tại ba điểm của miệng, ba điểm của má và ba điểm của trán. Trong giai đoạn di chứng của liệt mặt kháng trị, trên cơ sở của liệu pháp toàn diện, áp dụng điều trị có mục tiêu theo các di chứng khác nhau."} +{"idx": 1870068, "en": "In view of the five major misunderstandings in the clinical understanding of gastroesophageal reflux disease in traditional Chinese medicine, that is, wrong main symptoms, wrong position of the disease, wrong character of the disease, treating acid with acid, and attaching importance to drugs and neglecting acupuncture-moxibustion in treatment, Professor BAI Xing-hua has proposed the corresponding solving strategies, which includes grasping the main disease, carefully examining the disease position, distinguishing the character of disease, and no acid suppression for acid regurgitation. In addition, Professor BAI highly values the external treatment of traditional Chinese medicine. In particular, the acupuncture therapy of \"Tongdu Jiangni (promote the circulation of the governor vessel and reduce the reversed qi)\" based on many years of clinical experience provides new ideas and methods for the diagnosis and treatment of gastroesophageal reflux disease in traditional Chinese medicine.", "gemini": "Trước năm hiểu lầm lớn trong hiểu biết lâm sàng về bệnh trào ngược dạ dày thực quản trong y học cổ truyền, tức là triệu chứng chính sai, vị trí bệnh sai, tính chất bệnh sai, chữa chua bằng chua, coi trọng thuốc mà bỏ qua châm cứu trong điều trị, Giáo sư BAI Xing-hua đã đề xuất các chiến lược giải quyết tương ứng, bao gồm nắm bắt bệnh chính, kiểm tra cẩn thận vị trí bệnh, phân biệt tính chất bệnh và không ức chế axit đối với chứng trào ngược axit. Ngoài ra, Giáo sư BAI rất coi trọng việc điều trị bên ngoài của y học cổ truyền. Đặc biệt, liệu pháp châm cứu \"Thông đốc giang nghịch (thông kinh đốc và giảm nghịch khí)\" dựa trên nhiều năm kinh nghiệm lâm sàng cung cấp những ý tưởng và phương pháp mới cho chẩn đoán và điều trị bệnh trào ngược dạ dày thực quản trong y học cổ truyền."} +{"idx": 1870069, "en": "The creative viewpoint of SHENG Xie-sun, the master of Zhejiang school of Chinese medicine is introduced regarding the theories of acupuncture-moxibustion and acupoints. It is stated by master SHENG that the five-tissue needling methods are applicable not only for physical disorders, but also for zangfu disorders. Of these methods, the nearby bone needling method presents an instructive significance in treatment of pain disorder. The \"reinforcing in the upper and reducing in the lower\" needling method supplements the reinforcing and reducing theory of acupuncture. Master SHENG has put forward the theory of flexible selection of acupoint, the phenomenon of acupoint groups and the application of transverse acupoint composition, as well as the measures for intensifying needling induction, promoting qi regulation of acupuncture and improving clinical effect. Besides, the seven Chinese characters are used to summarize acupoint composition, meaning \"main point, supplementary point, nearby point, corresponding point, back-shu point, front-mu point and extra point\" separately. This creative viewpoint provides a clearer and more feasible mode in treatment with acupuncture-moxibustion and is instructive proactively in clinical practice.", "gemini": "Giới thiệu quan điểm sáng tạo của Thánh Tế Tôn, bậc thầy của trường phái y học Trung Quốc Chiết Giang về các lý thuyết châm cứu - cứu và huyệt vị. Thánh Tế Tôn nêu rõ rằng các phương pháp châm cứu ngũ tạng không chỉ áp dụng cho các rối loạn về thể chất mà còn cho các rối loạn về tạng phủ. Trong số các phương pháp này, phương pháp châm cứu gần xương có ý nghĩa hướng dẫn trong điều trị rối loạn đau. Phương pháp châm cứu \"bổ thượng tả hạ\" bổ sung cho lý thuyết bổ tả của châm cứu. Thánh Tế Tôn đã đưa ra lý thuyết về việc lựa chọn huyệt vị linh hoạt, hiện tượng nhóm huyệt vị và ứng dụng của thành phần huyệt vị ngang, cũng như các biện pháp tăng cường cảm ứng châm cứu, thúc đẩy điều hòa khí của châm cứu và cải thiện hiệu quả lâm sàng. Bên cạnh đó, bảy ký tự Hán được sử dụng để tóm tắt thành phần huyệt vị, có nghĩa là \"huyệt chính, huyệt bổ sung, huyệt gần, huyệt tương ứng, huyệt lưng, huyệt trước và huyệt ngoài\" riêng biệt. Quan điểm sáng tạo này cung cấp một phương thức điều trị rõ ràng hơn và khả thi hơn bằng châm cứu - cứu và có tính hướng dẫn chủ động trong thực hành lâm sàng."} +{"idx": 1870070, "en": "Started from the needs of clinical teaching and practice of acupuncture and moxibustion, based on the acupuncture Tongren education and assessment model, the virtual acupuncture teaching system was developed with the help of virtual reality (VR) technology, and applied to the course teaching of meridian and acupoint and needling and moxibustion method of Acupuncture Sciences. Compared with conventional teaching, this system can effectively improve practical operation test scores of students, meanwhile, it has higher interest, interactivity and helpfulness for knowledge learning, and improve independent learning ability, learning effect and memory depth, so student's satisfaction is higher.", "gemini": "Xuất phát từ nhu cầu giảng dạy và thực hành lâm sàng về châm cứu và cứu, dựa trên mô hình đào tạo và đánh giá giáo dục châm cứu Đồng Nhân, hệ thống giảng dạy châm cứu ảo đã được phát triển với sự trợ giúp của công nghệ thực tế ảo (VR), và áp dụng vào giảng dạy môn kinh lạc và huyệt vị và phương pháp châm cứu cứu của ngành Khoa học Châm cứu. So với phương pháp giảng dạy thông thường, hệ thống này có thể cải thiện hiệu quả điểm kiểm tra vận hành thực hành của sinh viên, đồng thời có hứng thú, tính tương tác và tính hữu ích cao hơn cho việc học kiến thức, đồng thời cải thiện khả năng tự học, hiệu quả học tập và độ sâu ghi nhớ, do đó sự hài lòng của sinh viên cao hơn."} +{"idx": 1870071, "en": "This paper analyzes the problems existing in the current theory of divergent meridian (\"Jingbie\"), such as the unclear route of divergent meridian, the controversy in the \"li-he-chu-ru\" and the lack of in-depth research on the theory of \"liuhe\", clarifies the source and privides insights. The author analyzed the word \"Jingbie\" by means of the four proofreading methods combined with philology method to explore the origin of \"Jingbie \". The divergent meridians is the remnant of the original meridians system in the bamboo and silk documents, which was put into the meridians system after being sorted out by doctors. The twelve meridians are combined in two, forming the \"liuhe\" point, which is located in the neck, and has a great influence on the blood circulation of the head and neck. \"liuhe\" point and theory can guide the differentiation of acupuncture and moxibustion, and has an important guiding significance for the treatment of empirical, emergency and head and face diseases.", "gemini": "Bài báo này phân tích các vấn đề tồn tại trong học thuyết kinh lạc kỳ kinh (\"Kinh biệt\") hiện nay như lộ trình kinh lạc kỳ kinh chưa rõ ràng, tranh cãi trong \"lý hợp chủ nhập\" và thiếu nghiên cứu chuyên sâu về học thuyết \"lục hợp\", làm rõ nguồn gốc và đưa ra những hiểu biết sâu sắc. Tác giả đã phân tích từ \"Kinh biệt\" bằng phương pháp đối chiếu bốn lần kết hợp với phương pháp ngữ văn để tìm hiểu nguồn gốc của \"Kinh biệt\". Kinh lạc kỳ kinh là tàn dư của hệ thống kinh lạc ban đầu trong các tài liệu bằng tre và lụa, được đưa vào hệ thống kinh lạc sau khi được các thầy thuốc phân loại. Mười hai kinh mạch kết hợp thành hai, tạo thành điểm \"lục hợp\", nằm ở cổ, có ảnh hưởng lớn đến lưu thông máu ở đầu và cổ. Điểm \"lục hợp\" và học thuyết có thể hướng dẫn phân biệt châm cứu và cứu, có ý nghĩa chỉ đạo quan trọng đối với việc điều trị các bệnh theo kinh nghiệm, cấp cứu và các bệnh về đầu mặt."} +{"idx": 1870072, "en": "With the discussion on the origins and the evolution of Star Twelve Points, combined with ancient astronomical stellar map, it is realized that the three-dimensional spatial diagram of Star Twelve Points can be approximately regarded as a Big Dipper map from the side view. Under the direction of image thinking, the function of Big Dipper was compared with the function of Star Twelve Points. Furthermore, according to \"the opening-closing-pivoting\" theory in The Inner Canon of Huangdi and the theory of \"qi cycle in round\" proposed by HUANG Yuan-yu, the mechanisms of Star Twelve Points on adjusting functional activities of qi and the movement of viscera-meridian-qi-blood is elaborated, providing a new idea for acupuncture clinical treatment of miscellaneous diseases.", "gemini": "Với sự thảo luận về nguồn gốc và sự phát triển của Thập Nhị Tinh, kết hợp với bản đồ sao thiên văn cổ đại, người ta nhận ra rằng sơ đồ không gian ba chiều của Thập Nhị Tinh có thể được coi gần đúng là bản đồ chòm sao Đại Hùng từ góc nhìn bên. Dưới sự chỉ đạo của tư duy hình ảnh, chức năng của chòm sao Đại Hùng đã được so sánh với chức năng của Thập Nhị Tinh. Hơn nữa, theo lý thuyết \"khai-bế-trục\" trong Nội kinh của Hoàng đế và lý thuyết \"khí tuần hoàn\" do HUANG Yuan-yu đề xuất, các cơ chế của Thập Nhị Tinh trong việc điều chỉnh hoạt động chức năng của khí và sự vận động của kinh lạc-khí-máu được giải thích, cung cấp một ý tưởng mới cho điều trị lâm sàng bằng châm cứu các bệnh tạp."} +{"idx": 1870073, "en": "Acupoint selection rules of neurogenic dysphagia treated with acupuncture and moxibustion from pre-Qin to late Qing Dynasty in Chinese Medical Classics were analyzed based on data mining. The literature regarding acupuncture and moxibustion for neurogenic dysphagia was searched and screened according to the inclusion and exclusion criteria in Chinese Medical Classics (5th Edition), the prescriptions were extracted according to the principle of acupoint extraction.The SPSS 21.0 and Clementine 12.0 were used to perform the cluster analysis and association rule analysis.A total of 191 acupuncture and moxibustion prescriptions were screened and extracted，including 45 acupoints. The top 5 acupoints of acupuncture and moxibustion for neurogenic dysphagia in frequency were Jiache (ST 6), Dicang (ST 4), Lieque (LU 7), Lianquan (CV 23), Shuigou (GV 26). The most involved meridians were the stomach meridian, the governor vessel and the conception vessel. The main acupoints were distributed in the scalp face neck and upper limbs. The most frequently used specific acupoints was crossing points,next was eight confluence points. There were 11 acupoint combinations with strong association according to the association rule analysis, and the top one acupoint combination was Hegu (LI 4)-Jiache (ST 6). There were 9 acupoint cluster groups according to the cluster analysis. Hegu (LI 4), Jiache (ST 6), Dicang (ST 4), Shuigou (GV 26) and Chengjiang (CV 24) were core acupoints for neurogenic dysphagia treated with acupuncture and moxibustion in ancient times, besides，selecting proximal and distal acupoints and selecting acupoints according to symptoms were emphasized.", "gemini": "Các quy tắc chọn huyệt của chứng khó nuốt do thần kinh được điều trị bằng châm cứu và cứu từ thời tiền Tần đến cuối thời nhà Thanh trong Y học cổ truyền Trung Quốc đã được phân tích dựa trên khai thác dữ liệu. Các tài liệu về châm cứu và cứu đối với chứng khó nuốt do thần kinh đã được tìm kiếm và sàng lọc theo các tiêu chuẩn đưa vào và loại trừ trong Y học cổ truyền Trung Quốc (Phiên bản thứ 5), các đơn thuốc đã được trích xuất theo nguyên tắc trích xuất huyệt. SPSS 21.0 và Clementine 12.0 đã được sử dụng để thực hiện phân tích cụm và phân tích quy tắc liên kết. Tổng cộng 191 đơn thuốc châm cứu và cứu đã được sàng lọc và trích xuất, bao gồm 45 huyệt. 5 huyệt hàng đầu về châm cứu và cứu đối với chứng khó nuốt do thần kinh theo tần suất là Giáp xa (ST 6), Địa thương (ST 4), Liệt khuyết (LU 7), Liên quyền (CV 23), Thủy câu (GV 26). Các kinh mạch liên quan nhiều nhất là kinh vị, kinh đốc và kinh nhiệm mạch. Các huyệt chính được phân bố ở vùng da đầu mặt cổ và chi trên. Các huyệt cụ thể được sử dụng thường xuyên nhất là các huyệt giao nhau, tiếp theo là tám huyệt giao hội. Có 11 tổ hợp huyệt có liên kết mạnh theo phân tích quy tắc liên kết và tổ hợp huyệt hàng đầu là Hợp cốc (LI 4) - Giáp xa (ST 6). Có 9 nhóm cụm huyệt theo phân tích cụm. Hợp cốc (LI 4), Giáp xa (ST 6), Địa thương (ST 4), Thủy câu (GV 26) và Thành cường (CV 24) là các huyệt cốt lõi đối với chứng khó nuốt do thần kinh được điều trị bằng châm cứu và cứu trong thời cổ đại, ngoài ra, việc lựa chọn các huyệt gần và xa và lựa chọn huyệt theo các triệu chứng đã được nhấn mạnh."} +{"idx": 1870074, "en": "The rule and characteristics of clinical acupoint selection were explored in treatment of puerperal insufficient lactation with acupuncture and moxibustion. The clinical articles on the treatment of puerperal insufficient lactation with acupuncture and moxibustion were retrieved from the databases of CNKI, VIP and Wanfang from the date of establishment to June 1, 2019. The articles were screened in accordance with the inclusion and exclusion criteria. The prescriptions of acupuncture and moxibustion were extracted and normalized. Using Microsoft Excel 2016 software, the use frequency, meridians involved and acupoint distributions were analyzed. Using SPSS Statistics 21.0 software, the cluster and factor analyses were conducted. Totally, 102 articles were included,108 acupoint prescriptions were extracted, 65 acupoitns were designed and the total use frequency was 654 times. The top three acupoints used in treatment of puerperal insufficient lactation were Danzhong (CV 17), Rugen (ST 18) and Shaoze (SI 1). The most frequently involved meridians were the stomach meridian, the conception vessel, the small intestine meridian and the liver meridian. The acupoints were mainly distributed in the chest and four extremities. It was showed in cluster analysis that Rugen (ST 18), Shaoze (SI 1), Zusanli (ST 36) and Danzhong (CV 17) represent 3 clusters and a total of 7 common factors were extracted. The acupoint selection is based on syndrome differentiation in treatment of puerperal insufficient lactation with acupuncture and moxibustion, of which, the local acupoints are predominated and the distal acupoints are combined.", "gemini": "Quy luật và đặc điểm của việc lựa chọn huyệt lâm sàng đã được khám phá trong điều trị chứng ít sữa sau sinh bằng châm cứu và cứu. Các bài báo lâm sàng về điều trị chứng ít sữa sau sinh bằng châm cứu và cứu đã được lấy từ các cơ sở dữ liệu của CNKI, VIP và Wanfang từ ngày thành lập đến ngày 1 tháng 6 năm 2019. Các bài báo đã được sàng lọc theo các tiêu chuẩn đưa vào và loại trừ. Các đơn thuốc châm cứu và cứu đã được trích xuất và chuẩn hóa. Sử dụng phần mềm Microsoft Excel 2016, tần suất sử dụng, kinh mạch liên quan và phân bố huyệt đã được phân tích. Sử dụng phần mềm SPSS Statistics 21.0, phân tích cụm và phân tích nhân tố đã được tiến hành. Tổng cộng, 102 bài báo đã được đưa vào, 108 đơn thuốc huyệt đã được trích xuất, 65 huyệt được thiết kế và tổng tần suất sử dụng là 654 lần. Ba huyệt hàng đầu được sử dụng trong điều trị chứng ít sữa sau sinh là Đản trung (CV 17), Nhũ căn (ST 18) và Thiếu trạch (SI 1). Các kinh mạch liên quan thường xuyên nhất là kinh vị, kinh nhiệm mạch, kinh tiểu trường và kinh can. Các huyệt chủ yếu phân bố ở ngực và bốn chi. Phân tích cụm cho thấy Nhũ căn (ST 18), Thiếu trạch (SI 1), Túc tam lý (ST 36) và Đản trung (CV 17) đại diện cho 3 cụm và tổng cộng 7 yếu tố chung đã được trích xuất. Việc lựa chọn huyệt dựa trên phân biệt hội chứng trong điều trị chứng ít sữa sau sinh bằng châm cứu và cứu, trong đó, các huyệt tại chỗ chiếm ưu thế và các huyệt xa được kết hợp."} +{"idx": 1870075, "en": "OBJECTIVE To evaluate the reporting quality of randomized controlled trials (RCTs) of acupuncture for vascular dementia. METHODS The RCTs of acupuncture for vascular dementia were systematically retrieved from the Cochrane Library, PubMed, EMbase, CNKI, SinoMed, VIP and Wanfang databases from the date of establishment to October 31, 2018. The reporting quality of RCT was evaluated based on the internationally-recognized Consolidated Standards for Reporting of Trials (CONSORT) statement and Standards for Reporting Interventions in Controlled Trials of Acupuncture (STRICTA). RESULTS A total of 33 RCTs were included. According to the CONSORT statement, 12 items had a reporting rate of 0%, including important changes to methods after trial commencement, sample size, blind method, trial registry, etc.; 5 items had a reporting rate of below 10%, including trial design, type of randomization, random allocation sequence, randomization implementation and participant flow. The reporting rate was 36.36% for baseline data and 57.58% for randomization sequence generation. According to the STRICTA statement, the reporting rate was 21.21% for description of acupuncture depth, 60.61% for description of acupuncture response, 27.27% for description of additional intervention details and 0% for qualification of acupuncturists, respectively. CONCLUSION At present, the reporting quality of RCTs of acupuncture for vascular dementia is generally low. In the future, the RCTs of acupuncture for vascular dementia should be normatively reported according to the CONSORT statement and STRICTA statement.", "gemini": "MỤC TIÊU Đánh giá chất lượng báo cáo của các thử nghiệm ngẫu nhiên có đối chứng (RCT) về châm cứu cho chứng sa sút trí tuệ mạch máu. PHƯƠNG PHÁP Các RCT về châm cứu cho chứng sa sút trí tuệ mạch máu đã được lấy một cách có hệ thống từ các cơ sở dữ liệu Cochrane Library, PubMed, EMbase, CNKI, SinoMed, VIP và Wanfang từ ngày thành lập đến ngày 31 tháng 10 năm 2018. Chất lượng báo cáo của RCT đã được đánh giá dựa trên tuyên bố Chuẩn mực báo cáo thử nghiệm được công nhận quốc tế (CONSORT) và Chuẩn mực báo cáo can thiệp trong các thử nghiệm có đối chứng về châm cứu (STRICTA). KẾT QUẢ Tổng cộng có 33 RCT được đưa vào. Theo tuyên bố CONSORT, 12 mục có tỷ lệ báo cáo là 0%, bao gồm những thay đổi quan trọng đối với các phương pháp sau khi bắt đầu thử nghiệm, quy mô mẫu, phương pháp mù, sổ đăng ký thử nghiệm, v.v.; 5 mục có tỷ lệ báo cáo dưới 10%, bao gồm thiết kế thử nghiệm, loại phân ngẫu nhiên, trình tự phân bổ ngẫu nhiên, triển khai phân ngẫu nhiên và dòng người tham gia. Tỷ lệ báo cáo là 36,36% đối với dữ liệu ban đầu và 57,58% đối với việc tạo chuỗi ngẫu nhiên. Theo tuyên bố STRICTA, tỷ lệ báo cáo lần lượt là 21,21% đối với mô tả độ sâu châm cứu, 60,61% đối với mô tả phản ứng châm cứu, 27,27% đối với mô tả các chi tiết can thiệp bổ sung và 0% đối với trình độ của người châm cứu. KẾT LUẬN Hiện nay, chất lượng báo cáo của các RCT về châm cứu cho chứng sa sút trí tuệ mạch máu nhìn chung còn thấp. Trong tương lai, các RCT về châm cứu cho chứng sa sút trí tuệ mạch máu cần được báo cáo theo chuẩn mực theo tuyên bố CONSORT và tuyên bố STRICTA."} +{"idx": 1870076, "en": "The research progress of acupuncture analgesia in recent years is analyzed to summarize the analgesic mechanism of acupuncture on neuropathic pain. The analgesic mechanism of acupuncture on neuropathic pain is discussed from peripheral level and central level, including peripheral sensitization and immune inflammatory response, changes of ion channel, central sensitization, regulation of cell signal pathway, activation of spinal glial cells, etc. It is suggested that the focus of future research should include conducting in-vitro studies with the help of multi-omics technology to detect the changes of metabolic substances and signal pathway molecules in patients with neuropathic pain before and after acupuncture to further clarify the mechanism of acupuncture analgesia.", "gemini": "Phân tích tiến trình nghiên cứu về giảm đau bằng châm cứu trong những năm gần đây để tóm tắt cơ chế giảm đau của châm cứu đối với cơn đau thần kinh. Cơ chế giảm đau của châm cứu đối với cơn đau thần kinh được thảo luận từ cấp độ ngoại vi và cấp độ trung ương, bao gồm nhạy cảm ngoại vi và đáp ứng viêm miễn dịch, thay đổi kênh ion, nhạy cảm trung ương, điều chỉnh con đường tín hiệu tế bào, kích hoạt tế bào thần kinh đệm tủy sống, v.v. Người ta cho rằng trọng tâm của nghiên cứu trong tương lai nên bao gồm việc tiến hành các nghiên cứu trong ống nghiệm với sự trợ giúp của công nghệ đa ô học để phát hiện những thay đổi về chất chuyển hóa và phân tử con đường tín hiệu ở những bệnh nhân đau thần kinh trước và sau khi châm cứu để làm rõ thêm cơ chế giảm đau bằng châm cứu."} +{"idx": 1870077, "en": "Despite well recognized improvements in obesity-related comorbidities, increasing evidence implicates bariatric surgery in the onset of adverse skeletal health outcomes. The purpose of this review is to provide a focused update in three critical areas: (i) emergent data on sleeve gastrectomy and bone loss, (ii) evidence linking bariatric surgery to incident fracture, and (iii) intervention strategies designed to mitigate surgical bone loss. Better understanding of these issues will inform our treatment of skeletal health for patients planning bariatric surgery.", "gemini": "Mặc dù có những cải thiện được công nhận về các bệnh đi kèm liên quan đến béo phì, ngày càng có nhiều bằng chứng liên quan đến phẫu thuật giảm béo trong việc khởi phát các kết cục sức khỏe xương bất lợi. Mục đích của bài đánh giá này là cung cấp bản cập nhật tập trung vào ba lĩnh vực quan trọng: (i) dữ liệu mới nổi về cắt dạ dày tay áo và mất xương, (ii) bằng chứng liên kết phẫu thuật giảm béo với gãy xương và (iii) các chiến lược can thiệp được thiết kế để giảm thiểu mất xương do phẫu thuật. Hiểu rõ hơn về các vấn đề này sẽ cung cấp thông tin cho việc điều trị sức khỏe xương của chúng tôi cho những bệnh nhân có kế hoạch phẫu thuật giảm béo."} +{"idx": 1870078, "en": "OBJECTIVE To improve treatment outcomes in patients with acute appendicitis (AA). MATERIAL AND METHODS An internet survey was performed. Questionnaire consisted of 15 questions concerning diagnosis and treatment of AA: application of prognostic scales, incidence and technical aspects of laparoscopic appendectomy (LA), antibiotic prophylaxis, postoperative management, compliance with international and national clinical guidelines. A total of 690 questionnaires were received and analyzed (3.67% of all surgeons in the Russian Federation). RESULTS Eighteen percent of respondents use at least one prognostic scale. The vast majority of surgeons (92%) use antibiotic prophylaxis. Almost half of respondents place trocars in the triangulation position (44%), one third of surgeons ligate the mesentery of the appendix (35%), most respondents perform mesoappendectomy (60%) with monopolar and bipolar cautery. Forty-five percent of all respondents do not invert the appendix stump. Significant number of respondents use abdominal drainage routinely. Only 3.5% of surgeons use multimodal postoperative analgesia. Less than 22% of patients are operated under low-pressure pneumoperitoneum. Standardization of surgical technique and perioperative approaches including those specified in the guidelines is absent. We also found insufficient awareness of surgeons about international and national clinical guidelines. CONCLUSION This study may be useful for standardizing treatment approaches, choosing the best practice, popularizing and improving of current clinical guidelines.", "gemini": "MỤC TIÊU Cải thiện kết quả điều trị ở những bệnh nhân bị viêm ruột thừa cấp tính (AA). VẬT LIỆU VÀ PHƯƠNG PHÁP Một cuộc khảo sát trên internet đã được thực hiện. Bản câu hỏi bao gồm 15 câu hỏi liên quan đến chẩn đoán và điều trị AA: ứng dụng các thang đánh giá tiên lượng, tỷ lệ mắc và các khía cạnh kỹ thuật của cắt ruột thừa nội soi (LA), dự phòng kháng sinh, quản lý hậu phẫu, tuân thủ các hướng dẫn lâm sàng quốc tế và quốc gia. Tổng cộng có 690 bảng câu hỏi đã được tiếp nhận và phân tích (3,67% trong số tất cả các bác sĩ phẫu thuật ở Liên bang Nga). KẾT QUẢ Mười tám phần trăm số người được hỏi sử dụng ít nhất một thang đánh giá tiên lượng. Phần lớn các bác sĩ phẫu thuật (92%) sử dụng dự phòng kháng sinh. Gần một nửa số người được hỏi đặt trocar ở vị trí tam giác (44%), một phần ba số bác sĩ phẫu thuật thắt mạc treo ruột thừa (35%), hầu hết những người được hỏi thực hiện cắt mạc treo ruột thừa (60%) bằng dao đốt đơn cực và lưỡng cực. Bốn mươi lăm phần trăm trong số tất cả những người được hỏi không lộn gốc ruột thừa. Một số lượng đáng kể những người được hỏi sử dụng dẫn lưu ổ bụng thường quy. Chỉ có 3,5% bác sĩ phẫu thuật sử dụng giảm đau đa phương thức sau phẫu thuật. Ít hơn 22% bệnh nhân được phẫu thuật dưới áp lực thấp trong ổ bụng. Không có sự chuẩn hóa kỹ thuật phẫu thuật và các phương pháp phẫu thuật trước và trong khi phẫu thuật, bao gồm cả những phương pháp được chỉ định trong các hướng dẫn. Chúng tôi cũng thấy rằng các bác sĩ phẫu thuật không nhận thức đầy đủ về các hướng dẫn lâm sàng quốc tế và quốc gia. KẾT LUẬN Nghiên cứu này có thể hữu ích cho việc chuẩn hóa các phương pháp điều trị, lựa chọn phương pháp thực hành tốt nhất, phổ biến và cải thiện các hướng dẫn lâm sàng hiện hành."} +{"idx": 1870079, "en": "OBJECTIVE To evaluate the effectiveness of surgical treatment of post-burn defects of face and cranial vault. MATERIAL AND METHODS There were 65 patients with post-burn defects of face and cranial vault. Mean age of patients was 38.5 years (min 17 years, max 67 years). Soft tissue reconstruction was performed by using of local tissues, combined plasty, balloon dermotension aand free flaps on microvascular anastomoses. Osteoectomy and sequestrectomy were carried out if it was necessary. Implants were used to eliminate through skull defects. RESULTS Balloon dermotension with expanders and plasty with local tissues ensure skull reconstruction in the vast majority of patients. Through bone defects required reconstruction of the cranial vault with various implants. CONCLUSION The proposed surgical approach ensures correction of severe functional and cosmetic disorders caused by post-burn skull defects, elimination of bone damage, restoration of normal skin of the face and cranial vault.", "gemini": "MỤC TIÊU Đánh giá hiệu quả của điều trị phẫu thuật các khiếm khuyết do bỏng ở mặt và vòm sọ. VẬT LIỆU VÀ PHƯƠNG PHÁP Có 65 bệnh nhân bị khiếm khuyết do bỏng ở mặt và vòm sọ. Độ tuổi trung bình của bệnh nhân là 38,5 tuổi (tối thiểu 17 tuổi, tối đa 67 tuổi). Tái tạo mô mềm được thực hiện bằng cách sử dụng các mô tại chỗ, tạo hình kết hợp, tạo bóng da bằng bóng và các vạt tự do trên các nối vi phẫu. Cắt xương và cắt bỏ xương chết đã được thực hiện nếu cần thiết. Các miếng ghép được sử dụng để loại bỏ các khiếm khuyết của hộp sọ. KẾT QUẢ Tạo bóng da bằng bóng với dụng cụ mở rộng và tạo hình bằng các mô tại chỗ đảm bảo tái tạo hộp sọ ở phần lớn bệnh nhân. Các khiếm khuyết của xương cần tái tạo vòm sọ bằng các miếng ghép khác nhau. KẾT LUẬN Phương pháp phẫu thuật được đề xuất đảm bảo sửa chữa các rối loạn chức năng và thẩm mỹ nghiêm trọng do các khiếm khuyết của hộp sọ do bỏng, loại bỏ tổn thương xương, phục hồi làn da bình thường của mặt và vòm sọ."} +{"idx": 1870080, "en": "OBJECTIVE To identify the criteria of smooth postoperative period after cholecystectomy and risk factors of local surgical infection. MATERIAL AND METHODS A prospective analysis included 680 patients after laparoscopic cholecystectomy. We assessed incidence and risk factors of infectious complications. Major markers of acute inflammation, ultrasonic features of surgical site in early postoperative period were analyzed. RESULTS Postoperative infectious complications developed in 35 patients (5.1%). All studied risk factors except an age (obesity, cardiorespiratory diseases, surgery time over 150 min, violation of the principles of antibiotic therapy, intraoperative blood loss over 50 ml, drainage time over 5 days) significantly affected the development of infectious complications. Surgery time over 150 min and violation of the principles of antibiotic therapy were the most significant factors. Serum procalcitonin, erythrocyte sedimentation rate and C-reactive protein were characterized by the highest prognostic value on the 3rd postoperative day. Threshold values were defined. CONCLUSION Surgery time and violation of the rules of antibiotic prophylaxis were the most significant risk factors of postoperative infectious complications. We determined the criteria of smooth postoperative period: procalcitonin <1.5 mg/l, C-reactive protein <50 mg/l, erythrocyte sedimentation rate <39 mm/h, ultrasonic pattern of hypoechoic accumulation within the bed of the bladder (dimension <10 mm) without signs of intestinal insufficiency, abdominal or subhepatic effusion on the 3rd day after surgery.", "gemini": "MỤC TIÊU Xác định các tiêu chí của giai đoạn hậu phẫu suôn sẻ sau cắt túi mật và các yếu tố nguy cơ nhiễm trùng phẫu thuật tại chỗ. VẬT LIỆU VÀ PHƯƠNG PHÁP Phân tích triển vọng bao gồm 680 bệnh nhân sau cắt túi mật nội soi. Chúng tôi đánh giá tỷ lệ mắc và các yếu tố nguy cơ của các biến chứng nhiễm trùng. Các dấu hiệu chính của tình trạng viêm cấp tính, các đặc điểm siêu âm của vị trí phẫu thuật trong giai đoạn hậu phẫu sớm đã được phân tích. KẾT QUẢ Các biến chứng nhiễm trùng sau phẫu thuật phát triển ở 35 bệnh nhân (5,1%). Tất cả các yếu tố nguy cơ được nghiên cứu ngoại trừ tuổi (béo phì, bệnh tim mạch hô hấp, thời gian phẫu thuật trên 150 phút, vi phạm các nguyên tắc điều trị bằng kháng sinh, mất máu trong mổ trên 50 ml, thời gian dẫn lưu trên 5 ngày) đều ảnh hưởng đáng kể đến sự phát triển của các biến chứng nhiễm trùng. Thời gian phẫu thuật trên 150 phút và vi phạm các nguyên tắc điều trị bằng kháng sinh là những yếu tố quan trọng nhất. Procalcitonin huyết thanh, tốc độ lắng hồng cầu và protein phản ứng C được đặc trưng bởi giá trị tiên lượng cao nhất vào ngày hậu phẫu thứ 3. Các giá trị ngưỡng đã được xác định. KẾT LUẬN Thời gian phẫu thuật và vi phạm các quy tắc dự phòng kháng sinh là những yếu tố nguy cơ quan trọng nhất của các biến chứng nhiễm trùng sau phẫu thuật. Chúng tôi xác định các tiêu chí của giai đoạn hậu phẫu suôn sẻ: procalcitonin <1,5 mg/l, protein phản ứng C <50 mg/l, tốc độ lắng hồng cầu <39 mm/h, kiểu siêu âm của sự tích tụ giảm âm trong lòng bàng quang (kích thước <10 mm) mà không có dấu hiệu suy chức năng ruột, tràn dịch ổ bụng hoặc dưới gan vào ngày thứ 3 sau phẫu thuật."} +{"idx": 1870081, "en": "The aim of this study was to observe the expression of Klotho in renal tissues of mice with diabetic ne¬phropathy (DN), and to further explore the effect of Klotho on DN in mice and its mechanism. The 10-week-old mice in this experiment were divided into three groups: heterozygous db/+ mouse group (db/+ group, n=20), homozygous db/db mouse group (db/db group, n=20) and homozygous db/db mice + Klotho group (db/db + Klotho group, n=20). Firstly, Western blotting and immunohistochemical staining were applied to detect the protein expression of Klotho in the renal tissues of diabetic and non-diabetic mice of different ages. Finally, the protein expressions of fibroblast growth factor 2 (FGF2) and E-cadherin in the renal tissues of mice in each group were examined by Western blotting. The protein expression level of Klotho in the renal tissues of mice aged 10 and 16 weeks in the db/db group was remarkably lower than that in yhedb/+ group. In addition, it was found that db/db + Klotho group exhibited a prominently lower degree of interstitial fi¬brosis and content of Collagen I and Collagen III in the renal tissues than db/db group. Furthermore, it was revealed that the overexpression of Klotho could significantly repress the protein expression level of FGF2 but elevate that of E-cadherin in the renal tissues of DN mice. Klotho protein may ameliorate the renal injury and fibrosis in diabetic mice by inhibiting FGF2, so it is expected to become a targeted drug for DN.", "gemini": "Mục tiêu của nghiên cứu này là quan sát biểu hiện của Klotho trong mô thận của chuột bị bệnh thận do đái tháo đường (DN) và khám phá thêm tác dụng của Klotho đối với DN ở chuột và cơ chế của nó. Chuột 10 tuần tuổi trong thí nghiệm này được chia thành ba nhóm: nhóm chuột db/+ dị hợp tử (nhóm db/+, n=20), nhóm chuột db/db đồng hợp tử (nhóm db/db, n=20) và nhóm chuột db/db đồng hợp tử + nhóm Klotho (nhóm db/db + Klotho, n=20). Đầu tiên, phương pháp blotting Western và nhuộm miễn dịch mô miễn dịch đã được áp dụng để phát hiện biểu hiện protein của Klotho trong mô thận của chuột đái tháo đường và không đái tháo đường ở các độ tuổi khác nhau. Cuối cùng, biểu hiện protein của yếu tố tăng trưởng nguyên bào sợi 2 (FGF2) và E-cadherin trong mô thận của chuột ở mỗi nhóm được kiểm tra bằng phương pháp blotting Western. Mức độ biểu hiện protein của Klotho trong mô thận của chuột ở tuổi 10 và 16 tuần ở nhóm db/db thấp hơn đáng kể so với nhóm yhedb/+. Ngoài ra, người ta thấy rằng nhóm db/db + Klotho biểu hiện mức độ xơ kẽ và hàm lượng Collagen I và Collagen III trong mô thận thấp hơn đáng kể so với nhóm db/db. Hơn nữa, người ta phát hiện ra rằng sự biểu hiện quá mức của Klotho có thể ức chế đáng kể mức độ biểu hiện protein của FGF2 nhưng lại làm tăng mức độ biểu hiện của E-cadherin trong mô thận của chuột DN. Protein Klotho có thể cải thiện tình trạng tổn thương thận và xơ hóa ở chuột đái tháo đường bằng cách ức chế FGF2, vì vậy nó được kỳ vọng sẽ trở thành một loại thuốc đích cho DN."} +{"idx": 1870082, "en": "OBJECTIVE Optimization of diagnosis and treatment of patients with solitary fibrous tumor of pleura, analysis of overall survival and disease-free survival, predictors of recurrence. MATERIAL AND METHODS There were 66 patients with solitary fibrous tumor of pleura (26 men and 40 women) aged 57.6 years (range 26-80 years). Asymptomatic course was found in 29 (44%) patients, various symptoms - in 37 (56%) patients. Thoracotomy was applied in 36 patients, thoracoscopy - in 30 patients. Immunohistochemical examination included analysis of definition of Stat6 expression. RESULTS Benign variant of SFT was diagnosed in 50 (75.7%) patients, malignant variant - in 16 (24.3%) patients. STAT6 expression was observed in all cases. Postoperative morbidity was 9%, mortality - 1.6%. Recurrence was diagnosed in 2 (4%) patients with benign variant of disease and in 5 (31.2%) patients with malignant variant (2 of them died from progression of disease). Progression-free survival was 89.4%, overall survival - 95.4%. Predictors of recurrence are tumor dimension over 10 cm, necrosis and/or hemorrhagic component of tumor, mitotic count of at least four per 10 high-power fields. CONCLUSION. S Olitary fibrous tumor of pleura is a rare mesenchymal fibroblastic neoplasm growing from submesothelial layer. Differential and preoperative morphological diagnosis of SFT is difficult and demands a special immunohistochemical examination with analysis of Stat 6 expression. Surgery is preferred for tumor de novo and recurrent neoplasm.", "gemini": "MỤC TIÊU Tối ưu hóa chẩn đoán và điều trị cho bệnh nhân bị u xơ đơn độc màng phổi, phân tích tỷ lệ sống chung và không có bệnh, yếu tố dự báo tái phát. VẬT LIỆU VÀ PHƯƠNG PHÁP Có 66 bệnh nhân bị u xơ đơn độc màng phổi (26 nam và 40 nữ) tuổi trung bình 57,6 (dao động 26-80 tuổi). Diễn biến không triệu chứng được tìm thấy ở 29 (44%) bệnh nhân, các triệu chứng khác nhau - ở 37 (56%) bệnh nhân. Phẫu thuật mở ngực được áp dụng cho 36 bệnh nhân, nội soi lồng ngực - ở 30 bệnh nhân. Kiểm tra miễn dịch mô học bao gồm phân tích định nghĩa biểu hiện Stat6. KẾT QUẢ Biến thể lành tính của SFT được chẩn đoán ở 50 (75,7%) bệnh nhân, biến thể ác tính - ở 16 (24,3%) bệnh nhân. Biểu hiện STAT6 được quan sát thấy trong tất cả các trường hợp. Tỷ lệ mắc bệnh sau phẫu thuật là 9%, tỷ lệ tử vong - 1,6%. Tái phát được chẩn đoán ở 2 (4%) bệnh nhân có biến thể lành tính của bệnh và ở 5 (31,2%) bệnh nhân có biến thể ác tính (2 trong số họ tử vong do bệnh tiến triển). Tỷ lệ sống không tiến triển là 89,4%, tỷ lệ sống chung là 95,4%. Các yếu tố dự báo tái phát là kích thước khối u trên 10 cm, hoại tử và/hoặc thành phần xuất huyết của khối u, số lượng nguyên phân ít nhất bốn trên 10 trường quan sát độ phóng đại cao. KẾT LUẬN. U xơ đơn độc màng phổi là một khối u sợi trung mô hiếm gặp phát triển từ lớp dưới màng phổi. Chẩn đoán hình thái học phân biệt và trước phẫu thuật của SFT rất khó khăn và đòi hỏi phải có xét nghiệm miễn dịch mô học đặc biệt với phân tích biểu hiện Stat 6. Phẫu thuật được ưu tiên cho khối u de novo và khối u tái phát."} +{"idx": 1870083, "en": "OBJECTIVE To compare various approaches to revascularization of aortoiliac-femoral segment. MATERIAL AND METHODS Data were collected prospectively for retrospective analysis. There were 192 patients with atherosclerotic lesion of the aortoiliac-femoral segment who underwent reconstructive surgeries. All patients were divided into 3 groups depending on the type of reconstruction: 85 patients underwent open surgical interventions, 63 patients - endovascular interventions, 44 patients - hybrid techniques. Between-group differences were considered significant at p-value <0.05. RESULTS Hybrid revascularization is characterized by less duration of surgery, blood loss and morbidity. Hybrid interventions ensured favorable primary patency compared to open surgery within the follow-up period. CONCLUSION Hybrid revascularization of aortoiliac-femoral segment is characterized by less duration of surgery, blood loss and morbidity.", "gemini": "MỤC TIÊU So sánh các phương pháp khác nhau để tái thông đoạn chủ chậu-đùi. VẬT LIỆU VÀ PHƯƠNG PHÁP Dữ liệu được thu thập có chủ đích để phân tích hồi cứu. Có 192 bệnh nhân bị tổn thương xơ vữa động mạch đoạn chủ chậu-đùi đã được phẫu thuật tái tạo. Tất cả bệnh nhân được chia thành 3 nhóm tùy thuộc vào loại tái tạo: 85 bệnh nhân được can thiệp phẫu thuật mở, 63 bệnh nhân - can thiệp nội mạch, 44 bệnh nhân - kỹ thuật lai. Sự khác biệt giữa các nhóm được coi là có ý nghĩa khi giá trị p < 0,05. KẾT QUẢ Tái thông lai được đặc trưng bởi thời gian phẫu thuật, mất máu và tỷ lệ mắc bệnh thấp hơn. Các can thiệp lai đảm bảo thông suốt ban đầu thuận lợi hơn so với phẫu thuật mở trong thời gian theo dõi. KẾT LUẬN Tái thông lai đoạn chủ chậu-đùi được đặc trưng bởi thời gian phẫu thuật, mất máu và tỷ lệ mắc bệnh thấp hơn."} +{"idx": 1870084, "en": "OBJECTIVE To study the immediate results of therapy and indirect revascularization in patients with critical ischemia of the lower limbs. MATERIAL AND METHODS The results of medication and surgical treatment were analyzed in 210 patients with critical ischemia of the lower limbs. Atherosclerosis obliterans was diagnosed in 142 patients, thromboangiitis obliterans - in 68 patients. Lesion of femoropopliteal segment was observed in 32 (15.2%) cases, popliteal-tibial segment - 68 (32.8%) patients, tibial and foot segment - 62 (29.5%) cases, foot - 31 (14.8%) cases, multiple-level lesion - 17 (8.1%) cases. Survey consisted of Doppler ultrasound, CT angiography, rheovasography with analysis of rheographic index (RI) and pulse oximetry. Circulatory parameters were compared with identical values in 48 almost healthy persons (\"reference group\"). The results of medication and surgical treatment were evaluated by using of the scale of Rutherford R.B. et al. (1997). RESULTS Conservative treatment was performed in 48 patients (control group). The following types of indirect revascularization operations were performed to stimulate regional circulation: bone trepanation in 42 patients, lumbar sympathectomy in 51 patients, bone trepanation + lumbar sympathectomy in 38 patients, bone trepanation with intraosseous irradiation in 31 cases. CONCLUSION Indirect revascularization improves early postoperative outcomes, ensures maintaining support function of the limb and active lifestyle in patients with critical ischemia of the lower limbs. Technical simplicity of these procedures facilitates widespread introduction of indirect revascularization in multi-field hospitals.", "gemini": "MỤC ĐÍCH Nghiên cứu kết quả tức thời của liệu pháp và tái tưới máu gián tiếp ở những bệnh nhân bị thiếu máu cục bộ chi dưới. PHƯƠNG PHÁP VÀ VẬT LIỆU Các kết quả điều trị nội khoa và ngoại khoa được phân tích ở 210 bệnh nhân bị thiếu máu cục bộ chi dưới. Xơ vữa động mạch được chẩn đoán ở 142 bệnh nhân, viêm tắc mạch máu huyết khối - ở 68 bệnh nhân. Tổn thương đoạn đùi-bắp chân được quan sát thấy ở 32 (15,2%) trường hợp, đoạn khoeo-cẳng chân - 68 (32,8%) bệnh nhân, đoạn cẳng chân và bàn chân - 62 (29,5%) trường hợp, bàn chân - 31 (14,8%) trường hợp, tổn thương nhiều mức độ - 17 (8,1%) trường hợp. Khảo sát bao gồm siêu âm Doppler, chụp mạch CT, lưu ký mạch máu với phân tích chỉ số lưu ký mạch máu (RI) và đo oxy xung. Các thông số tuần hoàn được so sánh với các giá trị tương tự ở 48 người gần như khỏe mạnh (\"nhóm tham chiếu\"). Kết quả điều trị nội khoa và ngoại khoa được đánh giá bằng cách sử dụng thang điểm của Rutherford R.B. và cộng sự (1997). KẾT QUẢ Điều trị bảo tồn được thực hiện ở 48 bệnh nhân (nhóm đối chứng). Các loại phẫu thuật tái tưới máu gián tiếp sau đây được thực hiện để kích thích tuần hoàn khu vực: khoan xương ở 42 bệnh nhân, cắt thần kinh giao cảm thắt lưng ở 51 bệnh nhân, khoan xương + cắt thần kinh giao cảm thắt lưng ở 38 bệnh nhân, khoan xương với chiếu xạ nội tủy ở 31 trường hợp. KẾT LUẬN Tái tưới máu gián tiếp cải thiện kết quả sau phẫu thuật sớm, đảm bảo duy trì chức năng nâng đỡ của chi và lối sống năng động ở những bệnh nhân bị thiếu máu cục bộ chi dưới. Kỹ thuật đơn giản của các thủ thuật này tạo điều kiện thuận lợi cho việc áp dụng rộng rãi tái tưới máu gián tiếp tại các bệnh viện đa khoa."} +{"idx": 1870085, "en": "OBJECTIVE To analyze an opinion of surgeons on advisability, efficacy and choice of topical hemostatic agents. MATERIAL AND METHODS The research was based on anonymous questioning of surgeons. The questionnaires were developed by using of interactive Google Forms tool (https://www.google.ru/forms). Distribution of questionnaires and invitation of respondents to participation in research were carried out through the Internet. RESULTS The study enrolled 135 surgeons. Colleagues with considerable experience and length of surgical practice prevailed: 10-15 years - 16 people; 15-20 years - 22 people; over 20 years - 38 people. Opinion of less experienced surgeons was also considered: less than 5 years - 40 people; 5-10 years - 19 people. General surgeons prevailed (49%). Hemostatic sutures (30%) and application of topical hemostatic agents (27%) were the most common methods to stop parenchymal bleeding. Efficacy of local hemostatics is confirmed by small number of cases of recurrent intraoperative bleeding (n=24). Thirty-four respondents reported recurrent bleeding after hemostasis by conventional methods. Postoperative suppuration of hemostatic implant was noted by 9.5% of respondents. Most respondents (77%) prefer combination of hemostatic implants and other traditional methods of hemostasis. CONCLUSION According to surgeons' opinion, local hemostatic agents are effective for parenchymal bleeding. This method may be used as basic (injury grade I by E. Moore classification) or additional method of hemostasis (grade I-III). Many respondents consider that application of topical hemostatic agent after imposing hemostatic sutures ensures the greatest efficiency of hemostasis. Moreover, placement of the agent over or under sutures seems to be the most advisable.", "gemini": "MỤC ĐÍCH Phân tích quan điểm của các bác sĩ phẫu thuật về tính khả thi, hiệu quả và lựa chọn các tác nhân cầm máu tại chỗ. PHƯƠNG PHÁP VÀ VẬT LIỆU Nghiên cứu dựa trên việc hỏi ý kiến ẩn danh của các bác sĩ phẫu thuật. Các bảng câu hỏi được phát triển bằng cách sử dụng công cụ Google Forms tương tác (https://www.google.ru/forms). Việc phân phối bảng câu hỏi và lời mời tham gia nghiên cứu được thực hiện qua Internet. KẾT QUẢ Nghiên cứu đã có sự tham gia của 135 bác sĩ phẫu thuật. Những đồng nghiệp có nhiều kinh nghiệm và thời gian hành nghề phẫu thuật chiếm ưu thế: 10-15 năm - 16 người; 15-20 năm - 22 người; trên 20 năm - 38 người. Ý kiến của các bác sĩ phẫu thuật ít kinh nghiệm hơn cũng được xem xét: dưới 5 năm - 40 người; 5-10 năm - 19 người. Bác sĩ phẫu thuật tổng quát chiếm ưu thế (49%). Chỉ khâu cầm máu (30%) và sử dụng các tác nhân cầm máu tại chỗ (27%) là những phương pháp phổ biến nhất để cầm máu nhu mô. Hiệu quả của thuốc cầm máu tại chỗ được xác nhận bởi số lượng nhỏ các trường hợp chảy máu trong mổ tái phát (n=24). Ba mươi bốn người được hỏi báo cáo chảy máu tái phát sau khi cầm máu bằng các phương pháp thông thường. Chảy mủ sau phẫu thuật của vật liệu cấy ghép cầm máu được 9,5% người được hỏi ghi nhận. Hầu hết những người được hỏi (77%) thích kết hợp vật liệu cấy ghép cầm máu và các phương pháp cầm máu truyền thống khác. KẾT LUẬN Theo ý kiến của các bác sĩ phẫu thuật, các tác nhân cầm máu tại chỗ có hiệu quả đối với chảy máu nhu mô. Phương pháp này có thể được sử dụng như phương pháp cơ bản (chấn thương độ I theo phân loại của E. Moore) hoặc phương pháp cầm máu bổ sung (độ I-III). Nhiều người được hỏi cho rằng việc sử dụng tác nhân cầm máu tại chỗ sau khi khâu cầm máu đảm bảo hiệu quả cầm máu cao nhất. Hơn nữa, việc đặt tác nhân trên hoặc dưới chỉ khâu dường như là hợp lý nhất."} +{"idx": 1870086, "en": "OBJECTIVE To substantiate the advisability of using cytoflavin to correct postoperative cognitive dysfunction (POCD) in patients undergoing video laparoscopic cholecystectomy (VLCE) under conditions of inhalation anesthesia with sevoflurane. MATERIAL AND METHODS The data of two representative groups of patients (n=60) who underwent video-laparoscopic cholecystectomy under the conditions of inhalation anesthesia with sevoflurane were analyzed. At the stages of the perioperative period, in order to monitor the state of higher mental functions, neuropsychological testing was performed: anxiety and depression scales (HADS), the Montreal scale of cognitive dysfunction (MoCA), and frontal dysfunction batteries (FAB). Patients of the first group (n=19) were not corrected for POKD. For the correction of cognitive impairment, patients of the second group (n=41) were treated with Cytoflavin according to the 20 ml regimen per 250 ml of 0.9% sodium chloride solution intravenously once before the operation, then within 4 days of the postoperative period. RESULTS Neuropsychological testing in group I patients revealed the development of moderate POCD. The inclusion of Cytoflavin in the treatment regimen in the II group of patients contributed to an improvement in the state of HMF, accompanied by a decrease in the level of anxiety and depression. CONCLUSION The inclusion of Cytoflavin in treatment regimens helps prevent the development of POKD and is accompanied by an improvement in the state of higher mental functions, which is manifested by a decrease in the level of anxiety and depression, favorably affecting the emotional background of patients.", "gemini": "MỤC ĐÍCH Chứng minh tính khả thi của việc sử dụng cytoflavin để điều chỉnh rối loạn chức năng nhận thức sau phẫu thuật (POCD) ở những bệnh nhân trải qua cắt túi mật nội soi bằng video (VLCE) trong điều kiện gây mê bằng hơi với sevoflurane. PHƯƠNG PHÁP VÀ VẬT LIỆU Dữ liệu của hai nhóm bệnh nhân đại diện (n=60) đã trải qua cắt túi mật nội soi bằng video trong điều kiện gây mê bằng hơi với sevoflurane đã được phân tích. Ở các giai đoạn của thời kỳ quanh phẫu thuật, để theo dõi tình trạng chức năng tinh thần cao hơn, đã tiến hành kiểm tra tâm lý thần kinh: thang lo âu và trầm cảm (HADS), thang rối loạn chức năng nhận thức Montreal (MoCA) và bộ pin rối loạn chức năng trán (FAB). Bệnh nhân của nhóm thứ nhất (n=19) không được điều chỉnh POKD. Để điều chỉnh suy giảm nhận thức, bệnh nhân của nhóm thứ hai (n=41) được điều trị bằng Cytoflavin theo chế độ 20 ml trên 250 ml dung dịch natri clorua 0,9% tiêm tĩnh mạch một lần trước khi phẫu thuật, sau đó trong vòng 4 ngày sau phẫu thuật. KẾT QUẢ Kiểm tra tâm lý thần kinh ở những bệnh nhân nhóm I cho thấy sự phát triển của POCD vừa phải. Việc đưa Cytoflavin vào chế độ điều trị ở nhóm bệnh nhân II góp phần cải thiện tình trạng HMF, đi kèm với việc giảm mức độ lo âu và trầm cảm. KẾT LUẬN Việc đưa Cytoflavin vào chế độ điều trị giúp ngăn ngừa sự phát triển của POKD và đi kèm với sự cải thiện tình trạng chức năng tinh thần cao hơn, biểu hiện là giảm mức độ lo âu và trầm cảm, ảnh hưởng thuận lợi đến nền tảng cảm xúc của bệnh nhân."} +{"idx": 1870087, "en": "Treatment of patients with ventral hernias remains one of the most pressing problems of abdominal surgery. Surgeons are trying to find a «gold standard» for the treatment of this pathology. Great hopes are placed on minimally invasive techniques, however, due to their high cost, they do not yet find mass distribution in everyday practice. In our opinion, this is short-sighted. We tried to analyze the feasibility of using minimally invasive techniques in the treatment of patients with ventral hernias of various locations, from the position of clinical and economic efficiency.", "gemini": "Điều trị bệnh nhân bị thoát vị thành bụng vẫn là một trong những vấn đề cấp bách nhất của phẫu thuật bụng. Các bác sĩ phẫu thuật đang cố gắng tìm ra một «tiêu chuẩn vàng» để điều trị bệnh lý này. Kỹ thuật xâm lấn tối thiểu được đặt nhiều kỳ vọng, tuy nhiên, do chi phí cao nên chúng vẫn chưa được phân phối rộng rãi trong thực hành hàng ngày. Theo chúng tôi, đây là sự thiển cận. Chúng tôi đã cố gắng phân tích tính khả thi của việc sử dụng các kỹ thuật xâm lấn tối thiểu trong điều trị bệnh nhân bị thoát vị thành bụng ở nhiều vị trí khác nhau, từ vị trí hiệu quả về mặt lâm sàng và kinh tế."} +{"idx": 1870088, "en": "OBJECTIVE Systematic review and meta-analysis of data on C-reactive protein (CRP) as a predictor of anastomotic leakage (AL) after surgery for colorectal cancer. MATERIAL AND METHODS Literature searching was performed in Medline, Elibrary, Scopus, Web of Science databases. Literature request consisted of keywords «CRP», «colorectal surgery», «anastomotic leakage» for the period 2008-2018. Meta-analysis included 2 manuscripts for the second postoperative day, 7 articles for the third postoperative day and 6 articles for the fourth postoperative day. ROC-analysis was made to determine optimal prognostic values. RESULTS ROC-curve for the second postoperative day - AUC 0.758; optimal CRP value - 154 mg/l (sensitivity 70.1%, specificity 55.6%), 95% confidence interval 0.698-0.819. ROC-curve for the third postoperative day - AUC 0.715; optimal CRP value - 144.5 mg/l (sensitivity 79.1% specificity 60.3%), 95% confidence interval 0.68-0.75. ROC-curve for the fourth postoperative day - AUC 0.767; optimal CRP value - 122.91 mg/l (sensitivity 72.3% specificity 60%), 95% confidence interval 0.73-0.804. CONCLUSION Increased CRP is an early predictor of AL after surgery for colorectal cancer. CRP level ≥144.5 mg/l on the third postoperative day can predict AL (sensitivity 79%, specificity 60%).", "gemini": "MỤC TIÊU Đánh giá có hệ thống và phân tích tổng hợp dữ liệu về protein phản ứng C (CRP) như một yếu tố dự báo rò nối (AL) sau phẫu thuật ung thư đại trực tràng. VẬT LIỆU VÀ PHƯƠNG PHÁP Tìm kiếm tài liệu được thực hiện trong các cơ sở dữ liệu Medline, Elibrary, Scopus, Web of Science. Yêu cầu tài liệu bao gồm các từ khóa «CRP», «phẫu thuật đại trực tràng», «rò nối» trong giai đoạn 2008-2018. Phân tích tổng hợp bao gồm 2 bản thảo cho ngày hậu phẫu thứ hai, 7 bài báo cho ngày hậu phẫu thứ ba và 6 bài báo cho ngày hậu phẫu thứ tư. Phân tích ROC được thực hiện để xác định các giá trị tiên lượng tối ưu. KẾT QUẢ Đường cong ROC cho ngày hậu phẫu thứ hai - AUC 0,758; giá trị CRP tối ưu - 154 mg/l (độ nhạy 70,1%, độ đặc hiệu 55,6%), khoảng tin cậy 95% 0,698-0,819. Đường cong ROC cho ngày hậu phẫu thứ ba - AUC 0,715; giá trị CRP tối ưu - 144,5 mg/l (độ nhạy 79,1% độ đặc hiệu 60,3%), khoảng tin cậy 95% 0,68-0,75. Đường cong ROC cho ngày hậu phẫu thứ tư - AUC 0,767; giá trị CRP tối ưu - 122,91 mg/l (độ nhạy 72,3% độ đặc hiệu 60%), khoảng tin cậy 95% 0,73-0,804. KẾT LUẬN CRP tăng là yếu tố dự báo sớm AL sau phẫu thuật ung thư đại trực tràng. Mức CRP ≥144,5 mg/l vào ngày hậu phẫu thứ ba có thể dự đoán AL (độ nhạy 79%, độ đặc hiệu 60%)."} +{"idx": 1870089, "en": "The authors describe modern 3D technologies in hepatobiliary surgery. These approaches reduce the risk of intra- and postoperative complications. Virtual 3D reconstruction with clear visualization of parasitic cyst, adjacent vessels and bile ducts is valuable to create 3D-model of liver. This model may be applied for personalized laparoscopic approach and precise surgical intervention.", "gemini": "Các tác giả mô tả các công nghệ 3D hiện đại trong phẫu thuật gan mật. Những phương pháp tiếp cận này làm giảm nguy cơ biến chứng trong và sau phẫu thuật. Tái tạo 3D ảo với hình ảnh rõ nét về nang ký sinh, mạch máu và ống mật kề cận có giá trị để tạo mô hình 3D của gan. Mô hình này có thể được áp dụng cho phương pháp tiếp cận nội soi được cá nhân hóa và can thiệp phẫu thuật chính xác."} +{"idx": 1870090, "en": "Aneurysmal transformation of arteriovenous fistula (AVF) is a common complication and associated with increased risk of arrosive bleeding. Ligation of fistulous vein is conventional surgery for bleeding. This is followed by AVF malfunction and need for implantation of central venous catheter. We report reconstructive surgery and maintaining the function of AVF in a patient with aneurysmal transformation of AVF after arrosive bleeding. Reconstructive vascular surgery can significantly extend the period of patent AVF for hemodialysis. This is extremely important in patients with reduced resources of native vessels suitable for AVF formation. The same is true if conversion of the type of renal replacement therapy is associated with significant difficulties.", "gemini": "Sự biến đổi thành phình động mạch của rò động tĩnh mạch (AVF) là một biến chứng phổ biến và có liên quan đến nguy cơ chảy máu xói mòn tăng lên. Thắt tĩnh mạch rò là phẫu thuật thông thường để cầm máu. Tiếp theo là trục trặc AVF và cần phải cấy ghép ống thông tĩnh mạch trung tâm. Chúng tôi báo cáo phẫu thuật tái tạo và duy trì chức năng của AVF ở một bệnh nhân bị biến đổi thành phình động mạch của AVF sau khi chảy máu xói mòn. Phẫu thuật mạch máu tái tạo có thể kéo dài đáng kể thời gian AVF thông suốt để chạy thận nhân tạo. Điều này cực kỳ quan trọng ở những bệnh nhân có nguồn mạch máu tự nhiên phù hợp để tạo AVF bị giảm. Điều tương tự cũng đúng nếu việc chuyển đổi loại liệu pháp thay thế thận có liên quan đến những khó khăn đáng kể."} +{"idx": 1870091, "en": "The authors reported a rare clinical case of successful surgical treatment of young female with retroperitoneal leiomyosarcoma followed by lesion of the cavarenal segment of inferior vena cava, left renal vein. Clinical and morphological features of disease, postoperative outcomes and prognostic factors in patients with retroperitoneal leiomyosarcoma are discussed.", "gemini": "Các tác giả đã báo cáo một trường hợp lâm sàng hiếm gặp về việc điều trị phẫu thuật thành công cho một phụ nữ trẻ bị u leiomyosarcoma ở sau phúc mạc sau đó là tổn thương đoạn tĩnh mạch chủ dưới của tĩnh mạch chủ dưới, tĩnh mạch thận trái. Các đặc điểm lâm sàng và hình thái của bệnh, kết quả sau phẫu thuật và các yếu tố tiên lượng ở những bệnh nhân bị u leiomyosarcoma ở sau phúc mạc được thảo luận."} +{"idx": 1870092, "en": "Color changing food spoilage sensors for protein-based food products, such as fish and beef, are mostly based on the halochromic behavior of pH indicators. However, due to their reversible halochromic nature, these sensors can be manipulated by chemical treatment, hiding the true history and quality of deteriorated meat. Therefore, there is a need to create an irreversible and reliable food spoilage sensor, which clearly indicates to consumers if any food degradation or improper storage has occurred, and avoid nefarious food processing companies from disguising spoiled meat as fresh meat. Here, a simple, irreversible, and halochromic sensor showing spoilage of seafood and meat products is developed. Specifically, chlorophenol red (CPR)-fatty acid particles are dispersed within an ammonia-permeable polymer matrix to form a nontoxic film sensor that shows obvious halochromic behavior toward bioamine or total volatile basic nitrogen (TVB-N) given off by deteriorated seafood or meat products. After the removal of TVB-N, this sensor does not revert back to its original color due to a loss of π-π stacking of the original sulfonephthalein molecules. These features make this sensor applicable as a novel and reliable spoilage sensor for protein-based food products.", "gemini": "Các cảm biến hư hỏng thực phẩm đổi màu dành cho các sản phẩm thực phẩm có nguồn gốc từ protein, chẳng hạn như cá và thịt bò, chủ yếu dựa trên đặc tính halochromic của các chỉ thị pH. Tuy nhiên, do bản chất halochromic có thể đảo ngược của chúng, những cảm biến này có thể bị thao túng bằng xử lý hóa học, che giấu lịch sử và chất lượng thực sự của thịt bị hư hỏng. Do đó, cần tạo ra một cảm biến hư hỏng thực phẩm không thể đảo ngược và đáng tin cậy, chỉ rõ cho người tiêu dùng nếu bất kỳ sự xuống cấp thực phẩm nào hoặc bảo quản không đúng cách đã xảy ra và tránh các công ty chế biến thực phẩm bất chính ngụy trang thịt ôi thiu thành thịt tươi. Tại đây, một cảm biến đơn giản, không thể đảo ngược và halochromic cho thấy sự hư hỏng của hải sản và các sản phẩm từ thịt được phát triển. Cụ thể, các hạt axit béo chlorophenol đỏ (CPR) được phân tán trong một ma trận polyme thấm amoniac để tạo thành một cảm biến màng không độc hại có biểu hiện halochromic rõ ràng đối với bioamine hoặc tổng lượng nitơ bazơ dễ bay hơi (TVB-N) do hải sản hoặc thịt hư hỏng thải ra. sản phẩm. Sau khi loại bỏ TVB-N, cảm biến này không trở lại màu ban đầu do mất sự xếp chồng π-π của các phân tử sulfonephthalein ban đầu. Những đặc điểm này làm cho cảm biến này có thể áp dụng như một cảm biến hư hỏng mới và đáng tin cậy cho các sản phẩm thực phẩm có nguồn gốc từ protein."} +{"idx": 1870093, "en": "A series of trans-dicyanide vanadium(III) compounds based on acetylacetonate, (PPN)[VIII(acac)2(CN)2]·(PPN)Cl·2MeCN (1), and salen ligands, (Et4N)[VIII(salen)(CN)2] (2a), (PPN)[VIII(MeOsalen)(CN)2]·DMF·2MeCN (3), and (PPN)[VIII(salphen)(CN)2]·DMF (4) [salen = N,N'-ethylenebis(salicyl-imine), MeOsalen = N,N'-ethylenebis(methoxysalicylimine), salphen = N,N'-phenylenebis(salicyl-imine), and PPN = bis(triphenylphosphine)iminium], were prepared and structurally characterized. High-field EPR studies reveal that the complexes exhibit moderate magnetic anisotropy with positive D values of +5.70, +3.80, +4.05, and +3.99 cm-1 for 1-4, respectively.", "gemini": "Một loạt các hợp chất vanadi(III) xuyên dicyanua dựa trên acetylacetonate, (PPN)[VIII(acac)2(CN)2]·(PPN)Cl·2MeCN (1) và phối tử salen, (Et4N)[VIII(salen)(CN)2] (2a), (PPN)[VIII(MeOsalen)(CN)2]·DMF·2MeCN (3) và (PPN)[VIII(salphen)(CN)2]·DMF (4) [salen = N,N'-etylenebis(salicyl-imine), MeOsalen = N,N'-etylenebis(methoxysalicylimine), salphen = N,N'-phenylenebis(salicyl-imine) và PPN = bis(triphenylphosphine)iminium], đã được điều chế và đặc trưng về mặt cấu trúc. Các nghiên cứu EPR trường cao cho thấy rằng các phức chất biểu hiện tính dị hướng từ trung bình với các giá trị D dương là +5,70, +3,80, +4,05 và +3,99 cm-1 cho 1-4, tương ứng."} +{"idx": 1870094, "en": "Bi and Pb ions with charge degree of freedom depending on 6s2 and 6s0 electronic configurations were combined with the Mn ion in a perovskite oxide. Comprehensive theoretical and experimental investigations revealed the Bi3+0.5Pb2+0.5Mn3+0.5Mn4+0.5O3 charge ordered state with CE-type spin and dz2 orbital orderings as observed in La0.5Ca0.5MnO3, Nd0.5Sr0.5MnO3, and Bi0.5Sr0.5MnO3. The charge and orbital orderings were preserved above 500 K owing to the stereochemical activity of Bi3+ and Pb2+ ions which stabilized the structural distortion.", "gemini": "Các ion Bi và Pb với mức độ tự do điện tích phụ thuộc vào cấu hình điện tử 6s2 và 6s0 đã được kết hợp với ion Mn trong một oxit perovskite. Các nghiên cứu lý thuyết và thực nghiệm toàn diện đã chỉ ra trạng thái sắp xếp điện tích Bi3+0,5Pb2+0,5Mn3+0,5Mn4+0,5O3 với kiểu sắp xếp spin CE và sắp xếp quỹ đạo dz2 như quan sát thấy trong La0,5Ca0,5MnO3, Nd0,5Sr0,5MnO3 và Bi0,5Sr0,5MnO3. Các sắp xếp điện tích và quỹ đạo được bảo toàn trên 500 K do hoạt động lập thể của các ion Bi3+ và Pb2+ giúp ổn định sự biến dạng cấu trúc."} +{"idx": 1870095, "en": "Digital nucleic acid amplification testing (dNAAT) and analysis techniques, such as digital polymerase chain reaction (PCR), have become useful clinical diagnostic tools. However, nucleic acid (NA) sample preparation preceding dNAAT is generally laborious and performed manually, thus creating the need for a simple sample preparation technique and a facile coupling strategy for dNAAT. Therefore, we demonstrate a simple workflow which automates magnetic bead-based extraction of NAs with a one-step transfer to dNAAT. Specifically, we leverage droplet magnetofluidics (DM) to automate the movement of magnetic beads between small volumes of reagents commonly employed for NA extraction and purification. Importantly, the buffer typically used to elute the NAs off the magnetic beads is replaced by a carefully selected PCR solution, enabling direct transfer from sample preparation to dNAAT. Moreover, we demonstrate the potential for multiplexing using a digital high-resolution melt (dHRM) after the digital PCR (dPCR). The utility of this workflow is demonstrated with duplexed detection of bacteria in a sample imitating a coinfection. We first purify the bacterial DNA into a PCR solution using our DM-based sample preparation. We then transfer the purified bacterial DNA to our microfluidic nanoarray to amplify 16S rRNA using dPCR and then perform dHRM to identify the two bacterial species.", "gemini": "Kỹ thuật phân tích và thử nghiệm khuếch đại axit nucleic kỹ thuật số (dNAAT), chẳng hạn như phản ứng chuỗi polymerase kỹ thuật số (PCR), đã trở thành các công cụ chẩn đoán lâm sàng hữu ích. Tuy nhiên, việc chuẩn bị mẫu axit nucleic (NA) trước dNAAT thường rất tốn công và được thực hiện thủ công, do đó tạo ra nhu cầu về một kỹ thuật chuẩn bị mẫu đơn giản và một chiến lược ghép nối dễ dàng cho dNAAT. Do đó, chúng tôi trình bày một quy trình làm việc đơn giản để tự động hóa việc chiết xuất NA dựa trên hạt từ tính với một bước chuyển sang dNAAT. Cụ thể, chúng tôi tận dụng từ lưu biến giọt (DM) để tự động hóa chuyển động của các hạt từ tính giữa các thể tích nhỏ thuốc thử thường được sử dụng để chiết xuất và tinh chế NA. Quan trọng hơn, dung dịch đệm thường được sử dụng để rửa trôi các NA khỏi các hạt từ tính được thay thế bằng dung dịch PCR được lựa chọn cẩn thận, cho phép chuyển trực tiếp từ khâu chuẩn bị mẫu sang dNAAT. Hơn nữa, chúng tôi chứng minh tiềm năng ghép kênh bằng cách sử dụng độ tan chảy phân giải cao kỹ thuật số (dHRM) sau phản ứng PCR kỹ thuật số (dPCR). Tính hữu ích của quy trình làm việc này được chứng minh bằng việc phát hiện song song vi khuẩn trong một mẫu mô phỏng tình trạng nhiễm trùng đồng thời. Đầu tiên, chúng tôi tinh chế DNA vi khuẩn thành dung dịch PCR bằng cách sử dụng quy trình chuẩn bị mẫu dựa trên DM của chúng tôi. Sau đó, chúng tôi chuyển DNA vi khuẩn đã tinh chế vào mảng nano vi lưu của mình để khuếch đại rRNA 16S bằng dPCR và sau đó thực hiện dHRM để xác định hai loài vi khuẩn."} +{"idx": 1870096, "en": "Owing to the development of microelectronics, demands for excellent thermal dissipation materials have substantially increased. Learning from natural nacre, thermally conductive epoxy nanocomposites were prepared based on asymmetrically polydopamine-functionalized Janus graphene oxide (JPGO) scaffolds. The required highly oriented JPGO scaffolds were prepared via the bidirectional freeze-casting method. With the addition of epoxy resin, the resulting nanocomposite reveals anisotropic thermal properties. With the total content of the JPGO scaffold being 0.93 wt %, almost 35 times enhancement of in-plane thermal conductivity (perpendicular to the lamellar structure) (∼5.6 W m-1 K-1) has been obtained. The single-side-functionalized JPGO scaffolds play an important role in forming thermal conductive networks for the epoxy nanocomposites. Importantly, the nanocomposites present electrically insulating properties (>1014 Ω cm). Such high-performance nanocomposites have promising applications for thermal management in electronic devices.", "gemini": "Do sự phát triển của vi điện tử, nhu cầu về vật liệu tản nhiệt tuyệt vời đã tăng lên đáng kể. Học hỏi từ xà cừ tự nhiên, vật liệu nanocomposite epoxy dẫn nhiệt đã được chế tạo dựa trên khung giàn graphene oxit Janus (JPGO) được chức năng hóa polydopamine không đối xứng. Các khung giàn JPGO có hướng cao cần thiết đã được chế tạo thông qua phương pháp đúc đông hướng hai chiều. Với việc bổ sung nhựa epoxy, vật liệu nanocomposite thu được cho thấy các tính chất nhiệt dị hướng. Với tổng hàm lượng khung giàn JPGO là 0,93 wt%, đã thu được sự tăng cường gần 35 lần độ dẫn nhiệt trong mặt phẳng (vuông góc với cấu trúc dạng phiến) (∼5,6 W m-1 K-1). Các khung giàn JPGO được chức năng hóa một mặt đóng vai trò quan trọng trong việc hình thành các mạng dẫn nhiệt cho vật liệu nanocomposite epoxy. Điều quan trọng là vật liệu nanocomposite thể hiện các tính chất cách điện (>1014 Ω cm). Những vật liệu nanocomposite hiệu suất cao như vậy có ứng dụng đầy hứa hẹn cho việc quản lý nhiệt trong các thiết bị điện tử."} +{"idx": 1870097, "en": "Luteolin, a flavonoid widely distributed in the plant kingdom, contains two benzene rings and hydroxyl groups, and this structural specificity contributes to its diverse biological activities. However, no previous studies have simultaneously investigated the therapeutic potency of luteolin isolated from a plant as an antipsychotic and antidepressant. Here, luteolin exhibited selective inhibition of hMAO-A (IC50 = 8.57 ± 0.47 μM) over hMAO-B (IC50 > 100 μM). In silico proteochemometric modeling predicted promising targets of luteolin, and verification via cell-based G protein-coupled receptor functional assays showed that luteolin is a selective antagonist of the vasopressin receptor V1AR (IC50 = 19.49 ± 6.32 μM) and the dopamine D4 receptor (IC50 = 39.59 ± 1.46 μM). Molecular docking showed the tight binding of luteolin with a low binding score and the high stability of the luteolin-receptor complex, corroborating its functional effect. Thus, hMAO-A, hD4R, and hV1AR are prime targets of luteolin and potential alternatives for the management of neurodegenerative diseases.", "gemini": "Luteolin, một flavonoid phân bố rộng rãi trong thực vật, chứa hai vòng benzen và nhóm hydroxyl, và cấu trúc đặc hiệu này góp phần tạo nên các hoạt tính sinh học đa dạng của nó. Tuy nhiên, chưa có nghiên cứu nào trước đây đồng thời điều tra hiệu lực điều trị của luteolin được phân lập từ thực vật như một chất chống loạn thần và chống trầm cảm. Ở đây, luteolin cho thấy khả năng ức chế chọn lọc hMAO-A (IC50 = 8,57 ± 0,47 μM) so với hMAO-B (IC50> 100 μM). Mô hình hóa proteochemometric in silico dự đoán các mục tiêu đầy hứa hẹn của luteolin và xác minh thông qua xét nghiệm chức năng thụ thể gắn protein G dựa trên tế bào cho thấy luteolin là chất đối kháng chọn lọc của thụ thể vasopressin V1AR (IC50 = 19,49 ± 6,32 μM) và thụ thể dopamine D4 (IC50 = 39,59 ± 1,46 μM). Đối ghép phân tử cho thấy sự liên kết chặt chẽ của luteolin với điểm liên kết thấp và độ ổn định cao của phức hợp luteolin-thụ thể, chứng minh tác dụng chức năng của nó. Do đó, hMAO-A, hD4R và hV1AR là các mục tiêu chính của luteolin và là các lựa chọn thay thế tiềm năng cho việc quản lý các bệnh thoái hóa thần kinh."} +{"idx": 1870098, "en": "Design of highly active carbon nanozymes and further establishment of ultrasensitive biosensors remain a challenge. Herein, hierarchically porous carbon nanozymes with sulfur (S)/nitrogen (N) codoping (SNC) were developed. Compared with N-doped carbon (NC) nanozymes, SNC nanozymes have a smaller Michaelis-Menten constant and higher specific activities, demonstrating that the S-doping in SNC nanozymes could not only enhance their affinity toward substrates but also improve their catalytic performance. These results may be caused by the synergistic effect of heteroatoms (S and N). Because of the good enzyme-like activity, the proposed SNC nanozymes were exploited to the colorimetric detection of the total antioxidant capacity (TAC) using ascorbic acid as a typical model with a limit of detection of 0.08 mM. Because of its high sensitivity and selectivity and encouraging performance, the detection method presented practical feasibility for the TAC assay in commercial beverages. This work paves a way to design the highly active carbon nanozymes and expand their applications in the construction of high-performance biosensors.", "gemini": "Thiết kế các nanozyme carbon hoạt tính cao và thiết lập thêm các cảm biến sinh học siêu nhạy vẫn là một thách thức. Tại đây, các nanozyme carbon xốp phân cấp với lưu huỳnh (S)/nitơ (N) đồng bổ sung (SNC) đã được phát triển. So với nanozyme carbon (NC) được bổ sung N, nanozyme SNC có hằng số Michaelis-Menten nhỏ hơn và hoạt tính đặc hiệu cao hơn, chứng tỏ rằng việc bổ sung S vào nanozyme SNC không chỉ có thể tăng cường ái lực của chúng đối với chất nền mà còn cải thiện hiệu suất xúc tác của chúng. Những kết quả này có thể là do hiệu ứng hiệp đồng của các nguyên tử tạp (S và N). Do hoạt động giống enzyme tốt, các nanozyme SNC được đề xuất đã được khai thác để phát hiện tổng dung lượng chống oxy hóa (TAC) bằng phương pháp đo màu sử dụng axit ascorbic làm mô hình điển hình với giới hạn phát hiện là 0,08 mM. Do độ nhạy và độ chọn lọc cao cùng hiệu suất đáng khích lệ, phương pháp phát hiện này thể hiện tính khả thi thực tế đối với xét nghiệm TAC trong đồ uống thương mại. Công trình này mở ra một con đường để thiết kế các nanozyme carbon hoạt tính cao và mở rộng ứng dụng của chúng trong việc chế tạo các cảm biến sinh học hiệu suất cao."} +{"idx": 1870099, "en": "There is a need for photocatalysts with efficient photocarrier separation to address issues with environmental pollution. Photocarrier separation is largely determined by the orbital composition near the band edge. Here, we investigate Zn4B6O13 as an efficient photocatalyst for photodegradation of tetracycline. Theoretical calculations of Zn4B6O13 show that the valence band near the Fermi level is composed of d and p orbitals whereas the bottom of the conduction band is composed of s and p orbitals; thus, a large value of mh/me is derived from the band dispersion. The characteristics of this orbital composition promote separation of photoexcited carriers, leading to a high transfer efficiency of the catalyst. Moreover, photodegradation experiments demonstrate that the photocatalytic activity of Zn4B6O13 is approximately 5.2 times as high as that of SnO2. This study provides insights that might aid the development of novel borate-based environmental photocatalysts with superior performance.", "gemini": "Cần có các chất quang xúc tác với khả năng tách quang điện tích hiệu quả để giải quyết các vấn đề về ô nhiễm môi trường. Sự tách quang điện tích phần lớn được xác định bởi thành phần quỹ đạo gần mép dải. Ở đây, chúng tôi nghiên cứu Zn4B6O13 như một chất quang xúc tác hiệu quả cho quá trình quang phân hủy tetracycline. Các tính toán lý thuyết về Zn4B6O13 cho thấy dải hóa trị gần mức Fermi bao gồm các quỹ đạo d và p trong khi đáy của dải dẫn bao gồm các quỹ đạo s và p; do đó, một giá trị lớn của mh/me có nguồn gốc từ sự phân tán của dải. Các đặc tính của thành phần quỹ đạo này thúc đẩy sự tách các hạt mang điện kích thích bằng ánh sáng, dẫn đến hiệu suất truyền cao của chất xúc tác. Hơn nữa, các thí nghiệm quang phân hủy chứng minh rằng hoạt tính quang xúc tác của Zn4B6O13 cao hơn khoảng 5,2 lần so với SnO2. Nghiên cứu này cung cấp những hiểu biết có thể hỗ trợ phát triển các chất quang xúc tác môi trường mới dựa trên borat có hiệu suất vượt trội."} +{"idx": 1870100, "en": "During the past few decades, fabrication of functional rotaxane-branched dendrimers has become one of the most attractive yet challenging topics within supramolecular chemistry and materials science. Herein, we present the successful fabrication of a family of new rotaxane-branched dendrimers containing up to 21 platinum atoms and 42 photosensitizer moieties through an efficient and controllable divergent approach. Notably, the photosensitization efficiencies of these rotaxane-branched dendrimers gradually increased with the increase of dendrimer generation. For example, third-generation rotaxane-branched dendrimer PG3 revealed 13.3-fold higher 1O2 generation efficiency than its corresponding monomer AN. The enhanced 1O2 generation efficiency was attributed to the enhancement of intersystem crossing (ISC) through the simple and efficient incorporation of multiple heavy atoms and photosensitizer moieties on the axles and wheels of the rotaxane units, respectively, which has been validated by UV-visible and fluorescence techniques, time-dependent density functional theory calculations, photolysis model reactions, and apparent activation energy calculations. Therefore, we develop a new promising platform of rotaxane-branched dendrimers for the preparation of effective photosensitizers.", "gemini": "Trong vài thập kỷ qua, việc chế tạo các dendrimer phân nhánh rotaxane chức năng đã trở thành một trong những chủ đề hấp dẫn nhưng đầy thách thức nhất trong lĩnh vực hóa học siêu phân tử và khoa học vật liệu. Trong bài báo này, chúng tôi trình bày thành công việc chế tạo một họ các dendrimer phân nhánh rotaxane mới chứa tới 21 nguyên tử bạch kim và 42 nhóm quang nhạy thông qua một phương pháp phân kỳ hiệu quả và có thể kiểm soát được. Đáng chú ý, hiệu suất quang nhạy của các dendrimer phân nhánh rotaxane này tăng dần theo sự gia tăng của thế hệ dendrimer. Ví dụ, dendrimer phân nhánh rotaxane thế hệ thứ ba PG3 cho thấy hiệu suất tạo 1O2 cao hơn 13,3 lần so với monome tương ứng AN. Hiệu suất tạo 1O2 tăng cường được quy cho sự tăng cường giao thoa hệ thống liên hợp (ISC) thông qua sự kết hợp đơn giản và hiệu quả của nhiều nguyên tử nặng và nhóm quang nhạy trên trục và bánh xe của các đơn vị rotaxane, tương ứng, đã được xác nhận bằng các kỹ thuật UV-VIS và huỳnh quang, tính toán lý thuyết hàm mật độ phụ thuộc thời gian, phản ứng mô hình quang phân và tính toán năng lượng hoạt hóa biểu kiến. Do đó, chúng tôi phát triển một nền tảng mới đầy hứa hẹn của các dendrimer phân nhánh rotaxane để chế tạo các chất quang nhạy hiệu quả."} +{"idx": 1870101, "en": "Designing strategies to utilize the synergistic effect of probiotics and prebiotics is a promising way in treating metabolic-related diseases. Here, inspired by the mutually promotable but mutually incompatible characteristics of Yin and Yang, dual-core microcapsules that encapsulate Lactobacillus and Bacillus subtilis into separate compartments were presented through electrostatically driven microfluidics. The microcapsules showed acid resistance and preserved probiotic activity. They also fostered the proliferation of probiotics while creating an anaerobic environment and promoted lactic acid fermentation without affecting each other. It has been demonstrated that the microcapsules could reduce inflammation, improve fat metabolism, and restore intestinal barrier functions, thus contributing to the treatment of metabolic syndrome in vivo. These features make the dual-core microcapsules an ideal candidate for treating metabolic syndrome and related diseases.", "gemini": "Thiết kế các chiến lược để sử dụng hiệu ứng hiệp đồng của vi khuẩn probiotic và prebiotic là một cách đầy hứa hẹn trong điều trị các bệnh liên quan đến chuyển hóa. Ở đây, lấy cảm hứng từ các đặc điểm tương hỗ nhưng không tương thích của Âm và Dương, các vi nang kép lõi bao bọc Lactobacillus và Bacillus subtilis vào các ngăn riêng biệt đã được trình bày thông qua kỹ thuật vi lưu thể tĩnh điện. Các vi nang cho thấy khả năng kháng axit và bảo toàn hoạt tính của vi khuẩn probiotic. Chúng cũng thúc đẩy sự phát triển của vi khuẩn probiotic trong khi tạo ra môi trường kỵ khí và thúc đẩy quá trình lên men axit lactic mà không ảnh hưởng đến nhau. Người ta đã chứng minh rằng các vi nang có thể giảm viêm, cải thiện quá trình chuyển hóa chất béo và phục hồi chức năng hàng rào ruột, do đó góp phần vào việc điều trị hội chứng chuyển hóa in vivo. Những đặc điểm này làm cho các vi nang kép lõi trở thành ứng cử viên lý tưởng để điều trị hội chứng chuyển hóa và các bệnh liên quan."} +{"idx": 1870102, "en": "In the present paper, new functionalized UiO-67 metal-organic frameworks (MOF) which contain aromatic tagged groups such as phenyl, naphthyl, and anthracene have been synthesized, characterized, and used for sensing water-soluble amino acids. The results show that anthracene-tagged UiO-67-MOF is shown to act as a highly efficient and selective aqueous sensor for arginine over other water-soluble amino acids in nanoscale. Upon adding an increasing amount of arginine, PL bands of the anth-UiO-67 MOF quenched completely, while there is no perturbation in the PL bands for other amino acid observed. This MOF allows a selective ratiometric detection of arginine without any interference from other amino acids.", "gemini": "Trong bài báo này, các khung kim loại hữu cơ UiO-67 chức năng hóa mới (MOF) có chứa các nhóm gắn thơm như phenyl, naphthyl và anthracen đã được tổng hợp, đặc trưng và sử dụng để cảm biến các axit amin tan trong nước. Kết quả cho thấy UiO-67-MOF gắn anthracen hoạt động như một cảm biến nước hiệu quả cao và chọn lọc đối với arginine so với các axit amin tan trong nước khác ở thang nano. Khi thêm một lượng arginine ngày càng tăng, các dải PL của MOF anth-UiO-67 bị dập tắt hoàn toàn, trong khi không có nhiễu loạn nào trong các dải PL đối với các axit amin khác được quan sát thấy. MOF này cho phép phát hiện tỷ lệ chọn lọc arginine mà không có bất kỳ sự can thiệp nào từ các axit amin khác."} +{"idx": 1870103, "en": "In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characterized after UPLC-MS/MS, was screened out by macrophage inflammatory cytokine production assay. RNA-sequencing analysis was performed to explore the mechanisms underlying the anti-inflammatory activity associated with this peptide. The results indicated that lupin peptides effectively inhibited the lipopolysaccharide-induced overproduction of proinflammatory mediators. IQD inhibited the production of tumor necrosis factor-α, interleukin (IL)-6, IL-1β, and monocyte chemoattractant protein-1 by 51.20, 38.52, 44.70, and 40.43%, respectively. RNA-sequencing results showed that IQD inhibited the inflammatory response by regulating the gene expression of the p38 mitogen-activated protein kinase pathway and inhibiting downstream inflammatory cytokines. These bioactive peptides may be used to develop new ingredients for anti-inflammatory nutritional supplements.", "gemini": "Trong nghiên cứu này, protein được chiết xuất từ hạt lupin ép đùn và đưa vào dịch tiêu hóa dạ dày-tá tràng để thu được các peptide lupin, được đặc trưng bằng sắc ký lỏng hiệu năng cực cao ghép nối với phổ khối (UPLC-MS/MS). Sau đó, IQDKEGIPPDQQR (IQD), monome peptide lupin được đặc trưng sau UPLC-MS/MS, được sàng lọc bằng xét nghiệm sản xuất cytokine viêm của đại thực bào. Phân tích giải trình tự RNA đã được thực hiện để khám phá các cơ chế cơ bản của hoạt động chống viêm liên quan đến peptide này. Kết quả cho thấy các peptide lupin ức chế hiệu quả sự sản xuất quá mức các chất trung gian gây viêm do lipopolysaccharide gây ra. IQD ức chế sự sản xuất của yếu tố hoại tử khối u-α, interleukin (IL)-6, IL-1β và protein hấp dẫn chemoattractant đơn bào-1 lần lượt là 51,20, 38,52, 44,70 và 40,43%. Kết quả giải trình tự RNA cho thấy IQD ức chế phản ứng viêm bằng cách điều chỉnh biểu hiện gen của con đường protein kinase hoạt hóa mitogen p38 và ức chế các cytokine viêm hạ nguồn. Các peptide hoạt tính sinh học này có thể được sử dụng để phát triển các thành phần mới cho các chất bổ sung dinh dưỡng chống viêm."} +{"idx": 1870104, "en": "Lanthanide-based luminescent nanothermometers play a crucial role in optical temperature determination. However, because of the strong thermal quenching of the luminescence, as well as the deterioration of their sensitivity and resolution with temperature elevation, they can operate in a relatively low-temperature range, usually from cryogenic to ≈800 K. In this work, we show how to overcome these limitations and monitor very high-temperature values, with high sensitivity (≈2.1% K-1) and good thermal resolution (≈1.4 K) at around 1000 K. As an optical probe of temperature, we chose upconverting Yb3+-Tm3+ codoped YVO4 nanoparticles. For ratiometric sensing in the low-temperature range, we used the relative intensities of the Tm3+ emissions associated with the 3F2,3 and 3H4 thermally coupled levels, that is, 3F2,3 → 3H6/3H4 → 3H6 (700/800 nm) band intensity ratio. In order to improve sensitivity and resolution in the high-temperature range, we used the 940/800 nm band intensity ratio of the nonthermally coupled levels of Yb3+ (2F5/2 → 2F7/2) and Tm3+ (3H4 → 3H6). These NIR bands are very intense, even at extreme temperature values, and their intensity ratio changes significantly, allowing accurate temperature sensing with high thermal and spatial resolutions. The results presented in this work may be particularly important for industrial applications, such as metallurgy, catalysis, high-temperature synthesis, materials processing and engineering, and so forth, which require rapid, contactless temperature monitoring at extreme conditions.", "gemini": "Nhiệt kế nano phát quang dựa trên lanthanide đóng vai trò rất quan trọng trong việc xác định nhiệt độ quang học. Tuy nhiên, do sự dập tắt nhiệt mạnh của phát quang, cũng như độ nhạy và độ phân giải của chúng bị suy giảm khi nhiệt độ tăng, chúng có thể hoạt động trong phạm vi nhiệt độ tương đối thấp, thường từ nhiệt độ lạnh đến ≈800 K. Trong nghiên cứu này, chúng tôi chỉ ra cách khắc phục những hạn chế này và theo dõi các giá trị nhiệt độ rất cao, với độ nhạy cao (≈2,1% K-1) và độ phân giải nhiệt tốt (≈1,4 K) ở khoảng 1000 K. Là đầu dò nhiệt độ quang học, chúng tôi chọn các hạt nano YVO4 đồng phủ Yb3+-Tm3+ chuyển đổi hướng lên. Đối với cảm biến tỷ lệ trong phạm vi nhiệt độ thấp, chúng tôi sử dụng cường độ tương đối của các phát xạ Tm3+ liên quan đến các mức ghép nhiệt 3F2,3 và 3H4, tức là tỷ lệ cường độ dải 3F2,3 → 3H6/3H4 → 3H6 (700/800 nm). Để cải thiện độ nhạy và độ phân giải trong phạm vi nhiệt độ cao, chúng tôi sử dụng tỷ lệ cường độ dải 940/800 nm của các mức không ghép nhiệt của Yb3+ (2F5/2 → 2F7/2) và Tm3+ (3H4 → 3H6). Các dải NIR này rất mạnh, ngay cả ở các giá trị nhiệt độ cực đại và tỷ lệ cường độ của chúng thay đổi đáng kể, cho phép cảm biến nhiệt độ chính xác với độ phân giải nhiệt và không gian cao. Các kết quả trình bày trong nghiên cứu này có thể đặc biệt quan trọng đối với các ứng dụng công nghiệp, chẳng hạn như luyện kim, xúc tác, tổng hợp nhiệt độ cao, chế biến và kỹ thuật vật liệu, v.v., đòi hỏi phải theo dõi nhiệt độ nhanh chóng, không tiếp xúc trong điều kiện khắc nghiệt."} +{"idx": 1870105, "en": "Tunable diode laser absorption spectroscopy (TDLAS) based on a multipass cell (MPC) is a powerful analytical tool and is widely applied to air quality monitoring, industrial process control, and medical diagnostics. However, the conventional MPC as a core component in TDLAS devices has a large size, low utilization efficiency of the mirror surfaces, and tight optical alignment tolerances. In this paper, we design and fabricate a mini-MPC with an optical absorption path length of 4.2 m and dimensions of 4 × 4 × 6 cm3 (open cavity), which, to our best knowledge, is the current smallest MPC in terms of the same optical path length. The mini-MPC generates a seven-nonintersecting-circle dense spot pattern on two 25.4 mm spherical mirror surfaces, providing a high fill factor of 21 cm-2. A fiber-coupled collimator and an InGaAs photodetector are integrated into the mini-MPC via a high-resolution three-dimensional printed frame, hence removing the requirement of active optical alignment. Using a 1.65 μm distributed-feedback laser, the performance of this mini-MPC for methane detection was evaluated in terms of linearity, flow response time, stability, minimum detectable limit, and measurement precision. Continuous measurements of methane near a sewer and in the atmosphere were performed to demonstrate the stability and robustness of the highly integrated mini-MPC-based gas sensor. Our analysis shows that a methane minimum detectable limit of 117 ppbv is achieved, paving the way toward a sensitive, low-cost, and miniature trace gas sensor inherently suitable for large-scale deployment of distributed sensor networks and for handheld mobile devices.", "gemini": "Phổ hấp thụ laser diode có thể điều chỉnh (TDLAS) dựa trên một ô đa chùm tia (MPC) là một công cụ phân tích mạnh mẽ và được ứng dụng rộng rãi để theo dõi chất lượng không khí, kiểm soát quy trình công nghiệp và chẩn đoán y tế. Tuy nhiên, MPC thông thường là một thành phần cốt lõi trong các thiết bị TDLAS có kích thước lớn, hiệu suất sử dụng thấp các bề mặt gương và dung sai căn chỉnh quang học chặt chẽ. Trong bài báo này, chúng tôi thiết kế và chế tạo một MPC mini có độ dài đường dẫn hấp thụ quang là 4,2 m và kích thước 4 × 4 × 6 cm3 (khoang hở), theo hiểu biết tốt nhất của chúng tôi, đây là MPC nhỏ nhất hiện tại về cùng độ dài đường dẫn quang. MPC mini tạo ra một mẫu điểm dày đặc bảy vòng tròn không giao nhau trên hai bề mặt gương cầu 25,4 mm, cung cấp hệ số lấp đầy cao là 21 cm-2. Bộ chỉnh độ hội tụ được ghép sợi quang và bộ dò quang InGaAs được tích hợp vào MPC mini thông qua khung in ba chiều có độ phân giải cao, do đó loại bỏ yêu cầu căn chỉnh quang học chủ động. Sử dụng laser phản hồi phân tán 1,65 μm, hiệu suất của MPC mini này đối với việc phát hiện metan đã được đánh giá về độ tuyến tính, thời gian phản hồi dòng chảy, độ ổn định, giới hạn phát hiện tối thiểu và độ chính xác đo. Các phép đo liên tục metan gần cống rãnh và trong khí quyển đã được thực hiện để chứng minh độ ổn định và độ chắc chắn của cảm biến khí dựa trên MPC mini tích hợp cao. Phân tích của chúng tôi cho thấy đạt được giới hạn phát hiện metan tối thiểu là 117 ppbv, mở đường cho cảm biến khí vết nhạy, giá thành thấp và thu nhỏ vốn phù hợp cho việc triển khai mạng cảm biến phân tán quy mô lớn và cho các thiết bị di động cầm tay."} +{"idx": 1870106, "en": "A novel benchtop approach to fabricate xurography-enabled thermally transferred (XTT) carbon nanomaterial-based electrochemical sensors is proposed. Filtered nanomaterial (NM) films were transferred from Teflon filters to polyethylene terephthalate-ethylene vinyl acetate (PET-EVA) substrates by a temperature-driven approach. Customized PET-EVA components were xurographically patterned by a cutting plotter. The smart design of PET-EVA films enabled us to selectively transfer the nanomaterial to the exposed EVA side of the substrate. Hence, the substrate played an active role in selectively controlling where nanomaterial transfer occurred allowing us to design different working electrode geometries. Counter and reference electrodes were integrated by a stencil-printing approach, and the whole device was assembled by thermal lamination. To prove the versatility of the technology, XTT materials were exclusively made of carbon black (XTT-CB), multiwalled carbon nanotubes (XTT-MWCNTs), and single-walled carbon nanotubes (XTT-SWCNTs). Their electrochemical behavior was carefully studied and was found to be highly dependent on the amount and type of NM employed. XTT-SWCNTs were demonstrated to be the best-performing sensors, and they were employed for the determination of l-tyrosine (l-Tyr) in human plasma from tyrosinemia-diagnosed patients. High analytical performance toward l-Tyr (linear range of 0.5-100 μM, LOD = 0.1 μM), interelectrode precision (RSD ip,a = 3%, n = 10; RSD calibration slope = 4%, n = 3), and accurate l-Tyr quantification in plasma samples with low relative errors (≤7%) compared to the clinical declared values were obtained. The proposed benchtop approach is cost-effective and straightforward, does not require sophisticated facilities, and can be potentially employed to develop pure or hybrid nanomaterial-based electrodes.", "gemini": "Một phương pháp tiếp cận trên mặt bàn mới để chế tạo các cảm biến điện hóa dựa trên vật liệu nano carbon chuyển nhiệt được kích hoạt bằng xurography (XTT) được đề xuất. Các màng vật liệu nano (NM) đã lọc được chuyển từ bộ lọc Teflon sang chất nền polyethylene terephthalate-ethylene vinyl acetate (PET-EVA) bằng phương pháp dẫn nhiệt. Các thành phần PET-EVA tùy chỉnh được tạo mẫu xurographically bằng máy cắt vẽ. Thiết kế thông minh của màng PET-EVA cho phép chúng tôi chuyển vật liệu nano một cách chọn lọc sang mặt EVA tiếp xúc của chất nền. Do đó, chất nền đóng vai trò tích cực trong việc kiểm soát chọn lọc nơi xảy ra quá trình chuyển vật liệu nano, cho phép chúng tôi thiết kế các hình dạng điện cực làm việc khác nhau. Các điện cực đối và tham chiếu được tích hợp bằng phương pháp in lưới và toàn bộ thiết bị được lắp ráp bằng cách cán nhiệt. Để chứng minh tính linh hoạt của công nghệ, vật liệu XTT được làm riêng từ muội than (XTT-CB), ống nano carbon nhiều thành (XTT-MWCNT) và ống nano carbon thành đơn (XTT-SWCNT). Hành vi điện hóa của chúng đã được nghiên cứu cẩn thận và được phát hiện là phụ thuộc rất nhiều vào lượng và loại NM được sử dụng. XTT-SWCNT đã được chứng minh là cảm biến có hiệu suất tốt nhất và chúng được sử dụng để xác định l-tyrosine (l-Tyr) trong huyết tương người từ những bệnh nhân được chẩn đoán mắc bệnh tyrosin máu. Hiệu suất phân tích cao đối với l-Tyr (phạm vi tuyến tính 0,5-100 μM, LOD = 0,1 μM), độ chính xác giữa các điện cực (RSD ip, a = 3%, n = 10; độ dốc hiệu chuẩn RSD = 4%, n = 3) và định lượng l-Tyr chính xác trong các mẫu huyết tương với sai số tương đối thấp (≤7%) so với các giá trị lâm sàng đã công bố thu được. Phương pháp tiếp cận trên mặt bàn được đề xuất có hiệu quả về chi phí và đơn giản, không yêu cầu các cơ sở phức tạp và có khả năng được sử dụng để phát triển các điện cực dựa trên vật liệu nano tinh khiết hoặc lai."} +{"idx": 1870107, "en": "Metal-organic framework (MOF)-based electrolytes under gel/solid states have been widely used for electrochemical devices recently due to their designable metal centers/ligands and diffusion channels in the porous structures. Therefore, it is always desired to apply the MOF-based electrolytes in electrochromic (EC) fields. Yet, challenges exist in realizing their high optical transparency to satisfy the unique optical requirements of EC devices. Herein, a transparent MOF-based gel electrolyte (MGE) is demonstrated through the incorporation of 2-methylimidazole among MOF nanocrystals to prevent the strong light scattering of MOF nanocrystals. As a result, the gel electrolyte showed an improved average transmittance of ca. 82.2% compared with the MOF electrolytes without 2-methylimidazole (ca. 59.2%). In addition, because of the designed large channels in the porous MOF structure, the gel electrolyte exhibited a high ionic conductivity of 2.66 × 10-3 S cm-1. At last, we used the transparent MGEs to assemble two types (rigid and flexible) of quasi-solid-state EC devices based on inorganic WO3 and organic poly(3,4-ethylenedioxythiophene) (PEDOT), respectively. Both devices showed great EC performances, and the flexible devices exhibited high mechanical stability under the bending state or even after being cut and punched, advancing the general applications of our transparent MGEs in EC fields.", "gemini": "Các chất điện phân dựa trên khung kim loại-hữu cơ (MOF) ở trạng thái gel/rắn gần đây đã được sử dụng rộng rãi cho các thiết bị điện hóa do các kênh khuếch tán và các phối tử/trung tâm kim loại có thể thiết kế được trong các cấu trúc xốp. Do đó, người ta luôn mong muốn áp dụng các chất điện phân dựa trên MOF trong các lĩnh vực điện sắc (EC). Tuy nhiên, có những thách thức trong việc nhận ra độ trong suốt quang học cao của chúng để đáp ứng các yêu cầu quang học độc đáo của các thiết bị EC. Ở đây, một chất điện phân gel dựa trên MOF trong suốt (MGE) được chứng minh thông qua việc kết hợp 2-methylimidazole giữa các tinh thể nano MOF để ngăn chặn sự tán xạ ánh sáng mạnh của các tinh thể nano MOF. Kết quả là, chất điện phân gel cho thấy độ truyền sáng trung bình được cải thiện khoảng 82,2% so với chất điện phân MOF không có 2-methylimidazole (khoảng 59,2%). Ngoài ra, do các kênh lớn được thiết kế trong cấu trúc MOF xốp, chất điện phân gel thể hiện độ dẫn ion cao là 2,66 × 10-3 S cm-1. Cuối cùng, chúng tôi đã sử dụng MGE trong suốt để lắp ráp hai loại (cứng và mềm) thiết bị EC trạng thái bán rắn dựa trên WO3 vô cơ và poly(3,4-ethylenedioxythiophene) (PEDOT) hữu cơ. Cả hai thiết bị đều cho thấy hiệu suất EC tuyệt vời và các thiết bị linh hoạt thể hiện độ ổn định cơ học cao ở trạng thái uốn cong hoặc thậm chí sau khi bị cắt và đục lỗ, thúc đẩy các ứng dụng chung của MGE trong suốt của chúng trong các lĩnh vực EC."} +{"idx": 1870108, "en": "The brainstem, the core of the central nervous system, plays a vital role in controlling arterial blood pressure and its elevation of hypertension subtypes, especially essential hypertension. Integrative metabolic and proteomic profiling was performed on the brainstem samples of 11 week old spontaneously hypertensive rats (SHRs) and age-matched normotensive Wistar rats, using hydrophilic interaction liquid chromatography quadrupole/time-of-flight mass spectrometry (HILIC-Q/TOFMS) (PeptideAtlas: PASS01621) and nano-liquid chromatography-high-resolution-MS (nano-LC-high-resolution) combined with quantitative tandem mass tags (ProteomeXchange: PXD021210). The results showed a potentially significant measure of metabolic disorders in the brainstem of SHRs, including purine and pyrimidine metabolism and carnitine and acylcarnitine deficiency. By integrating the differential metabolites (VIP > 1 and p < 0.1) with the differentially expressed proteins (>1.2-fold and p < 0.05), the results revealed aberrant insulin signaling in the brainstem of SHRs, including reduced carnitine and acetylcarnitine; increased arginine; and increased flotillin-1 (FLOT1), hemoglobin subunit alpha-1/2, and hemoglobin subunit beta-2 proteins verified by the parallel reaction monitoring analysis (PeptideAtlas: PASS01622). The aberrant insulin signaling pathway in the brainstem of SHRs might help explain the correlation between essential hypertension and insulin resistance. These findings on the brainstem of SHRs could provide new insights into the dysregulation of the central nervous system in hypertension, especially as it relates to metabolite and protein levels.", "gemini": "Thân não, là lõi của hệ thần kinh trung ương, đóng vai trò quan trọng trong việc kiểm soát huyết áp động mạch và sự tăng huyết áp của các phân nhóm tăng huyết áp, đặc biệt là tăng huyết áp vô căn. Hồ sơ chuyển hóa và protein học tích hợp đã được thực hiện trên các mẫu thân não của chuột tăng huyết áp tự phát (SHR) 11 tuần tuổi và chuột Wistar bình thường cùng độ tuổi, sử dụng sắc ký lỏng tương tác ưa nước khối phổ tứ cực/thời gian bay (HILIC-Q/TOFMS) (PeptideAtlas: PASS01621) và sắc ký lỏng nano-độ phân giải cao kết hợp với các thẻ khối lượng song song định lượng (ProteomeXchange: PXD021210). Kết quả cho thấy một thước đo có ý nghĩa tiềm tàng về rối loạn chuyển hóa ở thân não của SHR, bao gồm chuyển hóa purin và pyrimidine và tình trạng thiếu carnitine và acylcarnitine. Bằng cách tích hợp các chất chuyển hóa khác biệt (VIP > 1 và p < 0,1) với các protein biểu hiện khác biệt (>1,2 lần và p < 0,05), kết quả cho thấy tín hiệu insulin bất thường ở thân não của SHR, bao gồm giảm carnitine và acetylcarnitine; tăng arginin; và tăng protein flotillin-1 (FLOT1), tiểu đơn vị hemoglobin alpha-1/2 và tiểu đơn vị hemoglobin beta-2 được xác minh bằng phân tích theo dõi phản ứng song song (PeptideAtlas: PASS01622). Con đường tín hiệu insulin bất thường ở thân não của SHR có thể giúp giải thích mối tương quan giữa tăng huyết áp vô căn và tình trạng kháng insulin. Những phát hiện này về thân não của SHR có thể cung cấp những hiểu biết mới về tình trạng mất điều hòa của hệ thần kinh trung ương trong tăng huyết áp, đặc biệt là khi liên quan đến nồng độ chất chuyển hóa và protein."} +{"idx": 1870109, "en": "Herein, by applying visible-light photoredox catalysis, we have now achieved the first example of catalytic dehydrogenative coupling of phosphines and thiophenols that proceeds at room temperature. Key to our success is the use of benzaldehyde as a soft oxidant, which avoids the issue of phosphine oxidation. Furthermore, we observed the unexpected dealkylative coupling of secondary and tertiary alkylphosphine with thiophenols.", "gemini": "Trong đây, bằng cách áp dụng xúc tác quang khử bằng ánh sáng khả kiến, chúng tôi hiện đã đạt được ví dụ đầu tiên về phản ứng ghép đôi khử hydro xúc tác của phosphin và thiophenol diễn ra ở nhiệt độ phòng. Chìa khóa thành công của chúng tôi là sử dụng benzaldehyd làm chất oxy hóa nhẹ, giúp tránh được vấn đề oxy hóa phosphin. Hơn nữa, chúng tôi quan sát thấy phản ứng ghép đôi khử alkyl bất ngờ của alkylphosphin bậc hai và bậc ba với thiophenol."} +{"idx": 1870110, "en": "Literature studies on interfacial tension versus temperature between normal alcohols and water show that it increases with temperature and exhibits a maximum value at a given temperature depending on the molecular weight of the alcohol. This very unusual behavior is supposedly accompanied by the formation of monolayers of alcohol molecules oriented preferentially at the interface, a structural issue not confirmed until now. We use molecular-based models for water and alcohols in combination with molecular dynamics simulations to provide physical insights, from a molecular perspective, into the structural and thermodynamic behavior at the liquid-liquid interfaces of aqueous solutions of alcohols.", "gemini": "Các nghiên cứu tài liệu về sức căng bề mặt so với nhiệt độ giữa rượu thông thường và nước cho thấy nó tăng theo nhiệt độ và thể hiện giá trị cực đại ở một nhiệt độ nhất định tùy thuộc vào trọng lượng phân tử của rượu. Hành vi rất bất thường này được cho là đi kèm với sự hình thành các lớp đơn phân tử của các phân tử rượu định hướng ưu tiên tại giao diện, một vấn đề về cấu trúc chưa được xác nhận cho đến nay. Chúng tôi sử dụng các mô hình dựa trên phân tử đối với nước và rượu kết hợp với mô phỏng động học phân tử để cung cấp thông tin vật lý, từ góc độ phân tử, về hành vi cấu trúc và nhiệt động lực học tại các giao diện lỏng-lỏng của các dung dịch nước của rượu."} +{"idx": 1870111, "en": "Infrared vibrational excitation is a promising approach for gaining exceptional control of chemical reactions, in ways that cannot be attained via thermal or electronic excitation. Here, we report an unprecedented example of a bond-breaking/bond-forming reaction by vibrational excitation under matrix isolation conditions. Thiotropolone monomers were isolated in cryogenic argon matrices and characterized by infrared spectroscopy and vibrational computations (harmonic and anharmonic). Narrowband near-infrared irradiations tuned at frequencies of first CH stretching overtone (5940 cm-1) or combination modes (5980 cm-1) of the OH tautomer, the sole form of the compound that exists in the as-deposited matrices, led to its conversion into the SH tautomer. The tautomerization in the reverse direction was achieved by vibrational excitation of the SH tautomer with irradiation at 5947 or 5994 cm-1, corresponding to the frequencies of its CH stretching combination and overtone modes. This pioneer demonstration of bidirectional hydroxyl ↔ thiol tautomerization controlled by vibrational excitation creates prospects for new advances in vibrationally induced chemistry.", "gemini": "Kích thích rung hồng ngoại là một phương pháp đầy hứa hẹn để kiểm soát đặc biệt các phản ứng hóa học theo những cách không thể đạt được thông qua kích thích nhiệt hoặc điện tử. Ở đây, chúng tôi báo cáo một ví dụ chưa từng có về phản ứng phá liên kết/tạo liên kết bằng cách kích thích rung trong điều kiện cô lập ma trận. Các monome thiotropolone được cô lập trong các ma trận argon lạnh và được đặc trưng bằng quang phổ hồng ngoại và tính toán rung (hài hòa và phi hài hòa). Các bức xạ cận hồng ngoại hẹp được điều chỉnh ở tần số của âm bội kéo giãn CH đầu tiên (5940 cm-1) hoặc các chế độ kết hợp (5980 cm-1) của đồng phân tautome OH, dạng duy nhất của hợp chất tồn tại trong các ma trận lắng đọng, dẫn đến chuyển đổi thành đồng phân tautome SH. Sự đồng phân hóa theo hướng ngược lại đạt được bằng cách kích thích rung của đồng phân tautome SH với bức xạ ở 5947 hoặc 5994 cm-1, tương ứng với tần số của các chế độ kết hợp và âm bội kéo giãn CH của nó. Biểu diễn tiên phong về sự đồng phân hóa hydroxyl ↔ thiol hai chiều được kiểm soát bằng cách kích thích rung tạo ra triển vọng cho những tiến bộ mới trong hóa học được tạo ra bằng rung động."} +{"idx": 1870112, "en": "Co-cultivation has been used as a promising tool to turn on or up-regulate cryptic biosynthetic pathways for microbial natural product discovery. Recently, a modified culturing strategy similar to co-cultivation was investigated, where heat-killed inducer cultures were supplemented to the culture medium of producer fermentations to induce cryptic pathways. In the present study, the repeatability and effectiveness of both methods in turning on cryptic biosynthetic pathways were unbiasedly assessed using UHPLC-HRESIMS-based metabolomics analysis. Both induction methods had good repeatability, and they resulted in very different induced metabolites from the tested producers. Co-cultivation generated more induced mass features than the heat-killed inducer cultures, while both methods resulted in the induction of mass features not observed using the other induction method. As examples, pathways leading to two new natural products, N-carbamoyl-2-hydroxy-3-methoxybenzamide (1) and carbazoquinocin G (5), were induced and up-regulated through co-culturing a producer Streptomyces sp. RKND-216 with inducers Alteromonas sp. RKMC-009 and M. smegmatis ATCC 120515, respectively.", "gemini": "Nuôi cấy chung đã được sử dụng như một công cụ đầy hứa hẹn để bật hoặc điều chỉnh tăng các con đường sinh tổng hợp bí ẩn để phát hiện ra sản phẩm tự nhiên của vi khuẩn. Gần đây, một chiến lược nuôi cấy được sửa đổi tương tự như nuôi cấy chung đã được nghiên cứu, trong đó các nuôi cấy gây cảm ứng đã chết vì nhiệt được bổ sung vào môi trường nuôi cấy của quá trình lên men của nhà sản xuất để tạo ra các con đường bí ẩn. Trong nghiên cứu hiện tại, khả năng lặp lại và hiệu quả của cả hai phương pháp trong việc bật các con đường sinh tổng hợp bí ẩn đã được đánh giá một cách không thiên vị bằng cách sử dụng phân tích chuyển hóa dựa trên UHPLC-HRESIMS. Cả hai phương pháp cảm ứng đều có khả năng lặp lại tốt và chúng dẫn đến các chất chuyển hóa được cảm ứng rất khác nhau từ các nhà sản xuất được thử nghiệm. Nuôi cấy chung tạo ra nhiều đặc điểm khối lượng được cảm ứng hơn so với các nuôi cấy gây cảm ứng đã chết vì nhiệt, trong khi cả hai phương pháp đều dẫn đến sự cảm ứng của các đặc điểm khối lượng không được quan sát thấy khi sử dụng phương pháp cảm ứng khác. Ví dụ, các con đường dẫn đến hai sản phẩm tự nhiên mới, N-carbamoyl-2-hydroxy-3-methoxybenzamide (1) và carbazoquinocin G (5), đã được cảm ứng và điều chỉnh tăng thông qua nuôi cấy chung một nhà sản xuất Streptomyces sp. RKND-216 với chất gây cảm ứng Alteromonas sp. RKMC-009 và M. smegmatis ATCC 120515."} +{"idx": 1870113, "en": "BACKGROUND. Performance-based multitasking assessments may be more sensitive than cognitive screens to detect executive dysfunction after a mild stroke. PURPOSE. This cross-sectional study examined inter-rater reliability and preliminary convergent and discriminant validity of the Front Desk Duty Test (FDDT). METHOD. Adults with mild stroke (n = 35) and community controls (n = 33) were administered the FDDT and other measures of executive functions. FINDINGS. Inter-rater reliability of the FDDT subscores were high (ICC 0.971-0.999). There were weak but statistically significant correlations between participants' scores on some of the executive function tests and some FDDT subscores. Participants with mild stroke and community controls had statistically significant different FDDT scores (performance accuracy p = .006; performance time, p = .033), with rank order FDDT score patterns across community controls, participants with mild stroke who passed the executive function screen, and participants with mild stroke who failed the executive function screen. IMPLICATIONS. Preliminary validation results suggest that the FDDT warrants further study.", "gemini": "LÝ LỊCH. Đánh giá đa nhiệm dựa trên hiệu suất có thể nhạy hơn so với sàng lọc nhận thức để phát hiện rối loạn chức năng điều hành sau một cơn đột quỵ nhẹ. MỤC ĐÍCH. Nghiên cứu cắt ngang này đã kiểm tra độ tin cậy giữa các đánh giá viên và độ hợp lệ hội tụ và phân biệt sơ bộ của Bài kiểm tra nhiệm vụ lễ tân (FDDT). PHƯƠNG PHÁP. Người lớn bị đột quỵ nhẹ (n = 35) và nhóm đối chứng cộng đồng (n = 33) đã được thực hiện FDDT và các biện pháp khác về chức năng điều hành. KẾT QUẢ. Độ tin cậy giữa các đánh giá viên của các điểm phụ FDDT là cao (ICC 0,971-0,999). Có sự tương quan yếu nhưng có ý nghĩa thống kê giữa điểm số của người tham gia trong một số bài kiểm tra chức năng điều hành và một số điểm phụ FDDT. Những người tham gia bị đột quỵ nhẹ và nhóm đối chứng cộng đồng có điểm FDDT khác biệt có ý nghĩa thống kê (độ chính xác của hiệu suất p = 0,006; thời gian thực hiện, p = 0,033), với thứ hạng các mẫu điểm FDDT trên nhóm đối chứng cộng đồng, những người tham gia bị đột quỵ nhẹ vượt qua sàng lọc chức năng điều hành và những người tham gia bị đột quỵ nhẹ không vượt qua được sàng lọc chức năng điều hành. Ý NGHĨA. Kết quả xác nhận sơ bộ cho thấy FDDT cần được nghiên cứu thêm."} +{"idx": 1870114, "en": "Aim: To update overall survival (OS) results from a previous network meta analysis comparing the relative clinical efficacy of epidermal growth factor receptor-targeted tyrosine kinase inhibitors (EGFR TKIs) for EGFR mutation positive (EGFR+) advanced non-small-cell lung cancer (NSCLC). Materials & methods: A Bayesian network meta analysis was conducted using updated/mature randomized controlled trial OS results in response to first-line EGFR TKI therapies. Results: Dacomitinib showed a numerical improvement of OS relative to other EGFR TKIs: afatinib (hazard ratio [HR]: 0.87; 95% credible interval [CrI]: 0.61-1.24), erlotinib (HR: 0.79; 95% CrI: 0.44-1.42), gefitinib (HR: 0.75; 95% CrI: 0.59-0.95) and osimertinib (HR: 0.94; 95% CrI: 0.68-1.29). Conclusion: Dacomitinib should be considered as a first-line treatment option for patients diagnosed with advanced EGFR+ NSCLC.", "gemini": "Mục đích: Cập nhật kết quả sống toàn thể (OS) từ phân tích meta mạng trước đó so sánh hiệu quả lâm sàng tương đối của các chất ức chế tyrosine kinase nhắm vào thụ thể yếu tố tăng trưởng biểu bì (EGFR TKI) đối với ung thư phổi không tế bào nhỏ (NSCLC) tiến triển có đột biến EGFR dương tính (EGFR +). Vật liệu & phương pháp: Một phân tích meta mạng Bayesian đã được thực hiện bằng cách sử dụng kết quả OS của thử nghiệm có đối chứng ngẫu nhiên đã cập nhật/trưởng thành để đáp ứng với các liệu pháp EGFR TKI一线. Kết quả: Dacomitinib cho thấy sự cải thiện về mặt số liệu của OS so với các EGFR TKI khác: afatinib (tỷ lệ nguy cơ [HR]: 0,87; khoảng tin cậy đáng tin cậy 95% [CrI]: 0,61-1,24), erlotinib (HR: 0,79; 95% CrI: 0,44-1,42), gefitinib (HR: 0,75; 95% CrI: 0,59-0,95) và osimertinib (HR: 0,94; 95% CrI: 0,68-1,29). Kết luận: Dacomitinib nên được xem xét như một lựa chọn điều trị一线 cho những bệnh nhân được chẩn đoán mắc NSCLC EGFR + tiến triển."} +{"idx": 1870115, "en": "INTRODUCTION Indwelling pleural catheters (IPC) are effective at palliating benign and malignant pleural effusions (MPE). They have also been found to be cost effective from a third-party payor perspective. Little is known of the impact IPCs have on patient-centered quality of life outcomes such as financial burden and patient and caregiver burden. We performed a cross-sectional survey study evaluating the impact of IPCs on multiple patient and caregiver quality of life metrics. METHODS Patients ≥ 18 years old with an IPC in place for 2 months were eligible. Twenty patients were recruited over a 10-month period. Patients completed the CDC-Health Related Quality of Life (HRQOL)-4 and a HRQOL-financial questionnaire. The primary objective was to describe the socio-economic impact of IPCs. Demographic and IPC specific data were collected. Descriptive statistics were used. RESULTS The mean (SD) age was 64.3 (0.70). The indication was MPE in 19/20. All patients had medical insurance. Medicare or Medicaid (CMS) comprised 10/20 of payors. The median (IQR) copay for private insurers was $238.45 (72-875); 11/20 had additional costs related to the IPC; 4/20 had significant life changes after the IPC; 17/20 received assistance from a non-paid caregiver; 6/20 patients could not do activities because of the IPC and this negatively impacted QOL in 3/6 of those patients. CONCLUSION Patients with IPCs may experience negative life consequences, incur additional medical expenses, and require assistance from a non-paid caregiver. Activities may be negatively impacted by IPC. Discussion of alternative means of symptom palliation and pleurodesis would be beneficial.", "gemini": "GIỚI THIỆU Các ống thông màng phổi cố định (IPC) có hiệu quả trong việc giảm nhẹ các dịch màng phổi lành tính và ác tính (MPE). Chúng cũng được phát hiện là hiệu quả về mặt chi phí theo quan điểm của bên thứ ba. Người ta biết rất ít về tác động của IPC đối với các kết quả về chất lượng cuộc sống tập trung vào bệnh nhân như gánh nặng tài chính và gánh nặng cho bệnh nhân và người chăm sóc. Chúng tôi đã thực hiện một nghiên cứu khảo sát cắt ngang để đánh giá tác động của IPC đối với nhiều số liệu về chất lượng cuộc sống của bệnh nhân và người chăm sóc. PHƯƠNG PHÁP Những bệnh nhân ≥ 18 tuổi đã đặt IPC trong 2 tháng đủ điều kiện. Hai mươi bệnh nhân đã được tuyển dụng trong khoảng thời gian 10 tháng. Bệnh nhân đã hoàn thành CDC-Health Related Quality of Life (HRQOL) -4 và bảng câu hỏi tài chính HRQOL. Mục tiêu chính là mô tả tác động kinh tế xã hội của IPC. Dữ liệu nhân khẩu học và cụ thể của IPC đã được thu thập. Các số liệu thống kê mô tả đã được sử dụng. KẾT QUẢ Độ tuổi trung bình (SD) là 64,3 (0,70). Chỉ định là MPE ở 19/20. Tất cả bệnh nhân đều có bảo hiểm y tế. Medicare hoặc Medicaid (CMS) chiếm 10/20 người trả tiền. Khoản đồng trả trung bình (IQR) cho các công ty bảo hiểm tư nhân là 238,45 đô la (72-875); 11/20 có thêm chi phí liên quan đến IPC; 4/20 có những thay đổi đáng kể về cuộc sống sau khi đặt IPC; 17/20 nhận được sự trợ giúp từ người chăm sóc không được trả lương; 6/20 bệnh nhân không thể làm các hoạt động vì IPC và điều này ảnh hưởng tiêu cực đến QOL ở 3/6 bệnh nhân đó. KẾT LUẬN Những bệnh nhân có IPC có thể gặp phải những hậu quả tiêu cực trong cuộc sống, phải chịu thêm chi phí y tế và cần sự hỗ trợ từ người chăm sóc không được trả lương. Các hoạt động có thể bị ảnh hưởng tiêu cực bởi IPC. Việc thảo luận về các phương tiện thay thế để giảm nhẹ triệu chứng và làm màng phổi dày lên sẽ có lợi."} +{"idx": 1870116, "en": "The majority of states require the signature of a surrogate decision maker on a POLST form for a patient who lacks decisional capacity. While commendable in its intention to ensure informed consent, in some cases this may lead the surrogate to feel that they are signing their loved one's \"death warrant,\" adding to their emotional and spiritual distress. In this paper we argue that such a signature should be recommended rather than required, as it is neither a sufficient nor necessary condition of informed consent. Additional steps-such as requiring the attestation and documentation of the signing health care professional that verbal consent was fully informed and voluntary-can achieve the ultimate goal of respecting patient autonomy without adding to the surrogate's burden.", "gemini": "Đa số các tiểu bang yêu cầu chữ ký của người ra quyết định thay thế trên biểu mẫu POLST cho bệnh nhân không có khả năng ra quyết định. Mặc dù đáng khen ngợi về ý định đảm bảo sự đồng ý có thông tin, nhưng trong một số trường hợp, điều này có thể khiến người thay thế cảm thấy rằng họ đang ký \"giấy báo tử\" cho người thân yêu của mình, làm tăng thêm nỗi đau về mặt tình cảm và tinh thần của họ. Trong bài báo này, chúng tôi lập luận rằng một chữ ký như vậy nên được khuyến nghị chứ không phải bắt buộc, vì nó không phải là điều kiện đủ cũng không phải là điều kiện cần của sự đồng ý có thông tin. Các bước bổ sung - chẳng hạn như yêu cầu xác nhận và ghi chép của chuyên gia chăm sóc sức khỏe ký tên rằng sự đồng ý bằng lời là hoàn toàn có thông tin và tự nguyện - có thể đạt được mục tiêu cuối cùng là tôn trọng quyền tự chủ của bệnh nhân mà không làm tăng thêm gánh nặng cho người thay thế."} +{"idx": 1870117, "en": "BACKGROUND Denosumab is a human monoclonal antibody that has been used successfully in the treatment of giant cell tumors of bone. These tumors are rare and, in principle, benign, but they are highly aggressive, locally advanced, osteolytic bone tumors that can metastasize to the lungs. Denosumab is an effective treatment when these tumors cannot be surgically removed or when surgical resection is likely to lead to severe morbidity (eg, loss of limbs or joints). The aim of this systematic review and meta-analysis was to investigate patients with giant cell tumors of bone who experienced tumor progression during treatment with denosumab and to compare them with patients who experienced reduction of their giant cell tumors of bone during treatment with denosumab. METHODS Embase, Cochrane Library, and MEDLINE/PubMed databases were searched for trials submitted by January 7, 2020, that reported the efficacy and safety of denosumab in patients with giant cell tumors of bone. RESULTS Sixty studies were reviewed, involving a total of 1074 patients who had giant cell tumors of bone and were treated with denosumab. Of the 60 studies, 58% of the patients were from case series studies, 39% from open-label phase II studies, and 3% from case reports. The response rate for denosumab as a treatment for giant cell tumors of bone was 97.5%, with statistical significance (P < .0001). Pain in the limbs was statistically the most common adverse event for denosumab treatment in case series studies (P < .0001). No treatment-related deaths occurred in the reviewed studies. CONCLUSION Cumulative evidence supports the addition of surgery to optimal medical therapy with denosumab to reduce tumor size, clinical symptoms, and mortality among patients with giant cell tumors of bone.", "gemini": "BỐI CẢNH Denosumab là một kháng thể đơn dòng ở người đã được sử dụng thành công trong điều trị u tế bào khổng lồ ở xương. Những khối u này rất hiếm và về nguyên tắc là lành tính, nhưng chúng là những khối u xương phá hủy xương, tiến triển tại chỗ rất hung dữ và có thể di căn đến phổi. Denosumab là một phương pháp điều trị hiệu quả khi không thể phẫu thuật cắt bỏ những khối u này hoặc khi cắt bỏ phẫu thuật có khả năng dẫn đến bệnh tật nghiêm trọng (ví dụ như mất chi hoặc khớp). Mục đích của tổng quan hệ thống và phân tích tổng hợp này là nhằm nghiên cứu những bệnh nhân mắc u tế bào khổng lồ ở xương đã trải qua tiến triển khối u trong quá trình điều trị bằng denosumab và so sánh họ với những bệnh nhân đã trải qua quá trình giảm u tế bào khổng lồ ở xương trong quá trình điều trị bằng denosumab. PHƯƠNG PHÁP Các cơ sở dữ liệu Embase, Thư viện Cochrane và MEDLINE/PubMed đã được tìm kiếm các thử nghiệm được nộp trước ngày 7 tháng 1 năm 2020, báo cáo về hiệu quả và tính an toàn của denosumab ở những bệnh nhân mắc u tế bào khổng lồ ở xương. KẾT QUẢ Sáu mươi nghiên cứu đã được xem xét, bao gồm tổng cộng 1074 bệnh nhân mắc u tế bào khổng lồ ở xương và được điều trị bằng denosumab. Trong số 60 nghiên cứu, 58% bệnh nhân đến từ các nghiên cứu về chuỗi trường hợp, 39% đến từ các nghiên cứu giai đoạn II nhãn mở và 3% đến từ các báo cáo trường hợp. Tỷ lệ đáp ứng với denosumab như một phương pháp điều trị u tế bào khổng lồ ở xương là 97,5%, có ý nghĩa thống kê (P < 0,0001). Đau ở các chi là tác dụng phụ phổ biến nhất về mặt thống kê đối với phương pháp điều trị bằng denosumab trong các nghiên cứu về chuỗi trường hợp (P < 0,0001). Không có trường hợp tử vong liên quan đến điều trị nào xảy ra trong các nghiên cứu được xem xét. KẾT LUẬN Bằng chứng tích lũy ủng hộ việc bổ sung phẫu thuật vào liệu pháp y tế tối ưu bằng denosumab để giảm kích thước khối u, các triệu chứng lâm sàng và tỷ lệ tử vong ở những bệnh nhân mắc u tế bào khổng lồ ở xương."} +{"idx": 1870118, "en": "Cancer cells exist within a complex spatially structured ecosystem composed of resources and different cell types. As the selective pressures imposed by this environment determine the fate of cancer cells, an improved understanding of how this ecosystem evolves will better elucidate how tumors grow and respond to therapy. State of the art imaging methods can now provide highly resolved descriptions of the microenvironment, yielding the data required for a thorough study of its role in tumor growth and treatment resistance. The field of landscape ecology has been studying such species-environment relationship for decades, and offers many tools and perspectives that cancer researchers could greatly benefit from. Here, we discuss one such tool, species distribution modeling (SDM), that has the potential to, among other things, identify critical environmental factors that drive tumor evolution and predict response to therapy. SDMs only scratch the surface of how ecological theory and methods can be applied to cancer, and we believe further integration will take cancer research in exciting new and productive directions. Significance: Here we describe how species distribution modeling can be used to quantitatively describe the complex relationship between tumor cells and their microenvironment. Such a description facilitates a deeper understanding of cancers eco-evolutionary dynamics, which in turn sheds light on the factors that drive tumor growth and response to treatment.", "gemini": "Các tế bào ung thư tồn tại trong một hệ sinh thái có cấu trúc không gian phức tạp bao gồm các nguồn lực và các loại tế bào khác nhau. Vì áp lực chọn lọc do môi trường này áp đặt quyết định số phận của các tế bào ung thư, nên việc hiểu rõ hơn về cách hệ sinh thái này tiến hóa sẽ giúp làm sáng tỏ hơn cách các khối u phát triển và phản ứng với liệu pháp. Các phương pháp hình ảnh hiện đại hiện có thể cung cấp các mô tả có độ phân giải cao về môi trường vi mô, tạo ra dữ liệu cần thiết cho một nghiên cứu toàn diện về vai trò của nó trong sự phát triển của khối u và khả năng kháng trị. Lĩnh vực sinh thái cảnh quan đã nghiên cứu mối quan hệ giữa loài và môi trường như vậy trong nhiều thập kỷ và cung cấp nhiều công cụ và góc nhìn mà các nhà nghiên cứu ung thư có thể hưởng lợi rất nhiều. Tại đây, chúng tôi thảo luận về một công cụ như vậy, mô hình phân bố loài (SDM), có khả năng xác định các yếu tố môi trường quan trọng thúc đẩy quá trình tiến hóa của khối u và dự đoán phản ứng với liệu pháp. Các SDM chỉ mới khám phá bề nổi về cách áp dụng lý thuyết và phương pháp sinh thái vào ung thư và chúng tôi tin rằng việc tích hợp sâu hơn sẽ đưa nghiên cứu ung thư theo những hướng mới và hiệu quả. Ý nghĩa: Tại đây, chúng tôi mô tả cách mô hình phân bố loài có thể được sử dụng để mô tả định lượng mối quan hệ phức tạp giữa các tế bào khối u và môi trường vi mô của chúng. Mô tả như vậy tạo điều kiện cho việc hiểu sâu hơn về động lực tiến hóa sinh thái của bệnh ung thư, từ đó làm sáng tỏ các yếu tố thúc đẩy sự phát triển của khối u và phản ứng với điều trị."} +{"idx": 1870119, "en": "BACKGROUND The introduction of insulin pumps with bolus calculators (BCs) has improved glycemic outcomes and quality of life for those with type 1 diabetes. Despite the increased reliance on BCs, the formulas used to derive recommended boluses are not standardized. Our objective was to examine whether recommendations from different pump BCs vary significantly for identical clinical scenarios. METHODS Three commercially available insulin pump BCs were programmed with identical settings and then presented with combinations of blood glucose (BG) and carbohydrates (CHOs) to generate a 4-unit bolus. At one- and two-hour time points, while there was insulin-on-board (IOB) present, we simulated various BG and CHO scenarios in order to compare BC-recommended doses. RESULTS Differences in suggested doses were noted between BCs, as well as within the same brand. The greatest variation was apparent when BG was below target. Doses suggested by one BC varied depending on whether the IOB resulted from a previous dose given for BG or CHO, while the other two BCs adjusted for total IOB regardless of the source. CONCLUSIONS In this simulation study, there were large differences in recommended doses between BCs due to the unique way each manufacturer incorporates IOB into their formulas as well as the pharmacokinetics used to derive the IOB amount. Providers should be aware that identical pump settings will result in a different dose recommendation for each pump brand and advise patients accordingly.", "gemini": "BỐI CẢNH Việc đưa máy bơm insulin có máy tính bolus (BC) vào sử dụng đã cải thiện kết quả đường huyết và chất lượng cuộc sống cho những người mắc bệnh tiểu đường týp 1. Mặc dù ngày càng phụ thuộc nhiều hơn vào BC, nhưng các công thức được sử dụng để tính toán bolus được khuyến nghị không được chuẩn hóa. Mục tiêu của chúng tôi là kiểm tra xem các khuyến nghị từ các BC bơm khác nhau có khác biệt đáng kể đối với các tình huống lâm sàng giống hệt nhau hay không. PHƯƠNG PHÁP Ba BC bơm insulin thương mại đã được lập trình với các cài đặt giống hệt nhau và sau đó được đưa ra các kết hợp giữa lượng đường trong máu (BG) và carbohydrate (CHO) để tạo ra một bolus 4 đơn vị. Tại các thời điểm một và hai giờ, trong khi có insulin trên boong (IOB), chúng tôi đã mô phỏng nhiều tình huống BG và CHO khác nhau để so sánh các liều khuyến nghị của BC. KẾT QUẢ Sự khác biệt về liều được đề xuất đã được ghi nhận giữa các BC, cũng như trong cùng một thương hiệu. Sự khác biệt lớn nhất là rõ ràng khi BG dưới mục tiêu. Liều lượng do một BC đề xuất thay đổi tùy thuộc vào việc IOB có phải là kết quả của liều trước đó được đưa ra cho BG hay CHO hay không, trong khi hai BC còn lại điều chỉnh cho IOB tổng thể bất kể nguồn gốc. KẾT LUẬN Trong nghiên cứu mô phỏng này, có sự khác biệt lớn về liều khuyến nghị giữa các BC do cách riêng mà mỗi nhà sản xuất kết hợp IOB vào công thức của họ cũng như dược động học được sử dụng để tính toán lượng IOB. Các nhà cung cấp nên biết rằng các cài đặt bơm giống hệt nhau sẽ dẫn đến khuyến nghị về liều lượng khác nhau cho từng thương hiệu bơm và tư vấn cho bệnh nhân phù hợp."} +{"idx": 1870120, "en": "RECOMMENDATION There is evidence indicating that the amount of bony correction performed in the setting of progressive collapsing foot deformity reconstructive surgery can be titrated within a recommended range for a variety of procedures. The typical range when performing a medial displacement calcaneal osteotomy should be 7 to 15 mm of medialization of the tuberosity. The typical range when performing an Evans lateral column lengthening should be 5 to 10 mm of a laterally based wedge in the anterior calcaneus. The typical range when performing a plantarflexion opening wedge osteotomy of the medial cuneiform (Cotton) osteotomy should be 5 to 10 mm of a dorsal wedge. LEVEL OF EVIDENCE Level V, consensus, expert opinion.", "gemini": "KHUYẾN NGHỊ Có bằng chứng cho thấy lượng chỉnh sửa xương được thực hiện trong phẫu thuật tái tạo biến dạng bàn chân sụp tiến triển có thể được chuẩn độ trong phạm vi khuyến nghị cho nhiều loại thủ thuật khác nhau. Phạm vi điển hình khi thực hiện cắt xương gót chân chuyển vị trong là từ 7 đến 15 mm về trong của củ xương. Phạm vi điển hình khi thực hiện kéo dài cột bên Evans là từ 5 đến 10 mm của nêm nền bên trong xương gót chân trước. Phạm vi điển hình khi thực hiện cắt xương nêm mở góc gấp gan bàn chân trong (Cotton) là từ 5 đến 10 mm của nêm lưng."} +{"idx": 1870121, "en": "PURPOSE Standard treatment for retained products of conception (RPOC) is dilation and curettage (D&C), however, this brings a risk of intrauterine synechiae and subsequent fertility issues. A treatment strategy to avoid D&C is medical management with misoprostol. The justification for misoprostol in this setting is extrapolated from miscarriage and termination literature, however, no studies have looked specifically in the setting of RPOC. The purpose of this study is to determine the efficacy of misoprostol as definitive management of RPOC. MATERIALS AND METHODS A retrospective cohort study was conducted from January 2016 to March 2017 at an Early Pregnancy Assessment Clinic. Patients diagnosed with RPOC with clinical symptoms and ultrasound findings of endometrial mass and/or doppler flow treated expectantly, medically with vaginal misoprostol or with a suction D&C underwent a chart review. Primary outcome was resolution of RPOC without surgical intervention. RESULTS AND CONCLUSIONS Of 1743 unique pregnancies, 189 women were diagnosed with RPOC. 34% (65/178) chose misoprostol for management of RPOC. Baseline demographics between the three treatment modalities (expectant, medical, surgical) were comparable. 65% (42/65) of patients who took misoprostol avoided D&C. Misoprostol is most effective (76%) in the setting of RPOC if the primary treatment for miscarriage was expectant management, with less success with primary medical management (44%) and primary surgical management (40%).", "gemini": "MỤC ĐÍCH Điều trị tiêu chuẩn đối với các sản phẩm thụ thai còn sót lại (RPOC) là nong và nạo (D&C), tuy nhiên, điều này có nguy cơ gây dính buồng tử cung và các vấn đề về khả năng sinh sản sau này. Một chiến lược điều trị để tránh D&C là điều trị nội khoa bằng misoprostol. Sự biện minh cho misoprostol trong bối cảnh này được suy ra từ các tài liệu về sảy thai và chấm dứt thai kỳ, tuy nhiên, không có nghiên cứu nào xem xét cụ thể trong bối cảnh RPOC. Mục đích của nghiên cứu này là xác định hiệu quả của misoprostol như một biện pháp điều trị dứt điểm đối với RPOC. VẬT LIỆU VÀ PHƯƠNG PHÁP Một nghiên cứu theo dõi hồi cứu đã được tiến hành từ tháng 1 năm 2016 đến tháng 3 năm 2017 tại Phòng khám đánh giá thai kỳ sớm. Những bệnh nhân được chẩn đoán mắc RPOC có các triệu chứng lâm sàng và các phát hiện siêu âm về khối nội mạc tử cung và/hoặc lưu lượng doppler được điều trị theo dõi, nội khoa bằng misoprostol âm đạo hoặc bằng D&C hút đã được xem xét lại biểu đồ. Kết quả chính là giải quyết RPOC mà không cần can thiệp phẫu thuật. KẾT QUẢ VÀ KẾT LUẬN Trong số 1743 ca mang thai riêng lẻ, có 189 phụ nữ được chẩn đoán mắc RPOC. 34% (65/178) đã chọn misoprostol để điều trị RPOC. Dữ liệu nhân khẩu học ban đầu giữa ba phương thức điều trị (theo dõi, nội khoa, phẫu thuật) là tương đương nhau. 65% (42/65) bệnh nhân dùng misoprostol đã tránh được D&C. Misoprostol có hiệu quả nhất (76%) trong bối cảnh RPOC nếu phương pháp điều trị sảy thai chính là theo dõi, ít thành công hơn với phương pháp điều trị nội khoa chính (44%) và phương pháp điều trị phẫu thuật chính (40%)."} +{"idx": 1870122, "en": "BACKGROUND Standard dapsone and clofazimine-containing multidrug therapy (MDT) for leprosy is limited by drug tolerability, which poses treatment adherence barriers. Although ofloxacin-based regimens are promising alternatives, current efficacy and safety data are limited, particularly outside of endemic areas. We evaluated treatment outcomes in patients with leprosy receiving ofloxacin-containing MDT (OMDT) at our center. METHODS We performed a retrospective chart review of patients treated for leprosy at our center over an 8-year period (2011-2019). Primary outcomes evaluated were clinical cure rate, occurrence of leprosy reactions, antibiotic-related adverse events, and treatment adherence. Analyses were descriptive; however, data were stratified by age, sex, spectrum of disease, region of origin, and treatment regimen, and odds ratios were reported to assess associations with adverse outcomes. RESULTS Over the enrolment period, 26 patients were treated with OMDT (n = 19 multibacillary, n = 7 paucibacillary), and none were treated with clofazimine-based standard MDT. At the time of analysis, 23 patients (88%) had completed their course of treatment, and all were clinically cured, while 3 (12%) were still on treatment. Eighteen patients (69%) experienced either ENL (n = 7, 27%), type 1 reactions (n = 7, 27%), or both (n = 4, 15%). No patients stopped ofloxacin due to adverse drug effects, and there were no cases of allergic hypersensitivity, tendinopathy or rupture, or C. difficile colitis. CONCLUSIONS We demonstrate a high cure rate and tolerability of OMDT in this small case series over an 8-year period, suggesting its viability as an alternative to standard clofazimine-containing MDT.", "gemini": "BỐI CẢNH Liệu pháp đa thuốc (MDT) tiêu chuẩn chứa dapsone và clofazimine để điều trị bệnh phong bị hạn chế bởi khả năng dung nạp thuốc, gây ra rào cản trong việc tuân thủ điều trị. Mặc dù các chế độ dựa trên ofloxacin là những lựa chọn thay thế đầy hứa hẹn, nhưng dữ liệu về hiệu quả và độ an toàn hiện tại còn hạn chế, đặc biệt là ở bên ngoài các khu vực lưu hành. Chúng tôi đã đánh giá kết quả điều trị ở những bệnh nhân mắc bệnh phong đang dùng MDT có chứa ofloxacin (OMDT) tại trung tâm của chúng tôi. PHƯƠNG PHÁP Chúng tôi đã tiến hành xem xét lại biểu đồ bệnh án hồi cứu của những bệnh nhân được điều trị bệnh phong tại trung tâm của chúng tôi trong khoảng thời gian 8 năm (2011-2019). Các kết cục chính được đánh giá là tỷ lệ khỏi bệnh trên lâm sàng, tỷ lệ xảy ra phản ứng phong, các tác dụng phụ liên quan đến kháng sinh và mức độ tuân thủ điều trị. Các phân tích mang tính mô tả; tuy nhiên, dữ liệu được phân tầng theo tuổi, giới tính, phổ bệnh, khu vực xuất xứ và phác đồ điều trị, và tỷ lệ mắc được báo cáo để đánh giá mối liên quan với các kết cục bất lợi. KẾT QUẢ Trong thời gian tuyển dụng, 26 bệnh nhân được điều trị bằng OMDT (n = 19 đa khuẩn, n = 7 ít khuẩn) và không có bệnh nhân nào được điều trị bằng MDT tiêu chuẩn có chứa clofazimine. Vào thời điểm phân tích, 23 bệnh nhân (88%) đã hoàn thành liệu trình điều trị của mình và tất cả đều khỏi bệnh trên lâm sàng, trong khi 3 bệnh nhân (12%) vẫn đang được điều trị. Mười tám bệnh nhân (69%) đã trải qua ENL (n = 7, 27%), phản ứng loại 1 (n = 7, 27%) hoặc cả hai (n = 4, 15%). Không có bệnh nhân nào ngừng dùng ofloxacin do tác dụng phụ của thuốc và không có trường hợp nào bị dị ứng quá mẫn, bệnh lý gân hoặc đứt gân, hoặc viêm đại tràng do C. difficile. KẾT LUẬN Chúng tôi chứng minh tỷ lệ khỏi bệnh và khả năng dung nạp cao của OMDT trong loạt ca bệnh nhỏ này trong khoảng thời gian 8 năm, cho thấy khả năng tồn tại của nó như một lựa chọn thay thế cho MDT tiêu chuẩn có chứa clofazimine."} +{"idx": 1870123, "en": "Aim: To analyze responses to first-line metastatic renal cell carcinoma (mRCC) treatment stratified by risk criteria. Patients & methods: Clinical trials and observational studies of patients aged ≥18 years, published January 2005-May 2019, were identified via Ovid from MEDLINE, EMBASE, the Cochrane Central Trials Register and the Cochrane Database of Systematic Reviews. Data extracted included progression-free survival (PFS), overall survival (OS) and objective response rate (ORR). Results: 47/1269 articles met eligibility criteria. Most studies stratified patients by International Metastatic RCC Database Consortium (n = 19) or Memorial Sloan Kettering Cancer Center (n = 21). PFS, OS and ORR varied according to risk group. Conclusion: Pembrolizumab + axitinib, ipilimumab + nivolumab and avelumab + axitinib were most effective across all risk groups. Favorable-risk patients benefit from sunitinib treatment.", "gemini": "Mục đích: Phân tích các phản ứng với phương pháp điều trị ung thư biểu mô tế bào thận di căn (mRCC)一线治疗， được phân tầng theo tiêu chí rủi ro. Bệnh nhân và phương pháp: Các thử nghiệm lâm sàng và nghiên cứu quan sát đối với bệnh nhân từ 18 tuổi trở lên, được công bố từ tháng 1 năm 2005 đến tháng 5 năm 2019, đã được xác định thông qua Ovid từ MEDLINE, EMBASE, Sổ đăng ký thử nghiệm trung ương Cochrane và Cơ sở dữ liệu Cochrane về các đánh giá có hệ thống. Dữ liệu được trích xuất bao gồm thời gian sống không tiến triển (PFS), thời gian sống toàn thể (OS) và tỷ lệ đáp ứng khách quan (ORR). Kết quả: 47/1269 bài báo đáp ứng các tiêu chí đủ điều kiện. Hầu hết các nghiên cứu phân tầng bệnh nhân theo Liên đoàn Cơ sở dữ liệu ung thư biểu mô tế bào thận di căn quốc tế (n = 19) hoặc Trung tâm ung thư Memorial Sloan Kettering (n = 21). PFS, OS và ORR thay đổi tùy theo nhóm rủi ro. Kết luận: Pembrolizumab + axitinib, ipilimumab + nivolumab và avelumab + axitinib có hiệu quả nhất trên tất cả các nhóm rủi ro. Bệnh nhân có nguy cơ thuận lợi được hưởng lợi từ phương pháp điều trị sunitinib."} +{"idx": 1870124, "en": "A positive fluid balance has been found to be deleterious in critically ill patients; however, the impact of early fluid balance, particularly on long-term outcomes, in critically ill patients with cancer remains unclear. We performed this retrospective study at a tertiary-care referral hospital with 1500 beds and 6 intensive care units (ICUs) in central Taiwan, and 942 patients with cancer admitted to ICUs during 2013 to 2016 were enrolled. The primary outcome was 1-year mortality. Cancer-related data were obtained from the cancer registry, and data during ICU admissions were retrieved from the electronic medical records. The association between fluid balance, which was represented by median and interquartile range, and 1-year mortality was determined by calculating the hazard ratio (HR) with 95% confidence interval (CI) using a multivariable Cox proportional hazards regression model. The in-hospital mortality rate was 22.9% (216 of 942), and the mortality within 1 year after the index ICU admission was 38.7% (365 of 942). Compared to survivors, nonsurvivors tended to have a higher Acute Physiology and Chronic Health Evaluation II score (24.1 ± 6.9 vs 20.5 ± 6.2, P < .01), a higher age (65.0 ± 14.4 vs 61.3 ± 14.3, P < .01), a higher serum creatinine (1.5 ± 1.3 vs 1.0 ± 1.0, P < .01), and a higher cumulative day 1 to 4 fluid balance (2669, 955-5005 vs 4103, 1268-7215 mL, P < .01). Multivariable Cox proportional hazards regression analysis found that cumulative day-4 fluid balance was independently associated with 1-year mortality (adj HR 1.227, 95% CI: 1.132-1.329). A positive day 1 to 4 cumulative fluid balance was associated with shorter 1-year survival in critically ill patients with cancer. Further studies are needed to validate this association.", "gemini": "Cân bằng dịch dương được phát hiện gây hại ở những bệnh nhân bệnh nặng; tuy nhiên, tác động của cân bằng dịch sớm, đặc biệt là đối với kết quả lâu dài, ở những bệnh nhân ung thư bệnh nặng vẫn chưa rõ ràng. Chúng tôi đã thực hiện nghiên cứu hồi cứu này tại một bệnh viện chuyển tuyến chăm sóc sức khỏe ba cấp với 1500 giường và 6 đơn vị chăm sóc đặc biệt (ICU) ở miền trung Đài Loan, và 942 bệnh nhân ung thư được nhập viện vào ICU trong giai đoạn 2013 đến 2016 đã được ghi danh. Kết quả chính là tỷ lệ tử vong sau 1 năm. Dữ liệu liên quan đến ung thư được lấy từ sổ đăng ký ung thư và dữ liệu trong thời gian nhập viện ICU được lấy từ hồ sơ bệnh án điện tử. Sự liên quan giữa cân bằng dịch, được biểu thị bằng giá trị trung bình và phạm vi liên tứ phân vị, và tỷ lệ tử vong sau 1 năm được xác định bằng cách tính tỷ lệ nguy cơ (HR) với khoảng tin cậy 95% (CI) bằng cách sử dụng mô hình hồi quy tỷ lệ nguy cơ theo tỷ lệ Cox đa biến. Tỷ lệ tử vong trong viện là 22,9% (216/942) và tỷ lệ tử vong trong vòng 1 năm sau khi nhập viện ICU là 38,7% (365/942). So với những người sống sót, những người không sống sót có xu hướng có điểm Đánh giá sinh lý cấp tính và sức khỏe mãn tính II cao hơn (24,1 ± 6,9 so với 20,5 ± 6,2, P < 0,01), tuổi cao hơn (65,0 ± 14,4 so với 61,3 ± 14,3, P < 0,01), creatinin huyết thanh cao hơn (1,5 ± 1,3 so với 1,0 ± 1,0, P < 0,01) và cân bằng dịch tích lũy ngày 1 đến ngày 4 cao hơn (2669, 955-5005 so với 4103, 1268-7215 mL, P < 0,01). Phân tích hồi quy tỷ lệ nguy cơ theo tỷ lệ Cox đa biến cho thấy cân bằng dịch ngày 4 tích lũy có liên quan độc lập với tỷ lệ tử vong sau 1 năm (HR điều chỉnh 1,227, 95% CI: 1,132-1,329). Cân bằng dịch tích lũy ngày 1 đến ngày 4 dương liên quan đến thời gian sống sót sau 1 năm ngắn hơn ở những bệnh nhân ung thư bệnh nặng. Cần có thêm các nghiên cứu để xác nhận mối liên quan này."} +{"idx": 1870125, "en": "Aim: This study aims to investigate the significance of a micropapillary pattern in stage IIIA-N2 lung adenocarcinoma after adjuvant chemoradiotherapy. Patients & methods: A total of 257 patients with stage IIIA-N2 lung adenocarcinoma were enrolled in this study. Patients were classified into three groups based on the proportion of micropapillary components: micropapillary negative, micropapillary minor component and micropapillary predominant component. Results: The micropapillary predominant group had the shortest median disease-free survival and overall survival times compared with the micropapillary minor component and micropapillary negative groups (median overall survival time: 54 months vs 64 months vs not reached; p = 0.004). Furthermore, the micropapillary pattern was an independent prognostic factor for disease-free survival and overall survival (p < 0.05). Conclusion: The micropapillary pattern of IIIA-N2 lung adenocarcinoma is related to worse prognosis.", "gemini": "Mục tiêu: Nghiên cứu này nhằm mục đích tìm hiểu ý nghĩa của mô hình vi nhú ở ung thư biểu mô tuyến phổi giai đoạn IIIA-N2 sau hóa xạ trị bổ trợ. Bệnh nhân và phương pháp: Tổng cộng 257 bệnh nhân ung thư biểu mô tuyến phổi giai đoạn IIIA-N2 đã được ghi danh vào nghiên cứu này. Bệnh nhân được chia thành ba nhóm dựa trên tỷ lệ các thành phần vi nhú: vi nhú âm tính, thành phần vi nhú nhỏ và thành phần vi nhú chiếm ưu thế. Kết quả: Nhóm vi nhú chiếm ưu thế có thời gian sống không bệnh trung bình và thời gian sống toàn thể ngắn nhất so với nhóm thành phần vi nhú nhỏ và nhóm vi nhú âm tính (thời gian sống toàn thể trung bình: 54 tháng so với 64 tháng so với chưa đạt; p = 0,004). Hơn nữa, mô hình vi nhú là một yếu tố tiên lượng độc lập đối với thời gian sống không bệnh và thời gian sống toàn thể (p < 0,05). Kết luận: Mô hình vi nhú của ung thư biểu mô tuyến phổi IIIA-N2 có liên quan đến tiên lượng xấu hơn."} +{"idx": 1870126, "en": "OBJECTIVES This study aimed to explore health professional, patient, family, and caregiver perceptions of palliative care, availability of palliative care services to patients across South Dakota, and consistency and quality of palliative care delivery. METHODS Six focus groups were conducted over two months. Participants included interprofessional healthcare team members, patients, family members of patients, and caregivers. Individuals with palliative care experiences or interest in palliative care were invited to participate. Recruitment strategies included emails, flyers, and direct contact by members of the Network. Snowball sampling was used to recruit participants. RESULTS Forty-six participants included patients, family members, caregivers and interprofessional health care team members. Most participants were Caucasian (93.3%) and female (80%). Six primary themes emerged: Need for guidance toward the development of a holistic statewide palliative care model; Poor conceptual understanding and awareness; Insufficient resources to implement complete care in all South Dakota communities; Disparities in the availability and provision of care services in rural SD communities; Need for relationship and connection with palliative care team; and Secondary effects of palliative care on patients/family/caregivers and interprofessional healthcare team members. Significance of Results: Disproportionate access is a principle problem identified for palliative care in rural South Dakota. Palliative care is poorly understood by providers and recipients of care. Service reach is also tempered by lack of resources and payer reimbursement constraints. A model for palliative care in these rural communities requires concerted attention to their unique needs and design of services suited for the rural residents.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm mục đích khám phá nhận thức của chuyên gia y tế, bệnh nhân, gia đình và người chăm sóc về chăm sóc giảm nhẹ, tính khả dụng của các dịch vụ chăm sóc giảm nhẹ cho bệnh nhân trên khắp Nam Dakota và tính nhất quán và chất lượng của việc cung cấp dịch vụ chăm sóc giảm nhẹ. PHƯƠNG PHÁP Sáu nhóm tập trung đã được tiến hành trong hai tháng. Những người tham gia bao gồm các thành viên của nhóm chăm sóc sức khỏe liên ngành, bệnh nhân, thành viên gia đình của bệnh nhân và người chăm sóc. Những cá nhân có kinh nghiệm chăm sóc giảm nhẹ hoặc quan tâm đến chăm sóc giảm nhẹ được mời tham gia. Các chiến lược tuyển dụng bao gồm email, tờ rơi và liên hệ trực tiếp bởi các thành viên của Mạng lưới. Lấy mẫu theo kiểu lăn cầu tuyết đã được sử dụng để tuyển dụng những người tham gia. KẾT QUẢ Bốn mươi sáu người tham gia bao gồm bệnh nhân, thành viên gia đình, người chăm sóc và các thành viên trong nhóm chăm sóc sức khỏe liên ngành. Hầu hết những người tham gia là người da trắng (93,3%) và là nữ (80%). Sáu chủ đề chính đã xuất hiện: Nhu cầu hướng dẫn hướng tới việc phát triển mô hình chăm sóc giảm nhẹ toàn tiểu bang toàn diện; Hiểu biết và nhận thức khái niệm kém; Thiếu nguồn lực để triển khai chăm sóc toàn diện tại tất cả các cộng đồng ở Nam Dakota; Sự chênh lệch trong việc cung cấp và cung cấp các dịch vụ chăm sóc tại các cộng đồng SD ở nông thôn; Nhu cầu về mối quan hệ và kết nối với nhóm chăm sóc giảm nhẹ; và Các tác động thứ cấp của chăm sóc giảm nhẹ đối với bệnh nhân / gia đình / người chăm sóc và các thành viên trong nhóm chăm sóc sức khỏe liên ngành. Tầm quan trọng của kết quả: Việc tiếp cận không cân xứng là một vấn đề chính được xác định đối với chăm sóc giảm nhẹ ở vùng nông thôn Nam Dakota. Chăm sóc giảm nhẹ được các nhà cung cấp và người nhận chăm sóc hiểu kém. Phạm vi dịch vụ cũng bị ảnh hưởng bởi sự thiếu hụt nguồn lực và các ràng buộc về hoàn trả của bên chi trả. Một mô hình chăm sóc giảm nhẹ tại các cộng đồng nông thôn này đòi hỏi phải chú ý phối hợp đến các nhu cầu riêng biệt của họ và thiết kế các dịch vụ phù hợp với cư dân nông thôn."} +{"idx": 1870127, "en": "We evaluated whether food insecurity is associated with clinical evidence of diet-sensitive cardiovascular diseases (CVD) risk factors, including obesity, hypertension, dyslipidemia, and diabetes. In this population-based cross-sectional study, 630 women aged 18-50 years under cover of health centers in Iran randomly selected and interviewed face-to-face. Food insecurity was evaluated using a household food insecurity access scale (HFIAS) questionnaire, which its validity and reliability was determined in the Iranian population. Logistic regression was used to assess the associations between food insecurity and having CVD risk factors including general and abdominal obesity, hypertension, hypercholesterolemia, high low-density lipoprotein cholesterol (LDL), low high-density lipoprotein cholesterol (HDL), hypertriglyceridemia, and diabetes. The probability of having hypertriglyceridemia (Adjusted OR = 2.04, P = .031), general obesity (Adjusted OR = 2.43, P = .029), abdominal obesity (Adjusted OR = 2.09, P = .024), and hypertension (Adjusted OR = 2.04, P = .038) increased with exacerbation of women's food insecurity condition. The odds ratio of having CVD risk factors became stronger after adjusting for confounders. Food insecurity was associated with increased risk of CVD risk factors among Iranian reproductive-age women. Efforts to improve food security conditions among women might be associated with reductions in the incidence of CVD-related metabolic risks.", "gemini": "Chúng tôi đã đánh giá liệu tình trạng mất an ninh lương thực có liên quan đến bằng chứng lâm sàng về các yếu tố nguy cơ mắc bệnh tim mạch (CVD) nhạy cảm với chế độ ăn uống, bao gồm béo phì, tăng huyết áp, rối loạn lipid máu và tiểu đường hay không. Trong nghiên cứu cắt ngang dựa trên dân số này, 630 phụ nữ từ 18-50 tuổi được che giấu danh tính tại các trung tâm y tế ở Iran đã được chọn ngẫu nhiên và phỏng vấn trực tiếp. Mất an ninh lương thực được đánh giá bằng bảng câu hỏi thang tiếp cận mất an ninh lương thực hộ gia đình (HFIAS), trong đó tính hợp lệ và độ tin cậy của bảng câu hỏi đã được xác định trong quần thể người Iran. Phân tích hồi quy logistic được sử dụng để đánh giá mối liên quan giữa mất an ninh lương thực và các yếu tố nguy cơ mắc CVD bao gồm béo phì nói chung và béo bụng, tăng huyết áp, tăng cholesterol máu, cholesterol lipoprotein tỷ trọng thấp cao (LDL), cholesterol lipoprotein tỷ trọng cao thấp (HDL), tăng triglycerid máu và tiểu đường. Xác suất mắc tăng triglycerid máu (OR đã hiệu chỉnh = 2,04, P = .031), béo phì nói chung (OR đã hiệu chỉnh = 2,43, P = .029), béo bụng (OR đã hiệu chỉnh = 2,09, P = .024) và tăng huyết áp (OR đã hiệu chỉnh = 2,04, P = .038) tăng lên khi tình trạng mất an ninh lương thực của phụ nữ trầm trọng hơn. Tỷ lệ cược mắc các yếu tố nguy cơ mắc CVD trở nên mạnh hơn sau khi điều chỉnh các yếu tố gây nhiễu. Mất an ninh lương thực có liên quan đến nguy cơ mắc các yếu tố nguy cơ mắc CVD tăng lên ở phụ nữ Iran trong độ tuổi sinh sản. Những nỗ lực cải thiện tình trạng an ninh lương thực ở phụ nữ có thể liên quan đến việc giảm tỷ lệ mắc các nguy cơ chuyển hóa liên quan đến CVD."} +{"idx": 1870128, "en": "Many healthcare organizations have adopted the quadruple aim to create system-level improvements for delivering enhanced experience and outcomes to patients, healthier populations, reduced per-capita costs, and better provider experiences. With a maturing health technology sector, virtual care is gradually being adopted in Canada and proving to be a viable tactic for achieving the quadruple aim. Despite increased acceptance of virtual innovations and their related benefits to patients and providers, implementation of virtual care can be challenging in a Canadian healthcare system. The Ottawa Hospital developed an innovation strategy to guide the adoption and maturity of virtual care as a means of supporting the pursuit of the quadruple aim and achievement of the organization's mission and vision. A case example presenting the strategy and recommendations for health leaders and providers considering implementation of virtual care is discussed.", "gemini": "Nhiều tổ chức chăm sóc sức khỏe đã áp dụng mục tiêu gấp bốn lần để tạo ra những cải tiến ở cấp độ hệ thống nhằm mang lại trải nghiệm và kết quả tốt hơn cho bệnh nhân, quần thể khỏe mạnh hơn, giảm chi phí bình quân đầu người và cải thiện trải nghiệm của nhà cung cấp. Với sự phát triển của lĩnh vực công nghệ y tế, chăm sóc ảo đang dần được áp dụng ở Canada và chứng tỏ là một chiến thuật khả thi để đạt được mục tiêu gấp bốn lần. Mặc dù sự chấp nhận ngày càng tăng đối với các sáng kiến ảo và những lợi ích liên quan của chúng đối với bệnh nhân và nhà cung cấp, nhưng việc triển khai chăm sóc ảo có thể là một thách thức trong hệ thống chăm sóc sức khỏe của Canada. Bệnh viện Ottawa đã phát triển một chiến lược đổi mới để hướng dẫn việc áp dụng và trưởng thành của chăm sóc ảo như một phương tiện hỗ trợ việc theo đuổi mục tiêu gấp bốn lần và đạt được sứ mệnh và tầm nhìn của tổ chức. Một ví dụ về trường hợp trình bày chiến lược và các khuyến nghị cho các nhà lãnh đạo và nhà cung cấp dịch vụ y tế đang cân nhắc triển khai dịch vụ chăm sóc ảo được thảo luận."} +{"idx": 1870129, "en": "The recent COVID-19 pandemic has highlighted limitations in current healthcare systems and needed strategies to increase surgical access. This article presents a team-based integration model that embraces intra-disciplinary collaboration in shared clinical care, professional development, and administrative processes to address this surge in demand for surgical care. Implementing this model will require communicating the rationale for and benefits of shared care, while shifting patient trust to a team of providers. For the individual surgeon, advantages of clinical integration through shared care include decreased burnout and professional isolation, and more efficient transitions into and out of practice. Advantages to the system include greater surgeon availability, streamlined disease site wait lists, and promotion of system efficiency through a centralized distribution of clinical resources. We present a framework to stimulate national dialogue around shared care that will ultimately help overcome system bottlenecks for surgical patients and provide support for health professionals.", "gemini": "Đại dịch COVID-19 gần đây đã làm nổi bật những hạn chế trong hệ thống chăm sóc sức khỏe hiện tại và các chiến lược cần thiết để tăng khả năng tiếp cận phẫu thuật. Bài viết này trình bày một mô hình tích hợp theo nhóm, bao gồm sự hợp tác giữa các chuyên ngành trong chăm sóc lâm sàng chung, phát triển chuyên môn và các quy trình hành chính để giải quyết sự gia tăng nhu cầu về chăm sóc phẫu thuật này. Việc triển khai mô hình này sẽ đòi hỏi phải truyền đạt lý do và lợi ích của việc chăm sóc chung, đồng thời chuyển sự tin tưởng của bệnh nhân sang một nhóm các nhà cung cấp dịch vụ. Đối với từng bác sĩ phẫu thuật, những lợi thế của việc tích hợp lâm sàng thông qua chăm sóc chung bao gồm giảm tình trạng kiệt sức và cô lập nghề nghiệp, cũng như quá trình chuyển đổi hiệu quả hơn khi vào và ra khỏi phòng khám. Những lợi thế đối với hệ thống bao gồm khả năng tiếp cận bác sĩ phẫu thuật tốt hơn, danh sách chờ tại địa điểm bệnh được sắp xếp hợp lý và thúc đẩy hiệu quả của hệ thống thông qua việc phân phối tập trung các nguồn lực lâm sàng. Chúng tôi trình bày một khuôn khổ để kích thích đối thoại quốc gia xung quanh việc chăm sóc chung, điều này cuối cùng sẽ giúp khắc phục tình trạng tắc nghẽn của hệ thống đối với bệnh nhân phẫu thuật và hỗ trợ các chuyên gia y tế."} +{"idx": 1870130, "en": "In Quebec, the strategic leadership of nursing directorates remains poorly documented despite its importance for the performance of their organizations. Using three focus groups and 31 individual semistructured interviews, a qualitative descriptive study was conducted, including 35 participants from 18 of the Quebec 34 health institutions created in 2015 by the last reform. Seven themes emerged: (1) taking ownership of the strategic positioning, (2) developing and communicating a vision, (3) making strategic, systematic, and measured choices, (4) reframing roles, (5) getting involved in the strategic decision-making processes, (6) developing the political capacity, and (7) building alliances. Four professional and organizational components influenced the nursing directorates' leadership capacity: clinical credibility, a sufficient number of people educated at the graduate level, organizational culture, and size of the institution. It is expected that these results regarding nursing directorates' exercise of strategic leadership will lead to better governance and quality of nursing care.", "gemini": "Tại Quebec, vai trò lãnh đạo chiến lược của các giám đốc điều dưỡng vẫn chưa được ghi chép đầy đủ mặc dù vai trò này rất quan trọng đối với hiệu suất của các tổ chức. Sử dụng ba nhóm tập trung và 31 cuộc phỏng vấn bán cấu trúc riêng lẻ, một nghiên cứu mô tả định tính đã được tiến hành, bao gồm 35 người tham gia từ 18 trong số 34 cơ sở y tế của Quebec được thành lập vào năm 2015 theo cải cách gần đây nhất. Bảy chủ đề đã xuất hiện: (1) tiếp quản vị trí chiến lược, (2) phát triển và truyền đạt tầm nhìn, (3) đưa ra các lựa chọn có tính chiến lược, có hệ thống và có cân nhắc, (4) định hình lại vai trò, (5) tham gia vào các quy trình ra quyết định chiến lược, (6) phát triển năng lực chính trị và (7) xây dựng liên minh. Bốn thành phần chuyên môn và tổ chức đã ảnh hưởng đến năng lực lãnh đạo của các giám đốc điều dưỡng: uy tín lâm sàng, số lượng người được đào tạo ở trình độ sau đại học, văn hóa tổ chức và quy mô của tổ chức. Dự kiến rằng những kết quả này liên quan đến việc các giám đốc điều dưỡng thực hiện vai trò lãnh đạo chiến lược sẽ dẫn đến sự quản trị và chất lượng chăm sóc điều dưỡng tốt hơn."} +{"idx": 1870131, "en": "Rhythm research has had a long tradition in psychiatry, especially in affective disorders. The study of trends in incidence plays a central role in epidemiology and public health. The aims of this research were to describe the socio-demographic and clinical characteristics of persons admitted for psychiatric hospitalization and their trends and periodicity in cases (global and by groups) in Spain over the 11 year study span. We conducted a cross-sectional study of the hospital discharge database of Castilla y León from 2005 to 2015, selecting hospitalizations for psychiatric reasons. Trends in the rates of hospitalization were studied by joinpoint regression analysis. Time series analysis for periodicities was done by spectral analysis, fast Fourier transform, and cosinor analysis. Some 49501 hospitalizations due to psychiatric disorders, out of 2717192 hospital admissions, took place during the study span. Hospitalizations for psychosis were frequent (15949, 32.2%), while such for eating disorders were infrequent, but showed the highest average stay (28 days) and DRG relative weight (2.41). The general trend was a statistically significant 2% annual increase in psychiatric hospitalizations over the 11 year span; substance abuse was the only exception to this trend. The whole population and the subgroups of psychosis and bipolar disorders showed significant circannual (one-year) variation in admissions. The rhythm percentage of the global group was 11.4%, while the rhythm percentages of the psychosis, bipolar, and eating disorders were 17.1%, 17.5%, and 9.6%, respectively (p < .05).", "gemini": "Nghiên cứu về nhịp điệu đã có một truyền thống lâu đời trong ngành tâm thần học, đặc biệt là trong các rối loạn cảm xúc. Nghiên cứu về xu hướng mắc bệnh đóng vai trò trung tâm trong dịch tễ học và sức khỏe cộng đồng. Mục đích của nghiên cứu này là mô tả các đặc điểm về mặt xã hội-dân số và lâm sàng của những người được nhập viện điều trị tâm thần và xu hướng cũng như chu kỳ của họ trong các trường hợp (toàn cầu và theo nhóm) tại Tây Ban Nha trong khoảng thời gian nghiên cứu là 11 năm. Chúng tôi đã tiến hành một nghiên cứu cắt ngang về cơ sở dữ liệu xuất viện của Castilla y León từ năm 2005 đến năm 2015, lựa chọn các trường hợp nhập viện vì lý do tâm thần. Các xu hướng về tỷ lệ nhập viện đã được nghiên cứu bằng phân tích hồi quy điểm nối. Phân tích chuỗi thời gian về chu kỳ đã được thực hiện bằng phân tích phổ, biến đổi Fourier nhanh và phân tích cosinor. Khoảng 49501 trường hợp nhập viện do rối loạn tâm thần, trong số 2717192 trường hợp nhập viện, đã diễn ra trong khoảng thời gian nghiên cứu. Nhập viện vì bệnh loạn thần thường gặp (15949, 32,2%), trong khi nhập viện vì rối loạn ăn uống không thường gặp, nhưng có thời gian nằm viện trung bình cao nhất (28 ngày) và trọng số DRG tương đối (2,41). Xu hướng chung là tỷ lệ nhập viện tâm thần tăng 2% hàng năm có ý nghĩa thống kê trong khoảng thời gian 11 năm; lạm dụng chất gây nghiện là trường hợp ngoại lệ duy nhất đối với xu hướng này. Toàn bộ dân số và các nhóm phụ loạn thần và rối loạn lưỡng cực đều có sự thay đổi theo chu kỳ hàng năm (một năm) đáng kể trong các lần nhập viện. Tỷ lệ nhịp điệu của nhóm toàn cầu là 11,4%, trong khi tỷ lệ nhịp điệu của các nhóm loạn thần, lưỡng cực và rối loạn ăn uống lần lượt là 17,1%, 17,5% và 9,6% (p < 0,05)."} +{"idx": 1870132, "en": "Cyclophosphamide (CP) is widely used as a chemotherapy against various types of cancers. However, CP is accompanied with multiple organ toxicity due to production of reactive oxygen species (ROS), induction of inflammation and consequently apoptosis. Alogliptin (Alo) is a dipeptidyl peptidase 4 (DPP-IV) inhibitor, which is booming as an antidiabetic agent. Interestingly, gliptins are currently studied for their counter-regulatory effects against oxidative stress and inflammation via multiple pathways, among which is the mitogen-activated protein kinase (MAPK)/c-Jun N-terminal kinase (JNK) pathway. This cascade can reduce inflammation via mitigating the activity of mothers against decapentaplegic homolog 3 (SMAD3) and c-Jun. However, Alo effect against CP-induced kidney injury has not been previously elucidated. This tempted us to investigate the possible beneficial effect of Alo against CP-induced kidney injury via modulating the MAP3K/JNK/SMAD3 signaling cascade. Thirty-two male Wistar rats were randomly allocated into four groups. CP-treated group received a single dose of CP (200 mg/kg; i.p.). Alo-treated group received Alo (20 mg/kg/day; p.o.) for 7 days with single CP injection on day 2. Marked decrease in renal injury was observed upon Alo treatment, as evidenced through declined serum kidney function markers, oxidative stress and apoptosis markers, MAP3K expression, phospho (p)-SMAD3, p-JNK, and p-c-Jun levels. These cellular effects were reflected in reduced transforming growth factor beta (TGF-β) and tumor necrosis factor alpha (TNF-α) fibrotic and inflammatory mediators, coinciding with improved histopathological portrait. In conclusion, the current study provides novel application of Alo as a therapeutic modality against CP-induced nephrotoxicity.", "gemini": "Cyclophosphamide (CP) được sử dụng rộng rãi như một loại hóa trị liệu chống lại nhiều loại ung thư khác nhau. Tuy nhiên, CP đi kèm với tình trạng nhiễm độc nhiều cơ quan do sản xuất các loài oxy phản ứng (ROS), gây viêm và do đó gây apoptosis. Alogliptin (Alo) là chất ức chế dipeptidyl peptidase 4 (DPP-IV), đang phát triển mạnh như một tác nhân chống đái tháo đường. Điều thú vị là, gliptin hiện đang được nghiên cứu về tác dụng điều hòa ngược của chúng đối với stress oxy hóa và tình trạng viêm thông qua nhiều con đường, trong đó có con đường mitogen-activated protein kinase (MAPK)/c-Jun N-terminal kinase (JNK). Quá trình này có thể làm giảm tình trạng viêm thông qua việc giảm hoạt động của các bà mẹ chống lại đồng đẳng decapentaplegic 3 (SMAD3) và c-Jun. Tuy nhiên, tác dụng của Alo đối với tổn thương thận do CP gây ra trước đây chưa được làm sáng tỏ. Điều này đã thôi thúc chúng tôi nghiên cứu tác dụng có lợi có thể có của Alo đối với tổn thương thận do CP gây ra thông qua việc điều chỉnh chuỗi tín hiệu MAP3K/JNK/SMAD3. Ba mươi hai con chuột Wistar đực được phân bổ ngẫu nhiên vào bốn nhóm. Nhóm được điều trị bằng CP nhận được một liều duy nhất CP (200 mg/kg; i.p.). Nhóm được điều trị bằng Alo nhận được Alo (20 mg/kg/ngày; p.o.) trong 7 ngày với một lần tiêm CP vào ngày thứ 2. Giảm đáng kể tổn thương thận được quan sát thấy khi điều trị bằng Alo, bằng chứng là giảm các dấu hiệu chức năng thận trong huyết thanh, các dấu hiệu stress oxy hóa và apoptosis, biểu hiện MAP3K, phospho (p)-SMAD3, p-JNK và p-c-Jun. Các tác dụng của tế bào này được phản ánh trong việc giảm yếu tố tăng trưởng chuyển đổi beta (TGF-β) và các chất trung gian xơ hóa và viêm của yếu tố hoại tử khối u alpha (TNF-α), trùng hợp với hình ảnh mô bệnh học được cải thiện. Tóm lại, nghiên cứu hiện tại cung cấp ứng dụng mới của Alo như một phương thức điều trị chống lại độc tính thận do CP gây ra."} +{"idx": 1870133, "en": "OBJECTIVES Anemia is often seen in chronic obstructive pulmonary disease (COPD) patients. However, the implications of anemia on the outcomes of patients with COPD is not clearly known. Therefore, we conducted a systematic review and meta-analysis to evaluate the effects of anemia on the morbidity and mortality of patients with COPD. METHODS We followed PRISMA guidelines to perform a systematic identification of relevant publications in the literature on four academic databases: MEDLINE, Scopus, EMBASE, and CENTRAL. RESULTS Out of 1845 records, we included data from 15 articles including 420 970 participants in this review. Our systematic review presents a 2b level of evidence suggesting a higher duration of hospital stay, Charlson comorbidity index score, and mortality rate for patients with COPD and anemia than for patients with COPD without anemia. The meta-analysis revealed a moderate effect increase in the mean duration of hospital stay (Hedge's g, 0.55), Charlson comorbidity index (0.68), and mortality rate (0.49) in patients with COPD and anemia as compared to those in patients with COPD without anemia. CONCLUSION The current systematic review and meta-analysis provide evidence regarding the detrimental consequences of anemia on the morbidity and mortality of patients with COPD.", "gemini": "MỤC TIÊU Thiếu máu thường gặp ở những bệnh nhân mắc bệnh phổi tắc nghẽn mạn tính (COPD). Tuy nhiên, những ảnh hưởng của thiếu máu đến kết cục của những bệnh nhân mắc COPD vẫn chưa được biết rõ. Do đó, chúng tôi đã tiến hành một đánh giá có hệ thống và phân tích tổng hợp để đánh giá tác động của thiếu máu đến tỷ lệ mắc bệnh và tử vong của những bệnh nhân mắc COPD. PHƯƠNG PHÁP Chúng tôi tuân theo các hướng dẫn của PRISMA để thực hiện xác định có hệ thống các ấn phẩm có liên quan trong tài liệu trên bốn cơ sở dữ liệu học thuật: MEDLINE, Scopus, EMBASE và CENTRAL. KẾT QUẢ Trong số 1845 hồ sơ, chúng tôi đã đưa dữ liệu từ 15 bài báo bao gồm 420.970 người tham gia vào đánh giá này. Đánh giá có hệ thống của chúng tôi đưa ra mức độ bằng chứng 2b cho thấy thời gian nằm viện, điểm chỉ số bệnh đi kèm Charlson và tỷ lệ tử vong cao hơn ở những bệnh nhân mắc COPD và thiếu máu so với những bệnh nhân mắc COPD không thiếu máu. Phân tích tổng hợp cho thấy mức độ tác dụng vừa phải làm tăng thời gian nằm viện trung bình (g của Hedge, 0,55), chỉ số bệnh đi kèm Charlson (0,68) và tỷ lệ tử vong (0,49) ở những bệnh nhân mắc COPD và thiếu máu so với những bệnh nhân mắc COPD không thiếu máu. KẾT LUẬN Đánh giá có hệ thống và phân tích tổng hợp hiện tại cung cấp bằng chứng về những hậu quả bất lợi của thiếu máu đối với tỷ lệ mắc bệnh và tử vong của những bệnh nhân mắc COPD."} +{"idx": 1870134, "en": "INTRODUCTION Triple negative breast cancer (TNBC) was once thought to be an insurmountable disease marked by a lack of targeted treatments. However, we are now witnessing the dawn of targeted therapies for TNBC in which progress has stemmed from an improved understanding of the components that make TNBC unique. The identification of biomarkers, such as BRCA1/2, PIK3CA and RSK2, have advanced the field remarkably and there is considerable interest in finding novel therapeutics for TNBC that offer durable clinical benefit with fewer adverse events. AREAS COVERED We discuss phase I/II trials of new and emerging targeted therapies for TNBC, according to ClinicalTrials.gov up to June 2020. Although the emphasis is on ongoing and completed early phase trials, we also highlight pivotal studies that have led to the approval of new targeted classes of drugs for TNBC, with a focus on outcomes and common adverse events of each class of therapy. EXPERT OPINION The way forward for TNBC treatment is through precision medicine. The use of novel agents matched with biomarkers to identify patients with the best chance of sustainable response offers new hope. We now have great potential for improving the outcomes for patients with TNBC.", "gemini": "GIỚI THIỆU Ung thư vú ba âm tính (TNBC) từng được coi là một căn bệnh không thể vượt qua, không có các phương pháp điều trị đích. Tuy nhiên, hiện nay chúng ta đang chứng kiến sự ra đời của các liệu pháp điều trị đích cho TNBC, trong đó tiến bộ bắt nguồn từ sự hiểu biết được cải thiện về các thành phần tạo nên sự độc đáo của TNBC. Việc xác định các dấu ấn sinh học, chẳng hạn như BRCA1/2, PIK3CA và RSK2, đã thúc đẩy lĩnh vực này phát triển đáng kể và có sự quan tâm đáng kể trong việc tìm ra các liệu pháp điều trị mới cho TNBC mang lại lợi ích lâm sàng lâu dài với ít tác dụng phụ hơn. CÁC LĨNH VỰC ĐƯỢC BAO PHỦ Chúng tôi thảo luận về các thử nghiệm giai đoạn I/II của các liệu pháp điều trị đích mới và đang nổi lên cho TNBC, theo ClinicalTrials.gov cho đến tháng 6 năm 2020. Mặc dù trọng tâm là các thử nghiệm giai đoạn đầu đang diễn ra và đã hoàn thành, chúng tôi cũng nêu bật các nghiên cứu quan trọng đã dẫn đến việc chấp thuận các loại thuốc đích mới cho TNBC, tập trung vào kết quả và các tác dụng phụ phổ biến của từng loại liệu pháp. Ý KIẾN CỦA CHUYÊN GIA Con đường phía trước cho điều trị TNBC là thông qua y học chính xác. Việc sử dụng các tác nhân mới kết hợp với các dấu ấn sinh học để xác định những bệnh nhân có cơ hội đáp ứng bền vững mang lại hy vọng mới. Hiện nay, chúng ta có tiềm năng lớn để cải thiện kết quả cho những bệnh nhân mắc TNBC."} +{"idx": 1870135, "en": "INTRODUCTION Precision medicine has revolutionized the diagnostic and therapeutic management of acute myeloid leukemia (AML), from standardized schemes based on chemotherapy to tailored approaches according to molecular and genetic profile and targeted therapy. AREAS COVERED The main topics of precision medicine in AML were reviewed in MEDLINE, EMBASE, and Cochrane Central Register databases, and future directions in this therapeutic area were addressed. This review included targeted therapies, drug-sensitivity tests and predictive biomarkers, and genetic studies employing pharmacogenetic and deep sequencing strategies. EXPERT OPINION Precision medicine has opened the door to personalized therapy for specific AML patient populations with promising results. Several targeted therapies have been approved or are being tested for specific mutations (i.e. FLT3, IDH, BCL-2, TP53), obtaining improvements in clinical outcomes and less toxicity as compared with intensive treatment, allowing potential combination therapy. Ongoing trials and real data will establish the role of these molecules in monotherapy or combined in different AML settings (front-line, relapsed/refractory, or post-transplant). Experience in drug-sensitivity predictors and pharmacogenetic biomarkers is encouraging and could be useful tools in the next years, but we need a better understanding of AML biology and pathogenesis as well as confirmatory studies to demonstrate the utility in clinical practice.", "gemini": "Y HỌC CHÍNH XÁC Y học chính xác đã cách mạng hóa việc quản lý chẩn đoán và điều trị bệnh bạch cầu cấp dòng tủy (AML), từ các phác đồ chuẩn hóa dựa trên hóa trị liệu đến các phương pháp tiếp cận được điều chỉnh theo hồ sơ phân tử và di truyền và liệu pháp nhắm trúng đích. CÁC LĨNH VỰC ĐƯỢC BAO PHỦ Các chủ đề chính của y học chính xác trong AML đã được xem xét trong các cơ sở dữ liệu MEDLINE, EMBASE và Cochrane Central Register, và các hướng đi trong tương lai trong lĩnh vực điều trị này đã được đề cập. Đánh giá này bao gồm các liệu pháp nhắm trúng đích, xét nghiệm độ nhạy thuốc và các dấu ấn sinh học dự đoán, và các nghiên cứu di truyền sử dụng các chiến lược dược di truyền và giải trình tự sâu. Ý KIẾN CỦA CHUYÊN GIA Y học chính xác đã mở ra cánh cửa cho liệu pháp cá nhân hóa cho các quần thể bệnh nhân AML cụ thể với kết quả đầy hứa hẹn. Một số liệu pháp nhắm trúng đích đã được chấp thuận hoặc đang được thử nghiệm cho các đột biến cụ thể (tức là FLT3, IDH, BCL-2, TP53), đạt được cải thiện về kết quả lâm sàng và ít độc tính hơn so với điều trị chuyên sâu, cho phép kết hợp liệu pháp tiềm năng. Các thử nghiệm đang diễn ra và dữ liệu thực sẽ thiết lập vai trò của các phân tử này trong liệu pháp đơn trị hoặc kết hợp trong các điều kiện AML khác nhau (tuyến đầu, tái phát/kháng thuốc hoặc sau ghép). Kinh nghiệm về các yếu tố dự đoán độ nhạy thuốc và các dấu ấn sinh học dược di truyền rất đáng khích lệ và có thể là những công cụ hữu ích trong những năm tới, nhưng chúng ta cần hiểu rõ hơn về sinh học và bệnh sinh của AML cũng như các nghiên cứu xác nhận để chứng minh tính hữu ích trong thực hành lâm sàng."} +{"idx": 1870136, "en": "β-Thalassemia (β-thal) is one of the most common diseases in Iran. Here, we report the spectrum of HBB gene mutations in 176 Kurdish β-thal carriers from the northern part of Ilam Province, Iran. The amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used to identify common β-globin gene mutations observed in Iran. Samples negative on ARMS-PCR were analyzed by direct sequencing of the β-globin gene. In total, 12 different mutations were identified on the β-globin gene. The mutations of IVS-II-1 (G>A) (HBB: c.315+1G>A), codons 8/9 (+G) (HBB: c.27_28insG), codons 36/37 (-T) (HBB: c.112delT) and IVS-I-110 (G>A) (HBB: c.93-21G>A), were the most prevalent mutations in our samples, with frequencies of 59.09, 10.80, 7.95 and 7.39%, respectively. In general, the mutation spectrum of the β-globin gene in the northern part of Ilam Province is most similar to that in other western provinces of Iran. On the other hand, due to the high prevalence of carriers and β-thal major (β-TM) patients in this province, our results can be helpful in identifying carriers as well as at-risk fetuses through the prenatal diagnosis program.", "gemini": "β-Thalassemia (β-thal) là một trong những bệnh phổ biến nhất ở Iran. Ở đây, chúng tôi báo cáo phổ đột biến gen HBB ở 176 người mang β-thal người Kurd từ phía bắc tỉnh Ilam, Iran. Kỹ thuật phản ứng khuếch đại đột biến - phản ứng chuỗi polymerase (ARMS-PCR) đã được sử dụng để xác định các đột biến gen β-globin phổ biến được quan sát thấy ở Iran. Các mẫu âm tính trên ARMS-PCR được phân tích bằng cách giải trình tự trực tiếp gen β-globin. Tổng cộng, 12 đột biến khác nhau đã được xác định trên gen β-globin. Các đột biến của IVS-II-1 (G>A) (HBB: c.315+1G>A), codon 8/9 (+G) (HBB: c.27_28insG), codon 36/37 (-T) (HBB: c.112delT) và IVS-I-110 (G>A) (HBB: c.93-21G>A), là những đột biến phổ biến nhất trong các mẫu của chúng tôi, với tần số lần lượt là 59,09, 10,80, 7,95 và 7,39%. Nói chung, phổ đột biến của gen β-globin ở phía bắc tỉnh Ilam tương tự nhất với phổ đột biến ở các tỉnh phía tây khác của Iran. Mặt khác, do tỷ lệ người mang và bệnh nhân β-thal thể nặng (β-TM) cao ở tỉnh này, kết quả của chúng tôi có thể hữu ích trong việc xác định người mang cũng như thai nhi có nguy cơ thông qua chương trình chẩn đoán trước sinh."} +{"idx": 1870137, "en": "INTRODUCTION Recent years have seen a tremendous increase in the availability of therapeutic options for the management of patients with chronic lymphocytic leukemia (CLL). Notable among those are Bruton's tyrosine kinase (BTK) and b-cell lymphoma-2 (bcl-2) inhibitors. AREAS COVERED The authors provide a brief overview of the BTK signaling pathway as well as available, approved BTK inhibitors for CLL. In addition, they review pre-clinical and clinical data related to zanubrutinib and its use and CLL and other lymphoid malignancies. EXPERT OPINION Two BTK inhibitors are currently Food and Drug Administration (FDA) approved for use in CLL, and multiple additional agents are in development. Zanubrutinib is currently approved for the treatment of patients with relapsed mantle cell lymphoma and has demonstrated an impressive safety and efficacy profile. The choice of a specific BTK inhibitor for clinical use is dependent on its efficacy and relative toxicity profile. In addition, drug interactions also influence this decision. Zanubrutinib therefore provides an exciting option to utilize a specific BTK inhibitor with potentially limited toxicities. Additional comparative studies are currently underway to establish its advantage over currently available BTK inhibitors. Combination strategies are also being pursued to increase the depth and durability of remissions.", "gemini": "GIỚI THIỆU Những năm gần đây đã chứng kiến sự gia tăng đáng kể về các lựa chọn điều trị cho việc quản lý bệnh nhân bị bệnh bạch cầu lymphocytic mãn tính (CLL). Đáng chú ý trong số đó là các chất ức chế kinase tyrosine Bruton (BTK) và b-cell lymphoma-2 (bcl-2). CÁC LĨNH VỰC ĐƯỢC BAO PHỦ Các tác giả cung cấp một cái nhìn tổng quan ngắn gọn về con đường truyền tín hiệu BTK cũng như các chất ức chế BTK đã được chấp thuận, có sẵn cho CLL. Ngoài ra, họ xem xét dữ liệu tiền lâm sàng và lâm sàng liên quan đến zanubrutinib và việc sử dụng của nó và CLL và các bệnh ác tính khác của hệ bạch huyết. Ý KIẾN CỦA CHUYÊN GIA Hai chất ức chế BTK hiện được Cục Quản lý Thực phẩm và Dược phẩm (FDA) chấp thuận sử dụng trong CLL và nhiều tác nhân bổ sung khác đang được phát triển. Zanubrutinib hiện được chấp thuận để điều trị bệnh nhân u lympho tế bào áo choàng tái phát và đã chứng minh được hồ sơ an toàn và hiệu quả ấn tượng. Việc lựa chọn một chất ức chế BTK cụ thể để sử dụng lâm sàng phụ thuộc vào hiệu quả và hồ sơ độc tính tương đối của nó. Ngoài ra, tương tác thuốc cũng ảnh hưởng đến quyết định này. Do đó, Zanubrutinib cung cấp một lựa chọn thú vị để sử dụng một chất ức chế BTK cụ thể với khả năng hạn chế độc tính. Các nghiên cứu so sánh bổ sung hiện đang được tiến hành để thiết lập lợi thế của nó so với các chất ức chế BTK hiện có. Các chiến lược kết hợp cũng đang được theo đuổi để tăng độ sâu và độ bền của tình trạng thuyên giảm."} +{"idx": 1870138, "en": "INTRODUCTION Nonergot dopamine agonists (NEDA) represent an excellent treatment option for Parkinson's disease (PD) patients, in both early and advanced stages of the disease. The post-marketing phase of NEDA has highlighted, though, the occurrence of important long-term adverse events. AREAS COVERED This review reports recent updates on NEDA adverse events, analyzing neurobiological bases and risk factors of these complications. A literature search has been performed using Medline and reviewing the bibliographies of selected articles. EXPERT OPINION NEDA represents a very important option in the treatment of PD. Criticisms on their use can be overcome through a better knowledge of these molecules and of the risk factors for adverse events which allow specialists to prevent the occurrence of undesired complications and consent a tailor-based approach. Abbreviations: PD: Parkinson's disease, DA: dopamine agonists, NEDA: non-ergot dopamine agonists, ICD: impulse control disorders, DAWS: dopamine agonist withdrawal syndrome, CYP: Cytochrome P, PK: pharmacokinetic, AUC: area under the curve, HRT: hormone replacement therapy, AV: atrioventricular, HF: heart failure, OH: orthostatic hypotension, RBD: REM behavior disorders, PDP: Parkinson's disease psychosis, DRT: dopamine replacement therapy, DDS: dopamine dysregulation syndrome, MMSE: Mini-Mental state examination, EDS: excessive daytime somnolence.", "gemini": "GIỚI THIỆU Các chất chủ vận dopamine không phải ergot (NEDA) là một lựa chọn điều trị tuyệt vời cho bệnh nhân mắc bệnh Parkinson (PD), ở cả giai đoạn đầu và giai đoạn tiến triển của bệnh. Tuy nhiên, giai đoạn sau khi đưa ra thị trường của NEDA đã làm nổi bật sự xuất hiện của các tác dụng phụ quan trọng trong thời gian dài. CÁC LĨNH VỰC ĐƯỢC BAO TRÙM Đánh giá này báo cáo các thông tin cập nhật gần đây về các tác dụng phụ của NEDA, phân tích các cơ sở thần kinh sinh học và các yếu tố nguy cơ của những biến chứng này. Đã thực hiện tìm kiếm tài liệu bằng cách sử dụng Medline và xem xét các tài liệu tham khảo của các bài báo được chọn. Ý KIẾN CỦA CHUYÊN GIA NEDA là một lựa chọn rất quan trọng trong điều trị bệnh PD. Có thể khắc phục những lời chỉ trích về việc sử dụng chúng thông qua việc hiểu biết tốt hơn về các phân tử này và các yếu tố nguy cơ gây ra tác dụng phụ, cho phép các chuyên gia ngăn ngừa sự xuất hiện của các biến chứng không mong muốn và đồng ý với phương pháp tiếp cận dựa trên từng người. Các chữ viết tắt: PD: bệnh Parkinson, DA: chất chủ vận dopamine, NEDA: chất chủ vận dopamine không phải ergot, ICD: rối loạn kiểm soát xung lực, DAWS: hội chứng cai chất chủ vận dopamine, CYP: Cytochrome P, PK: dược động học, AUC: diện tích dưới đường cong, HRT: liệu pháp thay thế hormone, AV: nhĩ thất, HF: suy tim, OH: hạ huyết áp tư thế đứng, RBD: rối loạn hành vi REM, PDP: loạn thần do bệnh Parkinson, DRT: liệu pháp thay thế dopamine, DDS: hội chứng rối loạn dopamine, MMSE: kiểm tra trạng thái tinh thần nhỏ, EDS: buồn ngủ quá mức vào ban ngày."} +{"idx": 1870139, "en": "BACKGROUND Narrowband UV-B (NBUVB) phototherapy is the mainstay of vitiligo treatment, but hyperpigmentation is one of the limitations. Meanwhile, topical tretinoin is effective against pigmentary disorders. OBJECTIVE To determine whether tretinoin 0.05% cream would prevent hyperpigmentation when patients with facial vitiligo underwent phototherapy. METHODS A randomized, controlled, split-face trial was conducted. Adult patients with stable, non-segmental facial vitiligo were enrolled. The left/right sides of the face were randomly allocated to receive either topical tretinoin 0.05% cream or moisturizer twice daily. The entire face was subjected to NBUVB phototherapy twice weekly for 12 weeks. The degree of hyperpigmentation was assessed as the delta L* (brightness) value of the darkest spot in each side of the face at baseline and every 4 weeks. The degree of repigmentation was assessed. RESULTS Twenty-five patients were enrolled; 21 completed the study. The delta L* value was significantly different between the two groups: -0.5% in the tretinoin group and -8.7% in the control group at 12 weeks (p = .002). Marked repigmentation was achieved in 15 patients of both groups. CONCLUSIONS Tretinoin 0.05% cream prevented hyperpigmentation during NBUVB phototherapy in patients with facial vitiligo, and did not compromise the overall treatment response. TRIAL REGISTRATION ClinicalTrials.gov NCT03933774.", "gemini": "BỐI CẢNH Liệu pháp quang học bằng tia UV-B hẹp (NBUVB) là phương pháp điều trị chính cho bệnh bạch biến, nhưng tăng sắc tố là một trong những hạn chế. Trong khi đó, tretinoin tại chỗ có hiệu quả chống lại các rối loạn sắc tố. MỤC TIÊU Xác định xem kem tretinoin 0,05% có ngăn ngừa được tình trạng tăng sắc tố khi bệnh nhân bị bạch biến ở mặt phải trải qua liệu pháp quang học hay không. PHƯƠNG PHÁP Đã tiến hành thử nghiệm chia mặt, ngẫu nhiên, có đối chứng. Những bệnh nhân trưởng thành bị bạch biến ở mặt không phân đoạn, ổn định đã được ghi danh. Các bên trái/phải của khuôn mặt được phân bổ ngẫu nhiên để bôi kem tretinoin 0,05% tại chỗ hoặc kem dưỡng ẩm hai lần mỗi ngày. Toàn bộ khuôn mặt được chiếu xạ bằng liệu pháp quang học NBUVB hai lần một tuần trong 12 tuần. Mức độ tăng sắc tố được đánh giá là giá trị delta L* (độ sáng) của điểm tối nhất ở mỗi bên mặt lúc ban đầu và cứ sau 4 tuần. Đánh giá mức độ tái sắc tố. KẾT QUẢ Đã ghi danh 25 bệnh nhân; 21 người đã hoàn thành nghiên cứu. Giá trị delta L* khác biệt đáng kể giữa hai nhóm: -0,5% ở nhóm tretinoin và -8,7% ở nhóm đối chứng sau 12 tuần (p = 0,002). Tái sắc tố rõ rệt đạt được ở 15 bệnh nhân của cả hai nhóm. KẾT LUẬN Kem tretinoin 0,05% ngăn ngừa tình trạng tăng sắc tố trong quá trình điều trị bằng liệu pháp quang học NBUVB ở những bệnh nhân bị bạch biến ở mặt và không ảnh hưởng đến đáp ứng điều trị chung. ĐĂNG KÝ THỬ NGHIỆM ClinicalTrials.gov NCT03933774."} +{"idx": 1870140, "en": "Background Cardiovascular risk burden has been linked to cardiovascular disease (CVD) and cognitive decline, but its association with disability is unclear. We aimed to examined the association of cardiovascular risk burden assessed by the Framingham general cardiovascular risk score (FGCRS) with the risk and progression of disability and estimated the extent to which CVD and cognitive decline mediate this association. Methods and Results A total of 1480 older adults with no disabilities (mean age=79.32±7.38 years) from the Rush Memory and Aging Project were followed for up to 21 years. FGCRS at baseline was calculated and categorized into tertiles. Disability was assessed annually with activities of daily living. The number of CVDs was calculated by summing up the CVD events. Global cognitive function was assessed annually with a battery of 19 tests. Data were analyzed using the Cox model, linear mixed effects model, and mediation analysis. At the end of the follow-up, 713 (48.2%) participants developed disability. Compared with the lowest tertile of the FGCRS, the multiadjusted hazards ratios of disability were 1.34 (95% CI, 1.11-1.62) for the highest tertile. In addition, the highest FGCRS was associated with a change in activities of daily living score over time (β=0.057; 95% CI, 0.021-0.093). The association between FGCRS and change in activities of daily living was 13.8% mediated by the accumulation of CVDs and 25.1% by cognitive decline, respectively. Conclusions Higher cardiovascular risk burden increased the risk of disability and accelerated its progression over time. CVD accumulation and cognitive decline may partially mediate the association.", "gemini": "Bối cảnh Gánh nặng nguy cơ tim mạch có liên quan đến bệnh tim mạch (CVD) và suy giảm nhận thức, nhưng mối liên hệ của nó với tình trạng khuyết tật vẫn chưa rõ ràng. Chúng tôi nhằm mục đích kiểm tra mối liên quan của gánh nặng nguy cơ tim mạch được đánh giá bằng điểm số nguy cơ tim mạch chung của Framingham (FGCRS) với nguy cơ và tiến triển của tình trạng khuyết tật và ước tính mức độ mà CVD và suy giảm nhận thức có thể ảnh hưởng đến mối liên quan này. Phương pháp và kết quả Tổng cộng 1480 người lớn tuổi không bị khuyết tật (tuổi trung bình = 79,32 ± 7,38 tuổi) từ Dự án lão hóa và trí nhớ Rush đã được theo dõi trong tối đa 21 năm. FGCRS lúc ban đầu được tính toán và phân loại thành các phần ba. Khuyết tật được đánh giá hàng năm bằng các hoạt động sinh hoạt hàng ngày. Số lượng CVD được tính bằng cách cộng các sự kiện CVD lại với nhau. Chức năng nhận thức toàn cầu được đánh giá hàng năm bằng một loạt 19 bài kiểm tra. Dữ liệu được phân tích bằng mô hình Cox, mô hình hiệu ứng hỗn hợp tuyến tính và phân tích trung gian. Vào cuối thời gian theo dõi, 713 (48,2%) người tham gia đã bị khuyết tật. So với phần ba thấp nhất của FGCRS, tỷ lệ nguy cơ khuyết tật được điều chỉnh đa biến là 1,34 (95% CI, 1,11-1,62) đối với phần ba cao nhất. Ngoài ra, FGCRS cao nhất có liên quan đến sự thay đổi điểm số hoạt động sinh hoạt hàng ngày theo thời gian (β = 0,057; 95% CI, 0,021-0,093). Mối liên quan giữa FGCRS và sự thay đổi trong các hoạt động sinh hoạt hàng ngày lần lượt là 13,8% do tích tụ CVD và 25,1% do suy giảm nhận thức. Kết luận Gánh nặng nguy cơ tim mạch cao hơn làm tăng nguy cơ khuyết tật và đẩy nhanh quá trình tiến triển của khuyết tật theo thời gian. Sự tích tụ CVD và suy giảm nhận thức có thể ảnh hưởng một phần đến mối liên quan này."} +{"idx": 1870141, "en": "OBJECTIVE A previous survey investigated postmenopausal vaginal atrophy in a sample of women across Latin America. To help implement a tailored approach to improve postmenopausal care and outcomes in Brazil, we consider results from the survey for this country. METHODS A total of 2509 postmenopausal women resident in Argentina, Brazil, Chile, Colombia, or Mexico completed an online questionnaire. The Brazilian cohort comprised 504 women. RESULTS Over half of the Brazilian cohort (56%) reported experiencing symptoms of vaginal atrophy; most described them as moderate or severe (76%), and almost half (48%) experienced symptoms for at least 1 year. Three-quarters of the Brazilian cohort (75%) were unaware of the chronic nature of the condition. Upon experiencing symptoms of vaginal atrophy, 92% had visited a health-care provider to discuss treatment options. Overall, 56% were aware of some form of local hormone therapy and 40% of those affected by vaginal atrophy had used such treatment. CONCLUSION Postmenopausal women in Brazil are likely to benefit from increased awareness of the symptoms of vaginal atrophy. Health-care providers can potentially improve outcomes by helping women to understand the chronic nature of the condition and available treatment options. Women may be open to education pre menopause, before symptoms occur.", "gemini": "MỤC TIÊU Một cuộc khảo sát trước đây đã điều tra tình trạng teo âm đạo sau mãn kinh ở một nhóm phụ nữ trên khắp Châu Mỹ Latinh. Để giúp thực hiện một cách tiếp cận phù hợp nhằm cải thiện việc chăm sóc sau mãn kinh và kết quả ở Brazil, chúng tôi xem xét kết quả từ cuộc khảo sát dành cho quốc gia này. PHƯƠNG PHÁP Tổng cộng 2509 phụ nữ sau mãn kinh cư trú tại Argentina, Brazil, Chile, Colombia hoặc Mexico đã hoàn thành bảng câu hỏi trực tuyến. Nhóm phụ nữ Brazil bao gồm 504 người. KẾT QUẢ Hơn một nửa nhóm phụ nữ Brazil (56%) cho biết họ gặp các triệu chứng teo âm đạo; hầu hết mô tả chúng ở mức độ trung bình hoặc nghiêm trọng (76%) và gần một nửa (48%) gặp các triệu chứng trong ít nhất 1 năm. Ba phần tư nhóm phụ nữ Brazil (75%) không biết về bản chất mãn tính của tình trạng này. Khi gặp các triệu chứng teo âm đạo, 92% đã đến gặp nhà cung cấp dịch vụ chăm sóc sức khỏe để thảo luận về các lựa chọn điều trị. Nhìn chung, 56% biết về một số hình thức liệu pháp hormone tại chỗ và 40% những người bị teo âm đạo đã sử dụng phương pháp điều trị như vậy. KẾT LUẬN Những phụ nữ sau mãn kinh ở Brazil có khả năng sẽ được hưởng lợi từ việc nâng cao nhận thức về các triệu chứng teo âm đạo. Các nhà cung cấp dịch vụ chăm sóc sức khỏe có khả năng cải thiện kết quả bằng cách giúp phụ nữ hiểu được bản chất mãn tính của tình trạng này và các lựa chọn điều trị có sẵn. Phụ nữ có thể cởi mở với việc giáo dục trước thời kỳ mãn kinh, trước khi các triệu chứng xảy ra."} +{"idx": 1870142, "en": "The ways in which residential aged care staff conceptualize the identities of residents with dementia has significance for how they support them to make decisions and make decisions on their behalf. This article aims to further understand how staff in residential aged care facilities comprehend who residents \"are.\" METHODS This qualitative study draws on individual and group interview data with aged care staff from two Australian states concerning decision making for individuals with dementia. It identifies themes relevant to how staff refer to identity in relation to such residents. RESULTS Staff possess disparate ideas about what things comprise residents' identities and, accordingly, which sources of knowledge are most relevant to learning about residents. DISCUSSION This article argues for the application of a multidimensional and temporally inclusive understanding of identity by those who provide care to people with dementia.", "gemini": "Cách mà nhân viên chăm sóc người cao tuổi tại nhà hiểu về danh tính của những người mắc chứng mất trí có ý nghĩa đối với cách họ hỗ trợ họ đưa ra quyết định và đưa ra quyết định thay mặt họ. Bài viết này nhằm mục đích hiểu sâu hơn về cách các nhân viên trong các cơ sở chăm sóc người cao tuổi tại nhà hiểu được \"con người\" của những người cư trú. PHƯƠNG PHÁP Nghiên cứu định tính này dựa trên dữ liệu phỏng vấn cá nhân và nhóm với nhân viên chăm sóc người cao tuổi từ hai tiểu bang của Úc liên quan đến việc ra quyết định cho những người mắc chứng mất trí. Nghiên cứu xác định các chủ đề có liên quan đến cách nhân viên đề cập đến danh tính liên quan đến những cư dân như vậy. KẾT QUẢ Các nhân viên có những ý tưởng khác nhau về những điều tạo nên danh tính của cư dân và do đó, nguồn kiến thức nào có liên quan nhất đến việc tìm hiểu về cư dân. THẢO LUẬN Bài viết này lập luận rằng những người chăm sóc những người mắc chứng mất trí nên áp dụng một sự hiểu biết đa chiều và bao gồm cả thời gian về danh tính."} +{"idx": 1870143, "en": "The reply of Hermida et al. (2020) to our critical comments on the MAPEC and HYGIA Lemmer and Middeke (2020) studies in this Journal is insufficient and incomplete. Hermida does not address our first and main point on the baseline blood pressure values of 131/77 mmHg over 48 hours comprising 57.4% of the treated hypertensives (according to Table 1 in HYGIA) and consequently 42.6% of the untretated hypertensives! We criticized that in the HYGIA study; both normal and treated patients were included in one group not separated by statistics and without information on the baseline blood pressure values in each subgroup. This basic failure is our key issue of criticism and the basis of unreliability concerning the whole publication. This issue was not picked up in the recent comment of Hermida et al. (2020). They just concentrated on minor points in order to reject our severe criticism.", "gemini": "Câu trả lời của Hermida et al. (2020) đối với những bình luận chỉ trích của chúng tôi về các nghiên cứu MAPEC và HYGIA Lemmer và Middeke (2020) trên Tạp chí này là không đầy đủ và chưa hoàn chỉnh. Hermida không đề cập đến quan điểm đầu tiên và chính của chúng tôi về các giá trị huyết áp cơ bản là 131/77 mmHg trong hơn 48 giờ, bao gồm 57,4% những người tăng huyết áp được điều trị (theo Bảng 1 trong HYGIA) và do đó là 42,6% những người tăng huyết áp không được điều trị! Chúng tôi chỉ trích rằng trong nghiên cứu HYGIA; cả bệnh nhân bình thường và bệnh nhân được điều trị đều được đưa vào một nhóm không được phân tách bằng số liệu thống kê và không có thông tin về các giá trị huyết áp cơ bản trong mỗi nhóm phụ. Sai sót cơ bản này là vấn đề chỉ trích chính của chúng tôi và là cơ sở cho sự không đáng tin cậy đối với toàn bộ ấn phẩm. Vấn đề này đã không được đề cập trong bình luận gần đây của Hermida et al. (2020). Họ chỉ tập trung vào những điểm nhỏ để bác bỏ lời chỉ trích nghiêm khắc của chúng tôi."} +{"idx": 1870144, "en": "INTRODUCTION Early-stage follicular lymphoma (FL) is characterized by good prognosis and can be cured with involved-field radiotherapy (IF-RT) in most cases. PET scan is a milestone of diagnostic work-up, with the aim of identifying a truly localized disease; however, staging in most of the studies was without PET. AREAS COVERED We have searched in MEDLINE (inclusive dates 1994-2020) data about localized FL management. While high-quality evidence is lacking, current guidelines recommend IFRT or involved-site RT as first-line treatment in limited stages FL. Since a significant proportion of disease relapse occurred in non-irradiated areas, it has been hypothesized that occult disease could be present at diagnosis and could persist after RT, contributing to relapse. Available treatment options include watch-and-wait, chemotherapy, RT plus chemo- or chemo-immunotherapy, and RT combined with rituximab (R). EXPERT OPINION RT combined with chemotherapy could increase PFS, but a clear OS benefit is lacking and toxic effects could be unacceptable. A promising strategy is represented by R combined with IF-RT, with low relapse rate outside the radiation fields and without the toxicity reported with chemotherapy. The study of prognostic factors in PET-staged patients, the reduction of RT fields and doses, and a response-adapted strategy represent new perspectives to investigate.", "gemini": "GIỚI THIỆU U lympho nang trứng (FL) giai đoạn đầu có đặc điểm là tiên lượng tốt và trong hầu hết các trường hợp có thể chữa khỏi bằng xạ trị có trường liên quan (IF-RT). Chụp PET là một cột mốc của quá trình chẩn đoán, nhằm mục đích xác định bệnh thực sự khu trú; tuy nhiên, việc phân giai đoạn trong hầu hết các nghiên cứu đều không có PET. PHẠM VI ĐƯỢC BAO PHỦ Chúng tôi đã tìm kiếm dữ liệu về quản lý FL khu trú trong MEDLINE (bao gồm các ngày từ 1994-2020). Mặc dù thiếu bằng chứng chất lượng cao, các hướng dẫn hiện hành khuyến cáo IFRT hoặc RT tại vị trí liên quan là phương pháp điều trị一线 ở các giai đoạn FL hạn chế. Vì một tỷ lệ đáng kể bệnh tái phát xảy ra ở những vùng không được chiếu xạ, nên người ta đưa ra giả thuyết rằng bệnh ẩn có thể có mặt khi chẩn đoán và có thể tồn tại sau RT, góp phần vào việc tái phát. Các lựa chọn điều trị hiện có bao gồm theo dõi và chờ đợi, hóa trị, RT cộng với hóa trị hoặc hóa trị miễn dịch và RT kết hợp với rituximab (R). Ý KIẾN CHUYÊN GIA RT kết hợp với hóa trị có thể làm tăng PFS, nhưng lợi ích OS rõ ràng là không có và các tác dụng độc hại có thể không được chấp nhận. Một chiến lược đầy hứa hẹn được thể hiện bằng R kết hợp với IF-RT, với tỷ lệ tái phát thấp bên ngoài vùng bức xạ và không có độc tính được báo cáo với hóa trị. Nghiên cứu về các yếu tố tiên lượng ở những bệnh nhân được phân giai đoạn bằng PET, việc giảm các trường và liều RT và một chiến lược thích ứng với đáp ứng là những góc nhìn mới cần được nghiên cứu."} +{"idx": 1870145, "en": "STUDY DESIGN Bibliometric analysis. OBJECTIVE Cervical spondylotic myelopathy (CSM) has become the most common cause of spinal cord dysfunction. Many topics of CSM still remain controversial. This study aimed to illustrate the overall knowledge structure and development trends of CSM. METHODS Research data sets were acquired from the Web of Science database and the time span was defined as \"2000 to 2019.\" VOS viewer and Citespace software was used to analyze the data and generate visualization knowledge maps. Annual trends of publications, distribution, H-index status, co-authorship status, and research hotspots were analyzed. RESULTS A total of 2367 publications met the requirement. The largest number of articles was from the United States, followed by Japan, China, Canada, and India. The highest H-index was found for articles from the United States. The highest number of articles was published in Spine. The cooperation between the countries, institutes, and authors were relatively weak. Cervical sagittal alignment, predictive factor, diffusion tensor imaging, and the natural history of CSM may become a frontier in this research field. CONCLUSION The number of publications showed an upward trend with a stable rise. Most of the publications are limited to a few countries and institutions with relatively weak interaction. The United States, Canada, Japan, China, and India have made significant contributions to the field of CSM. The United States is the country with the highest productivity, not only in quality but also in quantity. Cervical sagittal alignment, predictive factor, diffusion tensor imaging, and the natural history of CSM are the research hotspots in the recent years.", "gemini": "THIẾT KẾ NGHIÊN CỨU Phân tích thư mục. MỤC TIÊU Bệnh lý tủy cổ tử cung (CSM) đã trở thành nguyên nhân phổ biến nhất gây ra rối loạn chức năng tủy sống. Nhiều chủ đề của CSM vẫn còn gây tranh cãi. Nghiên cứu này nhằm mục đích minh họa cấu trúc tri thức tổng thể và xu hướng phát triển của CSM. PHƯƠNG PHÁP Các tập dữ liệu nghiên cứu được lấy từ cơ sở dữ liệu Web of Science và khoảng thời gian được xác định là \"2000 đến 2019.\" Phần mềm VOS viewer và Citespace được sử dụng để phân tích dữ liệu và tạo bản đồ kiến thức trực quan. Các xu hướng hàng năm về ấn phẩm, phân phối, trạng thái chỉ số H, trạng thái đồng tác giả và các điểm nóng nghiên cứu đã được phân tích. KẾT QUẢ Tổng cộng có 2367 ấn phẩm đáp ứng yêu cầu. Số lượng bài báo lớn nhất đến từ Hoa Kỳ, tiếp theo là Nhật Bản, Trung Quốc, Canada và Ấn Độ. Chỉ số H cao nhất được tìm thấy đối với các bài báo từ Hoa Kỳ. Số lượng bài báo được xuất bản nhiều nhất là ở Spine. Sự hợp tác giữa các quốc gia, viện nghiên cứu và tác giả tương đối yếu. Sự liên kết cung cổ tử cung, yếu tố dự đoán, hình ảnh tensor khuếch tán và tiền sử tự nhiên của CSM có thể trở thành một biên giới trong lĩnh vực nghiên cứu này. KẾT LUẬN Số lượng ấn phẩm có xu hướng tăng với tốc độ ổn định. Hầu hết các ấn phẩm chỉ giới hạn ở một số quốc gia và tổ chức với tương tác tương đối yếu. Hoa Kỳ, Canada, Nhật Bản, Trung Quốc và Ấn Độ đã có những đóng góp đáng kể cho lĩnh vực CSM. Hoa Kỳ là quốc gia có năng suất cao nhất, không chỉ về chất lượng mà còn về số lượng. Sự liên kết cung cổ tử cung, yếu tố dự đoán, hình ảnh tensor khuếch tán và tiền sử tự nhiên của CSM là những điểm nóng nghiên cứu trong những năm gần đây."} +{"idx": 1870146, "en": "Following the diagnosis of a falciparum malaria case imported from Djibouti and not detected by a pfHRP2-based rapid diagnostic test (RDT), we investigated the prevalence of the pfhrp2/pfhrp3-deleted parasites in Djibouti using 378 blood samples collected between January and May 2019, from Djiboutian patients with suspected malaria. Malaria diagnosis by quantitative PCR confirmed the presence of Plasmodium falciparum for 20.9% (79/378) samples while RDTs did not detect HRP2 antigen in 83.5% (66/79) of these samples. Quantitative PCRs targeting the pfhrp2/pfhrp3 genes confirmed the absence of both genes for 86.5% of P. falciparum strains. The very large number (86.5%) of falciparum parasites lacking the pfhrp2/pfhrp3 genes observed in this study, now justifies the use of non-HRP2 alternative RDTs in Djibouti. In this area and in most countries where HRP2-based RDTs constitute the main arsenal for falciparum malaria diagnosis, it is important to implement a systematic surveillance and to inform biologists and clinicians about the risk of malaria misdiagnosis. Further investigations are needed to better understand the mechanism of selection and diffusion of the pfhrp2/pfhrp3-deleted parasites.", "gemini": "Sau chẩn đoán một trường hợp sốt rét falciparum nhập khẩu từ Djibouti và không được phát hiện bằng xét nghiệm chẩn đoán nhanh dựa trên pfHRP2 (RDT), chúng tôi đã điều tra tỷ lệ lưu hành của ký sinh trùng bị xóa pfhrp2/pfhrp3 ở Djibouti bằng cách sử dụng 378 mẫu máu thu thập từ tháng 1 đến tháng 5 năm 2019, từ những bệnh nhân Djibouti nghi ngờ mắc bệnh sốt rét. Chẩn đoán sốt rét bằng PCR định lượng đã xác nhận sự hiện diện của Plasmodium falciparum đối với 20,9% (79/378) mẫu trong khi RDT không phát hiện kháng nguyên HRP2 ở 83,5% (66/79) trong số các mẫu này. Các PCR định lượng nhắm vào các gen pfhrp2/pfhrp3 đã xác nhận sự vắng mặt của cả hai gen đối với 86,5% các chủng P. falciparum. Số lượng rất lớn (86,5%) ký sinh trùng falciparum thiếu các gen pfhrp2/pfhrp3 được quan sát thấy trong nghiên cứu này, hiện biện minh cho việc sử dụng RDT thay thế không phải HRP2 ở Djibouti. Ở khu vực này và ở hầu hết các quốc gia mà RDT dựa trên HRP2 là vũ khí chính để chẩn đoán sốt rét falciparum, điều quan trọng là phải thực hiện giám sát có hệ thống và thông báo cho các nhà sinh học và bác sĩ lâm sàng về nguy cơ chẩn đoán nhầm sốt rét. Cần có thêm các cuộc điều tra để hiểu rõ hơn về cơ chế lựa chọn và khuếch tán của các ký sinh trùng bị xóa pfhrp2/pfhrp3."} +{"idx": 1870147, "en": "NCT02665052. Registered 27 January 2016. https://clinicaltrials.gov/ct2/show/NCT02665052.", "gemini": "NCT02665052. Đã đăng ký ngày 27 tháng 1 năm 2016. https://clinicaltrials.gov/ct2/show/NCT02665052."} +{"idx": 1870148, "en": "This paper presents a novel Bayesian variable selection approach that accounts for the sign of the regression coefficients based on multivariate one-sided tests. We propose a truncated g prior to specify a prior distribution of coefficients with anticipated signs in a given model. Informative priors for the direction of the effects can be incorporated into prior model probabilities. The best subset of variables is selected by comparing the posterior probabilities of the possible models. The new Bayesian one-sided variable selection procedure has higher chance to include relevant variables and therefore select the best model, if the anticipated direction is accurate. For a large number of candidate variables, we present an adaptation of a Bayesian model search method for the one-sided variable selection problem to ensure fast computation. In addition, a fully Bayesian approach is used to adjust the prior inclusion probability of each one-sided model to correct for multiplicity. The performance of the proposed method is investigated using several simulation studies and two real data examples.", "gemini": "Bài báo này trình bày một phương pháp chọn biến Bayesian mới có tính đến dấu hiệu của các hệ số hồi quy dựa trên các bài kiểm tra một bên đa biến. Chúng tôi đề xuất một g bị cắt cụt trước để chỉ định phân phối trước của các hệ số có các dấu hiệu dự đoán trong một mô hình nhất định. Các tiên nghiệm có thông tin về hướng của các hiệu ứng có thể được kết hợp vào các xác suất mô hình trước. Tập hợp con biến tốt nhất được chọn bằng cách so sánh các xác suất sau của các mô hình có thể. Quy trình chọn biến một bên Bayesian mới có khả năng bao gồm các biến có liên quan cao hơn và do đó chọn mô hình tốt nhất, nếu hướng dự đoán là chính xác. Đối với một số lượng lớn các biến ứng viên, chúng tôi trình bày một sự thích ứng của phương pháp tìm kiếm mô hình Bayesian cho vấn đề chọn biến một bên để đảm bảo tính toán nhanh. Ngoài ra, một phương pháp Bayesian hoàn toàn được sử dụng để điều chỉnh xác suất bao gồm trước của từng mô hình một bên để hiệu chỉnh cho tính đa dạng. Hiệu suất của phương pháp được đề xuất được nghiên cứu bằng cách sử dụng một số nghiên cứu mô phỏng và hai ví dụ dữ liệu thực."} +{"idx": 1870149, "en": "Two new selariscinins named selariscinin F (1) and selariscinin G (2), along with one known selariscinin D (3) were isolated from Selaginella tamariscina. The structures of 1-3 were elucidated on the basis of chemical and spectral analysis, including 1D, 2D NMR analyses and HRESIMS.", "gemini": "Hai selariscinin mới được đặt tên là selariscinin F (1) và selariscinin G (2), cùng với một selariscinin D (3) đã biết được phân lập từ Selaginella tamariscina. Các cấu trúc của 1-3 được làm sáng tỏ trên cơ sở phân tích hóa học và quang phổ, bao gồm phân tích NMR 1D, 2D và HRESIMS."} +{"idx": 1870150, "en": "INTRODUCTION Anthracycline-based chemotherapy regimens are associated with decreased cardiac function with cumulative dosing, yet there is limited information regarding the acute cardiotoxic potential of these medications and appropriate medical management strategies. Herein, we report a case of cardiomyopathy following a single dose of doxorubicin and describe our pharmacologic management approach. CASE REPORT A 37 year old Jamaican male presented for work-up and treatment of HTLV-1 associated T-cell leukemia/lymphoma. Upon diagnosis, the patient received one cycle of CHOEP, which was complicated by tumor lysis syndrome. Subsequently, the treatment was changed to DA-EPOCH, however, immediately after the initiation of DA-EPOCH on day 1, the patient was found to have t-wave inversions on EKG and an ejection fraction (EF) of 20% with new mitral regurgitation. EPOCH infusion was discontinued within 3 hours of initiation.Management and outcome: The chemotherapy regimen was modified to DA-EPOC with the removal of doxorubicin. The patient was started on metoprolol succinate 12.5 mg once daily for 2 days and subsequently switched to carvedilol 3.125 mg twice daily and lisinopril 5 mg once daily; the patient's ejection fraction improved to baseline after 2.5 months of therapy. DISCUSSION Though anthracyclines are associated with cardiotoxicity at high cumulative doses, this case highlights the cardiotoxic potential of these medications in the acute setting. Management of anthracycline cardiotoxicity is similar to heart failure management, with data suggesting benefit of using carvedilol and lisinopril. It is unclear if our patient would have benefited from prophylactic angiotensin converting enzymes inhibitors (ACEi) and/or beta-blocker therapy, as he had no known cardiac disease. Acute anthracycline-induced cardiac toxicity is an adverse drug reaction with which providers should be familiar and know how to appropriately manage.", "gemini": "GIỚI THIỆU Các phác đồ hóa trị dựa trên anthracycline có liên quan đến chức năng tim giảm khi dùng liều tích lũy, tuy nhiên thông tin về khả năng gây độc tim cấp tính của các loại thuốc này và các chiến lược quản lý y tế phù hợp còn hạn chế. Ở đây, chúng tôi báo cáo một trường hợp bệnh cơ tim sau một liều duy nhất doxorubicin và mô tả cách tiếp cận quản lý dược lý của chúng tôi. BÁO CÁO TRƯỜNG HỢP Một nam giới Jamaica 37 tuổi đến khám và điều trị bệnh bạch cầu/u lympho tế bào T liên quan đến HTLV-1. Khi chẩn đoán, bệnh nhân đã nhận được một chu kỳ CHOEP, phức tạp bởi hội chứng ly giải khối u. Sau đó, điều trị được chuyển sang DA-EPOCH, tuy nhiên, ngay sau khi bắt đầu điều trị DA-EPOCH vào ngày 1, bệnh nhân được phát hiện có sóng t đảo ngược trên EKG và phân suất tống máu (EF) là 20% với tình trạng trào ngược van hai lá mới. Truyền EPOCH đã được ngừng trong vòng 3 giờ sau khi bắt đầu. Quản lý và kết quả: Phác đồ hóa trị đã được sửa đổi thành DA-EPOC bằng cách loại bỏ doxorubicin. Bệnh nhân đã bắt đầu dùng metoprolol succinate 12,5 mg một lần mỗi ngày trong 2 ngày và sau đó chuyển sang carvedilol 3,125 mg hai lần mỗi ngày và lisinopril 5 mg một lần mỗi ngày; phân suất tống máu của bệnh nhân đã cải thiện trở lại mức ban đầu sau 2,5 tháng điều trị. BÀN LUẬN Mặc dù anthracycline có liên quan đến độc tính trên tim ở liều tích lũy cao, nhưng trường hợp này làm nổi bật khả năng gây độc tim của các loại thuốc này trong tình trạng cấp tính. Quản lý độc tính trên tim của anthracycline tương tự như quản lý suy tim, với dữ liệu cho thấy lợi ích của việc sử dụng carvedilol và lisinopril. Không rõ liệu bệnh nhân của chúng tôi có được hưởng lợi từ liệu pháp ức chế men chuyển angiotensin (ACEi) dự phòng và/hoặc liệu pháp chẹn beta hay không, vì ông không mắc bệnh tim đã biết. Độc tính tim cấp tính do anthracycline gây ra là một phản ứng có hại của thuốc mà các nhà cung cấp nên biết và biết cách quản lý thích hợp."} +{"idx": 1870151, "en": "BACKGROUND Glomerular IgG deposition in patients with IgA nephropathy (IgAN) has been shown to be associated with poor renal survival; however, most published studies to date are too small-scale and inconsistent to provide guidance for clinical practice. METHODS Based on renal biopsy findings, 742 patients were divided into the following groups: (i) IgA deposition alone (IgA) vs IgA + IgG deposition (IgA + IgG) and (ii) IgG co-deposition confined to the mesangium vs mesangium + capillary loops (CLs). The clinicopathological variables at biopsy and renal outcome were assessed. RESULTS Of the 742 patients, 182 had IgG co-deposition and 51 had IgG deposits in the mesangium + CLs. Patients with IgG co-deposition were associated with severe clinical and pathological lesions, especially those with a location of IgG deposits in the mesangium +CLs. Kaplan-Meier analysis revealed that a lower renal cumulative survival rate was present in both patients with IgG co-deposition and those with a location of IgG deposits in the mesangium + CLs (all p < 0.05). Moreover, patients with a higher intensity of glomerular IgG deposits or C3 deposits or C1q deposits were also associated with a lower survival rate. A multivariate Cox regression model identified the location of IgG deposits in the mesangium + CLs as an independent risk factor for poor prognosis (HR, 2.11; 95% CI: 1.06-4.18; p = 0.005). CONCLUSIONS Glomerular IgG co-deposition and the location of glomerular IgG deposits in the mesangium + CLs were both associated with adverse renal outcomes, but only the location of glomerular IgG deposits in the CLs was an independent risk factor for poor prognosis in IgAN.", "gemini": "BỐI CẢNH Sự lắng đọng IgG ở cầu thận ở những bệnh nhân mắc bệnh thận IgA (IgAN) đã được chứng minh là có liên quan đến sự sống còn kém của thận; tuy nhiên, hầu hết các nghiên cứu đã công bố cho đến nay đều có quy mô quá nhỏ và không nhất quán để đưa ra hướng dẫn cho thực hành lâm sàng. PHƯƠNG PHÁP Dựa trên kết quả sinh thiết thận, 742 bệnh nhân được chia thành các nhóm sau: (i) chỉ lắng đọng IgA (IgA) so với lắng đọng IgA + IgG (IgA + IgG) và (ii) đồng lắng đọng IgG giới hạn ở trung mô so với trung mô + quai mao mạch (CL). Các biến lâm sàng và bệnh lý tại thời điểm sinh thiết và kết quả về thận đã được đánh giá. KẾT QUẢ Trong số 742 bệnh nhân, 182 bệnh nhân có đồng lắng đọng IgG và 51 bệnh nhân có lắng đọng IgG ở trung mô + CL. Bệnh nhân có đồng lắng đọng IgG có liên quan đến các tổn thương lâm sàng và bệnh lý nghiêm trọng, đặc biệt là những bệnh nhân có vị trí lắng đọng IgG ở trung mô + CL. Phân tích Kaplan-Meier cho thấy tỷ lệ sống sót tích lũy của thận thấp hơn ở cả những bệnh nhân có đồng lắng đọng IgG và những bệnh nhân có vị trí lắng đọng IgG ở trung mô + CL (tất cả p < 0,05). Hơn nữa, những bệnh nhân có cường độ lắng đọng IgG ở cầu thận hoặc lắng đọng C3 hoặc lắng đọng C1q cao hơn cũng có tỷ lệ sống sót thấp hơn. Mô hình hồi quy Cox đa biến xác định vị trí lắng đọng IgG ở trung mô + CL là một yếu tố nguy cơ độc lập đối với tiên lượng kém (HR, 2,11; 95% CI: 1,06-4,18; p = 0,005). KẾT LUẬN Sự đồng lắng đọng IgG ở cầu thận và vị trí lắng đọng IgG ở cầu thận ở trung mô + CL đều liên quan đến kết quả về thận bất lợi, nhưng chỉ có vị trí lắng đọng IgG ở cầu thận ở CL là yếu tố nguy cơ độc lập đối với tiên lượng kém ở IgAN."} +{"idx": 1870152, "en": "A qualitative egocentric social network approach was taken to explore motivations for pre-exposure prophylaxis (PrEP)-related communication between women who inject drugs and network members. Eligible participants were HIV-negative, 18 years or older, and participating in a PrEP demonstration project in Philadelphia, PA, USA. The study employed content analysis of in-depth interviews to identify themes related to contextual and relational factors impacting PrEP communication within networks. Participants (n = 20) named on average three network members, resulting in a total of 57 unique relationships. PrEP conversations occurred within 30 of the 57 relationships, and motivations were to benefit others, to benefit themselves, and due to a sense of obligation. Some conversations also occurred when a peer unexpectedly found their pills. Taking a qualitative approach to network analysis provided a nuanced understanding of how interpersonal characteristics motivated PrEP conversations. Network interventions that facilitate information diffusion and social support may increase PrEP uptake and adherence among women who inject drugs.", "gemini": "Một cách tiếp cận mạng xã hội bản ngã định tính đã được thực hiện để khám phá động lực giao tiếp liên quan đến dự phòng trước phơi nhiễm (PrEP) giữa những phụ nữ tiêm chích ma túy và các thành viên trong mạng lưới. Những người tham gia đủ điều kiện là những người âm tính với HIV, từ 18 tuổi trở lên và đang tham gia một dự án trình diễn PrEP tại Philadelphia, PA, Hoa Kỳ. Nghiên cứu sử dụng phân tích nội dung các cuộc phỏng vấn chuyên sâu để xác định các chủ đề liên quan đến các yếu tố theo bối cảnh và quan hệ ảnh hưởng đến giao tiếp về PrEP trong các mạng lưới. Những người tham gia (n = 20) nêu tên trung bình ba thành viên trong mạng lưới, dẫn đến tổng cộng 57 mối quan hệ duy nhất. Các cuộc trò chuyện về PrEP diễn ra trong 30 trong số 57 mối quan hệ và động lực là để có lợi cho người khác, để có lợi cho bản thân và do cảm giác có nghĩa vụ. Một số cuộc trò chuyện cũng xảy ra khi một người bạn đồng trang lứa bất ngờ tìm thấy thuốc của họ. Việc áp dụng phương pháp định tính vào phân tích mạng lưới đã cung cấp sự hiểu biết sắc thái về cách các đặc điểm giữa các cá nhân thúc đẩy các cuộc trò chuyện về PrEP. Các can thiệp mạng lưới tạo điều kiện cho việc phổ biến thông tin và hỗ trợ xã hội có thể làm tăng tỷ lệ sử dụng PrEP và tuân thủ điều trị ở những phụ nữ tiêm chích ma túy."} +{"idx": 1870153, "en": "Sleepiness is an important consideration for workplace safety, especially in relation to shift work. There is limited understanding of how practical applications of countermeasures are used to manage sleepiness in a professional setting. One under-researched group is city bus drivers. This qualitative study investigated the use of individual sleepiness countermeasures within a sample of city bus drivers. Nine semi-structured focus groups were convened to explore the broader experience and management of sleepiness (n = 62, largest proportion aged 45-54 y, mean bus driving experience 13.3 y). The conversations of the focus groups were audio-recorded (total: 682 min) and transcribed anonymously verbatim. Discussions specifically relating to personal countermeasure use were isolated from the original transcripts, creating nine new transcripts for a targeted analysis on the topic of individual countermeasure use. Thematic analysis identified two main themes: (1) strategies used to counteract sleepiness and (2) barriers to individual countermeasure use, each with several subthemes. A variety of countermeasures were used, including strategies with limited potential for counteracting sleepiness, such as opening a window, drinking water, talking, stretching, and consuming forms of sugar. Workplace restrictions, such as access to facilities, limitations of food/drink consumption, and tight schedules, were the strongest influences on countermeasure choice. It is important that bus drivers have access to, and are aware of the effectiveness of, countermeasures to manage sleepiness during shift work. It is vital that the actions of drivers and shift workers are better understood in the planning of organizational countermeasures.", "gemini": "Buồn ngủ là một vấn đề quan trọng cần xem xét đối với sự an toàn tại nơi làm việc, đặc biệt là khi làm việc theo ca. Hiểu biết về cách thức sử dụng các biện pháp đối phó thực tế để quản lý tình trạng buồn ngủ trong môi trường làm việc chuyên nghiệp còn hạn chế. Một nhóm chưa được nghiên cứu đầy đủ là tài xế xe buýt thành phố. Nghiên cứu định tính này đã điều tra việc sử dụng các biện pháp đối phó với tình trạng buồn ngủ ở từng cá nhân trong một nhóm tài xế xe buýt thành phố. Chín nhóm thảo luận chuyên sâu bán cấu trúc đã được triệu tập để khám phá kinh nghiệm và cách quản lý tình trạng buồn ngủ rộng hơn (n = 62, tỷ lệ lớn nhất ở độ tuổi 45-54, kinh nghiệm lái xe buýt trung bình là 13,3 năm). Các cuộc trò chuyện của các nhóm thảo luận chuyên sâu đã được ghi âm (tổng cộng: 682 phút) và phiên âm ẩn danh từng chữ. Các cuộc thảo luận liên quan cụ thể đến việc sử dụng biện pháp đối phó cá nhân đã được tách riêng khỏi các bản ghi gốc, tạo ra chín bản ghi mới để phân tích mục tiêu về chủ đề sử dụng biện pháp đối phó cá nhân. Phân tích theo chủ đề xác định hai chủ đề chính: (1) các chiến lược được sử dụng để chống lại tình trạng buồn ngủ và (2) những rào cản đối với việc sử dụng các biện pháp đối phó cá nhân, mỗi chủ đề có một số chủ đề phụ. Nhiều biện pháp đối phó đã được sử dụng, bao gồm các chiến lược có tiềm năng hạn chế trong việc chống lại tình trạng buồn ngủ, chẳng hạn như mở cửa sổ, uống nước, nói chuyện, căng cơ và tiêu thụ các dạng đường. Các hạn chế tại nơi làm việc, chẳng hạn như tiếp cận các tiện nghi, hạn chế tiêu thụ thực phẩm/đồ uống và lịch trình dày đặc, là những yếu tố ảnh hưởng mạnh nhất đến việc lựa chọn biện pháp đối phó. Điều quan trọng là tài xế xe buýt phải được tiếp cận và nhận thức được hiệu quả của các biện pháp đối phó để quản lý tình trạng buồn ngủ trong quá trình làm việc theo ca. Điều quan trọng là phải hiểu rõ hơn về hành động của người lái xe và người làm việc theo ca trong việc lập kế hoạch các biện pháp đối phó của tổ chức."} +{"idx": 1870154, "en": "BACKGROUND The novel SARS-CoV-2 coronavirus, the causative agent of the ongoing pandemic COVID-19 disease continues to infect people globally and has infected millions of humans worldwide. However, no effective vaccine against this virus exists. METHOD Using Immunoinformatics, epitopic sequences from multiple glycoproteins that play crucial role in pathogenesis were identified. Particularly, epitopes were mapped from conserved receptor-binding domain of spike protein which have been experimentally validated in SARS-CoV-1 as a promising target for vaccine development. RESULTS A multi-epitopic vaccine construct comprising of B-cell, CTL, HTL epitopes was developed along with fusion of adjuvant and linkers. The epitopes identified herein are reported for the first time and were predicted to be highly antigenic, stable, nonallergen, nontoxic and displayed conservation across several SARS-CoV-2 isolates from different countries. Additionally, the epitopes associated with maximum HLA alleles and population coverage analysis shows the proposed epitopes would be a relevant representative of large proportion of the world population. A reliable three-dimensional structure of the vaccine construct was developed. Consequently, docking and molecular-dynamics simulation ensured the stable interaction between vaccine and innate-immune receptor.", "gemini": "BỐI CẢNH: Loại vi-rút SARS-CoV-2 mới, tác nhân gây ra đại dịch COVID-19 đang diễn ra, tiếp tục lây nhiễm cho mọi người trên toàn cầu và đã lây nhiễm cho hàng triệu người trên toàn thế giới. Tuy nhiên, hiện vẫn chưa có vắc-xin hiệu quả chống lại loại vi-rút này. PHƯƠNG PHÁP: Sử dụng Miễn dịch học, các trình tự biểu hiện từ nhiều glycoprotein đóng vai trò quan trọng trong quá trình sinh bệnh đã được xác định. Đặc biệt, các epitop được lập bản đồ từ miền liên kết thụ thể được bảo tồn của protein gai, đã được xác nhận bằng thực nghiệm ở SARS-CoV-1 là mục tiêu đầy hứa hẹn để phát triển vắc-xin. KẾT QUẢ: Một cấu trúc vắc-xin đa biểu hiện bao gồm các biểu hiện B-cell, CTL, HTL đã được phát triển cùng với sự kết hợp của tá dược và chất kết nối. Các epitop được xác định trong tài liệu này được báo cáo lần đầu tiên và được dự đoán là có tính kháng nguyên cao, ổn định, không gây dị ứng, không độc hại và thể hiện sự bảo tồn trên một số phân lập SARS-CoV-2 từ các quốc gia khác nhau. Ngoài ra, các epitop liên quan đến các alen HLA tối đa và phân tích phạm vi dân số cho thấy các epitop được đề xuất sẽ là đại diện có liên quan của phần lớn dân số thế giới. Một cấu trúc ba chiều đáng tin cậy của cấu trúc vắc-xin đã được phát triển. Do đó, mô phỏng ghép nối và động lực phân tử đảm bảo sự tương tác ổn định giữa vắc-xin và thụ thể miễn dịch bẩm sinh."} +{"idx": 1870155, "en": "Background: It is beneficial for CI patients listen to music. However it is necessary to take steps to improve the musicality of CI patients.Objectives: The aims of the study were to evaluate the primary musicality of children with cochlear implants versus those with normal hearing.Material and methods: Children participating in this study were divided into two groups: the cochlear implant group (CI group) and the normal hearing group (NH group). The 'Musical Ears Evaluation Form for Professionals' was used to evaluate the subjects' primary musicality.Results: The scores for overall and the three subcategories of primary musicality in children with cochlear implants and in those with normal hearing also improved significantly over time (p < .05). The score for overall primary musicality was not significantly different between CI and NH groups in the same hearing age (p > .05). There were significant differences between the two groups in the same chronological age (p < .05).Conclusions and significance: The primary musicality in children with cochlear implants was not significantly different from normal hearing ones at the same hearing age. The primary musicality in children with cochlear implants was significantly lower than that of children with normal hearing at the same chronological age.", "gemini": "Bối cảnh: Bệnh nhân CI nghe nhạc có lợi. Tuy nhiên, cần phải thực hiện các bước để cải thiện khả năng âm nhạc của bệnh nhân CI. Mục tiêu: Mục đích của nghiên cứu là đánh giá khả năng âm nhạc chính của trẻ em có cấy ghép ốc tai điện tử so với trẻ em có thính lực bình thường. Vật liệu và phương pháp: Trẻ em tham gia nghiên cứu này được chia thành hai nhóm: nhóm cấy ghép ốc tai điện tử (nhóm CI) và nhóm thính lực bình thường (nhóm NH). 'Mẫu đánh giá thính giác âm nhạc cho các chuyên gia' được sử dụng để đánh giá khả năng âm nhạc chính của các đối tượng. Kết quả: Điểm tổng thể và ba tiểu thể loại khả năng âm nhạc chính ở trẻ em có cấy ghép ốc tai điện tử và ở trẻ em có thính lực bình thường cũng được cải thiện đáng kể theo thời gian (p <0,05). Điểm khả năng âm nhạc chính tổng thể không có sự khác biệt đáng kể giữa nhóm CI và nhóm NH ở cùng độ tuổi nghe (p> 0,05). Có sự khác biệt đáng kể giữa hai nhóm ở cùng độ tuổi theo năm (p <0,05). Kết luận và ý nghĩa: Khả năng âm nhạc chính ở trẻ em có cấy ghép ốc tai điện tử không có sự khác biệt đáng kể so với trẻ em có thính lực bình thường ở cùng độ tuổi nghe. Khả năng âm nhạc chính ở trẻ em có cấy ghép ốc tai điện tử thấp hơn đáng kể so với trẻ em có thính lực bình thường ở cùng độ tuổi theo năm."} +{"idx": 1870156, "en": "The pandemic of respiratory disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is life-threatening in peritoneal dialysis (PD) patients. In PD patients with systemic viral infections, peritoneal effluent may be theoretically contaminated. We searched for the presence of SARS-CoV-2 genetic material by real-time reverse transcriptase-polymerase chain reaction assays in serial PD effluents of three PD infected patients. Nasopharyngeal swabs obtained at admission showed high viral load in all three patients, whereas none of the PD effluent specimen tested positive, even after dialysate concentration. Those results support at most a very low SARS-CoV-2 dissemination risk by the peritoneal effluent of PD patients. Imposing special disposal procedures, such as the instillation of hypochlorite in the drainage bags to prevent viral spread to health-care workers, are probably not required.", "gemini": "Đại dịch bệnh đường hô hấp (COVID-19) do virus corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2) gây đe dọa tính mạng ở những bệnh nhân thẩm phân phúc mạc (PD). Ở những bệnh nhân PD bị nhiễm trùng do vi-rút toàn thân, dịch thẩm phúc mạc về mặt lý thuyết có thể bị nhiễm bẩn. Chúng tôi đã tìm kiếm sự hiện diện của vật liệu di truyền SARS-CoV-2 bằng phản ứng chuỗi polymerase phiên mã ngược thời gian thực trong các dịch thẩm phúc mạc nối tiếp của ba bệnh nhân PD bị nhiễm bệnh. Các mẫu tăm hầu họng lấy khi nhập viện cho thấy tải lượng vi-rút cao ở cả ba bệnh nhân, trong khi không có mẫu dịch thẩm phúc mạc nào có kết quả xét nghiệm dương tính, ngay cả sau khi cô đặc dịch thẩm phân. Những kết quả đó hỗ trợ rằng nguy cơ lây lan SARS-CoV-2 qua dịch thẩm phúc mạc của bệnh nhân PD rất thấp. Có lẽ không cần áp dụng các quy trình xử lý đặc biệt, chẳng hạn như nhỏ thuốc hypochlorite vào túi dẫn lưu để ngăn ngừa lây lan vi-rút cho nhân viên y tế."} +{"idx": 1870157, "en": "Individuals who have a strong locomotion orientation tend to be future-oriented and motivated to move from the present state toward a future state, making swift and steady progress toward their goals. The current study has assessed the conceptual possibility that such motivation leads locomotors to experience greater hopeful thinking, an active cognitive process that consists in planning the future and implementing these plans. The results of Study 1 lend preliminary support to this possibility. The practical implication was linked to the importance of hope in the subjective well-being experienced in everyday life. For this reason, Study 2 tested a model in which hopeful thinking mediated the subjective well-being experienced by locomotors. The results supported the hypothesis: locomotion orientation was associated with enhanced hope capabilities that, in turn, were associated with higher subjective well-being, with significant positive implications for individuals.", "gemini": "Những người có xu hướng định hướng vận động mạnh thường hướng đến tương lai và có động lực để chuyển từ trạng thái hiện tại sang trạng thái tương lai, tiến triển nhanh chóng và ổn định hướng tới mục tiêu của họ. Nghiên cứu hiện tại đã đánh giá khả năng khái niệm rằng động lực như vậy khiến những người vận động có suy nghĩ hy vọng hơn, một quá trình nhận thức tích cực bao gồm việc lập kế hoạch cho tương lai và thực hiện các kế hoạch này. Kết quả của Nghiên cứu 1 đưa ra sự hỗ trợ ban đầu cho khả năng này. Ý nghĩa thực tiễn có liên quan đến tầm quan trọng của hy vọng trong hạnh phúc chủ quan được trải nghiệm trong cuộc sống hàng ngày. Vì lý do này, Nghiên cứu 2 đã kiểm tra một mô hình trong đó suy nghĩ hy vọng đóng vai trò trung gian cho hạnh phúc chủ quan mà những người vận động trải nghiệm. Kết quả hỗ trợ cho giả thuyết: định hướng vận động có liên quan đến khả năng hy vọng được nâng cao, từ đó có liên quan đến hạnh phúc chủ quan cao hơn, với những ý nghĩa tích cực đáng kể đối với các cá nhân."} +{"idx": 1870158, "en": "Publications addressing aluminum (Al)-induced reproductive toxicity were reviewed. Key details were compiled in summary tables. Approximate systemic Al exposure, a measure of bioavailability, was calculated for each exposure, based on the Al percentage in the dosed Al species, Al bioavailability, and absorption time course reports for the exposure route. This was limited to laboratory animal studies because no controlled-exposure human studies were found. Intended Al exposure was compared to unintended dietary Al exposure. The considerable and variable Al content of laboratory animal diets creates uncertainty about reproductive function in the absence of Al. Aluminum-induced reproductive toxicity in female mice and rats was evident after exposure to ≥25-fold the amount of Al consumed in the diet. Generally, the additional daily Al systemic exposure of studies that reported statistically significant results was greater than 100-fold above the typical human daily Al dietary consumption equivalent. Male reproductive endpoints were significantly affected after exposure to lower levels of Al than females. Increased Al intake increased fetus, placenta, and testes Al concentrations, to a greater extent in the placenta than fetus, and, in some cases, more in the testes than placenta. An adverse outcome pathway (AOP) was constructed for males based on the results of the reviewed studies. The proposed AOP includes oxidative stress as the molecular initiating event and increased malondialdehyde, DNA and spermatozoal damage, and decreased blood testosterone and sperm count as subsequent key events. Recommendations for the design of future studies of reproductive outcomes following exposure to Al are provided.", "gemini": "Các ấn phẩm đề cập đến độc tính sinh sản do nhôm (Al) gây ra đã được xem xét. Các chi tiết chính được tổng hợp trong các bảng tóm tắt. Lượng phơi nhiễm Al toàn thân gần đúng, một thước đo về khả dụng sinh học, được tính cho mỗi lần phơi nhiễm, dựa trên tỷ lệ phần trăm Al trong các loài Al đã dùng, khả dụng sinh học của Al và các báo cáo về thời gian hấp thụ cho con đường phơi nhiễm. Điều này chỉ giới hạn ở các nghiên cứu trên động vật trong phòng thí nghiệm vì không tìm thấy nghiên cứu nào trên người tiếp xúc có kiểm soát. Lượng phơi nhiễm Al dự kiến được so sánh với lượng phơi nhiễm Al không chủ ý trong chế độ ăn uống. Hàm lượng Al đáng kể và thay đổi trong chế độ ăn của động vật trong phòng thí nghiệm tạo ra sự không chắc chắn về chức năng sinh sản khi không có Al. Độc tính sinh sản do nhôm gây ra ở chuột và chuột cái đã được chứng minh sau khi tiếp xúc với lượng Al gấp ≥25 lần lượng Al tiêu thụ trong chế độ ăn. Nhìn chung, lượng phơi nhiễm Al toàn thân hàng ngày bổ sung của các nghiên cứu báo cáo kết quả có ý nghĩa thống kê lớn hơn 100 lần so với lượng tiêu thụ Al hàng ngày thông thường của con người. Các điểm cuối sinh sản của nam giới bị ảnh hưởng đáng kể sau khi tiếp xúc với mức Al thấp hơn so với nữ giới. Lượng Al tăng lên làm tăng nồng độ Al ở thai nhi, nhau thai và tinh hoàn, ở nhau thai cao hơn ở thai nhi và trong một số trường hợp, ở tinh hoàn nhiều hơn ở nhau thai. Một con đường kết quả bất lợi (AOP) đã được xây dựng cho nam giới dựa trên kết quả của các nghiên cứu được xem xét. AOP được đề xuất bao gồm stress oxy hóa là sự kiện khởi đầu phân tử và tăng malondialdehyde, tổn thương DNA và tinh trùng, giảm testosterone trong máu và số lượng tinh trùng là các sự kiện chính tiếp theo. Các khuyến nghị về thiết kế các nghiên cứu trong tương lai về kết quả sinh sản sau khi tiếp xúc với Al được đưa ra."} +{"idx": 1870159, "en": "A large number of studies have examined predictors of female crime quantities yet considerably less attention has been directed toward exploring patterns in the nature or quality of female violence within and across communities. Although research consistently demonstrates that females engage in less criminal behavior than males, research on the variability across contexts is somewhat sparse. The authors conduct analyses to determine if Anderson's initial observations of female violence in neighborhoods inundated with the code of the streets persist a decade after his initial ethnographic account. Specifically, we examine incident-level data from the National Incident Based Reporting System with contextual-level data on the cities in which the incidents occurred. We use hierarchical linear and nonlinear modeling techniques to explore variations in predictors of offender gun use and extent of victim injury in violent female encounters. Supporting Anderson's initial accounts for street females and prior research we find the probability of gun usage and level of victim injury is not significantly influenced by differences in community context, and specifically not exacerbated by the types of conditions that make the code of the street locally salient.", "gemini": "Nhiều nghiên cứu đã xem xét các yếu tố dự báo về số lượng tội phạm của phụ nữ nhưng ít chú ý đến việc khám phá các mô hình về bản chất hoặc chất lượng bạo lực của phụ nữ trong và giữa các cộng đồng. Mặc dù các nghiên cứu liên tục chứng minh rằng phụ nữ có hành vi phạm tội ít hơn nam giới, nhưng các nghiên cứu về sự thay đổi theo bối cảnh lại khá ít. Các tác giả tiến hành phân tích để xác định xem những quan sát ban đầu của Anderson về bạo lực của phụ nữ ở những khu phố tràn ngập luật lệ đường phố có còn tồn tại hay không một thập kỷ sau báo cáo dân tộc học ban đầu của ông. Cụ thể, chúng tôi kiểm tra dữ liệu cấp độ sự cố từ Hệ thống báo cáo sự cố dựa trên sự cố quốc gia với dữ liệu cấp độ bối cảnh về các thành phố nơi xảy ra các sự cố. Chúng tôi sử dụng các kỹ thuật mô hình tuyến tính và phi tuyến phân cấp để khám phá các biến thể trong các yếu tố dự báo về việc sử dụng súng của người phạm tội và mức độ thương tích của nạn nhân trong các cuộc chạm trán bạo lực của phụ nữ. Hỗ trợ cho các báo cáo ban đầu của Anderson về phụ nữ đường phố và nghiên cứu trước đây, chúng tôi thấy rằng khả năng sử dụng súng và mức độ thương tích của nạn nhân không bị ảnh hưởng đáng kể bởi sự khác biệt về bối cảnh cộng đồng, và cụ thể là không bị trầm trọng hơn bởi các loại điều kiện khiến luật lệ của đường phố trở nên nổi bật tại địa phương."} +{"idx": 1870160, "en": "We report a case in which endovascular treatment (EVT) was performed for vertebral and basilar artery (VA and BA) tandem occlusion beyond 24 hours from onset of stroke. A 78-year-old man was admitted to our institution with dysarthria and disturbance of gait. MRI revealed occlusion of the BA with acute ischemic change in bilateral cerebellum and brain stem. At 36 hours after onset and 30 hours after administration, EVT was performed because of deteriorating neurological symptom. Successful revascularization was achieved with percutaneous transluminal angioplasty for VA and thrombectomy for BA occlusion. The neurological symptoms were improved in postoperative course.", "gemini": "Chúng tôi báo cáo một trường hợp trong đó điều trị nội mạch (EVT) được thực hiện cho tắc nghẽn động mạch đốt sống và động mạch đáy (VA và BA) song song sau 24 giờ kể từ khi khởi phát đột quỵ. Một người đàn ông 78 tuổi được đưa vào viện của chúng tôi với tình trạng nói khó và rối loạn dáng đi. MRI cho thấy tắc nghẽn BA với thay đổi thiếu máu cục bộ cấp tính ở cả hai tiểu não và thân não. 36 giờ sau khi khởi phát và 30 giờ sau khi dùng thuốc, EVT được thực hiện do các triệu chứng thần kinh xấu đi. Tái thông mạch thành công đã đạt được với nong mạch qua da cho VA và lấy huyết khối cho tắc nghẽn BA. Các triệu chứng thần kinh đã được cải thiện trong quá trình hậu phẫu."} +{"idx": 1870161, "en": "Although migraine is a major global public health problem, its impact on cognitive abilities remains controversial. Thus, the present study investigated the effects of repeated administration of inflammatory soup to the dura of rats, over three weeks, on spatial cognition, hippocampal synaptic plasticity, and the expression of N-methyl-D-aspartate receptor subunits. Additionally, low doses of amitriptyline (5 mg/kg) were applied to assess its therapeutic effects. The inflammatory soup group exhibited significant reductions in the cutaneous stimulation threshold, presence of mild cognitive impairment, and decreased long-term potentiation in right hippocampus. However, amitriptyline improved pain behaviors, enhanced cognitive function, and increased synaptic plasticity in the inflammatory soup rats. On the other hand, the administration of amitriptyline to normal rats negatively influenced synaptic plasticity and reduced the expression of N-methyl-D-aspartate receptor subunits. The present results indicate that inflammatory soup-induced dural nociception led to impairments in spatial cognition that could be attributed to reductions in hippocampal long-term potentiation and the decreased expression of N-methyl-D-aspartate receptor subunits.", "gemini": "Mặc dù đau nửa đầu là một vấn đề sức khỏe cộng đồng toàn cầu, nhưng tác động của nó đối với khả năng nhận thức vẫn còn gây tranh cãi. Do đó, nghiên cứu hiện tại đã điều tra tác động của việc dùng súp gây viêm nhiều lần vào màng cứng của chuột trong hơn ba tuần đối với nhận thức không gian, tính dẻo của khớp thần kinh ở hồi hải mã và biểu hiện của các tiểu đơn vị thụ thể N-methyl-D-aspartat. Ngoài ra, liều thấp amitriptyline (5 mg/kg) đã được sử dụng để đánh giá tác dụng điều trị của nó. Nhóm súp gây viêm cho thấy ngưỡng kích thích da giảm đáng kể, có suy giảm nhận thức nhẹ và giảm tiềm năng hóa lâu dài ở hồi hải mã phải. Tuy nhiên, amitriptyline cải thiện hành vi đau, tăng cường chức năng nhận thức và tăng tính dẻo của khớp thần kinh ở những con chuột dùng súp gây viêm. Mặt khác, việc dùng amitriptyline cho những con chuột bình thường ảnh hưởng tiêu cực đến tính dẻo của khớp thần kinh và làm giảm biểu hiện của các tiểu đơn vị thụ thể N-methyl-D-aspartat. Các kết quả hiện tại chỉ ra rằng đau màng cứng do súp gây viêm dẫn đến suy giảm nhận thức không gian có thể là do giảm tiềm năng hóa lâu dài của hồi hải mã và giảm biểu hiện của các tiểu đơn vị thụ thể N-methyl-D-aspartat."} +{"idx": 1870162, "en": "PURPOSE To evaluate the safety and feasibility of the in situ needle fenestration (ISNF) technique for reconstruction of the left subclavian artery (LSA) during thoracic endovascular aortic repair (TEVAR) of complicated aortic arch pathologies. MATERIALS AND METHODS A retrospective review was conducted from January 2014 to December 2019 of 50 patients (mean age 60.2±11.1; 45 men) who underwent ISNF to revascularize the LSA during TEVAR. Twenty-one of the patients also required revascularization of the left common carotid artery (LCCA; n=19) and innominate artery (IA; n=2) using physician-modified in vitro fenestration. Overall, 73 supra-aortic branches were targeted for revascularization. RESULTS ISNF was successful in 48 patients (96%); one LSA could not be stented and a tortuous LSA prevented the needle from fenestrating the graft. No perioperative major adverse event occurred. There were no type I and 4 type III endoleaks (8%), 3 of which occurred among the first 20 cases. Types II and IV endoleaks were found in 3 (6%) and 6 (12%) cases, respectively; all disappeared during a median follow-up of 15 months (range 3-66). One death (2%) occurred within 12 months due to cerebral hemorrhage. Two patients (4%) required open reinterventions at 6 and 62 months. CONCLUSION ISNF for revascularization of the LSA during TEVAR seems to be feasible with acceptable midterm outcomes. The learning curve and evolving patient selection criteria affected technical success, complications, and the need for reinterventions. Long-term durability requires further evaluation.", "gemini": "MỤC ĐÍCH Đánh giá tính an toàn và khả thi của kỹ thuật tạo lỗ thông kim tại chỗ (ISNF) để tái tạo động mạch dưới đòn trái (LSA) trong quá trình sửa chữa động mạch chủ bằng phương pháp nội mạch lồng stent ngực (TEVAR) của các bệnh lý cung động mạch chủ phức tạp. VẬT LIỆU VÀ PHƯƠNG PHÁP Đã tiến hành đánh giá hồi cứu từ tháng 1 năm 2014 đến tháng 12 năm 2019 đối với 50 bệnh nhân (tuổi trung bình 60,2 ± 11,1; 45 nam) đã trải qua ISNF để tái thông mạch LSA trong quá trình TEVAR. Hai mươi mốt trong số các bệnh nhân cũng cần tái thông mạch động mạch cảnh chung trái (LCCA; n = 19) và động mạch không tên (IA; n = 2) bằng cách sử dụng kỹ thuật tạo lỗ thông in vitro đã được bác sĩ chỉnh sửa. Nhìn chung, 73 nhánh trên động mạch chủ được nhắm mục tiêu để tái thông mạch. KẾT QUẢ ISNF đã thành công ở 48 bệnh nhân (96%); một LSA không thể đặt stent và một LSA ngoằn ngoèo đã ngăn không cho kim tạo lỗ thông trên ghép. Không có biến cố bất lợi lớn nào trong thời kỳ quanh phẫu thuật. Không có rò rỉ nội mạc loại I và 4 loại III (8%), trong đó 3 trường hợp xảy ra trong số 20 trường hợp đầu tiên. Các rò rỉ nội mạc loại II và IV được phát hiện ở 3 (6%) và 6 (12%) trường hợp; tất cả đều biến mất trong thời gian theo dõi trung bình là 15 tháng (phạm vi 3-66). Một trường hợp tử vong (2%) xảy ra trong vòng 12 tháng do xuất huyết não. Hai bệnh nhân (4%) cần phẫu thuật lại mở sau 6 và 62 tháng. KẾT LUẬN ISNF để tái thông mạch LSA trong quá trình TEVAR có vẻ khả thi với kết quả trung hạn có thể chấp nhận được. Đường cong học tập và các tiêu chí lựa chọn bệnh nhân đang phát triển ảnh hưởng đến thành công về mặt kỹ thuật, các biến chứng và nhu cầu phẫu thuật lại. Độ bền lâu dài cần được đánh giá thêm."} +{"idx": 1870163, "en": "OBJECTIVES Endovascular procedures are now the standard of care for endovascular aortic aneurysm repair but treatment of hostile proximal neck anatomy is still challenging. New endografts were brought to the market specifically designed to accommodate severe neck angulation. Authors describe a preliminary experience and early results using the Gore Excluder Conformable endograft (W. L. Gore & Associates, Flagstaff, Ariz) and its active control system in severe neck angulation with a standardized technical approach to achieve precise deployment in this hostile anatomy. METHODS From June 2019 to May 2020, five patients with abdominal aortic aneurysm and severe neck angulation (≥70°) were treated with the Gore Excluder Conformable endograft at two different centers. Deployment of this endograft in this kind of anatomy should be aggressive starting over the level of renal arteries due to risk of distal migration downward on the external curvature and difficulty in upward repositioning. Authors suggested a standard technique with a routine through-and-through axillary-femoral approach, using a floppy guidewire together with preventive cannulation of the lower renal artery if a short neck (<15 mm) is associated. RESULTS Endovascular aortic procedures were successfully completed in all patients. Final deployment of the stent graft using our standardized technical approach was extremely precise in all cases even if redeployment of the graft was necessary in all cases. No other secondary procedures were needed. At 30 days, no type IA endoleak was recorded and no aneurysm-related secondary procedures were performed. Median follow-up for this group of patients was 5.2 months (range 1-11). Early results revealed no type IA endoleak and no migration at Ct angiogram. No aneurysm-related secondary procedures were required. CONCLUSION Routine use of through-and-through axillary-femoral guidewire associated with selective pre-cannulation of the lower renal artery allows a precise deployment of the Gore Excluder Conformable endograft in difficult anatomies possibly affecting early outcomes.", "gemini": "MỤC TIÊU Các thủ thuật nội mạch hiện là tiêu chuẩn chăm sóc để sửa chữa phình động mạch chủ bằng phương pháp nội mạch nhưng điều trị giải phẫu cổ gần thù địch vẫn còn nhiều thách thức. Các ghép nội mạc mới đã được đưa ra thị trường được thiết kế đặc biệt để thích ứng với góc cổ nghiêm trọng. Các tác giả mô tả kinh nghiệm sơ bộ và kết quả ban đầu khi sử dụng ghép nội mạc Gore Excluder Conformable (W. L. Gore & Associates, Flagstaff, Ariz) và hệ thống kiểm soát chủ động của nó ở góc cổ nghiêm trọng với phương pháp tiếp cận kỹ thuật chuẩn hóa để đạt được triển khai chính xác trong giải phẫu thù địch này. PHƯƠNG PHÁP Từ tháng 6 năm 2019 đến tháng 5 năm 2020, năm bệnh nhân bị phình động mạch chủ bụng và góc cổ nghiêm trọng (≥70°) đã được điều trị bằng ghép nội mạc Gore Excluder Conformable tại hai trung tâm khác nhau. Việc triển khai ghép nội mạc này trong loại giải phẫu này phải mạnh mẽ bắt đầu từ mức độ của động mạch thận do nguy cơ di chuyển xuống xa trên độ cong ngoài và khó khăn trong việc định vị lại lên trên. Các tác giả đề xuất một kỹ thuật tiêu chuẩn với phương pháp tiếp cận nách-đùi xuyên suốt thông thường, sử dụng dây dẫn mềm cùng với việc đặt ống thông dự phòng động mạch thận dưới nếu có cổ ngắn (<15 mm). KẾT QUẢ Các thủ thuật nội mạch cho động mạch chủ đã được hoàn thành thành công ở tất cả các bệnh nhân. Việc triển khai cuối cùng của stent ghép bằng phương pháp kỹ thuật chuẩn hóa của chúng tôi là cực kỳ chính xác trong tất cả các trường hợp ngay cả khi cần phải triển khai lại ghép trong tất cả các trường hợp. Không cần bất kỳ thủ thuật thứ cấp nào khác. Vào ngày thứ 30, không ghi nhận rò rỉ nội mạc loại IA nào và không thực hiện bất kỳ thủ thuật thứ cấp nào liên quan đến phình động mạch. Thời gian theo dõi trung bình cho nhóm bệnh nhân này là 5,2 tháng (phạm vi 1-11). Kết quả ban đầu cho thấy không có rò rỉ nội mạc loại IA và không di chuyển tại chụp cắt lớp mạch máu. Không cần bất kỳ thủ thuật thứ cấp nào liên quan đến phình động mạch. KẾT LUẬN Sử dụng thường quy dây dẫn nách-đùi xuyên suốt kết hợp với đặt ống thông trước chọn lọc động mạch thận dưới cho phép triển khai chính xác ghép nội mạc Gore Excluder Conformable trong các giải phẫu khó có thể ảnh hưởng đến kết quả sớm."} +{"idx": 1870164, "en": "The case refers to a 51-year-old symptomatic man with a new SARS-CoV-2 RNA positive nasopharyngeal swab after two negative ones and the lack of significant development of antibody response measured by different diagnostic serological test. Our case underlines that a discrepancy between clinical course of SARS-CoV-2 infection and results from diagnostic tests may exist. This concept is rapidly emerging and supports the need for a deep knowledge of available and \"in development\" tests for a correct interpretation of their findings.", "gemini": "Trường hợp này đề cập đến một người đàn ông có triệu chứng 51 tuổi với xét nghiệm tăm bông mũi họng dương tính với RNA SARS-CoV-2 mới sau hai lần xét nghiệm âm tính và không có sự phát triển đáng kể về đáp ứng kháng thể được đo bằng xét nghiệm huyết thanh chẩn đoán khác nhau. Trường hợp của chúng tôi nhấn mạnh rằng có thể có sự khác biệt giữa diễn biến lâm sàng của nhiễm SARS-CoV-2 và kết quả từ các xét nghiệm chẩn đoán. Khái niệm này đang nổi lên nhanh chóng và hỗ trợ cho nhu cầu hiểu biết sâu sắc về các xét nghiệm có sẵn và \"đang phát triển\" để diễn giải chính xác các phát hiện của họ."} +{"idx": 1870165, "en": "INTRODUCTION COVID-19 has changed the approach to operating on breast cancer for the benefit of patients, staff and the general population. One approach involves the switch from operating under general to local anaesthetic. We assess whether diluational local anaesthetic is as effective as the current standard approach. METHODS Postoperative pain was recorded in prospective, consecutive patients undergoing wide local excision under dilutional local anaesthetic (concentration < 1mg/ml). Pain scores were documented at 0, 30 and 60 minutes and compared to a control group consisting of combined general with local anaesthetic. RESULTS Pain significantly increased in the control group during the postoperative recovery. This was not seen in the dilutional local anaesthetic group that was non-inferior to the standard approach at 0, 30 and 60 minutes. CONCLUSION Dilutional local anaesthetic provides a safe and effective alternative approach to operating on breast cancer patients whilst avoiding risky general anaesthetic in a COVID-19 pandemic environment.", "gemini": "GIỚI THIỆU COVID-19 đã làm thay đổi cách tiếp cận phẫu thuật ung thư vú để mang lại lợi ích cho bệnh nhân, nhân viên và cộng đồng nói chung. Một cách tiếp cận liên quan đến việc chuyển từ gây mê toàn thân sang gây tê tại chỗ. Chúng tôi đánh giá xem thuốc gây tê tại chỗ pha loãng có hiệu quả như cách tiếp cận tiêu chuẩn hiện tại hay không. PHƯƠNG PHÁP Đau sau phẫu thuật được ghi nhận ở những bệnh nhân liên tiếp, có triển vọng đang trải qua cắt bỏ tại chỗ rộng rãi dưới gây tê tại chỗ pha loãng (nồng độ <1mg/ml). Điểm đau được ghi lại ở phút 0, 30 và 60 và so sánh với nhóm đối chứng bao gồm gây mê toàn thân kết hợp với gây tê tại chỗ. KẾT QUẢ Đau tăng đáng kể ở nhóm đối chứng trong quá trình hồi phục sau phẫu thuật. Điều này không thấy ở nhóm gây tê tại chỗ pha loãng không kém gì cách tiếp cận tiêu chuẩn ở phút 0, 30 và 60. KẾT LUẬN Thuốc gây tê tại chỗ pha loãng cung cấp một cách tiếp cận thay thế an toàn và hiệu quả để phẫu thuật cho bệnh nhân ung thư vú trong khi tránh gây mê toàn thân có rủi ro trong môi trường đại dịch COVID-19."} +{"idx": 1870166, "en": "Visceral artery pseudoaneurysms are common complications of pancreatitis that carry a high mortality rate. Early detection and treatment of the pseudoaneurysms should be done as they carry high mortality following rupture. The splenic artery is the commonest artery involved followed by gastroduodenal and pancreaticoduodenal arteries. Gastric artery pseudoaneurysms are less common with less than 50 cases reported in the literature till now. We describe a case of gastric artery pseudoaneurysm secondary to chronic pancreatitis in a patient who presented with intermittent GI bleeding and also describe the role of endovascular management in the treatment of the same.", "gemini": "Phình động mạch giả nội tạng là biến chứng phổ biến của viêm tụy có tỷ lệ tử vong cao. Cần phát hiện và điều trị sớm các phình động mạch giả vì chúng có tỷ lệ tử vong cao sau khi vỡ. Động mạch lách là động mạch phổ biến nhất bị ảnh hưởng, tiếp theo là động mạch dạ dày tá tràng và tụy tá tràng. Phình động mạch giả động mạch dạ dày ít phổ biến hơn với chưa đến 50 trường hợp được báo cáo trong tài liệu cho đến nay. Chúng tôi mô tả một trường hợp phình động mạch giả động mạch dạ dày thứ phát do viêm tụy mạn tính ở một bệnh nhân biểu hiện chảy máu tiêu hóa từng cơn và cũng mô tả vai trò của quản lý nội mạch trong điều trị trường hợp này."} +{"idx": 1870167, "en": "Occupational exposure in spraying and application of non-arsenical insecticides has been classified as a probable human carcinogen. The fundamental molecular mechanisms involved the tumor-related genes. This study aimed to investigate the carcinogenesis effects related to chronic exposure to organophosphate (OP) pesticides in pesticide applicators. This was a cross-sectional study conducted on 27 pesticide applicators and 24 matched controls through the period from June to December 2018. The level of acetylcholinesterase (AChE) was determined and the effects of OPs exposure on messenger RNA (mRNA) expression of the DNA-damage responsive genes P53, P21, GADD45a, and MDM2 were determined using real-time quantitative polymerase chain reaction. A significant reduction of serum AChE enzyme activities was observed in chronically exposed subjects in comparison with the control group (p = 0.001). The expression of P53, P21 mRNA was significantly downregulated in the exposed group compared with the healthy nonexposed control group (p < 0.05). Conversely, the expression of MDM2 and GADD45a did not significantly differ between the exposed subjects and the control group (p > 0.05). No significant differences were noted between the exposed and control groups regarding the genotype or allele distributions of P53 Arg72Pro polymorphism. These results suggested that chronic exposure to OP insecticides may have mitogenic and carcinogenicity activity for the exposed cases due to downregulation of P53 and P21 but did not demonstrate any DNA damage properties for the exposed cases, and finally, a regular follow-up of the exposed cases for tumor markers is recommended.", "gemini": "Tiếp xúc nghề nghiệp khi phun và sử dụng thuốc trừ sâu không chứa asen đã được phân loại là tác nhân gây ung thư ở người có thể xảy ra. Các cơ chế phân tử cơ bản liên quan đến các gen liên quan đến khối u. Nghiên cứu này nhằm mục đích điều tra các tác động gây ung thư liên quan đến phơi nhiễm mạn tính với thuốc trừ sâu organophosphate (OP) ở những người phun thuốc trừ sâu. Đây là một nghiên cứu cắt ngang được tiến hành trên 27 người phun thuốc trừ sâu và 24 người đối chứng được ghép nối trong giai đoạn từ tháng 6 đến tháng 12 năm 2018. Nồng độ acetylcholinesterase (AChE) được xác định và các tác động của phơi nhiễm OP lên biểu hiện RNA thông tin (mRNA) của các gen phản ứng với tổn thương DNA P53, P21, GADD45a và MDM2 đã được xác định bằng phản ứng chuỗi polymerase định lượng thời gian thực. Hoạt động của enzym AChE trong huyết thanh giảm đáng kể ở những đối tượng tiếp xúc mạn tính so với nhóm đối chứng (p = 0,001). Sự biểu hiện của P53, P21 mRNA giảm đáng kể ở nhóm tiếp xúc so với nhóm đối chứng không tiếp xúc khỏe mạnh (p < 0,05). Ngược lại, sự biểu hiện của MDM2 và GADD45a không khác biệt đáng kể giữa những người tiếp xúc và nhóm đối chứng (p > 0,05). Không có sự khác biệt đáng kể nào được ghi nhận giữa các nhóm tiếp xúc và đối chứng về kiểu gen hoặc phân bố alen của đa hình P53 Arg72Pro. Những kết quả này cho thấy rằng tiếp xúc mạn tính với thuốc trừ sâu OP có thể có hoạt tính gây ung thư và gây ung thư cho các trường hợp tiếp xúc do sự điều hòa giảm P53 và P21 nhưng không biểu hiện bất kỳ đặc tính tổn thương DNA nào đối với các trường hợp tiếp xúc và cuối cùng, nên theo dõi thường xuyên các trường hợp tiếp xúc đối với các dấu ấn khối u."} +{"idx": 1870168, "en": "The #MeToo and Time's Up movements have sparked a significant cultural shift in the United States around sexual harassment and abuse by creating an environment of support, rather than one that punishes and silences women and men who come forward to tell their stories about abuse of all kinds. The Cape Verdean community faces a variety of complex challenges and barriers in addressing intimate partner violence. This article chronicles my experiences reporting the study findings from the Cape Verdean Women's Project (CVWP), which included Cape Verdean women's perceptions of intimate violence and their recommendations for social change.", "gemini": "Các phong trào #MeToo và Time's Up đã tạo nên một sự thay đổi văn hóa đáng kể tại Hoa Kỳ về vấn đề quấy rối và lạm dụng tình dục bằng cách tạo ra một môi trường hỗ trợ, thay vì một môi trường trừng phạt và làm im tiếng những người phụ nữ và nam giới lên tiếng kể về câu chuyện của họ về mọi hình thức lạm dụng. Cộng đồng Cape Verde phải đối mặt với nhiều thách thức và rào cản phức tạp trong việc giải quyết bạo lực của bạn tình. Bài viết này ghi lại kinh nghiệm của tôi khi báo cáo các phát hiện nghiên cứu từ Dự án Phụ nữ Cape Verde (CVWP), bao gồm nhận thức của phụ nữ Cape Verde về bạo lực gia đình và các khuyến nghị của họ về thay đổi xã hội."} +{"idx": 1870169, "en": "This article examines the experience of eight graduate students in the drama therapy program at Lesley University when creating and performing a theater piece centered around gender-based violence. The performance piece, A Space to Speak, used the performers' real-life stories to highlight their vastly different, yet strikingly similar, experiences and invited the audience to examine their own relationship to those stories. A description of the process used to create and perform the piece is followed by a discussion of the impact the process had on the performers and audience members.", "gemini": "Bài viết này xem xét kinh nghiệm của tám sinh viên tốt nghiệp trong chương trình trị liệu bằng kịch tại Đại học Lesley khi tạo và biểu diễn một vở kịch xoay quanh chủ đề bạo lực trên cơ sở giới. Vở diễn, A Space to Speak, sử dụng những câu chuyện có thật của người biểu diễn để làm nổi bật những trải nghiệm rất khác nhau nhưng lại vô cùng giống nhau của họ và mời khán giả xem xét mối quan hệ của chính họ với những câu chuyện đó. Sau phần mô tả về quá trình tạo và biểu diễn vở kịch là phần thảo luận về tác động của quá trình này đối với người biểu diễn và khán giả."} +{"idx": 1870170, "en": "The poems selected here explore the hidden side of some of the most popular fairy tales. Girls become women guided by myths that define their passive role, their submission to violent behavior by men in their lives, be they fathers, husbands, or lovers. These poems look at a girl's fantasies from a different perspective: that a woman's life becomes the work of demystifying the myths that dominated her childhood to create a newly informed and subversive version of the myth.", "gemini": "Những bài thơ được chọn ở đây khám phá mặt khuất của một số câu chuyện cổ tích phổ biến nhất. Các cô gái trở thành phụ nữ được dẫn dắt bởi những câu chuyện thần thoại định nghĩa vai trò thụ động của họ, sự khuất phục của họ trước hành vi bạo lực của những người đàn ông trong cuộc đời họ, dù là cha, chồng hay người yêu. Những bài thơ này nhìn vào tưởng tượng của một cô gái từ một góc độ khác: rằng cuộc đời của một người phụ nữ trở thành công việc phá bỏ những câu chuyện thần thoại thống trị tuổi thơ của cô ấy để tạo ra một phiên bản mới được thông tin và mang tính lật đổ của câu chuyện thần thoại đó."} +{"idx": 1870171, "en": "Nagaland has witnessed violent conflict for over five decades. It is a heavily militarized space where draconian laws like the Armed Forces Special Powers Act allow army personnel to go unchallenged even after committing violent crimes. Few women have used their tradition-specific gendered role strategically to subvert gender norms and exhibit agency against violence within the conflict situation and the systemic violence that bars them from entering the public-political sphere. This article studies how women from the Naga tribal communities use their tradition-specific gender roles of motherhood to gain agency and resist the formation of a hostile, gendered social space.", "gemini": "Nagaland đã chứng kiến cuộc xung đột bạo lực trong hơn năm thập kỷ. Đây là một không gian quân sự hóa nghiêm trọng, nơi các luật hà khắc như Đạo luật Quyền lực đặc biệt của Lực lượng vũ trang cho phép quân nhân không bị truy cứu ngay cả sau khi phạm tội bạo lực. Một số ít phụ nữ đã sử dụng vai trò giới tính theo truyền thống của họ một cách chiến lược để lật đổ các chuẩn mực giới và thể hiện sự chủ động chống lại bạo lực trong tình huống xung đột và bạo lực có hệ thống ngăn cản họ bước vào lĩnh vực công-chính trị. Bài viết này nghiên cứu cách phụ nữ từ các cộng đồng bộ lạc Naga sử dụng vai trò giới tính theo truyền thống của họ là người mẹ để có được sự chủ động và chống lại sự hình thành một không gian xã hội thù địch và có giới tính."} +{"idx": 1870172, "en": "I think what unites the given poems is a desire to interrogate oppressive language used as an attempt to regulate the power/humanity of or to control women, particularly Black and Brown women, myself included. This speech comes in many forms: whether in a reckless and racist tweet we see coming from the current U.S. President, a catcall on the street, a little ole micro-aggressive comment in the workplace, or in the instances taken up by the poems.", "gemini": "Tôi nghĩ rằng điểm chung của những bài thơ được đưa ra là mong muốn thẩm vấn ngôn ngữ áp bức được sử dụng như một nỗ lực để điều chỉnh quyền lực/nhân tính của phụ nữ hoặc kiểm soát phụ nữ, đặc biệt là phụ nữ Da đen và Da nâu, kể cả tôi. Bài phát biểu này xuất hiện dưới nhiều hình thức: có thể là một dòng tweet vô trách nhiệm và phân biệt chủng tộc mà chúng ta thấy đến từ Tổng thống Hoa Kỳ hiện tại, một tiếng huýt sáo trên phố, một bình luận nhỏ mang tính vi mô tại nơi làm việc hoặc trong các trường hợp mà các bài thơ nêu ra."} +{"idx": 1870173, "en": "The importance of cultural processes to behavioural diversity in our closest living relatives is central to revealing the evolutionary origins of human culture. However, the bonobo is often overlooked as a candidate model. Further, a prominent critique to many examples of proposed animal cultures is premature exclusion of environmental confounds known to shape behavioural phenotypes. We addressed these gaps by investigating variation in prey preference between neighbouring bonobo groups that associate and overlap space use. We find group preference for duiker or anomalure hunting otherwise unexplained by variation in spatial usage, seasonality, or hunting party size, composition, and cohesion. Our findings demonstrate that group-specific behaviours emerge independently of the local ecology, indicating that hunting techniques in bonobos may be culturally transmitted. The tolerant intergroup relations of bonobos offer an ideal context to explore drivers of behavioural phenotypes, the essential investigations for phylogenetic constructs of the evolutionary origins of culture.", "gemini": "Tầm quan trọng của các quá trình văn hóa đối với sự đa dạng về hành vi ở những người họ hàng gần nhất của chúng ta là trọng tâm để khám phá nguồn gốc tiến hóa của văn hóa loài người. Tuy nhiên, loài bonobo thường bị bỏ qua như một mô hình ứng viên. Hơn nữa, một lời chỉ trích nổi bật đối với nhiều ví dụ về các nền văn hóa động vật được đề xuất là loại trừ sớm các yếu tố gây nhiễu về môi trường được biết là định hình kiểu hình hành vi. Chúng tôi giải quyết những khoảng trống này bằng cách điều tra sự khác biệt về sở thích con mồi giữa các nhóm bonobo lân cận liên kết và chồng chéo sử dụng không gian. Chúng tôi tìm thấy sở thích của nhóm đối với việc săn bắt duiker hoặc anomalure nếu không thì không thể giải thích được bằng sự khác biệt về cách sử dụng không gian, tính theo mùa hoặc quy mô, thành phần và sự gắn kết của nhóm săn bắt. Những phát hiện của chúng tôi chứng minh rằng các hành vi cụ thể của nhóm xuất hiện độc lập với hệ sinh thái địa phương, chỉ ra rằng các kỹ thuật săn bắt ở loài bonobo có thể được truyền tải theo văn hóa. Mối quan hệ giữa các nhóm khoan dung của loài bonobo tạo ra một bối cảnh lý tưởng để khám phá các động lực của kiểu hình hành vi, những cuộc điều tra thiết yếu để xây dựng phả hệ về nguồn gốc tiến hóa của văn hóa."} +{"idx": 1870174, "en": "The evolutionary forces shaping life history divergence within species are largely unknown. Turquoise killifish display differences in lifespan among wild populations, representing an ideal natural experiment in evolution and diversification of life history. By combining genome sequencing and population genetics, we investigate the evolutionary forces shaping lifespan among wild turquoise killifish populations. We generate an improved reference genome assembly and identify genes under positive and purifying selection, as well as those evolving neutrally. Short-lived populations from the outer margin of the species range have small population size and accumulate deleterious mutations in genes significantly enriched in the WNT signaling pathway, neurodegeneration, cancer and the mTOR pathway. We propose that limited population size due to habitat fragmentation and repeated population bottlenecks, by increasing the genome-wide mutation load, exacerbates the effects of mutation accumulation and cumulatively contribute to the short adult lifespan.", "gemini": "Các lực tiến hóa định hình sự phân kỳ lịch sử sự sống trong các loài phần lớn vẫn chưa được biết đến. Cá sóc két màu ngọc lam thể hiện sự khác biệt về tuổi thọ giữa các quần thể hoang dã, đại diện cho một thí nghiệm tự nhiên lý tưởng về sự tiến hóa và đa dạng hóa lịch sử sự sống. Bằng cách kết hợp giải trình tự bộ gen và di truyền quần thể, chúng tôi nghiên cứu các lực tiến hóa định hình tuổi thọ giữa các quần thể cá sóc két màu ngọc lam hoang dã. Chúng tôi tạo ra một bộ gen tham chiếu được cải tiến và xác định các gen chịu chọn lọc dương tính và thanh lọc, cũng như các gen tiến hóa trung tính. Các quần thể có tuổi thọ ngắn từ rìa ngoài của phạm vi loài có quy mô quần thể nhỏ và tích lũy các đột biến có hại trong các gen được làm giàu đáng kể trong con đường truyền tín hiệu WNT, thoái hóa thần kinh, ung thư và con đường mTOR. Chúng tôi đề xuất rằng quy mô quần thể hạn chế do mất môi trường sống và sự thắt cổ chai của quần thể lặp đi lặp lại, bằng cách làm tăng tải đột biến trên toàn bộ hệ gen, làm trầm trọng thêm tác động của sự tích tụ đột biến và tích lũy góp phần vào tuổi thọ trưởng thành ngắn."} +{"idx": 1870175, "en": "New evidence that neighboring communities of bonobos hunt different prey species, despite extensive overlaps in where they live and hunt, is difficult to explain without invoking cultural factors.", "gemini": "Bằng chứng mới cho thấy các cộng đồng bonobo lân cận săn các loài con mồi khác nhau, mặc dù có sự chồng chéo rộng rãi về nơi chúng sống và săn mồi, rất khó giải thích nếu không viện dẫn đến các yếu tố văn hóa."} +{"idx": 1870176, "en": "Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters, which contains both cotransporters and exchangers. While DASS proteins transport substrates via an elevator mechanism, to date structures are only available for a single DASS cotransporter protein in a substrate-bound, inward-facing state. We report multiple cryo-EM and X-ray structures in four different states, including three hitherto unseen states, along with molecular dynamics simulations, of both a cotransporter and an exchanger. Comparison of these outward- and inward-facing structures reveal how the transport domain translates and rotates within the framework of the scaffold domain through the transport cycle. Additionally, we propose that DASS transporters ensure substrate coupling by a charge-compensation mechanism, and by structural changes upon substrate release.", "gemini": "Citrate, α-ketoglutarate và succinate là các chất trung gian của chu trình TCA cũng đóng vai trò thiết yếu trong tín hiệu trao đổi chất và điều hòa tế bào. Các di- và tricarboxylate này được vận chuyển vào tế bào bởi họ vận chuyển màng tế bào chất vận chuyển đồng vận anion hai hóa trị (DASS), chứa cả đồng vận chuyển và trao đổi. Trong khi các protein DASS vận chuyển chất nền thông qua cơ chế thang máy, cho đến nay các cấu trúc chỉ có sẵn cho một protein đồng vận chuyển DASS ở trạng thái liên kết chất nền, hướng vào trong. Chúng tôi báo cáo nhiều cấu trúc cryo-EM và X-quang ở bốn trạng thái khác nhau, bao gồm ba trạng thái chưa từng thấy cho đến nay, cùng với các mô phỏng động lực học phân tử, của cả đồng vận chuyển và trao đổi. So sánh các cấu trúc hướng ra ngoài và hướng vào này cho thấy cách miền vận chuyển dịch chuyển và xoay trong khuôn khổ của miền khung thông qua chu trình vận chuyển. Ngoài ra, chúng tôi đề xuất rằng các chất vận chuyển DASS đảm bảo liên kết chất nền bằng cơ chế bù điện tích và bằng các thay đổi cấu trúc khi giải phóng chất nền."} +{"idx": 1870177, "en": "APC/C-mediated proteolysis of cyclin B and securin promotes anaphase entry, inactivating CDK1 and permitting chromosome segregation, respectively. Reduction of CDK1 activity relieves inhibition of the CDK1-counteracting phosphatases PP1 and PP2A-B55, allowing wide-spread dephosphorylation of substrates. Meanwhile, continued APC/C activity promotes proteolysis of other mitotic regulators. Together, these activities orchestrate a complex series of events during mitotic exit. However, the relative importance of regulated proteolysis and dephosphorylation in dictating the order and timing of these events remains unclear. Using high temporal-resolution proteomics, we compare the relative extent of proteolysis and protein dephosphorylation. This reveals highly-selective rapid proteolysis of cyclin B, securin and geminin at the metaphase-anaphase transition, followed by slow proteolysis of other substrates. Dephosphorylation requires APC/C-dependent destruction of cyclin B and was resolved into PP1-dependent categories with unique sequence motifs. We conclude that dephosphorylation initiated by selective proteolysis of cyclin B drives the bulk of changes observed during mitotic exit.", "gemini": "Sự phân giải protein do APC/C trung gian của cyclin B và securin thúc đẩy sự xâm nhập vào kỳ giữa, lần lượt vô hiệu hóa CDK1 và cho phép phân tách nhiễm sắc thể. Giảm hoạt động của CDK1 làm giảm sự ức chế của các phosphatase đối kháng CDK1 là PP1 và PP2A-B55, cho phép khử phosphoryl hóa rộng rãi các chất nền. Trong khi đó, hoạt động liên tục của APC/C thúc đẩy sự phân giải protein của các chất điều hòa nguyên phân khác. Cùng nhau, các hoạt động này sắp xếp một loạt các sự kiện phức tạp trong quá trình thoát khỏi nguyên phân. Tuy nhiên, tầm quan trọng tương đối của sự phân giải protein được điều chỉnh và khử phosphoryl hóa trong việc quyết định thứ tự và thời gian của các sự kiện này vẫn chưa rõ ràng. Sử dụng proteomics có độ phân giải thời gian cao, chúng tôi so sánh mức độ tương đối của sự phân giải protein và khử phosphoryl hóa protein. Điều này cho thấy sự phân giải protein nhanh chóng và có chọn lọc cao của cyclin B, securin và geminin ở giai đoạn chuyển tiếp giữa kỳ giữa và kỳ sau, tiếp theo là sự phân giải protein chậm của các chất nền khác. Sự khử phosphoryl hóa đòi hỏi sự phá hủy cyclin B phụ thuộc APC/C và được giải quyết thành các loại phụ thuộc PP1 với các mô típ trình tự duy nhất. Chúng tôi kết luận rằng sự khử phosphoryl hóa được khởi đầu bởi sự phân giải protein có chọn lọc của cyclin B thúc đẩy phần lớn các thay đổi được quan sát thấy trong quá trình thoát khỏi nguyên phân."} +{"idx": 1870178, "en": "The basolateral amygdala complex (BLA), extensively connected with both local amygdalar nuclei as well as long-range circuits, is involved in a diverse array of functional roles. Understanding the mechanisms of such functional diversity will be greatly informed by understanding the cell-type-specific landscape of the BLA. Here, beginning with single-cell RNA sequencing, we identified both discrete and graded continuous gene-expression differences within the mouse BLA. Via in situ hybridization, we next mapped this discrete transcriptomic heterogeneity onto a sharp spatial border between the basal and lateral amygdala nuclei, and identified continuous spatial gene-expression gradients within each of these regions. These discrete and continuous spatial transformations of transcriptomic cell-type identity were recapitulated by local morphology as well as long-range connectivity. Thus, BLA excitatory neurons are a highly heterogenous collection of neurons that spatially covary in molecular, cellular, and circuit properties. This heterogeneity likely drives pronounced spatial variation in BLA computation and function.", "gemini": "Phức hợp hạnh nhân nền bên (BLA), kết nối rộng rãi với cả các nhân hạnh nhân tại chỗ cũng như các mạch dài, tham gia vào một loạt các vai trò chức năng đa dạng. Việc hiểu được các cơ chế của sự đa dạng chức năng như vậy sẽ được thông báo rất nhiều bằng cách hiểu được cảnh quan cụ thể của loại tế bào của BLA. Ở đây, bắt đầu với giải trình tự RNA tế bào đơn, chúng tôi đã xác định được cả sự khác biệt về biểu hiện gen liên tục rời rạc và được phân loại trong BLA của chuột. Thông qua lai tại chỗ, tiếp theo chúng tôi lập bản đồ tính không đồng nhất của bản sao transcriptomic rời rạc này lên một ranh giới không gian sắc nét giữa các nhân hạnh nhân nền và bên, và xác định các gradient biểu hiện gen không gian liên tục trong mỗi vùng này. Những biến đổi không gian rời rạc và liên tục này về bản sắc loại tế bào transcriptomic được lặp lại bởi hình thái cục bộ cũng như kết nối tầm xa. Do đó, các tế bào thần kinh kích thích BLA là một tập hợp các tế bào thần kinh rất không đồng nhất, có sự thay đổi về mặt không gian trong các đặc tính phân tử, tế bào và mạch. Sự không đồng nhất này có khả năng thúc đẩy sự thay đổi rõ rệt về mặt không gian trong tính toán và chức năng của BLA."} +{"idx": 1870179, "en": "The Polycomb repressive complex 2 (PRC2) is a multicomponent histone H3K27 methyltransferase complex, best known for silencing the Hox genes during embryonic development. The Polycomb-like proteins PHF1, MTF2, and PHF19 are critical components of PRC2 by stimulating its catalytic activity in embryonic stem cells. The Tudor domains of PHF1/19 have been previously shown to be readers of H3K36me3 in vitro. However, some other studies suggest that PHF1 and PHF19 co-localize with the H3K27me3 mark but not H3K36me3 in cells. Here, we provide further evidence that PHF1 co-localizes with H3t in testis and its Tudor domain preferentially binds to H3tK27me3 over canonical H3K27me3 in vitro. Our complex structures of the Tudor domains of PHF1 and PHF19 with H3tK27me3 shed light on the molecular basis for preferential recognition of H3tK27me3 by PHF1 and PHF19 over canonical H3K27me3, implicating that H3tK27me3 might be a physiological ligand of PHF1/19.", "gemini": "Phức hợp ức chế Polycomb 2 (PRC2) là một phức hợp methyltransferase histone H3K27 đa thành phần, được biết đến nhiều nhất với khả năng làm im lặng các gen Hox trong quá trình phát triển phôi. Các protein giống Polycomb PHF1, MTF2 và PHF19 là các thành phần quan trọng của PRC2 bằng cách kích thích hoạt động xúc tác của nó trong các tế bào gốc phôi. Các miền Tudor của PHF1/19 trước đây đã được chứng minh là các đầu đọc của H3K36me3 trong ống nghiệm. Tuy nhiên, một số nghiên cứu khác cho thấy PHF1 và PHF19 đồng định vị với dấu hiệu H3K27me3 nhưng không phải H3K36me3 trong tế bào. Ở đây, chúng tôi cung cấp thêm bằng chứng cho thấy PHF1 đồng định vị với H3t trong tinh hoàn và miền Tudor của nó liên kết ưu tiên với H3tK27me3 hơn H3K27me3 chuẩn trong ống nghiệm. Các cấu trúc phức tạp của các miền Tudor của PHF1 và PHF19 với H3tK27me3 làm sáng tỏ cơ sở phân tử cho sự nhận dạng ưu tiên của H3tK27me3 bởi PHF1 và PHF19 so với H3K27me3 chuẩn, ngụ ý rằng H3tK27me3 có thể là một phối tử sinh lý của PHF1/19."} +{"idx": 1870180, "en": "Perception depends on a complex interplay between feedforward and recurrent processing. Yet, while the former has been extensively characterized, the computational organization of the latter remains largely unknown. Here, we use magneto-encephalography to localize, track and decode the feedforward and recurrent processes of reading, as elicited by letters and digits whose level of ambiguity was parametrically manipulated. We first confirm that a feedforward response propagates through the ventral and dorsal pathways within the first 200 ms. The subsequent activity is distributed across temporal, parietal and prefrontal cortices, which sequentially generate five levels of representations culminating in action-specific motor signals. Our decoding analyses reveal that both the content and the timing of these brain responses are best explained by a hierarchy of recurrent neural assemblies, which both maintain and broadcast increasingly rich representations. Together, these results show how recurrent processes generate, over extended time periods, a cascade of decisions that ultimately accounts for subjects' perceptual reports and reaction times.", "gemini": "Thị giác phụ thuộc vào sự tương tác phức tạp giữa quá trình xử lý tiến tiếp và hồi tiếp. Tuy nhiên, trong khi quá trình trước đã được mô tả chi tiết, thì tổ chức tính toán của quá trình sau vẫn chưa được biết đến nhiều. Ở đây, chúng tôi sử dụng từ não đồ để định vị, theo dõi và giải mã các quá trình tiến tiếp và hồi tiếp của việc đọc, như được gợi ra bởi các chữ cái và chữ số có mức độ mơ hồ được thao tác theo tham số. Đầu tiên, chúng tôi xác nhận rằng phản ứng tiến tiếp lan truyền qua các đường dẫn bụng và lưng trong vòng 200 ms đầu tiên. Hoạt động tiếp theo được phân bố trên vỏ não thái dương, đỉnh và trước trán, tạo ra năm cấp độ biểu diễn tuần tự lên đến các tín hiệu vận động cụ thể. Các phân tích giải mã của chúng tôi tiết lộ rằng cả nội dung và thời gian của các phản ứng não này được giải thích tốt nhất bởi một hệ thống phân cấp các nhóm tế bào thần kinh hồi tiếp, cả hai đều duy trì và phát sóng các biểu diễn ngày càng phong phú. Cùng nhau, những kết quả này cho thấy các quá trình hồi tiếp tạo ra như thế nào, trong các khoảng thời gian kéo dài, một loạt các quyết định cuối cùng giải thích cho các báo cáo nhận thức và thời gian phản ứng của đối tượng."} +{"idx": 1870181, "en": "Astrocytes respond to and regulate neuronal activity, yet their role in mammalian behavior remains incompletely understood. Especially unclear is whether, and if so how, astrocyte activity regulates contextual fear memory, the dysregulation of which leads to pathological fear-related disorders. We generated GFAP-ChR2-EYFP rats to allow the specific activation of astrocytes in vivo by optogenetics. We found that after memory acquisition within a temporal window, astrocyte activation disrupted memory consolidation and persistently decreased contextual but not cued fear memory accompanied by reduced fear-related anxiety behavior. In vivo microdialysis experiments showed astrocyte photoactivation increased extracellular ATP and adenosine concentrations. Intracerebral blockade of adenosine A1 receptors (A1Rs) reversed the attenuation of fear memory. Furthermore, intracerebral or intraperitoneal injection of A1R agonist mimicked the effects of astrocyte activation. Therefore, our findings provide a deeper understanding of the astrocyte-mediated regulation of fear memory and suggest a new and important therapeutic strategy against pathological fear-related disorders.", "gemini": "Các tế bào hình sao đáp ứng và điều chỉnh hoạt động của tế bào thần kinh, nhưng vai trò của chúng trong hành vi của động vật có vú vẫn chưa được hiểu đầy đủ. Đặc biệt không rõ liệu, và nếu có thì hoạt động của tế bào hình sao điều chỉnh trí nhớ sợ hãi theo ngữ cảnh như thế nào, sự mất điều hòa của trí nhớ này dẫn đến các rối loạn liên quan đến sợ hãi bệnh lý. Chúng tôi đã tạo ra những con chuột GFAP-ChR2-EYFP để cho phép kích hoạt cụ thể các tế bào hình sao trong cơ thể sống bằng quang di truyền học. Chúng tôi phát hiện ra rằng sau khi thu thập trí nhớ trong một cửa sổ thời gian, hoạt động của tế bào hình sao làm gián đoạn quá trình củng cố trí nhớ và liên tục làm giảm trí nhớ sợ hãi theo ngữ cảnh nhưng không phải là trí nhớ sợ hãi theo tín hiệu đi kèm với hành vi lo lắng liên quan đến sợ hãi giảm. Các thí nghiệm vi phân tích in vivo cho thấy hoạt động quang của tế bào hình sao làm tăng nồng độ ATP và adenosine ngoại bào. Sự phong tỏa thụ thể adenosine A1 (A1R) trong não đảo ngược sự suy giảm trí nhớ sợ hãi. Hơn nữa, tiêm chất chủ vận A1R vào não hoặc màng bụng bắt chước tác dụng của hoạt động tế bào hình sao. Do đó, những phát hiện của chúng tôi cung cấp hiểu biết sâu hơn về sự điều chỉnh trí nhớ sợ hãi thông qua tế bào hình sao và gợi ý một chiến lược điều trị mới và quan trọng chống lại các rối loạn liên quan đến sợ hãi bệnh lý."} +{"idx": 1870182, "en": "Over the last decade, the performance of benchtop x-ray fluorescence computed tomography (XFCT) systems has been significantly enhanced through hardware and software optimizations. Recent studies have indicated the need of energy-resolving pixelated/array detectors in the x-ray detection component to further improve the sensitivity and image resolution of benchtop XFCT systems while meeting the realistic constraints of dose and scan time. Thus, it is of immediate interest in the research community to conduct the following investigations: (a) delineation of strengths/weaknesses of detection configurations that incorporate pixelated/array detectors in combination with two most frequently used (parallel-hole and pinhole) collimators; (b) one-to-one comparison of their performance under identical imaging conditions of benchtop XFCT. In this study, we developed a Geant4-based Monte Carlo model to investigate the effects of the aforementioned detection configurations on the sensitivity and image resolution of a benchtop XFCT system. Using this model, we simulated the detection of x-ray fluorescence and scattered photons from gold nanoparticle-containing phantoms using energy-resolving pixelated detectors coupled with parallel-hole and pinhole collimators. Simulation results demonstrated that the detector consisting of large pixels (1 mm × 1 mm) combined with a parallel-hole collimator had better sensitivity (i.e. lower detection limit) than the detector made of smaller pixels (0.25 mm × 0.25 mm) coupled with a pinhole collimator. In comparison, although slightly less sensitive, the latter detector configuration achieved better image resolution than did the former. Thus, a detection configuration consisting of a pixelated detector with submillimeter pixels and a pinhole collimator is preferable when image resolution is critical for benchtop XFCT applications. On the other hand, the detector with larger pixels coupled with a parallel-hole collimator is better suited for benchtop XFCT applications in which higher sensitivity and shorter scan time are essential.", "gemini": "Trong thập kỷ qua, hiệu suất của các hệ thống chụp cắt lớp huỳnh quang tia X trên mặt bàn (XFCT) đã được cải thiện đáng kể thông qua tối ưu hóa phần cứng và phần mềm. Các nghiên cứu gần đây đã chỉ ra nhu cầu về các máy dò mảng/pixel phân giải năng lượng trong thành phần phát hiện tia X để cải thiện hơn nữa độ nhạy và độ phân giải hình ảnh của các hệ thống XFCT trên mặt bàn trong khi đáp ứng các ràng buộc thực tế về liều lượng và thời gian quét. Do đó, cộng đồng nghiên cứu rất quan tâm đến việc tiến hành các cuộc điều tra sau: (a) mô tả điểm mạnh/điểm yếu của các cấu hình phát hiện kết hợp các máy dò mảng/pixel với hai bộ chuẩn trực được sử dụng thường xuyên nhất (lỗ song song và lỗ kim); (b) so sánh từng loại hiệu suất của chúng trong các điều kiện hình ảnh giống hệt nhau của XFCT trên mặt bàn. Trong nghiên cứu này, chúng tôi đã phát triển một mô hình Monte Carlo dựa trên Geant4 để điều tra các tác động của các cấu hình phát hiện đã đề cập ở trên đối với độ nhạy và độ phân giải hình ảnh của hệ thống XFCT trên mặt bàn. Sử dụng mô hình này, chúng tôi đã mô phỏng việc phát hiện huỳnh quang tia X và các photon tán xạ từ các hình nộm chứa hạt nano vàng bằng cách sử dụng các máy dò pixel phân giải năng lượng kết hợp với bộ chuẩn trực lỗ song song và lỗ kim. Kết quả mô phỏng chứng minh rằng máy dò bao gồm các pixel lớn (1 mm × 1 mm) kết hợp với bộ chuẩn trực lỗ song song có độ nhạy tốt hơn (tức là giới hạn phát hiện thấp hơn) so với máy dò được tạo thành từ các pixel nhỏ hơn (0,25 mm × 0,25 mm) kết hợp với bộ chuẩn trực lỗ kim. So sánh, mặc dù độ nhạy kém hơn một chút, nhưng cấu hình máy dò sau đạt được độ phân giải hình ảnh tốt hơn so với cấu hình trước. Do đó, cấu hình phát hiện bao gồm máy dò pixel với các pixel dưới milimét và bộ chuẩn trực lỗ kim được ưu tiên khi độ phân giải hình ảnh rất quan trọng đối với các ứng dụng XFCT trên mặt bàn. Mặt khác, máy dò có pixel lớn hơn kết hợp với bộ chuẩn trực lỗ song song phù hợp hơn cho các ứng dụng XFCT trên mặt bàn trong đó độ nhạy cao hơn và thời gian quét ngắn hơn là điều cần thiết."} +{"idx": 1870183, "en": "Fiber-coupled scintillation dosimeters are a cost-effective alternative to the conventional ion chambers in radiation dosimetry. However, stem effects from optical fibers such as Cerenkov radiation incur significant errors in the readout signal. Here we introduce a second near-infrared window dosimeter, dubbed as NIR2D, that can potentially be used as real-time radiation detector for clinical megavoltage beams. Lanthanide-based rare-earth NaYF4 nano-phosphors doped with both erbium and cerium elements were synthesized, and a compact 3D printed reader device integrated with a photodetector and data acquisition system was designed. The performance of the NIR2D was tested using a pre-clinical orthovoltage radiation source and a clinical megavoltage radiation source. The system was tested for dose linearity (100, 200, 600 MU), dose rate dependency (100, 200, 400, 600 MU min-1), and energy dependency (6, 10, 15 MV). Test results with the clinical linear accelerator demonstrated excellent dose linearity and dose rate independency when exposed to 6 MV linac beams-both data follows a linear trendline with R2 > 0.99. On the other hand, the NIR2D was energy dependent, where the readout dropped by 9% between 6 and 15 MV. For stem effects, we observed a finite Cerenkov contribution of 1%-3% when exposed between 100-600 MU min-1 (6 MV) and 3%-6% when exposed between 5-15 MV (600 MU min-1). While the stem effects were still observable, we expect that enhancing the current optical setup will simultaneously improve the scintillation signal and reduce the stem effects.", "gemini": "Máy đo liều tia sán lấp ghép sợi quang là một giải pháp thay thế tiết kiệm chi phí cho buồng ion thông thường trong phép đo liều bức xạ. Tuy nhiên, hiệu ứng gốc từ các sợi quang như bức xạ Cerenkov gây ra lỗi đáng kể trong tín hiệu đọc ra. Tại đây, chúng tôi giới thiệu một máy đo liều cửa sổ hồng ngoại gần thứ hai, được gọi là NIR2D, có khả năng được sử dụng làm máy dò bức xạ thời gian thực cho các chùm tia megavoltage lâm sàng. Các nano-phốt pho đất hiếm NaYF4 có gốc lanthanide được pha tạp với cả nguyên tố erbi và xeri đã được tổng hợp, và một thiết bị đọc in 3D nhỏ gọn tích hợp với máy dò ảnh và hệ thống thu thập dữ liệu đã được thiết kế. Hiệu suất của NIR2D đã được thử nghiệm bằng nguồn bức xạ điện áp thấp tiền lâm sàng và nguồn bức xạ điện áp cao lâm sàng. Hệ thống đã được thử nghiệm về tính tuyến tính liều (100, 200, 600 MU), phụ thuộc tốc độ liều (100, 200, 400, 600 MU min-1) và phụ thuộc năng lượng (6, 10, 15 MV). Kết quả thử nghiệm với máy gia tốc tuyến tính lâm sàng cho thấy tính tuyến tính liều tuyệt vời và không phụ thuộc tốc độ liều khi tiếp xúc với chùm tia linac 6 MV - cả hai dữ liệu đều theo đường xu hướng tuyến tính với R2> 0,99. Mặt khác, NIR2D phụ thuộc năng lượng, trong đó số đọc giảm 9% giữa 6 và 15 MV. Đối với hiệu ứng gốc, chúng tôi quan sát thấy đóng góp hữu hạn của Cerenkov là 1% -3% khi tiếp xúc trong khoảng 100-600 MU min-1 (6 MV) và 3% -6% khi tiếp xúc trong khoảng 5-15 MV (600 MU min-1). Mặc dù hiệu ứng gốc vẫn có thể quan sát được, chúng tôi hy vọng rằng việc cải thiện thiết lập quang học hiện tại sẽ đồng thời cải thiện tín hiệu phát quang và giảm hiệu ứng gốc."} +{"idx": 1870184, "en": "Tilings based on the cut-and-project method are key model systems for the description of aperiodic solids. Typically, quantities of interest in crystallography involve averaging over large patches, and are well defined only in the infinite-volume limit. In particular, this is the case for autocorrelation and diffraction measures. For cut-and-project systems, the averaging can conveniently be transferred to internal space, which means dealing with the corresponding windows. In this topical review, this is illustrated by the example of averaged shelling numbers for the Fibonacci tiling, and the standard approach to the diffraction for this example is recapitulated. Further, recent developments are discussed for cut-and-project structures with an inflation symmetry, which are based on an internal counterpart of the renormalization cocycle. Finally, a brief review is given of the notion of hyperuniformity, which has recently gained popularity, and its application to aperiodic structures.", "gemini": "Các phép ghép dựa trên phương pháp cắt và chiếu là các hệ thống mô hình chính để mô tả các chất rắn phi chu kỳ. Thông thường, các đại lượng quan tâm trong tinh thể học liên quan đến việc tính trung bình trên các miếng vá lớn và chỉ được xác định rõ trong giới hạn thể tích vô hạn. Đặc biệt, đây là trường hợp đối với các biện pháp tự tương quan và nhiễu xạ. Đối với các hệ thống cắt và chiếu, việc tính trung bình có thể được chuyển thuận tiện sang không gian bên trong, có nghĩa là xử lý các cửa sổ tương ứng. Trong bài tổng quan chuyên đề này, điều này được minh họa bằng ví dụ về số lượng vỏ trung bình cho phép ghép Fibonacci và phương pháp tiếp cận chuẩn đối với phép nhiễu xạ cho ví dụ này được tóm tắt lại. Ngoài ra, các phát triển gần đây được thảo luận đối với các cấu trúc cắt và chiếu có đối xứng giãn nở, dựa trên đối tác bên trong của chu kỳ chuẩn hóa. Cuối cùng, một bài tổng quan ngắn gọn được đưa ra về khái niệm siêu đồng nhất, gần đây đã trở nên phổ biến và ứng dụng của nó vào các cấu trúc phi chu kỳ."} +{"idx": 1870185, "en": "The basic idea of frozen-density embedding theory (FDET) is the constrained minimization of the Hohenberg-Kohn density functional EHK[ρ] performed using the auxiliary functional E_{v_{AB}}^{\\rm FDET}[\\Psi _A, \\rho _B], where ΨA is the embedded NA-electron wavefunction and ρB(r) is a non-negative function in real space integrating to a given number of electrons NB. This choice of independent variables in the total energy functional E_{v_{AB}}^{\\rm FDET}[\\Psi _A, \\rho _B] makes it possible to treat the corresponding two components of the total density using different methods in multi-level simulations. The application of FDET using ρB(r) reconstructed from X-ray diffraction data for a molecular crystal is demonstrated for the first time. For eight hydrogen-bonded clusters involving a chromophore (represented as ΨA) and the glycylglycine molecule [represented as ρB(r)], FDET is used to derive excitation energies. It is shown that experimental densities are suitable for use as ρB(r) in FDET-based simulations.", "gemini": "Ý tưởng cơ bản của lý thuyết nhúng mật độ đông lạnh (FDET) là giảm thiểu ràng buộc của hàm mật độ Hohenberg-Kohn EHK[ρ] được thực hiện bằng cách sử dụng hàm bổ trợ E_{v_{AB}}^{\\rm FDET}[\\Psi _A, \\rho _B], trong đó ΨA là hàm sóng NA electron được nhúng và ρB(r) là một hàm không âm trong không gian thực tích hợp với một số electron nhất định NB. Lựa chọn các biến độc lập này trong hàm tổng năng lượng E_{v_{AB}}^{\\rm FDET}[\\Psi _A, \\rho _B] giúp có thể xử lý hai thành phần tương ứng của tổng mật độ bằng các phương pháp khác nhau trong mô phỏng đa cấp. Việc áp dụng FDET sử dụng ρB(r) tái tạo từ dữ liệu nhiễu xạ tia X cho tinh thể phân tử được chứng minh lần đầu tiên. Đối với tám cụm liên kết hydro liên quan đến một nhóm sắc tố (được biểu diễn là ΨA) và phân tử glycylglycine [được biểu diễn là ρB(r)], FDET được sử dụng để tính năng lượng kích thích. Kết quả cho thấy mật độ thực nghiệm phù hợp để sử dụng làm ρB(r) trong các mô phỏng dựa trên FDET."} +{"idx": 1870186, "en": "The famous Euler's rule for three-dimensional polyhedra, F - E + V = 2 (F, E and V are the numbers of faces, edges and vertices, respectively), when extended to many tested cases of space-filling polyhedra such as the asymmetric unit (ASU), takes the form Fn - En + Vn = 1, where Fn, En and Vn enumerate the corresponding elements, normalized by their multiplicity, i.e. by the number of times they are repeated by the space-group symmetry. This modified formula holds for the ASUs of all 230 space groups and 17 two-dimensional planar groups as specified in the International Tables for Crystallography, and for a number of tested Dirichlet domains, suggesting that it may have a general character. The modification of the formula stems from the fact that in a symmetrical space-filling arrangement the polyhedra (such as the ASU) have incomplete bounding elements (faces, edges, vertices), since they are shared (in various degrees) with the space-filling neighbors.", "gemini": "Quy tắc Euler nổi tiếng cho các đa diện ba chiều, F - E + V = 2 (F, E và V lần lượt là số mặt, cạnh và đỉnh), khi mở rộng cho nhiều trường hợp đã được kiểm tra của các đa diện lấp đầy không gian như đơn vị bất đối xứng (ASU), có dạng Fn - En + Vn = 1, trong đó Fn, En và Vn liệt kê các phần tử tương ứng, được chuẩn hóa theo bội số của chúng, tức là theo số lần chúng được lặp lại bởi đối xứng nhóm không gian. Công thức đã sửa đổi này áp dụng cho các ASU của tất cả 230 nhóm không gian và 17 nhóm mặt phẳng hai chiều như được chỉ định trong Bảng quốc tế về tinh thể học và cho một số miền Dirichlet đã được kiểm tra, cho thấy rằng nó có thể có đặc tính chung. Việc sửa đổi công thức bắt nguồn từ thực tế là trong một sự sắp xếp lấp đầy không gian đối xứng, các đa diện (chẳng hạn như ASU) có các phần tử giới hạn không đầy đủ (mặt, cạnh, đỉnh), vì chúng được chia sẻ (ở các mức độ khác nhau) với các phần tử lấp đầy không gian. hàng xóm."} +{"idx": 1870187, "en": "The generating sets of {\\bb Z}^4 have been enumerated which consist of integral four-dimensional vectors with components -1, 0, 1 and allow Cayley graphs without edge intersections in a straight-edge embedding in a four-dimensional Euclidean space. Owing to computational restrictions the valency of enumerated graphs has been fixed to 10. Up to isomorphism 58 graphs have been found and characterized by coordination sequences, shortest cycles and automorphism groups. To compute automorphism groups, a novel strategy is introduced that is based on determining vertex stabilizers from the automorphism group of a sufficiently large finite ball cut out from an infinite graph. Six exceptional, rather `dense' graphs have been identified which are locally isomorphic to a five-dimensional cubic lattice within a ball of radius 10. They could be built by either interconnecting interpenetrated three- or four-dimensional cubic lattices and therefore necessarily contain Hopf links between quadrangular cycles. As a consequence, a local combinatorial isomorphism does not extend to a local isotopy.", "gemini": "Các tập hợp sinh của {\\bb Z} ^ 4 đã được liệt kê bao gồm các vectơ bốn chiều nguyên với các thành phần -1, 0, 1 và cho phép đồ thị Cayley không có giao điểm cạnh trong nhúng cạnh thẳng trong không gian Euclid bốn chiều. Do hạn chế về tính toán, hóa trị của đồ thị được liệt kê đã được cố định thành 10. Có tới 58 đồ thị đồng cấu đã được tìm thấy và được đặc trưng bởi các chuỗi phối hợp, chu trình ngắn nhất và nhóm tự đồng hình. Để tính các nhóm tự đồng hình, một chiến lược mới được giới thiệu dựa trên việc xác định các bộ ổn định đỉnh từ nhóm tự đồng hình của một quả bóng hữu hạn đủ lớn được cắt ra từ một đồ thị vô hạn. Sáu đồ thị đặc biệt, khá 'mật' đã được xác định, chúng đồng dạng cục bộ với mạng lập phương năm chiều trong một quả bóng bán kính 10. Chúng có thể được xây dựng bằng cách kết nối các mạng lập phương ba hoặc bốn chiều đan xen vào nhau và do đó nhất thiết phải chứa các liên kết Hopf giữa các chu trình tứ giác. Do đó, một phép đồng dạng tổ hợp cục bộ không mở rộng thành phép đồng vị cục bộ."} +{"idx": 1870188, "en": "Temperature-dependent total X-ray scattering measurements for water confined in bioactive glass samples with 5.9 nm pore diameter have been performed. Based on these experimental data, simulations were carried out using the Empirical Potential Structure Refinement (EPSR) code, in order to study the structural organization of the confined water in detail. The results indicate a non-homogeneous structure for water inside the pore, with three different structural organizations of water, depending on the distance from the pore surface: (i) a first layer (4 Å) of interfacial pore water that forms a strong chemical bond with the substrate, (ii) intermediate pore water forming a second layer (4-11 Å) on top of the interfacial pore water, (iii) bulk-like pore water in the centre of the pores. Analysis of the simulated site-site partial pair distribution function shows that the water-silica (Ow-Si) pair correlations occur at ∼3.75 Å. The tetrahedral network of bulk water with oxygen-oxygen (Ow-Ow) hydrogen-bonded pair correlations at ∼2.8, ∼4.1 and ∼4.5 Å is strongly distorted for the interfacial pore water while the second neighbour pair correlations are observed at ∼4.0 and ∼4.9 Å. For the interfacial pore water, an additional Ow-Ow pair correlation appears at ∼3.3 Å, which is likely caused by distortions due to the interactions of the water molecules with the silica at the pore surface.", "gemini": "Các phép đo tán xạ tia X tổng thể phụ thuộc nhiệt độ đối với nước bị giới hạn trong các mẫu thủy tinh hoạt tính sinh học có đường kính lỗ chân lông 5,9 nm đã được thực hiện. Dựa trên dữ liệu thực nghiệm này, các mô phỏng đã được thực hiện bằng cách sử dụng mã Empirical Potential Structure Refinement (EPSR), để nghiên cứu chi tiết về tổ chức cấu trúc của nước bị giới hạn. Kết quả chỉ ra một cấu trúc không đồng nhất đối với nước bên trong lỗ chân lông, với ba tổ chức cấu trúc khác nhau của nước, tùy thuộc vào khoảng cách từ bề mặt lỗ chân lông: (i) một lớp đầu tiên (4 Å) của nước lỗ chân lông giao diện tạo thành liên kết hóa học mạnh với chất nền, (ii) nước lỗ chân lông trung gian tạo thành lớp thứ hai (4-11 Å) trên đầu nước lỗ chân lông giao diện, (iii) nước lỗ chân lông giống như khối ở tâm của các lỗ chân lông. Phân tích hàm phân bố cặp một phần tại chỗ được mô phỏng cho thấy các tương quan cặp nước-silic (Ow-Si) xảy ra ở khoảng 3,75 Å. Mạng lưới tứ diện của nước khối với các tương quan cặp liên kết hydro oxy-oxy (Ow-Ow) ở khoảng 2,8, ∼4,1 và ∼4,5 Å bị biến dạng mạnh đối với nước lỗ chân lông giao diện trong khi các tương quan cặp lân cận thứ hai được quan sát ở khoảng 4,0 và ∼4,9 Å. Đối với nước lỗ chân lông giao diện, một tương quan cặp Ow-Ow bổ sung xuất hiện ở khoảng 3,3 Å, có thể là do các biến dạng do sự tương tác của các phân tử nước với silic ở bề mặt lỗ chân lông."} +{"idx": 1870189, "en": "Primitive substitution tilings on {\\bb R}^d whose expansion maps are unimodular are considered. It is assumed that all the eigenvalues of the expansion maps are algebraic conjugates with the same multiplicity. In this case, a cut-and-project scheme can be constructed with a Euclidean internal space. Under some additional condition, it is shown that if the substitution tiling has pure discrete spectrum, then the corresponding representative point sets are regular model sets in that cut-and-project scheme.", "gemini": "Nghiên cứu các phép lát thay thế nguyên thủy trên {\\bb R}^d có phép xạ ảnh mở rộng là đơn môđun. Giả sử rằng tất cả các giá trị riêng của phép xạ ảnh mở rộng là các liên hợp đại số có cùng bội số. Trong trường hợp này, có thể xây dựng một lược đồ cắt và chiếu với một không gian bên trong Euclid. Theo một số điều kiện bổ sung, nếu phép lát thay thế có phổ rời rạc thuần túy, thì các tập điểm đại diện tương ứng là các tập mô hình thông thường trong lược đồ cắt và chiếu đó."} +{"idx": 1870190, "en": "Mathematical knots and links are described as piecewise linear - straight, non-intersecting sticks meeting at corners. Isogonal structures have all corners related by symmetry (`vertex'-transitive). Corner- and stick-transitive structures are termed regular. No regular knots are found. Regular links are cubic or icosahedral and a complete account of these (36 in number) is given, including optimal (thickest-stick) embeddings. Stick 2-transitive isogonal structures are again cubic and icosahedral and also encompass the infinite family of torus knots and links. The major types of these structures are identified and reported with optimal embeddings. The relevance of this work to materials chemistry and biochemistry is noted.", "gemini": "Các nút và liên kết toán học được mô tả là các đoạn thẳng tuyến tính từng phần - thẳng, không giao nhau, gặp nhau ở các góc. Các cấu trúc đẳng góc có tất cả các góc liên quan đến nhau theo phép đối xứng (`đỉnh'-truyền). Các cấu trúc truyền góc và truyền đoạn thẳng được gọi là cấu trúc chính quy. Không tìm thấy nút chính quy nào. Các liên kết chính quy là lập phương hoặc hình hai mươi mặt và một mô tả đầy đủ về các liên kết này (có 36 liên kết) được đưa ra, bao gồm cả các nhúng tối ưu (đoạn thẳng dày nhất). Các cấu trúc đẳng góc truyền 2 đoạn thẳng một lần nữa là lập phương và hình hai mươi mặt và cũng bao gồm họ vô hạn các nút và liên kết hình xuyến. Các loại chính của các cấu trúc này được xác định và báo cáo với các nhúng tối ưu. Tính liên quan của công trình này đến hóa học vật liệu và hóa sinh được ghi nhận."} +{"idx": 1870191, "en": "In E6, the cone of positive definite quadratic forms is subdivided into Σs subcones and its equivalence classes {\\cal E}_{\\Sigma _s} are determined for s = 0-3, and 18-21.", "gemini": "Trong E6, hình nón của các dạng bậc hai xác định dương được chia thành Σs hình nón phụ và các lớp tương đương {\\cal E}_{\\Sigma _s} của nó được xác định cho s = 0-3 và 18-21."} +{"idx": 1870192, "en": "All possible combinatorial embeddings into primitive cubic networks of arbitrary tilings of 3D space by pairwise congruent and parallel regular hexagonal prisms are discussed and classified.", "gemini": "Tất cả các nhúng kết hợp có thể vào các mạng lập phương nguyên thủy của các lớp phủ tùy ý của không gian 3D bằng các lăng kính lục giác đều song song và trùng khít từng đôi một được thảo luận và phân loại."} +{"idx": 1870193, "en": "At the time of writing, the COVID-19 pandemic is assuming a distinct shape as do healthcare systems around the world. Some countries resisted to the tsunami and are now re-opening their industrial and commercial activities while re-organizing to face a possible new wave. Others are still struggling not to be overwhelmed by the most significant public health challenge of the last century. In Italy, after a strict lockdown, almost all activities are re-opening, trying to navigate between Scylla (epidemics and its economic consequences) and Cariddi (economic recession and its adverse health effects) bearing in mind that there is collinearity between the circulation of money and spreading of the virus and that there is a serious risk of a vicious spiral which could affect the society. The prolonged lockdown deemed to prevent the spreading of the virus also reduced the circulation of money, and hence tax revenues, thus it will ultimately result in fewer finances available for social security and Public Health (3). The main political issue will then be the definition of a right point of equilibrium between risks and benefits, between action and precaution. As scientists, we are called to distinguish between what we know and what is unknown, between data and opinions, between facts and beliefs. [...].", "gemini": "Tại thời điểm viết bài, đại dịch COVID-19 đang diễn ra theo một hình thái riêng biệt như các hệ thống chăm sóc sức khỏe trên toàn thế giới. Một số quốc gia đã chống lại cơn sóng thần và hiện đang mở lại các hoạt động công nghiệp và thương mại của mình trong khi tổ chức lại để đối mặt với một làn sóng mới có thể xảy ra. Những quốc gia khác vẫn đang vật lộn để không bị choáng ngợp trước thách thức về sức khỏe cộng đồng đáng kể nhất trong thế kỷ qua. Tại Ý, sau lệnh phong tỏa nghiêm ngặt, hầu hết các hoạt động đều được mở cửa trở lại, cố gắng điều hướng giữa Scylla (dịch bệnh và hậu quả kinh tế của nó) và Cariddi (suy thoái kinh tế và tác động xấu đến sức khỏe) khi lưu ý rằng có sự tương quan giữa lưu thông tiền tệ và sự lây lan của vi-rút và có nguy cơ nghiêm trọng của một vòng xoáy luẩn quẩn có thể ảnh hưởng đến xã hội. Việc phong tỏa kéo dài được coi là để ngăn chặn sự lây lan của vi-rút cũng làm giảm lưu thông tiền tệ và do đó là doanh thu thuế, do đó cuối cùng sẽ dẫn đến ít tiền hơn cho an sinh xã hội và Y tế công cộng (3). Vấn đề chính trị chính sau đó sẽ là xác định điểm cân bằng giữa rủi ro và lợi ích, giữa hành động và phòng ngừa. Là các nhà khoa học, chúng ta được kêu gọi phân biệt giữa những gì chúng ta biết và những gì chưa biết, giữa dữ liệu và ý kiến, giữa sự thật và niềm tin. [...]."} +{"idx": 1870194, "en": "BACKGROUND Criteria for diagnosis and compensation of occupational musculoskeletal diseases varies widely between countries as demonstrated by the large differences between countries with comparable economics and social systems (for example, within the European Union). Several countries have a list of occupational diseases and sometimes these lists include diagnostic and attribution criteria, but these criteria are usually not very specific, and they may also be very different. OBJECTIVES The aim of this paper is to explicitly define what are the information needed for an evidence-based diagnosis and attribution of an occupational musculoskeletal disease. METHODS Based on the general framework of evidence-based medicine, a review is presented of the information required to define: - when a musculoskeletal disease is present, according to the best available techniques; - how to define a relevant exposure to biomechanical risk factors, according to the best available techniques. RESULTS Criteria are presented to combine information regarding the diagnosis of a musculoskeletal disease and exposure to biomechanical risk factors for an evidence-based attribution of the disease to the occupational exposure. The criteria use a probabilistic model that combine epidemiologic and medical findings, workplace exposure assessment, and non-occupational factors evaluation. DISCUSSION The use of the proposed criteria may improve the process of diagnosis and attribution of an occupational musculoskeletal disease. In addition, it makes possible to associate a probability rank to the attribution and, ultimately, it may improve the overall quality of the decisional process of the occupational physician.", "gemini": "HOÀN CẢNH Các tiêu chí chẩn đoán và bồi thường cho các bệnh cơ xương nghề nghiệp khác nhau rất nhiều giữa các quốc gia như đã chứng minh bằng sự khác biệt lớn giữa các quốc gia có nền kinh tế và hệ thống xã hội tương đương (ví dụ: trong Liên minh Châu Âu). Một số quốc gia có danh sách các bệnh nghề nghiệp và đôi khi các danh sách này bao gồm các tiêu chí chẩn đoán và quy kết, nhưng các tiêu chí này thường không cụ thể lắm và cũng có thể rất khác nhau. MỤC TIÊU Mục đích của bài báo này là xác định rõ ràng thông tin cần thiết cho chẩn đoán và quy kết bệnh cơ xương nghề nghiệp dựa trên bằng chứng. PHƯƠNG PHÁP Dựa trên khuôn khổ chung của y học dựa trên bằng chứng, chúng tôi trình bày một đánh giá về thông tin cần thiết để xác định: - khi nào một bệnh cơ xương có mặt, theo các kỹ thuật tốt nhất hiện có; - cách xác định mức phơi nhiễm có liên quan đến các yếu tố rủi ro cơ sinh học, theo các kỹ thuật tốt nhất hiện có. KẾT QUẢ Các tiêu chí được trình bày để kết hợp thông tin liên quan đến chẩn đoán bệnh cơ xương và mức phơi nhiễm với các yếu tố rủi ro cơ sinh học để quy kết bệnh dựa trên bằng chứng cho phơi nhiễm nghề nghiệp. Các tiêu chí sử dụng một mô hình xác suất kết hợp các phát hiện dịch tễ học và y tế, đánh giá phơi nhiễm tại nơi làm việc và đánh giá các yếu tố không liên quan đến nghề nghiệp. THẢO LUẬN Việc sử dụng các tiêu chí được đề xuất có thể cải thiện quá trình chẩn đoán và quy kết bệnh cơ xương nghề nghiệp. Ngoài ra, nó giúp có thể liên kết một thứ hạng xác suất với quy kết và cuối cùng, nó có thể cải thiện chất lượng tổng thể của quá trình ra quyết định của bác sĩ chuyên khoa nghề nghiệp."} +{"idx": 1870195, "en": "BACKGROUND The eye is an important sensory organ occupationally exposed to ionizing radiation (IR) in healthcare workers (HCWs) engaged in medical imaging (MI). New evidence highlights the possible induction of cataract at IR exposure levels to be much lower than expected in the past. OBJECTIVE Conduct an updated review on the current evidence on cataract risk in healthcare workers exposed to IR. METHODS Published scientific studies on cataract risk in IR exposed healthcare workers were collected through a systematic search of two biomedical databases (MEDLINE and Scopus). Data from included studies was extracted and summarized. Study quality was also assessed. RESULTS All 21 eligible studies reported an increased prevalence of cataract, especially posterior subcapsular cataract, in IR exposed  HCWs with a higher prevalence in interventional cardiology staff. DISCUSSION Our review synthesizes the latest evidence to support the hypothesis of a significantly increased risk of occupational cataract in healthcare workers operating MI and exposed to IR, especially in interventional cardiologists. Data also support a dose-response relationship between IR exposure and the prevalence of opacities, especially posterior subcapsular opacities. CONCLUSIONS Findings highlight the need for effective control measures including appropriate training, adherence to protective procedures, and a constant use of shields and eye personal protective equipment in healthcare workers with optical exposure to IR. Periodic health surveillance programs, possibly including lens evaluation, are also important to monitor cataract risk in these MI operators.", "gemini": "BỐI CẢNH Mắt là cơ quan cảm giác quan trọng tiếp xúc với bức xạ ion hóa (IR) trong công việc của nhân viên y tế (HCW) tham gia vào chẩn đoán hình ảnh (MI). Bằng chứng mới nêu bật khả năng gây đục thủy tinh thể ở mức độ phơi nhiễm IR thấp hơn nhiều so với dự kiến trước đây. MỤC TIÊU Thực hiện đánh giá cập nhật về bằng chứng hiện tại về nguy cơ đục thủy tinh thể ở nhân viên y tế tiếp xúc với IR. PHƯƠNG PHÁP Các nghiên cứu khoa học đã công bố về nguy cơ đục thủy tinh thể ở nhân viên y tế tiếp xúc với IR đã được thu thập thông qua tìm kiếm có hệ thống từ hai cơ sở dữ liệu y sinh học (MEDLINE và Scopus). Dữ liệu từ các nghiên cứu có liên quan đã được trích xuất và tóm tắt. Chất lượng nghiên cứu cũng được đánh giá. KẾT QUẢ Tất cả 21 nghiên cứu đủ điều kiện đều báo cáo tỷ lệ mắc đục thủy tinh thể tăng, đặc biệt là đục thủy tinh thể dưới bao sau ở những nhân viên y tế tiếp xúc với IR, với tỷ lệ mắc cao hơn ở nhân viên can thiệp tim mạch. THẢO LUẬN Đánh giá của chúng tôi tổng hợp các bằng chứng mới nhất để hỗ trợ giả thuyết về nguy cơ đục thủy tinh thể do nghề nghiệp tăng đáng kể ở nhân viên y tế vận hành MI và tiếp xúc với IR, đặc biệt là ở bác sĩ tim mạch can thiệp. Dữ liệu cũng hỗ trợ mối quan hệ liều-phản ứng giữa phơi nhiễm IR và tỷ lệ mắc các độ mờ đục, đặc biệt là độ mờ đục dưới bao sau. KẾT LUẬN Các phát hiện nêu bật nhu cầu về các biện pháp kiểm soát hiệu quả bao gồm đào tạo thích hợp, tuân thủ các quy trình bảo vệ và sử dụng liên tục các tấm chắn và thiết bị bảo vệ mắt cá nhân ở những nhân viên y tế tiếp xúc với IR. Các chương trình giám sát sức khỏe định kỳ, có thể bao gồm cả đánh giá ống kính, cũng rất quan trọng để theo dõi nguy cơ đục thủy tinh thể ở những người vận hành MI này."} +{"idx": 1870196, "en": "BACKGROUND The construction industry is characterized by a high prevalence of accidents and injuries. Inadequate risk management measures, including failure to use or incorrect use of personal protective equipment (PPE) may significantly increase the risk of accidents. OBJECTIVES The main objectives of the current study were to measure the prevalence of PPE use and accidents and their associated factors among construction workers. METHODS A cross-sectional field study with an analytic component was carried out on 384 workers from different sites in Port-Said, Egypt, using an interview administered questionnaire. The questionnaire included sociodemographic and occupational data, practice of PPE use and accident analysis. RESULTS About 60% of workers use PPE during work. Main reasons for non-use are discomfort, lack of knowledge on how to use it and poor fit. Occupational accidents in the last 12 months were reported by 64.3% of workers. The main accident types were: being hit by falling objects, falls from height, and tool related accidents. Safety training was the significant independent predictor of PPE use (AOR=2.0). However, age, marital status, smoking, safety training, and PPE use were also significant independent predictors of accidents (AOR=2.4, 3.1, 0.5, 0.5, and 0.2; respectively). DISCUSSION Among construction workers, PPE utilization is low with significant relation to safety training while occupational accidents are common and significantly related to safety training and PPE use. Therefore, safety training should be provided, and PPE use should be enforced at construction sites.", "gemini": "BỐI CẢNH Ngành xây dựng được đặc trưng bởi tỷ lệ tai nạn và thương tích cao. Các biện pháp quản lý rủi ro không đầy đủ, bao gồm việc không sử dụng hoặc sử dụng không đúng cách các thiết bị bảo vệ cá nhân (PPE) có thể làm tăng đáng kể nguy cơ xảy ra tai nạn. MỤC TIÊU Các mục tiêu chính của nghiên cứu hiện tại là đo lường tỷ lệ sử dụng PPE và tai nạn cũng như các yếu tố liên quan của chúng trong số công nhân xây dựng. PHƯƠNG PHÁP Một nghiên cứu thực địa cắt ngang với thành phần phân tích đã được thực hiện trên 384 công nhân từ các địa điểm khác nhau ở Port-Said, Ai Cập, bằng cách sử dụng bảng câu hỏi phỏng vấn. Bảng câu hỏi bao gồm dữ liệu nhân khẩu học và nghề nghiệp, thực hành sử dụng PPE và phân tích tai nạn. KẾT QUẢ Khoảng 60% công nhân sử dụng PPE trong quá trình làm việc. Những lý do chính khiến không sử dụng là khó chịu, thiếu kiến thức về cách sử dụng và không vừa vặn. 64,3% công nhân báo cáo đã gặp tai nạn nghề nghiệp trong 12 tháng qua. Các loại tai nạn chính là: bị vật rơi trúng, ngã từ trên cao và tai nạn liên quan đến công cụ. Đào tạo an toàn là yếu tố dự báo độc lập quan trọng về việc sử dụng PPE (AOR = 2,0). Tuy nhiên, tuổi tác, tình trạng hôn nhân, hút thuốc, đào tạo an toàn và sử dụng PPE cũng là những yếu tố dự báo độc lập quan trọng về tai nạn (lần lượt là AOR = 2,4, 3,1, 0,5, 0,5 và 0,2). THẢO LUẬN Trong số những công nhân xây dựng, việc sử dụng PPE còn thấp và có mối quan hệ đáng kể với việc đào tạo an toàn trong khi tai nạn nghề nghiệp phổ biến và có liên quan đáng kể đến việc đào tạo an toàn và sử dụng PPE. Do đó, cần cung cấp đào tạo an toàn và thực thi việc sử dụng PPE tại các công trường xây dựng."} +{"idx": 1870197, "en": "BACKGROUND Occupational pesticide poisoning is an important public health concern worldwide, especially in developing countries. OBJECTIVES This study was aimed at determining the prevalence and risk factors of acute pesticide poisoning (APP) among the vegetable and fruit farmers in Karacabey District in northwest Turkey. METHODS The study group consisted of 565 farmers. The dependent variable of the study is APP defined according to the World Health Organization's APP case definition matrix. The study's independent variables are the farmers' sociodemographic characteristics and the preventive measures they use when they are applying pesticides. The chi-square and logistic regression analysis analyzed the relationship between the dependent and independent variables. RESULTS A total of 64 (11.3%) farmers reported APP and 75% of them presented to a health institution due to symptoms. The factors associated with increased risk of APP were: illiteracy (odds ratio (OR)=2.5), 14 years and less farming experience (OR=3.3), not reading the pesticide labels (OR=6.4), and contact with liquid pesticides (OR=2.3). -Conclusions: The study shows that approximately one out of ten farmers experience APP. Training programs should be planned and monitored to improve farmers' awareness of the dangers of pesticides, and they should be encouraged to adopt and implement protective measures.", "gemini": "BỐI CẢNH Ngộ độc thuốc trừ sâu do nghề nghiệp là mối quan tâm lớn về sức khỏe cộng đồng trên toàn thế giới, đặc biệt là ở các nước đang phát triển. MỤC TIÊU Nghiên cứu này nhằm xác định tỷ lệ mắc và các yếu tố nguy cơ ngộ độc thuốc trừ sâu cấp tính (APP) ở những người trồng rau và trái cây ở Quận Karacabey ở phía tây bắc Thổ Nhĩ Kỳ. PHƯƠNG PHÁP Nhóm nghiên cứu gồm 565 nông dân. Biến phụ thuộc của nghiên cứu là APP được định nghĩa theo ma trận định nghĩa trường hợp APP của Tổ chức Y tế Thế giới. Các biến độc lập của nghiên cứu là các đặc điểm nhân khẩu học và nghề nghiệp của người nông dân cũng như các biện pháp phòng ngừa mà họ sử dụng khi phun thuốc trừ sâu. Phân tích hồi quy logistic và chi bình phương đã phân tích mối quan hệ giữa các biến phụ thuộc và biến độc lập. KẾT QUẢ Tổng cộng có 64 (11,3%) nông dân báo cáo mắc APP và 75% trong số họ đến cơ sở y tế vì các triệu chứng. Các yếu tố liên quan đến nguy cơ mắc APP tăng lên là: mù chữ (tỷ lệ chênh lệch (OR) = 2,5), kinh nghiệm làm nông 14 năm trở xuống (OR = 3,3), không đọc nhãn thuốc trừ sâu (OR = 6,4) và tiếp xúc với thuốc trừ sâu dạng lỏng (OR = 2,3). - Kết luận: Nghiên cứu cho thấy cứ mười nông dân thì có khoảng một người bị APP. Các chương trình đào tạo nên được lập kế hoạch và theo dõi để nâng cao nhận thức của người nông dân về những nguy cơ của thuốc trừ sâu và họ nên được khuyến khích áp dụng và thực hiện các biện pháp bảo vệ."} +{"idx": 1870198, "en": "BACKGROUND A high level of organizational well-being improves employee performance and influences the physical and mental health of healthcare providers and students. OBJECTIVE This study investigates the relationship between the work context, organizational well-being, and the psychophysical health of healthcare providers. METHODS A multicentre cross-sectional descriptive study was conducted on a sample of healthcare providers (physicians and nurses) and healthcare students (medical students and nursing students). A self-report questionnaire was administered between September and November 2016. -Results: Of the 300 questionnaires administered, 201 (67%) were correctly completed. Overall, both the physical and mental health of the healthcare providers and students are explained by the variables of the organizational context: organizational well-being and socio-demographic/work characteristics. In particular, the results show a dependence on gender and age. Furthermore, decision latitude had a positive effect on physical health (b=.134) while job demands had a negative effect (b=-.160) explaining 21% of the PCS of the healthcare providers and students (R2=.209). Mental health improved via the satisfaction (b=.345), and positivity (b=.222) of healthcare professionals and students of these disciplines. DISCUSSION The results are significant because they directly impact the quality of care provided as well as patient safety.", "gemini": "BỐI CẢNH Mức độ hài lòng trong tổ chức cao sẽ cải thiện hiệu suất của nhân viên và ảnh hưởng đến sức khỏe thể chất và tinh thần của các nhà cung cấp dịch vụ chăm sóc sức khỏe và sinh viên. MỤC TIÊU Nghiên cứu này tìm hiểu mối quan hệ giữa bối cảnh công việc, sự hài lòng trong tổ chức và sức khỏe tâm sinh lý của các nhà cung cấp dịch vụ chăm sóc sức khỏe. PHƯƠNG PHÁP Một nghiên cứu mô tả cắt ngang đa trung tâm đã được tiến hành trên một mẫu các nhà cung cấp dịch vụ chăm sóc sức khỏe (bác sĩ và y tá) và sinh viên chăm sóc sức khỏe (sinh viên y khoa và sinh viên điều dưỡng). Một bảng câu hỏi tự báo cáo đã được tiến hành từ tháng 9 đến tháng 11 năm 2016. - Kết quả: Trong số 300 bảng câu hỏi được tiến hành, 201 (67%) được hoàn thành chính xác. Nhìn chung, cả sức khỏe thể chất và tinh thần của các nhà cung cấp dịch vụ chăm sóc sức khỏe và sinh viên đều được giải thích bằng các biến số của bối cảnh tổ chức: sự hài lòng trong tổ chức và đặc điểm nhân khẩu học/công việc. Đặc biệt, kết quả cho thấy sự phụ thuộc vào giới tính và tuổi tác. Hơn nữa, phạm vi quyết định có tác động tích cực đến sức khỏe thể chất (b = .134) trong khi nhu cầu công việc có tác động tiêu cực (b = -.160) giải thích 21% PCS của các nhà cung cấp dịch vụ chăm sóc sức khỏe và sinh viên (R2 = .209). Sức khỏe tinh thần được cải thiện thông qua sự hài lòng (b = .345) và sự tích cực (b = .222) của các chuyên gia chăm sóc sức khỏe và sinh viên của các chuyên ngành này. THẢO LUẬN Các kết quả có ý nghĩa vì chúng tác động trực tiếp đến chất lượng chăm sóc được cung cấp cũng như sự an toàn của bệnh nhân."} +{"idx": 1870199, "en": "Although Bernardino Ramazzini is usually regarded only as the father of occupational medicine, his approach to fight the devastating rinderpest epidemic in 1711 is worth mentioning in the light of the public health emergency represented by Coronavirus Disease-2019 (COVID-19). This commentary (i) focuses on Ramazzini's xiii oration describing that event, (ii) analyses the approach suggested to fight the cattle epidemic and economic threats, (iii) highlights some similar aspects between the 1711 rinderpest epidemic and the current COVID-19 pandemic (team expertise, contagion transmissibility, drug treatment, preventive measures, decision timeliness).", "gemini": "Mặc dù Bernardino Ramazzini thường chỉ được coi là cha đẻ của y học nghề nghiệp, nhưng cách tiếp cận của ông để chống lại đại dịch rinderpest tàn khốc vào năm 1711 rất đáng được nhắc đến trong bối cảnh tình trạng khẩn cấp về sức khỏe cộng đồng do Bệnh do virus Corona-2019 (COVID-19) gây ra. Bài bình luận này (i) tập trung vào bài diễn văn thứ xiii của Ramazzini mô tả sự kiện đó, (ii) phân tích cách tiếp cận được đề xuất để chống lại dịch bệnh gia súc và các mối đe dọa về kinh tế, (iii) nêu bật một số khía cạnh tương tự giữa dịch rinderpest năm 1711 và đại dịch COVID-19 hiện nay (chuyên môn của nhóm, khả năng lây truyền, điều trị bằng thuốc, các biện pháp phòng ngừa, tính kịp thời của quyết định)."} +{"idx": 1870200, "en": "The risk of transmission of COVID-19 or other serious respiratory infections during a pandemic event can dissuade lay rescuers from practicing resuscitation maneuvers; this may result in a reduction in efficacy in the treatment of the subject with cardiorespiratory arrest; this can also happen in occupational settings for the \"designed first aid worker\". In this letter we propose some considerations on the adaptation of the guidelines for cardiopulmonary resuscitation to pandemic events and on the protection of workers designated to first aid.", "gemini": "Nguy cơ lây truyền COVID-19 hoặc các bệnh nhiễm trùng đường hô hấp nghiêm trọng khác trong sự kiện đại dịch có thể ngăn cản những người cứu hộ không chuyên thực hiện các biện pháp hồi sức; điều này có thể dẫn đến việc giảm hiệu quả trong điều trị đối tượng bị ngừng tim hô hấp; điều này cũng có thể xảy ra trong môi trường nghề nghiệp đối với \"nhân viên sơ cứu được chỉ định\". Trong bức thư này, chúng tôi đề xuất một số cân nhắc về việc điều chỉnh các hướng dẫn về hồi sức tim phổi đối với các sự kiện đại dịch và về việc bảo vệ những người lao động được chỉ định sơ cứu."} +{"idx": 1870201, "en": "BACKGROUND The aim of this study was to explore the emotional resilience of middle school students learning at home in February and March 2020 during the COVID-19 pandemic, and the impact of this resilience on students' learning management skills. The results could provide a basis for psychological health education of middle school students during major life events. MATERIAL AND METHODS This study used a survey to explore emotional resilience and learning management abilities in middle school students (N=896) from February 10, 2020, to March 22, 2020. Students used online e-learning during this period due to the COVID-19 pandemic. Data were analyzed using t-testing, Pearson's correlation, and multivariate linear regression. RESULTS The results indicate that emotional resilience was significantly lower in eighth grade students than in seventh grade students (t=1.98, P<0.05) and negative emotional recovery increased during the study period. Emotional resilience was positively correlated with learning management skills (r=0.498, P<0.01), and positive emotional ability predicted learning management skills. CONCLUSIONS The study findings indicate that in the face of major life events, emotional resilience is an important factor for the mental health of adolescents and improves coping ability. Cultivating positive emotions can improve learning efficiency.", "gemini": "BỐI CẢNH Mục tiêu của nghiên cứu này là tìm hiểu khả năng phục hồi về mặt cảm xúc của học sinh trung học cơ sở học tại nhà vào tháng 2 và tháng 3 năm 2020 trong thời kỳ đại dịch COVID-19 và tác động của khả năng phục hồi này đối với các kỹ năng quản lý học tập của học sinh. Kết quả có thể cung cấp cơ sở cho giáo dục sức khỏe tâm lý của học sinh trung học cơ sở trong các sự kiện quan trọng của cuộc đời. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu này sử dụng một cuộc khảo sát để tìm hiểu khả năng phục hồi về mặt cảm xúc và khả năng quản lý học tập ở học sinh trung học cơ sở (N = 896) từ ngày 10 tháng 2 năm 2020 đến ngày 22 tháng 3 năm 2020. Học sinh sử dụng học trực tuyến trong thời gian này do đại dịch COVID-19. Dữ liệu được phân tích bằng cách kiểm tra t, tương quan Pearson và hồi quy tuyến tính đa biến. KẾT QUẢ Kết quả cho thấy khả năng phục hồi về mặt cảm xúc ở học sinh lớp 8 thấp hơn đáng kể so với học sinh lớp 7 (t = 1,98, P < 0,05) và khả năng phục hồi cảm xúc tiêu cực tăng lên trong thời gian nghiên cứu. Khả năng phục hồi về mặt cảm xúc có tương quan thuận với các kỹ năng quản lý học tập (r = 0,498, P < 0,01) và khả năng cảm xúc tích cực dự đoán các kỹ năng quản lý học tập. KẾT LUẬN Các phát hiện của nghiên cứu chỉ ra rằng khi đối mặt với các sự kiện quan trọng của cuộc đời, khả năng phục hồi về mặt cảm xúc là một yếu tố quan trọng đối với sức khỏe tinh thần của thanh thiếu niên và cải thiện khả năng đối phó. Nuôi dưỡng những cảm xúc tích cực có thể cải thiện hiệu quả học tập."} +{"idx": 1870202, "en": "Traditionally, plasma is extracted from whole blood using centrifuges in clinical laboratories, which is unsuitable for on-site testing. For point-of-care diagnostics, for example in HIV tests, to ensure the detection sensitivity for low-abundance analytical targets, a relatively large volume of plasma needs to be extracted from milliliters of blood with a simpler and easier-to-operate method than centrifugation. We report the development of a membrane-assisted, sedimentation-facilitated plasma separator with a multifunctional deformable chamber, which is able to perform plasma separation from undiluted whole blood in a short time. Multiple steps related to plasma separation, including cell sedimentation, cell filtration, and plasma driving and discharging, are all performed in or through the multifunctional deformable chamber equipped with a top-layer porous membrane, which significantly reduces the device complexity. Assisted by a simple jig or even hands, plasma separation can be conveniently performed upon mechanical actuation of the deformable chamber. Within 8 min, ∼130 μL of plasma can be conveniently extracted with the described device from 2.3 mL of whole blood. It has been demonstrated that HIV antibodies or virus spiked in whole blood can be successfully detected with reasonable sensitivity from the extracted plasma with the described pump-free device.", "gemini": "Theo truyền thống, huyết tương được chiết xuất từ máu toàn phần bằng máy ly tâm trong các phòng xét nghiệm lâm sàng, không phù hợp để xét nghiệm tại chỗ. Đối với chẩn đoán tại nơi chăm sóc, ví dụ như xét nghiệm HIV, để đảm bảo độ nhạy phát hiện đối với các mục tiêu phân tích có nồng độ thấp, cần chiết xuất một lượng huyết tương tương đối lớn từ nhiều mililít máu bằng phương pháp đơn giản hơn và dễ vận hành hơn so với phương pháp ly tâm. Chúng tôi báo cáo về sự phát triển của một bộ tách huyết tương hỗ trợ màng, tạo điều kiện lắng đọng với buồng biến dạng đa chức năng, có khả năng tách huyết tương khỏi máu toàn phần chưa pha loãng trong thời gian ngắn. Nhiều bước liên quan đến việc tách huyết tương, bao gồm lắng tế bào, lọc tế bào và dẫn và thải huyết tương, đều được thực hiện trong hoặc thông qua buồng biến dạng đa chức năng được trang bị màng xốp lớp trên cùng, giúp giảm đáng kể độ phức tạp của thiết bị. Được hỗ trợ bởi một dụng cụ đơn giản hoặc thậm chí bằng tay, việc tách huyết tương có thể được thực hiện thuận tiện khi kích hoạt cơ học buồng biến dạng. Trong vòng 8 phút, có thể chiết xuất thuận tiện khoảng 130 μL huyết tương bằng thiết bị được mô tả từ 2,3 mL máu toàn phần. Đã chứng minh rằng kháng thể HIV hoặc vi-rút tăng đột biến trong máu toàn phần có thể được phát hiện thành công với độ nhạy hợp lý từ huyết tương đã chiết xuất bằng thiết bị không cần bơm được mô tả."} +{"idx": 1870203, "en": "Optimisation of bioprocesses relies on approaches that are either labour intensive or require expensive robotic systems. There is a need for fluidic processing at low volume that can be integrated with existing bioprocess analytics to provide analytical information for the development and optimisation of bioprocesses. We demonstrate a 1 mL polymer inkjet 3D printed (i3DP) microbioreactor with integrated sensing (pH, oxygen and cell density) for optimisation of recombinant protein production with different feeds. A pressurised fluid driving system was used to control flow rates down to 0.7 μL min-1 with fluid switching from four reservoirs using a manifold controlled by solenoid valves. Oxygen transferred from a headspace via a gas-permeable membrane achieved a kLa of up to 90 h-1 at 1500 rpm. Cultivation of E. coli within the microbioreactor was comparable with a 2 L bench scale bioreactor, with optical densities of respectively 7.1 ± 0.4 and 6.5 ± 0.35. Triplicate batch cultivations within the microbioreactor of Pichia pastoris, with diauxic growth on glycerol (0.20 ± 0.02 h-1) and methanol (0.02 ± 0.04 h-1), showed good control of pH and DO and achieved a maximum dry cell weight of 10 ± 1 g L-1. For continuous cultivations, recombinant protein production was higher in pure methanol (314 ± 23) than methanol-sorbitol (202 ± 17) but reduces over time with lower cellular viability for methanol-glucose mixed feed, with less total protein produced and increases in DNA and proteases released. The developed system could be used in different applications including within synthetic biology, cell and gene therapy and organ-on-chips.", "gemini": "Tối ưu hóa các quy trình sinh học dựa trên các phương pháp đòi hỏi nhiều lao động hoặc đòi hỏi hệ thống rô bốt đắt tiền. Có nhu cầu xử lý chất lỏng ở thể tích thấp có thể tích hợp với phân tích quy trình sinh học hiện có để cung cấp thông tin phân tích cho việc phát triển và tối ưu hóa các quy trình sinh học. Chúng tôi trình bày một vi sinh học 3D được in bằng máy in phun polyme 1 mL (i3DP) với cảm biến tích hợp (pH, oxy và mật độ tế bào) để tối ưu hóa sản xuất protein tái tổ hợp với các nguồn cấp dữ liệu khác nhau. Một hệ thống dẫn chất lỏng có áp suất được sử dụng để kiểm soát tốc độ dòng xuống 0,7 μL phút -1 với việc chuyển chất lỏng từ bốn bình chứa sử dụng một ống phân phối được điều khiển bằng van điện từ. Oxy được truyền từ không gian đầu qua màng thấm khí đạt kLa lên đến 90 h-1 ở tốc độ 1500 vòng / phút. Nuôi cấy E. coli trong vi sinh học có thể so sánh với một lò phản ứng sinh học quy mô băng ghế 2 L, với mật độ quang học lần lượt là 7,1 ± 0,4 và 6,5 ± 0,35. Các đợt nuôi cấy mẻ ba lần trong vi sinh học của Pichia pastoris, với sự tăng trưởng diauxic trên glycerol (0,20 ± 0,02 h-1) và methanol (0,02 ± 0,04 h-1), cho thấy khả năng kiểm soát pH và DO tốt và đạt được trọng lượng tế bào khô tối đa là 10 ± 1 g L-1. Đối với các đợt nuôi cấy liên tục, sản xuất protein tái tổ hợp cao hơn ở methanol tinh khiết (314 ± 23) so với methanol-sorbitol (202 ± 17) nhưng giảm theo thời gian với khả năng sống tế bào thấp hơn đối với nguồn cấp hỗn hợp methanol-glucose, với lượng protein tổng thể được sản xuất ít hơn và tăng lượng DNA và protease được giải phóng. Hệ thống được phát triển có thể được sử dụng trong các ứng dụng khác nhau bao gồm sinh học tổng hợp, liệu pháp tế bào và gen và các cơ quan trên chip."} +{"idx": 1870204, "en": "The amount of copper affects the quality of herbal medicine greatly, it is necessary to develop some simple and sensitive methods to detect copper for the remote or resource-limited area. An electronic balance is one of the most familiar equipment that can be found nearly in all laboratories. The presence of Cu(i) can catalyze azide-alkyne cycloaddition reaction (called as click chemistry) with high efficiency. In this study, a simple method had been developed to detect copper ions in herbal medicine using an electronic balance as a readout device based on click chemistry. Cu(ii) is reduced to Cu(i) by sodium ascorbate in situ, which induces the \"click\" reaction between azido-DNA modified magnetic beads (MB-DNA) and alkynyl-DNA modified platinum nanoparticles (Pt NP-DNA) and results in the fixing of the platinum nanoparticles on the beads (called as MB-Pt NPs). MB-Pt NPs can be separated by a magnetic frame easily and transferred into a drainage reaction device containing hydrogen peroxide. Then, hydrogen peroxide can be decomposed by Pt NPs modified on MB to generate oxygen, which increases the pressure in the drainage reaction device and forces the water in the system to be discharged. The weight of the discharged water can be easily and accurately measured by an electronic balance. The weight of the water has a linear relationship with Cu(ii) in the range of 2.0-200 μM and a detection limit of 0.83 μM under 30 min of collected time. This method does not need complicated and expensive instruments, skilled technicians, and a complex data processing process. The proposed method had been applied to detect copper ions in herbal medicine with satisfactory results.", "gemini": "Lượng đồng ảnh hưởng rất lớn đến chất lượng của thuốc thảo dược, cần phải phát triển một số phương pháp đơn giản và nhạy để phát hiện đồng cho các vùng xa xôi hoặc thiếu nguồn lực. Cân điện tử là một trong những thiết bị quen thuộc nhất có thể tìm thấy ở hầu hết các phòng thí nghiệm. Sự có mặt của Cu(i) có thể xúc tác phản ứng cộng vòng azide-alkyne (gọi là phản ứng hóa học click) với hiệu suất cao. Trong nghiên cứu này, một phương pháp đơn giản đã được phát triển để phát hiện các ion đồng trong thuốc thảo dược bằng cách sử dụng cân điện tử làm thiết bị đọc dựa trên phản ứng hóa học click. Cu(ii) được khử thành Cu(i) bởi natri ascorbate tại chỗ, phản ứng này gây ra phản ứng \"click\" giữa các hạt từ tính đã được sửa đổi bằng azido-DNA (MB-DNA) và các hạt nano bạch kim đã được sửa đổi bằng alkynyl-DNA (Pt NP-DNA) và kết quả là cố định các hạt nano bạch kim trên các hạt (gọi là MB-Pt NPs). MB-Pt NPs có thể dễ dàng tách ra bằng khung từ và chuyển vào thiết bị phản ứng thoát nước chứa hydro peroxide. Sau đó, hydro peroxide có thể bị phân hủy bởi các hạt nano Pt được sửa đổi trên MB để tạo ra oxy, làm tăng áp suất trong thiết bị phản ứng thoát nước và buộc nước trong hệ thống phải thoát ra ngoài. Trọng lượng của nước thải ra có thể dễ dàng và chính xác được đo bằng cân điện tử. Trọng lượng của nước có mối quan hệ tuyến tính với Cu(ii) trong khoảng 2,0-200 μM và giới hạn phát hiện là 0,83 μM trong thời gian thu thập 30 phút. Phương pháp này không cần các dụng cụ phức tạp và đắt tiền, các kỹ thuật viên lành nghề và quá trình xử lý dữ liệu phức tạp. Phương pháp được đề xuất đã được áp dụng để phát hiện các ion đồng trong thuốc thảo dược với kết quả đạt yêu cầu."} +{"idx": 1870205, "en": "The selective reduction of esters to aldehydes, via the formation of stable alkyl silyl acetals, was, for the first time, achieved with both manganese, -Mn2(CO)10- and rhenium -Re2(CO)10- catalysts in the presence of triethylsilane as reductant. These two methods provide a direct access to a large variety of aliphatic and aromatic alkyl silyl acetals (30 examples) and to the corresponding aldehydes (13 examples) upon hydrolysis. The reactions proceeded in excellent yields and high selectivity at room temperature under photo-irradiation conditions (LED, 365 nm, 40 W, 9 h).", "gemini": "Việc khử este thành aldehyde một cách chọn lọc, thông qua sự hình thành các axetal silyl alkyl bền vững, lần đầu tiên đạt được với cả mangan, -Mn2(CO)10- và rheni -Re2(CO)10- chất xúc tác có sự hiện diện của trietylsilane như chất khử. Hai phương pháp này cung cấp một phương pháp tiếp cận trực tiếp đến nhiều loại axetal silyl alkyl aliphatic và thơm (30 ví dụ) và đến các aldehyde tương ứng (13 ví dụ) sau khi thủy phân. Các phản ứng diễn ra với hiệu suất tuyệt vời và độ chọn lọc cao ở nhiệt độ phòng trong điều kiện chiếu xạ ánh sáng (LED, 365 nm, 40 W, 9 giờ)."} +{"idx": 1870206, "en": "The simplest-level electron nuclear dynamics (SLEND) method with the coherent-states (CSs) quantum reconstruction procedure (CSQRP) is applied to the scattering system H+ + CO2 (000) → H+ + CO2 (v1v2v3) at ELab = 20.5-30 eV. Relevant for astrophysics, atmospheric chemistry and proton cancer therapy, this system undergoes collision-induced vibrational excitations in CO2. SLEND is a time-dependent, variational, direct, and non-adiabatic method that adopts a classical-mechanics description for nuclei and a single-determinantal wavefunction for electrons. The CSQRP employs the canonical CS to reconstruct quantum state-to-state vibrational properties from the SLEND classical nuclear dynamics. Overall, the calculated collision-induced vibrational properties agree well with experimental data. SLEND total differential cross sections (DCSs) agree remarkably well with their experimental counterparts and accurately display rainbow scattering angles structures. SLEND averaged target excitation energies for vibrational + rotational and rotational motions exhibit reasonable and good agreements with experimental data, respectively. These properties show that rotational excitation is low and that the asymmetric stretch normal mode of CO2 is much more excited than the others. SLEND/CSQRP state-to-state vibrational DCSs agree reasonably well with the sparse experimental data for final states v1v2v3 = 000-002, but less satisfactorily for 003. These DCSs also accurately display rainbow scattering angles structures. Finally, SLEND/CSQRP vibrational proton energy loss spectra agree remarkably well with their experimental counterparts for various final vibrational states of CO2, collisions energies and scattering angles. Present results demonstrate the accuracy of SLEND/CSQRP to predict state-to-state vibrational properties in scattering systems with multiple normal modes.", "gemini": "Phương pháp động lực học hạt nhân điện tử ở mức đơn giản nhất (SLEND) với quy trình tái tạo lượng tử trạng thái nhất quán (CSQRP) được áp dụng cho hệ thống tán xạ H+ + CO2 (000) → H+ + CO2 (v1v2v3) ở ELab = 20,5-30 eV. Có liên quan đến vật lý thiên văn, hóa học khí quyển và liệu pháp ung thư bằng proton, hệ thống này trải qua các kích thích rung động do va chạm trong CO2. SLEND là một phương pháp trực tiếp, không phiếm nhiệt, biến thiên theo thời gian, áp dụng mô tả cơ học cổ điển cho hạt nhân và hàm sóng xác định đơn cho electron. CSQRP sử dụng CS chuẩn tắc để tái tạo các tính chất rung động trạng thái lượng tử từ động lực học hạt nhân cổ điển SLEND. Nhìn chung, các tính chất rung động do va chạm được tính toán phù hợp với dữ liệu thực nghiệm. Các mặt cắt vi phân tổng SLEND (DCS) phù hợp đáng kể với các mặt cắt thực nghiệm và hiển thị chính xác các cấu trúc góc tán xạ cầu vồng. Các năng lượng kích thích mục tiêu trung bình của SLEND cho chuyển động rung + quay và quay lần lượt thể hiện sự đồng ý hợp lý và tốt với dữ liệu thực nghiệm. Các tính chất này cho thấy rằng sự kích thích quay thấp và chế độ bình thường kéo dài không đối xứng của CO2 được kích thích nhiều hơn nhiều so với các chế độ khác. Các DCS rung động trạng thái SLEND/CSQRP phù hợp hợp lý với dữ liệu thực nghiệm thưa thớt cho các trạng thái cuối v1v2v3 = 000-002, nhưng kém thỏa đáng hơn đối với 003. Các DCS này cũng hiển thị chính xác các cấu trúc góc tán xạ cầu vồng. Cuối cùng, phổ mất năng lượng proton rung động SLEND/CSQRP phù hợp đáng kể với các phổ thực nghiệm của chúng đối với các trạng thái rung động cuối cùng khác nhau của CO2, năng lượng va chạm và góc tán xạ. Các kết quả hiện tại chứng minh độ chính xác của SLEND/CSQRP để dự đoán các tính chất rung động trạng thái trong các hệ thống tán xạ với nhiều chế độ bình thường."} +{"idx": 1870207, "en": "Multi-electron, multi-proton transfer is important in a wide spectrum of processes spanning biological, chemical and physical systems. These reactions have attracted significant interest due to both fundamental curiosity and potential applications in energy technology. In this Perspective Review, we shed light on modern aspects of electrode processes in the 21st century, in particular on the recent advances and challenges in multistep electron/proton transfers at solid-liquid interfaces. Ongoing developments of analytical techniques and operando spectrometry at electrode/electrolyte interfaces and reliable computational approaches to simulate complicated interfacial electrochemical reactions enable us to obtain microscopic insights about these complex processes, such as the role of quantum effects in electrochemical reactions. Our motivation in this Perspective Review is to provide a comprehensive survey and discussion of state-of-the-art developments in experiments, materials, and theories for modern electrode process science, as well as to present an outlook for the future directions in this field.", "gemini": "Sự chuyển dịch nhiều electron, nhiều proton rất quan trọng trong một loạt các quá trình trải dài từ các hệ thống sinh học, hóa học đến vật lý. Các phản ứng này đã thu hút sự quan tâm đáng kể do cả sự tò mò cơ bản và các ứng dụng tiềm năng trong công nghệ năng lượng. Trong Đánh giá theo góc nhìn này, chúng tôi làm sáng tỏ các khía cạnh hiện đại của các quá trình điện cực trong thế kỷ 21, đặc biệt là những tiến bộ và thách thức gần đây trong quá trình chuyển electron/proton nhiều bước tại các giao diện rắn-lỏng. Sự phát triển liên tục của các kỹ thuật phân tích và phép đo quang phổ operando tại các giao diện điện cực/chất điện phân và các phương pháp tính toán đáng tin cậy để mô phỏng các phản ứng điện hóa giao diện phức tạp cho phép chúng tôi có được những hiểu biết sâu sắc về các quá trình phức tạp này, chẳng hạn như vai trò của hiệu ứng lượng tử trong các phản ứng điện hóa. Động lực của chúng tôi trong Đánh giá theo góc nhìn này là cung cấp một cuộc khảo sát toàn diện và thảo luận về những phát triển tiên tiến nhất trong các thí nghiệm, vật liệu và lý thuyết cho khoa học về quá trình điện cực hiện đại, cũng như đưa ra triển vọng cho các hướng đi trong tương lai trong lĩnh vực này."} +{"idx": 1870208, "en": "A novel low cost sodium-ion battery electrolyte additive ZSM-5 nanozeolite, which improves cycle life, is demonstrated in a Na-ion cell. The addition of this zeolitic small molecule scavenger removes electrolyte decomposition products, and has beneficial properties compared to traditional organic additives such as fluoroethylene carbonate (FEC). Capacity retention after 480 cycles improves from 40% (none) compared to 62% (ZSM-5). This is due to the enhanced interface stability over the cell life-time, as shown by XPS.", "gemini": "Một chất phụ gia điện phân pin ion natri giá rẻ mới, nanozeolit ZSM-5, giúp cải thiện tuổi thọ chu kỳ, được chứng minh trong một cell ion Na. Việc bổ sung chất loại bỏ phân tử nhỏ dạng zeolit này giúp loại bỏ các sản phẩm phân hủy điện phân và có các đặc tính có lợi hơn so với các chất phụ gia hữu cơ truyền thống như fluoroetylen cacbonat (FEC). Dung lượng duy trì sau 480 chu kỳ cải thiện từ 40% (không có) so với 62% (ZSM-5). Điều này là do sự ổn định của giao diện được tăng cường trong suốt thời gian sử dụng của cell, như được thể hiện bởi XPS."} +{"idx": 1870209, "en": "The presence of contaminants of emerging concern (CECs) in wastewater effluent and surface waters is an important field of research for analytical scientists. This study takes a suspect screening approach to wastewater and surface water analysis using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC-TOFMS). Two extraction procedures, traditional liquid-liquid extraction (LLE) and stir bar sorptive extraction (SBSE), were utilized and evaluated for their application to wastewater and surface water samples. Both techniques were evaluated regarding their recovery rates, range of compound classes extracted, and on their application to discovery of CECs. For the 14 surrogate compounds analyzed, LLE was able to extract all of them in each matrix with a recovery range of 19% to 159% and a median value of 74%. For SBSE, the recovery rates ranged from 19% to 117% with the median value at 66%, but only 8 of the compounds were able to be extracted because of the polarity bias for this extraction method. A new method of SBSE calibration was also developed using direct liquid injection of the internal standards before desorption of the stir bars. Initial findings indicate increased sensitivity and a greater range of unknown analyte recovery for SBSE, especially in the more dilute effluent and surface water samples. With the methods used in this study, SBSE has a concentration factor of approximately 416, improving that of LLE, which is 267. Suspect screening analysis was utilized to tentatively identify 32 CECs in the samples, the majority of which were pharmaceuticals and personal care products. More CECs were found using SBSE than LLE, especially in the surface water samples where 13 CECs were tentatively identified in the SBSE samples compared to 6 in the LLE samples.", "gemini": "Sự hiện diện của các chất gây ô nhiễm đáng lo ngại mới nổi (CEC) trong nước thải và nước mặt là một lĩnh vực nghiên cứu quan trọng đối với các nhà khoa học phân tích. Nghiên cứu này áp dụng phương pháp sàng lọc nghi ngờ đối với phân tích nước thải và nước mặt bằng cách sử dụng sắc ký khí hai chiều toàn diện với phép đo phổ khối thời gian bay (GC × GC-TOFMS). Hai quy trình chiết xuất, chiết xuất lỏng-lỏng truyền thống (LLE) và chiết xuất hấp phụ thanh khuấy (SBSE), đã được sử dụng và đánh giá để ứng dụng vào các mẫu nước thải và nước mặt. Cả hai kỹ thuật đều được đánh giá về tỷ lệ thu hồi, phạm vi các lớp hợp chất được chiết xuất và về ứng dụng của chúng để phát hiện CEC. Đối với 14 hợp chất thay thế được phân tích, LLE có thể chiết xuất tất cả chúng trong mỗi ma trận với tỷ lệ thu hồi từ 19% đến 159% và giá trị trung bình là 74%. Đối với SBSE, tỷ lệ thu hồi dao động từ 19% đến 117% với giá trị trung bình là 66%, nhưng chỉ có 8 hợp chất có thể được chiết xuất do độ phân cực của phương pháp chiết xuất này. Một phương pháp hiệu chuẩn SBSE mới cũng được phát triển bằng cách tiêm trực tiếp các tiêu chuẩn nội bộ trước khi giải hấp các thanh khuấy. Những phát hiện ban đầu chỉ ra độ nhạy tăng lên và phạm vi thu hồi chất phân tích chưa biết lớn hơn đối với SBSE, đặc biệt là trong các mẫu nước thải và nước mặt pha loãng hơn. Với các phương pháp được sử dụng trong nghiên cứu này, SBSE có hệ số cô đặc khoảng 416, cải thiện so với LLE là 267. Phân tích sàng lọc nghi ngờ đã được sử dụng để xác định tạm thời 32 CEC trong các mẫu, phần lớn trong số đó là dược phẩm và các sản phẩm chăm sóc cá nhân. Sử dụng SBSE tìm thấy nhiều CEC hơn so với LLE, đặc biệt là trong các mẫu nước mặt, trong đó 13 CEC được xác định tạm thời trong các mẫu SBSE so với 6 CEC trong các mẫu LLE."} +{"idx": 1870210, "en": "MicroRNAs (miRNAs) play an important role in various biological processes and have been regarded as promising diagnostic biomarkers for solid tumors in the field of clinical diagnostics. In this study, we developed a simple label/quencher-free fluorometric system for sensitive and selective miRNA detection using isothermal gene amplification such as rolling circle amplification generating tandem G-quadruplex DNA structures (GQ-RCA). The closed-circular dumbbell-shaped padlock DNA was designed to be complementary to its corresponding target miRNA. In the presence of the target miRNA, a long stretch of ssDNA with tandem G-quadruplex sequence repeats was readily generated by RCA, initiated by phi29 DNA polymerase through DNA synthesis priming at the 3'-OH of the target miRNA annealed to the padlock DNA. The RCA product harboring tandem G-quadruplex was monitored with fluorophore Thioflavin T (ThT) that emits strong fluorescence only when it intercalates into the G-quadruplex. The GQ-RCA assay enabled us to detect miRNA as low as 4.9 fM with a linear range from 25.6 fM to 80 pM within 0.5 h. In addition, our system was applied to the miRNA samples present in human plasma, showing its potential use in the clinical diagnosis of cancer.", "gemini": "MicroRNA (miRNA) đóng vai trò quan trọng trong nhiều quá trình sinh học khác nhau và được coi là các dấu ấn sinh học chẩn đoán đầy hứa hẹn cho các khối u đặc trong lĩnh vực chẩn đoán lâm sàng. Trong nghiên cứu này, chúng tôi đã phát triển một hệ thống đo huỳnh quang không có nhãn/chất dập tắt đơn giản để phát hiện miRNA một cách nhạy và chọn lọc bằng cách sử dụng phương pháp khuếch đại gen đẳng nhiệt như khuếch đại vòng tròn tạo ra các cấu trúc DNA G-tứ liên tiếp (GQ-RCA). DNA khóa hình quả tạ tròn khép kín được thiết kế để bổ sung cho miRNA đích tương ứng của nó. Khi có mặt miRNA đích, một đoạn ssDNA dài với các trình tự G-tứ liên tiếp lập lại được tạo ra dễ dàng bởi RCA, được khởi đầu bởi DNA polymerase phi29 thông qua mồi tổng hợp DNA tại 3'-OH của miRNA đích được ủ với DNA khóa. Sản phẩm RCA chứa G-tứ liên tiếp được theo dõi bằng chất huỳnh quang Thioflavin T (ThT) chỉ phát ra huỳnh quang mạnh khi nó xen kẽ vào G-tứ liên tiếp. Xét nghiệm GQ-RCA cho phép chúng tôi phát hiện miRNA thấp tới 4,9 fM với phạm vi tuyến tính từ 25,6 fM đến 80 pM trong vòng 0,5 giờ. Ngoài ra, hệ thống của chúng tôi đã được áp dụng cho các mẫu miRNA có trong huyết tương người, cho thấy tiềm năng sử dụng của nó trong chẩn đoán ung thư lâm sàng."} +{"idx": 1870211, "en": "The unsaturated metalloid germanium cluster Ge6{N(SiMe3)Dipp}42 with two ligand-free germanium atoms and only four amine substituents was obtained starting from the base-coordinated germylene {N(SiMe3)Dipp}GeCl·DMAP 1 in 50% yield (DMAP = 4-(dimethylamino)-pyridine). This cluster reacts as a masked digermyne in cycloadditions with ethylene, diphenylacetylene and 2,3-dimethyl-1,3-butadiene in toluene at 100 °C to yield 3-5.", "gemini": "Chùm germanium phi kim loại chưa bão hòa Ge6{N(SiMe3)Dipp}42 với hai nguyên tử germanium không có phối tử và chỉ bốn chất thế amin thu được bắt đầu từ germylene được phối hợp bazơ {N(SiMe3)Dipp}GeCl·DMAP 1 với năng suất 50% (DMAP = 4-(dimethylamino)-pyridine). Chùm này phản ứng như một digermyne có mặt nạ trong phản ứng cộng vòng với ethylen, diphenylacetylen và 2,3-dimethyl-1,3-butadien trong toluen ở 100 °C để tạo ra 3-5."} +{"idx": 1870212, "en": "Semiconductor-liquid interfaces are essential to the operation of many energy devices. Crucially, the operational characteristics of such devices are dependent upon both the flat band potential and doping concentration present in their solid-state semiconducting region. Traditionally, capacitive \"linear\" Mott-Schottky plots have often been utilized to extract these two parameters. However, significant concentrations of surface states within semiconductor-liquid junctions can give rise to strong non-linearities that prevent an effective linearity-based analysis. In this work, we detail a theoretical approach for estimating both the doping concentration and flat band potential from the capacitive characteristics of semiconductor-liquid junctions heavily impacted upon by surface states. Our theoretical approach is applied to CuGaS2 immersed in an aqueous electrolyte, for which excellent convergent values of the doping concentration and flat band potential are obtained across a wide range of impedance measurement frequencies. The results suggest a marked improvement over a linearity-based approach that could assist the analysis of many types of semiconductor-liquid junctions subject to high concentrations of surface states.", "gemini": "Giao diện chất bán dẫn-lỏng rất cần thiết cho hoạt động của nhiều thiết bị năng lượng. Một cách quan trọng, các đặc tính hoạt động của các thiết bị như vậy phụ thuộc vào cả điện thế dải phẳng và nồng độ tạp chất có trong vùng bán dẫn trạng thái rắn của chúng. Theo truyền thống, các đồ thị Mott-Schottky \"tuyến tính\" điện dung thường được sử dụng để trích xuất hai thông số này. Tuy nhiên, nồng độ đáng kể các trạng thái bề mặt trong các tiếp giáp chất bán dẫn-lỏng có thể gây ra các phi tuyến tính mạnh ngăn cản phân tích dựa trên tính tuyến tính hiệu quả. Trong công trình này, chúng tôi trình bày chi tiết một phương pháp tiếp cận lý thuyết để ước tính cả nồng độ tạp chất và điện thế dải phẳng từ các đặc tính điện dung của các tiếp giáp chất bán dẫn-lỏng bị ảnh hưởng nặng nề bởi các trạng thái bề mặt. Phương pháp tiếp cận lý thuyết của chúng tôi được áp dụng cho CuGaS2 ngâm trong chất điện phân nước, trong đó các giá trị hội tụ tuyệt vời của nồng độ tạp chất và điện thế dải phẳng thu được trên một phạm vi rộng các tần số đo trở kháng. Các kết quả cho thấy sự cải thiện đáng kể so với phương pháp tiếp cận dựa trên tính tuyến tính có thể hỗ trợ phân tích nhiều loại tiếp giáp chất bán dẫn-lỏng chịu nồng độ trạng thái bề mặt cao."} +{"idx": 1870213, "en": "Miniaturization of electrochemical detection methods for point-of-care-devices is ideal for their integration and use within healthcare environments. Simultaneously, the prolific pathogenic bacteria Pseudomonas aeruginosa poses a serious health risk to patients with compromised immune systems. Recognizing these two factors, a proof-of-concept electrochemical method employing a micro-interface between water and oil (w/o) held at the tip of a pulled borosilicate glass capillary is presented. This method targets small molecules produced by P. aeruginosa colonies as signalling factors that control colony growth in a pseudo-multicellular process known as quorum sensing (QS). The QS molecules of interest are 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3,4-dihydroxyquinoline (PQS, Pseudomonas quinolone signal). Hydrophobic HHQ and PQS molecules, dissolved in the oil phase, were observed electrochemically to facilitate proton transfer across the w/o interface. This interfacial complexation can be exploited as a facile electrochemical detection method for P. aeruginosa and is advantageous as it does not depend on the redox activity of HHQ/PQS. Interestingly, the limit-of-linearity is reached as [H+] ≈ [ligand]. Density functional theory calculations were performed to determine the proton affinities and gas-phase basicities of HHQ/PQS, as well as elucidate the likely site of stepwise protonation within each molecule.", "gemini": "Việc thu nhỏ các phương pháp phát hiện điện hóa cho các thiết bị tại điểm chăm sóc là lý tưởng để tích hợp và sử dụng chúng trong môi trường chăm sóc sức khỏe. Đồng thời, vi khuẩn gây bệnh Pseudomonas aeruginosa gây ra rủi ro nghiêm trọng cho sức khỏe đối với những bệnh nhân có hệ thống miễn dịch suy yếu. Nhận ra hai yếu tố này, một phương pháp điện hóa chứng minh khái niệm sử dụng giao diện vi mô giữa nước và dầu (w/o) được giữ ở đầu mao quản thủy tinh borosilicat kéo được trình bày. Phương pháp này nhắm vào các phân tử nhỏ do các khuẩn lạc P. aeruginosa tạo ra như các yếu tố báo hiệu kiểm soát sự phát triển của khuẩn lạc trong một quá trình giả đa bào được gọi là cảm ứng số lượng (QS). Các phân tử QS được quan tâm là 4-hydroxy-2-heptylquinoline (HHQ) và 2-heptyl-3,4-dihydroxyquinoline (PQS, tín hiệu quinolone của Pseudomonas). Các phân tử HHQ và PQS kỵ nước, hòa tan trong pha dầu, được quan sát thấy bằng điện hóa để tạo thuận lợi cho quá trình truyền proton qua giao diện w/o. Sự phức tạp giao diện này có thể được khai thác như một phương pháp phát hiện điện hóa dễ dàng cho P. aeruginosa và có lợi vì nó không phụ thuộc vào hoạt động oxy hóa khử của HHQ/PQS. Điều thú vị là giới hạn tuyến tính đạt được khi [H+] ≈ [ligand]. Các tính toán lý thuyết hàm mật độ đã được thực hiện để xác định ái lực proton và tính bazơ trong pha khí của HHQ/PQS, cũng như làm sáng tỏ vị trí có khả năng xảy ra quá trình proton hóa từng bước trong mỗi phân tử."} +{"idx": 1870214, "en": "Novel bioinorganic hybrid materials based on proteins and inorganic clusters have enormous potential for the development of hybrid catalysts that synergistically combine properties of both materials. Here we report the creation of hybrid assemblies between a computationally designed symmetrical protein Pizza6-S and different polyoxometalates with matching symmetry: the tellurotungstic Anderson-Evans (Na6[TeW6O24]·22H2O) (TEW); Keggin (H4[SiW12O40]·6H2O) (STA); and 1 : 2 CeIII-substituted Keggin (K11[CeIII[PW11O39]2]·20H2O) (Ce-K). This results in the formation of complexes with clearly defined stoichiometries in solution. Crystal structures validate the complexes as building blocks for the formation of larger assemblies.", "gemini": "Các vật liệu lai vô cơ sinh học mới dựa trên protein và các cụm vô cơ có tiềm năng rất lớn để phát triển các chất xúc tác lai kết hợp hiệp đồng các tính chất của cả hai vật liệu. Tại đây, chúng tôi báo cáo việc tạo ra các hợp chất lai giữa một loại protein đối xứng được thiết kế bằng máy tính Pizza6-S và các polyoxometalat khác nhau có tính đối xứng phù hợp: tellurotungstic Anderson-Evans (Na6[TeW6O24]·22H2O) (TEW); Keggin (H4[SiW12O40]·6H2O) (STA); và Keggin thế CeIII tỷ lệ 1:2 (K11[CeIII[PW11O39]2]·20H2O) (Ce-K). Điều này dẫn đến sự hình thành các phức hợp có tỷ lượng thành phần được xác định rõ ràng trong dung dịch. Cấu trúc tinh thể xác nhận các phức hợp là khối xây dựng để hình thành các hợp chất lớn hơn."} +{"idx": 1870215, "en": "Strong covalent bonds were in situ constructed in a unique black phosphorus (BP) @ reduced graphene oxide nanocomposite via a facile and scalable high energy ball-milling process, which contributes to the high specific capacity of BP@irGO and tackles the long-standing problem of large volume expansion of high capacity electrode matrerials.", "gemini": "Các liên kết cộng hóa trị mạnh được tạo ra tại chỗ trong một vật liệu nano tổng hợp phốt pho đen (BP) @ oxit graphene đã khử độc đáo thông qua một quá trình nghiền bi năng lượng cao dễ dàng và có thể mở rộng, góp phần vào dung lượng riêng cao của BP@irGO và giải quyết vấn đề lâu dài về sự giãn nở thể tích lớn của vật liệu điện cực có dung lượng cao."} +{"idx": 1870216, "en": "Diabetes mellitus (DM) is characterized by the irreversible destruction of insulin-secreting pancreatic β-islet cells and requires life-long exogenous insulin therapy. Umbilical cord Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) have been shown to improve islet function in animal models of diabetes. However, inadequate MSC homing to injured sites has limited their efficacy. Since efficient cell therapy heavily relies on appropriate homing to target tissues, increasing the specificity to the target organ and the extent of homing of the injected WJ-MSCs is paramount to successful clinical outcomes. Therefore, in this study, we synthesized Fe3O4@polydopamine nanoparticle (NP)-labeled MSCs and evaluated their therapeutic efficacy in a clinically relevant rat model of streptozotocin-induced diabetes using an external magnetic field. We found that NPs were successfully incorporated into WJ-MSCs and did not negatively affect stem cell properties. Magnetic targeting of WJ-MSCs contributed to long-term cell retention in pancreatic tissue and improved the islet function of diabetic rats, compared to injection of WJ-MSC alone. In addition, anti-inflammatory effects and the anti-apoptotic capacity of WJ-MSCs appeared to play a major role in the functional and structural recovery of the pancreas. Thus, therapy relying on the magnetic targeting of WJ-MSCs may serve as an effective approach for DM treatment.", "gemini": "Đái tháo đường (ĐTĐ) được đặc trưng bởi sự phá hủy không hồi phục các tế bào β đảo tụy tiết insulin và đòi hỏi phải điều trị insulin ngoại sinh suốt đời. Các tế bào gốc trung mô có nguồn gốc từ Wharton của dây rốn (WJ-MSC) đã được chứng minh là cải thiện chức năng đảo tụy ở các mô hình động vật mắc bệnh tiểu đường. Tuy nhiên, việc MSC không đi về đúng vị trí bị thương đã hạn chế hiệu quả của chúng. Vì liệu pháp tế bào hiệu quả phụ thuộc rất nhiều vào việc đi về đúng vị trí của các mô đích, nên việc tăng tính đặc hiệu đối với cơ quan đích và mức độ đi về đúng vị trí của các WJ-MSC được tiêm là tối quan trọng để đạt được kết quả lâm sàng thành công. Do đó, trong nghiên cứu này, chúng tôi đã tổng hợp các MSC được gắn nhãn hạt nano Fe3O4@polydopamine (NP) và đánh giá hiệu quả điều trị của chúng trên mô hình chuột liên quan đến lâm sàng bị tiểu đường do streptozotocin gây ra bằng cách sử dụng từ trường ngoài. Chúng tôi thấy rằng các NP đã được đưa thành công vào WJ-MSC và không ảnh hưởng tiêu cực đến các đặc tính của tế bào gốc. Việc nhắm mục tiêu từ tính vào WJ-MSC góp phần duy trì tế bào lâu dài trong mô tụy và cải thiện chức năng đảo tụy của chuột mắc bệnh tiểu đường, so với việc chỉ tiêm WJ-MSC. Ngoài ra, tác dụng chống viêm và khả năng chống apoptosis của WJ-MSC dường như đóng vai trò chính trong sự phục hồi chức năng và cấu trúc của tuyến tụy. Do đó, liệu pháp dựa trên việc nhắm mục tiêu từ tính vào WJ-MSC có thể đóng vai trò là một phương pháp tiếp cận hiệu quả để điều trị ĐTĐ."} +{"idx": 1870217, "en": "Enantioselective synthesis of tetrahydroisoquinolines bearing an all-carbon quaternary stereogenic center, was achieved via asymmetric C-H activation with high enantioselectivities (up to 93% ee). Fair substrate tolerance was indicated throughout the scope investigation and no evident loss of enantioselectivity was exhibited in late-stage derivatization. This study provides incentives for the construction of diverse chiral isoquinoline derivatives, which are prevalent among pharmaceuticals, natural products, etc.", "gemini": "Tổng hợp bất đối quang của tetrahydroisoquinoline mang một trung tâm lập thể bậc bốn toàn cacbon đã đạt được thông qua hoạt hóa C-H không đối xứng với độ bất đối quang cao (lên đến 93% ee). Dung sai chất nền khá được chỉ ra trong suốt quá trình điều tra phạm vi và không có sự mất độ bất đối quang nào được thể hiện trong quá trình dẫn xuất giai đoạn muộn. Nghiên cứu này cung cấp động lực để xây dựng các dẫn xuất isoquinoline đa dạng có tính quang học, phổ biến trong số các dược phẩm, sản phẩm tự nhiên, v.v."} +{"idx": 1870218, "en": "An interface between our in-house DynaVib package and quantum chemistry software Gamess-US is implemented for computing vibrationally-resolved K-edge X-ray photoelectron spectra (XPS) of molecules at the density functional theory level with both the full (FCH) and equivalent (ECH, or Z+1) core-hole approximations. To assess the influence of theoretical parameters (core-hole methods, vibronic coupling models, and basis sets), vibrationally-resolved C1s XPS of six simple cyclic molecules [furan, pyrrole, thiophene; benzene (C6H6 and C6D6); pyridine] were evaluated in the gas phase by both core-hole methods in combination with two time-independent vibronic coupling models, the Duschinsky rotation (DR) method and the linear coupling model (LCM). We achieved excellent/acceptable performance for FCH/Z+1 simulations in comparison with experiments. The most accurate method FCH-DR correctly reproduced all experimental features and gave an accuracy of ca. 0.2 eV in absolute binding energies (BEs). The choice of the vibronic model is less sensitive to that of electronic structure method. Results indicate that Z+1 overestimates the core-hole effect on the geometry of the ionization state.", "gemini": "Một giao diện giữa gói DynaVib nội bộ của chúng tôi và phần mềm hóa học lượng tử Gamess-US được triển khai để tính toán phổ quang điện tử tia X (XPS) vùng K được giải quyết theo độ rung của các phân tử ở mức lý thuyết hàm mật độ chức năng với cả hai phương pháp xấp xỉ lỗ lõi đầy đủ (FCH) và tương đương (ECH hoặc Z+1). Để đánh giá ảnh hưởng của các thông số lý thuyết (phương pháp lỗ lõi, mô hình ghép rung và bộ cơ sở), XPS C1s được giải quyết theo độ rung của sáu phân tử vòng đơn giản [furan, pyrrole, thiophene; benzen (C6H6 và C6D6); pyridine] đã được đánh giá trong pha khí bằng cả hai phương pháp lỗ lõi kết hợp với hai mô hình ghép rung độc lập thời gian, phương pháp quay Duschinsky (DR) và mô hình ghép tuyến tính (LCM). Chúng tôi đạt được hiệu suất tuyệt vời/chấp nhận được cho các mô phỏng FCH/Z+1 khi so sánh với các thí nghiệm. Phương pháp chính xác nhất FCH-DR đã tái tạo chính xác tất cả các đặc điểm thực nghiệm và cho độ chính xác khoảng 0,2 eV về năng lượng liên kết tuyệt đối (BE). Sự lựa chọn mô hình rung ít nhạy cảm hơn so với phương pháp cấu trúc điện tử. Kết quả chỉ ra rằng Z+1 đánh giá quá cao hiệu ứng lỗ lõi đối với hình học của trạng thái ion hóa."} +{"idx": 1870219, "en": "On-line attenuated total reflection infrared spectroscopy (ATR-IR) was used to gain a good understanding of the kinetics and mechanism for methyl cyclopentenone (MCP) synthesis from 2-methylfuran and formaldehyde in a four-step reaction. Combining in situ IR monitoring and a quantitative univariate model, the mechanisms for the main side reactions were discussed in depth. The presence and forming mechanism of the side product generated in step 1 (Mannich reaction) were reported for the first time. Off-line 1H NMR and GC-MS were used as reference tools to further clarify the structure of the side product. Results also show that an undesirable side reaction will take place if the reaction time for step 2 is longer than 3 h. Possible mechanisms for side reactions and optimized experimental conditions were suggested for the purpose of improving the selectivity of the main reaction to efficiently facilitate the yield of MCP. The present study demonstrates that on-line ATR-IR can be a powerful tool to gain insight into the process understanding of various chemical reactions, providing a solid theoretical foundation for highly efficient, large-scale synthesis of MCP.", "gemini": "Sử dụng phổ hồng ngoại phản xạ toàn phần suy yếu trực tuyến (ATR-IR) để hiểu rõ về động học và cơ chế tổng hợp methyl cyclopentenon (MCP) từ 2-methylfuran và formaldehyd trong phản ứng bốn bước. Kết hợp theo dõi IR tại chỗ và mô hình đơn biến định lượng, các cơ chế cho các phản ứng phụ chính đã được thảo luận sâu. Sự hiện diện và cơ chế hình thành sản phẩm phụ tạo ra ở bước 1 (phản ứng Mannich) đã được báo cáo lần đầu tiên. 1H NMR ngoại tuyến và GC-MS được sử dụng làm công cụ tham chiếu để làm rõ thêm cấu trúc của sản phẩm phụ. Kết quả cũng cho thấy rằng sẽ xảy ra phản ứng phụ không mong muốn nếu thời gian phản ứng cho bước 2 dài hơn 3 giờ. Các cơ chế có thể xảy ra đối với các phản ứng phụ và điều kiện thực nghiệm tối ưu đã được đề xuất với mục đích cải thiện tính chọn lọc của phản ứng chính để tạo điều kiện hiệu quả cho năng suất của MCP. Nghiên cứu hiện tại chứng minh rằng ATR-IR trực tuyến có thể là một công cụ mạnh mẽ để hiểu sâu hơn về quá trình hiểu biết về các phản ứng hóa học khác nhau, cung cấp nền tảng lý thuyết vững chắc cho quá trình tổng hợp MCP quy mô lớn, hiệu suất cao."} +{"idx": 1870220, "en": "The photo-isomerization of an isolable five-membered singlet biradical based on C, N, and P ([TerNP]2CNDmp, 2a) selectively afforded a closed-shell housane-type isomer (3a) by forming a transannular P-P bond. In the dark, the housane-type species re-isomerized to the biradical, resulting in a fully reversible overall process. In the present study, the influence of tBuNC on the thermal reverse reaction was investigated: the isonitrile acted as a catalyst, thus allowing control over the thermal reaction rate. Moreover, tBuNC also reacted with the biradical to form an adduct species ([TerNP]2CNDmp·CNtBu, 4a), which can be regarded as the resting state of the system. The reactive species 2a and 3a could be re-generated in situ by irradiation with red light. The results of this study extend our understanding of this new class of molecular switches.", "gemini": "Sự đồng phân hóa ảnh của một biradical đơn năm thành viên có thể cô lập dựa trên C, N và P ([TerNP]2CNDmp, 2a) có chọn lọc tạo ra một đồng phân loại housane vỏ kín (3a) bằng cách hình thành liên kết P-P xuyên vòng. Trong bóng tối, các loài loại housane đồng phân lại thành biradical, dẫn đến quá trình tổng thể có thể đảo ngược hoàn toàn. Trong nghiên cứu hiện tại, ảnh hưởng của tBuNC đối với phản ứng đảo ngược nhiệt đã được nghiên cứu: isonitrile đóng vai trò như một chất xúc tác, do đó cho phép kiểm soát tốc độ phản ứng nhiệt. Hơn nữa, tBuNC cũng phản ứng với biradical để tạo thành một loài adduct ([TerNP]2CNDmp·CNtBu, 4a), có thể được coi là trạng thái nghỉ của hệ thống. Các loài phản ứng 2a và 3a có thể được tái tạo tại chỗ bằng cách chiếu sáng đỏ. Kết quả của nghiên cứu này mở rộng sự hiểu biết của chúng ta về lớp công tắc phân tử mới này."} +{"idx": 1870221, "en": "The ability to directly recognize double-stranded DNA (ds-DNA) is a major challenge in disease diagnosis and gene therapy because DNA is naturally double-stranded. Herein, a novel electrochemical biosensor for the sequence-specific recognition of ds-DNA using a peptide nucleic acid (PNA) probe and graphene oxide (GO) modified pencil graphite electrode is reported and applied for the direct detection of the desired sequence in plasmid samples. For this purpose, GO was assembled onto the pencil graphite electrode surface (GO/PGE) by a simple casting method and applied for PNA probe immobilization (PNA-GO/PGE). Upon addition of ds-DNA, the interaction of the PNA probe with ds-DNA induces probe detachment from the electrode surface which results in a guanine oxidation signal decrease. Under optimized conditions, the guanine oxidation signal decreased linearly with the ds-DNA concentration increasing in the range from 30 pM to 10 nM, with a detection limit of 1.3 pM. Moreover, the proposed biosensor was applied for the sensitive and selective detection of double-stranded target DNA in plasmid samples. This proposed method could be used as a platform for direct detection of various sequences in double-stranded genomic DNA.", "gemini": "Khả năng nhận biết trực tiếp DNA mạch kép (ds-DNA) là một thách thức lớn trong chẩn đoán bệnh và liệu pháp gen vì DNA tự nhiên là mạch kép. Trong bài báo này, một cảm biến sinh học điện hóa mới để nhận biết trình tự đặc hiệu của ds-DNA bằng cách sử dụng đầu dò axit nucleic peptide (PNA) và điện cực than chì bút chì được sửa đổi bằng graphene oxit (GO) được báo cáo và áp dụng để phát hiện trực tiếp trình tự mong muốn trong mẫu plasmid. Với mục đích này, GO đã được lắp ráp lên bề mặt điện cực than chì bút chì (GO/PGE) bằng phương pháp đúc đơn giản và được áp dụng để cố định đầu dò PNA (PNA-GO/PGE). Khi bổ sung ds-DNA, sự tương tác của đầu dò PNA với ds-DNA sẽ gây ra sự tách đầu dò khỏi bề mặt điện cực, dẫn đến tín hiệu oxy hóa guanine giảm. Trong điều kiện tối ưu, tín hiệu oxy hóa guanine giảm tuyến tính khi nồng độ ds-DNA tăng trong khoảng từ 30 pM đến 10 nM, với giới hạn phát hiện là 1,3 pM. Hơn nữa, cảm biến sinh học được đề xuất đã được áp dụng để phát hiện nhạy và chọn lọc DNA đích mạch kép trong các mẫu plasmid. Phương pháp được đề xuất này có thể được sử dụng như một nền tảng để phát hiện trực tiếp các trình tự khác nhau trong DNA bộ gen mạch kép."} +{"idx": 1870222, "en": "As an important and universal tumor marker, the reliable and in situ detection of intracellular telomerase activity is crucial for cancer diagnosis. Herein, a ratiometric fluorescence resonance energy transfer (FRET) method was developed for detecting intracellular telomerase activity. It takes full advantage of manganese dioxide nanosheets (MnO2NS) that can carry DNA probes with different conformations into cells and then completely release the DNA probes via decomposition of MnO2NS by intracellular reduced glutathione (GSH). In the presence of telomerase, a telomere substrate (TS) could be extended to form long telomerase extension products (TEPs), which trigger the cycling strand displacement reaction (SDR) between two fluorophore-labeled hairpin DNA probes to form lots of DNA duplexes. The close contact of two fluorophores led to an effective ratiometric FRET for reliable detection of telomerase activity. Fluorescence confocal imaging demonstrated that the activity of telomerase in tumor cells was reliably detected. The inhibition of telomerase activity by an inhibitor resulted in a decrease in FRET signal. For extracellular detection, the FRET ratio (FA/FD) shows a good linear relationship with the number of HeLa cells in the range of 20-1000 cells. Therefore, it offers a more facile method for reliable and sensitive detection of intracellular telomerase activity.", "gemini": "Là một dấu ấn khối u quan trọng và phổ quát, việc phát hiện đáng tin cậy và tại chỗ hoạt động của telomerase nội bào rất quan trọng đối với chẩn đoán ung thư. Ở đây, một phương pháp truyền năng lượng cộng hưởng huỳnh quang tỷ lệ (FRET) đã được phát triển để phát hiện hoạt động của telomerase nội bào. Nó tận dụng tối đa các tấm nano dioxide mangan (MnO2NS) có thể mang các đầu dò DNA với các cấu hình khác nhau vào tế bào và sau đó giải phóng hoàn toàn các đầu dò DNA thông qua quá trình phân hủy MnO2NS bởi glutathione khử nội bào (GSH). Với sự hiện diện của telomerase, một chất nền telomere (TS) có thể được kéo dài để tạo thành các sản phẩm kéo dài telomerase dài (TEP), kích hoạt phản ứng dịch chuyển chuỗi tuần hoàn (SDR) giữa hai đầu dò DNA kẹp tóc được gắn huỳnh quang để tạo thành nhiều phức hợp DNA. Tiếp xúc gần của hai huỳnh quang dẫn đến FRET tỷ lệ hiệu quả để phát hiện đáng tin cậy hoạt động của telomerase. Chụp ảnh cộng hưởng huỳnh quang đã chứng minh rằng hoạt động của telomerase trong các tế bào khối u được phát hiện một cách đáng tin cậy. Sự ức chế hoạt động của telomerase bởi chất ức chế dẫn đến giảm tín hiệu FRET. Đối với phát hiện ngoại bào, tỷ lệ FRET (FA/FD) cho thấy mối quan hệ tuyến tính tốt với số lượng tế bào HeLa trong khoảng 20-1000 tế bào. Do đó, nó cung cấp một phương pháp dễ dàng hơn để phát hiện đáng tin cậy và nhạy hoạt động của telomerase nội bào."} +{"idx": 1870223, "en": "An unprecedented triple catalytic, general ring-opening cyanation reaction of cyclopropyl ketones for the construction of γ-cyanoketones is described. The key is to merge photoredox catalysis with Lewis acid catalysis and copper catalysis to enable the selective cleavage of the carbon-carbon bonds and the selective coupling of the generated radical and cyanide anion.", "gemini": "Một phản ứng xyanua mở vòng chung, xúc tác ba chất xúc tác chưa từng có của xeton cyclopropyl để xây dựng γ-cyanoketon được mô tả. Chìa khóa là hợp nhất xúc tác quang với xúc tác axit Lewis và xúc tác đồng để cho phép cắt chọn lọc các liên kết carbon-carbon và liên kết chọn lọc của gốc được tạo ra và anion xyanua."} +{"idx": 1870224, "en": "Defect engineering is increasingly recognized as a viable strategy for boosting the performance of photoelectrochemical (PEC) water splitting using metal oxide-based photoelectrodes. However, previously developed methods for generating point defects associated with oxygen vacancies are rather time-consuming. Herein, high density oxygen deficient α-Fe2O3 with the dominant (110) crystal plane is developed in a very short timescale of 10 minutes by employing aerosol-assisted chemical vapor deposition and pure nitrogen as a gas carrier. The oxygen-defective film exhibits almost 8 times higher photocurrent density compared to a hematite photoanode with a low concentration of oxygen vacancies which is prepared in purified air. The existence of oxygen vacancies improves light absorption ability, accelerates charge transport in the bulk of films, and promotes charge separation at the electrolyte/semiconductor interface. DFT simulations verify that oxygen-defective hematite has a narrow bandgap, electron-hole trapped centre, and strong adsorption energy of water molecules compared to pristine hematite. This strategy might bring PEC technology another step further towards large-scale fabrication for future commercialization.", "gemini": "Kỹ thuật khuyết tật ngày càng được công nhận là một chiến lược khả thi để tăng cường hiệu suất của quá trình tách nước quang điện hóa (PEC) sử dụng các điện cực quang điện tử dựa trên oxit kim loại. Tuy nhiên, các phương pháp được phát triển trước đây để tạo ra các khuyết tật điểm liên quan đến khuyết hổng oxy khá tốn thời gian. Ở đây, α-Fe2O3 thiếu oxy mật độ cao với mặt phẳng tinh thể (110) chiếm ưu thế được phát triển trong khoảng thời gian rất ngắn 10 phút bằng cách sử dụng lắng đọng hơi hóa học hỗ trợ bằng khí dung và nitơ tinh khiết làm chất mang khí. Màng khuyết oxy thể hiện mật độ dòng quang cao hơn gần 8 lần so với anot quang hematit có nồng độ khuyết hổng oxy thấp được chế tạo trong không khí tinh khiết. Sự tồn tại của các khuyết hổng oxy cải thiện khả năng hấp thụ ánh sáng, tăng tốc quá trình vận chuyển điện tích trong khối màng và thúc đẩy quá trình tách điện tích tại giao diện chất điện phân/bán dẫn. Các mô phỏng DFT xác minh rằng hematit khuyết oxy có khoảng cách dải hẹp, tâm bẫy lỗ điện tử và năng lượng hấp phụ mạnh các phân tử nước so với hematit nguyên chất. Chiến lược này có thể đưa công nghệ PEC tiến thêm một bước nữa hướng tới sản xuất quy mô lớn để thương mại hóa trong tương lai."} +{"idx": 1870225, "en": "Bisphenol A (BPA) is used as a stabilizing agent in many food packaging plastics and is a known endocrine-disrupting chemical that can alter the development of mammary glands, affect egg cells, and cause chromosomal defects. However, the pretreatment of traditional assays for detecting BPA is difficult. In this work, a novel aptamer functionalized magnetic adsorbent was developed and combined with magnetic solid-phase extraction (MSPE) for the selective enrichment of BPA. First, magnetic silica-coated Fe3O4 microspheres (Fe3O4@SiO2) were synthesized by the sol-gel method, and functional magnetic nanoparticles (Fe3O4@SiO2@Apt) were formed by modifying with nucleic acids. In the presence of BPA in a MSPE system, the nucleic acid aptamer can specifically capture the target BPA. After magnetic separation, the Apt/BPA composite was eluted, and we observed enhanced fluorescence with the Apt/BPA composite that was formed. Our results showed that this method allowed a limit of detection of 0.05 ng mL-1.", "gemini": "Bisphenol A (BPA) được sử dụng như một tác nhân ổn định trong nhiều loại nhựa đóng gói thực phẩm và là một hóa chất gây rối loạn nội tiết đã biết có thể làm thay đổi sự phát triển của tuyến vú, ảnh hưởng đến tế bào trứng và gây ra dị tật nhiễm sắc thể. Tuy nhiên, việc xử lý trước các xét nghiệm truyền thống để phát hiện BPA rất khó khăn. Trong nghiên cứu này, một chất hấp phụ từ tính chức năng hóa aptamer mới đã được phát triển và kết hợp với chiết xuất pha rắn từ tính (MSPE) để làm giàu BPA có chọn lọc. Đầu tiên, các vi cầu Fe3O4 phủ silica từ tính (Fe3O4@SiO2) được tổng hợp bằng phương pháp sol-gel, và các hạt nano từ tính chức năng (Fe3O4@SiO2@Apt) được hình thành bằng cách biến đổi với axit nucleic. Với sự hiện diện của BPA trong hệ thống MSPE, aptamer axit nucleic có thể bắt giữ đặc hiệu BPA mục tiêu. Sau khi tách từ tính, hợp chất Apt/BPA được rửa giải và chúng tôi quan sát thấy huỳnh quang tăng cường với hợp chất Apt/BPA được hình thành. Kết quả của chúng tôi cho thấy phương pháp này cho phép giới hạn phát hiện là 0,05 ng mL-1."} +{"idx": 1870226, "en": "Thermodynamic models of solid solutions used in computational thermochemistry have not been modernized in recent years. With the advent of fast and cheap computers, it is nowadays possible to add, at a minimal computational cost, physical ingredients such as coordination numbers, inter-atomic distances and classical interatomic potentials to the function describing the energetics of ordered and disordered solid solutions. As we show here, the integration of these elements into a robust statistical thermodynamic model of solution establishes natural connections with other deterministic and stochastic atomistic methods such as Monte Carlo and molecular dynamics simulations. Ultimately, all these numerical approaches need to be self-consistent and generate complementary sets of numerical thermo-physical properties. The present work proposes a new formalism to define the Gibbs free energy of ordered and disordered solid solutions. It allows for a complete prediction of the thermal, volumetric and compositional dependence of the Gibbs free energy by solving a constrained minimization problem. As a proof of concept, we explore the energetic behavior of pure face-centered cubic gold as well as the AuCu L10 ordered solution as a function of both temperature and pressure. We finally compare these results with the average properties obtained from classical molecular dynamics simulations and explain the origin of the existing differences between the two approaches based on how the temperature is accounted for in each method.", "gemini": "Các mô hình nhiệt động lực học của các dung dịch rắn được sử dụng trong nhiệt hóa học tính toán đã không được hiện đại hóa trong những năm gần đây. Với sự ra đời của máy tính nhanh và giá rẻ, hiện nay có thể thêm các thành phần vật lý như số phối hợp, khoảng cách giữa các nguyên tử và thế liên nguyên tử cổ điển vào hàm mô tả năng lượng của các dung dịch rắn có trật tự và không trật tự với chi phí tính toán tối thiểu. Như chúng tôi trình bày ở đây, việc tích hợp các yếu tố này vào một mô hình nhiệt động lực học thống kê mạnh mẽ của dung dịch thiết lập các kết nối tự nhiên với các phương pháp nguyên tử xác định và ngẫu nhiên khác như mô phỏng Monte Carlo và động lực học phân tử. Cuối cùng, tất cả các cách tiếp cận số này cần phải tự nhất quán và tạo ra các tập hợp bổ sung các tính chất nhiệt - vật lý số. Công trình hiện tại đề xuất một hình thức mới để xác định năng lượng tự do Gibbs của các dung dịch rắn có trật tự và không trật tự. Nó cho phép dự đoán hoàn chỉnh sự phụ thuộc nhiệt, thể tích và thành phần của năng lượng tự do Gibbs bằng cách giải một bài toán tối thiểu bị ràng buộc. Như một minh chứng cho khái niệm, chúng tôi khám phá hành vi năng lượng của vàng lập phương tâm mặt tinh khiết cũng như dung dịch có trật tự AuCu L10 như một hàm của cả nhiệt độ và áp suất. Cuối cùng, chúng tôi so sánh các kết quả này với các tính chất trung bình thu được từ các mô phỏng động lực học phân tử cổ điển và giải thích nguồn gốc của sự khác biệt hiện có giữa hai phương pháp dựa trên cách nhiệt độ được tính đến trong mỗi phương pháp."} +{"idx": 1870227, "en": "Type II intramolecular cycloadditions ([4+2], [4+3], [4+4] and [5+2]) have emerged recently as an efficient and powerful strategy for the construction of bridged ring systems. In general, type II cycloadditions provide access to a wide range of bridged bicyclo[m.n.1] ring systems with high regio- and diastereoselectivity in an easy and straightforward manner. In each section of this review, an overview of the corresponding type II cycloadditions is presented, which is followed by highlights of method development and synthetic applications in natural product synthesis. The goal of this review is to provide a survey of recent advances in the field covering literature up to 2020. The review will serve as a useful reference for organic chemists engaged in the total synthesis of natural products containing bridged bicyclo[m.n.1] ring systems and provide strong stimulus for invention and further advances in this exciting research field.", "gemini": "Các phản ứng cycloaddition phân tử trong loại II ([4+2], [4+3], [4+4] và [5+2]) gần đây đã xuất hiện như một chiến lược hiệu quả và mạnh mẽ để xây dựng các hệ thống vòng cầu nối. Nhìn chung, các phản ứng cycloaddition loại II cung cấp khả năng tiếp cận nhiều hệ thống vòng bicyclo[m.n.1] cầu nối với tính chọn lọc vùng và lập thể cao theo cách dễ dàng và trực tiếp. Trong mỗi phần của bài đánh giá này, tổng quan về các phản ứng cycloaddition loại II tương ứng được trình bày, tiếp theo là các điểm nổi bật về phát triển phương pháp và ứng dụng tổng hợp trong tổng hợp sản phẩm tự nhiên. Mục tiêu của bài đánh giá này là cung cấp một cuộc khảo sát về những tiến bộ gần đây trong lĩnh vực này bao gồm các tài liệu chuyên ngành cho đến năm 2020. Bài đánh giá sẽ đóng vai trò là tài liệu tham khảo hữu ích cho các nhà hóa học hữu cơ tham gia vào quá trình tổng hợp toàn phần các sản phẩm tự nhiên có chứa các hệ thống vòng bicyclo[m.n.1] cầu nối và cung cấp động lực mạnh mẽ cho việc phát minh và tiến bộ hơn nữa trong lĩnh vực nghiên cứu thú vị này."} +{"idx": 1870228, "en": "Tremendous effort has been devoted to develop durable electrode materials for sodium ion batteries. This work focuses on enhancing the reversibility of a cathode material Na0.5Ni0.25Mn0.75O2 by adopting the titanium cation doping strategy. The obtained P2-Na0.5Ni0.25Mn0.60Ti0.15O2 material shows smooth charge-discharge curves upon suppressing the Na+/vacancy ordering effect via the partial substitution of Mn4+ for Ti4+, and enhanced cycling performance. It exhibits a reversible capacity of 138 mA h g-1 at 0.5C, as well as a high rate capacity of 81 mA h g-1 at 5C between a cut-off voltage of 2 and 4 V, while long-term cycling stability is demonstrated with a capacity retention of 84% over 200 cycles. An enhanced cycling stability is also observed when the voltage is between 2 and 4.2 V. The feasibility of constructing a symmetrical Na-ion full cell with Na0.5Ni0.25Mn0.60Ti0.15O2 as cathode and anode electrodes is also demonstrated. The titanium cation doping results in reduced charge transfer impedance and an enhanced sodium cation diffusion coefficient, thus suggesting an efficient strategy to obtain a durable cathode material for sodium ion batteries.", "gemini": "Một nỗ lực to lớn đã được dành để phát triển các vật liệu điện cực bền vững cho pin ion natri. Công trình này tập trung vào việc tăng cường khả năng đảo ngược của vật liệu catot Na0.5Ni0.25Mn0.75O2 bằng cách áp dụng chiến lược pha tạp cation titan. Vật liệu P2-Na0.5Ni0.25Mn0.60Ti0.15O2 thu được cho thấy các đường cong sạc-xả mượt mà khi ức chế hiệu ứng sắp xếp Na+/khoảng trống thông qua sự thay thế một phần Mn4+ cho Ti4+ và hiệu suất chu kỳ được tăng cường. Nó thể hiện dung lượng đảo ngược là 138 mA h g-1 ở 0,5C, cũng như dung lượng tốc độ cao là 81 mA h g-1 ở 5C giữa điện áp cắt là 2 và 4 V, trong khi độ ổn định chu kỳ dài hạn được chứng minh với khả năng duy trì dung lượng là 84% sau 200 chu kỳ. Độ ổn định chu kỳ được tăng cường cũng được quan sát thấy khi điện áp nằm trong khoảng từ 2 đến 4,2 V. Khả năng chế tạo một cell đầy đủ ion Na đối xứng với Na0.5Ni0.25Mn0.60Ti0.15O2 làm điện cực catot và anot cũng được chứng minh. Pha tạp cation titan dẫn đến giảm trở kháng truyền điện tích và hệ số khuếch tán cation natri được tăng cường, do đó gợi ý một chiến lược hiệu quả để thu được vật liệu catot bền cho pin ion natri."} +{"idx": 1870229, "en": "Referred to as the \"guardian of the genome\", p53 is the most frequently mutated protein in cancer and almost all cancers exhibit malfunction along the p53 pathway. As an overexpressed and tumour-specific target, the past two decades have seen considerable dedication to the development of small molecules that aim to restore wild-type function in mutant p53. In this review we collect and communicate the chemical principles involved in small molecule drug design for misfolded proteins in anticancer therapy. While this approach has met with significant challenges including off-target mechanisms that induce cytotoxicity independent of p53 status, major technological advancements in gene sequencing capability and a shift towards personalized medicine holds significant promise for p53 reactivating compounds and could have widespread benefits for the field of cancer therapy.", "gemini": "Được gọi là \"người bảo vệ bộ gen\", p53 là protein đột biến thường xuyên nhất trong ung thư và hầu như tất cả các loại ung thư đều biểu hiện sự trục trặc dọc theo con đường p53. Là một mục tiêu quá mức và đặc hiệu của khối u, trong hai thập kỷ qua đã chứng kiến sự cống hiến đáng kể cho việc phát triển các phân tử nhỏ nhằm mục đích khôi phục chức năng kiểu hoang dã trong p53 đột biến. Trong bài đánh giá này, chúng tôi thu thập và truyền đạt các nguyên tắc hóa học liên quan đến thiết kế thuốc phân tử nhỏ cho các protein bị gấp sai trong liệu pháp chống ung thư. Mặc dù cách tiếp cận này gặp phải những thách thức đáng kể bao gồm các cơ chế ngoài mục tiêu gây ra độc tính tế bào không phụ thuộc vào trạng thái p53, những tiến bộ công nghệ lớn trong khả năng giải trình tự gen và sự chuyển dịch sang y học cá nhân mang lại triển vọng đáng kể cho các hợp chất tái hoạt hóa p53 và có thể có lợi ích rộng rãi cho lĩnh vực liệu pháp ung thư."} +{"idx": 1870230, "en": "Functionalization of metal-organic framework (MOF) ligands can tune the adsorption properties of MOFs. The adsorptions of NO, NO2, NH3, C5H5N, C4H5N, and C4H4O on pristine and five X-functionalized HKUST-1, i.e. Cu3(BTC)2 (BTC = 1,3,5-benzenetricarboxylate) (X = CH3, CH3O, NH2, NO2, and Br) are evaluated by van der Waals corrected density functional theory calculations. Despite the fact that the open metal center is the energetically preferred adsorption site for most of them, the functional group site can yield a comparable adsorption ability with the open metal center. This is particularly true for pyrrole C4H5N adsorption on CH3O-functionalized HKUST-1 where the functional group site shows stronger adsorption stability than the open metal center site, probably due to the formed hydrogen bond between pyrrole and the CH3O functional group. While the CH3- or CH3O-functionalized organic linker in these MOFs strengthens the adsorption of all the considered species, that of NO2-, Br-, or NH2-functional groups reduces, which is associated with their topologies. Among them, only CH3- or CH3O-functionalized HKUST-1 presents the fmj (orthorhombic crystal system) topology while all the others are isostructural to the pristine HKUST-1 with the tbo (twisted boracite-type, cubic) topological structure. Among six adsorbates, two basic adsorbates, C5H5N and NH3, always yield the strongest bonding strength upon adsorption on the pristine and five functionalized HKUST-1. Electronic properties including the Bader charges, electron density differences, and electron localization function were investigated to comprehend their adsorption behaviors. This work provides guidance for the proper functionalization of HKUST-1 with improved adsorption properties for specific adsorbates.", "gemini": "Chức năng hóa các phối tử của khung kim loại-hữu cơ (MOF) có thể điều chỉnh các tính chất hấp phụ của MOF. Sự hấp phụ của NO, NO2, NH3, C5H5N, C4H5N và C4H4O trên HKUST-1 nguyên chất và năm loại chức năng hóa X, tức là Cu3(BTC)2 (BTC = 1,3,5-benzenetricarboxylate) (X = CH3, CH3O, NH2, NO2 và Br) được đánh giá bằng các phép tính lý thuyết mật độ hàm tử hiệu chỉnh van der Waals. Mặc dù thực tế là trung tâm kim loại hở là vị trí hấp phụ được ưa thích về mặt năng lượng đối với hầu hết các chất trong số chúng, vị trí nhóm chức năng có thể tạo ra khả năng hấp phụ tương đương với trung tâm kim loại hở. Điều này đặc biệt đúng đối với sự hấp phụ pyrrole C4H5N trên HKUST-1 chức năng hóa CH3O, trong đó vị trí nhóm chức năng cho thấy độ ổn định hấp phụ mạnh hơn vị trí trung tâm kim loại hở, có thể là do liên kết hydro được hình thành giữa pyrrole và nhóm chức năng CH3O. Trong khi liên kết hữu cơ chức năng hóa CH3 hoặc CH3O trong các MOF này làm tăng cường sự hấp phụ của tất cả các loài được xem xét, thì các nhóm chức năng NO2-, Br- hoặc NH2- lại làm giảm, điều này liên quan đến cấu trúc của chúng. Trong số đó, chỉ có HKUST-1 chức năng hóa CH3 hoặc CH3O thể hiện cấu trúc fmj (hệ tinh thể trực thoi) trong khi tất cả các loại khác đều có cấu trúc đồng dạng với HKUST-1 nguyên chất với cấu trúc tôpô tbo (boracite xoắn, lập phương). Trong số sáu chất hấp phụ, hai chất hấp phụ cơ bản, C5H5N và NH3, luôn tạo ra cường độ liên kết mạnh nhất khi hấp phụ trên HKUST-1 nguyên chất và năm loại chức năng hóa. Các tính chất điện tử bao gồm điện tích Bader, chênh lệch mật độ điện tử và hàm cục bộ hóa điện tử đã được nghiên cứu để hiểu hành vi hấp phụ của chúng. Công trình này cung cấp hướng dẫn để chức năng hóa HKUST-1 thích hợp với các tính chất hấp phụ được cải thiện cho các chất hấp phụ cụ thể."} +{"idx": 1870231, "en": "Ethyl and phenyl thioglycosides are the two most common types of thioglycoside donors in carbohydrate chemistry. However, the chemoselective activation of ethyl vs. phenyl thioglycosides is very rare in the literature. In this work, ethyl thioglycosides could be readily activated with an N-trifluoromethylthiosaccharin/TMSOTf system in the presence of phenyl thioglycosides carrying the same or even more armed protecting group pattern. Both armed and disarmed thioglycosides exhibited high chemoselectivity towards the promoter system. Chemoselective glycosylation was subsequently applied to one-pot synthesis, thus providing an efficient means to oligosaccharides.", "gemini": "Ethyl và phenyl thioglycosid là hai loại phổ biến nhất của các chất cho thioglycosid trong hóa học carbohydrate. Tuy nhiên, quá trình hoạt hóa hóa học chọn lọc của ethyl so với phenyl thioglycosid rất hiếm trong tài liệu. Trong công trình này, ethyl thioglycosid có thể được hoạt hóa dễ dàng với hệ thống N-trifluoromethylthiosaccharin/TMSOTf khi có sự hiện diện của phenyl thioglycosid mang cùng hoặc thậm chí có nhiều nhóm bảo vệ hơn. Cả thioglycosid có vũ trang và không có vũ trang đều thể hiện tính chọn lọc hóa học cao đối với hệ thống xúc tiến. Sau đó, quá trình glycosyl hóa chọn lọc về mặt hóa học được áp dụng cho quá trình tổng hợp một nồi, do đó cung cấp một phương tiện hiệu quả cho oligosacarit."} +{"idx": 1870232, "en": "Self-assembly of cyclodextrin (CD) with guest polymers has attracted much attention owing to its biocompatibility and accessibility. In this study, we investigate the composition effect of poly(ethylene oxide)m-b-poly(propylene oxide)n-b-poly(ethylene oxide)m (EOmPOnEOm) triblock copolymers on lamellar or plate structures formed by complexation with β-CD. EO5PO29EO5, EO14PO29EO14, and EO75PO29EO75 show periodic lamellar morphology consisting of single-crystalline pseudo-polyrotaxane (PPR) nanosheets with a thickness equal to the central PO length. This is because β-CDs selectively cover the PO component and cause the microphase separation between β-CD and EO layers. The thickness of the EO layers increases linearly with increasing number of EO units, which suggests that the EO chains are constrained into virtual cylinders with the diameter of the β-CD. This means that we can precisely control the thickness of both the crystal (β-CD and PO) and the amorphous (EO) layers in the lamellar structure. In contrast, EO2PO29EO2 forms a thin plate structure, where not only PO but also EO chains are covered with β-CD. Furthermore, the length of the central PO component is necessary to form the lamellar structure with the phase separation between the β-CD and EO layers. These findings provide a more fundamental understanding to enhance the variety and applicability of CD-based self-assembled materials.", "gemini": "Việc tự lắp ráp cyclodextrin (CD) với các polyme khách đã thu hút nhiều sự chú ý do tính tương thích sinh học và khả năng tiếp cận của nó. Trong nghiên cứu này, chúng tôi tìm hiểu hiệu ứng thành phần của các đồng trùng hợp ba khối poly(ethylene oxide)m-b-poly(propylene oxide)n-b-poly(ethylene oxide)m (EOmPOnEOm) trên các cấu trúc phiến hoặc tấm được hình thành bởi phức hợp với β-CD. EO5PO29EO5, EO14PO29EO14 và EO75PO29EO75 cho thấy hình thái phiến tuần hoàn bao gồm các nanosheet pseudo-polyrotaxane (PPR) đơn tinh thể có độ dày bằng với độ dài PO trung tâm. Điều này là do β-CD chọn lọc bao phủ thành phần PO và gây ra sự tách pha vi mô giữa β-CD và các lớp EO. Độ dày của các lớp EO tăng tuyến tính theo số lượng đơn vị EO tăng lên, điều này cho thấy rằng các chuỗi EO bị hạn chế trong các hình trụ ảo có đường kính của β-CD. Điều này có nghĩa là chúng ta có thể kiểm soát chính xác độ dày của cả tinh thể (β-CD và PO) và các lớp vô định hình (EO) trong cấu trúc phiến. Ngược lại, EO2PO29EO2 tạo thành một cấu trúc tấm mỏng, trong đó không chỉ PO mà cả các chuỗi EO đều được bao phủ bởi β-CD. Hơn nữa, độ dài của thành phần PO trung tâm là cần thiết để hình thành cấu trúc phiến với sự tách pha giữa các lớp β-CD và EO. Những phát hiện này cung cấp một hiểu biết cơ bản hơn để nâng cao tính đa dạng và khả năng ứng dụng của các vật liệu tự lắp ráp dựa trên CD."} +{"idx": 1870233, "en": "The use of engineered nanoscale magnetic materials in healthcare and biomedical technologies is rapidly growing. Two examples which have recently attracted significant attention are magnetic particle imaging (MPI) for biological monitoring, and magnetic field hyperthermia (MFH) for cancer therapy. Here for the first time, the capability of a Lissajous scanning MPI device to act as a standalone platform to support the application of MFH cancer treatment is presented. The platform is shown to offer functionalities for nanoparticle localization, focused hyperthermia therapy application, and non-invasive tissue thermometry in one device. Combined, these capabilities have the potential to significantly enhance the accuracy, effectiveness and safety of MFH therapy. Measurements of nanoparticle hyperthermia during protracted exposure to the MPI scanner's 3D imaging field sequence revealed spatially focused heating, with a maximum that is significantly enhanced compared with a simple 1-dimensional sinusoidal excitation. The observed spatial heating behavior is qualitatively described based on a phenomenological model considering torques exerted in the Brownian regime. In vitro cell studies using a human acute monocytic leukemia cell line (THP-1) demonstrated strong suppression of both structural integrity and metabolic activity within 24 h following a 40 min MFH treatment actuated within the Lissajous MPI scanner. Furthermore, reconstructed MPI images of the nanoparticles distributed among the cells, and the temperature-sensitivity of the MPI imaging signal obtained during treatment are demonstrated. In summary, combined Lissajous MPI and MFH technologies are presented; demonstrating for the first time their potential for cancer treatment with maximum effectiveness, and minimal collateral damage to surrounding tissues.", "gemini": "Việc sử dụng các vật liệu từ tính nano được thiết kế trong công nghệ chăm sóc sức khỏe và y sinh đang phát triển nhanh chóng. Hai ví dụ gần đây đã thu hút sự chú ý đáng kể là chụp ảnh hạt từ tính (MPI) để theo dõi sinh học và tăng nhiệt độ từ trường (MFH) để điều trị ung thư. Ở đây, lần đầu tiên, khả năng của thiết bị MPI quét Lissajous hoạt động như một nền tảng độc lập để hỗ trợ ứng dụng điều trị ung thư MFH được trình bày. Nền tảng này được chứng minh là cung cấp các chức năng cho việc định vị hạt nano, ứng dụng liệu pháp tăng nhiệt tập trung và đo nhiệt độ mô không xâm lấn trong một thiết bị. Kết hợp lại, các khả năng này có tiềm năng nâng cao đáng kể độ chính xác, hiệu quả và tính an toàn của liệu pháp MFH. Các phép đo tăng nhiệt độ hạt nano trong quá trình tiếp xúc kéo dài với chuỗi trường ảnh 3D của máy quét MPI cho thấy sự gia nhiệt tập trung theo không gian, với mức tối đa được tăng cường đáng kể so với sự kích thích hình sin 1 chiều đơn giản. Hành vi gia nhiệt không gian quan sát được được mô tả định tính dựa trên mô hình hiện tượng học xem xét các mô men xoắn tác dụng trong chế độ Brown. Các nghiên cứu tế bào trong ống nghiệm sử dụng dòng tế bào bạch cầu đơn nhân cấp tính của người (THP-1) đã chứng minh sự ức chế mạnh mẽ cả tính toàn vẹn về cấu trúc và hoạt động trao đổi chất trong vòng 24 giờ sau khi điều trị MFH 40 phút được kích hoạt trong máy quét MPI Lissajous. Hơn nữa, các hình ảnh MPI tái tạo của các hạt nano phân bố giữa các tế bào và độ nhạy nhiệt độ của tín hiệu hình ảnh MPI thu được trong quá trình điều trị được chứng minh. Tóm lại, các công nghệ MPI và MFH Lissajous kết hợp được trình bày; lần đầu tiên chứng minh tiềm năng của chúng đối với việc điều trị ung thư với hiệu quả tối đa và thiệt hại tối thiểu cho các mô xung quanh."} +{"idx": 1870234, "en": "Bis-cyclometallated Ir(iii) complexes containing 2-(1H-pyrazol-3-yl)pyridine ligands have been synthesised. Their absorption is almost unchanged with changes in pH however the emission intensities vary by a factor of up to three and the complexes have emission pKas in the range 8.0 to 10.0. Substituents on the pyrazole have only a minor effect on the emission pKa. Surprisingly the complexes with phenylpyrazole cyclometallated ligands 3aL1-3 showed an intensity decrease with increasing pH (switch off) whilst the corresponding phenylpyridine ones 3cL1-3 showed an increase in emission intensity with increasing pH. Putting electron-withdrawing CF3 substituents on the cyclometallating phenyls reduced the pKa of the complexes to 6.8-7.8, thereby extending the useful pKa range; however, in general it tended to reduce the magnitude of the change in emission intensity. Surprisingly the CF3-substituted complexes also showed a complete reversal in the direction of the intensity change when compared to their respective unsubstituted congeners.", "gemini": "Các hợp chất phức Ir(iii) được liên kết cyclometallated kép chứa các phối tử 2-(1H-pyrazol-3-yl)pyridine đã được tổng hợp. Sự hấp thụ của chúng hầu như không thay đổi khi pH thay đổi, tuy nhiên cường độ phát xạ thay đổi theo hệ số lên đến ba và các hợp chất phức có pKa phát xạ trong khoảng từ 8,0 đến 10,0. Các chất thế trên pyrazol chỉ có tác động nhỏ đến pKa phát xạ. Thật ngạc nhiên là các hợp chất phức với các phối tử liên kết cyclometallated phenylpyrazol 3aL1-3 cho thấy cường độ giảm khi pH tăng (tắt) trong khi các hợp chất phenylpyridine tương ứng 3cL1-3 cho thấy cường độ phát xạ tăng khi pH tăng. Việc đặt các chất thế CF3 hút điện tử trên các phenyl liên kết cyclometallated làm giảm pKa của các hợp chất phức xuống 6,8-7,8, do đó mở rộng phạm vi pKa hữu ích; tuy nhiên, nhìn chung, nó có xu hướng làm giảm cường độ thay đổi cường độ phát xạ. Thật ngạc nhiên là các hợp chất phức được thế CF3 cũng cho thấy sự đảo ngược hoàn toàn về hướng thay đổi cường độ khi so sánh với các hợp chất tương ứng chưa được thế."} +{"idx": 1870235, "en": "Konjac glucomannan (KGM) is associated with the satiety-enhancing property by imparting the food matrix with high viscosity. In the present study, rheology tests on KGM sol with different viscosities were conducted to understand its flow behavior as they presented in the mouth and stomach, and the in vitro gastric emptying characteristics of KGM were examined with a human gastric simulator. Then, their effects on subjective appetite, glycemia, and appetite-related hormones (insulin, GLP-1, PYY3-36, CCK-8, ghrelin) response were investigated by conducting a randomized, single-blind, crossover trial in 22 healthy adults (11 female and 11 male, mean age (years): 23.2 ± 2.0, BMI (kg m-2): 20.6 ± 2.1). The blood samples and ratings for subjective appetite were collected at regular time intervals after the subjects were fed with four test breakfasts (one control treatment and three experimental treatments) on four different days. An ad libitum lunch was provided to the subjects once they consumed the breakfasts and their food intake was recorded. As the viscosity increased, the gastric emptying rate was delayed despite a large part of the chyme viscosity lost during digestion. The satiating capacity of the test breakfast was significantly enhanced as its viscosity increased, the and subjects' sensation for hunger, fullness, desire-to-eat, and prospective food consumption differed significantly (p = 0.006, 0.000, 0.002, and 0.001, respectively) between the treatments. The secretion of glycemia and satiety-related hormones were beneficially modulated by the increased viscosity of the test meal but a small decrease in the ad libitum food intake was observed after the intervention of the viscous test breakfasts. Overall, elevating the meal viscosity moderately by using KGM could contribute to combating the challenge of hunger for people in the bodyweight management.", "gemini": "Konjac glucomannan (KGM) có liên quan đến đặc tính tăng cảm giác no bằng cách truyền cho ma trận thực phẩm độ nhớt cao. Trong nghiên cứu hiện tại, các thử nghiệm lưu biến trên dung dịch KGM với độ nhớt khác nhau đã được tiến hành để hiểu hành vi chảy của nó khi chúng xuất hiện trong miệng và dạ dày, và các đặc tính làm rỗng dạ dày trong ống nghiệm của KGM đã được kiểm tra bằng mô phỏng dạ dày của người. Sau đó, tác dụng của chúng đối với cảm giác thèm ăn chủ quan, đường huyết và phản ứng của các hormone liên quan đến cảm giác thèm ăn (insulin, GLP-1, PYY3-36, CCK-8, ghrelin) đã được điều tra bằng cách tiến hành thử nghiệm chéo đơn mù ngẫu nhiên ở 22 người lớn khỏe mạnh (11 nữ và 11 nam, độ tuổi trung bình (năm): 23,2 ± 2,0, BMI (kg m-2): 20,6 ± 2,1). Các mẫu máu và xếp hạng cảm giác thèm ăn chủ quan được thu thập theo các khoảng thời gian đều đặn sau khi các đối tượng được cho ăn bốn bữa sáng thử nghiệm (một chế độ ăn đối chứng và ba chế độ ăn thử nghiệm) trong bốn ngày khác nhau. Một bữa trưa tự chọn đã được cung cấp cho các đối tượng sau khi họ ăn sáng và lượng thức ăn của họ được ghi lại. Khi độ nhớt tăng, tốc độ làm rỗng dạ dày bị chậm lại mặc dù một phần lớn độ nhớt của thức ăn bị mất trong quá trình tiêu hóa. Khả năng làm no của bữa sáng thử nghiệm được tăng cường đáng kể khi độ nhớt của nó tăng lên, và cảm giác đói, no, mong muốn ăn và dự kiến tiêu thụ thức ăn của đối tượng khác nhau đáng kể (p = 0,006, 0,000, 0,002 và 0,001, tương ứng) giữa các chế độ ăn. Quá trình tiết đường huyết và các hormone liên quan đến cảm giác no được điều chỉnh có lợi nhờ độ nhớt tăng của bữa ăn thử nghiệm nhưng lượng thức ăn tự chọn giảm một chút đã được quan sát thấy sau khi can thiệp vào các bữa sáng thử nghiệm có độ nhớt. Nhìn chung, việc tăng độ nhớt của bữa ăn ở mức độ vừa phải bằng cách sử dụng KGM có thể góp phần chống lại cơn đói cho những người trong quá trình quản lý cân nặng."} +{"idx": 1870236, "en": "We performed a resonance shear measurement (RSM) based on a low-temperature surface force apparatus to evaluate the frictional properties of the interface between butadiene rubber and ice at various temperatures below 0 °C. Friction between the rubber and ice was high and constant at temperatures below -5 °C, but sharply decreased when the temperature rose above -5 °C. We performed the same measurement by replacing the rubber with polystyrene and silica films which were rigid and exhibited practically minimal elastic deformation in comparison to the rubber. The friction decreased gradually with the increase in temperature from -20 °C to 0 °C at both the polystyrene-ice and the silica-ice interfaces. These results indicated that the elasticity of rubber was responsible for the differences in the rubber-ice interface and the other two samples. To understand the detailed mechanism of friction between the rubber and the ice, we analyzed the obtained RSM data using a physical model. The result indicated that the friction between ice and rubber was determined by the elastic deformation of the rubber film at temperatures below -5 °C, and by the viscosity of the ice premelting layer above -5 °C.", "gemini": "Chúng tôi đã thực hiện phép đo cắt cộng hưởng (RSM) dựa trên thiết bị lực bề mặt nhiệt độ thấp để đánh giá các đặc tính ma sát của giao diện giữa cao su butadien và băng ở các nhiệt độ khác nhau dưới 0 °C. Ma sát giữa cao su và băng rất cao và không đổi ở nhiệt độ dưới -5 °C, nhưng giảm mạnh khi nhiệt độ tăng trên -5 °C. Chúng tôi đã thực hiện phép đo tương tự bằng cách thay thế cao su bằng các màng polystyrene và silica cứng và thể hiện biến dạng đàn hồi tối thiểu trên thực tế so với cao su. Ma sát giảm dần khi nhiệt độ tăng từ -20 °C đến 0 °C ở cả giao diện polystyrene-băng và silica-băng. Những kết quả này chỉ ra rằng độ đàn hồi của cao su chịu trách nhiệm cho sự khác biệt giữa giao diện cao su-băng và hai mẫu còn lại. Để hiểu cơ chế chi tiết của ma sát giữa cao su và băng, chúng tôi đã phân tích dữ liệu RSM thu được bằng cách sử dụng mô hình vật lý. Kết quả chỉ ra rằng ma sát giữa băng và cao su được xác định bởi biến dạng đàn hồi của màng cao su ở nhiệt độ dưới -5 °C và bởi độ nhớt của lớp băng tan chảy trước ở trên -5 °C."} +{"idx": 1870237, "en": "As the second leading cause of blindness in the world, glaucoma is mainly caused by persistent high intraocular pressure (IOP) that compresses the optic nerve and causes permanent damage. Noninvasive continuous monitoring of IOP is an essential method for the diagnosis and treatment of glaucoma. In this paper, we propose a new strain gauge material based on graphene nanowalls (GNWs) for continuous monitoring of IOP with high sensitivity in a wide range. By simulation, we studied the relationship between the strain of the cornea and contact lens and IOP. The structure and the location of the GNWs in the contact lens are optimized. A method for transferring GNWs on contact lenses with the assistance of a gold film is proposed. The simulated tests on porcine eyes in vitro show that the resistance response of the device to normal IOP fluctuation reaches 1.014 kΩ mmHg-1. Its normal sensitivity of 42 250 ppm mmHg-1 and the response range of 0-75 mmHg are far more than those of most noninvasive methods reported before. This study shows the enormous potential of GNWs for continuous IOP monitoring with high sensitivity and low power consumption.", "gemini": "Là nguyên nhân gây mù lòa đứng thứ hai trên thế giới, bệnh tăng nhãn áp chủ yếu do áp suất nội nhãn (IOP) cao dai dẳng gây chèn ép dây thần kinh thị giác và gây tổn thương vĩnh viễn. Theo dõi liên tục IOP không xâm lấn là một phương pháp thiết yếu để chẩn đoán và điều trị bệnh tăng nhãn áp. Trong bài báo này, chúng tôi đề xuất một vật liệu ứng suất kế mới dựa trên các thành graphene (GNW) để theo dõi liên tục IOP với độ nhạy cao trong một phạm vi rộng. Bằng mô phỏng, chúng tôi đã nghiên cứu mối quan hệ giữa độ căng của giác mạc và kính áp tròng và IOP. Cấu trúc và vị trí của GNW trong kính áp tròng được tối ưu hóa. Một phương pháp chuyển GNW trên kính áp tròng với sự hỗ trợ của màng vàng được đề xuất. Các thử nghiệm mô phỏng trên mắt lợn trong ống nghiệm cho thấy phản ứng điện trở của thiết bị đối với biến động IOP bình thường đạt 1,014 kΩ mmHg-1. Độ nhạy bình thường của nó là 42 250 ppm mmHg-1 và phạm vi phản ứng từ 0-75 mmHg cao hơn nhiều so với hầu hết các phương pháp không xâm lấn đã được báo cáo trước đây. Nghiên cứu này cho thấy tiềm năng to lớn của GNW đối với việc theo dõi IOP liên tục với độ nhạy cao và mức tiêu thụ điện năng thấp."} +{"idx": 1870238, "en": "We report twisted 1T TaS2 bilayers synthesized by a lithiation exfoliation method. Atomic-scale observations reveal the existence of eight twist commensurate configurations from over 50 1T TaS2 bilayer samples in the twist angle range from 0° to 30° in which commensurate atomic configurations can be distinguished by scanning transmission electron microscopy. The limited number of twist angles, rather than random ones, indicates that there are energetically favorite twist angles in the naturally formed bilayers. Together with the interlayer distance measurements, the formation of the bilayer twist configurations is anticipated to be regulated by the stacking energy in the charge-density-wave system through interlayer van der Waals interactions. The findings of this work may pave a new way to fabricate twisted bilayer TMDs for exploring exotic properties from additional moiré periodicity.", "gemini": "Chúng tôi báo cáo các lớp kép TaS2 1T xoắn được tổng hợp bằng phương pháp tẩy lớp lithi. Các quan sát ở quy mô nguyên tử cho thấy sự tồn tại của tám cấu hình tương ứng xoắn từ hơn 50 mẫu lớp kép TaS2 1T trong phạm vi góc xoắn từ 0° đến 30° trong đó các cấu hình nguyên tử tương ứng có thể được phân biệt bằng kính hiển vi điện tử truyền qua quét. Số lượng góc xoắn giới hạn, thay vì các góc ngẫu nhiên, chỉ ra rằng có những góc xoắn yêu thích về mặt năng lượng trong các lớp kép hình thành tự nhiên. Cùng với các phép đo khoảng cách giữa các lớp, quá trình hình thành các cấu hình xoắn lớp kép được dự đoán là do năng lượng xếp chồng trong hệ sóng mật độ điện tích thông qua các tương tác van der Waals giữa các lớp. Những phát hiện của công trình này có thể mở ra một con đường mới để chế tạo TMD lớp kép xoắn để khám phá các tính chất kỳ lạ từ chu kỳ moiré bổ sung."} +{"idx": 1870239, "en": "This study examines the osteogenic differentiation promotion effect of micro/nanostructures of raffia on human adipose-derived stem cells to confirm the potential application of plant-derived micro/nanotopographies in tissue regeneration. The results confirm that the nanorod array on the front surface and the honeycomb-like microstructure on the back surface of raffia can not only regulate the adhesion, spreading, and migration of stem cells but also promote the osteogenic differentiation of the stem cells at a subsequent stage of cell culture. The osteocalcin expressions by the 21-day cultured cells on the front and back surfaces of raffia were 55-fold and 36-fold higher compared to the expression on a tissue culture plate. This indicates that plant-derived micro/nanotopographies can significantly promote stem cell differentiation. Furthermore, a general strategy for the application of plant-derived materials to stem-cell differentiation and bone-tissue engineering is suggested.", "gemini": "Nghiên cứu này kiểm tra tác dụng thúc đẩy biệt hóa tạo xương của vi cấu trúc/nanocấu trúc của raffia trên tế bào gốc lấy từ mô mỡ người để xác nhận khả năng ứng dụng tiềm năng của các vi cấu trúc/nanocấu trúc có nguồn gốc từ thực vật trong tái tạo mô. Kết quả xác nhận rằng mảng nanorod trên bề mặt trước và vi cấu trúc giống tổ ong trên bề mặt sau của raffia không chỉ có thể điều chỉnh độ bám dính, sự lan rộng và di chuyển của tế bào gốc mà còn thúc đẩy sự biệt hóa tạo xương của tế bào gốc ở giai đoạn nuôi cấy tế bào tiếp theo. Biểu hiện osteocalcin của các tế bào nuôi cấy 21 ngày trên bề mặt trước và sau của raffia cao hơn 55 lần và 36 lần so với biểu hiện trên đĩa nuôi cấy mô. Điều này chỉ ra rằng các vi cấu trúc/nanocấu trúc có nguồn gốc từ thực vật có thể thúc đẩy đáng kể sự biệt hóa của tế bào gốc. Hơn nữa, một chiến lược chung cho việc ứng dụng các vật liệu có nguồn gốc từ thực vật vào quá trình biệt hóa tế bào gốc và kỹ thuật mô xương được đề xuất."} +{"idx": 1870240, "en": "Poly(β-l-malic acid) (PMLA) together with its derivatives is an aliphatic polyester with superior bio-properties for anti-tumor drugs. In order to surmount the obstacles of low drug loading and rapid premature release during the circulation of polyester-based micelles, micelles based on poly(β-benzyl malate)-b-polyethylene glycol (PBM-PEG) were developed in this study. The micelles had high drug loading capacity (>20 wt%) and held robust stability, owing to the π-π stacking interactions between polymer chains, and between the polymer and drug. Computer simulation also confirmed that there was the strongest binding free energy between PBMs, and PBM and doxorubicin (DOX), compared with other polyesters. A cell-penetrating moiety (TAT) was employed, and furthermore, a protective outer shell (PEG5k) was also introduced via a matrix metalloproteinase-2 (MMP-2) cleavable peptide. Before reaching the tumor site, the TAT peptide was shielded by long chain PEG, and the micelles showed low bioactivity. While at the tumor tissues where MMP-2 was highly expressed, the cleavage of the linker leads to the exposure of TAT, thus enhancing the cellular internalization. The desired therapeutic consequent was also observed, with no accompanying systemic toxicity detected. Our findings indicated that this MMP-2 sensitive PBM polymeric micelle would be a promising antitumor drug carrier with enhanced therapeutic effects.", "gemini": "Poly(β-l-axit malic) (PMLA) cùng với các dẫn xuất của nó là một polyester aliphatic với các tính chất sinh học vượt trội cho các loại thuốc chống khối u. Để vượt qua những trở ngại về tải thuốc thấp và giải phóng sớm nhanh chóng trong quá trình lưu hành của các micelle gốc polyester, các micelle dựa trên poly(β-benzyl malate)-b-polyethylene glycol (PBM-PEG) đã được phát triển trong nghiên cứu này. Các micelle có khả năng tải thuốc cao (> 20 wt%) và có độ ổn định mạnh mẽ, nhờ vào các tương tác xếp chồng π-π giữa các chuỗi polyme và giữa polyme và thuốc. Mô phỏng máy tính cũng xác nhận rằng có năng lượng liên kết tự do mạnh nhất giữa các PBM và PBM và doxorubicin (DOX), so với các polyester khác. Một phần tử xâm nhập tế bào (TAT) đã được sử dụng, và hơn nữa, một lớp vỏ ngoài bảo vệ (PEG5k) cũng được đưa vào thông qua một peptide có thể cắt được bởi metalloproteinase-2 (MMP-2) của ma trận. Trước khi đến vị trí khối u, peptide TAT đã được bảo vệ bởi PEG chuỗi dài và các micelle cho thấy hoạt tính sinh học thấp. Trong khi ở các mô khối u có biểu hiện MMP-2 cao, sự cắt của liên kết dẫn đến sự tiếp xúc của TAT, do đó tăng cường nội bào hóa tế bào. Hậu quả điều trị mong muốn cũng được quan sát thấy, không phát hiện thấy độc tính toàn thân đi kèm. Những phát hiện của chúng tôi chỉ ra rằng micelle polyme PBM nhạy cảm với MMP-2 này sẽ là một chất mang thuốc chống khối u đầy hứa hẹn với hiệu quả điều trị được tăng cường."} +{"idx": 1870241, "en": "In this paper, we explore the influence of structural properties, thermal stability, and temperature on the rotational frequency of (0,n) armchair multi-walled black phosphorene nanotubes (MWβPNTs). Using Density Functional Theory (DFT) calculations, we first determine the influence of the outer wall rotation on the structural stability of (0,12)@(0,19) DWβPNTs, and (0,12)@(0,19)@(0,26) TWβPNTs. The results indicate that the relative energies of the DW- and the TWβPNTs do not change with the rotation angle. Therefore, the outer wall rotation is not important for the structural formation of the MWβPNTs. Then, using molecular dynamics (MD) simulations, we study the structural properties, thermal stability, and rotational frequency of (0,12)@(0,19) DWβPNTs, (0,12)@(0,19)@(0,26) TWβPNTs, and (0,12)@(0,19)@(0,26)@(0,33) QWβPNTs from 1 K to 400 K. The calorific curve, the mean-squared displacement, and the radial distribution function are analyzed to characterize the temperature behavior of the MWβPNTs. In all cases, the nanotubes are rotating: they act as thermal-driven rotors as the temperature increases, with a maximum rotational frequency of 16.7 GHz (clockwise direction) at 5 K for the DWβPNTs. Our results suggest that MWβPNTs could be used to construct room temperature nanomotors.", "gemini": "Trong bài báo này, chúng tôi khám phá ảnh hưởng của các tính chất cấu trúc, độ ổn định nhiệt và nhiệt độ đến tần số quay của các ống nano phốt pho đen thành nhiều lớp kiểu ghế bành (0,n) (MWβPNTs). Sử dụng các tính toán Lý thuyết hàm mật độ (DFT), trước tiên chúng tôi xác định ảnh hưởng của sự quay của thành ngoài đến độ ổn định cấu trúc của DWβPNTs (0,12)@(0,19) và TWβPNTs (0,12)@(0,19)@(0,26). Kết quả chỉ ra rằng năng lượng tương đối của DW- và TWβPNTs không thay đổi theo góc quay. Do đó, sự quay của thành ngoài không quan trọng đối với sự hình thành cấu trúc của MWβPNTs. Sau đó, sử dụng các mô phỏng động học phân tử (MD), chúng tôi nghiên cứu các tính chất cấu trúc, độ ổn định nhiệt và tần số quay của DWβPNTs (0,12)@(0,19), TWβPNTs (0,12)@(0,19)@(0,26) và QWβPNTs (0,12)@(0,19)@(0,26)@(0,33) từ 1 K đến 400 K. Đường cong lượng nhiệt, độ dịch chuyển bình phương trung bình và hàm phân bố bán kính được phân tích để mô tả đặc tính nhiệt độ của MWβPNTs. Trong mọi trường hợp, các ống nano đều quay: chúng hoạt động như các rôto dẫn động nhiệt khi nhiệt độ tăng, với tần số quay tối đa là 16,7 GHz (theo chiều kim đồng hồ) ở 5 K đối với DWβPNTs. Kết quả của chúng tôi cho thấy rằng MWβPNTs có thể được sử dụng để chế tạo các nanomô tơ nhiệt độ phòng."} +{"idx": 1870242, "en": "Embolotherapy using particle embolics is normally performed with exogenous contrast to assist in visualization. However, the exact location of the embolics cannot be identified after contrast washout. We developed a novel, pseudo-check valve-integrated microfluidic device, that partitions barium- impregnated alginate from crosslinking solution, thereby preventing nozzle failure. This enables rapid and continuous generation of inherently X-ray-visible embolic microspheres (XEMs) with uniform size. The XEMs are visible under clinical X-ray and cone beam CT both in vitro and in vivo. In particular, we demonstrated the embolization properties of these XEMs in large animals, performing direct intra- and post-procedural assessment of embolic delivery. The persistent radiopacity of these XEMs enables real-time evaluation of embolization precision and offers great promise for non-invasive follow-up examination without exogenous contrast. We also demonstrated that bariatric arterial embolization with XEMs significantly suppresses weight gain in swine, as an example of a non-oncological application of embolotherapy.", "gemini": "Thuyên tắc mạch bằng hạt thuyên tắc thường được thực hiện với độ tương phản ngoại sinh để hỗ trợ hình dung. Tuy nhiên, không thể xác định được vị trí chính xác của thuyên tắc sau khi rửa sạch độ tương phản. Chúng tôi đã phát triển một thiết bị vi lưu mới, tích hợp van kiểm tra giả, phân vùng alginate tẩm bari từ dung dịch liên kết chéo, do đó ngăn ngừa hỏng vòi phun. Điều này cho phép tạo nhanh và liên tục các vi cầu thuyên tắc có thể nhìn thấy bằng tia X (XEM) có kích thước đồng đều. Các XEM có thể nhìn thấy dưới tia X lâm sàng và CT chùm nón cả trong ống nghiệm và trong cơ thể sống. Đặc biệt, chúng tôi đã chứng minh các đặc tính thuyên tắc của các XEM này ở động vật lớn, thực hiện đánh giá trực tiếp trong và sau thủ thuật về việc đưa thuyên tắc. Độ mờ X-quang liên tục của các XEM này cho phép đánh giá thời gian thực về độ chính xác của thuyên tắc và hứa hẹn rất nhiều cho việc kiểm tra theo dõi không xâm lấn mà không cần độ tương phản ngoại sinh. Chúng tôi cũng chứng minh rằng thuyên tắc động mạch bariatric bằng XEM làm giảm đáng kể việc tăng cân ở lợn, như một ví dụ về ứng dụng không phải ung thư của liệu pháp thuyên tắc."} +{"idx": 1870243, "en": "The solvatochromic fluorophore Nile Red, 9-diethylamino-5H-benzo[a]phenoxazine-5-one, is one of the most commonly used stains to enhance contrast of lipid-rich areas of microscopic biosamples. Quite surprisingly, relatively little is known about the spectrally-resolved two-photon absorption (2PA) properties of this dye despite its promising features for two-photon microscopy of biological matter. For this reason, the two-photon solvatochromism of Nile Red still remains an uncharted territory as well. Also, no study has yet reported on how electron-withdrawing substituents attached to the Nile Red backbone affect its solvatochromic properties and two-photon brightness. In this paper, we demonstrate how solvent polarity influences the one- and two-photon absorption spectra of Nile Red as well as its fluorescence parameters, and we present new analogues that contain -CF3, -F and -Br substituents on its eastern side. Two-photon excited fluorescence experiments in a broad spectral range (780-1240 nm) and electronic structure calculations show that both the nature and location of the substituent have particular influence on the strength of 2PA, peaking in all cases at approx. 860 and 1050 nm. 2PA cross sections are higher at 1050 nm than at 860 nm, which suggests that Nile Red and its analogues are best suited for two-photon imaging employing excitation in the NIR-II optical transparency window of biological tissues.", "gemini": "Fluorophore solvatochromic Nile Red, 9-diethylamino-5H-benzo[a]phenoxazine-5-one, là một trong những chất nhuộm được sử dụng phổ biến nhất để tăng độ tương phản của các vùng giàu lipid của các mẫu sinh học dưới kính hiển vi. Đáng ngạc nhiên là, tương đối ít thông tin về các tính chất hấp thụ hai photon (2PA) được giải quyết theo phổ của thuốc nhuộm này mặc dù nó có các đặc điểm đầy hứa hẹn cho kính hiển vi hai photon của vật chất sinh học. Vì lý do này, tính solvatochromic hai photon của Nile Red vẫn là một lãnh thổ chưa được khám phá. Ngoài ra, chưa có nghiên cứu nào báo cáo về cách các chất thế rút electron gắn vào xương sống Nile Red ảnh hưởng đến các tính chất solvatochromic và độ sáng hai photon của nó. Trong bài báo này, chúng tôi trình bày cách độ phân cực của dung môi ảnh hưởng đến phổ hấp thụ một và hai photon của Nile Red cũng như các thông số huỳnh quang của nó, và chúng tôi trình bày các chất tương tự mới có chứa các chất thế -CF3, -F và -Br ở phía đông của nó. Các thí nghiệm huỳnh quang kích thích hai photon trong một dải phổ rộng (780-1240 nm) và các phép tính cấu trúc điện tử cho thấy cả bản chất và vị trí của chất thế đều có ảnh hưởng đặc biệt đến cường độ của 2PA, đạt đỉnh trong mọi trường hợp ở khoảng 860 và 1050 nm. Mặt cắt ngang 2PA cao hơn ở 1050 nm so với 860 nm, điều này cho thấy Nile Red và các chất tương tự của nó phù hợp nhất để chụp ảnh hai photon sử dụng sự kích thích trong cửa sổ trong suốt quang học NIR-II của các mô sinh học."} +{"idx": 1870244, "en": "Resistive switching devices offer a great potential for advanced computing and data storage, including neuromorphic networks and random-access memory. State-of-the-art memristors are mostly realized by a three-layer structure, which is comprised of an active metal oxide layer sandwiched between two metal electrodes. Thus, there is always an interface involving two materials differing strongly in crystallographic and electronic properties. In this study, we present a resistive switching nanorod device based on a metal oxide sandwiched between two transparent conductive oxide electrodes. Thus, the system is characterized by a different, smooth interface offering new possibilities for increased energy efficiency and transparent electronics. Antimony-doped tin oxide (ATO) is used as an electrode material. The heavily doped ATO nanorods, exhibiting a good conductivity, are produced by a templated electrochemical deposition approach of alloy particles with subsequent thermal oxidation. The process enables precise control of the doping level within the nanorods and the formation of a doping level gradient. Electrical characterization reveals that a stronger gradient between heavily doped and undoped tin oxide within the nanorods results in a more rectifying character of the junction. Three-domain nanorods consisting of an undoped tin oxide segment in between two ATO segments are utilized to introduce memristive properties into the nanorod device. The resistive switching of these nanorods can be attributed to an oxygen vacancy doping gradient introduced during thermal oxidation. These vacancies are mobile within the tin oxide host structure and their injection from the ATO segment into the undoped tin oxide segment results in altered conductivity of the device, when an external bias is applied.", "gemini": "Các thiết bị chuyển mạch điện trở mang lại tiềm năng lớn cho điện toán và lưu trữ dữ liệu tiên tiến, bao gồm mạng lưới hình thái thần kinh và bộ nhớ truy cập ngẫu nhiên. Các memristor hiện đại chủ yếu được tạo ra bởi cấu trúc ba lớp, bao gồm một lớp oxit kim loại hoạt tính được kẹp giữa hai điện cực kim loại. Do đó, luôn có một giao diện liên quan đến hai vật liệu khác nhau mạnh về các tính chất tinh thể học và điện tử. Trong nghiên cứu này, chúng tôi trình bày một thiết bị nanorod chuyển mạch điện trở dựa trên oxit kim loại được kẹp giữa hai điện cực oxit dẫn trong suốt. Do đó, hệ thống được đặc trưng bởi một giao diện nhẵn, khác biệt, mở ra khả năng mới để tăng hiệu suất năng lượng và điện tử trong suốt. Oxit thiếc pha tạp antimon (ATO) được sử dụng làm vật liệu điện cực. Các nanorod ATO pha tạp mạnh, thể hiện độ dẫn điện tốt, được sản xuất bằng phương pháp lắng đọng điện hóa có khuôn mẫu của các hạt hợp kim với quá trình oxy hóa nhiệt tiếp theo. Quá trình này cho phép kiểm soát chính xác mức độ pha tạp trong các nanorod và sự hình thành của gradient mức độ pha tạp. Đặc tính điện cho thấy rằng gradient mạnh hơn giữa oxit thiếc pha tạp mạnh và không pha tạp trong các nanorod dẫn đến đặc tính chỉnh lưu nhiều hơn của mối nối. Các nanorod ba miền bao gồm một đoạn oxit thiếc không pha tạp giữa hai đoạn ATO được sử dụng để đưa các tính chất memristor vào thiết bị nanorod. Sự chuyển mạch điện trở của các nanorod này có thể được quy cho gradient pha tạp khuyết hổng oxy được đưa vào trong quá trình oxy hóa nhiệt. Các khuyết tật này di động trong cấu trúc chủ oxit thiếc và sự tiêm chúng từ đoạn ATO vào đoạn oxit thiếc không pha tạp dẫn đến độ dẫn điện thay đổi của thiết bị khi có sự chênh lệch điện áp bên ngoài."} +{"idx": 1870245, "en": "A cascade reaction involving the Zn-catalyzed dearomatization of indoles, base-promoted ring-expansion and intramolecular SNAr reaction has been developed. This process realized a novel, atom economical and efficient synthesis of indoline-fused eight-membered azaheterocycles in a one pot manner.", "gemini": "Một phản ứng thác bao gồm khử thơm indole xúc tác bằng Zn, phản ứng mở vòng được thúc đẩy bởi bazơ và phản ứng SNAr phân tử nội đã được phát triển. Quá trình này đã thực hiện một tổng hợp mới, tiết kiệm nguyên tử và hiệu quả của azaheterocycle tám thành viên hợp nhất indoline theo cách một nồi."} +{"idx": 1870246, "en": "Covering: 2000 to 2020 Machine learning (ML) is an efficient tool for the prediction of bioactivity and the study of structure-activity relationships. Over the past decade, an emerging trend for combining these approaches with the study of natural products (NPs) has developed in order to manage the challenge of the discovery of bioactive NPs. In the present review, we will introduce the basic principles and protocols for using the ML approach to investigate the bioactivity of NPs, citing a series of practical examples regarding the study of anti-microbial, anti-cancer, and anti-inflammatory NPs, etc. ML algorithms manage a variety of classification and regression problems associated with bioactive NPs, from those that are linear to non-linear and from pure compounds to plant extracts. Inspired by cases reported in the literature and our own experience, a number of key points have been emphasized for reducing modeling errors, including dataset preparation and applicability domain analysis.", "gemini": "Bao gồm: Từ năm 2000 đến năm 2020, học máy (ML) là một công cụ hiệu quả để dự đoán hoạt tính sinh học và nghiên cứu mối quan hệ cấu trúc-hoạt động. Trong thập kỷ qua, một xu hướng mới nổi là kết hợp các phương pháp tiếp cận này với nghiên cứu về các sản phẩm tự nhiên (NP) đã phát triển để giải quyết thách thức trong việc khám phá các NP có hoạt tính sinh học. Trong bài đánh giá hiện tại, chúng tôi sẽ giới thiệu các nguyên tắc cơ bản và giao thức để sử dụng phương pháp ML để nghiên cứu hoạt tính sinh học của NP, trích dẫn một loạt các ví dụ thực tế liên quan đến nghiên cứu về NP kháng khuẩn, chống ung thư và chống viêm, v.v. Các thuật toán ML giải quyết nhiều vấn đề về phân loại và hồi quy liên quan đến NP có hoạt tính sinh học, từ các vấn đề tuyến tính đến phi tuyến tính và từ các hợp chất tinh khiết đến chiết xuất thực vật. Lấy cảm hứng từ các trường hợp được báo cáo trong tài liệu và kinh nghiệm của chúng tôi, một số điểm chính đã được nhấn mạnh để giảm lỗi mô hình, bao gồm chuẩn bị tập dữ liệu và phân tích miền khả dụng."} +{"idx": 1870247, "en": "As is known, alfalfa saponins can be used as a feed additive in a pig's diet and the addition of alfalfa saponins to a pig's diet could improve its antioxidant capacity. However, the mechanism by which alfalfa saponins exert their antioxidant effects has not been studied. To address this issue, H2O2-induced rat intestinal epithelial cells were used to establish an oxidative stress model to explore the protective mechanism of alfalfa saponins in this study. The results demonstrated that alfalfa saponins could rescue the cell proliferation activity, elevate the amount of antioxidant enzymes and downregulate the release of MDA and LDH in H2O2-induced cells. The antioxidant activity of alfalfa saponins was achieved by restoring GSH homeostasis. Further results demonstrated that alfalfa saponins could inhibit cell apoptosis through activating the MAPK signaling pathway. These results elucidated the mechanism by which alfalfa saponins exert their antioxidant effects and provided a potential strategy for alleviating oxidative stress in monogastric animals.", "gemini": "Như đã biết, saponin cỏ linh lăng có thể được sử dụng làm chất phụ gia thức ăn trong chế độ ăn của lợn và việc bổ sung saponin cỏ linh lăng vào chế độ ăn của lợn có thể cải thiện khả năng chống oxy hóa của lợn. Tuy nhiên, cơ chế mà saponin cỏ linh lăng phát huy tác dụng chống oxy hóa của chúng vẫn chưa được nghiên cứu. Để giải quyết vấn đề này, các tế bào biểu mô ruột của chuột gây ra bởi H2O2 đã được sử dụng để thiết lập một mô hình stress oxy hóa để khám phá cơ chế bảo vệ của saponin cỏ linh lăng trong nghiên cứu này. Kết quả chứng minh rằng saponin cỏ linh lăng có thể cứu hoạt động tăng sinh tế bào, nâng cao lượng enzym chống oxy hóa và giảm giải phóng MDA và LDH trong các tế bào do H2O2 gây ra. Hoạt tính chống oxy hóa của saponin cỏ linh lăng đạt được bằng cách phục hồi cân bằng nội môi GSH. Các kết quả tiếp theo chứng minh rằng saponin cỏ linh lăng có thể ức chế quá trình apoptosis tế bào thông qua kích hoạt con đường truyền tín hiệu MAPK. Những kết quả này làm sáng tỏ cơ chế mà saponin cỏ linh lăng phát huy tác dụng chống oxy hóa của chúng và cung cấp một chiến lược tiềm năng để giảm stress oxy hóa ở động vật đơn dạ dày."} +{"idx": 1870248, "en": "Malaria continues to be a global health threat, affecting approximately 219 million people in 2018 alone. The recurrent development of resistance to existing antimalarials means that the design of new drug candidates must be carefully considered. Understanding of drug target mechanism can dramatically accelerate early-stage target-based development of novel antimalarials and allows for structural modifications even during late-stage preclinical development. Here, we have provided an overview of three promising antimalarial molecular targets, PfDHFR, PfDHODH and PfA-M1, and their associated inhibitors which demonstrate how mechanism can inform drug design and be effectively utilised to generate compounds with potent inhibitory activity.", "gemini": "Sốt rét tiếp tục là mối đe dọa sức khỏe toàn cầu, chỉ riêng năm 2018 đã ảnh hưởng đến khoảng 219 triệu người. Sự phát triển tái phát của tình trạng kháng thuốc đối với các thuốc chống sốt rét hiện có có nghĩa là việc thiết kế các ứng cử viên thuốc mới phải được cân nhắc cẩn thận. Hiểu biết về cơ chế mục tiêu thuốc có thể thúc đẩy đáng kể quá trình phát triển dựa trên mục tiêu ở giai đoạn đầu của thuốc chống sốt rét mới và cho phép sửa đổi cấu trúc ngay cả trong quá trình phát triển tiền lâm sàng giai đoạn cuối. Tại đây, chúng tôi đã cung cấp tổng quan về ba mục tiêu phân tử chống sốt rét đầy hứa hẹn, PfDHFR, PfDHODH và PfA-M1, và các chất ức chế liên quan của chúng, chứng minh cách cơ chế có thể cung cấp thông tin cho thiết kế thuốc và được sử dụng hiệu quả để tạo ra các hợp chất có hoạt tính ức chế mạnh."} +{"idx": 1870249, "en": "Host plant volatiles play a key role in mediating plant-herbivore interactions. How an array of host plant volatiles guides host preference and attraction in the invasive polyphagous Lycorma delicatula (White), the spotted lanternfly (SLF), is largely unknown. A pernicious phloem feeder, SLF feeds on over 70 species of plants, some with high economic impact. To aid the development of detection and monitoring tools for SLF, we used a two-choice olfactometer to compare 14 host plant species for attraction, first to a blank control, and then to their preferred host Ailanthus altissima (Mill.) Swingle (Sapindales: Simaroubaceae), tree-of-heaven. SLF were significantly attracted to seven host plants compared to a blank control, but no host plant was more attractive than tree-of-heaven. We then used electroantennographic detection (EAD) to screen select host plants for EAD active compounds, hypothesizing that EAD-active plant volatiles act as kairomones and mediate SLF attraction to host plants. Out of 43 unique antennal responses, 18 compounds were identified and tested individually for attraction in a two-choice olfactometer against a blank control and then against methyl salicylate, the current best attractant. Eleven compounds were significantly attractive, and one, sulcatone, was more attractive than methyl salicylate. Blends of kairomones were then tested for attraction, revealing five blends that were significantly more attractive than methyl salicylate, and could be developed into lures for field testing. The presence of these kairomones in volatile profiles of 17 plant species is described. These findings support the hypothesis that the identified volatiles act as kairomones and function in attraction to host plants.", "gemini": "Các chất dễ bay hơi của cây chủ đóng vai trò chính trong việc trung gian các tương tác giữa cây và động vật ăn cỏ. Cách mà một loạt các chất dễ bay hơi của cây chủ hướng dẫn sở thích và sức hấp dẫn của vật chủ ở loài Lycorma delicatula (White) ăn tạp xâm lấn, loài bọ đèn lồng đốm (SLF), phần lớn vẫn chưa được biết rõ. Là loài ăn rệp mạch pernicious, SLF ăn hơn 70 loài thực vật, một số loài có tác động kinh tế cao. Để hỗ trợ phát triển các công cụ phát hiện và giám sát SLF, chúng tôi đã sử dụng máy đo khứu giác hai lựa chọn để so sánh 14 loài thực vật chủ về sức hấp dẫn, đầu tiên là so với đối chứng trống, sau đó là so với cây chủ ưa thích của chúng là Ailanthus altissima (Mill.) Swingle (Sapindales: Simaroubaceae), cây thiên đường. SLF bị hấp dẫn đáng kể bởi bảy loài thực vật chủ so với đối chứng trống, nhưng không có loài thực vật chủ nào hấp dẫn hơn cây thiên đường. Sau đó, chúng tôi đã sử dụng phương pháp phát hiện điện tử (EAD) để sàng lọc các loài thực vật chủ được chọn để tìm các hợp chất hoạt động của EAD, giả thuyết rằng các chất dễ bay hơi của thực vật hoạt động với EAD hoạt động như kairomone và trung gian cho sức hấp dẫn của SLF đối với cây chủ. Trong số 43 phản ứng ăng-ten riêng biệt, 18 hợp chất đã được xác định và thử nghiệm riêng lẻ về sức hấp dẫn trong máy đo khứu giác hai lựa chọn so với đối chứng trống và sau đó so với methyl salicylat, chất dẫn dụ tốt nhất hiện tại. Mười một hợp chất có sức hấp dẫn đáng kể và một hợp chất, sulcatone, hấp dẫn hơn methyl salicylat. Các hỗn hợp kairomone sau đó đã được thử nghiệm về sức hấp dẫn, cho thấy năm hỗn hợp hấp dẫn hơn đáng kể so với methyl salicylat và có thể được phát triển thành mồi nhử để thử nghiệm ngoài thực địa. Sự hiện diện của các kairomone này trong các cấu hình dễ bay hơi của 17 loài thực vật được mô tả. Những phát hiện này hỗ trợ cho giả thuyết rằng các chất dễ bay hơi được xác định hoạt động như kairomone và có chức năng hấp dẫn đối với cây chủ."} +{"idx": 1870250, "en": "Atherosclerosis, the pathology underlying heart attacks, strokes and peripheral artery disease, is a chronic inflammatory disease of the artery wall initiated by elevated low-density lipoprotein (LDL) cholesterol levels. LDL accumulates in the artery wall, where it can become oxidized to oxLDL. T cell responses to ApoB, a core protein found in LDL and other lipoproteins, are detectable in healthy mice and people. Most of the ApoB-specific CD4T cells are FoxP3+ regulatory T cells (Treg). In the course of atherosclerosis development, the number of ApoB-reactive T cells expands. At the same time, their phenotype changes, showing cell surface markers, transcription factors and transcriptomes resembling other T-helper lineages like Th17, Th1 and follicular helper (TFH) cells. TFH cells enter germinal centers and provide T cell help to B cells, enabling antibody isotype switch from IgM to IgG and supporting affinity maturation. In people and mice with atherosclerosis, IgG and IgM antibodies to oxLDL are detectable. Higher IgM antibody titers to oxLDL are associated with less, IgG antibodies with more atherosclerosis. Thus, both T and B cells play critical roles in atherosclerosis. Modifying the adaptive immune response to ApoB holds promise for preventing atherosclerosis and reducing disease burden.", "gemini": "Xơ vữa động mạch, bệnh lý nền tảng gây ra các cơn đau tim, đột quỵ và bệnh động mạch ngoại vi, là một bệnh viêm mạn tính của thành động mạch khởi phát do nồng độ cholesterol lipoprotein tỷ trọng thấp (LDL) tăng cao. LDL tích tụ ở thành động mạch, tại đó nó có thể bị oxy hóa thành oxLDL. Phản ứng tế bào T đối với ApoB, một protein cốt lõi có trong LDL và các lipoprotein khác, có thể phát hiện được ở chuột và người khỏe mạnh. Hầu hết các tế bào CD4T đặc hiệu ApoB là tế bào T điều hòa FoxP3+ (Treg). Trong quá trình phát triển xơ vữa động mạch, số lượng tế bào T phản ứng với ApoB tăng lên. Đồng thời, kiểu hình của chúng thay đổi, biểu hiện các dấu hiệu bề mặt tế bào, các yếu tố phiên mã và phiên mã giống như các dòng tế bào T hỗ trợ khác như tế bào Th17, Th1 và tế bào hỗ trợ nang (TFH). Các tế bào TFH xâm nhập vào các trung tâm mầm và cung cấp sự hỗ trợ của tế bào T cho các tế bào B, cho phép chuyển đổi isotype kháng thể từ IgM sang IgG và hỗ trợ sự trưởng thành về ái lực. Ở những người và chuột bị xơ vữa động mạch, có thể phát hiện thấy kháng thể IgG và IgM đối với oxLDL. Nồng độ kháng thể IgM cao hơn đối với oxLDL có liên quan đến ít xơ vữa động mạch hơn, trong khi kháng thể IgG có liên quan đến nhiều xơ vữa động mạch hơn. Do đó, cả tế bào T và tế bào B đều đóng vai trò quan trọng trong xơ vữa động mạch. Việc điều chỉnh phản ứng miễn dịch thích ứng với ApoB hứa hẹn sẽ giúp ngăn ngừa xơ vữa động mạch và giảm gánh nặng bệnh tật."} +{"idx": 1870251, "en": "Modifications of the cellular proteome pool upon stress allow plants to tolerate environmental changes. Alternative splicing is the most significant mechanism responsible for the production of multiple protein isoforms from a single gene. The spliceosome, a large ribonucleoprotein complex, together with several associated proteins, controls this pre-mRNA processing, adding an additional level of regulation to gene expression. Deep sequencing of transcriptomes revealed that this co- or post-transcriptional mechanism is highly induced by abiotic stress, and concerns vast numbers of stress-related genes. Confirming the importance of splicing in plant stress adaptation, key players of stress signaling have been shown to encode alternative transcripts, whereas mutants lacking splicing factors or associated components show a modified sensitivity and defective responses to abiotic stress. Here, we examine recent literature on alternative splicing and splicing alterations in response to environmental stresses, focusing on its role in stress adaptation and analyzing the future perspectives and directions for research.", "gemini": "Các sửa đổi của nhóm proteome tế bào khi bị căng thẳng cho phép cây chịu được những thay đổi của môi trường. Ghép nối thay thế là cơ chế quan trọng nhất chịu trách nhiệm sản xuất nhiều dạng protein từ một gen. Spliceosome, một phức hợp ribonucleoprotein lớn, cùng với một số protein liên quan, kiểm soát quá trình xử lý tiền mRNA này, bổ sung thêm một cấp độ điều hòa cho biểu hiện gen. Trình tự sâu của phiên mã cho thấy cơ chế đồng hoặc sau phiên mã này được cảm ứng rất cao bởi căng thẳng phi sinh học và liên quan đến số lượng lớn các gen liên quan đến căng thẳng. Xác nhận tầm quan trọng của quá trình ghép nối trong thích nghi với căng thẳng của cây, các tác nhân chính của tín hiệu căng thẳng đã được chứng minh là mã hóa các bản phiên mã thay thế, trong khi các đột biến thiếu các yếu tố ghép nối hoặc các thành phần liên quan biểu hiện độ nhạy được sửa đổi và phản ứng khiếm khuyết với căng thẳng phi sinh học. Ở đây, chúng tôi xem xét các tài liệu gần đây về ghép nối thay thế và các thay đổi ghép nối để đáp ứng với các căng thẳng của môi trường, tập trung vào vai trò của nó trong thích ứng với căng thẳng và phân tích các quan điểm và hướng nghiên cứu trong tương lai."} +{"idx": 1870252, "en": "Spotted-wing drosophila (Drosophila suzukii Matsumura) is a major pest of soft-skinned fruit and due to the low infestation tolerance for marketable fruit, growers take preventive actions to hinder spotted-wing drosophila damages. Insecticides application is one of the measures taken by growers. Although intensive spraying programs have been used to manage spotted-wing drosophila, its early infestation, rapid reproduction, and vast range of host have caused damage to still occur in fruit, including tart cherries, Prunus ceraus (Linnaeus). Therefore, there is a merit for information on insecticide's curative activity to understand whether sprays manage spotted-wing drosophila individuals within infested fruit. Tart cherry fruit were exposed to spotted-wing drosophila adults for 3 d. After this infestation period, insecticides were applied 1 and 3 d later. Small larvae, large larvae, and pupae were counted 9 d after initial infestation. A parallel set of insecticide-treated tart cherries were subjected to residue analysis. Phosmet and spinetoram were able to reduced live spotted-wing drosophila counts compared with the control at all life stages and insecticide application times, whereas zeta-cypermethrin, acetamiprid, and cyantraniliprole were less consistent in reducing spotted-wing drosophila numbers. Chromobacterium subtsugae demonstrated no curative action. Residue analysis demonstrated that zeta-cypermethrin residues mostly remained on fruit surface. Small portions of phosmet, spinetoram, and cyantraniliprole were able to penetrate fruit surfaces and move into subsurface tissues. Acetamiprid was the only compound which >47% penetrated into the fruit subsurface consistently across both years. Curative activity demonstrated in this study can provide additional tactics for spotted-wing drosophila management in tart cherry Integrated Pest Management (IPM) programs.", "gemini": "Ruồi giấm cánh đốm (Drosophila suzukii Matsumura) là một loài gây hại chính đối với trái cây vỏ mềm và do ngưỡng chịu nhiễm thấp đối với trái cây có thể bán được, người trồng thực hiện các biện pháp phòng ngừa để ngăn chặn thiệt hại do ruồi giấm cánh đốm gây ra. Việc sử dụng thuốc trừ sâu là một trong những biện pháp mà người trồng thực hiện. Mặc dù các chương trình phun thuốc chuyên sâu đã được sử dụng để kiểm soát ruồi giấm cánh đốm, nhưng sự xâm nhiễm sớm, sinh sản nhanh và phạm vi ký chủ rộng của nó đã gây ra thiệt hại vẫn xảy ra đối với trái cây, bao gồm cả anh đào chua, Prunus ceraus (Linnaeus). Do đó, cần có thông tin về hoạt động chữa bệnh của thuốc trừ sâu để hiểu xem thuốc phun có kiểm soát được ruồi giấm cánh đốm trong trái cây bị nhiễm hay không. Trái anh đào chua đã tiếp xúc với ruồi giấm cánh đốm trưởng thành trong 3 ngày. Sau thời gian nhiễm này, thuốc trừ sâu được phun sau 1 và 3 ngày. Ấu trùng nhỏ, ấu trùng lớn và nhộng được đếm sau 9 ngày kể từ khi nhiễm ban đầu. Một bộ anh đào chua được xử lý bằng thuốc trừ sâu song song đã được phân tích dư lượng. Phosmet và spinetoram có khả năng giảm số lượng ruồi giấm cánh đốm sống so với đối chứng ở tất cả các giai đoạn sống và thời điểm phun thuốc trừ sâu, trong khi zeta-cypermethrin, acetamiprid và cyantraniliprole ít nhất quán hơn trong việc giảm số lượng ruồi giấm cánh đốm. Chromobacterium subtsugae không biểu hiện tác dụng chữa bệnh. Phân tích dư lượng cho thấy phần lớn dư lượng zeta-cypermethrin vẫn còn trên bề mặt quả. Một phần nhỏ phosmet, spinetoram và cyantraniliprole có thể thâm nhập vào bề mặt quả và di chuyển vào các mô dưới bề mặt. Acetamiprid là hợp chất duy nhất mà > 47% thâm nhập vào dưới bề mặt quả một cách nhất quán trong cả hai năm. Hoạt động chữa bệnh được chứng minh trong nghiên cứu này có thể cung cấp các chiến thuật bổ sung để quản lý ruồi giấm cánh đốm trong các chương trình Quản lý dịch hại tổng hợp (IPM) của anh đào chua."} +{"idx": 1870253, "en": "Cardiac fibrosis is a key physiological response to cardiac tissue injury to protect the heart from wall rupture. However, its progression increases heart stiffness, eventually causing a decrease in heart contractility. Unfortunately, to date, no efficient antifibrotic therapies are available to the clinic. This is primarily due to the complexity of the process, which involves several cell types and signaling pathways. For instance, the transforming growth factor beta (TGF-β) signaling pathway has been recognized to be vital for myofibroblasts activation and fibrosis progression. In this context, complex sphingolipids, such as ganglioside GM3, have been shown to be directly involved in TGF-β receptor 1 (TGF-R1) activation. In this work, we report that an induced up-regulation of sialidase Neu3, a glycohydrolytic enzyme involved in ganglioside cell homeostasis, can significantly reduce cardiac fibrosis in primary cultures of human cardiac fibroblasts by inhibiting the TGF-β signaling pathway, ultimately decreasing collagen I deposition. These results support the notion that modulating ganglioside GM3 cell content could represent a novel therapeutic approach for cardiac fibrosis, warranting for further investigations.", "gemini": "Xơ hóa tim là phản ứng sinh lý chính đối với tổn thương mô tim để bảo vệ tim khỏi vỡ thành. Tuy nhiên, sự tiến triển của nó làm tăng độ cứng của tim, cuối cùng dẫn đến giảm khả năng co bóp của tim. Thật không may, cho đến nay, không có liệu pháp chống xơ hóa hiệu quả nào có sẵn tại phòng khám. Điều này chủ yếu là do sự phức tạp của quá trình, liên quan đến nhiều loại tế bào và các con đường truyền tín hiệu. Ví dụ, con đường truyền tín hiệu của yếu tố tăng trưởng chuyển đổi beta (TGF-β) đã được công nhận là rất quan trọng đối với quá trình hoạt hóa nguyên bào sợi cơ và tiến triển xơ hóa. Trong bối cảnh này, các sphingolipid phức tạp, chẳng hạn như ganglioside GM3, đã được chứng minh là có liên quan trực tiếp đến sự hoạt hóa thụ thể TGF-β 1 (TGF-R1). Trong nghiên cứu này, chúng tôi báo cáo rằng sự điều hòa tăng lên của sialidase Neu3, một loại enzyme thủy phân glyco liên quan đến cân bằng tế bào ganglioside, có thể làm giảm đáng kể xơ hóa tim trong nuôi cấy nguyên bào sợi tim người chính bằng cách ức chế con đường truyền tín hiệu TGF-β, cuối cùng làm giảm sự lắng đọng collagen I. Những kết quả này hỗ trợ cho quan điểm cho rằng việc điều chỉnh hàm lượng tế bào ganglioside GM3 có thể đại diện cho một phương pháp điều trị mới cho bệnh xơ hóa tim, đảm bảo cho các cuộc điều tra sâu hơn."} +{"idx": 1870254, "en": "Extracellular vesicles (EVs), membrane-bound vesicles that are naturally released by cells, have emerged as new therapeutic opportunities. EVs, particularly exosomes and microvesicles, can transfer effector molecules and elicit potent responses in recipient cells, making them attractive therapeutic targets and drug delivery platforms. Furthermore, containing predictive biomarkers and often being dysregulated in various disease settings, these EVs are being exploited for diagnostic purposes. In contrast, the therapeutic application of apoptotic bodies (ApoBDs), a distinct type of EVs released by cells undergoing a form of programmed cell death called apoptosis, has been largely unexplored. Recent studies have shed light on ApoBD biogenesis and functions, promisingly implicating their therapeutic potential. In this review, we discuss many strategies to develop ApoBD-based therapies as well as highlight their advantages and challenges, thereby positioning ApoBD for potential EV-based therapy.", "gemini": "Các túi ngoại bào (EV), các túi liên kết màng được giải phóng tự nhiên bởi các tế bào, đã nổi lên như những cơ hội điều trị mới. EV, đặc biệt là exosome và vi túi, có thể truyền các phân tử hiệu ứng và gây ra phản ứng mạnh ở các tế bào nhận, khiến chúng trở thành mục tiêu điều trị hấp dẫn và nền tảng phân phối thuốc. Hơn nữa, chứa các dấu ấn sinh học dự đoán và thường bị rối loạn trong các bệnh cảnh khác nhau, các EV này đang được khai thác cho mục đích chẩn đoán. Ngược lại, ứng dụng điều trị của các thể apoptotic (ApoBD), một loại EV riêng biệt được giải phóng bởi các tế bào trải qua một dạng chết tế bào theo chương trình được gọi là apoptosis, phần lớn vẫn chưa được khám phá. Các nghiên cứu gần đây đã làm sáng tỏ quá trình sinh học và chức năng của ApoBD, hứa hẹn cho thấy tiềm năng điều trị của chúng. Trong bài đánh giá này, chúng tôi thảo luận về nhiều chiến lược để phát triển các liệu pháp dựa trên ApoBD cũng như nêu bật những ưu điểm và thách thức của chúng, do đó định vị ApoBD cho liệu pháp tiềm năng dựa trên EV."} +{"idx": 1870255, "en": "Ovarian cancer is the most lethal diseases among women. The chemo-resistance has been a big challenge for the cancer treatment. It has been reported that metformin may inhibit ovarian cancer and is able to impede the development of drug resistance, but the molecular mechanisms remain elusive. In this study, we explored the molecular roles of metformin in Parkin expression and p53 ubiquitination in chemo-resistant ovarian cancer cells. Firstly, ovarian cancer and chemo-resistant ovarian cancer cells were selected for determining the expression of Parkin, p53, and p53 signaling pathway-related factors. Then the cell proliferation and viability after loss- and gain-of-function assays were measured. Besides, immunoprecipitation (IP) was used to determine the interactions between Parkin and p53, and the ubiquitination level of p53 was measured using in vitro ubiquitination assay. Finally, the degradation of p53 proteasome regulated by Parkin was monitored using the MG132 proteasome inhibitor. We found that metformin significantly inhibited the growth of ovarian cancer parental cells and chemo-resistant cells, and metformin promoted Parkin expression in chemo-resistant cells. Further, up-regulated Parkin expression promoted the ubiquitination and degradation of p53, and metformin inhibited the expression of p53 to suppress the proliferation of chemo-resistant ovarian cancer cells. Mechanistically, metformin could inhibit the growth of ovarian cancer cells by promoting the Parkin-induced p53 ubiquitination. Altogether, our study demonstrated an inhibitory role of metformin in the growth of chemo-resistant cancer cells through promoting the Parkin-induced p53 ubiquitination, which provides a novel mechanism of metformin for treating ovarian cancer.", "gemini": "Ung thư buồng trứng là căn bệnh gây tử vong nhiều nhất ở phụ nữ. Kháng hóa chất là một thách thức lớn đối với việc điều trị ung thư. Người ta đã báo cáo rằng metformin có thể ức chế ung thư buồng trứng và có khả năng ngăn cản sự phát triển của tình trạng kháng thuốc, nhưng cơ chế phân tử vẫn còn khó nắm bắt. Trong nghiên cứu này, chúng tôi đã khám phá vai trò phân tử của metformin trong biểu hiện Parkin và ubiquitin hóa p53 trong các tế bào ung thư buồng trứng kháng hóa chất. Đầu tiên, các tế bào ung thư buồng trứng và các tế bào ung thư buồng trứng kháng hóa chất đã được lựa chọn để xác định biểu hiện của Parkin, p53 và các yếu tố liên quan đến con đường truyền tín hiệu p53. Sau đó, sự tăng sinh và khả năng sống của tế bào sau các xét nghiệm mất và tăng chức năng đã được đo. Bên cạnh đó, kết tủa miễn dịch (IP) được sử dụng để xác định các tương tác giữa Parkin và p53, và mức độ ubiquitin hóa của p53 được đo bằng xét nghiệm ubiquitin hóa trong ống nghiệm. Cuối cùng, sự thoái hóa của proteasome p53 được Parkin điều chỉnh đã được theo dõi bằng cách sử dụng chất ức chế proteasome MG132. Chúng tôi phát hiện ra rằng metformin ức chế đáng kể sự phát triển của các tế bào gốc ung thư buồng trứng và các tế bào kháng hóa chất, và metformin thúc đẩy biểu hiện Parkin trong các tế bào kháng hóa chất. Hơn nữa, biểu hiện Parkin được điều chỉnh lên thúc đẩy quá trình ubiquitin hóa và thoái hóa của p53, và metformin ức chế biểu hiện của p53 để ức chế sự tăng sinh của các tế bào ung thư buồng trứng kháng hóa chất. Về mặt cơ chế, metformin có thể ức chế sự phát triển của các tế bào ung thư buồng trứng bằng cách thúc đẩy quá trình ubiquitin hóa p53 do Parkin gây ra. Nhìn chung, nghiên cứu của chúng tôi đã chứng minh vai trò ức chế của metformin trong sự phát triển của các tế bào ung thư kháng hóa chất thông qua việc thúc đẩy quá trình ubiquitin hóa p53 do Parkin gây ra, cung cấp một cơ chế mới của metformin để điều trị ung thư buồng trứng."} +{"idx": 1870256, "en": "RAS is a membrane localized small GTPase frequently mutated in human cancer. As such, RAS has been a focal target for developing cancer therapeutics since its discovery nearly four decades ago. However, efforts to directly target RAS have been challenging due to the apparent lack of readily discernable deep pockets for binding small molecule inhibitors leading many to consider RAS as undruggable. An important milestone in direct RAS inhibition was achieved recently with the groundbreaking discovery of covalent inhibitors that target the mutant Cys residue in KRAS(G12C). Surprisingly, these G12C-reactive compounds only target mutant RAS in the GDP-bound state thereby locking it in the inactive conformation and blocking its ability to couple with downstream effector pathways. Building on this success, several groups have developed similar compounds that selectively target KRAS(G12C), with AMG510 and MRTX849 the first to advance to clinical trials. Both have shown early promising results. Though the success with these compounds has reignited the possibility of direct pharmacological inhibition of RAS, these covalent inhibitors are limited to treating KRAS(G12C) tumors which account for <15% of all RAS mutants in human tumors. Thus, there remains an unmet need to identify more broadly efficacious RAS inhibitors. Here, we will discuss the current state of RAS(G12C) inhibitors and the potential for inhibiting additional RAS mutants through targeting RAS dimerization which has emerged as an important step in the allosteric regulation of RAS function.", "gemini": "RAS là một GTPase nhỏ được định vị trên màng thường đột biến ở ung thư ở người. Do đó, RAS đã trở thành mục tiêu trọng tâm để phát triển liệu pháp điều trị ung thư kể từ khi được phát hiện gần bốn thập kỷ trước. Tuy nhiên, những nỗ lực nhắm trực tiếp vào RAS đã gặp nhiều thách thức do dường như thiếu các túi sâu dễ nhận biết để liên kết với các chất ức chế phân tử nhỏ khiến nhiều người coi RAS là không thể dùng thuốc. Một cột mốc quan trọng trong việc ức chế RAS trực tiếp đã đạt được gần đây với khám phá đột phá về các chất ức chế cộng hóa trị nhắm vào phần Cys đột biến trong KRAS(G12C). Đáng ngạc nhiên, các hợp chất phản ứng với G12C này chỉ nhắm vào RAS đột biến ở trạng thái liên kết GDP, do đó khóa nó ở dạng không hoạt động và ngăn chặn khả năng kết hợp với các con đường hiệu ứng hạ nguồn. Dựa trên thành công này, một số nhóm đã phát triển các hợp chất tương tự nhắm chọn lọc vào KRAS(G12C), với AMG510 và MRTX849 là những hợp chất đầu tiên được đưa vào thử nghiệm lâm sàng. Cả hai đều cho thấy những kết quả ban đầu đầy hứa hẹn. Mặc dù thành công với các hợp chất này đã làm sống lại khả năng ức chế dược lý trực tiếp RAS, nhưng các chất ức chế cộng hóa trị này chỉ giới hạn trong việc điều trị các khối u KRAS(G12C) chiếm <15% trong số tất cả các đột biến RAS ở các khối u ở người. Do đó, vẫn còn nhu cầu chưa được đáp ứng là xác định các chất ức chế RAS hiệu quả hơn. Ở đây, chúng tôi sẽ thảo luận về tình trạng hiện tại của các chất ức chế RAS(G12C) và tiềm năng ức chế các đột biến RAS bổ sung thông qua việc nhắm mục tiêu vào quá trình dimer hóa RAS, một bước quan trọng trong quá trình điều hòa dị lập của chức năng RAS."} +{"idx": 1870257, "en": "Among the major challenges in the development of biopharmaceuticals are structural heterogeneity and aggregation. The development of a successful therapeutic monoclonal antibody (mAb) requires both a highly active and also stable molecule. Whilst a range of experimental (biophysical) approaches exist to track changes in stability of proteins, routine prediction of stability remains challenging. The fluorescence red edge excitation shift (REES) phenomenon is sensitive to a range of changes in protein structure. Based on recent work, we have found that quantifying the REES effect is extremely sensitive to changes in protein conformational state and dynamics. Given the extreme sensitivity, potentially this tool could provide a 'fingerprint' of the structure and stability of a protein. Such a tool would be useful in the discovery and development of biopharamceuticals and so we have explored our hypothesis with a panel of therapeutic mAbs. We demonstrate that the quantified REES data show remarkable sensitivity, being able to discern between structurally identical antibodies and showing sensitivity to unfolding and aggregation. The approach works across a broad concentration range (µg-mg/ml) and is highly consistent. We show that the approach can be applied alongside traditional characterisation testing within the context of a forced degradation study (FDS). Most importantly, we demonstrate the approach is able to predict the stability of mAbs both in the short (hours), medium (days) and long-term (months). The quantified REES data will find immediate use in the biopharmaceutical industry in quality assurance, formulation and development. The approach benefits from low technical complexity, is rapid and uses instrumentation which exists in most biochemistry laboratories without modification.", "gemini": "Trong số những thách thức lớn trong quá trình phát triển các sản phẩm sinh học là tính không đồng nhất về cấu trúc và sự kết tập. Việc phát triển một kháng thể đơn dòng (mAb) điều trị thành công đòi hỏi cả một phân tử hoạt động cao và ổn định. Trong khi có nhiều phương pháp tiếp cận thực nghiệm (sinh lý học) để theo dõi những thay đổi về độ ổn định của protein, thì việc dự đoán độ ổn định thường xuyên vẫn còn nhiều thách thức. Hiện tượng dịch chuyển kích thích cạnh đỏ huỳnh quang (REES) nhạy cảm với một loạt các thay đổi về cấu trúc protein. Dựa trên công trình gần đây, chúng tôi phát hiện ra rằng việc định lượng hiệu ứng REES cực kỳ nhạy cảm với những thay đổi về trạng thái và động lực cấu hình của protein. Với độ nhạy cực cao, công cụ này có khả năng cung cấp 'dấu vân tay' về cấu trúc và độ ổn định của protein. Một công cụ như vậy sẽ hữu ích trong việc khám phá và phát triển các sản phẩm sinh học và do đó chúng tôi đã khám phá giả thuyết của mình với một nhóm các mAb điều trị. Chúng tôi chứng minh rằng dữ liệu REES được định lượng cho thấy độ nhạy đáng chú ý, có khả năng phân biệt giữa các kháng thể giống hệt về mặt cấu trúc và cho thấy độ nhạy đối với sự mở ra và kết tập. Phương pháp này hoạt động trong phạm vi nồng độ rộng (µg-mg/ml) và có độ nhất quán cao. Chúng tôi chỉ ra rằng phương pháp này có thể được áp dụng cùng với thử nghiệm đặc tính truyền thống trong bối cảnh nghiên cứu phân hủy cưỡng bức (FDS). Quan trọng nhất, chúng tôi chứng minh rằng phương pháp này có khả năng dự đoán độ ổn định của mAb trong cả thời gian ngắn (giờ), trung bình (ngày) và dài hạn (tháng). Dữ liệu REES được định lượng sẽ được sử dụng ngay trong ngành dược phẩm sinh học để đảm bảo chất lượng, xây dựng công thức và phát triển. Phương pháp này được hưởng lợi từ độ phức tạp kỹ thuật thấp, nhanh chóng và sử dụng các thiết bị có sẵn trong hầu hết các phòng thí nghiệm sinh hóa mà không cần sửa đổi."} +{"idx": 1870258, "en": "Structured cis-regulatory RNAs have evolved across all domains of life, highlighting the utility and plasticity of RNA as a regulatory molecule. Homologous RNA sequences and structures often have similar functions, but homology may also be deceiving. The challenges that derive from trying to assign function to structure and vice versa are not trivial. Bacterial riboswitches, viral and eukaryotic IRESes, CITEs, and 3' UTR elements employ an array of mechanisms to exert their effects. Bioinformatic searches coupled with biochemical and functional validation have elucidated some shared and many unique ways cis-regulators are employed in mRNA transcripts. As cis-regulatory RNAs are resolved in greater detail, it is increasingly apparent that shared homology can mask the full spectrum of mRNA cis-regulator functional diversity. Furthermore, similar functions may be obscured by lack of obvious sequence similarity. Thus looking beyond homology is crucial for furthering our understanding of RNA-based regulation.", "gemini": "Các RNA điều hòa cis có cấu trúc đã tiến hóa trong mọi lĩnh vực của sự sống, làm nổi bật tiện ích và tính dẻo của RNA như một phân tử điều hòa. Trình tự và cấu trúc RNA đồng dạng thường có chức năng tương tự, nhưng tính đồng dạng cũng có thể gây hiểu lầm. Những thách thức bắt nguồn từ việc cố gắng gán chức năng cho cấu trúc và ngược lại không phải là tầm thường. Riboswitch của vi khuẩn, IRES của vi-rút và eukaryote, CITE và các yếu tố 3' UTR sử dụng một loạt các cơ chế để phát huy tác dụng của chúng. Các tìm kiếm tin sinh học kết hợp với xác thực chức năng và sinh hóa đã làm sáng tỏ một số cách thức chung và nhiều cách thức độc đáo mà các chất điều hòa cis được sử dụng trong các bản sao mRNA. Khi các RNA điều hòa cis được giải quyết chi tiết hơn, ngày càng rõ ràng rằng tính đồng dạng chung có thể che lấp toàn bộ phổ chức năng điều hòa cis của mRNA. Hơn nữa, các chức năng tương tự có thể bị che khuất do thiếu sự giống nhau rõ ràng về trình tự. Do đó, việc nhìn xa hơn tính đồng dạng là rất quan trọng để thúc đẩy sự hiểu biết của chúng ta về quy định dựa trên RNA."} +{"idx": 1870259, "en": "Automated image analysis methods have shown potential for replicating expert interpretation of histology and endoscopy images, which traditionally require highly specialized and experienced reviewers. Inflammatory bowel disease (IBD) diagnosis, severity assessment, and treatment decision-making require multimodal expert data interpretation and integration, which could be significantly aided by applications of machine learning analyses. This review introduces fundamental concepts of machine learning for imaging analysis and highlights research and development of automated histology and endoscopy interpretation in IBD. Proof-of-concept studies strongly suggest that histologic and endoscopic images can be interpreted with similar accuracy as knowledge experts. Encouraging results support the potential of automating existing disease activity scoring instruments with high reproducibility, speed, and accessibility, therefore improving the standardization of IBD assessment. Though challenges surrounding ground truth definitions, technical barriers, and the need for extensive multicenter evaluation must be resolved before clinical implementation, automated image analysis is likely to both improve access to standardized IBD assessment and advance the fundamental concepts of how disease is measured.", "gemini": "Các phương pháp phân tích hình ảnh tự động đã cho thấy tiềm năng trong việc sao chép diễn giải chuyên gia về hình ảnh mô học và nội soi, theo truyền thống đòi hỏi những người đánh giá có trình độ chuyên môn cao và giàu kinh nghiệm. Chẩn đoán bệnh viêm ruột (IBD), đánh giá mức độ nghiêm trọng và ra quyết định điều trị đòi hỏi phải diễn giải và tích hợp dữ liệu chuyên gia đa phương thức, có thể được hỗ trợ đáng kể bằng các ứng dụng phân tích học máy. Đánh giá này giới thiệu các khái niệm cơ bản về học máy để phân tích hình ảnh và nêu bật nghiên cứu và phát triển về giải thích mô học và nội soi tự động trong IBD. Các nghiên cứu bằng chứng về khái niệm cho thấy mạnh mẽ rằng hình ảnh mô học và nội soi có thể được diễn giải với độ chính xác tương tự như các chuyên gia kiến thức. Những kết quả đáng khích lệ hỗ trợ tiềm năng tự động hóa các công cụ chấm điểm hoạt động của bệnh hiện có với khả năng tái tạo cao, tốc độ và khả năng truy cập, do đó cải thiện tiêu chuẩn hóa đánh giá IBD. Mặc dù những thách thức xung quanh định nghĩa chân lý cơ bản, rào cản kỹ thuật và nhu cầu đánh giá đa trung tâm mở rộng phải được giải quyết trước khi triển khai lâm sàng, nhưng phân tích hình ảnh tự động có khả năng cải thiện khả năng tiếp cận đánh giá IBD tiêu chuẩn hóa và thúc đẩy các khái niệm cơ bản về cách thức đo lường bệnh."} +{"idx": 1870260, "en": "Geminiviruses constitute one of the largest families of plant viruses and they infect many economically important crops. The proteins encoded by the single-stranded DNA genome of these viruses interact with a wide range of host proteins to cause global dysregulation of cellular processes and help establish infection in the host. Geminiviruses have evolved numerous mechanisms to exploit host epigenetic processes to ensure the replication and survival of the viral genome. Here, we review our current knowledge of diverse epigenetic processes that have been implicated in the regulation of geminivirus pathogenesis, including DNA methylation, histone post-transcriptional modification, chromatin remodelling, and nucleosome repositioning. In addition, we discuss the currently limited evidence of host epigenetic defence responses that are aimed at counteracting geminivirus infection, and the potential for exploiting these responses for the generation of resistance against geminiviruses in crop species.", "gemini": "Geminivirus tạo nên một trong những họ virus thực vật lớn nhất và chúng lây nhiễm cho nhiều loại cây trồng quan trọng về mặt kinh tế. Các protein được mã hóa bởi bộ gen DNA sợi đơn của các loại virus này tương tác với nhiều loại protein của vật chủ để gây ra tình trạng rối loạn toàn cầu các quá trình của tế bào và giúp thiết lập tình trạng nhiễm trùng ở vật chủ. Geminivirus đã tiến hóa nhiều cơ chế để khai thác các quá trình biểu sinh của vật chủ nhằm đảm bảo sự sao chép và tồn tại của bộ gen virus. Tại đây, chúng tôi xem xét kiến thức hiện tại của chúng tôi về các quá trình biểu sinh đa dạng đã được chứng minh là có liên quan đến việc điều chỉnh sinh bệnh học của geminivirus, bao gồm quá trình methyl hóa DNA, sửa đổi sau phiên mã histone, tái cấu trúc chromatin và định vị lại nucleosome. Ngoài ra, chúng tôi thảo luận về bằng chứng hiện tại còn hạn chế về các phản ứng phòng vệ biểu sinh của vật chủ nhằm chống lại nhiễm trùng geminivirus và tiềm năng khai thác các phản ứng này để tạo ra khả năng kháng geminivirus ở các loài cây trồng."} +{"idx": 1870261, "en": "CONTEXT A comprehensive characterization of racial/ethnic variations in vitamin D metabolism markers may improve our understanding of differences in bone and mineral homeostasis and the risk of vitamin D-related diseases. OBJECTIVE Describe racial/ethnic differences in vitamin D metabolism markers and their associations with genetic ancestry. DESIGN, SETTING, PARTICIPANTS In a cross-sectional study within the Multi-Ethnic Study of Atherosclerosis (MESA), we compared a comprehensive panel of vitamin D metabolism markers across self-reported racial/ethnic groups of Black (N = 1759), White (N = 2507), Chinese (N = 788), and Hispanic (N = 1411). We evaluated associations of proportion African and European ancestry with this panel of markers in Black and Hispanic participants using ancestry informative markers. Latent class analysis evaluated associations between patterns of vitamin D measurements with race/ethnicity. RESULTS Compared with Black participants, White participants had significantly higher serum concentrations of 25-hydroxyvitamin D and fibroblast growth factor-23; lower concentrations of parathyroid hormone and 1,25-dihydroxyvitamin D; circulating vitamin D metabolite ratios suggesting lower CYP27B1 and higher CYP24A1 activity; higher urinary concentrations of calcium and phosphorus with higher urinary fractional excretion of phosphorus; and differences in vitamin D binding globulin haplotypes. Higher percent European ancestry was associated with higher 25-hydroxyvitamin D and lower parathyroid hormone concentrations among Black and Hispanic participants. Latent classes defined by vitamin D measurements reflected these patterns and differed significantly by race/ethnicity and ancestry. CONCLUSIONS Markers of vitamin D metabolism vary significantly by race/ethnicity, may serve to maintain bone and mineral homeostasis across ranges of 25-hydroxyvitamin D production, and be attributable, at least partly, to genetic ancestry.", "gemini": "NGỮ CẢNH Việc mô tả toàn diện về sự thay đổi theo chủng tộc/dân tộc trong các dấu hiệu chuyển hóa vitamin D có thể cải thiện sự hiểu biết của chúng ta về sự khác biệt trong cân bằng xương và khoáng chất cũng như nguy cơ mắc các bệnh liên quan đến vitamin D. MỤC TIÊU Mô tả sự khác biệt về chủng tộc/dân tộc trong các dấu hiệu chuyển hóa vitamin D và mối liên quan của chúng với nguồn gốc di truyền. THIẾT KẾ, BỐI CẢNH, NGƯỜI THAM GIA Trong một nghiên cứu cắt ngang trong Nghiên cứu đa dân tộc về xơ vữa động mạch (MESA), chúng tôi đã so sánh một bảng toàn diện các dấu hiệu chuyển hóa vitamin D trên các nhóm chủng tộc/dân tộc tự báo cáo là người da đen (N = 1759), người da trắng (N = 2507), người Trung Quốc (N = 788) và người gốc Tây Ban Nha (N = 1411). Chúng tôi đã đánh giá mối liên quan của tỷ lệ tổ tiên Châu Phi và Châu Âu với bảng các dấu hiệu này ở những người tham gia là người da đen và gốc Tây Ban Nha bằng cách sử dụng các dấu hiệu thông tin về tổ tiên. Phân tích lớp tiềm ẩn đánh giá mối liên quan giữa các kiểu đo lường vitamin D với chủng tộc/dân tộc. KẾT QUẢ So với những người tham gia là người da đen, những người tham gia là người da trắng có nồng độ 25-hydroxyvitamin D và yếu tố tăng trưởng nguyên bào sợi-23 trong huyết thanh cao hơn đáng kể; nồng độ hormone tuyến cận giáp và 1,25-dihydroxyvitamin D thấp hơn; tỷ lệ chuyển hóa vitamin D lưu hành cho thấy hoạt động CYP27B1 thấp hơn và hoạt động CYP24A1 cao hơn; nồng độ canxi và phốt pho trong nước tiểu cao hơn với tỷ lệ bài tiết phốt pho trong nước tiểu cao hơn; và sự khác biệt về kiểu gen globulin gắn vitamin D. Tỷ lệ phần trăm tổ tiên Châu Âu cao hơn có liên quan đến nồng độ 25-hydroxyvitamin D cao hơn và nồng độ hormone tuyến cận giáp thấp hơn ở những người tham gia là người da đen và gốc Tây Ban Nha. Các lớp tiềm ẩn được xác định bằng các phép đo vitamin D phản ánh các kiểu này và khác nhau đáng kể theo chủng tộc/dân tộc và nguồn gốc. KẾT LUẬN Các dấu hiệu chuyển hóa vitamin D thay đổi đáng kể theo chủng tộc/dân tộc, có thể giúp duy trì cân bằng xương và khoáng chất trong phạm vi sản xuất 25-hydroxyvitamin D và có thể là do ít nhất một phần là do nguồn gốc di truyền."} +{"idx": 1870262, "en": "CONTEXT Prenatal dexamethasone (DEX) treatment is sometimes used in pregnancies at risk for congenital adrenal hyperplasia (CAH) to prevent virilization in female fetuses with CAH. In boys and in fetuses not having CAH, there is no benefit of early DEX treatment and the risks of this therapy must be thoroughly investigated. High doses of prenatal glucocorticoid might alter the developmental trajectory of the brain into adulthood, even for CAH unaffected subjects treated with DEX for a short term during the first trimester. OBJECTIVE The present study investigated brain activation during working memory performance in DEX-treated subjects compared with controls. DESIGN, SETTING, AND PARTICIPANTS We tested 18 participants who were exposed to DEX during the first trimester of fetal life but did not have CAH (8 females; mean age 20.78 [standard deviation (SD), 2.67] years) and 40 control participants (24 females; mean age 20.53 [SD, 2.64]) from a single research institute. Participants underwent functional magnetic resonance imaging on a 3T scanner during a verbal and visuospatial working memory task. RESULTS We did not observe any differences in brain activity during working memory performance. However, DEX-treated subjects responded faster during the experimental condition of the verbal WM task. CONCLUSIONS First trimester DEX treatment did not seem to result in altered working memory-related brain activity at adult age. Our findings contribute to the risk-benefit assessment of prenatal DEX treatment in the context of CAH.", "gemini": "NGỮ CẢNH Đôi khi người ta sử dụng phương pháp điều trị dexamethasone (DEX) trước khi sinh ở những thai kỳ có nguy cơ tăng sản thượng thận bẩm sinh (CAH) để ngăn ngừa sự nam tính hóa ở thai nhi nữ mắc CAH. Ở trẻ trai và thai nhi không mắc CAH, việc điều trị DEX sớm không có lợi ích gì và những rủi ro của liệu pháp này phải được điều tra kỹ lưỡng. Liều lượng cao glucocorticoid trước khi sinh có thể làm thay đổi quá trình phát triển của não cho đến tuổi trưởng thành, ngay cả đối với những đối tượng không mắc CAH được điều trị bằng DEX trong thời gian ngắn trong tam cá nguyệt đầu tiên. MỤC TIÊU Nghiên cứu hiện tại đã điều tra hoạt động của não trong quá trình thực hiện trí nhớ làm việc ở những đối tượng được điều trị bằng DEX so với nhóm đối chứng. THIẾT KẾ, BỐI CẢNH VÀ NGƯỜI THAM GIA Chúng tôi đã kiểm tra 18 người tham gia tiếp xúc với DEX trong tam cá nguyệt đầu tiên của thai kỳ nhưng không mắc CAH (8 nữ; độ tuổi trung bình 20,78 [độ lệch chuẩn (SD), 2,67] tuổi) và 40 người tham gia nhóm đối chứng (24 nữ; độ tuổi trung bình 20,53 [SD, 2,64]) từ một viện nghiên cứu duy nhất. Những người tham gia đã trải qua chụp cộng hưởng từ chức năng trên máy quét 3T trong quá trình thực hiện nhiệm vụ trí nhớ làm việc bằng lời nói và thị giác không gian. KẾT QUẢ Chúng tôi không quan sát thấy bất kỳ sự khác biệt nào về hoạt động của não trong quá trình thực hiện trí nhớ làm việc. Tuy nhiên, những đối tượng được điều trị bằng DEX phản ứng nhanh hơn trong điều kiện thực nghiệm của nhiệm vụ WM bằng lời nói. KẾT LUẬN Việc điều trị bằng DEX trong tam cá nguyệt đầu tiên dường như không dẫn đến thay đổi hoạt động của não liên quan đến trí nhớ làm việc ở tuổi trưởng thành. Những phát hiện của chúng tôi góp phần vào việc đánh giá rủi ro-lợi ích của việc điều trị DEX trước khi sinh trong bối cảnh CAH."} +{"idx": 1870263, "en": "Low expression of tumor suppressor microRNA (miRNA) and high expression of carcinogenic miRNA promote the occurrence and progression of human cancer. Most studies show that miR-145 is a tumor suppressor miRNA, and is closely related to the clinicopathology of breast cancer. However, the results are still inconsistent. Therefore, we conducted a meta-analysis on the basis of eligible studies to summarize the possible correlation between miR-145 and the clinicopathology and prognosis of breast cancer. Using PubMed, Embase, Web of Science, Wanfang and CNKI, we searched all published papers written in either English or Chinese on miR-145 expression in breast cancer from 1990 to November 2019 for meta-analysis. We used standardized mean difference (SMD) to evaluate the differential expression of miR-145 in breast cancer tissues and adjacent normal tissues or normal breast tissues. We found that miR-145 expression was significantly lower in breast cancer tissues than that in adjacent normal tissues (SMD = -2.93, P<0.0001) and in healthy women (SMD = -0.52, P=0.009). miR-145 expression was lower in breast cancer patients with ER-positive (SMD = 0.65, P<0.001), HER-2-positive (SMD = -1.04, P<0.001), compared with their counterparts, respectively. In addition, breast cancer patients with low expression of miR-145 had larger tumor diameters (SMD = -1.97, P<0.001) and lymph node metastasis (SMD = -1.75, P<0.001) that are unfavorable prognostic factors. Conclusion: Low miR-145 is observed in breast cancer, which is closely related to molecular subtypes and unfavorable factors of breast cancer. These findings indicate that miR-145 is tumor suppressor miRNA, and may be a potential diagnostic and prognostic marker in breast cancer.", "gemini": "Sự biểu hiện thấp của microRNA (miRNA) ức chế khối u và sự biểu hiện cao của miRNA gây ung thư thúc đẩy sự xuất hiện và tiến triển của ung thư ở người. Hầu hết các nghiên cứu đều chỉ ra rằng miR-145 là một miRNA ức chế khối u và có liên quan chặt chẽ đến lâm sàng bệnh học của ung thư vú. Tuy nhiên, các kết quả vẫn chưa nhất quán. Do đó, chúng tôi đã tiến hành phân tích tổng hợp trên cơ sở các nghiên cứu đủ điều kiện để tóm tắt mối tương quan có thể có giữa miR-145 và lâm sàng bệnh học và tiên lượng của ung thư vú. Sử dụng PubMed, Embase, Web of Science, Wanfang và CNKI, chúng tôi đã tìm kiếm tất cả các bài báo đã công bố bằng tiếng Anh hoặc tiếng Trung về biểu hiện miR-145 trong ung thư vú từ năm 1990 đến tháng 11 năm 2019 để phân tích tổng hợp. Chúng tôi đã sử dụng chênh lệch trung bình chuẩn hóa (SMD) để đánh giá sự biểu hiện khác biệt của miR-145 trong các mô ung thư vú và các mô bình thường kề cận hoặc các mô vú bình thường. Chúng tôi thấy rằng biểu hiện miR-145 thấp hơn đáng kể trong các mô ung thư vú so với các mô bình thường kề cận (SMD = -2,93, P < 0,0001) và ở phụ nữ khỏe mạnh (SMD = -0,52, P = 0,009). Biểu hiện miR-145 thấp hơn ở những bệnh nhân ung thư vú có ER dương (SMD = 0,65, P < 0,001), HER-2 dương (SMD = -1,04, P < 0,001) so với các đối tác tương ứng của họ. Ngoài ra, những bệnh nhân ung thư vú có biểu hiện miR-145 thấp có đường kính khối u lớn hơn (SMD = -1,97, P < 0,001) và di căn hạch bạch huyết (SMD = -1,75, P < 0,001) là những yếu tố tiên lượng không thuận lợi. Kết luận: miR-145 thấp được quan sát thấy ở ung thư vú, có liên quan chặt chẽ đến các phân nhóm phân tử và các yếu tố không thuận lợi của ung thư vú. Những phát hiện này chỉ ra rằng miR-145 là miRNA ức chế khối u và có thể là một dấu hiệu chẩn đoán và tiên lượng tiềm năng trong ung thư vú."} +{"idx": 1870264, "en": "The azalea lace bug (Stephanitis pyrioides Scott) (Hemiptera: Tingidae) is an invasive pest of rhododendrons and azaleas (Ericaceae: Rhododendron), which feeds on the underside of leaves causing chlorosis, reduced photosynthesis, and even plant death. While insecticides can control this pest, growers, landscape managers, and homeowners have requested softer alternatives. Augmentative release of predatory green lacewing Chrysoperla sp. (Neuroptera: Chrysopidae) eggs and larvae has reduced S. pyrioides, but large-scale implementation may not be practical nor cost-effective. Attracting naturally occurring Chrysopidae with plant volatiles may be an economical and convenient option. In this study, we tested whether volatile blends 1) attracted Chrysoperla sp., and 2) controlled S. pyrioides populations on Rhododendron spp. in farm or urban landscapes. Experimental plots contained different multicomponent lures placed aboveground next to infested plants. Adult Chrysoperla sp., other natural enemies, and S. pyrioides from egg to adult stages were monitored in both farm and urban landscapes for two summers. Overall, two out of three volatile blends consistently attracted Chrysoperla sp. to sticky traps near baited plants. Methyl salicylate + acetic acid + 2-phenylethanol (methyl salicylate blend) and acetophenone + acetic acid + 2-phenylethanol (acetophenone blend) captured more adult Chrysoperla sp. than control traps in farm landscapes. However, only the acetophenone blend was associated with a slight reduction of S. pyrioides. Additional research is needed to determine whether the phenology of the first generation of both species are synchronized for effective season biological control in the Pacific Northwest.", "gemini": "Bọ ren azalea (Stephanitis pyrioides Scott) (Hemiptera: Tingidae) là một loài gây hại xâm lấn đối với đỗ quyên và hoa trà (Ericaceae: Rhododendron), chúng ăn mặt dưới của lá gây bệnh vàng lá, quang hợp giảm và thậm chí cả cây chết. Mặc dù thuốc trừ sâu có thể kiểm soát loài gây hại này, nhưng người trồng, người quản lý cảnh quan và chủ nhà đã yêu cầu các biện pháp thay thế nhẹ nhàng hơn. Việc phát triển trứng và ấu trùng của loài bọ cánh gân xanh ăn thịt Chrysoperla sp. (Neuroptera: Chrysopidae) đã làm giảm S. pyrioides, nhưng việc triển khai trên quy mô lớn có thể không thực tế hoặc không hiệu quả về chi phí. Thu hút loài Chrysopidae có nguồn gốc tự nhiên bằng chất dễ bay hơi của thực vật có thể là một lựa chọn kinh tế và tiện lợi. Trong nghiên cứu này, chúng tôi đã kiểm tra xem hỗn hợp dễ bay hơi có 1) thu hút Chrysoperla sp. hay không và 2) kiểm soát quần thể S. pyrioides trên Rhododendron spp. trong cảnh quan nông trại hoặc đô thị. Các ô thí nghiệm chứa các loại mồi nhiều thành phần khác nhau được đặt trên mặt đất bên cạnh các cây bị nhiễm. Bọ cánh gân xanh trưởng thành Chrysoperla sp., các loài thiên địch khác và S. pyrioides từ giai đoạn trứng đến trưởng thành đã được theo dõi ở cả cảnh quan nông trại và đô thị trong hai mùa hè. Nhìn chung, hai trong số ba hỗn hợp dễ bay hơi liên tục thu hút Chrysoperla sp. vào các bẫy dính gần cây mồi. Methyl salicylat + axit axetic + 2-phenylethanol (hỗn hợp methyl salicylat) và acetophenone + axit axetic + 2-phenylethanol (hỗn hợp acetophenone) đã bắt được nhiều bọ cánh gân xanh trưởng thành Chrysoperla sp. hơn bẫy đối chứng trong cảnh quan nông trại. Tuy nhiên, chỉ có hỗn hợp acetophenone có liên quan đến việc giảm nhẹ S. pyrioides. Cần có thêm nghiên cứu để xác định xem vật lý của thế hệ đầu tiên của cả hai loài có được đồng bộ hóa để kiểm soát sinh học theo mùa hiệu quả ở Tây Bắc Thái Bình Dương hay không."} +{"idx": 1870265, "en": "Human serine protease inhibitors (serpins) are the main inhibitors of serine proteases, but some of them also have the capability to effectively inhibit cysteine proteases. Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) main protease (Mpro) is a chymotrypsin-type cysteine protease that is needed to produce functional proteins essential for virus replication and transcription. Serpin traps its target proteases by presenting a reactive center loop (RCL) as protease-specific cleavage site, resulting in protease inactivation. Mpro target sites with its active site serine and other flanking residues can possibly interact with serpins. Alternatively, RCL cleavage site of serpins with known evidence of inhibition of cysteine proteases can be replaced by Mpro target site to make chimeric proteins. Purified chimeric serpin can possibly inhibit Mpro that can be assessed indirectly by observing the decrease in ability of Mpro to cleave its chromogenic substrate. Chimeric serpins with best interaction and active site binding and with ability to form 1:1 serpin-Mpro complex in human plasma can be assessed by using SDS/PAGE and Western blot analysis with serpin antibody. Trapping SARS-CoV-2 Mpro cysteine protease using cross-class serpin cysteine protease inhibition activity is a novel idea with significant therapeutic potential.", "gemini": "Chất ức chế protease serin ở người (serpin) là chất ức chế chính của protease serin, nhưng một số trong số chúng cũng có khả năng ức chế hiệu quả protease cystein. Protease chính của hội chứng hô hấp cấp tính nặng do virus corona 2 (SARS-CoV-2) (Mpro) là một protease cystein loại chymotrypsin cần thiết để sản xuất các protein chức năng thiết yếu cho quá trình sao chép và phiên mã của vi-rút. Serpin giữ chặt các protease mục tiêu của nó bằng cách trình bày một vòng lặp trung tâm phản ứng (RCL) như vị trí cắt đặc hiệu của protease, dẫn đến việc protease bị bất hoạt. Các vị trí mục tiêu của Mpro với serin hoạt động và các dư lượng bên sườn khác có thể tương tác với serpins. Ngoài ra, vị trí cắt RCL của serpins có bằng chứng đã biết về việc ức chế protease cystein có thể được thay thế bằng vị trí mục tiêu của Mpro để tạo ra protein chimeric. Serpin chimeric tinh khiết có khả năng ức chế Mpro có thể được đánh giá gián tiếp bằng cách quan sát thấy sự giảm khả năng của Mpro trong việc cắt cơ chất sắc tố của nó. Các serpins chimeric có sự tương tác và liên kết vị trí hoạt động tốt nhất và có khả năng tạo phức hợp serpin-Mpro 1:1 trong huyết tương người có thể được đánh giá bằng cách sử dụng phân tích SDS/PAGE và Western blot với kháng thể serpin. Việc giữ chặt protease cystein Mpro của SARS-CoV-2 bằng cách sử dụng hoạt động ức chế protease cystein serpin chéo lớp là một ý tưởng mới có tiềm năng điều trị đáng kể."} +{"idx": 1870266, "en": "Predicted increases in atmospheric concentration of carbon dioxide (CO2 ) coupled with increased temperatures and drought are expected to strongly influence the development of most of the plant species in the world, especially in areas with high risk of desertification like the Mediterranean basin. Helianthemum almeriense is an ecologically important Mediterranean shrub with an added interest because it serves as the host for the Terfezia claveryi mycorrhizal fungus, which is a desert truffle with increasingly commercial interest. Although both plant and fungi are known to be well adapted to dry conditions, it is still uncertain how the increase in atmospheric CO2 will influence them. In this article we have addressed the physiological responses of H. almeriense × T. claveryi mycorrhizal plants to increases in atmospheric CO2 coupled with drought and high vapor pressure deficit. This work reports one of the few estimations of mesophyll conductance in a drought deciduous Mediterranean shrub and evaluates its role in photosynthesis limitation. High atmospheric CO2 concentrations help desert truffle mycorrhizal plants to cope with the adverse effects of progressive drought during Mediterranean springs by improving carbon net assimilation, intrinsic water use efficiency and dispersal of the species through increased flowering events.", "gemini": "Dự đoán sự gia tăng nồng độ carbon dioxide (CO2) trong khí quyển cùng với nhiệt độ tăng và hạn hán dự kiến sẽ ảnh hưởng mạnh đến sự phát triển của hầu hết các loài thực vật trên thế giới, đặc biệt là ở những khu vực có nguy cơ sa mạc hóa cao như lưu vực Địa Trung Hải. Helianthemum almeriense là một loài cây bụi Địa Trung Hải quan trọng về mặt sinh thái với một sở thích được thêm vào vì nó đóng vai trò là vật chủ cho nấm rễ Terfezia claveryi, một loại nấm cục sa mạc ngày càng được quan tâm về mặt thương mại. Mặc dù cả thực vật và nấm đều được biết là thích nghi tốt với điều kiện khô hạn, nhưng vẫn chưa chắc chắn về việc gia tăng CO2 trong khí quyển sẽ ảnh hưởng đến chúng như thế nào. Trong bài viết này, chúng tôi đã giải quyết các phản ứng sinh lý của cây cộng sinh H. almeriense × T. claveryi đối với sự gia tăng CO2 trong khí quyển cùng với hạn hán và thâm hụt áp suất hơi cao. Công trình này báo cáo một trong số ít ước tính về độ dẫn khí trung mô ở một loài cây bụi rụng lá Địa Trung Hải chịu hạn và đánh giá vai trò của nó trong việc hạn chế quang hợp. Nồng độ CO2 cao trong khí quyển giúp các loài thực vật cộng sinh với nấm cục sa mạc đối phó với những tác động bất lợi của hạn hán tiến triển trong mùa xuân Địa Trung Hải bằng cách cải thiện quá trình đồng hóa carbon ròng, hiệu quả sử dụng nước nội tại và sự phân tán của các loài thông qua các sự kiện ra hoa tăng lên."} +{"idx": 1870267, "en": "In recent years, several genes have been implicated in the variable disease presentation of global developmental delay (GDD) and intellectual disability (ID). The endoplasmic reticulum membrane protein complex (EMC) family is known to be involved in GDD and ID. Homozygous variants of EMC1 are associated with GDD, scoliosis, and cerebellar atrophy, indicating the relevance of this pathway for neurogenetic disorders. EMC10 is a bone marrow-derived angiogenic growth factor that plays an important role in infarct vascularization and promoting tissue repair. However, this gene has not been previously associated with human disease. Herein, we describe a Saudi family with two individuals segregating a recessive neurodevelopmental disorder. Both of the affected individuals showed mild ID, speech delay, and GDD. Whole-exome sequencing (WES) and Sanger sequencing were performed to identify candidate genes. Further, to elucidate the functional effects of the variant, quantitative real-time PCR (RT-qPCR)-based expression analysis was performed. WES revealed a homozygous splice acceptor site variant (c.679-1G>A) in EMC10 (chromosome 19q13.33) that segregated perfectly within the family. RT-qPCR showed a substantial decrease in the relative EMC10 gene expression in the patients, indicating the pathogenicity of the identified variant. For the first time in the literature, the EMC10 gene variant was associated with mild ID, speech delay, and GDD. Thus, this gene plays a key role in developmental milestones, with the potential to cause neurodevelopmental disorders in humans.", "gemini": "Trong những năm gần đây, một số gen đã được xác định liên quan đến biểu hiện bệnh khác nhau của tình trạng chậm phát triển toàn diện (GDD) và khuyết tật trí tuệ (ID). Họ protein màng lưới nội chất (EMC) được biết là liên quan đến GDD và ID. Các biến thể đồng hợp tử của EMC1 có liên quan đến GDD, vẹo cột sống và teo tiểu não, cho thấy sự liên quan của con đường này đối với các rối loạn thần kinh di truyền. EMC10 là yếu tố tăng trưởng sinh mạch có nguồn gốc từ tủy xương đóng vai trò quan trọng trong quá trình mạch máu hóa nhồi máu và thúc đẩy sửa chữa mô. Tuy nhiên, gen này trước đây chưa được xác định liên quan đến bệnh ở người. Trong bài báo này, chúng tôi mô tả một gia đình ở Saudi Arabia có hai cá nhân phân ly một rối loạn phát triển thần kinh lặn. Cả hai cá nhân bị ảnh hưởng đều biểu hiện ID nhẹ, chậm nói và GDD. Trình tự toàn bộ exome (WES) và trình tự Sanger đã được thực hiện để xác định các gen ứng viên. Hơn nữa, để làm sáng tỏ các tác động chức năng của biến thể, phân tích biểu hiện dựa trên PCR định lượng thời gian thực (RT-qPCR) đã được thực hiện. WES phát hiện ra một biến thể vị trí chấp nhận ghép nối đồng hợp tử (c.679-1G>A) trong EMC10 (nhiễm sắc thể 19q13.33) phân ly hoàn hảo trong gia đình. RT-qPCR cho thấy sự giảm đáng kể trong biểu hiện gen EMC10 tương đối ở những bệnh nhân, chỉ ra tính gây bệnh của biến thể đã xác định. Lần đầu tiên trong tài liệu, biến thể gen EMC10 có liên quan đến ID nhẹ, chậm nói và GDD. Do đó, gen này đóng vai trò quan trọng trong các mốc phát triển, có khả năng gây ra các rối loạn phát triển thần kinh ở người."} +{"idx": 1870268, "en": "In the last decade, there has been a tremendous development of technologies focused on analysing various molecular attributes in single cells, with an ever-increasing number of parameters becoming available at the DNA, RNA and protein levels. Much of this progress has involved cells in suspension, but also in situ analysis of tissues has taken great leaps. Paralleling the development in the laboratory, and because of increasing complexity, the analysis of single-cell data is also constantly being updated with new algorithms and analysis platforms. Our immune system shares this complexity, and immunologists have therefore been in the forefront of this technological development. These technologies clearly open new avenues for immunology research, maybe particularly within autoimmunity where the interaction between the faulty immune system and the thymus or the target organ is important. However, the technologies currently available can seem overwhelming and daunting. The aim of this review is to remedy this by giving a balanced overview of the prospects of using single-cell analysis in basal and clinical autoimmunity research, with an emphasis on endocrine autoimmunity.", "gemini": "Trong thập kỷ qua, đã có một sự phát triển to lớn của các công nghệ tập trung vào việc phân tích các thuộc tính phân tử khác nhau trong các tế bào đơn lẻ, với số lượng thông số ngày càng tăng ở cấp độ DNA, RNA và protein. Phần lớn tiến bộ này liên quan đến các tế bào trong hệ thống treo, nhưng việc phân tích mô tại chỗ cũng đã có những bước tiến lớn. Song song với sự phát triển trong phòng thí nghiệm và do tính phức tạp ngày càng tăng, việc phân tích dữ liệu tế bào đơn cũng liên tục được cập nhật với các thuật toán và nền tảng phân tích mới. Hệ thống miễn dịch của chúng ta có chung sự phức tạp này, do đó, các nhà miễn dịch học đã đi đầu trong sự phát triển công nghệ này. Các công nghệ này rõ ràng mở ra những con đường mới cho nghiên cứu miễn dịch học, có thể đặc biệt trong trường hợp tự miễn dịch, nơi tương tác giữa hệ thống miễn dịch bị lỗi và tuyến ức hoặc cơ quan đích rất quan trọng. Tuy nhiên, các công nghệ hiện có thể có vẻ quá sức và đáng sợ. Mục đích của bài đánh giá này là khắc phục điều này bằng cách đưa ra cái nhìn tổng quan cân bằng về triển vọng sử dụng phân tích tế bào đơn trong nghiên cứu tự miễn dịch cơ bản và lâm sàng, với trọng tâm là tự miễn dịch nội tiết."} +{"idx": 1870269, "en": "Despite the importance of members of the GPCR superfamily as targets of a broad range of effective medicines many GPCRs remain poorly characterised. GPR84 is an example. Expression of GPR84 is strongly up regulated in immune cells in a range of pro-inflammatory settings and clinical trials to treat idiopathic pulmonary fibrosis are currently ongoing using ligands with differing levels of selectivity and affinity as GPR84 antagonists. Although blockade of GPR84 may potentially prove effective also in diseases associated with inflammation of the lower gut there is emerging interest in defining if agonists of GPR84 might find utility in conditions in which regulation of metabolism or energy sensing is compromised. Here, we consider the physiological and pathological expression profile of GPR84 and, in the absence of direct structural information, recent developments and use of GPR84 pharmacological tool compounds to study its broader role and biology.", "gemini": "Mặc dù tầm quan trọng của các thành viên trong siêu họ GPCR là mục tiêu của nhiều loại thuốc hiệu quả, nhưng nhiều GPCR vẫn chưa được mô tả rõ. GPR84 là một ví dụ. Sự biểu hiện của GPR84 được điều hòa mạnh mẽ ở các tế bào miễn dịch trong một loạt các điều kiện tiền viêm và các thử nghiệm lâm sàng để điều trị xơ phổi vô căn hiện đang được tiến hành bằng cách sử dụng các phối tử có mức độ chọn lọc và ái lực khác nhau như các chất đối kháng GPR84. Mặc dù việc phong tỏa GPR84 có khả năng chứng minh hiệu quả trong các bệnh liên quan đến tình trạng viêm ở ruột dưới, nhưng sự quan tâm đang nổi lên trong việc xác định xem các chất chủ vận của GPR84 có thể hữu ích trong các tình trạng mà quá trình điều chỉnh chuyển hóa hoặc cảm biến năng lượng bị tổn hại hay không. Ở đây, chúng tôi xem xét hồ sơ biểu hiện sinh lý bệnh của GPR84 và khi không có thông tin cấu trúc trực tiếp, những phát triển gần đây và việc sử dụng các hợp chất công cụ dược lý GPR84 để nghiên cứu vai trò và sinh học rộng hơn của nó."} +{"idx": 1870270, "en": "Norovirus-like particle (VLP) vaccine is promising against human norovirus infection. Unfortunately, genetic diversity of norovirus hindered the development of this vaccine. In this study, the immunogenicity of norovirus VLPs induced by the endemic GII.4 and the epidemic GII.17 genotypes, and the cross-reactivity between them as well as GI.1 and GII.3 VLPs were evaluated in mice by using serum IgG and histo-blood group antigen (HBGA) blocking antibodies as index. Results showed well immunogenicity of both GII.4 and GII.17 VLPs in mice. Serum IgG GMT (Geometric Mean Titer) were 3.63 (GII.4) and 3.88 (GII.17) respectively, and sustained to the 15th week. The HBGA blocking antibodies were 130 (GII.4) and 360 (GII.17) respectively at the end of the 4th week. Additionally, there was a dramatically statistical difference found in the cross-reactivity within genogroup (GII.3, GII.4 and GII.17) (p < .001), and also showed similar difference between genogroups (GI.1 vs. GII.3, GII.4 and GII.17) (p < .001). Summarized the pPICZa pichi pichia expression system showed a potential to be the alternative for expression of norovirus VLPs in secretion form, and the little cross-reactivity found between the endemic strain and the epidemic strain provides an evident for the consideration of selecting candidates of norovirus vaccine strains.", "gemini": "Vắc-xin hạt giống giống Norovirus (VLP) hứa hẹn chống lại nhiễm trùng norovirus ở người. Thật không may, sự đa dạng về mặt di truyền của norovirus đã cản trở sự phát triển của vắc-xin này. Trong nghiên cứu này, khả năng miễn dịch của VLP norovirus do kiểu gen GII.4 lưu hành và kiểu gen dịch GII.17 gây ra, và khả năng phản ứng chéo giữa chúng cũng như VLP GI.1 và GII.3 đã được đánh giá ở chuột bằng cách sử dụng IgG huyết thanh và kháng thể chặn kháng nguyên nhóm máu histo (HBGA) làm chỉ số. Kết quả cho thấy khả năng miễn dịch tốt của cả VLP GII.4 và GII.17 ở chuột. GMT IgG huyết thanh (tiêu đề trung bình hình học) lần lượt là 3,63 (GII.4) và 3,88 (GII.17) và duy trì đến tuần thứ 15. Kháng thể chặn HBGA lần lượt là 130 (GII.4) và 360 (GII.17) vào cuối tuần thứ 4. Ngoài ra, có sự khác biệt đáng kể về mặt thống kê về khả năng phản ứng chéo trong cùng nhóm gen (GII.3, GII.4 và GII.17) (p <0,001) và cũng cho thấy sự khác biệt tương tự giữa các nhóm gen (GI.1 so với GII.3, GII.4 và GII.17) (p <0,001). Tóm lại, hệ thống biểu hiện pPICZa pichi pichia cho thấy tiềm năng trở thành phương pháp thay thế để biểu hiện VLP norovirus ở dạng tiết và phản ứng chéo nhỏ được tìm thấy giữa chủng lưu hành và chủng dịch cung cấp bằng chứng rõ ràng cho việc xem xét lựa chọn các ứng cử viên chủng vắc-xin norovirus."} +{"idx": 1870271, "en": "Encapsulation properties of trypsin from tonggol tuna (Thunnus tonggol) spleen using different materials including alginate (AG), low and high molecular weight chitosan (LC and HC, respectively), and soy lecithin (SL) were studied. The highest encapsulation efficiency and greatest relative activity were found in AG/LC beads after simulated gastric phase (p < .05). AG/LC encapsulated trypsin was used in simulated in vitro gastrointestinal tract for hydrolysis of sodium caseinate, soy protein isolate and fish mince, in which all protein samples were hydrolyzed as indicated by the increased α-amino group content (p < .05). Higher degradation was attained when beads containing trypsin were added. When AG/LC beads packed in blister pack were stored for 8 weeks at refrigerated temperature, a 26% decrease in activity occurred. Therefore, encapsulated tonggol tuna spleen trypsin can be prepared using AG/LC to withstand structural breakdown in stomach, but be released as an active protease within intestinal tract. PRACTICAL APPLICATION: Spleen from tonggol tuna is a by-product, which can be used as a source of trypsin, a proteolytic enzyme. The trypsin that was encapsulated within alginate and low molecular weight chitosan beads was released in the intestinal phase and was retained proteolytic activity. Therefore, this encapsulated trypsin can be packaged in capsules and taken as a supplement to aid protein digestion in the gastrointestinal tract, especially for people that need such digestive aids.", "gemini": "Tính chất bao gói của trypsin từ lách cá ngừ tonggol (Thunnus tonggol) sử dụng các vật liệu khác nhau bao gồm alginate (AG), chitosan có trọng lượng phân tử thấp và cao (tương ứng là LC và HC) và lecithin đậu nành (SL) đã được nghiên cứu. Hiệu suất bao gói cao nhất và hoạt động tương đối lớn nhất được tìm thấy trong các hạt AG/LC sau pha dạ dày được mô phỏng (p <0,05). Trypsin được bao gói AG/LC được sử dụng trong đường tiêu hóa trong ống nghiệm được mô phỏng để thủy phân natri caseinat, protein đậu nành cô lập và cá xay, trong đó tất cả các mẫu protein đều được thủy phân như chỉ ra bằng hàm lượng nhóm α-amino tăng lên (p <0,05). Sự phân hủy cao hơn đạt được khi thêm các hạt chứa trypsin. Khi các hạt AG/LC được đóng gói trong vỉ được bảo quản ở nhiệt độ lạnh trong 8 tuần, hoạt động giảm 26%. Do đó, trypsin lách cá ngừ tonggol được bao gói có thể được chế biến bằng AG/LC để chịu được sự phân hủy về cấu trúc trong dạ dày, nhưng được giải phóng dưới dạng protease hoạt động trong đường ruột. ỨNG DỤNG THỰC TẾ: Lách cá ngừ tonggol là một sản phẩm phụ, có thể được sử dụng làm nguồn trypsin, một loại enzyme phân giải protein. Trypsin được bao gói trong các hạt alginate và chitosan có trọng lượng phân tử thấp được giải phóng trong giai đoạn ruột và giữ lại hoạt động phân giải protein. Do đó, trypsin được bao gói này có thể được đóng gói trong viên nang và dùng như một chất bổ sung để hỗ trợ tiêu hóa protein trong đường tiêu hóa, đặc biệt đối với những người cần các chất hỗ trợ tiêu hóa như vậy."} +{"idx": 1870272, "en": "OBJECTIVE This study aimed to compare the diagnostic performance of core-needle biopsy (CNB) to fine-needle aspiration (FNA) as a first-line diagnostic tool in initially detected thyroid nodules, according to ultrasound (US) patterns. MATERIALS AND METHODS This study included 778 consecutive nodules from 705 patients who underwent CNB from one institution and 627 nodules from 583 patients who underwent FNA from two institutions. Adjustments for significant differences in patients' characteristics were facilitated via propensity score matching. We compared the diagnostic performance of CNB and FNA for thyroid malignancy according to three diagnostic criteria for all nodules and the US patterns. RESULTS A 1:1 matching of 469 patients yielded no significant differences between CNB and FNA for any covariates. CNB showed a significantly higher sensitivity for malignancy than FNA with any criterion (criterion 1: category VI, criterion 2: category V and VI, criterion 3: category IV, V and VI) in overall and high suspicion nodules (90.1-99.5% vs 69.7%-88.3%, all P-values < 0.001) and low/intermediate suspicion nodules, except criterion 1 (61.9%-100% vs 36.4%-45.5%, all P ≤ .016). In ROC curve analysis, the areas under the ROC curve of CNB were significantly higher than those for FNA with any criterion in overall and high suspicion nodules (P < .001) and in low/intermediate suspicion nodules, except criterion 1 (P ≤ .008). CNB had a slightly higher minor complication rate than FNA (0.7% vs 0%, P ≥ .069). CONCLUSION Our study suggests that CNB has a complementary role as an alternative first-line diagnostic tool to FNA for the initial diagnosis of thyroid nodules when performed by an experienced operator.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm so sánh hiệu suất chẩn đoán của sinh thiết lõi kim (CNB) với chọc hút kim nhỏ (FNA) như một công cụ chẩn đoán tuyến đầu trong các nốt sần tuyến giáp được phát hiện ban đầu, theo các mô hình siêu âm (US). VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu này bao gồm 778 nốt sần liên tiếp từ 705 bệnh nhân đã trải qua CNB từ một cơ sở và 627 nốt sần từ 583 bệnh nhân đã trải qua FNA từ hai cơ sở. Việc điều chỉnh các khác biệt đáng kể về đặc điểm của bệnh nhân được thực hiện thông qua ghép điểm khuynh hướng. Chúng tôi đã so sánh hiệu suất chẩn đoán của CNB và FNA đối với ác tính tuyến giáp theo ba tiêu chuẩn chẩn đoán đối với tất cả các nốt sần và các mô hình siêu âm. KẾT QUẢ Việc ghép 1:1 của 469 bệnh nhân không cho thấy sự khác biệt đáng kể nào giữa CNB và FNA đối với bất kỳ biến số nào. CNB cho thấy độ nhạy đối với ác tính cao hơn đáng kể so với FNA với bất kỳ tiêu chuẩn nào (tiêu chuẩn 1: loại VI, tiêu chuẩn 2: loại V và VI, tiêu chuẩn 3: loại IV, V và VI) ở các nốt sần nghi ngờ chung và cao (90,1-99,5% so với 69,7% -88,3%, tất cả các giá trị P <0,001) và các nốt sần nghi ngờ thấp/trung bình, ngoại trừ tiêu chuẩn 1 (61,9% -100% so với 36,4% -45,5%, tất cả các giá trị P ≤ 0,016). Trong phân tích đường cong ROC, diện tích dưới đường cong ROC của CNB cao hơn đáng kể so với FNA với bất kỳ tiêu chuẩn nào ở các nốt sần nghi ngờ chung và cao (P <0,001) và ở các nốt sần nghi ngờ thấp/trung bình, ngoại trừ tiêu chuẩn 1 (P ≤ 0,008). CNB có tỷ lệ biến chứng nhỏ cao hơn một chút so với FNA (0,7% so với 0%, P ≥ 0,069). KẾT LUẬN Nghiên cứu của chúng tôi cho thấy CNB có vai trò bổ sung như một công cụ chẩn đoán tuyến đầu thay thế cho FNA để chẩn đoán ban đầu các nốt sần tuyến giáp khi được thực hiện bởi một bác sĩ phẫu thuật có kinh nghiệm."} +{"idx": 1870273, "en": "METHOD Two hundred Hispanic emerging adults from Arizona (n = 99) and Florida (n = 101) completed a cross-sectional survey, and data were analyzed using hierarchical multiple regression and moderation analyses. RESULTS Higher social media discrimination was associated with higher symptoms of depression and generalized anxiety. Moderation analyses indicated that higher social media discrimination was only associated with symptoms of depression and generalized anxiety among men, but not women. CONCLUSION This is likely the first study on social media discrimination and mental health among emerging adults; thus, expanding this emerging field of research to a distinct developmental period.", "gemini": "PHƯƠNG PHÁP Hai trăm thanh thiếu niên gốc Tây Ban Nha mới nổi từ Arizona (n = 99) và Florida (n = 101) đã hoàn thành một cuộc khảo sát cắt ngang và dữ liệu được phân tích bằng cách hồi quy bội và phân tích điều độ phân cấp. KẾT QUẢ Phân biệt trên mạng xã hội cao hơn có liên quan đến các triệu chứng trầm cảm và lo âu lan tỏa cao hơn. Phân tích điều độ chỉ ra rằng sự phân biệt trên mạng xã hội cao hơn chỉ liên quan đến các triệu chứng trầm cảm và lo âu lan tỏa ở nam giới, nhưng không phải ở nữ giới. KẾT LUẬN Đây có thể là nghiên cứu đầu tiên về sự phân biệt đối xử trên mạng xã hội và sức khỏe tâm thần ở những người trẻ mới lớn; do đó, mở rộng lĩnh vực nghiên cứu mới nổi này sang một giai đoạn phát triển khác biệt."} +{"idx": 1870274, "en": "BACKGROUND Ginkgotoxin including 4'-O-methylpyridoxine (MPN) and MPN-5'-glucoside (MPNG) is responsible for Ginkgo seed food poisoning. The purpose of the work reported was to prepare detoxified Ginkgo seed powder and at the same time to retain the nutritional and functional components of Ginkgo seed powder to the maximum extent. RESULTS Resin adsorption technology was firstly employed to remove ginkgotoxin from water extract of Ginkgo seed powder. Under optimal conditions, the adsorption efficiency of the optimal resin for MPN could reach 100%, and that for MPNG could only reach 85.4 ± 0.93%. Resin adsorption alone could not effectively remove MPN and MPNG simultaneously. Endogenous enzymatic hydrolysis was next attempted to transform MPNG to MPN. MPNG could be completely hydrolyzed to MPN by endogenous enzyme(s) at 40 °C and pH 5.0 in 180 min. Ginkgotoxin only in the form of MPN in the enzymatic hydrolysate was then adsorbed with resin and the conditions were statistically optimized. The adsorption efficiency of MPN reached 98.89 ± 0.99% under the optimized conditions. CONCLUSIONS Removal of ginkgotoxin by combining endogenous enzymatic hydrolysis with resin adsorption could preserve the main nutritional and functional components of Ginkgo seed powder to the most extent, and did not change its main characteristics. The ginkgotoxin removal method developed in this work is a relatively simple and efficient approach. © 2020 Society of Chemical Industry.", "gemini": "BỐI CẢNH Ginkgotoxin bao gồm 4'-O-methylpyridoxine (MPN) và MPN-5'-glucoside (MPNG) là nguyên nhân gây ngộ độc thực phẩm từ hạt bạch quả. Mục đích của công trình được báo cáo là chế biến bột hạt bạch quả đã khử độc và đồng thời giữ lại các thành phần dinh dưỡng và chức năng của bột hạt bạch quả ở mức tối đa. KẾT QUẢ Công nghệ hấp phụ nhựa đầu tiên được sử dụng để loại bỏ ginkgotoxin khỏi chiết xuất nước của bột hạt bạch quả. Trong điều kiện tối ưu, hiệu suất hấp phụ của nhựa tối ưu đối với MPN có thể đạt 100% và đối với MPNG chỉ đạt 85,4 ± 0,93%. Chỉ riêng quá trình hấp phụ nhựa không thể loại bỏ hiệu quả MPN và MPNG cùng lúc. Tiếp theo, người ta đã thử thủy phân bằng enzym nội sinh để chuyển đổi MPNG thành MPN. MPNG có thể được thủy phân hoàn toàn thành MPN bằng enzym (các) nội sinh ở 40 ° C và pH 5,0 trong 180 phút. Ginkgotoxin chỉ ở dạng MPN trong dịch thủy phân bằng enzym sau đó được hấp phụ bằng nhựa và các điều kiện được tối ưu hóa về mặt thống kê. Hiệu suất hấp phụ của MPN đạt 98,89 ± 0,99% trong điều kiện tối ưu. KẾT LUẬN Loại bỏ ginkgotoxin bằng cách kết hợp thủy phân bằng enzym nội sinh với hấp phụ nhựa có thể bảo toàn các thành phần dinh dưỡng và chức năng chính của bột hạt bạch quả ở mức độ cao nhất và không làm thay đổi các đặc tính chính của bột hạt bạch quả. Phương pháp loại bỏ ginkgotoxin được phát triển trong công trình này là một phương pháp tiếp cận tương đối đơn giản và hiệu quả. © 2020 Hội Công nghiệp Hóa học."} +{"idx": 1870275, "en": "A formal reductive [3+2] annulation of 4,4,4-trifluorocrotonate and α-iminoketones was developed under Brønsted base catalysis. A single phosphazene base efficiently catalyzes the one-pot tandem reaction involving two mechanistically different elementary processes, namely the chemoselective reduction of an imine moiety of α-iminoketones with thiols as the reductant and the subsequent intermolecular Michael addition of an enolate of α-aminoketones concomitant with lactam formation. This operationally simple method provides β-trifluoromethyl-substituted γ-lactams with a tetrasubstituted carbon as a single diastereomer.", "gemini": "Một phản ứng vòng hóa khử dạng [3 + 2] chính thức của 4,4,4-trifluorocrotonat và α-iminoketon đã được phát triển dưới sự xúc tác của bazơ Brønsted. Một bazơ phosphazene duy nhất xúc tác hiệu quả phản ứng song song một nồi liên quan đến hai quá trình cơ bản khác nhau về mặt cơ chế, cụ thể là quá trình khử hóa học chọn lọc của một nhóm imin của α-iminoketon với các thiol là chất khử và quá trình cộng Michael liên phân tử tiếp theo của một enolat của α-iminoketon cùng với sự hình thành lactam. Phương pháp đơn giản về mặt vận hành này cung cấp các γ-lactam được thay thế β-trifluoromethyl với cacbon được thay thế bốn lần làm một đồng phân lập thể duy nhất."} +{"idx": 1870276, "en": "We used behavioral testing and morphological methods to detail the progression of basal ganglia neuron type-specific pathology and the deficits stemming from them in male heterozygous Q175 mice, compared to age-matched WT males. A rotarod deficit was not present in Q175 mice until 18 months, but increased open field turn rate (reflecting hyperkinesia) and open field anxiety were evident at 6 months. No loss of striatal neurons was seen out to 18 months, but ENK+ and DARPP32+ striatal perikarya were fewer by 6 months, due to diminished expression, with further decline by 18 months. No reduction in SP+ striatal perikarya or striatal interneurons was seen in Q175 mice at 18 months, but cholinergic interneurons showed dendrite attenuation by 6 months. Despite reduced ENK expression in indirect pathway striatal perikarya, ENK-immunostained terminals in globus pallidus externus (GPe) were more abundant at 6 months and remained so out to 18 months. Similarly, SP-immunostained terminals from striatal direct pathway neurons were more abundant in globus pallidus internus and substantia nigra at 6 months and remained so at 18 months. FoxP2+ arkypallidal GPe neurons and subthalamic nucleus neurons were lost by 18 months but not prototypical PARV+ GPe neurons or dopaminergic nigral neurons. Our results show that striatal projection neuron abnormalities and behavioral abnormalities reflecting them develop between 2 and 6 months of age in Q175 male heterozygotes, indicating early effects of the HD mutation. The striatal pathologies resemble those in human HD, but are less severe at 18 months than even in premanifest HD.", "gemini": "Chúng tôi đã sử dụng phương pháp kiểm tra hành vi và phương pháp hình thái để mô tả chi tiết quá trình tiến triển của bệnh lý đặc hiệu theo loại nơ-ron hạch nền và các khiếm khuyết bắt nguồn từ chúng ở chuột Q175 dị hợp tử đực, so với chuột đực WT cùng độ tuổi. Chuột Q175 không gặp tình trạng khiếm khuyết trên rotarod cho đến 18 tháng, nhưng tốc độ quay ở bãi mở tăng lên (phản ánh tình trạng tăng động) và lo âu ở bãi mở đã biểu hiện rõ ở 6 tháng. Không thấy mất tế bào thần kinh ở vùng vân cho đến 18 tháng, nhưng các nhân tế bào thần kinh ở vùng vân ENK+ và DARPP32+ ít hơn vào 6 tháng, do biểu hiện giảm, với sự suy giảm thêm vào 18 tháng. Không thấy giảm các nhân tế bào thần kinh ở vùng vân SP+ hoặc các tế bào thần kinh đệm ở vùng vân ở chuột Q175 ở 18 tháng, nhưng các tế bào thần kinh đệm cholinergic cho thấy sự suy giảm các nhánh cây dendrite ở 6 tháng. Mặc dù biểu hiện ENK giảm ở các nhân tế bào thần kinh ở vùng vân trên đường dẫn gián tiếp, nhưng các đầu mút nhuộm miễn dịch ENK trong phần ngoài của cầu nhạt (GPe) lại nhiều hơn vào 6 tháng và vẫn như vậy cho đến 18 tháng. Tương tự, các đầu mút nhuộm miễn dịch SP từ các tế bào thần kinh đường dẫn trực tiếp ở vùng vân nhiều hơn ở phần trong của cầu nhạt và chất đen ở 6 tháng và vẫn như vậy ở 18 tháng. Các tế bào thần kinh GPe arkypallidal FoxP2+ và các tế bào thần kinh nhân dưới đồi bị mất vào 18 tháng nhưng không phải các tế bào thần kinh GPe PARV+ nguyên mẫu hoặc các tế bào thần kinh dopaminergic ở chất đen. Kết quả của chúng tôi cho thấy các bất thường ở tế bào thần kinh chiếu ở vùng vân và các bất thường về hành vi phản ánh chúng phát triển trong khoảng từ 2 đến 6 tháng tuổi ở chuột dị hợp tử đực Q175, cho thấy những ảnh hưởng sớm của đột biến HD. Các bệnh lý ở vùng vân giống với những bệnh lý ở HD ở người, nhưng ít nghiêm trọng hơn vào 18 tháng so với HD tiền triệu chứng."} +{"idx": 1870277, "en": "OBJECTIVE Despite nonsuicidal self-injury (NSSI) being a prevalent and problematic behavior, only approximately half of those who engage in NSSI disclose their behavior. Yet, limited research has explored the choice to disclose. This study sought to identify if NSSI characteristics, emotional distress, and perceived interpersonal obstacles discriminated between NSSI disclosure status. Exploratory aims also investigated reasons for one's disclosure decision and disclosure contextual factors. METHOD Participants included 977 undergraduate students (83% female) with a lifetime history of NSSI. RESULTS Greater NSSI intrapersonal functions, suicide risk, and significant other support, and lower depression symptoms were associated with NSSI disclosure. Exploratory results highlight perceptions of one's NSSI severity and desire to receive support in disclosure choice; intrapersonal functions and peer support were associated with the timing of disclosure. CONCLUSIONS Findings underscore the potential importance of individual attitudes toward NSSI, in addition to traditionally measured risk factors, as potential drivers in NSSI disclosure.", "gemini": "MỤC TIÊU Mặc dù tự gây hại không tự sát (NSSI) là một hành vi phổ biến và có vấn đề, nhưng chỉ có khoảng một nửa số người tham gia NSSI tiết lộ hành vi của họ. Tuy nhiên, nghiên cứu hạn chế đã khám phá sự lựa chọn tiết lộ. Nghiên cứu này tìm cách xác định xem các đặc điểm của NSSI, sự đau khổ về mặt cảm xúc và những trở ngại giữa các cá nhân được nhận thức có phân biệt giữa trạng thái tiết lộ NSSI hay không. Các mục đích thăm dò cũng điều tra lý do cho quyết định tiết lộ và các yếu tố bối cảnh tiết lộ. PHƯƠNG PHÁP Những người tham gia bao gồm 977 sinh viên đại học (83% là nữ) có tiền sử NSSI. KẾT QUẢ Các chức năng nội tâm của NSSI lớn hơn, nguy cơ tự tử và sự hỗ trợ đáng kể của người khác, và các triệu chứng trầm cảm thấp hơn có liên quan đến việc tiết lộ NSSI. Kết quả thăm dò nêu bật nhận thức về mức độ nghiêm trọng của NSSI và mong muốn nhận được sự hỗ trợ trong lựa chọn tiết lộ; các chức năng nội tâm và sự hỗ trợ của bạn bè có liên quan đến thời điểm tiết lộ. KẾT LUẬN Các phát hiện nhấn mạnh tầm quan trọng tiềm tàng của các thái độ cá nhân đối với NSSI, ngoài các yếu tố rủi ro được đo lường theo truyền thống, là động lực tiềm ẩn trong việc tiết lộ NSSI."} +{"idx": 1870278, "en": "BACKGROUND Behçet disease (BD) is an immune-mediated vasculitis-like syndrome characterized by recurrent aphthous lesions and various systemic manifestations. Inflammatory markers may be useful to assess disease severity. The Systemic Immune-Inflammation Index (SII) (neutrophils × platelets/lymphocytes) has been widely used in oncology since 2014, with promising results. AIM To assess the efficiency of the SII in determining activity of BD. METHODS This retrospective cohort study was conducted on patients with BD who were admitted to the outpatient clinic of the Department of Dermatology and Venereology, Ufuk University Hospital, between 1 January 2010 and 31 December 2019. Patients were divided into two groups based on their disease status upon admission: (i) active BD (n = 103), and (ii) inactive BD (n = 63). Clinical characteristics, demographic features, type of medications, full blood count parameters, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), ferritin and SII were compared between the groups. Furthermore, receiver operating characteristic curve analysis was performed to assess the performance of the SII in determining disease severity upon admission to hospital. RESULTS Higher numbers of white blood cells, platelets and neutrophils, greater red cell distribution width, higher levels of ESR, CRP and ferritin, and higher SII were observed in the active disease group (P < 0.001). The cutoff value of 552 × 103 /mm3 was found to have 81% sensitivity and 82% specificity. CONCLUSION The SII may be used as an additional indicator for the assessment of BD status and physicians should be cautious in patients with SII levels of > 552 × 103 /mm3 ) at the initial evaluation of the patients.", "gemini": "BỐI CẢNH Bệnh Behçet (BD) là một hội chứng giống viêm mạch do miễn dịch đặc trưng bởi các tổn thương loét miệng tái phát và nhiều biểu hiện toàn thân khác nhau. Các dấu hiệu viêm có thể hữu ích để đánh giá mức độ nghiêm trọng của bệnh. Chỉ số viêm miễn dịch toàn thân (SII) (bạch cầu trung tính × tiểu cầu/lympho bào) đã được sử dụng rộng rãi trong ung thư từ năm 2014, với kết quả đầy hứa hẹn. MỤC ĐÍCH Đánh giá hiệu quả của SII trong việc xác định hoạt động của BD. PHƯƠNG PHÁP Nghiên cứu đoàn hệ hồi cứu này được tiến hành trên những bệnh nhân mắc BD được nhập viện tại phòng khám ngoại trú của Khoa Da liễu và Bệnh xã hội, Bệnh viện Đại học Ufuk, từ ngày 1 tháng 1 năm 2010 đến ngày 31 tháng 12 năm 2019. Bệnh nhân được chia thành hai nhóm dựa trên tình trạng bệnh khi nhập viện: (i) BD hoạt động (n = 103) và (ii) BD không hoạt động (n = 63). Các đặc điểm lâm sàng, đặc điểm nhân khẩu học, loại thuốc, thông số công thức máu toàn bộ, tốc độ lắng máu hồng cầu (ESR), protein phản ứng C (CRP), ferritin và SII được so sánh giữa các nhóm. Hơn nữa, phân tích đường cong đặc trưng hoạt động của người nhận đã được thực hiện để đánh giá hiệu suất của SII trong việc xác định mức độ nghiêm trọng của bệnh khi nhập viện. KẾT QUẢ Số lượng bạch cầu, tiểu cầu và bạch cầu trung tính cao hơn, độ rộng phân bố hồng cầu lớn hơn, mức ESR, CRP và ferritin cao hơn và SII cao hơn được quan sát thấy ở nhóm bệnh hoạt động (P < 0,001). Giá trị ngưỡng là 552 × 103 /mm3 được phát hiện có độ nhạy 81% và độ đặc hiệu 82%. KẾT LUẬN SII có thể được sử dụng như một chỉ báo bổ sung để đánh giá tình trạng BD và các bác sĩ nên thận trọng ở những bệnh nhân có mức SII > 552 × 103 /mm3) khi đánh giá ban đầu bệnh nhân."} +{"idx": 1870279, "en": "AIM The aim was to investigate the use of paper-based and electronic prescribing and resuscitation aids in paediatric emergency care from a departmental and individual physician perspective. METHODS A two-stage web-based self-report questionnaire was performed. In stage (i), a lead investigator at PERUKI sites completed a department-level survey; in stage (ii), individual physicians recorded their personal practice. RESULTS The site survey was completed by 46/54 (85%) of PERUKI sites. 198 physicians completed the individual physicians' survey. Individual physicians selected the use of formulary apps for checking of medication dosages nearly as often as hardcopy formularies. The APLS WETFLAG calculation and hardcopy aids were widely accepted in both surveys. A third of sites accepted and half of the individual physicians selected resuscitation apps on the personal mobile device as paediatric resuscitation aids. CONCLUSION Our survey shows a high penetrance of the British National Formulary app, a success of NHS digital policy and strategy. Despite potential advantages, many physicians in our survey do not use resuscitation apps. Reluctance to engage with apps is likely to be multifactorial and includes human factors. These obstacles need to be overcome to create a digital healthcare culture.", "gemini": "MỤC ĐÍCH Mục đích là để điều tra việc sử dụng các dụng cụ hỗ trợ hồi sức và kê đơn điện tử và giấy trong chăm sóc cấp cứu nhi khoa từ góc độ của khoa và bác sĩ cá nhân. PHƯƠNG PHÁP Một bảng câu hỏi tự báo cáo dựa trên web gồm hai giai đoạn đã được thực hiện. Ở giai đoạn (i), một điều tra viên chính tại các địa điểm PERUKI đã hoàn thành một cuộc khảo sát cấp khoa; ở giai đoạn (ii), các bác sĩ cá nhân ghi lại thực hành cá nhân của họ. KẾT QUẢ Cuộc khảo sát tại địa điểm đã được 46/54 (85%) địa điểm PERUKI hoàn thành. 198 bác sĩ đã hoàn thành cuộc khảo sát của các bác sĩ cá nhân. Các bác sĩ cá nhân đã chọn sử dụng các ứng dụng công thức để kiểm tra liều lượng thuốc gần như thường xuyên như các công thức cứng. Việc tính toán WETFLAG của APLS và các dụng cụ cứng đã được chấp nhận rộng rãi trong cả hai cuộc khảo sát. Một phần ba các địa điểm đã chấp nhận và một nửa số bác sĩ cá nhân đã chọn các ứng dụng hồi sức trên thiết bị di động cá nhân làm dụng cụ hồi sức nhi khoa. KẾT LUẬN Cuộc khảo sát của chúng tôi cho thấy ứng dụng British National Formulary có tỷ lệ thâm nhập cao, đây là thành công của chiến lược và chính sách kỹ thuật số của NHS. Mặc dù có nhiều lợi thế tiềm ẩn, nhiều bác sĩ trong cuộc khảo sát của chúng tôi không sử dụng các ứng dụng hồi sức. Sự miễn cưỡng tham gia với các ứng dụng có thể là do nhiều yếu tố và bao gồm các yếu tố của con người. Những trở ngại này cần phải được khắc phục để tạo ra một nền văn hóa chăm sóc sức khỏe kỹ thuật số."} +{"idx": 1870280, "en": "Hair loss and thinning are possible complications in those undergoing endocrine therapies with aromatase inhibitors. Alopecia in pediatric patients undergoing endocrine therapy has not been previously reported. We describe two adolescents, 14 and 16 years of age, who developed androgenetic alopecia following treatment with anastrozole for idiopathic short stature. Accordingly, the possible adverse event of alopecia should be considered in the pediatric population undergoing treatment with aromatase inhibitors.", "gemini": "Rụng tóc và tóc mỏng là những biến chứng có thể xảy ra ở những người đang trải qua liệu pháp nội tiết bằng thuốc ức chế aromatase. Chứng rụng tóc ở bệnh nhi đang trải qua liệu pháp nội tiết chưa từng được báo cáo trước đây. Chúng tôi mô tả hai thanh thiếu niên, 14 và 16 tuổi, bị rụng tóc theo kiểu androgen sau khi điều trị bằng anastrozole cho tình trạng lùn vô căn. Theo đó, nên cân nhắc tác dụng phụ có thể xảy ra là rụng tóc ở trẻ em đang được điều trị bằng thuốc ức chế aromatase."} +{"idx": 1870281, "en": "Studies of non-nutritive sweeteners (NNS) in diabetes models have been limited to their pure forms or NNS-sweetened products. Hence, we conducted a comparative study on the effects of commercial table-top NNS on diabetes-related parameters in non-diabetic rats. Normal animals were fed for 5 weeks with aqueous solutions of aspartame-, sucralose-, stevia-, sodium cyclamate- and saccharin-based commercial NNS at concentrations equivalent to the sweetness of 10% sucrose solution and thereafter food intake, blood glucose, lipid profile, and biochemical parameters were measured. Aspartame adversely affected blood cholesterols, while cyclamate increased food intake and weight gain. Stevia reduced weight gain and exhibited insulinotropic effects. These data in normal rats hypothetically suggest that stevia-based NNS may help in glycemic control and body weight management, while cyclamate- and aspartame-based NNS may increase body weight and risk of cardiovascular diseases. Further clinical studies are, however, required to confirm the results of this study. PRACTICAL APPLICATIONS: The use of NNS is becoming more popular, especially for individuals with diabetes. However, while there are several commercial table-top NNS available in the market, little is known about how they affect most diabetes-related parameters of consumers, as most of the previous studies on NNS have been limited to their pure forms or NNS-sweetened products. Therefore, we comparatively studied the effects of some commercially available table-top forms of the different NNS (aspartame, sucralose, cyclamate, saccharin, and stevia) on diabetes-related parameters in normal rats. These findings in normal rats suggested that some commercially available NNSs like stevia-based NNS may be suitable for glycemic control and body weight management, while cyclamate- and aspartame-based NNS may increase body weight and risk of cardiovascular diseases. However, these finding in normal rats is subject to additional corroborative clinical studies.", "gemini": "Các nghiên cứu về chất tạo ngọt không dinh dưỡng (NNS) trong các mô hình bệnh tiểu đường chỉ giới hạn ở dạng tinh khiết hoặc các sản phẩm có thêm NNS. Do đó, chúng tôi đã tiến hành một nghiên cứu so sánh về tác động của NNS thương mại trên bàn đối với các thông số liên quan đến bệnh tiểu đường ở chuột không mắc bệnh tiểu đường. Động vật bình thường được cho ăn trong 5 tuần bằng dung dịch nước của NNS thương mại có thành phần aspartame, sucralose, stevia, natri cyclamate và saccharin với nồng độ tương đương với độ ngọt của dung dịch sucrose 10% và sau đó lượng thức ăn nạp vào, lượng đường trong máu, hồ sơ lipid và các thông số sinh hóa được đo. Aspartame ảnh hưởng xấu đến cholesterol trong máu, trong khi cyclamate làm tăng lượng thức ăn nạp vào và tăng cân. Stevia làm giảm tăng cân và biểu hiện tác dụng kích thích insulin. Những dữ liệu này ở chuột bình thường về mặt giả thuyết cho thấy rằng NNS có thành phần stevia có thể giúp kiểm soát đường huyết và quản lý cân nặng, trong khi NNS có thành phần cyclamate và aspartame có thể làm tăng cân và nguy cơ mắc các bệnh tim mạch. Tuy nhiên, cần có thêm các nghiên cứu lâm sàng để xác nhận kết quả của nghiên cứu này. CÁC ỨNG DỤNG THỰC TẾ: Việc sử dụng NNS ngày càng trở nên phổ biến, đặc biệt là đối với những người mắc bệnh tiểu đường. Tuy nhiên, trong khi có một số loại NNS thương mại trên bàn có sẵn trên thị trường, người ta vẫn biết rất ít về cách chúng ảnh hưởng đến hầu hết các thông số liên quan đến bệnh tiểu đường của người tiêu dùng, vì hầu hết các nghiên cứu trước đây về NNS đều chỉ giới hạn ở dạng tinh khiết hoặc các sản phẩm có thêm NNS. Do đó, chúng tôi đã so sánh nghiên cứu tác động của một số dạng NNS khác nhau có sẵn trên thị trường (aspartame, sucralose, cyclamate, saccharin và stevia) đối với các thông số liên quan đến bệnh tiểu đường ở chuột bình thường. Những phát hiện này ở chuột bình thường cho thấy một số NNS có sẵn trên thị trường như NNS có thành phần stevia có thể phù hợp để kiểm soát đường huyết và quản lý cân nặng, trong khi NNS có thành phần cyclamate và aspartame có thể làm tăng cân và nguy cơ mắc các bệnh tim mạch. Tuy nhiên, những phát hiện này ở chuột bình thường vẫn phải tùy thuộc vào các nghiên cứu lâm sàng bổ sung."} +{"idx": 1870282, "en": "BACKGROUND AND OBJECTIVES Picosecond lasers in dermatology were originally focused on optimizing the removal of unwanted tattoos. Subsequent advances in this technology have expanded its clinical indications to include treatment of benign pigmented lesions, photodamage, melasma, and scar revision. In this retrospective review, we evaluate a novel 730 nm picosecond titanium sapphire laser in treating benign pigmented lesions. STUDY DESIGN/MATERIALS AND METHODS This is a retrospective review of all patients who presented to our institution between December 2019 and March 2020 for treatment of their benign pigmented lesions with a 730 nm picosecond titanium sapphire laser. All Fitzpatrick skin types (I-VI) were included. Absolute and relative evaluations were conducted by two blinded board-certified dermatologists using high-resolution photographic images. RESULTS Twenty-two of 64 patients satisfied inclusion and exclusion criteria. Patients received 1.1 ± 0.3 treatment sessions. The absolute average pigmentation score prior to treatment was 2.04 ± 0.7 versus 1.39 ± 0.6 after treatment (P < 0.05). Improvement in pigmentation was observed in 86% of the patients, while 3% had no improvement and 11% had worsening of pigmentation. No other adverse events were observed. Downtime consisted of 1-2 days of mild edema and erythema followed by 3-5 days of mild pigment darkening and superficial crust. CONCLUSION The novel 730 nm picosecond titanium sapphire laser is a safe and effective treatment for benign pigmented lesions. Future prospective randomized control studies would be beneficial to further clarify its role in the treatment of benign pigmentation. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.", "gemini": "BỐI CẢNH VÀ MỤC TIÊU Ban đầu, laser picosecond trong da liễu tập trung vào việc tối ưu hóa việc xóa các hình xăm không mong muốn. Những tiến bộ tiếp theo trong công nghệ này đã mở rộng các chỉ định lâm sàng của nó bao gồm điều trị các tổn thương sắc tố lành tính, tổn thương do ánh sáng, nám da và chỉnh sửa sẹo. Trong bài đánh giá hồi cứu này, chúng tôi đánh giá một loại laser sapphire titan picosecond 730 nm mới trong điều trị các tổn thương sắc tố lành tính. THIẾT KẾ NGHIÊN CỨU/VẬT LIỆU VÀ PHƯƠNG PHÁP Đây là một đánh giá hồi cứu tất cả các bệnh nhân đến cơ sở của chúng tôi trong khoảng thời gian từ tháng 12 năm 2019 đến tháng 3 năm 2020 để điều trị các tổn thương sắc tố lành tính của họ bằng laser sapphire titan picosecond 730 nm. Tất cả các loại da Fitzpatrick (I-VI) đều được đưa vào. Hai bác sĩ da liễu được chứng nhận là chuyên gia đã tiến hành các đánh giá tuyệt đối và tương đối bằng cách sử dụng hình ảnh chụp độ phân giải cao. KẾT QUẢ Hai mươi hai trong số 64 bệnh nhân đáp ứng các tiêu chuẩn loại trừ và bao gồm. Bệnh nhân được điều trị trung bình 1,1 ± 0,3 lần. Điểm sắc tố trung bình tuyệt đối trước khi điều trị là 2,04 ± 0,7 so với 1,39 ± 0,6 sau khi điều trị (P < 0,05). Sự cải thiện sắc tố được quan sát thấy ở 86% bệnh nhân, trong khi 3% không cải thiện và 11% bị sắc tố nặng hơn. Không có tác dụng phụ nào khác được quan sát thấy. Thời gian ngừng hoạt động bao gồm 1-2 ngày phù nề nhẹ và ban đỏ, sau đó là 3-5 ngày sắc tố sẫm màu nhẹ và lớp vỏ nông. KẾT LUẬN Laser sapphire titan picosecond 730 nm mới là phương pháp điều trị an toàn và hiệu quả đối với các tổn thương sắc tố lành tính. Các nghiên cứu kiểm soát ngẫu nhiên có triển vọng trong tương lai sẽ có lợi để làm rõ hơn vai trò của nó trong điều trị tăng sắc tố lành tính. Lasers Surg. Med. © 2020 Wiley Periodicals LLC."} +{"idx": 1870283, "en": "The introduction of personalized medicine (PM) has been a milestone in the history of medical therapy, because it has revolutionized the previous approach of treating the disease with that of treating the patient. It is known today that diseases can occur in different genetic variants, making specific treatments of proven efficacy necessary for a given endotype. Allergic diseases are particularly suitable for PM, because they meet the therapeutic success requirements, including a known molecular mechanism of the disease, a diagnostic tool for such disease, and a treatment blocking the mechanism. The stakes of PM in allergic patients are molecular diagnostics, to detect specific IgE to single-allergen molecules and to distinguish the causative molecules from those merely cross-reactive, pursuit of patient's treatable traits addressing genetic, phenotypic, and psychosocial features, and omics, such as proteomics, epi-genomics, metabolomics, and breathomics, to forecast patient's responsiveness to therapies, to detect biomarker and mediators, and to verify the disease control. This new approach has already improved the precision of allergy diagnosis and is likely to significantly increase, through the higher performance achieved with the personalized treatment, the effectiveness of allergen immunotherapy by enhancing its already known and unique characteristics of treatment that acts on the causes.", "gemini": "Việc đưa ra y học cá nhân hóa (PM) là một cột mốc trong lịch sử của liệu pháp y tế, bởi vì nó đã cách mạng hóa phương pháp điều trị bệnh trước đây bằng phương pháp điều trị bệnh nhân. Ngày nay người ta biết rằng các bệnh có thể xảy ra ở các biến thể di truyền khác nhau, do đó cần có các phương pháp điều trị cụ thể có hiệu quả đã được chứng minh đối với một kiểu hình nhất định. Các bệnh dị ứng đặc biệt phù hợp với PM, vì chúng đáp ứng các yêu cầu về thành công của liệu pháp, bao gồm cơ chế phân tử đã biết của bệnh, một công cụ chẩn đoán cho bệnh đó và một phương pháp điều trị ngăn chặn cơ chế này. Các mục tiêu của PM ở bệnh nhân dị ứng là chẩn đoán phân tử, để phát hiện IgE đặc hiệu với các phân tử dị nguyên đơn và phân biệt các phân tử gây bệnh với các phân tử chỉ phản ứng chéo, theo đuổi các đặc điểm có thể điều trị của bệnh nhân liên quan đến các đặc điểm di truyền, kiểu hình và tâm lý xã hội và các yếu tố như proteomics, epi-genomics, metabolomics và breathomics, để dự báo khả năng đáp ứng của bệnh nhân với các liệu pháp, phát hiện ra các dấu ấn sinh học và chất trung gian và xác minh kiểm soát bệnh. Phương pháp tiếp cận mới này đã cải thiện độ chính xác của chẩn đoán dị ứng và có khả năng làm tăng đáng kể, thông qua hiệu suất cao hơn đạt được với phương pháp điều trị cá nhân hóa, hiệu quả của liệu pháp miễn dịch dị nguyên bằng cách tăng cường các đặc điểm điều trị đã biết và độc đáo của nó, phương pháp điều trị tác động vào nguyên nhân."} +{"idx": 1870284, "en": "Exercise training is a well-known lifestyle to maintain good health. The present study was conducted to explore the effect of regular exercise training (for 15 min) on biochemical, physiological, and histological changes in fried oil intake (5 g/kg body weight, for 5 weeks) with or without raw oil supplementation. Liver disease and heart muscle injury were accounted for by significant (p < .05) increase in liver and heart biomarker enzymes in serum. Creatinine and urea level were also significantly increased in the fried oil-fed group as a sign of kidney injury. But all the biological markers including triglycerides, low-density lipoprotein cholesterol (LDL-C) was decreased significantly (p < .05) in the exercise-trained rat group. These metabolic changes were substantiated by the histological study of respective organs. Therefore, people should avoid repeatedly deep-fried oil consumption rather than fresh ones though regular exercise training has been found to resolve these metabolic abnormalities. PRACTICAL APPLICATIONS: Exercise training is a common practice to reduce the onset of some metabolic abnormalities for example cardiovascular disease, non-alcoholic fatty liver disease, chronic kidney disease, and also prevents various cell and tissue damages. Repeated frying of vegetable oil not only reduces its nutritional value but also produce free fatty acids and other toxic compounds. The present study revealed that repeatedly heated vegetable oil consumption causes injuries in the heart, liver, kidney, and small intestine by the oxidative products. As a result, the level of biomarkers of these responsive organs was found to be elevated. But regular exercise training ameliorated these detrimental effects. So, in terms of public health concern, regular exercise is one of the best ways to keep the body fit especially those who are consuming deep-fried oil.", "gemini": "Tập thể dục là một lối sống nổi tiếng để duy trì sức khỏe tốt. Nghiên cứu hiện tại được tiến hành để khám phá tác dụng của việc tập thể dục thường xuyên (trong 15 phút) đối với các thay đổi về sinh hóa, sinh lý và mô học khi ăn dầu chiên (5 g/kg trọng lượng cơ thể, trong 5 tuần) có hoặc không có bổ sung dầu thô. Bệnh gan và tổn thương cơ tim được giải thích là do sự gia tăng đáng kể (p < 0,05) các enzym sinh học gan và tim trong huyết thanh. Nồng độ creatinine và urê cũng tăng đáng kể trong nhóm ăn dầu chiên như một dấu hiệu của tổn thương thận. Nhưng tất cả các dấu ấn sinh học bao gồm triglycerid, cholesterol lipoprotein tỷ trọng thấp (LDL-C) đều giảm đáng kể (p < 0,05) ở nhóm chuột được tập thể dục. Những thay đổi về chuyển hóa này được chứng minh bằng nghiên cứu mô học các cơ quan tương ứng. Do đó, mọi người nên tránh tiêu thụ dầu chiên đi chiên lại nhiều lần thay vì dầu tươi mặc dù việc tập thể dục thường xuyên đã được phát hiện là có thể giải quyết những bất thường về chuyển hóa này. CÁC ỨNG DỤNG THỰC TẾ: Tập thể dục là một hoạt động phổ biến để giảm sự khởi phát của một số bất thường về chuyển hóa, ví dụ như bệnh tim mạch, bệnh gan nhiễm mỡ không do rượu, bệnh thận mãn tính và cũng ngăn ngừa tổn thương tế bào và mô khác nhau. Việc chiên dầu thực vật nhiều lần không chỉ làm giảm giá trị dinh dưỡng của nó mà còn tạo ra các axit béo tự do và các hợp chất độc hại khác. Nghiên cứu hiện tại cho thấy việc tiêu thụ dầu thực vật được đun nóng nhiều lần gây ra tổn thương ở tim, gan, thận và ruột non do các sản phẩm oxy hóa. Kết quả là, mức độ các dấu ấn sinh học của các cơ quan phản ứng này được phát hiện là tăng lên. Nhưng tập thể dục thường xuyên đã cải thiện những tác động có hại này. Vì vậy, về mặt quan tâm đến sức khỏe cộng đồng, tập thể dục thường xuyên là một trong những cách tốt nhất để giữ cho cơ thể khỏe mạnh, đặc biệt là những người đang tiêu thụ dầu chiên ngập."} +{"idx": 1870285, "en": "High-field nuclear magnetic resonance (NMR) has proven to be a valuable tool to analyze petroleum products but has never acquired widespread acceptance in routine work in oil refinery due to the high cost. The advent of compact, reliable, and affordable cryogen-free low-field benchtop NMR spectrometers suggest a possible role of such instrument for a variety of uses in refining spanning from routine lab work applications to online analysis. In this work, we report the development of a novel benchtop NMR application to estimate the API gravity, sulfur content, total acidity number (TAN), and distillation yields of crude petroleum from 60 MHz 1 H NMR spectra and partial least square (PLS) regression models. The results obtained on a dataset of over 170 crude oil samples and tested with external validation set show performances in terms of root mean square standard error of prediction (RMSEP) matching the analytical methods' ASTM precision. This novel benchtop NMR and multivariate analysis application can thus be used in typical refinery laboratories to provide within minutes and without physical distillation, reliable estimates of the most important properties usually measured on the crude petroleum during refining. The method proves to be a fast, cheaper alternative to the current analytical options providing readily available data for managing crude oil and has high potential to be further exploited for online application.", "gemini": "Cộng hưởng từ hạt nhân từ trường cao (NMR) đã được chứng minh là một công cụ hữu ích để phân tích các sản phẩm dầu mỏ nhưng chưa bao giờ được chấp nhận rộng rãi trong công việc thường quy tại nhà máy lọc dầu do chi phí cao. Sự ra đời của máy quang phổ NMR để bàn từ trường thấp không chứa cryogen nhỏ gọn, đáng tin cậy và giá cả phải chăng cho thấy vai trò tiềm năng của thiết bị như vậy đối với nhiều mục đích sử dụng trong quá trình lọc dầu, từ các ứng dụng công việc phòng thí nghiệm thường quy đến phân tích trực tuyến. Trong công trình này, chúng tôi báo cáo về sự phát triển của một ứng dụng NMR để bàn mới để ước tính trọng lượng API, hàm lượng lưu huỳnh, số axit toàn phần (TAN) và sản lượng chưng cất của dầu thô từ phổ NMR 1 H 60 MHz và các mô hình hồi quy bình phương toàn phần (PLS). Các kết quả thu được trên một tập dữ liệu gồm hơn 170 mẫu dầu thô và được kiểm tra bằng bộ xác nhận bên ngoài cho thấy hiệu suất về độ lệch chuẩn trung bình bình phương của lỗi dự đoán (RMSEP) phù hợp với độ chính xác của phương pháp phân tích ASTM. Do đó, ứng dụng phân tích đa biến và NMR để bàn mới này có thể được sử dụng trong các phòng thí nghiệm lọc dầu thông thường để cung cấp trong vòng vài phút và không cần chưng cất vật lý, các ước tính đáng tin cậy về các tính chất quan trọng nhất thường được đo trên dầu thô trong quá trình lọc dầu. Phương pháp này chứng tỏ là một phương pháp thay thế nhanh chóng và rẻ hơn cho các lựa chọn phân tích hiện tại, cung cấp dữ liệu có sẵn để quản lý dầu thô và có tiềm năng cao để được khai thác hơn nữa cho ứng dụng trực tuyến."} +{"idx": 1870286, "en": "BACKGROUND The effect of multiple co-pigments on the color intensification of mulberry anthocyanins (ACs) using spectroscopic techniques in combination with a molecular docking study was studied. The hyperchromicity of ACs co-pigmented with chlorogenic acid (CH) and quercetin (Q) blends was measured and their color stability in liquid and encapsulated particle models was evaluated. RESULTS Multiple co-pigments exhibited higher hyperchromicity, pKH -values, and heat-stability than their individual counterparts. Surflex-docking findings confirmed that stronger binding occurred between multiple ligands and AC than single ones due to their extra -OH, -COOH groups, and delocalization systems. The binding was allowed by increased H-bonding, van der Waals forces, and π-π sites by the extra groups of the multiple co-pigments with AC in aqueous juice and whey particle-based models. CONCLUSION This is the first report of the ternary mixture of phenolic acid-flavonol-anthocyanin which could be used as promising food red-colorants. © 2020 Society of Chemical Industry.", "gemini": "BỐI CẢNH Tác dụng của nhiều sắc tố đồng thời đối với cường độ màu của anthocyanin dâu tằm (AC) bằng các kỹ thuật quang phổ kết hợp với nghiên cứu ghép nối phân tử đã được nghiên cứu. Độ tăng sắc của AC đồng sắc tố với axit chlorogenic (CH) và hỗn hợp quercetin (Q) đã được đo và độ ổn định màu của chúng trong các mô hình hạt lỏng và hạt được đóng gói đã được đánh giá. KẾT QUẢ Nhiều sắc tố đồng thời biểu hiện độ tăng sắc, giá trị pKH và độ bền nhiệt cao hơn so với các sắc tố riêng lẻ của chúng. Các phát hiện ghép nối Surflex xác nhận rằng liên kết mạnh hơn xảy ra giữa nhiều phối tử và AC hơn là các phối tử đơn do các nhóm -OH, -COOH và hệ thống phi cục bộ hóa của chúng. Liên kết được cho phép bởi liên kết H gia tăng, lực van der Waals và các vị trí π-π bởi các nhóm bổ sung của nhiều sắc tố đồng thời với AC trong mô hình nước ép và mô hình dựa trên hạt váng sữa. KẾT LUẬN Đây là báo cáo đầu tiên về hỗn hợp ba thành phần axit phenolic-flavonol-anthocyanin có thể được sử dụng như một chất tạo màu đỏ thực phẩm đầy hứa hẹn. © 2020 Hội Công nghiệp Hóa học."} +{"idx": 1870287, "en": "AIM To explore nurses' experiences in natural disaster response. BACKGROUND Nurses are key to disaster response. There is a growing body of qualitative research exploring this emerging nursing issue. However, there is a need to synthesise and summarise this body of knowledge to identify the overarching elements of how nurses experience working in disaster situations to reflect on their experiences so that we may help shape future clinical practice, research and education. DESIGN Qualitative meta-synthesis. METHOD Following PROSPERO guidelines (Moher et al., 2015), an exhaustive and systematic literature search and quality appraisal was undertaken in December 2019 to reveal nurses' experiences during natural disaster response. Sandelowski and Barroso's systematic retrieval, analysis and interpretation of findings method was used to produce a meta-summary of findings from 10 papers evaluating experiences across 9 disasters. A meta-aggregation was used to synthesise the findings from the studies and was methodically quality assessed with PRISMA and CASP. RESULTS Our findings aggregated data from 42 sub-themes, into the following four themes to capture nurses' experiences after responding to disasters. These included agile response; leadership and innovative problem solving; building resilience; positive communication and need for psychological/emotional support. DISCUSSION This meta-synthesis provides evidence to illustrate nurses' resilience and leadership capabilities as means to manage and perceive their disaster relief response. Factors such as emotional intelligence, capacity to react to changing situations, to manage scant resources in extreme situations were highlighted in nurses practising in highly stressful environments. Managers can use these examples to support ways to improve disaster management policies, but also, to engage in support for their staff. RELEVANCE TO CLINICAL PRACTICE The role of nursing staff in disaster rescue is receiving significant attention. Understanding nurses' experiences during disaster rescue can help future leaders to improve capacity to respond and nursing preparedness through education, training and management, but also for continuing emotional support after the event.", "gemini": "MỤC ĐÍCH Khám phá kinh nghiệm của điều dưỡng trong ứng phó với thảm họa thiên nhiên. BỐI CẢNH Điều dưỡng là chìa khóa trong ứng phó với thảm họa. Có một khối lượng lớn các nghiên cứu định tính đang khám phá vấn đề điều dưỡng mới nổi này. Tuy nhiên, cần phải tổng hợp và tóm tắt khối kiến thức này để xác định các yếu tố bao quát về cách điều dưỡng trải nghiệm làm việc trong các tình huống thảm họa để phản ánh kinh nghiệm của họ, từ đó chúng ta có thể giúp định hình thực hành lâm sàng, nghiên cứu và giáo dục trong tương lai. THIẾT KẾ Tổng hợp siêu định tính. PHƯƠNG PHÁP Theo hướng dẫn của PROSPERO (Moher et al., 2015), một tìm kiếm tài liệu toàn diện và có hệ thống và đánh giá chất lượng đã được tiến hành vào tháng 12 năm 2019 để tìm hiểu kinh nghiệm của điều dưỡng trong quá trình ứng phó với thảm họa thiên nhiên. Phương pháp truy xuất, phân tích và diễn giải có hệ thống của Sandelowski và Barroso đã được sử dụng để tạo ra một bản tóm tắt siêu dữ liệu từ 10 bài báo đánh giá kinh nghiệm trong 9 thảm họa. Một tổng hợp siêu dữ liệu đã được sử dụng để tổng hợp các phát hiện từ các nghiên cứu và được đánh giá chất lượng một cách có phương pháp với PRISMA và CASP. KẾT QUẢ Phát hiện của chúng tôi tổng hợp dữ liệu từ 42 chủ đề phụ thành bốn chủ đề sau đây để nắm bắt kinh nghiệm của điều dưỡng sau khi ứng phó với thảm họa. Những điều này bao gồm phản ứng nhanh nhẹn; khả năng lãnh đạo và giải quyết vấn đề sáng tạo; xây dựng khả năng phục hồi; giao tiếp tích cực và nhu cầu hỗ trợ về mặt tâm lý/cảm xúc. THẢO LUẬN Tổng hợp siêu dữ liệu này cung cấp bằng chứng minh họa khả năng phục hồi và khả năng lãnh đạo của điều dưỡng như một phương tiện để quản lý và nhận thức phản ứng cứu trợ thảm họa của họ. Các yếu tố như trí tuệ cảm xúc, khả năng phản ứng với các tình huống thay đổi, quản lý nguồn lực khan hiếm trong các tình huống khắc nghiệt đã được nêu bật ở những điều dưỡng hành nghề trong môi trường căng thẳng cao độ. Những người quản lý có thể sử dụng những ví dụ này để hỗ trợ các cách cải thiện chính sách quản lý thảm họa, nhưng cũng để tham gia hỗ trợ cho nhân viên của họ. SỰ LIÊN QUAN ĐẾN THỰC HÀNH LÂM SÀNG Vai trò của nhân viên điều dưỡng trong cứu hộ thảm họa đang nhận được sự quan tâm đáng kể. Việc hiểu được kinh nghiệm của điều dưỡng trong quá trình cứu hộ thảm họa có thể giúp các nhà lãnh đạo tương lai cải thiện khả năng ứng phó và sự chuẩn bị của điều dưỡng thông qua giáo dục, đào tạo và quản lý, nhưng cũng để tiếp tục hỗ trợ về mặt cảm xúc sau sự kiện."} +{"idx": 1870288, "en": "We purified and compared the polyphenol oxidase (PPO) isoenzymes present in \"Xushu 22,\" a sweet potato. A membrane-bound form (mPPO) and two soluble forms (sPPO1 and sPPO2) were identified and purified using ammonium sulphate precipitation, ion exchange chromatography, gel filtration chromatography, and mass spectrometer. The three PPO isoforms were characterized enzymatically. The specific activity of mPPO was significantly higher than that of the two sPPO isoforms, being 24.55- and 13.89-fold higher than sPPO1 and sPPO2. The preferred substrates for mPPO and the two sPPOs were catechol and chlorogenic acid, respectively. They can be efficiently and safely applied to phenolic wastewater treatment after being immobilized. Both mPPO and the two sPPOs were rapidly inactivated under acid or base conditions and were unstable at 65°C. The most effective inhibitors of mPPO, sPPO1, and sPPO2 were glutathione, ascorbic acid, and L-cysteine, respectively. PRACTICAL APPLICATIONS: According to incomplete statistics, about 5% of sweet potatoes in china are wasted due to enzymatic browning every year. PPO was regarded as a key molecule contributing to enzymatic browning in fruits and vegetables during ripening, processing, and storage and responsible for economic and industrial loss. It's great importance to characterize the PPO from \"Xushu 22\" sweet potato and provide data on its inactivation. The three PPO isoforms were purified and identified by chromatography and mass spectrometer. This study will provide useful informations to have a better command of PPO from \"Xushu 22\" sweet potato and give ideals to solve the browning of sweet potato.", "gemini": "Chúng tôi đã tinh chế và so sánh các isoenzym polyphenol oxidase (PPO) có trong \"Xushu 22\", một loại khoai lang. Một dạng liên kết màng (mPPO) và hai dạng hòa tan (sPPO1 và sPPO2) đã được xác định và tinh chế bằng cách kết tủa amoni sunfat, sắc ký trao đổi ion, sắc ký gel lọc và phổ khối. Ba dạng đồng phân PPO đã được đặc trưng về mặt enzim. Hoạt tính đặc hiệu của mPPO cao hơn đáng kể so với hai dạng đồng phân sPPO, cao hơn 24,55 và 13,89 lần so với sPPO1 và sPPO2. Các chất nền ưa thích cho mPPO và hai sPPO lần lượt là catechol và axit chlorogenic. Chúng có thể được áp dụng hiệu quả và an toàn để xử lý nước thải phenol sau khi được cố định. Cả mPPO và hai sPPO đều nhanh chóng bị bất hoạt trong điều kiện axit hoặc bazơ và không ổn định ở 65°C. Các chất ức chế hiệu quả nhất của mPPO, sPPO1 và sPPO2 lần lượt là glutathione, axit ascorbic và L-cystein. ỨNG DỤNG THỰC TẾ: Theo thống kê chưa đầy đủ, khoảng 5% khoai lang ở Trung Quốc bị lãng phí do hiện tượng thâm đen do enzym gây ra hàng năm. PPO được coi là một phân tử chính góp phần vào quá trình thâm đen do enzym ở trái cây và rau quả trong quá trình chín, chế biến và bảo quản và gây ra tổn thất về kinh tế và công nghiệp. Việc xác định đặc điểm của PPO từ khoai lang \"Xushu 22\" và cung cấp dữ liệu về quá trình bất hoạt của nó có ý nghĩa rất lớn. Ba dạng đồng phân PPO đã được tinh chế và xác định bằng sắc ký và phổ khối. Nghiên cứu này sẽ cung cấp thông tin hữu ích để hiểu rõ hơn về PPO từ khoai lang \"Xushu 22\" và đưa ra lý tưởng để giải quyết tình trạng thâm đen của khoai lang."} +{"idx": 1870289, "en": "Annual outbreaks of seasonal influenza cause a substantial health burden. The aim of this study was to compare patient demographic/clinical data in two influenza patient groups presenting to hospital; those requiring O2 or critical care admission and those requiring less intensive treatment. The study was conducted from 1 December 2017 until 1 April 2019 at a district general hospital in East London. Patient demographic and clinical information was collected for all patients who had tested influenza positive by near-patient testing. χ2 test was used for categorical variables to see if there were significant differences for those admitted and the Wilcoxon rank-sum test to compare the length of inpatient stay. Of 127 patients, 56 (44.1%) required oxygen or critical care. There were significant increases in National Early Warning Score (NEWS) observations (P %3C .001), Charlson comorbidity index (P = .049), length of inpatient stay (P %3C .001), and a strong association with increasing age (P = .066) when the more intensive treatment group was compared with the less intensive treatment group. A total of 13 (18.3%) of 71 patients not requiring oxygen or critical care were not admitted to the hospital. Following rapid influenza testing, NEWS scores, comorbidities, and age should be incorporated into a decision tool in Accident and Emergency to aid hospital admission or discharge decisions.", "gemini": "Các đợt bùng phát cúm theo mùa hàng năm gây ra gánh nặng đáng kể cho sức khỏe. Mục đích của nghiên cứu này là so sánh dữ liệu nhân khẩu học/lâm sàng của bệnh nhân trong hai nhóm bệnh nhân cúm đến bệnh viện; những người cần nhập viện cấp cứu hoặc chăm sóc đặc biệt và những người cần điều trị ít chuyên sâu hơn. Nghiên cứu được thực hiện từ ngày 1 tháng 12 năm 2017 đến ngày 1 tháng 4 năm 2019 tại một bệnh viện đa khoa quận ở Đông London. Thông tin nhân khẩu học và lâm sàng của bệnh nhân được thu thập đối với tất cả những bệnh nhân đã xét nghiệm dương tính với cúm bằng xét nghiệm gần bệnh nhân. Thử nghiệm χ2 được sử dụng cho các biến phân loại để xem có sự khác biệt đáng kể nào đối với những bệnh nhân được nhập viện và thử nghiệm tổng Wilcoxon để so sánh thời gian nằm viện. Trong số 127 bệnh nhân, 56 (44,1%) cần thở oxy hoặc chăm sóc đặc biệt. Có sự gia tăng đáng kể về các quan sát về Điểm cảnh báo sớm quốc gia (NEWS) (P %3C .001), chỉ số bệnh đi kèm Charlson (P = .049), thời gian nằm viện (P %3C .001) và mối liên hệ chặt chẽ với việc tăng tuổi (P = .066) khi nhóm điều trị chuyên sâu hơn được so sánh với nhóm điều trị ít chuyên sâu hơn. Tổng cộng 13 (18,3%) trong số 71 bệnh nhân không cần thở oxy hoặc chăm sóc đặc biệt không được nhập viện. Sau khi xét nghiệm cúm nhanh, điểm NEWS, bệnh đi kèm và tuổi nên được đưa vào công cụ hỗ trợ ra quyết định tại Khoa Cấp cứu và Tai nạn để hỗ trợ các quyết định nhập viện hoặc xuất viện."} +{"idx": 1870290, "en": "BACKGROUND AND AIM In addition to index colonoscopy findings, demographic parameters including age are associated with the risk of metachronous advanced colorectal neoplasia. Here, we aimed to develop a risk scoring model for predicting advanced colorectal neoplasia (ACRN) during surveillance using a combination of clinical factors and index colonoscopy findings. METHODS Patients who underwent the removal of one or more adenomas and surveillance colonoscopy were included. A risk scoring model for ACRN was developed using the Cox proportional hazard model. Surveillance interval was determined as a time point exceeding 4% of the cumulative ACRN incidence in each risk group. RESULTS Of 9591 participants, 4725 and 4866 were randomly allocated to the derivation and validation cohorts, respectively. Age, abdominal obesity, advanced adenoma, and ≥ 3 adenomas at index colonoscopy were identified as risk factors for metachronous ACRN. Based on the regression coefficients, point scores were assigned as follows: age, 1 point (per 1 year); abdominal obesity, 10 points; advanced adenoma, 10 points; and ≥ 3 adenomas, 15 points. Patients were classified into high-risk (≥ 80 points), moderate-risk (50-79 points), and low-risk (30-49 points) groups. In the validation cohort, the high-risk and moderate-risk groups showed a higher risk of ACRN than the low-risk group (hazard ratio [95% confidence interval]: 7.11 [4.10-12.32] and 1.58 [1.09-2.30], respectively). Two-, 4-, and 5-year surveillance intervals were recommended for the high-risk, moderate-risk, and low-risk groups, respectively. CONCLUSIONS Our proposed model may facilitate effective risk stratification of ACRN during surveillance and the determination of appropriate surveillance intervals.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Ngoài các phát hiện nội soi đại tràng, các thông số nhân khẩu học bao gồm tuổi có liên quan đến nguy cơ mắc bệnh tân sinh đại trực tràng tiến triển không đồng thời. Ở đây, chúng tôi đặt mục tiêu phát triển một mô hình chấm điểm rủi ro để dự đoán bệnh tân sinh đại trực tràng tiến triển (ACRN) trong quá trình theo dõi bằng cách kết hợp các yếu tố lâm sàng và các phát hiện nội soi đại tràng. PHƯƠNG PHÁP Các bệnh nhân đã cắt bỏ một hoặc nhiều khối u tuyến và nội soi đại tràng theo dõi đã được đưa vào. Một mô hình chấm điểm rủi ro cho ACRN đã được phát triển bằng cách sử dụng mô hình nguy cơ tỷ lệ Cox. Khoảng thời gian theo dõi được xác định là thời điểm vượt quá 4% tỷ lệ mắc ACRN tích lũy trong mỗi nhóm rủi ro. KẾT QUẢ Trong số 9591 người tham gia, 4725 và 4866 được phân bổ ngẫu nhiên vào các nhóm phái sinh và xác nhận. Tuổi, béo bụng, khối u tuyến tiến triển và ≥ 3 khối u tuyến ở nội soi đại tràng được xác định là các yếu tố rủi ro đối với ACRN không đồng thời. Dựa trên các hệ số hồi quy, điểm số được chỉ định như sau: tuổi, 1 điểm (mỗi 1 năm); béo bụng, 10 điểm; khối u tuyến tiến triển, 10 điểm; và ≥ 3 khối u tuyến, 15 điểm. Bệnh nhân được phân loại vào các nhóm rủi ro cao (≥ 80 điểm), rủi ro trung bình (50-79 điểm) và rủi ro thấp (30-49 điểm). Trong nhóm xác nhận, nhóm rủi ro cao và nhóm rủi ro trung bình có nguy cơ mắc ACRN cao hơn nhóm rủi ro thấp (tỷ lệ nguy cơ [khoảng tin cậy 95%]: 7,11 [4,10-12,32] và 1,58 [1,09-2,30], tương ứng). Các khoảng thời gian theo dõi hai, bốn và năm năm được khuyến nghị cho các nhóm rủi ro cao, rủi ro trung bình và rủi ro thấp. KẾT LUẬN Mô hình chúng tôi đề xuất có thể tạo điều kiện thuận lợi cho việc phân tầng rủi ro hiệu quả của ACRN trong quá trình theo dõi và xác định các khoảng thời gian theo dõi thích hợp."} +{"idx": 1870291, "en": "CD46 (also known as membrane cofactor protein), which is a member of the membrane-bound complement regulatory protein family, has been reported to cause cancer cells to escape complement-dependent cytotoxicity. However, the association between CD46 polymorphisms and the risk of hepatocellular carcinoma (HCC) has not been investigated. This two-stage association study was conducted to assess the relationship between the tagging single nucleotide polymorphisms (tagSNPs) of CD46 and HCC risk and prognosis. A series of functional analyses were performed to study the underlying mechanisms. Among the eight tagSNPs, rs2796267 (P = .003) and rs2796268 (P = .011) were found to modify HCC risk in the discovery set. Only rs2796267 (P < .0001) was confirmed to be associated with HCC susceptibility in the validation set. Compared with the wild-type AA genotype, the GG genotype significantly increased the HCC risk (adjusted odds ratio [OR] = 2.03; 95% confidence interval [CI], 1.34-3.08; P = .001). Moreover, subgroups analysis suggested a positive correlation among male and younger patients, especially among drinkers, smokers, and hepatitis B surface antigen-positive individuals. In functional analyses, we found that the rs2796267 G allele in the promoter region of CD46 could increase the expression of CD46 by affecting the binding affinity of STAT5a. Furthermore, Cox regression analysis revealed that the rs2796267 AG/GG genotype was significantly associated with worse prognosis of resected patients with HCC (hazard ratio = 2.27; 95% CI, 1.27-4.05; P = .006). These results suggest that the CD46 rs2796267 polymorphism may contribute to susceptibility and prognosis of HCC by altering promoter activity.", "gemini": "CD46 (còn được gọi là protein đồng yếu tố màng), một thành viên của họ protein điều hòa bổ sung gắn màng, đã được báo cáo là nguyên nhân khiến tế bào ung thư thoát khỏi độc tính phụ thuộc bổ sung. Tuy nhiên, mối liên quan giữa các đa hình CD46 và nguy cơ mắc ung thư biểu mô tế bào gan (HCC) vẫn chưa được nghiên cứu. Nghiên cứu liên kết hai giai đoạn này được tiến hành để đánh giá mối quan hệ giữa các đa hình nucleotide đơn đánh dấu (tagSNP) của CD46 và nguy cơ và tiên lượng của HCC. Một loạt các phân tích chức năng đã được thực hiện để nghiên cứu các cơ chế cơ bản. Trong số tám tagSNP, rs2796267 (P = 0,003) và rs2796268 (P = 0,011) được phát hiện là làm thay đổi nguy cơ mắc HCC trong tập hợp phát hiện. Chỉ có rs2796267 (P <0,0001) được xác nhận có liên quan đến nguy cơ mắc HCC trong tập hợp xác nhận. So với kiểu gen AA kiểu hoang dã, kiểu gen GG làm tăng đáng kể nguy cơ mắc HCC (tỷ lệ nguy cơ điều chỉnh [OR] = 2,03; khoảng tin cậy 95% [CI], 1,34-3,08; P = 0,001). Hơn nữa, phân tích các nhóm phụ cho thấy có mối tương quan tích cực giữa nam giới và bệnh nhân trẻ tuổi, đặc biệt là ở những người uống rượu, hút thuốc và những người có kháng nguyên bề mặt viêm gan B dương tính. Trong các phân tích chức năng, chúng tôi phát hiện ra rằng alen G rs2796267 trong vùng khởi động của CD46 có thể làm tăng biểu hiện của CD46 bằng cách ảnh hưởng đến ái lực liên kết của STAT5a. Hơn nữa, phân tích hồi quy Cox cho thấy kiểu gen rs2796267 AG / GG có liên quan đáng kể đến tiên lượng xấu hơn của bệnh nhân cắt bỏ HCC (tỷ lệ nguy cơ = 2,27; 95% CI, 1,27-4,05; P = 0,006). Những kết quả này cho thấy rằng đa hình CD46 rs2796267 có thể góp phần vào tính nhạy cảm và tiên lượng của HCC bằng cách làm thay đổi hoạt động của vùng khởi động."} +{"idx": 1870292, "en": "Commercial LiAlH4 can be used in catalytic quantities in the hydrogenation of imines to amines with H2 . Combined experimental and theoretical investigations give deeper insight in the mechanism and identifies the most likely catalytic cycle. Activity is lost when Li in LiAlH4 is exchanged for Na or K. Exchanging Al for B or Ga also led to dramatically reduced activities. This indicates a heterobimetallic mechanism in which cooperation between Li and Al is crucial. Potential intermediates on the catalytic pathway have been isolated from reactions of MAlH4 (M=Li, Na, K) and different imines. Depending on the imine, double, triple or quadruple imine insertion has been observed. Prolonged reaction of LiAlH4 with PhC(H)=NtBu led to a side-reaction and gave the double insertion product LiAlH2 [N]2 ([N]=N(tBu)CH2 Ph) which at higher temperature reacts further by ortho-metallation of the Ph ring. A DFT study led to a number of conclusions. The most likely catalyst for hydrogenation of PhC(H)=NtBu with LiAlH4 is LiAlH2 [N]2 . Insertion of a third imine via a heterobimetallic transition state has a barrier of +23.2 kcal mol-1 (ΔH). The rate-determining step is hydrogenolysis of LiAlH[N]3 with H2 with a barrier of +29.2 kcal mol-1 . In agreement with experiment, replacing Li for Na (or K) and Al for B (or Ga) led to higher calculated barriers. Also, the AlH4 - anion showed very high barriers. Calculations support the experimentally observed effects of the imine substituents at C and N: the lowest barriers are calculated for imines with aryl-substituents at C and alkyl-substituents at N.", "gemini": "LiAlH4 thương mại có thể được sử dụng với số lượng xúc tác để hydro hóa imin thành amin với H2. Các nghiên cứu kết hợp thực nghiệm và lý thuyết cung cấp cái nhìn sâu sắc hơn về cơ chế và xác định chu kỳ xúc tác có khả năng xảy ra nhất. Hoạt động bị mất khi Li trong LiAlH4 được trao đổi với Na hoặc K. Việc trao đổi Al cho B hoặc Ga cũng dẫn đến hoạt động giảm đáng kể. Điều này chỉ ra một cơ chế dị kim loại trong đó sự hợp tác giữa Li và Al là rất quan trọng. Các chất trung gian tiềm ẩn trên con đường xúc tác đã được phân lập từ phản ứng của MAlH4 (M = Li, Na, K) và các imin khác nhau. Tùy thuộc vào imin, đã quan sát thấy sự chèn imin đôi, ba hoặc bốn. Phản ứng kéo dài của LiAlH4 với PhC (H) = NtBu dẫn đến phản ứng phụ và tạo ra sản phẩm chèn đôi LiAlH2 [N] 2 ([N] = N (tBu) CH2 Ph) phản ứng ở nhiệt độ cao hơn nữa bằng cách ortho-kim loại hóa vòng Ph. Một nghiên cứu DFT đã đưa ra một số kết luận. Xúc tác có khả năng nhất cho quá trình hydro hóa PhC (H) = NtBu với LiAlH4 là LiAlH2 [N] 2. Việc chèn một imin thứ ba thông qua trạng thái chuyển tiếp dị kim loại có rào cản là + 23,2 kcal · mol-1 (ΔH). Bước xác định tốc độ là quá trình thủy phân của LiAlH [N] 3 với H2 với rào cản là + 29,2 kcal · mol-1. Phù hợp với thí nghiệm, việc thay thế Li bằng Na (hoặc K) và Al bằng B (hoặc Ga) dẫn đến rào cản tính toán cao hơn. Ngoài ra, anion AlH4 - cho thấy các rào cản rất cao. Các tính toán hỗ trợ các hiệu ứng quan sát được trên thực nghiệm của các chất thế imin tại C và N: các rào cản thấp nhất được tính toán cho các imin có chất thế aryl tại C và chất thế alkyl tại N."} +{"idx": 1870293, "en": "Osteogenic differentiation (OD) of bone marrow mesenchymal stem cells (BMSCs) is critically important for mitigation of osteoporosis. Glucocorticoids (GCs) are extensively used for treating chronic inflammation, although long-term exposure to GCs is capable of triggering osteoporosis. microRNAs (miRNAs) have been reported to play a critical role in bone diseases. In the present study, we treated BMSCs with dexamethasone (DEX) during OD to stimulate GC-mediated osteoporosis. Microarray and quantitative polymerase chain reaction (Q-PCR) assays demonstrated that miR-199a was upregulated during OD of BMSCs, while DEX treatment caused a significant reduction in miR-199a. Alkaline phosphatase (ALP) activity, Alizarin red (AR) staining, and Q-PCR were applied to assess the role of miRNA-199a overexpression in DEX-triggered OD inhibition. miR-199a was able to rescue OD and ALP activity, which were inhibited by DEX. Additionally, we observed that ALP, BMP2, COL1A1, and Runx2 were increased after transfection of miRNA-199a mimics. Furthermore, we confirmed that miRNA-199a facilitates OD of BMSCs through direct inhibition of Klotho protein and messenger RNA expression affecting the downstream fibroblast growth factor receptor 1/extracellular-signal-regulated kinase and Janus kinase 1/signal transducer and activator of transcription 1 pathways. This study indicates that miR-199a plays a critical role in preventing GC-mediated osteoblast differentiation and may function as a promising miRNA biomarker for osteoporosis.", "gemini": "Sự biệt hóa tạo xương (OD) của các tế bào gốc trung mô tủy xương (BMSC) có vai trò cực kỳ quan trọng trong việc giảm thiểu loãng xương. Glucocorticoid (GC) được sử dụng rộng rãi để điều trị viêm mạn tính, mặc dù tiếp xúc lâu dài với GC có khả năng gây ra loãng xương. MicroRNA (miRNA) được báo cáo đóng vai trò quan trọng trong các bệnh về xương. Trong nghiên cứu hiện tại, chúng tôi đã xử lý BMSC bằng dexamethasone (DEX) trong quá trình OD để kích thích loãng xương do GC. Các xét nghiệm mảng vi mô và phản ứng chuỗi polymerase định lượng (Q-PCR) chứng minh rằng miR-199a được điều hòa tăng lên trong quá trình OD của BMSC, trong khi điều trị DEX gây ra sự giảm đáng kể miR-199a. Hoạt tính phosphatase kiềm (ALP), nhuộm đỏ Alizarin (AR) và Q-PCR được áp dụng để đánh giá vai trò của sự biểu hiện quá mức miRNA-199a trong quá trình ức chế OD do DEX gây ra. miR-199a có khả năng phục hồi hoạt động OD và ALP, bị ức chế bởi DEX. Ngoài ra, chúng tôi quan sát thấy rằng ALP, BMP2, COL1A1 và Runx2 tăng lên sau khi chuyển gen bắt chước miRNA-199a. Hơn nữa, chúng tôi xác nhận rằng miRNA-199a tạo thuận lợi cho OD của BMSC thông qua quá trình ức chế trực tiếp protein Klotho và biểu hiện RNA thông tin ảnh hưởng đến các con đường thụ thể yếu tố tăng trưởng nguyên bào sợi hạ lưu 1 / kinase điều hòa tín hiệu ngoại bào và Janus kinase 1 / bộ chuyển đổi tín hiệu và kích hoạt phiên mã 1. Nghiên cứu này chỉ ra rằng miR-199a đóng vai trò quan trọng trong việc ngăn ngừa biệt hóa tế bào tạo xương do GC và có thể hoạt động như một dấu ấn sinh học miRNA đầy hứa hẹn cho bệnh loãng xương."} +{"idx": 1870294, "en": "Mesenchymal stem cells (MSCs) are a subset of multifunctional stem cells with self-renewal and multidirectional differentiation properties that play a pivotal role in tumor progression. MSCs are reported to exert biological functions by secreting specialized vesicles, known as exosomes, with tumor cells. Exosomes participate in material and information exchange between cells and are crucial in multiple physiological and pathological processes. This study provides a comprehensive overview of the roles, mechanisms of action and sources of MSC exosomes in hematological malignancies, and different tumor types.", "gemini": "Tế bào gốc trung mô (MSC) là một phân nhóm của tế bào gốc đa chức năng có khả năng tự tái tạo và biệt hóa đa hướng, đóng vai trò trung tâm trong quá trình phát triển khối u. Người ta báo cáo rằng MSC phát huy chức năng sinh học bằng cách tiết ra các túi chuyên biệt, được gọi là exosome, với các tế bào khối u. Exosome tham gia vào quá trình trao đổi vật chất và thông tin giữa các tế bào và đóng vai trò quan trọng trong nhiều quá trình sinh lý và bệnh lý. Nghiên cứu này cung cấp một cái nhìn tổng quan toàn diện về vai trò, cơ chế hoạt động và nguồn gốc của exosome MSC trong các bệnh ác tính về huyết học và các loại khối u khác nhau."} +{"idx": 1870295, "en": "Sulfonamides are recommended as part of first-line therapy for most Nocardia infections, with trimethoprim-sulfamethoxazole (TMP-SMX) considered the drug of choice for susceptible isolates. However, in the case of central nervous system, disseminated disease, and other serious Nocardia infections, TMP-SMX should not be used as monotherapy. The preferred treatment for a patient unable to take TMP-SMX because of allergy or intolerance remains uncertain. Prior to the availability of TMP-SMX in 1973, other sulfonamides were mainstays of treatment. We describe a Nocardia infection successfully treated with sulfadiazine in a lung transplant recipient who could not tolerate TMP-SMX. A review of similar cases reported in the literature provides insight into the successful treatment of Nocardia infections with sulfonamide regimens not containing trimethoprim in transplant recipients and other immunocompromised hosts.", "gemini": "Sulfonamid được khuyến cáo là một phần của liệu pháp điều trị đầu tay cho hầu hết các trường hợp nhiễm Nocardia, trong đó trimethoprim-sulfamethoxazol (TMP-SMX) được coi là thuốc được lựa chọn cho các chủng nhạy cảm. Tuy nhiên, trong trường hợp hệ thần kinh trung ương, bệnh lan tỏa và các trường hợp nhiễm Nocardia nghiêm trọng khác, không nên sử dụng TMP-SMX làm đơn trị liệu. Phương pháp điều trị được ưu tiên cho bệnh nhân không thể dùng TMP-SMX do dị ứng hoặc không dung nạp vẫn chưa chắc chắn. Trước khi có TMP-SMX vào năm 1973, các sulfonamid khác là trụ cột của điều trị. Chúng tôi mô tả một trường hợp nhiễm Nocardia được điều trị thành công bằng sulfadiazine ở người nhận ghép phổi không dung nạp TMP-SMX. Việc xem xét các trường hợp tương tự được báo cáo trong tài liệu cung cấp cái nhìn sâu sắc về việc điều trị thành công các trường hợp nhiễm Nocardia bằng phác đồ sulfonamid không chứa trimethoprim ở những người nhận ghép tạng và những người bị suy giảm miễn dịch khác."} +{"idx": 1870296, "en": "OBJECTIVES To undertake a prospective multicentre national audit of penile prosthesis practice in the UK over a 3-year period. PATIENTS AND METHODS Data were submitted by urological surgeons as part of the British Association of Urological Surgeons Penile Prosthesis National Audit. Patients receiving a penile prosthesis (inflatable or malleable) were included as part of a prospective registry over a 3-year period. Data were validated and then analysed using a software package (Tableau). RESULTS A total of 1071 penile prosthesis procedures were included from 22 centres. The three commonest aetiological factors for erectile dysfunction were diabetes, prostate surgery and Peyronie's disease. Of the recorded data, inflatable penile prostheses were the commonest devices implanted, with 665 devices used (62.1%), whereas malleable prostheses accounted for 14.2% of the implants. Recorded intra-operative complications included urethral injury (0.7%, n = 7), corporal perforation (1.1%, n = 12) and cross-over (0.6%, n = 6). Known postoperative complications were recorded in 9.8% of patients (74/752), with the two most frequently reported being postoperative penile pain (n = 11) and scrotal haematoma (n = 14). CONCLUSION This baseline analysis is the largest prospective registry of penile prostheses procedures to date. The data show that, over the 3-year collection period in the UK, there are now fewer surgeons performing the procedure, together with a reduction in the number of centres. Peri-operative complications were infrequent, and the rate of implant abortion (e.g. as a result of urethral injury) was very low. Further follow-up data will be required to publish long-term outcomes and patient satisfaction.", "gemini": "MỤC TIÊU Thực hiện cuộc kiểm toán quốc gia đa trung tâm có triển vọng về thực hành đặt vật liệu thay thế dương vật ở Vương quốc Anh trong giai đoạn 3 năm. BỆNH NHÂN VÀ PHƯƠNG PHÁP Dữ liệu được các bác sĩ phẫu thuật tiết niệu gửi như một phần của cuộc kiểm toán quốc gia về vật liệu thay thế dương vật của Hiệp hội phẫu thuật tiết niệu Anh. Những bệnh nhân được đặt vật liệu thay thế dương vật (có thể bơm hơi hoặc uốn nắn được) đã được đưa vào như một phần của sổ đăng ký có triển vọng trong giai đoạn 3 năm. Dữ liệu được xác thực và sau đó được phân tích bằng một gói phần mềm (Tableau). KẾT QUẢ Tổng cộng có 1071 thủ thuật đặt vật liệu thay thế dương vật được đưa vào từ 22 trung tâm. Ba yếu tố nguyên nhân phổ biến nhất gây ra rối loạn cương dương là tiểu đường, phẫu thuật tuyến tiền liệt và bệnh Peyronie. Trong số các dữ liệu được ghi lại, vật liệu thay thế dương vật có thể bơm hơi là thiết bị được cấy ghép phổ biến nhất, với 665 thiết bị được sử dụng (62,1%), trong khi vật liệu thay thế có thể uốn nắn chiếm 14,2% số ca cấy ghép. Các biến chứng trong khi phẫu thuật được ghi nhận bao gồm chấn thương niệu đạo (0,7%, n = 7), thủng thể hang (1,1%, n = 12) và chéo (0,6%, n = 6). Các biến chứng hậu phẫu đã biết được ghi nhận ở 9,8% bệnh nhân (74/752), trong đó hai biến chứng được báo cáo thường xuyên nhất là đau dương vật sau phẫu thuật (n = 11) và tụ máu bìu (n = 14). KẾT LUẬN Phân tích cơ sở này là sổ đăng ký có triển vọng lớn nhất về các thủ thuật đặt vật liệu thay thế dương vật cho đến nay. Dữ liệu cho thấy, trong giai đoạn thu thập 3 năm ở Vương quốc Anh, hiện có ít bác sĩ phẫu thuật thực hiện thủ thuật hơn, cùng với việc giảm số lượng trung tâm. Các biến chứng quanh phẫu thuật rất ít và tỷ lệ phá thai cấy ghép (ví dụ như do chấn thương niệu đạo) rất thấp. Sẽ cần có thêm dữ liệu theo dõi để công bố kết quả dài hạn và sự hài lòng của bệnh nhân."} +{"idx": 1870297, "en": "Synthetic biology has the potential to seed research in impoverished countries. Teaching students and academics has to take into account reality - lessons from Honduras.", "gemini": "Sinh học tổng hợp có tiềm năng gieo mầm nghiên cứu ở các nước nghèo. Việc giảng dạy cho sinh viên và học giả phải tính đến thực tế - bài học từ Honduras."} +{"idx": 1870298, "en": "The World Health Organization has set ambitious viral hepatitis elimination targets; however, difficulties in identifying and engaging patients remain. The emergency visit is an opportunity for enhanced linkage to care (LTC). We assessed the effectiveness of an automated Emergency Department (ED) screening service in identifying patients with hepatitis C (HCV) and achieving LTC. A retrospective evaluation was undertaken, analysing the first 5000 patients screened through an automatic Australian service termed 'Screening Emergency Admissions at Risk of Chronic Hepatitis' (SEARCH). Screening was performed for those recommended in the Australian national testing policy, specifically overseas born (OB) and Aboriginal or Torres Strait Islanders (ATSI). Healthcare worker education, patient information materials and opt-out informed consent were used to test sera already collected for biochemistry assays. 5000 of 5801 (86.2%) consecutive eligible patients were screened (OB: 4778, ATSI: 222) from 14 093 ED presentations. HCV antibody was positive in 181 patients (3.6%); 51 (1.0%) were HCV RNA positive. Of 51 HCV RNA-positive patients, 12 were new diagnoses, 32 were 're-diagnoses' (aware but lost to follow-up [LTFU]), and 7 were previously known but treatment contraindicated. LTC was successful in 38 viraemic patients (7 deceased, 4 LTFU, 1 treatment ineligible and 1 declined). Of RNA-negative patients, 75 were previously treated and 49 had presumed spontaneous clearance. Opt-out consent was acceptable to all patients and staff involved. ED screening can lead to additional diagnosing and 're-diagnosing' of HCV, with high rates of LTC. Opt-out consent and automation removed major obstacles to testing.", "gemini": "Tổ chức Y tế Thế giới đã đặt ra các mục tiêu loại trừ viêm gan do vi-rút đầy tham vọng; tuy nhiên, vẫn còn khó khăn trong việc xác định và thu hút bệnh nhân. Chuyến thăm cấp cứu là cơ hội để tăng cường liên kết chăm sóc (LTC). Chúng tôi đã đánh giá hiệu quả của dịch vụ sàng lọc Khoa Cấp cứu (ED) tự động trong việc xác định bệnh nhân mắc bệnh viêm gan C (HCV) và đạt được LTC. Một đánh giá hồi cứu đã được thực hiện, phân tích 5000 bệnh nhân đầu tiên được sàng lọc thông qua một dịch vụ tự động của Úc có tên là 'Sàng lọc nhập viện cấp cứu có nguy cơ mắc bệnh viêm gan mãn tính' (SEARCH). Sàng lọc được thực hiện đối với những người được khuyến nghị trong chính sách xét nghiệm quốc gia của Úc, cụ thể là người sinh ra ở nước ngoài (OB) và người thổ dân hoặc dân đảo Torres Strait (ATSI). Giáo dục nhân viên y tế, tài liệu thông tin cho bệnh nhân và sự đồng ý được thông báo không tham gia đã được sử dụng để xét nghiệm huyết thanh đã được thu thập để xét nghiệm sinh hóa. 5000 trong số 5801 (86,2%) bệnh nhân đủ điều kiện liên tiếp đã được sàng lọc (OB: 4778, ATSI: 222) từ 14 093 lần khám cấp cứu. Kháng thể HCV dương tính ở 181 bệnh nhân (3,6%); 51 (1,0%) có RNA HCV dương tính. Trong số 51 bệnh nhân có RNA HCV dương tính, 12 trường hợp được chẩn đoán mới, 32 trường hợp được 'chẩn đoán lại' (biết nhưng bị mất theo dõi [LTFU]) và 7 trường hợp đã biết trước đó nhưng chống chỉ định điều trị. LTC đã thành công ở 38 bệnh nhân nhiễm vi-rút (7 trường hợp tử vong, 4 trường hợp LTFU, 1 trường hợp không đủ điều kiện điều trị và 1 trường hợp từ chối). Trong số những bệnh nhân có RNA âm tính, 75 bệnh nhân đã được điều trị trước đó và 49 bệnh nhân được cho là tự khỏi. Sự đồng ý không tham gia được tất cả bệnh nhân và nhân viên liên quan chấp nhận. Sàng lọc ED có thể dẫn đến chẩn đoán bổ sung và 'chẩn đoán lại' HCV, với tỷ lệ LTC cao. Sự đồng ý không tham gia và tự động hóa đã loại bỏ những trở ngại chính trong việc xét nghiệm."} +{"idx": 1870299, "en": "Coral reefs are facing increasing pressure from rising seawater temperatures and ocean acidification. Sponges have been proposed as possible winners in the face of climate change; however, little is known about the mechanisms underpinning their predicted tolerance. Here we assessed whether microbiome-mediated cross-generational acclimatization could enable the photosynthetic sponge Carteriospongia foliascens to survive under future climate scenarios. To achieve this, we first established the potential for vertical (cross-generational) transmission of symbionts. Sixty-four amplicon sequence variants accounting for >90% of the total C. foliascens microbial community were present across adult, larval and juvenile life stages, showing that a large proportion of the microbiome is vertically acquired and maintained. When C. foliascens were exposed to climate scenarios projected for 2050 and 2100, the host remained visibly unaffected (i.e. no necrosis/bleaching) and the overall microbiome was not significantly different amongst treatments in adult tissue, the respective larvae or recruits transplanted amongst climate treatments. However, indicator species analysis revealed that parental exposure to future climate scenarios altered the presence and abundance of a small suite of microbial taxa in the recruits, thereby revealing the potential for microbiome-mediated cross-generational acclimatization through both symbiont shuffling and symbiont switching within a vertically acquired microbiome.", "gemini": "Các rạn san hô đang phải đối mặt với áp lực ngày càng tăng từ nhiệt độ nước biển tăng và axit hóa đại dương. Bọt biển đã được đề xuất là những loài có thể chiến thắng trước biến đổi khí hậu; tuy nhiên, người ta biết rất ít về các cơ chế thúc đẩy khả năng chịu đựng được dự đoán của chúng. Ở đây, chúng tôi đánh giá xem liệu quá trình thích nghi liên thế hệ do vi sinh vật trung gian có thể giúp bọt biển quang hợp Carteriospongia foliascens tồn tại trong các kịch bản khí hậu trong tương lai hay không. Để đạt được điều này, trước tiên chúng tôi thiết lập khả năng truyền dọc (liên thế hệ) của các cộng sinh. Sáu mươi bốn biến thể trình tự amplicon chiếm> 90% tổng số cộng đồng vi sinh vật C. foliascens có mặt ở giai đoạn trưởng thành, ấu trùng và thiếu niên, cho thấy một tỷ lệ lớn vi sinh vật được tiếp nhận và duy trì theo chiều dọc. Khi C. foliascens tiếp xúc với các kịch bản khí hậu được dự đoán cho năm 2050 và 2100, vật chủ vẫn không bị ảnh hưởng rõ ràng (tức là không bị hoại tử / tẩy trắng) và hệ vi sinh vật tổng thể không có sự khác biệt đáng kể giữa các phương pháp điều trị ở mô trưởng thành, ấu trùng tương ứng hoặc những người được tuyển dụng được ghép giữa các phương pháp điều trị khí hậu. Tuy nhiên, phân tích các loài chỉ thị cho thấy việc tiếp xúc của cha mẹ với các kịch bản khí hậu trong tương lai đã làm thay đổi sự hiện diện và số lượng của một nhóm nhỏ các loài vi khuẩn ở những người được tuyển dụng, do đó tiết lộ tiềm năng thích nghi liên thế hệ do vi sinh vật trung gian thông qua cả sự xáo trộn cộng sinh và chuyển đổi cộng sinh trong một hệ vi sinh vật được tiếp nhận theo chiều dọc."} +{"idx": 1870300, "en": "Impaired glucose utilization has been implicated in the pathophysiology of neurodegenerative diseases. The neuroprotective effect of caffeic acid (CA) was investigated in the isolated rat brain by determining its ability to promote glucose uptake, mitigate redox imbalance, modulate purinergic and cholinergic activities, elemental distribution, and maintain tissue morphology. Isolated rat brains were incubated for 2 hours with glucose, CA and glucose, and metformin and glucose. There was an increased glucose uptake, glutathione level, superoxide dismutase, and catalase activities in brain tissues incubated with CA compared to the controls. Incubation with CA also led to significantly decreased levels of malondialdehyde, nitric oxide, acetylcholinesterase, butyrylcholinesterase, and ATPase activities. Electron microscopy (scanning electron microscopy and transmission electron microscopy) analysis portrayed a maintenance of tissue ultrastructural morphology in 2CA-incubated tissues as indicated by the intact synaptic vesicles, blood vessels, dendritic and neuronal network, mitochondria, and presynaptic membrane. CA also restored altered elemental levels in brain tissues. These results indicate the stimulatory potential of CA on brain glucose utilization with simultaneous neuroprotective activities.", "gemini": "Việc sử dụng glucose bị suy yếu có liên quan đến bệnh sinh của các bệnh thoái hóa thần kinh. Tác dụng bảo vệ thần kinh của axit caffeic (CA) đã được nghiên cứu trên não chuột bị cô lập bằng cách xác định khả năng thúc đẩy hấp thu glucose, giảm mất cân bằng oxy hóa khử, điều chỉnh hoạt động purinergic và cholinergic, phân bố nguyên tố và duy trì hình thái mô. Não chuột bị cô lập được ủ trong 2 giờ với glucose, CA và glucose và metformin và glucose. Có sự gia tăng hấp thu glucose, mức glutathione, hoạt động của superoxide dismutase và catalase trong các mô não được ủ với CA so với đối chứng. Việc ủ với CA cũng dẫn đến giảm đáng kể mức malondialdehyde, oxit nitric, acetylcholinesterase, butyrylcholinesterase và hoạt động của ATPase. Phân tích kính hiển vi điện tử (kính hiển vi điện tử quét và kính hiển vi điện tử truyền qua) mô tả việc duy trì hình thái siêu cấu trúc mô trong các mô được ủ với 2CA như được chỉ ra bởi các túi synap, mạch máu, mạng lưới tế bào thần kinh và dendrit, ty thể và màng trước synap còn nguyên vẹn. CA cũng phục hồi các mức nguyên tố bị thay đổi trong các mô não. Những kết quả này chỉ ra tiềm năng kích thích của CA đối với việc sử dụng glucose của não với các hoạt động bảo vệ thần kinh đồng thời."} +{"idx": 1870301, "en": "This stereudy aimed at performing a histological and morphometric evaluation of the urethra and penis of male rabbits. Seven male New Zealand White rabbits weighing 2.1-3 kg were used in the study. The whole urethra, from the urinary bladder to the external urethral orifice, was dissected from the rabbits, and the tissue was sliced into sections at an interval of 2 mm. The sections were stained with haematoxylin-eosin, Masson-Goldner trichrome stain, Van Gieson's stain and Movat-Russell modified pentachrome stain. A detailed evaluation of the morphology and morphometry was performed. The parameters assessed were the type and height of epithelium, thickness and composition of connective tissue, and thickness and structure of muscularis. The histological structure of the rabbit urethra was found to be similar to humans. However, although the rabbits were found to have the same type of penis as the humans, the internal structure of the corpora cavernosa, the relative thickness of the tunica albuginea and the rudimentary glands of the penis were found to differ in these animals. The results of the present study may be useful in the designing of implants, drug testing or surgical procedures on the physiological and pathological urethra.", "gemini": "Nghiên cứu này nhằm thực hiện đánh giá mô học và đo lường hình thái của niệu đạo và dương vật của thỏ đực. Bảy con thỏ trắng New Zealand đực nặng 2,1-3 kg được sử dụng trong nghiên cứu. Toàn bộ niệu đạo, từ bàng quang đến lỗ niệu đạo ngoài, được mổ xẻ từ những con thỏ và mô được cắt thành các phần với khoảng cách 2 mm. Các phần được nhuộm bằng hematoxylin-eosin, nhuộm Masson-Goldner trichrome, nhuộm Van Gieson và nhuộm pentachrome cải tiến Movat-Russell. Một đánh giá chi tiết về hình thái và phép đo hình thái đã được thực hiện. Các thông số được đánh giá là loại và chiều cao của biểu mô, độ dày và thành phần của mô liên kết, độ dày và cấu trúc của cơ. Cấu trúc mô học của niệu đạo thỏ được phát hiện là tương tự như ở người. Tuy nhiên, mặc dù những con thỏ được phát hiện có cùng loại dương vật với con người, nhưng cấu trúc bên trong của thể hang, độ dày tương đối của lớp áo trắng và các tuyến thô sơ của dương vật được phát hiện khác nhau ở những loài động vật này. Kết quả của nghiên cứu hiện tại có thể hữu ích trong việc thiết kế các phương pháp cấy ghép, thử thuốc hoặc các thủ thuật phẫu thuật trên niệu đạo sinh lý và bệnh lý."} +{"idx": 1870302, "en": "Collection of a blood sample defined by the term \"blood liquid biopsy\" is commonly used to detect diagnostic, prognostic, and therapeutic decision-making markers of metastatic tumors including circulating tumor cells (CTCs). Many tumors also release CTCs and other markers into lymph fluid, but the utility of lymphatic markers largely remains unexplored. Here, we introduce lymph liquid biopsy through collection of peripheral (afferent) and central (thoracic duct [TD]) lymph samples and demonstrates its feasibility for detection of stem-like CTCs potentially responsible for metastasis development and tumor relapse. Stemness of lymphatic CTCs (L-CTCs) was determined by spheroid-forming assay in vitro. Simultaneously, we tested blood CTCs by conventional blood liquid biopsy, and monitored the primary tumor size, early metastasis in a sentinel lymph node (SLN) and distant metastasis in lungs. Using a mouse model at early melanoma stage with no distant metastasis, we identified stem-like L-CTCs in lymph samples from afferent lymphatic vessels. Since these vessels transport cells from the primary tumor to SLN, our finding emphasizes the significance of the lymphatic pathway in development of SLN metastasis. Surprisingly, in pre-metastatic disease, stem-like L-CTCs were detected in lymph samples from the TD, which directly empties lymph into blood circulation. This suggests a new contribution of the lymphatic system to initiation of distant metastasis. Integration of lymph and blood liquid biopsies demonstrated that all mice with early melanoma had stem-like CTCs in at least one of three samples (afferent lymph, TD lymph, and blood). At the stage of distant metastasis, spheroid-forming L-CTCs were detected in TD lymph, but not in afferent lymph. Altogether, our results demonstrated that lymph liquid biopsy and testing L-CTCs holds promise for diagnosis and prognosis of early metastasis. © 2020 International Society for Advancement of Cytometry.", "gemini": "Việc thu thập mẫu máu được định nghĩa bằng thuật ngữ \"sinh thiết lỏng máu\" thường được sử dụng để phát hiện các dấu hiệu chẩn đoán, tiên lượng và ra quyết định điều trị của các khối u di căn bao gồm các tế bào khối u lưu hành (CTC). Nhiều khối u cũng giải phóng CTC và các dấu hiệu khác vào dịch bạch huyết, nhưng tiện ích của các dấu hiệu bạch huyết phần lớn vẫn chưa được khám phá. Tại đây, chúng tôi giới thiệu sinh thiết lỏng bạch huyết thông qua việc thu thập các mẫu bạch huyết ngoại vi (hướng tâm) và trung tâm (ống ngực [TD]) và chứng minh tính khả thi của nó đối với việc phát hiện các CTC giống tế bào gốc có khả năng chịu trách nhiệm cho sự phát triển di căn và tái phát khối u. Tính gốc của CTC bạch huyết (L-CTC) được xác định bằng xét nghiệm hình thành hình cầu in vitro. Đồng thời, chúng tôi đã thử nghiệm CTC máu bằng sinh thiết lỏng máu thông thường và theo dõi kích thước khối u nguyên phát, di căn sớm ở hạch bạch huyết tiền哨 (SLN) và di căn xa ở phổi. Sử dụng mô hình chuột ở giai đoạn u hắc tố sớm không có di căn xa, chúng tôi đã xác định được L-CTC giống tế bào gốc trong các mẫu bạch huyết từ các mạch bạch huyết hướng tâm. Vì các mạch này vận chuyển các tế bào từ khối u nguyên phát đến SLN, nên phát hiện của chúng tôi nhấn mạnh tầm quan trọng của đường bạch huyết trong sự phát triển của di căn SLN. Đáng ngạc nhiên là ở bệnh tiền di căn, L-CTC giống tế bào gốc đã được phát hiện trong các mẫu bạch huyết từ TD, nơi đổ trực tiếp bạch huyết vào tuần hoàn máu. Điều này cho thấy một đóng góp mới của hệ bạch huyết vào quá trình khởi phát di căn xa. Việc tích hợp sinh thiết lỏng bạch huyết và máu cho thấy tất cả những con chuột bị u hắc tố sớm đều có CTC giống tế bào gốc trong ít nhất một trong ba mẫu (bạch huyết hướng tâm, bạch huyết TD và máu). Ở giai đoạn di căn xa, L-CTC hình thành hình cầu đã được phát hiện trong bạch huyết TD, nhưng không có ở bạch huyết hướng tâm. Nhìn chung, kết quả của chúng tôi chứng minh rằng sinh thiết lỏng bạch huyết và thử nghiệm L-CTC có triển vọng trong chẩn đoán và tiên lượng di căn sớm. © 2020 Hiệp hội quốc tế về tiến bộ của tế bào học."} +{"idx": 1870303, "en": "OBJECTIVE A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability. METHODS A literature search was performed on the Advanced search field of PubMed using the keywords: \"(stability) AND (analytes OR laboratory analytes OR laboratory tests OR biochemical analytes OR biochemical tests OR biochemical laboratory tests).\" A total of 56 entries were obtained. After applying the selection criteria, 20 articles were included in the study. RESULTS In the 20 included references, up to 123 different analytes were assessed. The 34 analytes in order of the most frequently studied analytes were evaluated: Alanine aminotransferase, aspartate aminotransferase, potassium, triglyceride, alkaline phosphatase, creatinine, total cholesterol, albumin, lactate dehydrogenase, sodium, calcium, γ-glutamyltransferase, total bilirubin, urea, creatine kinase, inorganic phosphate, total protein, uric acid, amylase, chloride, high-density lipoprotein, magnesium, glucose, C-reactive protein, bicarbonate, ferritin, iron, lipase, transferrin, cobalamin, cortisol, folate, free thyroxine, and thyroid-stimulating hormone. Stable test results could be varied between 2 hours and 1 week according to the type of samples and/or type of blood collection tubes on a basic classification set as refrigerated or room temperature. CONCLUSIONS Biochemical analytes stability could be improved if the best pre-analytical approaches are used.", "gemini": "MỤC TIÊU Một vấn đề phổ biến trong các phòng xét nghiệm lâm sàng là duy trì sự ổn định của các chất phân tích trong quá trình tiền phân tích. Mục đích của nghiên cứu này là tóm tắt một cách có hệ thống các kết quả của một loạt các nghiên cứu về độ ổn định của các chất phân tích sinh hóa. PHƯƠNG PHÁP Đã thực hiện tìm kiếm tài liệu trên trường Tìm kiếm nâng cao của PubMed bằng cách sử dụng các từ khóa: \"(ổn định) VÀ (chất phân tích HOẶC chất phân tích trong phòng thí nghiệm HOẶC xét nghiệm trong phòng thí nghiệm HOẶC chất phân tích sinh hóa HOẶC xét nghiệm sinh hóa HOẶC xét nghiệm sinh hóa trong phòng thí nghiệm).\" Tổng cộng thu được 56 mục. Sau khi áp dụng các tiêu chí lựa chọn, 20 bài báo đã được đưa vào nghiên cứu. KẾT QUẢ Trong 20 tài liệu tham khảo được đưa vào, có tới 123 chất phân tích khác nhau được đánh giá. 34 chất phân tích được đánh giá theo thứ tự các chất phân tích được nghiên cứu thường xuyên nhất: Alanine aminotransferase, aspartate aminotransferase, kali, triglyceride, phosphatase kiềm, creatinine, cholesterol toàn phần, albumin, lactate dehydrogenase, natri, canxi, γ-glutamyltransferase, bilirubin toàn phần, urê, creatine kinase, vô cơ phosphate, protein toàn phần, axit uric, amylase, chloride, lipoprotein tỷ trọng cao, magiê, glucose, protein C phản ứng, bicarbonate, ferritin, sắt, lipase, transferrin, cobalamin, cortisol, folate, thyroxine tự do và hormone kích thích tuyến giáp. Kết quả xét nghiệm ổn định có thể thay đổi trong khoảng từ 2 giờ đến 1 tuần tùy theo loại mẫu và/hoặc loại ống lấy máu trên cơ sở phân loại cơ bản được đặt là nhiệt độ lạnh hoặc nhiệt độ phòng. KẾT LUẬN Độ ổn định của các chất phân tích sinh hóa có thể được cải thiện nếu sử dụng các phương pháp tiếp cận tiền phân tích tốt nhất."} +{"idx": 1870304, "en": "The plant Scutellaria barbata (SB) is commonly used as herbal medicines for treating cancer. The present pre-clinical study aimed to validate the Chinese Pharmacopoeia (CP) recommended dosages of SB water extract (SBW) in treating colon tumors. The content of chemical marker scutellarin in SBW was quantified using UPLC. Mice bearing human HCT116 xenografts or murine colon26 tumors received oral administration of SBW or scutellarin for 4 weeks. Results showed that SBW (615 and 1,230 mg/kg) and scutellarin (7 mg/kg) treatments significantly reduced human xenograft weights by 28.7, 36.9 and 28.8%, respectively. Lung metastasis area could be ameliorated after SBW (615 mg/kg) and scutellarin (7 mg/kg) treatments by 23.4 and 29.5%, respectively. Expressions of colon cancer metastasis-related proteins E-cadherin, Tspan 8 and CXCR4, as well as Src kinase in tumors were first shown to be regulated by SBW. Furthermore, in murine colon26 tumor-bearing mice, SBW (615 mg/kg) and scutellarin (7 mg/kg) treatments reduced the orthotopic tumor burden by 94.7% and lung metastatic tumor burden by 94.1%, respectively. Our findings provided evidences that SBW (at the mouse equivalent dosages to clinical dosages recommended by CP) could exert anti-tumor and anti-metastatic effects in colon cancer animal models.", "gemini": "Cây Scutellaria barbata (SB) thường được sử dụng làm thuốc thảo dược để điều trị ung thư. Nghiên cứu tiền lâm sàng hiện tại nhằm xác nhận liều lượng chiết xuất nước SB (SBW) theo khuyến cáo của Dược điển Trung Quốc (CP) trong điều trị khối u đại tràng. Hàm lượng chất đánh dấu hóa học scutellarin trong SBW được định lượng bằng UPLC. Chuột mang khối u xen ghép HCT116 ở người hoặc khối u colon26 ở chuột được uống SBW hoặc scutellarin trong 4 tuần. Kết quả cho thấy các phương pháp điều trị bằng SBW (615 và 1.230 mg/kg) và scutellarin (7 mg/kg) làm giảm đáng kể trọng lượng khối u xen ghép ở người lần lượt là 28,7, 36,9 và 28,8%. Diện tích di căn phổi có thể được cải thiện sau khi điều trị bằng SBW (615 mg/kg) và scutellarin (7 mg/kg) lần lượt là 23,4 và 29,5%. Các biểu hiện của protein liên quan đến di căn ung thư đại tràng E-cadherin, Tspan 8 và CXCR4, cũng như kinase Src trong khối u lần đầu tiên được chứng minh là được điều chỉnh bởi SBW. Hơn nữa, ở những con chuột mang khối u colon26 ở chuột, các phương pháp điều trị bằng SBW (615 mg/kg) và scutellarin (7 mg/kg) làm giảm gánh nặng khối u tại chỗ là 94,7% và gánh nặng khối u di căn phổi là 94,1%, tương ứng. Những phát hiện của chúng tôi cung cấp bằng chứng cho thấy SBW (ở liều tương đương ở chuột với liều lâm sàng được CP khuyến cáo) có thể phát huy tác dụng chống khối u và chống di căn ở các mô hình động vật ung thư đại tràng."} +{"idx": 1870305, "en": "BACKGROUND Youth mental health and well-being is of increasing concern to practitioners and policy-makers. Youth experiencing mental health problems face many barriers in accessing care and often have different needs to those of adults experiencing mental health problems. In order to adequately respond to the needs of young people aged 12-25, it is necessary to understand, from their perspective, their diverse needs and their different realities, using a global health approach and through participation in the development of mental health services and care. There are documented difficulties in implementing a recovery-oriented practice approach that have led to misapplications, misunderstandings and critiques. That said, there is little research or discussion on mental health recovery by and for young people and young adults. PURPOSE To help child and adolescent mental health practitioners better assess the pertinence of the mental health recovery model in their practice, a focus on the emergence of the model can be helpful in order to adapt the current conceptions of mental health recovery for work with youth. CONCLUSION Child and adolescent mental health professionals might want to consider the following three suggestions - consider developmental processes, focus on hope and create strong community ties.", "gemini": "HOÀN CẢNH Sức khỏe tâm thần và hạnh phúc của thanh thiếu niên đang là mối quan tâm ngày càng tăng đối với các học viên và nhà hoạch định chính sách. Thanh thiếu niên gặp vấn đề về sức khỏe tâm thần phải đối mặt với nhiều rào cản trong việc tiếp cận dịch vụ chăm sóc và thường có nhu cầu khác với những người trưởng thành gặp vấn đề về sức khỏe tâm thần. Để đáp ứng đầy đủ nhu cầu của những người trẻ tuổi từ 12-25 tuổi, cần phải hiểu, từ góc độ của họ, những nhu cầu đa dạng và thực tế khác nhau của họ, bằng cách sử dụng cách tiếp cận sức khỏe toàn cầu và thông qua việc tham gia vào quá trình phát triển các dịch vụ và chăm sóc sức khỏe tâm thần. Có những khó khăn được ghi nhận trong việc triển khai cách tiếp cận thực hành hướng phục hồi đã dẫn đến việc áp dụng sai, hiểu lầm và chỉ trích. Điều đó nói rằng, có rất ít nghiên cứu hoặc thảo luận về phục hồi sức khỏe tâm thần của và cho những người trẻ tuổi và thanh niên. MỤC ĐÍCH Để giúp các học viên sức khỏe tâm thần trẻ em và vị thành niên đánh giá tốt hơn tính phù hợp của mô hình phục hồi sức khỏe tâm thần trong thực hành của họ, việc tập trung vào sự xuất hiện của mô hình có thể hữu ích để điều chỉnh các quan niệm hiện tại về phục hồi sức khỏe tâm thần cho công tác với thanh thiếu niên. KẾT LUẬN Các chuyên gia sức khỏe tâm thần trẻ em và vị thành niên có thể muốn xem xét ba đề xuất sau - xem xét các quá trình phát triển, tập trung vào hy vọng và tạo ra mối liên hệ cộng đồng chặt chẽ."} +{"idx": 1870306, "en": "MR is a prominent technology to investigate diseases, with millions of clinical procedures performed every year. Metabolic dysfunction is one common aspect associated with many diseases. Thus, understanding and monitoring metabolic changes is essential to develop cures for many illnesses, including for example cancer and neurodegeneration. MR methodologies are especially suited to study endogenous metabolites and processes within an organism in vivo, which has led to many insights about physiological functions. Advancing metabolic MR techniques is therefore key to further understand physiological processes. Here, we introduce an approach based on nuclear spin singlet states to specifically filter metabolic signals and particularly show that singlet-filtered glutamate can be observed distinctly in the hippocampus of a living mouse in vivo. This development opens opportunities to make use of the singlet spin phenomenon in vivo and besides its use as a filter to provide scope for new contrast agents.", "gemini": "MR là một công nghệ nổi bật để nghiên cứu bệnh tật, với hàng triệu quy trình lâm sàng được thực hiện hàng năm. Rối loạn chuyển hóa là một khía cạnh phổ biến liên quan đến nhiều bệnh. Do đó, việc hiểu và theo dõi các thay đổi chuyển hóa là rất cần thiết để phát triển phương pháp chữa trị cho nhiều bệnh, bao gồm cả ung thư và thoái hóa thần kinh. Các phương pháp MR đặc biệt phù hợp để nghiên cứu các chất chuyển hóa nội sinh và các quá trình bên trong một cơ thể sống, điều này đã dẫn đến nhiều hiểu biết sâu sắc về các chức năng sinh lý. Do đó, việc thúc đẩy các kỹ thuật MR chuyển hóa là chìa khóa để hiểu sâu hơn về các quá trình sinh lý. Ở đây, chúng tôi giới thiệu một phương pháp dựa trên các trạng thái đơn của hạt nhân spin để lọc đặc hiệu các tín hiệu chuyển hóa và đặc biệt cho thấy rằng có thể quan sát thấy glutamate được lọc đơn một cách rõ ràng ở vùng đồi hải mã của một con chuột sống. Sự phát triển này mở ra cơ hội để sử dụng hiện tượng spin đơn trong cơ thể sống và bên cạnh việc sử dụng nó như một bộ lọc để cung cấp phạm vi cho các tác nhân tạo độ tương phản mới."} +{"idx": 1870307, "en": "Policy Points Well-being In the Nation (WIN) offers the first parsimonious set of vetted common measures to improve population health and social determinants across sectors at local, state, and national levels and is driven by what communities need to improve health, well-being, and equity. The WIN measures were codesigned with more than 100 communities, federal agencies, and national organizations across sectors, in alignment with the National Committee on Vital and Health Statistics, the Foundations for Evidence-Based Policymaking Act, and Healthy People 2030. WIN offers a process for a collaborative learning measurement system to drive a learning health and well-being system across sectors at the community, state, and national levels. The WIN development process identified critical gaps and opportunities in equitable community-level data infrastructure, interoperability, and protections that could be used to inform the Federal Data Strategy.", "gemini": "Các điểm chính sách Sức khỏe toàn dân (WIN) cung cấp bộ biện pháp chung đầu tiên được kiểm tra cẩn thận để cải thiện sức khỏe cộng đồng và các yếu tố xã hội quyết định sức khỏe trên nhiều lĩnh vực ở cấp địa phương, tiểu bang và quốc gia và được thúc đẩy bởi những gì cộng đồng cần để cải thiện sức khỏe, sức khỏe và công bằng. Các biện pháp WIN đã được thiết kế cùng với hơn 100 cộng đồng, cơ quan liên bang và các tổ chức quốc gia trên nhiều lĩnh vực, phù hợp với Ủy ban Thống kê Sức khỏe và Sức khỏe Toàn quốc, Đạo luật Xây dựng Chính sách Dựa trên Bằng chứng và Sức khỏe Toàn dân 2030. WIN cung cấp một quy trình cho hệ thống đo lường học tập hợp tác để thúc đẩy hệ thống sức khỏe và sức khỏe học tập trên nhiều lĩnh vực ở cấp cộng đồng, tiểu bang và quốc gia. Quá trình phát triển WIN đã xác định được những khoảng cách và cơ hội quan trọng trong cơ sở hạ tầng dữ liệu cấp cộng đồng công bằng, khả năng tương tác và các biện pháp bảo vệ có thể được sử dụng để thông báo cho Chiến lược Dữ liệu Liên bang."} +{"idx": 1870308, "en": "The transition-metal-free cross-coupling of alkyl or aryl electrophiles by using tertiary benzylic organoboronates is reported. This reaction involves the generation of tertiary alkyl anions from organoboronates in the presence of an alkoxide base and then their substitution reactions. This protocol allows the simple and efficient construction of quaternary carbon centers.", "gemini": "Phản ứng ghép chéo không có kim loại chuyển tiếp của các chất ái điện tử alkyl hoặc aryl bằng cách sử dụng organoborat benzylic bậc ba đã được báo cáo. Phản ứng này liên quan đến sự tạo thành các anion alkyl bậc ba từ organoborat khi có mặt của bazơ alkoxide và sau đó là phản ứng thế của chúng. Giao thức này cho phép xây dựng đơn giản và hiệu quả các trung tâm cacbon bậc bốn."} +{"idx": 1870309, "en": "The main challenge of ensiling is conserving the feed through a fermentative process that results in high nutritional and microbiological quality while minimizing fermentative losses. This challenge is of growing interest to farmers, industry and research and involves the use of additives to improve the fermentation process and preserve the ensiled material. Most studies involved microbial additives; lactic acid bacteria (LAB) have been the focus of much research and have been widely used. Currently, LABs are used in modern and sustainable agriculture because of their considerable potential for enhancing human and animal health. Although the number of studies evaluating LABs in silages has increased, the potential use of these micro-organisms in association with silage has not been adequately studied. Fermentation processes using the same strain produce very different results depending on the unique characteristics of the substrate, so the choice of silage inoculant for different starting substrates is of extreme importance to maximize the nutritional quality of the final product. This review describes the current scenario of the bioprospecting and selection process for choosing the best LAB strain as an inoculant for ensiling. In addition, we analyse developments in the fermentation process and strategies and methods that will assist future studies on the selection of new strains of LAB as a starter culture or inoculant.", "gemini": "Thách thức chính của quá trình ủ chua là bảo quản thức ăn thông qua quá trình lên men, tạo ra chất lượng dinh dưỡng và vi sinh cao trong khi giảm thiểu tổn thất lên men. Thách thức này ngày càng được các nhà nông, ngành công nghiệp và nghiên cứu quan tâm và liên quan đến việc sử dụng các chất phụ gia để cải thiện quá trình lên men và bảo quản vật liệu ủ chua. Hầu hết các nghiên cứu liên quan đến các chất phụ gia vi sinh; vi khuẩn axit lactic (LAB) đã trở thành trọng tâm của nhiều nghiên cứu và được sử dụng rộng rãi. Hiện nay, LAB được sử dụng trong nông nghiệp hiện đại và bền vững vì tiềm năng đáng kể của chúng trong việc nâng cao sức khỏe của con người và động vật. Mặc dù số lượng các nghiên cứu đánh giá LAB trong thức ăn ủ chua đã tăng lên, nhưng việc sử dụng tiềm năng của các vi sinh vật này kết hợp với thức ăn ủ chua vẫn chưa được nghiên cứu đầy đủ. Các quá trình lên men sử dụng cùng một chủng tạo ra kết quả rất khác nhau tùy thuộc vào các đặc điểm riêng biệt của giá thể, vì vậy việc lựa chọn chất cấy ủ chua cho các giá thể ban đầu khác nhau là cực kỳ quan trọng để tối đa hóa chất lượng dinh dưỡng của sản phẩm cuối cùng. Bài đánh giá này mô tả tình hình hiện tại của quá trình tìm kiếm sinh học và lựa chọn để chọn chủng LAB tốt nhất làm chất cấy cho quá trình ủ chua. Ngoài ra, chúng tôi phân tích các phát triển trong quá trình lên men và các chiến lược và phương pháp sẽ hỗ trợ các nghiên cứu trong tương lai về việc lựa chọn các chủng LAB mới làm nền văn hóa khởi đầu hoặc chất cấy."} +{"idx": 1870310, "en": "In assessing biosimilarity between two products, the question to ask is always \"How similar is similar?\" Traditionally, the equivalence of the means between products is the primary consideration in a clinical trial. This study suggests an alternative assessment for testing a certain percentage of the population of differences lying within a prespecified interval. In doing so, the accuracy and precision are assessed simultaneously by judging whether a two-sided tolerance interval falls within a prespecified acceptance range. We further derive an asymptotic distribution of the tolerance limits to determine the sample size for achieving a targeted level of power. Our numerical study shows that the proposed two-sided tolerance interval test controls the type I error rate and provides sufficient power. A real example is presented to illustrate our proposed approach.", "gemini": "Trong quá trình đánh giá tính tương đồng sinh học giữa hai sản phẩm, câu hỏi luôn được đặt ra là \"Tương tự đến mức nào là tương tự?\" Theo truyền thống, sự tương đương về phương tiện giữa các sản phẩm là yếu tố chính được xem xét trong thử nghiệm lâm sàng. Nghiên cứu này đề xuất một đánh giá thay thế để kiểm tra một tỷ lệ phần trăm nhất định của quần thể về sự khác biệt nằm trong một khoảng được chỉ định trước. Khi làm như vậy, độ chính xác và độ chính xác được đánh giá đồng thời bằng cách đánh giá xem khoảng dung sai hai chiều có nằm trong phạm vi chấp nhận được chỉ định trước hay không. Hơn nữa, chúng tôi còn tìm ra phân phối tiệm cận của giới hạn dung sai để xác định quy mô mẫu để đạt được mức công suất mục tiêu. Nghiên cứu số của chúng tôi cho thấy thử nghiệm khoảng dung sai hai chiều được đề xuất kiểm soát tỷ lệ lỗi loại I và cung cấp đủ công suất. Một ví dụ thực tế được trình bày để minh họa cách tiếp cận được đề xuất của chúng tôi."} +{"idx": 1870311, "en": "Prognosis of hepatitis B (HBV)-associated hepatocellular carcinoma (HCC) is poor due to high rates of HCC recurrence and progression of underlying liver disease. We studied whether serum hepatitis B core-related antigen (HBcrAg) levels could predict HCC recurrence and outcome in HBV associated. Higher HBcrAg levels at HCC diagnosis were independently associated with reduced overall and recurrence-free survival in patients with early, but not advanced, stage HCC.", "gemini": "Tiên lượng của ung thư biểu mô tế bào gan (HCC) liên quan đến viêm gan B (HBV) là kém do tỷ lệ tái phát HCC cao và tiến triển của bệnh gan tiềm ẩn. Chúng tôi đã nghiên cứu xem nồng độ kháng nguyên liên quan đến nhân viêm gan B (HBcrAg) trong huyết thanh có thể dự đoán được sự tái phát và kết cục của HCC liên quan đến HBV hay không. Nồng độ HBcrAg cao hơn khi chẩn đoán HCC có liên quan độc lập với tỷ lệ sống sót toàn thể và không tái phát ở những bệnh nhân mắc HCC giai đoạn sớm, nhưng không phải giai đoạn tiến triển."} +{"idx": 1870312, "en": "Selaginella moellendorffii Hieron. (SM), a perennial evergreen plant, has been used in the treatment of acute infectious hepatitis, thoracic and hypochondriac lumbar contusions, systemic oedema and thrombocytopaenia. However, the role of a biflavonoid-rich extract from SM (SM-BFRE) in anti-larynx cancer has rarely been reported. In this study, the in vitro and in vivo anti-laryngeal cancer activity and potential mechanisms of SM-BFRE were investigated. An off-line semipreparative liquid chromatography-nuclear magnetic resonance protocol was carried out to determine six biflavonoids from SM-BFRE. In vitro, MTT assay revealed that SM-BFRE inhibited the proliferation of laryngeal carcinoma cells. A wound healing assay indicated that SM-BFRE suppressed the migration of laryngeal cancer cells. Hoechst 33 258 and Annexin V-FITC/PI double staining assays were performed and verified that SM-BFRE induced apoptosis in laryngeal carcinoma cells. The Hep-2 bearing nude mouse model confirmed that SM-BFRE also exhibited anticancer effect in vivo. In addition, Western blot analysis demonstrated that SM-BFRE exerted its anti-laryngeal cancer effect by activating the mitochondrial apoptotic pathway and inhibiting STAT3 and Akt/NF-κB signalling pathways. All results suggested that SM-BFRE could be considered as a potential chemotherapeutic drug for laryngeal cancer.", "gemini": "Selaginella moellendorffii Hieron. (SM), một loại cây thường xanh lâu năm, đã được sử dụng trong điều trị viêm gan truyền nhiễm cấp tính, vết bầm ngực và thắt lưng hạ sườn, phù toàn thân và giảm tiểu cầu. Tuy nhiên, vai trò của chiết xuất giàu biflavonoid từ SM (SM-BFRE) trong chống ung thư thanh quản hiếm khi được báo cáo. Trong nghiên cứu này, hoạt tính chống ung thư thanh quản trong ống nghiệm và trong cơ thể sống và các cơ chế tiềm ẩn của SM-BFRE đã được nghiên cứu. Một giao thức sắc ký lỏng bán chuẩn bị ngoại tuyến - cộng hưởng từ hạt nhân đã được thực hiện để xác định sáu biflavonoid từ SM-BFRE. Trong ống nghiệm, xét nghiệm MTT cho thấy SM-BFRE ức chế sự gia tăng của các tế bào ung thư thanh quản. Xét nghiệm lành vết thương chỉ ra rằng SM-BFRE ức chế sự di chuyển của các tế bào ung thư thanh quản. Các xét nghiệm nhuộm kép Hoechst 33 258 và Annexin V-FITC/PI đã được thực hiện và xác minh rằng SM-BFRE gây ra apoptosis ở các tế bào ung thư thanh quản. Mô hình chuột nude mang Hep-2 xác nhận rằng SM-BFRE cũng biểu hiện tác dụng chống ung thư trong cơ thể sống. Ngoài ra, phân tích Western blot chứng minh rằng SM-BFRE phát huy tác dụng chống ung thư thanh quản của mình bằng cách kích hoạt con đường apoptosis ty thể và ức chế các con đường truyền tín hiệu STAT3 và Akt/NF-κB. Tất cả các kết quả đều gợi ý rằng SM-BFRE có thể được coi là một loại thuốc hóa trị tiềm năng cho bệnh ung thư thanh quản."} +{"idx": 1870313, "en": "As patients with chronic hepatitis C virus (HCV) tend to be older and/or have advanced liver disease in Japan, real-world data are needed to evaluate safe and effective treatment options. The study aim was to assess safety and effectiveness of ledipasvir/sofosbuvir (LDV/SOF) in a real-world cohort of Japanese patients with HCV genotype (GT) 1 infection overall and by patient subgroups: elderly, compensated cirrhotic, advanced fibrotic and those with hepatocellular carcinoma (HCC). A large prospective observational study was conducted, enrolling adult patients treated for HCV GT1 infection with LDV/SOF at clinical sites across Japan. Patients were observed for safety outcomes during and 4 weeks after treatment, and for sustained virologic response at 12-weeks post-treatment (SVR12). Incidence rates (IRs) of adverse drug reactions (ADRs) and serious ADRs (SADRs) and SVR12 rates were assessed overall and by subgroups. ADR and SADR IRs were low (2.26 and 0.17 per 100 person-months, respectively) and did not significantly differ in elderly patients or those with presence of compensated cirrhosis, worsening fibrosis or HCC. SVR12 rates were high overall (98.5%) and across subgroups investigated (≥94%), including patients who were elderly (98.2%), treatment-experienced (97.6%), advanced fibrotic (≥95.8%), had existing NS5A resistance-associated substitutions reported pre-treatment (95.0%), compensated cirrhosis (95.7%), HCC (94.0%) and other chronic liver diseases (96.1%). In this large, real-world observational study of Japanese patients with HCV GT1 infection, LDV/SOF treatment resulted in low incidence of adverse events, with high real-world effectiveness, even among patients with potentially higher risks of adverse safety outcomes and treatment failure.", "gemini": "Vì bệnh nhân bị viêm gan C mạn tính (HCV) có xu hướng lớn tuổi hơn và/hoặc mắc bệnh gan tiến triển ở Nhật Bản, nên cần có dữ liệu thực tế để đánh giá các lựa chọn điều trị an toàn và hiệu quả. Mục đích của nghiên cứu là đánh giá độ an toàn và hiệu quả của ledipasvir/sofosbuvir (LDV/SOF) trong một nhóm bệnh nhân người Nhật bị nhiễm HCV kiểu gen (GT) 1 nói chung và theo các nhóm bệnh nhân: người cao tuổi, xơ gan được bù, xơ hóa tiến triển và những người mắc ung thư biểu mô tế bào gan (HCC). Một nghiên cứu quan sát triển vọng lớn đã được tiến hành, tuyển dụng những bệnh nhân trưởng thành được điều trị nhiễm HCV GT1 bằng LDV/SOF tại các cơ sở lâm sàng trên khắp Nhật Bản. Bệnh nhân được theo dõi về kết quả an toàn trong và 4 tuần sau khi điều trị, và về đáp ứng vi rút kéo dài ở thời điểm 12 tuần sau điều trị (SVR12). Tỷ lệ mắc (IR) của các phản ứng có hại của thuốc (ADR) và ADR nghiêm trọng (SADR) và tỷ lệ SVR12 đã được đánh giá tổng thể và theo các nhóm. IR của ADR và SADR thấp (lần lượt là 2,26 và 0,17 trên 100 người-tháng) và không khác biệt đáng kể ở bệnh nhân cao tuổi hoặc những bệnh nhân có xơ gan được bù, xơ hóa nặng hơn hoặc HCC. Tỷ lệ SVR12 cao trên toàn thể (98,5%) và giữa các nhóm được nghiên cứu (≥94%), bao gồm những bệnh nhân cao tuổi (98,2%), đã được điều trị (97,6%), xơ hóa tiến triển (≥95,8%), có các thay thế liên quan đến kháng NS5A hiện có được báo cáo trước khi điều trị (95,0%), xơ gan được bù (95,7%), HCC (94,0%) và các bệnh gan mạn tính khác (96,1%). Trong nghiên cứu quan sát thực tế lớn này ở những bệnh nhân người Nhật bị nhiễm HCV GT1, điều trị bằng LDV/SOF dẫn đến tỷ lệ mắc các tác dụng phụ thấp, với hiệu quả thực tế cao, ngay cả ở những bệnh nhân có nguy cơ cao hơn về các kết cục an toàn bất lợi và điều trị thất bại."} +{"idx": 1870314, "en": "Human epidermal growth factor receptor 2 (HER2) protein overexpression is associated with HER2 gene amplification, a critical driver oncogenetic change in gastric cancer. HER2 heterogeneity in advanced gastric cancer is associated with a poor prognosis and affects the clinical efficacy of trastuzumab. However, the mechanisms of HER2 heterogeneity are not fully understood. Here, we examined whether HER2 heterogeneous gastric cancer exhibited intratumoral genetic heterogeneity in other cancer-related genes. Two cases of advanced gastric cancer with HER2 heterogeneity were selected, and samples of HER2-positive and HER2-negative areas in each case were analyzed using a cancer-associated multiple gene panel. In both cases, TP53 mutations were observed in both HER2-positive and HER2-negative areas, whereas many of the potential driver and passenger mutations differed between HER2-positive and HER2-negative areas. Overall, our findings demonstrated that HER2 heterogeneous gastric cancer exhibited intratumoral genetic heterogeneity in other cancer-related genes and that the molecular mechanisms could differ between HER2-positive and -negative areas.", "gemini": "Sự biểu hiện quá mức của protein thụ thể yếu tố tăng trưởng biểu bì người 2 (HER2) có liên quan đến sự khuếch đại gen HER2, một thay đổi gây ung thư quan trọng trong ung thư dạ dày. Sự không đồng nhất của HER2 trong ung thư dạ dày tiến triển có liên quan đến tiên lượng xấu và ảnh hưởng đến hiệu quả lâm sàng của trastuzumab. Tuy nhiên, các cơ chế gây ra sự không đồng nhất của HER2 vẫn chưa được hiểu đầy đủ. Ở đây, chúng tôi kiểm tra xem ung thư dạ dày không đồng nhất HER2 có biểu hiện tính không đồng nhất di truyền trong khối u ở các gen liên quan đến ung thư khác hay không. Hai trường hợp ung thư dạ dày tiến triển có tính không đồng nhất HER2 đã được chọn và các mẫu ở các vùng dương tính với HER2 và âm tính với HER2 trong mỗi trường hợp đã được phân tích bằng một bảng gen đa liên quan đến ung thư. Trong cả hai trường hợp, đột biến TP53 đều được quan sát thấy ở cả vùng dương tính với HER2 và vùng âm tính với HER2, trong khi nhiều đột biến động lực và đột biến hành khách tiềm ẩn khác nhau giữa vùng dương tính với HER2 và vùng âm tính với HER2. Nhìn chung, những phát hiện của chúng tôi chứng minh rằng ung thư dạ dày không đồng nhất HER2 biểu hiện tính không đồng nhất di truyền trong khối u ở các gen liên quan đến ung thư khác và các cơ chế phân tử có thể khác nhau giữa các vùng dương tính và âm tính với HER2."} +{"idx": 1870315, "en": "Antibiotic resistance poses a great threat to human, animal and environmental health. β-Lactam antibiotics have been successful in combating bacterial infections. However, the overuse, inappropriate prescribing, unavailability of new antibiotics and regulation barriers have exacerbated bacterial resistance to these antibiotics. 1,4,7-Triazacyclononane (TACN) is a cyclic organic tridentate inhibitor with strong metal-chelating abilities that has been shown to inhibit β-lactamase enzymes and may represent an important breakthrough in the treatment of drug-resistant bacterial strains. However, its cytotoxicity in the liver is unknown. This study aimed to determine the effect of TACN on oxidative stress in HepG2 cells. The HepG2 cells were treated with 0 to 500 µM TACN for 24 hours to obtain an IC50 for use in subsequent assays. Free radicals were measured using the thiobarbituric acid reactive substance and nitric oxide synthase assays, respectively, while antioxidant levels were assessed using luminometry (glutathione [GSH] and adenosine triphosphate [ATP]) and Western blot analysis (SOD, catalase, GPx-1, HSP70 and Nrf2). Percentage survival fluctuated as TACN concentration increased with a calculated IC50 of 545 µM. A slight increase in HSP70 and Nrf2 expression indicated the presence of stress and a response against it, respectively. However, free radical production was not increased as indicated by decreased malondialdehyde levels and reactive nitrogen species. Glutathione levels increased slightly, while ATP levels were marginally altered. The results suggest that TACN does not induce oxidative stress in HepG2 cells and can be exploited as a potential inhibitor.", "gemini": "Kháng thuốc kháng sinh gây ra mối đe dọa lớn đối với sức khỏe của con người, động vật và môi trường. Kháng sinh β-Lactam đã thành công trong việc chống lại các bệnh nhiễm trùng do vi khuẩn. Tuy nhiên, việc sử dụng quá mức, kê đơn không hợp lý, không có sẵn các loại kháng sinh mới và các rào cản về quy định đã làm trầm trọng thêm tình trạng kháng thuốc của vi khuẩn đối với các loại kháng sinh này. 1,4,7-Triazacyclononane (TACN) là chất ức chế ba răng hữu cơ vòng có khả năng tạo phức kim loại mạnh đã được chứng minh là có khả năng ức chế enzyme β-lactamase và có thể đại diện cho một bước đột phá quan trọng trong điều trị các chủng vi khuẩn kháng thuốc. Tuy nhiên, độc tính tế bào của nó trong gan vẫn chưa được biết rõ. Nghiên cứu này nhằm xác định tác dụng của TACN đối với stress oxy hóa trong tế bào HepG2. Các tế bào HepG2 được xử lý bằng TACN 0 đến 500 µM trong 24 giờ để có được IC50 để sử dụng trong các xét nghiệm tiếp theo. Các gốc tự do được đo bằng chất phản ứng với axit thiobarbituric và xét nghiệm nitric oxide synthase, trong khi đó, nồng độ chất chống oxy hóa được đánh giá bằng phương pháp đo độ sáng (glutathione [GSH] và adenosine triphosphate [ATP]) và phân tích Western blot (SOD, catalase, GPx-1, HSP70 và Nrf2). Tỷ lệ sống sót dao động khi nồng độ TACN tăng với IC50 được tính là 545 µM. Sự gia tăng nhẹ trong biểu hiện HSP70 và Nrf2 cho thấy sự hiện diện của stress và phản ứng chống lại nó. Tuy nhiên, sản xuất gốc tự do không tăng như được chỉ ra bởi mức malondialdehyde giảm và các loài nitơ phản ứng. Nồng độ glutathione tăng nhẹ, trong khi nồng độ ATP thay đổi không đáng kể. Kết quả cho thấy TACN không gây stress oxy hóa trong tế bào HepG2 và có thể được khai thác như một chất ức chế tiềm năng."} +{"idx": 1870316, "en": "Our memories are made of detailed sensory information representing the puzzle pieces of our personal past. The type of memory integrating sensory features is referred to as relational memory. The main objective of this study was to investigate whether relational memory is affected in children with autism spectrum disorder (ASD) since altered relational memory may contribute to atypical episodic memory observed in ASD. We also examined the association between perceptual style and relational memory abilities. Children with ASD (n = 14) and typically developed (TD) children (n = 16, 9-15 years old) completed a memory task with three conditions: two single-feature conditions measuring memory for objects and locations, and one relational memory condition measuring memory for objects and their locations combined. The Children's embedded figures test was administered to measure perceptual style. The ASD group selected more incorrect stimuli (false alarms) than the TD group, resulting in a lower proportion of correctly recognized targets across all memory conditions. The ASD group did not display a more local perceptual style than the TD group. However, perceptual style was associated with improved memory abilities across conditions. Our findings indicate that the overall memory performance of children with ASD is less stable, leading them to more incorrect responses than TD children. This may be due to the executive demands of the memory tasks, rather than specific impairments in memory binding. Autism Res 2020, 13: 1959-1969. © 2020 International Society for Autism Research and Wiley Periodicals LLC LAY SUMMARY: The present study shows that children with autism have a less stable memory than typically developed children, which is reflected in a higher amount of incorrect memory responses. Overall, our results indicate that children with autism display difficulties in differentiating previously studied from novel information when solving both single-feature memory tasks and a relational memory task (requiring memory of combination of features). These difficulties may have implications for how children with autism remember episodes from their personal past.", "gemini": "Ký ức của chúng ta được tạo nên từ thông tin chi tiết về giác quan đại diện cho những mảnh ghép của quá khứ cá nhân. Loại ký ức tích hợp các đặc điểm giác quan được gọi là ký ức quan hệ. Mục tiêu chính của nghiên cứu này là điều tra xem ký ức quan hệ có bị ảnh hưởng ở trẻ em mắc chứng rối loạn phổ tự kỷ (ASD) hay không vì ký ức quan hệ bị thay đổi có thể góp phần vào ký ức theo từng giai đoạn không điển hình được quan sát thấy ở ASD. Chúng tôi cũng đã kiểm tra mối liên quan giữa phong cách nhận thức và khả năng ghi nhớ quan hệ. Trẻ em mắc ASD (n = 14) và trẻ em phát triển bình thường (TD) (n = 16, 9-15 tuổi) đã hoàn thành một nhiệm vụ ghi nhớ với ba điều kiện: hai điều kiện đặc điểm đơn lẻ để đo lường trí nhớ đối với các đối tượng và địa điểm và một điều kiện trí nhớ quan hệ để đo lường trí nhớ đối với các đối tượng và địa điểm của chúng kết hợp. Bài kiểm tra hình ảnh nhúng của trẻ em được tiến hành để đo lường phong cách nhận thức. Nhóm ASD đã chọn nhiều kích thích không chính xác (báo động giả) hơn nhóm TD, dẫn đến tỷ lệ mục tiêu được nhận dạng chính xác thấp hơn trong tất cả các điều kiện trí nhớ. Nhóm ASD không biểu hiện phong cách nhận thức cục bộ hơn nhóm TD. Tuy nhiên, phong cách nhận thức có liên quan đến khả năng ghi nhớ được cải thiện trong các điều kiện. Những phát hiện của chúng tôi chỉ ra rằng hiệu suất ghi nhớ chung của trẻ em mắc ASD kém ổn định hơn, khiến chúng có nhiều phản ứng không chính xác hơn trẻ em TD. Điều này có thể là do nhu cầu điều hành của các nhiệm vụ ghi nhớ, chứ không phải do suy giảm cụ thể trong việc liên kết bộ nhớ. Autism Res 2020, 13: 1959-1969. © 2020 Hiệp hội nghiên cứu tự kỷ quốc tế và Wiley Periodicals LLC TÓM TẮT NGHIÊN CỨU: Nghiên cứu hiện tại cho thấy trẻ em mắc chứng tự kỷ có trí nhớ kém ổn định hơn so với trẻ em phát triển bình thường, điều này được phản ánh ở số lượng phản ứng ghi nhớ không chính xác cao hơn. Nhìn chung, kết quả của chúng tôi chỉ ra rằng trẻ em mắc chứng tự kỷ gặp khó khăn trong việc phân biệt thông tin đã học trước đó với thông tin mới khi giải quyết cả các nhiệm vụ ghi nhớ đặc điểm đơn lẻ và nhiệm vụ ghi nhớ quan hệ (yêu cầu ghi nhớ sự kết hợp của các đặc điểm). Những khó khăn này có thể có ý nghĩa đối với cách trẻ em mắc chứng tự kỷ ghi nhớ các tập phim trong quá khứ cá nhân của chúng."} +{"idx": 1870317, "en": "Psoriasis is a chronic, recurrent skin disease requiring long-term management. Agents that repair the skin's barrier function are invaluable additives in topical treatments of psoriasis. This multicenter, randomized, controlled trial evaluated the efficacy and safety of a linoleic acid-ceramide-containing moisturizer (LA-Cer) for mild-to-moderate psoriasis vulgaris. We randomized 178 patients from both northern and southern regions of China into two groups: 81 patients in the control group received mometasone furoate (MF, 0.1%) cream, while MF in combination with LA-Cer was administered to 86 patients in the treatment group for 4 weeks. The LA-Cer-MF group maintained the use of moisturizer after topical glucocorticoid administration. The primary endpoint, Psoriasis Area and Severity Index 50 (PASI 50) response, revealed the superiority of LA-Cer-MF with lower relapse rates at week 8. The use of the LA-Cer-containing moisturizer as maintenance therapy resulted in a continuous improvement in the clinical state in terms of body surface area, PASI, investigators' assessment of skin dryness and desquamation, and Physician Global Assessment of Psoriasis score, and in the patients' quality of life. Thus, the LA-Cer-containing moisturizer is a promising agent to prevent and treat psoriasis as it enhances the therapeutic effect induced by topical glucocorticoids and delays relapse.", "gemini": "Bệnh vẩy nến là một bệnh ngoài da mạn tính, tái phát cần được điều trị lâu dài. Các tác nhân giúp phục hồi chức năng hàng rào bảo vệ da là những chất bổ sung vô giá trong điều trị tại chỗ bệnh vẩy nến. Thử nghiệm có đối chứng, ngẫu nhiên, đa trung tâm này đã đánh giá hiệu quả và tính an toàn của một loại kem dưỡng ẩm chứa ceramide axit linoleic (LA-Cer) đối với bệnh vẩy nến thông thường từ nhẹ đến trung bình. Chúng tôi đã phân ngẫu nhiên 178 bệnh nhân từ cả miền bắc và miền nam Trung Quốc thành hai nhóm: 81 bệnh nhân trong nhóm đối chứng được dùng kem mometasone furoate (MF, 0,1%), trong khi MF kết hợp với LA-Cer được dùng cho 86 bệnh nhân trong nhóm điều trị trong 4 tuần. Nhóm LA-Cer-MF duy trì sử dụng kem dưỡng ẩm sau khi dùng glucocorticoid tại chỗ. Điểm cuối chính, đáp ứng Chỉ số diện tích và mức độ nghiêm trọng của bệnh vẩy nến 50 (PASI 50), cho thấy tính ưu việt của LA-Cer-MF với tỷ lệ tái phát thấp hơn vào tuần thứ 8. Việc sử dụng kem dưỡng ẩm chứa LA-Cer như một liệu pháp duy trì đã dẫn đến sự cải thiện liên tục về tình trạng lâm sàng về diện tích bề mặt cơ thể, PASI, đánh giá của bác sĩ điều tra về tình trạng khô và bong tróc da và điểm Đánh giá toàn cầu về bệnh vẩy nến của bác sĩ và chất lượng cuộc sống của bệnh nhân. Do đó, kem dưỡng ẩm chứa LA-Cer là một tác nhân đầy hứa hẹn để ngăn ngừa và điều trị bệnh vẩy nến vì nó tăng cường hiệu quả điều trị do glucocorticoid tại chỗ gây ra và làm chậm quá trình tái phát."} +{"idx": 1870318, "en": "Live vaccines are gradually replaced by protein-based vaccines given the latter's better safety. But live vaccines seem to be more efficient via stimulation of the innate immune system.", "gemini": "Vắc-xin sống đang dần được thay thế bằng vắc-xin gốc protein do độ an toàn tốt hơn. Nhưng vắc-xin sống có vẻ hiệu quả hơn thông qua việc kích thích hệ miễn dịch bẩm sinh."} +{"idx": 1870319, "en": "Mid- to high-latitude vegetation are experiencing changes in their seasonal cycles as a result of climate change. Although the rates of seasonal growth from winter dormancy to summer maturity have accelerated because of changes in environmental conditions, less attention has been paid to the rate of vegetation green-up (RVG) and its dynamics, which could advance vegetation maturity. We analyzed the long-term changes in RVG and the drivers at high northern latitudes for 35 years (1982-2016) using satellite-retrieved leaf area index data based on partial correlation analyses and multivariable linear regression. The rates tended to increase significantly with time, particularly at high latitudes above 60°N in North America (1.8% mon-1 decade-1 , p < .01) and Eurasia (1.0% mon-1 decade-1 , p < .01). The increasing trend in North America was mostly because of increased heat accumulation in spring (1.2% mon-1 decade-1 ), that is, more rapid green-up owing to warming, with an increased carbon dioxide concentration (0.6 mon-1 decade-1 ). The trend in Eurasia, however, was induced by warming, increased carbon dioxide concentration, and stronger radiation, 1.0%, 0.7%, and 0.5% mon-1 decade-1 , respectively, but was partly counteracted by earlier pregreen-up dates of -1.2% mon-1 decade-1 , that is, earlier initiation of growth which counteracted green-up rate acceleration. The results suggested that warming was the predominant factor influencing the accelerated RVG at high latitudes; however, Eurasian vegetation exhibited different green-up dynamics, mitigating the influence of warming with the earlier pregreen-up. Our findings imply that high-latitude warming will drive vegetation seasonality toward rapid green-up and early maturity, leading to the reinforcement of climate-vegetation interactions; however, the consequences will be more distinct in North America owing to the absence of alleviation by earlier pregreen-up.", "gemini": "Thảm thực vật ở vĩ độ trung bình đến cao đang trải qua những thay đổi trong chu kỳ theo mùa do biến đổi khí hậu. Mặc dù tốc độ tăng trưởng theo mùa từ trạng thái ngủ đông mùa đông đến trưởng thành mùa hè đã tăng tốc do những thay đổi về điều kiện môi trường, nhưng ít chú ý hơn đến tốc độ xanh hóa của thảm thực vật (RVG) và động lực của nó, có thể thúc đẩy sự trưởng thành của thảm thực vật. Chúng tôi đã phân tích những thay đổi lâu dài về RVG và các yếu tố thúc đẩy ở các vĩ độ phía bắc cao trong 35 năm (1982-2016) bằng cách sử dụng dữ liệu chỉ số diện tích lá thu được từ vệ tinh dựa trên phân tích tương quan từng phần và hồi quy tuyến tính đa biến. Tốc độ có xu hướng tăng đáng kể theo thời gian, đặc biệt là ở các vĩ độ cao trên 60°B ở Bắc Mỹ (1,8% mon-1 thập kỷ-1, p <0,01) và Âu Á (1,0% mon-1 thập kỷ-1, p <0,01). Xu hướng gia tăng ở Bắc Mỹ chủ yếu là do sự tích tụ nhiệt tăng vào mùa xuân (1,2% mon-1 thập kỷ-1), tức là xanh hóa nhanh hơn do ấm lên, với nồng độ carbon dioxide tăng (0,6 mon-1 thập kỷ-1). Tuy nhiên, xu hướng ở Âu Á lại do ấm lên, nồng độ carbon dioxide tăng và bức xạ mạnh hơn, lần lượt là 1,0%, 0,7% và 0,5% mon-1 thập kỷ-1, nhưng một phần bị phản tác dụng bởi ngày xanh hóa sớm hơn -1,2% mon-1 thập kỷ-1, tức là, sự khởi đầu tăng trưởng sớm hơn đã chống lại sự tăng tốc độ xanh hóa. Kết quả cho thấy rằng sự ấm lên là yếu tố chính ảnh hưởng đến RVG tăng tốc ở các vĩ độ cao; tuy nhiên, thảm thực vật Âu Á thể hiện động lực xanh hóa khác nhau, làm giảm ảnh hưởng của sự ấm lên với quá trình xanh hóa sớm hơn. Những phát hiện của chúng tôi ngụ ý rằng sự ấm lên ở vĩ độ cao sẽ thúc đẩy tính theo mùa của thảm thực vật theo hướng xanh hóa nhanh và trưởng thành sớm, dẫn đến sự củng cố tương tác khí hậu-thảm thực vật; tuy nhiên, hậu quả sẽ rõ rệt hơn ở Bắc Mỹ do không có sự giảm nhẹ do xanh hóa sớm hơn."} +{"idx": 1870320, "en": "Accumulating studies authenticate that circular RNAs (circRNAs) are involved in the progression of cancers. However, their role in breast cancer (BC) remains largely unknown. In this study, real-time polymerase chain reaction was employed to detect the circ_0000291 and miR-326 expressions in BC tissues and cells. The correlation between the expression level of circ_0000291 and clinicopathological parameters of BC patients was analyzed. Western blot was used to detect the expression of E26 transformation-specific sequence-1 (ETS1), E-cadherin, N-cadherin and Vimentin in BC cells. Cell proliferation was measured using the cell counting kit-8 assay and the bromodeoxyuridine assay. Cell migration and invasion were detected by Transwell assay. The interactions between circ_0000291 and miR-326, miR-326 and ETS1 were verified using bioinformatics prediction, the dual-luciferase reporter gene assay or/and RNA binding protein immunoprecipitation assay. We demonstrated that circ_0000291 was significantly upregulated in BC, and its high expression was positively correlated with T stage and local lymph node metastasis. Functional assays validated that circ_0000291 promoted BC cell proliferation, migration and invasion. The mechanism studies indicated that circ_0000291 could decoy miR-326 and in turn upregulate the expression of ETS1. In conclusion, circ_0000291 is the novel oncogenic circRNA and promotes BC progression via modulating the miR-326/ETS1 axis.", "gemini": "Các nghiên cứu tích lũy xác thực rằng RNA tròn (circRNA) có liên quan đến sự tiến triển của ung thư. Tuy nhiên, vai trò của chúng trong ung thư vú (BC) vẫn chưa được biết đến nhiều. Trong nghiên cứu này, phản ứng chuỗi polymerase thời gian thực đã được sử dụng để phát hiện các biểu hiện circ_0000291 và miR-326 trong các mô và tế bào BC. Đã phân tích mối tương quan giữa mức độ biểu hiện của circ_0000291 và các thông số lâm sàng của bệnh nhân BC. Western blot được sử dụng để phát hiện biểu hiện của trình tự đặc hiệu biến đổi E26-1 (ETS1), E-cadherin, N-cadherin và Vimentin trong các tế bào BC. Sự tăng sinh tế bào được đo bằng xét nghiệm bộ đếm tế bào-8 và xét nghiệm bromodeoxyuridine. Di chuyển và xâm lấn tế bào được phát hiện bằng xét nghiệm Transwell. Các tương tác giữa circ_0000291 và miR-326, miR-326 và ETS1 đã được xác minh bằng dự đoán tin sinh học, xét nghiệm gen báo cáo kép luciferase hoặc/và xét nghiệm kết tủa miễn dịch protein liên kết RNA. Chúng tôi đã chứng minh rằng circ_0000291 được điều hòa tăng lên đáng kể trong BC và biểu hiện cao của nó có tương quan thuận với giai đoạn T và di căn hạch bạch huyết tại chỗ. Các xét nghiệm chức năng đã xác nhận rằng circ_0000291 thúc đẩy sự tăng sinh, di chuyển và xâm lấn tế bào BC. Các nghiên cứu cơ chế chỉ ra rằng circ_0000291 có thể giải mã miR-326 và ngược lại điều hòa tăng biểu hiện của ETS1. Tóm lại, circ_0000291 là circRNA gây ung thư mới và thúc đẩy sự tiến triển của BC thông qua việc điều chỉnh trục miR-326/ETS1."} +{"idx": 1870321, "en": "In the first year of life, the ability to engage in sustained synchronous interactions develops as infants learn to match social partner behaviors and sequentially regulate their behaviors in response to others. Difficulties developing competence in these early social building blocks can impact later language skills, joint attention, and emotion regulation. For children at elevated risk for autism spectrum disorder (ASD), early dyadic synchrony and responsiveness difficulties may be indicative of emerging ASD and/or developmental concerns. As part of a prospective developmental monitoring study, infant siblings of children with ASD (high-risk group n = 104) or typical development (low-risk group n = 71), and their mothers completed a standardized play task when infants were 6, 9, and/or 12 months of age. These interactions were coded for the frequency and duration of infant and mother gaze, positive affect, and vocalizations, respectively. Using these codes, theory-driven composites were created to index dyadic synchrony and infant/maternal responsiveness. Multilevel models revealed significant risk group differences in dyadic synchrony and infant responsiveness by 12 months of age. In addition, high-risk infants with higher dyadic synchrony and infant responsiveness at 12 months received significantly higher receptive and expressive language scores at 36 months. The findings of the present study highlight that promoting dyadic synchrony and responsiveness may aid in advancing optimal development in children at elevated risk for autism. LAY SUMMARY: In families raising children with an autism spectrum disorder (ASD), younger siblings are at elevated risks for social communication difficulties. The present study explored whether social-communication differences were evident during a parent-child play task at 6, 9, and 12 months of age. For infant siblings of children with ASD, social differences during play were observed by 12 months of age and may inform ongoing monitoring and intervention efforts.", "gemini": "Trong năm đầu đời, khả năng tham gia vào các tương tác đồng bộ có tính bền vững phát triển khi trẻ sơ sinh học cách khớp hành vi của bạn tình xã hội và điều chỉnh hành vi của chúng theo trình tự để đáp lại người khác. Khó khăn trong việc phát triển năng lực trong những khối xây dựng xã hội ban đầu này có thể ảnh hưởng đến các kỹ năng ngôn ngữ sau này, sự chú ý chung và điều hòa cảm xúc. Đối với trẻ em có nguy cơ mắc chứng rối loạn phổ tự kỷ (ASD) cao, những khó khăn về sự đồng bộ và khả năng đáp ứng sớm có thể là dấu hiệu của ASD mới nổi và/hoặc các mối quan tâm về phát triển. Là một phần của nghiên cứu theo dõi phát triển có triển vọng, anh chị em sơ sinh của trẻ em mắc ASD (nhóm có nguy cơ cao n = 104) hoặc phát triển bình thường (nhóm có nguy cơ thấp n = 71) và mẹ của chúng đã hoàn thành một nhiệm vụ chơi được chuẩn hóa khi trẻ sơ sinh được 6, 9 và/hoặc 12 tháng tuổi. Những tương tác này được mã hóa về tần suất và thời lượng nhìn chằm chằm của trẻ sơ sinh và người mẹ, tình cảm tích cực và giọng nói tương ứng. Sử dụng các mã này, các hợp chất do lý thuyết thúc đẩy đã được tạo ra để lập chỉ mục sự đồng bộ của dyadic và khả năng đáp ứng của trẻ sơ sinh/mẹ. Các mô hình đa cấp cho thấy sự khác biệt đáng kể về nhóm rủi ro trong sự đồng bộ của dyadic và khả năng đáp ứng của trẻ sơ sinh ở độ tuổi 12 tháng. Ngoài ra, trẻ sơ sinh có nguy cơ cao có sự đồng bộ của dyadic và khả năng đáp ứng của trẻ sơ sinh cao hơn ở độ tuổi 12 tháng nhận được điểm số ngôn ngữ tiếp thu và biểu đạt cao hơn đáng kể ở độ tuổi 36 tháng. Những phát hiện của nghiên cứu hiện tại nhấn mạnh rằng việc thúc đẩy sự đồng bộ và khả năng đáp ứng của dyadic có thể hỗ trợ thúc đẩy sự phát triển tối ưu ở trẻ em có nguy cơ mắc chứng tự kỷ cao. TÓM TẮT NGHIÊN CỨU: Trong các gia đình nuôi dạy trẻ mắc chứng rối loạn phổ tự kỷ (ASD), những đứa trẻ em út có nguy cơ cao gặp khó khăn về giao tiếp xã hội. Nghiên cứu hiện tại đã khám phá xem sự khác biệt về giao tiếp xã hội có rõ ràng trong nhiệm vụ chơi giữa cha mẹ và con ở độ tuổi 6, 9 và 12 tháng hay không. Đối với anh chị em sơ sinh của trẻ em mắc ASD, sự khác biệt về mặt xã hội trong khi chơi đã được quan sát thấy ở độ tuổi 12 tháng và có thể cung cấp thông tin cho các nỗ lực theo dõi và can thiệp đang diễn ra."} +{"idx": 1870322, "en": "Abnormal sensory responses are a DSM-5 symptom of autism spectrum disorder (ASD), and research findings demonstrate altered sensory processing in ASD. Beyond difficulties with processing information within single sensory domains, including both hypersensitivity and hyposensitivity, difficulties in multisensory processing are becoming a core issue of focus in ASD. These difficulties may be targeted by treatment approaches such as \"sensory integration,\" which is frequently applied in autism treatment but not yet based on clear evidence. Recently, psychophysical data have emerged to demonstrate multisensory deficits in some children with ASD. Unlike deficits in social communication, which are best understood in humans, sensory and multisensory changes offer a tractable marker of circuit dysfunction that is more easily translated into animal model systems to probe the underlying neurobiological mechanisms. Paralleling experimental paradigms that were previously applied in humans and larger mammals, we and others have demonstrated that multisensory function can also be examined behaviorally in rodents. Here, we review the sensory and multisensory difficulties commonly found in ASD, examining laboratory findings that relate these findings across species. Next, we discuss the known neurobiology of multisensory integration, drawing largely on experimental work in larger mammals, and extensions of these paradigms into rodents. Finally, we describe emerging investigations into multisensory processing in genetic mouse models related to autism risk. By detailing findings from humans to mice, we highlight the advantage of multisensory paradigms that can be easily translated across species, as well as the potential for rodent experimental systems to reveal opportunities for novel treatments. LAY SUMMARY: Sensory and multisensory deficits are commonly found in ASD and may result in cascading effects that impact social communication. By using similar experiments to those in humans, we discuss how studies in animal models may allow an understanding of the brain mechanisms that underlie difficulties in multisensory integration, with the ultimate goal of developing new treatments. Autism Res 2020, 13: 1430-1449. © 2020 International Society for Autism Research, Wiley Periodicals, Inc.", "gemini": "Phản ứng cảm giác bất thường là một triệu chứng của DSM-5 về chứng rối loạn phổ tự kỷ (ASD) và các phát hiện nghiên cứu chứng minh quá trình xử lý cảm giác bị thay đổi trong ASD. Ngoài những khó khăn trong việc xử lý thông tin trong các miền cảm giác đơn lẻ, bao gồm cả quá mẫn cảm và giảm mẫn cảm, những khó khăn trong quá trình xử lý đa giác quan đang trở thành vấn đề trọng tâm trong ASD. Những khó khăn này có thể được nhắm mục tiêu bằng các phương pháp điều trị như \"tích hợp cảm giác\", thường được áp dụng trong điều trị chứng tự kỷ nhưng vẫn chưa dựa trên bằng chứng rõ ràng. Gần đây, dữ liệu tâm lý học đã xuất hiện để chứng minh sự thiếu hụt đa giác quan ở một số trẻ mắc ASD. Không giống như những khiếm khuyết trong giao tiếp xã hội, được hiểu rõ nhất ở con người, những thay đổi về cảm giác và đa giác quan cung cấp một dấu hiệu dễ hiểu về rối loạn chức năng mạch máu dễ dàng chuyển thành các hệ thống mô hình động vật hơn để thăm dò các cơ chế thần kinh sinh học cơ bản. Song song với các mô hình thực nghiệm trước đây được áp dụng ở người và động vật có vú lớn hơn, chúng tôi và những người khác đã chứng minh rằng chức năng đa giác quan cũng có thể được kiểm tra về mặt hành vi ở loài gặm nhấm. Tại đây, chúng tôi xem xét những khó khăn về cảm giác và đa giác quan thường gặp trong ASD, kiểm tra các phát hiện trong phòng thí nghiệm liên quan đến những phát hiện này giữa các loài. Tiếp theo, chúng tôi thảo luận về thần kinh học đã biết về sự tích hợp đa giác quan, chủ yếu dựa trên công trình thực nghiệm ở động vật có vú lớn hơn và mở rộng các mô hình này thành loài gặm nhấm. Cuối cùng, chúng tôi mô tả các cuộc điều tra mới nổi về quá trình xử lý đa giác quan trong các mô hình chuột di truyền liên quan đến nguy cơ mắc chứng tự kỷ. Bằng cách trình bày chi tiết các phát hiện từ con người đến chuột, chúng tôi nhấn mạnh lợi thế của các mô hình đa giác quan có thể dễ dàng chuyển đổi giữa các loài, cũng như tiềm năng của các hệ thống thực nghiệm ở loài gặm nhấm để khám phá các cơ hội cho các phương pháp điều trị mới. TÓM TẮT NGHIÊN CỨU: Những khiếm khuyết về cảm giác và đa giác quan thường gặp trong ASD và có thể dẫn đến những tác động theo tầng ảnh hưởng đến giao tiếp xã hội. Bằng cách sử dụng các thí nghiệm tương tự như ở người, chúng tôi thảo luận về cách các nghiên cứu trên mô hình động vật có thể cho phép hiểu được các cơ chế não nằm bên dưới những khó khăn trong quá trình tích hợp đa giác quan, với mục tiêu cuối cùng là phát triển các phương pháp điều trị mới. Nghiên cứu về chứng tự kỷ năm 2020, 13: 1430-1449. © 2020 Hiệp hội nghiên cứu chứng tự kỷ quốc tế, Tạp chí Wiley, Inc."} +{"idx": 1870323, "en": "The purpose was to determine whether postactivation performance enhancement is specific to the muscle being conditioned or if it is also observed within the homologous muscles of the contralateral limb (after accounting for the warm-up and random error). We also investigated whether this differed based on training status or muscle size. One hundred seven participants (75 untrained; 32 trained) participated in four sessions. Visit 1 included baseline measurements and familiarization. Visits 2-4 included the completion of one of the three experimental conditions: (a) control, (b) same side and (c) crossover completed in a randomized order. The control condition completed all testing except for the conditioning contraction. The same side condition completed the conditioning contraction on the same side as the strength test. The crossover condition completed the conditioning on the arm opposite to the strength test. The variable of interest was the change from baseline in isokinetic strength. Our analysis indicated that of the hypotheses compared, the posterior probabilities (posterior probability of 0.506) favoured the hypothesis that the effect was local and greatest in those who were resistance trained [mean (SD) of 1.4 (2.2) Nm over the control in those resistance trained]. We found no relationship between muscle size and postactivation performance enhancement. In conclusion, there is an influence of training status pertaining to the postactivation performance enhancement effect but no influence from baseline muscle size. It appears unlikely that the effect is due to a systemic mechanism.", "gemini": "Mục đích là để xác định xem sự tăng cường hiệu suất sau kích hoạt có đặc hiệu đối với cơ được điều hòa hay không hoặc liệu nó cũng được quan sát thấy trong các cơ tương đồng của chi đối diện (sau khi tính đến quá trình khởi động và lỗi ngẫu nhiên). Chúng tôi cũng điều tra xem điều này có khác nhau dựa trên trạng thái luyện tập hoặc kích thước cơ hay không. Một trăm bảy người tham gia (75 người chưa được đào tạo; 32 người đã được đào tạo) đã tham gia bốn buổi. Buổi 1 bao gồm các phép đo cơ bản và làm quen. Các buổi 2-4 bao gồm việc hoàn thành một trong ba điều kiện thực nghiệm: (a) kiểm soát, (b) cùng một phía và (c) chéo được hoàn thành theo thứ tự ngẫu nhiên. Điều kiện kiểm soát đã hoàn thành tất cả các thử nghiệm ngoại trừ co bóp điều hòa. Điều kiện cùng một phía hoàn thành co bóp điều hòa cùng phía với bài kiểm tra sức mạnh. Điều kiện giao nhau hoàn thành điều hòa ở cánh tay đối diện với bài kiểm tra sức mạnh. Biến quan được quan tâm là sự thay đổi từ giá trị cơ bản về sức mạnh đẳng trương. Phân tích của chúng tôi chỉ ra rằng trong số các giả thuyết được so sánh, xác suất sau (xác suất sau là 0,506) ủng hộ giả thuyết cho rằng hiệu ứng là cục bộ và lớn nhất ở những người được rèn luyện sức đề kháng [trung bình (SD) là 1,4 (2,2) Nm so với nhóm kiểm soát ở những người được rèn luyện sức đề kháng]. Chúng tôi không tìm thấy mối quan hệ nào giữa kích thước cơ và sự tăng cường hiệu suất sau kích hoạt. Tóm lại, có một ảnh hưởng của trạng thái luyện tập liên quan đến hiệu ứng tăng cường hiệu suất sau kích hoạt nhưng không có ảnh hưởng từ kích thước cơ ban đầu. Có vẻ như không có khả năng là hiệu ứng là do một cơ chế toàn thân."} +{"idx": 1870324, "en": "BACKGROUND The current outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, named coronavirus disease 19 (COVID-19), is not the first well-known spillover of an animal originated virus to infect humans. However, one of the few to make such a fast jump in a powerful evolutionary shortcut. The incredible pattern of aggressiveness worldwide since the beginning of the outbreak is that up to 20% of those infected need hospitalization and 5% evolve to critical conditions, not limited to respiratory-related issues, but rather to systemic involvement. OBJECTIVE This study aims to summarize the current knowledge about the effects of SARS-CoV-2 infection on the male genitourinary tract. MATERIALS AND METHODS A narrative review was carried out to identify articles on the SARS-CoV-2 infection on the male genitourinary system. RESULTS Considerations were made about the molecular characteristics of SARS-CoV-2 and immune response to coronavirus. We discussed the influence of the virus on the urinary system, potential mechanisms of COVID-19- related acute kidney injury (AKI), and the role of cytokine release syndrome on the renal pathophysiology of the disease. In the male reproductive tract, it was discussed the testis' vulnerability to SARS-CoV-2 invasion and the possible adverse effects on its function and the seminal findings of COVID-19. DISCUSSION AND CONCLUSION During the COVID-19 pandemic, an international coordinated scientific effort must arise to understand the role of the urogenital system in the SARS-CoV-2 infection in the clinical setting.", "gemini": "BỐI CẢNH Sự bùng phát hiện tại của hội chứng hô hấp cấp tính nặng do virus corona 2 (SARS-CoV-2), được đặt tên là bệnh do virus corona 2019 (COVID-19), không phải là sự tràn lan đầu tiên được biết đến của một loại virus có nguồn gốc từ động vật gây nhiễm trùng ở người. Tuy nhiên, đây là một trong số ít loại virus có thể thực hiện một bước nhảy vọt nhanh như vậy trong một lối tắt tiến hóa mạnh mẽ. Mô hình hung hăng đáng kinh ngạc trên toàn thế giới kể từ khi bắt đầu bùng phát là có tới 20% số người bị nhiễm bệnh cần nhập viện và 5% tiến triển thành tình trạng nguy kịch, không chỉ giới hạn ở các vấn đề liên quan đến hô hấp mà còn liên quan đến toàn thân. MỤC TIÊU Nghiên cứu này nhằm mục đích tóm tắt các kiến thức hiện tại về tác động của nhiễm SARS-CoV-2 đối với đường sinh dục tiết niệu ở nam giới. VẬT LIỆU VÀ PHƯƠNG PHÁP Một đánh giá tường thuật đã được thực hiện để xác định các bài báo về nhiễm SARS-CoV-2 trên hệ thống sinh dục tiết niệu của nam giới. KẾT QUẢ Các cân nhắc đã được đưa ra về các đặc điểm phân tử của SARS-CoV-2 và phản ứng miễn dịch đối với virus corona. Chúng tôi đã thảo luận về ảnh hưởng của vi-rút đối với hệ tiết niệu, các cơ chế tiềm ẩn của tổn thương thận cấp (AKI) liên quan đến COVID-19 và vai trò của hội chứng giải phóng cytokine đối với bệnh lý sinh ở thận của bệnh. Trong đường sinh sản của nam giới, đã thảo luận về tính dễ bị tổn thương của tinh hoàn đối với sự xâm nhập của SARS-CoV-2 và những tác động có hại có thể xảy ra đối với chức năng của nó và các phát hiện về tinh dịch của COVID-19. THẢO LUẬN VÀ KẾT LUẬN Trong đại dịch COVID-19, một nỗ lực khoa học phối hợp quốc tế phải được thực hiện để hiểu vai trò của hệ thống sinh dục tiết niệu trong nhiễm SARS-CoV-2 trong bối cảnh lâm sàng."} +{"idx": 1870325, "en": "As an increasing number of COVID-19 cases, there were changes in the number of patients who attended the dermatology outpatient clinics. We aimed to investigate the change profiles of dermatologic diseases in the first and second months of the COVID-19 pandemic in Turkey by comparing with the corresponding period of the previous year. The total number and diagnosis of patients, who attended a tertiary care hospital for the dermatology outpatient clinic between 1 April 2020 and 31 May 2020, were included in this study. These data were compared with the corresponding period of the previous year. The percentage of the patients with scabies, contact dermatitis, psoriasis, pityriasis rosea, urticaria, and alopecia areata were statistically significantly increased a month after the occurrence of the COVID-19 pandemic, while the percentage of patients with scabies, alopecia areata, telogen effluvium, acne vulgaris, and xerosis cutis were statistically significantly increased 2 months after the occurrence of the COVID-19 pandemic (P < 0.05). An increase in the number of certain diseases such as urticaria and pityriasis rosea may indicate the risk of asymptomatic COVID-19 carriage in these patients. Polymerase chain reaction (PCR) and/or antibody-based further studies should be performed to explore whether certain dermatologic diseases are related to asymptomatic COVID-19 cases.", "gemini": "Khi số ca mắc COVID-19 ngày càng tăng, số bệnh nhân đến khám tại các phòng khám da liễu ngoại trú có sự thay đổi. Chúng tôi nhằm mục đích điều tra hồ sơ thay đổi của các bệnh da liễu trong tháng đầu tiên và tháng thứ hai của đại dịch COVID-19 tại Thổ Nhĩ Kỳ bằng cách so sánh với cùng kỳ năm trước. Tổng số và chẩn đoán của bệnh nhân đến khám tại bệnh viện chăm sóc sức khỏe ba cấp cho phòng khám da liễu ngoại trú trong khoảng thời gian từ ngày 1 tháng 4 năm 2020 đến ngày 31 tháng 5 năm 2020 đã được đưa vào nghiên cứu này. Những dữ liệu này đã được so sánh với cùng kỳ năm trước. Tỷ lệ bệnh nhân mắc ghẻ, viêm da tiếp xúc, bệnh vẩy nến, hồng ban vảy phấn, mày đay và rụng tóc từng vùng tăng đáng kể về mặt thống kê một tháng sau khi xảy ra đại dịch COVID-19, trong khi tỷ lệ bệnh nhân mắc ghẻ, rụng tóc từng vùng, rụng tóc telogen, mụn trứng cá thông thường và khô da tăng đáng kể về mặt thống kê 2 tháng sau khi xảy ra đại dịch COVID-19 (P < 0,05). Sự gia tăng số lượng một số bệnh nhất định như mày đay và hồng ban vảy phấn có thể chỉ ra nguy cơ mang COVID-19 không triệu chứng ở những bệnh nhân này. Nên tiến hành phản ứng chuỗi polymerase (PCR) và/hoặc các nghiên cứu sâu hơn dựa trên kháng thể để tìm hiểu xem một số bệnh da liễu nhất định có liên quan đến các trường hợp COVID-19 không triệu chứng hay không."} +{"idx": 1870326, "en": "The pathophysiology of polycystic ovary syndrome (PCOS) is characterized by granulosa cell (GC) dysfunction. m6 A modification affects GC function in patients with premature ovarian insufficiency (POI), but the role of m6 A modification in PCOS is unknown. The purpose of the prospective comparative study was to analyse the m6 A profile of the luteinized GCs from normovulatory women and non-obese PCOS patients following controlled ovarian hyperstimulation. RNA m6 A methylation levels were measured by m6 A quantification assay in the luteinized GCs of the controls and PCOS patients. Then, m6 A profiles were analysed by methylated RNA immunoprecipitation sequencing (MeRIP-seq). We reported that the m6 A level was increased in the luteinized GCs of PCOS patients. Comparative analysis revealed differences between the m6 A profiles from the luteinized GC of the controls and PCOS patients. We identified FOXO3 mRNA with reduced m6 A modification in the luteinized GCs of PCOS patients. Selectively knocking down m6 A methyltransferases or demethylases altered expression of FOXO3 in the luteinized GCs from the controls, but did not in PCOS patients. These suggested an absence of m6 A-mediated transcription of FOXO3 in the luteinized GCs of PCOS patients. Furthermore, we demonstrated that the involvement of m6 A in the stability of the FOXO3 mRNA that is regulated via a putative methylation site in the 3'-UTR only in the luteinized GCs of the controls. In summary, our findings showed that altered m6 A modification was involved in up-regulated expression of FOXO3 mRNA in the luteinized GCs from non-obese PCOS patients following controlled ovarian hyperstimulation.", "gemini": "Bệnh sinh của hội chứng buồng trứng đa nang (PCOS) được đặc trưng bởi rối loạn chức năng tế bào hạt (GC). Sửa đổi m6 A ảnh hưởng đến chức năng GC ở những bệnh nhân suy buồng trứng sớm (POI), nhưng vai trò của sửa đổi m6 A trong PCOS vẫn chưa được biết. Mục đích của nghiên cứu so sánh có triển vọng là phân tích hồ sơ m6 A của các GC bị hoàng thể hóa từ những phụ nữ có chu kỳ kinh nguyệt bình thường và những bệnh nhân PCOS không béo phì sau khi kích thích buồng trứng có kiểm soát. Nồng độ metyl hóa m6 A của RNA được đo bằng xét nghiệm định lượng m6 A trong các GC bị hoàng thể hóa của nhóm đối chứng và bệnh nhân PCOS. Sau đó, hồ sơ m6 A được phân tích bằng giải trình tự kết tủa miễn dịch RNA đã metyl hóa (MeRIP-seq). Chúng tôi báo cáo rằng mức m6 A tăng trong các GC bị hoàng thể hóa của bệnh nhân PCOS. Phân tích so sánh cho thấy sự khác biệt giữa hồ sơ m6 A từ GC bị hoàng thể hóa của nhóm đối chứng và bệnh nhân PCOS. Chúng tôi đã xác định mRNA FOXO3 có sửa đổi m6 A giảm trong các GC bị hoàng thể hóa của bệnh nhân PCOS. Việc loại bỏ một cách có chọn lọc các metyltransferase m6 A hoặc demethylase đã làm thay đổi biểu hiện của FOXO3 trong các GC bị hoàng thể hóa từ nhóm đối chứng, nhưng không ở bệnh nhân PCOS. Những điều này cho thấy không có phiên mã FOXO3 do m6 A trung gian trong các GC bị hoàng thể hóa của bệnh nhân PCOS. Hơn nữa, chúng tôi đã chứng minh rằng sự tham gia của m6 A vào tính ổn định của mRNA FOXO3 được điều chỉnh thông qua một vị trí metyl hóa có thể có trong 3'-UTR chỉ trong các GC bị hoàng thể hóa của nhóm đối chứng. Tóm lại, những phát hiện của chúng tôi cho thấy rằng sửa đổi m6 A bị thay đổi liên quan đến biểu hiện tăng lên của mRNA FOXO3 trong các GC bị hoàng thể hóa từ bệnh nhân PCOS không béo phì sau khi kích thích buồng trứng có kiểm soát."} +{"idx": 1870327, "en": "Cancer immunotherapy often reflects the improvement in both short-term risk reduction and long-term survival. In this scenario, a mixture cure model can be used for the trial design. However, the hazard functions based on the mixture cure model between two groups will ultimately crossover. Thus, the conventional assumption of proportional hazards may be violated and study design using standard log-rank test (LRT) could lose power if the main interest is to detect the improvement of long-term survival. In this paper, we propose a change sign weighted LRT for the trial design. We derived a sample size formula for the weighted LRT, which can be used for designing cancer immunotherapy trials to detect both short-term risk reduction and long-term survival. Simulation studies are conducted to compare the efficiency between the standard LRT and the change sign weighted LRT.", "gemini": "Miễn dịch ung thư thường phản ánh sự cải thiện cả về giảm nguy cơ ngắn hạn và kéo dài sự sống. Trong trường hợp này, có thể sử dụng mô hình chữa bệnh hỗn hợp cho thiết kế thử nghiệm. Tuy nhiên, các hàm nguy cơ dựa trên mô hình chữa bệnh hỗn hợp giữa hai nhóm cuối cùng sẽ giao nhau. Do đó, giả định thông thường về các mối nguy hiểm theo tỷ lệ có thể bị vi phạm và thiết kế nghiên cứu sử dụng kiểm định log-rank chuẩn (LRT) có thể mất hiệu lực nếu mục đích chính là phát hiện sự cải thiện khả năng sống sót lâu dài. Trong bài báo này, chúng tôi đề xuất một LRT có trọng số thay đổi dấu hiệu cho thiết kế thử nghiệm. Chúng tôi đã tìm ra công thức kích thước mẫu cho LRT có trọng số, có thể được sử dụng để thiết kế các thử nghiệm miễn dịch ung thư nhằm phát hiện cả tình trạng giảm nguy cơ trong thời gian ngắn và kéo dài sự sống. Các nghiên cứu mô phỏng được tiến hành để so sánh hiệu quả giữa LRT chuẩn và LRT có trọng số thay đổi dấu hiệu."} +{"idx": 1870328, "en": "Skin cutaneous melanoma (SKCM) is a multifactorial disease that presents a poor prognosis due to its rapid progression towards metastasis. This study focused on the identification of prognostic differentially expressed genes (DEGs) between primary and metastatic SKCM. DEGs were obtained using three chip data sets from the Gene Expression Omnibus database. The protein-protein interaction network was described by STRING and Cytoscape. Kaplan-Meier curves were implemented to evaluate survival benefits within distinct groups. A total of 258 DEGs were distinguished as possible candidate biomarkers. Besides, survival curves indicated that DSG3, DSC3, PKP1, EVPL, IVL, FLG, SPRR1A and SPRR1B were of significant value to predict the metastatic transformation of melanoma. To further validate our hypotheses, functional enrichment and significant pathways of the hub genes were performed to indicate that the most involved considerable path. In summary, this study identified substantial DEGs participating in melanoma metastasis. DGS3, DSC3, PKP1, EVPL, IVL, FLG, SPRR1A and SPRR1B may be considered as new biomarkers in the therapeutics of metastatic melanoma, which might help us predict the potential metastatic capability of SKCM patients, thus provide earlier precautionary treatments. However, further experiments are still required to support the specific mechanisms of these hub genes.", "gemini": "U hắc tố da (SKCM) là một căn bệnh đa yếu tố có tiên lượng xấu do tiến triển nhanh thành di căn. Nghiên cứu này tập trung vào việc xác định các gen biểu hiện khác biệt có giá trị tiên lượng (DEG) giữa SKCM nguyên phát và di căn. DEG được thu thập bằng cách sử dụng ba bộ dữ liệu chip từ cơ sở dữ liệu Gene Expression Omnibus. Mạng lưới tương tác protein-protein được mô tả bởi STRING và Cytoscape. Các đường cong Kaplan-Meier được thực hiện để đánh giá lợi ích sống còn trong các nhóm riêng biệt. Tổng cộng 258 DEG được phân biệt là các ứng cử viên sinh học có thể. Bên cạnh đó, các đường cong sống còn cho thấy DSG3, DSC3, PKP1, EVPL, IVL, FLG, SPRR1A và SPRR1B có giá trị đáng kể để dự đoán quá trình biến đổi di căn của bệnh u hắc tố. Để xác thực thêm các giả thuyết của mình, chúng tôi đã thực hiện làm giàu chức năng và các con đường quan trọng của các gen trung tâm để chỉ ra con đường đáng kể liên quan nhất. Tóm lại, nghiên cứu này đã xác định được các DEG quan trọng tham gia vào quá trình di căn của bệnh u hắc tố. DGS3, DSC3, PKP1, EVPL, IVL, FLG, SPRR1A và SPRR1B có thể được coi là các sinh học đánh dấu mới trong điều trị bệnh u hắc tố di căn, có thể giúp chúng ta dự đoán khả năng di căn tiềm ẩn của bệnh nhân SKCM, do đó cung cấp các phương pháp điều trị phòng ngừa sớm hơn. Tuy nhiên, vẫn cần các thí nghiệm sâu hơn để hỗ trợ các cơ chế cụ thể của các gen trung tâm này."} +{"idx": 1870329, "en": "Hypertension and chronic kidney disease (CKD) are serious interrelated public health problems. Despite the monitoring and control of high blood pressure, symptoms of CKD are not usually apparent in its early stages. Previously, we reported the utility of urinary vanin-1 as an early biomarker of kidney injury in spontaneously hypertensive rats, but it remains unknown whether urinary vanin-1 is associated with CKD in humans. In this study, we estimated associations between urinary vanin-1 and parameters of kidney function in a cross-sectional study of hypertensive patients. We measured concentrations of vanin-1 using spot urine from 147 adult hypertensive patients (mean age, 72.8 years; 39.5% women). Patients were divided into 2 groups based on the median of the estimated glomerular filtration rate (eGFR). The group with eGFR < 60 mL/min per 1.73 m2 showed significantly higher levels of urinary vanin-1 than those with eGFR ≥ 60 mL/min per 1.73 m2. On univariate analysis, urinary vanin-1 as well as neutrophil gelatinase-associated lipocalin (NGAL) showed significant negative correlations with eGFR; however, multivariate analysis revealed that urinary vanin-1, but not NGAL, significantly correlated with eGFR. In addition, urinary vanin-1 had a significant positive correlation with the urinary protein-to-creatinine ratio (UPCR) (r = 0.21; P = .021) and albumin-to-creatinine ratio (UACR) (r = 0.61; P < .01). In conclusion, urinary vanin-1 is associated with lower eGFR and higher UPCR and UACR, and might be a potential marker of decreased kidney function in hypertensive patients. Further studies are needed to confirm these findings.", "gemini": "Tăng huyết áp và bệnh thận mãn tính (CKD) là những vấn đề sức khỏe cộng đồng nghiêm trọng có mối quan hệ chặt chẽ với nhau. Mặc dù theo dõi và kiểm soát huyết áp cao, nhưng các triệu chứng của CKD thường không rõ ràng trong giai đoạn đầu. Trước đây, chúng tôi đã báo cáo về tính hữu ích của vanin-1 trong nước tiểu như một sinh học đánh dấu sớm của tổn thương thận ở chuột tăng huyết áp tự phát, nhưng vẫn chưa biết vanin-1 trong nước tiểu có liên quan đến CKD ở người hay không. Trong nghiên cứu này, chúng tôi ước tính mối liên quan giữa vanin-1 trong nước tiểu và các thông số chức năng thận trong một nghiên cứu cắt ngang ở những bệnh nhân tăng huyết áp. Chúng tôi đã đo nồng độ vanin-1 bằng cách sử dụng nước tiểu tại chỗ từ 147 bệnh nhân tăng huyết áp trưởng thành (tuổi trung bình 72,8; 39,5% là phụ nữ). Bệnh nhân được chia thành 2 nhóm dựa trên giá trị trung bình của tốc độ lọc cầu thận ước tính (eGFR). Nhóm có eGFR < 60 mL/phút trên 1,73 m2 cho thấy nồng độ vanin-1 trong nước tiểu cao hơn đáng kể so với nhóm có eGFR ≥ 60 mL/phút trên 1,73 m2. Trên phân tích đơn biến, cả vanin-1 trong nước tiểu cũng như lipocalin liên quan đến gelatinase của bạch cầu trung tính (NGAL) đều cho thấy mối tương quan âm đáng kể với eGFR; tuy nhiên, phân tích đa biến cho thấy vanin-1 trong nước tiểu, nhưng không phải NGAL, có mối tương quan đáng kể với eGFR. Ngoài ra, vanin-1 trong nước tiểu có mối tương quan dương đáng kể với tỷ lệ protein-creatinin trong nước tiểu (UPCR) (r = 0,21; P = 0,021) và tỷ lệ albumin-creatinin (UACR) (r = 0,61; P < 0,01). Tóm lại, vanin-1 trong nước tiểu có liên quan đến eGFR thấp hơn và UPCR và UACR cao hơn, và có thể là một dấu hiệu tiềm ẩn của chức năng thận suy giảm ở bệnh nhân tăng huyết áp. Cần có thêm các nghiên cứu để xác nhận những phát hiện này."} +{"idx": 1870330, "en": "A new DNA architecture addresses the question, how far energy migrates in DNA and forms cyclobutane pyrimidine dimers (CPDs) as photodamages causing skin cancer. The 3-methoxyxanthone nucleoside allows site-selective photoenergy injection into DNA. The designated CPD site lacks the phosphodiester bond and can be placed in defined distances. The CPD formation links two oligonucleotides together and allows probing by gel electrophoresis. We obtained a sigmoidal distance dependence with R0 of 25±3 Å. Below R0 , short-range energy migration occurs with high CPD yields and shallow distance dependence, characteristic for a coherent process. 5-methyl-C as epigenetic modification on the 3'-side facilitates CPD formation. Above R0 , long-range incoherent energy migration occurs over 30 A-T pairs (105.4 Å). The evidence of long-range CPD formation is fundamental for our understanding of DNA photodamaging. Open access funding enabled and organized by Projekt DEAL.", "gemini": "Một cấu trúc DNA mới giải quyết câu hỏi, năng lượng di chuyển trong DNA xa đến mức nào và tạo thành các dimer pyrimidine cyclobutane (CPD) như những tổn thương do bức xạ gây ung thư da. Nucleoside 3-methoxyxanthone cho phép tiêm năng lượng ánh sáng chọn lọc vào DNA. Vị trí CPD được chỉ định không có liên kết phosphodieste và có thể được đặt ở khoảng cách xác định. Sự hình thành CPD liên kết hai oligonucleotide với nhau và cho phép thăm dò bằng điện di gel. Chúng tôi thu được sự phụ thuộc khoảng cách hình sin với R0 là 25 ± 3 Å. Dưới R0, sự di chuyển năng lượng tầm ngắn xảy ra với năng suất CPD cao và sự phụ thuộc khoảng cách nông, đặc trưng cho một quá trình mạch lạc. 5-methyl-C như sửa đổi biểu sinh ở phía 3' tạo thuận lợi cho sự hình thành CPD. Trên R0, sự di chuyển năng lượng không mạch lạc tầm xa xảy ra trên 30 cặp A-T (105,4 Å). Bằng chứng về sự hình thành CPD tầm xa có vai trò cơ bản để chúng ta hiểu về tổn thương DNA do bức xạ. Khoản tài trợ tiếp cận mở được kích hoạt và tổ chức bởi Projekt DEAL."} +{"idx": 1870331, "en": "BACKGROUND Endometrial cancer is one of the three most common malignancies in the female genital tract. Previous studies have demonstrated the association between heparanase (HPSE, OMIM 604,724) single-nucleotide polymorphism (SNP) and cancer risk in several cancers. However, its role in endometrial cancer remains unclear. The present study investigated the effects of HPSE SNPs on the susceptibility and clinicopathological parameters in patients with endometrial cancer. METHODS HPSE SNPs of rs4693608 (G > A) and rs4364254 (C > T) were analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in 270 endometrial cancer patients and 320 healthy controls. RESULTS The investigation indicated that the HPSE SNP rs4693608 with GG showed a protective effect from EC in both codominant (adjusted OR = 0.41, 95%CI = 0.21-0.81, p = .026) and recessive models (adjusted OR = 0.43, 95%CI = 0.22-0.82, p = .0076). No significant differences were found in the incidences of EC patients with the rs4364254 polymorphisms compared to controls. Moreover, a significantly increased distribution of A/A (rs4693608) was observed in patients with grade ≥ 2 (p = .03) and in patients with positive cervical invasion (p = .042) while patients with T/C (rs4364254) had lower tumor grade. CONCLUSION Our study suggested that HPSE SNP of rs4693608 correlated strongly with susceptibility to EC, and HPSE SNPs might be a potential biomarker for prognosis of endometrial cancer.", "gemini": "BỐI CẢNH Ung thư nội mạc tử cung là một trong ba loại ung thư phổ biến nhất ở đường sinh dục nữ. Các nghiên cứu trước đây đã chứng minh mối liên quan giữa đa hình một nucleotide (SNP) của heparanase (HPSE, OMIM 604.724) và nguy cơ ung thư ở một số loại ung thư. Tuy nhiên, vai trò của nó trong ung thư nội mạc tử cung vẫn chưa rõ ràng. Nghiên cứu hiện tại đã điều tra ảnh hưởng của SNP HPSE đến các thông số về tính nhạy cảm và lâm sàng bệnh lý ở những bệnh nhân mắc ung thư nội mạc tử cung. PHƯƠNG PHÁP Các SNP HPSE của rs4693608 (G> A) và rs4364254 (C> T) đã được phân tích bằng xét nghiệm đa hình độ dài đoạn hạn chế phản ứng chuỗi polymerase (PCR-RFLP) ở 270 bệnh nhân ung thư nội mạc tử cung và 320 đối chứng khỏe mạnh. KẾT QUẢ Cuộc điều tra chỉ ra rằng SNP HPSE rs4693608 với GG cho thấy tác dụng bảo vệ khỏi EC ở cả mô hình trội không hoàn toàn (OR được điều chỉnh = 0,41, 95% CI = 0,21-0,81, p = 0,026) và mô hình lặn (OR được điều chỉnh = 0,43, 95% CI = 0,22-0,82, p = 0,0076). Không có sự khác biệt đáng kể nào về tỷ lệ mắc bệnh EC ở những bệnh nhân có đa hình rs4364254 so với nhóm đối chứng. Hơn nữa, sự phân bố tăng đáng kể của A / A (rs4693608) được quan sát thấy ở những bệnh nhân có độ ≥ 2 (p = 0,03) và ở những bệnh nhân có xâm lấn cổ tử cung dương tính (p = 0,042) trong khi những bệnh nhân có T / C (rs4364254) có độ u thấp hơn. KẾT LUẬN Nghiên cứu của chúng tôi cho thấy SNP HPSE của rs4693608 có mối tương quan mạnh với tính nhạy cảm với EC và SNP HPSE có thể là một dấu ấn sinh học tiềm năng để tiên lượng ung thư nội mạc tử cung."} +{"idx": 1870332, "en": "OBJECTIVES Rapid antiretroviral therapy (ART) initiation is recommended in early HIV infection (EHI), even in the absence of baseline viral resistance test result. We analysed time to viral suppression according to ART regimen started in a cohort of patients diagnosed with EHI. METHODS Clinical records of individuals consecutively diagnosed with EHI between July 2016-June 2018 were reviewed. The distribution of clinical, virological and immunological factors was compared in treatment groups using the Mann-Whitney U-test. RESULTS 262 individuals (97% MSM) were diagnosed with EHI. 58% of patients agreed to start ART within 14 days of diagnosis. Tenofovir-based combinations were prescribed to all patients. DRV/b was the most commonly prescribed third agent (78%), when genotypic resistance testing was not available at time of ART choice. Switching to INSTI was encouraged once VRT became available and 27% switched from DRV/b to INSTI (mainly RAL) within 28 days from ART start. Those receiving INSTI were more likely to have a baseline viral load exceeding 1 million HIV-1 RNA copies/mL compared with those starting with DRV/b. Rapid start with INSTI regimens resulted in quicker viral suppression than when DRV/b was chosen in EHI, even when that was subsequently switched to INSTI. Retention in care following rapid ART start was achieved by all patients at 24 weeks. CONCLUSIONS Starting an INSTI-based ART combination was associated with quicker viral suppression than when a protease inhibitor-based combination was chosen. No differences in the achievement of viral suppression or in retention in care were observed.", "gemini": "MỤC TIÊU Nên bắt đầu liệu pháp kháng retrovirus (ART) nhanh chóng trong giai đoạn đầu nhiễm HIV (EHI), ngay cả khi không có kết quả xét nghiệm kháng thuốc virus ban đầu. Chúng tôi đã phân tích thời gian ức chế virus theo phác đồ ART bắt đầu ở một nhóm bệnh nhân được chẩn đoán mắc EHI. PHƯƠNG PHÁP Hồ sơ lâm sàng của những cá nhân được chẩn đoán mắc EHI liên tiếp trong giai đoạn từ tháng 7 năm 2016 đến tháng 6 năm 2018 đã được xem xét. Sự phân bố của các yếu tố lâm sàng, vi rút và miễn dịch đã được so sánh trong các nhóm điều trị bằng cách sử dụng phép thử U-Mann-Whitney. KẾT QUẢ 262 cá nhân (97% MSM) được chẩn đoán mắc EHI. 58% bệnh nhân đồng ý bắt đầu ART trong vòng 14 ngày kể từ khi chẩn đoán. Các phối hợp dựa trên Tenofovir được kê đơn cho tất cả bệnh nhân. DRV / b là thuốc thứ ba được kê đơn phổ biến nhất (78%), khi xét nghiệm kháng gen không có sẵn tại thời điểm lựa chọn ART. Việc chuyển sang INSTI được khuyến khích sau khi VRT có sẵn và 27% chuyển từ DRV / b sang INSTI (chủ yếu là RAL) trong vòng 28 ngày kể từ khi bắt đầu ART. Những người dùng INSTI có nhiều khả năng có tải lượng virus cơ bản vượt quá 1 triệu bản sao HIV-1 RNA / mL so với những người bắt đầu bằng DRV / b. Bắt đầu nhanh với phác đồ INSTI dẫn đến ức chế virus nhanh hơn so với khi chọn DRV / b ở EHI, ngay cả khi sau đó chuyển sang INSTI. Tất cả bệnh nhân đều được duy trì chăm sóc sau khi bắt đầu ART nhanh chóng ở tuần thứ 24. KẾT LUẬN Bắt đầu kết hợp ART dựa trên INSTI có liên quan đến việc ức chế virus nhanh hơn so với khi chọn kết hợp dựa trên thuốc ức chế protease. Không có sự khác biệt nào về việc đạt được ức chế virus hoặc duy trì chăm sóc được quan sát thấy."} +{"idx": 1870333, "en": "People with autism spectrum disorder (ASD) show atypical attention to social stimuli and aberrant gaze when viewing images of the physical world. However, it is unknown how they perceive the world from a first-person perspective. In this study, we used machine learning to classify photos taken in three different categories (people, indoors, and outdoors) as either having been taken by individuals with ASD or by peers without ASD. Our classifier effectively discriminated photos from all three categories, but was particularly successful at classifying photos of people with >80% accuracy. Importantly, visualization of our model revealed critical features that led to successful discrimination and showed that our model adopted a strategy similar to that of ASD experts. Furthermore, for the first time we showed that photos taken by individuals with ASD contained less salient objects, especially in the central visual field. Notably, our model outperformed classification of these photos by ASD experts. Together, we demonstrate an effective and novel method that is capable of discerning photos taken by individuals with ASD and revealing aberrant visual attention in ASD from a unique first-person perspective. Our method may in turn provide an objective measure for evaluations of individuals with ASD. LAY SUMMARY: People with autism spectrum disorder (ASD) demonstrate atypical visual attention to social stimuli. However, it remains largely unclear how they perceive the world from a first-person perspective. In this study, we employed a deep learning approach to analyze a unique dataset of photos taken by people with and without ASD. Our computer modeling was not only able to discern which photos were taken by individuals with ASD, outperforming ASD experts, but importantly, it revealed critical features that led to successful discrimination, revealing aspects of atypical visual attention in ASD from their first-person perspective.", "gemini": "Những người mắc chứng rối loạn phổ tự kỷ (ASD) biểu hiện sự chú ý không điển hình đối với các kích thích xã hội và ánh nhìn bất thường khi xem hình ảnh về thế giới vật lý. Tuy nhiên, không biết họ cảm nhận thế giới như thế nào từ góc nhìn của người thứ nhất. Trong nghiên cứu này, chúng tôi đã sử dụng máy học để phân loại ảnh chụp ở ba danh mục khác nhau (mọi người, trong nhà và ngoài trời) là ảnh do những người mắc chứng ASD chụp hoặc do những người đồng cấp không mắc chứng ASD chụp. Trình phân loại của chúng tôi phân biệt hiệu quả các bức ảnh từ cả ba danh mục, nhưng đặc biệt thành công trong việc phân loại ảnh của những người mắc chứng ASD với độ chính xác >80%. Điều quan trọng là hình dung mô hình của chúng tôi đã tiết lộ các đặc điểm quan trọng dẫn đến sự phân biệt thành công và cho thấy mô hình của chúng tôi đã áp dụng một chiến lược tương tự như chiến lược của các chuyên gia về ASD. Hơn nữa, lần đầu tiên chúng tôi chỉ ra rằng những bức ảnh do những người mắc chứng ASD chụp có ít đối tượng nổi bật hơn, đặc biệt là ở trường thị giác trung tâm. Đáng chú ý, mô hình của chúng tôi vượt trội hơn phân loại các bức ảnh này của các chuyên gia về ASD. Cùng nhau, chúng tôi trình bày một phương pháp mới và hiệu quả có khả năng phân biệt các bức ảnh do những người mắc chứng ASD chụp và tiết lộ sự chú ý thị giác bất thường ở ASD từ góc nhìn của người thứ nhất độc đáo. Phương pháp của chúng tôi lần lượt có thể cung cấp một biện pháp khách quan để đánh giá những người mắc chứng ASD. TÓM TẮT NGHIÊN CỨU: Những người mắc chứng rối loạn phổ tự kỷ (ASD) thể hiện sự chú ý thị giác không điển hình đối với các kích thích xã hội. Tuy nhiên, phần lớn vẫn chưa rõ họ cảm nhận thế giới như thế nào từ góc nhìn của người thứ nhất. Trong nghiên cứu này, chúng tôi đã sử dụng phương pháp học sâu để phân tích một tập dữ liệu ảnh độc đáo do những người mắc và không mắc chứng ASD chụp. Mô hình máy tính của chúng tôi không chỉ có thể phân biệt được bức ảnh nào do những người mắc chứng ASD chụp, vượt trội hơn các chuyên gia về ASD, mà quan trọng hơn là nó còn tiết lộ các đặc điểm quan trọng dẫn đến sự phân biệt thành công, tiết lộ các khía cạnh của sự chú ý thị giác không điển hình ở ASD từ góc nhìn của người thứ nhất của họ."} +{"idx": 1870334, "en": "Renal cell carcinoma (RCC) is rare in children but is the most common renal tumor in adults. Pediatric RCC has different clinical characteristics, histopathology, and treatment compared with adult disease. Databases were reviewed from inception to February 2020, identifying 32 publications pertaining to 350 patients under 27 years. Surgery is the cornerstone for cure in localized RCC. Lymph node dissection remains controversial. Conventional radiotherapy has no curative role in RCC; similarly, conventional chemotherapy has not proven to be effective in large cohorts. Pediatric metastatic RCC has a poor outlook. There are no published prospective studies demonstrating which adjuvant therapy could improve outcome. Sunitinib, a tyrosine kinase inhibitor, is recommended in this group despite limited evidence. This review provides an overview for pediatric RCC, including the evolving role of precision medicine.", "gemini": "Ung thư biểu mô tế bào thận (RCC) hiếm gặp ở trẻ em nhưng là khối u thận phổ biến nhất ở người lớn. RCC ở trẻ em có đặc điểm lâm sàng, mô bệnh học và điều trị khác so với bệnh ở người lớn. Các cơ sở dữ liệu đã được xem xét từ khi thành lập đến tháng 2 năm 2020, xác định 32 ấn phẩm liên quan đến 350 bệnh nhân dưới 27 tuổi. Phẫu thuật là nền tảng để chữa khỏi RCC tại chỗ. Phẫu tích hạch vẫn còn gây tranh cãi. Xạ trị thông thường không có vai trò chữa khỏi RCC; tương tự, hóa trị thông thường chưa được chứng minh là có hiệu quả trên các nhóm lớn. RCC di căn ở trẻ em có tiên lượng kém. Không có nghiên cứu triển vọng nào được công bố chứng minh liệu pháp bổ trợ nào có thể cải thiện kết quả. Sunitinib, một chất ức chế tyrosine kinase, được khuyến cáo trong nhóm này mặc dù có bằng chứng hạn chế. Tổng quan này cung cấp cái nhìn tổng quan về RCC ở trẻ em, bao gồm cả vai trò đang phát triển của y học chính xác."} +{"idx": 1870335, "en": "BACKGROUND Childhood obesity continues to be a critical health concern in the United States. Nonetheless, interventions that focus on delivering verbal lessons about food and health to children in preschool classrooms have had only modest effects. OBJECTIVES The present study examines the relative effectiveness of showing vs telling children about food to promote healthy eating, with a focus on unfamiliar foods and vegetables. METHODS Three- to six-year-old children (n = 71) were tested in a laboratory study in which they watched videos of two people eating apple-broccoli puree. One person took five bites of the food; the other said they liked the food. RESULTS Children did not differentiate between the food they saw someone eat and the food they heard someone talk about. Children's food intake was negatively associated with parent reports of children's eating behavior on the Food Fussiness subscale of the Child Eating Behavior Questionnaire. We found similar patterns in an analogous toy task. In an unfamiliar object task, children selected the action demonstration as the right way to use the object. CONCLUSIONS We find no evidence that action vs verbal testimony is more persuasive in guiding children's food choices, but action testimony may be persuasive in other domains. The associations between children's food intake and pickiness provide growing evidence of alignment between parent assessments of their children's typical eating behavior and children's food choices in laboratory studies.", "gemini": "BỐI CẢNH Béo phì ở trẻ em tiếp tục là mối quan tâm sức khỏe quan trọng ở Hoa Kỳ. Tuy nhiên, các biện pháp can thiệp tập trung vào việc truyền đạt các bài học bằng lời về thực phẩm và sức khỏe cho trẻ em trong các lớp học mẫu giáo chỉ có tác dụng khiêm tốn. MỤC TIÊU Nghiên cứu hiện tại xem xét hiệu quả tương đối của việc cho trẻ xem so với việc nói với trẻ về thực phẩm để thúc đẩy ăn uống lành mạnh, tập trung vào các loại thực phẩm và rau không quen thuộc. PHƯƠNG PHÁP Trẻ em từ ba đến sáu tuổi (n = 71) đã được kiểm tra trong một nghiên cứu trong phòng thí nghiệm, trong đó chúng xem video về hai người ăn cháo táo-súp lơ. Một người ăn năm miếng thức ăn; người kia nói rằng họ thích đồ ăn. KẾT QUẢ Trẻ em không phân biệt được giữa thức ăn chúng thấy ai đó ăn và thức ăn chúng nghe ai đó nói. Lượng thức ăn của trẻ có liên quan tiêu cực đến báo cáo của cha mẹ về hành vi ăn uống của trẻ trên thang phụ Food Fussiness của Bản câu hỏi về hành vi ăn uống của trẻ. Chúng tôi tìm thấy các mô hình tương tự trong một nhiệm vụ đồ chơi tương tự. Trong một nhiệm vụ đối tượng không quen thuộc, trẻ em đã chọn hành động trình diễn là cách sử dụng đúng đối tượng. KẾT LUẬN Chúng tôi không tìm thấy bằng chứng nào cho thấy hành động so với lời chứng thực bằng lời thuyết phục hơn trong việc hướng dẫn trẻ lựa chọn thực phẩm, nhưng lời chứng thực hành động có thể thuyết phục trong các lĩnh vực khác. Mối liên hệ giữa lượng thức ăn của trẻ em và sự kén ăn cung cấp bằng chứng ngày càng tăng về sự thống nhất giữa đánh giá của cha mẹ về hành vi ăn uống điển hình của con mình và lựa chọn thực phẩm của trẻ trong các nghiên cứu trong phòng thí nghiệm."} +{"idx": 1870336, "en": "Very little is known about how metabolic health status, insulin resistance or metabolic challenges modulate the endocannabinoid (eCB) or polyunsaturated fatty acid (PUFA)-derived oxylipin (OxL) lipid classes. To address these questions, plasma eCB and OxL concentrations were determined at rest, 10 and 20 min during an acute exercise bout (30 min total, ~45% of preintervention V̇O2peak , ~63 W), and following 20 min recovery in overnight-fasted sedentary, obese, insulin-resistant women under controlled diet conditions. We hypothesized that increased fitness and insulin sensitivity following a ~14-week training and weight loss intervention would lead to significant changes in lipid signatures using an identical acute exercise protocol to preintervention. In the first 10 min of exercise, concentrations of a suite of OxL diols and hydroxyeicosatetraenoic acid (HETE) metabolites dropped significantly. There was no increase in 12,13-DiHOME, previously reported to increase with exercise and proposed to activate muscle fatty acid uptake and tissue metabolism. Following weight loss intervention, exercise-associated reductions were more pronounced for several linoleate and alpha-linolenate metabolites including DiHOMEs, DiHODEs, KODEs, and EpODEs, and fasting concentrations of 9,10-DiHODE, 12,13-DiHODE, and 9,10-DiHOME were reduced. These findings suggest that improved metabolic health modifies soluble epoxide hydrolase, cytochrome P450 epoxygenase (CYP), and lipoxygenase (LOX) systems. Acute exercise led to reductions for most eCB metabolites, with no evidence for concentration increases even at recovery. It is proposed that during submaximal aerobic exercise, nonoxidative fates of long-chain saturated, monounsaturated, and PUFAs are attenuated in tissues that are important contributors to the blood OxL and eCB pools.", "gemini": "Người ta biết rất ít về cách trạng thái sức khỏe trao đổi chất, tình trạng kháng insulin hoặc các thách thức trao đổi chất điều chỉnh các lớp lipid oxylipin (OxL) có nguồn gốc từ endocannabinoid (eCB) hoặc axit béo không bão hòa đa (PUFA). Để giải quyết những câu hỏi này, nồng độ eCB và OxL trong huyết tương đã được xác định khi nghỉ ngơi, 10 và 20 phút trong một đợt tập thể dục cấp tính (tổng cộng 30 phút, ~ 45% V̇O2peak trước can thiệp, ~ 63 W), và sau 20 phút phục hồi ở những phụ nữ béo phì, ít vận động, kháng insulin đã nhịn ăn qua đêm trong điều kiện chế độ ăn được kiểm soát. Chúng tôi đưa ra giả thuyết rằng việc tăng cường thể lực và độ nhạy insulin sau can thiệp giảm cân và tập luyện trong ~ 14 tuần sẽ dẫn đến những thay đổi đáng kể trong các đặc điểm lipid bằng cách sử dụng một phác đồ tập thể dục cấp tính giống hệt với trước khi can thiệp. Trong 10 phút đầu tiên tập thể dục, nồng độ của một loạt các chất chuyển hóa OxL diol và axit hydroxyeicosatetraenoic (HETE) giảm đáng kể. Không có sự gia tăng 12,13-DiHOME, trước đây được báo cáo là tăng lên khi tập thể dục và được đề xuất để kích hoạt quá trình hấp thụ axit béo của cơ và quá trình trao đổi chất của mô. Sau khi can thiệp giảm cân, sự giảm liên quan đến tập thể dục rõ rệt hơn đối với một số chất chuyển hóa linoleat và alpha-linolenat bao gồm DiHOME, DiHODE, KODE và EpODE, và nồng độ khi đói của 9,10-DiHODE, 12,13-DiHODE và 9,10-DiHOME giảm. Những phát hiện này cho thấy rằng sức khỏe trao đổi chất được cải thiện sẽ làm thay đổi hệ thống epoxide hydrolase hòa tan, cytochrome P450 epoxygenase (CYP) và lipoxygenase (LOX). Tập thể dục cấp tính dẫn đến sự giảm đối với hầu hết các chất chuyển hóa eCB, không có bằng chứng về sự gia tăng nồng độ ngay cả khi phục hồi. Người ta đề xuất rằng trong quá trình tập thể dục hiếu khí dưới mức tối đa, số phận không oxy hóa của các axit béo không bão hòa đơn, bão hòa mạch dài và PUFA bị suy yếu trong các mô đóng góp quan trọng vào các nhóm OxL và eCB trong máu."} +{"idx": 1870337, "en": "Functional supramolecular micelles containing self-complementary multiple hydrogen bonding adenine groups (A-PPG) can spontaneously self-assemble into stable nanosized micelles in aqueous solution. These micelles can be used to selectively deliver anticancer drugs to cancer cells and effectively promote tumor cell death via apoptosis, without harming normal cells. The drug-loaded micelles exhibit tunable drug-loading capacity and rapid pH-triggered drug release under acidic conditions, as well as a high drug-entrapment stability in serum-rich media due to the reversible hydrogen-bonded adenine-adenine interactions within the micellar interior; these properties are critical to achieving effective chemotherapeutic drug delivery and controlled drug release. In vitro assays show that the drug-loaded micelles exert significant cytotoxic effects on cancer cells, with minimal effects on normal cells under physiological conditions. Cytotoxicity assays using A-PPG micelles loaded with different anticancer drugs confirm these effects. Importantly, cellular internalization and flow cytometric analyses demonstrate that the adenine moieties within A-PPG micelles significantly increase selective endocytic uptake of the supramolecular micelles by cancer cells, which in turn induce apoptotic cell death and substantially enhance the response to chemotherapy. Thus, A-PPG micelles can improve the safety and efficacy of cancer chemotherapy.", "gemini": "Các micelle siêu phân tử chức năng chứa các nhóm adenin liên kết hydro đa bù trừ tự thân (A-PPG) có thể tự lắp ráp thành các micelle kích thước nano ổn định trong dung dịch nước. Các micelle này có thể được sử dụng để đưa thuốc chống ung thư một cách chọn lọc vào các tế bào ung thư và thúc đẩy hiệu quả quá trình chết tế bào khối u thông qua quá trình apoptosis, mà không gây hại cho các tế bào bình thường. Các micelle chứa thuốc thể hiện khả năng tải thuốc có thể điều chỉnh và giải phóng thuốc nhanh chóng khi kích thích pH trong điều kiện axit, cũng như độ ổn định giữ thuốc cao trong môi trường giàu huyết thanh do các tương tác adenin-adenin liên kết hydro có thể đảo ngược bên trong micelle; các đặc tính này rất quan trọng để đạt được hiệu quả đưa thuốc hóa trị và giải phóng thuốc có kiểm soát. Các xét nghiệm trong ống nghiệm cho thấy các micelle chứa thuốc gây ra tác dụng độc tế bào đáng kể đối với các tế bào ung thư, với tác động tối thiểu đối với các tế bào bình thường trong điều kiện sinh lý. Các xét nghiệm độc tế bào sử dụng micelle A-PPG chứa các loại thuốc chống ung thư khác nhau xác nhận những tác động này. Quan trọng hơn, quá trình nội bào tế bào và phân tích tế bào dòng chảy chứng minh rằng các nhóm adenin trong micelle A-PPG làm tăng đáng kể quá trình hấp thụ nội bào chọn lọc các micelle siêu phân tử bởi các tế bào ung thư, từ đó gây ra quá trình chết tế bào theo chương trình apoptosis và tăng cường đáng kể đáp ứng với hóa trị liệu. Do đó, các micelle A-PPG có thể cải thiện tính an toàn và hiệu quả của hóa trị liệu ung thư."} +{"idx": 1870338, "en": "To avoid excessive tissue removal and collateral damage, the high-power density laser is apt for dental surgery also need to have high precision. For high-precision dental surgery with minimal tissue damage, the present work frames a method to predict laser ablation profile based on surface morphology and chemical composition of dentin. The surface morphology and chemical composition were studied on different dentin samples using scanning electron microscope (SEM) and Energy Dispersive X-ray Analysis (EDAX), respectively. The key laser ablation parameters (ω0 , Deff , and Fth ) were determined by laser irradiation study using 800 nm, Ti:Sapphire femtosecond laser at processing condition of 100 fs, 10 kHz and 10 mm/s. The dentin samples show a strong linear correlation between physiochemical characteristics and laser ablation parameters. The surface morphology exhibits a negative linear correlation with threshold fluence, whereas the converse is true for chemical composition. The laser ablation parameters of a random dentin sample are derived from the knowledge of linearity data. From the obtained laser ablation parameters, the complete theoretical ablation profile is constructed and validated with experimental ablation profile. Even though the surface morphology of dentin shows high linearity, the concentration of Ca and P can be used as the most feasible probe in clinical settings. Furthermore, the laser ablation rate and ablation efficiency are predicted by the method to optimize the laser processing condition for any specific teeth. The versatility of the method overcomes the problem of heterogeneity on various teeth and simplifies the method of finding optimal laser processing condition for immaculate laser surgery.", "gemini": "Để tránh loại bỏ mô quá mức và tổn thương bên, tia laser mật độ năng lượng cao thích hợp cho phẫu thuật nha khoa cũng cần có độ chính xác cao. Đối với phẫu thuật nha khoa có độ chính xác cao với tổn thương mô tối thiểu, công trình hiện tại lập một phương pháp để dự đoán cấu hình phá hủy laser dựa trên hình thái bề mặt và thành phần hóa học của ngà răng. Hình thái bề mặt và thành phần hóa học đã được nghiên cứu trên các mẫu ngà răng khác nhau bằng kính hiển vi điện tử quét (SEM) và Phân tích tia X phân tán năng lượng (EDAX). Các thông số phá hủy laser chính (ω0, Deff và Fth) được xác định bằng nghiên cứu chiếu xạ laser sử dụng laser femtosecond Ti: Sapphire 800 nm ở điều kiện xử lý là 100 fs, 10 kHz và 10 mm/s. Các mẫu ngà răng cho thấy mối tương quan tuyến tính mạnh giữa các đặc tính vật lý hóa học và các thông số phá hủy laser. Hình thái bề mặt thể hiện mối tương quan tuyến tính âm với ngưỡng phát sáng, trong khi thành phần hóa học thì ngược lại. Các thông số phá hủy laser của một mẫu ngà răng ngẫu nhiên được lấy từ dữ liệu tuyến tính. Từ các thông số phá hủy laser thu được, cấu hình phá hủy lý thuyết hoàn chỉnh được xây dựng và xác thực bằng cấu hình phá hủy thực nghiệm. Mặc dù hình thái bề mặt của ngà răng cho thấy tính tuyến tính cao, nhưng nồng độ Ca và P có thể được sử dụng làm đầu dò khả thi nhất trong các thiết lập lâm sàng. Hơn nữa, tốc độ phá hủy laser và hiệu suất phá hủy được phương pháp dự đoán để tối ưu hóa điều kiện xử lý laser cho bất kỳ loại răng cụ thể nào. Tính linh hoạt của phương pháp khắc phục được vấn đề không đồng nhất trên các răng khác nhau và đơn giản hóa phương pháp tìm điều kiện xử lý laser tối ưu cho phẫu thuật laser hoàn hảo."} +{"idx": 1870339, "en": "BACKGROUND Thoracoscopic surgery of congenital diaphragmatic hernia (CDH) is connected with a higher incidence of recurrence than open repair is. This is usually caused by the dehiscence of sutures in the lateral part of the defect. This area is characterized by increased tension on proximate tissues and difficult thoracoscopic suturing. For more effective repair, the authors adopted a variant of percutaneous internal ring suturing (PIRS) technique. OBJECTIVES To present and evaluate the efficacy of the PIRS technique for the repair of CDH. MATERIAL AND METHODS The study is based on retrospective analysis of the medical data of patients with CDH treated in the Department of Pediatric Surgery of the Jagiellonian University Medical College (Kraków, Poland) from January 2013 to July 2019. The PIRS technique was applied when thoracoscopic repair under acceptable tension appeared impossible. RESULTS Fifty-one patients were identified. Of these, 11 children died before surgery and 1 after, leaving 39 (76%) who were operated on and survived. Thoracoscopy was used in 27 cases (69%), with 3 conversions. The recurrence rate in patients who underwent thoracoscopic closure of the defect with intermittent sutures was 27% (3 out of 11 children), while in the group that underwent thoracoscopic repair with the additional use of percutaneous suturing, the recurrence rate was 6.25% (1 out of 16 patients). The follow-up periods ranged from 4 months to 6 years. CONCLUSIONS The applied PIRS technique permits safe and effective closure of intermediate-size diaphragmatic defects under acceptable tension. The method is feasible and can be listed among countermeasures against recurrence.", "gemini": "BỐI CẢNH Phẫu thuật nội soi lồng ngực thoát vị hoành bẩm sinh (CDH) có liên quan đến tỷ lệ tái phát cao hơn so với phẫu thuật mở. Nguyên nhân thường là do vết khâu bị nứt ở phần bên của khuyết tật. Khu vực này được đặc trưng bởi sự căng thẳng gia tăng trên các mô gần kề và khó khâu nội soi lồng ngực. Để sửa chữa hiệu quả hơn, các tác giả đã áp dụng một biến thể của kỹ thuật khâu vòng trong da (PIRS). MỤC TIÊU Trình bày và đánh giá hiệu quả của kỹ thuật PIRS trong việc sửa chữa CDH. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu dựa trên phân tích hồi cứu dữ liệu y tế của bệnh nhân bị CDH được điều trị tại Khoa Phẫu thuật Nhi của Đại học Y Jagiellonian (Kraków, Ba Lan) từ tháng 1 năm 2013 đến tháng 7 năm 2019. Kỹ thuật PIRS được áp dụng khi không thể tiến hành sửa chữa nội soi lồng ngực dưới sức căng chấp nhận được. KẾT QUẢ Năm mươi mốt bệnh nhân đã được xác định. Trong số đó, 11 trẻ tử vong trước khi phẫu thuật và 1 trẻ tử vong sau phẫu thuật, còn 39 trẻ (76%) được phẫu thuật và sống sót. Nội soi lồng ngực được sử dụng trong 27 trường hợp (69%), với 3 lần chuyển đổi. Tỷ lệ tái phát ở những bệnh nhân được khâu đóng khuyết tật nội soi lồng ngực bằng chỉ khâu gián đoạn là 27% (3/11 trẻ), trong khi ở nhóm được sửa chữa nội soi lồng ngực với việc sử dụng thêm chỉ khâu qua da, tỷ lệ tái phát là 6,25% (1/16 bệnh nhân). Thời gian theo dõi dao động từ 4 tháng đến 6 năm. KẾT LUẬN Kỹ thuật PIRS được áp dụng cho phép đóng an toàn và hiệu quả các khuyết tật cơ hoành có kích thước trung bình dưới sức căng chấp nhận được. Phương pháp này khả thi và có thể được liệt kê vào các biện pháp đối phó với tái phát."} +{"idx": 1870340, "en": "Background Functional underpinnings of cognitive control deficits in unbiased samples (i.e., all comers) of patients with psychotic spectrum disorders (PSD) remain actively debated. While many studies suggest hypofrontality in the lateral prefrontal cortex (PFC) and greater deficits during proactive relative to reactive control, few have examined the full hemodynamic response. Methods Patients with PSD (n = 154) and healthy controls (n = 65) performed the AX continuous performance task (AX-CPT) during rapid (460 ms) functional neuroimaging and underwent full clinical characterization. Results Behavioural results indicated generalized cognitive deficits (slower and less accurate) across proactive and reactive control conditions in patients with PSD relative to healthy controls. We observed a delayed/prolonged neural response in the left dorsolateral PFC, the sensorimotor cortex and the superior parietal lobe during proactive control for patients with PSD. These proactive hemodynamic abnormalities were better explained by negative rather than by positive symptoms or by traditional diagnoses according to the Diagnostic and Statistical Manual of Mental Disorders Fourth Edition, Text Revision (DSM-IV-TR), with subsequent simulations unequivocally demonstrating how these abnormalities could be erroneously interpreted as hypoactivation. Conversely, true hypoactivity, unassociated with clinical symptoms or DSM-IV-TR diagnoses, was observed within the ventrolateral PFC during reactive control. Limitations In spite of guidance for AX-CPT use in neuroimaging studies, one-third of patients with PSD could not perform the task above chance and were more clinically impaired. Conclusion Current findings question the utility of the AX-CPT for neuroimaging-based appraisal of cognitive control across the full spectrum of patients with PSD. Previously reported lateral PFC \"hypoactivity\" during proactive control may be more indicative of a delayed/prolonged neural response, important for rehabilitative purposes. Negative symptoms may better explain certain behavioural and hemodynamic abnormalities in patients with PSD relative to DSM-IV-TR diagnoses.", "gemini": "Bối cảnh Nền tảng chức năng của tình trạng thiếu hụt kiểm soát nhận thức trong các mẫu không thiên vị (tức là tất cả những người đến) của bệnh nhân mắc các rối loạn phổ loạn thần (PSD) vẫn đang được tranh luận tích cực. Trong khi nhiều nghiên cứu chỉ ra tình trạng giảm hoạt động ở vỏ não trước trán bên (PFC) và tình trạng thiếu hụt lớn hơn trong quá trình kiểm soát chủ động so với phản ứng, thì rất ít nghiên cứu kiểm tra phản ứng huyết động học đầy đủ. Phương pháp Bệnh nhân mắc PSD (n = 154) và nhóm đối chứng khỏe mạnh (n = 65) đã thực hiện nhiệm vụ kiểm tra hiệu suất liên tục AX (AX-CPT) trong quá trình chụp ảnh thần kinh chức năng nhanh (460 ms) và trải qua quá trình mô tả lâm sàng đầy đủ. Kết quả Kết quả hành vi chỉ ra tình trạng thiếu hụt nhận thức tổng quát (chậm hơn và ít chính xác hơn) trong các điều kiện kiểm soát chủ động và phản ứng ở bệnh nhân mắc PSD so với nhóm đối chứng khỏe mạnh. Chúng tôi quan sát thấy phản ứng thần kinh chậm trễ/kéo dài ở PFC lưng bên trái, vỏ não vận động cảm giác và thùy đỉnh trên trong quá trình kiểm soát chủ động ở bệnh nhân mắc PSD. Những bất thường huyết động học chủ động này được giải thích tốt hơn bằng các triệu chứng tiêu cực hơn là các triệu chứng tích cực hoặc bằng các chẩn đoán truyền thống theo Sổ tay chẩn đoán và thống kê về các rối loạn tâm thần, Sửa đổi văn bản lần thứ tư (DSM-IV-TR), với các mô phỏng tiếp theo chứng minh rõ ràng cách giải thích sai những bất thường này như là tình trạng giảm hoạt động. Ngược lại, tình trạng giảm hoạt động thực sự, không liên quan đến các triệu chứng lâm sàng hoặc chẩn đoán DSM-IV-TR, đã được quan sát thấy trong PFC bên ngoài trong quá trình kiểm soát phản ứng. Hạn chế Mặc dù có hướng dẫn sử dụng AX-CPT trong các nghiên cứu chụp ảnh thần kinh, nhưng một phần ba bệnh nhân mắc PSD không thể thực hiện nhiệm vụ trên mức ngẫu nhiên và bị suy giảm lâm sàng nhiều hơn. Kết luận Những phát hiện hiện tại đặt câu hỏi về tiện ích của AX-CPT để đánh giá kiểm soát nhận thức dựa trên chụp ảnh thần kinh trên toàn bộ phổ bệnh nhân mắc PSD. \"Giảm hoạt động\" PFC bên đã báo cáo trước đây trong quá trình kiểm soát chủ động có thể chỉ ra nhiều hơn về phản ứng thần kinh chậm trễ/kéo dài, điều quan trọng cho mục đích phục hồi chức năng. Các triệu chứng tiêu cực có thể giải thích tốt hơn một số bất thường về hành vi và huyết động học ở bệnh nhân mắc PSD so với chẩn đoán DSM-IV-TR."} +{"idx": 1870341, "en": "INTRODUCTION This paper is the first update of the second edition of the rapid living systematic review on the latest scientific literature informing rehabilitation of patients with COVID-19 and/or describing consequences of the disease and its treatment, as they relate to limitations in functioning of rehabilitation interest. The aim of this study was to report data of a systematic search performed on papers published in July 2020. EVIDENCE ACQUISITION The methodology described in the second edition of the rapid living systematic review was applied to search eligible papers included in the databases between July 1, 2020 and July 31, 2020. EVIDENCE SYNTHESIS Eight-hundred-ninety-two papers were identified through database searching (after removal of duplicates); of these, only 23 studies were included. According to OCEBM 2011 Levels of Evidence Table, they were level 3 in 30.5% cases and level 4 in 69.5%. No RCT was found. Nineteen papers studied COVID-19 patients, assessed in the acute (10 studies), post-acute (8 studies) and chronic phase (one study). Four studies reported data on the impact of COVID-19 on subjects with pre-existing health conditions. CONCLUSIONS The current literature production still focuses more on describing all the possible aspects and complications of the pathology than on interventions or new organization models to deal with it. Albeit evidence on handling COVID-19 from a rehabilitative point of view is improving each month, further studies are still mandatory to report the role of rehabilitation in this scenario.", "gemini": "GIỚI THIỆU Bài báo này là bản cập nhật đầu tiên của ấn bản thứ hai của bản tổng quan hệ thống cập nhật nhanh về các tài liệu khoa học mới nhất cung cấp thông tin về phục hồi chức năng cho bệnh nhân mắc COVID-19 và/hoặc mô tả hậu quả của bệnh và cách điều trị, vì chúng liên quan đến những hạn chế trong chức năng phục hồi chức năng quan tâm. Mục đích của nghiên cứu này là báo cáo dữ liệu của một tìm kiếm hệ thống được thực hiện trên các bài báo được công bố vào tháng 7 năm 2020. THU THẬP BẰNG CHỨNG Phương pháp được mô tả trong ấn bản thứ hai của bản tổng quan hệ thống cập nhật nhanh đã được áp dụng để tìm kiếm các bài báo đủ điều kiện có trong cơ sở dữ liệu từ ngày 1 tháng 7 năm 2020 đến ngày 31 tháng 7 năm 2020. TỔNG HỢP BẰNG CHỨNG Tám trăm chín mươi hai bài báo đã được xác định thông qua tìm kiếm cơ sở dữ liệu (sau khi loại bỏ các bản trùng lặp); trong số đó, chỉ có 23 nghiên cứu được đưa vào. Theo Bảng mức độ bằng chứng OCEBM 2011, chúng là mức 3 trong 30,5% trường hợp và mức 4 trong 69,5%. Không tìm thấy RCT nào. Mười chín bài báo nghiên cứu bệnh nhân COVID-19, được đánh giá ở giai đoạn cấp tính (10 nghiên cứu), hậu cấp tính (8 nghiên cứu) và giai đoạn mãn tính (một nghiên cứu). Bốn nghiên cứu báo cáo dữ liệu về tác động của COVID-19 đối với những đối tượng có tình trạng sức khỏe trước đó. KẾT LUẬN Sản phẩm tài liệu hiện tại vẫn tập trung nhiều hơn vào việc mô tả tất cả các khía cạnh và biến chứng có thể xảy ra của bệnh lý hơn là các biện pháp can thiệp hoặc mô hình tổ chức mới để giải quyết vấn đề này. Mặc dù bằng chứng về việc xử lý COVID-19 theo quan điểm phục hồi chức năng đang được cải thiện từng tháng, nhưng các nghiên cứu sâu hơn vẫn bắt buộc để báo cáo vai trò của phục hồi chức năng trong kịch bản này."} +{"idx": 1870342, "en": "Assessment of mandibular mobility is an integral part of many dental examinations. Yet, information on the maximum range of mandibular motion in large groups of patients with temporomandibular disorders (TMDs) is limited. 
Therefore, we analyzed the corresponding data of 500 TMD patients who had presented at the University Center for Dental Medicine Basel. All measurements had been carried out by one examiner. The average maximal values for unassisted jaw opening, protrusion, and movement to the right and left were 49.3 mm (SD: 9.1), 8.8 mm (SD: 2.3), 9.4 mm (SD: 2.5), and 11.8 mm (SD: 3.1), respectively. Since activities such as chewing, talking, oral hygiene or even yawning require neither very wide openings nor extensive lateral or protrusive excursions, we would like to propose the following general threshold values for impaired mandibular function: maximum interincisal distance < 30 mm; maximum laterotrusion as well as maximum protrusion < 5 mm.
 These cut-off values, which are lower than those traditionally suggested in the dental literature, appear to be sufficient to carry out functionally undisturbed mandibular movements. By lowering the traditionally higher thresholds, the spectrum of anatomical and functional variability is increased. In this way, patients and non-patients may be protected against medicalization, overdiagnosis, and overtreatment.", "gemini": "Đánh giá khả năng vận động của xương hàm dưới là một phần không thể thiếu trong nhiều cuộc kiểm tra nha khoa. Tuy nhiên, thông tin về phạm vi chuyển động tối đa của xương hàm dưới ở các nhóm bệnh nhân lớn mắc chứng rối loạn khớp thái dương hàm (TMD) còn hạn chế. Do đó, chúng tôi đã phân tích dữ liệu tương ứng của 500 bệnh nhân TMD đã đến Trung tâm Nha khoa Đại học Basel. Tất cả các phép đo đều được thực hiện bởi một giám định viên. Các giá trị cực đại trung bình đối với việc mở hàm không hỗ trợ, nhô ra và chuyển động sang phải và trái lần lượt là 49,3 mm (SD: 9,1), 8,8 mm (SD: 2,3), 9,4 mm (SD: 2,5) và 11,8 mm (SD: 3,1). Vì các hoạt động như nhai, nói, vệ sinh răng miệng hoặc thậm chí ngáp không đòi hỏi phải mở miệng quá rộng hoặc các chuyến đi bên hoặc nhô ra quá nhiều, chúng tôi muốn đề xuất các giá trị ngưỡng chung sau đối với chức năng xương hàm dưới bị suy yếu: khoảng cách liên răng cửa tối đa <30 mm; độ nhô ra bên tối đa cũng như độ nhô ra tối đa <5 mm. Các giá trị cắt giảm này, thấp hơn các giá trị được đề xuất theo truyền thống trong tài liệu nha khoa, dường như đủ để thực hiện các chuyển động của xương hàm dưới không bị ảnh hưởng về chức năng. Bằng cách hạ thấp các ngưỡng cao hơn theo truyền thống, phổ biến dạng giải phẫu và chức năng được tăng lên. Theo cách này, bệnh nhân và người không phải bệnh nhân có thể được bảo vệ khỏi việc y tế hóa, chẩn đoán quá mức và điều trị quá mức."} +{"idx": 1870343, "en": "Since the discovery of LiNiO2 several decades ago, a new era of electric vehicles demanding high-energy-density lithium-ion batteries (LIBs) has recently rebooted the interest in this cathode material to eliminate the dependence on expensive and scarcely available cobalt. However, LiNiO2 has been plagued by cycle instability, thermal instability, and air instability. We present here an exploration of the mutual interaction of magnesium and copper in stabilizing the cobalt-free LiNiO2 cathode. Although Mg doping is beneficial for the robustness of the bulk structure of LiNiO2, surface characterization results of Mg-doped LiNiO2 implies the need for further surface protection. To that end, we have incorporated Cu in addition to Mg in that Cu stabilizes the surface of Mg-doped LiNiO2 by forming a protective stable surface layer without harming the bulk. Notable variations of the surface residual lithium composition (LiLi2CO3/LiLiOH) along with the incorporation of stabilizers are also discussed. The harmony between Mg and Cu with as little as 0.5 atom % Mg and 0.3 atom % Cu significantly enhances the specific energy and cycle life of LiNiO2. This study demonstrates how the co-incorporation of optimal dopants can help stabilize both the bulk and surface and provides new insights toward developing cobalt-free layered oxide cathodes for high-energy-density LIBs.", "gemini": "Kể từ khi phát hiện ra LiNiO2 cách đây vài thập kỷ, kỷ nguyên mới của xe điện đòi hỏi pin lithium-ion (LIB) có mật độ năng lượng cao đã khởi động lại sự quan tâm đến vật liệu catốt này để loại bỏ sự phụ thuộc vào coban đắt tiền và khan hiếm. Tuy nhiên, LiNiO2 đã bị ảnh hưởng bởi sự không ổn định của chu kỳ, sự không ổn định nhiệt và sự không ổn định của không khí. Chúng tôi trình bày ở đây một cuộc khám phá về sự tương tác lẫn nhau của magiê và đồng trong việc ổn định catốt LiNiO2 không chứa coban. Mặc dù việc bổ sung Mg có lợi cho sự chắc chắn của cấu trúc khối của LiNiO2, nhưng kết quả đặc tính bề mặt của LiNiO2 có bổ sung Mg cho thấy cần phải bảo vệ bề mặt nhiều hơn nữa. Để đạt được mục đích đó, chúng tôi đã kết hợp Cu ngoài Mg trong đó Cu ổn định bề mặt của LiNiO2 có bổ sung Mg bằng cách hình thành một lớp bề mặt ổn định bảo vệ mà không gây hại cho khối lượng lớn. Các biến thể đáng chú ý của thành phần lithium dư trên bề mặt (LiLi2CO3/LiLiOH) cùng với việc kết hợp các chất ổn định cũng được thảo luận. Sự hài hòa giữa Mg và Cu với ít nhất 0,5% nguyên tử Mg và 0,3% nguyên tử Cu làm tăng đáng kể năng lượng riêng và tuổi thọ chu kỳ của LiNiO2. Nghiên cứu này chứng minh cách kết hợp đồng thời các chất bổ sung tối ưu có thể giúp ổn định cả khối lượng lớn và bề mặt và cung cấp những hiểu biết mới hướng tới việc phát triển các catốt oxit nhiều lớp không chứa coban cho LIB có mật độ năng lượng cao."} +{"idx": 1870344, "en": "This article reports a nanoporous silicon (Si) thin-film-based high-performance and low-power hydrogen (H2) sensor fabricated by metal-assisted chemical etching (MaCE). The nanoporous Si thin film treated with Pd-based MaCE showed improvement over a flat Si thin film sensor in H2 response (ΔI/I0 = 4.36% → 12.4% for 0.1% H2). Furthermore, it was verified that the combination of thermal annealing of Pd and subsequent MaCE on the Si thin film synergistically enhances the H2 sensitivity of the sensor by 65 times as compared to the flat Si thin film sensor (ΔI/I0 = 4.36% → 285% for 0.1% H2). This sensor also showed a very low operating power of 1.62 μW. After the thermal treatment, densely packed Pd nanoparticles agglomerate due to dewetting, which results in a higher surface-to-volume ratio by well-defined etched holes, leading to an increase in sensor response.", "gemini": "Bài báo này báo cáo một cảm biến hydro (H2) hiệu suất cao và công suất thấp dựa trên màng mỏng silicon (Si) xốp được chế tạo bằng phương pháp khắc hóa học có hỗ trợ kim loại (MaCE). Màng mỏng Si xốp được xử lý bằng MaCE gốc Pd cho thấy sự cải thiện so với cảm biến màng mỏng Si phẳng về phản ứng H2 (ΔI/I0 = 4,36% → 12,4% đối với 0,1% H2). Hơn nữa, đã được xác minh rằng sự kết hợp giữa ủ nhiệt Pd và MaCE tiếp theo trên màng mỏng Si tăng cường độ nhạy H2 của cảm biến lên 65 lần so với cảm biến màng mỏng Si phẳng (ΔI/I0 = 4,36% → 285% đối với 0,1% H2). Cảm biến này cũng cho thấy công suất hoạt động rất thấp là 1,62 μW. Sau khi xử lý nhiệt, các hạt nano Pd đóng gói dày đặc kết tụ do hiện tượng làm ướt, dẫn đến tỷ lệ diện tích bề mặt trên thể tích cao hơn bằng các lỗ được khắc rõ ràng, dẫn đến tăng phản ứng của cảm biến."} +{"idx": 1870345, "en": "Guanidinium (GA) has been widely used as an additive in solar cells for enhanced performance. However, the size of the guanidinium cation is too large to be incorporated in the cage of the perovskite structure. Instead, GA forms a variety of structures with lead iodide, where its role in the perovskite crystal as well as solar cell devices is unclear. In this study, we demonstrate that GA can be incorporated into the structure of MAPbI3 as (GA)(MA)1-PbI3. From single-crystal X-ray crystallographic refinement, we observe lattice expansion and Pb-I bond elongation with GA incorporation similar to exerting \"negative pressure\", which weakens orbital overlap and widens the band gap from 1.49 to 1.53 eV. We find that the highest percentage of GA that can be incorporated into the 3D MAPbI3 structure is 5.26%, as confirmed by nuclear magnetic resonance. The alloyed (GA)(MA)1-PbI3 exhibits increased PL lifetimes from 154.4 to 266.3 ns after GA incorporation while the Voc of (GA)(MA)1-PbI3 devices enlarges from 1.05 to 1.11 V. High efficiencies in solar cell devices up to 20.38% with a Jsc of 23.55 mA cm-2, Voc of 1.11 V, and FF of 0.78 have been achieved, with stable photovoltaic performance for 900 h in air.", "gemini": "Guanidinium (GA) đã được sử dụng rộng rãi như một chất phụ gia trong pin mặt trời để tăng cường hiệu suất. Tuy nhiên, kích thước của cation guanidinium quá lớn để có thể đưa vào trong lồng của cấu trúc perovskit. Thay vào đó, GA tạo thành nhiều cấu trúc khác nhau với chì iodide, trong đó vai trò của nó trong tinh thể perovskit cũng như các thiết bị pin mặt trời vẫn chưa rõ ràng. Trong nghiên cứu này, chúng tôi chứng minh rằng GA có thể được đưa vào cấu trúc của MAPbI3 dưới dạng (GA)(MA)1-PbI3. Từ tinh chỉnh tinh thể học tia X đơn tinh thể, chúng tôi quan sát thấy sự giãn nở mạng và sự kéo dài liên kết Pb-I với sự kết hợp của GA tương tự như tác động \"áp suất âm\", làm suy yếu sự chồng chéo quỹ đạo và mở rộng khoảng cách dải từ 1,49 đến 1,53 eV. Chúng tôi thấy rằng tỷ lệ phần trăm GA cao nhất có thể được đưa vào cấu trúc MAPbI3 3D là 5,26%, được xác nhận bằng cộng hưởng từ hạt nhân. (GA)(MA)1-PbI3 hợp kim biểu hiện thời gian sống PL tăng từ 154,4 lên 266,3 ns sau khi kết hợp GA trong khi Voc của các thiết bị (GA)(MA)1-PbI3 mở rộng từ 1,05 lên 1,11 V. Hiệu suất cao trong các thiết bị pin mặt trời lên đến 20,38% với Jsc là 23,55 mA cm-2, Voc là 1,11 V và FF là 0,78 đã đạt được, với hiệu suất quang điện ổn định trong 900 giờ trong không khí."} +{"idx": 1870346, "en": "Raman-encoded gold nanoparticles (NPs) have been widely employed as photostable multifunctional probes for sensing, bioimaging, multiplex diagnostics, and surface-enhanced Raman scattering (SERS)-guided tumor therapy. We report a strategy toward obtaining a particularly large library of Au nanocapsules encoded with Raman codes defined by the combination of different thiol-free Raman reporters, encapsulated at defined molar ratios. The fabrication of SERS tags with tailored size and predefined codes is based on the in situ incorporation of Raman reporter molecules inside Au nanocapsules during their formation via galvanic replacement coupled to seeded growth on Ag NPs. The hole-free closed-shell structure of the nanocapsules is confirmed by electron tomography. The unusually wide encoding possibilities of the obtained SERS tags are investigated by means of either wavenumber-based encoding or Raman frequency combined with signal intensity, leading to an outstanding performance as exemplified by 26 and 54 different codes, respectively. We additionally demonstrate that encoded nanocapsules can be readily bioconjugated with antibodies for applications such as SERS-based targeted cell imaging and phenotyping.", "gemini": "Các hạt nano vàng được mã hóa Raman (NP) đã được sử dụng rộng rãi như các đầu dò đa chức năng quang ổn định để cảm biến, chụp ảnh sinh học, chẩn đoán đa kênh và liệu pháp khối u được hướng dẫn bằng tán xạ Raman tăng cường bề mặt (SERS). Chúng tôi báo cáo một chiến lược hướng tới việc thu được một thư viện đặc biệt lớn các nanocapsule Au được mã hóa bằng các mã Raman được xác định bởi sự kết hợp của các chất báo cáo Raman không chứa thiol khác nhau, được đóng gói theo tỷ lệ mol đã xác định. Việc chế tạo các thẻ SERS có kích thước được thiết kế riêng và các mã được xác định trước dựa trên sự kết hợp tại chỗ các phân tử báo cáo Raman bên trong các nanocapsule Au trong quá trình hình thành của chúng thông qua sự thay thế điện hóa kết hợp với sự phát triển hạt giống trên các hạt Ag. Cấu trúc vỏ kín không lỗ của các nanocapsule được xác nhận bằng chụp cắt lớp điện tử. Các khả năng mã hóa rộng bất thường của các thẻ SERS thu được được nghiên cứu bằng cách mã hóa dựa trên số sóng hoặc tần số Raman kết hợp với cường độ tín hiệu, dẫn đến hiệu suất vượt trội được minh họa bằng 26 và 54 mã khác nhau. Ngoài ra, chúng tôi chứng minh rằng các nanocapsule được mã hóa có thể dễ dàng liên hợp sinh học với các kháng thể để ứng dụng như chụp ảnh tế bào mục tiêu dựa trên SERS và lập kiểu hình."} +{"idx": 1870347, "en": "Medical microrobots (MRs) have been demonstrated for a variety of non-invasive biomedical applications, such as tissue engineering, drug delivery, and assisted fertilization, among others. However, most of these demonstrations have been carried out in in vitro settings and under optical microscopy, being significantly different from the clinical practice. Thus, medical imaging techniques are required for localizing and tracking such tiny therapeutic machines when used in medical-relevant applications. This review aims at analyzing the state of the art of microrobots imaging by critically discussing the potentialities and limitations of the techniques employed in this field. Moreover, the physics and the working principle behind each analyzed imaging strategy, the spatiotemporal resolution, and the penetration depth are thoroughly discussed. The paper deals with the suitability of each imaging technique for tracking single or swarms of MRs and discusses the scenarios where contrast or imaging agent's inclusion is required, either to absorb, emit, or reflect a determined physical signal detected by an external system. Finally, the review highlights the existing challenges and perspective solutions which could be promising for future in vivo applications.", "gemini": "Các microrobot y tế (MR) đã được chứng minh cho nhiều ứng dụng y sinh không xâm lấn, chẳng hạn như kỹ thuật mô, cung cấp thuốc và hỗ trợ thụ tinh, trong số những ứng dụng khác. Tuy nhiên, hầu hết các cuộc trình diễn này đã được thực hiện trong môi trường trong ống nghiệm và dưới kính hiển vi quang học, khác biệt đáng kể so với thực hành lâm sàng. Do đó, các kỹ thuật hình ảnh y tế là cần thiết để định vị và theo dõi các máy điều trị nhỏ như vậy khi sử dụng trong các ứng dụng có liên quan đến y tế. Đánh giá này nhằm phân tích tình hình hiện tại của hình ảnh microrobot bằng cách thảo luận một cách phê phán về tiềm năng và hạn chế của các kỹ thuật được sử dụng trong lĩnh vực này. Hơn nữa, vật lý và nguyên lý hoạt động đằng sau mỗi chiến lược hình ảnh được phân tích, độ phân giải không gian thời gian và độ sâu thâm nhập được thảo luận kỹ lưỡng. Bài báo đề cập đến tính phù hợp của từng kỹ thuật hình ảnh để theo dõi từng MR hoặc đàn MR và thảo luận về các tình huống cần đưa chất tương phản hoặc tác nhân hình ảnh vào, để hấp thụ, phát ra hoặc phản xạ một tín hiệu vật lý xác định được phát hiện bởi hệ thống bên ngoài. Cuối cùng, đánh giá nêu bật những thách thức hiện tại và các giải pháp triển vọng có thể hứa hẹn cho các ứng dụng trong cơ thể trong tương lai."} +{"idx": 1870348, "en": "Cells are attractive carriers for the transport and delivery of nanoparticulate cargo. The use of cell-based carriers allows one to enhance control over the biodistribution of drug-loaded polymers and polymer nanoparticles. One key element in the development of cell-based delivery systems is the loading of the cell-based carrier with the nanoparticle cargo, which can be achieved either by internalization of the payload or by immobilization on the cell surface. The surface modification of cells with nanoparticles or the internalization of nanoparticles by cells is usually monitored with fluorescence-based techniques, such as flow cytometry and confocal microscopy. In spite of the widespread use of these techniques, the use of fluorescent labels also poses some risks and has several drawbacks. Fluorescent dyes may bleach, or leach from, the nanoparticles or alter the physicochemical properties of nanoparticles and their interactions with and uptake by cells. Using poly(d,l-lactic acid) nanoparticles that are loaded with Coumarin 6, BODIPY 493/503, or DiO dyes as a model system, this paper demonstrates that the use of physically entrapped fluorescent labels can lead to false negative or erroneous results. The use of nanoparticles that contain covalently tethered fluorescent dyes instead was found to provide a robust approach to monitor cell surface conjugation reactions and to quantitatively analyze nanoparticle-decorated cells. Finally, it is shown that optical diffraction tomography is an attractive, alternative technique for the characterization of nanoparticle-decorated cells, which obviates the need for fluorescent labels.", "gemini": "Các tế bào là chất mang hấp dẫn để vận chuyển và phân phối hàng hóa dạng hạt nano. Việc sử dụng chất mang gốc tế bào cho phép tăng cường kiểm soát sự phân bố sinh học của các polyme chứa thuốc và các hạt nano polyme. Một yếu tố chính trong sự phát triển của các hệ thống phân phối dựa trên tế bào là việc tải chất mang gốc tế bào với hàng hóa hạt nano, có thể đạt được bằng cách nội tại hóa tải trọng hoặc bằng cách cố định trên bề mặt tế bào. Việc sửa đổi bề mặt của các tế bào bằng các hạt nano hoặc quá trình nội tại hóa các hạt nano của các tế bào thường được theo dõi bằng các kỹ thuật dựa trên huỳnh quang, chẳng hạn như đo lưu lượng tế bào và kính hiển vi cộng hưởng. Mặc dù các kỹ thuật này được sử dụng rộng rãi, việc sử dụng nhãn huỳnh quang cũng gây ra một số rủi ro và có một số nhược điểm. Thuốc nhuộm huỳnh quang có thể bị tẩy trắng hoặc rò rỉ khỏi các hạt nano hoặc làm thay đổi các tính chất lý hóa của các hạt nano và sự tương tác của chúng với các tế bào cũng như quá trình hấp thụ của chúng. Sử dụng các hạt nano poly(d,l-axit lactic) được tải với Coumarin 6, BODIPY 493/503 hoặc thuốc nhuộm DiO làm hệ thống mô hình, bài báo này chứng minh rằng việc sử dụng nhãn huỳnh quang bị giữ lại vật lý có thể dẫn đến kết quả âm tính giả hoặc kết quả sai. Thay vào đó, việc sử dụng các hạt nano có chứa thuốc nhuộm huỳnh quang liên kết cộng hóa trị được phát hiện là cung cấp một phương pháp tiếp cận mạnh mẽ để theo dõi các phản ứng liên hợp bề mặt tế bào và để phân tích định lượng các tế bào được trang trí bằng hạt nano. Cuối cùng, người ta chỉ ra rằng chụp cắt lớp nhiễu xạ quang là một kỹ thuật thay thế hấp dẫn để đặc trưng các tế bào được trang trí bằng hạt nano, giúp loại bỏ nhu cầu sử dụng nhãn huỳnh quang."} +{"idx": 1870349, "en": "Bioactive nanomaterials have the potential to overcome the limitations of classical pharmacological approaches by taking advantage of native pathways to influence cell behavior, interacting with them and eliciting responses. Herein, we propose a cascade system mediated by two catalytic nanocompartments (CNC) with biological activity. Activated by nitric oxide (NO) produced by inducible nitric oxidase synthase (iNOS), soluble guanylyl cyclase (sGC) produces cyclic guanosine monophosphate (cGMP), a second messenger that modulates a broad range of physiological functions. As alterations in cGMP signaling are implicated in a multitude of pathologies, its signaling cascade represents a viable target for therapeutic intervention. Following along this line, we encapsulated iNOS and sGC in two separate polymeric compartments that function in unison to produce NO and cGMP. Their action was tested in vitro by monitoring the derived changes in cytoplasmic calcium concentrations of HeLa and differentiated C2C12 myocytes, where the produced second messenger influenced the cellular homeostasis.", "gemini": "Các vật liệu nano hoạt tính sinh học có tiềm năng khắc phục những hạn chế của các phương pháp dược lý cổ điển bằng cách tận dụng các con đường bản địa để ảnh hưởng đến hành vi của tế bào, tương tác với chúng và gây ra phản ứng. Ở đây, chúng tôi đề xuất một hệ thống thác được trung gian bởi hai khoang nano xúc tác (CNC) có hoạt tính sinh học. Được kích hoạt bởi nitric oxide (NO) do synthase nitric oxide có thể gây ra (iNOS), guanylyl cyclase hòa tan (sGC) tạo ra cyclic guanosine monophosphate (cGMP), một chất truyền tin thứ hai điều chỉnh nhiều chức năng sinh lý. Vì những thay đổi trong tín hiệu cGMP liên quan đến vô số bệnh lý, nên chuỗi tín hiệu của nó đại diện cho một mục tiêu khả thi để can thiệp điều trị. Theo hướng này, chúng tôi đã đóng gói iNOS và sGC trong hai khoang polyme riêng biệt có chức năng hợp nhất để sản xuất NO và cGMP. Hoạt động của chúng đã được thử nghiệm trong ống nghiệm bằng cách theo dõi những thay đổi có nguồn gốc trong nồng độ canxi trong tế bào chất của HeLa và tế bào cơ C2C12 biệt hóa, nơi chất truyền tin thứ hai được sản xuất ảnh hưởng đến cân bằng nội môi của tế bào."} +{"idx": 1870350, "en": "Capacitive deionization (CDI) is a promising water desalination technology that is applicable to the treatment of low-salinity brackish waters and the selective removal of ionic contaminants. In this work, we show that by making a small change in the synthetic procedure of hierarchical carbon aerogel monolith (HCAM) electrodes, we can adjust the pore-size distribution and tailor the selectivity, effectively switching between selective adsorption of calcium or sodium ions. Ion selectivity was measured for a mixture of 5 mM NaCl and 2.5 mM CaCl2. For the low activated flow-through CDI (fteCDI) cell, we observed extremely high sodium selectivity over calcium (SNa/Ca ≫ 10, no Ca2+ adsorbed) at all of the applied potentials, while for the highly activated fteCDI cell, we observed a selectivity value of 6.6 ± 0.8 at 0.6 V for calcium over sodium that decreased to 2.2 ± 0.03 at 1.2 V. Molecular dynamics simulations indicated that the loss in Ca2+ selectivity over Na+ at high applied voltages could be due to a competition between ion-pore electrostatic interactions and volume exclusion (\"crowding\") effects. Interestingly, we also find with these simulations that the relative sizes of the ions change due to changes in hydration at a higher voltage.", "gemini": "Ion hóa điện dung (CDI) là một công nghệ khử muối nước đầy hứa hẹn có thể áp dụng để xử lý nước lợ có độ mặn thấp và loại bỏ chọn lọc các chất gây ô nhiễm ion. Trong công trình này, chúng tôi chỉ ra rằng bằng cách thực hiện một thay đổi nhỏ trong quy trình tổng hợp các điện cực khối khí carbon phân cấp (HCAM), chúng tôi có thể điều chỉnh phân bố kích thước lỗ và điều chỉnh tính chọn lọc, chuyển đổi hiệu quả giữa sự hấp phụ chọn lọc các ion canxi hoặc natri. Tính chọn lọc ion được đo cho hỗn hợp 5 mM NaCl và 2,5 mM CaCl2. Đối với tế bào CDI dòng chảy thấp được kích hoạt (fteCDI), chúng tôi quan sát thấy tính chọn lọc natri cực cao so với canxi (SNa/Ca ≫ 10, không hấp phụ Ca2+) ở tất cả các điện thế được áp dụng, trong khi đối với tế bào fteCDI được kích hoạt cao, chúng tôi quan sát thấy giá trị chọn lọc là 6,6 ± 0,8 ở 0,6 V đối với canxi so với natri giảm xuống 2,2 ± 0,03 ở 1,2 V. Các mô phỏng động học phân tử chỉ ra rằng sự mất tính chọn lọc Ca2+ so với Na+ ở điện áp cao có thể là do sự cạnh tranh giữa các tương tác tĩnh điện ion-lỗ và các hiệu ứng loại trừ thể tích (\"chen lấn\"). Điều thú vị là chúng tôi cũng thấy với các mô phỏng này rằng kích thước tương đối của các ion thay đổi do sự thay đổi về hydrat hóa ở điện áp cao hơn."} +{"idx": 1870351, "en": "Exploitation of high-efficiency and low-cost catalysts for dehydrogenation of the ideal hydrogen storage material (ammonia borane) can effectively promote the development of hydrogen economy. Here, we report an efficient and economical non-noble-metal magnetic catalyst (Ni0.23Co0.19P0.58@NHPC900) with nanoparticles uniformly distributed on MOF-derived (metal-organic framework) nitrogen-doped hierarchical porous carbon (NHPC900) by a one-step in situ synthesis method. The catalyst has achieved a superior initial total turnover frequency (TOF) of 125.2 molH2·molcat-1·min-1. Based on isotopic analyses and ion effects, we further obtain an unprecedentedly higher TOF of 282.4 molH2·molcat-1·min-1, the highest among non-noble-metal heterogeneous systems. Through experiments and theoretical studies, we confirm that the highly doped phosphorus component leads to a C-P-Ni-Co quaternary synergy in the catalyst. Then, the induced strong electron transfer and increased partial charge can reduce the reaction energy barrier, strengthen the adsorption of ammonia borane, and ultimately result in superior catalytic performance. The proposed mechanisms and strategies are helpful to develop non-noble-metal catalysts for practical applications of hydrogen energy systems in the future.", "gemini": "Việc khai thác các chất xúc tác hiệu suất cao và chi phí thấp để khử hydro vật liệu lưu trữ hydro lý tưởng (amoniac boran) có thể thúc đẩy hiệu quả sự phát triển của nền kinh tế hydro. Tại đây, chúng tôi báo cáo một chất xúc tác từ tính phi kim loại quý hiệu quả và tiết kiệm (Ni0,23Co0,19P0,58@NHPC900) với các hạt nano phân bố đều trên cacbon xốp phân cấp được nitơ hóa (NHPC900) có nguồn gốc từ MOF (khung hữu cơ kim loại) bằng phương pháp tổng hợp tại chỗ một bước. Chất xúc tác đã đạt được tần số tuần hoàn tổng ban đầu (TOF) vượt trội là 125,2 molH2·molcat-1·phút-1. Dựa trên các phân tích đồng vị và hiệu ứng ion, chúng tôi tiếp tục thu được TOF cao hơn chưa từng có là 282,4 molH2·molcat-1·phút-1, cao nhất trong số các hệ thống không phải kim loại quý không đồng nhất. Thông qua các thí nghiệm và nghiên cứu lý thuyết, chúng tôi xác nhận rằng thành phần phốt pho được pha tạp cao dẫn đến sự hiệp đồng bậc bốn C-P-Ni-Co trong chất xúc tác. Sau đó, quá trình truyền electron mạnh được tạo ra và tăng điện tích riêng phần có thể làm giảm rào cản năng lượng phản ứng, tăng cường sự hấp phụ của amoniac boran và cuối cùng dẫn đến hiệu suất xúc tác vượt trội. Các cơ chế và chiến lược được đề xuất rất hữu ích để phát triển các chất xúc tác phi kim loại quý cho các ứng dụng thực tế của hệ thống năng lượng hydro trong tương lai."} +{"idx": 1870352, "en": "Apolipoprotein E (apoE) polymorphic genes are one of the main genetic determinants of Alzheimer's disease (AD) risk. Relying on the toehold-mediated strand displacement reaction (SDR), the dual-signal electrochemical assay of apoE genotyping with potential applications in the early diagnosis of AD has been achieved. The displacement of the surface-confined methylene blue- and ferrocene-capped detection probe-modified gold nanoparticles (AuNPs) by the complementary sequences (Tc 1 and Tc 2, fragment of allele ε4 at codon 112 and that of allele ε3 or ε4 at codon 158, respectively), triggered by the highly specific SDR, results in decreased voltammetric signals. In contrast, partial strand displacement caused by the single mismatched sequences (Tsm 1 and Tsm 2, fragment of allele ε2 or ε3 at codon 112 and that of allele ε2 at codon 158, respectively) produces larger voltammetric signals. The proposed method serves as a versatile platform for the discrimination of six apoE genotypes, including three homozygotes (ε2/2, ε3/3, and ε4/4) and three heterozygotes (ε2/3, ε2/4, and ε3/4), and for the quantification of apoE ε3/3 from genomic DNA extracts of AD patients.", "gemini": "Các gen đa hình của apolipoprotein E (apoE) là một trong những yếu tố di truyền chính quyết định nguy cơ mắc bệnh Alzheimer (AD). Dựa vào phản ứng dịch chuyển sợi do chỗ bám khởi đầu (SDR), xét nghiệm điện hóa tín hiệu kép về kiểu gen apoE có các ứng dụng tiềm năng trong chẩn đoán sớm bệnh AD đã đạt được. Sự dịch chuyển của các hạt nano vàng (AuNP) được sửa đổi bằng đầu dò phát hiện có nắp ferrocene và xanh methylen giới hạn trên bề mặt bởi các trình tự bổ sung (Tc 1 và Tc 2, đoạn của alen ε4 ở codon 112 và đoạn của alen ε3 hoặc ε4 ở codon 158, tương ứng), được kích hoạt bởi SDR có tính đặc hiệu cao, dẫn đến giảm tín hiệu voltam. Ngược lại, sự dịch chuyển sợi một phần do các trình tự không khớp đơn (Tsm 1 và Tsm 2, đoạn của alen ε2 hoặc ε3 ở codon 112 và đoạn của alen ε2 ở codon 158, tương ứng) tạo ra các tín hiệu voltam lớn hơn. Phương pháp được đề xuất đóng vai trò là một nền tảng linh hoạt để phân biệt sáu kiểu gen apoE, bao gồm ba kiểu đồng hợp tử (ε2/2, ε3/3 và ε4/4) và ba kiểu dị hợp tử (ε2/3, ε2/4 và ε3/4), và để định lượng apoE ε3/3 từ các đoạn trích DNA của bệnh nhân AD."} +{"idx": 1870353, "en": "There has been a relentless pursuit of transverse electric (TE)-dominant deep ultraviolet (UV) optoelectronic devices for efficient surface emitters to replace the environmentally unfriendly mercury lamps. To date, the use of the ternary AlGaN alloy inevitably has led to transverse magnetic (TM)-dominant emission, an approach that is facing a roadblock. Here, we take an entirely different approach of utilizing a binary GaN compound semiconductor in conjunction with ultrathin quantum disks (QDisks) embedded in AlN nanowires (NWs). The growth of GaN QDisks is realized on a scalable and low-cost Si substrate using plasma-assisted molecular beam epitaxy as a highly controllable monolayer growth platform. We estimated an internal quantum efficiency of ∼81% in a wavelength regime of ∼260 nm for these nanostructures. Additionally, strain mapping obtained by high-angle annular dark-field scanning transmission electron microscopy is studied in conjunction with the TE and TM modes of the carrier recombination. Moreover, for the first time, we quantify the TE and TM modes of the PL emitted by GaN QDisks for deep-UV emitters. We observed nearly pure TE-polarized photoluminescence emission at a polarization angle of ∼5°. This work proposes highly quantum-confined ultrathin GaN QDisks as a promising candidate for deep-UV vertical emitters.", "gemini": "Đã có một cuộc theo đuổi không ngừng đối với các thiết bị quang điện tử tử ngoại sâu (UV) chủ yếu là điện trường ngang (TE) để thay thế các đèn thủy ngân không thân thiện với môi trường. Cho đến nay, việc sử dụng hợp kim AlGaN ba thành phần chắc chắn đã dẫn đến phát xạ chủ yếu là từ trường ngang (TM), một phương pháp đang phải đối mặt với rào cản. Tại đây, chúng tôi thực hiện một cách tiếp cận hoàn toàn khác là sử dụng chất bán dẫn hợp chất GaN nhị phân kết hợp với các đĩa lượng tử siêu mỏng (QDisk) được nhúng trong các nanowire AlN (NW). Sự phát triển của GaN QDisk được thực hiện trên một chất nền Si có thể mở rộng và chi phí thấp bằng cách sử dụng phương pháp epitaxy chùm phân tử hỗ trợ plasma làm nền tảng phát triển đơn lớp có thể kiểm soát cao. Chúng tôi ước tính hiệu suất lượng tử bên trong là ∼81% trong chế độ bước sóng ∼260 nm cho các cấu trúc nano này. Ngoài ra, ánh xạ ứng suất thu được bằng kính hiển vi điện tử truyền qua trường tối góc cao được nghiên cứu kết hợp với các chế độ TE và TM của sự tái hợp của hạt tải. Hơn nữa, lần đầu tiên, chúng tôi định lượng các chế độ TE và TM của PL phát ra bởi GaN QDisk đối với các bộ phát UV sâu. Chúng tôi quan sát thấy phát xạ quang phát quang gần như phân cực TE ở góc phân cực ∼5°. Công trình này đề xuất các GaN QDisk siêu mỏng hạn chế lượng tử cao là ứng cử viên đầy hứa hẹn cho các bộ phát dọc UV sâu."} +{"idx": 1870354, "en": "Plastics pose ecological and human health risks, with disposable contact lenses constituting a potential high-volume pollution source. Using sales data and an online survey of lens users (n = 416) alongside laboratory and field experiments at a conventional sewage treatment plant, we determined the environmental fate and mass inventories of contact lenses in the United States. The survey results revealed that 21 ± 0.8% of lens users flush their used lenses down the drain, a loading equivalent to 44 000 ± 1700 kg y-1 of lens dry mass discharged into US wastewater. Biological treatment of wastewater did not result in a measurable loss of plastic mass (p = 0.001) and caused only very limited changes in the polymer structure, as determined by μ-Raman spectroscopy. During sewage treatment, the lenses were found to accumulate as fragments in sewage sludge, resulting in an estimated accumulation of 24 000 ± 940 kg y-1 of microplastics destined for application on US agricultural soils contained in sewage sludge. Recycling of the contact lenses and their packaging amounted to only 0.04% of the total waste volume associated with contact lens use. This is the first study to identify contact lenses and more specifically silicone hydrogels, as a previously overlooked source of plastic and microplastic pollution.", "gemini": "Nhựa gây ra rủi ro cho sức khỏe con người và sinh thái, trong đó kính áp tròng dùng một lần là nguồn gây ô nhiễm tiềm ẩn với khối lượng lớn. Sử dụng dữ liệu bán hàng và khảo sát trực tuyến những người dùng kính áp tròng (n = 416) cùng với các thí nghiệm tại phòng thí nghiệm và thực địa tại một nhà máy xử lý nước thải thông thường, chúng tôi đã xác định được số phận của môi trường và lượng tồn kho kính áp tròng tại Hoa Kỳ. Kết quả khảo sát cho thấy 21 ± 0,8% người dùng kính áp tròng xả kính đã qua sử dụng xuống cống, tương đương với lượng tải 44.000 ± 1700 kg y-1 khối lượng khô của kính thải vào nước thải của Hoa Kỳ. Việc xử lý nước thải bằng phương pháp sinh học không làm mất khối lượng nhựa có thể đo lường được (p = 0,001) và chỉ gây ra những thay đổi rất nhỏ trong cấu trúc polyme, như được xác định bằng quang phổ μ-Raman. Trong quá trình xử lý nước thải, người ta thấy rằng các thấu kính tích tụ thành các mảnh trong bùn thải, dẫn đến ước tính tích tụ 24.000 ± 940 kg y-1 vi nhựa được chứa trong bùn thải dùng để bón cho đất nông nghiệp ở Hoa Kỳ. Việc tái chế kính áp tròng và bao bì của chúng chỉ chiếm 0,04% tổng lượng chất thải liên quan đến việc sử dụng kính áp tròng. Đây là nghiên cứu đầu tiên xác định kính áp tròng và cụ thể hơn là hydrogel silicon, là một nguồn ô nhiễm nhựa và vi nhựa trước đây bị bỏ qua."} +{"idx": 1870355, "en": "The synthesis and photophysical study of two dinuclear copper(I) complexes bearing a 2-(1H-imidazol-2-yl)pyrimidine bridging ligand are described. The tetrahedral coordination sphere of each copper center is completed through the use of a bulky bis(phosphine) ligand, either DPEphos or Xantphos. Temperature-dependent photophysical studies demonstrated emission through a combination of phosphorescence and thermally activated delayed fluorescence for both complexes, and an intense emission (ΦPL = 46%) was observed for a crystalline sample of one of the complexes reported. The photophysics of these two complexes is very sensitive to the environment. Two pseudopolymorphs of one of the dinuclear complexes were isolated, with distinct photophysics. The emission color of the crystals can be changed by grinding, and the differences in their photophysics before and after grinding are discussed.", "gemini": "Tổng hợp và nghiên cứu quang lý của hai phức hợp đồng(I) nhân đôi có phối tử cầu nối 2-(1H-imidazol-2-yl)pyrimidine được mô tả. Mỗi trung tâm đồng có cấu trúc tứ diện được hoàn thiện thông qua việc sử dụng phối tử bis(phosphine) cồng kềnh, có thể là DPEphos hoặc Xantphos. Các nghiên cứu quang lý phụ thuộc nhiệt độ chứng minh sự phát xạ thông qua sự kết hợp của sự phát quang và huỳnh quang trễ kích hoạt nhiệt cho cả hai phức hợp và sự phát xạ mạnh (ΦPL = 46%) được quan sát thấy đối với mẫu tinh thể của một trong các phức hợp được báo cáo. Quang lý của hai phức hợp này rất nhạy cảm với môi trường. Hai dạng giả đa hình của một trong các phức hợp nhân đôi đã được phân lập, với quang lý riêng biệt. Màu phát xạ của các tinh thể có thể thay đổi bằng cách nghiền, và sự khác biệt về quang lý của chúng trước và sau khi nghiền được thảo luận."} +{"idx": 1870356, "en": "The electronic excited states of the iron(II) complex [FeII(tpy)(pyz-NHC)]2+ [tpy = 2,2':6',2″-terpyridine; pyz-NHC = 1,1'-bis(2,6-diisopropylphenyl)pyrazinyldiimidazolium-2,2'-diylidene] and their relaxation pathways have been theoretically investigated. To this purpose, trajectory surface-hopping simulations within a linear vibronic coupling model including a 244-dimensional potential energy surface (PES) with 20 singlet and 20 triplet coupled states have been used. The simulations show that, after excitation to the lowest-energy absorption band of predominant metal-to-ligand charge-transfer character involving the tpy ligand, almost 80% of the population undergoes intersystem crossing to the triplet manifold in about 50 fs, while the remaining 20% decays through internal conversion to the electronic ground state in about 300 fs. The population transferred to the triplet states is found to deactivate into two different regions of the PESs, one where the static dipole moment is small and shows increased metal-centered character and another with a large static dipole moment, where the electron density is transferred from the tpy to pyz-NHC ligand. Coherent oscillations of 400 fs are observed between these two sets of triplet populations, until the mixture equilibrates to a ratio of 60:40. Finally, the importance of selecting suitable normal modes is highlighted-a choice that can be far from straightforward in transition-metal complexes with hundreds of degrees of freedom.", "gemini": "Các trạng thái kích thích điện tử của phức hợp sắt(II) [FeII(tpy)(pyz-NHC)]2+ [tpy = 2,2':6',2″-terpyridine; pyz-NHC = 1,1'-bis(2,6-diisopropylphenyl)pyrazinyldiimidazolium-2,2'-diylidene] và các đường dẫn giãn của chúng đã được nghiên cứu về mặt lý thuyết. Với mục đích này, các mô phỏng nhảy bề mặt quỹ đạo trong một mô hình liên hợp rung động tuyến tính bao gồm bề mặt năng lượng thế năng 244 chiều (PES) với 20 trạng thái đơn và 20 trạng thái ba được ghép nối đã được sử dụng. Các mô phỏng cho thấy rằng, sau khi kích thích đến dải hấp thụ năng lượng thấp nhất có đặc tính chuyển điện tích kim loại-ligand chiếm ưu thế liên quan đến phối tử tpy, gần 80% dân số trải qua sự giao thoa hệ thống liên quan đến đa tạp ba trong khoảng 50 fs, trong khi 20% còn lại phân rã thông qua chuyển đổi bên trong đến trạng thái cơ bản điện tử trong khoảng 300 fs. Dân số được chuyển đến các trạng thái ba được phát hiện là bất hoạt thành hai vùng khác nhau của PES, một vùng có mômen lưỡng cực tĩnh nhỏ và cho thấy đặc tính tập trung vào kim loại tăng lên và một vùng khác có mômen lưỡng cực tĩnh lớn, nơi mật độ electron được chuyển từ tpy sang phối tử pyz-NHC. Dao động mạch lạc 400 fs được quan sát thấy giữa hai tập hợp dân số ba này, cho đến khi hỗn hợp cân bằng theo tỷ lệ 60:40. Cuối cùng, tầm quan trọng của việc lựa chọn các chế độ bình thường phù hợp được nêu bật - một lựa chọn có thể không đơn giản đối với các phức hợp kim loại chuyển tiếp với hàng trăm bậc tự do."} +{"idx": 1870357, "en": "Recent advancements in immunocapture methods and mass spectrometer technology have enabled intact protein mass spectrometry to be applied for the characterization of antibodies and other large biotherapeutics from in-life studies. Protein molecules have not been traditionally studied by intact mass or screened for catabolites in the same manner as small molecules, but the landscape has changed. Researchers have presented methods that can be applied to the drug discovery and development stages, and others are exploring the possibilities of the new approaches. However, a wide variety of options for assay development exists without clear recommendation on best practice, and data processing workflows may have limitations depending on the vendor. In this perspective, we share experiences and recommendations for current and future application of mass spectrometry for biotherapeutic molecule monitoring from preclinical and clinical studies.", "gemini": "Những tiến bộ gần đây trong các phương pháp bắt giữ miễn dịch và công nghệ phổ khối đã cho phép phổ khối protein nguyên vẹn được áp dụng để đặc trưng hóa các kháng thể và các chất sinh học lớn khác từ các nghiên cứu trong đời sống. Các phân tử protein theo truyền thống không được nghiên cứu bằng khối lượng nguyên vẹn hoặc được sàng lọc chất chuyển hóa theo cùng cách như các phân tử nhỏ, nhưng bối cảnh đã thay đổi. Các nhà nghiên cứu đã trình bày các phương pháp có thể được áp dụng cho các giai đoạn phát hiện và phát triển thuốc, và những phương pháp khác đang khám phá khả năng của các phương pháp tiếp cận mới. Tuy nhiên, có rất nhiều lựa chọn để phát triển xét nghiệm mà không có khuyến nghị rõ ràng về phương pháp thực hành tốt nhất và quy trình xử lý dữ liệu có thể có những hạn chế tùy thuộc vào nhà cung cấp. Trong góc nhìn này, chúng tôi chia sẻ kinh nghiệm và khuyến nghị cho ứng dụng phổ khối hiện tại và tương lai để theo dõi phân tử sinh học từ các nghiên cứu tiền lâm sàng và lâm sàng."} +{"idx": 1870358, "en": "Engineered nano-bio interfaces driven by tunable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Yet the interplay between substrate topography and cellular behavior is highly complex and not fully understood. A new experimental design is proposed that enables generation of ultrathin sections (lamellae) of cell-nanostructure imprints with minimal artifacts. We demonstrate the potential of such lamellae for efficient transmission electron microscopy (TEM) characterization of interfacial interactions between adherent cells and vertically aligned Si nanostructures. This approach will advance understanding of cellular responses to extracellular biophysical and biochemical cues-which is likely to facilitate the design of improved cellular manipulation technologies.", "gemini": "Các giao diện nano-sinh học được thiết kế theo các cấu trúc nano có thể cấu hình theo chiều dọc gần đây đã nổi lên như một công cụ mạnh mẽ để thao tác và thẩm vấn tế bào. Tuy nhiên, sự tương tác giữa địa hình nền và hành vi của tế bào rất phức tạp và chưa được hiểu đầy đủ. Một thiết kế thử nghiệm mới được đề xuất cho phép tạo ra các phần siêu mỏng (lamellae) của dấu ấn cấu trúc nano tế bào với các hiện vật tối thiểu. Chúng tôi chứng minh tiềm năng của các lamellae như vậy để đặc tính hóa tương tác giao diện giữa các tế bào bám dính và các cấu trúc nano Si xếp thẳng đứng bằng kính hiển vi điện tử truyền qua (TEM) hiệu quả. Phương pháp tiếp cận này sẽ nâng cao sự hiểu biết về phản ứng của tế bào đối với các tín hiệu sinh học và hóa học ngoại bào, có khả năng tạo điều kiện thuận lợi cho việc thiết kế các công nghệ thao tác tế bào được cải tiến."} +{"idx": 1870359, "en": "We have synthesized and structurally characterized the 4-palladium(II)-containing 30-tungsto-4-arsenate(V), [Pd4(As2W15O56)2]16- (1), which represents the first palladium(II)-containing tungstoarsenate(V). The title polyanion 1 was prepared by a simple one-pot procedure in aqueous medium and characterized by single-crystal X-ray diffraction (XRD), thermogravimetric analysis (TGA), cyclic voltammetry, elemental analysis, and 183W nuclear magnetic resonance (NMR), infrared (IR), and ultraviolet-visible (UV-vis) spectroscopies. Polyanion 1 consists of four Pd2+ ions that are coordinated in a square-planar geometry to two trilacunary [As2W15O56]12- Wells-Dawson fragments resulting in a sandwich-type assembly. Catalytic studies on 1 revealed that it is an efficient catalyst precursor for the Suzuki-Miyaura cross-coupling reactions of various aryl halides in aqueous and nonaqueous media.", "gemini": "Chúng tôi đã tổng hợp và đặc trưng về mặt cấu trúc 4-palladium (II) chứa 30-tungsto-4-arsenat (V), [Pd4 (As2W15O56) 2] 16- (1), đại diện cho tungstoarsenat (V) chứa palladium (II) đầu tiên. Polyanion 1 có tiêu đề đã được điều chế bằng quy trình một nồi đơn giản trong môi trường nước và được đặc trưng bằng nhiễu xạ tia X tinh thể đơn (XRD), phân tích nhiệt trọng lượng (TGA), voltampe kế tuần hoàn, phân tích nguyên tố và cộng hưởng từ hạt nhân 183W (NMR), hồng ngoại (IR) và tử ngoại-quang phổ khả kiến (UV-vis). Polyanion 1 bao gồm bốn ion Pd2 + được phối hợp trong hình học mặt phẳng hình vuông với hai mảnh Wells-Dawson [As2W15O56] 12- ba lỗ trống dẫn đến lắp ráp kiểu sandwich. Các nghiên cứu xúc tác trên 1 cho thấy đây là tiền chất xúc tác hiệu quả cho phản ứng ghép nối chéo Suzuki-Miyaura của nhiều loại aryl halide trong môi trường nước và không chứa nước."} +{"idx": 1870360, "en": "The success of organic-inorganic perovskites in optoelectronics is dictated by the complex interplay between various underlying microscopic phenomena. The structural dynamics of organic cations and the inorganic sublattice after photoexcitation are hypothesized to have a direct effect on the material properties, thereby affecting the overall device performance. Here, we use ultrafast heterodyne-detected two-dimensional (2D) electronic spectroscopy to reveal impulsively excited vibrational modes of methylammonium (MA) lead iodide perovskite, which drive the structural distortion after photoexcitation. Vibrational analysis of the measured data allows us to monitor the time-evolved librational motion of the MA cation along with the vibrational coherences of the inorganic sublattice. Wavelet analysis of the observed vibrational coherences reveals the coherent generation of the librational motion of the MA cation within ∼300 fs complemented with the coherent evolution of the inorganic skeletal motion. To rationalize this observation, we employed the configuration interaction singles (CIS), which support our experimental observations of the coherent generation of librational motions in the MA cation and highlight the importance of the anharmonic interaction between the MA cation and the inorganic sublattice. Moreover, our advanced theoretical calculations predict the transfer of the photoinduced vibrational coherence from the MA cation to the inorganic sublattice, leading to reorganization of the lattice to form a polaronic state with a long lifetime. Our study uncovers the interplay of the organic cation and inorganic sublattice during formation of the polaron, which may lead to novel design principles for the next generation of perovskite solar cell materials.", "gemini": "Sự thành công của perovskite hữu cơ-vô cơ trong quang điện tử được quyết định bởi sự tương tác phức tạp giữa nhiều hiện tượng vi mô cơ bản khác nhau. Giả thuyết cho rằng động lực học cấu trúc của các cation hữu cơ và mạng phụ vô cơ sau khi kích thích ánh sáng có tác động trực tiếp đến các tính chất của vật liệu, do đó ảnh hưởng đến hiệu suất tổng thể của thiết bị. Ở đây, chúng tôi sử dụng phổ học điện tử hai chiều (2D) phát hiện dị tần siêu nhanh để tiết lộ các chế độ rung động được kích thích đột ngột của metylamoni (MA) chì iốt perovskite, chúng thúc đẩy sự biến dạng cấu trúc sau khi kích thích ánh sáng. Phân tích rung động của dữ liệu đo được cho phép chúng tôi theo dõi chuyển động dao động theo thời gian của cation MA cùng với sự kết hợp rung động của mạng phụ vô cơ. Phân tích sóng con của các sự kết hợp rung động quan sát được cho thấy sự tạo ra đồng thời chuyển động dao động của cation MA trong vòng ∼300 fs bổ sung với sự tiến hóa đồng thời của chuyển động bộ xương vô cơ. Để hợp lý hóa quan sát này, chúng tôi sử dụng các cấu hình tương tác đơn (CIS), hỗ trợ các quan sát thực nghiệm của chúng tôi về sự tạo ra đồng thời các chuyển động dao động trong cation MA và làm nổi bật tầm quan trọng của sự tương tác phi hài hòa giữa cation MA và mạng phụ vô cơ. Hơn nữa, các tính toán lý thuyết nâng cao của chúng tôi dự đoán sự truyền sự kết hợp rung động quang cảm ứng từ cation MA sang mạng phụ vô cơ, dẫn đến sự sắp xếp lại của mạng để tạo thành trạng thái polaron có thời gian tồn tại lâu. Nghiên cứu của chúng tôi khám phá sự tương tác của cation hữu cơ và mạng phụ vô cơ trong quá trình hình thành polaron, điều này có thể dẫn đến các nguyên tắc thiết kế mới cho thế hệ vật liệu pin mặt trời perovskite tiếp theo."} +{"idx": 1870361, "en": "Understanding magnetic anisotropy and specifically how to tailor it is crucial in the search for high-temperature single-ion magnets. Herein, we investigate the magnetic anisotropy in a six-coordinated cobalt(II) compound that has a complex geometry and distinct triaxial magnetic anisotropy from the perspective of the electronic structure, using electronic spectra, ab initio calculations, and an experimental charge density, of which the latter two provides insight into the d-orbital splitting. The analysis showed that the d-orbital splitting satisfactorily predicted the complex triaxial magnetic anisotropy exhibited by the compound. Furthermore, a novel method to directly compare the ab initio results and the d-orbital populations obtained from the experimental charge density was developed, while a topological analysis of the density provided insights into the metal-ligand bonding. This work thus further establishes the validity of using d-orbitals for predicting magnetic anisotropy in transition metal compounds while also pointing out the need for a more frequent usage of the term triaxial anisotropy in the field of single-molecule magnetism.", "gemini": "Hiểu được tính dị hướng từ và cụ thể là cách điều chỉnh tính chất này là rất quan trọng trong việc tìm kiếm các nam châm ion đơn có nhiệt độ cao. Trong bài báo này, chúng tôi nghiên cứu tính dị hướng từ trong một hợp chất cobalt(II) được phối hợp sáu có hình học phức tạp và tính dị hướng từ ba trục riêng biệt theo góc nhìn của cấu trúc điện tử, sử dụng phổ điện tử, các tính toán ab initio và mật độ điện tích thực nghiệm, trong đó hai phương pháp sau cung cấp thông tin chi tiết về sự tách d-orbital. Phân tích cho thấy sự tách d-orbital dự đoán một cách thỏa đáng tính dị hướng từ ba trục phức tạp mà hợp chất thể hiện. Hơn nữa, một phương pháp mới để so sánh trực tiếp các kết quả ab initio và các quần thể d-orbital thu được từ mật độ điện tích thực nghiệm đã được phát triển, trong khi phân tích tôpô của mật độ cung cấp thông tin chi tiết về liên kết kim loại-ligand. Do đó, công trình này tiếp tục khẳng định tính hợp lệ của việc sử dụng d-orbital để dự đoán tính dị hướng từ trong các hợp chất kim loại chuyển tiếp đồng thời cũng chỉ ra nhu cầu sử dụng thường xuyên hơn thuật ngữ dị hướng ba trục trong lĩnh vực từ tính phân tử đơn."} +{"idx": 1870362, "en": "Two complementary regiodivergent [(P,N)Ni]-catalyzed hydroalkylations of branched dienes are reported. When amides are employed as unstabilized C(sp3) nucleophiles, a highly regioselective 1,4-addition process is favored. The addition products are obtained in high yield and with excellent stereocontrol of the internal olefin. With use of a chiral ligand and imides as carbon nucleophiles, a 3,4-addition protocol was developed, enabling construction of two contiguous tertiary stereocenters in a single step with moderate to high levels of diastereocontrol and excellent enantiocontrol. Both methods operate under mild reaction conditions, display a broad scope, and show excellent functional group tolerance. The synthetic potential of the 3,4-hydroalkylation reaction was established via a series of postcatalytic modifications.", "gemini": "Hai phản ứng hydroalkyl hóa [(P,N)Ni] theo hướng bổ sung nhau của đien phân nhánh được báo cáo. Khi sử dụng amit làm chất ái nhân C(sp3) không ổn định, quá trình cộng 1,4 có tính chọn lọc cao theo vùng được ưu tiên. Các sản phẩm cộng thu được với hiệu suất cao và khả năng kiểm soát lập thể tuyệt vời của olefin bên trong. Với việc sử dụng phối tử kiral và imid làm chất ái nhân cacbon, một giao thức cộng 3,4 đã được phát triển, cho phép xây dựng hai tâm lập thể bậc ba liền kề trong một bước với mức độ kiểm soát lập thể không gian vừa phải đến cao và kiểm soát quang học tuyệt vời. Cả hai phương pháp đều hoạt động trong điều kiện phản ứng nhẹ, thể hiện phạm vi rộng và dung nạp nhóm chức năng tuyệt vời. Tiềm năng tổng hợp của phản ứng hydroalkyl hóa 3,4 đã được thiết lập thông qua một loạt các sửa đổi sau xúc tác."} +{"idx": 1870363, "en": "Filtering of nonspecifically binding contaminant proteins from affinity purification mass spectrometry (AP-MS) data is a well-established strategy to improve statistical confidence in identified proteins. The CRAPome (contaminant repository for affinity purification) describes the contaminating background content present in many purification strategies. However, full contaminant lists for nickel-nitrilotriacetic acid (NiNTA) and glutathione S-transferase (GST) affinity matrices are lacking. Similarly, no Spodoptera frugiperda (Sf9) contaminants are available, and only the FLAG-purified contaminants are described for Escherichia coli. For MS experiments that use recombinant protein, such as structural mass spectrometry experiments (hydrogen-deuterium exchange mass spectrometry (HDX-MS), chemical cross-linking, and radical foot-printing), failing to include these contaminants in the search database during the initial tandem MS (MS/MS) identification stage can result in complications in peptide identification. We have created contaminant FASTA databases for Sf9 and E. coli NiNTA or GST purification strategies and show that the use of these databases can effectively improve HDX-MS protein coverage, fragment count, and confidence in peptide identification. This approach provides a robust strategy toward the design of contaminant databases for any purification approach that will expand the complexity of systems able to be interrogated by HDX-MS.", "gemini": "Việc lọc các protein tạp chất liên kết không đặc hiệu từ dữ liệu phổ khối sắc ký tinh chế ái lực (AP-MS) là một chiến lược được thiết lập tốt để cải thiện độ tin cậy thống kê đối với các protein đã xác định. CRAPome (kho tạp chất để tinh chế ái lực) mô tả nội dung nền tạp chất có trong nhiều chiến lược tinh chế. Tuy nhiên, danh sách tạp chất đầy đủ cho các ma trận ái lực niken-nitrilotriacetic (NiNTA) và glutathione S-transferase (GST) vẫn còn thiếu. Tương tự như vậy, không có tạp chất Spodoptera frugiperda (Sf9) nào có sẵn và chỉ có các tạp chất được làm sạch bằng FLAG được mô tả cho Escherichia coli. Đối với các thí nghiệm MS sử dụng protein tái tổ hợp, chẳng hạn như các thí nghiệm phổ khối cấu trúc (phổ khối trao đổi hydro-deuterium (HDX-MS), liên kết chéo hóa học và dấu vết gốc tự do), việc không đưa các tạp chất này vào cơ sở dữ liệu tìm kiếm trong giai đoạn nhận dạng MS/MS song song ban đầu có thể dẫn đến các biến chứng trong việc nhận dạng peptide. Chúng tôi đã tạo cơ sở dữ liệu FASTA tạp chất cho các chiến lược làm sạch Sf9 và E. coli NiNTA hoặc GST và cho thấy rằng việc sử dụng các cơ sở dữ liệu này có thể cải thiện hiệu quả độ phủ protein HDX-MS, số lượng đoạn và độ tin cậy trong nhận dạng peptide. Phương pháp tiếp cận này cung cấp một chiến lược mạnh mẽ để thiết kế cơ sở dữ liệu tạp chất cho bất kỳ phương pháp làm sạch nào sẽ mở rộng tính phức tạp của các hệ thống có thể được thẩm vấn bằng HDX-MS."} +{"idx": 1870364, "en": "The recent advances of tetramethylpyrazine nitrones and quinolylnitrones for the treatment of stroke have been reviewed and compared with other agents, showing promising therapeutic applications. As a result of a functional transformation of natural product ligustrazine, (Z)-N-tert-butyl-1-(3,5,6-trimethylpyrazin-2-yl)methanimine oxide (6) is a multitarget small nitrone showing potent thrombolytic activity and free radicals scavenging power, in addition to nontoxicity and blood-brain barrier permeability. Similarly, antioxidant (Z)-N-tert-butyl-1-(2-chloro-6-methoxyquinolin-3-yl)methanimine oxide (17) is a novel agent for cerebral ischemia therapy as it is able to scavenge different types of free radical species, showing strong neuroprotection and reduced infarct size.", "gemini": "Những tiến bộ gần đây của tetramethylpyrazine nitrone và quinolylnitrone trong điều trị đột quỵ đã được xem xét và so sánh với các tác nhân khác, cho thấy các ứng dụng điều trị đầy hứa hẹn. Là kết quả của quá trình chuyển đổi chức năng của sản phẩm tự nhiên ligustrazine, (Z)-N-tert-butyl-1-(3,5,6-trimethylpyrazin-2-yl)methanimine oxide (6) là một nitrone nhỏ đa mục tiêu cho thấy hoạt tính tiêu huyết mạnh và sức mạnh dọn gốc tự do, ngoài ra còn có tính không độc hại và tính thấm qua hàng rào máu não. Tương tự như vậy, chất chống oxy hóa (Z)-N-tert-butyl-1-(2-chloro-6-methoxyquinolin-3-yl)methanimine oxide (17) là một tác nhân mới để điều trị thiếu máu cục bộ não vì nó có khả năng dọn sạch các loại gốc tự do khác nhau, cho thấy khả năng bảo vệ thần kinh mạnh mẽ và giảm kích thước nhồi máu."} +{"idx": 1870365, "en": "The dual emission produced from Mn2+ when codoped with rare earth ions like Eu2+ or Ce3+ in inorganic compounds makes these materials attractive as efficient, color-tunable phosphors for warm-white solid-state lighting. Here, a series of efficient blue-green-emitting BaMgSi4O10:Eu2+,Mn2+ phosphors with thermally robust, tunable luminescence are reported. Steady-state and time-resolved photoluminescence spectroscopy reveal that Eu2+ and Mn2+ each occupy a single crystallographic site and confirm that energy transfer occurs from Eu2+ to Mn2+. The internal and external quantum efficiency of BaMgSi4O10:Eu2+,Mn2+ can reach as high as 69.0 and 47.5%, respectively, upon 360 nm excitation. Moreover, this phosphor possesses nearly zero-thermal quenching up to 440 K due to thermally induced electron detrapping. A fabricated UV-excited white LED device incorporating the blue-green-emitting BaMgSi4O10:Eu2+,Mn2+ and the red-emitting Sr2Si5N8:Eu2+ phosphors exhibits an excellent CRI of 94.3 with a correlated color temperature of 3967 K. These results prove the potential applications of Eu2+,Mn2+ codoped BaMgSi4O10 phosphor for generating warm-white light.", "gemini": "Phát xạ kép được tạo ra từ Mn2+ khi đồng pha tạp với các ion đất hiếm như Eu2+ hoặc Ce3+ trong các hợp chất vô cơ làm cho các vật liệu này trở nên hấp dẫn như các chất phát quang hiệu quả, có thể điều chỉnh màu sắc cho đèn trạng thái rắn màu trắng ấm. Tại đây, một loạt các chất phát quang BaMgSi4O10:Eu2+,Mn2+ phát ra màu xanh lục lam hiệu quả với độ phát quang có thể điều chỉnh, mạnh mẽ về mặt nhiệt được báo cáo. Phổ phát quang ổn định trạng thái và phổ phát quang phân giải thời gian cho thấy Eu2+ và Mn2+ mỗi ion chiếm một vị trí tinh thể đơn và xác nhận rằng sự truyền năng lượng xảy ra từ Eu2+ đến Mn2+. Hiệu suất lượng tử bên trong và bên ngoài của BaMgSi4O10:Eu2+,Mn2+ có thể đạt tới 69,0 và 47,5% tương ứng, khi kích thích ở 360 nm. Hơn nữa, chất phát quang này gần như không bị dập tắt nhiệt độ lên đến 440 K do quá trình giải bẫy electron gây ra nhiệt. Một thiết bị đèn LED trắng kích thích bằng tia cực tím chế tạo kết hợp chất phát quang BaMgSi4O10:Eu2+,Mn2+ phát ra màu xanh lục lam và chất phát quang Sr2Si5N8:Eu2+ phát ra màu đỏ cho thấy CRI tuyệt vời là 94,3 với nhiệt độ màu tương quan là 3967 K. Các kết quả này chứng minh các ứng dụng tiềm năng của chất phát quang BaMgSi4O10 pha tạp đồng thời Eu2+,Mn2+ để tạo ra ánh sáng trắng ấm."} +{"idx": 1870366, "en": "Gas-flow assistance is commonly used in ESI-MS for improved transport and desolvation, and fundamental understanding of the underlying phenomena is essential for improvement of aerodynamic interfaces that couple ESI sources and MS. For this purpose, an electrohydrodynamic model is developed for simulation of charged droplet dynamics under the combined effects of gas flow and electric fields with consideration of space charge interactions within the charged aerosol plume. The model is implemented in COMSOL by exploiting a formalism for establishing the droplet trajectories as a sequence of successive droplets ejected at a frequency defined by the electrospray current. The model is used to assess the effect of two distinct flow configurations and compared to the baseline care of electrospray without assist gas. The simulated flows are jet flows oriented coaxially with the ESI spray, with and without imposed vorticity (swirling). Droplet trajectory simulations of a bimodal droplet population consisting of large primary droplets and small progeny droplets reveal a unique capability for vortical assist jet flow to selectively transmit smaller droplets into the MS due to inertial separation. ESI-MS analysis of fluorinated phosphazines subjected to the different gas flow conditions supports the model predictions. The electrohydrodynamic model developed in this work provides a versatile tool to analyze and design aerodynamic ESI interfaces with rigorous incorporation of drag, inertia, and space-charge repulsion and can be used as a powerful simulation methodology for optimizing charged droplet transmission and ultimately improved analytical performance of gas-assisted ESI-MS workflows.", "gemini": "Hỗ trợ dòng khí thường được sử dụng trong ESI-MS để cải thiện vận chuyển và loại bỏ dung môi, và hiểu biết cơ bản về các hiện tượng cơ bản là rất cần thiết để cải thiện các giao diện khí động học kết nối các nguồn ESI và MS. Với mục đích này, một mô hình điện thủy động lực được phát triển để mô phỏng động lực học của các giọt tích điện dưới tác động kết hợp của dòng khí và điện trường với sự cân nhắc về tương tác điện tích không gian trong luồng khí dung tích điện. Mô hình được triển khai trong COMSOL bằng cách khai thác một hình thức để thiết lập các quỹ đạo giọt như một chuỗi các giọt liên tiếp được phun ra ở tần số được xác định bởi dòng điện phun điện. Mô hình được sử dụng để đánh giá hiệu ứng của hai cấu hình dòng chảy riêng biệt và so sánh với dòng chảy cơ bản của phun điện không có khí hỗ trợ. Các dòng chảy được mô phỏng là dòng tia định hướng đồng trục với tia phun ESI, có và không có độ xoáy (xoáy). Mô phỏng quỹ đạo giọt của quần thể giọt hai chế độ bao gồm các giọt sơ cấp lớn và các giọt con nhỏ cho thấy khả năng duy nhất của dòng tia hỗ trợ xoáy để truyền chọn lọc các giọt nhỏ hơn vào MS do tách quán tính. Phân tích ESI-MS về phosphazin được fluorin hóa chịu các điều kiện dòng khí khác nhau hỗ trợ các dự đoán của mô hình. Mô hình điện thủy động lực được phát triển trong công trình này cung cấp một công cụ linh hoạt để phân tích và thiết kế các giao diện ESI khí động lực học với sự kết hợp chặt chẽ giữa lực cản, quán tính và lực đẩy điện tích không gian và có thể được sử dụng như một phương pháp mô phỏng mạnh mẽ để tối ưu hóa truyền giọt tích điện và cuối cùng cải thiện hiệu suất phân tích của quy trình công việc ESI-MS hỗ trợ khí."} +{"idx": 1870367, "en": "The addition of the Grignard 3,4,5-ArFMgBr to aluminum(III) chloride in ether generates the novel triarylalane Al(3,4,5-ArF)3·OEt2. Attempts to synthesize this alane via transmetalation from the parent borane with trimethylaluminum gave a dimeric structure with bridging methyl groups, a product of partial transmetalation. On the other hand, the novel alane Al(2,3,4-ArF)3 was synthesized from the parent borane and trimethylaluminum. Interestingly, the solid-state structure of Al(2,3,4-ArF)3 shows an extended chain structure resulting from neighboring Al···F contacts. Al(3,4,5-ArF)3·OEt2 was then found to be an effective catalyst for the hydroboration of carbonyls, imines, and alkynes with pinacolborane.", "gemini": "Việc bổ sung Grignard 3,4,5-ArFMgBr vào nhôm(III) clorua trong ete tạo ra triarylalane mới Al(3,4,5-ArF)3·OEt2. Các nỗ lực tổng hợp alane này thông qua chuyển kim loại từ boran mẹ với trimethyl nhôm đã tạo ra một cấu trúc dimer với các nhóm metyl nối cầu, một sản phẩm của quá trình chuyển kim loại một phần. Mặt khác, alane mới Al(2,3,4-ArF)3 được tổng hợp từ boran mẹ và trimethyl nhôm. Điều thú vị là cấu trúc trạng thái rắn của Al(2,3,4-ArF)3 cho thấy cấu trúc chuỗi mở rộng là kết quả của các tiếp xúc Al···F lân cận. Sau đó, người ta thấy rằng Al(3,4,5-ArF)3·OEt2 là chất xúc tác hiệu quả cho quá trình hydroborat hóa cacbonyl, imin và ankin với pinacolboran."} +{"idx": 1870368, "en": "Giardia parasites are ubiquitous protozoans of global importance that impact a wide range of animals including humans. They are the most common enteric pathogen of cats and dogs in developed countries and infect ∼1 billion people worldwide. While Giardia infections can be asymptomatic, they often result in severe and chronic diseases. There is also mounting evidence that they are linked to postinfection disorders. Despite growing evidence of the widespread morbidity associated with Giardia infections, current treatment options are limited to compound classes with broad antimicrobial activity. Frontline anti-Giardia drugs are also associated with increasing drug resistance and treatment failures. To improve the health and well-being of millions, new selective anti-Giardia drugs are needed alongside improved health education initiatives. Here we discuss current treatment options together with recent advances and gaps in drug discovery. We also propose criteria to guide the discovery of new anti-Giardia compounds.", "gemini": "Ký sinh trùng Giardia là các loại động vật nguyên sinh phổ biến có tầm quan trọng toàn cầu, ảnh hưởng đến nhiều loại động vật bao gồm cả con người. Chúng là tác nhân gây bệnh đường ruột phổ biến nhất ở mèo và chó ở các nước phát triển và lây nhiễm cho khoảng 1 tỷ người trên toàn thế giới. Trong khi nhiễm trùng Giardia có thể không có triệu chứng, chúng thường dẫn đến các bệnh nghiêm trọng và mãn tính. Cũng có nhiều bằng chứng cho thấy chúng liên quan đến các rối loạn sau nhiễm trùng. Mặc dù có ngày càng nhiều bằng chứng về tỷ lệ mắc bệnh phổ biến liên quan đến nhiễm trùng Giardia, nhưng các lựa chọn điều trị hiện tại chỉ giới hạn ở các nhóm hợp chất có hoạt tính kháng khuẩn phổ rộng. Các loại thuốc chống Giardia tuyến đầu cũng liên quan đến tình trạng kháng thuốc ngày càng tăng và điều trị thất bại. Để cải thiện sức khỏe và hạnh phúc của hàng triệu người, cần có các loại thuốc chống Giardia mới có chọn lọc cùng với các sáng kiến nâng cao sức khỏe cộng đồng. Tại đây, chúng tôi thảo luận về các lựa chọn điều trị hiện tại cùng với những tiến bộ gần đây và khoảng cách trong khám phá thuốc. Chúng tôi cũng đề xuất các tiêu chí để hướng dẫn việc khám phá các hợp chất chống Giardia mới."} +{"idx": 1870369, "en": "Thioamide substitutions of the peptide backbone have been shown to stabilize therapeutic and imaging peptides toward proteolysis. In order to rationally design thioamide modifications, we have developed a novel Rosetta custom score function to classify thioamide positional effects on proteolysis in substrates of serine and cysteine proteases. Peptides of interest were docked into proteases using the FlexPepDock application in Rosetta. Docked complexes were modified to contain thioamides parametrized through the creation of custom atom types in Rosetta based on ab intio simulations. Thioamide complexes were simulated, and the resultant structural complexes provided features for machine learning classification as the decomposed values of the Rosetta score function. An ensemble, majority voting model was developed to be a robust predictor of previously unpublished thioamide proteolysis holdout data. Theoretical control simulations with pseudo-atoms that modulate only one physical characteristic of the thioamide show differential effects on prediction accuracy by the optimized voting classification model. These pseudo-atom model simulations, as well as statistical analyses of the full thioamide simulations, implicate steric effects on peptide binding as being primarily responsible for thioamide positional effects on proteolytic resistance.", "gemini": "Các thế thioamide của chuỗi xương sống peptide đã được chứng minh là làm ổn định các peptide điều trị và tạo ảnh về sự phân giải protein. Để thiết kế hợp lý các sửa đổi thioamide, chúng tôi đã phát triển một hàm số điểm tùy chỉnh Rosetta mới để phân loại các hiệu ứng vị trí thioamide trên sự phân giải protein trong các chất nền của protease serine và cysteine. Các peptide quan tâm đã được ghép vào protease bằng cách sử dụng ứng dụng FlexPepDock trong Rosetta. Các phức hợp ghép được sửa đổi để chứa các thioamide được tham số hóa thông qua việc tạo các loại nguyên tử tùy chỉnh trong Rosetta dựa trên các mô phỏng ab intio. Các phức hợp thioamide được mô phỏng và các phức hợp cấu trúc kết quả cung cấp các tính năng để phân loại học máy làm các giá trị phân hủy của hàm số điểm Rosetta. Một mô hình bỏ phiếu đa số đã được phát triển để trở thành một công cụ dự đoán mạnh mẽ về dữ liệu giữ lại sự phân giải thioamide chưa được công bố trước đó. Các mô phỏng kiểm soát lý thuyết với các nguyên tử giả chỉ điều chỉnh một đặc tính vật lý của thioamide cho thấy các hiệu ứng khác biệt về độ chính xác dự đoán của mô hình phân loại bỏ phiếu được tối ưu hóa. Các mô phỏng mô hình nguyên tử giả này, cũng như các phân tích thống kê về các mô phỏng thioamide đầy đủ, chỉ ra các hiệu ứng lập thể trên liên kết peptide là nguyên nhân chính gây ra các hiệu ứng vị trí thioamide trên khả năng chống phân giải protein."} +{"idx": 1870370, "en": "By establishing the procedures for sequential deprotections, reaction monitoring, purification, and handling, for the first time, we achieved the total synthesis of the proposed structure for protoaculeine B (2), which is a highly hydrophilic and polycationic amino acid. The NMR and mass spectra and chemical reactivity of the synthetic sample differed from those of natural protoaculeine B, which indicates the necessity for revision of the originally reported structure.", "gemini": "Bằng cách thiết lập các quy trình khử bảo vệ tuần tự, theo dõi phản ứng, tinh chế và xử lý, lần đầu tiên chúng tôi đã tổng hợp hoàn toàn cấu trúc được đề xuất cho protoaculeine B (2), đây là một axit amin ưa nước và đa cation. Phổ NMR và khối lượng và phản ứng hóa học của mẫu tổng hợp khác với protoaculeine B tự nhiên, điều này cho thấy sự cần thiết phải sửa đổi cấu trúc được báo cáo ban đầu."} +{"idx": 1870371, "en": "Within the context of a simple avoided crossing, we investigate the effect of a complex-valued diabatic coupling in determining spin-dependent rate constants and scattering states. We find that, if the molecular geometry is not linear and the Berry force is not zero, one can find significant spin polarization of the products. This study emphasizes that, when analyzing nonadiabatic reactions with spin orbit coupling (and a complex-valued Hamiltonian), one must consider how Berry force affects nuclear motion-at least in the context of gas phase reactions. Work is currently ongoing as far as extrapolating these conclusions to the condensed phase, where interesting spin selection has been observed in recent years.", "gemini": "Trong bối cảnh của một giao thoa tránh đơn giản, chúng tôi nghiên cứu hiệu ứng của liên hợp diabatic có giá trị phức tạp trong việc xác định hằng số tốc độ phụ thuộc vào spin và trạng thái tán xạ. Chúng tôi thấy rằng, nếu hình học phân tử không tuyến tính và lực Berry không bằng không, người ta có thể tìm thấy sự phân cực spin đáng kể của các sản phẩm. Nghiên cứu này nhấn mạnh rằng, khi phân tích các phản ứng không đoạn nhiệt với liên hợp quỹ đạo spin (và Hamiltonian có giá trị phức tạp), người ta phải xem xét lực Berry ảnh hưởng đến chuyển động hạt nhân như thế nào - ít nhất là trong bối cảnh phản ứng pha khí. Hiện tại, công việc đang được tiến hành để ngoại suy các kết luận này sang pha ngưng tụ, nơi đã quan sát thấy sự lựa chọn spin thú vị trong những năm gần đây."} +{"idx": 1870372, "en": "Trillium tschonoskii is a medicinal plant known to biosynthesize steroidal saponins. A phytochemical investigation of the rhizomes of T. tschonoskii led to the isolation of nine new furostanol saponins (1-9) and 11 known analogues (10-20). Five of these new compounds were shown to have hydroxy groups at the C-5 and C-6 positions, while two possess a rare aglycone containing carbonyl groups at the C-16 and C-22 positions as well as a Δ17(20) double bond, and the others have conjugated double bonds in the E-ring or have different sugar chains at the C-3 position. All the isolates were tested for their effect on the expansion of human cord blood (CB) CD34+ hematopoietic stem and progenitor cells. It was found that CB CD34+ cells treated with compounds 6, 7, 9, 10, 14, 15, and 19 showed increased numbers of rigorously phenotype-defined hematopoietic stem cells. Notably, compounds 9, 10, 13, and 14 demonstrated an enhanced ability to increase the percentages and numbers of CB CD34+CD38- cells and multipotential progenitors. The present study is the first to report that furostanol saponins from T. tschonoskii rhizomes can promote hematopoietic stem/progenitor cell (HSPC) expansion.", "gemini": "Trillium tschonoskii là một loại cây thuốc được biết đến với khả năng tổng hợp saponin steroid. Một cuộc điều tra về mặt hóa thực vật đối với thân rễ của T. tschonoskii đã dẫn đến việc phân lập được chín saponin furostanol mới (1-9) và 11 chất tương tự đã biết (10-20). Năm trong số những hợp chất mới này được chứng minh là có nhóm hydroxy ở vị trí C-5 và C-6, trong khi hai hợp chất sở hữu một aglycone hiếm có chứa các nhóm carbonyl ở vị trí C-16 và C-22 cũng như một liên kết đôi Δ17(20), và những hợp chất khác có liên kết đôi liên hợp trong vòng E hoặc có các chuỗi đường khác nhau ở vị trí C-3. Tất cả các chất phân lập đều được thử nghiệm về tác dụng của chúng đối với sự giãn nở của tế bào gốc tạo máu và tế bào tiền thân CD34+ trong máu dây rốn người (CB). Người ta thấy rằng các tế bào CB CD34+ được xử lý bằng các hợp chất 6, 7, 9, 10, 14, 15 và 19 cho thấy số lượng tế bào gốc tạo máu được xác định kiểu hình nghiêm ngặt tăng lên. Đáng chú ý, các hợp chất 9, 10, 13 và 14 đã chứng minh khả năng tăng cường tỷ lệ phần trăm và số lượng tế bào CB CD34+CD38- và các tiền thân đa năng. Nghiên cứu hiện tại là nghiên cứu đầu tiên báo cáo rằng saponin furostanol từ thân rễ T. tschonoskii có thể thúc đẩy sự giãn nở của tế bào gốc/tiền thân tạo máu (HSPC)."} +{"idx": 1870373, "en": "Seven new merosesquiterpenoids, trichothecrotocins D-J (1-7), two new trichothecene sesquiterpenoids, trichothecrotocins K (12) and L (13), and six known compounds (8-11, 14, and 15), were isolated from a potato-associated fungus, Trichothecium crotocinigenum. Compounds 5 and 6 were racemates which were further separated as pure enantiomers. Structures together with absolute configurations were established by extensive spectroscopic analysis, as well as quantum chemistry calculations on ECD and optical rotations. Compounds 1-4 are rare meroterpenoids featuring a seco-phenyl group, while 1 and 2 possessed a novel 6-6/5 fused ring system. Compounds 1-4, 8, 11, and 12 showed antifungal activity against four plant pathogens with MIC values of 8-128 μg/mL. It is suggested that the meroterpenoids produced by T. crotocinigenum may play an important role in the antifungal property of the fungus, thereby protecting the host plant, i.e., potato.", "gemini": "Bảy merosesquiterpenoid mới, trichothecrotocin D-J (1-7), hai sesquiterpenoid trichothecene mới, trichothecrotocin K (12) và L (13), và sáu hợp chất đã biết (8-11, 14 và 15), đã được phân lập từ một loại nấm liên quan đến khoai tây, Trichothecium crotocinigenum. Các hợp chất 5 và 6 là các racemat sau đó được tách riêng thành các enantiomer tinh khiết. Cấu trúc cùng với cấu hình tuyệt đối được thiết lập bằng phân tích quang phổ mở rộng, cũng như các tính toán hóa học lượng tử về ECD và độ quay quang. Các hợp chất 1-4 là meroterpenoid hiếm có nhóm seco-phenyl, trong khi 1 và 2 sở hữu hệ thống vòng hợp nhất 6-6/5 mới. Các hợp chất 1-4, 8, 11 và 12 cho thấy hoạt tính kháng nấm đối với bốn loại mầm bệnh thực vật với giá trị MIC từ 8-128 μg/mL. Người ta cho rằng các meroterpenoid do T. crotocinigenum tạo ra có thể đóng vai trò quan trọng trong tính chất kháng nấm của nấm, do đó bảo vệ cây chủ, tức là khoai tây."} +{"idx": 1870374, "en": "Polar surfaces of solid oxides are intrinsically unstable and tend to reconstruct due to the diverging electrostatic energy and thus often exhibit unique physical and chemical properties. However, a quantitative description of the restructuring mechanism of these polar surfaces remains challenging. Here we provide an atomic-level picture of the refaceting process that governs the surface polarity compensation of cubic ceria nanoparticles based on the accurate reference data acquired from the well-defined model systems. The combined results from advanced infrared spectroscopy, atomic-resolved transmission electron microscopy, and density functional theory calculations identify a two-step scenario where an initial O-terminated (2 × 2) reconstruction is followed by a severe refaceting via massive mass transport at elevated temperatures to yield {111}-dominated nanopyramids. This significant surface restructuring promotes the redox properties of ceria nanocubes, which account for the enhanced catalytic activity for CO oxidation.", "gemini": "Các bề mặt phân cực của oxit rắn về bản chất là không ổn định và có xu hướng tái cấu trúc do năng lượng tĩnh điện phân kỳ và do đó thường biểu hiện các tính chất vật lý và hóa học độc đáo. Tuy nhiên, mô tả định lượng về cơ chế tái cấu trúc của các bề mặt phân cực này vẫn còn là một thách thức. Tại đây, chúng tôi cung cấp một bức tranh ở cấp độ nguyên tử về quá trình tái tạo mặt phẳng chi phối sự bù trừ độ phân cực bề mặt của các hạt nano ceria hình khối dựa trên dữ liệu tham chiếu chính xác thu được từ các hệ thống mô hình được xác định rõ. Kết quả kết hợp từ quang phổ hồng ngoại tiên tiến, kính hiển vi điện tử truyền qua có độ phân giải nguyên tử và các phép tính lý thuyết hàm mật độ xác định một kịch bản hai bước trong đó tái cấu trúc (2 × 2) kết thúc bằng O ban đầu tiếp theo là tái tạo mặt phẳng nghiêm trọng thông qua quá trình vận chuyển khối lượng lớn ở nhiệt độ cao để tạo ra các kim tự tháp nano do {111} chi phối. Sự tái cấu trúc bề mặt đáng kể này thúc đẩy các tính chất oxy hóa khử của các khối nano ceria, giải thích cho hoạt tính xúc tác tăng cường đối với quá trình oxy hóa CO."} +{"idx": 1870375, "en": "A series of all 12 cis- and trans-cyclopropanecarboxylic acids and cyclopropylamines bearing CH2F, CHF2, and CF3 substituents were synthesized by different methods on a multigram scale. Dissociation constants (pKa) and log P values were measured for the obtained compounds or their derivatives to evaluate the influence of the type and relative position of fluoroalkyl substituents on the acidity and lipophilicity of monofunctionalized cyclopropanes. An analysis of the selected products by X-ray crystallography was carried out to obtain a better insight into the observed differences in physicochemical properties.", "gemini": "Một loạt 12 axit cis- và trans-cyclopropanecarboxylic và cyclopropylamine mang các nhóm thế CH2F, CHF2 và CF3 đã được tổng hợp bằng các phương pháp khác nhau ở quy mô đa gam. Hằng số phân ly (pKa) và giá trị log P đã được đo đối với các hợp chất thu được hoặc các dẫn xuất của chúng để đánh giá ảnh hưởng của loại và vị trí tương đối của các nhóm thế fluoroalkyl lên tính axit và tính ưa dầu của các cyclopropan được chức năng hóa đơn. Phân tích các sản phẩm được chọn bằng phương pháp tinh thể học tia X đã được thực hiện để hiểu rõ hơn về sự khác biệt quan sát được trong các tính chất lý hóa."} +{"idx": 1870376, "en": "Owing to their unique morphology, ultrasmall lateral sizes, and exceptional properties, graphene quantum dots (GQDs) hold great potential in many applications, especially in the fields of electrochemical biosensors, bioimaging, drug delivery, gene delivery, etc. Their biosafety and potential genotoxicity to human and animal cells have been a growing concern in recent years. Especially, the potential DNA damage caused by GQDs is very crucial but still unclear. In this study, the effect of GQDs on DNA damage has been evaluated by a combination of molecular dynamics (MD) simulations and density functional theory. Our results demonstrate that the DNA damaging mechanism of GQDs depends on the size of GQDs. The small GQDs (seven benzene rings) tend to enter into the interior of DNA molecules and cause a DNA base mismatch. The relatively large GQDs (61 benzene rings) tend to adsorb onto the two ends of a DNA molecule and cause DNA unwinding. Due to the strong interaction between guanine (G) and GQDs, the effect of GQDs is much larger on G than on the other three bases (A, C, and T). In addition, the concentration of GQDs could also affect the results of DNA damaging.", "gemini": "Do hình thái độc đáo, kích thước bên siêu nhỏ và các tính chất đặc biệt, các chấm lượng tử graphene (GQDs) có tiềm năng lớn trong nhiều ứng dụng, đặc biệt là trong các lĩnh vực cảm biến sinh điện hóa, hình ảnh sinh học, đưa thuốc, đưa gen, v.v. Tính an toàn sinh học và độc tính di truyền tiềm ẩn của chúng đối với tế bào người và động vật đã trở thành mối quan tâm ngày càng tăng trong những năm gần đây. Đặc biệt, tổn thương DNA tiềm ẩn do GQD gây ra rất quan trọng nhưng vẫn chưa rõ ràng. Trong nghiên cứu này, tác động của GQD đối với tổn thương DNA đã được đánh giá bằng sự kết hợp giữa mô phỏng động lực học phân tử (MD) và lý thuyết hàm mật độ. Kết quả của chúng tôi chứng minh rằng cơ chế gây tổn thương DNA của GQD phụ thuộc vào kích thước của GQD. Các GQD nhỏ (bảy vòng benzen) có xu hướng đi vào bên trong các phân tử DNA và gây ra sự không phù hợp giữa các bazơ DNA. Các GQD tương đối lớn (61 vòng benzen) có xu hướng hấp phụ vào hai đầu của một phân tử DNA và gây ra sự tháo xoắn DNA. Do tương tác mạnh giữa guanine (G) và GQD, tác động của GQD lớn hơn nhiều đối với G so với ba bazơ còn lại (A, C và T). Ngoài ra, nồng độ GQD cũng có thể ảnh hưởng đến kết quả gây tổn thương DNA."} +{"idx": 1870377, "en": "Molecular perovskite materials have recently attracted extraordinary interest from the academic community owing to their excellent multifunctional properties. Nevertheless, although massive efforts have been made, molecular ferroelastics with three-dimensional (3D) perovskite structures are still rare. Herein, we report two 3D organic-inorganic hybrid perovskites [(2-hydroxy-propyl)-tripropyl-ammonium][Mn(dca)3] (1) and [(2-hydroxy-1-methyl-ethyl)-tripropyl-ammonium] [Mn(dca)3] (2) [dca = dicyanamide, N(CN)2]. The different position of the chiral center results in a tremendous difference in the properties. Compound 1 displays only one phase transition; however, intriguingly, 2 has three phase transitions and represents ferroelastic behavior with exceptional two-step switching of quadratic nonlinear optical (NLO) properties. To the best of our knowledge, this is the first molecular ferroelastic with two-step switching of quadratic NLO properties. The results demonstrate that the molecular chiral design works, and this finding opens up a new avenue to designing multifunctional molecular perovskite materials.", "gemini": "Vật liệu perovskite phân tử gần đây đã thu hút sự quan tâm đặc biệt từ cộng đồng học thuật do các tính chất đa chức năng tuyệt vời của chúng. Tuy nhiên, mặc dù đã có những nỗ lực lớn, các vật liệu sắt đàn hồi phân tử có cấu trúc perovskite ba chiều (3D) vẫn còn rất hiếm. Trong bài báo này, chúng tôi báo cáo hai perovskite lai hữu cơ-vô cơ 3D [(2-hydroxy-propyl)-tripropyl-ammonium] [Mn(dca)3] (1) và [(2-hydroxy-1-methyl-ethyl)-tripropyl-ammonium] [Mn(dca)3] (2) [dca = dicyanamide, N(CN)2]. Vị trí khác nhau của trung tâm chiral dẫn đến sự khác biệt rất lớn về tính chất. Hợp chất 1 chỉ hiển thị một chuyển pha; tuy nhiên, điều thú vị là 2 có ba chuyển pha và thể hiện hành vi sắt đàn hồi với sự chuyển mạch hai bước đặc biệt của các tính chất quang phi tuyến (NLO) bậc hai. Theo hiểu biết của chúng tôi, đây là vật liệu sắt đàn hồi phân tử đầu tiên có khả năng chuyển mạch hai bước của các tính chất NLO bậc hai. Các kết quả chứng minh rằng thiết kế chiral phân tử hoạt động và phát hiện này mở ra một hướng đi mới để thiết kế các vật liệu perovskite phân tử đa chức năng."} +{"idx": 1870378, "en": "The relationship of the hierarchical organization of the skeleton with the local electronic and atomic structure of bone is investigated. The Ca 2p photoemission from intact and various arthritis-damaged areas was measured and examined to study site-dependent peculiarities of calcium bonds in subchondral femoral bone. The medial and lateral condyles of the femur resected during total knee arthroplasty were used as samples. The Ca 2p3/2,1/2-1 photoelectron spectra demonstrate the distinct hierarchy-induced deviations of calcium bonds on the proximal side of the samples. It is shown that the apatite calcium bonds dominate in intact area, whereas non-apatite bonds dominate in OA-damaged areas, especially near sclerotic area but not inside it. The site dependence is associated with the interaction of broken collagen molecules with hydroxyapatite nanocrystallites at the cartilage-bone interface. The interplay of biomechanical and biochemical processes is examined, and the restoration of calcium bonds in sclerotic bone is discussed.", "gemini": "Mối quan hệ giữa tổ chức phân cấp của bộ xương với cấu trúc nguyên tử và điện tử cục bộ của xương được nghiên cứu. Quang điện tử Ca 2p từ các vùng bị viêm khớp nguyên vẹn và khác nhau được đo và kiểm tra để nghiên cứu những đặc điểm riêng biệt của liên kết canxi ở xương đùi dưới sụn. Các lồi cầu trong và ngoài của xương đùi được cắt bỏ trong quá trình phẫu thuật thay khớp gối toàn phần được sử dụng làm mẫu. Các quang phổ quang điện tử Ca 2p3/2,1/2-1 chứng minh sự lệch hướng rõ ràng do phân cấp gây ra của các liên kết canxi ở mặt gần của các mẫu. Kết quả cho thấy các liên kết canxi apatit chiếm ưu thế ở vùng nguyên vẹn, trong khi các liên kết không phải apatit chiếm ưu thế ở các vùng bị OA phá hủy, đặc biệt là gần vùng xơ cứng nhưng không phải bên trong vùng đó. Sự phụ thuộc vào vị trí có liên quan đến sự tương tác của các phân tử collagen bị đứt với các tinh thể nano hydroxyapatite tại ranh giới sụn-xương. Sự tương tác của các quá trình cơ học sinh học và sinh hóa được kiểm tra và quá trình phục hồi các liên kết canxi trong xương xơ cứng được thảo luận."} +{"idx": 1870379, "en": "Nylon and maleic anhydride (MAH)-grafted polyolefin-based thin co-extruded multilayer films are widely used in packaging applications encountered in daily life. The molecular structure of the nylon/MAH-grafted polyolefin buried interface and molecular bonding between these two chemically dissimilar layers are thought to play an important role in achieving packaging structures with good adhesion. Here, the molecular bonds present at a nylon/maleic anhydride (MAH)-grafted polyethylene buried interface were systematically examined in situ for the first time using sum frequency generation (SFG) vibrational spectroscopy. The carbonyl stretching frequency region of the SFG spectra of a nylon/MAH-grafted polyethylene buried interface showed the presence of hydrolyzed MAH groups grafted to the polyethylene chain and very low levels of unreacted MAH enriched at the buried interface. The ability of SFG to detect these molecular species at the buried interface yields important understanding of the interfacial molecular structure and provides the basis for subsequent in situ studies of the bonding reaction between the grafted MAH and nylon directly at the interface. This understanding may guide the design of multilayer films with improved properties such as enhanced adhesion between polymer layers. The approach used in this study is general and is applicable to study the molecular characteristics of other buried interfaces of significance, such as buried interfaces involving polymers in solar cells, polymer semiconductors, and batteries. Nylon impact modification is another area of interest where the interaction between the MAH-grafted elastomer and the continuous phase of nylon is important.", "gemini": "Các màng mỏng đa lớp đồng đùn được ghép nylon và polyolefin có gắn maleic anhydride (MAH) được sử dụng rộng rãi trong các ứng dụng đóng gói trong cuộc sống hàng ngày. Cấu trúc phân tử của giao diện chôn vùi polyolefin ghép nylon/MAH và liên kết phân tử giữa hai lớp không giống nhau về mặt hóa học này được cho là đóng vai trò quan trọng trong việc đạt được cấu trúc đóng gói có độ bám dính tốt. Tại đây, các liên kết phân tử có mặt tại giao diện chôn vùi polyethylene ghép nylon/maleic anhydride (MAH) lần đầu tiên được kiểm tra một cách có hệ thống tại chỗ bằng cách sử dụng quang phổ rung tạo tần số tổng (SFG). Vùng tần số kéo giãn carbonyl của phổ SFG của giao diện chôn vùi polyethylene ghép nylon/MAH cho thấy sự hiện diện của các nhóm MAH đã thủy phân được ghép vào chuỗi polyethylene và mức rất thấp MAH chưa phản ứng được làm giàu tại giao diện chôn vùi. Khả năng của SFG trong việc phát hiện các loài phân tử này tại giao diện chôn vùi mang lại sự hiểu biết quan trọng về cấu trúc phân tử giao diện và cung cấp cơ sở cho các nghiên cứu tại chỗ tiếp theo về phản ứng liên kết giữa MAH ghép và nylon trực tiếp tại giao diện. Sự hiểu biết này có thể hướng dẫn thiết kế các màng mỏng đa lớp có các tính chất được cải thiện như độ bám dính tăng cường giữa các lớp polyme. Cách tiếp cận được sử dụng trong nghiên cứu này có tính tổng quát và có thể áp dụng để nghiên cứu các đặc tính phân tử của các giao diện chôn vùi khác có ý nghĩa, chẳng hạn như các giao diện chôn vùi liên quan đến polyme trong pin, chất bán dẫn polyme và pin mặt trời. Sửa đổi va đập nylon là một lĩnh vực quan tâm khác, trong đó sự tương tác giữa chất đàn hồi ghép MAH và pha liên tục của nylon là rất quan trọng."} +{"idx": 1870380, "en": "Sulfoximines have been largely disregarded in medicinal chemistry for a long time. However, recently, they have risen to the apparent level of stardom on the drug discovery scene. Considering the outstanding properties of sulfoximines, this versatile functional group has advanced to implementation in several drug discovery programs. Currently, this fashionable functional group can be found in various hit-to-lead and lead optimization studies in early stages and in several compounds currently in clinical trials. Herein, we review recent developments to demonstrate the scope and limitations of this interesting and versatile functional group in medicinal chemistry and drug discovery.", "gemini": "Sulfoximine đã bị bỏ qua phần lớn trong hóa học dược phẩm trong một thời gian dài. Tuy nhiên, gần đây, chúng đã vươn lên đến cấp độ nổi tiếng rõ ràng trên sân khấu khám phá thuốc. Xem xét các đặc tính nổi bật của sulfoximine, nhóm chức linh hoạt này đã tiến triển để triển khai trong một số chương trình khám phá thuốc. Hiện tại, nhóm chức thời trang này có thể được tìm thấy trong nhiều nghiên cứu tối ưu hóa thuốc chính và thuốc chính trong giai đoạn đầu và trong một số hợp chất hiện đang trong các thử nghiệm lâm sàng. Trong bài này, chúng tôi xem xét các phát triển gần đây để chứng minh phạm vi và giới hạn của nhóm chức năng thú vị và linh hoạt này trong hóa học dược phẩm và khám phá thuốc."} +{"idx": 1870381, "en": "We investigate the effect of functionalization by acid/amine combinations of four aromatic capping ligands on the optoelectronic properties of CH3NH3PbBr3 perovskite quantum dots (PQDs). These include benzoic acid (BA), phenylacetic acid (PAA), benzylamine, and isopropyl benzylamine. We observe that charge transfer efficiency in PQD films comprising BA-ligated samples varies between 12% and 95% as the dot density is tuned from 102 to 105 dots/μm2 but is consistently ∼92% over that entire range for PAA-ligated PQDs. As temperature T decreases, initially, recombination is dominated by bound or trapped excitons, but below 80 K, spectral broadening, accompanied by free excitonic behavior, is observed. Our results indicate enhanced charge delocalization at lower values of T, which reduces the level of exciton confinement and recombination decay rates and underlines the importance of investigating PQD-ligand interactions at a fundamental level given the significant effect minute changes in ligand structures have on the optoelectronic properties of quantum dots.", "gemini": "Chúng tôi nghiên cứu tác động của quá trình chức năng hóa bằng các tổ hợp axit/amin của bốn phối tử che phủ thơm lên các tính chất quang điện tử của các chấm lượng tử perovskite CH3NH3PbBr3 (PQD). Những chất này bao gồm axit benzoic (BA), axit phenylacetic (PAA), benzylamine và isopropyl benzylamine. Chúng tôi quan sát thấy rằng hiệu suất truyền điện tích trong các màng PQD bao gồm các mẫu liên kết BA thay đổi trong khoảng từ 12% đến 95% khi mật độ chấm được điều chỉnh từ 102 đến 105 chấm/μm2 nhưng liên tục ở mức ∼92% trên toàn bộ phạm vi đó đối với các PQD liên kết PAA. Khi nhiệt độ T giảm, ban đầu, sự tái hợp bị chi phối bởi các exciton liên kết hoặc bị giữ lại, nhưng dưới 80 K, sự mở rộng quang phổ, đi kèm với hành vi exciton tự do, được quan sát thấy. Kết quả của chúng tôi chỉ ra sự dịch chuyển điện tích được tăng cường ở các giá trị T thấp hơn, làm giảm mức độ giới hạn exciton và tốc độ phân rã tái hợp và nhấn mạnh tầm quan trọng của việc nghiên cứu các tương tác PQD-ligand ở cấp độ cơ bản do những thay đổi nhỏ về cấu trúc phối tử có tác động đáng kể đến các tính chất quang điện tử của các chấm lượng tử."} +{"idx": 1870382, "en": "We investigate the dynamic adsorption of anionic surfactant C14 - 16 alpha olefin sulfonate on Berea sandstone cores with different surface wettability and redox states under high temperature that represents reservoir conditions. Surfactant adsorption levels are determined by analyzing the effluent history data with a dynamic adsorption model assuming Langmuir isotherm. A variety of analyses, including surface chemistry, ionic composition, and chromatography, is performed. It is found that the surfactant breakthrough in the neutral-wet core is delayed more compared to that in the water-wet core because the deposited crude oil components on the rock surface increase the surfactant adsorption via hydrophobic interactions. As the surfactant adsorption is satisfied, the crude oil components are solubilized by surfactant micelles and some of the adsorbed surfactants are released from the rock surface. The released surfactant dissolves in the flowing surfactant solution, thereby resulting in an overshoot of the produced surfactant concentration with respect to the injection value. Furthermore, under water-wet conditions, changing the surface redox potential from an oxidized to a reduced state decreases the surfactant adsorption level by 40%. We find that the decrease in surfactant adsorption is caused not only by removing the iron oxide but also by changing the calcium concentration after the core restoration process (calcite dissolution and ion exchange as a result of using EDTA). Findings from this study suggest that laboratory surfactant adsorption tests need to be conducted by considering the wettability and redox state of the rock surface while recognizing how core restoration methods could significantly alter the ionic composition during surfactant flooding.", "gemini": "Chúng tôi nghiên cứu sự hấp phụ động của chất hoạt động bề mặt anion C14 - 16 alpha olefin sulfonate trên lõi đá sa thạch Berea có độ thấm ướt bề mặt và trạng thái oxy hóa khử khác nhau dưới nhiệt độ cao đại diện cho điều kiện của hồ chứa. Mức độ hấp phụ chất hoạt động bề mặt được xác định bằng cách phân tích dữ liệu lịch sử nước thải với một mô hình hấp phụ động giả sử đẳng nhiệt Langmuir. Nhiều loại phân tích khác nhau, bao gồm hóa học bề mặt, thành phần ion và sắc ký, được thực hiện. Người ta thấy rằng sự đột phá của chất hoạt động bề mặt trong lõi ướt trung tính bị chậm lại nhiều hơn so với trong lõi ướt nước vì các thành phần dầu thô lắng đọng trên bề mặt đá làm tăng sự hấp phụ chất hoạt động bề mặt thông qua các tương tác kỵ nước. Khi sự hấp phụ chất hoạt động bề mặt được đáp ứng, các thành phần dầu thô được hòa tan bởi các micelle chất hoạt động bề mặt và một số chất hoạt động bề mặt được hấp phụ được giải phóng khỏi bề mặt đá. Chất hoạt động bề mặt được giải phóng hòa tan trong dung dịch chất hoạt động bề mặt đang chảy, do đó dẫn đến sự vượt ngưỡng của nồng độ chất hoạt động bề mặt được sản xuất so với giá trị tiêm. Hơn nữa, trong điều kiện ướt nước, việc thay đổi điện thế oxy hóa khử bề mặt từ trạng thái oxy hóa sang trạng thái khử làm giảm mức độ hấp phụ chất hoạt động bề mặt xuống 40%. Chúng tôi thấy rằng sự giảm hấp phụ chất hoạt động bề mặt không chỉ do loại bỏ oxit sắt mà còn do thay đổi nồng độ canxi sau quá trình phục hồi lõi (hòa tan canxit và trao đổi ion do sử dụng EDTA). Những phát hiện từ nghiên cứu này cho thấy rằng các thử nghiệm hấp phụ chất hoạt động bề mặt trong phòng thí nghiệm cần được tiến hành bằng cách xem xét độ thấm ướt và trạng thái oxy hóa khử của bề mặt đá trong khi nhận ra rằng các phương pháp phục hồi lõi có thể làm thay đổi đáng kể thành phần ion trong quá trình ngập chất hoạt động bề mặt."} +{"idx": 1870383, "en": "Zinagrandinolide E (1, ZGE) is an elemanolide with antinociceptive action isolated from Zinnia grandiflora (Asteraceae), valued in North México and southwestern United States for pain relief. Herein, we report the anti-inflammatory and antiallodynic action of ZGE (1) in carrageenan-induced inflammation and tactile allodynia in mice and in a neuropathic pain model in hyperglycemic mice. Local peripheral administration of ZGE (1-30 μg/paw) induced dose-dependent acute anti-inflammatory and antiallodynic effects. The anti-inflammatory effect was comparable to diclofenac (30 μg/paw). Intrathecal (i.t.) administration of ZGE (30 μg) in acute experiments did not affect carrageenan-induced inflammation but significantly reduced tactile allodynia in a dose-dependent fashion. In long-term experiments (15 or 6 days), using two different scheme treatments (pretreatment or post-treatment), ZGE (3-30 μg/paw) showed antiallodynic but not anti-inflammatory action. Local peripheral (3-30 μg/paw) or intrathecal (3-30 μg) administration of ZGE partially reversed tactile allodynia in hyperglycemic mice, better or comparable, respectively, with those of pregabalin (30 μg/paw or 30 μg i.t.). The effects were dose-dependent. According to the pharmacological tools employed, the anti-inflammatory and antiallodynic activities of ZGE are multitarget; these involve the opioidergic, serotoninergic, and GABAergic systems, as well as the NO-cGMP-ATP-sensitive K+ channel signaling pathway.", "gemini": "Zinagrandinolide E (1, ZGE) là một elemanolide có tác dụng giảm đau được phân lập từ Zinnia grandiflora (Asteraceae), được đánh giá cao ở miền Bắc México và tây nam Hoa Kỳ để giảm đau. Trong bài báo này, chúng tôi báo cáo tác dụng chống viêm và chống tăng cảm giác đau của ZGE (1) trong tình trạng viêm do carrageenan gây ra và tăng cảm giác đau xúc giác ở chuột và trong mô hình đau thần kinh ở chuột tăng đường huyết. Việc dùng ZGE tại chỗ ngoại vi (1-30 μg/bàn chân) gây ra các tác dụng chống viêm cấp tính và chống tăng cảm giác đau theo liều phụ thuộc. Tác dụng chống viêm tương đương với diclofenac (30 μg/bàn chân). Việc tiêm nội tủy (i.t.) ZGE (30 μg) trong các thí nghiệm cấp tính không ảnh hưởng đến tình trạng viêm do carrageenan gây ra nhưng làm giảm đáng kể tình trạng tăng cảm giác đau xúc giác theo cách phụ thuộc vào liều. Trong các thí nghiệm dài hạn (15 hoặc 6 ngày), sử dụng hai phương pháp điều trị khác nhau (điều trị trước hoặc điều trị sau), ZGE (3-30 μg/bàn chân) cho thấy tác dụng chống tăng cảm giác đau nhưng không có tác dụng chống viêm. Việc dùng ZGE tại chỗ ngoại vi (3-30 μg/bàn chân) hoặc tiêm nội tủy (3-30 μg) làm đảo ngược một phần tình trạng tăng cảm giác đau xúc giác ở chuột tăng đường huyết, tốt hơn hoặc tương đương với pregabalin (30 μg/bàn chân hoặc 30 μg i.t.). Các tác dụng phụ thuộc vào liều. Theo các công cụ dược lý được sử dụng, các hoạt động chống viêm và chống tăng cảm giác đau của ZGE là đa mục tiêu; những hoạt động này liên quan đến các hệ thống opioid, serotonin và GABAergic, cũng như con đường truyền tín hiệu kênh K+ nhạy cảm với NO-cGMP-ATP."} +{"idx": 1870384, "en": "The development of a novel selective synthesis of 3-amino-2H-indazoles from readily available 2-halobenzonitriles is presented. The reaction proceeds through a domino reaction sequence, consisting of a regioselective palladium-catalyzed coupling of monosubstituted hydrazines with 2-halobenzonitriles, followed by an intramolecular hydroamination through a 5-exo-dig cyclization and subsequent isomerization to directly afford a wide variety of substituted 2H-indazole analogues in good to excellent yields.", "gemini": "Bài báo trình bày sự phát triển của một phương pháp tổng hợp chọn lọc mới của 3-amino-2H-indazole từ 2-halobenzonitrile dễ kiếm. Phản ứng diễn ra thông qua một chuỗi phản ứng domino, bao gồm liên kết xúc tác palađi chọn vùng của hydrazine monosubstituted với 2-halobenzonitrile, tiếp theo là phản ứng hydroamin hóa phân tử trong thông qua quá trình vòng hóa 5-exo-dig và quá trình đồng phân hóa tiếp theo để tạo trực tiếp nhiều loại chất tương tự 2H-indazole đã thay thế với hiệu suất tốt đến tuyệt vời."} +{"idx": 1870385, "en": "In situ quantitative measurements of neurotransmitter activities can provide useful insights into the underlying mechanisms of stem cell differentiation, the formation of neuronal networks, and neurodegenerative diseases. Currently, neurotransmitter detection methods suffer from poor spatial resolution, nonspecific detection, and a lack of in situ analysis. To address this challenge, herein, we first developed a graphene oxide (GO)-hybrid nanosurface-enhanced Raman scattering (SERS) array to detect dopamine (DA) in a selective and sensitive manner. Using the GO-hybrid nano-SERS array, we successfully measured a wide range of DA concentrations (10-4 to 10-9 M) rapidly and reliably. Moreover, the measurement of DA from differentiating neural stem cells applies to the characterization of neuronal differentiation. Given the challenges of in situ detection of neurotransmitters at the single-cell level, our developed SERS-based detection method can represent a unique tool for investigating single-cell signaling pathways associated with DA, or other neurotransmitters, and their roles in neurological processes.", "gemini": "Các phép đo định lượng tại chỗ về hoạt động của chất dẫn truyền thần kinh có thể cung cấp thông tin hữu ích về các cơ chế cơ bản của sự biệt hóa tế bào gốc, sự hình thành của các mạng lưới thần kinh và các bệnh thoái hóa thần kinh. Hiện nay, các phương pháp phát hiện chất dẫn truyền thần kinh gặp phải tình trạng độ phân giải không gian kém, phát hiện không đặc hiệu và thiếu phân tích tại chỗ. Để giải quyết thách thức này, tại đây, chúng tôi đầu tiên đã phát triển một mảng tán xạ Raman tăng cường bề mặt nano lai graphene oxide (GO) (SERS) để phát hiện dopamine (DA) theo cách chọn lọc và nhạy cảm. Sử dụng mảng nano-SERS lai GO, chúng tôi đã đo thành công một loạt các nồng độ DA (10-4 đến 10-9 M) một cách nhanh chóng và đáng tin cậy. Hơn nữa, việc đo DA từ tế bào gốc thần kinh biệt hóa áp dụng cho việc mô tả đặc điểm biệt hóa của tế bào thần kinh. Với những thách thức của việc phát hiện tại chỗ các chất dẫn truyền thần kinh ở cấp độ tế bào đơn lẻ, phương pháp phát hiện dựa trên SERS mà chúng tôi phát triển có thể đại diện cho một công cụ độc đáo để nghiên cứu các con đường truyền tín hiệu tế bào đơn liên quan đến DA hoặc các chất dẫn truyền thần kinh khác và vai trò của chúng trong các quá trình thần kinh."} +{"idx": 1870386, "en": "α,α-Dihalo-N-arylacetamides are commonly used as intermediates in various organic reactions. In the study described here, a catalytic synthesis of α,α-dihalo-N-arylacetamides from β-oxo amides was developed using zwitterionic catalysts and N-halosuccinimides as the halogen sources. The corresponding α,α-dihalo-N-arylacetamides were obtained in good to excellent yields, and no aromatic halogenated side products were detected. The reaction conditions were mild, and no strong base or acid was required.", "gemini": "α,α-Dihalo-N-arylacetamide thường được sử dụng làm chất trung gian trong nhiều phản ứng hữu cơ khác nhau. Trong nghiên cứu được mô tả ở đây, một phương pháp tổng hợp xúc tác α,α-dihalo-N-arylacetamide từ β-oxo amide đã được phát triển bằng cách sử dụng chất xúc tác lưỡng tính và N-halosuccinimide làm nguồn halogen. Các α,α-dihalo-N-arylacetamide tương ứng thu được với hiệu suất tốt đến tuyệt vời và không phát hiện thấy sản phẩm phụ halogen hóa thơm. Điều kiện phản ứng nhẹ và không cần axit hoặc bazơ mạnh."} +{"idx": 1870387, "en": "We explore the ultrafast optical response of graphene subjected to intense (∼106 V/cm) local (∼10 nm) electric fields. Nanoscale gating of graphene is achieved using a voltage-biased, SrTiO3-based conductive nanowire junction \"written\" directly under the graphene and isolated from it by an insulating ultrathin (<2 nm) LaAlO3 barrier. Upon illumination with ultrafast visible-to-near-infrared (VIS-NIR) light pulses, the local field from the nanojunction creates a strong gate-tunable second-order nonlinearity in the graphene and produces a substantial difference-frequency (DFG) and sum-frequency generation (SFG) response detected by the nanojunction. Spectrally sharp, gate-tunable extinction features (>99.9%) are observed in the VIS-NIR and SFG spectral ranges, in parameter regimes that are positively correlated with the enhanced nonlinear response. The observed graphene-light interaction and nonlinear response are of fundamental interest and open the way for future exploitation in graphene-based optical devices such as phase shifters, modulators, and nanoscale THz sources.", "gemini": "Chúng tôi khám phá phản ứng quang học cực nhanh của graphene chịu tác động của trường điện cục bộ mạnh (∼106 V/cm) (∼10 nm). Cổng nanoscale của graphene đạt được bằng cách sử dụng một mối nối nanowire dẫn điện SrTiO3 có điện áp ngược \"được viết\" trực tiếp bên dưới graphene và được cách ly khỏi nó bằng một rào cản LaAlO3 siêu mỏng cách điện (<2 nm). Khi chiếu sáng bằng các xung ánh sáng khả kiến-gần hồng ngoại (VIS-NIR) cực nhanh, trường cục bộ từ nanojunction tạo ra một phi tuyến tính bậc hai có thể điều chỉnh cổng mạnh trong graphene và tạo ra một sự khác biệt đáng kể về tần số (DFG) và phản ứng tạo tần số tổng (SFG) được phát hiện bởi nanojunction. Các đặc điểm tiêu diệt có thể điều chỉnh cổng, sắc nét về mặt phổ (>99,9%) được quan sát thấy trong các dải phổ VIS-NIR và SFG, trong các chế độ tham số có mối tương quan tích cực với phản ứng phi tuyến tính tăng cường. Tương tác graphene-ánh sáng và phản ứng phi tuyến tính quan sát được có mối quan tâm cơ bản và mở đường cho việc khai thác trong tương lai trong các thiết bị quang học dựa trên graphene như bộ dịch pha, bộ điều biến và nguồn THz ở cấp độ nano."} +{"idx": 1870388, "en": "Iron-catalyzed alkylative cyclization of alkenes bearing oxygen nucleophiles with secondary and tertiary alkyl bromides through carbon-carbon and carbon-oxygen bond formations has been developed. A broad substrate scope is an attractive feature of this synthetic method, providing a variety of potentially bioactive five- and six-membered oxygen-containing heterocycles. The reaction pathway is proposed to involve a radical addition of the in situ-formed alkyl radical to an alkene followed by carbon-oxygen bond-forming intramolecular cyclization.", "gemini": "Phản ứng vòng hóa alkyl hóa do sắt xúc tác của các anken mang các chất ái nhân hạt nhân oxy với các alkyl bromide bậc hai và bậc ba thông qua sự hình thành liên kết cacbon-cacbon và liên kết cacbon-oxy đã được phát triển. Phạm vi chất nền rộng là một đặc điểm hấp dẫn của phương pháp tổng hợp này, cung cấp nhiều loại vòng tạp dị chứa oxy năm và sáu thành viên có khả năng hoạt tính sinh học. Con đường phản ứng được đề xuất bao gồm quá trình cộng gốc của gốc alkyl được hình thành tại chỗ vào một anken tiếp theo là quá trình vòng hóa nội phân tử tạo liên kết cacbon-oxy."} +{"idx": 1870389, "en": "We report an efficient synthesis of furopyrans through a gold(I)-catalyzed domino reaction. By starting from the same source and changing the solvent of the reaction, two classes of furopyrans are accessible. During this one-step process, which takes place in DMF, two bonds and two heterocycles are formed. DFT calculations furnish the mechanistic understanding of this transformation. The sequence includes a 5-endo-dig cyclization, a regioselective 8-endo-dig cyclization, and a retro 8π and a 6π electrocyclization.", "gemini": "Chúng tôi báo cáo một phương pháp tổng hợp hiệu quả các furopiran thông qua phản ứng domino do vàng(I) xúc tác. Bằng cách bắt đầu từ cùng một nguồn và thay đổi dung môi của phản ứng, hai loại furopiran có thể tiếp cận được. Trong quá trình một bước này, diễn ra trong DMF, hai liên kết và hai vòng tạp dị được hình thành. Các tính toán DFT cung cấp sự hiểu biết về cơ chế của quá trình chuyển đổi này. Trình tự bao gồm một vòng hóa 5-endo-dig, một vòng hóa 8-endo-dig theo vùng và một vòng điện tử 8π và 6π ngược."} +{"idx": 1870390, "en": "The field of artificial intelligence (AI) is transforming almost every aspect of modern society, including medical imaging. In computed tomography (CT), AI holds the promise of enabling further reductions in patient radiation dose through automation and optimisation of data acquisition processes, including patient positioning and acquisition parameter settings. Subsequent to data collection, optimisation of image reconstruction parameters, advanced reconstruction algorithms, and image denoising methods improve several aspects of image quality, especially in reducing image noise and enabling the use of lower radiation doses for data acquisition. Finally, AI-based methods to automatically segment organs or detect and characterise pathology have been translated out of the research environment and into clinical practice to bring automation, increased sensitivity, and new clinical applications to patient care, ultimately increasing the benefit to the patient from medically justified CT examinations. In summary, since the introduction of CT, a large number of technical advances have enabled increased clinical benefit and decreased patient risk, not only by reducing radiation dose, but also by reducing the likelihood of errors in the performance and interpretation of medically justified CT examinations.", "gemini": "Lĩnh vực trí tuệ nhân tạo (AI) đang chuyển đổi hầu hết mọi khía cạnh của xã hội hiện đại, bao gồm cả hình ảnh y tế. Trong chụp cắt lớp vi tính (CT), AI hứa hẹn cho phép giảm tiếp liều bức xạ cho bệnh nhân thông qua tự động hóa và tối ưu hóa các quy trình thu thập dữ liệu, bao gồm định vị bệnh nhân và cài đặt thông số thu nhận. Sau khi thu thập dữ liệu, việc tối ưu hóa các thông số tái tạo hình ảnh, các thuật toán tái tạo tiên tiến và các phương pháp khử nhiễu hình ảnh cải thiện một số khía cạnh của chất lượng hình ảnh, đặc biệt là trong việc giảm nhiễu hình ảnh và cho phép sử dụng liều bức xạ thấp hơn để thu thập dữ liệu. Cuối cùng, các phương pháp dựa trên AI để tự động phân đoạn các cơ quan hoặc phát hiện và mô tả đặc điểm bệnh lý đã được chuyển ra khỏi môi trường nghiên cứu và vào thực hành lâm sàng để mang lại tính tự động, tăng độ nhạy và các ứng dụng lâm sàng mới vào việc chăm sóc bệnh nhân, cuối cùng là tăng lợi ích cho bệnh nhân từ các kỳ thi CT có lý do về mặt y tế. Tóm lại, kể từ khi CT ra đời, một số lượng lớn các tiến bộ kỹ thuật đã cho phép tăng lợi ích lâm sàng và giảm rủi ro cho bệnh nhân, không chỉ bằng cách giảm liều bức xạ mà còn bằng cách giảm khả năng xảy ra lỗi trong quá trình thực hiện và giải thích các kỳ thi CT có lý do về mặt y tế."} +{"idx": 1870391, "en": "OBJECTIVE Given the lack of information on the psychological impact of COVID-19 on people aged ≥60, we aimed to describe their psychological responses to this pandemic and lockdown situation and compare them with those under 60 years of age. METHODS Secondary analysis of a larger online cross-sectional study designed to determine the psychological impact of the COVID-19 pandemic and lockdown across Spain. We analyzed a total of 1690 respondents aged ≥60 years and compared them with 13,363 respondents under 60 years of age. We employed the Depression, Anxiety, and Stress Scale and the Impact of Event Scale to evaluate psychological responses. RESULTS In all, 52.6% of women and 34.3% of men were found to be probable cases of any emotional distress (p < 0.001). In both sexes, the most common psychological response was avoidance behavior (34.7% and 23.8%, respectively), followed by depression (28.5 and 14.2%). Older women and men were considered probable cases of any emotional distress less often than younger ones (wom52.6% vs. 72.3%, p < 0.001; m34.3% vs. 50.6%, p < 0.001). Finally, the results of the binary logistic regression showed that only depressive and stress responses are psychological factors associated with age group [age ≥ 60 years, O.R. = 0.617 (95% CI = 0.501 - 0.759) and 0.437 (95% CI = 0.334 - 0.573), respectively]. CONCLUSION Contrary to our hypothesis and despite the high percentage of emotional distress we found in older adults, especially women, they are actually at lower risk of developing depressive and stress consequences from COVID-19 and lockdown than those under 60 years of age. That said, we believe our results highlight the need for expert guidance in this age group, especially older women living alone.", "gemini": "MỤC TIÊU Do thiếu thông tin về tác động tâm lý của COVID-19 đối với những người từ 60 tuổi trở lên, chúng tôi đặt mục tiêu mô tả các phản ứng tâm lý của họ đối với đại dịch và tình trạng phong tỏa này và so sánh họ với những người dưới 60 tuổi. PHƯƠNG PHÁP Phân tích thứ cấp của một nghiên cứu cắt ngang trực tuyến lớn hơn được thiết kế để xác định tác động tâm lý của đại dịch COVID-19 và tình trạng phong tỏa trên khắp Tây Ban Nha. Chúng tôi đã phân tích tổng số 1690 người trả lời từ 60 tuổi trở lên và so sánh họ với 13.363 người trả lời dưới 60 tuổi. Chúng tôi sử dụng Thang đo trầm cảm, lo âu và căng thẳng và Thang đo tác động của sự kiện để đánh giá các phản ứng tâm lý. KẾT QUẢ Nhìn chung, 52,6% phụ nữ và 34,3% nam giới được phát hiện là những trường hợp có khả năng gặp bất kỳ tình trạng đau khổ về mặt cảm xúc nào (p <0,001). Ở cả hai giới, phản ứng tâm lý phổ biến nhất là hành vi né tránh (lần lượt là 34,7% và 23,8%), tiếp theo là trầm cảm (28,5 và 14,2%). Phụ nữ và nam giới lớn tuổi được coi là những trường hợp có khả năng gặp bất kỳ tình trạng đau khổ về mặt cảm xúc nào ít hơn những người trẻ tuổi (nữ 52,6% so với 72,3%, p <0,001; nam 34,3% so với 50,6%, p <0,001). Cuối cùng, kết quả của hồi quy logistic nhị phân cho thấy chỉ có các phản ứng trầm cảm và căng thẳng là các yếu tố tâm lý liên quan đến nhóm tuổi [tuổi ≥ 60, O.R. = 0,617 (95% CI = 0,501 - 0,759) và 0,437 (95% CI = 0,334 - 0,573), tương ứng]. KẾT LUẬN Trái ngược với giả thuyết của chúng tôi và mặc dù tỷ lệ đau khổ về mặt cảm xúc cao mà chúng tôi tìm thấy ở người lớn tuổi, đặc biệt là phụ nữ, nhưng trên thực tế, họ có nguy cơ thấp hơn khi phát triển các hậu quả về trầm cảm và căng thẳng do COVID-19 và tình trạng phong tỏa so với những người dưới 60 tuổi. Điều đó nói rằng, chúng tôi tin rằng kết quả của chúng tôi nêu bật nhu cầu hướng dẫn chuyên môn ở nhóm tuổi này, đặc biệt là những phụ nữ lớn tuổi sống một mình."} +{"idx": 1870392, "en": "Optimal treatment for patients with ischemic heart disease and severe left ventricular dysfunction is a debatable subject in the literature. The largest and only trial on the subject so far is the Surgical Treatment for Ischemic Heart Failure trial. This trial compared coronary artery bypass grafting with optimal medical treatment in one arm versus coronary artery bypass grafting with surgical ventricular restoration in the second arm. Recently, the 10-year follow-up data of various subsets of the trial have been published. This study reviews various pertinent clinical issues related to the trial and its sub-studies and their relevance in routine modern-day clinical practice.", "gemini": "Điều trị tối ưu cho những bệnh nhân mắc bệnh tim thiếu máu cục bộ và suy thất trái nặng là một chủ đề gây tranh cãi trong tài liệu. Thử nghiệm lớn nhất và duy nhất về vấn đề này cho đến nay là thử nghiệm Điều trị phẫu thuật cho suy tim do thiếu máu cục bộ. Thử nghiệm này so sánh ghép bắc cầu động mạch vành với điều trị nội khoa tối ưu ở một nhánh so với ghép bắc cầu động mạch vành với phục hồi thất phẫu thuật ở nhánh thứ hai. Gần đây, dữ liệu theo dõi 10 năm của các phân nhóm khác nhau của thử nghiệm đã được công bố. Nghiên cứu này xem xét các vấn đề lâm sàng có liên quan khác nhau liên quan đến thử nghiệm và các nghiên cứu phụ của nó và sự liên quan của chúng trong thực hành lâm sàng hiện đại thường quy."} +{"idx": 1870393, "en": "The biomechanical influence of brace treatment for scoliosis is not understood. This prospective pilot study analyzed reliability of a system based on 100% textile sensors for measurement of pressure exerted by brace on trunk. Evaluation of modifications during changes in the location of the support zones and variations in amplitude of the pressure was observed in four patients, when considering three main daily positions mimicking day and nighttime wear. A calibration step allowed the determination of a conversion law between sensor data and pressure unit using a second order polynomial function with a high r2 of 0.99. This prototype is the first of a new generation. Experimental pressure distribution could be useful for the further brace development.", "gemini": "Ảnh hưởng của nẹp chỉnh hình đối với bệnh vẹo cột sống vẫn chưa được hiểu rõ. Nghiên cứu thí điểm này đã phân tích độ tin cậy của một hệ thống dựa trên cảm biến dệt 100% để đo áp lực do nẹp tác dụng lên thân. Đánh giá các sửa đổi trong quá trình thay đổi vị trí của vùng hỗ trợ và sự thay đổi về biên độ áp lực đã được quan sát ở bốn bệnh nhân, khi xem xét ba tư thế chính hàng ngày mô phỏng mặc ban ngày và ban đêm. Một bước hiệu chuẩn cho phép xác định định luật chuyển đổi giữa dữ liệu cảm biến và đơn vị áp suất bằng cách sử dụng hàm đa thức bậc hai với r2 cao là 0,99. Nguyên mẫu này là sản phẩm đầu tiên của một thế hệ mới. Phân bố áp lực thử nghiệm có thể hữu ích cho quá trình phát triển nẹp thêm."} +{"idx": 1870394, "en": "Background: The term God, included in 5 of the 12 Steps of Narcotics Anonymous (NA) and Alcoholics Anonymous (AA), self-designated spiritual fellowships, has not been studied empirically relative to members' experiences. A greater understanding of this can be clinically useful and can shed light on the 12 Step process of recovery. Objectives: To determine how NA members understand the 12-step concept \"God as we understood Him\" and the relationship between their understanding of God and the intensity of their craving and depressive symptoms.\" Methods: 450 (59% male) NA members completed a survey related to their experiences relative to their relationship with \"God.\" The relationship among these variables and comparisons to the general population was analyzed. Craving and depressive symptoms were assessed by self-report. Results: 98% of the NA participants believe in God explicitly or some other higher power (vs 89% of a probability sample of the US population), 67% believe that God determines what happens to them some or all of the time (vs 48%), 78% (vs 28%) report hearing God talking to them \"in their mind\"; and 37% report that God talks to them \"out loud.\" Acceptance of 12 Step God-related variables inversely predicted a significant portion of the variance of scores on craving (7.5%) and depression (13.5%). Conclusions: Respondents' understanding of God in NA varied considerably and was predictive of their depressive symptoms and craving intensity. These findings can serve as a basis for research into mechanisms underlying NA/AA recovery experiences and can also aid clinicians in how to employ these programs.", "gemini": "Bối cảnh: Thuật ngữ Chúa, được đưa vào 5 trong 12 Bước của Hội Những người nghiện ma túy ẩn danh (NA) và Hội Những người nghiện rượu ẩn danh (AA), các nhóm tự xưng là nhóm tâm linh, chưa được nghiên cứu thực nghiệm liên quan đến kinh nghiệm của các thành viên. Hiểu biết sâu hơn về điều này có thể hữu ích về mặt lâm sàng và có thể làm sáng tỏ quá trình phục hồi 12 Bước. Mục tiêu: Xác định cách các thành viên NA hiểu khái niệm 12 bước \"Chúa như chúng ta hiểu Ngài\" và mối quan hệ giữa sự hiểu biết của họ về Chúa và mức độ thèm muốn và các triệu chứng trầm cảm của họ. Phương pháp: 450 (59% nam) thành viên NA đã hoàn thành một cuộc khảo sát liên quan đến kinh nghiệm của họ liên quan đến mối quan hệ của họ với \"Chúa\". Mối quan hệ giữa các biến này và so sánh với dân số chung đã được phân tích. Các triệu chứng thèm muốn và trầm cảm được đánh giá bằng cách tự báo cáo. Kết quả: 98% những người tham gia NA tin vào Chúa một cách rõ ràng hoặc một sức mạnh siêu nhiên nào khác (so với 89% mẫu xác suất của dân số Hoa Kỳ), 67% tin rằng Chúa quyết định những gì xảy ra với họ một phần hoặc toàn bộ thời gian (so với 48%), 78% (so với 28%) báo cáo rằng họ nghe Chúa nói chuyện với họ \"trong tâm trí của họ\"; và 37% báo cáo rằng Chúa nói chuyện với họ \"bằng lời nói\". Việc chấp nhận các biến liên quan đến Chúa trong 12 Bước dự đoán ngược lại một phần đáng kể của phương sai điểm số về thèm muốn (7,5%) và trầm cảm (13,5%). Kết luận: Sự hiểu biết của những người trả lời về Chúa trong NA rất khác nhau và có thể dự đoán các triệu chứng trầm cảm và mức độ thèm muốn của họ. Những phát hiện này có thể làm cơ sở cho nghiên cứu về các cơ chế cơ bản của các kinh nghiệm phục hồi NA/AA và cũng có thể giúp các bác sĩ lâm sàng cách sử dụng các chương trình này như thế nào."} +{"idx": 1870395, "en": "Mutations in SYNE1 have been originally described to cause a slowly progressive, pure cerebellar ataxia (spinocerebellar ataxia, autosomal-recessive 8; SCAR8). Notably, recent studies revealed that affected patients with SYNE1-associated ataxia can present with complex phenotypes rather than pure cerebellar ataxia, including motor neuron and brainstem dysfunctions. We herein report a Japanese patient diagnosed with juvenile amyotrophic lateral sclerosis (ALS) with a complex phenotype, who carried compound heterozygous pathogenic variants in SYNE1. Of the variants, one was a novel frameshift variant and the other was a nonsense variant previously reported as pathogenic for SCAR8. The patient showed an early age at onset with a relatively slow but progressive course of ALS, accompanied by cognitive decline. Our findings suggest that the clinical spectrum of patients carrying pathogenic SYNE1 variants is broader than expected, and SYNE1 variants should be considered in patients diagnosed with juvenile ALS, even without prominent cerebellar ataxia.", "gemini": "Các đột biến trong SYNE1 ban đầu được mô tả là nguyên nhân gây ra chứng mất điều hòa tiểu não tiến triển chậm, thuần túy (mất điều hòa tiểu não, lặn tính trạng trội trên nhiễm sắc thể thường 8; SCAR8). Đáng chú ý, các nghiên cứu gần đây cho thấy những bệnh nhân bị ảnh hưởng bởi chứng mất điều hòa liên quan đến SYNE1 có thể biểu hiện kiểu hình phức tạp chứ không phải chỉ mất điều hòa tiểu não, bao gồm cả rối loạn chức năng thân não và tế bào vận động. Chúng tôi xin báo cáo về một bệnh nhân người Nhật được chẩn đoán mắc bệnh xơ cứng teo cơ bên (ALS) ở trẻ vị thành niên với kiểu hình phức tạp, mang các biến thể gây bệnh dị hợp tử kép trong SYNE1. Trong số các biến thể, một là biến thể dịch khung mới và biến thể còn lại là biến thể vô nghĩa trước đây được báo cáo là gây bệnh đối với SCAR8. Bệnh nhân khởi phát ở độ tuổi sớm với tiến trình ALS tương đối chậm nhưng tiến triển, kèm theo suy giảm nhận thức. Những phát hiện của chúng tôi cho thấy rằng phổ lâm sàng của những bệnh nhân mang các biến thể SYNE1 gây bệnh rộng hơn dự kiến, và các biến thể SYNE1 nên được xem xét ở những bệnh nhân được chẩn đoán mắc ALS ở trẻ vị thành niên, ngay cả khi không có chứng mất điều hòa tiểu não rõ ràng."} +{"idx": 1870396, "en": "Biologic drugs are increasingly being prescribed for the treatment of psoriasis. Very little information is available in the literature regarding potential drug interactions with these medications. This paper serves as a guide for prescribers to be aware of possible interactions between biologic drugs approved for the treatment of psoriasis in North America and concomitant therapies. OBJECTIVE To provide an overview of reported drug interactions between biologic drugs and concomitant therapies. METHODS Reports of potential drug interactions were compiled through a search of Micromedex, drug monographs (Canadian, American, and European), as well as a PubMed search of each biologic drug with the term \"drug interaction.\" CONCLUSION Generally, caution should be exercised when multiple immunosuppressive therapies are prescribed due to increased risk of infection. However, this is more the result of a synergistic effect as opposed to a true drug interaction. There have been cases where multiple biologic therapies have been concomitantly used without adverse events, as their mechanisms involved different pathways. The sources used to compile this guide were often comprised of low levels of evidence, reinforcing the idea that further studies are required to better direct prescribers.", "gemini": "Các thuốc sinh học ngày càng được kê đơn để điều trị bệnh vẩy nến. Có rất ít thông tin có sẵn trong tài liệu về các tương tác thuốc có thể xảy ra với các loại thuốc này. Bài báo này đóng vai trò như một hướng dẫn cho những người kê đơn để nhận biết các tương tác có thể xảy ra giữa các thuốc sinh học được chấp thuận để điều trị bệnh vẩy nến ở Bắc Mỹ và các liệu pháp đi kèm. MỤC TIÊU Cung cấp tổng quan về các tương tác thuốc được báo cáo giữa các thuốc sinh học và các liệu pháp đi kèm. PHƯƠNG PHÁP Các báo cáo về các tương tác thuốc có thể xảy ra đã được biên soạn thông qua việc tìm kiếm Micromedex, các chuyên luận về thuốc (Canada, Hoa Kỳ và Châu Âu), cũng như tìm kiếm PubMed về từng loại thuốc sinh học với thuật ngữ \"tương tác thuốc\". KẾT LUẬN Nói chung, cần thận trọng khi kê đơn nhiều liệu pháp ức chế miễn dịch do nguy cơ nhiễm trùng tăng lên. Tuy nhiên, đây là kết quả của hiệu ứng hiệp đồng chứ không phải là tương tác thuốc thực sự. Đã có những trường hợp sử dụng nhiều liệu pháp sinh học đồng thời mà không có tác dụng phụ, vì cơ chế của chúng liên quan đến các con đường khác nhau. Các nguồn được sử dụng để biên soạn hướng dẫn này thường bao gồm các mức độ bằng chứng thấp, củng cố thêm ý tưởng rằng cần có thêm các nghiên cứu để hướng dẫn tốt hơn cho những người kê đơn."} +{"idx": 1870397, "en": "Glioblastoma multiforme (GBM) is the most common and the most malignant primary intracranial tumor in adults. GBM extraneural metastases occur in only approximately 0.2-0.4% of patients. We present a case of a cervical metastasis of glioblastoma after cranial tumor resection. In concord with case presentation, we reviewed the metastatic location and metastasis time of the gliablastomas seen in the literature.", "gemini": "U nguyên bào thần kinh đệm đa hình (GBM) là khối u nội sọ nguyên phát phổ biến nhất và ác tính nhất ở người lớn. Di căn ngoài thần kinh của GBM chỉ xảy ra ở khoảng 0,2-0,4% bệnh nhân. Chúng tôi trình bày một trường hợp di căn cổ tử cung của u nguyên bào thần kinh đệm sau khi cắt bỏ khối u sọ. Phù hợp với trình bày trường hợp, chúng tôi đã xem xét vị trí di căn và thời gian di căn của các u nguyên bào thần kinh đệm được thấy trong tài liệu."} +{"idx": 1870398, "en": "In this report we present a case of missed hypertension due to subclavian artery stenosis. A 77 year-old female patient, initially thought as being normotensive, was referred to us due to newly discovered systolic heart murmur suspicious for aortic stenosis. We noted inter-arm blood pressure difference of 30 mmHg, with higher, hypertensive values on right arm. Further workup and medical imaging excluded aortic stenosis and revealed an asymptomatic, hemodynamically significant, stenosis of left subclavian artery. Due to absence of symptoms, the patient has been managed with conservative therapy for subclavian stenosis and hypertension, and she is currently in good conditions and followed up for any signs of disease progression. This case clearly shows importance of measuring blood pressure on both arms when initially diagnosing hypertension as this is often overlooked and is key to properly diagnose hypertension and possible subclavian stenosis.", "gemini": "Trong báo cáo này, chúng tôi trình bày một trường hợp tăng huyết áp không được phát hiện do hẹp động mạch dưới đòn. Một bệnh nhân nữ 77 tuổi, ban đầu được cho là bình thường, đã được chuyển đến chúng tôi do phát hiện mới tiếng thổi tâm thu nghi ngờ hẹp động mạch chủ. Chúng tôi ghi nhận chênh lệch huyết áp giữa hai tay là 30 mmHg, với các giá trị cao hơn, tăng huyết áp ở cánh tay phải. Việc kiểm tra và chụp ảnh y tế thêm đã loại trừ hẹp động mạch chủ và phát hiện ra hẹp động mạch dưới đòn trái không có triệu chứng, có ý nghĩa về huyết động học. Do không có triệu chứng, bệnh nhân đã được điều trị bảo tồn đối với hẹp động mạch dưới đòn và tăng huyết áp, và hiện tại bệnh nhân trong tình trạng tốt và được theo dõi mọi dấu hiệu tiến triển của bệnh. Trường hợp này cho thấy rõ tầm quan trọng của việc đo huyết áp ở cả hai tay khi chẩn đoán tăng huyết áp ban đầu vì điều này thường bị bỏ qua và là chìa khóa để chẩn đoán đúng tăng huyết áp và hẹp động mạch dưới đòn có thể xảy ra."} +{"idx": 1870399, "en": "PURPOSE To evaluate multifocal evanescent white dot syndrome (MEWDS) patients with and without overlapping multifocal choroiditis (MFC). METHODS Thirty-four patients diagnosed between November 2008 and August 2018 at 2 hospitals were retrospectively reviewed. Multimodal imaging including optical coherence tomography angiography (OCTA) was assessed. RESULTS Twenty-seven (79%) patients were women (overall mean age, 30 years). Five developed MFC lesions in the affected eyes, and one developed MFC in the unaffected fellow eye. Another developed combined MEWDS and MFC in the fellow eye during follow-up. Eyes with MFC had thicker choroids (316.5 and 197.3 µm, respectively; P = .001) at presentation, which became thinner after resolution (P = .043). Those with subfoveal MFC had poor visual prognosis (P = .019). Areas of suspected choriocapillaris flow deficit were identified on OCTA. CONCLUSION Some MEWDS patients may develop overlapping MFC in the same or fellow eyes, presenting with increased choroidal thickness which decreased after resolution.", "gemini": "MỤC ĐÍCH Đánh giá những bệnh nhân mắc hội chứng đốm trắng thoáng qua đa ổ (MEWDS) có và không có viêm màng mạch đa ổ (MFC) chồng lấn. PHƯƠNG PHÁP Ba mươi bốn bệnh nhân được chẩn đoán từ tháng 11 năm 2008 đến tháng 8 năm 2018 tại 2 bệnh viện đã được xem xét lại hồi cứu. Đã đánh giá hình ảnh đa phương thức bao gồm chụp mạch máu học quang học (OCTA). KẾT QUẢ Hai mươi bảy (79%) bệnh nhân là phụ nữ (tuổi trung bình chung là 30). Năm bệnh nhân phát triển các tổn thương MFC ở mắt bị ảnh hưởng và một bệnh nhân phát triển MFC ở mắt lành không bị ảnh hưởng. Một bệnh nhân khác phát triển MEWDS kết hợp và MFC ở mắt lành trong quá trình theo dõi. Những mắt bị MFC có màng mạch dày hơn (lần lượt là 316,5 và 197,3 µm; P = 0,001) khi khám, sau đó trở nên mỏng hơn sau khi phục hồi (P = 0,043). Những người bị MFC dưới hoàng điểm có tiên lượng thị lực kém (P = 0,019). Các vùng nghi ngờ thiếu lưu lượng mao mạch màng mạch đã được xác định trên OCTA. KẾT LUẬN Một số bệnh nhân MEWDS có thể phát triển MFC chồng lấn ở cùng một mắt hoặc mắt lành, biểu hiện là độ dày của màng mạch tăng lên và giảm sau khi phục hồi."} +{"idx": 1870400, "en": "STUDY OBJECTIVE Emergency Departments (ED) are rapidly becoming an important location for initiation of buprenorphine (EDBUP) for the treatment of opioid use disorder (OUD). Previous investigations of emergency medicine physicians' perceived barriers and attitudes toward EDBUP exclusively sampled from urban, academic-affiliated physicians. We administered a multistate survey to an institutionally and geographically diverse collection of emergency medicine physicians to better understand the professional opinions of EDBUP implementation across a variety of practice settings. METHODS This cross-sectional survey study used an online survey instrument to convenience sample emergency medicine physicians. In order to sample from various practice environments, participants were identified from (1) statewide ACEP chapters and (2) Facebook groups exclusive to emergency medicine physicians. The survey explored physicians' attitudes of EDBUP adoption and the perceived barriers to doing so. RESULTS 162 emergency medicine physicians completed the survey. 76% of respondents agreed that emergency medicine physicians should offer EDBUP in the treatment of OUD. When stratified by practice setting and X-waiver status, 96% of X-waivered physicians, 73% of academic physicians, 49% of non-academic physicians, and 34% of non-X-waivered physicians felt comfortable initiating EDBUP. Lack of access to outpatient MOUD referral was the most frequently cited barrier to EDBUP across all practice settings. CONCLUSIONS An institutionally and geographically diverse group of emergency medicine physicians endorsed substantial support for EDBUP. Emergency medicine physicians practicing in different clinical environments endorsed similar barriers to EDBUP implementation.", "gemini": "MỤC TIÊU NGHIÊN CỨU Các Khoa Cấp cứu (ED) đang nhanh chóng trở thành một địa điểm quan trọng để bắt đầu điều trị bằng buprenorphine (EDBUP) cho chứng rối loạn sử dụng opioid (OUD). Các cuộc điều tra trước đây về các rào cản và thái độ nhận thức của các bác sĩ cấp cứu đối với EDBUP chỉ lấy mẫu từ các bác sĩ đô thị, có liên kết với học viện. Chúng tôi đã tiến hành một cuộc khảo sát đa tiểu bang đối với một nhóm các bác sĩ cấp cứu đa dạng về mặt thể chế và địa lý để hiểu rõ hơn về ý kiến chuyên môn về việc triển khai EDBUP trên nhiều bối cảnh hành nghề. PHƯƠNG PHÁP Nghiên cứu khảo sát cắt ngang này đã sử dụng một công cụ khảo sát trực tuyến để lấy mẫu thuận tiện các bác sĩ cấp cứu. Để lấy mẫu từ các môi trường hành nghề khác nhau, những người tham gia được xác định từ (1) các chi hội ACEP trên toàn tiểu bang và (2) các nhóm Facebook dành riêng cho các bác sĩ cấp cứu. Cuộc khảo sát đã tìm hiểu thái độ của các bác sĩ về việc áp dụng EDBUP và những rào cản nhận thức đối với việc làm như vậy. KẾT QUẢ 162 bác sĩ cấp cứu đã hoàn thành cuộc khảo sát. 76% số người được hỏi đồng ý rằng các bác sĩ cấp cứu nên cung cấp EDBUP trong điều trị OUD. Khi phân tầng theo bối cảnh hành nghề và trạng thái miễn trừ X, 96% bác sĩ được miễn trừ X, 73% bác sĩ học thuật, 49% bác sĩ không phải học thuật và 34% bác sĩ không được miễn trừ X cảm thấy thoải mái khi bắt đầu sử dụng EDBUP. Việc thiếu quyền tiếp cận với dịch vụ chuyển tuyến ngoại trú MOUD là rào cản được trích dẫn nhiều nhất đối với EDBUP trên tất cả các bối cảnh hành nghề. KẾT LUẬN Một nhóm các bác sĩ cấp cứu đa dạng về mặt thể chế và địa lý đã chứng thực sự ủng hộ đáng kể đối với EDBUP. Các bác sĩ cấp cứu hành nghề trong các môi trường lâm sàng khác nhau đã chứng thực những rào cản tương tự đối với việc triển khai EDBUP."} +{"idx": 1870401, "en": "AIM To look at markers to differentiate ocular sarcoidosis from ocular tuberculosis in a high TB endemic population. MATERIALS & METHODS Retrospective multicenter study involving seven tertiary eyecare centers in India between July 2016 to December 2016. Demographic, clinical and laboratory data were retrieved from respective hospital databases. Group A included biopsy-proven ocular sarcoidosis while group B included ocular tuberculosis. RESULTS Data from 2726 consecutive patients with uveitis were retrieved from the databases. Group A had 61 cases while group B included 307 cases. The mean age in group A was 43 ± 16.55 years while in group B was 38 ± 13.13 years. Females were more common in group A. Uveitis with low Schirmer test (OR-30, CI-95%), candlewax retinal vasculitis (OR-8.69, CI-95%), hilar lymphadenopathy (OR-15.23, CI-95%), fissural nodules (OR-39.11, CI-95%) had higher odds of having ocular sarcoidosis. CONCLUSION Presence of dry eye, candlewax retinal vasculitis, hilar lymphadenopathy, and fissural nodules if present in a patient with uveitis, could help differentiate ocular sarcoidosis from ocular tuberculosis in a high TB endemic population.", "gemini": "MỤC ĐÍCH Nhằm tìm ra các dấu hiệu để phân biệt bệnh sarcoidosis ở mắt với bệnh lao ở mắt trong quần thể có tỷ lệ mắc bệnh lao cao. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu đa trung tâm hồi cứu liên quan đến bảy trung tâm chăm sóc mắt bậc ba ở Ấn Độ từ tháng 7 năm 2016 đến tháng 12 năm 2016. Dữ liệu nhân khẩu học, lâm sàng và xét nghiệm được lấy từ cơ sở dữ liệu của từng bệnh viện. Nhóm A bao gồm những trường hợp được sinh thiết chứng minh là bệnh sarcoidosis ở mắt trong khi nhóm B bao gồm bệnh lao ở mắt. KẾT QUẢ Dữ liệu từ 2726 bệnh nhân viêm màng bồ đào liên tiếp đã được lấy từ các cơ sở dữ liệu. Nhóm A có 61 trường hợp trong khi nhóm B có 307 trường hợp. Độ tuổi trung bình ở nhóm A là 43 ± 16,55 tuổi trong khi ở nhóm B là 38 ± 13,13 tuổi. Nữ giới phổ biến hơn ở nhóm A. Viêm màng bồ đào với xét nghiệm Schirmer thấp (OR-30, CI-95%), viêm mạch võng mạc nến (OR-8,69, CI-95%), hạch trung thất (OR-15,23, CI-95%), các nốt nứt (OR-39,11, CI-95%) có tỷ lệ mắc bệnh sarcoidosis ở mắt cao hơn. KẾT LUẬN Sự hiện diện của khô mắt, viêm mạch võng mạc nến, hạch trung thất và các nốt nứt nếu có ở bệnh nhân viêm màng bồ đào có thể giúp phân biệt bệnh sarcoidosis ở mắt với bệnh lao ở mắt trong quần thể có tỷ lệ mắc bệnh lao cao."} +{"idx": 1870402, "en": "INTRODUCTION The treatment of low-grade upper tract urothelial carcinomas (UTUCs) after either surgery, or nephron-sparing techniques remains an unmet need in Genitourinary (GU) Oncology. UGN-101 is a novel drug in development for the treatment of UTUCs; it is composed of a sustained-release hydrogel polymer-based formulation containing the antitumor antibiotic mitomycin-C (MM-C); cold UGN-101 is liquid, but at body temperature, it becomes a gel, and thus, when administered through a ureteral catheter, it sticks to the upper tract urothelium, slowly releasing MM-C. AREAS COVERED Here, the authors review the preclinical rationale for the development of UGN-101, as well as presently available clinical results for the treatment of low-grade UTUCs. EXPERT OPINION The positive results of the recently completed OLYMPUS trial suggest the feasibility, activity (59% of complete responses, with just 6 of these complete responders on follow-up who recurred), and safety (68% of patients experiencing mild to moderate urinary adverse events) of UGN-101 instillations into the upper urinary tract. Our expectations are that UGN-101 will soon become a standard of treatment for low-grade UTUC at risk of relapse after either surgery, or nephron-sparing techniques.", "gemini": "GIỚI THIỆU Việc điều trị ung thư biểu mô đường tiết niệu trên (UTUC) cấp độ thấp sau phẫu thuật hoặc các kỹ thuật bảo tồn nephron vẫn là nhu cầu chưa được đáp ứng trong Ung thư tiết niệu sinh dục (GU). UGN-101 là một loại thuốc mới đang được phát triển để điều trị UTUC; nó bao gồm một công thức dạng gel dựa trên polyme giải phóng chậm chứa kháng sinh chống khối u mitomycin-C (MM-C); UGN-101 lạnh ở dạng lỏng, nhưng ở nhiệt độ cơ thể, nó trở thành dạng gel và do đó, khi đưa qua ống thông niệu quản, nó sẽ bám vào biểu mô đường tiết niệu trên, từ từ giải phóng MM-C. CÁC LĨNH VỰC ĐƯỢC BAO PHỦ Ở đây, các tác giả xem xét cơ sở tiền lâm sàng cho sự phát triển của UGN-101, cũng như các kết quả lâm sàng hiện có để điều trị UTUC cấp độ thấp. Ý KIẾN CỦA CHUYÊN GIA Các kết quả khả quan của thử nghiệm OLYMPUS mới hoàn thành cho thấy tính khả thi, hoạt động (59% đáp ứng hoàn toàn, chỉ có 6 người đáp ứng hoàn toàn khi theo dõi tái phát) và tính an toàn (68% bệnh nhân gặp các tác dụng phụ về tiết niệu nhẹ đến trung bình) của việc nhỏ UGN-101 vào đường tiết niệu trên. Chúng tôi hy vọng rằng UGN-101 sẽ sớm trở thành tiêu chuẩn điều trị UTUC cấp độ thấp có nguy cơ tái phát sau phẫu thuật hoặc các kỹ thuật bảo tồn nephron."} +{"idx": 1870403, "en": "Ocular lesions in patients with human immunodeficiency virus (HIV) are commonly due to underlying opportunistic infections. With highly active antiretroviral therapy (HAART), infectious lesions have reduced and noninfectious ocular manifestations including drug-related side effects have been noted. While retinal toxicity has been noted with few other HAART drugs, there are not many on the same with Efavirenz usage. We report a series of five patients with possible efavirenz-related retinal toxicity, visual function abnormalities, and its management. Efavirenz was replaced with alternate anti-retroviral drug. Reversal of ocular side effects were noted subjectively in the form of symptom amelioration of the patients. Objectively, it could be documented with electroretinogram changes and other visual function tests reverting back to normal after change in HAART regime. Early identification of this uncommon side effect in select patients can prevent irreversible vision loss due to efavirenz-associated retinal toxicity.", "gemini": "Các tổn thương mắt ở những bệnh nhân nhiễm virus gây suy giảm miễn dịch ở người (HIV) thường là do các bệnh nhiễm trùng cơ hội tiềm ẩn. Với liệu pháp kháng retrovirus hoạt động cao (HAART), các tổn thương do nhiễm trùng đã giảm và các biểu hiện trên mắt không do nhiễm trùng bao gồm cả tác dụng phụ liên quan đến thuốc đã được ghi nhận. Trong khi độc tính trên võng mạc đã được ghi nhận với một số ít các loại thuốc HAART khác, thì không có nhiều loại thuốc tương tự khi sử dụng Efavirenz. Chúng tôi báo cáo một loạt năm bệnh nhân có thể bị độc tính trên võng mạc liên quan đến efavirenz, bất thường về chức năng thị giác và cách xử trí. Efavirenz đã được thay thế bằng thuốc kháng retrovirus khác. Tác dụng phụ trên mắt đã được đảo ngược một cách chủ quan dưới dạng cải thiện triệu chứng của bệnh nhân. Về mặt khách quan, có thể ghi nhận được sự thay đổi của điện võng mạc đồ và các xét nghiệm chức năng thị giác khác trở lại bình thường sau khi thay đổi chế độ HAART. Việc phát hiện sớm tác dụng phụ không phổ biến này ở những bệnh nhân được chọn có thể ngăn ngừa mất thị lực không hồi phục do độc tính trên võng mạc liên quan đến efavirenz."} +{"idx": 1870404, "en": "The recently identified novel coronavirus (CoV), the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), causes the coronavirus disease 2019 (COVID-19). While published data about other highly infectious human COVs [that is, the severe acute respiratory syndrome coronavirus (SARS-CoV) and the Middle East respiratory syndrome coronavirus (MERS-CoV)] provide helpful information about the infectivity of SARS-CoV-2, there is limited understanding surrounding knowledge of ocular manifestation of the virus. This paper reviews published data which reveal the presence of SARS-CoV-2 RNA in tears and conjunctival scrappings of some COVID-19 patients by real-time-polymerase chain reaction assay, although the detection rate is low compared to samples from respiratory sites. Nevertheless, the ocular complications from SARS-CoV-2 infection are uncommon. The evidence partly supports the eye as a portal of entry for SARS-CoV-2 to infect respiratory cells or viral shedding from respiratory cells via the nasolacrimal duct unto the ocular surface. The possibility of ocular secretions as source for SARS-CoV-2 to spread externally has substantial public health implications.", "gemini": "Loại coronavirus (CoV) mới được xác định gần đây, virus corona gây hội chứng hô hấp cấp tính nặng-2 (SARS-CoV-2), gây ra bệnh do virus corona 2019 (COVID-19). Trong khi dữ liệu được công bố về các loại COV ở người có khả năng lây nhiễm cao khác [nghĩa là virus corona gây hội chứng hô hấp cấp tính nặng (SARS-CoV) và virus corona gây hội chứng hô hấp Trung Đông (MERS-CoV)] cung cấp thông tin hữu ích về khả năng lây nhiễm của SARS-CoV-2, thì vẫn còn hạn chế hiểu biết xung quanh kiến thức về biểu hiện của vi-rút trên mắt. Bài báo này xem xét các dữ liệu đã công bố cho thấy sự hiện diện của RNA của SARS-CoV-2 trong nước mắt và các mảnh ghép kết mạc của một số bệnh nhân COVID-19 bằng xét nghiệm phản ứng chuỗi polymerase thời gian thực, mặc dù tỷ lệ phát hiện thấp hơn so với các mẫu từ đường hô hấp. Tuy nhiên, các biến chứng về mắt do nhiễm SARS-CoV-2 không phổ biến. Bằng chứng một phần hỗ trợ mắt là một cửa ngõ để SARS-CoV-2 xâm nhập vào các tế bào hô hấp hoặc vi-rút thải ra từ các tế bào hô hấp qua ống lệ mũi xuống bề mặt mắt. Khả năng tiết dịch ở mắt là nguồn lây lan SARS-CoV-2 ra bên ngoài có ý nghĩa đáng kể đối với sức khỏe cộng đồng."} +{"idx": 1870405, "en": "BACKGROUND Our hypothesis was that higher average club head speed is correlated with lower back injuries in professional golfers. METHODS This was a retrospective case control study of male professional golfers who suffered lower back injuries while playing golf. The injured group was composed of 14 Professional Golfers' Association (PGA) golfers who withdrew from a PGA tour event due to a back injury during the years 2017-2019. The case-control matching procedure was used to randomly match cases and controls with a 2:1 allocation ratio, respectively, based on age. Variables were chosen based on currently proposed risk factors. Data was statistically analyzed using SPSS 25. RESULTS There were 14 PGA golfers who suffered lower back injuries during the years 2017-2019 who were included in this study. There was no significant difference in age, height, weight or BMI between the injured and control group. The injured group had a higher mean club head speed than the control group (P < 0.01). CONCLUSION This study found that average club head speed was significantly higher in PGA golfers who suffered back injuries while golfing during a two-year period (2017-2019) when compared with age-matched controls.", "gemini": "BỐI CẢNH Giả thuyết của chúng tôi là tốc độ đầu gậy trung bình cao hơn có tương quan với chấn thương lưng dưới ở các vận động viên chơi gôn chuyên nghiệp. PHƯƠNG PHÁP Đây là một nghiên cứu đối chứng có hồi cứu đối với các vận động viên chơi gôn chuyên nghiệp nam bị chấn thương lưng dưới trong khi chơi gôn. Nhóm bị thương bao gồm 14 vận động viên chơi gôn của Hiệp hội golf chuyên nghiệp (PGA) đã rút khỏi một sự kiện của PGA do chấn thương lưng trong những năm 2017-2019. Quy trình ghép nối đối chứng được sử dụng để ghép ngẫu nhiên các trường hợp và đối chứng với tỷ lệ phân bổ 2:1, tương ứng, dựa trên độ tuổi. Các biến được chọn dựa trên các yếu tố rủi ro được đề xuất hiện tại. Dữ liệu được phân tích thống kê bằng SPSS 25. KẾT QUẢ Có 14 vận động viên chơi gôn PGA bị chấn thương lưng dưới trong những năm 2017-2019 được đưa vào nghiên cứu này. Không có sự khác biệt đáng kể về tuổi, chiều cao, cân nặng hoặc BMI giữa nhóm bị thương và nhóm đối chứng. Nhóm bị thương có tốc độ đầu gậy trung bình cao hơn nhóm đối chứng (P < 0,01). KẾT LUẬN Nghiên cứu này phát hiện ra rằng tốc độ đầu gậy trung bình cao hơn đáng kể ở các vận động viên chơi gôn PGA bị chấn thương lưng khi chơi gôn trong giai đoạn hai năm (2017-2019) khi so sánh với nhóm đối chứng được ghép theo độ tuổi."} +{"idx": 1870406, "en": "Prognostic role of chromosomal translocations (CT) in myelodysplasia (MDS) was retrospectively analyzed in 77 patients from GROM-L registry. Forty (51.9%) balanced, 28 (36.4%) unbalanced and 9 (11.7%) concomitant balanced and unbalanced CT were identified. Five-year overall survival (OS) of the entire cohort was 34.5% (CI 95% 22.5-46.5). Five-year OS of patients with unbalanced CT was significantly shorter than that of patients carrying balanced CT [22.3% (CI 95% 4.0-40.6) vs 44.0% (CI 95% 26.7-61.3) (p = 0.042)]. Five-year OS of patients with CT included in complex karyotype (CK) was significantly shorter than that of patients with isolated CT or CT with another abnormality [5.5% (CI 95% 0-15.7) vs 42.9% (CI 95% 21.3-64.5) and vs 4% (CI 95% 31.6-79.2) (p < 0.001)]. Presence of CT in MDS characterizes a more aggressive outcome only when associated with CK.", "gemini": "Vai trò tiên lượng của các chuyển đoạn nhiễm sắc thể (CT) trong loạn sản tủy (MDS) đã được phân tích hồi cứu ở 77 bệnh nhân từ sổ đăng ký GROM-L. Bốn mươi (51,9%) cân bằng, 28 (36,4%) không cân bằng và 9 (11,7%) đồng thời cân bằng và không cân bằng CT được xác định. Tỷ lệ sống chung sau 5 năm (OS) của toàn bộ nhóm là 34,5% (CI 95% 22,5-46,5). Tỷ lệ sống sau 5 năm của những bệnh nhân có CT không cân bằng ngắn hơn đáng kể so với những bệnh nhân mang CT cân bằng [22,3% (CI 95% 4,0-40,6) so với 44,0% (CI 95% 26,7-61,3) (p = 0,042)]. Tỷ lệ sống sau 5 năm của những bệnh nhân có CT nằm trong kiểu nhiễm sắc thể phức tạp (CK) ngắn hơn đáng kể so với những bệnh nhân có CT đơn lập hoặc CT với bất thường khác [5,5% (CI 95% 0-15,7) so với 42,9% (CI 95% 21,3-64,5) và so với 4% (CI 95% 31,6-79,2) (p < 0,001)]. Sự có mặt của CT trong MDS chỉ đặc trưng cho kết cục hung hăng hơn khi liên quan đến CK."} +{"idx": 1870407, "en": "Spinal cord infarction (SCI) occurs rarely and is characterized by abrupt onset of neck or back pain and neurologic deterioration. Fibrocartilaginous embolism (FCE) of the spinal cord is a rare but possible cause of acutely progressive spinal cord symptoms. Here, we report the case of an older woman who developed acute paraplegia with SCI on the 10th day after thoracic spine surgery. Although definitive FCE diagnosis can be confirmed only histologically, the characteristic clinical and radiological features were highly suggestive of FCE. Furthermore, 40 clinically suspected cases of FCE are reviewed.", "gemini": "Nhồi máu tủy sống (SCI) xảy ra rất hiếm và được đặc trưng bởi tình trạng đau cổ hoặc lưng đột ngột và suy giảm thần kinh. Tắc mạch sợi sụn (FCE) của tủy sống là một nguyên nhân hiếm nhưng có thể gây ra các triệu chứng tủy sống tiến triển cấp tính. Ở đây, chúng tôi báo cáo trường hợp một phụ nữ lớn tuổi bị liệt nửa người cấp tính với SCI vào ngày thứ 10 sau phẫu thuật cột sống ngực. Mặc dù chẩn đoán FCE xác định chỉ có thể được xác nhận bằng mô học, nhưng các đặc điểm lâm sàng và hình ảnh đặc trưng rất gợi ý đến FCE. Hơn nữa, 40 trường hợp nghi ngờ lâm sàng về FCE được xem xét lại."} +{"idx": 1870408, "en": "Methadone tablets were approved for use in Japan in March 2013. The metabolism of methadone is complex and executed mainly by the cytochromes, CYP3A4 and CYP2B6. The aim of this study was to evaluate the pharmacokinetics of methadone upon oral administration in Japanese patients who experienced cancer-related pain. The concentration of the drug in blood samples was measured in 25 patients undergoing methadone therapy, and the factors leading to variations were investigated. A population pharmacokinetic analysis was evaluated using the Phoenix® NLMETM software. Based on this, the ALBI (albumin-bilirubin) score was identified as a significant factor that could be used to assess variations in the serum concentration of methadone, which was then incorporated into the following final model formula: clearance (L/h) = 5.38 × (ALBI score/-2.139)1.88. The results of these pharmacokinetic parameters suggested that, in clinical use, the dose of methadone should be reduced if liver function declined in patients with cancer-related pain.", "gemini": "Viên methadone đã được chấp thuận sử dụng tại Nhật Bản vào tháng 3 năm 2013. Quá trình chuyển hóa methadone rất phức tạp và chủ yếu được thực hiện bởi cytochromes, CYP3A4 và CYP2B6. Mục đích của nghiên cứu này là đánh giá dược động học của methadone khi dùng đường uống ở những bệnh nhân Nhật Bản bị đau liên quan đến ung thư. Nồng độ thuốc trong mẫu máu được đo ở 25 bệnh nhân đang điều trị bằng methadone và các yếu tố dẫn đến sự biến đổi đã được nghiên cứu. Phân tích dược động học quần thể đã được đánh giá bằng phần mềm Phoenix® NLMETM. Dựa trên cơ sở này, điểm ALBI (albumin-bilirubin) được xác định là một yếu tố quan trọng có thể được sử dụng để đánh giá sự biến đổi nồng độ methadone trong huyết thanh, sau đó được đưa vào công thức mô hình cuối cùng sau: độ thanh thải (L/giờ) = 5,38 × (điểm ALBI/-2,139)1,88. Kết quả của các thông số dược động học này cho thấy, trong sử dụng lâm sàng, liều methadone nên được giảm nếu chức năng gan suy giảm ở những bệnh nhân bị đau liên quan đến ung thư."} +{"idx": 1870409, "en": "Hyperthermic therapy is defined as increasing the temperature of tumor tissues to 40-43 °C that has been effective approach for destroying malignant cells in the field of cancer therapy. Recent line of research has applied different approaches along with hyperthermic treatment to obtain high efficiency and little side effects. Magnetic nanoparticle-based hyperthermia has demonstrated an improved functionality in targeting malignant cells and implement their therapeutic role by heating the tumor cells. Here in this review article, we clarify the diverse aspects of magnetic nanoparticles in the treatment of cancer.", "gemini": "Liệu pháp tăng nhiệt được định nghĩa là tăng nhiệt độ của mô khối u lên 40-43 °C, đây là phương pháp hiệu quả để tiêu diệt các tế bào ác tính trong lĩnh vực điều trị ung thư. Các nghiên cứu gần đây đã áp dụng các phương pháp khác nhau cùng với phương pháp điều trị tăng nhiệt để đạt được hiệu quả cao và ít tác dụng phụ. Phương pháp tăng nhiệt dựa trên hạt nano từ tính đã chứng minh được chức năng cải tiến trong việc nhắm mục tiêu các tế bào ác tính và thực hiện vai trò điều trị của chúng bằng cách làm nóng các tế bào khối u. Trong bài tổng quan này, chúng tôi làm rõ các khía cạnh khác nhau của hạt nano từ tính trong điều trị ung thư."} +{"idx": 1870410, "en": "Our understanding of human memory has gained greatly from the study of individuals with impaired memory but rather less from outstandingly high levels of memory performance. Exceptions include the case of London taxi drivers whose extensive route learning results in modification of their hippocampus. Our study involves a group whose extensive verbal learning potentially provides a similar natural experiment. The Muslim faith encourages followers to memorise the whole of the Qur'an, some 77,449 words in its classic Arabic form. Successful memorisers are known as \"Hafiz\". We tested 10 Hafiz, 12 background-matched Muslim controls and 10 non-Muslim participants, on their detailed knowledge of the Qur'an and on their performance on standard measures of verbal and visuospatial learning. We found no differences between the three groups in their capacity to memorise verbal or visuospatial material and hence no evidence of generalisation of learning capacity in the Hafiz group. More surprisingly, however, half of the Hafiz group did not understand Arabic but were equivalent in Qur'anic memory to those who did. Given the importance that meaning is typically assumed to play in long-term memory, this was unexpected. We discuss the practical and theoretical implications of these results for verbal memory and long-term learning.", "gemini": "Sự hiểu biết của chúng ta về trí nhớ của con người đã đạt được rất nhiều từ việc nghiên cứu những cá nhân bị suy giảm trí nhớ nhưng ít hơn nhiều từ những mức hiệu suất trí nhớ cao nổi bật. Các trường hợp ngoại lệ bao gồm trường hợp của những người lái xe taxi ở London, những người học tuyến đường rộng rãi dẫn đến sự thay đổi hồi hải mã của họ. Nghiên cứu của chúng tôi liên quan đến một nhóm mà việc học tập bằng lời nói rộng rãi của họ có khả năng cung cấp một thí nghiệm tự nhiên tương tự. Đạo Hồi khuyến khích những người theo đạo ghi nhớ toàn bộ kinh Qur'an, khoảng 77.449 từ trong dạng tiếng Ả Rập cổ điển của nó. Những người ghi nhớ thành công được gọi là \"Hafiz\". Chúng tôi đã kiểm tra 10 Hafiz, 12 đối chứng là người Hồi giáo phù hợp với lý lịch và 10 người không theo đạo Hồi về kiến thức chi tiết của họ về kinh Qur'an và về thành tích của họ trên các biện pháp tiêu chuẩn về học tập bằng lời nói và thị giác không gian. Chúng tôi không tìm thấy sự khác biệt giữa ba nhóm về khả năng ghi nhớ tài liệu bằng lời nói hoặc thị giác không gian và do đó không có bằng chứng về sự khái quát hóa khả năng học tập trong nhóm Hafiz. Tuy nhiên, đáng ngạc nhiên hơn là một nửa nhóm Hafiz không hiểu tiếng Ả Rập nhưng có trí nhớ kinh Qur'an tương đương với những người hiểu. Xét đến tầm quan trọng mà ý nghĩa thường được cho là đóng vai trò trong trí nhớ dài hạn, thì điều này là không ngờ tới. Chúng tôi thảo luận về những ý nghĩa thực tiễn và lý thuyết của những kết quả này đối với trí nhớ bằng lời nói và học tập dài hạn."} +{"idx": 1870411, "en": "Autobiographical memory (AM) represents the ability to remember personal experiences. There are several laboratory or neuropsychological tasks to assess different aspects of memory function. However, there has been little research on self-reported AM ability. The Survey of Autobiographical Memory (SAM) is a self-report questionnaire, developed to assess individual differences in AM. Evidence for the factor structure and network properties of the SAM is still needed. Here, using an integral approach, we validated the SAM using a Multiple Correspondence Analysis (Study 1) as in the original study, a Confirmatory Factor Analysis (Study 2), and a Network Analysis (Study 3) using two large independent samples (total n = 2896). We first replicated the original findings, then confirmed the existence of 4 factors and found that the Episodic and Semantic categories were the most central ones. Finally, we found between \"groups\" differences for Gender and Anxiety. Overall, three different methods revealed a robust profile of the SAM.", "gemini": "Trí nhớ tự truyện (AM) là khả năng ghi nhớ những trải nghiệm cá nhân. Có một số nhiệm vụ trong phòng thí nghiệm hoặc thần kinh học để đánh giá các khía cạnh khác nhau của chức năng trí nhớ. Tuy nhiên, có rất ít nghiên cứu về khả năng AM tự báo cáo. Khảo sát về trí nhớ tự truyện (SAM) là một bảng câu hỏi tự báo cáo, được phát triển để đánh giá sự khác biệt giữa các cá nhân trong AM. Bằng chứng về cấu trúc nhân tố và các đặc tính mạng lưới của SAM vẫn còn cần thiết. Ở đây, sử dụng một phương pháp tiếp cận tích hợp, chúng tôi đã xác nhận SAM bằng cách sử dụng Phân tích tương ứng đa (Nghiên cứu 1) như trong nghiên cứu ban đầu, Phân tích nhân tố xác nhận (Nghiên cứu 2) và Phân tích mạng (Nghiên cứu 3) sử dụng hai mẫu độc lập lớn (tổng số n = 2896). Đầu tiên, chúng tôi đã sao chép các phát hiện ban đầu, sau đó xác nhận sự tồn tại của 4 yếu tố và thấy rằng các loại Tập phim và Ngữ nghĩa là những loại trung tâm nhất. Cuối cùng, chúng tôi tìm thấy sự khác biệt giữa \"các nhóm\" về Giới tính và Lo lắng. Nhìn chung, ba phương pháp khác nhau đã tiết lộ một hồ sơ mạnh mẽ của SAM."} +{"idx": 1870412, "en": "Undergraduate attrition from science, technology, engineering, and mathematics is well documented and generally intensifies during intermediate years of college. Many contributing factors exist; however, a mismatch between timing of certain pedagogical approaches, such as case-based learning, and the level of students' cognitive abilities plays a crucial role. Using cognitive load theory as a foundation, we examined relationships between case-based learning versus a traditional lecture and learning gains of undergraduates within an intermediate physiology course. We hypothesized instruction via a case study would provide greater learning benefits over a traditional lecture, with gains possibly tempered by student characteristics like academic preparation, as measured by ACT scores, and academic age, as measured by credit hours completed. Results were surprising. Case-based learning did not guarantee improved learning gains compared with a traditional lecture for all equally. Students with lower ACT scores or fewer credit hours completed had lower learning gains with a case study compared with a traditional lecture. As suggested by cognitive load theory, the amount of extraneous load potentially presented by case-based learning might overwhelm the cognitive abilities of inexperienced students.", "gemini": "Tỷ lệ bỏ học đại học ở các ngành khoa học, công nghệ, kỹ thuật và toán học đã được ghi nhận rõ ràng và thường tăng mạnh trong những năm học trung cấp. Có nhiều yếu tố góp phần; tuy nhiên, sự không phù hợp giữa thời điểm của một số phương pháp sư phạm nhất định, chẳng hạn như học theo trường hợp, và trình độ năng lực nhận thức của sinh viên đóng vai trò rất quan trọng. Sử dụng lý thuyết tải nhận thức làm nền tảng, chúng tôi đã xem xét mối quan hệ giữa học theo trường hợp so với bài giảng truyền thống và lợi ích học tập của sinh viên đại học trong một khóa sinh lý học trung cấp. Chúng tôi đưa ra giả thuyết rằng hướng dẫn thông qua một nghiên cứu trường hợp sẽ mang lại lợi ích học tập lớn hơn so với bài giảng truyền thống, với những lợi ích có thể được điều chỉnh bởi các đặc điểm của sinh viên như sự chuẩn bị học tập, được đo bằng điểm ACT, và tuổi học tập, được đo bằng số tín chỉ đã hoàn thành. Kết quả thật đáng ngạc nhiên. Học theo trường hợp không đảm bảo cải thiện kết quả học tập so với bài giảng truyền thống đối với tất cả mọi người. Học sinh có điểm ACT thấp hơn hoặc số tín chỉ hoàn thành ít hơn có kết quả học tập thấp hơn với nghiên cứu trường hợp so với bài giảng truyền thống. Theo như lý thuyết tải nhận thức, lượng tải ngoài tiềm ẩn có thể được đưa ra bởi học tập theo trường hợp có thể làm choáng ngợp khả năng nhận thức của những học sinh thiếu kinh nghiệm."} +{"idx": 1870413, "en": "Two foundational concepts in biology education are 1) offspring are not identical to their parents, and 2) organisms undergo changes throughout their lives. These concepts are included in both international and U.S. curricular standards. Research in psychology has shown that children often have difficulty understanding these concepts, as they are inconsistent with their intuitive theories of the biological world. Additionally, prior research suggests that diagrams are commonly used in instruction and that their features influence student learning. Given this prior work, we explored the characteristics of life cycle diagrams and discuss possible implications for student learning. We examined 75 life cycle diagrams from books, including five biology or general science textbooks and 25 specialized trade books focusing on biology for children. We also examined 633 life cycle diagrams from a publicly available online database of science diagrams. Most diagrams failed to show any within-species variability. Additionally, many diagrams had perceptually rich backgrounds, which prior research suggests might hinder learning. We discuss how the design characteristics of diagrams may reinforce students' intuitive theories of biology, which might make it difficult for students to understand key biological concepts in the future.", "gemini": "Hai khái niệm cơ bản trong giáo dục sinh học là 1) con cái không giống hệt cha mẹ của chúng và 2) các sinh vật trải qua những thay đổi trong suốt cuộc đời. Các khái niệm này được đưa vào cả các tiêu chuẩn giáo dục quốc tế và Hoa Kỳ. Nghiên cứu về tâm lý học đã chỉ ra rằng trẻ em thường gặp khó khăn trong việc hiểu các khái niệm này, vì chúng không phù hợp với các lý thuyết trực quan của chúng về thế giới sinh học. Ngoài ra, các nghiên cứu trước đây cho thấy sơ đồ thường được sử dụng trong hướng dẫn và các đặc điểm của chúng ảnh hưởng đến việc học của học sinh. Với công trình trước đó, chúng tôi đã khám phá các đặc điểm của sơ đồ vòng đời và thảo luận về những tác động có thể có đối với việc học của học sinh. Chúng tôi đã xem xét 75 sơ đồ vòng đời từ sách, bao gồm năm sách giáo khoa sinh học hoặc khoa học tổng quát và 25 sách chuyên ngành tập trung vào sinh học dành cho trẻ em. Chúng tôi cũng đã xem xét 633 sơ đồ vòng đời từ cơ sở dữ liệu trực tuyến về sơ đồ khoa học có sẵn công khai. Hầu hết các sơ đồ đều không cho thấy bất kỳ sự thay đổi nào trong loài. Ngoài ra, nhiều sơ đồ có nền phong phú về mặt nhận thức, mà các nghiên cứu trước đây cho thấy có thể cản trở việc học. Chúng tôi thảo luận về cách các đặc điểm thiết kế của sơ đồ có thể củng cố các lý thuyết trực quan của học sinh về sinh học, điều này có thể khiến học sinh khó hiểu các khái niệm sinh học chính trong tương lai."} +{"idx": 1870414, "en": "We previously reported that students' concept-building approaches, identified a priori using a cognitive psychology laboratory task, extend to learning complex science, technology, engineering, and mathematics topics. This prior study examined student performance in both general and organic chemistry at a select research institution, after accounting for preparation. We found that abstraction learners (defined cognitively as learning the theory underlying related examples) performed higher on course exams than exemplar learners (defined cognitively as learning by memorizing examples). In the present paper, we further examined this initial finding by studying a general chemistry course using a different pedagogical approach (process-oriented guided-inquiry learning) at an institution focused on health science majors, and then extended our studies via think-aloud interviews to probe the effect concept-building approaches have on problem-solving behaviors of average exam performance students. From interviews with students in the average-achieving group, using problems at three transfer levels, we found that: 1) abstraction learners outperformed exemplar learners at all problem levels; 2) abstraction learners relied on understanding and exemplar learners dominantly relied on an algorithm without understanding at all problem levels; and 3) both concept-building-approach students had weaknesses in their metacognitive monitoring accuracy skills, specifically their postperformance confidence level in their solution accuracy.", "gemini": "Chúng tôi đã báo cáo trước đây rằng các phương pháp xây dựng khái niệm của sinh viên, được xác định trước bằng cách sử dụng một nhiệm vụ trong phòng thí nghiệm về tâm lý học nhận thức, mở rộng đến việc học các chủ đề phức tạp về khoa học, công nghệ, kỹ thuật và toán học. Nghiên cứu trước đây này đã kiểm tra thành tích của sinh viên trong cả hóa học tổng quát và hữu cơ tại một tổ chức nghiên cứu được chọn, sau khi tính đến sự chuẩn bị. Chúng tôi thấy rằng những người học trừu tượng (được định nghĩa về mặt nhận thức là học lý thuyết cơ bản liên quan đến các ví dụ) đạt điểm cao hơn trong các kỳ thi khóa học so với những người học mẫu (được định nghĩa về mặt nhận thức là học bằng cách ghi nhớ các ví dụ). Trong bài báo hiện tại, chúng tôi đã nghiên cứu thêm phát hiện ban đầu này bằng cách nghiên cứu một khóa học hóa học tổng quát sử dụng một phương pháp sư phạm khác (học hỏi theo hướng dẫn theo quy trình) tại một tổ chức tập trung vào các chuyên ngành khoa học sức khỏe, và sau đó mở rộng nghiên cứu của chúng tôi thông qua các cuộc phỏng vấn suy nghĩ thành tiếng để thăm dò hiệu ứng của các phương pháp xây dựng khái niệm đối với hành vi giải quyết vấn đề của sinh viên có kết quả thi trung bình. Từ các cuộc phỏng vấn với những sinh viên trong nhóm đạt thành tích trung bình, sử dụng các vấn đề ở ba cấp độ chuyển đổi, chúng tôi thấy rằng: 1) những người học trừu tượng vượt trội hơn những người học mẫu ở tất cả các cấp độ vấn đề; 2) những người học trừu tượng dựa vào sự hiểu biết và những người học mẫu chủ yếu dựa vào một thuật toán mà không hiểu ở tất cả các cấp độ vấn đề; và 3) cả hai sinh viên có phương pháp xây dựng khái niệm đều có điểm yếu về kỹ năng theo dõi siêu nhận thức của họ, cụ thể là mức độ tự tin sau khi thực hiện của họ về độ chính xác của giải pháp."} +{"idx": 1870415, "en": "Research in science, technology, engineering, and mathematics education supports a shift from traditional lecturing to evidence-based instruction in college courses, yet it is unknown whether particular evidence-based pedagogies are more effective than others for learning outcomes like problem solving. Research supports three distinct pedagogies: worked examples plus practice, productive failure, and guided inquiry. These approaches vary in the nature and timing of guidance, all while engaging the learner in problem solving. Educational psychologists debate their relative effectiveness, but the approaches have not been directly compared. In this study, we investigated the impact of worked examples plus practice, productive failure, and two forms of guided inquiry (unscaffolded and scaffolded guidance) on student learning of a foundational concept in biochemistry. We compared all four pedagogies for basic knowledge performance and near-transfer problem solving, and productive failure and scaffolded guidance for far-transfer problem solving. We showed that 1) the four pedagogies did not differentially impact basic knowledge performance; 2) worked examples plus practice, productive failure, and scaffolded guidance led to greater near-transfer performance compared with unscaffolded guidance; and 3) productive failure and scaffolded guidance did not differentially impact far-transfer performance. These findings offer insights for researchers and college instructors.", "gemini": "Nghiên cứu về giáo dục khoa học, công nghệ, kỹ thuật và toán học ủng hộ sự chuyển đổi từ bài giảng truyền thống sang hướng dẫn dựa trên bằng chứng trong các khóa học đại học, nhưng vẫn chưa biết liệu các phương pháp sư phạm dựa trên bằng chứng cụ thể có hiệu quả hơn các phương pháp khác đối với các kết quả học tập như giải quyết vấn đề hay không. Nghiên cứu hỗ trợ ba phương pháp sư phạm riêng biệt: ví dụ đã làm cùng với thực hành, thất bại có hiệu quả và tìm hiểu theo hướng dẫn. Các phương pháp này khác nhau về bản chất và thời gian hướng dẫn, tất cả đều trong khi thu hút người học vào việc giải quyết vấn đề. Các nhà tâm lý học giáo dục tranh luận về hiệu quả tương đối của họ, nhưng các phương pháp này chưa được so sánh trực tiếp. Trong nghiên cứu này, chúng tôi đã điều tra tác động của các ví dụ đã làm cùng với thực hành, thất bại có hiệu quả và hai hình thức tìm hiểu theo hướng dẫn (hướng dẫn không có giá đỡ và hướng dẫn có giá đỡ) đối với việc học tập của sinh viên về một khái niệm nền tảng trong hóa sinh. Chúng tôi đã so sánh cả bốn phương pháp sư phạm về hiệu suất kiến thức cơ bản và giải quyết vấn đề chuyển giao gần, và thất bại có hiệu quả và hướng dẫn có giá đỡ để giải quyết vấn đề chuyển giao xa. Chúng tôi đã chỉ ra rằng 1) bốn phương pháp sư phạm không ảnh hưởng khác biệt đến hiệu suất kiến thức cơ bản; 2) các ví dụ đã làm cùng với thực hành, thất bại có hiệu quả và hướng dẫn có giá đỡ dẫn đến hiệu suất chuyển giao gần lớn hơn so với hướng dẫn không có giá đỡ; và 3) thất bại có hiệu quả và hướng dẫn có giá đỡ không ảnh hưởng khác biệt đến hiệu suất chuyển giao xa. Những phát hiện này cung cấp thông tin chi tiết cho các nhà nghiên cứu và giảng viên đại học."} +{"idx": 1870416, "en": "The Current Insights feature is designed to introduce life science educators and researchers to current articles of interest in other social science and education journals. In this installment, I highlight three studies drawing on psychology and learning sciences to understand how to increase student motivation to engage in scientific writing, how drawing can enhance learning, and whether spacing, or distributed practice, matters in actual classes.", "gemini": "Tính năng Thông tin chi tiết hiện tại được thiết kế để giới thiệu cho các nhà giáo dục và nhà nghiên cứu khoa học sự sống về các bài báo hiện tại được quan tâm trên các tạp chí khoa học xã hội và giáo dục khác. Trong phần này, tôi nêu bật ba nghiên cứu dựa trên tâm lý học và khoa học học tập để hiểu cách tăng động lực cho sinh viên tham gia viết khoa học, cách vẽ có thể nâng cao việc học và liệu việc giãn cách hay thực hành phân tán có vấn đề gì trong các lớp học thực tế hay không."} +{"idx": 1870417, "en": "The creation and analysis of models is integral to all scientific disciplines, and modeling is considered a core competency in undergraduate biology education. There remains a gap in understanding how modeling activities may support changes in students' neural representations. The aim of this study was to evaluate the effects of simulating a model on undergraduates' behavioral accuracy and neural response patterns when reasoning about biological systems. During brief tutorials, students (n = 30) either simulated a computer model or read expert analysis of a gene regulatory system. Subsequently, students underwent functional magnetic resonance imaging while responding to system-specific questions and system-general questions about modeling concepts. Although groups showed similar behavioral accuracy, the Simulate group showed higher levels of activation than the Read group in right cuneal and postcentral regions during the system-specific task and in the posterior insula and cingulate gyrus during the system-general task. Students' behavioral accuracy during the system-specific task correlated with lateral prefrontal brain activity independent of instruction group. Findings highlight the sensitivity of neuroimaging methods for identifying changes in representations that may not be evident at the behavioral level. This work provides a foundation for research on how distinct pedagogical approaches may affect the neural networks students engage when reasoning about biological phenomena.", "gemini": "Việc tạo ra và phân tích các mô hình là một phần không thể thiếu trong tất cả các ngành khoa học và việc lập mô hình được coi là một năng lực cốt lõi trong giáo dục sinh học bậc đại học. Vẫn còn một khoảng cách trong việc hiểu cách các hoạt động lập mô hình có thể hỗ trợ những thay đổi trong các biểu diễn thần kinh của sinh viên. Mục đích của nghiên cứu này là đánh giá các tác động của việc mô phỏng một mô hình đối với độ chính xác về hành vi và các kiểu phản ứng thần kinh của sinh viên đại học khi lập luận về các hệ thống sinh học. Trong các buổi hướng dẫn ngắn, sinh viên (n = 30) hoặc mô phỏng một mô hình máy tính hoặc đọc phân tích của chuyên gia về hệ thống điều hòa gen. Sau đó, sinh viên trải qua chụp cộng hưởng từ chức năng trong khi trả lời các câu hỏi cụ thể về hệ thống và các câu hỏi chung về hệ thống về các khái niệm lập mô hình. Mặc dù các nhóm cho thấy độ chính xác về hành vi tương tự nhau, nhưng nhóm mô phỏng cho thấy mức độ hoạt hóa cao hơn so với nhóm đọc ở vùng nêm phải và vùng sau trung tâm trong nhiệm vụ cụ thể của hệ thống và ở đảo sau và hồi vành đai trong nhiệm vụ chung của hệ thống. Độ chính xác về hành vi của sinh viên trong nhiệm vụ cụ thể của hệ thống tương quan với hoạt động não ở vùng trước trán bên không phụ thuộc vào nhóm hướng dẫn. Những phát hiện này làm nổi bật tính nhạy cảm của các phương pháp chụp ảnh thần kinh trong việc xác định những thay đổi trong các biểu diễn có thể không rõ ràng ở cấp độ hành vi. Công trình này cung cấp nền tảng cho nghiên cứu về cách các phương pháp sư phạm khác biệt có thể ảnh hưởng đến các mạng lưới thần kinh mà sinh viên tham gia khi lập luận về các hiện tượng sinh học."} +{"idx": 1870418, "en": "Cognitive scientists have recommended the use of test-enhanced learning in science classrooms. Test-enhanced learning includes the testing effect, in which learners' recall of information encountered in testing exceeds that of information not tested. The influence of incentives (e.g., points received) on learners who experience the testing effect in classrooms is less understood. The objective of our study was to examine the effects of incentives in a postsecondary biology course. We administered exams in the course using a quasi-experimental design with low and high point incentives and measured student learning. Although exposure to exams predicted better learning, incentive level did not moderate this effect, an outcome that contradicted recent laboratory findings that higher incentives decreased student recall. We discuss possible explanations of the disparate outcomes as well as the implications for further research on the testing effect in postsecondary biology classrooms.", "gemini": "Các nhà khoa học nhận thức đã khuyến nghị sử dụng phương pháp học tăng cường kiểm tra trong các lớp học khoa học. Học tập tăng cường kiểm tra bao gồm hiệu ứng kiểm tra, trong đó khả năng nhớ lại thông tin của người học gặp phải trong quá trình kiểm tra vượt quá thông tin không được kiểm tra. Ảnh hưởng của các động cơ (ví dụ: điểm nhận được) đối với những người học trải nghiệm hiệu ứng kiểm tra trong lớp học vẫn chưa được hiểu rõ. Mục tiêu của nghiên cứu của chúng tôi là kiểm tra các tác động của động cơ trong một khóa học sinh học sau trung học. Chúng tôi tiến hành các kỳ thi trong khóa học bằng cách sử dụng thiết kế bán thử nghiệm với các động cơ điểm thấp và cao và đo lường kết quả học tập của sinh viên. Mặc dù việc tiếp xúc với các kỳ thi dự đoán kết quả học tập tốt hơn, nhưng mức độ động cơ không làm giảm hiệu ứng này, một kết quả mâu thuẫn với những phát hiện gần đây trong phòng thí nghiệm cho thấy động cơ cao hơn làm giảm khả năng nhớ lại của sinh viên. Chúng tôi thảo luận về các giải thích có thể có về các kết quả khác nhau cũng như các ý nghĩa đối với các nghiên cứu sâu hơn về hiệu ứng kiểm tra trong các lớp học sinh học sau trung học."} +{"idx": 1870419, "en": "To promote undergraduate education reform, teaching professional development (TPD) efforts aim to encourage instructors to adopt evidence-based practices. However, many instructors do not attend TPD. There may be many reasons for this, including low intrinsic motivation to participate in TPD. Psychologists have dealt with motivational barriers in educational contexts using psychosocial interventions, brief activities that draw on a rich history of psychological research to subtly alter key, self-reinforcing psychological processes to yield long-term intrinsic motivation and behavioral changes. Psychosocial interventions, for example, have been used to alter students' noncognitive attitudes and beliefs, such as attributions and mindset, which positively influence students' motivation and academic performance. Here, we propose that insights from research on psychosocial interventions may be leveraged to design interventions that will increase instructors' motivation to participate in TPD, thus enhancing existing pedagogical reform efforts. We discuss psychological principles and \"best practices\" underlying effective psychosocial interventions that could guide the development of interventions to increase instructors' motivation to attend TPD. We encourage new interdisciplinary research collaborations to explore the potential of these interventions, which could be a new approach to mitigating at least one barrier to undergraduate education reform.", "gemini": "Để thúc đẩy cải cách giáo dục đại học, các nỗ lực phát triển chuyên môn giảng dạy (TPD) nhằm khuyến khích giảng viên áp dụng các thực hành dựa trên bằng chứng. Tuy nhiên, nhiều giảng viên không tham gia TPD. Có thể có nhiều lý do cho điều này, bao gồm động lực nội tại thấp để tham gia TPD. Các nhà tâm lý học đã giải quyết các rào cản về động lực trong bối cảnh giáo dục bằng cách sử dụng các can thiệp tâm lý xã hội, các hoạt động ngắn dựa trên lịch sử phong phú về nghiên cứu tâm lý để thay đổi tinh tế các quá trình tâm lý tự củng cố chính để tạo ra động lực nội tại lâu dài và thay đổi hành vi. Ví dụ, các can thiệp tâm lý xã hội đã được sử dụng để thay đổi thái độ và niềm tin phi nhận thức của học sinh, chẳng hạn như sự quy kết và tư duy, có ảnh hưởng tích cực đến động lực và thành tích học tập của học sinh. Tại đây, chúng tôi đề xuất rằng những hiểu biết từ nghiên cứu về các can thiệp tâm lý xã hội có thể được tận dụng để thiết kế các can thiệp sẽ tăng động lực của giảng viên tham gia TPD, do đó tăng cường các nỗ lực cải cách sư phạm hiện có. Chúng tôi thảo luận về các nguyên tắc tâm lý và \"thực hành tốt nhất\" cơ bản cho các can thiệp tâm lý xã hội hiệu quả có thể hướng dẫn việc phát triển các can thiệp để tăng động lực của giảng viên tham gia TPD. Chúng tôi khuyến khích các hợp tác nghiên cứu liên ngành mới để khám phá tiềm năng của các can thiệp này, có thể là một cách tiếp cận mới để giảm thiểu ít nhất một rào cản đối với cải cách giáo dục đại học."} +{"idx": 1870420, "en": "Cognitive neuroscience research is typically conducted in controlled laboratory environments that hold very little resemblance to science, technology, engineering, and mathematics classrooms. Fortunately, recent advances in portable electroencephalography technology now allow researchers to collect brain data from groups of students in real-world classrooms. Even though this line of research is still new, there is growing evidence that students' engagement, memory retention, and social dynamics are reflected in the brain-to-brain synchrony between students and teachers (i.e., the similarity in their brain responses). In this Essay, I will provide an overview of this emerging line of research, discuss how this approach can facilitate new collaborations between neuroscientists and discipline-based education researchers, and propose directions for future research.", "gemini": "Nghiên cứu khoa học thần kinh nhận thức thường được tiến hành trong môi trường phòng thí nghiệm có kiểm soát, có rất ít điểm tương đồng với các lớp học khoa học, công nghệ, kỹ thuật và toán học. May mắn thay, những tiến bộ gần đây trong công nghệ điện não đồ di động hiện cho phép các nhà nghiên cứu thu thập dữ liệu não từ các nhóm học sinh trong các lớp học thực tế. Mặc dù lĩnh vực nghiên cứu này vẫn còn mới, nhưng ngày càng có nhiều bằng chứng cho thấy sự tham gia, khả năng ghi nhớ và động lực xã hội của học sinh được phản ánh trong sự đồng bộ não với não giữa học sinh và giáo viên (tức là sự tương đồng trong phản ứng não của họ). Trong Bài tiểu luận này, tôi sẽ cung cấp một cái nhìn tổng quan về lĩnh vực nghiên cứu mới nổi này, thảo luận về cách tiếp cận này có thể tạo điều kiện cho sự hợp tác mới giữa các nhà khoa học thần kinh và các nhà nghiên cứu giáo dục theo từng chuyên ngành và đề xuất các hướng nghiên cứu trong tương lai."} +{"idx": 1870421, "en": "Epistemological beliefs about science (EBAS) or beliefs about the nature of science knowledge, and how that knowledge is generated during inquiry, are an essential yet difficult to assess component of science literacy. Leveraging learning analytics to capture and analyze student practices in simulated or game-based authentic science activities is a potential avenue for assessing EBAS. Our previous work characterized inquiry practices of experts and novices engaged in simulated authentic science inquiry and suggested that practices may reflect EBAS. Here, we extend our prior qualitative work to quantitatively examine differences in practices and EBAS between non-science majors, biology majors, and biology graduates. We observed that inquiry practices of non-science majors and biology graduates were similar to the novice and expert practices, respectively, in our prior work. However, biology majors sometimes appeared to act like their undergraduate peers (e.g., performing fewer planning actions) but other times were more similar to biology graduates (e.g., performing complex investigations). We noted that cognitive constructs like metacognition were also important for understanding which practices were most likely to be reflective of EBAS. This work advances how to assess EBAS using learning analytics and raises questions regarding the development of cognitive processes like EBAS among aspiring biologists.", "gemini": "Các niềm tin về mặt nhận thức về khoa học (EBAS) hay các niềm tin về bản chất của kiến thức khoa học và cách thức kiến thức đó được tạo ra trong quá trình tìm hiểu là một thành phần thiết yếu nhưng khó đánh giá được của khoa học. Tận dụng phân tích học tập để nắm bắt và phân tích các hoạt động của học sinh trong các hoạt động khoa học xác thực mô phỏng hoặc dựa trên trò chơi là một hướng đi tiềm năng để đánh giá EBAS. Các công trình trước đây của chúng tôi đã mô tả các hoạt động tìm hiểu của các chuyên gia và người mới bắt đầu tham gia vào quá trình tìm hiểu khoa học xác thực được mô phỏng và cho rằng các hoạt động có thể phản ánh EBAS. Ở đây, chúng tôi mở rộng công trình định tính trước đây của mình để kiểm tra định lượng sự khác biệt về hoạt động và EBAS giữa những người không chuyên về khoa học, chuyên ngành sinh học và sinh viên tốt nghiệp chuyên ngành sinh học. Chúng tôi quan sát thấy rằng các hoạt động tìm hiểu của những người không chuyên về khoa học và sinh viên tốt nghiệp chuyên ngành sinh học tương ứng với các hoạt động của người mới bắt đầu và chuyên gia trong các công trình trước đây của chúng tôi. Tuy nhiên, những sinh viên chuyên ngành sinh học đôi khi có vẻ hành động giống như những người bạn cùng lứa ở bậc đại học (ví dụ: thực hiện ít hành động lập kế hoạch hơn) nhưng đôi khi lại giống với sinh viên tốt nghiệp chuyên ngành sinh học hơn (ví dụ: thực hiện các cuộc điều tra phức tạp). Chúng tôi lưu ý rằng các cấu trúc nhận thức như siêu nhận thức cũng rất quan trọng để hiểu hoạt động nào có khả năng phản ánh EBAS nhất. Công trình này thúc đẩy cách đánh giá EBAS bằng cách sử dụng phân tích học tập và đặt ra các câu hỏi liên quan đến sự phát triển của các quá trình nhận thức như EBAS trong số các nhà sinh học đầy tham vọng."} +{"idx": 1870422, "en": "Learning objectives (LOs) are statements that typically precede a study session and describe the knowledge students should obtain by the end of the session. Despite their widespread use, limited research has investigated the effect of LOs on learning. In three laboratory experiments, we examined the extent to which LOs improve retention of information. Participants in each experiment read five passages on a neuroscience topic and took a final test that measured how well they retained the information. Presenting LOs before each corresponding passage increased performance on the final test compared with not presenting LOs (experiment 1). Actively presenting LOs increased their pedagogical value: Performance on the final test was highest when participants answered multiple-choice pretest questions compared with when they read traditional LO statements or statements that included target facts (experiment 2). Interestingly, when feedback was provided on pretest responses, performance on the final test decreased, regardless of whether the pretest format was multiple choice or short answer (experiment 3). Together, these findings suggest that, compared with the passive presentation of LO statements, pretesting (especially without feedback) is a more active method that optimizes learning.", "gemini": "Mục tiêu học tập (LO) là những tuyên bố thường đi trước một buổi học và mô tả kiến thức mà học sinh nên đạt được vào cuối buổi học. Mặc dù được sử dụng rộng rãi, nhưng các nghiên cứu hạn chế đã chỉ ra tác động của LO đối với việc học. Trong ba thí nghiệm trong phòng thí nghiệm, chúng tôi đã kiểm tra mức độ mà LO cải thiện khả năng ghi nhớ thông tin. Những người tham gia trong mỗi thí nghiệm đọc năm đoạn văn về một chủ đề về khoa học thần kinh và làm một bài kiểm tra cuối cùng để đánh giá mức độ ghi nhớ thông tin của họ. Trình bày LO trước mỗi đoạn văn tương ứng làm tăng hiệu suất trong bài kiểm tra cuối cùng so với không trình bày LO (thí nghiệm 1). Trình bày LO tích cực làm tăng giá trị sư phạm của chúng: Hiệu suất trong bài kiểm tra cuối cùng cao nhất khi những người tham gia trả lời các câu hỏi trắc nghiệm so với khi họ đọc các câu lệnh LO truyền thống hoặc các câu lệnh bao gồm các sự kiện mục tiêu (thí nghiệm 2). Điều thú vị là khi có phản hồi về các câu trả lời trắc nghiệm, hiệu suất trong bài kiểm tra cuối cùng giảm, bất kể định dạng trắc nghiệm là trắc nghiệm hay trả lời ngắn (thí nghiệm 3). Nhìn chung, những phát hiện này cho thấy rằng, so với cách trình bày thụ động các câu lệnh LO, thì việc kiểm tra trước (đặc biệt là không có phản hồi) là một phương pháp chủ động hơn giúp tối ưu hóa việc học."} +{"idx": 1870423, "en": "Recent calls in biology education research (BER) have recommended that researchers leverage learning theories and methodologies from other disciplines to investigate the mechanisms by which students to develop sophisticated ideas. We suggest design-based research from the learning sciences is a compelling methodology for achieving this aim. Design-based research investigates the \"learning ecologies\" that move student thinking toward mastery. These \"learning ecologies\" are grounded in theories of learning, produce measurable changes in student learning, generate design principles that guide the development of instructional tools, and are enacted using extended, iterative teaching experiments. In this essay, we introduce readers to the key elements of design-based research, using our own research into student learning in undergraduate physiology as an example of design-based research in BER. Then, we discuss how design-based research can extend work already done in BER and foster interdisciplinary collaborations among cognitive and learning scientists, biology education researchers, and instructors. We also explore some of the challenges associated with this methodological approach.", "gemini": "Các lời kêu gọi gần đây trong nghiên cứu giáo dục sinh học (BER) đã khuyến nghị rằng các nhà nghiên cứu nên tận dụng các lý thuyết và phương pháp học tập từ các lĩnh vực khác để điều tra các cơ chế mà sinh viên sử dụng để phát triển các ý tưởng tinh vi. Chúng tôi đề xuất rằng nghiên cứu dựa trên thiết kế từ khoa học học tập là một phương pháp hấp dẫn để đạt được mục đích này. Nghiên cứu dựa trên thiết kế điều tra \"hệ sinh thái học tập\" thúc đẩy tư duy của học sinh hướng tới sự thành thạo. Các \"hệ sinh thái học tập\" này dựa trên các lý thuyết học tập, tạo ra những thay đổi có thể đo lường được trong quá trình học tập của học sinh, tạo ra các nguyên tắc thiết kế hướng dẫn sự phát triển các công cụ hướng dẫn và được ban hành bằng các thí nghiệm giảng dạy mở rộng, lặp đi lặp lại. Trong bài tiểu luận này, chúng tôi giới thiệu cho độc giả các yếu tố chính của nghiên cứu dựa trên thiết kế, sử dụng nghiên cứu của riêng chúng tôi về quá trình học tập của sinh viên trong môn sinh lý học đại học như một ví dụ về nghiên cứu dựa trên thiết kế trong BER. Sau đó, chúng tôi thảo luận về cách nghiên cứu dựa trên thiết kế có thể mở rộng công trình đã thực hiện trong BER và thúc đẩy sự hợp tác liên ngành giữa các nhà khoa học nhận thức và học tập, các nhà nghiên cứu giáo dục sinh học và các giảng viên. Chúng tôi cũng khám phá một số thách thức liên quan đến phương pháp tiếp cận có phương pháp luận này."} +{"idx": 1870424, "en": "Today's rapidly changing world calls for sustainability-minded scientists who are prepared to solve complex, interconnected problems. Service learning is a pedagogical approach that allows students to engage with the needs of the community by integrating academic work with complex civic issues. Student learning was examined during a short-term service-learning experience focused on water-quality monitoring in an urban watershed to determine whether community-engaged fieldwork in an upper-level ecology lab course enhances sustainability knowledge for future biologists. We used concept map scoring methods and reflection assessments to evaluate and understand changes in the structure and content of student knowledge as a result of the experience. Students showed increases in sustainability knowledge breadth, depth, and complexity, particularly in demonstrating biological-sociological connections. Student reflections indicated most students identified at least one community-engaged serving-learning objective as a result of this experience. These results suggest that community-engaged fieldwork can illustrate ecological and sociological sustainability concepts for students and that engagement works best when we make explicit our objective of engaging communities in the learning process. Short service-learning experiences are effective, can be quickly assessed using concept maps, and can be readily incorporated in other classrooms to enhance sustainability education.", "gemini": "Thế giới thay đổi nhanh chóng ngày nay đòi hỏi những nhà khoa học có tư duy bền vững, những người sẵn sàng giải quyết các vấn đề phức tạp, có mối liên hệ chặt chẽ. Học tập dịch vụ là một phương pháp sư phạm cho phép sinh viên tham gia vào các nhu cầu của cộng đồng bằng cách tích hợp công việc học thuật với các vấn đề dân sự phức tạp. Quá trình học tập của sinh viên đã được kiểm tra trong một trải nghiệm học tập dịch vụ ngắn hạn tập trung vào việc theo dõi chất lượng nước tại một lưu vực đô thị để xác định liệu hoạt động thực địa có sự tham gia của cộng đồng trong khóa học phòng thí nghiệm sinh thái trình độ cao có nâng cao kiến thức về tính bền vững cho các nhà sinh vật học tương lai hay không. Chúng tôi đã sử dụng các phương pháp chấm điểm bản đồ khái niệm và đánh giá phản ánh để đánh giá và hiểu được những thay đổi trong cấu trúc và nội dung kiến thức của sinh viên sau trải nghiệm này. Sinh viên cho thấy sự gia tăng về chiều rộng, chiều sâu và tính phức tạp của kiến thức về tính bền vững, đặc biệt là trong việc chứng minh các mối liên hệ sinh học-xã hội. Những phản ánh của sinh viên chỉ ra rằng hầu hết sinh viên xác định được ít nhất một mục tiêu học tập dịch vụ có sự tham gia của cộng đồng sau trải nghiệm này. Những kết quả này cho thấy rằng hoạt động thực địa có sự tham gia của cộng đồng có thể minh họa các khái niệm về tính bền vững sinh thái và xã hội học cho sinh viên và rằng sự tham gia hoạt động tốt nhất khi chúng tôi nêu rõ mục tiêu của mình là thu hút cộng đồng vào quá trình học tập. Các trải nghiệm học tập dịch vụ ngắn hạn có hiệu quả, có thể được đánh giá nhanh chóng bằng cách sử dụng bản đồ khái niệm và có thể dễ dàng kết hợp vào các lớp học khác để nâng cao giáo dục về tính bền vững."} +{"idx": 1870425, "en": "National calls to transform undergraduate classrooms highlight the increasingly interdisciplinary nature of science, technology, engineering, and mathematics (STEM). As biologists, we use principles from chemistry and physics to make sense of the natural world. One might assume that scientists, regardless of discipline, use similar principles, resources, and reasoning to explain crosscutting phenomena. However, the context of complex natural systems can profoundly impact the knowledge activated. In this study, we used the theoretical lens of framing to explore how experts from different disciplines reasoned about a crosscutting phenomenon. Using interviews conducted with faculty (n = 10) in biology, physics, and engineering, we used isomorphic tasks to explore the impact of item context features (i.e., blood or water) on how faculty framed and reasoned about fluid dynamics, a crosscutting concept. While faculty were internally consistent in their reasoning across prompts, biology experts framed fluid dynamics problems differently than experts in physics and engineering and, as a result, used different principles and resources to reach different conclusions. These results have several implications for undergraduate learners who encounter these cross-disciplinary topics in all of their STEM courses. If each curriculum expects students to develop different reasoning strategies, students may struggle to build a coherent, transferable understanding of crosscutting phenomena.", "gemini": "Các lời kêu gọi toàn quốc nhằm chuyển đổi các lớp học đại học làm nổi bật bản chất ngày càng mang tính liên ngành của khoa học, công nghệ, kỹ thuật và toán học (STEM). Là các nhà sinh vật học, chúng tôi sử dụng các nguyên tắc từ hóa học và vật lý để hiểu thế giới tự nhiên. Người ta có thể cho rằng các nhà khoa học, bất kể chuyên ngành nào, đều sử dụng các nguyên tắc, nguồn lực và lý luận tương tự để giải thích các hiện tượng cắt ngang. Tuy nhiên, bối cảnh của các hệ thống tự nhiên phức tạp có thể ảnh hưởng sâu sắc đến kiến thức được kích hoạt. Trong nghiên cứu này, chúng tôi đã sử dụng lăng kính lý thuyết về đóng khung để khám phá cách các chuyên gia từ các lĩnh vực khác nhau lý luận về một hiện tượng cắt ngang. Sử dụng các cuộc phỏng vấn được thực hiện với giảng viên (n = 10) về sinh học, vật lý và kỹ thuật, chúng tôi đã sử dụng các nhiệm vụ đẳng cấu để khám phá tác động của các đặc điểm ngữ cảnh mục (tức là máu hoặc nước) đối với cách giảng viên đóng khung và lý luận về động lực học chất lưu, một khái niệm cắt ngang. Mặc dù các giảng viên nhất quán trong lý luận của họ trên các lời nhắc, các chuyên gia sinh học đã đóng khung các vấn đề về động lực học chất lưu khác với các chuyên gia về vật lý và kỹ thuật và do đó, sử dụng các nguyên tắc và nguồn lực khác nhau để đi đến các kết luận khác nhau. Những kết quả này có một số ý nghĩa đối với những người học đại học gặp phải những chủ đề liên ngành này trong tất cả các khóa học STEM của họ. Nếu mỗi chương trình giảng dạy mong đợi sinh viên phát triển các chiến lược lý luận khác nhau, sinh viên có thể gặp khó khăn trong việc xây dựng sự hiểu biết mạch lạc, có thể chuyển giao về các hiện tượng cắt ngang."} +{"idx": 1870426, "en": "Attention is thought to be the gateway between information and learning, yet there is much we do not understand about how students pay attention in the classroom. Leveraging ideas from cognitive neuroscience and psychology, we explore a framework for understanding attention in the classroom, organized along two key dimensions: internal/external attention and on-topic/off-topic attention. This framework helps us to build new theories for why active-learning strategies are effective teaching tools and how synchronized brain activity across students in a classroom may support learning. These ideas suggest new ways of thinking about how attention functions in the classroom and how different approaches to the same active-learning strategy may vary in how effectively they direct students' attention. We hypothesize that some teaching approaches are more effective than others because they leverage natural fluctuations in students' attention. We conclude by discussing implications for teaching and opportunities for future research.", "gemini": "Sự chú ý được coi là cầu nối giữa thông tin và việc học, tuy nhiên vẫn còn nhiều điều chúng ta chưa hiểu về cách học sinh chú ý trong lớp học. Tận dụng các ý tưởng từ khoa học thần kinh nhận thức và tâm lý học, chúng tôi khám phá một khuôn khổ để hiểu sự chú ý trong lớp học, được tổ chức dọc theo hai chiều chính: sự chú ý bên trong/bên ngoài và sự chú ý theo chủ đề/ngoài chủ đề. Khung này giúp chúng tôi xây dựng các lý thuyết mới giải thích tại sao các chiến lược học tập tích cực là các công cụ giảng dạy hiệu quả và hoạt động não được đồng bộ hóa giữa các học sinh trong lớp học có thể hỗ trợ việc học như thế nào. Những ý tưởng này gợi ra những cách suy nghĩ mới về cách chú ý hoạt động trong lớp học và cách các phương pháp tiếp cận khác nhau đối với cùng một chiến lược học tập tích cực có thể khác nhau như thế nào về hiệu quả của chúng trong việc hướng sự chú ý của học sinh. Chúng tôi đưa ra giả thuyết rằng một số phương pháp giảng dạy hiệu quả hơn những phương pháp khác vì chúng tận dụng những biến động tự nhiên trong sự chú ý của học sinh. Chúng tôi kết luận bằng cách thảo luận về những hàm ý đối với việc giảng dạy và các cơ hội cho nghiên cứu trong tương lai."} +{"idx": 1870427, "en": "Psychological theories of motivation and performance are relevant to teaching and learning in the science, technology, engineering, and mathematics (STEM) disciplines. The present study applies Dweck's mindset theory of motivation to an examination of the relationship among instructor mindset, instructor motivational attitudes, and the use of effective teaching practices. Faculty members who teach undergraduate courses in STEM disciplines completed a survey designed to assess fixed versus growth mindset, mastery orientation (measures of motivation and efficacy), and teaching practices. Results supported a model consistent with Dweck's theory of motivation, whereby mastery orientation mediates the relationship between instructor mindset and teaching behaviors. It appears that this psychological theory of motivation may be helpful in understanding teaching and learning in STEM disciplines. More research using a variety of measures and teaching contexts is necessary before full applicability can be realized.", "gemini": "Các lý thuyết tâm lý về động lực và hiệu suất có liên quan đến việc giảng dạy và học tập trong các ngành khoa học, công nghệ, kỹ thuật và toán học (STEM). Nghiên cứu hiện tại áp dụng lý thuyết tư duy của Dweck về động lực để kiểm tra mối quan hệ giữa tư duy của người hướng dẫn, thái độ thúc đẩy của người hướng dẫn và việc sử dụng các phương pháp giảng dạy hiệu quả. Các thành viên giảng viên giảng dạy các khóa học đại học trong các ngành STEM đã hoàn thành một cuộc khảo sát được thiết kế để đánh giá tư duy cố định so với tư duy phát triển, định hướng thành thạo (biện pháp về động lực và hiệu quả) và các phương pháp giảng dạy. Kết quả ủng hộ một mô hình phù hợp với lý thuyết động lực của Dweck, theo đó định hướng thành thạo làm trung gian cho mối quan hệ giữa tư duy của người hướng dẫn và hành vi giảng dạy. Có vẻ như lý thuyết động lực tâm lý này có thể hữu ích trong việc hiểu về việc giảng dạy và học tập trong các ngành STEM. Cần có thêm nhiều nghiên cứu sử dụng nhiều biện pháp và bối cảnh giảng dạy khác nhau trước khi có thể nhận ra khả năng áp dụng đầy đủ."} +{"idx": 1870428, "en": "Transfer of knowledge from one context to another is one of the paramount goals of education. Educators want their students to transfer what they are learning from one topic to the next, between courses, and into the \"real world.\" However, it is also notoriously difficult to get students to successfully transfer concepts. This issue is of particular concern in biology and the life sciences, for which transfer of concepts between disciplines is especially critical to understanding. Students not only struggle to transfer concepts like energy from chemistry to biology but also struggle to transfer concepts like chromosome structures in cell division within biology courses. This paper reviews the current research and understanding of transfer from cognitive psychology. We discuss how learner abilities, taught material, and lesson characteristics affect transfer and provide best practices for biology and life sciences education.", "gemini": "Truyền tải kiến thức từ ngữ cảnh này sang ngữ cảnh khác là một trong những mục tiêu quan trọng nhất của giáo dục. Các nhà giáo dục muốn học sinh của họ truyền tải những gì chúng đang học từ chủ đề này sang chủ đề tiếp theo, giữa các khóa học và vào \"thế giới thực\". Tuy nhiên, cũng rất khó để học sinh truyền đạt thành công các khái niệm. Vấn đề này đặc biệt đáng quan tâm trong lĩnh vực sinh học và khoa học sự sống, trong đó việc truyền tải các khái niệm giữa các ngành là đặc biệt quan trọng để hiểu. Học sinh không chỉ vật lộn để truyền đạt các khái niệm như năng lượng từ hóa học sang sinh học mà còn vật lộn để truyền đạt các khái niệm như cấu trúc nhiễm sắc thể trong quá trình phân chia tế bào trong các khóa học sinh học. Bài báo này xem xét các nghiên cứu và hiểu biết hiện tại về sự truyền đạt từ tâm lý học nhận thức. Chúng tôi thảo luận về cách khả năng của người học, tài liệu được dạy và đặc điểm của bài học ảnh hưởng đến sự truyền đạt và cung cấp các phương pháp hay nhất cho giáo dục sinh học và khoa học sự sống."} +{"idx": 1870429, "en": "The testing effect is one of the strongest learning techniques documented to date. Although the effects of testing on high-level learning are promising, fewer studies on this have been done. In this classroom application of the testing effect, we aimed to 1) determine whether a testing effect exists on high-level testing; 2) determine whether higher-level testing has an effect on low-level content retention; and 3) determine whether content knowledge, cognitive skill, or additional components are responsible for this effect. Through a series of two experiments, we confirmed a testing effect on high-level items. However, improved content retention due to testing was not observed. We suggest that this high-level testing effect is due to a better ability to apply specific skills to specific content when this application process has appeared on a previous exam.", "gemini": "Hiệu ứng kiểm tra là một trong những kỹ thuật học tập mạnh mẽ nhất được ghi nhận cho đến nay. Mặc dù hiệu quả của việc kiểm tra đối với việc học ở trình độ cao rất hứa hẹn, nhưng ít có nghiên cứu nào về vấn đề này được thực hiện. Trong ứng dụng hiệu ứng kiểm tra trong lớp học này, chúng tôi đặt mục tiêu 1) xác định xem có hiệu ứng kiểm tra đối với kiểm tra trình độ cao hay không; 2) xác định xem kiểm tra ở trình độ cao hơn có ảnh hưởng đến việc ghi nhớ nội dung ở trình độ thấp hay không; và 3) xác định xem kiến thức về nội dung, kỹ năng nhận thức hay các thành phần bổ sung có chịu trách nhiệm cho hiệu ứng này hay không. Thông qua một loạt hai thí nghiệm, chúng tôi đã xác nhận hiệu ứng kiểm tra đối với các mục ở trình độ cao. Tuy nhiên, không quan sát thấy khả năng ghi nhớ nội dung được cải thiện do kiểm tra. Chúng tôi cho rằng hiệu ứng kiểm tra ở trình độ cao này là do khả năng áp dụng các kỹ năng cụ thể tốt hơn vào nội dung cụ thể khi quá trình ứng dụng này đã xuất hiện trong kỳ thi trước."} +{"idx": 1870430, "en": "Ingrown toenails Abstract. Ingrown toenails are the most common foot problems in primary care. Untreated or mistreated ingrown toenails will lead to unacceptable long suffering of the patient with negative impact on their daily life. Still there is no consensus reached for best treatment and usually the surgical options are the last solution. One reason might be the high variety in conservative und surgical treatment. But also, the high recurrence rates after surgery with up to 30 % described in literature. We think, that these high numbers might be also a sign for improper performed surgery. In this article we would like to give you a step by step pathway in a minimal invasive partial nail avulsion with partial matricectomy, which we perform since several years in our clinic. We are convinced of the cosmetical, functional and long-term results of this procedure.", "gemini": "Móng chân mọc ngược Tóm tắt. Móng chân mọc ngược là vấn đề về chân phổ biến nhất trong chăm sóc ban đầu. Móng chân mọc ngược không được điều trị hoặc điều trị không đúng cách sẽ dẫn đến tình trạng bệnh nhân đau đớn kéo dài không thể chấp nhận được, ảnh hưởng tiêu cực đến cuộc sống hàng ngày của họ. Hiện vẫn chưa có sự thống nhất về phương pháp điều trị tốt nhất và thường thì các lựa chọn phẫu thuật là giải pháp cuối cùng. Một lý do có thể là sự đa dạng cao trong điều trị bảo tồn và phẫu thuật. Nhưng cũng có tỷ lệ tái phát cao sau phẫu thuật với tỷ lệ lên đến 30% được mô tả trong tài liệu. Chúng tôi cho rằng, những con số cao này cũng có thể là dấu hiệu của việc phẫu thuật không đúng cách. Trong bài viết này, chúng tôi muốn cung cấp cho bạn một lộ trình từng bước trong một cuộc cắt bỏ một phần móng tay tối thiểu với cắt bỏ một phần ma trận, mà chúng tôi đã thực hiện trong nhiều năm tại phòng khám của mình. Chúng tôi tin tưởng vào kết quả thẩm mỹ, chức năng và lâu dài của thủ thuật này."} +{"idx": 1870431, "en": "Urologic Emergencies: Paraphimosis Abstract. Paraphimosis presents a rare but acute urological emergency whereby the foreskin becomes entrapped behind the coronary sulcus of the penis. Therapy is quick and feasible, even in an outpatient setting. In most cases compression of the preputial edema and subsequent reposition of the prepuce is sufficient. Rarely, surgical intervention in form of a dorsal incision of the constriction is required. With partial or full phimosis being the underlying condition, paraphimosis occurs predominantly in infants and toddlers. However, persistent or secondary phimosis can lead to paraphimosis in advanced age.", "gemini": "Các trường hợp cấp cứu tiết niệu: Phimosis Tóm tắt. Phimosis là một trường hợp cấp cứu tiết niệu hiếm gặp nhưng cấp tính, trong đó bao quy đầu bị mắc kẹt phía sau rãnh vành của dương vật. Liệu pháp nhanh chóng và khả thi, ngay cả trong môi trường ngoại trú. Trong hầu hết các trường hợp, việc chèn ép phù nề bao quy đầu và sau đó đặt lại bao quy đầu là đủ. Hiếm khi, cần can thiệp phẫu thuật dưới dạng rạch mặt lưng của chỗ thắt chặt. Với tình trạng hẹp bao quy đầu một phần hoặc toàn bộ là tình trạng cơ bản, phimosis chủ yếu xảy ra ở trẻ sơ sinh và trẻ mới biết đi. Tuy nhiên, hẹp bao quy đầu dai dẳng hoặc thứ phát có thể dẫn đến phimosis ở tuổi cao."} +{"idx": 1870432, "en": "Primary wound care in children Abstract. Successful wound care of infants and children is facilitated by good preparation and a calm atmosphere. There is not only the child as a patient but also the parents, with their fears and concerns. The physician has to take care of both of them. Parent and child should be informed about the therapeutic intervention appropriately. Reassuring of the child and distraction from the procedure are as important as the treatment itself (e. g. wound stitching or application of wound dressing). Topical anesthesia with LET Gel (lidocain, epinephrine, tetracaine), non-stinging methods to clean the wound (NaCl 0,9 % / Polyhexanid (Prontosan®) soaked swabs) and intranasal application of fentanyl / dormicum can be used to avoid fear and pain. Sedation is used deliberately in small children for wound care. Laceration wounds, mainly those affecting the scalp, chin or forehead can be treated in the emergency room without general anesthesia. Extensive wounds, burns and animal bites often require wound care under anesthesia in children.", "gemini": "Chăm sóc vết thương ban đầu ở trẻ em Tóm tắt. Chăm sóc vết thương thành công cho trẻ sơ sinh và trẻ em được tạo điều kiện thuận lợi bởi sự chuẩn bị tốt và bầu không khí bình tĩnh. Không chỉ có trẻ là bệnh nhân mà còn có cha mẹ, với nỗi sợ hãi và mối quan tâm của họ. Bác sĩ phải chăm sóc cả hai người. Cha mẹ và trẻ em nên được thông báo về can thiệp điều trị một cách thích hợp. Làm dịu đứa trẻ và đánh lạc hướng khỏi quy trình cũng quan trọng như chính việc điều trị (ví dụ khâu vết thương hoặc áp dụng băng vết thương). Gây tê tại chỗ bằng LET Gel (lidocain, epinephrine, tetracaine), các phương pháp không gây đau để làm sạch vết thương (NaCl 0,9% / tăm bông ngâm Polyhexanid (Prontosan®) và ứng dụng fentanyl / dormicum qua đường mũi có thể được sử dụng để tránh sợ hãi và đau đớn. An thần được sử dụng có chủ ý ở trẻ nhỏ để chăm sóc vết thương. Vết thương rách, chủ yếu là những vết thương ảnh hưởng đến da đầu, cằm hoặc trán có thể được điều trị tại phòng cấp cứu mà không cần gây mê toàn thân. Các vết thương rộng, vết bỏng và vết cắn của động vật thường cần phải chăm sóc vết thương dưới gây mê ở trẻ em."} +{"idx": 1870433, "en": "Treatment of acute injuries of the hand Abstract. Injuries to the hand are a common presentation in primary care units. When accurately assessed, many open injuries may be handled in the emergency department without referral to a hand surgery specialist. We would like to give some recommendations on how to treat the most frequent injuries like lesions to the nail and nailbed, fingertip amputation as well as burns, infections and bites. But first, we highlight the different methods of local anesthesia and discuss the use of a tourniquet or vasoconstriction with adrenalin - WALANT - instead.", "gemini": "Điều trị chấn thương cấp tính ở bàn tay Tóm tắt. Chấn thương bàn tay là một biểu hiện phổ biến ở các đơn vị chăm sóc ban đầu. Khi được đánh giá chính xác, nhiều vết thương hở có thể được xử lý tại khoa cấp cứu mà không cần chuyển đến bác sĩ phẫu thuật chuyên khoa bàn tay. Chúng tôi muốn đưa ra một số khuyến cáo về cách điều trị các chấn thương thường gặp nhất như tổn thương móng tay và nền móng, cụt ngón tay cũng như bỏng, nhiễm trùng và vết cắn. Nhưng trước tiên, chúng tôi sẽ nêu bật các phương pháp gây tê tại chỗ khác nhau và thảo luận về việc sử dụng garô hoặc co mạch bằng adrenalin - WALANT - thay thế."} +{"idx": 1870434, "en": "Treatment of Skin Abscesses in the Emergency Department Abstract. Skin abscesses are a common cause for presentation in the emergency department and are in most cases treated by incision and drainage. The diagnosis is usually based upon clinical manifestation. If there is uncertainty regarding the presence of an abscess, bedside ultrasonography is suggested to identify the presence, size and location of the abscess. Uncomplicated abscesses < 5 cm in diameter often can be treated under local or regional anesthesia with or without additional procedural sedation within the emergency department. Sufficient anesthesia and analgesia are essential to avoid undertreatment of the abscess. Certain abscess localizations or conditions require referral to a surgeon and / or operation of the abscess in the operating room. Alternative to the classic incision and drainage the minimal invasive Loop Drainage Technique may be considered. A postoperative systemic antibiotic treatment is only indicated under certain conditions.", "gemini": "Điều trị áp xe da tại khoa cấp cứu Tóm tắt. Áp xe da là một nguyên nhân phổ biến khiến bệnh nhân đến khoa cấp cứu và trong hầu hết các trường hợp được điều trị bằng cách rạch và dẫn lưu. Chẩn đoán thường dựa trên biểu hiện lâm sàng. Nếu không chắc chắn về sự hiện diện của áp xe, siêu âm tại giường được đề xuất để xác định sự hiện diện, kích thước và vị trí của áp xe. Áp xe không biến chứng <5 cm đường kính thường có thể được điều trị bằng gây tê tại chỗ hoặc vùng với hoặc không có an thần thủ thuật bổ sung trong khoa cấp cứu. Gây tê và giảm đau đầy đủ là rất cần thiết để tránh điều trị thiếu áp xe. Một số vị trí hoặc tình trạng áp xe nhất định cần phải chuyển đến bác sĩ phẫu thuật và / hoặc phẫu thuật áp xe tại phòng mổ. Thay thế cho phương pháp rạch và dẫn lưu cổ điển có thể xem xét Kỹ thuật dẫn lưu vòng xâm lấn tối thiểu. Điều trị kháng sinh toàn thân sau phẫu thuật chỉ được chỉ định trong một số điều kiện nhất định."} +{"idx": 1870435, "en": "Onset and management of perianal vein thrombosis Abstract. Acute onset of anal pain is a classic symptom of perianal vein thrombosis. Along with the typical finding of a painful swelling perianal, the diagnosis can be made without further diagnostic measures and the targeted therapy can be initiated. An acute perianal vein thrombosis can be successfully excised within the first 72 hours.", "gemini": "Khởi phát và quản lý huyết khối tĩnh mạch quanh hậu môn Tóm tắt. Đau hậu môn khởi phát cấp tính là một triệu chứng điển hình của huyết khối tĩnh mạch quanh hậu môn. Cùng với phát hiện điển hình là sưng đau quanh hậu môn, chẩn đoán có thể được đưa ra mà không cần các biện pháp chẩn đoán thêm và có thể bắt đầu điều trị có mục tiêu. Huyết khối tĩnh mạch quanh hậu môn cấp tính có thể được cắt bỏ thành công trong vòng 72 giờ đầu."} +{"idx": 1870436, "en": "Arthrocentesis in the Emergency Department Abstract. Acute joint swelling is a common presentation to the emergency department. Although routine investigations like clinical exam, labs and eventually x-ray are usually obtained, definitive diagnosis must be established since timely recognition of septic arthritis in particular is crucial. Definitive diagnosis is achieved by performing an arthrocentesis of the affected joint. While arthrocentesis of larger joints and large effusions (e. g. knee) are relatively easy to perform using the landmark-technique, smaller and less accessible joints (shoulder, elbow, hip) are more difficult to access and it is therefore recommended to use ultrasound guidance. Compared with the landmark-technique, ultrasound-guided arthrocentesis is more successful and less painful. Synovial fluid should be analyzed for cell count with differential, crystals as well as for microbiological analysis such as Gram-stain and culture. Once the diagnosis of septic arthritis has been established, irrigation of the joint should be performed by orthopedic surgery. Antibiotic therapy should be withheld until the sampling of synovial fluid has been completed. After exclusion of septic arthritis, acute arthritis due to crystal arthropathy (CPPD or gout) is treated with either glucocorticoid-infiltration of the joint or with nonsteroidal anti-inflammatory drugs. In this article, the different technical aspects of arthrocentesis are discussed, including asepsis, landmark- and ultrasound-guided access, preanalytics and interpretation of the laboratory results.", "gemini": "Chọc hút khớp ở khoa cấp cứu Tóm tắt. Sưng khớp cấp tính là một biểu hiện phổ biến ở khoa cấp cứu. Mặc dù các xét nghiệm thường quy như khám lâm sàng, xét nghiệm và cuối cùng là chụp X quang thường được thực hiện, nhưng chẩn đoán xác định phải được thiết lập vì việc phát hiện kịp thời viêm khớp nhiễm trùng nói riêng là rất quan trọng. Chẩn đoán xác định đạt được bằng cách thực hiện chọc hút khớp bị ảnh hưởng. Trong khi chọc hút các khớp lớn hơn và tràn dịch lớn (ví dụ như đầu gối) tương đối dễ thực hiện bằng kỹ thuật đánh dấu, thì các khớp nhỏ hơn và khó tiếp cận hơn (vai, khuỷu tay, hông) khó tiếp cận hơn và do đó nên sử dụng hướng dẫn siêu âm. So với kỹ thuật đánh dấu, chọc hút khớp có hướng dẫn siêu âm thành công hơn và ít đau hơn. Dịch khớp nên được phân tích để đếm tế bào có biệt hóa, tinh thể cũng như để phân tích vi sinh vật như nhuộm Gram và nuôi cấy. Khi chẩn đoán viêm khớp nhiễm trùng đã được xác định, phẫu thuật chỉnh hình nên được thực hiện để rửa sạch khớp. Nên trì hoãn điều trị bằng kháng sinh cho đến khi hoàn thành việc lấy mẫu dịch khớp. Sau khi loại trừ viêm khớp nhiễm trùng, viêm khớp cấp do bệnh lý khớp do tinh thể (CPPD hoặc bệnh gút) được điều trị bằng cách tiêm glucocorticoid vào khớp hoặc bằng thuốc chống viêm không steroid. Trong bài viết này, các khía cạnh kỹ thuật khác nhau của chọc hút khớp được thảo luận, bao gồm vô trùng, tiếp cận có hướng dẫn bằng siêu âm và đánh dấu, tiền phân tích và giải thích kết quả xét nghiệm."} +{"idx": 1870437, "en": "How to treat an epistaxis: Tips and pitfalls Abstract. Nosebleed is a common and usually self-limiting symptom. Only 6 % of those affected need medical attention. Patients often present themselves to their family doctor or in an emergency setting. The aim of this article is to convey the important points in the diagnosis and therapy of epistaxis.", "gemini": "Cách xử trí chảy máu cam: Mẹo và cạm bẫy Tóm tắt. Chảy máu cam là một triệu chứng phổ biến và thường tự giới hạn. Chỉ có 6% những người bị ảnh hưởng cần được chăm sóc y tế. Bệnh nhân thường tự đến bác sĩ gia đình hoặc đến phòng cấp cứu. Mục đích của bài viết này là truyền đạt những điểm quan trọng trong chẩn đoán và điều trị chảy máu cam."} +{"idx": 1870438, "en": "Traumatic dislocation of the shoulder - a guide for medical practicioners Abstract. Traumatic dislocation of the shoulder joint is a common injury. Young patients suffer from dislocations usually during leisure and competitive activities, the elderly are prone for injuries because of falls. We provide an overview about state-of-the-art management in first traumatic shoulder dislocations. Besides an overview for mechanisms leading to injuries, we describe first line therapy as well as techniques to reposition the shoulder. First time traumatic glenohumeral dislocations can usually be treated without surgery, we elucidate the topic regarding the need for further imaging as well as possible injuries accompanying the dislocation.", "gemini": "Trật khớp vai do chấn thương - hướng dẫn dành cho các bác sĩ hành nghề Tóm tắt. Trật khớp vai là một chấn thương phổ biến. Bệnh nhân trẻ thường bị trật khớp trong các hoạt động giải trí và thi đấu, người cao tuổi dễ bị chấn thương do ngã. Chúng tôi cung cấp một cái nhìn tổng quan về cách xử trí hiện đại đối với trật khớp vai do chấn thương lần đầu. Bên cạnh cái nhìn tổng quan về các cơ chế dẫn đến chấn thương, chúng tôi mô tả liệu pháp điều trị đầu tay cũng như các kỹ thuật để đưa vai vào đúng vị trí. Trật khớp vai lần đầu do chấn thương thường có thể được điều trị mà không cần phẫu thuật, chúng tôi làm sáng tỏ chủ đề về nhu cầu chụp ảnh thêm cũng như các chấn thương có thể đi kèm với trật khớp."} +{"idx": 1870439, "en": "Background: Alcohol-use disorders (AUD) pose a significant challenge for the United States (US) military. The US Department of Defense has strongly recommended several medications for use in the treatment of patients with diagnosed AUD. This study assessed the prescription of medications for active duty service members (ADSMs) diagnosed with AUD in the US Military Health System (MHS). Methods: Rates of prescription orders were retrospectively examined from 2010 to 2017 among ADSMs with an incident diagnosis of moderate-to-severe AUD. The rate of prescription orders was defined as the proportion of ADSMs with an ICD-9 or ICD-10 diagnosis code of alcohol dependence who received an order for acamprosate, disulfiram, naltrexone, and/or topiramate at a military treatment facility in the year following their incident diagnosis. Results: ADSMs receiving an order for at least one medication in the year following their incident AUD diagnosis increased from 8.8% in 2010 to 16.2% in 2017 (RR = 1.84, 95% CI, 1.76, 1.93). Oral naltrexone was ordered most frequently among this patient population, while injectable naltrexone, a medication option meant to ease and improve adherence, was ordered for a smaller proportion of patients. Conclusions: Most ADSMs who might benefit from prescriptions for AUD are not receiving them as part of their treatment despite strong clinical evidence and Department of Defense policy support for their use among this cohort.", "gemini": "Bối cảnh: Rối loạn sử dụng rượu (AUD) đặt ra thách thức đáng kể đối với quân đội Hoa Kỳ (Mỹ). Bộ Quốc phòng Hoa Kỳ đã khuyến cáo mạnh mẽ một số loại thuốc để sử dụng trong điều trị bệnh nhân được chẩn đoán mắc AUD. Nghiên cứu này đánh giá đơn thuốc cho các quân nhân đang phục vụ tích cực (ADSM) được chẩn đoán mắc AUD trong Hệ thống Y tế Quân đội Hoa Kỳ (MHS). Phương pháp: Tỷ lệ đơn thuốc đã được kiểm tra hồi cứu từ năm 2010 đến năm 2017 trong số các ADSM được chẩn đoán mắc AUD từ trung bình đến nặng. Tỷ lệ đơn thuốc được định nghĩa là tỷ lệ ADSM có mã chẩn đoán ICD-9 hoặc ICD-10 về chứng nghiện rượu đã nhận được đơn thuốc acamprosate, disulfiram, naltrexone và/hoặc topiramate tại cơ sở điều trị quân y trong năm sau khi chẩn đoán mắc bệnh. Kết quả: ADSM nhận được đơn thuốc cho ít nhất một loại thuốc trong năm sau khi chẩn đoán mắc AUD đã tăng từ 8,8% vào năm 2010 lên 16,2% vào năm 2017 (RR = 1,84, KTCB 95%, 1,76, 1,93). Naltrexone đường uống được kê đơn nhiều nhất trong nhóm bệnh nhân này, trong khi naltrexone tiêm, một lựa chọn thuốc nhằm giảm bớt và cải thiện khả năng tuân thủ, được kê đơn cho một tỷ lệ nhỏ bệnh nhân. Kết luận: Hầu hết các ADSM có thể được hưởng lợi từ đơn thuốc cho AUD đều không nhận được đơn thuốc này như một phần trong quá trình điều trị của họ mặc dù có bằng chứng lâm sàng mạnh mẽ và chính sách của Bộ Quốc phòng ủng hộ việc sử dụng đơn thuốc này trong nhóm đối tượng này."} +{"idx": 1870440, "en": "Colistin is a polypeptide antibiotic mainly used in porcine and poultry to treat gastrointestinal infections. It has been included by the World Health Organisation (WHO) in the list of critically important human antibiotics of high priority for antimicrobial resistance since 2017. Therefore, it is necessary to develop specific and sensitive screening methods for this molecule. Screening for colistin with immunoassays is an interesting alternative to LC-MS/MS screening methods. The performance of three commercially available ELISA kits was evaluated in poultry and porcine muscles for the detection of colistin in regards to its European maximum residue limit (MRL) (150 µg/kg). The applicability of the three ELISA kits to the detection of colistin at or below the MRL in porcine and poultry muscles was demonstrated. The detection capabilities (CCβ) of two kits were or lower than or equal to the MRL (150 µg/kg). The lowest detection capability (30 µg/kg) was achieved with the third ELISA kit. The specificity of the three kits was very satisfactory (false positive rates 0%). The three kits are very specific for the detection of colistin (colistin A and B) and polymyxin B.", "gemini": "Colistin là một loại kháng sinh polypeptide chủ yếu được sử dụng ở lợn và gia cầm để điều trị nhiễm trùng đường tiêu hóa. Tổ chức Y tế Thế giới (WHO) đã đưa colistin vào danh sách các loại kháng sinh quan trọng đối với con người có mức độ ưu tiên cao về tình trạng kháng thuốc chống vi khuẩn kể từ năm 2017. Do đó, cần phải phát triển các phương pháp sàng lọc đặc hiệu và nhạy cảm đối với phân tử này. Sàng lọc colistin bằng phương pháp miễn dịch là một giải pháp thay thế thú vị cho các phương pháp sàng lọc LC-MS/MS. Hiệu suất của ba bộ kit ELISA thương mại đã được đánh giá ở các cơ bắp của gia cầm và lợn để phát hiện colistin liên quan đến giới hạn dư lượng tối đa (MRL) của Châu Âu (150 µg/kg). Khả năng ứng dụng của ba bộ kit ELISA để phát hiện colistin ở hoặc dưới MRL trong cơ bắp của gia cầm và lợn đã được chứng minh. Khả năng phát hiện (CCβ) của hai bộ kit thấp hơn hoặc bằng MRL (150 µg/kg). Khả năng phát hiện thấp nhất (30 µg/kg) đạt được với bộ kit ELISA thứ ba. Độ đặc hiệu của ba bộ kit rất khả quan (tỷ lệ dương tính giả 0%). Cả ba bộ kit đều rất đặc hiệu trong việc phát hiện colistin (colistin A và B) và polymyxin B."} +{"idx": 1870441, "en": "Episodic memory performance declines with increasing age. It has sometimes been reported that this decline is more marked when episodic recollection is estimated by \"objective\" measures such as source memory performance than when it is estimated by \"subjective\" measures such as the \"Remember/Know\" procedure. Here, our main goal was to directly contrast recollection estimates derived from these procedures in the same samples of young and older participants (24 adults per age group, within-subjects manipulation of test procedure). Following identical study phases in which words were paired with either faces or scenes, participants' memories were assessed in separate test blocks using either Remember/Know or source memory procedures. Contrary to several prior reports, the deleterious effects of age on recollection estimates did not differ according to test type. Thus, we found no evidence that age differentially impacts subjective and objective recollection estimates. Additionally, and consistent with prior findings, effects of age on estimates of familiarity-driven recognition were small and non-significant.", "gemini": "Hiệu suất trí nhớ theo từng giai đoạn giảm dần theo tuổi tác. Đôi khi, người ta đã báo cáo rằng sự suy giảm này rõ rệt hơn khi hồi tưởng theo từng giai đoạn được ước tính bằng các biện pháp \"khách quan\" như hiệu suất trí nhớ nguồn hơn là khi nó được ước tính bằng các biện pháp \"chủ quan\" như quy trình \"Nhớ/Biết\". Ở đây, mục tiêu chính của chúng tôi là trực tiếp đối chiếu các ước tính hồi tưởng có được từ các quy trình này trong cùng một mẫu người tham gia trẻ và lớn tuổi (24 người lớn trên mỗi nhóm tuổi, thao tác trong đối tượng về quy trình kiểm tra). Sau các giai đoạn nghiên cứu giống hệt nhau trong đó các từ được ghép nối với khuôn mặt hoặc cảnh, trí nhớ của người tham gia được đánh giá trong các khối kiểm tra riêng biệt bằng cách sử dụng quy trình Nhớ/Biết hoặc quy trình trí nhớ nguồn. Trái ngược với một số báo cáo trước đây, các tác động có hại của tuổi tác đối với các ước tính hồi tưởng không khác nhau tùy theo loại kiểm tra. Do đó, chúng tôi không tìm thấy bằng chứng nào cho thấy tuổi tác ảnh hưởng khác nhau đến các ước tính hồi tưởng chủ quan và khách quan. Ngoài ra, và phù hợp với các phát hiện trước đây, các tác động của tuổi tác đối với các ước tính nhận dạng theo sự quen thuộc là nhỏ và không đáng kể."} +{"idx": 1870442, "en": "Background: Community pharmacists can play a meaningful role in identification and treatment of substance use disorders (SUD). However, inadequate disease knowledge and negative attitudes are known barriers. The relationship between knowledge, attitudes, and practice of pharmacists regarding persons with SUD has not been evaluated comprehensively in the United States. The objective of the study was to assess knowledge of community pharmacists regarding medications for SUD and evaluate their attitudes, levels of stigma, and clinical practices in SUD. Methods: A questionnaire was developed to assess practices, knowledge, screening services, and attitudes toward harm reduction strategies and treatment. A standardized measure of stigma was included along with demographics. A cross-sectional electronic survey was conducted in Pennsylvania, Ohio, and West Virginia among a non-probability sample of community pharmacists working for a retail pharmacy chain (n = 910) and a local alumni network (n = 50). Scores were calculated for each factor and descriptive analyses, mean differences (t-tests and ANOVA), correlations with demographics and practice characteristics were performed. Linear and ordinal regressions were utilized to predict knowledge, practice, screening, and stigma scores. Results: A total of 134 responses (response rate 13.9%) were collected. On average, the pharmacists were 38 years old, had worked for 15 years, primarily full-time with practice locations in suburban settings. Only 53% reported they received SUD education in pharmacy school. Pharmacists received a mean score of 5.5 and 3.5 out of eight and seven on knowledge and practice scales, respectively. Pharmacists overall had slightly stigmatizing and negative attitudes, with higher stigma significantly related to performing lesser services and considering screenings as important. Number of years worked significantly predicted knowledge and screening. Conclusion: Relationships between knowledge, attitudes, and practices indicate a need for experiential education that includes psychosocial aspects of care with increased opportunities for practice.", "gemini": "Bối cảnh: Dược sĩ cộng đồng có thể đóng một vai trò có ý nghĩa trong việc xác định và điều trị các rối loạn sử dụng chất gây nghiện (SUD). Tuy nhiên, kiến thức không đầy đủ về bệnh và thái độ tiêu cực là những rào cản đã biết. Mối quan hệ giữa kiến thức, thái độ và thực hành của dược sĩ đối với những người mắc SUD chưa được đánh giá toàn diện tại Hoa Kỳ. Mục tiêu của nghiên cứu là đánh giá kiến thức của dược sĩ cộng đồng về các loại thuốc điều trị SUD và đánh giá thái độ, mức độ kỳ thị và thực hành lâm sàng của họ trong SUD. Phương pháp: Một bảng câu hỏi đã được phát triển để đánh giá các thực hành, kiến thức, dịch vụ sàng lọc và thái độ đối với các chiến lược giảm tác hại và điều trị. Một biện pháp tiêu chuẩn về kỳ thị đã được đưa vào cùng với thông tin nhân khẩu học. Một cuộc khảo sát điện tử cắt ngang đã được tiến hành tại Pennsylvania, Ohio và West Virginia trong số một mẫu không xác suất gồm các dược sĩ cộng đồng làm việc cho một chuỗi nhà thuốc bán lẻ (n = 910) và một mạng lưới cựu sinh viên địa phương (n = 50). Điểm số được tính cho từng yếu tố và các phân tích mô tả, chênh lệch trung bình (kiểm định t và ANOVA), tương quan với các đặc điểm nhân khẩu học và thực hành đã được thực hiện. Các hồi quy tuyến tính và thứ tự đã được sử dụng để dự đoán điểm số về kiến thức, thực hành, sàng lọc và kỳ thị. Kết quả: Tổng cộng có 134 phản hồi (tỷ lệ phản hồi 13,9%) đã được thu thập. Trung bình, các dược sĩ 38 tuổi, đã làm việc trong 15 năm, chủ yếu làm toàn thời gian với địa điểm hành nghề ở vùng ngoại ô. Chỉ có 53% báo cáo rằng họ đã nhận được giáo dục về SUD tại trường dược. Dược sĩ nhận được điểm trung bình là 5,5 và 3,5 trên tám và bảy về thang điểm kiến thức và thực hành. Nhìn chung, các dược sĩ có thái độ hơi kỳ thị và tiêu cực, với sự kỳ thị cao hơn có liên quan đáng kể đến việc thực hiện ít dịch vụ hơn và coi việc sàng lọc là quan trọng. Số năm làm việc dự đoán đáng kể kiến thức và sàng lọc. Kết luận: Mối quan hệ giữa kiến thức, thái độ và thực hành chỉ ra sự cần thiết của giáo dục theo kinh nghiệm bao gồm các khía cạnh tâm lý xã hội của việc chăm sóc với nhiều cơ hội thực hành hơn."} +{"idx": 1870443, "en": "Ear rot is a globally prevalent class of disease in maize, of which Fusarium ear rot (FER), caused by the fungal pathogen Fusarium verticillioides, is the most commonly reported. In this study, three F2 populations, namely F2-C, F2-D, and F2-J, and their corresponding F2:3 families were produced by crossing three highly FER-resistant inbred lines, Cheng351, Dan598, and JiV203, with the same susceptible line, ZW18, for quantitative trait locus (QTL) mapping of FER resistance. The individual crop plants were inoculated with a spore suspension of the pathogen injected into the kernels of the maize ears. The broad-sense heritability (H) for FER resistance was estimated to be as high as 0.76, 0.81, and 0.78 in F2-C, F2-D, and F2-J, respectively, indicating that genetic factors played a key role in the phenotypic variation. We detected a total of 20 FER-resistant QTLs in the three F2 populations, among which QTLs derived from the resistant parent Cheng351, Dan598, and JiV203 explained 62.89 to 82.25%, 43.19 to 61.51%, and 54.70 to 75.77% of the phenotypic variation, respectively. Among all FER-resistant QTLs detected, qRfer1, qRfer10, and qRfer17 accounted for the phenotypic variation as high as 26.58 to 43.36%, 11.76 to 18.02%, and 12.02 to 21.81%, respectively. Furthermore, QTLs mapped in different F2 populations showed some extent of overlaps indicating potential resistance hotspots. The FER-resistant QTLs detected in this study can be explored as useful candidates to improve FER resistance in maize by introducing these QTLs into susceptible maize inbred lines via molecular marker-assisted selection.", "gemini": "Bệnh thối tai là một loại bệnh phổ biến trên toàn cầu ở ngô, trong đó bệnh thối tai do Fusarium (FER), do tác nhân gây bệnh nấm Fusarium verticillioides, được báo cáo phổ biến nhất. Trong nghiên cứu này, ba quần thể F2, cụ thể là F2-C, F2-D và F2-J, và các họ F2:3 tương ứng của chúng được tạo ra bằng cách lai ba dòng thuần chủng có khả năng kháng FER cao, Cheng351, Dan598 và JiV203, với cùng một dòng nhạy cảm, ZW18, để lập bản đồ gen định lượng (QTL) về khả năng kháng FER. Các cây trồng riêng lẻ được tiêm một hỗn dịch bào tử của tác nhân gây bệnh vào hạt của bắp ngô. Di truyền học phổ (H) đối với khả năng kháng FER được ước tính là cao tới 0,76, 0,81 và 0,78 ở F2-C, F2-D và F2-J, tương ứng, chỉ ra rằng các yếu tố di truyền đóng một vai trò quan trọng trong biến thể kiểu hình. Chúng tôi đã phát hiện tổng cộng 20 QTL kháng FER trong ba quần thể F2, trong số đó các QTL có nguồn gốc từ cha mẹ kháng Cheng351, Dan598 và JiV203 giải thích từ 62,89 đến 82,25%, 43,19 đến 61,51% và 54,70 đến 75,77% biến thể kiểu hình, tương ứng. Trong số tất cả các QTL kháng FER được phát hiện, qRfer1, qRfer10 và qRfer17 chiếm biến thể kiểu hình cao tới 26,58 đến 43,36%, 11,76 đến 18,02% và 12,02 đến 21,81%, tương ứng. Hơn nữa, các QTL được lập bản đồ trong các quần thể F2 khác nhau cho thấy một số mức độ chồng chéo chỉ ra các điểm nóng kháng tiềm ẩn. Các QTL kháng FER được phát hiện trong nghiên cứu này có thể được khám phá như những ứng cử viên hữu ích để cải thiện khả năng kháng FER ở ngô bằng cách đưa các QTL này vào các dòng thuần chủng ngô nhạy cảm thông qua chọn lọc hỗ trợ đánh dấu phân tử."} +{"idx": 1870444, "en": "Fungi in the genus Colletotrichum cause apple, blueberry, and strawberry fruit rots, which can result in significant losses. Accurate identification is important because species differ in aggressiveness, fungicide sensitivity, and other factors affecting management. Multiple Colletotrichum species can cause similar symptoms on the same host, and more than one fruit type can be infected by a single Colletotrichum species. Mixed-fruit orchards may facilitate cross-infection, with significant management implications. Colletotrichum isolates from small fruits in Kentucky orchards were characterized and compared with apple isolates via a combination of morphotyping, sequencing of voucher loci and whole genomes, and cross-inoculation assays. Seven morphotypes representing two species complexes (C. acutatum and C. gloeosporioides) were identified. Morphotypes corresponded with phylogenetic species C. fioriniae, C. fructicola, C. nymphaeae, and C. siamense, identified by TUB2 or GAPDH barcodes. Phylogenetic trees built from nine single-gene sequences matched barcoding results with one exception, later determined to belong to an undescribed species. Comparison of single-gene trees with representative whole genome sequences revealed that CHS and ApMat were the most informative for diagnosis of fruit rot species and individual morphotypes within the C. acutatum or C. gloeosporioides complexes, respectively. All blueberry isolates belonged to C. fioriniae, and most strawberry isolates were C. nymphaeae, with a few C. siamense and C. fioriniae also recovered. All three species cause fruit rot on apples in Kentucky. Cross-inoculation assays on detached apple, blueberry, and strawberry fruits showed that all species were pathogenic on all three hosts but with species-specific differences in aggressiveness.", "gemini": "Nấm trong chi Colletotrichum gây thối quả táo, việt quất và dâu tây, có thể dẫn đến thiệt hại đáng kể. Việc xác định chính xác rất quan trọng vì các loài khác nhau về mức độ gây hại, độ nhạy cảm với thuốc diệt nấm và các yếu tố khác ảnh hưởng đến công tác quản lý. Nhiều loài Colletotrichum có thể gây ra các triệu chứng tương tự trên cùng một vật chủ và nhiều loại trái cây có thể bị nhiễm bởi một loài Colletotrichum duy nhất. Các vườn cây ăn quả hỗn hợp có thể tạo điều kiện thuận lợi cho sự lây nhiễm chéo, với những ý nghĩa quan trọng trong công tác quản lý. Các phân lập Colletotrichum từ các loại quả nhỏ trong các vườn cây ở Kentucky đã được đặc trưng và so sánh với các phân lập từ táo thông qua sự kết hợp của phép phân tích hình thái, giải trình tự các gen đánh dấu và toàn bộ hệ gen, cũng như các xét nghiệm tiêm chủng chéo. Bảy kiểu hình đại diện cho hai phức hợp loài (C. acutatum và C. gloeosporioides) đã được xác định. Các kiểu hình tương ứng với các loài phát sinh loài C. fioriniae, C. fructicola, C. nymphaeae và C. siamense, được xác định bằng mã vạch TUB2 hoặc GAPDH. Cây phát sinh loài được xây dựng từ chín trình tự gen đơn khớp với kết quả mã vạch với một ngoại lệ, sau đó được xác định là thuộc về một loài chưa được mô tả. So sánh các cây gen đơn với các trình tự toàn bộ hệ gen đại diện cho thấy CHS và ApMat là thông tin nhất để chẩn đoán các loài gây thối quả và các kiểu hình riêng lẻ trong các phức hợp C. acutatum hoặc C. gloeosporioides. Tất cả các phân lập từ việt quất đều thuộc về C. fioriniae và hầu hết các phân lập từ dâu tây là C. nymphaeae, với một số ít C. siamense và C. fioriniae cũng được phục hồi. Cả ba loài đều gây thối quả trên táo ở Kentucky. Các xét nghiệm tiêm chủng chéo trên quả táo, việt quất và dâu tây đã tách ra cho thấy rằng tất cả các loài đều gây bệnh trên cả ba vật chủ nhưng có sự khác biệt về mức độ gây hại tùy theo từng loài."} +{"idx": 1870445, "en": "This article provides an up-to-date review of disease-causing viruses and phytoplasmas of currants including symptoms, transmission, detection, economic impact, and control measures. Currants are widely cultivated in more than 30 countries in the temperate zones of Europe, Asia, South America, Australia, and New Zealand. Ribes spp. can be infected by more than 20 virus species and four Candidatus Phytoplasma species, with more to be described in the future. High-throughput sequencing opened a new era of deciphering virus variants and mixed infections, leading to the characterization of several new species. The use of clean propagation material is the basis for control of Ribes graft-transmissible diseases, but this has become a challenging task given the ever-growing number of newly discovered pathogens.", "gemini": "Bài viết này cung cấp một đánh giá mới nhất về các loại vi-rút và vi khuẩn gây bệnh ở cây lý chua bao gồm các triệu chứng, cách lây truyền, phát hiện, tác động kinh tế và biện pháp kiểm soát. Cây lý chua được trồng rộng rãi ở hơn 30 quốc gia ở các vùng ôn đới của Châu Âu, Châu Á, Nam Mỹ, Châu Úc và New Zealand. Ribes spp. có thể bị nhiễm bởi hơn 20 loài vi-rút và bốn loài Candidatus Phytoplasma, với nhiều loài khác sẽ được mô tả trong tương lai. Trình tự thông lượng cao đã mở ra một kỷ nguyên mới để giải mã các biến thể vi-rút và các bệnh nhiễm trùng hỗn hợp, dẫn đến việc mô tả đặc điểm của một số loài mới. Việc sử dụng vật liệu nhân giống sạch là cơ sở để kiểm soát các bệnh lây truyền qua ghép ở Ribes, nhưng đây đã trở thành một nhiệm vụ đầy thách thức do số lượng các tác nhân gây bệnh mới được phát hiện ngày càng tăng."} +{"idx": 1870446, "en": "Root rot caused by Fusarium species is a major problem in the pulse growing regions of Montana. Fusarium isolates (n = 112) were obtained from seeds and roots of chickpea, dry pea, and lentil. Isolates were identified by comparing the sequences of the internal transcribed spacer region and the translation elongation factor 1-α in Fusarium-ID database. Fusarium avenaceum was the most abundant species (28%), followed by F. acuminatum (21%), F. poae (13%), F. oxysporum (8%), F. culmorum (6%), F. redolens (6%), F. sporotrichioides (6%), F. solani (4%), F. graminearum (2%), F. torulosum (2%), and F. tricinctum (0.9%). The aggressiveness of a subset of 50 isolates that represent various sources of isolation was tested on three pulse crops and two cereal crops. Nonparametric analysis of variance conducted on ranks of disease severity indicated that F. avenaceum and F. solani isolates were highly aggressive on pea and chickpea. In lentil, F. avenaceum and F. culmorum were highly aggressive. In barley, F. avenaceum, F. solani, F. culmorum, and F. graminearum were highly aggressive. In wheat, F. avenaceum, F. graminearum, and F. culmorum were highly aggressive. Two F. avenaceum isolates were highly aggressive across all the crops tested and found to be cross-pathogenic. One isolate of F. culmorum and an isolate of F. graminearum obtained from chickpea and lentil seed were highly aggressive on barley and wheat. The results indicate that multiple Fusarium spp. from seeds and roots can cause root rot on both pulse and cereal crops. Rotating these crops may still lead to an increase in inoculum levels, making crop rotation limited in efficacy as a disease management strategy.", "gemini": "Bệnh thối rễ do loài Fusarium gây ra là một vấn đề lớn ở các vùng trồng cây họ đậu ở Montana. Các phân lập Fusarium (n = 112) thu được từ hạt và rễ của cây đậu gà, đậu Hà Lan khô và đậu lăng. Các phân lập đã được xác định bằng cách so sánh các trình tự của vùng lặp lại phiên mã bên trong và yếu tố kéo dài dịch mã 1-α trong cơ sở dữ liệu Fusarium-ID. Fusarium avenaceum là loài phổ biến nhất (28%), tiếp theo là F. acuminatum (21%), F. poae (13%), F. oxysporum (8%), F. culmorum (6%), F. redolens (6%), F. sporotrichioides (6%), F. solani (4%), F. graminearum (2%), F. torulosum (2%) và F. tricinctum (0,9%). Mức độ gây hại của một tập hợp con gồm 50 phân lập đại diện cho nhiều nguồn phân lập khác nhau đã được thử nghiệm trên ba loại cây họ đậu và hai loại cây ngũ cốc. Phân tích phương sai không tham số được thực hiện trên thứ hạng mức độ nghiêm trọng của bệnh cho thấy các phân lập F. avenaceum và F. solani có mức độ gây hại cao đối với đậu Hà Lan và đậu gà. Ở đậu lăng, F. avenaceum và F. culmorum có mức độ gây hại cao. Ở lúa mạch, F. avenaceum, F. solani, F. culmorum và F. graminearum có mức độ gây hại cao. Ở lúa mì, F. avenaceum, F. graminearum và F. culmorum có mức độ gây hại cao. Hai phân lập F. avenaceum có mức độ gây hại cao trên tất cả các loại cây trồng được thử nghiệm và được phát hiện là có khả năng gây bệnh chéo. Một phân lập F. culmorum và một phân lập F. graminearum thu được từ hạt đậu gà và đậu lăng có mức độ gây hại cao trên lúa mạch và lúa mì. Kết quả cho thấy rằng nhiều loài Fusarium spp. từ hạt và rễ có thể gây thối rễ trên cả cây họ đậu và cây ngũ cốc. Việc luân canh các loại cây trồng này vẫn có thể dẫn đến sự gia tăng mức độ inôculô, khiến cho việc luân canh cây trồng bị hạn chế về hiệu quả như một chiến lược quản lý bệnh."} +{"idx": 1870447, "en": "OBJECTIVE Performing tracheotomy in patients with COVID-19 carries a risk of transmission to the surgical team due to potential viral particle aerosolization. Few studies have reported transmission rates to tracheotomy surgeons. We describe our safety practices and the transmission rate to our surgical team after performing tracheotomy on patients with COVID-19 during the peak of the pandemic at a US epicenter. STUDY DESIGN Retrospective cohort study. SETTING Tertiary academic hospital. METHODS Tracheotomy procedures for patients with COVID-19 that were performed April 15 to May 28, 2020, were reviewed, with a focus on the surgical providers involved. Methods of provider protection were recorded. Provider health status was the main outcome measure. RESULTS Thirty-six open tracheotomies were performed, amounting to 65 surgical provider exposures, and 30 (83.3%) procedures were performed at bedside. The mean time to tracheotomy from hospital admission for SARS-CoV-2 symptoms was 31 days, and the mean time to intubation was 24 days. Standard personal protective equipment, according to Centers for Disease Control and Prevention, was worn for each case. Powered air-purifying respirators were not used. None of the surgical providers involved in tracheotomy for patients with COVID-19 demonstrated positive antibody seroconversion or developed SARS-CoV-2-related symptoms to date. CONCLUSION Tracheotomy for patients with COVID-19 can be done with minimal risk to the surgical providers when standard personal protective equipment is used (surgical gown, gloves, eye protection, hair cap, and N95 mask). Whether timing of tracheotomy following onset of symptoms affects the risk of transmission needs further study.", "gemini": "MỤC TIÊU Thực hiện phẫu thuật mở khí quản cho những bệnh nhân mắc COVID-19 có nguy cơ lây truyền cho nhóm phẫu thuật do khả năng tạo thành hạt nhân virus trong không khí. Một số ít nghiên cứu đã báo cáo tỷ lệ lây truyền cho các bác sĩ phẫu thuật mở khí quản. Chúng tôi mô tả các biện pháp an toàn và tỷ lệ lây truyền cho nhóm phẫu thuật sau khi thực hiện phẫu thuật mở khí quản cho những bệnh nhân mắc COVID-19 trong thời kỳ đỉnh dịch tại một trung tâm dịch bệnh ở Hoa Kỳ. THIẾT KẾ NGHIÊN CỨU Nghiên cứu theo dõi hồi cứu. ĐỊA ĐIỂM Bệnh viện đa khoa bậc ba. PHƯƠNG PHÁP Các thủ thuật mở khí quản cho những bệnh nhân mắc COVID-19 được thực hiện từ ngày 15 tháng 4 đến ngày 28 tháng 5 năm 2020 đã được xem xét, tập trung vào các nhà cung cấp dịch vụ phẫu thuật liên quan. Các phương pháp bảo vệ nhà cung cấp đã được ghi lại. Tình trạng sức khỏe của nhà cung cấp là biện pháp đánh giá kết quả chính. KẾT QUẢ Đã thực hiện 36 ca mở khí quản, tương đương với 65 lần tiếp xúc của nhà cung cấp dịch vụ phẫu thuật và 30 (83,3%) thủ thuật được thực hiện tại giường. Thời gian trung bình để mở khí quản kể từ khi nhập viện do các triệu chứng SARS-CoV-2 là 31 ngày và thời gian trung bình để đặt nội khí quản là 24 ngày. Thiết bị bảo vệ cá nhân tiêu chuẩn, theo Trung tâm Kiểm soát và Phòng ngừa Dịch bệnh, đã được sử dụng cho từng trường hợp. Máy thở lọc không khí chạy bằng điện không được sử dụng. Cho đến nay, không có nhà cung cấp dịch vụ phẫu thuật nào tham gia mở khí quản cho những bệnh nhân mắc COVID-19 có kết quả xét nghiệm huyết thanh dương tính hoặc phát triển các triệu chứng liên quan đến SARS-CoV-2. KẾT LUẬN Có thể mở khí quản cho những bệnh nhân mắc COVID-19 với nguy cơ tối thiểu cho các nhà cung cấp dịch vụ phẫu thuật khi sử dụng thiết bị bảo vệ cá nhân tiêu chuẩn (áo phẫu thuật, găng tay, bảo vệ mắt, mũ trùm đầu và khẩu trang N95). Việc mở khí quản sau khi khởi phát các triệu chứng có ảnh hưởng đến nguy cơ lây truyền hay không cần được nghiên cứu thêm."} +{"idx": 1870448, "en": "Little is known about our autobiographical memories for cultural events. This represents an opportunity for cultural institutions such as museums, as examination of visitor memories is one way in which they can seek to understand the long-term impact they may have on their visitors. This research applied a coding model developed from autobiographical memory theory to analysis of participants' memories for museum visits, considering the distribution of memories across the life span, types of memories and content. Differences between visitor groups (age, visit frequency) were also considered. Findings showed a strong recency effect in the life-span distribution, suggesting the importance of social sharing in memories of cultural experience. Analysis of content showed a hierarchy of information that was present in museum memories. Knowledge acquired during the event of the visit was important, as was contextualising information whereby visitors situated the memory within their autobiographical knowledge and chronology. Emotions and thoughts were also salient. Visitor differences had minimal impact on content, with the exception of some effects that were consistent with the literature on memory and ageing. This research develops understanding of autobiographical memories for cultural experiences and provides insight to museums, with practical implications in terms of understanding visitors' experiences.", "gemini": "Chúng ta biết rất ít về những ký ức tự truyện của mình đối với các sự kiện văn hóa. Đây là một cơ hội cho các tổ chức văn hóa như bảo tàng, vì việc kiểm tra ký ức của du khách là một cách để họ có thể tìm cách hiểu được tác động lâu dài mà họ có thể có đối với những du khách của mình. Nghiên cứu này đã áp dụng một mô hình mã hóa được phát triển từ lý thuyết về trí nhớ tự truyện để phân tích ký ức của những người tham gia về các chuyến thăm bảo tàng, xem xét sự phân bố của ký ức trong suốt cuộc đời, các loại ký ức và nội dung. Sự khác biệt giữa các nhóm du khách (tuổi tác, tần suất ghé thăm) cũng được xem xét. Kết quả cho thấy hiệu ứng gần đây mạnh mẽ trong phân bố suốt đời, cho thấy tầm quan trọng của việc chia sẻ xã hội trong ký ức về trải nghiệm văn hóa. Phân tích nội dung cho thấy một hệ thống phân cấp thông tin có trong ký ức của bảo tàng. Kiến thức thu được trong sự kiện của chuyến thăm rất quan trọng, cũng như thông tin ngữ cảnh mà du khách đặt ký ức trong kiến thức và niên biểu tự truyện của họ. Cảm xúc và suy nghĩ cũng rất nổi bật. Sự khác biệt của du khách có tác động tối thiểu đến nội dung, ngoại trừ một số hiệu ứng phù hợp với các tài liệu về trí nhớ và lão hóa. Nghiên cứu này phát triển sự hiểu biết về ký ức tự truyện đối với các trải nghiệm văn hóa và cung cấp thông tin chi tiết cho các bảo tàng, với những hàm ý thực tế về việc hiểu trải nghiệm của du khách."} +{"idx": 1870449, "en": "INTRODUCTION The development of immune-checkpoint inhibitors targeting the programmed death-1 (PD-1) and its ligand (PD-L1) axis has transformed the treatment paradigm in non-small-cell lung cancer, bringing about unprecedented 5-year survival rates. Despite this dramatic improvement, roughly 70% of patients do not derive durable benefit from these treatments, illustrating the need for predictive biomarkers. AREAS COVERED In this review, we will discuss what makes a successful biomarker and analyze the role and significance of currently available options, including PD-L1, oncogenic alterations and tumor mutation burden. We then discuss potential biomarkers on the horizon, including the microbiome, tumor infiltrating lymphocytes, neutrophil-to-lymphocyte ratio, gene signatures and the emerging field of multiomics. EXPERT OPINION To date, only PD-L1 is clinically validated as a positive predictor of response to immunotherapy, yet the need to refine patient selection has never been stronger, given the indication for checkpoint inhibitors alone or in combination in all non-oncogene driven non-small-cell lung cancer patients receiving front-line therapy. Prospective validation of the above-mentioned potential biomarkers, either alone or in combination, may help to elaborate improved predictive tools.", "gemini": "GIỚI THIỆU Sự phát triển của các chất ức chế điểm kiểm miễn dịch nhắm vào trục tế bào chết theo chương trình-1 (PD-1) và phối tử của nó (PD-L1) đã làm thay đổi mô hình điều trị ung thư phổi không phải tế bào nhỏ, mang lại tỷ lệ sống sót 5 năm chưa từng có. Mặc dù có sự cải thiện đáng kể này, nhưng khoảng 70% bệnh nhân không có được lợi ích lâu dài từ các phương pháp điều trị này, minh họa cho nhu cầu về các dấu ấn sinh học dự báo. CÁC LĨNH VỰC ĐƯỢC BAO PHỦ Trong bài đánh giá này, chúng tôi sẽ thảo luận về những gì tạo nên một dấu ấn sinh học thành công và phân tích vai trò và ý nghĩa của các lựa chọn hiện có, bao gồm PD-L1, biến đổi ung thư và gánh nặng đột biến khối u. Sau đó, chúng tôi thảo luận về các dấu ấn sinh học tiềm năng trong tương lai, bao gồm hệ vi sinh vật, tế bào lympho xâm nhập khối u, tỷ lệ bạch cầu trung tính trên bạch cầu, chữ ký gen và lĩnh vực đa dạng mới nổi. Ý KIẾN CỦA CHUYÊN GIA Cho đến nay, chỉ có PD-L1 được xác nhận lâm sàng là một yếu tố dự báo dương tính về đáp ứng với liệu pháp miễn dịch, nhưng nhu cầu tinh chỉnh lựa chọn bệnh nhân chưa bao giờ mạnh mẽ hơn, vì chỉ định sử dụng chất ức chế điểm kiểm riêng lẻ hoặc kết hợp ở tất cả bệnh nhân ung thư phổi không phải tế bào nhỏ không do ung thư gây ra đang được điều trị tuyến đầu. Việc xác nhận giá trị dự đoán của các dấu ấn sinh học tiềm năng được đề cập ở trên, dù đơn lẻ hay kết hợp, có thể giúp xây dựng các công cụ dự đoán được cải thiện."} +{"idx": 1870450, "en": "Methamphetamine use disorder (MUD) frequently begins in adolescence, often accompanied by other psychiatric or mental disorders. Up to now, no comprehensive review about MUD and comorbid disorders in adolescents is available. We thus aimed to review the literature on comorbid mental disorders and MUD in adolescents in order to identify future research topics. Method: A PubMed search was conducted in July 2019. Relevant comorbidities were defined as attention-deficit disorder with/without hyperactivity, anxiety disorders, depression, eating disorders, post-traumatic stress disorder, psychosis, borderline personality disorder, conduct disorder and antisocial personality disorder, as well as other substance use disorders. For each comorbidity, we summarized prevalence rates, findings on comorbidity mechanisms, and recommended treatment options, if applicable. Results: Few articles focused on MUD in adolescents. Prevalence rates differed largely between comorbid disorders, with tobacco use disorder, conduct disorder, post-traumatic stress disorder, anxiety disorders, and attention-deficit disorders being the most prevalent comorbidities while eating disorders were rare. Examined onset patterns and comorbidity mechanisms indicated three groups of comorbidities: preexisting disorders self-medicated with methamphetamine, disorders induced by chronic methamphetamine use, and disorders arising due to risk factors shared with MUD. Reviewed comorbidities were frequently associated with worse treatment outcomes. Conclusions: The limited evidence is in stark contrast to the presumably high prevalence and relevance of comorbid mental disorders in adolescents with MUD. Suggestions for future research topics, informed by adult findings, include genetic vulnerabilities, biological changes, and consequences of different use patterns. Surprisingly few MUD treatment programs explicitly integrate comorbid mental disorder modules.", "gemini": "Rối loạn sử dụng methamphetamine (MUD) thường bắt đầu ở tuổi vị thành niên, thường đi kèm với các rối loạn tâm thần hoặc rối loạn tâm lý khác. Cho đến nay, không có đánh giá toàn diện nào về MUD và các rối loạn đi kèm ở thanh thiếu niên. Do đó, chúng tôi muốn xem xét các tài liệu về các rối loạn tâm thần đi kèm và MUD ở thanh thiếu niên để xác định các chủ đề nghiên cứu trong tương lai. Phương pháp: Một tìm kiếm PubMed đã được thực hiện vào tháng 7 năm 2019. Các bệnh đi kèm có liên quan được định nghĩa là rối loạn thiếu chú ý có/không có tăng động, rối loạn lo âu, trầm cảm, rối loạn ăn uống, rối loạn căng thẳng sau chấn thương, loạn thần, rối loạn nhân cách ranh giới, rối loạn hành vi và rối loạn nhân cách chống đối xã hội, cũng như các rối loạn sử dụng chất khác. Đối với mỗi bệnh đi kèm, chúng tôi đã tóm tắt tỷ lệ mắc, các phát hiện về cơ chế bệnh đi kèm và các lựa chọn điều trị được khuyến nghị, nếu có. Kết quả: Ít bài báo tập trung vào MUD ở thanh thiếu niên. Tỷ lệ mắc khác nhau rất lớn giữa các rối loạn đi kèm, trong đó rối loạn sử dụng thuốc lá, rối loạn hành vi, rối loạn căng thẳng sau chấn thương, rối loạn lo âu và rối loạn thiếu chú ý là những bệnh đi kèm phổ biến nhất trong khi rối loạn ăn uống rất hiếm. Các kiểu khởi phát và cơ chế bệnh đi kèm được kiểm tra cho thấy ba nhóm bệnh đi kèm: các rối loạn đã có từ trước được tự điều trị bằng methamphetamine, các rối loạn do sử dụng methamphetamine mạn tính và các rối loạn phát sinh do các yếu tố rủi ro chung với MUD. Các bệnh đi kèm được xem xét thường liên quan đến kết quả điều trị kém hơn. Kết luận: Bằng chứng hạn chế trái ngược hoàn toàn với tỷ lệ mắc cao và có liên quan của các rối loạn tâm thần đi kèm ở thanh thiếu niên mắc MUD. Các đề xuất cho các chủ đề nghiên cứu trong tương lai, được thông báo bởi các phát hiện ở người lớn, bao gồm các lỗ hổng về di truyền, các thay đổi sinh học và hậu quả của các kiểu sử dụng khác nhau. Đáng ngạc nhiên là có rất ít chương trình điều trị MUD tích hợp rõ ràng các mô-đun rối loạn tâm thần đi kèm."} +{"idx": 1870451, "en": "OBJECTIVES Little is known about the cardiotoxic effects of kratom (Mitragyna speciosa Korth.), a medicinal plant. This analytical cross-sectional study investigated the prevalence of electrocardiogram (ECG) abnormalities and QTc intervals in regular kratom users compared with non-kratom-using control subjects. METHODS We enrolled regular kratom users and non-kratom-using control subjects from three communities. Demographic data, clinical data, kratom use characteristics, and ECG findings were recorded. The mitragynine content of kratom juice was quantified using a validated gas chromatography-mass spectrometry (GC-MS) method. RESULTS A total of 200 participants (100 kratom users and 100 control subjects) participated in this study. The prevalence of ECG abnormalities in kratom users (28%) did not differ from that of control subjects (32%). Kratom use was not associated with ECG abnormalities, except for significantly higher odds of sinus tachycardia (OR = 8.61, 95% CI = 1.06-70.17, p = 0.035) among kratom users compared with control subjects. The odds of observing borderline QTc intervals were significantly higher for kratom users compared with control subjects, regardless of the age of first use, the duration of use, the daily quantity consumed, and the length of time that had elapsed between last kratom use and ECG assessment. Nevertheless, there were no differences in the odds of having prolonged QTc intervals between kratom users and controls. The estimated average daily intake of mitragynine consumed by kratom users was 434.28 mg. CONCLUSION We found no link between regular kratom use and electrocardiographic abnormalities with an estimated average daily intake of 434.28 mg of mitragynine.", "gemini": "MỤC TIÊU Người ta biết rất ít về các tác động độc hại đối với tim của kratom (Mitragyna speciosa Korth.), một loại cây thuốc. Nghiên cứu cắt ngang phân tích này đã điều tra tỷ lệ mắc các bất thường trên điện tâm đồ (ECG) và khoảng QTc ở những người sử dụng kratom thường xuyên so với những đối tượng đối chứng không sử dụng kratom. PHƯƠNG PHÁP Chúng tôi đã tuyển dụng những người sử dụng kratom thường xuyên và những đối tượng đối chứng không sử dụng kratom từ ba cộng đồng. Dữ liệu nhân khẩu học, dữ liệu lâm sàng, đặc điểm sử dụng kratom và kết quả ECG đã được ghi lại. Hàm lượng mitragynine trong nước kratom được định lượng bằng phương pháp sắc ký khí-phổ khối (GC-MS) đã được xác nhận. KẾT QUẢ Tổng cộng có 200 người tham gia (100 người sử dụng kratom và 100 đối tượng đối chứng) đã tham gia nghiên cứu này. Tỷ lệ mắc các bất thường trên ECG ở những người sử dụng kratom (28%) không khác so với những đối tượng đối chứng (32%). Việc sử dụng kratom không liên quan đến các bất thường trên ECG, ngoại trừ tỷ lệ nhịp tim nhanh xoang cao hơn đáng kể (OR = 8,61, KTC 95% = 1,06-70,17, p = 0,035) ở những người sử dụng kratom so với những đối tượng đối chứng. Tỷ lệ quan sát thấy khoảng QTc ở mức ranh giới cao hơn đáng kể ở những người sử dụng kratom so với những đối tượng đối chứng, bất kể tuổi bắt đầu sử dụng, thời gian sử dụng, lượng tiêu thụ hàng ngày và khoảng thời gian trôi qua giữa lần sử dụng kratom cuối cùng và đánh giá ECG. Tuy nhiên, không có sự khác biệt về tỷ lệ có khoảng QTc kéo dài giữa những người sử dụng kratom và những người đối chứng. Lượng mitragynine trung bình ước tính hàng ngày mà những người sử dụng kratom tiêu thụ là 434,28 mg. KẾT LUẬN Chúng tôi không tìm thấy mối liên hệ nào giữa việc sử dụng kratom thường xuyên và các bất thường trên điện tâm đồ với lượng mitragynine trung bình ước tính hàng ngày là 434,28 mg."} +{"idx": 1870452, "en": "Background: Understanding the impact of medications for opioid use disorder on health related quality of life (QOL) may help to explain why few individuals with legal involvement remain in treatment, specifically those receiving opioid antagonists. QOL is an established predictor of treatment retention and has been shown to improve with some treatment for opioid use disorder. Yet limited research has examined QOL with opioid antagonists. We examined the impact of extended release naltrexone (XR-NTX) on QOL and retention in treatment in a randomized, multi-site trial of individuals with legal involvement. Methods: The participants were 308 community-dwelling adults with current or recent legal involvement with opioid dependence at five site across United States. They were randomized to receive XR-NTX or treatment as usual for 6 months. QOL was measured every 2 weeks using Euro QOL individual items, summary index score, and health state today metric. Results: No significant difference in QOL scores were observed between the two groups at the completion of active treatment or on follow up at 52 and 78 weeks. There were no time effects of treatment on scores. Contrary to expectation, baseline and average QOL did not predict retention in treatment. Conclusion: In contrast to prior research, our findings did not demonstrate significant changes (improvements or decreases) in QOL associated with XR-NTX treatment. Clinicians may consider that individuals receiving XR-NTX may not experience changes in perceived well-being in response to treatment and consider discussing with patients that they may not necessarily perceive improvement in their QOL. This may help to ground patient's expectations about the effects of treatment and potentially reduce attrition from treatment with opioid antagonists.", "gemini": "Bối cảnh: Việc hiểu được tác động của các loại thuốc điều trị rối loạn sử dụng opioid đối với chất lượng cuộc sống liên quan đến sức khỏe (QOL) có thể giúp giải thích tại sao ít cá nhân có liên quan pháp lý vẫn tiếp tục điều trị, đặc biệt là những người dùng thuốc đối kháng opioid. QOL là một yếu tố dự báo đã được xác định của việc duy trì điều trị và đã được chứng minh là cải thiện với một số phương pháp điều trị rối loạn sử dụng opioid. Tuy nhiên, nghiên cứu hạn chế đã xem xét QOL với thuốc đối kháng opioid. Chúng tôi đã xem xét tác động của naltrexone giải phóng kéo dài (XR-NTX) đối với QOL và việc duy trì điều trị trong một thử nghiệm đa địa điểm, ngẫu nhiên ở những cá nhân có liên quan pháp lý. Phương pháp: Những người tham gia là 308 người lớn sống trong cộng đồng có liên quan pháp lý hiện tại hoặc gần đây với chứng phụ thuộc opioid tại năm địa điểm trên khắp Hoa Kỳ. Họ được phân ngẫu nhiên để dùng XR-NTX hoặc điều trị như thường lệ trong 6 tháng. QOL được đo 2 tuần một lần bằng cách sử dụng các mục riêng lẻ của Euro QOL, điểm số chỉ số tóm tắt và số liệu tình trạng sức khỏe hiện tại. Kết quả: Không có sự khác biệt đáng kể nào về điểm QOL được quan sát thấy giữa hai nhóm khi hoàn thành điều trị tích cực hoặc theo dõi ở tuần thứ 52 và 78. Không có tác dụng theo thời gian của điều trị đối với điểm số. Trái với kỳ vọng, QOL ban đầu và trung bình không dự đoán được việc duy trì điều trị. Kết luận: Trái ngược với các nghiên cứu trước đây, các phát hiện của chúng tôi không chứng minh được những thay đổi đáng kể (cải thiện hoặc giảm) về QOL liên quan đến điều trị XR-NTX. Các bác sĩ lâm sàng có thể xem xét rằng những cá nhân dùng XR-NTX có thể không trải qua những thay đổi về cảm giác khỏe mạnh khi đáp ứng với điều trị và cân nhắc thảo luận với bệnh nhân rằng họ không nhất thiết phải nhận thấy sự cải thiện về QOL của mình. Điều này có thể giúp định hình kỳ vọng của bệnh nhân về tác dụng của điều trị và có khả năng làm giảm tỷ lệ bỏ cuộc điều trị bằng thuốc đối kháng opioid."} +{"idx": 1870453, "en": "COVID-19 has created new challenges and opportunities regarding the way in which programs and applicants will interact in the 2020-2021 otolaryngology residency match cycle. Social media and other virtual platforms offer a flexible and efficient medium for applicants and programs to gain information, communicate, and align interests. In this commentary, we explore ways in which social media may facilitate recruitment and networking in the virtual otolaryngology match.", "gemini": "COVID-19 đã tạo ra những thách thức và cơ hội mới liên quan đến cách thức các chương trình và ứng viên sẽ tương tác trong chu kỳ tuyển dụng nội trú về tai mũi họng năm 2020-2021. Phương tiện truyền thông xã hội và các nền tảng ảo khác cung cấp một phương tiện linh hoạt và hiệu quả để các ứng viên và chương trình thu thập thông tin, giao tiếp và thống nhất lợi ích. Trong bài bình luận này, chúng tôi khám phá các cách mà phương tiện truyền thông xã hội có thể tạo điều kiện thuận lợi cho việc tuyển dụng và kết nối trong chương trình tuyển dụng tai mũi họng ảo."} +{"idx": 1870454, "en": "Vicarious memories are memories that people have in reference to events that they have not directly experienced, rather heard second-hand. Little research has investigated vicarious memory; when it has, it has predominantly focused on vicarious trauma. The purpose of this study was to compare vicarious memories with personal memories. University students (N = 142) completed an in-person interview in which they recalled four memories: a highly positive personal memory, a highly negative personal memory, a highly positive vicarious memory and a highly negative vicarious memory. Personal and vicarious memory reports were compared and contrasted in terms of memory qualities, memory functions and event centrality. The results indicate that vicarious and personal memory reports share many phenomenological and functional properties. Although to a lesser degree than personal memories, vicarious memories do influence decision-making and problem-solving. Current models of episodic memory only include events that individuals have directly experienced. The current study adds to a growing body of literature which suggests that current models of episodic memory are too restrictive and should expand to include vicarious memory reports.", "gemini": "Ký ức gián tiếp là những ký ức mà mọi người có liên quan đến các sự kiện mà họ chưa trực tiếp trải qua, mà nghe lại từ người khác. Có rất ít nghiên cứu điều tra về ký ức gián tiếp; khi có, chủ yếu tập trung vào chấn thương gián tiếp. Mục đích của nghiên cứu này là so sánh ký ức gián tiếp với ký ức cá nhân. Sinh viên đại học (N = 142) đã hoàn thành một cuộc phỏng vấn trực tiếp, trong đó họ nhớ lại bốn ký ức: một ký ức cá nhân rất tích cực, một ký ức cá nhân rất tiêu cực, một ký ức gián tiếp rất tích cực và một ký ức gián tiếp rất tiêu cực. Các báo cáo về ký ức cá nhân và gián tiếp đã được so sánh và đối chiếu về mặt phẩm chất ký ức, chức năng ký ức và tính trung tâm của sự kiện. Kết quả cho thấy các báo cáo về ký ức gián tiếp và cá nhân có nhiều đặc tính hiện tượng học và chức năng chung. Mặc dù ở mức độ thấp hơn so với ký ức cá nhân, ký ức gián tiếp vẫn ảnh hưởng đến việc ra quyết định và giải quyết vấn đề. Các mô hình hiện tại của ký ức theo từng giai đoạn chỉ bao gồm các sự kiện mà cá nhân đã trực tiếp trải qua. Nghiên cứu hiện tại bổ sung vào một khối lượng lớn các tài liệu cho thấy rằng các mô hình hiện tại của ký ức theo từng giai đoạn quá hạn chế và nên mở rộng để bao gồm các báo cáo về ký ức gián tiếp."} +{"idx": 1870455, "en": "Virtual Reality (VR) can be used as a therapeutic tool to conduct efficacious in-session exposure therapy by presenting virtual equivalents of phobic stimuli, yet past hardware restrictions hindered implementation in routine care and effectiveness studies. The current study examines the effectiveness of a VR-assisted treatment protocol for public speaking anxiety with demonstrated efficacy, this time in routine care, using affordable VR hardware. Participants (n = 23) were recruited via a private clinic and treated by one of four psychologists with only minimal VR-training. Using a single-subject design and dual-slope modeling (adjusting the treatment-onset slope for treatment effects), we found a significant, large decrease in self-rated public speaking anxiety following the primary three-hour session, similar in magnitude to the previous efficacy trial. Multilevel modeling of in-session process measures suggests that the protocol works as intended, by decreasing catastrophic belief expectancy and distress, and increasing perceived performance quality. Adherence to the online transition program that followed-encouraging in-vivo exposure-was relatively poor, yet symptoms decrease continued. No change was observed over the three-month follow-up period. We conclude that VR exposure therapy can be effective under routine care conditions and is an attractive approach for future, large-scale implementation and effectiveness trials.", "gemini": "Thực tế ảo (VR) có thể được sử dụng như một công cụ điều trị để tiến hành liệu pháp tiếp xúc trong phiên hiệu quả bằng cách trình bày các tương đương ảo của kích thích gây ám ảnh, nhưng những hạn chế về phần cứng trước đây đã cản trở việc triển khai trong các nghiên cứu về chăm sóc thường quy và hiệu quả. Nghiên cứu hiện tại xem xét hiệu quả của một giao thức điều trị hỗ trợ VR cho chứng lo lắng khi nói trước công chúng với hiệu quả đã được chứng minh, lần này là trong chăm sóc thường quy, sử dụng phần cứng VR giá cả phải chăng. Những người tham gia (n = 23) được tuyển dụng thông qua một phòng khám tư và được điều trị bởi một trong bốn nhà tâm lý học chỉ được đào tạo tối thiểu về VR. Sử dụng thiết kế một đối tượng và mô hình độ dốc kép (điều chỉnh độ dốc bắt đầu điều trị cho các hiệu ứng điều trị), chúng tôi thấy rằng có sự giảm đáng kể, lớn về mức độ lo lắng khi nói trước công chúng tự đánh giá sau phiên ba giờ đầu tiên, tương tự về độ lớn so với thử nghiệm hiệu quả trước đó. Mô hình đa cấp các biện pháp quy trình trong phiên cho thấy rằng giao thức hoạt động như mong đợi, bằng cách giảm kỳ vọng niềm tin thảm khốc và sự đau khổ, và tăng chất lượng hiệu suất được nhận thức. Việc tuân thủ chương trình chuyển đổi trực tuyến theo sau khuyến khích tiếp xúc trong vivo tương đối kém, nhưng các triệu chứng vẫn tiếp tục giảm. Không có thay đổi nào được quan sát thấy trong giai đoạn theo dõi ba tháng. Chúng tôi kết luận rằng liệu pháp tiếp xúc VR có thể có hiệu quả trong các điều kiện chăm sóc thường quy và là một phương pháp tiếp cận hấp dẫn cho các thử nghiệm triển khai và hiệu quả quy mô lớn trong tương lai."} +{"idx": 1870456, "en": "We reviewed studies that have specifically explored the memory deficit hypothesis of Obsessive Compulsive Disorder (OCD) checking, highlighting the methodological differences between these studies that may explain inconsistencies regarding memory deficits in OCD checkers. Based on Conway's proposition that one function of episodic memories is to keep an adaptive record of recent goal processing in order to check that actions have actually been accomplished, we suggest that impaired autonoetic consciousness -one of the main features of episodic memory- may be at the heart of the issue of checking compulsion. Autonoetic consciousness, that can be experimentally assessed by the Remember/Know/Guess paradigm,could be impaired in OCD checkers leading them to be unable to mentally relive their actions in order to be assured that they have been accomplished (e.g., having locked the door). We make methodological suggestions to improve the assessment of autonoetic consciousness deficit in OCD checkers and understand its role in the etiology and maintenance of compulsive checking.", "gemini": "Chúng tôi đã xem xét các nghiên cứu cụ thể khám phá giả thuyết về sự thiếu hụt trí nhớ của hành vi kiểm tra của Rối loạn ám ảnh cưỡng chế (OCD), nêu bật sự khác biệt về phương pháp luận giữa các nghiên cứu này có thể giải thích sự không nhất quán về sự thiếu hụt trí nhớ ở những người kiểm tra OCD. Dựa trên đề xuất của Conway rằng một chức năng của ký ức theo từng giai đoạn là giữ lại bản ghi thích ứng về quá trình xử lý mục tiêu gần đây để kiểm tra xem các hành động đã thực sự được hoàn thành hay chưa, chúng tôi cho rằng ý thức tự chủ bị suy yếu - một trong những đặc điểm chính của trí nhớ theo từng giai đoạn - có thể là cốt lõi của vấn đề kiểm tra cưỡng chế. Ý thức tự chủ, có thể được đánh giá bằng thực nghiệm theo mô hình Nhớ/Biết/Đoán, có thể bị suy yếu ở những người kiểm tra OCD khiến họ không thể hồi tưởng lại các hành động của mình để đảm bảo rằng những hành động đó đã được hoàn thành (ví dụ: đã khóa cửa). Chúng tôi đưa ra các đề xuất về phương pháp luận để cải thiện việc đánh giá sự thiếu hụt ý thức tự chủ ở những người kiểm tra OCD và hiểu được vai trò của nó trong nguyên nhân và duy trì việc kiểm tra cưỡng chế."} +{"idx": 1870457, "en": "In this work, the ability of B12N12 fullerene-like nanoclusters as a drug carrier for isoniazid anti-tuberculosis drug has been studied by DFT methods. Binding energies in both gas and water phases are reported. The formed bonds between B12N12-FLN and Iso drug are studied and computed using QMAIM method. NPA is computed to obtain the total charges transferred in the B12N12-FLN-Iso drug complexes, NPA obtained values suggested that the cluster may oxidize the coordinated of Iso drug. The charge-transfer energy values are also computed and confirmed that the charges were transferred from the non-bonding lone-pair (n) of N and O atoms orbitals to the σ* orbitals of B and N atoms of B12N12-FLN. Also, the adsorption of Iso drug on BN nanoparticles surface (different sizes and shapes) and BN nanotubes was studied by Monte Carlo simulation. We found that increasing the BN size did not affect significantly on the adsorption energies of Iso drug for all various BN nanoparticles shapes. All adsorption energies obtained by MC calculations are negative values which revealed that the adsorption of the Iso drug molecule on BN surfaces is exothermic, spontaneous and energetically favourable. Also, the stability of B12N12-FLN-Iso drug complex in water explicitly was studied by MD simulations. MD simulation confirmed that iso-B12N12-FLN complexes are stable in the presence of water molecules. So, finally, we deduced that B12N12 fullerene-like nanoclusters can be acted as a drug carrier for isoniazid anti-tuberculosis drug. Communicated by Ramaswamy H. Sarma.", "gemini": "Trong công trình này, khả năng của các nano cụm giống fullerene B12N12 làm chất mang thuốc cho thuốc chống lao isoniazid đã được nghiên cứu bằng các phương pháp DFT. Năng lượng liên kết ở cả pha khí và pha nước đều được báo cáo. Các liên kết được hình thành giữa B12N12-FLN và thuốc Iso được nghiên cứu và tính toán bằng phương pháp QMAIM. NPA được tính toán để thu được tổng điện tích được chuyển trong các phức hợp thuốc B12N12-FLN-Iso, các giá trị thu được của NPA cho thấy cụm có thể oxy hóa thuốc Iso được phối hợp. Các giá trị năng lượng chuyển điện tích cũng được tính toán và xác nhận rằng các điện tích đã được chuyển từ các quỹ đạo cặp đơn không liên kết (n) của các nguyên tử N và O sang các quỹ đạo σ* của các nguyên tử B và N của B12N12-FLN. Ngoài ra, sự hấp phụ của thuốc Iso trên bề mặt các hạt nano BN (kích thước và hình dạng khác nhau) và các ống nano BN đã được nghiên cứu bằng mô phỏng Monte Carlo. Chúng tôi thấy rằng việc tăng kích thước BN không ảnh hưởng đáng kể đến năng lượng hấp phụ của thuốc Iso đối với tất cả các hình dạng hạt nano BN khác nhau. Tất cả các năng lượng hấp phụ thu được bằng các phép tính MC đều là các giá trị âm cho thấy sự hấp phụ của phân tử thuốc Iso trên bề mặt BN là tỏa nhiệt, tự phát và thuận lợi về mặt năng lượng. Ngoài ra, độ ổn định của phức hợp thuốc B12N12-FLN-Iso trong nước đã được nghiên cứu một cách rõ ràng bằng mô phỏng MD. Mô phỏng MD xác nhận rằng các phức hợp iso-B12N12-FLN ổn định khi có mặt các phân tử nước. Vì vậy, cuối cùng, chúng tôi suy ra rằng các nano cụm giống fullerene B12N12 có thể hoạt động như một chất mang thuốc cho thuốc chống lao isoniazid. Được truyền đạt bởi Ramaswamy H. Sarma."} +{"idx": 1870458, "en": "Background: Emerging adulthood (18-25 years) represents a risky time for mental health and substance use. Emerging adults are particularly susceptible to problematic patterns of substance use, especially if they experience anxiety and/or depression and use substances as a way to cope with such issues. However, many mental health treatments do not address substance use. We developed an ecological momentary assessment and intervention (EMA/EMI) to specifically target the motive of drinking to cope with anxiety/depression. Methods: Project CHOICE was a 6-week intervention that paired in-person normative feedback with daily EMA and, if an individual reported negative affect and intent to drink, an EMI was immediately sent to their phone (a personally-chosen coping skill). We recruited n = 20 (55% female, mean age 21.74, 85% Caucasian and 75% non-Hispanic/Latino) individuals from a psychiatric partial hospitalization program for a 6-week open trial of the CHOICE intervention and re-assessed at the 6-week follow-up point. Results: Results indicated that drinking variables and coping motives were highly correlated at baseline. Days of drinking, alcohol-related problems, and coping motives significantly decreased over time following the intervention. Results indicated high levels of feasibility and acceptability. Conclusions: This open pilot represents a feasible, acceptable, and promising direction in delivering interventions in the moment when risk is highest, utilizing smartphone capabilities.", "gemini": "Bối cảnh: Tuổi trưởng thành mới (18-25 tuổi) là thời điểm có nguy cơ đối với sức khỏe tâm thần và sử dụng chất gây nghiện. Những người trưởng thành mới đặc biệt dễ bị các kiểu sử dụng chất gây nghiện có vấn đề, đặc biệt nếu họ bị lo lắng và/hoặc trầm cảm và sử dụng chất gây nghiện như một cách để đối phó với những vấn đề đó. Tuy nhiên, nhiều phương pháp điều trị sức khỏe tâm thần không đề cập đến việc sử dụng chất gây nghiện. Chúng tôi đã phát triển một đánh giá và can thiệp theo thời điểm sinh thái (EMA/EMI) để nhắm mục tiêu cụ thể đến động cơ uống rượu để đối phó với lo lắng/trầm cảm. Phương pháp: Dự án CHOICE là một can thiệp kéo dài 6 tuần kết hợp phản hồi chuẩn mực trực tiếp với EMA hàng ngày và nếu một cá nhân báo cáo ảnh hưởng tiêu cực và ý định uống rượu, một EMI sẽ được gửi ngay đến điện thoại của họ (một kỹ năng đối phó được lựa chọn cá nhân). Chúng tôi đã tuyển dụng n = 20 (55% nữ, tuổi trung bình 21,74, 85% người da trắng và 75% không phải người gốc Tây Ban Nha/Latinh) cá nhân từ một chương trình nhập viện bán thời gian về tâm thần cho một thử nghiệm mở 6 tuần về can thiệp CHOICE và đánh giá lại tại thời điểm theo dõi 6 tuần. Kết quả: Kết quả cho thấy các biến liên quan đến việc uống rượu và động cơ đối phó có mối tương quan cao ở giai đoạn đầu. Số ngày uống rượu, các vấn đề liên quan đến rượu và động cơ đối phó giảm đáng kể theo thời gian sau can thiệp. Kết quả cho thấy mức khả thi và chấp nhận được cao. Kết luận: Dự án thí điểm mở này đại diện cho một hướng đi khả thi, chấp nhận được và đầy hứa hẹn trong việc đưa ra các can thiệp tại thời điểm rủi ro cao nhất, sử dụng các khả năng của điện thoại thông minh."} +{"idx": 1870459, "en": "The term \"empathy\" is popular, yet fuzzy. How laypeople define it has not been investigated. In Study 1, we analyzed 99 participants' free narratives describing their personal definition, and in Study 1 (N = 191) and Study 2 (N = 351), we asked participants to rate a list of specific behaviors and tendencies for how well each one matched their personal definition. Out of 10 coded components, perspective taking was mentioned most often and personal distress (anxious reactivity in emotional situations) was never mentioned. Item ratings revealed four Empathy Concept factors: Prosocial Emotional Response, Interpersonal Perceptiveness, Other Perspective, and Anxious Reactivity. Other Perspective and Prosocial Emotional Response were most highly endorsed while Anxious Reactivity showed the lowest endorsement. Individuals varied widely in their endorsements of the factors. These results demonstrate that laypeople hold a multifactorial set of definitions of empathy and differ widely from one another in which ones they endorse.", "gemini": "Thuật ngữ \"thấu cảm\" rất phổ biến, nhưng lại mơ hồ. Cách người dân thường định nghĩa nó vẫn chưa được điều tra. Trong Nghiên cứu 1, chúng tôi đã phân tích 99 lời kể tự do của những người tham gia mô tả định nghĩa cá nhân của họ, và trong Nghiên cứu 1 (N = 191) và Nghiên cứu 2 (N = 351), chúng tôi đã yêu cầu những người tham gia đánh giá một danh sách các hành vi và xu hướng cụ thể để biết mức độ phù hợp của từng hành vi và xu hướng với định nghĩa cá nhân của họ. Trong số 10 thành phần được mã hóa, việc nắm bắt quan điểm được đề cập nhiều nhất và sự đau khổ cá nhân (phản ứng lo lắng trong các tình huống cảm xúc) không bao giờ được đề cập. Đánh giá các mục đã tiết lộ bốn yếu tố của Khái niệm thấu cảm: Phản ứng cảm xúc xã hội, Nhận thức giữa các cá nhân, Quan điểm khác và Phản ứng lo lắng. Quan điểm khác và Phản ứng cảm xúc xã hội được xác nhận cao nhất trong khi Phản ứng lo lắng cho thấy sự xác nhận thấp nhất. Các cá nhân có sự xác nhận rất khác nhau đối với các yếu tố. Những kết quả này chứng minh rằng người dân thường có một tập hợp các định nghĩa đa yếu tố về sự đồng cảm và khác nhau rất nhiều về những định nghĩa mà họ xác nhận."} +{"idx": 1870460, "en": "Many parts of the plant Nyctanthes arbor-tristis Linn. are widely investigated for their biological properties. Purified Arbortristosides from seeds are reported as anticancer, anti-leishmania, anti-inflammatory, anti-allergic, immunomodulatory and antiviral. The present study elaborates on the isolation, structural and functional characterization of Arbortristoside-C and its inhibition properties against alpha-glucosidase, an important target for diabetes mellitus. Arbortristoside-C is purified from seeds of N. arbor-tristis by extraction using polar fractionation and chromatographic techniques. Arbortristoside-C has been characterized using Ultra Violet (UV), Mass (MS), Infra-Red (IR) and Nuclear Magnetic Resonance (NMR). Inhibition kinetics and Isothermal Titration Calorimetry (ITC) were used for activity and binding characteristics of acarbose and Arbortristoside-C using in-house purified α-glucosidase from Bos taurus. Modeling, docking and structural comparison with acarbose bound structure revealing the similar binding characteristics of Arbortristoside-C which include interaction with catalytic acid/base Aspartic acid residue. Cytotoxicity assay revealed that 100 µg/ml is the maximum toxic-free concentration of Arbortristoside-C. The purified Arbortristoside-C showed inhibition against mammalian α-glucosidase, suggesting its potential to treat Diabetes mellitus.", "gemini": "Nhiều bộ phận của cây Nyctanthes arbor-tristis Linn. được nghiên cứu rộng rãi về các đặc tính sinh học của chúng. Arbortristosides tinh khiết từ hạt được báo cáo có tác dụng chống ung thư, chống leishmania, chống viêm, chống dị ứng, điều hòa miễn dịch và chống vi-rút. Nghiên cứu hiện tại trình bày chi tiết về việc phân lập, đặc tính cấu trúc và chức năng của Arbortristoside-C và các đặc tính ức chế của nó đối với alpha-glucosidase, một mục tiêu quan trọng đối với bệnh tiểu đường. Arbortristoside-C được tinh chế từ hạt của N. arbor-tristis bằng phương pháp chiết xuất sử dụng phân đoạn phân cực và kỹ thuật sắc ký. Arbortristoside-C đã được đặc trưng bằng tia cực tím (UV), khối lượng (MS), hồng ngoại (IR) và cộng hưởng từ hạt nhân (NMR). Động học ức chế và nhiệt lượng kế chuẩn độ đẳng nhiệt (ITC) được sử dụng để hoạt động và đặc tính liên kết của acarbose và Arbortristoside-C sử dụng α-glucosidase tinh khiết tại nhà từ Bos taurus. Mô hình hóa, gắn kết và so sánh cấu trúc với cấu trúc liên kết acarbose cho thấy các đặc tính liên kết tương tự của Arbortristoside-C bao gồm tương tác với axit/bazơ xúc tác là axit aspartic. Thử nghiệm độc tính tế bào cho thấy 100 µg/ml là nồng độ không độc tối đa của Arbortristoside-C. Arbortristoside-C tinh khiết cho thấy khả năng ức chế α-glucosidase ở động vật có vú, cho thấy tiềm năng điều trị bệnh tiểu đường của nó."} +{"idx": 1870461, "en": "Donor-conceived neonates have poorer birth outcomes, including low birth weight and preterm delivery that are associated with poorer long-term health in adulthood through the developmental origins of health and disease (DOHaD) theory. The aim of this study was to conduct the first investigation of the adult health outcomes of donor-conceived people. An online health survey was completed by 272 donor sperm-conceived adults and 877 spontaneously conceived adults from around the world. Donor and spontaneously conceived groups were matched for age, sex, height, smoking, alcohol consumption, exercise, own fertility and maternal smoking. Donor sperm-conceived adults had significantly higher reports of being diagnosed with type 1 diabetes (P = 0.031), thyroid disease (P = 0.031), acute bronchitis (P = 0.008), environmental allergies (P = 0.046), sleep apnoea (P = 0.037) and having ear tubes/grommets surgically implanted (P = 0.046). This is the first study to investigate the health outcomes of adult donor sperm-conceived people. Donor sperm-conceived adults self-reported elevated frequencies of various health conditions. The outcomes are consistent with birth defect data from donor sperm treatment and are consistent with the DOHaD linking perturbed early growth and chronic disease in adulthood.", "gemini": "Trẻ sơ sinh được thụ thai từ người hiến tặng có kết quả sinh kém hơn, bao gồm cân nặng khi sinh thấp và sinh non, những yếu tố này liên quan đến sức khỏe kém hơn về lâu dài ở tuổi trưởng thành thông qua lý thuyết về nguồn gốc phát triển của bệnh và sức khỏe (DOHaD). Mục đích của nghiên cứu này là tiến hành điều tra đầu tiên về kết quả sức khỏe của người lớn được thụ thai từ người hiến tặng. Một cuộc khảo sát sức khỏe trực tuyến đã được thực hiện bởi 272 người lớn được thụ thai từ tinh trùng hiến tặng và 877 người lớn được thụ thai tự nhiên từ khắp nơi trên thế giới. Các nhóm thụ thai từ người hiến tặng và thụ thai tự nhiên được ghép đôi theo tuổi, giới tính, chiều cao, hút thuốc, uống rượu, tập thể dục, khả năng sinh sản của chính họ và hút thuốc của mẹ. Người lớn được thụ thai từ tinh trùng hiến tặng có báo cáo cao hơn đáng kể về việc được chẩn đoán mắc bệnh tiểu đường loại 1 (P = 0,031), bệnh tuyến giáp (P = 0,031), viêm phế quản cấp (P = 0,008), dị ứng môi trường (P = 0,046), ngưng thở khi ngủ (P = 0,037) và đã phẫu thuật cấy ống tai/nút tai (P = 0,046). Đây là nghiên cứu đầu tiên điều tra kết quả sức khỏe của những người trưởng thành được thụ thai từ tinh trùng hiến tặng. Người lớn được thụ thai từ tinh trùng hiến tặng tự báo cáo tần suất gia tăng của nhiều tình trạng sức khỏe khác nhau. Các kết quả phù hợp với dữ liệu về dị tật bẩm sinh từ phương pháp điều trị tinh trùng hiến tặng và phù hợp với DOHaD liên kết sự tăng trưởng sớm bị nhiễu loạn và bệnh mãn tính ở tuổi trưởng thành."} +{"idx": 1870462, "en": "Pulmonary MRI provides structural and quantitative functional images of the lungs without ionizing radiation, but it has had limited clinical use due to low signal intensity from the lung parenchyma. The lack of radiation makes pulmonary MRI an ideal modality for pediatric examinations, pregnant women, and patients requiring serial and longitudinal follow-up. Fortunately, recent MRI techniques, including ultrashort echo time and zero echo time, are expanding clinical opportunities for pulmonary MRI. With the use of multicoil parallel acquisitions and acceleration methods, these techniques make pulmonary MRI practical for evaluating lung parenchymal and pulmonary vascular diseases. The purpose of this Fleischner Society position paper is to familiarize radiologists and other interested clinicians with these advances in pulmonary MRI and to stratify the Society recommendations for the clinical use of pulmonary MRI into three categories: (a) suggested for current clinical use, (b) promising but requiring further validation or regulatory approval, and (c) appropriate for research investigations. This position paper also provides recommendations for vendors and infrastructure, identifies methods for hypothesis-driven research, and suggests opportunities for prospective, randomized multicenter trials to investigate and validate lung MRI methods.", "gemini": "MRI phổi cung cấp hình ảnh cấu trúc và chức năng định lượng của phổi mà không cần bức xạ ion hóa, nhưng nó có hạn chế trong sử dụng lâm sàng do cường độ tín hiệu thấp từ nhu mô phổi. Việc không có bức xạ làm cho MRI phổi trở thành phương thức lý tưởng để kiểm tra nhi khoa, phụ nữ mang thai và những bệnh nhân cần theo dõi theo dõi theo chuỗi và theo chiều dọc. May mắn thay, các kỹ thuật MRI gần đây, bao gồm thời gian hồi âm cực ngắn và thời gian hồi âm bằng không, đang mở rộng các cơ hội lâm sàng cho MRI phổi. Với việc sử dụng các phương pháp thu nhận song song đa cuộn dây và phương pháp tăng tốc, các kỹ thuật này giúp MRI phổi trở nên thiết thực để đánh giá các bệnh về nhu mô phổi và mạch máu phổi. Mục đích của bài báo lập trường của Hiệp hội Fleischner này là làm quen với các bác sĩ X quang và các bác sĩ lâm sàng quan tâm khác về những tiến bộ này trong MRI phổi và phân tầng các khuyến nghị của Hiệp hội về việc sử dụng MRI phổi trong lâm sàng thành ba loại: (a) đề xuất sử dụng lâm sàng hiện tại, (b) hứa hẹn nhưng cần xác nhận thêm hoặc phê duyệt theo quy định và (c) phù hợp cho các cuộc điều tra nghiên cứu. Bài báo lập trường này cũng cung cấp các khuyến nghị cho các nhà cung cấp và cơ sở hạ tầng, xác định các phương pháp nghiên cứu dựa trên giả thuyết và đề xuất các cơ hội cho các thử nghiệm đa trung tâm ngẫu nhiên có triển vọng để điều tra và xác nhận các phương pháp MRI phổi."} +{"idx": 1870463, "en": "STUDY OBJECTIVES Various lines of clinical findings have suggested abnormalities in macro- or microstructural parameters of sleep in patients with schizophrenia. Meanwhile findings are inconclusive due to some confounding factors, such as the heterogeneity of the disorder, drug regimen, and duration of the illness. There are a few studies in the literature that have been conducted on drug-free patients with first-episode psychosis (FEP). Based on this knowledge, we aimed to explore sleep characteristics, sleep spindles, and neuropsychological profiles of the drug-naive patients with FEP. METHODS The study sample consisted of 21 drug-naive patients with FEP and 21 healthy participants. Polysomnography recordings were conducted for 2 subsequent nights. A neuropsychological test battery was administered for assessing cognitive functions. The Positive and Negative Syndrome Scale was applied to measure symptom severity of the patients. Spindle detection was performed visually. RESULTS According to the results of the study, the patient group's percentage of stage N2 sleep and sleep efficiency index was lower than in the control group. Among sleep spindle parameters, spindle density was found to be reduced in the patient group. The results of neuropsychological tests measuring executive functions, learning, and memory support the idea that there is a global cognitive deterioration from the early course of the disorder. In the psychotic group, negative symptoms were negatively correlated with verbal memory, learning, verbal fluency, and semantic organization. We found that the percentage of stage N3 sleep decreased while negative symptom severity increased. In addition, the percentage of stage N1 sleep increased as negative symptom severity increased. Reduction in stage N3 sleep was associated with an impairment in learning, verbal fluency, and response inhibition. The sleep spindle density and cognitive functions did not show any associations. CONCLUSIONS Taken together, these findings suggest that patients with FEP show global cognitive impairment (except for attention and processing speed), which is associated with changes in sleep architecture and higher score in a scale assessing negative symptoms. We conclude that cognitive function and spindle parameters differ nonlinearly among patients with FEP.", "gemini": "MỤC TIÊU NGHIÊN CỨU Nhiều kết quả lâm sàng khác nhau đã gợi ý những bất thường về các thông số vĩ mô hoặc vi mô của giấc ngủ ở những bệnh nhân mắc chứng tâm thần phân liệt. Trong khi đó, các phát hiện vẫn chưa có kết luận do một số yếu tố gây nhiễu, chẳng hạn như tính không đồng nhất của rối loạn, phác đồ dùng thuốc và thời gian mắc bệnh. Có một vài nghiên cứu trong tài liệu đã được tiến hành trên những bệnh nhân không dùng thuốc bị loạn thần lần đầu (FEP). Dựa trên kiến thức này, chúng tôi đã đặt mục tiêu khám phá các đặc điểm của giấc ngủ, trục xoay giấc ngủ và hồ sơ tâm lý thần kinh của những bệnh nhân chưa dùng thuốc bị FEP. PHƯƠNG PHÁP Mẫu nghiên cứu bao gồm 21 bệnh nhân chưa dùng thuốc bị FEP và 21 người tham gia khỏe mạnh. Ghi điện não đồ ngủ được thực hiện trong 2 đêm liên tiếp. Một bộ pin kiểm tra tâm lý thần kinh đã được sử dụng để đánh giá các chức năng nhận thức. Thang đo hội chứng dương tính và tiêu cực được áp dụng để đo mức độ nghiêm trọng của các triệu chứng của bệnh nhân. Phát hiện trục xoay được thực hiện trực quan. KẾT QUẢ Theo kết quả của nghiên cứu, tỷ lệ phần trăm giấc ngủ giai đoạn N2 và chỉ số hiệu quả giấc ngủ của nhóm bệnh nhân thấp hơn so với nhóm đối chứng. Trong số các thông số trục xoay giấc ngủ, mật độ trục xoay được phát hiện là giảm ở nhóm bệnh nhân. Kết quả của các bài kiểm tra tâm lý thần kinh đo các chức năng điều hành, khả năng học tập và trí nhớ hỗ trợ cho ý tưởng rằng có sự suy giảm nhận thức toàn cầu từ giai đoạn đầu của rối loạn. Trong nhóm loạn thần, các triệu chứng tiêu cực có mối tương quan tiêu cực với trí nhớ ngôn ngữ, khả năng học tập, khả năng nói trôi chảy và tổ chức ngữ nghĩa. Chúng tôi thấy rằng tỷ lệ phần trăm giấc ngủ giai đoạn N3 giảm trong khi mức độ nghiêm trọng của triệu chứng tiêu cực tăng lên. Ngoài ra, tỷ lệ phần trăm giấc ngủ giai đoạn N1 tăng khi mức độ nghiêm trọng của triệu chứng tiêu cực tăng lên. Giảm giấc ngủ giai đoạn N3 có liên quan đến sự suy giảm khả năng học tập, nói trôi chảy và ức chế phản ứng. Mật độ trục xoay giấc ngủ và chức năng nhận thức không cho thấy bất kỳ mối liên hệ nào. KẾT LUẬN Tổng hợp lại, những phát hiện này cho thấy rằng những bệnh nhân bị FEP cho thấy sự suy giảm nhận thức toàn cầu (trừ sự chú ý và tốc độ xử lý), có liên quan đến những thay đổi trong cấu trúc giấc ngủ và điểm số cao hơn trong thang đánh giá các triệu chứng tiêu cực. Chúng tôi kết luận rằng chức năng nhận thức và các thông số trục xoay khác nhau không tuyến tính ở những bệnh nhân bị FEP."} +{"idx": 1870464, "en": "BACKGROUND Contrast-induced nephropathy is a well-recognized acute complication in cancer patients, but the long-term effects of repeated contrast exposure are not known. We analyzed the association of the number of contrast-enhanced computed tomography (CECT) examinations and other clinical factors with decline in estimated glomerular filtration rate (eGFR) in colorectal cancer survivors. MATERIALS AND METHODS We retrospectively queried a prospective surgical colorectal cancer database to identify patients with stage I or II cancer who underwent resection in 2007 - 2013 and were alive for at least 3 years. eGFR was calculated before and 3 years after the surgery with ≥ 20% decline relative to baseline defined as significant and used as the primary outcome. The association of clinical factors with the primary outcome was analyzed using logistic regression. RESULTS Only 256 patients with the median follow-up of 65 months had sufficient clinical data for analysis. Median eGFR decline at follow-up was 3.0 mL/min/1.73m2 or 4% change from baseline. 47 patients (18%) had ≥ 20% reduction in eGFR, which was not associated with the number of CECT examinations. Multivariable analysis demonstrated that increasing age (OR, 1.03; 95% CI, 1.00 - 1.06), presence of diabetes (OR, 2.33; 95% CI, 1.18 - 4.61), and longer operation time (OR, 1.04; 95% CI, 1.01 - 1.07) were independently associated with a higher likelihood of ≥ 20% eGFR decline at 3 years. CONCLUSION Older age, diabetes, and longer operating time, but not cumulative contrast exposure were found to be associated with worse long-term renal outcomes following surgical resection in patients with early-stage colorectal cancer who survived 3 years.", "gemini": "BỐI CẢNH Bệnh thận do thuốc cản quang là một biến chứng cấp tính được biết rõ ở bệnh nhân ung thư, nhưng các tác động lâu dài của việc tiếp xúc với thuốc cản quang nhiều lần vẫn chưa được biết. Chúng tôi đã phân tích mối liên quan giữa số lần chụp cắt lớp vi tính có sử dụng thuốc cản quang (CECT) và các yếu tố lâm sàng khác với sự suy giảm tốc độ lọc cầu thận ước tính (eGFR) ở những người sống sót sau ung thư đại trực tràng. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi đã truy vấn hồi cứu một cơ sở dữ liệu ung thư đại trực tràng phẫu thuật có triển vọng để xác định những bệnh nhân mắc ung thư giai đoạn I hoặc II đã được cắt bỏ vào năm 2007 - 2013 và còn sống ít nhất 3 năm. eGFR được tính trước và 3 năm sau phẫu thuật với ≥ 20% giảm so với giá trị cơ bản được định nghĩa là có ý nghĩa và được sử dụng làm kết quả chính. Mối liên quan của các yếu tố lâm sàng với kết quả chính đã được phân tích bằng hồi quy logistic. KẾT QUẢ Chỉ có 256 bệnh nhân có thời gian theo dõi trung bình là 65 tháng có đủ dữ liệu lâm sàng để phân tích. Độ sụt giảm eGFR trung bình khi theo dõi là 3,0 mL/phút/1,73m2 hoặc thay đổi 4% so với giá trị cơ bản. 47 bệnh nhân (18%) có giảm ≥ 20% eGFR, không liên quan đến số lần chụp CECT. Phân tích đa biến chứng minh rằng tuổi tác tăng (OR, 1,03; CI 95%, 1,00 - 1,06), có bệnh tiểu đường (OR, 2,33; CI 95%, 1,18 - 4,61) và thời gian phẫu thuật dài hơn (OR, 1,04; CI 95%, 1,01 - 1,07) có liên quan độc lập với khả năng suy giảm eGFR ≥ 20% ở 3 năm. KẾT LUẬN Tuổi cao, bệnh tiểu đường và thời gian phẫu thuật dài hơn, nhưng không phải là phơi nhiễm thuốc cản quang tích lũy được phát hiện có liên quan đến kết quả về thận lâu dài tệ hơn sau cắt bỏ phẫu thuật ở những bệnh nhân ung thư đại trực tràng giai đoạn đầu sống sót sau 3 năm."} +{"idx": 1870465, "en": "INTRODUCTION The implantation of acute or chronic vascular accesses for hemodialysis (HD) in end-stage kidney disease patients is a critical skill procedure for nephrologists, with an impact on short- and long-term outcomes of the modality and patient survival. Placement circumstances, however, may depend on the availability of technological support and will likely vary across the world. MATERIALS AND METHODS We retrospectively reviewed our local experience with ultrasound-guided tunneled dialysis catheter (TDC) insertions but without access to fluoroscopic guidance. Data were available for 63 patients with TDCs placed by faculty nephrologists at the dialysis unit procedure rooms between March 2015 and February 2018. We reviewed circumstances of TDC placement, patient characteristics, and procedural outcomes. RESULTS The mean age was 62 (± 41) years, and 46% of the patients were male. All TDC placements were technically successful and no major complications occurred. Most TDCs (52.8%) were a de novo placement. In the de novo patient group, there were 27 right-sided internal jugular vein (IJV) and 6 left-sided IJV cannulations. Blood pump flow was 284.6 (± 58) mL/min via the temporary catheter 1 month before and 316.7 (± 46) mL/min 1 month after TDC placement (p < 0.0001). The majority of catheter tips (63%) reached the right atrial placement position successfully. DISCUSSION Technologically successful TDC placement can be performed without fluoroscopic tip guidance and result in improved access flows and dialysis efficacy when compared to temporary hemodialysis catheters.", "gemini": "GIỚI THIỆU Việc cấy ghép các đường vào mạch cấp tính hoặc mạn tính để lọc máu (HD) ở những bệnh nhân mắc bệnh thận giai đoạn cuối là một thủ thuật kỹ thuật quan trọng đối với các bác sĩ chuyên khoa thận, có tác động đến kết quả của phương thức điều trị và sự sống còn của bệnh nhân trong thời gian ngắn và dài hạn. Tuy nhiên, các trường hợp đặt đường vào mạch có thể phụ thuộc vào khả năng hỗ trợ về mặt công nghệ và có khả năng thay đổi ở khắp nơi trên thế giới. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi đã xem xét lại kinh nghiệm thực tế tại địa phương của mình về việc đặt ống thông lọc máu qua đường hầm (TDC) có hướng dẫn bằng siêu âm nhưng không có hướng dẫn bằng máy chụp X-quang huỳnh quang. Có dữ liệu của 63 bệnh nhân được các bác sĩ chuyên khoa thận đặt TDC tại các phòng thủ thuật của đơn vị lọc máu từ tháng 3 năm 2015 đến tháng 2 năm 2018. Chúng tôi đã xem xét các trường hợp đặt TDC, đặc điểm của bệnh nhân và kết quả thủ thuật. KẾT QUẢ Độ tuổi trung bình là 62 (± 41) tuổi và 46% bệnh nhân là nam. Tất cả các trường hợp đặt TDC đều thành công về mặt kỹ thuật và không xảy ra biến chứng lớn nào. Hầu hết các TDC (52,8%) đều được đặt lần đầu. Trong nhóm bệnh nhân đặt lần đầu, có 27 ca đặt ống thông tĩnh mạch cảnh trong (IJV) bên phải và 6 ca đặt ống thông IJV bên trái. Lưu lượng bơm máu là 284,6 (± 58) mL/phút qua ống thông tạm thời 1 tháng trước và 316,7 (± 46) mL/phút 1 tháng sau khi đặt TDC (p < 0,0001). Hầu hết các đầu ống thông (63%) đều đạt được vị trí đặt tại tâm nhĩ phải một cách thành công. THẢO LUẬN Việc đặt TDC thành công về mặt kỹ thuật có thể được thực hiện mà không cần hướng dẫn bằng máy chụp X-quang huỳnh quang và dẫn đến cải thiện lưu lượng vào mạch và hiệu quả lọc máu khi so sánh với ống thông lọc máu tạm thời."} +{"idx": 1870466, "en": "C3 glomerulonephritis (C3GN) is a rare but severe form of kidney disease caused by fluid-phase dysregulation of the alternative complement pathway. Causative mutations in complement regulating genes as well as auto-immune forms of C3GN have been described. However, therapy and prognosis in individual patients remain a matter of debate and long-term data are scarce. This also applies for the management of transplant patients as disease recurrence post-transplant is frequent. Here, we depict the clinical courses of two sisters with the unique combination of an identical, homozygous mutation in the complement factor H (CFH) gene as well as autoantibodies with a clinical follow-up of more than 20 years. Interestingly, the sisters presented with discordant clinical courses of C3GN with normal kidney function in one (patient A) and end-stage kidney disease in the other sister (patient B). In patient B, eculizumab was administered immediately prior to and in the course after kidney transplantation, with the result of a stable graft function without any signs of disease recurrence. Comprehensive genetic work-up revealed no further disease-causing mutation in both sisters. Intriguingly, the auto-antibody profile substantially differed in both sisters: autoantibodies in patient A reduced the C3b deposition, while the antibodies identified in patient B increased complement activation and deposition of split products. This study underlines the concept of a personalized-medicine approach in complement-associated diseases after thorough evaluation of the individual risk profile in each patient.", "gemini": "Viêm cầu thận C3 (C3GN) là một dạng bệnh thận hiếm gặp nhưng nghiêm trọng do rối loạn điều hòa pha lỏng của con đường bổ sung thay thế. Người ta đã mô tả các đột biến gây bệnh ở các gen điều hòa bổ sung cũng như các dạng tự miễn của C3GN. Tuy nhiên, liệu pháp điều trị và tiên lượng ở từng bệnh nhân vẫn là vấn đề gây tranh cãi và dữ liệu về lâu dài còn rất ít. Điều này cũng áp dụng cho việc quản lý bệnh nhân ghép tạng vì bệnh thường tái phát sau ghép. Ở đây, chúng tôi mô tả diễn biến lâm sàng của hai chị em gái có sự kết hợp độc đáo giữa đột biến đồng hợp tử giống hệt nhau ở gen yếu tố bổ sung H (CFH) cũng như tự kháng thể với thời gian theo dõi lâm sàng hơn 20 năm. Thật thú vị, hai chị em có diễn biến lâm sàng không nhất quán của C3GN với chức năng thận bình thường ở một người (bệnh nhân A) và bệnh thận giai đoạn cuối ở người chị em kia (bệnh nhân B). Ở bệnh nhân B, eculizumab đã được dùng ngay trước và trong quá trình sau khi ghép thận, với kết quả là chức năng ghép ổn định mà không có bất kỳ dấu hiệu tái phát bệnh nào. Việc kiểm tra di truyền toàn diện cho thấy không có đột biến gây bệnh nào khác ở cả hai chị em. Thật thú vị, hồ sơ tự kháng thể khác nhau đáng kể ở cả hai chị em: tự kháng thể ở bệnh nhân A làm giảm lắng đọng C3b, trong khi kháng thể được xác định ở bệnh nhân B làm tăng hoạt hóa bổ sung và lắng đọng các sản phẩm phân chia. Nghiên cứu này nhấn mạnh khái niệm về phương pháp tiếp cận y học cá nhân hóa trong các bệnh liên quan đến bổ sung sau khi đánh giá kỹ lưỡng hồ sơ nguy cơ của từng bệnh nhân."} +{"idx": 1870467, "en": "Amyloid light-chain (AL) amyloidosis represents the most common type of amyloid affecting the kidneys. As AL amyloidosis is frequently clinically manifested as nephrotic syndrome, this glomerular syndrome has been improperly linked to all other types of kidney amyloidosis. In this report, we highlight the importance of amyloid typing, as the deposition of several amyloidotic proteins in the kidneys is not associated with heavy proteinuria. We present two cases of patients who presented with sudden-onset nephrotic syndrome and kidney biopsies showing interstitial, vascular, and/or mesangial LECT2 amyloidosis. Further examination by electron microscopy demonstrated diffuse foot process effacement consistent with minimal change disease and no amyloid deposition in the glomerular basement membrane. Both patients had complete remission after glucocorticoid treatment. We conclude that the presence of nephrotic syndrome in a patient with LECT2 amyloidosis must alert for a potential concurrent podocytopathy.", "gemini": "Bệnh lắng đọng chuỗi nhẹ amyloid (AL) là loại amyloid phổ biến nhất ảnh hưởng đến thận. Vì bệnh lắng đọng amyloid AL thường biểu hiện lâm sàng là hội chứng thận hư, nên hội chứng cầu thận này đã được liên kết không đúng cách với tất cả các loại bệnh lắng đọng amyloid ở thận khác. Trong báo cáo này, chúng tôi nhấn mạnh tầm quan trọng của việc phân loại amyloid, vì sự lắng đọng của một số protein amyloid trong thận không liên quan đến tình trạng protein niệu nặng. Chúng tôi trình bày hai trường hợp bệnh nhân bị hội chứng thận hư khởi phát đột ngột và sinh thiết thận cho thấy bệnh lắng đọng amyloid LECT2 ở mô kẽ, mạch máu và/hoặc màng đáy cầu thận. Kiểm tra thêm bằng kính hiển vi điện tử cho thấy sự mất chân gai lan tỏa phù hợp với bệnh thay đổi tối thiểu và không có lắng đọng amyloid ở màng đáy cầu thận. Cả hai bệnh nhân đều thuyên giảm hoàn toàn sau khi điều trị bằng glucocorticoid. Chúng tôi kết luận rằng sự hiện diện của hội chứng thận hư ở bệnh nhân bị bệnh lắng đọng amyloid LECT2 phải cảnh báo về khả năng mắc bệnh bội nhiễm ở tế bào biểu mô cầu thận."} +{"idx": 1870468, "en": "Methylation profiling has become a mainstay in brain tumor diagnostics since the introduction of the first publicly available classification tool by the German Cancer Research Center in 2017. We demonstrate the capability of this system through an example of a rare case of IDH wildtype glioblastoma diagnosed in a patient previously treated for T-cell acute lymphoblastic leukemia. Our novel in-house diagnostic tool EpiDiP provided hints arguing against a radiation-induced tumor, identified a novel recurrent genetic aberration, and thus informed about a potential therapeutic target.", "gemini": "Phân tích cấu hình metyl hóa đã trở thành trụ cột trong chẩn đoán u não kể từ khi Trung tâm nghiên cứu ung thư Đức giới thiệu công cụ phân loại công khai đầu tiên vào năm 2017. Chúng tôi chứng minh khả năng của hệ thống này thông qua ví dụ về một trường hợp hiếm gặp của bệnh u nguyên bào thần kinh đệm IDH kiểu hoang dã được chẩn đoán ở một bệnh nhân trước đây được điều trị bệnh bạch cầu lymphoblastic cấp tế bào T. Công cụ chẩn đoán nội bộ mới EpiDiP của chúng tôi cung cấp những gợi ý phản bác lại khối u do bức xạ gây ra, xác định một quang sai di truyền tái phát mới và do đó cung cấp thông tin về một mục tiêu điều trị tiềm năng."} +{"idx": 1870469, "en": "OBJECTIVE This paper analyzes the potential outliers in the bioanalytical and clinical part of a bioequivalence study, the effect on bioequivalence decisions whether or not it is appropriate to eliminate them from the statistical evaluation of bioequivalence. MATERIALS AND METHODS The clinical part was a cross-over, two periods, two sequences bioequivalence study concerning two piroxicam formulations, on healthy subjects. A simulation study evaluated the influence of 10% errors on the percent bias of calculated concentrations from nominal ones. RESULTS In bioequivalence studies, it is not possible to distinguish between relevant types of outliers based only on statistical criteria. The \"problem\" is particularly acute when the omission of outliers leads to a bias in the decision concerning bioequivalence from rejection to acceptance. In such cases, there is the suspicion of subjective analysis and torture of data. The effect of analytical errors at high plasma levels was criticized for the calculated concentrations in the neighborhood of lower limit of quantification. Errors at low concentrations have a less significant effect. In the pharmacokinetic analysis, several types of outliers were shown: single points, curves, pairs of curves corresponding to the same subject, intrasubject ratios of areas under curves and maximum concentrations. These pharmacokinetic outliers could have had, at the same time, bioanalytical, physiological and physicochemical causes. CONCLUSION Considering the results, it was proposed the following algorithm in the analysis of outlier data and outlier subjects in bioequivalence studies: evaluation of the implications of the decision concerning elimination of outliers on the decision concerning bioequivalence; application of the statistic tests for detection of outliers data; evaluations from the point of view of physiological pharmacokinetics, final decision concerning elimination of outliers.", "gemini": "MỤC TIÊU Bài báo này phân tích các giá trị ngoại lệ tiềm ẩn trong phần sinh học phân tích và lâm sàng của một nghiên cứu tương đương sinh học, tác động đến các quyết định về tương đương sinh học cho dù có nên loại bỏ chúng khỏi đánh giá thống kê về tương đương sinh học hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Phần lâm sàng là nghiên cứu tương đương sinh học chéo, hai giai đoạn, hai trình tự liên quan đến hai chế phẩm piroxicam trên đối tượng khỏe mạnh. Một nghiên cứu mô phỏng đã đánh giá ảnh hưởng của sai số 10% đối với độ lệch phần trăm của nồng độ được tính toán so với nồng độ danh nghĩa. KẾT QUẢ Trong các nghiên cứu tương đương sinh học, không thể phân biệt giữa các loại giá trị ngoại lệ có liên quan chỉ dựa trên các tiêu chuẩn thống kê. \"Vấn đề\" đặc biệt nghiêm trọng khi việc bỏ sót các giá trị ngoại lệ dẫn đến sai lệch trong quyết định về tương đương sinh học từ việc từ chối sang chấp nhận. Trong những trường hợp như vậy, có nghi ngờ về phân tích chủ quan và tra tấn dữ liệu. Tác động của các lỗi phân tích ở nồng độ huyết tương cao đã bị chỉ trích đối với các nồng độ được tính toán trong vùng lân cận của giới hạn định lượng thấp hơn. Sai số ở nồng độ thấp có tác động ít đáng kể hơn. Trong phân tích dược động học, một số loại giá trị ngoại lệ đã được chỉ ra: các điểm đơn, các đường cong, các cặp đường cong tương ứng với cùng một đối tượng, tỷ số diện tích dưới đường cong trong cùng một đối tượng và nồng độ tối đa. Các giá trị ngoại lệ dược động học này có thể có cùng lúc các nguyên nhân sinh học phân tích, sinh lý và lý hóa. KẾT LUẬN Xét đến các kết quả, thuật toán sau đây đã được đề xuất trong phân tích dữ liệu ngoại lệ và đối tượng ngoại lệ trong các nghiên cứu tương đương sinh học: đánh giá các tác động của quyết định về việc loại bỏ các giá trị ngoại lệ đối với quyết định về tương đương sinh học; áp dụng các bài kiểm tra thống kê để phát hiện dữ liệu ngoại lệ; đánh giá từ quan điểm dược động học sinh lý, quyết định cuối cùng về việc loại bỏ các giá trị ngoại lệ."} +{"idx": 1870470, "en": "INTRODUCTION Femoral shaft fractures in pediatric patients are treated by elastic intramedullary nailing using titanium or stainless-steel nails. The elastic stable intramedullary nailing behaves as an internal splint, promoting early mobilization. This type of treatment involves a minimally invasive approach, no damage to the growth plates, and no impairment of femoral head blood supply. PURPOSE The aim of our study was to identify the negative predicting factors that might lead to an increased complication rate after elastic stable intramedullary nailing of femoral shaft fractures in children. METHODS We conducted a retrospective study on 137 patients with femoral shaft fractures treated by elastic stable intramedullary nailing. Patients' age ranged between 4 and 17 years. We used data from the medical records of the patients to evaluate postoperative complications. Plain radiographs were analyzed to determine the fracture type, fracture location, and postoperative complications such as delayed union, angular deformities, and limb length discrepancies. Multivariate analysis was conducted to identify predictors for poor outcomes. RESULTS Complications occurred in 29 patients (21%) and consisted of delayed union, axial deformities, or lower limb length discrepancies. In the group of patients that suffered from complications, mechanism of injury, age, and weight were significant. They were older by an average of 5 years; half of them weighed more than 50 kg and over a half were involved in a road traffic accident. CONCLUSIONS Elastic nailing is a successful tool to treat femoral shaft fractures. Three factors were demonstrated to influence the outcome. The mechanism of injury, age > 11 years, and weight > 50 kg are the most important and are predictors for development of complications such as delayed union or deformity.", "gemini": "GIỚI THIỆU Gãy thân xương đùi ở bệnh nhi được điều trị bằng đóng đinh nội tủy đàn hồi bằng đinh titan hoặc thép không gỉ. Đóng đinh nội tủy ổn định đàn hồi hoạt động như một nẹp cố định bên trong, thúc đẩy vận động sớm. Loại điều trị này bao gồm phương pháp tiếp cận ít xâm lấn, không làm hỏng đĩa tăng trưởng và không làm suy giảm nguồn cung cấp máu cho đầu xương đùi. MỤC ĐÍCH Mục đích nghiên cứu của chúng tôi là xác định các yếu tố dự đoán tiêu cực có thể dẫn đến tăng tỷ lệ biến chứng sau khi đóng đinh nội tủy ổn định đàn hồi trong gãy thân xương đùi ở trẻ em. PHƯƠNG PHÁP Chúng tôi đã tiến hành một nghiên cứu hồi cứu trên 137 bệnh nhân bị gãy thân xương đùi được điều trị bằng đóng đinh nội tủy ổn định đàn hồi. Độ tuổi của bệnh nhân từ 4 đến 17 tuổi. Chúng tôi sử dụng dữ liệu từ hồ sơ bệnh án của bệnh nhân để đánh giá các biến chứng sau phẫu thuật. Các phim X quang thông thường được phân tích để xác định loại gãy, vị trí gãy và các biến chứng sau phẫu thuật như liền xương chậm, biến dạng góc và chênh lệch chiều dài chi. Phân tích đa biến được tiến hành để xác định các yếu tố dự đoán kết quả kém. KẾT QUẢ Biến chứng xảy ra ở 29 bệnh nhân (21%) và bao gồm liền xương chậm, biến dạng trục hoặc chênh lệch chiều dài chi dưới. Trong nhóm bệnh nhân bị biến chứng, cơ chế chấn thương, tuổi và cân nặng có ý nghĩa. Họ lớn hơn trung bình 5 tuổi; một nửa trong số họ nặng hơn 50 kg và hơn một nửa liên quan đến tai nạn giao thông. KẾT LUẬN Đóng đinh đàn hồi là một công cụ thành công để điều trị gãy thân xương đùi. Ba yếu tố đã được chứng minh là ảnh hưởng đến kết quả. Cơ chế chấn thương, tuổi> 11 và cân nặng> 50 kg là những yếu tố quan trọng nhất và là yếu tố dự đoán sự phát triển của các biến chứng như liền xương chậm hoặc biến dạng."} +{"idx": 1870471, "en": "Vitamin K epoxide reductase (VKOR) drives the vitamin K cycle, activating vitamin K-dependent blood clotting factors. VKOR is also the target of the widely used anticoagulant drug, warfarin. Despite VKOR's pivotal role in coagulation, its structure and active site remain poorly understood. In addition, VKOR variants can cause vitamin K-dependent clotting factor deficiency or alter warfarin response. Here, we used multiplexed, sequencing-based assays to measure the effects of 2,695 VKOR missense variants on abundance and 697 variants on activity in cultured human cells. The large-scale functional data, along with an evolutionary coupling analysis, supports a four transmembrane domain topology, with variants in transmembrane domains exhibiting strongly deleterious effects on abundance and activity. Functionally constrained regions of the protein define the active site, and we find that, of four conserved cysteines putatively critical for function, only three are absolutely required. Finally, 25% of human VKOR missense variants show reduced abundance or activity, possibly conferring warfarin sensitivity or causing disease.", "gemini": "Vitamin K epoxide reductase (VKOR) thúc đẩy chu trình vitamin K, kích hoạt các yếu tố đông máu phụ thuộc vitamin K. VKOR cũng là mục tiêu của thuốc chống đông máu được sử dụng rộng rãi, warfarin. Mặc dù VKOR có vai trò quan trọng trong quá trình đông máu, cấu trúc và vị trí hoạt động của nó vẫn chưa được hiểu rõ. Ngoài ra, các biến thể VKOR có thể gây ra tình trạng thiếu yếu tố đông máu phụ thuộc vitamin K hoặc làm thay đổi phản ứng với warfarin. Ở đây, chúng tôi đã sử dụng các xét nghiệm dựa trên giải trình tự đa kênh để đo lường tác động của 2.695 biến thể mất nghĩa VKOR đối với sự phong phú và 697 biến thể đối với hoạt động trong các tế bào người nuôi cấy. Dữ liệu chức năng quy mô lớn, cùng với phân tích ghép nối tiến hóa, hỗ trợ cấu trúc miền xuyên màng bốn, với các biến thể trong miền xuyên màng thể hiện các tác động có hại mạnh mẽ đến sự phong phú và hoạt động. Các vùng bị hạn chế chức năng của protein xác định vị trí hoạt động và chúng tôi thấy rằng, trong số bốn cystein bảo tồn được cho là rất quan trọng đối với chức năng, chỉ có ba cystein là hoàn toàn cần thiết. Cuối cùng, 25% các biến thể mất nghĩa VKOR của con người cho thấy sự phong phú hoặc hoạt động giảm, có thể gây ra tình trạng nhạy cảm với warfarin hoặc gây bệnh."} +{"idx": 1870472, "en": "BACKGROUND Psychiatry is nearly entirely reliant on patient self-reporting, and there are few objective and reliable tests or sources of collateral information available to help diagnostic and assessment procedures. Technology offers opportunities to collect objective digital data to complement patient experience and facilitate more informed treatment decisions. OBJECTIVE We aimed to develop computational algorithms based on internet search activity designed to support diagnostic procedures and relapse identification in individuals with schizophrenia spectrum disorders. METHODS We extracted 32,733 time-stamped search queries across 42 participants with schizophrenia spectrum disorders and 74 healthy volunteers between the ages of 15 and 35 (mean 24.4 years, 44.0% male), and built machine-learning diagnostic and relapse classifiers utilizing the timing, frequency, and content of online search activity. RESULTS Classifiers predicted a diagnosis of schizophrenia spectrum disorders with an area under the curve value of 0.74 and predicted a psychotic relapse in individuals with schizophrenia spectrum disorders with an area under the curve of 0.71. Compared with healthy participants, those with schizophrenia spectrum disorders made fewer searches and their searches consisted of fewer words. Prior to a relapse hospitalization, participants with schizophrenia spectrum disorders were more likely to use words related to hearing, perception, and anger, and were less likely to use words related to health. CONCLUSIONS Online search activity holds promise for gathering objective and easily accessed indicators of psychiatric symptoms. Utilizing search activity as collateral behavioral health information would represent a major advancement in efforts to capitalize on objective digital data to improve mental health monitoring.", "gemini": "BỐI CẢNH Tâm thần học gần như hoàn toàn dựa vào việc tự báo cáo của bệnh nhân và có rất ít xét nghiệm khách quan và đáng tin cậy hoặc các nguồn thông tin phụ trợ có sẵn để hỗ trợ các quy trình chẩn đoán và đánh giá. Công nghệ cung cấp các cơ hội để thu thập dữ liệu kỹ thuật số khách quan để bổ sung cho kinh nghiệm của bệnh nhân và tạo điều kiện cho các quyết định điều trị được thông báo nhiều hơn. MỤC TIÊU Chúng tôi nhằm mục đích phát triển các thuật toán tính toán dựa trên hoạt động tìm kiếm trên internet được thiết kế để hỗ trợ các quy trình chẩn đoán và xác định tái phát ở những người mắc các rối loạn phổ tâm thần phân liệt. PHƯƠNG PHÁP Chúng tôi đã trích xuất 32.733 truy vấn tìm kiếm có dấu thời gian trên 42 người tham gia mắc các rối loạn phổ tâm thần phân liệt và 74 tình nguyện viên khỏe mạnh trong độ tuổi từ 15 đến 35 (trung bình 24,4 tuổi, 44,0% nam) và xây dựng bộ phân loại chẩn đoán và tái phát học máy sử dụng thời gian, tần suất và nội dung của hoạt động tìm kiếm trực tuyến. KẾT QUẢ Bộ phân loại dự đoán chẩn đoán các rối loạn phổ tâm thần phân liệt với giá trị diện tích dưới đường cong là 0,74 và dự đoán tái phát loạn thần ở những người mắc các rối loạn phổ tâm thần phân liệt với diện tích dưới đường cong là 0,71. So với những người tham gia khỏe mạnh, những người mắc các rối loạn phổ tâm thần phân liệt thực hiện ít tìm kiếm hơn và các tìm kiếm của họ bao gồm ít từ hơn. Trước khi tái nhập viện, những người tham gia mắc các rối loạn phổ tâm thần phân liệt có nhiều khả năng sử dụng các từ liên quan đến thính giác, nhận thức và tức giận, và ít có khả năng sử dụng các từ liên quan đến sức khỏe. KẾT LUẬN Hoạt động tìm kiếm trực tuyến hứa hẹn sẽ thu thập các chỉ số khách quan và dễ truy cập về các triệu chứng tâm thần. Việc sử dụng hoạt động tìm kiếm làm thông tin sức khỏe hành vi phụ trợ sẽ đại diện cho một bước tiến lớn trong các nỗ lực tận dụng dữ liệu kỹ thuật số khách quan để cải thiện việc theo dõi sức khỏe tâm thần."} +{"idx": 1870473, "en": "BACKGROUND Down Your Drink (DYD) is a widely used unguided web-based alcohol moderation program for the general public based on cognitive behavioral therapy (CBT) and motivational interviewing (MI); it provides users with many opportunities to enter free-text responses. OBJECTIVE The aim of this study was to assess participants' use of key CBT and MI components, the presence of change talk and sustain talk within their responses, and whether these data are associated with drinking outcomes after 3 months. METHODS An exploratory secondary data analysis was conducted on data collected in 2008 from the definitive randomized trial of DYD (N=503). Past week alcohol use at baseline and 3-month follow-up were measured with the TOT-AL. Covariates included baseline alcohol use, age, gender, education level, and word count of the responses. Use of MI and CBT components and presence of change talk and sustain talk were coded by two independent coders (Cohen κ range 0.91-1). Linear model regressions on the subsample of active users (n=410) are presented along with a negative binomial regression. RESULTS The most commonly used component was the listing of pros and cons of drinking. The number of listed high-risk situations was associated with lower alcohol use at 3-month follow-up (Badj -2.15, 95% CI -3.92 to -0.38, P=.02). Findings on the effects of the percentage of change talk and the number of listed strategies to deal with high-risk situations were inconsistent. CONCLUSIONS An unguided web-based alcohol moderation program can elicit change talk and sustain talk. This secondary analysis suggests that the number of listed high-risk situations can predict alcohol use at 3-month follow-up. Other components show inconsistent findings and should be studied further.", "gemini": "BỐI CẢNH Down Your Drink (DYD) là một chương trình điều độ rượu trực tuyến không hướng dẫn được sử dụng rộng rãi dành cho công chúng dựa trên liệu pháp hành vi nhận thức (CBT) và phỏng vấn động lực (MI); nó cung cấp cho người dùng nhiều cơ hội để nhập các phản hồi văn bản tự do. MỤC TIÊU Mục đích của nghiên cứu này là đánh giá việc người tham gia sử dụng các thành phần chính của CBT và MI, sự hiện diện của cuộc trò chuyện thay đổi và cuộc trò chuyện duy trì trong các phản hồi của họ và liệu dữ liệu này có liên quan đến kết quả uống rượu sau 3 tháng hay không. PHƯƠNG PHÁP Một phân tích dữ liệu thứ cấp thăm dò đã được thực hiện trên dữ liệu thu thập được vào năm 2008 từ thử nghiệm ngẫu nhiên xác định của DYD (N = 503). Việc sử dụng rượu trong tuần trước khi bắt đầu và theo dõi trong 3 tháng được đo bằng TOT-AL. Các biến phụ bao gồm mức sử dụng rượu ban đầu, tuổi, giới tính, trình độ học vấn và số lượng từ trong các phản hồi. Việc sử dụng các thành phần MI và CBT và sự hiện diện của cuộc trò chuyện thay đổi và cuộc trò chuyện duy trì được mã hóa bởi hai người mã hóa độc lập (phạm vi Cohen κ 0,91-1). Các hồi quy mô hình tuyến tính trên phân nhóm người dùng tích cực (n = 410) được trình bày cùng với hồi quy nhị thức âm. KẾT QUẢ Thành phần được sử dụng phổ biến nhất là liệt kê các ưu và nhược điểm của việc uống rượu. Số lượng các tình huống nguy cơ cao được liệt kê có liên quan đến việc sử dụng rượu thấp hơn khi theo dõi trong 3 tháng (Badj -2,15, KTCB 95% -3,92 đến -0,38, P = 0,02). Các phát hiện về tác động của tỷ lệ phần trăm cuộc trò chuyện thay đổi và số lượng các chiến lược được liệt kê để đối phó với các tình huống nguy cơ cao không nhất quán. KẾT LUẬN Một chương trình điều độ rượu trực tuyến không hướng dẫn có thể gợi ra cuộc trò chuyện thay đổi và cuộc trò chuyện duy trì. Phân tích thứ cấp này cho thấy số lượng các tình huống nguy cơ cao được liệt kê có thể dự đoán mức sử dụng rượu khi theo dõi trong 3 tháng. Các thành phần khác cho thấy những phát hiện không nhất quán và cần được nghiên cứu thêm."} +{"idx": 1870474, "en": "OBJECTIVE Metabolic syndrome (MetS) includes several cardiovascular (CV) risk factors. This study aimed to assess CV risk of MetS, contribution of its components to the risk, and whether MetS provides additional risk beyond its components. METHODS The Prospective Urban Rural Epidemiology (PURE) Turkey cohort included 3933 individuals aged between 35 and 70 years, with a median follow-up of 8.9 years. MetS was diagnosed as the presence of any of the following criteria: high blood pressure, high fasting plasma glucose, abdominal obesity, low HDL-cholesterol, or high triglycerides. The primary outcome was the composite of fatal CV events, non-fatal myocardial infarction, stroke or heart failure, adjusted for age, sex, smoking, family history of CV diseases, and LDL-cholesterol. RESULTS The primary outcome was more common in the MetS group [178 (9.2%) vs. 70 (3.5%); corresponding incidence rate of 11.3 vs. 4.2 per 1000 person-years; log-rank p<0.001]. Each component was significantly associated with the primary outcome; however, when the components were sequentially included in the model, abdominal obesity and high triglycerides did not provide additional risk on top of the other three components. The hazard ratio for MetS for the primary outcome was 2.12 (95% confidence interval 1.59-2.81, p<0.001), and the discriminative ability (c-statistics) of the models with MetS and the components was similar. CONCLUSION MetS increases the risk of CV events more than two-fold. High blood pressure, high fasting plasma glucose, and low HDL-cholesterol are the top three components of MetS for CV risk. MetS and its components have a similar discriminative ability for CV events.", "gemini": "MỤC TIÊU Hội chứng chuyển hóa (MetS) bao gồm một số yếu tố nguy cơ tim mạch (CV). Nghiên cứu này nhằm đánh giá nguy cơ CV của MetS, sự đóng góp của các thành phần của nó vào nguy cơ và liệu MetS có cung cấp thêm nguy cơ ngoài các thành phần của nó hay không. PHƯƠNG PHÁP Đội nghiên cứu dịch tễ học đô thị nông thôn triển vọng (PURE) Thổ Nhĩ Kỳ bao gồm 3933 cá nhân trong độ tuổi từ 35 đến 70, với thời gian theo dõi trung bình là 8,9 năm. MetS được chẩn đoán là sự hiện diện của bất kỳ tiêu chuẩn nào sau đây: huyết áp cao, lượng đường huyết lúc đói cao, béo phì bụng, cholesterol HDL thấp hoặc lượng triglyceride cao. Kết quả chính là sự kết hợp của các biến cố CV gây tử vong, nhồi máu cơ tim không gây tử vong, đột quỵ hoặc suy tim, được điều chỉnh theo tuổi, giới tính, hút thuốc, tiền sử gia đình mắc bệnh CV và cholesterol LDL. KẾT QUẢ Kết quả chính phổ biến hơn ở nhóm MetS [178 (9,2%) so với 70 (3,5%); tỷ lệ mắc tương ứng là 11,3 so với 4,2 trên 1000 người-năm; log-rank p < 0,001]. Mỗi thành phần có liên quan đáng kể đến kết quả chính; tuy nhiên, khi các thành phần được đưa vào mô hình theo trình tự, béo phì bụng và lượng triglyceride cao không cung cấp thêm nguy cơ ngoài ba thành phần khác. Tỷ lệ nguy cơ đối với MetS đối với kết quả chính là 2,12 (khoảng tin cậy 95% 1,59-2,81, p < 0,001) và khả năng phân biệt (thống kê c) của các mô hình có MetS và các thành phần là tương tự nhau. KẾT LUẬN MetS làm tăng nguy cơ các biến cố CV hơn gấp đôi. Huyết áp cao, lượng đường huyết lúc đói cao và cholesterol HDL thấp là ba thành phần hàng đầu của MetS đối với nguy cơ CV. MetS và các thành phần của nó có khả năng phân biệt tương tự đối với các biến cố CV."} +{"idx": 1870475, "en": "OBJECTIVE Many countries are facing a shortage of cardiac surgeons, who are crucial in meeting the demands of growing number of patients in need of coronary artery bypass grafting. This situation poses a serious challenge, especially in China. The purpose of this study is to determine whether cardiac surgeons are suitable for training in coronary artery anastomosis at an earlier stage in their career. METHODS We divided 12 cardiac surgeons with no prior experience in coronary artery anastomosis into senior and junior groups for training and assessment. All trainees received training in coronary artery anastomosis for a defined period. We performed in vivo and in vitro examinations before and after training, respectively. Additionally, we assessed individual surgical performance of surgeons by using performance rating scores, including different aspects of surgical skills rated on a five-point scale. RESULTS The post-training scores (overall, junior, senior) were significantly higher than the pre-training scores (overall, junior, and senior). We observed no differences in pre-training and post-training scores between the junior and senior groups. CONCLUSION Senior surgeons did not had any significant advantages over junior surgeons with respect to coronary artery anastomosis in the absence of training. Junior surgeons achieved the same results as the senior surgeons after training.", "gemini": "MỤC TIÊU Nhiều quốc gia đang phải đối mặt với tình trạng thiếu hụt bác sĩ phẫu thuật tim, những người đóng vai trò rất quan trọng trong việc đáp ứng nhu cầu của số lượng bệnh nhân ngày càng tăng cần ghép bắc cầu động mạch vành. Tình trạng này đặt ra một thách thức nghiêm trọng, đặc biệt là ở Trung Quốc. Mục đích của nghiên cứu này là xác định xem các bác sĩ phẫu thuật tim có phù hợp để đào tạo về nối động mạch vành ở giai đoạn sớm hơn trong sự nghiệp của họ hay không. PHƯƠNG PHÁP Chúng tôi chia 12 bác sĩ phẫu thuật tim chưa có kinh nghiệm về nối động mạch vành thành nhóm cao cấp và nhóm cơ sở để đào tạo và đánh giá. Tất cả những người được đào tạo đều được đào tạo về nối động mạch vành trong một thời gian nhất định. Chúng tôi đã tiến hành các cuộc kiểm tra trong cơ thể sống và trong ống nghiệm trước và sau khi đào tạo. Ngoài ra, chúng tôi đánh giá hiệu suất phẫu thuật riêng lẻ của các bác sĩ phẫu thuật bằng cách sử dụng điểm đánh giá hiệu suất, bao gồm các khía cạnh khác nhau của kỹ năng phẫu thuật được đánh giá trên thang điểm năm. KẾT QUẢ Điểm sau đào tạo (tổng thể, cơ sở, cao cấp) cao hơn đáng kể so với điểm trước đào tạo (tổng thể, cơ sở và cao cấp). Chúng tôi không quan sát thấy sự khác biệt về điểm trước và sau đào tạo giữa nhóm cơ sở và nhóm cao cấp. KẾT LUẬN Các bác sĩ phẫu thuật cao cấp không có bất kỳ lợi thế đáng kể nào so với các bác sĩ phẫu thuật cơ sở về mặt nối động mạch vành khi không được đào tạo. Các bác sĩ phẫu thuật cơ sở đạt được kết quả tương tự như các bác sĩ phẫu thuật cao cấp sau khi được đào tạo."} +{"idx": 1870476, "en": "OBJECTIVE Acute coronary syndrome (ACS) is a leading cause of death worldwide. There is great interest in defining the risk factors and underlying mechanisms of ACS among young people. The microbiota and its metabolites have recently become a popular research topic, yet there is still no study that investigated microbiota-generated metabolites as a possible risk factor in young patients with ACS. In this study, we aimed to investigate the relationship between microbiota-generated metabolites and ACS in young people. METHODS This study included 44 young patients with ACS (<50 years of age), 39 elderly patients with ACS, and 44 patients with normal coronary arteries. Inflammatory parameters and serum trimethylamine N-oxide (TMAO) and choline levels were measured in all patients. RESULTS Young patients with ACS had significantly higher levels of TMAO and choline compared to the control and elderly ACS groups. Also, elderly patients with ACS had a significantly higher level of TMAO than the control group. Linear regression analysis was performed to determine the independent predictors of TMAO. Two regression models were involved. The first model included young ACS and control groups, while the second model included young and elderly ACS groups. In the first model, we found that young ACS (ß=0.399, p=0.004) and smoking ACS (ß=0.211, p=0.046) were significantly associated with TMAO level. In the second model, young ACS was significantly associated with TMAO level (ß=0.230, p=0.035). CONCLUSION In this study, we have shown that young ACS was significantly associated with increased TMAO level.", "gemini": "MỤC TIÊU Hội chứng vành cấp (ACS) là nguyên nhân gây tử vong hàng đầu trên toàn thế giới. Có rất nhiều mối quan tâm trong việc xác định các yếu tố nguy cơ và cơ chế tiềm ẩn của ACS ở những người trẻ tuổi. Hệ vi sinh vật và các chất chuyển hóa của nó gần đây đã trở thành một chủ đề nghiên cứu phổ biến, nhưng vẫn chưa có nghiên cứu nào điều tra các chất chuyển hóa do hệ vi sinh vật tạo ra như một yếu tố nguy cơ có thể xảy ra ở những bệnh nhân trẻ mắc ACS. Trong nghiên cứu này, chúng tôi nhằm mục đích điều tra mối quan hệ giữa các chất chuyển hóa do hệ vi sinh vật tạo ra và ACS ở những người trẻ tuổi. PHƯƠNG PHÁP Nghiên cứu này bao gồm 44 bệnh nhân trẻ mắc ACS (<50 tuổi), 39 bệnh nhân cao tuổi mắc ACS và 44 bệnh nhân có động mạch vành bình thường. Các thông số viêm và trimethylamine N-oxide (TMAO) trong huyết thanh và nồng độ choline được đo ở tất cả bệnh nhân. KẾT QUẢ Những bệnh nhân trẻ mắc ACS có nồng độ TMAO và choline cao hơn đáng kể so với nhóm đối chứng và nhóm ACS cao tuổi. Ngoài ra, bệnh nhân cao tuổi mắc ACS có nồng độ TMAO cao hơn đáng kể so với nhóm đối chứng. Phân tích hồi quy tuyến tính được thực hiện để xác định các yếu tố dự báo độc lập của TMAO. Hai mô hình hồi quy đã được sử dụng. Mô hình đầu tiên bao gồm nhóm ACS trẻ và nhóm đối chứng, trong khi mô hình thứ hai bao gồm nhóm ACS trẻ và cao tuổi. Trong mô hình đầu tiên, chúng tôi thấy rằng ACS trẻ (ß = 0,399, p = 0,004) và ACS hút thuốc (ß = 0,211, p = 0,046) có liên quan đáng kể đến mức TMAO. Trong mô hình thứ hai, ACS trẻ có liên quan đáng kể đến mức TMAO (ß = 0,230, p = 0,035). KẾT LUẬN Trong nghiên cứu này, chúng tôi đã chỉ ra rằng ACS trẻ có liên quan đáng kể đến việc tăng mức TMAO."} +{"idx": 1870477, "en": "OBJECTIVE To study the prevalence of cardiovascular (CV) risk factors (RFs) in the rural population of the Chui region of Kyrgyzstan (Central Asia). METHODS The sample was representative of the population in terms of age and sex and included at least 10% of the population aged 18-65 y. Of the 1,672 people included in the cohort, 1.330 people responded to the invitation (79.5% of the total sample population). All study participants were interviewed using standardized questionnaires and examined by a cardiologist. Blood pressure (BP), weight, height, waist circumference (WC), fasting serum glucose, and fasting lipid level were measured. RESULTS The prevalence of major CV RFs in the examined sample was as follows: arterial hypertension 34.1%, obesity 25.7%, and abdominal obesity 52.3%; the factors were significantly more prevalent in women (68.2%) and increased with age. The frequency of lipid metabolism disorders was 88.4% in the examined subjects, and an increased level of low-density cholesterol (70.5%) was common. Hypodynamia was detected in 15.6% of the subjects, diabetes mellitus in 3.76%, and a family history of cardiovascular disease was present in 34.8% of the examined subjects. The frequency of smoking was 24.6% and was significantly higher in men (46.9%). CONCLUSION Abdominal obesity, followed by hypercholesterolemia and arterial hypertension were the most common RFs among the rural population of the Chui region of Kyrgyzstan. Smoking was the most common RF among men. The prevalence of traditional CV RFs, except smoking, increased with age.", "gemini": "MỤC TIÊU Nghiên cứu tỷ lệ mắc các yếu tố nguy cơ tim mạch (CV) trong cộng đồng dân cư nông thôn của vùng Chui thuộc Kyrgyzstan (Trung Á). PHƯƠNG PHÁP Mẫu nghiên cứu đại diện cho dân số về mặt tuổi tác và giới tính và bao gồm ít nhất 10% dân số trong độ tuổi từ 18-65. Trong số 1.672 người tham gia nhóm nghiên cứu, 1.330 người đã trả lời lời mời (chiếm 79,5% tổng số mẫu nghiên cứu). Tất cả những người tham gia nghiên cứu đều được phỏng vấn bằng các bảng câu hỏi chuẩn hóa và được bác sĩ tim mạch khám. Huyết áp (BP), cân nặng, chiều cao, chu vi vòng eo (WC), đường huyết lúc đói và mức lipid lúc đói đã được đo. KẾT QUẢ Tỷ lệ mắc các yếu tố nguy cơ CV chính trong mẫu nghiên cứu được như sau: tăng huyết áp động mạch 34,1%, béo phì 25,7% và béo bụng 52,3%; các yếu tố này phổ biến hơn đáng kể ở phụ nữ (68,2%) và tăng theo tuổi. Tần suất rối loạn chuyển hóa lipid là 88,4% ở những đối tượng được nghiên cứu và mức cholesterol tỷ trọng thấp tăng (70,5%) là phổ biến. Hạ động lực được phát hiện ở 15,6% đối tượng, đái tháo đường ở 3,76% và tiền sử gia đình mắc bệnh tim mạch ở 34,8% đối tượng được nghiên cứu. Tần suất hút thuốc là 24,6% và cao hơn đáng kể ở nam giới (46,9%). KẾT LUẬN Béo bụng, tiếp theo là tăng cholesterol máu và tăng huyết áp động mạch là những yếu tố nguy cơ phổ biến nhất trong cộng đồng dân cư nông thôn của vùng Chui thuộc Kyrgyzstan. Hút thuốc là yếu tố nguy cơ phổ biến nhất ở nam giới. Tỷ lệ mắc các yếu tố nguy cơ CV truyền thống, ngoại trừ hút thuốc, tăng theo tuổi."} +{"idx": 1870478, "en": "Myocardial ischemia resulting from psychological stress [mental stress-induced myocardial ischemia (MSIMI)] refers to the condition wherein psychosocial and psychological stimulations cause myocardial ischemia in patients with coronary heart disease, which is different from drug-induced myocardial ischemia. Therefore, this condition often escapes diagnosis, portends clinical risk, and affects the quality of life of MSIMI survivors. MSIMI is closely related to the poor prognosis of cardiovascular diseases, especially in young women, according to recent randomized, controlled trials (RCTs) on MSIMI. These RCTs involved different sample sizes, interventional measures, and detection techniques. Moreover, differences exist regarding the prevalence rate, distribution characteristics, possible pathogenesis, and clinical significance. Nevertheless, currently, the diagnostic criteria, pathogenesis, and treatment of MSIMI are still in the clinical exploration stage. Hence, considering recent RCTs, this paper summarizes the research status of MSIMI from the aspects of pathogenesis, diagnosis, and treatment strategies to provide a theoretical basis for the follow-up diagnostic methods and treatment guidelines for MSIMI.", "gemini": "Thiếu máu cơ tim do căng thẳng tâm lý [thiếu máu cơ tim do căng thẳng về tinh thần (MSIMI)] đề cập đến tình trạng mà các kích thích tâm lý xã hội và tâm lý gây ra tình trạng thiếu máu cơ tim ở những bệnh nhân mắc bệnh tim mạch vành, khác với tình trạng thiếu máu cơ tim do thuốc gây ra. Do đó, tình trạng này thường thoát khỏi chẩn đoán, biểu hiện rủi ro lâm sàng và ảnh hưởng đến chất lượng cuộc sống của những người sống sót sau MSIMI. MSIMI có liên quan chặt chẽ đến tiên lượng kém của các bệnh tim mạch, đặc biệt là ở phụ nữ trẻ, theo các thử nghiệm có đối chứng ngẫu nhiên (RCT) gần đây về MSIMI. Các RCT này liên quan đến các kích thước mẫu khác nhau, các biện pháp can thiệp và các kỹ thuật phát hiện. Hơn nữa, có sự khác biệt về tỷ lệ mắc bệnh, đặc điểm phân bố, khả năng sinh bệnh và ý nghĩa lâm sàng. Tuy nhiên, hiện nay, các tiêu chuẩn chẩn đoán, sinh bệnh học và điều trị MSIMI vẫn đang trong giai đoạn thăm dò lâm sàng. Do đó, xem xét các RCT gần đây, bài báo này tóm tắt tình trạng nghiên cứu của MSIMI từ các khía cạnh sinh bệnh học, chẩn đoán và các chiến lược điều trị để cung cấp cơ sở lý thuyết cho các phương pháp chẩn đoán theo dõi và hướng dẫn điều trị MSIMI."} +{"idx": 1870479, "en": "Due to the inner filter effect (IFE) between Brilliant Blue (BB) and gold nanoclusters (AuNCs), a simple and rapid approach for BB detection was developed. Since the absorption spectrum of BB has a good overlap with the emission spectrum of the AuNCs, the fluorescence of the AuNC solution was quenched after adding BB. Under optimal conditions, a good linear relationship for detection of BB was obtained from 0.05 to 7.5 μM, and the detection limit was 0.0167 μM. More importantly, the assay was successfully applied to assess BB in food samples such as hard candy, chocolate and dried blueberries, showing its potential in food quality control in the future.", "gemini": "Do hiệu ứng bộ lọc bên trong (IFE) giữa Brilliant Blue (BB) và các cụm nano vàng (AuNCs), một phương pháp tiếp cận đơn giản và nhanh chóng để phát hiện BB đã được phát triển. Do phổ hấp thụ của BB có sự chồng chéo tốt với phổ phát xạ của AuNCs nên huỳnh quang của dung dịch AuNCs bị dập tắt sau khi thêm BB. Trong điều kiện tối ưu, thu được mối quan hệ tuyến tính tốt để phát hiện BB từ 0,05 đến 7,5 μM và giới hạn phát hiện là 0,0167 μM. Quan trọng hơn, xét nghiệm đã được áp dụng thành công để đánh giá BB trong các mẫu thực phẩm như kẹo cứng, sô cô la và quả việt quất khô, cho thấy tiềm năng của nó trong việc kiểm soát chất lượng thực phẩm trong tương lai."} +{"idx": 1870480, "en": "We presented a novel red-emission fluorescent probe (MSO) for selectively monitoring lysosomal pH fluctuation in living cells. The probe was designed by employing rhodamine B as the off-on pH sensitive moiety owing to the unique spirocycle group and morpholine as the lysosome targetable unit. Based on the H+-induced spirocyclic ring opening process, MSO displayed significant pH sensing properties around 590 nm, with a pKa value of 5.42 and a good linear pH response ranging from 5.00 to 6.00. Besides, the probe possessed other prominent photophysical properties such as good selectivity and excellent photostability as well as low cytotoxicity, together making the red-emission probe more favorable for long-time and real-time imaging in live cells. Furthermore, MSO selectively accumulated into lysosomes and successfully visualized the mitophagy, cell apoptosis and heat shock processes by monitoring the rise of lysosomal pH.", "gemini": "Chúng tôi đã trình bày một đầu dò huỳnh quang phát xạ đỏ mới (MSO) để theo dõi chọn lọc sự biến động pH lysosome trong các tế bào sống. Đầu dò được thiết kế bằng cách sử dụng rhodamine B làm phần tử nhạy pH tắt-bật do nhóm spirocycle độc đáo và morpholine làm đơn vị nhắm mục tiêu lysosome. Dựa trên quá trình mở vòng spirocyclic do H+ tạo ra, MSO hiển thị các đặc tính cảm biến pH đáng kể xung quanh 590 nm, với giá trị pKa là 5,42 và đáp ứng pH tuyến tính tốt trong khoảng từ 5,00 đến 6,00. Bên cạnh đó, đầu dò sở hữu các đặc tính quang lý nổi bật khác như độ chọn lọc tốt và độ ổn định quang tuyệt vời cũng như độc tính tế bào thấp, cùng nhau làm cho đầu dò phát xạ đỏ thuận lợi hơn cho việc chụp ảnh thời gian thực và thời gian dài trong các tế bào sống. Hơn nữa, MSO tích tụ chọn lọc vào lysosome và hình dung thành công quá trình mitophagy, quá trình apoptosis tế bào và quá trình sốc nhiệt bằng cách theo dõi sự gia tăng pH lysosome."} +{"idx": 1870481, "en": "As a class of ideal fluorescent nanomaterials, self-assembled copper nanoclusters (CuNCs) have attracted increasing interest. Unfortunately, most of these CuNCs only possessed bright luminescence in acidic solution, which limited their practical applications in a physiological environment. Retaining the strong fluorescence of these CuNCs in neutral or alkaline solution is still a challenging task. In this strategy, self-assembled CuNCs were prepared by using 4-methylthiophenol as the protecting ligand. The self-assembled CuNCs display stable and bright luminescence with excitation/emission maxima at 330/605 nm even in neutral and alkaline environments. Interestingly, with the addition of glutathione (GSH), the fluorescence intensity of CuNCs is enhanced strongly through the GSH-controlled aggregation-induced emission enhancement of self-assembled CuNCs. The turn-on fluorescence strategy can determine the GSH concentration in the range from 1 to 100 μM with a limit of detection of 300 nM. In addition, the method is employed for the determination of GSH levels in cells. Therefore, the turn-on fluorescence strategy is reliable, sensitive and suitable for the determination of cellular GSH levels.", "gemini": "Là một lớp vật liệu nano huỳnh quang lý tưởng, các cụm nano đồng tự lắp ráp (CuNC) đã thu hút được sự quan tâm ngày càng tăng. Thật không may, hầu hết các CuNC này chỉ phát ra ánh sáng mạnh trong dung dịch axit, điều này hạn chế các ứng dụng thực tế của chúng trong môi trường sinh lý. Duy trì khả năng phát huỳnh quang mạnh của các CuNC này trong dung dịch trung tính hoặc kiềm vẫn là một nhiệm vụ đầy thách thức. Trong chiến lược này, các CuNC tự lắp ráp được chế tạo bằng cách sử dụng 4-methylthiophenol làm phối tử bảo vệ. Các CuNC tự lắp ráp thể hiện khả năng phát quang ổn định và mạnh với cực đại kích thích/phát xạ ở 330/605 nm ngay cả trong môi trường trung tính và kiềm. Điều thú vị là với việc bổ sung glutathione (GSH), cường độ huỳnh quang của CuNC được tăng cường mạnh mẽ thông qua sự tăng cường phát xạ do kết tập do GSH kiểm soát của các CuNC tự lắp ráp. Chiến lược huỳnh quang bật có thể xác định nồng độ GSH trong khoảng từ 1 đến 100 μM với giới hạn phát hiện là 300 nM. Ngoài ra, phương pháp này được sử dụng để xác định nồng độ GSH trong tế bào. Do đó, chiến lược huỳnh quang bật là đáng tin cậy, nhạy và phù hợp để xác định nồng độ GSH của tế bào."} +{"idx": 1870482, "en": "A ZnII complex based on a luminescent organic radical was doped into host molecular crystals. The 5, 10, and 20 wt%-doped crystals showed excimer emissions and their luminescent behaviours were significantly modulated by an external magnetic field. These are the first examples showing excimer emissions and magnetic-field-sensitive luminescent properties for complexes based on luminescent radicals. The excimer species contributing to magnetoluminescence was determined by analyzing the emission spectra and their magnetic-field dependencies. These results suggest the general nature of magnetic field effects on the luminescence of radicals as well as the importance of the type of interaction between radicals.", "gemini": "Một phức hợp ZnII dựa trên gốc hữu cơ phát quang đã được đưa vào các tinh thể phân tử chủ. Các tinh thể được pha tạp 5, 10 và 20 wt% cho thấy phát xạ excimer và hành vi phát quang của chúng bị điều chế đáng kể bởi từ trường bên ngoài. Đây là những ví dụ đầu tiên cho thấy phát xạ excimer và các đặc tính phát quang nhạy từ trường đối với các phức hợp dựa trên các gốc phát quang. Các loài excimer góp phần vào phát quang từ được xác định bằng cách phân tích phổ phát xạ và sự phụ thuộc vào từ trường của chúng. Những kết quả này cho thấy bản chất chung của các hiệu ứng từ trường đối với sự phát quang của các gốc cũng như tầm quan trọng của loại tương tác giữa các gốc."} +{"idx": 1870483, "en": "Here we report that incorporation of an abasic site to DNA minidumbbells formed by natural sequences can lead to significant enhancements in their thermodynamic stability. Based on these stable minidumbbells, the first metal ion-controlled molecular switch which can regulate instant and reversible DNA duplex formation and dissociation has been constructed.", "gemini": "Ở đây chúng tôi báo cáo rằng việc kết hợp một vị trí abasic với các phân tử DNA minidumbbell được hình thành bởi các trình tự tự nhiên có thể dẫn đến sự tăng cường đáng kể về độ ổn định nhiệt động của chúng. Dựa trên các phân tử minidumbbell ổn định này, công tắc phân tử điều khiển ion kim loại đầu tiên có thể điều chỉnh sự hình thành và phân ly DNA duplex tức thời và có thể đảo ngược đã được xây dựng."} +{"idx": 1870484, "en": "Histidine can trigger bimetallic gold/copper nanoclusters to turn on strong red fluoresence, which is capable of sensing nanomolar levels of histidine selectively.", "gemini": "Histidine có thể kích hoạt các nano cụm vàng/đồng bimetallic để tạo ra huỳnh quang đỏ mạnh, có khả năng cảm biến các mức nanomolar của histidine một cách chọn lọc."} +{"idx": 1870485, "en": "Herein we describe a method for inducing cancer cell death, which relies on the use of a H2O2-responsive glycan metabolic precursor in conjunction with antibody-dependent cellular cytotoxicity (ADCC) or photodynamic therapy (PDT).", "gemini": "Trong bài báo này, chúng tôi mô tả một phương pháp gây chết tế bào ung thư, dựa trên việc sử dụng tiền chất chuyển hóa glycan phản ứng với H2O2 kết hợp với tế bào phụ thuộc kháng thể (ADCC) hoặc liệu pháp quang động (PDT)."} +{"idx": 1870486, "en": "In the search for new sources of antimicrobials many researchers have investigated antimicrobial peptides (AMPs) as templates for the design of innovative therapeutics. However, efforts to develop AMPs in this area has been severely hampered by their inherent susceptibility to enzymatic degradation. Given this only a handful of AMPs are currently in clinical trials. Peptide mimetics such as peptoids have emerged as highly promising alternatives to AMPs as they are inherently stable to enzymatic degradation and display potent antimicrobial properties. This feature article highlights the progress that has been made towards the development of novel anti-infective peptoids.", "gemini": "Trong quá trình tìm kiếm các nguồn kháng sinh mới, nhiều nhà nghiên cứu đã nghiên cứu các peptide kháng khuẩn (AMP) làm khuôn mẫu để thiết kế các phương pháp điều trị sáng tạo. Tuy nhiên, những nỗ lực phát triển AMPs trong lĩnh vực này đã bị cản trở nghiêm trọng do tính nhạy cảm vốn có của chúng đối với sự phân hủy do enzym. Do đó, chỉ có một số ít AMPs hiện đang trong các thử nghiệm lâm sàng. Các chất bắt chước peptide như peptoid đã nổi lên như những chất thay thế đầy hứa hẹn cho AMPs vì chúng vốn ổn định với sự phân hủy do enzym và thể hiện các đặc tính kháng khuẩn mạnh. Bài báo chuyên đề này nêu bật tiến trình đã đạt được hướng tới việc phát triển các peptoid chống nhiễm trùng mới."} +{"idx": 1870487, "en": "In this perspective article we highlight research opportunities and challenges in probing structural dynamics of molecular systems using X-ray Photon Correlation Spectroscopy (XPCS). The development of new X-ray sources, such as 4th generation storage rings and X-ray free-electron lasers (XFELs), provides promising new insights into molecular motion. Employing XPCS at these sources allows to capture a very broad range of timescales and lengthscales, spanning from femtoseconds to minutes and atomic scales to the mesoscale. Here, we discuss the scientific questions that can be addressed with these novel tools for two prominent examples: the dynamics of proteins in biomolecular condensates and the dynamics of supercooled water. Finally, we provide practical tips for designing and estimating feasibility of XPCS experiments as well as on detecting and mitigating radiation damage.", "gemini": "Trong bài báo quan điểm này, chúng tôi nêu bật các cơ hội và thách thức nghiên cứu trong việc thăm dò động lực học cấu trúc của các hệ thống phân tử bằng cách sử dụng Phổ tương quan photon tia X (XPCS). Sự phát triển của các nguồn tia X mới, chẳng hạn như vòng lưu trữ thế hệ thứ 4 và tia laser điện tử tự do tia X (XFEL), cung cấp những hiểu biết mới đầy hứa hẹn về chuyển động phân tử. Việc sử dụng XPCS tại các nguồn này cho phép nắm bắt một phạm vi rất rộng về thang thời gian và thang độ dài, kéo dài từ femto giây đến phút và thang nguyên tử đến thang trung bình. Tại đây, chúng tôi thảo luận về các câu hỏi khoa học có thể được giải quyết bằng các công cụ mới này cho hai ví dụ nổi bật: động lực học của protein trong ngưng tụ sinh học phân tử và động lực học của nước quá lạnh. Cuối cùng, chúng tôi cung cấp các mẹo thực tế để thiết kế và ước tính khả thi của các thí nghiệm XPCS cũng như về việc phát hiện và giảm thiểu thiệt hại do bức xạ."} +{"idx": 1870488, "en": "The photoinduced cycloreversion of oxetanes has been thoroughly investigated in connection with the photorepair of the well-known DNA (6-4) photoproducts. In the present work, the direct photolysis of the two regioisomers arising from the irradiation of benzophenone (BP) and 1,3-dimethylthymine (DMT), namely the head-to-head (HH-1) and head-to-tail (HT-1) oxetane adducts, has been investigated by combining ultrafast spectroscopy and theoretical multiconfigurational quantum chemistry analysis. Both the experimental and computational results agree with the involvement of an excited triplet exciplex 3[BPDMT]* for the photoinduced oxetane cleavage to generate 3BP* and DMT through an adiabatic photochemical reaction. The experimental signature of 3[BPDMT]* is the appearance of an absorption band at ca. 400 nm, detected by femtosecond transient absorption spectroscopy. Its formation is markedly regioselective, as it is more efficient and proceeds faster for HH-1 (∼2.8 ps) than for HT-1 (∼6.3 ps). This is in line with the theoretical analysis, which predicts an energy barrier to reach the triplet exciplex for HT-1, in contrast with a less hindered profile for HH-1. Finally, the more favorable adiabatic cycloreversion of HH-1 compared to that of HT-1 is explained by its lower probability to reach the intersystem crossing with the ground state, which would induce a radiationless deactivation process leading either to a starting adduct or to a dissociated BP and DMT.", "gemini": "Sự đảo ngược vòng cycloreversion của oxetan đã được nghiên cứu kỹ lưỡng liên quan đến việc sửa chữa quang của các sản phẩm quang DNA (6-4) nổi tiếng. Trong công trình hiện tại, quá trình quang phân hủy trực tiếp của hai đồng phân vùng phát sinh từ sự chiếu xạ của benzophenone (BP) và 1,3-dimethylthymine (DMT), cụ thể là các adduct oxetan đầu-đầu (HH-1) và đầu-đuôi (HT-1), đã được nghiên cứu bằng cách kết hợp phổ học cực nhanh và phân tích hóa học lượng tử đa cấu hình lý thuyết. Cả kết quả thực nghiệm và tính toán đều nhất quán với sự tham gia của một exciplex ba trạng thái kích thích 3[BPDMT]* cho sự phân cắt oxetan do quang tạo ra 3BP* và DMT thông qua phản ứng quang hóa đoạn nhiệt. Dấu hiệu thực nghiệm của 3[BPDMT]* là sự xuất hiện của một dải hấp thụ ở khoảng 400 nm, được phát hiện bằng phổ hấp thụ thoáng qua femto giây. Sự hình thành của nó là rất rõ ràng, vì nó hiệu quả hơn và diễn ra nhanh hơn đối với HH-1 (∼2,8 ps) so với HT-1 (∼6,3 ps). Điều này phù hợp với phân tích lý thuyết, dự đoán một rào cản năng lượng để đạt được exciplex ba trạng thái đối với HT-1, trái ngược với cấu hình ít cản trở hơn đối với HH-1. Cuối cùng, sự đảo ngược vòng cycloreversion đoạn nhiệt thuận lợi hơn của HH-1 so với HT-1 được giải thích bằng xác suất thấp hơn của nó để đạt được sự giao cắt liên hệ với trạng thái cơ bản, điều này sẽ gây ra quá trình hủy hoạt tính không bức xạ dẫn đến adduct ban đầu hoặc BP và DMT bị tách rời."} +{"idx": 1870489, "en": "Elastomer, poly(n-butyl methacrylate), coated liquid metal (LM) nanodroplets (EGaIn@PBMA) were successfully fabricated via a facile in situ free radical polymerization method. The as-prepared soft nanoparticles can be directly hot-pressed into nanocomposites which not only exhibited ultra-high stretching flexibility (>400%) but also excellent dielectric properties with remarkably suppressed dielectric loss, showing great promise in the flexible energy-storage field.", "gemini": "Elastomer, poly(n-butyl methacrylate), các giọt nano kim loại lỏng (LM) phủ (EGaIn@PBMA) đã được chế tạo thành công thông qua phương pháp trùng hợp gốc tự do tại chỗ dễ dàng. Các hạt nano mềm như đã chế tạo có thể được ép nóng trực tiếp thành vật liệu nano tổng hợp không chỉ thể hiện tính linh hoạt kéo giãn cực cao (> 400%) mà còn có các tính chất điện môi tuyệt vời với tổn hao điện môi bị ức chế đáng kể, cho thấy triển vọng lớn trong lĩnh vực lưu trữ năng lượng linh hoạt."} +{"idx": 1870490, "en": "Light-enabled, AlCl3-catalyzed regioselective intramolecular nucleophilic cyclization of alkynes using non-nucleophilic alkyls as the nucleophile is reported. Upon photoexcitation, o-alkylphenyl alkynyl ketones can be transferred into (E)-photoenols. Thus, a nucleophilic methylene is formed from the non-nucleophilic alkyl. An AlCl3 catalyst can stabilize the (E)-photoenol intermediate and facilitate further intramolecular nucleophilic cyclization. DFT calculations indicated that the AlCl3-catalyzed cyclization is the regioselectivity determining step.", "gemini": "Chu trình hóa nội phân tử ái nhân của ankin được xúc tác bằng AlCl3, được kích hoạt bằng ánh sáng, sử dụng ankil không ái nhân làm ái nhân đã được báo cáo. Khi kích thích bằng ánh sáng, o-anlylphenyl ankinyl xeton có thể được chuyển thành (E)-photoenol. Do đó, một methylene ái nhân được hình thành từ ankil không ái nhân. Chất xúc tác AlCl3 có thể ổn định chất trung gian (E)-photoenol và tạo điều kiện thuận lợi cho chu trình hóa ái nhân nội phân tử tiếp theo. Các tính toán DFT chỉ ra rằng chu trình hóa xúc tác bằng AlCl3 là bước xác định tính chọn lọc vùng."} +{"idx": 1870491, "en": "Benefitting from their unique structure and physicochemical properties, two-dimensional (2D) materials have aroused tremendous interest from academia and industry, being regarded as an important class of photocatalysts. However, their photocatalytic activities still need further improvement to satisfy the requirement of scale-up production. In this regard, the surface engineering strategy is considered as one of the most effective methods for optimizing their photocatalytic performance. This feature article not only classifies the 2D photocatalysts into layered and non-layered 2D photocatalysts and presents their preferred synthesis methods, but also summarizes the advantages of the surface engineering strategy for boosting the photocatalytic performance of 2D materials from the aspects of light absorption, charge carrier separation and surface active sites. Various surface engineering strategies, such as surface decorating, vacancy engineering, element doping, surface heterojunction construction and regulation of facet-dependent sites, have also been presented as advantages of the surface engineering strategy. Eventually, the challenges and future outlook for optimizing the photocatalytic activities of 2D materials through surface engineering are addressed.", "gemini": "Được hưởng lợi từ cấu trúc độc đáo và tính chất lý hóa của chúng, các vật liệu hai chiều (2D) đã khơi dậy sự quan tâm to lớn từ học viện và ngành công nghiệp, được coi là một lớp chất xúc tác quang quan trọng. Tuy nhiên, hoạt động quang xúc tác của chúng vẫn cần được cải thiện thêm để đáp ứng yêu cầu sản xuất quy mô lớn. Về vấn đề này, chiến lược thiết kế bề mặt được coi là một trong những phương pháp hiệu quả nhất để tối ưu hóa hiệu suất quang xúc tác của chúng. Bài viết chuyên đề này không chỉ phân loại các chất xúc tác quang 2D thành chất xúc tác quang 2D nhiều lớp và không nhiều lớp và trình bày các phương pháp tổng hợp ưa thích của chúng, mà còn tóm tắt các ưu điểm của chiến lược thiết kế bề mặt để thúc đẩy hiệu suất quang xúc tác của vật liệu 2D từ các khía cạnh hấp thụ ánh sáng, tách hạt tải điện và các vị trí hoạt động bề mặt. Các chiến lược thiết kế bề mặt khác nhau, chẳng hạn như trang trí bề mặt, thiết kế chỗ trống, pha tạp nguyên tố, xây dựng dị hợp chất bề mặt và điều chỉnh các vị trí phụ thuộc vào mặt, cũng đã được trình bày như những ưu điểm của chiến lược thiết kế bề mặt. Cuối cùng, các thách thức và triển vọng trong tương lai để tối ưu hóa các hoạt động quang xúc tác của vật liệu 2D thông qua thiết kế bề mặt được giải quyết."} +{"idx": 1870492, "en": "Novel spintronic materials combining both magnetism and nontrivial topological electronic structures have attracted increasing attention recently. Here, we systematically studied the doping effects, magnetism, half-metallicity, and topological properties in the family of Fe2-xVxPO5 (x = 0, 0.5, 1, 1.5, 2) compounds. Our results show that Fe2PO5 takes an antiferromagnetic (AFM) ordering with a zero total magnetic moment. Meanwhile, the material hosts a Dirac nodal line and a Weyl nodal line near the Fermi level. V2PO5 is a ferromagnetic (FM) nodal line half-metal with a 100% spin-polarized Weyl nodal line. After doping, we find that Fe1.5V0.5PO5, Fe1V1PO5 and Fe0.5V1.5PO5 all take ferrimagnetic (FiM) ordering, with the Fe and V atoms taking opposite spin directions. Both Fe1.5V0.5PO5 and Fe0.5V1.5PO5 are FiM half-metals. Meanwhile, they show several pairs of fully spin-polarized Weyl points near the Fermi level. Fe1V1PO5 is a FiM semiconductor with different sizes of band gaps in different spin channels. These Fe2-xVxPO5 materials not only provide a good research platform to study the novel properties combining magnetism and nontrivial band topology, but also have promising applications in spintronic applications.", "gemini": "Các vật liệu spintronic mới kết hợp cả từ tính và cấu trúc điện tử tôpô phi tầm thường gần đây đã thu hút sự chú ý ngày càng tăng. Tại đây, chúng tôi đã nghiên cứu một cách có hệ thống các hiệu ứng tạp chất, từ tính, bán kim loại và các tính chất tôpô trong họ các hợp chất Fe2-xVxPO5 (x = 0, 0,5, 1, 1,5, 2). Kết quả của chúng tôi cho thấy Fe2PO5 có trật tự phản sắt từ (AFM) với mômen từ tổng bằng không. Trong khi đó, vật liệu chứa một đường nút Dirac và một đường nút Weyl gần mức Fermi. V2PO5 là một đường nút bán kim loại ferromagnét (FM) với một đường nút Weyl phân cực spin 100%. Sau khi tạp chất, chúng tôi thấy rằng Fe1,5V0,5PO5, Fe1V1PO5 và Fe0,5V1,5PO5 đều có trật tự ferrimagnetic (FiM), với các nguyên tử Fe và V có hướng spin ngược nhau. Cả Fe1,5V0,5PO5 và Fe0,5V1,5PO5 đều là các bán kim loại FiM. Trong khi đó, chúng cho thấy một số cặp điểm Weyl phân cực spin hoàn toàn gần mức Fermi. Fe1V1PO5 là một chất bán dẫn FiM với kích thước khác nhau của các khoảng trống dải trong các kênh spin khác nhau. Các vật liệu Fe2-xVxPO5 này không chỉ cung cấp một nền tảng nghiên cứu tốt để nghiên cứu các tính chất mới kết hợp từ tính và tôpô dải phi tầm thường, mà còn có các ứng dụng đầy hứa hẹn trong các ứng dụng spintronic."} +{"idx": 1870493, "en": "We report a new strongly birefringent material, [Al(H2O)6](IO3)2(NO3) (AINO). Based on a detailed analysis, we concluded that its enhanced birefringence (0.253 at 546 nm) predominantly resulted from a synergy of the effects of its (NO3)- and (IO3)- functional motifs, combined with an equally important and indispensable optimal arrangement of these two groups in the structure. This work has provided insight for discovering new birefringent materials.", "gemini": "Chúng tôi báo cáo một vật liệu lưỡng chiết mạnh mới, [Al(H2O)6](IO3)2(NO3) (AINO). Dựa trên phân tích chi tiết, chúng tôi kết luận rằng lưỡng chiết tăng cường của nó (0,253 ở 546 nm) chủ yếu là kết quả của sự hiệp đồng của các hiệu ứng của các họa tiết chức năng (NO3)- và (IO3)-, kết hợp với sự sắp xếp tối ưu quan trọng và không thể thiếu của hai nhóm này trong cấu trúc. Công trình này đã cung cấp cái nhìn sâu sắc để khám phá các vật liệu lưỡng chiết mới."} +{"idx": 1870494, "en": "Due to the extraordinary success of porphyrins in photodynamic therapy (PDT) and Ru compounds as chemotherapeutics, a series of RuII-porphyrin complexes have recently been synthesized and proposed as promising dual-action therapeutic agents. The results of a careful DFT and TDDFT investigation on four mononuclear pyridyl triphenylporphyrin RuII-arene complexes are herein reported and compared with those obtained for the metal-free derivatives. The investigation aims at shedding light on the modulation of the photophysical properties of the light absorber upon metalation and exploring the hydrolysis process of the RuII-moiety in the presence of the bulky porphyrin unit. Type I and Type II photoreactions were analyzed computing absorption spectra, singlet-triplet energy gaps, spin orbit coupling constants and vertical electron affinity (VEA) along with ionization potentials (VIP) for all the investigated compounds, while the chloride/water exchange reaction kinetics were determined by exploring the first and second aquation reactions of the Ru-moiety. Despite the highly similar photophysical properties displayed by the members of this class of compounds, an analysis of the hydrolysis processes in the dark allows to point out an interesting difference related to the type of pyridylporphyrin isomer and could be a preliminary explanation of the greater phototoxicity experimentally found for 3'-pyridyl substituted compounds.", "gemini": "Do thành công phi thường của porphyrin trong liệu pháp quang động (PDT) và các hợp chất Ru như thuốc hóa trị, một loạt các phức hợp RuII-porphyrin gần đây đã được tổng hợp và đề xuất như các tác nhân điều trị tác dụng kép đầy hứa hẹn. Kết quả của một cuộc điều tra DFT và TDDFT cẩn thận trên bốn phức hợp RuII-arene pyridyl triphenylporphyrin đơn nhân được báo cáo tại đây và so sánh với các kết quả thu được cho các dẫn xuất không chứa kim loại. Cuộc điều tra nhằm mục đích làm sáng tỏ sự điều chỉnh các tính chất quang vật lý của chất hấp thụ ánh sáng khi tạo kim loại và khám phá quá trình thủy phân của nhóm RuII khi có mặt đơn vị porphyrin cồng kềnh. Các phản ứng quang loại I và loại II đã được phân tích bằng cách tính toán phổ hấp thụ, khoảng cách năng lượng đơn ba, hằng số liên hợp quỹ đạo spin và ái lực điện tử thẳng đứng (VEA) cùng với thế ion hóa (VIP) cho tất cả các hợp chất được nghiên cứu, trong khi động học phản ứng trao đổi clorua/nước được xác định bằng cách khám phá các phản ứng ngậm nước đầu tiên và thứ hai của nhóm Ru. Mặc dù các tính chất quang vật lý rất giống nhau được thể hiện bởi các thành viên của lớp hợp chất này, nhưng việc phân tích các quá trình thủy phân trong bóng tối cho phép chỉ ra một sự khác biệt thú vị liên quan đến loại đồng phân pyridylporphyrin và có thể là lời giải thích sơ bộ về độc tính quang lớn hơn được tìm thấy trong các hợp chất thay thế 3'-pyridyl."} +{"idx": 1870495, "en": "Atomically precise gold nanoclusters protected by ligands are being intensely investigated in current catalysis science, due to the definitive correlation between the catalytic properties and structures at an atomic level. By solving the crystal structures of the nanoclusters, coupled with in situ and ex situ spectroscopy, a very fundamental understanding can be achieved to learn what controls the catalytic activation, active site structure, and catalytic mechanism. Herein, we mainly focus on the recent progress in catalysis controlled by precisely modulating the surface structures of the nanoclusters, including the alteration of the surface motifs, the doping of heterogeneous atoms in the surface of the nanoclusters, and the surface ligand engineering. The article is expected to help not only gain deep insight into the crucial roles of surface motifs of the nanoclusters in regulating the catalytic properties, but also explore the wide catalytic applications of atomically precise nanoclusters by elaborately tailoring the surface of the nanoclusters.", "gemini": "Các nano cụm vàng chính xác về mặt nguyên tử được bảo vệ bởi các phối tử đang được nghiên cứu sâu rộng trong khoa học xúc tác hiện đại, do có mối tương quan rõ ràng giữa các tính chất xúc tác và cấu trúc ở cấp độ nguyên tử. Bằng cách giải quyết các cấu trúc tinh thể của các nano cụm, kết hợp với phổ học tại chỗ và ngoài vị trí, có thể đạt được hiểu biết rất cơ bản để tìm hiểu những gì kiểm soát hoạt hóa xúc tác, cấu trúc vị trí hoạt động và cơ chế xúc tác. Trong bài báo này, chúng tôi chủ yếu tập trung vào những tiến bộ gần đây trong xúc tác được kiểm soát bằng cách điều chế chính xác các cấu trúc bề mặt của các nano cụm, bao gồm việc thay đổi các họa tiết bề mặt, việc đưa các nguyên tử không đồng nhất vào bề mặt của các nano cụm và kỹ thuật phối tử bề mặt. Bài báo này dự kiến sẽ không chỉ giúp hiểu sâu về vai trò quan trọng của các họa tiết bề mặt của các nano cụm trong việc điều chỉnh các tính chất xúc tác, mà còn khám phá các ứng dụng xúc tác rộng rãi của các nano cụm chính xác về mặt nguyên tử bằng cách thiết kế tỉ mỉ bề mặt của các nano cụm."} +{"idx": 1870496, "en": "Two-dimensional hydrogen boride (HB) sheets were recently demonstrated to act as a solid acid catalyst in their hydrogen-deficient state. However, both the active sites and the mechanism of the catalytic process require further elucidation. In this study, we analyzed the conversion of ethanol adsorbed on HB sheets under vacuum during heating using in situ Fourier transform infrared (FT-IR) absorption spectroscopy with isotope labelling. Up to 450 K, the FT-IR peak associated with the OH group of the adsorbed ethanol molecule disappeared from the spectrum, which was attributed to a dehydration reaction with a hydrogen atom from the HB sheet, resulting in the formation of an ethyl species. At temperatures above 440 K, the number of BD bonds markedly increased in CD3CH2OH, compared to CH3CD2OH; the temperature dependence of the formation rate of BD bonds was similar to that of the dehydration reaction rate of ethanol on HB sheets under steady-state conditions. The rate-determining step of the dehydration of ethanol on HB was thus ascribed to the dehydrogenation of the methyl group of the ethyl species on the HB sheets, followed by the immediate desorption of ethylene. These results show that the catalytic ethanol dehydration process on HB involves the hydrogen atoms of the HB sheets. The obtained mechanistic insights are expected to promote the practical application of HB sheets as catalysts.", "gemini": "Gần đây, các tấm borua hydro (HB) hai chiều đã được chứng minh là hoạt động như một chất xúc tác axit rắn ở trạng thái thiếu hydro của chúng. Tuy nhiên, cả các vị trí hoạt động và cơ chế của quá trình xúc tác đều cần được làm rõ thêm. Trong nghiên cứu này, chúng tôi đã phân tích quá trình chuyển đổi của etanol được hấp phụ trên các tấm HB trong chân không trong quá trình gia nhiệt bằng cách sử dụng phổ hấp thụ hồng ngoại biến đổi Fourier (FT-IR) tại chỗ với đánh dấu đồng vị. Lên đến 450 K, đỉnh FT-IR liên quan đến nhóm OH của phân tử etanol được hấp phụ biến mất khỏi quang phổ, điều này được quy cho phản ứng mất nước với một nguyên tử hydro từ tấm HB, dẫn đến sự hình thành của một loài etyl. Ở nhiệt độ trên 440 K, số liên kết BD tăng đáng kể trong CD3CH2OH, so với CH3CD2OH; sự phụ thuộc nhiệt độ của tốc độ hình thành liên kết BD tương tự như tốc độ phản ứng mất nước của etanol trên các tấm HB trong điều kiện trạng thái ổn định. Do đó, bước xác định tốc độ mất nước của etanol trên HB được quy cho phản ứng tách hydro của nhóm metyl của loài etyl trên các tấm HB, sau đó là sự giải hấp ngay lập tức của etylen. Những kết quả này cho thấy quá trình mất nước etanol xúc tác trên HB liên quan đến các nguyên tử hydro của các tấm HB. Những hiểu biết về cơ chế thu được dự kiến sẽ thúc đẩy ứng dụng thực tế của các tấm HB làm chất xúc tác."} +{"idx": 1870497, "en": "Indium selenide (InSe) photodetection devices attract significant research interest. However, InSe is unstable and degrades rapidly in ambient conditions, thus it is still a challenge to fabricate stable optoelectronic devices. In this work, multilayer InSe FETs are fabricated, and their photoresponse properties are investigated. Both positive and negative photoconductivities are observed for the first time in the same InSe FET in a wide spectral range from 450 nm to 660 nm, which can be tuned through changing either the gate bias or the source-drain bias. A physical mechanism is proposed to explain the dual-photoresponse phenomenon in our devices. Based on the proposed physical mechanism, as a proof of concept, a facile and simple approach is used to eliminate the negative photoconductivity of the InSe FET. Our results will offer valuable strategies for stable multilayer InSe optoelectronic device design, and a practical scheme for improving the performance of other transition metal dichalcogenide devices as well.", "gemini": "Các thiết bị quang điện inđi selenide (InSe) thu hút sự quan tâm nghiên cứu đáng kể. Tuy nhiên, InSe không ổn định và phân hủy nhanh chóng trong điều kiện môi trường, do đó, việc chế tạo các thiết bị quang điện tử ổn định vẫn là một thách thức. Trong công trình này, các FET InSe nhiều lớp được chế tạo và các tính chất phản ứng quang của chúng được nghiên cứu. Độ dẫn quang dương và âm lần đầu tiên được quan sát thấy trong cùng một FET InSe trong dải quang phổ rộng từ 450 nm đến 660 nm, có thể được điều chỉnh thông qua việc thay đổi điện áp cổng hoặc điện áp nguồn-kênh. Một cơ chế vật lý được đề xuất để giải thích hiện tượng phản ứng quang kép trong các thiết bị của chúng tôi. Dựa trên cơ chế vật lý được đề xuất, như một bằng chứng về khái niệm, một phương pháp dễ dàng và đơn giản được sử dụng để loại bỏ độ dẫn quang âm của FET InSe. Kết quả của chúng tôi sẽ cung cấp các chiến lược có giá trị cho thiết kế thiết bị quang điện tử InSe nhiều lớp ổn định và một kế hoạch thực tế để cải thiện hiệu suất của các thiết bị dichalcogenide kim loại chuyển tiếp khác."} +{"idx": 1870498, "en": "A new facile, rapid, stereo- and regio-selective one-pot synthesis of nucleoside-2',3'-O,O-phosphorothioate and selenoate analogs has been developed. This method avoids the need for protection strategies and chiral reagents, chiral metal catalysts, or chiral separations. This synthetic method has been applied to all natural nucleosides (U/A/G/C/T). Furthermore, we have deciphered the origin of the stereo- and regio-selectivity of the reaction.", "gemini": "Một phương pháp tổng hợp một nồi phản ứng mới dễ dàng, nhanh chóng, chọn lọc lập thể và vùng của các chất tương tự nucleoside-2 ', 3'-O, O-phosphothioate và selenoate đã được phát triển. Phương pháp này tránh được nhu cầu về các chiến lược bảo vệ và thuốc thử quang học, chất xúc tác kim loại quang học hoặc tách quang học. Phương pháp tổng hợp này đã được áp dụng cho tất cả các nucleoside tự nhiên (U / A / G / C / T). Hơn nữa, chúng tôi đã giải mã nguồn gốc của tính chọn lọc lập thể và vùng của phản ứng."} +{"idx": 1870499, "en": "Four coordination polymers (CPs) Mn-TMPP (1), Zn-TMPP (2), Mn-THPP (3), and Zn-THPP (4) have been synthesized and characterized (H2TMPP = meso-tetrakis (6-methylpyridin-3-yl) porphyrin; H2THPP = meso-tetrakis (6-(hydroxymethyl) pyridin-3-yl) porphyrin). The one-dimensional (1D) chain compound 1 is formed via a head-to-tail connection of the Mn-TMPP unit, wherein the central Mn2+ features a square pyramidal geometry coordinated by four N atoms from the porphyrin skeleton and one additional N atom from an adjacent Mn-TMPP unit. Compound 2 features an octahedral Zn2+ center associated with four N atoms from the porphyrin skeleton to define the equatorial plane and two additional N donors at the axial positions to give a two-dimensional (2D) CP. The 1D chain of 1 and the 2D layer of 2 possess distinctive molecular structures but nearly identical molecular arrangements in their unit cells viewed along all three crystallographic axes. By contrast, Mn- and Zn-based CPs 3 and 4 supported by the THPP ligand share both identical molecular connectivities and crystal packing. In 3/4, each Mn/Zn center is chelated by four N donors of the porphyrin interior to define the equatorial plane of an octahedron, whose axial sites are occupied by two alcoholic OH groups from a pair of trans-located pyridinemethanol moieties. The third-order nonlinear optical properties of 1-4 investigated using the Z-scan technique at 532 nm revealed reverse saturable absorption and self-focusing effects for all four CPs, with hyperpolarizability values (γ) in the range 1.42 × 10-28 esu to 7.64 × 10-28 esu. These high γ values are comparable to the best porphyrin-based molecular assemblies, demonstrating potential for these materials in optical limiting applications.", "gemini": "Bốn polyme phối hợp (CP) Mn-TMPP (1), Zn-TMPP (2), Mn-THPP (3) và Zn-THPP (4) đã được tổng hợp và đặc trưng (H2TMPP = meso-tetrakis (6-methylpyridin-3-yl) porphyrin; H2THPP = meso-tetrakis (6- (hydroxymethyl) pyridin-3-yl) porphyrin). Hợp chất chuỗi một chiều (1D) 1 được hình thành thông qua kết nối đầu đuôi của đơn vị Mn-TMPP, trong đó Mn2 + trung tâm có hình học hình chóp vuông được phối hợp bởi bốn nguyên tử N từ bộ khung porphyrin và một nguyên tử N bổ sung từ một đơn vị Mn-TMPP liền kề. Hợp chất 2 có một trung tâm Zn2 + hình bát diện liên kết với bốn nguyên tử N từ bộ khung porphyrin để xác định mặt phẳng xích đạo và hai nhóm cho N bổ sung ở các vị trí trục để tạo thành một CP hai chiều (2D). Chuỗi 1D của 1 và lớp 2D của 2 sở hữu các cấu trúc phân tử khác biệt nhưng có sự sắp xếp phân tử gần như giống hệt nhau trong các ô đơn vị của chúng khi quan sát dọc theo cả ba trục tinh thể học. Ngược lại, CP dựa trên Mn và Zn 3 và 4 được hỗ trợ bởi phối tử THPP có chung cả kết nối phân tử và đóng gói tinh thể giống hệt nhau. Trong 3/4, mỗi trung tâm Mn / Zn được liên kết bởi bốn nhóm cho N của phần bên trong porphyrin để xác định mặt phẳng xích đạo của một bát diện, các vị trí trục của nó được chiếm giữ bởi hai nhóm OH có cồn từ một cặp nhóm pyridinemethanol được chuyển vị. Các tính chất quang học phi tuyến bậc ba của 1-4 được điều tra bằng kỹ thuật quét Z ở bước sóng 532 nm cho thấy sự hấp thụ bão hòa ngược và hiệu ứng tự hội tụ đối với cả bốn CP, với các giá trị siêu phân cực (γ) trong khoảng 1,42 × 10-28 esu đến 7,64 × 10-28 esu. Các giá trị γ cao này tương đương với các hợp chất phân tử dựa trên porphyrin tốt nhất, chứng tỏ tiềm năng của các vật liệu này trong các ứng dụng giới hạn quang."} +{"idx": 1870500, "en": "Asymmetric synthesis based on DNA scaffolds has been actively exploited because of the advantages of DNA such as diverse tertiary structures, chemical stability, and easy handling. Since duplex DNA-based hybrid catalysts have demonstrated this remarkable capability, efforts have been made to investigate new biomolecular scaffolds. Herein, we report modular quadruplex-duplex (QD) hybrid DNA catalysts containing bipyridine ligands and hydrogen donor moieties. The conformation, thermal stability, and metal-binding ability of modified QD hybrid DNA were characterized using spectroscopy. The QD hybrid-based DNA catalysts were successfully applied to asymmetric Michael addition reactions (86% conversion and 76% ee). This study describes a new type of DNA hybrid catalyst produced by the construction of a cooperative active site with a Lewis acid and a H-bond donor.", "gemini": "Tổng hợp không đối xứng dựa trên khung DNA đã được khai thác tích cực vì những ưu điểm của DNA như cấu trúc bậc ba đa dạng, tính ổn định về mặt hóa học và dễ xử lý. Vì các chất xúc tác lai dựa trên DNA duplex đã chứng minh khả năng đáng chú ý này, nên những nỗ lực đã được thực hiện để nghiên cứu các khung sinh học phân tử mới. Trong bài báo này, chúng tôi báo cáo các chất xúc tác DNA lai bốn chuỗi tứ phân-song công (QD) chứa các phối tử bipyridine và nhóm cho hydro. Cấu hình, độ ổn định nhiệt và khả năng liên kết kim loại của DNA lai QD đã được sửa đổi được đặc trưng bằng quang phổ học. Các chất xúc tác DNA dựa trên lai QD đã được ứng dụng thành công vào các phản ứng cộng Michael không đối xứng (chuyển đổi 86% và ee 76%). Nghiên cứu này mô tả một loại chất xúc tác lai DNA mới được tạo ra bằng cách xây dựng một vị trí hoạt động hợp tác với axit Lewis và chất cho liên kết H."} +{"idx": 1870501, "en": "Single-atom catalysts (SACs) with atomically dispersed metals have emerged as a new class of heterogeneous catalysts and have attracted considerable interest because they offer 100% metal atom utilization and show excellent catalytic behavior compared with traditionally supported nano-particles. However, it is challenging to explore the active sites and catalytic mechanisms of SACs through common characterization methods due to the isolated single atoms. Therefore, employing theoretical calculations to determine the nature of SACs' active sites and the reaction mechanisms is particularly meaningful. This paper describes the nature of SACs by summarizing the diverse applications and properties of SACs, which starts from computational simulation on a couple of important applications of SACs. Then the distinctive and fundamental properties of SACs are discussed. At last, the challenges and future perspectives of computational calculations for SACs are outlined.", "gemini": "Các chất xúc tác đơn nguyên tử (SAC) với các kim loại phân tán nguyên tử đã nổi lên như một lớp chất xúc tác không đồng nhất mới và đã thu hút được sự quan tâm đáng kể vì chúng cung cấp 100% khả năng sử dụng nguyên tử kim loại và thể hiện hành vi xúc tác tuyệt vời so với các hạt nano được hỗ trợ theo cách truyền thống. Tuy nhiên, việc khám phá các vị trí hoạt động và cơ chế xúc tác của SAC thông qua các phương pháp đặc trưng thông thường là một thách thức do các nguyên tử đơn lẻ bị cô lập. Do đó, việc sử dụng các tính toán lý thuyết để xác định bản chất của các vị trí hoạt động của SAC và cơ chế phản ứng có ý nghĩa đặc biệt. Bài báo này mô tả bản chất của SAC bằng cách tóm tắt các ứng dụng và đặc tính đa dạng của SAC, bắt đầu từ mô phỏng tính toán trên một số ứng dụng quan trọng của SAC. Sau đó, các đặc tính đặc biệt và cơ bản của SAC được thảo luận. Cuối cùng, các thách thức và triển vọng trong tương lai của các phép tính tính toán cho SAC được nêu ra."} +{"idx": 1870502, "en": "Multivalency is nature's way to establish firm and specific interactions when the binding sites of a protein receptor have only low affinity for monovalent ligands. Recently, researchers are increasingly using nucleic acid architectures for multivalent ligand presentation to unravel the mechanisms of multivalency-enhanced interactions and create high affinity binding agents. In contrast to other polymers, nucleic acid materials are capable of accessing a wide variety of rigid three-dimensional structures through the sequence-programed self-assembly of component strands. By controlling the number of ligands and their distances, researchers can construct tailor-made probes for interrogating multivalent interactions with Ångstrom precision. Nucleic acid assemblies have been used to address fundamental questions of multivalency in order to unravel how monovalent interaction strength, scaffold flexibility, distances between interacting sites and spatial arrangement influence the achievable affinity gains. In a slightly different approach, nucleic acid constructs have been applied as chemical dimerizers of protein receptors, to investigate the importance of receptor proximity or construct tools that provide control over biological signal transduction processes. In this review, we discuss multivalent nucleic acid-ligand conjugates in the context of the biological protein receptors they interrogate. We recount pioneering work and seminal studies performed within the last 10 years describing the in vitro interrogation of proteins recognizing carbohydrate ligands, small molecules, peptides and nucleic acid aptamers and we portray work performed with viruses, cell models, and whole organisms.", "gemini": "Đa hóa trị là cách tự nhiên để thiết lập các tương tác chắc chắn và cụ thể khi các vị trí liên kết của thụ thể protein chỉ có ái lực thấp đối với các phối tử đơn hóa trị. Gần đây, các nhà nghiên cứu ngày càng sử dụng kiến trúc axit nucleic để trình bày phối tử đa hóa trị nhằm làm sáng tỏ các cơ chế tương tác tăng cường đa hóa trị và tạo ra các chất liên kết có ái lực cao. Trái ngược với các polyme khác, vật liệu axit nucleic có khả năng tiếp cận nhiều cấu trúc ba chiều cứng thông qua quá trình tự lắp ráp theo trình tự của các sợi thành phần. Bằng cách kiểm soát số lượng phối tử và khoảng cách của chúng, các nhà nghiên cứu có thể xây dựng các đầu dò được thiết kế riêng để thẩm vấn các tương tác đa hóa trị với độ chính xác Ångstrom. Các hợp chất axit nucleic đã được sử dụng để giải quyết các câu hỏi cơ bản về đa hóa trị nhằm làm sáng tỏ cách cường độ tương tác đơn hóa trị, tính linh hoạt của khung, khoảng cách giữa các vị trí tương tác và sự sắp xếp không gian ảnh hưởng đến mức độ ái lực đạt được. Trong một cách tiếp cận hơi khác, các cấu trúc axit nucleic đã được áp dụng làm chất đồng trùng hợp hóa học của các thụ thể protein, để nghiên cứu tầm quan trọng của sự gần gũi của thụ thể hoặc xây dựng các công cụ cung cấp khả năng kiểm soát các quá trình truyền tín hiệu sinh học. Trong bài đánh giá này, chúng tôi thảo luận về các liên hợp axit nucleic-phối tử đa hóa trị trong bối cảnh các thụ thể protein sinh học mà chúng thẩm vấn. Chúng tôi kể lại công trình tiên phong và các nghiên cứu quan trọng được thực hiện trong 10 năm qua để mô tả quá trình thẩm vấn trong ống nghiệm các protein nhận ra các phối tử carbohydrate, các phân tử nhỏ, các peptide và các aptme axit nucleic và chúng tôi mô tả công trình được thực hiện với vi-rút, mô hình tế bào và toàn bộ sinh vật."} +{"idx": 1870503, "en": "Herein, two kinds of vertical organic optoelectronic devices, vertical organic field-effect transistors (VOFETs) and light-emitting transistors (VOLETs), were constructed based on amorphous organic semiconductors of N,N'-di(1-naphthyl)-N,N'-diphenyl-(1,1'-biphenyl)-4,4'-diamine (NPB) as hole injecting and transport layers and tris(8-hydroxy-quinolinato) aluminum (Alq3) as the emitting layer. High device performances with a large on/off ratio of ∼6 × 103, current density of ∼40 mA cm-2, and fast response of ∼5 ms at a frequency of 20 Hz and a brightness of 126 cd m-2 were demonstrated for these two vertical devices with good device stability and repeatability. These results suggest the potential applications of amorphous organic semiconductors with good film-forming characteristics and easy device fabrication ability in vertical optoelectronic circuits.", "gemini": "Ở đây, hai loại thiết bị quang điện tử hữu cơ theo chiều dọc, tranzito hiệu ứng trường hữu cơ theo chiều dọc (VOFET) và tranzito phát sáng (VOLET), đã được chế tạo dựa trên chất bán dẫn hữu cơ vô định hình của N,N'-di(1-naphthyl)-N,N'-diphenyl-(1,1'-biphenyl)-4,4'-diamine (NPB) làm lớp tiêm và vận chuyển lỗ và tris(8-hydroxy-quinolinato) nhôm (Alq3) làm lớp phát sáng. Hiệu suất thiết bị cao với tỷ lệ bật/tắt lớn ∼6 × 103, mật độ dòng điện ∼40 mA cm-2 và phản hồi nhanh ∼5 ms ở tần số 20 Hz và độ sáng 126 cd m-2 đã được chứng minh cho hai thiết bị dọc này với độ ổn định và khả năng lặp lại của thiết bị tốt. Những kết quả này cho thấy các ứng dụng tiềm năng của chất bán dẫn hữu cơ vô định hình với đặc tính tạo màng tốt và khả năng chế tạo thiết bị dễ dàng trong các mạch quang điện tử theo chiều dọc."} +{"idx": 1870504, "en": "Ruthenium(ii) diclofenac-based complexes of the general formula [Ru(dicl)(P-P)(bpy)]PF6 [dicl = diclofenac, bpy = 2,2'-bipyridine, and P-P = 1,4'-bis(diphenylphosphino)butane (dppb) (1), 1,2'-bis(diphenylphosphino)ethane (dppe) (2), 1,3'-bis(diphenylphosphino)propane (dppp) (3) and 1,1'-bis(diphenylphosphino)ferrocene (dppf) (4)] are synthesized. The complexes (1-4) are characterized by elemental analyses, infrared, NMR, and UV-vis spectroscopy and (3) and (4) are characterized by single crystal X-ray diffraction. The DNA binding of complexes (1-4), studied by circular dichroism (CD) and Hoechst 33 258 staining assay, indicates their binding with the minor grooves. The complexes interact with BSA with binding constants (Kb) in the range of 2.5 × 103-5.5 × 104 M-1. The complexes exhibit high cytotoxicity against the tumor cell lines A549, MDA-MB-231, and MCF-7 with IC50 values ranging from 0.56 to 15.28 μM. The complexes are more selective for the hormone-dependent MCF-7 breast tumor cell line and complex (1) is the most potent one. The study demonstrates the anticancer activity of ruthenium(ii)/diclofenac-based complexes.", "gemini": "Các phức hợp ruthenium(ii) diclofenac dựa trên công thức chung [Ru(dicl)(P-P)(bpy)]PF6 [dicl = diclofenac, bpy = 2,2'-bipyridine, và P-P = 1,4'-bis(diphenylphosphino) butan (dppb) (1), 1,2'-bis(diphenylphosphino) ethan (dppe) (2), 1,3'-bis(diphenylphosphino) propan (dppp) (3) và 1,1'-bis(diphenylphosphino) ferocen (dppf) (4)] được tổng hợp. Các phức hợp (1-4) được đặc trưng bởi các phép phân tích nguyên tố, hồng ngoại, NMR và quang phổ UV-vis và (3) và (4) được đặc trưng bằng nhiễu xạ tia X tinh thể đơn. Liên kết DNA của các phức hợp (1-4), được nghiên cứu bằng phương pháp sắc ký tròn hai cực (CD) và phương pháp nhuộm Hoechst 33 258, cho thấy liên kết của chúng với các rãnh nhỏ. Các phức hợp tương tác với BSA với hằng số liên kết (Kb) trong khoảng 2,5 × 103-5,5 × 104 M-1. Các phức hợp thể hiện độc tính tế bào cao đối với các dòng tế bào khối u A549, MDA-MB-231 và MCF-7 với giá trị IC50 trong khoảng từ 0,56 đến 15,28 μM. Các phức hợp có tính chọn lọc cao hơn đối với dòng tế bào khối u vú MCF-7 phụ thuộc hormone và phức hợp (1) là phức hợp mạnh nhất. Nghiên cứu chứng minh hoạt tính chống ung thư của các phức hợp dựa trên ruthenium(ii)/diclofenac."} +{"idx": 1870505, "en": "Carnosic acid (CA) represents one of the most effective antioxidants that can be applied for the prevention of degenerative and chronic diseases. However, the intrinsic hydrophobic nature of CA results in low solubility and poor dissolution in the gastrointestinal (GI) tract, which limits its applications in a variety of functional food systems. In order to address these issues, we encapsulated CA in a lecithin-based nanoemulsion (CA-NE) to improve its bioaccessibility and bioavailability which are evaluated using in vitro and in vivo digestion models. The CA-NE demonstrated a loading capacity of 2.6-3.0%, an average particle size of 165 nm, a ζ-potential value of -57.2 mV, and good stability during 4-weeks of storage at 4, 25, and 37 °C. The in vitro static pH-stat lipolysis model and dynamic TNO gastrointestinal (TIM-1) model demonstrated a 12.6 and 5.6 fold increase in the total bioaccessibility of CA encapsulated in nanoemulsion, respectively, as opposed to CA in suspension form. Moreover, the in vivo pharmacokinetics study on a rat model (Male Sprague Dawley) confirmed that the bioavailability of CA in nanoemulsion showed a 2.2 fold increase, as compared to that of CA in suspension form. In conclusion, the bioaccessibility and bioavailability of CA were remarkably improved by encapsulation of CA in a lecithin-based nanoemulsion. Moreover, the combined in vitro and in vivo study could serve as a useful approach for the comprehensive evaluation of oral lipid-based delivery systems.", "gemini": "Axit carnosic (CA) là một trong những chất chống oxy hóa hiệu quả nhất có thể được áp dụng để phòng ngừa các bệnh thoái hóa và mãn tính. Tuy nhiên, bản chất kỵ nước vốn có của CA dẫn đến độ hòa tan thấp và khả năng hòa tan kém trong đường tiêu hóa (GI), điều này hạn chế các ứng dụng của nó trong nhiều hệ thống thực phẩm chức năng. Để giải quyết những vấn đề này, chúng tôi đã đóng gói CA trong một nano nhũ tương dựa trên lecithin (CA-NE) để cải thiện khả năng sinh học và khả dụng sinh học của nó được đánh giá bằng các mô hình tiêu hóa trong ống nghiệm và trong cơ thể sống. CA-NE chứng minh khả năng tải 2,6-3,0%, kích thước hạt trung bình 165 nm, giá trị ζ-điện thế là -57,2 mV và độ ổn định tốt trong 4 tuần bảo quản ở 4, 25 và 37 °C. Mô hình thủy phân lipolysis pH tĩnh trong ống nghiệm và mô hình tiêu hóa TNO động (TIM-1) chứng minh sự gia tăng gấp 12,6 và 5,6 lần về khả dụng sinh học tổng thể của CA được đóng gói trong nano nhũ tương, trái ngược với CA ở dạng huyền phù. Hơn nữa, nghiên cứu dược động học trong cơ thể sống trên mô hình chuột (Chuột Sprague Dawley đực) xác nhận rằng khả dụng sinh học của CA trong nano nhũ tương tăng gấp 2,2 lần so với CA ở dạng huyền phù. Tóm lại, khả năng sinh học và khả dụng sinh học của CA được cải thiện đáng kể bằng cách đóng gói CA trong nano nhũ tương dựa trên lecithin. Hơn nữa, nghiên cứu kết hợp trong ống nghiệm và trong cơ thể sống có thể đóng vai trò là một phương pháp hữu ích để đánh giá toàn diện các hệ thống phân phối dựa trên lipid đường uống."} +{"idx": 1870506, "en": "Nanostructured manganese dioxide (MnO2) has attracted extensive attention in the field of anticancer applications. As we all know, the tumor microenvironment is usually characterized by a high glutathione (GSH) concentration, overproduced hydrogen peroxide (H2O2), acidity, and hypoxia, which affect the efficacy of many traditional treatments such as chemotherapy, radiotherapy, and surgery. Fortunately, as one kind of redox-active nanomaterial, nanostructured MnO2 has many excellent properties such as strong oxidation ability, excellent catalytic activity, and good biodegradability. It can be used effectively in diagnosis and treatment when it reacts with some harmful substances in the tumor site. It can not only enhance the therapeutic effect but also adjust the tumor microenvironment. Therefore, it is necessary to present the recent achievements and progression of nanostructured MnO2 for anticancer applications, including preparation methods, diagnosis, and treatment. Special attention was paid to photodynamic therapy (PDT), bioimaging and cancer diagnosis (BCD), and drug delivery systems (DDS). This review is expected to provide helpful guidance on further research of nanostructured MnO2 for anticancer applications.", "gemini": "Điôxit mangan có cấu trúc nano (MnO2) đã thu hút sự chú ý rộng rãi trong lĩnh vực ứng dụng chống ung thư. Như chúng ta đã biết, môi trường vi mô của khối u thường được đặc trưng bởi nồng độ glutathione (GSH) cao, hydro peroxide (H2O2) được sản xuất quá mức, tính axit và tình trạng thiếu oxy, ảnh hưởng đến hiệu quả của nhiều phương pháp điều trị truyền thống như hóa trị, xạ trị và phẫu thuật. May mắn thay, là một loại vật liệu nano hoạt động oxy hóa khử, MnO2 có cấu trúc nano có nhiều đặc tính tuyệt vời như khả năng oxy hóa mạnh, hoạt tính xúc tác tuyệt vời và khả năng phân hủy sinh học tốt. Nó có thể được sử dụng hiệu quả trong chẩn đoán và điều trị khi phản ứng với một số chất có hại tại vị trí khối u. Nó không chỉ có thể tăng cường hiệu quả điều trị mà còn điều chỉnh môi trường vi mô của khối u. Do đó, cần trình bày những thành tựu và tiến triển gần đây của MnO2 có cấu trúc nano cho các ứng dụng chống ung thư, bao gồm các phương pháp chế tạo, chẩn đoán và điều trị. Sự chú ý đặc biệt được dành cho liệu pháp quang động (PDT), chẩn đoán hình ảnh sinh học và ung thư (BCD) và hệ thống phân phối thuốc (DDS). Đánh giá này dự kiến sẽ cung cấp hướng dẫn hữu ích cho nghiên cứu sâu hơn về MnO2 có cấu trúc nano cho các ứng dụng chống ung thư."} +{"idx": 1870507, "en": "Inspired by the structural coloring in nature, especially the crystalline ordering and responsive characteristics of those found in chameleon skins, artificial photonic materials for sensor applications were fabricated. Cholesteric liquid crystals (CLCs) were employed in the templated synthesis of polymeric particles with periodic structures that allow visible light to undergo Bragg reflection and their response was tested against volatile organic compounds (VOCs). We demonstrate that the particles were responsive against toluene with detection limits on the order of 100 ppm. Such sensitivity was shown to be achieved due to the critical steps followed during the CLC-templated synthesis of particles that resulted in the storage of elastic energy in the anisotropic glassy polymer network. In addition, the design of particle-assisted sensor chips that allow easy integration into wearable optical devices for reliable, continuous and online tracking of VOC concentrations is presented. These results proved that sensors developed from the CLC-templated particles can be used multiple times without a significant loss of sensitivity and offered rapid, sensitive and battery-free detection.", "gemini": "Lấy cảm hứng từ màu sắc cấu trúc trong tự nhiên, đặc biệt là trật tự tinh thể và các đặc điểm phản ứng của những thứ được tìm thấy trong da tắc kè, vật liệu quang tử nhân tạo cho các ứng dụng cảm biến đã được chế tạo. Tinh thể lỏng Cholesteric (CLC) được sử dụng trong quá trình tổng hợp có khuôn mẫu các hạt polyme với cấu trúc tuần hoàn cho phép ánh sáng khả kiến phản xạ Bragg và phản ứng của chúng đã được thử nghiệm với các hợp chất hữu cơ dễ bay hơi (VOC). Chúng tôi chứng minh rằng các hạt phản ứng với toluene với giới hạn phát hiện vào khoảng 100 ppm. Độ nhạy như vậy đã được chứng minh là đạt được do các bước quan trọng được thực hiện trong quá trình tổng hợp các hạt có khuôn mẫu CLC dẫn đến việc lưu trữ năng lượng đàn hồi trong mạng lưới polyme thủy tinh dị hướng. Ngoài ra, thiết kế chip cảm biến hỗ trợ hạt cho phép tích hợp dễ dàng vào các thiết bị quang học đeo được để theo dõi nồng độ VOC đáng tin cậy, liên tục và trực tuyến. Những kết quả này chứng minh rằng các cảm biến được phát triển từ các hạt có khuôn mẫu CLC có thể được sử dụng nhiều lần mà không bị mất độ nhạy đáng kể và cung cấp khả năng phát hiện nhanh, nhạy và không cần pin."} +{"idx": 1870508, "en": "Employing TBN/TEMPO as the catalysts and oxygen as the oxidant, the biologically and pharmaceutically significant tetrahydro-β-carboline and β-carboline alkaloid scaffolds that used to be obtained by multi-step processes can now be selectively obtained in only one-step via direct aerobic oxidative Pictet-Spengler reactions of tryptamines with alcohols under mild conditions, with water generated as the byproduct. In this reaction, TBN/TEMPO was interestingly found to be able to facilitate the cyclization step of the whole reaction. This method tolerates a variety of C- and N-substituted tryptamines, and both the more reactive benzylic and allylic alcohols and the less reactive aliphatic alcohols. This method can also be extended to dihydro-β-carboline synthesis and applied to the more available and more economical tryptophan for β-carboline synthesis, revealing its broad substrate scope and potential in synthetic applications.", "gemini": "Sử dụng TBN/TEMPO làm chất xúc tác và oxy làm chất oxy hóa, các khung tetrahydro-β-carboline và alkaloid β-carboline có ý nghĩa sinh học và dược lý đáng kể trước đây được thu được bằng các quy trình nhiều bước hiện có thể được thu được một cách chọn lọc chỉ trong một bước thông qua phản ứng Pictet-Spengler oxy hóa hiếu khí trực tiếp của tryptamine với rượu trong điều kiện nhẹ, với nước được tạo ra như sản phẩm phụ. Trong phản ứng này, người ta thấy rằng TBN/TEMPO có thể tạo điều kiện cho bước tạo vòng của toàn bộ phản ứng. Phương pháp này dung nạp nhiều loại tryptamine thế C và N, và cả rượu benzylic và rượu allylic phản ứng mạnh hơn và rượu aliphatic phản ứng kém hơn. Phương pháp này cũng có thể được mở rộng để tổng hợp dihydro-β-carboline và áp dụng cho tryptophan có sẵn và kinh tế hơn để tổng hợp β-carboline, cho thấy phạm vi chất nền rộng và tiềm năng của nó trong các ứng dụng tổng hợp."} +{"idx": 1870509, "en": "Owing to a finite and single-atom-thick two-dimensional structure, graphene nanostructures such as nanoribbons possess outstanding physical properties and unique size-dependent characteristics due to nanoscale defects, especially for mechanical properties. Graphene nanostructures characteristically exhibit strong nonlinearity in deformation and the defect brings about an extremely localized singular stress field of only a few nanometers, which might lead to unique fracture properties. Fundamental understanding of their fracture properties and criteria is, however, seriously underdeveloped and limited to the level of continuum mechanics and linear elasticity. Here, we demonstrate the breakdown of continuum-based fracture criteria for graphene nanoribbons due to the strong nonlinearity and discreteness of atoms emerging with decreasing size and identify the critical sizes for these conventional criteria. We further propose an energy-based criterion considering atomic discrete nature, and show that it can successfully describe the fracture beyond the critical sizes. The complete clarification of fracture criterion for nonlinear graphene with nanoscale singularity contributes not only to the reliable design of graphene-based nanodevices but also to the elucidation of the extreme dimensional limit in fracture mechanics.", "gemini": "Do cấu trúc hai chiều mỏng như nguyên tử và hữu hạn, các nanocấu trúc graphene như nanoribbon sở hữu các tính chất vật lý vượt trội và các đặc tính độc đáo phụ thuộc vào kích thước do các khuyết tật ở cấp độ nano, đặc biệt là đối với các tính chất cơ học. Các nanocấu trúc graphene đặc trưng là thể hiện tính phi tuyến tính mạnh trong biến dạng và khuyết tật tạo ra một trường ứng suất kỳ dị cực kỳ cục bộ chỉ vài nanomet, có thể dẫn đến các tính chất gãy nứt độc đáo. Tuy nhiên, sự hiểu biết cơ bản về các tính chất gãy nứt và tiêu chí của chúng vẫn còn rất kém phát triển và chỉ giới hạn ở mức độ cơ học liên tục và độ đàn hồi tuyến tính. Ở đây, chúng tôi chứng minh sự phá vỡ các tiêu chí gãy dựa trên liên tục đối với nanoribbon graphene do tính phi tuyến tính mạnh và tính rời rạc của các nguyên tử xuất hiện khi kích thước giảm dần và xác định các kích thước tới hạn cho các tiêu chí thông thường này. Hơn nữa, chúng tôi đề xuất một tiêu chuẩn dựa trên năng lượng, xem xét bản chất rời rạc của nguyên tử và chỉ ra rằng nó có thể mô tả thành công sự gãy nứt vượt quá các kích thước tới hạn. Việc làm rõ hoàn toàn tiêu chuẩn gãy nứt cho graphene phi tuyến tính với kỳ dị ở cấp độ nano không chỉ góp phần vào thiết kế đáng tin cậy các thiết bị nano dựa trên graphene mà còn làm sáng tỏ giới hạn kích thước cực đại trong cơ học gãy nứt."} +{"idx": 1870510, "en": "Hexagonal boron nitride is well known for its unique structure and excellent physical properties, particularly in hexagonal boron nitride nanosheets (BNNSs) with high potential in multiple technological applications. However, its severe layer-by-layer aggregation and incompatibility with processing liquids or condensed phase materials pose a great challenge. Covalent functionalization of BNNSs has been a common approach to address these critical issues, yet it is extremely difficult to carry out due to the chemical inertness of BNNSs. In this study, we report a novel and general route to covalently functionalize BNNSs via a simple reduction reaction. This involves initial negative charging through effective reductive activation which enables subsequent reactions with various organic alkyl halides. Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA) results confirm that linear alkyl chains with varying lengths are successfully grafted onto BNNSs, which leads to matched compatibility with organic media and the exfoliation level of few-layer thickness. The increase of the alkyl chain length considerably promotes their solubility in organic solvents with iodoalkanes as the most efficient grafting agents. Incorporation of alkylated BNNSs into a polymer matrix at low filler loadings leads to significant enhancements in mechanical properties over neat polymers, suggesting their exceptional reinforcement for polymer nanocomposites. This facile and scalable reductive chemistry route is applicable to versatile chemical modifications of BNNSs with diverse functional groups and grafting agents by reactions with suitable electrophiles.", "gemini": "Hexagonal boron nitride được biết đến với cấu trúc độc đáo và các đặc tính vật lý tuyệt vời, đặc biệt là trong các tấm nano hexagonal boron nitride (BNNS) có tiềm năng cao trong nhiều ứng dụng công nghệ. Tuy nhiên, sự kết tụ từng lớp nghiêm trọng và không tương thích với các chất lỏng chế biến hoặc vật liệu pha ngưng tụ của nó đặt ra một thách thức lớn. Chức năng hóa cộng hóa trị của BNNS là một cách tiếp cận phổ biến để giải quyết các vấn đề quan trọng này, nhưng lại cực kỳ khó thực hiện do tính trơ về mặt hóa học của BNNS. Trong nghiên cứu này, chúng tôi báo cáo một lộ trình mới và chung để chức năng hóa cộng hóa trị BNNS thông qua phản ứng khử đơn giản. Điều này liên quan đến việc tích điện âm ban đầu thông qua quá trình hoạt hóa khử hiệu quả cho phép các phản ứng tiếp theo với nhiều loại alkyl halide hữu cơ. Phổ hồng ngoại biến đổi Fourier (FTIR), phổ điện tử quang X (XPS) và kết quả phân tích nhiệt trọng lượng (TGA) xác nhận rằng các chuỗi alkyl tuyến tính với độ dài khác nhau được ghép thành công vào BNNS, dẫn đến khả năng tương thích phù hợp với môi trường hữu cơ và mức độ bong tróc của độ dày vài lớp. Sự gia tăng chiều dài chuỗi alkyl thúc đẩy đáng kể khả năng hòa tan của chúng trong dung môi hữu cơ với iodoalkan là chất ghép hiệu quả nhất. Việc kết hợp BNNS alkyl hóa vào ma trận polyme ở mức độ tải chất độn thấp dẫn đến sự gia tăng đáng kể về các tính chất cơ học so với polyme nguyên chất, cho thấy khả năng gia cường đặc biệt của chúng đối với polyme nanocomposite. Lộ trình hóa học khử dễ dàng và có thể mở rộng này có thể áp dụng cho các sửa đổi hóa học linh hoạt của BNNS với nhiều nhóm chức năng và chất ghép khác nhau bằng phản ứng với các chất ái điện tử phù hợp."} +{"idx": 1870511, "en": "Cross-species hybridizations have been extensively used to generate animals and plants better suited for draft and food and fiber production since Roman times, and are still important in current agricultural practices with growing uses especially in aquaculture. Diagnostic tools based on marker panels with sufficient numbers of markers for accurate identification of cross-species hybrid individuals from intercrossed and backcrossed populations are increasingly necessary for practical, accurate species-purity certification and management of commercial broodstocks. Minimal numbers of di-allelic markers with species-specific alleles required to accurately identify hybrid individuals in intercrossed and advanced backcrossed populations were estimated using power analysis, and ranged from 5 to 191 (α = .05), and from 7 to 293 (α = .01), considering backcross 1 (BC1) to BC6 populations, respectively. Numbers of markers required for accurate hybrid identification observed in simulated BC1 to BC6 populations ranged from 5 to 1,131 and 7 to 8,065, considering error rates ≤ 5% and ≤ 1%, respectively. Estimated and observed numbers of diagnostic markers required for accurate hybrid identification up to four generations of backcrossing fall within practical operational limits of most commercial platforms currently available for genotyping low-density SNP marker panels. Therefore, cost-effective assay panels could be developed to provide practical tools for accurate species-purity certification.", "gemini": "Lai tạo giữa các loài đã được sử dụng rộng rãi để tạo ra các loài động vật và thực vật phù hợp hơn cho sản xuất thức ăn và sợi kể từ thời La Mã, và vẫn quan trọng trong các hoạt động nông nghiệp hiện tại với các công dụng ngày càng tăng, đặc biệt là trong nuôi trồng thủy sản. Các công cụ chẩn đoán dựa trên các bảng đánh dấu với số lượng đánh dấu đủ để xác định chính xác các cá thể lai giữa các loài từ quần thể lai ghép và lai ngược ngày càng cần thiết cho chứng nhận độ tinh khiết của loài và quản lý đàn giống thương mại một cách chính xác và thiết thực. Số lượng tối thiểu các dấu hiệu di-alen với các alen đặc hiệu cho loài cần thiết để xác định chính xác các cá thể lai trong quần thể lai ghép và lai ngược nâng cao đã được ước tính bằng cách sử dụng phân tích công suất và dao động từ 5 đến 191 (α = .05) và từ 7 đến 293 (α = .01), lần lượt xem xét các quần thể lai ngược 1 (BC1) đến BC6. Số lượng dấu hiệu cần thiết để xác định lai chính xác được quan sát thấy trong các quần thể BC1 đến BC6 mô phỏng dao động từ 5 đến 1.131 và 7 đến 8.065, lần lượt xem xét tỷ lệ lỗi ≤ 5% và ≤ 1%. Số lượng dấu hiệu chẩn đoán ước tính và quan sát được cần thiết để xác định lai chính xác lên đến bốn thế hệ lai ngược nằm trong giới hạn hoạt động thực tế của hầu hết các nền tảng thương mại hiện có để tạo kiểu gen các bảng đánh dấu SNP mật độ thấp. Do đó, có thể phát triển các bảng xét nghiệm hiệu quả về chi phí để cung cấp các công cụ thiết thực cho chứng nhận độ tinh khiết của loài một cách chính xác."} +{"idx": 1870512, "en": "The spinocerebellar ataxia type 2 is a neurodegenerative disease with autosomal dominant inheritance; clinically characterized by progressive cerebellar ataxia, slow ocular saccades, nystagmus, ophthalmoplegia, dysarthria, dysphagia, cognitive deterioration, mild dementia, peripheral neuropathy. Infantile onset is a rare presentation that only has been reported in four instances in the literature. In the present work a boy aged 5 years 7 months was studied due to horizontal gaze-evoked nystagmus, without saccades, ataxic gait, dysarthria, dysphagia, dysmetria, generalized spasticity mainly pelvic, bilateral Babinsky. The mother aged 27 years-old presented progressive cerebellar ataxia, dysarthria, dysmetria, dysdiadochokinesis, limb ataxia and olivopontocerebellar atrophy. The molecular analysis was made by identifying the expansion repeats in tandem by long PCR to analyze the repeats in the ATXN2 gene. We found an extreme CAG expansion repeats of ~884 repeats in the child. We describe a Mexican child affected by SCA2 with an infantile onset, associated with a high number of CAG repeats previously no reported and anticipation phenomenon.", "gemini": "Rối loạn thoái hóa tiểu não kiểu 2 là một bệnh thoái hóa thần kinh có tính di truyền trội trên nhiễm sắc thể thường; đặc điểm lâm sàng là thoái hóa tiểu não tiến triển, chuyển động mắt chậm, rung giật nhãn cầu, liệt cơ mắt, nói khó, nuốt khó, suy giảm nhận thức, mất trí nhẹ, bệnh thần kinh ngoại biên. Khởi phát ở trẻ sơ sinh là một biểu hiện hiếm gặp chỉ được báo cáo trong bốn trường hợp trong tài liệu. Trong công trình hiện tại, một bé trai 5 tuổi 7 tháng được nghiên cứu do rung giật nhãn cầu theo hướng nhìn ngang, không có chuyển động mắt, dáng đi không vững, nói khó, nuốt khó, mất điều hòa, co cứng toàn thân chủ yếu ở vùng chậu, Babinsky hai bên. Người mẹ 27 tuổi có biểu hiện thoái hóa tiểu não tiến triển, nói khó, mất điều hòa, mất điều hòa vận động, mất điều hòa chi và teo olivopontocerebellar. Phân tích phân tử được thực hiện bằng cách xác định các đoạn lặp mở rộng theo kiểu song song bằng PCR dài để phân tích các đoạn lặp trong gen ATXN2. Chúng tôi tìm thấy một đoạn lặp CAG mở rộng cực độ gồm ~ 884 đoạn lặp ở đứa trẻ. Chúng tôi mô tả một đứa trẻ Mexico mắc SCA2 khởi phát ở trẻ sơ sinh, liên quan đến một số lượng lớn các đoạn lặp CAG trước đây chưa được báo cáo và hiện tượng dự đoán."} +{"idx": 1870513, "en": "Squamous cell carcinoma of the oral cavity and oropharynx is the sixth most common type of cancer in the world. During tumorigenesis, gene promoter hypermethylation is considered an important mechanism of transcription silencing of tumor suppressor genes, such as DAPK, MGMT and RUNX3. These genes participate in signaling pathways related to apoptosis, DNA repair and proliferation whose loss of expression is possibly associated with cancer development and progression. In order to investigate associations between hypermethylation and clinicopathological and prognostic parameters, promoter methylation was evaluated in 72 HPV negative oral and oropharyngeal tumors using methylation-specific PCR. Hypermethylation frequencies found for DAPK, MGMT and RUNX3 were 38.88%, 19.44% and 1.38% respectively. Patients with MGMT hypermethylation had a better 2-year overall survival compared to patients without methylation. Being MGMT a repair gene for alkylating agents, it could be a biomarker of treatment response for patients who are candidates for cisplatin chemotherapy, predicting drug resistance. In view of the considerable levels of hypermethylation in cancer cells and, for MGMT, its prognostic relevance, DAPK and MGMT show potential as epigenetic markers, in a way that additional studies may test its viability and efficacy in clinical management.", "gemini": "Ung thư biểu mô tế bào vảy ở khoang miệng và hầu họng là loại ung thư phổ biến thứ sáu trên thế giới. Trong quá trình hình thành khối u, sự tăng methyl hóa vùng khởi động gen được coi là một cơ chế quan trọng của quá trình phiên mã làm im lặng các gen ức chế khối u, chẳng hạn như DAPK, MGMT và RUNX3. Các gen này tham gia vào các con đường truyền tín hiệu liên quan đến apoptosis, sửa chữa DNA và tăng sinh, sự mất biểu hiện của chúng có thể liên quan đến sự phát triển và tiến triển của ung thư. Để nghiên cứu mối liên quan giữa tăng methyl hóa và các thông số lâm sàng, bệnh lý và tiên lượng, người ta đã đánh giá sự methyl hóa vùng khởi động ở 72 khối u miệng và hầu họng âm tính với HPV bằng PCR đặc hiệu metyl hóa. Tần suất tăng methyl hóa được tìm thấy đối với DAPK, MGMT và RUNX3 lần lượt là 38,88%, 19,44% và 1,38%. Bệnh nhân tăng methyl hóa MGMT có tỷ lệ sống chung sau 2 năm tốt hơn so với bệnh nhân không methyl hóa. Do MGMT là gen sửa chữa cho các tác nhân alkyl hóa, nên nó có thể là dấu ấn sinh học về đáp ứng điều trị đối với những bệnh nhân là ứng cử viên cho hóa trị cisplatin, dự đoán tình trạng kháng thuốc. Xét đến mức độ tăng methyl hóa đáng kể trong các tế bào ung thư và đối với MGMT, tính liên quan đến tiên lượng của nó, DAPK và MGMT cho thấy tiềm năng làm dấu ấn biểu sinh, theo cách mà các nghiên cứu bổ sung có thể kiểm tra khả năng tồn tại và hiệu quả của nó trong quản lý lâm sàng."} +{"idx": 1870514, "en": "Purpose The circadian clock plays an important role in the expression and regulation of various genes and cellular processes in the body. Here, we study diurnal regulation of the growth and shedding of the sensory axons in the mouse cornea. Methods Male and female BALB/cN mice were euthanized 90 minutes before and after the lights are turned on and off; at 5:30 AM, 8:30 AM, 5:30 PM, and 8:30 PM. Nerve terminal growth, shedding and overall axon density were assessed at these four time points using confocal imaging after staining axons in en face whole mount corneas with antibodies against βIII tubulin, GAP43, and L1CAM. In addition, corneal epithelial cell proliferation, thickness, and desquamation were assessed using ki67, LAMP1, Involucrin, and ZO1. Results Nerve terminal shedding took place between 5:30 AM and 8:30 AM and correlated positively with the timing of apical cell desquamation. After shedding the tips of the nerve terminals, axonal growth increased as indicated by increased axonal GAP43 expression. At 5:30 PM and 8:30 PM before and after the lights are turned off, cell proliferation was reduced, and epithelial thickness was maximal. Conclusions Intraepithelial corneal nerve growth and shedding are under diurnal control regulated by the time of day and whether lights are on or off. Axons extend during the day and are shed within 90 minutes after lights are turned on. The data presented in this article shed light on the potential role that circadian clock plays in corneal pain and discomfort.", "gemini": "Mục đích Đồng hồ sinh học đóng vai trò quan trọng trong việc biểu hiện và điều hòa của nhiều gen và quá trình tế bào trong cơ thể. Ở đây, chúng tôi nghiên cứu sự điều hòa theo nhịp ngày đêm của sự phát triển và rụng của các sợi trục cảm giác ở giác mạc chuột. Phương pháp Chuột BALB/cN đực và cái bị gây chết 90 phút trước và sau khi bật và tắt đèn; lúc 5:30 sáng, 8:30 sáng, 5:30 chiều và 8:30 tối. Sự phát triển, rụng và mật độ sợi trục thần kinh tổng thể được đánh giá tại bốn thời điểm này bằng hình ảnh cộng hưởng sau khi nhuộm sợi trục trên giác mạc toàn bộ đối diện bằng kháng thể chống lại βIII tubulin, GAP43 và L1CAM. Ngoài ra, sự tăng sinh, độ dày và bong tróc của tế bào biểu mô giác mạc được đánh giá bằng ki67, LAMP1, Involucrin và ZO1. Kết quả Sự rụng đầu mút thần kinh diễn ra giữa 5:30 sáng và 8:30 sáng và có tương quan dương với thời điểm bong tróc tế bào đỉnh. Sau khi rụng đầu mút của các đầu mút thần kinh, sự phát triển của sợi trục tăng lên như được chỉ ra bởi biểu hiện GAP43 sợi trục tăng lên. Vào lúc 5:30 chiều và 8:30 tối trước và sau khi tắt đèn, sự tăng sinh tế bào giảm và độ dày biểu mô đạt mức tối đa. Kết luận Sự phát triển và rụng của sợi trục thần kinh biểu mô giác mạc nằm dưới sự kiểm soát theo nhịp ngày đêm được điều chỉnh bởi thời điểm trong ngày và đèn bật hay tắt. Các sợi trục mở rộng vào ban ngày và rụng trong vòng 90 phút sau khi bật đèn. Dữ liệu được trình bày trong bài báo này làm sáng tỏ vai trò tiềm năng mà đồng hồ sinh học đóng trong đau và khó chịu ở giác mạc."} +{"idx": 1870515, "en": "Purpose Diabetic retinopathy (DR) is a microvascular complication caused by prolonged hyperglycemia and characterized by leaky retinal vasculature and ischemia-induced angiogenesis. Vitreous humor is a gel-like biofluid in the posterior segment of the eye between the lens and the retina. Disease-related changes are observed in the biochemical constituents of the vitreous, including proteins and macromolecules. Recently, we found that IL-6 trans-signaling plays a significant role in the vascular leakage and retinal pathology associated with DR. Therefore, in this study, comprehensive proteomic profiling of the murine vitreous was performed to identify diabetes-induced alterations and to determine effects of IL-6 trans-signaling inhibition on these changes. Methods Vitreous samples from mice were collected by evisceration, and proteomic analyses were performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results A total of 154 proteins were identified with high confidence in control mice and were considered to be characteristic of healthy murine vitreous fluid. The levels of 72 vitreous proteins were significantly altered in diabetic mice, including several members of heat shock proteins, 14-3-3 proteins, and tubulins. Alterations in 52 out of 72 proteins in diabetic mice were mitigated by IL-6 trans-signaling inhibition. Conclusions Proteomic analysis of murine vitreous fluid performed in this study provides important information about the changes caused by diabetes in the ocular microenvironment. These diabetes-induced alterations in the murine vitreous proteome were mitigated by IL-6 trans-signaling inhibition. These findings further support that IL-6 trans-signaling may be an important therapeutic target for the treatment of DR.", "gemini": "Mục đích Bệnh võng mạc tiểu đường (DR) là một biến chứng vi mạch do tăng đường huyết kéo dài và đặc trưng bởi mạch máu võng mạc bị rò rỉ và tân mạch do thiếu máu cục bộ. Thủy dịch là một chất lỏng sinh học giống như gel ở phần sau của mắt giữa thủy tinh thể và võng mạc. Những thay đổi liên quan đến bệnh được quan sát thấy trong các thành phần sinh hóa của dịch kính, bao gồm protein và đại phân tử. Gần đây, chúng tôi phát hiện ra rằng truyền tín hiệu xuyên của IL-6 đóng vai trò quan trọng trong tình trạng rò rỉ mạch máu và bệnh lý võng mạc liên quan đến DR. Do đó, trong nghiên cứu này, hồ sơ proteomic toàn diện của dịch kính ở chuột đã được thực hiện để xác định những thay đổi do bệnh tiểu đường gây ra và xác định tác động của việc ức chế truyền tín hiệu xuyên IL-6 đối với những thay đổi này. Phương pháp Các mẫu dịch kính từ chuột được thu thập bằng cách moi mắt và phân tích proteomic được thực hiện bằng phương pháp sắc ký lỏng - phổ khối ghép nối (LC-MS/MS). Kết quả Tổng cộng 154 protein đã được xác định với độ tin cậy cao ở chuột đối chứng và được coi là đặc trưng của dịch kính ở chuột khỏe mạnh. Nồng độ của 72 protein dịch kính bị thay đổi đáng kể ở chuột mắc bệnh tiểu đường, bao gồm một số thành viên của protein sốc nhiệt, protein 14-3-3 và tubulin. Những thay đổi ở 52 trong số 72 protein ở chuột mắc bệnh tiểu đường đã được giảm nhẹ bằng cách ức chế truyền tín hiệu xuyên IL-6. Kết luận Phân tích proteomic của dịch kính ở chuột được thực hiện trong nghiên cứu này cung cấp thông tin quan trọng về những thay đổi do bệnh tiểu đường gây ra trong môi trường vi mô của mắt. Những thay đổi do bệnh tiểu đường gây ra trong proteome dịch kính ở chuột đã được giảm nhẹ bằng cách ức chế truyền tín hiệu xuyên IL-6. Những phát hiện này tiếp tục ủng hộ rằng truyền tín hiệu xuyên IL-6 có thể là một mục tiêu điều trị quan trọng để điều trị DR."} +{"idx": 1870516, "en": "The aim of this research was to evaluate the resorption and osseointegration of an autogenous bone ring, which was grafted in a local vertical alveolar defect with simultaneous implant placement. Six Beagle dogs were enrolled in the study; their 4 nonadjacent mandibular premolars were extracted, and the buccal plate was removed to create bone defects in 2 of the 4 sites. Three months after extraction, Straumann implants (Ø 3.3 mm, length of 8 mm) were placed in the bone defect sites with simultaneous autogenous bone ring grafting and in the conventional extraction sites. After a 3-month healing period and a 3-month loading period, the animals were euthanized. The harvested samples were analyzed using micro-computed tomography (CT) scanning and histological analysis. From the micro-CT measurements, the average vertical bone resorption of the bone ring was 0.23 ± 0.03 mm, which was not significantly different from that around the conventional implant, 0.24 ± 0.12 mm (P > .05). The ratio of the bone volume to the total volume of the bone ring group was 91.11 ± 0.02, which was higher than that of the control group, 88.38 ± 2.34 (P < .05). From the hard tissue section, the bone rings developed fine osseointegration with the implants and the base alveolar bone. The results suggest autogenous bone ring grafting with simultaneous implant placement can survive in a local vertical bone defect with little bone resorption and good osseointegration in dogs with strict management. A bone ring graft must be compared with guided bone regeneration, and a larger and longer observation must be confirmed in clinical patients.", "gemini": "Mục đích của nghiên cứu này là đánh giá sự tái hấp thu và tích hợp xương của một vòng xương tự thân, được ghép vào một khiếm khuyết ổ răng thẳng đứng tại chỗ với việc đặt implant đồng thời. Sáu con chó Beagle được tuyển dụng vào nghiên cứu; 4 răng tiền hàm dưới không liền kề của chúng được nhổ, và mảng má được loại bỏ để tạo ra các khiếm khuyết xương ở 2 trong số 4 vị trí. Ba tháng sau khi nhổ răng, các implant Straumann (Ø 3,3 mm, dài 8 mm) được đặt vào các vị trí khiếm khuyết xương với ghép vòng xương tự thân đồng thời và vào các vị trí nhổ răng thông thường. Sau thời gian lành thương 3 tháng và thời gian chịu tải 3 tháng, các con vật được an tử. Các mẫu thu hoạch được phân tích bằng chụp cắt lớp vi tính (CT) và phân tích mô học. Từ các phép đo vi tính, sự tái hấp thu xương thẳng đứng trung bình của vòng xương là 0,23 ± 0,03 mm, không khác biệt đáng kể so với xung quanh implant thông thường, 0,24 ± 0,12 mm (P > 0,05). Tỷ lệ thể tích xương trên tổng thể tích của nhóm vòng xương là 91,11 ± 0,02, cao hơn nhóm đối chứng, 88,38 ± 2,34 (P < 0,05). Từ phần mô cứng, các vòng xương phát triển tích hợp xương tốt với implant và xương ổ răng gốc. Kết quả cho thấy ghép vòng xương tự thân với việc đặt implant đồng thời có thể tồn tại trong khiếm khuyết xương thẳng đứng tại chỗ với ít tái hấp thu xương và tích hợp xương tốt ở những con chó được quản lý chặt chẽ. Cần so sánh ghép vòng xương với tái tạo xương có hướng dẫn và cần xác nhận quan sát lớn hơn và dài hơn ở bệnh nhân lâm sàng."} +{"idx": 1870517, "en": "Warts, hypogammaglobulinemia, infections, and myelokathexis (WHIM) syndrome is a rare primary immunodeficiency caused by gain-of-function mutations in the CXCR4 gene. We report the safety, tolerability, pharmacokinetics, pharmacodynamics, and preliminary efficacy of mavorixafor from a phase 2 open-label dose-escalation and extension study in 8 adult patients with genetically confirmed WHIM syndrome. Mavorixafor is an oral small molecule selective antagonist of the CXCR4 receptor that increases mobilization and trafficking of white blood cells from the bone marrow. Patients received escalating doses of mavorixafor, up to 400 mg once daily. Five patients continued on the extension study for up to 28.6 months. Mavorixafor was well tolerated with no treatment-related serious adverse events. At a median follow-up of 16.5 months, we observed dose-dependent increases in absolute neutrophil count (ANC) and absolute lymphocyte count (ALC). At doses ≥300 mg/d, ANC was maintained at >500 cells per microliter for a median of 12.6 hours, and ALC was maintained at >1000 cells per microliter for up to 16.9 hours. Continued follow-up on the extension study resulted in a yearly infection rate that decreased from 4.63 events (95% confidence interval, 3.3-6.3) in the 12 months prior to the trial to 2.27 events (95% confidence interval, 1.4-3.5) for patients on effective doses. We observed an average 75% reduction in the number of cutaneous warts. This study demonstrates that mavorixafor, 400 mg once daily, mobilizes neutrophil and lymphocytes in adult patients with WHIM syndrome and provides preliminary evidence of clinical benefit for patients on long-term therapy. The trial was registered at www.clinicaltrials.gov as #NCT03005327.", "gemini": "Hội chứng mụn cóc, giảm gamma globulin máu, nhiễm trùng và myelokathexis (WHIM) là một tình trạng suy giảm miễn dịch nguyên phát hiếm gặp do các đột biến tăng chức năng ở gen CXCR4. Chúng tôi báo cáo về tính an toàn, khả năng dung nạp, dược động học, dược lực học và hiệu quả ban đầu của mavorixafor từ một nghiên cứu mở nhãn tăng liều giai đoạn 2 và nghiên cứu mở rộng ở 8 bệnh nhân trưởng thành được xác nhận về mặt di truyền mắc hội chứng WHIM. Mavorixafor là một phân tử nhỏ đường uống đối kháng chọn lọc thụ thể CXCR4 làm tăng sự huy động và di chuyển của các tế bào bạch cầu từ tủy xương. Bệnh nhân được dùng các liều mavorixafor tăng dần, lên đến 400 mg một lần mỗi ngày. Năm bệnh nhân tiếp tục nghiên cứu mở rộng trong tối đa 28,6 tháng. Mavorixafor được dung nạp tốt mà không có tác dụng phụ nghiêm trọng liên quan đến điều trị. Ở thời điểm theo dõi trung bình là 16,5 tháng, chúng tôi quan sát thấy sự gia tăng phụ thuộc vào liều về số lượng bạch cầu trung tính tuyệt đối (ANC) và số lượng bạch cầu lympho tuyệt đối (ALC). Ở liều ≥300 mg/ngày, ANC được duy trì ở mức >500 tế bào trên microlit trong thời gian trung bình là 12,6 giờ và ALC được duy trì ở mức >1000 tế bào trên microlit trong tối đa 16,9 giờ. Việc theo dõi liên tục trên nghiên cứu mở rộng dẫn đến tỷ lệ nhiễm trùng hàng năm giảm từ 4,63 sự kiện (khoảng tin cậy 95%, 3,3-6,3) trong 12 tháng trước khi thử nghiệm xuống còn 2,27 sự kiện (khoảng tin cậy 95%, 1,4-3,5) đối với những bệnh nhân dùng liều hiệu quả. Chúng tôi quan sát thấy trung bình giảm 75% số lượng mụn cóc trên da. Nghiên cứu này chứng minh rằng mavorixafor, 400 mg một lần mỗi ngày, huy động bạch cầu trung tính và bạch cầu lympho ở những bệnh nhân trưởng thành mắc hội chứng WHIM và cung cấp bằng chứng ban đầu về lợi ích lâm sàng cho những bệnh nhân được điều trị dài hạn. Thử nghiệm đã được đăng ký tại www.clinicaltrials.gov với số #NCT03005327."} +{"idx": 1870518, "en": "BACKGROUND Racial discrimination has been associated with biological dysfunction among ethnic minorities. The extent to which regular physical activity (PA) may buffer this association is unknown. PURPOSE To examine the association between past-year racial discrimination and allostatic load (AL) stratified by PA within a sample of Indigenous adults. METHODS Data were collected from Indigenous adults attending university in a city in western Canada between 2015 and 2017. The Experiences of Discrimination Scale was used to assess discrimination and the Godin-Shephard Leisure-Time Physical Activity Questionnaire assessed PA. A composite of seven biomarkers assessing neuroendocrine, cardiovascular, metabolic, and immune system function measured AL. Linear regression models examined associations adjusted for confounders (N = 150). RESULTS In the insufficiently active group, every 1 point increase in racial discrimination (up to a maximum of 9) resulted in approximately one third of a point increase in AL score. In the sufficiently active group, the association between racial discrimination and AL score was not statistically significant. CONCLUSIONS A growing body of research suggests racial discrimination is associated with multisystem biological dysregulation and health risks. Increased action to address racism in society is a priority. As that work unfolds, there is a need to identify effective tools that racialized groups can use to buffer the effects of racism on their health. The present findings suggest that engagement in regular PA may attenuate the pernicious effects of discrimination on biological dysfunction.", "gemini": "BỐI CẢNH Phân biệt đối xử về chủng tộc có liên quan đến rối loạn chức năng sinh học ở các nhóm dân tộc thiểu số. Mức độ mà hoạt động thể chất (PA) thường xuyên có thể đệm cho mối liên hệ này vẫn chưa được biết. MỤC ĐÍCH Để kiểm tra mối liên hệ giữa phân biệt đối xử về chủng tộc trong năm qua và gánh nặng toàn trạng (AL) được phân tầng theo PA trong một mẫu người lớn bản địa. PHƯƠNG PHÁP Dữ liệu được thu thập từ những người lớn bản địa theo học tại một trường đại học ở một thành phố phía tây Canada trong giai đoạn 2015-2017. Thang đánh giá kinh nghiệm phân biệt đối xử được sử dụng để đánh giá sự phân biệt đối xử và Bản câu hỏi về hoạt động thể chất giải trí Godin-Shephard đánh giá PA. Một hợp chất của bảy chỉ số sinh học đánh giá chức năng hệ thần kinh nội tiết, tim mạch, chuyển hóa và miễn dịch đo AL. Các mô hình hồi quy tuyến tính kiểm tra các hiệp hội đã được điều chỉnh cho các yếu tố gây nhiễu (N = 150). KẾT QUẢ Trong nhóm hoạt động không đủ, cứ tăng 1 điểm về phân biệt đối xử về chủng tộc (tối đa 9 điểm) thì điểm AL tăng khoảng một phần ba điểm. Trong nhóm hoạt động đủ, mối liên hệ giữa phân biệt đối xử về chủng tộc và điểm AL không có ý nghĩa thống kê. KẾT LUẬN Một lượng lớn các nghiên cứu cho thấy phân biệt đối xử về chủng tộc có liên quan đến rối loạn điều hòa sinh học đa hệ thống và rủi ro sức khỏe. Tăng cường hành động để giải quyết nạn phân biệt chủng tộc trong xã hội là một ưu tiên. Khi công việc đó diễn ra, cần xác định các công cụ hiệu quả mà các nhóm bị phân biệt chủng tộc có thể sử dụng để đệm tác động của nạn phân biệt chủng tộc đối với sức khỏe của họ. Những phát hiện hiện tại cho thấy việc tham gia vào PA thường xuyên có thể làm giảm các tác động nguy hại của sự phân biệt đối xử đối với rối loạn chức năng sinh học."} +{"idx": 1870519, "en": "BACKGROUND microRNAs serve as important regulators of the pathogenesis of cardiac hypertrophy. Among them, miR-183 is well documented as a novel tumor suppressor in previous studies, whereas it exhibits a downregulated expression in cardiac hypertrophy recently. The present study was aimed to examine the effect of miR-183 on cardiomyocytes hypertrophy. METHODS Angiotensin II (Ang II) was used for establishment of cardiac hypertrophy model in vitro. Neonatal rat ventricular cardiomyocytes (NRVMs) transfected with miR-183 mimic or negative control were further utilized for the phenotype analysis. Moreover, the bioinformatics analysis and luciferase reporter assays were used for exploring the potential target of miR-183 in cardiomyocytes. RESULTS We observed a significant decreased expression of miR-183 in hypertrophic cardiomyocytes. Overexpression of miR-183 significantly attenuated the cardiomyocytes size morphologically and pro-hypertrophic genes expression. Moreover, we demonstrated that TIAM1 was a direct target gene of miR-183 verified by bioinformatics analysis and luciferase reporter assays, which showed a decreased mRNA and protein expression in the cardiomyocytes transfected with miR-183 upon Ang II stimulation. Additionally, the downregulated TIAM1 expression was required for the attenuated effect of miR-183 on cardiomyocytes hypertrophy. CONCLUSION Taken together, these evidences indicated that miR-183 acted as a cardio-protective regulator for the development of cardiomyocytes hypertrophy via directly regulation of TIAM1.", "gemini": "BỐI CẢNH: microRNA đóng vai trò là chất điều hòa quan trọng trong quá trình sinh bệnh của phì đại cơ tim. Trong số đó, miR-183 được ghi nhận là một chất ức chế khối u mới trong các nghiên cứu trước đây, trong khi gần đây nó lại biểu hiện sự giảm điều hòa trong phì đại cơ tim. Nghiên cứu hiện tại nhằm mục đích kiểm tra tác dụng của miR-183 đối với phì đại tế bào cơ tim. PHƯƠNG PHÁP: Angiotensin II (Ang II) được sử dụng để thiết lập mô hình phì đại cơ tim trong ống nghiệm. Tế bào cơ tim thất của chuột sơ sinh (NRVM) được chuyển gen với miR-183 bắt chước hoặc đối chứng âm tính được sử dụng để phân tích kiểu hình. Hơn nữa, phân tích tin sinh học và xét nghiệm báo cáo luciferase được sử dụng để khám phá mục tiêu tiềm năng của miR-183 trong tế bào cơ tim. KẾT QUẢ: Chúng tôi quan sát thấy sự biểu hiện giảm đáng kể của miR-183 ở tế bào cơ tim phì đại. Sự biểu hiện quá mức của miR-183 làm giảm đáng kể kích thước tế bào cơ tim về mặt hình thái và biểu hiện gen tiền phì đại. Hơn nữa, chúng tôi chứng minh rằng TIAM1 là một gen đích trực tiếp của miR-183 được xác minh bằng phân tích tin sinh học và xét nghiệm báo cáo luciferase, cho thấy sự giảm biểu hiện mRNA và protein ở tế bào cơ tim được chuyển gen với miR-183 sau khi kích thích Ang II. Ngoài ra, biểu hiện TIAM1 giảm điều hòa là cần thiết cho tác dụng giảm nhẹ của miR-183 đối với phì đại tế bào cơ tim. KẾT LUẬN: Tổng hợp lại, những bằng chứng này chỉ ra rằng miR-183 đóng vai trò là chất điều hòa bảo vệ tim đối với sự phát triển của phì đại tế bào cơ tim thông qua điều hòa trực tiếp TIAM1."} +{"idx": 1870520, "en": "Perihematomal edema (PHE) occurs within hours after intracerebral hemorrhage (ICH), leading to secondary injury manifested by impaired blood-brain barrier (BBB) integrity and destruction of adjacent tissue. To dissect the mechanisms underlying PHE formation, we profiled human and mouse perihematomal tissues and identified natural killer (NK) cells as the predominant immune cell subset that outnumbers other infiltrating immune cell types during early stages of ICH. Unbiased clustering of single-cell transcriptional profiles revealed two major NK cell subsets that respectively possess high cytotoxicity or robust chemokine production features in the brain after ICH, distinguishing them from NK cells of the periphery. NK cells exacerbate BBB disruption and brain edema after ICH via cytotoxicity toward cerebral endothelial cells and recruitment of neutrophils that augment focal inflammation. Thus, brain-bound NK cells acquire new features that contribute to PHE formation and neurological deterioration following ICH.", "gemini": "Phù quanh ổ máu (PHE) xảy ra trong vòng vài giờ sau xuất huyết não (ICH), dẫn đến tổn thương thứ phát biểu hiện bằng sự suy giảm tính toàn vẹn của hàng rào máu não (BBB) và phá hủy mô liền kề. Để phân tích các cơ chế cơ bản hình thành PHE, chúng tôi đã lập hồ sơ mô quanh ổ máu ở người và chuột và xác định các tế bào sát thủ tự nhiên (NK) là nhóm tế bào miễn dịch chiếm ưu thế, nhiều hơn các loại tế bào miễn dịch xâm nhập khác trong giai đoạn đầu của ICH. Phân cụ không thiên vị các cấu hình phiên mã của tế bào đơn cho thấy hai nhóm tế bào NK chính tương ứng có các đặc điểm gây độc tế bào cao hoặc sản xuất chemokine mạnh mẽ trong não sau ICH, phân biệt chúng với các tế bào NK ở ngoại vi. Các tế bào NK làm trầm trọng thêm sự phá vỡ BBB và phù não sau ICH thông qua độc tính tế bào đối với các tế bào nội mô não và tuyển dụng các tế bào trung tính làm tăng tình trạng viêm cục bộ. Do đó, các tế bào NK liên kết với não có được các đặc điểm mới góp phần vào sự hình thành PHE và suy thoái thần kinh sau ICH."} +{"idx": 1870521, "en": "The APOBEC3 family of antiviral DNA cytosine deaminases is implicated as the second largest source of mutation in cancer. This mutational process may be a causal driver or inconsequential passenger to the overall tumor phenotype. We show that human APOBEC3A expression in murine colon and liver tissues increases tumorigenesis. All other APOBEC3 family members, including APOBEC3B, fail to promote liver tumor formation. Tumor DNA sequences from APOBEC3A-expressing animals display hallmark APOBEC signature mutations in TCA/T motifs. Bioinformatic comparisons of the observed APOBEC3A mutation signature in murine tumors, previously reported APOBEC3A and APOBEC3B mutation signatures in yeast, and reanalyzed APOBEC mutation signatures in human tumor datasets support cause-and-effect relationships for APOBEC3A-catalyzed deamination and mutagenesis in driving multiple human cancers.", "gemini": "Họ APOBEC3 của các chất khử amin cytosine DNA chống vi-rút được coi là nguồn đột biến lớn thứ hai trong ung thư. Quá trình đột biến này có thể là động lực gây ra hoặc là hành khách không quan trọng đối với kiểu hình khối u nói chung. Chúng tôi chỉ ra rằng biểu hiện APOBEC3A của người ở mô ruột kết và gan của chuột làm tăng quá trình sinh khối u. Tất cả các thành viên khác trong họ APOBEC3, bao gồm cả APOBEC3B, đều không thúc đẩy sự hình thành khối u ở gan. Trình tự DNA của khối u từ các động vật biểu hiện APOBEC3A hiển thị các đột biến đặc trưng của APOBEC trong các chủ đề TCA/T. Các so sánh tin sinh học về đặc điểm đột biến APOBEC3A quan sát được ở các khối u ở chuột, các đặc điểm đột biến APOBEC3A và APOBEC3B đã được báo cáo trước đây ở nấm men và các đặc điểm đột biến APOBEC được phân tích lại trong các tập dữ liệu khối u của người hỗ trợ mối quan hệ nhân quả đối với quá trình khử amin và gây đột biến do APOBEC3A xúc tác trong việc thúc đẩy nhiều loại ung thư ở người."} +{"idx": 1870522, "en": "Preexisting/pregestational diabetes enhances the risk of birth defects. Several factors have been involved during the implantation process, such as cytokines (granulocyte-macrophage-colony-stimulating factor [GM-CSF]). The objective was to evaluate the effects of two levels of diabetes on the redox status of preimplantation embryos during the implantation process to comprehend how both are involved in embryo and fetal viability against maternal diabetes. Female Sprague-Dawley rats received streptozotocin at birth (mild diabetes [MD]) or at adulthood (severe diabetes [SD]) to obtain two experimental diabetes intensities. After confirming the diabetic status, the nondiabetic and diabetic groups were mated around day 110 of life. At gestational day (GD) 21, fetuses were assessed for viability and malformations and ovaries for embryo loss before implantation. Other pregnant nondiabetic and diabetic rats were sacrificed at GD2-4 for maternal and preimplantation embryo oxidative stress markers, maternal serum insulin, uterine fluid GM-CSF, and preimplantation embryo morphological analysis. MD and SD caused abnormal redox levels, lower GM-CSF and insulin levels during the preimplantation period, and embryonic loss before implantation. SD caused lower fetal viability and higher fetal malformation percentages at GD21. The SD dam-derived preimplantation embryos presented lower glutathione levels and higher thiobarbituric acid reactive substances concentration at GD3 and an increased frequency of abnormal preimplantation embryos at GD4. In conclusion, preexisting diabetes leads to complications in the implantation process. Furthermore, maternal oxidative stress and other metabolic changes alter the redox state and morphological structure of preimplantation embryos, contributing to damaged growth and development in late pregnancy.", "gemini": "Tiểu đường tiền thai kỳ làm tăng nguy cơ dị tật bẩm sinh. Một số yếu tố đã tham gia vào quá trình làm tổ, chẳng hạn như cytokine (yếu tố kích thích tạo thành tập đoàn hạt-đại thực bào [GM-CSF]). Mục tiêu là đánh giá tác động của hai mức độ tiểu đường đối với trạng thái oxy hóa khử của phôi trước khi cấy trong quá trình làm tổ để hiểu cách cả hai tham gia vào khả năng sống của phôi và thai nhi chống lại bệnh tiểu đường của mẹ. Chuột Sprague-Dawley cái được tiêm streptozotocin khi sinh (tiểu đường nhẹ [MD]) hoặc khi trưởng thành (tiểu đường nặng [SD]) để có được hai cường độ tiểu đường thực nghiệm. Sau khi xác nhận tình trạng tiểu đường, nhóm không tiểu đường và nhóm tiểu đường được giao phối vào khoảng ngày 110 của cuộc đời. Vào ngày mang thai (GD) 21, các thai nhi được đánh giá về khả năng sống và dị tật và buồng trứng để mất phôi trước khi làm tổ. Những con chuột không tiểu đường và tiểu đường mang thai khác đã bị hy sinh ở GD2-4 để tìm các dấu hiệu căng thẳng oxy hóa của phôi trước khi cấy và của mẹ, insulin huyết thanh của mẹ, GM-CSF trong dịch tử cung và phân tích hình thái phôi trước khi cấy. MD và SD gây ra mức độ oxy hóa khử bất thường, mức GM-CSF và insulin thấp hơn trong thời kỳ trước khi cấy và mất phôi trước khi cấy. SD gây ra tỷ lệ sống của thai nhi thấp hơn và tỷ lệ dị tật thai nhi cao hơn ở GD21. Phôi trước khi cấy có nguồn gốc từ đập SD biểu hiện mức độ glutathione thấp hơn và nồng độ chất phản ứng với axit thiobarbituric cao hơn ở GD3 và tần suất phôi trước khi cấy bất thường tăng lên ở GD4. Tóm lại, bệnh tiểu đường tiền thai kỳ dẫn đến các biến chứng trong quá trình làm tổ. Hơn nữa, stress oxy hóa của mẹ và các thay đổi chuyển hóa khác làm thay đổi trạng thái oxy hóa khử và cấu trúc hình thái của phôi trước khi cấy, góp phần làm hỏng sự phát triển và phát triển vào cuối thai kỳ."} +{"idx": 1870523, "en": "Rapid growth of single-cell transcriptomic data provides unprecedented opportunities for close scrutinizing of dynamical cellular processes. Through investigating epithelial-to-mesenchymal transition (EMT), we develop an integrative tool that combines unsupervised learning of single-cell transcriptomic data and multiscale mathematical modeling to analyze transitions during cell fate decision. Our approach allows identification of individual cells making transition between all cell states, and inference of genes that drive transitions. Multiscale extractions of single-cell scale outputs naturally reveal intermediate cell states (ICS) and ICS-regulated transition trajectories, producing emergent population-scale models to be explored for design principles. Testing on the newly designed single-cell gene regulatory network model and applying to twelve published single-cell EMT datasets in cancer and embryogenesis, we uncover the roles of ICS on adaptation, noise attenuation, and transition efficiency in EMT, and reveal their trade-off relations. Overall, our unsupervised learning method is applicable to general single-cell transcriptomic datasets, and our integrative approach at single-cell resolution may be adopted for other cell fate transition systems beyond EMT.", "gemini": "Sự phát triển nhanh chóng của dữ liệu phiên mã đơn tế bào mang lại những cơ hội chưa từng có để xem xét kỹ lưỡng các quá trình tế bào động lực. Thông qua việc điều tra quá trình chuyển đổi biểu mô-trung mô (EMT), chúng tôi phát triển một công cụ tích hợp kết hợp việc học không có giám sát về dữ liệu phiên mã đơn tế bào và mô hình toán học đa tỷ lệ để phân tích các quá trình chuyển đổi trong quá trình quyết định số phận tế bào. Phương pháp tiếp cận của chúng tôi cho phép xác định các tế bào riêng lẻ tạo nên quá trình chuyển đổi giữa tất cả các trạng thái tế bào và suy luận các gen thúc đẩy quá trình chuyển đổi. Việc trích xuất đa tỷ lệ đầu ra ở quy mô tế bào đơn một cách tự nhiên sẽ tiết lộ các trạng thái tế bào trung gian (ICS) và các quỹ đạo chuyển đổi do ICS điều chỉnh, tạo ra các mô hình quy mô dân số mới nổi để khám phá các nguyên tắc thiết kế. Kiểm tra trên mô hình mạng điều hòa gen đơn tế bào được thiết kế mới và áp dụng cho mười hai bộ dữ liệu EMT đơn tế bào đã công bố về ung thư và phôi thai, chúng tôi khám phá vai trò của ICS trong quá trình thích ứng, giảm tiếng ồn và hiệu quả chuyển đổi trong EMT và tiết lộ mối quan hệ đánh đổi của chúng. Nhìn chung, phương pháp học không có giám sát của chúng tôi có thể áp dụng cho các bộ dữ liệu phiên mã đơn tế bào nói chung và phương pháp tiếp cận tích hợp của chúng tôi ở độ phân giải đơn tế bào có thể được áp dụng cho các hệ thống chuyển đổi số phận tế bào khác ngoài EMT."} +{"idx": 1870524, "en": "Excess platelet activation by extracellular vesicles (EVs) results in trophoblast inflammasome activation, interleukin 1β (IL-1β) activation, preeclampsia (PE), and partial embryonic lethality. Embryonic thrombomodulin (TM) deficiency, which causes embryonic lethality hallmarked by impaired trophoblast proliferation, has been linked with maternal platelet activation. We hypothesized that placental TM loss, platelet activation, and embryonic lethality are mechanistically linked to trophoblast inflammasome activation. Here, we uncover unidirectional interaction of placental inflammasome activation and reduced placental TM expression: although inflammasome inhibition did not rescue TM-null embryos from lethality, the inflammasome-dependent cytokine IL-1β reduced trophoblast TM expression and impaired pregnancy outcome. EVs, known to induce placental inflammasome activation, reduced trophoblast TM expression and proliferation. Trophoblast TM expression correlated negatively with IL-1β expression and positively with platelet numbers and trophoblast proliferation in human PE placentae, implying translational relevance. Soluble TM treatment or placental TM restoration ameliorated the EV-induced PE-like phenotype in mice, preventing placental thromboinflammation and embryonic death. The lethality of TM-null embryos is not a consequence of placental NLRP3 inflammasome activation. Conversely, EV-induced placental inflammasome activation reduces placental TM expression, promoting placental and embryonic demise. These data identify a new function of placental TM in PE and suggest that soluble TM limits thromboinflammatory pregnancy complications.", "gemini": "Hoạt hóa tiểu cầu quá mức do các túi ngoại bào (EV) dẫn đến hoạt hóa thể viêm bào thai, hoạt hóa interleukin 1β (IL-1β), tiền sản giật (PE) và tử vong một phần của phôi. Thiếu thrombomodulin (TM) ở phôi, gây ra tử vong ở phôi có đặc điểm là tăng sinh tế bào nuôi dưỡng bị suy yếu, có liên quan đến hoạt hóa tiểu cầu của mẹ. Chúng tôi đưa ra giả thuyết rằng mất TM ở nhau thai, hoạt hóa tiểu cầu và tử vong ở phôi có liên quan về mặt cơ chế đến hoạt hóa thể viêm bào thai. Ở đây, chúng tôi khám phá ra sự tương tác đơn hướng của hoạt hóa thể viêm bào thai và giảm biểu hiện TM ở nhau thai: mặc dù việc ức chế thể viêm không cứu được phôi không có TM khỏi tử vong, nhưng cytokine IL-1β phụ thuộc vào thể viêm làm giảm biểu hiện TM ở tế bào nuôi dưỡng và làm suy yếu kết quả của thai kỳ. Các EV, được biết là gây ra hoạt hóa thể viêm bào thai, làm giảm biểu hiện và tăng sinh TM ở tế bào nuôi dưỡng. Biểu hiện TM ở tế bào nuôi dưỡng tương quan âm với biểu hiện IL-1β và tương quan dương với số lượng tiểu cầu và tăng sinh tế bào nuôi dưỡng ở nhau thai PE của người, ngụ ý có liên quan đến dịch thuật. Điều trị bằng TM hòa tan hoặc phục hồi TM ở nhau thai làm giảm kiểu hình giống PE do EV gây ra ở chuột, ngăn ngừa tình trạng viêm tắc mạch ở nhau thai và tử vong ở phôi. Tính gây tử vong của phôi không có TM không phải là hậu quả của việc hoạt hóa thể viêm NLRP3 ở nhau thai. Ngược lại, hoạt hóa thể viêm bào thai do EV gây ra làm giảm biểu hiện TM ở nhau thai, thúc đẩy tử vong ở nhau thai và phôi. Những dữ liệu này xác định một chức năng mới của TM ở nhau thai trong PE và cho thấy rằng TM hòa tan hạn chế các biến chứng thai kỳ do viêm tắc mạch."} +{"idx": 1870525, "en": "CONTEXT Functional hypothalamic amenorrhea (HA) is a common, acquired form of hypogonadotropic hypogonadism that occurs in the setting of energy deficits and/or stress. Variability in individual susceptibility to these stressors, HA heritability, and previous identification of several rare sequence variants (RSVs) in genes associated with the rare disorder, isolated hypogonadotropic hypogonadism (IHH), in individuals with HA suggest a possible genetic contribution to HA susceptibility. OBJECTIVE We sought to determine whether the burden of RSVs in IHH-related genes is greater in women with HA than controls. DESIGN We compared patients with HA to control women. SETTING The study was conducted at secondary referral centers. PATIENTS AND OTHER PARTICIPANTS Women with HA (n = 106) and control women (ClinSeq study; n = 468). INTERVENTIONS We performed exome sequencing in all patients and controls. MAIN OUTCOME MEASURE(S) The frequency of RSVs in 53 IHH-associated genes was determined using rare variant burden and association tests. RESULTS RSVs were overrepresented in women with HA compared with controls (P = .007). Seventy-eight heterozygous RSVs in 33 genes were identified in 58 women with HA (36.8% of alleles) compared to 255 RSVs in 41 genes among 200 control women (27.2%). CONCLUSIONS Women with HA are enriched for RSVs in genes that cause IHH, suggesting that variation in genes associated with gonadotropin-releasing hormone neuronal ontogeny and function may be a major determinant of individual susceptibility to developing HA in the face of diet, exercise, and/or stress.", "gemini": "Bối cảnh Thiểu năng buồng trứng dưới đồi chức năng (HA) là một dạng phổ biến, mắc phải của tình trạng giảm tiết gonadotropin gây giảm tiết gonad khiến buồng trứng không hoạt động, xảy ra trong bối cảnh thiếu năng lượng và/hoặc căng thẳng. Sự thay đổi về mức độ nhạy cảm của từng cá nhân đối với những tác nhân gây căng thẳng này, khả năng di truyền HA và việc trước đây đã xác định được một số biến thể trình tự hiếm (RSV) trong các gen liên quan đến rối loạn hiếm gặp, tình trạng giảm tiết gonadotropin gây giảm tiết gonad khiến buồng trứng không hoạt động (IHH) ở những cá nhân bị HA gợi ý rằng có thể có yếu tố di truyền góp phần vào khả năng mắc HA. MỤC TIÊU Chúng tôi tìm cách xác định xem gánh nặng RSV trong các gen liên quan đến IHH có lớn hơn ở những phụ nữ bị HA so với nhóm đối chứng hay không. THIẾT KẾ Chúng tôi đã so sánh những bệnh nhân bị HA với phụ nữ đối chứng. BỐI CẢNH Nghiên cứu được tiến hành tại các trung tâm giới thiệu thứ cấp. BỆNH NHÂN VÀ NHỮNG NGƯỜI THAM GIA KHÁC Phụ nữ bị HA (n = 106) và phụ nữ đối chứng (nghiên cứu ClinSeq; n = 468). CAN THIỆP Chúng tôi đã tiến hành giải trình tự toàn bộ exome ở tất cả bệnh nhân và nhóm đối chứng. CÁC BIỆN PHÁP ĐO KẾT QUẢ CHÍNH Tần suất RSV trong 53 gen liên quan đến IHH đã được xác định bằng cách sử dụng gánh nặng biến thể hiếm và các xét nghiệm liên kết. KẾT QUẢ RSV xuất hiện nhiều hơn ở những phụ nữ bị HA so với nhóm đối chứng (P = .007). Đã xác định được bảy mươi tám RSV dị hợp tử trong 33 gen ở 58 phụ nữ bị HA (36,8% alen) so với 255 RSV trong 41 gen ở 200 phụ nữ đối chứng (27,2%). KẾT LUẬN Những phụ nữ bị HA có nhiều RSV trong các gen gây ra IHH, gợi ý rằng sự biến đổi trong các gen liên quan đến sự phát sinh và chức năng của hormon giải phóng gonadotropin có thể là yếu tố quyết định chính đến khả năng mắc HA của từng cá nhân khi đối mặt với chế độ ăn uống, tập thể dục và/hoặc căng thẳng."} +{"idx": 1870526, "en": "Nivolumab, an anti-programmed death-1 (PD-1) monoclonal antibody, showed promising activity in relapsed or refractory (R/R) follicular lymphoma (FL) in a phase 1 study. We conducted a phase 2 trial to further evaluate its efficacy and safety in patients with R/R FL and to explore biomarkers of response. Patients with R/R FL and at least 2 prior lines of therapy, each containing a CD20 antibody or an alkylating agent, were treated with nivolumab 3 mg/kg every 2 weeks. The primary end point was objective response rate (ORR) assessed by an independent radiologic review committee. Biomarker analyses included gene expression profiling and multiplex immunofluorescence studies of pretreatment tumor samples. A total of 92 patients were treated. After a minimum follow-up of 12 months, ORR was 4% (4 of 92 patients). Median progression-free survival (PFS) was 2.2 months (95% confidence interval [CI], 1.9-3.6 months). Median duration of response was 11 months (95% CI, 8-14 months). Exploratory analyses suggested that responders had significantly higher proportion of CD3+ T cells in the tumor microenvironment than nonresponders, but no significant differences in PD-1 or programmed death-ligand 1 expression were observed. High expression of a set of tumor-associated macrophage genes was associated with reduced PFS (hazard ratio, 3.28; 95% CI, 1.76-6.11; P = .001). The safety profile was consistent with previous reports of nivolumab. In conclusion, nivolumab monotherapy was associated with very limited activity in patients with R/R FL. Better understanding of the immune biology of this disease may facilitate the development of effective checkpoint-based strategies. This trial was registered at www.clinicaltrials.gov as #NCT02038946.", "gemini": "Nivolumab, một kháng thể đơn dòng kháng chết theo chương trình-1 (PD-1), đã cho thấy hoạt động đầy hứa hẹn ở bệnh u lympho nang (FL) tái phát hoặc kháng trị (R/R) trong một nghiên cứu giai đoạn 1. Chúng tôi đã tiến hành một thử nghiệm giai đoạn 2 để đánh giá thêm hiệu quả và tính an toàn của thuốc ở những bệnh nhân bị FL R/R và để khám phá các dấu ấn sinh học về đáp ứng. Những bệnh nhân bị FL R/R và ít nhất 2 phác đồ điều trị trước đó, mỗi phác đồ có chứa kháng thể CD20 hoặc tác nhân alkyl hóa, đã được điều trị bằng nivolumab 3 mg/kg cách 2 tuần. Điểm cuối chính là tỷ lệ đáp ứng khách quan (ORR) được đánh giá bởi một ủy ban đánh giá hình ảnh độc lập. Các phân tích dấu ấn sinh học bao gồm lập hồ sơ biểu hiện gen và các nghiên cứu miễn dịch huỳnh quang đa kênh về các mẫu khối u tiền xử lý. Tổng cộng có 92 bệnh nhân được điều trị. Sau thời gian theo dõi tối thiểu là 12 tháng, ORR là 4% (4/92 bệnh nhân). Thời gian sống không tiến triển (PFS) trung bình là 2,2 tháng (khoảng tin cậy 95% [CI], 1,9-3,6 tháng). Thời gian đáp ứng trung bình là 11 tháng (95% CI, 8-14 tháng). Các phân tích thăm dò cho thấy những người đáp ứng có tỷ lệ tế bào T CD3+ cao hơn đáng kể trong môi trường vi mô của khối u so với những người không đáp ứng, nhưng không thấy có sự khác biệt đáng kể nào về biểu hiện PD-1 hoặc phối tử chết theo chương trình-1. Biểu hiện cao của một tập hợp các gen đại thực bào liên quan đến khối u có liên quan đến PFS giảm (tỷ lệ nguy cơ, 3,28; 95% CI, 1,76-6,11; P = .001). Hồ sơ an toàn phù hợp với các báo cáo trước đây về nivolumab. Tóm lại, liệu pháp đơn trị bằng nivolumab có liên quan đến hoạt động rất hạn chế ở những bệnh nhân bị FL R/R. Hiểu rõ hơn về sinh học miễn dịch của căn bệnh này có thể tạo điều kiện thuận lợi cho việc phát triển các chiến lược dựa trên điểm kiểm soát hiệu quả. Thử nghiệm này đã được đăng ký tại www.clinicaltrials.gov với số #NCT02038946."} +{"idx": 1870527, "en": "hnRNPA2 is a major component of mRNA transport granules in oligodendrocytes and neurons. However, the structural details of how hnRNPA2 binds the A2 recognition element (A2RE) and if this sequence stimulates granule formation by enhancing phase separation of hnRNPA2 has not yet been studied. Using solution NMR and biophysical studies, we find that each of the two individual RRMs retain the domain structure observed in complex with RNA but are not rigidly confined (i.e. they move independently) in solution in the absence of RNA. hnRNPA2 RRMs bind the minimal rA2RE11 weakly but at least, and most likely, two hnRNPA2 molecules are able to simultaneously bind the longer 21mer myelin basic protein A2RE. Upon binding of the RNA, NMR chemical shift deviations are observed in both RRMs, suggesting both play a role in binding the A2RE11. Interestingly, addition of short A2RE RNAs or longer RNAs containing this sequence completely prevents in vitro phase separation of full-length hnRNPA2 and aggregation of the disease-associated mutants. These findings suggest that RRM interactions with specific recognition sequences alone do not account for nucleating granule formation, consistent with models where multivalent protein:RNA and protein:protein contacts form across many sites in granule proteins and long RNA transcripts.", "gemini": "hnRNPA2 là một thành phần chính của các hạt vận chuyển mRNA trong các tế bào thần kinh đệm và tế bào thần kinh. Tuy nhiên, các chi tiết về cấu trúc về cách hnRNPA2 liên kết với yếu tố nhận dạng A2 (A2RE) và liệu trình tự này có kích thích sự hình thành hạt bằng cách tăng cường sự phân tách pha của hnRNPA2 hay không vẫn chưa được nghiên cứu. Sử dụng các nghiên cứu NMR về dung dịch và sinh lý, chúng tôi thấy rằng mỗi RRM riêng lẻ đều giữ lại cấu trúc miền được quan sát thấy trong phức hợp với RNA nhưng không bị giới hạn cứng nhắc (tức là chúng di chuyển độc lập) trong dung dịch khi không có RNA. Các RRM của hnRNPA2 liên kết yếu với rA2RE11 tối thiểu nhưng ít nhất và rất có thể, hai phân tử hnRNPA2 có thể liên kết đồng thời với protein cơ bản myelin dài hơn 21mer A2RE. Khi liên kết với RNA, độ lệch dịch hóa học NMR được quan sát thấy ở cả hai RRM, cho thấy cả hai đều đóng vai trò trong việc liên kết với A2RE11. Điều thú vị là việc bổ sung các RNA A2RE ngắn hoặc các RNA dài hơn có chứa trình tự này hoàn toàn ngăn chặn sự phân tách pha trong ống nghiệm của hnRNPA2 toàn phần và sự kết tập của các đột biến liên quan đến bệnh. Những phát hiện này cho thấy rằng các tương tác RRM với các trình tự nhận dạng cụ thể không chỉ là nguyên nhân hình thành hạt, phù hợp với các mô hình mà ở đó các tiếp xúc đa hóa trị protein:RNA và protein:protein hình thành trên nhiều vị trí trong các protein hạt và bản phiên mã RNA dài."} +{"idx": 1870528, "en": "RecJ reportedly participates in the base excision repair (BER) pathway, but structural and functional data are scarce. Herein, the Deinococcus radiodurans RecJ (drRecJ) deletion strain exhibited extreme sensitivity to hydrogen peroxide and methyl-methanesulphonate, as well as a high spontaneous mutation rate and an accumulation of unrepaired abasic sites in vivo, indicating the involvement of drRecJ in the BER pathway. The binding affinity and nuclease activity preference of drRecJ toward DNA substrates containing a 5'-P-dSpacer group, a 5'-deoxyribose-phosphate (dRP) mimic, were established. A 1.9 Å structure of drRecJ in complex with 5'-P-dSpacer-modified single-stranded DNA (ssDNA) revealed a 5'-monophosphate binding pocket and occupancy of 5'-dRP in the drRecJ nuclease core. The mechanism for RecJ 5'-dRP catalysis was explored using structural and biochemical data, and the results implied that drRecJ is not a canonical 5'-dRP lyase. Furthermore, in vitro reconstitution assays indicated that drRecJ tends to participate in the long-patch BER pathway rather than the short-patch BER pathway.", "gemini": "RecJ được báo cáo là tham gia vào con đường sửa chữa cắt bỏ bazơ (BER), nhưng dữ liệu về cấu trúc và chức năng còn rất ít. Ở đây, chủng đột biến xóa Deinococcus radiodurans RecJ (drRecJ) biểu hiện độ nhạy cực cao với hydro peroxide và methyl-methanesulfonate, cũng như tỷ lệ đột biến tự phát cao và sự tích tụ các vị trí không có bazơ chưa được sửa chữa trong cơ thể sống, cho thấy sự tham gia của drRecJ trong con đường BER. Độ ái lực liên kết và sở thích hoạt động của nuclease của drRecJ đối với các chất nền DNA có chứa nhóm 5'-P-dSpacer, một chất bắt chước 5'-deoxyribose-phosphate (dRP), đã được thiết lập. Cấu trúc 1,9 Å của drRecJ trong phức hợp với DNA mạch đơn (ssDNA) được sửa đổi bằng 5'-P-dSpacer cho thấy túi liên kết 5'-monophosphate và sự chiếm chỗ của 5'-dRP trong lõi nuclease drRecJ. Cơ chế xúc tác 5'-dRP của RecJ đã được khám phá bằng cách sử dụng dữ liệu cấu trúc và sinh hóa, và kết quả ngụ ý rằng drRecJ không phải là lyase 5'-dRP điển hình. Hơn nữa, các xét nghiệm tái tạo trong ống nghiệm chỉ ra rằng drRecJ có xu hướng tham gia vào con đường BER vá dài chứ không phải con đường BER vá ngắn."} +{"idx": 1870529, "en": "The purpose of this in vitro study was to evaluate the influence of taper angle in internal conical connections of implant systems and the application of chlorhexidine gel as an antibacterial agent or polyvinyl siloxane sealant on reverse torque values of abutment screws after dynamic loading. Four implant systems having different taper angles (5.4°, 12°, 45°, 60°) were tested in this study. Test specimens were divided into 3 groups: control (neither chlorhexidine gel filled nor silicone sealed), 2% chlorhexidine gel filled, and silicone sealed. The samples were subjected to a dynamic load of 50 N at 1 Hz for 500 000 cycles before reverse torque measurements. The taper angle of conical connections presented a quantitative positive correlation between the degree of the taper angle and the percentage of tightening torque loss. However, it was significant only between 60° angled connection and others except for the sealant applied groups (P = .013 for control groups and P = .007 for chlorhexidine groups). The percentages of decrease in torque values of silicon sealant-applied specimens were significantly higher than both the control and chlorhexidine groups (P values are .001, .002, .001, and .002, respectively, according to increasing taper angles), but the percentage of decrease in torque values due to chlorhexidine application was not statistically significant when compared with control groups. Gel form chlorhexidine application as an antibacterial agent does not significantly affect the implant-abutment connection stability under dynamic loads. Polyvinyl siloxane sealant may cause screw loosening under functional loads.", "gemini": "Mục đích của nghiên cứu trong ống nghiệm này là đánh giá ảnh hưởng của góc côn trong các kết nối hình nón bên trong của hệ thống cấy ghép và ứng dụng gel chlorhexidine như một tác nhân kháng khuẩn hoặc chất trám polyvinyl siloxane lên các giá trị mô men xoắn ngược của vít trụ sau khi tải động. Bốn hệ thống cấy ghép có góc côn khác nhau (5,4°, 12°, 45°, 60°) đã được thử nghiệm trong nghiên cứu này. Các mẫu thử được chia thành 3 nhóm: nhóm đối chứng (không chứa đầy gel chlorhexidine hoặc niêm phong silicon), nhóm chứa đầy gel chlorhexidine 2% và nhóm niêm phong silicon. Các mẫu được chịu tải động 50 N ở tần số 1 Hz trong 500.000 chu kỳ trước khi đo mô men xoắn ngược. Góc côn của các kết nối hình nón thể hiện mối tương quan dương định lượng giữa độ lớn của góc côn và tỷ lệ phần trăm mất mô men xoắn siết chặt. Tuy nhiên, nó chỉ có ý nghĩa giữa kết nối góc 60° và các kết nối khác ngoại trừ các nhóm áp dụng chất trám (P = 0,013 cho nhóm đối chứng và P = 0,007 cho nhóm chlorhexidine). Tỷ lệ phần trăm giảm giá trị mô men xoắn của các mẫu áp dụng chất trám silicon cao hơn đáng kể so với cả nhóm đối chứng và nhóm chlorhexidine (giá trị P lần lượt là 0,001, 0,002, 0,001 và 0,002 theo góc côn tăng dần), nhưng tỷ lệ phần trăm giảm giá trị mô men xoắn do ứng dụng chlorhexidine không có ý nghĩa thống kê khi so sánh với nhóm đối chứng. Dạng gel chlorhexidine ứng dụng như một tác nhân kháng khuẩn không ảnh hưởng đáng kể đến độ ổn định của kết nối cấy ghép-trụ dưới tải trọng động. Chất trám polyvinyl siloxane có thể làm ốc vít bị lỏng dưới tải trọng chức năng."} +{"idx": 1870530, "en": "Antisense oligonucleotide (ASO) drugs that trigger RNase H1 cleavage of target RNAs have been developed to treat various diseases. Basic pharmacological principles suggest that the development of tolerance is a common response to pharmacological interventions. In this manuscript, for the first time we report a molecular mechanism of tolerance that occurs with some ASOs. Two observations stimulated our interest: some RNA targets are difficult to reduce with RNase H1 activating ASOs and some ASOs display a shorter duration of activity than the prolonged target reduction typically observed. We found that certain ASOs targeting the coding region of some mRNAs that initially reduce target mRNAs can surprisingly increase the levels of the corresponding pre-mRNAs. The increase in pre-mRNA is delayed and due to enhanced transcription and likely also slower processing. This process requires that the ASOs bind in the coding region and reduce the target mRNA by RNase H1 while the mRNA resides in the ribosomes. The pre-mRNA increase is dependent on UPF3A and independent of the NMD pathway or the XRN1-CNOT pathway. The response is consistent in multiple cell lines and independent of the methods used to introduce ASOs into cells.", "gemini": "Các loại thuốc oligonucleotide phản nghĩa (ASO) kích hoạt sự phân cắt RNase H1 của các RNA đích đã được phát triển để điều trị nhiều bệnh khác nhau. Các nguyên tắc dược lý cơ bản cho thấy rằng sự phát triển của tình trạng dung nạp là một phản ứng phổ biến đối với các can thiệp dược lý. Trong bản thảo này, lần đầu tiên chúng tôi báo cáo một cơ chế phân tử về tình trạng dung nạp xảy ra với một số ASO. Hai quan sát đã kích thích sự quan tâm của chúng tôi: một số đích RNA khó giảm bằng ASO kích hoạt RNase H1 và một số ASO thể hiện thời gian hoạt động ngắn hơn so với việc giảm đích kéo dài thường thấy. Chúng tôi phát hiện ra rằng một số ASO nhắm vào vùng mã hóa của một số mRNA ban đầu có thể làm giảm mRNA đích lại có thể làm tăng đáng ngạc nhiên nồng độ của các tiền mRNA tương ứng. Sự gia tăng tiền mRNA bị trì hoãn và do phiên mã tăng cường và có thể cũng chậm xử lý hơn. Quá trình này đòi hỏi ASO phải liên kết trong vùng mã hóa và giảm mRNA đích bằng RNase H1 trong khi mRNA nằm trong ribosome. Sự gia tăng tiền mRNA phụ thuộc vào UPF3A và không phụ thuộc vào con đường NMD hoặc con đường XRN1-CNOT. Phản ứng này nhất quán ở nhiều dòng tế bào và không phụ thuộc vào các phương pháp được sử dụng để đưa ASO vào tế bào."} +{"idx": 1870531, "en": "INTRODUCTION The purpose of this study was to evaluate optimal neutral wrist splint duration in treatment-naive mild and mild to moderate carpal tunnel syndrome (CTS). Neutral wrist splinting is a conservative treatment for CTS; however, no clear guidelines exist for how long splinting should be prescribed for optimal outcomes. MATERIALS AND METHODS In this prospective clinical trial subjects were assigned to wear a neutral wrist splint for 6 (group A) or 12 weeks (group B). Symptom and functional outcomes were assessed by questionnaire at baseline, 6 and 12 weeks. Median nerve sensory and motor latencies were recorded at baseline and 12 weeks. Pretreatment, midtreatment, and posttreatment measures were compared within and between treatment groups where applicable. This study was approved by the United States Air Force Academy institutional review board. RESULTS A total of 30 subjects (37 hands) were randomized to 2 treatment groups. Mean symptom severity and median sensory peak latency significantly improved in both groups at 12 weeks, without a significant difference between groups. Mean functional severity significantly improved only in group A. CONCLUSION Neutral wrist splint for 6 weeks resulted in better clinical improvements in patients with untreated mild and mild to moderate CTS. There was no additional benefit in extending splinting 6 additional weeks. This is the first study to compare duration of wrist splint use beyond 8 weeks. A larger sample size is needed to identify the reason for lack of functional improvement in group B. Long-term follow-up of this cohort will be helpful to determine the natural history of initial wrist splint use.", "gemini": "MỤC ĐÍCH Nghiên cứu này nhằm đánh giá thời gian đeo nẹp cổ tay trung tính tối ưu trong điều trị hội chứng ống cổ tay (CTS) nhẹ và mức độ nhẹ đến trung bình ở những bệnh nhân chưa từng điều trị. Đeo nẹp cổ tay trung tính là phương pháp điều trị bảo tồn đối với CTS; tuy nhiên, chưa có hướng dẫn rõ ràng về thời gian đeo nẹp để có kết quả tối ưu. VẬT LIỆU VÀ PHƯƠNG PHÁP Trong thử nghiệm lâm sàng triển vọng này, các đối tượng được chỉ định đeo nẹp cổ tay trung tính trong 6 (nhóm A) hoặc 12 tuần (nhóm B). Các triệu chứng và kết quả chức năng được đánh giá bằng bảng câu hỏi vào thời điểm ban đầu, 6 và 12 tuần. Độ trễ cảm giác và vận động của dây thần kinh giữa được ghi lại vào thời điểm ban đầu và 12 tuần. Các phép đo trước điều trị, giữa điều trị và sau điều trị được so sánh trong và giữa các nhóm điều trị khi có thể áp dụng. Nghiên cứu này đã được hội đồng đánh giá thể chế của Học viện Không quân Hoa Kỳ chấp thuận. KẾT QUẢ Tổng cộng có 30 đối tượng (37 bàn tay) được phân ngẫu nhiên vào 2 nhóm điều trị. Mức độ nghiêm trọng của triệu chứng trung bình và độ trễ đỉnh cảm giác trung bình được cải thiện đáng kể ở cả hai nhóm sau 12 tuần, không có sự khác biệt đáng kể giữa các nhóm. Mức độ nghiêm trọng về chức năng trung bình chỉ cải thiện đáng kể ở nhóm A. KẾT LUẬN Đeo nẹp cổ tay trung tính trong 6 tuần dẫn đến cải thiện lâm sàng tốt hơn ở những bệnh nhân bị CTS nhẹ và mức độ nhẹ đến trung bình chưa được điều trị. Không có lợi ích bổ sung nào khi kéo dài thời gian đeo nẹp thêm 6 tuần. Đây là nghiên cứu đầu tiên so sánh thời gian đeo nẹp cổ tay kéo dài hơn 8 tuần. Cần có kích thước mẫu lớn hơn để xác định lý do thiếu cải thiện chức năng ở nhóm B. Việc theo dõi lâu dài nhóm đối tượng này sẽ hữu ích để xác định diễn biến tự nhiên của việc đeo nẹp cổ tay ban đầu."} +{"idx": 1870532, "en": "Bud maturation is a physiological process that implies a set of morphophysiological changes that lead to the transition of growth patterns from young to mature. This transition defines tree growth and architecture, and in consequence traits such as biomass production and wood quality. In Pinus pinaster Aiton, a conifer of great timber value, bud maturation is closely related to polycyclism (multiple growth periods per year). This process causes a lack of apical dominance, and consequently increased branching that reduces its timber quality and value. However, despite its importance, little is known about bud maturation. In this work, proteomics and metabolomics were employed to study apical and basal sections of young and mature buds in P. pinaster. Proteins and metabolites in samples were described and quantified using (n)UPLC-LTQ-Orbitrap. The datasets were analyzed employing an integrative statistical approach, which allowed the determination of the interactions between proteins and metabolites and the different bud sections and ages. Specific dynamics of proteins and metabolites such as histones H3 and H4, ribosomal proteins L15 and L12, chaperonin TCP1, 14-3-3 protein gamma, gibberellins A1, A3 and A8, strigolactones and abscisic acid, involved in epigenetic regulation, proteome remodeling, hormonal signaling and abiotic stress pathways showed their potential role during bud maturation. Candidates and pathways were validated employing interaction databases and targeted transcriptomics. These results increase our understanding of the molecular processes behind bud maturation, a key step towards improving timber production and natural pine forests management in a future scenario of climate change. However, further studies are necessary using different P. pinaster populations that show contrasting wood quality and stress tolerance in order to generalize the results.", "gemini": "Sự trưởng thành của chồi là một quá trình sinh lý học ngụ ý một loạt các thay đổi hình thái sinh lý dẫn đến sự chuyển đổi các mô hình tăng trưởng từ non sang trưởng thành. Sự chuyển đổi này xác định sự tăng trưởng và kiến trúc của cây, và do đó các đặc điểm như sản xuất sinh khối và chất lượng gỗ. Ở Pinus pinaster Aiton, một loài cây lá kim có giá trị gỗ lớn, sự trưởng thành của chồi có liên quan chặt chẽ đến đa chu kỳ (nhiều chu kỳ tăng trưởng trong một năm). Quá trình này gây ra sự thiếu ưu thế đỉnh, và do đó làm tăng sự phân nhánh làm giảm chất lượng và giá trị gỗ của cây. Tuy nhiên, mặc dù có tầm quan trọng như vậy, người ta vẫn biết rất ít về sự trưởng thành của chồi. Trong công trình này, proteomics và metabolomics đã được sử dụng để nghiên cứu các phần đỉnh và gốc của các chồi non và trưởng thành ở P. pinaster. Các protein và chất chuyển hóa trong mẫu đã được mô tả và định lượng bằng (n) UPLC-LTQ-Orbitrap. Các tập dữ liệu được phân tích bằng cách sử dụng một phương pháp tiếp cận thống kê tích hợp, cho phép xác định các tương tác giữa protein và chất chuyển hóa và các phần và độ tuổi khác nhau của chồi. Động lực cụ thể của các protein và chất chuyển hóa như histon H3 và H4, protein ribosome L15 và L12, chaperonin TCP1, protein gamma 14-3-3, gibberellin A1, A3 và A8, strigolacton và axit abscisic, liên quan đến điều hòa biểu sinh, tái tạo proteome, tín hiệu nội tiết tố và con đường căng thẳng phi sinh học cho thấy vai trò tiềm năng của chúng trong quá trình trưởng thành của chồi. Các ứng cử viên và con đường đã được xác nhận bằng cách sử dụng cơ sở dữ liệu tương tác và phiên mã mục tiêu. Những kết quả này làm tăng sự hiểu biết của chúng ta về các quá trình phân tử đằng sau sự trưởng thành của chồi, một bước quan trọng hướng tới việc cải thiện sản xuất gỗ và quản lý rừng thông tự nhiên trong bối cảnh biến đổi khí hậu trong tương lai. Tuy nhiên, cần có thêm các nghiên cứu sử dụng các quần thể P. pinaster khác nhau có chất lượng gỗ và khả năng chịu căng thẳng tương phản để khái quát hóa các kết quả."} +{"idx": 1870533, "en": "The purpose of this study was to analyze the risk of the maxillary sinus lift technique and the correlation between the thickness of the gingiva, maxillary sinus membrane, and the maxillary sinus lateral wall. Cone-beam computerized tomograhy (CBCT) records of 32 adult dentate patients (10 male/22 female) were analyzed. The gingival thickness records of the dental units were compared with the thickness measurements of the membrane and lateral wall of the maxillary sinus. The gingival biotypes varied between 1.1 mm (thin) and 1.6 mm (thick), with a small association with sex. The thickness of the sinus membrane presented a small association between sexes (0.2 mm, female/0.3 mm, male) and gingival biotypes (Cohen d = .52). The lateral wall presented a weak association between the biotypes (1.3 mm, thin/1.1 mm, thick). There was also no correlation between the membrane and lateral wall (r = -.22). The volume dimension related to the graft area of the sinus was 4 mm3 for men and 5 mm3 for women. There was a weak correlation of gingival thickness compared with membrane thickness and lateral wall of the sinus (r = .304/r = -.31). Gingival thickness does not appear to be a reliable thickness predictor of the membrane or lateral wall of the maxillary sinus. The analysis of maxillary sinus anatomical structures through CBCT is the most reliable technique to identify the thickness of the membrane and lateral wall of the maxillary sinus before surgery. We believe that new studies are necessary to confirm our findings.", "gemini": "Mục đích của nghiên cứu này là phân tích nguy cơ của kỹ thuật nâng xoang hàm trên và mối tương quan giữa độ dày của nướu, màng xoang hàm trên và thành bên xoang hàm trên. Hồ sơ chụp cắt lớp vi tính chùm tia hình nón (CBCT) của 32 bệnh nhân răng trưởng thành (10 nam / 22 nữ) đã được phân tích. Hồ sơ độ dày nướu của các đơn vị răng đã được so sánh với các phép đo độ dày của màng và thành bên của xoang hàm trên. Các kiểu hình nướu thay đổi từ 1,1 mm (mỏng) đến 1,6 mm (dày), có liên quan nhỏ đến giới tính. Độ dày của màng xoang có liên quan nhỏ giữa các giới tính (0,2 mm, nữ / 0,3 mm, nam) và các kiểu hình nướu (Cohen d = 0,52). Thành bên có liên quan yếu giữa các kiểu hình (1,3 mm, mỏng / 1,1 mm, dày). Cũng không có mối tương quan giữa màng và thành bên (r = -0,22). Kích thước thể tích liên quan đến vùng ghép của xoang là 4 mm3 đối với nam và 5 mm3 đối với nữ. Có một mối tương quan yếu giữa độ dày nướu so với độ dày màng và thành bên của xoang (r = 0,304 / r = -0,31). Độ dày nướu dường như không phải là yếu tố dự đoán độ dày đáng tin cậy của màng hoặc thành bên của xoang hàm trên. Việc phân tích các cấu trúc giải phẫu của xoang hàm trên thông qua CBCT là kỹ thuật đáng tin cậy nhất để xác định độ dày của màng và thành bên của xoang hàm trên trước khi phẫu thuật. Chúng tôi tin rằng cần có những nghiên cứu mới để xác nhận những phát hiện của chúng tôi."} +{"idx": 1870534, "en": "Plants need efficient nitrate (NO3-) sensing systems and sophisticated signaling pathways to develop a wide range of adaptive responses to external fluctuations of NO3- supply. In Arabidopsis thaliana, numerous molecular regulators have been identified to participate in signaling pathways that respond specifically to NO3-. In contrast, only a single NO3- sensing system has been described to date, relying on the NRT1.1 (NPF6.3/CHL1) NO3- transceptor. NRT1.1 governs a wide range of responses to NO3-, from fast reprogramming of genome expression (the primary nitrate response) to longer-term developmental changes (effects on lateral root development). NRT1.1 appears to be at the center of a complex network of signaling pathways, involving numerous molecular players acting downstream and/or upstream of it. Interestingly, some of these regulators are involved in crosstalk with the signaling pathways of other nutrients, such as inorganic phosphate or potassium. Although NRT1.1-mediated NO3- sensing and signaling has mostly been documented in Arabidopsis, recent evidence indicates that similar mechanisms involving NRT1.1 orthologues are operative in rice. This review aims to delineate how the NRT1.1 sensing system and the downstream/upstream transduction cascades are integrated to control both the expression of NO3--responsive genes and the induced plasticity of root development.", "gemini": "Cây cần có hệ thống cảm biến nitrat (NO3-) hiệu quả và các con đường truyền tín hiệu phức tạp để phát triển nhiều phản ứng thích ứng với sự biến động bên ngoài của nguồn cung NO3-. Ở Arabidopsis thaliana, nhiều chất điều hòa phân tử đã được xác định tham gia vào các con đường truyền tín hiệu phản ứng cụ thể với NO3-. Ngược lại, cho đến nay chỉ có một hệ thống cảm biến NO3- được mô tả, dựa trên thụ thể NO3- NRT1.1 (NPF6.3/CHL1). NRT1.1 điều chỉnh nhiều phản ứng với NO3-, từ việc lập trình lại nhanh chóng biểu hiện của bộ gen (phản ứng nitrat chính) đến những thay đổi phát triển dài hạn (ảnh hưởng đến sự phát triển của rễ bên). NRT1.1 dường như là trung tâm của một mạng lưới phức tạp các con đường truyền tín hiệu, liên quan đến nhiều tác nhân phân tử hoạt động hạ nguồn và/hoặc thượng nguồn của nó. Điều thú vị là một số chất điều hòa này có liên quan đến tương tác với các con đường truyền tín hiệu của các chất dinh dưỡng khác, chẳng hạn như photphat vô cơ hoặc kali. Mặc dù cảm biến và truyền tín hiệu NO3- do NRT1.1 trung gian chủ yếu được ghi nhận ở Arabidopsis, nhưng các bằng chứng gần đây chỉ ra rằng các cơ chế tương tự liên quan đến các ortholog NRT1.1 cũng hoạt động ở lúa. Mục đích của bài tổng quan này là mô tả cách hệ thống cảm biến NRT1.1 và các tầng truyền dẫn hạ nguồn/thượng nguồn được tích hợp để kiểm soát cả biểu hiện của các gen phản ứng với NO3- và tính dẻo được cảm ứng của sự phát triển rễ."} +{"idx": 1870535, "en": "RNA G-quadruplexes (G4s) are secondary structures proposed to function as regulators of post-transcriptional mRNA localisation and translation. G4s within some neuronal mRNAs are known to control distal localisation and local translation, contributing to distinct local proteomes that facilitate the synaptic remodelling attributed to normal cellular function. In this study, we characterise the G4 formation of a (GGN)13 repeat found within the 5' UTR of the potassium 2-pore domain leak channel Task3 mRNA. Biophysical analyses show that this (GGN)13 repeat forms a parallel G4 in vitro exhibiting the stereotypical potassium specificity of G4s, remaining thermostable under physiological ionic conditions. Through mouse brain tissue G4-RNA immunoprecipitation, we further confirm that Task3 mRNA forms a G4 structure in vivo. The G4 is inhibitory to translation of Task3 in vitro and is overcome through activity of a G4-specific helicase DHX36, increasing K+ leak currents and membrane hyperpolarisation in HEK293 cells. Further, we observe that this G4 is fundamental to ensuring delivery of Task3 mRNA to distal primary cortical neurites. It has been shown that aberrant Task3 expression correlates with neuronal dysfunction, we therefore posit that this G4 is important in regulated local expression of Task3 leak channels that maintain K+ leak within neurons.", "gemini": "G-quadruplexes (G4s) của RNA là các cấu trúc thứ cấp được đề xuất có chức năng như các chất điều hòa dịch mã và định vị mRNA sau phiên mã. G4s trong một số mRNA của tế bào thần kinh được biết là kiểm soát định vị xa và dịch mã cục bộ, góp phần tạo nên các proteom cục bộ riêng biệt tạo điều kiện cho việc tái tạo synap được quy cho chức năng tế bào bình thường. Trong nghiên cứu này, chúng tôi mô tả đặc điểm hình thành G4 của đoạn lặp (GGN)13 nằm trong vùng UTR 5' của mRNA kênh rò rỉ miền 2 lỗ kali Task3. Các phân tích sinh lý cho thấy đoạn lặp (GGN)13 này tạo thành G4 song song trong ống nghiệm, biểu hiện tính đặc hiệu với kali theo khuôn mẫu của G4, vẫn ổn định nhiệt ở các điều kiện ion sinh lý. Thông qua kết tủa miễn dịch G4-RNA của mô não chuột, chúng tôi xác nhận thêm rằng mRNA Task3 tạo thành cấu trúc G4 trong cơ thể sống. G4 ức chế quá trình dịch mã của Task3 trong ống nghiệm và bị khắc phục thông qua hoạt động của helicase DHX36 đặc hiệu với G4, làm tăng dòng rò K+ và tăng phân cực màng ở các tế bào HEK293. Hơn nữa, chúng tôi quan sát thấy rằng G4 này rất cần thiết để đảm bảo truyền tải mRNA Task3 đến các sợi trục thần kinh vỏ não chính xa. Người ta đã chỉ ra rằng biểu hiện Task3 bất thường có tương quan với rối loạn chức năng thần kinh, do đó, chúng tôi đưa ra giả thuyết rằng G4 này rất quan trọng trong việc điều hòa biểu hiện cục bộ của các kênh rò rỉ Task3 duy trì rò rỉ K+ trong các tế bào thần kinh."} +{"idx": 1870536, "en": "Importance Severe obesity and its related diseases, such as type 2 diabetes, hypertension, dyslipidemia, and sleep apnea, are very common in the United States, but currently very few patients with these conditions choose to undergo bariatric surgery. Summaries of the expanding evidence for both the benefits and risks of bariatric surgery are needed to better guide shared decision-making conversations. Observations There are approximately 252 000 bariatric procedures (per 2018 numbers) performed each year in the US, of which an estimated 15% are revisions. The 1991 National Institutes of Health guidelines recommended consideration of bariatric surgery in patients with a body mass index (calculated as weight in kilograms divided by height in meters squared) of 40 or higher or 35 or higher with serious obesity-related comorbidities. These guidelines are still widely used; however, there is increasing evidence that bariatric procedures should also be considered for patients with type 2 diabetes and a body mass index of 30 to 35 if hyperglycemia is inadequately controlled despite optimal medical treatment for type 2 diabetes. Substantial evidence indicates that surgery results in greater improvements in weight loss and type 2 diabetes outcomes, compared with nonsurgical interventions, regardless of the type of procedures used. The 2 most common procedures used currently, the sleeve gastrectomy and gastric bypass, have similar effects on weight loss and diabetes outcomes and similar safety through at least 5-year follow-up. However, emerging evidence suggests that the sleeve procedure is associated with fewer reoperations, and the bypass procedure may lead to more durable weight loss and glycemic control. Although safety is a concern, current data indicate that the perioperative mortality rates range from 0.03% to 0.2%, which has substantially improved since early 2000s. More long-term randomized studies are needed to assess the effect of bariatric procedures on cardiovascular disease, cancer, and other health outcomes and to evaluate emerging newer procedures. Conclusions and Relevance Modern bariatric procedures have strong evidence of efficacy and safety. All patients with severe obesity-and especially those with type 2 diabetes-should be engaged in a shared decision-making conversation about the risks and benefits of surgery compared with continuing usual medical and lifestyle treatment, and the decision about surgery should be driven primarily by informed patient preferences.", "gemini": "Tầm quan trọng Béo phì nghiêm trọng và các bệnh liên quan như đái tháo đường týp 2, tăng huyết áp, rối loạn lipid máu và ngưng thở khi ngủ rất phổ biến ở Hoa Kỳ, nhưng hiện nay rất ít bệnh nhân mắc các tình trạng này lựa chọn phẫu thuật cắt bỏ dạ dày. Tóm tắt về bằng chứng ngày càng tăng cho cả lợi ích và rủi ro của phẫu thuật cắt bỏ dạ dày là cần thiết để hướng dẫn tốt hơn các cuộc trò chuyện ra quyết định chung. Quan sát Có khoảng 252.000 ca phẫu thuật cắt bỏ dạ dày (theo số liệu năm 2018) được thực hiện mỗi năm ở Hoa Kỳ, trong đó ước tính 15% là phẫu thuật sửa đổi. Các hướng dẫn của Viện Y tế Quốc gia năm 1991 khuyến nghị xem xét phẫu thuật cắt bỏ dạ dày ở những bệnh nhân có chỉ số khối cơ thể (tính bằng cân nặng tính bằng kilogam chia cho chiều cao tính bằng mét bình phương) từ 40 trở lên hoặc từ 35 trở lên kèm theo các bệnh đi kèm nghiêm trọng liên quan đến béo phì. Các hướng dẫn này vẫn được sử dụng rộng rãi; tuy nhiên, ngày càng có nhiều bằng chứng cho thấy các thủ thuật cắt bỏ dạ dày cũng nên được xem xét cho những bệnh nhân mắc bệnh tiểu đường týp 2 và chỉ số khối cơ thể từ 30 đến 35 nếu tình trạng tăng đường huyết không được kiểm soát đầy đủ mặc dù đã điều trị nội khoa tối ưu cho bệnh tiểu đường týp 2. Các bằng chứng đáng kể chỉ ra rằng phẫu thuật mang lại hiệu quả cải thiện cân nặng và kết quả bệnh tiểu đường týp 2 tốt hơn so với các biện pháp can thiệp không phẫu thuật, bất kể loại thủ thuật nào được sử dụng. 2 thủ thuật phổ biến nhất hiện nay là cắt dạ dày tay áo và bắc cầu dạ dày, có tác dụng tương tự nhau đối với kết quả giảm cân và bệnh tiểu đường cũng như độ an toàn tương tự trong thời gian theo dõi ít nhất 5 năm. Tuy nhiên, các bằng chứng mới cho thấy rằng thủ thuật cắt tay áo ít phải phẫu thuật lại hơn, còn thủ thuật bắc cầu có thể giúp giảm cân và kiểm soát đường huyết lâu dài hơn. Mặc dù tính an toàn là một mối quan tâm, nhưng dữ liệu hiện tại chỉ ra rằng tỷ lệ tử vong trong thời kỳ quanh phẫu thuật dao động từ 0,03% đến 0,2%, đã cải thiện đáng kể kể từ đầu những năm 2000. Cần có nhiều nghiên cứu ngẫu nhiên dài hạn hơn để đánh giá tác dụng của các thủ thuật cắt bỏ dạ dày đối với bệnh tim mạch, ung thư và các kết cục sức khỏe khác cũng như để đánh giá các thủ thuật mới nổi. Kết luận và Tầm quan trọng Các thủ thuật cắt bỏ dạ dày hiện đại có bằng chứng mạnh mẽ về hiệu quả và tính an toàn. Tất cả những bệnh nhân bị béo phì nghiêm trọng - và đặc biệt là những bệnh nhân mắc bệnh tiểu đường týp 2 - nên tham gia vào cuộc trò chuyện ra quyết định chung về rủi ro và lợi ích của phẫu thuật so với việc tiếp tục điều trị y tế và lối sống thông thường, và quyết định về phẫu thuật chủ yếu nên được thúc đẩy bởi sở thích đã được thông báo của bệnh nhân."} +{"idx": 1870537, "en": "In August 2017, the USS Bataan received a mass casualty incident (MCI) of 6 foreign special forces operators after a helicopter crash. All 6 patients were medically evacuated successfully to the USS Bataan, and all patients survived and were successfully returned to their allied country. Four of the patients received whole blood with 2 receiving over 10 units of blood or massive transfusions. One patient required 44 units of blood, and at 1 point in his resuscitation, he received 12 units of whole blood every 30 minutes. Due to administrative factors outside of the ship's control, these 6 patients had prolonged stabilization during the MCI. This factor differentiates this MCI on the USS Bataan from previous cases. Internal medicine trained physicians with their expertise in inpatient care, postsurgical management, and critical care were instrumental in sustaining these casualties in this prolonged stabilization environment. In the era of distributed maritime operations, where casualty-receiving ships will experience more geographic and resource isolation, there is a potential for the need for prolonged stabilization above the 6 to 12-hour window typical of role II platforms. The known increase in cardiac and pulmonary morbidity and mortality with medical evacuation delay highlights the importance of internal medicine physicians in the role II setting. It is critical that we emphasize the inpatient and critical care principles of these patients in the prolonged field care environment.", "gemini": "Vào tháng 8 năm 2017, tàu USS Bataan đã tiếp nhận một vụ tai nạn thương vong hàng loạt (MCI) gồm 6 đặc nhiệm nước ngoài sau vụ rơi trực thăng. Cả 6 bệnh nhân đều được sơ tán y tế thành công đến tàu USS Bataan, và tất cả bệnh nhân đều sống sót và được đưa trở về nước đồng minh của họ thành công. Bốn trong số các bệnh nhân đã được truyền máu toàn phần, trong đó 2 bệnh nhân được truyền hơn 10 đơn vị máu hoặc truyền máu ồ ạt. Một bệnh nhân cần truyền 44 đơn vị máu và tại một thời điểm hồi sức, bệnh nhân này đã được truyền 12 đơn vị máu toàn phần sau mỗi 30 phút. Do các yếu tố hành chính ngoài tầm kiểm soát của tàu, 6 bệnh nhân này đã kéo dài thời gian ổn định trong quá trình MCI. Yếu tố này phân biệt MCI này trên tàu USS Bataan với các trường hợp trước đây. Các bác sĩ được đào tạo về nội khoa với chuyên môn về chăm sóc nội trú, quản lý hậu phẫu và chăm sóc đặc biệt đã đóng vai trò quan trọng trong việc duy trì những thương vong này trong môi trường ổn định kéo dài này. Trong kỷ nguyên của các hoạt động hàng hải phân tán, nơi các tàu tiếp nhận thương vong sẽ trải qua nhiều sự cô lập về địa lý và nguồn lực hơn, có khả năng cần kéo dài thời gian ổn định trên khung thời gian từ 6 đến 12 giờ thông thường của nền tảng vai trò II. Tỷ lệ mắc bệnh và tử vong về tim mạch và phổi tăng đã biết khi trì hoãn sơ tán y tế làm nổi bật tầm quan trọng của các bác sĩ nội khoa trong bối cảnh vai trò II. Điều quan trọng là chúng ta phải nhấn mạnh các nguyên tắc chăm sóc nội trú và chăm sóc đặc biệt của những bệnh nhân này trong môi trường chăm sóc thực địa kéo dài."} +{"idx": 1870538, "en": "This study aimed to compare the crestal bone loss between a 2-implant-supported, noncantilevered 3-unit fixed partial prosthesis (TUFPP) with sinus augmentation and a 2-implant-supported, distal cantilevered TUFPP without sinus augmentation in the posterior maxilla having insufficient crestal bone. The study subjects were enrolled in 2 groups. Group 1 included patients with 2 implants: an anterior implant placed in the native bone and a posterior implant inserted with simultaneous sinus augmentation using a xenogenic bone graft to support a TUFPP. Group 2 included patients with 2 implants inserted in the native bone to support a distal cantilevered TUFPP. The crestal bone levels at the distal and mesial aspects of each implant were measured at baseline and 6-, 12-, and 24-month follow-up controls on panoramic radiographs. Fifty-two patients and 104 implants were included. There was a significant difference in distal crestal bone loss between anterior and posterior implants in group 1 at 6 months (P < .05) but not at 12 and 24 months (P > .05). Distal crestal bone loss was significantly increased in group 1 posterior implants compared to the group 2 posterior implants at 6 months (P < .05). There was no significant difference in mesial bone loss between the anterior and posterior implants in both groups at all follow-up controls (P > .05). There was also no significant mesial crestal bone loss in relation to the anterior and posterior implants of both groups at all follow-up controls (P > .05). Noncantilevered 2-implant-supported TUFPP with sinus augmentation may have similar medium-term crestal bone loss when compared to cantilevered 2-implant-supported TUFPP without sinus augmentation. Further prospective studies should be designed to compare the performance of the 2-implant-supported cantilevered TUFPP and 2-implant-supported TUFPP with sinus augmentation.", "gemini": "Nghiên cứu này nhằm so sánh tình trạng mất xương mào giữa phục hình cố định 3 đơn vị không nhô ra được hỗ trợ bằng 2 implant (TUFPP) với nâng xoang và phục hình cố định 3 đơn vị nhô ra xa được hỗ trợ bằng 2 implant (TUFPP) không nâng xoang ở xương hàm trên sau có xương mào không đủ. Các đối tượng nghiên cứu được chia thành 2 nhóm. Nhóm 1 bao gồm những bệnh nhân có 2 implant: một implant trước được đặt trong xương gốc và một implant sau được lắp đồng thời với việc nâng xoang bằng ghép xương dị loại để hỗ trợ TUFPP. Nhóm 2 bao gồm những bệnh nhân có 2 implant được lắp vào xương gốc để hỗ trợ TUFPP nhô ra xa. Mức xương mào ở mặt xa và mặt gần của mỗi implant được đo ở mức cơ bản và theo dõi 6, 12 và 24 tháng trên phim X-quang toàn cảnh. Có 52 bệnh nhân và 104 implant được đưa vào nghiên cứu. Có sự khác biệt đáng kể về tình trạng mất xương mào xa giữa các implant trước và sau ở nhóm 1 sau 6 tháng (P < 0,05) nhưng không có sự khác biệt sau 12 và 24 tháng (P > 0,05). Tình trạng mất xương mào xa tăng đáng kể ở các implant sau của nhóm 1 so với các implant sau của nhóm 2 sau 6 tháng (P < 0,05). Không có sự khác biệt đáng kể về tình trạng mất xương giữa các implant trước và sau ở cả hai nhóm tại tất cả các lần theo dõi (P > 0,05). Cũng không có tình trạng mất xương mào giữa đáng kể liên quan đến các implant trước và sau của cả hai nhóm tại tất cả các lần theo dõi (P > 0,05). TUFPP không nhô ra được hỗ trợ bằng 2 implant với nâng xoang có thể có tình trạng mất xương mào trung hạn tương tự khi so sánh với TUFPP nhô ra được hỗ trợ bằng 2 implant không nâng xoang. Nên thiết kế thêm các nghiên cứu triển vọng để so sánh hiệu quả của TUFPP nhô ra được hỗ trợ bằng 2 implant và TUFPP không nhô ra được hỗ trợ bằng 2 implant với nâng xoang."} +{"idx": 1870539, "en": "This study compared peri-implant vertical bone loss, the periodontal index, prosthodontic maintenance requirements and oral health-related quality of life (OHQoL) of patients using mandibular implant-supported overdentures with 2 different attachment systems after an average of 5 years of use. Sixteen mandibular overdenture patients with magnet attachments (group 3), 24 with implant-supported mandibular overdentures with Locator attachments (group 2), and 25 conventional complete denture patients (group 1) were included in the study. The existing at least 5 years old dentures of all patients were evaluated for prosthodontic maintenance by the same prosthodontist and the patients were administered the oral health impact profile-14-Turkish version (OHIP-TR-14) questionnaire. Peri-implant tissue health was evaluated clinically in terms of plaque scores, bleeding scores, probing depth, and gingival index. Radiographic evaluation was performed in terms of peri-implant vertical bone loss. Radiographic evaluations were performed 5 years after overdenture insertion. There were no statistically significant differences between the groups in terms of total OHIP-TR-14 scores (P > .05). Although the average vertical bone resorption measured in group 2 at the end of 5 years was 1.38 mm, there was an average vertical bone loss of 1.45 mm in group 3, but this difference was not statistically significant (P > .05). Among the periodontal health indicators, there were no statistically significant differences in the mean values for periodontal indexes. According to results of the study, there were no differences in peri-implant vertical bone loss in groups 2 and 3, in periodontal health in mandibular overdentures with groups 2 and 3, or between total OHIP-TR-14 scores of patients in all groups.", "gemini": "Nghiên cứu này so sánh tình trạng mất xương thẳng đứng quanh implant, chỉ số nha chu, nhu cầu bảo trì phục hình và chất lượng cuộc sống liên quan đến sức khỏe răng miệng (OHQoL) của những bệnh nhân sử dụng hàm giả trên implant hỗ trợ ở hàm dưới với 2 hệ thống gắn khác nhau sau trung bình 5 năm sử dụng. Mười sáu bệnh nhân có hàm giả trên hàm dưới với nam châm gắn (nhóm 3), 24 bệnh nhân có hàm giả trên hàm dưới hỗ trợ implant với chốt định vị (nhóm 2) và 25 bệnh nhân có hàm giả hoàn chỉnh thông thường (nhóm 1) được đưa vào nghiên cứu. Hàm giả hiện có ít nhất 5 năm tuổi của tất cả bệnh nhân được một bác sĩ phục hình đánh giá về việc bảo trì phục hình và bệnh nhân được phát bảng câu hỏi hồ sơ tác động sức khỏe răng miệng-phiên bản tiếng Thổ Nhĩ Kỳ 14 (OHIP-TR-14). Sức khỏe mô quanh implant được đánh giá lâm sàng về điểm số mảng bám, điểm số chảy máu, độ sâu thăm dò và chỉ số nướu. Đánh giá X-quang được thực hiện về tình trạng mất xương thẳng đứng quanh implant. Đánh giá X-quang được thực hiện sau 5 năm lắp hàm giả. Không có sự khác biệt có ý nghĩa thống kê giữa các nhóm về tổng điểm OHIP-TR-14 (P > 0,05). Mặc dù tình trạng tiêu xương thẳng đứng trung bình được đo ở nhóm 2 sau 5 năm là 1,38 mm, nhưng có tình trạng mất xương thẳng đứng trung bình là 1,45 mm ở nhóm 3, nhưng sự khác biệt này không có ý nghĩa thống kê (P > 0,05). Trong số các chỉ số sức khỏe nha chu, không có sự khác biệt có ý nghĩa thống kê về giá trị trung bình của các chỉ số nha chu. Theo kết quả nghiên cứu, không có sự khác biệt về tình trạng mất xương thẳng đứng quanh implant ở nhóm 2 và 3, về sức khỏe nha chu ở hàm giả trên hàm dưới có nhóm 2 và 3 hoặc giữa tổng điểm OHIP-TR-14 của bệnh nhân trong tất cả các nhóm."} +{"idx": 1870540, "en": "OBJECTIVE To propose alternative learning strategies for glossopharyngeal breathing in patients with Duchenne muscular dystrophy (DMD) and healthy men. DESIGN A feasibility study with small case series. SUBJECTS Five boys with DMD and 7 male physical therapists as healthy controls who had not learned glossopharyngeal breathing. METHODS Participants were instructed in a glossopharyngeal breathing protocol, including induction methods comprising sucking motions and phonation with inhalation. The protocol consisted of 1-6 sessions (10-15 min each; total 60 min). Criteria for glossopharyngeal breathing mastery were vital capacity with glossopharyngeal insufflation (VCGI)/VC ratio > 1.10 for the DMD group and > 1.05 for the Healthy group. Feasibility outcomes were time required for mastering glossopharyngeal breathing, self-reported outcomes, adverse events and drop-outs. RESULTS All participants learned glossopharyngeal breathing within the allocated 60 min. Mean VCGI/VC ratio was 1.31 for the DMD group and 1.09 for the Healthy group. No adverse events or drop-outs were encountered during the protocol. In most cases, self-reported outcomes showed that motivation increased and difficulty decreased. CONCLUSION Induction methods for sucking motions and phonation with inhalation for glossopharyngeal breathing learning are feasible. This paper proposes alternative strategies for glossopharyngeal breathing learning in boys with DMD and their instructors.", "gemini": "MỤC TIÊU Đề xuất các chiến lược học tập thay thế để thở bằng cơ hầu họng ở những bệnh nhân mắc chứng loạn dưỡng cơ Duchenne (DMD) và những người đàn ông khỏe mạnh. THIẾT KẾ Một nghiên cứu khả thi với loạt ca bệnh nhỏ. ĐỐI TƯỢNG Năm bé trai mắc DMD và 7 nam vật lý trị liệu là nhóm đối chứng khỏe mạnh chưa từng học cách thở bằng cơ hầu họng. PHƯƠNG PHÁP Người tham gia được hướng dẫn theo một giao thức thở bằng cơ hầu họng, bao gồm các phương pháp gây mê bao gồm các động tác mút và phát âm khi hít vào. Giao thức bao gồm 1-6 buổi (mỗi buổi 10-15 phút; tổng cộng 60 phút). Tiêu chuẩn để thành thạo kỹ thuật thở bằng cơ hầu họng là dung tích sống với tỷ lệ thổi phồng bằng cơ hầu họng (VCGI)/VC > 1,10 đối với nhóm DMD và > 1,05 đối với nhóm khỏe mạnh. Kết quả khả thi là thời gian cần thiết để thành thạo kỹ thuật thở bằng cơ hầu họng, kết quả tự báo cáo, các tác dụng phụ và tỷ lệ bỏ cuộc. KẾT QUẢ Tất cả những người tham gia đều học được cách thở bằng cơ hầu họng trong vòng 60 phút được phân bổ. Tỷ lệ VCGI/VC trung bình là 1,31 đối với nhóm DMD và 1,09 đối với nhóm khỏe mạnh. Không có tác dụng phụ hoặc trường hợp bỏ cuộc nào xảy ra trong quá trình thực hiện giao thức. Trong hầu hết các trường hợp, kết quả tự báo cáo cho thấy động lực tăng lên và khó khăn giảm đi. KẾT LUẬN Các phương pháp gây mê để mút và phát âm khi hít vào để học cách thở bằng cơ hầu họng là khả thi. Bài báo này đề xuất các chiến lược thay thế để học cách thở bằng cơ hầu họng ở những bé trai mắc DMD và người hướng dẫn của các bé."} +{"idx": 1870541, "en": "OBJECTIVE To determine the effects of isokinetic training of the knee muscles on bone morphogenetic proteins and inflammatory biomarkers in post-traumatic osteoarthritis after anterior cruciate ligament injury in university football players. METHODS A total of 60 participants with post-traumatic osteoarthritis after anterior cruciate ligament injury were randomly allocated into 3 groups: isokinetic training (n = 20), sensory motor training (n = 20) and control (n = 20) groups. The groups underwent different training programmes for 4 weeks. Clinical and biochemical values were measured at baseline, 4-week, 8-week and 6-month follow-ups. RESULTS Four weeks after training the isokinetic group showed more significant changes in pain intensity and functional disability than the sensory motor training or control groups (p < 0.001). There was no significant improvement in bone morphogenic protein measures, (e.g. bone morphogenic proteins 2, 4, 6, and 7) in any of the groups. There was an improvement in inflammatory markers (CRP, TNF-α, IL-2, IL-4, IL-6) in the isokinetic training group compared with the other 2 groups (p < 0.001). CONCLUSION Isokinetic training results in greater improvements in pain and functional disability than sensory motor training in post-traumatic osteoarthritis after anterior cruciate ligament injury in university football players. The isokinetic training programme had a beneficial effect on levels of inflammatory biomarkers and negligible effect on bone morphogenic proteins.", "gemini": "MỤC TIÊU Xác định tác động của việc tập luyện đẳng động các cơ gối lên protein hình thái xương và các dấu ấn sinh học viêm trong bệnh thoái hóa khớp sau chấn thương sau chấn thương dây chằng chéo trước ở cầu thủ bóng đá đại học. PHƯƠNG PHÁP Tổng cộng 60 người tham gia bị thoái hóa khớp sau chấn thương sau chấn thương dây chằng chéo trước được phân ngẫu nhiên vào 3 nhóm: nhóm tập luyện đẳng động (n = 20), nhóm tập luyện vận động cảm giác (n = 20) và nhóm đối chứng (n = 20). Các nhóm đã trải qua các chương trình đào tạo khác nhau trong 4 tuần. Các giá trị lâm sàng và sinh hóa được đo tại thời điểm ban đầu, theo dõi 4 tuần, 8 tuần và 6 tháng. KẾT QUẢ Bốn tuần sau khi tập luyện, nhóm đẳng động cho thấy những thay đổi đáng kể hơn về cường độ đau và tình trạng tàn tật chức năng so với nhóm tập luyện vận động cảm giác hoặc nhóm đối chứng (p < 0,001). Không có sự cải thiện đáng kể nào trong các biện pháp protein hình thái xương (ví dụ: protein hình thái xương 2, 4, 6 và 7) ở bất kỳ nhóm nào. Có sự cải thiện các dấu ấn viêm (CRP, TNF-α, IL-2, IL-4, IL-6) ở nhóm tập luyện đẳng động so với 2 nhóm còn lại (p < 0,001). KẾT LUẬN Tập luyện đẳng động dẫn đến cải thiện nhiều hơn về đau và tình trạng tàn tật chức năng so với tập luyện vận động cảm giác trong bệnh thoái hóa khớp sau chấn thương sau chấn thương dây chằng chéo trước ở cầu thủ bóng đá đại học. Chương trình tập luyện đẳng động có tác dụng có lợi đối với nồng độ các dấu ấn sinh học viêm và tác dụng không đáng kể đối với protein hình thái xương."} +{"idx": 1870542, "en": "Emerging evidence indicates that NLRP3 inflammasome-induced inflammation plays a crucial role in the pathogenesis of depression. Thus, inhibition of NLRP3 inflammasome activation may offer a therapeutic benefit in the treatment of depression. Metformin has been shown to have potential anti-inflammatory activity, but the underlying mechanisms remain obscure. We used a chronic mild stress model of depression and cultured primary macrophage to investigate the effects of metformin on depression and its underlying mechanisms. We demonstrated that metformin alleviated depressive-like behaviors in the chronic mild stress-induced anhedonia model of depression. We further found that metformin significantly suppressed NLRP3 inflammasome activation, subsequent caspase-1 cleavage, and interleukin-1β secretion in both peripheral macrophages and central hippocampus. Our findings reveal that metformin confers an antidepressant effect partly through inhibition of peripheral and central NLRP3 inflammasome activation. In light of metformin favorable properties, it should be evaluated in the treatment of depression and related neurologic disorders characterized by NLRP3 inflammasome activation.", "gemini": "Những bằng chứng mới nổi chỉ ra rằng tình trạng viêm do NLRP3 inflammasome gây ra đóng vai trò quan trọng trong quá trình sinh bệnh của bệnh trầm cảm. Do đó, việc ức chế hoạt hóa NLRP3 inflammasome có thể mang lại lợi ích điều trị trong điều trị bệnh trầm cảm. Metformin đã được chứng minh là có hoạt tính chống viêm tiềm tàng, nhưng cơ chế cơ bản vẫn chưa rõ ràng. Chúng tôi đã sử dụng mô hình căng thẳng nhẹ mãn tính của bệnh trầm cảm và nuôi cấy đại thực bào nguyên phát để nghiên cứu tác dụng của metformin đối với bệnh trầm cảm và cơ chế cơ bản của nó. Chúng tôi đã chứng minh rằng metformin làm giảm các hành vi giống như trầm cảm ở mô hình mất khoái cảm do căng thẳng nhẹ mãn tính gây ra của bệnh trầm cảm. Chúng tôi tiếp tục phát hiện ra rằng metformin ức chế đáng kể hoạt hóa NLRP3 inflammasome, sự phân cắt caspase-1 tiếp theo và tiết interleukin-1β ở cả đại thực bào ngoại vi và vùng đồi hải mã trung tâm. Những phát hiện của chúng tôi cho thấy metformin mang lại tác dụng chống trầm cảm một phần thông qua việc ức chế hoạt hóa NLRP3 inflammasome ở ngoại vi và trung tâm. Theo đặc tính thuận lợi của metformin, nên đánh giá metformin trong điều trị bệnh trầm cảm và các rối loạn thần kinh liên quan có đặc điểm là hoạt hóa NLRP3 inflammasome."} +{"idx": 1870543, "en": "Although L-DOPA revolutionized in the treatment of Parkinson's disease, most patients developed motor complications after several years of treatment. Adjunctive therapy to L-DOPA with drugs related to dopaminergic signaling may reduce its dose without decreasing the therapeutic efficiency and thus ameliorates its adverse effects. It has been shown that 3,4-diaminopyridine (3,4-DAP), a K channel blocker, increased dopamine release from striatal slices by increasing neuronal firing in striatal dopaminergic terminals. The current study investigates whether 3,4-DAP may enhance L-DOPA-induced dopamine (DA) release from striatal slices by increasing neuronal firing in striatal dopaminergic terminals. The effects of L-DOPA and 3,4-DAP on spontaneous DA and DOPAC release were tested in vitro, on acute rat striatal slices prepared from non-treated and 6-hydroxydopamine-pre-treated rats. DA and DOPAC levels were determined by HPLC methods. When 3,4-diaminopyridine was combined with L-DOPA, the observed effect was considerably greater than the increases induced by L-DOPA or 3,4-DAP alone in normoxic and neurodegenerative conditions produced by FeSO4 and 6-hydroxydopamine. Furthermore, L-DOPA plus 3,4-DAP also ameliorated DOPAC levels in neurodegenerative conditions. These data indicate that 3,4 DAP plus L-DOPA activates striatal dopaminergic terminals by increasing the DA release and, thus, could be considered as a promising finding in treatment of acute and chronic injury in dopaminergic neurons.", "gemini": "Mặc dù L-DOPA đã cách mạng hóa trong điều trị bệnh Parkinson, hầu hết bệnh nhân đều phát triển các biến chứng vận động sau nhiều năm điều trị. Liệu pháp bổ trợ cho L-DOPA với các loại thuốc liên quan đến tín hiệu dopaminergic có thể làm giảm liều dùng của thuốc mà không làm giảm hiệu quả điều trị và do đó làm giảm các tác dụng phụ của thuốc. Người ta đã chứng minh rằng 3,4-diaminopyridine (3,4-DAP), một chất chẹn kênh K, làm tăng giải phóng dopamine từ các lát cắt vân não bằng cách tăng hoạt động bắn nơ-ron ở các đầu mút dopaminergic vân não. Nghiên cứu hiện tại điều tra xem 3,4-DAP có thể tăng cường giải phóng dopamine (DA) do L-DOPA gây ra từ các lát cắt vân não bằng cách tăng hoạt động bắn nơ-ron ở các đầu mút dopaminergic vân não hay không. Các tác động của L-DOPA và 3,4-DAP đối với giải phóng DA và DOPAC tự phát đã được thử nghiệm trong ống nghiệm, trên các lát cắt vân não của chuột cấp tính được chuẩn bị từ chuột không được điều trị và chuột được xử lý trước bằng 6-hydroxydopamine. Nồng độ DA và DOPAC được xác định bằng phương pháp HPLC. Khi kết hợp 3,4-diaminopyridine với L-DOPA, tác dụng quan sát được lớn hơn đáng kể so với sự gia tăng gây ra bởi L-DOPA hoặc 3,4-DAP đơn thuần trong điều kiện thiếu oxy và thoái hóa thần kinh do FeSO4 và 6-hydroxydopamine gây ra. Hơn nữa, L-DOPA cộng với 3,4-DAP cũng làm giảm nồng độ DOPAC trong các tình trạng thoái hóa thần kinh. Những dữ liệu này chỉ ra rằng 3,4 DAP cộng với L-DOPA kích hoạt các đầu mút dopaminergic vân não bằng cách tăng giải phóng DA và do đó, có thể được coi là một phát hiện đầy hứa hẹn trong điều trị tổn thương cấp tính và mãn tính ở các tế bào thần kinh dopaminergic."} +{"idx": 1870544, "en": "INTRODUCTION The aim of the present study was to introduce surgical technique using long PFNA for the treatment of ipsilateral intertrochanteric and femoral shaft fractures, and evaluate the characteristics of this fracture by comparing its surgical outcomes with those of isolated intertrochanteric and femoral shaft fractures. MATERIALS AND METHODS Between March 2013 and December 2018, 38 patients with ipsilateral intertrochanteric and femoral shaft fracture were identified at two institutions. Twenty-eight patients with ipsilateral intertrochanteric and femoral shaft fractures were enrolled in the present study. After propensity score matching, fifty-six patients with isolated intertrochanteric (group B) and femoral shaft (group C) fractures were finally enrolled in the present study for 1:2 matching to compare surgical outcomes to that of ipsilateral intertrochanteric and femoral shaft fractures (Group A). RESULTS All 28 patients achieved union of intertrochanteric fractures, while two experienced non-union of femoral shaft fractures. The union time of intertrochanteric fractures in group A was significantly shorter than that in group B. The union time of femoral shaft fractures in group A was significantly longer than that in group C. CONCLUSIONS The surgical treatment of ipsilateral intertrochanteric and femoral shaft fractures using long PFNA was advantageous as it allowed both fractures on the same femur to be fixed in one go and showed good surgical outcomes. However, fixation of femoral shaft fractures might be insufficient depending on the fracture level and configuration, and can be a cause of hypertrophic non-union.", "gemini": "GIỚI THIỆU Mục đích của nghiên cứu hiện tại là giới thiệu kỹ thuật phẫu thuật sử dụng PFNA dài để điều trị gãy liên mấu chuyển và thân xương đùi cùng bên, và đánh giá các đặc điểm của gãy xương này bằng cách so sánh kết quả phẫu thuật của gãy xương với kết quả phẫu thuật của gãy liên mấu chuyển và thân xương đùi riêng lẻ. VẬT LIỆU VÀ PHƯƠNG PHÁP Từ tháng 3 năm 2013 đến tháng 12 năm 2018, 38 bệnh nhân bị gãy liên mấu chuyển và thân xương đùi cùng bên đã được xác định tại hai cơ sở. Hai mươi tám bệnh nhân bị gãy liên mấu chuyển và thân xương đùi cùng bên đã được đưa vào nghiên cứu hiện tại. Sau khi ghép điểm khuynh hướng, năm mươi sáu bệnh nhân bị gãy liên mấu chuyển riêng lẻ (nhóm B) và gãy thân xương đùi (nhóm C) cuối cùng đã được đưa vào nghiên cứu hiện tại để ghép 1:2 để so sánh kết quả phẫu thuật với gãy liên mấu chuyển và thân xương đùi cùng bên (Nhóm A). KẾT QUẢ Cả 28 bệnh nhân đều đạt được sự hợp nhất của các gãy liên mấu chuyển, trong khi hai bệnh nhân bị không liền xương đùi. Thời gian liền xương của gãy liên mấu chuyển ở nhóm A ngắn hơn đáng kể so với nhóm B. Thời gian liền xương của gãy thân xương đùi ở nhóm A dài hơn đáng kể so với nhóm C. KẾT LUẬN Điều trị phẫu thuật gãy liên mấu chuyển và thân xương đùi cùng bên bằng PFNA dài có lợi thế là có thể cố định cả hai gãy xương trên cùng một xương đùi trong một lần và cho thấy kết quả phẫu thuật tốt. Tuy nhiên, việc cố định gãy thân xương đùi có thể không đủ tùy thuộc vào mức độ và cấu hình gãy xương, và có thể là nguyên nhân gây ra tình trạng không liền xương phì đại."} +{"idx": 1870545, "en": "Purple leaves are rich in health-protecting anthocyanins and food colorants in Brassica juncea. But the causal gene, which is related to leaf color formation, have not been reported in B. juncea. Anthocyanins mainly accumulated throughout the adaxial and abaxial epidermal leaf cells of purple leaves. A genetic analysis indicated that an incompletely dominant gene controls the purple leaf trait in B. juncea. Furthermore, the BjPur gene, which increased anthocyanin accumulation in purple-leaf mustard, was cloned. Blast and phylogenetic analyses revealed that BjPur encodes a new R2R3-MYB transcription factor. Sequence analysis of two alleles revealed a DNA sequence insertion in the first intron of BjPur in green leaves parent line (LY) when compared with the BjPur gene in the purple-leaf parent line (ZY). And this insertion greatly reduced the transcription of BjPur in green leaves. In purple-leaf plants, the transcript level of BjPur was significantly higher in leaves than in roots, stems, siliques, and flower buds. Additionally, molecular markers linked to leaf color were developed to distinguish different genotypes of B. juncea. These results will be helpful for the genetic improvement of the purple leaf color in B. juncea.", "gemini": "Lá màu tím rất giàu anthocyanin bảo vệ sức khỏe và chất tạo màu thực phẩm trong cải thìa. Nhưng gen nhân quả, có liên quan đến quá trình hình thành màu lá, vẫn chưa được báo cáo trong B. juncea. Anthocyanin chủ yếu tích tụ trên khắp các tế bào biểu bì lá mặt trên và mặt dưới của lá màu tím. Phân tích di truyền chỉ ra rằng một gen trội không hoàn toàn kiểm soát đặc điểm lá màu tím ở B. juncea. Hơn nữa, gen BjPur, làm tăng sự tích tụ anthocyanin trong cải lá tím, đã được nhân bản. Các phân tích nổ và phát sinh loài cho thấy BjPur mã hóa một yếu tố phiên mã R2R3-MYB mới. Phân tích trình tự của hai alen cho thấy một đoạn chèn trình tự DNA trong intron đầu tiên của BjPur ở dòng bố lá xanh (LY) khi so sánh với gen BjPur ở dòng bố lá tím (ZY). Và sự chèn này làm giảm đáng kể phiên mã của BjPur ở lá xanh. Ở cây lá tím, mức độ phiên mã của BjPur cao hơn đáng kể ở lá so với rễ, thân, quả cải và nụ hoa. Ngoài ra, các dấu hiệu phân tử liên quan đến màu lá đã được phát triển để phân biệt các kiểu gen khác nhau của B. juncea. Những kết quả này sẽ hữu ích cho việc cải thiện di truyền màu lá tím ở B. juncea."} +{"idx": 1870546, "en": "PURPOSE Although 5-aminosalicylates and thiopurines may have an antineoplastic effect on colorectal neoplasia in patients with inflammatory bowel disease (IBD), their impact on the progression of low-grade dysplasia (LGD) in IBD is uncertain. Therefore, we performed a systematic review and meta-analysis to evaluate whether 5-aminosalicylates or thiopurines can protect against the progression of LGD in patients with IBD. METHODS Systematic searches of PubMed, EMBASE, Cochrane Library databases, and major conference proceedings were conducted to identify all eligible studies through March 2020. Data were pooled using a random effects model. Study quality was assessed using the Newcastle-Ottawa Scale. RESULTS Five studies comprising 776 IBD patients with LGD were included. Overall, 5-aminosalicylates (Hazard ratio (HR) = 0.91, 95% confidence interval (CI) 0.55-1.51) and thiopurines (HR = 0.64, 95% CI 0.23-1.79) did not significantly reduce the risk of advanced colorectal neoplasia (high-grade dysplasia/cancer) in IBD patients with LGD. Moreover, the effects of 5-aminosalicylates or thiopurines on risk of advanced colorectal neoplasia in IBD patients with LGD were not significant by different primary sclerosing cholangitis status, study quality, sample size, and IBD type. CONCLUSIONS In this study, we did not find a significant protective effect of 5-aminosalicylates or thiopurines on the progression of LGD in patients with IBD.", "gemini": "MỤC ĐÍCH Mặc dù 5-aminosalicylate và thiopurine có thể có tác dụng chống tân sinh trên tân sinh đại tràng trực tràng ở những bệnh nhân mắc bệnh viêm ruột (IBD), tác động của chúng lên sự tiến triển của loạn sản độ thấp (LGD) trong IBD vẫn chưa chắc chắn. Do đó, chúng tôi đã tiến hành một đánh giá có hệ thống và phân tích tổng hợp để đánh giá xem 5-aminosalicylate hoặc thiopurine có thể bảo vệ chống lại sự tiến triển của LGD ở những bệnh nhân mắc IBD hay không. PHƯƠNG PHÁP Các tìm kiếm có hệ thống trên PubMed, EMBASE, cơ sở dữ liệu Thư viện Cochrane và biên bản các hội nghị lớn đã được thực hiện để xác định tất cả các nghiên cứu đủ điều kiện cho đến tháng 3 năm 2020. Dữ liệu được tập hợp bằng mô hình hiệu ứng ngẫu nhiên. Chất lượng nghiên cứu được đánh giá bằng Thang điểm Newcastle-Ottawa. KẾT QUẢ Có năm nghiên cứu bao gồm 776 bệnh nhân IBD mắc LGD đã được đưa vào. Nhìn chung, 5-aminosalicylate (Tỷ lệ nguy cơ (HR) = 0,91, Khoảng tin cậy (CI) 95% 0,55-1,51) và thiopurine (HR = 0,64, CI 95% 0,23-1,79) không làm giảm đáng kể nguy cơ tân sinh đại tràng trực tràng tiến triển (loạn sản độ cao/ung thư) ở bệnh nhân IBD mắc LGD. Hơn nữa, tác dụng của 5-aminosalicylate hoặc thiopurine đối với nguy cơ tân sinh đại tràng trực tràng tiến triển ở bệnh nhân IBD mắc LGD không đáng kể theo tình trạng viêm đường mật xơ cứng nguyên phát, chất lượng nghiên cứu, quy mô mẫu và loại IBD khác nhau. KẾT LUẬN Trong nghiên cứu này, chúng tôi không tìm thấy tác dụng bảo vệ đáng kể của 5-aminosalicylate hoặc thiopurine đối với sự tiến triển của LGD ở những bệnh nhân mắc IBD."} +{"idx": 1870547, "en": "BACKGROUND The competencies of advanced practice nurses (APN) include clinical activity in a special field, coaching and consulting, collaboration, leadership, ethical decision-making, and research competence. Clinical leaders initiate and implement changes in the healthcare system and respond to the needs of patients and healthcare institutions. Clinical leadership based on advanced nursing practice can challenge existing management and care structures. OBJECTIVES The aim of the review is to provide an overview of clinical leadership competencies based on advanced nursing practice. The investigated question deals with which roles and competencies of APN are associated with clinical leadership from a national and international perspective. METHODS A systematic search in MEDLINE®(US National Center for Biotechnology Information (NCBI))/PubMed, CINAHL and the Cochrane Library as well as a hand search in library catalogs and journals (April 2019 to January 2020) produced a total of 235 hits. Eight studies were included in the review and analyzed. RESULTS Clinical leadership competencies are increasingly a topic of international studies and can be described and recognized in clinical practice on the basis of leadership models, and the role and leadership domains of APNs. Clinical leadership in nursing practice is recognized when APNs independently control treatment processes in complex nursing situations, exert influence, develop and implement change strategies, consult, coach, train, collaborate, and establish a connection to other health professionals and management. In order for clinical leadership competencies to be effective in nursing care processes, broad support in the multiprofessional team, structural support of the organization and legal legitimation are required. CONCLUSIONS There are hardly any studies in the German-language literature on the relevance, interpretation, and legitimacy of APN clinical leadership. Further research is needed on the interpretation of the roles of APNs, especially clinical leadership competencies, their influence on nursing care processes, leadership structures, and their interaction within the organizational culture.", "gemini": "BỐI CẢNH Năng lực của điều dưỡng hành nghề nâng cao (APN) bao gồm hoạt động lâm sàng trong một chuyên ngành, hướng dẫn và tư vấn, hợp tác, lãnh đạo, ra quyết định đạo đức và năng lực nghiên cứu. Các nhà lãnh đạo lâm sàng khởi xướng và thực hiện những thay đổi trong hệ thống chăm sóc sức khỏe và đáp ứng nhu cầu của bệnh nhân và các tổ chức chăm sóc sức khỏe. Lãnh đạo lâm sàng dựa trên hành nghề điều dưỡng nâng cao có thể thách thức các cấu trúc quản lý và chăm sóc hiện có. MỤC TIÊU Mục đích của đánh giá là cung cấp cái nhìn tổng quan về năng lực lãnh đạo lâm sàng dựa trên hành nghề điều dưỡng nâng cao. Câu hỏi được điều tra liên quan đến vai trò và năng lực nào của APN có liên quan đến lãnh đạo lâm sàng theo quan điểm quốc gia và quốc tế. PHƯƠNG PHÁP Tìm kiếm có hệ thống trong MEDLINE® (Trung tâm Thông tin Công nghệ sinh học Quốc gia Hoa Kỳ (NCBI)) / PubMed, CINAHL và Thư viện Cochrane cũng như tìm kiếm thủ công trong danh mục thư viện và tạp chí (tháng 4 năm 2019 đến tháng 1 năm 2020) đã cho tổng cộng 235 kết quả. Tám nghiên cứu đã được đưa vào đánh giá và phân tích. KẾT QUẢ Năng lực lãnh đạo lâm sàng ngày càng trở thành chủ đề của các nghiên cứu quốc tế và có thể được mô tả và nhận ra trong thực hành lâm sàng trên cơ sở các mô hình lãnh đạo và vai trò và phạm vi lãnh đạo của APN. Lãnh đạo lâm sàng trong thực hành điều dưỡng được công nhận khi APN kiểm soát độc lập các quy trình điều trị trong các tình huống điều dưỡng phức tạp, gây ảnh hưởng, phát triển và thực hiện các chiến lược thay đổi, tham vấn, hướng dẫn, đào tạo, hợp tác và thiết lập kết nối với các chuyên gia y tế và quản lý khác. Để năng lực lãnh đạo lâm sàng có hiệu quả trong các quy trình chăm sóc điều dưỡng, cần có sự hỗ trợ rộng rãi trong nhóm đa chuyên gia, hỗ trợ về mặt cấu trúc của tổ chức và hợp pháp hóa về mặt pháp lý. KẾT LUẬN Hầu như không có nghiên cứu nào trong tài liệu tiếng Đức về tính phù hợp, cách giải thích và tính hợp pháp của APN lãnh đạo lâm sàng. Cần nghiên cứu thêm về cách giải thích vai trò của APN, đặc biệt là năng lực lãnh đạo lâm sàng, ảnh hưởng của họ đối với các quy trình chăm sóc điều dưỡng, cấu trúc lãnh đạo và sự tương tác của họ trong văn hóa tổ chức."} +{"idx": 1870548, "en": "The heavy metals were studied in water, sediments, algae, and various tissues of Glyptosternon reticulatum and Cyprinus carpio from River Swat, Pakistan, using flame atomic absorption spectrophotometer. The Zn, Cu, Pb and Ni were higher in water at sewage site compared to upstream and downstream sites. In sediments, the Ni and Cd were not detected whereas Cu, Pb and Zn were higher at downstream followed by sewage and upstream sites. The Ni and Zn in algae were higher at upstream and sewage sites compared to downstream site whereas Pb and Cd were higher at upstream site compared to sewage and downstream sites and Cu was found same at all the three sites. The heavy metals (Zn > Cu > Pb and Ni) in tissues (liver > gills > skin > muscles) of G. reticulatum was higher than in C. carpio. This study recommends the proper monitoring of River Swat in order to save its water and inhabitant aquatic life.", "gemini": "Các kim loại nặng đã được nghiên cứu trong nước, trầm tích, tảo và các mô khác nhau của Glyptosternon reticulatum và Cyprinus carpio từ Sông Swat, Pakistan, bằng máy quang phổ hấp thụ nguyên tử ngọn lửa. Zn, Cu, Pb và Ni cao hơn trong nước tại địa điểm nước thải so với các địa điểm thượng nguồn và hạ nguồn. Trong trầm tích, không phát hiện thấy Ni và Cd trong khi Cu, Pb và Zn cao hơn ở hạ nguồn tiếp theo là nước thải và các địa điểm thượng nguồn. Ni và Zn trong tảo cao hơn ở các địa điểm thượng nguồn và nước thải so với địa điểm hạ nguồn trong khi Pb và Cd cao hơn ở địa điểm thượng nguồn so với các địa điểm nước thải và hạ nguồn và Cu được tìm thấy giống nhau ở cả ba địa điểm. Các kim loại nặng (Zn> Cu> Pb và Ni) trong các mô (gan> mang> da> cơ) của G. reticulatum cao hơn ở C. carpio. Nghiên cứu này khuyến nghị theo dõi đúng cách Sông Swat để bảo vệ nguồn nước và sinh vật thủy sinh cư trú của nó."} +{"idx": 1870549, "en": "Biosorption has gained increased attention as a reliable and proven technology for the remediation of industrial effluents rich in chromium. The present study was planned to isolate potential fungi from effluents contaminated sites and assess their efficiency for the absorption and reduction of chromium. Two species of Aspergillus and a species of Trichoderma which were isolated from contaminated sites and exhibited resistance to 10 mM of chromium on agar were chosen for the study. A biosorbent was designed by growing these fungal isolates on luffa sponge under shaken condition. The absorption and reduction of chromium, by the designed biosorbent was determined by Atomic Absorption Spectrophotometry and UV Visible Spectrophotometer. Actively growing fungi on luffa sponge showed better absorption (21%-25%) and reduction (28%-35%) capacity when compared to heat killed biosorbent in all fungi tested within 24 h of incubation. Interestingly, there was a liner increase in the absorption and reduction (85%-100%) of chromium by the biosorbent designed by using A. niger.", "gemini": "Sinh hấp phụ đã nhận được sự chú ý ngày càng tăng như một công nghệ đáng tin cậy và đã được chứng minh để khắc phục các chất thải công nghiệp giàu crom. Nghiên cứu hiện tại được lập kế hoạch để phân lập các loại nấm tiềm năng từ các địa điểm bị ô nhiễm và đánh giá hiệu quả của chúng đối với quá trình hấp thụ và khử crom. Hai loài Aspergillus và một loài Trichoderma được phân lập từ các địa điểm bị ô nhiễm và biểu hiện khả năng kháng 10 mM crom trên thạch đã được chọn để nghiên cứu. Một chất hấp phụ sinh học được thiết kế bằng cách nuôi các chủng nấm này trên miếng bọt biển luffa trong điều kiện lắc. Quá trình hấp thụ và khử crom, do chất hấp phụ sinh học được thiết kế xác định bằng phương pháp quang phổ hấp thụ nguyên tử và quang phổ hấp thụ tử ngoại khả kiến. Nấm phát triển mạnh trên miếng bọt biển luffa cho thấy khả năng hấp thụ (21% -25%) và khử (28% -35%) tốt hơn khi so sánh với chất hấp phụ sinh học đã bị nhiệt tiêu diệt ở tất cả các loại nấm được thử nghiệm trong vòng 24 giờ ủ. Điều thú vị là có sự gia tăng tuyến tính trong quá trình hấp thụ và khử (85% -100%) crom bởi chất hấp phụ sinh học được thiết kế bằng cách sử dụng A. niger."} +{"idx": 1870550, "en": "Chromate is considered as a serious environmental problem due its toxicity. Iron nanoparticles produced by green tea polyphenols (GT-nZVI) is a powerful reductant, which can effectively reduce Cr(VI) to Cr(III). Nano ZVI suspension was initially conceived ideal for direct injection in the contaminated aquifers. However GT-nZVI presents limited mobility in calcareous aquifers. For this reason the incorporation of nanoiron in a permeable reactive barrier was investigated as an alternative mode of GT-nZVI application. Namely an amount of soil was loaded with nZVI (0.40 mmol/g of soil) and was evaluated for Cr(VI) removal by conducting batch and column tests. Batch tests were carried out by mixing soil samples, loaded with different levels of nZVI from 0.04 to 0.40 mmol per gram, with contaminated groundwater (GW) containing 1300 ppb Cr(VI). Cr(VI) concentration dropped below detection limit within 1 day using the highest nZVI dose. Soil pre-loaded with nZVI (S-nZVI) presented also high efficiency for chromates remediation, when tested under flow conditions by conducting column tests.", "gemini": "Cromat được coi là một vấn đề nghiêm trọng về môi trường do độc tính của nó. Các hạt nano sắt được sản xuất bởi polyphenol trà xanh (GT-nZVI) là chất khử mạnh, có thể khử hiệu quả Cr(VI) thành Cr(III). Hỗn dịch Nano ZVI ban đầu được coi là lý tưởng để tiêm trực tiếp vào các tầng chứa nước bị ô nhiễm. Tuy nhiên GT-nZVI có tính di động hạn chế trong các tầng chứa nước có tính chất vôi. Vì lý do này, việc kết hợp nanoiron trong một rào cản phản ứng thấm đã được nghiên cứu như một phương thức thay thế để ứng dụng GT-nZVI. Cụ thể, một lượng đất đã được nạp nZVI (0,40 mmol/g đất) và được đánh giá khả năng loại bỏ Cr(VI) bằng cách tiến hành các thử nghiệm mẻ và cột. Các thử nghiệm mẻ được thực hiện bằng cách trộn các mẫu đất, được nạp các mức nZVI khác nhau từ 0,04 đến 0,40 mmol trên một gam, với nước ngầm bị ô nhiễm (GW) chứa 1300 ppb Cr(VI). Nồng độ Cr(VI) giảm xuống dưới giới hạn phát hiện trong vòng 1 ngày khi sử dụng liều nZVI cao nhất. Đất được tải trước với nZVI (S-nZVI) cũng cho thấy hiệu quả cao trong việc khắc phục cromat, khi được thử nghiệm trong điều kiện dòng chảy bằng cách tiến hành các thử nghiệm cột."} +{"idx": 1870551, "en": "PURPOSE Enhanced Recovery after Surgery has been proven effective for patients with gastrointestinal cancer. But radical enhanced recovery could also lead to adverse clinical outcomes. Compared with reports on the estimation of successful implementation of enhanced recovery, studies on risk factors of enhanced recovery failure are still lacking. METHODS A retrospective analysis was carried out on 102 patients in ERAS who underwent elective colon cancer surgery. This study included 102 patients with colon cancer between 2015 and 2019, defining enhanced recovery failure as postoperative length of stay over 10 days, stay in ICU over 24 h after surgery, reoperation, death, or unplanned readmission within 30 days after surgery. Univariate and multivariate analyses were performed to explore potential risk factors of failure. RESULTS Aged ≥ 75, open operation, number of drainage tube over 1, re-urethral catheterization, and Clavien-Dindo grade over 2 were associated with ERAS failure, according to univariate analysis. Multivariate analysis showed that age ≥ 75 [OR 7.231; P = 0.009]; open operation (OR 3.599; P = 0.021); and number of drainage tube over 1 (OR 3.202; P = 0.020) were independent risk factors for ERAS failure. CONCLUSIONS We found age ≥ 75, open operation, and number of drainage tube over 1 are independent risk factors associated with ERAS failure after colon cancer surgery.", "gemini": "MỤC ĐÍCH Phục hồi nâng cao sau phẫu thuật đã được chứng minh là có hiệu quả đối với bệnh nhân ung thư đường tiêu hóa. Nhưng phục hồi nâng cao triệt để cũng có thể dẫn đến các kết cục lâm sàng bất lợi. So với các báo cáo về ước tính triển khai thành công phục hồi nâng cao, các nghiên cứu về các yếu tố rủi ro của việc phục hồi nâng cao không thành công vẫn còn thiếu. PHƯƠNG PHÁP Một phân tích hồi cứu đã được thực hiện trên 102 bệnh nhân trong ERAS đã trải qua phẫu thuật ung thư đại tràng theo kế hoạch. Nghiên cứu này bao gồm 102 bệnh nhân ung thư đại tràng trong giai đoạn 2015-2019, định nghĩa phục hồi nâng cao không thành công là thời gian nằm viện sau phẫu thuật trên 10 ngày, nằm ở ICU trên 24 giờ sau phẫu thuật, phẫu thuật lại, tử vong hoặc nhập viện lại không theo kế hoạch trong vòng 30 ngày sau phẫu thuật. Phân tích đơn biến và đa biến đã được thực hiện để khám phá các yếu tố rủi ro tiềm ẩn của việc không thành công. KẾT QUẢ Theo phân tích đơn biến, tuổi ≥ 75, phẫu thuật mở, số ống dẫn lưu trên 1, đặt lại ống thông niệu đạo và độ Clavien-Dindo trên 2 có liên quan đến việc ERAS không thành công. Phân tích đa biến cho thấy tuổi ≥ 75 [OR 7,231; P = 0,009]; phẫu thuật mở (OR 3,599; P = 0,021); và số ống dẫn lưu trên 1 (OR 3,202; P = 0,020) là các yếu tố nguy cơ độc lập dẫn đến việc ERAS không thành công. KẾT LUẬN Chúng tôi thấy rằng tuổi ≥ 75, phẫu thuật mở và số ống dẫn lưu trên 1 là các yếu tố nguy cơ độc lập liên quan đến việc ERAS không thành công sau phẫu thuật ung thư đại tràng."} +{"idx": 1870552, "en": "Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) is a dual kinase that can phosphorylate its own activation loop on tyrosine residue and phosphorylate its substrates on threonine and serine residues. It is the most studied member of DYRK kinases, because its gene maps to human chromosome 21 within the Down syndrome critical region (DSCR). DYRK1A overexpression was found to be responsible for the phenotypic features observed in Down syndrome such as mental retardation, early onset neurodegenerative, and developmental heart defects. Besides its dual activity in phosphorylation, DYRK1A carries the characteristic of duality in tumorigenesis. Many studies indicate its possible role as a tumor suppressor gene; however, others prove its pro-oncogenic activity. In this review, we will focus on its multifaceted role in tumorigenesis by explaining its participation in some cancer hallmarks pathways such as proliferative signaling, transcription, stress, DNA damage repair, apoptosis, and angiogenesis, and finally, we will discuss targeting DYRK1A as a potential strategy for management of cancer and neurodegenerative disorders.", "gemini": "Kinase 1A điều hòa phosphoryl hóa tyrosine có tính đặc hiệu kép (DYRK1A) là một kinase kép có thể phosphoryl hóa vòng hoạt hóa của chính nó trên gốc tyrosine và phosphoryl hóa các chất nền của nó trên các gốc threonine và serine. Đây là thành viên được nghiên cứu nhiều nhất của kinase DYRK, vì gen của nó được lập bản đồ trên nhiễm sắc thể 21 của người trong vùng quan trọng của hội chứng Down (DSCR). Sự biểu hiện quá mức của DYRK1A được phát hiện là nguyên nhân gây ra các đặc điểm kiểu hình quan sát thấy ở hội chứng Down như chậm phát triển trí tuệ, thoái hóa thần kinh khởi phát sớm và các khuyết tật tim phát triển. Bên cạnh hoạt động kép trong quá trình phosphoryl hóa, DYRK1A mang đặc điểm kép trong quá trình hình thành khối u. Nhiều nghiên cứu chỉ ra vai trò có thể có của nó như một gen ức chế khối u; tuy nhiên, những nghiên cứu khác chứng minh hoạt động gây ung thư của nó. Trong bài đánh giá này, chúng tôi sẽ tập trung vào vai trò nhiều mặt của nó trong quá trình hình thành khối u bằng cách giải thích sự tham gia của nó vào một số con đường đặc trưng của bệnh ung thư như tín hiệu tăng sinh, phiên mã, căng thẳng, sửa chữa tổn thương DNA, apoptosis và hình thành mạch máu, và cuối cùng, chúng tôi sẽ thảo luận về việc nhắm mục tiêu DYRK1A như một chiến lược tiềm năng để quản lý ung thư và các rối loạn thoái hóa thần kinh."} +{"idx": 1870553, "en": "The original published online version contains mistake in the Title and in Abstract section.", "gemini": "Phiên bản trực tuyến được xuất bản ban đầu có lỗi trong Tiêu đề và phần Tóm tắt."} +{"idx": 1870554, "en": "Corneal changes associated with eyelid disease are frequent and can considerably complicate the course of the disease. They can manifest as refraction anomalies, corneal degeneration, ocular surface disease, corneal infiltrates, corneal vascularization and corneal ulceration up to corneal perforation. Pathophysiologically relevant are compressive forces, exposure, trichiasis and distichiasis, eye rubbing, inflammation and infection. A multitude of eyelid diseases is associated with diseases of the cornea including eyelid malposition, eyelid tumors, eyelid infections, allergic eyelid dermatitis, blepharitis, meibomitis, and Meibomian gland disease.", "gemini": "Những thay đổi ở giác mạc liên quan đến bệnh mí mắt thường gặp và có thể làm phức tạp đáng kể quá trình của bệnh. Chúng có thể biểu hiện dưới dạng bất thường khúc xạ, thoái hóa giác mạc, bệnh bề mặt nhãn cầu, thâm nhiễm giác mạc, mạch máu hóa giác mạc và loét giác mạc cho đến thủng giác mạc. Về mặt bệnh sinh lý, các lực nén, tiếp xúc, lông quặm và lông mi đôi, dụi mắt, viêm và nhiễm trùng có liên quan. Rất nhiều bệnh về mí mắt liên quan đến các bệnh về giác mạc bao gồm lệch lạc mí mắt, u mí mắt, nhiễm trùng mí mắt, viêm da mí mắt dị ứng, viêm bờ mi, viêm tuyến meibom và bệnh tuyến meibom."} +{"idx": 1870555, "en": "We evaluated the ventilation ability of two types (plug-type and cap-type) of culture-stoppers having standard air permeability. The culture-stoppers were evaluated using the circulation direct monitoring and sampling system with CO2 concentration in the gas phase of a shake-flask culture as an index. The half-lives of CO2 in the headspace of the shake flask with the plug-type and cap-type stoppers were about 51.5 min and about 30.3 min, respectively. Based on these half-lives, we formulated a model equation to simulate the behaviour of CO2 with different culture-stoppers. After validating the model equation by shake-flask culture with Saccharomyces cerevisiae, we investigated the effect of different ventilation abilities of the culture-stoppers on the growth of Pelomonas saccharophila and Escherichia coli: the sensitivity of the culture-stopper to the ventilation ability was dependent on the microorganism species. In the case of P. saccharophila, when the plug-type culture-stopper was combined with controlled CO2 concentration (6%) in the flask, the maximum yield increased by twofold compared to that of the control. This study shows the importance of ventilation in headspace and conventional culture-stoppers during the shake-flask culture of microorganisms. The problems that may occur between the conventional shake-flask culture approach using a breathable culture-stopper and the next-generation shake-flask culture without a conventional culture-stopper were clarified from the evaluation of gas-permeable culture-stoppers. The importance of controlled gaseous phase in the headspace during shake-flask culture of the microorganisms was also elucidated. KEY POINTS: • Ventilation capacity of culture-stoppers was evaluated using the CO2 half-life concentration. • Behaviour of microorganisms varies with the type of culture-stopper. • Developed a PID system for control of CO2 in flask gas phase to enhance the shake-flask culture.", "gemini": "Chúng tôi đã đánh giá khả năng thông khí của hai loại (loại phích cắm và loại nắp) nút chặn nuôi cấy có khả năng thấm khí tiêu chuẩn. Các nút chặn nuôi cấy được đánh giá bằng hệ thống giám sát trực tiếp tuần hoàn và lấy mẫu với nồng độ CO2 trong pha khí của nuôi cấy bình lắc như một chỉ số. Thời gian bán hủy của CO2 trong khoảng trống của bình lắc có nút chặn loại phích cắm và loại nắp lần lượt là khoảng 51,5 phút và khoảng 30,3 phút. Dựa trên các thời gian bán hủy này, chúng tôi đã xây dựng một phương trình mô hình để mô phỏng hành vi của CO2 với các nút chặn nuôi cấy khác nhau. Sau khi xác nhận phương trình mô hình bằng nuôi cấy bình lắc với Saccharomyces cerevisiae, chúng tôi đã nghiên cứu tác động của các khả năng thông khí khác nhau của các nút chặn nuôi cấy đến sự phát triển của Pelomonas saccharophila và Escherichia coli: độ nhạy của nút chặn nuôi cấy đối với khả năng thông khí phụ thuộc vào loài vi sinh vật. Trong trường hợp của P. saccharophila, khi nút chặn nuôi cấy loại phích cắm được kết hợp với nồng độ CO2 được kiểm soát (6%) trong bình, năng suất tối đa tăng gấp đôi so với đối chứng. Nghiên cứu này cho thấy tầm quan trọng của thông khí trong khoảng trống và các nút chặn nuôi cấy thông thường trong quá trình nuôi cấy bình lắc các vi sinh vật. Các vấn đề có thể xảy ra giữa phương pháp nuôi cấy bình lắc thông thường sử dụng nút chặn nuôi cấy có thể thở được và nuôi cấy bình lắc thế hệ tiếp theo không có nút chặn nuôi cấy thông thường đã được làm rõ từ việc đánh giá các nút chặn nuôi cấy thấm khí. Tầm quan trọng của pha khí được kiểm soát trong khoảng trống trong quá trình nuôi cấy bình lắc các vi sinh vật cũng đã được làm sáng tỏ. CÁC ĐIỂM CHÍNH: • Khả năng thông khí của các nút chặn nuôi cấy được đánh giá bằng nồng độ thời gian bán hủy CO2. • Hành vi của vi sinh vật thay đổi tùy theo loại nút chặn nuôi cấy. • Phát triển một hệ thống PID để kiểm soát CO2 trong pha khí bình để tăng cường nuôi cấy bình lắc."} +{"idx": 1870556, "en": "INTRODUCTION AND HYPOTHESIS This study synthesized the effects of unsupervised behavioral and pelvic floor muscle training (B-PFMT) programs on outcomes relevant to women's storage lower urinary tract symptoms (LUTS) and pelvic floor muscle strength (PFMS). METHODS PubMed, CINAHL, Web of Science, and PsycINFO were searched since their inception to August 6, 2019. Randomized controlled trials (RCTs) and quasi-experimental articles that enrolled community women aged 18 years and older and reported storage LUTS outcomes including symptoms, severity, impact, self-reported symptom improvement, and PFMS were screened and extracted. Risk of bias was evaluated, and a narrative synthesis approach was used to synthesize evidence. RESULTS Ten RCTs and three pretest-posttest articles were retrieved. Half of the RCTs had some concerns of bias; the remaining RCTs had high risk of bias. Three pretest-posttest articles had at least some risk of bias. Ten articles aimed to treat urinary incontinence (UI) primarily in middle-aged women, two aimed to prevent UI, and one aimed to improve PFMS in young continent women. Two months post-intervention, UI treatment effectiveness was observed, and cumulative effectiveness was evident in: (1) reduction in the number of incontinent episodes, (2) reduction in the International Consultation on Incontinence Questionnaire-Urinary Incontinence-Short Form and International Consultation on Incontinence Questionnaire-Lower Urinary Tract Symptoms Quality of Life scores, (3) increased patients' global impression of improvement, and (4) improvement of PFMS. CONCLUSIONS Unsupervised B-PFMT programs improve outcomes relevant to UI and PFMS in midlife community women who have UI. Their effects on UI prevention and other storage LUTS remain unclear.", "gemini": "GIỚI THIỆU VÀ GIẢ THIẾT Nghiên cứu này tổng hợp các tác động của các chương trình huấn luyện cơ sàn chậu và hành vi không giám sát (B-PFMT) đối với các kết quả có liên quan đến các triệu chứng đường tiết niệu dưới (LUTS) và sức mạnh cơ sàn chậu (PFMS) ở phụ nữ. PHƯƠNG PHÁP PubMed, CINAHL, Web of Science và PsycINFO đã được tìm kiếm từ khi thành lập đến ngày 6 tháng 8 năm 2019. Các thử nghiệm có đối chứng ngẫu nhiên (RCT) và các bài báo bán thực nghiệm tuyển dụng phụ nữ cộng đồng từ 18 tuổi trở lên và báo cáo kết quả LUTS về lưu trữ bao gồm các triệu chứng, mức độ nghiêm trọng, tác động, cải thiện triệu chứng tự báo cáo và PFMS đã được sàng lọc và trích xuất. Rủi ro thiên vị đã được đánh giá và phương pháp tổng hợp tường thuật đã được sử dụng để tổng hợp bằng chứng. KẾT QUẢ Mười RCT và ba bài báo tiền kiểm-hậu kiểm đã được thu hồi. Một nửa trong số các RCT có một số mối quan tâm về thiên vị; các RCT còn lại có nguy cơ thiên vị cao. Ba bài báo tiền kiểm-hậu kiểm có ít nhất một số nguy cơ thiên vị. Mười bài báo nhằm điều trị chứng tiểu không tự chủ (UI) chủ yếu ở phụ nữ trung niên, hai bài nhằm mục đích ngăn ngừa UI và một bài nhằm mục đích cải thiện PFMS ở phụ nữ trẻ bình thường. Hai tháng sau khi can thiệp, hiệu quả điều trị UI đã được quan sát thấy và hiệu quả tích lũy đã được chứng minh ở: (1) giảm số lần són tiểu, (2) giảm điểm số của Phiếu hỏi tham vấn quốc tế về tiểu không tự chủ-Phiếu hỏi ngắn về tiểu không tự chủ và Phiếu hỏi tham vấn quốc tế về tiểu không tự chủ-Chất lượng cuộc sống của các triệu chứng đường tiết niệu dưới, (3) tăng ấn tượng chung của bệnh nhân về sự cải thiện và (4) cải thiện PFMS. KẾT LUẬN Các chương trình B-PFMT không giám sát cải thiện các kết quả có liên quan đến UI và PFMS ở phụ nữ cộng đồng tuổi trung niên bị UI. Tác động của chúng đối với việc ngăn ngừa UI và các LUTS lưu trữ khác vẫn chưa rõ ràng."} +{"idx": 1870557, "en": "INTRODUCTION AND HYPOTHESIS The protocol and analysis methods for the Defining Mechanisms of Anterior Vaginal Wall Descent (DEMAND) study are presented. DEMAND was designed to identify mechanisms and contributors of prolapse recurrence after two transvaginal apical suspension procedures for uterovaginal prolapse. METHODS DEMAND is a supplementary cohort study of a clinical trial in which women with uterovaginal prolapse randomized to (1) vaginal hysterectomy with uterosacral ligament suspension or (2) vaginal mesh hysteropexy underwent pelvic magnetic resonance imaging (MRI) at 30-42 months post-surgery. Standardized protocols have been developed to systematize MRI examinations across multiple sites and to improve reliability of MRI measurements. Anatomical failure, based on MRI, is defined as prolapse beyond the hymen. Anatomic measures from co-registered rest, maximal strain, and post-strain rest (recovery) sequences are obtained from the \"true mid-sagittal\" plane defined by a 3D pelvic coordinate system. The primary outcome is the mechanism of failure (apical descent versus anterior vaginal wall elongation). Secondary outcomes include displacement of the vaginal apex and perineal body and elongation of the anterior wall, posterior wall, perimeter, and introitus of the vagina between (1) rest and strain and (2) rest and recovery. RESULTS Recruitment and MRI trials of 94 participants were completed by May 2018. CONCLUSIONS Methods papers which detail studies designed to evaluate anatomic outcomes of prolapse surgeries are few. We describe a systematic, standardized approach to define and quantitatively assess mechanisms of anatomic failure following prolapse repair. This study will provide a better understanding of how apical prolapse repairs fail anatomically.", "gemini": "GIỚI THIỆU VÀ GIẢ THIẾT Các phương pháp giao thức và phân tích cho nghiên cứu Xác định cơ chế sa thành trước âm đạo (DEMAND) được trình bày. DEMAND được thiết kế để xác định các cơ chế và yếu tố góp phần gây sa tái phát sau hai thủ thuật treo đỉnh ngả âm đạo để sa tử cung âm đạo. PHƯƠNG PHÁP DEMAND là một nghiên cứu nhóm bổ sung của một thử nghiệm lâm sàng trong đó những phụ nữ bị sa tử cung âm đạo được phân ngẫu nhiên thành (1) cắt tử cung qua ngả âm đạo với treo dây chằng tử cung hoặc (2) phẫu thuật treo tử cung bằng lưới qua ngả âm đạo đã trải qua chụp cộng hưởng từ (MRI) vùng chậu tại 30-42 tháng sau phẫu thuật. Các giao thức chuẩn hóa đã được phát triển để hệ thống hóa các xét nghiệm MRI trên nhiều địa điểm và để cải thiện độ tin cậy của các phép đo MRI. Suy giảm giải phẫu, dựa trên MRI, được định nghĩa là sa vượt ra ngoài màng trinh. Các biện pháp giải phẫu từ các chuỗi nghỉ ngơi đồng đăng ký, biến dạng tối đa và nghỉ ngơi sau biến dạng (phục hồi) được lấy từ mặt phẳng \"trung bình thực sự\" được xác định bởi hệ tọa độ vùng chậu 3D. Kết quả chính là cơ chế suy giảm (sa đỉnh so với kéo dài thành trước âm đạo). Các kết quả phụ bao gồm sự di chuyển của đỉnh âm đạo và cơ đáy chậu và sự kéo dài của thành trước, thành sau, chu vi và tiền đình của âm đạo giữa (1) nghỉ ngơi và biến dạng và (2) nghỉ ngơi và phục hồi. KẾT QUẢ Việc tuyển dụng và thử nghiệm MRI của 94 người tham gia đã hoàn thành vào tháng 5 năm 2018. KẾT LUẬN Các bài báo về phương pháp mô tả chi tiết các nghiên cứu được thiết kế để đánh giá kết quả giải phẫu của các ca phẫu thuật sa là rất ít. Chúng tôi mô tả một cách tiếp cận có hệ thống, chuẩn hóa để xác định và đánh giá định lượng các cơ chế suy giảm giải phẫu sau khi sửa chữa sa. Nghiên cứu này sẽ cung cấp sự hiểu biết tốt hơn về cách sửa chữa sa đỉnh bị suy giảm về mặt giải phẫu như thế nào."} +{"idx": 1870558, "en": "INTRODUCTION AND HYPOTHESIS Pelvic organ prolapse (POP) affects the lives of many people. We aimed to systematically search for, identify and synthesize qualitative research that explores what it is like to live with POP and make this knowledge available for healthcare improvement. METHODS We systematically searched Medline, PsychInfo, Embase and CINAHL, from inception to March 2020, for qualitative research exploring the experience of living with POP. We used meta-ethnography to synthesize findings. This is a conceptual approach to qualitative evidence synthesis. We used the recent guidelines for reporting meta-ethnography. RESULTS We screened 3103 titles and 255 abstracts and included 37 primary studies. These incorporated the experience of 777 women, (aged 18 to 95 years) from a range of countries. We organized 162 ideas into 27 conceptual categories and 10 themes. We developed a conceptual model that helps us to understand the experience of pelvic organ prolapse. This model indicates that (1) the physical losses of POP are intricately linked to loss of identity; (2) women conceptualized POP as part of womanhood, yet also its thief; (3) there is a vicious cycle of taboo, silence and misunderstanding about POP and its treatment; (4) this silence is exacerbated by a feeling that POP is not taken seriously in healthcare. CONCLUSIONS This meta-ethnography helps us to understand the experience of living with a POP. Our model illustrates the complex process of healthcare decision making. Further studies to explore the complexity of decision making from the perspective of patient and health professional are timely.", "gemini": "GIỚI THIỆU VÀ GIẢ THUYẾT Sa sút cơ quan vùng chậu (POP) ảnh hưởng đến cuộc sống của nhiều người. Chúng tôi đặt mục tiêu tìm kiếm, xác định và tổng hợp một cách có hệ thống các nghiên cứu định tính khám phá cuộc sống khi mắc POP và biến kiến thức này thành kiến thức có sẵn để cải thiện chăm sóc sức khỏe. PHƯƠNG PHÁP Chúng tôi đã tìm kiếm một cách có hệ thống trên Medline, PsychInfo, Embase và CINAHL, từ khi bắt đầu đến tháng 3 năm 2020, để tìm kiếm các nghiên cứu định tính khám phá kinh nghiệm sống với POP. Chúng tôi đã sử dụng siêu dân tộc học để tổng hợp các phát hiện. Đây là một phương pháp tiếp cận khái niệm để tổng hợp bằng chứng định tính. Chúng tôi đã sử dụng các hướng dẫn gần đây để báo cáo siêu dân tộc học. KẾT QUẢ Chúng tôi đã sàng lọc 3103 tiêu đề và 255 bản tóm tắt và đưa vào 37 nghiên cứu chính. Những nghiên cứu này kết hợp kinh nghiệm của 777 phụ nữ (tuổi từ 18 đến 95) từ nhiều quốc gia. Chúng tôi đã sắp xếp 162 ý tưởng thành 27 danh mục khái niệm và 10 chủ đề. Chúng tôi đã phát triển một mô hình khái niệm giúp chúng tôi hiểu được trải nghiệm về sa sút cơ quan vùng chậu. Mô hình này chỉ ra rằng (1) mất mát về thể chất do POP có liên quan chặt chẽ đến mất nhận dạng; (2) phụ nữ quan niệm POP là một phần của nữ tính, nhưng cũng là kẻ đánh cắp nữ tính; (3) có một vòng luẩn quẩn của sự cấm kỵ, im lặng và hiểu lầm về POP và cách điều trị; (4) sự im lặng này càng trầm trọng hơn bởi cảm giác rằng POP không được coi trọng trong chăm sóc sức khỏe. KẾT LUẬN Siêu dân tộc học này giúp chúng tôi hiểu được trải nghiệm sống chung với POP. Mô hình của chúng tôi minh họa quá trình phức tạp của việc ra quyết định chăm sóc sức khỏe. Các nghiên cứu sâu hơn để khám phá sự phức tạp của việc ra quyết định từ góc độ của bệnh nhân và chuyên gia y tế là rất kịp thời."} +{"idx": 1870559, "en": "PURPOSE The purpose of this study was to present the functional, radiological, and quality of life outcomes of a series of stage III adult-acquired flatfoot deformity corrections using an original operative approach based on minimal incision surgery (MIS). METHODS Sixty-two patients (67 feet) with a symptomatic stage III flatfoot deformity were treated using a modified double arthrodesis by MIS. The mean age was 63 years (range, 50 to 81) and the mean follow-up was 6.6 years (range, 3.2 to 11.5). Clinical, radiological, American Orthopaedic Foot and Ankle Society Hindfoot score (AOFAS score), quality of life (SF-36), and satisfaction scores were collected retrospectively. RESULTS The mean AOFAS score improved by 54.27 (95% [CI], 57.27-51.3; P < 0.0001), and the SF-36 improved by a mean of 17.5 points (95% CI, 1.4-20.9) in the physical component summary (PCS). Deformity correction was confirmed by a significant improvement in the x-ray measurements (six angles). Bony union was observed in 89.5% of cases (60/67). In all, nine patients (13.4%) needed a secondary surgery: three for talonavicular nonunion, four for progression of the flatfoot deformity, and two for screw protrusion. No cases of superficial infection, wound dehiscence, or avascular necrosis of the talus were observed. CONCLUSION The present series represents the largest study of stage III flatfoot correction using MIS with a mid- to long-term follow-up. Because the data showed clinical and functional improvement after surgery with fewer complications, this technique may be ideal for patients at risk of complications. Graphical abstract.", "gemini": "MỤC ĐÍCH Mục đích của nghiên cứu này là trình bày các kết quả chức năng, X quang và chất lượng cuộc sống của một loạt các ca chỉnh sửa dị tật bàn chân bẹt ở người lớn giai đoạn III bằng cách sử dụng phương pháp phẫu thuật ban đầu dựa trên phẫu thuật ít xâm lấn (MIS). PHƯƠNG PHÁP Sáu mươi hai bệnh nhân (67 bàn chân) bị dị tật bàn chân bẹt giai đoạn III có triệu chứng đã được điều trị bằng phương pháp cố định khớp đôi đã sửa đổi bằng MIS. Độ tuổi trung bình là 63 tuổi (khoảng từ 50 đến 81) và thời gian theo dõi trung bình là 6,6 năm (khoảng từ 3,2 đến 11,5). Điểm chân sau của Hiệp hội Chỉnh hình Cổ chân và Bàn chân Hoa Kỳ (điểm AOFAS), chất lượng cuộc sống (SF-36) và điểm hài lòng đã được thu thập hồi cứu. KẾT QUẢ Điểm AOFAS trung bình được cải thiện 54,27 (95% [CI], 57,27-51,3; P < 0,0001) và SF-36 được cải thiện trung bình 17,5 điểm (95% CI, 1,4-20,9) trong tóm tắt thành phần thể chất (PCS). Việc chỉnh sửa dị tật đã được xác nhận bằng sự cải thiện đáng kể trong các phép đo X quang (sáu góc). Sự kết hợp xương được quan sát thấy ở 89,5% trường hợp (60/67). Có tổng cộng chín bệnh nhân (13,4%) cần phẫu thuật thứ phát: ba bệnh nhân bị không liền xương talonavicular, bốn bệnh nhân bị tiến triển dị tật bàn chân bẹt và hai bệnh nhân bị trồi vít. Không có trường hợp nào bị nhiễm trùng nông, vết thương hở hoặc hoại tử vô mạch xương talus. KẾT LUẬN Loạt nghiên cứu hiện tại đại diện cho nghiên cứu lớn nhất về chỉnh sửa bàn chân bẹt giai đoạn III bằng MIS với thời gian theo dõi trung bình đến dài hạn. Vì dữ liệu cho thấy sự cải thiện về mặt lâm sàng và chức năng sau phẫu thuật với ít biến chứng hơn, nên kỹ thuật này có thể lý tưởng cho những bệnh nhân có nguy cơ biến chứng. Tóm tắt đồ họa."} +{"idx": 1870560, "en": "Analytical ultracentrifugation (AUC) cells use either quartz or sapphire windows as end caps for the cell housing. Current generation sapphire windows are not recommended for absorbance data collection below 235 nm, because the window material shows a precipitous drop in transmittance at low wavelengths due to impurities in the sapphire. Quartz windows can be used below 235 nm as they do not exhibit adverse transmittance at low wavelengths. In this study, we demonstrate the optical properties of new generation sapphire windows and compare them to those of quartz windows across a wide range of wavelengths and present the results of sedimentation velocity experiments on BSA using both types of windows using data collected at both the 280 nm absorbance maxima as well as the 230-240 nm (closer to the peptide bond maximum). Our results show that the quartz and new generation sapphire windows deliver identical results in absorbance mode. We also demonstrate that quartz windows suffer significant mechanical deformation while spinning at very high speeds, while sapphire windows do not. This renders Rayleigh interference mode data collected at high speeds using quartz windows much noisier than with sapphire windows-which we have quantified by measuring how the signal to noise ratio of Fourier transformed Rayleigh interference scans degrades at high speed. Thus, we conclude that new-generation sapphire windows can be used for all AUC experiments through almost the entire mid UV range-obviating the need for quartz windows, unless wavelengths below 220 nm must be accessed.", "gemini": "Các ô siêu ly tâm phân tích (AUC) sử dụng cửa sổ thạch anh hoặc sapphire làm nắp cuối cho vỏ ô. Không khuyến khích sử dụng cửa sổ sapphire thế hệ hiện tại để thu thập dữ liệu hấp thụ dưới 235 nm, vì vật liệu cửa sổ cho thấy sự giảm mạnh về độ truyền ở bước sóng thấp do tạp chất trong sapphire. Cửa sổ thạch anh có thể được sử dụng dưới 235 nm vì chúng không biểu hiện độ truyền bất lợi ở bước sóng thấp. Trong nghiên cứu này, chúng tôi trình bày các tính chất quang học của cửa sổ sapphire thế hệ mới và so sánh chúng với cửa sổ thạch anh trong một phạm vi rộng các bước sóng và trình bày kết quả của các thí nghiệm tốc độ lắng đọng trên BSA bằng cả hai loại cửa sổ bằng cách sử dụng dữ liệu thu thập được ở cả giá trị cực đại hấp thụ 280 nm cũng như 230-240 nm (gần với giá trị cực đại liên kết peptit). Kết quả của chúng tôi cho thấy cửa sổ thạch anh và sapphire thế hệ mới cung cấp kết quả giống hệt nhau ở chế độ hấp thụ. Chúng tôi cũng chứng minh rằng cửa sổ thạch anh bị biến dạng cơ học đáng kể khi quay ở tốc độ rất cao, trong khi cửa sổ sapphire thì không. Điều này làm cho dữ liệu chế độ giao thoa Rayleigh thu thập được ở tốc độ cao bằng cửa sổ thạch anh nhiễu hơn nhiều so với cửa sổ sapphire - điều mà chúng tôi đã định lượng bằng cách đo tỷ lệ tín hiệu trên nhiễu của các quét giao thoa Rayleigh biến đổi Fourier bị suy giảm ở tốc độ cao. Do đó, chúng tôi kết luận rằng cửa sổ sapphire thế hệ mới có thể được sử dụng cho tất cả các thí nghiệm AUC trong gần như toàn bộ phạm vi UV giữa - loại bỏ nhu cầu về cửa sổ thạch anh, trừ khi phải tiếp cận các bước sóng dưới 220 nm."} +{"idx": 1870561, "en": "PURPOSE To clarify the subjective and objective impacts of a change in a 12-h shift schedule, involving a reduction in the number of crews, on workers at an electronic parts production company. METHODS Forty-two workers participated in this study. Subjective indicators (e.g. questionnaires) and objective indicators [e.g., psychomotor vigilance task (PVT)], as well as an activity monitor-based sleep assessment, were measured during a > 1-year period that encompassed the shift schedule change. The study outcome was a comparison of work-related injury rates measured 1 year before and after the change in shift schedule. RESULTS After the shift schedule change, questionnaire scores regarding work-related burdens and PVT performance parameters, including mean response time and number of lapses, increased significantly. However, we also observed divergences in the trends of the subjective and objective measures 1 year after the change. Despite these variations in both types of measures, we observed no significant differences in work-related injury rates measured before and after the shift schedule change. CONCLUSION This study revealed that a reduction in the number of crews in a 12-h shift schedule has a negative impact on the subjective and objective indicator after the change in shift schedule; only indicators of alertness did not recover at 1 year after the change. Assessments of the workers' performances and the regular implementation of health and safety programs for more than 1 year are needed to maintain workers' health after the change in shift schedule.", "gemini": "MỤC ĐÍCH Làm rõ các tác động chủ quan và khách quan của việc thay đổi lịch làm việc theo ca 12 giờ, liên quan đến việc giảm số lượng phi hành đoàn, đối với công nhân tại một công ty sản xuất linh kiện điện tử. PHƯƠNG PHÁP Bốn mươi hai công nhân đã tham gia nghiên cứu này. Các chỉ số chủ quan (ví dụ: bảng câu hỏi) và các chỉ số khách quan [ví dụ: nhiệm vụ cảnh giác tâm vận động (PVT)], cũng như đánh giá giấc ngủ dựa trên màn hình hoạt động, đã được đo trong khoảng thời gian > 1 năm bao gồm cả thay đổi lịch làm việc theo ca. Kết quả nghiên cứu là so sánh tỷ lệ chấn thương liên quan đến công việc được đo 1 năm trước và sau khi thay đổi lịch làm việc theo ca. KẾT QUẢ Sau khi thay đổi lịch làm việc theo ca, điểm số bảng câu hỏi liên quan đến gánh nặng liên quan đến công việc và các thông số hiệu suất PVT, bao gồm thời gian phản hồi trung bình và số lần mất tập trung, đã tăng lên đáng kể. Tuy nhiên, chúng tôi cũng quan sát thấy sự khác biệt trong xu hướng của các biện pháp chủ quan và khách quan 1 năm sau khi thay đổi. Mặc dù có những thay đổi này trong cả hai loại biện pháp, chúng tôi không quan sát thấy sự khác biệt đáng kể về tỷ lệ chấn thương liên quan đến công việc được đo trước và sau khi thay đổi lịch làm việc theo ca. KẾT LUẬN Nghiên cứu này cho thấy việc giảm số lượng phi hành đoàn trong lịch làm việc theo ca 12 giờ có tác động tiêu cực đến chỉ số chủ quan và khách quan sau khi thay đổi lịch làm việc theo ca; chỉ có các chỉ số cảnh giác là không phục hồi sau 1 năm thay đổi. Cần đánh giá hiệu suất của công nhân và thực hiện thường xuyên các chương trình sức khỏe và an toàn trong hơn 1 năm để duy trì sức khỏe của công nhân sau khi thay đổi lịch làm việc theo ca."} +{"idx": 1870562, "en": "Multiple myeloma represents a subset of plasma cell dyscrasias characterized by the proliferation of plasma cells typically in the bone marrow, representing approximately 1% of all cancers and 15% of hematologic malignancies. Often multiple myeloma is limited to the skeletal system; however, a small percentage (<5%) of patients will develop extraosseous manifestations. We review the current WHO classification of plasma cell dyscrasias and use multimodality imaging including US, CT, MRI, and PET-CT to illustrate the spectrum of extraosseous multiple myeloma in the abdomen and pelvis. Because extraosseous multiple myeloma is associated with a poorer prognosis and decreased survival, it is important for the radiologist to become familiar with a variety of extraosseous manifestations in the abdomen and pelvis, especially in a patient with a known diagnosis of multiple myeloma and the development of an abdominal or pelvic mass.", "gemini": "U tủy đa đại diện cho một nhóm nhỏ các bệnh loạn sản tế bào plasma đặc trưng bởi sự tăng sinh của các tế bào plasma thường ở tủy xương, chiếm khoảng 1% trong số tất cả các bệnh ung thư và 15% trong số các bệnh ác tính về huyết học. U tủy đa thường chỉ giới hạn ở hệ thống xương; tuy nhiên, một tỷ lệ nhỏ (<5%) bệnh nhân sẽ phát triển các biểu hiện ngoài xương. Chúng tôi xem xét phân loại WHO hiện tại về các bệnh loạn sản tế bào plasma và sử dụng hình ảnh đa phương thức bao gồm siêu âm, CT, MRI và PET-CT để minh họa phổ u tủy đa ngoài xương ở bụng và vùng chậu. Do u tủy đa ngoài xương có liên quan đến tiên lượng xấu hơn và giảm tỷ lệ sống sót, nên bác sĩ chẩn đoán hình ảnh cần làm quen với nhiều biểu hiện ngoài xương ở bụng và vùng chậu, đặc biệt là ở bệnh nhân đã được chẩn đoán mắc u tủy đa và phát triển khối u ở bụng hoặc vùng chậu."} +{"idx": 1870563, "en": "PURPOSE To investigate whether pretreatment magnetic resonance (MR)-based radiomics nomogram can individualize prediction of perineural invasion (PNI) status in rectal cancer (RC). MATERIAL AND METHODS A total of 122 RC patients with pathologically confirmed were classified as training cohort (n = 87) and test cohort (n = 35). 180 radiomics features were extracted from all lesions based on oblique axial T2WI TSE images. The dimensionality reduction and feature selection in training cohort were realized by the maximum relevance minimum redundancy (mRMR) algorithm and the least absolute shrinkage and selection operator (LASSO) regression model. A predictive model combining radiomics features and clinical risk factors (pathological N stage, pathological LVI status) was established by multivariate logistic regression analysis. The performance of the model was assessed based on its receiver operating characteristic (ROC) curve, nomogram, and calibration. RESULTS The developed radiomics nomogram that integrated the radiomics signature and clinical risk factors could provide discrimination in the training and test cohorts. The accuracy and the area under the curve (AUC) for assessing PNI status were 0.82, 0.86, respectively, in the training cohort, while they were 0.71 and 0.85 in the test cohort. The goodness-of-fit of the nomogram was evaluated using the Hosmer-Lemeshow test (p = 0.52 in training cohort and p = 0.24 in test cohort). Decision curve analysis (DCA) showed that the radiomics nomogram was clinically useful. CONCLUSION The developed radiomics nomogram might be helpful in the individualized assessment PNI status in patients with RC. This stratification of RC patients according to their PNI status may provide the basis for individualized adjuvant therapy, especially for stage II patients.", "gemini": "MỤC ĐÍCH Nghiên cứu liệu chụp cộng hưởng từ (MR) dựa trên phép đo nhiễu trước khi điều trị có thể cá nhân hóa dự đoán tình trạng xâm lấn quanh dây thần kinh (PNI) trong ung thư trực tràng (RC) hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Tổng cộng 122 bệnh nhân RC được xác nhận bệnh lý được phân loại thành nhóm đào tạo (n = 87) và nhóm thử nghiệm (n = 35). 180 đặc điểm nhiễu được trích xuất từ tất cả các tổn thương dựa trên hình ảnh T2WI TSE trục nghiêng. Giảm chiều và lựa chọn đặc điểm trong nhóm đào tạo được thực hiện bằng thuật toán độ liên quan tối đa độ dư thừa tối thiểu (mRMR) và mô hình hồi quy thu nhỏ tuyệt đối tối thiểu (LASSO). Một mô hình dự đoán kết hợp các đặc điểm nhiễu và các yếu tố nguy cơ lâm sàng (giai đoạn N bệnh lý, tình trạng LVI bệnh lý) được thiết lập bằng phân tích hồi quy logistic đa biến. Hiệu suất của mô hình được đánh giá dựa trên đường cong đặc tính hoạt động của đầu thu (ROC), biểu đồ và hiệu chuẩn. KẾT QUẢ Biểu đồ nhiễu được phát triển tích hợp đặc điểm nhiễu và các yếu tố nguy cơ lâm sàng có thể phân biệt được trong nhóm đào tạo và nhóm thử nghiệm. Độ chính xác và diện tích dưới đường cong (AUC) để đánh giá tình trạng PNI lần lượt là 0,82, 0,86 trong nhóm đào tạo, trong khi ở nhóm thử nghiệm là 0,71 và 0,85. Độ phù hợp của biểu đồ được đánh giá bằng kiểm định Hosmer-Lemeshow (p = 0,52 trong nhóm đào tạo và p = 0,24 trong nhóm thử nghiệm). Phân tích đường cong quyết định (DCA) cho thấy biểu đồ nhiễu có giá trị lâm sàng. KẾT LUẬN Biểu đồ nhiễu được phát triển có thể hữu ích trong việc đánh giá tình trạng PNI cá nhân hóa ở những bệnh nhân mắc RC. Sự phân tầng này của bệnh nhân RC theo tình trạng PNI của họ có thể cung cấp cơ sở cho liệu pháp bổ trợ cá nhân hóa, đặc biệt đối với bệnh nhân giai đoạn II."} +{"idx": 1870564, "en": "Sanitizing solutions against bacterial and viral pathogens are of utmost importance in general and, in particular, in these times of pandemic due to Sars-Cov2. They frequently consist of chlorine-based solutions, or in the direct input of a certain amount of chlorine in water supply systems and swimming pools. Colorimetry is one of the techniques used to measure the crucial persistence of chlorine in water, including household chlorine test kits commonly based on colorimetric indicators. Here, we show a simple and cheap colorimetric method based on 3,3',5,5'-tetramethylbenzidine (TMB), commonly used as chromogenic reagent for enzyme-linked immunosorbent assays. TMB is converted by chlorine to a colored molecule through a pH-dependent multi-step oxidation process where the chromaticity of TMB is directly proportional to chlorine content. This molecule offers several advantages over other commonly used reagents in terms of safety, sensitivity, and, peculiarly, hue modulation, giving rise to the detection of chlorine in water with a multi-color change of the indicator solution (transparent/blue/green/yellow). Moreover, through the appropriate setting of reaction conditions, such coloration is finely tunable to cover the range of chlorine concentration recommended by international health agencies for treatment of drinking water and swimming pools and to test homemade solutions prepared by dilution of household bleach during health emergency events such as during the current pandemic. Graphical abstract.", "gemini": "Các giải pháp vệ sinh chống lại các tác nhân gây bệnh do vi khuẩn và vi-rút có tầm quan trọng tối đa nói chung và đặc biệt là trong thời kỳ đại dịch do Sars-Cov2. Chúng thường bao gồm các dung dịch gốc clo hoặc trực tiếp đưa một lượng clo nhất định vào hệ thống cấp nước và bể bơi. Phương pháp đo màu là một trong những kỹ thuật được sử dụng để đo độ bền quan trọng của clo trong nước, bao gồm cả bộ dụng cụ thử clo gia dụng thường dựa trên các chỉ thị màu. Ở đây, chúng tôi trình bày một phương pháp đo màu đơn giản và rẻ tiền dựa trên 3,3 ', 5,5'-tetramethylbenzidine (TMB), thường được sử dụng làm thuốc thử sinh màu cho xét nghiệm miễn dịch liên kết với enzyme. TMB được clo chuyển đổi thành một phân tử có màu thông qua quá trình oxy hóa nhiều bước phụ thuộc vào độ pH, trong đó độ sắc của TMB tỷ lệ thuận với hàm lượng clo. Phân tử này có một số ưu điểm so với các thuốc thử thông dụng khác về độ an toàn, độ nhạy và đặc biệt là điều chế màu sắc, giúp phát hiện clo trong nước với sự thay đổi nhiều màu của dung dịch chỉ thị (trong suốt / xanh lam / xanh lục / vàng). Hơn nữa, thông qua việc thiết lập thích hợp các điều kiện phản ứng, màu sắc như vậy có thể được điều chỉnh chính xác để bao phủ phạm vi nồng độ clo do các cơ quan y tế quốc tế khuyến nghị để xử lý nước uống và bể bơi và để thử nghiệm các giải pháp tự chế được pha loãng từ thuốc tẩy gia dụng trong các sự kiện khẩn cấp về sức khỏe như trong đại dịch hiện nay. Tóm tắt đồ họa."} +{"idx": 1870565, "en": "PURPOSE Spirulina is often used as dietary supplement for its protein content and quality. However, in vivo data on protein digestibility are lacking. This study aims to determine nitrogen and amino acid digestibility in rats. A secondary objective was to test the effect of sonication prior to ingestion to break cell walls. METHODS Wistar rats were fed a single test meal containing 15N Spirulina that was either sonicated (n = 11) or not (control, n = 13). Rats were euthanized 6 h after the meal ingestion. Spirulina nitrogen digestibility was measured by assessment of 15N recovery in digestive contents. Amino acid digestibility was measured by quantification of the caecal amino acid content and their 15N enrichment. RESULTS Real fecal nitrogen digestibility was 86.0 ± 0.7%, without any differences between groups. Mean 15N amino acid caecal digestibility was 82.8 ± 1.3%, and values ranged between 77.9 ± 1.9% for serine and 89.4 ± 1.0% for methionine. No effect of sonication was observed. The most limiting AA was histidine, with a chemical score of 0.98 and a PD-CAAS of 0.84. Lysine was also limiting in a lesser extent. CONCLUSION The nitrogen and amino acid digestibility of Spirulina is relatively low, and showed no effect of prior sonication. Its amino acid composition is relatively well balanced but not enough to compensate for the poor digestibility.", "gemini": "MỤC ĐÍCH Tảo Spirulina thường được sử dụng làm thực phẩm bổ sung vì hàm lượng và chất lượng protein của nó. Tuy nhiên, dữ liệu in vivo về khả năng tiêu hóa protein vẫn còn thiếu. Nghiên cứu này nhằm xác định khả năng tiêu hóa nitơ và axit amin ở chuột. Một mục tiêu thứ cấp là kiểm tra tác dụng của siêu âm trước khi ăn vào để phá vỡ thành tế bào. PHƯƠNG PHÁP Chuột Wistar được cho ăn một bữa ăn thử nghiệm duy nhất chứa 15N Spirulina đã được siêu âm (n = 11) hoặc không (kiểm soát, n = 13). Chuột bị chết sau 6 giờ sau khi ăn. Khả năng tiêu hóa nitơ của Spirulina được đo bằng cách đánh giá sự phục hồi 15N trong chất chứa trong đường tiêu hóa. Khả năng tiêu hóa axit amin được đo bằng cách định lượng hàm lượng axit amin trong manh tràng và sự làm giàu 15N của chúng. KẾT QUẢ Khả năng tiêu hóa nitơ phân thực tế là 86,0 ± 0,7%, không có sự khác biệt giữa các nhóm. Khả năng tiêu hóa manh tràng của axit amin 15N trung bình là 82,8 ± 1,3%, và các giá trị dao động trong khoảng 77,9 ± 1,9% đối với serine và 89,4 ± 1,0% đối với methionine. Không thấy tác dụng của siêu âm. AA hạn chế nhất là histidine, với điểm hóa học là 0,98 và PD-CAAS là 0,84. Lysine cũng hạn chế ở mức độ thấp hơn. KẾT LUẬN Khả năng tiêu hóa nitơ và axit amin của Spirulina tương đối thấp và không cho thấy tác dụng của siêu âm trước đó. Thành phần axit amin của nó tương đối cân bằng nhưng không đủ để bù đắp cho khả năng tiêu hóa kém."} +{"idx": 1870566, "en": "PURPOSE Given the disputable link between nerve growth factor (NGF) and overactive bladder syndrome (OAB) and the lack of studies on its precursor (proNGF) in OAB, the aim of the study was to identify changes in the urinary levels of NGF and its proteolytic enzymes in aging women with OAB. METHODS We examined the urinary proNGF/NGF ratio and its processing enzymes in aging women (50-80 years), comparing 20 controls and 20 subjects with OAB. RESULTS In contrast to previous reports correlating NGF to OAB symptoms, we found that proNGF/NGF ratio in the OAB group was twice as high compared to controls (p = 0.009) with a lower NGF levels in women with OAB without statistical significance [1.36 (Q1, Q3: 0.668, 2.39) vs. 1.7 (Q1, Q3: 1.27, 3.045) pg/mg creatinine in control group, p = 0.05]. Enzymatic activity of MMP-7, the main enzyme for extracellular proNGF maturation, was significantly increased in the OAB group and correlated positively with scores of OAB symptoms questionnaires. However, this was counteracted by several-folds increase in the MMP-9 enzyme responsible for NGF proteolysis. While these findings highlight the importance of changes in the proteolytic enzymes to maintain proNGF/NGF balance in OAB, analysis of covariates showed that these changes were attributed to age, insulin resistance and renal function. CONCLUSION NGF proteolysis imbalance can be clinically meaningful in OAB related to aging, rendering it as a potential therapeutic target. However, other age-related factors such as insulin resistance and renal function may contribute to the relationship between NGF and aging-related OAB phenotype.", "gemini": "MỤC ĐÍCH Do mối liên hệ gây tranh cãi giữa yếu tố tăng trưởng thần kinh (NGF) và hội chứng bàng quang hoạt động quá mức (OAB) và thiếu các nghiên cứu về tiền chất của nó (proNGF) trong OAB, mục đích của nghiên cứu là xác định những thay đổi trong nồng độ NGF trong nước tiểu và các enzym phân giải protein của nó ở phụ nữ lớn tuổi mắc OAB. PHƯƠNG PHÁP Chúng tôi đã kiểm tra tỷ lệ proNGF / NGF trong nước tiểu và các enzym xử lý của nó ở phụ nữ lớn tuổi (50-80 tuổi), so sánh 20 đối chứng và 20 đối tượng mắc OAB. KẾT QUẢ Trái ngược với các báo cáo trước đây liên quan NGF với các triệu chứng OAB, chúng tôi thấy rằng tỷ lệ proNGF / NGF ở nhóm OAB cao gấp đôi so với nhóm đối chứng (p = 0,009) với mức NGF thấp hơn ở phụ nữ mắc OAB nhưng không có ý nghĩa thống kê [1,36 (Q1, Q3: 0,668, 2,39) so với 1,7 (Q1, Q3: 1,27, 3,045) pg / mg creatinine ở nhóm đối chứng, p = 0,05]. Hoạt động của enzym MMP-7, enzym chính cho quá trình trưởng thành proNGF ngoại bào, tăng đáng kể ở nhóm OAB và tương quan thuận với điểm số của bảng câu hỏi về các triệu chứng OAB. Tuy nhiên, điều này đã được chống lại bởi sự gia tăng gấp nhiều lần của enzym MMP-9 chịu trách nhiệm cho quá trình phân giải NGF. Mặc dù những phát hiện này làm nổi bật tầm quan trọng của những thay đổi trong các enzym phân giải protein để duy trì sự cân bằng proNGF / NGF trong OAB, nhưng phân tích các biến số đồng hành cho thấy những thay đổi này là do tuổi tác, tình trạng kháng insulin và chức năng thận. KẾT LUẬN Sự mất cân bằng trong quá trình phân giải NGF có thể có ý nghĩa lâm sàng ở OAB liên quan đến tuổi tác, khiến nó trở thành mục tiêu điều trị tiềm năng. Tuy nhiên, các yếu tố liên quan đến tuổi tác khác như tình trạng kháng insulin và chức năng thận có thể góp phần vào mối quan hệ giữa NGF và kiểu hình OAB liên quan đến tuổi tác."} +{"idx": 1870567, "en": "BACKGROUND With the development of an artificial environment to support the extremely premature infant, advanced imaging techniques tested in this extrauterine system might be beneficial to evaluate the fetal brain. OBJECTIVE We evaluated the feasibility of (a) performing contrast-enhanced ultrasound (CEUS) and (b) quantifying normal and decreased brain perfusion in fetal lambs maintained on the extrauterine environment for neonatal development (EXTEND) system. MATERIALS AND METHODS Twin premature fetal lambs (102 days of gestational age) were transferred to the EXTEND system. Twin B was subjected to sub-physiological flows (152 mL/kg/min) and oxygen delivery (15.9 mL/kg/min), while Twin A was maintained at physiological levels. We administered Lumason contrast agent into the oxygenator circuit and performed serial CEUS examinations. We quantified perfusion parameters and generated parametric maps. We also recorded hemodynamic parameters, serum blood analysis, and measurements across the oxygenator. Postmortem MRIs were performed. RESULTS No significant changes in hemodynamic variables were attributable to CEUS examinations. On gray-scale images, Twin B demonstrated ventriculomegaly and progressive parenchymal volume loss culminating in hydranencephaly. By CEUS, Twin B demonstrated decreased peak enhancement and decreased overall parenchymal perfusion when compared to Twin A by perfusion parameters and parametric maps. Changes in perfusion parameters were detected immediately following blood transfusion. Postmortem MRI confirmed ultrasonographic findings in Twin B. CONCLUSION In this preliminary experience, we show that CEUS of the brain is feasible in fetal lambs maintained on the EXTEND system and that changes in perfusion can be quantified, which is promising for the application of CEUS in this extrauterine system supporting the premature infant.", "gemini": "BỐI CẢNH Với sự phát triển của một môi trường nhân tạo để hỗ trợ trẻ sơ sinh cực non, các kỹ thuật chụp ảnh tiên tiến được thử nghiệm trong hệ thống ngoài tử cung này có thể có lợi trong việc đánh giá não của thai nhi. MỤC TIÊU Chúng tôi đã đánh giá tính khả thi của (a) thực hiện siêu âm có độ tương phản (CEUS) và (b) định lượng tưới máu não bình thường và giảm ở cừu thai được nuôi trong hệ thống môi trường ngoài tử cung để phát triển sơ sinh (EXTEND). VẬT LIỆU VÀ PHƯƠNG PHÁP Cặp cừu thai non sinh đôi (thai 102 ngày tuổi) được chuyển sang hệ thống EXTEND. Cừu thai B được đưa vào dòng chảy dưới sinh lý (152 mL/kg/phút) và cung cấp oxy (15,9 mL/kg/phút), trong khi Cừu thai A được duy trì ở mức sinh lý. Chúng tôi đưa chất cản quang Lumason vào mạch ôxy hóa và thực hiện các lần kiểm tra CEUS theo chuỗi. Chúng tôi định lượng các thông số tưới máu và tạo ra các bản đồ tham số. Chúng tôi cũng ghi lại các thông số huyết động, phân tích máu huyết thanh và các phép đo trên máy ôxy hóa. Chụp MRI sau tử thi được thực hiện. KẾT QUẢ Không có thay đổi đáng kể nào về các biến số huyết động học là do các lần kiểm tra CEUS. Trên hình ảnh thang độ xám, Cừu thai B cho thấy não úng thủy và mất thể tích nhu mô tiến triển dẫn đến não úng thủy. Bằng CEUS, Cừu thai B cho thấy độ tăng cường đỉnh giảm và tưới máu nhu mô tổng thể giảm khi so sánh với Cừu thai A theo các thông số tưới máu và bản đồ tham số. Những thay đổi về các thông số tưới máu được phát hiện ngay sau khi truyền máu. MRI sau tử thi đã xác nhận các phát hiện siêu âm ở Cừu thai B. KẾT LUẬN Trong kinh nghiệm sơ bộ này, chúng tôi cho thấy CEUS của não là khả thi ở cừu thai được nuôi trong hệ thống EXTEND và những thay đổi về tưới máu có thể được định lượng, điều này hứa hẹn cho ứng dụng CEUS trong hệ thống ngoài tử cung này hỗ trợ trẻ sơ sinh non tháng."} +{"idx": 1870568, "en": "BACKGROUND Very preterm birth can disturb brain maturation and subject these high-risk children to neurocognitive difficulties later. OBJECTIVE The aim of the study was to evaluate the impact of prematurity on microstructure of frontostriatal tracts in children with no severe neurologic impairment, and to study whether the diffusion tensor imaging metrics of frontostriatal tracts correlate to executive functioning. MATERIALS AND METHODS The prospective cohort study comprised 54 very preterm children (mean gestational age 28.8 weeks) and 20 age- and gender-matched term children. None of the children had severe neurologic impairment. The children underwent diffusion tensor imaging and neuropsychological assessments at a mean age of 9 years. We measured quantitative diffusion tensor imaging metrics of frontostriatal tracts using probabilistic tractography. We also administered five subtests from the Developmental Neuropsychological Assessment, Second Edition, to evaluate executive functioning. RESULTS Very preterm children had significantly higher fractional anisotropy and axial diffusivity values (P<0.05, corrected for multiple comparison) in dorsolateral prefrontal caudate and ventrolateral prefrontal caudate tracts as compared to term-born children. We found negative correlations between the diffusion tensor imaging metrics of frontostriatal tracts and inhibition functions (P<0.05, corrected for multiple comparison) in very preterm children. CONCLUSION Prematurity has a long-term effect on frontostriatal white matter microstructure that might contribute to difficulties in executive functioning.", "gemini": "BỐI CẢNH Sinh non cực kỳ có thể làm rối loạn quá trình trưởng thành của não và khiến những trẻ em có nguy cơ cao này gặp khó khăn về nhận thức sau này. MỤC TIÊU Mục đích của nghiên cứu này là đánh giá tác động của sinh non đối với vi cấu trúc của đường dẫn trán-vân trong trẻ em không bị suy giảm thần kinh nghiêm trọng và nghiên cứu xem các số liệu chụp ảnh độ khuếch tán tensor của đường dẫn trán-vân có tương quan với chức năng điều hành hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu theo dõi triển vọng bao gồm 54 trẻ sinh non cực kỳ (tuổi thai trung bình 28,8 tuần) và 20 trẻ đủ tháng được ghép đôi theo tuổi và giới tính. Không có trẻ nào bị suy giảm thần kinh nghiêm trọng. Trẻ em được chụp ảnh độ khuếch tán tensor và đánh giá tâm lý thần kinh ở độ tuổi trung bình là 9. Chúng tôi đã đo các số liệu chụp ảnh độ khuếch tán tensor định lượng của đường dẫn trán-vân bằng phương pháp theo dõi xác suất. Chúng tôi cũng tiến hành năm bài kiểm tra phụ từ Đánh giá tâm lý thần kinh phát triển, Phiên bản thứ hai để đánh giá chức năng điều hành. KẾT QUẢ Trẻ sinh non cực kỳ có giá trị dị hướng phân số và độ khuếch tán trục cao hơn đáng kể (P <0,05, hiệu chỉnh cho nhiều so sánh) ở đường dẫn trán lưng bên nhân đuôi và đường dẫn trán trước bên bụng so với trẻ sinh đủ tháng. Chúng tôi tìm thấy mối tương quan âm giữa các số liệu chụp ảnh độ khuếch tán tensor của đường dẫn trán-vân và chức năng ức chế (P <0,05, hiệu chỉnh cho nhiều so sánh) ở trẻ sinh non cực kỳ. KẾT LUẬN Sinh non có tác động lâu dài đến vi cấu trúc chất trắng trán-vân, có thể góp phần gây khó khăn trong chức năng điều hành."} +{"idx": 1870569, "en": "Budd-Chiari syndrome is a vascular pathology of the liver, commonly seen in adults and uncommon in children. Most children with Budd-Chiari syndrome present with ascites, and an etiology is found in only about 50%. Ultrasonography (US) with color Doppler is the main modality used in the diagnosis. US imaging additionally guides radiologic interventions and follow-up after recanalization or shunt procedure. In this pictorial review, we illustrate the findings in pediatric Budd-Chiari syndrome as seen on B-mode and color Doppler US and describe the role of US in guiding percutaneous radiologic interventions, with a brief description of the role of US contrast agent and sonoelastography in this setting.", "gemini": "Hội chứng Budd-Chiari là một bệnh lý mạch máu của gan, thường gặp ở người lớn và không phổ biến ở trẻ em. Hầu hết trẻ em mắc hội chứng Budd-Chiari đều biểu hiện cổ trướng và chỉ tìm thấy nguyên nhân ở khoảng 50%. Siêu âm (US) với Doppler màu là phương thức chính được sử dụng trong chẩn đoán. Chụp US ngoài ra còn hướng dẫn các can thiệp về X quang và theo dõi sau khi tái thông hoặc thủ thuật đặt ống thông. Trong bài tổng quan bằng hình ảnh này, chúng tôi minh họa các phát hiện về hội chứng Budd-Chiari ở trẻ em khi thấy trên chế độ B và Doppler màu US và mô tả vai trò của US trong việc hướng dẫn các can thiệp X quang qua da, có mô tả ngắn gọn về vai trò của chất cản quang US và siêu âm đàn hồi trong trường hợp này."} +{"idx": 1870570, "en": "BACKGROUND Various perinatal morbidities may adversely affect postnatal nephrogenesis in preterm infants. Kidney ultrasonographic findings following acute kidney injury (AKI) have not been well described in preterm infants. Herein, we describe three cases of extremely preterm infants who showed abnormal kidney ultrasonographic findings resembling dysplasia of the kidneys following AKI. CASE-DIAGNOSIS/TREATMENT Their median gestational age and birth weight were 25+6 (range 23+3-26+6) weeks and 620 (480-840) g, respectively. All infants suffered severe AKI during their third to seventh week of life. Their kidney function recovered with conventional management. Kidney ultrasonographies performed after AKI revealed increased kidney echogenicity, loss of corticomedullary differentiation, and multiple cortical cysts, which were similar to cystic dysplasia of the kidneys and were absent in previous kidney imaging. Three infants eventually developed at least one of the long-term kidney sequelae following AKI, including proteinuria, hypertension, and elevated levels of serum creatinine or cystatin C as determined during the last follow-up at the corrected age of 9-18 months. CONCLUSIONS Based on these cases, we can infer that AKI occurring during the early postnatal period may result in dysplasia of the kidneys with cortical cysts in extremely preterm infants, which may lead to chronic kidney disease in their later life. It is useful to follow up not only laboratory parameters but also kidney ultrasonographic findings in extremely preterm infants who suffered AKI during their early postnatal periods.", "gemini": "BỐI CẢNH Nhiều bệnh lý chu sinh khác nhau có thể ảnh hưởng xấu đến quá trình sinh thận sau sinh ở trẻ sinh non. Các phát hiện siêu âm thận sau tổn thương thận cấp (AKI) vẫn chưa được mô tả rõ ở trẻ sinh non. Trong bài này, chúng tôi mô tả ba trường hợp trẻ sinh cực non có các phát hiện siêu âm thận bất thường giống như loạn sản thận sau AKI. CHẨN ĐOÁN/ĐIỀU TRỊ TRƯỜNG HỢP Tuổi thai trung bình và cân nặng khi sinh của trẻ lần lượt là 25+6 (phạm vi 23+3-26+6) tuần và 620 (480-840) g. Tất cả trẻ sơ sinh đều bị AKI nghiêm trọng trong tuần thứ ba đến tuần thứ bảy của cuộc đời. Chức năng thận của trẻ phục hồi với cách xử trí thông thường. Siêu âm thận thực hiện sau AKI cho thấy tăng độ hồi âm của thận, mất sự phân biệt vỏ tủy và nhiều nang ở vỏ, tương tự như loạn sản nang thận và không có trong hình ảnh thận trước đó. Cuối cùng, ba trẻ sơ sinh đã phát triển ít nhất một trong những di chứng thận dài hạn sau AKI, bao gồm protein niệu, tăng huyết áp và nồng độ creatinine huyết thanh hoặc cystatin C tăng như xác định trong lần theo dõi cuối cùng khi trẻ được hiệu chỉnh ở độ tuổi 9-18 tháng. KẾT LUẬN Dựa trên những trường hợp này, chúng tôi có thể suy ra rằng AKI xảy ra trong giai đoạn đầu sau sinh có thể dẫn đến loạn sản thận với các nang ở vỏ ở trẻ sinh cực non, có thể dẫn đến bệnh thận mạn tính ở giai đoạn sau của cuộc đời. Việc theo dõi không chỉ các thông số xét nghiệm mà còn cả các phát hiện siêu âm thận ở trẻ sinh cực non bị AKI trong giai đoạn đầu sau sinh là rất hữu ích."} +{"idx": 1870571, "en": "BACKGROUND The five Nordic countries with a population of 27M people form a rather homogenous region in terms of health care. The management of Head and Neck Cancer (HNC) is centralized to the 21 university hospitals in these countries. Our aim was to survey the current status of organization of palliative care for patients with HNC in the Nordic countries as the field is rapidly developing. MATERIALS AND METHODS A structured web-based questionnaire was sent to all the Departments of Otorhinolaryngology-Head and Neck Surgery and Oncology managing HNC in the Nordic countries. RESULTS All 21 (100%) Nordic university hospitals responded to the survey. A majority (over 90%) of the patients are discussed at diagnosis in a multidisciplinary tumor board (MDT), but the presence of a palliative care specialist is lacking in 95% of these MDT's. The patients have access to specialized palliative care units (n = 14, 67%), teams (n = 10, 48%), and consultants (n = 4, 19%) in the majority of the hospitals. CONCLUSION The present results show that specialized palliative care services are available at the Nordic university hospitals. A major finding was that the collaboration between head and neck surgeons, oncologists and palliative care specialists is not well structured and the palliative care pathway of patients with HNC is not systematically organized. We suggest that early integrated palliative care needs to be included as an addition to the already existing HNC care pathways in the Nordic countries.", "gemini": "BỐI CẢNH Năm quốc gia Bắc Âu với dân số 27 triệu người tạo thành một khu vực khá đồng nhất về mặt chăm sóc sức khỏe. Việc quản lý Ung thư đầu cổ (HNC) được tập trung tại 21 bệnh viện đại học ở các quốc gia này. Mục đích của chúng tôi là khảo sát tình trạng hiện tại của việc tổ chức chăm sóc giảm nhẹ cho bệnh nhân HNC ở các quốc gia Bắc Âu vì lĩnh vực này đang phát triển nhanh chóng. VẬT LIỆU VÀ PHƯƠNG PHÁP Một bảng câu hỏi có cấu trúc trên web đã được gửi đến tất cả các Khoa Tai mũi họng - Phẫu thuật đầu cổ và Ung bướu quản lý HNC ở các quốc gia Bắc Âu. KẾT QUẢ Cả 21 (100%) bệnh viện đại học Bắc Âu đã trả lời khảo sát. Phần lớn (trên 90%) bệnh nhân được thảo luận khi chẩn đoán tại hội đồng khối u đa chuyên khoa (MDT), nhưng 95% các MDT này không có chuyên gia chăm sóc giảm nhẹ. Bệnh nhân có thể tiếp cận các đơn vị chăm sóc giảm nhẹ chuyên khoa (n = 14, 67%), nhóm (n = 10, 48%) và bác sĩ tư vấn (n = 4, 19%) tại hầu hết các bệnh viện. KẾT LUẬN Các kết quả hiện tại cho thấy các dịch vụ chăm sóc giảm nhẹ chuyên khoa có sẵn tại các bệnh viện đại học Bắc Âu. Một phát hiện chính là sự hợp tác giữa các bác sĩ phẫu thuật đầu cổ, bác sĩ ung bướu và bác sĩ chăm sóc giảm nhẹ không được cấu trúc tốt và lộ trình chăm sóc giảm nhẹ của bệnh nhân HNC không được tổ chức một cách có hệ thống. Chúng tôi đề xuất rằng cần đưa chăm sóc giảm nhẹ tích hợp sớm vào các lộ trình chăm sóc HNC đã có ở các quốc gia Bắc Âu."} +{"idx": 1870572, "en": "PURPOSE The aim of this meta-analysis is to assess the correlation between blood transfusions and the medical/surgical complications after head and neck reconstructive surgery. METHODS The PRISMA protocol was used and the literature search was performed on Pubmed, Scopus, Cochrane Library and Web of Science up to March 13, 2020. The risk of bias in individual studies was assessed through the Newcastle Ottawa Scale. RESULTS A total of 1219 records were screened after the electronic search, 22 of which were included in the qualitative analysis. Of there 22 scores, 18 articles were included in the meta-analysis. The OR for medical and surgical complications of transfused patients was 1,64 (95% CI 1.23-2.21); while, the OR for hospital readmission was 1.53 (95% CI 1.29-1.81). CONCLUSIONS The results of this meta-analysis suggested that blood transfusions are associated with both an increased risk of surgical and medical complications of head and neck flaps and with the hospital readmission.", "gemini": "MỤC ĐÍCH Mục đích của phân tích tổng hợp này là đánh giá mối tương quan giữa truyền máu và các biến chứng y khoa/phẫu thuật sau phẫu thuật tái tạo đầu cổ. PHƯƠNG PHÁP Giao thức PRISMA đã được sử dụng và tìm kiếm tài liệu được thực hiện trên Pubmed, Scopus, Thư viện Cochrane và Web of Science cho đến ngày 13 tháng 3 năm 2020. Nguy cơ sai lệch trong các nghiên cứu riêng lẻ được đánh giá thông qua Thang điểm Newcastle Ottawa. KẾT QUẢ Tổng cộng 1219 hồ sơ đã được sàng lọc sau khi tìm kiếm điện tử, 22 trong số đó được đưa vào phân tích định tính. Trong số 22 điểm đó, 18 bài báo được đưa vào phân tích tổng hợp. OR đối với các biến chứng y khoa và phẫu thuật của bệnh nhân được truyền máu là 1,64 (95% CI 1,23-2,21); trong khi OR đối với việc nhập viện trở lại là 1,53 (95% CI 1,29-1,81). KẾT LUẬN Kết quả của phân tích tổng hợp này cho thấy truyền máu có liên quan đến cả việc tăng nguy cơ biến chứng phẫu thuật và y khoa của vạt đầu cổ và việc nhập viện trở lại."} +{"idx": 1870573, "en": "PURPOSE Post-irradiation vasculopathy is a severe form of atherosclerosis and affects the prognosis of head and neck cancer survivors. Sonographic intima-media thickness (IMT) precedes stenosis, plaque formation, and cerebrovascular events. Therefore, IMT may be a valuable screening marker for post-irradiation toxicity. However, the critical irradiation dose and the onset of IMT increase remain unclear. METHODS The cross-sectional study analysed the carotid artery IMT in 96 irradiated patients and 41 controls regarding irradiation dose, post-irradiation-interval, and cardiovascular risk factors. Distinct irradiation doses to the tumour side and the contralateral hemineck enabled detection of dose depended effects within one patient and control of risk factors. RESULTS Radiotherapy caused a dose-dependent increase in IMT. The toxicity did not have saturation effects for > 60 Gy. The IMT increase occurred in short-term following radiotherapy and the risk for a pathological value (> 0.9 mm) rose significantly. The correlation between IMT and radiotherapy was comparable to established cardiovascular risk factors. CONCLUSION Radiotherapists should consider the additional toxicity of high doses for non-metastatic head and neck cancer. If neck metastases require radiotherapy with boost, IMT measurement is suitable for early detection of carotid artery damage.", "gemini": "MỤC ĐÍCH Bệnh mạch máu sau xạ trị là một dạng xơ vữa động mạch nghiêm trọng và ảnh hưởng đến tiên lượng của những người sống sót sau ung thư đầu và cổ. Độ dày lớp trung mô-lòng mạch (IMT) siêu âm đi trước tình trạng hẹp, hình thành mảng bám và các biến cố mạch máu não. Do đó, IMT có thể là một dấu hiệu sàng lọc có giá trị đối với độc tính sau xạ trị. Tuy nhiên, liều xạ quan trọng và thời điểm bắt đầu tăng IMT vẫn chưa rõ ràng. PHƯƠNG PHÁP Nghiên cứu cắt ngang đã phân tích IMT động mạch cảnh ở 96 bệnh nhân đã được xạ trị và 41 bệnh nhân đối chứng liên quan đến liều xạ trị, khoảng thời gian sau xạ trị và các yếu tố nguy cơ tim mạch. Liều xạ khác nhau rõ rệt ở bên có khối u và bên cổ đối diện cho phép phát hiện các tác động phụ thuộc vào liều trong một bệnh nhân và kiểm soát các yếu tố nguy cơ. KẾT QUẢ Xạ trị gây ra sự gia tăng IMT phụ thuộc vào liều. Độc tính không có tác dụng bão hòa đối với liều > 60 Gy. Sự gia tăng IMT xảy ra trong thời gian ngắn sau xạ trị và nguy cơ giá trị bệnh lý (> 0,9 mm) tăng lên đáng kể. Mối tương quan giữa IMT và xạ trị tương đương với các yếu tố nguy cơ tim mạch đã được xác định. KẾT LUẬN Các bác sĩ xạ trị nên xem xét độc tính bổ sung của liều cao đối với ung thư đầu và cổ không di căn. Nếu di căn cổ cần xạ trị với liều tăng cường, thì việc đo IMT phù hợp để phát hiện sớm tổn thương động mạch cảnh."} +{"idx": 1870574, "en": "Acute myeloid leukemia (AML) patients undergoing consolidation chemotherapy with intermediate or high-dose cytarabine (IDAC/HiDAC) are often placed on prophylactic antibacterials. This practice is largely based on the benefits of prophylaxis (PPX) during induction chemotherapy. However, recent concerns regarding antibacterial prophylaxis have emerged including risk of Clostridioides difficile colitis, medication toxicities, and the potential for fostering multidrug-resistant pathogens. We therefore retrospectively explored whether antibacterial PPX is beneficial during cytarabine consolidation. Adult AML patients who received IDAC/HiDAC at our institution from January 2007 to March 2018 were evaluated for receipt of antibacterial PPX. The primary endpoint was rate of febrile neutropenia (FN); secondary endpoints were rates of unplanned hospitalization, bacteremia, infection from resistant organisms, C. difficile colitis, and death from infection. One hundred twenty patients with data from 229 IDAC/HiDAC cycles were included. Patients who received antibacterial PPX were more often hospitalized during cytarabine cycle 1 (C1) than those who received no PPX. Patients who received PPX had significantly more episodes of bacteremia, in addition to infections from resistant, predominantly Gram-positive organisms during cycle 1 of consolidation than those without PPX. Antibacterial PPX during IDAC/HiDAC consolidation treatment at our institution did not decrease the rates of FN, hospitalization, or bacteremia and was associated with higher risk of infection from drug-resistant bacteria in C1. Prospective studies examining antibacterial prophylaxis during cytarabine consolidation for AML patients are necessary, with strong consideration made for institution-specific protocols.", "gemini": "Những bệnh nhân mắc bệnh bạch cầu myeloid cấp tính (AML) đang trải qua hóa trị củng cố với cytarabine liều trung bình hoặc cao (IDAC/HiDAC) thường được dùng thuốc kháng khuẩn dự phòng. Thực hành này phần lớn dựa trên lợi ích của dự phòng (PPX) trong quá trình hóa trị gây bệnh. Tuy nhiên, những lo ngại gần đây liên quan đến dự phòng kháng khuẩn đã xuất hiện bao gồm nguy cơ viêm đại tràng do Clostridioides difficile, độc tính của thuốc và khả năng thúc đẩy các mầm bệnh đa kháng thuốc. Do đó, chúng tôi đã nghiên cứu hồi cứu xem liệu PPX kháng khuẩn có mang lại lợi ích trong quá trình củng cố cytarabine hay không. Những bệnh nhân AML trưởng thành được điều trị bằng IDAC/HiDAC tại cơ sở của chúng tôi từ tháng 1 năm 2007 đến tháng 3 năm 2018 đã được đánh giá để nhận PPX kháng khuẩn. Điểm cuối chính là tỷ lệ giảm bạch cầu trung tính do sốt (FN); các điểm cuối thứ cấp là tỷ lệ nhập viện không theo kế hoạch, nhiễm khuẩn huyết, nhiễm trùng do vi khuẩn kháng thuốc, viêm đại tràng do C. difficile và tử vong do nhiễm trùng. Một trăm hai mươi bệnh nhân có dữ liệu từ 229 chu kỳ IDAC/HiDAC đã được đưa vào. Những bệnh nhân được dùng PPX kháng khuẩn thường nhập viện nhiều hơn trong chu kỳ cytarabine 1 (C1) so với những bệnh nhân không dùng PPX. Những bệnh nhân dùng PPX có nhiều đợt nhiễm khuẩn huyết hơn đáng kể, ngoài ra còn có các trường hợp nhiễm trùng do vi khuẩn kháng thuốc, chủ yếu là vi khuẩn Gram dương trong chu kỳ củng cố thứ 1 so với những bệnh nhân không dùng PPX. PPX kháng khuẩn trong quá trình điều trị củng cố IDAC/HiDAC tại cơ sở của chúng tôi không làm giảm tỷ lệ FN, nhập viện hoặc nhiễm khuẩn huyết và có liên quan đến nguy cơ nhiễm trùng do vi khuẩn kháng thuốc cao hơn ở C1. Các nghiên cứu triển vọng kiểm tra dự phòng kháng khuẩn trong quá trình củng cố cytarabine cho bệnh nhân AML là cần thiết, với sự cân nhắc kỹ lưỡng đối với các giao thức cụ thể của cơ sở."} +{"idx": 1870575, "en": "Patients with beta thalassemia are benefitting from longer life expectancies, highlighting the importance of appropriate transition from pediatric to adult care. Data are limited regarding continuity of care and adult hematologists' management of patients with beta thalassemia. We conducted a survey of practicing US hematologists to identify practice gaps, attitudes, and barriers to optimal patient management among US-practicing hematologists. A total of 42 responses were collected, with 19 (45%) practicing at a beta thalassemia center of excellence (CoE). Nearly 90% of CoE physicians said they had a transition protocol or plan in place versus 30% of non-CoE physicians. Most physicians said parents should remain actively involved in medical visits. Adherence was rated as the most important patient education topic during transition. The most significant barrier cited was patient reluctance to transition away from pediatric care. Physicians in CoEs as compared with non-CoE physicians reported greater knowledge of beta thalassemia and familiarity with butyrates, gene therapy, and luspatercept. Highly rated topics for beta thalassemia-focused CME activities included management of complications and clinical trial updates. These findings suggest practice gaps and barriers to optimal care in the transition from pediatric to adult care, the ongoing management of adult patients, knowledge of the disease state, and familiarity with emerging treatments. Differences CoE vs non-CoE physician responses suggest variations in knowledge, practice, and attitudes that may be helpful in tailoring CME activities to different learner audiences. The small sample size used in some sub-analyses may not be representative of all hematologists treating beta thalassemia patients.", "gemini": "Những bệnh nhân mắc bệnh tan máu bẩm sinh beta đang được hưởng lợi từ tuổi thọ cao hơn, làm nổi bật tầm quan trọng của việc chuyển đổi phù hợp từ chăm sóc nhi khoa sang chăm sóc người lớn. Dữ liệu còn hạn chế liên quan đến tính liên tục của việc chăm sóc và cách quản lý bệnh nhân mắc bệnh tan máu bẩm sinh beta của các bác sĩ huyết học người lớn. Chúng tôi đã tiến hành khảo sát các bác sĩ huyết học đang hành nghề tại Hoa Kỳ để xác định những khoảng trống trong thực hành, thái độ và rào cản đối với việc quản lý bệnh nhân tối ưu trong số các bác sĩ huyết học đang hành nghề tại Hoa Kỳ. Tổng cộng có 42 phản hồi được thu thập, với 19 (45%) hành nghề tại một trung tâm xuất sắc về bệnh tan máu bẩm sinh beta (CoE). Gần 90% bác sĩ tại CoE cho biết họ có giao thức hoặc kế hoạch chuyển đổi tại chỗ so với 30% bác sĩ không làm việc tại CoE. Hầu hết các bác sĩ cho biết cha mẹ nên tiếp tục tham gia tích cực vào các buổi khám bệnh. Sự tuân thủ được đánh giá là chủ đề giáo dục bệnh nhân quan trọng nhất trong quá trình chuyển đổi. Rào cản đáng kể nhất được trích dẫn là bệnh nhân miễn cưỡng chuyển đổi khỏi dịch vụ chăm sóc nhi khoa. Các bác sĩ tại CoE so với các bác sĩ không làm việc tại CoE báo cáo có kiến thức sâu rộng hơn về bệnh tan máu bẩm sinh beta và quen thuộc với butyrat, liệu pháp gen và luspatercept. Các chủ đề được đánh giá cao cho các hoạt động CME tập trung vào bệnh tan máu bẩm sinh beta bao gồm quản lý các biến chứng và cập nhật thử nghiệm lâm sàng. Những phát hiện này cho thấy những khoảng trống trong thực hành và rào cản đối với việc chăm sóc tối ưu trong quá trình chuyển đổi từ chăm sóc nhi khoa sang chăm sóc người lớn, việc quản lý liên tục bệnh nhân người lớn, kiến thức về tình trạng bệnh và sự quen thuộc với các phương pháp điều trị mới nổi. Sự khác biệt giữa phản hồi của bác sĩ tại CoE so với bác sĩ không làm việc tại CoE cho thấy sự khác biệt về kiến thức, thực hành và thái độ có thể hữu ích trong việc điều chỉnh các hoạt động CME cho các đối tượng học viên khác nhau. Kích thước mẫu nhỏ được sử dụng trong một số phân tích phụ có thể không đại diện cho tất cả các bác sĩ huyết học điều trị bệnh nhân mắc bệnh tan máu bẩm sinh beta."} +{"idx": 1870576, "en": "Amine group-containing isoreticular metal-organic framework (IRMOF-3) particles are utilized for the first time as a trinitrotoluene (TNT) sensing material. IRMOF-3 particles are synthesized using zinc nitrate as a metal precursor and 2-amino-1,4-benzenedicarboxylic acid as a linker. The nitrogen-doped carbon quantum dots (NCQDs) are synthesized from citric acid and ethylenediamine as carbon and nitrogen precursor, respectively. The NCQDs are conjugated with IRMOF-3 particles as IRMOF-3/NCQDs. The TEM micrograph revealed the average size of IRMOF-3 particles to be 363.66 nm. The photoluminescence emission intensity of IRMOF-3 particles at λem 430 nm is highly increased in the presence of NCQDs (λex 330 nm). Both the as-synthesized IRMOF-3 and IRMOF-3/NCQD particles are explored for TNT detection to compare the effect of NCQDs on the IRMOF-3 particle surface. Lower limit of detection (7.5 × 10-8 M) and higher Stern-Volmer constant (4.46 × 106 M-1) are achieved by IRMOF-3/NCQD particles. The association constant also increased from 5.3 × 104 to 2.78 × 106 M-1 after the conjugation of IRMOF-3 particles with NCQDs. Moreover, enhanced selectivity for TNT over trinitrophenol is achieved using the IRMOF-3/NCQD particles. Graphical Abstract.", "gemini": "Các hạt khung kim loại-hữu cơ isoreticular chứa nhóm amin (IRMOF-3) được sử dụng lần đầu tiên làm vật liệu cảm biến trinitrotoluen (TNT). Các hạt IRMOF-3 được tổng hợp bằng cách sử dụng kẽm nitrat làm tiền chất kim loại và axit 2-amino-1,4-benzenedicarboxylic làm chất liên kết. Các chấm lượng tử carbon có nitơ (NCQD) được tổng hợp từ axit citric và ethylenediamin làm tiền chất carbon và nitơ, tương ứng. Các NCQD được liên hợp với các hạt IRMOF-3 dưới dạng IRMOF-3/NCQD. Ảnh chụp TEM cho thấy kích thước trung bình của các hạt IRMOF-3 là 363,66 nm. Cường độ phát quang của các hạt IRMOF-3 ở λem 430 nm tăng cao khi có NCQD (λex 330 nm). Cả các hạt IRMOF-3 được tổng hợp và hạt IRMOF-3/NCQD đều được khám phá để phát hiện TNT nhằm so sánh tác dụng của NCQD trên bề mặt hạt IRMOF-3. Hạt IRMOF-3/NCQD đạt được giới hạn phát hiện thấp hơn (7,5 × 10-8 M) và hằng số Stern-Volmer cao hơn (4,46 × 106 M-1). Hằng số liên kết cũng tăng từ 5,3 × 104 lên 2,78 × 106 M-1 sau khi liên hợp các hạt IRMOF-3 với NCQD. Hơn nữa, tính chọn lọc tăng cường đối với TNT so với trinitrophenol đạt được khi sử dụng các hạt IRMOF-3/NCQD. Trừu tượng đồ họa."} +{"idx": 1870577, "en": "Visual similarity effects are pervasive in masked priming (e.g., T4BLE→TABLE; obiect→OBJECT; docurnent→DOCUMENT) and can be easily explained in terms of uncertainty regarding letter identity. However, recent research failed to show visual similarity effects for primes containing accented vowels (e.g., féliz-FELIZ behaves as fáliz-FELIZ [happy in Spanish]). This null effect has been taken to suggest that accented and non-accented vowels (e.g., é and e) activate completely distinct representations. However, priming effects are reinstated for non-accented vowels (e.g., facil-FÁCIL < fecil-FÁCIL [easy in Spanish]). Here we tested the hypothesis that the lack of priming effects for primes containing accented vowels is a simple consequence of the saliency of the accent marks. To investigate this issue, we conducted a masked priming lexical decision experiment in which we minimized the saliency of the diacritical marks by using primes containing the letter i (i.e., a letter that contains itself a glyph over the letter). We manipulated prime-target visual similarity and the presence/absence of an accented vowel in the prime (e.g., obieto-OBJETO vs. obaeto-OBJETO; obíeto-OBJETO vs. obáeto-OBJETO [object in Spanish]). Results showed a sizeable visual similarity effect regardless of whether the prime was accented or not. Therefore, these findings suggest that, at least in scripts like Spanish, there is nothing special about the processing of accented vs. unaccented vowels once the saliency of the diacritical marks is reduced.", "gemini": "Các hiệu ứng tương đồng trực quan rất phổ biến trong việc chuẩn bị ngụy trang (ví dụ: T4BLE→TABLE; obiect→OBJECT; docurnent→DOCUMENT) và có thể dễ dàng giải thích theo sự không chắc chắn về danh tính của chữ cái. Tuy nhiên, nghiên cứu gần đây không chỉ ra hiệu ứng tương đồng trực quan đối với các nguyên tố có chứa nguyên âm có dấu (ví dụ: féliz-FELIZ hoạt động như fáliz-FELIZ [có nghĩa là vui vẻ trong tiếng Tây Ban Nha]). Hiệu ứng rỗng này được cho là gợi ý rằng nguyên âm có dấu và không dấu (ví dụ: é và e) kích hoạt các biểu diễn hoàn toàn khác biệt. Tuy nhiên, các hiệu ứng chuẩn bị được khôi phục đối với các nguyên âm không dấu (ví dụ: facil-FÁCIL < fecil-FÁCIL [có nghĩa là dễ dàng trong tiếng Tây Ban Nha]). Ở đây, chúng tôi đã kiểm tra giả thuyết rằng việc thiếu hiệu ứng chuẩn bị đối với các nguyên tố có chứa nguyên âm có dấu chỉ là một hệ quả đơn giản của sự nổi bật của các dấu trọng âm. Để nghiên cứu vấn đề này, chúng tôi đã tiến hành một thí nghiệm quyết định từ vựng chuẩn bị ngụy trang, trong đó chúng tôi giảm thiểu sự nổi bật của các dấu phụ bằng cách sử dụng các nguyên tố có chứa chữ cái i (tức là một chữ cái chứa chính nó một ký tự trên chữ cái). Chúng tôi thao tác sự tương đồng trực quan nguyên tố-mục tiêu và sự có/không có nguyên âm có dấu trong nguyên tố (ví dụ: obieto-OBJETO so với obaeto-OBJETO; obíeto-OBJETO so với obáeto-OBJETO [có nghĩa là vật thể trong tiếng Tây Ban Nha]). Kết quả cho thấy một hiệu ứng tương đồng trực quan đáng kể bất kể nguyên tố có dấu hay không. Do đó, những phát hiện này cho thấy rằng, ít nhất là trong các kịch bản như tiếng Tây Ban Nha, không có gì đặc biệt về quá trình xử lý nguyên âm có dấu so với nguyên âm không dấu sau khi giảm độ nổi bật của các dấu phụ."} +{"idx": 1870578, "en": "PURPOSE To investigate anxiety and depression levels in prosthetic eye-wearing patients using standardized psychometric instruments, to define factors associated with these psychological diseases, and to identify a potential healthcare gap. METHODS A total of 295 prosthetic eye wearers were screened using the 7-item generalized anxiety disorder scale (GAD-7) and the 9-item patient health questionnaire (PHQ-9). Scores of GAD-7 and PHQ-9 were correlated with scores of general physical and mental health functioning, vision-related quality of life, appearance-related distress, appearance-related social function, and further biosocial factors. RESULTS Five patients (2%) had a pre-diagnosed anxiety disorder, and 20 patients (7%) had a pre-diagnosed depression. However, our screening revealed 26 patients (9%) with anxiety symptoms, 31 patients (11%) with depression symptoms, and 40 patients (14%) suffering from both anxiety and depression symptoms. This underdiagnosing for both anxiety and depression disorders was significant (p < 0.001, respectively). Higher GAD-7 scores were significantly associated with higher PHQ-9 scores, lower appearance-related social function, lower mental health functioning, and female gender (p ≤ 0.021, respectively). Higher PHQ-9 scores were significantly associated with lower physical and mental health functioning, higher educational degree, and non-traumatic eye loss (p ≤ 0.038, respectively). CONCLUSIONS Anxiety and depression disorders seem to be underdiagnosed in prosthetic eye wearers and to have higher incidence compared with the general population. Therefore, a psychometric screening should be routinely implemented in the clinical care. For a successful long-term rehabilitation, integrated care by a multidisciplinary team including ophthalmic plastic surgeons, ophthalmologists, ocularists, general practitioners, and psychologists is essential.", "gemini": "MỤC ĐÍCH Nghiên cứu mức độ lo âu và trầm cảm ở những bệnh nhân đeo mắt giả bằng các công cụ trắc nghiệm tâm lý chuẩn hóa, xác định các yếu tố liên quan đến các bệnh lý tâm lý này và xác định khoảng cách chăm sóc sức khỏe tiềm ẩn. PHƯƠNG PHÁP Tổng cộng 295 người đeo mắt giả đã được sàng lọc bằng thang đo rối loạn lo âu toàn thể 7 mục (GAD-7) và bảng câu hỏi sức khỏe bệnh nhân 9 mục (PHQ-9). Điểm GAD-7 và PHQ-9 có tương quan với điểm chức năng sức khỏe thể chất và tinh thần nói chung, chất lượng cuộc sống liên quan đến thị lực, sự đau khổ liên quan đến ngoại hình, chức năng xã hội liên quan đến ngoại hình và các yếu tố sinh học xã hội khác. KẾT QUẢ Năm bệnh nhân (2%) đã được chẩn đoán trước là mắc chứng rối loạn lo âu và 20 bệnh nhân (7%) đã được chẩn đoán trước là mắc chứng trầm cảm. Tuy nhiên, quá trình sàng lọc của chúng tôi cho thấy 26 bệnh nhân (9%) có các triệu chứng lo âu, 31 bệnh nhân (11%) có các triệu chứng trầm cảm và 40 bệnh nhân (14%) mắc cả triệu chứng lo âu và trầm cảm. Việc chẩn đoán thiếu này đối với cả rối loạn lo âu và trầm cảm là có ý nghĩa (lần lượt là p < 0,001). Điểm GAD-7 cao hơn có liên quan đáng kể đến điểm PHQ-9 cao hơn, chức năng xã hội liên quan đến ngoại hình thấp hơn, chức năng sức khỏe tinh thần thấp hơn và giới tính nữ (lần lượt là p ≤ 0,021). Điểm PHQ-9 cao hơn có liên quan đáng kể đến chức năng sức khỏe thể chất và tinh thần thấp hơn, trình độ học vấn cao hơn và mất thị lực không do chấn thương (lần lượt là p ≤ 0,038). KẾT LUẬN Các rối loạn lo âu và trầm cảm có vẻ như được chẩn đoán thiếu ở những người đeo mắt giả và có tỷ lệ mắc cao hơn so với dân số nói chung. Do đó, nên thường xuyên thực hiện sàng lọc trắc nghiệm tâm lý trong quá trình chăm sóc lâm sàng. Đối với quá trình phục hồi chức năng lâu dài thành công, việc chăm sóc tích hợp của một nhóm đa ngành bao gồm các bác sĩ phẫu thuật tạo hình mắt, bác sĩ nhãn khoa, bác sĩ nhãn khoa, bác sĩ đa khoa và bác sĩ tâm lý là điều cần thiết."} +{"idx": 1870579, "en": "SARS-CoV-2, which led to the 2020 global pandemic, is responsible for the Coronavirus Disease 2019 (COVID-19), a respiratory illness, and presents a tropism for the central nervous system. Like most members of this family, the virus is composed of structural and non-structural proteins (NSPs). The non-structural proteins are critical elements of the replication and transcription complex (RTC), as well as immune system evasion. Through hijacking the endoplasmic reticulum (ER) membrane, NSPs help the virus establish the RTC, inducing ER stress after membrane rearrangement and causing severe neuronal disturbance. In this review, we focus on the role of Nsp3, 4, and 6 in intracellular membrane rearrangement and evaluate the potential disruption of the central nervous system and the neurodegeneration which it could trigger. Studies of these NSPs will not only bring to light their specific role in viral infection but also facilitate the discovery of novel targeted drugs.", "gemini": "SARS-CoV-2, nguyên nhân gây ra đại dịch toàn cầu năm 2020, gây ra Bệnh do virus Corona 2019 (COVID-19), một bệnh về đường hô hấp và có ái tính với hệ thần kinh trung ương. Giống như hầu hết các thành viên trong họ này, virus bao gồm các protein cấu trúc và không cấu trúc (NSP). Các protein không cấu trúc là các yếu tố quan trọng của phức hợp sao chép và phiên mã (RTC), cũng như khả năng trốn tránh hệ thống miễn dịch. Thông qua việc chiếm giữ màng lưới nội chất (ER), NSP giúp virus thiết lập RTC, gây ra stress ER sau khi sắp xếp lại màng và gây ra rối loạn thần kinh nghiêm trọng. Trong bài tổng quan này, chúng tôi tập trung vào vai trò của Nsp3, 4 và 6 trong việc sắp xếp lại màng nội bào và đánh giá sự gián đoạn tiềm ẩn của hệ thần kinh trung ương và thoái hóa thần kinh mà nó có thể gây ra. Các nghiên cứu về các NSP này không chỉ làm sáng tỏ vai trò cụ thể của chúng trong nhiễm virus mà còn thúc đẩy việc phát hiện ra các loại thuốc đích mới."} +{"idx": 1870580, "en": "PURPOSE Bronchoalveolar lavage and transbronchial biopsy can be a useful tool in the evaluation of interstitial lung disease (ILD), but patient selection for this procedure remains poorly defined. Determining clinical characteristics that help with patient selection for bronchoscopy may improve confidence of ILD classification while limiting potential adverse outcomes associated with surgical lung biopsy. The purpose of this study is to identify factors that were associated with change in multidisciplinary ILD diagnosis (MDD) before and after incorporation of BAL and TBBx data. METHODS We conducted a retrospective cohort study of ILD patients at a single center who underwent bronchoscopy in the diagnostic workup of ILD. We performed sequential MDD both pre- and post-bronchoscopy to calculate the frequency of change in diagnosis after incorporating information from BAL and TBBx and identify features associated with change in diagnosis. RESULTS 245 patients were included in the study. Bronchoscopy led to a change in diagnosis in 58 patients (23.7%). The addition of TBBx to BAL increased diagnostic yield from 21.8 to 34.1% (p = 0.027). Identification of antigen, HRCT scan inconsistent with UIP, and absence of a pre-bronchoscopy diagnosis of CTD-ILD or IPAF were associated with a change in diagnosis after bronchoscopy. CONCLUSION Our study suggests clinical features that may assist with patient selection for bronchoscopy. We suggest bronchoscopy in patients with identified antigen or an HRCT that is consistent with a non-IPF diagnosis. Appropriate patient selection for bronchoscopy may improve ILD diagnostic confidence and avoid potential complications from more invasive and higher risk procedures.", "gemini": "MỤC ĐÍCH Rửa phế nang và sinh thiết phế quản có thể là một công cụ hữu ích trong việc đánh giá bệnh phổi kẽ (ILD), nhưng việc lựa chọn bệnh nhân cho thủ thuật này vẫn chưa được xác định rõ. Việc xác định các đặc điểm lâm sàng giúp lựa chọn bệnh nhân cho nội soi phế quản có thể cải thiện độ tin cậy của phân loại ILD trong khi hạn chế các kết quả bất lợi tiềm ẩn liên quan đến sinh thiết phổi phẫu thuật. Mục đích của nghiên cứu này là xác định các yếu tố liên quan đến thay đổi trong chẩn đoán ILD đa ngành (MDD) trước và sau khi kết hợp dữ liệu BAL và TBBx. PHƯƠNG PHÁP Chúng tôi đã tiến hành một nghiên cứu theo dõi hồi cứu đối với bệnh nhân ILD tại một trung tâm duy nhất đã nội soi phế quản trong quá trình chẩn đoán ILD. Chúng tôi đã thực hiện MDD tuần tự trước và sau nội soi phế quản để tính tần suất thay đổi chẩn đoán sau khi kết hợp thông tin từ BAL và TBBx và xác định các đặc điểm liên quan đến thay đổi chẩn đoán. KẾT QUẢ 245 bệnh nhân được đưa vào nghiên cứu. Nội soi phế quản dẫn đến thay đổi chẩn đoán ở 58 bệnh nhân (23,7%). Việc bổ sung TBBx vào BAL làm tăng tỷ lệ chẩn đoán từ 21,8 lên 34,1% (p = 0,027). Việc xác định kháng nguyên, chụp HRCT không phù hợp với UIP và không có chẩn đoán CTD-ILD hoặc IPAF trước nội soi phế quản có liên quan đến thay đổi chẩn đoán sau nội soi phế quản. KẾT LUẬN Nghiên cứu của chúng tôi chỉ ra các đặc điểm lâm sàng có thể hỗ trợ lựa chọn bệnh nhân cho nội soi phế quản. Chúng tôi đề xuất nội soi phế quản ở những bệnh nhân có kháng nguyên được xác định hoặc HRCT phù hợp với chẩn đoán không phải IPF. Việc lựa chọn bệnh nhân phù hợp cho nội soi phế quản có thể cải thiện độ tin cậy chẩn đoán ILD và tránh các biến chứng tiềm ẩn từ các thủ thuật xâm lấn hơn và có nguy cơ cao hơn."} +{"idx": 1870581, "en": "Carbon dots and gold nanoclusters co-encapsulated by zeolitic imidazolate framework-8 (CDs/AuNCs@ZIF-8) have been obtained at room temperature. The composite has been applied to the ratiometric fluorescence determination of mercury(II). The composite shows fluorescence emission maxima at 440 and 640 nm under 360 nm excitation, due to the CDs and AuNCs, respectively (associated quantum yields were 18% and 17%, respectively). In the presence of Hg2+, the fluorescence at about 640 nm is quenched, while the fluorescence at about 440 nm is unaffected. The CDs/AuNCs@ZIF-8 composite allows the sensitive detection of Hg2+, with the fluorescence intensity ratio (I640/I440) decreasing linearly with Hg2+ concentration over the range 3-30 nM. The fluorescence emission of the composite changes color from red to blue with increasing Hg2+ under UV excitation, which can easily be discerned visually. This visual detection of Hg2+ is due to the high fluorescence quantum yields of the CDs and AuNCs and the ~ 200 nm separation between the two emission maxima. Graphical abstract (A) Schematic diagram showing the operating principle of the determination for Hg(II). (B) Digital graph of the solutions in absence and presence of 30 nM Hg(II) under a portable UV lamp.", "gemini": "Các chấm carbon và cụm nano vàng được bao bọc đồng thời bởi khung zeolit ​​imidazolate-8 (CDs/AuNCs@ZIF-8) đã thu được ở nhiệt độ phòng. Hợp chất này đã được ứng dụng để xác định huỳnh quang tỷ lệ của thủy ngân(II). Hợp chất này cho thấy cực đại phát xạ huỳnh quang ở 440 và 640 nm dưới sự kích thích 360 nm, lần lượt là do các chấm carbon và cụm nano vàng (lần lượt là lượng tử phát xạ liên quan là 18% và 17%). Khi có mặt Hg2+, huỳnh quang ở khoảng 640 nm bị dập tắt, trong khi huỳnh quang ở khoảng 440 nm không bị ảnh hưởng. Hợp chất CDs/AuNCs@ZIF-8 cho phép phát hiện Hg2+ nhạy, với tỷ lệ cường độ huỳnh quang (I640/I440) giảm tuyến tính theo nồng độ Hg2+ trong phạm vi 3-30 nM. Phát xạ huỳnh quang của hợp chất thay đổi màu từ đỏ sang xanh lam khi tăng Hg2+ dưới sự kích thích tia cực tím, có thể dễ dàng phân biệt bằng mắt thường. Phát hiện trực quan Hg2+ này là do lượng tử huỳnh quang cao của các chấm carbon và cụm nano vàng và khoảng cách ~200 nm giữa hai cực đại phát xạ. Tóm tắt đồ họa (A) Sơ đồ nguyên lý hoạt động của nguyên tắc xác định Hg(II). (B) Đồ thị kỹ thuật số của các dung dịch khi không có và có 30 nM Hg(II) dưới đèn UV cầm tay."} +{"idx": 1870582, "en": "PURPOSE OF REVIEW Statins are first-line therapy for lowering low-density lipoprotein (LDL) cholesterol in familial hypercholesterolemia (FH), particularly in heterozygous patients. We review advances and new questions on the use of statins in FH. RECENT FINDINGS Cumulative evidence from registry data and sub-analyses of clinical trials mandates the value of statin therapy for prevention of atherosclerotic cardiovascular disease (ASCVD) in FH. Statins are safe in children and adolescents with FH, with longer term cardiovascular benefits. The potentially toxic effects of statins in pregnancy need to be considered, but no association has been reported in prospective cohort studies with birth defects. There is no rationale for discontinuation of statins in elderly FH unless indicated by adverse events. FH is undertreated, with > 80% of statin-treated FH patients failing to attain LDL cholesterol treatment targets. This may relate to adherence, tolerability, and genetic differences in statin responsiveness. Statin treatment from childhood may reduce the need for stringent cholesterol targets. Combination of statins with ezetimibe and PCSK9 inhibitors significantly improves the efficacy of treatment. Whether statin use could improve the clinical course of FH patients with COVID-19 and other respiratory infections remains an unsolved issue for future research. Statins are the mainstay for primary and secondary prevention of ASCVD in FH. Sustained long-term optimal statin treatment from an early age can effectively prevent ASCVD over decades of life. Despite their widespread use, statins merit further investigation in FH.", "gemini": "MỤC ĐÍCH CỦA BÀI ĐÁNH GIÁ Statin là liệu pháp đầu tay để làm giảm cholesterol lipoprotein tỷ trọng thấp (LDL) ở tăng cholesterol máu gia đình (FH), đặc biệt ở những bệnh nhân dị hợp tử. Chúng tôi xem xét những tiến bộ và câu hỏi mới về việc sử dụng statin trong FH. NHỮNG PHÁT HIỆN GẦN ĐÂY Bằng chứng tích lũy từ dữ liệu đăng ký và phân tích phụ của các thử nghiệm lâm sàng bắt buộc phải có giá trị của liệu pháp statin để phòng ngừa bệnh tim mạch do xơ vữa động mạch (ASCVD) ở FH. Statin an toàn ở trẻ em và thanh thiếu niên mắc FH, với lợi ích về tim mạch lâu dài hơn. Cần xem xét các tác dụng độc hại tiềm tàng của statin trong thai kỳ, nhưng chưa có báo cáo về mối liên quan nào trong các nghiên cứu đoàn hệ triển vọng với các dị tật bẩm sinh. Không có lý do gì để ngừng dùng statin ở người cao tuổi mắc FH trừ khi có các tác dụng phụ. FH được điều trị chưa đủ, với> 80% bệnh nhân FH được điều trị bằng statin không đạt được mục tiêu điều trị cholesterol LDL. Điều này có thể liên quan đến tính tuân thủ, khả năng dung nạp và sự khác biệt về mặt di truyền trong khả năng đáp ứng với statin. Điều trị bằng statin từ thời thơ ấu có thể làm giảm nhu cầu về mục tiêu cholesterol nghiêm ngặt. Sự kết hợp statin với ezetimibe và chất ức chế PCSK9 cải thiện đáng kể hiệu quả điều trị. Việc sử dụng statin có thể cải thiện diễn biến lâm sàng của bệnh nhân FH mắc COVID-19 và các bệnh nhiễm trùng đường hô hấp khác hay không vẫn là một vấn đề chưa được giải quyết đối với nghiên cứu trong tương lai. Statin là trụ cột cho việc phòng ngừa tiên phát và thứ phát ASCVD ở FH. Điều trị bằng statin tối ưu lâu dài liên tục từ khi còn nhỏ có thể ngăn ngừa ASCVD hiệu quả trong nhiều thập kỷ của cuộc đời. Mặc dù được sử dụng rộng rãi, statin vẫn cần được nghiên cứu thêm ở FH."} +{"idx": 1870583, "en": "We describe a human and large animal Langendorff experimental apparatus for live electrophysiological studies and measure the electrophysiological changes due to gap junction uncoupling in human and porcine hearts. The resultant ex vivo intact human and porcine model can bridge the translational gap between smaller simple laboratory models and clinical research. In particular, electrophysiological models would benefit from the greater myocardial mass of a large heart due to its effects on far-field signal, electrode contact issues and motion artefacts, consequently more closely mimicking the clinical setting. Porcine (n = 9) and human (n = 4) donor hearts were perfused on a custom-designed Langendorff apparatus. Epicardial electrograms were collected at 16 sites across the left atrium and left ventricle. A total of 1 mM of carbenoxolone was administered at 5 ml/min to induce cellular uncoupling, and then recordings were repeated at the same sites. Changes in electrogram characteristics were analysed. We demonstrate the viability of a controlled ex vivo model of intact porcine and human hearts for electrophysiology with pharmacological modulation. Carbenoxolone reduces cellular coupling and changes contact electrogram features. The time from stimulus artefact to (-dV/dt)max increased between baseline and carbenoxolone (47.9 ± 4.1-67.2 ± 2.7 ms) indicating conduction slowing. The features with the largest percentage change between baseline and carbenoxolone were fractionation + 185.3%, endpoint amplitude - 106.9%, S-endpoint gradient + 54.9%, S point - 39.4%, RS ratio + 38.6% and (-dV/dt)max - 20.9%. The physiological relevance of this methodological tool is that it provides a model to further investigate pharmacologically induced pro-arrhythmic substrates.", "gemini": "Chúng tôi mô tả một thiết bị thực nghiệm Langendorff của động vật lớn và con người để nghiên cứu điện sinh lý trực tiếp và đo các thay đổi điện sinh lý do sự tách ghép liên hợp khe hở ở tim người và tim lợn. Mô hình nguyên vẹn ex vivo của người và lợn thu được có thể thu hẹp khoảng cách chuyển dịch giữa các mô hình phòng thí nghiệm đơn giản nhỏ hơn và nghiên cứu lâm sàng. Đặc biệt, các mô hình điện sinh lý sẽ được hưởng lợi từ khối lượng cơ tim lớn hơn của một trái tim lớn do ảnh hưởng của nó đến tín hiệu trường xa, các vấn đề tiếp xúc điện cực và các hiện vật chuyển động, do đó mô phỏng chặt chẽ hơn bối cảnh lâm sàng. Tim của người hiến tặng (n = 9) và tim của lợn (n = 4) được tưới máu trên một thiết bị Langendorff được thiết kế riêng. Điện tâm đồ ngoài màng tim được thu thập tại 16 vị trí trên tâm nhĩ trái và tâm thất trái. Tổng cộng 1 mM carbenoxolone được dùng với liều 5 ml/phút để gây ra sự tách ghép tế bào, sau đó lặp lại các ghi chép ở cùng các vị trí đó. Các thay đổi về đặc điểm điện tâm đồ đã được phân tích. Chúng tôi chứng minh tính khả thi của một mô hình ex vivo có kiểm soát của tim người và lợn nguyên vẹn để nghiên cứu điện sinh lý với sự điều chỉnh dược lý. Carbenoxolone làm giảm sự ghép nối tế bào và thay đổi các đặc điểm điện tâm đồ tiếp xúc. Thời gian từ hiện vật kích thích đến (-dV/dt)max tăng lên giữa đường cơ sở và carbenoxolone (47,9 ± 4,1-67,2 ± 2,7 ms) cho thấy sự dẫn truyền chậm lại. Các đặc điểm có tỷ lệ phần trăm thay đổi lớn nhất giữa đường cơ sở và carbenoxolone là phân đoạn + 185,3%, biên độ điểm cuối - 106,9%, độ dốc điểm cuối S + 54,9%, điểm S - 39,4%, tỷ lệ RS + 38,6% và (-dV/dt)max - 20,9%. Tầm quan trọng về mặt sinh lý của công cụ phương pháp luận này là nó cung cấp một mô hình để nghiên cứu sâu hơn các chất nền gây loạn nhịp do dược lý gây ra."} +{"idx": 1870584, "en": "A ratiometric fluorescence assay for glutathione (GSH) was developed. The novel assay is based on a nanoprobe composed of manganese dioxide nanosheets (MnO2 NS) and dual-emission carbon dots (de-CDs) with intrinsic GSH-response property. After construction of the nanoprobe, two emission peaks of de-CDs were suppressed to varying degrees by MnO2 NS. The suppression was relieved and the two emission peaks recovered proportionally when MnO2 NS was decomposed by GSH, thus realizing the ratiometric assay for micromolar GSH. The intrinsic responsiveness of de-CDs to millimolar GSH broadens the analytical range of the nanoprobe. An appropriate precursor, calcon-carboxylic acid, was screened out to synthesize de-CDs via one-step hydrothermal treatment. The de-CD@MnO2 NS nanoprobe can measure GSH concentrations through the fluorescence intensity ratio between 435 and 516 nm excited at 365 nm. The range of response was from 1 μM to 10 mM and the detection limit reached 0.6 μM (3σ criterion). Benefiting from its good biocompatibility, the proposed nanoprobe has excellent applicability for intracellular GSH imaging.Graphical abstract Schematic representation of glutathione (GSH) ratiometric detection. The nanoprobe is prepared from dual-emission carbon dots (de-CDs) and manganese dioxide nanosheets (MnO2 NS). GSH removes quenching effect by decomposing MnO2 NS and induces intrinsic response of de-CDs, which realizes ratiometric detection.", "gemini": "Một xét nghiệm huỳnh quang tỷ lệ cho glutathione (GSH) đã được phát triển. Xét nghiệm mới dựa trên nanoprobe gồm các phiến nano dioxide mangan (MnO2 NS) và các chấm cacbon phát xạ kép (de-CD) có đặc tính phản ứng với GSH nội tại. Sau khi chế tạo nanoprobe, hai đỉnh phát xạ của de-CD đã bị MnO2 NS ức chế ở các mức độ khác nhau. Sự ức chế được giải tỏa và hai đỉnh phát xạ phục hồi theo tỷ lệ khi MnO2 NS bị GSH phân hủy, do đó nhận ra xét nghiệm tỷ lệ cho GSH micromolar. Khả năng đáp ứng nội tại của de-CD đối với GSH milimolar mở rộng phạm vi phân tích của nanoprobe. Một tiền chất thích hợp, axit calcon-cacboxylic, đã được sàng lọc để tổng hợp de-CD thông qua xử lý thủy nhiệt một bước. Nanoprobe de-CD@MnO2 NS có thể đo nồng độ GSH thông qua tỷ lệ cường độ huỳnh quang giữa 435 và 516 nm được kích thích ở 365 nm. Phạm vi đáp ứng từ 1 μM đến 10 mM và giới hạn phát hiện đạt 0,6 μM (tiêu chuẩn 3σ). Nhờ khả năng tương thích sinh học tốt, nanoprobe được đề xuất có khả năng ứng dụng tuyệt vời cho hình ảnh GSH trong tế bào.Tóm tắt đồ họa Biểu diễn sơ đồ phát hiện tỷ lệ glutathione (GSH). Nanoprobe được chế tạo từ các chấm cacbon phát xạ kép (de-CD) và các phiến nano dioxide mangan (MnO2 NS). GSH loại bỏ tác dụng làm tắt bằng cách phân hủy MnO2 NS và gây ra phản ứng nội tại của de-CD, giúp nhận ra phát hiện tỷ lệ."} +{"idx": 1870585, "en": "The glial cell line-derived neurotrophic factor (GDNF) and its canonical receptor Ret can signal both in tandem and separately to exert many vital functions in the midbrain dopamine system. It is known that Ret has effects on maintenance, physiology, protection and regeneration in the midbrain dopamine system, with the physiological functions of GDNF still somewhat unclear. Notwithstanding, Ret ligands, such as GDNF, are considered as promising candidates for neuroprotection and/or regeneration in Parkinson's disease, although data from clinical trials are so far inconclusive. In this review, we discuss the current knowledge of GDNF/Ret signaling in the dopamine system in vivo as well as crosstalk with pathology-associated proteins and their signaling in mammals.", "gemini": "Yếu tố dinh dưỡng thần kinh có nguồn gốc từ dòng tế bào thần kinh đệm (GDNF) và thụ thể Ret chính thống của nó có thể truyền tín hiệu cả song song và riêng biệt để thực hiện nhiều chức năng quan trọng trong hệ thống dopamine ở não giữa. Người ta biết rằng Ret có tác dụng duy trì, sinh lý, bảo vệ và tái tạo trong hệ thống dopamine ở não giữa, trong khi các chức năng sinh lý của GDNF vẫn chưa rõ ràng. Mặc dù vậy, các phối tử Ret, chẳng hạn như GDNF, được coi là những ứng cử viên đầy hứa hẹn cho việc bảo vệ thần kinh và/hoặc tái tạo trong bệnh Parkinson, mặc dù dữ liệu từ các thử nghiệm lâm sàng cho đến nay vẫn chưa có kết luận. Trong bài tổng quan này, chúng tôi thảo luận về kiến thức hiện tại về tín hiệu GDNF/Ret trong hệ thống dopamine ở động vật sống cũng như sự tương tác với các protein liên quan đến bệnh lý và tín hiệu của chúng ở động vật có vú."} +{"idx": 1870586, "en": "Maternal psychosocial stress may impact child neurodevelopment, but little is known regarding psychosocial job strain. We hypothesized high psychosocial job strain during pregnancy was associated with behavioural problems in the 11-year-old children. Mothers in the Danish National Birth Cohort (1996-2002) were included if they worked, provided information on job strain [Karasek's model: high job strain (often job demand/seldom job control) and passive (seldom or sometimes job demands/seldom job control)] during early pregnancy. At the 11-year follow-up, children (N = 30,592), mothers (N = 30,993), and teachers (N = 12,810) responded to the Strength and Difficulties Questionnaire (SDQ), a screening tool for child behaviour. Scores for hyperactivity, conduct, emotional and peer problems were dichotomised [80% (no) vs. 20% (yes)] according to Danish norms (yes/no). Maternal job strain was not associated with behavioural problems with teachers as informants. When assessed by child or mother, high maternal job strain increased risk of child behavioural problems, but risks were more pronounced for mothers in passive jobs [maternal assessment of total difficulties/odds ratio (95% confidence interval): high strain-girls: 1.16 (0.97-1.40); boys: 1.24 (1.02-1.50). Passive girls: 1.43 (1.21-1.68); boys: 1.25 (1.05-1.49)]. This is one of the first studies on this topic. The different types of maternal job strain were partly associated with child behavioural problems at 11 years; more so if mothers worked in passive rather than the hypothesized high strain jobs. Findings showing dependency on informant could not only indicate unmeasured confounding or rater's bias, but also selection in the smaller numbers of teacher informants or different environments of interaction with the children.", "gemini": "Căng thẳng tâm lý xã hội của bà mẹ có thể ảnh hưởng đến sự phát triển thần kinh của trẻ, nhưng người ta biết rất ít về căng thẳng nghề nghiệp do tâm lý xã hội. Chúng tôi giả thuyết rằng căng thẳng nghề nghiệp do tâm lý xã hội cao trong thời kỳ mang thai có liên quan đến các vấn đề về hành vi ở trẻ em 11 tuổi. Những bà mẹ trong Đội nghiên cứu sinh quốc gia Đan Mạch (1996-2002) được đưa vào nghiên cứu nếu họ làm việc, cung cấp thông tin về căng thẳng nghề nghiệp [mô hình của Karasek: căng thẳng nghề nghiệp cao (thường là nhu cầu công việc/hiếm khi kiểm soát công việc) và thụ động (hiếm khi hoặc đôi khi nhu cầu công việc/hiếm khi kiểm soát công việc)] trong thời kỳ đầu mang thai. Khi theo dõi đến năm 11 tuổi, trẻ em (N = 30.592), bà mẹ (N = 30.993) và giáo viên (N = 12.810) đã trả lời Bản câu hỏi về sức mạnh và khó khăn (SDQ), một công cụ sàng lọc hành vi của trẻ. Điểm số về tăng động, hành vi, cảm xúc và các vấn đề về bạn bè được phân đôi [80% (không) so với 20% (có)] theo các chuẩn mực của Đan Mạch (có/không). Căng thẳng nghề nghiệp của bà mẹ không liên quan đến các vấn đề về hành vi với giáo viên là người cung cấp thông tin. Khi được trẻ hoặc mẹ đánh giá, căng thẳng nghề nghiệp cao của bà mẹ làm tăng nguy cơ trẻ gặp vấn đề về hành vi, nhưng rủi ro này rõ rệt hơn đối với những bà mẹ làm công việc thụ động [đánh giá tổng thể của bà mẹ về khó khăn/tỷ lệ nguy cơ (khoảng tin cậy 95%): căng thẳng cao-con gái: 1,16 (0,97-1,40); con trai: 1,24 (1,02-1,50). Con gái thụ động: 1,43 (1,21-1,68); con trai: 1,25 (1,05-1,49)]. Đây là một trong những nghiên cứu đầu tiên về chủ đề này. Các loại căng thẳng nghề nghiệp khác nhau của bà mẹ có liên quan một phần đến các vấn đề về hành vi của trẻ ở độ tuổi 11; nhiều hơn nếu bà mẹ làm công việc thụ động thay vì công việc căng thẳng cao theo giả thuyết. Những phát hiện cho thấy sự phụ thuộc vào người cung cấp thông tin không chỉ có thể chỉ ra sự nhiễu loạn chưa được đo lường hoặc sự thiên vị của người đánh giá, mà còn cả sự lựa chọn trong số lượng người cung cấp thông tin là giáo viên ít hơn hoặc các môi trường tương tác khác nhau với trẻ em."} +{"idx": 1870587, "en": "PURPOSE Deoxycholic acid (DCA), a secondary bile acid, is reportedly increased in the serum of patients with nonalcoholic steatohepatitis and animals with experimentally induced hepatocellular carcinoma (HCC), but its contribution to malignant behaviors of HCC has not been precisely clarified. This study aimed to examine the effect of DCA on hepatic stellate cells (HSCs), a major component of nonparenchymal cells in the liver, and its subsequent indirect effect on HCC cells. METHODS LX2 cells, a human HSC line, were treated with DCA in vitro. Then, HuH7 cells, a human hepatoma cell line, were incubated in conditioned media of DCA-treated LX2 to investigate the subsequent effect focusing on malignant behaviors. RESULTS DCA resulted in cellular senescence in LX2 with the decreased cell proliferation via cell cycle arrest at G0/1 phase, together with the induction of senescence-associated secretory phenotype (SASP) factors. To investigate the influence of SASP factors secreted by HSCs in response to DCA, HCC cells were treated with conditioned media that promoted cell migration and invasion via induction of epithelial mesenchymal transition. These changes were attenuated in the presence of neutralizing antibody against IL8 or TGFβ. Pathological analysis of surgical specimens from HCC patients revealed that senescent HSCs were detected in the stroma surrounding HCC. CONCLUSION Our data suggest an important role of HSC senescence caused by DCA for the malignant biological behaviors of HCC via induction of SASP factors, particularly IL8 and TGFβ.", "gemini": "MỤC ĐÍCH Axit deoxycholic (DCA), một axit mật thứ cấp, được báo cáo là tăng trong huyết thanh của bệnh nhân mắc bệnh gan nhiễm mỡ không do rượu và động vật bị ung thư biểu mô tế bào gan (HCC) do thực nghiệm gây ra, nhưng sự đóng góp của nó vào các hành vi ác tính của HCC vẫn chưa được làm rõ chính xác. Nghiên cứu này nhằm mục đích kiểm tra tác dụng của DCA đối với các tế bào sao gan (HSC), một thành phần chính của các tế bào không nhu mô trong gan và tác dụng gián tiếp sau đó của nó đối với các tế bào HCC. PHƯƠNG PHÁP Các tế bào LX2, một dòng HSC của người, được xử lý bằng DCA trong ống nghiệm. Sau đó, các tế bào HuH7, một dòng tế bào gan của người, được ủ trong môi trường có điều kiện của LX2 đã xử lý bằng DCA để nghiên cứu tác dụng tiếp theo tập trung vào các hành vi ác tính. KẾT QUẢ DCA dẫn đến tình trạng lão hóa tế bào ở LX2 với sự giảm sinh sản tế bào thông qua sự bắt giữ chu kỳ tế bào ở pha G0/1, cùng với sự cảm ứng các yếu tố kiểu hình tiết ra liên quan đến lão hóa (SASP). Để nghiên cứu ảnh hưởng của các yếu tố SASP do HSC tiết ra đáp ứng với DCA, các tế bào HCC được xử lý bằng môi trường có điều kiện thúc đẩy quá trình di chuyển và xâm lấn tế bào thông qua quá trình cảm ứng chuyển đổi biểu mô trung mô. Những thay đổi này được giảm bớt khi có sự hiện diện của kháng thể trung hòa chống lại IL8 hoặc TGFβ. Phân tích bệnh lý các mẫu phẫu thuật từ bệnh nhân HCC cho thấy các HSC lão hóa được phát hiện trong lớp đệm xung quanh HCC. KẾT LUẬN Dữ liệu của chúng tôi cho thấy vai trò quan trọng của sự lão hóa HSC do DCA gây ra đối với các hành vi sinh học ác tính của HCC thông qua quá trình cảm ứng các yếu tố SASP, đặc biệt là IL8 và TGFβ."} +{"idx": 1870588, "en": "Automatic grading of retinal blood vessels from fundus image can be a useful tool for diagnosis, planning and treatment of eye. Automatic diagnosis of retinal images for early detection of glaucoma, stroke, and blindness is emerging in intelligent health care system. The method primarily depends on various abnormal signs, such as area of hard exudates, area of blood vessels, bifurcation points, texture, and entropies. The development of an automated screening system based on vessel width, tortuosity, and vessel branching are also used for grading. However, the automated method that directly can come to a decision by taking the fundus images got less attention. Detecting eye problems based on the tortuosity of the vessel from fundus images is a complicated task for opthalmologists. So automated grading algorithm using deep learning can be most valuable for grading retinal health. The aim of this work is to develop an automatic computer aided diagnosis system to solve the problem. This work approaches to achieve an automatic grading method that is opted using Convolutional Neural Network (CNN) model. In this work we have studied the state-of-the-art machine learning algorithms and proposed an attention network which can grade retinal images. The proposed method is validated on a public dataset EIARG1, which is only publicly available dataset for such task as per our knowledge.", "gemini": "Việc phân loại tự động các mạch máu võng mạc từ hình ảnh đáy mắt có thể là một công cụ hữu ích để chẩn đoán, lập kế hoạch và điều trị mắt. Chẩn đoán tự động hình ảnh võng mạc để phát hiện sớm bệnh tăng nhãn áp, đột quỵ và mù lòa đang nổi lên trong hệ thống chăm sóc sức khỏe thông minh. Phương pháp này chủ yếu dựa trên các dấu hiệu bất thường khác nhau, chẳng hạn như diện tích của chất tiết cứng, diện tích mạch máu, điểm phân đôi, kết cấu và entropy. Việc phát triển một hệ thống sàng lọc tự động dựa trên chiều rộng mạch, độ ngoằn ngoèo và nhánh mạch cũng được sử dụng để phân loại. Tuy nhiên, phương pháp tự động có thể đưa ra quyết định trực tiếp bằng cách lấy hình ảnh đáy mắt ít được chú ý hơn. Phát hiện các vấn đề về mắt dựa trên độ ngoằn ngoèo của mạch máu từ hình ảnh đáy mắt là một nhiệm vụ phức tạp đối với các bác sĩ nhãn khoa. Vì vậy, thuật toán phân loại tự động sử dụng học sâu có thể có giá trị nhất để phân loại sức khỏe võng mạc. Mục đích của công trình này là phát triển một hệ thống chẩn đoán hỗ trợ máy tính tự động để giải quyết vấn đề. Công trình này tiếp cận để đạt được một phương pháp phân loại tự động được lựa chọn bằng mô hình Mạng nơ-ron tích chập (CNN). Trong công trình này, chúng tôi đã nghiên cứu các thuật toán học máy tiên tiến và đề xuất một mạng chú ý có thể phân loại hình ảnh võng mạc. Phương pháp được đề xuất được xác thực trên một tập dữ liệu công khai EIARG1, đây là tập dữ liệu chỉ có sẵn công khai cho nhiệm vụ như vậy theo hiểu biết của chúng tôi."} +{"idx": 1870589, "en": "OBJECTIVES To evaluate whether surface characteristics of different titanium modifications may influence the composition of the salivary pellicle on each surface by analyzing the salivary proteome through mass spectrometry-based proteomics. MATERIALS AND METHODS Titanium discs with three surfaces modifications (PT (machined titanium), SLA (sandblasted/large-grit/acid-etched), and SLActive (modified SLA)) were characterized (topography, chemistry, and energy) prior to being exposed to saliva for 2 h to form a protein pellicle. The resultant protein layer was retrieved and analyzed through mass spectrometry (nLC-ESI-MS/MS) to examine the surface specificity for protein binding, while the proteome profile of each surface was classified. RESULTS The proteome analysis showed that the salivary pellicle composition was more complex on rough surfaces (SLA and SLActive). Although variability in protein composition was observed between surfaces, most proteins were detected on more than one surface, indicating a limited surface specificity for protein binding. Additionally, the salivary pellicle formed on the SLActive presented a larger number of proteins associated with immune response, biological adhesion, and biomineralization. CONCLUSIONS Although topography, chemistry, and energy differed between the surfaces, they were not determinant to produce a salivary pellicle with high surface specificity. Also, we showed that several salivary proteins adsorbed on Ti surfaces are involved in biological functions important to the biointegration. CLINICAL RELEVANCE This study sheds light on the necessity for the development of bioactive surfaces that favors the formation of a specific protein layer that can enhance tissue response to assist the biointegration of dental implants.", "gemini": "MỤC TIÊU Đánh giá liệu các đặc điểm bề mặt của các sửa đổi titan khác nhau có thể ảnh hưởng đến thành phần của màng mỏng nước bọt trên mỗi bề mặt hay không bằng cách phân tích proteome nước bọt thông qua proteomics dựa trên phổ khối. VẬT LIỆU VÀ PHƯƠNG PHÁP Các đĩa titan có ba sửa đổi bề mặt (PT (titan gia công), SLA (thổi cát/hạt lớn/ăn mòn axit) và SLActive (SLA đã sửa đổi)) được đặc trưng (địa hình, hóa học và năng lượng) trước khi tiếp xúc với nước bọt trong 2 giờ để tạo thành một màng mỏng protein. Lớp protein kết quả được lấy ra và phân tích thông qua phổ khối (nLC-ESI-MS/MS) để kiểm tra tính đặc hiệu của bề mặt đối với liên kết protein, trong khi hồ sơ proteome của mỗi bề mặt được phân loại. KẾT QUẢ Phân tích proteome cho thấy thành phần màng mỏng nước bọt phức tạp hơn trên bề mặt gồ ghề (SLA và SLActive). Mặc dù sự thay đổi về thành phần protein được quan sát thấy giữa các bề mặt, hầu hết các protein được phát hiện trên nhiều hơn một bề mặt, chỉ ra tính đặc hiệu của bề mặt hạn chế đối với liên kết protein. Ngoài ra, màng mỏng nước bọt hình thành trên SLActive có số lượng lớn hơn các protein liên quan đến phản ứng miễn dịch, kết dính sinh học và khoáng hóa sinh học. KẾT LUẬN Mặc dù địa hình, hóa học và năng lượng khác nhau giữa các bề mặt, nhưng chúng không phải là yếu tố quyết định để tạo ra một màng mỏng nước bọt có tính đặc hiệu bề mặt cao. Ngoài ra, chúng tôi đã chỉ ra rằng một số protein nước bọt hấp phụ trên bề mặt Ti có liên quan đến các chức năng sinh học quan trọng đối với sự tích hợp sinh học. TÍNH LIÊN QUAN LÂM SÀNG Nghiên cứu này làm sáng tỏ sự cần thiết phải phát triển các bề mặt hoạt tính sinh học có lợi cho việc hình thành một lớp protein cụ thể có thể tăng cường phản ứng của mô để hỗ trợ sự tích hợp sinh học của cấy ghép nha khoa."} +{"idx": 1870590, "en": "Advances in the diagnosis and management of congenital heart disease (CHD) have resulted in a growing population of patients surviving well into adulthood and requiring lifelong follow-up. Flow quantification is a central component in the assessment of patients with CHD. 4D flow magnetic resonance imaging (MRI) has emerged as a tool that enables comprehensive study of flow. It involves the acquisition of a three-dimensional time-resolved volume with velocity encoding in all three spatial directions along the cardiac cycle. This allows flow quantification and visualization of blood flow patterns as well as the study of advanced hemodynamic parameters as kinetic energy and wall shear stress. 4D flow MRI-based study of flow has given insight into the altered hemodynamics in CHD particularly in bicuspid aortic valve disease and Fontan circulation. The aim of this review is to discuss the expanding clinical and research applications of 4D flow MRI in CHD as well its limitations.Key Points• Three-dimensional velocity encoding allows not only flow quantification but also the visualization of multidirectional flow patterns and the study of advanced hemodynamic parameters.• 4D flow MRI has added insight into the abnormal hemodynamics involved in congenital heart disease in particular in bicuspid aortic valve and Fontan circulation.• The main limitation of 4D flow MRI in congenital heart disease is the relatively long scan duration required for the complete coverage of the heart and great vessels with adequate spatiotemporal resolution.", "gemini": "Những tiến bộ trong chẩn đoán và quản lý bệnh tim bẩm sinh (CHD) đã dẫn đến sự gia tăng dân số bệnh nhân sống sót đến tuổi trưởng thành và cần theo dõi suốt đời. Định lượng lưu lượng là một thành phần trung tâm trong việc đánh giá bệnh nhân mắc CHD. Chụp cộng hưởng từ (MRI) dòng chảy 4D đã nổi lên như một công cụ cho phép nghiên cứu toàn diện về dòng chảy. Nó liên quan đến việc thu thập một thể tích ba chiều theo thời gian với mã hóa vận tốc theo cả ba hướng không gian dọc theo chu kỳ tim. Điều này cho phép định lượng lưu lượng và trực quan hóa các mẫu lưu lượng máu cũng như nghiên cứu các thông số huyết động học nâng cao như năng lượng động học và ứng suất cắt thành. Nghiên cứu lưu lượng dựa trên MRI dòng chảy 4D đã đưa ra cái nhìn sâu sắc về huyết động học thay đổi ở CHD, đặc biệt là ở bệnh van động mạch chủ hai lá và tuần hoàn Fontan. Mục đích của bài tổng quan này là thảo luận về các ứng dụng lâm sàng và nghiên cứu mở rộng của MRI dòng chảy 4D trong CHD cũng như những hạn chế của nó. Điểm chính • Mã hóa vận tốc ba chiều không chỉ cho phép định lượng lưu lượng mà còn trực quan hóa các mẫu lưu lượng đa hướng và nghiên cứu các thông số huyết động học nâng cao. • MRI dòng chảy 4D đã đưa ra cái nhìn sâu sắc về huyết động học bất thường liên quan đến bệnh tim bẩm sinh, đặc biệt là ở van động mạch chủ hai lá và tuần hoàn Fontan. • Hạn chế chính của MRI dòng chảy 4D trong bệnh tim bẩm sinh là thời gian quét tương đối dài cần thiết để bao phủ hoàn toàn tim và các mạch máu lớn với độ phân giải không gian và thời gian đầy đủ."} +{"idx": 1870591, "en": "An innovative ultrasensitive electrochemical aptamer-based sensor was developed for ochratoxin A (OTA) detection in cold brew coffee through revolutionary combination of nanofibers, electrochemical method, and aptamer technologies. The assembly of the aptasensor was based on the activation of silanized cellulose nanofibrous membranes as a supporting matrix for methylene blue (MB) redox probe-labeled aptamer tethering. Cellulose nanofibrous membranes were regenerated by deacetylating electrospun cellulose acetate nanofibrous membranes with deacetylation efficacy of 97%, followed by silanization of the nanofiber surfaces by using (3-aminopropyl)triethoxysilane (APTES). A replacement of conventionally casted membranes by the nanofibrous membranes increased the active surface area on the working electrode of a screen-printed three-electrode sensor by more than two times, consequently enhancing the fabricated aptasensor performance. The developed aptasensor demonstrated high sensitivity and specificity toward OTA in a range 0.002-2 ng mL-1, with a detection limit of 0.81 pg mL-1. Moreover, the assembled aptamer-based sensor successfully detected OTA in cold brew coffee samples without any pretreatment. The aptasensor exhibited good reusability and stability over long storage time. Graphical abstract.", "gemini": "Một cảm biến dựa trên aptme điện hóa siêu nhạy sáng tạo đã được phát triển để phát hiện ochratoxin A (OTA) trong cà phê pha lạnh thông qua sự kết hợp mang tính cách mạng của công nghệ nanofiber, phương pháp điện hóa và công nghệ aptme. Việc lắp ráp aptasensor dựa trên hoạt hóa màng nan sợi cellulose silan hóa làm ma trận hỗ trợ cho đầu dò oxy hóa khử xanh methylen (MB) gắn aptme được dán nhãn. Màng nan sợi cellulose được tái sinh bằng cách khử acetyl màng nan sợi axetat cellulose được kéo sợi bằng điện với hiệu quả khử acetyl là 97%, sau đó là silan hóa bề mặt nanofiber bằng cách sử dụng (3-aminopropyl) triethoxysilane (APTES). Việc thay thế màng đúc thông thường bằng màng nan sợi làm tăng diện tích bề mặt hoạt động trên điện cực làm việc của cảm biến ba điện cực in màn hình lên hơn hai lần, do đó tăng cường hiệu suất aptasensor chế tạo. Aptasensor được phát triển đã chứng minh độ nhạy cao và độ đặc hiệu đối với OTA trong phạm vi 0,002-2 ng mL-1, với giới hạn phát hiện là 0,81 pg mL-1. Hơn nữa, cảm biến dựa trên aptme lắp ráp đã phát hiện thành công OTA trong các mẫu cà phê pha lạnh mà không cần bất kỳ xử lý trước nào. Aptasensor thể hiện khả năng tái sử dụng và độ ổn định tốt trong thời gian bảo quản dài. Tóm tắt đồ họa."} +{"idx": 1870592, "en": "Maintenance of the main Golgi functions, glycosylation and sorting, is dependent on the unique Golgi pH microenvironment that is thought to be set by the balance between the rates of V-ATPase-mediated proton pumping and its leakage back to the cytoplasm via an unknown pathway. The concentration of other ions, such as chloride, potassium, calcium, magnesium, and manganese, is also important for Golgi homeostasis and dependent on the transport activity of other ion transporters present in the Golgi membranes. During the last decade, several new disorders have been identified that are caused by, or are associated with, dysregulated Golgi pH and ion homeostasis. Here, we will provide an updated overview on these disorders and the proteins involved. We will also discuss other disorders for which the molecular defects remain currently uncertain but which potentially involve proteins that regulate Golgi pH or ion homeostasis.", "gemini": "Việc duy trì các chức năng chính của Golgi, glycosyl hóa và phân loại, phụ thuộc vào vi môi trường pH Golgi độc đáo được cho là được thiết lập bởi sự cân bằng giữa tốc độ bơm proton do V-ATPase trung gian và rò rỉ trở lại tế bào chất thông qua một con đường chưa biết. Nồng độ của các ion khác, chẳng hạn như clorua, kali, canxi, magiê và mangan, cũng rất quan trọng đối với cân bằng nội môi Golgi và phụ thuộc vào hoạt động vận chuyển của các chất vận chuyển ion khác có trong màng Golgi. Trong thập kỷ qua, một số rối loạn mới đã được xác định là do pH Golgi và cân bằng ion bị rối loạn hoặc liên quan đến chúng. Ở đây, chúng tôi sẽ cung cấp một bản cập nhật tổng quan về các rối loạn này và các protein có liên quan. Chúng tôi cũng sẽ thảo luận về các rối loạn khác mà các khiếm khuyết về phân tử hiện vẫn chưa chắc chắn nhưng có khả năng liên quan đến các protein điều chỉnh pH Golgi hoặc cân bằng ion."} +{"idx": 1870593, "en": "Freshwater sources are limited and access to clean water is an acute challenge in recent decades. The sustainable water treatments methods are need of time and water desalination is one of the most interesting technology. Most desalination technologies are required high energy input while Microbial Desalination Cells (MDCs) represent a sustainable option that has added benefit of solving the ever-increasing wastewater treatment and management problem. MDCs are a customized type of Microbial Fuel Cells (MFCs) that depend on the electric potential generated by organic media to decrease salt concentration by electro-dialysis and give an unconventional way of clean water production. In this research, various experiments were conducted to examine the desalination ability of an indigenously designed experimental setup using domestic wastewater inoculated with sewage sludge under identical conditions. The electrochemical properties of the system, comprising the polarization curve and Electrochemical Impedance Spectroscopy (EIS), were examined along with the scope of chemical oxygen demand (COD) exclusion, to distinguish the cell behaviour. Furthermore, acidic water and Phosphate Buffer Solution (PBS) were tested as potential catholytes compared to the performance of the wastewater was gauged at various salt concentrations. The maximum salt removal efficiency was 31%, power density and current density were 32 mW-m-2 and 246 mA-m-2 respectively at a salt concentration of 35 g-L-1 that decreases with a decline in salt concentration. The maximum achieved power density and current density were 32 mW-m-2 and 246 mA-m-2 respectively. The applied method has huge potential to scaleup for large scale application in coastal regions.", "gemini": "Nguồn nước ngọt có hạn và việc tiếp cận nước sạch là một thách thức cấp tính trong những thập kỷ gần đây. Các phương pháp xử lý nước bền vững cần có thời gian và khử muối nước là một trong những công nghệ thú vị nhất. Hầu hết các công nghệ khử muối đều yêu cầu đầu vào năng lượng cao trong khi Tế bào khử muối vi sinh (MDC) đại diện cho một lựa chọn bền vững có lợi ích bổ sung là giải quyết vấn đề xử lý và quản lý nước thải ngày càng tăng. MDC là một loại Tế bào nhiên liệu vi sinh (MFC) tùy chỉnh phụ thuộc vào điện thế được tạo ra bởi môi trường hữu cơ để giảm nồng độ muối bằng điện phân và đưa ra một cách sản xuất nước sạch không theo quy ước. Trong nghiên cứu này, nhiều thí nghiệm đã được tiến hành để kiểm tra khả năng khử muối của một thiết bị thử nghiệm được thiết kế tại địa phương sử dụng nước thải sinh hoạt được cấy bùn thải trong điều kiện giống hệt nhau. Các tính chất điện hóa của hệ thống, bao gồm đường cong phân cực và Phổ trở kháng điện hóa (EIS), đã được kiểm tra cùng với phạm vi loại trừ nhu cầu oxy hóa học (COD), để phân biệt hành vi của tế bào. Hơn nữa, nước có tính axit và Dung dịch đệm phosphat (PBS) đã được thử nghiệm như các chất điện cực tiềm năng so với hiệu suất của nước thải được đo ở các nồng độ muối khác nhau. Hiệu suất loại bỏ muối tối đa là 31%, mật độ công suất và mật độ dòng điện lần lượt là 32 mW-m-2 và 246 mA-m-2 ở nồng độ muối 35 g-L-1 giảm khi nồng độ muối giảm. Mật độ công suất và mật độ dòng điện đạt được tối đa lần lượt là 32 mW-m-2 và 246 mA-m-2. Phương pháp áp dụng có tiềm năng rất lớn để mở rộng quy mô cho ứng dụng quy mô lớn ở các vùng ven biển."} +{"idx": 1870594, "en": "A series of haptens were rationally designed for producing monoclonal antibodies specific for EC and a simple fluorescence immunoassay platform was developed for the sensitive determination of EC based on alkaline phosphatase (ALP)-triggered Cu+ quenching of CdSe quantum dots (QDs). It was noted that Cd as a fluorescence substrate in CdSe QDs can be selectively substituted by Cu+ that resulted in a more significant fluorescence quenching in comparison with Cu2+. Meanwhile, because ALP catalyzed ascorbic acid phosphate and then assisted the transformation of Cu2+ to Cu+, the change in fluorescence intensity was found to be proportional to ALP concentration. After simple magnetic separation, the sensitivity and linear range of the established assay were improved approximately 53-fold and an order of magnitude, respectively, when compared with the conventional ELISA. The proposed platform was able to both amplify the signal and eliminate matrix interferences, making it a promising to determine EC as well as other contaminants in complex food matrix in a highly sensitive and simple manner. Graphical abstract.", "gemini": "Một loạt các haptens được thiết kế hợp lý để sản xuất các kháng thể đơn dòng đặc hiệu cho EC và một nền tảng miễn dịch huỳnh quang đơn giản đã được phát triển để xác định độ nhạy của EC dựa trên sự giải phóng Cu+ do phosphatase kiềm (ALP) kích hoạt của các chấm lượng tử CdSe (QD). Người ta thấy rằng Cd như một chất nền huỳnh quang trong CdSe QD có thể được thay thế một cách chọn lọc bằng Cu+, dẫn đến sự giải phóng huỳnh quang đáng kể hơn so với Cu2+. Trong khi đó, do ALP xúc tác axit ascorbic phosphate và sau đó hỗ trợ quá trình chuyển đổi Cu2+ thành Cu+, người ta thấy rằng sự thay đổi cường độ huỳnh quang tỷ lệ thuận với nồng độ ALP. Sau khi phân tách từ tính đơn giản, độ nhạy và phạm vi tuyến tính của xét nghiệm đã được thiết lập được cải thiện lần lượt khoảng 53 lần và một cấp độ lớn khi so sánh với ELISA thông thường. Nền tảng được đề xuất có khả năng khuếch đại tín hiệu và loại bỏ nhiễu ma trận, giúp xác định EC cũng như các chất gây ô nhiễm khác trong ma trận thực phẩm phức tạp một cách đơn giản và có độ nhạy cao. Tóm tắt đồ họa."} +{"idx": 1870595, "en": "CaLCuV-based VIGS effectively works in cabbage and contributes to efficient functional genomics research in Brassica crop species. Virus-induced gene silencing (VIGS), a posttranscriptional gene silencing method, is an effective technique for analysing the functions of genes in plants. However, no VIGS vectors have been available for Brassica oleracea until now. Here, tobacco rattle virus (TRV), pTYs and cabbage leaf curl virus (CaLCuV) gene-silencing vectors (PCVA/PCVB) were chosen to improve the VIGS system in cabbage using the phytoene desaturase (PDS) gene as an efficient visual indicator of VIGS. We successfully silenced the expression of PDS and observed photobleaching phenomena in cabbage in response to pTYs and CaLCuV, with the latter being more easy to operate and less expensive. The parameters potentially affecting the silencing efficiency of VIGS by CaLCuV in cabbage, including the targeting fragment strategy, inoculation method and incubation temperature, were then compared. The optimized CaLCuV-based VIGS system involves the following: an approximately 500 bp insert sequence, an Agrobacterium OD600 of 1.0, use of the vacuum osmosis method applied at the bud stage, and an incubation temperature of 22 °C. Using these parameters, we achieved a stable silencing efficiency of 65%. To further test the effectiveness of the system, we selected the Mg-chelatase H subunit (ChlH) gene in cabbage and knocked down its expression, and we observed yellow leaves, as expected. We successfully applied the CaLCuV-based VIGS system to two other representative Brassica crop species, B. rapa and B. nigra, and thus expanded the application scope of this system. Our VIGS system described here will contribute to efficient functional genomics research in Brassica crop species.", "gemini": "VIGS dựa trên CaLCuV hoạt động hiệu quả ở bắp cải và góp phần vào nghiên cứu về hệ gen chức năng hiệu quả ở các loài cây họ cải. Tắt gen do vi-rút gây ra (VIGS), một phương pháp tắt gen sau phiên mã, là một kỹ thuật hiệu quả để phân tích chức năng của gen ở thực vật. Tuy nhiên, cho đến nay vẫn chưa có vectơ VIGS nào có sẵn cho Brassica oleracea. Ở đây, vectơ gây im lặng gen của vi-rút gây loang lá thuốc lá (TRV), pTYs và vi-rút làm lá bắp cải cong (CaLCuV) (PCVA/PCVB) đã được chọn để cải thiện hệ thống VIGS ở bắp cải bằng cách sử dụng gen phytoene desaturase (PDS) làm chỉ thị trực quan hiệu quả của VIGS. Chúng tôi đã thành công trong việc làm im lặng biểu hiện của PDS và quan sát thấy hiện tượng mất màu ở bắp cải đáp ứng với pTYs và CaLCuV, trong đó CaLCuV dễ vận hành hơn và ít tốn kém hơn. Các thông số có khả năng ảnh hưởng đến hiệu quả im lặng của VIGS bởi CaLCuV ở bắp cải, bao gồm chiến lược phân đoạn nhắm mục tiêu, phương pháp tiêm chủng và nhiệt độ ủ, sau đó được so sánh. Hệ thống VIGS dựa trên CaLCuV được tối ưu hóa bao gồm: trình tự chèn khoảng 500 bp, Agrobacterium OD600 là 1,0, sử dụng phương pháp thẩm thấu chân không áp dụng ở giai đoạn chồi và nhiệt độ ủ là 22 °C. Sử dụng các thông số này, chúng tôi đạt được hiệu quả im lặng ổn định là 65%. Để kiểm tra thêm hiệu quả của hệ thống, chúng tôi đã chọn gen tiểu đơn vị H của Mg-chelatase (ChlH) ở bắp cải và loại bỏ biểu hiện của nó, và chúng tôi quan sát thấy lá có màu vàng như mong đợi. Chúng tôi đã thành công trong việc áp dụng hệ thống VIGS dựa trên CaLCuV cho hai loài cây họ cải đại diện khác, B. rapa và B. nigra, và do đó mở rộng phạm vi ứng dụng của hệ thống này. Hệ thống VIGS của chúng tôi được mô tả ở đây sẽ góp phần vào nghiên cứu về hệ gen chức năng hiệu quả ở các loài cây họ cải."} +{"idx": 1870596, "en": "During spring 2018, we captured 101 spur-thighed tortoises, Testudo graeca, from seven localities in central Morocco. All tortoises were examined for the presence of blood parasites Hemolivia mauritanica and Hyalomma aegyptium ticks, the known vectors. We looked for H. mauritanica infection by examination of blood smears and by genetic analysis with PCR using extractions from both tortoises and ticks. On all tortoises collected, 71.29% were infested with at least one tick, with a mean infestation intensity of 7.12 ticks/tortoise and maximum of 15.55 ticks/tortoises in Had Draa locality (Essaouira region). Although the definitive host is present and abundant in all tortoise populations, all blood samples were Hemolivia-negative. Our results support and confirm the finding of studies previously conducted in other populations of Morocco and indicate that H. mauritanica has a narrower distribution range than its tick vector.", "gemini": "Vào mùa xuân năm 2018, chúng tôi đã bắt được 101 con rùa đùi gai, Testudo graeca, từ bảy địa phương ở miền trung Ma-rốc. Tất cả rùa đã được kiểm tra sự hiện diện của ký sinh trùng máu Hemolivia mauritanica và ve Hyalomma aegyptium, những vật trung gian đã biết. Chúng tôi tìm kiếm nhiễm trùng H. mauritanica bằng cách kiểm tra máu phết và bằng phân tích di truyền với PCR sử dụng dịch chiết từ cả rùa và ve. Trên tất cả những con rùa được thu thập, 71,29% bị nhiễm ít nhất một con ve, với cường độ nhiễm trung bình là 7,12 con ve/rùa và tối đa là 15,55 con ve/rùa ở địa phương Had Draa (khu vực Essaouira). Mặc dù vật chủ xác định có mặt và rất nhiều trong tất cả các quần thể rùa, nhưng tất cả các mẫu máu đều âm tính với Hemolivia. Kết quả của chúng tôi hỗ trợ và xác nhận phát hiện của các nghiên cứu trước đây được tiến hành ở các quần thể khác của Ma-rốc và chỉ ra rằng H. mauritanica có phạm vi phân bố hẹp hơn vật trung gian truyền ve của nó."} +{"idx": 1870597, "en": "The podovirus BPP-1 is currently the only member of the Podovirus genus Rauchvirus. Here, we describe three new Caulobacter bacteriophages (Jess A, SR18, and RW) that show genetic similarity to BPP-1 but have many different genetic and structural features that differentiate them from BPP-1. Jess A and SR18 are closely related to each other and should be considered two members of a new species. They share a similar gene order with BPP-1. However, they do not appear to form lysogens or have the tropism switching mechanism that has been described for BPP-1. Bacteriophage RW also exhibits some homology to BPP-1. However, it is quite different from the other three phages, and we propose that it should be considered a representative of a third species of the genus Rauchvirus. Taken together, the differences among these four members of the genus Rauchvirus indicate that this divergent genus has a long evolutionary history and that there are many more rauchviruses waiting to be discovered.", "gemini": "Podovirus BPP-1 hiện là thành viên duy nhất của chi Podovirus Rauchvirus. Tại đây, chúng tôi mô tả ba loại thực khuẩn Caulobacter mới (Jess A, SR18 và RW) cho thấy sự tương đồng về mặt di truyền với BPP-1 nhưng có nhiều đặc điểm di truyền và cấu trúc khác biệt để phân biệt chúng với BPP-1. Jess A và SR18 có quan hệ họ hàng gần với nhau và nên được coi là hai thành viên của một loài mới. Chúng có chung một trật tự gen tương tự với BPP-1. Tuy nhiên, chúng dường như không tạo thành lysogen hoặc có cơ chế chuyển đổi hướng động vật chủ đã được mô tả đối với BPP-1. Thực khuẩn RW cũng biểu hiện một số tính đồng đẳng với BPP-1. Tuy nhiên, nó khá khác so với ba loại thực khuẩn khác và chúng tôi đề xuất rằng nó nên được coi là đại diện của loài thứ ba của chi Rauchvirus. Xét về tổng thể, sự khác biệt giữa bốn thành viên này của chi Rauchvirus chỉ ra rằng chi phân kỳ này có lịch sử tiến hóa lâu dài và còn nhiều loại rauchvirus khác đang chờ được khám phá."} +{"idx": 1870598, "en": "By replacing hydrogen atoms in thymine molecules with nitro groups, a series of new high-energy-density molecules are designed. To explore the thermal stability, the heats of formation (HOF) are calculated at the B3PW91-D3/6-311++G(2df,2p) level. The bond dissociation energy and the bond order are also calculated to predict the kinetic stability at the same level. Based on our calculations, excellent stability is confirmed for title molecules. To confirm the possibility of application as high-energy-density compounds, the molecular density (ρ), explosive heats (Q), detonation velocity (D), detonation pressure (P), free space per molecule in crystal lattice (ΔV), and characteristic drop height (H50) are calculated. On the consideration of the stability and the detonation characters, E1 is confirmed as the candidates of high-energy-density compounds.", "gemini": "Bằng cách thay thế các nguyên tử hydro trong các phân tử thymine bằng các nhóm nitro, một loạt các phân tử mật độ năng lượng cao mới được thiết kế. Để khám phá độ ổn định nhiệt, nhiệt hình thành (HOF) được tính toán ở mức B3PW91-D3/6-311++G(2df,2p). Năng lượng phân ly liên kết và bậc liên kết cũng được tính toán để dự đoán độ ổn định động học ở cùng mức. Dựa trên các tính toán của chúng tôi, độ ổn định tuyệt vời được xác nhận đối với các phân tử tiêu đề. Để xác nhận khả năng ứng dụng làm hợp chất mật độ năng lượng cao, mật độ phân tử (ρ), nhiệt nổ (Q), vận tốc nổ (D), áp suất nổ (P), không gian tự do cho mỗi phân tử trong mạng tinh thể (ΔV) và độ rơi đặc trưng (H50) được tính toán. Khi xem xét độ ổn định và các đặc tính nổ, E1 được xác nhận là ứng cử viên của các hợp chất mật độ năng lượng cao."} +{"idx": 1870599, "en": "PURPOSE OF REVIEW The alarming number of confirmed COVID-19 cases put a strain on the healthcare systems, which had to reallocate human and technical resources to respond to the emergency. Many urologists became integrated into multidisciplinary teams, dealing with this respiratory illness and its unknown management. It aims to summarize the epidemiological, clinical, diagnostical, and therapeutical characteristics of COVID-19, from a practical perspective, to ease COVID-19 management to non-physician staff. RECENT FINDINGS We performed a narrative review of the literature regarding COVID-19, updated to May 8th, 2020, at PubMed and COVID resource platforms of the main scientific editorials. COVID-19, characterized by fever, myalgias, dyspnea, and dry cough, varies widely from asymptomatic infection to death. Arrhythmias and thrombotic events are prevalent. Lymphopenia and inflammatory reactant elevation on laboratory, as well as bilateral and peripheral ground-glass opacities or consolidations on X-Ray, are usually found in its assessment. Little is known about SARS-CoV-2 immunology. To date, no therapy has demonstrated efficacy in COVID-19. Of-level or compassionate-use therapies are prescribed in the context of clinical trials. We should become familiar with specific adverse events and pharmacological interactions. The COVID-19 pandemic has paralyzed the urological activity, and its long-term consequences are unpredictable. Despite not being used to deal with respiratory diseases, the urologists become easily qualified to manage COVID-19 by following protocols and being integrated into multidisciplinary teams, helping to overcome the pandemic.", "gemini": "MỤC ĐÍCH CỦA ĐÁNH GIÁ Số ca mắc COVID-19 được xác nhận báo động đã gây căng thẳng cho các hệ thống chăm sóc sức khỏe, vốn phải phân bổ lại nguồn lực về con người và kỹ thuật để ứng phó với tình trạng khẩn cấp. Nhiều bác sĩ tiết niệu đã được tích hợp vào các nhóm đa chuyên khoa, đối phó với căn bệnh về đường hô hấp này và cách xử trí chưa được biết đến của nó. Mục đích là để tóm tắt các đặc điểm dịch tễ học, lâm sàng, chẩn đoán và điều trị của COVID-19, từ góc độ thực tế, để dễ dàng quản lý COVID-19 cho đội ngũ nhân viên không phải bác sĩ. PHÁT HIỆN GẦN ĐÂY Chúng tôi đã thực hiện một đánh giá tường thuật về các tài liệu liên quan đến COVID-19, được cập nhật đến ngày 8 tháng 5 năm 2020, tại PubMed và các nền tảng tài nguyên COVID của các biên tập viên khoa học chính. COVID-19, được đặc trưng bởi sốt, đau nhức cơ, khó thở và ho khan, thay đổi rất nhiều từ nhiễm trùng không có triệu chứng đến tử vong. Rối loạn nhịp tim và các biến cố huyết khối rất phổ biến. Giảm bạch cầu và tăng phản ứng viêm trong xét nghiệm, cũng như tình trạng mờ thủy tinh hoặc hợp nhất ở ngoại vi và hai bên trên X-quang, thường được tìm thấy trong đánh giá của bệnh. Người ta biết rất ít về miễn dịch học của SARS-CoV-2. Cho đến nay, chưa có liệu pháp nào chứng minh được hiệu quả đối với COVID-19. Các liệu pháp dùng ngoài cấp độ hoặc sử dụng từ bi được kê đơn trong bối cảnh thử nghiệm lâm sàng. Chúng ta nên làm quen với các tác dụng phụ cụ thể và tương tác dược lý. Đại dịch COVID-19 đã làm tê liệt hoạt động tiết niệu và hậu quả lâu dài của nó là không thể đoán trước. Mặc dù không quen đối phó với các bệnh về đường hô hấp, các bác sĩ tiết niệu dễ dàng đủ điều kiện để quản lý COVID-19 bằng cách tuân theo các giao thức và được tích hợp vào các nhóm đa chuyên khoa, giúp vượt qua đại dịch."} +{"idx": 1870600, "en": "Paleontology has long relied on assumptions about the genetic and developmental influences on skeletal variation. The last few decades of developmental genetics have elucidated the genetic pathways involved in making teeth and patterning the dentition. Quantitative genetic analyses have refined this genotype:phenotype map even more, especially for primates. We now have the ability to define dental traits with a fair degree of fidelity to the underlying genetic architecture; for example, the molar module component (MMC) and the premolar-molar module (PMM) that have been defined through quantitative genetic analyses. We leverage an extensive dataset of extant and extinct hominoid dental variation to explore how these two genetically patterned phenotypes have evolved through time. We assess MMC and PMM to test the hypothesis that these two traits reveal a more biologically informed taxonomy at the genus and species levels than do more traditional measurements. Our results indicate that MMC values for hominids fall into two categories and that Homo is derived compared with earlier taxa. We find a more variable, species-level pattern for PMM. These results, in combination with previous research, demonstrate that MMC reflects the phenotypic output of a more evolutionarily stable, or phylogenetically congruent, genetic mechanism, and PMM is a reflection of a more evolutionarily labile mechanism. These results suggest that the human lineage since the split with chimpanzees may not represent as much genus-level variation as has been inferred from traits whose etiologies are not understood.", "gemini": "Cổ sinh vật học từ lâu đã dựa trên các giả định về ảnh hưởng của di truyền và phát triển lên sự biến đổi của bộ xương. Vài thập kỷ gần đây của di truyền học phát triển đã làm sáng tỏ các con đường di truyền liên quan đến việc tạo ra răng và tạo mẫu cho bộ răng. Các phân tích di truyền định lượng đã tinh chỉnh bản đồ kiểu gen: kiểu hình này thậm chí còn nhiều hơn, đặc biệt là đối với loài linh trưởng. Bây giờ chúng ta có khả năng xác định các đặc điểm của răng với mức độ trung thành khá cao với cấu trúc di truyền cơ bản; ví dụ, thành phần mô răng hàm (MMC) và mô răng tiền hàm (PMM) đã được xác định thông qua các phân tích di truyền định lượng. Chúng tôi sử dụng một bộ dữ liệu rộng lớn về sự biến đổi răng của vượn người hiện đại và đã tuyệt chủng để khám phá cách hai kiểu hình có kiểu gen này tiến hóa theo thời gian như thế nào. Chúng tôi đánh giá MMC và PMM để kiểm tra giả thuyết rằng hai đặc điểm này tiết lộ một phân loại được thông tin sinh học nhiều hơn ở cấp độ chi và loài so với các phép đo truyền thống hơn. Kết quả của chúng tôi chỉ ra rằng các giá trị MMC đối với họ người chia thành hai loại và Homo có nguồn gốc so với các loài trước đó. Chúng tôi tìm thấy một kiểu mẫu biến đổi hơn ở cấp độ loài đối với PMM. Những kết quả này, kết hợp với các nghiên cứu trước đây, chứng minh rằng MMC phản ánh kết quả kiểu hình của một cơ chế di truyền ổn định hơn về mặt tiến hóa hoặc phù hợp về mặt phát sinh loài, và PMM là sự phản ánh của một cơ chế không ổn định hơn về mặt tiến hóa. Những kết quả này cho thấy rằng dòng dõi của con người kể từ khi tách khỏi tinh tinh có thể không đại diện cho nhiều biến thể ở cấp độ chi như đã suy ra từ các đặc điểm mà nguyên nhân của chúng không được hiểu rõ."} +{"idx": 1870601, "en": "BACKGROUND Formamides are common motifs of biologically-active compounds (e.g. formylated peptides) and are frequently employed as intermediates to yield a number of other functional groups. A rapid, simple and reliable route to [carbonyl-11C]formamides would enable access to this important class of compounds as in vivo PET imaging agents. RESULTS A novel radiolabelling strategy for the synthesis of carbon-11 radiolabelled formamides ([11C]formamides) is presented. The reaction proceeded with the conversion of a primary amine to the corresponding [11C]isocyanate using cyclotron-produced [11C]CO2, a phosphazene base (2-tert-butylimino-2-diethylamino-1,3-dimethylperhydro-1,3,2-diazaphosphorine, BEMP) and phosphoryl chloride (POCl3). The [11C]isocyanate was subsequently reduced to [11C]formamide using sodium borohydride (NaBH4). [11C]Benzyl formamide was obtained with a radiochemical yield (RCY) of 80% in 15 min from end of cyclotron target bombardment and with an activity yield of 12%. This novel method was applied to the radiolabeling of aromatic and aliphatic formamides and the chemotactic amino acid [11C]formyl methionine (RCY = 48%). CONCLUSIONS This study demonstrates the feasibility of 11C-formylation of primary amines with the primary synthon [11C]CO2. The reactivity is proportional to the nucleophilicity of the precursor amine. This novel method can be used for the production of biomolecules containing a radiolabelled formyl group.", "gemini": "HOÀN CẢNH Formamide là các họa tiết phổ biến của các hợp chất có hoạt tính sinh học (ví dụ như các peptit được formyl hóa) và thường được sử dụng làm chất trung gian để tạo ra một số nhóm chức năng khác. Một con đường nhanh chóng, đơn giản và đáng tin cậy đến [cacbonyl-11C]formamide sẽ cho phép tiếp cận nhóm hợp chất quan trọng này như các tác nhân tạo ảnh PET trong cơ thể. KẾT QUẢ Một chiến lược đánh dấu phóng xạ mới để tổng hợp các formamide được đánh dấu phóng xạ bằng cacbon-11 ([11C]formamide) được trình bày. Phản ứng diễn ra với quá trình chuyển đổi amin bậc một thành [11C]isocyanate tương ứng bằng cách sử dụng [11C]CO2 do máy gia tốc cyclotron tạo ra, một bazơ phosphazene (2-tert-butylimino-2-diethylamino-1,3-dimethylperhydro-1,3,2-diazaphosphorine, BEMP) và phosphoryl clorua (POCl3). [11C]isocyanate sau đó được khử thành [11C]formamide bằng natri borohydride (NaBH4). [11C]Benzyl formamide thu được với hiệu suất phản ứng hóa học (RCY) là 80% trong 15 phút kể từ khi kết thúc quá trình bắn phá mục tiêu cyclotron và với hiệu suất hoạt động là 12%. Phương pháp mới này được áp dụng để đánh dấu phóng xạ các formamide thơm và aliphatic và axit amin hướng hóa học [11C]formyl methionine (RCY = 48%). KẾT LUẬN Nghiên cứu này chứng minh tính khả thi của quá trình formyl hóa 11C của các amin bậc một với [11C]CO2 tổng hợp chính. Độ phản ứng tỷ lệ thuận với tính ái nucleophin của amin tiền chất. Phương pháp mới này có thể được sử dụng để sản xuất các phân tử sinh học có chứa nhóm formyl được đánh dấu phóng xạ."} +{"idx": 1870602, "en": "Glioblastoma (GBM) is one of the most malignant primary brain tumors. This neoplasm is the hardest to treat and has a bad prognosis. Because of the characteristics of genetic heterogeneity and frequent recurrence, a successful cure for the disease is unlikely. Increasing evidence has revealed that the GBM stem cell-like cells (GSCs) and microenvironment are key elements in GBM recurrence and treatment failure. To better understand the mechanisms underlying this disease and to develop more effective therapeutic strategies for treatment, suitable approaches, techniques, and model systems closely mimicking real GBM conditions are required. Microfluidic devices, a model system mimicking the in vivo brain microenvironment, provide a very useful tool to analyze GBM cell behavior, their correlation with tumor malignancy, and the efficacy of multiple drug treatment. This paper reviews the applications of microfluidic devices in GBM research and summarizes progress and perspectives in this field.", "gemini": "U nguyên bào thần kinh đệm (GBM) là một trong những khối u não nguyên phát ác tính nhất. Khối u này khó điều trị nhất và có tiên lượng xấu. Do đặc điểm di truyền không đồng nhất và tái phát thường xuyên, nên không có khả năng chữa khỏi bệnh thành công. Ngày càng có nhiều bằng chứng cho thấy các tế bào giống tế bào gốc GBM (GSC) và môi trường vi mô là những yếu tố chính trong quá trình tái phát GBM và thất bại trong điều trị. Để hiểu rõ hơn về các cơ chế gây ra căn bệnh này và phát triển các chiến lược điều trị hiệu quả hơn, cần có các phương pháp tiếp cận, kỹ thuật và hệ thống mô hình phù hợp bắt chước chặt chẽ các tình trạng GBM thực tế. Các thiết bị vi lưu, một hệ thống mô hình bắt chước môi trường vi mô của não trong cơ thể, cung cấp một công cụ rất hữu ích để phân tích hành vi của tế bào GBM, mối tương quan của chúng với tính ác tính của khối u và hiệu quả của nhiều phương pháp điều trị bằng thuốc. Bài báo này xem xét các ứng dụng của các thiết bị vi lưu trong nghiên cứu GBM và tóm tắt tiến độ và triển vọng trong lĩnh vực này."} +{"idx": 1870603, "en": "INTRODUCTION Depression symptoms, frequently diagnosed in older patients with cancer, impacts on oncological treatment feasibility. The Francophone Society of Geriatric Oncology (SOFOG) has initiated a systematic review on depression treatment in older patients with cancer, to advocate guidelines. DATA SOURCES Medline via PubMed, Embase, CENTRAL. METHODS We included randomized and non-randomized controlled trials, reviews and meta-analysis, retrospective and prospective cohort studies, qualitative studies, and guidelines published between January 2013 and December 2018 that involved depression with cancer in which the entire sample or a sub-group aged 65 and above. Efficacy and tolerance of depression treatment were examined, as a primary or secondary outcome, among articles published in French or English. RESULTS Of 3171 references, only seven studies met our eligibility criteria. This systematic review reveals a lack of evidence-based knowledge in this field, preventing from making any recommendations on drug and non-drug therapies. It has highlighted the need for multidisciplinary collaboration with the French and Francophone Society of Psycho-Oncology. CONCLUSION In clinical practice, we advise health professionals to use the screening process not as a result but rather as an opportunity to engage with the patient and also to question the need for antidepressants and non-drug therapies.", "gemini": "GIỚI THIỆU Các triệu chứng trầm cảm, thường được chẩn đoán ở những bệnh nhân ung thư lớn tuổi, ảnh hưởng đến tính khả thi của việc điều trị ung thư. Hội Ung thư Lão khoa Pháp ngữ (SOFOG) đã khởi xướng một đánh giá có hệ thống về việc điều trị trầm cảm ở những bệnh nhân ung thư lớn tuổi, để đưa ra các hướng dẫn. CÁC NGUỒN DỮ LIỆU Medline qua PubMed, Embase, CENTRAL. PHƯƠNG PHÁP Chúng tôi đã đưa vào các thử nghiệm có đối chứng ngẫu nhiên và không ngẫu nhiên, các đánh giá và phân tích tổng hợp, các nghiên cứu đoàn hệ hồi cứu và triển vọng, các nghiên cứu định tính và các hướng dẫn được công bố từ tháng 1 năm 2013 đến tháng 12 năm 2018 liên quan đến trầm cảm với ung thư trong đó toàn bộ mẫu hoặc một nhóm phụ từ 65 tuổi trở lên. Hiệu quả và khả năng dung nạp của việc điều trị trầm cảm đã được kiểm tra, như một kết quả chính hoặc thứ cấp, trong số các bài báo được công bố bằng tiếng Pháp hoặc tiếng Anh. KẾT QUẢ Trong số 3171 tài liệu tham khảo, chỉ có bảy nghiên cứu đáp ứng các tiêu chí đủ điều kiện của chúng tôi. Đánh giá có hệ thống này cho thấy thiếu kiến thức dựa trên bằng chứng trong lĩnh vực này, ngăn cản việc đưa ra bất kỳ khuyến nghị nào về liệu pháp dùng thuốc và không dùng thuốc. Nó đã nêu bật nhu cầu hợp tác đa ngành với Hội Tâm lý ung thư Pháp và Pháp ngữ. KẾT LUẬN Trong thực hành lâm sàng, chúng tôi khuyên các chuyên gia y tế nên sử dụng quy trình sàng lọc không phải như một kết quả mà đúng hơn là một cơ hội để giao tiếp với bệnh nhân và cũng để đặt câu hỏi về nhu cầu sử dụng thuốc chống trầm cảm và liệu pháp không dùng thuốc."} +{"idx": 1870604, "en": "PURPOSE Chemotherapy-induced taste and smell alterations may have a negative impact on the quality of life and nutritional status. A prominent issue when dealing with taste and smell alterations and their consequences on food behavior and well-being lies in the variation arising from individual differences in chemosensory perceptions. The main aim of this study was to examine the effect of individuals' variation in the severity of taste and smell alterations relative to the stage of chemotherapy on self-reported food behavior and food perception. METHODS Eighty-nine cancer patients completed a questionnaire subdivided into two parts: a chemosensory part that allowed classification of patients in three groups (\"no alterations,\" \"moderate alterations,\" and \"severe alterations\") and a food behavior part. RESULTS The results highlighted a negative impact of chemosensory alterations on food perception. Compared with patients without taste and smell alterations, patients with severe chemosensory alterations reported significantly more frequent food perception problems, including modification of the perceived taste of food, finding bad taste in all food, and being unable to perceive food taste. Whereas 72% of patients with severe alterations were in late stage, only 37% of patients were in late stage in the no alterations group, indicating an effect of the treatment stage on taste and smell alterations. CONCLUSION Our results underlie the importance of providing specific attention to the severity of chemotherapy-induced taste and smell alterations and considering the individual differences among patients for a better nutritional management.", "gemini": "MỤC ĐÍCH Những thay đổi về vị giác và khứu giác do hóa trị có thể ảnh hưởng tiêu cực đến chất lượng cuộc sống và tình trạng dinh dưỡng. Một vấn đề nổi bật khi giải quyết những thay đổi về vị giác và khứu giác cùng hậu quả của chúng đối với hành vi ăn uống và sức khỏe nằm ở sự khác biệt phát sinh từ những khác biệt của từng cá nhân trong nhận thức về cảm giác hóa học. Mục đích chính của nghiên cứu này là kiểm tra tác động của sự khác biệt của từng cá nhân về mức độ nghiêm trọng của những thay đổi về vị giác và khứu giác so với giai đoạn hóa trị đối với hành vi ăn uống và nhận thức về thức ăn tự báo cáo. PHƯƠNG PHÁP Tám mươi chín bệnh nhân ung thư đã hoàn thành bảng câu hỏi được chia thành hai phần: một phần về cảm giác hóa học cho phép phân loại bệnh nhân thành ba nhóm (\"không thay đổi\", \"thay đổi vừa phải\" và \"thay đổi nghiêm trọng\") và một phần về hành vi ăn uống. KẾT QUẢ Kết quả làm nổi bật tác động tiêu cực của những thay đổi về cảm giác hóa học đối với nhận thức về thức ăn. So với những bệnh nhân không có thay đổi về vị giác và khứu giác, những bệnh nhân có thay đổi về cảm giác hóa học nghiêm trọng báo cáo thường xuyên gặp vấn đề về nhận thức về thức ăn nhiều hơn đáng kể, bao gồm thay đổi về vị giác nhận thức của thức ăn, thấy tất cả thức ăn đều có vị khó chịu và không cảm nhận được vị của thức ăn. Trong khi 72% bệnh nhân có thay đổi nghiêm trọng ở giai đoạn cuối, thì chỉ có 37% bệnh nhân ở giai đoạn cuối trong nhóm không có thay đổi, cho thấy tác động của giai đoạn điều trị đến những thay đổi về vị giác và khứu giác. KẾT LUẬN Kết quả của chúng tôi nhấn mạnh tầm quan trọng của việc chú ý cụ thể đến mức độ nghiêm trọng của những thay đổi về vị giác và khứu giác do hóa trị gây ra và xem xét những khác biệt của từng cá nhân giữa những bệnh nhân để có chế độ quản lý dinh dưỡng tốt hơn."} +{"idx": 1870605, "en": "The noninvasive early detection of biomarkers for Alzheimer's disease (AD) is essential for the development of specific treatment strategies. This paper proposes an advanced method for fabricating highly ordered and flexible nanopillar-based electrochemical biosensors by the combination of soft/photolithography and metal evaporation. The nanopillar array (NPA) exhibits high surface area containing 1500 nm height and 500 nm diameter with 3:1 ratio. In regard with physical properties of polyurethane (PU) substrate, the developed NPA is sustainable and durable to external pressure such as bending and twisting. To manipulate the NPA surface to biocompatible, the gold was uniformly deposited on the PU substrate. The thiol chemistry which is stably modified on the gold surface as a form of self-assembled monolayer was employed for fabricating the NPA as a biocompatible chip by covalently immobilize the antibodies. The proposed nanopillar-based immunoelectrochemical biosensor exhibited good and stable electrochemical performance in β-amyloid (Aβ) detection. Moreover, we successfully confirmed the performance of the as-developed sensor using the artificial injection of Aβ in human tear, with sensitivity of 0.14 ng/mL and high reproducibility (as a standard deviation below 10%). Our findings show that the developed nanopillar-based sensor exhibits reliable electrochemical characteristics and prove its potential for application as a biosensor platform for testing at the point of care.", "gemini": "Việc phát hiện sớm không xâm lấn các dấu ấn sinh học của bệnh Alzheimer (AD) là rất cần thiết cho việc phát triển các chiến lược điều trị cụ thể. Bài báo này đề xuất một phương pháp tiên tiến để chế tạo các cảm biến sinh học điện hóa dựa trên nanopillar có độ linh hoạt và sắp xếp cao bằng cách kết hợp quang khắc mềm/ánh sáng và bay hơi kim loại. Mảng nanopillar (NPA) thể hiện diện tích bề mặt cao chứa chiều cao 1500 nm và đường kính 500 nm với tỷ lệ 3:1. Về các tính chất vật lý của chất nền polyurethane (PU), NPA được phát triển bền vững và bền bỉ với áp lực bên ngoài như uốn cong và xoắn. Để xử lý bề mặt NPA thành tương thích sinh học, vàng được lắng đọng đồng đều trên chất nền PU. Hóa học thiol được sửa đổi ổn định trên bề mặt vàng dưới dạng lớp đơn phân tử tự lắp ráp đã được sử dụng để chế tạo NPA thành chip tương thích sinh học bằng cách cố định cộng hóa trị các kháng thể. Cảm biến sinh học điện hóa miễn dịch dựa trên nanopillar được đề xuất thể hiện hiệu suất điện hóa tốt và ổn định trong việc phát hiện β-amyloid (Aβ). Hơn nữa, chúng tôi đã xác nhận thành công hiệu suất của cảm biến được phát triển khi sử dụng tiêm nhân tạo Aβ vào nước mắt người, với độ nhạy 0,14 ng/mL và khả năng tái tạo cao (độ lệch chuẩn dưới 10%). Những phát hiện của chúng tôi cho thấy cảm biến dựa trên nanopillar được phát triển thể hiện các đặc tính điện hóa đáng tin cậy và chứng minh tiềm năng của nó để ứng dụng làm nền tảng cảm biến sinh học để thử nghiệm tại nơi chăm sóc."} +{"idx": 1870606, "en": "Play is often used in interventions to improve social outcomes for children with autism spectrum disorders (ASD). Play is a primary occupation of childhood and, therefore, an important outcome of intervention. The Ultimate Guide to Play, Language and Friendship (PLF) is a peer-mediated intervention for 6-11-year-old children with ASD. A total of 68 dyads were randomized to either a 10-week treatment first or waitlist control group. Results revealed a significant moderate intervention effect from pre- to post-intervention, which was maintained to the 3-month follow-up clinic session and generalized to the home environment. The findings support that the PLF intervention can be used to positively improve play in 6-11-year-old children with ASD.Australian New Zealand Clinical Trials Registry, https://www.anzctr.org.au/ (ACTRN12615000008527; Universal Trial Number: U1111-1165-2708).", "gemini": "Trò chơi thường được sử dụng trong các biện pháp can thiệp để cải thiện các kết quả xã hội cho trẻ em mắc chứng rối loạn phổ tự kỷ (ASD). Trò chơi là hoạt động chính của thời thơ ấu và do đó, là một kết quả quan trọng của sự can thiệp. Hướng dẫn tối ưu về trò chơi, ngôn ngữ và tình bạn (PLF) là một biện pháp can thiệp do bạn bè làm trung gian dành cho trẻ em từ 6-11 tuổi mắc chứng ASD. Tổng cộng 68 cặp đôi được phân ngẫu nhiên vào nhóm điều trị đầu tiên trong 10 tuần hoặc nhóm đối chứng danh sách chờ. Kết quả cho thấy tác dụng can thiệp vừa phải đáng kể từ trước can thiệp đến sau can thiệp, được duy trì đến buổi khám theo dõi tại phòng khám sau 3 tháng và được tổng quát hóa cho môi trường gia đình. Những phát hiện này ủng hộ rằng can thiệp PLF có thể được sử dụng để cải thiện tích cực trò chơi ở trẻ em từ 6-11 tuổi mắc chứng ASD. Sổ đăng ký thử nghiệm lâm sàng Úc New Zealand, https://www.anzctr.org.au/ (ACTRN12615000008527; Số thử nghiệm chung: U1111-1165-2708)."} +{"idx": 1870607, "en": "The diversity of modern society is often not represented in the medical workforce. This might be partly due to selection practices. We need to better understand decision-making processes by selection committees in order to improve selection procedures with regard to diversity. This paper reports on a qualitative study with a socio-constructivist perspective conducted in 2015 that explored how residency selection decision-making occurred within four specialties in two regions in the Netherlands. Data included transcripts of the decision-making meetings and of one-on-one interviews with committee members before and after the group decision-making meetings. Candidates struggled to portray themselves favorably as they had to balance playing by the rules and being authentic; between fitting in and standing out. Although admissions committees had a welcoming stance to diversity, their practices were unintentionally preventing them from hiring underrepresented minority (URM) candidates. While negotiating admissions is difficult for all candidates, it is presumably even more complicated for URM candidates. This seems to be having a negative influence on attaining workforce diversity. Current beliefs, which make committees mistakenly feel they are acting fairly, might actually justify biased practices. Awareness of the role of committee members in these processes is an essential first step.", "gemini": "Sự đa dạng của xã hội hiện đại thường không được thể hiện trong lực lượng lao động y tế. Điều này có thể một phần là do các hoạt động tuyển chọn. Chúng ta cần hiểu rõ hơn về các quy trình ra quyết định của các ủy ban tuyển chọn để cải thiện các quy trình tuyển chọn liên quan đến sự đa dạng. Bài báo này báo cáo về một nghiên cứu định tính với quan điểm xã hội xây dựng được tiến hành vào năm 2015, nghiên cứu này khám phá cách ra quyết định tuyển chọn nội trú diễn ra như thế nào trong bốn chuyên khoa tại hai khu vực ở Hà Lan. Dữ liệu bao gồm biên bản các cuộc họp ra quyết định và các cuộc phỏng vấn trực tiếp với các thành viên ủy ban trước và sau các cuộc họp ra quyết định của nhóm. Các ứng viên đã phải vật lộn để thể hiện bản thân một cách thuận lợi vì họ phải cân bằng giữa việc tuân thủ các quy tắc và trở nên chân thực; giữa việc hòa nhập và nổi bật. Mặc dù các ủy ban tuyển sinh có lập trường chào đón sự đa dạng, nhưng các hoạt động của họ vô tình ngăn cản họ tuyển dụng các ứng viên thiểu số không được đại diện (URM). Mặc dù việc đàm phán tuyển sinh là khó khăn đối với tất cả các ứng viên, nhưng đối với các ứng viên URM thì việc này được cho là phức tạp hơn. Điều này dường như đang có ảnh hưởng tiêu cực đến việc đạt được sự đa dạng của lực lượng lao động. Những niềm tin hiện tại khiến các ủy ban lầm tưởng rằng họ đang hành động công bằng, nhưng thực tế có thể biện minh cho các hoạt động thiên vị. Nhận thức về vai trò của các thành viên ủy ban trong các quá trình này là bước đầu tiên thiết yếu."} +{"idx": 1870608, "en": "OBJECTIVE To describe the clinical and serological characteristics of patients with SLE who reached a state of sustained remission for more than 10 years in the absence of treatment. METHODS From a retrospective cohort of 2121 patients, 44 cases with sustained remission (PtRem) were identified and compared with 88 patients whose course has been chronically active (PtAct).The clinical and serological characteristics were analyzed, as well as the treatment of each group at the beginning of the disease and during its evolution. RESULTS Older age at disease onset was associated with a tendency to reach a state of prolonged remission. These patients also had a higher frequency of thrombocytopenia at the beginning of the disease 34.1% vs 10.2% (p < 0.001). PtAct had a significantly higher initial SLEDAI compared with cases (10.4 ± 5.6 vs 14.1 ± 5.8; p < 0.001). PtRem had a higher initial frequency of anti-β2 GP1 IgG antibodies. Also, 25% of these patients were serologically active. We did not find differences in the initial treatment between both groups. The accumulated damage measured by SLICC/ACR damage index at the end of the study was significantly less in the patients who remained in prolonged remission. CONCLUSIONS Although patients with SLE who achieve prolonged remission have some different characteristics at baseline compared with PtAct, it is not possible to identify a characteristic phenotype for the former. Achieving a state of prolonged remission should always be the goal in patients with SLE. Key Points • SLE patients can reach a very prolonged state of remission, free of treatment, including antimalarials, for at least 10 years. • Venous thromboembolism and thrombocytopenia are commonly present in patients that achieved remission. • The presence of serological markers of activity, even after 10 years in remission, is a risk factor for relapse.", "gemini": "MỤC TIÊU Mô tả các đặc điểm lâm sàng và huyết thanh của những bệnh nhân mắc SLE đạt trạng thái thuyên giảm kéo dài hơn 10 năm khi không điều trị. PHƯƠNG PHÁP Từ một nhóm bệnh nhân hồi cứu gồm 2121 bệnh nhân, 44 trường hợp thuyên giảm kéo dài (PtRem) đã được xác định và so sánh với 88 bệnh nhân có diễn biến hoạt động mãn tính (PtAct). Các đặc điểm lâm sàng và huyết thanh đã được phân tích, cũng như phương pháp điều trị của từng nhóm khi bắt đầu mắc bệnh và trong quá trình tiến triển của bệnh. KẾT QUẢ Tuổi khởi phát bệnh cao hơn có liên quan đến xu hướng đạt trạng thái thuyên giảm kéo dài. Những bệnh nhân này cũng có tần suất giảm tiểu cầu cao hơn khi khởi phát bệnh 34,1% so với 10,2% (p < 0,001). PtAct có SLEDAI ban đầu cao hơn đáng kể so với các trường hợp (10,4 ± 5,6 so với 14,1 ± 5,8; p < 0,001). PtRem có tần suất ban đầu cao hơn của kháng thể anti-β2 GP1 IgG. Ngoài ra, 25% những bệnh nhân này hoạt động về mặt huyết thanh. Chúng tôi không tìm thấy sự khác biệt trong việc điều trị ban đầu giữa hai nhóm. Tổn thương tích lũy được đo bằng chỉ số tổn thương SLICC/ACR vào cuối nghiên cứu thấp hơn đáng kể ở những bệnh nhân vẫn trong tình trạng thuyên giảm kéo dài. KẾT LUẬN Mặc dù những bệnh nhân mắc SLE đạt được trạng thái thuyên giảm kéo dài có một số đặc điểm khác nhau khi mới bắt đầu so với PtAct, nhưng không thể xác định được kiểu hình đặc trưng cho nhóm trước. Đạt được trạng thái thuyên giảm kéo dài luôn phải là mục tiêu ở những bệnh nhân mắc SLE. Những điểm chính • Bệnh nhân SLE có thể đạt được trạng thái thuyên giảm rất kéo dài, không cần điều trị, bao gồm thuốc chống sốt rét, trong ít nhất 10 năm. • Thuyên tắc huyết khối tĩnh mạch và giảm tiểu cầu thường gặp ở những bệnh nhân đạt được trạng thái thuyên giảm. • Sự hiện diện của các dấu hiệu hoạt động huyết thanh, ngay cả sau 10 năm thuyên giảm, là một yếu tố nguy cơ tái phát."} +{"idx": 1870609, "en": "The size (volume or mass) of the olfactory bulbs in relation to the whole brain has been used as a neuroanatomical proxy for olfactory capability in a range of vertebrates, including fishes. Here, we use diffusible iodine-based contrast-enhanced computed tomography (diceCT) to test the value of this novel bioimaging technique for generating accurate measurements of the relative volume of the main olfactory brain areas (olfactory bulbs, peduncles, and telencephalon) and to describe the morphological organisation of the ascending olfactory pathway in model fish species from two taxa, the brownbanded bamboo shark Chiloscyllium punctatum and the common goldfish Carassius auratus. We also describe the arrangement of primary projections to the olfactory bulb and secondary projections to the telencephalon in both species. Our results identified substantially larger olfactory bulbs and telencephalon in C. punctatum compared to C. auratus (comprising approximately 5.2% vs. 1.8%, and 51.8% vs. 11.8% of the total brain volume, respectively), reflecting differences between taxa, but also possibly in the role of olfaction in the sensory ecology of these species. We identified segregated primary projections to the bulbs, associated with a compartmentalised olfactory bulb in C. punctatum, which supports previous findings in elasmobranch fishes. DiceCT imaging has been crucial for visualising differences in the morphological organisation of the olfactory system of both model species. We consider comparative neuroanatomical studies between representative species of both elasmobranch and teleost fish groups are fundamental to further our understanding of the evolution of the olfactory system in early vertebrates and the neural basis of olfactory abilities.", "gemini": "Kích thước (thể tích hoặc khối lượng) của các củ khứu giác so với toàn bộ não đã được sử dụng như một đại diện thần kinh giải phẫu cho khả năng khứu giác ở nhiều loài động vật có xương sống, bao gồm cả cá. Ở đây, chúng tôi sử dụng chụp cắt lớp vi tính tăng cường độ tương phản dựa trên iốt khuếch tán (diceCT) để kiểm tra giá trị của kỹ thuật chụp ảnh sinh học mới này trong việc tạo ra các phép đo chính xác về thể tích tương đối của các vùng não khứu giác chính (củ khứu giác, cuống và đại não) và để mô tả tổ chức hình thái của đường dẫn khứu giác hướng lên ở các loài cá mẫu từ hai phân loại, cá mập tre nâu Chiloscyllium punctatum và cá vàng thông thường Carassius auratus. Chúng tôi cũng mô tả sự sắp xếp của các đường chiếu chính đến củ khứu giác và các đường chiếu thứ cấp đến đại não ở cả hai loài. Kết quả của chúng tôi xác định được các củ khứu giác và đại não lớn hơn đáng kể ở C. punctatum so với C. auratus (chiếm khoảng 5,2% so với 1,8% và 51,8% so với 11,8% tổng thể tích não, tương ứng), phản ánh sự khác biệt giữa các phân loại, nhưng cũng có thể là vai trò của khứu giác trong sinh thái cảm giác của các loài này. Chúng tôi đã xác định được các đường chiếu chính phân tách đến các củ, liên quan đến một củ khứu giác được chia thành nhiều ngăn ở C. punctatum, điều này hỗ trợ các phát hiện trước đây ở cá sụn. Chụp ảnh DiceCT rất quan trọng để hình dung sự khác biệt trong tổ chức hình thái của hệ thống khứu giác của cả hai loài mẫu. Chúng tôi coi các nghiên cứu thần kinh giải phẫu so sánh giữa các loài đại diện của cả nhóm cá sụn và cá xương là cơ bản để hiểu sâu hơn về sự tiến hóa của hệ thống khứu giác ở động vật có xương sống thời kỳ đầu và cơ sở thần kinh của khả năng khứu giác."} +{"idx": 1870610, "en": "BACKGROUND Even though the need has been challenged, admitting patients to an intensive care or medium care unit (ICU/MCU) after adult supratentorial tumor craniotomy remains common practice. We have introduced a \"no ICU, unless\" policy for tumor craniotomy patients and evaluate costs, complications, and length of stay. METHODS A prospective cohort study was performed comparing patients that underwent tumor craniotomy for supratentorial tumors during 2 years after introduction of the new policy with the year before. RESULTS A reduction in ICU/MCU admittance from 88 to 23% of patients was found resulting in 13% cost reduction. Also, the new policy resulted in a 1.4-day shorter post-operative length of stay. Minor complications were reduced, while major complications remained the same. All major complications are reviewed. CONCLUSIONS We show that routine post-operative ICU/MCU admittance after tumor craniotomy does not reduce complications, but actually interferes with recovery of our patients. Changing the paradigm results in earlier discharge and cost reduction.", "gemini": "BỐI CẢNH Mặc dù nhu cầu đã bị thách thức, nhưng việc đưa bệnh nhân vào đơn vị chăm sóc đặc biệt hoặc đơn vị chăm sóc trung bình (ICU/MCU) sau khi cắt sọ não u ở người lớn vẫn là thông lệ phổ biến. Chúng tôi đã đưa ra chính sách \"không vào ICU, trừ khi\" đối với những bệnh nhân cắt sọ não u và đánh giá chi phí, biến chứng và thời gian nằm viện. PHƯƠNG PHÁP Một nghiên cứu theo dõi tiến cứu đã được thực hiện để so sánh những bệnh nhân đã cắt sọ não u ở các khối u trên lều trong 2 năm sau khi đưa ra chính sách mới với năm trước đó. KẾT QUẢ Phát hiện thấy tỷ lệ nhập viện ICU/MCU giảm từ 88 xuống 23% bệnh nhân, dẫn đến giảm 13% chi phí. Ngoài ra, chính sách mới dẫn đến thời gian nằm viện sau phẫu thuật ngắn hơn 1,4 ngày. Các biến chứng nhỏ đã giảm, trong khi các biến chứng lớn vẫn như cũ. Tất cả các biến chứng lớn đều được xem xét. KẾT LUẬN Chúng tôi chỉ ra rằng việc nhập viện ICU/MCU sau phẫu thuật cắt sọ não u thông thường không làm giảm các biến chứng, mà thực sự ảnh hưởng đến quá trình phục hồi của bệnh nhân của chúng tôi. Thay đổi mô hình dẫn đến xuất viện sớm hơn và giảm chi phí."} +{"idx": 1870611, "en": "BACKGROUND The discovery of an unruptured intracranial aneurysm creates a dilemma between observation and treatment. Neurosurgeons' routines for risk assessment and treatment decision-making are unknown. The position of evidence-based medicine in European neurosurgery is considered to be weak, high-grade guidelines do not exist and variations between institutions are probable. We aimed to explore European neurosurgeons' management routines for newly discovered unruptured intracranial aneurysms. METHODS In cooperation with the European Association of Neurosurgical Societies (EANS), we conducted an online, cross-sectional survey of 420 European neurosurgeons during Spring/Summer 2016 (1533 non-Norwegians invited through the EANS, and 16 Norwegians invited through heads of departments because of the need for additional information for a separate study). We asked about demographic variables, routines for management and risk assessment of newly discovered unruptured intracranial aneurysms and presented a case. We collected information about gross domestic product (GDP) per capita from the International Monetary Fund. RESULTS The response rate to the invite from the EANS was 26%, with respondents from 47 countries. More than half of the respondents (n = 226 [54%]) reported that their department treated less than 25 unruptured aneurysms yearly. Forty percent said their department used aneurysm size cut-off to guide treatment decisions, with a mean size of 6 mm. Presented with a case, respondents from countries with a lower GDP per capita recommended intervention more often than respondents from higher-income countries. Vascular neurosurgeons more commonly recommended observation. CONCLUSION The answers to this self-reported survey indicate that many centers have a treatment volume lower than recommended by international guidelines, and that there are socioeconomic differences in care. Better documentation of treatment and outcome, for example with clinical quality registries, is needed to drive improvements of care.", "gemini": "BỐI CẢNH Việc phát hiện ra một phình động mạch nội sọ chưa vỡ tạo ra một tình huống tiến thoái lưỡng nan giữa quan sát và điều trị. Các thói quen của bác sĩ phẫu thuật thần kinh để đánh giá rủi ro và đưa ra quyết định điều trị vẫn chưa được biết. Vị trí của y học dựa trên bằng chứng trong phẫu thuật thần kinh châu Âu được coi là yếu, không có hướng dẫn cấp cao và có thể có sự khác biệt giữa các cơ sở. Chúng tôi nhằm mục đích khám phá các thói quen quản lý của các bác sĩ phẫu thuật thần kinh châu Âu đối với các phình động mạch nội sọ chưa vỡ mới được phát hiện. PHƯƠNG PHÁP Hợp tác với Hiệp hội các hội phẫu thuật thần kinh châu Âu (EANS), chúng tôi đã tiến hành một cuộc khảo sát trực tuyến, cắt ngang đối với 420 bác sĩ phẫu thuật thần kinh châu Âu vào mùa xuân/mùa hè năm 2016 (1533 người không phải người Na Uy được mời thông qua EANS và 16 người Na Uy được mời thông qua các trưởng khoa vì cần thêm thông tin cho một nghiên cứu riêng biệt). Chúng tôi đã hỏi về các biến số nhân khẩu học, các thói quen quản lý và đánh giá rủi ro của các phình động mạch nội sọ chưa vỡ mới được phát hiện và trình bày một trường hợp. Chúng tôi thu thập thông tin về tổng sản phẩm quốc nội (GDP) bình quân đầu người từ Quỹ Tiền tệ Quốc tế. KẾT QUẢ Tỷ lệ phản hồi lời mời từ EANS là 26%, với những người trả lời đến từ 47 quốc gia. Hơn một nửa số người trả lời (n = 226 [54%]) báo cáo rằng khoa của họ điều trị ít hơn 25 phình động mạch chưa vỡ hàng năm. Bốn mươi phần trăm cho biết khoa của họ sử dụng ngưỡng kích thước phình động mạch để hướng dẫn các quyết định điều trị, với kích thước trung bình là 6 mm. Khi trình bày một trường hợp, những người trả lời từ các quốc gia có GDP bình quân đầu người thấp hơn thường khuyến nghị can thiệp nhiều hơn so với những người trả lời từ các quốc gia có thu nhập cao hơn. Các bác sĩ phẫu thuật thần kinh mạch máu thường khuyến nghị theo dõi hơn. KẾT LUẬN Các câu trả lời cho cuộc khảo sát tự báo cáo này chỉ ra rằng nhiều trung tâm có khối lượng điều trị thấp hơn khuyến nghị của các hướng dẫn quốc tế và có sự khác biệt về kinh tế xã hội trong chăm sóc. Cần có tài liệu tốt hơn về điều trị và kết quả, ví dụ như với sổ đăng ký chất lượng lâm sàng, để thúc đẩy cải thiện việc chăm sóc."} +{"idx": 1870612, "en": "Genome-wide identification, tissue-specific expression analysis and functional characterization of selected genes containing the pear Pollen Olea europaea I domain reveal their roles in pollen tube growth. Genes containing the Pollen Olea europaea I (POE) domain play crucial roles in diverse growth and developmental processes. Nevertheless, the specific functions of POE family members in progression of pollen tube growth (PTG) remain uncharacterized. We identified 45 PbrPOE genes in the pear (Pyrus bretschneideri) genome, clustered into seven subclasses. PbrPOE genes contained 1 to 11 exons and 0 to 10 introns, with exon/intron structure mostly conserved within each subclass. Whole-genome duplication has mainly contributed to the duplication pattern of PbrPOE genes in pear. Expression profiles of 45 PbrPOE genes in 12 different pear tissues revealed that six PbrPOE genes (PbrPOE6, 12, 21, 29, 35 and 41) of subclass B were highly expressed during the growth of the pear pollen tube in vitro. PbrPOE21 was selected for further functional analysis on the basis of its high and differential expression pattern in pollen. Antisense oligodeoxynucleotide assays demonstrated that PTG was augmented in vitro when PbrPOE21 expression was significantly inhibited. Moreover, pollen tube length in vitro was reduced when PbrPOE21 was transitorily over-expressed using particle bombardment technology. Exogenous PbrPOE21 recombinant protein inhibited PTG in vitro at an optimum concentration of 1.8 µM. PbrPOE21 also affected reactive oxygen species content in the pear pollen tube apex. We suggest that PbrPOE21 inhibits PTG in vitro by altering apical reactive oxygen species content.", "gemini": "Việc xác định trên toàn bộ hệ gen, phân tích biểu hiện đặc hiệu theo mô và đặc điểm chức năng của các gen được chọn có chứa miền lê Pollen Olea europaea I cho thấy vai trò của chúng trong sự phát triển của ống phấn. Các gen chứa miền Pollen Olea europaea I (POE) đóng vai trò quan trọng trong nhiều quá trình tăng trưởng và phát triển. Tuy nhiên, các chức năng cụ thể của các thành viên họ POE trong quá trình phát triển của ống phấn (PTG) vẫn chưa được xác định. Chúng tôi đã xác định được 45 gen PbrPOE trong bộ gen của quả lê (Pyrus bretschneideri), được phân nhóm thành bảy phân lớp. Các gen PbrPOE chứa từ 1 đến 11 exon và từ 0 đến 10 intron, với cấu trúc exon/intron chủ yếu được bảo tồn trong mỗi phân lớp. Sự nhân đôi toàn bộ hệ gen chủ yếu góp phần vào kiểu nhân đôi của các gen PbrPOE ở quả lê. Hồ sơ biểu hiện của 45 gen PbrPOE trong 12 mô lê khác nhau cho thấy sáu gen PbrPOE (PbrPOE6, 12, 21, 29, 35 và 41) của phân lớp B được biểu hiện ở mức cao trong quá trình phát triển của ống phấn lê trong ống nghiệm. PbrPOE21 được chọn để phân tích chức năng sâu hơn trên cơ sở kiểu biểu hiện cao và khác biệt của nó trong phấn hoa. Các xét nghiệm oligodeoxynucleotide antisense chứng minh rằng PTG tăng lên trong ống nghiệm khi biểu hiện PbrPOE21 bị ức chế đáng kể. Hơn nữa, chiều dài ống phấn trong ống nghiệm giảm khi PbrPOE21 được biểu hiện quá mức tạm thời bằng công nghệ bắn phá hạt. Protein tái tổ hợp PbrPOE21 ngoại sinh ức chế PTG trong ống nghiệm ở nồng độ tối ưu là 1,8 µM. PbrPOE21 cũng ảnh hưởng đến hàm lượng các loài oxy phản ứng trong đỉnh ống phấn lê. Chúng tôi cho rằng PbrPOE21 ức chế PTG trong ống nghiệm bằng cách làm thay đổi hàm lượng các loài oxy phản ứng ở đỉnh."} +{"idx": 1870613, "en": "PURPOSE Obstructive sleep apnea (OSA) is a complex disease in which phenotypic analysis and understanding pathological mechanisms facilitate personalized treatment and outcomes. However, the pathophysiology responsible for this robust observation is incompletely understood. The objective of the present work was to review how respiratory center regulation varies during sleep and wakeness in patients with OSA. DATA SOURCES We searched for relevant articles up to December 31, 2019 in PubMed database. METHODS This review examines the current literature on the characteristics of respiratory center regulation during wakefulness and sleep in OSA, detection method, and phenotypic treatment for respiratory center regulation. RESULTS Mechanisms for ventilatory control system instability leading to OSA include different sleep stages in chemoresponsiveness to hypoxia and hypercapnia and different chemosensitivity at different time. One can potentially stabilize the breathing center in sleep-related breathing disorders by identifying one or more of these pathophysiological mechanisms. CONCLUSIONS Advancing mechanism research in OSA will guide symptom research and provide alternate and novel opportunities for effective treatment for patients with OSA.", "gemini": "MỤC ĐÍCH Ngưng thở khi ngủ do tắc nghẽn (OSA) là một bệnh phức tạp trong đó việc phân tích kiểu hình và hiểu các cơ chế bệnh lý tạo điều kiện cho việc điều trị và kết quả được cá nhân hóa. Tuy nhiên, cơ chế bệnh sinh chịu trách nhiệm cho quan sát mạnh mẽ này vẫn chưa được hiểu đầy đủ. Mục tiêu của công trình hiện tại là xem xét cách điều hòa trung tâm hô hấp thay đổi trong khi ngủ và thức ở những bệnh nhân mắc OSA. NGUỒN DỮ LIỆU Chúng tôi đã tìm kiếm các bài báo có liên quan cho đến ngày 31 tháng 12 năm 2019 trong cơ sở dữ liệu PubMed. PHƯƠNG PHÁP Đánh giá này xem xét các tài liệu hiện tại về các đặc điểm của điều hòa trung tâm hô hấp trong khi thức và ngủ ở OSA, phương pháp phát hiện và điều trị kiểu hình cho điều hòa trung tâm hô hấp. KẾT QUẢ Các cơ chế gây mất ổn định hệ thống kiểm soát thông khí dẫn đến OSA bao gồm các giai đoạn ngủ khác nhau về khả năng đáp ứng hóa học với tình trạng thiếu oxy và tăng CO2 và độ nhạy hóa học khác nhau tại các thời điểm khác nhau. Người ta có thể ổn định trung tâm hô hấp trong các rối loạn hô hấp liên quan đến giấc ngủ bằng cách xác định một hoặc nhiều cơ chế bệnh sinh này. KẾT LUẬN Việc thúc đẩy nghiên cứu cơ chế ở OSA sẽ hướng dẫn nghiên cứu triệu chứng và cung cấp các cơ hội thay thế và mới để điều trị hiệu quả cho bệnh nhân OSA."} +{"idx": 1870614, "en": "Pain and depression are often co-existing pathological states that promote mutual severity resulting in limited efficacy of current treatment strategies. Thus, there is a need to develop an efficacious alternate treatment regimen for pain-depression dyad. Skimmetin and osthole are molecules of natural origin that have been explored for an anti-hyperglycemic, anti-bacterial, anti-fungal, and anti-diabetic activities in preclinical studies. in animal models. The current study has been designed to explore the beneficial effect of skimmetin/osthole in reserpine-induced pain-depression dyad in mice. Female Swiss albino mice (n = 6) were challenged with reserpine (0.5 mg/kg s.c.) for the first 3 days to induce a pain-depression dyad-like state. Skimmetin (10 mg/kg i.p.) and osthole (10 mg/kg i.p.) were administered for 5 days consecutively, starting from the first day of study. Reserpine treatment significantly reduced the pain threshold in the pressure application measurement (PAM) and electronic von frey (eVF) test. In forced swim test (FST) and Morris water maze (MWM) test mice displayed an increased immobility time and latency to reach platform respectively. Biochemical results showed an increased level of TNF-α, IL-1β, TBARS, glutamate, and reduced level of GSH, norepinephrine, and serotonin in the reserpine treated group. Reserpine treatment also increased brain MAO-A activity. Skimmetin/osthole treatment was found to attenuate the behavioral and biochemical alterations induced by reserpine. The results of the current investigation delineated that skimmetin/osthole may exert anti-nociceptive, anti-depressant, and improved cognition via inhibiting inflammatory and oxidative stress-mediated neurotransmitter dysregulation.", "gemini": "Đau và trầm cảm thường là các trạng thái bệnh lý cùng tồn tại, thúc đẩy mức độ nghiêm trọng lẫn nhau dẫn đến hiệu quả hạn chế của các chiến lược điều trị hiện tại. Do đó, cần phải phát triển một chế độ điều trị thay thế hiệu quả cho cặp đau-trầm cảm. Skimmetin và osthole là các phân tử có nguồn gốc tự nhiên đã được khám phá về hoạt tính chống tăng đường huyết, kháng khuẩn, kháng nấm và chống tiểu đường trong các nghiên cứu tiền lâm sàng. ở các mô hình động vật. Nghiên cứu hiện tại được thiết kế để khám phá tác dụng có lợi của skimmetin/osthole trong cặp đau-trầm cảm do reserpine gây ra ở chuột. Chuột bạch tạng Swiss cái (n = 6) được thử thách với reserpine (0,5 mg/kg dưới da) trong 3 ngày đầu tiên để gây ra trạng thái giống như cặp đau-trầm cảm. Skimmetin (10 mg/kg tiêm phúc mạc) và osthole (10 mg/kg tiêm phúc mạc) được dùng trong 5 ngày liên tiếp, bắt đầu từ ngày đầu tiên của nghiên cứu. Điều trị bằng reserpine làm giảm đáng kể ngưỡng đau trong phép đo áp lực (PAM) và thử nghiệm von frey điện tử (eVF). Trong thử nghiệm bơi cưỡng bức (FST) và thử nghiệm mê cung Morris (MWM), chuột lần lượt biểu hiện thời gian bất động và độ trễ tăng để đạt đến bục. Kết quả sinh hóa cho thấy mức TNF-α, IL-1β, TBARS, glutamate tăng và mức GSH, norepinephrine và serotonin giảm trong nhóm được điều trị bằng reserpine. Điều trị bằng reserpine cũng làm tăng hoạt tính MAO-A của não. Người ta thấy rằng điều trị bằng skimmetin/osthole làm giảm các thay đổi về hành vi và sinh hóa do reserpine gây ra. Kết quả của cuộc điều tra hiện tại mô tả rằng skimmetin/osthole có thể phát huy tác dụng giảm đau, chống trầm cảm và cải thiện nhận thức thông qua việc ức chế sự rối loạn dẫn truyền thần kinh do viêm và stress oxy hóa."} +{"idx": 1870615, "en": "The present study aimed to analyze the impact of tartrazine (T) and crocin (Cr) applications on the pancreas tissues of the Wistar rats. A total of 40 Wistar rats were randomly divided into 4 groups with 10 rats in each group, including the Control, T, Cr, and T + Cr groups. After 3 weeks of application, the pancreatic tissues of the rats were removed under anesthesia and rat blood samples were obtained. Tissues were analyzed with biochemical and histopathological methods. It was determined that T administration increased malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), glucose, triglyceride, LDL, VLDL, and total cholesterol levels. However, it decreased reduced glutathione (GSH), total antioxidant status (TAS), superoxide dismutase (SOD), catalase (CAT), and HDL levels when compared with the other groups. It was observed that Cr administration significantly increased GSH, SOD, CAT, TAS, and HDL levels when compared with the control group. In the T group, histopathological changes were observed in pancreatic tissue, leading to damages in exocrine pancreas and islets of Langerhans and increased caspase-3 immunoreactivity (p ≤ 0.001). Co-administration of Cr and T brought the biochemical and histopathological findings closer to the control group levels. The administration of T induced damage in the pancreas with the administered dose and frequency. Cr can increase the antioxidant capacity in pancreas tissue. Co-administration of T and Cr contributed to the reduction of the toxic effects induced by T. It could be suggested that Cr administration ameliorated T toxicity.", "gemini": "Nghiên cứu hiện tại nhằm phân tích tác động của việc sử dụng tartrazine (T) và crocin (Cr) lên mô tụy của chuột Wistar. Tổng cộng 40 con chuột Wistar được chia ngẫu nhiên thành 4 nhóm, mỗi nhóm 10 con, bao gồm nhóm Đối chứng, nhóm T, nhóm Cr và nhóm T + Cr. Sau 3 tuần sử dụng, mô tụy của chuột được lấy ra dưới gây mê và lấy mẫu máu chuột. Mô được phân tích bằng phương pháp sinh hóa và mô bệnh học. Người ta xác định rằng việc sử dụng T làm tăng malondialdehyde (MDA), trạng thái oxy hóa toàn phần (TOS), chỉ số stress oxy hóa (OSI), glucose, triglyceride, LDL, VLDL và mức cholesterol toàn phần. Tuy nhiên, nó làm giảm glutathione khử (GSH), trạng thái chống oxy hóa toàn phần (TAS), superoxide dismutase (SOD), catalase (CAT) và mức HDL khi so sánh với các nhóm khác. Người ta quan sát thấy rằng việc sử dụng Cr làm tăng đáng kể mức GSH, SOD, CAT, TAS và HDL khi so sánh với nhóm đối chứng. Ở nhóm T, những thay đổi về mô bệnh học được quan sát thấy ở mô tụy, dẫn đến tổn thương ở tụy ngoại tiết và tiểu đảo Langerhans và tăng phản ứng miễn dịch caspase-3 (p ≤ 0,001). Việc dùng đồng thời Cr và T đưa các phát hiện về sinh hóa và mô bệnh học gần hơn với mức của nhóm đối chứng. Việc sử dụng T gây tổn thương ở tụy với liều và tần suất đã sử dụng. Cr có thể làm tăng khả năng chống oxy hóa ở mô tụy. Việc sử dụng đồng thời T và Cr góp phần làm giảm các tác động độc hại do T gây ra. Có thể gợi ý rằng việc sử dụng Cr làm giảm độc tính của T."} +{"idx": 1870616, "en": "Air pollution is an important cause of non-communicable diseases globally with particulate matter (PM) as one of the main air pollutants. PM is composed of microscopic particles that contain a mixture of chemicals and biological elements that can be harmful to human health. The aerodynamic diameter of PM facilitates their deposition when inhaled. For instance, coarse PM having a diameter of < 10 μm is deposited mainly in the large conducting airways, but PM of < 2.5 μm can cross the alveolar-capillary barrier, traveling to other organs within the body. Epidemiological studies have shown the association between PM exposure and risk of disease, namely those of the respiratory system such as lung cancer, asthma, and chronic obstructive pulmonary disease (COPD). However, cardiovascular and neurological diseases have also been reported, including hypertension, atherosclerosis, acute myocardial infarction, stroke, loss of cognitive function, anxiety, and Parkinson's and Alzheimer's diseases. Inflammation is a common hallmark in the pathogenesis of many of these diseases associated with exposure to a variety of air pollutants, including PM. This review focuses on the main effects of PM on human health, with an emphasis on the role of inflammation.", "gemini": "Ô nhiễm không khí là một nguyên nhân quan trọng gây ra các bệnh không lây nhiễm trên toàn cầu với hạt vật chất (PM) là một trong những chất gây ô nhiễm không khí chính. PM bao gồm các hạt vi mô chứa hỗn hợp các hóa chất và các thành phần sinh học có thể gây hại cho sức khỏe con người. Đường kính khí động học của PM tạo điều kiện thuận lợi cho chúng lắng đọng khi hít vào. Ví dụ, PM thô có đường kính <10 μm chủ yếu lắng đọng ở các đường dẫn khí lớn, nhưng PM <2,5 μm có thể đi qua hàng rào mao mạch phế nang, di chuyển đến các cơ quan khác trong cơ thể. Các nghiên cứu dịch tễ học đã chỉ ra mối liên quan giữa phơi nhiễm PM và nguy cơ mắc bệnh, cụ thể là các bệnh về hệ hô hấp như ung thư phổi, hen suyễn và bệnh phổi tắc nghẽn mạn tính (COPD). Tuy nhiên, các bệnh tim mạch và thần kinh cũng đã được báo cáo, bao gồm tăng huyết áp, xơ vữa động mạch, nhồi máu cơ tim cấp, đột quỵ, mất chức năng nhận thức, lo lắng và bệnh Parkinson và Alzheimer. Viêm là một dấu hiệu phổ biến trong quá trình sinh bệnh của nhiều bệnh liên quan đến việc tiếp xúc với nhiều chất gây ô nhiễm không khí, bao gồm cả PM. Tổng quan này tập trung vào các tác động chính của PM đối với sức khỏe con người, nhấn mạnh vai trò của tình trạng viêm."} +{"idx": 1870617, "en": "This paper investigates the existence of the Environmental Kuznets Curve for carbon dioxide emissions in Ghana. The causal relationship between economic growth and carbon dioxide emissions being examined and the short-run and long-run parameters of the estimated vector autoregressive models are found to be unstable. This necessitated the use of a time-varying approach and the rolling window Granger causality test to investigate the causal relationship between economic growth and carbon dioxide emissions. We find that GDP has a positive effect on carbon dioxide emissions in the sample periods where GDP Granger causes carbon dioxide emissions. Significant feedbacks from the environment to the economy are observed, with carbon dioxide emissions having a positive effect on GDP in most of the subsample periods. The empirical results show that the Environmental Kuznets Curve for carbon dioxide emissions for Ghana is upward sloping, contrary to the standard Environmental Kuznets Curve theory which postulates an inverted \"U\"-shaped relationship between economic growth and environmental degradation. The implication of the result is that carbon dioxide emissions increase with increases in GDP. The study recommends that existing policies designed to ensure environmental sustainability and hence control carbon dioxide emissions need to be strengthened and enforced to ensure that the rapid urbanization and industrialization of the Ghanaian economy does not come at a cost to the quality of the environment.", "gemini": "Bài báo này nghiên cứu sự tồn tại của Đường cong Kuznets Môi trường đối với lượng khí thải carbon dioxide ở Ghana. Mối quan hệ nhân quả giữa tăng trưởng kinh tế và lượng khí thải carbon dioxide đang được xem xét và các tham số ngắn hạn và dài hạn của các mô hình hồi quy tự hồi quy ước tính được phát hiện là không ổn định. Điều này đòi hỏi phải sử dụng phương pháp thay đổi theo thời gian và kiểm tra nhân quả Granger trên cửa sổ trượt để nghiên cứu mối quan hệ nhân quả giữa tăng trưởng kinh tế và lượng khí thải carbon dioxide. Chúng tôi thấy rằng GDP có tác động tích cực đến lượng khí thải carbon dioxide trong các giai đoạn mẫu mà GDP Granger gây ra lượng khí thải carbon dioxide. Phản hồi đáng kể từ môi trường đến nền kinh tế được quan sát thấy, với lượng khí thải carbon dioxide có tác động tích cực đến GDP trong hầu hết các giai đoạn mẫu phụ. Các kết quả thực nghiệm cho thấy Đường cong Kuznets Môi trường đối với lượng khí thải carbon dioxide ở Ghana có xu hướng đi lên, trái ngược với lý thuyết Đường cong Kuznets Môi trường tiêu chuẩn, lý thuyết này đưa ra mối quan hệ hình chữ \"U\" đảo ngược giữa tăng trưởng kinh tế và suy thoái môi trường. Ý nghĩa của kết quả là lượng khí thải carbon dioxide tăng theo sự gia tăng của GDP. Nghiên cứu khuyến nghị rằng các chính sách hiện hành được thiết kế để đảm bảo tính bền vững của môi trường và do đó kiểm soát lượng khí thải carbon dioxide cần được tăng cường và thực thi để đảm bảo rằng quá trình đô thị hóa và công nghiệp hóa nhanh chóng của nền kinh tế Ghana không phải trả giá bằng chất lượng của môi trường."} +{"idx": 1870618, "en": "To overcome the weaknesses of traditional landfills, a modified aerobic landfill concept with intermediate covers of coarse material between waste layers functioning as facilities of drainage and aeration has been proposed recently. In this study, a one-dimensional coupled model, including aerobic biodegradation, oxygen diffusion, and advection, is proposed to describe oxygen distribution in this new type of landfill. Homotopy analysis method and perturbation method are applied to solve this model at passive aeration and active aeration, respectively. The model has six input variables, that is, oxygen diffusion coefficient, gas permeability, maximum oxygen consumption rate, layer thickness of waste, and injection pressure and extraction pressure. A combination of their typical values gives rise to over 700,000 scenarios which can be calculated by the proposed solution. The coupled effect of the above variables on oxygen migration is quantitatively investigated, followed by an estimation formula of the minimum oxygen concentration in waste layer. The maximum waste layer thickness is defined as a function of other variables for a given aeration target of oxygen volume concentration larger than 5%. A generalized design method of waste layer thickness, injection pressure, and extraction pressure is then developed for the newly proposed modified layered aerobic landfill, which can promote its popularization and application.", "gemini": "Để khắc phục những điểm yếu của bãi chôn lấp truyền thống, gần đây đã có đề xuất về một khái niệm bãi chôn lấp hiếu khí được cải tiến với các lớp phủ trung gian bằng vật liệu thô giữa các lớp chất thải hoạt động như các cơ sở thoát nước và sục khí. Trong nghiên cứu này, một mô hình ghép một chiều, bao gồm phân hủy sinh học hiếu khí, khuếch tán oxy và đối lưu, được đề xuất để mô tả phân phối oxy trong loại bãi chôn lấp mới này. Phương pháp phân tích đồng dạng và phương pháp nhiễu được áp dụng để giải mô hình này ở trạng thái sục khí thụ động và sục khí chủ động. Mô hình có sáu biến đầu vào, đó là hệ số khuếch tán oxy, độ thấm khí, tốc độ tiêu thụ oxy tối đa, độ dày lớp chất thải, áp suất phun và áp suất chiết. Sự kết hợp các giá trị điển hình của chúng tạo ra hơn 700.000 kịch bản có thể được tính toán bằng giải pháp được đề xuất. Hiệu ứng kết hợp của các biến trên đối với quá trình di chuyển oxy được nghiên cứu định lượng, tiếp theo là công thức ước tính nồng độ oxy tối thiểu trong lớp chất thải. Độ dày lớp chất thải tối đa được xác định là một hàm của các biến khác đối với mục tiêu sục khí nhất định có nồng độ thể tích oxy lớn hơn 5%. Sau đó, một phương pháp thiết kế tổng quát về độ dày lớp chất thải, áp suất phun và áp suất chiết được phát triển cho bãi chôn lấp hiếu khí có lớp phủ được đề xuất mới, có thể thúc đẩy việc phổ biến và ứng dụng của bãi chôn lấp này."} +{"idx": 1870619, "en": "The eriophyoid mite Aceria massalongoi (Canestrini) was collected from globoid leaf galls on severely injured chaste trees, Vitex agnus-castus L. (Lamiaceae), in Bari and Bernalda (southern Italy), and on the Ionian island Leukade (Greece). Female, male and nymph were described in detail, following the current morphometric descriptive scheme, supplementing older and incomplete descriptions. Molecular characterization of A. massalongoi from Italy and Greece was conducted by amplifying and sequencing the ribosomal ITS, the D2-D3 expansion domains of the 28S rRNA gene and the mitochondrial COI, for the first time. Phylogenetic trees based on the three molecular markers showed congruent results, confirming that Italian and Greek A. massalongoi populations are the same species that cluster together with some intraspecific variability. Galls, ranging from 0.5 to 2.8 mm in diameter, were randomly distributed on both leaf surfaces, and protruded ca. 1 mm from the leaf surface. Sometimes they were closely aggregated on midrib and leaves, which, consequently, appeared strongly deformed. Close-up observations revealed that gall induction causes hyperplastic proliferation of leaf tissues around the gall chamber hosting mites. The uniserial cell lining inside this chamber provides the nutritional tissue for the mites. All feeding cells contained one or more (frequently 2-3) hypertrophied nuclei and dense granular cytoplasm.", "gemini": "Ve rệp vảy Aceria massalongoi (Canestrini) được thu thập từ các u sưng hình cầu trên cây trinh nữ, Vitex agnus-castus L. (Lamiaceae), bị thương nặng ở Bari và Bernalda (miền nam nước Ý) và trên đảo Ionian Leukade (Hy Lạp). Con cái, con đực và ấu trùng được mô tả chi tiết, theo sơ đồ mô tả hình thái hiện tại, bổ sung các mô tả cũ và không đầy đủ. Đặc điểm phân tử của A. massalongoi từ Ý và Hy Lạp được thực hiện bằng cách khuếch đại và giải trình tự ITS ribosome, miền mở rộng D2-D3 của gen rRNA 28S và COI ty thể, lần đầu tiên. Các cây phát sinh loài dựa trên ba dấu hiệu phân tử cho thấy kết quả nhất quán, xác nhận rằng quần thể A. massalongoi của Ý và Hy Lạp là cùng một loài tụ tập với nhau với một số biến thể trong loài. Các khối u có đường kính từ 0,5 đến 2,8 mm, phân bố ngẫu nhiên trên cả hai bề mặt lá và nhô ra khoảng 1 mm so với bề mặt lá. Đôi khi chúng tập trung chặt chẽ ở gân giữa và lá, do đó, lá xuất hiện bị biến dạng mạnh. Các quan sát cận cảnh cho thấy sự hình thành khối u gây ra sự tăng sinh tăng sản của các mô lá xung quanh buồng khối u chứa ve. Lớp tế bào đơn hàng bên trong buồng này cung cấp mô dinh dưỡng cho ve. Tất cả các tế bào ăn đều chứa một hoặc nhiều (thường là 2-3) nhân phì đại và tế bào chất dạng hạt dày đặc."} +{"idx": 1870620, "en": "BACKGROUND It is necessary to clarify that temporomandibular joint disorders (TMDs) are one of the most misdiagnosed and mistreated maladies in the medical practice. It is an umbrella term, embracing conditions which involve the temporomandibular joint (TMJ) and related muscles. One of these common irritating disorders is the internal derangement which is used specifically to describe the displacement of the TMJ disc. The treatment can vary according to the severity and chronicity into non-invasive, minimally invasive, and invasive procedures. However, permanent recovery is rarely obtained. AIM OF THE STUDY This study was established to compare the effectiveness of two minimally invasive procedures: arthrocentesis and glucocorticosteroid (GCS) local single joint injection in the management of internal derangement of the TMJ. METHODS Thirty patients aged from 18 to 42 years were included in this study with internal derangement which was confirmed clinically and with a cone beam CT scan. The patients were divided into two groups of 15 patients. Arthrocentesis was performed to one group (group A) by using Shepard's cannula and lactated Ringer's solution. Glucocorticosteroid injection was done to the other group (group B) using a 1 ml/40 mg methylprednisolone acetate vial. The study was performed in the Department of Oral and Maxillofacial Surgery, Ghazi Alhareery Hospital-Medical City, from October 2017 to September 2018. RESULTS AND CONCLUSION After 4 months of clinical follow-up, the results revealed that the GCS injection has minimal outcomes in the treatment of TMJ internal derangement compared to arthrocentesis. On the other hand, arthrocentesis and lavage had dedicated promising outcomes.", "gemini": "BỐI CẢNH Cần phải làm rõ rằng rối loạn khớp thái dương hàm (TMD) là một trong những bệnh lý được chẩn đoán sai và điều trị sai nhiều nhất trong thực hành y tế. Đây là một thuật ngữ chung, bao gồm các tình trạng liên quan đến khớp thái dương hàm (TMJ) và các cơ liên quan. Một trong những rối loạn khó chịu phổ biến này là sai lệch bên trong, được sử dụng cụ thể để mô tả sự dịch chuyển của đĩa TMJ. Việc điều trị có thể thay đổi tùy theo mức độ nghiêm trọng và mãn tính thành các thủ thuật không xâm lấn, xâm lấn tối thiểu và xâm lấn. Tuy nhiên, hiếm khi đạt được sự phục hồi vĩnh viễn. MỤC ĐÍCH CỦA NGHIÊN CỨU Nghiên cứu này được thiết lập để so sánh hiệu quả của hai thủ thuật xâm lấn tối thiểu: chọc dò khớp và tiêm một khớp tại chỗ glucocorticosteroid (GCS) trong việc điều trị sai lệch bên trong của TMJ. PHƯƠNG PHÁP Ba mươi bệnh nhân từ 18 đến 42 tuổi được đưa vào nghiên cứu này với tình trạng sai lệch bên trong được xác nhận lâm sàng và bằng chụp cắt lớp chùm tia hình nón. Bệnh nhân được chia thành hai nhóm gồm 15 bệnh nhân. Chọc dò khớp được thực hiện cho một nhóm (nhóm A) bằng cách sử dụng ống thông Shepard và dung dịch Ringer lactat. Tiêm glucocorticosteroid được thực hiện cho nhóm còn lại (nhóm B) bằng cách sử dụng lọ methylprednisolone acetate 1 ml/40 mg. Nghiên cứu được thực hiện tại Khoa Phẫu thuật răng hàm mặt, Bệnh viện Ghazi Alhareery-Thành phố y tế, từ tháng 10 năm 2017 đến tháng 9 năm 2018. KẾT QUẢ VÀ KẾT LUẬN Sau 4 tháng theo dõi lâm sàng, kết quả cho thấy tiêm GCS có kết quả tối thiểu trong điều trị sai lệch bên trong TMJ so với chọc dò khớp. Mặt khác, chọc dò khớp và rửa sạch có kết quả hứa hẹn chuyên dụng."} +{"idx": 1870621, "en": "Currently, no specific drug and vaccine are available for the new coronavirus SARS-CoV-2, and nutritional supplementation should be helpful. This study tried to provide reference for protein supplementation. Specifically, in silico method was employed to simulate protein degradation by gastrointestinal enzymes and to produce a large number of active peptides, then, the binding ability of these peptides to SARS-CoV-2 spike protein receptor-binding domain (RBD) was evaluated. The results showed that wheat-derived alpha/beta-gliadin, oat-derived avenin, and ribulose bisphosphate carboxylase small chain of different origin could be good protein source in generating potent binders to SARS-CoV-2 spike RBD. In addition, some high-affinity oligopeptides (such as PISCR, VQVVN, PQQQF, etc.) were identified as potential binders of SARS-CoV-2 spike RBD. In summary, a number of plant proteins could be helpful for COVID-19 patients when supplemented with these proteins, the identified oligopeptides could be used as lead compound to design potential entry inhibitors against SARS-CoV-2.", "gemini": "Hiện tại, không có loại thuốc và vắc-xin nào được chỉ định cụ thể cho loại vi-rút corona mới SARS-CoV-2, và việc bổ sung dinh dưỡng có thể hữu ích. Nghiên cứu này cố gắng cung cấp tài liệu tham khảo cho việc bổ sung protein. Cụ thể, phương pháp in silico đã được sử dụng để mô phỏng quá trình phân giải protein của các enzym đường tiêu hóa và tạo ra một số lượng lớn các peptide hoạt động, sau đó, khả năng liên kết của các peptide này với miền liên kết thụ thể protein gai SARS-CoV-2 (RBD) được đánh giá. Kết quả cho thấy alpha/beta-gliadin có nguồn gốc từ lúa mì, avenin có nguồn gốc từ yến mạch và chuỗi nhỏ của ribulose bisphosphate carboxylase có nguồn gốc khác nhau có thể là nguồn protein tốt để tạo ra các chất liên kết mạnh với SARS-CoV-2 gai RBD. Ngoài ra, một số oligopeptit có ái lực cao (như PISCR, VQVVN, PQQQF, v.v.) được xác định là chất liên kết tiềm năng của SARS-CoV-2 gai RBD. Tóm lại, một số protein thực vật có thể hữu ích cho bệnh nhân COVID-19 khi được bổ sung các protein này, các oligopeptit được xác định có thể được sử dụng làm hợp chất chính để thiết kế các chất ức chế xâm nhập tiềm năng chống lại SARS-CoV-2."} +{"idx": 1870622, "en": "Advancements in the design and functionality of continuous flow left ventricular assist devices (CF-LVADs), as well as a limited number of donor hearts, have resulted in an increased utilization of this therapy among advanced heart failure (HF) patients. Despite these advancements, gastrointestinal bleeding (GIB) remains a common complication after CF-LVAD implantation. The mechanism of GIB in these patients is complex and includes a combination of angiodysplasia, platelet dysfunction, acquired von Willebrand disease, and a variety of patient-specific factors including advanced age and history of GIB. Several pharmacotherapy options have been reported in the literature, though studies supporting the use of these agents are often small, retrospective reports. Within this review, we discuss the various pharmacologic agents, their proposed mechanisms of action, and the available literature pertaining to their effectiveness and tolerability. Additionally, we propose an evidence-based treatment algorithm, encompassing the updated literature, cost of therapy, medication side effects, and ease of administration.", "gemini": "Những tiến bộ trong thiết kế và chức năng của các thiết bị hỗ trợ tâm thất trái dòng liên tục (CF-LVAD), cũng như số lượng tim hiến tặng hạn chế, đã dẫn đến việc sử dụng liệu pháp này nhiều hơn ở những bệnh nhân suy tim giai đoạn cuối (HF). Mặc dù có những tiến bộ này, xuất huyết tiêu hóa (GIB) vẫn là một biến chứng phổ biến sau khi cấy ghép CF-LVAD. Cơ chế của GIB ở những bệnh nhân này rất phức tạp và bao gồm sự kết hợp của chứng loạn sản mạch máu, rối loạn chức năng tiểu cầu, bệnh von Willebrand mắc phải và nhiều yếu tố đặc hiệu của bệnh nhân bao gồm tuổi cao và tiền sử GIB. Một số lựa chọn điều trị bằng dược lý đã được báo cáo trong tài liệu, mặc dù các nghiên cứu hỗ trợ việc sử dụng các tác nhân này thường là các báo cáo hồi cứu nhỏ. Trong bài đánh giá này, chúng tôi thảo luận về các tác nhân dược lý khác nhau, cơ chế hoạt động được đề xuất của chúng và các tài liệu có sẵn liên quan đến hiệu quả và khả năng dung nạp của chúng. Ngoài ra, chúng tôi đề xuất một thuật toán điều trị dựa trên bằng chứng, bao gồm các tài liệu cập nhật, chi phí điều trị, tác dụng phụ của thuốc và dễ dàng sử dụng."} +{"idx": 1870623, "en": "Current cognitive assessments suffer from limited scalability and high user burden. This study aimed to (1) examine the relationship between a brief eye-tracking-based visual paired-comparison (VPC) and gold standard cognitive assessments, (2) examine longitudinal stability of the VPC task, (3) determine the ability of the VPC task to differentiate between cognitively normal (CN) individuals and individuals with mild cognitive impairment (MCI). Fifty-five adults (n = 44 CN, n = 11 MCI; 56.4 ± 26.7 years) were tested on two occasions, separated by at least 14 days. Visit 1 included VPC, Montreal Cognitive Assessment (MoCA), Digit Symbol Coding test (DSC), and NIH Toolbox Cognitive Battery (NIHTB-CB). Visit 2 included VPC, DSC, NIHTB-CB, and dual-task (DT). Significant differences existed between baseline VPC scores for CN and MCI groups (p < .001). VPC scores remained stable over time in both groups (p < .05). Significant associations existed between VPC and MoCA (p < .01), DSC (p < .001), and various NIHTB-CB subtests at both time points. The VPC test significantly predicts cognitive outcomes (p < .05), with age and VPC being the only significant predictors. Additionally, area under the curve (receiver operator characteristic = 0.80) for VPC scores demonstrated good classification accuracy. VPC reliably predicted cognitive status while remaining stable over time and displayed significant associations with gold standard cognitive assessments. VPC is a less burdensome and more scalable assessment than traditional tests, enabling longitudinal monitoring of cognitive status in resource-limited environments.", "gemini": "Các đánh giá nhận thức hiện tại gặp phải tình trạng khả năng mở rộng hạn chế và gánh nặng cao cho người dùng. Nghiên cứu này nhằm mục đích (1) kiểm tra mối quan hệ giữa so sánh trực quan theo cặp dựa trên theo dõi mắt (VPC) và các đánh giá nhận thức chuẩn vàng, (2) kiểm tra độ ổn định theo thời gian của tác vụ VPC, (3) xác định khả năng của tác vụ VPC để phân biệt giữa các cá nhân nhận thức bình thường (CN) và các cá nhân bị suy giảm nhận thức nhẹ (MCI). Năm mươi lăm người lớn (n = 44 CN, n = 11 MCI; 56,4 ± 26,7 tuổi) đã được thử nghiệm trong hai lần, cách nhau ít nhất 14 ngày. Lần khám 1 bao gồm VPC, Đánh giá nhận thức Montreal (MoCA), bài kiểm tra mã hóa ký hiệu chữ số (DSC) và Bộ công cụ nhận thức NIH (NIHTB-CB). Lần khám 2 bao gồm VPC, DSC, NIHTB-CB và nhiệm vụ kép (DT). Có sự khác biệt đáng kể giữa điểm VPC ban đầu của nhóm CN và MCI (p <0,001). Điểm VPC vẫn ổn định theo thời gian ở cả hai nhóm (p <0,05). Có sự liên quan đáng kể giữa VPC và MoCA (p <0,01), DSC (p <0,001) và các bài kiểm tra phụ NIHTB-CB khác nhau tại cả hai thời điểm. Bài kiểm tra VPC dự đoán đáng kể kết quả nhận thức (p <0,05), trong đó tuổi và VPC là những yếu tố dự báo đáng kể duy nhất. Ngoài ra, diện tích dưới đường cong (đặc tính của người nhận = 0,80) đối với điểm VPC cho thấy độ chính xác phân loại tốt. VPC dự đoán đáng tin cậy tình trạng nhận thức trong khi vẫn ổn định theo thời gian và thể hiện mối liên quan đáng kể với các đánh giá nhận thức chuẩn vàng. VPC là một đánh giá bớt gánh nặng và có khả năng mở rộng hơn các bài kiểm tra truyền thống, cho phép theo dõi tình trạng nhận thức theo thời gian trong các môi trường có nguồn lực hạn chế."} +{"idx": 1870624, "en": "Thrombin is a trypsin-like serine protease with multiple physiological functions. Its role in coagulation and thrombosis is well-established. Nevertheless, thrombin also plays a major role in inflammation by activating protease-activated receptors. In addition, thrombin is also involved in angiogenesis, fibrosis, and viral infections. Considering the pathogenesis of COVID-19 pandemic, thrombin inhibitors may exert multiple potential therapeutic benefits including antithrombotic, anti-inflammatory, and antiviral activities. In this review, we describe the clinical features of COVID-19, the thrombin's roles in various pathologies, and the potential of argatroban in COVID-19 patients. Argatroban is a synthetic, small molecule, direct, competitive, and selective inhibitor of thrombin. It is approved to parenterally prevent and/or treat heparin-induced thrombocytopenia in addition to other thrombotic conditions. Argatroban also possesses anti-inflammatory and antiviral activities and has a well-established pharmacokinetics profile. It also appears to lack a significant risk of drug-drug interactions with therapeutics currently being evaluated for COVID-19. Thus, argatroban presents a substantial promise in treating severe cases of COVID-19; however, this promise is yet to be established in randomized, controlled clinical trials.", "gemini": "Thrombin là một protease serine giống trypsin với nhiều chức năng sinh lý. Vai trò của nó trong quá trình đông máu và huyết khối đã được xác định rõ ràng. Tuy nhiên, thrombin cũng đóng một vai trò chính trong tình trạng viêm bằng cách kích hoạt các thụ thể hoạt hóa protease. Ngoài ra, thrombin cũng tham gia vào quá trình hình thành mạch, xơ hóa và nhiễm trùng do vi-rút. Xem xét đến cơ chế sinh bệnh của đại dịch COVID-19, chất ức chế thrombin có thể phát huy nhiều lợi ích điều trị tiềm năng bao gồm các hoạt động chống huyết khối, chống viêm và kháng vi-rút. Trong bài tổng quan này, chúng tôi mô tả các đặc điểm lâm sàng của COVID-19, vai trò của thrombin trong các bệnh lý khác nhau và tiềm năng của argatroban ở những bệnh nhân mắc COVID-19. Argatroban là một chất ức chế thrombin tổng hợp, phân tử nhỏ, trực tiếp, cạnh tranh và chọn lọc. Nó được chấp thuận để ngăn ngừa và/hoặc điều trị tình trạng giảm tiểu cầu do heparin gây ra ngoài các tình trạng huyết khối khác. Argatroban cũng có hoạt tính chống viêm và kháng vi-rút và có hồ sơ dược động học được xác định rõ ràng. Nó cũng dường như không có nguy cơ tương tác thuốc-thuốc đáng kể với các phương pháp điều trị hiện đang được đánh giá cho COVID-19. Do đó, argatroban thể hiện một triển vọng đáng kể trong việc điều trị các trường hợp COVID-19 nghiêm trọng; tuy nhiên, triển vọng này vẫn chưa được xác định trong các thử nghiệm lâm sàng ngẫu nhiên có đối chứng."} +{"idx": 1870625, "en": "BACKGROUND Faecal incontinence (FI) affects 1-19% of the general population and carries significant physical and psychological morbidity. Treatment strategies vary greatly with respect to morbidity and efficacy and relatively little is known regarding the role of mechanical devices such as anal and vaginal inserts. This is an up-to-date systematic review of the use of these devices in the management of patients with FI. METHODS A systematic electronic search was performed of the Medline, Pubmed and Embase databases using the key words and/or MeSH 'anal plug', 'anal insert', 'vaginal insert' and 'faecal incontinence'. Only articles that reported clinical outcomes for these devices for FI in the English language were included. Review articles were excluded to avoid duplication of data. RESULTS Thirteen articles fulfilled the eligibility criteria. Two articles reported outcomes for the Eclipse vaginal insert and 11 articles reported on three types of anal inserts; the Coloplast 'Tulip' design (6), the Procon/ProTect device (2) and the Renew insert (3). When tolerated, both anal and vaginal inserts significantly improved continence, bowel function and quality of life where reported. Adverse effects included discomfort, leakage and slippage. Long-term compliance and benefit are yet to be determined. CONCLUSIONS Vaginal and anal inserts may be a useful treatment for FI. Better quality of evidence is needed to define its effectiveness.", "gemini": "BỐI CẢNH Tình trạng đại tiện không tự chủ (FI) ảnh hưởng đến 1-19% dân số nói chung và gây ra tình trạng bệnh tật về thể chất và tâm lý đáng kể. Các chiến lược điều trị rất khác nhau về mặt bệnh tật và hiệu quả và tương đối ít được biết đến về vai trò của các thiết bị cơ học như miếng chèn hậu môn và âm đạo. Đây là một bài tổng quan hệ thống cập nhật về việc sử dụng các thiết bị này trong việc quản lý những bệnh nhân mắc FI. PHƯƠNG PHÁP Đã thực hiện tìm kiếm điện tử có hệ thống trên các cơ sở dữ liệu Medline, Pubmed và Embase bằng cách sử dụng các từ khóa và/hoặc MeSH 'nút chặn hậu môn', 'miếng chèn hậu môn', 'miếng chèn âm đạo' và 'đại tiện không tự chủ'. Chỉ những bài báo báo cáo kết quả lâm sàng cho các thiết bị này đối với FI bằng tiếng Anh mới được đưa vào. Các bài báo tổng quan đã bị loại trừ để tránh trùng lặp dữ liệu. KẾT QUẢ Mười ba bài báo đáp ứng các tiêu chí đủ điều kiện. Hai bài báo báo cáo kết quả cho miếng chèn âm đạo Eclipse và 11 bài báo báo cáo về ba loại miếng chèn hậu môn; thiết kế 'Tulip' của Coloplast (6), thiết bị Procon/ProTect (2) và miếng chèn Renew (3). Khi được dung nạp, cả miếng chèn hậu môn và âm đạo đều cải thiện đáng kể khả năng tự chủ, chức năng ruột và chất lượng cuộc sống khi được báo cáo. Các tác dụng phụ bao gồm khó chịu, rò rỉ và trượt. Sự tuân thủ và lợi ích lâu dài vẫn chưa được xác định. KẾT LUẬN Miếng chèn âm đạo và hậu môn có thể là một phương pháp điều trị hữu ích cho FI. Cần có chất lượng bằng chứng tốt hơn để xác định hiệu quả của nó."} +{"idx": 1870626, "en": "Rapid freezing and vitrification using sucrose are two simple and cost-effective sperm cryopreservation methods. However, it is still unclear which method is better and what the optimal concentration of sucrose is. This study aimed to determine the optimal sucrose concentration for human sperm cryopreservation and compare the cryoprotective effects of rapid freezing versus vitrification using different closed straw systems in terms of sperm motility and DNA integrity. Our data showed that: (1) The optimal sucrose concentration for vitrification was 0.25 mol/l among the tested 0, 0.125, 0.25 and 0.5 mol/l concentrations; (2) Sperm total motility and progressive motility were cryopreserved significantly better by rapid freezing than vitrification in standard 0.5 ml cryostraws (P < 0.05); and (3) Sperm total motility and progressive motility were cryopreserved significantly better by vitrification in the straw-in-straw system than rapid freezing in the standard 0.5 cryostraw (P < 0.05), but no difference was found in sperm nuclear DNA fragmentation level between the two cryopreservation methods (P > 0.05). It was concluded that sucrose at 0.25 mol/l concentration is suitable for human sperm rapid freezing and vitrification, and sperm cryopreservation can be achieved by rapid freezing using closed standard 0.5 ml straws or by vitrification using the novel straw-in-straw system made of standard 0.25 and 0.5 ml straws.", "gemini": "Đông lạnh nhanh và thủy tinh hóa bằng sucrose là hai phương pháp bảo quản tinh trùng đơn giản và tiết kiệm chi phí. Tuy nhiên, vẫn chưa rõ phương pháp nào tốt hơn và nồng độ sucrose tối ưu là bao nhiêu. Nghiên cứu này nhằm xác định nồng độ sucrose tối ưu để bảo quản tinh trùng của người và so sánh hiệu quả bảo vệ tế bào của phương pháp đông lạnh nhanh so với phương pháp thủy tinh hóa sử dụng các hệ thống ống kín khác nhau về khả năng vận động của tinh trùng và tính toàn vẹn của DNA. Dữ liệu của chúng tôi cho thấy: (1) Nồng độ sucrose tối ưu để thủy tinh hóa là 0,25 mol/l trong số các nồng độ đã thử nghiệm 0, 0,125, 0,25 và 0,5 mol/l; (2) Khả năng vận động toàn bộ và khả năng vận động tiến triển của tinh trùng được bảo quản bằng phương pháp đông lạnh nhanh tốt hơn đáng kể so với phương pháp thủy tinh hóa trong ống hút trữ lạnh tiêu chuẩn 0,5 ml (P < 0,05); và (3) Khả năng vận động toàn bộ và khả năng vận động tiến triển của tinh trùng được bảo quản bằng phương pháp thủy tinh hóa trong hệ thống ống hút trong ống hút tốt hơn đáng kể so với phương pháp đông lạnh nhanh trong ống hút trữ lạnh tiêu chuẩn 0,5 (P < 0,05), nhưng không có sự khác biệt nào được tìm thấy trong mức độ phân mảnh DNA nhân tinh trùng giữa hai phương pháp bảo quản tinh trùng (P > 0,05). Kết luận rằng sucrose ở nồng độ 0,25 mol/l phù hợp với phương pháp đông lạnh nhanh và thủy tinh hóa tinh trùng của người, và có thể bảo quản tinh trùng bằng phương pháp đông lạnh nhanh bằng ống hút tiêu chuẩn kín 0,5 ml hoặc bằng phương pháp thủy tinh hóa bằng hệ thống ống hút trong ống hút mới được làm bằng ống hút tiêu chuẩn 0,25 và 0,5 ml."} +{"idx": 1870627, "en": "PURPOSE Category 4 in BI-RADS for magnetic resonance imaging (MRI) has a wide range of probabilities of malignancy, extending from > 2 to < 95%. We classified category 4 lesions into three subcategories and analyzed the positive predictive value (PPV) of malignancy in a tertiary hospital. MATERIALS AND METHODS This retrospective study included 346 breast MRIs with 434 category 2-5 lesions. All enhancing lesions were classified as category 2 (0% probability of malignancy), 3 (> 0%, ≤ 2%), 4 (> 2%, < 95%) and 5 (≥ 95%); category 4 lesions were further subcategorized into 4A (> 2%, ≤ 10%), 4B (> 10%, ≤ 50%) and 4C (> 50%, < 95%) at the time of diagnosis. Radiological and pathological reports were retrospectively analyzed, and the PPVs were calculated. RESULTS We included 149 malignant and 285 benign lesions. The PPVs of subcategories 4A, 4B and 4C were 1.8%, 11.8% and 67.5%, respectively. The PPVs were higher for lesions coexisting with category 5 or 6 lesions compared with those for isolated lesions. CONCLUSION Category 4 lesions can be classified into three subcategories depending on the likelihood of malignancy. Lesions coexisting with category 5 or 6 lesions are more likely to be malignant.", "gemini": "MỤC ĐÍCH Thể loại 4 trong BI-RADS đối với hình ảnh cộng hưởng từ (MRI) có một loạt các xác suất ác tính, kéo dài từ> 2 đến <95%. Chúng tôi đã phân loại các tổn thương loại 4 thành ba phân nhóm và phân tích giá trị tiên đoán dương tính (PPV) của ác tính trong một bệnh viện tuyến ba. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu hồi cứu này bao gồm 346 MRI vú với 434 tổn thương loại 2-5. Tất cả các tổn thương tăng cường được phân loại là loại 2 (xác suất ác tính 0%), 3 (> 0%, ≤ 2%), 4 (> 2%, <95%) và 5 (≥ 95%); các tổn thương loại 4 được phân loại phụ thành 4A (> 2%, ≤ 10%), 4B (> 10%, ≤ 50%) và 4C (> 50%, <95%) tại thời điểm chẩn đoán. Các báo cáo về bệnh lý học và hình ảnh học được phân tích hồi cứu và tính toán các PPV. KẾT QUẢ Chúng tôi đã đưa vào 149 tổn thương ác tính và 285 tổn thương lành tính. Các PPV của các phân nhóm 4A, 4B và 4C lần lượt là 1,8%, 11,8% và 67,5%. Các PPV cao hơn đối với các tổn thương cùng tồn tại với các tổn thương loại 5 hoặc 6 so với các tổn thương riêng lẻ. KẾT LUẬN Các tổn thương loại 4 có thể được phân loại thành ba phân nhóm tùy thuộc vào khả năng ác tính. Các tổn thương cùng tồn tại với các tổn thương loại 5 hoặc 6 có nhiều khả năng là ác tính."} +{"idx": 1870628, "en": "Table 2 in the originally published article is not correct and is a duplicate of Table 3. The error happened during typesetting. The correct Table 2 is shown below.", "gemini": "Bảng 2 trong bài báo được xuất bản ban đầu không chính xác và là bản sao của Bảng 3. Lỗi xảy ra trong quá trình sắp chữ. Bảng 2 chính xác được hiển thị bên dưới."} +{"idx": 1870629, "en": "PURPOSE This study aimed to investigate the long-term chronological changes in urination status of patients who underwent radical cystectomy (RC) followed by orthotopic ileal neobladder (ONB) reconstruction using the International Prostatic Symptoms Score (IPSS) and the Overactive Bladder Symptoms Score (OABSS). METHODS This retrospective study focused on patients who underwent RC followed by ONB reconstruction and those who consented for IPSS, quality of life (QOL) based on urinary symptoms (IPSS-QOL), and OABSS assessments in the follow-up period. The patients were divided according to gender into the male group (M-group) and female group (F-group). All patients were evaluated using IPSS, IPSS-QOL, and OABSS every 3 months. The primary endpoint was to assess chronological changes in the urination status of patients who underwent ONB reconstruction after RC. RESULTS The median age of the enrolled patients (n = 122) was 65 years and the median follow-up period was 92.0 months. The median voiding symptom score in IPSS after 10 years of surgery was significantly higher in the M-group than in the F-group. Contrarily, the F-group demonstrated a significantly higher median storage symptom score at 60-66 and 102-114 months than the M-group. The median OABSS scores were relatively higher in the F-group than in the M-group. CONCLUSIONS Although long-term urinary function with ONB demonstrated acceptable results, dysfunctional voiding was observed > 10 years after surgery. Thus, the changes in long-term urinary function should be considered when deciding ONB.", "gemini": "MỤC ĐÍCH Nghiên cứu này nhằm mục đích điều tra những thay đổi theo thời gian dài hạn về tình trạng đi tiểu của những bệnh nhân đã trải qua phẫu thuật cắt bỏ bàng quang gốc (RC) sau đó là tái tạo bàng quang hồi tràng niệu quản (ONB) bằng cách sử dụng Điểm triệu chứng tuyến tiền liệt quốc tế (IPSS) và Điểm triệu chứng bàng quang hoạt động quá mức (OABSS). PHƯƠNG PHÁP Nghiên cứu hồi cứu này tập trung vào những bệnh nhân đã trải qua phẫu thuật RC sau đó là tái tạo ONB và những bệnh nhân đồng ý đánh giá IPSS, chất lượng cuộc sống (QOL) dựa trên các triệu chứng về đường tiết niệu (IPSS-QOL) và OABSS trong thời gian theo dõi. Bệnh nhân được chia theo giới tính thành nhóm nam (nhóm M) và nhóm nữ (nhóm F). Tất cả bệnh nhân được đánh giá bằng IPSS, IPSS-QOL và OABSS cứ 3 tháng một lần. Điểm cuối chính là đánh giá những thay đổi theo thời gian về tình trạng đi tiểu của những bệnh nhân đã trải qua tái tạo ONB sau khi phẫu thuật RC. KẾT QUẢ Độ tuổi trung bình của những bệnh nhân được ghi danh (n = 122) là 65 tuổi và thời gian theo dõi trung bình là 92,0 tháng. Điểm triệu chứng đi tiểu trung bình trong IPSS sau 10 năm phẫu thuật cao hơn đáng kể ở nhóm M so với nhóm F. Ngược lại, nhóm F có điểm triệu chứng dự trữ trung bình cao hơn đáng kể ở tháng thứ 60-66 và 102-114 so với nhóm M. Điểm OABSS trung bình tương đối cao hơn ở nhóm F so với nhóm M. KẾT LUẬN Mặc dù chức năng tiết niệu lâu dài với ONB cho thấy kết quả chấp nhận được, nhưng tình trạng đi tiểu không bình thường được quan sát thấy> 10 năm sau phẫu thuật. Do đó, những thay đổi về chức năng tiết niệu lâu dài nên được xem xét khi quyết định ONB."} +{"idx": 1870630, "en": "PURPOSE The fusion of pre/intraoperative images may improve catheter manipulation during radioembolization (RE) interventions by adding relevant information. The objective of this work is to propose and evaluate the performance of a RE guidance strategy relying on structure-driven intensity-based registration between preoperative CTA and intraoperative X-ray images. METHODS The navigation strategy is decomposed into three image fusion steps, supporting the catheter navigation from the femoral artery till reaching the injection site (IS). During the pretreatment assessment intervention, the aorta and the origins of its side branches are projected on the intraoperative 2D fluoroscopy following a 3D/2D bone-based registration process, to assist the celiac trunk access. Subsequently, a similar approach consisting in projecting the hepatic vasculature on intraoperative DSA through 3D/2D vessel-based registration is performed to assist the IS location. Lastly, the selected IS is reproduced during the treatment intervention by employing 2D/2D image-based registration between pretreatment and treatment fluoroscopic images. RESULTS The three fusion steps were independently evaluated on subsets of 20, 19 and 5 patient cases, respectively. Best results were obtained with gradient difference as similarity measure and with a delimited preoperative vascular structure for vessel-based registration. The approach resulted in qualitatively appropriate anatomical correspondences when projecting the preoperative structures on intraoperative images. With the best configuration, the registration steps showed accuracy and feasibility in aligning data, with global mean landmarks errors of 1.59 mm, 2.32 mm and 2.17 mm, respectively, a computation time that never exceeded 5 s, 25 s and 11 s, respectively, and a user interaction limited to manual initialization of the 3D/2D registration. CONCLUSION An image fusion-based approach has been specifically proposed for RE procedures guidance. The catheter manipulation strategy based on the fusion of pre- and intraoperative images has the potential to support different steps of the RE clinical workflow and to guide the overall procedure.", "gemini": "MỤC ĐÍCH Việc kết hợp hình ảnh trước/trong khi phẫu thuật có thể cải thiện thao tác thông ống trong các can thiệp thuyên tắc mạch máu bằng tia phóng xạ (RE) bằng cách thêm thông tin có liên quan. Mục tiêu của công trình này là đề xuất và đánh giá hiệu suất của một chiến lược hướng dẫn RE dựa trên đăng ký dựa trên cường độ theo cấu trúc giữa CTA trước phẫu thuật và hình ảnh X-quang trong khi phẫu thuật. PHƯƠNG PHÁP Chiến lược dẫn đường được chia thành ba bước kết hợp hình ảnh, hỗ trợ dẫn đường ống thông từ động mạch đùi cho đến khi đến vị trí tiêm (IS). Trong quá trình can thiệp đánh giá tiền điều trị, động mạch chủ và nguồn gốc của các nhánh bên của động mạch chủ được chiếu trên màn huỳnh quang 2D trong khi phẫu thuật sau quy trình đăng ký dựa trên xương 3D/2D, để hỗ trợ tiếp cận thân tạng. Sau đó, một cách tiếp cận tương tự bao gồm chiếu hệ mạch gan trên DSA trong khi phẫu thuật thông qua đăng ký dựa trên mạch 3D/2D được thực hiện để hỗ trợ xác định vị trí IS. Cuối cùng, IS được chọn được tái tạo trong quá trình can thiệp điều trị bằng cách sử dụng đăng ký dựa trên hình ảnh 2D/2D giữa hình ảnh huỳnh quang tiền điều trị và điều trị. KẾT QUẢ Ba bước kết hợp được đánh giá độc lập trên các nhóm phụ gồm 20, 19 và 5 trường hợp bệnh nhân. Kết quả tốt nhất thu được với chênh lệch độ dốc là phép đo độ tương đồng và với cấu trúc mạch máu được xác định trước cho đăng ký dựa trên mạch. Cách tiếp cận này dẫn đến các tương ứng giải phẫu có chất lượng phù hợp khi chiếu các cấu trúc trước phẫu thuật trên hình ảnh trong khi phẫu thuật. Với cấu hình tốt nhất, các bước đăng ký cho thấy độ chính xác và khả thi trong việc căn chỉnh dữ liệu, với lỗi điểm chuẩn trung bình toàn cầu lần lượt là 1,59 mm, 2,32 mm và 2,17 mm, thời gian tính toán không bao giờ vượt quá 5 giây, 25 giây và 11 giây, tương ứng và tương tác của người dùng chỉ giới hạn ở việc khởi tạo thủ công quá trình đăng ký 3D/2D. KẾT LUẬN Một cách tiếp cận dựa trên kết hợp hình ảnh đã được đề xuất cụ thể để hướng dẫn các quy trình RE. Chiến lược thao tác ống thông dựa trên sự kết hợp của hình ảnh trước và trong khi phẫu thuật có khả năng hỗ trợ các bước khác nhau của quy trình làm việc lâm sàng RE và hướng dẫn toàn bộ quy trình."} +{"idx": 1870631, "en": "Malaria is a tropical human disease, caused by protozoan parasites, wherein a significant number of the world's population is at risk. Annually, more than 219 million new cases are reported. Although there are prevention treatments, there are no highly and widely effective licensed anti-malarial vaccines available for use. Opportunities for utilization of plant-based vaccines as novel platforms for developing safe, reliable, and affordable treatments offer promise for developing such a vaccine against malaria. In this study, a Malchloroplast candidate vaccine was designed, composed of segments of AMA1 and MSP1 proteins, two epitopes of Plasmodium falciparum, along with a GK1 peptide from Taenia solium as adjuvant, and this was expressed in tobacco chloroplasts. Transplastomic tobacco lines were generated using biolistic transformation, and these were confirmed to carry the synthetic gene construct. Expression of the synthetic GK1 peptide was confirmed using RT-PCR and Western blots. Furthermore, the GK1 peptide was detected by HPLC at levels of up to 6 µg g-1 dry weight of tobacco leaf tissue. The plant-derived Malchloroplast candidate vaccine was subsequently tested in BALB/c female mice following subcutaneous administration, and was found to elicit specific humoral responses. Furthermore, components of this candidate vaccine were recognized by antibodies in Plasmodium falciparum malaria patients and were immunogenic in test mice. Thus, this study provided a 'proof of concept' for a promising plant-based candidate subunit vaccine against malaria.", "gemini": "Sốt rét là một bệnh nhiệt đới của con người, do ký sinh trùng động vật nguyên sinh gây ra, trong đó một số lượng đáng kể dân số thế giới có nguy cơ mắc bệnh. Hàng năm, có hơn 219 triệu ca mắc mới được báo cáo. Mặc dù có các phương pháp điều trị phòng ngừa, nhưng không có vắc-xin chống sốt rét có hiệu quả cao và được cấp phép rộng rãi nào có sẵn để sử dụng. Các cơ hội sử dụng vắc-xin có nguồn gốc từ thực vật làm nền tảng mới để phát triển các phương pháp điều trị an toàn, đáng tin cậy và giá cả phải chăng hứa hẹn cho việc phát triển một loại vắc-xin như vậy chống lại bệnh sốt rét. Trong nghiên cứu này, một vắc-xin ứng viên Malchloroplast đã được thiết kế, bao gồm các phân đoạn của protein AMA1 và MSP1, hai epitop của Plasmodium falciparum, cùng với một peptit GK1 từ Taenia solium làm tá dược, và điều này được biểu hiện trong lục lạp thuốc lá. Các dòng thuốc lá chuyển gen lục lạp đã được tạo ra bằng cách chuyển đổi sinh học, và những dòng này được xác nhận là mang cấu trúc gen tổng hợp. Sự biểu hiện của peptit GK1 tổng hợp đã được xác nhận bằng RT-PCR và giấy thấm Western. Hơn nữa, peptit GK1 đã được phát hiện bằng HPLC ở mức lên đến 6 µg g-1 trọng lượng khô của mô lá thuốc lá. Vắc-xin ứng viên Malchloroplast có nguồn gốc từ thực vật sau đó đã được thử nghiệm trên chuột cái BALB/c sau khi tiêm dưới da và được phát hiện là gây ra phản ứng dịch thể đặc hiệu. Hơn nữa, các thành phần của vắc-xin ứng viên này đã được các kháng thể ở những bệnh nhân sốt rét Plasmodium falciparum nhận ra và gây miễn dịch ở chuột thử nghiệm. Do đó, nghiên cứu này đã cung cấp một 'bằng chứng về khái niệm' cho một vắc-xin tiểu đơn vị ứng viên có nguồn gốc từ thực vật đầy hứa hẹn chống lại bệnh sốt rét."} +{"idx": 1870632, "en": "PURPOSE OF REVIEW Pulmonary arterial hypertension (PAH) is a progressive disease with high mortality. A greater understanding of the physiology and function of the cardiovascular system in PAH will help improve survival. This review covers the latest advances within cardiovascular magnetic resonance imaging (CMR) regarding diagnosis, evaluation of treatment, and prognostication of patients with PAH. RECENT FINDINGS New CMR measures that have been proven relevant in PAH include measures of ventricular and atrial volumes and function, tissue characterization, pulmonary artery velocities, and arterio-ventricular coupling. CMR markers carry prognostic information relevant for clinical care such as treatment response and thereby can affect survival. Future research should investigate if CMR, as a non-invasive method, can improve existing measures or even provide new and better measures in the diagnosis, evaluation of treatment, and determination of prognosis of PAH.", "gemini": "MỤC ĐÍCH CỦA ĐÁNH GIÁ Tăng huyết áp động mạch phổi (PAH) là một bệnh tiến triển có tỷ lệ tử vong cao. Hiểu biết sâu hơn về sinh lý và chức năng của hệ thống tim mạch trong PAH sẽ giúp cải thiện tỷ lệ sống sót. Đánh giá này bao gồm những tiến bộ mới nhất trong lĩnh vực chụp cộng hưởng từ tim mạch (CMR) liên quan đến chẩn đoán, đánh giá điều trị và tiên lượng của bệnh nhân PAH. NHỮNG PHÁT HIỆN GẦN ĐÂY Các biện pháp CMR mới đã được chứng minh là có liên quan trong PAH bao gồm các biện pháp về thể tích và chức năng của tâm thất và tâm nhĩ, đặc điểm mô, vận tốc động mạch phổi và liên hợp động mạch-tâm thất. Các dấu hiệu CMR mang thông tin tiên lượng có liên quan đến chăm sóc lâm sàng như đáp ứng điều trị và do đó có thể ảnh hưởng đến tỷ lệ sống sót. Nghiên cứu trong tương lai nên điều tra xem CMR, như một phương pháp không xâm lấn, có thể cải thiện các biện pháp hiện có hay thậm chí cung cấp các biện pháp mới và tốt hơn trong chẩn đoán, đánh giá điều trị và xác định tiên lượng của PAH hay không."} +{"idx": 1870633, "en": "PURPOSE OF REVIEW We aim to summarize recent insights and provide an up-to-date overview on the role of intra-aortic balloon pump (IABP) counterpulsation in cardiogenic shock (CS). RECENT FINDINGS In the largest randomized controlled trial (RCT) of patients with CS after acute myocardial infarction (AMICS), IABP did not lower mortality. However, recent data suggest a role for IABP in patients who have persistent ischemia after revascularization. Moreover, in the growing population of CS not caused by acute coronary syndrome (ACS), multiple retrospective studies and one small RCT report on significant hemodynamic improvement following (early) initiation of IABP support, which allowed bridging of most patients to recovery or definitive therapies like heart transplant or a left ventricular assist device (LVAD). Routine use of IABP in patients with AMICS is not recommended, but many patients with CS either from ischemic or non-ischemic cause may benefit from IABP at least for hemodynamic improvement in the short term. There is a need for a larger RCT regarding the role of IABP in selected patients with ACS, as well as in patients with non-ACS CS.", "gemini": "MỤC ĐÍCH CỦA ĐÁNH GIÁ Chúng tôi muốn tóm tắt những hiểu biết gần đây và cung cấp một cái nhìn tổng quan cập nhật về vai trò của phản xung bóng bơm trong động mạch chủ (IABP) trong sốc tim (CS). PHÁT HIỆN GẦN ĐÂY Trong thử nghiệm đối chứng ngẫu nhiên (RCT) lớn nhất ở những bệnh nhân bị CS sau nhồi máu cơ tim cấp (AMICS), IABP không làm giảm tỷ lệ tử vong. Tuy nhiên, dữ liệu gần đây cho thấy vai trò của IABP ở những bệnh nhân vẫn bị thiếu máu cục bộ sau khi tái thông mạch. Hơn nữa, trong nhóm dân số bị CS ngày càng tăng không phải do hội chứng vành cấp (ACS), nhiều nghiên cứu hồi cứu và một RCT nhỏ báo cáo về sự cải thiện huyết động đáng kể sau khi bắt đầu hỗ trợ IABP (sớm), cho phép chuyển hầu hết bệnh nhân đến hồi phục hoặc các liệu pháp dứt điểm như ghép tim hoặc thiết bị hỗ trợ tâm thất trái (LVAD). Không khuyến cáo sử dụng IABP thường quy ở những bệnh nhân bị AMICS, nhưng nhiều bệnh nhân bị CS do nguyên nhân thiếu máu cục bộ hoặc không do thiếu máu cục bộ có thể được hưởng lợi từ IABP ít nhất là để cải thiện huyết động trong thời gian ngắn. Cần có một RCT lớn hơn về vai trò của IABP ở những bệnh nhân được lựa chọn bị ACS, cũng như ở những bệnh nhân bị CS không phải ACS."} +{"idx": 1870634, "en": "Myxofibrosarcoma (MFS) is among the most aggressive and complex sarcoma types that require novel therapeutic approaches for improved clinical outcomes. MFS displays highly complex karyotypes, and frequent alterations in p53 signaling and cell cycle checkpoint genes as well as loss-of-function mutations in NF1 and PTEN have been reported. The effects of radiotherapy and chemotherapy on MFS are limited, and complete surgical resection is the only curative treatment. Thus, the development of novel therapeutic strategies for MFS has long been long desired for MFS. Patient-derived cell lines are an essential tool for basic and translational research in oncology. However, public cell banks provide only a limited number of MFS cell lines. In this study, we aimed to develop a novel patient-derived MFS cell line, which was established from the primary tumor tissue of a 71-year-old male patient with MFS and was named NCC-MFS2-C1. A single-nucleotide polymorphism assay revealed that NCC-MFS2-C1 cells exhibited gain and loss of genetic loci. NCC-MFS2-C1 cells were maintained as a monolayer culture for over 24 passages for 10 months. The cells exhibited spindle-like morphology, continuous growth, and capacity for spheroid formation and invasion. Screening of 213 anticancer agents revealed that bortezomib, gemcitabine, romidepsin, and topotecan at low concentrations inhibited the proliferation of NCC-MFS2-C1 cells. In conclusion, we established a novel MFS cell line, NCC-MFS2-C1, which can be used for studying the molecular mechanisms underlying tumor development and for the in vitro screening of anti-cancer drugs.", "gemini": "Myxofibrosarcoma (MFS) là một trong những loại sarcoma hung dữ và phức tạp nhất, đòi hỏi các phương pháp điều trị mới để cải thiện kết quả lâm sàng. MFS biểu hiện kiểu nhân rất phức tạp và các biến đổi thường xuyên trong tín hiệu p53 và các gen kiểm soát điểm dừng chu kỳ tế bào cũng như đột biến mất chức năng trong NF1 và PTEN đã được báo cáo. Hiệu quả của xạ trị và hóa trị đối với MFS là hạn chế và cắt bỏ hoàn toàn bằng phẫu thuật là phương pháp điều trị duy nhất có thể chữa khỏi. Do đó, từ lâu người ta đã mong muốn phát triển các chiến lược điều trị mới cho MFS. Các dòng tế bào có nguồn gốc từ bệnh nhân là một công cụ thiết yếu cho nghiên cứu cơ bản và chuyển dịch trong ung thư học. Tuy nhiên, các ngân hàng tế bào công cộng chỉ cung cấp một số lượng hạn chế các dòng tế bào MFS. Trong nghiên cứu này, chúng tôi nhằm mục đích phát triển một dòng tế bào MFS mới có nguồn gốc từ bệnh nhân, được thiết lập từ mô khối u nguyên phát của một bệnh nhân nam 71 tuổi bị MFS và được đặt tên là NCC-MFS2-C1. Xét nghiệm đa hình một nucleotide cho thấy các tế bào NCC-MFS2-C1 biểu hiện tình trạng tăng và mất các locus di truyền. Các tế bào NCC-MFS2-C1 được duy trì dưới dạng nuôi cấy đơn lớp trong hơn 24 lần truyền trong 10 tháng. Các tế bào biểu hiện hình thái giống trục lưỡng cực, tăng trưởng liên tục và khả năng hình thành hình cầu và xâm lấn. Việc sàng lọc 213 tác nhân chống ung thư cho thấy bortezomib, gemcitabine, romidepsin và topotecan ở nồng độ thấp ức chế sự tăng sinh của các tế bào NCC-MFS2-C1. Tóm lại, chúng tôi đã thiết lập một dòng tế bào MFS mới, NCC-MFS2-C1, có thể được sử dụng để nghiên cứu các cơ chế phân tử cơ bản sự phát triển khối u và để sàng lọc các loại thuốc chống ung thư trong ống nghiệm."} +{"idx": 1870635, "en": "Conventional influenza vaccines are based on predicting the circulating viruses year by year, conferring limited effectiveness since the antigenicity of vaccine strains does not always match the circulating viruses. This necessitates development of universal influenza vaccines that provide broader and lasting protection against pan-influenza viruses. The discovery of the highly conserved immunogens (epitopes) of influenza viruses provides attractive targets for universal vaccine design. Here we review the current understanding with broadly protective immunogens (epitopes) and discuss several important considerations to achieve the goal of universal influenza vaccines.", "gemini": "Vắc-xin cúm thông thường dựa trên việc dự đoán các loại vi-rút lưu hành từng năm, mang lại hiệu quả hạn chế vì tính kháng nguyên của các chủng vắc-xin không phải lúc nào cũng phù hợp với các loại vi-rút lưu hành. Điều này đòi hỏi phải phát triển các vắc-xin cúm phổ quát cung cấp khả năng bảo vệ rộng hơn và lâu dài hơn chống lại các loại vi-rút cúm toàn phần. Việc phát hiện ra các yếu tố miễn dịch (epitop) được bảo tồn cao của vi-rút cúm cung cấp các mục tiêu hấp dẫn cho thiết kế vắc-xin phổ quát. Tại đây, chúng tôi xem xét lại sự hiểu biết hiện tại với các yếu tố miễn dịch bảo vệ rộng rãi (epitop) và thảo luận về một số cân nhắc quan trọng để đạt được mục tiêu của vắc-xin cúm phổ quát."} +{"idx": 1870636, "en": "Label-free characterization of cell subpopulations is a very promising biomedical approach. Nowadays, there are several label-free methods based on different physical properties such as size, density, stiffness, etc. allowing the characterization of biological objects. However, fluorescence properties are the most suitable feature for the label-free study of tissue and cells. Understanding the autofluorescence level peculiarities of normal and pathological / live and dead cells can become a helpful tool for cells' metabolic activity, viability evaluation, and diagnostics of a number of diseases. In this study, we applied a series of mouse cell lines (RAW 264.7 - macrophages, L929 - fibroblasts, C2C12 - myoblasts, and B16-F10 - melanoma) to compare cell autofluorescence of live and dead cells under 488 nm laser excitation and found the difference between their autofluorescence depending on a cell state and type.", "gemini": "Đặc điểm không nhãn của các phân nhóm tế bào là một phương pháp tiếp cận y sinh rất hứa hẹn. Ngày nay, có một số phương pháp không nhãn dựa trên các tính chất vật lý khác nhau như kích thước, mật độ, độ cứng, v.v. cho phép đặc trưng các đối tượng sinh học. Tuy nhiên, các tính chất huỳnh quang là đặc điểm phù hợp nhất cho nghiên cứu không nhãn về mô và tế bào. Việc hiểu được những đặc điểm về mức độ tự huỳnh quang của các tế bào bình thường và bệnh lý / sống và chết có thể trở thành một công cụ hữu ích để đánh giá hoạt động trao đổi chất của tế bào, khả năng sống và chẩn đoán một số bệnh. Trong nghiên cứu này, chúng tôi đã áp dụng một loạt các dòng tế bào chuột (RAW 264.7 - đại thực bào, L929 - nguyên bào sợi, C2C12 - nguyên bào cơ và B16-F10 - tế bào u hắc tố) để so sánh tự huỳnh quang của tế bào sống và tế bào chết dưới sự kích thích laser 488 nm và phát hiện ra sự khác biệt giữa tự huỳnh quang của chúng tùy thuộc vào trạng thái và loại tế bào."} +{"idx": 1870637, "en": "In this study, two metal Schiff base complexes (cobalt(II) and manganese(II), were synthesized and characterized by 1H-NMR and FT-IR analyses. The in vitro binding behavior of this complexes with β-Casein (β-CN) was investigated by using biophysical techniques. For evaluation the thermodynamics parameters of interaction between Schiff base complexes and β-CN,the fluorescence data at different temperatures were done. The results showed that the intrinsic fluorescence of the β-CN was quenched by increasing the complexes through the dynamic quenching mechanism. Also, these complexes demonstrated a considrable binding affinity to β-CN and the process is mainly entropy driven. Fluorescence resonance energy transfer was used in order to estimating the distance between donor (β-CN) and acceptor (complex). Results demonstrated that the sequence of tendency of the complexes to β-CN was as follows: Mn-Schiff base complex > Co-Schiff base complex. All these experimental results propose that β-CN might act as carrier protein for Co(II) and Mn (II) complexes to deliver it to the target molecules.", "gemini": "Trong nghiên cứu này, hai hợp chất phức bazơ Schiff kim loại (coban (II) và mangan (II), đã được tổng hợp và đặc trưng bằng các phép phân tích 1H-NMR và FT-IR. Hành vi liên kết trong ống nghiệm của các hợp chất phức này với β-Casein (β-CN) đã được nghiên cứu bằng cách sử dụng các kỹ thuật sinh lý học. Để đánh giá các thông số nhiệt động lực học của tương tác giữa các hợp chất phức bazơ Schiff và β-CN, dữ liệu huỳnh quang ở các nhiệt độ khác nhau đã được thực hiện. Kết quả cho thấy huỳnh quang nội tại của β-CN bị dập tắt khi tăng các hợp chất phức thông qua cơ chế dập tắt động. Ngoài ra, các hợp chất phức này đã chứng minh được ái lực liên kết đáng kể đối với β-CN và quá trình này chủ yếu do entropy thúc đẩy. Chuyển năng lượng cộng hưởng huỳnh quang được sử dụng để ước tính khoảng cách giữa chất cho (β-CN) và chất nhận (hợp chất phức). Kết quả chứng minh rằng trình tự xu hướng của các hợp chất phức đối với β-CN như sau: Hợp chất phức bazơ Schiff Mn> Hợp chất phức bazơ Schiff Co. Tất cả các kết quả thực nghiệm này đều đề xuất rằng β-CN có thể hoạt động như protein vận chuyển cho các hợp chất phức Co (II) và Mn (II) để đưa chúng đến các phân tử đích."} +{"idx": 1870638, "en": "Cysteine (Cys) is an important endogenous amino acid and plays critical physiological roles in living systems. Herein, an endoplasmic reticulum (ER)-targeting fluorescent probe (FER-Cys) was designed and prepared for imaging of Cys in living cells. The probe FER-Cys consists of a fluorescein framework as the fluorescent platform, acrylate group as the response site for the selective recognition of Cys, and ER-specific p-toluenesulfonamide fragment. After the response of probe FER-Cys to Cys, a turn-on fluorescence signal at 546 nm could be detected obviously. The probe FER-Cys further shows desirable selectivity to Cys. Finally, the probe FER-Cys was proven to selectively detect Cys in live cells and successfully image the changes of Cys level in the cell models of H2O2-induced redox imbalance.", "gemini": "Cystein (Cys) là một axit amin nội sinh quan trọng và đóng vai trò sinh lý quan trọng trong các hệ thống sống. Tại đây, một đầu dò huỳnh quang nhắm vào lưới nội chất (ER) (FER-Cys) đã được thiết kế và chế tạo để chụp ảnh Cys trong các tế bào sống. Đầu dò FER-Cys bao gồm một khung fluorescein làm nền tảng huỳnh quang, nhóm acrylate làm vị trí đáp ứng để nhận biết chọn lọc Cys và đoạn p-toluenesulfonamide đặc hiệu của ER. Sau phản ứng của đầu dò FER-Cys với Cys, có thể phát hiện rõ ràng tín hiệu huỳnh quang bật ở 546 nm. Đầu dò FER-Cys tiếp tục thể hiện tính chọn lọc mong muốn đối với Cys. Cuối cùng, đầu dò FER-Cys đã được chứng minh là có thể phát hiện chọn lọc Cys trong các tế bào sống và chụp ảnh thành công sự thay đổi của mức Cys trong các mô hình tế bào mất cân bằng oxy hóa do H2O2 gây ra."} +{"idx": 1870639, "en": "The synthesis of 1-benzyl-2-((2-Aminoethyl) amino)-5-oxopyrrolidine-3,4-diyl diacetate (boad), an oxopyrrolidine type ligand; designed to coordinate lanthanides (Eu3+ and Tb3+) to get luminescent material. The target complexes showed good photoluminescence properties, which indicate that this type of compound can be used as sensitizers having luminescence for the green (Tb3+) and red (Eu3+) emission. The obtained results revealed that sensitizer efficiency can be improved by adding ligands like acac (Eu(acac)3, which has also enhanced the luminescence quantum output and period for Eu3+ ions. The ground state geometries were developed by using density functional theory at B3LYP/6-31G** level. The charge transfer analysis and electronic properties were performed. The Europium and Terbium complexes formation with boad ligand was explored based on molecular electrostatic potential, MDC-q charges, and frontier molecular orbitals (FMOs) analysis.", "gemini": "Tổng hợp 1-benzyl-2-((2-Aminoethyl) amino)-5-oxopyrrolidine-3,4-diyl diacetate (boad), một loại phối tử oxopyrrolidine; được thiết kế để phối hợp với các lanthanide (Eu3+ và Tb3+) để tạo ra vật liệu phát quang. Các phức hợp mục tiêu cho thấy các tính chất phát quang tốt, điều này chỉ ra rằng loại hợp chất này có thể được sử dụng làm chất nhạy quang có phát quang cho phát xạ màu xanh lá cây (Tb3+) và màu đỏ (Eu3+). Các kết quả thu được cho thấy hiệu suất của chất nhạy quang có thể được cải thiện bằng cách thêm các phối tử như acac (Eu(acac)3, chất này cũng làm tăng cường lượng tử phát quang và chu kỳ cho các ion Eu3+. Các hình học trạng thái cơ bản được phát triển bằng cách sử dụng lý thuyết hàm mật độ ở mức B3LYP/6-31G**. Phân tích chuyển điện tích và các tính chất điện tử đã được thực hiện. Sự hình thành các phức hợp Europium và Terbium với phối tử boad đã được khám phá dựa trên thế điện tĩnh phân tử, điện tích MDC-q và phân tích các quỹ đạo phân tử biên (FMO)."} +{"idx": 1870640, "en": "Two sets of mononuclear N, O-chelate complexes, 1a [Co(L1)2(H2O)2], 1b [Ni(L1)2] and 1c [Cu(L1)2], and 2a [Co(L2)2(H2O)2], 2b [Ni(L2)2] and 2c [Cu(L2)2], from respective Schiff base ligands, HL1: 2-((E)-(6-(trifluoromethoxy)phenylimino)methyl)-5-methylphenol, C15H12F3NO2) and HL2: 2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4,6-dichlorophenol, C14H8Cl2F3NO2) have been synthesized and well-characterized by analytical and various spectroscopic techniques like mass spectrometry, FT-IR, UV-visible, magnetic susceptibility, ESR and thermogravimetric studies. The geometries are proposed as octahedral for complexes 1a and 2a, whereas square planar for complexes 1b, 1c, and 2b, 2c. DNA binding study was investigated against calf thymus DNA for synthesized complexes comparing with parent ligands and resulted in intercalation mode of binding which was further supported by fluorescence quenching and viscometric titrations. Nuclease efficacy was also investigated against supercoiled pBR322 DNA and a good cleavage pattern was shown by metal complexes. The complexes were found to possess good antimicrobial activity compared to free ligands against bacterial species, Bacillus amyloliquefaciens and Escherichia coli as well as fungal species, Macrophomina phaseolina and Sclerotium rolfsii.", "gemini": "Hai bộ phức hợp chelat N, O đơn nhân, 1a [Co(L1)2(H2O)2], 1b [Ni(L1)2] và 1c [Cu(L1)2], và 2a [Co(L2)2(H2O)2], 2b [Ni(L2)2] và 2c [Cu(L2)2], từ các phối tử bazơ Schiff tương ứng, HL1: 2-((E)-(6-(trifluoromethoxy)phenylimino)methyl)-5-methylphenol, C15H12F3NO2) và HL2: 2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4,6-dichlorophenol, C14H8Cl2F3NO2) đã được tổng hợp và đặc tính hóa tốt bằng các kỹ thuật phân tích và nhiều kỹ thuật quang phổ như phổ khối, FT-IR, UV-VIS, độ nhạy từ, ESR và nghiên cứu nhiệt trọng. Các hình học được đề xuất là hình bát diện cho các phức hợp 1a và 2a, trong khi hình vuông phẳng cho các phức hợp 1b, 1c và 2b, 2c. Nghiên cứu liên kết DNA đã được điều tra đối với DNA tuyến ức bê đối với các phức hợp tổng hợp so sánh với các phối tử gốc và dẫn đến chế độ liên kết xen kẽ, được hỗ trợ thêm bằng sự dập tắt huỳnh quang và chuẩn độ độ nhớt. Hiệu quả của nuclease cũng đã được điều tra đối với DNA pBR322 siêu xoắn và một mẫu phân cắt tốt đã được thể hiện bởi các phức hợp kim loại. Các phức hợp được phát hiện có hoạt tính kháng khuẩn tốt so với các phối tử tự do đối với các loài vi khuẩn, Bacillus amyloliquefaciens và Escherichia coli cũng như các loài nấm, Macrophomina phaseolina và Sclerotium rolfsii."} +{"idx": 1870641, "en": "OBJECTIVE Oxidation contributes to secondary brain injury after spontaneous intracerebral haemorrhage (SIH). One study found lower levels of total antioxidant capacity (TAC) in the blood in patients with SIH than in healthy subjects. However, there are no data on blood TAC levels and survival in patients with SIH. Therefore, the objective of our study was to determine if an association exists between serum TAC levels and mortality in patients with SIH. METHODS We included patients with severe supratentorial SIH. We considered severe when Glasgow Coma Scale (GCS) < 9. Patients from 6 Spanish hospitals were included in this observational and prospective study. Serum TAC levels at days 1, 4 and 8 of SIH were determined. Thirty-day mortality was our end-point study. RESULTS Non-surviving patients compared with surviving patients showed higher serum TAC levels at day 1 (p < 0.001), 4 (p < 0.001) and 8 (p = 0.001). An area under the curve was found for the prediction of 30-day mortality by serum TAC levels of 0.92 (95% CI = 0.85-96%; p < 0.001). Multiple logistic regression analysis showed an association of serum TAC levels with 30-day mortality (odds ratio = 16.513; 95% CI = 2.548-107.015; p = 0.003) controlling for midline shift, glycemia, early evacuation of SIH, intracerebral haemorrhage (ICH) score, age and volume of SIH. CONCLUSIONS The new findings of this study are that serum TAC levels are higher in non-surviving than in surviving patients, and that they are associated with mortality and could be used to predict mortality.", "gemini": "MỤC TIÊU Oxy hóa góp phần gây tổn thương não thứ cấp sau xuất huyết não tự phát (SIH). Một nghiên cứu phát hiện ra rằng nồng độ tổng khả năng chống oxy hóa (TAC) trong máu ở những bệnh nhân bị SIH thấp hơn so với những người khỏe mạnh. Tuy nhiên, không có dữ liệu về nồng độ TAC trong máu và tỷ lệ sống sót ở những bệnh nhân bị SIH. Do đó, mục tiêu của nghiên cứu của chúng tôi là xác định xem có sự liên quan giữa nồng độ TAC trong huyết thanh và tỷ lệ tử vong ở những bệnh nhân bị SIH hay không. PHƯƠNG PHÁP Chúng tôi đã đưa những bệnh nhân bị SIH trên lều nghiêm trọng vào nghiên cứu. Chúng tôi coi là nghiêm trọng khi Điểm hôn mê Glasgow (GCS) < 9. Bệnh nhân từ 6 bệnh viện Tây Ban Nha đã được đưa vào nghiên cứu quan sát và triển vọng này. Nồng độ TAC trong huyết thanh vào các ngày 1, 4 và 8 của SIH đã được xác định. Tỷ lệ tử vong trong 30 ngày là điểm cuối nghiên cứu của chúng tôi. KẾT QUẢ Những bệnh nhân không sống sót so với những bệnh nhân sống sót cho thấy nồng độ TAC trong huyết thanh cao hơn vào ngày 1 (p < 0,001), 4 (p < 0,001) và 8 (p = 0,001). Một diện tích dưới đường cong đã được tìm thấy để dự đoán tỷ lệ tử vong trong 30 ngày theo nồng độ TAC trong huyết thanh là 0,92 (95% CI = 0,85-96%; p < 0,001). Phân tích hồi quy logistic đa biến cho thấy có mối liên quan giữa nồng độ TAC trong huyết thanh với tỷ lệ tử vong trong 30 ngày (tỷ lệ nguy cơ = 16,513; 95% CI = 2,548-107,015; p = 0,003) kiểm soát độ lệch đường giữa, đường huyết, dẫn lưu sớm SIH, điểm xuất huyết não (ICH), tuổi và thể tích SIH. KẾT LUẬN Những phát hiện mới của nghiên cứu này là nồng độ TAC trong huyết thanh cao hơn ở những bệnh nhân không sống sót so với những bệnh nhân sống sót và chúng có liên quan đến tỷ lệ tử vong và có thể được sử dụng để dự đoán tỷ lệ tử vong."} +{"idx": 1870642, "en": "BACKGROUND To investigate whether modifiable predictors (depressive symptoms, impairment in behavior and mood, balance impairments, and knee extensor muscle strength) are determinants of the physical activity level in Parkinson's disease. MATERIALS AND METHODS A cross-sectional study with individuals diagnosed with idiopathic Parkinson's disease. Regression analysis of the data was used to investigate whether depressive symptoms, impairments in behavior and mood, balance impairments, or dominant knee extensor muscle strength are predictors of physical activity levels in Parkinson's disease. RESULTS A total of 50 individuals with mild to moderate Parkinson's disease participated in this study, with a mean age of 67 ± 8 years and 68% male. Balance impairments explained 29% of the variation in the physical activity levels. The explained variance increased to 34% when depressive symptoms were included in the model. CONCLUSION Among the predictor variables investigated in our study, only balance impairments and depressive symptoms explained the variance in physical activity levels in individuals with Parkinson's disease.", "gemini": "BỐI CẢNH Để tìm hiểu liệu các yếu tố dự báo có thể thay đổi (triệu chứng trầm cảm, suy giảm hành vi và tâm trạng, suy giảm thăng bằng và sức mạnh cơ duỗi đầu gối) có phải là yếu tố quyết định mức độ hoạt động thể chất ở bệnh Parkinson hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Một nghiên cứu cắt ngang với những cá nhân được chẩn đoán mắc bệnh Parkinson vô căn. Phân tích hồi quy dữ liệu được sử dụng để tìm hiểu xem các triệu chứng trầm cảm, suy giảm hành vi và tâm trạng, suy giảm thăng bằng hay sức mạnh cơ duỗi đầu gối có phải là yếu tố dự báo mức độ hoạt động thể chất ở bệnh Parkinson hay không. KẾT QUẢ Tổng cộng có 50 cá nhân mắc bệnh Parkinson từ nhẹ đến trung bình tham gia nghiên cứu này, với độ tuổi trung bình là 67 ± 8 tuổi và 68% là nam. Suy giảm thăng bằng giải thích 29% sự thay đổi về mức độ hoạt động thể chất. Phương sai được giải thích tăng lên 34% khi các triệu chứng trầm cảm được đưa vào mô hình. KẾT LUẬN Trong số các biến dự báo được điều tra trong nghiên cứu của chúng tôi, chỉ có suy giảm thăng bằng và các triệu chứng trầm cảm mới giải thích được sự thay đổi về mức độ hoạt động thể chất ở những người mắc bệnh Parkinson."} +{"idx": 1870643, "en": "BACKGROUND Uncertain ascertainment of events in clinical trials has been noted for decades. To correct possible bias, Clinical Endpoint Committees (CECs) have been employed as a critical element of trials to ensure consistent and high-quality endpoint evaluation, especially for cardiovascular endpoints. However, the efficiency and usefulness of adjudication have been debated. METHODS The multiple imputation (MI) method was proposed to incorporate endpoint event uncertainty. In a simulation conducted to explain this methodology, the dichotomous outcome was imputed each time with subject-specific event probabilities. As the final step, the desired analysis was conducted based on all imputed data. This proposed method was further applied to real trial data from PARADIGM-HF. RESULTS Compared with the conventional Cox model with adjudicated events only, the Cox MI method had higher power, even with a small number of uncertain events. It yielded more robust inferences regarding treatment effects and required a smaller sample size to achieve the same power. CONCLUSIONS Instead of using dichotomous endpoint data, the MI method enables incorporation of event uncertainty and eliminates the need for categorizing endpoint events. In future trials, assigning a probability of event occurrence for each event may be preferable to a CEC assigning a dichotomous outcome. Considerable resources could be saved if endpoint events can be identified more simply and in a manner that maintains study power.", "gemini": "BỐI CẢNH Việc xác định không chắc chắn các sự kiện trong các thử nghiệm lâm sàng đã được ghi nhận trong nhiều thập kỷ. Để khắc phục sự thiên lệch có thể xảy ra, các Ủy ban Điểm cuối Lâm sàng (CEC) đã được sử dụng như một yếu tố quan trọng của các thử nghiệm để đảm bảo đánh giá điểm cuối nhất quán và chất lượng cao, đặc biệt là đối với các điểm cuối tim mạch. Tuy nhiên, hiệu quả và tính hữu ích của việc xét xử đã được tranh luận. PHƯƠNG PHÁP Phương pháp bù trừ nhiều lần (MI) được đề xuất để kết hợp sự không chắc chắn về sự kiện điểm cuối. Trong một mô phỏng được tiến hành để giải thích phương pháp luận này, kết quả nhị phân đã được tính toán mỗi lần với xác suất sự kiện cụ thể của đối tượng. Là bước cuối cùng, phân tích mong muốn đã được tiến hành dựa trên tất cả dữ liệu được tính toán. Phương pháp được đề xuất này được áp dụng thêm vào dữ liệu thử nghiệm thực tế từ PARADIGM-HF. KẾT QUẢ So với mô hình Cox thông thường chỉ có các sự kiện được xét xử, phương pháp Cox MI có sức mạnh cao hơn, ngay cả khi có một số lượng nhỏ các sự kiện không chắc chắn. Nó đưa ra những suy luận mạnh mẽ hơn về hiệu quả điều trị và yêu cầu kích thước mẫu nhỏ hơn để đạt được cùng một sức mạnh. KẾT LUẬN Thay vì sử dụng dữ liệu điểm cuối nhị phân, phương pháp MI cho phép kết hợp sự không chắc chắn về sự kiện và loại bỏ nhu cầu phân loại các sự kiện điểm cuối. Trong các thử nghiệm trong tương lai, việc chỉ định xác suất xảy ra sự kiện cho mỗi sự kiện có thể được ưu tiên hơn so với việc CEC chỉ định kết quả nhị phân. Có thể tiết kiệm được nhiều nguồn lực đáng kể nếu các sự kiện điểm cuối có thể được xác định đơn giản hơn và theo cách duy trì sức mạnh nghiên cứu."} +{"idx": 1870644, "en": "PURPOSE To illustrate the change in emergency department (ED) imaging utilization at a multicenter health system in the state of Ohio during the COVID-19 pandemic. METHODS A retrospective observational study was conducted assessing ED imaging volumes between March 1, 2020, and May 11, 2020, during the COVID-19 crisis. A rolling 7-day total value was used for volume tracking and comparison. Total imaging utilization in the ED was compared with new COVID-19 cases in our region. Utilization was first categorized by modality and then by plain films and computed tomography (CT) scans grouped by body part. CT imaging of the chest was specifically investigated by assessing both CT chest only exams and CT chest, abdomen, and pelvis (C/A/P) exams. Ultimately, matching pair-wise statistical analysis of exam volumes was performed to assess significance of volume change. RESULTS Our multicenter health system experienced a 46% drop in imaging utilization (p < 0.0001) during the pandemic. Matching pair-wise analysis showed a statistically significant volume decrease by each modality and body part. The exceptions were non-contrast chest CT, which increased (p = 0.0053), and non-trauma C/A/P CT, which did not show a statistically significant volume change (p = 0.0633). CONCLUSION ED imaging utilization trends revealed through actual health system data will help inform evidence-based decisions for more accurate volume predictions and therefore institutional preparedness for current and future pandemics.", "gemini": "MỤC ĐÍCH Minh họa sự thay đổi trong việc sử dụng hình ảnh tại khoa cấp cứu (ED) tại một hệ thống y tế đa trung tâm ở tiểu bang Ohio trong thời gian xảy ra đại dịch COVID-19. PHƯƠNG PHÁP Một nghiên cứu quan sát hồi cứu đã được tiến hành để đánh giá khối lượng hình ảnh của ED trong khoảng thời gian từ ngày 1 tháng 3 năm 2020 đến ngày 11 tháng 5 năm 2020, trong cuộc khủng hoảng COVID-19. Tổng giá trị lăn trong 7 ngày đã được sử dụng để theo dõi và so sánh khối lượng. Tổng lượng hình ảnh sử dụng trong ED được so sánh với các trường hợp COVID-19 mới trong khu vực của chúng tôi. Việc sử dụng đầu tiên được phân loại theo phương thức và sau đó theo phim thường và chụp cắt lớp vi tính (CT) được nhóm theo bộ phận cơ thể. Chụp CT hình ảnh ngực được điều tra cụ thể bằng cách đánh giá cả hai kỳ thi CT ngực và kỳ thi CT ngực, bụng và khung chậu (C/A/P). Cuối cùng, phân tích thống kê theo cặp phù hợp của khối lượng kiểm tra đã được thực hiện để đánh giá ý nghĩa của sự thay đổi khối lượng. KẾT QUẢ Hệ thống y tế đa trung tâm của chúng tôi đã giảm 46% lượng hình ảnh sử dụng (p < 0,0001) trong thời gian xảy ra đại dịch. Phân tích từng cặp phù hợp cho thấy khối lượng giảm đáng kể về mặt thống kê theo từng phương thức và bộ phận cơ thể. Các trường hợp ngoại lệ là CT ngực không cản quang, tăng (p = 0,0053) và CT C/A/P không chấn thương, không cho thấy sự thay đổi khối lượng có ý nghĩa thống kê (p = 0,0633). KẾT LUẬN Các xu hướng sử dụng hình ảnh của ED được tiết lộ thông qua dữ liệu hệ thống y tế thực tế sẽ giúp đưa ra thông tin quyết định dựa trên bằng chứng để dự đoán khối lượng chính xác hơn và do đó, sự chuẩn bị của tổ chức cho các đại dịch hiện tại và tương lai."} +{"idx": 1870645, "en": "PURPOSE OF REVIEW The aims of this narrative review were to (1) synthesise the literature on the relationship between screen time and important mental health outcomes and (2) examine the underpinning factors that can influence this association. RECENT FINDINGS Paralleling the rise of mental health issues in children and adolescents is the ubiquitous overuse of screens, but it is unclear how screen time is related to important mental health outcomes and whether this association differs by gender, age and screen type. METHODS Medline/PubMed, PsychINFO and Google Scholar databases were searched on December 2019 for articles published mainly in the last 5 years. The search focused on two main concepts: (i) screen time and (ii) mental health outcomes including anxiety, depression, psychological and psychosocial well-being and body image concerns. RESULTS Sixty studies were included in the review. Higher levels of screen time were associated with more severe depressive symptoms. We found moderate evidence for an association between screen time and poor psychological well-being and body dissatisfaction especially among females. Relationships between screen time and anxiety were inconsistent and somewhat gender specific. Social media use was consistently associated with poorer mental health. Higher levels of screen time are generally associated with poorer mental health outcomes, but associations are influenced by screen type, gender and age. Practitioners, parents, policy makers and researchers should collectively identify and evaluate strategies to reduce screen time, or to use screens more adaptively, as a means of promoting better mental health among children and adolescents.", "gemini": "MỤC ĐÍCH CỦA BÀI ĐÁNH GIÁ Mục đích của bài đánh giá tường thuật này là (1) tổng hợp các tài liệu về mối quan hệ giữa thời gian sử dụng màn hình và các kết quả sức khỏe tâm thần quan trọng và (2) xem xét các yếu tố cơ bản có thể ảnh hưởng đến mối liên quan này. PHÁT HIỆN GẦN ĐÂY Song song với sự gia tăng các vấn đề sức khỏe tâm thần ở trẻ em và thanh thiếu niên là tình trạng sử dụng màn hình quá mức ở khắp mọi nơi, nhưng không rõ thời gian sử dụng màn hình liên quan như thế nào đến các kết quả sức khỏe tâm thần quan trọng và liệu mối liên quan này có khác nhau tùy theo giới tính, độ tuổi và loại màn hình hay không. PHƯƠNG PHÁP Các cơ sở dữ liệu Medline/PubMed, PsychINFO và Google Scholar đã được tìm kiếm vào tháng 12 năm 2019 đối với các bài báo chủ yếu được xuất bản trong 5 năm gần đây. Cuộc tìm kiếm tập trung vào hai khái niệm chính: (i) thời gian sử dụng màn hình và (ii) các kết quả sức khỏe tâm thần bao gồm lo lắng, trầm cảm, sức khỏe tâm lý và sức khỏe tâm xã hội và các mối quan tâm về hình ảnh cơ thể. KẾT QUẢ Sáu mươi nghiên cứu đã được đưa vào đánh giá. Thời gian sử dụng màn hình càng cao thì các triệu chứng trầm cảm càng nghiêm trọng. Chúng tôi tìm thấy bằng chứng ở mức độ vừa phải về mối liên quan giữa thời gian sử dụng màn hình và sức khỏe tâm lý kém và không hài lòng về cơ thể, đặc biệt là ở phụ nữ. Mối quan hệ giữa thời gian sử dụng màn hình và lo lắng không nhất quán và có phần phụ thuộc vào giới tính. Việc sử dụng phương tiện truyền thông xã hội luôn đi kèm với sức khỏe tâm thần kém hơn. Thời gian sử dụng màn hình càng cao thì nhìn chung sức khỏe tâm thần càng kém, nhưng mối liên quan này chịu ảnh hưởng của loại màn hình, giới tính và độ tuổi. Các bác sĩ lâm sàng, phụ huynh, nhà hoạch định chính sách và các nhà nghiên cứu nên cùng nhau xác định và đánh giá các chiến lược để giảm thời gian sử dụng màn hình hoặc sử dụng màn hình một cách thích ứng hơn, như một phương tiện để thúc đẩy sức khỏe tâm thần tốt hơn ở trẻ em và thanh thiếu niên."} +{"idx": 1870646, "en": "PURPOSE OF REVIEW The focus of this review is to discuss obesity, physical activity (and physical inactivity/sedentary behavior), cardiovascular disease (CVD), and their often interrelated health implications. The authors summarize the pathophysiological changes associated with obesity, which lead to the development of CVD, recommendations for interventions such as diet, increased physical activity, and weight loss according to current literature and guidelines, and the critical importance of cardiorespiratory fitness (CRF). RECENT FINDINGS Clinical trials continue to demonstrate improved outcomes among overweight or obese individuals who achieve a healthy weight using various methods. Increasing CRF levels appears to demonstrate the largest health improvements, regardless of underlying comorbidities or achieving weight loss. CRF, which is perhaps the single most important predictor of overall health, seems more important than weight loss alone regarding improved CVD outcomes in the obese population. These findings are reproduced in studies involving patients with various forms of CVD and CVD risk factors. The importance of CRF is well established; future endeavors to establish specific CRF targets for various patient cohorts are needed.", "gemini": "MỤC ĐÍCH CỦA BÀI ĐÁNH GIÁ Trọng tâm của bài đánh giá này là thảo luận về béo phì, hoạt động thể chất (và ít vận động/ngồi nhiều), bệnh tim mạch (CVD) và những tác động thường liên quan đến sức khỏe của chúng. Các tác giả tóm tắt các thay đổi bệnh sinh lý liên quan đến béo phì, dẫn đến sự phát triển của CVD, các khuyến nghị về các biện pháp can thiệp như chế độ ăn uống, tăng cường hoạt động thể chất và giảm cân theo các tài liệu và hướng dẫn hiện tại, và tầm quan trọng của thể lực tim mạch hô hấp (CRF). PHÁT HIỆN GẦN ĐÂY Các thử nghiệm lâm sàng tiếp tục chứng minh kết quả cải thiện ở những người thừa cân hoặc béo phì đạt được cân nặng khỏe mạnh bằng nhiều phương pháp khác nhau. Việc tăng mức CRF dường như chứng minh những cải thiện sức khỏe lớn nhất, bất kể các bệnh đi kèm tiềm ẩn hoặc đạt được mục tiêu giảm cân. CRF, có lẽ là yếu tố dự báo quan trọng nhất về sức khỏe tổng thể, có vẻ quan trọng hơn việc giảm cân đơn thuần đối với việc cải thiện kết quả CVD ở những người béo phì. Những phát hiện này được tái hiện trong các nghiên cứu liên quan đến những bệnh nhân mắc nhiều dạng CVD và các yếu tố nguy cơ CVD. Tầm quan trọng của CRF đã được thiết lập rõ ràng; cần có những nỗ lực trong tương lai để thiết lập các mục tiêu CRF cụ thể cho các nhóm bệnh nhân khác nhau."} +{"idx": 1870647, "en": "INTRODUCTION Postprandial hyperglycemia is independently associated with many adverse complications, while diets with a low glycemic load are beneficial in improving post-meal glucose levels. This study aims to determine if mobile-app-based low-carbohydrate dietary guidance will reduce exposure to postprandial hyperglycemia in adults with prediabetes. METHODS This single-blind, randomized controlled pilot study included 100 participants (39 men; mean age 53.6 ± 11.9 years) and was performed in the PKU Care CNOOC Hospital, China. The intervention group (n = 57) received low-carbohydrate dietary guidance through a moblie app (CAReNA) for 3 months, while the control group (n = 43) only received health education on a diabetic diet. The primary outcome was change in time of postprandial hyperglycemia between baseline and 3 months. RESULTS The study revealed that the mean time in postprandial hyperglycemia (> 7.8 mmol/l [140 mg/dl]) monitored by flash glucose monitoring changed from 3.27 h/day at baseline to 2.34 h/day at 3 months in the intervention group and from 3.08 h/day to 2.96 h/day in the control group, with a between-group difference of - 0.81 h/day (P < 0.05). Fasting plasma glucose and glycated hemoglobin (HbA1c) in the intervention group decreased significantly, although no significant difference was seen between the two groups. Compared with the control group, the intervention group had a significant decrease in anthropometric and body composition measurements as well as triglycerides. CONCLUSION The mobile-app-based low-carbohydrate dietary guidance effectively reduced the time spent in postprandial hyperglycemia in adults with prediabetes. This new type of nutritional management has beneficial effects on people with prediabetes and needs further research. CLINICAL TRIAL REGISTRATION ChiCTR1900024880.", "gemini": "GIỚI THIỆU Tăng đường huyết sau ăn liên quan độc lập với nhiều biến chứng bất lợi, trong khi chế độ ăn ít tải lượng đường huyết có lợi trong việc cải thiện lượng glucose sau bữa ăn. Nghiên cứu này nhằm xác định xem hướng dẫn chế độ ăn ít carbohydrate dựa trên ứng dụng di động có làm giảm tình trạng tăng đường huyết sau ăn ở người lớn tiền tiểu đường hay không. PHƯƠNG PHÁP Nghiên cứu thí điểm có đối chứng ngẫu nhiên, mù đơn này bao gồm 100 người tham gia (39 nam; độ tuổi trung bình 53,6 ± 11,9 tuổi) và được thực hiện tại Bệnh viện CNOOC PKU Care, Trung Quốc. Nhóm can thiệp (n = 57) nhận được hướng dẫn chế độ ăn ít carbohydrate thông qua ứng dụng di động (CAReNA) trong 3 tháng, trong khi nhóm đối chứng (n = 43) chỉ nhận được giáo dục sức khỏe về chế độ ăn cho bệnh nhân tiểu đường. Kết quả chính là sự thay đổi thời gian tăng đường huyết sau ăn giữa thời điểm ban đầu và 3 tháng. KẾT QUẢ Nghiên cứu cho thấy thời gian trung bình tăng đường huyết sau ăn (> 7,8 mmol/l [140 mg/dl]) được theo dõi bằng cách theo dõi glucose nhanh đã thay đổi từ 3,27 giờ/ngày khi bắt đầu thành 2,34 giờ/ngày sau 3 tháng ở nhóm can thiệp và từ 3,08 giờ/ngày thành 2,96 giờ/ngày ở nhóm đối chứng, với chênh lệch giữa các nhóm là - 0,81 giờ/ngày (P < 0,05). Glucose huyết tương lúc đói và hemoglobin glycat (HbA1c) ở nhóm can thiệp giảm đáng kể, mặc dù không thấy sự khác biệt đáng kể nào giữa hai nhóm. So với nhóm đối chứng, nhóm can thiệp có sự giảm đáng kể về các phép đo thành phần cơ thể và nhân trắc học cũng như triglycerid. KẾT LUẬN Hướng dẫn chế độ ăn ít carbohydrate dựa trên ứng dụng di động làm giảm hiệu quả thời gian tăng đường huyết sau ăn ở người lớn tiền tiểu đường. Loại quản lý dinh dưỡng mới này có tác dụng có lợi đối với những người tiền tiểu đường và cần được nghiên cứu thêm. ĐĂNG KÝ THỬ NGHIỆM LÂM SÀNG ChiCTR1900024880."} +{"idx": 1870648, "en": "Migraine is a common and debilitating headache disorder. Although its pathogenesis remains elusive, abnormal trigeminal and central nervous system activity is likely to play an important role. Transient receptor potential (TRP) channels, which transduce noxious stimuli into pain signals, are expressed in trigeminal ganglion neurons and brain regions closely associated with the pathophysiology of migraine. In the trigeminal ganglion, TRP channels co-localize with calcitonin gene-related peptide, a neuropeptide crucially implicated in migraine pathophysiology. Many preclinical and clinical data support the roles of TRP channels in migraine. In particular, activation of TRP cation channel V1 has been shown to regulate calcitonin gene-related peptide release from trigeminal nerves. Intriguingly, several effective anti-migraine therapies, including botulinum neurotoxin type A, affect the functions of TRP cation channels. Here, we discuss currently available data regarding the roles of major TRP cation channels in the pathophysiology of migraine and the therapeutic applicability thereof.", "gemini": "Đau nửa đầu là một rối loạn đau đầu phổ biến và gây suy nhược. Mặc dù cơ chế sinh bệnh của nó vẫn còn khó nắm bắt, nhưng hoạt động bất thường của hệ thần kinh trung ương và dây sinh ba có thể đóng vai trò quan trọng. Các kênh thụ thể thế năng thoáng qua (TRP), chuyển đổi các kích thích có hại thành tín hiệu đau, được biểu hiện ở các tế bào thần kinh hạch sinh ba và các vùng não liên quan chặt chẽ đến bệnh sinh lý của chứng đau nửa đầu. Trong hạch sinh ba, các kênh TRP đồng định vị với peptide liên quan đến gen calcitonin, một neuropeptit có liên quan mật thiết đến bệnh sinh lý của chứng đau nửa đầu. Nhiều dữ liệu tiền lâm sàng và lâm sàng hỗ trợ vai trò của các kênh TRP trong chứng đau nửa đầu. Đặc biệt, hoạt hóa kênh cation TRP V1 đã được chứng minh là điều chỉnh giải phóng peptide liên quan đến gen calcitonin từ các dây thần kinh sinh ba. Thật thú vị, một số liệu pháp chống đau nửa đầu hiệu quả, bao gồm cả độc tố thần kinh botulinum loại A, ảnh hưởng đến chức năng của các kênh cation TRP. Ở đây, chúng tôi thảo luận về dữ liệu hiện có về vai trò của các kênh cation TRP chính trong bệnh sinh lý của chứng đau nửa đầu và khả năng áp dụng điều trị của chúng."} +{"idx": 1870649, "en": "PURPOSE The length of time a critically ill coronavirus disease 2019 (COVID-19) patient remains infectious and should therefore be isolated remains unknown. This prospective study was undertaken in critically ill patients to evaluate the reliability of single negative real-time polymerase chain reaction (RT-PCR) in lower tracheal aspirates (LTA) in predicting a second negative test and to analyze clinical factors potentially influencing the viral shedding. METHODS From April 9, 2020 onwards, intubated COVID-19 patients treated in the intensive care unit were systematically evaluated for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by RT-PCR of nasopharyngeal swabs and LTA. The time to negativity was defined as the time between the onset of symptoms and the viral clearance in LTA. In order to identify risk factors for prolonged viral shedding, we used univariate and multivariate Cox proportional hazards models. RESULTS Forty-eight intubated SARS-CoV-2 patients were enrolled. Overall, we observed that the association of the first negative RT-PCR with a second negative result was 96.7%. Median viral shedding was 25 (IQR: 21.5-28) days since symptoms' onset. In the univariate Cox model analysis, type 2 diabetes mellitus was associated with a prolonged viral RNA shedding (hazard ratio [HR]: 0.41, 95% CI: 0.06-3.11, p = 0.04). In the multivariate Cox model analysis, type 2 diabetes was associated with a prolonged viral RNA shedding (HR: 0.31, 95% CI: 0.11-0.89, p = 0.029). CONCLUSION Intubated patients with type 2 diabetes mellitus may have prolonged SARS-CoV-2 shedding. In critically ill COVID-19 patients, one negative LTA should be sufficient to assess and exclude infectivity.", "gemini": "MỤC ĐÍCH Thời gian một bệnh nhân mắc bệnh do vi-rút corona 2019 (COVID-19) nguy kịch vẫn còn khả năng lây nhiễm và do đó nên được cách ly vẫn chưa được biết. Nghiên cứu triển vọng này được thực hiện ở những bệnh nhân nguy kịch để đánh giá độ tin cậy của phản ứng chuỗi polymerase thời gian thực âm tính đơn lẻ (RT-PCR) trong dịch hút khí quản dưới (LTA) trong việc dự đoán xét nghiệm âm tính thứ hai và phân tích các yếu tố lâm sàng có khả năng ảnh hưởng đến sự phát tán vi-rút. PHƯƠNG PHÁP Từ ngày 9 tháng 4 năm 2020 trở đi, những bệnh nhân COVID-19 được đặt nội khí quản điều trị tại khoa hồi sức tích cực được đánh giá có hệ thống về hội chứng hô hấp cấp tính nặng do vi-rút corona 2 (SARS-CoV-2) bằng RT-PCR của dịch tỵ hầu và LTA. Thời gian để âm tính được định nghĩa là thời gian giữa khi khởi phát các triệu chứng và khi vi-rút được thanh thải khỏi LTA. Để xác định các yếu tố nguy cơ gây phát tán vi-rút kéo dài, chúng tôi đã sử dụng các mô hình tỷ lệ nguy cơ tỷ lệ Cox đơn biến và đa biến. KẾT QUẢ Có 48 bệnh nhân SARS-CoV-2 được đặt nội khí quản được ghi danh. Nhìn chung, chúng tôi quan sát thấy rằng mối liên quan giữa RT-PCR âm tính đầu tiên với kết quả âm tính thứ hai là 96,7%. Thời gian phát tán vi-rút trung bình là 25 (IQR: 21,5-28) ngày kể từ khi khởi phát các triệu chứng. Trong phân tích mô hình Cox đơn biến, đái tháo đường tuýp 2 có liên quan đến việc phát tán RNA vi-rút kéo dài (tỷ lệ nguy cơ [HR]: 0,41, KTCB 95%: 0,06-3,11, p = 0,04). Trong phân tích mô hình Cox đa biến, đái tháo đường tuýp 2 có liên quan đến việc phát tán RNA vi-rút kéo dài (HR: 0,31, KTCB 95%: 0,11-0,89, p = 0,029). KẾT LUẬN Những bệnh nhân được đặt nội khí quản mắc bệnh đái tháo đường tuýp 2 có thể kéo dài thời gian phát tán SARS-CoV-2. Ở những bệnh nhân COVID-19 nguy kịch, một LTA âm tính là đủ để đánh giá và loại trừ khả năng lây nhiễm."} +{"idx": 1870650, "en": "PURPOSE To evaluate the effect of the positioning from the supine position (SP) to the prone position (PP) on the diaphragm activity in ventilated patients; using the ultrasound (US) imaging. METHODS A cross-sectional comparative study before/after PP was conducted on 40 ICU patients over 18 years who received invasive ventilation (IV) for at least 48 h. The considered ventilator modes were: assisted control volume with a low trigger flow (between - 2 and 2 L/mn) and pressure support mode. US diaphragmatic assessments were performed at SP and at 60 min of PP. Both End-inspiratory and End-expiratory diameters (EID/EED) were taken at 3 levels of axillary lines and determined by the average values of multiple measures. Diaphragmatic thickening fraction (DTF) was calculated as: DTF = (EID - EED/EED) × 100. Pairing and ANOVA tests were used for comparisons. RESULTS Forty ventilated patients (42 years of median age) at 4 days [2-7] of median duration of ventilation were examined during the two positions: SP versus PP. EID decreased from the SP to the PP (2.8 mm in SP vs. 2.4 mm in PP, p = 0.001). No difference was showed regarding the expiratory thickness. Overall, DTF didn't change in PP (37.4 vs. 42.05%, p = 0.36). When the patient was placed in PP, the best DTF value was showed at the posterior part of diaphragm (posterior: 45%, median: 31% and anterior: 38%, p = 0.049). CONCLUSION The ventral placement in ventilated patients reduced end-inspiratory diameter and tended to decrease DTF. In PP, the best contractile activity was detected at the posterior region of diaphragm.", "gemini": "MỤC ĐÍCH Đánh giá tác động của việc định vị từ tư thế nằm ngửa (SP) sang tư thế nằm sấp (PP) lên hoạt động của cơ hoành ở bệnh nhân thở máy; sử dụng hình ảnh siêu âm (US). PHƯƠNG PHÁP Một nghiên cứu so sánh cắt ngang trước / sau PP đã được tiến hành trên 40 bệnh nhân ICU trên 18 tuổi được thở máy xâm lấn (IV) trong ít nhất 48 giờ. Các chế độ thở máy được xem xét là: thể tích kiểm soát hỗ trợ với lưu lượng kích hoạt thấp (giữa -2 và 2 L / phút) và chế độ hỗ trợ áp lực. Đánh giá cơ hoành bằng siêu âm được thực hiện tại SP và tại 60 phút của PP. Đường kính cuối thì hít vào và cuối thì thở ra (EID / EED) được lấy ở 3 mức đường nách và được xác định bằng giá trị trung bình của nhiều phép đo. Độ dày cơ hoành (DTF) được tính như sau: DTF = (EID - EED / EED) x 100. Các phép thử ghép nối và ANOVA được sử dụng để so sánh. KẾT QUẢ Bốn mươi bệnh nhân thở máy (tuổi trung bình 42) ở thời điểm trung bình 4 ngày [2-7] thở máy đã được kiểm tra trong hai tư thế: SP so với PP. EID giảm từ SP xuống PP (2,8 mm ở SP so với 2,4 mm ở PP, p = 0,001). Không có sự khác biệt nào được thể hiện liên quan đến độ dày khi thở ra. Nhìn chung, DTF không thay đổi ở PP (37,4 so với 42,05%, p = 0,36). Khi bệnh nhân được đặt ở PP, giá trị DTF tốt nhất được thể hiện ở phần sau của cơ hoành (phía sau: 45%, trung bình: 31% và phía trước: 38%, p = 0,049). KẾT LUẬN Đặt ở tư thế nằm ngửa ở bệnh nhân thở máy làm giảm đường kính cuối thì hít vào và có xu hướng làm giảm DTF. Ở PP, hoạt động co bóp tốt nhất được phát hiện ở vùng sau của cơ hoành."} +{"idx": 1870651, "en": "Indicators of ecosystem health are effective tools to assess freshwater ecosystem impairment. However, they are scarcely used as a monitoring tool by local environmental agencies in Argentina. Here, we review the literature to analyze the use of ecosystem health indicators in freshwaters from Argentina. We found 91 scientific articles relating to the use of ecological indices to assess the impact of different environmental stressors in aquatic environments published between 1996 and 2019. We generated Google Earth map where we deployed the sampling sites and type of indices reported by each article. As biological indices were the most used, we also surveyed bioindication experts to gather information on their application. We found that most studies were concentrated mainly in Pampas (34%), Dry Chaco (20%), Espinal (12%), and Patagonian Steppe (10%) ecoregions. Biological indices (mainly with invertebrates) were more used than geomorphological or physico-chemical indices. Indices resulted useful to evaluate the impact of stressors in 63% of cases, being land use the most studied stressor. However, sampling design varied greatly among studies, making their comparison difficult. The information compiled here could help to the design of monitoring protocols, the adoption of regional indices, and the creation of a national inventory of ecosystem health status, which are mandatory to propose well-grounded conservation and management policies for freshwaters in Argentina.", "gemini": "Các chỉ số về sức khỏe hệ sinh thái là những công cụ hiệu quả để đánh giá tình trạng suy giảm hệ sinh thái nước ngọt. Tuy nhiên, chúng hiếm khi được các cơ quan môi trường địa phương ở Argentina sử dụng như một công cụ giám sát. Ở đây, chúng tôi xem xét các tài liệu để phân tích việc sử dụng các chỉ số sức khỏe hệ sinh thái trong nước ngọt từ Argentina. Chúng tôi đã tìm thấy 91 bài báo khoa học liên quan đến việc sử dụng các chỉ số sinh thái để đánh giá tác động của các tác nhân gây căng thẳng môi trường khác nhau trong môi trường nước được xuất bản từ năm 1996 đến năm 2019. Chúng tôi đã tạo bản đồ Google Earth nơi chúng tôi triển khai các địa điểm lấy mẫu và loại chỉ số được báo cáo bởi từng bài báo. Vì các chỉ số sinh học được sử dụng nhiều nhất, chúng tôi cũng đã khảo sát các chuyên gia về chỉ định sinh học để thu thập thông tin về ứng dụng của họ. Chúng tôi thấy rằng hầu hết các nghiên cứu tập trung chủ yếu ở các vùng sinh thái Pampas (34%), Dry Chaco (20%), Espinal (12%) và Patagonian Steppe (10%). Các chỉ số sinh học (chủ yếu là với các loài động vật không xương sống) được sử dụng nhiều hơn các chỉ số địa mạo hoặc vật lý hóa học. Các chỉ số này hữu ích để đánh giá tác động của các tác nhân gây căng thẳng trong 63% các trường hợp, trong đó sử dụng đất là tác nhân gây căng thẳng được nghiên cứu nhiều nhất. Tuy nhiên, thiết kế lấy mẫu thay đổi rất nhiều giữa các nghiên cứu, khiến việc so sánh chúng trở nên khó khăn. Thông tin được biên soạn ở đây có thể giúp thiết kế các giao thức giám sát, áp dụng các chỉ số khu vực và tạo ra một bản kiểm kê quốc gia về tình trạng sức khỏe hệ sinh thái, đây là điều bắt buộc để đề xuất các chính sách bảo tồn và quản lý có cơ sở vững chắc cho nguồn nước ngọt ở Argentina."} +{"idx": 1870652, "en": "Immunotherapy is currently approved for a subset of patients diagnosed with advanced triple negative breast cancer (TNBC), based on the phase III randomized controlled trial, IMpassion130. The anti-programmed cell death ligand-1 (PD-L1) immune checkpoint inhibitor atezolizumab combined with nanoparticle albumin-bound (nab)-paclitaxel is currently the standard first-line therapy in patients with metastatic TNBC who have a PD-L1-positive peritumoral immune infiltrate. Although this approval is limited to only a subset of patients, strategies to expand indications in breast cancer for this treatment modality are being extensively evaluated. A substantial need exists for the identification of patient characteristics, disease settings, immune markers, ideal partners for combination with immune checkpoint inhibitors, and the ideal sequence with traditional anticancer therapies. Additionally, in light of the results of the KEYNOTE-522 study of adjuvant pembrolizumab in TNBC, evaluation of immunotherapy in the early disease setting is a subject of great interest. This review article discusses current knowledge on immune checkpoint inhibitors in clinical practice, and provides an overview of a variety of markers evaluated to predict benefit of immunotherapy and of promising new strategies to enhance immune response and enable more patients to benefit from immunotherapy.", "gemini": "Miễn dịch trị liệu hiện được chấp thuận cho một nhóm bệnh nhân được chẩn đoán mắc ung thư vú ba âm tính tiến triển (TNBC), dựa trên thử nghiệm lâm sàng ngẫu nhiên có đối chứng pha III, IMpassion130. Thuốc ức chế điểm kiểm soát miễn dịch tế bào chết theo chương trình -1 (PD-L1) atezolizumab kết hợp với nab-paclitaxel liên kết với albumin dạng hạt nano (nab) hiện là liệu pháp tiêu chuẩn一线 ở những bệnh nhân mắc TNBC di căn có thâm nhiễm miễn dịch quanh khối u dương tính với PD-L1. Mặc dù sự chấp thuận này chỉ giới hạn ở một nhóm bệnh nhân, các chiến lược mở rộng chỉ định trong ung thư vú cho phương thức điều trị này đang được đánh giá rộng rãi. Có nhu cầu đáng kể trong việc xác định các đặc điểm của bệnh nhân, bối cảnh bệnh, các dấu ấn miễn dịch, các đối tác lý tưởng để kết hợp với thuốc ức chế điểm kiểm soát miễn dịch và trình tự lý tưởng với các liệu pháp chống ung thư truyền thống. Ngoài ra, theo kết quả của nghiên cứu KEYNOTE-522 về pembrolizumab bổ trợ ở TNBC, việc đánh giá miễn dịch trị liệu trong bối cảnh bệnh giai đoạn sớm là một chủ đề rất được quan tâm. Bài tổng quan này thảo luận về kiến thức hiện tại về thuốc ức chế điểm kiểm soát miễn dịch trong thực hành lâm sàng và cung cấp tổng quan về nhiều loại dấu ấn được đánh giá để dự đoán lợi ích của miễn dịch trị liệu và các chiến lược mới đầy hứa hẹn để tăng cường đáp ứng miễn dịch và giúp nhiều bệnh nhân hơn có thể hưởng lợi từ miễn dịch trị liệu."} +{"idx": 1870653, "en": "Identification of toxicants that underlie neurological diseases is a neglected area awaiting a valid strategy to identify such toxicants. We sought biomarkers that respond to known neurotoxicants in LUHMES immortalized neurons and evaluated these biomarkers for use in screening libraries of environmental toxicants. LUHMES immortalized human dopaminergic neurons were surveyed by RNA sequencing following challenge with parkinsonian toxicants rotenone, 6-hydroxydopamine, MPP+, and ziram (zinc dimethyldithiocarbamate; Zn2+DDC2), as well as additional toxicants paraquat, MS275, and methylmercury. The metallothionein gene MT1G was the most dynamic gene expression response to all seven toxicants. Multiple toxicants also increased transcripts for SLC30A1 and SLC30A2 zinc secretion transporters, the SLC7A11 xCT cystine/glutamate antiporter important for glutathione synthesis, DNA damage inducible transcript 3 (DDIT3), and secreted growth factors FIBIN and CXCL12, whereas several toxicants decreased expression of the apelin growth factor (APLN). These biomarker genes revealed stress responses to many toxicants at sub-cytotoxic concentrations. Since several of these biomarker genes and prior neurological disease studies implicated disruption of metal distribution, we tested metal chelator thiram (dimethyldithiocarbamate, DDC), ziram, and several other metals and metal chelates for cytotoxicity and induction of MT1G expression. Metals and chelators that caused dynamic increases in MT1G expression also caused cytotoxicity, except Ni2+DDC2 induced MT1G at 5 μM, but lacked cytotoxicity up to 100 μM. These results bolster prior work suggesting that neurons are characteristically sensitive to depletion of glutathione or to disruption of cellular metal distribution and provide biomarkers to search for such neurotoxicants in chemical libraries.", "gemini": "Việc xác định các chất độc gây ra các bệnh về thần kinh là một lĩnh vực bị bỏ quên đang chờ một chiến lược hợp lệ để xác định các chất độc như vậy. Chúng tôi đã tìm kiếm các dấu ấn sinh học phản ứng với các chất độc thần kinh đã biết trong các tế bào thần kinh bất tử LUHMES và đánh giá các dấu ấn sinh học này để sử dụng trong các thư viện sàng lọc các chất độc trong môi trường. Các tế bào thần kinh dopaminergic ở người bất tử LUHMES đã được khảo sát bằng giải trình tự RNA sau khi thử thách bằng các chất độc thần kinh parkinson rotenone, 6-hydroxydopamine, MPP + và ziram (kẽm dimethyldithiocarbamate; Zn2 + DDC2), cũng như các chất độc bổ sung paraquat, MS275 và methylmercury. Gen metallothionein MT1G là đáp ứng biểu hiện gen động nhất đối với cả bảy chất độc. Nhiều chất độc cũng làm tăng bản sao cho các chất vận chuyển tiết kẽm SLC30A1 và SLC30A2, chất vận chuyển cystine / glutamate SLC7A11 xCT quan trọng cho quá trình tổng hợp glutathione, bản sao có thể gây tổn thương DNA 3 (DDIT3) và các yếu tố tăng trưởng tiết ra FIBIN và CXCL12, trong khi một số chất độc làm giảm biểu hiện của yếu tố tăng trưởng apelin (APLN). Các gen dấu ấn sinh học này cho thấy phản ứng căng thẳng với nhiều chất độc ở nồng độ dưới độc tế bào. Vì một số gen dấu ấn sinh học này và các nghiên cứu trước đây về bệnh thần kinh liên quan đến sự gián đoạn phân phối kim loại, chúng tôi đã thử nghiệm chất tạo phức kim loại thiram (dimethyldithiocarbamate, DDC), ziram và một số kim loại khác và chelat kim loại để tìm độc tính tế bào và cảm ứng biểu hiện MT1G. Các kim loại và chất tạo phức gây ra sự gia tăng động trong biểu hiện MT1G cũng gây ra độc tính tế bào, ngoại trừ Ni2 + DDC2 gây ra MT1G ở 5 μM, nhưng không gây độc tế bào lên đến 100 μM. Những kết quả này củng cố công trình trước đây cho thấy các tế bào thần kinh đặc biệt nhạy cảm với sự suy giảm glutathione hoặc sự gián đoạn phân phối kim loại trong tế bào và cung cấp các dấu ấn sinh học để tìm kiếm các chất độc thần kinh như vậy trong các thư viện hóa học."} +{"idx": 1870654, "en": "PURPOSE This study aimed to establish which determinants had an effect on frailty among acutely admitted patients, where frailty was identified at discharge. In particular, our study focused on associations of sex with frailty. METHODS A cross-sectional study was designed using a sample of 1267 people aged 65 years or older. The Tilburg Frailty Indicator (TFI), a user-friendly self-report questionnaire was used to measure multidimensional frailty (physical, psychological, social) and determinants of frailty (sex, age, marital status, education, income, lifestyle, life events, multimorbidity). RESULTS The mean age of the participants was 76.8 years (SD 7.5; range 65-100). The bivariate regression analyses showed that all determinants were associated with total and physical frailty, and six determinants were associated with psychological and social frailty. Using multiple linear regression analyses, the explained variances differed from 3.5% (psychological frailty) to 20.1% (social frailty), with p values < 0.001. Of the independent variables age, income, lifestyle, life events, and multimorbidity were associated with three frailty variables, after controlling for all the other variables in the model. At the level of both frailty domains and components, females appeared to be more frail than men. CONCLUSION The present study showed that sociodemographic characteristics (sex, age, marital status, education, income), lifestyle, life events, and multimorbidity had a different effect on total frailty and its domains (physical, psychological, social) in a sample of acute admitted patients.", "gemini": "MỤC ĐÍCH Nghiên cứu này nhằm xác định những yếu tố quyết định nào có ảnh hưởng đến tình trạng suy yếu ở những bệnh nhân nhập viện cấp cứu, khi tình trạng suy yếu được xác định khi xuất viện. Đặc biệt, nghiên cứu của chúng tôi tập trung vào mối liên quan của giới tính với tình trạng suy yếu. PHƯƠNG PHÁP Một nghiên cứu cắt ngang đã được thiết kế sử dụng mẫu gồm 1267 người từ 65 tuổi trở lên. Chỉ số suy yếu Tilburg (TFI), một bảng câu hỏi tự báo cáo thân thiện với người dùng đã được sử dụng để đo lường tình trạng suy yếu đa chiều (thể chất, tâm lý, xã hội) và các yếu tố quyết định tình trạng suy yếu (giới tính, tuổi, tình trạng hôn nhân, trình độ học vấn, thu nhập, lối sống, các sự kiện cuộc sống, đa bệnh). KẾT QUẢ Độ tuổi trung bình của những người tham gia là 76,8 tuổi (SD 7,5; phạm vi 65-100). Các phân tích hồi quy hai biến cho thấy tất cả các yếu tố quyết định đều có liên quan đến tình trạng suy yếu về thể chất và tổng thể, và sáu yếu tố quyết định có liên quan đến tình trạng suy yếu về tâm lý và xã hội. Sử dụng các phân tích hồi quy tuyến tính đa biến, các phương sai đã giải thích khác nhau từ 3,5% (suy yếu về tâm lý) đến 20,1% (suy yếu về xã hội), với giá trị p < 0,001. Trong số các biến độc lập là tuổi, thu nhập, lối sống, các sự kiện cuộc sống và đa bệnh có liên quan đến ba biến suy yếu, sau khi kiểm soát tất cả các biến khác trong mô hình. Ở cả mức độ thành phần và phạm vi suy yếu, phụ nữ dường như yếu hơn nam giới. KẾT LUẬN Nghiên cứu hiện tại cho thấy các đặc điểm nhân khẩu học xã hội (giới tính, tuổi, tình trạng hôn nhân, trình độ học vấn, thu nhập), lối sống, các sự kiện cuộc sống và đa bệnh có ảnh hưởng khác nhau đến tình trạng suy yếu tổng thể và các phạm vi của nó (thể chất, tâm lý, xã hội) trong một mẫu bệnh nhân nhập viện cấp cứu."} +{"idx": 1870655, "en": "PURPOSE It is important to identify which older patients attending the emergency department are at risk of adverse outcomes to introduce preventive interventions. This study aimed to assess the prognostic value of a shortened screening instrument based on the Dutch national Safety Management System [Veiligheidsmanagementsysteem (VMS)] guidelines for adverse outcomes in older emergency department patients. METHODS A cohort study was performed including patients aged 70 years or older who visited the emergency department. Adverse outcomes included hospital admission, return emergency department visits within 30 days, and 90-day mortality. The prognostic value of the VMS-score was assessed for these adverse events and, in addition, a prediction model was developed for 90-day mortality. RESULTS A high VMS-score was independently associated with an increased risk of hospital admission [OR 2.26 (95% CI 1.32-3.86)] and 90-day mortality [HR 2.48 (95% CI 1.31-4.71)]. The individual VMS-questions regarding history of delirium and help in activities of daily living were associated with these outcomes as well. A prediction model for 90-day mortality was developed and showed satisfactory calibration and good discrimination [AUC 0.80 (95% CI 0.72-0.87)]. A cut-off point that selected 30% of patients at the highest risk yielded a sensitivity of 67.4%, a specificity of 75.3%, a positive predictive value of 28.5%, and a negative predictive value of 94.1%. CONCLUSION The shortened VMS-based screening instrument showed to be of good prognostic value for hospitalization and 90-day mortality. The prediction model for mortality showed promising results and will be further validated and optimized.", "gemini": "MỤC ĐÍCH Điều quan trọng là phải xác định những bệnh nhân lớn tuổi nào đến khoa cấp cứu có nguy cơ gặp các kết cục bất lợi để đưa ra các biện pháp can thiệp phòng ngừa. Nghiên cứu này nhằm đánh giá giá trị tiên lượng của một công cụ sàng lọc rút gọn dựa trên các hướng dẫn của Hệ thống quản lý an toàn quốc gia Hà Lan [Veiligheidsmanagementsysteem (VMS)] đối với các kết cục bất lợi ở bệnh nhân cấp cứu lớn tuổi. PHƯƠNG PHÁP Một nghiên cứu theo dõi đã được thực hiện bao gồm những bệnh nhân từ 70 tuổi trở lên đến khoa cấp cứu. Các kết cục bất lợi bao gồm nhập viện, tái khám cấp cứu trong vòng 30 ngày và tử vong trong vòng 90 ngày. Giá trị tiên lượng của điểm số VMS đã được đánh giá đối với các biến cố bất lợi này và ngoài ra, một mô hình dự đoán đã được phát triển đối với tử vong trong vòng 90 ngày. KẾT QUẢ Điểm số VMS cao có liên quan độc lập với nguy cơ nhập viện tăng [OR 2,26 (95% CI 1,32-3,86)] và tử vong trong vòng 90 ngày [HR 2,48 (95% CI 1,31-4,71)]. Các câu hỏi riêng lẻ của VMS liên quan đến tiền sử lú lẫn và hỗ trợ trong các hoạt động sinh hoạt hàng ngày cũng có liên quan đến các kết cục này. Một mô hình dự đoán tử vong trong vòng 90 ngày đã được phát triển và cho thấy hiệu chuẩn thỏa đáng và phân biệt tốt [AUC 0,80 (95% CI 0,72-0,87)]. Điểm cắt chọn 30% bệnh nhân có nguy cơ cao nhất cho thấy độ nhạy là 67,4%, độ đặc hiệu là 75,3%, giá trị dự đoán dương tính là 28,5% và giá trị dự đoán âm tính là 94,1%. KẾT LUẬN Công cụ sàng lọc dựa trên VMS rút gọn cho thấy có giá trị tiên lượng tốt đối với tình trạng nhập viện và tử vong trong vòng 90 ngày. Mô hình dự đoán tử vong cho thấy kết quả hứa hẹn và sẽ được xác nhận và tối ưu hóa thêm."} +{"idx": 1870656, "en": "Chronic neurocognitive impairments, commonly associated with pediatric human immunodeficiency virus type 1 (PHIV), are a detrimental consequence of early exposure to HIV-1 viral proteins. Strong evidence supports S-Equol (SE) as an efficacious adjunctive neuroprotective and/or neurorestorative therapeutic for neurocognitive impairments in adult ovariectomized female HIV-1 transgenic (Tg) rats. There remains, however, a critical need to assess the therapeutic efficacy of SE when treatment occurs at an earlier age (i.e., resembling a therapeutic for children with PHIV) and across the factor of biological sex. Utilization of a series of signal detection operant tasks revealed prominent, sex-dependent neurocognitive deficits in the HIV-1 Tg rat, characterized by alterations in stimulus-reinforcement learning, the response profile, and temporal processing. Early (i.e., postnatal day 28) initiation of SE treatment precluded the development of chronic neurocognitive impairments in all (i.e., 100%) HIV-1 Tg animals, albeit not for all neurocognitive domains. Most notably, the therapeutic effects of SE are generalized across the factor of biological sex, despite the presence of endogenous hormones. Results support, therefore, the efficacy of SE as a neuroprotective therapeutic for chronic neurocognitive impairments in the post-cART era; an adjunctive therapeutic that demonstrates high efficacy in both males and females. Optimizing treatment conditions by evaluating multiple factors (i.e., age, neurocognitive domains, and biological sex) associated with PHIV and HIV-1 associated neurocognitive disorders (HAND) affords a key opportunity to improve the therapeutic efficacy of SE.", "gemini": "Những khiếm khuyết nhận thức thần kinh mạn tính, thường liên quan đến virus gây suy giảm miễn dịch ở người loại 1 ở trẻ em (PHIV), là hậu quả bất lợi của việc tiếp xúc sớm với các protein virus HIV-1. Có bằng chứng mạnh mẽ chứng minh S-Equol (SE) là một liệu pháp điều trị hiệu quả bổ sung bảo vệ thần kinh và/hoặc phục hồi thần kinh cho những khiếm khuyết nhận thức thần kinh ở những con chuột chuyển gen HIV-1 (Tg) cái bị cắt bỏ buồng trứng ở tuổi trưởng thành. Tuy nhiên, vẫn còn một nhu cầu quan trọng là phải đánh giá hiệu quả điều trị của SE khi điều trị ở độ tuổi sớm hơn (tức là giống như một liệu pháp điều trị cho trẻ em mắc PHIV) và trên yếu tố giới tính sinh học. Việc sử dụng một loạt các nhiệm vụ vận hành phát hiện tín hiệu cho thấy những khiếm khuyết nhận thức thần kinh phụ thuộc vào giới tính rõ rệt ở chuột HIV-1 Tg, đặc trưng bởi những thay đổi trong quá trình học củng cố kích thích, hồ sơ phản ứng và xử lý thời gian. Việc bắt đầu điều trị SE sớm (tức là ngày thứ 28 sau sinh) đã ngăn ngừa sự phát triển của những khiếm khuyết nhận thức thần kinh mạn tính ở tất cả (tức là 100%) động vật HIV-1 Tg, mặc dù không phải đối với tất cả các phạm vi nhận thức thần kinh. Đáng chú ý nhất là các tác dụng điều trị của SE được tổng quát trên yếu tố giới tính sinh học, mặc dù có sự hiện diện của hormone nội sinh. Do đó, kết quả nghiên cứu ủng hộ hiệu quả của SE như một liệu pháp điều trị bảo vệ thần kinh cho những khiếm khuyết nhận thức thần kinh mạn tính trong thời kỳ hậu cART; một liệu pháp điều trị bổ sung có hiệu quả cao ở cả nam và nữ. Việc tối ưu hóa các điều kiện điều trị bằng cách đánh giá nhiều yếu tố (tức là tuổi, phạm vi nhận thức thần kinh và giới tính sinh học) liên quan đến PHIV và các rối loạn nhận thức thần kinh liên quan đến HIV-1 (HAND) mang lại cơ hội chính để cải thiện hiệu quả điều trị của SE."} +{"idx": 1870657, "en": "Endoplasmic reticulum stress (ER stress) can be induced by virus infection. In this part, we explored whether Hantaan virus (HTNV) infection could induce ER stress in differentiated THP-1 (dTHP-1) cells. It showed that the mRNA and protein levels of ER stress-related 78 kDa glucose-regulated protein (GRP78, HSPA5) and mRNA levels of X box-binding protein 1 (XBP-1), activating transcription factor 6(ATF6) and PKR-like ER kinase (PERK) after HTNV infection, were significantly higher than that in uninfected control group. However, the mRNA levels of C/EBP homologous protein (CHOP), glucose-regulated protein 94 (GRP94, HSPC4), and inositol-requiring enzyme1 (IRE1) were not significantly different between the infected group and the untreated group in 2 h after virus infection. It is unusual in activating GRP78 but not GRP94. Meanwhile, dTHP-1 cells infected with HTNV at 12 h did not show obvious apoptosis. These results indicated that the HTNV infection could induce the unfolded protein response (UPR) in dTHP-1 cells, without directly leading to cell apoptosis during 12 h after virus infection.", "gemini": "Stress nội lưới nội bào (stress ER) có thể được gây ra bởi nhiễm virus. Trong phần này, chúng tôi đã khám phá xem nhiễm virus Hantaan (HTNV) có thể gây ra stress ER trong các tế bào THP-1 biệt hóa (dTHP-1) hay không. Kết quả cho thấy mức mRNA và protein của protein điều hòa glucose 78 kDa liên quan đến stress ER (GRP78, HSPA5) và mức mRNA của protein liên kết hộp X 1 (XBP-1), yếu tố phiên mã hoạt hóa 6 (ATF6) và kinase ER giống PKR (PERK) sau khi nhiễm HTNV, cao hơn đáng kể so với nhóm đối chứng không nhiễm. Tuy nhiên, mức mRNA của protein đồng đẳng C/EBP (CHOP), protein điều hòa glucose 94 (GRP94, HSPC4) và enzyme1 cần inositol (IRE1) không có sự khác biệt đáng kể giữa nhóm nhiễm và nhóm không được điều trị trong 2 giờ sau khi nhiễm virus. Việc kích hoạt GRP78 nhưng không phải GRP94 là điều bất thường. Trong khi đó, các tế bào dTHP-1 bị nhiễm HTNV ở giờ thứ 12 không biểu hiện rõ ràng quá trình apoptosis. Những kết quả này chỉ ra rằng nhiễm HTNV có thể gây ra phản ứng protein chưa gấp (UPR) trong các tế bào dTHP-1, mà không trực tiếp dẫn đến quá trình apoptosis của tế bào trong vòng 12 giờ sau khi nhiễm virus."} +{"idx": 1870658, "en": "Ischaemic stroke is an acute interruption of the blood supply to the brain, which leads to rapid irreversible damage to nerve tissue. Ischaemic stroke is accompanied by the development of neuroinflammation and neurodegeneration observed around the affected brain area. Heat shock protein 70 (Hsp70) facilitates cell survival under a variety of different stress conditions. Hsp70 may be secreted from cells and exhibits cytoprotective activity. This activity most likely occurs by decreasing the levels of several proinflammatory cytokines through interaction with a few receptors specific to the innate immune system. Herein, we demonstrated that intranasal administration of recombinant human Hsp70 shows a significant twofold decrease in the volume of local ischaemia induced by photothrombosis in the mouse prefrontal brain cortex. Our results revealed that intranasal injections of recombinant Hsp70 decreased the apoptosis level in the ischaemic penumbra, stimulated axonogenesis and increased the number of neurons producing synaptophysin. Similarly, in the isolated crayfish stretch receptor, consisting of a single sensory neuron surrounded by the glial envelope, exogenous Hsp70 significantly decreased photoinduced apoptosis and necrosis of glial cells. The obtained data enable one to consider human recombinant Hsp70 as a promising compound that could be translated from the bench into clinical therapies.", "gemini": "Đột quỵ thiếu máu cục bộ là sự gián đoạn cấp tính nguồn cung cấp máu lên não, dẫn đến tổn thương nhanh chóng không hồi phục đối với mô thần kinh. Đột quỵ thiếu máu cục bộ đi kèm với sự phát triển của tình trạng viêm thần kinh và thoái hóa thần kinh được quan sát thấy xung quanh vùng não bị ảnh hưởng. Protein sốc nhiệt 70 (Hsp70) thúc đẩy sự sống còn của tế bào trong nhiều điều kiện căng thẳng khác nhau. Hsp70 có thể được tiết ra từ các tế bào và biểu hiện hoạt động bảo vệ tế bào. Hoạt động này rất có thể xảy ra bằng cách làm giảm nồng độ của một số cytokine tiền viêm thông qua tương tác với một vài thụ thể đặc hiệu với hệ thống miễn dịch bẩm sinh. Trong bài báo này, chúng tôi chứng minh rằng việc sử dụng đường mũi của Hsp70 tái tổ hợp của người cho thấy sự giảm đáng kể gấp đôi thể tích cục bộ thiếu máu cục bộ do quang huyết khối ở vỏ não trước trán của chuột. Kết quả của chúng tôi cho thấy tiêm đường mũi Hsp70 tái tổ hợp làm giảm mức độ apoptosis ở vùng thiếu máu cục bộ, kích thích quá trình tạo trục và tăng số lượng tế bào thần kinh sản xuất synaptophysin. Tương tự, ở thụ thể kéo dài tôm càng xanh bị cô lập, bao gồm một tế bào thần kinh cảm giác duy nhất được bao quanh bởi lớp vỏ thần kinh đệm, Hsp70 ngoại sinh làm giảm đáng kể quá trình apoptosis do ánh sáng và hoại tử tế bào thần kinh đệm. Dữ liệu thu được cho phép coi Hsp70 tái tổ hợp của người là một hợp chất đầy hứa hẹn có thể được chuyển từ nghiên cứu cơ bản sang liệu pháp lâm sàng."} +{"idx": 1870659, "en": "An 82-year-old Japanese man with alcoholic liver cirrhosis was referred to our hospital for treatment of advanced esophageal cancer. A protruding tumor was endoscopically observed in the middle thoracic esophagus, and pathological findings of the biopsy specimens revealed a squamous cell carcinoma. The clinical tumor staging was stage II (T3N0M0). The patient received two courses of neoadjuvant chemotherapy with 5-fluorouracil and nedaplatin. After the treatments, computed tomography showed significant reductions in the size of the target tumor. However, radical esophagectomy was not performed because the patient refused major invasive treatments. Instead, endoscopic resection was performed using a combination of polypectomy and endoscopic submucosal resection (ESD). To prevent bleeding during endoscopic treatment, we applied a detachable snare to the base of the tumor and cut the stalk using by an SB knife Jr, without hemorrhage. The pathohistology of the resected specimen was positively showed cancer cells on the margin of the esophageal carcinoma stalk. At 4 weeks after the initial operation, an additional ESD was successfully performed, which pathologically led to radical removal. The patient survived for more than 18 months after beginning the initial treatment. We describe a successful treatment using endoscopic resection after chemotherapy for advanced esophageal cancer with high surgical treatment risks.", "gemini": "Một người đàn ông Nhật Bản 82 tuổi bị xơ gan do rượu đã được chuyển đến bệnh viện của chúng tôi để điều trị ung thư thực quản tiến triển. Một khối u lồi ra đã được quan sát bằng nội soi ở thực quản ngực giữa và các phát hiện bệnh lý của mẫu sinh thiết cho thấy ung thư biểu mô tế bào vảy. Giai đoạn khối u lâm sàng là giai đoạn II (T3N0M0). Bệnh nhân đã trải qua hai đợt hóa trị tân bổ trợ với 5-fluorouracil và nedaplatin. Sau các đợt điều trị, chụp cắt lớp vi tính cho thấy kích thước khối u mục tiêu giảm đáng kể. Tuy nhiên, phẫu thuật cắt bỏ thực quản triệt căn không được thực hiện vì bệnh nhân từ chối các phương pháp điều trị xâm lấn lớn. Thay vào đó, cắt bỏ nội soi được thực hiện bằng cách kết hợp cắt polyp và cắt bỏ nội soi dưới niêm mạc (ESD). Để ngăn ngừa chảy máu trong quá trình điều trị nội soi, chúng tôi đã áp dụng một vòng thắt có thể tháo rời vào gốc khối u và cắt cuống bằng dao SB Jr, không chảy máu. Giải phẫu bệnh của mẫu vật cắt bỏ cho thấy các tế bào ung thư ở rìa cuống ung thư thực quản. Sau 4 tuần kể từ ca phẫu thuật ban đầu, một ca ESD bổ sung đã được thực hiện thành công, về mặt bệnh học đã dẫn đến cắt bỏ triệt căn. Bệnh nhân sống sót hơn 18 tháng sau khi bắt đầu điều trị ban đầu. Chúng tôi mô tả một phương pháp điều trị thành công bằng cắt bỏ nội soi sau hóa trị đối với ung thư thực quản tiến triển có nguy cơ điều trị phẫu thuật cao."} +{"idx": 1870660, "en": "The antiviral agent remdesivir (Veklury®; Gilead Sciences), nucleotide analogue prodrug, has broad-spectrum activity against viruses from several families. Having demonstrated potent antiviral activity against coronaviruses in preclinical studies, remdesivir emerged as a candidate drug for the treatment of the novel coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, during the current global pandemic. Phase III evaluation of remdesivir in the treatment of COVID-19 commenced in early 2020 and has thus far yielded promising results. In late May 2020, Taiwan conditionally approved the use of remdesivir in patients with severe COVID-19. This was followed by a rapid succession of conditional approvals in various countries/regions including the EU and Canada. Preceding these conditional approvals, an emergency use authorization for remdesivir had been granted in the USA (on 1 May 2020) and a special approval for emergency use was granted in Japan (on 7 May 2020). This article summarizes the milestones in the development of remdesivir leading to its first conditional approval for the treatment of COVID-19.", "gemini": "Thuốc kháng vi-rút remdesivir (Veklury®; Gilead Sciences), tiền chất tương tự nucleotid, có hoạt tính phổ rộng chống lại vi-rút từ nhiều họ. Sau khi chứng minh được hoạt tính kháng vi-rút mạnh chống lại vi-rút corona trong các nghiên cứu tiền lâm sàng, remdesivir đã nổi lên như một loại thuốc ứng cử cho việc điều trị bệnh do vi-rút corona mới 2019 (COVID-19), do nhiễm vi-rút corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2), trong đại dịch toàn cầu hiện nay. Đánh giá giai đoạn III của remdesivir trong điều trị COVID-19 bắt đầu vào đầu năm 2020 và cho đến nay đã mang lại kết quả đầy hứa hẹn. Vào cuối tháng 5 năm 2020, Đài Loan đã phê duyệt có điều kiện việc sử dụng remdesivir ở những bệnh nhân mắc COVID-19 nặng. Tiếp theo là việc phê duyệt có điều kiện nhanh chóng ở nhiều quốc gia/khu vực khác nhau bao gồm EU và Canada. Trước những đợt phê duyệt có điều kiện này, remdesivir đã được cấp phép sử dụng khẩn cấp tại Hoa Kỳ (vào ngày 1 tháng 5 năm 2020) và được cấp phép đặc biệt để sử dụng khẩn cấp tại Nhật Bản (vào ngày 7 tháng 5 năm 2020). Bài viết này tóm tắt các mốc quan trọng trong quá trình phát triển remdesivir dẫn đến việc phê duyệt có điều kiện đầu tiên để điều trị COVID-19."} +{"idx": 1870661, "en": "In spore forming microbes, germination protease (GPR) plays a key role in the initiation of the germination process. A critical step during germination is the degradation of small acid-soluble proteins (SASPs), which protect spore DNA from external stresses (UV, heat, low temperature, etc.). Inactive zymogen GPR can be activated by autoprocessing of the N-terminal pro-sequence domain. Activated GPR initiates the degradation of SASPs; however, the detailed mechanisms underlying the activation, catalysis, regulation, and substrate recognition of GPR remain elusive. In this study, we determined the crystal structure of GPR from Paenisporosarcina sp. TG-20 (PaGPR) in its inactive form at a resolution of 2.5 A. Structural analysis showed that the active site of PaGPR is sterically occluded by an inhibitory loop region (residues 202-216). The N-terminal region interacts directly with the self-inhibitory loop region, suggesting that the removal of the N-terminal pro-sequence induces conformational changes, which lead to the release of the self-inhibitory loop region from the active site. In addition, comparative sequence and structural analyses revealed that PaGPR contains two highly conserved Asp residues (D123 and D182) in the active site, similar to the putative aspartic acid protease GPR from Bacillus megaterium. The catalytic domain structure of PaGPR also shares similarities with the sequentially non-homologous proteins HycI and HybD. HycI and HybD are metal-loproteases that also contain two Asp (or Glu) residues in their active site, playing a role in metal binding. In summary, our results provide useful insights into the activation process of PaGPR and its active conformation.", "gemini": "Trong các vi khuẩn hình thành bào tử, protease nảy mầm (GPR) đóng vai trò quan trọng trong việc khởi động quá trình nảy mầm. Một bước quan trọng trong quá trình nảy mầm là sự phân hủy các protein nhỏ hòa tan trong axit (SASP), có tác dụng bảo vệ DNA của bào tử khỏi các tác nhân gây căng thẳng bên ngoài (tia UV, nhiệt, nhiệt độ thấp, v.v.). GPR zymogen không hoạt động có thể được hoạt hóa bằng cách tự xử lý miền trình tự pro ở đầu N. GPR hoạt hóa khởi động quá trình phân hủy SASP; tuy nhiên, các cơ chế chi tiết cơ bản cho quá trình hoạt hóa, xúc tác, điều hòa và nhận biết cơ chất của GPR vẫn còn khó hiểu. Trong nghiên cứu này, chúng tôi đã xác định cấu trúc tinh thể của GPR từ Paenisporosarcina sp. TG-20 (PaGPR) ở dạng không hoạt động với độ phân giải 2,5 A. Phân tích cấu trúc cho thấy vị trí hoạt động của PaGPR bị che lấp về mặt không gian bởi một vùng vòng ức chế (phần còn lại 202-216). Vùng đầu N tương tác trực tiếp với vùng vòng tự ức chế, cho thấy rằng việc loại bỏ trình tự pro đầu N gây ra những thay đổi về cấu hình, dẫn đến việc giải phóng vùng vòng tự ức chế khỏi vị trí hoạt động. Ngoài ra, phân tích trình tự và cấu trúc so sánh cho thấy PaGPR chứa hai phần còn lại của Asp được bảo tồn cao (D123 và D182) ở vị trí hoạt động, tương tự như protease aspartic được cho là GPR từ Bacillus megaterium. Cấu trúc miền xúc tác của PaGPR cũng có những điểm tương đồng với các protein không đồng dạng tuần tự HycI và HybD. HycI và HybD là các metalloprotease cũng chứa hai phần còn lại của Asp (hoặc Glu) ở vị trí hoạt động, đóng vai trò liên kết kim loại. Tóm lại, kết quả của chúng tôi cung cấp những hiểu biết hữu ích về quá trình hoạt hóa của PaGPR và cấu hình hoạt động của nó."} +{"idx": 1870662, "en": "Brucella, the bacterial agent of common zoonotic brucellosis, primarily infects specific animal species. The Brucella outer membrane proteins (Omps) are particularly attractive for developing vaccine and improving diagnostic tests and are associated with the virulence of smooth Brucella strains. Omp16 is a homologue to peptidoglycan-associated lipoproteins (Pals), and an omp16 mutant has not been generated in any Brucella strain until now. Very little is known about the functions and pathogenic mechanisms of Omp16 in Brucella. Here, we confirmed that Omp16 has a conserved Pal domain and is highly conserved in Brucella. We attempted to delete omp16 in Brucella suis vaccine strain 2 (B. suis S2) without success, which shows that Omp16 is vital for Brucella survival. We acquired a B. suis S2 Omp16 mutant via conditional complementation. Omp16 deficiency impaired Brucella outer membrane integrity and activity in vitro. Moreover, inactivation of Omp16 decreased bacterial intracellular survival in macrophage RAW 264.7 cells. B. suis S2 and its derivatives induced marked expression of IL-1β, IL-6, and TNF-a mRNA in Raw 264.7 cells. Whereas inactivation of Omp16 in Brucella enhanced IL-1β and IL-6 expression in Raw 264.7 cells. Altogether, these findings show that the Brucella Omp16 mutant was obtained via conditional complementation and confirmed that Omp16 can maintain outer membrane integrity and be involved in bacterial virulence in Brucella in vitro and in vivo. These results will be important in uncovering the pathogenic mechanisms of Brucella.", "gemini": "Brucella, tác nhân gây bệnh brucella ở động vật phổ biến, chủ yếu lây nhiễm cho các loài động vật cụ thể. Các protein màng ngoài của Brucella (Omp) đặc biệt hấp dẫn để phát triển vắc-xin và cải thiện các xét nghiệm chẩn đoán và có liên quan đến độc lực của các chủng Brucella mịn. Omp16 là một đồng đẳng với các lipoprotein liên kết với peptidoglycan (Pals) và cho đến nay vẫn chưa tạo ra được đột biến omp16 ở bất kỳ chủng Brucella nào. Người ta biết rất ít về các chức năng và cơ chế gây bệnh của Omp16 trong Brucella. Ở đây, chúng tôi xác nhận rằng Omp16 có một miền Pal được bảo tồn và được bảo tồn cao trong Brucella. Chúng tôi đã cố gắng xóa omp16 ở chủng vắc-xin Brucella suis 2 (B. suis S2) nhưng không thành công, điều này cho thấy Omp16 rất cần thiết cho sự sống còn của Brucella. Chúng tôi đã thu được một đột biến B. suis S2 Omp16 thông qua bổ sung có điều kiện. Sự thiếu hụt Omp16 làm suy yếu tính toàn vẹn và hoạt động của màng ngoài Brucella trong ống nghiệm. Hơn nữa, việc bất hoạt Omp16 làm giảm sự sống còn của vi khuẩn trong tế bào đại thực RAW 264.7. B. suis S2 và các dẫn xuất của nó gây ra sự biểu hiện rõ rệt của mRNA IL-1β, IL-6 và TNF-a trong các tế bào Raw 264.7. Trong khi đó, việc bất hoạt Omp16 trong Brucella làm tăng biểu hiện IL-1β và IL-6 trong các tế bào Raw 264.7. Nhìn chung, những phát hiện này cho thấy rằng đột biến Brucella Omp16 đã thu được thông qua bổ sung có điều kiện và xác nhận rằng Omp16 có thể duy trì tính toàn vẹn của màng ngoài và tham gia vào độc lực của vi khuẩn ở Brucella trong ống nghiệm và trong cơ thể sống. Những kết quả này sẽ rất quan trọng trong việc khám phá các cơ chế gây bệnh của Brucella."} +{"idx": 1870663, "en": "Cryptococcus neoformans is an opportunistic fungal pathogen causing cryptococcal meningoencephalitis. Interestingly, the cell wall of C. neoformans contains chitosan, which is critical for its virulence and persistence in the mammalian host. C. neoformans (H99) has three chitin deacetylases (CDAs), which convert chitin to chitosan. Herein, the classification of the chitin-related protein (CRP) family focused on cryptococcal CDAs was analyzed by phylogenetics, evolutionary pressure (dN/dS), and 3D modeling. A phylogenetic tree of 110 CRPs revealed that they can be divided into two clades, CRP I and II with bootstrap values (> 99%). CRP I clade comprises five groups (Groups 1-5) with a total of 20 genes, while CRP II clade comprises sixteen groups (Groups 6-21) with a total of 90 genes. CRP I comprises only fungal CDAs, including all three C. neoformans CDAs, whereas CRP II comprises diverse CDAs from fungi, bacteria, and amoeba, along with other carbohydrate esterase 4 family proteins. All CDAs have the signal peptide, except those from group 11. Notably, CDAs with the putative O-gycosylation site possess either the glycosylphosphatidylinositol (GPI)-anchor motif for CRP I or the chitin-binding domain (CBD) for CRP II, respectively. This evolutionary conservation strongly indicates that the O-glycosylation modification and the presence of either the GPI-anchor motif or the chitin-binding domain is important for fungal CDAs to function efficiently at the cell surface. This study reveals that C. neoformans CDAs carrying GPI anchors have evolved divergently from fungal and bacterial CDAs, providing new insights into evolution and classification of CRP family.", "gemini": "Cryptococcus neoformans là một tác nhân gây bệnh nấm cơ hội gây ra viêm màng não do nấm men dạng nang. Điều thú vị là thành tế bào của C. neoformans có chứa chitosan, rất quan trọng đối với độc lực và sự tồn tại của nó trong vật chủ là động vật có vú. C. neoformans (H99) có ba chitin deacetylase (CDA), chuyển đổi chitin thành chitosan. Trong bài báo này, phân loại họ protein liên quan đến chitin (CRP) tập trung vào các CDA dạng nang được phân tích bằng phương pháp phát sinh loài, áp lực tiến hóa (dN/dS) và mô hình 3D. Cây phát sinh loài của 110 CRP cho thấy chúng có thể được chia thành hai nhánh, CRP I và II với giá trị khởi động (> 99%). Nhánh CRP I bao gồm năm nhóm (Nhóm 1-5) với tổng số 20 gen, trong khi nhánh CRP II bao gồm mười sáu nhóm (Nhóm 6-21) với tổng số 90 gen. CRP I chỉ bao gồm các CDA của nấm, bao gồm cả ba CDA của C. neoformans, trong khi CRP II bao gồm các CDA đa dạng từ nấm, vi khuẩn và amip, cùng với các protein họ estease carbohydrate 4 khác. Tất cả các CDA đều có peptide tín hiệu, ngoại trừ những CDA từ nhóm 11. Đáng chú ý, các CDA có vị trí O-glycosyl hóa được cho là có chứa mô típ neo glycosylphosphatidylinositol (GPI) cho CRP I hoặc miền liên kết chitin (CBD) cho CRP II. Sự bảo tồn tiến hóa này chỉ ra mạnh mẽ rằng sửa đổi O-glycosyl hóa và sự hiện diện của mô típ neo GPI hoặc miền liên kết chitin rất quan trọng để các CDA của nấm hoạt động hiệu quả ở bề mặt tế bào. Nghiên cứu này cho thấy các CDA của C. neoformans mang neo GPI đã tiến hóa khác biệt so với các CDA của nấm và vi khuẩn, cung cấp những hiểu biết mới về quá trình tiến hóa và phân loại họ CRP."} +{"idx": 1870664, "en": "In the article by Yu et al. published in Journal of Microbiology 2012; 50, 613-617, the figure 1 is unfortunately incorrect. The figure 1 should be corrected as below. The text of the figure legends and the article remains unchanged as they are correct.We apologize for any inconvenience that this may have caused.", "gemini": "Trong bài báo của Yu et al. được công bố trên Tạp chí Vi sinh năm 2012; 50, 613-617, hình 1 không may là không chính xác. Hình 1 nên được sửa lại như bên dưới. Văn bản chú thích hình và bài báo vẫn không thay đổi vì chúng là chính xác. Chúng tôi xin lỗi vì bất kỳ sự bất tiện nào mà điều này có thể gây ra."} +{"idx": 1870665, "en": "Enterovirus D68 (EVD68) is an emerging pathogen that recently caused a large worldwide outbreak of severe respiratory disease in children. However, the relationship between EVD68 and host cells remains unclear. Caspases are involved in cell death, immune response, and even viral production. We found that caspase-3 was activated during EVD68 replication to induce apoptosis. Caspase-3 inhibitor (Z-DEVD-FMK) inhibited viral production, protected host cells from the cytopathic effects of EVD68 infection, and prevented EVD68 from regulating the host cell cycle at G0/G1. Meanwhile, caspase-3 activator (PAC-1) increased EVD68 production. EVD68 infection therefore activates caspase-3 for virus production. This knowledge provides a potential direction for the prevention and treatment of disease related to EVD68.", "gemini": "Enterovirus D68 (EVD68) là một tác nhân gây bệnh mới nổi gần đây đã gây ra một đợt bùng phát bệnh hô hấp nghiêm trọng trên toàn thế giới ở trẻ em. Tuy nhiên, mối quan hệ giữa EVD68 và các tế bào vật chủ vẫn chưa rõ ràng. Caspase liên quan đến quá trình chết tế bào, đáp ứng miễn dịch và thậm chí là sản xuất vi-rút. Chúng tôi phát hiện ra rằng caspase-3 được kích hoạt trong quá trình sao chép EVD68 để gây ra apoptosis. Chất ức chế caspase-3 (Z-DEVD-FMK) ức chế sản xuất vi-rút, bảo vệ các tế bào vật chủ khỏi các tác động gây bệnh của nhiễm trùng EVD68 và ngăn EVD68 điều chỉnh chu kỳ tế bào vật chủ ở G0/G1. Trong khi đó, chất hoạt hóa caspase-3 (PAC-1) làm tăng sản xuất EVD68. Do đó, nhiễm trùng EVD68 kích hoạt caspase-3 để sản xuất vi-rút. Kiến thức này cung cấp một hướng tiềm năng cho việc phòng ngừa và điều trị bệnh liên quan đến EVD68."} +{"idx": 1870666, "en": "Exhibitionism has been viewed through many lenses, from the perspectives of sexual deviance, forensic psychiatry, psychopathology, psychological dynamics, feminism, behaviorism, and psychopharmacology. Starting from the description of one psychotherapy patient, the aim of this paper is to synthesize this disparate literature. The findings of the synthesis include an estimate of the lifetime male prevalence of exhibitionism, 2-4%, peaking in late adolescence. Insecure attachment, sexual abuse in childhood, substance abuse, and sexual dysfunction are acknowledged risk factors. Motives behind the act of genital exposure remain obscure, constructed of both sexual and non-sexual impulses. The usual response of women victims is alarm and disgust. Successful treatment relies on a strong therapeutic alliance with specific psychological and psychopharmacological interventions - comparative effectiveness not yet determined. In conclusion, precedents for exhibitionism vary. The frequency of the behavior usually wanes with age and, while exhibitionists may pose a risk to others, they usually do not. There is, as yet, no gold standard treatment; the recommendation for therapists is to respond to individual facets of the patient's circumstances and history.", "gemini": "Triển lãm đã được xem xét qua nhiều góc độ, từ quan điểm của lệch lạc tình dục, tâm thần pháp y, tâm lý bệnh học, động lực tâm lý, nữ quyền, hành vi học và dược lý tâm thần. Bắt đầu từ mô tả về một bệnh nhân tâm lý trị liệu, mục đích của bài báo này là tổng hợp các tài liệu rời rạc này. Những phát hiện của quá trình tổng hợp bao gồm ước tính tỷ lệ mắc bệnh triển lãm ở nam giới trong suốt cuộc đời là 2-4%, đạt đỉnh ở tuổi vị thành niên muộn. Sự gắn bó không an toàn, bị lạm dụng tình dục trong thời thơ ấu, lạm dụng chất gây nghiện và rối loạn chức năng tình dục được thừa nhận là các yếu tố nguy cơ. Động cơ đằng sau hành vi phơi bày bộ phận sinh dục vẫn còn mơ hồ, được cấu thành từ cả những xung lực tình dục và phi tình dục. Phản ứng thường gặp của phụ nữ nạn nhân là báo động và ghê tởm. Điều trị thành công dựa trên mối quan hệ trị liệu chặt chẽ với các can thiệp tâm lý và dược lý tâm thần cụ thể - hiệu quả so sánh vẫn chưa được xác định. Tóm lại, tiền lệ của chủ nghĩa triển lãm rất khác nhau. Tần suất của hành vi thường giảm dần theo tuổi tác và mặc dù những người theo chủ nghĩa triển lãm có thể gây nguy hiểm cho người khác, nhưng họ thường không làm vậy. Cho đến nay, vẫn chưa có phương pháp điều trị chuẩn mực; khuyến cáo dành cho các nhà trị liệu là phản ứng với các khía cạnh riêng lẻ về hoàn cảnh và tiền sử của bệnh nhân."} +{"idx": 1870667, "en": "INTRODUCTION Divergent objectives and narratives among members of a healthcare team may lead to suffering, underscoring the need to align patient care with the patient's self-identified priorities and goals. Shared decision making (SDM) with patients who may not be able to make healthcare decisions for themselves presents a unique challenge to healthcare providers, caregivers, and patients. Children and the elderly are two such groups where substituted decision making is often required. Family meetings, wherein stakeholders in a patient's care are gathered, present opportunities to align expectations and clinical goals. There is a clear need for a technique exploring all facets of the patient's story within the context of the biopsychosocial-spiritual model. We sought to promote narrative equity among stakeholders and maintain patient focus during family meetings. We describe the use of Mind Mapping in the family meeting to meet these objectives. METHODS Using two clinical scenarios, one involving a geriatric patient and another involving a pediatric patient, we describe the stepwise development of Mind Maps and how their use informed discussions among stakeholders in the family meeting. RESULTS Stakeholders found the Mind Maps easy to draw and helpful in eliciting their own priorities and preferences. Group exploration and refinement of the Mind Maps helped stakeholders to appreciate others' sometimes divergent perspectives, to ensure that the patient's voice was heard, and to ensure that care decisions were patient focused. DISCUSSION Mind Mapping was easily performed in two clinical scenarios, allowing the patient, family, and medical team to explore the biopsychosocial-spiritual model extensively, to appreciate each stakeholder's priorities, and to identify areas for further development. We have found that Mind Mapping helps define the 'topography' of relationships, prioritizes team discussions, finds shared interests in seemingly divergent objectives, and identifies which team member may best lead a discussion on a particular topic. CONCLUSION Mind Mapping may be a useful tool for family meetings, particularly for geriatric and pediatric patients with multiple stakeholders involved.", "gemini": "GIỚI THIỆU Các mục tiêu và tường thuật khác nhau giữa các thành viên trong nhóm chăm sóc sức khỏe có thể dẫn đến đau khổ, nhấn mạnh nhu cầu phải điều chỉnh việc chăm sóc bệnh nhân theo các ưu tiên và mục tiêu tự xác định của bệnh nhân. Quyết định chung (SDM) với những bệnh nhân có thể không tự đưa ra quyết định chăm sóc sức khỏe cho bản thân đặt ra một thách thức đặc biệt đối với các nhà cung cấp dịch vụ chăm sóc sức khỏe, người chăm sóc và bệnh nhân. Trẻ em và người cao tuổi là hai nhóm như vậy, nơi thường cần phải thay thế quyết định. Các cuộc họp gia đình, trong đó những người có liên quan đến việc chăm sóc bệnh nhân được tập hợp lại, tạo ra cơ hội để điều chỉnh các kỳ vọng và mục tiêu lâm sàng. Có một nhu cầu rõ ràng về một kỹ thuật khám phá mọi khía cạnh của câu chuyện bệnh nhân trong bối cảnh mô hình sinh học-tâm lý-xã hội. Chúng tôi tìm cách thúc đẩy sự công bằng trong tường thuật giữa các bên liên quan và duy trì sự tập trung vào bệnh nhân trong các cuộc họp gia đình. Chúng tôi mô tả việc sử dụng Bản đồ tư duy trong cuộc họp gia đình để đáp ứng các mục tiêu này. PHƯƠNG PHÁP Sử dụng hai kịch bản lâm sàng, một liên quan đến bệnh nhân lão khoa và một liên quan đến bệnh nhân nhi khoa, chúng tôi mô tả quá trình phát triển từng bước của Bản đồ tư duy và cách sử dụng của chúng thông báo các cuộc thảo luận giữa các bên liên quan trong cuộc họp gia đình. KẾT QUẢ Các bên liên quan thấy Bản đồ tư duy dễ vẽ và hữu ích trong việc nêu ra các ưu tiên và sở thích của chính họ. Việc khám phá và tinh chỉnh Bản đồ tư duy theo nhóm đã giúp các bên liên quan đánh giá cao quan điểm đôi khi khác biệt của người khác, đảm bảo rằng tiếng nói của bệnh nhân được lắng nghe và đảm bảo rằng các quyết định chăm sóc tập trung vào bệnh nhân. THẢO LUẬN Bản đồ tư duy đã được thực hiện dễ dàng trong hai kịch bản lâm sàng, cho phép bệnh nhân, gia đình và nhóm y tế khám phá sâu rộng mô hình sinh học-tâm lý-xã hội, đánh giá cao các ưu tiên của từng bên liên quan và xác định các lĩnh vực cần phát triển thêm. Chúng tôi đã phát hiện ra rằng Bản đồ tư duy giúp xác định 'địa hình' của các mối quan hệ, ưu tiên các cuộc thảo luận của nhóm, tìm ra sở thích chung trong các mục tiêu dường như khác biệt và xác định thành viên nào trong nhóm có thể dẫn dắt tốt nhất một cuộc thảo luận về một chủ đề cụ thể. KẾT LUẬN Bản đồ tư duy có thể là một công cụ hữu ích cho các cuộc họp gia đình, đặc biệt là đối với bệnh nhân lão khoa và nhi khoa có nhiều bên liên quan tham gia."} +{"idx": 1870668, "en": "Sepsis survivors present acute and long-term cognitive impairment and the pathophysiology of neurological dysfunction in sepsis involves microglial activation. Recently, the involvement of cytosolic receptors capable of forming protein complexes called inflammasomes have been demonstrated to perpetuate neuroinflammation. Thus, we investigated the involvement of the NLRP3 inflammasome activation on early and late brain changes in experimental sepsis. Two-month-old male Wistar rats were submitted to the sepsis model by cecal ligation and perforation (CLP group) or laparotomy only (sham group). Immediately after surgery, the animals received saline or NLRP3 inflammasome formation inhibitor (MCC950, 140 ng/kg) intracerebroventricularly. Prefrontal cortex and hippocampus were isolated for cytokine analysis, microglial and astrocyte activation, oxidative stress measurements, nitric oxide formation, and mitochondrial respiratory chain activity at 24 h after CLP. A subset of animals was followed for 10 days for survival assessment, and then behavioral tests were performed. The administration of MCC950 restored the elevation of IL-1β, TNF-α, IL-6, and IL-10 cytokine levels in the hippocampus. NLRP3 receptor levels increased in the prefrontal cortex and hippocampus at 24 h after sepsis, associated with microglial, but not astrocyte, activation. MCC950 reduced oxidative damage to lipids and proteins as well as preserved the activity of the enzyme SOD in the hippocampus. Mitochondrial respiratory chain activity presented variations in both structures studied. MCC950 reduced microglial activation, decreased acute neurochemical and behavioral alteration, and increased survival after experimental sepsis.", "gemini": "Những người sống sót sau nhiễm trùng huyết biểu hiện suy giảm nhận thức cấp tính và lâu dài và bệnh sinh của rối loạn chức năng thần kinh trong nhiễm trùng huyết liên quan đến hoạt hóa tế bào microglia. Gần đây, sự tham gia của các thụ thể tế bào chất có khả năng hình thành các phức hợp protein gọi là inflammasome đã được chứng minh là làm kéo dài tình trạng viêm thần kinh. Do đó, chúng tôi đã nghiên cứu sự tham gia của hoạt hóa NLRP3 inflammasome trên những thay đổi não sớm và muộn trong nhiễm trùng huyết thực nghiệm. Chuột Wistar đực hai tháng tuổi được đưa vào mô hình nhiễm trùng huyết bằng cách thắt và thủng manh tràng (nhóm CLP) hoặc chỉ phẫu thuật mở bụng (nhóm giả). Ngay sau phẫu thuật, các con vật được truyền dung dịch muối hoặc chất ức chế hình thành NLRP3 inflammasome (MCC950, 140 ng/kg) vào não thất. Vỏ não trước trán và hồi hải mã được phân lập để phân tích cytokine, hoạt hóa tế bào microglia và tế bào hình sao, đo lường stress oxy hóa, hình thành nitric oxit và hoạt động chuỗi hô hấp ty thể sau 24 giờ sau CLP. Một nhóm động vật được theo dõi trong 10 ngày để đánh giá khả năng sống sót, sau đó thực hiện các xét nghiệm hành vi. Việc sử dụng MCC950 đã khôi phục sự gia tăng nồng độ cytokine IL-1β, TNF-α, IL-6 và IL-10 trong hồi hải mã. Nồng độ thụ thể NLRP3 tăng ở vỏ não trước trán và hồi hải mã sau 24 giờ nhiễm trùng huyết, liên quan đến hoạt hóa tế bào microglia nhưng không phải tế bào hình sao. MCC950 làm giảm tổn thương oxy hóa đối với lipid và protein cũng như bảo tồn hoạt động của enzym SOD trong hồi hải mã. Hoạt động chuỗi hô hấp ty thể thể hiện sự thay đổi ở cả hai cấu trúc được nghiên cứu. MCC950 làm giảm hoạt hóa tế bào microglia, giảm thay đổi hành vi và thần kinh cấp tính và tăng khả năng sống sót sau nhiễm trùng huyết thực nghiệm."} +{"idx": 1870669, "en": "Pulmonary-renal syndrome refers to the combination of elevated plasma creatinine concentration and/or abnormal urinalysis with diffuse alveolar hemorrhage, and involves both an urgent diagnostic approach and care. We report the case of a 24-year-old man presenting with diffuse alveolar hemorrhage as well as a nephritic syndrome associating kidney failure, moderate hypertension, hematuria and selective glomerular proteinuria. The initial high suspicion of anti-glomerular basement membrane (GBM) disease or ANCA-associated vasculitis justified intravenous pulse-corticotherapy in association with plasma exchange. Renal biopsy was remarkable for an IgA nephropathy, lesions of active thrombotic microangiopathy (TMA) and a positive staining for complement factor C4d. Because anti-GBM and ANCA antibodies returned negative, plasma exchange was discontinued, but oral corticosteroids were maintained to prevent alveolar hemorrhage recurrence. In the absence of renal function recovery, hemodialysis was initiated. TMA lesions are frequently seen in IgA nephropathy and are associated with a poorer prognosis. Complement activation seems to be involved in the development of those lesions and contributes to disease progression. Conversely, alveolar hemorrhage in the setting of IgA nephropathy is uncommon. It is thought to result from non-specific mucosal hemorrhage, an immune complex mediated basement membrane damage and an IgA-mediated capillaritis against basement membrane antigens.", "gemini": "Hội chứng phổi-thận đề cập đến sự kết hợp của nồng độ creatinine huyết tương tăng cao và/hoặc xét nghiệm nước tiểu bất thường với xuất huyết phế nang lan tỏa, và liên quan đến cả phương pháp tiếp cận chẩn đoán và chăm sóc khẩn cấp. Chúng tôi báo cáo trường hợp của một người đàn ông 24 tuổi biểu hiện xuất huyết phế nang lan tỏa cũng như hội chứng viêm thận liên quan đến suy thận, tăng huyết áp vừa phải, tiểu máu và protein niệu cầu thận chọn lọc. Nghi ngờ ban đầu cao về bệnh màng đáy cầu thận (GBM) hoặc viêm mạch liên quan đến ANCA đã biện minh cho liệu pháp corticoid xung tĩnh mạch kết hợp với trao đổi huyết tương. Sinh thiết thận rất đáng chú ý đối với bệnh thận IgA, tổn thương vi mạch huyết khối tích cực (TMA) và nhuộm màu dương tính đối với yếu tố bổ sung C4d. Do kháng thể anti-GBM và ANCA cho kết quả âm tính, việc trao đổi huyết tương đã được ngừng lại, nhưng corticosteroid đường uống vẫn được duy trì để ngăn ngừa xuất huyết phế nang tái phát. Do không phục hồi chức năng thận nên đã bắt đầu lọc máu. Tổn thương TMA thường gặp ở bệnh thận IgA và có liên quan đến tiên lượng xấu hơn. Hoạt hóa bổ sung dường như có liên quan đến sự phát triển của những tổn thương đó và góp phần vào sự tiến triển của bệnh. Ngược lại, xuất huyết phế nang trong bối cảnh bệnh thận IgA không phổ biến. Người ta cho rằng nó là kết quả của xuất huyết niêm mạc không đặc hiệu, tổn thương màng đáy do phức hợp miễn dịch và viêm mao mạch do IgA chống lại các kháng nguyên màng đáy."} +{"idx": 1870670, "en": "Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are a relatively new class of antidiabetic drugs that in addition to emerging as an effective antihyperglycemic treatment have been shown to improve, in several trials, both renal and cardiovascular outcomes. In consideration of the renal site of action and the associated osmotic diuresis, a negative sodium balance has been postulated during SGLT2i administration. Actually, sodium and water depletion may contribute to some positive actions of SGLT2i but evidence is far from being conclusive and the real physiologic effects of SGLT2i on sodium remain largely unknown. Indeed, no study has yet investigated how SGLT2i change sodium balance in the long term and especially the pathways through which the natriuretic effect is expressed. Furthermore, several experimental studies have recently identified different pathways, not directly linked to tubular sodium handling, which could contribute to the renal and cardiovascular benefits associated with SGLT2i. This paper will review the evidence of SGLT2i action on sodium transporters, their off-target effects and their potential role on kidney protection.", "gemini": "Thuốc ức chế đồng vận chuyển natri-glucose 2 (SGLT2i) là một nhóm thuốc chống đái tháo đường tương đối mới, ngoài việc nổi lên như một phương pháp điều trị hạ đường huyết hiệu quả, đã được chứng minh là cải thiện cả kết quả về thận và tim mạch trong một số thử nghiệm. Khi xem xét vị trí tác dụng của thận và lợi niệu thẩm thấu liên quan, người ta đã đưa ra giả thuyết về sự cân bằng natri âm trong quá trình dùng SGLT2i. Trên thực tế, sự suy giảm natri và nước có thể góp phần vào một số tác động tích cực của SGLT2i nhưng bằng chứng vẫn chưa có tính thuyết phục và các tác động sinh lý thực sự của SGLT2i đối với natri vẫn phần lớn chưa được biết. Thật vậy, chưa có nghiên cứu nào điều tra cách SGLT2i thay đổi sự cân bằng natri trong thời gian dài và đặc biệt là các con đường mà qua đó tác dụng lợi natri được thể hiện. Hơn nữa, một số nghiên cứu thực nghiệm gần đây đã xác định các con đường khác nhau, không liên quan trực tiếp đến việc xử lý natri ở ống thận, có thể góp phần vào các lợi ích về thận và tim mạch liên quan đến SGLT2i. Bài báo này sẽ xem xét bằng chứng về tác dụng của SGLT2i đối với các chất vận chuyển natri, các tác dụng ngoài mục tiêu của chúng và vai trò tiềm năng của chúng đối với việc bảo vệ thận."} +{"idx": 1870671, "en": "INTRODUCTION In this edition, Szymuś et al. conducted a systematic review revealing sexual dysfunction to be more prevalent in patients with Huntington's Disease compared to controls. CLINICAL REFLECTIONS Sexual dysfunction in HD (SDHD) is common and significantly affects patient quality of life. Commonly used HD rating scales and treatment guidelines do not explicitly address SDHD, and research studies are limited by size and methodology. CLINICAL IMPLICATIONS It is important that validated sexual dysfunction screening tools be utilised in clinical and research settings.", "gemini": "GIỚI THIỆU Trong ấn bản này, Szymuś et al. đã tiến hành một đánh giá có hệ thống cho thấy rối loạn chức năng tình dục phổ biến hơn ở những bệnh nhân mắc bệnh Huntington so với nhóm đối chứng. SUY NGHĨ LÂM SÀNG Rối loạn chức năng tình dục ở HD (SDHD) phổ biến và ảnh hưởng đáng kể đến chất lượng cuộc sống của bệnh nhân. Các thang đánh giá HD thường dùng và hướng dẫn điều trị không đề cập rõ ràng đến SDHD và các nghiên cứu có giới hạn về quy mô và phương pháp luận. Ý NGHĨA LÂM SÀNG Điều quan trọng là các công cụ sàng lọc rối loạn chức năng tình dục đã được xác nhận phải được sử dụng trong các thiết lập lâm sàng và nghiên cứu."} +{"idx": 1870672, "en": "It is well known that the heart and kidney and their synergy is essential for hemodynamic homeostasis. Since the early XIX century it has been recognized that cardiovascular and renal diseases frequently coexist. In the nephrological field, while it is well accepted that renal diseases favor the occurrence of cardiovascular diseases, it is not always realized that cardiovascular diseases induce or aggravate renal dysfunctions, in this way further deteriorating cardiac function and creating a vicious circle. In the same clinical field, the role of venous congestion in the pathogenesis of renal dysfunction is at times overlooked. This review carefully quantifies the prevalence of chronic and acute kidney abnormalities in cardiovascular diseases, mainly heart failure, regardless of ejection fraction, and the consequences of renal abnormalities on both organs, making cardiovascular diseases a major risk factor for kidney diseases. In addition, with regard to pathophysiological aspects, we attempt to substantiate the major role of fluid overload and venous congestion, including renal venous hypertension, in the pathogenesis of acute and chronic renal dysfunction occurring in heart failure. Furthermore, we describe therapeutic principles to counteract the major pathophysiological abnormalities in heart failure complicated by renal dysfunction. Finally, we underline that the mild transient worsening of renal function after decongestive therapy is not usually associated with adverse prognosis. Accordingly, the coexistence of cardiovascular and renal diseases inevitably means mediating between preserving renal function and improving cardiac activity to reach a better outcome.", "gemini": "Người ta biết rằng tim và thận cùng sự hiệp đồng của chúng là thiết yếu đối với sự cân bằng động lực học. Từ đầu thế kỷ XIX, người ta đã nhận ra rằng bệnh tim mạch và bệnh thận thường cùng tồn tại. Trong lĩnh vực thận học, trong khi người ta chấp nhận rằng bệnh thận tạo điều kiện cho bệnh tim mạch xảy ra, thì người ta không phải lúc nào cũng nhận ra rằng bệnh tim mạch gây ra hoặc làm trầm trọng thêm các rối loạn chức năng thận, theo cách này làm suy giảm thêm chức năng tim và tạo ra một vòng luẩn quẩn. Trong cùng một lĩnh vực lâm sàng, vai trò của tình trạng sung huyết tĩnh mạch trong quá trình sinh bệnh của rối loạn chức năng thận đôi khi bị bỏ qua. Đánh giá này định lượng cẩn thận tỷ lệ mắc các bất thường về thận cấp tính và mãn tính trong các bệnh tim mạch, chủ yếu là suy tim, bất kể phân suất tống máu, và hậu quả của các bất thường về thận đối với cả hai cơ quan, khiến bệnh tim mạch trở thành một yếu tố nguy cơ chính đối với bệnh thận. Ngoài ra, liên quan đến các khía cạnh bệnh sinh lý, chúng tôi cố gắng chứng minh vai trò chính của tình trạng quá tải dịch và sung huyết tĩnh mạch, bao gồm cả tăng huyết áp tĩnh mạch thận, trong quá trình sinh bệnh của rối loạn chức năng thận cấp tính và mãn tính xảy ra trong suy tim. Hơn nữa, chúng tôi mô tả các nguyên tắc điều trị để chống lại các bất thường về bệnh sinh lý chính trong suy tim có biến chứng rối loạn chức năng thận. Cuối cùng, chúng tôi nhấn mạnh rằng tình trạng suy giảm chức năng thận thoáng qua nhẹ sau liệu pháp chống sung huyết thường không liên quan đến tiên lượng xấu. Theo đó, sự cùng tồn tại của bệnh tim mạch và bệnh thận chắc chắn có nghĩa là phải cân nhắc giữa việc bảo tồn chức năng thận và cải thiện hoạt động của tim để đạt được kết quả tốt hơn."} +{"idx": 1870673, "en": "The spatial habitat heterogeneity hypothesis posits that habitat complexity increases the abundance and diversity of species. In tropical forests, lianas add substantial habitat heterogeneity and complexity throughout the vertical forest profile, which may maintain animal abundance and diversity. The effects of lianas on tropical animal communities, however, remain poorly understood. We propose that lianas have a positive effect on animals by enhancing habitat complexity. Lianas may have a particularly strong influence on the forest bird community, providing nesting substrate, protection from predators, and nutrition (food). Understory insectivorous birds, which forage for insects that specialize on lianas, may particularly benefit. Alternatively, it is possible that lianas have a negative effect on forest birds by increasing predator abundances and providing arboreal predators with travel routes with easy access to bird nests. We tested the spatial habitat heterogeneity hypothesis on bird abundance and diversity by removing lianas, thus reducing forest complexity, using a large-scale experimental approach in a lowland tropical forest in the Republic of Panama. We found that removing lianas decreased total bird abundance by 78.4% and diversity by 77.4% after 8 months, and by 40.0% and 51.7%, respectively, after 20 months. Insectivorous bird abundance and diversity 8 months after liana removal were 91.8% and 89.5% lower, respectively, indicating that lianas positively influence insectivorous birds. The effects of liana removal persisted longer for insectivorous birds than other birds, with 77.3% lower abundance and 76.2% lower diversity after 20 months. Liana removal also altered bird community composition, creating two distinct communities in the control and removal plots, with disproportionate effects on insectivores. Our findings demonstrate that lianas have a strong positive influence on the bird community, particularly for insectivorous birds in the forest understory. Lianas may maintain bird abundance and diversity by increasing habitat complexity, habitat heterogeneity, and resource availability.", "gemini": "Giả thuyết về tính không đồng nhất của môi trường sống không gian cho rằng tính phức tạp của môi trường sống làm tăng sự phong phú và đa dạng của các loài. Ở các khu rừng nhiệt đới, dây leo làm tăng đáng kể tính không đồng nhất và độ phức tạp của môi trường sống trên khắp cấu hình rừng theo chiều dọc, điều này có thể duy trì sự phong phú và đa dạng của động vật. Tuy nhiên, ảnh hưởng của dây leo đối với các quần xã động vật nhiệt đới vẫn chưa được hiểu rõ. Chúng tôi đề xuất rằng dây leo có tác động tích cực đến động vật bằng cách tăng cường tính phức tạp của môi trường sống. Dây leo có thể có ảnh hưởng đặc biệt mạnh đến quần xã chim rừng, cung cấp giá thể làm tổ, bảo vệ khỏi động vật ăn thịt và dinh dưỡng (thức ăn). Các loài chim ăn côn trùng tầng dưới, chuyên kiếm ăn các loài côn trùng chuyên sống trên dây leo, có thể đặc biệt được hưởng lợi. Mặt khác, cũng có khả năng dây leo có tác động tiêu cực đến các loài chim rừng bằng cách làm tăng số lượng động vật ăn thịt và cung cấp cho động vật ăn thịt trên cây các tuyến đường di chuyển dễ dàng tiếp cận tổ chim. Chúng tôi đã kiểm tra giả thuyết về tính không đồng nhất của môi trường sống không gian đối với sự phong phú và đa dạng của các loài chim bằng cách loại bỏ dây leo, do đó làm giảm độ phức tạp của rừng, sử dụng phương pháp tiếp cận thử nghiệm quy mô lớn trong một khu rừng nhiệt đới đất thấp ở Cộng hòa Panama. Chúng tôi phát hiện ra rằng việc loại bỏ dây leo làm giảm tổng số lượng chim xuống 78,4% và sự đa dạng xuống 77,4% sau 8 tháng và lần lượt là 40,0% và 51,7% sau 20 tháng. Sự phong phú và đa dạng của các loài chim ăn côn trùng sau 8 tháng kể từ khi loại bỏ dây leo lần lượt thấp hơn 91,8% và 89,5%, cho thấy dây leo có ảnh hưởng tích cực đến các loài chim ăn côn trùng. Các tác động của việc loại bỏ dây leo kéo dài hơn đối với các loài chim ăn côn trùng so với các loài chim khác, với sự phong phú thấp hơn 77,3% và sự đa dạng thấp hơn 76,2% sau 20 tháng. Việc loại bỏ dây leo cũng làm thay đổi thành phần quần xã chim, tạo ra hai quần xã riêng biệt trong các ô kiểm soát và loại bỏ, với những tác động không cân xứng đến các loài ăn côn trùng. Những phát hiện của chúng tôi chứng minh rằng dây leo có ảnh hưởng tích cực mạnh mẽ đến quần xã chim, đặc biệt là đối với các loài chim ăn côn trùng ở tầng dưới của rừng. Dây leo có thể duy trì sự phong phú và đa dạng của các loài chim bằng cách tăng tính phức tạp của môi trường sống, tính không đồng nhất của môi trường sống và tính sẵn có của nguồn tài nguyên."} +{"idx": 1870674, "en": "MTG8 (RUNX1T1) is a fusion partner of AML1 (RUNX1) in the leukemic chromosome translocation t(8;21). The AML1-MTG8 fusion gene encodes a chimeric transcription factor. One of the highly conserved domains of MTG8 is TAFH which possesses homology with human TAF4 [TATA-box binding protein-associated factor]. To obtain specific inhibitors of the AML1-MTG8 fusion protein, we isolated RNA aptamers against the MTG8 TAFH domain using systematic evolution of ligands by exponential enrichment. All TAF aptamers contained guanine-rich sequences. Analyses of a TAF aptamer by NMR, CD, and mutagenesis revealed that it forms a parallel G-quadruplex structure in the presence of K+ . Furthermore, the aptamer could bind to the AML1-MTG8 fusion protein and dissociate the AML1-MTG8/DNA complex, suggesting that it can inhibit the dominant negative effects of AML1-MTG8 against normal AML1 function and serve as a potential therapeutic agent for leukemia.", "gemini": "MTG8 (RUNX1T1) là một đối tác hợp nhất của AML1 (RUNX1) trong quá trình chuyển đoạn nhiễm sắc thể t(8;21) gây bệnh bạch cầu. Gen hợp nhất AML1-MTG8 mã hóa một yếu tố phiên mã dị hợp tử. Một trong những miền được bảo tồn cao của MTG8 là TAFH có tính đồng đẳng với TAF4 của người [yếu tố liên kết với protein liên kết hộp TATA]. Để có được các chất ức chế đặc hiệu của protein hợp nhất AML1-MTG8, chúng tôi đã phân lập các phân tử aptane RNA chống lại miền TAFH của MTG8 bằng cách sử dụng phương pháp tiến hóa có hệ thống của các phối tử theo sự làm giàu theo cấp số nhân. Tất cả các phân tử aptane TAF đều chứa các trình tự giàu guanine. Các phân tích về một phân tử aptane TAF bằng NMR, CD và đột biến cho thấy nó tạo thành cấu trúc G-tứ liên song khi có mặt K+. Hơn nữa, phân tử aptane có thể liên kết với protein hợp nhất AML1-MTG8 và phân ly phức hợp AML1-MTG8/DNA, cho thấy rằng nó có thể ức chế các tác động tiêu cực trội của AML1-MTG8 đối với chức năng AML1 bình thường và đóng vai trò là một tác nhân điều trị tiềm năng cho bệnh bạch cầu."} +{"idx": 1870675, "en": "OBJECTIVES This study aimed to investigate whether the number of vaginal births a woman has alters the association between symptoms and signs of pelvic organ prolapse. METHODS In this retrospective study, we investigated data on 1748 women seen between April 2012 and January 2016. To elucidate whether the number of vaginal births is a confounder of this relationship, we used receiver operating characteristic statistics to investigate the association between symptoms and signs of prolapse in women of different vaginal parity. RESULTS A higher number of vaginal births was associated with a higher likelihood of symptoms of prolapse for any given degree of prolapse. Multivariate modeling, however, showed this confounding effect to be due to higher vaginal parity in older women (P < .001). Receiver operating characteristic curves obtained for the association between symptoms and signs of prolapse were near identical regardless of whether vaginal parity was included in the modeling. This was true for both clinical and imaging assessments of prolapse. CONCLUSIONS Although vaginal childbirth is associated with symptoms of prolapse, the number of vaginal births does not alter the relationship between symptoms and signs of prolapse among individuals presenting to a tertiary care center for evaluation. Previously published limits for \"normal\" pelvic organ mobility can be used irrespective of vaginal parity.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm mục đích điều tra xem số lần sinh ngả âm đạo của một phụ nữ có làm thay đổi mối liên quan giữa các triệu chứng và dấu hiệu của sa tạng chậu hay không. PHƯƠNG PHÁP Trong nghiên cứu hồi cứu này, chúng tôi đã điều tra dữ liệu về 1748 phụ nữ được khám từ tháng 4 năm 2012 đến tháng 1 năm 2016. Để làm sáng tỏ liệu số lần sinh ngả âm đạo có phải là yếu tố gây nhiễu trong mối quan hệ này hay không, chúng tôi đã sử dụng thống kê đặc tính hoạt động của máy thu để điều tra mối liên quan giữa các triệu chứng và dấu hiệu sa ở những phụ nữ có số lần sinh ngả âm đạo khác nhau. KẾT QUẢ Số lần sinh ngả âm đạo càng nhiều thì khả năng xuất hiện các triệu chứng sa càng cao đối với bất kỳ mức độ sa nào. Tuy nhiên, mô hình đa biến cho thấy hiệu ứng gây nhiễu này là do số lần sinh ngả âm đạo cao hơn ở những phụ nữ lớn tuổi (P <0,001). Các đường cong đặc tính hoạt động của máy thu thu được cho mối liên quan giữa các triệu chứng và dấu hiệu sa gần như giống hệt nhau bất kể số lần sinh ngả âm đạo có được đưa vào mô hình hay không. Điều này đúng đối với cả đánh giá lâm sàng và đánh giá hình ảnh về tình trạng sa. KẾT LUẬN Mặc dù sinh ngả âm đạo có liên quan đến các triệu chứng sa, nhưng số lần sinh ngả âm đạo không làm thay đổi mối quan hệ giữa các triệu chứng và dấu hiệu sa ở những cá nhân đến trung tâm chăm sóc sức khỏe chuyên khoa để đánh giá. Các giới hạn đã công bố trước đây đối với khả năng vận động \"bình thường\" của các cơ quan vùng chậu có thể được sử dụng bất kể số lần sinh ngả âm đạo."} +{"idx": 1870676, "en": "OBJECTIVE To describe the outcome of small ruminants treated with unilateral and bilateral mastectomy by using three surgical techniques. STUDY DESIGN Retrospective study. ANIMALS Twenty-five small ruminants (24 goats and one sheep). METHODS Medical records of animals that underwent mastectomy between November 1, 2002, and May 1, 2019, were reviewed. Follow-up information was obtained by telephone questionnaire with owners. Signalment, surgical data, intraoperative and postoperative complications, bacterial culture results, histopathologic diagnoses, short- and long-term outcomes, and other procedures performed were recorded. RESULT Procedures consisted of six unilateral (with an elliptical incision) and 19 total (with inverted cloverleaf or elliptical skin incisions) mastectomies. All animals survived to hospital discharge. Intraoperative complications included contamination of the surgical site with mammary-gland fluid, hemorrhage, and difficulty dissecting skin from the mammary gland. Postoperative complications included seroma formation (7/25), surgical-site infection (5/25), and dehiscence of the skin incision (3/25). Mammary neoplasia was diagnosed in seven of 15 animals with histopathologic examination. No association was detected between surgical technique, diagnosis of neoplasia, and long-term outcome. Overall, client satisfaction was high. CONCLUSION Mastectomy was effective at removing abnormally enlarged udders secondary to chronic mastitis, inappropriate lactation, idiopathic causes, or neoplasia and was associated with a low rate of complications in small ruminants. CLINICAL SIGNIFICANCE Unilateral mastectomy with an elliptical skin incision or total mastectomy, preferably with inverted cloverleaf skin incision, may be indicated to remove diseased mammary tissue in small ruminants and can result in long-term survival with low morbidity and cosmetically pleasing results.", "gemini": "MỤC TIÊU Mô tả kết quả của các động vật nhai lại nhỏ được điều trị cắt bỏ vú đơn phương và song phương bằng ba kỹ thuật phẫu thuật. THIẾT KẾ NGHIÊN CỨU Nghiên cứu hồi cứu. ĐỘNG VẬT Hai mươi lăm động vật nhai lại nhỏ (24 con dê và một con cừu). PHƯƠNG PHÁP Hồ sơ bệnh án của những động vật đã cắt bỏ vú từ ngày 1 tháng 11 năm 2002 đến ngày 1 tháng 5 năm 2019 đã được xem xét lại. Thông tin theo dõi được thu thập bằng bảng câu hỏi qua điện thoại với chủ sở hữu. Các thông tin về tín hiệu, dữ liệu phẫu thuật, các biến chứng trong và sau phẫu thuật, kết quả nuôi cấy vi khuẩn, chẩn đoán mô bệnh học, kết quả trong thời gian ngắn và dài hạn cùng các thủ thuật khác được thực hiện đã được ghi lại. KẾT QUẢ Các thủ thuật bao gồm sáu lần cắt bỏ vú đơn phương (với một vết rạch hình elip) và 19 lần cắt bỏ vú toàn bộ (với các vết rạch trên da hình lá cỏ ba lá ngược hoặc hình elip). Tất cả các động vật đều sống sót để xuất viện. Các biến chứng trong khi phẫu thuật bao gồm nhiễm bẩn vị trí phẫu thuật bằng dịch tuyến vú, xuất huyết và khó bóc tách da khỏi tuyến vú. Các biến chứng sau phẫu thuật bao gồm hình thành dịch thanh (7/25), nhiễm trùng tại vị trí phẫu thuật (5/25) và vết rạch trên da bị hở (3/25). Trong số 15 động vật được xét nghiệm mô bệnh học, có bảy động vật được chẩn đoán mắc bệnh tân sinh tuyến vú. Không phát hiện thấy mối liên quan nào giữa kỹ thuật phẫu thuật, chẩn đoán tân sinh và kết quả lâu dài. Nhìn chung, sự hài lòng của khách hàng là cao. KẾT LUẬN Cắt bỏ vú là phương pháp hiệu quả để loại bỏ các tuyến vú bị phì đại bất thường thứ phát do viêm vú mạn tính, tiết sữa không thích hợp, các nguyên nhân vô căn hoặc tân sinh và có tỷ lệ biến chứng thấp ở động vật nhai lại nhỏ. Ý NGHĨA LÂM SÀNG Cắt bỏ vú đơn phương với vết rạch trên da hình elip hoặc cắt bỏ vú toàn bộ, tốt nhất là với vết rạch trên da hình lá cỏ ba lá ngược, có thể được chỉ định để loại bỏ mô vú bị bệnh ở động vật nhai lại nhỏ và có thể dẫn đến sự sống sót lâu dài với tỷ lệ mắc bệnh thấp và kết quả thẩm mỹ đẹp."} +{"idx": 1870677, "en": "BACKGROUND AND AIM Although the gut microbiome of patients with ulcerative colitis (UC) has been characterized, no study has characterized the gut microbiome in acute severe colitis (ASC). We compared the gut microbiome of patients with UC, ASC, and healthy controls (HCs). METHODS Patients with mild to moderate UC (n = 24), ASC (n = 19 with 21 episodes) and HCs (n = 50) were recruited prospectively. A 16SrDNA amplicon approach was used to explore gut microbial diversity and taxonomic repertoires. UC was diagnosed using European Crohn's and Colitis Organization guidelines, and ASC was diagnosed using Truelove and Witts' criteria. RESULTS The normalized alpha diversity was significantly lower in ASC than mild-moderately active UC (P < 0.05) or HC (P < 0.001). The gut microbiome in ASC was highly unstable, as characterized by high intracohort variation (analyzed using J-divergence measure), which was significantly greater than in UC or HC. On principal coordinate analysis, the microbiome of HC and UC were similar, with the ASC cohort being distinct from both. Comparison of ranked abundances identified four distinct clusters of genera (G1, G2, G3, and G4), with specific trends in their abundance across three groups: G1/G2A clusters had the least, whereas G3 had the highest abundance in the ASC cohort. CONCLUSIONS Gut microbial diversity is lower in ASC than mild-moderate UC or HCs. Gut microbiome composition is increasingly unstable in ASC, with a distinct abundance of specific genera varying between HCs and ASC. Mild-moderate UC lies within the spectrum.", "gemini": "BỐI CẢNH VÀ MỤC TIÊU Mặc dù hệ vi sinh đường ruột của bệnh nhân viêm loét đại tràng (UC) đã được mô tả, nhưng chưa có nghiên cứu nào mô tả hệ vi sinh đường ruột trong viêm đại tràng cấp tính nặng (ASC). Chúng tôi đã so sánh hệ vi sinh đường ruột của bệnh nhân UC, ASC và đối chứng khỏe mạnh (HC). PHƯƠNG PHÁP Bệnh nhân mắc UC nhẹ đến trung bình (n = 24), ASC (n = 19 với 21 đợt) và HC (n = 50) đã được tuyển dụng theo hướng nghiên cứu. Một phương pháp khuếch đại 16SrDNA đã được sử dụng để khám phá sự đa dạng của vi khuẩn đường ruột và các kho vi khuẩn phân loại. UC được chẩn đoán bằng hướng dẫn của Tổ chức Crohn và Viêm đại tràng Châu Âu, và ASC được chẩn đoán bằng tiêu chuẩn của Truelove và Witts. KẾT QUẢ Độ đa dạng alpha chuẩn hóa thấp hơn đáng kể ở ASC so với UC hoạt động nhẹ-trung bình (P <0,05) hoặc HC (P <0,001). Hệ vi sinh đường ruột ở ASC rất không ổn định, được đặc trưng bởi sự biến đổi cao trong nhóm (được phân tích bằng phép đo độ phân kỳ J), lớn hơn đáng kể so với UC hoặc HC. Trên phân tích tọa độ chính, hệ vi sinh của HC và UC tương tự nhau, trong khi nhóm ASC khác biệt so với cả hai. So sánh các mức độ xếp hạng đã xác định bốn nhóm chi riêng biệt (G1, G2, G3 và G4), với các xu hướng cụ thể về mức độ phong phú của chúng trong ba nhóm: các nhóm G1/G2A có mức độ thấp nhất, trong khi G3 có mức độ phong phú cao nhất trong nhóm ASC. KẾT LUẬN Độ đa dạng của vi khuẩn đường ruột thấp hơn ở ASC so với UC nhẹ-trung bình hoặc HC. Thành phần hệ vi sinh đường ruột ngày càng không ổn định ở ASC, với sự phong phú riêng biệt của các chi cụ thể khác nhau giữa HC và ASC. UC nhẹ-trung bình nằm trong phổ."} +{"idx": 1870678, "en": "Instructions highlighting that backward conditional stimuli (CSs) stop unconditional stimuli (USs) result in their acquiring valence opposite to that of the US on explicit measures of valence. We assessed whether such instructions would influence startle blink modulation in the same way. Two groups were presented with concurrent forward and backward evaluative conditioning (CS-US-CS) using cartoon aliens as CSs, and pleasant, neutral, and unpleasant sounds as USs. Startle magnitude was measured during conditioning and valence ratings were assessed after conditioning. Participants in the \"start-stop\" instructions group (n = 41) were instructed to learn whether CSs started or stopped US presentations, while participants in the \"observe\" instructions group (n = 41) were told to pay attention to the stimuli as they would be asked questions about them after the experiment. In the start-stop instructions group backward CSs paired with positive USs were rated as less pleasant than backward CSs paired with neutral and negative USs (contrast effect), whereas ratings of backward CSs did not differ in the observe instructions group. Startle magnitude was larger during backward CSs paired with positive USs in comparison to CSs paired with neutral or negative USs in both instruction groups. Startle blink modulation was unaffected by instructions, suggesting that startle indexes the emotional state at the time of probe presentation rather than CS valence based on propositional information about the function of the CS.", "gemini": "Hướng dẫn nêu bật rằng các kích thích có điều kiện ngược (CS) dừng các kích thích vô điều kiện (US) dẫn đến việc chúng có được giá trị trái ngược với US trên các biện pháp giá trị rõ ràng. Chúng tôi đánh giá xem các hướng dẫn như vậy có ảnh hưởng đến điều chế nháy giật sợ theo cách tương tự hay không. Hai nhóm được trình bày với điều kiện đánh giá đồng thời tiến và lùi (CS-US-CS) sử dụng người ngoài hành tinh hoạt hình làm CS và âm thanh dễ chịu, trung tính và khó chịu làm US. Cường độ giật mình được đo trong quá trình điều hòa và đánh giá giá trị được đánh giá sau khi điều hòa. Những người tham gia trong nhóm hướng dẫn \"bắt đầu dừng\" (n = 41) được hướng dẫn tìm hiểu xem CS bắt đầu hay dừng các bài thuyết trình của Hoa Kỳ, trong khi những người tham gia trong nhóm hướng dẫn \"quan sát\" (n = 41) được yêu cầu chú ý đến các kích thích vì họ sẽ được hỏi các câu hỏi về chúng sau thí nghiệm. Trong nhóm hướng dẫn bắt đầu dừng, CS ngược được ghép nối với US tích cực được đánh giá là ít dễ chịu hơn so với CS ngược được ghép nối với US trung tính và tiêu cực (hiệu ứng tương phản), trong khi xếp hạng CS ngược không khác nhau trong nhóm hướng dẫn quan sát. Cường độ giật mình lớn hơn trong quá trình CS ngược được ghép nối với US tích cực so với CS được ghép nối với US trung tính hoặc tiêu cực trong cả hai nhóm hướng dẫn. Điều chế nháy giật sợ không bị ảnh hưởng bởi các hướng dẫn, điều này cho thấy giật mình chỉ ra trạng thái cảm xúc tại thời điểm trình bày đầu dò chứ không phải giá trị CS dựa trên thông tin đề xuất về chức năng của CS."} +{"idx": 1870679, "en": "Invited for the cover of this issue is Ken Kamikawa and co-workers at Osaka Prefecture University and RIKEN Center for Sustainable Resource Science. The image depicts an S-shaped double azahelicene capturing the palladium in a trans-chelating fashion. Read the full text of the article at 10.1002/chem.202002405.", "gemini": "Được mời lên trang bìa của số báo này là Ken Kamikawa và các cộng sự tại Đại học Osaka Prefecture và Trung tâm Khoa học Tài nguyên Bền vững RIKEN. Hình ảnh mô tả một azahelicene đôi hình chữ S bắt giữ palladium theo kiểu chelat chuyển tiếp. Đọc toàn văn bài báo tại 10.1002/chem.202002405."} +{"idx": 1870680, "en": "OBJECTIVES The aim of the present prospective study was to assess the risk for disease recurrence following surgical therapy of peri-implantitis. MATERIAL & METHODS 73 patients (130 implants) treated surgically for peri-implantitis were examined at 1 and 5 years after therapy. The primary outcome was recurrence/progression of disease defined as any of the following events: (a) bone loss >1.0 mm, (b) surgical retreatment, (c) implant removal/loss after year 1. Patient- and implant-related parameters as well as 1-year outcomes were evaluated as potential predictors through multiple logistic regression analysis. RESULTS 57 implants (44%) displayed recurrence/progression of peri-implantitis during follow-up. Among these, 27 implants were removed. Residual deep probing pocket depth (≥6 mm; odds ratio 7.4; 95% confidence interval 2.8-19.3) and reduced marginal bone level (OR 1.4; 95%CI 1.1-1.7) at 1 year after surgery constituted risk factors for recurrence/progression of disease. Furthermore, implants with modified surfaces were at higher risk than implants with non-modified surfaces (OR 5.1; 95%CI 1.6-16.5). CONCLUSION Implants with (a) residual deep probing pocket depth, (b) reduced marginal bone level, or (c) modified surfaces following surgical therapy of peri-implantitis present with increased risk for recurrence/progression.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu triển vọng hiện tại là đánh giá nguy cơ tái phát bệnh sau phẫu thuật điều trị viêm quanh implant. VẬT LIỆU & PHƯƠNG PHÁP 73 bệnh nhân (130 implant) được điều trị phẫu thuật vì viêm quanh implant được kiểm tra sau 1 và 5 năm điều trị. Kết quả chính là tái phát/tiến triển của bệnh được định nghĩa là bất kỳ sự kiện nào sau đây: (a) mất xương> 1,0 mm, (b) phẫu thuật điều trị lại, (c) loại bỏ/mất implant sau năm 1. Các thông số liên quan đến bệnh nhân và implant cũng như kết quả sau 1 năm được đánh giá là các yếu tố dự báo tiềm năng thông qua phân tích hồi quy logistic đa biến. KẾT QUẢ 57 implant (44%) biểu hiện tái phát/tiến triển của viêm quanh implant trong quá trình theo dõi. Trong số đó, 27 implant đã được loại bỏ. Độ sâu túi thăm dò sâu còn lại (≥6 mm; tỷ lệ chênh lệch 7,4; khoảng tin cậy 95% 2,8-19,3) và mức xương biên giảm (OR 1,4; 95%CI 1,1-1,7) sau 1 năm phẫu thuật là các yếu tố nguy cơ tái phát/tiến triển của bệnh. Hơn nữa, các implant có bề mặt được sửa đổi có nguy cơ cao hơn so với các implant có bề mặt không được sửa đổi (OR 5,1; 95%CI 1,6-16,5). KẾT LUẬN Các implant có (a) độ sâu túi thăm dò sâu còn lại, (b) mức xương biên giảm hoặc (c) bề mặt được sửa đổi sau phẫu thuật điều trị viêm quanh implant có nguy cơ tái phát/tiến triển cao hơn."} +{"idx": 1870681, "en": "BACKGROUND Maternal iodine requirements increase during pregnancy to supply thyroid hormones critical for fetal neurodevelopment. Iodine insufficiency may result in poorer cognitive or child educational outcomes but current evidence is sparse and inconsistent. OBJECTIVES To quantify the association between maternal iodine status and child educational outcomes. METHODS Urinary iodine concentrations (UIC) and iodine/creatinine ratios (I:Cr) were measured in 6971 mothers at 26-28 weeks' gestation participating in the Born in Bradford cohort. Maternal iodine status was examined in relation to child school achievement (early years foundation stage (EYFS), phonics, and Key Stage 1 (KS1)), other learning outcomes, social and behavioural difficulties, and sensorimotor control in 5745 children aged 4-7 years. RESULTS Median (interquartile range) UIC was 76 µg/L (46, 120), and I:Cr was 83 µg/g (59, 121). Overall, there was no strong or consistent evidence to support associations between UIC or I:Cr and neurodevelopmental outcomes. For instance, predicted EYFS and phonics scores (primary outcomes) at the 25th vs 75th I:Cr percentiles (99% confidence intervals) were similar, with no evidence of associations: EYFS scores were 32 (99% CI 31, 33) and 33 (99% CI 32, 34), and phonics scores were 34 (99% CI 33, 35) and 35 (99% CI 34, 36), respectively. CONCLUSIONS In the largest single study of its kind, there was little evidence of detrimental neurodevelopmental outcomes in children born to pregnant women with iodine insufficiency as defined by World Health Organization-outlined thresholds. Alternative functional biomarkers for iodine status in pregnancy and focused assessment of other health outcomes may provide additional insight.", "gemini": "BỐI CẢNH Nhu cầu i-ốt của bà mẹ tăng lên trong thời kỳ mang thai để cung cấp hormone tuyến giáp rất quan trọng cho sự phát triển thần kinh của thai nhi. Sự thiếu hụt i-ốt có thể dẫn đến kết quả nhận thức hoặc giáo dục của trẻ kém hơn nhưng bằng chứng hiện tại còn ít và không nhất quán. MỤC TIÊU Để định lượng mối liên quan giữa tình trạng i-ốt của bà mẹ và kết quả giáo dục của trẻ. PHƯƠNG PHÁP Nồng độ i-ốt trong nước tiểu (UIC) và tỷ lệ i-ốt/creatinin (I:Cr) được đo ở 6971 bà mẹ ở tuần thai thứ 26-28 tham gia nhóm nghiên cứu Sinh ra ở Bradford. Tình trạng i-ốt của bà mẹ được kiểm tra liên quan đến thành tích học tập của trẻ (giai đoạn nền tảng những năm đầu (EYFS), ngữ âm và Khóa học chính 1 (KS1)), các kết quả học tập khác, khó khăn về mặt xã hội và hành vi và kiểm soát cảm biến vận động ở 5745 trẻ em từ 4-7 tuổi. KẾT QUẢ Dải trung vị (khoảng tứ phân vị) của UIC là 76 µg/L (46, 120) và I:Cr là 83 µg/g (59, 121). Nhìn chung, không có bằng chứng mạnh mẽ hoặc nhất quán nào hỗ trợ cho mối liên quan giữa UIC hoặc I:Cr và kết quả phát triển thần kinh. Chẳng hạn, điểm số EYFS và ngữ âm dự đoán (kết quả chính) ở các phần trăm thứ 25 so với 75 của I:Cr (khoảng tin cậy 99%) là tương tự, không có bằng chứng về mối liên quan: điểm EYFS là 32 (99% CI 31, 33) và 33 (99% CI 32, 34), và điểm ngữ âm lần lượt là 34 (99% CI 33, 35) và 35 (99% CI 34, 36). KẾT LUẬN Trong nghiên cứu đơn lẻ lớn nhất cùng loại, có rất ít bằng chứng về kết quả phát triển thần kinh bất lợi ở trẻ em sinh ra từ những bà mẹ mang thai bị thiếu hụt i-ốt theo định nghĩa là ngưỡng do Tổ chức Y tế Thế giới nêu. Các dấu ấn sinh học chức năng thay thế cho tình trạng i-ốt trong thai kỳ và đánh giá tập trung vào các kết quả sức khỏe khác có thể cung cấp thêm thông tin chi tiết."} +{"idx": 1870682, "en": "BACKGROUND All antigens described in the KN blood group system are located in the long homologous repeat D (LHR-D) of complement receptor 1 (CR1). While there have been reports that some sera react only with the long homologous repeat C (LHR-C), the antigens in LHR-C are unknown. STUDY DESIGN AND METHODS Recombinant LHR-C and LHR-D were used to identify antibodies directed against LHR-C of CR1, into which a point mutation was introduced to characterize the underlying blood group antigens. In addition, database studies to define haplotypes of CR1 were performed. RESULTS Several antisera were identified that were specific against CR1 p.1208His and against CR1 p.1208Arg, located in LHR-C. Fifteen KN haplotypes were found in the Ensembl genome browser. It was shown that due to a linkage disequilibrium anti-CR1 p.1208His may be mistaken for anti-KCAM. CONCLUSION A novel antithetical KN blood group antigen pair was found at position p.1208 of CR1, for which the names DACY and YCAD are proposed. Antibodies against these two novel antigens seem to contribute to more than a quarter of all KN sera in Europe.", "gemini": "BỐI CẢNH Tất cả các kháng nguyên được mô tả trong hệ nhóm máu KN đều nằm trong đoạn lặp đồng dạng dài D (LHR-D) của thụ thể bổ thể 1 (CR1). Mặc dù có báo cáo rằng một số huyết thanh chỉ phản ứng với đoạn lặp đồng dạng dài C (LHR-C), nhưng các kháng nguyên trong LHR-C vẫn chưa được biết. THIẾT KẾ VÀ PHƯƠNG PHÁP NGHIÊN CỨU LHR-C tái tổ hợp và LHR-D được sử dụng để xác định các kháng thể hướng chống lại LHR-C của CR1, trong đó một đột biến điểm đã được đưa vào để đặc trưng cho các kháng nguyên nhóm máu cơ bản. Ngoài ra, các nghiên cứu cơ sở dữ liệu để xác định kiểu gen của CR1 đã được thực hiện. KẾT QUẢ Một số huyết thanh đã được xác định là đặc hiệu chống lại CR1 p.1208His và chống lại CR1 p.1208Arg, nằm trong LHR-C. Mười lăm kiểu gen KN đã được tìm thấy trong trình duyệt bộ gen Ensembl. Người ta đã chứng minh rằng do sự liên kết không cân bằng, anti-CR1 p.1208His có thể bị nhầm lẫn với anti-KCAM. KẾT LUẬN Một cặp kháng nguyên nhóm máu KN đối lập mới đã được tìm thấy ở vị trí p.1208 của CR1, trong đó các tên DACY và YCAD được đề xuất. Các kháng thể chống lại hai kháng nguyên mới này dường như chiếm hơn một phần tư tất cả huyết thanh KN ở Châu Âu."} +{"idx": 1870683, "en": "BACKGROUND The COVID-19 pandemic has resulted in massive disruptions to society, to the economy, and to daily life. Some people may turn to alcohol to cope with stress during the pandemic, which may put them at risk for heavy drinking and alcohol-related harms. Research is needed to identify factors that are relevant for coping-motivated drinking during these extraordinary circumstances to inform interventions. This study provides an empirical examination of coping motive pathways to alcohol problems during the early stages of the COVID-19 pandemic. METHODS Participants (N = 320; 54.7% male; mean age of 32 years) were Canadian adult drinkers who completed an online survey assessing work- and home-related factors, psychological factors, and alcohol-related outcomes over the past 30 days, covering a time period beginning within 1 month of the initiation of the COVID-19 emergency response. RESULTS The results of a theory-informed path model showed that having at least 1 child under the age of 18, greater depression, and lower social connectedness each predicted unique variance in past 30-day coping motives, which in turn predicted increased past 30-day alcohol use (controlling for pre-COVID-19 alcohol use reported retrospectively). Income loss was associated with increased alcohol use, and living alone was associated with increased solitary drinking (controlling for pre-COVID-19 levels), but these associations were not mediated by coping motives. Increased alcohol use, increased solitary drinking, and greater coping motives for drinking were all independently associated with past 30-day alcohol problems, and indirect paths to alcohol problems from having children at home, depression, social connectedness, income loss, and living alone were all supported. CONCLUSIONS Findings provide insight into coping-motivated drinking early in the COVID-19 pandemic and highlight the need for longitudinal research to establish longer term outcomes of drinking to cope during the pandemic.", "gemini": "BỐI CẢNH Đại dịch COVID-19 đã gây ra những gián đoạn lớn đối với xã hội, nền kinh tế và cuộc sống hàng ngày. Một số người có thể chuyển sang dùng rượu để đối phó với căng thẳng trong thời kỳ đại dịch, điều này có thể khiến họ có nguy cơ uống nhiều rượu và gây hại liên quan đến rượu. Cần nghiên cứu để xác định các yếu tố có liên quan đến việc uống rượu vì động cơ đối phó trong những hoàn cảnh đặc biệt này để thông tin cho các biện pháp can thiệp. Nghiên cứu này cung cấp một cuộc kiểm tra thực nghiệm về các con đường động cơ đối phó với các vấn đề về rượu trong giai đoạn đầu của đại dịch COVID-19. PHƯƠNG PHÁP Những người tham gia (N = 320; 54,7% nam; tuổi trung bình 32) là những người uống rượu trưởng thành ở Canada đã hoàn thành một cuộc khảo sát trực tuyến đánh giá các yếu tố liên quan đến công việc và gia đình, các yếu tố tâm lý và kết quả liên quan đến rượu trong 30 ngày qua, bao gồm một khoảng thời gian bắt đầu trong vòng 1 tháng kể từ khi bắt đầu phản ứng khẩn cấp với COVID-19. KẾT QUẢ Kết quả của một mô hình đường dẫn được thông tin theo lý thuyết cho thấy việc có ít nhất 1 đứa trẻ dưới 18 tuổi, trầm cảm nhiều hơn và kết nối xã hội thấp hơn đều dự đoán được sự khác biệt duy nhất về động cơ đối phó trong 30 ngày qua, từ đó dự đoán việc sử dụng rượu trong 30 ngày qua tăng lên (kiểm soát việc sử dụng rượu trước COVID-19 được báo cáo hồi cứu). Mất thu nhập có liên quan đến việc sử dụng rượu tăng lên và sống một mình có liên quan đến việc uống rượu một mình tăng lên (kiểm soát mức độ trước COVID-19), nhưng những mối liên quan này không được thúc đẩy bởi động cơ đối phó. Việc sử dụng rượu tăng lên, uống rượu một mình tăng lên và động cơ đối phó lớn hơn để uống rượu đều có liên quan độc lập với các vấn đề về rượu trong 30 ngày qua và các con đường gián tiếp dẫn đến các vấn đề về rượu từ việc có con ở nhà, trầm cảm, kết nối xã hội, mất thu nhập và sống một mình đều được hỗ trợ. KẾT LUẬN Các phát hiện cung cấp cái nhìn sâu sắc về việc uống rượu vì động cơ đối phó vào giai đoạn đầu của đại dịch COVID-19 và nêu bật nhu cầu nghiên cứu theo chiều dọc để thiết lập các kết quả lâu dài hơn của việc uống rượu để đối phó trong thời kỳ đại dịch."} +{"idx": 1870684, "en": "The COVID-19 pandemic has demonstrated the fragility of clinic-based care for Parkinson's disease and other movement disorders. In response to the virus, many clinics across the world abruptly closed their doors to persons with Parkinson's disease. Fortunately, a niche care model, telemedicine-first described in this journal a generation ago-emerged as the dominant means of providing care. As we adjust to a new normal, we should focus future care not on clinics but on patients. Their needs, guided by clinicians, should determine how care is delivered, whether in the clinic, at home, remotely, or by some combination. Within this patient-centered approach, telemedicine is an attractive care option but not a complete replacement for in-person consultations, which are valuable for specific problems and for those who have access. Now that many clinicians and patients have gained exposure to telemedicine, we can better appreciate its advantages (eg, convenience) and disadvantages (eg, restricted examination). We can also create a new future that utilizes the Internet, video conferencing, smartphones, and sensors. This future will bring many clinicians to one patient, connect individual experts to countless patients, use widely available devices to facilitate diagnosis, and apply novel technologies to measure the disease in new ways. These approaches, which extend to education and research, enable a future where we can care for anyone anywhere and will help us stem the tide of Parkinson's disease. © 2020 International Parkinson and Movement Disorder Society.", "gemini": "Đại dịch COVID-19 đã chứng minh sự mong manh của dịch vụ chăm sóc dựa trên phòng khám đối với bệnh Parkinson và các rối loạn vận động khác. Để ứng phó với loại vi-rút này, nhiều phòng khám trên khắp thế giới đã đột ngột đóng cửa đối với những người mắc bệnh Parkinson. May mắn thay, một mô hình chăm sóc thích hợp, y học từ xa - được mô tả đầu tiên trên tạp chí này cách đây một thế hệ - đã nổi lên như một phương tiện chăm sóc chính. Khi chúng ta thích nghi với trạng thái bình thường mới, chúng ta nên tập trung chăm sóc trong tương lai không phải vào các phòng khám mà là vào bệnh nhân. Nhu cầu của họ, được hướng dẫn bởi các bác sĩ lâm sàng, sẽ quyết định cách cung cấp dịch vụ chăm sóc, cho dù tại phòng khám, tại nhà, từ xa hay kết hợp một số cách. Trong cách tiếp cận lấy bệnh nhân làm trung tâm này, y học từ xa là một lựa chọn chăm sóc hấp dẫn nhưng không phải là sự thay thế hoàn toàn cho các buổi tư vấn trực tiếp, có giá trị đối với các vấn đề cụ thể và đối với những người có thể tiếp cận. Bây giờ, khi nhiều bác sĩ lâm sàng và bệnh nhân đã tiếp xúc với y học từ xa, chúng ta có thể đánh giá cao hơn những ưu điểm (ví dụ: sự tiện lợi) và nhược điểm (ví dụ: hạn chế khám) của nó. Chúng ta cũng có thể tạo ra một tương lai mới sử dụng Internet, hội nghị truyền hình, điện thoại thông minh và cảm biến. Tương lai này sẽ đưa nhiều bác sĩ lâm sàng đến với một bệnh nhân, kết nối các chuyên gia riêng lẻ với vô số bệnh nhân, sử dụng các thiết bị có sẵn rộng rãi để tạo điều kiện chẩn đoán và áp dụng các công nghệ mới để đo lường bệnh theo những cách mới. Những cách tiếp cận này, mở rộng đến giáo dục và nghiên cứu, cho phép một tương lai mà chúng ta có thể chăm sóc cho bất kỳ ai ở bất kỳ đâu và sẽ giúp chúng ta ngăn chặn làn sóng bệnh Parkinson. © 2020 Hội rối loạn vận động và Parkinson quốc tế."} +{"idx": 1870685, "en": "Previously, we introduced DFT-D3(BJ)ωB97X-V and ωB97M-V functionals and assessed them for the GMTKN55 database [Najibi and Goerigk, J Chem. Theory Comput. 2018, 14, 5725]. In this study, we present DFT-D4 damping parameters to build the DFT-D4 counterparts of these functionals and assess these in comparison. We extend our analysis beyond GMTKN55 and especially turn our attention to enzymatically catalyzed and metal-organic reactions. We find that B97M-D4 is now the second-best performing meta-generalized-gradient approximation functional for the GMTKN55 database and it can provide noticeably better organometallic reaction energies compared to B97M-D3(BJ). Moreover, the aforementioned DFT-D3(BJ)-based functionals have not been thoroughly assessed for geometries and herein we close this gap by analyzing geometries of noncovalently bound dimers and trimers, peptide conformers, water hexamers and transition-metal complexes. We find that several of the B97(M)-based methods-particularly the DFT-D4 versions-surpass the accuracy of previously studied methods for peptide conformer, water hexamer, and transition-metal complex geometries, making them safe-to-use, cost-efficient alternatives to the original methods. The DFT-D4 variants can be easily used with ORCA4.1 and above.", "gemini": "Trước đây, chúng tôi đã giới thiệu các hàm DFT-D3(BJ)ωB97X-V và ωB97M-V và đánh giá chúng cho cơ sở dữ liệu GMTKN55 [Najibi và Goerigk, J Chem. Lý thuyết Comput. 2018, 14, 5725]. Trong nghiên cứu này, chúng tôi trình bày các tham số giảm chấn DFT-D4 để xây dựng các đối tác DFT-D4 của các hàm này và đánh giá chúng để so sánh. Chúng tôi mở rộng phân tích của mình ra ngoài GMTKN55 và đặc biệt chú ý đến các phản ứng xúc tác bằng enzym và phản ứng hữu cơ kim loại. Chúng tôi thấy rằng B97M-D4 hiện là hàm xấp xỉ gradien tổng quát hóa tốt thứ hai cho cơ sở dữ liệu GMTKN55 và nó có thể cung cấp năng lượng phản ứng organometallic tốt hơn đáng kể so với B97M-D3(BJ). Hơn nữa, các hàm dựa trên DFT-D3(BJ) đã nói ở trên chưa được đánh giá kỹ lưỡng về mặt hình học và tại đây chúng tôi thu hẹp khoảng cách này bằng cách phân tích hình học của các dimer và trimer liên kết không cộng hóa trị, cấu tử peptit, hexamer nước và phức chất kim loại chuyển tiếp. Chúng tôi thấy rằng một số phương pháp dựa trên B97(M) - đặc biệt là các phiên bản DFT-D4 - vượt qua độ chính xác của các phương pháp đã nghiên cứu trước đây đối với cấu tử peptit, hexamer nước và hình học phức hợp kim loại chuyển tiếp, khiến chúng trở thành những phương pháp thay thế an toàn, hiệu quả về chi phí cho các phương pháp ban đầu. Các biến thể DFT-D4 có thể dễ dàng sử dụng với ORCA4.1 trở lên."} +{"idx": 1870686, "en": "The diagnosis of ovarian torsion is challenging and relies mostly on morphologic findings. Occasionally, women or children with acute pelvic pain who have undergone an initial ultrasound (US) evaluation with results interpreted as negative for ovarian torsion will return with recurrent or increasing pain, prompting an US reevaluation. The flipped ovary sign refers to a demonstrable change in the orientation of the ovary on follow-up US examinations, recognized by changing positions of ovarian landmarks established by follicles, cysts, or masses. This sign is valuable for identifying ovarian torsion in these patients, even in the absence of classic morphologic or Doppler features of ovarian torsion.", "gemini": "Việc chẩn đoán xoắn buồng trứng rất khó khăn và chủ yếu dựa vào các phát hiện về mặt hình thái. Đôi khi, phụ nữ hoặc trẻ em bị đau vùng chậu cấp tính đã trải qua đánh giá siêu âm (US) ban đầu với kết quả được giải thích là âm tính với xoắn buồng trứng sẽ quay trở lại với cơn đau tái phát hoặc tăng lên, thúc đẩy việc đánh giá lại bằng US. Dấu hiệu buồng trứng lật đề cập đến sự thay đổi có thể chứng minh được về hướng của buồng trứng trên các lần kiểm tra US tiếp theo, được nhận biết bằng cách thay đổi vị trí của các mốc buồng trứng được thiết lập bởi các nang, u nang hoặc khối u. Dấu hiệu này có giá trị để xác định xoắn buồng trứng ở những bệnh nhân này, ngay cả khi không có các đặc điểm hình thái hoặc Doppler cổ điển của xoắn buồng trứng."} +{"idx": 1870687, "en": "BACKGROUND Atypical cutaneous lymphoid infiltrates are challenging lesions in dermatopathology. We present a summary of the literature regarding kappa and lambda immunohistochemistry (IHC) and in situ hybridization (ISH) in the evaluation of atypical cutaneous or mucosal lymphoid infiltrates. METHODS Relevant articles from 1967 to 2018 in the English language were identified and summarized. In the absence of larger studies, case series of n ≥ 3 were included. RESULTS Sixty-three articles assessing kappa and lambda IHC and/or ISH were identified. Most focused on marginal zone lymphomas. Other lymphomas included follicle center lymphoma, diffuse large B-cell lymphoma, chronic lymphocytic leukemia/small lymphocytic lymphoma, mantle cell lymphoma, lymphoplasmacytic lymphoma, plasmablastic lymphoma, multiple myeloma, monoclonal gammopathy of undetermined significance, and polyneuropathy, organomegaly, endocrinopathy, monoclonal protein, skin changes (POEMS). Non-neoplastic lesions included reactive lymphoid hyperplasia, cutaneous plasmacytosis, connective tissue disease, IgG4-related disease, acrodermatitis chronic atrophicans, Zoon balanitis, dermatitides, and infiltrates around epithelial dysplasias/neoplasias. CONCLUSION Kappa and lambda IHC and ISH are useful tools in the evaluation of cutaneous B-cell lymphomas and plasma cell neoplasms. The literature supports that the detection of light-chain restriction by IHC and ISH is one of the most useful findings in the differential diagnosis of reactive lymphoid hyperplasia vs B-cell lymphoma with plasmacytic differentiation.", "gemini": "LÝ LỊCH Các thâm nhiễm lympho bào dạng da không điển hình là những tổn thương khó khăn trong bệnh lý da. Chúng tôi trình bày tóm tắt các tài liệu về miễn dịch mô hóa học kappa và lambda (IHC) và lai ghép tại chỗ (ISH) trong đánh giá các thâm nhiễm lympho bào dạng da hoặc niêm mạc không điển hình. PHƯƠNG PHÁP Các bài báo có liên quan từ năm 1967 đến năm 2018 bằng tiếng Anh đã được xác định và tóm tắt. Trong trường hợp không có các nghiên cứu lớn hơn, các loạt ca có n ≥ 3 đã được đưa vào. KẾT QUẢ Đã xác định được sáu mươi ba bài báo đánh giá IHC kappa và lambda và/hoặc ISH. Hầu hết tập trung vào các u lympho vùng biên. Các u lympho khác bao gồm u lympho trung tâm nang, u lympho tế bào B lớn lan tỏa, bệnh bạch cầu lymphocytic mạn tính/u lympho lymphocytic nhỏ, u lympho tế bào vỏ, u lympho tế bào lympho plasma, u lympho plasma bào, đa u tủy, bệnh gammopathy đơn dòng chưa xác định ý nghĩa và bệnh đa dây thần kinh, to các cơ quan, bệnh nội tiết, protein đơn dòng, thay đổi da (POEMS). Các tổn thương không phải tân sinh bao gồm tăng sản lympho phản ứng, bệnh huyết tương da, bệnh mô liên kết, bệnh liên quan đến IgG4, viêm da teo mạn tính, viêm quy đầu Zoon, viêm da và thâm nhiễm xung quanh loạn sản/tân sinh biểu mô. KẾT LUẬN IHC kappa và lambda và ISH là những công cụ hữu ích trong đánh giá các u lympho tế bào B dạng da và tân sinh tế bào plasma. Các tài liệu hỗ trợ rằng phát hiện hạn chế chuỗi nhẹ bằng IHC và ISH là một trong những phát hiện hữu ích nhất trong chẩn đoán phân biệt tăng sản lympho phản ứng với u lympho tế bào B có biệt hóa tế bào tương bào."} +{"idx": 1870688, "en": "Many global changes take the form of resource enhancements that have potential to transform multiple aspects of ecosystems from slower to faster cycling, including a suite of both above- and belowground variables. We developed a novel analytic approach to measure integrated ecosystem responses to resource-enhancing global changes, and how such whole ecosystem slow-to-fast transitions are linked to diversity and exotic invasions in real-world ecosystems. We asked how 5-yr experimental rainfall and nutrient enhancements in a natural grassland system affected 16 ecosystem functions, pools, and stoichiometry variables considered to indicate slow vs. fast cycling. We combined these metrics into a novel index we termed \"slow-fast multifunctionality\" and assessed its relationship to plant community diversity and exotic plant dominance. Nutrient and rainfall addition interacted to affect average slow-fast multifunctionality. Nutrient addition alone pushed the system toward faster cycling, but this effect weakened with the joint addition of rainfall and nutrients. Variables associated with soil nutrient pools and cycling most strongly contributed to this antagonistic interaction. Nutrient and water addition together, respectively, had additive or synergistic effects on plant trait composition and productivity, demonstrating divergence of above- and belowground ecosystem responses. Our novel metric of faster cycling was strongly associated with decreased plant species richness and increased exotic species dominance. These results demonstrate the breadth of interacting community and ecosystem changes that ensue when resource limitation is relaxed.", "gemini": "Nhiều thay đổi toàn cầu diễn ra dưới dạng tăng cường tài nguyên có tiềm năng biến đổi nhiều khía cạnh của hệ sinh thái từ chu trình chậm hơn sang chu trình nhanh hơn, bao gồm một loạt các biến số trên và dưới mặt đất. Chúng tôi đã phát triển một phương pháp phân tích mới để đo lường các phản ứng của hệ sinh thái tích hợp đối với những thay đổi toàn cầu làm tăng cường tài nguyên và cách thức chuyển đổi từ chậm sang nhanh của toàn bộ hệ sinh thái như vậy có liên quan đến sự đa dạng và xâm lược kỳ lạ trong các hệ sinh thái thực tế. Chúng tôi đã đặt câu hỏi rằng lượng mưa thử nghiệm trong 5 năm và sự gia tăng chất dinh dưỡng trong một hệ thống đồng cỏ tự nhiên ảnh hưởng như thế nào đến 16 chức năng hệ sinh thái, nhóm và biến số về tỷ lượng được coi là chỉ ra chu trình chậm so với nhanh. Chúng tôi đã kết hợp các số liệu này thành một chỉ số mới mà chúng tôi gọi là \"đa chức năng chậm-nhanh\" và đánh giá mối quan hệ của nó với sự đa dạng của quần xã thực vật và sự thống trị của các loài thực vật kỳ lạ. Thêm chất dinh dưỡng và lượng mưa tương tác để ảnh hưởng đến đa chức năng chậm-nhanh trung bình. Chỉ riêng việc bổ sung chất dinh dưỡng đã đẩy hệ thống hướng đến chu trình nhanh hơn, nhưng hiệu ứng này yếu đi khi bổ sung đồng thời lượng mưa và chất dinh dưỡng. Các biến số liên quan đến nhóm chất dinh dưỡng trong đất và chu trình đóng góp mạnh nhất vào tương tác đối kháng này. Việc bổ sung chất dinh dưỡng và nước tương ứng có tác dụng cộng gộp hoặc hiệp đồng đối với thành phần và năng suất của đặc điểm thực vật, chứng tỏ sự khác biệt trong phản ứng của hệ sinh thái trên và dưới mặt đất. Chỉ số mới về chu trình nhanh hơn của chúng tôi có liên quan chặt chẽ đến việc giảm độ phong phú của các loài thực vật và tăng sự thống trị của các loài kỳ lạ. Những kết quả này chứng minh được chiều rộng của các thay đổi tương tác của quần xã và hệ sinh thái xảy ra khi sự hạn chế về tài nguyên được nới lỏng."} +{"idx": 1870689, "en": "This study focused on fundamental data acquisition parameter selection for a benchtop nuclear magnetic resonance (NMR) system with continuous flow, applicable for reaction monitoring. The effect of flow rate on the mixing behaviors within a flow cell was observed, along with an exponential decay relationship between flow rate and the apparent spin-lattice relaxation time (T1*) of benzaldehyde. We also monitored sensitivity (as determined by signal-to-noise ratios; SNRs) under various flow rates, analyte concentrations, and temperatures of the analyte flask. Results suggest that a maximum SNR can be achieved with low to medium flow rates and higher analyte concentrations. This was consistent with data collected with parameters that promote either slow or fast data acquisition. We further consider the effect of these conditions on the analyte's residence time, T1*, and magnetic field inhomogeneity that is a product of continuous flow. Altogether, our results demonstrate how fundamental acquisition parameters can be manipulated to achieve optimal data acquisition in continuous-flow NMR systems.", "gemini": "Nghiên cứu này tập trung vào việc lựa chọn tham số thu nhận dữ liệu cơ bản cho hệ thống cộng hưởng từ hạt nhân (NMR) trên băng ghế với dòng chảy liên tục, có thể áp dụng để theo dõi phản ứng. Tác động của tốc độ dòng chảy đối với hành vi trộn bên trong tế bào dòng chảy đã được quan sát, cùng với mối quan hệ phân rã theo cấp số nhân giữa tốc độ dòng chảy và thời gian giãn spin-lưới biểu kiến (T1*) của benzaldehyde. Chúng tôi cũng theo dõi độ nhạy (được xác định bằng tỷ số tín hiệu trên nhiễu; SNR) ở nhiều tốc độ dòng chảy, nồng độ chất phân tích và nhiệt độ của bình đựng chất phân tích khác nhau. Kết quả cho thấy có thể đạt được SNR tối đa với tốc độ dòng chảy từ thấp đến trung bình và nồng độ chất phân tích cao hơn. Điều này phù hợp với dữ liệu thu thập được với các thông số thúc đẩy thu nhận dữ liệu chậm hoặc nhanh. Hơn nữa, chúng tôi xem xét tác động của các điều kiện này đến thời gian lưu trú của chất phân tích, T1* và độ không đồng nhất của từ trường là sản phẩm của dòng chảy liên tục. Nhìn chung, kết quả của chúng tôi chứng minh cách các tham số thu nhận cơ bản có thể được điều chỉnh để đạt được mức thu nhận dữ liệu tối ưu trong các hệ thống NMR dòng chảy liên tục."} +{"idx": 1870690, "en": "Naringinase was mainly obtained by microbial fermentation, and mutagenesis was a major way for obtaining excellent mutants. The aim of this study was to screen out a high naringinase yielding mutant to enhance the potential application value of its industrialization and compare the effects of different mutagenic methods on the enzyme activity of the strain. A novel producing naringinase strain, Aspergillus tubingensis MN589840, was isolated from mildewed pomelo peel, later subjected to mutagenesis including UV, ARTP and UV-ARTP. After five rounds iterative mutagenesis, the mutants U1, A6 and UA13 were screened out with 1448·49, 1848·71, 2475·16 U mg-1 enzyme activity, the naringinase productivity raised by 79·08, 123·56 and 206%, respectively. In addition, the naringinase activity of three mutants rose after each round of iterative mutagenesis. These results indicated that the mutagenesis efficiency of UV-ARTP was higher than that of single ARTP, and both are better than UV. In summary, the iterative UV-ARTP mutagenesis is an effective strategy for screening high naringinase-producing strains.", "gemini": "Naringinase chủ yếu thu được bằng phương pháp lên men vi sinh vật, và gây đột biến là một cách chính để thu được các đột biến tuyệt vời. Mục đích của nghiên cứu này là sàng lọc ra một đột biến naringinase có năng suất cao để nâng cao giá trị ứng dụng tiềm năng của quá trình công nghiệp hóa và so sánh tác động của các phương pháp gây đột biến khác nhau đối với hoạt động của enzyme của chủng. Một chủng sản xuất naringinase mới, Aspergillus tubingensis MN589840, đã được phân lập từ vỏ bưởi bị mốc, sau đó được gây đột biến bao gồm UV, ARTP và UV-ARTP. Sau năm vòng gây đột biến lặp lại, các đột biến U1, A6 và UA13 đã được sàng lọc với hoạt động của enzyme lần lượt là 1448,49, 1848,71, 2475,16 U mg-1, năng suất naringinase tăng lần lượt là 79,08, 123,56 và 206%. Ngoài ra, hoạt động naringinase của ba đột biến tăng lên sau mỗi vòng gây đột biến lặp lại. Những kết quả này chỉ ra rằng hiệu quả gây đột biến của UV-ARTP cao hơn so với ARTP đơn lẻ và cả hai đều tốt hơn UV. Tóm lại, gây đột biến UV-ARTP lặp lại là một chiến lược hiệu quả để sàng lọc các chủng sản xuất naringinase cao."} +{"idx": 1870691, "en": "BACKGROUND AND AIM The incidence of non-alcoholic steatohepatitis (NASH)-related hepatocellular carcinoma (HCC) is progressively increasing. However, the pathophysiology and etiology of NASH progression to HCC are unknown. We hypothesized that steatosis was the key factor in NASH-related hepatocarcinogenesis and aimed to evaluate the effects of long-term liver X receptor (LXR) agonist stimulation on hepatic steatosis induced by a high-fat diet and oxidative stress. METHODS We used an LXR agonist (T0901317) and CCl4 to induce hepatic steatosis and oxidative stress, respectively. C57BL/6 mice fed with a high-fat diet were treated with either T0901317 + CCl4 (T09 + CCl4 group) or CCl4 alone (CCl4 group). T0901317 (2.5 mg/kg) and CCl4 (0.1 mL/kg) were intraperitoneally administered twice weekly for 24 weeks. RESULTS The liver-to-body weight ratio was significantly higher in the T09 + CCl4 group than in the CCl4 group. Mice in the T09 + CCl4 group exhibited abnormal lipid metabolism and NASH-like histopathological features. Additionally, all mice in the T09 + CCl4 group developed liver tumors diagnosed as well-differentiated HCC. The genes identified via microarray analysis were related to NASH and HCC development. CONCLUSIONS By combining long-term LXR agonist stimulation with oxidative stress and a high-fat diet, we successfully reproduced liver conditions in mice similar to those in humans with NASH and progression to HCC. Our results provide new insight into NASH-related HCC progression and therapy.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Tỷ lệ mắc ung thư biểu mô tế bào gan (HCC) liên quan đến viêm gan nhiễm mỡ không do rượu (NASH) đang tăng dần. Tuy nhiên, bệnh sinh và nguyên nhân của sự tiến triển của NASH thành HCC vẫn chưa được biết rõ. Chúng tôi giả định rằng tình trạng gan nhiễm mỡ là yếu tố chính trong quá trình gây ung thư biểu mô gan liên quan đến NASH và nhằm đánh giá tác động của việc kích thích thụ thể X gan (LXR) kéo dài đối với tình trạng gan nhiễm mỡ do chế độ ăn nhiều chất béo và stress oxy hóa gây ra. PHƯƠNG PHÁP Chúng tôi đã sử dụng chất chủ vận LXR (T0901317) và CCl4 để gây ra tình trạng gan nhiễm mỡ và stress oxy hóa. Chuột C57BL/6 được cho ăn chế độ ăn nhiều chất béo được điều trị bằng T0901317 + CCl4 (nhóm T09 + CCl4) hoặc chỉ CCl4 (nhóm CCl4). T0901317 (2,5 mg/kg) và CCl4 (0,1 mL/kg) được tiêm phúc mạc hai lần một tuần trong 24 tuần. KẾT QUẢ Tỷ lệ gan trên trọng lượng cơ thể cao hơn đáng kể ở nhóm T09 + CCl4 so với nhóm CCl4. Chuột trong nhóm T09 + CCl4 biểu hiện chuyển hóa lipid bất thường và các đặc điểm mô bệnh học giống NASH. Ngoài ra, tất cả chuột trong nhóm T09 + CCl4 đều phát triển khối u gan được chẩn đoán là HCC biệt hóa tốt. Các gen được xác định thông qua phân tích microarray có liên quan đến sự phát triển của NASH và HCC. KẾT LUẬN Bằng cách kết hợp kích thích chất chủ vận LXR kéo dài với stress oxy hóa và chế độ ăn nhiều chất béo, chúng tôi đã tái tạo thành công tình trạng gan ở chuột tương tự như ở người mắc NASH và tiến triển thành HCC. Kết quả của chúng tôi cung cấp cái nhìn sâu sắc mới về sự tiến triển và điều trị HCC liên quan đến NASH."} +{"idx": 1870692, "en": "Induction of broad Th1 cellular immune responses and cytokines is crucial characteristics for vaccines against intracellular infections such as hepatitis C virus (HCV). Plants (especially oilseed tissues) and plant-immunomodulators (like oil bodies) offer cost-effective and scalable possibilities for the production of immunologically relevant and safe vaccine antigens and adjuvants, respectively. Herein, we provide data of the murine immunization by transgenic canola oilseed-derived HCV core protein (HCVcp) soluble extract (TSE) and Escherichia coli- derived rHCVcp in combination with Canola oil bodies (oil) compared to that of the Freund's (FA) adjuvant. Mice immunized by TSE+ oil developed both strong humeral (IgG) and Th1-biased cellular responses, manifested by high levels of IFN-γ and lower IgG1/IgG2a ratio and IL-4 secretion. Results of the intracellular cytokine staining indicated that TSE+ oil immunization in mice triggered both CD4+ and CD8+ T cells to release IFN-γ, while CD4+ cells were mostly triggered when FA was used. Analyses by qRT-PCR indicated that a combination of rHCVcp/TSE with oil body induced high levels of IL-10 cytokines compared to that of the FA adjuvant. These characteristics are important properties for the design of an HCV vaccine candidate and indicate the potential of Canola-derived antigen and oil bodies in addressing these concerns.", "gemini": "Việc tạo ra các phản ứng miễn dịch tế bào Th1 rộng và cytokine là đặc điểm quan trọng đối với vắc-xin chống lại các bệnh nhiễm trùng trong tế bào như virus viêm gan C (HCV). Thực vật (đặc biệt là các mô hạt có dầu) và các chất điều hòa miễn dịch thực vật (như các hạt dầu) cung cấp khả năng hiệu quả về chi phí và khả năng mở rộng để sản xuất các kháng nguyên vắc-xin có liên quan đến miễn dịch và an toàn tương ứng. Trong bài báo này, chúng tôi cung cấp dữ liệu về quá trình miễn dịch ở chuột bằng chiết xuất hòa tan protein lõi HCV có nguồn gốc từ hạt cải dầu chuyển gen (TSE) và rHCVcp có nguồn gốc từ Escherichia coli kết hợp với các hạt dầu cải dầu (dầu) so với tá dược Freund (FA). Chuột được miễn dịch bằng TSE + dầu đã phát triển cả phản ứng tế bào mạnh về dịch thể (IgG) và tế bào thiên về Th1, biểu hiện ở mức cao của IFN-γ và tỷ lệ IgG1/IgG2a và tiết IL-4 thấp hơn. Kết quả nhuộm màu cytokine nội bào chỉ ra rằng quá trình miễn dịch TSE + dầu ở chuột kích hoạt cả tế bào T CD4+ và CD8+ giải phóng IFN-γ, trong khi tế bào CD4+ chủ yếu được kích hoạt khi sử dụng FA. Các phân tích bằng qRT-PCR chỉ ra rằng sự kết hợp của rHCVcp/TSE với hạt dầu gây ra mức cytokine IL-10 cao so với tá dược FA. Những đặc điểm này là đặc tính quan trọng để thiết kế ứng viên vắc-xin HCV và chỉ ra tiềm năng của kháng nguyên có nguồn gốc từ cải dầu và các hạt dầu trong việc giải quyết những mối quan tâm này."} +{"idx": 1870693, "en": "SARS-CoV-2 is responsible for a new infectious disease (COVID-19) in which individuals can either remain asymptomatic or progress from mild to severe clinical conditions including acute respiratory distress syndrome and multiple organ failure. The immune mechanisms that potentially orchestrate the pathology in SARS-CoV-2 infection are complex and only partially understood. There is still paucity of data on the features of myeloid cells involved in this viral infection. For this reason, we investigated the different activation status profiles and the subset distribution of myeloid cells and their correlation with disease progression in 40 COVID-19 patients at different stages of disease. COVID-19 patients showed a decrease in the absolute number of plasmacytoid and myeloid dendritic cells, different subset distribution of monocytes and different activation patterns of both monocytes and neutrophils, coupled to a significant reduction of HLA-DR monocyte levels. We found that some of these alterations are typical of all COVID-19 patients, while some others vary at different stages of the disease and correlate with biochemical parameters of inflammation. Collectively, these data suggest that not only the lymphoid, but also the myeloid compartment, is severely affected by SARS-CoV-2 infection.", "gemini": "SARS-CoV-2 gây ra một bệnh truyền nhiễm mới (COVID-19) mà trong đó cá nhân có thể không có triệu chứng hoặc tiến triển từ tình trạng lâm sàng nhẹ đến nặng bao gồm hội chứng suy hô hấp cấp tính và suy đa cơ quan. Các cơ chế miễn dịch có khả năng điều khiển bệnh lý trong nhiễm SARS-CoV-2 rất phức tạp và chỉ được hiểu một phần. Vẫn còn thiếu dữ liệu về các đặc điểm của các tế bào myeloid liên quan đến nhiễm virus này. Vì lý do này, chúng tôi đã nghiên cứu các cấu hình trạng thái hoạt hóa khác nhau và sự phân bố phân nhóm của các tế bào myeloid và mối tương quan của chúng với tiến triển bệnh ở 40 bệnh nhân COVID-19 ở các giai đoạn khác nhau của bệnh. Bệnh nhân COVID-19 cho thấy số lượng tế bào đuôi gai và tế bào tua dạng đuôi gai myeloid tuyệt đối giảm, sự phân bố phân nhóm khác nhau của các tế bào đơn nhân và các kiểu hoạt hóa khác nhau của cả tế bào đơn nhân và bạch cầu trung tính, cùng với sự giảm đáng kể nồng độ tế bào đơn nhân HLA-DR. Chúng tôi phát hiện ra rằng một số thay đổi này là điển hình của tất cả bệnh nhân COVID-19, trong khi một số thay đổi khác lại khác nhau ở các giai đoạn khác nhau của bệnh và tương quan với các thông số sinh hóa của tình trạng viêm. Nhìn chung, những dữ liệu này cho thấy rằng không chỉ tế bào lympho mà cả khoang myeloid cũng bị nhiễm SARS-CoV-2 ảnh hưởng nghiêm trọng."} +{"idx": 1870694, "en": "Invited for the cover of this issue is Jun Zhu, Miquel Solà and co-workers at Xiamen University, Jagiellonian University and the University of Girona. The image highlights the main roles in this study: ligands, metal centers, and adaptive aromaticity. Read the full text of the article at 10.1002/chem.202001830.", "gemini": "Được mời lên trang bìa của số báo này là Jun Zhu, Miquel Solà và các cộng sự tại Đại học Hạ Môn, Đại học Jagiellonian và Đại học Girona. Hình ảnh nêu bật vai trò chính trong nghiên cứu này: phối tử, trung tâm kim loại và tính thơm thích ứng. Đọc toàn văn bài báo tại 10.1002/chem.202001830."} +{"idx": 1870695, "en": "Invited for the cover of this issue is Lechosław Latos-Grażyński and co-workers at University of Wrocław. The image depicts two essential conformations of 31,34-dicarba[26]hexachlorins: dumbbell and rectangular with regular or doubly inverted carbocyclic rings, respectively. Read the full text of the article at 10.1002/chem.202002603.", "gemini": "Được mời lên trang bìa của số báo này là Lechosław Latos-Grażyński và các cộng sự tại Đại học Wrocław. Hình ảnh mô tả hai cấu hình thiết yếu của 31,34-dicarba[26]hexachlorin: hình quả tạ và hình chữ nhật có vòng cacbon thơm tương ứng là vòng thường hoặc vòng ngược lại gấp đôi. Đọc toàn văn bài báo tại 10.1002/chem.202002603."} +{"idx": 1870696, "en": "BACKGROUND Oxidative stress plays an important role in initiating the destruction of melanocytes, which could be one possible mechanism of vitiligo. PINK1 is an outer membrane protein of mitochondria, which protects many cells from oxidative stress through regulating mitochondrial function. However, the role of PINK1 and its effects on oxidative damage in melanocytes have not been elucidated. AIM To investigate the expression and effects of PINK1 on oxidative stress in human melanocytes. METHODS Quantitative reverse transcription-PCR and western blot analysis were used to analyse the expression of PINK1 in PIG1 melanocyte and gene downregulation models. Levels of cell viability, cell apoptosis and intracellular reactive oxygen species (ROS), mitochondrial morphology, mitochondrial membrane potential (MMP) and mitochondrial permeability transition pore (MPTP) opening were measured in PIG1 models transfected with PINK1 small interfering RNA with or without hydrogen peroxide (H2 O2 ). RESULTS We first observed the expression of PINK1 in human PIG1 melanocytes and found that downregulation of PINK1 made melanocytes more sensitive to oxidative stress induced by H2 O2 , with more cell apoptosis and increased intracellular ROS. Meanwhile, downregulation of PINK1 caused morphological changes in mitochondria, decreased the MMP and increased MPTP opening. CONCLUSIONS Our study found PINK1 plays an essential role in protecting human melanocytes from oxidative stress.", "gemini": "BỐI CẢNH Stress oxy hóa đóng vai trò quan trọng trong việc khởi phát quá trình phá hủy tế bào hắc tố, đây có thể là một cơ chế có thể xảy ra của bệnh bạch biến. PINK1 là protein màng ngoài của ty thể, có tác dụng bảo vệ nhiều tế bào khỏi stress oxy hóa thông qua việc điều hòa chức năng ty thể. Tuy nhiên, vai trò của PINK1 và tác động của nó đối với tổn thương oxy hóa ở tế bào hắc tố vẫn chưa được làm sáng tỏ. MỤC ĐÍCH Nghiên cứu biểu hiện và tác động của PINK1 đối với stress oxy hóa ở tế bào hắc tố người. PHƯƠNG PHÁP Phản ứng phiên mã ngược định lượng-PCR và phân tích vết chấm đã được sử dụng để phân tích biểu hiện của PINK1 trong tế bào hắc tố PIG1 và các mô hình hạ điều hòa gen. Nồng độ khả năng sống của tế bào, tế bào chết theo chương trình và các loài oxy phản ứng nội bào (ROS), hình thái ty thể, tiềm năng màng ty thể (MMP) và lỗ chuyển đổi màng ty thể (MPTP) được đo trong các mô hình PIG1 được chuyển gen RNA can thiệp nhỏ PINK1 có hoặc không có hydro peroxide (H2 O2 ). KẾT QUẢ Đầu tiên, chúng tôi quan sát thấy biểu hiện của PINK1 ở tế bào hắc tố PIG1 của người và thấy rằng việc hạ điều hòa PINK1 khiến tế bào hắc tố nhạy cảm hơn với stress oxy hóa do H2 O2 gây ra, với nhiều tế bào chết theo chương trình hơn và ROS nội bào tăng lên. Trong khi đó, hạ điều hòa PINK1 gây ra những thay đổi về hình thái ở ty thể, làm giảm MMP và tăng độ mở của MPTP. KẾT LUẬN Nghiên cứu của chúng tôi phát hiện ra PINK1 đóng vai trò thiết yếu trong việc bảo vệ tế bào hắc tố người khỏi stress oxy hóa."} +{"idx": 1870697, "en": "The diagnostic criteria for schizophrenia comprise a diverse range of heterogeneous symptoms. As a result, individuals each present a distinct set of symptoms despite having the same overall diagnosis. Whilst previous machine learning studies have primarily focused on dichotomous patient-control classification, we predict the severity of each individual symptom on a continuum. We applied machine learning regression within a multi-modal fusion framework to fMRI and behavioural data acquired during an auditory oddball task in 80 schizophrenia patients. Brain activity was highly predictive of some, but not all symptoms, namely hallucinations, avolition, anhedonia and attention. Critically, each of these symptoms was associated with specific functional alterations across different brain regions. We also found that modelling symptoms as an ensemble of subscales was more accurate, specific and informative than models which predict compound scores directly. In principle, this approach is transferrable to any psychiatric condition or multi-dimensional diagnosis.", "gemini": "Tiêu chuẩn chẩn đoán cho bệnh tâm thần phân liệt bao gồm một loạt các triệu chứng không đồng nhất. Do đó, mỗi cá nhân đều biểu hiện một tập hợp các triệu chứng riêng biệt mặc dù có cùng chẩn đoán chung. Trong khi các nghiên cứu học máy trước đây chủ yếu tập trung vào phân loại bệnh nhân-kiểm soát theo kiểu phân đôi, chúng tôi dự đoán mức độ nghiêm trọng của từng triệu chứng riêng lẻ trên một chuỗi liên tục. Chúng tôi đã áp dụng hồi quy học máy trong khuôn khổ hợp nhất đa phương thức vào dữ liệu fMRI và hành vi thu được trong nhiệm vụ oddball thính giác ở 80 bệnh nhân tâm thần phân liệt. Hoạt động của não rất có khả năng dự đoán một số triệu chứng, nhưng không phải tất cả các triệu chứng, cụ thể là ảo giác, mất ý chí, vô cảm và chú ý. Quan trọng là, mỗi triệu chứng này đều liên quan đến những thay đổi chức năng cụ thể ở các vùng não khác nhau. Chúng tôi cũng thấy rằng việc lập mô hình các triệu chứng như một tập hợp các thang điểm phụ chính xác hơn, cụ thể hơn và nhiều thông tin hơn so với các mô hình dự đoán trực tiếp các điểm số hợp thành. Về nguyên tắc, cách tiếp cận này có thể chuyển giao cho bất kỳ tình trạng tâm thần hoặc chẩn đoán đa chiều nào."} +{"idx": 1870698, "en": "In this research, Erzincan Tulum cheese was packaged in its original packaging material, the skin bag, small intestine, and appendix to observe the changes in its physical, chemical, and textural properties during storage day. Lactic acid% values increased in all Tulum cheeses throughout storage stage. At the end of the storage period, the highest value was determined in the sample filled in the small intestine (20.10%). All fatty acid values were increased, and the highest increase was identified in oleic acid (C 18:1) (skin bag 2.18%), linoleic acid (18:2) (appendix 0.41%), and palmitic acid (C 16:0) (small intestine 0.34%), respectively. All organic acids increased in stored sample. The highest increase among organic acids was determined to be 4.47% in lactic acid. As a result of the Texture Profile Analyses (TPA), the hardness, and adhesiveness of Tulum cheeses increased during storage periods, whereas the springiness, cohesiveness, and chewiness values decreased. Gumminess value declined in the sample filled into the appendix (with a value of 36.01), whereas it increased in the other two samples. The highest increase in hardness values was 2,520.27 N at given storage time was in the samples filled into appendix while the highest adhesive values of -49.82 were determined in the sample filled into small intestine. PRACTICAL APPLICATION: Tulum cheese is usually produced by filling sheep or goat skin bag. Goat or sheep skin bag are not always available, and the amount of cheese produced in them is excessive. Therefore, cheeses were also filled to small intestine and appendix. At the end of the study, there were no negative effects on cheeses filled with intestine and appendix. The use of small intestine and appendix has helped to develop a product that will be appreciated by the consumers with no adverse effects in the physicochemical and textural properties of cheese.", "gemini": "Trong nghiên cứu này, pho mát Erzincan Tulum được đóng gói trong vật liệu đóng gói ban đầu của nó, túi da, ruột non và ruột thừa để quan sát những thay đổi về tính chất vật lý, hóa học và kết cấu của nó trong thời gian bảo quản. Giá trị axit lactic% tăng ở tất cả các loại pho mát Tulum trong suốt thời gian bảo quản. Vào cuối thời gian bảo quản, giá trị cao nhất được xác định trong mẫu được làm đầy trong ruột non (20,10%). Tất cả các giá trị axit béo đều tăng lên, và sự gia tăng cao nhất được xác định ở axit oleic (C 18:1) (túi da 2,18%), axit linoleic (18:2) (ruột thừa 0,41%) và axit palmitic (C 16:0) (ruột non 0,34%). Tất cả các axit hữu cơ đều tăng lên trong mẫu được bảo quản. Sự gia tăng cao nhất trong số các axit hữu cơ được xác định là 4,47% ở axit lactic. Kết quả của Phân tích cấu hình kết cấu (TPA), độ cứng và độ dính của pho mát Tulum tăng lên trong thời gian bảo quản, trong khi độ đàn hồi, độ kết dính và độ dai giảm. Giá trị độ dai giảm trong mẫu được làm đầy vào ruột thừa (với giá trị là 36,01), trong khi nó tăng ở hai mẫu còn lại. Độ cứng tăng cao nhất là 2.520,27 N tại thời điểm bảo quản nhất định là ở các mẫu được làm đầy vào ruột thừa trong khi giá trị dính cao nhất là -49,82 được xác định ở mẫu được làm đầy vào ruột non. ỨNG DỤNG THỰC TẾ: Pho mát Tulum thường được sản xuất bằng cách làm đầy túi da cừu hoặc dê. Túi da dê hoặc cừu không phải lúc nào cũng có sẵn và lượng pho mát sản xuất trong túi da dê hoặc cừu là quá nhiều. Do đó, pho mát cũng được làm đầy vào ruột non và ruột thừa. Vào cuối nghiên cứu, không có tác động tiêu cực nào đối với pho mát được làm đầy bằng ruột và ruột thừa. Việc sử dụng ruột non và ruột thừa đã giúp phát triển một sản phẩm sẽ được người tiêu dùng đánh giá cao mà không có tác động bất lợi nào đến các tính chất lý hóa và kết cấu của pho mát."} +{"idx": 1870699, "en": "To make optimal use of previous experiences, important neural activity sequences must be prioritized during hippocampal replay. Integrating insights about the interplay between CA3 and CA2, we propose a conceptual framework that allows the two regions to control which sequences are reactivated. We suggest that neuromodulatory-gated plasticity and mutual inhibition enable discrete assembly sequences in both regions to support each other while suppressing competing sequences. This perspective provides a coherent interpretation for a variety of seemingly disconnected functional properties of CA2 and paves the way for a more general understanding of CA2.", "gemini": "Để sử dụng tối ưu các kinh nghiệm trước đó, các chuỗi hoạt động thần kinh quan trọng phải được ưu tiên trong quá trình phát lại hồi hải mã. Tích hợp những hiểu biết về sự tương tác giữa CA3 và CA2, chúng tôi đề xuất một khuôn khổ khái niệm cho phép hai vùng kiểm soát các chuỗi nào được kích hoạt lại. Chúng tôi cho rằng tính dẻo được kiểm soát bởi điều biến thần kinh và sự ức chế lẫn nhau cho phép các chuỗi lắp ráp riêng biệt ở cả hai vùng hỗ trợ lẫn nhau trong khi ức chế các chuỗi cạnh tranh. Quan điểm này cung cấp một cách giải thích mạch lạc cho nhiều tính chất chức năng có vẻ không liên quan của CA2 và mở đường cho sự hiểu biết chung hơn về CA2."} +{"idx": 1870700, "en": "Selective bond formations are one of the most important reactions in organic synthesis. In the Lewis acid mediated electrophile reactions of carbonyls, the selective formation of a carbonyl-acid complex plays a critical role in determining selectivity, which is based on the difference in the coordinative interaction between the carbonyl and Lewis acid center. Although this strategy has attained progress in selective bond formations, the discrimination between similarly sized aromatic and aliphatic carbonyls that have no functional anchors to strongly interact with the metal center still remains a challenging issue. Herein, this work focuses on molecular recognition driven by dispersion interactions within some aromatic moieties. A Lewis acid catalyst with a π-space cavity, which is referred to as a π-pocket, as the recognition site for aromatic carbonyls is designed. Cage-shaped borates 1B with various π-pockets demonstrated significant chemoselectivity for aromatic aldehydes 3 b-f over that of aliphatic 3 a in competitive hetero-Diels-Alder reactions. The effectiveness of our catalysts was also evidenced by intramolecular recognition of the aromatic carbonyl within a dicarbonyl substrate. Mechanistic and theoretical studies demonstrated that the selective activation of aromatic substrates was driven by the preorganization step with a larger dispersion interaction, rather than the rate-determining step of the C-C bond formation, and this was likely to contribute to the preferred activation of aromatic substrates over that of aliphatic ones.", "gemini": "Sự hình thành liên kết chọn lọc là một trong những phản ứng quan trọng nhất trong tổng hợp hữu cơ. Trong các phản ứng ái điện của cacbonyl có sự xúc tác của axit Lewis, sự hình thành phức hợp axit cacbonyl một cách chọn lọc đóng vai trò quan trọng trong việc xác định độ chọn lọc, dựa trên sự khác biệt về tương tác phối hợp giữa cacbonyl và trung tâm axit Lewis. Mặc dù chiến lược này đã đạt được tiến bộ trong việc hình thành liên kết chọn lọc, nhưng sự phân biệt giữa các cacbonyl thơm và aliphatic có kích thước tương tự, không có neo chức năng để tương tác mạnh với trung tâm kim loại vẫn là một vấn đề đầy thách thức. Ở đây, công trình này tập trung vào sự nhận biết phân tử được thúc đẩy bởi các tương tác phân tán trong một số nhóm thơm. Một chất xúc tác axit Lewis có khoang không gian π, được gọi là túi π, là vị trí nhận biết đối với các cacbonyl thơm được thiết kế. Các borat dạng lồng 1B với nhiều túi π khác nhau đã chứng minh được độ chọn lọc hóa học đáng kể đối với các aldehyd thơm 3 b-f so với 3 a aliphatic trong các phản ứng hetero-Diels-Alder cạnh tranh. Hiệu quả của chất xúc tác của chúng tôi cũng được chứng minh bằng sự nhận biết phân tử nội bộ của cacbonyl thơm trong chất nền dicacbonyl. Các nghiên cứu cơ học và lý thuyết đã chứng minh rằng sự hoạt hóa chọn lọc các chất nền thơm được thúc đẩy bởi bước sắp xếp trước với tương tác phân tán lớn hơn, chứ không phải bước xác định tốc độ của sự hình thành liên kết CC, và điều này có khả năng góp phần vào sự hoạt hóa ưa thích của các chất nền thơm so với các chất nền aliphatic."} +{"idx": 1870701, "en": "Human activity is known to leave significant effects on indoor airflow patterns. These patterns are carefully designed for many facilities such as cleanrooms, pharmaceutical settings, and healthcare environments, where human-induced wakes contribute to the transport of contaminants. Therefore, the knowledge about these wakes as it relates to indoor air quality is critical. As a result, a series of experiments were conducted in a controlled chamber to study the three-dimensional effects of true human walking on airflow. Experiments were designed to capture the effect of human walking under three different flow conditions, and for two different walking schemes. The results show that the effect of walking on the airflow is not negligible and can sustain up to 10 seconds after the moving body has passed. Walking on a straight line creates significant change in the velocity normal to the walking path and vertical to the plane of walking movement. These changes were detectable till 1.0 m away from the walking track. Also, the similarity between airflow patterns of walking once and twice illustrated a promising opportunity of predicting the flow patterns of random walk from a set of base cases.", "gemini": "Hoạt động của con người được biết là để lại những tác động đáng kể đến các mô hình luồng không khí trong nhà. Các mô hình này được thiết kế cẩn thận cho nhiều cơ sở như phòng sạch, cơ sở dược phẩm và môi trường chăm sóc sức khỏe, nơi các luồng gió do con người tạo ra góp phần vận chuyển các chất gây ô nhiễm. Do đó, kiến thức về những luồng gió này liên quan đến chất lượng không khí trong nhà là rất quan trọng. Kết quả là, một loạt các thí nghiệm đã được tiến hành trong một buồng kiểm soát để nghiên cứu các hiệu ứng ba chiều của việc đi bộ thực sự của con người đối với luồng không khí. Các thí nghiệm được thiết kế để nắm bắt hiệu ứng đi bộ của con người trong ba điều kiện luồng khác nhau và đối với hai kiểu đi bộ khác nhau. Kết quả cho thấy rằng tác động của việc đi bộ đối với luồng không khí không phải là không đáng kể và có thể duy trì tới 10 giây sau khi cơ thể di chuyển đã đi qua. Đi bộ theo đường thẳng tạo ra sự thay đổi đáng kể về vận tốc vuông góc với đường đi và vuông góc với mặt phẳng chuyển động đi bộ. Những thay đổi này có thể phát hiện được cho đến 1,0 m tính từ đường đi bộ. Ngoài ra, sự giống nhau giữa các mô hình luồng không khí khi đi bộ một lần và hai lần minh họa cho một cơ hội đầy hứa hẹn để dự đoán các mô hình luồng của chuyển động ngẫu nhiên từ một tập hợp các trường hợp cơ sở."} +{"idx": 1870702, "en": "INTRODUCTION There are limited data on the impact of haemophilia on health status and health-related quality of life (HRQL) in people with non-severe (mild and moderate) haemophilia. AIM To evaluate the health status of people living with mild or moderate haemophilia. METHODS Data on respondents with no bleeding disorder (NoBD), mild and moderate haemophilia patients were drawn from the PROBE study. Respondents were enrolled using network patient organizations. This analysis was performed as a cross-sectional study. Primary outcomes were reported bleeding, acute and chronic pain, activities of daily living and HRQL. RESULTS A total of 862 respondents with NoBD (n = 173), mild (n = 102) and moderate (n = 134) haemophilia were eligible, with a median age of 33, 42 and 43, respectively. In relation to haemophilia-related sequalae, 53% of male and 29% of female patients with mild and 83% of males with moderate haemophilia had more than 2-3 bleeds in the last 12 months. Reporting of acute and chronic pain is less in those with NoBD compared to the mild and moderate cohorts for both genders. Multivariate analysis demonstrates significant reductions in quality of life using VAS, EQ-5D-5L and PROBE for males with mild and moderate haemophilia (P ≤ .001) with only PROBE indicating a significant reduction for females with mild (P = .002). CONCLUSION People affected by mild or moderate haemophilia report a significant HRQL impact due to haemophilia-related bleeding. Future research is needed to identify the optimal care management of patients with mild and moderate haemophilia.", "gemini": "GIỚI THIỆU Có dữ liệu hạn chế về tác động của bệnh máu khó đông đến tình trạng sức khỏe và chất lượng cuộc sống liên quan đến sức khỏe (HRQL) ở những người bị bệnh máu khó đông không nghiêm trọng (nhẹ và trung bình). MỤC ĐÍCH Đánh giá tình trạng sức khỏe của những người mắc bệnh máu khó đông nhẹ hoặc trung bình. PHƯƠNG PHÁP Dữ liệu về những người trả lời không bị rối loạn chảy máu (NoBD), bệnh nhân bị máu khó đông nhẹ và trung bình được lấy từ nghiên cứu PROBE. Những người trả lời được tuyển dụng thông qua các tổ chức bệnh nhân mạng lưới. Phân tích này được thực hiện như một nghiên cứu cắt ngang. Kết quả chính được báo cáo là chảy máu, đau cấp tính và mãn tính, các hoạt động trong cuộc sống hàng ngày và HRQL. KẾT QUẢ Tổng cộng 862 người trả lời không mắc bệnh NoBD (n = 173), máu khó đông nhẹ (n = 102) và trung bình (n = 134) đủ điều kiện, với độ tuổi trung bình lần lượt là 33, 42 và 43. Liên quan đến di chứng liên quan đến bệnh máu khó đông, 53% nam giới và 29% nữ giới bị máu khó đông nhẹ và 83% nam giới bị máu khó đông trung bình bị chảy máu nhiều hơn 2-3 lần trong 12 tháng qua. Việc báo cáo đau cấp tính và mãn tính ít hơn ở những người không mắc bệnh NoBD so với nhóm máu khó đông nhẹ và trung bình ở cả hai giới. Phân tích đa biến chứng minh rằng chất lượng cuộc sống giảm đáng kể khi sử dụng VAS, EQ-5D-5L và PROBE cho nam giới bị máu khó đông nhẹ và trung bình (P ≤ 0,001) với chỉ PROBE cho thấy sự giảm đáng kể ở nữ giới bị máu khó đông nhẹ (P = 0,002). KẾT LUẬN Những người bị máu khó đông nhẹ hoặc trung bình báo cáo rằng HRQL bị ảnh hưởng đáng kể do chảy máu liên quan đến bệnh máu khó đông. Cần nghiên cứu thêm để xác định cách quản lý chăm sóc tối ưu cho bệnh nhân bị máu khó đông nhẹ và trung bình."} +{"idx": 1870703, "en": "Developing highly-efficient non-noble-metal electrocatalysts for water splitting is crucial for the development of clean and reversible hydrogen energy. Introducing lattice strain is an effective strategy to develop efficient electrocatalysts. However, lattice strain is typically co-created with heterostructure, vacancy, or substrate effects, which complicate the identification of the strain-activity correlation. Herein, a series of lattice-strained homogeneous NiSx Se1- x nanosheets@nanorods hybrids are designed and synthesized by a facile strategy. The NiS0.5 Se0.5 with ≈2.7% lattice strain exhibits outstanding activity for hydrogen and oxygen evolution reaction (HER/OER), affording low overpotentials of 70 and 257 mV at 10 mA cm-2 , respectively, as well as excellent long-term durability even at a large current density of 100 mA cm-2 (300 h), significantly superior to other benchmarks and the precious metal catalysts. Experimental and theoretical calculation results reveal that the generated lattice strain decreases the metal d-orbital overlap, leading to a narrower bandwidth and a closer d-band center toward the Fermi level. Thus, NiS0.5 Se0.5 possesses favorable H* adsorption kinetics for HER and lower energy barriers for OER. This work provides a new insight to regulate the lattice strain of advanced catalyst materials and further improve the performance of energy conversion technologies.", "gemini": "Phát triển các chất xúc tác điện không phải kim loại quý có hiệu suất cao để phân tách nước là rất quan trọng đối với sự phát triển của năng lượng hydro sạch và có thể đảo ngược. Việc đưa vào ứng suất mạng là một chiến lược hiệu quả để phát triển các chất xúc tác điện hiệu quả. Tuy nhiên, ứng suất mạng thường được tạo ra cùng với hiệu ứng dị cấu trúc, chỗ trống hoặc chất nền, làm phức tạp việc xác định mối tương quan ứng suất-hoạt động. Ở đây, một loạt các tấm nano NiSx Se1-x đồng nhất có ứng suất mạng @ nanorod lai được thiết kế và tổng hợp bằng một chiến lược dễ dàng. NiS0,5 Se0,5 với ứng suất mạng ≈2,7% thể hiện hoạt động vượt trội đối với phản ứng giải phóng hydro và oxy (HER / OER), tạo ra điện thế quá thấp là 70 và 257 mV ở 10 mA cm-2, cũng như độ bền lâu dài tuyệt vời ngay cả ở mật độ dòng điện lớn 100 mA cm-2 (300 giờ), vượt trội đáng kể so với các chuẩn mực khác và các chất xúc tác kim loại quý. Kết quả tính toán thực nghiệm và lý thuyết cho thấy ứng suất mạng tạo ra làm giảm sự chồng chéo của quỹ đạo d kim loại, dẫn đến băng thông hẹp hơn và tâm d gần hơn về phía mức Fermi. Do đó, NiS0,5 Se0,5 sở hữu động học hấp phụ H * thuận lợi cho HER và rào cản năng lượng thấp hơn cho OER. Công trình này cung cấp một hiểu biết mới để điều chỉnh ứng suất mạng của vật liệu xúc tác tiên tiến và cải thiện hơn nữa hiệu suất của các công nghệ chuyển đổi năng lượng."} +{"idx": 1870704, "en": "Accumulation and aggregation of the intrinsically disordered protein α-synuclein (α-Syn) into amyloid fibrils are hallmarks of a series of heterogeneous neurodegenerative disorders, known as synucleinopathies and most notably Parkinson's disease (PD). The crucial role of α-Syn aggregation in PD makes it an attractive target for the development of disease-modifying therapeutics that would inhibit α-Syn aggregation or disrupt its preformed fibrillar assemblies. To this end, we have designed and synthesized two naphthoquinone-dopamine-based hybrid small molecules, NQDA and Cl-NQDA, and demonstrated their ability to inhibit in vitro amyloid formation by α-Syn using ThT assay, CD, TEM, and Congo red birefringence. Moreover, these hybrid molecules efficiently disassembled preformed fibrils of α-Syn into nontoxic species, as evident from LUV leakage assay. NQDA and Cl-NQDA were found to have low cytotoxicity and they attenuated the toxicity induced by α-Syn towards SH-SY5Y neuroblastoma cells. NQDA was found to efficiently cross an in vitro human blood-brain barrier model. These naphthoquinone-dopamine based derivatives can be an attractive scaffold for therapeutic design towards PD.", "gemini": "Sự tích tụ và kết tập của protein α-synuclein (α-Syn) vốn không có trật tự thành các sợi fibril amyloid là đặc điểm của một loạt các rối loạn thoái hóa thần kinh không đồng nhất, được gọi là bệnh synucleinopathy và đáng chú ý nhất là bệnh Parkinson (PD). Vai trò quan trọng của sự kết tập α-Syn trong PD khiến nó trở thành mục tiêu hấp dẫn cho sự phát triển của các phương pháp điều trị làm thay đổi bệnh, có thể ức chế sự kết tập α-Syn hoặc phá vỡ các tập hợp fibril đã hình thành trước của nó. Để đạt được mục đích này, chúng tôi đã thiết kế và tổng hợp hai phân tử nhỏ lai dựa trên naphthoquinone-dopamine, NQDA và Cl-NQDA, và chứng minh khả năng ức chế sự hình thành amyloid trong ống nghiệm của α-Syn bằng xét nghiệm ThT, CD, TEM và lưỡng chiết đỏ Congo. Hơn nữa, các phân tử lai này giải tán hiệu quả các sợi fibril α-Syn đã hình thành trước thành các loài không độc hại, như bằng chứng từ xét nghiệm rò rỉ LUV. NQDA và Cl-NQDA được phát hiện có độc tính tế bào thấp và chúng làm giảm độc tính do α-Syn gây ra đối với các tế bào nguyên bào thần kinh đệm SH-SY5Y. NQDA được phát hiện có khả năng vượt qua hiệu quả mô hình hàng rào máu não của người trong ống nghiệm. Các dẫn xuất dựa trên naphthoquinone-dopamine này có thể là một khung hấp dẫn cho thiết kế trị liệu hướng đến PD."} +{"idx": 1870705, "en": "BACKGROUND A biological concentrate was produced from cultures of an Avène aquatic microflora isolate, namely Aquaphilus dolomiae. Some of the beneficial effects on diseased and damaged skin are thought to be due to the presence of this microorganism. AIMS An extract of A. dolomiae (A. dolomiae extract-G2, ADE-G2) was evaluated for its wound-healing effects using in vitro and ex vivo models of injured skin. METHODS The effect of ADE-G2 on the proliferation of fibroblasts, migration of keratinocytes and re-epithelialization of ex vivo wounded skin explants was measured. Antimicrobial protection by ADE-G2 was measured by analysing the gene expression of a panel of antimicrobial proteins (AMPs) in keratinocytes (RNASE7, S100A7, DEFB4A/B and DEFb103B), as well as the protein encoded by DEFB4A-B (hBD2) in the medium. RESULTS ADE-G2 increased fibroblast proliferation and keratinocyte migration, as well as re-epithelialization of wounded ex vivo skin. ADE-G2 induced the expression of all AMP genes analysed in keratinocytes, as well as stimulated the release in to the medium of hBD2 peptide, encoded by DEFB4A/B. CONCLUSIONS We have shown the broad spectrum of the repairing properties of the A. dolomiae extract, ADE-G2. These results support the use of ADE-G2 as a promising component for use in formulations aimed at repairing skin, limiting wound superinfection and preventing complicated wounds.", "gemini": "BỐI CẢNH Một hợp chất sinh học cô đặc được sản xuất từ các nền nuôi cấy của một chủng vi khuẩn thủy sinh Avène, cụ thể là Aquaphilus dolomiae. Một số tác dụng có lợi đối với làn da bị bệnh và bị tổn thương được cho là do sự hiện diện của vi sinh vật này. MỤC ĐÍCH Một chiết xuất của A. dolomiae (chiết xuất A. dolomiae-G2, ADE-G2) đã được đánh giá về tác dụng chữa lành vết thương của nó bằng cách sử dụng các mô hình trong ống nghiệm và trên cơ thể sống của da bị thương. PHƯƠNG PHÁP Tác dụng của ADE-G2 đối với sự tăng sinh của nguyên bào sợi, sự di chuyển của tế bào sừng và sự tái biểu mô của các mẫu da bị thương trên cơ thể sống đã được đo lường. Khả năng bảo vệ chống lại vi khuẩn của ADE-G2 được đo bằng cách phân tích biểu hiện gen của một nhóm các protein kháng khuẩn (AMP) trong tế bào sừng (RNASE7, S100A7, DEFB4A/B và DEFb103B), cũng như protein được mã hóa bởi DEFB4A-B (hBD2) trong môi trường. KẾT QUẢ ADE-G2 làm tăng sự tăng sinh của nguyên bào sợi và sự di chuyển của tế bào sừng, cũng như sự tái biểu mô của da bị thương trên cơ thể sống. ADE-G2 gây ra sự biểu hiện của tất cả các gen AMP được phân tích trong tế bào sừng, cũng như kích thích giải phóng vào môi trường của peptide hBD2, được mã hóa bởi DEFB4A/B. KẾT LUẬN Chúng tôi đã chỉ ra phổ rộng các đặc tính phục hồi của chiết xuất A. dolomiae, ADE-G2. Những kết quả này hỗ trợ việc sử dụng ADE-G2 như một thành phần đầy hứa hẹn để sử dụng trong các công thức nhằm phục hồi da, hạn chế nhiễm trùng thứ phát ở vết thương và ngăn ngừa các vết thương phức tạp."} +{"idx": 1870706, "en": "BACKGROUND Inflammatory skin disorders, including atopic dermatitis (AD), associated pruritus and sensitive skin, have a complex multifactorial pathogenesis including neurogenic inflammation involving the release in blood and skin of neurotransmitters such as substance P (SP). AIMS AND METHODS In vitro models evaluated the effect of the original biological extract of Aquaphilus dolomiae extract-G3 (ADE-G3) on cutaneous neurogenic inflammation. RESULTS ADE-G3 significantly inhibited SP-stimulated release of IL-1β and TNF-α from normal human epidermal keratinocytes; significantly and dose-dependently inhibited SP-stimulated activation of human mast cells; significantly inhibited veratridine-stimulated release of SP from human sensory neurons; modulated expression of genes involved in lipid synthesis, innate immunity, corneocyte scaffolding and epidermal differentiation in a histamine-sensitized reconstructed human epidermis model; and, when applied topically to ex vivo human explants, inhibited IL-8 and histamine release. CONCLUSIONS Topically applied ADE-G3, once formulated, may improve neuro-inflammation in patients with inflammatory skin disorders.", "gemini": "BỐI CẢNH Các rối loạn da viêm, bao gồm viêm da dị ứng (AD), ngứa liên quan và da nhạy cảm, có cơ chế bệnh sinh phức tạp do nhiều yếu tố bao gồm cả tình trạng viêm thần kinh liên quan đến việc giải phóng chất dẫn truyền thần kinh như chất P (SP) trong máu và da. MỤC ĐÍCH VÀ PHƯƠNG PHÁP Các mô hình trong ống nghiệm đã đánh giá tác dụng của chiết xuất sinh học ban đầu của chiết xuất Aquaphilus dolomiae-G3 (ADE-G3) đối với tình trạng viêm thần kinh ở da. KẾT QUẢ ADE-G3 ức chế đáng kể sự giải phóng IL-1β và TNF-α do SP kích thích từ các tế bào sừng biểu bì ở người bình thường; ức chế đáng kể và phụ thuộc vào liều lượng sự hoạt hóa do SP kích thích của các tế bào mast ở người; ức chế đáng kể sự giải phóng SP do veratridine kích thích từ các tế bào thần kinh cảm giác ở người; điều chỉnh biểu hiện của các gen liên quan đến quá trình tổng hợp lipid, miễn dịch bẩm sinh, cấu trúc tế bào sừng và biệt hóa biểu bì trong mô hình biểu bì người tái tạo nhạy cảm với histamine; và khi được bôi tại chỗ lên các mẫu da trên cơ thể sống của người, nó ức chế sự giải phóng IL-8 và histamine. KẾT LUẬN ADE-G3 bôi tại chỗ, sau khi được bào chế, có thể cải thiện tình trạng viêm thần kinh ở những bệnh nhân mắc các rối loạn da viêm."} +{"idx": 1870707, "en": "BACKGROUND Thermal Spring Water (TSW) has been recognized to have beneficial effects on skin; however, the mechanisms underlying these are not completely elucidated. AIMS We compared the effects of Avène TSW with mineral-rich (MR) TSW on the biomechanical properties of the skin using mechanistic ex vivo assays and clinical studies. METHODS Ex vivo studies included the effect of both TSWs on the structure of the surface of human skin explants using scanning electron microscopy (SEM); mineral elemental content on the skin surface using SEM coupled to energy dispersing X-ray spectroscopy; and the stress properties of the stratum corneum (SC) when exposed to dehydration. Human clinical studies were conducted to compare the soothing effect of TSWs after a dermatological chemical peeling of face skin and to evaluate the overall sensitive scale of consumers using Avène TSW for 7 days. RESULTS Both TSWs preserved surface skin ultrastructure; however, crystals formed from MR-TSW were needle-like and formed small grains, present in clusters heterogeneously spread over the surface. Needle crystals were mainly composed of calcium, while small clusters were mainly composed of sulphur. By contrast, Avène TSW-formed crystals composed of sodium and chlorine only were regular in shape and homogeneously distributed across the skin surface. Peak stress of SC layers was increased by MR-TSW, whereas Avène TSW showed a comparatively reduced effect on dehydration and stress. The difference in the two TSW types was reflected in clinical findings comparing postpeeling redness after TSW application. Avène TSW significantly decreased postpeeling redness, while MR-TSW increased it. The overall sensitive scale of consumers was decreased by 47% using Avène TSW for 7 days. CONCLUSIONS Avène TSW decreases postpeeling redness and soothes sensitive skin in human volunteers. Mechanistic studies suggested that differences in biomechanical effects could be linked to differences in calcium content of the TSW.", "gemini": "BỐI CẢNH Nước suối khoáng nóng (TSW) được công nhận là có tác dụng có lợi cho da; tuy nhiên, các cơ chế cơ bản đằng sau những tác dụng này vẫn chưa được làm sáng tỏ hoàn toàn. MỤC ĐÍCH Chúng tôi đã so sánh tác động của Avène TSW với TSW giàu khoáng chất (MR) đối với các đặc tính cơ học sinh học của da bằng cách sử dụng các xét nghiệm trên cơ thể sống theo cơ chế và các nghiên cứu lâm sàng. PHƯƠNG PHÁP Các nghiên cứu trên cơ thể sống bao gồm tác động của cả hai loại TSW đối với cấu trúc bề mặt của các mẫu da người bằng cách sử dụng kính hiển vi điện tử quét (SEM); hàm lượng nguyên tố khoáng trên bề mặt da bằng SEM kết hợp với phổ kế tia X phân tán năng lượng; và các đặc tính ứng suất của lớp sừng (SC) khi tiếp xúc với tình trạng mất nước. Các nghiên cứu lâm sàng trên người đã được tiến hành để so sánh tác dụng làm dịu của TSW sau khi lột da hóa học của da mặt và để đánh giá thang điểm nhạy cảm tổng thể của người tiêu dùng sử dụng Avène TSW trong 7 ngày. KẾT QUẢ Cả hai loại TSW đều bảo tồn cấu trúc siêu vi của bề mặt da; tuy nhiên, các tinh thể hình thành từ MR-TSW giống như kim và tạo thành các hạt nhỏ, có trong các cụm phân bố không đồng đều trên bề mặt. Các tinh thể hình kim chủ yếu bao gồm canxi, trong khi các cụm nhỏ chủ yếu bao gồm lưu huỳnh. Ngược lại, các tinh thể hình thành từ Avène TSW chỉ bao gồm natri và clo có hình dạng đều và phân bố đồng đều trên bề mặt da. Độ căng đỉnh của các lớp SC tăng lên do MR-TSW, trong khi Avène TSW cho thấy tác dụng giảm đáng kể đối với tình trạng mất nước và ứng suất. Sự khác biệt giữa hai loại TSW được phản ánh trong các phát hiện lâm sàng so sánh tình trạng đỏ sau khi lột da sau khi sử dụng TSW. Avène TSW làm giảm đáng kể tình trạng đỏ sau khi lột da, trong khi MR-TSW làm tăng tình trạng này. Thang điểm nhạy cảm tổng thể của người tiêu dùng đã giảm 47% khi sử dụng Avène TSW trong 7 ngày. KẾT LUẬN Avène TSW làm giảm tình trạng đỏ sau khi lột da và làm dịu làn da nhạy cảm ở những người tình nguyện. Các nghiên cứu theo cơ chế cho thấy rằng sự khác biệt về tác dụng cơ học sinh học có thể liên quan đến sự khác biệt về hàm lượng canxi của TSW."} +{"idx": 1870708, "en": "The beneficial effects of Avène Thermal Spring Water (TSW), a low mineral content spring water, on chronic skin diseases have been recognized for more than two centuries. This article provides a brief overview of efficacy and tolerance data for Avène TSW from clinical studies conducted at the Avène Hydrotherapy Center in patients with chronic inflammatory skin diseases or temporary skin injuries. Avène TSW hydrotherapy is effective as adjuvant management for chronic skin diseases and dermatological conditions, relieving subjective and physical symptoms with excellent tolerance.", "gemini": "Các tác dụng có lợi của Nước suối khoáng Avène (TSW) - một loại nước suối có hàm lượng khoáng chất thấp, đối với các bệnh về da mãn tính đã được công nhận trong hơn hai thế kỷ. Bài viết này cung cấp một bản tóm tắt ngắn gọn về dữ liệu về hiệu quả và dung nạp của Avène TSW từ các nghiên cứu lâm sàng được thực hiện tại Trung tâm Thủy trị Avène ở những bệnh nhân mắc các bệnh về da viêm mãn tính hoặc các thương tích tạm thời trên da. Thủy trị Avène TSW có hiệu quả như một phương pháp điều trị hỗ trợ cho các bệnh về da mãn tính và các tình trạng da liễu, làm giảm các triệu chứng chủ quan và thể chất với khả năng dung nạp tuyệt vời."} +{"idx": 1870709, "en": "Aquaphilus dolomiae (AD) is a unique isolate from Avène Thermal Spring Water. I-Modulia, the first biotech extract from culture of AD, was used as immune modulator in Th2 inflammatory models. In this short publication, firstly we describe generation of two AD de novo extracts specifically designed for repairing and for neuroinflammation modulation activities which will be described, respectively, in two other articles in this supplement. Finally, for I-modulia, we describe new data on inhibition of human mast cell degranulation in vitro and its effect on substance P-induced neurogenic inflammation on ex vivo human skin explants.", "gemini": "Aquaphilus dolomiae (AD) là một loại phân lập độc đáo từ Nước suối khoáng Avène. I-Modulia, chiết xuất công nghệ sinh học đầu tiên từ nuôi cấy AD, được sử dụng làm chất điều hòa miễn dịch trong các mô hình viêm Th2. Trong ấn phẩm ngắn này, trước tiên chúng tôi mô tả việc tạo ra hai chiết xuất AD de novo được thiết kế đặc biệt để sửa chữa và cho các hoạt động điều chế thần kinh sẽ được mô tả tương ứng trong hai bài báo khác trong phần bổ sung này. Cuối cùng, đối với I-modulia, chúng tôi mô tả dữ liệu mới về việc ức chế quá trình giải hạt tế bào mast ở người trong ống nghiệm và tác dụng của nó đối với chứng viêm thần kinh do chất P gây ra trên các mẫu da người ex vivo."} +{"idx": 1870710, "en": "Avène Thermal Spring Water (Avène TSW) has been used since 1743 in the treatment of various pathologies. The hydrothermal system is delimited by a reservoir mainly composed by dolomite and quartz in which meteoric waters infiltrate allowing rock-water interactions that control the water's chemical composition. Water analysis and geochemical calculations highlight that water composition is stable and low mineral salt content are controlled by natural geochemical equilibrium and the solubility of the minerals calcite, dolomite and quartz according to temperature and CO2 pressure within the reservoir. In addition, the annual monitoring of surface waters sampled on the impluvium surface area confirms the absence of anthropic impact on water quality. The forest and the low human activity of the watershed contribute to preserve the natural groundwater quality. This confirms that nowadays, the recharge water is still of great quality for the future generation.", "gemini": "Nước suối khoáng Avène (Avène TSW) đã được sử dụng từ năm 1743 trong điều trị nhiều bệnh lý khác nhau. Hệ thống thủy nhiệt được phân định bởi một hồ chứa chủ yếu bao gồm dolomit và thạch anh, trong đó nước mưa thấm vào cho phép tương tác giữa nước và đá kiểm soát thành phần hóa học của nước. Phân tích nước và tính toán địa hóa làm nổi bật rằng thành phần nước ổn định và hàm lượng muối khoáng thấp được kiểm soát bởi cân bằng địa hóa tự nhiên và độ hòa tan của các khoáng chất canxit, dolomit và thạch anh theo nhiệt độ và áp suất CO2 trong hồ chứa. Ngoài ra, việc theo dõi hàng năm đối với các nguồn nước mặt được lấy mẫu trên diện tích bề mặt của lưu vực ngầm xác nhận không có tác động của con người đối với chất lượng nước. Rừng và hoạt động của con người thấp trong lưu vực góp phần bảo vệ chất lượng nước ngầm tự nhiên. Điều này khẳng định rằng hiện nay, nước ngầm vẫn có chất lượng tuyệt vời cho các thế hệ tương lai."} +{"idx": 1870711, "en": "BACKGROUND Avène Thermal Spring Water (TSW) exhibits therapeutic properties in the treatment of skin pathologies. Arising from a dolomitic aquifer system, its physico-chemical properties are well-established and its bacteriological quality regularly monitored. The microbiota of this aquifer have been characterized. OBJECTIVES We aimed to describe the structure of the bacterial community inhabiting the deep aquifer and to examine its dynamics over time. METHODS The Avène TSW was collected at the catchment point and filtered through 0.1 µm pore size filters. The sampling was carried out every 3 months to generate a 4-year time series. The DNA extracted from filters was analysed using high-throughput 16S rRNA gene amplicon sequencing, and the microorganisms and their contribution were characterized by the taxonomic assignment of sequence variants generated from each sample. RESULTS Bacteria were distributed into 39 phyla. Nitrospirae and Proteobacteria were the most prevalent, accounting for 38% and 23% of the total community on average, respectively. A stable pattern was observed throughout the study. A few bacterial species were always detected, forming a core community of likely chemolithoautotrophic organisms which might use energy sources and nutrients produced from water-bedrock interactions. Most of the species were distantly related to organisms described to date. CONCLUSIONS Avène TSW provided by the deep aquifer system harbours a unique microbial community, shaped by the physico-chemical characteristics of the deep environment. Its remarkable stability over time has revealed a high level of confinement of the water resource.", "gemini": "HOÀN CẢNH Nước suối khoáng Avène (TSW) thể hiện các đặc tính điều trị trong việc điều trị các bệnh lý về da. Xuất phát từ hệ thống tầng chứa nước dolomit, các đặc tính vật lý - hóa học của nước được thiết lập rõ ràng và chất lượng vi khuẩn của nước được theo dõi thường xuyên. Hệ vi sinh vật của tầng chứa nước này đã được mô tả. MỤC TIÊU Chúng tôi hướng đến việc mô tả cấu trúc của cộng đồng vi khuẩn cư trú trong tầng chứa nước sâu và kiểm tra động lực của nó theo thời gian. PHƯƠNG PHÁP Nước suối khoáng Avène TSW được thu thập tại điểm thu nước và lọc qua các bộ lọc có kích thước lỗ lọc 0,1 µm. Việc lấy mẫu được thực hiện 3 tháng một lần để tạo ra một chuỗi thời gian trong 4 năm. DNA được chiết xuất từ các bộ lọc đã được phân tích bằng giải trình tự đoạn gen 16S rRNA amplicon thông lượng cao và các vi sinh vật cùng sự đóng góp của chúng được đặc trưng bởi sự phân loại theo loài của các biến thể trình tự tạo ra từ mỗi mẫu. KẾT QUẢ Vi khuẩn được phân bố thành 39 ngành. Nitrospirae và Proteobacteria là những loài phổ biến nhất, chiếm lần lượt trung bình 38% và 23% tổng số quần thể. Một mô hình ổn định đã được quan sát trong suốt quá trình nghiên cứu. Một số loài vi khuẩn luôn được phát hiện, tạo thành một cộng đồng cốt lõi của các sinh vật có khả năng tự dưỡng hóa học có thể sử dụng các nguồn năng lượng và chất dinh dưỡng được tạo ra từ các tương tác giữa nước và đá nền. Hầu hết các loài có quan hệ họ hàng xa với các sinh vật được mô tả cho đến nay. KẾT LUẬN Nước suối khoáng Avène TSW được cung cấp bởi hệ thống tầng chứa nước sâu chứa một cộng đồng vi sinh vật độc đáo, được hình thành bởi các đặc tính vật lý - hóa học của môi trường sâu. Sự ổn định đáng chú ý của nó theo thời gian đã cho thấy mức độ giới hạn cao của nguồn nước."} +{"idx": 1870712, "en": "The hydrotherapy centre in Avène, France, is used extensively to treat inflammatory skin diseases. Nevertheless, the immune mechanisms targeted by Avène Thermal Spring Water (TSW) are not fully understood. Here, we review the main results reported regarding the effects of Avène TSW on the immune system. In particular, mast cells, dendritic cells (DCs) and CD4+ T cells have been shown to be modulated by Avène TSW. All in all, the studies carried out on the effects of Avène TSW on leucocytes indicate that this water is endowed with a tolerogenic potential.", "gemini": "Trung tâm thủy liệu pháp ở Avène, Pháp, được sử dụng rộng rãi để điều trị các bệnh về da có tính chất viêm. Tuy nhiên, các cơ chế miễn dịch mà Nước suối khoáng Avène (TSW) nhắm đến vẫn chưa được hiểu đầy đủ. Ở đây, chúng tôi xem xét các kết quả chính được báo cáo liên quan đến tác dụng của Nước suối khoáng Avène TSW đối với hệ thống miễn dịch. Đặc biệt, các tế bào mast, tế bào dạng gai (DC) và tế bào T CD4+ đã được chứng minh là bị điều chỉnh bởi Nước suối khoáng Avène TSW. Nhìn chung, các nghiên cứu được thực hiện về tác dụng của Nước suối khoáng Avène TSW đối với bạch cầu chỉ ra rằng loại nước này có tiềm năng gây dung nạp miễn dịch."} +{"idx": 1870713, "en": "Originally developed to improve the management of serious chronic diseases such as diabetes mellitus, obesity, cardiovascular diseases, asthma and as aftercare following oncological treatments, therapeutic patient education (TPE) has been extended to other situations, including chronic dermatoses such as atopic dermatitis, eczema and psoriasis. In this article, we provide a brief overview of the development, implementation and evaluation of TPE at the Avène-Les-Bains Hydrotherapy Center, France.", "gemini": "Ban đầu được phát triển để cải thiện việc quản lý các bệnh mãn tính nghiêm trọng như đái tháo đường, béo phì, bệnh tim mạch, hen suyễn và như chăm sóc sau khi điều trị ung thư, giáo dục bệnh nhân điều trị (TPE) đã được mở rộng sang các tình huống khác, bao gồm cả bệnh da mãn tính như viêm da dị ứng, chàm và bệnh vẩy nến. Trong bài viết này, chúng tôi cung cấp một cái nhìn tổng quan ngắn gọn về sự phát triển, triển khai và đánh giá TPE tại Trung tâm thủy liệu pháp Avène-Les-Bains, Pháp."} +{"idx": 1870714, "en": "The catalytic asymmetric synthesis of the anti-COVID-19 drug Remdesivir has been realized by the coupling of the P-racemic phosphoryl chloride with protected nucleoside GS441524. The chiral bicyclic imidazole catalyst used is crucial for the dynamic kinetic asymmetric transformation (DyKAT) to proceed smoothly with high reactivity and excellent stereoselectivity (96 % conv., 22:1 SP :RP ). Mechanistic studies showed that this DyKAT is a first-order visual kinetic reaction dependent on the catalyst concentration. The unique chiral bicyclic imidazole skeleton and carbamate substituent of the catalyst are both required for the racemization process, involving the phosphoryl chloride, and subsequent stereodiscriminating step. A 10 gram scale reaction was also conducted with comparably excellent results, showing its potential for industrial application.", "gemini": "Việc tổng hợp bất đối xứng xúc tác của thuốc kháng COVID-19 Remdesivir đã được thực hiện bằng cách ghép phosphoryl clorua P-racemic với nucleoside được bảo vệ GS441524. Chất xúc tác imidazol hai vòng lập thể được sử dụng rất quan trọng đối với quá trình biến đổi động học không đối xứng (DyKAT) để tiến hành trơn tru với phản ứng cao và tính chọn lọc lập thể tuyệt vời (96 % chuyển đổi, 22:1 SP :RP ). Các nghiên cứu về cơ chế cho thấy DyKAT này là phản ứng động học trực quan bậc nhất phụ thuộc vào nồng độ chất xúc tác. Bộ khung imidazol hai vòng lập thể và chất thế carbamat độc đáo của chất xúc tác đều cần thiết cho quá trình racem hóa, liên quan đến phosphoryl clorua và bước phân biệt lập thể tiếp theo. Phản ứng ở quy mô 10 gam cũng được tiến hành với kết quả tuyệt vời tương đương, cho thấy tiềm năng ứng dụng trong công nghiệp."} +{"idx": 1870715, "en": "AIMS Sexual dimorphism has been reported in the epidemiology, neurobiologic susceptibility and clinical presentation of Alzheimer's disease (AD). As poor glycaemic control is associated with increased risks of AD, we aimed to investigate whether glycaemia-related risk factors also differ between men and women, using a retrospective, sex-specific analysis of a large Chinese cohort with diabetes. MATERIALS & METHODS A total of 85,514 Chinese individuals with type 2 diabetes (T2D; 46,783 women and 38,731 men), aged ≥60 years, were identified from electronic health records and observed for incident AD. Multivariable Cox regression analysis was used to evaluate the associations with incident AD of several glycaemia-related risk factors, including severe hypoglycaemia, mean HbA1c and indices of HbA1c variability, in men and women separately. RESULTS Over a median follow-up of 6 years, women had a higher incidence of AD than men (2.3% vs. 1.2%, p < 0.001). Both men and women shared the same independent non-glycaemic clinical predictors, which included older age, lower body mass index and longer duration of diabetes. However, for glycaemia-related risk factors, we observed that severe hypoglycaemia and indices of HbA1c variability were independent predictors of incident AD in women but not in men, and the associations were irrespective of their baseline glycaemic control and duration of diabetes. CONCLUSIONS Our findings highlighted that glycaemia-related risk factors for incident AD differ between men and women with T2D. Strategies to maintain glycaemic stability and avoid severe hypoglycaemia might be especially important to preserve healthy cognition in older women with diabetes.", "gemini": "MỤC ĐÍCH Sự khác biệt về giới tính đã được báo cáo trong dịch tễ học, tính nhạy cảm thần kinh sinh học và biểu hiện lâm sàng của bệnh Alzheimer (AD). Vì kiểm soát đường huyết kém có liên quan đến nguy cơ mắc AD tăng cao, chúng tôi nhằm mục đích điều tra xem các yếu tố nguy cơ liên quan đến đường huyết có khác nhau giữa nam và nữ hay không, bằng cách sử dụng phân tích hồi cứu theo giới tính cụ thể của một nhóm người Trung Quốc mắc bệnh tiểu đường. VẬT LIỆU & PHƯƠNG PHÁP Tổng cộng 85.514 người Trung Quốc mắc bệnh tiểu đường týp 2 (T2D; 46.783 phụ nữ và 38.731 nam giới), tuổi ≥60, được xác định từ hồ sơ sức khỏe điện tử và được quan sát về AD mới mắc. Phân tích hồi quy Cox đa biến được sử dụng để đánh giá mối liên quan với AD mới mắc của một số yếu tố nguy cơ liên quan đến đường huyết, bao gồm hạ đường huyết nghiêm trọng, HbA1c trung bình và các chỉ số biến động HbA1c, ở nam và nữ riêng biệt. KẾT QUẢ Sau thời gian theo dõi trung bình là 6 năm, phụ nữ có tỷ lệ mắc AD cao hơn nam giới (2,3% so với 1,2%, p < 0,001). Cả nam và nữ đều có chung các yếu tố dự báo lâm sàng không liên quan đến đường huyết, bao gồm tuổi cao hơn, chỉ số khối cơ thể thấp hơn và thời gian mắc bệnh tiểu đường dài hơn. Tuy nhiên, đối với các yếu tố nguy cơ liên quan đến đường huyết, chúng tôi thấy rằng hạ đường huyết nghiêm trọng và các chỉ số biến động HbA1c là các yếu tố dự báo độc lập của AD mới mắc ở phụ nữ nhưng không phải ở nam giới và các mối liên quan không phụ thuộc vào mức kiểm soát đường huyết ban đầu và thời gian mắc bệnh tiểu đường. KẾT LUẬN Các phát hiện của chúng tôi nhấn mạnh rằng các yếu tố nguy cơ liên quan đến đường huyết đối với AD mới mắc khác nhau giữa nam và nữ mắc T2D. Các chiến lược để duy trì sự ổn định đường huyết và tránh hạ đường huyết nghiêm trọng có thể đặc biệt quan trọng để bảo vệ nhận thức khỏe mạnh ở phụ nữ lớn tuổi mắc bệnh tiểu đường."} +{"idx": 1870716, "en": "Grazing by livestock greatly affects the soil carbon (C) cycle in grassland ecosystems. However, the effects of grazing at different intensities and durations on the dynamics of soil C in its subsoil layers are not clearly understood. Here, we compiled data from 78 sites (in total 122 published studies) to examine the effects of varying grazing intensities and durations on soil C content at different depths for grasslands in China. Our meta-analysis revealed that grazing led to an overall decrease in soil C content and productivity of above-ground vegetation (e.g., above-ground biomass and litter) but an increase in below-ground biomass. Specifically, the effects of grazing on soil C content became less negative or even positive with increasing soil depths. An increase of soil C content was consequently found under light grazing (LG), although soil C content still decreased under moderate and heavy grazing. The increase in soil C content under LG could be largely attributed to the increase of soil C content in subsoil layers (>20 cm), despite that soil C content in surface soil layer (0-20 cm) decreased. Moreover, the magnitude of increase in soil C content under LG in subsoil layers increased with grazing duration. A possible reason of the increase in soil C content in the subsoil layers was due to the increases in below-ground biomass. Our study highlights that LG may modify the allocation of C input and promote its accumulation in subsoil layers, thus offsetting the negative impact of grazing on surface soil C content, a finding that has significant implications for C sequestration in grasslands.", "gemini": "Việc chăn thả gia súc ảnh hưởng rất lớn đến chu trình cacbon (C) của đất trong các hệ sinh thái đồng cỏ. Tuy nhiên, những tác động của chăn thả ở các cường độ và thời gian khác nhau đối với động lực của C trong đất ở các lớp đất dưới của nó vẫn chưa được hiểu rõ. Ở đây, chúng tôi đã tổng hợp dữ liệu từ 78 địa điểm (tổng cộng 122 nghiên cứu đã công bố) để kiểm tra các tác động của cường độ và thời gian chăn thả khác nhau đối với hàm lượng C trong đất ở các độ sâu khác nhau đối với đồng cỏ ở Trung Quốc. Phân tích tổng hợp của chúng tôi cho thấy chăn thả làm giảm tổng thể hàm lượng C trong đất và năng suất của thảm thực vật trên mặt đất (ví dụ: sinh khối trên mặt đất và lớp phủ) nhưng làm tăng sinh khối dưới mặt đất. Cụ thể, tác động của chăn thả đối với hàm lượng C trong đất trở nên ít tiêu cực hơn hoặc thậm chí tích cực hơn khi độ sâu của đất tăng lên. Do đó, người ta thấy hàm lượng C trong đất tăng dưới chăn thả nhẹ (LG), mặc dù hàm lượng C trong đất vẫn giảm dưới chăn thả vừa và nặng. Sự gia tăng hàm lượng C trong đất dưới LG có thể phần lớn là do sự gia tăng hàm lượng C trong đất ở các lớp đất dưới (>20 cm), mặc dù hàm lượng C trong đất ở lớp đất mặt (0-20 cm) giảm. Hơn nữa, mức độ gia tăng hàm lượng C trong đất dưới LG ở các lớp đất dưới tăng theo thời gian chăn thả. Một lý do có thể xảy ra sự gia tăng hàm lượng C trong đất ở các lớp đất dưới là do sự gia tăng sinh khối dưới mặt đất. Nghiên cứu của chúng tôi nhấn mạnh rằng LG có thể làm thay đổi sự phân bổ đầu vào C và thúc đẩy sự tích tụ của nó ở các lớp đất dưới, do đó bù đắp tác động tiêu cực của chăn thả đối với hàm lượng C trong đất mặt, một phát hiện có ý nghĩa quan trọng đối với quá trình cô lập C ở đồng cỏ."} +{"idx": 1870717, "en": "The aim of this study was to evaluate seasonal changes in basic parameters of sperm quality and freezability behaviour of ejaculates from 10 fertile heavy draft stallions. A total of 140 ejaculates were collected, processed and evaluated during both the breeding (September-November) and non-breeding seasons (April-June). Fresh semen was evaluated for volume, concentration, total spermatozoa per ejaculate, plasma membrane integrity and total sperm motility. Cryopreserved samples were evaluated for plasma membrane integrity and sperm motility by the CASA system, and for the freezability index (FI), which was defined as the decreased ratio of viability after freezing-thawing. In fresh ejaculates, only viability showed significantly higher values in the breeding than in the non-breeding season (64.0% ± 15.0% vs. 58.6% ± 12.0%, respectively; p < .05). The sperm post-thawing analysis of viability and total motility parameters showed no significant changes linked to the season. However, the FI analysis showed that the ejaculates collected in the non-breeding season had higher cryoresistance characteristics than those collected in the breeding season. Results suggest that the presence of some cryoprotective factor/s in heavy draft horse ejaculates could be modulated by seasonality, with higher protective effects in the non-breeding season.", "gemini": "Mục đích của nghiên cứu này là đánh giá những thay đổi theo mùa trong các thông số cơ bản về chất lượng tinh trùng và khả năng đông lạnh của tinh dịch từ 10 con ngựa giống nặng khỏe mạnh. Tổng cộng 140 mẫu tinh dịch đã được thu thập, xử lý và đánh giá trong cả mùa sinh sản (tháng 9-11) và mùa không sinh sản (tháng 4-6). Tinh dịch tươi được đánh giá về thể tích, nồng độ, tổng số tinh trùng trên mỗi lần xuất tinh, tính toàn vẹn của màng tế bào chất và tổng số tinh trùng động. Các mẫu bảo quản lạnh được đánh giá về tính toàn vẹn của màng tế bào chất và khả năng vận động của tinh trùng bằng hệ thống CASA và về chỉ số khả năng đông lạnh (FI), được định nghĩa là tỷ lệ sống giảm sau khi đông lạnh-rã đông. Trong tinh dịch tươi, chỉ có khả năng sống cho thấy giá trị cao hơn đáng kể trong mùa sinh sản so với mùa không sinh sản (lần lượt là 64,0% ± 15,0% so với 58,6% ± 12,0%; p < 0,05). Phân tích khả năng sống và các thông số vận động tổng thể của tinh trùng sau khi rã đông không cho thấy thay đổi đáng kể liên quan đến mùa. Tuy nhiên, phân tích FI cho thấy tinh dịch thu được trong mùa không sinh sản có đặc tính chịu lạnh cao hơn so với tinh dịch thu được trong mùa sinh sản. Kết quả cho thấy sự hiện diện của một số yếu tố bảo vệ lạnh trong tinh dịch của ngựa kéo nặng có thể bị điều chỉnh theo mùa, với hiệu quả bảo vệ cao hơn trong mùa không sinh sản."} +{"idx": 1870718, "en": "The effect of dysfunctional mitochondria in several cell pathologies has been reported in renal diseases, including diabetic nephropathy and acute kidney injury. Previous studies have reported that mitochondrial transplantation provided surprising results in myocardial and liver ischemia, as well as in Parkinson's disease. We aimed to investigate the beneficial effects of isolated mitochondria transplantation from mesenchymal stem cells (MSCs) in vivo, to mitigate renal damage that arises from doxorubicin-mediated nephrotoxicity and its action mechanism. In this study, a kidney model of doxorubicin-mediated nephrotoxicity was used and isolated mitochondria from MSCs were transferred to the renal cortex of rats. The findings showed that the rate of isolated mitochondria from MSCs maintains sufficient membrane integrity, and was associated with a beneficial renal therapeutic effect. Following doxorubicin-mediated renal injury, isolated mitochondria or vehicle infused into the renal cortex and rats were monitored for five days. This study found that mitochondrial transplantation decreased cellular oxidative stress and promoted regeneration of tubular cells after renal injury (P < .001, P = .009). Moreover, mitochondrial transplantation reduced protein accumulation of tubular cells and reversed renal deficits (P = .01, P < .001). Mitochondrial transplantation increased Bcl-2 levels, and caspase-3 levels decreased in injured renal cells (P < .015, P < .001). Our results provide a direct link between mitochondria dysfunction and doxorubicin-mediated nephrotoxicity and suggest a therapeutic effect of transferring isolated mitochondria obtained from MSCs against renal injury. To our knowledge, this study is the first study in the literature that showed good therapeutic effects of mitochondrial transplantation in a nephrotoxicity model, which is under-researched.", "gemini": "Tác động của ty thể rối loạn chức năng trong một số bệnh lý tế bào đã được báo cáo trong các bệnh về thận, bao gồm bệnh lý thận do tiểu đường và tổn thương thận cấp. Các nghiên cứu trước đây đã báo cáo rằng cấy ghép ty thể mang lại kết quả đáng ngạc nhiên trong trường hợp thiếu máu cơ tim và gan, cũng như trong bệnh Parkinson. Chúng tôi nhằm mục đích nghiên cứu các tác dụng có lợi của việc cấy ghép ty thể riêng lẻ từ các tế bào gốc trung mô (MSC) trong cơ thể sống, để giảm thiểu tổn thương thận phát sinh từ độc tính thận do doxorubicin gây ra và cơ chế hoạt động của nó. Trong nghiên cứu này, một mô hình thận về độc tính thận do doxorubicin gây ra đã được sử dụng và ty thể riêng lẻ từ MSC được chuyển đến vỏ thận của chuột. Các phát hiện cho thấy tỷ lệ ty thể riêng lẻ từ MSC duy trì tính toàn vẹn của màng đủ và có liên quan đến tác dụng điều trị có lợi cho thận. Sau khi bị tổn thương thận do doxorubicin, ty thể riêng lẻ hoặc chất mang được truyền vào vỏ thận và chuột được theo dõi trong năm ngày. Nghiên cứu này phát hiện ra rằng cấy ghép ty thể làm giảm stress oxy hóa tế bào và thúc đẩy tái tạo các tế bào ống sau khi bị tổn thương thận (P <0,001, P = 0,009). Hơn nữa, cấy ghép ty thể làm giảm sự tích tụ protein của các tế bào ống và đảo ngược tình trạng thiếu hụt thận (P = 0,01, P <0,001). Cấy ghép ty thể làm tăng mức Bcl-2 và mức caspase-3 giảm ở các tế bào thận bị tổn thương (P <0,015, P <0,001). Kết quả của chúng tôi cung cấp mối liên hệ trực tiếp giữa rối loạn chức năng ty thể và độc tính thận do doxorubicin gây ra và gợi ý một tác dụng điều trị của việc chuyển ty thể riêng lẻ thu được từ MSC chống lại tổn thương thận. Theo chúng tôi biết, nghiên cứu này là nghiên cứu đầu tiên trong tài liệu cho thấy hiệu quả điều trị tốt của việc cấy ghép ty thể trong mô hình độc tính thận, vốn đang được nghiên cứu chưa đầy đủ."} +{"idx": 1870719, "en": "Invited for the cover of this issue is Christopher Bejger and co-workers at UNC Charlotte, Columbia University, and Donghua University. The image depicts a pair of star clusters in the constellation Perseus as the structure of two metal clusters in the reported framework. Read the full text of the article at 10.1002/chem.20201215.", "gemini": "Được mời lên trang bìa của số báo này là Christopher Bejger và các cộng sự tại UNC Charlotte, Đại học Columbia và Đại học Đông Hoa. Hình ảnh mô tả một cặp cụm sao trong chòm sao Anh Tiên như cấu trúc của hai cụm kim loại trong khung được báo cáo. Đọc toàn văn bài báo tại 10.1002/chem.20201215."} +{"idx": 1870720, "en": "Estimates of marine N2 fixation range from 52 to 73 Tg N/year, of which we calculate up to 84% is from Trichodesmium based on previous measurements of nifH gene abundance and our new model of Trichodesmium growth. Here, we assess the likely effects of four major climate change-related abiotic factors on the spatiotemporal distribution and growth potential of Trichodesmium for the last glacial maximum (LGM), the present (2006-2015) and the end of this century (2100) by mapping our model of Trichodesmium growth onto inferred global surface ocean fields of pCO2 , temperature, light and Fe. We conclude that growth rate was severely limited by low pCO2 at the LGM, that current pCO2 levels do not significantly limit Trichodesmium growth and thus, the potential for enhanced growth from future increases in CO2 is small. We also found that the area of the ocean where sea surface temperatures (SST) are within Trichodesmium's thermal niche increased by 32% from the LGM to present, but further increases in SST due to continued global warming will reduce this area by 9%. However, the range reduction at the equator is likely to be offset by enhanced growth associated with expansion of regions with optimal or near optimal Fe and light availability. Between now and 2100, the ocean area of optimal SST and irradiance is projected to increase by 7%, and the ocean area of optimal SST, irradiance and iron is projected to increase by 173%. Given the major contribution of this keystone species to annual N2 fixation and thus pelagic ecology, biogeochemistry and CO2 sequestration, the projected increase in the geographical range for optimal growth could provide a negative feedback to increasing atmospheric CO2 concentrations.", "gemini": "Ước tính cố định N2 biển dao động từ 52 đến 73 Tg N/năm, trong đó chúng tôi tính toán có tới 84% là từ Trichodesmium dựa trên các phép đo trước đó về sự phong phú của gen nifH và mô hình mới của chúng tôi về sự phát triển của Trichodesmium. Ở đây, chúng tôi đánh giá những tác động có thể xảy ra của bốn yếu tố phi sinh chính liên quan đến biến đổi khí hậu đối với sự phân bố không gian và thời gian và tiềm năng tăng trưởng của Trichodesmium trong thời kỳ băng hà cực đại cuối cùng (LGM), hiện tại (2006-2015) và cuối thế kỷ này (2100) bằng cách lập bản đồ mô hình tăng trưởng Trichodesmium của chúng tôi vào các trường đại dương bề mặt toàn cầu suy ra của pCO2, nhiệt độ, ánh sáng và Fe. Chúng tôi kết luận rằng tốc độ tăng trưởng bị hạn chế nghiêm trọng bởi pCO2 thấp tại LGM, rằng nồng độ pCO2 hiện tại không hạn chế đáng kể sự tăng trưởng của Trichodesmium và do đó, tiềm năng tăng trưởng từ sự gia tăng CO2 trong tương lai là nhỏ. Chúng tôi cũng phát hiện ra rằng diện tích của đại dương nơi nhiệt độ bề mặt biển (SST) nằm trong phạm vi nhiệt của Trichodesmium đã tăng 32% từ LGM đến hiện tại, nhưng sự gia tăng thêm về SST do sự nóng lên toàn cầu liên tục sẽ làm giảm diện tích này xuống 9%. Tuy nhiên, sự giảm phạm vi ở đường xích đạo có khả năng được bù đắp bằng sự tăng trưởng mạnh mẽ liên quan đến sự mở rộng của các khu vực có sẵn Fe và ánh sáng tối ưu hoặc gần tối ưu. Từ nay đến năm 2100, diện tích đại dương có SST và độ rọi tối ưu được dự đoán sẽ tăng 7%, và diện tích đại dương có SST, độ rọi và sắt tối ưu được dự đoán sẽ tăng 173%. Với sự đóng góp chính của loài then chốt này vào quá trình cố định N2 hàng năm và do đó là sinh thái học phù du, địa hóa sinh và cô lập CO2, sự gia tăng dự kiến về phạm vi địa lý để tăng trưởng tối ưu có thể cung cấp phản hồi tiêu cực cho nồng độ CO2 trong khí quyển ngày càng tăng."} +{"idx": 1870721, "en": "Dorsal human midbrain contains two nuclei with clear laminar organization, the superior and inferior colliculi. These nuclei extend in depth between the superficial dorsal surface of midbrain and a deep midbrain nucleus, the periaqueductal gray matter (PAG). The PAG, in turn, surrounds the cerebral aqueduct (CA). This study examined the use of two depth metrics to characterize depth and thickness relationships within dorsal midbrain using the superficial surface of midbrain and CA as references. The first utilized nearest-neighbor Euclidean distance from one reference surface, while the second used a level-set approach that combines signed distances from both reference surfaces. Both depth methods provided similar functional depth profiles generated by saccadic eye movements in a functional MRI task, confirming their efficacy for delineating depth for superficial functional activity. Next, the boundaries of the PAG were estimated using Euclidean distance together with elliptical fitting, indicating that the PAG can be readily characterized by a smooth surface surrounding PAG. Finally, we used the level-set approach to measure tissue depth between the superficial surface and the PAG, thus characterizing the variable thickness of the colliculi. Overall, this study demonstrates depth-mapping schemes for human midbrain that enables accurate segmentation of the PAG and consistent depth and thickness estimates of the superior and inferior colliculi.", "gemini": "Não giữa ở người chứa hai nhân có tổ chức theo lớp rõ ràng, là đồi trên và đồi dưới. Các nhân này mở rộng theo chiều sâu giữa bề mặt lưng nông của não giữa và một nhân não giữa sâu, chất xám quanh ống dẫn nước (PAG). PAG lần lượt bao quanh ống dẫn não (CA). Nghiên cứu này đã kiểm tra việc sử dụng hai số liệu độ sâu để mô tả mối quan hệ độ sâu và độ dày trong não giữa lưng bằng cách sử dụng bề mặt nông của não giữa và CA làm tham chiếu. Phương pháp đầu tiên sử dụng khoảng cách Euclid gần nhất từ một bề mặt tham chiếu, trong khi phương pháp thứ hai sử dụng phương pháp đặt mức kết hợp các khoảng cách có dấu từ cả hai bề mặt tham chiếu. Cả hai phương pháp độ sâu đều cung cấp các cấu hình độ sâu chức năng tương tự được tạo ra bởi các chuyển động mắt theo ý muốn trong nhiệm vụ MRI chức năng, xác nhận hiệu quả của chúng trong việc phân định độ sâu cho hoạt động chức năng nông. Tiếp theo, các ranh giới của PAG được ước tính bằng cách sử dụng khoảng cách Euclid cùng với phép khớp elip, chỉ ra rằng PAG có thể dễ dàng được đặc trưng bởi một bề mặt nhẵn bao quanh PAG. Cuối cùng, chúng tôi đã sử dụng phương pháp đặt mức để đo độ sâu mô giữa bề mặt nông và PAG, do đó mô tả độ dày thay đổi của các đồi. Nhìn chung, nghiên cứu này chứng minh các lược đồ lập bản đồ độ sâu cho não giữa ở người cho phép phân đoạn chính xác PAG và ước tính độ sâu và độ dày nhất quán của các đồi trên và đồi dưới."} +{"idx": 1870722, "en": "Oral lichen planus (OLP) is a chronic inflammatory disease displaying ultrastructural disturbances in epithelial hemidesmosomes. The expression of several key hemidesmosomal components in OLP as well as in normal buccal mucosa is, however, unknown. The aim of the study was therefore to examine intracellular and extracellular components involved in hemidesmosomal attachment, in OLP (n = 20) and in normal buccal mucosa (n = 10), by immunofluorescence. In normal buccal mucosa, laminin-α3γ2, integrin-α6β4, CD151, collagen α-1(XVII) chain, and dystonin showed linear expression along the basal membrane, indicating the presence of type I hemidesmosomes. Plectin stained most epithelial cell membranes and remained unphosphorylated at S4642. In OLP, most hemidesmosomal molecules examined showed disturbed expression consisting of discontinuous increases, apicolateral location, and/or intracellular accumulation. Plectin showed S4642-phosphorylation at the basement membrane, and deposits of laminin-α3 and laminin-γ2 were found within the connective tissue. The disturbed expression of hemidesmosomal proteins in OLP indicates deficient attachment of the basal cell layer, which can contribute to detachment and cell death of basal keratinocytes seen in the disease.", "gemini": "Lichen phẳng miệng (OLP) là một bệnh viêm mãn tính biểu hiện rối loạn cấu trúc siêu vi ở các hemidesmosome biểu mô. Tuy nhiên, biểu hiện của một số thành phần hemidesmosome chính trong OLP cũng như trong niêm mạc má bình thường vẫn chưa được biết. Do đó, mục đích của nghiên cứu là kiểm tra các thành phần nội bào và ngoại bào liên quan đến sự gắn kết hemidesmosome, ở OLP (n = 20) và ở niêm mạc má bình thường (n = 10), bằng phương pháp miễn dịch huỳnh quang. Ở niêm mạc má bình thường, laminin-α3γ2, integrin-α6β4, CD151, chuỗi collagen α-1 (XVII) và dystonin biểu hiện tuyến tính dọc theo màng đáy, chỉ ra sự hiện diện của hemidesmosome loại I. Plectin nhuộm hầu hết các màng tế bào biểu mô và vẫn không phosphoryl hóa ở S4642. Trong OLP, hầu hết các phân tử hemidesmosome được kiểm tra đều biểu hiện rối loạn bao gồm tăng không liên tục, vị trí đỉnh bên và/hoặc tích tụ nội bào. Plectin biểu hiện phosphoryl hóa S4642 tại màng đáy và các lắng đọng laminin-α3 và laminin-γ2 được tìm thấy trong mô liên kết. Sự biểu hiện rối loạn của các protein hemidesmosome trong OLP chỉ ra sự gắn kết kém của lớp tế bào đáy, có thể góp phần vào sự bong tróc và chết tế bào của các tế bào sừng đáy được thấy trong bệnh."} +{"idx": 1870726, "en": "Invited for this month's cover is the group of Karen Wilson and Adam Lee at RMIT University. The image shows platinum nanoparticles and Brønsted acid sites working cooperatively to catalyse the efficient hydrodeoxygenation of phenolic lignin residues to produce sustainable biofuels. The Full Paper itself is available at 10.1002/cssc.202000764.", "gemini": "Được mời cho trang bìa tháng này là nhóm Karen Wilson và Adam Lee tại Đại học RMIT. Hình ảnh cho thấy các hạt nano bạch kim và các vị trí axit Brønsted hoạt động phối hợp để xúc tác quá trình khử oxy hóa hiệu quả các chất cặn lignin phenolic để sản xuất nhiên liệu sinh học bền vững. Bản báo cáo đầy đủ có tại 10.1002/cssc.202000764."} +{"idx": 1870727, "en": "Despite the importance of emotional intelligence, its biological mechanism is still not well understood. For this reason, we have developed a rodent detour task which requires an animal to reach a highly desired object placed directly behind a transparent barrier that blocks the direct route to the target. This apparently simple task is highly dependent on the emotional control that is necessary to inhibit prepotent and counterproductive responses driven by the sight of a desired object. The water escape detour task designed for mice enables testing the ability to solve emotionally challenging problems, as well as identification of an impairment termed perseveration. Such a maladaptive reaction to a challenging situation is characterized by difficulty in terminating an unsuccessful response, leading to persistent repetition of inappropriate behavior. This issue is important because perseveration is associated with schizophrenia, drug abuse, and aging. © 2020 Wiley Periodicals LLC. Basic Protocol: Water escape detour task Support Protocol 1: Preparation of escape platform Support Protocol 2: Preparation of the transparent barrier Alternate Protocol: Water escape detour task for testing acute effects.", "gemini": "Mặc dù trí tuệ cảm xúc rất quan trọng, nhưng cơ chế sinh học của nó vẫn chưa được hiểu rõ. Vì lý do này, chúng tôi đã phát triển một nhiệm vụ vòng vo ở loài gặm nhấm đòi hỏi một con vật phải với tới một vật rất mong muốn được đặt ngay sau một rào chắn trong suốt chặn đường trực tiếp đến mục tiêu. Nhiệm vụ đơn giản này rõ ràng phụ thuộc rất nhiều vào khả năng kiểm soát cảm xúc cần thiết để ức chế các phản ứng mạnh mẽ và phản tác dụng do việc nhìn thấy một vật mong muốn thúc đẩy. Nhiệm vụ vòng vo thoát khỏi nước được thiết kế cho chuột cho phép kiểm tra khả năng giải quyết các vấn đề khó khăn về mặt cảm xúc, cũng như xác định một khiếm khuyết được gọi là sự kiên trì. Một phản ứng không thích nghi như vậy đối với một tình huống khó khăn được đặc trưng bởi khó khăn trong việc chấm dứt một phản ứng không thành công, dẫn đến việc lặp đi lặp lại liên tục hành vi không phù hợp. Vấn đề này rất quan trọng vì sự kiên trì có liên quan đến bệnh tâm thần phân liệt, lạm dụng ma túy và lão hóa. © 2020 Wiley Periodicals LLC. Giao thức cơ bản: Nhiệm vụ vòng vo thoát khỏi nước Giao thức hỗ trợ 1: Chuẩn bị bệ thoát hiểm Giao thức hỗ trợ 2: Chuẩn bị rào chắn trong suốt Giao thức thay thế: Nhiệm vụ vòng vo thoát khỏi nước để kiểm tra các tác động cấp tính."} +{"idx": 1870728, "en": "The self-assembly of bowlic supramolecules on graphene surface is studied with single molecular sensitivity. This is achieved by incorporating a heavy metal tag in the form of a single W atom into the tip of the molecular structure, which enables the direct imaging of molecular distribution using annular dark-field scanning transmission electron microscopy (ADF-STEM) along with graphene as an electron transparent support. The bowlic molecules have nonplanar geometry, and their orientations with respect to their graphene substrate and with each other result in various packing configurations. Statistical data on intermolecular distances is obtained from numerous measurements of the bright contrast from the single metal atom tags. The analysis shows that the bowlic molecules lie sideways on the graphene surface with favorable head-to-tail stacking, rather than sitting vertically with the bowl facing toward the graphene surface. In thicker film regions, nanoscale lamellar fringes are observed, demonstrating that large-scale aligned packing extends into 3D. Image simulations and various molecular packing schemes are discussed to help interpret the ADF-STEM images and the possible range of molecular interactions occurring. These results aid the understanding of nonplanar supramolecular assemblies on van der Waals surfaces for potential applications in molecular recognition by porous films.", "gemini": "Sự tự lắp ráp của các phân tử supramolecule hình bát trên bề mặt graphene được nghiên cứu với độ nhạy của từng phân tử. Điều này đạt được bằng cách kết hợp một thẻ kim loại nặng dưới dạng một nguyên tử W đơn vào đầu cấu trúc phân tử, cho phép chụp ảnh trực tiếp sự phân bố phân tử bằng kính hiển vi điện tử truyền qua trường tối hình khuyên (ADF-STEM) cùng với graphene làm giá đỡ trong suốt cho electron. Các phân tử hình bát có hình học không phẳng và hướng của chúng so với chất nền graphene và với nhau dẫn đến nhiều cấu hình đóng gói khác nhau. Dữ liệu thống kê về khoảng cách giữa các phân tử thu được từ nhiều phép đo độ tương phản sáng từ các thẻ nguyên tử kim loại đơn. Phân tích cho thấy các phân tử hình bát nằm nghiêng trên bề mặt graphene với sự xếp chồng đầu đuôi thuận lợi, thay vì nằm thẳng đứng với chiếc bát hướng về phía bề mặt graphene. Trong các vùng màng dày hơn, người ta quan sát thấy các vân phiến nano, chứng tỏ sự đóng gói thẳng hàng trên quy mô lớn mở rộng thành 3D. Các mô phỏng hình ảnh và các lược đồ đóng gói phân tử khác nhau được thảo luận để giúp giải thích các hình ảnh ADF-STEM và phạm vi tương tác phân tử có thể xảy ra. Những kết quả này giúp hiểu được các tập hợp supramolecular không phẳng trên bề mặt van der Waals để ứng dụng tiềm năng trong nhận dạng phân tử bằng màng xốp."} +{"idx": 1870729, "en": "β-Catenin is an important co-factor which binds multiple transcriptional molecules and mediates fibrogenic signaling pathways. Its role in kidney transplantation is unknown. We quantified binding of β-catenin within renal tubular epithelial cells to transcription factors, TCF1 and FoxO1, using a proximity ligation assay in 240 transplanted kidneys, and evaluated their pathological and clinical outcomes. β-Catenin/FoxO1 binding in 1-month protocol biopsies inversely correlated with contemporaneous chronic fibrosis, subsequent inflammation. and inflammatory fibrosis (P < .001). The relative binding of β-catenin/TCF1 versus β-catenin/FoxO1 (TF ratio) was the optimal biomarker, and abnormal in diverse fibrotic transplant diseases. A high 1-month TF ratio was followed by greater tubular atrophy and interstitial fibrosis scores, cortical inflammation, renal impairment, and proteinuria at 1 year (n = 131, all P < .001). The TF ratio was associated with reduced eGFR (AUC 0.817), mild fibrosis (AUC 0.717), and moderate fibrosis (AUC 0.769) using receiver operating characteristic analysis. An independent validation cohort (n = 76) confirmed 1-month TF was associated with 12-month moderate fibrosis (15.8% vs. 2.6%, P = .047), however, not with other outcomes or 10-year graft survival, which limits generalizabilty of these findings. In summary, differential binding of β-catenin to TCF1 rather than FoxO1 in renal tubular cells was associated with the fibrogenic response in transplanted kidneys.", "gemini": "β-Catenin là một đồng yếu tố quan trọng liên kết nhiều phân tử phiên mã và trung gian các con đường truyền tín hiệu sinh xơ. Vai trò của nó trong ghép thận vẫn chưa được biết rõ. Chúng tôi định lượng sự liên kết của β-catenin trong các tế bào biểu mô ống thận với các yếu tố phiên mã, TCF1 và FoxO1, bằng cách sử dụng xét nghiệm gắn kết gần ở 240 quả thận được ghép và đánh giá kết quả bệnh lý và lâm sàng của chúng. Liên kết β-Catenin/FoxO1 trong sinh thiết theo phác đồ 1 tháng có mối tương quan nghịch với tình trạng xơ hóa mãn tính đồng thời, tình trạng viêm sau đó. và xơ hóa do viêm (P < .001). Tỷ lệ liên kết tương đối của β-Catenin/TCF1 so với β-Catenin/FoxO1 (tỷ lệ TF) là sinh học đánh dấu tối ưu và bất thường ở nhiều bệnh ghép xơ hóa khác nhau. Tỷ lệ TF 1 tháng cao tiếp theo là điểm teo ống và xơ kẽ, viêm vỏ, suy thận và protein niệu cao hơn ở 1 năm (n = 131, tất cả P < .001). Tỷ lệ TF có liên quan đến eGFR giảm (AUC 0,817), xơ hóa nhẹ (AUC 0,717) và xơ hóa vừa phải (AUC 0,769) khi sử dụng phân tích đặc điểm hoạt động của máy thu. Một nhóm xác nhận độc lập (n = 76) xác nhận TF 1 tháng có liên quan đến xơ hóa vừa phải ở tháng thứ 12 (15,8% so với 2,6%, P = .047), tuy nhiên, không liên quan đến các kết cục khác hoặc sự sống còn của ghép sau 10 năm, điều này hạn chế khả năng tổng quát của những phát hiện này. Tóm lại, sự liên kết khác biệt của β-catenin với TCF1 chứ không phải FoxO1 trong các tế bào ống thận có liên quan đến phản ứng sinh xơ ở thận được ghép."} +{"idx": 1870730, "en": "Pancytokeratins and TTF-1 are used in working up carcinomas of unknown primary and p63 is expressed in many cell lineages. We present a case of a TTF-1, p63, and cytokeratins positive small round blue cell lesion presenting in a patient with enlarged right supraclavicular lymph nodes and multiple solid pulmonary nodules. The preliminary report to the clinical team was \"suspicious for carcinoma.\" However, after a complete work up the final diagnosis of diffuse large B-cell lymphoma, nongerminal center B-cell phenotype, \"double expressor,\" was rendered (based on Han's algorithm). This case brings up significant diagnostic dilemma as some lymphoid malignancies can morphologically mimic poorly differentiated carcinoma and stain positive for carcinoma markers. Additionally, the frequently used TTF-1 SPT23 antibody clone has strong nuclear staining in rare cases of DLBCL, which is a diagnostic pitfall. To our best knowledge this is the first reported case of DLBCL staining positive for three carcinoma markers.", "gemini": "Pancytokeratin và TTF-1 được sử dụng trong quá trình tìm kiếm các ung thư biểu mô chưa biết nguyên phát và p63 được biểu hiện ở nhiều dòng tế bào. Chúng tôi trình bày một trường hợp tổn thương tế bào tròn nhỏ màu xanh dương dương tính với TTF-1, p63 và cytokeratin xuất hiện ở một bệnh nhân có hạch thượng đòn phải to và nhiều nốt phổi đặc. Báo cáo sơ bộ cho nhóm lâm sàng là \"nghi ngờ ung thư biểu mô\". Tuy nhiên, sau khi hoàn thành công tác tìm kiếm, chẩn đoán cuối cùng là u lympho B tế bào lớn lan tỏa, kiểu hình B tế bào không trung tâm mầm, \"biểu hiện kép\" (dựa trên thuật toán của Han). Trường hợp này đưa ra tình trạng khó khăn chẩn đoán đáng kể vì một số bệnh ác tính dạng lympho có thể bắt chước về mặt hình thái ung thư biểu mô biệt hóa kém và nhuộm dương tính với các dấu ấn ung thư biểu mô. Ngoài ra, dòng kháng thể TTF-1 SPT23 thường được sử dụng có nhuộm nhân mạnh trong một số trường hợp hiếm gặp của DLBCL, đây là một cạm bẫy chẩn đoán. Theo hiểu biết tốt nhất của chúng tôi, đây là trường hợp đầu tiên được báo cáo về DLBCL nhuộm dương tính với ba dấu ấn ung thư biểu mô."} +{"idx": 1870731, "en": "Succinate dehydrogenase (SDH)-deficient gastrointestinal stromal tumors (GISTs) are characterized by the lack of mutations in KIT receptor tyrosine kinase complex and platelet derived growth factor receptor-alpha (PDGFRA) that are commonly found in the majority of GISTs. SDH-deficient GISTs comprise approximately 5%-10% of all GISTs. This subset may be associated with Carney Triad and Carney-Stratakis syndrome. SDH-deficient GISTs show unique demographic, radiologic, morphologic findings, clinical behavior, and treatment response. To our knowledge, the identification and characterization of this subset of GISTs have not yet been described in the cytopathology literature. By understanding the clinical as well as the other unique features of this tumor, in addition to the rapidly evolving identification of specific molecular alterations and targeted therapies, cytopathologists may play an important role in the diagnosis and work-up of these patients to allow clinicians to better manage and treat them. We present a young female with gastric SDH-deficient GIST diagnosed by fine-needle biopsy with supporting surgical pathology follow-up and molecular confirmation. This report suggests that the diagnosis of SDH-deficient GIST can be made on cytology in the appropriate clinical setting by using cytomorphologic features and demonstrating SDH loss by IHC on the cell block. In addition, molecular testing may be possible on the cytology cell block or supernatant to confirm the diagnosis.", "gemini": "U mô đệm đường tiêu hóa (GIST) thiếu succinate dehydrogenase (SDH) được đặc trưng bởi việc thiếu đột biến ở phức hợp thụ thể tyrosine kinase KIT và thụ thể yếu tố tăng trưởng có nguồn gốc từ tiểu cầu-alpha (PDGFRA) thường thấy ở phần lớn các GIST. GIST thiếu SDH chiếm khoảng 5% -10% tổng số GIST. Nhóm này có thể liên quan đến Hội chứng ba chứng Carney và Hội chứng Carney-Stratakis. GIST thiếu SDH cho thấy các đặc điểm nhân khẩu học, hình ảnh học, hình thái, hành vi lâm sàng và đáp ứng điều trị độc đáo. Theo hiểu biết của chúng tôi, việc xác định và đặc điểm của nhóm GIST này vẫn chưa được mô tả trong tài liệu về tế bào học. Bằng cách hiểu các đặc điểm lâm sàng cũng như các đặc điểm độc đáo khác của khối u này, ngoài việc nhanh chóng xác định các biến đổi phân tử cụ thể và các liệu pháp nhắm mục tiêu, các nhà tế bào học có thể đóng một vai trò quan trọng trong việc chẩn đoán và tìm kiếm các bệnh nhân này để cho phép các bác sĩ lâm sàng quản lý và điều trị tốt hơn. Chúng tôi trình bày một phụ nữ trẻ bị GIST thiếu SDH ở dạ dày được chẩn đoán bằng sinh thiết bằng kim nhỏ với sự hỗ trợ của theo dõi bệnh lý phẫu thuật và xác nhận phân tử. Báo cáo này cho thấy chẩn đoán GIST thiếu SDH có thể được thực hiện trên tế bào học trong bối cảnh lâm sàng thích hợp bằng cách sử dụng các đặc điểm tế bào hình thái và chứng minh mất SDH bằng IHC trên khối tế bào. Ngoài ra, xét nghiệm phân tử có thể được thực hiện trên khối tế bào hoặc dịch siêu của tế bào học để xác nhận chẩn đoán."} +{"idx": 1870732, "en": "High-performance miniaturized energy storage devices have developed rapidly in recent years. Different from conventional energy storage devices, microbatteries assume the main responsibility for micropower supply, functionalization, and characterization platforms. Evolving from the essential goals for battery design of high power density, high energy density, and long lifetime, further practical demands for microbatteries (MBs) have been raised for the microfabrication technique and device design. Numerous studies have generally focused on specific aspects of the microelectrode structures or certain microfabrication techniques, while the connection from techniques to functional applications is rarely involved. This Review generally fills such blanks from an application-oriented perspective. First, some basic micromachining techniques with different compatible features are summarized. Afterward, device designs including diversified battery reaction types, configuration, and assembly are highlighted, as well as microbatteries serving powering resources or further complicated functional systems. Finally, through providing the overall design concept based on requirements in application, this Review offers innovative insights for further development of microbatteries.", "gemini": "Các thiết bị lưu trữ năng lượng thu nhỏ hiệu suất cao đã phát triển nhanh chóng trong những năm gần đây. Không giống như các thiết bị lưu trữ năng lượng thông thường, pin siêu nhỏ đảm nhận trách nhiệm chính cho nguồn cung cấp năng lượng siêu nhỏ, nền tảng chức năng hóa và đặc tính hóa. Phát triển từ các mục tiêu thiết yếu để thiết kế pin có mật độ công suất cao, mật độ năng lượng cao và tuổi thọ dài, nhu cầu thực tế hơn nữa đối với pin siêu nhỏ (MB) đã được đưa ra đối với kỹ thuật chế tạo vi mô và thiết kế thiết bị. Nhiều nghiên cứu thường tập trung vào các khía cạnh cụ thể của cấu trúc vi điện cực hoặc một số kỹ thuật chế tạo vi mô nhất định, trong khi mối liên hệ từ kỹ thuật đến ứng dụng chức năng hiếm khi được đề cập. Đánh giá này nhìn chung lấp đầy những khoảng trống đó từ góc độ hướng đến ứng dụng. Đầu tiên, một số kỹ thuật gia công vi mô cơ bản với các tính năng tương thích khác nhau được tóm tắt. Sau đó, thiết kế thiết bị bao gồm các loại phản ứng pin đa dạng, cấu hình và lắp ráp được nêu bật, cũng như các pin siêu nhỏ phục vụ nguồn cung cấp năng lượng hoặc các hệ thống chức năng phức tạp hơn nữa. Cuối cùng, thông qua việc cung cấp khái niệm thiết kế tổng thể dựa trên các yêu cầu trong ứng dụng, Đánh giá này cung cấp những hiểu biết sáng tạo để phát triển thêm các pin siêu nhỏ."} +{"idx": 1870733, "en": "Despite the recent attention for Li metal anode (LMA) with high theoretical specific capacity of ≈3860 mA h g-1 , it suffers from not enough practical energy densities and safety concerns originating from the excessive metal load, which is essential to compensate for the loss of Li sources resulting from their poor coulombic efficiencies (CEs). Therefore, the development of high-performance LMA is needed to realize anode-minimized Li metal batteries (LMBs). In this study, high-performance LMAs are produced by introducing a hierarchically nanoporous assembly (HNA) composed of functionalized onion-like graphitic carbon building blocks, several nanometers in diameter, as a catalytic scaffold for Li-metal storage. The HNA-based electrodes lead to a high Li ion concentration in the nanoporous structure, showing a high CE of ≈99.1%, high rate capability of 12 mA cm-2 , and a stable cycling behavior of more than 750 cycles. In addition, anode-minimized LMBs are achieved using a HNA that has limited Li content (≈0.13 mg cm-2 ), corresponding to 6.5% of the cathode material (commercial NCM622 (≈2 mg cm-2 )). The LMBs demonstrate a feasible electrochemical performance with high energy and power densities of ≈510 Wh kgelectrode -1 and ≈2760 W kgelectrode -1 , respectively, for more than 100 cycles.", "gemini": "Mặc dù gần đây đã chú ý đến anot kim loại Li (LMA) có dung lượng riêng lý thuyết cao ≈3860 mA h g-1 , nhưng LMA vẫn bị thiếu mật độ năng lượng thực tế và các mối lo ngại về an toàn bắt nguồn từ tải kim loại quá mức, điều này rất cần thiết để bù đắp cho sự mất nguồn Li do hiệu suất coulomb (CE) kém của chúng. Do đó, cần phát triển LMA hiệu suất cao để chế tạo pin kim loại Li có anot tối thiểu (LMB). Trong nghiên cứu này, LMA hiệu suất cao được sản xuất bằng cách đưa vào một cụm lắp ráp nano xốp theo thứ bậc (HNA) bao gồm các khối xây dựng cacbon graphit giống như hành tây được chức năng hóa, có đường kính vài nanomet, như một giàn giáo xúc tác để lưu trữ kim loại Li. Các điện cực dựa trên HNA dẫn đến nồng độ ion Li cao trong cấu trúc nano xốp, cho thấy CE cao ≈99,1%, khả năng tốc độ cao 12 mA cm-2 và hành vi chu kỳ ổn định trong hơn 750 chu kỳ. Ngoài ra, LMB có anot tối thiểu đạt được bằng cách sử dụng HNA có hàm lượng Li hạn chế (≈0,13 mg cm-2 ), tương ứng với 6,5% vật liệu catot (NCM622 thương mại (≈2 mg cm-2 )). LMB thể hiện hiệu suất điện hóa khả thi với mật độ năng lượng và công suất cao ≈510 Wh kgelectrode -1 và ≈2760 W kgelectrode -1 , tương ứng, trong hơn 100 chu kỳ."} +{"idx": 1870734, "en": "INTRODUCTION The Spanish Haematology and Haemotherapy Society organizes peripheral blood smear review scheme, focused on the evaluation of diagnostic proficiency of participants by blood cell morphology analysis. The objective was to evaluate the efficacy of this scheme as an educational tool to improve the diagnostic proficiency of the participants. METHODS During 2011-2019, 54 peripheral blood smears, alongside with patient details such as age, sex, blood cell counts and relevant clinical information, were sent to an average of 125 ± 13 laboratories per year. A number of 44 shipments were selected to analyse whether successive surveys of the same disease may lead to an improvement in the diagnostic success rate proposed by the laboratories. Participants were asked to select the most relevant morphological abnormalities, alongside the diagnostic orientation. Agreement of participant responses with RR was evaluated. RESULTS Spanish laboratories showed a diagnostic proficiency greater than 80% in acute myeloid leukaemia, including acute promyelocytic leukaemia, mature B-cell neoplasms (hairy cell leukaemia and splenic marginal zone lymphoma), chronic myeloid leukaemia, sickle cell disease, Bernard-Soulier syndrome and infectious mononucleosis. It was important to note the significant improvement over the time in the successive shipments of the same disease, with a 31% and 13% increase in their diagnostic orientation success rate for acute myeloid leukaemia and acute promyelocytic leukaemia cases, respectively, 15% for mantle cell lymphoma and 6% for sickle cell disease. CONCLUSIONS The present study provides evidence that peripheral blood smear review scheme can be a valid educational tool to improve the clinical pathologist skills in blood morphology and haematological diagnosis.", "gemini": "GIỚI THIỆU Hiệp hội Huyết học và Truyền máu Tây Ban Nha tổ chức chương trình đánh giá mẫu máu ngoại vi, tập trung vào việc đánh giá trình độ chẩn đoán của những người tham gia bằng cách phân tích hình thái tế bào máu. Mục tiêu là đánh giá hiệu quả của chương trình này như một công cụ giáo dục để nâng cao trình độ chẩn đoán của những người tham gia. PHƯƠNG PHÁP Trong giai đoạn 2011-2019, 54 mẫu máu ngoại vi, cùng với các chi tiết về bệnh nhân như tuổi, giới tính, số lượng tế bào máu và thông tin lâm sàng có liên quan, đã được gửi đến trung bình 125 ± 13 phòng xét nghiệm mỗi năm. Một số 44 lô hàng đã được chọn để phân tích xem các cuộc khảo sát liên tiếp về cùng một bệnh có thể dẫn đến cải thiện tỷ lệ thành công chẩn đoán do các phòng xét nghiệm đề xuất hay không. Những người tham gia được yêu cầu chọn những bất thường về hình thái có liên quan nhất, cùng với định hướng chẩn đoán. Sự nhất trí giữa các phản hồi của người tham gia với RR đã được đánh giá. KẾT QUẢ Các phòng xét nghiệm Tây Ban Nha cho thấy trình độ chẩn đoán cao hơn 80% đối với bệnh bạch cầu myeloid cấp tính, bao gồm bệnh bạch cầu tiền tủy cấp tính, tân sinh tế bào B trưởng thành (bệnh bạch cầu tế bào lông và u lympho vùng biên lá lách), bệnh bạch cầu myeloid mạn tính, bệnh hồng cầu hình liềm, hội chứng Bernard-Soulier và bệnh tăng bạch cầu đơn nhân truyền nhiễm. Điều quan trọng cần lưu ý là sự cải thiện đáng kể theo thời gian trong các lô hàng liên tiếp của cùng một bệnh, với tỷ lệ thành công trong định hướng chẩn đoán tăng 31% và 13% tương ứng đối với các trường hợp bệnh bạch cầu myeloid cấp tính và bệnh bạch cầu tiền tủy cấp tính, 15% đối với u lympho tế bào áo choàng và 6% đối với bệnh hồng cầu hình liềm. KẾT LUẬN Nghiên cứu hiện tại cung cấp bằng chứng cho thấy chương trình đánh giá mẫu máu ngoại vi có thể là một công cụ giáo dục hợp lệ để nâng cao các kỹ năng của bác sĩ giải phẫu bệnh về hình thái máu và chẩn đoán huyết học."} +{"idx": 1870735, "en": "BACKGROUND Rapid on-site evaluation (ROSE) is a valuable tool for specimen adequacy assessment in thyroid ultrasound (US)-guided fine-needle aspiration (US-guided FNA). To reduce the risk of nondiagnostic samples, additional needle passes may be needed at ROSE to ensure adequate sampling. Recommendations regarding the number of aspirates to ensure specimen adequacy are not well defined. Furthermore, there are limited data regarding nodule characteristics that may require increased sampling. In this study, we investigate conditions associated with requiring more than three needle passes during ROSE. METHODS A retrospective quality review of all patients who underwent US-guided thyroid FNA by a single board-certified radiologist over a 1-year period was performed. A total of 122 patients were identified: 70 with three passes performed and 52 with more than three passes to achieve adequacy. RESULT Our data demonstrate that large nodules (≥3 cm) were more likely than small nodules (≤1.1 cm) to require more than three passes to achieve adequacy. If a nodule was predominantly cystic or mixed cystic and solid, the sample was often adequate with only three passes. In cases of thyroiditis or nodules suspicious or diagnostic of neoplasia, there is a trend to require only three passes for adequacy. CONCLUSION On the basis of the data presented in this study, cytopathologists should be prepared for the potential need to obtain additional needle passes in larger (≥3 cm) nodules and provide reassurance to patients that this is an anticipated finding for these larger nodules.", "gemini": "BỐI CẢNH Đánh giá nhanh tại chỗ (ROSE) là một công cụ có giá trị để đánh giá tính đầy đủ của mẫu vật trong siêu âm tuyến giáp (US) hướng dẫn chọc hút kim nhỏ (US-guided FNA). Để giảm nguy cơ mẫu không chẩn đoán được, có thể cần thêm các lần chọc kim tại ROSE để đảm bảo lấy mẫu đầy đủ. Các khuyến nghị về số lần hút để đảm bảo tính đầy đủ của mẫu vật chưa được xác định rõ ràng. Hơn nữa, có dữ liệu hạn chế về các đặc điểm của nốt có thể yêu cầu lấy mẫu nhiều hơn. Trong nghiên cứu này, chúng tôi tìm hiểu các tình trạng liên quan đến việc yêu cầu nhiều hơn ba lần chọc kim trong quá trình ROSE. PHƯƠNG PHÁP Đã thực hiện đánh giá chất lượng hồi cứu tất cả bệnh nhân được một bác sĩ chuyên khoa được chứng nhận tiến hành FNA tuyến giáp hướng dẫn bằng US trong thời gian 1 năm. Tổng cộng có 122 bệnh nhân được xác định: 70 bệnh nhân được thực hiện ba lần chọc và 52 bệnh nhân được thực hiện nhiều hơn ba lần chọc để đạt được tính đầy đủ. KẾT QUẢ Dữ liệu của chúng tôi chứng minh rằng các nốt lớn (≥3 cm) có khả năng yêu cầu nhiều hơn ba lần chọc để đạt được tính đầy đủ hơn các nốt nhỏ (≤1,1 cm). Nếu một nốt chủ yếu là nang hoặc hỗn hợp nang và đặc, thì mẫu thường đầy đủ chỉ với ba lần chọc. Trong trường hợp viêm tuyến giáp hoặc các nốt đáng ngờ hoặc chẩn đoán tân sinh, có xu hướng chỉ cần ba lần chọc để đạt được tính đầy đủ. KẾT LUẬN Trên cơ sở dữ liệu được trình bày trong nghiên cứu này, các nhà tế bào bệnh học nên chuẩn bị cho khả năng cần thực hiện thêm các lần chọc kim ở các nốt lớn hơn (≥3 cm) và trấn an bệnh nhân rằng đây là một phát hiện dự đoán đối với những nốt lớn hơn này."} +{"idx": 1870736, "en": "BACKGROUND Neurodevelopmental disorders (NDDs) are a group of disorders with high genetic and phenotypic heterogeneities. The 16p11.2 microdeletion has been implicated as an important genetic risk factor for NDDs. METHODS Multiple genetic tests were used to detect the 16p11.2 microdeletion from 918 Chinese children with NDDs. Targeted sequencing of genes in the 16p11.2 interval was performed in all carriers of the 16p11.2 microdeletion, and whole-genome expression profiling analysis was performed for the patient carriers and normal carriers in their intra-family. RESULTS Three patients carrying the 16p11.2 microdeletion were screened out, indicating a frequency of 0.33% for the 16p11.2 microdeletion in this cohort. We reviewed the neurodevelopmental trajectories of the 16p11.2 microdeletion carriers from childhood to puberty and confirmed that this microdeletion was associated with abnormal neurodevelopment, with varied neurodevelopmental phenotypes. A differential PRRT2 genotype (rs10204, T>C) was identified between patients and normal carriers of the 16p11.2 microdeletion. Moreover, the determination of differential whole-genome expression profiling demonstrated the destruction of the top-ranked network in neurogenesis and accounted for observation of abnormal neurodevelopmental phenotypes in the 16p11.2 microdeletion carriers. CONCLUSIONS We have provided the frequency of the 16p11.2 microdeletion in a Chinese pediatric NDD cohort with a variable NDD phenotype from childhood to puberty, which is useful for Chinese geneticists/pediatricians to conduct the 16p11.2 microdeletion testing in children with NDDs.", "gemini": "BỐI CẢNH Các rối loạn phát triển thần kinh (NDD) là một nhóm các rối loạn có tính dị hợp di truyền và kiểu hình cao. Sự vi mất đoạn 16p11.2 đã được xác định là một yếu tố nguy cơ di truyền quan trọng đối với NDD. PHƯƠNG PHÁP Đã sử dụng nhiều xét nghiệm di truyền để phát hiện vi mất đoạn 16p11.2 từ 918 trẻ em Trung Quốc mắc NDD. Trình tự mục tiêu của các gen trong khoảng 16p11.2 đã được thực hiện ở tất cả những người mang vi mất đoạn 16p11.2 và phân tích hồ sơ biểu hiện toàn bộ hệ gen đã được thực hiện đối với những người mang bệnh và người mang bình thường trong gia đình của họ. KẾT QUẢ Ba bệnh nhân mang vi mất đoạn 16p11.2 đã được sàng lọc, cho thấy tần suất vi mất đoạn 16p11.2 là 0,33% trong nhóm này. Chúng tôi đã xem xét các quỹ đạo phát triển thần kinh của những người mang vi mất đoạn 16p11.2 từ thời thơ ấu đến tuổi dậy thì và xác nhận rằng vi mất đoạn này có liên quan đến sự phát triển thần kinh bất thường, với các kiểu hình phát triển thần kinh khác nhau. Một kiểu gen PRRT2 khác biệt (rs10204, T>C) đã được xác định giữa những bệnh nhân và những người mang bình thường của vi mất đoạn 16p11.2. Hơn nữa, việc xác định hồ sơ biểu hiện toàn bộ hệ gen khác biệt đã chứng minh sự phá hủy của mạng lưới xếp hạng hàng đầu trong quá trình sinh thần kinh và giải thích cho việc quan sát thấy các kiểu hình phát triển thần kinh bất thường ở những người mang vi mất đoạn 16p11.2. KẾT LUẬN Chúng tôi đã cung cấp tần suất vi mất đoạn 16p11.2 trong một nhóm NDD nhi khoa Trung Quốc có kiểu hình NDD thay đổi từ thời thơ ấu đến tuổi dậy thì, điều này rất hữu ích cho các nhà di truyền học/bác sĩ nhi khoa Trung Quốc tiến hành xét nghiệm vi mất đoạn 16p11.2 ở trẻ em mắc NDD."} +{"idx": 1870737, "en": "Functional mitral regurgitation in the setting of an enlarged heart is challenging to repair surgically with an annular approach, and the need to develop subannular and ventricular approaches is recognized yet unrealized because of the lack of models for investigations. In this study, we report a novel model of functional mitral regurgitation induced by left ventricular thinning and distension in pig hearts. Seven pig hearts were explanted at a local slaughterhouse, and left ventricular distension induced by thinning the ventricular myocardium by 60-65% of its original thickness. Distension of the thinned hearts with a 120 mmHg column confirmed significant left ventricular dilatation and mitral valve tethering. These hearts were then mounted into a pulsatile flow model and animated at 120 mmHg left ventricular pressure, 5 L/min cardiac output at 70 beats/min. Echocardiography was used to assess valvular kinematics and hemodynamics. Left ventricular wall thickness reduced by 60.5% ± 10.1% at the basal plane, 64.8% ± 11.3% at the equatorial plane, and 64.0% ± 11.4% at the apical plane after thinning. Upon distension, ventricular volumes increased by 852.4% ± 639.8% after left ventricular thinning, with an 89.5% ± 33.9% increase in sphericity index. Mitral valve systolic tenting height increased from 7.92 ± 2.06 to 15.02 ± 3.89 mm, systolic tethering area increased from 130.7 ± 38.2 to 409.9 ± 124.6 mm and an average mitral regurgitation fraction of 24.4% ± 16.6% was measured. In a case study, use of multimodality imaging to test the efficacy of transcatheter mitral devices was confirmed. Ventricular wall thinning leading to passive left ventricular distension and dilatation is a reproducible ex vivo model of mitral valve tethering and functional mitral regurgitation, which in combination with multimodality imaging provides a good simulation model.", "gemini": "Hở van hai lá chức năng trong trường hợp tim to là một thách thức trong việc sửa chữa phẫu thuật bằng phương pháp tiếp cận vòng van, và nhu cầu phát triển các phương pháp tiếp cận dưới vòng van và thất đã được công nhận nhưng vẫn chưa được thực hiện vì thiếu các mô hình để nghiên cứu. Trong nghiên cứu này, chúng tôi báo cáo một mô hình mới về hở van hai lá chức năng do mỏng và giãn thất trái ở tim lợn. Bảy quả tim lợn đã được lấy ra tại một lò giết mổ địa phương và gây giãn thất trái bằng cách làm mỏng cơ tim thất 60-65% so với độ dày ban đầu. Việc giãn những quả tim mỏng với cột 120 mmHg xác nhận sự giãn nở đáng kể của thất trái và van hai lá bị giật. Sau đó, những quả tim này được gắn vào một mô hình dòng chảy xung và hoạt động ở áp suất thất trái 120 mmHg, lưu lượng tim 5 lít/phút ở nhịp 70 lần/phút. Siêu âm tim được sử dụng để đánh giá động học van và huyết động học. Độ dày thành thất trái giảm 60,5% ± 10,1% ở mặt phẳng nền, 64,8% ± 11,3% ở mặt phẳng xích đạo và 64,0% ± 11,4% ở mặt phẳng đỉnh sau khi làm mỏng. Khi giãn, thể tích thất tăng 852,4% ± 639,8% sau khi làm mỏng thất trái, với chỉ số hình cầu tăng 89,5% ± 33,9%. Chiều cao lều tâm thu của van hai lá tăng từ 7,92 ± 2,06 lên 15,02 ± 3,89 mm, diện tích giật tâm thu tăng từ 130,7 ± 38,2 lên 409,9 ± 124,6 mm và tỷ lệ hở van hai lá trung bình là 24,4% ± 16,6% đã được đo. Trong một nghiên cứu trường hợp, việc sử dụng hình ảnh đa phương thức để kiểm tra hiệu quả của các thiết bị van hai lá qua ống thông đã được xác nhận. Làm mỏng thành thất dẫn đến giãn và giãn thất trái thụ động là một mô hình ngoại sinh tái tạo được của van hai lá bị giật và hở van hai lá chức năng, khi kết hợp với hình ảnh đa phương thức cung cấp một mô hình mô phỏng tốt."} +{"idx": 1870738, "en": "We describe the experience with biventricular HeartWare VAD (HVAD) support in the pediatric population. As of May 2017, using the Heartware database, 11 centers were identified. Seven centers participated providing information for 10 patients (four females, six males). Median age at the time of implantation was 12.7 ± 4.6years (5.3-6.9), median body surface area was 1.56. The indications for biventricular assist device (BiVAD) support included: myocarditis (n = 1), cardiomyopathy (n = 8), and one posttransplant heart failure. Six patients had a primary BiVAD implantation. Out of 10 patients with BiVAD, five were transplanted, one is ongoing (postoperatively day 207), and none were weaned from the device. Two patients (20%) were discharged from hospital on BiVAD support. Median support time was 52 days (16-235). The overall success rate was 60% surviving to transplant (median support time: 51 days) or are ongoing. Reasons for death included bleeding (n = 2), intracerebral hemorrhage (n = 1), and multisystem organ failure (n = 1). Eight out of 10 patients had at least one major complication, that is, major bleeding requiring re-operation (n = 6), severe neurologic injury (n = 1), and pump thrombosis in two patients requiring device exchange (n = 1) or thrombolysis therapy (n = 1). BiVAD HVAD implantation in children is a rare procedure, with high mortality and low discharge rates. Bleeding requiring re-operation was the most common postoperative complication, despite fresh sternotomies in the majority of patients. The risk and benefit ratio of such an approach should be carefully reviewed, and compared with the standard strategy using the Berlin EXCOR.", "gemini": "Chúng tôi mô tả kinh nghiệm hỗ trợ VAD (HVAD) HeartWare hai tâm thất ở nhóm trẻ em. Tính đến tháng 5 năm 2017, sử dụng cơ sở dữ liệu Heartware, 11 trung tâm đã được xác định. Bảy trung tâm đã tham gia cung cấp thông tin cho 10 bệnh nhân (bốn nữ, sáu nam). Độ tuổi trung bình tại thời điểm cấy ghép là 12,7 ± 4,6 tuổi (5,3-6,9), diện tích bề mặt cơ thể trung bình là 1,56. Các chỉ định hỗ trợ thiết bị hỗ trợ hai tâm thất (BiVAD) bao gồm: viêm cơ tim (n = 1), bệnh cơ tim (n = 8) và một trường hợp suy tim sau ghép. Sáu bệnh nhân đã cấy ghép BiVAD chính. Trong số 10 bệnh nhân được ghép BiVAD, năm bệnh nhân đã được ghép, một bệnh nhân đang tiếp tục (ngày thứ 207 sau phẫu thuật) và không có bệnh nhân nào cai được máy. Hai bệnh nhân (20%) đã được xuất viện khỏi bệnh viện khi được hỗ trợ BiVAD. Thời gian hỗ trợ trung bình là 52 ngày (16-235). Tỷ lệ thành công chung là 60% sống sót sau ghép (thời gian hỗ trợ trung bình: 51 ngày) hoặc đang tiếp tục. Các lý do tử vong bao gồm chảy máu (n = 2), xuất huyết não (n = 1) và suy đa cơ quan (n = 1). Tám trong số 10 bệnh nhân có ít nhất một biến chứng chính, đó là chảy máu lớn cần phẫu thuật lại (n = 6), tổn thương thần kinh nghiêm trọng (n = 1) và huyết khối máy bơm ở hai bệnh nhân cần đổi máy (n = 1) hoặc điều trị tiêu huyết khối (n = 1). Cấy ghép BiVAD HVAD ở trẻ em là một thủ thuật hiếm, có tỷ lệ tử vong cao và tỷ lệ xuất viện thấp. Chảy máu cần phẫu thuật lại là biến chứng hậu phẫu phổ biến nhất, mặc dù hầu hết bệnh nhân đều được mở xương ức. Tỷ lệ rủi ro và lợi ích của phương pháp tiếp cận như vậy nên được xem xét cẩn thận và so sánh với chiến lược tiêu chuẩn sử dụng EXCOR Berlin."} +{"idx": 1870739, "en": "INTRODUCTION Long-term effects of physical activity and television (TV) viewing on mortality have been inferred from observational studies. The associations observed do not allow for inferences about the effects of population interventions and could be subject to bias due to time-varying confounding. METHODS Using data from the Australian Diabetes, Obesity and Lifestyle Study, collected in 1999-2000 (T0), 2004-2005 (T1), and 2011-2012 (T2), we applied the parametric g-formula to estimate cumulative risks of death under hypothetical interventions on physical activity and/or TV viewing determined from self-report while adjusting for time-varying confounding. RESULTS In the 6377 participants followed up for 13 yr from 2004 to 2005 to death or censoring in 2017, 781 participants died. The observed cumulative risk of death was 12.2%. The most effective hypothetical intervention was to increase weekly physical activity to >300 min (risk ratio (RR), 0.66 (0.46-0.86) compared with a \"worst-case\" scenario; RR, 0.83 (0.73-0.94) compared with no intervention). Reducing daily TV viewing to <2 h in addition to physical activity interventions did not show added survival benefits. Reducing TV viewing alone was least effective in reducing mortality (RR, 0.85 (0.60-1.10) compared with the worst-case scenario; RR, 1.06 (0.93-1.20) compared with no intervention). CONCLUSIONS Our findings suggested that sustained interventions to increase physical activity could lower all-cause mortality over a 13-yr period, and there might be limited gain from intervening to reduce TV viewing time in a relatively healthy population.", "gemini": "GIỚI THIỆU Các tác động dài hạn của hoạt động thể chất và xem tivi (TV) đối với tỷ lệ tử vong đã được suy ra từ các nghiên cứu quan sát. Các mối liên quan được quan sát không cho phép suy ra về tác động của các biện pháp can thiệp của dân số và có thể bị thiên lệch do nhiễu loạn thay đổi theo thời gian. PHƯƠNG PHÁP Sử dụng dữ liệu từ Nghiên cứu về bệnh tiểu đường, béo phì và lối sống của Úc, được thu thập vào năm 1999-2000 (T0), 2004-2005 (T1) và 2011-2012 (T2), chúng tôi đã áp dụng công thức g tham số để ước tính rủi ro tử vong tích lũy theo các biện pháp can thiệp giả định về hoạt động thể chất và/hoặc xem TV được xác định từ báo cáo tự nêu trong khi điều chỉnh nhiễu loạn thay đổi theo thời gian. KẾT QUẢ Trong số 6377 người tham gia được theo dõi trong 13 năm từ năm 2004 đến 2005 đến khi tử vong hoặc kiểm duyệt vào năm 2017, 781 người tham gia đã tử vong. Rủi ro tử vong tích lũy được quan sát là 12,2%. Biện pháp can thiệp giả định hiệu quả nhất là tăng hoạt động thể chất hàng tuần lên> 300 phút (tỷ lệ rủi ro (RR), 0,66 (0,46-0,86) so với trường hợp \"xấu nhất\"; RR, 0,83 (0,73-0,94) so với không can thiệp). Giảm thời gian xem TV hàng ngày xuống dưới 2 giờ ngoài các biện pháp can thiệp về hoạt động thể chất không cho thấy lợi ích sống còn được cộng thêm. Chỉ riêng việc giảm thời gian xem TV là biện pháp kém hiệu quả nhất trong việc giảm tỷ lệ tử vong (RR, 0,85 (0,60-1,10) so với trường hợp xấu nhất; RR, 1,06 (0,93-1,20) so với không can thiệp). KẾT LUẬN Các phát hiện của chúng tôi cho thấy rằng các biện pháp can thiệp bền vững để tăng cường hoạt động thể chất có thể làm giảm tỷ lệ tử vong toàn nguyên nhân trong giai đoạn 13 năm và có thể có lợi ích hạn chế khi can thiệp để giảm thời gian xem TV trong nhóm dân số tương đối khỏe mạnh."} +{"idx": 1870740, "en": "OBJECTIVES Suicide is a leading cause of adolescent death, and emergency department (ED) visits are recognized as an opportunity to identify at-risk youth. For patients screening positive for mental health concerns, we implemented a quality improvement initiative to enhance documentation of results and interventions in the ED, increase communication between the ED and primary care providers (PCPs), and increase PCP follow-up. METHODS Interventions included education, feedback, and an alert in our electronic health record. Completion of a Behavioral Health Screen (BHS-ED) initiates an alert that reminds ED providers how to document and communicate results and needed follow-up to the PCP. We reviewed a random monthly sample of ED charts for adolescents 14 to 19 years old presenting with nonpsychiatric complaints who screened positive for severe depression or suicidality. Outcome measures included documentation of BHS-ED results in the ED note, communication of positive results to the PCP, PCP follow-up of results, and ED return visits. RESULTS Documentation of BHS-ED results increased from 73% at baseline to 88% of patients after the intervention. For patients discharged from the ED with nonpsychiatric chief complaints, communication to PCPs increased from 1% at baseline to 40% during the final 3 months of the study. When PCP communication occurred, 67% of in-network PCPs followed up with patients versus 5% when no communication took place from the ED. CONCLUSIONS A multifaceted intervention including education and an electronic health record alert improved ED documentation, communication, and PCP follow-up of issues identified during ED-based mental health screens.", "gemini": "MỤC TIÊU Tự tử là nguyên nhân tử vong hàng đầu ở thanh thiếu niên và các chuyến thăm phòng cấp cứu (ED) được công nhận là cơ hội để xác định những thanh thiếu niên có nguy cơ. Đối với những bệnh nhân có kết quả sàng lọc dương tính với các vấn đề về sức khỏe tâm thần, chúng tôi đã triển khai một sáng kiến cải thiện chất lượng để nâng cao việc ghi chép kết quả và các biện pháp can thiệp trong ED, tăng cường giao tiếp giữa ED và các nhà cung cấp dịch vụ chăm sóc chính (PCP) và tăng cường theo dõi của PCP. PHƯƠNG PHÁP Các biện pháp can thiệp bao gồm giáo dục, phản hồi và cảnh báo trong hồ sơ sức khỏe điện tử của chúng tôi. Hoàn thành Màn hình sức khỏe hành vi (BHS-ED) sẽ khởi động cảnh báo nhắc nhở các nhà cung cấp ED cách ghi chép và truyền đạt kết quả và theo dõi cần thiết cho PCP. Chúng tôi đã xem xét một mẫu biểu đồ ED hàng tháng ngẫu nhiên đối với thanh thiếu niên từ 14 đến 19 tuổi đến khám với các khiếu nại không phải về tâm thần có kết quả sàng lọc dương tính với trầm cảm nặng hoặc tự tử. Các biện pháp kết quả bao gồm ghi chép kết quả BHS-ED trong ghi chú ED, thông báo kết quả dương tính cho PCP, PCP theo dõi kết quả và các lần tái khám ED. KẾT QUẢ Ghi chép kết quả BHS-ED tăng từ 73% lúc ban đầu lên 88% bệnh nhân sau khi can thiệp. Đối với những bệnh nhân được xuất viện khỏi ED với các khiếu nại chính không phải về tâm thần, việc thông báo cho PCP đã tăng từ 1% lúc ban đầu lên 40% trong 3 tháng cuối của nghiên cứu. Khi có thông báo cho PCP, 67% PCP trong mạng đã theo dõi bệnh nhân so với 5% khi không có thông báo từ ED. KẾT LUẬN Một biện pháp can thiệp nhiều mặt bao gồm giáo dục và cảnh báo hồ sơ sức khỏe điện tử đã cải thiện việc ghi chép của ED, giao tiếp và theo dõi của PCP đối với các vấn đề được xác định trong quá trình sàng lọc sức khỏe tâm thần dựa trên ED."} +{"idx": 1870741, "en": "Diabetic retinopathy (DR), as the most frequent microvascular complication of diabetes mellitus (DM), causes vision loss and blindness in adults worldwide with increasing incidence. MicroRNAs (miRNAs) are involved in the regulation of DR. However, the role of miR-542-5p is still unknown. Here, we demonstrate that miR-542-5p is down-regulated in patients with DR and in high-glucose (HG)-treated retinal pigment epithelial cells. Moreover, miR-542-5p overexpression inhibits apoptosis in retinal pigment epithelial cells exposed to HG. The interaction between miR-542-5p and co-activator-associated arginine methyltransferase 1 (CARM1) is confirmed. MiR-542-5p mimics decrease the CARM1 level and miR-542-5p inhibitor increases the CARM1 level. Additionally, CARM1 overexpression promotes the miR-542-5p-mediated apoptosis in HG-treated retinal pigment epithelial cells. In summary, the data suggest that miR-542-5p may suppress apoptosis in retinal pigment epithelial cells via targeting CARM1, which provides a new therapeutic target for the treatment of patients with DR.", "gemini": "Bệnh võng mạc do tiểu đường (DR), là biến chứng vi mạch phổ biến nhất của bệnh tiểu đường (ĐT), gây mất thị lực và mù lòa ở người lớn trên toàn thế giới với tỷ lệ mắc bệnh ngày càng tăng. Các phân tử microRNA (miRNA) tham gia vào quá trình điều hòa DR. Tuy nhiên, vai trò của miR-542-5p vẫn chưa được biết rõ. Ở đây, chúng tôi chứng minh rằng miR-542-5p bị điều hòa xuống ở những bệnh nhân mắc DR và ở các tế bào biểu mô sắc tố võng mạc được xử lý bằng nồng độ glucose cao (HG). Hơn nữa, việc tăng biểu hiện quá mức miR-542-5p ức chế quá trình apoptosis ở các tế bào biểu mô sắc tố võng mạc tiếp xúc với HG. Sự tương tác giữa miR-542-5p và methyltransferase 1 liên kết đồng hoạt hóa arginine (CARM1) đã được xác nhận. Các phân tử giả miR-542-5p làm giảm mức CARM1 và chất ức chế miR-542-5p làm tăng mức CARM1. Ngoài ra, việc tăng biểu hiện quá mức CARM1 thúc đẩy quá trình apoptosis do miR-542-5p trung gian ở các tế bào biểu mô sắc tố võng mạc được xử lý bằng HG. Tóm lại, dữ liệu cho thấy miR-542-5p có thể ức chế quá trình apoptosis ở các tế bào biểu mô sắc tố võng mạc thông qua việc nhắm mục tiêu vào CARM1, qua đó cung cấp một mục tiêu điều trị mới cho việc điều trị bệnh nhân mắc DR."} +{"idx": 1870742, "en": "OBJECTIVE To determine whether Latin Americans who have undergone assisted reproduction techniques would donate embryos. METHODS This is a multinational cross-sectional study, involving 602 patients. We invited the Latin American Network of Assisted Reproduction centers. Those who accepted received the instrument distributed among the patients who agreed by signing the informed consent form. In total, 261 men and 341 women participated from seven countries. RESULTS Patients would donate their embryos as follows: treatment with embryonic stem cells (73.6%), heterosexual couples (63.8%), Assisted Reproduction Techniques (ART) research (57%), scientific or basic research (55.3%), research with embryonic stem cells (55.2%), premenopausal women (53.8%), single women (45.1%), people with disabilities (25.4%), lesbians (25.3%), menopausal women (25.2%), lesbian couples (24.6%), gay couples (19.6%), senile women (15.1%). CONCLUSIONS The results favor embryos donation for research purposes, and a little less for clinical purposes, contrary to what was thought in qualitative studies conducted among Latin American populations.", "gemini": "MỤC TIÊU Xác định xem những người Mỹ Latinh đã trải qua các kỹ thuật hỗ trợ sinh sản có hiến phôi hay không. PHƯƠNG PHÁP Đây là một nghiên cứu cắt ngang đa quốc gia, có sự tham gia của 602 bệnh nhân. Chúng tôi đã mời Mạng lưới các trung tâm hỗ trợ sinh sản của Mỹ Latinh. Những người chấp nhận đã nhận được công cụ phân phối cho những bệnh nhân đồng ý bằng cách ký vào mẫu đơn đồng ý có thông báo. Tổng cộng có 261 nam giới và 341 phụ nữ tham gia từ bảy quốc gia. KẾT QUẢ Bệnh nhân sẽ hiến phôi của họ như sau: điều trị bằng tế bào gốc phôi (73,6%), các cặp đôi dị tính (63,8%), nghiên cứu Kỹ thuật hỗ trợ sinh sản (ART) (57%), nghiên cứu khoa học hoặc cơ bản (55,3%), nghiên cứu bằng tế bào gốc phôi (55,2%), phụ nữ tiền mãn kinh (53,8%), phụ nữ độc thân (45,1%), người khuyết tật (25,4%), đồng tính nữ (25,3%), phụ nữ mãn kinh (25,2%), cặp đồng tính nữ (24,6%), cặp đồng tính nam (19,6%), phụ nữ già yếu (15,1%). KẾT LUẬN Kết quả ủng hộ việc hiến phôi cho mục đích nghiên cứu và ít hơn một chút cho mục đích lâm sàng, trái ngược với những gì đã nghĩ trong các nghiên cứu định tính được thực hiện trong quần thể người Mỹ Latinh."} +{"idx": 1870743, "en": "The article describes novel electrochromic materials (ECMs) that are based on a monolayer consisting of two or three isostructural metal complexes of 4'-(pyridin-4-yl)-2,2':6',2''-terpyridine simultaneously deposited on surface-enhanced support. The support was made by screen printing of indium tin oxide (ITO) nanoparticles on ITO-glass and has a surface area sufficient for a monolayer to give color visible to the naked eye. The ability to separately electrochemically address the oxidation state of the metal centers on the surface (i.e., Co2+/Co3+, Os2+/Os3+, and Fe2+/Fe3+) provides an opportunity to achieve several distinct color-to-color transitions, thus opening the door for constructing monolayer-based multicolor ECMs.", "gemini": "Bài báo mô tả các vật liệu điện sắc mới (ECM) dựa trên một lớp đơn phân tử bao gồm hai hoặc ba phức chất kim loại đẳng cấu của 4'-(pyridin-4-yl)-2,2':6',2''-terpyridine lắng đọng đồng thời trên bề mặt được tăng cường. Giá đỡ được tạo ra bằng cách in lụa các hạt nano oxit thiếc indium (ITO) trên kính ITO và có diện tích bề mặt đủ để một lớp đơn phân tử tạo ra màu sắc có thể nhìn thấy bằng mắt thường. Khả năng giải quyết riêng biệt trạng thái oxy hóa của các trung tâm kim loại trên bề mặt (tức là Co2+/Co3+, Os2+/Os3+ và Fe2+/Fe3+) tạo cơ hội để đạt được một số chuyển đổi màu sắc riêng biệt, do đó mở ra cánh cửa để chế tạo các ECM nhiều màu dựa trên lớp đơn phân tử."} +{"idx": 1870744, "en": "A concentrated aluminum chloride (AlCl3)-diglyme (G2) electrolyte is used to prepare hard and corrosion-resistant aluminum (Al) electrodeposited films. The Al electrodeposits obtained from the electrolyte with an AlCl3/G2 molar ratio x = 0.4 showed a void-free microstructure composed of spherical particles, in stark contrast to flake-like morphologies with micro-voids for lower x. Neutral complexes rarely exist in the x = 0.4 electrolyte, resulting in a relatively high conductivity despite the high concentration and high viscosity. Nanoindentation measurements for the Al deposits with >99% purity revealed that the nanohardness was 2.86 GPa, three times higher than that for Al materials produced through electrodeposition from a well-known ionic liquid bath or through severe plastic deformation. Additionally, the void-free Al deposits had a <100> preferential crystal orientation, which accounted for better resistance to free corrosion and pitting corrosion. Discussions about the compact microstructure and <100> crystal orientation of deposits obtained only from the x = 0.4 concentrated electrolyte are also presented.", "gemini": "Một chất điện phân nhôm clorua (AlCl3)-diglyme (G2) cô đặc được sử dụng để chế tạo các màng điện cực nhôm (Al) cứng và chống ăn mòn. Các lớp mạ điện Al thu được từ chất điện phân có tỷ lệ mol AlCl3/G2 x = 0,4 cho thấy vi cấu trúc không có lỗ rỗng bao gồm các hạt hình cầu, trái ngược hẳn với các hình thái giống như vảy có lỗ rỗng nhỏ đối với x thấp hơn. Các phức chất trung tính hiếm khi tồn tại trong chất điện phân x = 0,4, dẫn đến độ dẫn điện tương đối cao mặc dù có nồng độ cao và độ nhớt cao. Các phép đo nanoindentation đối với các lớp phủ Al có độ tinh khiết >99% cho thấy độ cứng nano là 2,86 GPa, gấp ba lần so với các vật liệu Al được tạo ra thông qua quá trình điện cực từ bồn chất lỏng ion nổi tiếng hoặc thông qua biến dạng dẻo nghiêm trọng. Ngoài ra, các lớp phủ Al không có lỗ rỗng có hướng tinh thể ưu tiên <100>, giúp tăng khả năng chống ăn mòn tự do và ăn mòn rỗ. Các cuộc thảo luận về vi cấu trúc nhỏ gọn và hướng tinh thể <100> của các lớp phủ chỉ thu được từ chất điện phân cô đặc x = 0,4 cũng được trình bày."} +{"idx": 1870745, "en": "Diamine-appended variants of the metal-organic framework M2(dobpdc) (M = Mg, Mn, Fe, Co, Zn; dobpdc4- = 4,4'-dioxidobiphenyl-3,3'-dicarboxylate) exhibit exceptional CO2 capture properties owing to a unique cooperative adsorption mechanism, and thus hold promise for use in the development of energy- and cost-efficient CO2 separations. Understanding the nature of thermal transport in these materials is essential for such practical applications, however, as temperature rises resulting from exothermic CO2 uptake could potentially offset the energy savings offered by such cooperative adsorbents. Here, molecular dynamics (MD) simulations are employed in investigating thermal transport in bare and e-2-appended Zn2(dobpdc) (e-2 = N-ethylethylenediamine), both with and without CO2 as a guest. In the absence of CO2, the appended diamines function to enhance thermal conductivity in the ab-plane of e-2-Zn2(dobpdc) relative to the bare framework, as a result of noncovalent interactions between adjacent diamines that provide additional heat transfer pathways across the pore channel. Upon introduction of CO2, the thermal conductivity along the pore channel (the c-axis) increases due to the cooperative formation of metal-bound ammonium carbamates, which serve to create additional heat transfer pathways. In contrast, the thermal conductivity of the bare framework remains unchanged in the presence of zinc-bound CO2 but decreases in the presence of additional adsorbed CO2.", "gemini": "Các biến thể được bổ sung diamine của khung hữu cơ kim loại M2(dobpdc) (M = Mg, Mn, Fe, Co, Zn; dobpdc4- = 4,4'-dioxidobiphenyl-3,3'-dicarboxylate) thể hiện các tính chất hấp phụ CO2 đặc biệt do cơ chế hấp phụ hợp tác độc đáo, và do đó hứa hẹn sẽ được sử dụng trong quá trình phát triển các phương pháp tách CO2 tiết kiệm năng lượng và chi phí. Tuy nhiên, việc hiểu được bản chất của quá trình truyền nhiệt trong các vật liệu này là rất cần thiết cho các ứng dụng thực tế như vậy, vì nhiệt độ tăng do hấp thụ CO2 tỏa nhiệt có khả năng bù đắp lượng năng lượng tiết kiệm được do các chất hấp phụ hợp tác như vậy. Ở đây, các mô phỏng động học phân tử (MD) được sử dụng để nghiên cứu quá trình truyền nhiệt trong Zn2(dobpdc) (e-2 = N-ethylethylenediamine) trần và bổ sung e-2, có và không có CO2 làm chất khách. Khi không có CO2, các diamine được bổ sung có chức năng tăng độ dẫn nhiệt trong mặt phẳng ab của e-2-Zn2(dobpdc) so với khung trần, do các tương tác không cộng hóa trị giữa các diamine kề nhau cung cấp thêm các đường truyền nhiệt trên kênh lỗ chân lông. Khi đưa CO2 vào, độ dẫn nhiệt dọc theo kênh lỗ chân lông (trục c) tăng lên do sự hình thành hợp tác của các carbamat amoni liên kết kim loại, đóng vai trò tạo ra các đường truyền nhiệt bổ sung. Ngược lại, độ dẫn nhiệt của khung trần vẫn không thay đổi khi có CO2 liên kết kẽm nhưng giảm khi có thêm CO2 hấp phụ."} +{"idx": 1870746, "en": "From the granular and fractured subsurface environment to highly engineered polymer membranes used in pharmaceutical purification, porous materials are ubiquitous in nature and industrial applications. In particular, porous media are used extensively in processes including water treatment, pharmaceutical sterilization, food/beverage processing, and heterogeneous catalysis, where hindered mass transport is either essential to the process or a necessary but undesirable limitation. Unfortunately, there are currently no universal models capable of predicting mass transport based on a description of the porous material because real porous materials are complex and because many coupled dynamic mechanisms (e.g., adsorption, steric effects, hydrodynamic effects, electrostatic interactions, etc.) give rise to the observed macroscopic transport phenomena.While classical techniques, like nuclear magnetic resonance and dynamic light scattering, provide useful information about mass transport in porous media at the ensemble level, they provide limited insight into the microscopic mechanisms that give rise to complex phenomena such as anomalous diffusion, hindered pore-space accessibility, and unexpected retention under flow, among many others. To address this issue, we have developed refractive index matching imaging systems, combined with single-particle tracking methods, allowing the direct visualization of single-particle motion within a variety of porous materials.In this Account, we summarize our recent efforts to advance the understanding of nanoparticle transport in porous media using single-particle tracking methods in both fundamental and applied scenarios. First, we describe the basic principles for two-dimensional and three-dimensional single-particle tracking in porous materials. Then, we provide concrete examples of nanoparticle transport in porous materials from two perspectives: (1) understanding fundamental elementary particle transport processes in porous media, including pore accessibility and cavity escape, which limit transport in porous media, and (2) facilitating applications in industrial processes, e.g., by understanding the mechanisms of particle fouling and remobilization in filtration membranes. Finally, we provide an outlook of opportunities associated with investigating other types of mass transport in confined environments using single-particle tracking methods, including electrophoretic and self-propelled motion.", "gemini": "Từ môi trường bề mặt dưới lòng đất dạng hạt và gãy nứt đến màng polyme được chế tạo cao cấp được sử dụng trong quá trình tinh chế dược phẩm, các vật liệu xốp có mặt ở khắp nơi trong tự nhiên và các ứng dụng công nghiệp. Đặc biệt, vật liệu xốp được sử dụng rộng rãi trong các quy trình bao gồm xử lý nước, khử trùng dược phẩm, chế biến thực phẩm/đồ uống và xúc tác không đồng nhất, trong đó quá trình vận chuyển khối lượng bị cản trở là rất cần thiết cho quy trình hoặc là một hạn chế cần thiết nhưng không mong muốn. Thật không may, hiện tại không có mô hình phổ quát nào có khả năng dự đoán quá trình vận chuyển khối lượng dựa trên mô tả về vật liệu xốp vì các vật liệu xốp thực tế rất phức tạp và vì nhiều cơ chế động học liên hợp (ví dụ: hấp phụ, hiệu ứng lập thể, hiệu ứng thủy động lực, tương tác tĩnh điện, v.v.) làm phát sinh các hiện tượng vận chuyển vĩ mô quan sát được. Mặc dù các kỹ thuật cổ điển, như cộng hưởng từ hạt nhân và tán xạ ánh sáng động, cung cấp thông tin hữu ích về quá trình vận chuyển khối lượng trong môi trường xốp ở cấp độ tổng hợp, nhưng chúng cung cấp cái nhìn hạn chế về các cơ chế vi mô làm phát sinh các hiện tượng phức tạp như khuếch tán bất thường, khả năng tiếp cận không gian lỗ chân lông bị cản trở và sự giữ lại bất ngờ dưới dòng chảy, trong số nhiều hiện tượng khác. Để giải quyết vấn đề này, chúng tôi đã phát triển các hệ thống hình ảnh khớp chỉ số khúc xạ, kết hợp với các phương pháp theo dõi từng hạt, cho phép trực quan hóa trực tiếp chuyển động của từng hạt trong nhiều loại vật liệu xốp. Trong Tài khoản này, chúng tôi tóm tắt những nỗ lực gần đây của mình nhằm thúc đẩy sự hiểu biết về quá trình vận chuyển hạt nano trong môi trường xốp bằng cách sử dụng các phương pháp theo dõi từng hạt trong cả các tình huống cơ bản và ứng dụng. Đầu tiên, chúng tôi mô tả các nguyên tắc cơ bản để theo dõi từng hạt hai chiều và ba chiều trong các vật liệu xốp. Sau đó, chúng tôi đưa ra các ví dụ cụ thể về quá trình vận chuyển hạt nano trong các vật liệu xốp từ hai góc độ: (1) hiểu các quá trình vận chuyển hạt cơ bản cơ bản trong môi trường xốp, bao gồm khả năng tiếp cận lỗ chân lông và thoát khoang, hạn chế quá trình vận chuyển trong môi trường xốp và (2) tạo điều kiện cho các ứng dụng trong các quy trình công nghiệp, ví dụ như bằng cách hiểu các cơ chế bám bẩn hạt và tái vận chuyển trong màng lọc. Cuối cùng, chúng tôi đưa ra triển vọng về các cơ hội liên quan đến việc nghiên cứu các loại vận chuyển khối lượng khác trong môi trường hạn chế bằng cách sử dụng các phương pháp theo dõi từng hạt, bao gồm chuyển động điện di và tự đẩy."} +{"idx": 1870747, "en": "A significant proportion of people who test positive for COVID-19 have chemosensory deficits. However, the reported prevalence of these deficits in smell and taste varies widely, and the reason for the differences between studies is unclear. We determined the pooled prevalence of such chemosensory deficits in a systematic review and meta-analysis. We searched the COVID-19 portfolio of the National Institutes of Health for studies that reported the prevalence of smell or taste deficits or both in patients diagnosed with COVID-19. One-hundred-four studies reporting on 38 198 patients qualified and were subjected to a systematic review and meta-analysis. Estimated random prevalence of olfactory dysfunction was 43.0%, that of taste dysfunction was 44.6%, and that of overall chemosensory dysfunction was 47.4%. We examined the effects of age, gender, disease severity, and ethnicity on chemosensory dysfunction. Prevalence of smell or taste dysfunction or both decreased with older age, male gender, and disease severity. Ethnicity was highly significant: Caucasians had a three times higher prevalence of chemosensory dysfunctions (54.8%) than Asians (17.7%). The finding of geographic differences points to two causes that are not mutually exclusive. A virus mutation (D614G) may cause differing infectivity, while at the host level genetic, ethnicity-specific variants of the virus-binding entry proteins may facilitate virus entry in the olfactory epithelium and taste buds. Both explanations have major implications for infectivity, diagnosis, and management of the COVID-19 pandemic.", "gemini": "Một tỷ lệ đáng kể những người có kết quả xét nghiệm dương tính với COVID-19 bị thiếu hụt về cảm giác hóa học. Tuy nhiên, tỷ lệ mắc các khiếm khuyết về khứu giác và vị giác được báo cáo có sự khác biệt rất lớn và lý do cho sự khác biệt giữa các nghiên cứu vẫn chưa rõ ràng. Chúng tôi đã xác định tỷ lệ mắc chung của những khiếm khuyết về cảm giác hóa học như vậy trong một đánh giá có hệ thống và phân tích tổng hợp. Chúng tôi đã tìm kiếm danh mục đầu tư COVID-19 của Viện Y tế Quốc gia để tìm các nghiên cứu báo cáo tỷ lệ mắc các khiếm khuyết về khứu giác hoặc vị giác hoặc cả hai ở những bệnh nhân được chẩn đoán mắc COVID-19. Một trăm bốn nghiên cứu báo cáo về 38.198 bệnh nhân đủ điều kiện và được đưa vào đánh giá có hệ thống và phân tích tổng hợp. Ước tính tỷ lệ mắc ngẫu nhiên của rối loạn chức năng khứu giác là 43,0%, rối loạn chức năng vị giác là 44,6% và rối loạn chức năng cảm giác hóa học nói chung là 47,4%. Chúng tôi đã kiểm tra ảnh hưởng của tuổi tác, giới tính, mức độ nghiêm trọng của bệnh và dân tộc đối với rối loạn chức năng cảm giác hóa học. Tỷ lệ mắc rối loạn chức năng khứu giác hoặc vị giác hoặc cả hai đều giảm theo độ tuổi cao hơn, nam giới và mức độ nghiêm trọng của bệnh. Dân tộc có ý nghĩa rất lớn: Người da trắng có tỷ lệ mắc các rối loạn chức năng cảm giác hóa học cao gấp ba lần (54,8%) so với người châu Á (17,7%). Phát hiện về sự khác biệt về mặt địa lý chỉ ra hai nguyên nhân không loại trừ lẫn nhau. Một đột biến của vi-rút (D614G) có thể gây ra khả năng lây nhiễm khác nhau, trong khi ở cấp độ vật chủ, các biến thể đặc hiệu về mặt dân tộc của các protein gắn vào vi-rút có thể tạo điều kiện cho vi-rút xâm nhập vào biểu mô khứu giác và nụ vị giác. Cả hai lời giải thích đều có những tác động lớn đến khả năng lây nhiễm, chẩn đoán và quản lý đại dịch COVID-19."} +{"idx": 1870748, "en": "Exposure to excessive ultraviolet (UV) radiation can have detrimental effects on human health. Inexpensive easy-to-use sensors for monitoring UV radiation can allow broad-scale assessment of UV exposure, but their implementation requires technology that enables rapid and affordable manufacturing of these sensors on a large scale. Herein, we report a novel three-dimensional (3D) printing procedure and printable ink composition that produce robust, flexible, and wearable UV sensors. To fabricate the sensors, a color-changing hydrogel ink was first developed from which standalone constructs were 3D printed. The ink contains alginate, gelatin, photoactive titanium dioxide nanoparticles, and dyes (methyl orange, methylene blue, and malachite green) in which the nanoparticles are used to initiate photocatalytic degradation of dyes, leading to discoloration of the dye. The sensors resemble a color-changing tattoo that loses color upon exposure to UV. The viscosity and ink composition were optimized to achieve printability and tune the mechanical properties (e.g., modulus, hardness) of the sensors. The optimized procedure enabled the one-step fabrication of mechanically stable sensors that can effectively measure outdoor sun exposure by quantifying the decrease in color, visible to the naked eye. Apart from being used as wearable sensors, these sensors have the potential to be used along with UV-based workspace sterilizing devices to ensure that surfaces have been efficiently exposed to UV. The sensors are inexpensive, stable, extremely robust, biodegradable, and easy to use. The tunability, biocompatibility, and printability of the ink offer excellent potential for developing advanced 3D printing methods that, in addition to UV sensors, can be applied more broadly to fabricate other sensing technologies for a variety of other applications.", "gemini": "Tiếp xúc với bức xạ cực tím (UV) quá mức có thể gây ra những ảnh hưởng có hại cho sức khỏe con người. Các cảm biến giá rẻ, dễ sử dụng để theo dõi bức xạ UV có thể cho phép đánh giá trên diện rộng về mức độ tiếp xúc với UV, nhưng việc triển khai chúng đòi hỏi công nghệ cho phép sản xuất nhanh chóng và giá cả phải chăng các cảm biến này trên quy mô lớn. Trong bài báo này, chúng tôi báo cáo một quy trình in ba chiều (3D) mới và thành phần mực có thể in được tạo ra các cảm biến UV chắc chắn, linh hoạt và có thể đeo được. Để chế tạo các cảm biến, đầu tiên, một loại mực hydrogel đổi màu đã được phát triển, từ đó các cấu trúc độc lập được in 3D. Mực chứa alginate, gelatin, các hạt nano titanium dioxide hoạt động quang và thuốc nhuộm (methyl cam, xanh methylen và xanh malachite), trong đó các hạt nano được sử dụng để khởi động quá trình phân hủy quang xúc tác các thuốc nhuộm, dẫn đến sự đổi màu của thuốc nhuộm. Các cảm biến giống như một hình xăm đổi màu mất màu khi tiếp xúc với tia UV. Độ nhớt và thành phần mực đã được tối ưu hóa để đạt được khả năng in và điều chỉnh các tính chất cơ học (ví dụ: mô đun, độ cứng) của các cảm biến. Quy trình được tối ưu hóa cho phép chế tạo một bước các cảm biến ổn định về mặt cơ học có thể đo hiệu quả mức độ tiếp xúc với ánh nắng mặt trời ngoài trời bằng cách định lượng sự giảm màu, có thể nhìn thấy bằng mắt thường. Ngoài việc được sử dụng làm cảm biến đeo được, các cảm biến này có khả năng được sử dụng cùng với các thiết bị khử trùng không gian làm việc dựa trên tia UV để đảm bảo rằng các bề mặt đã được tiếp xúc hiệu quả với tia UV. Các cảm biến không tốn kém, ổn định, cực kỳ chắc chắn, có thể phân hủy sinh học và dễ sử dụng. Khả năng điều chỉnh, tương thích sinh học và khả năng in của mực mang lại tiềm năng tuyệt vời để phát triển các phương pháp in 3D tiên tiến, ngoài cảm biến UV, có thể được áp dụng rộng rãi hơn để chế tạo các công nghệ cảm biến khác cho nhiều ứng dụng khác nhau."} +{"idx": 1870749, "en": "Heterojunctioned transition-metal dichalcogenide (TMD) films with regulatable interface adhesion have shown broad application prospects in the design of advanced materials and the manufacturing of novel functional devices. To date, the controlled fabrication of TMD heterojunctions or heterojunction-rich films with tailorable thickness and composition has proved challenging. Herein, a bimetallic co-feeding atomic layer deposition (ALD) system was developed capable of fulfilling these requirements. In the co-feeding ALD fabrication, by adjusting the Re/Mo ratio, 3-layered quasibinary heterojunctioned ReS2/MoS2 films with adjustable composition and grain size were prepared. Moreover, the measurements between atomic force microscopy Si tip coated with the ReS2/MoS2 films and films on the substrate indicate that the adhesion force can be regulated from 13.5 to 136.3 nN. Further experimental data and theoretical analysis show that the adhesion force between the coated tip and films possesses a positive correlation with the \"tip-film unanimity\" in composition.", "gemini": "Các màng dichalcogenide kim loại chuyển tiếp (TMD) dị hợp với độ bám dính giao diện có thể điều chỉnh đã cho thấy triển vọng ứng dụng rộng rãi trong thiết kế vật liệu tiên tiến và sản xuất các thiết bị chức năng mới. Cho đến nay, việc chế tạo có kiểm soát các dị hợp chất TMD hoặc màng giàu dị hợp chất với độ dày và thành phần có thể điều chỉnh đã được chứng minh là một thách thức. Trong bài báo này, một hệ thống lắng đọng lớp nguyên tử (ALD) đồng cấp liệu kim loại kép đã được phát triển có khả năng đáp ứng các yêu cầu này. Trong chế tạo ALD đồng cấp liệu, bằng cách điều chỉnh tỷ lệ Re/Mo, các màng ReS2/MoS2 dị hợp bán nhị phân 3 lớp với thành phần và kích thước hạt có thể điều chỉnh đã được chế tạo. Hơn nữa, các phép đo giữa đầu dò Si của kính hiển vi lực nguyên tử được phủ màng ReS2/MoS2 và màng trên giá thể chỉ ra rằng lực bám dính có thể được điều chỉnh từ 13,5 đến 136,3 nN. Dữ liệu thực nghiệm và phân tích lý thuyết cho thấy lực bám dính giữa đầu dò phủ và màng có mối tương quan tích cực với \"sự nhất trí đầu dò-màng\" về thành phần."} +{"idx": 1870750, "en": "Developing a simple and universal solution for gripping fragile, multiscaled, and arbitrary-shaped objects using a robot gripper is challenging. Herein, we propose a universal, shape-adaptive/-retaining and reversible, hardness-variable gripper skin that serves as a resourceful solution for grasping such objects without damaging them. The proposed universal gripper skin based on a magnetorheological elastomer is attached to a robot gripper. The proposed skin takes the shape of a target object as soon as the gripper grasps the object. At this time, we solidify the gripper skin by applying a magnetic field, thereby allowing the gripper to grasp the target object easily. After releasing the objects, the magnetic field is removed and the deformed proposed gripper skin rapidly restores its original shape. The proposed adaptive gripper skin is made to grasp various target objects, such as cylinders, cuboids, and triangular prisms, based on which its grasping performance is evaluated.", "gemini": "Việc phát triển một giải pháp đơn giản và phổ quát để kẹp các vật thể dễ vỡ, đa tỷ lệ và có hình dạng tùy ý bằng cách sử dụng kẹp robot là một thách thức. Trong bài báo này, chúng tôi đề xuất một lớp da kẹp cứng có thể thay đổi hình dạng/duy trì hình dạng và có thể đảo ngược, có thể phục vụ như một giải pháp hữu ích để kẹp các vật thể như vậy mà không làm hỏng chúng. Lớp da kẹp phổ quát được đề xuất dựa trên chất đàn hồi từ lưu biến được gắn vào kẹp robot. Lớp da được đề xuất sẽ có hình dạng của vật thể mục tiêu ngay khi kẹp kẹp vật thể. Lúc này, chúng tôi làm đông cứng lớp da kẹp bằng cách áp dụng từ trường, do đó cho phép kẹp dễ dàng kẹp vật thể mục tiêu. Sau khi nhả các vật thể, từ trường sẽ bị loại bỏ và lớp da kẹp được đề xuất bị biến dạng sẽ nhanh chóng phục hồi hình dạng ban đầu của nó. Lớp da kẹp thích ứng được đề xuất được tạo ra để kẹp các vật thể mục tiêu khác nhau, chẳng hạn như hình trụ, hình hộp chữ nhật và lăng trụ tam giác, dựa trên đó hiệu suất kẹp của nó được đánh giá."} +{"idx": 1870751, "en": "Zn(O,S) has been successfully doped with different amounts of Ho and characterized with X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), diffuse reflectance spectroscopy (DRS), X-ray photoelectron spectroscopy (XPS), electrochemical impedance spectroscopy (EIS), and transient photocurrent (TPC). The as-prepared Ho-doped Zn(O,S) catalysts with different Ho amounts are evaluated for hydrogen evolution reaction. The catalyst with the best performance in evolving hydrogen is further utilized to hydrogenate 4-nitrophenol (4-NP) to 4-aminophenol (4-AP). It is found that doping with Ho obviously enhanced charge transfer and photoresponse properties of the catalyst. Therefore, the modified Zn(O,S) can optimally evolve hydrogen by 18 624 μmol/g, which is 20% higher than that of pristine Zn(O,S). Subsequently, the in situ generated hydrogen ions on catalyst surfaces also play an important role as a hydrogen source to hydrogenate 4-NP to 4-AP without any reducing agents such as NaBH4, which is commonly used as a hydrogen source. As Ho is doped in the lattices of Zn(O,S), it acts not only to separate photocarriers and to enhance the charge transfer but also to shorten the diffusion time of nitrophenolate ions to catalyst surfaces for further photocatalytic hydrogenation reaction (PHR) process. A plausible PHR mechanism has been provided to elucidate the great performance of Ho-modified Zn(O,S) for photocatalytic hydrogenation.", "gemini": "Zn(O,S) đã được pha tạp thành công với các lượng Ho khác nhau và được đặc trưng bằng nhiễu xạ tia X (XRD), kính hiển vi điện tử quét (SEM), kính hiển vi điện tử truyền qua (TEM), quang phổ phản xạ khuếch tán (DRS), quang phổ điện tử tia X (XPS), điện hóa trở kháng quang phổ (EIS) và quang điện tử thoáng qua (TPC). Các chất xúc tác Zn(O,S) pha tạp Ho được chế tạo với các lượng Ho khác nhau được đánh giá cho phản ứng tiến hóa hydro. Chất xúc tác có hiệu suất tốt nhất trong quá trình tiến hóa hydro được sử dụng để hydro hóa 4-nitrophenol (4-NP) thành 4-aminophenol (4-AP). Người ta thấy rằng việc pha tạp với Ho rõ ràng làm tăng cường khả năng truyền điện tích và các đặc tính quang phản ứng của chất xúc tác. Do đó, Zn(O,S) đã được sửa đổi có thể tiến hóa tối ưu hydro ở mức 18.624 μmol/g, cao hơn 20% so với Zn(O,S) nguyên chất. Sau đó, các ion hydro được tạo ra tại chỗ trên bề mặt chất xúc tác cũng đóng vai trò quan trọng như một nguồn hydro để hydro hóa 4-NP thành 4-AP mà không cần bất kỳ chất khử nào như NaBH4, thường được sử dụng làm nguồn hydro. Khi Ho được đưa vào mạng tinh thể của Zn(O,S), nó không chỉ có tác dụng tách các hạt mang điện và tăng cường truyền điện tích mà còn rút ngắn thời gian khuếch tán của các ion nitrophenolat đến bề mặt chất xúc tác để phản ứng hydro hóa quang xúc tác (PHR) tiếp theo. Một cơ chế PHR hợp lý đã được đưa ra để làm sáng tỏ hiệu suất tuyệt vời của Zn(O,S) được sửa đổi bằng Ho đối với hydro hóa quang xúc tác."} +{"idx": 1870752, "en": "Marburg virus (MARV) causes sporadic outbreaks of severe disease with high case fatality rates in humans. To date, neither therapeutics nor prophylactic approaches have been approved for MARV disease. The MARV matrix protein VP40 (mVP40) plays central roles in virus assembly and budding. mVP40 also inhibits interferon signaling by inhibiting the function of Janus kinase 1. This suppression of host antiviral defenses likely contributes to MARV virulence and therefore is a potential therapeutic target. We developed and optimized a cell-based high-throughput screening (HTS) assay in 384-well format to measure mVP40 interferon (IFN) antagonist function such that inhibitors could be identified. We performed a pilot screen of 1280 bioactive compounds and identified 3 hits, azaguanine-8, tosufloxacin hydrochloride, and linezolid, with Z scores > 3 and no significant cytotoxicity. Of these, azaguanine-8 inhibited MARV growth at noncytotoxic concentrations. These data demonstrate the suitability of the HTS mVP40 assay for drug discovery and suggest potential directions for anti-MARV therapeutic development.", "gemini": "Virus Marburg (MARV) gây ra các đợt bùng phát lẻ tẻ của bệnh nghiêm trọng với tỷ lệ tử vong cao ở người. Cho đến nay, vẫn chưa có phương pháp điều trị hoặc biện pháp phòng ngừa nào được chấp thuận cho bệnh MARV. Protein ma trận MARV VP40 (mVP40) đóng vai trò trung tâm trong quá trình lắp ráp và nảy chồi của vi-rút. mVP40 cũng ức chế tín hiệu interferon bằng cách ức chế chức năng của Janus kinase 1. Sự ức chế các cơ chế bảo vệ chống vi-rút của vật chủ này có khả năng góp phần vào độc lực của MARV và do đó là mục tiêu điều trị tiềm năng. Chúng tôi đã phát triển và tối ưu hóa một xét nghiệm sàng lọc hiệu suất cao (HTS) dựa trên tế bào ở định dạng 384 giếng để đo chức năng đối kháng interferon (IFN) của mVP40 để có thể xác định các chất ức chế. Chúng tôi đã thực hiện một sàng lọc thí điểm gồm 1280 hợp chất hoạt tính sinh học và xác định được 3 hợp chất trúng đích, azaguanine-8, tosufloxacin hydrochloride và linezolid, với điểm Z > 3 và không có độc tính tế bào đáng kể. Trong số các hợp chất này, azaguanine-8 ức chế sự phát triển của MARV ở nồng độ không gây độc tế bào. Các dữ liệu này chứng minh tính phù hợp của xét nghiệm mVP40 HTS để phát hiện thuốc và gợi ý các hướng tiềm năng cho quá trình phát triển phương pháp điều trị chống MARV."} +{"idx": 1870753, "en": "Flexible thermoelectric devices can be potentially used for flexible cooling and energy harvesting from various heat sources such as the human body. However, the development of flexible thermoelectric materials with excellent thermoelectric performance is still very challenging. In this study, a simple solution process is proposed for the preparation of flexible inorganic/carbon nanotube hybrid films with record power factors among those of the reported flexible n-type thermoelectric materials. The hybrid films fabricated by bar-coating a carbon nanotube-dispersed Sb2Te3 solution exhibit n-type power factors of up to 2440 ± 267 μV m-1 K-2 at room temperature. The dissolved Sb2Te3 recrystallizes on the carbon nanotube surfaces and form hybrid solids. The ultrahigh power factor may be originated from the effective n-doping of carbon nanotubes by the oxidation of neighboring Sb2Te3. Using the thermoelectric hybrid film, a multilayer stacked thermoelectric generator is fabricated. The flexible device with a thermal contact area of 3.8 cm2 exhibits an output power of up to 11.3 μW at a vertical ΔT of 7.5 K. This study paves the way for the realization of flexible thermoelectric devices with various device geometries.", "gemini": "Các thiết bị nhiệt điện linh hoạt có thể được sử dụng để làm mát linh hoạt và thu năng lượng từ nhiều nguồn nhiệt khác nhau như cơ thể con người. Tuy nhiên, việc phát triển các vật liệu nhiệt điện linh hoạt có hiệu suất nhiệt điện tuyệt vời vẫn còn rất nhiều thách thức. Trong nghiên cứu này, một quy trình dung dịch đơn giản được đề xuất để chế tạo các màng lai ống nano vô cơ/carbon linh hoạt với các hệ số công suất kỷ lục trong số các vật liệu nhiệt điện loại n linh hoạt đã được báo cáo. Các màng lai được chế tạo bằng cách tráng thanh một dung dịch Sb2Te3 phân tán ống nano carbon thể hiện các hệ số công suất loại n lên đến 2440 ± 267 μV m-1 K-2 ở nhiệt độ phòng. Sb2Te3 hòa tan kết tinh lại trên bề mặt ống nano carbon và tạo thành chất rắn lai. Hệ số công suất cực cao có thể có nguồn gốc từ việc n-doping hiệu quả của ống nano carbon do quá trình oxy hóa Sb2Te3 lân cận. Sử dụng màng lai nhiệt điện, một máy phát nhiệt điện nhiều lớp được chế tạo. Thiết bị linh hoạt có diện tích tiếp xúc nhiệt là 3,8 cm2 thể hiện công suất đầu ra lên đến 11,3 μW ở ΔT thẳng đứng là 7,5 K. Nghiên cứu này mở đường cho việc chế tạo các thiết bị nhiệt điện linh hoạt với nhiều hình dạng thiết bị khác nhau."} +{"idx": 1870754, "en": "Herein, we propose the use of novel adsorbents, namely micelle clay complexes comprising the clay montmorillonite, coupled with activated carbon for effective eradication of neuropathogenic microbes such as SARS-CoV-2 and Naegleria fowleri from water supplies for ablution/nasal irrigation. These can be incorporated easily to water collection devices, i.e., taps and water bottles, in the domestic setting. These filters are low cost, easy to install, and ideal disinfection systems. Such strategies are particularly useful for communities who have lack of access to safe water supplies, rely heavily on water storage tanks, or lack adequate water sanitation facilities, especially in developing countries.", "gemini": "Trong bài báo này, chúng tôi đề xuất sử dụng các chất hấp phụ mới, cụ thể là các phức hợp đất sét micelle bao gồm đất sét montmorillonite, kết hợp với than hoạt tính để diệt trừ hiệu quả các vi khuẩn gây bệnh thần kinh như SARS-CoV-2 và Naegleria fowleri khỏi nguồn cung cấp nước để rửa/tưới mũi. Những chất này có thể dễ dàng kết hợp vào các thiết bị lấy nước, tức là vòi nước và bình nước, trong môi trường gia đình. Các bộ lọc này có chi phí thấp, dễ lắp đặt và là hệ thống khử trùng lý tưởng. Các chiến lược như vậy đặc biệt hữu ích cho các cộng đồng không có nguồn cung cấp nước an toàn, phụ thuộc nhiều vào bể chứa nước hoặc thiếu các cơ sở vệ sinh nước đầy đủ, đặc biệt là ở các nước đang phát triển."} +{"idx": 1870755, "en": "The aggregation of neurotoxic amyloid-β (Aβ) polypeptides into aberrant extracellular senile plaques is the major neuropathological hallmark of Alzheimer's disease (AD). Inhibiting aggregation of these peptides to control the progression of this deadly disease can serve as a viable therapeutic option. In the current work, inherently fluorescent theranostic dopamine-tryptophan nanocomposites (DTNPs) were developed and investigated for their amyloid inhibition propensity along with their ability to act as a cellular bioimaging agent in neuronal cells. The antiaggregation potency of the nanocomposites was further investigated against an in vitro established reductionist amyloid aggregation model consisting of a mere dipeptide, phenylalanine-phenylalanine (FF). As opposed to large peptide/protein-derived robust and high-molecular-weight amyloid aggregation models of Alzheimer's disease, our dipeptide-based amyloid model provides an edge over others in terms of the ease of handling, synthesis, and cost-effectiveness. Results demonstrated positive antiaggregation behavior of the DTNPs toward both FF-derived amyloid fibrils and preformed Aβ-peptide fibers by means of electron microscopic and circular dichroism-based studies. Our results further pointed toward the neuroprotective effects of the DTNPs in neuroblastoma cells against FF amyloid fibril-induced toxicity and also that they significantly suppressed the accumulation of Aβ42 oligomers in both cortex and hippocampus regions and improved cognitive impairment in an intracerebroventricular streptozotocin (ICV-STZ)-induced animal model of dementia. Besides, DTNPs also exhibited excellent fluorescent properties and light up the cytoplasm of neuroblastoma cells when being coincubated with cells, confirming their ability to serve as an intracellular bioimaging agent. Overall, these results signify the potency of the DTNPs as promising multifunctional theranostic agents for treating AD.", "gemini": "Sự kết tập của các polypeptide amyloid-β (Aβ) gây độc cho thần kinh thành các mảng senile ngoại bào bất thường là dấu hiệu bệnh lý thần kinh chính của bệnh Alzheimer (AD). Ức chế sự kết tập của các peptide này để kiểm soát sự tiến triển của căn bệnh chết người này có thể đóng vai trò là một lựa chọn điều trị khả thi. Trong công trình hiện tại, các nanocomposite dopamine-tryptophan (DTNP) chẩn đoán và điều trị có khả năng huỳnh quang vốn có đã được phát triển và nghiên cứu về khả năng ức chế amyloid của chúng cùng với khả năng hoạt động như một tác nhân tạo ảnh sinh học tế bào trong các tế bào thần kinh. Khả năng chống kết tập của các nanocomposite đã được nghiên cứu sâu hơn đối với một mô hình kết tập amyloid giản lược đã thiết lập trong ống nghiệm bao gồm một dipeptide đơn thuần, phenylalanine-phenylalanine (FF). Trái ngược với các mô hình kết tập amyloid mạnh và có trọng lượng phân tử cao có nguồn gốc từ peptide/protein lớn của bệnh Alzheimer, mô hình amyloid dựa trên dipeptide của chúng tôi cung cấp một lợi thế hơn những mô hình khác về tính dễ xử lý, tổng hợp và hiệu quả về chi phí. Kết quả chứng minh hành vi chống kết tập tích cực của DTNP đối với cả các sợi fibril amyloid có nguồn gốc từ FF và các sợi peptide Aβ đã tạo thành thông qua các nghiên cứu dựa trên kính hiển vi điện tử và độ sắc quang tròn. Kết quả của chúng tôi chỉ ra thêm các tác động bảo vệ thần kinh của DTNP trong các tế bào nguyên bào thần kinh đối với độc tính do sợi fibril amyloid FF gây ra và chúng cũng ức chế đáng kể sự tích tụ của các oligome Aβ42 ở cả vùng vỏ não và vùng đồi hải mã và cải thiện suy giảm nhận thức trong một mô hình động vật mắc chứng mất trí nhớ do streptozotocin trong não thất (ICV-STZ). Bên cạnh đó, DTNP cũng biểu hiện các tính chất huỳnh quang tuyệt vời và làm sáng tế bào chất của các tế bào nguyên bào thần kinh khi được đồng ủ với các tế bào, xác nhận khả năng hoạt động như một tác nhân tạo ảnh sinh học trong tế bào của chúng. Nhìn chung, những kết quả này biểu thị tiềm năng của DTNP như các tác nhân chẩn đoán và điều trị đa chức năng đầy hứa hẹn để điều trị AD."} +{"idx": 1870756, "en": "In line with the classic phonon-glass electron-crystal (PGEC) paradigm, semiconducting and semimetallic multinary compounds remain the cornerstone of the state-of-the-art thermoelectric materials. By contrast, elemental PGEC is very rare. In this work, we report a thermoelectric study of monolayer α-Te by first-principles calculations and solving the parameter-free Boltzmann transport equation. It is found that monolayer α-Te possesses high electron mobility (about 2500 cm2 V-1 s-1) at room temperature due to small effective mass, low phonon frequencies, and thus a restricted phase space for electron-phonon scattering. In monolayer α-Te, the electrons near the conduction band edge are mainly scattered by the heavily populated quadratically dispersing out-of-plane acoustic (ZA) phonon modes. The thermoelectric figure of merit (ZT) for n-type monolayer α-Te is 0.55 at 300 K and 1.46 at 700 K. Notably, tensile strain stiffens the ZA modes, yielding a linear energy-momentum dispersion relation and the removal of the diverging thermal population of ZA phonons. Consequently, the electron mobility is enhanced. At a 4% tensile strain, the electron mobility can reach up to 8000 cm2 V-1 s-1 at room temperature while the thermal conductivity is almost unaffected, yielding a state-of-the-art ZT value of 0.94 and 2.03 in n-type monolayer α-Te at 300 and 700 K, respectively. For completeness, the thermoelectric study of p-type monolayer α-Te is also conducted. These results beckon further experiments toward high-performance α-Te-based thermoelectric materials via doping, alloying, and compositing.", "gemini": "Phù hợp với mô hình phonon-thủy tinh electron-tinh thể (PGEC) cổ điển, các hợp chất đa nguyên bán dẫn và bán kim loại vẫn là nền tảng của vật liệu nhiệt điện hiện đại. Ngược lại, PGEC nguyên tố rất hiếm. Trong nghiên cứu này, chúng tôi báo cáo một nghiên cứu nhiệt điện về lớp đơn α-Te bằng các phép tính nguyên lý đầu tiên và giải phương trình vận chuyển Boltzmann không có tham số. Người ta thấy rằng lớp đơn α-Te sở hữu độ linh động điện tử cao (khoảng 2500 cm2 V-1 s-1) ở nhiệt độ phòng do khối lượng hiệu dụng nhỏ, tần số phonon thấp và do đó, không gian pha bị hạn chế cho sự tán xạ electron-phonon. Trong lớp đơn α-Te, các electron gần mép dải dẫn chủ yếu bị tán xạ bởi các chế độ phonon âm học ngoài mặt phẳng (ZA) phân tán theo bậc hai có mật độ cao. Hệ số hiệu suất nhiệt điện (ZT) đối với lớp đơn α-Te loại n là 0,55 ở 300 K và 1,46 ở 700 K. Đáng chú ý, ứng suất kéo làm cứng các chế độ ZA, tạo ra mối quan hệ phân tán năng lượng-động lượng tuyến tính và loại bỏ mật độ nhiệt phân kỳ của phonon ZA. Do đó, độ linh động của electron được tăng cường. Ở ứng suất kéo 4%, độ linh động của electron có thể đạt tới 8000 cm2 V-1 s-1 ở nhiệt độ phòng trong khi độ dẫn nhiệt hầu như không bị ảnh hưởng, tạo ra giá trị ZT hiện đại là 0,94 và 2,03 trong lớp đơn α-Te loại n ở 300 và 700 K, tương ứng. Để hoàn thiện, nghiên cứu nhiệt điện về lớp đơn α-Te loại p cũng được tiến hành. Những kết quả này báo hiệu thêm các thí nghiệm hướng tới vật liệu nhiệt điện dựa trên α-Te hiệu suất cao thông qua việc pha tạp, hợp kim và ghép."} +{"idx": 1870757, "en": "Two-dimensional (2D) MXene has shown enormous potential in scientific fields, including energy storage and electromagnetic interference (EMI) shielding. Unfortunately, MXene-based material structures generally suffer from mechanical fragility and vulnerability to oxidation. Herein, mussel-inspired dopamine successfully addresses those weaknesses by improving interflake interaction and ordering in MXene assembled films. Dopamine undergoes in situ polymerization and binding at MXene flake surfaces by spontaneous interfacial charge transfer, yielding an ultrathin adhesive layer. Resultant nanocomposites with highly aligned tight layer structures achieve approximately seven times enhanced tensile strength with a simultaneous increase of elongation. Ambient stability of MXene films is also greatly improved by the effective screening of oxygen and moisture. Interestingly, angstrom thick polydopamine further promotes the innate high electrical conductivity and excellent EMI shielding properties of MXene films. This synergistic concurrent enhancement of physical properties proposes MXene/polydopamine hybrids as a general platform for MXene based reliable applications.", "gemini": "MXene hai chiều (2D) đã thể hiện tiềm năng to lớn trong các lĩnh vực khoa học, bao gồm cả lưu trữ năng lượng và che chắn nhiễu điện từ (EMI). Thật không may, các cấu trúc vật liệu dựa trên MXene thường dễ bị gãy cơ học và dễ bị oxy hóa. Trong bài báo này, dopamine lấy cảm hứng từ loài trai đã giải quyết thành công những điểm yếu đó bằng cách cải thiện tương tác giữa các vảy và sắp xếp trong các màng lắp ráp MXene. Dopamine trải qua quá trình trùng hợp tại chỗ và liên kết tại bề mặt vảy MXene bằng cách truyền điện tích giao diện tự phát, tạo ra một lớp keo siêu mỏng. Các nanocomposite kết quả với các cấu trúc lớp chặt chẽ được căn chỉnh cao đạt được độ bền kéo tăng khoảng bảy lần với độ giãn dài tăng đồng thời. Độ ổn định trong môi trường xung quanh của màng MXene cũng được cải thiện đáng kể nhờ khả năng che chắn hiệu quả oxy và độ ẩm. Điều thú vị là polydopamine dày angstrom thúc đẩy hơn nữa độ dẫn điện cao vốn có và các đặc tính che chắn EMI tuyệt vời của màng MXene. Sự tăng cường đồng thời có tính hiệp đồng này của các tính chất vật lý đề xuất các vật liệu lai MXene/polydopamine như một nền tảng chung cho các ứng dụng đáng tin cậy dựa trên MXene."} +{"idx": 1870758, "en": "Sterically demanding secondary potassium phosphides (4) were synthesized and investigated. Reaction with halophosphanes (5) yields diphosphanes (6), whereas reaction with CS2 yields phosphanyl dithioformates (10). These can be further converted to the corresponding phosphanyl esters of dithioformic acid R2P-C(S)S-PR2 (8). One of these thioesters (8) was found to undergo a migration reaction, resulting in the formation of a phosphanylthioketone with an additional phosphanylthiolate group (9), which was used as a chiral ligand in gold coordination chemistry. The phosphanyl migration reaction was investigated by spectroscopic and theoretical methods, revealing a first-order reaction via a cyclic transition state. All species mentioned were fully characterized.", "gemini": "Các phosphua kali bậc hai có cấu trúc lập thể (4) đã được tổng hợp và nghiên cứu. Phản ứng với halophosphane (5) tạo ra diphosphane (6), trong khi phản ứng với CS2 tạo ra phosphanyl dithioformat (10). Những chất này có thể được chuyển đổi thêm thành các este phosphanyl tương ứng của axit dithioformic R2P-C(S)S-PR2 (8). Một trong những thioeste này (8) được phát hiện trải qua phản ứng di chuyển, dẫn đến sự hình thành của phosphanylthioketone với một nhóm phosphanylthiolate bổ sung (9), được sử dụng làm phối tử không đối xứng trong hóa học phối hợp vàng. Phản ứng di chuyển phosphanyl đã được nghiên cứu bằng các phương pháp quang phổ và lý thuyết, cho thấy phản ứng bậc nhất thông qua trạng thái chuyển tiếp vòng. Tất cả các loài được đề cập đều được mô tả đầy đủ."} +{"idx": 1870759, "en": "Self-assembling natural small molecules (NSMs) with favorable anticancer activity are of increasing interest as novel drug delivery platforms without structural modification for biomedical applications. However, a lack of knowledge and practicability of NSMs as drug carriers limited their current biomedical application. Here, via a green and facile supramolecular coassembly strategy, we report and develop a series of carrier-free terpenoid natural small molecule-mediated coassembled photosensitive drugs for enhanced and synergistic chemo/photodynamic therapy. After screening 17 terpenoid NSMs, we identified 11 compounds that could form coassembled NSMs-Ce6 NPs with regulatable drug sizes. Analysis of the representative betulonic acid (BC)-mediated nano-coassemblies (BC-Ce6 NPs) reveals the high efficiency of the coassembly strategy and highlights the tremendous potential of NSMs as novel drug delivery platforms. Through molecular dynamics simulation and theoretical calculations, we elucidate the mystery of the coassembly process, indicating that the linear coplanar arrangement of BC dimeric units is primarily responsible for the formation of rod-like or spherical morphology. Meanwhile, we demonstrated that the reduced energy gap between the singlet and triplet excited states (ΔEST) facilitates efficient reactive oxygen species generation by promoting ·OH generation via a type I photoreaction mechanism. The assembled nanodrugs exhibit multiple favorable therapeutic features, ensuring a remarkably enhanced, synergistic, and secure combinatorial anticancer efficacy of 93.6% with highly efficient tumor ablation. This work not only expands the possibility of natural biodegradable materials for wide biological applications but also provides a new perspective for the construction of NSM-mediated nano-coassemblies for precision therapy.", "gemini": "Các phân tử nhỏ tự nhiên tự lắp ráp (NSM) với hoạt tính chống ung thư thuận lợi đang ngày càng được quan tâm như các nền tảng phân phối thuốc mới mà không cần sửa đổi cấu trúc cho các ứng dụng y sinh. Tuy nhiên, việc thiếu kiến thức và tính thực tiễn của NSM như là chất mang thuốc đã hạn chế ứng dụng y sinh hiện tại của chúng. Ở đây, thông qua một chiến lược đồng lắp ráp siêu phân tử xanh và dễ dàng, chúng tôi báo cáo và phát triển một loạt các loại thuốc nhạy sáng đồng lắp ráp trung gian là phân tử nhỏ tự nhiên terpenoid không mang chất mang để tăng cường và phối hợp hóa trị / liệu pháp quang động. Sau khi sàng lọc 17 NSM terpenoid, chúng tôi đã xác định được 11 hợp chất có thể tạo thành các NP NSM-Ce6 đồng lắp ráp với kích thước thuốc có thể điều chỉnh. Phân tích các nano-lắp ráp trung gian đại diện cho axit betulonic (BC) (NP BC-Ce6) cho thấy hiệu quả cao của chiến lược đồng lắp ráp và làm nổi bật tiềm năng to lớn của NSM như các nền tảng phân phối thuốc mới. Thông qua mô phỏng động học phân tử và các tính toán lý thuyết, chúng tôi làm sáng tỏ bí ẩn của quá trình đồng lắp ráp, chỉ ra rằng sự sắp xếp đồng phẳng tuyến tính của các đơn vị dimer BC chủ yếu chịu trách nhiệm cho sự hình thành hình thái giống như thanh hoặc hình cầu. Trong khi đó, chúng tôi chứng minh rằng khoảng cách năng lượng giảm giữa các trạng thái kích thích đơn và ba (ΔEST) tạo điều kiện thuận lợi cho việc tạo ra các loài oxy phản ứng hiệu quả bằng cách thúc đẩy quá trình tạo ·OH thông qua cơ chế phản ứng quang loại I. Các loại thuốc nano lắp ráp thể hiện nhiều đặc điểm điều trị thuận lợi, đảm bảo hiệu quả chống ung thư kết hợp đáng chú ý, tăng cường và an toàn đáng kể là 93,6% với hiệu quả tiêu hủy khối u cao. Công trình này không chỉ mở rộng khả năng của các vật liệu tự nhiên phân hủy sinh học cho các ứng dụng sinh học rộng rãi mà còn cung cấp một góc nhìn mới cho việc xây dựng các nano-lắp ráp trung gian NSM để điều trị chính xác."} +{"idx": 1870760, "en": "Magnetic hyperthermia is a cancer treatment based on the exposure of magnetic nanoparticles to an alternating magnetic field in order to generate local heat. In this work, 3D cell culture models were prepared to observe the effect that a different number of internalized particles had on the mechanisms of cell death triggered upon the magnetic hyperthermia treatment. Macrophages were selected by their high capacity to uptake nanoparticles. Intracellular nanoparticle concentrations up to 7.5 pg Fe/cell were measured both by elemental analysis and magnetic characterization techniques. Cell viability after the magnetic hyperthermia treatment was decreased to <25% for intracellular iron contents above 1 pg per cell. Theoretical calculations of the intracellular thermal effects that occurred during the alternating magnetic field application indicated a very low increase in the global cell temperature. Different apoptotic routes were triggered depending on the number of internalized particles. At low intracellular magnetic nanoparticle amounts (below 1 pg Fe/cell), the intrinsic route was the main mechanism to induce apoptosis, as observed by the high Bax/Bcl-2 mRNA ratio and low caspase-8 activity. In contrast, at higher concentrations of internalized magnetic nanoparticles (1-7.5 pg Fe/cell), the extrinsic route was observed through the increased activity of caspase-8. Nevertheless, both mechanisms may coexist at intermediate iron concentrations. Knowledge on the different mechanisms of cell death triggered after the magnetic hyperthermia treatment is fundamental to understand the biological events activated by this procedure and their role in its effectiveness.", "gemini": "Nhiệt từ trường là phương pháp điều trị ung thư dựa trên việc tiếp xúc các hạt nano từ tính với từ trường xoay để tạo ra nhiệt cục bộ. Trong nghiên cứu này, các mô hình nuôi cấy tế bào 3D đã được chuẩn bị để quan sát tác động của số lượng hạt nội tại hóa khác nhau đến các cơ chế gây chết tế bào được kích hoạt khi điều trị nhiệt từ trường. Các đại thực bào được lựa chọn nhờ khả năng hấp thụ hạt nano cao của chúng. Nồng độ hạt nano trong tế bào lên đến 7,5 pg Fe / tế bào được đo bằng cả kỹ thuật phân tích nguyên tố và đặc tính từ tính. Độ sống của tế bào sau khi điều trị nhiệt từ trường giảm xuống <25% đối với hàm lượng sắt trong tế bào trên 1 pg trên mỗi tế bào. Các tính toán lý thuyết về các hiệu ứng nhiệt trong tế bào xảy ra trong quá trình áp dụng từ trường xoay cho thấy nhiệt độ tế bào toàn cầu tăng rất thấp. Các con đường chết theo chương trình khác nhau được kích hoạt tùy thuộc vào số lượng hạt nội tại hóa. Ở lượng hạt nano từ tính trong tế bào thấp (dưới 1 pg Fe / tế bào), con đường nội tại là cơ chế chính gây ra quá trình chết theo chương trình, như quan sát thấy ở tỷ lệ mRNA Bax / Bcl-2 cao và hoạt động caspase-8 thấp. Ngược lại, ở nồng độ hạt nano từ tính nội tại hóa cao hơn (1-7,5 pg Fe / tế bào), con đường ngoại tại được quan sát thấy thông qua hoạt động tăng lên của caspase-8. Tuy nhiên, cả hai cơ chế đều có thể cùng tồn tại ở nồng độ sắt trung gian. Kiến thức về các cơ chế gây chết tế bào khác nhau được kích hoạt sau khi điều trị nhiệt từ trường là cơ bản để hiểu các sự kiện sinh học được kích hoạt bởi quy trình này và vai trò của chúng trong hiệu quả của nó."} +{"idx": 1870761, "en": "Hafnium oxide (HfO2)-based ferroelectrics offer remarkable promise for memory and logic devices in view of their compatibility with traditional silicon complementary metal oxide semiconductor (CMOS) technology, high switchable polarization, good endurance, and thickness scalability. These factors have led to a steep rise in the level of research on HfO2 over the past number of years. While measurements on capacitors are promising for understanding macroscopic effects, many open questions regarding the emergence of ferroelectricity and electric field cycling behaviors remain. Continued progress requires information regarding the nanoscale ferroelectric behaviors on the bare surface (i.e., without encapsulation), which is notably absent. To overcome this barrier, we have applied complementary modes of piezoresponse force microscopy with the goal of directly and quantitatively sensing nanoscale ferroelectric behaviors in bare HfO2 thin films. Our results on 8 nm Si-doped HfO2 reveal nanoscale domains of local remnant polarization states exhibiting a weak piezoelectric coupling (deff) in the range 0.6-1.5 pm/V. While we observed localized enhancement of deff during progressive stressing of the bare HfO2 thin film, we did not detect stable polarization switching which is a prerequisite of ferroelectric switching. This result could be explained using polarization switching spectroscopy which revealed antiferroelectric-like switching in the form of pinched hysteresis loops as well as increasing remnant response with repeated cycling. As such, our results offer a promising route for material scientists who want to explore the nanoscale origins of antiferroelectricity and ferroelectric wakeup in HfO2.", "gemini": "Các chất sắt điện gốc oxit hafni (HfO2) mang lại triển vọng đáng chú ý cho các thiết bị bộ nhớ và logic do khả năng tương thích của chúng với công nghệ bán dẫn oxit kim loại bổ sung silicon (CMOS) truyền thống, độ phân cực có thể chuyển đổi cao, độ bền tốt và khả năng mở rộng độ dày. Những yếu tố này đã dẫn đến sự gia tăng mạnh mẽ về mức độ nghiên cứu về HfO2 trong những năm gần đây. Mặc dù các phép đo trên tụ điện rất hứa hẹn cho việc hiểu các hiệu ứng vĩ mô, nhưng vẫn còn nhiều câu hỏi chưa có lời giải về sự xuất hiện của các hành vi sắt điện và chu kỳ trường điện. Tiến trình liên tục đòi hỏi thông tin liên quan đến các hành vi sắt điện ở quy mô nano trên bề mặt trần (tức là không có lớp phủ), đáng chú ý là không có. Để vượt qua rào cản này, chúng tôi đã áp dụng các chế độ bổ sung của kính hiển vi lực phản ứng áp điện với mục tiêu cảm biến trực tiếp và định lượng các hành vi sắt điện ở quy mô nano trong màng mỏng HfO2 trần. Kết quả của chúng tôi trên HfO2 pha tạp Si 8 nm cho thấy các miền ở quy mô nano của các trạng thái phân cực còn lại cục bộ thể hiện sự ghép áp điện yếu (deff) trong phạm vi 0,6-1,5 pm / V. Trong khi chúng tôi quan sát thấy sự gia tăng cục bộ của deff trong quá trình ứng suất tiến triển của màng mỏng HfO2 trần, chúng tôi không phát hiện thấy sự chuyển đổi phân cực ổn định, đây là điều kiện tiên quyết của sự chuyển đổi sắt điện. Kết quả này có thể được giải thích bằng cách sử dụng phổ chuyển đổi phân cực, cho thấy sự chuyển đổi giống sắt điện phản trong dạng vòng trễ bị chụm lại cũng như phản ứng còn lại tăng lên với chu kỳ lặp lại. Như vậy, kết quả của chúng tôi cung cấp một lộ trình đầy hứa hẹn cho các nhà khoa học vật liệu muốn khám phá nguồn gốc ở quy mô nano của sự phản sắt điện và sự thức tỉnh sắt điện trong HfO2."} +{"idx": 1870762, "en": "Hydrogels constructed from folded protein domains are of increasing interest as resilient and responsive biomaterials, but their optimization for applications requires time-consuming and costly molecular design. Here, we explore a complementary approach to control their properties by examining the influence of crosslinking rate on the structure and viscoelastic response of a model hydrogel constructed from photochemically crosslinked bovine serum albumin (BSA). Gelation is observed to follow a heterogeneous nucleation pathway in which BSA monomers crosslink into compact nuclei that grow into fractal percolated networks. Both the viscoelastic response probed by shear rheology and the nanostructure probed by small-angle X-ray scattering (SAXS) are shown to depend on the photochemical crosslinking reaction rate, with increased reaction rates corresponding to higher viscoelastic moduli, lower fractal dimension, and higher fractal cluster size. Reaction rate-dependent changes are shown to be consistent with a transition between diffusion- and rate-limited assembly, and the corresponding changes to viscoelastic response are proposed to arise from the presence of nonfractal depletion regions, as confirmed by SAXS. This controllable nanostructure and viscoelasticity constitute a potential route for the precise control of hydrogel properties, without the need for molecular modification.", "gemini": "Các hydrogel được cấu tạo từ các miền protein gấp nếp đang ngày càng được quan tâm như là các vật liệu sinh học có khả năng phục hồi và phản ứng, nhưng việc tối ưu hóa chúng cho các ứng dụng đòi hỏi thiết kế phân tử tốn thời gian và tốn kém. Ở đây, chúng tôi khám phá một phương pháp tiếp cận bổ sung để kiểm soát các tính chất của chúng bằng cách kiểm tra ảnh hưởng của tốc độ liên kết chéo lên cấu trúc và phản ứng nhớt đàn hồi của một mô hình hydrogel được tạo ra từ albumin huyết thanh bò liên kết chéo quang hóa (BSA). Quá trình tạo gel được quan sát là theo một con đường hạt nhân không đồng nhất trong đó các monome BSA liên kết chéo thành các hạt nhân nhỏ gọn phát triển thành các mạng lưới thấm fractal. Cả phản ứng nhớt đàn hồi được thăm dò bằng lưu biến cắt và cấu trúc nano được thăm dò bằng tán xạ tia X góc nhỏ (SAXS) đều được chứng minh là phụ thuộc vào tốc độ phản ứng liên kết chéo quang hóa, với tốc độ phản ứng tăng tương ứng với mô đun nhớt đàn hồi cao hơn, chiều fractal thấp hơn và kích thước cụm fractal cao hơn. Những thay đổi phụ thuộc vào tốc độ phản ứng được chứng minh là phù hợp với quá trình chuyển đổi giữa lắp ráp khuếch tán và giới hạn tốc độ, và những thay đổi tương ứng đối với phản ứng nhớt đàn hồi được đề xuất là phát sinh từ sự hiện diện của các vùng cạn kiệt không fractal, như được xác nhận bởi SAXS. Cấu trúc nano và độ nhớt đàn hồi có thể kiểm soát này tạo thành một lộ trình tiềm năng để kiểm soát chính xác các tính chất của hydrogel, mà không cần phải sửa đổi phân tử."} +{"idx": 1870763, "en": "A low carbon yield is a major limitation for the use of cellulose-based filaments as carbon fiber precursors. The present study aims to investigate the use of an abundant biopolymer chitosan as a natural charring agent particularly on enhancing the carbon yield of the cellulose-derived carbon fiber. The ionic liquid 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) was used for direct dissolution of cellulose and chitosan and to spin cellulose-chitosan composite fibers through a dry-jet wet spinning process (Ioncell). The homogenous distribution and tight packing of cellulose and chitosan revealed by X-ray scattering experiments enable a synergistic interaction between the two polymers during the pyrolysis reaction, resulting in a substantial increase of the carbon yield and preservation of mechanical properties of cellulose fiber compared to other cobiopolymers such as lignin and xylan.", "gemini": "Năng suất carbon thấp là một hạn chế lớn đối với việc sử dụng các sợi dựa trên cellulose làm tiền chất của sợi carbon. Nghiên cứu hiện nay nhằm mục đích điều tra việc sử dụng chitosan sinh học polymer dồi dào làm tác nhân than hóa tự nhiên, đặc biệt là để tăng năng suất carbon của sợi carbon có nguồn gốc từ cellulose. Dung dịch ion 1,5-diazabicyclo[4.3.0]non-5-enium acetate ([DBNH]OAc) được sử dụng để hòa tan trực tiếp cellulose và chitosan và để kéo sợi hỗn hợp cellulose-chitosan thông qua quy trình kéo sợi ướt bằng tia khô (Ioncell). Sự phân bố đồng nhất và đóng gói chặt chẽ của cellulose và chitosan được tiết lộ bởi các thí nghiệm tán xạ tia X cho phép tương tác hiệp đồng giữa hai polyme trong quá trình phản ứng nhiệt phân, dẫn đến sự gia tăng đáng kể năng suất carbon và bảo toàn các tính chất cơ học của sợi cellulose so với các copolyme khác như lignin và xylan."} +{"idx": 1870764, "en": "Symmetry is highly relevant with various quantities and phenomena in physics. While the translational symmetry breaks at the edges of two-dimensional hexagonal crystalline flakes, it is usually associated with the breaking of central inversion symmetry that is yet to be observed in terms of physical properties. Here, we report an experiment-theory joint study on in-plane compressed single-crystal monolayer WS2 flakes. Although the flakes show a hexagonal appearance with a C6 symmetry, our density functional theory calculations predict that their in-plane strain, geometric structure, work-function, energy bandgap, and mechanical modulus are nonequivalent among the triangular regions with different edge terminations at the atomic scale, and the flakes exhibit self-patterns with a C3 symmetry. Such nonequivalence of physical properties and concomitant self-patterns persist even in a 50 μm-sized monolayer WS2, observed using atomic force microscopy. This indicates that the symmetry arising from the atomic geometry could preserve up to tens of microns for both geometric and properties of the flake, regardless of its mesoscopic geometry, i.e., C6 here. Such a detectable mesoscopic scale and symmetric nano- to mesoscale patterns provide promising building blocks for 2D materials and devices and also allow edge terminations of 2D flakes to be directly distinguished.", "gemini": "Tính đối xứng có liên quan cao với các đại lượng và hiện tượng khác nhau trong vật lý. Mặc dù tính đối xứng chuyển động bị phá vỡ ở các cạnh của các mảnh tinh thể lục giác hai chiều, nhưng nó thường liên quan đến sự phá vỡ của tính đối xứng đảo ngược trung tâm mà vẫn chưa được quan sát thấy về mặt tính chất vật lý. Ở đây, chúng tôi báo cáo một nghiên cứu chung giữa lý thuyết và thực nghiệm về các mảnh WS2 đơn tinh thể nén trong mặt phẳng. Mặc dù các mảnh có hình lục giác với đối xứng C6, nhưng các tính toán lý thuyết hàm mật độ của chúng tôi dự đoán rằng ứng suất trong mặt phẳng, cấu trúc hình học, hàm công, khoảng cách năng lượng và mô đun cơ học của chúng không tương đương nhau giữa các vùng tam giác có đầu mút cạnh khác nhau ở cấp độ nguyên tử và các mảnh thể hiện các mẫu tự với đối xứng C3. Sự không tương đương như vậy của các tính chất vật lý và các mẫu tự đi kèm vẫn tồn tại ngay cả trong một lớp đơn WS2 có kích thước 50 μm, được quan sát bằng kính hiển vi lực nguyên tử. Điều này chỉ ra rằng tính đối xứng phát sinh từ hình học nguyên tử có thể bảo toàn tới hàng chục micron đối với cả hình học và tính chất của mảnh, bất kể hình dạng trung quan của nó, tức là C6 ở đây. Một thang đo trung quan có thể phát hiện được như vậy và các mẫu nano đến trung quan đối xứng cung cấp các khối xây dựng đầy hứa hẹn cho các vật liệu và thiết bị 2D và cũng cho phép phân biệt trực tiếp các đầu mút cạnh của các mảnh 2D."} +{"idx": 1870765, "en": "Transformations of biomass burning brown carbon aerosols (BB-BrC) over their diurnal lifecycle are currently not well studied. In this study, the aging of BB tar proxy aerosols processed by NO3• under dark conditions followed by the photochemical OH• reaction and photolysis were investigated in tandem flow reactors. The results show that O3 oxidation in the dark diminishes light absorption of wood tar aerosols, resulting in higher particle single-scattering albedo (SSA). NO3• reactions augment the mass absorption coefficient (MAC) of the aerosols by a factor of 2-3 by forming secondary chromophores, such as nitroaromatic compounds (NACs) and organonitrates. Subsequent OH• oxidation and direct photolysis both decompose the organic nitrates (ONs, representing bulk functionalities of NACs and organonitrates) in the NO3•-aged wood tar aerosols, thus decreasing particle absorption. Moreover, NACs degrade faster than organonitrates by photochemical aging. The NO3•-aged wood tar aerosols are more susceptible to photolysis than to OH• reactions. The photolysis lifetimes for the ONs and for the absorbance of the NO3•-aged aerosols are on the order of hours under typical solar irradiation, while the absorption and ON lifetimes toward OH• oxidation are substantially longer. Overall, nighttime aging via NO3• reactions increases the light absorption of wood tar aerosols and shortens their absorption lifetime under daytime conditions.", "gemini": "Hiện nay, sự chuyển hóa của các hạt carbon nâu do đốt sinh khối (BB-BrC) trong suốt vòng đời ban ngày của chúng vẫn chưa được nghiên cứu đầy đủ. Trong nghiên cứu này, quá trình lão hóa của các hạt aerosol proxy nhựa gỗ BB được xử lý bằng NO3• trong điều kiện tối, tiếp theo là phản ứng OH• quang hóa và quang phân đã được nghiên cứu trong các lò phản ứng dòng song song. Kết quả cho thấy quá trình oxy hóa O3 trong bóng tối làm giảm sự hấp thụ ánh sáng của các hạt aerosol nhựa gỗ, dẫn đến độ phản xạ tán xạ đơn của hạt cao hơn (SSA). Các phản ứng NO3• làm tăng hệ số hấp thụ khối lượng (MAC) của các hạt aerosol lên gấp 2-3 lần bằng cách tạo thành các chất tạo màu thứ cấp, chẳng hạn như các hợp chất nitro thơm (NAC) và các hợp chất nitrat hữu cơ. Quá trình oxy hóa OH• tiếp theo và quang phân trực tiếp đều phân hủy các nitrat hữu cơ (ON, đại diện cho các chức năng chính của NAC và nitrat hữu cơ) trong các hạt aerosol nhựa gỗ đã lão hóa NO3•, do đó làm giảm sự hấp thụ hạt. Hơn nữa, NAC phân hủy nhanh hơn nitrat hữu cơ do quá trình lão hóa quang hóa. Các hạt aerosol nhựa gỗ đã lão hóa NO3• dễ bị quang phân hơn là các phản ứng OH•. Tuổi thọ quang phân đối với ON và đối với độ hấp thụ của các hạt aerosol đã lão hóa NO3• vào khoảng hàng giờ dưới bức xạ mặt trời thông thường, trong khi thời gian hấp thụ và ON đối với quá trình oxy hóa OH• dài hơn đáng kể. Nhìn chung, quá trình lão hóa ban đêm thông qua các phản ứng NO3• làm tăng sự hấp thụ ánh sáng của các hạt aerosol nhựa gỗ và rút ngắn thời gian hấp thụ của chúng trong điều kiện ban ngày."} +{"idx": 1870766, "en": "The occurrence of per- and polyfluoroalkyl substances (PFASs) in aquatic ecosystems is a global concern because of their persistence, potential bioaccumulation, and toxicity. In this study, we investigated a PFAS-contaminated pond in Sweden to assess the cross-boundary transfer of PFASs from the aquatic environment to the riparian zone via emergent aquatic insects. Aquatic and terrestrial invertebrates, surface water, sediments, soils, and plants were analyzed for 24 PFASs including branched isomers. Stable isotope analysis of carbon and nitrogen was performed to elucidate the importance of diet and trophic position for PFAS uptake. We present the first evidence that PFASs can propagate to the riparian food web via aquatic emergent insects. Elevated Σ24PFAS concentrations were found in aquatic insect larvae, such as dragon- and damselflies, ranging from 1100 to 4600 ng g-1 dry weight (dw), and remained high in emerged adults (120-3500 ng g-1 dw), indicating exposure risks for top predators that prey in riparian zones. In terrestrial invertebrate consumers, PFAS concentrations increased with the degree of aquatic-based diet and at higher trophic levels. Furthermore, stable isotope data together with calculated bioaccumulation factors indicated that bioconcentration of PFASs was the major pathway of exposure in the aquatic food web and bioaccumulation in the riparian food web.", "gemini": "Sự xuất hiện của các chất perfluoroalkyl và polyfluoroalkyl (PFAS) trong các hệ sinh thái dưới nước là mối quan tâm toàn cầu vì tính bền vững, khả năng tích lũy sinh học và độc tính của chúng. Trong nghiên cứu này, chúng tôi đã điều tra một ao bị ô nhiễm PFAS ở Thụy Điển để đánh giá sự di chuyển xuyên biên giới của PFAS từ môi trường dưới nước đến vùng ven bờ thông qua côn trùng thủy sinh mới nổi. Các loài động vật không xương sống dưới nước và trên cạn, nước mặt, trầm tích, đất và thực vật đã được phân tích đối với 24 PFAS bao gồm các đồng phân nhánh. Phân tích đồng vị ổn định của carbon và nitơ đã được thực hiện để làm sáng tỏ tầm quan trọng của chế độ ăn và vị trí dinh dưỡng đối với quá trình hấp thụ PFAS. Chúng tôi trình bày bằng chứng đầu tiên cho thấy PFAS có thể lan truyền đến lưới thức ăn ven bờ thông qua côn trùng thủy sinh mới nổi. Nồng độ Σ24PFAS tăng cao đã được tìm thấy trong ấu trùng côn trùng thủy sinh, chẳng hạn như chuồn chuồn và chuồn chuồn kim, trong khoảng từ 1100 đến 4600 ng g-1 trọng lượng khô (dw), và vẫn ở mức cao ở những con trưởng thành mới xuất hiện (120-3500 ng g-1 dw), cho thấy rủi ro phơi nhiễm đối với những loài ăn thịt đầu bảng săn mồi ở vùng ven bờ. Ở những loài động vật không xương sống trên cạn, nồng độ PFAS tăng theo mức độ ăn dựa trên nguồn nước và ở các mức dinh dưỡng cao hơn. Hơn nữa, dữ liệu đồng vị ổn định cùng với các yếu tố tích lũy sinh học được tính toán chỉ ra rằng quá trình tích lũy sinh học của PFAS là con đường chính để tiếp xúc trong lưới thức ăn dưới nước và quá trình tích lũy sinh học trong lưới thức ăn ven bờ."} +{"idx": 1870767, "en": "A new molecular donor-acceptor-donor (D-A-D) triad, comprised of an electron deficient 1,4,5,8-naphthalene tetracarboxylic diimide (NDI) unit covalently connected to two flanking photosensitizers, i.e., a bis-heteroleptic Ru(II) complex of 1,10-phenanthroline and pyridine triazole hybrid ligand, is described. The single crystal X-ray structure of the perchlorate salt of the triad demonstrates that the electron deficient NDI unit can act as a host for anions via anion-π interaction. Detailed solution-state studies indicate that fluoride selectively interacts with the D-A-D triad to form a dianionic NDI, NDI2-, via a radical anion, NDI•-. On the contrary, cyanide reduces the NDI moiety to NDI•-, as confirmed by UV-vis, NMR, and EPR spectroscopy. Further, femtosecond transient absorption spectroscopic studies reveal a low luminescence quantum yield of the D-A-D triad attributable to the photoinduced electron transfer (PET) process from the photoactive Ru(II) center to the NDI unit. Interestingly, the triad displays \"OFF-ON\" luminescence behavior in the presence of fluoride by restoring the Ru(II) to phenanthroline/pyridine-triazole-based MLCT emission, whereas cyanide fails to show a similar property due to a different redox process operational in the latter. The reduction of NDI in the presence of fluoride and cyanide in different polar solvents indicates that involvement of such deprotonated solvents in the electron transfer mechanism may not be operative in our present system. Low-temperature kinetic studies support the formation of a charge transfer associative transient species, which likely allows overcoming the thermodynamically uphill barrier for the direct electron transfer mechanism.", "gemini": "Một bộ ba phân tử cho-nhận-cho (D-A-D) mới, bao gồm một đơn vị imid tetracacboxylic diimide (NDI) thiếu điện tử 1,4,5,8 được kết nối cộng hóa trị với hai chất nhạy sáng bao quanh, tức là một phức chất Ru(II) dị dị hợp của 1,10-phenanthroline và phối tử lai pyridine triazole, được mô tả. Cấu trúc tinh thể đơn của muối perchlorat của bộ ba cho thấy đơn vị NDI thiếu điện tử có thể hoạt động như một vật chủ cho các anion thông qua tương tác anion-π. Các nghiên cứu chi tiết về trạng thái dung dịch chỉ ra rằng fluoride tương tác chọn lọc với bộ ba D-A-D để tạo thành NDI dianion, NDI2-, thông qua một anion gốc tự do, NDI•-. Ngược lại, xyanua khử phần NDI thành NDI•-, như được xác nhận bằng phổ UV-vis, NMR và EPR. Hơn nữa, các nghiên cứu quang phổ hấp thụ tạm thời femto giây cho thấy hiệu suất lượng tử phát quang thấp của bộ ba D-A-D có thể quy cho quá trình chuyển electron cảm ứng quang (PET) từ trung tâm Ru(II) hoạt động quang sang đơn vị NDI. Điều thú vị là bộ ba này thể hiện hành vi phát quang \"TẮT-BẬT\" khi có mặt fluoride bằng cách khôi phục Ru(II) thành phát xạ MLCT dựa trên phenanthroline/pyridine-triazole, trong khi xyanua không thể hiện tính chất tương tự do một quá trình oxy hóa khử khác hoạt động trong quá trình sau. Sự khử NDI khi có mặt fluoride và xyanua trong các dung môi phân cực khác nhau chỉ ra rằng sự tham gia của các dung môi bị khử proton như vậy trong cơ chế chuyển electron có thể không hoạt động trong hệ thống hiện tại của chúng tôi. Các nghiên cứu động học ở nhiệt độ thấp hỗ trợ sự hình thành của các loài tạm thời liên kết chuyển điện tích, có khả năng cho phép vượt qua rào cản về nhiệt động lực học cho cơ chế chuyển electron trực tiếp."} +{"idx": 1870768, "en": "Hundreds of nuclear, cytoplasmic, and mitochondrial proteins within multicellular eukaryotes have hydroxyl groups of specific serine and threonine residues modified by the monosaccharide N-acetylglucosamine (GlcNAc). This modification, known as O-GlcNAc, has emerged as a central regulator of both cell physiology and human health. A key emerging function of O-GlcNAc appears to be to regulate cellular protein homeostasis. We previously showed, using overexpressed model proteins, that O-GlcNAc modification can occur cotranslationally and that this process prevents premature degradation of such nascent polypeptide chains. Here, we use tandem metabolic engineering strategies to label endogenously occurring nascent polypeptide chains within cells using O-propargyl-puromycin (OPP) and target the specific subset of nascent chains that are cotranslationally glycosylated with O-GlcNAc by metabolic saccharide engineering using tetra-O-acetyl-2-N-azidoacetyl-2-deoxy-d-galactopyranose (Ac4GalNAz). Using various combinations of sequential chemoselective ligation strategies, we go on to tag these analytes with a series of labels, allowing us to define conditions that enable their robust labeling. Two-step enrichment of these glycosylated nascent chains, combined with shotgun proteomics, allows us to identify a set of endogenous cotranslationally O-GlcNAc modified proteins. Using alternative targeted methods, we examine three of these identified proteins and further validate their cotranslational O-GlcNAcylation. These findings detail strategies to enable isolation and identification of extremely low abundance endogenous analytes present within complex protein mixtures. Moreover, this work opens the way to studies directed at understanding the roles of O-GlcNAc and other cotranslational protein modifications and should stimulate an improved understanding of the role of O-GlcNAc in cytoplasmic protein quality control and proteostasis.", "gemini": "Hàng trăm protein nhân, tế bào chất và ty thể trong các sinh vật nhân chuẩn đa bào có các nhóm hydroxyl của các gốc serine và threonine cụ thể được sửa đổi bởi monosaccharide N-acetylglucosamine (GlcNAc). Sửa đổi này, được gọi là O-GlcNAc, đã nổi lên như một chất điều hòa trung tâm của cả sinh lý tế bào và sức khỏe con người. Một chức năng mới nổi của O-GlcNAc dường như là điều chỉnh cân bằng protein tế bào. Trước đây, chúng tôi đã chỉ ra, bằng cách sử dụng các protein mô hình biểu hiện quá mức, rằng sửa đổi O-GlcNAc có thể xảy ra cùng với quá trình dịch mã và quá trình này ngăn ngừa sự phân hủy sớm của các chuỗi polypeptide mới hình thành. Ở đây, chúng tôi sử dụng các chiến lược kỹ thuật chuyển hóa song song để dán nhãn các chuỗi polypeptide mới hình thành có nguồn gốc nội sinh trong tế bào bằng cách sử dụng O-propargyl-puromycin (OPP) và nhắm vào tập hợp con cụ thể của các chuỗi mới hình thành được glycosyl hóa cùng với quá trình dịch mã với O-GlcNAc bằng kỹ thuật chuyển hóa saccharide sử dụng tetra-O-acetyl-2-N-azidoacetyl-2-deoxy-d-galactopyranose (Ac4GalNAz). Sử dụng nhiều kết hợp khác nhau của các chiến lược liên kết hóa học chọn lọc tuần tự, chúng tôi tiếp tục gắn các chất phân tích này bằng một loạt các nhãn, cho phép chúng tôi xác định các điều kiện giúp chúng có thể gắn nhãn mạnh mẽ. Sự làm giàu hai bước của các chuỗi mới hình thành được glycosyl hóa này, kết hợp với phép đo độ phân giải cao về proteomics, cho phép chúng tôi xác định một bộ các protein được sửa đổi bằng O-GlcNAc cùng với quá trình dịch mã nội sinh. Sử dụng các phương pháp nhắm mục tiêu thay thế, chúng tôi kiểm tra ba trong số các protein đã xác định này và xác nhận thêm quá trình O-GlcNAcylation cùng với quá trình dịch mã của chúng. Những phát hiện này trình bày chi tiết các chiến lược để cho phép phân lập và xác định các chất phân tích nội sinh có hàm lượng cực thấp có trong các hỗn hợp protein phức tạp. Hơn nữa, công trình này mở đường cho các nghiên cứu hướng đến mục đích hiểu được vai trò của O-GlcNAc và các sửa đổi protein cùng với quá trình dịch mã khác và sẽ thúc đẩy sự hiểu biết tốt hơn về vai trò của O-GlcNAc trong kiểm soát chất lượng protein tế bào chất và sự ổn định của protein."} +{"idx": 1870769, "en": "Petroleum resource development is a significant contributor of greenhouse gas emissions to the atmosphere. A potential source of emissions may result from stray gas migration. However, its contribution to overall emissions and potential groundwater contamination is unknown, and quantification of flow and dissolution of stray gas is required. The environmental expression of stray gas was investigated using an intermediate-scale (150 × 150 × 2 cm3), two-dimensional flow cell packed in both homogeneous and heterogeneous sand configurations allowing for visualization and measurement of gas movement, collection of aqueous samples, and real-time measurement of gas fluxes escaping the surface of the sand. Results show that gas is transported to the surface of the system via varying dominant discontinuous conduits for flow dictated by geology, leading to surface expression that can be greater or less than the leakage rate of gas. This suggests that surface expression is not directly indicative of the expanse and magnitude of stray gas migration leaks. It was found that 35-39% of the methane was released to the aqueous phase and 61-65% to the atmosphere. The results underscore that subsurface characteristics and gas flow are the key drivers for the overall expression of stray gas in unconsolidated sand aquifers.", "gemini": "Phát triển nguồn tài nguyên dầu mỏ là một yếu tố đóng góp đáng kể vào lượng khí nhà kính thải ra bầu khí quyển. Một nguồn phát thải tiềm ẩn có thể là do sự di chuyển của khí đi lạc. Tuy nhiên, sự đóng góp của nó vào tổng lượng khí thải và khả năng ô nhiễm nước ngầm vẫn chưa được biết và cần phải định lượng lưu lượng và độ hòa tan của khí đi lạc. Biểu hiện môi trường của khí đi lạc đã được nghiên cứu bằng cách sử dụng một tế bào dòng hai chiều có quy mô trung gian (150 × 150 × 2 cm3), được đóng gói trong cả cấu hình cát đồng nhất và không đồng nhất cho phép hình dung và đo chuyển động của khí, thu thập mẫu nước và đo thời gian thực các luồng khí thoát ra khỏi bề mặt của cát. Kết quả cho thấy khí được vận chuyển đến bề mặt của hệ thống thông qua các ống dẫn không liên tục chiếm ưu thế khác nhau cho dòng chảy được quyết định bởi địa chất, dẫn đến biểu hiện bề mặt có thể lớn hơn hoặc nhỏ hơn tốc độ rò rỉ khí. Điều này cho thấy rằng biểu hiện bề mặt không chỉ ra trực tiếp về phạm vi và quy mô rò rỉ khí đi lạc. Người ta thấy rằng 35-39% metan được giải phóng vào pha nước và 61-65% vào khí quyển. Kết quả nhấn mạnh rằng các đặc điểm dưới bề mặt và dòng khí là động lực chính cho biểu hiện tổng thể của khí đi lạc trong các tầng chứa nước cát không cố kết."} +{"idx": 1870770, "en": "Low-dimensional perovskite-related metal halides have emerged as a new class of light-emitting materials with tunable broadband emission from self-trapped excitons (STEs). Although various types of low-dimensional structures have been developed, fundamental understating of the structure-property relationships for this class of materials is still very limited, and further improvement of their optical properties remains greatly important. Here, we report a significant pressure-induced photoluminescence (PL) enhancement in a one-dimensional hybrid metal halide C4N2H14PbBr4, and the underlying mechanisms are investigated using in situ experimental characterization and first-principles calculations. Under a gigapascal pressure scale, the PL quantum yields (PLQYs) were quantitatively determined to show a dramatic increase from the initial value of 20% at ambient conditions to over 90% at 2.8 GPa. With in situ characterization of photophysical properties and theoretical analysis, we found that the PLQY enhancement was mainly attributed to the greatly suppressed nonradiative decay. Pressure can effectively tune the energy level of self-trapped states and increase the exciton binding energy, which leads to a larger Stokes shift. The resulting highly localized excitons with stronger binding reduce the probability for carrier scattering, to result in the significantly suppressed nonradiative decay. Our findings clearly show that the characteristics of STEs in low-dimensional metal halides can be well-tuned by external pressure, and enhanced optical properties can be achieved.", "gemini": "Các halide kim loại liên quan đến perovskite kích thước thấp đã nổi lên như một lớp vật liệu phát sáng mới với khả năng phát xạ băng thông rộng có thể điều chỉnh từ các exciton tự bẫy (STE). Mặc dù nhiều loại cấu trúc kích thước thấp đã được phát triển, nhưng sự hiểu biết cơ bản về mối quan hệ cấu trúc-tính chất của lớp vật liệu này vẫn còn rất hạn chế và việc cải thiện thêm các tính chất quang học của chúng vẫn rất quan trọng. Tại đây, chúng tôi báo cáo về sự tăng cường quang phát quang (PL) do áp suất đáng kể trong một halide kim loại lai một chiều C4N2H14PbBr4 và các cơ chế cơ bản được nghiên cứu bằng cách sử dụng đặc tính thử nghiệm tại chỗ và các phép tính nguyên lý đầu tiên. Trong thang áp suất gigapascal, năng suất lượng tử PL (PLQY) đã được xác định định lượng để cho thấy sự gia tăng đáng kể từ giá trị ban đầu là 20% ở điều kiện môi trường lên hơn 90% ở 2,8 GPa. Với đặc tính quang vật lý tại chỗ và phân tích lý thuyết, chúng tôi thấy rằng sự tăng cường PLQY chủ yếu là do sự phân rã không bức xạ bị ức chế rất nhiều. Áp suất có thể điều chỉnh hiệu quả mức năng lượng của các trạng thái tự bẫy và tăng năng lượng liên kết exciton, dẫn đến độ dịch Stokes lớn hơn. Các exciton cục bộ hóa cao kết quả với liên kết mạnh hơn làm giảm khả năng phân tán của hạt tải, dẫn đến sự phân rã không bức xạ bị ức chế đáng kể. Những phát hiện của chúng tôi cho thấy rõ ràng rằng các đặc tính của STE trong các halide kim loại kích thước thấp có thể được điều chỉnh tốt bằng áp suất bên ngoài và có thể đạt được các tính chất quang học được cải thiện."} +{"idx": 1870771, "en": "Localizing light to nanoscale volumes through nanoscale resonators that are low loss and precisely tailored in spectrum to properties of matter is crucial for classical and quantum light sources, cavity QED, molecular spectroscopy, and many other applications. To date, two opposite strategies have been identified: to use either plasmonics with deep subwavelength confinement yet high loss and very poor spectral control or instead microcavities with exquisite quality factors yet poor confinement. In this work we realize hybrid plasmonic-photonic resonators that enhance the emission of single quantum dots, profiting from both plasmonic confinement and microcavity quality factors. Our experiments directly demonstrate how cavity and antenna jointly realize large cooperative Purcell enhancements through interferences. These can be controlled to engineer arbitrary Fano lineshapes in the local density of optical states.", "gemini": "Việc định vị ánh sáng đến thể tích nano thông qua các cộng hưởng nano có tổn thất thấp và điều chỉnh chính xác trong phổ với các tính chất của vật chất là rất quan trọng đối với các nguồn sáng cổ điển và lượng tử, QED khoang, quang phổ phân tử và nhiều ứng dụng khác. Cho đến nay, hai chiến lược đối lập đã được xác định: sử dụng plasmonic với sự giới hạn dưới bước sóng sâu nhưng tổn thất cao và kiểm soát quang phổ rất kém hoặc thay vào đó là các vi khoang có các hệ số chất lượng tuyệt vời nhưng sự giới hạn kém. Trong công trình này, chúng tôi nhận ra các cộng hưởng plasmonic-photonic lai tăng cường sự phát xạ của các chấm lượng tử đơn, hưởng lợi từ cả sự giới hạn plasmonic và các hệ số chất lượng vi khoang. Các thí nghiệm của chúng tôi chứng minh trực tiếp cách khoang và ăng-ten cùng nhau nhận ra các sự tăng cường Purcell hợp tác lớn thông qua nhiễu. Những điều này có thể được kiểm soát để thiết kế các hình dạng đường Fano tùy ý trong mật độ trạng thái quang học cục bộ."} +{"idx": 1870772, "en": "Luminescent thermochromic materials with a dramatic shift of emission band under different temperatures are highly desirable in temperature sensing fields. However, the design of the synthesis of such compounds remains a great challenge. In this work, two new luminescent thermochromic silver iodides, (emIm)Ag3I4 (1) and (emIm)Ag2I3 (2) (emIm = 1-ethyl-3-methyl imidazole), have been synthesized under solvothermal conditions. Compound 1 features a [Ag3I4]- anionic layer, while compound 2 possesses an infinite [Ag2I3]- chain structure, both of which are charge balanced by emIm+ cations. Particularly, they display luminescent thermochromism with a significant wavelength shift of emission maximum with temperature change. They represent rare examples of infinite layered or chain silver iodides that show luminescent thermochromism. Furthermore, the results indicate that compounds 1 and 2 are promising wavelength-dependent luminescent thermometers.", "gemini": "Vật liệu nhiệt phát quang với sự dịch chuyển mạnh của dải phát xạ dưới các nhiệt độ khác nhau được mong muốn rất cao trong lĩnh vực cảm biến nhiệt độ. Tuy nhiên, việc thiết kế tổng hợp các hợp chất như vậy vẫn là một thách thức lớn. Trong công trình này, hai loại bạc iodua nhiệt phát quang mới, (emIm) Ag3I4 (1) và (emIm) Ag2I3 (2) (emIm = 1-ethyl-3-methyl imidazole), đã được tổng hợp trong điều kiện dung môi nhiệt. Hợp chất 1 có một lớp anion [Ag3I4]-, trong khi hợp chất 2 sở hữu cấu trúc chuỗi vô hạn [Ag2I3]-, cả hai đều được cân bằng điện tích bởi các cation emIm+. Đặc biệt, chúng thể hiện nhiệt phát quang với sự dịch chuyển bước sóng đáng kể của phát xạ cực đại khi nhiệt độ thay đổi. Chúng đại diện cho những ví dụ hiếm hoi về các bạc iodua dạng lớp hoặc chuỗi vô hạn thể hiện nhiệt phát quang. Hơn nữa, kết quả chỉ ra rằng các hợp chất 1 và 2 là nhiệt kế phát quang phụ thuộc vào bước sóng đầy hứa hẹn."} +{"idx": 1870773, "en": "The lithium cation Li+-catalyzed Diels-Alder (DA) reactions of benzene toward a series of acetylenes of improved nucleophilicity can be described within the context of the molecular electron density theory (MEDT) at the ωB97XD/6-311G(d,p) level. Conceptual density functional theory indices characterize the crown ether solvated complex benzene-lithium Bz-Li-Cro as a superelectrophile. Coordination of a lithium cation to benzene does not change substantially the electron localization function electronic structure of benzene. The DA reaction of Bz-Li-Cro with acetylene shows a reduction of the energy of activation of 6.9 kcal·mol-1, which is not sufficient for the reaction to take place, thus demanding the participation of strong nucleophilic acetylenes. DA reactions of complexes Bz-M-Cro (M = Li, Na, and K) are decelerated with the decrease of the ionization potential of the alkali metal. The one-step mechanism of these lithium cation Li+-catalyzed DA reactions changes to a two-step one for the reaction with dimethyl propynamine. The present MEDT study proves that analysis of the electrophilicity and nucleophilicity indices is an excellent tool for experimental organic chemists to understand, even to predict, the chemical organic reactivity.", "gemini": "Các phản ứng Diels-Alder (DA) xúc tác bởi cation lithium Li+ của benzen hướng đến một loạt các acetylen có tính nucleophil được cải thiện có thể được mô tả trong bối cảnh của lý thuyết mật độ electron phân tử (MEDT) ở mức ωB97XD/6-311G(d,p). Các chỉ số lý thuyết hàm mật độ khái niệm đặc trưng cho phức chất benzen-liti Bz-Li-Cro hòa tan trong ete vòng là một siêu điện tích. Sự phối hợp của một cation lithium với benzen không làm thay đổi đáng kể cấu trúc điện tử của hàm cục bộ hóa electron của benzen. Phản ứng DA của Bz-Li-Cro với acetylen cho thấy sự giảm năng lượng hoạt hóa là 6,9 kcal·mol-1, điều này không đủ để phản ứng diễn ra, do đó đòi hỏi sự tham gia của các acetylen có tính nucleophil mạnh. Các phản ứng DA của các phức chất Bz-M-Cro (M = Li, Na và K) chậm lại khi thế ion hóa của kim loại kiềm giảm. Cơ chế một bước của các phản ứng DA xúc tác bởi cation lithium Li+ này thay đổi thành cơ chế hai bước đối với phản ứng với dimethyl propynamin. Nghiên cứu MEDT hiện tại chứng minh rằng việc phân tích các chỉ số ái điện tử và ái nucleophil là một công cụ tuyệt vời để các nhà hóa học hữu cơ thực nghiệm hiểu, thậm chí dự đoán, phản ứng hữu cơ hóa học."} +{"idx": 1870774, "en": "Little is known about the underlying mechanisms governing the bioaccumulation of uranium (U) in aquatic insects. We experimentally parameterized conditional rate constants for aqueous U uptake, dietary U uptake, and U elimination for the aquatic baetid mayfly Neocloeon triangulifer. Results showed that this species accumulates U from both the surrounding water and diet, with waterborne uptake prevailing. Elevated dietary U concentrations decreased feeding rates, presumably by altering food palatability or impairing the mayfly's digestive processes, or both. Nearly 90% of the accumulated U was eliminated within 24 h after the waterborne exposure ceased, reflecting the desorption of weakly bound U from the insect's integument. To examine whether the experimentally derived rate constants for N. triangulifer could be generalized to baetid mayflies, mayfly U concentrations were predicted using the water chemistry and U measured in periphyton from springs in Grand Canyon (United States) and were compared to U concentrations in spring-dwelling mayflies. Predicted and observed mayfly U concentrations were in good agreement. Under the modeled site-specific conditions, waterborne U uptake accounted for 52-93% of the bioaccumulated U. U accumulation was limited in these wild populations due to a combination of factors including low concentrations of bioavailable dissolved U species, slow U uptake rates from food, and fast U elimination.", "gemini": "Người ta biết rất ít về các cơ chế cơ bản chi phối sự tích tụ sinh học của urani (U) trong côn trùng thủy sinh. Chúng tôi đã tham số hóa theo phương pháp thực nghiệm các hằng số tỷ lệ có điều kiện đối với quá trình hấp thụ U trong nước, hấp thụ U trong chế độ ăn và đào thải U đối với loài phù du cánh cứng thủy sinh Neocloeon triangulifer. Kết quả cho thấy loài này tích tụ U từ cả nước xung quanh và chế độ ăn, trong đó quá trình hấp thụ qua đường nước là chủ yếu. Nồng độ U trong chế độ ăn cao làm giảm tỷ lệ ăn, có lẽ là do làm thay đổi độ ngon miệng của thức ăn hoặc làm suy yếu quá trình tiêu hóa của phù du cánh cứng hoặc cả hai. Gần 90% U tích tụ đã được đào thải trong vòng 24 giờ sau khi quá trình phơi nhiễm qua đường nước chấm dứt, phản ánh sự giải hấp phụ của U liên kết yếu từ lớp vỏ của côn trùng. Để kiểm tra xem các hằng số tỷ lệ thu được theo phương pháp thực nghiệm đối với N. triangulifer có thể được khái quát hóa cho phù du cánh cứng hay không, nồng độ U của phù du cánh cứng đã được dự đoán bằng cách sử dụng hóa học nước và U đo được trong lớp màng sinh học từ các suối ở Grand Canyon (Hoa Kỳ) và được so sánh với nồng độ U ở phù du cánh cứng sống trong suối. Nồng độ U của phù du cánh cứng được dự đoán và quan sát thấy phù hợp với nhau. Theo các điều kiện cụ thể của địa điểm được mô hình hóa, quá trình hấp thụ U qua đường nước chiếm 52-93% U tích tụ sinh học. Sự tích tụ U bị hạn chế ở những quần thể hoang dã này do sự kết hợp của các yếu tố bao gồm nồng độ thấp các loài U hòa tan có thể hấp thụ sinh học, tốc độ hấp thụ U chậm từ thức ăn và quá trình đào thải U nhanh."} +{"idx": 1870775, "en": "Parent and modified mycotoxin analysis remains a challenge because of their chemical diversity, the presence of isomeric forms, and the lack of analytical standards. The creation and application of a collision cross section (CCS) database for mycotoxins may bring new opportunities to overcome these analytical challenges. However, it is still an open question whether common CCS databases can be used independently from the instrument type and ion mobility mass spectrometry (IM-MS) technologies, which utilize different methodologies for determining the gas-phase mobility. Here, we demonstrated the reproducibility of CCS measurements for mycotoxins in an interlaboratory study (average RSD 0.14% ± 0.079) and across different traveling wave IM-MS (TWIMS) systems commercially available (ΔCCS% < 2). The separation in the drift time dimension of critical pairs of isomers for modified mycotoxins was also achieved. In addition, the comparison of measured and predicted CCS values, including regulated and emerging mycotoxins, was addressed.", "gemini": "Phân tích độc tố nấm mốc gốc và đã biến đổi vẫn là một thách thức vì sự đa dạng về mặt hóa học, sự hiện diện của các dạng đồng phân và thiếu các tiêu chuẩn phân tích. Việc tạo và ứng dụng cơ sở dữ liệu về tiết diện va chạm (CCS) cho độc tố nấm mốc có thể mang lại những cơ hội mới để vượt qua những thách thức phân tích này. Tuy nhiên, vẫn còn một câu hỏi chưa có lời giải là liệu có thể sử dụng các cơ sở dữ liệu CCS phổ biến độc lập với loại thiết bị và công nghệ phổ khối di động ion (IM-MS), vốn sử dụng các phương pháp khác nhau để xác định độ di động trong pha khí hay không. Ở đây, chúng tôi đã chứng minh khả năng tái tạo của các phép đo CCS đối với độc tố nấm mốc trong một nghiên cứu liên phòng thí nghiệm (RSD trung bình 0,14% ± 0,079) và trên các hệ thống IM-MS sóng chạy khác nhau có sẵn trên thị trường (ΔCCS% < 2). Sự tách biệt trong chiều thời gian trôi dạt của các cặp đồng phân quan trọng đối với độc tố nấm mốc đã biến đổi cũng đạt được. Ngoài ra, vấn đề so sánh các giá trị CCS đo được và dự đoán, bao gồm các độc tố nấm mốc được quy định và mới nổi, đã được giải quyết."} +{"idx": 1870776, "en": "Heat stress is a major abiotic stress that significantly affects plant growth and productivity. Plants have, however, evolved complex adaptive mechanisms to cope with heat stress. MicroRNAs (miRNAs) are important molecules that regulate gene expression through the post-transcriptional degradation of target mRNA molecules or by repressing translation. Plant miRNAs play essential roles in development and a variety of stress responses. Recent advances in high-throughput sequencing technologies have enabled the identification and characterization of an increasing number of heat-responsive miRNAs in diverse plant species. Heat-regulated miRNAs combined with their target genes constitute large regulatory networks that control various metabolic pathways, including protein refolding, antioxidant defense, maintenance of photosynthetic systems, protection of reproductive tissues, regulation of flowering time, and miRNA biogenesis. In this review, we summarize the information acquired to date about the significance of plant miRNAs and their target genes in heat stress tolerance, thereby helping to identify the regulatory mechanisms that underlie heat stress responses in plants.", "gemini": "Căng thẳng do nhiệt là một căng thẳng phi sinh học chính ảnh hưởng đáng kể đến sự tăng trưởng và năng suất của cây trồng. Tuy nhiên, thực vật đã tiến hóa các cơ chế thích ứng phức tạp để đối phó với căng thẳng do nhiệt. MicroRNA (miRNA) là những phân tử quan trọng điều chỉnh biểu hiện gen thông qua quá trình phân hủy sau phiên mã các phân tử mRNA đích hoặc bằng cách kìm hãm quá trình dịch mã. miRNA của thực vật đóng vai trò thiết yếu trong quá trình phát triển và nhiều phản ứng căng thẳng khác nhau. Những tiến bộ gần đây trong công nghệ giải trình tự thông lượng cao đã giúp xác định và mô tả đặc điểm của ngày càng nhiều miRNA phản ứng với nhiệt ở nhiều loài thực vật khác nhau. Các miRNA được điều hòa bởi nhiệt kết hợp với gen đích của chúng tạo thành các mạng điều hòa lớn kiểm soát các con đường trao đổi chất khác nhau, bao gồm tái cấu trúc protein, chống oxy hóa, duy trì hệ thống quang hợp, bảo vệ mô sinh sản, điều hòa thời gian ra hoa và sinh tổng hợp miRNA. Trong bài tổng quan này, chúng tôi tóm tắt thông tin thu được cho đến nay về ý nghĩa của miRNA thực vật và gen đích của chúng trong khả năng chịu nhiệt, qua đó giúp xác định các cơ chế điều hòa cơ bản trong phản ứng chịu nhiệt ở thực vật."} +{"idx": 1870777, "en": "Equations of state (EoS) for fluids have been a staple of engineering design and practice for over a century. Available EoS are based on the fitting of a closed-form analytical expression to suitable experimental data. The mathematical structure and the underlying physical model significantly restrain the applicability and accuracy of the resulting EoS. This contribution explores the issues surrounding the substitution of machine-learned models for analytical EoS. In particular, we describe, as a proof of concept, the effectiveness of a machine-learned model to replicate the statistical associating fluid theory (SAFT-VR Mie) EoS for pure fluids. To quantify the effectiveness of machine-learning techniques, a large set of pseudodata is obtained from the EoS and used to train the machine-learning models. We employ artificial neural networks and Gaussian process regression to correlate and predict thermodynamic properties such as critical pressure and temperature, vapor pressures, and densities of pure model fluids; these are performed on the basis of molecular descriptors. The comparisons between the machine-learned EoS and the surrogate data set suggest that the proposed approach shows promise as a viable technique for the correlation and prediction of thermophysical properties of fluids.", "gemini": "Phương trình trạng thái (EoS) cho chất lỏng đã trở thành yếu tố chính trong thiết kế và thực hành kỹ thuật trong hơn một thế kỷ. Các EoS hiện có dựa trên việc lắp một biểu thức phân tích dạng đóng vào dữ liệu thực nghiệm phù hợp. Cấu trúc toán học và mô hình vật lý cơ bản hạn chế đáng kể khả năng áp dụng và độ chính xác của EoS thu được. Đóng góp này khám phá các vấn đề xung quanh việc thay thế các mô hình EoS phân tích bằng các mô hình học máy. Đặc biệt, chúng tôi mô tả, như một bằng chứng về khái niệm, hiệu quả của một mô hình học máy trong việc sao chép lý thuyết chất lỏng liên kết thống kê (SAFT-VR Mie) EoS cho chất lỏng tinh khiết. Để định lượng hiệu quả của các kỹ thuật học máy, một tập hợp lớn dữ liệu giả được lấy từ EoS và được sử dụng để đào tạo các mô hình học máy. Chúng tôi sử dụng mạng nơ-ron nhân tạo và hồi quy quá trình Gauss để tương quan và dự đoán các tính chất nhiệt động lực học như áp suất và nhiệt độ tới hạn, áp suất hơi và mật độ của chất lỏng mô hình tinh khiết; những điều này được thực hiện trên cơ sở các mô tả phân tử. Các so sánh giữa EoS học máy và bộ dữ liệu thay thế cho thấy phương pháp tiếp cận được đề xuất có triển vọng như một kỹ thuật khả thi để tương quan và dự đoán các tính chất nhiệt vật lý của chất lỏng."} +{"idx": 1870778, "en": "The subset of the proteome that contains enzymes in their catalytically active form can be interrogated by using probes targeted toward individual specific enzymes. A subset of such enzymes are proteases that are frequently studied with activity-based probes, small inhibitors equipped with a detectable tag, commonly a fluorophore. Due to the spectral overlap of these commonly used fluorophores, multiplex analysis becomes limited. To overcome this, we developed a series of protease-selective lanthanide-labeled probes compatible with mass cytometry giving us the ability to monitor the activity of multiple proteases in parallel. Using these probes, we were able to identify the distribution of four proteases with different active site geometries in three cell lines and peripheral blood mononuclear cells. This provides a framework for the use of mass cytometry for multiplexed enzyme activity detection.", "gemini": "Có thể thẩm vấn tập hợp con của proteome chứa các enzyme ở dạng hoạt động xúc tác của chúng bằng cách sử dụng các đầu dò nhắm vào từng enzyme cụ thể. Một tập hợp con của các enzyme như vậy là protease thường được nghiên cứu bằng các đầu dò dựa trên hoạt động, các chất ức chế nhỏ được trang bị thẻ có thể phát hiện được, thường là một chất huỳnh quang. Do phổ chồng chéo của các chất huỳnh quang thường được sử dụng này, nên việc phân tích đa kênh trở nên hạn chế. Để khắc phục điều này, chúng tôi đã phát triển một loạt các đầu dò được gắn lanthanide có chọn lọc protease tương thích với phép đo khối lượng tế bào cho chúng ta khả năng theo dõi hoạt động của nhiều protease song song. Sử dụng các đầu dò này, chúng tôi có thể xác định sự phân bố của bốn protease có hình dạng vị trí hoạt động khác nhau trong ba dòng tế bào và các tế bào đơn nhân máu ngoại vi. Điều này cung cấp một khuôn khổ cho việc sử dụng phép đo khối lượng tế bào để phát hiện hoạt động của nhiều enzyme."} +{"idx": 1870779, "en": "A crucial step in the gas-phase formation of ammonia in the interstellar medium (ISM) is the reaction of NH2+ with molecular hydrogen. Understanding the electronic structure of the participating species in this reaction and the evaluation of the rate coefficients at interstellar temperatures are, therefore, critical to gain new insights into the mechanisms of formation of interstellar ammonia. We present here the first theoretical results of the rate coefficients of this reaction as a function of temperatures relevant to the ISM, computed using transition-state theory. The results are in reasonable agreement with recent experimental data. This exothermic reaction features a tiny barrier which is primarily a consequence of zero-point energy corrections. The results demonstrate that quantum mechanical tunneling and core-electron correlations play significant roles in determining the rate of the reaction. The noteworthy failure of popular density functionals to describe this reaction is also highlighted.", "gemini": "Một bước quan trọng trong quá trình tạo thành amoniac ở pha khí trong môi trường giữa các vì sao (ISM) là phản ứng của NH2+ với hydro phân tử. Do đó, việc hiểu cấu trúc điện tử của các loài tham gia vào phản ứng này và đánh giá các hệ số tốc độ ở nhiệt độ giữa các vì sao là rất quan trọng để có được những hiểu biết mới về cơ chế hình thành amoniac giữa các vì sao. Chúng tôi trình bày ở đây những kết quả lý thuyết đầu tiên về hệ số tốc độ của phản ứng này như một hàm của nhiệt độ liên quan đến ISM, được tính toán bằng lý thuyết trạng thái chuyển tiếp. Kết quả phù hợp hợp lý với dữ liệu thực nghiệm gần đây. Phản ứng tỏa nhiệt này có một rào cản nhỏ chủ yếu là hệ quả của các hiệu chỉnh năng lượng điểm không. Kết quả chứng minh rằng sự chui hầm cơ học lượng tử và các tương quan electron lõi đóng vai trò quan trọng trong việc xác định tốc độ phản ứng. Sự thất bại đáng chú ý của các hàm mật độ phổ biến trong việc mô tả phản ứng này cũng được nêu bật."} +{"idx": 1870780, "en": "The Paternò-Büchi (PB) reaction is a common organic reaction in which a carbonyl radical formed by exposure to UV radiation reacts with an alkene to form an oxetane ring. Recent analytical applications of this reaction have included the determination of C═C bond position in lipid fatty acyl tails using tandem mass spectrometry. Our group has recently investigated methods for structurally modifying steroid isomers to improve their identification and resolution using ion mobility spectrometry. Herein, we report the first application of the Paternò-Büchi reaction to form steroid oxetanes using a simple, low-cost, and high efficiency method with a low pressure mercury lamp. This methodology is performed on several endogenous steroid isomers, resulting in unique ion mobility spectra that provide a unique fingerprint for each. These fingerprint spectra can add confidence in identification of those compounds, especially in complex biological matrixes. Testosterone and epitestosterone, an epimer pair commonly interrogated in a number of applications such as for their use as performance enhancing drugs, displayed one and three unique ion mobility peaks, respectively. These spectra and their measured collision cross sections (CCS) allow for unambiguous differentiation of these and several other steroid isomer groups analyzed in this work. Finally, multiple anabolic androgenic steroids prohibited by the World Anti-Doping Agency were tested with this method and resulted in unique CCS for their PB reaction products. This approach can offer improved confidence in their identification as well as for many other banned substances.", "gemini": "Phản ứng Paternò-Büchi (PB) là một phản ứng hữu cơ phổ biến trong đó gốc carbonyl được hình thành do tiếp xúc với bức xạ UV phản ứng với anken để tạo thành vòng oxetan. Các ứng dụng phân tích gần đây của phản ứng này bao gồm việc xác định vị trí liên kết C═C trong đuôi acyl béo lipid bằng phương pháp phổ khối song song. Nhóm của chúng tôi gần đây đã nghiên cứu các phương pháp để sửa đổi cấu trúc các đồng phân steroid nhằm cải thiện khả năng nhận dạng và độ phân giải của chúng bằng phương pháp phổ độ linh động ion. Trong bài viết này, chúng tôi báo cáo ứng dụng đầu tiên của phản ứng Paternò-Büchi để tạo thành các oxetan steroid bằng phương pháp đơn giản, chi phí thấp và hiệu suất cao với đèn thủy ngân áp suất thấp. Phương pháp này được thực hiện trên một số đồng phân steroid nội sinh, tạo ra phổ độ linh động ion độc đáo cung cấp dấu vân tay độc nhất cho từng đồng phân. Các phổ dấu vân tay này có thể tăng thêm độ tin cậy trong việc xác định các hợp chất đó, đặc biệt là trong các ma trận sinh học phức tạp. Testosterone và epitestosterone, một cặp epime thường được thẩm vấn trong một số ứng dụng như để sử dụng làm thuốc tăng cường hiệu suất, lần lượt hiển thị một và ba đỉnh độ linh động ion độc nhất. Các phổ này và mặt cắt va chạm được đo của chúng (CCS) cho phép phân biệt rõ ràng các nhóm đồng phân steroid này và một số nhóm đồng phân steroid khác được phân tích trong nghiên cứu này. Cuối cùng, nhiều steroid đồng hóa androgen bị Cơ quan chống doping thế giới cấm đã được thử nghiệm bằng phương pháp này và cho kết quả CCS độc nhất cho các sản phẩm phản ứng PB của chúng. Phương pháp tiếp cận này có thể cải thiện độ tin cậy trong việc xác định chúng cũng như nhiều chất bị cấm khác."} +{"idx": 1870781, "en": "Chemical modification of proteins in living cells permits valuable glimpses into the molecular interactions that underpin dynamic cellular events. While genetic engineering methods are often preferred, selective labeling of endogenous proteins in a complex intracellular milieu with chemical approaches represents a significant challenge. In this study, we report novel diazocoumarin compounds that can be photoactivated by visible (430-490 nm) and near-infrared light (800 nm) irradiation to photo-uncage reactive carbene intermediates, which could subsequently undergo an insertion reaction with concomitant fluorescence \"turned on\". With these new molecules in hand, we have developed a new approach for rapid, selective, and fluorogenic labeling of endogenous protein in living cells. By using CA-II and eDHFR as model proteins, we demonstrated that subcellular localization of proteins can be precisely visualized by live-cell imaging and protein levels can be reliably quantified in multiple cell types using flow cytometry. Dynamic protein regulations such as hypoxia-induced CA-IX accumulation can also be detected. In addition, by two-photon excitation with an 800 nm laser, cell-selective labeling can also be achieved with spatially controlled irradiation. Our method circumvents the cytotoxicity of UV light and obviates the need for introducing external reporters with \"click chemistries\". We believe that this approach of fluorescence labeling of endogenous protein by bioorthogonal photoirradiation opens up exciting opportunities for discoveries and mechanistic interrogation in chemical biology.", "gemini": "Biến đổi hóa học của protein trong các tế bào sống cho phép có được những cái nhìn thoáng qua có giá trị về các tương tác phân tử làm nền tảng cho các sự kiện tế bào động. Mặc dù các phương pháp kỹ thuật di truyền thường được ưa chuộng, nhưng việc đánh dấu chọn lọc các protein nội sinh trong môi trường nội bào phức tạp bằng các phương pháp tiếp cận hóa học vẫn là một thách thức đáng kể. Trong nghiên cứu này, chúng tôi báo cáo các hợp chất diazocoumarin mới có thể được hoạt hóa bằng bức xạ ánh sáng khả kiến (430-490 nm) và gần hồng ngoại (800 nm) để loại bỏ các trung gian cacben phản ứng, sau đó có thể trải qua phản ứng chèn với huỳnh quang đi kèm \"bật\". Với những phân tử mới này trong tay, chúng tôi đã phát triển một phương pháp tiếp cận mới để đánh dấu huỳnh quang nhanh chóng, chọn lọc và tạo huỳnh quang cho protein nội sinh trong các tế bào sống. Bằng cách sử dụng CA-II và eDHFR làm protein mô hình, chúng tôi đã chứng minh rằng có thể hình dung chính xác vị trí dưới tế bào của protein bằng hình ảnh tế bào sống và có thể định lượng đáng tin cậy mức protein trong nhiều loại tế bào bằng phương pháp đo lưu lượng tế bào. Các quy định về protein động như sự tích tụ CA-IX do thiếu oxy cũng có thể được phát hiện. Ngoài ra, bằng cách kích thích hai photon bằng laser 800 nm, cũng có thể đạt được việc đánh dấu chọn lọc tế bào với bức xạ được kiểm soát về mặt không gian. Phương pháp của chúng tôi khắc phục được độc tính tế bào của tia UV và loại bỏ nhu cầu đưa các chất báo cáo bên ngoài vào bằng \"phản ứng hóa học click\". Chúng tôi tin rằng phương pháp đánh dấu huỳnh quang protein nội sinh này bằng quang chiếu trực giao mở ra những cơ hội thú vị cho những khám phá và thẩm vấn cơ chế trong sinh học hóa học."} +{"idx": 1870782, "en": "On-lattice kinetic Monte Carlo (KMC) is a computational method used to simulate (among others) physicochemical processes on catalytic surfaces. The KMC algorithm propagates the system through discrete configurations by selecting (with the use of random numbers) the next elementary process to be simulated, e.g., adsorption, desorption, diffusion, or reaction. An implementation of such a selection procedure is the first-reaction method in which all realizable elementary processes are identified and assigned a random occurrence time based on their rate constant. The next event to be executed will then be the one with the minimum interarrival time. Thus, a fast and efficient algorithm for selecting the most imminent process and performing all of the necessary updates on the list of realizable processes post execution is of great importance. In the current work, we implement five data-structures to handle the elementary process queue during a KMC run: an unsorted list, a binary heap, a pairing heap, a one-way skip list, and finally, a novel two-way skip list with a mapping array specialized for KMC simulations. We also investigate the effect of compiler optimizations on the performance of these data-structures on three benchmark models, capturing CO oxidation, a simplified water gas shift mechanism, and a temperature-programmed desorption run. Excluding the least efficient and impractical for large-problems unsorted list, we observe a 3× speedup of the binary or pairing heaps (most efficient) compared to the one-way skip list (least efficient). Compiler optimizations deliver a speedup of up to 1.8×. These benchmarks provide valuable insight into the importance of, often-overlooked, implementation-related aspects of KMC simulations, such as the queueing data-structures. Our results could be particularly useful in guiding the choice of data-structures and algorithms that would minimize the computational cost of large-scale simulations.", "gemini": "Mô hình Monte Carlo động học trên mạng (KMC) là một phương pháp tính toán được sử dụng để mô phỏng (trong số những phương pháp khác) các quá trình vật lý hóa học trên bề mặt xúc tác. Thuật toán KMC truyền bá hệ thống thông qua các cấu hình rời rạc bằng cách chọn (với việc sử dụng các số ngẫu nhiên) quá trình cơ bản tiếp theo được mô phỏng, ví dụ như hấp phụ, giải hấp, khuếch tán hoặc phản ứng. Một triển khai của một quy trình lựa chọn như vậy là phương pháp phản ứng đầu tiên trong đó tất cả các quá trình cơ bản khả thi được xác định và được chỉ định một thời gian xảy ra ngẫu nhiên dựa trên hằng số tốc độ của chúng. Sự kiện tiếp theo được thực hiện sau đó sẽ là sự kiện có thời gian giữa các lần đến nhỏ nhất. Do đó, một thuật toán nhanh và hiệu quả để chọn quá trình sắp xảy ra nhất và thực hiện tất cả các cập nhật cần thiết trên danh sách các quá trình khả thi sau khi thực hiện là rất quan trọng. Trong công trình hiện tại, chúng tôi triển khai năm cấu trúc dữ liệu để xử lý hàng đợi quá trình cơ bản trong quá trình chạy KMC: một danh sách chưa được sắp xếp, một đống nhị phân, một đống ghép nối, một danh sách bỏ qua một chiều và cuối cùng là một danh sách bỏ qua hai chiều mới với một mảng ánh xạ chuyên dụng cho các phép mô phỏng KMC. Chúng tôi cũng nghiên cứu tác động của các tối ưu hóa trình biên dịch đối với hiệu suất của các cấu trúc dữ liệu này trên ba mô hình chuẩn, ghi lại quá trình oxy hóa CO, cơ chế dịch chuyển khí nước được đơn giản hóa và quá trình giải hấp được lập trình theo nhiệt độ. Ngoại trừ danh sách chưa được sắp xếp kém hiệu quả nhất và không thực tế cho các vấn đề lớn, chúng tôi quan sát thấy đống nhị phân hoặc đống ghép nối nhanh hơn 3 lần (hiệu quả nhất) so với danh sách bỏ qua một chiều (kém hiệu quả nhất). Các tối ưu hóa trình biên dịch cung cấp tốc độ nhanh hơn tới 1,8 lần. Các chuẩn này cung cấp cái nhìn sâu sắc có giá trị về tầm quan trọng của các khía cạnh liên quan đến việc triển khai thường bị bỏ qua của các phép mô phỏng KMC, chẳng hạn như các cấu trúc dữ liệu xếp hàng. Kết quả của chúng tôi có thể đặc biệt hữu ích trong việc hướng dẫn lựa chọn các cấu trúc dữ liệu và thuật toán sẽ giảm thiểu chi phí tính toán của các phép mô phỏng quy mô lớn."} +{"idx": 1870783, "en": "The water hexamer has many low-lying isomers, e.g., ring, book, cage, and prism, shifting from two- to three-dimensional structures. We show that this dimensionality change is accompanied by a drop in the quantum nature of the cluster, as manifested in the red shift of the quantal OH stretching modes as compared with their classical counterparts. We obtain this \"nuclear quantum effect\" (NQE) as the mean deviation between the OH stretch frequencies from velocity autocorrelation Fourier transforms from classical trajectories on a high-level water potential (MB-pol) as compared with scaled harmonic frequencies from high-level quantum chemistry calculations. With a universal scaling factor, the predicted OH frequencies agree with experiment to a mean absolute deviation ≤10 cm-1, which allows unequivocal isomer assignments. By assuming temperature-independent NQEs, we produce the temperature dependence of the cage isomer OH stretch spectrum below 70 K, where it is the dominant structure. All bands widen and blue-shift with increasing temperature, most conspicuously the reddest mode, which thus constitutes a \"vibrational thermometer\".", "gemini": "Hexame nước có nhiều đồng phân nằm thấp, ví dụ, vòng, sách, lồng và lăng trụ, thay đổi từ cấu trúc hai đến ba chiều. Chúng tôi cho thấy sự thay đổi chiều hướng này đi kèm với sự giảm bản chất lượng tử của cụm, như biểu hiện ở sự dịch chuyển đỏ của các chế độ kéo giãn OH lượng tử khi so sánh với các chế độ cổ điển tương ứng. Chúng tôi thu được \"hiệu ứng lượng tử hạt nhân\" (NQE) này dưới dạng độ lệch trung bình giữa các tần số kéo giãn OH từ phép biến đổi Fourier tự tương quan vận tốc từ các quỹ đạo cổ điển trên tiềm năng nước cấp cao (MB-pol) khi so sánh với tần số điều hòa được chia tỷ lệ từ các phép tính hóa học lượng tử cấp cao. Với một hệ số tỷ lệ phổ quát, các tần số OH dự đoán phù hợp với thí nghiệm với độ lệch tuyệt đối trung bình ≤10 cm-1, cho phép chỉ định đồng phân một cách rõ ràng. Bằng cách giả định các NQE không phụ thuộc vào nhiệt độ, chúng tôi tạo ra sự phụ thuộc vào nhiệt độ của phổ kéo giãn OH đồng phân lồng dưới 70 K, nơi nó là cấu trúc chiếm ưu thế. Tất cả các dải đều rộng ra và dịch chuyển xanh khi nhiệt độ tăng, rõ ràng nhất là chế độ đỏ nhất, do đó tạo thành một \"nhiệt kế rung\"."} +{"idx": 1870784, "en": "The antisymmetrized geminal power (AGP) wave function has a long history and is known by different names in various chemical and physical problems. There has been recent interest in using AGP as a starting point for strongly correlated electrons. Here, we show that in a seniority-conserving regime, different AGP-based correlator representations based on generators of the algebra, killing operators, and geminal replacement operators are all equivalent. We implement one representation that uses number operators as correlators and has linearly independent curvilinear metrics to distinguish the regions of Hilbert space. This correlation method called J-CI provides excellent accuracy in energies when applied to the pairing Hamiltonian.", "gemini": "Hàm sóng sức mạnh geminal phản đối xứng (AGP) có lịch sử lâu đời và được biết đến với nhiều tên khác nhau trong các vấn đề hóa học và vật lý khác nhau. Gần đây, người ta quan tâm đến việc sử dụng AGP làm điểm khởi đầu cho các electron có liên quan mạnh mẽ. Ở đây, chúng tôi chỉ ra rằng trong chế độ bảo toàn thâm niên, các biểu diễn tương quan dựa trên AGP khác nhau dựa trên các máy phát của đại số, các toán tử tiêu diệt và các toán tử thay thế geminal đều tương đương nhau. Chúng tôi triển khai một biểu diễn sử dụng các toán tử số làm tương quan và có các số liệu cong tuyến tính độc lập để phân biệt các vùng của không gian Hilbert. Phương pháp tương quan này được gọi là J-CI cung cấp độ chính xác tuyệt vời về năng lượng khi áp dụng cho Hamiltonian ghép đôi."} +{"idx": 1870785, "en": "Animal protein supplement feeds (APFs) are important raw feed materials for livestock. APFs might be susceptible to organophosphate esters (OPEs) but have not been paid attention yet. In the present study, animal-derived (meat meal, feather meal, and blood meal) and plant-derived APFs were all found to contain detectable levels of OPEs, with 16 target OPEs ranging from 12.6 ng/g dry weight (dw) to 301 ng/g dw. Meat meal contained the highest OPE level (mean: 117 ± 75.6 ng/g dw), followed by feather meal (54.6 ± 30.0 ng/g dw), plant-derived feed (41.9 ± 16.0 ng/g dw), and blood meal (28.0 ± 12.0 ng/g dw). Considering its widespread consumption, plant-derived APFs might be an important source of OPE exposure for livestock. Dust adhesion contributed to OPE contamination both in feather meal and plant-derived APFs. Congener patterns varied among the different APFs. Tris(2-chloroisopropyl) phosphate dominated in the plant-derived feed and blood meal, while tris(2-chloroethyl) phosphate and triphenyl phosphate were the major contributors in meat meal and feather meal, respectively. Tributyl phosphate and tri-iso-butyl phosphate were found to be statistically correlated in all APFs (p < 0.01), indicating their similar behavior and common sources. The protein-associated transport pathways of OPEs need to be studied separately for different protein matrices in the future.", "gemini": "Thức ăn bổ sung protein động vật (APF) là nguyên liệu thức ăn thô quan trọng cho gia súc. APF có thể dễ bị este organophosphate (OPE) nhưng vẫn chưa được chú ý. Trong nghiên cứu hiện tại, APF có nguồn gốc từ động vật (bột thịt, bột lông vũ và bột máu) và APF có nguồn gốc từ thực vật đều được phát hiện có chứa mức OPE có thể phát hiện được, với 16 mục tiêu OPE trong khoảng từ 12,6 ng/g trọng lượng khô (dw) đến 301 ng/g dw. Bột thịt chứa hàm lượng OPE cao nhất (trung bình: 117 ± 75,6 ng/g dw), tiếp theo là bột lông vũ (54,6 ± 30,0 ng/g dw), thức ăn có nguồn gốc từ thực vật (41,9 ± 16,0 ng/g dw) và bột máu (28,0 ± 12,0 ng/g dw). Xem xét mức tiêu thụ rộng rãi của nó, APF có nguồn gốc từ thực vật có thể là một nguồn phơi nhiễm OPE quan trọng đối với gia súc. Bụi bám góp phần gây ô nhiễm OPE trong cả bột lông vũ và APF có nguồn gốc từ thực vật. Các kiểu đồng loại khác nhau giữa các APF khác nhau. Tris(2-chloroisopropyl) phosphate chiếm ưu thế trong thức ăn có nguồn gốc từ thực vật và bột máu, trong khi tris(2-chloroethyl) phosphate và triphenyl phosphate lần lượt là những tác nhân chính trong bột thịt và bột lông vũ. Tributyl phosphate và tri-iso-butyl phosphate được phát hiện có mối tương quan thống kê trong tất cả các APF (p < 0,01), chỉ ra hành vi tương tự và nguồn gốc chung của chúng. Các con đường vận chuyển OPE liên quan đến protein cần được nghiên cứu riêng biệt cho các ma trận protein khác nhau trong tương lai."} +{"idx": 1870786, "en": "This work aimed to predict C18:1 TFA isomers as well as other groups of fatty acids (saturated, monounsaturated, polyunsaturated, and total TFA) in chocolates by ATR-FTIR and partial least square regression. The quantification of fatty acids in representative samples (white, dark, and milk chocolates) was rapid (<30 s) and did not require derivatization. The optimized models showed satisfactory linear correlations compared to a reference gas chromatographic method. Coefficients of correlation for prediction considering C18:1 positional isomers were 0.973 (trans 6-8), 0.991 (trans 9), 0.991 (trans 10), 0.988 (trans 11), and 0.998 (trans 12). When considering fatty acids groups, these coefficients ranged from 0.965 to 0.999. The obtained results indicate that this straightforward procedure is suitable for chocolate analysis, for determining its general lipid composition and TFA isomeric profile, which would be of great interest for quality control programs in the face of the new TFA regulations.", "gemini": "Nghiên cứu này nhằm mục đích dự đoán các đồng phân C18:1 TFA cũng như các nhóm axit béo khác (bão hòa, không bão hòa đơn, không bão hòa đa và tổng TFA) trong sô cô la bằng ATR-FTIR và hồi quy bình phương cực tiểu từng phần. Việc định lượng các axit béo trong các mẫu đại diện (sô cô la trắng, đen và sữa) diễn ra nhanh chóng (<30 giây) và không yêu cầu dẫn xuất hóa. Các mô hình được tối ưu hóa cho thấy mối tương quan tuyến tính đáng kể so với phương pháp sắc ký khí tham chiếu. Hệ số tương quan để dự đoán khi xem xét các đồng phân vị trí C18:1 là 0,973 (trans 6-8), 0,991 (trans 9), 0,991 (trans 10), 0,988 (trans 11) và 0,998 (trans 12). Khi xem xét các nhóm axit béo, các hệ số này nằm trong khoảng từ 0,965 đến 0,999. Các kết quả thu được cho thấy quy trình đơn giản này phù hợp để phân tích sô cô la, để xác định thành phần lipid chung và cấu hình đồng phân TFA của sô cô la, điều này sẽ rất được quan tâm đối với các chương trình kiểm soát chất lượng trong bối cảnh các quy định mới về TFA."} +{"idx": 1870787, "en": "Membrane solubilization by sodium dodecyl sulfate (SDS) is indispensable for many established biotechnological applications, including viral inactivation and protein extraction. Although the ensemble thermodynamics have been thoroughly explored, the underlying molecular dynamics have remained inaccessible, owing to major limitations of traditional measurement tools. Here, we integrate multiple advanced biophysical approaches to gain multiangle insight into the time-dependence and fundamental kinetic steps associated with the solubilization of single submicron sized vesicles in response to SDS. We find that the accumulation of SDS molecules on intact vesicles triggers biphasic solubilization kinetics comprising an initial vesicle expansion event followed by rapid lipid loss and micellization. Our findings support a general mechanism of detergent-induced membrane solubilization, and we expect that the framework of correlative biophysical technologies presented here will form a general platform for elucidating the complex kinetics of membrane perturbation induced by a wide variety of surfactants and disrupting agents.", "gemini": "Việc hòa tan màng bằng natri dodecyl sulfate (SDS) là điều bắt buộc đối với nhiều ứng dụng công nghệ sinh học đã được thiết lập, bao gồm cả việc bất hoạt vi-rút và chiết xuất protein. Mặc dù nhiệt động lực học của hệ số đã được khám phá kỹ lưỡng, nhưng động lực học phân tử cơ bản vẫn chưa thể tiếp cận được do những hạn chế lớn của các công cụ đo lường truyền thống. Tại đây, chúng tôi tích hợp nhiều phương pháp sinh học vật lý tiên tiến để có được cái nhìn sâu sắc đa góc độ về sự phụ thuộc thời gian và các bước động học cơ bản liên quan đến việc hòa tan các túi có kích thước dưới micron đơn lẻ để đáp ứng với SDS. Chúng tôi thấy rằng sự tích tụ của các phân tử SDS trên các túi còn nguyên vẹn gây ra động học hòa tan hai pha bao gồm sự kiện mở rộng túi ban đầu theo sau là mất lipid và micelle hóa nhanh chóng. Những phát hiện của chúng tôi hỗ trợ một cơ chế chung về quá trình hòa tan màng do chất tẩy rửa gây ra và chúng tôi hy vọng rằng khuôn khổ các công nghệ sinh học vật lý tương quan được trình bày ở đây sẽ tạo thành một nền tảng chung để làm sáng tỏ động học phức tạp của sự nhiễu loạn màng do nhiều loại chất hoạt động bề mặt và tác nhân phá vỡ gây ra."} +{"idx": 1870788, "en": "Cesium copper halides (CCHs) show promise for optoelectronic applications, and their syntheses usually involve high-temperatures and hazard solvents. Herein, the synthesis of highly luminescent and phase-pure Cs3Cu2X5 (X = Cl, Br, and I) and CsCu2I3 via a solvent-free mechanochemical approach through manual grinding is demonstrated. This cost-effective approach can produce CCHs on a scale of tens to hundreds of grams. Rietveld refinement analysis of the X-ray diffraction patterns of the as-synthesized CCHs reveals their structural details. Notably, the emission characteristics of green-emitting, chloride-based CCHs remain stable even at elevated temperatures-maintaining 80% of initial PL efficiency at 150 °C. Lastly, a postsynthetic reversible transformation between zero- and one-dimensional CCH materials is demonstrated, indicating the labile nature of their crystal structure. The proposed study suggests that mechanochemistry can be an alternative and promising synthetic tool for fabricating high-quality lead-free metal halides.", "gemini": "Các halide đồng cesium (CCH) cho thấy triển vọng cho các ứng dụng quang điện tử và quá trình tổng hợp của chúng thường liên quan đến nhiệt độ cao và dung môi nguy hiểm. Trong nghiên cứu này, quá trình tổng hợp Cs3Cu2X5 (X = Cl, Br và I) và CsCu2I3 có độ phát quang cao và pha tinh khiết thông qua phương pháp cơ học không dung môi thông qua nghiền thủ công đã được chứng minh. Phương pháp tiết kiệm chi phí này có thể sản xuất CCH với quy mô từ hàng chục đến hàng trăm gam. Phân tích tinh chỉnh Rietveld đối với các mẫu nhiễu xạ tia X của CCH tổng hợp cho thấy các chi tiết cấu trúc của chúng. Đáng chú ý, các đặc tính phát xạ của CCH gốc clorua phát ra màu xanh lục vẫn ổn định ngay cả ở nhiệt độ cao - duy trì 80% hiệu suất PL ban đầu ở 150 °C. Cuối cùng, một quá trình chuyển đổi thuận nghịch sau tổng hợp giữa các vật liệu CCH một chiều và không chiều được chứng minh, cho thấy bản chất không bền của cấu trúc tinh thể của chúng. Nghiên cứu được đề xuất cho thấy cơ học có thể là một công cụ tổng hợp thay thế và đầy hứa hẹn để chế tạo các halide kim loại không chì chất lượng cao."} +{"idx": 1870789, "en": "One of the most important advantages of mass spectrometry is the ability to quantify proteins and their modifications in parallel to obtain a holistic picture of the protein of interest. Here, we present a hybrid immunoaffinity targeted mass spectrometry (MS) method that combines efficient pan-antibody enrichment of a specific protein from plasma with the selectivity of high-resolution targeted MS analysis to quantitate specific proteoforms of interest. We used this approach to quantify plasma levels of the chemokine CXCL10 that has been associated with many immunological disorders such as systemic lupus erythematosus and primary Sjögren's Syndrome (pSS). The hybrid approach enabled sensitive, specific, and simultaneous quantification of total, full-length (active) CXCL101-77 and DPP4-truncated (inactive) CXCL103-77 in human plasma down to the low pg/mL level, reaching ELISA sensitivities. Samples from 30 control subjects and 34 pSS patients (n = 64) were analyzed. The ratio of CXCL101-77 to truncated CXCL103-77 was significantly increased in patients with pSS and provided the highest correlation with pSS disease activity. Therefore, this CXCL10 proteoform ratio represents an interesting exploratory disease activity biomarker to further investigate. As this strategy can be readily adapted to other plasma proteins and proteoforms of interest, we are convinced that it will lead to a more detailed understanding of proteoforms in physiology and pathology yielding more relevant biomarkers and drug targets.", "gemini": "Một trong những ưu điểm quan trọng nhất của phổ khối là khả năng định lượng protein và các biến đổi của chúng song song để có được bức tranh toàn diện về protein được quan tâm. Tại đây, chúng tôi trình bày phương pháp phổ khối (MS) nhắm mục tiêu miễn dịch ái lực lai kết hợp làm giàu kháng thể toàn bộ hiệu quả của một protein cụ thể từ huyết tương với tính chọn lọc của phân tích MS nhắm mục tiêu có độ phân giải cao để định lượng các dạng protein cụ thể được quan tâm. Chúng tôi đã sử dụng phương pháp tiếp cận này để định lượng nồng độ chemokine CXCL10 trong huyết tương có liên quan đến nhiều rối loạn miễn dịch như lupus ban đỏ hệ thống và hội chứng Sjögren nguyên phát (pSS). Phương pháp tiếp cận lai cho phép định lượng toàn bộ, toàn bộ chiều dài (hoạt động) CXCL101-77 và CXCL103-77 bị cắt cụt DPP4 (không hoạt động) trong huyết tương người xuống mức pg/mL thấp, đạt độ nhạy của ELISA. Các mẫu từ 30 đối tượng đối chứng và 34 bệnh nhân pSS (n = 64) đã được phân tích. Tỷ lệ CXCL101-77 so với CXCL103-77 bị cắt cụt tăng đáng kể ở những bệnh nhân mắc pSS và có mối tương quan cao nhất với hoạt động của bệnh pSS. Do đó, tỷ lệ dạng protein CXCL10 này đại diện cho một dấu ấn hoạt động của bệnh khám phá thú vị để tiếp tục nghiên cứu. Vì chiến lược này có thể dễ dàng áp dụng cho các protein huyết tương khác và các dạng protein được quan tâm, chúng tôi tin rằng nó sẽ dẫn đến sự hiểu biết chi tiết hơn về các dạng protein trong sinh lý và bệnh lý học, tạo ra nhiều dấu ấn sinh học và mục tiêu thuốc có liên quan hơn."} +{"idx": 1870790, "en": "Using muon-spin spectroscopy, we study the exceptional dynamical properties of rotating molecular struts engineered within a Zn-based metal-organic framework at cryogenic temperatures, where the internal motions of almost any other organic substance are quenched. Muon-spin spectroscopy is particularly suited for this aim, as the experimental evidence suggests several implantation sites for the muons, among which at least one directly onto the rotating moiety. The dynamics of the molecular rotors are characterized by the exceptionally low activation energy EA ∼ 30 cal mol-1. At the same time, we evidence a highly unusual temperature dependence of the dipolar interaction of muons with nuclear magnetic moments on the rotors, suggesting a complex influence of the rotations on the muon implantation and diffusion.", "gemini": "Sử dụng phổ học muon-spin, chúng tôi nghiên cứu các đặc tính động lực học đặc biệt của các thanh chống phân tử quay được thiết kế trong một khung kim loại hữu cơ gốc Zn ở nhiệt độ lạnh, nơi các chuyển động bên trong của hầu hết mọi chất hữu cơ khác đều bị dập tắt. Phổ học muon-spin đặc biệt phù hợp với mục đích này, vì bằng chứng thực nghiệm cho thấy một số vị trí cấy ghép cho các muon, trong đó có ít nhất một vị trí trực tiếp vào phần quay. Động lực học của các rôto phân tử được đặc trưng bởi năng lượng hoạt hóa cực thấp EA ∼ 30 cal mol-1. Đồng thời, chúng tôi chứng minh sự phụ thuộc nhiệt độ rất bất thường của tương tác lưỡng cực của các muon với các mômen từ hạt nhân trên các rôto, cho thấy ảnh hưởng phức tạp của các vòng quay lên sự cấy ghép và khuếch tán của muon."} +{"idx": 1870791, "en": "Using fixed dose combinations of drugs instead of administering drugs separately can be beneficial for both patients and the health care system, but the current understanding of how multidrug formulations work at the molecular level is still in its infancy. Here, we explore dissolution, solubility, and supersaturation of various drug combinations in amorphous formulations. The effect of chemical structural similarity on combination behavior was investigated by using structurally related compounds of both drugs. The effect of polymer type on solution behavior was also evaluated using chemically diverse polymers. Indapamide (IPM) concentration decreased when combined with felodipine (FDN) or its analogues, which occurred even when the IPM solution was undersaturated. The extent of solubility decrease of FDN was less than that of IPM from the dissolution of an equimolar formulation of the drugs. No significant solubility decrease was observed for FDN at low contents of IPM which was also observed for other dihydropyridines, whereas FDN decreases at high contents of IPM. This was explained by the complex nature of the colloidal precipitates of the combinations which impacts the chemical potential of the drugs in solution at different levels. The maximum achievable concentration of FDN and IPM during dissolution of the polyvinylpyrrolidone-based amorphous solid dispersion was higher than the value measured with the hydroxypropyl methylcellulose acetate succinate-based formulation. This emphasizes the significance of molecular properties and chemical diversity of drugs and polymers on solution chemistry and solubility profiles. These findings may apply to drugs administered as a single dosage form or in separate dosage forms and hence need to be well controlled to assure effective treatments and patient safety.", "gemini": "Sử dụng các kết hợp liều cố định của thuốc thay vì dùng thuốc riêng biệt có thể có lợi cho cả bệnh nhân và hệ thống chăm sóc sức khỏe, nhưng sự hiểu biết hiện tại về cách các công thức đa thuốc hoạt động ở cấp độ phân tử vẫn còn trong giai đoạn trứng nước. Tại đây, chúng tôi khám phá độ hòa tan, độ hòa tan và độ quá bão hòa của nhiều kết hợp thuốc khác nhau trong các công thức vô định hình. Tác động của sự tương đồng về cấu trúc hóa học đối với hành vi kết hợp đã được nghiên cứu bằng cách sử dụng các hợp chất có liên quan về mặt cấu trúc của cả hai loại thuốc. Tác động của loại polyme đối với hành vi của dung dịch cũng được đánh giá bằng cách sử dụng các polyme có tính chất hóa học khác nhau. Nồng độ indapamide (IPM) giảm khi kết hợp với felodipine (FDN) hoặc các chất tương tự của nó, điều này xảy ra ngay cả khi dung dịch IPM chưa bão hòa. Mức độ giảm độ hòa tan của FDN thấp hơn so với IPM khi hòa tan một công thức thuốc có tỷ lệ mol bằng nhau. Không thấy giảm độ hòa tan đáng kể đối với FDN ở hàm lượng IPM thấp, điều này cũng được quan sát thấy đối với các dihydropyridine khác, trong khi FDN giảm ở hàm lượng IPM cao. Điều này được giải thích bởi bản chất phức tạp của các kết tủa keo của các kết hợp, ảnh hưởng đến thế hóa học của thuốc trong dung dịch ở các mức độ khác nhau. Nồng độ tối đa có thể đạt được của FDN và IPM trong quá trình hòa tan của phân tán rắn vô định hình gốc polyvinylpyrrolidone cao hơn giá trị đo được với công thức gốc hydroxypropyl methylcellulose acetate succinate. Điều này nhấn mạnh tầm quan trọng của các tính chất phân tử và sự đa dạng về mặt hóa học của thuốc và polyme đối với hóa học dung dịch và hồ sơ độ hòa tan. Những phát hiện này có thể áp dụng cho các loại thuốc được dùng dưới dạng một dạng bào chế đơn lẻ hoặc ở các dạng bào chế riêng biệt và do đó cần được kiểm soát tốt để đảm bảo hiệu quả điều trị và an toàn cho bệnh nhân."} +{"idx": 1870792, "en": "Leveraging recent advances in electron energy monochromation and aberration correction, we record the spatially resolved infrared plasmon spectrum of individual tin-doped indium oxide nanocrystals using electron energy-loss spectroscopy (EELS). Both surface and bulk plasmon responses are measured as a function of tin doping concentration from 1-10 atomic percent. These results are compared to theoretical models, which elucidate the spectral detuning of the same surface plasmon resonance feature when measured from aloof and penetrating probe geometries. We additionally demonstrate a unique approach to retrieving the fundamental dielectric parameters of individual semiconductor nanocrystals via EELS. This method, devoid from ensemble averaging, illustrates the potential for electron-beam ellipsometry measurements on materials that cannot be prepared in bulk form or as thin films.", "gemini": "Tận dụng những tiến bộ gần đây trong phép đơn sắc năng lượng electron và hiệu chỉnh quang sai, chúng tôi ghi lại phổ plasmon hồng ngoại được giải quyết không gian của các tinh thể nano oxit indi được pha tạp thiếc riêng lẻ bằng cách sử dụng phổ học mất năng lượng electron (EELS). Cả phản ứng plasmon bề mặt và khối đều được đo như một hàm của nồng độ pha tạp thiếc từ 1-10 phần trăm nguyên tử. Những kết quả này được so sánh với các mô hình lý thuyết, làm sáng tỏ sự sai lệch phổ của cùng một đặc điểm cộng hưởng plasmon bề mặt khi đo từ các hình học đầu dò xuyên thấu và xa. Ngoài ra, chúng tôi còn chứng minh một cách tiếp cận độc đáo để lấy lại các thông số điện môi cơ bản của các tinh thể nano bán dẫn riêng lẻ thông qua EELS. Phương pháp này, không có trung bình tổng thể, minh họa tiềm năng cho các phép đo ellipsometry chùm tia electron trên các vật liệu không thể chế tạo ở dạng khối hoặc dạng màng mỏng."} +{"idx": 1870793, "en": "Metal-organic frameworks (MOFs) can provide exceptional porosity for molecular guest encapsulation useful for emergent applications in sensing, gas storage, drug delivery, and optoelectronics. Central to the realization of such applications, however, is the successful incorporation of a functional guest confined within the host framework. Here, we demonstrate, for the first time, the feasibility of scattering-type scanning near-field optical microscopy (s-SNOM) and nano-Fourier transform infrared (nanoFTIR) spectroscopy, in concert with density functional theory (DFT) calculations to reveal the vibrational characteristics of the Guest@MOF systems. Probing individual MOF crystals, we pinpoint the local molecular vibrations and, thus, shed new light on the host-guest interactions at the nanoscale. Our strategy not only confirms the successful encapsulation of luminescent guest molecules in the porous host framework in single crystals but also further provides a new methodology for nanoscale-resolved physical and chemical identification of wide-ranging framework materials and designer porous systems for advanced applications.", "gemini": "Các khung kim loại-hữu cơ (MOF) có thể cung cấp độ xốp đặc biệt để đóng gói phân tử khách hữu ích cho các ứng dụng mới nổi trong cảm biến, lưu trữ khí, phân phối thuốc và quang điện tử. Tuy nhiên, cốt lõi để hiện thực hóa các ứng dụng như vậy là sự kết hợp thành công của một phân tử khách chức năng được giới hạn trong khung vật chủ. Ở đây, chúng tôi chứng minh, lần đầu tiên, tính khả thi của kính hiển vi quang học trường gần quét kiểu tán xạ (s-SNOM) và phổ hồng ngoại biến đổi Fourier nano (nanoFTIR), kết hợp với các tính toán lý thuyết hàm mật độ (DFT) để tiết lộ đặc điểm dao động của Hệ thống Guest@MOF. Đo các tinh thể MOF riêng lẻ, chúng tôi xác định chính xác các dao động phân tử cục bộ và do đó, làm sáng tỏ các tương tác vật chủ-khách ở cấp độ nano. Chiến lược của chúng tôi không chỉ xác nhận sự đóng gói thành công các phân tử khách phát quang trong khung vật chủ xốp ở các tinh thể đơn mà còn cung cấp thêm một phương pháp luận mới để xác định vật lý và hóa học ở cấp độ nano của các vật liệu khung rộng và các hệ thống xốp thiết kế cho các ứng dụng tiên tiến."} +{"idx": 1870794, "en": "We demonstrate that one-dimensional moiré patterns, analogous to those found in twisted bilayer graphene, can arise in collapsed chiral carbon nanotubes. Resorting to a combination of approaches, namely, molecular dynamics to obtain the relaxed geometries and tight-binding calculations validated against ab initio modeling, we find that magic angle physics occur in collapsed carbon nanotubes. Velocity reduction, flat bands, and localization in AA regions with diminishing moiré angle are revealed, showing a magic angle close to 1°. From the spatial extension of the AA regions and the width of the flat bands, we estimate that many-body interactions in these systems are stronger than in twisted bilayer graphene. Chiral collapsed carbon nanotubes stand out as promising candidates to explore many-body effects and superconductivity in low dimensions, emerging as the one-dimensional analogues of twisted bilayer graphene.", "gemini": "Chúng tôi chứng minh rằng các mẫu moiré một chiều, tương tự như các mẫu tìm thấy trong graphene hai lớp xoắn, có thể phát sinh trong các ống nano carbon không đối xứng bị sụp đổ. Áp dụng kết hợp các phương pháp, cụ thể là động lực học phân tử để thu được các hình học được nới lỏng và các tính toán liên kết chặt chẽ được xác thực so với mô hình ab initio, chúng tôi thấy rằng vật lý góc kỳ diệu xảy ra trong các ống nano carbon bị sụp đổ. Sự giảm tốc độ, các dải phẳng và định vị trong các vùng AA với góc moiré giảm dần được tiết lộ, cho thấy một góc kỳ diệu gần 1°. Từ phần mở rộng không gian của các vùng AA và chiều rộng của các dải phẳng, chúng tôi ước tính rằng các tương tác nhiều thân trong các hệ thống này mạnh hơn trong graphene hai lớp xoắn. Các ống nano carbon không đối xứng bị sụp đổ nổi bật như những ứng cử viên đầy hứa hẹn để khám phá các hiệu ứng nhiều thân và tính siêu dẫn ở các chiều thấp, nổi lên như các chất tương tự một chiều của graphene hai lớp xoắn."} +{"idx": 1870795, "en": "Kinetic parameters for the [5,6]- to [6,6]-[Li+@PCBM]TFSI- transformation were determined experimentally, revealing a ca. 700-fold faster reaction rate at 423 K than empty PCBM and a 57.4 kJ mol-1 lower activation energy. The encapsulated Li+ can be considered as solvation-free Li+, forming a 1:1 complex with the substrate.", "gemini": "Các thông số động học cho quá trình chuyển đổi [5,6] sang [6,6]-[Li+@PCBM]TFSI- đã được xác định bằng thực nghiệm, cho thấy tốc độ phản ứng nhanh hơn khoảng 700 lần ở 423 K so với PCBM trống và năng lượng hoạt hóa thấp hơn 57,4 kJ mol-1. Li+ được bao bọc có thể được coi là Li+ không hòa tan, tạo thành phức hợp 1:1 với chất nền."} +{"idx": 1870796, "en": "The purpose of surgically facilitated orthodontic treatment (SFOT) is to increase and thicken the periodontal biotype, including both hard and soft tissue, and facilitate accelerated tooth movement through the induction of the regional acceleratory phenomenon. This article discusses the surgical and biomaterial factors related to increased predictability of bone augmentation when performing this pre-orthodontic surgical technique. Critical surgery-related factors of SFOT that will be examined include incision and flap design and closure, the depth and location of corticotomies, surgical trauma, augmentation protocols, and postoperative considerations. Biomaterial-related factors that will be reviewed encompass bone grafting materials, biologics, barrier membranes, and planned tooth movement.", "gemini": "Mục đích của điều trị chỉnh nha được hỗ trợ bằng phẫu thuật (SFOT) là làm tăng và làm dày kiểu hình sinh học quanh răng, bao gồm cả mô cứng và mô mềm, đồng thời tạo điều kiện thuận lợi cho răng di chuyển nhanh hơn thông qua việc tạo ra hiện tượng tăng tốc cục bộ. Bài viết này thảo luận về các yếu tố phẫu thuật và sinh học liên quan đến khả năng dự đoán tăng sinh xương khi thực hiện kỹ thuật phẫu thuật chỉnh nha trước này. Các yếu tố quan trọng liên quan đến phẫu thuật của SFOT sẽ được xem xét bao gồm thiết kế và đóng mở vết rạch và vạt, độ sâu và vị trí của các đường cắt vỏ, chấn thương do phẫu thuật, các giao thức tăng sinh và các cân nhắc sau phẫu thuật. Các yếu tố liên quan đến sinh học sẽ được xem xét bao gồm vật liệu ghép xương, sinh học, màng chắn và kế hoạch di chuyển răng."} +{"idx": 1870797, "en": "In early 2020, a new health threat arose with symptoms and mode of transmission comparable to severe acute respiratory syndrome (SARS). Both SARS and the new disease, COVID-19, are caused by coronaviruses and share similar characteristics, especially their severity and high mortality rates and an apparently greater likelihood of transmission. Seasonal influenza is caused by numerous and changing groups of viruses and, for most people, is much less serious. The purpose of this article is to help clinicians compare seasonal influenza, SARS, and COVID-19. It discusses what constitutes best evidence in a time of pandemic, describing the hierarchy of evidence. It is intended to assist dental professionals in providing accurate, current information in answer to patients' questions regarding testing and treatment.", "gemini": "Đầu năm 2020, một mối đe dọa sức khỏe mới đã xuất hiện với các triệu chứng và cách thức lây truyền tương tự như hội chứng hô hấp cấp tính nặng (SARS). Cả SARS và căn bệnh mới, COVID-19, đều do virus corona gây ra và có những đặc điểm tương tự, đặc biệt là mức độ nghiêm trọng và tỷ lệ tử vong cao cùng khả năng lây truyền rõ ràng cao hơn. Cúm theo mùa do nhiều nhóm vi-rút khác nhau và thay đổi gây ra, và đối với hầu hết mọi người, bệnh này ít nghiêm trọng hơn nhiều. Mục đích của bài viết này là giúp các bác sĩ lâm sàng so sánh cúm theo mùa, SARS và COVID-19. Bài viết thảo luận về những gì cấu thành bằng chứng tốt nhất trong thời kỳ đại dịch, mô tả hệ thống phân cấp bằng chứng. Mục đích là hỗ trợ các chuyên gia nha khoa cung cấp thông tin chính xác, cập nhật để trả lời các câu hỏi của bệnh nhân liên quan đến xét nghiệm và điều trị."} +{"idx": 1870798, "en": "A gingival fenestration is a localized perforation of keratinized gingival tissue that may result in underlying bone resorption and root exposure. This case report describes treatment of mandibular lingual gingival fenestrations caused by traumatic forces from an ill-fitting removable partial denture that rested on a thin periodontal phenotype. A subepithelial connective tissue graft was used to correct these fenestrations. At the 2-year follow-up, the gingival tissues presented in a state of health.", "gemini": "Một lỗ thủng nướu răng là một lỗ thủng cục bộ trên mô nướu răng đã sừng hóa có thể dẫn đến tiêu xương bên dưới và lộ chân răng. Báo cáo trường hợp này mô tả cách điều trị lỗ thủng nướu răng lưỡi hàm dưới do lực chấn thương từ một hàm giả tháo lắp không vừa khít nằm trên kiểu hình nha chu mỏng. Một miếng ghép mô liên kết dưới biểu mô đã được sử dụng để khắc phục những lỗ thủng này. Sau 2 năm theo dõi, các mô nướu răng đã cải thiện sức khỏe."} +{"idx": 1870799, "en": "With the incidence of failed and ailing dental implants increasing, the authors conducted research to evaluate and characterize all known criteria used in the assessment of implant health in the clinical setting. A review of articles found in electronic databases was performed. Once all parameters for implant assessment as supported by current literature were selected, implant assessment documents were created: a data acquisition form and a quantitative comprehensive evaluation. These documents provide the clinician an algorithm that yields a prognosis of survival for each implant. Diagnostic criteria were organized to offer a comprehensive assessment of risk factors related to implant health. Data acquisition prior to establishing the prognosis is necessary in accordance with the staging system developed. Parameters for diagnosis and staging include a thorough medical/social history of the patient, implant history, and clinical evaluation. The authors concluded that the establishment of parameters for comprehensive implant evaluation in the clinical setting is feasible. This assessment process enables an effective clinical approach to evaluate and treat ailing implants while facilitating a clinical diagnostic algorithm.", "gemini": "Với tỷ lệ cấy ghép răng thất bại và hư hỏng ngày càng tăng, các tác giả đã tiến hành nghiên cứu để đánh giá và mô tả tất cả các tiêu chí đã biết được sử dụng trong việc đánh giá sức khỏe của cấy ghép trong môi trường lâm sàng. Đã thực hiện xem xét các bài báo được tìm thấy trong cơ sở dữ liệu điện tử. Sau khi tất cả các thông số để đánh giá cấy ghép được hỗ trợ bởi các tài liệu hiện tại đã được chọn, các tài liệu đánh giá cấy ghép đã được tạo: một biểu mẫu thu thập dữ liệu và một đánh giá toàn diện định lượng. Các tài liệu này cung cấp cho bác sĩ lâm sàng một thuật toán đưa ra tiên lượng sống sót cho từng cấy ghép. Các tiêu chuẩn chẩn đoán đã được sắp xếp để đưa ra đánh giá toàn diện về các yếu tố rủi ro liên quan đến sức khỏe của cấy ghép. Việc thu thập dữ liệu trước khi thiết lập tiên lượng là cần thiết theo hệ thống phân giai đã phát triển. Các thông số để chẩn đoán và phân giai bao gồm tiền sử y tế/xã hội của bệnh nhân, tiền sử cấy ghép và đánh giá lâm sàng. Các tác giả kết luận rằng việc thiết lập các thông số để đánh giá toàn diện cấy ghép trong môi trường lâm sàng là khả thi. Quy trình đánh giá này cho phép tiếp cận lâm sàng hiệu quả để đánh giá và điều trị các cấy ghép bị hư hỏng đồng thời tạo điều kiện thuận lợi cho thuật toán chẩn đoán lâm sàng."} +{"idx": 1870800, "en": "The treatment of incisor protrusion by means of tooth extraction can be challenging for orthodontists, especially during the space closure phase. Moreover, the level of difficulty may increase when anterior movement of the posterior teeth is not desirable. Treatment alternatives may include the use of mini implants, mini plates, and extraoral devices to reinforce anchorage; however, some patients may oppose these aggressive methods. This article describes the use of frictionless segmented mechanics that provide differential moments for controlled space closure during full retraction of the incisors without using extraoral forces or temporary anchorage devices.", "gemini": "Việc điều trị nhô răng cửa bằng cách nhổ răng có thể là một thách thức đối với các bác sĩ chỉnh nha, đặc biệt là trong giai đoạn đóng khoảng trống. Hơn nữa, mức độ khó có thể tăng lên khi không mong muốn răng sau di chuyển về phía trước. Các phương pháp điều trị thay thế có thể bao gồm sử dụng các trụ nhỏ, máng nhỏ và các thiết bị ngoài miệng để gia cố neo; tuy nhiên, một số bệnh nhân có thể phản đối các phương pháp xâm lấn này. Bài viết này mô tả việc sử dụng cơ học phân đoạn không ma sát cung cấp các mômen khác biệt để đóng khoảng trống có kiểm soát trong quá trình thu toàn bộ răng cửa mà không cần sử dụng lực ngoài miệng hoặc các thiết bị neo tạm thời."} +{"idx": 1870801, "en": "The replacement of missing teeth is more complex than simply placing a dental implant and restoring it, especially when a single implant site is involved. Implants have a round cross-section, whereas natural teeth do not but instead have crestal cross-sections that vary in shape from ovoid to triangular to rectangular depending on which tooth is being replaced. When this factor is combined with the zone between the crestal bone and adjacent proximal contacts, an emergence profile presents that will govern the esthetics of the restoration. The emergence profile will also guide and maintain the soft tissue, preventing potential food accumulation areas interproximally. This article defines an emergence profile and reviews the importance of establishing it and how to develop it based on the tooth being replaced to achieve natural-appearing restorations.", "gemini": "Việc thay thế răng mất phức tạp hơn là chỉ đặt một implant nha khoa và phục hồi nó, đặc biệt là khi liên quan đến một vị trí implant đơn lẻ. Implant có mặt cắt ngang tròn, trong khi răng tự nhiên thì không mà thay vào đó có mặt cắt ngang đỉnh thay đổi về hình dạng từ hình bầu dục sang hình tam giác sang hình chữ nhật tùy thuộc vào răng nào được thay thế. Khi yếu tố này được kết hợp với vùng giữa xương đỉnh và các tiếp xúc gần kề, một cấu hình nổi lên sẽ xuất hiện để điều chỉnh tính thẩm mỹ của phục hình. Cấu hình nổi lên cũng sẽ hướng dẫn và duy trì mô mềm, ngăn ngừa các vùng tích tụ thức ăn tiềm ẩn ở giữa. Bài viết này định nghĩa cấu hình nổi lên và xem xét tầm quan trọng của việc thiết lập nó và cách phát triển nó dựa trên răng được thay thế để đạt được phục hình có vẻ ngoài tự nhiên."} +{"idx": 1870802, "en": "Global change includes a substantial increase in the frequency and intensity of extreme high temperatures (EHTs), which influence insects at almost all levels. The number of studies showing the ecological importance of EHTs has risen in recent years, but the knowledge is rather dispersed in the contemporary literature. In this article, we review the biological and ecological effects of EHTs actually experienced in the field, i.e., when coupled to fluctuating thermal regimes. First, we characterize EHTs in the field. Then, we summarize the impacts of EHTs on insects at various levels and the processes allowing insects to buffer EHTs. Finally, we argue that the mechanisms leading to positive or negative impacts of EHTs on insects can only be resolved from integrative approaches considering natural thermal regimes. Thermal extremes, perhaps more than the gradual increase in mean temperature, drive insect responses to climate change, with crucial impacts on pest management and biodiversity conservation.", "gemini": "Biến đổi toàn cầu bao gồm sự gia tăng đáng kể về tần suất và cường độ của nhiệt độ cao cực độ (EHT), ảnh hưởng đến côn trùng ở hầu hết mọi cấp độ. Số lượng các nghiên cứu cho thấy tầm quan trọng về mặt sinh thái của EHT đã tăng lên trong những năm gần đây, nhưng kiến thức này lại khá phân tán trong các tài liệu đương đại. Trong bài viết này, chúng tôi xem xét các tác động sinh học và sinh thái của EHT thực sự xảy ra trong lĩnh vực này, tức là khi kết hợp với các chế độ nhiệt độ dao động. Đầu tiên, chúng tôi mô tả đặc điểm của EHT trong lĩnh vực này. Sau đó, chúng tôi tóm tắt các tác động của EHT đối với côn trùng ở nhiều cấp độ khác nhau và các quá trình cho phép côn trùng đệm EHT. Cuối cùng, chúng tôi lập luận rằng các cơ chế dẫn đến tác động tích cực hoặc tiêu cực của EHT đối với côn trùng chỉ có thể được giải quyết từ các phương pháp tiếp cận tích hợp xem xét các chế độ nhiệt tự nhiên. Các điều kiện nhiệt độ khắc nghiệt, có lẽ còn hơn cả sự gia tăng dần dần nhiệt độ trung bình, thúc đẩy phản ứng của côn trùng đối với biến đổi khí hậu, với những tác động quan trọng đến công tác quản lý dịch hại và bảo tồn đa dạng sinh học."} +{"idx": 1870803, "en": "Purpose This study examined the relationship between single word reading, connected text reading, and comprehension in persons with aphasia. Method Thirteen persons with aphasia read orally from the Arizona Battery for Reading and Spelling real-word and nonword lists and the Gray Oral Reading Tests-Fifth Edition. The comprehension questions following each paragraph of the Gray Oral Reading Tests-Fifth Edition were answered and scored. The Reading Comprehension Battery for Aphasia-Second Edition provided a measure of silent reading comprehension. Descriptive statistics and Spearman correlation were used to examine associations among reading measures. Results Persons with aphasia showed associations between single word reading and connected text reading accuracy; however, single word reading ability was not associated with oral or silent reading comprehension. Conclusions Although preliminary, the findings provide support for word-level reading abilities underlying connected text reading accuracy but suggest additional cognitive mechanisms are involved in text-level reading comprehension that are not explained by single word reading alone. The findings indicate clinicians should use caution when inferring comprehension abilities from single word reading performance as reading comprehension abilities are likely best assessed using text-level comprehension assessments.", "gemini": "Mục đích Nghiên cứu này kiểm tra mối quan hệ giữa việc đọc một từ, đọc văn bản kết nối và hiểu biết ở những người bị chứng mất ngôn ngữ. Phương pháp Mười ba người bị chứng mất ngôn ngữ đã đọc to từ Pin đọc và đánh vần Arizona danh sách từ thực và từ không phải từ và Bài kiểm tra đọc hiểu bằng miệng Gray-Phiên bản thứ năm. Các câu hỏi hiểu biết theo sau mỗi đoạn của Bài kiểm tra đọc hiểu bằng miệng Gray-Phiên bản thứ năm đã được trả lời và chấm điểm. Pin hiểu đọc cho chứng mất ngôn ngữ-Phiên bản thứ hai cung cấp một thước đo về khả năng hiểu đọc thầm. Thống kê mô tả và tương quan Spearman được sử dụng để kiểm tra các mối liên hệ giữa các biện pháp đọc. Kết quả Những người bị chứng mất ngôn ngữ cho thấy mối liên hệ giữa việc đọc một từ và độ chính xác khi đọc văn bản kết nối; tuy nhiên, khả năng đọc một từ không liên quan đến khả năng hiểu đọc hoặc đọc thầm. Kết luận Mặc dù chỉ mang tính sơ bộ, nhưng những phát hiện này cung cấp bằng chứng hỗ trợ cho các khả năng đọc ở cấp độ từ là cơ sở cho độ chính xác khi đọc văn bản kết nối nhưng cho thấy có thêm các cơ chế nhận thức liên quan đến khả năng hiểu đọc ở cấp độ văn bản mà không thể giải thích chỉ bằng cách đọc một từ. Những phát hiện này chỉ ra rằng các bác sĩ lâm sàng nên thận trọng khi suy luận khả năng hiểu từ hiệu suất đọc một từ vì khả năng hiểu đọc có khả năng được đánh giá tốt nhất bằng cách sử dụng các đánh giá hiểu ở cấp độ văn bản."} +{"idx": 1870804, "en": "BACKGROUND The role of fecal aerosols in the transmission of severe acute respiratory syndrome coronavirus 2 has been suspected. OBJECTIVE To investigate the temporal and spatial distributions of 3 infected families in a high-rise apartment building and examine the associated environmental variables to verify the role of fecal aerosols. DESIGN Epidemiologic survey and quantitative reverse transcriptase polymerase chain reaction analyses on throat swabs from the participants; 237 surface and air samples from 11 of the 83 flats in the building, public areas, and building drainage systems; and tracer gas released into bathrooms as a surrogate for virus-laden aerosols in the drainage system. SETTING A high-rise apartment building in Guangzhou, China. PARTICIPANTS 9 infected patients, 193 other residents of the building, and 24 members of the building's management staff. MEASUREMENTS Locations of infected flats and positive environmental samples, and spread of virus-laden aerosols. RESULTS 9 infected patients in 3 families were identified. The first family had a history of travel to the coronavirus disease 2019 (COVID-19) epicenter Wuhan, whereas the other 2 families had no travel history and a later onset of symptoms. No evidence was found for transmission via the elevator or elsewhere. The families lived in 3 vertically aligned flats connected by drainage pipes in the master bathrooms. Both the observed infections and the locations of positive environmental samples are consistent with the vertical spread of virus-laden aerosols via these stacks and vents. LIMITATION Inability to determine whether the water seals were dried out in the flats of the infected families. CONCLUSION On the basis of circumstantial evidence, fecal aerosol transmission may have caused the community outbreak of COVID-19 in this high-rise building. PRIMARY FUNDING SOURCE Key-Area Research and Development Program of Guangdong Province and the Research Grants Council of Hong Kong.", "gemini": "HOÀN CẢNH Vai trò của các hạt khí dung phân trong việc lây truyền virus corona gây hội chứng hô hấp cấp tính nặng 2 đã được nghi ngờ. MỤC TIÊU Nghiên cứu sự phân bố theo thời gian và không gian của 3 gia đình bị nhiễm bệnh trong một tòa chung cư cao tầng và kiểm tra các biến số môi trường liên quan để xác minh vai trò của các hạt khí dung phân. THIẾT KẾ Khảo sát dịch tễ học và phân tích phản ứng chuỗi phiên mã ngược định lượng trên dịch tỵ hầu của những người tham gia; 237 mẫu bề mặt và không khí từ 11 trong số 83 căn hộ trong tòa nhà, khu vực công cộng và hệ thống thoát nước của tòa nhà; và khí đánh dấu được thải vào phòng tắm như một chất thay thế cho các hạt khí dung chứa vi-rút trong hệ thống thoát nước. BỐI CẢNH Một tòa chung cư cao tầng ở Quảng Châu, Trung Quốc. NGƯỜI THAM GIA 9 bệnh nhân bị nhiễm bệnh, 193 cư dân khác của tòa nhà và 24 thành viên của đội ngũ quản lý tòa nhà. CÁC ĐO LƯỜNG Vị trí của các căn hộ bị nhiễm bệnh và các mẫu môi trường dương tính, và sự lây lan của các hạt khí dung chứa vi-rút. KẾT QUẢ 9 bệnh nhân bị nhiễm bệnh trong 3 gia đình đã được xác định. Gia đình đầu tiên có tiền sử đi du lịch đến ổ dịch bệnh do virus corona 2019 (COVID-19) là Vũ Hán, trong khi 2 gia đình còn lại không có tiền sử đi du lịch và các triệu chứng khởi phát muộn hơn. Không tìm thấy bằng chứng về sự lây truyền qua thang máy hoặc nơi khác. Các gia đình sống trong 3 căn hộ thẳng hàng được kết nối bằng đường ống thoát nước trong phòng tắm chính. Cả các trường hợp nhiễm trùng được quan sát thấy và vị trí của các mẫu môi trường dương tính đều phù hợp với sự lây lan theo chiều dọc của các hạt khí dung chứa vi-rút qua các ống khói và lỗ thông hơi này. HẠN CHẾ Không xác định được liệu các gioăng nước có bị khô trong các căn hộ của các gia đình bị nhiễm bệnh hay không. KẾT LUẬN Trên cơ sở bằng chứng tình huống, lây truyền qua khí dung phân có thể gây ra đợt bùng phát COVID-19 trong cộng đồng tại tòa nhà cao tầng này. NGUỒN TÀI TRỢ CHÍNH Chương trình Nghiên cứu và Phát triển Lĩnh vực trọng điểm của Tỉnh Quảng Đông và Hội đồng Tài trợ Nghiên cứu Hồng Kông."} +{"idx": 1870805, "en": "Purpose The purpose of this study was to report the variability of electrolarynx (EL) users' speech intelligibility in quiet and in multitalker babble. Method Ten EL users (five Servox® Digital, five TruTone™) who were at least 2 years postlaryngectomy provided recordings of five sentences from the 1965 Revised List of Phonetically Balanced Sentences. Recordings were judged by two groups of naïve listeners in quiet and in the presence of multitalker babble. Fifteen listeners orthographically transcribed a total of 750 sentences containing 3,750 key words in quiet, and another 15 listeners orthographically transcribed the same sentences mixed with multitalker babble. Results Significant differences in speech intelligibility were observed between listening conditions; 17.9% more key words were correctly identified in quiet compared to multitalker babble. Significant differences in fundamental frequency (F0) standard deviation and range but not speech intelligibility were observed between EL device types. A positive correlation of moderate significance was observed between F0 standard deviation and intelligibility for TruTone users in multitalker babble. Conclusions Findings suggest that listeners are able to identify a significantly higher percentage of EL users' speech in quiet compared to multitalker babble, but a large variability in EL users' speech intelligibility exists. Continued investigation involving a larger number of EL users is necessary to confirm this study's findings. Future research should explore the relationships among F0 measures, speaker characteristics (e.g., rate of speech, articulatory precision), and speech intelligibility, in addition to improving alaryngeal rehabilitation training protocols for EL users.", "gemini": "Mục đích Mục đích của nghiên cứu này là báo cáo về khả năng hiểu lời nói thay đổi của những người dùng thanh quản điện tử (EL) trong môi trường yên tĩnh và trong tiếng ồn ào của nhiều người nói chuyện. Phương pháp Mười người dùng EL (năm người dùng Servox® Digital, năm người dùng TruTone™) đã cắt thanh quản ít nhất 2 năm đã cung cấp bản ghi âm năm câu từ Danh sách Câu cân bằng ngữ âm được Sửa đổi năm 1965. Bản ghi âm được hai nhóm người nghe ngây thơ đánh giá trong môi trường yên tĩnh và trong điều kiện có tiếng ồn ào của nhiều người nói chuyện. Mười lăm người nghe đã phiên âm chính tả tổng cộng 750 câu có chứa 3.750 từ khóa trong môi trường yên tĩnh và 15 người nghe khác đã phiên âm chính tả các câu tương tự khi trộn với tiếng ồn ào của nhiều người nói chuyện. Kết quả Có sự khác biệt đáng kể về khả năng hiểu lời nói giữa các điều kiện nghe; 17,9% từ khóa được xác định chính xác trong môi trường yên tĩnh so với tiếng ồn ào của nhiều người nói chuyện. Có sự khác biệt đáng kể về độ lệch chuẩn tần số cơ bản (F0) và phạm vi nhưng không phải khả năng hiểu lời nói giữa các loại thiết bị EL. Có mối tương quan dương có ý nghĩa vừa phải giữa độ lệch chuẩn F0 và khả năng hiểu của người dùng TruTone trong tiếng ồn ào của nhiều người nói chuyện. Kết luận Các phát hiện cho thấy người nghe có thể xác định được tỷ lệ phần trăm lời nói của người dùng EL cao hơn đáng kể trong môi trường yên tĩnh so với tiếng ồn ào của nhiều người nói chuyện, nhưng khả năng hiểu lời nói của người dùng EL lại rất khác nhau. Cần tiếp tục điều tra liên quan đến nhiều người dùng EL hơn để xác nhận những phát hiện của nghiên cứu này. Nghiên cứu trong tương lai nên khám phá mối quan hệ giữa các biện pháp F0, đặc điểm của người nói (ví dụ: tốc độ nói, độ chính xác về mặt phát âm) và khả năng hiểu lời nói, ngoài việc cải thiện các giao thức đào tạo phục hồi không thanh quản cho người dùng EL."} +{"idx": 1870806, "en": "BACKGROUND Childhood-onset cardiomyopathy is a heterogeneous group of conditions the cause of which is largely unknown. The influence of consanguinity on the genetics of cardiomyopathy has not been addressed at a large scale. METHODS To unravel the genetic cause of childhood-onset cardiomyopathy in a consanguineous population, a categorized approach was adopted. Cases with childhood-onset cardiomyopathy were consecutively recruited. Based on the likelihood of founder mutation and on the clinical diagnosis, genetic test was categorized to either (1) targeted genetic test with targeted mutation test, single-gene test, or multigene panel for Noonan syndrome, or (2) untargeted genetic test with whole-exome sequencing or whole-genome sequencing. Several bioinformatics tools were used to filter the variants. RESULTS Two-hundred five unrelated probands with various forms of cardiomyopathy were evaluated. The median age of presentation was 10 months. In 30.2% (n=62), targeted genetic test had a yield of 82.7% compared with 33.6% for whole-exome sequencing/whole-genome sequencing (n=143) giving an overall yield of 53.7%. Strikingly, 96.4% of the variants were homozygous, 9% of which were found in 4 dominant genes. Homozygous variants were also detected in 7 novel candidates (ACACB, AASDH, CASZ1, FLII, RHBDF1, RPL3L, ULK1). CONCLUSIONS Our work demonstrates the impact of consanguinity on the genetics of childhood-onset cardiomyopathy, the value of adopting a categorized population-sensitive genetic approach, and the opportunity of uncovering novel genes. Our data suggest that if a founder mutation is not suspected, adopting whole-exome sequencing/whole-genome sequencing as a first-line test should be considered.", "gemini": "HOÀN CẢNH Bệnh cơ tim khởi phát ở trẻ em là một nhóm các tình trạng không đồng nhất mà nguyên nhân phần lớn vẫn chưa được biết đến. Ảnh hưởng của quan hệ cận huyết đến di truyền của bệnh cơ tim vẫn chưa được giải quyết ở quy mô lớn. PHƯƠNG PHÁP Để làm sáng tỏ nguyên nhân di truyền của bệnh cơ tim khởi phát ở trẻ em trong quần thể cận huyết, một phương pháp tiếp cận được phân loại đã được áp dụng. Các trường hợp mắc bệnh cơ tim khởi phát ở trẻ em được tuyển dụng liên tiếp. Dựa trên khả năng đột biến của người sáng lập và chẩn đoán lâm sàng, xét nghiệm di truyền được phân loại thành (1) xét nghiệm di truyền mục tiêu với xét nghiệm đột biến mục tiêu, xét nghiệm gen đơn hoặc bảng gen đa gen cho hội chứng Noonan, hoặc (2) xét nghiệm di truyền không mục tiêu với giải trình toàn bộ exome hoặc giải trình toàn bộ hệ gen. Một số công cụ tin sinh học đã được sử dụng để lọc các biến thể. KẾT QUẢ Hai trăm năm bệnh nhân không có quan hệ họ hàng mắc các dạng bệnh cơ tim khác nhau đã được đánh giá. Độ tuổi trung bình khi biểu hiện là 10 tháng. Ở 30,2% (n = 62), xét nghiệm di truyền mục tiêu có hiệu suất là 82,7% so với 33,6% đối với giải trình toàn bộ exome/giải trình toàn bộ hệ gen (n = 143) cho hiệu suất chung là 53,7%. Đáng ngạc nhiên là 96,4% các biến thể là đồng hợp tử, 9% trong số đó được tìm thấy ở 4 gen trội. Các biến thể đồng hợp tử cũng được phát hiện ở 7 ứng viên mới (ACACB, AASDH, CASZ1, FLII, RHBDF1, RPL3L, ULK1). KẾT LUẬN Công trình của chúng tôi chứng minh tác động của quan hệ cận huyết đến di truyền của bệnh cơ tim khởi phát ở trẻ em, giá trị của việc áp dụng phương pháp tiếp cận di truyền nhạy cảm với quần thể được phân loại và cơ hội phát hiện các gen mới. Dữ liệu của chúng tôi cho thấy rằng nếu không nghi ngờ đột biến của người sáng lập, thì nên cân nhắc việc áp dụng giải trình toàn bộ exome/giải trình toàn bộ hệ gen làm xét nghiệm tuyến đầu."} +{"idx": 1870807, "en": "PURPOSE To investigate the validity and within-day reliability of the Glittre activities of daily living test (Glittre ADL-test) in patients on hemodialysis. METHODS Ninety-one patients on hemodialysis were evaluated using Glittre ADL-test, functional tests, health-related quality of life, human activity profile, and social participation. Convergent validity was analyzed by the correlation analysis. The Glittre ADL-test results were compared among different levels of the functional tests, human activity profile, and participation (known-groups validity). Within-day reliability was examined by Intra-class Correlation Coefficient (ICC). RESULTS There was a significant moderate to strong correlation between Glittre ADL-test and functional tests (r = 0.62 to 0.66 and -0.70 to -0.44). A significant weak correlation was found between Glittre ADL-test, health-related quality of life (r = 0.28 to 0.32), and human activity profile (r=-0.33). There was no significant correlation with participation. Except for participation level, the Glittre ADL-test results were able to identify individuals with low performance in all evaluated tests and were able to differentiate among inactive and active individuals by human activity profile (p < 0.05). Within-day reliability was evaluated in 42 patients. The ICC was 0.97. CONCLUSION The Glittre ADL-test is a reliable test associated with functional parameters and physical aspects related to health-related quality of life, with potential value in the functional evaluation of patients on hemodialysis.Implications for rehabilitationFunctional impairment is a common clinical finding and is an important predictor of poor prognosis in patients on hemodialysis.The Glittre ADL-test has excellent within-day reliability and is a valid instrument for functional assessment in patients on hemodialysis.Activity limitations of patients on hemodialysis can be assessed by Glittre ADL-test.", "gemini": "MỤC ĐÍCH Nghiên cứu độ tin cậy và tính tin cậy trong ngày của bài kiểm tra hoạt động sinh hoạt hằng ngày Glittre (bài kiểm tra ADL-Glittre) ở những bệnh nhân đang chạy thận nhân tạo. PHƯƠNG PHÁP Chín mươi mốt bệnh nhân đang chạy thận nhân tạo được đánh giá bằng bài kiểm tra ADL-Glittre, các xét nghiệm chức năng, chất lượng cuộc sống liên quan đến sức khỏe, hồ sơ hoạt động của con người và sự tham gia xã hội. Độ tin cậy hội tụ được phân tích bằng phân tích tương quan. Kết quả bài kiểm tra ADL-Glittre được so sánh giữa các mức khác nhau của các xét nghiệm chức năng, hồ sơ hoạt động của con người và sự tham gia (độ tin cậy của các nhóm đã biết). Độ tin cậy trong ngày được kiểm tra bằng Hệ số tương quan nội nhóm (ICC). KẾT QUẢ Có một mối tương quan vừa phải đến mạnh có ý nghĩa giữa bài kiểm tra ADL-Glittre và các xét nghiệm chức năng (r = 0,62 đến 0,66 và -0,70 đến -0,44). Một mối tương quan yếu có ý nghĩa được tìm thấy giữa bài kiểm tra ADL-Glittre, chất lượng cuộc sống liên quan đến sức khỏe (r = 0,28 đến 0,32) và hồ sơ hoạt động của con người (r = -0,33). Không có mối tương quan có ý nghĩa nào với sự tham gia. Ngoại trừ mức độ tham gia, kết quả bài kiểm tra ADL-Glittre có thể xác định những cá nhân có kết quả thấp trong tất cả các xét nghiệm được đánh giá và có thể phân biệt giữa những cá nhân không hoạt động và hoạt động theo hồ sơ hoạt động của con người (p < 0,05). Độ tin cậy trong ngày được đánh giá ở 42 bệnh nhân. ICC là 0,97. KẾT LUẬN Bài kiểm tra ADL-Glittre là một bài kiểm tra đáng tin cậy liên quan đến các thông số chức năng và các khía cạnh thể chất liên quan đến chất lượng cuộc sống liên quan đến sức khỏe, có giá trị tiềm năng trong việc đánh giá chức năng của bệnh nhân đang chạy thận nhân tạo. Ý nghĩa đối với phục hồi chức năng Suy giảm chức năng là một phát hiện lâm sàng phổ biến và là một yếu tố dự báo quan trọng về tiên lượng kém ở những bệnh nhân đang chạy thận nhân tạo. Bài kiểm tra ADL-Glittre có độ tin cậy trong ngày tuyệt vời và là một công cụ hợp lệ để đánh giá chức năng ở những bệnh nhân đang chạy thận nhân tạo. Hạn chế hoạt động của bệnh nhân đang chạy thận nhân tạo có thể được đánh giá bằng bài kiểm tra ADL-Glittre."} +{"idx": 1870808, "en": "The emotional texture of colorectal cancer treatments in a British oncology clinic provides insight into the ways that patients, their relatives, and health professionals deal with the suffering that cancer poses. In cancer care, verbalizing emotional reactions is understood as a healthy way of dealing with troublesome emotions. Yet, a type of silence, here understood as \"not getting upset in front of each other,\" helps participants to preserve relationships. This idea contributes to our understanding of silence as a relational form of moral work that seeks to preserve the well-being of others.", "gemini": "Cảm xúc của các phương pháp điều trị ung thư trực tràng tại một phòng khám ung thư ở Anh cung cấp cái nhìn sâu sắc về cách mà bệnh nhân, người thân của họ và các chuyên gia y tế đối phó với nỗi đau mà căn bệnh ung thư gây ra. Trong việc chăm sóc bệnh nhân ung thư, việc thể hiện các phản ứng cảm xúc được hiểu là một cách lành mạnh để đối phó với những cảm xúc phiền muộn. Tuy nhiên, một kiểu im lặng, ở đây được hiểu là \"không làm phiền nhau\", giúp những người tham gia duy trì các mối quan hệ. Ý tưởng này góp phần vào sự hiểu biết của chúng ta về sự im lặng như một hình thức công việc đạo đức có mối quan hệ nhằm duy trì sự khỏe mạnh của người khác."} +{"idx": 1870809, "en": "We report on clinical outcomes in patients with oligometastatic uterine cancer treated with stereotactic body radiation therapy (SBRT). Twenty-seven patients with 61 lesions were treated with SBRT. Median follow-up was 16.9 months. Local control was achieved in 49/61 (80.3%) lesions. One-year local-progression-free survival and overall survival were 75.9% and 65.4%. Lesions with favorable response were smaller than lesions with unfavorable response (p = .007). Liver lesions were less likely to achieve favorable response (p = .0128). There were no grade 3 or 4 events. Treatment with SBRT can provide excellent local control in oligometastatic uterine cancer with minimal toxicity.", "gemini": "Chúng tôi báo cáo về kết quả lâm sàng ở những bệnh nhân ung thư tử cung di căn ít được điều trị bằng xạ trị lập thể (SBRT). Hai mươi bảy bệnh nhân với 61 tổn thương đã được điều trị bằng SBRT. Thời gian theo dõi trung bình là 16,9 tháng. Kiểm soát tại chỗ đạt được ở 49/61 (80,3%) tổn thương. Tỷ lệ sống không tiến triển tại chỗ một năm và tỷ lệ sống tổng thể là 75,9% và 65,4%. Các tổn thương có đáp ứng thuận lợi nhỏ hơn các tổn thương có đáp ứng không thuận lợi (p = 0,007). Các tổn thương ở gan ít có khả năng đạt được đáp ứng thuận lợi (p = 0,0128). Không có sự kiện nào ở mức độ 3 hoặc 4. Điều trị bằng SBRT có thể kiểm soát tại chỗ tuyệt vời ở ung thư tử cung di căn ít với độc tính tối thiểu."} +{"idx": 1870810, "en": "The survival rates of youth and young adults (YYAs) diagnosed with long-term conditions have improved considerably as 90% now enter adulthood; health care transition planning (HCTP) has emerged as a nursing practice priority. The aim of this national online survey was to investigate the extent to which nurses, recruited from two major United States pediatric nursing organizations are involved with HCTP including assessing YYA self-management abilities (SMA). Findings of a 9-item assessment of self-management abilities subscale of the nurses' role in HTCP tool are reported. The nurse respondents (n = 1269), identified the most frequently assessed SMA was the YYAs' ability to understand and speak about their condition and its treatment (M = 2.3, SD = .89). The least frequently assessed was the YYAs' ability to identify community advocates to help them become more independent (M =1.5, SD = .90). Regression analysis identified significant predictors of the frequency nurses assess YYA for SMA included nurses' level of knowledge, perceived level of importance, HCTP and skills identified in job description, and caring for YYA. Findings indicate HCTP care advancements will necessitate HCTP training and development of nurse-led service efforts to facilitate optimal outcomes for YYA.", "gemini": "Tỷ lệ sống sót của thanh thiếu niên và người trẻ tuổi (YYA) được chẩn đoán mắc các bệnh lâu dài đã được cải thiện đáng kể khi 90% hiện đã bước vào tuổi trưởng thành; lập kế hoạch chuyển đổi chăm sóc sức khỏe (HCTP) đã trở thành ưu tiên thực hành điều dưỡng. Mục đích của cuộc khảo sát trực tuyến toàn quốc này là điều tra mức độ mà các y tá được tuyển dụng từ hai tổ chức điều dưỡng nhi khoa lớn của Hoa Kỳ tham gia vào HCTP bao gồm đánh giá khả năng tự quản lý (SMA) của YYA. Các phát hiện của đánh giá 9 mục về khả năng tự quản lý của thang đánh giá vai trò của y tá trong công cụ HTCP được báo cáo. Những người trả lời là y tá (n = 1269) xác định SMA được đánh giá thường xuyên nhất là khả năng hiểu và nói về tình trạng bệnh và cách điều trị của YYA (M = 2,3, SD = 0,89). Ít được đánh giá thường xuyên nhất là khả năng xác định những người ủng hộ cộng đồng để giúp họ trở nên độc lập hơn (M = 1,5, SD = 0,90). Phân tích hồi quy xác định các yếu tố dự báo đáng kể về tần suất y tá đánh giá YYA đối với SMA bao gồm trình độ kiến thức của y tá, mức độ quan trọng được nhận thức, HCTP và các kỹ năng được xác định trong mô tả công việc và chăm sóc YYA. Những phát hiện chỉ ra rằng những tiến bộ trong chăm sóc HCTP sẽ đòi hỏi đào tạo HCTP và phát triển các nỗ lực dịch vụ do y tá lãnh đạo để tạo điều kiện đạt được kết quả tối ưu cho YYA."} +{"idx": 1870811, "en": "This integrative review explored nursing assessment of youth who self-harm. Eleven papers published between 2000 to 2020 met inclusion criteria. Three themes emerged: \"Doctors and nurses' perceptions of assessment of self-harm\", \"Attitudes and experiences of assessment of self-harm\" and \"Assessments and outcomes\". The ways youth communicate is an important consideration when assessing and planning for care. The metaphor of Narcissus is used to understand youth beliefs, self-image and how they communicate these thoughts. This paper suggests an alternative reading of what young people, who presents with self-harm, are trying to communicate to others and how this is integrated into assessment.", "gemini": "Đánh giá tổng hợp này đã khám phá đánh giá điều dưỡng của những thanh thiếu niên tự gây hại. Mười một bài báo được công bố từ năm 2000 đến năm 2020 đáp ứng các tiêu chí đưa vào. Ba chủ đề nổi lên: \"Nhận thức của bác sĩ và y tá về đánh giá tự gây hại\", \"Thái độ và kinh nghiệm đánh giá tự gây hại\" và \"Đánh giá và kết quả\". Cách giao tiếp của thanh thiếu niên là một cân nhắc quan trọng khi đánh giá và lập kế hoạch chăm sóc. Ẩn dụ về Narcissus được sử dụng để hiểu niềm tin của thanh thiếu niên, hình ảnh bản thân và cách họ truyền đạt những suy nghĩ này. Bài báo này gợi ý một cách đọc khác về những gì mà những người trẻ tuổi, những người tự gây hại, đang cố gắng truyền đạt cho người khác và cách điều này được tích hợp vào đánh giá."} +{"idx": 1870812, "en": "There is a growing interest in identifying the psychological variables that promote and sustain empathy in medical students during their studies. Dispositional mindfulness has been shown to be empirically associated with socio-demographic characteristics and empathy among the general population. This research aimed to assess dispositional mindfulness in a sample of undergraduate medical students and to investigate its association with gender, age, and empathy. Hypotheses: It is hypothesized that male medical students would show, on average, higher dispositional mindfulness than their female counterparts, and that older students would exhibit higher dispositional mindfulness than younger ones. Dispositional mindfulness was also expected to be positively associated with the ability to feel compassion for others and to adopt their perspective, and negatively associated with the personal distress in tense interpersonal settings. Method: An exploratory cross-sectional study was conducted. Data were gathered from a large sample (N = 933) of Italian non-meditating second- and fifth-year medical students. Dispositional mindfulness and empathy were assessed using the Five-Facet Mindfulness Questionnaire and the Interpersonal Reactivity Index, respectively. Gender and age differences in dispositional mindfulness scores were calculated by analyses of variance, whereas hierarchical multiple regression models were used to assess the association between dispositional mindfulness and empathy scores. RESULTS Female medical students were more able to Act with Awareness than males, whereas males had higher levels than females of Describing and Nonreactivity to their feelings. When compared to their older counterparts, younger students scored higher on Observing and lower on Nonreactivity facets. Dispositional mindfulness facets correlated differently with both emotional and cognitive empathy dimensions, beyond the effects of gender and age. Medical students who displayed higher dispositional mindfulness appeared to be less emotionally distressed in tense interpersonal settings and more able to take others' cognitive perspective. Conclusions: The findings support the notion that dispositional mindfulness is related to empathy and may have implications for the design of mindfulness-based training for use in the medical educational setting. Tailored interventions that cultivate specific dispositional mindfulness facets may be implemented along the medical curriculum to prevent the emotional distress in tense interpersonal settings and to sustain the cognitive capability to take others' viewpoints among medical students.", "gemini": "Có một sự quan tâm ngày càng tăng trong việc xác định các biến số tâm lý thúc đẩy và duy trì sự đồng cảm ở sinh viên y trong quá trình học tập của họ. Sự chánh niệm về tính cách đã được chứng minh là có liên quan thực nghiệm với các đặc điểm nhân khẩu học xã hội và sự đồng cảm trong dân số nói chung. Nghiên cứu này nhằm đánh giá sự chánh niệm về tính cách trong một mẫu sinh viên y đại học và điều tra mối liên hệ của nó với giới tính, tuổi tác và sự đồng cảm. Giả thuyết: Người ta giả định rằng sinh viên y nam sẽ thể hiện sự chánh niệm về tính cách cao hơn so với sinh viên nữ và sinh viên lớn tuổi sẽ thể hiện sự chánh niệm về tính cách cao hơn so với sinh viên nhỏ tuổi. Sự chánh niệm về tính cách cũng được kỳ vọng là có liên quan tích cực với khả năng cảm thông với người khác và chấp nhận quan điểm của họ, và có liên quan tiêu cực với sự đau khổ cá nhân trong các hoàn cảnh căng thẳng giữa các cá nhân. Phương pháp: Một nghiên cứu cắt ngang thăm dò đã được tiến hành. Dữ liệu được thu thập từ một mẫu lớn (N = 933) sinh viên y năm thứ hai và thứ năm không thiền định của Ý. Sự chánh niệm về tính cách và sự đồng cảm được đánh giá lần lượt bằng Bản câu hỏi về chánh niệm năm khía cạnh và Chỉ số phản ứng giữa các cá nhân. Sự khác biệt về giới tính và tuổi tác trong số điểm chánh niệm về tính cách được tính toán bằng phân tích phương sai, trong khi các mô hình hồi quy bội bậc phân cấp được sử dụng để đánh giá mối liên hệ giữa sự chánh niệm về tính cách và điểm đồng cảm. KẾT QUẢ Sinh viên y nữ có khả năng Hành động với nhận thức cao hơn nam giới, trong khi nam giới có mức độ Mô tả và Không phản ứng cao hơn nữ giới đối với cảm xúc của họ. Khi so sánh với những người lớn tuổi hơn, những sinh viên trẻ hơn đạt điểm cao hơn ở các khía cạnh Quan sát và thấp hơn ở các khía cạnh Không phản ứng. Các khía cạnh chánh niệm về tính cách có mối tương quan khác nhau với cả các chiều đồng cảm về mặt cảm xúc và nhận thức, ngoài các ảnh hưởng của giới tính và tuổi tác. Những sinh viên y có sự chánh niệm về tính cách cao hơn dường như ít đau khổ về mặt cảm xúc hơn trong các hoàn cảnh căng thẳng giữa các cá nhân và có khả năng tiếp nhận quan điểm nhận thức của người khác hơn. Kết luận: Những phát hiện này hỗ trợ quan điểm cho rằng sự chánh niệm về tính cách có liên quan đến sự đồng cảm và có thể có ý nghĩa đối với việc thiết kế đào tạo dựa trên sự chánh niệm để sử dụng trong môi trường giáo dục y khoa. Các biện pháp can thiệp được điều chỉnh để bồi dưỡng các khía cạnh chánh niệm về tính cách cụ thể có thể được thực hiện trong suốt chương trình giảng dạy y khoa để ngăn ngừa sự đau khổ về mặt cảm xúc trong các hoàn cảnh căng thẳng giữa các cá nhân và duy trì khả năng nhận thức để tiếp nhận quan điểm của người khác trong số các sinh viên y."} +{"idx": 1870813, "en": "A clean colon is required for a colonoscopy to be effective. Therefore, patients undergo a bowel preparation in advance of the procedure to clear the colon. Typically, this involves drinking 2 litres of polyethylene glycol or macrogol (PEG) solution, but dislike of the taste or having to drink such a large volume causes some patients not to adhere to this regimen. To address this, a PEG solution has been developed that requires patients to drink only 1 litre of bowel preparation in two flavours. The hope is this will increase patient adherence.", "gemini": "Để nội soi đại tràng có hiệu quả thì phải làm sạch đại tràng. Do đó, trước khi tiến hành thủ thuật, bệnh nhân phải chuẩn bị nhuận tràng để làm sạch đại tràng. Thông thường, việc này bao gồm việc uống 2 lít dung dịch polyethylene glycol hoặc macrogol (PEG), nhưng một số bệnh nhân không tuân thủ chế độ này vì không thích mùi vị hoặc phải uống một lượng lớn như vậy. Để giải quyết vấn đề này, người ta đã phát triển một dung dịch PEG chỉ yêu cầu bệnh nhân uống 1 lít thuốc nhuận tràng có hai hương vị. Hy vọng rằng điều này sẽ làm tăng sự tuân thủ của bệnh nhân."} +{"idx": 1870814, "en": "Due to concerns surrounding travel during the COVID-19 pandemic, the 2020-2021 otolaryngology residency application cycle will be conducted virtually for the first time. Residency programs should consider the logistics of video interviews, drawing on experiences of other programs that have successfully performed virtual interviews in the past. The lack of in-person interviews will create challenges in assessing applicants, and we recommend that programs develop structured and targeted questions and even consider having candidates answer standardized questions prior to the virtual interview day. From an applicant perspective, gauging the intangibles of individual residency programs, such as resident camaraderie, program culture, and program location, will be difficult. To address this, programs should consider hosting informal virtual gatherings, create videos that highlight the resident experience, and ensure that program websites are up-to-date. Ultimately, adaptability, resilience, and innovation will allow residency programs to achieve a successful 2021 otolaryngology match.", "gemini": "Do những lo ngại xung quanh việc đi lại trong đại dịch COVID-19, chu kỳ nộp đơn xin cư trú về tai mũi họng năm 2020-2021 sẽ lần đầu tiên được tiến hành trực tuyến. Các chương trình cư trú nên xem xét hậu cần của các cuộc phỏng vấn qua video, dựa trên kinh nghiệm của các chương trình khác đã thực hiện thành công các cuộc phỏng vấn trực tuyến trong quá khứ. Việc không có các cuộc phỏng vấn trực tiếp sẽ tạo ra những thách thức trong việc đánh giá ứng viên và chúng tôi khuyên các chương trình nên phát triển các câu hỏi có cấu trúc và mục tiêu, thậm chí xem xét yêu cầu ứng viên trả lời các câu hỏi chuẩn hóa trước ngày phỏng vấn trực tuyến. Theo quan điểm của ứng viên, việc đánh giá các yếu tố vô hình của từng chương trình cư trú, chẳng hạn như tình đồng chí của các bác sĩ nội trú, văn hóa chương trình và địa điểm chương trình, sẽ rất khó khăn. Để giải quyết vấn đề này, các chương trình nên xem xét việc tổ chức các buổi họp mặt trực tuyến không chính thức, tạo các video nêu bật trải nghiệm của bác sĩ nội trú và đảm bảo rằng các trang web của chương trình được cập nhật. Cuối cùng, khả năng thích ứng, khả năng phục hồi và sự đổi mới sẽ giúp các chương trình cư trú đạt được sự kết hợp thành công về tai mũi họng năm 2021."} +{"idx": 1870815, "en": "Thromboelastography (TEG) is regularly used for monitoring abnormalities of the coagulation system in patients with sepsis. However, it is unclear whether TEG parameters are associated with sepsis-induced coagulopathy (SIC). Thus, we aimed to assess the diagnostic value of TEG for SIC. The medical records of patients who underwent TEG from January 2016 to December 2016 were analyzed retrospectively. The patients were divided into sepsis group and non-sepsis group. Baseline patient characteristics and coagulation function indexes were compared. Receiver-operating characteristic curve analysis was used to determine predictors of SIC. A total of 167 patients were included, of whom 84 had sepsis. The clot formation speed (K) was significantly higher(P < 0.001), and the maximum amplitude (MA) and angle were significantly lower (both P < 0.001) in the sepsis group than that in non-sepsis group. Patients with SIC had higher Sepsis-related Organ Failure Assessment scores than those patients without SIC (P < 0.001). The area under the curve of K for diagnosing SIC was 0.910. The area under the curve of angle and MA for excluding SIC was 0.895 and 0.882, respectively. Thus, TEG parameters have good diagnostic value for SIC.", "gemini": "Thromboelastography (TEG) thường được sử dụng để theo dõi các bất thường của hệ thống đông máu ở những bệnh nhân bị nhiễm trùng huyết. Tuy nhiên, không rõ các thông số TEG có liên quan đến tình trạng đông máu do nhiễm trùng huyết (SIC) hay không. Do đó, chúng tôi muốn đánh giá giá trị chẩn đoán của TEG đối với SIC. Hồ sơ bệnh án của những bệnh nhân đã làm TEG từ tháng 1 năm 2016 đến tháng 12 năm 2016 đã được phân tích hồi cứu. Các bệnh nhân được chia thành nhóm nhiễm trùng huyết và nhóm không nhiễm trùng huyết. Đặc điểm cơ bản của bệnh nhân và các chỉ số chức năng đông máu được so sánh. Phân tích đường cong đặc tính hoạt động của máy thu được sử dụng để xác định các yếu tố dự báo SIC. Tổng cộng có 167 bệnh nhân được đưa vào nghiên cứu, trong đó 84 bệnh nhân bị nhiễm trùng huyết. Tốc độ hình thành cục máu đông (K) cao hơn đáng kể (P < 0,001) và biên độ tối đa (MA) và góc thấp hơn đáng kể (cả hai đều P < 0,001) ở nhóm nhiễm trùng huyết so với nhóm không nhiễm trùng huyết. Bệnh nhân bị SIC có điểm Đánh giá suy đa cơ quan liên quan đến nhiễm trùng huyết cao hơn so với những bệnh nhân không bị SIC (P < 0,001). Diện tích dưới đường cong của K để chẩn đoán SIC là 0,910. Diện tích dưới đường cong của góc và MA để loại trừ SIC lần lượt là 0,895 và 0,882. Do đó, các thông số TEG có giá trị chẩn đoán tốt đối với SIC."} +{"idx": 1870816, "en": "BACKGROUND Strokes are a leading cause of disability in the United States. Approximately 25-40% of individuals who experience a stroke will have aphasia. Individuals with aphasia experience many life changes and some have found that writing activities, such as publishing text entries on a personal website, or weblog, to be therapeutic. PURPOSE This study aimed to gain a greater understanding of the therapeutic use of blogs for stroke survivors with aphasia. DESIGN Inductive qualitative content analysis. METHODS Potential, publicly available weblogs written by people with aphasia were identified using a pre-determined search method and five met inclusion criteria. Data were collected from websites and collated into 453 pages of narratives. An inductive qualitative content analysis process was used to extract codes that were reduced to sub-categories and generic categories. RESULTS A main category of therapeutic healing and three generic categories of (1) living with aphasia, (2) accepting a new identity, and (3) creating a community were identified. CONCLUSION Weblog writing was therapeutic for stroke survivors with aphasia. Authors reported benefits across cognitive, psychological, emotional, and social domains. Health professionals should consider recommending weblog writing, journaling, or other methods of writing for stroke survivors with aphasia.Implications for rehabilitationCreating illness weblog posts can be therapeutically beneficial for stroke survivors with aphasia as a tool that addresses psychological, emotional, and social aspects in their recovery.Blogging appeared to offer a medium for stroke survivors with aphasia to practice writing, to express what living with aphasia is like, to explore changes in their post-stroke identities, and to create a community with others.Health professionals should consider recommending weblog writing, journaling, or other methods of writing for stroke survivors with aphasia.", "gemini": "HOÀN CẢNH Đột quỵ là nguyên nhân hàng đầu gây ra tình trạng tàn tật tại Hoa Kỳ. Khoảng 25-40% những người bị đột quỵ sẽ mắc chứng mất ngôn ngữ. Những người bị mất ngôn ngữ phải trải qua nhiều thay đổi trong cuộc sống và một số người thấy rằng các hoạt động viết lách, chẳng hạn như đăng các mục văn bản trên trang web cá nhân hoặc weblog, có tác dụng chữa bệnh. MỤC ĐÍCH Nghiên cứu này nhằm mục đích hiểu rõ hơn về việc sử dụng blog như một liệu pháp điều trị cho những người sống sót sau đột quỵ bị mất ngôn ngữ. THIẾT KẾ Phân tích nội dung định tính quy nạp. PHƯƠNG PHÁP Các weblog có khả năng, có sẵn công khai do những người bị mất ngôn ngữ viết đã được xác định bằng cách sử dụng phương pháp tìm kiếm được xác định trước và năm tiêu chí bao gồm. Dữ liệu được thu thập từ các trang web và được tập hợp thành 453 trang tường thuật. Một quy trình phân tích nội dung định tính quy nạp đã được sử dụng để trích xuất các mã đã được rút gọn thành các tiểu loại và các loại chung. KẾT QUẢ Một loại chính về chữa bệnh bằng liệu pháp và ba loại chung về (1) sống chung với chứng mất ngôn ngữ, (2) chấp nhận một danh tính mới và (3) tạo ra một cộng đồng đã được xác định. KẾT LUẬN Viết weblog có tác dụng chữa bệnh cho những người sống sót sau đột quỵ bị mất ngôn ngữ. Các tác giả đã báo cáo lợi ích trên các lĩnh vực nhận thức, tâm lý, cảm xúc và xã hội. Các chuyên gia y tế nên cân nhắc việc đề xuất viết weblog, viết nhật ký hoặc các phương pháp viết khác cho những người sống sót sau đột quỵ bị mất ngôn ngữ.Ý nghĩa đối với phục hồi chức năngViệc tạo bài đăng trên weblog về bệnh tật có thể có lợi ích về mặt trị liệu cho những người sống sót sau đột quỵ bị mất ngôn ngữ như một công cụ giải quyết các khía cạnh tâm lý, cảm xúc và xã hội trong quá trình phục hồi của họ.Viết blog dường như cung cấp một phương tiện cho những người sống sót sau đột quỵ bị mất ngôn ngữ để thực hành viết, để bày tỏ cảm nhận về việc sống chung với chứng mất ngôn ngữ, để khám phá những thay đổi trong danh tính của họ sau đột quỵ và để tạo ra một cộng đồng với những người khác.Các chuyên gia y tế nên cân nhắc việc đề xuất viết weblog, viết nhật ký hoặc các phương pháp viết khác cho những người sống sót sau đột quỵ bị mất ngôn ngữ."} +{"idx": 1870817, "en": "INTRODUCTION Bronchoscopic lung volume reduction (BLVR) is a novel approach for treatment of emphysema. Several techniques are available to accomplish BLVR including Bronchoscopic Vapor Thermal Ablation (BVTA). This technique is easy to perform and considered safe due to its gradual effect. We discuss BTVA in detail in this editorial. AREAS COVERED We discuss our experience with BTVA in detail including patient selection, equipment, procedure, post-procedural care and complications. We also review the literature to determine the pros and cons for its use. Other modalities such as endobronchial valves, coils and lung sealants are also briefly discussed. EXPERT OPINION Vapor ablation is a novel and safe approach in inducing lung volume reduction in emphysema patients. The effects are gradual, and therefore potentially making it safer than other minimally invasive modalities. Pneumonitis and infection are common side effects. Just as in other BLVR techniques, a case by case evaluation is needed to determine the right candidate for BTVA. Further larger studies are needed before BTVA becomes standard of care in treatment of patients with emphysema.", "gemini": "GIỚI THIỆU Giảm thể tích phổi bằng nội soi phế quản (BLVR) là một phương pháp tiếp cận mới để điều trị bệnh phổi khí phế thũng. Có một số kỹ thuật có thể thực hiện được BLVR bao gồm Phẫu thuật cắt bỏ hơi nhiệt bằng nội soi phế quản (BVTA). Kỹ thuật này dễ thực hiện và được coi là an toàn do tác dụng dần dần của nó. Chúng tôi thảo luận chi tiết về BTVA trong bài biên tập này. CÁC LĨNH VỰC ĐƯỢC BAO QUÁT Chúng tôi thảo luận chi tiết về kinh nghiệm của mình với BTVA bao gồm lựa chọn bệnh nhân, thiết bị, quy trình, chăm sóc sau thủ thuật và các biến chứng. Chúng tôi cũng xem xét tài liệu để xác định ưu và nhược điểm khi sử dụng nó. Các phương thức khác như van nội phế quản, cuộn dây và chất bịt kín phổi cũng được thảo luận ngắn gọn. Ý KIẾN CỦA CHUYÊN GIA Phẫu thuật cắt bỏ hơi là một phương pháp mới và an toàn để gây giảm thể tích phổi ở những bệnh nhân bị khí phế thũng. Các tác dụng là dần dần, do đó có khả năng an toàn hơn các phương thức xâm lấn tối thiểu khác. Viêm phổi và nhiễm trùng là những tác dụng phụ phổ biến. Cũng như các kỹ thuật BLVR khác, cần phải đánh giá từng trường hợp để xác định ứng viên phù hợp cho BTVA. Cần có thêm các nghiên cứu lớn hơn trước khi BTVA trở thành tiêu chuẩn chăm sóc trong điều trị bệnh nhân bị khí phế thũng."} +{"idx": 1870818, "en": "Natural highly fecund populations abound. These range from viruses to gadids. Many highly fecund populations are economically important. Highly fecund populations provide an important contrast to the low-fecundity organisms that have traditionally been applied in evolutionary studies. A key question regarding high fecundity is whether large numbers of offspring are produced on a regular basis, by few individuals each time, in a sweepstakes mode of reproduction. Such reproduction characteristics are not incorporated into the classical Wright-Fisher model, the standard reference model of population genetics, or similar types of models, in which each individual can produce only small numbers of offspring relative to the population size. The expected genomic footprints of population genetic models of sweepstakes reproduction are very different from those of the Wright-Fisher model. A key, immediate issue involves identifying the footprints of sweepstakes reproduction in genomic data. Whole-genome sequencing data can be used to distinguish the patterns made by sweepstakes reproduction from the patterns made by population growth in a population evolving according to the Wright-Fisher model (or similar models). If the hypothesis of sweepstakes reproduction cannot be rejected, then models of sweepstakes reproduction and associated multiple-merger coalescents will become at least as relevant as the Wright-Fisher model (or similar models) and the Kingman coalescent, the cornerstones of mathematical population genetics, in further discussions of evolutionary genomics of highly fecund populations.", "gemini": "Các quần thể tự nhiên có khả năng sinh sản cao rất nhiều. Chúng dao động từ vi-rút đến cá tuyết. Nhiều quần thể có khả năng sinh sản cao có tầm quan trọng về mặt kinh tế. Các quần thể có khả năng sinh sản cao tạo nên sự tương phản quan trọng với các sinh vật có khả năng sinh sản thấp vốn thường được áp dụng trong các nghiên cứu tiến hóa. Một câu hỏi chính liên quan đến khả năng sinh sản cao là liệu một số lượng lớn con cái có được sinh ra một cách thường xuyên, bởi một số ít cá thể mỗi lần, theo chế độ sinh sản rút thăm không. Các đặc điểm sinh sản như vậy không được đưa vào mô hình Wright-Fisher cổ điển, mô hình tham chiếu tiêu chuẩn về di truyền quần thể hoặc các loại mô hình tương tự, trong đó mỗi cá thể chỉ có thể sinh ra một số lượng nhỏ con cái so với quy mô quần thể. Dấu vết bộ gen dự kiến của các mô hình di truyền quần thể về sinh sản rút thăm rất khác so với mô hình Wright-Fisher. Một vấn đề cấp bách chính liên quan đến việc xác định dấu vết của sinh sản rút thăm trong dữ liệu bộ gen. Dữ liệu giải trình tự toàn bộ bộ gen có thể được sử dụng để phân biệt các kiểu do sinh sản rút thăm tạo ra khỏi các kiểu do sự gia tăng dân số trong một quần thể tiến hóa theo mô hình Wright-Fisher (hoặc các mô hình tương tự). Nếu không thể bác bỏ giả thuyết về sinh sản rút thăm, thì các mô hình sinh sản rút thăm và các hợp nhất nhiều lần liên quan sẽ trở nên ít nhất là có liên quan như mô hình Wright-Fisher (hoặc các mô hình tương tự) và hợp nhất Kingman, nền tảng của di truyền quần thể toán học, trong các cuộc thảo luận tiếp theo về bộ gen tiến hóa của các quần thể có khả năng sinh sản cao."} +{"idx": 1870819, "en": "Cells utilize transcriptional and posttranscriptional mechanisms to alter gene expression in response to environmental cues. Gene-specific controls, including changing the translation of specific messenger RNAs (mRNAs), provide a rapid means to respond precisely to different conditions. Upstream open reading frames (uORFs) are known to control the translation of mRNAs. Recent studies in bacteria and eukaryotes have revealed the functions of evolutionarily conserved uORF-encoded peptides. Some of these uORF-encoded nascent peptides enable responses to specific metabolites to modulate the translation of their mRNAs by stalling ribosomes and through ribosome stalling may also modulate the level of their mRNAs. In this review, we highlight several examples of conserved uORF nascent peptides that stall ribosomes to regulate gene expression in response to specific metabolites in bacteria, fungi, mammals, and plants.", "gemini": "Các tế bào sử dụng các cơ chế phiên mã và sau phiên mã để thay đổi biểu hiện gen nhằm đáp ứng các tín hiệu môi trường. Các cơ chế kiểm soát đặc hiệu gen, bao gồm thay đổi quá trình dịch mã các ARN thông tin (mARN) đặc hiệu, cung cấp một phương tiện nhanh chóng để phản ứng chính xác với các điều kiện khác nhau. Các khung đọc mở đầu (uORF) được biết là kiểm soát quá trình dịch mã của mARN. Các nghiên cứu gần đây trên vi khuẩn và eukaryote đã tiết lộ các chức năng của các peptide được mã hóa bởi uORF có sự bảo tồn theo tiến hóa. Một số peptide mới được mã hóa bởi uORF này cho phép phản ứng với các chất chuyển hóa cụ thể để điều chỉnh quá trình dịch mã mARN của chúng bằng cách làm ribosome dừng lại và thông qua việc ribosome dừng lại cũng có thể điều chỉnh mức độ mARN của chúng. Trong bài tổng quan này, chúng tôi nêu bật một số ví dụ về các peptide mới được mã hóa bởi uORF bảo tồn làm ribosome dừng lại để điều chỉnh biểu hiện gen nhằm đáp ứng các chất chuyển hóa cụ thể ở vi khuẩn, nấm, động vật có vú và thực vật."} +{"idx": 1870820, "en": "Uncovering the genes, variants, and interactions underlying crop diversity is a frontier in plant genetics. Phenotypic variation often does not reflect the cumulative effect of individual gene mutations. This deviation is due to epistasis, in which interactions between alleles are often unpredictable and quantitative in effect. Recent advances in genomics and genome-editing technologies are elevating the study of epistasis in crops. Using the traits and developmental pathways that were major targets in domestication and breeding, we highlight how epistasis is central in guiding the behavior of the genetic variation that shapes quantitative trait variation. We outline new strategies that illuminate how quantitative epistasis from modified gene dosage defines background dependencies. Advancing our understanding of epistasis in crops can reveal new principles and approaches to engineering targeted improvements in agriculture.", "gemini": "Việc khám phá các gen, biến thể và tương tác nằm bên dưới sự đa dạng của cây trồng là một lĩnh vực tiên tiến trong di truyền học thực vật. Biến dị kiểu hình thường không phản ánh hiệu ứng tích lũy của các đột biến gen riêng lẻ. Sự sai lệch này là do hiện tượng tương tác gen, trong đó các tương tác giữa các alen thường không thể đoán trước và có hiệu ứng về mặt số lượng. Những tiến bộ gần đây trong công nghệ chỉnh sửa gen và gen đã nâng cao nghiên cứu về tương tác gen ở cây trồng. Sử dụng các đặc điểm và con đường phát triển là các mục tiêu chính trong thuần hóa và lai tạo, chúng tôi nêu bật cách tương tác gen đóng vai trò trung tâm trong việc định hướng hành vi của biến dị di truyền định hình biến dị về các đặc điểm định lượng. Chúng tôi phác thảo các chiến lược mới làm sáng tỏ cách tương tác gen định lượng từ liều lượng gen đã sửa đổi xác định sự phụ thuộc vào nền. Việc thúc đẩy sự hiểu biết của chúng ta về tương tác gen ở cây trồng có thể tiết lộ các nguyên tắc và phương pháp mới để cải tiến mục tiêu trong nông nghiệp."} +{"idx": 1870821, "en": "The single gene, single protein, single function hypothesis is increasingly becoming obsolete. Numerous studies have demonstrated that individual proteins can moonlight, meaning they can have multiple functions based on their cellular or developmental context. In this review, we discuss moonlighting proteins, highlighting the biological pathways where this phenomenon may be particularly relevant. In addition, we combine genetic, cell biological, and evolutionary perspectives so that we can better understand how, when, and why moonlighting proteins may take on multiple roles.", "gemini": "Giả thuyết một gen, một protein, một chức năng ngày càng trở nên lỗi thời. Nhiều nghiên cứu đã chứng minh rằng các protein riêng lẻ có thể hoạt động nhiều chức năng, nghĩa là chúng có thể có nhiều chức năng dựa trên bối cảnh tế bào hoặc phát triển của chúng. Trong bài tổng quan này, chúng tôi thảo luận về các protein hoạt động nhiều chức năng, nêu bật các con đường sinh học mà hiện tượng này có thể đặc biệt phù hợp. Ngoài ra, chúng tôi kết hợp các quan điểm di truyền, sinh học tế bào và tiến hóa để có thể hiểu rõ hơn về cách thức, thời điểm và lý do khiến các protein hoạt động nhiều chức năng có thể đảm nhận nhiều vai trò."} +{"idx": 1870822, "en": "Recent advances in pseudouridine detection reveal a complex pseudouridine landscape that includes messenger RNA and diverse classes of noncoding RNA in human cells. The known molecular functions of pseudouridine, which include stabilizing RNA conformations and destabilizing interactions with varied RNA-binding proteins, suggest that RNA pseudouridylation could have widespread effects on RNA metabolism and gene expression. Here, we emphasize how much remains to be learned about the RNA targets of human pseudouridine synthases, their basis for recognizing distinct RNA sequences, and the mechanisms responsible for regulated RNA pseudouridylation. We also examine the roles of noncoding RNA pseudouridylation in splicing and translation and point out the potential effects of mRNA pseudouridylation on protein production, including in the context of therapeutic mRNAs.", "gemini": "Những tiến bộ gần đây trong việc phát hiện pseudouridine cho thấy một bối cảnh pseudouridine phức tạp bao gồm RNA thông tin và nhiều loại RNA không mã hóa khác nhau trong tế bào người. Các chức năng phân tử đã biết của pseudouridine, bao gồm ổn định cấu trúc RNA và làm mất ổn định các tương tác với nhiều loại protein liên kết RNA, cho thấy rằng pseudouridylation RNA có thể có tác động rộng rãi đến quá trình chuyển hóa RNA và biểu hiện gen. Ở đây, chúng tôi nhấn mạnh rằng vẫn còn nhiều điều cần tìm hiểu về các mục tiêu RNA của pseudouridine synthase ở người, cơ sở của chúng để nhận ra các trình tự RNA riêng biệt và các cơ chế chịu trách nhiệm cho quá trình pseudouridylation RNA được điều chỉnh. Chúng tôi cũng xem xét vai trò của pseudouridylation RNA không mã hóa trong quá trình cắt nối và dịch mã và chỉ ra các tác động tiềm ẩn của pseudouridylation mRNA đối với quá trình sản xuất protein, bao gồm trong bối cảnh mRNA điều trị."} +{"idx": 1870823, "en": "To investigate the transfer of pyrrolizidine alkaloids (PAs) from feed to milk, rumen-cannulated dairy cows were intra-ruminally fed with 200 g/day of dried plant material of either ragwort (mixture of Jacobaea vulgaris and Senecio inaequidens), common groundsel (Senecio vulgaris) or viper's bugloss (Echium vulgare) for a period of 4 days. PA levels in the plant materials were 3767, 2792 and 1674 µg g-1 respectively. Feed intake, milk yield and several blood parameters indicative for liver function were not influenced by the treatment. When fed ragwort, increased levels of PAs were detected in the milk, in particular jacoline and an unidentified cyclic diester, possibly a hydroxylated metabolite from retrorsine. The latter was the most important PA in milk from cows fed common groundsel. For viper's bugloss, echimidine was the most abundant identified PA but in addition several hydroxylated PA metabolites were detected. For ragwort, the overall PA transfer was estimated at 0.05% and 1.4% for jacoline (N-oxide). Transfer rates were similar for viper's bugloss (0.05%) but lower for common groundsel (0.01%). Only a small portion of the administered PAs was quantified in milk, urine and faeces, with an overall balance of 4.5%, 2.9% and 5.8%, for ragwort, common groundsel and viper's bugloss, respectively. Samples taken from the rumen indicated that the N-oxides were converted into the free bases, which was confirmed by in vitro studies with the same plant species incubated with ruminal fluid. These results confirm that the transfer of PAs to milk is relatively low but may be of concern for human health regarding the genotoxic and carcinogenic properties of these compounds. The transfer rate depends on the type of PAs present in the weeds. The incomplete balance of input vs output stresses the need to further investigate the metabolism and the potential transfer of metabolites into edible products.", "gemini": "Để nghiên cứu quá trình chuyển hóa các alkaloid pyrrolizidine (PA) từ thức ăn sang sữa, những con bò sữa có ống thông dạ cỏ được cho ăn trong dạ cỏ 200 g/ngày vật liệu thực vật khô của cây hoắc hương (hỗn hợp Jacobaea vulgaris và Senecio inaequidens), cây rau diếp xoăn thông thường (Senecio vulgaris) hoặc cây lưỡi rắn (Echium vulgare) trong thời gian 4 ngày. Hàm lượng PA trong các vật liệu thực vật lần lượt là 3767, 2792 và 1674 µg g-1. Lượng thức ăn nạp vào, lượng sữa sản xuất và một số thông số máu biểu thị chức năng gan không bị ảnh hưởng bởi quá trình điều trị. Khi cho ăn cây hoắc hương, người ta phát hiện thấy hàm lượng PA tăng trong sữa, đặc biệt là jacoline và một dieste vòng chưa xác định, có thể là chất chuyển hóa hydroxyl hóa từ retrorsine. Chất sau là PA quan trọng nhất trong sữa từ những con bò ăn rau diếp xoăn thông thường. Đối với cây lưỡi rắn, echimidine là PA được xác định nhiều nhất nhưng ngoài ra còn phát hiện thấy một số chất chuyển hóa PA hydroxyl hóa. Đối với cây hoắc hương, tổng lượng PA chuyển hóa ước tính là 0,05% và 1,4% đối với jacoline (N-oxide). Tỷ lệ chuyển hóa tương tự đối với cây lưỡi rắn (0,05%) nhưng thấp hơn đối với cây rau diếp xoăn thông thường (0,01%). Chỉ một phần nhỏ PA được đưa vào được định lượng trong sữa, nước tiểu và phân, với tổng số cân bằng là 4,5%, 2,9% và 5,8%, tương ứng đối với cây hoắc hương, cây rau diếp xoăn thông thường và cây lưỡi rắn. Các mẫu lấy từ dạ cỏ cho thấy N-oxide đã được chuyển hóa thành các bazơ tự do, điều này đã được xác nhận bằng các nghiên cứu trong ống nghiệm với cùng loài thực vật được ủ với dịch dạ cỏ. Những kết quả này xác nhận rằng quá trình chuyển hóa PA thành sữa tương đối thấp nhưng có thể gây lo ngại cho sức khỏe con người liên quan đến các đặc tính gây độc gen và gây ung thư của các hợp chất này. Tỷ lệ chuyển hóa phụ thuộc vào loại PA có trong cỏ dại. Sự mất cân bằng giữa đầu vào và đầu ra nhấn mạnh sự cần thiết phải tiếp tục nghiên cứu quá trình chuyển hóa và khả năng chuyển hóa các chất chuyển hóa thành các sản phẩm ăn được."} +{"idx": 1870824, "en": "BACKGROUND Hemorrhage is one of the leading causes of preventable death in both military and civilian trauma. Implementation of items such as tourniquets and hemostatic dressings are helpful in controlling hemorrhage and increasing the survival rate of casualties when such injuries occur. Prehospital blood transfusions are used to treat patients with severe injuries where the standard methods of hemorrhage control are not an effective form of treatment. There is limited research and no widely accepted protocol on pediatric prehospital blood transfusions. METHODS We queried the Department of Defense Trauma Registry (DODTR) for all pediatric subjects admitted to U.S. and Coalition fixed-facility hospitals in Iraq and Afghanistan from January 2007 to January 2016. This is a secondary analysis of casualties that received blood products prehospital. RESULTS From January 2007 through January 2016 there were 3439 pediatric casualties within the registry. Within this group, 22 casualties that received one or more blood product prehospital were identified. Children in the 10-14 years age (40%) group made up the largest proportion, 86% were male, almost all were injured by explosive (63%) or firearm (27%), and 77% survived to hospital discharge. The most frequently administered blood product was packed red cells (n = 17). Of the 22, 15 underwent massive transfusion within the first 24 hours of admission. CONCLUSIONS Prehospital administration of blood products occurred infrequently within this pediatric dataset, but those that received blood were critically injured with most receiving a massive transfusion. Given the frequency with which medical personnel are carrying blood products in the prehospital, combat setting, guidelines specific to pediatric administration would be beneficial.", "gemini": "BỐI CẢNH Xuất huyết là một trong những nguyên nhân hàng đầu gây tử vong có thể phòng ngừa được ở cả chấn thương quân sự và dân sự. Việc triển khai các vật dụng như garô và băng cầm máu rất hữu ích trong việc kiểm soát xuất huyết và tăng tỷ lệ sống sót của thương vong khi xảy ra những chấn thương như vậy. Truyền máu trước viện được sử dụng để điều trị bệnh nhân bị thương nặng khi các phương pháp kiểm soát xuất huyết tiêu chuẩn không phải là hình thức điều trị hiệu quả. Có rất ít nghiên cứu và không có phác đồ nào được chấp nhận rộng rãi về truyền máu trước viện cho trẻ em. PHƯƠNG PHÁP Chúng tôi đã truy vấn Cơ quan đăng ký chấn thương của Bộ Quốc phòng (DODTR) về tất cả các đối tượng trẻ em được đưa vào các bệnh viện cố định của Hoa Kỳ và Liên minh tại Iraq và Afghanistan từ tháng 1 năm 2007 đến tháng 1 năm 2016. Đây là phân tích thứ cấp đối với những thương vong đã nhận được các chế phẩm máu trước viện. KẾT QUẢ Từ tháng 1 năm 2007 đến tháng 1 năm 2016 có 3439 thương vong ở trẻ em trong sổ đăng ký. Trong nhóm này, đã xác định được 22 trường hợp thương vong nhận được một hoặc nhiều chế phẩm máu trước viện. Trẻ em trong nhóm tuổi 10-14 (40%) chiếm tỷ lệ lớn nhất, 86% là nam, hầu hết đều bị thương do nổ (63%) hoặc súng (27%) và 77% sống sót để xuất viện. Chế phẩm máu được sử dụng thường xuyên nhất là hồng cầu đóng gói (n = 17). Trong số 22 trường hợp, 15 trường hợp đã được truyền máu ồ ạt trong vòng 24 giờ đầu nhập viện. KẾT LUẬN Việc sử dụng các chế phẩm máu trước viện xảy ra không thường xuyên trong tập dữ liệu về nhi khoa này, nhưng những người nhận máu bị thương nặng và hầu hết đều được truyền máu ồ ạt. Với tần suất mà nhân viên y tế mang theo các chế phẩm máu trong bối cảnh chiến đấu trước viện, các hướng dẫn cụ thể cho việc sử dụng cho trẻ em sẽ rất có lợi."} +{"idx": 1870825, "en": "PURPOSES To describe the prevalence of ocular features among COVID-19 patients and their relationship with clinical data, inflammatory markers and respiratory support therapy (including CPAP); to investigate SARS-CoV-2 in ocular secretions of symptomatic patients. METHODS 172 COVID-19 patients were evaluated for presence of ocular manifestations. Clinical and laboratory data were also reviewed. Conjunctival swabs were analyzed for SARS-CoV-2 by RT-PCR. RESULTS Forty-five patients (26.2%) reported ocular manifestations. Patients treated with CPAP were more likely to have ocular abnormalities (p <.01). The presence of ocular symptoms was not associated with more significant alterations on blood tests. Conjunctival swabs from patients with suspect conjunctivitis yielded negative results for SARS-CoV-2. CONCLUSIONS Ocular features are not infrequent in COVID-19 patients, but the presence of SARS-CoV-2 in ocular secretions is low. Ocular manifestations in hospitalized COVID-19 patients can also be a consequence of respiratory support therapy. Prevention of possible transmission through ocular secretions is still recommended.", "gemini": "MỤC ĐÍCH Mô tả tỷ lệ mắc các đặc điểm về mắt ở những bệnh nhân mắc COVID-19 và mối quan hệ của chúng với dữ liệu lâm sàng, các dấu hiệu viêm và liệu pháp hỗ trợ hô hấp (bao gồm CPAP); để nghiên cứu SARS-CoV-2 trong dịch tiết mắt của những bệnh nhân có triệu chứng. PHƯƠNG PHÁP 172 bệnh nhân COVID-19 được đánh giá về sự hiện diện của các biểu hiện về mắt. Dữ liệu lâm sàng và xét nghiệm cũng được xem xét. Tăm dịch kết mạc được phân tích để tìm SARS-CoV-2 bằng RT-PCR. KẾT QUẢ Bốn mươi lăm bệnh nhân (26,2%) báo cáo các biểu hiện về mắt. Những bệnh nhân được điều trị bằng CPAP có nhiều khả năng gặp các bất thường về mắt (p <.01). Sự hiện diện của các triệu chứng về mắt không liên quan đến những thay đổi đáng kể hơn trong các xét nghiệm máu. Tăm dịch kết mạc từ những bệnh nhân nghi ngờ viêm kết mạc cho kết quả âm tính với SARS-CoV-2. KẾT LUẬN Các đặc điểm về mắt không phải là không phổ biến ở những bệnh nhân mắc COVID-19, nhưng sự hiện diện của SARS-CoV-2 trong dịch tiết mắt là thấp. Các biểu hiện về mắt ở những bệnh nhân COVID-19 nhập viện cũng có thể là hậu quả của liệu pháp hỗ trợ hô hấp. Việc phòng ngừa lây truyền có thể xảy ra thông qua dịch tiết mắt vẫn được khuyến cáo."} +{"idx": 1870826, "en": "OBJECTIVE To describe the ocular manifestations in a case of novel coronavirus disease 2019 (COVID-19). MATERIAL AND METHODS A case of unilateral panuveitis and optic neuritis as initial presentation of COVID-19. RESULTS As it is published, angiotensin-converting-enzyme-2 receptors can be found in many organs, such as the eyes, nerves, and vessels, so extrapulmonary involvement would be expected. According to current evidence and clinical characteristics of the patient, uveitis and optic neuritis could be produced by the virus. CONCLUSIONS It is fundamental to consider panuveitis and optic neuritis as an unusual presentation of ocular involvement in COVID-19 so proper care can be given to the patients.", "gemini": "MỤC TIÊU Mô tả các biểu hiện về mắt trong một trường hợp mắc bệnh do chủng vi-rút corona mới 2019 (COVID-19). VẬT LIỆU VÀ PHƯƠNG PHÁP Một trường hợp viêm toàn bộ màng bồ đào đơn phương và viêm dây thần kinh thị giác là biểu hiện ban đầu của COVID-19. KẾT QUẢ Theo như đã công bố, thụ thể men chuyển đổi angiotensin-2 có thể được tìm thấy ở nhiều cơ quan, chẳng hạn như mắt, dây thần kinh và mạch máu, do đó có thể dự đoán được tình trạng liên quan đến ngoài phổi. Theo các bằng chứng hiện tại và các đặc điểm lâm sàng của bệnh nhân, viêm màng bồ đào và viêm dây thần kinh thị giác có thể do vi-rút gây ra. KẾT LUẬN Điều cơ bản là phải xem viêm toàn bộ màng bồ đào và viêm dây thần kinh thị giác là một biểu hiện bất thường của tình trạng liên quan đến mắt trong COVID-19 để có thể chăm sóc bệnh nhân đúng cách."} +{"idx": 1870827, "en": "A method for the determination of deoxynivalenol (DON) and its major conjugates in cereals was developed including an immunoaffinity column (IAC) clean-up coupled in-line with high-performance liquid chromatography, post-column derivatisation and fluorescence detection. An IAC for DON with cross-reactivity to 15-AcDON, 3-AcDON and DON-3-G enabled this approach. The isolated analytes were introduced into the chromatographic system without aliquotation employing the hot water elution technique, resulting in the desired low LOQ values for monitoring these analytes in cereals. The absence of any organic solvent during sample preparation in combination with an in-line IAC clean-up renders the method simple, fast, and environmentally friendly. Special attention was paid to inherent IACs properties such as cross-reactivity, analytes' competition and capacity. The method was applied to determine DON and its major conjugates in barley, wheat and maize in the range of 10-1000 µg kg-1 of DON, 10-300 µg kg-1 of DON-3-G and 15-AcDON and 10-100 µg kg-1 of 3-AcDON. The apparent recoveries varied from 87% to 110% (average of 98%) and the intermediate precision was below 13.5% RSD (except for DON-3-G in wheat). Fifteen maize, wheat and barley samples were analysed revealing levels of DON conjugates that accounted from 9% to 60% of the \"total DON\" content (m/m). In general, the frequency and the measured mass fractions decreased in the following order: DON>DON-3-G>15-AcDON>3-AcDON.", "gemini": "Một phương pháp xác định deoxynivalenol (DON) và các liên hợp chính của nó trong ngũ cốc đã được phát triển bao gồm làm sạch cột miễn dịch (IAC) kết hợp trực tuyến với sắc ký lỏng hiệu năng cao, dẫn xuất sau cột và phát hiện huỳnh quang. Một IAC dành cho DON có phản ứng chéo với 15-AcDON, 3-AcDON và DON-3-G đã cho phép phương pháp tiếp cận này. Các chất phân tích được phân lập được đưa vào hệ thống sắc ký mà không cần định lượng bằng kỹ thuật giải hấp bằng nước nóng, dẫn đến các giá trị LOQ thấp mong muốn để theo dõi các chất phân tích này trong ngũ cốc. Việc không có bất kỳ dung môi hữu cơ nào trong quá trình chuẩn bị mẫu kết hợp với việc làm sạch IAC trực tuyến giúp phương pháp này trở nên đơn giản, nhanh chóng và thân thiện với môi trường. Đặc biệt chú ý đến các tính chất vốn có của IAC như phản ứng chéo, sự cạnh tranh và khả năng của các chất phân tích. Phương pháp này được áp dụng để xác định DON và các liên hợp chính của nó trong lúa mạch, lúa mì và ngô trong phạm vi 10-1000 µg kg-1 của DON, 10-300 µg kg-1 của DON-3-G và 15-AcDON và 10-100 µg kg-1 của 3-AcDON. Độ thu hồi biểu kiến dao động từ 87% đến 110% (trung bình là 98%) và độ chính xác trung gian dưới 13,5% RSD (trừ DON-3-G trong lúa mì). Mười lăm mẫu ngô, lúa mì và lúa mạch đã được phân tích cho thấy mức độ liên hợp DON chiếm từ 9% đến 60% tổng hàm lượng \"DON\" (m/m). Nhìn chung, tần suất và phân số khối lượng đo được giảm dần theo thứ tự sau: DON>DON-3-G>15-AcDON>3-AcDON."} +{"idx": 1870828, "en": "OBJECTIVE To evaluate the validity and reliability of the Fonseca anamnestic index (FAI) over time in Turkish females. METHODS Four hundred-fourteen patients who visited the otorhinolaryngology clinic were initially included in the study between January 1, 2018 and January 1, 2019. The Turkish version of the FAI is a self-report instrument that was completed by all of the patients after a brief explanation from the clinician. Cronbach's alpha was calculated using data from the first questionnaire. RESULTS The results of the first assessment showed 243 patients had no TMD (58.7%), 40 had mild TMD (9.7%), 87 had moderate TMD (21%), and 44 had severe TMD (10.6%). At the second assessment, the respective values were 243 (58.7%), 37 (8.9%), 98 (23.7%), and 36 (8.7%). The internal consistency of the first test was high. CONCLUSION The FAI can be used to collect a large amount of data easily, at minimal cost.", "gemini": "MỤC TIÊU Đánh giá độ tin cậy và giá trị của chỉ số bệnh sử Fonseca (FAI) theo thời gian ở phụ nữ Thổ Nhĩ Kỳ. PHƯƠNG PHÁP Bốn trăm mười bốn bệnh nhân đến khám tại phòng khám tai mũi họng ban đầu được đưa vào nghiên cứu trong khoảng thời gian từ ngày 1 tháng 1 năm 2018 đến ngày 1 tháng 1 năm 2019. Phiên bản tiếng Thổ Nhĩ Kỳ của FAI là một công cụ tự báo cáo được tất cả bệnh nhân hoàn thành sau một lời giải thích ngắn gọn từ bác sĩ lâm sàng. Độ tin cậy của Cronbach được tính toán bằng cách sử dụng dữ liệu từ bảng câu hỏi đầu tiên. KẾT QUẢ Kết quả đánh giá đầu tiên cho thấy 243 bệnh nhân không mắc RMD (58,7%), 40 bệnh nhân mắc RMD nhẹ (9,7%), 87 bệnh nhân mắc RMD vừa (21%) và 44 bệnh nhân mắc RMD nặng (10,6%). Ở lần đánh giá thứ hai, các giá trị tương ứng là 243 (58,7%), 37 (8,9%), 98 (23,7%) và 36 (8,7%). Độ tin cậy nội bộ của bài kiểm tra đầu tiên là cao. KẾT LUẬN FAI có thể được sử dụng để thu thập một lượng lớn dữ liệu một cách dễ dàng, với chi phí tối thiểu."} +{"idx": 1870829, "en": "Systemic inflammation is associated with obesity and chronic disease risk. Intake of dairy foods is associated with reduced risk of type 2 diabetes and cardiovascular disease; however, the impact of dairy foods on inflammation is not well-established. The objective of this study was to conduct a systematic review to evaluate the effect of dairy product (milk, cheese, and yogurt) and dairy protein consumption on low-grade systemic inflammation in adults without severe inflammatory disorders. A literature search was completed in September 2019 using PubMed and CENTRAL as well as inspection of reference lists from relevant review articles. The search resulted in the identification of 27 randomized controlled trials which were included in this analysis. In the 19 trials which evaluated dairy products, 10 reported no effect of the intervention, while 8 reported a reduction in at least one biomarker of inflammation. All 8 trials that investigated dairy protein intake on markers of inflammation reported no effect of the intervention. The available literature suggests that dairy products and dairy proteins have neutral to beneficial effects on biomarkers of inflammation. Additional clinical studies designed using inflammatory biomarkers as the primary outcome are needed to fully elucidate the effects of dairy intake on inflammation.", "gemini": "Viêm hệ thống có liên quan đến béo phì và nguy cơ mắc bệnh mãn tính. Tiêu thụ các sản phẩm từ sữa có liên quan đến việc giảm nguy cơ mắc bệnh tiểu đường tuýp 2 và bệnh tim mạch; tuy nhiên, tác động của các sản phẩm từ sữa đối với tình trạng viêm vẫn chưa được thiết lập rõ ràng. Mục tiêu của nghiên cứu này là tiến hành một đánh giá có hệ thống để đánh giá tác động của sản phẩm từ sữa (sữa, pho mát và sữa chua) và tiêu thụ protein từ sữa đối với tình trạng viêm hệ thống nhẹ ở những người trưởng thành không mắc các rối loạn viêm nghiêm trọng. Một tìm kiếm tài liệu đã được hoàn thành vào tháng 9 năm 2019 bằng cách sử dụng PubMed và CENTRAL cũng như kiểm tra danh sách tham chiếu từ các bài báo tổng quan có liên quan. Cuộc tìm kiếm đã xác định được 27 thử nghiệm có đối chứng ngẫu nhiên được đưa vào phân tích này. Trong số 19 thử nghiệm đánh giá các sản phẩm từ sữa, 10 báo cáo không có tác dụng của sự can thiệp, trong khi 8 báo cáo giảm ít nhất một sinh học chỉ điểm của tình trạng viêm. Tất cả 8 thử nghiệm điều tra lượng protein từ sữa trên các dấu ấn của tình trạng viêm đều báo cáo không có tác dụng của sự can thiệp. Các tài liệu hiện có cho thấy các sản phẩm từ sữa và protein từ sữa có tác dụng trung tính đến có lợi đối với các sinh học chỉ điểm của tình trạng viêm. Cần có thêm các nghiên cứu lâm sàng được thiết kế sử dụng các sinh học chỉ điểm viêm làm kết quả chính để làm sáng tỏ đầy đủ các tác động của việc tiêu thụ sữa đối với tình trạng viêm."} +{"idx": 1870830, "en": "Aggrecan is a large proteoglycan that forms giant hydrated aggregates with hyaluronan in the extracellular matrix (ECM). The extraordinary resistance of these aggregates to compression explains their abundance in articular cartilage of joints where they ensure adequate load-bearing. In the brain, they provide mechanical buffering and contribute to formation of perineuronal nets, which regulate synaptic plasticity. Aggrecan is also present in cardiac jelly, developing heart valves, and blood vessels during cardiovascular development. Whereas aggrecan is essential for skeletal development, its function in the developing cardiovascular system remains to be fully elucidated. An excess of aggrecan was demonstrated in cardiovascular tissues in aortic aneurysms, atherosclerosis, vascular re-stenosis after injury, and varicose veins. It is a product of vascular smooth muscle and is likely to be an important component of pericellular matrix, where its levels are regulated by proteases. Aggrecan can contribute to specific biophysical and regulatory properties of cardiovascular ECM via the diverse interactions of its domains, and its accumulation is likely to have a significant role in developmental and disease pathways. Here, the established biological functions of aggrecan, its cardiovascular associations, and potential roles in cardiovascular development and disease are discussed.", "gemini": "Aggrecan là một proteoglycan lớn tạo thành các tập hợp hydrat khổng lồ với hyaluronan trong ma trận ngoại bào (ECM). Sức đề kháng phi thường của các tập hợp này đối với lực nén giải thích sự phong phú của chúng trong sụn khớp của các khớp, nơi chúng đảm bảo khả năng chịu tải đầy đủ. Trong não, chúng cung cấp khả năng đệm cơ học và góp phần hình thành các mạng lưới quanh nơron, điều chỉnh tính dẻo của khớp thần kinh. Aggrecan cũng có mặt trong thạch tim, phát triển van tim và mạch máu trong quá trình phát triển tim mạch. Trong khi aggrecan rất cần thiết cho sự phát triển của xương, thì chức năng của nó trong hệ thống tim mạch đang phát triển vẫn chưa được làm sáng tỏ đầy đủ. Một lượng aggrecan dư thừa đã được chứng minh trong các mô tim mạch ở phình động mạch chủ, xơ vữa động mạch, hẹp lại mạch máu sau chấn thương và giãn tĩnh mạch. Đây là sản phẩm của cơ trơn mạch máu và có khả năng là một thành phần quan trọng của ma trận quanh tế bào, nơi nồng độ của nó được điều chỉnh bởi các protease. Aggrecan có thể góp phần vào các đặc tính sinh học và điều hòa cụ thể của ECM tim mạch thông qua các tương tác đa dạng của các miền của nó, và sự tích tụ của nó có khả năng đóng vai trò quan trọng trong các con đường phát triển và bệnh tật. Tại đây, các chức năng sinh học đã được thiết lập của aggrecan, các liên kết tim mạch của nó và các vai trò tiềm ẩn trong quá trình phát triển và bệnh tim mạch được thảo luận."} +{"idx": 1870831, "en": "The purpose of this article is to examine hospital social responsibility practices using a content analysis method. It selected a sample of 522 hospitals in U.S. and collected data from hospitals' official websites. Significant emphasis is found to be placed on CSR practices associated with marketplace activities that are mostly stakeholder-driven. Non-profit hospitals had stronger awareness on the actions of being a socially responsible organization. The results also confirm significant relationship between active CSR programs and hospital. The findings shed light on hospital social responsibility and provides a unifying conceptual basis that explains how hospitals in U.S execute CSR programs.", "gemini": "Mục đích của bài viết này là xem xét các hoạt động trách nhiệm xã hội của bệnh viện bằng phương pháp phân tích nội dung. Bài viết đã chọn một mẫu gồm 522 bệnh viện tại Hoa Kỳ và thu thập dữ liệu từ các trang web chính thức của bệnh viện. Nhấn mạnh đáng kể được đặt vào các hoạt động CSR liên quan đến các hoạt động trên thị trường chủ yếu do các bên liên quan thúc đẩy. Các bệnh viện phi lợi nhuận có nhận thức mạnh mẽ hơn về các hành động của một tổ chức có trách nhiệm xã hội. Kết quả cũng xác nhận mối quan hệ đáng kể giữa các chương trình CSR tích cực và bệnh viện. Những phát hiện làm sáng tỏ trách nhiệm xã hội của bệnh viện và cung cấp một cơ sở khái niệm thống nhất giải thích cách các bệnh viện ở Hoa Kỳ thực hiện các chương trình CSR."} +{"idx": 1870832, "en": "To examine race, gender, and alcohol use level as moderators of the association between protective behavioral strategies (PBS) and alcohol-related problems. Participants: A sample of 12,011 participants who reported recent drinking (87.7% White, 61% Women) from Project INTEGRATE, a study that combined individual participant data (IPD) from 24 brief motivational intervention trials for college students. Methods: Hierarchical regressions were conducted to determine whether there was a moderated effect of PBS on alcohol problems across alcohol use levels, and whether the moderated protective effect of PBS by alcohol use differed by gender and race. Results: The protective association between PBS and alcohol-related problems was greater for those who drank less. This moderated effect did not differ across men and women or across racial groups. Conclusions: College drinking prevention programs should ensure that students are aware of the limits of PBS as a mitigator of alcohol problems.", "gemini": "Kiểm tra chủng tộc, giới tính và mức độ sử dụng rượu như những yếu tố điều chỉnh mối liên quan giữa các chiến lược hành vi bảo vệ (PBS) và các vấn đề liên quan đến rượu. Người tham gia: Một mẫu gồm 12.011 người tham gia đã báo cáo uống rượu gần đây (87,7% người da trắng, 61% phụ nữ) từ Dự án INTEGRATE, một nghiên cứu kết hợp dữ liệu người tham gia cá nhân (IPD) từ 24 thử nghiệm can thiệp động lực ngắn hạn cho sinh viên đại học. Phương pháp: Các hồi quy phân cấp đã được tiến hành để xác định xem có hiệu ứng điều chỉnh của PBS đối với các vấn đề về rượu trên các mức độ sử dụng rượu hay không và liệu hiệu ứng bảo vệ điều chỉnh của PBS theo mức độ sử dụng rượu có khác nhau giữa nam và nữ hay không. chủng tộc. Kết quả: Mối liên hệ bảo vệ giữa PBS và các vấn đề liên quan đến rượu lớn hơn đối với những người uống ít hơn. Hiệu ứng điều chỉnh này không khác nhau giữa nam và nữ hoặc giữa các nhóm chủng tộc. Kết luận: Các chương trình phòng ngừa uống rượu ở trường đại học nên đảm bảo rằng sinh viên nhận thức được giới hạn của PBS như một biện pháp giảm thiểu các vấn đề về rượu."} +{"idx": 1870833, "en": "Infantile tremor syndrome (ITS) owing to vitamin B12 deficiency usually presents with tremors, anaemia, pigmentary skin changes, neuro-regression and hypotonia. A 10-month-old boy with ITS and respiratory failure owing to bilateral diaphragmatic palsy who responded to high parenteral doses of vitamin B12 is presented. As far as we are aware, this is the first report of diaphragmatic palsy associated with ITS and vitamin B12 deficiency.", "gemini": "Hội chứng run ở trẻ sơ sinh (ITS) do thiếu vitamin B12 thường biểu hiện bằng run, thiếu máu, thay đổi sắc tố da, thoái triển thần kinh và hạ huyết áp. Một bé trai 10 tháng tuổi mắc ITS và suy hô hấp do liệt cơ hoành hai bên đã đáp ứng với liều vitamin B12 tiêm tĩnh mạch cao. Theo như chúng tôi biết, đây là báo cáo đầu tiên về liệt cơ hoành liên quan đến ITS và thiếu vitamin B12."} +{"idx": 1870834, "en": "Commitment to leisure is recognized as an important aspect in understanding older adults' leisure behavior. Older adults' participation in Flow and Balance (FAB) is an exemplary instance of such engagement. We interviewed 11 older women who participate in FAB to explore how this experience contributed to coping with chronic illnesses. Our analysis revealed six central themes: (a) overcoming adversities; (b) investing personal effort; (c) stages of a leisure career; (d) health benefits and belongingness; (e) developing a special social world; and (f) centrality in life. Our findings emphasize the pivotal role of committed leisure in successful aging among older women.", "gemini": "Cam kết dành thời gian rảnh được công nhận là một khía cạnh quan trọng trong việc hiểu hành vi giải trí của người lớn tuổi. Sự tham gia của người lớn tuổi vào Flow and Balance (FAB) là một ví dụ điển hình về sự gắn kết như vậy. Chúng tôi đã phỏng vấn 11 phụ nữ lớn tuổi tham gia FAB để khám phá cách trải nghiệm này góp phần vào việc đối phó với các bệnh mãn tính. Phân tích của chúng tôi cho thấy sáu chủ đề chính: (a) vượt qua nghịch cảnh; (b) đầu tư nỗ lực cá nhân; (c) các giai đoạn của sự nghiệp giải trí; (d) lợi ích sức khỏe và tính thân thuộc; (e) phát triển một thế giới xã hội đặc biệt; và (f) trọng tâm trong cuộc sống. Những phát hiện của chúng tôi nhấn mạnh vai trò quan trọng của việc giải trí có cam kết trong quá trình lão hóa thành công ở những phụ nữ lớn tuổi."} +{"idx": 1870835, "en": "Hand, foot, and mouth disease (HFMD) is a common infectious gastrointestinal disease in children under the age of 5. Many studies have documented that meteorological variables, especially temperature, are associated with HFMD. Since the general climate patterns occur as latitude increases, so latitude may indirectly influence the peak time of HFMD. The objective of this study was to explore the effect of latitude on the starting of an HFMD epidemic in Dalian, which can help in the development of an early warning model of HFMD in difference latitude districts. Spearman's rank correlation coefficient was used to analyze the tendency of HFMD incidence rate over the year. A circular distribution method was used to calculate the gold standard of beginning of the HFMD epidemic. A negative binomial regression model was used to establish the early warning of the starting of the HFMD epidemic. The annualized crude incidence rate of HFMD disease in Dalian, Liaoning Province, China as a whole was 169.14 per 100,000 from 2009 to 2013.The incidence rate of HFMD varied considerably by district during the study period, but there was no significant declining or rising trend in disease incidence over the years by district of Dalian. The circular statistical analysis results showed that there was latitudinal gradient in the starting of the HFMD epidemic except for region B; the starting time of HFMD epidemic of Region A was earlier than other regions range 9 days to 18 days. The starting time of the HFMD epidemic differs from region to region with different latitudes in Dalian, Liaoning Province, China. This result can provide a scientific basis for early warning of HFMD.", "gemini": "Bệnh tay chân miệng (HFMD) là một bệnh truyền nhiễm đường tiêu hóa phổ biến ở trẻ em dưới 5 tuổi. Nhiều nghiên cứu đã ghi nhận rằng các biến số khí tượng, đặc biệt là nhiệt độ, có liên quan đến HFMD. Vì các kiểu khí hậu chung xảy ra khi vĩ độ tăng, nên vĩ độ có thể ảnh hưởng gián tiếp đến thời gian đỉnh điểm của HFMD. Mục tiêu của nghiên cứu này là khám phá tác động của vĩ độ đối với sự khởi đầu của dịch HFMD ở Đại Liên, có thể giúp phát triển mô hình cảnh báo sớm về HFMD ở các quận có vĩ độ khác nhau. Hệ số tương quan bậc Spearman được sử dụng để phân tích xu hướng tỷ lệ mắc HFMD trong năm. Phương pháp phân phối tròn được sử dụng để tính toán tiêu chuẩn vàng của sự khởi đầu của dịch HFMD. Một mô hình hồi quy nhị thức âm được sử dụng để thiết lập cảnh báo sớm về sự khởi đầu của dịch HFMD. Tỷ lệ mắc bệnh HFMD thô hàng năm ở Đại Liên, tỉnh Liêu Ninh, Trung Quốc nói chung là 169,14 trên 100.000 dân từ năm 2009 đến năm 2013. Tỷ lệ mắc HFMD thay đổi đáng kể theo quận trong thời gian nghiên cứu, nhưng không có xu hướng giảm hoặc tăng đáng kể về tỷ lệ mắc bệnh theo các quận của Đại Liên. Kết quả phân tích thống kê hình tròn cho thấy có sự chênh lệch theo vĩ độ trong sự khởi đầu của dịch HFMD ngoại trừ vùng B; thời điểm bắt đầu dịch HFMD của Vùng A sớm hơn các vùng khác từ 9 đến 18 ngày. Thời điểm bắt đầu dịch HFMD khác nhau giữa các vùng có vĩ độ khác nhau ở Đại Liên, tỉnh Liêu Ninh, Trung Quốc. Kết quả này có thể cung cấp cơ sở khoa học để cảnh báo sớm về HFMD."} +{"idx": 1870836, "en": "This article analyzes the perceptions of sexual affective diversity among teachers in Spanish high schools. Specifically, we address LGBTphobia, and teacher intervention and training through a survey study of 119 teachers. We administered a questionnaire to investigate these issues based on other studies. The data were analyzed on the basis of descriptive statistics. We attempted to answer three research questions: How do teachers analyze LGBTphobia in high schools? What is their experience and teaching intervention with regard to affective and sexual diversity? What perception exists in high schools about the need for training and education on affective and sexual diversity? The study concludes that high schools and their communities remain hostile places for LGBT students. Therefore, incorporating training into teaching practice and rethinking pedagogy from a queer perspective is fundamental to building an inclusive educational culture.", "gemini": "Bài viết này phân tích nhận thức về sự đa dạng tình cảm giới tính trong số các giáo viên ở các trường trung học Tây Ban Nha. Cụ thể, chúng tôi giải quyết vấn đề LGBTphobia và sự can thiệp và đào tạo của giáo viên thông qua một nghiên cứu khảo sát trên 119 giáo viên. Chúng tôi đã tiến hành một bảng câu hỏi để điều tra các vấn đề này dựa trên các nghiên cứu khác. Dữ liệu được phân tích trên cơ sở thống kê mô tả. Chúng tôi đã cố gắng trả lời ba câu hỏi nghiên cứu: Giáo viên phân tích LGBTphobia ở các trường trung học như thế nào? Kinh nghiệm và sự can thiệp giảng dạy của họ đối với sự đa dạng về tình cảm và giới tính là gì? Nhận thức nào tồn tại ở các trường trung học về nhu cầu đào tạo và giáo dục về sự đa dạng về tình cảm và giới tính? Nghiên cứu kết luận rằng các trường trung học và cộng đồng của họ vẫn là những nơi thù địch đối với học sinh LGBT. Do đó, việc đưa đào tạo vào thực hành giảng dạy và suy nghĩ lại về sư phạm từ góc độ kỳ quặc là điều cơ bản để xây dựng một nền văn hóa giáo dục toàn diện."} +{"idx": 1870837, "en": "OBJECTIVE The current state of portable/wearable electromyographic (EMG) devices for assessment of bruxism was reviewed. METHODS A search of full-text articles relevant to portable/wearable EMG devices capable of being used at home was performed. The data source used was MEDLINE via PubMed from January 1970 to July 2019. RESULTS There were nine kinds of wearable EMG devices capable of being used under unrestrained conditions. Ultra-miniaturized wearable EMG devices with a level of performance equivalent to that of conventional stationary EMG devices have been developed and are being used during sleep and in the daytime. The devices have a high level of diagnostic accuracy for sleep bruxism. A definite cut-off value for awake bruxism has not been established. DISCUSSION Assessment of sleep bruxism with a high level of accuracy can be performed using a portable/wearable EMG device. However, a definite cut-off value is required for assessment of awake bruxism.", "gemini": "MỤC TIÊU Đánh giá tình trạng hiện tại của các thiết bị đo điện cơ đồ (EMG) di động/có thể đeo được để đánh giá tình trạng nghiến răng. PHƯƠNG PHÁP Thực hiện tìm kiếm các bài báo toàn văn có liên quan đến các thiết bị EMG di động/có thể đeo được có khả năng sử dụng tại nhà. Nguồn dữ liệu sử dụng là MEDLINE qua PubMed từ tháng 1 năm 1970 đến tháng 7 năm 2019. KẾT QUẢ Có chín loại thiết bị EMG đeo được có khả năng sử dụng trong điều kiện không bị hạn chế. Các thiết bị EMG đeo được siêu nhỏ có mức hiệu suất tương đương với các thiết bị EMG cố định thông thường đã được phát triển và đang được sử dụng trong khi ngủ và ban ngày. Các thiết bị có độ chính xác chẩn đoán cao đối với tình trạng nghiến răng khi ngủ. Chưa thiết lập được giá trị ngưỡng chắc chắn cho tình trạng nghiến răng khi thức. THẢO LUẬN Có thể đánh giá tình trạng nghiến răng khi ngủ với độ chính xác cao bằng cách sử dụng thiết bị EMG di động/có thể đeo được. Tuy nhiên, cần có giá trị ngưỡng chắc chắn để đánh giá tình trạng nghiến răng khi thức."} +{"idx": 1870838, "en": "Context: Colorectal cancer (CRC) is the second leading cause of cancer related deaths in the US. There is paucity of data regarding CRC and the spinal cord injury (SCI) community. Persons with SCI have suboptimal rates of colonoscopies and face extensive barriers to care. The aim of our study was to compare CRC mortality in persons with SCI to CRC mortality in the general population.Design: A prospective follow-up study.Setting: Analysis of the National SCI database.Participants: 54,965 persons with SCI.Interventions: Not applicable.Outcome Measures: Current survival status and causes of death were determined. The expected number of CRC deaths was calculated for the general US population, using ICD-10 codes. Standardized mortality ratios (SMR) were calculated as the ratio of observed to expected CRC deaths stratified by current age, sex, race, time post-injury and neurologic group.Results: The CRC mortality was 146 persons out of 54,965 persons with SCI. The overall SMR was determined to be 1.11 (95% CI [0.94, 1.31]). Among subgroups, one finding was significant and this was for patients with injury level C1-4 with an American Spinal Injury Association Impairment Scale Grade of A, B or C with an SMR of 1.68 ([95% CI [1.03-2.61]).Conclusion: Although persons with SCI receive suboptimal rates of preventative care screenings and report extensive barriers to care, overall, they are not at an increased risk of CRC mortality. The current recommendations for CRC screening should be continued for these individuals while reducing barriers to care.", "gemini": "Bối cảnh: Ung thư trực tràng (CRC) là nguyên nhân gây tử vong do ung thư đứng thứ hai tại Hoa Kỳ. Dữ liệu về CRC và cộng đồng chấn thương tủy sống (SCI) còn rất ít. Những người bị SCI có tỷ lệ nội soi đại tràng không đạt mức tối ưu và phải đối mặt với nhiều rào cản trong việc chăm sóc. Mục đích của nghiên cứu của chúng tôi là so sánh tỷ lệ tử vong do CRC ở những người bị SCI với tỷ lệ tử vong do CRC ở dân số nói chung. Thiết kế: Một nghiên cứu theo dõi có triển vọng. Bối cảnh: Phân tích cơ sở dữ liệu SCI quốc gia. Người tham gia: 54.965 người bị SCI. Can thiệp: Không áp dụng. Các biện pháp đánh giá kết quả: Trạng thái sống sót hiện tại và nguyên nhân tử vong đã được xác định. Số ca tử vong do CRC dự kiến đã được tính toán cho dân số Hoa Kỳ nói chung, sử dụng mã ICD-10. Tỷ lệ tử vong chuẩn hóa (SMR) được tính là tỷ lệ giữa số ca tử vong do CRC quan sát được và số ca tử vong do CRC dự kiến được phân tầng theo độ tuổi hiện tại, giới tính, chủng tộc, thời gian sau chấn thương và nhóm thần kinh. Kết quả: Tỷ lệ tử vong do CRC là 146 người trong số 54.965 người bị SCI. SMR tổng thể được xác định là 1,11 (95% CI [0,94, 1,31]). Trong số các nhóm phụ, một phát hiện có ý nghĩa là đối với những bệnh nhân bị chấn thương mức độ C1-4 với Điểm thang đánh giá mức độ khuyết tật của Hiệp hội chấn thương tủy sống Hoa Kỳ là A, B hoặc C với SMR là 1,68 ([95% CI [1,03-2,61]). Kết luận: Mặc dù những người bị SCI có tỷ lệ sàng lọc chăm sóc phòng ngừa không đạt mức tối ưu và báo cáo nhiều rào cản trong việc chăm sóc, nhưng nhìn chung, họ không có nguy cơ tử vong do CRC cao hơn. Các khuyến nghị hiện hành về sàng lọc CRC nên được tiếp tục cho những cá nhân này trong khi giảm các rào cản trong việc chăm sóc."} +{"idx": 1870839, "en": "OBJECTIVE This study aimed to demonstrate the reproducibility of the Rocabado pain map (RPM) through its application by different evaluators. METHODS Forty participants (18-45 years old) were evaluated. The examiners applied RPM by searching for painful areas, and their intensities were measured through the visual analog scale (VAS). In addition, presence and degree of joint laxity were estimated. RESULTS Reproducibility values for antero-inferior synovial (zone 1), lateral ligament (zone 3), temporomandibular ligament (zone 4), posterior-inferior synovial (zone 5), and posterior ligament (zone 7) showed high intraclass correlation (ICC) values (above 0.7). Anterior-superior synovial (zone 2) and posterior-superior synovial (zone 6) showed ICC values above 0.4. DISCUSSION RPM is reproducible. A \"fair\" concordance according to Fleiss's criteria in zone 2 (ICC 0.42) and zone 6 (ICC 0.64) might be explained by the difficulties involved in moving the condyle from the upper synovial (zones 1,5) to the lower synovial (zones 2,6).", "gemini": "MỤC TIÊU Nghiên cứu này nhằm chứng minh khả năng tái tạo của bản đồ đau Rocabado (RPM) thông qua việc áp dụng của các chuyên gia đánh giá khác nhau. PHƯƠNG PHÁP Đã đánh giá 40 người tham gia (18-45 tuổi). Các bác sĩ đã áp dụng RPM bằng cách tìm kiếm các vùng đau và cường độ của chúng được đo thông qua thang đánh giá tương tự thị giác (VAS). Ngoài ra, sự hiện diện và mức độ lỏng lẻo của khớp được ước tính. KẾT QUẢ Các giá trị tái tạo cho màng hoạt dịch trước dưới (vùng 1), dây chằng bên (vùng 3), dây chằng thái dương hàm (vùng 4), màng hoạt dịch sau dưới (vùng 5) và dây chằng sau (vùng 7) cho thấy các giá trị tương quan nội nhóm (ICC) cao (trên 0,7). Màng hoạt dịch trước trên (vùng 2) và màng hoạt dịch sau trên (vùng 6) cho thấy các giá trị ICC trên 0,4. THẢO LUẬN RPM có khả năng tái tạo. Sự nhất quán \"tương đối\" theo tiêu chí của Fleiss ở vùng 2 (ICC 0,42) và vùng 6 (ICC 0,64) có thể được giải thích bằng những khó khăn liên quan đến việc di chuyển lồi cầu từ màng hoạt dịch trên (vùng 1,5) đến màng hoạt dịch dưới (vùng 2,6)."} +{"idx": 1870840, "en": "On the surface of endothelial cells (ECs) lies the glycocalyx, a barrier of polysaccharides that isolates the ECs from the blood. The role of the glycocalyx is dynamic and complex, thanks to not only its structure, but its vast number of components, one being hyaluronan (HA). HA is a critical component of the glycocalyx, having been found to have a wide variety of functions depending on its molecular weight, its modification, and receptor-ligand interactions. As HA and viscous blood are in constant contact, HA can transmit mechanosensory information directly to the cytoskeleton of the ECs. The degradation and synthesis of HA directly alters the permeability of the EC barrier; HA modulation not only alters the physical barrier but also can signal the initiation of other pathways. EC proliferation and angiogenesis are in part regulated by HA fragmentation, HA-dependent receptor binding, and downstream signals. The interaction between the CD44 receptor and HA is a driving force behind leukocyte recruitment, but each class of leukocyte still interacts with HA in unique ways during inflammation. HA regulates a diverse repertoire of EC functions.", "gemini": "Trên bề mặt của các tế bào nội mô (EC) nằm lớp glycocalyx, một hàng rào polysaccharides cô lập các EC khỏi máu. Vai trò của glycocalyx rất năng động và phức tạp, không chỉ nhờ vào cấu trúc của nó mà còn nhờ số lượng lớn các thành phần của nó, một trong số đó là hyaluronan (HA). HA là một thành phần quan trọng của glycocalyx, được phát hiện có nhiều chức năng khác nhau tùy thuộc vào trọng lượng phân tử, sự biến đổi và tương tác thụ thể-ligand của nó. Vì HA và máu nhớt tiếp xúc liên tục, HA có thể truyền thông tin cảm ứng cơ học trực tiếp đến bộ khung tế bào của các EC. Sự thoái hóa và tổng hợp HA làm thay đổi trực tiếp tính thấm của hàng rào EC; sự điều chỉnh HA không chỉ làm thay đổi hàng rào vật lý mà còn có thể báo hiệu sự khởi đầu của các con đường khác. Sự tăng sinh EC và sự hình thành mạch máu một phần được điều chỉnh bởi sự phân mảnh HA, sự liên kết thụ thể phụ thuộc HA và các tín hiệu hạ nguồn. Sự tương tác giữa thụ thể CD44 và HA là động lực thúc đẩy quá trình tuyển dụng bạch cầu, nhưng mỗi loại bạch cầu vẫn tương tác với HA theo những cách riêng biệt trong quá trình viêm. HA điều chỉnh một loạt các chức năng EC đa dạng."} +{"idx": 1870841, "en": "The LGBT community faces disproportionate healthcare disparities; poorly trained providers and stigma are likely contributors. Objectives: To evaluate the attitudes, opinions, and knowledge level of medical students regarding LGBT people and to examine which demographic variables are associated with more negative attitudes toward LGBT people. A cross-sectional study surveyed 561 medical students at Hanoi Medical University. Students held positive or very positive attitudes of both lesbians (96.3%) and gay men (88.1%). Students who were younger, female, and those with close relationships to LGBT people tended to have more positive attitudes toward gays and lesbians. The average score for the knowledge scale was 12.5 (total 32 questions) with a standard deviation of 4.7. Knowledge scores and positives attitudes were significantly, directly associated. Most students responded that there should be more LGBT-related content added in the curriculum. More research is needed regarding students in other health professions and at other institutions.", "gemini": "Cộng đồng LGBT phải đối mặt với sự chênh lệch chăm sóc sức khỏe không cân xứng; các nhà cung cấp được đào tạo kém và sự kỳ thị có thể là những yếu tố góp phần. Mục tiêu: Đánh giá thái độ, quan điểm và trình độ kiến thức của sinh viên y khoa liên quan đến người LGBT và xem xét các biến nhân khẩu học nào có liên quan đến thái độ tiêu cực hơn đối với người LGBT. Một nghiên cứu cắt ngang đã khảo sát 561 sinh viên y khoa tại Đại học Y Hà Nội. Sinh viên có thái độ tích cực hoặc rất tích cực đối với cả đồng tính nữ (96,3%) và nam đồng tính (88,1%). Những sinh viên trẻ tuổi hơn, nữ và những người có mối quan hệ gần gũi với người LGBT có xu hướng có thái độ tích cực hơn đối với người đồng tính nam và đồng tính nữ. Điểm trung bình cho thang điểm kiến thức là 12,5 (tổng 32 câu hỏi) với độ lệch chuẩn là 4,7. Điểm kiến thức và thái độ tích cực có liên quan trực tiếp và đáng kể. Hầu hết sinh viên trả lời rằng cần phải thêm nhiều nội dung liên quan đến LGBT vào chương trình giảng dạy. Cần nhiều nghiên cứu hơn về sinh viên ở các ngành nghề y tế khác và tại các cơ sở khác."} +{"idx": 1870842, "en": "OBJECTIVE To review the knowledge, attitude, and clinical practice of dentists and dental specialists toward obstructive sleep apnea (OSA). METHODS A literature search was performed on PubMed and ScienceDirect databases. Peer-reviewed English publications were reviewed. No time limit was applied. RESULTS Four articles were included for the review. Years in the profession, specialist training, and gender were associated with the level of knowledge about OSA. Weight control was the most recognizable treatment for patients with OSA. Dentists agreed that OSA is a life-threatening disease, and they could be involved in detecting and treating OSA. Most dentists had no clinical experience of managing patients with OSA. CONCLUSION Dentists had a certain level of knowledge about OSA and were willing to deal with OSA. However, they lacked clinical experience. They were also enthusiastic about further education on OSA.", "gemini": "MỤC TIÊU Xem xét kiến thức, thái độ và thực hành lâm sàng của nha sĩ và bác sĩ chuyên khoa răng hàm mặt đối với chứng ngưng thở khi ngủ tắc nghẽn (OSA). PHƯƠNG PHÁP Tìm kiếm tài liệu đã được thực hiện trên các cơ sở dữ liệu PubMed và ScienceDirect. Các ấn phẩm tiếng Anh được bình duyệt đã được xem xét. Không áp dụng giới hạn thời gian. KẾT QUẢ Có bốn bài báo được đưa vào để xem xét. Số năm trong nghề, đào tạo chuyên khoa và giới tính có liên quan đến mức độ hiểu biết về OSA. Kiểm soát cân nặng là phương pháp điều trị dễ nhận biết nhất đối với bệnh nhân mắc OSA. Các nha sĩ đồng ý rằng OSA là một căn bệnh đe dọa tính mạng và họ có thể tham gia phát hiện và điều trị OSA. Hầu hết các nha sĩ không có kinh nghiệm lâm sàng trong việc quản lý bệnh nhân mắc OSA. KẾT LUẬN Các nha sĩ có một số kiến thức nhất định về OSA và sẵn sàng đối phó với OSA. Tuy nhiên, họ thiếu kinh nghiệm lâm sàng. Họ cũng rất nhiệt tình với việc giáo dục thêm về OSA."} +{"idx": 1870843, "en": "OBJECTIVE To use the Italian version of the General Sleep Disturbance Scale (GSDS-IT) to assess the psychometric properties in a sample of orthopedic patients. METHODS Participants were divided into sleepless and non-sleepless according to the Diagnostic and Statistical Manual of Mental Disorders Fifth Edition (DSM-V). Reliability was assessed with Cronbach's alpha. To evaluate responsiveness, the scale was administered a second time after the rehabilitation program. RESULTS The scale was administered to the 158 patients recruited. The GSDS-IT had good internal consistency (Cronbach's α of 0.752) and good responsiveness (ICC of 0.78). The optimal cut-off point for the GSDS-IT was 48.5. CONCLUSION The GSDS-IT was demonstrated to be a valid and rapid tool to detect the presence of sleep disorders in a clinical setting in patients admitted to an intensive care unit and allowed the detection of the onset one week after admission and persisting until the time of discharge.", "gemini": "MỤC TIÊU Sử dụng phiên bản tiếng Ý của Thang Đánh giá Rối loạn Giấc ngủ Chung (GSDS-IT) để đánh giá các đặc tính tâm lý trong mẫu bệnh nhân chỉnh hình. PHƯƠNG PHÁP Những người tham gia được chia thành mất ngủ và không mất ngủ theo Sổ tay Chẩn đoán và Thống kê Rối loạn Tâm thần Phiên bản thứ năm (DSM-V). Độ tin cậy được đánh giá bằng hệ số alpha của Cronbach. Để đánh giá khả năng đáp ứng, thang đo đã được thực hiện lần thứ hai sau chương trình phục hồi chức năng. KẾT QUẢ Thang đo đã được thực hiện cho 158 bệnh nhân được tuyển dụng. GSDS-IT có độ tin cậy nội bộ tốt (alpha Cronbach là 0,752) và khả năng đáp ứng tốt (ICC là 0,78). Điểm cắt tối ưu cho GSDS-IT là 48,5. KẾT LUẬN GSDS-IT đã được chứng minh là một công cụ hợp lệ và nhanh chóng để phát hiện sự hiện diện của các rối loạn giấc ngủ trong môi trường lâm sàng ở những bệnh nhân được đưa vào đơn vị chăm sóc đặc biệt và cho phép phát hiện sự khởi phát một tuần sau khi nhập viện và kéo dài cho đến thời điểm xuất viện."} +{"idx": 1870844, "en": "The duodenum, the first portion of the small bowel, is divided into four segments and extends to both retro- and intraperitoneal spaces. Some conditions arise primarily from the duodenum, but it can be secondarily affected by processes that involve neighboring structures. When duodenal emergencies are not identified and treated promptly, they may result in high morbidity and mortality. Imaging plays an important role in the diagnosis of duodenal conditions in the acute setting. However, the radiologic findings can be subtle, and awareness of relevant patient history and clinical presentation is important as it may increase the index of suspicion and one's ability to diagnose these conditions. Duodenal peptic disease is common and can be complicated by bleeding and perforation. The duodenum can be secondarily involved by pancreatitis and gallbladder pathologic conditions and may be affected by iatrogenic complications following endoscopic procedures. Traumatic injuries to the duodenum are generally uncommon, with penetrating traumatic injury being the most frequent mechanism of injury. Duodenal vascular pathologic conditions such as aortoduodenal fistula are uncommon but can be life threatening. The knowledge of which pathologic condition can involve which duodenal segment can help the radiologist establish a differential diagnosis and achieve a more targeted imaging approach. The online slide presentation from the RSNA Annual Meeting is available for this article. ©RSNA, 2020.", "gemini": "Tà tràng, phần đầu tiên của ruột non, được chia thành bốn đoạn và kéo dài đến cả khoang phúc mạc và khoang sau phúc mạc. Một số tình trạng chủ yếu phát sinh từ tá tràng, nhưng nó có thể bị ảnh hưởng thứ phát bởi các quá trình liên quan đến các cấu trúc lân cận. Khi các trường hợp cấp cứu tá tràng không được xác định và điều trị kịp thời, chúng có thể dẫn đến tỷ lệ mắc bệnh và tử vong cao. Chẩn đoán hình ảnh đóng một vai trò quan trọng trong chẩn đoán các tình trạng tá tràng trong trường hợp cấp tính. Tuy nhiên, các phát hiện trên X quang có thể rất tinh tế và việc nhận thức được tiền sử bệnh nhân có liên quan và biểu hiện lâm sàng là rất quan trọng vì nó có thể làm tăng chỉ số nghi ngờ và khả năng chẩn đoán các tình trạng này. Bệnh loét tá tràng do pepsin là phổ biến và có thể phức tạp do chảy máu và thủng. Tà tràng có thể bị ảnh hưởng thứ phát bởi viêm tụy và các tình trạng bệnh lý của túi mật và có thể bị ảnh hưởng bởi các biến chứng do thầy thuốc gây ra sau các thủ thuật nội soi. Chấn thương do chấn thương ở tá tràng thường không phổ biến, trong đó chấn thương xuyên thấu là cơ chế chấn thương thường gặp nhất. Các tình trạng bệnh lý mạch máu tá tràng như lỗ rò động mạch chủ tá tràng không phổ biến nhưng có thể đe dọa tính mạng. Việc biết được tình trạng bệnh lý nào có thể liên quan đến đoạn tá tràng nào có thể giúp bác sĩ chẩn đoán hình ảnh thiết lập chẩn đoán phân biệt và đạt được phương pháp tiếp cận chẩn đoán hình ảnh có mục tiêu hơn. Bài thuyết trình trực tuyến từ Hội nghị thường niên của RSNA có sẵn cho bài viết này. © RSNA, 2020."} +{"idx": 1870845, "en": "Immunoglobulin G4-related disease (IgG4-RD) is a systemic fibroinflammatory disease characterized by focal or diffuse organ infiltration of IgG4-bearing plasma cells. The diagnosis of IgG4-RD is based on a combination of clinical, serologic, radiologic, and histopathologic findings. IgG4-RD has been reported to affect almost all organ systems. The kidney is the most frequently involved of the genitourinary organs. The most common renal manifestation of IgG4-RD is IgG4-RD tubulointerstitial nephritis, followed by membranous glomerulonephropathy and, less frequently, obstructive nephropathy involving the renal pelvis, ureter, or retroperitoneum. Renal parenchymal lesions may appear as multiple nodular lesions, diffuse patchy infiltrative lesions, or a single nodular lesion. Multiple small nodular cortical lesions are the most common imaging findings of IgG4-RD involving the kidney. Renal pelvic, sinus, or perinephric lesions can also occur. IgG4-RD involvement of other genitourinary organs including the ureter, bladder, urethra, and male and female reproductive organs is rare compared with kidney involvement but may show variable imaging findings such as a localized mass within or surrounding the involved organ or diffuse enlargement of the involved organ. Imaging findings of IgG4-RD involving the genitourinary system are nonspecific but should be differentiated from inflammatory and neoplastic lesions that mimic IgG4-RD. The online slide presentation from the RSNA Annual Meeting is available for this article. ©RSNA, 2020.", "gemini": "Bệnh liên quan đến Immunoglobulin G4 (IgG4-RD) là một bệnh viêm xơ hệ thống đặc trưng bởi sự thâm nhiễm của tế bào plasma mang IgG4 ở các cơ quan khu trú hoặc lan tỏa. Chẩn đoán IgG4-RD dựa trên sự kết hợp của các phát hiện lâm sàng, huyết thanh học, X quang và mô bệnh học. IgG4-RD đã được báo cáo là ảnh hưởng đến hầu hết các hệ cơ quan. Thận là cơ quan thường bị ảnh hưởng nhất trong số các cơ quan sinh dục tiết niệu. Biểu hiện thận phổ biến nhất của IgG4-RD là viêm thận kẽ IgG4-RD, tiếp theo là viêm cầu thận màng và ít gặp hơn là bệnh thận tắc nghẽn liên quan đến bể thận, niệu quản hoặc phúc mạc sau. Các tổn thương nhu mô thận có thể xuất hiện dưới dạng nhiều tổn thương dạng nốt, tổn thương thâm nhiễm từng mảng lan tỏa hoặc tổn thương dạng nốt đơn lẻ. Nhiều tổn thương vỏ não dạng nốt nhỏ là những phát hiện hình ảnh phổ biến nhất của IgG4-RD liên quan đến thận. Các tổn thương ở bể thận, xoang thận hoặc quanh thận cũng có thể xảy ra. IgG4-RD liên quan đến các cơ quan sinh dục tiết niệu khác bao gồm niệu quản, bàng quang, niệu đạo và cơ quan sinh sản của nam và nữ rất hiếm so với tình trạng liên quan đến thận nhưng có thể biểu hiện các phát hiện hình ảnh khác nhau như khối u khu trú bên trong hoặc xung quanh cơ quan bị ảnh hưởng hoặc phì đại lan tỏa của cơ quan bị ảnh hưởng. Các phát hiện hình ảnh của IgG4-RD liên quan đến hệ thống sinh dục tiết niệu không đặc hiệu nhưng cần phân biệt với các tổn thương viêm và tân sinh bắt chước IgG4-RD. Bài thuyết trình trực tuyến từ Hội nghị thường niên của RSNA có sẵn cho bài viết này. © RSNA, 2020."} +{"idx": 1870846, "en": "The recent shutting down of in-person events owing to the coronavirus disease 2019 (COVID-19) pandemic has elevated the popularity of web-based conferencing. This development provides an opportunity for educators to test their teaching skills on what, for many, is a new platform. Many of the basic elements of what constitutes an effective presentation are the same regardless of whether they are delivered in person or online. However, there are advantages and disadvantages of each mode of presentation, and understanding how to best leverage the features of an online platform will lead to a better educational experience for the presenter and audience. The effectiveness of any presentation is dependent on the ability of the speaker to communicate with the audience. This is accomplished by including as much audience participation as possible. Many of the techniques used to encourage audience participation in person can be adapted for use in online presentations (eg, the use of features such as chat, hand raising, polling, and question-and-answer sessions). In any type of presentation, both the quality of the content and the oral delivery are important. The author reviews the common elements of an effective presentation and how they can be optimized for online platforms. ©RSNA, 2020.", "gemini": "Việc đóng cửa các sự kiện trực tiếp gần đây do đại dịch bệnh do vi-rút corona 2019 (COVID-19) đã làm gia tăng sự phổ biến của hội nghị trên nền tảng web. Sự phát triển này mang đến cơ hội cho các nhà giáo dục kiểm tra các kỹ năng giảng dạy của mình trên nền tảng mới đối với nhiều người. Nhiều yếu tố cơ bản cấu thành nên một bài thuyết trình hiệu quả là như nhau bất kể chúng được truyền đạt trực tiếp hay trực tuyến. Tuy nhiên, mỗi phương thức thuyết trình đều có những ưu và nhược điểm và hiểu cách tận dụng tốt nhất các tính năng của nền tảng trực tuyến sẽ mang lại trải nghiệm giáo dục tốt hơn cho người thuyết trình và khán giả. Hiệu quả của bất kỳ bài thuyết trình nào đều phụ thuộc vào khả năng giao tiếp với khán giả của người nói. Điều này đạt được bằng cách bao gồm càng nhiều sự tham gia của khán giả càng tốt. Nhiều kỹ thuật được sử dụng để khuyến khích sự tham gia của khán giả trực tiếp có thể được điều chỉnh để sử dụng trong các bài thuyết trình trực tuyến (ví dụ: sử dụng các tính năng như trò chuyện, giơ tay, thăm dò ý kiến và các phiên hỏi đáp). Trong bất kỳ loại bài thuyết trình nào, cả chất lượng nội dung và cách truyền đạt bằng lời đều rất quan trọng. Tác giả xem xét các yếu tố chung của một bài thuyết trình hiệu quả và cách tối ưu hóa chúng cho các nền tảng trực tuyến. ©RSNA, 2020."} +{"idx": 1870847, "en": "Significance: Electric factors such as electric charges, electrodynamic field, skin battery, and interstitial exclusion permeate wound healing physiology and physiopathology from injury to re-epithelialization. The understanding of how electric factors contribute to wound healing and how treatments may interfere with them is fundamental for the development of better strategies for the management of pathological scarring and chronic wounds. Recent Advances: Angiogenesis, cell migration, macrophage activation hemorheology, and microcirculation can interfere and be interfered with electric factors. New treatments with various types of electric currents, laser, light emitting diode, acupuncture, and weak electric fields applied directly on the wound have been developed to improve wound healing. Critical Issues: Despite the basic and clinical development, pathological scars such as keloids and chronic wounds are still a challenge. Future Directions: New treatments can be developed to improve skin wound healing taking into account the influence of electrical charges. Monitoring electrical activity during skin healing and the influence of treatments on hemorheology and microcirculation are examples of how to use knowledge of electrical factors to increase their effectiveness.", "gemini": "Ý nghĩa: Các yếu tố điện như điện tích, trường điện động, pin da và sự loại trừ kẽ mô thấm vào quá trình sinh lý và bệnh lý học lành vết thương từ khi bị thương đến khi biểu mô lại. Việc hiểu cách các yếu tố điện góp phần vào quá trình lành vết thương và cách các phương pháp điều trị có thể ảnh hưởng đến chúng là rất cơ bản đối với việc phát triển các chiến lược tốt hơn để quản lý sẹo bệnh lý và vết thương mạn tính. Những tiến bộ gần đây: Sinh mạch, di chuyển tế bào, hoạt hóa đại thực bào, huyết học và vi tuần hoàn có thể can thiệp và bị can thiệp bởi các yếu tố điện. Các phương pháp điều trị mới với các loại dòng điện, tia laser, điốt phát sáng, châm cứu và trường điện yếu khác nhau được áp dụng trực tiếp lên vết thương đã được phát triển để cải thiện quá trình lành vết thương. Các vấn đề quan trọng: Mặc dù có sự phát triển cơ bản và lâm sàng, các vết sẹo bệnh lý như sẹo lồi và vết thương mạn tính vẫn là một thách thức. Các hướng phát triển trong tương lai: Các phương pháp điều trị mới có thể được phát triển để cải thiện quá trình lành vết thương trên da, có tính đến ảnh hưởng của điện tích. Theo dõi hoạt động điện trong quá trình lành vết thương trên da và ảnh hưởng của các phương pháp điều trị đối với huyết học và vi tuần hoàn là những ví dụ về cách sử dụng kiến thức về các yếu tố điện để tăng hiệu quả của chúng."} +{"idx": 1870848, "en": "Objective: High bacterial load contributes to chronicity of wounds and is diagnosed based on assessment of clinical signs and symptoms (CSS) of infection, but these characteristics are poor predictors of bacterial burden. Point-of-care fluorescence imaging (FL) MolecuLight i:X can improve identification of wounds with high bacterial burden (>104 colony-forming unit [CFU]/g). FL detects bacteria, whether planktonic or in biofilm, but does not distinguish between the two. In this study, diagnostic accuracy of FL was compared to CSS during routine wound assessment. Postassessment, clinicians were surveyed to assess impact of FL on treatment plan. Approach: A prospective multicenter controlled study was conducted by 20 study clinicians from 14 outpatient advanced wound care centers across the United States. Wounds underwent assessment for CSS followed by FL. Biopsies were collected to confirm total bacterial load. Three hundred fifty patients completed the study (138 diabetic foot ulcers, 106 venous leg ulcers, 60 surgical sites, 22 pressure ulcers, and 24 others). Results: Around 287/350 wounds (82%) had bacterial loads >104 CFU/g, and CSS missed detection of 85% of these wounds. FL significantly increased detection of bacteria (>104 CFU/g) by fourfold, and this was consistent across wound types (p < 0.001). Specificity of CSS+FL remained comparably high to CSS (p = 1.0). FL information modified treatment plans (69% of wounds), influenced wound bed preparation (85%), and improved overall patient care (90%) as reported by study clinicians. Innovation: This novel noncontact, handheld FL device provides immediate, objective information on presence, location, and load of bacteria at point of care. Conclusion: Use of FL facilitates adherence to clinical guidelines recommending prompt detection and removal of bacterial burden to reduce wound infection and facilitate healing.", "gemini": "Mục tiêu: Tải lượng vi khuẩn cao góp phần vào tình trạng mạn tính của vết thương và được chẩn đoán dựa trên đánh giá các dấu hiệu và triệu chứng lâm sàng (CSS) của nhiễm trùng, nhưng những đặc điểm này là yếu tố dự báo kém về gánh nặng vi khuẩn. Chẩn đoán hình ảnh huỳnh quang tại điểm chăm sóc (FL) MolecuLight i:X có thể cải thiện khả năng xác định các vết thương có tải lượng vi khuẩn cao (>104 đơn vị hình thành khuẩn lạc [CFU]/g). FL phát hiện vi khuẩn, bất kể ở dạng sinh vật phù du hay trong màng sinh học, nhưng không phân biệt giữa hai loại này. Trong nghiên cứu này, độ chính xác chẩn đoán của FL đã được so sánh với CSS trong quá trình đánh giá vết thương thông thường. Sau khi đánh giá, các bác sĩ lâm sàng được khảo sát để đánh giá tác động của FL đến kế hoạch điều trị. Tiếp cận: Một nghiên cứu đối chứng đa trung tâm có triển vọng đã được thực hiện bởi 20 bác sĩ lâm sàng nghiên cứu từ 14 trung tâm chăm sóc vết thương tiên tiến ngoại trú trên khắp Hoa Kỳ. Các vết thương đã được đánh giá CSS sau đó là FL. Các mẫu sinh thiết được thu thập để xác nhận tổng lượng vi khuẩn. Ba trăm năm mươi bệnh nhân đã hoàn thành nghiên cứu (138 loét bàn chân do tiểu đường, 106 loét chân tĩnh mạch, 60 vị trí phẫu thuật, 22 loét tì đè và 24 trường hợp khác). Kết quả: Khoảng 287/350 vết thương (82%) có tải lượng vi khuẩn> 104 CFU/g và CSS bỏ sót 85% các vết thương này. FL làm tăng đáng kể việc phát hiện vi khuẩn (> 104 CFU/g) gấp bốn lần và điều này phù hợp trên tất cả các loại vết thương (p <0,001). Độ đặc hiệu của CSS+FL vẫn cao tương đương với CSS (p = 1,0). Thông tin FL đã sửa đổi các kế hoạch điều trị (69% vết thương), ảnh hưởng đến việc chuẩn bị ổ vết thương (85%) và cải thiện tổng thể việc chăm sóc bệnh nhân (90%) theo báo cáo của các bác sĩ lâm sàng nghiên cứu. Đổi mới: Thiết bị FL cầm tay, không tiếp xúc mới lạ này cung cấp thông tin khách quan, tức thời về sự hiện diện, vị trí và tải lượng vi khuẩn tại điểm chăm sóc. Kết luận: Việc sử dụng FL tạo điều kiện thuận lợi cho việc tuân thủ các hướng dẫn lâm sàng khuyến nghị phát hiện và loại bỏ nhanh chóng gánh nặng vi khuẩn để giảm nhiễm trùng vết thương và tạo điều kiện thuận lợi cho quá trình lành bệnh."} +{"idx": 1870849, "en": "Patient safety events occur in health care, and root cause analysis (RCA) meetings held after these incidents often reveal valuable insights into systemic barriers between optimal processes or stated policies and actual practice, providing critical opportunities for improvement. The patient safety team that facilitates RCA meetings in the radiology department at the authors' institution received feedback suggesting dissatisfaction with the RCA process. The team followed a structured process improvement framework to analyze the root causes of this dissatisfaction and create a better system. Using a post-RCA survey to target satisfaction scores as an improvement goal, the team successfully increased participant and facilitator satisfaction levels with sustained results. The patient safety team applied structured process improvement methodologies to their own daily work, learning lessons about measuring difficult processes and choosing appropriate metrics, the benefits of standardized work, and how to continuously improve a quality program. In the course of improving the satisfaction of employees participating in the RCA process, a more robust, continuously improving patient safety program has emerged to enhance the ability of those within the department to report, learn from, and hopefully prevent patient safety events in the future.©RSNA, 2020.", "gemini": "Các sự cố an toàn bệnh nhân xảy ra trong chăm sóc sức khỏe và các cuộc họp phân tích nguyên nhân gốc rễ (RCA) được tổ chức sau những sự cố này thường tiết lộ những hiểu biết có giá trị về các rào cản hệ thống giữa các quy trình tối ưu hoặc các chính sách đã nêu và thực hành thực tế, mang lại cơ hội quan trọng để cải thiện. Nhóm an toàn bệnh nhân tạo điều kiện cho các cuộc họp RCA trong khoa X quang tại cơ sở của các tác giả đã nhận được phản hồi cho thấy sự không hài lòng với quy trình RCA. Nhóm đã tuân theo một khuôn khổ cải tiến quy trình có cấu trúc để phân tích các nguyên nhân gốc rễ của sự không hài lòng này và tạo ra một hệ thống tốt hơn. Sử dụng một cuộc khảo sát sau RCA để nhắm mục tiêu vào điểm số hài lòng như một mục tiêu cải tiến, nhóm đã thành công trong việc tăng mức độ hài lòng của người tham gia và người tạo điều kiện với kết quả bền vững. Nhóm an toàn bệnh nhân đã áp dụng các phương pháp cải tiến quy trình có cấu trúc vào công việc hàng ngày của chính họ, rút ra bài học về việc đo lường các quy trình khó khăn và lựa chọn các số liệu thích hợp, lợi ích của công việc được chuẩn hóa và cách liên tục cải thiện chương trình chất lượng. Trong quá trình cải thiện sự hài lòng của nhân viên tham gia vào quy trình RCA, một chương trình an toàn bệnh nhân mạnh mẽ hơn, liên tục cải thiện đã xuất hiện để nâng cao khả năng của những người trong khoa trong việc báo cáo, học hỏi từ và hy vọng ngăn ngừa các sự kiện an toàn bệnh nhân trong tương lai. © RSNA, 2020."} +{"idx": 1870850, "en": "Significance: Wound dressings are frequently used for wound covering and healing. Ideal wound dressings should provide a moist environment for wounds and actively promote wound healing and skin recovery. The materials used as ideal wound dressings should possess specific properties, thus accelerating skin tissue regeneration process. Recent Advances: Bacterial cellulose (BC) is a natural polymer synthesized by some bacteria. As a kind of natural biopolymer, BC shows good biological activity, biodegradability, and biological adaptability. It has many unique physical, chemical, and biological properties, such as ultrafine nanofiber network, high crystallinity, high water absorption and retention capacity, and high tensile strength and elastic modulus. These excellent properties of BC have laid the foundation for its application as dressing in wound healing. Critical Issues: To optimize the biocompatibility and antimicrobial activity of BC, different methods including microbial fermentation, physical modification, chemical modification, and compound modification have been adopted to modify BC to ensure a better application in wound healing. BC-based wound dressings have been applied in infected wounds, acute traumatic injuries, burns, and diabetic wounds, showing remarkable therapeutic effects on promoting wound healing. Furthermore, there have been some commercial BC-based dressings and they have been utilized in clinical practice. Future Directions: Because of its excellent physicochemical characteristics and biological properties, BC shows high clinical value to be used as a wound dressing for skin tissue regeneration.", "gemini": "Ý nghĩa: Băng vết thương thường được sử dụng để che và chữa lành vết thương. Băng vết thương lý tưởng nên cung cấp một môi trường ẩm cho vết thương và tích cực thúc đẩy quá trình lành vết thương và phục hồi da. Các vật liệu được sử dụng làm băng vết thương lý tưởng phải có các đặc tính cụ thể, do đó đẩy nhanh quá trình tái tạo mô da. Những tiến bộ gần đây: Cellulose vi khuẩn (BC) là một loại polyme tự nhiên được tổng hợp bởi một số loại vi khuẩn. Là một loại biopolymer tự nhiên, BC cho thấy hoạt tính sinh học, khả năng phân hủy sinh học và khả năng thích ứng sinh học tốt. Nó có nhiều tính chất vật lý, hóa học và sinh học độc đáo, chẳng hạn như mạng lưới nano siêu mịn, độ kết tinh cao, khả năng hấp thụ và giữ nước cao, độ bền kéo cao và mô đun đàn hồi. Những tính chất tuyệt vời này của BC đã đặt nền tảng cho ứng dụng của nó như một loại băng trong quá trình chữa lành vết thương. Các vấn đề quan trọng: Để tối ưu hóa khả năng tương thích sinh học và hoạt tính kháng khuẩn của BC, các phương pháp khác nhau bao gồm lên men vi sinh, sửa đổi vật lý, sửa đổi hóa học và sửa đổi hợp chất đã được áp dụng để sửa đổi BC để đảm bảo ứng dụng tốt hơn trong việc chữa lành vết thương. Băng vết thương gốc BC đã được ứng dụng trong các vết thương nhiễm trùng, chấn thương cấp tính, bỏng và vết thương do tiểu đường, cho thấy hiệu quả điều trị đáng kể trong việc thúc đẩy quá trình lành vết thương. Hơn nữa, đã có một số loại băng gốc BC thương mại và chúng đã được sử dụng trong thực hành lâm sàng. Hướng đi trong tương lai: Do có các đặc tính lý hóa và sinh học tuyệt vời, BC cho thấy giá trị lâm sàng cao khi được sử dụng làm băng vết thương để tái tạo mô da."} +{"idx": 1870851, "en": "Significance: Negative pressure wound therapy (NPWT) has become a valuable adjunct in the treatment of acute and chronic wounds in several surgical disciplines. Retained foams are among its side effects, and they pose a rare but devastating complication at the site of this therapy, which might be associated with wound-healing complications, infection persistence, repeated surgical revisions, and prolonged antibiotic courses. Recent Advances: In the past 15 years, an increasing number of studies have identified this potential problem. Although the exact incidence remains unknown, initial studies have indicated rates of up to 10% in large collectives being treated by NPWT. Critical Issues: The lack of radiopaque markers does not allow for the visual control of retained foams using plain radiographs. Further imaging methods (e.g., CT and MRI) also do not help in adequate differential diagnosis. The lack of routine documentation of the number, type, and localization of the inserted foam(s) and unplanned surgery with a different surgical team pose risk factors for foam retainment. Future Directions: Introducing new documentation records for wounds treated with NPWT is recommended. At foam removal, all dressing materials should be examined for integrity. The development of foams with radiopaque markers by the industry, such as those routinely used in surgical gauze swabs, might also be a useful step to minimize the risk of foam retainment within wounds.", "gemini": "Ý nghĩa: Liệu pháp áp lực âm (NPWT) đã trở thành một phương pháp hỗ trợ có giá trị trong điều trị các vết thương cấp tính và mãn tính trong một số chuyên khoa phẫu thuật. Bọt giữ lại là một trong những tác dụng phụ của nó, và chúng gây ra một biến chứng hiếm gặp nhưng tàn phá tại vị trí điều trị này, có thể liên quan đến các biến chứng lành vết thương, tình trạng nhiễm trùng dai dẳng, phẫu thuật chỉnh sửa nhiều lần và các đợt kháng sinh kéo dài. Những tiến bộ gần đây: Trong 15 năm qua, ngày càng có nhiều nghiên cứu xác định được vấn đề tiềm ẩn này. Mặc dù tỷ lệ mắc chính xác vẫn chưa được biết, các nghiên cứu ban đầu đã chỉ ra tỷ lệ lên tới 10% trong các nhóm lớn được điều trị bằng NPWT. Các vấn đề quan trọng: Việc thiếu các dấu hiệu cản quang không cho phép kiểm soát trực quan các bọt giữ lại bằng chụp X quang thông thường. Các phương pháp chụp ảnh khác (ví dụ: CT và MRI) cũng không giúp ích trong việc chẩn đoán phân biệt đầy đủ. Việc thiếu tài liệu thường quy về số lượng, loại và vị trí của bọt đã đưa vào và phẫu thuật không theo kế hoạch với một nhóm phẫu thuật khác gây ra các yếu tố rủi ro đối với việc giữ lại bọt. Hướng đi trong tương lai: Khuyến cáo đưa vào hồ sơ tài liệu mới để ghi lại các vết thương được điều trị bằng NPWT. Khi tháo bọt, tất cả các vật liệu băng nên được kiểm tra tính toàn vẹn. Việc phát triển các loại bọt có dấu hiệu cản quang bởi ngành công nghiệp, chẳng hạn như những loại thường được sử dụng trong gạc phẫu thuật, cũng có thể là một bước hữu ích để giảm thiểu nguy cơ giữ lại bọt trong vết thương."} +{"idx": 1870852, "en": "Significance: A moist wound environment has several benefits that result in faster and better quality of healing. It facilitates autolytic debridement, reduces pain, reduces scarring, activates collagen synthesis, facilitates and promotes keratinocyte migration over the wound surface, and supports the presence and function of nutrients, growth factors, and other soluble mediators in the wound microenvironment. Recent Advances: Wound dressings can be utilized to create, maintain, and control a moist environment for healing. Moist wound dressings can be divided into films, foams, hydrocolloids, hydrogels, and alginates. We are also including negative pressure wound therapy systems in the moist dressings. Critical Issues: An optimal wound dressing should provide a moist environment and have an optimal water vapor transmission rate (WVTR) and absorptive capacity. It should also protect the wound against trauma and contamination and be easy to apply, painless to remove, and esthetically acceptable or even pleasing. Future Directions: Interventions, particularly dressing changes, by medical caregivers are labor intensive and expensive and there should be a continuous effort to reduce their number per week. Smart dressings with integrated microsensors and delivery capabilities that would allow wireless real-time monitoring and treatment of the wound would be very advantageous. This way the state of the wound as well as the wear time of the dressing could be assessed without dressing removal or visit to the wound care center. In addition, an ability to adjust the WVTRs to the exudate level of the wound (or having a large absorptive capacity without changing the WVTR) would be useful. This feature would guarantee an optimal level of hydration of the wound surface throughout the treatment.", "gemini": "Ý nghĩa: Môi trường vết thương ẩm ướt có một số lợi ích giúp vết thương lành nhanh hơn và chất lượng tốt hơn. Nó tạo điều kiện cho quá trình tự phân hủy, giảm đau, giảm sẹo, kích hoạt tổng hợp collagen, tạo điều kiện và thúc đẩy quá trình di chuyển của tế bào sừng trên bề mặt vết thương và hỗ trợ sự hiện diện và chức năng của các chất dinh dưỡng, yếu tố tăng trưởng và các chất trung gian hòa tan khác trong môi trường vi mô của vết thương. Những tiến bộ gần đây: Băng vết thương có thể được sử dụng để tạo ra, duy trì và kiểm soát môi trường ẩm ướt để chữa lành. Băng vết thương ẩm có thể được chia thành màng, bọt, hydrocolloid, hydrogel và alginate. Chúng tôi cũng bao gồm các hệ thống trị liệu vết thương áp lực âm trong băng vết thương ẩm. Các vấn đề quan trọng: Một băng vết thương tối ưu nên tạo ra môi trường ẩm và có tốc độ truyền hơi nước (WVTR) và khả năng hấp thụ tối ưu. Nó cũng nên bảo vệ vết thương khỏi chấn thương và nhiễm trùng, dễ sử dụng, không đau khi tháo ra và có thể chấp nhận được về mặt thẩm mỹ hoặc thậm chí là đẹp. Hướng đi trong tương lai: Các biện pháp can thiệp, đặc biệt là thay băng, của các nhân viên y tế đòi hỏi nhiều nhân công và tốn kém, vì vậy cần phải có nỗ lực liên tục để giảm số lượng thay băng mỗi tuần. Băng thông minh tích hợp cảm biến siêu nhỏ và khả năng phân phối cho phép theo dõi và điều trị vết thương không dây theo thời gian thực sẽ rất có lợi. Bằng cách này, có thể đánh giá tình trạng vết thương cũng như thời gian đeo băng mà không cần tháo băng hoặc đến trung tâm chăm sóc vết thương. Ngoài ra, khả năng điều chỉnh WVTR theo mức dịch tiết của vết thương (hoặc có khả năng hấp thụ lớn mà không làm thay đổi WVTR) sẽ rất hữu ích. Tính năng này sẽ đảm bảo mức độ hydrat hóa tối ưu của bề mặt vết thương trong suốt quá trình điều trị."} +{"idx": 1870853, "en": "State-of-the-art methods for diminished reality propagate pixel information from a keyframe to subsequent frames for real-time inpainting. However, these approaches produce artifacts, if the scene geometry is not sufficiently planar. In this article, we present InpaintFusion, a new real-time method that extends inpainting to non-planar scenes by considering both color and depth information in the inpainting process. We use an RGB-D sensor for simultaneous localization and mapping, in order to both track the camera and obtain a surfel map in addition to RGB images. We use the RGB-D information in a cost function for both the color and the geometric appearance to derive a global optimization for simultaneous inpainting of color and depth. The inpainted depth is merged in a global map by depth fusion. For the final rendering, we project the map model into image space, where we can use it for effects such as relighting and stereo rendering of otherwise hidden structures. We demonstrate the capabilities of our method by comparing it to inpainting results with methods using planar geometric proxies.", "gemini": "Các phương pháp hiện đại để giảm thực tế truyền bá thông tin pixel từ một khung hình chính đến các khung hình tiếp theo để nội suy thời gian thực. Tuy nhiên, các cách tiếp cận này tạo ra hiện tượng nhiễu nếu hình học cảnh không đủ phẳng. Trong bài viết này, chúng tôi trình bày InpaintFusion, một phương pháp thời gian thực mới mở rộng nội suy sang các cảnh không phẳng bằng cách xem xét cả thông tin màu sắc và độ sâu trong quá trình nội suy. Chúng tôi sử dụng cảm biến RGB-D để định vị và lập bản đồ đồng thời, để theo dõi camera và lấy bản đồ bề mặt ngoài việc lấy hình ảnh RGB. Chúng tôi sử dụng thông tin RGB-D trong hàm chi phí cho cả màu sắc và hình dạng hình học để có được tối ưu hóa toàn cục để nội suy đồng thời màu sắc và độ sâu. Độ sâu được nội suy được hợp nhất trong một bản đồ toàn cục bằng cách hợp nhất độ sâu. Đối với kết xuất cuối cùng, chúng tôi chiếu mô hình bản đồ vào không gian hình ảnh, nơi chúng tôi có thể sử dụng nó cho các hiệu ứng như chiếu sáng lại và kết xuất âm thanh nổi của các cấu trúc ẩn khác. Chúng tôi chứng minh khả năng của phương pháp của mình bằng cách so sánh nó với kết quả nội suy với các phương pháp sử dụng các giá trị gần hình học phẳng."} +{"idx": 1870854, "en": "Axons of dopaminergic neurons innervate the striatum where they contribute to movement and reinforcement learning. Past work has shown that striatal GABA tonically inhibits dopamine release, but whether GABA-A receptors directly modulate transmission or act indirectly through circuit elements is unresolved. Here, we use whole-cell and perforated-patch recordings to test for GABA-A receptors on the main dopaminergic neuron axons and branching processes within the striatum of adult mice. Application of GABA depolarized axons, but also decreased the amplitude of axonal spikes, limited propagation and reduced striatal dopamine release. The mechanism of inhibition involved sodium channel inactivation and shunting. Lastly, we show the positive allosteric modulator diazepam enhanced GABA-A currents on dopaminergic axons and directly inhibited release, but also likely acts by reducing excitation from cholinergic interneurons. Thus, we reveal the mechanisms of GABA-A receptor modulation of dopamine release and provide new insights into the actions of benzodiazepines within the striatum.", "gemini": "Các sợi trục của các tế bào thần kinh dopaminergic chi phối thể vân, nơi chúng góp phần vào quá trình vận động và học tăng cường. Các nghiên cứu trước đây đã chỉ ra rằng GABA thể vân ức chế giải phóng dopamine, nhưng liệu các thụ thể GABA-A có điều chế trực tiếp quá trình truyền hay hoạt động gián tiếp thông qua các yếu tố mạch thì vẫn chưa được giải quyết. Ở đây, chúng tôi sử dụng các bản ghi toàn tế bào và bản ghi vá đục lỗ để kiểm tra các thụ thể GABA-A trên các sợi trục tế bào thần kinh dopaminergic chính và các quá trình phân nhánh trong thể vân của chuột trưởng thành. Việc sử dụng GABA làm khử cực các sợi trục, nhưng cũng làm giảm biên độ của các gai sợi trục, hạn chế sự lan truyền và làm giảm giải phóng dopamine thể vân. Cơ chế ức chế liên quan đến quá trình bất hoạt kênh natri và sự phân luồng. Cuối cùng, chúng tôi chỉ ra rằng chất điều biến tích cực diazepam làm tăng dòng GABA-A trên các sợi trục dopaminergic và trực tiếp ức chế giải phóng, nhưng cũng có khả năng hoạt động bằng cách giảm sự kích thích từ các tế bào thần kinh đệm cholinergic. Do đó, chúng tôi tiết lộ các cơ chế điều chế giải phóng dopamine của thụ thể GABA-A và cung cấp những hiểu biết mới về tác động của benzodiazepin trong thể vân."} +{"idx": 1870855, "en": "OBJECTIVE This work examines the claim made in the literature that the inverse problem associated with image reconstruction in sparse-view computed tomography (CT) can be solved with a convolutional neural network (CNN). METHODS Training, and testing image/data pairs are generated in a dedicated breast CT simulation for sparse-view sampling, using two different object models. The trained CNN is tested to see if images can be accurately recovered from their corresponding sparse-view data. For reference, the same sparse-view CT data is reconstructed by the use of constrained total-variation (TV) minimization (TVmin), which exploits sparsity in the gradient magnitude image (GMI). RESULTS There is a significant discrepancy between the image obtained with the CNN and the image that generated the data. TVmin is able to accurately reconstruct the test images. CONCLUSION We find that the sparse-view CT inverse problem cannot be solved for the particular published CNN-based methodology that we chose, and the particular object model that we tested. SIGNIFICANCE The inability of the CNN to solve the inverse problem associated with sparse-view CT, for the specific conditions of the presented simulation, draws into question similar unsupported claims being made for the use of CNNs and deep-learning to solve inverse problems in medical imaging.", "gemini": "MỤC TIÊU Nghiên cứu này xem xét tuyên bố được đưa ra trong tài liệu rằng vấn đề nghịch đảo liên quan đến tái tạo hình ảnh trong chụp cắt lớp vi tính (CT) chế độ xem thưa thớt có thể được giải quyết bằng mạng nơ-ron tích chập (CNN). PHƯƠNG PHÁP Các cặp hình ảnh/dữ liệu đào tạo và kiểm tra được tạo ra trong mô phỏng CT vú chuyên dụng để lấy mẫu chế độ xem thưa thớt, sử dụng hai mô hình đối tượng khác nhau. CNN được đào tạo được kiểm tra để xem liệu hình ảnh có thể được phục hồi chính xác từ dữ liệu chế độ xem thưa thớt tương ứng của chúng hay không. Để tham khảo, dữ liệu CT chế độ xem thưa thớt tương tự được tái tạo bằng cách sử dụng phép tối thiểu biến thiên toàn phần (TV) bị ràng buộc (TVmin), khai thác tính thưa thớt trong hình ảnh độ lớn của gradient (GMI). KẾT QUẢ Có sự khác biệt đáng kể giữa hình ảnh thu được bằng CNN và hình ảnh tạo ra dữ liệu. TVmin có khả năng tái tạo chính xác các hình ảnh thử nghiệm. KẾT LUẬN Chúng tôi thấy rằng vấn đề nghịch đảo CT chế độ xem thưa thớt không thể được giải quyết cho phương pháp luận cụ thể dựa trên CNN đã công bố mà chúng tôi đã chọn và mô hình đối tượng cụ thể mà chúng tôi đã thử nghiệm. Ý NGHĨA Khả năng không thể giải quyết vấn đề nghịch đảo liên quan đến CT chế độ xem thưa thớt của CNN, đối với các điều kiện cụ thể của mô phỏng được trình bày, đặt ra câu hỏi về những tuyên bố tương tự không được hỗ trợ đang được đưa ra cho việc sử dụng CNN và học sâu để giải quyết các vấn đề nghịch đảo trong hình ảnh y tế."} +{"idx": 1870856, "en": "Recent developments in the field of cellular therapeutics have indicated the potential of stem cell injections directly to the spinal cord. Injections require either open surgery or a Magnetic Resonance Imaging (MRI) guided injection. Needle positioning during MRI imaging is a significant hurdle to direct spinal injection, as the small target region and interlaminar space require high positioning accuracy. OBJECTIVE To improve both the procedure time and positioning accuracy, an MRI guided robotic needle positioning system is developed. METHODS The robot uses linear piezoelectric motors to directly drive a parallel plane positioning mechanism. Feedback is provided through MRI during the orientation procedure. Both accuracy and repeatability of the robot are characterized. RESULTS This system is found to be capable of repeatability below 51 μm. Needle endpoint error is limited by imaging modality, but is validated to 156 μm. CONCLUSION The reported robot and MRI image feedback system is capable of repeatable and accurate needle guide positioning. SIGNIFICANCE This high accuracy will result in a significant improvement to the workflow of spinal injection procedures.", "gemini": "Những phát triển gần đây trong lĩnh vực điều trị tế bào đã chỉ ra tiềm năng của việc tiêm tế bào gốc trực tiếp vào tủy sống. Tiêm cần phẫu thuật mở hoặc tiêm có hướng dẫn của Chụp cộng hưởng từ (MRI). Định vị kim trong quá trình chụp MRI là một rào cản đáng kể đối với việc tiêm trực tiếp vào tủy sống, vì vùng mục tiêu nhỏ và khoảng gian liên mạc đòi hỏi độ chính xác định vị cao. MỤC TIÊU Để cải thiện cả thời gian thủ thuật và độ chính xác định vị, một hệ thống định vị kim bằng robot có hướng dẫn của MRI đã được phát triển. PHƯƠNG PHÁP Robot sử dụng động cơ áp điện tuyến tính để trực tiếp điều khiển cơ chế định vị mặt phẳng song song. Phản hồi được cung cấp thông qua MRI trong quá trình định hướng. Cả độ chính xác và khả năng lặp lại của robot đều được đặc trưng. KẾT QUẢ Hệ thống này được phát hiện có khả năng lặp lại dưới 51 μm. Lỗi điểm cuối của kim bị giới hạn bởi phương thức hình ảnh, nhưng được xác thực là 156 μm. KẾT LUẬN Robot được báo cáo và hệ thống phản hồi hình ảnh MRI có khả năng định vị hướng dẫn kim chính xác và có thể lặp lại. Ý NGHĨA Độ chính xác cao này sẽ dẫn đến cải thiện đáng kể quy trình làm việc của các thủ thuật tiêm tủy sống."} +{"idx": 1870857, "en": "The deep-learning-based approaches which have been repeatedly proven to bring benefits to visual recognition tasks usually make a strong assumption that the training and test data are drawn from similar feature spaces and distributions. However, such an assumption may not always hold in various practical application scenarios on visual recognition tasks. Inspired by the hierarchical organization of deep feature representation that progressively leads tomore abstract features at higher layers of representations, we propose to tackle this problem with a novel feature learning framework, which is called GMFAD, with better generalization capability in a multi-layer perceptron manner. We first learn feature representations at the shallow layer where shareable underlying factors among domains (a subset of which could be relevant for each particular domain) can be explored. In particular, we propose to align the domain divergence between domain pair(s) by considering both inter-dimension and inter-sample correlations, which have been largely ignored by many crossdomain visual recognition methods. Subsequently, to learn more abstract information which could further benefit transferability, we propose to conduct feature disentanglement at the deep feature layer. Extensive experiments based on different visual recognition tasks demonstrate that our proposed framework can learn better transferable feature representation compared with state-of-the-art baselines.", "gemini": "Các phương pháp tiếp cận dựa trên học sâu đã được chứng minh nhiều lần mang lại lợi ích cho các nhiệm vụ nhận dạng hình ảnh thường đưa ra một giả định mạnh mẽ rằng dữ liệu đào tạo và thử nghiệm được rút ra từ các không gian và phân phối tính năng tương tự nhau. Tuy nhiên, giả định như vậy có thể không phải lúc nào cũng đúng trong các kịch bản ứng dụng thực tế khác nhau trên các nhiệm vụ nhận dạng hình ảnh. Lấy cảm hứng từ tổ chức phân cấp của biểu diễn tính năng sâu dần dần dẫn đến các tính năng trừu tượng hơn ở các lớp biểu diễn cao hơn, chúng tôi đề xuất giải quyết vấn đề này bằng một khuôn khổ học tính năng mới, được gọi là GMFAD, với khả năng tổng quát hóa tốt hơn theo cách nhận thức đa lớp. Đầu tiên, chúng tôi tìm hiểu các biểu diễn tính năng ở lớp nông, nơi có thể khám phá các yếu tố cơ bản có thể chia sẻ giữa các miền (một tập hợp con trong số đó có thể có liên quan đến từng miền cụ thể). Đặc biệt, chúng tôi đề xuất liên kết sự khác biệt giữa các miền giữa các cặp miền bằng cách xem xét cả tương quan giữa các chiều và giữa các mẫu, vốn đã bị nhiều phương pháp nhận dạng hình ảnh chéo miền bỏ qua phần lớn. Sau đó, để tìm hiểu thông tin trừu tượng hơn có thể mang lại lợi ích hơn nữa cho khả năng chuyển giao, chúng tôi đề xuất tiến hành phân tách tính năng ở lớp tính năng sâu. Các thí nghiệm mở rộng dựa trên các nhiệm vụ nhận dạng hình ảnh khác nhau chứng minh rằng khuôn khổ chúng tôi đề xuất có thể học được biểu diễn tính năng có thể chuyển giao tốt hơn so với các đường cơ sở hiện đại."} +{"idx": 1870858, "en": "Many learning algorithms such as kernel machines, nearest neighbors, clustering, or anomaly detection, are based on distances or similarities. Before similarities are used for training an actual machine learning model, we would like to verify that they are bound to meaningful patterns in the data. In this paper, we propose to make similarities interpretable by augmenting them with an explanation. We develop BiLRP, a scalable and theoretically founded method to systematically decompose the output of an already trained deep similarity model on pairs of input features. Our method can be expressed as a composition of LRP explanations, which were shown in previous works to scale to highly nonlinear models. Through an extensive set of experiments, we demonstrate that BiLRP robustly explains complex similarity models, e.g. built on VGG-16 deep neural network features. Additionally, we apply our method to an open problem in digital humanities: detailed assessment of similarity between historical documents such as astronomical tables. Here again, BiLRP provides insight and brings verifiability into a highly engineered and problem-specific similarity model.", "gemini": "Nhiều thuật toán học như máy kernel, lân cận gần nhất, cụm hoặc phát hiện dị thường dựa trên khoảng cách hoặc sự tương đồng. Trước khi sử dụng các điểm tương đồng để đào tạo mô hình học máy thực tế, chúng tôi muốn xác minh rằng chúng gắn liền với các mẫu có ý nghĩa trong dữ liệu. Trong bài báo này, chúng tôi đề xuất làm cho các điểm tương đồng có thể diễn giải được bằng cách tăng cường chúng bằng cách giải thích. Chúng tôi phát triển BiLRP, một phương pháp có thể mở rộng và có cơ sở lý thuyết để phân tích có hệ thống đầu ra của mô hình tương đồng sâu đã được đào tạo trên các cặp tính năng đầu vào. Phương pháp của chúng tôi có thể được biểu thị dưới dạng thành phần của các giải thích LRP, đã được thể hiện trong các công trình trước đây để mở rộng quy mô thành các mô hình phi tuyến tính cao. Thông qua một loạt các thí nghiệm mở rộng, chúng tôi chứng minh rằng BiLRP giải thích một cách mạnh mẽ các mô hình tương đồng phức tạp, ví dụ như được xây dựng trên các tính năng mạng nơ-ron sâu VGG-16. Ngoài ra, chúng tôi áp dụng phương pháp của mình cho một vấn đề mở trong nhân văn kỹ thuật số: đánh giá chi tiết về sự tương đồng giữa các tài liệu lịch sử như bảng thiên văn. Một lần nữa, BiLRP cung cấp thông tin chi tiết và mang lại khả năng xác minh vào một mô hình tương đồng có tính kỹ thuật cao và cụ thể cho từng vấn đề."} +{"idx": 1870859, "en": "In this paper, we propose a geometric neural network with edge-aware refinement (GeoNet++) to jointly predict both depth and surface normal maps from a single image. Building on top of two-stream CNNs, GeoNet++ captures the geometric relationships between depth and surface normals with the proposed depth-to-normal and normal-to-depth modules. In particular, the \"depth-to-normal\" module exploits the least square solution of estimating surface normals from depth to improve their quality, while the \"normal-to-depth\" module refines the depth map based on the constraints on surface normals through kernel regression. Boundary information is exploited via an edge-aware refinement module. GeoNet++ effectively predicts depth and surface normals with high 3D consistency and sharp boundaries resulting in better reconstructed 3D scenes. Note that GeoNet++ is generic and can be used in other depth/normal prediction frameworks to improve 3D reconstruction quality and pixel-wise accuracy of depth and surface normals. Furthermore, we propose a new 3D geometric metric (3DGM) for evaluating depth prediction in 3D. In contrast to current metrics that focus on evaluating pixel-wise error/accuracy, 3DGM measures whether the predicted depth can reconstruct high-quality 3D surface normals. This is a more natural metric for many 3D application domains. Our experiments on NYUD-V2 and KITTI demonstrate the effectiveness of our approach.", "gemini": "Trong bài báo này, chúng tôi đề xuất một mạng nơ-ron hình học với sự tinh chỉnh nhận biết cạnh (GeoNet++) để dự đoán đồng thời cả bản đồ độ sâu và bản đồ pháp tuyến bề mặt từ một hình ảnh duy nhất. Xây dựng dựa trên CNN hai luồng, GeoNet++ nắm bắt các mối quan hệ hình học giữa độ sâu và pháp tuyến bề mặt với các mô-đun độ sâu đến pháp tuyến và pháp tuyến đến độ sâu được đề xuất. Đặc biệt, mô-đun \"độ sâu đến pháp tuyến\" khai thác giải pháp bình phương nhỏ nhất để ước tính pháp tuyến bề mặt từ độ sâu để cải thiện chất lượng của chúng, trong khi mô-đun \"pháp tuyến đến độ sâu\" tinh chỉnh bản đồ độ sâu dựa trên các ràng buộc về pháp tuyến bề mặt thông qua hồi quy hạt nhân. Thông tin ranh giới được khai thác thông qua một mô-đun tinh chỉnh nhận biết cạnh. GeoNet++ dự đoán hiệu quả độ sâu và pháp tuyến bề mặt với độ nhất quán 3D cao và các ranh giới sắc nét dẫn đến các cảnh 3D được tái tạo tốt hơn. Lưu ý rằng GeoNet++ là chung chung và có thể được sử dụng trong các khung dự đoán độ sâu/pháp tuyến khác để cải thiện chất lượng tái tạo 3D và độ chính xác từng pixel của độ sâu và pháp tuyến bề mặt. Hơn nữa, chúng tôi đề xuất một số liệu hình học 3D mới (3DGM) để đánh giá dự đoán độ sâu trong 3D. Trái ngược với các số liệu hiện tại tập trung vào việc đánh giá lỗi/độ chính xác từng pixel, 3DGM đo lường xem độ sâu được dự đoán có thể tái tạo được các pháp tuyến bề mặt 3D chất lượng cao hay không. Đây là một số liệu tự nhiên hơn cho nhiều lĩnh vực ứng dụng 3D. Các thí nghiệm của chúng tôi trên các tập dữ liệu NYUD-V2 và KITTI chứng minh hiệu quả của phương pháp tiếp cận của chúng tôi."} +{"idx": 1870860, "en": "Generic object counting in natural scenes is a challenging computer vision problem. Existing approaches either rely on instance-level supervision or absolute count information to train a generic object counter. We introduce a partially supervised setting that significantly reduces the supervision level required for generic object counting. We propose two novel frameworks, named lower-count (LC) and reduced lower-count (RLC), to enable object counting under this setting. Our frameworks are built on a novel dual-branch architecture that has an image classification and a density branch. Our LC framework reduces the annotation cost due to multiple instances in an image by using only lower-count supervision for all object categories. Our RLC framework further reduces the annotation cost arising from large numbers of object categories in a dataset by only using lower-count supervision for a subset of categories and class-labels for the remaining ones. The RLC framework extends our dual-branch LC framework with a novel weight modulation layer and a category-independent density map prediction. Experiments are performed on COCO, Visual Genome and PASCAL 2007 datasets. Our frameworks perform on par with state-of-the-art approaches using higher levels of supervision. Additionally, we demonstrate the applicability of our LC supervised density map for image-level supervised instance segmentation.", "gemini": "Đếm đối tượng chung trong các cảnh tự nhiên là một vấn đề khó khăn của thị giác máy tính. Các phương pháp tiếp cận hiện có hoặc dựa vào giám sát cấp độ thể hiện hoặc thông tin số lượng tuyệt đối để đào tạo bộ đếm đối tượng chung. Chúng tôi giới thiệu một thiết lập được giám sát một phần giúp giảm đáng kể mức độ giám sát cần thiết để đếm đối tượng chung. Chúng tôi đề xuất hai khuôn khổ mới, có tên là số lượng thấp hơn (LC) và số lượng thấp hơn giảm (RLC), để cho phép đếm đối tượng trong thiết lập này. Các khuôn khổ của chúng tôi được xây dựng trên một kiến trúc nhánh kép mới có phân loại hình ảnh và nhánh mật độ. Khuôn khổ LC của chúng tôi làm giảm chi phí chú thích do nhiều thể hiện trong một hình ảnh bằng cách chỉ sử dụng giám sát số lượng thấp hơn cho tất cả các loại đối tượng. Khuôn khổ RLC của chúng tôi tiếp tục giảm chi phí chú thích phát sinh từ số lượng lớn các loại đối tượng trong một tập dữ liệu bằng cách chỉ sử dụng giám sát số lượng thấp hơn cho một tập hợp con các loại và nhãn lớp cho các loại còn lại. Khuôn khổ RLC mở rộng khuôn khổ LC nhánh kép của chúng tôi với một lớp điều chế trọng số mới và dự đoán bản đồ mật độ không phụ thuộc vào loại. Các thí nghiệm được thực hiện trên các tập dữ liệu COCO, Visual Genome và PASCAL 2007. Các khuôn khổ của chúng tôi thực hiện ngang bằng với các phương pháp tiếp cận hiện đại sử dụng các mức độ giám sát cao hơn. Ngoài ra, chúng tôi chứng minh khả năng áp dụng của bản đồ mật độ được giám sát LC của chúng tôi để phân đoạn thể hiện được giám sát cấp độ hình ảnh."} +{"idx": 1870861, "en": "Up to April 4, 2020, the novel coronavirus disease-2019 COVID-19 has affected more than 1 099000 patients and has become a major global health concern. World Health Organization (WHO) has defined COVID-19 as a global pandemic. Critical care ultrasound (CCUS) can rapidly acquire the image of lung and other organs and demonstrate the pathophysiological changes to guide precise therapy in COVID-19 pneumonia without radiation or interfering with personal protective equipment. In addition, the application of CCUS can cover the whole courses from the fever clinic to the intensive care unit to improve the treatment. We would like to present the CCUS features about COVID-19 pneumonia and share the application experience of CCUS in Wuhan, China, and hope it works for physicians worldwide to solve the problem and improve the outcome.", "gemini": "Tính đến ngày 4 tháng 4 năm 2020, bệnh do chủng vi-rút corona mới-2019 COVID-19 đã ảnh hưởng đến hơn 1.099.000 bệnh nhân và đã trở thành mối quan tâm lớn về sức khỏe toàn cầu. Tổ chức Y tế Thế giới (WHO) đã định nghĩa COVID-19 là một đại dịch toàn cầu. Siêu âm chăm sóc quan trọng (CCUS) có thể nhanh chóng thu được hình ảnh của phổi và các cơ quan khác và chứng minh những thay đổi bệnh sinh lý để hướng dẫn điều trị chính xác bệnh viêm phổi COVID-19 mà không cần bức xạ hoặc can thiệp vào thiết bị bảo vệ cá nhân. Ngoài ra, việc ứng dụng CCUS có thể bao quát toàn bộ quá trình từ phòng khám sốt đến khoa hồi sức tích cực để nâng cao hiệu quả điều trị. Chúng tôi muốn trình bày các đặc điểm của CCUS về viêm phổi COVID-19 và chia sẻ kinh nghiệm ứng dụng CCUS tại Vũ Hán, Trung Quốc, và hy vọng nó có hiệu quả đối với các bác sĩ trên toàn thế giới để giải quyết vấn đề và cải thiện kết quả."} +{"idx": 1870862, "en": "The use of wireless implanted medical devices (IMDs) is growing because they facilitate monitoring of patients at home and during normal activities, reduce the discomfort of patients, and reduce the likelihood of infection associated with trailing wires. Currently, radio frequency (RF) electromagnetic waves are the most commonly used method for communicating wirelessly with IMDs. However, due to the restrictions on the available bandwidth and the employable power, data rates of RF-based IMDs are limited to 267 kb/s. Considering standard definition video streaming requires data rates of 1.2 Mb/s and high definition requires 3 Mb/s, it is not possible to use the RF electromagnetic communications for high data rate communication applications such as video streaming. In this work, an alternative method that utilizes ultrasonic waves to relay information at high data rates is introduced. An advanced quadrature amplitude modulation (QAM) modem with phase-compensating, sparse decision feedback equalizer (DFE) is tailored to realize the full potential of the ultrasonic channel through biological tissues. The proposed system is tested in a variety of scenarios, including both simulations with finite impulse response (FIR) channel models, and real physical transmission experiments with ex vivo beef liver and pork chop samples as well as in situ rabbit abdomen. Consequently, the simulations demonstrated that video-capable data rates can be achieved with millimeter-sized transducers. Real physical experiments confirmed data rates of 6.7, 4.4, 4, and 3.2 Mb/s through water, ex vivo beef liver, ex vivo pork chop, and in situ rabbit abdomen, respectively.", "gemini": "Việc sử dụng các thiết bị y tế cấy ghép không dây (IMD) đang ngày càng phát triển vì chúng tạo điều kiện theo dõi bệnh nhân tại nhà và trong các hoạt động bình thường, giảm sự khó chịu của bệnh nhân và giảm khả năng nhiễm trùng liên quan đến dây dẫn. Hiện nay, sóng điện từ tần số vô tuyến (RF) là phương pháp được sử dụng phổ biến nhất để giao tiếp không dây với IMD. Tuy nhiên, do những hạn chế về băng thông khả dụng và công suất có thể sử dụng, tốc độ dữ liệu của IMD dựa trên RF bị giới hạn ở mức 267 kb/giây. Xem xét việc truyền phát video độ nét chuẩn yêu cầu tốc độ dữ liệu là 1,2 Mb/giây và độ nét cao yêu cầu 3 Mb/giây, không thể sử dụng thông tin liên lạc điện từ RF cho các ứng dụng truyền thông tốc độ dữ liệu cao như truyền phát video. Trong công trình này, một phương pháp thay thế sử dụng sóng siêu âm để truyền thông tin ở tốc độ dữ liệu cao đã được giới thiệu. Một modem điều chế biên độ vuông góc (QAM) tiên tiến với bù pha, bộ cân bằng phản hồi quyết định thưa thớt (DFE) được thiết kế riêng để nhận ra tiềm năng đầy đủ của kênh siêu âm thông qua các mô sinh học. Hệ thống được đề xuất được thử nghiệm trong nhiều tình huống khác nhau, bao gồm cả mô phỏng với các mô hình kênh đáp ứng xung hữu hạn (FIR) và các thí nghiệm truyền thực tế với các mẫu gan bò và sườn heo ngoài cơ thể cũng như bụng thỏ tại chỗ. Do đó, các mô phỏng đã chứng minh rằng tốc độ dữ liệu có khả năng truyền phát video có thể đạt được với các đầu dò có kích thước milimet. Các thí nghiệm vật lý thực tế đã xác nhận tốc độ dữ liệu là 6,7, 4,4, 4 và 3,2 Mb/giây thông qua nước, gan bò ngoài cơ thể, sườn heo ngoài cơ thể và bụng thỏ tại chỗ."} +{"idx": 1870863, "en": "The 3-D ultrasound imaging is essential for a wide range of clinical applications in diagnostic and interventional cardiology, radiology, and obstetrics for prenatal imaging. 3-D ultrasound imaging is also pivotal for advancing technical developments of emerging imaging technologies, such as elastography, blood flow imaging, functional ultrasound (fUS), and super-resolution microvessel imaging. At present, however, existing 3-D ultrasound imaging methods suffer from low imaging volume rate, suboptimal imaging quality, and high costs associated with 2-D ultrasound transducers. Here, we report a novel 3-D ultrasound imaging technique, fast acoustic steering via tilting electromechanical reflectors (FASTER), which provides both high imaging quality and fast imaging speed while at low cost. Capitalizing upon unique water immersible and fast-tilting microfabricated mirror to scan ultrafast plane waves in the elevational direction, FASTER is capable of high volume rate, large field-of-view (FOV) 3-D imaging with conventional 1-D transducers. In this article, we introduce the fundamental concepts of FASTER and present a series of calibration and validation studies for FASTER 3-D imaging. In a wire phantom and tissue-mimicking phantom study, we demonstrated that FASTER was capable of providing spatially accurate 3-D images with a 500-Hz imaging volume rate and an imaging FOV with a range of 48° (20 mm at 25-mm depth) in the elevational direction. We also showed that FASTER had comparable imaging quality with conventional mechanical translation-based 3-D imaging. The principles and results presented in this study establish the technical foundation for the new paradigm of high volume rate 3-D ultrasound imaging based on ultrafast plane waves and fast-tilting, water-immersible microfabricated mirrors.", "gemini": "Chụp ảnh siêu âm 3-D là rất cần thiết cho nhiều ứng dụng lâm sàng trong chẩn đoán và can thiệp tim mạch, chẩn đoán hình ảnh và sản khoa để chụp ảnh trước sinh. Chụp ảnh siêu âm 3-D cũng đóng vai trò quan trọng trong việc thúc đẩy phát triển kỹ thuật của các công nghệ hình ảnh mới nổi, như đàn hồi đồ, hình ảnh lưu lượng máu, siêu âm chức năng (fUS) và hình ảnh siêu vi mạch độ phân giải cao. Tuy nhiên, hiện nay, các phương pháp chụp ảnh siêu âm 3-D hiện có gặp phải tình trạng tốc độ khối lượng hình ảnh thấp, chất lượng hình ảnh không tối ưu và chi phí cao liên quan đến đầu dò siêu âm 2-D. Tại đây, chúng tôi báo cáo một kỹ thuật chụp ảnh siêu âm 3-D mới, điều khiển hướng âm thanh nhanh thông qua các bộ phản xạ cơ điện tử nghiêng (FASTER), cung cấp cả chất lượng hình ảnh cao và tốc độ chụp nhanh trong khi chi phí thấp. Tận dụng lợi thế của gương siêu nhỏ có thể ngâm trong nước và nghiêng nhanh độc đáo để quét các sóng phẳng siêu nhanh theo hướng nâng cao, FASTER có khả năng chụp ảnh 3-D có tốc độ khối lượng cao, trường nhìn (FOV) lớn với đầu dò 1-D thông thường. Trong bài viết này, chúng tôi giới thiệu các khái niệm cơ bản của FASTER và trình bày một loạt các nghiên cứu hiệu chuẩn và xác thực cho hình ảnh 3-D FASTER. Trong nghiên cứu mô phỏng dây và mô phỏng mô, chúng tôi đã chứng minh rằng FASTER có khả năng cung cấp hình ảnh 3-D chính xác về mặt không gian với tốc độ khối lượng hình ảnh 500 Hz và FOV hình ảnh có phạm vi 48° (20 mm ở độ sâu 25 mm) theo hướng nâng cao. Chúng tôi cũng chỉ ra rằng FASTER có chất lượng hình ảnh tương đương với hình ảnh 3-D dựa trên dịch chuyển cơ học thông thường. Các nguyên tắc và kết quả được trình bày trong nghiên cứu này thiết lập nền tảng kỹ thuật cho mô hình mới về chụp ảnh siêu âm 3-D tốc độ khối lượng cao dựa trên sóng phẳng siêu nhanh và gương siêu nhỏ có thể ngâm trong nước, nghiêng nhanh."} +{"idx": 1870864, "en": "Semantic segmentation with dense pixel-wise annotation has achieved excellent performance thanks to deep learning. However, the generalization of semantic segmentation in the wild remains challenging. In this paper, we address the problem of unsupervised domain adaptation (UDA) in semantic segmentation. Motivated by the fact that source and target domain have invariant semantic structures, we propose to exploit such invariance across domains by leveraging co-occurring patterns between pairwise pixels in the output of structured semantic segmentation. This is different from most existing approaches that attempt to adapt domains based on individual pixel-wise information in image, feature, or output level. Specifically, we perform domain adaptation on the affinity relationship between adjacent pixels termed affinity space of source and target domain. To this end, we develop two affinity space adaptation strategies: affinity space cleaning and adversarial affinity space alignment. Extensive experiments demonstrate that the proposed method achieves superior performance against some state-of-the-art methods on several challenging benchmarks for semantic segmentation across domains. The code is available at https://github.com/idealwei/ASANet.", "gemini": "Phân đoạn ngữ nghĩa với chú thích chi tiết theo từng pixel đã đạt được hiệu suất tuyệt vời nhờ học sâu. Tuy nhiên, việc khái quát hóa phân đoạn ngữ nghĩa trong tự nhiên vẫn còn nhiều thách thức. Trong bài báo này, chúng tôi giải quyết vấn đề thích ứng miền không giám sát (UDA) trong phân đoạn ngữ nghĩa. Dựa trên thực tế là miền nguồn và miền đích có cấu trúc ngữ nghĩa bất biến, chúng tôi đề xuất khai thác sự bất biến đó trên các miền bằng cách tận dụng các mẫu đồng thời giữa các pixel theo cặp trong đầu ra của phân đoạn ngữ nghĩa có cấu trúc. Điều này khác với hầu hết các phương pháp hiện có cố gắng thích ứng các miền dựa trên thông tin từng pixel trong hình ảnh, đặc điểm hoặc mức đầu ra. Cụ thể, chúng tôi thực hiện thích ứng miền trên mối quan hệ tương quan giữa các pixel liền kề được gọi là không gian tương quan của miền nguồn và miền đích. Để đạt được mục đích này, chúng tôi phát triển hai chiến lược thích ứng không gian tương quan: làm sạch không gian tương quan và liên kết không gian tương quan đối nghịch. Các thí nghiệm mở rộng chứng minh rằng phương pháp được đề xuất đạt hiệu suất vượt trội so với một số phương pháp tiên tiến trên một số chuẩn đánh giá đầy thách thức để phân đoạn ngữ nghĩa trên các miền. Mã có sẵn tại https://github.com/idealwei/ASANet."} +{"idx": 1870865, "en": "3D shape reconstruction from multiple hand-drawn sketches is an intriguing way to 3D shape modeling. Currently, state-of-the-art methods employ neural networks to learn a mapping from multiple sketches from arbitrary view angles to a 3D voxel grid. Because of the cubic complexity of 3D voxel grids, however, neural networks are hard to train and limited to low resolution reconstructions, which leads to a lack of geometric detail and low accuracy. To resolve this issue, we propose to reconstruct 3D shapes from multiple sketches using direct shape optimization (DSO), which does not involve deep learning models for direct voxel-based 3D shape generation. Specifically, we first leverage a conditional generative adversarial network (CGAN) to translate each sketch into an attenuance image that captures the predicted geometry from a given viewpoint. Then, DSO minimizes a project-and-compare loss to reconstruct the 3D shape such that it matches the predicted attenuance images from the view angles of all input sketches. Based on this, we further propose a progressive update approach to handle inconsistencies among a few hand-drawn sketches for the same 3D shape. Our experimental results show that our method significantly outperforms the state-of-the-art methods under widely used benchmarks and produces intuitive results in an interactive application.", "gemini": "Tái tạo hình dạng 3D từ nhiều bản phác thảo vẽ tay là một cách hấp dẫn để tạo mô hình hình dạng 3D. Hiện nay, các phương pháp tiên tiến sử dụng mạng nơ-ron để tìm hiểu ánh xạ từ nhiều bản phác thảo từ các góc nhìn tùy ý sang lưới voxel 3D. Tuy nhiên, do độ phức tạp khối lập phương của lưới voxel 3D, mạng nơ-ron rất khó đào tạo và chỉ giới hạn ở độ phân giải thấp, dẫn đến thiếu chi tiết hình học và độ chính xác thấp. Để giải quyết vấn đề này, chúng tôi đề xuất tái tạo hình dạng 3D từ nhiều bản phác thảo bằng cách sử dụng tối ưu hóa hình dạng trực tiếp (DSO), không liên quan đến các mô hình học sâu để tạo hình dạng 3D trực tiếp dựa trên voxel. Cụ thể, trước tiên chúng tôi sử dụng mạng đối nghịch sinh có điều kiện (CGAN) để chuyển đổi từng bản phác thảo thành một hình ảnh làm mờ nắm bắt hình học được dự đoán từ một góc nhìn nhất định. Sau đó, DSO giảm thiểu tổn thất dự án và so sánh để tái tạo hình dạng 3D sao cho nó khớp với các hình ảnh làm mờ được dự đoán từ các góc nhìn của tất cả các bản phác thảo đầu vào. Dựa trên điều này, chúng tôi tiếp tục đề xuất một phương pháp cập nhật tiến bộ để xử lý sự không nhất quán giữa một vài bản phác thảo vẽ tay cho cùng một hình dạng 3D. Kết quả thử nghiệm của chúng tôi cho thấy phương pháp của chúng tôi vượt trội đáng kể so với các phương pháp tiên tiến theo các chuẩn đánh giá được sử dụng rộng rãi và tạo ra các kết quả trực quan trong một ứng dụng tương tác."} +{"idx": 1870866, "en": "Handling deformation is one of the biggest challenges associated with point cloud registration. When deformation happens due to the motion of an animated object which actively changes its location and general shape, registration of two instances of the same object turns out to be a challenging task. The focus of this work is to address the problem by leveraging the complementary attributes of local and global geometric structures of the point clouds. We define an energy function which consists of local and global terms, as well as a semi-local term to model the intermediate level geometry of the point cloud. The local energy estimates the transformation parameters at the lowest level by assuming a reduced deformation model. The parameters are estimated in a closed form solution, which are then used to assign the initial probability of a stochastic model working at the intermediate level. The global energy term estimates the overall transformation parameters by minimizing a nonlinear least square function via Gauss-Newton optimization framework. The total energy is optimized in a block coordinate descent fashion, updating one term at a time while keeping others constant. Experiments on three publicly available datasets show that the method performs significantly better than several state-of-the-art algorithms in registering pairwise point cloud data.", "gemini": "Xử lý biến dạng là một trong những thách thức lớn nhất liên quan đến việc đăng ký đám mây điểm. Khi biến dạng xảy ra do chuyển động của một vật thể hoạt hình thay đổi tích cực vị trí và hình dạng chung của nó, việc đăng ký hai phiên bản của cùng một vật thể trở thành một nhiệm vụ đầy thách thức. Trọng tâm của công trình này là giải quyết vấn đề bằng cách tận dụng các thuộc tính bổ sung của cấu trúc hình học cục bộ và toàn cục của đám mây điểm. Chúng tôi xác định một hàm năng lượng bao gồm các thuật ngữ cục bộ và toàn cục, cũng như một thuật ngữ bán cục bộ để mô hình hóa hình học cấp trung gian của đám mây điểm. Năng lượng cục bộ ước tính các tham số biến đổi ở mức thấp nhất bằng cách giả định một mô hình biến dạng giảm. Các tham số được ước tính trong một giải pháp dạng đóng, sau đó được sử dụng để chỉ định xác suất ban đầu của một mô hình ngẫu nhiên hoạt động ở cấp trung gian. Thuật ngữ năng lượng toàn cục ước tính các tham số biến đổi tổng thể bằng cách giảm thiểu hàm bình phương ít nhất phi tuyến thông qua khung tối ưu hóa Gauss-Newton. Tổng năng lượng được tối ưu hóa theo kiểu giảm tọa độ khối, cập nhật từng thuật ngữ một lần trong khi giữ các thuật ngữ khác không đổi. Các thí nghiệm trên ba tập dữ liệu có sẵn công khai cho thấy phương pháp này hoạt động tốt hơn đáng kể so với một số thuật toán tiên tiến trong việc đăng ký dữ liệu đám mây điểm từng cặp."} +{"idx": 1870867, "en": "Rectilinear face recognition models suffer from severe performance degradation when applied to fisheye images captured by 360° back-to-back dual fisheye cameras. We propose a novel face rectification method to combat the effect of fisheye image distortion on face recognition. The method consists of a classification network and a restoration network specifically designed to handle the non-linear property of fisheye projection. The classification network classifies an input fisheye image according to its distortion level. The restoration network takes a distorted image as input and restores the rectilinear geometric structure of the face. The performance of the proposed method is tested on an end-to-end face recognition system constructed by integrating the proposed rectification method with a conventional rectilinear face recognition system. The face verification accuracy of the integrated system is 99.18% when tested on images in the synthetic Labeled Faces in the Wild (LFW) dataset and 95.70% for images in a real image dataset, resulting in an average accuracy improvement of 6.57% over the conventional face recognition system. For face identification, the average improvement over the conventional face recognition system is 4.51%.", "gemini": "Các mô hình nhận dạng khuôn mặt trực tuyến bị suy giảm hiệu suất nghiêm trọng khi áp dụng cho hình ảnh mắt cá được chụp bởi camera mắt cá kép 360° liên tiếp. Chúng tôi đề xuất một phương pháp chỉnh sửa khuôn mặt mới để chống lại tác động của sự biến dạng hình ảnh mắt cá đối với việc nhận dạng khuôn mặt. Phương pháp này bao gồm một mạng phân loại và một mạng khôi phục được thiết kế riêng để xử lý tính chất phi tuyến tính của phép chiếu mắt cá. Mạng phân loại phân loại một hình ảnh mắt cá đầu vào theo mức độ biến dạng của nó. Mạng khôi phục lấy một hình ảnh bị biến dạng làm đầu vào và khôi phục lại cấu trúc hình học trực tuyến của khuôn mặt. Hiệu suất của phương pháp được đề xuất được kiểm tra trên hệ thống nhận dạng khuôn mặt đầu cuối được xây dựng bằng cách tích hợp phương pháp chỉnh sửa được đề xuất với hệ thống nhận dạng khuôn mặt trực tuyến thông thường. Độ chính xác xác minh khuôn mặt của hệ thống tích hợp là 99,18% khi được kiểm tra trên các hình ảnh trong tập dữ liệu Khuôn mặt được gắn nhãn tổng hợp (LFW) và 95,70% đối với hình ảnh trong tập dữ liệu hình ảnh thực, dẫn đến độ chính xác trung bình cải thiện 6,57% so với hệ thống nhận dạng khuôn mặt thông thường. Đối với nhận dạng khuôn mặt, mức cải thiện trung bình so với hệ thống nhận dạng khuôn mặt thông thường là 4,51%."} +{"idx": 1870868, "en": "We present 3D virtual pancreatography (VP), a novel visualization procedure and application for non-invasive diagnosis and classification of pancreatic lesions, the precursors of pancreatic cancer. Currently, non-invasive screening of patients is performed through visual inspection of 2D axis-aligned CT images, though the relevant features are often not clearly visible nor automatically detected. VP is an end-to-end visual diagnosis system that includes: a machine learning based automatic segmentation of the pancreatic gland and the lesions, a semi-automatic approach to extract the primary pancreatic duct, a machine learning based automatic classification of lesions into four prominent types, and specialized 3D and 2D exploratory visualizations of the pancreas, lesions and surrounding anatomy. We combine volume rendering with pancreas- and lesion-centric visualizations and measurements for effective diagnosis. We designed VP through close collaboration and feedback from expert radiologists, and evaluated it on multiple real-world CT datasets with various pancreatic lesions and case studies examined by the expert radiologists.", "gemini": "Chúng tôi trình bày nội soi tụy ảo 3D (VP), một quy trình trực quan hóa mới và ứng dụng để chẩn đoán và phân loại không xâm lấn các tổn thương tụy, tiền thân của ung thư tụy. Hiện nay, việc sàng lọc không xâm lấn cho bệnh nhân được thực hiện thông qua việc kiểm tra trực quan hình ảnh CT căn chỉnh trục 2D, mặc dù các đặc điểm có liên quan thường không nhìn thấy rõ hoặc không được phát hiện tự động. VP là một hệ thống chẩn đoán trực quan đầu cuối bao gồm: phân đoạn tự động tuyến tụy và các tổn thương dựa trên học máy, một phương pháp bán tự động để trích xuất ống tụy chính, phân loại tự động các tổn thương dựa trên học máy thành bốn loại nổi bật và trực quan hóa khám phá 3D và 2D chuyên biệt của tụy, tổn thương và giải phẫu xung quanh. Chúng tôi kết hợp kết xuất khối lượng với các phép đo và hình ảnh trực quan tập trung vào tụy và tổn thương để chẩn đoán hiệu quả. Chúng tôi đã thiết kế VP thông qua sự hợp tác chặt chẽ và phản hồi từ các bác sĩ chuyên khoa về X quang và đánh giá VP trên nhiều tập dữ liệu CT thực tế có nhiều tổn thương tụy khác nhau và các nghiên cứu trường hợp do các bác sĩ chuyên khoa về X quang kiểm tra."} +{"idx": 1870869, "en": "Data movement, particularly access to the main memory, has been the bottleneck of most computing problems. Ray tracing is no exception. We propose an unconventional solution that combines a ray ordering scheme that minimizes access to the scene data with a large on-chip buffer acting as near-compute storage that is spread over multiple chips. We demonstrate the effectiveness of our approach by introducing Mach-RT (MAny CHip - Ray Tracing), a new hardware architecture for accelerating ray tracing. Extending the concept of dual streaming, we optimize the main memory accesses to a level that allows the same memory system to service multiple processor chips at the same time. While a multiple chip solution might seem to imply increased energy consumption as well, because of the reduced memory traffic we are able to demonstrate, performance increases while maintaining reasonable energy usage compared to academic and commercial architectures. This paper extends our previous work [1] with design space exploration of the L3 cache size, more detailed evaluation of energy and memory performance, a discussion of energy delay product, and a brief exploration of boards with 16 chips. We also introduce new treelet enqueueing logic for the predictive scheduler.", "gemini": "Việc di chuyển dữ liệu, đặc biệt là truy cập vào bộ nhớ chính, là nút thắt của hầu hết các vấn đề về điện toán. Ray tracing cũng không ngoại lệ. Chúng tôi đề xuất một giải pháp không thông thường kết hợp một lược đồ sắp xếp tia giúp giảm thiểu việc truy cập vào dữ liệu cảnh với một bộ đệm trên chip lớn đóng vai trò là bộ nhớ gần máy tính được trải rộng trên nhiều chip. Chúng tôi chứng minh hiệu quả của phương pháp tiếp cận của mình bằng cách giới thiệu Mach-RT (MAny CHip - Ray Tracing), một kiến trúc phần cứng mới để tăng tốc độ ray tracing. Mở rộng khái niệm truyền phát kép, chúng tôi tối ưu hóa các lần truy cập bộ nhớ chính đến mức cho phép cùng một hệ thống bộ nhớ phục vụ nhiều chip xử lý cùng một lúc. Mặc dù giải pháp nhiều chip có vẻ như cũng ngụ ý tăng mức tiêu thụ năng lượng, nhưng nhờ giảm lưu lượng bộ nhớ, chúng tôi có thể chứng minh rằng hiệu suất tăng lên trong khi vẫn duy trì mức sử dụng năng lượng hợp lý so với các kiến trúc học thuật và thương mại. Bài báo này mở rộng công trình trước đây của chúng tôi [1] với việc khám phá không gian thiết kế của kích thước bộ đệm L3, đánh giá chi tiết hơn về hiệu suất năng lượng và bộ nhớ, thảo luận về sản phẩm độ trễ năng lượng và khám phá ngắn gọn các bo mạch có 16 chip. Chúng tôi cũng giới thiệu logic xếp hàng cây mới cho trình lập lịch dự đoán."} +{"idx": 1870870, "en": "The performance of motor imagery (MI) based Brain-computer interfacing (BCI) is easily affected by noise and redundant information that exists in the multi-channel electroencephalogram (EEG). To solve this problem, many temporal and spatial feature based channel selection methods have been proposed. However, temporal and spatial features do not accurately reflect changes in the power of the oscillatory EEG. Thus, spectral features of MI-related EEG signals may be useful for channel selection. Bispectrum analysis is a technique developed for extracting non-linear and non-Gaussian information from non-linear and non-Gaussian signals. The features extracted from bispectrum analysis can provide frequency domain information about the EEG. Therefore, in this study, we propose a bispectrum-based channel selection (BCS) method for MI-based BCI. The proposed method uses the sum of logarithmic amplitudes (SLA) and the first order spectral moment (FOSM) features extracted from bispectrum analysis to select EEG channels without redundant information. Three public BCI competition datasets (BCI competition IV dataset 1, BCI competition III dataset IVa and BCI competition III dataset IIIa) were used to validate the effectiveness of our proposed method. The results indicate that our BCS method outperforms use of all channels (83.8% vs 69.4%, 86.3% vs 82.9% and 77.8% vs 68.2%, respectively). Furthermore, compared to the other state-of-the-art methods, our BCS method also can achieve significantly better classification accuracies for MI-based BCI (Wilcoxon signed test, p < 0.05).", "gemini": "Hiệu suất của giao diện não máy tính (BCI) dựa trên hình ảnh vận động (MI) dễ bị ảnh hưởng bởi nhiễu và thông tin dư thừa trong điện não đồ đa kênh (EEG). Để giải quyết vấn đề này, nhiều phương pháp chọn kênh dựa trên đặc điểm không gian và thời gian đã được đề xuất. Tuy nhiên, các đặc điểm không gian và thời gian không phản ánh chính xác những thay đổi về công suất của EEG dao động. Do đó, các đặc điểm phổ của tín hiệu EEG liên quan đến MI có thể hữu ích cho việc lựa chọn kênh. Phân tích phổ đôi là một kỹ thuật được phát triển để trích xuất thông tin phi tuyến tính và phi Gauss từ các tín hiệu phi tuyến tính và phi Gauss. Các đặc điểm trích xuất từ phân tích phổ đôi có thể cung cấp thông tin miền tần về EEG. Do đó, trong nghiên cứu này, chúng tôi đề xuất một phương pháp chọn kênh dựa trên phổ đôi (BCS) cho BCI dựa trên MI. Phương pháp được đề xuất sử dụng tổng các biên độ logarit (SLA) và các đặc điểm mô men phổ bậc nhất (FOSM) được trích xuất từ phân tích phổ đôi để chọn các kênh EEG không có thông tin dư thừa. Ba bộ dữ liệu cuộc thi BCI công khai (bộ dữ liệu cuộc thi BCI IV 1, bộ dữ liệu cuộc thi BCI III IVa và bộ dữ liệu cuộc thi BCI III IIIa) đã được sử dụng để xác thực hiệu quả của phương pháp chúng tôi đề xuất. Kết quả cho thấy phương pháp BCS của chúng tôi vượt trội hơn việc sử dụng tất cả các kênh (lần lượt là 83,8% so với 69,4%, 86,3% so với 82,9% và 77,8% so với 68,2%). Hơn nữa, so với các phương pháp tiên tiến khác, phương pháp BCS của chúng tôi cũng có thể đạt được độ chính xác phân loại tốt hơn đáng kể đối với BCI dựa trên MI (kiểm định có dấu Wilcoxon, p < 0,05)."} +{"idx": 1870871, "en": "Increasing number of experiments show that lncRNAs are involved in many biological processes, and their mutations and disorders are associated with many diseases. However, verifying the relationships between lncRNAs and diseases is time consuming and laborio. Searching for effective computational methods will contribute to our understanding of the underlying mechanisms of disease and identifying biomarkers of diseases. Therefore, we proposed a method called lncRNA-disease association prediction based on linear neighborhood similarity and unbalanced bi-random walk (LDA-LNSUBRW). Given that the known lncRNA-disease associations are rare, a pretreatment step should be performed to obtain the interaction possibility of unknown cases, so as to help us predict the potential associations. In the framework of leave-one-out cross-validation (LOOCV) and fivefold cross-validation (5-fold CV), LDA-LNSUBRW achieved effective performance with AUC of 0.8874 and 0.8632 ± 0.0051, respectively. The experimental results in this paper show that the proposed method is superior to five other state-of-the-art methods. In addition, case studies of three diseases (lung cancer, breast cancer, and osteosarcoma) were carried out to illustrate that LDA-LNSUBRW could predict the relevant lncRNAs.", "gemini": "Số lượng thí nghiệm ngày càng tăng cho thấy lncRNA liên quan đến nhiều quá trình sinh học và các đột biến và rối loạn của chúng có liên quan đến nhiều bệnh. Tuy nhiên, việc xác minh mối quan hệ giữa lncRNA và bệnh tật rất tốn thời gian và công sức. Việc tìm kiếm các phương pháp tính toán hiệu quả sẽ góp phần vào việc chúng ta hiểu được các cơ chế tiềm ẩn của bệnh và xác định các dấu ấn sinh học của bệnh. Do đó, chúng tôi đã đề xuất một phương pháp gọi là dự đoán mối liên quan giữa bệnh lncRNA dựa trên sự tương đồng lân cận tuyến tính và đi bộ ngẫu nhiên không cân bằng (LDA-LNSUBRW). Với giả định rằng các mối liên quan giữa lncRNA và bệnh đã biết là rất hiếm, nên thực hiện một bước tiền xử lý để có được khả năng tương tác của các trường hợp chưa biết, để giúp chúng ta dự đoán các mối liên quan tiềm ẩn. Trong khuôn khổ của xác thực chéo bỏ một (LOOCV) và xác thực chéo năm lần (CV 5 lần), LDA-LNSUBRW đạt hiệu suất hiệu quả với AUC lần lượt là 0,8874 và 0,8632 ± 0,0051. Kết quả thử nghiệm trong bài báo này cho thấy phương pháp được đề xuất vượt trội hơn năm phương pháp tiên tiến khác. Ngoài ra, các nghiên cứu trường hợp của ba bệnh (ung thư phổi, ung thư vú và ung thư xương) đã được thực hiện để minh họa rằng LDA-LNSUBRW có thể dự đoán các lncRNA có liên quan."} +{"idx": 1870872, "en": "In response to anticipated shortages of ventilators caused by the COVID-19 pandemic, many organizations have designed low-cost emergency ventilators. Many of these devices are pressure-cycled pneumatic ventilators, which are easy to produce but often do not include the sensing or alarm features found on commercial ventilators. This work reports a low-cost, easy-to-produce electronic sensor and alarm system for pressure-cycled ventilators that estimates clinically useful metrics such as pressure and respiratory rate and sounds an alarm when the ventilator malfunctions. A low-complexity signal processing algorithm uses a pair of nonlinear recursive envelope trackers to monitor the signal from an electronic pressure sensor connected to the patient airway. The algorithm, inspired by those used in hearing aids, requires little memory and performs only a few calculations on each sample so that it can run on nearly any microcontroller.", "gemini": "Để ứng phó với tình trạng thiếu máy thở do đại dịch COVID-19, nhiều tổ chức đã thiết kế máy thở khẩn cấp giá rẻ. Nhiều thiết bị trong số này là máy thở khí nén theo chu kỳ áp suất, dễ sản xuất nhưng thường không có các tính năng cảm biến hoặc báo động như trên máy thở thương mại. Công trình này báo cáo một hệ thống cảm biến và báo động điện tử giá rẻ, dễ sản xuất cho máy thở theo chu kỳ áp suất, hệ thống này ước tính các số liệu hữu ích về mặt lâm sàng như áp suất và nhịp hô hấp và phát ra báo động khi máy thở trục trặc. Một thuật toán xử lý tín hiệu có độ phức tạp thấp sử dụng một cặp bộ theo dõi bao tuyến tính phi tuyến để theo dõi tín hiệu từ cảm biến áp suất điện tử được kết nối với đường thở của bệnh nhân. Thuật toán này, được lấy cảm hứng từ những thuật toán được sử dụng trong máy trợ thính, yêu cầu ít bộ nhớ và chỉ thực hiện một vài phép tính trên mỗi mẫu để có thể chạy trên hầu hết mọi bộ vi điều khiển."} +{"idx": 1870873, "en": "Matching and registration between aerial images and prestored road landmarks are critical techniques to enhance unmanned aerial system (UAS) navigation in the global positioning system (GPS)-denied urban environments. Current registration processes typically consist of two separate stages of road extraction and road registration. These two-stage registration approaches are time-consuming and less robust to noise. To that end, in this article, we, for the first time, investigate the problem of end-to-end Aerial-Road registration. Using deep learning, we develop a novel attention-based neural network architecture for Aerial-Road registration. In this model, we construct two-branch neural networks with shared weights to map two input images into a common embedding space. Besides, considering that road features are sparsely distributed in images, we incorporate a novel multibranch attention module to filter out false descriptor matches from the indiscriminative background in order to improve registration accuracy. Finally, the results from extensive experiments show that compared with state-of-the-art approaches, the mean absolute errors of our approach in rotation angle and the translations in the x - and y -directions are reduced down by a factor of 1.24, 1.38, and 1.44, respectively. Furthermore, as a byproduct, our experimental results prove the feasibility of a neural network multitask learning approach to simultaneously achieve accurate Aerial-Road matching and registration, thus providing an efficient and accurate UAS geolocalization.", "gemini": "Việc khớp và đăng ký giữa ảnh trên không và các điểm mốc đường bộ được lưu trữ trước là các kỹ thuật quan trọng để nâng cao khả năng điều hướng của hệ thống máy bay không người lái (UAS) trong các môi trường đô thị không có hệ thống định vị toàn cầu (GPS). Các quy trình đăng ký hiện tại thường bao gồm hai giai đoạn riêng biệt là trích xuất đường và đăng ký đường. Các phương pháp đăng ký hai giai đoạn này rất tốn thời gian và ít mạnh mẽ hơn đối với nhiễu. Vì vậy, trong bài viết này, lần đầu tiên chúng tôi nghiên cứu vấn đề đăng ký Đường trên không từ đầu đến cuối. Sử dụng học sâu, chúng tôi phát triển một kiến trúc mạng nơ-ron mới dựa trên sự chú ý cho việc đăng ký Đường trên không. Trong mô hình này, chúng tôi xây dựng các mạng nơ-ron hai nhánh có trọng số được chia sẻ để ánh xạ hai ảnh đầu vào vào một không gian nhúng chung. Bên cạnh đó, khi xem xét rằng các đặc điểm đường được phân bổ thưa thớt trong ảnh, chúng tôi kết hợp một mô-đun chú ý đa nhánh mới để lọc ra các kết quả khớp mô tả sai từ nền không phân biệt để cải thiện độ chính xác của đăng ký. Cuối cùng, kết quả từ các thí nghiệm mở rộng cho thấy rằng so với các phương pháp tiếp cận hiện đại, lỗi tuyệt đối trung bình của phương pháp tiếp cận của chúng tôi theo góc quay và phép tịnh tiến theo hướng x và y giảm xuống lần lượt là 1,24, 1,38 và 1,44. Hơn nữa, như một sản phẩm phụ, kết quả thử nghiệm của chúng tôi chứng minh tính khả thi của phương pháp học đa nhiệm mạng nơ-ron để đồng thời đạt được việc khớp và đăng ký Đường trên không chính xác, do đó cung cấp một địa lý hóa UAS hiệu quả và chính xác."} +{"idx": 1870874, "en": "We present a three-step method to perform system identification and optimal control of nonlinear systems. Our approach is mainly data-driven and does not require active excitation of the system to perform system identification. In particular, it is designed for systems for which only historical data under closed-loop control are available and where historical control commands exhibit low variability. In the first step, simple simulation models of the system are built and run under various conditions. In the second step, a neural network architecture is extensively trained on the simulation outputs to learn the system physics and retrained with historical data from the real system with stopping rules. These constraints avoid overfitting that arises by fitting closed-loop controlled systems. By doing so, we obtain one (or many) system model(s), represented by this architecture, whose behavior can be chosen to match more or less the real system. Finally, state-of-the-art reinforcement learning with a variant of domain randomization and distributed learning is used for optimal control of the system. We first illustrate the model identification strategy with a simple example, the pendulum with external torque. We then apply our method to model and optimize the control of a large building facility located in Switzerland. Simulation results demonstrate that this approach generates stable functional controllers that outperform on comfort and energy benchmark rule-based controllers.", "gemini": "Chúng tôi trình bày một phương pháp ba bước để thực hiện nhận dạng hệ thống và kiểm soát tối ưu các hệ thống phi tuyến tính. Phương pháp tiếp cận của chúng tôi chủ yếu dựa trên dữ liệu và không yêu cầu kích thích chủ động của hệ thống để thực hiện nhận dạng hệ thống. Đặc biệt, nó được thiết kế cho các hệ thống mà chỉ có dữ liệu lịch sử dưới dạng điều khiển vòng kín và trong đó các lệnh điều khiển lịch sử thể hiện tính biến thiên thấp. Trong bước đầu tiên, các mô hình mô phỏng đơn giản của hệ thống được xây dựng và chạy trong các điều kiện khác nhau. Trong bước thứ hai, một kiến trúc mạng nơ-ron được đào tạo rộng rãi trên các đầu ra mô phỏng để tìm hiểu vật lý của hệ thống và được đào tạo lại bằng dữ liệu lịch sử từ hệ thống thực với các quy tắc dừng. Những ràng buộc này tránh được sự quá khớp phát sinh khi lắp các hệ thống điều khiển vòng kín. Bằng cách đó, chúng tôi thu được một (hoặc nhiều) mô hình hệ thống, được biểu diễn bởi kiến trúc này, mà hành vi của chúng có thể được chọn để phù hợp ít nhiều với hệ thống thực. Cuối cùng, việc học tăng cường hiện đại với một biến thể của ngẫu nhiên hóa miền và học phân tán được sử dụng để kiểm soát tối ưu hệ thống. Đầu tiên, chúng tôi minh họa chiến lược nhận dạng mô hình bằng một ví dụ đơn giản, con lắc với mô-men xoắn bên ngoài. Sau đó, chúng tôi áp dụng phương pháp của mình để mô hình hóa và tối ưu hóa việc kiểm soát một cơ sở tòa nhà lớn nằm ở Thụy Sĩ. Kết quả mô phỏng chứng minh rằng phương pháp tiếp cận này tạo ra các bộ điều khiển chức năng ổn định, hoạt động tốt hơn về sự thoải mái và tiêu chuẩn năng lượng so với các bộ điều khiển dựa trên quy tắc."} +{"idx": 1870875, "en": "Identifying cancer drivers is a crucial challenge to explain the underlying mechanisms of cancer development. There are many methods to identify cancer drivers based on the single mutation site or the entire gene. But they ignore a large number of functional elements with medium in size. It is hypothesized that mutations occurring in different regions of the protein sequence have different effects on the progression of cancer. Here, we develop a novel functional region driver(frDriver) identification method based on Bayesian probability and multiple linear regression models to identify protein regions that can regulate gene expression levels and have high functional impact potential. Combining gene expression data and somatic mutation data, with functional impact scores(SIFT, PROVEAN) as a priori knowledge, we identified cancer driver regions that are most accurate in predicting gene expression levels. We evaluated the performance of frDriver on the BRCA and GBM datasets from TCGA. The results showed that frDriver identified known cancer drivers and outperformed the other three state-of-the-art methods(eDriver, ActiveDriver and OncodriveCLUST). In addition, we performed KEGG pathway and GO term enrichment analysis, and the results indicated that the cancer drivers predicted by frDriver were related to processes such as cancer formation and gene regulation.", "gemini": "Xác định các trình điều khiển ung thư là một thách thức rất quan trọng để giải thích các cơ chế cơ bản của sự phát triển ung thư. Có nhiều phương pháp để xác định các trình điều khiển ung thư dựa trên vị trí đột biến đơn lẻ hoặc toàn bộ gen. Nhưng chúng bỏ qua một số lượng lớn các yếu tố chức năng có kích thước trung bình. Người ta đưa ra giả thuyết rằng các đột biến xảy ra ở các vùng khác nhau của trình tự protein có tác động khác nhau đến quá trình tiến triển của ung thư. Ở đây, chúng tôi phát triển một phương pháp nhận dạng trình điều khiển vùng chức năng mới (frDriver) dựa trên xác suất Bayes và các mô hình hồi quy tuyến tính bội để xác định các vùng protein có thể điều chỉnh mức độ biểu hiện gen và có tiềm năng tác động chức năng cao. Kết hợp dữ liệu biểu hiện gen và dữ liệu đột biến soma, với điểm tác động chức năng (SIFT, PROVEAN) như kiến thức tiên nghiệm, chúng tôi đã xác định các vùng điều khiển ung thư chính xác nhất trong việc dự đoán mức độ biểu hiện gen. Chúng tôi đã đánh giá hiệu suất của frDriver trên các tập dữ liệu BRCA và GBM từ TCGA. Kết quả cho thấy frDriver đã xác định được các trình điều khiển ung thư đã biết và vượt trội hơn ba phương pháp hiện đại khác (eDriver, ActiveDriver và OncodriveCLUST). Ngoài ra, chúng tôi đã thực hiện phân tích làm giàu thuật ngữ GO và con đường KEGG và kết quả cho thấy các trình điều khiển ung thư do frDriver dự đoán có liên quan đến các quá trình như hình thành ung thư và điều hòa gen."} +{"idx": 1870876, "en": "This article is concerned with the challenge of guaranteeing output constraints for fault-tolerant control (FTC) of a class of unknown multi-input single-output (MISO) nonlinear systems in the presence of actuator faults. Most industrial systems are equipped with redundant actuators and a fault detection-isolation mechanism for accommodating unexpected actuator faults. To simplify the system design and reduce the risk of false alarm or missed detection brought by the detection unit, a learning-based switching function scheme is proposed to automatically activate different sets of actuators in a rotational manner without human intervention. By this means, no explicit fault detection mechanism is needed. An additional step has been made to guarantee that the system output remains in user-defined time-varying asymmetric output constraints all the time during the occurrence of failures by utilizing error transformation techniques. The stability of the transformed system can equivalently deliver the result that the original system output stays in the required bounds. Hence, system crash or further catastrophic outcomes can be avoided. A neural network is integrated to embody the adaptive FTC design for dealing with unknown system dynamics. The dynamic surface control (DSC) technique is also invoked to decrease complexity. Furthermore, the stability analysis is carried out by the standard Lyapunov approach to guarantee that all the signals of the closed-loop system are semiglobally uniformly ultimately bounded. Finally, the simulation results are provided to verify the effectiveness of the proposed scheme.", "gemini": "Bài viết này liên quan đến thách thức đảm bảo các ràng buộc đầu ra cho điều khiển chịu lỗi (FTC) của một lớp hệ thống phi tuyến tính đầu vào nhiều đầu ra đơn (MISO) không xác định khi có lỗi bộ truyền động. Hầu hết các hệ thống công nghiệp đều được trang bị các bộ truyền động dự phòng và cơ chế cô lập phát hiện lỗi để xử lý các lỗi bộ truyền động bất ngờ. Để đơn giản hóa thiết kế hệ thống và giảm nguy cơ báo động giả hoặc bỏ sót phát hiện do đơn vị phát hiện gây ra, một lược đồ hàm chuyển mạch dựa trên học được đề xuất để tự động kích hoạt các bộ truyền động khác nhau theo cách xoay vòng mà không cần sự can thiệp của con người. Bằng cách này, không cần cơ chế phát hiện lỗi rõ ràng. Một bước bổ sung đã được thực hiện để đảm bảo rằng đầu ra của hệ thống luôn nằm trong các ràng buộc đầu ra không đối xứng theo thời gian do người dùng xác định mọi lúc trong quá trình xảy ra lỗi bằng cách sử dụng các kỹ thuật biến đổi lỗi. Độ ổn định của hệ thống đã biến đổi có thể tương đương cung cấp kết quả là đầu ra hệ thống ban đầu nằm trong các giới hạn yêu cầu. Do đó, có thể tránh được sự cố hệ thống hoặc các hậu quả thảm khốc hơn nữa. Một mạng lưới thần kinh được tích hợp để thể hiện thiết kế FTC thích ứng để xử lý động lực hệ thống không xác định. Kỹ thuật điều khiển bề mặt động (DSC) cũng được sử dụng để giảm độ phức tạp. Hơn nữa, phân tích độ ổn định được thực hiện theo phương pháp Lyapunov chuẩn để đảm bảo rằng tất cả các tín hiệu của hệ thống vòng kín đều bị chặn một cách đồng đều bán toàn cục. Cuối cùng, các kết quả mô phỏng được cung cấp để xác minh hiệu quả của lược đồ được đề xuất."} +{"idx": 1870877, "en": "Electronic stethoscopes offer several advantages over conventional acoustic stethoscopes, including noise reduction, increased amplification, and ability to store and transmit sounds. However, the acoustical characteristics of electronic and acoustic stethoscopes can differ significantly, introducing a barrier for clinicians to transition to electronic stethoscopes. This work proposes a method to process lung sounds recorded by an electronic stethoscope, such that the sounds are perceived to have been captured by an acoustic stethoscope. The proposed method calculates an electronic-to-acoustic stethoscope filter by measuring the difference between the average frequency responses of an acoustic and an electronic stethoscope to multiple lung sounds. To validate the method, a change detection experiment was conducted with 51 medical professionals to compare filtered electronic, unfiltered electronic, and acoustic stethoscope lung sounds. Participants were asked to detect when transitions occurred in sounds comprising several sections of the three types of recordings. Transitions between the filtered electronic and acoustic stethoscope sections were detected, on average, by chance (sensitivity index equal to zero) and also detected significantly less than transitions between the unfiltered electronic and acoustic stethoscope sections ( ), demonstrating the effectiveness of the method to filter electronic stethoscopes to mimic an acoustic stethoscope. This processing could incentivize clinicians to adopt electronic stethoscopes by providing a means to shift between the sound characteristics of acoustic and electronic stethoscopes in a single device, allowing for a faster transition to new technology and greater appreciation for the electronic sound quality.", "gemini": "Ống nghe điện tử có một số ưu điểm so với ống nghe cơ học thông thường, bao gồm giảm tiếng ồn, tăng độ khuếch đại và khả năng lưu trữ và truyền âm thanh. Tuy nhiên, các đặc tính âm học của ống nghe điện tử và ống nghe cơ học có thể khác nhau đáng kể, tạo ra rào cản khiến các bác sĩ lâm sàng chuyển sang sử dụng ống nghe điện tử. Nghiên cứu này đề xuất một phương pháp để xử lý âm thanh phổi được ghi lại bằng ống nghe điện tử, sao cho âm thanh được cảm nhận như được thu lại bằng ống nghe cơ học. Phương pháp được đề xuất tính toán bộ lọc ống nghe điện tử sang ống nghe cơ học bằng cách đo sự khác biệt giữa các đáp ứng tần số trung bình của ống nghe cơ học và ống nghe điện tử đối với nhiều âm thanh phổi. Để xác nhận phương pháp, một thí nghiệm phát hiện thay đổi đã được tiến hành với 51 chuyên gia y tế để so sánh âm thanh phổi của ống nghe điện tử đã lọc, ống nghe điện tử chưa lọc và ống nghe cơ học. Những người tham gia được yêu cầu phát hiện khi có sự chuyển đổi xảy ra trong âm thanh gồm một số phần của ba loại bản ghi. Sự chuyển đổi giữa các phần ống nghe điện tử đã lọc và ống nghe cơ học được phát hiện, trung bình là ngẫu nhiên (chỉ số độ nhạy bằng không) và cũng được phát hiện ít hơn đáng kể so với sự chuyển đổi giữa các phần ống nghe điện tử và ống nghe cơ học chưa lọc ( ), chứng minh hiệu quả của phương pháp lọc ống nghe điện tử để bắt chước ống nghe cơ học. Việc xử lý này có thể khuyến khích các bác sĩ lâm sàng sử dụng ống nghe điện tử bằng cách cung cấp phương tiện chuyển đổi giữa các đặc tính âm thanh của ống nghe cơ học và ống nghe điện tử trong một thiết bị duy nhất, cho phép chuyển đổi nhanh hơn sang công nghệ mới và đánh giá cao hơn chất lượng âm thanh điện tử."} +{"idx": 1870878, "en": "The main aim of this work is to identify alterations in the morphology of the pulse photoplethysmogram (PPG) signal, due to the exposure of the subjects to a hyperbaric environment. Additionally, their Pulse Rate Variability (PRV) is analysed to characterise the response of their Autonomic Nervous System (ANS). To do that, 28 volunteers are introduced into a hyperbaric chamber and five sequential stages with different atmospheric pressures from 1 atm to 5 atm are performed. In this work, nineteen morphological parameters of the PPG signal are analysed: the pulse amplitude; eight parameters related to pulse width; eight parameters related to pulse area; and the two two pulse slopes. Also, classical time and frequency parameters of PRV are computed. Notable widening of the pulses width is observed in the stages analysed. The PPG area increases with pressure, with no significant changes when the initial pressure is recovered. These changes in PPG waveform may be caused by an increase in the systemic vascular resistance as a consequence of of vasoconstriction in the extremities, suggesting a sympathetic activation. However, the PRV results show an augmented parasympathetic activity and a reduction in the parameters that characterise the sympathetic response. So, only a sympathetic activation is detected in the peripheral region, as reflected by PPG morphology. The information regarding the ANS and the cardiovascular response that can be extracted from the PPG signal, as well as its compatibility with wet conditions make this signal the most suitable for studying the physiological response in hyperbaric environments.", "gemini": "Mục đích chính của công trình này là xác định những thay đổi về mặt hình thái của tín hiệu quang điện xung (PPG) do những đối tượng tiếp xúc với môi trường tăng áp. Ngoài ra, Biến thiên nhịp xung (PRV) của họ được phân tích để mô tả phản ứng của Hệ thần kinh tự chủ (ANS) của họ. Để làm được điều đó, 28 tình nguyện viên được đưa vào buồng tăng áp và năm giai đoạn tuần tự với áp suất khí quyển khác nhau từ 1 atm đến 5 atm được thực hiện. Trong công trình này, mười chín thông số hình thái của tín hiệu PPG được phân tích: biên độ xung; tám thông số liên quan đến chiều rộng xung; tám thông số liên quan đến diện tích xung; và hai độ dốc xung. Ngoài ra, các thông số thời gian và tần số cổ điển của PRV được tính toán. Sự mở rộng đáng kể về chiều rộng xung được quan sát thấy trong các giai đoạn được phân tích. Diện tích PPG tăng theo áp suất, không có thay đổi đáng kể khi áp suất ban đầu được phục hồi. Những thay đổi này trong dạng sóng PPG có thể là do sự gia tăng sức cản mạch máu toàn thân do co mạch ở các chi, cho thấy sự kích hoạt giao cảm. Tuy nhiên, kết quả PRV cho thấy hoạt động phó giao cảm tăng lên và giảm các thông số đặc trưng cho phản ứng giao cảm. Vì vậy, chỉ có sự kích hoạt giao cảm được phát hiện ở vùng ngoại vi, như phản ánh trong hình thái PPG. Thông tin liên quan đến ANS và phản ứng tim mạch có thể được trích xuất từ tín hiệu PPG, cũng như khả năng tương thích của nó với các điều kiện ẩm ướt khiến tín hiệu này phù hợp nhất để nghiên cứu phản ứng sinh lý trong môi trường tăng áp."} +{"idx": 1870879, "en": "Objectives Hearing impairment and ear diseases are among the world's leading causes of years lived with disability. Ear disorders are a major health problem in developing countries like India. The present research had been planned to screen the primary school children for ear morbidity and to study the various factors associated with ear morbidities so that corrective intervention can be initiated at the earliest. Methods A cross-sectional study was conducted among all primary school children of two schools in Aliganj, Delhi. Complete enumeration of all the school children of these two primary schools was planned for this study. Modified version of World Health Organization's (WHO's) \"Ten Question Plus\" screening instrument was used, followed by Otoscopy examination, in all children for confirmation of ear disease, if any. Descriptive data analysis was performed. Results Out of the total 430 study subjects, ear morbidities were found in 198 (46.0%) study participants. Presence of ear morbidity showed significant association with overcrowding, absence of a separate kitchen, passive smoking, feeding young child in lying down position, upper respiratory tract infection (URI)˃3 episodes/year and allergy (p<0.05). Conclusions This study has highlighted the need to detect ear diseases at the earliest thereby avoiding complications. The health examination in the schools must include screening students for ear diseases to reduce the burden of hearing disability. Inputs from the study may be used for effective prioritization and planning for prevention and management of ear diseases at the primary school level.", "gemini": "Mục tiêu Giảm thính lực và các bệnh về tai là một trong những nguyên nhân hàng đầu gây ra tình trạng sống chung với khuyết tật trên thế giới. Các rối loạn về tai là một vấn đề sức khỏe lớn ở các nước đang phát triển như Ấn Độ. Nghiên cứu hiện tại đã được lập kế hoạch để sàng lọc trẻ em tiểu học về tình trạng bệnh tai và nghiên cứu các yếu tố khác nhau liên quan đến bệnh tai để có thể bắt đầu can thiệp khắc phục sớm nhất. Phương pháp Một nghiên cứu cắt ngang đã được tiến hành trên tất cả trẻ em tiểu học của hai trường ở Aliganj, Delhi. Kế hoạch của nghiên cứu này là thống kê đầy đủ tất cả trẻ em trong hai trường tiểu học này. Phiên bản sửa đổi của công cụ sàng lọc \"Mười câu hỏi cộng\" của Tổ chức Y tế Thế giới (WHO) đã được sử dụng, sau đó là khám bằng ống soi tai ở tất cả trẻ em để xác nhận bệnh tai, nếu có. Đã thực hiện phân tích dữ liệu mô tả. Kết quả Trong tổng số 430 đối tượng nghiên cứu, có 198 (46,0%) người tham gia nghiên cứu mắc bệnh về tai. Tình trạng bệnh tai có liên quan đáng kể đến tình trạng quá tải, không có bếp riêng, hút thuốc thụ động, cho trẻ nhỏ bú ở tư thế nằm, nhiễm trùng đường hô hấp trên (URI)˃3 đợt/năm và dị ứng (p<0,05). Kết luận Nghiên cứu này đã nhấn mạnh đến nhu cầu phát hiện sớm các bệnh về tai, do đó tránh được các biến chứng. Khám sức khỏe tại các trường học phải bao gồm sàng lọc bệnh tai cho học sinh để giảm gánh nặng khuyết tật về thính lực. Các dữ liệu đầu vào từ nghiên cứu có thể được sử dụng để lập kế hoạch và ưu tiên hiệu quả cho công tác phòng ngừa và quản lý các bệnh về tai ở cấp tiểu học."} +{"idx": 1870880, "en": "Objective The Centers for Disease Control & Prevention (CDC) recommends that the human papillomavirus (HPV) vaccine series be initiated at ages 11-12 years, but many children never follow-through with the series. By examining differences in clinic-specific vaccine series follow-through rates across four clinics, we aimed to identify best practices related to communication around the vaccine and pathways to follow-through. Methods We used the electronic medical record (EMR) to retrospectively analyze HPV vaccine follow-through at four clinic settings. We limited the sample to children ages 11-17 who received a dose of the vaccine series at any of the clinics between January 2015 and June 2018. The primary outcome was follow-through of the HPV vaccine series within 18 months of initiation. Results A total of 3,813 patients were included in this study, 29% of which followed through with the HPV vaccine series. There was significant variability of vaccine follow-through among the clinics (p<0.001), with the Med/Peds clinic having the highest rate of follow-through (32%). After adjusting for confounding variables, multivariable analysis found that Med/Peds and Family Medicine had higher odds of HPV vaccine series follow-through than the Pediatrics clinic. Conclusions We found that the likelihood of vaccine series follow-through was highest when the series was started in the Med/Peds and Family Medicine clinics, compared to Pediatrics and Adolescent Medicine. These results suggest that further qualitative research is needed to understand what communication strategies are most effective at facilitating HPV vaccine uptake among adolescents, and how the most effective strategies can be shared among clinics.", "gemini": "Mục tiêu Trung tâm Kiểm soát và Phòng ngừa Dịch bệnh (CDC) khuyến cáo rằng nên bắt đầu tiêm vắc-xin vi-rút gây u nhú ở người (HPV) ở độ tuổi 11-12, nhưng nhiều trẻ em không bao giờ tiêm đủ liều. Bằng cách kiểm tra sự khác biệt về tỷ lệ tiêm đủ liều vắc-xin theo từng phòng khám tại bốn phòng khám, chúng tôi nhằm mục đích xác định các hoạt động thực hành tốt nhất liên quan đến truyền thông xung quanh vắc-xin và các con đường để tiêm đủ liều. Phương pháp Chúng tôi đã sử dụng hồ sơ bệnh án điện tử (EMR) để phân tích hồi cứu việc tiêm đủ liều vắc-xin HPV tại bốn cơ sở phòng khám. Chúng tôi giới hạn mẫu đối với trẻ em từ 11-17 tuổi đã tiêm một liều vắc-xin tại bất kỳ phòng khám nào trong khoảng thời gian từ tháng 1 năm 2015 đến tháng 6 năm 2018. Kết quả chính là tiêm đủ liều vắc-xin HPV trong vòng 18 tháng sau khi bắt đầu. Kết quả Tổng cộng có 3.813 bệnh nhân được đưa vào nghiên cứu này, trong đó 29% tiêm đủ liều vắc-xin HPV. Có sự khác biệt đáng kể về việc tiêm đủ liều vắc-xin giữa các phòng khám (p<0,001), trong đó phòng khám Med/Peds có tỷ lệ tiêm đủ liều cao nhất (32%). Sau khi điều chỉnh các biến gây nhiễu, phân tích đa biến cho thấy Med/Peds và Y học gia đình có tỷ lệ tiêm đủ liều vắc-xin HPV cao hơn phòng khám Nhi khoa. Kết luận Chúng tôi thấy rằng khả năng tiêm đủ liều vắc-xin cao nhất khi bắt đầu tiêm ở các phòng khám Med/Peds và Y học gia đình, so với Nhi khoa và Y học vị thành niên. Những kết quả này cho thấy cần tiến hành thêm nghiên cứu định tính để hiểu chiến lược truyền thông nào hiệu quả nhất trong việc tạo điều kiện tiêm vắc-xin HPV ở thanh thiếu niên và làm thế nào để có thể chia sẻ các chiến lược hiệu quả nhất giữa các phòng khám."} +{"idx": 1870881, "en": "Monolayer C2N has been successfully synthesized. To explore the enhancement of its stability and the expansion of its potential applications in electronics and optoelectronics, a vertical van der Waals heterostructure is constructed by C2N monolayer integrated with h-BN monolayer. The first-principles calculations based on the density functional theory show that this structure possesses a type-II band alignment with a smaller direct band gap and lager band offsets, suggesting that photo-generated electron-hole pairs can be well spatially separated, and thus an excellent photoelectric and photovoltaic material can be obtained. Also shown is that the electronic properties of such a heterostructure can be effectively regulated by a vertical strain and external electric field. For example, under compressive strain or forward electric field, its band gap can be significantly reduced to enhance light-excitation electron transition further, meanwhile the feature of direct band-gap and large band offsets is always well-preserved. Furthermore, it is found that the intrinsic heterostructure holds a wide optic adsorption range and large adsorption coefficient, and the applied compressive strain or a positive electric field can lead to a wide and high main absorption peak across the near-infrared, visible light, and ultraviolet region, implying that a tuned heterostructure has more promising applications in optoelectronics.", "gemini": "Lớp đơn C2N đã được tổng hợp thành công. Để khám phá khả năng tăng cường độ ổn định và mở rộng các ứng dụng tiềm năng của nó trong điện tử và quang điện tử, một cấu trúc dị hướng van der Waals thẳng đứng được xây dựng bằng lớp đơn C2N tích hợp với lớp đơn h-BN. Các phép tính nguyên lý đầu tiên dựa trên lý thuyết hàm mật độ cho thấy cấu trúc này sở hữu sự liên kết băng loại II với khoảng cách băng trực tiếp nhỏ hơn và các dịch chuyển băng lớn hơn, cho thấy rằng các cặp lỗ trống electron sinh ra từ quang có thể được tách biệt tốt về mặt không gian, và do đó có thể thu được vật liệu quang điện và quang điện tuyệt vời. Cũng được chỉ ra là các tính chất điện tử của một cấu trúc dị hướng như vậy có thể được điều chỉnh hiệu quả bằng ứng suất thẳng đứng và điện trường ngoài. Ví dụ, dưới ứng suất nén hoặc điện trường thuận, khoảng cách băng của nó có thể giảm đáng kể để tăng cường hơn nữa quá trình chuyển đổi electron kích thích ánh sáng, trong khi đó đặc điểm của khoảng cách băng trực tiếp và các dịch chuyển băng lớn luôn được bảo toàn tốt. Hơn nữa, người ta thấy rằng cấu trúc dị hướng nội tại có dải hấp phụ quang học rộng và hệ số hấp phụ lớn, và ứng suất nén hoặc điện trường dương có thể dẫn đến đỉnh hấp thụ chính rộng và cao trên vùng cận hồng ngoại, ánh sáng khả kiến và vùng cực tím, ngụ ý rằng một cấu trúc dị hướng được điều chỉnh có nhiều ứng dụng hứa hẹn hơn trong quang điện tử."} +{"idx": 1870882, "en": "Acoustic metamaterials are artificial materials which can manipulate and control acoustic waves in way that may not exist in nature. Pentamode metamaterials, as one kind of metamaterials, have solid structures but behaves like fluid. One application is in building acoustic cloaks. In this paper, composite pentamode metamaterials with hexagonal unit cells are proposed. The phononic band structures of the unit cell show that there are band gaps within which only compressional modes exist. With variation of structures, highly anisotropic properties can be obtained. The influences of geometric dimensions and materials on the effective properties are analyzed. The composite structures introduce more degrees-of-freedom to tailor the effective properties.", "gemini": "Vật liệu siêu âm là vật liệu nhân tạo có thể thao túng và kiểm soát sóng âm theo cách mà có thể không tồn tại trong tự nhiên. Vật liệu siêu âm Pentamode, là một loại vật liệu siêu âm, có cấu trúc rắn nhưng hoạt động giống như chất lỏng. Một ứng dụng là chế tạo áo choàng âm thanh. Trong bài báo này, vật liệu siêu âm pentamode tổng hợp với các ô đơn vị hình lục giác được đề xuất. Cấu trúc dải phonon của ô đơn vị cho thấy có các khoảng trống dải mà chỉ tồn tại các chế độ nén. Với sự thay đổi của cấu trúc, có thể thu được các đặc tính dị hướng cao. Các ảnh hưởng của kích thước hình học và vật liệu đến các đặc tính hiệu quả được phân tích. Các cấu trúc tổng hợp đưa ra nhiều bậc tự do hơn để điều chỉnh các đặc tính hiệu quả."} +{"idx": 1870883, "en": "We report on the growth of high-quality stoichiometric layered Cr2Se3 single crystals with metallic and noncollinear antiferromagnetic ground state using the chemical vapor transport (CVT) method. The crystals show weak ferromagnetism in the in-plane and out-of-plane directions below the Neél temperature (T N), however, the field-cooled out-of-plane magnetization at 500 Oe and 10 K (∼0.24 μ B/f.u.) is approximately 15 times larger than that of the in-plane one, indicating strong c-axis easy uniaxial magnetic anisotropy, which is further supported by the in-plane and out-of-plane isothermal anisotropic magnetic hysteresis loops and the angular dependent magnetoresistance (MR). The latter also reveals a decrease of the coercive field of the crystal upon the tilting of the weak ferromagnetic easy axis away from the direction of the magnetic field. Further, the out-of-plane isothermal MR are negative below T N and show butterfly shapes for T < 10 K and couple with the magnetic hysteresis M(H) loop. These results may help researchers better understand the interplay between the weak ferromagnetism and the magnetotransport properties of 2D itinerant noncollinear antiferromagnetic systems.", "gemini": "Chúng tôi báo cáo về sự phát triển của các tinh thể đơn Cr2Se3 nhiều lớp có tỷ lượng thành phần cao chất lượng cao với trạng thái cơ bản là sắt từ kim loại và phản sắt từ không cộng tuyến bằng phương pháp vận chuyển hơi hóa học (CVT). Các tinh thể cho thấy tính sắt từ yếu theo hướng trong mặt phẳng và ngoài mặt phẳng dưới nhiệt độ Neél (T N), tuy nhiên, độ từ hóa ngoài mặt phẳng làm lạnh bằng từ trường ở 500 Oe và 10 K (∼0,24 μ B/f.u.) lớn hơn độ từ hóa trong mặt phẳng khoảng 15 lần, cho thấy tính dị hướng từ đơn trục dễ theo trục c mạnh, được hỗ trợ thêm bởi các vòng trễ từ đẳng hướng nhiệt độ trong mặt phẳng và ngoài mặt phẳng và điện trở từ phụ thuộc góc (MR). Về sau cũng cho thấy sự giảm của từ trường cưỡng bức của tinh thể khi trục dễ từ sắt yếu bị nghiêng ra khỏi hướng của từ trường. Hơn nữa, MR đẳng nhiệt ngoài mặt phẳng là âm dưới T N và cho thấy hình dạng cánh bướm đối với T < 10 K và kết hợp với vòng trễ từ M(H). Những kết quả này có thể giúp các nhà nghiên cứu hiểu rõ hơn về sự tương tác giữa tính sắt từ yếu và các tính chất vận chuyển từ của hệ thống phản sắt từ không cộng tuyến lang thang 2D."} +{"idx": 1870884, "en": "The fundamental problem of domain wall (DW) inertia-the property that gives to inertial behaviors remains unclear in the physics of magnetic solitons. To understand its nature as well as to achieve accurate DW positioning and efficient manipulation of domain wall motion (DWM), spin wave (SW) pulse-induced DW transient effect is studied both numerically and theoretically in a magnetic nanostrip. It is shown for the first time that there occurs inevitable deceleration/automotion after SW pulse, which indicates nonzero DW inertia. The induced DWM is revealed to relate to two factors: energy storing within DW and out-of-plane tilting of DW. To explain the DWM dynamics, a one-dimensional collective model is developed to account for the excitation of spin wave pulse. The model successfully bridges DW energy, DW tilting and DW displacement and provides descriptions in accordance with numerical findings. It is made clear that the DW automotion hence DW inertia originate from the process of DW relaxation toward equilibrium. The DW inertia is expressed in terms of effective mass and turns out to be a time-dependent function with damping constant α as the governing parameter, which opposes the nature of intrinsic mass. For case containing multiple DWs, the total effective mass is shown to concern the reached velocity and stored energy of DWs instead of the number of DWs, which is against common intuition.", "gemini": "Vấn đề cơ bản về quán tính của vách đômen (DW) - tính chất tạo ra các hành vi quán tính vẫn chưa rõ ràng trong vật lý soliton từ. Để hiểu bản chất của nó cũng như đạt được định vị DW chính xác và thao tác hiệu quả chuyển động của vách đômen (DWM), hiệu ứng tạm thời của DW do xung sóng spin (SW) được nghiên cứu cả về mặt số và lý thuyết trong một nanodải từ. Lần đầu tiên cho thấy rằng có sự giảm tốc/chuyển động tự động không thể tránh khỏi sau xung SW, biểu thị quán tính DW khác không. DWM được tạo ra được tiết lộ liên quan đến hai yếu tố: năng lượng lưu trữ trong DW và độ nghiêng ngoài mặt phẳng của DW. Để giải thích động lực học DWM, một mô hình tập thể một chiều được phát triển để giải thích cho sự kích thích của xung sóng spin. Mô hình này kết nối thành công năng lượng DW, độ nghiêng DW và độ dịch chuyển DW và cung cấp các mô tả phù hợp với các phát hiện số. Rõ ràng là chuyển động tự động của DW do đó quán tính DW bắt nguồn từ quá trình thư giãn DW hướng tới trạng thái cân bằng. Quán tính DW được biểu thị theo khối lượng hiệu dụng và hóa ra là một hàm phụ thuộc thời gian với hằng số giảm chấn α là tham số điều khiển, trái ngược với bản chất của khối lượng nội tại. Đối với trường hợp chứa nhiều DW, khối lượng hiệu dụng tổng thể được biểu thị liên quan đến vận tốc đạt được và năng lượng được lưu trữ của DW thay vì số lượng DW, trái với trực giác thông thường."} +{"idx": 1870885, "en": "Trapping and manipulating micro-size particles using optical tweezers has contributed to many breakthroughs in biology, materials science, and colloidal physics. However, it remains challenging to extend this technique to a few nanometers particles owing to the diffraction limit and the considerable Brownian motion of trapped nanoparticles. In this work, a nanometric optical tweezer is proposed by using a plasmonic nanocavity composed of the closely spaced silver coated fiber tip and gold film. It is found that the radial vector mode can produce a nano-sized near field with the electric-field intensity enhancement factor over 103 through exciting the plasmon gap mode in the nanocavity. By employing the Maxwell stress tensor formalism, we theoretically demonstrate that this nano-sized near field results in a sharp quasi-harmonic potential well, capable of stably trapping 2 nm quantum dots beneath the tip apex with the laser power as low as 3.7 mW. Further analysis reveals that our nanotweezers can stably work in a wide range of particle-to-tip distances, gap sizes, and operation wavelengths. We envision that our proposed nanometric optical tweezers could be compatible with the tip-enhanced Raman spectroscopy to allow simultaneously manipulating and characterizing single nanoparticles as well as nanoparticle interactions with high sensitivity.", "gemini": "Việc bẫy và thao tác các hạt có kích thước micro bằng nhíp quang đã góp phần vào nhiều đột phá trong sinh học, khoa học vật liệu và vật lý keo. Tuy nhiên, vẫn còn thách thức khi mở rộng kỹ thuật này cho các hạt có kích thước vài nanomet do giới hạn nhiễu xạ và chuyển động Brown đáng kể của các hạt nano bị kẹt. Trong nghiên cứu này, một nhíp quang kích thước nano được đề xuất bằng cách sử dụng một khoang nano plasmonic bao gồm đầu sợi phủ bạc và màng vàng cách nhau gần. Người ta thấy rằng chế độ vectơ xuyên tâm có thể tạo ra trường gần có kích thước nano với hệ số tăng cường cường độ trường điện trên 103 thông qua chế độ khe hở plasmon trong khoang nano. Bằng cách sử dụng công thức tenxơ ứng suất Maxwell, chúng tôi chứng minh về mặt lý thuyết rằng trường gần có kích thước nano này tạo ra một giếng thế bán hài hòa sắc nét, có khả năng giữ ổn định các chấm lượng tử 2 nm bên dưới đỉnh đầu nhọn với công suất laser thấp tới 3,7 mW. Phân tích sâu hơn cho thấy nhíp nano của chúng tôi có thể hoạt động ổn định trong một phạm vi rộng các khoảng cách hạt đến đầu nhọn, kích thước khe hở và bước sóng hoạt động. Chúng tôi hình dung rằng nhíp quang kích thước nano mà chúng tôi đề xuất có thể tương thích với phổ Raman tăng cường đầu nhọn để cho phép thao tác và đặc tính đồng thời các hạt nano đơn lẻ cũng như tương tác hạt nano với độ nhạy cao."} +{"idx": 1870886, "en": "In aging mice, osteoclast number increases in cortical bone but declines in trabecular bone, suggesting that different mechanisms underlie age-associated bone loss in these 2 compartments. Osteocytes produce the osteoclastogenic cytokine RANKL, encoded by Tnfsf11. Tnfsf11 mRNA increases in cortical bone of aged mice, suggesting a mechanism underlying the bone loss. To address this possibility, we aged mice lacking RANKL in osteocytes. Whereas control mice lost cortical bone between 8 and 24 months of age, mice lacking RANKL in osteocytes gained cortical bone during this period. Mice of both genotypes lost trabecular bone with age. Osteoclasts increased with age in cortical bone of control mice but not in RANKL conditional knockout mice. Induction of cellular senescence increased RANKL production in murine and human cell culture models, suggesting an explanation for elevated RANKL levels with age. Overexpression of the senescence-associated transcription factor Gata4 stimulated Tnfsf11 expression in cultured murine osteoblastic cells. Finally, elimination of senescent cells from aged mice using senolytic compounds reduced Tnfsf11 mRNA in cortical bone. Our results demonstrate the requirement of osteocyte-derived RANKL for age-associated cortical bone loss and suggest that increased Tnfsf11 expression with age results from accumulation of senescent cells in cortical bone.", "gemini": "Ở chuột già, số lượng tế bào hủy xương tăng ở xương vỏ nhưng giảm ở xương xốp, cho thấy các cơ chế khác nhau nằm dưới sự mất xương liên quan đến tuổi tác ở 2 ngăn này. Tế bào xương tạo ra cytokine sinh hủy xương RANKL, được mã hóa bởi Tnfsf11. mRNA Tnfsf11 tăng ở xương vỏ của chuột già, cho thấy một cơ chế nằm dưới sự mất xương. Để giải quyết khả năng này, chúng tôi đã nuôi những con chuột già không có RANKL trong tế bào xương. Trong khi những con chuột đối chứng mất xương vỏ trong độ tuổi từ 8 đến 24 tháng, những con chuột không có RANKL trong tế bào xương lại tăng xương vỏ trong giai đoạn này. Chuột của cả hai kiểu gen đều mất xương xốp theo tuổi tác. Tế bào hủy xương tăng theo tuổi tác ở xương vỏ của chuột đối chứng nhưng không ở chuột loại trừ có điều kiện RANKL. Việc gây ra tình trạng lão hóa tế bào làm tăng sản xuất RANKL trong các mô hình nuôi cấy tế bào của chuột và người, cho thấy một lời giải thích cho mức RANKL tăng cao theo tuổi tác. Sự biểu hiện quá mức của yếu tố phiên mã liên quan đến lão hóa Gata4 kích thích biểu hiện Tnfsf11 trong các tế bào tạo xương của chuột được nuôi cấy. Cuối cùng, việc loại bỏ các tế bào già khỏi chuột già bằng các hợp chất senolytic làm giảm mRNA Tnfsf11 ở xương vỏ. Kết quả của chúng tôi chứng minh yêu cầu của RANKL có nguồn gốc từ tế bào xương đối với mất xương vỏ liên quan đến tuổi tác và cho thấy rằng biểu hiện Tnfsf11 tăng theo tuổi tác là kết quả của sự tích tụ các tế bào già trong xương vỏ."} +{"idx": 1870887, "en": "Epithelial cell dysfunction has emerged as a central component of the pathophysiology of diffuse parenchymal diseases including idiopathic pulmonary fibrosis (IPF). Alveolar type 2 (AT2) cells represent a metabolically active lung cell population important for surfactant biosynthesis and alveolar homeostasis. AT2 cells and other distal lung epithelia, like all eukaryotic cells, contain an elegant quality control network to respond to intrinsic metabolic and biosynthetic challenges imparted by mutant protein conformers, dysfunctional subcellular organelles, and dysregulated telomeres. Failed AT2 quality control components (the ubiquitin-proteasome system, unfolded protein response, macroautophagy, mitophagy, and telomere maintenance) result in diverse cellular endophenotypes and molecular signatures including ER stress, defective autophagy, mitochondrial dysfunction, apoptosis, inflammatory cell recruitment, profibrotic signaling, and altered progenitor function that ultimately converge to drive downstream fibrotic remodeling in the IPF lung. As this complex network becomes increasingly better understood, opportunities will emerge to identify targets and therapeutic strategies for IPF.", "gemini": "Rối loạn chức năng tế bào biểu mô đã nổi lên như một thành phần trung tâm của bệnh sinh lý của các bệnh nhu mô lan tỏa bao gồm xơ phổi vô căn (IPF). Các tế bào phế nang loại 2 (AT2) đại diện cho một quần thể tế bào phổi hoạt động về mặt trao đổi chất quan trọng đối với quá trình sinh tổng hợp chất hoạt động bề mặt và cân bằng phế nang. Các tế bào AT2 và các biểu mô phổi xa khác, giống như tất cả các tế bào nhân chuẩn, chứa một mạng lưới kiểm soát chất lượng tuyệt vời để đáp ứng với các thách thức về trao đổi chất nội tại và sinh tổng hợp do các cấu tử protein đột biến, các bào quan dưới tế bào rối loạn chức năng và các telomere không được điều hòa gây ra. Các thành phần kiểm soát chất lượng AT2 bị lỗi (hệ thống ubiquitin-proteasome, phản ứng protein chưa gấp, đại thực bào, thực bào ty thể và duy trì telomere) dẫn đến các kiểu hình cuối tế bào và các đặc điểm phân tử đa dạng bao gồm căng thẳng ER, autophagy khiếm khuyết, rối loạn chức năng ty thể, apoptosis, tuyển dụng tế bào viêm, tín hiệu thúc đẩy xơ hóa và chức năng tiền thân bị thay đổi, cuối cùng hội tụ để thúc đẩy quá trình tái tạo xơ hóa hạ lưu ở phổi IPF. Khi mạng lưới phức tạp này ngày càng được hiểu rõ hơn, các cơ hội sẽ xuất hiện để xác định các mục tiêu và chiến lược điều trị cho IPF."} +{"idx": 1870888, "en": "Hypoxia-inducible factors (HIFs) and the HIF-dependent cancer hallmarks angiogenesis and metabolic rewiring are well-established drivers of breast cancer aggressiveness, therapy resistance, and poor prognosis. Targeting of HIF and its downstream targets in angiogenesis and metabolism has been unsuccessful so far in the breast cancer clinical setting, with major unresolved challenges residing in target selection, development of robust biomarkers for response prediction, and understanding and harnessing of escape mechanisms. This Review discusses the pathophysiological role of HIFs, angiogenesis, and metabolism in breast cancer and the challenges of targeting these features in patients with breast cancer. Rational therapeutic combinations, especially with immunotherapy and endocrine therapy, seem most promising in the clinical exploitation of the intricate interplay of HIFs, angiogenesis, and metabolism in breast cancer cells and the tumor microenvironment.", "gemini": "Các yếu tố có thể gây thiếu oxy (HIF) và các dấu hiệu đặc trưng của ung thư phụ thuộc HIF là sự hình thành mạch máu và sự tái cấu trúc chuyển hóa là những yếu tố thúc đẩy sự hung hăng của ung thư vú, tình trạng kháng trị và tiên lượng kém. Việc nhắm mục tiêu vào HIF và các mục tiêu hạ nguồn của nó trong quá trình hình thành mạch máu và chuyển hóa cho đến nay vẫn chưa thành công trong bối cảnh lâm sàng ung thư vú, với những thách thức lớn chưa được giải quyết nằm ở việc lựa chọn mục tiêu, phát triển các dấu ấn sinh học mạnh mẽ để dự đoán đáp ứng và hiểu biết cũng như khai thác các cơ chế thoát. Bài tổng quan này thảo luận về vai trò bệnh sinh của HIF, quá trình hình thành mạch máu và chuyển hóa trong ung thư vú và những thách thức trong việc nhắm mục tiêu vào các đặc điểm này ở những bệnh nhân ung thư vú. Các phối hợp điều trị hợp lý, đặc biệt là với liệu pháp miễn dịch và liệu pháp nội tiết, có vẻ hứa hẹn nhất trong việc khai thác lâm sàng sự tương tác phức tạp của HIF, quá trình hình thành mạch máu và chuyển hóa trong các tế bào ung thư vú và vi môi trường khối u."} +{"idx": 1870889, "en": "Lupus nephritis (LN) is a major organ complication and cause of morbidity and mortality in patients with systemic lupus erythematosus (SLE). There is an unmet medical need for developing more efficient and specific, mechanism-based therapies, which depends on improved understanding of the underlying LN pathogenesis. Here we present direct visual evidence from high-power intravital imaging of the local kidney tissue microenvironment in mouse models showing that activated memory T cells originated in immune organs and the LN-specific robust accumulation of the glomerular endothelial glycocalyx played central roles in LN development. The glomerular homing of T cells was mediated via the direct binding of their CD44 to the hyaluronic acid (HA) component of the endothelial glycocalyx, and glycocalyx-degrading enzymes efficiently disrupted homing. Short-course treatment with either hyaluronidase or heparinase III provided long-term organ protection as evidenced by vastly improved albuminuria and survival rate. This glycocalyx/HA/memory T cell interaction is present in multiple SLE-affected organs and may be therapeutically targeted for SLE complications, including LN.", "gemini": "Viêm thận lupus (LN) là một biến chứng ở cơ quan chính và là nguyên nhân gây bệnh tật và tử vong ở những bệnh nhân mắc bệnh lupus ban đỏ hệ thống (SLE). Có một nhu cầu y tế chưa được đáp ứng là phát triển các liệu pháp dựa trên cơ chế, hiệu quả và đặc hiệu hơn, điều này phụ thuộc vào việc hiểu biết sâu hơn về cơ chế sinh bệnh LN tiềm ẩn. Ở đây, chúng tôi trình bày bằng chứng trực quan trực tiếp từ hình ảnh nội soi trong ống kính có độ phóng đại cao về vi môi trường mô thận tại chỗ ở các mô hình chuột cho thấy các tế bào T nhớ hoạt hóa có nguồn gốc từ các cơ quan miễn dịch và sự tích tụ mạnh mẽ của glycocalyx nội mô cầu thận đặc hiệu LN đóng vai trò trung tâm trong sự phát triển của LN. Sự di chuyển về cầu thận của các tế bào T được trung gian thông qua sự liên kết trực tiếp của CD44 với thành phần axit hyaluronic (HA) của glycocalyx nội mô, và các enzym phân hủy glycocalyx làm gián đoạn hiệu quả sự di chuyển về cầu thận. Liệu trình điều trị ngắn hạn bằng hyaluronidase hoặc heparinase III cung cấp khả năng bảo vệ cơ quan lâu dài bằng chứng là tình trạng albumin niệu và tỷ lệ sống sót được cải thiện rất nhiều. Tương tác glycocalyx/HA/tế bào T nhớ này có mặt ở nhiều cơ quan bị SLE ảnh hưởng và có thể được nhắm mục tiêu điều trị cho các biến chứng của SLE, bao gồm LN."} +{"idx": 1870890, "en": "Hypoxia/HIF-1α- and extracellular adenosine/A2 adenosine receptor-mediated immunosuppression protects tissues from collateral damage by antipathogen immune cells. However, this mechanism also protects cancerous tissues by inhibiting antitumor immune cells in hypoxic and extracellular adenosine-rich tumors that are the most resistant to current therapies. Here, we explain a potentially novel, antiimmunosuppressive reasoning to justify strategies using respiratory hyperoxia and oxygenation agents in cancer treatment. Earlier attempts to use oxygenation of tumors as a monotherapy or to improve radiotherapy have failed because oxygenation protocols were not combined with immunotherapies of cancer. In contrast, the proposal for therapeutic use of antihypoxic oxygenation described here was motivated by the need to prevent the hypoxia/HIF-1α-driven accumulation of extracellular adenosine to (a) unleash antitumor immune cells from inhibition by intracellular cAMP and (b) prevent immunosuppressive transcription of cAMP response element- and hypoxia response element-containing immunosuppressive gene products (e.g., TGF-β). Use of oxygenation agents together with inhibitors of the A2A adenosine receptor may be required to enable the most effective cancer immunotherapy. The emerging outcomes of clinical trials of cancer patients refractory to all other treatments provide support for the molecular and immunological mechanism-based approach to cancer immunotherapy described here.", "gemini": "Ức chế miễn dịch do thiếu oxy/HIF-1α và adenosine ngoại bào/ thụ thể adenosine A2 bảo vệ các mô khỏi tổn thương do các tế bào miễn dịch chống lại mầm bệnh. Tuy nhiên, cơ chế này cũng bảo vệ các mô ung thư bằng cách ức chế các tế bào miễn dịch chống ung thư trong các khối u thiếu oxy và giàu adenosine ngoại bào, đây là những khối u có khả năng kháng nhất với các liệu pháp hiện tại. Ở đây, chúng tôi giải thích một lý luận ức chế miễn dịch mới, có khả năng chống lại để biện minh cho các chiến lược sử dụng các tác nhân tăng oxy hô hấp và oxy hóa trong điều trị ung thư. Những nỗ lực trước đó nhằm sử dụng oxy hóa khối u như một liệu pháp đơn trị hoặc để cải thiện xạ trị đã thất bại vì các phác đồ oxy hóa không được kết hợp với liệu pháp miễn dịch ung thư. Ngược lại, đề xuất sử dụng oxy hóa chống thiếu oxy để điều trị được mô tả ở đây được thúc đẩy bởi nhu cầu ngăn ngừa sự tích tụ adenosine ngoại bào do thiếu oxy/HIF-1α để (a) giải phóng các tế bào miễn dịch chống ung thư khỏi sự ức chế của cAMP nội bào và (b) ngăn chặn phiên mã ức chế miễn dịch của sản phẩm gen ức chế miễn dịch có chứa yếu tố đáp ứng cAMP và yếu tố đáp ứng thiếu oxy (ví dụ: TGF-β). Có thể cần sử dụng các tác nhân oxy hóa cùng với các chất ức chế thụ thể adenosine A2A để có thể thực hiện liệu pháp miễn dịch ung thư hiệu quả nhất. Các kết quả mới nổi từ các thử nghiệm lâm sàng trên bệnh nhân ung thư không đáp ứng với tất cả các phương pháp điều trị khác cung cấp hỗ trợ cho phương pháp tiếp cận liệu pháp miễn dịch ung thư dựa trên cơ chế phân tử và miễn dịch được mô tả ở đây."} +{"idx": 1870891, "en": "Most of the patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mount a humoral immune response to the virus within a few weeks of infection, but the duration of this response and how it correlates with clinical outcomes has not been completely characterized. Of particular importance is the identification of immune correlates of infection that would support public health decision-making on treatment approaches, vaccination strategies, and convalescent plasma therapy. While ELISA-based assays to detect and quantitate antibodies to SARS-CoV-2 in patient samples have been developed, the detection of neutralizing antibodies typically requires more demanding cell-based viral assays. Here, we present a safe and efficient protein-based assay for the detection of serum and plasma antibodies that block the interaction of the SARS-CoV-2 spike protein receptor binding domain (RBD) with its receptor, angiotensin-converting enzyme 2 (ACE2). The assay serves as a surrogate neutralization assay and is performed on the same platform and in parallel with an ELISA for the detection of antibodies against the RBD, enabling a direct comparison. The results obtained with our assay correlate with those of 2 viral-based assays, a plaque reduction neutralization test (PRNT) that uses live SARS-CoV-2 virus and a spike pseudotyped viral vector-based assay.", "gemini": "Hầu hết các bệnh nhân bị nhiễm virus corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2) đều có phản ứng miễn dịch dịch thể với virus trong vòng vài tuần kể từ khi nhiễm bệnh, nhưng thời gian của phản ứng này và cách thức tương quan với kết quả lâm sàng vẫn chưa được mô tả đầy đủ. Điều đặc biệt quan trọng là việc xác định các tương quan miễn dịch của nhiễm trùng có thể hỗ trợ cho việc ra quyết định của y tế công cộng về các phương pháp điều trị, chiến lược tiêm chủng và liệu pháp huyết tương hồi phục. Trong khi các xét nghiệm dựa trên ELISA để phát hiện và định lượng kháng thể đối với SARS-CoV-2 trong các mẫu bệnh phẩm đã được phát triển, thì việc phát hiện kháng thể trung hòa thường đòi hỏi các xét nghiệm vi-rút dựa trên tế bào khắt khe hơn. Tại đây, chúng tôi trình bày một xét nghiệm dựa trên protein an toàn và hiệu quả để phát hiện các kháng thể trong huyết thanh và huyết tương ngăn chặn sự tương tác của miền liên kết thụ thể protein gai SARS-CoV-2 (RBD) với thụ thể của nó, enzyme chuyển đổi angiotensin 2 (ACE2). Xét nghiệm này đóng vai trò như một xét nghiệm trung hòa thay thế và được thực hiện trên cùng một nền tảng và song song với xét nghiệm ELISA để phát hiện kháng thể chống lại RBD, cho phép so sánh trực tiếp. Các kết quả thu được bằng xét nghiệm của chúng tôi tương quan với kết quả của 2 xét nghiệm dựa trên vi-rút, xét nghiệm trung hòa giảm mảng bám (PRNT) sử dụng vi-rút SARS-CoV-2 sống và xét nghiệm dựa trên vectơ vi-rút giả tuýp gai."} +{"idx": 1870892, "en": "We report spatiotemporal pure-rotational coherent anti-Stokes Raman spectroscopy (CARS) in a one-dimensional imaging arrangement obtained with a single ultrafast regenerative amplifier system. The femtosecond pump/Stokes photon pairs, used for impulsive excitation, are delivered by an external compressor operating on a ∼35% beam split of the uncompressed amplifier output (2.5 mJ/pulse). The picosecond 1.2 mJ probe pulse is produced via the second-harmonic bandwidth compression (SHBC) of the ∼65% remainder of the amplifier output (4.5 mJ/pulse), which originates from the internal compressor. The two pump/Stokes and probe pulses are spatially, temporally, and repetition-wise correlated at the measurement, and the signal generation plane is relayed by a wide-field coherent imaging spectrometer onto the detector plane, which is refreshed at the same repetition rate as the ultrafast regenerative amplifier system. We demonstrate 1 kHz cinematographic 1D-CARS gas-phase thermometry across an unstable premixed methane/air flame-front, achieved with a single-shot precision <1% and accuracy <3%, 1.4 mm field of view, and an excellent <20µm line-spread function.", "gemini": "Chúng tôi báo cáo quang phổ Raman phản Stokes mạch lạc quay tinh khiết theo không gian thời gian (CARS) trong một sắp xếp hình ảnh một chiều thu được bằng một hệ thống khuếch đại tái sinh siêu nhanh duy nhất. Các cặp photon bơm/Stokes femto giây, được sử dụng để kích thích xung, được cung cấp bởi một bộ nén bên ngoài hoạt động trên chùm tia tách ra ∼35% của đầu ra bộ khuếch đại chưa nén (2,5 mJ/xung). Xung thăm dò pico giây 1,2 mJ được tạo ra thông qua nén băng thông sóng hài thứ hai (SHBC) của phần còn lại ∼65% của đầu ra bộ khuếch đại (4,5 mJ/xung), có nguồn gốc từ bộ nén bên trong. Hai xung bơm/Stokes và xung thăm dò được tương quan về mặt không gian, thời gian và lặp lại tại phép đo và mặt phẳng tạo tín hiệu được chuyển tiếp bởi máy quang phổ hình ảnh mạch lạc trường rộng lên mặt phẳng đầu dò, được làm mới ở cùng tốc độ lặp lại như hệ thống khuếch đại tái sinh siêu nhanh. Chúng tôi trình bày nhiệt kế pha khí 1D-CARS điện ảnh 1 kHz trên mặt trận ngọn lửa hỗn hợp trước không ổn định metan/không khí, đạt được với độ chính xác một lần chụp <1% và độ chính xác <3%, trường nhìn 1,4 mm và hàm trải rộng đường tuyệt vời <20µm."} +{"idx": 1870893, "en": "This study considers the reflective characteristics of three-component Si/Al/Sc multilayer mirrors with a MoSi2 protective cap layer as candidates for telescopes for observation of the solar corona in the He I (λ=58.4nm) spectral line. At 58.4 nm, a peak reflectance of 32% and a spectral width at a half-maximum intensity of Δλ=5.4nm are obtained. The temporal stability of the reflectance at λ=58.4nm for Si/Al/Sc samples with a 6 nm thick MoSi2 cap layer is investigated during storage in air for 20 months.", "gemini": "Nghiên cứu này xem xét các đặc tính phản xạ của gương nhiều lớp Si/Al/Sc ba thành phần có lớp phủ bảo vệ MoSi2 như ứng cử viên cho kính viễn vọng để quan sát vành nhật hoa trong vạch quang phổ He I (λ=58,4nm). Ở bước sóng 58,4 nm, độ phản xạ cực đại đạt 32% và độ rộng quang phổ ở cường độ cực đại bằng một nửa là Δλ=5,4nm. Độ ổn định theo thời gian của độ phản xạ ở λ=58,4nm đối với các mẫu Si/Al/Sc có lớp phủ MoSi2 dày 6 nm được nghiên cứu trong quá trình bảo quản trong không khí trong 20 tháng."} +{"idx": 1870894, "en": "Despite advances in identifying the key immunoregulatory roles of many of the human leukocyte immunoglobulin-like receptor (LILR) family members, the function of the inhibitory molecule LILRB3 (ILT5, CD85a, LIR3) remains unclear. Studies indicate a predominant myeloid expression; however, high homology within the LILR family and a relative paucity of reagents have hindered progress toward identifying the function of this receptor. To investigate its function and potential immunomodulatory capacity, a panel of LILRB3-specific monoclonal antibodies (mAbs) was generated. LILRB3-specific mAbs bound to discrete epitopes in Ig-like domain 2 or 4. LILRB3 ligation on primary human monocytes by an agonistic mAb resulted in phenotypic and functional changes, leading to potent inhibition of immune responses in vitro, including significant reduction in T cell proliferation. Importantly, agonizing LILRB3 in humanized mice induced tolerance and permitted efficient engraftment of allogeneic cells. Our findings reveal powerful immunosuppressive functions of LILRB3 and identify it as an important myeloid checkpoint receptor.", "gemini": "Mặc dù có những tiến bộ trong việc xác định các vai trò điều hòa miễn dịch chính của nhiều thành viên trong họ thụ thể giống immunoglobulin của bạch cầu người (LILR), chức năng của phân tử ức chế LILRB3 (ILT5, CD85a, LIR3) vẫn chưa rõ ràng. Các nghiên cứu chỉ ra rằng biểu hiện chủ yếu ở dòng tủy; tuy nhiên, sự đồng đẳng cao trong họ LILR và sự thiếu hụt tương đối của các thuốc thử đã cản trở tiến trình xác định chức năng của thụ thể này. Để nghiên cứu chức năng và khả năng điều hòa miễn dịch tiềm tàng của nó, một nhóm các kháng thể đơn dòng (mAb) đặc hiệu LILRB3 đã được tạo ra. Các mAb đặc hiệu LILRB3 liên kết với các epitop riêng biệt trong miền giống Ig 2 hoặc 4. Sự gắn kết LILRB3 trên các tế bào đơn nhân người nguyên phát bằng mAb chủ vận dẫn đến những thay đổi về kiểu hình và chức năng, dẫn đến ức chế mạnh các phản ứng miễn dịch trong ống nghiệm, bao gồm cả việc giảm đáng kể sự tăng sinh của tế bào T. Quan trọng là việc kích hoạt LILRB3 ở những con chuột được nhân bản ở người đã gây ra tình trạng dung nạp và cho phép ghép tế bào đồng loại hiệu quả. Những phát hiện của chúng tôi cho thấy các chức năng ức chế miễn dịch mạnh mẽ của LILRB3 và xác định nó là một thụ thể điểm kiểm tra dòng tủy quan trọng."} +{"idx": 1870895, "en": "Cavity-free lasing of N2+ induced by a femtosecond laser pulse at 800 nm is nearly totally suppressed by a delayed twin control pulse. We explain this surprising effect within the V-scheme of lasing without population inversion. A fast transfer of population between nitrogen ionic states X2Σg+ and A2Πu, induced by the second pulse, terminates the conditions for amplification in the system. The appearance of short lasing bursts at delays corresponding to revivals of rotational wave packets is explained along the same lines.", "gemini": "Tia laser không có hốc của N2+ được tạo ra bởi xung laser femtosecond ở 800 nm gần như hoàn toàn bị ức chế bởi một xung điều khiển kép trễ. Chúng tôi giải thích hiệu ứng đáng ngạc nhiên này trong sơ đồ V của quá trình tạo tia laser mà không đảo ngược dân số. Sự chuyển dịch nhanh chóng của dân số giữa các trạng thái ion nitơ X2Σg+ và A2Πu, do xung thứ hai gây ra, chấm dứt các điều kiện khuếch đại trong hệ thống. Sự xuất hiện của các chùm tia laser ngắn ở độ trễ tương ứng với sự hồi sinh của các gói sóng quay được giải thích theo cùng một hướng."} +{"idx": 1870896, "en": "The Ca2+-binding protein calmodulin has emerged as a pivotal player in tuning Na+ channel function, although its impact in vivo remains to be resolved. Here, we identify the role of calmodulin and the NaV1.5 interactome in regulating late Na+ current in cardiomyocytes. We created transgenic mice with cardiac-specific expression of human NaV1.5 channels with alanine substitutions for the IQ motif (IQ/AA). The mutations rendered the channels incapable of binding calmodulin to the C-terminus. The IQ/AA transgenic mice exhibited normal ventricular repolarization without arrhythmias and an absence of increased late Na+ current. In comparison, transgenic mice expressing a lidocaine-resistant (F1759A) human NaV1.5 demonstrated increased late Na+ current and prolonged repolarization in cardiomyocytes, with spontaneous arrhythmias. To determine regulatory factors that prevent late Na+ current for the IQ/AA mutant channel, we considered fibroblast growth factor homologous factors (FHFs), which are within the NaV1.5 proteomic subdomain shown by proximity labeling in transgenic mice expressing NaV1.5 conjugated to ascorbate peroxidase. We found that FGF13 diminished late current of the IQ/AA but not F1759A mutant cardiomyocytes, suggesting that endogenous FHFs may serve to prevent late Na+ current in mouse cardiomyocytes. Leveraging endogenous mechanisms may furnish an alternative avenue for developing novel pharmacology that selectively blunts late Na+ current.", "gemini": "Protein liên kết Ca2+ calmodulin đã nổi lên như một yếu tố quan trọng trong việc điều chỉnh chức năng kênh Na+, mặc dù tác động của nó trong cơ thể sống vẫn chưa được giải quyết. Ở đây, chúng tôi xác định vai trò của calmodulin và tương tác NaV1.5 trong việc điều chỉnh dòng Na+ muộn ở tế bào cơ tim. Chúng tôi đã tạo ra những con chuột chuyển gen có biểu hiện đặc hiệu ở tim của các kênh NaV1.5 của người với sự thay thế alanine cho mô típ IQ (IQ/AA). Các đột biến khiến các kênh không có khả năng liên kết calmodulin với đầu C. Chuột chuyển gen IQ/AA biểu hiện sự tái phân cực thất bình thường mà không có loạn nhịp tim và không có dòng Na+ muộn tăng lên. Ngược lại, chuột chuyển gen biểu hiện NaV1.5 của người kháng lidocaine (F1759A) cho thấy dòng Na+ muộn tăng lên và tái phân cực kéo dài ở tế bào cơ tim, với các loạn nhịp tim tự phát. Để xác định các yếu tố điều hòa ngăn ngừa dòng Na+ muộn đối với kênh đột biến IQ/AA, chúng tôi đã xem xét các yếu tố đồng đẳng với yếu tố tăng trưởng nguyên bào sợi (FHF), nằm trong miền phụ proteomic NaV1.5 được thể hiện bằng cách gắn nhãn gần ở chuột chuyển gen biểu hiện NaV1.5 liên hợp với ascorbate peroxidase. Chúng tôi thấy rằng FGF13 làm giảm dòng muộn của tế bào cơ tim đột biến IQ/AA nhưng không phải F1759A, điều này cho thấy rằng FHF nội sinh có thể đóng vai trò ngăn ngừa dòng Na+ muộn ở tế bào cơ tim chuột. Việc tận dụng các cơ chế nội sinh có thể cung cấp một hướng đi mới để phát triển dược lý mới làm giảm dòng Na+ muộn một cách chọn lọc."} +{"idx": 1870897, "en": "It is of scientific significance to explore the terahertz radiation source with the performances of high power, tunable frequency, and controllable chirp for the realization of coherent control of quantum systems. How to realize frequency chirp control of terahertz synchrotron radiation is the last puzzle to be completed. In this Letter, we propose a method to control the radiation frequency chirp with precision. A novel photomixing scheme is presented to generate a longitudinally modulated laser pulse with non-uniform time intervals between the adjacent micro-peaks, which means that there is a chirp in the modulation frequency, and this chirp can be continuously tuned. The interaction is made to occur between an electron beam and the modulated laser pulse in a modulator (an undulator tuned at the laser wavelength), then terahertz synchrotron radiation with the same spectrum characteristics as the modulated laser will be generated when the electron beam passes through the following bending magnet. We expect that this method will open a new way for the coherent control of quantum systems in the terahertz regime.", "gemini": "Việc khám phá nguồn bức xạ terahertz với hiệu suất công suất cao, tần số có thể điều chỉnh và chirp có thể kiểm soát để hiện thực hóa sự kiểm soát mạch lạc của các hệ thống lượng tử có ý nghĩa khoa học. Làm thế nào để hiện thực hóa sự kiểm soát chirp tần số của bức xạ synchrotron terahertz là câu đố cuối cùng cần được hoàn thành. Trong Bức thư này, chúng tôi đề xuất một phương pháp để kiểm soát chirp tần số bức xạ một cách chính xác. Một chương trình trộn ảnh mới được trình bày để tạo ra một xung laser được điều chế theo chiều dọc với các khoảng thời gian không đồng đều giữa các đỉnh vi liền kề, điều đó có nghĩa là có một chirp trong tần số điều chế và chirp này có thể được điều chỉnh liên tục. Sự tương tác được thực hiện giữa chùm electron và xung laser được điều chế trong bộ điều chế (bộ phát sóng được điều chỉnh theo bước sóng laser), sau đó bức xạ synchrotron terahertz với các đặc tính quang phổ giống như laser được điều chế sẽ được tạo ra khi chùm electron đi qua nam châm uốn cong sau đây. Chúng tôi hy vọng rằng phương pháp này sẽ mở ra một cách mới để kiểm soát mạch lạc các hệ thống lượng tử trong chế độ terahertz."} +{"idx": 1870898, "en": "Optoacoustic interaction can be strongly enhanced in tiny core fibers, and it holds significant potential for stable harmonic mode-locking at gigahertz (GHz) and higher repetition rate. In this Letter, we propose and demonstrate a microfiber-assisted GHz harmonic mode-locking fiber laser, which is achieved by the enhanced optomechanical coupling between cavity modes in microfiber with the waist length of ∼16cm and the waist diameter of ∼1.56µm. The repetition rates can be stably locked at 2.3828 GHz and predominately locked at 1.7852 GHz, corresponding to the frequencies of radial R01 and torsional-radial TR21 acoustic modes, respectively. Our results provide novel insight into the design of a high-repetition-rate laser source and the application of microfibers in the optomechanical field.", "gemini": "Tương tác quang âm có thể được tăng cường mạnh mẽ trong các sợi lõi nhỏ và nó có tiềm năng đáng kể để khóa chế độ sóng hài ổn định ở tốc độ lặp lại gigahertz (GHz) và cao hơn. Trong Bức thư này, chúng tôi đề xuất và trình diễn một sợi laser khóa chế độ sóng hài GHz được hỗ trợ bằng sợi nhỏ, đạt được bằng cách ghép quang cơ được tăng cường giữa các chế độ khoang trong sợi nhỏ với chiều dài eo là ∼16cm và đường kính eo là ∼1,56µm. Tốc độ lặp lại có thể được khóa ổn định ở 2,3828 GHz và chủ yếu được khóa ở 1,7852 GHz, tương ứng với tần số của các chế độ âm thanh hướng tâm R01 và xoắn-hướng tâm TR21. Kết quả của chúng tôi cung cấp cái nhìn sâu sắc mới vào việc thiết kế nguồn laser tốc độ lặp lại cao và ứng dụng của sợi nhỏ trong lĩnh vực quang cơ."} +{"idx": 1870899, "en": "Noninvasive, objective measurement of rod function is as significant as that of cone function, and for retinal diseases such as retinitis pigmentosa and age-related macular degeneration, rod function may be a more sensitive biomarker of disease progression and efficacy of treatment than cone function. Functional imaging of single human rod photoreceptors, however, has proven difficult because their small size and rapid functional response pose challenges for the resolution and speed of the imaging system. Here, we describe light-evoked, functional responses of human rods and cones, measured noninvasively using a synchronized adaptive optics optical coherence tomography (OCT) and scanning light ophthalmoscopy (SLO) system. The higher lateral resolution of the SLO images made it possible to confirm the identity of rods in the corresponding OCT volumes.", "gemini": "Đo lường chức năng tế bào que không xâm lấn, khách quan cũng quan trọng như đo lường chức năng tế bào nón, và đối với các bệnh võng mạc như viêm võng mạc sắc tố và thoái hóa điểm vàng do tuổi tác, chức năng tế bào que có thể là dấu ấn sinh học nhạy hơn về tiến triển bệnh và hiệu quả điều trị so với chức năng tế bào nón. Tuy nhiên, việc chụp ảnh chức năng của các thụ thể ánh sáng tế bào que ở người đơn lẻ đã được chứng minh là rất khó khăn vì kích thước nhỏ và phản ứng chức năng nhanh của chúng đặt ra những thách thức đối với độ phân giải và tốc độ của hệ thống chụp ảnh. Tại đây, chúng tôi mô tả các phản ứng chức năng do ánh sáng gây ra của tế bào que và tế bào nón ở người, được đo một cách không xâm lấn bằng cách sử dụng hệ thống chụp cắt lớp quang học thích ứng đồng bộ (OCT) và soi đáy mắt quét (SLO). Độ phân giải bên cao hơn của hình ảnh SLO giúp xác nhận danh tính của các tế bào que trong các thể tích OCT tương ứng."} +{"idx": 1870900, "en": "We investigate the combination of a nonlinear frequency division multiplexed (NFDM) transmission scheme with midpoint optical phase conjugation (OPC), and show that midpoint OPC introduces power enhancement by compensating for nonlinear impairments. It offers a degree of freedom to have a flexible power normalization factor, Pn. Optimizing Pn helps minimize the signal-noise mixing in nonlinear Fourier transform processing for a specific launch power, resulting in improving the system performance significantly. The mid-OPC NFDM system can provide 4.5 dB and 5.6 dB advantages in Q-factor as compared to the conventional NFDM system when the transmission fiber is standard single-mode fiber and a fiber with optimum dispersion, respectively. Mid-OPC NFDM can also offer higher spectral efficiency at a longer transmission reach due to the shorter guard interval.", "gemini": "Chúng tôi nghiên cứu sự kết hợp của một sơ đồ truyền đa kênh phân chia tần số phi tuyến (NFDM) với liên hợp pha quang giữa điểm (OPC) và cho thấy rằng OPC giữa điểm tạo ra sự tăng cường công suất bằng cách bù đắp cho các suy giảm phi tuyến. Nó cung cấp một mức độ tự do để có hệ số chuẩn hóa công suất linh hoạt, Pn. Tối ưu hóa Pn giúp giảm thiểu nhiễu hỗn hợp tín hiệu trong xử lý biến đổi Fourier phi tuyến cho một công suất phát cụ thể, do đó cải thiện đáng kể hiệu suất của hệ thống. Hệ thống NFDM OPC giữa có thể cung cấp các lợi thế về hệ số Q là 4,5 dB và 5,6 dB so với hệ thống NFDM thông thường khi sợi truyền là sợi đơn mode chuẩn và sợi có độ phân tán tối ưu, tương ứng. NFDM OPC giữa cũng có thể cung cấp hiệu suất phổ cao hơn ở phạm vi truyền dài hơn do khoảng bảo vệ ngắn hơn."} +{"idx": 1870901, "en": "Despite significant merit of depth representation, holographic displays have a considerable limitation: speckle. Here, we present speckle reduced holographic displays using an engineered light source with angle diversity for speckle reduction. The level of angle diversity is optimized with consideration of resolution, speckle contrast, and depth of field. To extend the depth of field sacrificed for speckle reduction, we apply tomographic synthesis, exploiting synchronization of a local illumination module and a tunable-focus lens. We implement a benchtop prototype to verify the proposed method, which reduces the speckle contrast averagely by 37.8% while preserving resolution and 4.0 diopter depth of field.", "gemini": "Mặc dù có ưu điểm đáng kể về khả năng biểu diễn chiều sâu, nhưng màn hình hiển thị ba chiều có một hạn chế đáng kể: nhiễu hạt. Tại đây, chúng tôi trình bày màn hình hiển thị ba chiều giảm nhiễu hạt bằng cách sử dụng nguồn sáng được thiết kế với sự đa dạng về góc để giảm nhiễu hạt. Mức độ đa dạng về góc được tối ưu hóa khi xem xét độ phân giải, độ tương phản nhiễu hạt và độ sâu trường ảnh. Để mở rộng độ sâu trường ảnh bị hy sinh để giảm nhiễu hạt, chúng tôi áp dụng tổng hợp cắt lớp, khai thác sự đồng bộ của mô-đun chiếu sáng cục bộ và ống kính lấy nét có thể điều chỉnh. Chúng tôi triển khai một nguyên mẫu trên mặt bàn để xác minh phương pháp được đề xuất, giúp giảm độ tương phản nhiễu hạt trung bình 37,8% trong khi vẫn giữ nguyên độ phân giải và độ sâu trường ảnh 4,0 điốp."} +{"idx": 1870902, "en": "Ultra-short optical pulses in the ultraviolet (UV) region are of significant interest for combustion and reacting flow diagnostics, as most important chemical species have electronic resonance transitions in the UV region. Optical parametric amplifiers are typically used for frequency conversion of femtosecond (fs) pulses from near-IR to UV; however, their implementation for practical imaging applications is limited because of the low conversion efficiency and extreme sensitivity to ambient conditions. In this work, we report the implementation of direct-frequency-tripled, fs laser pulses from a tunable amplified laser system for high-resolution imaging of hydroxyl (OH) radical in flames. The fundamental laser output near 850 nm is frequency tripled to obtain approximately 283.3-nm UV radiation. OH planar laser-induced fluorescence (PLIF) imaging at 1 kHz is demonstrated in turbulent flames with image sheet heights in excess of 45 mm and a signal-to-noise ratio better than 25. These results represent over 3× increase in the imaging dimensionality compared to traditional OPA-based systems. Additionally, the third-harmonic generation apparatus is compact, robust, and easy to operate while providing near-Gaussian beam profiles. Simple power scaling suggests another factor of 3 or more increase in sheet height can be achieved for kilohertz-rate practical combustion diagnostics applications.", "gemini": "Các xung quang siêu ngắn trong vùng cực tím (UV) có ý nghĩa quan trọng đối với chẩn đoán quá trình cháy và dòng phản ứng, vì hầu hết các loài hóa học quan trọng đều có các chuyển đổi cộng hưởng điện tử trong vùng UV. Các bộ khuếch đại tham số quang thường được sử dụng để chuyển đổi tần số các xung femto giây (fs) từ gần IR sang UV; tuy nhiên, việc triển khai chúng cho các ứng dụng hình ảnh thực tế bị hạn chế do hiệu suất chuyển đổi thấp và độ nhạy cực cao đối với các điều kiện xung quanh. Trong công trình này, chúng tôi báo cáo việc triển khai các xung laser fs tần số ba trực tiếp từ hệ thống laser khuếch đại có thể điều chỉnh để chụp ảnh độ phân giải cao gốc hydroxyl (OH) trong ngọn lửa. Đầu ra laser cơ bản gần 850 nm có tần số tăng gấp ba để thu được bức xạ UV khoảng 283,3 nm. Đã chứng minh hình ảnh huỳnh quang kích thích bằng laser phẳng OH (PLIF) ở tần số 1 kHz trong ngọn lửa nhiễu động với chiều cao tấm ảnh vượt quá 45 mm và tỷ lệ tín hiệu trên nhiễu tốt hơn 25. Những kết quả này thể hiện sự gia tăng hơn 3 lần về kích thước hình ảnh so với các hệ thống dựa trên OPA truyền thống. Ngoài ra, thiết bị tạo sóng hài bậc ba nhỏ gọn, chắc chắn và dễ vận hành đồng thời cung cấp các cấu hình chùm gần Gauss. Tỷ lệ công suất đơn giản cho thấy có thể đạt được một hệ số khác tăng 3 lần trở lên chiều cao của tấm để ứng dụng chẩn đoán quá trình đốt cháy thực tế ở tốc độ kilohertz."} +{"idx": 1870903, "en": "We demonstrate a reflective wavefront sensor grating suitable for the characterization of high-quality x-ray beamlines and optical systems with high power densities. Operating at glancing incidence angles, the optical element is deeply etched with a two-level pattern of shearing interferometry gratings and Hartmann wavefront sensor grids. Transverse features block unwanted light, enabling binary amplitude in reflection with high pattern contrast. We present surface characterization and soft x-ray reflectometry of a prototype grating array to demonstrate function prior to wavefront measurement applications. A simulation of device performance is shown.", "gemini": "Chúng tôi trình bày một mạng cảm biến mặt sóng phản xạ phù hợp để mô tả các chùm tia X chất lượng cao và hệ thống quang học có mật độ công suất cao. Hoạt động ở góc tới lướt, thành phần quang học được khắc sâu với một mẫu hai cấp độ của mạng nhiễu xạ cắt và lưới cảm biến mặt sóng Hartmann. Các đặc điểm ngang chặn ánh sáng không mong muốn, cho phép biên độ nhị phân trong phản xạ với độ tương phản mẫu cao. Chúng tôi trình bày đặc điểm bề mặt và phép phản xạ tia X mềm của một mảng mạng nguyên mẫu để chứng minh chức năng trước các ứng dụng đo mặt sóng. Một mô phỏng về hiệu suất thiết bị được hiển thị."} +{"idx": 1870904, "en": "Dynamic self-assembly of micropillars has found wide applications in targeted trapping, micro-crystallization and plasmonic sensing. Yet the efficient fabrication of micropillars array with high flexibility still remains a grand challenge. In this Letter, holographic femtosecond laser multi-foci beams (fs-MFBs) based on a spatial light modulator (SLM) is adopted to efficiently create micropillars array with controllable geometry and spatial distribution by predesigning the computer-generated holograms (CGHs). Based on these micropillars array, diverse hierarchical assemblies are formed under the evaporation-induced capillary force. Moreover, taking advantage of the excellent flexibility and controllability of fs-MFBs, on-demand one-bead-to-one-trap of targeted microspheres at arbitrary position is demonstrated with unprecedentedly high capture efficiency, unfolding their potential applications in the fields of microfluidics and biomedical engineering.", "gemini": "Việc tự lắp ráp động các trụ siêu nhỏ đã tìm thấy ứng dụng rộng rãi trong việc bẫy có mục tiêu, kết tinh siêu nhỏ và cảm biến plasmonic. Tuy nhiên, việc chế tạo hiệu quả mảng trụ siêu nhỏ với độ linh hoạt cao vẫn còn là một thách thức lớn. Trong Thư này, các chùm đa tiêu điểm femtosecond laser (fs-MFB) dựa trên bộ điều biến ánh sáng không gian (SLM) được áp dụng để tạo hiệu quả mảng trụ siêu nhỏ với hình dạng và phân bố không gian có thể kiểm soát được bằng cách thiết kế trước các ảnh ba chiều được tạo bằng máy tính (CGH). Dựa trên mảng trụ siêu nhỏ này, các cụm phân cấp đa dạng được hình thành dưới lực mao dẫn do bay hơi. Hơn nữa, tận dụng tính linh hoạt và khả năng kiểm soát tuyệt vời của fs-MFB, khả năng bẫy một hạt thành một bẫy theo yêu cầu của các vi cầu mục tiêu ở vị trí tùy ý được chứng minh với hiệu suất thu giữ cao chưa từng có, mở ra các ứng dụng tiềm năng của chúng trong các lĩnh vực vi lưu và kỹ thuật y sinh."} +{"idx": 1870905, "en": "The development of integrated vertical III-V nanowire (NW) stimulated emitters in silicon photonics while achieving an efficient light coupling through vertical III-V NW lasers into horizontal optical silicon waveguides is demanding. This is mainly due to the directionality and contradiction of the simultaneously satisfied low threshold stimulated emission conditions of the vertical NWs and efficient light coupling from the NW emitters into the horizontal silicon waveguide. However, we propose a new, to the best of our knowledge, design by taking advantage of resonating features of ring structures and theoretically demonstrate that an interfacial ring resonator between GaAs NW emitters and the silicon waveguide achieves a coupling efficiency up to about 70% at a given wavelength. We also show that the interfacial resonator enables us to adjust the coupling efficiency from about 10% to over 70%. The adjustable coupling efficiency might also be a solution to compromise between the low threshold stimulated emission of NWs and efficient light coupling for realizing efficient silicon couplers based on integrated III-V NW lasers in silicon photonics. Besides the simple fabrication process compared to counterparts, we believe that the novel structure is promising for future optical on-chip data communication in silicon photonics, and the results are expandable to varying wavelengths and materials.", "gemini": "Sự phát triển của bộ phát kích thích dây nano (NW) III-V thẳng đứng tích hợp trong quang tử silicon trong khi đạt được sự ghép nối ánh sáng hiệu quả thông qua laser NW III-V thẳng đứng vào trong các ống dẫn sóng silicon quang học nằm ngang là điều cần thiết. Điều này chủ yếu là do tính định hướng và mâu thuẫn của các điều kiện phát xạ kích thích ngưỡng thấp được thỏa mãn đồng thời của các NW thẳng đứng và sự ghép nối ánh sáng hiệu quả từ các bộ phát NW vào ống dẫn sóng silicon nằm ngang. Tuy nhiên, chúng tôi đề xuất một thiết kế mới, theo hiểu biết tốt nhất của chúng tôi, bằng cách tận dụng các đặc điểm cộng hưởng của các cấu trúc vòng và chứng minh trên lý thuyết rằng một bộ cộng hưởng vòng giao diện giữa các bộ phát NW GaAs và ống dẫn sóng silicon đạt được hiệu suất ghép nối lên tới khoảng 70% ở một bước sóng nhất định. Chúng tôi cũng chỉ ra rằng bộ cộng hưởng giao diện cho phép chúng tôi điều chỉnh hiệu suất ghép nối từ khoảng 10% đến hơn 70%. Hiệu suất ghép nối có thể điều chỉnh cũng có thể là một giải pháp để thỏa hiệp giữa phát xạ kích thích ngưỡng thấp của NW và sự ghép nối ánh sáng hiệu quả để tạo ra các bộ ghép silicon hiệu quả dựa trên laser NW III-V tích hợp trong quang tử silicon. Bên cạnh quy trình chế tạo đơn giản so với các đối tác, chúng tôi tin rằng cấu trúc mới này hứa hẹn cho truyền thông dữ liệu trên chip quang học trong tương lai trong quang tử silicon và kết quả có thể mở rộng đến các bước sóng và vật liệu khác nhau."} +{"idx": 1870906, "en": "Polarization-encoded free-space quantum communication requires a quantum state source featuring fast modulation, long-term stability, and a low intrinsic error rate. Here we present a polarization encoder that, contrary to previous solutions, generates predetermined polarization states with a fixed reference frame in free-space. The proposed device does not require calibration either at the transmitter or at the receiver and achieves long-term stability. A proof-of-concept experiment is also reported, demonstrating a quantum bit error rate lower than 0.2% for several hours without any active recalibration.", "gemini": "Truyền thông lượng tử không gian tự do được mã hóa phân cực đòi hỏi một nguồn trạng thái lượng tử có tính năng điều chế nhanh, độ ổn định dài hạn và tỷ lệ lỗi nội tại thấp. Ở đây chúng tôi trình bày một bộ mã hóa phân cực, trái ngược với các giải pháp trước đây, tạo ra các trạng thái phân cực được xác định trước với một khung tham chiếu cố định trong không gian tự do. Thiết bị được đề xuất không yêu cầu hiệu chuẩn ở máy phát hoặc máy thu và đạt được độ ổn định dài hạn. Một thí nghiệm chứng minh khái niệm cũng được báo cáo, chứng minh tỷ lệ lỗi bit lượng tử thấp hơn 0,2% trong vài giờ mà không cần bất kỳ hiệu chuẩn lại nào."} +{"idx": 1870907, "en": "We address the resonant response and bistability of the exciton-polariton corner states in a higher-order nonlinear topological insulator realized with a kagome arrangement of microcavity pillars. Such states are resonantly excited and exist due to the balance between pump and losses, on one hand, and between nonlinearity and dispersion in inhomogeneous potential landscape, on the other hand, for pump energy around eigen-energies of corresponding linear localized modes. Localization of the nonlinear corner states in a higher-order topological insulator can be efficiently controlled by tuning pump energy. We link the mechanism of corner state formation with symmetry of the truncated kagome array. Corner states coexist with densely packed edge states but are well isolated from them in energy. Nonlinear corner states persist even in the presence of perturbations in a corner microcavity pillar.", "gemini": "Chúng tôi giải quyết phản ứng cộng hưởng và tính lưỡng cực của các trạng thái góc exciton-polariton trong một chất cách điện tôpô phi tuyến bậc cao hơn được thực hiện với sự sắp xếp kagome của các trụ vi khoang. Các trạng thái như vậy được kích thích cộng hưởng và tồn tại do sự cân bằng giữa bơm và tổn thất, một mặt, và giữa phi tuyến tính và sự phân tán trong cảnh quan tiềm năng không đồng nhất, mặt khác, đối với năng lượng bơm xung quanh năng lượng riêng của các chế độ cục bộ tuyến tính tương ứng. Có thể kiểm soát hiệu quả sự cục bộ hóa của các trạng thái góc phi tuyến tính trong chất cách điện tôpô bậc cao hơn bằng cách điều chỉnh năng lượng bơm. Chúng tôi liên kết cơ chế hình thành trạng thái góc với tính đối xứng của mảng kagome bị cắt cụt. Các trạng thái góc cùng tồn tại với các trạng thái cạnh được đóng gói dày đặc nhưng được phân tách tốt khỏi chúng về năng lượng. Các trạng thái góc phi tuyến tính vẫn tồn tại ngay cả khi có nhiễu trong trụ vi khoang góc."} +{"idx": 1870908, "en": "The nonlinear transformation of fluctuations by frequency broadening is found to produce strong anti-correlations in the spectral output. This effect is investigated by dispersive Fourier transform measurements. We exploit the anti-correlations in order to cancel the intensity noise in a subsequent sum-frequency mixing step. This principle allows for the generation of tunable visible pulses by cascaded nonlinear mixing whilst maintaining the same intensity noise performance as the input pulses. In addition, we demonstrate that the power fluctuations occurring in the process of passive stabilization of the carrier-envelope phase locking via difference frequency generation may be cancelled by an analogous strategy.", "gemini": "Phát hiện ra rằng sự biến đổi phi tuyến tính của các biến động bằng cách mở rộng tần số tạo ra các phản tương quan mạnh trong đầu ra phổ. Hiệu ứng này được nghiên cứu bằng các phép đo biến đổi Fourier phân tán. Chúng tôi khai thác các phản tương quan để hủy tiếng ồn cường độ trong bước trộn tần số tổng tiếp theo. Nguyên lý này cho phép tạo ra các xung nhìn thấy có thể điều chỉnh được bằng cách trộn phi tuyến tính theo tầng trong khi vẫn duy trì hiệu suất tiếng ồn cường độ giống như các xung đầu vào. Ngoài ra, chúng tôi chứng minh rằng các biến động công suất xảy ra trong quá trình ổn định thụ động của khóa pha bao thư sóng mang thông qua tạo tần số chênh lệch có thể bị hủy bởi một chiến lược tương tự."} +{"idx": 1870909, "en": "Probabilistic shaping (PS) allows tunable spectral efficiency that is suitable for realizing high throughput intra-data center transceivers. In this Letter, we integrate the cost-minimizing distribution matching (CMDM) in the probability amplitude shaping scheme to generate PS-PAM signals with ultra-short symbol block lengths for reduced serial processing delay. We detail the principle of CMDM and present two different methods of implementation. We demonstrate that CMDM enables the transmission of single wavelength net 200 Gbit/s PS-PAM-8 over 2 km of single-mode fiber (SMF). We show that similar performance is achievable using a constant composition distribution matcher, yet requiring 10 times longer symbol block lengths. We also report, to the best of our knowledge, the first demonstration of net 800 Gbit/s transmission over 2 km of SMF using a packaged 4-λ electro-absorption modulated laser transmitter optical sub-assembly (TOSA).", "gemini": "Việc định hình xác suất (PS) cho phép hiệu suất phổ có thể điều chỉnh phù hợp để hiện thực các bộ thu phát dữ liệu nội bộ trung tâm có thông lượng cao. Trong Thư này, chúng tôi tích hợp việc khớp phân phối giảm thiểu chi phí (CMDM) trong lược đồ định hình biên độ xác suất để tạo ra các tín hiệu PS-PAM với độ dài khối ký hiệu cực ngắn để giảm độ trễ xử lý tuần tự. Chúng tôi trình bày chi tiết nguyên lý của CMDM và trình bày hai phương pháp triển khai khác nhau. Chúng tôi chứng minh rằng CMDM cho phép truyền mạng đơn bước sóng 200 Gbit/s PS-PAM-8 qua 2 km sợi quang đơn mode (SMF). Chúng tôi chỉ ra rằng có thể đạt được hiệu suất tương tự bằng cách sử dụng bộ ghép phân phối thành phần không đổi, nhưng yêu cầu độ dài khối ký hiệu dài hơn 10 lần. Chúng tôi cũng báo cáo, theo hiểu biết tốt nhất của chúng tôi, bản trình diễn đầu tiên về truyền mạng 800 Gbit/s qua 2 km SMF bằng cách sử dụng bộ lắp ráp quang phụ (TOSA) máy phát laser điều chế hấp thụ điện 4-λ đóng gói."} +{"idx": 1870910, "en": "We report on narrowband terahertz (THz) radiation generation via optical rectification from a BaGa4Se7 crystal. The dense phonon mode distribution of the BaGa4Se7 crystal causes narrow transmission bands in the THz frequency range with enhanced nonlinear susceptibility magnitudes, thus permitting strong narrowband THz radiation generation at the frequencies of 1.97 and 2.34 THz. In comparison to THz radiation generated from a ZnTe crystal, the narrowband THz radiation produced by the BaGa4Se7 crystal is 4.5 times higher at 1.97 THz and 63% higher at 2.34 THz, thus making BaGa4Se7 a viable crystal for use in such areas as security and medicine.", "gemini": "Chúng tôi báo cáo về việc tạo ra bức xạ terahertz (THz) hẹp băng thông qua chỉnh lưu quang từ tinh thể BaGa4Se7. Phân bố chế độ phonon dày đặc của tinh thể BaGa4Se7 gây ra các dải truyền hẹp trong dải tần THz với độ lớn độ nhạy phi tuyến được tăng cường, do đó cho phép tạo ra bức xạ THz hẹp băng thông mạnh ở các tần số 1,97 và 2,34 THz. So với bức xạ THz được tạo ra từ tinh thể ZnTe, bức xạ THz hẹp băng thông do tinh thể BaGa4Se7 tạo ra cao hơn 4,5 lần ở tần số 1,97 THz và cao hơn 63% ở tần số 2,34 THz, do đó làm cho BaGa4Se7 trở thành tinh thể khả thi để sử dụng trong các lĩnh vực như an ninh và y học."} +{"idx": 1870911, "en": "For coupled linear cavity-random fiber Raman lasers, for the first time, to the best of our knowledge, we demonstrate a new mechanism of emergence of the random pulses, with the anomalous statistics satisfying optical rogue waves' criteria experimentally. The rogue waves appear as a result of the coupling of two Raman cascades, namely, a linear cavity laser with a wavelength of 1.55 µm and a random laser with a wavelength nearly 1.67 µm, along with coupling of the orthogonal states of polarization (SOPs). The coherent coupling of SOPs causes localization of the trajectories in the vicinity of these states, whereas polarization instability drives escape taking the form of chaotic oscillations. Antiphase dynamics in two cascades result in the suppression of low amplitude chaotic oscillations and enable the anomalous spikes, satisfying rogue waves criteria.", "gemini": "Đối với laser Raman sợi ngẫu nhiên có khoang tuyến tính ghép nối, lần đầu tiên, theo hiểu biết tốt nhất của chúng tôi, chúng tôi chứng minh một cơ chế mới về sự xuất hiện của các xung ngẫu nhiên, với thống kê bất thường đáp ứng các tiêu chí sóng bất thường quang học theo thực nghiệm. Các sóng bất thường xuất hiện do sự ghép nối của hai tầng Raman, cụ thể là laser khoang tuyến tính có bước sóng 1,55 µm và laser ngẫu nhiên có bước sóng gần 1,67 µm, cùng với sự ghép nối các trạng thái phân cực trực giao (SOP). Sự ghép nối mạch lạc của SOP gây ra sự định vị của các quỹ đạo trong vùng lân cận của các trạng thái này, trong khi sự không ổn định phân cực thúc đẩy sự thoát ra dưới dạng dao động hỗn loạn. Động lực ngược pha trong hai tầng dẫn đến sự ức chế các dao động hỗn loạn biên độ thấp và cho phép các đột biến bất thường, đáp ứng các tiêu chí sóng bất thường."} +{"idx": 1870912, "en": "The wave nature and diffraction of light pose a significant bottleneck to the continued performance and efficiency scaling of a wide variety of integrated photonic devices, often necessitating solutions based on resonance, slow-light, or plasmonics to derive enhanced light-matter interaction. Here, we introduce all-dielectric waveguides that exploit the vectorial nature of light to achieve strong subdiffraction confinement in high index dielectrics, enabling characteristic mode dimensions below λ02/1000 without metals or plasmonics. We further show how these ultra-small mode areas may coincide or diverge from the nonlinear effective mode area. The work opens the door to new types of waveguide-based devices featuring strong near-field confinement, Purcell factors, and nonlinear effects, with broad applications spanning classical and quantum optics.", "gemini": "Bản chất sóng và sự nhiễu xạ của ánh sáng gây ra nút thắt cổ chai đáng kể đối với hiệu suất liên tục và hiệu quả mở rộng quy mô của nhiều loại thiết bị quang học tích hợp, thường cần các giải pháp dựa trên cộng hưởng, ánh sáng chậm hoặc plasmonics để có được sự tương tác ánh sáng-vật chất được cải thiện. Tại đây, chúng tôi giới thiệu các ống dẫn sóng toàn điện môi khai thác bản chất vectơ của ánh sáng để đạt được sự hạn chế dưới nhiễu xạ mạnh trong điện môi chỉ số cao, cho phép kích thước chế độ đặc trưng dưới λ02/1000 mà không cần kim loại hoặc plasmonics. Hơn nữa, chúng tôi chỉ ra cách các vùng chế độ cực nhỏ này có thể trùng khớp hoặc khác biệt với vùng chế độ hiệu dụng phi tuyến tính. Công trình này mở ra cánh cửa cho các loại thiết bị dựa trên ống dẫn sóng mới có tính năng hạn chế trường gần mạnh, các yếu tố Purcell và các hiệu ứng phi tuyến tính, với các ứng dụng rộng rãi trải dài trên quang học cổ điển và lượng tử."} +{"idx": 1870913, "en": "Real-time 3-D tracking of a fast-moving object has found important applications in industry, traffic control, sports, biomedicine, defense, etc. However, it is difficult to adopt typical image-based object tracking systems in a fast-moving object tracking in real time and for a long duration, because reliable and robust image processing and analysis algorithms are often computationally exhausted, and limited storage and bandwidth can hardly fulfill the great demand of high-speed photography. Here we report an image-free 3-D tracking approach. The approach uses only two single-pixel detectors and a high-speed spatial light modulator for data acquisition. By illuminating the target moving object with six single-period Fourier basis patterns, the approach is able to analytically calculate the position of the object with the corresponding single-pixel measurements. The approach is low-cost, and data- and computation-efficient. We experimentally demonstrate that the proposed approach can detect and track a fast-moving object at a frame rate of 1666 frames per second by using a 10,000 Hz digital micromirror device. Benefiting from the wide working spectrum of single-pixel detectors, the reported approach might be applicable for hidden fast-moving object tracking.", "gemini": "Việc theo dõi 3D thời gian thực đối với một vật thể chuyển động nhanh đã tìm thấy các ứng dụng quan trọng trong công nghiệp, kiểm soát giao thông, thể thao, y sinh, quốc phòng, v.v. Tuy nhiên, rất khó để áp dụng các hệ thống theo dõi đối tượng dựa trên hình ảnh thông thường trong việc theo dõi đối tượng chuyển động nhanh theo thời gian thực và trong thời gian dài, bởi vì các thuật toán xử lý và phân tích hình ảnh đáng tin cậy và mạnh mẽ thường bị cạn kiệt về mặt tính toán và lưu trữ hạn chế và băng thông khó có thể đáp ứng được nhu cầu lớn về nhiếp ảnh tốc độ cao. Tại đây, chúng tôi báo cáo một phương pháp theo dõi 3D không cần hình ảnh. Phương pháp này chỉ sử dụng hai máy dò pixel đơn và một bộ điều biến ánh sáng không gian tốc độ cao để thu thập dữ liệu. Bằng cách chiếu sáng vật thể chuyển động mục tiêu bằng sáu mẫu cơ sở Fourier chu kỳ đơn, phương pháp này có khả năng tính toán phân tích vị trí của vật thể với các phép đo pixel đơn tương ứng. Phương pháp này có chi phí thấp, hiệu quả về dữ liệu và tính toán. Chúng tôi chứng minh bằng thực nghiệm rằng phương pháp được đề xuất có thể phát hiện và theo dõi một vật thể chuyển động nhanh với tốc độ khung hình là 1666 khung hình mỗi giây bằng cách sử dụng thiết bị micromirror kỹ thuật số 10.000 Hz. Được hưởng lợi từ phổ làm việc rộng của các máy dò pixel đơn, phương pháp được báo cáo có thể áp dụng để theo dõi đối tượng chuyển động nhanh ẩn."} +{"idx": 1870914, "en": "The full width at half maximum (FWHM) of lossy mode resonances (LMRs) in the optical spectrum depends on the homogeneity of the thin film deposited. In this Letter, a method for improving the FWHM is applied for an LMR generated by a D-shaped optical fiber in reflection configuration. For this purpose, three samples with different attenuation were deposited with DC sputtering thin films of SnO2-, and a further controlled immersion of the samples in water was performed. A laser-cleaner method was used to improve the FWHM characteristics of one of the samples from 106 to 53 nm. This improvement can be applied to thin-film-based sensors where there is a problem with the inhomogeneity of the coating thickness. Moreover, with this technique, it was proved that a coated length of just 3-4 mm permits the generation of an LMR, with implications for the miniaturization of the final device.", "gemini": "Chiều rộng toàn bộ ở nửa cực đại (FWHM) của cộng hưởng chế độ mất mát (LMR) trong quang phổ quang học phụ thuộc vào tính đồng nhất của màng mỏng được lắng đọng. Trong Thư này, một phương pháp cải thiện FWHM được áp dụng cho LMR được tạo bởi sợi quang hình chữ D trong cấu hình phản xạ. Với mục đích này, ba mẫu có độ suy giảm khác nhau đã được lắng đọng bằng màng mỏng SnO2- phun DC và tiếp tục ngâm các mẫu trong nước một cách có kiểm soát. Một phương pháp làm sạch bằng laser đã được sử dụng để cải thiện các đặc tính FWHM của một trong các mẫu từ 106 xuống 53 nm. Sự cải tiến này có thể được áp dụng cho các cảm biến dựa trên màng mỏng khi có vấn đề về tính không đồng nhất của độ dày lớp phủ. Hơn nữa, với kỹ thuật này, đã chứng minh rằng chiều dài lớp phủ chỉ 3-4 mm cho phép tạo ra LMR, với ý nghĩa đối với việc thu nhỏ thiết bị cuối cùng."} +{"idx": 1870915, "en": "A general eigen equation has been deduced that can handle ideal and non-ideal quarter-wavelength cases of Bragg-reflection waveguide structure for nonlinear interaction with matching layers on each side of the active region. In particular, this equation allows for solving the cases when the Bragg reflectors are non-ideal quarter-wavelength thick. With this equation, we propose a Bragg-reflection waveguide structure based on the AlxGa1-As/GaAs material system with high figure-of-merit |ξd| total nonlinearity and checked the influence if the thickness of the quarter-wavelength Bragg reflectors is off by 10%.", "gemini": "Một phương trình riêng tổng quát đã được suy ra, có thể xử lý các trường hợp bước sóng một phần tư lý tưởng và không lý tưởng của cấu trúc hướng dẫn sóng phản xạ Bragg để tương tác phi tuyến tính với các lớp khớp ở mỗi bên của vùng hoạt động. Cụ thể, phương trình này cho phép giải các trường hợp khi các phản xạ Bragg dày hơn bước sóng một phần tư không lý tưởng. Với phương trình này, chúng tôi đề xuất một cấu trúc hướng dẫn sóng phản xạ Bragg dựa trên hệ vật liệu AlxGa1-As/GaAs với hệ số công trạng cao |ξd| phi tuyến tính tổng và kiểm tra ảnh hưởng nếu độ dày của các phản xạ Bragg bước sóng một phần tư chênh lệch 10%."} +{"idx": 1870916, "en": "A highly sensitive surface plasmon resonance fiber sensor for a vector magnetic field is proposed. The sensor is composed of a half-side gold-coated multimode-single-mode-multimode hetero-core fiber structure encapsulated with ferrofluids. The half-side gold film on the fiber not only produces the surface plasmon resonance, but also breaks the centrosymmetry of the light field in the fiber. Moreover, the magnetic-field-dependent anisotropy of the surrounding ferrofluids makes the sensor sensitive to both the intensity and direction of the magnetic field. Owing to the unique half-side coating configuration and the resulting enhancement of the evanescent field, the sensor can achieve a sensitivity as high as 1008 pm/Oe to the magnetic field intensity. The proposed sensor, possessing advantages such as high sensitivity, ease of fabrication, and low cost, has potential in the detection of a weak vector magnetic field.", "gemini": "Một cảm biến sợi quang cộng hưởng plasmon bề mặt có độ nhạy cao cho trường từ vectơ được đề xuất. Cảm biến bao gồm một cấu trúc sợi đa chế độ-đơn chế độ-đa chế độ lõi dị được phủ một nửa bằng vàng được bọc bằng chất lỏng sắt từ. Lớp vàng một nửa trên sợi không chỉ tạo ra cộng hưởng plasmon bề mặt mà còn phá vỡ tính đối xứng tâm của trường ánh sáng trong sợi. Hơn nữa, tính dị hướng phụ thuộc vào từ trường của chất lỏng sắt từ xung quanh làm cho cảm biến nhạy với cả cường độ và hướng của từ trường. Nhờ cấu hình lớp phủ một nửa độc đáo và sự tăng cường trường lan truyền do đó, cảm biến có thể đạt được độ nhạy cao tới 1008 pm/Oe đối với cường độ từ trường. Cảm biến được đề xuất, sở hữu những ưu điểm như độ nhạy cao, dễ chế tạo và chi phí thấp, có tiềm năng trong việc phát hiện từ trường vectơ yếu."} +{"idx": 1870917, "en": "We provide a novel approach for estimating the modal and chromatic dispersions of the LP01 and LP11 modes traversing a two-mode fiber (TMF). A modal interferometer is used to measure the differential group delay (DGD) and chromatic dispersion for the two modes in the 1260-1360 nm telecommunication band. The measurement principle is based on an investigation of a transmitted spectrum through temporal decomposition by means of a Fourier transform. The diagnosis of the DGD and chromatic dispersion for the two modes is discussed theoretically and experimentally. The experimental results obtained here exactly match those obtained previously. The salient feature of the present method is that the modal interferometer configuration makes it possible to measure both the DGD and chromatic dispersion and also estimate the shape of optical pulses traversing a TMF.", "gemini": "Chúng tôi cung cấp một phương pháp tiếp cận mới để ước tính độ phân tán mô và độ phân tán màu của các chế độ LP01 và LP11 đi qua một sợi hai chế độ (TMF). Một giao thoa kế mô được sử dụng để đo độ trễ nhóm vi sai (DGD) và độ phân tán màu cho hai chế độ trong băng viễn thông 1260-1360 nm. Nguyên lý đo dựa trên việc nghiên cứu phổ truyền qua thông qua phân rã thời gian bằng biến đổi Fourier. Việc chẩn đoán DGD và độ phân tán màu cho hai chế độ được thảo luận về mặt lý thuyết và thực nghiệm. Các kết quả thực nghiệm thu được ở đây hoàn toàn trùng khớp với những kết quả thu được trước đây. Đặc điểm nổi bật của phương pháp hiện nay là cấu hình giao thoa kế mô giúp có thể đo được cả DGD và độ phân tán màu cũng như ước tính hình dạng của các xung quang đi qua TMF."} +{"idx": 1870918, "en": "A broadband and low-dispersion high refractive index (HRI) metamaterial formed by symmetrically etching two identical metasurfaces on both sides of a dielectric slab has been numerically and experimentally demonstrated in the terahertz region. The unit cell of the metasurface is a Jerusalem cross surrounded by a square metal ring, in which there are two magnetic resonances and one electric resonance. The proposed metamaterial simultaneously possesses high effective permittivity and permeability in broadband frequencies, since the multiple resonances result in a significant bandwidth expansion of a HRI. The simulation results reveal that the refractive index of the proposed metamaterial reaches up to 27 in the frequency range of 0.39-0.65 THz, and the relative bandwidth is about 44%. Furthermore, the fluctuation of the refractive index in this frequency band is less than 6%, showing a good low-dispersion characteristic. We also fabricated a sample to verify this HRI property. Experimental results are in good agreement with numerical simulations. This broadband HRI metamaterial is desirable in many fields, such as in high-resolution imaging and optical communications.", "gemini": "Một siêu vật liệu có chiết suất cao (HRI) có độ phân tán thấp và băng thông rộng được hình thành bằng cách khắc đối xứng hai siêu bề mặt giống hệt nhau ở cả hai mặt của một tấm điện môi đã được chứng minh bằng số và thực nghiệm trong vùng terahertz. Ô đơn vị của siêu bề mặt là một chữ thập Jerusalem được bao quanh bởi một vòng kim loại hình vuông, trong đó có hai cộng hưởng từ và một cộng hưởng điện. Siêu vật liệu được đề xuất đồng thời sở hữu độ thấm và độ cho phép hiệu dụng cao trong tần số băng thông rộng, vì nhiều cộng hưởng dẫn đến mở rộng đáng kể băng thông của HRI. Kết quả mô phỏng cho thấy chiết suất của siêu vật liệu được đề xuất đạt tới 27 trong dải tần 0,39-0,65 THz và băng thông tương đối là khoảng 44%. Hơn nữa, sự biến động của chiết suất trong dải tần này nhỏ hơn 6%, cho thấy đặc tính phân tán thấp tốt. Chúng tôi cũng chế tạo một mẫu để xác minh đặc tính HRI này. Kết quả thực nghiệm phù hợp tốt với mô phỏng số. Siêu vật liệu HRI băng thông rộng này được mong muốn trong nhiều lĩnh vực, chẳng hạn như trong hình ảnh có độ phân giải cao và truyền thông quang."} +{"idx": 1870919, "en": "We employ dual-probe one-dimensional (1D) femtosecond (fs)/picosecond (ps) hybrid rotational coherent anti-Stokes Raman spectroscopy (HRCARS) to investigate simultaneous temperature, pressure, and O2/N2 measurements for gas-phase diagnostics. The dual-probe HRCARS technique allows for simultaneous measurements from the time and frequency-domain. A novel approach for measuring pressure, which offers high accuracy (<1%) and precision (0.42%), is presented. The technique is first demonstrated in a chamber for a range of pressures (1-1.5 bar). This technique shows an impressive capability of resolving 1D pressure gradients arising from a N2 jet impinging on a surface, both in laminar and turbulent conditions. The technique is shown to be capable of resolving single-shot pressure gradients (0.04 bar/mm) originating from kinetic energy conversion to pressure and resolves characteristic O2/N2 structures from laminar and turbulent mixing.", "gemini": "Chúng tôi sử dụng quang phổ Raman phản Stokes ăn nhập (HRCARS) quay một chiều (1D) femtosani (fs)/picosani (ps) một đầu dò kép để nghiên cứu đồng thời nhiệt độ, áp suất và phép đo O2/N2 để chẩn đoán pha khí. Kỹ thuật HRCARS hai đầu dò cho phép đo đồng thời từ thời gian và miền tần số. Một phương pháp mới để đo áp suất, mang lại độ chính xác cao (<1%) và độ chính xác (0,42%), được trình bày. Kỹ thuật này lần đầu tiên được trình diễn trong một buồng cho một phạm vi áp suất (1-1,5 bar). Kỹ thuật này cho thấy khả năng ấn tượng trong việc giải quyết các gradien áp suất 1D phát sinh từ tia N2 đập vào bề mặt, cả trong điều kiện dòng chảy tầng và nhiễu động. Kỹ thuật này được chứng minh là có khả năng giải quyết các gradien áp suất một lần (0,04 bar/mm) có nguồn gốc từ chuyển đổi năng lượng động học thành áp suất và giải quyết các cấu trúc O2/N2 đặc trưng từ sự trộn lẫn dòng chảy tầng và nhiễu động."} +{"idx": 1870920, "en": "We propose and numerically demonstrate a scheme of coherent optical chaos communication using semiconductor lasers for secure transmission of optical quadrature amplitude modulation (QAM) signals. In this scheme, a laser intensity chaos and its delayed duplicate are used to amplitude-quadrature modulate a continuous-wave light to generate a chaotic carrier. High-quality chaotic carrier synchronization between the transmitter and receiver is guaranteed by laser intensity chaos synchronization, avoiding laser phase fluctuation. Decryption is implemented by a 90 deg optical hybrid using the synchronous chaotic carrier as local light. Secure transmission of an optical 40 Gb/s 16QAM signal is demonstrated by using a laser intensity chaos with a bandwidth of 11.7 GHz. The system performances are evaluated by analyzing a bit error ratio with different masking coefficients, signal rates, synchronization coefficients, parameter mismatches, and dispersion compensation. It is believed that this scheme can pave a way for high-speed optical chaos communication.", "gemini": "Chúng tôi đề xuất và chứng minh bằng số một lược đồ truyền thông hỗn loạn quang học mạch lạc sử dụng laser bán dẫn để truyền tín hiệu điều chế biên độ pha phương sai quang (QAM) một cách an toàn. Trong lược đồ này, hỗn loạn cường độ laser và bản sao trễ của nó được sử dụng để điều chế biên độ-pha phương sai một ánh sáng sóng liên tục để tạo ra sóng mang hỗn loạn. Đồng bộ sóng mang hỗn loạn chất lượng cao giữa máy phát và máy thu được đảm bảo bằng đồng bộ hỗn loạn cường độ laser, tránh biến động pha laser. Giải mã được thực hiện bằng một lai quang 90 độ sử dụng sóng mang hỗn loạn đồng bộ làm ánh sáng cục bộ. Truyền tín hiệu an toàn của tín hiệu quang 40 Gb/giây 16QAM được chứng minh bằng cách sử dụng hỗn loạn cường độ laser có băng thông 11,7 GHz. Hiệu suất của hệ thống được đánh giá bằng cách phân tích tỷ lệ lỗi bit với các hệ số che chắn, tốc độ tín hiệu, hệ số đồng bộ, sai số tham số và bù độ phân tán khác nhau. Người ta tin rằng lược đồ này có thể mở đường cho truyền thông hỗn loạn quang tốc độ cao."} +{"idx": 1870921, "en": "While optical coherence tomography (OCT) provides a resolution down to 1 µm, it has difficulties in visualizing cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is the site-dependent active motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT), which we believe has crucial advantages. Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology, much faster and more efficiently than FF-OCT. Both shorter acquisition times and tomographic depth-sectioning reduce the sensitivity of dynamic contrast for bulk tissue motion artifacts and simplify their correction in post-processing. Dynamic contrast makes microscopic FD-OCT a promising tool for the histological analysis of unstained tissues.", "gemini": "Mặc dù chụp cắt lớp quang học (OCT) cung cấp độ phân giải xuống tới 1 µm, nhưng nó gặp khó khăn trong việc hình dung các cấu trúc tế bào do thiếu độ tương phản tán xạ. Bằng cách đánh giá các biến động tín hiệu, độ tương phản được cải thiện đáng kể đã được chứng minh bằng cách sử dụng OCT trường đầy đủ miền thời gian (FF-OCT), giúp các cấu trúc tế bào và dưới tế bào có thể nhìn thấy được. Nguyên nhân giả định của tín hiệu OCT động là chuyển động chủ động theo vị trí của các cấu trúc tế bào trong phạm vi dưới micrômet, cung cấp độ tương phản giống như mô học. Ở đây chúng tôi trình bày độ tương phản động với OCT miền tần số quét (FD-OCT), mà chúng tôi tin rằng có những ưu điểm quan trọng. Với hình dạng hình ảnh cắt ngang vốn có, FD-OCT quét cung cấp hình ảnh có độ phân giải theo chiều sâu trên các lớp mô, một góc nhìn được biết đến từ mô bệnh học, nhanh hơn và hiệu quả hơn nhiều so với FF-OCT. Cả thời gian thu thập ngắn hơn và cắt lớp theo chiều sâu đều làm giảm độ nhạy của độ tương phản động đối với các hiện vật chuyển động của mô khối và đơn giản hóa quá trình hiệu chỉnh của chúng trong xử lý hậu kỳ. Độ tương phản động làm cho FD-OCT dưới kính hiển vi trở thành một công cụ đầy hứa hẹn để phân tích mô học các mô chưa nhuộm."} +{"idx": 1870922, "en": "The direct imaging of tissue preserved in formalin-fixed paraffin-embedded (FFPE) blocks remains a challenge. There are presently millions of tissues preserved as FFPE blocks whose assessment via bright-field microscopes requires them to be sectioned and subsequently stained. These processes are laborious, resource-intensive, and time consuming. In this Letter, we utilize an ultraviolet laser with photoacoustic remote sensing to provide a novel method that enables direct label-free pathological assessment of FFPE blocks. We demonstrate the efficacy of this technique by imaging human breast tissue, highlighting salient features such as ducts, adipocytes, and ductal hyperplasia. This direct imaging of FFPE blocks facilitates pathological assessment much earlier in the histopathological workflow, saving valuable time in clinical and research settings. The presented non-contact label-free reflection-mode device enables augmentation of existing histopathological workflows and aims to expand the arsenal of imaging technologies available to clinicians.", "gemini": "Việc chụp ảnh trực tiếp các mô được bảo quản trong các khối nhuộm parafin cố định bằng formalin (FFPE) vẫn là một thách thức. Hiện tại có hàng triệu mô được bảo quản dưới dạng các khối FFPE mà việc đánh giá chúng thông qua kính hiển vi trường sáng đòi hỏi phải cắt lát và sau đó nhuộm. Các quy trình này rất tốn công, tốn nhiều tài nguyên và thời gian. Trong Thư này, chúng tôi sử dụng tia laser cực tím với cảm biến từ xa quang âm để cung cấp một phương pháp mới cho phép đánh giá bệnh lý trực tiếp không cần nhãn của các khối FFPE. Chúng tôi chứng minh hiệu quả của kỹ thuật này bằng cách chụp ảnh mô vú của người, làm nổi bật các đặc điểm nổi bật như ống dẫn, tế bào mỡ và tăng sản ống dẫn. Việc chụp ảnh trực tiếp các khối FFPE này giúp việc đánh giá bệnh lý dễ dàng hơn nhiều trong quy trình làm việc mô bệnh học, tiết kiệm thời gian quý báu trong các công tác lâm sàng và nghiên cứu. Thiết bị chế độ phản xạ không tiếp xúc không nhãn được trình bày cho phép tăng cường quy trình làm việc mô bệnh học hiện có và nhằm mở rộng kho vũ khí các công nghệ hình ảnh có sẵn cho các bác sĩ lâm sàng."} +{"idx": 1870923, "en": "We propose a simple, highly scalable, and very efficient scheme for coherent combining of tiled aperture arrays. The scheme relies on changing the beam phasing paradigm from the commonly used in-phase pattern to the out-of-phase pattern (interleaved 0/π phases in the neighboring channels) and using an additional simple combining stage (a beamsplitter). In a proof-of-concept experiment with a one-dimensional fiber array, we achieved 89% of the power in the main combined beam. In numerical modeling, we found optimal conditions leading to 98% efficiency for an unlimited number of channels and arbitrary small initial aperture fill factors. The scheme is highly resistant to the effect of sub-aperture clipping and suitable for combining ultrashort pulses.", "gemini": "Chúng tôi đề xuất một lược đồ đơn giản, có khả năng mở rộng cao và rất hiệu quả để kết hợp mạch lạc các mảng khẩu độ xếp. Lược đồ dựa trên việc thay đổi mô hình pha chùm từ mô hình đồng pha thường được sử dụng sang mô hình lệch pha (các pha 0/π xen kẽ trong các kênh lân cận) và sử dụng một giai đoạn kết hợp đơn giản bổ sung (bộ chia chùm). Trong một thí nghiệm chứng minh khái niệm với một mảng sợi một chiều, chúng tôi đạt được 89% công suất trong chùm kết hợp chính. Trong mô hình số, chúng tôi tìm thấy các điều kiện tối ưu dẫn đến hiệu suất 98% cho số lượng kênh không giới hạn và các yếu tố lấp đầy khẩu độ ban đầu nhỏ tùy ý. Lược đồ có khả năng chống chịu cao với hiệu ứng cắt khẩu độ phụ và phù hợp để kết hợp các xung cực ngắn."} +{"idx": 1870924, "en": "Real-time spectrum sensing is crucial to enable dynamic spectrum sharing for ubiquitous wireless communication with seamless high-quality wireless services. In this contribution, we demonstrate a reconfigurable real-time spectrum sensing photonic integrated chip (RTSS-PIC) for millimeter-wave (mm-wave) signals, based on the temporal discrimination of multi-channel input frequencies by the peak group delays of cascaded ring resonators. Large group delays and wide bandwidths are realized by the strategic tuning of thermal phase shifters. To make the spectrum sensing fully reconfigurable, positive and negative group delay profiles are utilized. Furthermore, a unique frequency-to-time mapping technique is introduced to analyze all input channels in real time. To illustrate the method, real-time spectrum sensing for two different mm-wave signals are finally tested by full-wave simulations of the proposed RTSS-PIC.", "gemini": "Cảm biến phổ thời gian thực đóng vai trò quan trọng để cho phép chia sẻ phổ động đối với truyền thông không dây phổ biến với các dịch vụ không dây chất lượng cao liền mạch. Trong đóng góp này, chúng tôi trình bày một chip tích hợp quang tử cảm biến phổ thời gian thực có thể định hình lại (RTSS-PIC) cho các tín hiệu sóng milimet (mm-wave), dựa trên sự phân biệt thời gian của các tần số đầu vào đa kênh theo độ trễ nhóm đỉnh của các cộng hưởng vòng nối tiếp. Độ trễ nhóm lớn và băng thông rộng được thực hiện bằng cách điều chỉnh chiến lược các bộ dịch pha nhiệt. Để làm cho cảm biến phổ có thể định hình lại hoàn toàn, các cấu hình độ trễ nhóm dương và âm được sử dụng. Hơn nữa, một kỹ thuật ánh xạ tần số sang thời gian độc đáo được giới thiệu để phân tích tất cả các kênh đầu vào theo thời gian thực. Để minh họa phương pháp, cảm biến phổ thời gian thực cho hai tín hiệu mm-wave khác nhau cuối cùng được thử nghiệm bằng các mô phỏng sóng đầy đủ của RTSS-PIC được đề xuất."} +{"idx": 1870925, "en": "Based on the experimental platform of an L-band normal-dispersion mode-locked fiber laser, we report the first observation, to our knowledge, of three novel types of multi-soliton asynchronous pulsation phenomena by virtue of the dispersive Fourier transform technique. The experimental results provide new insights into the complex multi-soliton dynamics under unstable mode-locking conditions. It is confirmed that more than one pulsating solution can coexist in a multi-pulse situation and that each soliton may evolve periodically in different ways. This implies that subsequent experimental and theoretical studies on multi-soliton need to take the differences among pulses into account and retrieve more degrees of freedom.", "gemini": "Dựa trên nền tảng thử nghiệm của laser sợi khóa chế độ phân tán bình thường băng tần L, chúng tôi báo cáo quan sát đầu tiên, theo hiểu biết của chúng tôi, về ba loại mới của hiện tượng xung đa soliton không đồng bộ nhờ kỹ thuật biến đổi Fourier phân tán. Các kết quả thực nghiệm cung cấp những hiểu biết mới về động lực học đa soliton phức tạp trong các điều kiện khóa chế độ không ổn định. Được xác nhận rằng nhiều hơn một giải pháp xung có thể cùng tồn tại trong tình huống nhiều xung và mỗi soliton có thể tiến hóa theo chu kỳ theo những cách khác nhau. Điều này ngụ ý rằng các nghiên cứu thực nghiệm và lý thuyết tiếp theo về đa soliton cần tính đến sự khác biệt giữa các xung và khôi phục nhiều bậc tự do hơn."} +{"idx": 1870926, "en": "Metal halide perovskite light-emitting diodes (PeLEDs) have experienced a rapid advancement in the last several years with the external quantum efficiencies (EQEs) reaching over 20%, comparable to the state-of-the-art organic LEDs and quantum dot LEDs. The photoluminescence quantum yields of perovskite films have also been approaching 100%. Therefore, the next step to improving the EQE of PeLEDs should be focused on boosting light extraction. In this Letter, we demonstrate the emitter dipole orientation as a key parameter in determining the outcoupling efficiency of PeLEDs. We find that the CsPbBr3 emitter has a slightly preferred orientation with the horizontal-to-vertical dipole ratio of 0.41:0.59, as compared to 0.33:0.67 in the isotropic case. A theoretical analysis predicts that a purely anisotropic perovskite emitter may result in a maximum EQE of 36%.", "gemini": "Điốt phát sáng perovskite halide kim loại (PeLED) đã trải qua sự tiến bộ nhanh chóng trong vài năm trở lại đây với hiệu suất lượng tử ngoài (EQE) đạt trên 20%, tương đương với đèn LED hữu cơ và đèn LED chấm lượng tử hiện đại. Hiệu suất lượng tử phát quang của màng perovskite cũng đã đạt gần 100%. Do đó, bước tiếp theo để cải thiện EQE của PeLED nên tập trung vào việc tăng cường trích xuất ánh sáng. Trong Thư này, chúng tôi trình bày hướng của lưỡng cực phát ra như một thông số chính để xác định hiệu suất kết nối ngoài của PeLED. Chúng tôi thấy rằng bộ phát CsPbBr3 có hướng ưu tiên hơi nghiêng với tỷ lệ lưỡng cực ngang-dọc là 0,41:0,59, so với 0,33:0,67 trong trường hợp đẳng hướng. Một phân tích lý thuyết dự đoán rằng một bộ phát perovskite hoàn toàn dị hướng có thể dẫn đến EQE tối đa là 36%."} +{"idx": 1870927, "en": "Optical beams carrying orbital angular momentum are a very active field of research for their prospective applications, especially at short wavelengths. We consider here such beams produced through high-harmonic generation (HHG) in a rare gas and analyze the characterization of their high-charge vortex structure by an extreme ultraviolet Hartmann wavefront sensor. We show that such HHG beams are generally composed of a set of numerous vortex modes. The sensitivity of the intensity and phase of the HHG beam to the infrared laser aberrations is investigated using a deformable mirror.", "gemini": "Các chùm tia quang mang động lượng góc quỹ đạo là một lĩnh vực nghiên cứu rất sôi động vì các ứng dụng tiềm năng của chúng, đặc biệt là ở các bước sóng ngắn. Ở đây, chúng tôi xem xét các chùm tia như vậy được tạo ra thông qua quá trình tạo sóng hài bậc cao (HHG) trong một loại khí hiếm và phân tích đặc điểm của cấu trúc vòng xoáy có điện tích cao của chúng bằng cảm biến mặt sóng Hartmann cực tím. Chúng tôi chỉ ra rằng các chùm HHG như vậy thường bao gồm một tập hợp nhiều chế độ xoáy. Độ nhạy của cường độ và pha của chùm HHG đối với quang sai laser hồng ngoại được nghiên cứu bằng cách sử dụng một gương biến dạng."} +{"idx": 1870928, "en": "Optical cavities with high figure of merit Q/V is essential to enhance the interaction of light and matter. Here, a hybrid photonic-plasmonic nano-cavity, consisting of an L3 photonic crystal nano-cavity and plasmonic bowtie nano-antennas, is proposed to have an ultrahigh figure of merit Q/V of 8.4×106(λ/n)-3, which is the highest value ever demonstrated for all previous works about L3-type photonic crystal nano-cavities. The value of Q/V is enhanced by more than 25 times compared to that in a bare L3 photonic crystal nano-cavity and is 60 times greater than plasmonic bowtie nano-antennas. As a result, the single-atom cooperativity parameter is improved by 26 times with respect to a bare L3 photonic crystal nano-cavity, and strong coupling between light and a single emitter is achieved. The proposed structure provides a new platform to achieve strong coupling between light and a single emitter, which holds great potential for applications in quantum optics, quantum information, and nonlinear optics.", "gemini": "Các khoang quang có hệ số phẩm chất Q/V cao rất cần thiết để tăng cường sự tương tác của ánh sáng và vật chất. Ở đây, một nano-khoang photon-plasmon lai, bao gồm một nano-khoang photon tinh thể L3 và nano-ăng ten hình nơ plasmon, được đề xuất có hệ số phẩm chất cực cao Q/V là 8,4×106(λ/n)-3, đây là giá trị cao nhất từng được chứng minh cho tất cả các công trình trước đây về nano-khoang tinh thể photon loại L3. Giá trị Q/V được tăng cường hơn 25 lần so với nano-khoang tinh thể photon L3 trần và lớn hơn 60 lần so với nano-ăng ten hình nơ plasmon. Kết quả là, thông số hợp tác đơn nguyên tử được cải thiện 26 lần so với nano-khoang tinh thể photon L3 trần và đạt được sự ghép nối mạnh giữa ánh sáng và một bộ phát đơn. Cấu trúc được đề xuất cung cấp một nền tảng mới để đạt được sự ghép nối mạnh giữa ánh sáng và một bộ phát đơn, có tiềm năng lớn cho các ứng dụng trong quang học lượng tử, thông tin lượng tử và quang học phi tuyến."} +{"idx": 1870929, "en": "In this Letter, we present a novel, to the best of our knowledge, single-shot method for characterizing focused coherent beams. We utilize a dedicated amplitude-only mask, in combination with an iterative phase retrieval algorithm, to reconstruct the amplitude and phase of a focused beam from a single measured far-field diffraction pattern alone. In a proof-of-principle experiment at a wavelength of 13.5 nm, we demonstrate our new method and obtain an RMS phase error of better than λ/70. This method will find applications in the alignment of complex optical systems, real-time feedback to adaptive optics, and single-shot beam characterization, e.g., at free-electron lasers or high-order harmonic beamlines.", "gemini": "Trong Thư này, chúng tôi trình bày một phương pháp mới, theo hiểu biết tốt nhất của chúng tôi, phương pháp chụp đơn để đặc trưng các chùm tia hội tụ liên kết. Chúng tôi sử dụng mặt nạ biên độ chuyên dụng, kết hợp với thuật toán truy hồi pha lặp, để tái tạo biên độ và pha của chùm tia hội tụ chỉ từ một mẫu nhiễu xạ trường xa được đo duy nhất. Trong một thí nghiệm chứng minh nguyên lý ở bước sóng 13,5 nm, chúng tôi trình bày phương pháp mới của mình và thu được sai số pha RMS tốt hơn λ/70. Phương pháp này sẽ tìm thấy các ứng dụng trong việc căn chỉnh các hệ thống quang phức tạp, phản hồi thời gian thực cho quang học thích ứng và đặc tính chùm tia chụp đơn, ví dụ như ở laser electron tự do hoặc các đường chùm sóng hài bậc cao."} +{"idx": 1870930, "en": "Stimulated Brillouin scattering (SBS) has significant influence on optical fiber communication (OFC), optical fiber sensing (OFS), and narrow-linewidth fiber laser (NLFL) systems. How to effectively suppress it has always been a challenge. In this Letter, we propose and demonstrate a versatile solution, for the first time, to the best of our knowledge, by using tilted fiber Bragg gratings (TFBGs). A specially designed and fabricated TFBG can be used as an ultra-narrow spectral filter, precisely matching with the operation laser wavelength and the tiny frequency shift due to SBS. Experimental results show that the backward Stokes can be strongly rejected with a filtering ratio of >10dB; meanwhile, an obvious increasing of SBS threshold is observed with a maximum value of 1.7 times that without the TFBG, which enhances the effective transmission power by 33%. The operation stability of this method also is validated. This work opens new opportunities for SBS suppression in OFC, OFS, and high-power NLFL systems.", "gemini": "Tán xạ Brillouin kích thích (SBS) có ảnh hưởng đáng kể đến hệ thống truyền thông sợi quang (OFC), cảm biến sợi quang (OFS) và laser sợi hẹp (NLFL). Làm thế nào để ức chế hiệu quả nó luôn là một thách thức. Trong Thư này, chúng tôi đề xuất và trình bày một giải pháp linh hoạt, lần đầu tiên, theo hiểu biết của chúng tôi, bằng cách sử dụng các mạng Bragg sợi nghiêng (TFBG). Một TFBG được thiết kế và chế tạo đặc biệt có thể được sử dụng như một bộ lọc quang phổ cực hẹp, khớp chính xác với bước sóng laser hoạt động và độ dịch tần nhỏ do SBS. Kết quả thực nghiệm cho thấy Stokes ngược có thể bị loại bỏ mạnh mẽ với tỷ lệ lọc> 10dB; trong khi đó, ngưỡng SBS tăng rõ ràng được quan sát thấy với giá trị tối đa gấp 1,7 lần so với không có TFBG, giúp tăng công suất truyền hiệu dụng lên 33%. Tính ổn định hoạt động của phương pháp này cũng được xác nhận. Công trình này mở ra những cơ hội mới để ức chế SBS trong các hệ thống OFC, OFS và NLFL công suất cao."} +{"idx": 1870931, "en": "Silicon photonic integrated circuits (PICs) show great potential for many applications. The phase tuning technique is indispensable and of great importance in silicon PICs. An optical phase shifter with balanced overall performance on power consumption, insertion loss, footprint, and modulation bandwidth is essential for harnessing large-scale integrated photonics. However, few proposed phase shifter schemes on various platforms have achieved a well-balanced performance. In this Letter, we experimentally demonstrate a thermo-optic phase shifter based on a densely distributed silicon spiral waveguide on a silicon-on-insulator platform. The phase shifter shows a well-balanced performance in all aspects. The electrical power consumption is as low as 3 mW to achieve a π phase shift, the optical insertion loss is 0.9 dB per phase shifter, the footprint is 67×28µm2 under a standard silicon photonics fabrication process without silicon air trench or undercut process, and the modulation bandwidth is measured to be 39 kHz.", "gemini": "Các mạch tích hợp quang tử silicon (PIC) cho thấy tiềm năng lớn cho nhiều ứng dụng. Kỹ thuật điều chỉnh pha là không thể thiếu và có tầm quan trọng lớn trong các PIC silicon. Một bộ dịch pha quang với hiệu suất tổng thể cân bằng về mức tiêu thụ điện năng, tổn hao chèn, diện tích chiếm chỗ và băng thông điều chế là rất cần thiết để khai thác các thiết bị quang tử tích hợp quy mô lớn. Tuy nhiên, rất ít các lược đồ dịch pha được đề xuất trên nhiều nền tảng khác nhau đạt được hiệu suất cân bằng tốt. Trong Thư này, chúng tôi trình bày một bộ dịch pha nhiệt quang dựa trên một ống dẫn sóng xoắn ốc silicon phân bố dày đặc trên nền tảng silicon trên chất cách điện. Bộ dịch pha cho thấy hiệu suất cân bằng tốt ở mọi khía cạnh. Mức tiêu thụ điện năng chỉ 3 mW để đạt được độ dịch pha π, tổn hao chèn quang học là 0,9 dB trên mỗi bộ dịch pha, diện tích chiếm chỗ là 67 × 28µm2 theo quy trình chế tạo quang tử silicon tiêu chuẩn mà không cần rãnh khí silicon hoặc quy trình cắt, và băng thông điều chế được đo là 39 kHz."} +{"idx": 1870932, "en": "This erratum corrects the acronym 'ISW' as 'infinite square well'.", "gemini": "Bản đính chính này sửa lại từ viết tắt 'ISW' thành 'giếng vuông vô hạn'."} +{"idx": 1870933, "en": "Chromatin is the macromolecular assembly containing the cell's genetic information, and its architectural conformation facilitates accessibility to activation sites and thus gene expression. We have developed an analytical framework to quantify chromatin structure with spectral microscopy. Chromatin structure can be described as a mass fractal, with packing scaling D up to specific genomic length scales. Considering various system geometries, we established a model to measure D with the interferometric technique partial wave spectroscopy (PWS) and validated the analysis using finite difference time domain to simulate the PWS system. Calculations of D were consistent with ground truth electron microscopy measurements, enabling a high-throughput, label-free approach to quantifying chromatin structure in the nanometer length scale regime.", "gemini": "Chromatin là tập hợp đại phân tử chứa thông tin di truyền của tế bào và cấu trúc kiến trúc của nó tạo điều kiện thuận lợi cho khả năng tiếp cận các vị trí hoạt hóa và do đó biểu hiện gen. Chúng tôi đã phát triển một khuôn khổ phân tích để định lượng cấu trúc chromatin bằng kính hiển vi phổ. Cấu trúc chromatin có thể được mô tả như một phân số khối, với tỷ lệ đóng gói D lên đến các thang độ dài cụ thể của bộ gen. Xem xét nhiều hình dạng hệ thống khác nhau, chúng tôi đã thiết lập một mô hình để đo D bằng kỹ thuật giao thoa quang phổ sóng một phần (PWS) và xác thực phân tích bằng miền thời gian hữu hạn để mô phỏng hệ thống PWS. Các phép tính của D phù hợp với các phép đo hiển vi điện tử thực tế, cho phép tiếp cận không có nhãn, thông lượng cao để định lượng cấu trúc chromatin trong chế độ thang độ dài nanomet."} +{"idx": 1870934, "en": "We demonstrate theoretically and experimentally coherence-induced polarization changes in higher-order vector vortex beams (VVBs) with polarization singularity. The prominent depolarization on decreasing the transverse correlation width in a focused partially coherent VVB provides a means to shape the intensity profile and degree of polarization (DOP) while preserving the polarization distribution. The intensity variation and DOP dip are found to be dependent on the polarization singularity index of the beam. Our results may provide an additional degree of freedom in myriad applications presently projected with VVBs.", "gemini": "Chúng tôi chứng minh về mặt lý thuyết và thực nghiệm các thay đổi phân cực do tính kết hợp trong các chùm tia vectơ xoáy bậc cao (VVB) với kỳ dị phân cực. Sự khử phân cực nổi bật khi giảm chiều rộng tương quan ngang trong một VVB có tính kết hợp một phần tập trung cung cấp phương tiện để định hình cấu hình cường độ và mức độ phân cực (DOP) trong khi vẫn giữ nguyên phân bố phân cực. Biến thiên cường độ và độ trũng DOP được phát hiện phụ thuộc vào chỉ số kỳ dị phân cực của chùm tia. Kết quả của chúng tôi có thể cung cấp một mức độ tự do bổ sung trong vô số các ứng dụng hiện được chiếu với VVB."} +{"idx": 1870935, "en": "We experimentally demonstrate all-optical reconfigurable nonlinear activation functions in a cavity-loaded Mach-Zehnder interferometer device on a silicon photonics platform, via the free-carrier dispersion effect. Our device is programmable to generate various nonlinear activation functions, including sigmoid, radial-basis, clamped rectified linear unit, and softplus, with tunable thresholds. We simulate benchmark tasks such as XOR and MNIST handwritten digit classifications with experimentally measured activation functions and obtain accuracies of 100% and 94%, respectively. Our device can serve as nonlinear units in photonic neural networks, while its nonlinear transfer function can be flexibly programmed to optimize the performance of different neuromorphic tasks.", "gemini": "Chúng tôi chứng minh bằng thực nghiệm các hàm kích hoạt phi tuyến tính có thể cấu hình lại hoàn toàn quang học trong một thiết bị giao thoa kế Mach-Zehnder có tải khoang trên nền tảng photon học silicon, thông qua hiệu ứng phân tán của sóng mang tự do. Thiết bị của chúng tôi có thể lập trình để tạo ra nhiều hàm kích hoạt phi tuyến tính khác nhau, bao gồm sigmoid, cơ sở bán kính, đơn vị tuyến tính chỉnh lưu kẹp và softplus, với các ngưỡng có thể điều chỉnh. Chúng tôi mô phỏng các tác vụ chuẩn như phân loại chữ số viết tay XOR và MNIST với các hàm kích hoạt được đo bằng thực nghiệm và đạt độ chính xác lần lượt là 100% và 94%. Thiết bị của chúng tôi có thể đóng vai trò là các đơn vị phi tuyến tính trong mạng nơ-ron quang học, trong khi hàm truyền phi tuyến tính của nó có thể được lập trình linh hoạt để tối ưu hóa hiệu suất của các tác vụ hình thái thần kinh khác nhau."} +{"idx": 1870936, "en": "As an intrinsic feature of the optical field, chirality could induce many novel phenomena due to the interaction between chiral light and matter. Thus, the generation of optical fields possessing 2D or 3D chiral intensity patterns, called chiral intensity fields, has been widely studied. However, the control of chiral intensity field along the optical axis is still a challenge. Here, we propose a method to manipulate the axial propagation property of a focused chiral intensity field. Two modulation effects are realized: extended chiral intensity field with a focal depth >2λ at 90% mode correlation and tunable transformation of chirality during the axial propagation. This method is simple, stable, and easy to perform and therefore offers broad applications especially in optical tweezers and metamaterial fabrication.", "gemini": "Là một đặc điểm nội tại của trường quang học, tính trắc quang có thể gây ra nhiều hiện tượng mới lạ do sự tương tác giữa ánh sáng trắc quang và vật chất. Do đó, việc tạo ra các trường quang học sở hữu các mẫu cường độ trắc quang 2D hoặc 3D, được gọi là trường cường độ trắc quang, đã được nghiên cứu rộng rãi. Tuy nhiên, việc kiểm soát trường cường độ trắc quang dọc theo trục quang vẫn là một thách thức. Tại đây, chúng tôi đề xuất một phương pháp để thao tác thuộc tính truyền trục của trường cường độ trắc quang hội tụ. Hai hiệu ứng điều chế được thực hiện: trường cường độ trắc quang mở rộng với độ sâu tiêu cự >2λ ở độ tương quan chế độ 90% và sự biến đổi tính trắc quang có thể điều chỉnh trong quá trình truyền trục. Phương pháp này đơn giản, ổn định và dễ thực hiện, do đó mang lại nhiều ứng dụng rộng rãi, đặc biệt là trong nhíp quang và chế tạo vật liệu siêu vật liệu."} +{"idx": 1870937, "en": "In this Letter, we investigate the resolution of two-photon polymerization (2PP) with an amplified mode-locked external cavity diode laser with adjustable pulse length and a high repetition rate. The experimental results are analyzed with a newly developed 2PP model. Even with low pulse peak intensity, the produced structural dimensions are comparable to those generated by traditional 2PP laser sources. Thus, we show that a compact monolithic picosecond laser diode without amplification and with a repetition rate in the GHz regime can also be applied for 2PP. These results show the high application potential of compact mode-locked diode lasers for low-cost and compact 2PP systems.", "gemini": "Trong Thư này, chúng tôi nghiên cứu độ phân giải của quá trình trùng hợp hai photon (2PP) với laser diode khoang ngoài khóa chế độ được khuếch đại có độ dài xung có thể điều chỉnh và tốc độ lặp lại cao. Các kết quả thực nghiệm được phân tích bằng mô hình 2PP mới được phát triển. Ngay cả với cường độ đỉnh xung thấp, các kích thước cấu trúc tạo ra cũng tương đương với các kích thước được tạo ra bởi các nguồn laser 2PP truyền thống. Do đó, chúng tôi chỉ ra rằng một diode laser pico giây đơn khối nhỏ gọn không cần khuếch đại và có tốc độ lặp lại ở chế độ GHz cũng có thể được áp dụng cho 2PP. Những kết quả này cho thấy tiềm năng ứng dụng cao của laser diode khóa chế độ nhỏ gọn cho các hệ thống 2PP nhỏ gọn và chi phí thấp."} +{"idx": 1870938, "en": "An all-optical tunable filter based on a fiber Bragg grating (FBG) inscribed in a self-heated silica/silicone composite fiber is demonstrated. A thin silicone film is coated inside the suspended core fiber), which acts as the silicone cladding. A periodic refractive index modulation is inscribed in the silicone cladding by UV irradiation. Silicone is an organic material whose optical properties are different than silica, which leads to interesting applications. The high thermo-optic properties are studied and applied here. A 1550 nm pump laser is utilized to heat the silicone grating where a wavelength shift is observed for the gratings when subjected to different pump powers. Experimental results indicate a wavelength tuning coefficient of -0.128nm/mW with a response time of 0.5 s to obtain a wavelength shift of 1 nm under periodic pump light. The new design of this miniature all-optical filter is cost-effective and can potentially be adhered in optical fiber sensing and communication systems.", "gemini": "Một bộ lọc có thể điều chỉnh hoàn toàn quang dựa trên lưới nhiễu xạ sợi quang (FBG) được ghi trong sợi composite silica/silicone tự gia nhiệt được chứng minh. Một lớp silicone mỏng được phủ bên trong sợi lõi treo), đóng vai trò là lớp phủ silicone. Một sự điều chế chiết suất định kỳ được ghi vào lớp phủ silicone bằng cách chiếu tia UV. Silicone là một vật liệu hữu cơ có các tính chất quang học khác với silica, dẫn đến các ứng dụng thú vị. Các tính chất quang nhiệt cao được nghiên cứu và áp dụng tại đây. Một laser bơm 1550 nm được sử dụng để làm nóng lưới nhiễu xạ silicone, tại đó có thể quan sát thấy sự dịch chuyển bước sóng đối với các lưới nhiễu xạ khi chịu các công suất bơm khác nhau. Kết quả thực nghiệm chỉ ra hệ số điều chỉnh bước sóng là -0,128nm/mW với thời gian phản hồi là 0,5 giây để thu được độ dịch chuyển bước sóng là 1 nm dưới ánh sáng bơm định kỳ. Thiết kế mới của bộ lọc hoàn toàn quang thu nhỏ này có hiệu quả về chi phí và có khả năng được tuân thủ trong các hệ thống thông tin và cảm biến sợi quang."} +{"idx": 1870939, "en": "Real-time measurement of ultrafast pulses together with high temporal resolution and long recording length is an urgent requirement of all optical communication systems and nonlinear science. Here, external motion dynamics of soliton pairs in mode-locking ultrafast fiber lasers can be single-shot characterized with long recording length, by using an asynchronous four-wave-mixing (FWM)-based temporal magnifier (AFTM) system. Recording length of more than one thousand roundtrips can be achieved through the AFTM system. Temporal propagation dynamics of soliton pairs with tunable separations are observed, revealing that soliton pairs with narrower separation display vibration-like dynamics, while the two solitons with wider separation remain relatively unchanged. We believe our results will provide a promising solution for real-time measurement of ultrafast pulse and can offer novel insights for ultrafast transient dynamics in nonlinear optics.", "gemini": "Việc đo thời gian thực các xung cực nhanh cùng với độ phân giải thời gian cao và độ dài ghi dài là một yêu cầu cấp thiết của tất cả các hệ thống truyền thông quang học và khoa học phi tuyến. Ở đây, động học chuyển động bên ngoài của các cặp soliton trong laser sợi khóa chế độ cực nhanh có thể được đặc trưng bằng một lần chụp với độ dài ghi dài, bằng cách sử dụng hệ thống kính hiển vi thời gian dựa trên trộn bốn sóng không đồng bộ (FWM) (AFTM). Độ dài ghi hơn một nghìn vòng khứ hồi có thể đạt được thông qua hệ thống AFTM. Động học lan truyền thời gian của các cặp soliton với các khoảng cách có thể điều chỉnh được quan sát thấy, cho thấy rằng các cặp soliton với khoảng cách hẹp hơn hiển thị động lực giống như rung động, trong khi hai soliton với khoảng cách rộng hơn vẫn tương đối không thay đổi. Chúng tôi tin rằng kết quả của chúng tôi sẽ cung cấp một giải pháp đầy hứa hẹn cho phép đo thời gian thực xung cực nhanh và có thể cung cấp những hiểu biết mới về động lực học thoáng qua cực nhanh trong quang học phi tuyến."} +{"idx": 1870940, "en": "In this work, we demonstrate a novel high-power vertical-cavity surface-emitting laser (VCSEL) array with highly single-mode (SM) and single-polarized output performance without significantly increasing the intra-cavity loss and threshold current (Ith). By combining a low-loss zinc-diffusion aperture with an electroplated copper substrate, we can obtain a highly SM output (side mode suppression ratio >50dB) with a very narrow divergence angle (1/e2:∼10∘) under high output power (3.1 W; 1% duty cycle) and sustain a single polarization state, with a polarization suppression ratio of around 9 dB, under the full range of bias currents. Compared to the reference device without the copper substrate, the demonstrated array can not only switch the output optical spectra from quasi-SM to highly SM but also maintain a close threshold current value (Ith: 0.8 versus 0.7 mA per unit device) and slope efficiency. The enhancement in fundamental mode selectivity of our VCSEL structure can be attributed to the single-polarized lasing mode induced by tensile strain, which is caused by the electroplated copper substrate, as verified by the double-crystal x-ray measurement results.", "gemini": "Trong công trình này, chúng tôi trình bày một mạng laser phát xạ bề mặt khoang dọc công suất cao (VCSEL) mới có hiệu suất đầu ra cực đơn mode (SM) và cực đơn phân cực mà không làm tăng đáng kể tổn thất trong khoang và dòng ngưỡng (Ith). Bằng cách kết hợp một khẩu độ khuếch tán kẽm tổn thất thấp với một giá đỡ đồng mạ điện, chúng tôi có thể thu được đầu ra SM cao (tỷ lệ ức chế chế độ phụ >50dB) với góc phân kỳ rất hẹp (1/e2: ∼10∘) dưới công suất đầu ra cao (3,1 W; chu kỳ nhiệm vụ 1%) và duy trì trạng thái phân cực đơn, với tỷ lệ ức chế phân cực khoảng 9 dB, trong toàn dải dòng phân cực. So với thiết bị tham chiếu không có giá đỡ đồng, mảng đã chứng minh không chỉ có thể chuyển phổ quang đầu ra từ quasi-SM sang SM cao mà còn duy trì giá trị dòng ngưỡng gần (Ith: 0,8 so với 0,7 mA trên mỗi đơn vị thiết bị) và hiệu suất dốc. Sự gia tăng trong tính chọn lọc chế độ cơ bản của cấu trúc VCSEL của chúng tôi có thể được quy cho chế độ laser phân cực đơn do ứng suất kéo gây ra, do giá đỡ đồng mạ điện gây ra, như được xác minh bởi kết quả đo tia X tinh thể kép."} +{"idx": 1870941, "en": "Self-powered photodetectors have demonstrated potential for developing future wireless and implantable devices. Herein, we present a self-powered UV photodetector with an ultrahigh photoresponse based on vertically oriented and high crystalline quality n-type GaN nanorod arrays: poly(methyl methacrylate)/p-Si heterojunction. Benefiting from the highly efficient separation and transport of photoexcited electron-hole pairs, significant improvements in photoresponsivity are experimentally obtained. In a zero-biased self-powered detection mode, a 6.7AW-1 responsivity and 2.68×1013 Jones detectivity are achieved under 355 nm light illumination, and the response time is as low as 0.29/3.07 ms (rise/fall times).", "gemini": "Các máy dò ảnh tự cấp nguồn đã chứng minh tiềm năng để phát triển các thiết bị không dây và có thể cấy ghép trong tương lai. Trong bài báo này, chúng tôi trình bày một máy dò ảnh UV tự cấp nguồn với phản ứng quang cực cao dựa trên các mảng nanorod GaN loại n có chất lượng tinh thể cao và định hướng theo chiều dọc: dị hợp chất poly(methyl methacrylate)/p-Si. Nhờ sự phân tách và truyền hiệu quả cao của các cặp lỗ electron kích thích bằng quang, cải tiến đáng kể về độ nhạy quang đã thu được trong thực nghiệm. Trong chế độ phát hiện tự cấp nguồn không có độ lệch, độ nhạy 6,7AW-1 và khả năng phát hiện Jones 2,68×1013 đạt được dưới ánh sáng chiếu sáng 355 nm và thời gian phản hồi thấp tới 0,29/3,07 ms (thời gian tăng/giảm)."} +{"idx": 1870942, "en": "Due to the utilization of overlapped dipole resonances, traditional Huygens' metasurfaces suffer from dipole interactions. In this Letter, we propose a design of phase-gradient Huygens' metasurface based on the quadrupole resonances excited in the cross-shaped structures. The quadrupole resonances are theoretically shown insensitive to the quadrupole interactions. Benefiting from this intrinsic property, the proposed metasurface can well suppress element interaction influence and exhibits some impressive properties, including the ability to suppress high diffraction orders, tunable anomalous refractive angles, and high transmission efficiency. The numerical results show promising potential for quadrupole resonances to be applied in advanced Huygens' metasurface designs.", "gemini": "Do sử dụng các cộng hưởng lưỡng cực chồng chéo, các bề mặt siêu vật liệu Huygens truyền thống phải chịu tương tác lưỡng cực. Trong Thư này, chúng tôi đề xuất một thiết kế bề mặt siêu vật liệu Huygens có pha dốc dựa trên các cộng hưởng bốn cực được kích thích trong các cấu trúc hình chữ thập. Các cộng hưởng bốn cực về mặt lý thuyết cho thấy không nhạy cảm với các tương tác bốn cực. Được hưởng lợi từ đặc tính nội tại này, bề mặt siêu vật liệu được đề xuất có thể ức chế tốt ảnh hưởng của tương tác phần tử và thể hiện một số đặc tính ấn tượng, bao gồm khả năng ức chế các bậc nhiễu xạ cao, góc khúc xạ bất thường có thể điều chỉnh và hiệu suất truyền cao. Các kết quả số cho thấy tiềm năng đầy hứa hẹn của các cộng hưởng bốn cực để được áp dụng trong các thiết kế bề mặt siêu vật liệu Huygens tiên tiến."} +{"idx": 1870943, "en": "The combination of light sheet fluorescence microscopy (LSFM) and the optical clearing method can achieve fast three-dimensional high-resolution imaging. However, there is an essential contradiction between the field of view (FoV) and spatial resolution. Also, aberration and scattering still exist after tissue clearing, which seriously limits the imaging depth of LSFM. Here we propose a Schwartz modulation method and implement it in LSFM based on a quasi-Bessel beam to enlarge the imaging FoV without sacrificing its spatial resolution. The simulation results show that the FoV of the LSFM is enlarged by a factor of 1.73 compared to the Bessel beam. The capability of extremely fast decay along the optical axis makes Schwartz modulation more tolerant for scattering, indicating potential applications for deep tissue imaging. Also, the capability of sidelobe suppression effectively decreases unnecessary fluorescence excitation and photobleaching.", "gemini": "Sự kết hợp giữa kính hiển vi huỳnh quang tờ sáng (LSFM) và phương pháp làm trong quang học có thể đạt được hình ảnh ba chiều độ phân giải cao nhanh chóng. Tuy nhiên, có một mâu thuẫn thiết yếu giữa trường quan sát (FoV) và độ phân giải không gian. Ngoài ra, quang sai và tán xạ vẫn tồn tại sau khi làm trong mô, điều này làm hạn chế nghiêm trọng độ sâu hình ảnh của LSFM. Ở đây, chúng tôi đề xuất một phương pháp điều chế Schwartz và triển khai nó trong LSFM dựa trên chùm tia quasi-Bessel để mở rộng FoV hình ảnh mà không làm mất độ phân giải không gian của nó. Kết quả mô phỏng cho thấy FoV của LSFM được mở rộng lên 1,73 lần so với chùm tia Bessel. Khả năng phân rã cực nhanh dọc theo trục quang học làm cho điều chế Schwartz chịu được sự tán xạ nhiều hơn, cho thấy các ứng dụng tiềm năng cho hình ảnh mô sâu. Ngoài ra, khả năng ức chế thùy phụ hiệu quả làm giảm đáng kể sự kích thích huỳnh quang không cần thiết và sự tẩy trắng ảnh."} +{"idx": 1870944, "en": "We present a new, to the best of our knowledge, method to perform acousto-optic imaging based on a spatiotemporal structuration of long-duration acoustic plane waves. This approach is particularly relevant when using detectors with long integration times. We show how it is possible to reconstruct an image by measuring its two-dimensional Fourier components. A proof of concept is presented using a photorefractive detection scheme, demonstrating equal performances to direct imaging. The overall acquisition time is compatible with medical monitoring applications.", "gemini": "Chúng tôi trình bày một phương pháp mới, theo hiểu biết tốt nhất của chúng tôi, để thực hiện hình ảnh quang học dựa trên cấu trúc không gian thời gian của sóng phẳng âm thanh có thời lượng dài. Phương pháp này đặc biệt phù hợp khi sử dụng các máy dò có thời gian tích hợp dài. Chúng tôi chỉ ra cách có thể tái tạo hình ảnh bằng cách đo các thành phần Fourier hai chiều của nó. Một bằng chứng về khái niệm được trình bày bằng cách sử dụng một sơ đồ phát hiện quang khúc xạ, chứng minh hiệu suất bằng với hình ảnh trực tiếp. Tổng thời gian thu nhận tương thích với các ứng dụng theo dõi y tế."} +{"idx": 1870945, "en": "We develop a multimodal imaging platform, combining depth-resolved scattering contrast from spectral-domain optical coherence tomography (SD-OCT) with complementary, non-contact absorption contrast using photoacoustic remote sensing (PARS) microscopy. The system provides a widefield OCT mode using a telecentric scan lens, and a high-resolution, dual-contrast mode using a 0.26 numerical aperture apochromatic objective. An interlaced acquisition approach is used to achieve simultaneous, co-registered imaging. The SD-OCT modality provides a 9.7 µm axial resolution. Comprehensive in vivo imaging of a nude mouse ear is demonstrated, with the SD-OCT scattering intensity revealing dermal morphology, and PARS microscopy providing a map of microvasculature.", "gemini": "Chúng tôi phát triển một nền tảng hình ảnh đa phương thức, kết hợp độ tương phản tán xạ được giải quyết theo chiều sâu từ chụp cắt lớp quang học miền phổ (SD-OCT) với độ tương phản hấp thụ bổ sung, không tiếp xúc bằng kính hiển vi cảm biến từ xa quang âm (PARS). Hệ thống cung cấp chế độ OCT trường rộng bằng cách sử dụng ống kính quét telecentric và chế độ độ phân giải cao, độ tương phản kép bằng cách sử dụng vật kính vô sắc có độ mở số 0,26. Một phương pháp thu nhận xen kẽ được sử dụng để đạt được hình ảnh đồng thời, đồng đăng ký. Phương thức SD-OCT cung cấp độ phân giải trục 9,7 µm. Hình ảnh toàn diện trong cơ thể của tai chuột nhắt được chứng minh, với cường độ tán xạ SD-OCT cho thấy hình thái của lớp hạ bì và kính hiển vi PARS cung cấp bản đồ về hệ thống mạch máu nhỏ."} +{"idx": 1870946, "en": "Precision measurement of ro-vibrational transitions in the electronic ground state of the hydrogen molecule can be used to test quantum electrodynamics and also to determine the dimensionless proton-to-electron mass ratio. Saturation spectroscopy of the 2-0 overtone transitions of hydrogen deuterium (HD) were measured with three cavity-enhanced spectroscopy methods. With a sensitivity at the 10-13cm-1 level, we revealed a dispersion-like lineshape instead of a conventional Lamb \"dip,\" which explains the significant discrepancy among previous independent measurements. The spectra can be fit well by using the Fano profile. Centers of R(1) and R(3) lines were determined as 217 105 182 111 (19)(240)kHz and 220 704 305 234 (20)(240)kHz, respectively.", "gemini": "Phép đo chính xác các chuyển động quay rung trong trạng thái cơ bản của phân tử hydro có thể được sử dụng để kiểm tra điện động lực học lượng tử và cũng để xác định tỷ lệ khối lượng proton trên electron không có chiều. Phổ hấp thụ bão hòa của các chuyển động cộng hưởng 2-0 của hydro deuteri (HD) đã được đo bằng ba phương pháp phổ hấp thụ tăng cường khoang. Với độ nhạy ở mức 10-13cm-1, chúng tôi đã phát hiện ra một đường thẳng dạng phân tán thay vì \"điểm nhúng\" Lamb thông thường, điều này giải thích sự khác biệt đáng kể giữa các phép đo độc lập trước đó. Phổ có thể phù hợp tốt bằng cách sử dụng cấu hình Fano. Các tâm của các vạch R(1) và R(3) được xác định lần lượt là 217 105 182 111 (19)(240)kHz và 220 704 305 234 (20)(240)kHz."} +{"idx": 1870947, "en": "In endoscopic optical coherence tomography, a transparent protective sheath is used to protect the optics and tissue. However, the sheath causes astigmatism, which degrades transverse resolution and signal-to-noise ratio due to the cylindrical lens effect. Generally used methods for correcting this astigmatism are complex, difficult to control precisely, high-cost, and increase the dimensions of the imaging probe. To overcome these problems, we have developed an astigmatism-corrected imaging probe with an epoxy window. The astigmatism is precisely and cost-effectively adjusted controlling the curvature radius of the epoxy window, which is produced by soft lithography. Using the fiber optic fusion splicing, the fabrication process is simple. The fabricated imaging probe is almost monolithic, so its diameter is similar to that of a standard single-mode fiber. We demonstrate its astigmatism-correcting performance using focal spot analysis, imaging micro-beads and a biological sample.", "gemini": "Trong chụp cắt lớp quang học nội soi, một lớp vỏ bảo vệ trong suốt được sử dụng để bảo vệ quang học và mô. Tuy nhiên, lớp vỏ gây ra loạn thị, làm giảm độ phân giải ngang và tỷ lệ tín hiệu trên nhiễu do hiệu ứng thấu kính trụ. Các phương pháp thường được sử dụng để hiệu chỉnh loạn thị này rất phức tạp, khó kiểm soát chính xác, chi phí cao và làm tăng kích thước của đầu dò hình ảnh. Để khắc phục những vấn đề này, chúng tôi đã phát triển một đầu dò hình ảnh hiệu chỉnh loạn thị bằng cửa sổ epoxy. Loạn thị được điều chỉnh chính xác và hiệu quả về chi phí bằng cách kiểm soát bán kính cong của cửa sổ epoxy, được tạo ra bằng thạch bản mềm. Sử dụng ghép nối nóng chảy sợi quang, quá trình chế tạo rất đơn giản. Đầu dò hình ảnh được chế tạo gần như liền khối, vì vậy đường kính của nó tương tự như đường kính của sợi quang đơn mode tiêu chuẩn. Chúng tôi chứng minh hiệu suất hiệu chỉnh loạn thị của nó bằng cách phân tích điểm hội tụ, tạo ảnh các hạt vi và mẫu sinh học."} +{"idx": 1870948, "en": "The increasing interest in manipulating light on scales much smaller than its wavelength has driven intensive research on designing high efficiency optical antennas for near and far field applications. In particular, such nanoantennas serve as the main building block of metasurfaces, which were identified as an emerging technology for their capability in constructing versatile optical and electromagnetic devices. Hence, reducing the antennas' dimensions without compromising on their scattering efficiency is of utmost importance. In this Letter, we show that nanoantennas carved from hybrid plasmonic-dielectric waveguides preserve the unique properties of the hybrid modes, showing stronger confinement and better tunability at a relatively low loss, emanating from the coupling between the dielectric and plasmonic modes. This enables a design of high performance ultrasmall antennas that outperform dielectric and plasmonic nanoantennas at similar dimensions. We demonstrate this capability by simulating the performance of metasurfaces made of ultrasmall hybrid nanoantennas, proven to be superior over their dielectric and plasmonic counterparts. Using such hybrid nanoantennas as unit-cells in metasurfaces holds a great promise for designing new tunable, multifunctional, and low-loss nano-optical materials and applications.", "gemini": "Sự quan tâm ngày càng tăng đối với việc điều chế ánh sáng ở các thang độ nhỏ hơn nhiều so với bước sóng của nó đã thúc đẩy nghiên cứu chuyên sâu về thiết kế các ăng ten quang hiệu suất cao cho các ứng dụng trường gần và trường xa. Đặc biệt, các nanoăng ten như vậy đóng vai trò là khối xây dựng chính của bề mặt siêu vật liệu, được xác định là một công nghệ mới nổi vì khả năng chế tạo các thiết bị quang học và điện từ linh hoạt. Do đó, việc giảm kích thước của ăng ten mà không ảnh hưởng đến hiệu suất tán xạ của chúng là vô cùng quan trọng. Trong Thư này, chúng tôi chỉ ra rằng các nanoăng ten được khắc từ các ống dẫn sóng điện môi-plasmonic lai bảo toàn các đặc tính độc đáo của các chế độ lai, cho thấy sự hạn chế mạnh hơn và khả năng điều chỉnh tốt hơn ở mức tổn thất tương đối thấp, phát ra từ sự ghép nối giữa các chế độ điện môi và plasmonic. Điều này cho phép thiết kế các ăng ten cực nhỏ hiệu suất cao vượt trội hơn các ăng ten nano điện môi và plasmonic ở các kích thước tương tự. Chúng tôi chứng minh khả năng này bằng cách mô phỏng hiệu suất của các bề mặt siêu vật liệu được tạo thành từ các nanoăng ten lai cực nhỏ, đã được chứng minh là vượt trội hơn so với các đối tác điện môi và plasmonic của chúng. Việc sử dụng các nanoăng ten lai như vậy làm các ô đơn vị trong các bề mặt siêu vật liệu hứa hẹn rất lớn trong việc thiết kế các vật liệu và ứng dụng nano quang học đa chức năng, có thể điều chỉnh được và ít tổn hao mới."} +{"idx": 1870949, "en": "We demonstrate the fabrication of ultra-low-loss, all-fiber Fabry-Perot cavities that contain a nanofiber section, optimized for cavity quantum electrodynamics. By continuously monitoring the finesse and fiber radius during the fabrication of a nanofiber between two fiber Bragg gratings, we were able to precisely evaluate taper transmission as a function of radius. The resulting cavities have an internal round-trip loss of only 0.31% at a nanofiber waist radius of 207 nm, with a total finesse of 1380, and a maximum expected internal cooperativity of ∼1050 for a cesium atom on the nanofiber surface. Our ability to fabricate such high-finesse nanofiber cavities may open the door for the realization of high-fidelity scalable quantum networks.", "gemini": "Chúng tôi trình bày việc chế tạo các hốc Fabry-Perot toàn sợi có tổn hao cực thấp, chứa một phần nanofiber, được tối ưu hóa cho điện động lực học lượng tử hốc. Bằng cách liên tục theo dõi độ tinh tế và bán kính sợi trong quá trình chế tạo nanofiber giữa hai mạng nhiễu xạ sợi Bragg, chúng tôi có thể đánh giá chính xác độ truyền côn như một hàm của bán kính. Các hốc kết quả có tổn thất khứ hồi bên trong chỉ 0,31% ở bán kính eo nanofiber là 207 nm, với độ tinh tế tổng thể là 1380 và độ hợp tác bên trong tối đa dự kiến là ∼1050 đối với một nguyên tử cesium trên bề mặt nanofiber. Khả năng chế tạo các hốc nanofiber có độ tinh tế cao như vậy của chúng tôi có thể mở ra cánh cửa cho việc hiện thực hóa các mạng lượng tử có thể mở rộng độ trung thực cao."} +{"idx": 1870950, "en": "An ultrathin metal film with high transmittance and conductivity has been demonstrated to be a promising transparent electrode for organic light-emitting devices (OLEDs). However, mediocre surface morphology and continuity of evaporated metal films and the surface plasmon-polaritons (SPPs) energy loss between the metal electrode and organic layer still limit the external quantum efficiency (EQE) of OLEDs. Here, nanoimprint lithography has been directly applied on the ultrathin Au film with underlying uncured photopolymer to fabricate the nanopillared anode. Both the conductivity and transmittance of the nanopillared ultrathin Au film have been improved due to the improvement of continuity and surface smoothness. As we expected, the SPPs mode has been coupled into photons and further extracted from OLEDs by using the nanopillared Au film anode. Finally, 19.2% and 70.1% enhancement of current efficiency were achieved compared to the planar device with ultrathin Au anode and ITO anode, respectively.", "gemini": "Một lớp màng kim loại siêu mỏng có độ truyền dẫn và độ dẫn điện cao đã được chứng minh là một điện cực trong suốt đầy hứa hẹn cho các thiết bị phát sáng hữu cơ (OLED). Tuy nhiên, hình thái bề mặt tầm thường và tính liên tục của các lớp màng kim loại bay hơi và tổn thất năng lượng plasmon-polariton bề mặt (SPP) giữa điện cực kim loại và lớp hữu cơ vẫn hạn chế hiệu suất lượng tử bên ngoài (EQE) của OLED. Tại đây, kỹ thuật in thạch bản nano đã được áp dụng trực tiếp trên lớp Au siêu mỏng với lớp quang trùng hợp chưa đóng rắn bên dưới để chế tạo anot nanopillar. Độ dẫn điện và độ truyền dẫn của lớp Au siêu mỏng có nanopillar đều được cải thiện do cải thiện tính liên tục và độ mịn của bề mặt. Như chúng tôi mong đợi, chế độ SPP đã được ghép nối thành các photon và được chiết xuất thêm từ OLED bằng cách sử dụng anot màng Au nanopillar. Cuối cùng, hiệu suất dòng điện tăng 19,2% và 70,1% so với thiết bị phẳng có anot Au siêu mỏng và anot ITO."} +{"idx": 1870951, "en": "A novel probabilistic shaping (PS) scheme based on the quasi-Maxwell-Boltzmann (quasi-MB) distribution model is proposed in order to solve the incompatibility between PS and carrier phase recovery (CPR) algorithms, such as blind phase search (BPS) and principal component-based phase estimation (PCPE). In the proposed quasi-MB model, the same occurrence probability is assigned to each constellation point on the same square-ring, rather than on the same circle. Signals obeying the quasi-MB model have superior CPR friendliness compared to traditional PS signals. For a PS 64 quadrature amplitude modulation system, the simulation results indicate up to 51% and 21% normalized generalized mutual information (NGMI) improvements for PCPE and BPS, respectively. Experimental verification of the proposed quasi-MB scheme was demonstrated in a 10 Gbaud coherent detection system. The results show that when the optical signal-to-noise ratio (OSNR) is low, the quasi-MB model can help the BPS algorithm to achieve better NGMI performance and, when the OSNR is high, the proposed model can also solve the incompatibility between the PCPE algorithm and PS.", "gemini": "Một lược đồ định hình xác suất (PS) mới dựa trên mô hình phân phối quasi-Maxwell-Boltzmann (quasi-MB) được đề xuất để giải quyết sự không tương thích giữa các thuật toán PS và khôi phục pha sóng mang (CPR), chẳng hạn như tìm kiếm pha mù (BPS) và ước tính pha dựa trên thành phần chính (PCPE). Trong mô hình quasi-MB được đề xuất, cùng một xác suất xảy ra được gán cho mỗi điểm chòm sao trên cùng một vòng tròn vuông, chứ không phải trên cùng một vòng tròn. Các tín hiệu tuân theo mô hình quasi-MB có khả năng tương thích CPR vượt trội so với các tín hiệu PS truyền thống. Đối với hệ thống điều chế biên độ vuông góc PS 64, kết quả mô phỏng chỉ ra cải thiện thông tin chung chuẩn hóa (NGMI) lên tới 51% và 21% cho PCPE và BPS. Việc xác minh thực nghiệm lược đồ quasi-MB được đề xuất đã được chứng minh trong hệ thống phát hiện liên hợp 10 Gbaud. Kết quả cho thấy khi tỷ lệ tín hiệu trên nhiễu quang (OSNR) thấp, mô hình quasi-MB có thể giúp thuật toán BPS đạt được hiệu suất NGMI tốt hơn và khi OSNR cao, mô hình được đề xuất cũng có thể giải quyết được sự không tương thích giữa thuật toán PCPE và PS."} +{"idx": 1870952, "en": "This work experimentally investigates the impact of p-doping on the relative intensity noise (RIN) properties and subsequently on the modulation properties of semiconductor quantum dot (QD) lasers epitaxially grown on silicon. Owing to the low threading dislocation density and the p-modulation doped GaAs barrier layer in the active region, the RIN level is found very stable with temperature with a minimum value of -150dB/Hz. The dynamical features extracted from the RIN spectra show that p-doping between zero and 20 holes/dot strongly modifies the modulation properties and gain nonlinearities through increased internal losses in the active region and thereby hinders the maximum achievable bandwidth. Overall, this Letter is important for designing future high-speed and low-noise QD devices integrated in future photonic integrated circuits.", "gemini": "Nghiên cứu này điều tra thực nghiệm tác động của việc p-doping lên các đặc tính nhiễu cường độ tương đối (RIN) và sau đó là các đặc tính điều chế của laser chấm lượng tử bán dẫn (QD) phát triển ngoài tử trên silicon. Do mật độ sai lệch luồn thấp và lớp chắn GaAs được p-doping trong vùng hoạt động, mức RIN được phát hiện rất ổn định với nhiệt độ với giá trị tối thiểu là -150dB/Hz. Các đặc điểm động được trích xuất từ ​​phổ RIN cho thấy p-doping trong khoảng từ 0 đến 20 lỗ/chấm làm thay đổi mạnh các đặc tính điều chế và độ không tuyến tính của độ lợi thông qua tổn thất bên trong tăng trong vùng hoạt động và do đó cản trở băng thông tối đa có thể đạt được. Nhìn chung, Bức thư này rất quan trọng để thiết kế các thiết bị QD tốc độ cao và tiếng ồn thấp trong tương lai được tích hợp trong các mạch tích hợp quang tử trong tương lai."} +{"idx": 1870953, "en": "The wideband tuning of strong bands generated through polarization modulation instability (PMI) in microstructured optical fibers (MOFs) is reported. Tunability is achieved by exploiting the dependence of the phase-matching condition on the fiber's chromatic dispersion and birefringence, which is particularly sensitive when the fiber is pumped near the zero-dispersion wavelength. MOFs designed to accomplish PMI phase-matching when they are infiltrated with ethanol and pumped at 1064 nm were designed and fabricated. Taking advantage of the large thermo-optic coefficient of ethanol, both chromatic dispersion and birefringence were varied through temperature. Wavelength shifts from 937 nm to 863 nm (anti-Stokes) and from 1231 nm to 1387 nm (Stokes) are demonstrated. Such a tuning range corresponds to a frequency shift between the pump and a PMI sideband ranging from 1274cm-1 to 2189cm-1.", "gemini": "Việc điều chỉnh băng thông rộng của các băng mạnh được tạo ra thông qua bất ổn điều chế phân cực (PMI) trong các sợi quang có cấu trúc vi mô (MOF) đã được báo cáo. Khả năng điều chỉnh được đạt được bằng cách khai thác sự phụ thuộc của điều kiện khớp pha vào độ tán sắc màu và lưỡng chiết của sợi, điều này đặc biệt nhạy cảm khi sợi được bơm gần bước sóng không có độ tán sắc. Các MOF được thiết kế để thực hiện khớp pha PMI khi chúng được thấm ethanol và được bơm ở bước sóng 1064 nm đã được thiết kế và chế tạo. Tận dụng hệ số quang nhiệt lớn của ethanol, cả độ tán sắc màu và lưỡng chiết đều thay đổi theo nhiệt độ. Các dịch chuyển bước sóng từ 937 nm đến 863 nm (chống Stokes) và từ 1231 nm đến 1387 nm (Stokes) đã được chứng minh. Phạm vi điều chỉnh như vậy tương ứng với dịch chuyển tần số giữa bơm và dải bên PMI trong khoảng từ 1274cm-1 đến 2189cm-1."} +{"idx": 1870954, "en": "In this Letter, we report the experimental observations of a tunable curved photonic nanojet (photonic hook) generated by a 5 µm polydimethylsiloxane microcylinder deposited on a silicon substrate and illuminated by 405 nm laser beam. A moveable opaque aluminum-mask is mounted in front of the microcylinder implementing partial illumination and imparting spatial curvature to the photonic nanojet. Experimental results of main parameters (tilt angle, width, and intensity) of emerging photonic hooks exhibit close agreement with numerical predictions of the near-field optical structures. The experimentally measured full widths at half-maximum of photonic hooks are 0.48λ, 0.56λ, and 0.76λ for tilt angles of θ=0∘, 5.7°, and 20.1°, respectively. Photonic hooks possess great potential in complex manipulation such as super-resolution imaging, surface fabrication, and optomechanical manipulation along curved trajectories.", "gemini": "Trong Thư này, chúng tôi báo cáo các quan sát thực nghiệm về một tia nano quang cong có thể điều chỉnh (móc quang) được tạo ra bởi một vi trụ polydimethylsiloxane 5 µm lắng đọng trên một chất nền silicon và được chiếu sáng bằng chùm tia laser 405 nm. Một mặt nạ nhôm đục có thể di chuyển được gắn ở phía trước vi trụ để thực hiện chiếu sáng một phần và tạo độ cong không gian cho tia nano quang. Kết quả thực nghiệm của các thông số chính (góc nghiêng, chiều rộng và cường độ) của các móc quang mới nổi cho thấy sự phù hợp chặt chẽ với các dự đoán số về các cấu trúc quang học trường gần. Chiều rộng toàn bộ được đo bằng thực nghiệm ở nửa cực đại của các móc quang là 0,48λ, 0,56λ và 0,76λ đối với các góc nghiêng θ = 0∘, 5,7° và 20,1°, tương ứng. Các móc quang có tiềm năng lớn trong thao tác phức tạp như hình ảnh siêu phân giải, chế tạo bề mặt và thao tác quang cơ dọc theo các quỹ đạo cong."} +{"idx": 1870955, "en": "We report on the formation of novel turbulent coherent structures in a long cavity semiconductor laser near the lasing threshold. Experimentally, the laser emits a series of power dropouts within a roundtrip, and the number of dropouts per series depends on a set of parameters including the bias current. At fixed parameters, the drops remain dynamically stable, repeating over many roundtrips. By reconstructing the laser electric field in the case where the laser emits one dropout per roundtrip and simulating its dynamics using a time-delayed model, we discuss the reasons for long-term sustainability of these solutions. We suggest that the observed dropouts are closely related to the coherent structures of the cubic complex Ginzburg-Landau equation.", "gemini": "Chúng tôi báo cáo về sự hình thành các cấu trúc liên kết hỗn loạn mới lạ trong một laser bán dẫn khoang dài gần ngưỡng phát sáng. Về mặt thực nghiệm, laser phát ra một loạt các sự cố mất điện trong một chuyến đi khứ hồi và số lần mất điện trên mỗi loạt phụ thuộc vào một tập hợp các thông số bao gồm cả dòng điện phân cực. Ở các thông số cố định, các giọt vẫn ổn định động, lặp lại sau nhiều chuyến đi khứ hồi. Bằng cách tái tạo trường điện laser trong trường hợp laser phát ra một lần mất điện trên mỗi chuyến đi khứ hồi và mô phỏng động lực của nó bằng mô hình trễ thời gian, chúng tôi thảo luận về các lý do khiến các giải pháp này có thể duy trì lâu dài. Chúng tôi cho rằng các sự cố mất điện được quan sát thấy có liên quan chặt chẽ đến các cấu trúc liên kết của phương trình Ginzburg-Landau phức tạp khối."} +{"idx": 1870956, "en": "A high degree of chaos synchronization among transverse mode pairs with parity-symmetric polarizations was demonstrated in a laser-diode pumped thin-slice c-cut Nd:GdVO4 laser. The amplitude correlation coefficient greater than R>0.994 resulted from a non-reversing mirror symmetric-polarization-dependent structural change in the modal patterns, where chaotic dynamics of the whole system consisting of qualitatively different dynamics, which depend on the polarization crossing angle, resembled that of a chaotic single mode laser. A self-organized sender-receiver type of chaos synchronization of a single pair of modes among an infinite number of parity-symmetric polarizations, the associated chaotic camouflaging, and the extracting experiment of a self-mixing solid-state laser Doppler velocimetry signal were demonstrated.", "gemini": "Một mức độ đồng bộ hỗn loạn cao giữa các cặp chế độ ngang với phân cực đối xứng chẵn lẻ đã được chứng minh trong một laser Nd:GdVO4 cắt c lát mỏng được bơm bằng diode laser. Hệ số tương quan biên độ lớn hơn R> 0,994 là kết quả của sự thay đổi cấu trúc phụ thuộc phân cực đối xứng gương không đảo ngược trong các mẫu chế độ, trong đó động lực hỗn loạn của toàn bộ hệ thống bao gồm các động lực khác nhau về mặt định tính, phụ thuộc vào góc giao nhau của phân cực, giống với động lực của một laser chế độ đơn hỗn loạn. Một loại đồng bộ hỗn loạn kiểu người gửi-người nhận tự tổ chức của một cặp chế độ duy nhất trong số vô số các phân cực đối xứng chẵn lẻ, ngụy trang hỗn loạn liên quan và thí nghiệm trích xuất tín hiệu đo vận tốc Doppler laser trạng thái rắn tự trộn đã được chứng minh."} +{"idx": 1870957, "en": "We demonstrated an ultra-low-noise polarization-maintaining (PM) single-frequency fiber laser at 2 µm. Both relative intensity noise (RIN) and frequency noise were improved by suppressing the pump source RIN using feedback control. After a two-stage Tm3+-doped PM fiber amplifier, the output power reached about 5 W, and the amplifier did not introduce any observable extra frequency noise. The frequency noise was less than 100Hz/Hz above 13 Hz, which is comparable to the frequency noise of a typical seed laser of the Advanced LIGO high-power laser. The central wavelength was measured to be 1990.25 nm, with a polarization extinction ratio above 24 dB.", "gemini": "Chúng tôi đã chứng minh một tia laser sợi duy trì phân cực (PM) có độ nhiễu cực thấp ở 2 µm. Cả độ nhiễu cường độ tương đối (RIN) và độ nhiễu tần số đều được cải thiện bằng cách ức chế RIN của nguồn bơm sử dụng điều khiển phản hồi. Sau bộ khuếch đại sợi PM pha tạp Tm3+ hai tầng, công suất đầu ra đạt khoảng 5 W và bộ khuếch đại không đưa vào bất kỳ độ nhiễu tần số nào có thể quan sát được. Độ nhiễu tần số nhỏ hơn 100Hz/Hz trên 13 Hz, tương đương với độ nhiễu tần số của tia laser hạt giống thông thường của tia laser công suất cao Advanced LIGO. Bước sóng trung tâm được đo là 1990,25 nm, với tỷ lệ tiêu quang phân cực trên 24 dB."} +{"idx": 1870958, "en": "TE/TM-pass polarizers based on the lithium niobate-silicon nitride hybrid platform are numerically proposed for the first time, to the best of our knowledge. By utilizing the lateral leakage of a shallowly etched rib waveguide, 1-mm-long TE/TM-pass polarizers with high extinction ratios of 28.72/24.03 dB are obtained. Because of the anisotropy of the lithium niobate, the lateral leakage of TE/TM polarization modes can occur along crystallographic z/y directions, respectively. Such TE/TM-pass polarizers can be integrated in the same wafer.", "gemini": "Theo hiểu biết của chúng tôi, lần đầu tiên các phân cực thông TE/TM dựa trên nền tảng lai nitrua silic-lithi niobat được đề xuất theo phương pháp số. Bằng cách sử dụng rò rỉ bên của ống dẫn sóng gân cạn được khắc nông, người ta thu được các phân cực thông TE/TM dài 1 mm với tỷ lệ tiêu quang cao là 28,72/24,03 dB. Do tính dị hướng của lithi niobat, rò rỉ bên của các chế độ phân cực TE/TM có thể xảy ra theo các hướng tinh thể z/y, tương ứng. Các phân cực thông TE/TM như vậy có thể được tích hợp trong cùng một tấm wafer."} +{"idx": 1870959, "en": "Recent progress has enabled the reconstruction of the local (i.e., depth-resolved) optic axis (OAx) of biological tissue from measurements made with polarization-sensitive optical coherence tomography (PS-OCT). Here we demonstrate local OAx imaging in healthy human skin in vivo. The images reveal dense, weaving patterns that are imperceptible in OCT intensity tomograms or conventional PS-OCT metrics and that suggest a mesh-like tissue organization, consistent with the morphology of dermal collagen. Using co-registered polarization-sensitive optical coherence microscopy, we furthermore investigated the impact of spatial resolution on the recovered OAx patterns and confirmed their consistency. OAx orientation as a contrast mechanism merits further exploration for applications in dermatology.", "gemini": "Những tiến bộ gần đây đã cho phép tái tạo trục quang học cục bộ (tức là độ sâu được giải quyết) (OAx) của mô sinh học từ các phép đo được thực hiện bằng phương pháp chụp cắt lớp quang học phân cực nhạy (PS-OCT). Ở đây, chúng tôi trình bày hình ảnh OAx cục bộ trên da người khỏe mạnh trong cơ thể sống. Các hình ảnh cho thấy các mẫu dệt dày đặc, không thể nhận thấy trong cắt lớp quang học cường độ OCT hoặc các số liệu PS-OCT thông thường và cho thấy tổ chức mô giống như lưới, phù hợp với hình thái của collagen da. Sử dụng kính hiển vi quang học phân cực nhạy đồng đăng ký, chúng tôi tiếp tục nghiên cứu tác động của độ phân giải không gian lên các mẫu OAx phục hồi và xác nhận tính nhất quán của chúng. Định hướng OAx như một cơ chế tương phản đáng được khám phá thêm cho các ứng dụng trong da liễu."} +{"idx": 1870960, "en": "We experimentally study the optical properties of Al-SiO2 hyperbolic metamaterials. The hyperbolic dispersion and ultrathin Al film ∼12nm make the Al-SiO2 multilayers possess transmission efficiency ∼0.3 for the spatial frequency ranging from k0 to 1.42 k0 at 363.8 nm illumination. The atomic concentration of 3% Cu-doping is experimentally demonstrated to obtain Al film ∼12nm with root-mean-square roughness ∼0.49nm. The fabricated Al-SiO2 multilayers combined with the optimized ZrO2 resonant grating with period 280 nm serves as a structured illumination device, which efficiently converts the P-polarized normal field to the spatial frequency 1.3 k0 structured field. The measured average transmission intensity of the ±1 order is ∼0.14, and the intensity ratio of the ±1 order and 0 order is ∼65. This Letter is promising for structured illumination, spontaneous emission enhancement, Cherenkov radiation, etc.", "gemini": "Chúng tôi nghiên cứu thực nghiệm các đặc tính quang học của siêu vật liệu hypebol Al-SiO2. Độ tán sắc hypebol và lớp màng Al siêu mỏng ∼12nm làm cho các lớp phủ đa lớp Al-SiO2 có hiệu suất truyền dẫn ∼0,3 cho tần số không gian trong khoảng từ k0 đến 1,42 k0 ở độ chiếu sáng 363,8 nm. Nồng độ nguyên tử của Cu-pha tạp 3% được chứng minh thực nghiệm để thu được lớp màng Al ∼12nm với độ nhám căn bậc hai trung bình ∼0,49nm. Các lớp phủ đa lớp Al-SiO2 chế tạo kết hợp với mạng nhiễu xạ cộng hưởng ZrO2 được tối ưu hóa với chu kỳ 280 nm đóng vai trò như một thiết bị chiếu sáng có cấu trúc, giúp chuyển đổi hiệu quả trường bình thường phân cực P sang trường có cấu trúc tần số không gian 1,3 k0. Cường độ truyền trung bình được đo của bậc ±1 là ∼0,14 và tỷ số cường độ của bậc ±1 và bậc 0 là ∼65. Bức thư này hứa hẹn cho việc chiếu sáng có cấu trúc, tăng cường phát xạ tự phát, bức xạ Cherenkov, v.v."} +{"idx": 1870961, "en": "In this Letter, we propose and demonstrate a practical optical-spatial-summing-based non-orthogonal multiple access (OSS-NOMA) technique for visible light communication (VLC) systems. This technique is innovative in adopting OSS in that the transmitter of OSS-NOMA VLC can be built upon commercial illuminating light emitting diodes (LEDs), free of LEDs' harmful nonlinearity. Unlike conventional NOMA VLC using analog components such as digital-to-analog converters and bias-T in the transmitter side, OSS-NOMA exploits only digital control signals to drive a LED array in forming optical power superposition for NOMA signals. We demonstrate that by simply switching different amounts of LED chips on or off, the proposed OSS-NOMA transmitter can deliver a fine-grained power allocation ratio ranging from 0.01 to one for two users. The implemented OSS-NOMA VLC prototype leveraging commercial components can achieve low bit error rates of ≤3.1×10-3 for two users at a data rate of 800 kbps, confirming the promising potential of this novel OSS-NOMA VLC for Internet of Things (IoT) applications.", "gemini": "Trong bức thư này, chúng tôi đề xuất và trình bày một kỹ thuật truy cập nhiều không trực giao dựa trên tổng hợp không gian quang (OSS-NOMA) thực tế cho các hệ thống truyền thông ánh sáng khả kiến (VLC). Kỹ thuật này có tính sáng tạo khi áp dụng OSS ở chỗ bộ phát của OSS-NOMA VLC có thể được xây dựng dựa trên các điốt phát sáng chiếu sáng thương mại (LED), không có tính phi tuyến có hại của LED. Không giống như NOMA VLC thông thường sử dụng các thành phần analog như bộ chuyển đổi số sang analog và bias-T ở phía bộ phát, OSS-NOMA chỉ sử dụng các tín hiệu điều khiển kỹ thuật số để điều khiển một mảng LED trong việc tạo chồng chập công suất quang cho các tín hiệu NOMA. Chúng tôi chứng minh rằng chỉ bằng cách bật hoặc tắt các lượng chip LED khác nhau, bộ phát OSS-NOMA được đề xuất có thể cung cấp tỷ lệ phân bổ công suất chi tiết trong khoảng từ 0,01 đến một cho hai người dùng. Nguyên mẫu OSS-NOMA VLC được triển khai tận dụng các thành phần thương mại có thể đạt được tỷ lệ lỗi bit thấp ≤3,1×10-3 cho hai người dùng ở tốc độ dữ liệu 800 kbps, xác nhận tiềm năng đầy hứa hẹn của OSS-NOMA VLC mới này cho các ứng dụng Internet of Things (IoT)."} +{"idx": 1870962, "en": "In this Letter, we propose a compact multimode fiber endoscope which employs wavefront shaping with a digital micromirror device (DMD). An automated single calibration step allows us to correct for optical misalignment, and the method achieves accurate focusing at various depths in the sample through rapid switching of holographic patterns by the DMD. The speed of calibration is one or two orders of magnitude faster than existing methods. The method, single calibration multimode fiber imaging (SCMFI), is compared with existing methods, and its performance is validated. We show a near diffraction limited focusing capability at imaging depths up to 110 µm with near constant lateral resolutions of 1.4 µm. Finally, we demonstrate the method for the imaging of small fluorescent beads embedded in a 3D matrix. The results indicate excellent power penetration and focusing performance. Combined with the high speed of SCMFI, this paves the way for volumetric tissue endoscopy at depth.", "gemini": "Trong bức thư này, chúng tôi đề xuất một nội soi sợi đa chế độ nhỏ gọn sử dụng tạo hình mặt sóng với thiết bị vi gương kỹ thuật số (DMD). Một bước hiệu chuẩn đơn tự động cho phép chúng tôi hiệu chỉnh sai lệch quang học và phương pháp này đạt được khả năng lấy nét chính xác ở nhiều độ sâu khác nhau trong mẫu thông qua việc chuyển đổi nhanh các mẫu toàn ký bằng DMD. Tốc độ hiệu chuẩn nhanh hơn các phương pháp hiện có từ một đến hai bậc độ lớn. Phương pháp này, hình ảnh sợi đa chế độ hiệu chuẩn đơn (SCMFI), được so sánh với các phương pháp hiện có và hiệu suất của nó được xác nhận. Chúng tôi trình bày khả năng lấy nét gần như giới hạn nhiễu xạ ở độ sâu hình ảnh lên đến 110 µm với độ phân giải ngang gần như không đổi là 1,4 µm. Cuối cùng, chúng tôi trình bày phương pháp để chụp ảnh các hạt huỳnh quang nhỏ được nhúng trong ma trận 3D. Kết quả cho thấy khả năng thâm nhập và lấy nét tuyệt vời. Kết hợp với tốc độ cao của SCMFI, điều này mở đường cho nội soi mô thể tích ở độ sâu."} +{"idx": 1870963, "en": "High-purity silicon is a readily available material of utility in realizing a variety of long-wavelength optical and guided wave components. The transmittance of uncompensated for silicon is measured in the far- and mid-infrared regimes at room and cryogenic temperatures. The experimental and analysis techniques used to extract the refractive index from 100-1000cm-1 (100-10 µm) are presented, and the results are compared to the literature. An average refractive index below 300cm-1, n^(300K)=3.417+i8.9×10-5, which transitions in cooling to n^(10K)=3.389+i4.9×10-6, is observed.", "gemini": "Silicon có độ tinh khiết cao là vật liệu dễ kiếm có tiện ích trong việc nhận ra nhiều thành phần sóng dẫn hướng và quang học bước sóng dài. Độ truyền qua của silicon không được bù đắp được đo ở chế độ hồng ngoại xa và trung bình ở nhiệt độ phòng và nhiệt độ lạnh. Các kỹ thuật phân tích và thử nghiệm được sử dụng để trích xuất chỉ số khúc xạ từ 100-1000cm-1 (100-10 µm) được trình bày và các kết quả được so sánh với tài liệu. Một chỉ số khúc xạ trung bình dưới 300cm-1, n^(300K)=3.417+i8.9×10-5, chuyển đổi khi làm mát thành n^(10K)=3.389+i4.9×10-6, được quan sát."} +{"idx": 1870964, "en": "This publisher's note contains corrections to Opt. Lett.44, 4737 (2019) OPLEDP0146-959210.1364/OL.44.004737.", "gemini": "Ghi chú của nhà xuất bản này có chứa các bản sửa lỗi cho Opt. Lett.44, 4737 (2019) OPLEDP0146-959210.1364/OL.44.004737."} +{"idx": 1870965, "en": "We demonstrate a technique to compress spectral information in dual-comb spectroscopy that relies on subsampling of the electrical interferogram. It enables us to reduce the data sample rate by arbitrary factors directly in the sampling process or in post-processing of existing data. A demonstration code is provided.", "gemini": "Chúng tôi trình bày một kỹ thuật để nén thông tin quang phổ trong quang phổ lược kép dựa trên việc lấy mẫu phụ của giao thoa kế điện. Nó cho phép chúng tôi giảm tốc độ lấy mẫu dữ liệu theo các hệ số tùy ý trực tiếp trong quá trình lấy mẫu hoặc trong quá trình xử lý dữ liệu hiện có. Một mã trình diễn được cung cấp."} +{"idx": 1870966, "en": "Non-confocal adaptive optics scanning laser ophthalmoscopy (AOSLO) has enhanced the study of human retinal photoreceptors by providing complementary information to standard confocal AOSLO images. Previously we developed the first confocal handheld AOSLO (HAOSLO) capable of in vivo cone photoreceptor imaging in supine and non-cooperative patients. Here, we introduce the first multimodal (M-)HAOSLO for confocal and non-confocal split-detection (SD) imaging to allow for more comprehensive patient data collection. Aside from its unprecedented miniature size and weight, M-HAOSLO is also the first system to perform sensorless wavefront-corrected SD imaging of cone photoreceptors.", "gemini": "Kỹ thuật quét hình ảnh võng mạc bằng kính hiển vi quét laser thích ứng không đồng tiêu (AOSLO) đã tăng cường nghiên cứu về tế bào thụ quang võng mạc ở người bằng cách cung cấp thông tin bổ sung cho hình ảnh AOSLO đồng tiêu chuẩn. Trước đây, chúng tôi đã phát triển AOSLO cầm tay đồng tiêu đầu tiên (HAOSLO) có khả năng chụp ảnh tế bào thụ quang hình nón in vivo ở bệnh nhân nằm ngửa và không hợp tác. Tại đây, chúng tôi giới thiệu HAOSLO đa phương thức (M-) đầu tiên để chụp ảnh phát hiện phân tách (SD) đồng tiêu và không đồng tiêu để cho phép thu thập dữ liệu bệnh nhân toàn diện hơn. Bên cạnh kích thước và trọng lượng nhỏ chưa từng có, M-HAOSLO cũng là hệ thống đầu tiên thực hiện chụp ảnh SD hiệu chỉnh mặt sóng không cảm biến của tế bào thụ quang hình nón."} +{"idx": 1870967, "en": "Resonant frequency doubling of periodically phase-modulated single-frequency fiber laser is investigated as a method for power scaling of visible fiber lasers. Sinusoidal phase modulation is applied to generate few-frequency lasers at 1064 nm in the proof of principle experiments. By adjusting the modulation frequency to match the free spectral range of a doubling cavity, a resonant enhancement condition can be achieved and a near 30 W 532 nm laser is generated with a maximum conversion efficiency above 80%. The indistinguishable conversion efficiencies between the single-frequency and few-frequency cases prove the feasibility of the approach. Interesting spectral evolvement of the phase-modulated laser in second-harmonic generation is analyzed theoretically and observed in the experiment.", "gemini": "Tần số cộng hưởng tăng gấp đôi của laser sợi đơn tần điều chế pha theo chu kỳ được nghiên cứu như một phương pháp tăng công suất của laser sợi khả kiến. Điều chế pha hình sin được áp dụng để tạo ra laser tần số thấp ở bước sóng 1064 nm trong các thí nghiệm chứng minh nguyên lý. Bằng cách điều chỉnh tần số điều chế để phù hợp với dải quang phổ tự do của khoang nhân đôi, có thể đạt được điều kiện tăng cường cộng hưởng và tạo ra laser 532 nm gần 30 W với hiệu suất chuyển đổi tối đa trên 80%. Hiệu suất chuyển đổi không thể phân biệt giữa các trường hợp tần số đơn và tần số thấp chứng minh tính khả thi của phương pháp tiếp cận. Sự tiến hóa quang phổ thú vị của laser điều chế pha trong quá trình tạo sóng hài bậc hai được phân tích về mặt lý thuyết và quan sát trong thí nghiệm."} +{"idx": 1870968, "en": "Modulation transfer spectroscopy is used to demonstrate absolute frequency stabilization of an 8.6-µm-wavelength quantum cascade laser against a sub-Doppler absorption of the CHF3 molecule. The obtained spectral emission properties are thoroughly characterized through a self-referenced optical frequency comb, stabilized against either a GPS-disciplined Rb clock or a 1.54-µm Er-fiber laser locked to a high-finesse ultra-low-expansion optical cavity. Fractional long-term stability and accuracy at a level of 4×10-12 (at 100 s) and 3×10-10, respectively, are demonstrated, along with an emission linewidth as narrow as 10 kHz for observation times of 0.1 s.", "gemini": "Phổ hấp thụ điều chế được sử dụng để chứng minh sự ổn định tần số tuyệt đối của laser thác lượng tử bước sóng 8,6 µm so với sự hấp thụ dưới Doppler của phân tử CHF3. Các đặc tính phát xạ quang phổ thu được được đặc trưng kỹ lưỡng thông qua lược tần số quang học tự tham chiếu, được ổn định so với đồng hồ Rb được điều chỉnh bằng GPS hoặc laser sợi Er 1,54 µm được khóa vào khoang quang học có độ giãn nở cực thấp, độ tinh tế cao. Độ ổn định và độ chính xác lâu dài theo từng phần ở mức 4 × 10-12 (ở 100 giây) và 3 × 10-10, tương ứng, được chứng minh, cùng với độ rộng vạch phát xạ hẹp tới 10 kHz đối với thời gian quan sát là 0,1 giây."} +{"idx": 1870969, "en": "We present a scheme to realize precise discrimination of chiral molecules in a cavity. Assisted by additional laser pulses, cavity fields can evolve into different coherence states with contrary-sign displacements according to the handedness of molecules. Consequently, the handedness of molecules can be read out with homodyne measurement on the cavity, and the successful probability is nearly unity without very strong cavity fields. Numerical results show that the scheme is insensitive to errors, noise, and decoherence. Therefore, the scheme may provide helpful perspectives for accurate discrimination of chiral molecules.", "gemini": "Chúng tôi trình bày một lược đồ để nhận ra sự phân biệt chính xác các phân tử kiral trong một khoang. Được hỗ trợ bởi các xung laser bổ sung, các trường khoang có thể phát triển thành các trạng thái kết hợp khác nhau với các dịch chuyển ngược dấu theo tính thuận tay của các phân tử. Do đó, tính thuận tay của các phân tử có thể được đọc ra bằng phép đo đồng pha trên khoang và xác suất thành công gần như bằng một mà không cần các trường khoang rất mạnh. Kết quả số cho thấy lược đồ này không nhạy cảm với lỗi, nhiễu và mất kết nối. Do đó, lược đồ này có thể cung cấp các góc nhìn hữu ích để phân biệt chính xác các phân tử kiral."} +{"idx": 1870970, "en": "Fourier ptychography microscopy is a powerful tool for wide-field and high-spatial-resolution imaging. It can achieve a large field of view and high-spatial-resolution imaging with a low numerical aperture objective by capturing a series of low-resolution (LR) images that contain the information of different spatial frequencies and then stitching them together in the Fourier domain. Furthermore, the phase information of the object can also be recovered simultaneously. In this Letter, we propose a method to realize adaptive high-dynamic-range (HDR) LR image acquisition with a red, green, and blue camera, which needs only single exposure for each light-emitting diode (LED) illumination. According to the imaging principle of a color camera, the filter of each color channel still allows additional light within a certain wavelength range to pass through, but with a much smaller transmittance. By illuminating the sample with monochromatic light and combining the raw data from three color channels together, an HDR image corresponding to each LED illumination is obtained. The feasibility and good performance of our method are demonstrated by the experimental results.", "gemini": "Kính hiển vi chụp ảnh cắt lớp Fourier là một công cụ mạnh mẽ để chụp ảnh trường rộng và độ phân giải không gian cao. Nó có thể đạt được trường nhìn lớn và hình ảnh độ phân giải không gian cao với vật kính có khẩu độ số thấp bằng cách chụp một loạt các hình ảnh độ phân giải thấp (LR) chứa thông tin của các tần số không gian khác nhau và sau đó ghép chúng lại với nhau trong miền Fourier. Hơn nữa, thông tin pha của vật thể cũng có thể được phục hồi đồng thời. Trong Thư này, chúng tôi đề xuất một phương pháp để thực hiện thu nhận hình ảnh LR dải động cao (HDR) thích ứng với máy ảnh màu đỏ, xanh lá cây và xanh lam, chỉ cần một lần phơi sáng cho mỗi đèn phát sáng (LED). Theo nguyên tắc chụp ảnh của máy ảnh màu, bộ lọc của từng kênh màu vẫn cho phép thêm ánh sáng trong một phạm vi bước sóng nhất định đi qua, nhưng với độ truyền qua nhỏ hơn nhiều. Bằng cách chiếu sáng mẫu bằng ánh sáng đơn sắc và kết hợp dữ liệu thô từ ba kênh màu lại với nhau, thu được một hình ảnh HDR tương ứng với mỗi đèn LED chiếu sáng. Tính khả thi và hiệu suất tốt của phương pháp của chúng tôi đã được chứng minh bằng các kết quả thử nghiệm."} +{"idx": 1870971, "en": "In this Letter, a novel five-dimensional (5D) data-iteration-based encryption model is proposed at physical layer for multi-wavelength optical frequency division multiplexing passive optical network (OFDM-PON) by using a hyperchaotic system. The proposed scheme can generate five chaotic sequences at a time. The sensitivity of 10-18 can be achieved, along with a key space of 1095. In addition, we use a multi-wavelength channel to transmit the information, and the optical network unit can freely choose the wavelength. The probability shaping technology has greatly improved the bit error rate performance. A 16Gb/s/λ data is successfully transmitted across 25 km standard single-mode fiber in the experimental verifications. Therefore, it will have a positive impact in the future security optical network.", "gemini": "Trong Thư này, một mô hình mã hóa dựa trên lặp lại dữ liệu năm chiều (5D) mới được đề xuất ở lớp vật lý cho mạng quang thụ động phân chia tần số quang đa bước sóng (OFDM-PON) bằng cách sử dụng hệ thống siêu hỗn loạn. Sơ đồ được đề xuất có thể tạo ra năm chuỗi hỗn loạn cùng một lúc. Độ nhạy của 10-18 có thể đạt được, cùng với không gian khóa là 1095. Ngoài ra, chúng tôi sử dụng một kênh đa bước sóng để truyền thông tin và đơn vị mạng quang có thể tự do lựa chọn bước sóng. Công nghệ định hình xác suất đã cải thiện đáng kể hiệu suất tốc độ lỗi bit. Dữ liệu 16Gb/s/λ được truyền thành công qua 25 km sợi quang đơn mode chuẩn trong các xác minh thực nghiệm. Do đó, nó sẽ có tác động tích cực đến mạng quang an toàn trong tương lai."} +{"idx": 1870972, "en": "[This corrects the article DOI: 10.1371/journal.pntd.0005657.].", "gemini": "[Bài viết này sửa lỗi DOI: 10.1371/journal.pntd.0005657.]."} +{"idx": 1870973, "en": "[This corrects the article DOI: 10.1371/journal.pntd.0005468.].", "gemini": "[Bài viết này sửa lỗi DOI: 10.1371/journal.pntd.0005468.]."} +{"idx": 1870974, "en": "[This corrects the article DOI: 10.1371/journal.pntd.0007714.].", "gemini": "[Bài viết này sửa lỗi DOI: 10.1371/journal.pntd.0007714.]."} +{"idx": 1870975, "en": "The multifactorial aspects of breastfeeding require measures at many levels to identify mothers in need of breastfeeding support from healthcare professionals. Our objective was to examine the relative importance of sociodemographic, pre/perinatal-, infant-, psychosocial-, and interaction-related factors affecting duration of exclusive breastfeeding. We used self-reported data from a community-based trial including 1265 women (response rate 49%) giving birth from January 2017 to February 2018. Data on outcome, duration of exclusive breastfeeding, were collected three and nine months postpartum; data on the study variables concerning known risk factors for breastfeeding cessation were collected two weeks postpartum. Crude and multiple Cox proportional hazards models were used for statistical analyses with additional analyses for time varying effects. Factors with an independent prognostic influence on duration of exclusive breastfeeding in fully adjusted models included early skin-to-skin contact (HR = 1.18 CI:1.04-1.33), intention to breastfeed (HR = 0.77 CI: 0.73-0.80), positive outcome evaluation, meaning the value mothers attributed to breastfeeding (HR = 1.33 CI: 1.08-1.63), higher level of self-efficacy (HR = 1.46 CI: 1.24-1.72), and maternal sense of security in relation to breastfeeding (HR = 1.31 CI: 1.14-1.50). Higher maternal BMI, lower self-efficacy, shorter breastfeeding duration of previous child, and hospitalization during birth were time dependent by affecting the exclusive breastfeeding duration primarily in the first months following birth. The results suggest that target groups in special need of early breastfeeding support are defined by being hospitalized, obese, having low self-efficacy or short previous breastfeeding experience. The extensive influence of psychosocial factors emphasizes the importance of including both practical facilitating guidance and positive verbal encouragement to ensure effective breastfeeding support.", "gemini": "Các khía cạnh đa yếu tố của việc cho con bú đòi hỏi các biện pháp ở nhiều cấp độ để xác định những bà mẹ cần được các chuyên gia chăm sóc sức khỏe hỗ trợ cho con bú. Mục tiêu của chúng tôi là xem xét tầm quan trọng tương đối của các yếu tố nhân khẩu học, tiền sản/chuẩn bị sinh, trẻ sơ sinh, tâm lý xã hội và tương tác liên quan đến thời gian cho con bú hoàn toàn. Chúng tôi đã sử dụng dữ liệu tự báo cáo từ một thử nghiệm dựa trên cộng đồng bao gồm 1265 phụ nữ (tỷ lệ phản hồi 49%) sinh con từ tháng 1 năm 2017 đến tháng 2 năm 2018. Dữ liệu về kết quả, thời gian cho con bú hoàn toàn, được thu thập vào ba và chín tháng sau sinh; dữ liệu về các biến nghiên cứu liên quan đến các yếu tố nguy cơ đã biết đối với việc ngừng cho con bú được thu thập hai tuần sau sinh. Các mô hình nguy cơ tỷ lệ Cox thô và đa đã được sử dụng để phân tích thống kê với các phân tích bổ sung cho các hiệu ứng thay đổi theo thời gian. Các yếu tố có ảnh hưởng tiên lượng độc lập đến thời gian cho con bú hoàn toàn trong các mô hình được điều chỉnh đầy đủ bao gồm tiếp xúc da kề da sớm (HR = 1,18 CI: 1,04-1,33), ý định cho con bú (HR = 0,77 CI: 0,73-0,80), đánh giá kết quả tích cực, nghĩa là giá trị mà các bà mẹ gán cho việc cho con bú (HR = 1,33 CI: 1,08-1,63), mức độ tự tin cao hơn (HR = 1,46 CI: 1,24-1,72) và cảm giác an toàn của người mẹ liên quan đến việc cho con bú (HR = 1,31 CI: 1,14-1,50). Chỉ số BMI của bà mẹ cao hơn, mức độ tự tin thấp hơn, thời gian cho con bú ngắn hơn của đứa con trước và nhập viện khi sinh là những yếu tố phụ thuộc thời gian bằng cách ảnh hưởng đến thời gian cho con bú hoàn toàn chủ yếu trong những tháng đầu sau sinh. Kết quả cho thấy các nhóm đối tượng có nhu cầu đặc biệt về hỗ trợ cho con bú sớm được xác định là nhập viện, béo phì, có mức độ tự tin thấp hoặc kinh nghiệm cho con bú trước đó ngắn. Ảnh hưởng sâu rộng của các yếu tố tâm lý xã hội nhấn mạnh tầm quan trọng của việc bao gồm cả hướng dẫn tạo điều kiện thực tế và sự động viên bằng lời tích cực để đảm bảo hỗ trợ cho con bú hiệu quả."} +{"idx": 1870976, "en": "The widespread legalization of Cannabis has opened the industry to using contemporary analytical techniques for chemotype analysis. Chemotypic data has been collected on a large variety of oil profiles inherent to the cultivars that are commercially available. The unknown gene regulation and pharmacokinetics of dozens of cannabinoids offer opportunities of high interest in pharmacology research. Retailers in many medical and recreational jurisdictions are typically required to report chemical concentrations of at least some cannabinoids. Commercial cannabis laboratories have collected large chemotype datasets of diverse Cannabis cultivars. In this work a data set of 17,600 cultivars tested by Steep Hill Inc., is examined using machine learning techniques to interpolate missing chemotype observations and cluster cultivars into groups based on chemotype similarity. The results indicate cultivars cluster based on their chemotypes, and that some imputation methods work better than others at grouping these cultivars based on chemotypic identity. Due to the missing data and to the low signal to noise ratio for some less common cannabinoids, their behavior could not be accurately predicted. These findings have implications for characterizing complex interactions in cannabinoid biosynthesis and improving phenotypical classification of Cannabis cultivars.", "gemini": "Việc hợp pháp hóa cần sa trên diện rộng đã mở ra ngành công nghiệp sử dụng các kỹ thuật phân tích đương đại để phân tích kiểu hình. Dữ liệu kiểu hình đã được thu thập trên nhiều loại cấu hình dầu có sẵn trên thị trường. Quy định gen và dược động học chưa biết của hàng chục cannabinoid mang lại những cơ hội rất được quan tâm trong nghiên cứu dược lý. Các nhà bán lẻ ở nhiều khu vực y tế và giải trí thường được yêu cầu báo cáo nồng độ hóa học của ít nhất một số cannabinoid. Các phòng thí nghiệm cần sa thương mại đã thu thập các bộ dữ liệu kiểu hình lớn của các giống cần sa đa dạng. Trong công trình này, một tập dữ liệu gồm 17.600 giống được Steep Hill Inc. kiểm tra, được kiểm tra bằng các kỹ thuật học máy để nội suy các quan sát kiểu hình bị thiếu và nhóm các giống thành các nhóm dựa trên sự giống nhau về kiểu hình. Kết quả chỉ ra các giống nhóm dựa trên kiểu hình của chúng và một số phương pháp quy imputation hoạt động tốt hơn các phương pháp khác trong việc nhóm các giống này dựa trên bản sắc kiểu hình. Do dữ liệu bị thiếu và tỷ lệ tín hiệu trên nhiễu thấp đối với một số cannabinoid ít phổ biến hơn, nên không thể dự đoán chính xác hành vi của chúng. Những phát hiện này có ý nghĩa đối với việc mô tả các tương tác phức tạp trong quá trình tổng hợp cannabinoid và cải thiện phân loại kiểu hình của các giống cần sa."} +{"idx": 1870977, "en": "The information available through our senses is noisy, incomplete, and to varying degrees ambiguous. The perceptual system must create stable and reliable percepts out of this restricted information. It solves this perceptual inference problem by integrating memories of previous percepts and making predictions about the perceptual future. Using ambiguous figures and a new experimental approach, we studied whether generating predictions based on regularities in the past affects processing of the present and how this is done. Event-related potentials (ERPs) were measured to investigate whether a highly regular temporal context of either ambiguous or unambiguous stimulus variants differently affects processing of a current stimulus and/or task execution. Further, we tested whether symbolic announcements about the immediate perceptual future can replace the past experience of regularities as a source for making predictions. Both ERP and reaction time varied as a function of stimulus ambiguity in the temporal context of a present stimulus. No such effects were found with symbolic announcements. Our results indicate that predictions about the future automatically alter processing of the present, even if the predictions are irrelevant for the present percept and task. However, direct experiences of past regularities are necessary for predicting the future whereas symbolic information about the future is not sufficient.", "gemini": "Thông tin có được thông qua các giác quan của chúng ta thường nhiễu, không đầy đủ và ở các mức độ khác nhau là mơ hồ. Hệ thống nhận thức phải tạo ra các nhận thức ổn định và đáng tin cậy từ thông tin hạn chế này. Nó giải quyết vấn đề suy luận nhận thức này bằng cách tích hợp các ký ức về các nhận thức trước đó và đưa ra dự đoán về tương lai nhận thức. Sử dụng các hình ảnh mơ hồ và một phương pháp tiếp cận thử nghiệm mới, chúng tôi đã nghiên cứu liệu việc đưa ra dự đoán dựa trên các quy luật trong quá khứ có ảnh hưởng đến quá trình xử lý hiện tại hay không và cách thực hiện điều này. Các điện thế sự kiện liên quan (ERP) đã được đo để điều tra xem bối cảnh thời gian rất đều đặn của các biến thể kích thích mơ hồ hoặc không mơ hồ có ảnh hưởng khác nhau đến quá trình xử lý kích thích hiện tại và/hoặc thực hiện nhiệm vụ hay không. Hơn nữa, chúng tôi đã kiểm tra xem các thông báo tượng trưng về tương lai nhận thức trực tiếp có thể thay thế kinh nghiệm trong quá khứ về các quy luật như một nguồn để đưa ra dự đoán hay không. Cả ERP và thời gian phản ứng đều thay đổi theo chức năng của sự mơ hồ của kích thích trong bối cảnh thời gian của một kích thích hiện tại. Không có hiệu ứng nào như vậy được tìm thấy với các thông báo tượng trưng. Kết quả của chúng tôi chỉ ra rằng các dự đoán về tương lai tự động thay đổi quá trình xử lý hiện tại, ngay cả khi các dự đoán không liên quan đến nhận thức và nhiệm vụ hiện tại. Tuy nhiên, những kinh nghiệm trực tiếp về các quy luật trong quá khứ là cần thiết để dự đoán tương lai trong khi thông tin tượng trưng về tương lai là không đủ."} +{"idx": 1870978, "en": "The prevalence of metabolic syndrome (MetS) is increasing worldwide, and diet therapy plays a key role in treating this disease. Since most patients show difficulties in adhering to nutritional interventions, research on the association of positive psychological characteristics with greater engagement in physical health is relevant to this field. The present study aimed to evaluate the association between positive psychology attributes (optimism, hope, self-esteem, positive/negative affect and life satisfaction) and changes in diet quality and anthropometric parameters of individuals with MetS who received nutritional counseling. The study assessed 63 patients at a nutrition outpatient clinic. Anthropometric parameters and 24-hour food recall data (for evaluation of the Brazilian Healthy Eating Index-Revised-BHEI-R) were collected at the first visit and subsequent return visit (on average five months later). Psychological data were collected at the first visit using validated and standardized scales. The results were adjusted in relation to the depression scores of the patients, which were evaluated using the Beck Depression Inventory-II (BDI-II). Changes in anthropometric factors and in the BHEI-R were assessed, and their associations with the psychological attributes were investigated. The results indicated that positive affect and hope were associated with improvement in the BHEI-R scores (Cohen effect sizes -0.65 and -0.58; p = 0.012 and 0.025, respectively). A significant association was also observed between optimism and a reduction in abdominal circumference (Cohen effect size 0.56; p = 0.031). The associations remained significant even after adjusting for the BDI-II scores (p = 0.022, p = 0.037 and p = 0.05, respectively). No statistically significant associations were observed for the other attributes assessed.The study suggests that some attributes may have a greater influence on the nutritional treatment of MetS and that future studies should be conducted in order to enable effective multidisciplinary interventions to treat MetS.", "gemini": "Tỷ lệ mắc hội chứng chuyển hóa (MetS) đang gia tăng trên toàn thế giới và chế độ ăn uống đóng vai trò chính trong việc điều trị căn bệnh này. Vì hầu hết bệnh nhân đều gặp khó khăn trong việc tuân thủ các biện pháp can thiệp dinh dưỡng, nên việc nghiên cứu mối liên quan giữa các đặc điểm tâm lý tích cực với sự tham gia nhiều hơn vào sức khỏe thể chất có liên quan đến lĩnh vực này. Nghiên cứu hiện tại nhằm đánh giá mối liên quan giữa các thuộc tính tâm lý tích cực (lòng lạc quan, hy vọng, lòng tự trọng, tình cảm tích cực/tiêu cực và sự hài lòng trong cuộc sống) và những thay đổi về chất lượng chế độ ăn uống và các thông số nhân trắc học của những người mắc MetS đã được tư vấn dinh dưỡng. Nghiên cứu đã đánh giá 63 bệnh nhân tại một phòng khám dinh dưỡng dành cho bệnh nhân ngoại trú. Các thông số nhân trắc học và dữ liệu thu hồi thực phẩm trong 24 giờ (để đánh giá Chỉ số ăn uống lành mạnh của Brazil-Phiên bản sửa đổi-BHEI-R) đã được thu thập tại lần khám đầu tiên và lần tái khám tiếp theo (trung bình là năm tháng sau). Dữ liệu tâm lý được thu thập tại lần khám đầu tiên bằng các thang đo đã được chuẩn hóa và xác thực. Kết quả đã được điều chỉnh theo điểm trầm cảm của bệnh nhân, được đánh giá bằng Bản kiểm kê trầm cảm Beck-II (BDI-II). Những thay đổi về các yếu tố nhân trắc học và BHEI-R đã được đánh giá và mối liên quan của chúng với các thuộc tính tâm lý đã được nghiên cứu. Kết quả cho thấy tình cảm tích cực và hy vọng có liên quan đến sự cải thiện điểm số BHEI-R (kích thước hiệu ứng Cohen -0,65 và -0,58; tương ứng là p = 0,012 và 0,025). Một mối liên quan đáng kể cũng được quan sát thấy giữa sự lạc quan và việc giảm chu vi vòng bụng (kích thước hiệu ứng Cohen 0,56; p = 0,031). Các mối liên quan vẫn có ý nghĩa ngay cả sau khi điều chỉnh điểm số BDI-II (tương ứng là p = 0,022, p = 0,037 và p = 0,05). Không có mối liên quan nào có ý nghĩa thống kê nào được quan sát thấy đối với các thuộc tính khác được đánh giá. Nghiên cứu gợi ý rằng một số thuộc tính có thể có ảnh hưởng lớn hơn đến việc điều trị dinh dưỡng của MetS và các nghiên cứu trong tương lai nên được tiến hành để cho phép các biện pháp can thiệp đa ngành hiệu quả để điều trị MetS."} +{"idx": 1870979, "en": "[This corrects the article DOI: 10.1371/journal.pntd.0007025.].", "gemini": "[Điều này sửa đổi bài báo có DOI: 10.1371/journal.pntd.0007025.]."} +{"idx": 1870980, "en": "BACKGROUND There is a need for a rapid diagnostic point of care test to detect Neisseria gonorrhoeae (NG) infection to prevent incorrect, lack or excess of treatment resulting from current syndromic management in low-resource settings. An assay to identify NG antimicrobial resistance (AMR) is also highly desirable to facilitate antibiotic stewardship. Here we describe the development of two target product profiles (TPPs): one for a test for etiological diagnosis of NG and Chlamydia trachomatis (CT) (TPP1) and one for the detection of NG AMR/susceptibility (TPP2). METHODS Draft TPPs were initially developed based on a landscape analysis of existing diagnostics and expert input. TPPs were refined via an online Delphi survey with two rounds of input from 68 respondents. TPP characteristics on which <75% of non-industry respondents agreed were further discussed and revised by an expert working group. RESULTS The need for a test to identify NG in patients with urethral or vaginal discharge was identified as a minimal requirement of TPP1, with a test that can diagnose NG in asymptomatic patients as the optimal requirement. A sensitivity of 80% was considered acceptable, either in context of syndromic management or screening high-risk populations. For TPP2, the agreed minimal requirement was for a test to be used at level 2 healthcare facilities and above, with an optimal requirement of level 1 or above. A lateral flow format was preferred for TPP1, while it was considered likely that TPP2 would require a molecular format. A total of 31 test characteristics were included in TPP1 and 27 in TPP2. CONCLUSIONS Following the working group revisions, TPPs were posted online for public feedback for two months, and are now finalized. The final TPPs are currently guiding the development of new diagnostics that meet the defined characteristics to reach the market within two years.", "gemini": "BỐI CẢNH Cần có một xét nghiệm chẩn đoán nhanh tại điểm chăm sóc để phát hiện nhiễm Neisseria gonorrhoeae (NG) nhằm ngăn ngừa điều trị không chính xác, thiếu hoặc quá mức do cách quản lý hội chứng hiện tại ở những nơi có ít nguồn lực. Một xét nghiệm để xác định tình trạng kháng thuốc của NG (AMR) cũng rất được mong muốn để tạo điều kiện quản lý kháng sinh. Ở đây chúng tôi mô tả sự phát triển của hai hồ sơ sản phẩm mục tiêu (TPP): một hồ sơ dành cho xét nghiệm chẩn đoán nguyên nhân của NG và Chlamydia trachomatis (CT) (TPP1) và một hồ sơ dành cho việc phát hiện AMR/mẫn cảm của NG (TPP2). PHƯƠNG PHÁP Các TPP bản thảo ban đầu được phát triển dựa trên phân tích bối cảnh về các chẩn đoán hiện có và ý kiến chuyên gia. Các TPP đã được tinh chỉnh thông qua một cuộc khảo sát Delphi trực tuyến với hai vòng ý kiến từ 68 người trả lời. Các đặc điểm của TPP mà <75% số người trả lời không phải là người trong ngành đồng ý đã được một nhóm chuyên gia thảo luận và sửa đổi thêm. KẾT QUẢ Nhu cầu về một xét nghiệm để xác định NG ở những bệnh nhân bị dịch niệu đạo hoặc âm đạo được xác định là yêu cầu tối thiểu của TPP1, với một xét nghiệm có thể chẩn đoán NG ở những bệnh nhân không có triệu chứng là yêu cầu tối ưu. Độ nhạy 80% được coi là chấp nhận được, trong bối cảnh quản lý hội chứng hoặc sàng lọc những người có nguy cơ cao. Đối với TPP2, yêu cầu tối thiểu đã thống nhất là xét nghiệm được sử dụng tại các cơ sở y tế cấp 2 trở lên, với yêu cầu tối ưu là cấp 1 trở lên. Định dạng dòng chảy bên được ưu tiên cho TPP1, trong khi TPP2 được coi là có khả năng yêu cầu định dạng phân tử. Tổng cộng có 31 đặc điểm xét nghiệm được đưa vào TPP1 và 27 đặc điểm được đưa vào TPP2. KẾT LUẬN Sau khi nhóm làm việc sửa đổi, các TPP đã được đăng trực tuyến để công chúng phản hồi trong hai tháng và hiện đã được hoàn thiện. Các TPP cuối cùng hiện đang hướng dẫn việc phát triển các phương pháp chẩn đoán mới đáp ứng các đặc điểm đã xác định để đưa ra thị trường trong vòng hai năm."} +{"idx": 1870981, "en": "The paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein-1 (MALT1) regulates nuclear-factor-kappa-B (NF-κB) activation downstream of surface receptors with immunoreceptor tyrosine-based activation motifs (ITAMs), such as the B-cell or T-cell receptor and has thus emerged as a therapeutic target for autoimmune diseases. However, recent reports demonstrate the development of lethal autoimmune inflammation due to the excessive production of interferon gamma (IFN-ɣ) and defective differentiation of regulatory T-cells in genetically modified mice deficient in MALT1 paracaspase activity. To address this issue, we explored the effects of pharmacological MALT1 inhibition on the balance between T-effector and regulatory T-cells. Here we demonstrate that allosteric inhibition of MALT1 suppressed Th1, Th17 and Th1/Th17 effector responses, and inhibited T-cell dependent B-cell proliferation and antibody production. Allosteric MALT1 inhibition did not interfere with the suppressive function of human T-regulatory cells, although it impaired de novo differentiation of regulatory T-cells from naïve T-cells. Treatment with an allosteric MALT1 inhibitor alleviated the cytokine storm, including IFN-ɣ, in a mouse model of acute T-cell activation, and long-term treatment did not lead to an increase in IFN-ɣ producing CD4 cells or tissue inflammation. Together, our data demonstrate that the effects of allosteric inhibition of MALT1 differ from those seen in mice with proteolytically inactive MALT1, and thus we believe that MALT1 is a viable target for B and T-cell driven autoimmune diseases.", "gemini": "Protein chuyển vị tế bào lympho liên quan đến niêm mạc paracaspase-1 (MALT1) điều chỉnh hoạt hóa nhân tố hạt nhân-kappa-B (NF-κB) theo hạ nguồn của thụ thể bề mặt với các họa tiết hoạt hóa dựa trên tyrosine của thụ thể miễn dịch (ITAM), chẳng hạn như tế bào B hoặc thụ thể tế bào T và do đó đã nổi lên như một mục tiêu điều trị cho các bệnh tự miễn. Tuy nhiên, các báo cáo gần đây chứng minh sự phát triển của tình trạng viêm tự miễn gây tử vong do sản xuất quá mức interferon gamma (IFN-ɣ) và sự biệt hóa khiếm khuyết của tế bào T điều hòa ở những con chuột được biến đổi gen thiếu hoạt động paracaspase MALT1. Để giải quyết vấn đề này, chúng tôi đã khám phá các tác động của ức chế MALT1 dược lý lên sự cân bằng giữa tế bào T hiệu ứng và tế bào T điều hòa. Ở đây chúng tôi chứng minh rằng ức chế dị lập thể của MALT1 ức chế đáp ứng hiệu ứng Th1, Th17 và Th1/Th17 và ức chế sự tăng sinh tế bào B phụ thuộc tế bào T và sản xuất kháng thể. Ức chế MALT1 dị lập thể không ảnh hưởng đến chức năng ức chế của tế bào T điều hòa ở người, mặc dù nó làm suy yếu sự biệt hóa tế bào T điều hòa mới từ tế bào T ngây thơ. Điều trị bằng chất ức chế MALT1 dị lập thể làm giảm cơn bão cytokine, bao gồm cả IFN-ɣ, ở một mô hình chuột bị hoạt hóa tế bào T cấp tính và điều trị dài hạn không dẫn đến sự gia tăng các tế bào CD4 sản xuất IFN-ɣ hoặc tình trạng viêm mô. Kết hợp lại, dữ liệu của chúng tôi chứng minh rằng các tác động của ức chế dị lập thể của MALT1 khác với những tác động được thấy ở những con chuột có MALT1 không hoạt động do thủy phân và do đó chúng tôi tin rằng MALT1 là một mục tiêu khả thi cho các bệnh tự miễn do tế bào B và tế bào T gây ra."} +{"idx": 1870982, "en": "INTRODUCTION Both recessive and dominant genetic forms of Parkinson's disease have been described. The aim of this study was to assess the contribution of several genes to the pathophysiology of early onset Parkinson's disease in a cohort from central Spain. METHODS/PATIENTS We analyzed a cohort of 117 unrelated patients with early onset Parkinson's disease using a pipeline, based on a combination of a next-generation sequencing panel of 17 genes previously related with Parkinson's disease and other Parkinsonisms and CNV screening. RESULTS Twenty-six patients (22.22%) carried likely pathogenic variants in PARK2, LRRK2, PINK1, or GBA. The gene most frequently mutated was PARK2, and p.Asn52Metfs*29 was the most common variation in this gene. Pathogenic variants were not observed in genes SNCA, FBXO7, PARK7, HTRA2, DNAJC6, PLA2G6, and UCHL1. Co-occurrence of pathogenic variants involving two genes was observed in ATP13A2 and PARK2 genes, as well as LRRK2 and GIGYF2 genes. CONCLUSIONS Our results contribute to the understanding of the genetic architecture associated with early onset Parkinson's disease, showing both PARK2 and LRRK2 play an important role in Spanish Parkinson's disease patients. Rare variants in ATP13A2 and GIGYF2 may contribute to PD risk. However, a large proportion of genetic components remains unknown. This study might contribute to genetic diagnosis and counseling for families with early onset Parkinson's disease.", "gemini": "GIỚI THIỆU Cả các dạng di truyền lặn và trội của bệnh Parkinson đều đã được mô tả. Mục đích của nghiên cứu này là đánh giá sự đóng góp của một số gen vào bệnh sinh của bệnh Parkinson khởi phát sớm trong một nhóm bệnh nhân từ miền trung Tây Ban Nha. PHƯƠNG PHÁP/BỆNH NHÂN Chúng tôi đã phân tích một nhóm 117 bệnh nhân không có quan hệ họ hàng mắc bệnh Parkinson khởi phát sớm bằng một quy trình, dựa trên sự kết hợp của một nhóm giải trình tự thế hệ tiếp theo gồm 17 gen trước đây có liên quan đến bệnh Parkinson và các bệnh Parkinson khác và sàng lọc CNV. KẾT QUẢ Hai mươi sáu bệnh nhân (22,22%) mang các biến thể gây bệnh có khả năng ở PARK2, LRRK2, PINK1 hoặc GBA. Gen đột biến thường xuyên nhất là PARK2 và p.Asn52Metfs*29 là biến thể phổ biến nhất ở gen này. Các biến thể gây bệnh không được quan sát thấy ở các gen SNCA, FBXO7, PARK7, HTRA2, DNAJC6, PLA2G6 và UCHL1. Sự đồng xuất hiện của các biến thể gây bệnh liên quan đến hai gen được quan sát thấy ở các gen ATP13A2 và PARK2, cũng như các gen LRRK2 và GIGYF2. KẾT LUẬN Kết quả của chúng tôi góp phần vào việc hiểu cấu trúc di truyền liên quan đến bệnh Parkinson khởi phát sớm, cho thấy cả PARK2 và LRRK2 đều đóng vai trò quan trọng ở những bệnh nhân mắc bệnh Parkinson người Tây Ban Nha. Các biến thể hiếm ở ATP13A2 và GIGYF2 có thể góp phần vào nguy cơ mắc bệnh PD. Tuy nhiên, một tỷ lệ lớn các thành phần di truyền vẫn chưa được biết. Nghiên cứu này có thể góp phần chẩn đoán di truyền và tư vấn cho các gia đình có bệnh Parkinson khởi phát sớm."} +{"idx": 1870983, "en": "Tonsillectomy is a commonly performed surgical procedure worldwide; however, the possible long-term consequences have not been fully explored. The tonsils are secondary lymphoid tissue providing a defensive barrier against pathogens. There are few data in the literature on the relationship between prior tonsillectomy and the risk of irritable bowel syndrome (IBS). The aim of this study was to evaluate the risk of developing IBS in patients who underwent tonsillectomy using a nationwide longitudinal population-based cohort. We identified 1300 patients with tonsillectomy and 2600 matched controls from the Longitudinal Health Insurance Research Database of the National Health Insurance Research Database in Taiwan. Propensity score analysis was used for matching age, gender, comorbidities, medications, and index year at a ratio of 1:2. Multiple Cox regression analysis was used to estimate the adjusted hazard ratio of IBS. Furthermore, sensitivity tests and a stratified analysis were conducted. The incidence of IBS was 3 per 1,000 person-years in tonsillectomy patients, which was higher than the rate of 1.8 per 1,000 person-years found in non-tonsillectomy patients. After adjustment for patients' age, gender, comorbidities, and medications, patients who underwent tonsillectomy had a 1.84-fold risk of developing IBS compared to those without tonsillectomy (adjusted hazard ratio [HR]:1.84; 95% CI, 1.09-3.09). Stratified analysis revealed that a higher adjusted HR of 3.79 (95% CI, 1.35-10.64) in patients ≥50 years old, and there was a marginally significantly higher adjusted HR of 1.98 (95% CI, 0.99-3.95) of developing IBS when the follow-up period was longer than 3 years. Tonsillectomy was associated with a higher risk of developing IBS. Physicians should provide appropriate monitoring of IBS in patients undergoing tonsillectomy.", "gemini": "Phẫu thuật cắt amidan là một thủ thuật phẫu thuật thường được thực hiện trên toàn thế giới; tuy nhiên, các hậu quả có thể xảy ra trong thời gian dài vẫn chưa được khám phá đầy đủ. Amidan là mô bạch huyết thứ cấp tạo ra hàng rào phòng thủ chống lại các tác nhân gây bệnh. Có rất ít dữ liệu trong tài liệu về mối quan hệ giữa cắt amidan trước đó và nguy cơ mắc hội chứng ruột kích thích (IBS). Mục đích của nghiên cứu này là đánh giá nguy cơ phát triển IBS ở những bệnh nhân đã cắt amidan bằng cách sử dụng nhóm đối chứng theo dõi theo chiều dọc trên toàn quốc. Chúng tôi đã xác định 1300 bệnh nhân cắt amidan và 2600 đối chứng được ghép nối từ Cơ sở dữ liệu nghiên cứu bảo hiểm y tế theo chiều dọc của Cơ sở dữ liệu nghiên cứu bảo hiểm y tế quốc gia tại Đài Loan. Phân tích điểm khuynh hướng được sử dụng để ghép tuổi, giới tính, bệnh đi kèm, thuốc và năm chỉ số theo tỷ lệ 1:2. Phân tích hồi quy Cox đa biến được sử dụng để ước tính tỷ lệ nguy cơ điều chỉnh của IBS. Hơn nữa, các xét nghiệm độ nhạy và phân tích phân tầng đã được tiến hành. Tỷ lệ mắc IBS là 3 trên 1.000 người-năm ở những bệnh nhân cắt amidan, cao hơn tỷ lệ 1,8 trên 1.000 người-năm được tìm thấy ở những bệnh nhân không cắt amidan. Sau khi điều chỉnh theo tuổi, giới tính, bệnh đi kèm và thuốc của bệnh nhân, những bệnh nhân đã cắt amidan có nguy cơ phát triển IBS cao gấp 1,84 lần so với những bệnh nhân không cắt amidan (tỷ lệ nguy cơ điều chỉnh [HR]: 1,84; 95% CI, 1,09-3,09). Phân tích phân tầng cho thấy HR điều chỉnh cao hơn là 3,79 (95% CI, 1,35-10,64) ở những bệnh nhân ≥50 tuổi và có HR điều chỉnh cao hơn đáng kể là 1,98 (95% CI, 0,99-3,95) khi phát triển IBS khi thời gian theo dõi dài hơn 3 năm. Cắt amidan có liên quan đến nguy cơ phát triển IBS cao hơn. Các bác sĩ nên theo dõi IBS thích hợp ở những bệnh nhân cắt amidan."} +{"idx": 1870984, "en": "Although endocranial abnormal blood vessel impressions (ABVIs) and periosteal appositions (PAs) have been considered as paleopathological diagnostic criteria for tuberculous meningitis (TBM) based on findings of previous studies, they are not pathognomonic for tuberculosis (TB). Therefore, their utilization in the paleopathological practice can be questioned, especially in consideration that most of the previous studies were not performed on identified skeletal collections but on osteoarchaeological material and did not include statistical data analysis. To fill the aforementioned research gap, for the first time, a macroscopic investigation was conducted on identified pre-antibiotic era skeletons from the Terry Collection. A sample set of 234 individuals who died of TB (TB group) and 193 individuals who died of non-tuberculous causes (NTB group) were examined. The frequency of ABVIs and PAs, as well as other probable TB-related lesions was recorded. To determine the significance of difference (if any) in the frequencies of ABVIs and PAs between the two groups, χ2 testing of our data was performed. We found that ABVIs, PAs, and their co-occurrence with each other and with other probable TB-related lesions were more common in the TB group than in the NTB group. In addition, the χ2 comparative frequencies of ABVIs and PAs revealed a statistically significant difference between individuals who died of TB and individuals who died of NTB causes. Our findings strengthen those of previous studies that ABVIs and PAs are not specific to TBM but can be of tuberculous origin. Therefore, they do have a diagnostic value in the identification of TB in human osteoarchaeological material, especially when they simultaneously occur with other probable TB-related lesions. Their prudent utilization provides paleopathologists with a stronger basis for diagnosing TB and consequently, a more sensitive means of assessing TB frequency in past human populations.", "gemini": "Mặc dù dấu ấn mạch máu bất thường trong sọ (ABVI) và sự tích tụ màng xương (PA) được coi là tiêu chuẩn chẩn đoán bệnh lý cổ xưa cho bệnh viêm màng não do lao (TBM) dựa trên các phát hiện của các nghiên cứu trước đây, nhưng chúng không phải là dấu hiệu đặc hiệu cho bệnh lao (TB). Do đó, việc sử dụng chúng trong thực hành bệnh lý cổ xưa có thể bị nghi ngờ, đặc biệt khi xem xét rằng hầu hết các nghiên cứu trước đây không được thực hiện trên các bộ sưu tập xương đã xác định mà trên vật liệu khảo cổ học xương và không bao gồm phân tích dữ liệu thống kê. Để lấp đầy khoảng trống nghiên cứu đã đề cập, lần đầu tiên, một cuộc điều tra vĩ mô đã được tiến hành trên các bộ xương thời kỳ tiền kháng sinh đã xác định từ Bộ sưu tập Terry. Một tập hợp mẫu gồm 234 cá nhân tử vong vì TB (nhóm TB) và 193 cá nhân tử vong vì các nguyên nhân không do lao (nhóm NTB) đã được kiểm tra. Tần suất của ABVI và PA, cũng như các tổn thương khác có thể liên quan đến TB đã được ghi lại. Để xác định ý nghĩa của sự khác biệt (nếu có) về tần suất của ABVI và PA giữa hai nhóm, chúng tôi đã thực hiện kiểm định χ2 đối với dữ liệu của mình. Chúng tôi thấy rằng ABVI, PA và sự đồng xuất hiện của chúng với nhau và với các tổn thương khác có thể liên quan đến TB phổ biến hơn ở nhóm TB so với nhóm NTB. Ngoài ra, tần suất so sánh χ2 của ABVI và PA cho thấy sự khác biệt có ý nghĩa thống kê giữa những người tử vong vì TB và những người tử vong vì các nguyên nhân NTB. Những phát hiện của chúng tôi củng cố những phát hiện của các nghiên cứu trước đây rằng ABVI và PA không đặc hiệu đối với TBM nhưng có thể có nguồn gốc từ lao. Do đó, chúng có giá trị chẩn đoán trong việc xác định TB trong vật liệu khảo cổ học xương của con người, đặc biệt là khi chúng đồng thời xuất hiện với các tổn thương khác có thể liên quan đến TB. Việc sử dụng thận trọng của chúng cung cấp cho các nhà bệnh lý cổ xưa cơ sở vững chắc hơn để chẩn đoán TB và do đó, có phương tiện nhạy hơn để đánh giá tần suất TB trong quần thể người trong quá khứ."} +{"idx": 1870985, "en": "In this study, the effect of glazing-to-total-wall-area ratio on the thermal performance of different wall materials is numerically investigated in terms of heat transmission load. The investigation was performed for a South-facing wall in Elazığ, Turkey. The heat transmission load through walls and windows are determined separately for summer and winter climate conditions. In this analysis, the frame area of the window is not considered. Therefore, whereas the glazing area on uninsulated and insulated walls is increased from 0% to 100%, the heat gain and losses are calculated separately according to the glazing type. The transmission loads through the wall are determined by an implicit finite difference procedure under steady periodic conditions. Concrete, briquettes, bricks, and autoclaved aerated concrete are selected as structure materials. Results show that in the uninsulated wall, the wall material affected the glazing area, whereas in the insulated wall, the effect of wall material on glazing area is insignificant.", "gemini": "Trong nghiên cứu này, tác động của tỷ lệ diện tích kính trên tổng diện tích tường đối với hiệu suất nhiệt của các vật liệu tường khác nhau được nghiên cứu số về tải trọng truyền nhiệt. Cuộc điều tra được thực hiện đối với một bức tường hướng Nam ở Elazığ, Thổ Nhĩ Kỳ. Tải trọng truyền nhiệt qua tường và cửa sổ được xác định riêng cho điều kiện khí hậu mùa hè và mùa đông. Trong phân tích này, diện tích khung của cửa sổ không được xem xét. Do đó, trong khi diện tích kính trên các bức tường không cách nhiệt và cách nhiệt tăng từ 0% đến 100%, thì mức tăng và giảm nhiệt được tính riêng theo loại kính. Tải trọng truyền qua tường được xác định bằng quy trình hiệu chỉnh hữu hạn ngầm trong điều kiện chu kỳ ổn định. Bê tông, gạch, gạch xây và bê tông khí chưng áp được chọn làm vật liệu kết cấu. Kết quả cho thấy ở bức tường không cách nhiệt, vật liệu tường ảnh hưởng đến diện tích kính, trong khi ở bức tường cách nhiệt, ảnh hưởng của vật liệu tường đến diện tích kính là không đáng kể."} +{"idx": 1870986, "en": "[This corrects the article DOI: 10.1371/journal.pntd.0008129.].", "gemini": "[Điều này sửa bài viết DOI: 10.1371/journal.pntd.0008129.]."} +{"idx": 1870987, "en": "OBJECTIVES Pertussis remains endemic despite high vaccine coverage in infants and toddlers. Pertussis vaccines confer protection but immunity wanes overtime and boosters are needed in a lifetime. Iran, eligible for the Expanded Program on Immunization that includes the primary immunization, implemented two additional booster doses using a whole-cell vaccine (wPV) at 18 months-old and about 6 years-old. Duration of protection induced by the wPVs currently in use and their impact as pre-school booster are not well documented. This study aimed at assessing vaccination compliance and at estimating the duration of protection conferred by vaccination with wPV in children aged < 15 years in Tehran, Iran. METHODS Detailed information on vaccination history and capillary blood samples were obtained from 1047 children aged 3-15 years who completed the 3 doses-primary pertussis immunization, in Tehran. Anti-pertussis toxin IgG levels were quantified by ELISA. RESULTS Compliance was very high with 93.3% of children who received the three primary and 1st booster doses in a timely manner. Timeliness of the 2nd booster was lower (63.3%). Rate of seropositive samples continuously and significantly increased from 1-2 to 5-6 years after 1st booster attaining 30.4% of children exhibiting serological sign of recent contact with B. pertussis. Second booster dating back 1 or 2 years was associated with high antibody titers, which significantly decreased within 3 years from injection. Among children who received 2nd booster injection more than 2 years before serum analysis, seroprevalence of pertussis infection was 8.4% and seropositivity rate was higher from the 10 years-old group. CONCLUSION Seropositivity in children aged 6-7 years with no 2nd booster supports the need for a vaccination at that age. Adolescent booster may also be considered.", "gemini": "MỤC TIÊU Ho gà vẫn là bệnh lưu hành mặc dù tỷ lệ tiêm vắc-xin cao ở trẻ sơ sinh và trẻ mới biết đi. Vắc-xin ho gà mang lại khả năng bảo vệ nhưng khả năng miễn dịch giảm dần theo thời gian và cần tiêm nhắc lại suốt đời. Iran, đủ điều kiện tham gia Chương trình tiêm chủng mở rộng bao gồm tiêm chủng cơ bản, đã thực hiện thêm hai liều nhắc lại bằng vắc-xin toàn tế bào (wPV) khi trẻ được 18 tháng tuổi và khoảng 6 tuổi. Thời gian bảo vệ do wPV hiện đang sử dụng mang lại và tác động của chúng như một mũi tiêm nhắc lại trước khi đi học vẫn chưa được ghi chép đầy đủ. Nghiên cứu này nhằm mục đích đánh giá mức độ tuân thủ tiêm chủng và ước tính thời gian bảo vệ do tiêm chủng wPV ở trẻ em dưới 15 tuổi tại Tehran, Iran. PHƯƠNG PHÁP Thu thập thông tin chi tiết về tiền sử tiêm chủng và mẫu máu mao mạch từ 1047 trẻ em từ 3-15 tuổi đã hoàn thành tiêm chủng ho gà cơ bản 3 liều tại Tehran. Nồng độ IgG kháng độc tố ho gà được định lượng bằng ELISA. KẾT QUẢ Tỷ lệ tuân thủ rất cao với 93,3% trẻ em đã nhận được ba liều cơ bản và liều nhắc lại đầu tiên đúng thời hạn. Tỷ lệ đúng thời hạn của mũi nhắc lại thứ 2 thấp hơn (63,3%). Tỷ lệ mẫu huyết thanh dương tính liên tục và tăng đáng kể từ 1-2 đến 5-6 năm sau mũi nhắc lại thứ nhất đạt 30,4% trẻ em có dấu hiệu huyết thanh tiếp xúc gần đây với B. pertussis. Mũi nhắc lại thứ hai cách đây 1 hoặc 2 năm có liên quan đến nồng độ kháng thể cao, giảm đáng kể trong vòng 3 năm kể từ khi tiêm. Trong số những trẻ em được tiêm nhắc lại lần 2 hơn 2 năm trước khi xét nghiệm huyết thanh, tỷ lệ huyết thanh nhiễm trùng ho gà là 8,4% và tỷ lệ huyết thanh dương tính cao hơn ở nhóm trẻ 10 tuổi. KẾT LUẬN Tỷ lệ huyết thanh dương tính ở trẻ em từ 6-7 tuổi không có mũi nhắc lại thứ 2 chứng minh nhu cầu tiêm vắc-xin ở độ tuổi đó. Cũng có thể cân nhắc tiêm nhắc lại cho thanh thiếu niên."} +{"idx": 1870988, "en": "Symmetric dimethylarginine (SDMA) is a serum biomarker of renal damage in dogs. Moreover, SDMA concentration is an independent predictor of development of severe heart failure (HF) in humans with cardiac disease. This study evaluates whether the serum concentration of SDMA in dogs with myxomatous mitral valve disease (MMVD) is influenced by the severity of heart disease, pulmonary hypertension (PH) and treatment of HF. A total of 99 client-owned dogs were included in this retrospective case-control study; 78 dogs were affected by MMVD and classified according to the American College of Veterinary Internal Medicine (ACVIM) guidelines, and 21 were healthy controls. For each dog, history, physical examination, complete blood count, biochemical profile, thoracic radiography, 6-lead standard electrocardiogram and trans-thoracic echocardiography were available. Comparisons were performed between groups of dogs belonging to different ACVIM stages and between dogs with and without PH. The median SDMA concentration was neither significantly different among groups of dogs in different disease stages (overall P = 0.010), nor among dogs with MMVD, nor between those with [14.5 μg/dl (10.5-18.8)] and without PH [13 μg/dl (9-17.2)] (P = 0.295). The concentration of SDMA did not differ between dogs when considering the combined effect of the ACVIM group and cardiac treatment (overall P = 0.486). Furthermore, no correlation was found between SDMA concentration and radiographic and echocardiographic parameters associated with increased MMVD severity. In conclusion, this study failed to demonstrate the presence of renal impairment in dogs with MMVD, and the increase in renal parameters in some dogs in the more advanced stage of MMVD could be attributed to pre-renal azotemia.", "gemini": "Dimethylarginine đối xứng (SDMA) là một dấu ấn sinh học trong huyết thanh của tổn thương thận ở chó. Hơn nữa, nồng độ SDMA là một yếu tố dự báo độc lập về sự phát triển của suy tim nặng (HF) ở người mắc bệnh tim. Nghiên cứu này đánh giá xem nồng độ SDMA trong huyết thanh ở những con chó mắc bệnh van hai lá do thoái hóa dạng nhầy (MMVD) có bị ảnh hưởng bởi mức độ nghiêm trọng của bệnh tim, tăng huyết áp phổi (PH) và điều trị HF hay không. Tổng cộng 99 con chó thuộc sở hữu của khách hàng đã được đưa vào nghiên cứu đối chứng hồi cứu này; 78 con chó bị MMVD và được phân loại theo hướng dẫn của Học viện Thú y Nội khoa Hoa Kỳ (ACVIM), và 21 con là nhóm đối chứng khỏe mạnh. Đối với mỗi con chó, tiền sử, khám sức khỏe, công thức máu toàn bộ, hồ sơ sinh hóa, chụp X-quang ngực, điện tâm đồ chuẩn 6 đầu và siêu âm tim xuyên thành ngực đều có sẵn. Các so sánh được thực hiện giữa các nhóm chó thuộc các giai đoạn ACVIM khác nhau và giữa những con chó có và không có PH. Nồng độ SDMA trung bình không có sự khác biệt đáng kể giữa các nhóm chó ở các giai đoạn bệnh khác nhau (P tổng thể = 0,010), cũng không có sự khác biệt giữa những con chó bị MMVD, cũng như giữa những con có [14,5 μg/dl (10,5-18,8)] và không có PH [13 μg/dl (9-17,2)] (P = 0,295). Nồng độ SDMA không khác nhau giữa những con chó khi xem xét tác động kết hợp của nhóm ACVIM và điều trị tim (P tổng thể = 0,486). Hơn nữa, không tìm thấy mối tương quan nào giữa nồng độ SDMA và các thông số chụp X-quang và siêu âm tim liên quan đến mức độ nghiêm trọng của MMVD tăng lên. Tóm lại, nghiên cứu này không chứng minh được sự suy giảm chức năng thận ở những con chó mắc MMVD, và sự gia tăng các thông số về thận ở một số con chó ở giai đoạn tiến triển hơn của MMVD có thể là do thiểu niệu trước thận."} +{"idx": 1870989, "en": "In the dairy industry, feeding management has considerable influence on calf behavioral development, yet there is limited understanding of how aspects of diet or accommodating more varied feeding behavior may affect cognitive development in young calves. The objective of this study was to evaluate effects of provision and presentation of hay on the cognitive ability of pre-weaned dairy calves. Individually-housed Holstein heifer calves were assigned at birth to 1 of 3 treatments: pelleted starter only (n = 10), hay (chopped to 5 cm) and starter provided in separate buckets (n = 12), or hay and starter offered as a mixture (n = 11). During week 5 of age, calves were tested daily in a learning task consisting of a T-maze with a milk reward (0.2 L milk) placed in one arm. Calves were subjected to an initial learning and reversal learning stage, where the reward location was changed to the opposite arm of the maze. Calves received 5 sessions/d until they met learning criterion (moving directly to correct side in 3 consecutive sessions) for initial and reversal learning. Dietary treatment did not affect pass rate or the number of sessions required to pass the initial learning stage. During the reversal learning stage, calves provided only starter had a lower pass rate (0.038, during first 8 testing session) early during testing than calves provided hay separately (0.20; P = 0.020) and tended to have a lower pass rate than calves provided hay as a mixture (0.14; P = 0.057). Calves provided only starter also tended to require more sessions to meet the learning criterion (15.8) than both calves provided hay separately (10.8; P = 0.089) and as a mixture (11.8; P = 0.10). Calves provided hay also kicked less and spent more time sniffing or licking the testing area. The results of this experiment indicate that provision of hay may affect behavioral flexibility in dairy calves.", "gemini": "Trong ngành công nghiệp sữa, việc quản lý thức ăn có ảnh hưởng đáng kể đến sự phát triển hành vi của bê con, nhưng vẫn còn hạn chế hiểu biết về cách thức các khía cạnh của chế độ ăn hoặc việc thích ứng với hành vi ăn uống đa dạng hơn có thể ảnh hưởng đến sự phát triển nhận thức ở bê con. Mục tiêu của nghiên cứu này là đánh giá tác động của việc cung cấp và trình bày cỏ khô đối với khả năng nhận thức của bê sữa trước khi cai sữa. Những con bê cái giống bò sữa Holstein được nuôi riêng lẻ được chỉ định khi sinh cho 1 trong 3 chế độ điều trị: chỉ cho ăn thức ăn khởi đầu dạng viên (n = 10), cỏ khô (cắt thành từng đoạn 5 cm) và thức ăn khởi đầu được cung cấp trong các xô riêng biệt (n = 12), hoặc cỏ khô và thức ăn khởi đầu được cung cấp dưới dạng hỗn hợp (n = 11). Trong tuần thứ 5 tuổi, bê con được kiểm tra hàng ngày trong một bài kiểm tra học tập bao gồm mê cung chữ T với phần thưởng là sữa (0,2 lít sữa) được đặt ở một nhánh. Bê con được trải qua giai đoạn học tập ban đầu và giai đoạn học tập đảo ngược, trong đó vị trí phần thưởng được thay đổi sang nhánh đối diện của mê cung. Bê con được nhận 5 buổi/ngày cho đến khi đạt tiêu chuẩn học tập (di chuyển trực tiếp sang phía chính xác trong 3 buổi liên tiếp) cho giai đoạn học tập ban đầu và đảo ngược. Chế độ ăn không ảnh hưởng đến tỷ lệ vượt qua hoặc số buổi cần thiết để vượt qua giai đoạn học tập ban đầu. Trong giai đoạn học tập đảo ngược, những con bê chỉ được cho ăn thức ăn khởi đầu có tỷ lệ vượt qua thấp hơn (0,038, trong 8 buổi kiểm tra đầu tiên) vào đầu thời gian kiểm tra so với những con bê được cho ăn cỏ khô riêng biệt (0,20; P = 0,020) và có xu hướng có tỷ lệ vượt qua thấp hơn so với những con bê được cho ăn cỏ khô dưới dạng hỗn hợp (0,14; P = 0,057). Những con bê chỉ được cho ăn thức ăn khởi đầu cũng có xu hướng cần nhiều buổi hơn để đạt được tiêu chuẩn học tập (15,8) so với cả những con bê được cho ăn cỏ khô riêng biệt (10,8; P = 0,089) và dưới dạng hỗn hợp (11,8; P = 0,10). Những con bê được cho ăn cỏ khô cũng đá ít hơn và dành nhiều thời gian hơn để đánh hơi hoặc liếm khu vực thử nghiệm. Kết quả của thí nghiệm này chỉ ra rằng việc cung cấp cỏ khô có thể ảnh hưởng đến sự linh hoạt về hành vi ở bê sữa."} +{"idx": 1870990, "en": "The purpose of this study is to identify and characterize the structure and dynamics of global R&D collaboration networks in ICT by analyzing cross-country co-patents, with a special focus on the role of China. We employ a Social Network Analysis (SNA) perspective, using information on more than 77 thousand co-patents from 2001-2015. These co-patents are disaggregated by three time periods and four ICT subsectors. Global measures for the network as a whole, as well as local measures on the positioning of countries in the networks are interpreted. The empirical results are highly interesting. First, international R&D collaboration networks in ICT show a dynamic transformation in becoming larger in magnitude (more countries but also more inter-linkages), less centralized and more densely connected, though with varying degrees across ICT subsectors. Second, the powerful position of the US weakens relatively compared to other, increasingly connected countries, in particular China. While China has already surpassed the US in total patenting in ICT in 2015, China is now also catching up from a network perspective shown by its growing central position over the observed time period.", "gemini": "Mục đích của nghiên cứu này là xác định và mô tả cấu trúc và động lực của các mạng lưới hợp tác R&D toàn cầu trong CNTT bằng cách phân tích các bằng sáng chế chung giữa các quốc gia, đặc biệt tập trung vào vai trò của Trung Quốc. Chúng tôi sử dụng quan điểm Phân tích mạng xã hội (SNA), sử dụng thông tin về hơn 77 nghìn bằng sáng chế chung từ năm 2001-2015. Các bằng sáng chế chung này được phân tách theo ba giai đoạn thời gian và bốn phân ngành CNTT. Các biện pháp toàn cầu đối với toàn bộ mạng cũng như các biện pháp cục bộ về vị trí của các quốc gia trong mạng được diễn giải. Kết quả thực nghiệm rất thú vị. Đầu tiên, các mạng lưới hợp tác R&D quốc tế trong CNTT cho thấy một sự chuyển đổi năng động khi trở nên lớn hơn về quy mô (nhiều quốc gia hơn nhưng cũng có nhiều liên kết hơn), ít tập trung hơn và kết nối chặt chẽ hơn, mặc dù ở các mức độ khác nhau trên các phân ngành CNTT. Thứ hai, vị trí mạnh mẽ của Hoa Kỳ suy yếu tương đối so với các quốc gia khác ngày càng kết nối, đặc biệt là Trung Quốc. Trong khi Trung Quốc đã vượt qua Hoa Kỳ về tổng số bằng sáng chế trong lĩnh vực CNTT vào năm 2015, Trung Quốc hiện cũng đang bắt kịp từ góc độ mạng được thể hiện bằng vị trí trung tâm ngày càng tăng của mình trong giai đoạn thời gian quan sát."} +{"idx": 1870991, "en": "Significant association signals from genome-wide association studies (GWAS) point to genomic regions of interest. However, for most loci the causative genetic variant remains undefined. Determining expression quantitative trait loci (eQTL) in a disease relevant tissue is an excellent approach to zoom in on disease- or trait-associated association signals and hitherto on relevant disease mechanisms. To this end, we explored regulation of gene expression in healthy retina (n = 311) and generated the largest cis-eQTL data set available to date. Genotype- and RNA-Seq data underwent rigorous quality control protocols before FastQTL was applied to assess the influence of genetic markers on local (cis) gene expression. Our analysis identified 403,151 significant eQTL variants (eVariants) that regulate 3,007 genes (eGenes) (Q-Value < 0.05). A conditional analysis revealed 744 independent secondary eQTL signals for 598 of the 3,007 eGenes. Interestingly, 99,165 (24.71%) of all unique eVariants regulate the expression of more than one eGene. Filtering the dataset for eVariants regulating three or more eGenes revealed 96 potential regulatory clusters. Of these, 31 harbour 130 genes which are partially regulated by the same genetic signal. To correlate eQTL and association signals, GWAS data from twelve complex eye diseases or traits were included and resulted in identification of 80 eGenes with potential association. Remarkably, expression of 10 genes is regulated by eVariants associated with multiple eye diseases or traits. In conclusion, we generated a unique catalogue of gene expression regulation in healthy retinal tissue and applied this resource to identify potentially pleiotropic effects in highly prevalent human eye diseases. Our study provides an excellent basis to further explore mechanisms of various retinal disease etiologies.", "gemini": "Các tín hiệu liên kết đáng kể từ các nghiên cứu liên kết toàn bộ hệ gen (GWAS) chỉ ra các vùng gen quan tâm. Tuy nhiên, đối với hầu hết các gen, biến thể di truyền gây bệnh vẫn chưa được xác định. Việc xác định các gen định lượng tính trạng biểu hiện (eQTL) trong mô liên quan đến bệnh là một cách tiếp cận tuyệt vời để thu hẹp các tín hiệu liên kết liên quan đến bệnh hoặc tính trạng và cho đến nay là các cơ chế bệnh liên quan. Vì mục đích này, chúng tôi đã khám phá quy định về biểu hiện gen ở võng mạc khỏe mạnh (n = 311) và tạo ra bộ dữ liệu eQTL cis lớn nhất hiện có. Dữ liệu kiểu gen và RNA-Seq đã trải qua các giao thức kiểm soát chất lượng nghiêm ngặt trước khi FastQTL được áp dụng để đánh giá ảnh hưởng của các dấu hiệu di truyền lên biểu hiện gen cục bộ (cis). Phân tích của chúng tôi đã xác định 403.151 biến thể eQTL có ý nghĩa (eVariants) điều chỉnh 3.007 gen (eGenes) (Giá trị Q <0,05). Một phân tích có điều kiện đã tiết lộ 744 tín hiệu eQTL thứ cấp độc lập cho 598 trong số 3.007 eGenes. Điều thú vị là 99.165 (24,71%) trong số tất cả các eVariants duy nhất điều chỉnh biểu hiện của nhiều hơn một eGene. Việc lọc bộ dữ liệu đối với các eVariants điều chỉnh ba hoặc nhiều eGenes đã tiết lộ 96 cụm quy định tiềm ẩn. Trong số đó, 31 chứa 130 gen được điều chỉnh một phần bởi cùng một tín hiệu di truyền. Để tương quan tín hiệu eQTL và tín hiệu liên kết, dữ liệu GWAS từ mười hai bệnh hoặc tính trạng về mắt phức tạp đã được đưa vào và dẫn đến việc xác định 80 eGenes có liên kết tiềm ẩn. Đáng chú ý, biểu hiện của 10 gen được điều chỉnh bởi các eVariants liên quan đến nhiều bệnh hoặc tính trạng về mắt. Tóm lại, chúng tôi đã tạo ra một danh mục duy nhất về quy định biểu hiện gen trong mô võng mạc khỏe mạnh và áp dụng nguồn tài nguyên này để xác định các tác động đa hình tiềm ẩn ở các bệnh về mắt phổ biến ở người. Nghiên cứu của chúng tôi cung cấp một cơ sở tuyệt vời để khám phá sâu hơn các cơ chế của nhiều nguyên nhân gây bệnh võng mạc."} +{"idx": 1870992, "en": "This systematic review aims to summarize the evidence regarding the effectiveness of interventions targeting energy balance-related behaviors in children from lower socioeconomic environments and the applied behavior change techniques. The literature search was conducted in Cochrane, Embase, Psycinfo and Pubmed. Articles had to be published between January 2000 and September 2019. Studies were included that i) targeted dietary behavior, physical activity and/or sedentary behavior; ii) had a controlled trial design; iii) included children aged 9-12 years old; iv) focused on lower socioeconomic environments; and v) took place in upper-middle or high income countries. Two independent researchers extracted data, identified behavior change techniques using the Behavior Change Technique Taxonomy v1, and performed a methodological quality assessment using the quality assessment tool of the Effective Public Health Practice Project. We included 24 studies, of which one received a high and three a moderate quality rating. Demonstration, practice and providing instructions on how to perform a behavior were the most commonly applied behavior change techniques. Seven studies reported significant beneficial intervention effects: five on physical activity, one on physical activity and sedentary behavior and one on dietary behavior. When comparing effective versus non-effective interventions, and comparing our review to previous reviews focusing on children from the general population, similar behavior change techniques were applied. More high quality research is needed to evaluate the effectiveness of interventions and their behavior change techniques targeting children of low socioeconomic environments. PROSPERO registration number: CRD42016052599.", "gemini": "Mục tiêu của bài tổng quan hệ thống này là tóm tắt các bằng chứng về hiệu quả của các can thiệp nhắm vào các hành vi liên quan đến cân bằng năng lượng ở trẻ em từ các môi trường kinh tế xã hội thấp hơn và các kỹ thuật thay đổi hành vi được áp dụng. Việc tìm kiếm tài liệu đã được thực hiện trong Cochrane, Embase, Psycinfo và Pubmed. Các bài báo phải được xuất bản trong khoảng thời gian từ tháng 1 năm 2000 đến tháng 9 năm 2019. Các nghiên cứu được đưa vào là các nghiên cứu i) nhắm vào hành vi ăn uống, hoạt động thể chất và/hoặc hành vi ít vận động; ii) có thiết kế thử nghiệm có đối chứng; iii) bao gồm trẻ em từ 9-12 tuổi; iv) tập trung vào các môi trường kinh tế xã hội thấp hơn; và v) diễn ra ở các quốc gia có thu nhập trung bình cao hoặc cao. Hai nhà nghiên cứu độc lập đã trích xuất dữ liệu, xác định các kỹ thuật thay đổi hành vi bằng cách sử dụng Phân loại kỹ thuật thay đổi hành vi v1 và thực hiện đánh giá chất lượng phương pháp luận bằng cách sử dụng công cụ đánh giá chất lượng của Dự án thực hành y tế công cộng hiệu quả. Chúng tôi đã đưa vào 24 nghiên cứu, trong đó có một nghiên cứu được đánh giá chất lượng cao và ba nghiên cứu được đánh giá chất lượng trung bình. Trình diễn, thực hành và hướng dẫn cách thực hiện một hành vi là những kỹ thuật thay đổi hành vi được áp dụng phổ biến nhất. Bảy nghiên cứu báo cáo các tác động can thiệp có lợi đáng kể: năm nghiên cứu về hoạt động thể chất, một nghiên cứu về hoạt động thể chất và hành vi ít vận động và một nghiên cứu về hành vi ăn uống. Khi so sánh các can thiệp hiệu quả so với không hiệu quả và so sánh bài tổng quan của chúng tôi với các bài tổng quan trước đây tập trung vào trẻ em trong cộng đồng nói chung, các kỹ thuật thay đổi hành vi tương tự đã được áp dụng. Cần nhiều nghiên cứu chất lượng cao hơn để đánh giá hiệu quả của các can thiệp và các kỹ thuật thay đổi hành vi của chúng nhắm vào trẻ em có hoàn cảnh kinh tế xã hội thấp. Số đăng ký PROSPERO: CRD42016052599."} +{"idx": 1870993, "en": "Changing institutional culture to be more diverse and inclusive within the biomedical academic community is difficult for many reasons. Herein we present evidence that a collaborative model involving multiple institutions of higher education can initiate and execute individual institutional change directed at enhancing diversity and inclusion at the postdoctoral researcher (postdoc) and junior faculty level by implementing evidence-based mentoring practices. A higher education consortium, the Big Ten Academic Alliance, invited individual member institutions to send participants to one of two types of annual mentor training: 1) \"Mentoring-Up\" training for postdocs, a majority of whom were from underrepresented groups; 2) Mentor Facilitator training-a train-the-trainer model-for faculty and senior leadership. From 2016 to 2019, 102 postdocs and 160 senior faculty and administrative leaders participated. Postdocs reported improvements in their mentoring proficiency (87%) and improved relationships with their PIs (71%). 29% of postdoc respondents transitioned to faculty positions, and 85% of these were underrepresented and 75% were female. 59 out of the 120 faculty and administrators (49%) trained in the first three years provided mentor training on their campuses to over 3000 undergraduate and graduate students, postdocs and faculty within the project period. We conclude that early stage biomedical professionals as well as individual institutions of higher education benefited significantly from this collaborative mentee/mentor training model.", "gemini": "Việc thay đổi văn hóa của tổ chức để trở nên đa dạng và toàn diện hơn trong cộng đồng học thuật y sinh là điều khó khăn vì nhiều lý do. Trong bài viết này, chúng tôi trình bày bằng chứng cho thấy một mô hình hợp tác liên quan đến nhiều tổ chức giáo dục đại học có thể khởi xướng và thực hiện thay đổi thể chế riêng lẻ nhằm tăng cường tính đa dạng và toàn diện ở cấp độ nghiên cứu sinh sau tiến sĩ (postdoc) và giảng viên cơ hữu bằng cách thực hiện các hoạt động cố vấn dựa trên bằng chứng. Một liên minh giáo dục đại học, Liên minh học thuật Big Ten, đã mời các tổ chức thành viên riêng lẻ cử người tham gia một trong hai loại đào tạo cố vấn thường niên: 1) Đào tạo \"Cố vấn\" dành cho các nghiên cứu sinh sau tiến sĩ, phần lớn trong số họ đến từ các nhóm chưa được đại diện; 2) Đào tạo Cố vấn đào tạo-một mô hình đào tạo người hướng dẫn-dành cho giảng viên và lãnh đạo cấp cao. Từ năm 2016 đến năm 2019, có 102 nghiên cứu sinh sau tiến sĩ và 160 giảng viên cao cấp và lãnh đạo hành chính đã tham gia. Các nghiên cứu sinh sau tiến sĩ đã báo cáo về sự cải thiện về năng lực cố vấn của họ (87%) và cải thiện mối quan hệ với PI của họ (71%). 29% số nghiên cứu sinh sau tiến sĩ được hỏi đã chuyển sang các vị trí giảng viên, và 85% trong số họ chưa được đại diện và 75% là nữ. 59 trong số 120 giảng viên và quản trị viên (49%) được đào tạo trong ba năm đầu tiên đã cung cấp đào tạo cố vấn tại khuôn viên trường của họ cho hơn 3000 sinh viên đại học và sau đại học, nghiên cứu sinh sau tiến sĩ và giảng viên trong thời gian của dự án. Chúng tôi kết luận rằng các chuyên gia y sinh học giai đoạn đầu cũng như các tổ chức giáo dục đại học riêng lẻ đã được hưởng lợi đáng kể từ mô hình đào tạo người được cố vấn/cố vấn hợp tác này."} +{"idx": 1870994, "en": "BACKGROUND Dissemination of accurate health research information to patients and families has become increasingly important with the rise of the internet as a means of finding health information. However, the public faces several barriers to accessing research information, including paywalls and technical jargon. One method to bridge this gap between patients, families, and research is using lay summaries. SCAsource is an online knowledge translation platform where peer-reviewed research papers on ataxia are translated into lay summaries. This online platform was launched in September 2018, with the goal of making ataxia research more accessible and understandable to patients and families. A secondary goal is to provide opportunities for ataxia researchers to develop and hone their knowledge translation skills, altogether improving the quality of patient communication in the ataxia community. AIM The aim of this study was to measure the impact of SCAsource on its readers and volunteer contributors after one year of activity. This is to ensure SCAsource is meeting its goals of (1) improving access and understanding of ataxia research to lay audiences, and (2) improving knowledge translation skills of volunteer contributors. METHODS Two online surveys were launched, one for readers and one for volunteers. Each survey had a combination of multiple-choice, Likert-scale type, and open-ended short-answer questions. Descriptive quantitative analysis was used for respondent characteristics and Likert-type data. A grounded theory coding approach was used to analyze narrative feedback data. RESULTS We found that SCAsource has mutually beneficial outcomes for both lay person readers and volunteer contributors. Readers have an increased understanding of ataxia research and access to up-to-date information on recent publications. Volunteers develop knowledge translation skills and have increased confidence in communicating results to lay audiences. Areas of improvement were identified to be incorporated into the platform. CONCLUSION We demonstrated that SCAsource improves access to information and understanding of research to lay audiences, while providing opportunities for researchers to develop knowledge translation skills. This framework can potentially be used by other rare disease organizations to launch and evaluate their own knowledge translation websites.", "gemini": "BỐI CẢNH Việc phổ biến thông tin nghiên cứu sức khỏe chính xác đến bệnh nhân và gia đình ngày càng trở nên quan trọng khi internet trở thành phương tiện tìm kiếm thông tin sức khỏe. Tuy nhiên, công chúng phải đối mặt với một số rào cản khi tiếp cận thông tin nghiên cứu, bao gồm tường phí và thuật ngữ kỹ thuật. Một phương pháp để thu hẹp khoảng cách này giữa bệnh nhân, gia đình và nghiên cứu là sử dụng các bản tóm tắt dành cho người dân thường. SCAsource là một nền tảng chuyển giao kiến thức trực tuyến, nơi các bài báo nghiên cứu được bình duyệt về chứng mất điều hòa được dịch thành các bản tóm tắt dành cho người dân thường. Nền tảng trực tuyến này được ra mắt vào tháng 9 năm 2018, với mục tiêu làm cho nghiên cứu về chứng mất điều hòa dễ tiếp cận và dễ hiểu hơn đối với bệnh nhân và gia đình. Một mục tiêu thứ cấp là cung cấp cơ hội cho các nhà nghiên cứu về chứng mất điều hòa phát triển và trau dồi các kỹ năng chuyển giao kiến thức của họ, cùng nhau cải thiện chất lượng giao tiếp với bệnh nhân trong cộng đồng chứng mất điều hòa. MỤC ĐÍCH Mục đích của nghiên cứu này là đo lường tác động của SCAsource đối với độc giả và những người đóng góp tình nguyện sau một năm hoạt động. Điều này nhằm đảm bảo SCAsource đang đạt được các mục tiêu của mình là (1) cải thiện khả năng tiếp cận và hiểu biết về nghiên cứu chứng mất điều hòa cho đối tượng là người dân thường và (2) cải thiện các kỹ năng chuyển giao kiến thức của những người đóng góp tình nguyện. PHƯƠNG PHÁP Hai cuộc khảo sát trực tuyến đã được đưa ra, một dành cho độc giả và một dành cho tình nguyện viên. Mỗi cuộc khảo sát có sự kết hợp giữa các câu hỏi trắc nghiệm, kiểu thang Likert và các câu hỏi trả lời ngắn mở. Phân tích định lượng mô tả được sử dụng cho các đặc điểm của người trả lời và dữ liệu kiểu Likert. Một phương pháp mã hóa lý thuyết cơ bản được sử dụng để phân tích dữ liệu phản hồi tường thuật. KẾT QUẢ Chúng tôi thấy rằng SCAsource có kết quả có lợi cho cả độc giả là người dân thường và những người đóng góp tình nguyện. Độc giả hiểu biết hơn về nghiên cứu chứng mất điều hòa và có thể tiếp cận thông tin cập nhật về các ấn phẩm gần đây. Những người tình nguyện phát triển các kỹ năng chuyển giao kiến thức và tự tin hơn trong việc truyền đạt kết quả cho đối tượng là người dân thường. Các lĩnh vực cần cải thiện đã được xác định để đưa vào nền tảng. KẾT LUẬN Chúng tôi đã chứng minh rằng SCAsource cải thiện khả năng tiếp cận thông tin và hiểu biết về nghiên cứu cho đối tượng là người dân thường, đồng thời tạo cơ hội cho các nhà nghiên cứu phát triển các kỹ năng chuyển giao kiến thức. Khung này có khả năng được các tổ chức bệnh hiếm khác sử dụng để ra mắt và đánh giá các trang web chuyển giao kiến thức của riêng họ."} +{"idx": 1870995, "en": "[This corrects the article DOI: 10.1371/journal.ppat.1002558.].", "gemini": "[Điều này sửa lỗi cho bài báo DOI: 10.1371/journal.ppat.1002558.]."} +{"idx": 1870996, "en": "Bone scaffolds are widely used as one of the main bone substitute materials. However, many bone scaffold microstructure topologies exist and it is still unclear which topology to use when designing scaffold for a specific application. The aim of the present study was to reveal the mechanism of the microstructure-driven performance of bone scaffold and thus to provide guideline on scaffold design. Finite element (FE) models of five TPMS (Diamond, Gyroid, Schwarz P, Fischer-Koch S and F-RD) and three traditional (Cube, FD-Cube and Octa) scaffolds were generated. The effective compressive and shear moduli of scaffolds were calculated from the mechanical analysis using the FE unit cell models with the periodic boundary condition. The scaffold permeability was calculated from the computational fluid dynamics (CFD) analysis using the 4×4×4 FE models. It is revealed that the surface-to-volume ratio of the Fischer-Koch S-based scaffold is the highest among the scaffolds investigated. The mechanical analysis revealed that the bending deformation dominated structures (e.g., the Diamond, the Gyroid, the Schwarz P) have higher effective shear moduli. The stretching deformation dominated structures (e.g., the Schwarz P, the Cube) have higher effective compressive moduli. For all the scaffolds, when the same amount of change in scaffold porosity is made, the corresponding change in the scaffold relative shear modulus is larger than that in the relative compressive modulus. The CFD analysis revealed that the structures with the simple and straight pores (e.g., Cube) have higher permeability than the structures with the complex pores (e.g., Fischer-Koch S). The main contribution of the present study is that the relationship between scaffold properties and the underlying microstructure is systematically investigated and thus some guidelines on the design of bone scaffolds are provided, for example, in the scenario where a high surface-to-volume ratio is required, it is suggested to use the Fischer-Koch S based scaffold.", "gemini": "Giàn xương xương được sử dụng rộng rãi như là một trong những vật liệu thay thế xương chính. Tuy nhiên, có nhiều cấu trúc vi mô giàn xương và vẫn chưa rõ cấu trúc nào nên được sử dụng khi thiết kế giàn xương cho một ứng dụng cụ thể. Mục đích của nghiên cứu hiện tại là để khám phá cơ chế hiệu suất do cấu trúc vi mô của giàn xương và do đó cung cấp hướng dẫn về thiết kế giàn xương. Các mô hình phần tử hữu hạn (FE) của năm TPMS (Kim cương, Gyroid, Schwarz P, Fischer-Koch S và F-RD) và ba giàn xương truyền thống (Lập phương, FD-Lập phương và Bát diện) đã được tạo ra. Mô đun nén và cắt hiệu quả của giàn xương được tính toán từ phân tích cơ học sử dụng các mô hình ô đơn vị FE với điều kiện biên giới tuần hoàn. Độ thấm của giàn xương được tính toán từ phân tích động lực học chất lỏng tính toán (CFD) sử dụng các mô hình FE 4 × 4 × 4. Kết quả cho thấy tỷ lệ diện tích bề mặt trên thể tích của giàn xương dựa trên Fischer-Koch S là cao nhất trong số các giàn xương được nghiên cứu. Phân tích cơ học cho thấy các cấu trúc biến dạng uốn cong chiếm ưu thế (ví dụ: Kim cương, Gyroid, Schwarz P) có mô đun cắt hiệu quả cao hơn. Các cấu trúc biến dạng kéo dài chiếm ưu thế (ví dụ: Schwarz P, Lập phương) có mô đun nén hiệu quả cao hơn. Đối với tất cả các giàn xương, khi thực hiện cùng một lượng thay đổi độ xốp của giàn xương, thì sự thay đổi tương ứng trong mô đun cắt tương đối của giàn xương lớn hơn sự thay đổi trong mô đun nén tương đối. Phân tích CFD cho thấy các cấu trúc có các lỗ rỗng đơn giản và thẳng (ví dụ: Lập phương) có độ thấm cao hơn các cấu trúc có các lỗ rỗng phức tạp (ví dụ: Fischer-Koch S). Đóng góp chính của nghiên cứu hiện tại là mối quan hệ giữa các đặc tính giàn xương và cấu trúc vi mô cơ bản được nghiên cứu một cách có hệ thống và do đó một số hướng dẫn về thiết kế giàn xương được cung cấp, ví dụ, trong trường hợp cần tỷ lệ diện tích bề mặt trên thể tích cao, thì nên sử dụng giàn xương dựa trên Fischer-Koch S."} +{"idx": 1870997, "en": "Dendrobium bibenzyls and phenanthrenes such as chrysotoxine, cypripedin, gigantol and moscatilin have been reported to show promising inhibitory effects on lung cancer growth and metastasis in ex vivo human cell line models, suggesting their potential for clinical application in patients with lung cancer. However, it remains to be determined whether these therapeutic effects can be also seen in primary human cells and/or in vivo. In this study, we comparatively investigated the immune modulatory effects of bibenzyls and phenanthrenes, including a novel Dendrobium bibenzyl derivative, in primary human monocytes. All compounds were isolated and purified from a Thai orchid Dendrobium lindleyi Steud, a new source of therapeutic compounds with promising potential of tissue culture production. We detected increased frequencies of TNF- and IL-6-expressing monocytes after treatment with gigantol and cypripedin, whereas chrysotoxine and moscatilin did not alter the expression of these cytokines in monocytes. Interestingly, the new 4,5-dihydroxy-3,3',4'-trimethoxybibenzyl derivative showed dose-dependent immune modulatory effects in lipopolysaccharide (LPS)-treated CD14lo and CD14hi monocytes. Together, our findings show immune modulatory effects of the new bibenzyl derivative from Dendrobium lindleyi on different monocyte sub-populations. However, therapeutic consequences of these different monocyte populations on human diseases including cancer remain to be investigated.", "gemini": "Bibenzyl và phenanthrene của Dendrobium như chrysotoxine, cypripedin, gigantol và moscatilin đã được báo cáo là có tác dụng ức chế đáng hứa hẹn đối với sự phát triển và di căn của ung thư phổi trong các mô hình dòng tế bào người ngoại sinh, cho thấy tiềm năng ứng dụng lâm sàng ở những bệnh nhân ung thư phổi. Tuy nhiên, vẫn cần xác định xem các tác dụng điều trị này có thể được thấy ở các tế bào người nguyên phát và/hoặc trong cơ thể sống hay không. Trong nghiên cứu này, chúng tôi đã so sánh các tác dụng điều hòa miễn dịch của bibenzyl và phenanthrene, bao gồm cả một dẫn xuất bibenzyl mới của Dendrobium, ở các tế bào đơn nhân người nguyên phát. Tất cả các hợp chất đều được phân lập và tinh chế từ một loài lan Thái Lan Dendrobium lindleyi Steud, một nguồn hợp chất điều trị mới có tiềm năng sản xuất nuôi cấy mô hứa hẹn. Chúng tôi phát hiện thấy tần số tăng của các tế bào đơn nhân biểu hiện TNF và IL-6 sau khi điều trị bằng gigantol và cypripedin, trong khi chrysotoxine và moscatilin không làm thay đổi biểu hiện của các cytokine này ở các tế bào đơn nhân. Thật thú vị, dẫn xuất 4,5-dihydroxy-3,3',4'-trimethoxybibenzyl mới cho thấy các tác dụng điều hòa miễn dịch phụ thuộc vào liều ở các tế bào đơn nhân CD14lo và CD14hi được xử lý bằng lipopolysaccharide (LPS). Cùng nhau, những phát hiện của chúng tôi cho thấy tác dụng điều hòa miễn dịch của dẫn xuất bibenzyl mới từ Dendrobium lindleyi trên các quần thể đơn nhân khác nhau. Tuy nhiên, hậu quả điều trị của các quần thể đơn nhân khác nhau này đối với các bệnh của con người bao gồm cả ung thư vẫn cần được nghiên cứu."} +{"idx": 1870998, "en": "Gender-wage gaps are an important phenomenon on labor markets. They can possibly be caused by the institutional framework. This question is addressed in this paper. When only joint output can be observed in team production, individuals may submit self-reports of their contribution to a principal. In a multi-employee gift exchange experiment, we study how men and women behave differently with and without such self-reports. We cannot reject that self-reports left the overall efficiency of the gift exchange interaction unchanged, but detect notable gender differences. Women reported similar effort levels as men, but contributed significantly less. The difference in contributions led to a significant gender gap in wages, depending on gender group composition. These effects were only present when participants did not know each other's gender, however. When instead gender was observable, the behavior of men and women converged. The results suggest that parts of wage gaps may be related to different behavior within incomplete contract and imperfect information environments, depending on details of the informational context.", "gemini": "Khoảng cách tiền lương theo giới là một hiện tượng quan trọng trên thị trường lao động. Chúng có thể có thể do khuôn khổ thể chế gây ra. Câu hỏi này được giải quyết trong bài báo này. Khi chỉ có thể quan sát được sản lượng chung trong sản xuất theo nhóm, các cá nhân có thể gửi báo cáo tự đánh giá về đóng góp của họ cho một hiệu trưởng. Trong một thí nghiệm trao đổi quà tặng của nhiều nhân viên, chúng tôi nghiên cứu cách nam giới và phụ nữ cư xử khác nhau có và không có các báo cáo tự đánh giá như vậy. Chúng tôi không thể bác bỏ rằng các báo cáo tự đánh giá đã không thay đổi hiệu quả chung của tương tác trao đổi quà tặng, nhưng phát hiện ra sự khác biệt đáng kể giữa các giới. Phụ nữ báo cáo mức độ nỗ lực tương tự như nam giới, nhưng đóng góp ít hơn đáng kể. Sự khác biệt về đóng góp dẫn đến khoảng cách tiền lương đáng kể giữa các giới, tùy thuộc vào thành phần nhóm giới. Tuy nhiên, những tác động này chỉ có mặt khi những người tham gia không biết giới tính của nhau. Tuy nhiên, khi giới tính có thể quan sát được, hành vi của nam giới và phụ nữ lại hội tụ. Kết quả cho thấy rằng một phần của khoảng cách tiền lương có thể liên quan đến hành vi khác nhau trong môi trường hợp đồng không đầy đủ và thông tin không hoàn hảo, tùy thuộc vào các chi tiết của bối cảnh thông tin."} +{"idx": 1870999, "en": "Highly exposed seronegative (HESN) individuals present a unique setting to study mechanisms of protection against HIV acquisition. As natural killer (NK) cell activation and function have been implicated as a correlate of protection in HESN individuals, we sought to better understand the features of NK cells that may confer protection. We used mass cytometry to phenotypically profile NK cells from a cohort of Beninese sex workers and healthy controls. We found that NK cells from HESN women had increased expression of NKG2A, NKp30 and LILRB1, as well as the Fc receptor CD16, and decreased expression of DNAM-1, CD94, Siglec-7, and NKp44. Using functional assessments of NK cells from healthy donors against autologous HIV-infected CD4+ T cells, we observed that NKp30+ and Siglec-7+ cells had improved functional activity. Further, we found that NK cells from HESN women trended towards increased antibody-dependent cellular cytotoxicity (ADCC) activity; this activity correlated with increased CD16 expression. Overall, we identify features of NK cells in HESN women that may contribute to protection from HIV infection. Follow up studies with larger cohorts are warranted to confirm these findings.", "gemini": "Những cá nhân huyết thanh âm tính có phơi nhiễm cao (HESN) đưa ra một bối cảnh duy nhất để nghiên cứu cơ chế bảo vệ chống lại việc mắc HIV. Vì hoạt động và chức năng của tế bào giết tự nhiên (NK) đã được chứng minh là tương quan với khả năng bảo vệ ở những cá nhân HESN, chúng tôi đã tìm cách hiểu rõ hơn các đặc điểm của tế bào NK có thể mang lại khả năng bảo vệ. Chúng tôi đã sử dụng phép đo khối lượng tế bào để lập hồ sơ kiểu hình tế bào NK từ một nhóm đối tượng là những người hành nghề mại dâm ở Benin và những người đối chứng khỏe mạnh. Chúng tôi thấy rằng các tế bào NK từ những phụ nữ HESN có biểu hiện tăng NKG2A, NKp30 và LILRB1, cũng như thụ thể Fc CD16 và biểu hiện giảm DNAM-1, CD94, Siglec-7 và NKp44. Sử dụng các đánh giá chức năng của các tế bào NK từ những người hiến tặng khỏe mạnh so với các tế bào T CD4+ bị nhiễm HIV tự thân, chúng tôi thấy rằng các tế bào NKp30+ và Siglec-7+ có hoạt động chức năng được cải thiện. Hơn nữa, chúng tôi thấy rằng các tế bào NK từ những phụ nữ HESN có xu hướng tăng hoạt động tế bào phụ thuộc kháng thể (ADCC); hoạt động này tương quan với biểu hiện CD16 tăng lên. Nhìn chung, chúng tôi xác định các đặc điểm của tế bào NK ở những phụ nữ HESN có thể góp phần bảo vệ khỏi nhiễm HIV. Các nghiên cứu tiếp theo với các nhóm đối tượng lớn hơn được bảo hành để xác nhận những phát hiện này."} +{"idx": 1871000, "en": "The proposed method is to do simplification for Digital Elevation Model (DEM), which uses a few of original nodes representing the terrain surface while maintaining the accuracy. The original DEM nodes are sampled using the Maximal Poisson-disk Sampling (MPS), in which, the disk's size of each sample is computed on basis of the Singular Value Decomposition (SVD). MPS can generate the hyper-uniformly distributed samples and was taken to do DEM adaptive sampling by being combined with the geodesic metric. However, the geodesic distance computation is complex and the requirement for memory is high. As such, this paper proposes an extension of the classic MPS based method for selecting quasi-randomly distributed points from DEM nodes based on the distribution of eigenvalues, accounting for surface heterogeneity. To achieve this objective, uniform MPS is conducted to sample the DEM nodes by setting the related disk radius to be inversely proportional to the local terrain complexity, which is defined as an index expressing the local terrain variation. Then, the geodesic metric related parameters are implicitly contained in the defined index. As a result, more samples are concentrated in the rugged regions, and vice versa. The proposed method shows better perfermance, at least the results are comparable with the geodesic distance based Poisson disk sampling method. Meanwhile, it greatly accelerates the sampling process and reduces the memory cost.", "gemini": "Phương pháp được đề xuất là thực hiện đơn giản hóa cho Mô hình độ cao kỹ thuật số (DEM), sử dụng một số ít các nút ban đầu đại diện cho bề mặt địa hình trong khi vẫn duy trì độ chính xác. Các nút DEM ban đầu được lấy mẫu bằng Phép lấy mẫu đĩa Poisson cực đại (MPS), trong đó, kích thước đĩa của mỗi mẫu được tính toán trên cơ sở Phân tích giá trị kỳ dị (SVD). MPS có thể tạo ra các mẫu phân bố siêu đồng đều và được sử dụng để lấy mẫu thích ứng DEM bằng cách kết hợp với phép đo địa lý. Tuy nhiên, phép tính khoảng cách địa lý rất phức tạp và yêu cầu về bộ nhớ rất cao. Do đó, bài báo này đề xuất một phần mở rộng của phương pháp dựa trên MPS cổ điển để chọn các điểm phân bố ngẫu nhiên từ các nút DEM dựa trên phân phối các giá trị riêng, tính đến tính không đồng nhất của bề mặt. Để đạt được mục tiêu này, MPS thống nhất được tiến hành để lấy mẫu các nút DEM bằng cách đặt bán kính đĩa liên quan tỷ lệ nghịch với độ phức tạp của địa hình cục bộ, được định nghĩa là chỉ số biểu thị sự thay đổi của địa hình cục bộ. Sau đó, các thông số liên quan đến phép đo địa lý được bao hàm ngầm trong chỉ số được xác định. Kết quả là, nhiều mẫu hơn tập trung ở các vùng gồ ghề, và ngược lại. Phương pháp được đề xuất cho thấy hiệu suất tốt hơn, ít nhất là các kết quả có thể so sánh với phương pháp lấy mẫu đĩa Poisson dựa trên khoảng cách địa lý. Trong khi đó, nó đẩy nhanh đáng kể quá trình lấy mẫu và giảm chi phí bộ nhớ."} +{"idx": 1871001, "en": "Despite rapid macro-economic growth, one-third of the global burden of childhood stunting is contributed by India. This burden is characterized by wide-spread geographical variation within the country. This paper explores two research questions: (i) are the drivers of severe and moderate stunting similar? (ii) differential endowments or policy-effect, how do community-level nutrition and sanitary practices affect inter-state differences? Using data from Indian National Family and Health Survey 4, 2015-16, six states holding different ranks in the stunting continuum are compared to Tamil Nadu, taken as the benchmark state due to its laudable performance in the health care sector. Applying quantile regression approaches, the difference in state-level performance is decomposed into detailed covariate effects (differential endowments) and coefficient effects (differential strength of association between the drivers and outcome). The explanatory variables are not similarly associated with severe and moderate stunting. Decomposition results demonstrate a significant role of community-level sanitation practices compared to child nutrition behaviour in explaining the inter-state disparity. Coefficient effects play a dominant role in the lower tail of HAZ distribution for the poor performing states indicating that the worse outcomes of these states are due to weaker policy effects of the control variables on stunting. Multi-sectoral approach, identification and differentiation between severe and moderate stunting cases can be more instrumental in managing and reducing the scourge. This paper also advocates the potential benefits of customizing centrally-launched policies as per the state's performance and introducing the concept coproduction in the existing nutrition and health policy framework. This will instigate a feeling of ownership of the problem of childhood stunting among the policy consumers and strengthen the influence of policies on the outcomes.", "gemini": "Mặc dù tăng trưởng kinh tế vĩ mô nhanh chóng, Ấn Độ đóng góp một phần ba gánh nặng toàn cầu về tình trạng thấp còi ở trẻ em. Gánh nặng này được đặc trưng bởi sự khác biệt về mặt địa lý rộng rãi trong cả nước. Bài báo này khám phá hai câu hỏi nghiên cứu: (i) các động lực của tình trạng thấp còi nghiêm trọng và trung bình có giống nhau không? (ii) sự khác biệt về nguồn lực hoặc hiệu quả chính sách, các hoạt động dinh dưỡng và vệ sinh ở cấp cộng đồng ảnh hưởng đến sự khác biệt giữa các tiểu bang như thế nào? Sử dụng dữ liệu từ Khảo sát Sức khỏe và Gia đình Quốc gia Ấn Độ 4, 2015-16, sáu tiểu bang giữ các thứ hạng khác nhau trong tình trạng thấp còi liên tục được so sánh với Tamil Nadu, được coi là tiểu bang chuẩn mực do thành tích đáng khen ngợi trong lĩnh vực chăm sóc sức khỏe. Áp dụng các phương pháp hồi quy phân vị, sự khác biệt về hiệu suất cấp tiểu bang được phân tích thành các hiệu ứng biến số chi tiết (sự khác biệt về nguồn lực) và hiệu ứng hệ số (sức mạnh liên kết khác nhau giữa các động lực và kết quả). Các biến giải thích không liên quan tương tự đến tình trạng thấp còi nghiêm trọng và trung bình. Kết quả phân tích cho thấy vai trò quan trọng của các hoạt động vệ sinh ở cấp cộng đồng so với hành vi dinh dưỡng của trẻ em trong việc giải thích sự chênh lệch giữa các tiểu bang. Hiệu ứng hệ số đóng vai trò chủ đạo trong phần đuôi dưới của phân phối HAZ đối với các tiểu bang có hiệu suất kém, cho thấy rằng kết quả tồi tệ hơn của các tiểu bang này là do hiệu ứng chính sách yếu hơn của các biến kiểm soát đối với tình trạng thấp còi. Tiếp cận đa ngành, xác định và phân biệt giữa các trường hợp thấp còi nghiêm trọng và trung bình có thể hữu ích hơn trong việc quản lý và giảm tình trạng này. Bài báo này cũng ủng hộ những lợi ích tiềm năng của việc tùy chỉnh các chính sách do trung ương ban hành theo hiệu suất của tiểu bang và đưa ra khái niệm đồng sản xuất vào khuôn khổ chính sách dinh dưỡng và sức khỏe hiện hành. Điều này sẽ thúc đẩy cảm giác sở hữu vấn đề thấp còi ở trẻ em trong số những người tiêu dùng chính sách và tăng cường ảnh hưởng của các chính sách đối với kết quả."} +{"idx": 1871002, "en": "This paper presents an improved method to estimate the blur parameters of motion deblurring algorithm for single image restoration based on the point spread function (PSF) in frequency spectrum. We then introduce a modification to the Radon transform in the blur angle estimation scheme with our proposed difference value vs angle curve. Subsequently, the auto-correlation matrix is employed to estimate the blur angle by measuring the distance between the conjugated-correlated troughs. Finally, we evaluate the accuracy, robustness and time efficiency of our proposed method with the existing algorithms on the public benchmarks and the natural real motion blurred images. The experimental results demonstrate that the proposed PSF estimation scheme not only could obtain a higher accuracy for the blur angle and blur length, but also demonstrate stronger robustness and higher time efficiency under different circumstances.", "gemini": "Bài báo này trình bày một phương pháp cải tiến để ước tính các tham số làm mờ của thuật toán khử mờ chuyển động để khôi phục hình ảnh đơn dựa trên hàm điểm lan truyền (PSF) trong phổ tần số. Sau đó, chúng tôi giới thiệu một sửa đổi đối với phép biến đổi Radon trong lược đồ ước tính góc mờ với đường cong giá trị chênh lệch so với góc mà chúng tôi đề xuất. Sau đó, ma trận tự tương quan được sử dụng để ước tính góc mờ bằng cách đo khoảng cách giữa các máng tương quan liên hợp. Cuối cùng, chúng tôi đánh giá độ chính xác, độ mạnh và hiệu quả về thời gian của phương pháp chúng tôi đề xuất với các thuật toán hiện có trên các chuẩn mực công khai và các hình ảnh bị mờ chuyển động tự nhiên thực tế. Kết quả thử nghiệm chứng minh rằng lược đồ ước tính PSF được đề xuất không chỉ có thể đạt được độ chính xác cao hơn đối với góc mờ và độ dài mờ, mà còn thể hiện độ mạnh hơn và hiệu quả về thời gian cao hơn trong các trường hợp khác nhau."} +{"idx": 1871003, "en": "BACKGROUND Cardiovascular disease (CVD) is the leading cause of death in the United States and African Americans (AA) have a disproportionately greater burden of CVD as compared to Whites. The American Heart Association (AHA) Life's Simple 7 (LS7) framework outlines goals for attaining ideal cardiovascular health. Yet, there is a lack of evidence summarizing best practices to maximize LS7 attainment. The objective of the present study was to systematically review the extant peer-reviewed literature on community-engaged and community-based participatory research (CBPR) aimed at improving one or more LS7 metrics among AA. METHODS PubMed, CINAHL, and Embase databases were searched. We included articles that reported quantitative results for one or more of the following LS7 metrics: physical activity, diet, cholesterol, blood pressure, body mass index, smoking, and glycemia. We included analyses with a greater than 50% AA study population focused on adults (≥18 years of age). RESULTS Of the 1008 unique studies identified, 54 met inclusion criteria; 27 of which were randomized controlled trials. 50% of studies assessed more than one LS7 metric but only two studies evaluated all seven of the LS7 metrics. No studies had a high proportion of AA males. 40 studies improved at least one LS7 metric at the study end-point. Formative research was used in many studies to guide intervention design. Studies were of varying quality, but overall rated \"fair\" using a modified approach to the National Institute of Health quality assessment tool. CONCLUSION There is insufficient data to recommend a specific community-engaged or CBPR intervention to improve attainment of LS7 metrics among AA. Future studies using rigorous methodology with increased gender diversity and utilizing the AHA LS7 framework are required to establish a validated program to improve LS7 in AAs.", "gemini": "Bối cảnh Bệnh tim mạch (CVD) là nguyên nhân gây tử vong hàng đầu tại Hoa Kỳ và người Mỹ gốc Phi (AA) có gánh nặng CVD lớn hơn nhiều so với người da trắng. Khung Life's Simple 7 (LS7) của Hiệp hội Tim mạch Hoa Kỳ (AHA) nêu ra các mục tiêu để đạt được sức khỏe tim mạch lý tưởng. Tuy nhiên, vẫn thiếu bằng chứng tóm tắt các thông lệ tốt nhất để tối đa hóa việc đạt được LS7. Mục tiêu của nghiên cứu hiện tại là xem xét một cách có hệ thống các tài liệu được bình duyệt hiện có về nghiên cứu tham gia cộng đồng và dựa trên cộng đồng (CBPR) nhằm cải thiện một hoặc nhiều số liệu LS7 trong số AA. Phương pháp Đã tìm kiếm các cơ sở dữ liệu PubMed, CINAHL và Embase. Chúng tôi đã đưa vào các bài báo báo cáo kết quả định lượng cho một hoặc nhiều số liệu LS7 sau: hoạt động thể chất, chế độ ăn uống, cholesterol, huyết áp, chỉ số khối cơ thể, hút thuốc và đường huyết. Chúng tôi đã đưa vào các phân tích có hơn 50% dân số nghiên cứu là người Mỹ gốc Phi tập trung vào người lớn (≥18 tuổi). Kết quả Trong số 1008 nghiên cứu duy nhất được xác định, 54 nghiên cứu đáp ứng tiêu chuẩn đưa vào; 27 trong số đó là các thử nghiệm có đối chứng ngẫu nhiên. 50% các nghiên cứu đánh giá nhiều hơn một số liệu LS7 nhưng chỉ có hai nghiên cứu đánh giá cả bảy số liệu LS7. Không có nghiên cứu nào có tỷ lệ nam giới Mỹ gốc Phi cao. 40 nghiên cứu đã cải thiện ít nhất một số liệu LS7 tại điểm cuối của nghiên cứu. Nghiên cứu hình thành được sử dụng trong nhiều nghiên cứu để hướng dẫn thiết kế can thiệp. Các nghiên cứu có chất lượng khác nhau, nhưng nhìn chung được đánh giá là \"tốt\" khi sử dụng phương pháp tiếp cận đã sửa đổi đối với công cụ đánh giá chất lượng của Viện Y tế Quốc gia. Kết luận Không có đủ dữ liệu để đề xuất một can thiệp cụ thể dựa trên cộng đồng hoặc CBPR để cải thiện việc đạt được các số liệu LS7 trong số AA. Các nghiên cứu trong tương lai sử dụng phương pháp nghiêm ngặt với sự đa dạng giới tính gia tăng và sử dụng khuôn khổ LS7 của AHA là cần thiết để thiết lập một chương trình được xác nhận để cải thiện LS7 trong số AA."} +{"idx": 1871004, "en": "BACKGROUND Unhealthy food vending can expose children to malnutrition and other diet related challenges such as obesity. This study sought to describe types and sources of food in basic schools in urban Accra, and to describe food purchases by pupils. METHODS This was a cross-sectional study of five basic schools (3 public; 2 private) and 644 pupils in the Ga-East Municipality in Ghana. Check-lists were used to document available sources of foods during school hours. Pupils were intercepted after making purchases during breaktime and the type, cost and sources of foods purchased documented. Energy content of foods were read from labels when available or estimated using the Ghana Food Composition database when unlabelled. Frequencies and crosstabs were used to compare food type by source and school type. RESULTS Foods were purchased from school canteen, school store, private stores, and 'table-top' vendors. Meals were most frequently purchased (38%) although single purchases were sweetened drinks, savoury snacks and confectioneries. About 53% of retailers located within the schools sold relatively healthier food options. Similar foods with comparable energy content were purchased within and outside of school. CONCLUSIONS Basic schools in urban Ghana provide ready access to energy dense food options, which are purchased by pupils both within and outside of school premises. Timely interventions inclusive of school food policies can encourage healthier diets among pupils.", "gemini": "Bối cảnh Bán thực phẩm không lành mạnh có thể khiến trẻ em tiếp xúc với tình trạng suy dinh dưỡng và các thách thức liên quan đến chế độ ăn uống khác như béo phì. Nghiên cứu này tìm cách mô tả các loại và nguồn thực phẩm ở các trường cơ sở tại đô thị Accra và mô tả việc học sinh mua thực phẩm. Phương pháp Đây là một nghiên cứu cắt ngang về năm trường cơ sở (3 trường công; 2 trường tư) và 644 học sinh ở thành phố Ga-East, Ghana. Danh sách kiểm tra được sử dụng để ghi lại các nguồn thực phẩm có sẵn trong giờ học. Học sinh được chặn lại sau khi mua hàng trong giờ giải lao và loại, chi phí và nguồn thực phẩm đã mua được ghi lại. Hàm lượng năng lượng của thực phẩm được đọc từ nhãn khi có hoặc ước tính bằng cách sử dụng cơ sở dữ liệu thành phần thực phẩm của Ghana khi không có nhãn. Tần suất và bảng chéo được sử dụng để so sánh loại thực phẩm theo nguồn và loại trường. Kết quả Thực phẩm được mua từ căng tin trường học, cửa hàng trường học, cửa hàng tư nhân và người bán \"bán hàng rong\". Bữa ăn được mua nhiều nhất (38%) mặc dù đồ uống có đường, đồ ăn nhẹ mặn và đồ ngọt là những món được mua riêng lẻ. Khoảng 53% các nhà bán lẻ nằm trong khuôn viên trường bán các lựa chọn thực phẩm tương đối lành mạnh hơn. Các loại thực phẩm tương tự với hàm lượng năng lượng tương đương được mua trong và ngoài trường học. Kết luận Các trường cơ sở ở đô thị Ghana cung cấp sẵn các lựa chọn thực phẩm giàu năng lượng, được học sinh mua cả trong và ngoài khuôn viên trường. Các can thiệp kịp thời bao gồm các chính sách về thực phẩm của trường có thể khuyến khích chế độ ăn uống lành mạnh hơn cho học sinh."} +{"idx": 1871005, "en": "BACKGROUND Giving patients anti-osteoporotic agents peri-operatively is a well-accepted strategy to increase fusion rate and prevent complications. The purpose of this study was to investigate effectiveness of teriparatide and bisphosphonate on fusion surgery of thoracic and lumbar spine. METHODS We searched EMBASE and PubMed for randomized clinical trials (RCTs) and prospective comparative studies using teriparatide or bisphosphonate in peri-operative spinal fusion surgery. Our synthesized data of fusion rate, Oswestry disability index (ODI), and adverse event in contrast-based network meta-analysis. Pooled results were presented in risk ratio (RR) or mean difference (MD) with 95% confidence interval (CI). RESULTS Our search hit eight RCTs and three prospective studies with 676 patients receiving spinal surgery. Pooled result showed that teriparatide+Denosumab leads to significantly higher fusion rate than placebo (RR, 2.84; 95% CI: 1.22 to 6.60) and bisphosphonate (RR, 2.59; 95% CI: 1.13 to 5.96). We did not observe significant finding among placebo, teriparatide, and bisphosphonate in the two network models. CONCLUSION This is the first network meta-analysis providing an overview of the use of teriparatide and bisphosphonate for spinal fusion surgery. Teriparatide treatments are worth to be consider for spinal fusion surgery.", "gemini": "BỐI CẢNH Việc sử dụng thuốc chống loãng xương cho bệnh nhân trong giai đoạn quanh phẫu thuật là một chiến lược được chấp nhận rộng rãi nhằm tăng tỷ lệ kết hợp và ngăn ngừa biến chứng. Mục đích của nghiên cứu này là điều tra hiệu quả của teriparatide và bisphosphonate đối với phẫu thuật kết hợp cột sống ngực và thắt lưng. PHƯƠNG PHÁP Chúng tôi đã tìm kiếm EMBASE và PubMed để tìm các thử nghiệm lâm sàng ngẫu nhiên (RCT) và các nghiên cứu so sánh có triển vọng sử dụng teriparatide hoặc bisphosphonate trong phẫu thuật kết hợp cột sống quanh phẫu thuật. Dữ liệu tổng hợp của chúng tôi về tỷ lệ kết hợp, chỉ số tàn tật Oswestry (ODI) và tác dụng phụ trong phân tích siêu mạng dựa trên đối chứng. Kết quả gộp được trình bày theo tỷ lệ rủi ro (RR) hoặc chênh lệch trung bình (MD) với khoảng tin cậy (CI) 95%. KẾT QUẢ Tìm kiếm của chúng tôi tìm thấy tám RCT và ba nghiên cứu có triển vọng với 676 bệnh nhân được phẫu thuật cột sống. Kết quả gộp cho thấy teriparatide + Denosumab dẫn đến tỷ lệ kết hợp cao hơn đáng kể so với giả dược (RR, 2,84; CI 95%: 1,22 đến 6,60) và bisphosphonate (RR, 2,59; CI 95%: 1,13 đến 5,96). Chúng tôi không quan sát thấy phát hiện có ý nghĩa giữa giả dược, teriparatide và bisphosphonate trong hai mô hình mạng. KẾT LUẬN Đây là phân tích siêu mạng đầu tiên cung cấp tổng quan về việc sử dụng teriparatide và bisphosphonate cho phẫu thuật kết hợp cột sống. Các phương pháp điều trị bằng teriparatide đáng được cân nhắc cho phẫu thuật kết hợp cột sống."} +{"idx": 1871006, "en": "Visceral leishmaniasis caused by the protozoan Leishmania infantum is a zoonosis. The domestic dog is the primary reservoir in urban areas. This study aimed to evaluate the frequency, active infection and load of L. infantum in the genital tract of male and female dogs seropositive for this parasite, as well as to identify histological genital alterations associated with this protozoan. We studied 45 male and 25 female L. infantum-seropositive noncastrated dogs from the same endemic area in Brazil. Tissue samples from the testis, epididymis, prostate, vulva, vagina, and uterus were examined by singleplex qPCR and parasitological tests (histopathology, immunohistochemistry, and parasitological culture). The latter were performed for the detection of active infection (parasites able to multiply and to induce lesions). Forty-four (98%) males and 25 (100%) females were positive for L. infantum in the genital tract (epididymis: 98%; vulva: 92%; vagina: 92%; testis: 91%; uterus: 84%; prostate: 66%). Active infection in the genital tract was confirmed in 69% of males and 64% of females (32% in the uterus). Parasite loads were similar in the testis, vulva, epididymis and vagina and lower in the prostate. Only the parasite load in the vagina was significantly associated with the number of clinical signs. Granulomatous inflammation predominated in all organs, except for the prostate. Only in the testis and epididymis was the inflammatory infiltrate significantly more intense among dogs with a higher parasite load in these organs. The high frequency, detection of active infection and similarity of L. infantum loads in the genital tract of infected males and females suggest the potential of venereal transmission of this parasite by both sexes and of vertical transmission by females in the area studied. Additionally, vertical transmission may be frequent since active L. infantum infection was a common observation in the uterus.", "gemini": "Leishmaniasis nội tạng do động vật nguyên sinh Leishmania infantum gây ra là một bệnh ở động vật. Chó nhà là ổ chứa chính ở các khu vực đô thị. Nghiên cứu này nhằm đánh giá tần suất, nhiễm trùng hoạt động và tải lượng L. infantum ở đường sinh dục của chó đực và chó cái huyết thanh dương tính với ký sinh trùng này, cũng như xác định các biến đổi mô học ở bộ phận sinh dục liên quan đến động vật nguyên sinh này. Chúng tôi đã nghiên cứu 45 con chó đực và 25 con chó cái huyết thanh dương tính với L. infantum không bị thiến từ cùng một khu vực lưu hành ở Brazil. Các mẫu mô từ tinh hoàn, mào tinh hoàn, tuyến tiền liệt, âm hộ, âm đạo và tử cung được kiểm tra bằng qPCR đơn và các xét nghiệm ký sinh trùng (mô bệnh học, miễn dịch mô học và nuôi cấy ký sinh trùng). Các xét nghiệm sau được thực hiện để phát hiện nhiễm trùng hoạt động (ký sinh trùng có khả năng sinh sôi và gây tổn thương). Bốn mươi bốn (98%) con đực và 25 (100%) con cái dương tính với L. infantum ở đường sinh dục (mào tinh hoàn: 98%; âm hộ: 92%; âm đạo: 92%; tinh hoàn: 91%; tử cung: 84%; tuyến tiền liệt: 66%). Nhiễm trùng hoạt động ở đường sinh dục đã được xác nhận ở 69% nam và 64% nữ (32% ở tử cung). Tải lượng ký sinh trùng tương tự nhau ở tinh hoàn, âm hộ, mào tinh hoàn và âm đạo và thấp hơn ở tuyến tiền liệt. Chỉ có tải lượng ký sinh trùng ở âm đạo có liên quan đáng kể đến số lượng các dấu hiệu lâm sàng. Viêm hạt chiếm ưu thế ở tất cả các cơ quan, ngoại trừ tuyến tiền liệt. Chỉ ở tinh hoàn và mào tinh hoàn, thâm nhiễm viêm mới mạnh hơn đáng kể ở những con chó có tải lượng ký sinh trùng cao hơn ở các cơ quan này. Tần suất cao, phát hiện nhiễm trùng hoạt động và sự tương đồng về tải lượng L. infantum ở đường sinh dục của chó đực và chó cái bị nhiễm cho thấy khả năng lây truyền qua đường tình dục của ký sinh trùng này ở cả hai giới và khả năng lây truyền theo chiều dọc của chó cái trong khu vực nghiên cứu. Ngoài ra, lây truyền theo chiều dọc có thể thường xuyên vì nhiễm trùng L. infantum hoạt động là một quan sát phổ biến ở tử cung."} +{"idx": 1871007, "en": "AIMS To investigate the effectiveness of a novel jaw-opening-force measuring device as a screening tool to aid in the diagnosis of temporomandibular disorders (TMD). METHODS Symptomatic TMD patients (n = 58) and control TMD-free participants (n = 56) were screened by an oral medicine specialist according to the Diagnostic Criteria for TMD (DC/TMD). TMD patients were divided into three subcategories based on TMD symptoms (myofascial pain, disc displacement, and both combined). Jaw-opening forces were measured in both groups with an adjustable head device connected to a 1,000-N-load cell. Seven attempts were recorded at 10-second intervals by a data-capturing system. The geometric mean force values were obtained after discarding the first and last attempts. RESULTS TMD-free participants had greater jaw-opening forces than TMD patients both without and with adjustments for age, sex, height, and weight (both P < .001). The geometric mean ± standard deviation values for TMD patients were 18.5 ± 1.62 N and 47.7 ± 1.53 N for TMD-free participants. Differences in jaw-opening forces among the three TMD subcategories were not statistically significant; however, patients with disc displacement (23.7 ± 1.46 N) had greater jaw forces than patients with myofascial pain (17.0 ± 1.74 N) and both myofascial pain and disc displacement (17.0 ± 1.56 N). CONCLUSION This study demonstrated that differences in jaw-opening forces could be used as a diagnostic tool for TMD. Future studies should explore the potential of this device to measure improvement in jaw-opening forces following TMD treatment.", "gemini": "MỤC TIÊU Nghiên cứu hiệu quả của một thiết bị đo lực mở hàm mới như một công cụ sàng lọc để hỗ trợ chẩn đoán rối loạn khớp thái dương hàm (TMD). PHƯƠNG PHÁP Bệnh nhân TMD có triệu chứng (n = 58) và những người tham gia không bị TMD (n = 56) được một chuyên gia y học răng miệng sàng lọc theo Tiêu chuẩn chẩn đoán TMD (DC/TMD). Bệnh nhân TMD được chia thành ba phân nhóm dựa trên các triệu chứng TMD (đau cơ, lệch đĩa và cả hai kết hợp). Lực mở hàm được đo ở cả hai nhóm bằng một thiết bị đầu có thể điều chỉnh được kết nối với một tế bào tải 1.000 N. Bảy lần cố gắng được ghi lại ở các khoảng thời gian 10 giây bằng một hệ thống thu thập dữ liệu. Các giá trị lực trung bình hình học được thu được sau khi loại bỏ lần thử đầu tiên và cuối cùng. KẾT QUẢ Những người không bị TMD có lực mở hàm lớn hơn những bệnh nhân TMD cả khi có và không điều chỉnh theo tuổi, giới tính, chiều cao và cân nặng (cả hai đều có P < 0,001). Giá trị trung bình hình học ± độ lệch chuẩn đối với bệnh nhân TMD là 18,5 ± 1,62 N và 47,7 ± 1,53 N đối với những người không bị TMD. Sự khác biệt về lực mở hàm giữa ba phân nhóm TMD không có ý nghĩa thống kê; tuy nhiên, những bệnh nhân bị lệch đĩa (23,7 ± 1,46 N) có lực hàm lớn hơn những bệnh nhân bị đau cơ (17,0 ± 1,74 N) và cả đau cơ và lệch đĩa (17,0 ± 1,56 N). KẾT LUẬN Nghiên cứu này chứng minh rằng sự khác biệt về lực mở hàm có thể được sử dụng như một công cụ chẩn đoán TMD. Các nghiên cứu trong tương lai nên khám phá tiềm năng của thiết bị này trong việc đo lường sự cải thiện lực mở hàm sau khi điều trị TMD."} +{"idx": 1871008, "en": "Within the orofacial pain discipline, the most common group of afflictions is temporomandibular disorders (TMD). The pathologic and functional disorders included in this condition closely resemble those that are seen in the orthopedic medicine branch of the medical profession, so it would be expected that the same principles of orthopedic diagnosis and treatment are applied. Traditional orthopedic therapy relies on a \"Two Pathway\" approach involving conservative and/or surgical treatments. However, over the course of the 20th century, some members of the dental community have created another way of approaching these disorders- referred to in this paper as the \"Third Pathway\"-based on the assumption that signs and symptoms of TMD are due to a \"bad\" relationship between the mandible and skull, leading to a variety of irreversible occlusal or surgical corrective treatments. Since no other human joint is discussed in these terms within the orthopedic medicine communities, it has become progressively clear that the Third Pathway is a unique and artificial conceptual creation of the dental profession. However, many clinical studies have utilized the medically oriented conservative/surgical Two-Pathway model to diagnose and treat TMD within a biopsychosocial model of pain. These studies have shown that TMD comprise another domain of orthopedic illness that requires a medically oriented approach for good outcomes while avoiding the irreversible aspects of the Third Pathway. This review presents historical and current evidence that the Third Pathway is an example of unorthodox medicine that leads to unnecessary overtreatment and further proposes that it is time to abandon this approach as we move forward in the TMD field.", "gemini": "Trong lĩnh vực đau vùng mặt, nhóm bệnh lý phổ biến nhất là rối loạn khớp thái dương hàm (TMD). Các rối loạn về bệnh lý và chức năng có trong tình trạng này rất giống với các rối loạn được thấy trong chuyên ngành y học chỉnh hình, vì vậy có thể mong đợi rằng các nguyên tắc chẩn đoán và điều trị chỉnh hình cũng được áp dụng. Liệu pháp chỉnh hình truyền thống dựa trên cách tiếp cận \"Hai con đường\" liên quan đến các phương pháp điều trị bảo tồn và/hoặc phẫu thuật. Tuy nhiên, trong suốt thế kỷ 20, một số thành viên của cộng đồng nha khoa đã tạo ra một cách tiếp cận khác đối với các rối loạn này - được gọi trong bài báo này là \"Con đường thứ ba\" - dựa trên giả định rằng các dấu hiệu và triệu chứng của TMD là do mối quan hệ \"xấu\" giữa xương hàm và hộp sọ, dẫn đến nhiều phương pháp điều trị chỉnh nha hoặc phẫu thuật không thể đảo ngược. Vì không có khớp nào khác của con người được thảo luận theo các thuật ngữ này trong cộng đồng y học chỉnh hình, nên ngày càng rõ ràng rằng Con đường thứ ba là một sáng tạo khái niệm độc đáo và nhân tạo của ngành nha khoa. Tuy nhiên, nhiều nghiên cứu lâm sàng đã sử dụng mô hình Hai con đường bảo tồn/phẫu thuật theo hướng y tế để chẩn đoán và điều trị TMD trong một mô hình đau sinh học xã hội. Các nghiên cứu này đã chỉ ra rằng TMD bao gồm một lĩnh vực khác của bệnh chỉnh hình, đòi hỏi một cách tiếp cận theo hướng y tế để có kết quả tốt trong khi tránh các khía cạnh không thể đảo ngược của Con đường thứ ba. Tổng quan này trình bày bằng chứng lịch sử và hiện tại cho thấy Con đường thứ ba là một ví dụ về y học không chính thống dẫn đến điều trị quá mức không cần thiết và hơn nữa đề xuất rằng đã đến lúc từ bỏ cách tiếp cận này khi chúng ta tiến lên trong lĩnh vực TMD."} +{"idx": 1871009, "en": "AIMS To assess the effect of geographic tongue (GT) on taste, salivary flow, and pain characteristics in burning mouth syndrome (BMS) to determine whether GT is a contributing factor to BMS and whether BMS and GT represent similar patient populations. METHODS A retrospective chart study was conducted. Patients with a diagnosis of BMS or BMS/GT were included. Data regarding smell testing, spatial taste-testing, salivary flow, oral pH, and subjective pain rating on a generalized labeled magnitude scale (gLMS) were collected. RESULTS No significant differences in age, gender, oral pH, smell, or pain were found between groups. Stimulated and unstimulated salivary flow were significantly lower in BMS/GT. Taste responses to all taste stimuli and to ethanol were significantly lower in BMS, with the exception of sour at the fungiform papillae. CONCLUSION BMS and BMS/GT present with similar clinical pain phenotype and demographics; however, taste was more intact in BMS/GT, suggesting that GT may be a contributing factor in the development of BMS through a mechanism that does not involve taste.", "gemini": "MỤC ĐÍCH Đánh giá tác động của lưỡi địa lý (GT) đối với vị giác, lưu lượng nước bọt và các đặc điểm đau trong hội chứng bỏng miệng (BMS) để xác định xem GT có phải là yếu tố góp phần gây ra BMS hay không và liệu BMS và GT có đại diện cho quần thể bệnh nhân tương tự hay không. PHƯƠNG PHÁP Một nghiên cứu biểu đồ hồi cứu đã được tiến hành. Những bệnh nhân được chẩn đoán mắc BMS hoặc BMS/GT đã được đưa vào nghiên cứu. Dữ liệu liên quan đến xét nghiệm khứu giác, xét nghiệm vị giác không gian, lưu lượng nước bọt, độ pH trong miệng và đánh giá mức độ đau chủ quan trên thang cường độ được dán nhãn tổng quát (gLMS) đã được thu thập. KẾT QUẢ Không có sự khác biệt đáng kể về tuổi tác, giới tính, độ pH trong miệng, khứu giác hoặc đau giữa các nhóm. Lưu lượng nước bọt kích thích và không kích thích thấp hơn đáng kể ở BMS/GT. Phản ứng vị giác với tất cả các kích thích vị giác và với ethanol thấp hơn đáng kể ở BMS, ngoại trừ vị chua ở gai lưỡi. KẾT LUẬN BMS và BMS/GT biểu hiện với kiểu hình đau lâm sàng và thông tin nhân khẩu học tương tự; tuy nhiên, vị giác vẫn còn nguyên vẹn hơn ở BMS/GT, cho thấy GT có thể là một yếu tố góp phần vào sự phát triển của BMS thông qua một cơ chế không liên quan đến vị giác."} +{"idx": 1871010, "en": "AIMS To evaluate the available literature on structural and functional brain abnormalities in trigeminal neuralgia (TN) using several brain magnetic resonance imaging (MRI) techniques to further understand the central mechanisms of TN. METHODS PubMed and Web of Science databases and the reference lists of identified studies were searched to identify potentially eligible studies through January 2019. Eligible articles were assessed for risk of bias and reviewed by two independent researchers. RESULTS A total of 17 articles meeting the inclusion criteria were included in this study. The methodologic quality of the included studies was moderate. A total of 10 studies evaluated structural gray matter (GM) changes, and there was reasonable evidence that the GM of some specific brain regions changed in TN patients. In addition, there was a significant change in the root entry zone of the trigeminal nerve and in several regions of white matter. Functional changes in resting state were assessed in 9 studies. TN patients showed increased activation of resting state, and this activation was reduced in specific brain regions. There were several studies that focused on the correlation between functional parameters or strength of functional connectivity and clinical features (eg, visual analog score and pain duration), but each study focused on different brain areas or different functional connectivities within the brain. CONCLUSION There is moderate evidence that TN patients show structural brain differences in specific cortical and subcortical regions. In addition, TN patients show changes in pain-related functional connections in the resting state. Future research should focus on longitudinal designs and integration of different brain-imaging techniques.", "gemini": "MỤC ĐÍCH Đánh giá các tài liệu hiện có về các bất thường về cấu trúc và chức năng của não trong đau dây thần kinh sinh ba (TN) bằng cách sử dụng một số kỹ thuật chụp cộng hưởng từ não (MRI) để hiểu rõ hơn về cơ chế trung tâm của TN. PHƯƠNG PHÁP Các cơ sở dữ liệu PubMed và Web of Science cùng danh sách tham chiếu của các nghiên cứu đã xác định được đã được tìm kiếm để xác định các nghiên cứu đủ điều kiện tiềm năng cho đến tháng 1 năm 2019. Các bài báo đủ điều kiện được đánh giá rủi ro thiên vị và được hai nhà nghiên cứu độc lập xem xét. KẾT QUẢ Tổng cộng có 17 bài báo đáp ứng tiêu chí đưa vào được đưa vào nghiên cứu này. Chất lượng phương pháp luận của các nghiên cứu được đưa vào là trung bình. Tổng cộng có 10 nghiên cứu đánh giá các thay đổi về chất xám (GM) về mặt cấu trúc và có bằng chứng hợp lý cho thấy GM của một số vùng não cụ thể đã thay đổi ở bệnh nhân TN. Ngoài ra, có sự thay đổi đáng kể ở vùng rễ vào của dây thần kinh sinh ba và ở một số vùng chất trắng. Những thay đổi về chức năng trong trạng thái nghỉ ngơi đã được đánh giá trong 9 nghiên cứu. Bệnh nhân TN cho thấy sự gia tăng hoạt động của trạng thái nghỉ ngơi và sự gia tăng hoạt động này giảm ở các vùng não cụ thể. Có một số nghiên cứu tập trung vào mối tương quan giữa các thông số chức năng hoặc cường độ kết nối chức năng và các đặc điểm lâm sàng (ví dụ: điểm tương tự thị giác và thời gian đau), nhưng mỗi nghiên cứu tập trung vào các vùng não khác nhau hoặc các kết nối chức năng khác nhau trong não. KẾT LUẬN Có bằng chứng ở mức độ trung bình cho thấy bệnh nhân TN có sự khác biệt về cấu trúc não ở các vùng vỏ não và dưới vỏ não cụ thể. Ngoài ra, bệnh nhân TN có những thay đổi về các kết nối chức năng liên quan đến đau ở trạng thái nghỉ ngơi. Nghiên cứu trong tương lai nên tập trung vào các thiết kế theo chiều dọc và tích hợp các kỹ thuật chụp ảnh não khác nhau."} +{"idx": 1871011, "en": "AIMS To investigate pain sensitivity in the masseter muscle and index finger in response to acute psychologic stress in healthy participants. METHODS Fifteen healthy women (23.7 ± 2.3 years) participated in two randomized sessions: in the experimental stress session, the Paced Auditory Serial Addition Task (PASAT) was used to induce acute stress, and in the control session, a control task was performed. Salivary cortisol, perceived stress levels, electrical and pressure pain thresholds (PTs), and pain tolerance levels (PTLs) were measured at baseline and after each task. Mixed-model analysis was used to test for significant interaction effects between time and session. RESULTS An interaction effect between time and session occurred for perceived stress levels (P < .001); perceived stress was significantly higher after the experimental task than after the control task (P < .01). No interaction effects occurred for salivary cortisol levels, electrical PTs, or pressure PTLs. Although significant interactions did occur for electrical PTL (P < .05) and pressure PT (P < .001), the simple effects test could not identify significant differences between sessions at any time point. CONCLUSION The PASAT evoked significant levels of perceived stress; however, pain sensitivity to mechanical or electrical stimuli was not significantly altered in response to the stress task, and the salivary cortisol levels were not altered in response to the PASAT. These results must be interpreted with caution, and more studies with larger study samples are needed to increase the clinical relevant understanding of the pain mechanisms and psychologic stress.", "gemini": "MỤC ĐÍCH Nghiên cứu độ nhạy đau ở cơ cắn và ngón trỏ để phản ứng với căng thẳng tâm lý cấp tính ở những người tham gia khỏe mạnh. PHƯƠNG PHÁP Mười lăm phụ nữ khỏe mạnh (23,7 ± 2,3 tuổi) đã tham gia vào hai buổi được phân ngẫu nhiên: trong buổi căng thẳng thử nghiệm, Nhiệm vụ cộng số nối tiếp theo nhịp độ (PASAT) được sử dụng để gây ra căng thẳng cấp tính, và trong buổi kiểm soát, một nhiệm vụ kiểm soát được thực hiện. Cortisol nước bọt, mức độ căng thẳng được cảm nhận, ngưỡng đau điện và áp lực (PT) và mức độ chịu đau (PTL) được đo ở mức cơ bản và sau mỗi nhiệm vụ. Phân tích mô hình hỗn hợp được sử dụng để kiểm tra các hiệu ứng tương tác đáng kể giữa thời gian và phiên. KẾT QUẢ Hiệu ứng tương tác giữa thời gian và phiên xảy ra đối với mức độ căng thẳng được cảm nhận (P < 0,001); căng thẳng được cảm nhận cao hơn đáng kể sau nhiệm vụ thử nghiệm so với sau nhiệm vụ kiểm soát (P < 0,01). Không có hiệu ứng tương tác nào xảy ra đối với mức độ cortisol nước bọt, PT điện hoặc PTL áp lực. Mặc dù các tương tác đáng kể đã xảy ra đối với PTL điện (P < 0,05) và PT áp lực (P < 0,001), nhưng thử nghiệm hiệu ứng đơn giản không thể xác định được sự khác biệt đáng kể giữa các phiên tại bất kỳ thời điểm nào. KẾT LUẬN PASAT gợi ra mức độ căng thẳng được cảm nhận đáng kể; tuy nhiên, độ nhạy đau đối với các kích thích cơ học hoặc điện không thay đổi đáng kể để đáp ứng với nhiệm vụ căng thẳng và mức độ cortisol nước bọt không thay đổi để đáp ứng với PASAT. Những kết quả này phải được hiểu một cách thận trọng và cần có thêm các nghiên cứu với mẫu nghiên cứu lớn hơn để tăng sự hiểu biết có liên quan về mặt lâm sàng đối với các cơ chế đau và căng thẳng tâm lý."} +{"idx": 1871012, "en": "AIMS This study investigated the extent and characteristics of mental health issues in contemporary dance students, as clear insight into these health problems is lacking. METHODS During one academic year, 134 dance and dance-teacher students were monitored on a monthly basis using the Performing artist and Athlete Health Monitor (PAHM). RESULTS In total, 130 students were included in the analyses, comprising 81 dance students and 49 dance-teacher students. The response rate of the monthly questionnaires was 79.7%. Overall, 96.9% of the students reported at least one physical/mental health problem. The incidence proportion (IP) of all reported mental health issues for one academic year was 44.6% (n=58). Furthermore, 29.2% of the students reported a mental health issue as their most severe health problem, of whom 39.4% indicated a substantial mental health issue (i.e., problems leading to moderate, severe, or complete reductions in training volume or performance). The monthly IP of mental health issues ranged from 2.9 to 8.9% and 0 to 5.3% for substantial mental health issues. The most reported types of mental health issues were general anxiety (20.0%), stress due to external factors (18.3%), and constant tiredness (16.7%). Dance-teacher students of all study years and second-year students from both educational programs indicated significantly more mental health issues, while sex showed no significant differences. CONCLUSIONS Contemporary dance students are at risk of mental health issues. Dance schools should pay special attention to stress, anxiety, and (constant) tiredness of their students. More research is needed to gain insight into specific characteristics and risk factors of mental health issues in dance students.", "gemini": "MỤC ĐÍCH Nghiên cứu này đã điều tra mức độ và đặc điểm của các vấn đề sức khỏe tâm thần ở sinh viên múa đương đại, vì thiếu hiểu biết rõ ràng về các vấn đề sức khỏe này. PHƯƠNG PHÁP Trong một năm học, 134 sinh viên múa và giáo viên dạy múa được theo dõi hàng tháng bằng cách sử dụng Công cụ theo dõi sức khỏe của nghệ sĩ biểu diễn và vận động viên (PAHM). KẾT QUẢ Tổng cộng, 130 sinh viên được đưa vào phân tích, bao gồm 81 sinh viên múa và 49 sinh viên giáo viên dạy múa. Tỷ lệ phản hồi của các bảng câu hỏi hàng tháng là 79,7%. Nhìn chung, 96,9% sinh viên báo cáo ít nhất một vấn đề về sức khỏe thể chất/tâm thần. Tỷ lệ mắc (IP) của tất cả các vấn đề sức khỏe tâm thần được báo cáo trong một năm học là 44,6% (n = 58). Hơn nữa, 29,2% sinh viên báo cáo một vấn đề sức khỏe tâm thần là vấn đề sức khỏe nghiêm trọng nhất của họ, trong đó 39,4% chỉ ra một vấn đề sức khỏe tâm thần đáng kể (tức là các vấn đề dẫn đến giảm vừa phải, nghiêm trọng hoặc hoàn toàn về khối lượng tập luyện hoặc hiệu suất). IP hàng tháng của các vấn đề sức khỏe tâm thần dao động từ 2,9 đến 8,9% và từ 0 đến 5,3% đối với các vấn đề sức khỏe tâm thần đáng kể. Các loại vấn đề sức khỏe tâm thần được báo cáo nhiều nhất là lo lắng chung (20,0%), căng thẳng do các yếu tố bên ngoài (18,3%) và mệt mỏi liên tục (16,7%). Sinh viên giáo viên dạy múa của tất cả các năm học và sinh viên năm thứ hai của cả hai chương trình giáo dục cho biết có nhiều vấn đề sức khỏe tâm thần hơn đáng kể, trong khi giới tính không cho thấy sự khác biệt đáng kể. KẾT LUẬN Sinh viên múa đương đại có nguy cơ mắc các vấn đề sức khỏe tâm thần. Các trường dạy múa nên đặc biệt chú ý đến căng thẳng, lo lắng và mệt mỏi (liên tục) của học sinh. Cần có thêm nghiên cứu để hiểu sâu hơn về các đặc điểm cụ thể và các yếu tố nguy cơ của các vấn đề sức khỏe tâm thần ở sinh viên múa."} +{"idx": 1871013, "en": "AIMS The musician's hand represents a complex system, which requires important motor skills. Although several studies have already investigated rehabilitation outcomes and techniques in musicians after hand lesions, none have been addressed specifically to objectively quantifiable functional parameters. The purpose of our study was to study hand functionality in violinists in order to provide foundations on which to establish and develop more appropriate rehabilitation protocols. STUDY DESIGN An observational cross-sectional cohort study consisting of 34 subjects, including 23 students and 11 professional violinists who were either studying or working at a conservatory. Results were compared to a data set of a non-musician control group. METHODS Nine-hole peg test and hand test system (HTS) were used to perform the study. A hand-held dynamometer was used to measure hand grip and tripod pinch maximal isometric voluntary contraction of both hands. RESULTS Hand strength did not significantly differ on either side between professional and student violinists. A significant difference was seen when comparing violinists as a whole versus the non-musician control group. HTS highlighted significant differences in dexterity. CONCLUSION Violinists develop better overall motor performances of the left hand, and their performance is better than normal \"non-musician\" controls. Dexterity and precision of execution positively correlate to years of practice. We conclude that rehabilitation of a violinist's hand should be aimed at enhancing motor performance of the left hand and should be focused to maximize dexterity of both hands.", "gemini": "MỤC ĐÍCH Bàn tay của nhạc sĩ đại diện cho một hệ thống phức tạp, đòi hỏi các kỹ năng vận động quan trọng. Mặc dù một số nghiên cứu đã điều tra các kết quả và kỹ thuật phục hồi chức năng ở những người chơi nhạc sau tổn thương bàn tay, nhưng không có nghiên cứu nào được giải quyết cụ thể đến các thông số chức năng có thể định lượng được một cách khách quan. Mục đích của nghiên cứu của chúng tôi là nghiên cứu chức năng của bàn tay ở những người chơi vĩ cầm để cung cấp nền tảng để thiết lập và phát triển các phác đồ phục hồi chức năng phù hợp hơn. THIẾT KẾ NGHIÊN CỨU Một nghiên cứu nhóm cắt ngang quan sát bao gồm 34 đối tượng, trong đó có 23 sinh viên và 11 nghệ sĩ vĩ cầm chuyên nghiệp đang học hoặc làm việc tại một trường nhạc. Kết quả được so sánh với một tập dữ liệu của nhóm đối chứng không phải nhạc sĩ. PHƯƠNG PHÁP Kiểm tra chốt chín lỗ và hệ thống kiểm tra bàn tay (HTS) được sử dụng để thực hiện nghiên cứu. Một lực kế cầm tay được sử dụng để đo lực cầm tay và lực bóp ba chân co tối đa tự nguyện của cả hai tay. KẾT QUẢ Sức mạnh của bàn tay không khác biệt đáng kể ở cả hai bên giữa những người chơi vĩ cầm chuyên nghiệp và sinh viên. Một sự khác biệt đáng kể đã được nhìn thấy khi so sánh những người chơi vĩ cầm nói chung với nhóm đối chứng không phải nhạc sĩ. HTS làm nổi bật sự khác biệt đáng kể về sự khéo léo. KẾT LUẬN Những người chơi vĩ cầm phát triển hiệu suất vận động tổng thể tốt hơn của bàn tay trái và hiệu suất của họ tốt hơn so với những người kiểm soát \"không phải nhạc sĩ\" bình thường. Sự khéo léo và độ chính xác của thực hiện tương quan tích cực với số năm luyện tập. Chúng tôi kết luận rằng phục hồi chức năng bàn tay của người chơi vĩ cầm nên nhằm mục đích nâng cao hiệu suất vận động của bàn tay trái và nên tập trung vào việc tối đa hóa sự khéo léo của cả hai bàn tay."} +{"idx": 1871014, "en": "Exacerbation of nighttime sleep-related oromotor activity is often recognized as a relevant clinical entity commonly known as sleep bruxism (SB). Many pragmatic issues about SB diagnosis and management remain controversial. Therefore, within a critical review of the literature, this article proposes an operational clinical approach for SB diagnosis and management, with a focus on three comorbidities frequently occurring in relation to sleep: obstructive sleep apnea (OSA), gastroesophageal reflux disease (GERD), and insomnia. In the absence of any comorbidities, and if clinically justified, short-term medication and/or splints may be considered. If a comorbid condition is suspected, then the patient should be screened for OSA, GERD, and insomnia. For OSA screening, the Epworth Sleepiness Scale, STOP-Bang, and NoSAS questionnaires are available validated tools. For GERD screening, a positive patient report, whether associated or not with clinical signs and symptoms of heartburn and/or regurgitation, can be tested. For insomnia screening, report of difficulties initiating or maintaining sleep or of early morning awakening more than three times a week may be useful for diagnosis clarification. An adequate clinical approach for comorbid SB requires that both SB and the related comorbid condition be properly assessed and managed. Very often, improvement of SB with treatment of the associated condition will confirm the relationship and establish a more precise diagnosis (ie, secondary SB). Clinicians intending to manage SB should be able to identify these possible clinical interactions, and, if needed, perform an integrative multidimensional approach. Some approaches will benefit from a multidisciplinary approach for achieving therapeutic success.", "gemini": "Sự gia tăng các hoạt động oromotor liên quan đến giấc ngủ về đêm thường được công nhận là một thực thể lâm sàng có liên quan thường được gọi là nghiến răng khi ngủ (SB). Nhiều vấn đề thực dụng về chẩn đoán và quản lý SB vẫn còn gây tranh cãi. Do đó, trong phạm vi đánh giá phê phán các tài liệu, bài viết này đề xuất một phương pháp tiếp cận lâm sàng vận hành để chẩn đoán và quản lý SB, tập trung vào ba bệnh đi kèm thường xảy ra liên quan đến giấc ngủ: ngưng thở khi ngủ do tắc nghẽn (OSA), bệnh trào ngược dạ dày thực quản (GERD) và mất ngủ. Trong trường hợp không có bất kỳ bệnh đi kèm nào và nếu được chứng minh lâm sàng, có thể xem xét thuốc ngắn hạn và/hoặc máng nhai. Nếu nghi ngờ có tình trạng bệnh đi kèm, thì bệnh nhân nên được sàng lọc OSA, GERD và mất ngủ. Đối với sàng lọc OSA, thang điểm buồn ngủ Epworth, STOP-Bang và các bảng câu hỏi NoSAS là các công cụ được xác nhận có sẵn. Đối với sàng lọc GERD, có thể kiểm tra báo cáo của bệnh nhân có kết quả dương tính, cho dù có liên quan hay không với các dấu hiệu và triệu chứng lâm sàng của chứng ợ nóng và/hoặc trào ngược. Đối với sàng lọc mất ngủ, báo cáo về tình trạng khó bắt đầu hoặc duy trì giấc ngủ hoặc thức giấc vào sáng sớm nhiều hơn ba lần một tuần có thể hữu ích để làm rõ chẩn đoán. Một phương pháp tiếp cận lâm sàng đầy đủ cho SB đi kèm đòi hỏi phải đánh giá và quản lý đúng cả SB và tình trạng bệnh đi kèm liên quan. Rất thường xuyên, việc cải thiện SB khi điều trị tình trạng liên quan sẽ xác nhận mối quan hệ và thiết lập chẩn đoán chính xác hơn (tức là SB thứ phát). Các bác sĩ lâm sàng có ý định quản lý SB nên có khả năng xác định những tương tác lâm sàng có thể xảy ra này và nếu cần, hãy thực hiện một phương pháp tiếp cận đa chiều tích hợp. Một số phương pháp sẽ được hưởng lợi từ phương pháp tiếp cận đa ngành để đạt được thành công trong điều trị."} +{"idx": 1871015, "en": "AIMS Musicians spend numerous hours perfecting their skills and art, often leading to overuse injuries of the hand; of specific concern to musicians is carpal tunnel syndrome (CTS). This study evaluated the median nerve cross-sectional area and hand function of musicians and made comparisons to non-musicians. METHODS Seventy-six participants took part in the study, 38 music students and professors in the musician group and 38 participants in the control group. Participants completed patient-reported questionnaires to assess the level of pain and hand function. Ultrasound images were collected at the carpal tunnel inlet and outlet. The median nerve cross-sectional area and the depth of the carpal tunnel were measured on ultrasound images using software imbedded in the ultrasound unit. RESULTS Musicians showed higher levels of hand dysfunction and CTS symptoms than the non-musicians. The median nerve cross-sectional area was greater in musicians than in the non-musician group on both the right (mean difference 1.5 mm2, p=0.002) and left sides (mean difference 0.9 mm2, p=0.036). The depth of the carpal tunnel at the carpal tunnel inlet and outlet did not differ between the groups (p>0.05). CONCLUSION The current research identified between-group differences in median nerve cross-sectional area and the level of hand dysfunction. Understanding the interaction between the anatomy of the wrist and wrist and hand dysfunction will benefit clinicians when evaluating and treating musicians.", "gemini": "MỤC TIÊU Các nhạc sĩ dành nhiều giờ để hoàn thiện kỹ năng và nghệ thuật của mình, thường dẫn đến chấn thương do sử dụng quá mức ở bàn tay; hội chứng ống cổ tay (CTS) là mối quan tâm đặc biệt đối với các nhạc sĩ. Nghiên cứu này đã đánh giá diện tích mặt cắt ngang của dây thần kinh giữa và chức năng bàn tay của các nhạc sĩ và so sánh với những người không phải nhạc sĩ. PHƯƠNG PHÁP Bảy mươi sáu người tham gia đã tham gia nghiên cứu, 38 sinh viên âm nhạc và giáo sư trong nhóm nhạc sĩ và 38 người tham gia trong nhóm đối chứng. Những người tham gia đã hoàn thành các bảng câu hỏi do bệnh nhân tự báo cáo để đánh giá mức độ đau và chức năng của bàn tay. Hình ảnh siêu âm được thu thập tại lối vào và lối ra của ống cổ tay. Diện tích mặt cắt ngang của dây thần kinh giữa và độ sâu của ống cổ tay được đo trên hình ảnh siêu âm bằng phần mềm nhúng trong máy siêu âm. KẾT QUẢ Các nhạc sĩ cho thấy mức độ rối loạn chức năng bàn tay và các triệu chứng CTS cao hơn những người không phải nhạc sĩ. Diện tích mặt cắt ngang của dây thần kinh giữa lớn hơn ở nhóm nhạc sĩ so với nhóm không phải nhạc sĩ ở cả bên phải (chênh lệch trung bình 1,5 mm2, p = 0,002) và bên trái (chênh lệch trung bình 0,9 mm2, p = 0,036). Độ sâu của ống cổ tay ở lối vào và lối ra của ống cổ tay không khác nhau giữa các nhóm (p> 0,05). KẾT LUẬN Nghiên cứu hiện tại đã xác định sự khác biệt giữa các nhóm về diện tích mặt cắt ngang của dây thần kinh giữa và mức độ rối loạn chức năng bàn tay. Hiểu được sự tương tác giữa giải phẫu của cổ tay và rối loạn chức năng cổ tay và bàn tay sẽ có lợi cho các bác sĩ lâm sàng khi đánh giá và điều trị cho các nhạc sĩ."} +{"idx": 1871016, "en": "AIMS To examine if the existence of an association between self-reported awake oral behaviors and orofacial pain depends on the belief of patients that these behaviors are harmful to the jaw and to investigate if an additional variable (ie, somatic symptoms, depression, and/or anxiety) indirectly affects the association between the causal attribution belief and the report of awake oral behaviors. METHODS Prior to the first clinical visit, patients referred to a specialized clinic for complaints of orofacial pain and dysfunction completed a digital questionnaire. Data of 329 patients diagnosed with myalgia according to the Diagnostic Criteria for Temporomandibular Disorders (82.4% women; mean ± SD age = 41.9 ± 14.7 years) were analyzed. RESULTS Causal attribution belief moderated the association between awake oral behaviors and orofacial pain intensity. In addition, the relationship between causal attribution belief and self-reported oral behaviors was partially mediated by the presence of somatic symptoms (8%), depression (9%), and anxiety (16%). CONCLUSION Awake oral behaviors were positively associated with orofacial pain, but only under the condition of a strong belief of the patients in causal attribution of these behaviors to the jaw pain complaint. No such association was present in case of a low causal attribution belief. It appeared that, within this patient cohort, the relationship between causal attribution belief and self-reported oral behaviors was (in part) the result of shared psychologic risk factors.", "gemini": "MỤC TIÊU Để kiểm tra xem sự tồn tại của mối liên hệ giữa các hành vi thức tỉnh bằng miệng tự báo cáo và đau mặt hay không phụ thuộc vào niềm tin của bệnh nhân rằng những hành vi này có hại cho hàm và để điều tra xem một biến bổ sung (tức là các triệu chứng cơ thể, trầm cảm và/hoặc lo lắng) có ảnh hưởng gián tiếp đến mối liên hệ giữa niềm tin quy kết nguyên nhân và báo cáo về các hành vi thức tỉnh bằng miệng hay không. PHƯƠNG PHÁP Trước lần khám lâm sàng đầu tiên, những bệnh nhân được giới thiệu đến một phòng khám chuyên khoa vì các khiếu nại về đau và rối loạn chức năng mặt đã hoàn thành một bảng câu hỏi kỹ thuật số. Dữ liệu của 329 bệnh nhân được chẩn đoán mắc chứng đau cơ theo Tiêu chuẩn chẩn đoán rối loạn khớp thái dương hàm (82,4% là phụ nữ; độ tuổi trung bình ± SD = 41,9 ± 14,7) đã được phân tích. KẾT QUẢ Niềm tin quy kết nguyên nhân điều chỉnh mối liên hệ giữa các hành vi thức tỉnh bằng miệng và cường độ đau mặt. Ngoài ra, mối quan hệ giữa niềm tin quy kết nguyên nhân và các hành vi bằng miệng tự báo cáo một phần là do sự hiện diện của các triệu chứng cơ thể (8%), trầm cảm (9%) và lo lắng (16%). KẾT LUẬN Các hành vi thức tỉnh bằng miệng có liên quan tích cực đến đau mặt, nhưng chỉ trong điều kiện bệnh nhân tin tưởng mạnh mẽ vào việc quy kết nguyên nhân của những hành vi này cho các khiếu nại về đau hàm. Không có mối liên hệ nào như vậy trong trường hợp niềm tin quy kết nguyên nhân thấp. Có vẻ như trong nhóm bệnh nhân này, mối quan hệ giữa niềm tin quy kết nguyên nhân và các hành vi bằng miệng tự báo cáo (một phần) là kết quả của các yếu tố nguy cơ về tâm lý chung."} +{"idx": 1871017, "en": "OBJECTIVES To begin to establish normative data for shoulder range of motion (ROM) and strength in the circus acrobats and to compare these values based on age, sex, hand dominance, and acrobatic subgroup. METHODS Active (AROM) and passive (PROM) of the full shoulder complex and PROM of the isolated glenohumeral joint were measured in 193 circus acrobats using standardized techniques for anterior elevation (flexion), posterior elevation (extension), lateral elevation (abduction), and external and internal rotation. Shoulder strength was measured using a hand-held dynamometer in all planes of motion. Measurements were taken twice and averaged. Mixed ANOVA were performed. One-sample t-tests were used to compare with general population. RESULTS Several significant differences were noted between dominant and non-dominant sides, but not between the sex or age groups tested. Acrobats who did both aerial and ground acrobatics had significantly greater full shoulder complex flexion AROM than the aerial group, and AROM extension than the ground group. Circus acrobats had significantly greater AROM full shoulder complex extension, abduction, internal and external rotation, and shoulder strength than the general population. CONCLUSION Overall, results from this cross-sectional study revealed that circus acrobats had greater shoulder strength and ROM than the general population, which could affect the way these patients should be treated in a clinical setting. Age and sex seemed to have minimal effects, but there was clearly an effect of hand dominance. Acrobats who train both aerial and ground acrobatics may have greater ROM in certain planes than those who train in only one type of discipline.", "gemini": "MỤC TIÊU Bắt đầu thiết lập dữ liệu chuẩn cho phạm vi chuyển động (ROM) và sức mạnh vai ở các diễn viên xiếc nhào lộn và so sánh các giá trị này dựa trên tuổi, giới tính, sự thuận tay và nhóm nhào lộn. PHƯƠNG PHÁP Phạm vi chuyển động chủ động (AROM) và thụ động (PROM) của toàn bộ phức hợp vai và PROM của khớp vai riêng biệt đã được đo ở 193 diễn viên xiếc nhào lộn bằng các kỹ thuật chuẩn hóa cho động tác nâng trước (gập), nâng sau (duỗi), nâng ngang (xoay ngoài), và xoay trong và xoay ngoài. Sức mạnh vai được đo bằng máy đo lực cầm tay ở mọi mặt phẳng chuyển động. Các phép đo được thực hiện hai lần và tính trung bình. ANOVA hỗn hợp đã được thực hiện. Các bài kiểm tra t-test một mẫu đã được sử dụng để so sánh với dân số nói chung. KẾT QUẢ Một số khác biệt đáng kể đã được ghi nhận giữa các bên thuận và không thuận, nhưng không giữa các nhóm giới tính hoặc nhóm tuổi được kiểm tra. Các diễn viên xiếc nhào lộn thực hiện cả nhào lộn trên không và trên mặt đất có phạm vi chuyển động chủ động gập phức hợp vai toàn bộ lớn hơn đáng kể so với nhóm nhào lộn trên không và phạm vi chuyển động chủ động duỗi lớn hơn so với nhóm nhào lộn trên mặt đất. Các diễn viên xiếc nhào lộn có phạm vi chuyển động chủ động duỗi, xoay ngoài, xoay trong và sức mạnh vai phức hợp vai toàn bộ lớn hơn đáng kể so với dân số nói chung. KẾT LUẬN Nhìn chung, kết quả từ nghiên cứu cắt ngang này cho thấy các diễn viên xiếc nhào lộn có sức mạnh và phạm vi chuyển động vai lớn hơn so với dân số nói chung, điều này có thể ảnh hưởng đến cách điều trị những bệnh nhân này trong môi trường lâm sàng. Tuổi và giới tính dường như có ảnh hưởng tối thiểu, nhưng rõ ràng có sự ảnh hưởng của sự thuận tay. Các diễn viên xiếc nhào lộn tập luyện cả nhào lộn trên không và trên mặt đất có thể có phạm vi chuyển động lớn hơn ở một số mặt phẳng so với những người chỉ tập luyện một loại hình kỷ luật."} +{"idx": 1871018, "en": "AIMS To assess the effectiveness of a variety of physical treatments in the management of tension-type headache (TTH) in children. METHODS This review is reported in accordance with the PRISMA guidelines and was registered in the PROSPERO database (CRD42014015290). Randomized and nonrandomized controlled trials that examined the effects of all treatments with a physical component in the management of TTH in children and compared these treatments to a placebo intervention, no intervention, or a controlled comparison intervention were included. The Physiotherapy Evidence Database (PEDro) criteria for bias assessment and the Grades of Recommendation, Assessment, Development, and Evaluation (GRADE) Working Group criteria were used to assess the quality of the body of evidence. The outcome measures were pain, functioning, and quality of life. Only RCTs were included in the meta-analyses. RESULTS An initial search produced 10,464 published articles. Of these, 17 were relevant trials, including 1,815 participants. The overall GRADE rating of the included studies was moderate, and 11 of the 17 studies could be used in the meta-analyses. The effectiveness of physical treatments in terms of a reduction of pain of 50% or more showed a risk ratio (RR) of 2.37 (95% CI: 1.69 to 3.33). Relaxation training was the most evaluated intervention and proved to be significantly effective (RR: 3.00 [95% CI: 1.94 to 4.63]). In children having TTH combined with temporomandibular disorders, occlusal appliances were effective (RR: 2.58 [95% CI: 1.37 to 4.85]). CONCLUSION This review supports the use of physical treatments to reduce pain in children with TTH.", "gemini": "MỤC ĐÍCH Đánh giá hiệu quả của nhiều phương pháp điều trị vật lý khác nhau trong việc quản lý đau đầu do căng thẳng (TTH) ở trẻ em. PHƯƠNG PHÁP Đánh giá này được báo cáo theo hướng dẫn PRISMA và đã được đăng ký trong cơ sở dữ liệu PROSPERO (CRD42014015290). Các thử nghiệm có đối chứng ngẫu nhiên và không ngẫu nhiên kiểm tra tác dụng của tất cả các phương pháp điều trị có thành phần vật lý trong việc quản lý TTH ở trẻ em và so sánh các phương pháp điều trị này với can thiệp giả dược, không can thiệp hoặc can thiệp so sánh có kiểm soát đã được đưa vào. Tiêu chí Cơ sở dữ liệu bằng chứng về vật lý trị liệu (PEDro) để đánh giá độ thiên vị và tiêu chí của Nhóm làm việc về các cấp độ khuyến cáo, đánh giá, phát triển và đánh giá (GRADE) đã được sử dụng để đánh giá chất lượng của cơ sở dữ liệu bằng chứng. Các biện pháp kết quả là đau, chức năng và chất lượng cuộc sống. Chỉ có các RCT được đưa vào phân tích tổng hợp. KẾT QUẢ Tìm kiếm ban đầu cho ra 10.464 bài báo đã xuất bản. Trong số đó, có 17 thử nghiệm có liên quan, bao gồm 1.815 người tham gia. Xếp hạng GRADE chung của các nghiên cứu được đưa vào là trung bình và 11 trong số 17 nghiên cứu có thể được sử dụng trong phân tích tổng hợp. Hiệu quả của các phương pháp điều trị vật lý về việc giảm đau 50% trở lên cho thấy tỷ lệ nguy cơ (RR) là 2,37 (KTCB 95%: 1,69 đến 3,33). Huấn luyện thư giãn là can thiệp được đánh giá nhiều nhất và được chứng minh là có hiệu quả đáng kể (RR: 3,00 [KTCB 95%: 1,94 đến 4,63]). Ở trẻ em bị TTH kết hợp với rối loạn khớp thái dương hàm, dụng cụ chỉnh nha là hiệu quả (RR: 2,58 [KTCB 95%: 1,37 đến 4,85]). KẾT LUẬN Đánh giá này hỗ trợ việc sử dụng các phương pháp điều trị vật lý để giảm đau ở trẻ em bị TTH."} +{"idx": 1871019, "en": "AIMS Playing-related musculoskeletal disorders (PRMDs) are a significant health concern for percussionists. Although many of the known risk factors for PRMDs likely apply to all percussion subgroups (e.g., weekly practice hours, warm-ups/cool-downs, etc.), the rates and injury patterns in drummers (herein defined as 'percussionists who play the drum set') may differ due to differences in physical demands from those of other percussion subgroups. The goal of this study was to determine the drummer-specific rates and patterns of PRMDs. METHODS An electronic survey including questions on respondent demographics, history and patterns of PRMDs, and potential drummer-specific risk factors for reporting PRMDs was distributed via social media using a snowball sampling technique. The target population included individuals aged 18 years or older who exclusively played the drum set (minimum 5 hrs/wk). The rates of PRMDs were analyzed by body region (e.g., upper/lower limb, etc.) and by location within body regions (e.g., shoulder, knee joint, etc.). RESULTS The lifetime history of PRMDs in the study sample (n=831) was 68%, and 23% reported currently experiencing a PRMD. Most respondents reported multiple PRMDs (59%). The upper limb was the most commonly-affected body region (59%). The wrist joint (25%) and low back (24%) were the most commonly affected locations within body regions. CONCLUSIONS Drummers' reporting of multiple PRMDs is consistent with previous findings in percussionists, but differences in the lifetime histories and patterns of injury supports the notion that risk factors may differ between percussion subgroups. Analysis of survey responses pertaining to drummer-specific risk factors is currently underway.", "gemini": "MỤC ĐÍCH Các rối loạn cơ xương liên quan đến chơi nhạc (PRMD) là mối quan tâm đáng kể về sức khỏe đối với những người chơi bộ gõ. Mặc dù nhiều yếu tố nguy cơ đã biết đối với PRMD có thể áp dụng cho tất cả các nhóm nhạc cụ gõ (ví dụ: giờ luyện tập hàng tuần, khởi động/giãn cơ, v.v.), nhưng tỷ lệ và kiểu chấn thương ở những người đánh trống (ở đây được định nghĩa là 'những người chơi bộ gõ chơi trống') có thể khác nhau do nhu cầu thể chất khác với các nhóm nhạc cụ gõ khác. Mục tiêu của nghiên cứu này là xác định tỷ lệ và kiểu PRMD cụ thể của người đánh trống. PHƯƠNG PHÁP Một cuộc khảo sát điện tử bao gồm các câu hỏi về thông tin nhân khẩu của người trả lời, tiền sử và kiểu PRMD và các yếu tố nguy cơ cụ thể của người đánh trống khi báo cáo PRMD đã được phân phối qua phương tiện truyền thông xã hội bằng kỹ thuật lấy mẫu theo kiểu lăn cầu tuyết. Đối tượng mục tiêu bao gồm những người từ 18 tuổi trở lên chỉ chơi trống (tối thiểu 5 giờ/tuần). Tỷ lệ PRMD được phân tích theo vùng cơ thể (ví dụ: chi trên/chi dưới, v.v.) và theo vị trí trong vùng cơ thể (ví dụ: vai, khớp gối, v.v.). KẾT QUẢ Tiền sử PRMD trong mẫu nghiên cứu (n = 831) là 68% và 23% báo cáo hiện đang gặp phải PRMD. Hầu hết những người trả lời báo cáo nhiều PRMD (59%). Chi trên là vùng cơ thể bị ảnh hưởng phổ biến nhất (59%). Khớp cổ tay (25%) và thắt lưng (24%) là những vị trí bị ảnh hưởng phổ biến nhất trong vùng cơ thể. KẾT LUẬN Việc những người đánh trống báo cáo nhiều PRMD phù hợp với những phát hiện trước đây ở những người chơi bộ gõ, nhưng sự khác biệt về tiền sử và kiểu chấn thương hỗ trợ cho quan điểm rằng các yếu tố nguy cơ có thể khác nhau giữa các nhóm nhạc cụ gõ. Hiện đang tiến hành phân tích các phản hồi khảo sát liên quan đến các yếu tố nguy cơ cụ thể của người đánh trống."} +{"idx": 1871020, "en": "AIMS To examine the associations of self-reported presence of tinnitus with subtypes of temporomandibular disorders (TMD) as assessed by Axis I of the Diagnostic Criteria for TMD (DC/TMD) and with psychologic characteristics as assessed by Axis II. METHODS This retrospective controlled study included 108 consecutive TMD patients referred to the Tel Aviv University Orofacial Pain Clinic. Each patient received full Axis I and Axis II diagnoses according to the DC/TMD. The patients were asked about currently experiencing tinnitus. Pearson chi-square test and Fisher exact test were used to test the associations between categorical variables. Mann-Whitney test was used to assess differences in continuous variables between categories. A P value < .05 was considered statistically significant. RESULTS Thirty-three (30.6%) TMD patients reported experiencing tinnitus. There was a significantly higher prevalence of myofascial pain with referral (P = .008) and nonspecific physical symptoms (P = .014) among the TMD patients who reported tinnitus. In addition, those patients reported significantly longer pain duration compared to TMD patients without tinnitus (P = .039). CONCLUSION This study emphasizes the necessity of assessing both Axes I and II according to the DC/TMD in future studies and supports creating a standardized tinnitus screener tailored to TMD patients for future studies on tinnitus in TMD patients.", "gemini": "MỤC ĐÍCH Để kiểm tra mối liên quan giữa tình trạng ù tai tự báo cáo với các phân nhóm rối loạn khớp thái dương hàm (TMD) được đánh giá theo Trục I của Tiêu chuẩn chẩn đoán TMD (DC/TMD) và với các đặc điểm tâm lý được đánh giá theo Trục II. PHƯƠNG PHÁP Nghiên cứu có đối chứng hồi cứu này bao gồm 108 bệnh nhân TMD liên tiếp được giới thiệu đến Phòng khám Đau mặt và Miệng của Đại học Tel Aviv. Mỗi bệnh nhân được chẩn đoán đầy đủ theo Trục I và Trục II theo DC/TMD. Bệnh nhân được hỏi về tình trạng ù tai hiện tại. Thử nghiệm chi bình phương Pearson và thử nghiệm chính xác Fisher được sử dụng để kiểm tra mối liên quan giữa các biến phân loại. Thử nghiệm Mann-Whitney được sử dụng để đánh giá sự khác biệt về biến liên tục giữa các loại. Giá trị P < 0,05 được coi là có ý nghĩa thống kê. KẾT QUẢ Ba mươi ba (30,6%) bệnh nhân TMD báo cáo bị ù tai. Có tỷ lệ đau cơ hàm với triệu chứng đau lan (P = 0,008) và các triệu chứng về thể chất không đặc hiệu (P = 0,014) cao hơn đáng kể ở những bệnh nhân TMD báo cáo bị ù tai. Ngoài ra, những bệnh nhân này báo cáo thời gian đau kéo dài hơn đáng kể so với bệnh nhân TMD không bị ù tai (P = 0,039). KẾT LUẬN Nghiên cứu này nhấn mạnh sự cần thiết phải đánh giá cả Trục I và Trục II theo DC/TMD trong các nghiên cứu trong tương lai và hỗ trợ việc tạo ra một công cụ sàng lọc ù tai chuẩn hóa dành riêng cho bệnh nhân TMD cho các nghiên cứu trong tương lai về ù tai ở bệnh nhân TMD."} +{"idx": 1871021, "en": "Entrustable professional activities (EPAs) are a curriculum development and learner assessment tool that ensure a trainee is able to safely translate the skills they have learned during residency into unsupervised clinical practice. Although EPAs are used extensively across various health professions worldwide, dentistry is just beginning to call for their development at both the predoctoral and postgraduate levels. Given the complex, multifactorial nature of orofacial pain disorders and the need for an interdisciplinary approach to management, the specialty of orofacial pain is well suited to embracing EPAs to ensure program graduates are prepared for practice. Therefore, 10 EPAs have been developed in a combined effort from program directors from every CODA-accredited postgraduate orofacial pain residency program.", "gemini": "Các hoạt động chuyên môn có thể giao phó (EPA) là một công cụ phát triển chương trình giảng dạy và đánh giá người học đảm bảo rằng học viên có thể chuyển đổi an toàn các kỹ năng đã học trong thời gian nội trú thành thực hành lâm sàng không có sự giám sát. Mặc dù EPA được sử dụng rộng rãi trong nhiều ngành nghề y tế trên toàn thế giới, nhưng nha khoa mới chỉ bắt đầu kêu gọi phát triển EPA ở cả cấp độ tiền tiến sĩ và sau đại học. Với bản chất phức tạp, đa yếu tố của các rối loạn đau mặt và miệng và nhu cầu tiếp cận liên ngành để quản lý, chuyên khoa đau mặt và miệng rất phù hợp để áp dụng EPA nhằm đảm bảo rằng những người tốt nghiệp chương trình đã sẵn sàng hành nghề. Do đó, 10 EPA đã được phát triển trong nỗ lực chung của các giám đốc chương trình từ mọi chương trình nội trú về đau mặt và miệng sau đại học được CODA công nhận."} +{"idx": 1871022, "en": "AIMS Among musicians, string players have the highest prevalence for musculoskeletal overuse. Playing a violin or viola requires rapid, repetitive, and complicated movements of the hands and fingers. This cross-sectional study aimed to examine whether violin/viola, violin/cello, and violin/French horn players experience more intense musculoskeletal pain than other instrumentalists. METHODS The study sample consisted of 590 orchestra musicians (354 male, 236 female, mean age 36 yrs). Self-administered questionnaires were used to assess pain of the back, neck, shoulder, face, jaw, and upper extremity. Pain intensity during the last 7 days was measured by an 11-point numeric rating scale (NRS) with a score from 0 to 10, as well as was disadvantage at work and leisure. RESULTS Of the interviewed musicians, 20% presented playing-related musculoskeletal disorders at the time of the interview. Compared to other professional orchestra musicians, violin and viola players reported significantly more intense pain in the hand during the last week. Also, they had experienced more frequent neck pain ever and in 5 years than the others. During the past 30 days, violin and viola players had also perceived more harm in their upper limb joints. Violin/cello and violin/French horn players did not differ from the others. CONCLUSIONS Our study showed that musicians playing the violin or viola have more intense hand pain and more frequent neck pain than other musicians, but these seem to disturb their daily tasks only a little.", "gemini": "MỤC ĐÍCH Trong số các nhạc sĩ, người chơi đàn dây có tỷ lệ mắc các bệnh về cơ xương do sử dụng quá mức cao nhất. Chơi violin hoặc viola đòi hỏi các chuyển động nhanh, lặp đi lặp lại và phức tạp của bàn tay và ngón tay. Nghiên cứu cắt ngang này nhằm mục đích kiểm tra xem những người chơi violin/viola, violin/cello và violin/kèn cor có bị đau cơ xương dữ dội hơn những người chơi nhạc cụ khác hay không. PHƯƠNG PHÁP Mẫu nghiên cứu bao gồm 590 nhạc công dàn nhạc (354 nam, 236 nữ, độ tuổi trung bình 36). Các bảng câu hỏi tự quản được sử dụng để đánh giá cơn đau ở lưng, cổ, vai, mặt, hàm và chi trên. Cường độ đau trong 7 ngày qua được đo bằng thang đánh giá số từ 0 đến 10 (NRS) với điểm từ 0 đến 10, cũng như bất lợi trong công việc và giải trí. KẾT QUẢ Trong số những nhạc sĩ được phỏng vấn, 20% cho biết mắc các rối loạn cơ xương liên quan đến việc chơi nhạc tại thời điểm phỏng vấn. So với những nhạc công dàn nhạc chuyên nghiệp khác, những người chơi violin và viola báo cáo bị đau ở bàn tay dữ dội hơn đáng kể trong tuần qua. Ngoài ra, họ đã từng bị đau cổ nhiều hơn và trong 5 năm so với những người khác. Trong 30 ngày qua, những người chơi violin và viola cũng cảm thấy đau nhiều hơn ở các khớp chi trên. Những người chơi violin/cello và violin/kèn cor không khác biệt so với những người khác. KẾT LUẬN Nghiên cứu của chúng tôi cho thấy những nhạc sĩ chơi violin hoặc viola bị đau tay dữ dội hơn và đau cổ thường xuyên hơn những nhạc sĩ khác, nhưng những điều này dường như chỉ làm phiền một chút đến các công việc hàng ngày của họ."} +{"idx": 1871023, "en": "The von Willebrand factor ristocetin cofactor activity assay (VWF:RCo) is used for diagnosis of von Willebrand disease (VWD) because of its ability to evaluate VWF binding to platelets. VWF sequence variant p.D1472H is associated with lower VWF:RCo levels in the absence of associated bleeding symptoms, indicating the VWF:RCo may not be accurate for characterizing VWF function in individuals with this variant. Thus, this study aimed to determine the implications of the variant on VWF functioning in vivo. Mice were engineered with humanized wild-type (WT*) VWF A1/A2 and VWF with the p.D1472H (1472H) variant along with humanized platelet GPIbα and bred to homozygosity. VWF antigen and VWF binding to GPIbα were measured using enzyme-linked immunosorbent assay. Gel electrophoresis was used for VWF multimer analysis. Tail bleeding assays were performed at a 3-mm defined length. Normal VWF multimers were preserved in both WT* and 1472H mice. VWF expression was normal in the WT* and 1472H mice, and VWF binding to GPIbα did not statistically differ between the groups. Additionally, tail bleeding times were similar for WT* and 1472H mice. These results show the p.D1472H variant does not impair hemostasis in mice, and support the conclusion that p.D1472H is a normal variant in humans.", "gemini": "Xét nghiệm hoạt động của cofactor ristocetin của yếu tố von Willebrand (VWF: RCo) được sử dụng để chẩn đoán bệnh von Willebrand (VWD) vì xét nghiệm này có khả năng đánh giá sự liên kết của VWF với tiểu cầu. Biến thể trình tự VWF p.D1472H có liên quan đến mức VWF: RCo thấp hơn khi không có các triệu chứng chảy máu đi kèm, cho thấy VWF: RCo có thể không chính xác để mô tả chức năng VWF ở những cá nhân có biến thể này. Do đó, nghiên cứu này nhằm xác định những tác động của biến thể đối với chức năng VWF trong cơ thể sống. Chuột được thiết kế với VWF A1/A2 kiểu hoang dã (WT*) được nhân hóa và VWF có biến thể p.D1472H (1472H) cùng với GPIbα tiểu cầu được nhân hóa và lai để đồng hợp tử. Kháng nguyên VWF và sự liên kết của VWF với GPIbα được đo bằng xét nghiệm miễn dịch hấp phụ liên kết enzyme. Điện di gel được sử dụng để phân tích đa phân tử VWF. Các xét nghiệm chảy máu đuôi được thực hiện ở độ dài được xác định là 3 mm. Đa phân tử VWF bình thường được bảo tồn ở cả chuột WT* và 1472H. Biểu hiện VWF bình thường ở chuột WT* và 1472H, và sự liên kết của VWF với GPIbα không khác biệt đáng kể giữa các nhóm. Ngoài ra, thời gian chảy máu đuôi tương tự nhau ở chuột WT* và 1472H. Những kết quả này cho thấy biến thể p.D1472H không làm suy yếu quá trình cầm máu ở chuột và hỗ trợ cho kết luận rằng p.D1472H là một biến thể bình thường ở người."} +{"idx": 1871024, "en": "The polycomb group protein CBX2 is an important epigenetic reader involved in cell proliferation and differentiation. While CBX2 overexpression occurs in a wide range of human tumors, targeted deletion results in homeotic transformation, proliferative defects, and premature senescence. However, its cellular function(s) and whether it plays a role in maintenance of genome stability remain to be determined. Here, we demonstrate that loss of CBX2 in mouse fibroblasts induces abnormal large-scale chromatin structure and chromosome instability. Integrative transcriptome analysis and ATAC-seq revealed a significant dysregulation of transcripts involved in DNA repair, chromocenter formation, and tumorigenesis in addition to changes in chromatin accessibility of genes involved in lateral sclerosis, basal transcription factors, and folate metabolism. Notably, Cbx2-/- cells exhibit prominent decondensation of satellite DNA sequences at metaphase and increased sister chromatid recombination events leading to rampant chromosome instability. The presence of extensive centromere and telomere defects suggests a prominent role for CBX2 in heterochromatin homeostasis and the regulation of nuclear architecture.", "gemini": "Protein nhóm polycomb CBX2 là một đầu đọc biểu sinh quan trọng liên quan đến sự tăng sinh và biệt hóa tế bào. Trong khi sự biểu hiện quá mức CBX2 xảy ra ở nhiều loại khối u ở người thì việc xóa đích lại dẫn đến sự chuyển đổi homeotic, khiếm khuyết tăng sinh và lão hóa sớm. Tuy nhiên, chức năng tế bào của protein này và liệu nó có đóng vai trò trong việc duy trì sự ổn định của bộ gen hay không vẫn chưa được xác định. Ở đây, chúng tôi chứng minh rằng sự mất CBX2 ở nguyên bào sợi chuột gây ra cấu trúc chromatin bất thường quy mô lớn và sự bất ổn định của nhiễm sắc thể. Phân tích phiên mã tích hợp và ATAC-seq cho thấy sự mất điều hòa đáng kể của các bản sao liên quan đến sửa chữa DNA, sự hình thành trung tâm nhiễm sắc và ung thư ngoài những thay đổi về khả năng tiếp cận chromatin của các gen liên quan đến xơ cứng teo cơ bên, yếu tố phiên mã cơ bản và chuyển hóa folate. Đáng chú ý, các tế bào Cbx2-/- biểu hiện rõ sự ngưng tụ của các trình tự DNA vệ tinh ở kỳ giữa và tăng các sự kiện tái tổ hợp chromatid chị em dẫn đến sự bất ổn định nhiễm sắc thể tràn lan. Sự hiện diện của nhiều khiếm khuyết tâm động và tận cùng cho thấy vai trò nổi bật của CBX2 trong cân bằng dị nhiễm sắc thể và điều hòa cấu trúc nhân."} +{"idx": 1871025, "en": "Several statistical methods have been proposed for testing gene-environment (G-E) interactions under additive risk models using data from genome-wide association studies. However, these approaches have strong assumptions from underlying genetic models, such as dominant or recessive effects that are known to be less robust when the true genetic model is unknown. We aimed to develop a robust trend test employing a likelihood ratio test for detecting G-E interaction under an additive risk model, while incorporating the G-E independence assumption to increase power. We used a constrained likelihood to impose 2 sets of constraints for: 1) the linear trend effect of genotype and 2) the additive joint effects of gene and environment. To incorporate the G-E independence assumption, a retrospective likelihood was used versus a standard prospective likelihood. Numerical investigation suggests that the proposed tests are more powerful than tests assuming dominant, recessive, or general models under various parameter settings and under both likelihoods. Incorporation of the independence assumption enhances efficiency by 2.5-fold. We applied the proposed methods to examine the gene-smoking interaction for lung cancer and gene-apolipoprotein E $\\varepsilon$4 interaction for Alzheimer disease, which identified 2 interactions between apolipoprotein E $\\varepsilon$4 and loci membrane-spanning 4-domains subfamily A (MS4A) and bridging integrator 1 (BIN1) genes at genome-wide significance that were replicated using independent data.", "gemini": "Một số phương pháp thống kê đã được đề xuất để kiểm tra tương tác gen-môi trường (G-E) theo các mô hình rủi ro cộng gộp bằng cách sử dụng dữ liệu từ các nghiên cứu liên kết toàn bộ hệ gen. Tuy nhiên, các phương pháp tiếp cận này có những giả định mạnh mẽ từ các mô hình di truyền cơ bản, chẳng hạn như các tác động trội hoặc lặn được biết là ít mạnh mẽ hơn khi mô hình di truyền thực sự chưa được biết. Chúng tôi nhằm mục đích phát triển một bài kiểm tra xu hướng mạnh mẽ sử dụng bài kiểm tra tỷ lệ khả năng để phát hiện tương tác G-E theo mô hình rủi ro cộng gộp, đồng thời kết hợp giả định độc lập G-E để tăng sức mạnh. Chúng tôi đã sử dụng một khả năng bị ràng buộc để áp đặt 2 tập hợp ràng buộc cho: 1) hiệu ứng xu hướng tuyến tính của kiểu gen và 2) các hiệu ứng chung cộng gộp của gen và môi trường. Để kết hợp giả định độc lập G-E, một khả năng hồi cứu đã được sử dụng so với một khả năng tương lai tiêu chuẩn. Nghiên cứu số cho thấy các bài kiểm tra được đề xuất mạnh hơn các bài kiểm tra giả định các mô hình trội, lặn hoặc tổng quát theo nhiều cài đặt tham số khác nhau và theo cả hai khả năng. Việc kết hợp giả định độc lập làm tăng hiệu quả gấp 2,5 lần. Chúng tôi đã áp dụng các phương pháp được đề xuất để kiểm tra tương tác gen-hút thuốc đối với ung thư phổi và tương tác gen-apolipoprotein E $\\varepsilon$4 đối với bệnh Alzheimer, trong đó xác định được 2 tương tác giữa apolipoprotein E $\\varepsilon$4 và các gen phân họ miền xuyên màng 4 (MS4A) và gen liên kết cầu nối 1 (BIN1) ở mức độ có ý nghĩa toàn bộ hệ gen đã được sao chép bằng dữ liệu độc lập."} +{"idx": 1871026, "en": "Schmidingerella arcuata is an ecologically important tintinnid ciliate that has long served as a model species in plankton trophic ecology. We present a partial micronuclear genome and macronuclear transcriptome resource for S. arcuata, acquired using single-cell techniques, and we report on pilot analyses including functional annotation and genome architecture. Our analysis shows major fragmentation, elimination, and scrambling in the micronuclear genome of S. arcuata. This work introduces a new nonmodel genome resource for the study of ciliate ecology and genomic biology and provides a detailed functional counterpart to ecological research on S. arcuata.", "gemini": "Schmidingerella arcuata là một loài động vật nguyên sinh tintinnid quan trọng về mặt sinh thái, từ lâu đã được sử dụng làm loài mô hình trong sinh thái dinh dưỡng phù du. Chúng tôi trình bày một bộ gen vi nhân một phần và tài nguyên phiên mã vi nhân lớn cho S. arcuata, thu được bằng các kỹ thuật tế bào đơn, và chúng tôi báo cáo về các phân tích thí điểm bao gồm chú thích chức năng và kiến trúc bộ gen. Phân tích của chúng tôi cho thấy sự phân mảnh, loại bỏ và xáo trộn lớn trong bộ gen vi nhân của S. arcuata. Công trình này giới thiệu một nguồn tài nguyên bộ gen không phải mô hình mới để nghiên cứu về sinh thái động vật nguyên sinh và sinh học bộ gen, đồng thời cung cấp một đối tác chức năng chi tiết cho nghiên cứu sinh thái về S. arcuata."} +{"idx": 1871027, "en": "Current knowledge suggests that hypertension is in part mediated by immune mechanisms. Both interleukin (IL)-23 and IL-17 are up-regulated in several experimental hypertensive rodent models, as well as in hypertensive humans in observational studies. Recent preclinical studies have shown that either IL-23 or IL-17A treatment induce blood pressure elevation. However, the IL-23/IL-17 axis has not been a major therapeutic target in hypertension, unlike in other autoimmune diseases. In this review, we summarize current knowledge on the role of these cytokines in immune mechanisms contributing to hypertension, and discuss the potential of IL-23/IL-17-targeted therapy for treatment of hypertension.", "gemini": "Kiến thức hiện tại cho thấy tăng huyết áp một phần là do các cơ chế miễn dịch. Cả interleukin (IL) -23 và IL-17 đều được điều hòa tăng lên ở một số mô hình động vật gặm nhấm tăng huyết áp thực nghiệm, cũng như ở người tăng huyết áp trong các nghiên cứu quan sát. Các nghiên cứu tiền lâm sàng gần đây đã chỉ ra rằng điều trị bằng IL-23 hoặc IL-17A gây ra tình trạng tăng huyết áp. Tuy nhiên, trục IL-23 / IL-17 không phải là mục tiêu điều trị chính trong tăng huyết áp, không giống như ở các bệnh tự miễn khác. Trong bài đánh giá này, chúng tôi tóm tắt kiến thức hiện tại về vai trò của các cytokine này trong các cơ chế miễn dịch góp phần gây tăng huyết áp và thảo luận về tiềm năng của liệu pháp nhắm mục tiêu IL-23 / IL-17 để điều trị tăng huyết áp."} +{"idx": 1871028, "en": "The extent and duration of immunity following infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are critical outstanding questions about the epidemiology of this novel virus, and studies are needed to evaluate the effects of serostatus on reinfection. Understanding the potential sources of bias and methods for alleviating biases in these studies is important for informing their design and analysis. Confounding by individual-level risk factors in observational studies like these is relatively well appreciated. Here, we show how geographic structure and the underlying, natural dynamics of epidemics can also induce noncausal associations. We take the approach of simulating serological studies in the context of an uncontrolled or controlled epidemic, under different assumptions about whether prior infection does or does not protect an individual against subsequent infection, and using various designs and analytical approaches to analyze the simulated data. We find that in studies assessing whether seropositivity confers protection against future infection, comparing seropositive persons with seronegative persons with similar time-dependent patterns of exposure to infection by stratifying or matching on geographic location and time of enrollment is essential in order to prevent bias.", "gemini": "Mức độ và thời gian miễn dịch sau khi nhiễm vi-rút corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2) là những câu hỏi nổi bật quan trọng về dịch tễ học của loại vi-rút mới này và cần có các nghiên cứu để đánh giá tác động của tình trạng huyết thanh đối với tái nhiễm. Hiểu được các nguồn gây sai lệch tiềm ẩn và các phương pháp giảm thiểu sai lệch trong các nghiên cứu này là rất quan trọng để thông tin cho thiết kế và phân tích của họ. Sự nhiễu loạn bởi các yếu tố rủi ro ở cấp độ cá nhân trong các nghiên cứu quan sát như thế này được đánh giá tương đối tốt. Ở đây, chúng tôi chỉ ra cách cấu trúc địa lý và động lực tự nhiên cơ bản của dịch bệnh cũng có thể gây ra các mối liên hệ không có nguyên nhân. Chúng tôi thực hiện cách tiếp cận là mô phỏng các nghiên cứu huyết thanh học trong bối cảnh của một dịch bệnh không kiểm soát hoặc có kiểm soát, theo các giả định khác nhau về việc nhiễm trùng trước đó có bảo vệ một cá nhân khỏi nhiễm trùng tiếp theo hay không và sử dụng các thiết kế và phương pháp phân tích khác nhau để phân tích dữ liệu mô phỏng. Chúng tôi thấy rằng trong các nghiên cứu đánh giá xem huyết thanh dương tính có mang lại khả năng bảo vệ chống lại nhiễm trùng trong tương lai hay không, việc so sánh những người huyết thanh dương tính với những người huyết thanh âm tính có các mô hình tiếp xúc với nhiễm trùng theo thời gian phụ thuộc tương tự bằng cách phân tầng hoặc ghép đôi theo vị trí địa lý và thời gian đăng ký là rất cần thiết để ngăn ngừa sự thiên lệch."} +{"idx": 1871029, "en": "Health-care workers (HCWs) are at the frontline of response to coronavirus disease 2019 (COVID-19), being at a higher risk of acquiring the disease and, subsequently, exposing patients and others. Searches of 8 bibliographic databases were performed to systematically review the evidence on the prevalence, risk factors, clinical characteristics, and prognosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection among HCWs. A total of 97 studies (all published in 2020) met the inclusion criteria. The estimated prevalence of SARS-CoV-2 infection from HCWs' samples, using reverse transcription-polymerase chain reaction and the presence of antibodies, was 11% (95% confidence interval (CI): 7, 15) and 7% (95% CI: 4, 11), respectively. The most frequently affected personnel were nurses (48%, 95% CI: 41, 56), whereas most of the COVID-19-positive medical personnel were working in hospital nonemergency wards during screening (43%, 95% CI: 28, 59). Anosmia, fever, and myalgia were the only symptoms associated with HCW SARS-CoV-2 positivity. Among HCWs positive for COVID-19 by reverse transcription-polymerase chain reaction, 40% (95% CI: 17, 65) were asymptomatic at time of diagnosis. Finally, severe clinical complications developed in 5% (95% CI: 3, 8) of the COVID-19-positive HCWs, and 0.5% (95% CI: 0.02, 1.3) died. Health-care workers suffer a significant burden from COVID-19, with those working in hospital nonemergency wards and nurses being the most commonly infected personnel.", "gemini": "Nhân viên y tế (HCW) là tuyến đầu ứng phó với bệnh do virus corona 2019 (COVID-19), có nguy cơ mắc bệnh cao hơn và sau đó lây sang bệnh nhân và những người khác. Các tìm kiếm trên 8 cơ sở dữ liệu thư mục đã được thực hiện để xem xét một cách có hệ thống các bằng chứng về tỷ lệ mắc, các yếu tố nguy cơ, đặc điểm lâm sàng và tiên lượng của nhiễm virus corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2) ở HCW. Tổng cộng có 97 nghiên cứu (tất cả được công bố vào năm 2020) đáp ứng các tiêu chuẩn tuyển chọn. Tỷ lệ mắc ước tính của nhiễm SARS-CoV-2 từ các mẫu của HCW, sử dụng phản ứng phiên mã ngược-chuỗi polymerase và sự hiện diện của kháng thể, lần lượt là 11% (khoảng tin cậy 95% (CI): 7, 15) và 7% (95% CI: 4, 11). Những nhân viên bị ảnh hưởng nhiều nhất là y tá (48%, 95% CI: 41, 56), trong khi hầu hết các nhân viên y tế dương tính với COVID-19 đều làm việc tại các khoa không cấp cứu của bệnh viện trong quá trình sàng lọc (43%, 95% CI: 28, 59). Mất khứu giác, sốt và đau cơ là những triệu chứng duy nhất liên quan đến tình trạng dương tính với SARS-CoV-2 của HCW. Trong số các HCW dương tính với COVID-19 bằng phản ứng phiên mã ngược-chuỗi polymerase, 40% (95% CI: 17, 65) không có triệu chứng tại thời điểm chẩn đoán. Cuối cùng, các biến chứng lâm sàng nghiêm trọng đã phát triển ở 5% (95% CI: 3, 8) số HCW dương tính với COVID-19 và 0,5% (95% CI: 0,02, 1,3) tử vong. Nhân viên y tế phải chịu gánh nặng đáng kể do COVID-19, trong đó những người làm việc tại các khoa không cấp cứu của bệnh viện và y tá là những nhân viên thường bị nhiễm bệnh nhất."} +{"idx": 1871030, "en": "INTRODUCTION Military installations are at increased risk for the transmission of infectious disease. Personnel who live and train on military installations live and train near one another facilitating disease transmission. An understanding of historical sanitation and hygiene can inform modern practices. This is especially pertinent considering the continuing rise of variants of infectious diseases, such as the recent pandemic of the 2019 severe acute respiratory syndrome coronavirus 2. In this article, we review the rise and decline of infectious disease at the United States Military Academy (USMA) during the period spanning 1890 through 1910, and the public health interventions used to combat disease spread. MATERIALS AND METHODS Primary data regarding cadet illness were acquired from the historical archives of the USMA. These included annual reports, clinical admission records, casualty ledgers, and sanitation reports. Unpublished documents from the medical history of USMA provide periodic trends of health among cadets because of infectious disease. RESULTS Between 1890 and 1910, the USMA at West Point was confronted with cases of influenza, measles, mumps, scarlet fever, smallpox, typhus, and malaria. In response, a series of non-pharmaceutical interventions (NPIs) were instituted to curb the spread of infectious disease. These interventions most likely proved effective in suppressing the transmission of communicable diseases. The most common and arguably the most effective NPI was the physical separation of the sick from the well. CONCLUSIONS The USMA experience mirrored what was occurring in the larger U.S. Army in the early 20th century and may serve as a model for the application of NPIs in response to modern infectious diseases resulting from novel or unknown etiologies.", "gemini": "GIỚI THIỆU Các cơ sở quân sự có nguy cơ lây truyền bệnh truyền nhiễm cao hơn. Các nhân viên sống và huấn luyện tại các cơ sở quân sự sống và huấn luyện gần nhau tạo điều kiện thuận lợi cho việc lây truyền bệnh. Việc hiểu biết về vệ sinh và vệ sinh lịch sử có thể cung cấp thông tin cho các hoạt động hiện đại. Điều này đặc biệt liên quan khi xem xét sự gia tăng liên tục của các biến thể bệnh truyền nhiễm, chẳng hạn như đại dịch gần đây của virus corona gây hội chứng hô hấp cấp tính nặng 2019. Trong bài viết này, chúng tôi xem xét sự gia tăng và suy giảm của bệnh truyền nhiễm tại Học viện Quân sự Hoa Kỳ (USMA) trong giai đoạn từ năm 1890 đến năm 1910 và các biện pháp can thiệp y tế công cộng được sử dụng để chống lại sự lây lan của bệnh. VẬT LIỆU VÀ PHƯƠNG PHÁP Dữ liệu chính về bệnh tật của học viên được lấy từ kho lưu trữ lịch sử của USMA. Chúng bao gồm các báo cáo hàng năm, hồ sơ nhập viện lâm sàng, sổ cái thương vong và báo cáo vệ sinh. Các tài liệu chưa được công bố từ lịch sử y học của USMA cung cấp các xu hướng định kỳ về sức khỏe của học viên do bệnh truyền nhiễm. KẾT QUẢ Trong giai đoạn từ năm 1890 đến năm 1910, USMA tại West Point đã phải đối mặt với các trường hợp cúm, sởi, quai bị, sốt ban đỏ, đậu mùa, sốt phát ban và sốt rét. Để ứng phó, một loạt các biện pháp can thiệp phi dược phẩm (NPI) đã được đưa ra để ngăn chặn sự lây lan của bệnh truyền nhiễm. Những biện pháp can thiệp này rất có thể đã chứng minh hiệu quả trong việc ngăn chặn sự lây truyền các bệnh truyền nhiễm. Biện pháp can thiệp phi dược phẩm phổ biến nhất và có thể nói là hiệu quả nhất là cách ly vật lý những người bị bệnh với những người khỏe mạnh. KẾT LUẬN Kinh nghiệm của USMA phản ánh những gì đang diễn ra trong Quân đội Hoa Kỳ nói chung vào đầu thế kỷ 20 và có thể đóng vai trò là mô hình cho việc áp dụng các biện pháp can thiệp phi dược phẩm để ứng phó với các bệnh truyền nhiễm hiện đại do các nguyên nhân mới hoặc chưa biết gây ra."} +{"idx": 1871031, "en": "Insect wings are living, flexible structures composed of tubular veins and thin wing membrane. Wing veins can contain hemolymph (insect blood), tracheae, and nerves. Continuous flow of hemolymph within insect wings ensures that sensory hairs, structural elements such as resilin, and other living tissue within the wings remain functional. While it is well known that hemolymph circulates through insect wings, the extent of wing circulation (e.g., whether flow is present in every vein, and whether it is confined to the veins alone) is not well understood, especially for wings with complex wing venation. Over the last 100 years, scientists have developed experimental methods including microscopy, fluorescence, and thermography to observe flow in the wings. Recognizing and evaluating the importance of hemolymph movement in insect wings is critical in evaluating how the wings function both as flight appendages, as active sensors, and as thermoregulatory organs. In this review, we discuss the history of circulation in wings, past and present experimental techniques for measuring hemolymph, and broad implications for the field of hemodynamics in insect wings.", "gemini": "Cánh côn trùng là những cấu trúc linh hoạt, sống được tạo thành từ các tĩnh mạch hình ống và màng cánh mỏng. Các tĩnh mạch cánh có thể chứa huyết tương (máu côn trùng), khí quản và dây thần kinh. Dòng huyết tương liên tục bên trong cánh côn trùng đảm bảo rằng các sợi lông cảm giác, các thành phần cấu tạo như resilin và các mô sống khác bên trong cánh vẫn hoạt động. Mặc dù người ta biết rằng huyết tương lưu thông qua cánh côn trùng, nhưng mức độ lưu thông của cánh (ví dụ: dòng chảy có mặt ở mọi tĩnh mạch hay chỉ giới hạn ở riêng các tĩnh mạch) vẫn chưa được hiểu rõ, đặc biệt là đối với các cánh có hệ thống tĩnh mạch cánh phức tạp. Trong 100 năm qua, các nhà khoa học đã phát triển các phương pháp thực nghiệm bao gồm kính hiển vi, huỳnh quang và nhiệt đồ để quan sát dòng chảy trong cánh. Việc nhận biết và đánh giá tầm quan trọng của chuyển động huyết tương trong cánh côn trùng rất quan trọng trong việc đánh giá cách thức cánh hoạt động như các phần phụ của chuyến bay, như các cảm biến hoạt động và như các cơ quan điều nhiệt. Trong bài đánh giá này, chúng tôi thảo luận về lịch sử lưu thông trong cánh, các kỹ thuật thực nghiệm trong quá khứ và hiện tại để đo huyết tương và những ý nghĩa rộng lớn đối với lĩnh vực huyết động học trong cánh côn trùng."} +{"idx": 1871032, "en": "Recent declines in the health of the honey bee have startled researchers and lay people alike as honey bees are agriculture's most important pollinator. Honey bees are important pollinators of many major crops and add billions of dollars annually to the US economy through their services. One factor that may influence colony health is the microbial community. Indeed, the honey bee worker digestive tract harbors a characteristic community of bee-specific microbes, and the composition of this community is known to impact honey bee health. However, the honey bee is a superorganism, a colony of eusocial insects with overlapping generations where nestmates cooperate, building a hive, gathering and storing food, and raising brood. In contrast to what is known regarding the honey bee worker gut microbiome, less is known of the microbes associated with developing brood, with food stores, and with the rest of the built hive environment. More recently, the microbe Bombella apis was identified as associated with nectar, with developing larvae, and with honey bee queens. This bacterium is related to flower-associated microbes such as Saccharibacter floricola and other species in the genus Saccharibacter, and initial phylogenetic analyses placed it as sister to these environmental bacteria. Here, we used comparative genomics of multiple honey bee-associated strains and the nectar-associated Saccharibacter to identify genomic changes that may be associated with the ecological transition to honey bee association. We identified several genomic differences in the honey bee-associated strains, including a complete CRISPR/Cas system. Many of the changes we note here are predicted to confer upon Bombella the ability to survive in royal jelly and defend themselves against mobile elements, including phages. Our results are a first step toward identifying potential function of this microbe in the honey bee superorganism.", "gemini": "Gần đây, sức khỏe của loài ong mật đã suy giảm khiến các nhà nghiên cứu và người dân phải giật mình vì ong mật là loài thụ phấn quan trọng nhất trong nông nghiệp. Ong mật là loài thụ phấn quan trọng đối với nhiều loại cây trồng chính và hàng năm mang lại hàng tỷ đô la cho nền kinh tế Hoa Kỳ thông qua các dịch vụ của chúng. Một yếu tố có thể ảnh hưởng đến sức khỏe của đàn ong là cộng đồng vi khuẩn. Thật vậy, đường tiêu hóa của ong thợ chứa một cộng đồng đặc trưng gồm các vi khuẩn đặc hiệu của ong, và thành phần của cộng đồng này được biết là có tác động đến sức khỏe của ong mật. Tuy nhiên, ong mật là một siêu sinh vật, một đàn côn trùng xã hội với các thế hệ chồng chéo, trong đó các thành viên trong tổ hợp tác, xây dựng tổ ong, thu thập và dự trữ thức ăn, nuôi ấu trùng. Trái ngược với những gì đã biết về hệ vi sinh đường ruột của ong thợ, người ta biết ít hơn về các vi khuẩn liên quan đến việc phát triển ấu trùng, với kho dự trữ thức ăn và với phần còn lại của môi trường tổ ong được xây dựng. Gần đây, vi khuẩn Bombella apis đã được xác định là có liên quan đến mật hoa, với ấu trùng đang phát triển và với ong chúa. Loại vi khuẩn này có liên quan đến các vi khuẩn liên quan đến hoa như Saccharibacter floricola và các loài khác trong chi Saccharibacter, và các phân tích phát sinh loài ban đầu xếp nó là chị em với các vi khuẩn môi trường này. Ở đây, chúng tôi đã sử dụng bộ gen so sánh của nhiều chủng liên quan đến ong mật và Saccharibacter liên quan đến mật hoa để xác định những thay đổi về bộ gen có thể liên quan đến quá trình chuyển đổi sinh thái sang liên kết với ong mật. Chúng tôi đã xác định một số khác biệt về bộ gen ở các chủng liên quan đến ong mật, bao gồm cả hệ thống CRISPR/Cas hoàn chỉnh. Nhiều thay đổi mà chúng tôi ghi nhận ở đây được dự đoán sẽ mang lại cho Bombella khả năng sống sót trong sữa ong chúa và tự vệ chống lại các yếu tố di động, bao gồm cả thực khuẩn. Kết quả của chúng tôi là bước đầu tiên hướng tới việc xác định chức năng tiềm ẩn của loại vi khuẩn này trong siêu sinh vật ong mật."} +{"idx": 1871033, "en": "BACKGROUND Understanding the source of newly detected human papillomavirus (HPV) in middle-aged women is important to inform preventive strategies, such as screening and HPV vaccination. METHODS We conducted a prospective cohort study in Baltimore, Maryland. Women aged 35-60 years underwent HPV testing and completed health and sexual behavior questionnaires every 6 months over a 2-year period. New detection/loss of detection rates were calculated and adjusted hazard ratios were used to identify risk factors for new detection. RESULTS The new and loss of detection analyses included 731 women, and 104 positive for high-risk HPV. The rate of new high-risk HPV detection was 5.0 per 1000 woman-months. Reporting a new sex partner was associated with higher detection rates (adjusted hazard ratio, 8.1; 95% confidence interval, 3.5-18.6), but accounted only for 19.4% of all new detections. Among monogamous and sexually abstinent women, new detection was higher in women reporting ≥5 lifetime sexual partners than in those reporting <5 (adjusted hazard ratio, 2.2; 95% confidence interval, 1.2-4.2). CONCLUSION Although women remain at risk of HPV acquisition from new sex partners as they age, our results suggest that most new detections in middle-aged women reflect recurrence of previously acquired HPV.", "gemini": "BỐI CẢNH Hiểu được nguồn gốc của vi-rút papilloma ở người (HPV) mới được phát hiện ở phụ nữ trung niên là điều quan trọng để đưa ra các chiến lược phòng ngừa, chẳng hạn như sàng lọc và tiêm vắc-xin HPV. PHƯƠNG PHÁP Chúng tôi đã tiến hành một nghiên cứu theo dõi triển vọng ở Baltimore, Maryland. Phụ nữ từ 35-60 tuổi đã được xét nghiệm HPV và hoàn thành các bảng câu hỏi về sức khỏe và hành vi tình dục sau mỗi 6 tháng trong khoảng thời gian 2 năm. Tỷ lệ phát hiện/mất phát hiện mới được tính toán và tỷ lệ nguy cơ điều chỉnh được sử dụng để xác định các yếu tố nguy cơ đối với phát hiện mới. KẾT QUẢ Các phân tích phát hiện và mất phát hiện mới bao gồm 731 phụ nữ và 104 phụ nữ dương tính với HPV nguy cơ cao. Tỷ lệ phát hiện HPV nguy cơ cao mới là 5,0 trên 1000 tháng-phụ nữ. Việc báo cáo có bạn tình mới có liên quan đến tỷ lệ phát hiện cao hơn (tỷ lệ nguy cơ điều chỉnh, 8,1; khoảng tin cậy 95%, 3,5-18,6), nhưng chỉ chiếm 19,4% trong số tất cả các phát hiện mới. Trong số những phụ nữ chung thủy và kiêng quan hệ tình dục, phát hiện mới cao hơn ở những phụ nữ báo cáo có ≥5 bạn tình trong đời so với những phụ nữ báo cáo <5 (tỷ lệ nguy cơ điều chỉnh, 2,2; khoảng tin cậy 95%, 1,2-4,2). KẾT LUẬN Mặc dù phụ nữ vẫn có nguy cơ mắc HPV từ bạn tình mới khi họ già đi, nhưng kết quả của chúng tôi cho thấy rằng hầu hết các phát hiện mới ở phụ nữ trung niên phản ánh sự tái phát của HPV đã mắc trước đó."} +{"idx": 1871034, "en": "The understanding of how proteins evolve to perform novel functions has long been sought by biologists. In this regard, two homologous bacterial enzymes, PafA and Dop, pose an insightful case study, as both rely on similar mechanistic properties, yet catalyze different reactions. PafA conjugates a small protein tag to target proteins, whereas Dop removes the tag by hydrolysis. Given that both enzymes present a similar fold and high sequence similarity, we sought to identify the differences in the amino acid sequence and folding responsible for each distinct activity. We tackled this question using analysis of sequence-function relationships, and identified a set of uniquely conserved residues in each enzyme. Reciprocal mutagenesis of the hydrolase, Dop, completely abolished the native activity, at the same time yielding a catalytically active ligase. Based on the available Dop and PafA crystal structures, this change of activity required a conformational change of a critical loop at the vicinity of the active site. We identified the conserved positions essential for stabilization of the alternative loop conformation, and tracked alternative mutational pathways that lead to a change in activity. Remarkably, all these pathways were combined in the evolution of PafA and Dop, despite their redundant effect on activity. Overall, we identified the residues and structural elements in PafA and Dop responsible for their activity differences. This analysis delineated, in molecular terms, the changes required for the emergence of a new catalytic function from a preexisting one.", "gemini": "Các nhà sinh học từ lâu đã tìm cách hiểu về quá trình tiến hóa của protein để thực hiện các chức năng mới. Về vấn đề này, hai loại enzyme vi khuẩn đồng đẳng, PafA và Dop, đặt ra một trường hợp nghiên cứu sâu sắc, vì cả hai đều dựa trên các đặc tính cơ học tương tự, nhưng lại xúc tác các phản ứng khác nhau. PafA liên hợp một thẻ protein nhỏ với các protein đích, trong khi Dop loại bỏ thẻ bằng thủy phân. Do cả hai enzyme đều có cấu trúc tương tự và trình tự tương đồng cao, chúng tôi đã tìm cách xác định sự khác biệt về trình tự axit amin và sự gấp nếp chịu trách nhiệm cho từng hoạt động riêng biệt. Chúng tôi đã giải quyết câu hỏi này bằng cách sử dụng phân tích mối quan hệ giữa trình tự và chức năng, và xác định một tập hợp các phần tử được bảo tồn duy nhất trong mỗi enzyme. Đột biến qua lại của hydrolase, Dop, đã xóa sổ hoàn toàn hoạt động bản địa, đồng thời tạo ra một ligase có hoạt tính xúc tác. Dựa trên các cấu trúc tinh thể Dop và PafA hiện có, sự thay đổi hoạt động này đòi hỏi một sự thay đổi về cấu hình của một vòng lặp quan trọng ở gần vị trí hoạt động. Chúng tôi đã xác định các vị trí được bảo tồn cần thiết để ổn định cấu hình vòng lặp thay thế và theo dõi các con đường đột biến thay thế dẫn đến thay đổi hoạt động. Đáng chú ý, tất cả các con đường này đều được kết hợp trong quá trình tiến hóa của PafA và Dop, mặc dù chúng có tác dụng dư thừa đối với hoạt động. Nhìn chung, chúng tôi đã xác định các phần tử và thành phần cấu trúc trong PafA và Dop chịu trách nhiệm cho sự khác biệt hoạt động của chúng. Phân tích này đã mô tả, theo các thuật ngữ phân tử, những thay đổi cần thiết cho sự xuất hiện của một chức năng xúc tác mới từ một chức năng đã tồn tại trước đó."} +{"idx": 1871035, "en": "BACKGROUND Every clinical specimen is potentially infectious, but data regarding risk for contamination of the laboratory environment during routine testing are scarce. We assessed contamination during routine sample analysis in automated clinical chemistry and microbiology laboratories. METHODS A fluorescent marker was applied to specimen container exteriors to assess the impact of gross contamination. Nonpathogenic MS2 virus was added to remnant blood, urine, and ESwab matrices as a biomarker of cross-contamination. Samples were processed and analyzed using Roche Cobas 8100 and ISE, c502, e602, and c702 modules (blood) and BD Kiestra total laboratory automation (blood, urine, ESwabs) over 3 experiments. Fluorescence transfer to laboratory surfaces and personnel was visualized using ultraviolet light. Surfaces were swabbed and assessed for MS2 cross-contamination by RT-PCR. Adherence to standard precautions by laboratory staff was assessed by observation. RESULTS Fluorescence was observed on 49 of 165 (30%) laboratory surfaces and personnel and 21 of 93 (23%) total laboratory automation instruments. Fluorescence transferred most frequently to gloves (31/40), computer accessories (9/18), and specimen loading racks (12/12). None of 123 areas swabbed were positive for MS2. Improper personal protective equipment use occurred at a rate of 0.36 and 0.15 events per staff per hour in the chemistry and microbiology laboratories, respectively. Hand-washing compliance was observed for 61 of 132 (46%) staff members evaluated. CONCLUSIONS Analysis of grossly contaminated specimens on automated chemistry and microbiology equipment elicits a low likelihood of instrument contamination. However, handling contaminated specimen containers can result in contamination of environmental laboratory surfaces, representing a source of risk that is heightened by low adherence to appropriate personal protective equipment.", "gemini": "BỐI CẢNH Mỗi mẫu lâm sàng đều có khả năng gây nhiễm, nhưng dữ liệu về nguy cơ nhiễm bẩn môi trường phòng xét nghiệm trong quá trình xét nghiệm thường quy còn rất ít. Chúng tôi đã đánh giá tình trạng nhiễm bẩn trong quá trình phân tích mẫu thường quy tại các phòng xét nghiệm vi sinh và hóa học lâm sàng tự động. PHƯƠNG PHÁP Một chất đánh dấu huỳnh quang đã được sử dụng cho bên ngoài hộp đựng mẫu để đánh giá tác động của tình trạng nhiễm bẩn nghiêm trọng. Virus MS2 không gây bệnh đã được thêm vào các mẫu máu, nước tiểu và ESwab còn sót lại như một chất đánh dấu sinh học của tình trạng nhiễm chéo. Các mẫu được xử lý và phân tích bằng các mô-đun Roche Cobas 8100 và ISE, c502, e602 và c702 (máu) và hệ thống tự động hóa phòng xét nghiệm toàn phần BD Kiestra (máu, nước tiểu, ESwab) trong 3 thí nghiệm. Sự truyền huỳnh quang đến các bề mặt phòng xét nghiệm và nhân viên được quan sát bằng đèn cực tím. Các bề mặt được lấy tăm bông và đánh giá tình trạng nhiễm chéo MS2 bằng RT-PCR. Việc tuân thủ các biện pháp phòng ngừa tiêu chuẩn của nhân viên phòng xét nghiệm được đánh giá bằng cách quan sát. KẾT QUẢ Huỳnh quang được quan sát thấy trên 49 trong số 165 (30%) bề mặt phòng xét nghiệm và nhân viên và 21 trong số 93 (23%) tổng số dụng cụ tự động hóa phòng xét nghiệm. Huỳnh quang được truyền thường xuyên nhất đến găng tay (31/40), phụ kiện máy tính (9/18) và giá nạp mẫu (12/12). Không có khu vực nào trong số 123 khu vực được lấy tăm bông có kết quả dương tính với MS2. Việc sử dụng thiết bị bảo vệ cá nhân không đúng cách xảy ra với tỷ lệ 0,36 và 0,15 sự kiện trên mỗi nhân viên mỗi giờ tại các phòng xét nghiệm hóa học và vi sinh, tương ứng. Việc tuân thủ rửa tay đã được quan sát thấy ở 61 trong số 132 (46%) nhân viên được đánh giá. KẾT LUẬN Việc phân tích các mẫu bị nhiễm bẩn nghiêm trọng trên thiết bị hóa học và vi sinh tự động cho thấy khả năng nhiễm bẩn dụng cụ thấp. Tuy nhiên, việc xử lý các hộp đựng mẫu bị nhiễm bẩn có thể dẫn đến tình trạng nhiễm bẩn các bề mặt phòng xét nghiệm xung quanh, gây ra nguy cơ cao do việc tuân thủ các thiết bị bảo vệ cá nhân thích hợp thấp."} +{"idx": 1871036, "en": "BACKGROUND Plasma amino acid (PAA) profiles are used in routine clinical practice for the diagnosis and monitoring of inherited disorders of amino acid metabolism, organic acidemias, and urea cycle defects. Interpretation of PAA profiles is complex and requires substantial training and expertise to perform. Given previous demonstrations of the ability of machine learning (ML) algorithms to interpret complex clinical biochemistry data, we sought to determine if ML-derived classifiers could interpret PAA profiles with high predictive performance. METHODS We collected PAA profiling data routinely performed within a clinical biochemistry laboratory (2084 profiles) and developed decision support classifiers with several ML algorithms. We tested the generalization performance of each classifier using a nested cross-validation (CV) procedure and examined the effect of various subsampling, feature selection, and ensemble learning strategies. RESULTS The classifiers demonstrated excellent predictive performance, with the 3 ML algorithms tested producing comparable results. The best-performing ensemble binary classifier achieved a mean precision-recall (PR) AUC of 0.957 (95% CI 0.952, 0.962) and the best-performing ensemble multiclass classifier achieved a mean F4 score of 0.788 (0.773, 0.803). CONCLUSIONS This work builds upon previous demonstrations of the utility of ML-derived decision support tools in clinical biochemistry laboratories. Our findings suggest that, pending additional validation studies, such tools could potentially be used in routine clinical practice to streamline and aid the interpretation of PAA profiles. This would be particularly useful in laboratories with limited resources and large workloads. We provide the necessary code for other laboratories to develop their own decision support tools.", "gemini": "BỐI CẢNH Hồ sơ axit amin huyết tương (PAA) được sử dụng trong thực hành lâm sàng thường quy để chẩn đoán và theo dõi các rối loạn di truyền về chuyển hóa axit amin, axit hữu cơ máu và khiếm khuyết chu trình urê. Việc giải thích hồ sơ PAA rất phức tạp và đòi hỏi phải có đào tạo và chuyên môn đáng kể để thực hiện. Với những minh chứng trước đây về khả năng của các thuật toán học máy (ML) trong việc giải thích dữ liệu sinh hóa lâm sàng phức tạp, chúng tôi đã tìm cách xác định xem các bộ phân loại có nguồn gốc từ ML có thể giải thích hồ sơ PAA với hiệu suất dự đoán cao hay không. PHƯƠNG PHÁP Chúng tôi đã thu thập dữ liệu lập hồ sơ PAA thường được thực hiện trong phòng xét nghiệm sinh hóa lâm sàng (2084 hồ sơ) và phát triển các bộ phân loại hỗ trợ quyết định với một số thuật toán ML. Chúng tôi đã kiểm tra hiệu suất tổng quát của từng bộ phân loại bằng cách sử dụng quy trình kiểm định chéo lồng nhau (CV) và kiểm tra hiệu ứng của nhiều chiến lược lấy mẫu phụ, chọn tính năng và học tập tổng hợp. KẾT QUẢ Các bộ phân loại đã chứng minh hiệu suất dự đoán tuyệt vời, với 3 thuật toán ML được thử nghiệm cho kết quả tương đương. Bộ phân loại nhị phân tổng hợp có hiệu suất tốt nhất đạt được AUC độ chính xác-thu hồi (PR) trung bình là 0,957 (95% CI 0,952, 0,962) và bộ phân loại đa lớp tổng hợp có hiệu suất tốt nhất đạt được điểm F4 trung bình là 0,788 (0,773, 0,803). KẾT LUẬN Nghiên cứu này dựa trên những minh chứng trước đây về tiện ích của các công cụ hỗ trợ quyết định có nguồn gốc từ ML trong các phòng xét nghiệm sinh hóa lâm sàng. Những phát hiện của chúng tôi cho thấy rằng, khi chờ các nghiên cứu xác nhận bổ sung, các công cụ như vậy có khả năng được sử dụng trong thực hành lâm sàng thường quy để sắp xếp hợp lý và hỗ trợ việc giải thích hồ sơ PAA. Điều này sẽ đặc biệt hữu ích trong các phòng xét nghiệm có nguồn lực hạn chế và khối lượng công việc lớn. Chúng tôi cung cấp mã cần thiết để các phòng xét nghiệm khác phát triển các công cụ hỗ trợ quyết định của riêng họ."} +{"idx": 1871037, "en": "BACKGROUND For high-volume assays, optimizing throughput reduces test cost and turn-around time. One approach for liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays is sample multiplexing, wherein the analyte of interest is derivatized in different specimens with reagents of different molecular weight (differential mass tagging). Specimens can then be combined and simultaneously analyzed within a single injection to improve throughput. Here we developed and validated a quantitative, sample-multiplexed LC-MS/MS assay for serum total testosterone (TT) based on this approach. METHODS For the sample-multiplexed assay, calibrators, controls, and patient specimens were first extracted separately. After mass tagging with either methoxyamine or hydroxylamine, they were combined and injected into the LC-MS/MS system. To evaluate assay performance, we determined limit of quantification (LOQ), linearity, recovery, and imprecision. A method-comparison study was also performed, comparing the new assay with the standard LC-MS/MS assay in 1574 patient specimens. RESULTS The method was linear from 2.5 to 2000 ng/dL, with accuracies from 93% to 104% for both derivatives. An LOQ of 1.0 ng/dL was achieved. Intra-assay and total CVs across 4 quality control concentrations were less than 10%. The assay demonstrated good agreement (Deming regression, 1.03x + 6.07) with the standard LC-MS/MS assay for the patient specimens tested (TT, 3 to 4862 ng/dL). CONCLUSION Sample multiplexing by differential mass tagging of TT increases LC-MS/MS throughput 2-fold without compromising analytical accuracy and sensitivity.", "gemini": "BỐI CẢNH Đối với các xét nghiệm khối lượng lớn, việc tối ưu hóa thông lượng giúp giảm chi phí xét nghiệm và thời gian xử lý. Một phương pháp tiếp cận đối với các xét nghiệm sắc ký lỏng-phổ khối ghép nối (LC-MS/MS) là ghép mẫu, trong đó chất phân tích được quan tâm được dẫn xuất trong các mẫu khác nhau với các thuốc thử có trọng lượng phân tử khác nhau (đánh dấu khối lượng khác biệt). Sau đó, các mẫu có thể được kết hợp và phân tích đồng thời trong một lần tiêm duy nhất để cải thiện thông lượng. Tại đây, chúng tôi đã phát triển và xác nhận một xét nghiệm LC-MS/MS định lượng, ghép mẫu cho tổng testosterone huyết thanh (TT) dựa trên phương pháp tiếp cận này. PHƯƠNG PHÁP Đối với xét nghiệm ghép mẫu, các chất chuẩn, đối chứng và mẫu bệnh phẩm trước tiên được chiết xuất riêng biệt. Sau khi đánh dấu khối lượng bằng methoxyamine hoặc hydroxylamine, chúng được kết hợp và tiêm vào hệ thống LC-MS/MS. Để đánh giá hiệu suất xét nghiệm, chúng tôi đã xác định giới hạn định lượng (LOQ), tính tuyến tính, độ thu hồi và độ không chính xác. Một nghiên cứu so sánh phương pháp cũng được thực hiện, so sánh xét nghiệm mới với xét nghiệm LC-MS/MS tiêu chuẩn trong 1574 mẫu bệnh phẩm. KẾT QUẢ Phương pháp này có tính tuyến tính từ 2,5 đến 2000 ng/dL, với độ chính xác từ 93% đến 104% đối với cả hai dẫn xuất. Đã đạt được LOQ là 1,0 ng/dL. CV trong xét nghiệm và tổng thể trên 4 nồng độ kiểm soát chất lượng là dưới 10%. Xét nghiệm đã chứng minh sự phù hợp tốt (hồi quy Deming, 1,03x + 6,07) với xét nghiệm LC-MS/MS tiêu chuẩn đối với các mẫu bệnh phẩm được thử nghiệm (TT, từ 3 đến 4862 ng/dL). KẾT LUẬN Việc ghép mẫu bằng cách đánh dấu khối lượng khác biệt của TT làm tăng thông lượng LC-MS/MS gấp 2 lần mà không ảnh hưởng đến độ chính xác và độ nhạy phân tích."} +{"idx": 1871038, "en": "Liquid biopsy, particularly the analysis of circulating tumor DNA (ctDNA), has demonstrated considerable promise for numerous clinical intended uses. Successful validation and commercialization of novel ctDNA tests have the potential to improve the outcomes of patients with cancer. The goal of the Blood Profiling Atlas Consortium (BloodPAC) is to accelerate the development and validation of liquid biopsy assays that will be introduced into the clinic. To accomplish this goal, the BloodPAC conducts research in the following areas: Data Collection and Analysis within the BloodPAC Data Commons; Preanalytical Variables; Analytical Variables; Patient Context Variables; and Reimbursement. In this document, the BloodPAC's Analytical Variables Working Group (AV WG) attempts to define a set of generic analytical validation protocols tailored for ctDNA-based Next-Generation Sequencing (NGS) assays. Analytical validation of ctDNA assays poses several unique challenges that primarily arise from the fact that very few tumor-derived DNA molecules may be present in circulation relative to the amount of nontumor-derived cell-free DNA (cfDNA). These challenges include the exquisite level of sensitivity and specificity needed to detect ctDNA, the potential for false negatives in detecting these rare molecules, and the increased reliance on contrived samples to attain sufficient ctDNA for analytical validation. By addressing these unique challenges, the BloodPAC hopes to expedite sponsors' presubmission discussions with the Food and Drug Administration (FDA) with the protocols presented herein. By sharing best practices with the broader community, this work may also save the time and capacity of FDA reviewers through increased efficiency.", "gemini": "Sinh thiết lỏng, đặc biệt là phân tích DNA khối u lưu hành (ctDNA), đã chứng minh tiềm năng đáng kể cho nhiều mục đích sử dụng lâm sàng. Việc xác nhận và thương mại hóa thành công các xét nghiệm ctDNA mới có khả năng cải thiện kết quả của bệnh nhân ung thư. Mục tiêu của Liên minh Bản đồ Hồ sơ Máu (BloodPAC) là đẩy nhanh quá trình phát triển và xác nhận các xét nghiệm sinh thiết lỏng sẽ được đưa vào phòng khám. Để đạt được mục tiêu này, BloodPAC tiến hành nghiên cứu trong các lĩnh vực sau: Thu thập và phân tích dữ liệu trong BloodPAC Data Commons; Biến số tiền phân tích; Biến số phân tích; Biến số bối cảnh bệnh nhân; và Hoàn trả. Trong tài liệu này, Nhóm làm việc về biến số phân tích (AV WG) của BloodPAC cố gắng xác định một bộ các giao thức xác nhận phân tích chung được thiết kế riêng cho các xét nghiệm giải trình tự thế hệ tiếp theo (NGS) dựa trên ctDNA. Việc xác nhận phân tích các xét nghiệm ctDNA đặt ra một số thách thức độc đáo chủ yếu phát sinh từ thực tế là rất ít phân tử DNA có nguồn gốc từ khối u có thể có trong lưu thông so với lượng DNA không có tế bào khối u (cfDNA). Những thách thức này bao gồm mức độ nhạy và độ đặc hiệu tuyệt vời cần thiết để phát hiện ctDNA, khả năng xảy ra âm tính giả khi phát hiện các phân tử hiếm này và sự phụ thuộc ngày càng tăng vào các mẫu chế tạo để đạt được đủ ctDNA để xác nhận phân tích. Bằng cách giải quyết những thách thức độc đáo này, BloodPAC hy vọng sẽ đẩy nhanh các cuộc thảo luận trước khi nộp đơn của các nhà tài trợ với Cục Quản lý Thực phẩm và Dược phẩm (FDA) với các giao thức được trình bày tại đây. Bằng cách chia sẻ các phương pháp hay nhất với cộng đồng rộng lớn hơn, công việc này cũng có thể tiết kiệm thời gian và năng lực của những người đánh giá của FDA thông qua việc tăng hiệu quả."} +{"idx": 1871039, "en": "BACKGROUND Concerns exist regarding how the 99th percentile upper reference limit (URL) of cardiac troponin (cTn) is determined and whether it should be derived from normal healthy individuals. CONTENT The 99th percentile URL of cTn is an important criterion to standardize the diagnosis of myocardial infarction (MI) for clinical, research, and regulatory purposes. Statistical heterogeneity in its calculation exists but recommendations have been proposed. Some negativity has resulted from the fact that with some high-sensitivity (hs) cTn assays, a greater number of increases above the 99th percentile are observed when transitioning from a contemporary assay. Increases reflect acute or chronic myocardial injury and provide valuable diagnostic and prognostic information. The etiology of increases can sometimes be difficult to determine, making a specific treatment approach challenging. For those reasons, some advocate higher cutoff concentrations. This approach can contribute to missed diagnoses. Contrary to claims, neither clinical or laboratory guidelines have shifted away from the 99th percentile. To support the diagnosis of acute MI, the 99th percentile URL remains the best-established approach given the absence of cTn assay standardization. Importantly, risk stratification algorithms using hs-cTn assays predict the possibility of MI diagnoses established using the 99th percentile. SUMMARY The 99th percentile of cTn remains the best-established criterion for the diagnosis of acute MI. While not perfect, it is analytically and clinically evidence-based. Until there are robust data to suggest some other approach, staying with the 99th percentile, a threshold that has served the field well for the past 20 years, appears prudent.", "gemini": "BỐI CẢNH Có những lo ngại về cách xác định giới hạn tham chiếu trên ở phần trăm thứ 99 (URL) của troponin tim (cTn) và liệu nó có nên bắt nguồn từ những cá nhân khỏe mạnh bình thường hay không. NỘI DUNG URL phần trăm thứ 99 của cTn là một tiêu chí quan trọng để chuẩn hóa chẩn đoán nhồi máu cơ tim (MI) cho mục đích lâm sàng, nghiên cứu và quản lý. Tính không đồng nhất về mặt thống kê trong tính toán của nó tồn tại nhưng các khuyến nghị đã được đề xuất. Một số tiêu cực đã phát sinh từ thực tế là với một số xét nghiệm cTn độ nhạy cao (hs), số lượng tăng trên phần trăm thứ 99 được quan sát thấy nhiều hơn khi chuyển từ xét nghiệm đương đại. Sự gia tăng phản ánh tổn thương cơ tim cấp tính hoặc mãn tính và cung cấp thông tin chẩn đoán và tiên lượng có giá trị. Đôi khi rất khó xác định nguyên nhân gây ra sự gia tăng, khiến cho cách tiếp cận điều trị cụ thể trở nên khó khăn. Vì những lý do đó, một số người ủng hộ nồng độ ngắt cao hơn. Cách tiếp cận này có thể góp phần bỏ sót chẩn đoán. Trái với những tuyên bố, không có hướng dẫn lâm sàng hoặc phòng xét nghiệm nào chuyển hướng khỏi phần trăm thứ 99. Để hỗ trợ chẩn đoán MI cấp, URL phần trăm thứ 99 vẫn là cách tiếp cận được thiết lập tốt nhất do không có tiêu chuẩn hóa xét nghiệm cTn. Điều quan trọng là các thuật toán phân tầng rủi ro sử dụng các xét nghiệm hs-cTn dự đoán khả năng chẩn đoán MI được thiết lập bằng cách sử dụng phần trăm thứ 99. TÓM TẮT Phần trăm thứ 99 của cTn vẫn là tiêu chuẩn được thiết lập tốt nhất để chẩn đoán MI cấp. Mặc dù không hoàn hảo nhưng nó dựa trên bằng chứng về mặt phân tích và lâm sàng. Cho đến khi có dữ liệu đáng tin cậy để gợi ý một số cách tiếp cận khác, việc duy trì ở phần trăm thứ 99, một ngưỡng đã phục vụ tốt cho lĩnh vực này trong 20 năm qua, có vẻ là điều thận trọng."} +{"idx": 1871040, "en": "Correspondence between evolution and development has been discussed for more than two centuries. Recent work reveals that phylogeny-ontogeny correlations are indeed present in developmental transcriptomes of eukaryotic clades with complex multicellularity. Nevertheless, it has been largely ignored that the pervasive presence of phylogeny-ontogeny correlations is a hallmark of development in eukaryotes. This perspective opens a possibility to look for similar parallelisms in biological settings where developmental logic and multicellular complexity are more obscure. For instance, it has been increasingly recognized that multicellular behavior underlies biofilm formation in bacteria. However, it remains unclear whether bacterial biofilm growth shares some basic principles with development in complex eukaryotes. Here we show that the ontogeny of growing Bacillus subtilis biofilms recapitulates phylogeny at the expression level. Using time-resolved transcriptome and proteome profiles, we found that biofilm ontogeny correlates with the evolutionary measures, in a way that evolutionary younger and more diverged genes were increasingly expressed toward later timepoints of biofilm growth. Molecular and morphological signatures also revealed that biofilm growth is highly regulated and organized into discrete ontogenetic stages, analogous to those of eukaryotic embryos. Together, this suggests that biofilm formation in Bacillus is a bona fide developmental process comparable to organismal development in animals, plants, and fungi. Given that most cells on Earth reside in the form of biofilms and that biofilms represent the oldest known fossils, we anticipate that the widely adopted vision of the first life as a single-cell and free-living organism needs rethinking.", "gemini": "Sự tương ứng giữa tiến hóa và phát triển đã được thảo luận trong hơn hai thế kỷ. Các công trình gần đây cho thấy các tương quan giữa phả hệ và phôi thai thực sự có mặt trong các bản sao mã phát triển của các nhóm sinh vật nhân chuẩn có đa bào phức tạp. Tuy nhiên, người ta phần lớn bỏ qua rằng sự hiện diện phổ biến của các tương quan giữa phả hệ và phôi thai là một đặc điểm của sự phát triển ở sinh vật nhân chuẩn. Quan điểm này mở ra khả năng tìm kiếm những điểm tương đồng song song trong các bối cảnh sinh học mà logic phát triển và sự phức tạp đa bào ít rõ ràng hơn. Ví dụ, người ta ngày càng nhận ra rằng hành vi đa bào nằm dưới cơ sở hình thành màng sinh học ở vi khuẩn. Tuy nhiên, vẫn chưa rõ liệu sự phát triển của màng sinh học ở vi khuẩn có chia sẻ một số nguyên tắc cơ bản với sự phát triển ở sinh vật nhân chuẩn phức tạp hay không. Ở đây chúng tôi chỉ ra rằng quá trình phát triển của màng sinh học Bacillus subtilis đang phát triển tóm tắt lại phả hệ ở cấp độ biểu hiện. Sử dụng các cấu hình phiên mã và proteome được giải quyết theo thời gian, chúng tôi thấy rằng quá trình phát triển màng sinh học tương quan với các biện pháp tiến hóa, theo cách mà các gen trẻ hơn và phân kỳ hơn về mặt tiến hóa ngày càng được biểu hiện nhiều hơn theo các thời điểm sau của quá trình phát triển màng sinh học. Các đặc điểm phân tử và hình thái cũng cho thấy rằng sự phát triển của màng sinh học được điều chỉnh cao và được tổ chức thành các giai đoạn phát sinh riêng biệt, tương tự như các giai đoạn của phôi sinh vật nhân chuẩn. Cùng nhau, điều này cho thấy rằng sự hình thành màng sinh học ở Bacillus là một quá trình phát triển trung thực có thể so sánh với sự phát triển của cơ thể ở động vật, thực vật và nấm. Do hầu hết các tế bào trên Trái đất cư trú dưới dạng màng sinh học và màng sinh học đại diện cho các hóa thạch lâu đời nhất được biết đến, chúng tôi dự đoán rằng tầm nhìn được chấp nhận rộng rãi về sự sống đầu tiên là một tế bào đơn lẻ và sinh vật sống tự do cần được xem xét lại."} +{"idx": 1871041, "en": "BACKGROUND A novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) occurred in China in December 2019 and has spread globally. In this study, we aimed to describe the clinical characteristics and outcomes of hospitalized older adults with coronavirus disease 2019 (COVID-19) in Turkey. METHODS We retrospectively analyzed the clinical data of hospitalized patients aged ≥ 60 years with confirmed COVID-19 from March 11, 2020 to May 27, 2020 using nationwide health database. RESULTS In this nationwide cohort, a total of 16,942 hospitalized older adults with COVID-19 were enrolled, of whom 8,635 (51%) were women. Mean age was 71.2 ± 8.5 years, ranging from 60 to 113 years. Mortality rate before and after curfew was statistically different (32.2% vs. 17.9%; p < .001, respectively). Through multivariate analysis of the causes of death in older patients, we found that male gender, diabetes mellitus, heart failure, dementia, were all significantly associated with mortality in entire cohort. In addition to abovementioned risk factors, in patients aged between 60 and 79 years, hypertension, chronic kidney disease and cancer and in patients 80 years of age and older malnutrition were also associated with increased risk of mortality. CONCLUSIONS In addition to the results of previous studies with smaller sample size, our results confirmed the age-related relationship between specific comorbidities and COVID-19-related mortality.", "gemini": "BỐI CẢNH Một loại coronavirus mới (hội chứng hô hấp cấp tính nặng do coronavirus 2 [SARS-CoV-2]) đã xuất hiện tại Trung Quốc vào tháng 12 năm 2019 và đã lây lan trên toàn cầu. Trong nghiên cứu này, chúng tôi nhằm mục đích mô tả các đặc điểm lâm sàng và kết quả của những người lớn tuổi nhập viện mắc bệnh do coronavirus năm 2019 (COVID-19) tại Thổ Nhĩ Kỳ. PHƯƠNG PHÁP Chúng tôi đã phân tích hồi cứu dữ liệu lâm sàng của bệnh nhân nhập viện từ 60 tuổi trở lên được xác nhận mắc COVID-19 từ ngày 11 tháng 3 năm 2020 đến ngày 27 tháng 5 năm 2020 bằng cách sử dụng cơ sở dữ liệu sức khỏe toàn quốc. KẾT QUẢ Trong nhóm bệnh nhân toàn quốc này, tổng cộng có 16.942 người lớn tuổi nhập viện mắc COVID-19 đã được ghi danh, trong đó 8.635 người (51%) là nữ. Tuổi trung bình là 71,2 ± 8,5 tuổi, trong khoảng từ 60 đến 113 tuổi. Tỷ lệ tử vong trước và sau lệnh giới nghiêm có sự khác biệt đáng kể về mặt thống kê (lần lượt là 32,2% so với 17,9%; p <0,001). Thông qua phân tích đa biến về nguyên nhân tử vong ở bệnh nhân lớn tuổi, chúng tôi thấy rằng giới tính nam, đái tháo đường, suy tim, mất trí nhớ đều có liên quan đáng kể đến tử vong trong toàn bộ nhóm bệnh nhân. Ngoài các yếu tố nguy cơ đã đề cập ở trên, ở những bệnh nhân từ 60 đến 79 tuổi, tăng huyết áp, bệnh thận mãn tính và ung thư và ở những bệnh nhân từ 80 tuổi trở lên, suy dinh dưỡng cũng có liên quan đến nguy cơ tử vong tăng. KẾT LUẬN Ngoài các kết quả của các nghiên cứu trước có quy mô mẫu nhỏ hơn, kết quả của chúng tôi đã xác nhận mối quan hệ liên quan đến tuổi giữa các bệnh đi kèm cụ thể và tỷ lệ tử vong liên quan đến COVID-19."} +{"idx": 1871042, "en": "MOTIVATION Causal biological interaction networks represent cellular regulatory pathways. Their fusion with other biological data enables insights into disease mechanisms and novel opportunities for drug discovery. RESULTS We developed Causal Network of Diseases (CaNDis), a web server for the exploration of a human causal interaction network, which we expanded with data on diseases and FDA-approved drugs, on the basis of which we constructed a disease-disease network in which the links represent the similarity between diseases. We show how CaNDis can be used to identify candidate genes with known and novel roles in disease co-occurrence and drug-drug interactions. AVAILABILITYAND IMPLEMENTATION CaNDis is freely available to academic users at http://candis.ijs.si and http://candis.insilab.org. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.", "gemini": "ĐỘNG LỰC Các mạng tương tác sinh học có tính nhân quả đại diện cho các con đường điều hòa tế bào. Sự kết hợp của chúng với các dữ liệu sinh học khác cho phép hiểu sâu hơn về cơ chế bệnh và các cơ hội mới để phát hiện thuốc. KẾT QUẢ Chúng tôi đã phát triển Mạng lưới bệnh nhân quả (CaNDis), một máy chủ web để khám phá mạng tương tác nhân quả của con người, mà chúng tôi đã mở rộng với dữ liệu về bệnh tật và các loại thuốc được FDA chấp thuận, trên cơ sở đó, chúng tôi đã xây dựng một mạng lưới bệnh-bệnh trong đó các liên kết đại diện cho sự giống nhau giữa các bệnh. Chúng tôi chỉ ra cách CaNDis có thể được sử dụng để xác định các gen ứng viên có vai trò đã biết và mới trong sự đồng mắc bệnh và tương tác thuốc-thuốc. KHẢ DỤNG VÀ TRIỂN KHAI CaNDis miễn phí cho người dùng học thuật tại http://candis.ijs.si và http://candis.insilab.org. THÔNG TIN BỔ SUNG Dữ liệu bổ sung có sẵn trực tuyến trên Bioinformatics."} +{"idx": 1871043, "en": "Atrial fibrillation (AF) and chronic kidney disease (CKD) are closely related conditions with shared risk factors. The growing prevalence of both AF and CKD indicates that more patients will suffer from concurrent conditions. There are various complex interlinking mechanisms with important implications for the management of these patients. Furthermore, there is uncertainty regarding the use of oral anticoagulation (OAC) in AF and CKD that is reflected by a lack of consensus between international guidelines. Therefore, the importance of understanding the implications of co-existing AF and CKD should not be underestimated. In this review, we discuss the pathophysiology and association between AF and CKD, including the underlying mechanisms, risk of thrombo-embolic and bleeding complications, influence on stroke management, and evidence surrounding the use of OAC for stroke prevention.", "gemini": "Rung nhĩ (AF) và bệnh thận mãn tính (CKD) là những tình trạng có liên quan chặt chẽ với các yếu tố nguy cơ chung. Tỷ lệ mắc AF và CKD ngày càng tăng cho thấy rằng sẽ có nhiều bệnh nhân mắc các tình trạng đồng thời. Có nhiều cơ chế liên kết phức tạp với những ý nghĩa quan trọng đối với việc quản lý những bệnh nhân này. Hơn nữa, vẫn còn nhiều điều không chắc chắn liên quan đến việc sử dụng thuốc chống đông đường uống (OAC) ở AF và CKD được phản ánh bởi việc thiếu sự đồng thuận giữa các hướng dẫn quốc tế. Do đó, tầm quan trọng của việc hiểu các ý nghĩa của AF và CKD cùng tồn tại không nên bị đánh giá thấp. Trong bài đánh giá này, chúng tôi thảo luận về bệnh sinh và mối liên quan giữa AF và CKD, bao gồm các cơ chế cơ bản, nguy cơ biến chứng huyết khối-thuyên tắc và chảy máu, ảnh hưởng đến việc quản lý đột quỵ và bằng chứng xung quanh việc sử dụng OAC để phòng ngừa đột quỵ."} +{"idx": 1871044, "en": "MOTIVATION Record Linkage has versatile applications in real-world data analysis contexts, where several data sets need to be linked on the record level in the absence of any exact identifier connecting related records. An example are medical databases of patients, spread across institutions, that have to be linked on personally identifiable entries like name, date of birth or ZIP code. At the same time, privacy laws may prohibit the exchange of this personally identifiable information (PII) across institutional boundaries, ruling out the outsourcing of the record linkage task to a trusted third party. We propose to employ privacy-preserving record linkage (PPRL) techniques that prevent, to various degrees, the leakage of PII while still allowing for the linkage of related records. RESULTS We develop a framework for fault-tolerant PPRL using secure multi-party computation with the medical record keeping software Mainzelliste as the data source. Our solution does not rely on any trusted third party and all PII is guaranteed to not leak under common cryptographic security assumptions. Benchmarks show the feasibility of our approach in realistic networking settings: linkage of a patient record against a database of 10.000 records can be done in 48s over a heavily delayed (100ms) network connection, or 3.9s with a low-latency connection. AVAILABILITY AND IMPLEMENTATION The source code of the sMPC node is freely available on Github at https://github.com/medicalinformatics/SecureEpilinker subject to the AGPLv3 license. The source code of the modified Mainzelliste is available at https://github.com/medicalinformatics/MainzellisteSEL.", "gemini": "ĐỘNG LỰC Liên kết hồ sơ có nhiều ứng dụng linh hoạt trong bối cảnh phân tích dữ liệu thực tế, trong đó một số tập dữ liệu cần được liên kết ở cấp độ hồ sơ khi không có bất kỳ mã định danh chính xác nào kết nối các hồ sơ liên quan. Một ví dụ là cơ sở dữ liệu y tế của bệnh nhân, được phân bổ trên nhiều cơ sở, phải được liên kết với các mục nhập nhận dạng cá nhân như tên, ngày sinh hoặc mã ZIP. Đồng thời, luật bảo mật có thể cấm việc trao đổi thông tin nhận dạng cá nhân (PII) này giữa các ranh giới của cơ sở, loại trừ việc thuê ngoài nhiệm vụ liên kết hồ sơ cho bên thứ ba đáng tin cậy. Chúng tôi đề xuất sử dụng các kỹ thuật liên kết hồ sơ bảo vệ quyền riêng tư (PPRL) để ngăn chặn ở các mức độ khác nhau việc rò rỉ PII trong khi vẫn cho phép liên kết các hồ sơ liên quan. KẾT QUẢ Chúng tôi phát triển một khuôn khổ cho PPRL chịu lỗi bằng cách sử dụng tính toán đa bên an toàn với phần mềm lưu giữ hồ sơ y tế Mainzelliste làm nguồn dữ liệu. Giải pháp của chúng tôi không dựa vào bất kỳ bên thứ ba đáng tin cậy nào và tất cả PII đều được đảm bảo không bị rò rỉ theo các giả định bảo mật mật mã thông thường. Các điểm chuẩn cho thấy tính khả thi của phương pháp tiếp cận của chúng tôi trong các cài đặt mạng thực tế: có thể liên kết hồ sơ bệnh nhân với cơ sở dữ liệu gồm 10.000 hồ sơ trong vòng 48 giây qua kết nối mạng bị chậm trễ nhiều (100ms) hoặc 3,9 giây với kết nối có độ trễ thấp. TÍNH KHẢ DỤNG VÀ TRIỂN KHAI Mã nguồn của nút sMPC có sẵn miễn phí trên Github tại https://github.com/medicalinformatics/SecureEpilinker theo giấy phép AGPLv3. Mã nguồn của Mainzelliste đã sửa đổi có sẵn tại https://github.com/medicalinformatics/MainzellisteSEL."} +{"idx": 1871045, "en": "MOTIVATION The 2019 novel coronavirus outbreak has significantly affected global health and society. Thus, predicting biological function from pathogen sequence is crucial and urgently needed. However, little work has been conducted to identify viruses by the enzymes that they encode, and which are key to pathogen propagation. RESULTS We built a comprehensive scientific resource, SARS2020, which integrates coronavirus-related research, genomic sequences and results of anti-viral drug trials. In addition, we built a consensus sequence-catalytic function model from which we identified the novel coronavirus as encoding the same proteinase as the severe acute respiratory syndrome virus. This data-driven sequence-based strategy will enable rapid identification of agents responsible for future epidemics. AVAILABILITYAND IMPLEMENTATION SARS2020 is available at http://design.rxnfinder.org/sars2020/. SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.", "gemini": "ĐỘNG LỰC Sự bùng phát của virus corona mới năm 2019 đã ảnh hưởng đáng kể đến sức khỏe và xã hội toàn cầu. Do đó, việc dự đoán chức năng sinh học từ trình tự mầm bệnh là rất quan trọng và cấp thiết. Tuy nhiên, rất ít công trình được tiến hành để xác định vi-rút bằng các enzym mà chúng mã hóa và đóng vai trò quan trọng trong quá trình lan truyền mầm bệnh. KẾT QUẢ Chúng tôi đã xây dựng một nguồn khoa học toàn diện, SARS2020, tích hợp nghiên cứu liên quan đến vi-rút corona, trình tự bộ gen và kết quả thử nghiệm thuốc chống vi-rút. Ngoài ra, chúng tôi đã xây dựng một mô hình chức năng trình tự đồng thuận mà từ đó chúng tôi xác định được vi-rút corona mới mã hóa cùng một loại proteinase như vi-rút hội chứng hô hấp cấp tính nặng. Chiến lược dựa trên trình tự do dữ liệu này sẽ cho phép xác định nhanh chóng các tác nhân gây ra các dịch bệnh trong tương lai. TÍNH KHẢ DỤNG VÀ TRIỂN KHAI SARS2020 có sẵn tại http://design.rxnfinder.org/sars2020/. THÔNG TIN BỔ SUNG Dữ liệu bổ sung có sẵn trực tuyến tại Bioinformatics."} +{"idx": 1871046, "en": "INTRODUCTION The HEART (history, electrocardiogram [ECG], age, risk factors, troponin) pathway is a useful tool in the emergency department to identify patients that are safe for outpatient evaluation of chest pain. A dedicated HEART Clinic to follow-up versus primary care remains a topic that requires further delineation. We sought to identify how many patients discharged on the HEART pathway specifically followed up with the established HEART Clinic. MATERIALS AND METHODS This is a secondary analysis of a previously published dataset. In an initial validation study of the HEART Pathway, 625 consecutive subjects were identified via chart review, 449 of which were included. We identified subjects for inclusion in this study if they were found to have a HEART score of 3 or less. Subjects were excluded if they were admitted or if their follow-up was beyond 6 weeks. RESULTS Of the 449 subjects, 185 met criteria for study inclusion. 125 (67.6%) had follow-up with an average time of 7.94 days (95% CI: 6.54-9.34). Of those, half had additional testing such as ECG, cardiac computed tomography angiography, and treadmill stress testing. The most common clinics for follow-up were the Family Medicine, Internal Medicine, and HEART Clinic representing 35.8, 29, and 18% of the follow-ups, respectively. No subject died, had a myocardial infarction, or required reperfusion. CONCLUSIONS Of the subjects discharged on the HEART Pathway, 67.6% followed up. Of those subjects that followed up, 18% did so at the HEART Clinic.", "gemini": "GIỚI THIỆU Con đường HEART (tiền sử, điện tâm đồ [ECG], tuổi, các yếu tố nguy cơ, troponin) là một công cụ hữu ích trong khoa cấp cứu để xác định những bệnh nhân có thể được đánh giá ngoại trú về đau ngực. Một Phòng khám HEART chuyên biệt để theo dõi so với chăm sóc chính vẫn là một chủ đề đòi hỏi phải được mô tả thêm. Chúng tôi tìm cách xác định có bao nhiêu bệnh nhân được xuất viện trên con đường HEART được theo dõi cụ thể tại Phòng khám HEART đã thành lập. VẬT LIỆU VÀ PHƯƠNG PHÁP Đây là một phân tích thứ cấp của một tập dữ liệu đã được công bố trước đó. Trong một nghiên cứu xác nhận ban đầu về Đường dẫn HEART, 625 đối tượng liên tiếp đã được xác định thông qua xem xét biểu đồ, trong đó có 449 đối tượng được đưa vào. Chúng tôi xác định các đối tượng để đưa vào nghiên cứu này nếu họ được phát hiện có điểm số HEART là 3 hoặc ít hơn. Các đối tượng bị loại trừ nếu họ được nhập viện hoặc nếu thời gian theo dõi của họ quá 6 tuần. KẾT QUẢ Trong số 449 đối tượng, 185 đối tượng đáp ứng tiêu chí để đưa vào nghiên cứu. 125 (67,6%) đã được theo dõi với thời gian trung bình là 7,94 ngày (95% CI: 6,54-9,34). Trong số đó, một nửa đã được kiểm tra thêm như ECG, chụp động mạch vành cắt lớp vi tính tim và kiểm tra gắng sức trên máy chạy bộ. Các phòng khám phổ biến nhất để theo dõi là Y học gia đình, Nội khoa và Phòng khám HEART, lần lượt chiếm 35,8, 29 và 18% các lần theo dõi. Không có đối tượng nào tử vong, bị nhồi máu cơ tim hoặc cần phải tái tưới máu. KẾT LUẬN Trong số các đối tượng được xuất viện trên Đường dẫn HEART, 67,6% đã được theo dõi. Trong số những đối tượng được theo dõi, 18% đã làm như vậy tại Phòng khám HEART."} +{"idx": 1871047, "en": "MOTIVATION The microbes that live in an environment can be identified from the combined genomic material, also referred to as the metagenome. Sequencing a metagenome can result in large volumes of sequencing reads. A promising approach to reduce the size of metagenomic datasets is by clustering reads into groups based on their overlaps. Clustering reads are valuable to facilitate downstream analyses, including computationally intensive strain-aware assembly. As current read clustering approaches cannot handle the large datasets arising from high-throughput metagenome sequencing, a novel read clustering approach is needed. In this article, we propose OGRE, an Overlap Graph-based Read clustEring procedure for high-throughput sequencing data, with a focus on shotgun metagenomes. RESULTS We show that for small datasets OGRE outperforms other read binners in terms of the number of species included in a cluster, also referred to as cluster purity, and the fraction of all reads that is placed in one of the clusters. Furthermore, OGRE is able to process metagenomic datasets that are too large for other read binners into clusters with high cluster purity. CONCLUSION OGRE is the only method that can successfully cluster reads in species-specific clusters for large metagenomic datasets without running into computation time- or memory issues. AVAILABILITYAND IMPLEMENTATION Code is made available on Github (https://github.com/Marleen1/OGRE). SUPPLEMENTARY INFORMATION Supplementary data are available at Bioinformatics online.", "gemini": "ĐỘNG LỰC Các vi khuẩn sống trong một môi trường có thể được xác định từ vật liệu bộ gen kết hợp, còn được gọi là metagenome. Việc giải trình tự một metagenome có thể tạo ra khối lượng lớn các lần đọc trình tự. Một cách tiếp cận đầy hứa hẹn để giảm kích thước của bộ dữ liệu metagenomic là nhóm các lần đọc thành các nhóm dựa trên sự chồng lấp của chúng. Nhóm các lần đọc có giá trị để tạo điều kiện thuận lợi cho các phân tích hạ nguồn, bao gồm lắp ráp nhận biết chủng tốn nhiều tính toán. Vì các phương pháp nhóm các lần đọc hiện tại không thể xử lý các bộ dữ liệu lớn phát sinh từ giải trình tự metagenome thông lượng cao, nên cần có một phương pháp nhóm các lần đọc mới. Trong bài viết này, chúng tôi đề xuất OGRE, một quy trình nhóm các lần đọc dựa trên Đồ thị chồng lấp cho dữ liệu giải trình tự thông lượng cao, tập trung vào các metagenome shotgun. KẾT QUẢ Chúng tôi chỉ ra rằng đối với các bộ dữ liệu nhỏ, OGRE hoạt động tốt hơn các trình phân loại đọc khác về số lượng loài có trong một nhóm, còn được gọi là độ tinh khiết của nhóm và tỷ lệ tất cả các lần đọc được đặt trong một trong các nhóm. Hơn nữa, OGRE có khả năng xử lý các bộ dữ liệu metagenomic quá lớn đối với các trình phân loại đọc khác thành các nhóm có độ tinh khiết nhóm cao. KẾT LUẬN OGRE là phương pháp duy nhất có thể nhóm các lần đọc thành công trong các nhóm cụ thể cho các bộ dữ liệu metagenomic lớn mà không gặp phải các vấn đề về thời gian tính toán hoặc bộ nhớ. TÍNH KHẢ DỤNG VÀ TRIỂN KHAI Mã được cung cấp trên Github (https://github.com/Marleen1/OGRE). THÔNG TIN BỔ SUNG Dữ liệu bổ sung có sẵn trực tuyến tại Bioinformatics."} +{"idx": 1871048, "en": "The distribution of fitness effects (DFEs) of new mutations across different environments quantifies the potential for adaptation in a given environment and its cost in others. So far, results regarding the cost of adaptation across environments have been mixed, and most studies have sampled random mutations across different genes. Here, we quantify systematically how costs of adaptation vary along a large stretch of protein sequence by studying the distribution of fitness effects of the same ≈2,300 amino-acid changing mutations obtained from deep mutational scanning of 119 amino acids in the middle domain of the heat shock protein Hsp90 in five environments. This region is known to be important for client binding, stabilization of the Hsp90 dimer, stabilization of the N-terminal-Middle and Middle-C-terminal interdomains, and regulation of ATPase-chaperone activity. Interestingly, we find that fitness correlates well across diverse stressful environments, with the exception of one environment, diamide. Consistent with this result, we find little cost of adaptation; on average only one in seven beneficial mutations is deleterious in another environment. We identify a hotspot of beneficial mutations in a region of the protein that is located within an allosteric center. The identified protein regions that are enriched in beneficial, deleterious, and costly mutations coincide with residues that are involved in the stabilization of Hsp90 interdomains and stabilization of client-binding interfaces, or residues that are involved in ATPase-chaperone activity of Hsp90. Thus, our study yields information regarding the role and adaptive potential of a protein sequence that complements and extends known structural information.", "gemini": "Phân bố các hiệu ứng về độ phù hợp (DFE) của các đột biến mới trên các môi trường khác nhau định lượng tiềm năng thích nghi trong một môi trường nhất định và chi phí của nó ở những môi trường khác. Cho đến nay, các kết quả liên quan đến chi phí thích nghi trên các môi trường vẫn còn hỗn tạp và hầu hết các nghiên cứu đều lấy mẫu các đột biến ngẫu nhiên trên các gen khác nhau. Ở đây, chúng tôi định lượng một cách có hệ thống cách các chi phí thích nghi thay đổi dọc theo một đoạn dài của trình tự protein bằng cách nghiên cứu sự phân bố các hiệu ứng về độ phù hợp của cùng ≈2.300 đột biến thay đổi axit amin thu được từ quá trình quét đột biến sâu của 119 axit amin trong miền giữa của protein sốc nhiệt Hsp90 trong năm môi trường. Vùng này được biết là quan trọng đối với sự liên kết của khách hàng, sự ổn định của dimer Hsp90, sự ổn định của các miền xen kẽ N-đầu-Giữa và Giữa-C-đầu và điều chỉnh hoạt động của ATPase-chaperone. Điều thú vị là chúng tôi thấy rằng độ phù hợp tương quan tốt trên các môi trường căng thẳng khác nhau, ngoại trừ một môi trường, diamide. Phù hợp với kết quả này, chúng tôi thấy ít chi phí thích nghi; trung bình chỉ một trong bảy đột biến có lợi là có hại trong một môi trường khác. Chúng tôi xác định một điểm nóng của các đột biến có lợi trong một vùng của protein nằm trong một trung tâm dị lập. Các vùng protein được xác định có nhiều đột biến có lợi, có hại và tốn kém trùng với các phần tử tham gia vào quá trình ổn định các miền xen kẽ Hsp90 và ổn định các giao diện liên kết khách hàng hoặc các phần tử tham gia vào hoạt động ATPase-chaperone của Hsp90. Do đó, nghiên cứu của chúng tôi cung cấp thông tin về vai trò và tiềm năng thích nghi của một trình tự protein bổ sung và mở rộng thông tin cấu trúc đã biết."} +{"idx": 1871049, "en": "OBJECTIVE To describe the effectiveness and risk management of remote rehabilitation for coronavirus disease (COVID-19) patients. DESIGN Single-centre, retrospective, observational study. PATIENTS COVID-19 patients undergoing rehabilitation (24 April to 24 May 2020). METHODS All COVID-19 inpatients undergoing rehabilitation in the general ward were assessed. Data were collected on age, sex, physical ability, rehabilitation modality (remote/direct), need for intubation or extracorporeal membrane oxygenation, degree of pneumonia, oxygen therapy from the start of rehabilitation, D-dimer and C-reactive protein levels, and rehabilitation-related complications. Activities of daily living were measured using the Barthel Index. RESULTS Out of a total of 43 patients, 14 were initially provided with remote rehabilitation and 29 with direct rehabilitation. Four patients were switched from direct to remote rehabilitation during the study, thus at the end of the study there were 18 in the remote rehabilitation group and 25 in the direct rehabilitation group. Patients in remote rehabilitation were significantly younger than those in direct rehabilitation. Of 12 patients who required intubation, 3 were given remote rehabilitation. One extracorporeal membrane oxygenation survivor underwent direct rehabilitation. All patients on remote rehabilitation were discharged home or to a hotel. Twelve out of 29 patients were transferred to a rehabilitation hospital due to delayed recovery of activities of daily living. No serious adverse events occurred. CONCLUSION Effective and safe remote rehabilitation was performed in 41.9% of COVID-19 patients in this study, which resulted in improved rehabilitation in COVID-19 zones.", "gemini": "MỤC TIÊU Mô tả hiệu quả và quản lý rủi ro của phục hồi chức năng từ xa cho bệnh nhân mắc bệnh do virus corona (COVID-19). THIẾT KẾ Nghiên cứu quan sát hồi cứu, một trung tâm. BỆNH NHÂN Bệnh nhân COVID-19 đang phục hồi chức năng (từ ngày 24 tháng 4 đến ngày 24 tháng 5 năm 2020). PHƯƠNG PHÁP Tất cả bệnh nhân nội trú mắc COVID-19 đang phục hồi chức năng ở khoa tổng quát đều được đánh giá. Dữ liệu được thu thập về tuổi, giới tính, khả năng thể chất, phương thức phục hồi chức năng (từ xa/trực tiếp), nhu cầu đặt nội khí quản hoặc oxy hóa qua màng ngoài cơ thể, mức độ viêm phổi, liệu pháp oxy từ khi bắt đầu phục hồi chức năng, mức D-dimer và protein phản ứng C, và các biến chứng liên quan đến phục hồi chức năng. Các hoạt động hàng ngày được đo bằng Chỉ số Barthel. KẾT QUẢ Trong tổng số 43 bệnh nhân, 14 bệnh nhân ban đầu được phục hồi chức năng từ xa và 29 bệnh nhân được phục hồi chức năng trực tiếp. Bốn bệnh nhân được chuyển từ phục hồi chức năng trực tiếp sang phục hồi chức năng từ xa trong quá trình nghiên cứu, do đó vào cuối nghiên cứu có 18 bệnh nhân trong nhóm phục hồi chức năng từ xa và 25 bệnh nhân trong nhóm phục hồi chức năng trực tiếp. Bệnh nhân phục hồi chức năng từ xa trẻ hơn đáng kể so với những bệnh nhân phục hồi chức năng trực tiếp. Trong số 12 bệnh nhân phải đặt nội khí quản, có 3 bệnh nhân được phục hồi chức năng từ xa. Một người sống sót sau quá trình oxy hóa qua màng ngoài cơ thể đã được phục hồi chức năng trực tiếp. Tất cả bệnh nhân phục hồi chức năng từ xa đều được xuất viện về nhà hoặc đến khách sạn. Mười hai trong số 29 bệnh nhân được chuyển đến bệnh viện phục hồi chức năng do phục hồi các hoạt động hàng ngày bị chậm trễ. Không có biến cố bất lợi nghiêm trọng nào xảy ra. KẾT LUẬN Phục hồi chức năng từ xa hiệu quả và an toàn đã được thực hiện ở 41,9% bệnh nhân COVID-19 trong nghiên cứu này, giúp cải thiện phục hồi chức năng ở các khu vực có COVID-19."} +{"idx": 1871050, "en": "Maleimides are essential compounds for drug conjugation reactions via thiols to antibodies, peptides and other targeting units. However, one main drawback is the occurrence of thiol exchange reactions with, for example, glutathione resulting in loss of the targeting ability. A new strategy to overcome such retro-Michael exchange processes of maleimide-thiol conjugates by stabilization of the thiosuccinimide via a transcyclization reaction is presented. This reaction enables the straightforward synthesis of stable maleimide-thiol adducts essential in drug-conjugation applications.", "gemini": "Maleimide là hợp chất thiết yếu cho phản ứng liên hợp thuốc thông qua nhóm thiol với kháng thể, peptide và các đơn vị nhắm mục tiêu khác. Tuy nhiên, một nhược điểm chính là sự xảy ra các phản ứng trao đổi thiol với, ví dụ, glutathione dẫn đến mất khả năng nhắm mục tiêu. Một chiến lược mới để khắc phục quá trình trao đổi retro-Michael như vậy của liên hợp maleimide-thiol bằng cách ổn định thiosuccinimide thông qua phản ứng chuyển vòng được trình bày. Phản ứng này cho phép tổng hợp trực tiếp các chất kết hợp maleimide-thiol ổn định cần thiết trong các ứng dụng liên hợp thuốc."} +{"idx": 1871051, "en": "The synthesis of pristine non-planar nanographenes (NGs) via a cyclodehydrofluorination strategy is reported and the creation of highly strained systems via alumina-assisted C-F bond activation is shown. Steric hindrance could execute an alternative coupling program leading to rare octagon formation offering access to elusive non-classical NGs. The combination of two alternative ways of folding could lead to the formation of various 3D NG objects, resembling the Japanese art of origami. The power of the presented \"origami\" approach is proved by the assembly of 12 challenging nanographenes that are π-isoelectronic to planar hexabenzocoronene but forced out of planarity.", "gemini": "Việc tổng hợp các nanographen không phẳng nguyên sơ (NG) thông qua chiến lược cyclodehydrofluorination được báo cáo và việc tạo ra các hệ thống có ứng suất cao thông qua hoạt hóa liên kết C-F hỗ trợ bằng alumina được thể hiện. Sự cản trở không gian có thể thực hiện một chương trình liên kết thay thế dẫn đến sự hình thành bát giác hiếm, mở ra khả năng tiếp cận các NG phi cổ điển khó nắm bắt. Sự kết hợp của hai cách gấp thay thế có thể dẫn đến sự hình thành của nhiều vật thể NG 3D, giống với nghệ thuật gấp giấy origami của Nhật Bản. Sức mạnh của phương pháp \"origami\" được trình bày được chứng minh bằng việc lắp ráp 12 nanographen đầy thách thức có π đồng điện tử với hexabenzocoronene phẳng nhưng bị ép ra khỏi mặt phẳng."} +{"idx": 1871052, "en": "Phasepy is a Python based package for fluid phase equilibria and interfacial properties calculation from equation of state (EoS). Phasepy uses several tools (i.e., NumPy, SciPy, Pandas, Matplotlib) allowing use Phasepy under Jupyter Notebooks. Phasepy models phase equilibria with the traditional ϕ-γ and ϕ-ϕ approaches, where ϕ (fugacity coefficient) can be modeled as a perfect gas, virial gas or EoS fluid, whereas γ (activity coefficient) can be described by conventional models (NRTL, Wilson, Redlich-Kister expansion, and the group contribution modified-UNIFAC). Interfacial properties are based on the square gradient theory couple to ϕ-ϕ approach. The available EoSs are the cubic EoS family extended to mixtures through the quadratic, modified-Huron-Vidal, and Wong-Sandler mixing rules. Phasepy allows to analyze phase stability, compute phase equilibria, interfacial properties, and optimize their parameters for vapor-liquid, liquid-liquid, and vapor-liquid-liquid equilibria for multicomponent mixtures. Phasepy implementation, and robustness are illustrated for binary and ternary mixtures.", "gemini": "Phasepy là một gói dựa trên Python để tính toán cân bằng pha lỏng và tính chất giao diện từ phương trình trạng thái (EoS). Phasepy sử dụng một số công cụ (tức là NumPy, SciPy, Pandas, Matplotlib) cho phép sử dụng Phasepy trong Jupyter Notebooks. Phasepy mô hình hóa cân bằng pha với các phương pháp tiếp cận ϕ-γ và ϕ-ϕ truyền thống, trong đó ϕ (hệ số độ dẻo) có thể được mô hình hóa như một loại khí hoàn hảo, khí virial hoặc chất lỏng EoS, trong khi γ (hệ số hoạt động) có thể được mô tả bằng các mô hình thông thường (NRTL, Wilson, mở rộng Redlich-Kister và nhóm đóng góp sửa đổi-UNIFAC). Các tính chất giao diện dựa trên lý thuyết độ dốc bình phương kết hợp với phương pháp tiếp cận ϕ-ϕ. Các EoS có sẵn là họ EoS khối được mở rộng sang hỗn hợp thông qua các quy tắc trộn bậc hai, Huron-Vidal đã sửa đổi và Wong-Sandler. Phasepy cho phép phân tích độ ổn định pha, tính toán cân bằng pha, tính chất giao diện và tối ưu hóa các thông số của chúng đối với cân bằng hơi-lỏng, lỏng-lỏng và hơi-lỏng-lỏng đối với hỗn hợp đa thành phần. Việc triển khai và độ mạnh mẽ của Phasepy được minh họa cho hỗn hợp nhị phân và tam phân."} +{"idx": 1871053, "en": "OBJECTIVES Intracellular reactive oxygen species (ROS) induced by receptor activator of NF-kB ligand (RANKL) has been proven to be a critical factor in the development of osteoclasts. This study aimed to prove that schisandrin A (Sch), a novel anti-oxidant compound, is able to suppress osteoclastogenesis and prevent bone loss in ovariectomized (OVX) mice by suppressing ROS via nuclear factor erythroid 2-related factor (Nrf2). MATERIAL AND METHODS Micro-CT was used to detect bone formation. The effects of Sch on receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced reactive oxygen species (ROS) were measured by dihydroethidium (DHE) staining in vivo and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) staining in vitro. Immunofluorescence staining was used to detect the expression of Nrf2 in vivo. siRNA was used to evaluate the effect of Nrf2 in osteoclastogenesis. RESULTS Sch suppresses RANKL-induced ROS production by regulating nuclear factor erythroid 2-related factor (Nrf2) in vitro and vivo. Mechanistically, Sch enhances the expression of Nrf2 by regulating the degradation of Nrf2. Further, Sch suppresses phosphorylation of P65 and its nuclear translocation, as well as the degradation of IκBα. Collectively, our findings reveal that Sch protects against OVX-induced bone loss by suppressing ROS via Nrf2. CONCLUSIONS Our results showed the potential of anti-oxidant compound schisandrin A in the treatment of osteoporosis, highlighting Nrf2 as a novel promising target in osteoclast-related disease.", "gemini": "MỤC TIÊU Các loài oxy phản ứng nội bào (ROS) do thụ thể hoạt hóa của phối tử NF-kB (RANKL) gây ra đã được chứng minh là một yếu tố quan trọng trong sự phát triển của các tế bào hủy xương. Nghiên cứu này nhằm chứng minh rằng schisandrin A (Sch), một hợp chất chống oxy hóa mới, có khả năng ức chế sự tạo thành tế bào hủy xương và ngăn ngừa mất xương ở chuột cắt buồng trứng (OVX) bằng cách ức chế ROS thông qua yếu tố liên quan đến nhân erythroid 2 (Nrf2). VẬT LIỆU VÀ PHƯƠNG PHÁP Micro-CT được sử dụng để phát hiện sự hình thành xương. Các tác động của Sch đối với thụ thể hoạt hóa của phối tử nhân tố hạt nhân-κB (NF-κB) (RANKL) gây ra các loài oxy phản ứng (ROS) được đo bằng phương pháp nhuộm dihydroethidium (DHE) in vivo và nhuộm 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in vitro. Phương pháp nhuộm miễn dịch huỳnh quang được sử dụng để phát hiện sự biểu hiện của Nrf2 in vivo. siRNA được sử dụng để đánh giá tác dụng của Nrf2 trong quá trình tạo thành tế bào hủy xương. KẾT QUẢ Sch ức chế sản xuất ROS do RANKL gây ra bằng cách điều chỉnh yếu tố liên quan đến nhân erythroid 2 (Nrf2) in vitro và vivo. Về mặt cơ chế, Sch tăng cường biểu hiện của Nrf2 bằng cách điều chỉnh sự thoái hóa của Nrf2. Hơn nữa, Sch ức chế quá trình phosphoryl hóa của P65 và sự chuyển vị hạt nhân của nó, cũng như sự thoái hóa của IκBα. Nhìn chung, những phát hiện của chúng tôi cho thấy Sch bảo vệ chống lại mất xương do OVX gây ra bằng cách ức chế ROS thông qua Nrf2. KẾT LUẬN Kết quả của chúng tôi cho thấy tiềm năng của hợp chất chống oxy hóa schisandrin A trong điều trị loãng xương, nhấn mạnh Nrf2 là một mục tiêu mới đầy hứa hẹn trong bệnh liên quan đến tế bào hủy xương."} +{"idx": 1871054, "en": "The coordination chemistry and the activities in the ring-opening polymerization catalysis of racemic lactide (LA) of magnesium complexes of a series of {ONNN}-type sequential monoanionic ligands are described. All ligands include pyridyl and substituted-phenolate as peripheral groups. The ligands bearing either chiral or meso-bipyrrolidine cores led to single diastereomeric complexes, whereas the ligands bearing a diaminoethane core led to diastereomer mixtures. All {ONNN}Mg-X complexes [X=Cl, HMDS (hexamethyldisilazide)] led to highly active and isoselective catalysts. The complexes bearing the chiral bipyrrolidine core exhibited the highest activities (full consumption of 5000 equiv. of rac-LA at RT within 5 min) and highest isoselectivities (Pm =0.91), as well as a living character. The complexes of the meso-bipyrrolidine based ligands were almost as active and slightly less stereoselective, while those of the diaminoethane based ligands exhibited reduced activities and isoselectivities.", "gemini": "Hóa học phối hợp và các hoạt động trong quá trình xúc tác trùng hợp mở vòng của lactide racemic (LA) của các phức hợp magiê của một loạt các phối tử đơn anion tuần tự loại {ONNN} được mô tả. Tất cả các phối tử bao gồm pyridyl và phenolat thế làm nhóm ngoại vi. Các phối tử mang lõi bipyrrolidine meso hoặc chiral dẫn đến các phức hợp diastereomeric đơn, trong khi các phối tử mang lõi diaminoethane dẫn đến hỗn hợp diastereomer. Tất cả các phức hợp {ONNN}Mg-X [X=Cl, HMDS (hexamethyldisilazide)] đều dẫn đến các chất xúc tác có hoạt tính cao và chọn lọc đồng phân. Các phức hợp mang lõi bipyrrolidine chiral biểu hiện các hoạt tính cao nhất (tiêu thụ hoàn toàn 5000 tương đương của rac-LA ở RT trong vòng 5 phút) và chọn lọc đồng phân cao nhất (Pm = 0,91), cũng như đặc tính sống. Các phức hợp của các phối tử dựa trên meso-bipyrrolidine hoạt động gần như tương đương và chọn lọc lập thể kém hơn một chút, trong khi các phối tử dựa trên diaminoethane biểu hiện các hoạt động và chọn lọc đồng phân giảm."} +{"idx": 1871055, "en": "PURPOSE The purpose of this work is to describe a T1 -weighted fluid-attenuated inversion recovery (FLAIR) sequence that is able to produce sharp magnetic resonance images even if the subject is moving their head throughout the acquisition. METHODS The robustness to motion artifacts and retrospective motion correction capabilities of the PROPELLER (periodically rotated overlapping parallel lines with enhanced reconstruction) trajectory were combined with prospective motion correction. The prospective correction was done using an intelligent marker attached to the subject. This marker wirelessly synchronizes to the pulse sequence to measure the directionality and magnitude of the magnetic fields present in the MRI machine during a short navigator, thus enabling it to determine its position and orientation in the scanner coordinate frame. Three approaches to incorporating the marker-navigator into the PROPELLER sequence were evaluated. The specific absorption rate, and subsequent scan time, of the T1 -weighted FLAIR PROPELLER sequence, was reduced using a variable refocusing flip-angle scheme. Evaluations of motion correction performance were done with 4 volunteers and 3 types of head motion. RESULTS During minimal out-of-plane movement, retrospective PROPELLER correction performed similarly to the prospective correction. However, the prospective clearly outperformed the retrospective correction when there was out-of-plane motion. Finally, the combination of retrospective and prospective correction produced the sharpest images even during large continuous motion. CONCLUSION Prospective motion correction of a PROPELLER sequence makes it possible to handle continuous, large, and high-speed head motions with only minor reductions in image quality.", "gemini": "MỤC ĐÍCH Mục đích của công trình này là mô tả một chuỗi phục hồi nghịch đảo suy giảm dịch T1 có trọng số (FLAIR) có khả năng tạo ra hình ảnh cộng hưởng từ sắc nét ngay cả khi đối tượng chuyển động đầu trong suốt quá trình thu nhận. PHƯƠNG PHÁP Khả năng chống chịu với các hiện vật chuyển động và khả năng hiệu chỉnh chuyển động hồi cứu của đường quỹ đạo PROPELLER (các đường song song chồng lên nhau quay định kỳ với tái tạo nâng cao) được kết hợp với hiệu chỉnh chuyển động dự đoán. Hiệu chỉnh dự đoán được thực hiện bằng cách sử dụng một điểm đánh dấu thông minh gắn vào đối tượng. Điểm đánh dấu này đồng bộ không dây với chuỗi xung để đo hướng và độ lớn của các trường từ có trong máy MRI trong một bộ định vị ngắn, do đó cho phép xác định vị trí và hướng của nó trong khung tọa độ máy quét. Đã đánh giá ba phương pháp kết hợp điểm đánh dấu-bộ định vị vào chuỗi PROPELLER. Tỷ lệ hấp thụ riêng và thời gian quét tiếp theo của chuỗi PROPELLER FLAIR có trọng số T1 đã được giảm bằng cách sử dụng sơ đồ góc lật lấy nét lại có thể thay đổi. Đánh giá hiệu suất hiệu chỉnh chuyển động được thực hiện với 4 tình nguyện viên và 3 loại chuyển động đầu. KẾT QUẢ Trong quá trình chuyển động tối thiểu ngoài mặt phẳng, hiệu chỉnh PROPELLER hồi cứu thực hiện tương tự như hiệu chỉnh dự đoán. Tuy nhiên, dự đoán rõ ràng vượt trội hơn hiệu chỉnh hồi cứu khi có chuyển động ngoài mặt phẳng. Cuối cùng, sự kết hợp giữa hiệu chỉnh hồi cứu và dự đoán tạo ra hình ảnh sắc nét nhất ngay cả trong quá trình chuyển động liên tục lớn. KẾT LUẬN Hiệu chỉnh chuyển động dự đoán của chuỗi PROPELLER giúp xử lý các chuyển động đầu liên tục, lớn và tốc độ cao với độ giảm chất lượng hình ảnh chỉ nhỏ."} +{"idx": 1871056, "en": "A new diatom genus, Gogorevia, is described on the basis of an integrated molecular and morphological investigation. Two species are described from Vietnam: the generitype, G. rinatii sp. nov., and G. ovalis sp. nov. These species share many morphological features with Achnanthes exigua and allied taxa that were previously transferred to the genera Achnanthidium or Lemnicola. Our current molecular investigation shows that Gogorevia is distinct and phylogenetically removed from the above-mentioned genera. Morphologically, Gogorevia differs from Achnanthidium and Lemnicola by the shape of the valves, possessing uniseriate striae, raphe morphology, and the presence of an evident sternum. The recently described species Lemnicola uniseriata is transferred to Gogorevia on the basis that it possesses the same morphological features and phylogenetic position as other members of the genus.", "gemini": "Một chi tảo cát mới, Gogorevia, được mô tả trên cơ sở một cuộc điều tra tích hợp về phân tử và hình thái. Hai loài được mô tả từ Việt Nam: loài điển hình, G. rinatii sp. nov., và G. ovalis sp. nov. Những loài này có nhiều đặc điểm hình thái giống với Achnanthes exigua và các loài liên quan trước đây được chuyển sang chi Achnanthidium hoặc Lemnicola. Cuộc điều tra phân tử hiện tại của chúng tôi cho thấy Gogorevia là loài riêng biệt và khác biệt về mặt phát sinh chủng loài so với các chi được đề cập ở trên. Về mặt hình thái, Gogorevia khác với Achnanthidium và Lemnicola ở hình dạng của các van, sở hữu các vân đơn hàng, hình thái khe rãnh và sự hiện diện của một xương ức rõ ràng. Loài mới được mô tả gần đây Lemnicola uniseriata được chuyển sang Gogorevia trên cơ sở rằng nó sở hữu các đặc điểm hình thái và vị trí phát sinh chủng loài giống như các thành viên khác của chi."} +{"idx": 1871057, "en": "OBJECTIVES The primate foot has been extensively investigated because of its role in weight-bearing; however, the calcaneus has been relatively understudied. Here we examine entire gorilla calcaneal external shape to understand its relationship with locomotor behavior. MATERIALS AND METHODS Calcanei of Gorilla gorilla gorilla (n = 43), Gorilla beringei graueri (n = 20), and Gorilla beringei beringei (n = 15) were surface or micro-CT scanned. External shape was analyzed through a three-dimensional geometric morphometric sliding semilandmark analysis. Semilandmarks were slid relative to an updated Procrustes average in order to minimize the bending energy of the thin plate spline interpolation function. Shape variation was summarized using principal components analysis of shape coordinates. Procrustes distances between taxa averages were calculated and resampling statistics run to test pairwise differences. Linear measures were collected and regressed against estimated body mass. RESULTS All three taxa exhibit statistically different morphologies (p < .001 for pairwise comparisons). G. g. gorilla demonstrates an anteroposteriorly elongated calcaneus with a deeper cuboid pivot region and mediolaterally flatter posterior talar facet. G. b. beringei possesses the flattest cuboid and most medially-angled posterior talar facets. G. b. graueri demonstrates intermediate articular facet morphology, a medially-angled tuberosity, and an elongated peroneal trochlea. DISCUSSION Articular facet differences separate gorillas along a locomotor gradient. G. g. gorilla is adapted for arboreality with greater joint mobility, while G. b. beringei is adapted for more stereotypical loads associated with terrestriality. G. b. graueri's unique posterolateral morphology may be due to a secondary transition to greater arboreality from a more terrestrial ancestor.", "gemini": "MỤC TIÊU Bàn chân của loài linh trưởng đã được nghiên cứu rộng rãi vì vai trò của nó trong việc chịu trọng lượng; tuy nhiên, xương gót chân vẫn chưa được nghiên cứu nhiều. Ở đây, chúng tôi kiểm tra toàn bộ hình dạng bên ngoài của xương gót chân của loài gorilla để hiểu mối quan hệ của nó với hành vi vận động. VẬT LIỆU VÀ PHƯƠNG PHÁP Xương gót chân của Gorilla gorilla gorilla (n = 43), Gorilla beringei graueri (n = 20) và Gorilla beringei beringei (n = 15) đã được quét bề mặt hoặc vi tính X quang cắt lớp. Hình dạng bên ngoài được phân tích thông qua phân tích trượt bán mốc hình học ba chiều. Các bán mốc được trượt tương đối so với mức trung bình của Procrustes đã cập nhật để giảm thiểu năng lượng uốn của hàm nội suy spline tấm mỏng. Sự thay đổi hình dạng được tóm tắt bằng cách sử dụng phân tích thành phần chính của tọa độ hình dạng. Khoảng cách Procrustes giữa các giá trị trung bình của các loài được tính toán và thống kê lấy mẫu lại để kiểm tra sự khác biệt từng cặp. Các phép đo tuyến tính được thu thập và hồi quy so với khối lượng cơ thể ước tính. KẾT QUẢ Cả ba loài đều thể hiện các hình thái khác biệt về mặt thống kê (p < 0,001 đối với các so sánh từng cặp). G. g. gorilla cho thấy xương gót chân kéo dài trước sau với vùng trục xương khối vuông sâu hơn và mặt tiếp xúc xương sên sau phẳng hơn về mặt trung gian. G. b. beringei sở hữu các mặt tiếp xúc xương khối vuông phẳng nhất và các mặt tiếp xúc xương sên sau có góc giữa nhất. G. b. graueri cho thấy hình thái mặt khớp trung gian, củ xương có góc giữa và ròng rọc xương mác kéo dài. THẢO LUẬN Sự khác biệt về mặt khớp tách biệt các loài gorilla dọc theo một gradient vận động. G. g. gorilla được thích nghi để sống trên cây với khả năng vận động khớp lớn hơn, trong khi G. b. beringei được thích nghi để chịu tải trọng khuôn mẫu hơn liên quan đến tính chất sống trên cạn. Hình thái sau bên đặc biệt của G. b. graueri có thể là do quá trình chuyển đổi thứ cấp sang tính chất sống trên cây nhiều hơn từ một tổ tiên sống trên cạn."} +{"idx": 1871058, "en": "We conducted a prospective, multicenter, randomized, controlled clinical trial to compare the efficacy and safety of high-dose dexamethasone (HD-DXM) plus recombinant human thrombopoietin (rhTPO), vs HD-DXM alone in newly diagnosed adult immune thrombocytopenia (ITP) patients. Enrolled patients were randomly assigned to receive DXM plus rhTPO or DXM monotherapy. Another 4-day course of DXM was repeated if response was not achieved by day 10 in both arms. One hundred patients in the HD-DXM plus rhTPO arm and 96 patients in the HD-DXM monotherapy arm were included in the full analysis set. So, HD-DXM plus rhTPO resulted in a higher incidence of initial response (89.0% vs 66.7%, P < .001) and complete response (CR, 75.0% vs 42.7%, P < .001) compared with HD-DXM monotherapy. Response rate at 6 months was also higher in the HD-DXM plus rhTPO arm than that in the HD-DXM monotherapy arm (51.0% vs 36.5%, P = .02; sustained CR: 46.0% vs 32.3%, P = .043). Throughout the follow-up period, the overall duration of response was greater in the HD-DXM plus rhTPO arm compared to the HD-DXM monotherapy arm (P = .04), as estimated by the Kaplan-Meier analysis. The study drugs were generally well tolerated. In conclusion, the combination of HD-DXM with rhTPO significantly improved the initial response and yielded favorable SR in newly diagnosed ITP patients, thus could be further validated as a frontline treatment for ITP. This study is registered as clinicaltrials.gov identifier: NCT01734044.", "gemini": "Chúng tôi đã tiến hành một thử nghiệm lâm sàng có đối chứng, ngẫu nhiên, đa trung tâm, có triển vọng để so sánh hiệu quả và độ an toàn của dexamethasone liều cao (HD-DXM) cộng với thrombopoietin tái tổ hợp của người (rhTPO) so với HD-DXM đơn trị ở bệnh nhân mắc bệnh giảm tiểu cầu miễn dịch (ITP) ở người lớn mới được chẩn đoán. Những bệnh nhân được ghi danh được chỉ định ngẫu nhiên để nhận DXM cộng với rhTPO hoặc đơn trị DXM. Một đợt DXM 4 ngày khác được lặp lại nếu không đạt được đáp ứng vào ngày thứ 10 ở cả hai nhánh. Một trăm bệnh nhân trong nhóm HD-DXM cộng với rhTPO và 96 bệnh nhân trong nhóm đơn trị HD-DXM đã được đưa vào bộ phân tích đầy đủ. Vì vậy, HD-DXM cộng với rhTPO dẫn đến tỷ lệ đáp ứng ban đầu cao hơn (89,0% so với 66,7%, P < 0,001) và đáp ứng hoàn toàn (CR, 75,0% so với 42,7%, P < 0,001) so với đơn trị HD-DXM. Tỷ lệ đáp ứng ở 6 tháng cũng cao hơn ở nhóm HD-DXM cộng với rhTPO so với nhóm đơn trị HD-DXM (51,0% so với 36,5%, P = 0,02; CR kéo dài: 46,0% so với 32,3%, P = 0,043). Trong suốt thời gian theo dõi, thời gian đáp ứng chung dài hơn ở nhóm HD-DXM cộng với rhTPO so với nhóm đơn trị HD-DXM (P = 0,04), theo ước tính của phân tích Kaplan-Meier. Các loại thuốc nghiên cứu nói chung được dung nạp tốt. Tóm lại, sự kết hợp của HD-DXM với rhTPO đã cải thiện đáng kể đáp ứng ban đầu và mang lại SR thuận lợi ở những bệnh nhân ITP mới được chẩn đoán, do đó có thể được xác nhận thêm là phương pháp điều trị一线 cho ITP. Nghiên cứu này được đăng ký là mã định danh clinicaltrials.gov: NCT01734044."} +{"idx": 1871059, "en": "Influenza D virus (IDV) is a novel orthomyxovirus that was first isolated in 2011 in the United States from a swine exhibiting influenza-like disease. To date, its detection is extended to all continents and in a broad host range: IDV is circulating in cattle, swine, feral swine, camelids, small ruminants and horses. Evidence also suggests a possible species jump to humans, underlining the issue of zoonotic potential. In Europe, serological investigations in cattle have partially allowed the understanding of the virus diffusion in different countries such as Italy, France, Luxembourg and Ireland. The infection is widespread in cattle but limited in other investigated species, consolidating the assumption of cattle as IDV primary host. We hypothesize that commercial livestock trade could play a role in the observed differences in IDV seroprevalence among these areas. Indeed, the overall level of exposure in cattle and swine in destination countries (e.g. Italy) is higher than in origin countries (e.g. France), leading to the hypothesis of a viral shedding following the transportation of young cattle abroad and thus contributing to larger diffusion at countries of destination. IDV large geographic circulation in cattle from Northern to more Southern European countries also supports the hypothesis of a viral spread through livestock trade. This review summarizes available data on IDV seroprevalence in Europe collected so far and integrates unpublished data from IDV European surveillance framework of the last decade. In addition, the possible role of livestock trade and biosecurity measures in this pathogen's spread is discussed.", "gemini": "Vi-rút cúm D (IDV) là một loại vi-rút chỉnh hình mới được phân lập lần đầu tiên vào năm 2011 tại Hoa Kỳ từ một con lợn mắc bệnh giống cúm. Cho đến nay, việc phát hiện ra vi-rút này đã được mở rộng đến tất cả các châu lục và trong phạm vi vật chủ rộng: IDV đang lưu hành ở gia súc, lợn, lợn hoang, lạc đà, động vật nhai lại nhỏ và ngựa. Bằng chứng cũng cho thấy khả năng lây truyền sang người, nhấn mạnh vấn đề về khả năng lây truyền từ động vật sang người. Ở châu Âu, các cuộc điều tra huyết thanh học ở gia súc đã giúp hiểu một phần về sự khuếch tán của vi-rút ở các quốc gia khác nhau như Ý, Pháp, Luxembourg và Ireland. Nhiễm trùng lan rộng ở gia súc nhưng hạn chế ở các loài khác được điều tra, củng cố giả định về gia súc là vật chủ chính của IDV. Chúng tôi đưa ra giả thuyết rằng hoạt động buôn bán gia súc thương mại có thể đóng một vai trò trong sự khác biệt được quan sát thấy về tỷ lệ huyết thanh dương tính với IDV giữa các khu vực này. Thật vậy, mức độ phơi nhiễm chung ở gia súc và lợn ở các quốc gia đích (ví dụ: Ý) cao hơn ở các quốc gia xuất xứ (ví dụ: Pháp), dẫn đến giả thuyết về sự phát tán vi-rút sau khi vận chuyển gia súc non ra nước ngoài và do đó góp phần vào sự lây lan rộng hơn ở các quốc gia đích. Sự lưu hành địa lý rộng lớn của IDV ở gia súc từ các nước Bắc Âu đến Nam Âu cũng ủng hộ giả thuyết về sự lây lan của vi-rút thông qua hoạt động buôn bán gia súc. Tổng quan này tóm tắt dữ liệu có sẵn về tỷ lệ huyết thanh dương tính với IDV ở Châu Âu được thu thập cho đến nay và tích hợp dữ liệu chưa được công bố từ khuôn khổ giám sát IDV của Châu Âu trong thập kỷ qua. Ngoài ra, vai trò có thể có của hoạt động buôn bán gia súc và các biện pháp an toàn sinh học trong quá trình lây lan của mầm bệnh này cũng được thảo luận."} +{"idx": 1871060, "en": "OBJECTIVES Laryngeal dysplasia (LD) is a precancerous lesion of the larynx. In this study, the laryngeal tissue of patients with laryngeal dysplasia was taken as the research object, and the aetiology of reflux was analysed. METHOD Patients with laryngeal dysplasia after surgery were selected as our subjects. The levels of pepsin, enterokinase and bilirubin in laryngeal tissue samples of the two groups were detected by immunohistochemical method. RESULTS The OR values (95% CI) of pepsin, enterokinase and bilirubin were 0.67 (0.19-2.36), 0.80 (0.22-2.98) and 1.33 (0.30-5.96), respectively, in the univariate analysis. Besides, in the multivariate analysis, the OR values (95% CI) of pepsin, enterokinase and bilirubin were 0.57 (0.14-2.30), 0.73 (0.18-2.92) and 1.40 (0.30-6.53), respectively. CONCLUSION Larger sample size should be applied to prospective studies on whether reflux is a risk factor for laryngeal cancer.", "gemini": "MỤC TIÊU Loạn sản thanh quản (LD) là tổn thương tiền ung thư của thanh quản. Trong nghiên cứu này, mô thanh quản của bệnh nhân loạn sản thanh quản được lấy làm đối tượng nghiên cứu và phân tích nguyên nhân trào ngược. PHƯƠNG PHÁP Bệnh nhân loạn sản thanh quản sau phẫu thuật được chọn làm đối tượng nghiên cứu. Nồng độ pepsin, enterokinase và bilirubin trong mẫu mô thanh quản của hai nhóm được phát hiện bằng phương pháp miễn dịch mô hóa học. KẾT QUẢ Các giá trị OR (95% CI) của pepsin, enterokinase và bilirubin lần lượt là 0,67 (0,19-2,36), 0,80 (0,22-2,98) và 1,33 (0,30-5,96) trong phân tích đơn biến. Bên cạnh đó, trong phân tích đa biến, các giá trị OR (95% CI) của pepsin, enterokinase và bilirubin lần lượt là 0,57 (0,14-2,30), 0,73 (0,18-2,92) và 1,40 (0,30-6,53). KẾT LUẬN Cần áp dụng kích thước mẫu lớn hơn cho các nghiên cứu triển vọng về việc trào ngược có phải là yếu tố nguy cơ gây ung thư thanh quản hay không."} +{"idx": 1871061, "en": "Significant technological advances have improved our ability to localize epilepsy and investigate the electrophysiology in patients undergoing preparation for epilepsy surgery. Conversely, our process of decision-making and outcome prediction has remained essentially restricted to subjective clinical judgment. This may have hindered our ability to improve outcomes. In this review, we highlight the cognitive biases that interfere with medical decision-making and present data on the use of algorithms and statistical models in general health care, before pivoting to discuss applications in the context of epilepsy.", "gemini": "Những tiến bộ đáng kể về công nghệ đã cải thiện khả năng định vị động kinh của chúng tôi và điều tra điện sinh lý ở những bệnh nhân đang chuẩn bị phẫu thuật động kinh. Ngược lại, quá trình ra quyết định và dự đoán kết quả của chúng tôi về cơ bản vẫn bị hạn chế trong phạm vi đánh giá lâm sàng chủ quan. Điều này có thể cản trở khả năng cải thiện kết quả của chúng tôi. Trong bài đánh giá này, chúng tôi nêu bật những thành kiến nhận thức cản trở việc ra quyết định y tế và trình bày dữ liệu về việc sử dụng các thuật toán và mô hình thống kê trong chăm sóc sức khỏe nói chung, trước khi chuyển sang thảo luận về các ứng dụng trong bối cảnh động kinh."} +{"idx": 1871062, "en": "BACKGROUND Conventional sequencing uses gene-specific primers to determine the location of RH variants and permits a qualitative assessment of zygosity. Whole-genome and whole-exome sequencing determine the genetic location of variants and enable a quantitative assessment of zygosity. Nonspecific sequencing uses RH-consensus primers to detect variants and sequencing-read ratios to quantify their copy number. STUDY DESIGN AND METHODS Two hundred seventy eight samples with diverse genotypes were analyzed by next-generation sequencing with RH- consensus primers. Custom-developed data analysis software was used to detect individual variants and infer the RH genotype. The method was evaluated for its quantitative nature, its ability to discriminate similar genotypes, its accuracy to detect variants, and its accuracy to assign them to RHD or RHCE. RESULTS As a measure of balanced amplification of RHD and RHCE sequences, observed ratio medians deviate from expected ratios by 3% or less of the ratio range. As a measure of discriminatory power, contiguous RHCE / [RHD + RHCE] ratio averages are separated by 4 or more standard deviations of the mean. Variants are detected with a sensitivity and specificity greater than 99%, and variants at consensus positions are correctly assigned to RHD vs RHCE with a sensitivity greater than 72% and a specificity greater than 99%. The method is successful in the identification of genotypes with large conversion events and in the detection of copy number variation. CONCLUSION Nonspecific sequencing of homologous gene sets combines detection and quantification of genetic variation in a single assay. Evidence is provided for the quantitative nature of the method, its sensitivity and specificity, and its ability to identify complex RH genotypes.", "gemini": "PHƯƠNG NỀN Trình tự thông thường sử dụng các đoạn mồi gen đặc hiệu để xác định vị trí của các biến thể RH và cho phép đánh giá định tính về kiểu gen dị hợp tử. Trình tự toàn bộ hệ gen và toàn bộ exome xác định vị trí di truyền của các biến thể và cho phép đánh giá định lượng về kiểu gen dị hợp tử. Trình tự không đặc hiệu sử dụng các đoạn mồi đồng thuận RH để phát hiện các biến thể và tỷ lệ đọc trình tự để định lượng số bản sao của chúng. THIẾT KẾ NGHIÊN CỨU VÀ PHƯƠNG PHÁP Hai trăm bảy mươi tám mẫu có kiểu gen đa dạng đã được phân tích bằng trình tự thế hệ tiếp theo với các đoạn mồi đồng thuận RH. Phần mềm phân tích dữ liệu tùy chỉnh được sử dụng để phát hiện các biến thể riêng lẻ và suy ra kiểu gen RH. Phương pháp này được đánh giá về bản chất định lượng, khả năng phân biệt các kiểu gen tương tự, độ chính xác trong việc phát hiện các biến thể và độ chính xác trong việc gán chúng cho RHD hoặc RHCE. KẾT QUẢ Là một thước đo khuếch đại cân bằng của các trình tự RHD và RHCE, giá trị trung bình của tỷ lệ quan sát chênh lệch so với tỷ lệ mong đợi không quá 3% phạm vi tỷ lệ. Là một thước đo sức mạnh phân biệt, tỷ lệ trung bình RHCE / [RHD + RHCE] liền kề được phân tách bởi 4 độ lệch chuẩn hoặc nhiều hơn của giá trị trung bình. Các biến thể được phát hiện với độ nhạy và độ đặc hiệu lớn hơn 99% và các biến thể ở các vị trí đồng thuận được gán chính xác cho RHD so với RHCE với độ nhạy lớn hơn 72% và độ đặc hiệu lớn hơn 99%. Phương pháp này thành công trong việc xác định kiểu gen có các sự kiện chuyển đổi lớn và trong việc phát hiện biến thể số bản sao. KẾT LUẬN Trình tự không đặc hiệu của các bộ gen đồng dạng kết hợp phát hiện và định lượng biến thể di truyền trong một xét nghiệm duy nhất. Có bằng chứng về bản chất định lượng của phương pháp, độ nhạy và độ đặc hiệu của nó và khả năng xác định các kiểu gen RH phức tạp."} +{"idx": 1871063, "en": "Pediatric ALF is rare but life-threatening and may require urgent transplantation. In low and middle-income countries, access to transplantation is limited, deceased organ donation rates are low, and data on outcomes scarce. The Wits Donald Gordon Medical Centre, in Johannesburg, is one of only two centers in South Africa that perform pediatric liver transplant. We describe the etiology, clinical presentation, and outcomes of children undergoing liver transplant for ALF at our center over the past 14 years. We performed a retrospective chart review of all children undergoing liver transplantation for ALF from November 2005 to September 2019. Recipient data included demographics, clinical and biochemical characteristics pretransplant, post-operative complications, and survival. We conducted descriptive data analysis and used the Kaplan-Meier method for survival analysis. We performed 182 primary pediatric liver transplants. Of these, 27 (15%) were for ALF, mostly from acute hepatitis A infection (11/27;41%). Just over half of the grafts were from living donors (15/27;56%), and five grafts (5/27;19%) were ABO-incompatible. The most frequent post-transplant complications were biliary leaks (9/27;33%). There were two cases of hepatic artery thrombosis (2/27;7%), one of whom required re-transplantation. Unadjusted patient and graft survival at one and 3 years were the same, at 81% (95% CI 61%-92%) and 78% (95% CI 57%-89%), respectively. At WDGMC, our outcomes for children who undergo liver transplantation for ALF are excellent. We found workable solutions that effectively addressed our pervasive organ shortages without compromising patient outcomes.", "gemini": "ALF nhi khoa rất hiếm nhưng đe dọa tính mạng và có thể cần phải ghép tạng khẩn cấp. Ở các nước thu nhập thấp và trung bình, khả năng tiếp cận ghép tạng bị hạn chế, tỷ lệ hiến tạng từ người đã chết thấp và dữ liệu về kết quả còn khan hiếm. Trung tâm Y tế Wits Donald Gordon, tại Johannesburg, là một trong hai trung tâm duy nhất ở Nam Phi thực hiện ghép gan nhi khoa. Chúng tôi mô tả nguyên nhân, biểu hiện lâm sàng và kết cục của trẻ em trải qua ghép gan vì ALF tại trung tâm của chúng tôi trong 14 năm qua. Chúng tôi đã thực hiện đánh giá biểu đồ hồi cứu đối với tất cả trẻ em trải qua ghép gan vì ALF từ tháng 11 năm 2005 đến tháng 9 năm 2019. Dữ liệu của người nhận bao gồm thông tin nhân khẩu học, đặc điểm lâm sàng và sinh hóa trước khi ghép, các biến chứng sau phẫu thuật và khả năng sống sót. Chúng tôi đã tiến hành phân tích dữ liệu mô tả và sử dụng phương pháp Kaplan-Meier để phân tích khả năng sống sót. Chúng tôi đã thực hiện 182 ca ghép gan nhi khoa chính. Trong số đó, 27 ca (15%) là do ALF, chủ yếu từ nhiễm trùng viêm gan cấp A (11/27; 41%). Chỉ hơn một nửa số ca ghép là từ người cho sống (15/27; 56%) và năm ca ghép (5/27; 19%) không tương thích với ABO. Các biến chứng sau ghép thường gặp nhất là rò đường mật (9/27; 33%). Có hai trường hợp huyết khối động mạch gan (2/27; 7%), một trong số đó phải ghép lại. Tỷ lệ sống sót của bệnh nhân và ca ghép chưa điều chỉnh sau một và 3 năm là như nhau, ở mức 81% (95% CI 61% -92%) và 78% (95% CI 57% -89%), tương ứng. Tại WDGMC, kết quả của chúng tôi đối với trẻ em trải qua ghép gan vì ALF là rất tốt. Chúng tôi đã tìm ra các giải pháp khả thi giải quyết hiệu quả tình trạng thiếu hụt nội tạng tràn lan của mình mà không ảnh hưởng đến kết quả của bệnh nhân."} +{"idx": 1871064, "en": "BACKGROUND The aim of this study was to explore the value of SMI compared with conventional ultrasonography for assessing hepatic arterial blood flow after pediatric liver transplantation. METHODS From March 2018 to November 2018, a total of 105 pediatric recipients with biliary atresia underwent liver transplantation in our hospital. Ultrasound examinations were performed at the bedside in the intensive care unit to check the patency of the blood flow in the hepatic allograft. CDI, PDI, cSMI, and mSMI were performed to assess the display, orientation, and distribution of the graft hepatic artery. Ultrasound examinations were performed by one radiologist, and the images were judged by two observers. RESULTS The median age, weight, and height of the recipients were 6.97 (5.92, 9.58) months, 6.50 (6.00, 7.80) kg, and 64.00 (62.00, 68.00) cm, respectively. The measure of kappa agreement was 0.902, 0.889, 0.882, and 0.882 for CDI, PDI, cSMI, and mSMI, respectively. HAT occurred in 7 pediatric recipients and was confirmed by CTA (computed tomography angiography) and surgery. The diagnostic performance of sensitivity, specificity, PPV (positive predictive value), NPV (negative predictive value), and accuracy were 100%, 92.86%, 50%, 100%, and 93.33% for CDI and 100%, 98.98%, 87.50%, 100%, and 99.05% for SMI. CONCLUSIONS As an additional method to CDI, SMI can clearly show the distribution of hepatic arterial blood flow and provide more details, thereby markedly improving the diagnostic performance of postoperative HAT.", "gemini": "PHƯƠNG NỀN Mục đích của nghiên cứu này là khám phá giá trị của SMI so với siêu âm thông thường để đánh giá lưu lượng máu động mạch gan sau ghép gan nhi khoa. PHƯƠNG PHÁP Từ tháng 3 năm 2018 đến tháng 11 năm 2018, tổng cộng 105 trẻ em nhận ghép do teo đường mật đã được ghép gan tại bệnh viện của chúng tôi. Các xét nghiệm siêu âm được thực hiện tại giường bệnh ở khoa chăm sóc đặc biệt để kiểm tra tính thông suốt của lưu lượng máu trong mảnh ghép gan. CDI, PDI, cSMI và mSMI đã được thực hiện để đánh giá hình ảnh, định hướng và phân bố của động mạch gan ghép. Các xét nghiệm siêu âm được thực hiện bởi một bác sĩ X quang và hình ảnh được đánh giá bởi hai người quan sát. KẾT QUẢ Độ tuổi, cân nặng và chiều cao trung bình của những người nhận là 6,97 (5,92, 9,58) tháng, 6,50 (6,00, 7,80) kg và 64,00 (62,00, 68,00) cm, tương ứng. Đo lường thỏa thuận kappa là 0,902, 0,889, 0,882 và 0,882 cho CDI, PDI, cSMI và mSMI, tương ứng. HAT xảy ra ở 7 trẻ em nhận ghép và được xác nhận bằng CTA (chụp cắt lớp vi tính mạch máu) và phẫu thuật. Hiệu suất chẩn đoán về độ nhạy, độ đặc hiệu, PPV (giá trị dự đoán dương tính), NPV (giá trị dự đoán âm tính) và độ chính xác là 100%, 92,86%, 50%, 100% và 93,33% đối với CDI và 100%, 98,98%, 87,50%, 100% và 99,05% đối với SMI. KẾT LUẬN Là một phương pháp bổ sung cho CDI, SMI có thể hiển thị rõ ràng sự phân bố của lưu lượng máu động mạch gan và cung cấp thêm thông tin chi tiết, do đó cải thiện đáng kể hiệu suất chẩn đoán của HAT sau phẫu thuật."} +{"idx": 1871065, "en": "The use of waste and by-products locally available in large quantities and at low cost as adsorbents can be considered an appropriate approach for improving waste management and protecting the environment. Cotton textile waste was used to prepare adsorbents (MC) via pyrolysis followed by a chemical modification with H3 PO4 . MC samples were characterized by scanning electron microscopy, FTIR spectroscopy, and N2 adsorption-desorption isotherm. The results revealed that MC treated with 1 M H3 PO4 (MC1 ) showed an excellent adsorption performance. The single and binary adsorption of tetracycline (TC) and paracetamol (Pa) onto MC1 were studied. In a single system, TC was better adsorbed than Pa and maximum adsorption capacities qm are 87.7 mg/g and 62 mg/g, respectively. The adsorption follows the Langmuir and pseudo-second-order kinetic models. For a binary system, the experimental data indicate that Pa (44.04 mg/g) is better adsorbed than TC (24.13 mg/g). Adsorption equilibrium data of TC and Pa evaluated by the selectivity extended-Langmuir model in which selectivity factor was introduced provided good correlation results with the binary adsorption data. Cotton textile waste is potentially promising for the preparation of effective adsorbents for the removal of pharmaceutical residues in aqueous solutions. PRACTITIONER POINTS: Valorization of cotton textile waste into adsorbents. Adsorbents were prepared by pyrolysis at 600°C followed by chemical modification in the presence of H3 PO4 . Removal of tetracycline (TC) and paracetamol (Pa) alone or in mixtures by adsorption. Adsorbent showed high-capacity adsorption of the TC and Pa even in a mixture from solutions at low concentrations. The Langmuir and selectivity extended-Langmuir models describe the adsorption of TC and Pa alone and in mixtures, respectively.", "gemini": "Việc sử dụng chất thải và các sản phẩm phụ có sẵn tại địa phương với số lượng lớn và giá thành thấp làm chất hấp phụ có thể được coi là một cách tiếp cận phù hợp để cải thiện việc quản lý chất thải và bảo vệ môi trường. Chất thải dệt may cotton đã được sử dụng để chế tạo chất hấp phụ (MC) thông qua nhiệt phân sau đó là biến đổi hóa học với H3 PO4 . Các mẫu MC được đặc trưng bằng kính hiển vi điện tử quét, quang phổ FTIR và đường đẳng nhiệt hấp phụ-giải hấp N2 . Kết quả cho thấy MC được xử lý bằng 1 M H3 PO4 (MC1 ) cho thấy hiệu suất hấp phụ tuyệt vời. Đã nghiên cứu quá trình hấp phụ đơn và hấp phụ hỗn hợp của tetracycline (TC) và paracetamol (Pa) lên MC1 . Trong một hệ thống đơn, TC được hấp phụ tốt hơn Pa và độ hấp phụ tối đa qm lần lượt là 87,7 mg/g và 62 mg/g. Quá trình hấp phụ tuân theo mô hình động học Langmuir và bậc hai giả. Đối với hệ nhị phân, dữ liệu thực nghiệm chỉ ra rằng Pa (44,04 mg/g) được hấp phụ tốt hơn TC (24,13 mg/g). Dữ liệu cân bằng hấp phụ của TC và Pa được đánh giá bằng mô hình Langmuir mở rộng có chọn lọc trong đó hệ số chọn lọc được đưa vào cung cấp kết quả tương quan tốt với dữ liệu hấp phụ nhị phân. Chất thải dệt may cotton có tiềm năng hứa hẹn trong việc chế tạo chất hấp phụ hiệu quả để loại bỏ cặn dược phẩm trong dung dịch nước. ĐIỂM THỰC HÀNH: Tái sử dụng chất thải dệt may cotton thành chất hấp phụ. Chất hấp phụ được chế tạo bằng phương pháp nhiệt phân ở 600°C sau đó là biến đổi hóa học có sự hiện diện của H3 PO4 . Loại bỏ tetracycline (TC) và paracetamol (Pa) riêng lẻ hoặc trong hỗn hợp bằng cách hấp phụ. Chất hấp phụ cho thấy khả năng hấp phụ cao đối với TC và Pa ngay cả trong hỗn hợp từ các dung dịch ở nồng độ thấp. Các mô hình Langmuir và Langmuir mở rộng có chọn lọc mô tả quá trình hấp phụ của TC và Pa riêng lẻ và trong hỗn hợp."} +{"idx": 1871066, "en": "The syntheses of the homoleptic bis(arene) niobium cations [Nb(arene)2 ]+ (arene = C6 H3 Me3 , C6 H5 Me) with 16 valence electrons and heteroleptic arene-carbonyl cations [(CO)Nb(arene)2 ]+ (arene = C6 H3 Me3 , C6 H5 Me) and [(arene)M(CO)4 ]+ (arene = C6 H3 Me3 , C6 H6 ) obeying 18 valence electrons are described. Stabilization of these complexes was achieved by using the weakly coordinating anions [Al(ORF )4 ]- or [F{Al(ORF )3 }2 ]- (RF = C(CF3 )3 ). The limits of two synthesis routes starting from neutral Nb(arene)2 (arene = C6 H3 Me3 , C6 H5 Me) or [NEt4 ][M(CO)6 ] (M = Nb, Ta) were investigated. All compounds were analyzed by single crystal X-ray determination, vibrational and NMR spectroscopy. DFT calculations were executed to support the experimental data.", "gemini": "Tổng hợp các cation niobi bis(arene) đồng hợp [Nb(arene)2 ]+ (arene = C6 H3 Me3 , C6 H5 Me) với 16 electron hóa trị và cation arene-carbonyl dị hợp [(CO)Nb(arene)2 ]+ (arene = C6 H3 Me3 , C6 H5 Me) và [(arene)M(CO)4 ]+ (arene = C6 H3 Me3 , C6 H6 ) tuân theo 18 electron hóa trị được mô tả. Sự ổn định của các phức chất này đạt được bằng cách sử dụng các anion phối hợp yếu [Al(ORF )4 ]- hoặc [F{Al(ORF )3 }2 ]- (RF = C(CF3 )3 ). Giới hạn của hai con đường tổng hợp bắt đầu từ Nb(arene)2 trung tính (arene = C6 H3 Me3 , C6 H5 Me) hoặc [NEt4 ][M(CO)6 ] (M = Nb, Ta) đã được nghiên cứu. Tất cả các hợp chất đều được phân tích bằng phương pháp xác định cấu trúc tinh thể tia X đơn, quang phổ rung và NMR. Các phép tính DFT đã được thực hiện để hỗ trợ dữ liệu thực nghiệm."} +{"idx": 1871067, "en": "OBJECTIVES To define pre-morbid, clinical, laboratory, and imaging features and identify prognostic factors associated with morbidity and mortality in patients with emphysematous pyelonephritis (EPN) and develop a prognostic scoring system for improving management outcomes. PATIENTS AND METHODS From January 2009 to December 2019, we performed a prospective study of all patients with a suspected diagnosis of EPN referred to a specialist tertiary centre in South India. All patients who underwent non-contrast computed tomography of the abdomen and those diagnosed with EPN were included in this study. Demographic parameters, imaging, haematological and microbiology results were recorded. Patients were divided into three groups: Group 1, patients who survived without any intervention; Group 2, those who survived with surgical intervention; and Group 3, those who died with or without intervention. A prognostic scoring system was developed from 18 different parameters and risk stratification was developed. The scores were correlated with overall prognosis. RESULTS Data from 131 patients with EPN enrolled in the study were analysed: Group 1 (n = 22), Group 2 (n = 102) and Group 3 (n = 7). By using univariate analysis, 10 factors were identified to be significantly associated with prognosis. Diabetes mellitus was the most common comorbidity. Shock at initial admission indicated a poor prognosis and warranted immediate attention (P < 0.001). CONCLUSIONS A multi-disciplinary approach, a high index of clinical suspicion, an early diagnosis and administration of culture-specific antibiotics with identification of prognostic indicators and risk stratification, allows prompt and appropriate medical and surgical treatments that could improve EPN management outcomes.", "gemini": "MỤC TIÊU Xác định các đặc điểm tiền bệnh, lâm sàng, xét nghiệm và hình ảnh và xác định các yếu tố tiên lượng liên quan đến tỷ lệ mắc bệnh và tử vong ở những bệnh nhân mắc viêm thận mủ hoại tử (EPN) và xây dựng hệ thống chấm điểm tiên lượng để cải thiện kết quả điều trị. BỆNH NHÂN VÀ PHƯƠNG PHÁP Từ tháng 1 năm 2009 đến tháng 12 năm 2019, chúng tôi đã tiến hành một nghiên cứu triển vọng đối với tất cả các bệnh nhân được chẩn đoán nghi ngờ mắc EPN được chuyển đến một trung tâm chuyên khoa bậc ba ở Nam Ấn Độ. Tất cả những bệnh nhân được chụp cắt lớp vi tính không cản quang vùng bụng và được chẩn đoán mắc EPN đều được đưa vào nghiên cứu này. Các thông số nhân khẩu học, hình ảnh, kết quả xét nghiệm máu và vi sinh đã được ghi lại. Bệnh nhân được chia thành ba nhóm: Nhóm 1, những bệnh nhân sống sót mà không cần bất kỳ can thiệp nào; Nhóm 2, những bệnh nhân sống sót nhờ can thiệp phẫu thuật; và Nhóm 3, những bệnh nhân tử vong có hoặc không có can thiệp. Một hệ thống chấm điểm tiên lượng đã được phát triển từ 18 thông số khác nhau và phân tầng rủi ro đã được phát triển. Các điểm số có tương quan với tiên lượng chung. KẾT QUẢ Dữ liệu từ 131 bệnh nhân mắc EPN tham gia nghiên cứu đã được phân tích: Nhóm 1 (n = 22), Nhóm 2 (n = 102) và Nhóm 3 (n = 7). Bằng cách sử dụng phân tích đơn biến, 10 yếu tố được xác định có liên quan đáng kể đến tiên lượng. Đái tháo đường là bệnh đi kèm phổ biến nhất. Sốc khi nhập viện ban đầu cho thấy tiên lượng kém và cần được chú ý ngay (P < 0,001). KẾT LUẬN Một cách tiếp cận đa chuyên khoa, một chỉ số cao về nghi ngờ lâm sàng, chẩn đoán sớm và dùng thuốc kháng sinh đặc hiệu theo nuôi cấy với việc xác định các chỉ số tiên lượng và phân tầng rủi ro, cho phép điều trị nội khoa và phẫu thuật kịp thời và phù hợp có thể cải thiện kết quả điều trị EPN."} +{"idx": 1871068, "en": "Reported here is the use of single-layered, chiral porous sheets with induced pore chirality for repeatable asymmetric transformations and self-separation without the need for chiral catalysts or chiral auxiliaries. The asymmetric induction is driven by chiral fixation of absorbed achiral substrates inside the chiral pores for transformation into enantiopure products with enantioselectivities of greater than 99 % ee. When the conversion is completed, the products are spontaneously separated out of the pores, enabling the porous sheets to perform repeated cycles of converting achiral substrates into chiral products for release without compromising pore performance. Confinement of achiral substrates into two-dimensional chiral porous materials provides access to a highly efficient alternative to current asymmetric synthesis methodologies.", "gemini": "Báo cáo ở đây là việc sử dụng các tấm xốp mỏng một lớp, có lỗ xốp có thể thay đổi được với độ xoắn của lỗ được tạo ra để biến đổi không đối xứng có thể lặp lại và tự tách biệt mà không cần chất xúc tác không đối xứng hoặc chất phụ trợ không đối xứng. Sự cảm ứng không đối xứng được thúc đẩy bởi sự cố định không đối xứng của các chất nền không đối xứng được hấp thụ bên trong các lỗ xốp không đối xứng để chuyển đổi thành các sản phẩm tinh khiết quang học với độ chọn lọc quang học lớn hơn 99% ee. Khi quá trình chuyển đổi hoàn tất, các sản phẩm sẽ tự động tách ra khỏi các lỗ xốp, cho phép các tấm xốp thực hiện các chu kỳ chuyển đổi các chất nền không đối xứng thành các sản phẩm không đối xứng để giải phóng mà không ảnh hưởng đến hiệu suất của lỗ xốp. Sự giới hạn các chất nền không đối xứng vào các vật liệu xốp không đối xứng hai chiều giúp tiếp cận một phương pháp thay thế hiệu quả cao cho các phương pháp tổng hợp không đối xứng hiện tại."} +{"idx": 1871069, "en": "OBJECTIVE Macrolide antibiotics are among the most commonly used antibiotics; the association of macrolide antibiotics exposure with sensorineural hearing loss (SNHL) has been hypothesized. A systematic search was conducted in PubMed, EMBASE and Cochrane Library from inception to 15 July 2019 to identify studies used macrolide antibiotics for any indication. The results were reported as odds ratio (OR) with 95% confidence interval (CI) using random-effects model to derive the association of macrolide antibiotics exposure with SNHL. The objective of this meta-analysis was to estimate the association of macrolide antibiotics exposure and SNHL from up-to-date evidence. KEY FINDINGS Nine studies met the inclusion criteria. There was no statistically significant association between macrolide antibiotics exposure and SNHL; the OR was 1.20 (95% CI: 0.96 to 1.49). No significant association was found with any of the subgroup meta-analyses. SUMMARY Whilst the frequency of SNHL was higher with macrolide antibiotics exposure compared with controls, overall, no association was found between macrolide antibiotics and SNHL.", "gemini": "MỤC TIÊU Kháng sinh macrolide là một trong những loại kháng sinh được sử dụng phổ biến nhất; giả thuyết cho rằng việc tiếp xúc với kháng sinh macrolide có liên quan đến mất thính lực do thần kinh cảm giác (SNHL). Một tìm kiếm có hệ thống đã được thực hiện trên PubMed, EMBASE và Thư viện Cochrane từ khi bắt đầu đến ngày 15 tháng 7 năm 2019 để xác định các nghiên cứu sử dụng kháng sinh macrolide cho bất kỳ chỉ định nào. Kết quả được báo cáo dưới dạng tỷ lệ cược (OR) với khoảng tin cậy 95% (CI) sử dụng mô hình hiệu ứng ngẫu nhiên để tìm ra mối liên quan giữa việc tiếp xúc với kháng sinh macrolide với SNHL. Mục tiêu của phân tích tổng hợp này là ước tính mối liên quan giữa việc tiếp xúc với kháng sinh macrolide và SNHL từ các bằng chứng cập nhật. KẾT QUẢ CHÍNH Chín nghiên cứu đáp ứng các tiêu chí tuyển chọn. Không có mối liên quan thống kê đáng kể nào giữa việc tiếp xúc với kháng sinh macrolide và SNHL; OR là 1,20 (KTC 95%: 0,96 đến 1,49). Không tìm thấy mối liên quan đáng kể nào với bất kỳ phân tích tổng hợp nhóm phụ nào. TÓM TẮT Mặc dù tần suất SNHL cao hơn khi tiếp xúc với kháng sinh macrolide so với nhóm đối chứng, nhưng nhìn chung, không tìm thấy mối liên quan nào giữa kháng sinh macrolide và SNHL."} +{"idx": 1871070, "en": "OBJECTIVES Immunodeficient mice injected with human cancer cell lines have been used for human oncology studies and anti-cancer drug trials for several decades. However, rodents are not ideal species for modelling human cancer because rodents are physiologically dissimilar to humans. Therefore, anti-tumour drugs tested effective in rodents have a failure rate of 90% or higher in phase III clinical trials. Pigs are similar to humans in size, anatomy, physiology and drug metabolism rate, rendering them a desirable pre-clinical animal model for assessing anti-cancer drugs. However, xenogeneic immune rejection is a major barrier to the use of pigs as hosts for human tumours. Interleukin (IL)-2 receptor γ (IL2RG), a common signalling subunit for multiple immune cytokines including IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21, is required for proper lymphoid development. MATERIALS AND METHODS IL2RG-/Y pigs were generated by CRISPR/Cas9 technology, and examined for immunodeficiency and ability to support human oncogenesis. RESULTS Compared to age-matched wild-type pigs, IL2RG-/Y pigs exhibited a severely impaired immune system as shown by lymphopenia, lymphoid organ atrophy, poor immunoglobulin function, and T- and NK-cell deficiency. Human melanoma Mel888 cells generated tumours in IL2RG-/Y pigs but not in wild-type littermates. The human tumours grew faster in IL2RG-/Y pigs than in nude mice. CONCLUSIONS Our results indicate that these pigs are promising hosts for modelling human cancer in vivo, which may aid in the discovery and development of anti-cancer drugs.", "gemini": "MỤC TIÊU Chuột suy giảm miễn dịch được tiêm các dòng tế bào ung thư của người đã được sử dụng cho các nghiên cứu về ung thư ở người và thử nghiệm thuốc chống ung thư trong nhiều thập kỷ. Tuy nhiên, loài gặm nhấm không phải là loài lý tưởng để mô hình hóa ung thư ở người vì loài gặm nhấm khác biệt về mặt sinh lý so với con người. Do đó, các loại thuốc chống khối u được thử nghiệm có hiệu quả ở loài gặm nhấm có tỷ lệ thất bại là 90% trở lên trong các thử nghiệm lâm sàng giai đoạn III. Lợn có kích thước, giải phẫu, sinh lý và tốc độ chuyển hóa thuốc tương tự như ở người, khiến chúng trở thành một mô hình động vật tiền lâm sàng mong muốn để đánh giá các loại thuốc chống ung thư. Tuy nhiên, sự đào thải miễn dịch dị loài là một rào cản lớn đối với việc sử dụng lợn làm vật chủ cho các khối u ở người. thụ thể Interleukin (IL) -2 γ (IL2RG), một tiểu đơn vị truyền tín hiệu chung cho nhiều cytokine miễn dịch bao gồm IL-2, IL-4, IL-7, IL-9, IL-15 và IL-21, là cần thiết cho sự phát triển bạch huyết bình thường. VẬT LIỆU VÀ PHƯƠNG PHÁP Lợn IL2RG-/Y được tạo ra bằng công nghệ CRISPR/Cas9 và được kiểm tra về tình trạng suy giảm miễn dịch và khả năng hỗ trợ quá trình ung thư ở người. KẾT QUẢ So với lợn hoang dã cùng tuổi, lợn IL2RG-/Y biểu hiện hệ thống miễn dịch bị suy yếu nghiêm trọng như biểu hiện ở chứng giảm bạch cầu, teo cơ quan bạch huyết, chức năng miễn dịch kém và thiếu tế bào T và NK. Các tế bào Mel888 u hắc tố ở người tạo ra khối u ở lợn IL2RG-/Y nhưng không ở lợn con hoang dã. Các khối u ở người phát triển nhanh hơn ở lợn IL2RG-/Y so với ở chuột không lông. KẾT LUẬN Kết quả của chúng tôi chỉ ra rằng những con lợn này là vật chủ đầy hứa hẹn để mô hình hóa ung thư ở người trong ống nghiệm, có thể hỗ trợ phát hiện và phát triển các loại thuốc chống ung thư."} +{"idx": 1871071, "en": "In view of the considerably high activation energy barrier of the O-O bond formation photocatalytic step in water oxidation, it is essential to understand if and how nonadiabatic factors can accelerate the proton-coupled electron transfer (PCET) rate in this process to find rational design strategies facilitating this step. Herein, constrained ab initio molecular dynamics simulations are performed to investigate this rate-limiting step in a series of catalyst-dye supramolecular complexes functionalized with different alkyl groups on the catalyst component. These structural modifications lead to tunable thermodynamic driving forces, PCET rates, and vibronic coupling with specific resonant torsional modes. These results reveal that such resonant coupling between electronic and nuclear motions contributes to crossing catalytic barriers in PCET reactions by enabling semiclassical coherent conversion of a reactant into a product. Our results provide insight on how to engineer efficient catalyst-dye supramolecular complexes by functionalization with steric substituents for high-performance dye-sensitized photoelectrochemical cells.", "gemini": "Xét đến rào cản năng lượng hoạt hóa khá cao của bước quang xúc tác hình thành liên kết O-O trong quá trình oxy hóa nước, điều cần thiết là phải hiểu các yếu tố phi đoạn nhiệt có thể tăng tốc độ chuyển electron liên hợp proton (PCET) trong quá trình này như thế nào và liệu chúng có thể hay không để tìm ra các chiến lược thiết kế hợp lý tạo thuận lợi cho bước này. Trong bài báo này, các phép tính động lực học phân tử ab initio bị ràng buộc được thực hiện để nghiên cứu bước giới hạn tốc độ này trong một loạt các phức hợp siêu phân tử chất xúc tác-thuốc nhuộm được chức năng hóa bằng các nhóm alkyl khác nhau trên thành phần chất xúc tác. Những sửa đổi về cấu trúc này dẫn đến lực đẩy động lực nhiệt có thể điều chỉnh, tốc độ PCET và liên kết dao động với các chế độ xoắn cộng hưởng cụ thể. Những kết quả này cho thấy rằng sự liên kết cộng hưởng như vậy giữa các chuyển động điện tử và hạt nhân góp phần vượt qua các rào cản xúc tác trong các phản ứng PCET bằng cách cho phép chuyển đổi hợp lý bán cổ điển của chất phản ứng thành sản phẩm. Kết quả của chúng tôi cung cấp cái nhìn sâu sắc về cách thiết kế các phức hợp siêu phân tử chất xúc tác-thuốc nhuộm hiệu quả bằng cách chức năng hóa bằng các chất thế lập thể cho các tế bào quang điện nhạy màu có hiệu suất cao."} +{"idx": 1871072, "en": "Plenty of reports have probed the involvement of abnormally expressed lncRNAs in multiple cancers, including lung squamous cell carcinoma (LUSC). Through online database GEPIA, lncRNA PITPNA antisense RNA 1 (PITPNA-AS1) was highly expressed in LUSC samples, and these tendency was further affirmed in LUSC cells. The aim of current study was to investigate the related mechanism of PITPNA-AS1 in LUSC. Functional experiments verified that depletion of PITPNA-AS1 hampered the proliferative and migratory abilities, but accelerated apoptosis of LUSC cells. Additionally, we observed the increased expression of HMGB3 and its positive correlation with PITPNA-AS1 in LUSC samples. Interestingly, PITPNA-AS1 mainly located in the cytosol of LUSC cells, and also affected mRNA stability of HMGB3. Furthermore, the repressed mRNA stability of HMGB3 by PITPNA-AS1 via TAF15 was exposed through mechanism experiments. The mediatory function of PITPNA-AS1 on HMGB3 was validated via rescue assays. All in all, PITPNA-AS1 promoted the proliferation and migration of LUSC cells via stabilizing HMGB3 by TAF15. In conclusion, our study displayed a novel mechanism underlying PITPNA-AS1 in LUSC cells.", "gemini": "Nhiều báo cáo đã thăm dò sự tham gia của các lncRNA được biểu hiện bất thường trong nhiều loại ung thư, bao gồm cả ung thư biểu mô tế bào vảy phổi (LUSC). Thông qua cơ sở dữ liệu trực tuyến GEPIA, lncRNA PITPNA antisense RNA 1 (PITPNA-AS1) được biểu hiện cao trong các mẫu LUSC và xu hướng này được khẳng định thêm ở các tế bào LUSC. Mục đích của nghiên cứu hiện tại là điều tra cơ chế liên quan của PITPNA-AS1 trong LUSC. Các thí nghiệm chức năng đã xác minh rằng sự suy giảm PITPNA-AS1 cản trở khả năng tăng sinh và di chuyển, nhưng lại đẩy nhanh quá trình apoptosis của các tế bào LUSC. Ngoài ra, chúng tôi quan sát thấy sự gia tăng biểu hiện của HMGB3 và mối tương quan tích cực của nó với PITPNA-AS1 trong các mẫu LUSC. Điều thú vị là PITPNA-AS1 chủ yếu nằm trong tế bào chất của các tế bào LUSC và cũng ảnh hưởng đến độ ổn định mRNA của HMGB3. Hơn nữa, độ ổn định mRNA bị kìm hãm của HMGB3 do PITPNA-AS1 thông qua TAF15 đã được phát hiện thông qua các thí nghiệm cơ chế. Chức năng trung gian của PITPNA-AS1 trên HMGB3 đã được xác nhận thông qua các xét nghiệm cứu hộ. Nhìn chung, PITPNA-AS1 thúc đẩy sự phát triển và di chuyển của các tế bào LUSC thông qua việc ổn định HMGB3 bởi TAF15. Tóm lại, nghiên cứu của chúng tôi đã chỉ ra một cơ chế mới cơ bản của PITPNA-AS1 trong các tế bào LUSC."} +{"idx": 1871073, "en": "BACKGROUND There is limited information about HRQL after pediatric heart transplantation at a young age. METHODS Prospective follow-up study of children who received a heart transplant at age ≤4 years. HRQL was assessed using the PedsQLTM 4.0 at age 4.5 years. This cohort was compared with healthy children, children with CHD, and with chronic conditions. Peri-operative factors associated with HRQL were also explored. RESULTS Of 66 eligible patients, 15 (23%) died prior to the HRQL assessment and 2 (3%) were lost to follow-up, leaving 49 patients. Indication for transplantation was CHD in 27 (55%) and CMP in 22 (45%). Median age (IQR) at transplant was 9 (5-31) months. HRQL was significantly lower in transplanted children compared to population norms (65.3 vs 87.3, P < .0001), children with chronic conditions (65.3 vs 76.1, P = .001), and children with CHD (65.3 vs 81.1, P < .0001). Transplanted children with CHD had lower HRQL than those with a prior diagnosis of CMP (59.5 vs 72.5, P-value = .020). Higher creatinine pretransplant and higher lactate post-operatively were associated with lower HRQL. CONCLUSION Children after heart transplant had significantly lower HRQL, as reported by their parents, than the normative population, children with chronic conditions, and children with CHD.", "gemini": "BỐI CẢNH Có ít thông tin về HRQL sau khi ghép tim ở trẻ em khi còn nhỏ. PHƯƠNG PHÁP Nghiên cứu theo dõi tiến triển của trẻ em được ghép tim khi ≤4 tuổi. HRQL được đánh giá bằng PedsQLTM 4.0 khi trẻ 4,5 tuổi. Nhóm bệnh nhân này được so sánh với trẻ em khỏe mạnh, trẻ em mắc bệnh tim bẩm sinh (CHD) và trẻ em mắc các bệnh mãn tính. Các yếu tố quanh phẫu thuật liên quan đến HRQL cũng được khám phá. KẾT QUẢ Trong số 66 bệnh nhân đủ điều kiện, 15 (23%) đã tử vong trước khi đánh giá HRQL và 2 (3%) bị mất theo dõi, còn lại 49 bệnh nhân. Chỉ định ghép tim là CHD ở 27 (55%) và CMP ở 22 (45%). Độ tuổi trung bình (IQR) khi ghép là 9 (5-31) tháng. HRQL thấp hơn đáng kể ở trẻ em được ghép so với các chuẩn mực của dân số (65,3 so với 87,3, P <0,0001), trẻ em mắc bệnh mãn tính (65,3 so với 76,1, P = 0,001) và trẻ em mắc CHD (65,3 so với 81,1, P <0,0001). Trẻ em được ghép tim mắc CHD có HRQL thấp hơn so với trẻ được chẩn đoán trước đó là CMP (59,5 so với 72,5, P = 0,020). Creatinin cao hơn trước khi ghép và lactate cao hơn sau phẫu thuật có liên quan đến HRQL thấp hơn. KẾT LUẬN Trẻ em sau khi ghép tim có HRQL thấp hơn đáng kể, theo báo cáo của cha mẹ chúng, so với dân số chuẩn mực, trẻ em mắc bệnh mãn tính và trẻ em mắc CHD."} +{"idx": 1871074, "en": "In this work, we joined highly Ni-loaded γ-Al2 O3 composites, straightforwardly prepared by impregnation methods, with an induction heating setup suited to control, almost in real-time, any temperature swing at the catalyst sites (i. e., \"hot spots\" ignition) caused by an exothermic reaction at the heart of the power-to-gas (P2G) chain: CO2 methanation. We have shown how the combination of a poor thermal conductor (γ-Al2 O3 ) as support for large and highly interconnected nickel aggregates together with a fast heat control of the temperature at the catalytic bed allow part of the extra-heat generated by the reaction exothermicity to be reused for maintaining the catalyst under virtual isothermal conditions, hence reducing the reactor power supply. Most importantly, a highly efficient methanation scheme for substitute natural gas (SNG) production (X up 98 % with >99 % S ) under operative temperatures (150-230 °C) much lower than those commonly required with traditional heating setup has been proposed. As far as sustainable and environmental issues are concerned, this approach re-evaluates industrially attractive composites (and their large-scale preparation methods) for application to key processes at the heart of P2G chain while providing robust catalysts for which risks associated to nano-objects leaching phenomena are markedly reduced if not definitively suppressed.", "gemini": "Trong công trình này, chúng tôi đã kết hợp các hợp chất γ-Al2 O3 có tải Ni cao, được chế tạo trực tiếp bằng phương pháp tẩm, với hệ thống gia nhiệt cảm ứng phù hợp để kiểm soát, gần như theo thời gian thực, bất kỳ sự thay đổi nhiệt độ nào tại các vị trí xúc tác (tức là quá trình đánh lửa \"điểm nóng\") do phản ứng tỏa nhiệt ở trung tâm của chuỗi năng lượng thành khí (P2G): phản ứng metan hóa CO2. Chúng tôi đã chỉ ra cách kết hợp một chất dẫn nhiệt kém (γ-Al2 O3 ) làm chất hỗ trợ cho các tập hợp niken lớn và có sự kết nối chặt chẽ với nhau cùng với khả năng kiểm soát nhiệt độ nhanh chóng tại lớp xúc tác cho phép một phần nhiệt lượng tỏa ra do phản ứng tỏa nhiệt được tái sử dụng để duy trì chất xúc tác trong điều kiện gần như đẳng nhiệt, do đó giảm nguồn cung cấp năng lượng cho lò phản ứng. Quan trọng nhất, một sơ đồ metan hóa hiệu suất cao để sản xuất khí tự nhiên thay thế (SNG) (X lên đến 98% với S > 99%) ở nhiệt độ hoạt động (150-230°C) thấp hơn nhiều so với nhiệt độ thông thường. yêu cầu với thiết lập sưởi ấm truyền thống đã được đề xuất. Trong phạm vi các vấn đề bền vững và môi trường, cách tiếp cận này đánh giá lại các hợp chất hấp dẫn về mặt công nghiệp (và các phương pháp chế tạo quy mô lớn của chúng) để ứng dụng vào các quy trình chính tại trung tâm của chuỗi P2G đồng thời cung cấp các chất xúc tác mạnh mẽ mà rủi ro liên quan đến hiện tượng rửa trôi nano-vật thể được giảm đáng kể nếu không bị triệt tiêu hoàn toàn."} +{"idx": 1871075, "en": "Despite correction of underlying solid organ failure by transplantation, pediatric transplant recipients still have increased mortality rates compared to the general pediatric population, in part due to increased cardiovascular risk. In particular, pediatric kidney and non-kidney transplant recipients with chronic kidney disease have significant cardiovascular risk that worsens with declining kidney function. Biomarkers associated with future cardiovascular risk such as casual and ambulatory hypertension, dyslipidemia, vascular stiffness and calcification, and left ventricular hypertrophy can be detected throughout the post-transplant period and in patients with stable kidney function. Among these, hypertension and dyslipidemia are two potentially modifiable cardiovascular risk factors that are highly prevalent in kidney and non-kidney pediatric transplant recipients. Standardized approaches to appropriate BP measurement and lipid monitoring are needed to detect and address these risk factors in a timely fashion. To achieve sustained improvement in cardiovascular health, clinicians should partner with patients and their caregivers to address these and other risk factors with a combined approach that integrates pharmacologic with non-pharmacologic approaches. This review outlines the scope and impact of hypertension and dyslipidemia in pediatric transplant recipients, with a particular focus on pediatric kidney transplantation given the high burden of chronic kidney disease-associated cardiovascular risk. We also review the current published guidelines for monitoring and managing abnormalities in blood pressure and lipids, highlighting the important role of therapeutic lifestyle changes in concert with antihypertensive and lipid-lowering medications.", "gemini": "Mặc dù đã điều chỉnh được tình trạng suy tạng rắn tiềm ẩn bằng phương pháp ghép tạng, nhưng tỷ lệ tử vong của người nhận ghép tạng nhi vẫn cao hơn so với dân số nhi khoa nói chung, một phần là do nguy cơ tim mạch tăng cao. Đặc biệt, những người nhận ghép thận và ghép tạng khác ngoài thận ở trẻ em mắc bệnh thận mãn tính có nguy cơ tim mạch đáng kể, tình trạng này trở nên tồi tệ hơn khi chức năng thận suy giảm. Các dấu ấn sinh học liên quan đến nguy cơ tim mạch trong tương lai như tăng huyết áp thông thường và khi đi lại, rối loạn lipid máu, cứng và vôi hóa mạch máu, và phì đại thất trái có thể được phát hiện trong suốt thời gian sau ghép và ở những bệnh nhân có chức năng thận ổn định. Trong số các yếu tố này, tăng huyết áp và rối loạn lipid máu là hai yếu tố nguy cơ tim mạch có thể thay đổi được và rất phổ biến ở những người nhận ghép thận và ghép tạng khác ngoài thận ở trẻ em. Cần có các phương pháp tiếp cận chuẩn hóa để đo huyết áp và theo dõi lipid thích hợp để phát hiện và giải quyết các yếu tố nguy cơ này kịp thời. Để đạt được sự cải thiện bền vững về sức khỏe tim mạch, các bác sĩ lâm sàng nên hợp tác với bệnh nhân và người chăm sóc của họ để giải quyết các yếu tố nguy cơ này và các yếu tố nguy cơ khác bằng cách tiếp cận kết hợp giữa các phương pháp dược lý và phi dược lý. Bản đánh giá này phác thảo phạm vi và tác động của tăng huyết áp và rối loạn lipid máu ở những người nhận ghép tạng nhi, đặc biệt tập trung vào ghép thận ở trẻ em do gánh nặng cao của nguy cơ tim mạch liên quan đến bệnh thận mãn tính. Chúng tôi cũng xem xét các hướng dẫn được công bố hiện tại để theo dõi và quản lý các bất thường về huyết áp và lipid, nêu bật vai trò quan trọng của những thay đổi lối sống điều trị kết hợp với thuốc hạ huyết áp và thuốc hạ lipid."} +{"idx": 1871076, "en": "BACKGROUND Craniofacial microsomia (CFM), also known as the oculo-auriculo-vertebral spectrum, comprises a variable phenotype with the most common features including microtia and mandibular hypoplasia on one or both sides, in addition to lateral oral clefts, epibulbar dermoids, cardiac, vertebral, and renal abnormalities. The etiology of CFM is largely unknown. The MYT1 gene has been reported as a candidate based in mutations found in three unrelated individuals. Additional patients with mutations in this gene are required to establish its causality. We present two individuals with CFM that have rare variants in MYT1 contributing to better understand the genotype and phenotype associated with mutations in this gene. METHODS/RESULTS We conducted genetic analysis using whole-exome and -genome sequencing in 128 trios with CFM. Two novel MYT1 mutations were identified in two participants. Sanger sequencing was used to confirm these mutations. CONCLUSION We identified two additional individuals with CFM who carry rare variants in MYT1, further supporting the presumptive role of this gene in the CFM spectrum.", "gemini": "LÝ LỊCH Vi thể não mặt (CFM), còn được gọi là quang phổ mắt-tai-đốt sống, bao gồm một kiểu hình thay đổi với các đặc điểm phổ biến nhất bao gồm vi tai và hạ sản xương hàm ở một hoặc cả hai bên, ngoài các khe hở bên miệng, da mắt, tim, đốt sống, và bất thường về thận. Nguyên nhân của CFM phần lớn vẫn chưa được biết. Gen MYT1 đã được báo cáo là một ứng cử viên dựa trên các đột biến được tìm thấy ở ba cá thể không có quan hệ họ hàng. Cần có thêm những bệnh nhân có đột biến ở gen này để xác lập tính nhân quả của nó. Chúng tôi trình bày hai cá thể mắc CFM có các biến thể hiếm ở MYT1 góp phần hiểu rõ hơn về kiểu gen và kiểu hình liên quan đến các đột biến ở gen này. PHƯƠNG PHÁP / KẾT QUẢ Chúng tôi đã tiến hành phân tích di truyền bằng cách giải trình tự toàn bộ exome và toàn bộ hệ gen ở 128 bộ ba có CFM. Hai đột biến MYT1 mới đã được xác định ở hai người tham gia. Giải trình tự Sanger đã được sử dụng để xác nhận các đột biến này. KẾT LUẬN Chúng tôi đã xác định được hai cá thể khác mắc CFM mang các biến thể hiếm ở MYT1, tiếp tục hỗ trợ vai trò phỏng đoán của gen này trong quang phổ CFM."} +{"idx": 1871077, "en": "Facilitating the charge separation of semiconductor photocatalysts to increase the photocatalytic CO2 reduction activity has become a great challenge for sustainable energy conversion. Herein, the surface halogen-modified defect-rich Bi2 WO6 nanosheets have been successfully prepared to address the aforementioned challenge. Importantly, the modification of surface with halogen atoms is beneficial for the adsorption and activation for CO2 molecules and charge separation. These properties have been analyzed by experimental and theoretical methods. DFT calculations revealed that the modification of the Bi2 WO6 surface with Br atoms can decrease the formation energy of the *COOH intermediate, which accelerates CO2 conversion. All halogen-modified defect-rich Bi2 WO6 nanosheets showed an enhanced photocatalytic CO2 reduction activity. Specifically, Br-Bi2 WO6 exhibited the best CO generation rate of 13.8 μmol g-1 h-1 , which is roughly 7.3 times as high as the unmodified defect-rich Bi2 WO6 (1.9 μmol g-1 h-1 ). Moreover, in the presence of a cocatalyst (cobalt phthalocyanine) and a sacrificial agent (triethanolamine), Br-Bi2 WO6 exhibited an even further improved CO generation rate of 187 μmol g-1  h-1 . This finding provides a new approach to optimize the CO2 reduction pathway of semiconductor photocatalysts, which is beneficial to develop highly efficient CO2 reduction photocatalysts.", "gemini": "Việc tạo điều kiện tách điện tích của các chất xúc tác quang bán dẫn để tăng hoạt động quang xúc tác khử CO2 đã trở thành một thách thức lớn đối với quá trình chuyển đổi năng lượng bền vững. Trong bài viết này, các tấm nano Bi2 WO6 giàu khuyết tật đã được sửa đổi bằng halogen trên bề mặt để giải quyết thách thức nói trên. Điều quan trọng là việc sửa đổi bề mặt bằng các nguyên tử halogen có lợi cho quá trình hấp phụ và hoạt hóa các phân tử CO2 cũng như tách điện tích. Các tính chất này đã được phân tích bằng các phương pháp thực nghiệm và lý thuyết. Các tính toán DFT cho thấy việc sửa đổi bề mặt Bi2 WO6 bằng các nguyên tử Br có thể làm giảm năng lượng tạo thành của chất trung gian *COOH, giúp đẩy nhanh quá trình chuyển đổi CO2. Tất cả các tấm nano Bi2 WO6 giàu khuyết tật đã được sửa đổi bằng halogen đều cho thấy hoạt động quang xúc tác khử CO2 được cải thiện. Cụ thể, Br-Bi2 WO6 thể hiện tốc độ tạo ra CO tốt nhất là 13,8 μmol g-1 h-1, cao hơn khoảng 7,3 lần so với Bi2 WO6 giàu khuyết tật chưa được sửa đổi (1,9 μmol g-1 h-1). Hơn nữa, khi có mặt của chất xúc tác đồng (coban phthalocyanine) và chất hy sinh (triethanolamine), Br-Bi2 WO6 cho thấy tốc độ tạo ra CO được cải thiện hơn nữa là 187 μmol g-1 h-1. Phát hiện này cung cấp một cách tiếp cận mới để tối ưu hóa con đường khử CO2 của các chất xúc tác quang bán dẫn, có lợi cho việc phát triển các chất xúc tác quang khử CO2 có hiệu suất cao."} +{"idx": 1871078, "en": "The dried stem bark of Berberis kansuensis C.K.Schneid. (Berberidaceae) was widely used to treat diabetes in traditional Tibetan medicine system. However, its anti-diabetic mechanisms have not been elucidated. In this study, 1 H-NMR-based metabolomics combined with biochemistry assay was applied to investigate the anti-diabetic activities as well as underlying mechanisms of B. kansuensis extract on type 2 diabetic rats. The results showed that after 30 days treatment with B. kansuensis extract, the levels of FBG, GSP, INS, TNF-α, IL-1β and IL-6 were significantly decreased in B. kansuensis group compared with the model group. Besides, a total of 28 metabolites were identified in rat serum by 1 H-NMR-based metabolomics method, 16 of which were significantly different in the normal group compared with the model group, and eight of them were significantly reversed after B. kansuensis intervention. Further analysis of metabolic pathways indicated that therapeutic effect of B. kansuensis might be predominantly related to their ability to improve glycolysis and gluconeogenesis, citric acid cycle, lipid metabolism, amino acid metabolism and choline metabolism. The results of both metabolomics and biochemical analysis indicated that B. kansuensis extract has a potential anti-diabetic effect on type 2 diabetic rats. Its therapeutic effect may be based on the ability of anti-inflammation, alleviating insulin resistance and restoring several disturbed metabolic pathways.", "gemini": "Vỏ thân cây khô của Berberis kansuensis C.K.Schneid. (Berberidaceae) được sử dụng rộng rãi để điều trị bệnh tiểu đường trong hệ thống y học Tây Tạng truyền thống. Tuy nhiên, cơ chế chống đái tháo đường của nó vẫn chưa được làm sáng tỏ. Trong nghiên cứu này, phương pháp chuyển hóa dựa trên 1 H-NMR kết hợp với xét nghiệm sinh hóa đã được áp dụng để nghiên cứu các hoạt động chống đái tháo đường cũng như cơ chế cơ bản của chiết xuất B. kansuensis trên chuột mắc bệnh tiểu đường loại 2. Kết quả cho thấy sau 30 ngày điều trị bằng chiết xuất B. kansuensis, nồng độ FBG, GSP, INS, TNF-α, IL-1β và IL-6 giảm đáng kể ở nhóm B. kansuensis so với nhóm mô hình. Bên cạnh đó, tổng cộng 28 chất chuyển hóa đã được xác định trong huyết thanh chuột bằng phương pháp chuyển hóa dựa trên 1 H-NMR, 16 chất trong số đó có sự khác biệt đáng kể ở nhóm bình thường so với nhóm mô hình và tám chất trong số đó được đảo ngược đáng kể sau khi can thiệp B. kansuensis. Phân tích sâu hơn về các con đường chuyển hóa chỉ ra rằng tác dụng điều trị của B. kansuensis có thể chủ yếu liên quan đến khả năng cải thiện quá trình đường phân và tân tạo đường, chu trình axit citric, chuyển hóa lipid, chuyển hóa axit amin và chuyển hóa choline. Kết quả của cả phân tích chuyển hóa và phân tích sinh hóa cho thấy chiết xuất B. kansuensis có tác dụng chống đái tháo đường tiềm tàng trên chuột mắc bệnh tiểu đường loại 2. Tác dụng điều trị của nó có thể dựa trên khả năng chống viêm, giảm tình trạng kháng insulin và phục hồi một số con đường chuyển hóa bị rối loạn."} +{"idx": 1871079, "en": "As an indispensable, even lifesaving practice, red blood cell (RBC) transfusion is challenging due to several issues, including supply shortage, immune incompatibility, and blood-borne infections since donated blood is the only source of RBCs. Although large-scale in vitro production of functional RBCs from human stem cells is a promising alternative, so far, no such system has been reported to produce clinically transfusable RBCs due to the poor understanding of mechanisms of human erythropoiesis, which is essential for the optimization of in vitro erythrocyte generation system. We previously reported that inhibition of mammalian target of rapamycin (mTOR) signaling significantly decreased the percentage of erythroid progenitor cells in the bone marrow of wild-type mice. In contrast, rapamycin treatment remarkably improved terminal maturation of erythroblasts and anemia in a mouse model of β-thalassemia. In the present study, we investigated the effect of mTOR inhibition with rapamycin from different time points on human umbilical cord blood-derived CD34+ cell erythropoiesis in vitro and the underlying mechanisms. Our data showed that rapamycin treatment significantly suppressed erythroid colony formation in the commitment/proliferation phase of erythropoiesis through inhibition of cell-cycle progression and proliferation. In contrast, during the maturation phase of erythropoiesis, mTOR inhibition dramatically promoted enucleation and mitochondrial clearance by enhancing autophagy. Collectively, our results suggest contrasting roles for mTOR in regulating different phases of human erythropoiesis.", "gemini": "Là một phương pháp thực hành không thể thiếu, thậm chí là cứu sống, truyền hồng cầu (RBC) đang phải đối mặt với nhiều thách thức do một số vấn đề, bao gồm tình trạng thiếu hụt nguồn cung, không tương thích về miễn dịch và nhiễm trùng do máu vì máu được hiến là nguồn cung cấp RBC duy nhất. Mặc dù việc sản xuất hồng cầu chức năng trong ống nghiệm trên quy mô lớn từ tế bào gốc của con người là một phương pháp thay thế đầy hứa hẹn, cho đến nay, vẫn chưa có hệ thống nào như vậy được báo cáo là có thể sản xuất ra hồng cầu có thể truyền được trên lâm sàng do hiểu biết kém về cơ chế tạo hồng cầu ở người, điều này rất cần thiết cho việc tối ưu hóa hệ thống tạo hồng cầu trong ống nghiệm. Trước đây, chúng tôi đã báo cáo rằng việc ức chế tín hiệu mục tiêu của động vật có vú đối với rapamycin (mTOR) làm giảm đáng kể tỷ lệ tế bào tiền thân tạo hồng cầu trong tủy xương của chuột hoang dã. Ngược lại, việc điều trị bằng rapamycin cải thiện đáng kể sự trưởng thành cuối cùng của các nguyên hồng cầu và tình trạng thiếu máu ở mô hình chuột bị bệnh tan máu β. Trong nghiên cứu hiện tại, chúng tôi đã nghiên cứu tác dụng của việc ức chế mTOR bằng rapamycin từ các thời điểm khác nhau đối với quá trình tạo hồng cầu từ tế bào CD34+ có nguồn gốc từ máu dây rốn người trong ống nghiệm và các cơ chế cơ bản. Dữ liệu của chúng tôi cho thấy việc điều trị bằng rapamycin ức chế đáng kể sự hình thành khuẩn lạc tạo hồng cầu trong giai đoạn cam kết/phân bào của quá trình tạo hồng cầu thông qua việc ức chế sự tiến triển của chu kỳ tế bào và sự tăng sinh. Ngược lại, trong giai đoạn trưởng thành của quá trình tạo hồng cầu, việc ức chế mTOR thúc đẩy đáng kể quá trình loại bỏ nhân và thanh thải ty thể bằng cách tăng cường quá trình tự thực. Nhìn chung, kết quả của chúng tôi cho thấy vai trò trái ngược nhau của mTOR trong việc điều chỉnh các giai đoạn khác nhau của quá trình tạo hồng cầu ở người."} +{"idx": 1871080, "en": "Covalent organic frameworks (COFs) are relatively recent materials. They have received great attention due to their interesting properties. However, the application of microwaves in their synthesis, despite its advantages such as faster and more reproducible processes, is a minority. Herein, a comprehensive compilation of the research results published in the microwave-assisted synthesis (MAS) of COFs is presented. This review includes articles of 2D and 3D COFs prepared using microwaves as source of energy. The articles have been classified depending on the functional groups including boronate ester, imines, enamines, azines, and triazines, among others. It compiles the main parameters of synthesis and characteristics of the materials together with some general issues related with COFs and microwaves. Additionally, current and future perspectives of the topic have been discussed.", "gemini": "Khung hữu cơ cộng hóa trị (COF) là vật liệu tương đối mới. Chúng nhận được sự chú ý lớn do các tính chất thú vị của chúng. Tuy nhiên, việc ứng dụng vi sóng trong quá trình tổng hợp của chúng, mặc dù có những ưu điểm như quy trình nhanh hơn và có thể tái tạo được nhiều hơn, nhưng lại là thiểu số. Tại đây, chúng tôi trình bày một biên soạn toàn diện về các kết quả nghiên cứu được công bố trong quá trình tổng hợp COF hỗ trợ bằng vi sóng (MAS). Đánh giá này bao gồm các bài báo về COF 2D và 3D được chế tạo bằng vi sóng làm nguồn năng lượng. Các bài báo đã được phân loại tùy thuộc vào các nhóm chức năng bao gồm este boronat, imin, enamin, azin và triazin, trong số những nhóm khác. Nó biên soạn các thông số chính của quá trình tổng hợp và đặc điểm của vật liệu cùng với một số vấn đề chung liên quan đến COF và vi sóng. Ngoài ra, các góc nhìn hiện tại và tương lai của chủ đề đã được thảo luận."} +{"idx": 1871081, "en": "OBJECTIVES We aimed to reconstitute T2 and T3 stage classification in nasopharyngeal carcinoma (NPC) cases and verify its utility in clinical settings. MATERIALS AND METHODS We enrolled 792 NPC patients. Cox proportional hazards model was used to compare the effect sizes (hazard ratio [HR]) of the cranial structure invasion on survival and select the structures for up-staging or downstaging T2 and T3 NPC. The samples were reclassified and the survival curves for T2 and T3 stages were analyzed. The proposed new staging system was validated on an external sample (n = 433). RESULTS Thirteen cranial structures were examined. American Joint Committee on Cancer (AJCC) T3 stage patients with the invasion of the base of the sphenoid (HR = 2.58, 95% CI = 1.16-5.77) or base of the pterygoid (HR = 2.00, 95% CI = 0.84-4.77) had significantly lower hazard ratios than T2 stage patients with the invasion of soft tissues in the bilateral parapharyngeal space (HR = 5.26, 95% CI = 2.02-13.68) and single/bilateral carotid sheath (HR = 7.78, 95% CI = 3.06-19.76). T3 stage with the invasion of the above-mentioned bones was reclassified as T2, and T2 stage with the invasion of the above-mentioned soft-tissue structures was reclassified as T3. Survival analysis showed a significant difference between the reclassified T2 and T3 stages (P < 0.001). The results were replicated in the validation samples. CONCLUSION The proposed staging system for defining T2 and T3 stage NPC appears to be superior to the AJCC 8th edition. It could improve prognosis and optimize the treatment selection.", "gemini": "MỤC TIÊU Chúng tôi nhằm mục đích tái tạo phân loại giai đoạn T2 và T3 trong các trường hợp ung thư vòm họng (NPC) và xác minh tính hữu ích của nó trong các thiết lập lâm sàng. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi đã đăng ký 792 bệnh nhân NPC. Mô hình nguy cơ tỷ lệ Cox được sử dụng để so sánh kích thước hiệu ứng (tỷ lệ nguy cơ [HR]) của sự xâm lấn cấu trúc sọ đối với sự sống còn và chọn các cấu trúc để nâng giai đoạn hoặc hạ giai đoạn NPC T2 và T3. Các mẫu đã được phân loại lại và các đường cong sống còn cho giai đoạn T2 và T3 đã được phân tích. Hệ thống phân giai đoạn mới được đề xuất đã được xác nhận trên một mẫu bên ngoài (n = 433). KẾT QUẢ Mười ba cấu trúc sọ đã được kiểm tra. Bệnh nhân giai đoạn T3 của Ủy ban Ung thư Hoa Kỳ (AJCC) có sự xâm lấn của đáy xương bướm (HR = 2,58, KTCB 95% = 1,16-5,77) hoặc đáy xương cánh bướm (HR = 2,00, KTCB 95% = 0,84-4,77) có tỷ lệ nguy cơ thấp hơn đáng kể so với bệnh nhân giai đoạn T2 có sự xâm lấn của mô mềm ở khoang hầu hai bên (HR = 5,26, KTCB 95% = 2,02-13,68) và bao cảnh động mạch đơn/hai bên (HR = 7,78, KTCB 95% = 3,06-19,76). Giai đoạn T3 có sự xâm lấn của các xương đã đề cập ở trên được phân loại lại thành T2 và giai đoạn T2 có sự xâm lấn của các cấu trúc mô mềm đã đề cập ở trên được phân loại lại thành T3. Phân tích sống còn cho thấy sự khác biệt đáng kể giữa các giai đoạn T2 và T3 được phân loại lại (P < 0,001). Kết quả đã được sao chép trong các mẫu xác nhận. KẾT LUẬN Hệ thống phân giai đoạn được đề xuất để xác định NPC giai đoạn T2 và T3 có vẻ vượt trội hơn so với AJCC phiên bản thứ 8. Nó có thể cải thiện tiên lượng và tối ưu hóa việc lựa chọn phương pháp điều trị."} +{"idx": 1871082, "en": "BACKGROUND The reconstruction of the anterior cruciate ligament (ACL) of the knee joint is the gold standard in complete ACL rupture treatment. One of the central discussion topics is choice of graft. OBJECTIVES To assess the outcome of treatment after primary ACL reconstruction using allograft. MATERIAL AND METHODS The study was a retrospective cohort study. Out of 372 male patients who had undergone primary unilateral intra-articular ACL reconstruction using strict inclusion and exclusion criteria, 61 patients who qualified for the study took part. Group I was made up of 31 patients with allograft, while Group II consisted of 30 patients involved with autograft. The Lachman test, Pivot-Shift test, Lysholm Knee Scoring scale, and 2000 International Knee Documentation Committee (IKDC) were used to evaluate the results. Follow-up time was 18 months. RESULTS The knee joint regained anterior stability in subjective and objective assessments in all the patients in both groups. The subjective results were the following: in Group I, 96.6 ±3.08 points on the Lysholm scale and 94.79 ±6.53 points on the IKDC 2000 scale, while in Group II, 98.00 ±1.9 points on the Lysholm scale and 94.81 ±5.6 points on the IKDC 2000 scale. The group comparison of the results of the IKDC 2000 and Lysholm Scale obtained postoperatively showed no statistically significant differences between the 2 groups. CONCLUSIONS Primary ACL reconstruction using an allograft is an effective procedure to counteract instability of the anterior knee joint. Comparative analysis of the results of primary ACL reconstruction in the treatment of anterior knee instability using autograft or allograft gives grounds for the possibility of individual selection of graft depending on what the patient's expectations are.", "gemini": "LÝ LỊCH Tái tạo dây chằng chéo trước (ACL) của khớp gối là tiêu chuẩn vàng trong điều trị đứt ACL hoàn toàn. Một trong những chủ đề thảo luận trung tâm là lựa chọn ghép. MỤC TIÊU Đánh giá kết quả điều trị sau khi tái tạo ACL ban đầu bằng ghép dị thể. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu là nghiên cứu theo dõi hồi cứu. Trong số 372 bệnh nhân nam đã trải qua tái tạo ACL nội khớp đơn phương ban đầu bằng các tiêu chuẩn loại trừ và bao gồm nghiêm ngặt, 61 bệnh nhân đủ điều kiện tham gia nghiên cứu. Nhóm I bao gồm 31 bệnh nhân ghép dị thể, trong khi Nhóm II bao gồm 30 bệnh nhân ghép tự thân. Kiểm tra Lachman, kiểm tra Pivot-Shift, thang điểm Lysholm Knee và Ủy ban Tài liệu Đầu gối Quốc tế (IKDC) năm 2000 được sử dụng để đánh giá kết quả. Thời gian theo dõi là 18 tháng. KẾT QUẢ Khớp gối lấy lại được sự ổn định trước trong các đánh giá chủ quan và khách quan ở tất cả bệnh nhân ở cả hai nhóm. Kết quả chủ quan như sau: ở Nhóm I, 96,6 ± 3,08 điểm trên thang điểm Lysholm và 94,79 ± 6,53 điểm trên thang điểm IKDC 2000, trong khi ở Nhóm II, 98,00 ± 1,9 điểm trên thang điểm Lysholm và 94,81 ± 5,6 điểm trên thang điểm IKDC 2000. So sánh nhóm kết quả IKDC 2000 và Thang điểm Lysholm thu được sau phẫu thuật không cho thấy sự khác biệt có ý nghĩa thống kê giữa 2 nhóm. KẾT LUẬN Tái tạo ACL ban đầu bằng ghép dị thể là một thủ thuật hiệu quả để chống lại sự mất ổn định của khớp gối trước. Phân tích so sánh kết quả tái tạo ACL ban đầu trong điều trị mất ổn định gối trước bằng ghép tự thân hoặc ghép dị thể tạo cơ sở cho khả năng lựa chọn ghép riêng tùy thuộc vào kỳ vọng của bệnh nhân."} +{"idx": 1871083, "en": "BACKGROUND Weaning failure is common in mechanically ventilated patients, and if ultrasound can predict weaning outcome remains controversial. The purpose of this study was to evaluate the diaphragmatic function (thickness and excursion) measured by ultrasound as a predictor of the extubation outcome. METHODS We included 62 mechanically ventilated patients from the chest intensive care unit in this study. Sixty-two patients who successfully passed the spontaneous breathing trial (SBT) were enrolled. The transthoracic ultrasound of the diaphragm was performed during an SBT to the assess diaphragmatic function (excursion and thickness), and they were classified into the successful extubation group and the failed extubation group. RESULTS There was a statistically significant increase in the successful extubation group in the diaphragmatic excursion and thickness fraction (p<0.001), a statistically significant negative correlation between the diaphragmatic function and the duration of the mechanical ventilation, and a statistically significant negative correlation between the diaphragmatic excursion and the Acute Physiology and Chronic Health Evaluation II. The diaphragmatic excursion cutoff value predictive of weaning was 1.25 cm, with a specificity of 82.1% and a sensitivity of 97.1% respectively, and the diaphragmatic thickness cut-off value predictive of weaning was 21.5%, with a specificity of 60.7% and a sensitivity of 91.2%, respectively. CONCLUSION The diaphragmatic ultrasonography was found to be a promising tool for predicting the extubation outcome for mechanically ventilated patients.", "gemini": "LÝ LỊCH SƠ LƯỢC Việc cai máy thở không thành công thường gặp ở những bệnh nhân thở máy, và việc liệu siêu âm có thể dự đoán kết quả cai máy thở hay không vẫn còn gây tranh cãi. Mục đích của nghiên cứu này là đánh giá chức năng cơ hoành (độ dày và độ di chuyển) được đo bằng siêu âm như một yếu tố dự báo kết quả rút nội khí quản. PHƯƠNG PHÁP Chúng tôi đã đưa 62 bệnh nhân thở máy từ khoa hồi sức tích cực lồng ngực vào nghiên cứu này. Sáu mươi hai bệnh nhân đã vượt qua thử nghiệm thở tự phát (SBT) được ghi danh. Siêu âm xuyên thành ngực cơ hoành được thực hiện trong quá trình SBT để đánh giá chức năng cơ hoành (độ di chuyển và độ dày) và chúng được phân loại vào nhóm rút nội khí quản thành công và nhóm rút nội khí quản không thành công. KẾT QUẢ Có sự gia tăng có ý nghĩa thống kê ở nhóm rút nội khí quản thành công về độ di chuyển cơ hoành và độ dày phân số (p < 0,001), có mối tương quan âm có ý nghĩa thống kê giữa chức năng cơ hoành và thời gian thở máy và có mối tương quan âm có ý nghĩa thống kê giữa độ di chuyển cơ hoành và Đánh giá sinh lý cấp tính và sức khỏe mãn tính II. Giá trị ngưỡng độ di chuyển cơ hoành dự đoán cai máy thở là 1,25 cm, với độ đặc hiệu là 82,1% và độ nhạy là 97,1% tương ứng và giá trị ngưỡng độ dày cơ hoành dự đoán cai máy thở là 21,5%, với độ đặc hiệu là 60,7% và độ nhạy là 91,2%, tương ứng. KẾT LUẬN Siêu âm cơ hoành được phát hiện là một công cụ đầy hứa hẹn để dự đoán kết quả rút nội khí quản cho bệnh nhân thở máy."} +{"idx": 1871084, "en": "Shape deformable hydrogels have drawn great attention due to their wide applications as soft actuators. Here we report a novel kind of mechanically strong, tough, and shape deformable poly(acrylamide-co-vinylimidazole) [poly(AAm-co-VI)] hydrogel prepared by photoinitiated copolymerization and the followed immersing in a Cu2+ aqueous solution. Strong Cu2+ complexation with imidazole groups dramatically enhances the mechanical properties of the hydrogels, whose tensile strength, elastic modulus, toughness, and fracture energy reach up to 7.7 ± 0.76 MPa, 15.4 ± 1.2 MPa, 23.2 ± 2.5 MJ m-3, and 22.1 ± 2.3 kJ m-2, respectively. More impressively, shape deformation (bending) can be easily achieved by coating Cu2+ solution on one side of hydrogel strips. Furthermore, precise control of the shape deformation from 1D to 2D and 2D to 3D can be achieved by adjusting Cu2+ concentration, coating time, region, and one or two side(s) of hydrogel samples. The Cu2+ complexation provides a simple way to simultaneously improve the mechanical properties of hydrogels and enable them with shape deformability. The mechanically strong, tough, and shape deformable hydrogels might be a promising candidate for soft actuators.", "gemini": "Các loại hydrogel biến dạng hình dạng đã thu hút sự chú ý lớn do ứng dụng rộng rãi của chúng như các bộ truyền động mềm. Ở đây chúng tôi báo cáo một loại hydrogel poly(acrylamide-co-vinylimidazole) [poly(AAm-co-VI)] mới có độ bền cơ học cao, dẻo dai và có thể biến dạng hình dạng được chế tạo bằng cách đồng trùng hợp khởi đầu bằng quang và sau đó ngâm trong dung dịch Cu2+ trong nước. Sự phức hợp Cu2+ mạnh với các nhóm imidazole làm tăng đáng kể các tính chất cơ học của hydrogel, trong đó độ bền kéo, mô đun đàn hồi, độ dẻo dai và năng lượng gãy đạt tới 7,7 ± 0,76 MPa, 15,4 ± 1,2 MPa, 23,2 ± 2,5 MJ m-3 và 22,1 ± 2,3 kJ m-2, tương ứng. Ấn tượng hơn, có thể dễ dàng đạt được biến dạng hình dạng (uốn cong) bằng cách phủ dung dịch Cu2+ lên một mặt của dải hydrogel. Hơn nữa, có thể kiểm soát chính xác biến dạng hình dạng từ 1D sang 2D và 2D sang 3D bằng cách điều chỉnh nồng độ Cu2+, thời gian phủ, vùng và một hoặc hai mặt của mẫu hydrogel. Sự phức hợp Cu2+ cung cấp một cách đơn giản để đồng thời cải thiện các tính chất cơ học của hydrogel và cho phép chúng có khả năng biến dạng hình dạng. Các loại hydrogel có độ bền cơ học cao, dẻo dai và có thể biến dạng hình dạng có thể là ứng cử viên đầy hứa hẹn cho các bộ truyền động mềm."} +{"idx": 1871085, "en": "3-Acetoacetyl-4,6-diaryl-2-pyridones are synthesized in three steps from chalcones and then condense with carbon disulfide to afford 8-azachromones containing a methylthio group at C2. This leaving group offers an entry point for the insertion of more complex moieties via nucleophilic substitution. For this purpose, N-nucleophiles are explored according to their positions in the Mayr's nucleophilicity scale (N parameter), and three main classes are distinguished depending on whether the substitution takes place from their neutral forms, from their deprotonated anionic forms, or under nucleophilic catalysis. A broad range of primary and secondary amines may be inserted by this method, including enantiomerically pure amino acids, enabling us to explore structural diversity.", "gemini": "3-Acetoacetyl-4,6-diaryl-2-pyridon được tổng hợp theo ba bước từ chalcone và sau đó ngưng tụ với cacbon disulfide để tạo ra 8-azachromon chứa nhóm methylthio tại C2. Nhóm rời này cung cấp một điểm vào để đưa vào các nhóm phức tạp hơn thông qua phản ứng thế ái nhân. Với mục đích này, các N-ái nhân được khám phá theo vị trí của chúng trong thang ái nhân của Mayr (tham số N) và ba lớp chính được phân biệt tùy thuộc vào việc thế diễn ra từ dạng trung tính của chúng, từ dạng anion khử proton của chúng hoặc theo xúc tác ái nhân. Một loạt các amin bậc một và bậc hai có thể được đưa vào bằng phương pháp này, bao gồm các axit amin tinh khiết về mặt đối quang, cho phép chúng ta khám phá sự đa dạng về cấu trúc."} +{"idx": 1871086, "en": "Heterostructures of inorganic halide perovskites with mixed-dimensional inorganic nanomaterials have shown great potential not only in the field of optoelectronic energy devices and photocatalysis but also for improving our fundamental understanding of the charge transfer across the heterostructure interface. Herein, we present for the first time the heterostructure integration of the CsPbBr3 nanocrystal with an N-doped carbon dot. We explore the photoluminescence (PL) and photoconductivity of the heterostructure of CsPbBr3 nanocrystals and N-doped carbon dots. PL quenching of CsPbBr3 nanocrystals with the addition of N-doped carbon dots was observed. The photoexcited electrons from the conduction band of CsPbBr3 are trapped in the N-acceptor state of N-doped carbon dots, and the charge transfer occurs via quasi type II-like electronic band alignment. The charge transfer in the halide perovskite-based heterostructure should motivate further research into the new heterostructure synthesis with perovskites and the fundamental understanding of the mechanism of charge/energy transfer across the heterostructure interface.", "gemini": "Các cấu trúc dị thể của perovskite halide vô cơ với vật liệu nano vô cơ đa chiều đã cho thấy tiềm năng lớn không chỉ trong lĩnh vực thiết bị năng lượng quang điện tử và quang xúc tác mà còn để cải thiện sự hiểu biết cơ bản của chúng ta về sự truyền điện tích qua giao diện cấu trúc dị thể. Trong bài báo này, chúng tôi lần đầu tiên trình bày sự tích hợp cấu trúc dị thể của tinh thể nano CsPbBr3 với một chấm cacbon được pha tạp N. Chúng tôi khám phá quang phát quang (PL) và quang dẫn của cấu trúc dị thể của các tinh thể nano CsPbBr3 và các chấm cacbon được pha tạp N. Đã quan sát thấy sự dập tắt PL của các tinh thể nano CsPbBr3 khi bổ sung các chấm cacbon được pha tạp N. Các electron bị kích thích từ dải dẫn của CsPbBr3 bị giữ lại trong trạng thái chấp nhận N của các chấm cacbon được pha tạp N và sự truyền điện tích xảy ra thông qua sự sắp xếp dải điện tử giống như loại II. Sự truyền điện tích trong cấu trúc dị thể dựa trên perovskite halide sẽ thúc đẩy nghiên cứu sâu hơn về tổng hợp cấu trúc dị thể mới với perovskite và sự hiểu biết cơ bản về cơ chế truyền điện tích/năng lượng qua giao diện cấu trúc dị thể."} +{"idx": 1871087, "en": "The Viral Protein 35 (VP35), a crucial protein of the Zaire Ebolavirus (EBOV), interacts with a plethora of human proteins to cripple the human immune system. Despite its importance, the entire structure of the tetrameric assembly of EBOV VP35 and the means by which it antagonizes the autophosphorylation of the kinase domain of human protein kinase R (PKRK) is still elusive. We consult existing structural information to model a tetrameric assembly of the VP35 protein where 93% of the protein is modeled using crystal structure templates. We analyze our modeled tetrameric structure to identify interchain bonding networks and use molecular dynamics simulations and normal-mode analysis to unravel the flexibility and deformability of the different regions of the VP35 protein. We establish that the C-terminal of VP35 (VP35C) directly interacts with PKRK to prevent it from autophosphorylation. Further, we identify three plausible VP35C-PKRK complexes with better affinity than the PKRK dimer formed during autophosphorylation and use protein design to establish a new stretch in VP35C that interacts with PKRK. The proposed tetrameric assembly will aid in better understanding of the VP35 protein, and the reported VP35C-PKRK complexes along with their interacting sites will help in the shortlisting of small molecule inhibitors.", "gemini": "Protein 35 của vi-rút (VP35), một protein quan trọng của vi-rút Ebola Zaire (EBOV), tương tác với rất nhiều protein của con người để làm tê liệt hệ thống miễn dịch của con người. Mặc dù có tầm quan trọng như vậy, toàn bộ cấu trúc của tập hợp bốn phân tử của VP35 EBOV và phương tiện mà nó đối kháng với quá trình tự phosphoryl hóa của miền kinase của protein kinase R (PKRK) của con người vẫn còn khó nắm bắt. Chúng tôi tham khảo thông tin cấu trúc hiện có để mô hình hóa một tập hợp bốn phân tử của protein VP35, trong đó 93% protein được mô hình hóa bằng cách sử dụng các khuôn mẫu cấu trúc tinh thể. Chúng tôi phân tích cấu trúc bốn phân tử được mô hình hóa của mình để xác định các mạng liên kết giữa các chuỗi và sử dụng mô phỏng động học phân tử và phân tích chế độ bình thường để làm sáng tỏ tính linh hoạt và khả năng biến dạng của các vùng khác nhau của protein VP35. Chúng tôi thiết lập rằng đầu cuối C của VP35 (VP35C) tương tác trực tiếp với PKRK để ngăn chặn quá trình tự phosphoryl hóa của nó. Hơn nữa, chúng tôi xác định ba phức hợp VP35C-PKRK hợp lý có ái lực tốt hơn so với dị hợp PKRK được hình thành trong quá trình tự phosphoryl hóa và sử dụng thiết kế protein để thiết lập một chuỗi mới trong VP35C tương tác với PKRK. Tập hợp bốn phân tử được đề xuất sẽ giúp hiểu rõ hơn về protein VP35 và các phức hợp VP35C-PKRK được báo cáo cùng với các vị trí tương tác của chúng sẽ giúp lập danh sách ngắn các chất ính chế phân tử nhỏ."} +{"idx": 1871088, "en": "The aim of the current study was to investigate whether degradation of rapeseed meal (RSM) by a swine gut microbiota consortium was improved by modifying RSM by treatment with cellulase (CELL), two pectinases (PECT), or alkaline (ALK) compared to untreated RSM and to assess whether microbiota composition and activity changed. The predicted relative abundances of carbohydrate digestion and absorption, glycolysis, pentose phosphate pathway, and pyruvate metabolism were significantly increased upon CELL and ALK feeding, and CELL and ALK also exhibited increased total short-chain fatty acid (SCFA) production compared to CON. Megasphaera, Prevotella, and Desulfovibrio were significantly positively correlated with SCFA production. Findings were validated in ileal cannulated pigs, which showed that CELL and ALK increased fiber degradation of RSM. In conclusion, CELL and ALK rather than PECT1 or PECT2 increased fiber degradation in RSM, and this information could guide feed additive strategies to improve efficiency and productivity in the swine industry.", "gemini": "Mục đích của nghiên cứu hiện tại là điều tra xem quá trình phân hủy bột hạt cải (RSM) bởi một nhóm vi khuẩn đường ruột của lợn có được cải thiện bằng cách biến đổi RSM bằng cách xử lý bằng cellulase (CELL), hai pectinase (PECT) hoặc kiềm (ALK) so với RSM chưa xử lý hay không. và để đánh giá xem thành phần và hoạt động của hệ vi sinh vật có thay đổi hay không. Sự phong phú tương đối dự đoán của quá trình tiêu hóa và hấp thụ carbohydrate, đường phân giải, con đường pentose phosphate và chuyển hóa pyruvate tăng đáng kể khi cho ăn CELL và ALK, và CELL và ALK cũng cho thấy tổng sản xuất axit béo chuỗi ngắn (SCFA) tăng so với CON. Megasphaera, Prevotella và Desulfovibrio có mối tương quan dương đáng kể với sản xuất SCFA. Các phát hiện đã được xác nhận ở những con lợn có lỗ mở hồi tràng, cho thấy CELL và ALK làm tăng quá trình phân hủy chất xơ của RSM. Tóm lại, CELL và ALK chứ không phải PECT1 hoặc PECT2 làm tăng quá trình phân hủy chất xơ trong RSM và thông tin này có thể hướng dẫn các chiến lược bổ sung thức ăn để cải thiện hiệu quả và năng suất trong ngành chăn nuôi lợn."} +{"idx": 1871089, "en": "Zircon (ZrSiO4, I41/amd) can accommodate actinides, such as thorium, uranium, and plutonium. The zircon structure has been determined for several of the end-member compositions of other actinides, such as plutonium and neptunium. However, the thermodynamic properties of these actinide zircon structure types are largely unknown due to the difficulties in synthesizing these materials and handling transuranium actinides. Thus, we have completed a thermodynamic study of cerium orthosilicate, stetindite (CeSiO4), a surrogate of PuSiO4. For the first time, the standard enthalpy of formation of CeSiO4 was obtained by high temperature oxide melt solution calorimetry to be -1971.9 ± 3.6 kJ/mol. Stetindite is energetically metastable with respect to CeO2 and SiO2 by 27.5 ± 3.1 kJ/mol. The metastability explains the rarity of the natural occurrence of stetindite and the difficulty of its synthesis. Applying the obtained enthalpy of formation of CeSiO4 from this work, along with those previously reported for USiO4 and ThSiO4, we developed an empirical energetic relation for actinide orthosilicates. The predicted enthalpies of formation of AnSiO4 are then determined with a discussion of future strategies for efficiently immobilizing Pu or minor actinides in the zircon structure.", "gemini": "Zircon (ZrSiO4, I41/amd) có thể chứa các actinide, chẳng hạn như thorium, uranium và plutonium. Cấu trúc zircon đã được xác định cho một số thành phần cuối của các actinide khác, chẳng hạn như plutonium và neptunium. Tuy nhiên, các tính chất nhiệt động lực học của các loại cấu trúc zircon actinide này phần lớn vẫn chưa được biết do những khó khăn trong việc tổng hợp các vật liệu này và xử lý các actinide transuranium. Do đó, chúng tôi đã hoàn thành một nghiên cứu nhiệt động lực học về silicat orthosilicate, stetindite (CeSiO4), một chất thay thế của PuSiO4. Lần đầu tiên, enthalpy chuẩn tạo thành của CeSiO4 thu được bằng phương pháp nhiệt lượng kế dung dịch nóng chảy oxit nhiệt độ cao là -1971,9 ± 3,6 kJ/mol. Stetindite không ổn định về mặt năng lượng đối với CeO2 và SiO2 là 27,5 ± 3,1 kJ/mol. Sự không ổn định giải thích cho sự hiếm gặp của stetindite tự nhiên và khó khăn trong quá trình tổng hợp của nó. Áp dụng enthalpy tạo thành của CeSiO4 thu được từ công trình này, cùng với những enthalpy đã được báo cáo trước đó cho USiO4 và ThSiO4, chúng tôi đã phát triển một mối quan hệ năng lượng thực nghiệm cho các silicat orthosilicate actinide. Sau đó, các enthalpy tạo thành dự đoán của AnSiO4 được xác định với một cuộc thảo luận về các chiến lược trong tương lai để cố định hiệu quả Pu hoặc các actinide nhỏ trong cấu trúc zircon."} +{"idx": 1871090, "en": "A new reactive force field based on the ReaxFF formalism is effectively parametrized against an extended training set of quantum chemistry data (containing more than 120 different structures) to describe accurately silver and silver-thiolate systems. The results obtained with this novel representation demonstrate that the novel ReaxFF paradigm is a powerful methodology to reproduce more appropriately average geometric and energetic properties of metal clusters and slabs when compared to the earlier ReaxFF parametrizations dealing with silver and gold. ReaxFF cannot describe adequately specific geometrical features such as the observed shorter distances between the under-coordinated atoms at the cluster edges. Geometric and energetic properties of thiolates adsorbed on a silver Ag20 pyramid are correctly represented by the new ReaxFF and compared with results for gold. The simulation of self-assembled monolayers of thiolates on a silver (111) surface does not indicate the formation of staples in contrast to the results for gold-thiolate systems.", "gemini": "Một trường lực phản ứng mới dựa trên công thức ReaxFF được tham số hóa hiệu quả so với một tập dữ liệu huấn luyện mở rộng về dữ liệu hóa học lượng tử (chứa hơn 120 cấu trúc khác nhau) để mô tả chính xác các hệ bạc và bạc-thiolate. Các kết quả thu được với biểu diễn mới này chứng minh rằng mô hình ReaxFF mới là một phương pháp mạnh mẽ để tái tạo các tính chất hình học và năng lượng trung bình phù hợp hơn của các cụm và phiến kim loại khi so sánh với các tham số hóa ReaxFF trước đó liên quan đến bạc và vàng. ReaxFF không thể mô tả đầy đủ các đặc điểm hình học cụ thể như khoảng cách ngắn hơn quan sát được giữa các nguyên tử được phối hợp dưới tại các cạnh cụm. Các tính chất hình học và năng lượng của thiolate được hấp phụ trên một kim tự tháp Ag20 bạc được biểu diễn chính xác bởi ReaxFF mới và so sánh với các kết quả đối với vàng. Mô phỏng các lớp đơn tự lắp ráp của thiolate trên bề mặt bạc (111) không chỉ ra sự hình thành các ghim trái ngược với các kết quả đối với hệ thống vàng-thiolate."} +{"idx": 1871091, "en": "The CblC and CblD chaperones are involved in early steps in the cobalamin trafficking pathway. Cobalamin derivatives entering the cytoplasm are converted by CblC to a common cob(II)alamin intermediate via glutathione-dependent alkyltransferase or reductive elimination activities. Cob(II)alamin is subsequently converted to one of two biologically active alkylcobalamins by downstream chaperones. The function of CblD has been elusive although it is known to form a complex with CblC under certain conditions. Here, we report that CblD provides a sulfur ligand to cob(II)alamin bound to CblC, forming an interprotein coordination complex that rapidly oxidizes to thiolato-cob(III)alamin. Cysteine scanning mutagenesis and EPR spectroscopy identified Cys-261 on CblD as the sulfur donor. The unusual interprotein Co-S bond was characterized by X-ray absorption spectroscopy and visualized in the crystal structure of the human CblD thiolato-cob(III)alamin complex. Our study provides insights into how cobalamin coordination chemistry could be utilized for cofactor translocation in the trafficking pathway.", "gemini": "Các chaperone CblC và CblD tham gia vào các bước đầu tiên trong con đường vận chuyển cobalamin. Các dẫn xuất cobalamin đi vào tế bào chất được CblC chuyển đổi thành một chất trung gian cob(II)alamin phổ biến thông qua các hoạt động alkyltransferase phụ thuộc glutathione hoặc loại bỏ khử. Sau đó, Cob(II)alamin được chuyển đổi thành một trong hai alkylcobalamin hoạt động sinh học bởi các chaperone hạ nguồn. Chức năng của CblD vẫn còn khó nắm bắt mặc dù nó được biết là tạo thành một phức hợp với CblC trong một số điều kiện nhất định. Ở đây, chúng tôi báo cáo rằng CblD cung cấp một phối tử lưu huỳnh cho cob(II)alamin liên kết với CblC, tạo thành một phức hợp phối hợp liên protein nhanh chóng oxy hóa thành thiolato-cob(III)alamin. Đột biến quét cysteine và phổ EPR xác định Cys-261 trên CblD là chất cho lưu huỳnh. Liên kết Co-S liên protein bất thường được đặc trưng bằng phổ hấp thụ tia X và được hình dung trong cấu trúc tinh thể của phức hợp thiolato-cob(III)alamin CblD của người. Nghiên cứu của chúng tôi cung cấp thông tin chi tiết về cách hóa học phối hợp cobalamin có thể được sử dụng để chuyển vị cofactor trong con đường vận chuyển."} +{"idx": 1871092, "en": "Resistance to the last-line polymyxins is increasingly reported in multidrug-resistant Gram-negative pathogens, including Acinetobacter baumannii, which develops resistance via either lipid A modification (e.g., with phosphoethanolamine [pEtN]) or even lipopolysaccharide (LPS) loss in the outer membrane (OM). Considering these two different mechanisms, quantitative membrane lipidomics data were utilized to develop three OM models representing polymyxin-susceptible and -resistant A. baumannii strains. Through all-atom molecular simulations with enhanced sampling techniques, the effect of lipid A-pEtN modification and LPS loss on the action of colistin (i.e., polymyxin E) was examined for the first time, with a focus on the dynamics and energetics of colistin penetration into these OMs. Lipid A-pEtN modification improved the OM stability, impeding the penetration of colistin into the OM; this differed from the current literature that lipid A-pEtN modification confers resistance by diminishing the initial interaction with polymyxins. In contrast, the LPS deficiency significantly reduced the negative charges on the OM surface, diminishing the binding of colistin. Moreover, both lipid A-pEtN modification and LPS loss also constituted colistin resistance through disturbing the conformational transitions of the colistin molecule. Collectively, atomic-scale interactions between polymyxins and different bacterial OMs are very different and the findings may facilitate the discovery of new-generation polymyxins against Gram-negative 'superbugs'.", "gemini": "Kháng với polymyxin thế hệ cuối ngày càng được báo cáo ở các tác nhân gây bệnh Gram âm đa kháng thuốc, bao gồm Acinetobacter baumannii, phát triển khả năng kháng thông qua biến đổi lipid A (ví dụ, với phosphoethanolamine [pEtN]) hoặc thậm chí mất lipopolysaccharide (LPS) ở màng ngoài (OM). Xem xét hai cơ chế khác nhau này, dữ liệu lipidomics màng định lượng đã được sử dụng để phát triển ba mô hình OM đại diện cho các chủng A. baumannii nhạy cảm và kháng polymyxin. Thông qua mô phỏng phân tử toàn nguyên tử với các kỹ thuật lấy mẫu nâng cao, tác dụng của biến đổi lipid A-pEtN và mất LPS đối với tác dụng của colistin (tức là polymyxin E) đã được kiểm tra lần đầu tiên, tập trung vào động lực học và năng lượng của sự thâm nhập của colistin vào các OM này. Biến đổi lipid A-pEtN cải thiện độ ổn định của OM, cản trở sự thâm nhập của colistin vào OM; điều này khác với các tài liệu hiện tại cho rằng biến đổi lipid A-pEtN mang lại khả năng kháng bằng cách làm giảm tương tác ban đầu với polymyxin. Ngược lại, sự thiếu hụt LPS làm giảm đáng kể các điện tích âm trên bề mặt OM, làm giảm sự liên kết của colistin. Hơn nữa, cả biến đổi lipid A-pEtN và mất LPS cũng tạo nên khả năng kháng colistin thông qua việc làm nhiễu loạn các chuyển đổi cấu hình của phân tử colistin. Nhìn chung, các tương tác ở quy mô nguyên tử giữa polymyxin và các OM vi khuẩn khác nhau rất khác nhau và những phát hiện này có thể tạo điều kiện thuận lợi cho việc phát hiện ra các polymyxin thế hệ mới chống lại 'siêu vi khuẩn' Gram âm."} +{"idx": 1871093, "en": "An intriguing challenge of drug discovery is targeting pathogenic mutant proteins that differ from their wild-type counterparts by only a single amino acid. In particular, pathogenic cysteine mutations afford promising opportunities for mutant-specific drug discovery, due to the unique reactivity of cysteine's sulfhydryl-containing side chain. Here we describe the first directed discovery effort targeting a pathogenic cysteine mutant of a protein tyrosine phosphatase (PTP), namely Y279C Src-homology-2-containing PTP 2 (SHP2), which has been causatively linked to the developmental disorder Noonan syndrome with multiple lentigines (NSML). Through a screen of commercially available compounds that contain cysteine-reactive functional groups, we have discovered a small-molecule inhibitor of Y279C SHP2 (compound 99; IC50 ≈ 6 μM) that has no appreciable effect on the phosphatase activity of wild-type SHP2 or that of other homologous PTPs (IC50 ≫ 100 μM). Compound 99 exerts its specific inhibitory effect through irreversible engagement of Y279C SHP2's pathogenic cysteine residue in a manner that is time-dependent, is substrate-independent, and persists in the context of a complex proteome. To the best of our knowledge, 99 is the first specific ligand of a disease-causing PTP mutant to be identified. This study therefore provides both a starting point for the development of NSML-directed therapeutic agents and a precedent for the identification of mutant-specific inhibitors of other pathogenic PTP mutants.", "gemini": "Một thách thức hấp dẫn của việc khám phá thuốc là nhắm mục tiêu vào các protein đột biến gây bệnh khác với các protein kiểu hoang dã chỉ bằng một axit amin duy nhất. Đặc biệt, các đột biến cysteine gây bệnh mang lại cơ hội đầy hứa hẹn cho việc phát hiện thuốc đặc hiệu cho đột biến, do tính phản ứng độc đáo của chuỗi bên chứa sulfhydryl của cysteine. Tại đây, chúng tôi mô tả nỗ lực khám phá có định hướng đầu tiên nhắm vào một đột biến cysteine gây bệnh của một protein tyrosine phosphatase (PTP), cụ thể là Y279C Src-homology-2 có chứa PTP 2 (SHP2), có liên quan đến nguyên nhân gây ra rối loạn phát triển hội chứng Noonan với nhiều lentigo (NSML). Thông qua sàng lọc các hợp chất có sẵn trên thị trường có chứa các nhóm chức phản ứng với cysteine, chúng tôi đã phát hiện ra một chất ức chế phân tử nhỏ của Y279C SHP2 (hợp chất 99; IC50 ≈ 6 μM) không có tác dụng đáng kể đối với hoạt động phosphatase của SHP2 kiểu hoang dã hoặc các PTP đồng đẳng khác (IC50 ≫ 100 μM). Hợp chất 99 phát huy tác dụng ức chế đặc hiệu của mình thông qua sự tương tác không hồi phục với gốc cysteine gây bệnh của Y279C SHP2 theo cách phụ thuộc thời gian, không phụ thuộc vào chất nền và tồn tại trong bối cảnh của một proteome phức tạp. Theo hiểu biết của chúng tôi, 99 là phối tử đặc hiệu đầu tiên của một đột biến PTP gây bệnh được xác định. Do đó, nghiên cứu này cung cấp cả điểm khởi đầu cho sự phát triển của các tác nhân điều trị hướng đến NSML và tiền lệ cho việc xác định các chất ức chế đặc hiệu đột biến của các đột biến PTP gây bệnh khác."} +{"idx": 1871094, "en": "Three-dimensional printing (3DP) has attracted significant attention for its use in additive manufacturing techniques because it provides customizability and flexibility for fabricating structures with arbitrary shapes. Certain applications in the food and medicine industries require 3D printable materials that are both biocompatible and biodegradable. Consequently, this study reports 3D printable materials constructed from food-grade high internal phase emulsions (HIPEs). The studied HIPEs (phase ratio 85%) were stabilized by the efficient adsorption behavior of cod proteins (concentration range, 10-50 mg mL-1) at the oil-water interface. The stability of the oil-in-water HIPEs was improved by the formation of a concentration-dependent percentage of adsorbed proteins and cross-linking networks, and homogeneous and self-supporting structures were generated after 7 days of storage at 4 °C. The gel-like shear thinning rheological behavior induced by the cross-linking networks in the studied HIPEs can be tuned to obtain the desired printability and extrudability during 3DP. In the present study, the HIPEs stabilized with 50 mg mL-1 of cod proteins exhibited the highest printing resolution, gel strength, hardness, adhesiveness, and chewiness during 3DP. These food-grade HIPE inks have the potential to diversify the applications of 3DP in foods, cosmetics, drug delivery systems, and packaging materials.", "gemini": "In 3D (3DP) đã thu hút sự chú ý đáng kể vì được sử dụng trong các kỹ thuật sản xuất bồi đắp vì nó cung cấp khả năng tùy chỉnh và linh hoạt để chế tạo các cấu trúc có hình dạng tùy ý. Một số ứng dụng trong ngành thực phẩm và y học đòi hỏi vật liệu in 3D vừa tương thích sinh học vừa có thể phân hủy sinh học. Do đó, nghiên cứu này báo cáo về các vật liệu in 3D được chế tạo từ nhũ tương pha trong cao cấp (HIPE) cấp thực phẩm. Các HIPE được nghiên cứu (tỷ lệ pha 85%) được ổn định bởi hành vi hấp phụ hiệu quả của protein cá tuyết (phạm vi nồng độ 10-50 mg mL-1) ở giao diện dầu-nước. Độ ổn định của HIPE dầu trong nước được cải thiện nhờ sự hình thành tỷ lệ phần trăm protein hấp phụ phụ thuộc vào nồng độ và mạng lưới liên kết ngang, và các cấu trúc đồng nhất và tự nâng đỡ được tạo ra sau 7 ngày bảo quản ở 4 °C. Hành vi lưu biến loãng cắt giống gel do các mạng liên kết ngang trong các HIPE được nghiên cứu gây ra có thể được điều chỉnh để đạt được khả năng in và đùn mong muốn trong quá trình 3DP. Trong nghiên cứu hiện tại, các HIPE được ổn định với 50 mg mL-1 protein cá tuyết thể hiện độ phân giải in, độ bền gel, độ cứng, độ dính và độ dai cao nhất trong quá trình 3DP. Các loại mực HIPE cấp thực phẩm này có tiềm năng đa dạng hóa các ứng dụng của 3DP trong thực phẩm, mỹ phẩm, hệ thống phân phối thuốc và vật liệu đóng gói."} +{"idx": 1871095, "en": "High-energy resolution fluorescence-detected Cu K-edge X-ray absorption spectroscopy (XAS) and single-crystal polarized XAS data are presented toward refining the assignments of bands assigned as excitations from Cu 1s to ligand-localized molecular orbitals. These have been previously dubbed \"XAS-metal-ligand charge transfer\" (XAS-MLCT) bands. Data are presented for a series of [Cu(xbpy)2] complexes (xbpy = 2,2'-bipyridine (1), 4,4'-bisamino-2,2'-bipyridine (2), and 4,4'-dimethoxy-2,2'-bipyridine (3); n = 1 and 2). Dipolar dependencies of these \"XAS-MLCT\" bands in both Cu1+ and Cu2+ species lead to reassignment of these features as owing their intensities primarily to Cu 1s → Cu 4p excitations. The transition densities are Cu-localized, highlighting that XAS-MLCT features in Cu XAS spectra are not \"charge transfer\" transitions but rather quasi-atomic transitions. Although scrutiny of the acceptor orbitals supports assignment as Cu 1s → ligand π* transitions, it ultimately appears that while the ligand orbital energetics govern the positions of these bands the intensity is conferred through a small degree of metal 4p mixing into otherwise ligand-dominated acceptor molecular orbitals.", "gemini": "Phổ hấp thụ tia X vùng K-Cu phát hiện huỳnh quang độ phân giải năng lượng cao (XAS) và dữ liệu XAS phân cực tinh thể đơn được trình bày để tinh chỉnh các phép gán dải được chỉ định là sự kích thích từ Cu 1s đến các quỹ đạo phân tử cục bộ của phối tử. Trước đây, chúng được gọi là dải \"chuyển điện tích kim loại-phối tử XAS\" (XAS-MLCT). Dữ liệu được trình bày cho một loạt các hợp chất [Cu(xbpy)2] (xbpy = 2,2'-bipyridine (1), 4,4'-bisamino-2,2'-bipyridine (2) và 4,4'-dimethoxy-2,2'-bipyridine (3); n = 1 và 2). Sự phụ thuộc lưỡng cực của các dải \"XAS-MLCT\" này ở cả các loài Cu1+ và Cu2+ dẫn đến việc chỉ định lại các đặc điểm này là do cường độ của chúng chủ yếu là do các kích thích Cu 1s → Cu 4p. Mật độ chuyển tiếp được định vị tại Cu, làm nổi bật rằng các đặc điểm XAS-MLCT trong phổ XAS Cu không phải là các chuyển tiếp \"chuyển điện tích\" mà đúng hơn là các chuyển tiếp gần như nguyên tử. Mặc dù việc xem xét các quỹ đạo chấp nhận hỗ trợ việc chỉ định là các chuyển tiếp Cu 1s → phối tử π*, nhưng cuối cùng có vẻ như trong khi năng lượng quỹ đạo phối tử chi phối vị trí của các dải này thì cường độ được truyền đạt thông qua một mức độ nhỏ của kim loại 4p trộn vào các quỹ đạo phân tử chấp nhận bị phối tử chi phối."} +{"idx": 1871096, "en": "Most of our understanding of chemistry derives from atomic-level structures obtained with single-crystal X-ray diffraction. Metal centers in X-ray structures of small organometallic or coordination complexes are often extremely well-defined, with errors in the positions on the order of 10-4-10-5 Å. Determining the metal coordination geometry to high accuracy is essential for understanding metal center reactivity, as even small structural changes can dramatically alter the metal activity. In contrast, the resolution of X-ray structures in proteins is limited typically to the order of 10-1 Å. This resolution is often not sufficient to develop precise structure-activity relations for the metal sites in proteins, because the uncertainty in positions can cover all of the known ranges of bond lengths and bond angles for a given type of metal complex. Here we introduce a new approach that enables the determination of a high-definition structure of the active site of a metalloprotein from a powder sample, by combining magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, tailored radio frequency (RF) irradiation schemes, and computational approaches. This allows us to overcome the \"blind sphere\" in paramagnetic proteins, and to observe and assign 1H, 13C, and 15N resonances for the ligands directly coordinating the metal center. We illustrate the method by determining the bond lengths in the structure of the CoII coordination sphere at the core of human superoxide dismutase 1 (SOD) with 0.7 pm precision. The coordination geometry of the resulting structure explains the nonreactive nature of the CoII/ZnII centers in these proteins, which allows them to play a purely structural role.", "gemini": "Hầu hết hiểu biết của chúng ta về hóa học đều bắt nguồn từ các cấu trúc cấp độ nguyên tử thu được bằng nhiễu xạ tia X tinh thể đơn. Các trung tâm kim loại trong các cấu trúc tia X của các hợp chất organometallic hoặc phức hợp phối hợp nhỏ thường được xác định cực kỳ tốt, với sai số về vị trí theo thứ tự 10-4-10-5 Å. Việc xác định hình dạng phối hợp kim loại với độ chính xác cao là rất cần thiết để hiểu phản ứng của trung tâm kim loại, vì ngay cả những thay đổi nhỏ về cấu trúc cũng có thể làm thay đổi đáng kể hoạt động của kim loại. Ngược lại, độ phân giải của các cấu trúc tia X trong protein thường chỉ giới hạn ở bậc 10-1 Å. Độ phân giải này thường không đủ để phát triển các mối quan hệ chính xác về cấu trúc-hoạt động cho các vị trí kim loại trong protein, vì độ không chắc chắn về vị trí có thể bao phủ tất cả các phạm vi đã biết về độ dài liên kết và góc liên kết cho một loại phức hợp kim loại nhất định. Tại đây, chúng tôi giới thiệu một phương pháp tiếp cận mới cho phép xác định cấu trúc độ nét cao của vị trí hoạt động của một metalloprotein từ mẫu bột, bằng cách kết hợp phép quay góc ma thuật (MAS) phổ cộng hưởng từ hạt nhân (NMR), các lược đồ chiếu tần số vô tuyến (RF) được thiết kế riêng và các phương pháp tính toán. Điều này cho phép chúng ta vượt qua \"vùng mù\" trong các protein thuận từ, và quan sát và chỉ định các cộng hưởng 1H, 13C và 15N cho các phối tử phối hợp trực tiếp với trung tâm kim loại. Chúng tôi minh họa phương pháp bằng cách xác định độ dài liên kết trong cấu trúc của vùng phối hợp CoII tại lõi của superoxide dismutase 1 của người (SOD) với độ chính xác 0,7 pm. Hình dạng phối hợp của cấu trúc kết quả giải thích bản chất không phản ứng của các trung tâm CoII/ZnII trong các protein này, cho phép chúng đóng vai trò hoàn toàn về mặt cấu trúc."} +{"idx": 1871097, "en": "T cells have a central role in immune system balance. When activated, they may lead to autoimmune diseases. When too anergic, they contribute to infection spread and cancer proliferation. Immune checkpoint proteins regulate T cell function, including cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed cell death-1 (PD-1) and its ligand (PD-L1). These nodes of self-tolerance may be exploited pharmacologically to downregulate (CTLA-4 agonists) and activate [CTLA-4 and PD-1/PD-L1 antagonists, also called immune checkpoint inhibitors (ICIs)] the immune system.CTLA-4 agonists are used to treat rheumatologic immune disorders and graft rejection. CTLA-4, PD-1, and PD-L1 antagonists are approved for multiple cancer types and are being investigated for chronic viral infections. Notably, ICIs may be associated with immune-related adverse events (irAEs), which can be highly morbid or fatal. CTLA-4 agonism has been a promising method to reverse such life-threatening irAEs. Herein, we review the clinical pharmacology of these immune checkpoint agents with a focus on their interplay in human diseases.", "gemini": "Tế bào T có vai trò trung tâm trong sự cân bằng của hệ thống miễn dịch. Khi được kích hoạt, chúng có thể dẫn đến các bệnh tự miễn. Khi quá ức chế, chúng góp phần vào sự lây lan của nhiễm trùng và sự phát triển của ung thư. Các protein điểm kiểm miễn dịch điều chỉnh chức năng tế bào T, bao gồm kháng nguyên tế bào lympho T độc tế bào-4 (CTLA-4) và tế bào chết theo chương trình-1 (PD-1) và phối tử của nó (PD-L1). Các nút tự dung nạp này có thể được khai thác về mặt dược lý để điều chỉnh giảm (chất chủ vận CTLA-4) và kích hoạt [chất đối kháng CTLA-4 và PD-1/PD-L1, còn được gọi là thuốc ức chế điểm kiểm miễn dịch (ICI)] hệ thống miễn dịch. Chất chủ vận CTLA-4 được sử dụng để điều trị các rối loạn miễn dịch thấp khớp và thải ghép. Các chất đối kháng CTLA-4, PD-1 và PD-L1 được chấp thuận cho nhiều loại ung thư và đang được nghiên cứu cho các bệnh nhiễm trùng do vi-rút mạn tính. Đáng chú ý, ICI có thể liên quan đến các tác dụng phụ liên quan đến miễn dịch (irAE), có thể gây bệnh nặng hoặc tử vong. Chủ vận CTLA-4 là một phương pháp đầy hứa hẹn để đảo ngược các irAE đe dọa tính mạng như vậy. Trong bài này, chúng tôi xem xét dược lý lâm sàng của các tác nhân điểm kiểm miễn dịch này với trọng tâm là sự tương tác của chúng trong các bệnh của con người."} +{"idx": 1871098, "en": "For efficient use of limited capacity, the visual system summarizes redundant information and prioritizes relevant information, strategies known respectively as ensemble perception and selective attention. Although previous studies showed a close relationship between these strategies, the specific mechanisms underlying the relationship have not been determined. We investigated how attention modulated mean-size computation. Fourteen people participated in this study. We hypothesized that attention biases mean-size computation by increasing the contribution (weighted averaging) and the apparent size (perceptual enlargement) of an attended item. Consistent with this hypothesis, our results showed that estimated mean sizes were biased toward the attended size and overestimated regardless of the attended size, supporting weighted averaging and perceptual enlargement, respectively. Taken together, the observed effects of selective attention on mean-size computation signify a close relationship between the two optimization mechanisms to achieve efficient management of the visual system's limited capacity.", "gemini": "Để sử dụng hiệu quả năng lực hạn chế, hệ thống thị giác sẽ tóm tắt thông tin dư thừa và ưu tiên thông tin có liên quan, các chiến lược được gọi lần lượt là nhận thức tổng thể và sự chú ý có chọn lọc. Mặc dù các nghiên cứu trước đây cho thấy mối quan hệ chặt chẽ giữa các chiến lược này, nhưng các cơ chế cụ thể làm nền tảng cho mối quan hệ này vẫn chưa được xác định. Chúng tôi đã nghiên cứu cách chú ý điều chỉnh phép tính kích thước trung bình. Mười bốn người đã tham gia nghiên cứu này. Chúng tôi đưa ra giả thuyết rằng sự chú ý làm sai lệch phép tính kích thước trung bình bằng cách tăng sự đóng góp (trung bình có trọng số) và kích thước biểu kiến (phóng to về mặt nhận thức) của một mục được chú ý. Phù hợp với giả thuyết này, kết quả của chúng tôi cho thấy kích thước trung bình ước tính bị lệch về phía kích thước được chú ý và được ước tính quá cao bất kể kích thước được chú ý, tương ứng hỗ trợ cho trung bình có trọng số và phóng to về mặt nhận thức. Xét về tổng thể, các tác động quan sát được của sự chú ý có chọn lọc đối với phép tính kích thước trung bình biểu thị mối quan hệ chặt chẽ giữa hai cơ chế tối ưu hóa để đạt được khả năng quản lý hiệu quả năng lực hạn chế của hệ thống thị giác."} +{"idx": 1871099, "en": "Botanists have long identified bilaterally symmetrical (zygomorphic) flowers with more specialized pollination interactions than radially symmetrical (actinomorphic) flowers. Zygomorphic flowers facilitate more precise contact with pollinators, guide pollinator behaviour and exclude less effective pollinators. However, whether zygomorphic flowers are actually visited by a smaller subset of available pollinator species has not been broadly evaluated. We compiled 53 609 floral visitation records in 159 communities and classified the plants' floral symmetry. Globally and within individual communities, plants with zygomorphic flowers are indeed visited by fewer species. At the same time, zygomorphic flowers share a somewhat larger proportion of their visitor species with other co-occurring plants and have particularly high sharing with co-occurring plants that also have zygomorphic flowers. Visitation sub-networks for zygomorphic species also show differences that may arise from reduced visitor diversity, including greater connectance, greater web asymmetry and lower coextinction robustness of both plants and visitor species-but these changes do not necessarily translate to whole plant-visitor communities. These results provide context for widely documented associations between zygomorphy and diversification and imply that species with zygomorphic flowers may face a greater risk of extinction due to pollinator loss.", "gemini": "Các nhà thực vật học từ lâu đã xác định được hoa đối xứng hai bên (đối xứng zygomorphic) có sự tương tác thụ phấn chuyên biệt hơn so với hoa đối xứng xuyên tâm (đối xứng actinomorphic). Hoa đối xứng zygomorphic tạo điều kiện tiếp xúc chính xác hơn với các loài thụ phấn, hướng dẫn hành vi thụ phấn và loại trừ các loài thụ phấn kém hiệu quả hơn. Tuy nhiên, liệu hoa đối xứng zygomorphic có thực sự được ít loài thụ phấn trong số các loài thụ phấn khả dụng ghé thăm hay không vẫn chưa được đánh giá rộng rãi. Chúng tôi đã biên soạn 53.609 hồ sơ ghé thăm hoa tại 159 cộng đồng và phân loại tính đối xứng của hoa cây. Trên toàn cầu và trong từng cộng đồng riêng lẻ, các loài thực vật có hoa đối xứng zygomorphic thực sự được ít loài ghé thăm hơn. Đồng thời, hoa đối xứng zygomorphic chia sẻ tỷ lệ các loài khách cao hơn một chút với các loài thực vật cùng xuất hiện khác và chia sẻ đặc biệt cao với các loài thực vật cùng xuất hiện cũng có hoa đối xứng zygomorphic. Các mạng lưới con ghé thăm đối với các loài đối xứng zygomorphic cũng cho thấy sự khác biệt có thể phát sinh do sự đa dạng khách ghé thăm giảm, bao gồm kết nối lớn hơn, tính không đối xứng của mạng lớn hơn và khả năng phục hồi đồng tuyệt chủng thấp hơn của cả thực vật và các loài khách ghé thăm - nhưng những thay đổi này không nhất thiết chuyển thành toàn bộ cộng đồng thực vật-khách ghé thăm. Những kết quả này cung cấp bối cảnh cho các mối liên hệ được ghi chép rộng rãi giữa tính đối xứng zygomorphic và sự đa dạng hóa và ngụ ý rằng các loài có hoa đối xứng zygomorphic có thể phải đối mặt với nguy cơ tuyệt chủng cao hơn do mất loài thụ phấn."} +{"idx": 1871100, "en": "Novel object trials are commonly used to assess aversion to novelty (neophobia), and previous work has shown neophobia can be influenced by the social environment, but whether the altered behaviour persists afterwards (social learning) is largely unknown in wild animals. We assessed house sparrow (Passer domesticus) novel object responses before, during and after being paired with a conspecific of either similar or different behavioural phenotype. During paired trials, animals housed with a similar or more neophobic partner demonstrated an increased aversion to novel objects. This change did not persist a week after unpairing, but neophobia decreased after unpairing in birds previously housed with a less neophobic partner. We also compared novel object responses to non-object control trials to validate our experimental procedure. Our results provide evidence of social learning in a highly successful invasive species, and an interesting asymmetry in the effects of social environment on neophobia behaviour depending on the animal's initial behavioural phenotype.", "gemini": "Các thử nghiệm đối tượng mới thường được sử dụng để đánh giá sự ác cảm với sự mới lạ (sợ mới), và các nghiên cứu trước đây đã chỉ ra rằng sợ mới có thể bị ảnh hưởng bởi môi trường xã hội, nhưng liệu hành vi thay đổi có tiếp tục sau đó (học tập xã hội) hay không thì phần lớn vẫn chưa được biết ở động vật hoang dã. Chúng tôi đã đánh giá phản ứng đối tượng mới của chim sẻ nhà (Passer domesticus) trước, trong và sau khi được ghép đôi với một loài có kiểu hình hành vi tương tự hoặc khác nhau. Trong các thử nghiệm ghép đôi, những con vật được nuôi chung với một đối tác có kiểu hình sợ mới tương tự hoặc nhiều hơn đã thể hiện sự ác cảm gia tăng đối với các vật thể mới. Sự thay đổi này không kéo dài một tuần sau khi tách đôi, nhưng chứng sợ mới đã giảm sau khi tách đôi ở những con chim trước đây được nuôi chung với một đối tác ít sợ mới hơn. Chúng tôi cũng đã so sánh phản ứng đối tượng mới với các thử nghiệm đối chứng không phải đối tượng để xác nhận quy trình thử nghiệm của chúng tôi. Kết quả của chúng tôi cung cấp bằng chứng về sự học tập xã hội ở một loài xâm lấn rất thành công và một sự không đối xứng thú vị trong các tác động của môi trường xã hội đối với hành vi sợ mới tùy thuộc vào kiểu hình hành vi ban đầu của động vật."} +{"idx": 1871101, "en": "Invasive alien species are driving global biodiversity loss, compromising ecosystem function and service provision, and human, animal and plant health. Habitat characteristics and geographical origin may predict invasion success, and in aquatic environments could be mediated principally by salinity tolerance. Crustacean invaders are causing global problems and we urgently require better predictive power of their invasiveness. Here, we compiled global aquatic gammarid (Crustacea: Amphipoda: Gammaroidea) diversity and examined their salinity tolerances and regions of origin to test whether these factors predict invasion success. Across 918 aquatic species within this superfamily, relatively few gammarids (n = 27, 3%) were reported as aliens, despite extensive invasion opportunities and high numbers of published studies on amphipod invasions. However, reported alien species were disproportionately salt-tolerant (i.e. 32% of brackish-water species), with significantly lower proportions of aliens originating from freshwater and marine environments (both 1%). Alien gammarids also significantly disproportionally originated from the Ponto-Caspian (20% of these taxa) when compared with all 'other' grouped regions (1%), and principally invaded Eurasian waters, with translocations of salt-tolerant taxa to freshwaters being pervasive. This suggests habitat characteristics, alongside regional contexts, help predict invasibility. In particular, broad environmental tolerances to harsh environments and associated evolutionary history probably promote success of aliens globally.", "gemini": "Các loài ngoại lai xâm lấn đang thúc đẩy mất đa dạng sinh học toàn cầu, làm tổn hại đến chức năng của hệ sinh thái và việc cung cấp dịch vụ, sức khỏe của con người, động vật và thực vật. Đặc điểm môi trường sống và nguồn gốc địa lý có thể dự đoán được sự thành công của cuộc xâm lược và trong môi trường nước có thể được trung gian chủ yếu bởi khả năng chịu mặn. Những kẻ xâm lược giáp xác đang gây ra những vấn đề toàn cầu và chúng ta cần gấp rút nâng cao khả năng dự đoán sức mạnh xâm lược của chúng. Ở đây, chúng tôi đã biên soạn sự đa dạng của loài giáp xác thủy sinh toàn cầu (Crustacea: Amphipoda: Gammaroidea) và kiểm tra khả năng chịu mặn và vùng xuất xứ của chúng để kiểm tra xem các yếu tố này có dự đoán được sự thành công của cuộc xâm lược hay không. Trong số 918 loài thủy sinh trong siêu họ này, tương đối ít loài giáp xác (n = 27, 3%) được báo cáo là loài ngoại lai, mặc dù có nhiều cơ hội xâm lược và số lượng lớn các nghiên cứu đã công bố về cuộc xâm lược của loài chân đều. Tuy nhiên, các loài ngoại lai được báo cáo lại có khả năng chịu mặn không cân xứng (tức là 32% loài nước lợ), với tỷ lệ loài ngoại lai có nguồn gốc từ môi trường nước ngọt và biển thấp hơn đáng kể (cả hai đều là 1%). Các loài giáp xác ngoại lai cũng có nguồn gốc không cân xứng đáng kể từ Ponto-Caspian (20% các loài này) khi so sánh với tất cả các vùng nhóm 'khác' (1%) và chủ yếu xâm chiếm vùng nước Á-Âu, với việc chuyển các loài chịu mặn sang vùng nước ngọt là phổ biến. Điều này cho thấy các đặc điểm môi trường sống, cùng với bối cảnh khu vực, giúp dự đoán khả năng xâm lược. Đặc biệt, khả năng chịu đựng môi trường rộng đối với môi trường khắc nghiệt và lịch sử tiến hóa liên quan có thể thúc đẩy sự thành công của các loài ngoại lai trên toàn cầu."} +{"idx": 1871102, "en": "Many pathogenic bacteria can protect themselves from the effects of antibiotics and the host immune response system by forming biofilms. Biofilms are polymer-entrapped bacterial cells, which adhere to each other and are often attached to a surface. Eradication of bacterial biofilms typically requires much higher concentrations of antibiotics than are normally needed to kill cultured planktonic cells, raising serious clinical concerns. In an attempt to prevent the formation of biofilms or to break up existing biofilms of pathogenic bacteria, herein we have used the standard crystal violet assay as well as the Calgary biofilm device to test several lactoferrin- and lactoferricin-derived antimicrobial peptides for their antibiofilm activity against Pseudomonas aeruginosa PAO1. Our results revealed that the short bovine lactoferricin-derived RRWQWR-NH2 (20-25) hexapeptide has no activity against P. aeruginosa PAO1. Moreover, the longer human lactoferricin-derived peptide GRRRRSVQWCA (1-11) and the bovine lactoferrampin (268-284) peptide were also almost devoid of activity. However, several different \"mix-and-match\" dimeric versions of the two lactoferricin-derived peptides proved quite effective in preventing the formation of biofilms at low concentrations, and in some cases, could even eradicate an existing biofilm. Moreover, the full-length bovine lactoferricinB (17-41) peptide also displayed considerable antimicrobial activity. Some of the longer lactoferricin-derived dimeric peptides acted through a bactericidal mechanism, whereas others seemed to interfere in cell-signalling processes. Taken together, our results indicate that synthetic dimeric peptides comprising short naturally occurring human and bovine lactoferricin constructs could be further developed as antibiofilm agents.", "gemini": "Nhiều vi khuẩn gây bệnh có thể tự bảo vệ mình khỏi tác động của thuốc kháng sinh và hệ thống phản ứng miễn dịch của vật chủ bằng cách hình thành màng sinh học. Màng sinh học là các tế bào vi khuẩn bị mắc kẹt trong polyme, bám vào nhau và thường bám vào một bề mặt. Việc diệt trừ màng sinh học của vi khuẩn thường đòi hỏi nồng độ thuốc kháng sinh cao hơn nhiều so với mức bình thường cần thiết để tiêu diệt các tế bào sinh vật phù du được nuôi cấy, gây ra những lo ngại nghiêm trọng về mặt lâm sàng. Trong nỗ lực ngăn ngừa sự hình thành màng sinh học hoặc phá vỡ các màng sinh học hiện có của vi khuẩn gây bệnh, tại đây chúng tôi đã sử dụng xét nghiệm tinh thể tím tiêu chuẩn cũng như thiết bị màng sinh học Calgary để thử nghiệm một số peptide kháng khuẩn có nguồn gốc từ lactoferrin và lactoferricin để chống lại màng sinh học của chúng. hoạt động chống lại Pseudomonas aeruginosa PAO1. Kết quả của chúng tôi cho thấy rằng hexapeptide RRWQWR-NH2 (20-25) có nguồn gốc từ lactoferricin ở bò ngắn không có hoạt tính chống lại P. aeruginosa PAO1. Hơn nữa, peptide GRRRRSVQWCA (1-11) có nguồn gốc từ lactoferricin ở người dài hơn và peptide lactoferrampin (268-284) ở bò cũng hầu như không có hoạt tính. Tuy nhiên, một số phiên bản dimeric \"trộn và kết hợp\" khác nhau của hai peptide có nguồn gốc từ lactoferricin đã chứng minh khá hiệu quả trong việc ngăn ngừa sự hình thành màng sinh học ở nồng độ thấp và trong một số trường hợp, thậm chí có thể diệt trừ màng sinh học hiện có. Hơn nữa, peptide lactoferricinB (17-41) ở bò có chiều dài đầy đủ cũng cho thấy hoạt tính kháng khuẩn đáng kể. Một số peptide dimeric có nguồn gốc từ lactoferricin dài hơn hoạt động thông qua cơ chế diệt khuẩn, trong khi một số khác có vẻ như can thiệp vào các quá trình truyền tín hiệu tế bào. Kết hợp lại, kết quả của chúng tôi chỉ ra rằng các peptide dimeric tổng hợp bao gồm các cấu trúc lactoferricin ở người và bò tự nhiên ngắn có thể được phát triển thành các tác nhân chống màng sinh học."} +{"idx": 1871103, "en": "The purpose of this study was to assess the effect of physical activity (PA) changes, measured by accelerometry, on telomere length (TL) in pediatric patients with abdominal obesity after a lifestyle intervention. One hundred and twenty-one children (7-16 years old) with abdominal obesity were randomized to the intervention (a moderately hypocaloric Mediterranean diet) or the usual care group (standard pediatric recommendations) for 22 months (a 2 month intensive phase and a subsequent 20 month follow-up). Both groups were encouraged to accumulate an extra 200 min/week of PA. TL was measured by MMqPCR. Data were analyzed in 102 subjects after 2 months and 64 subjects at the first 10 months of follow-up. Light PA level decreased in both groups after 12 months of intervention. At month 2, moderate to vigorous PA (MVPA) increased in the intervention group (+5.4 min/day, p = 0.035) and so did sedentary time in the usual care group (+49.7 min/day, p = 0.010). TL changes were positively associated (p < 0.050) with metabolic equivalents (METs), MVPA level, and number of steps, and were inversely associated with sedentary and light PA levels in the intervention group after the intensive phase. In conclusion, favourable changes in PA levels in the intensive phase of a lifestyle intervention could contribute to TL maintenance in a pediatric population with abdominal obesity. Novelty Changes in physical activity levels had a direct effect on telomere length, a biomarker of cellular aging and oxidative stress. PA advice based on The American College of Sports Medicine included in this intervention is easy to implement in primary care.", "gemini": "Mục đích của nghiên cứu này là đánh giá tác động của những thay đổi về hoạt động thể chất (PA), được đo bằng phép đo gia tốc, đến độ dài telomere (TL) ở bệnh nhi béo phì bụng sau khi can thiệp lối sống. Một trăm hai mươi mốt trẻ em (7-16 tuổi) bị béo phì bụng được phân ngẫu nhiên vào nhóm can thiệp (chế độ ăn Địa Trung Hải ít calo vừa phải) hoặc nhóm chăm sóc thông thường (khuyến cáo nhi khoa tiêu chuẩn) trong 22 tháng (giai đoạn tăng cường 2 tháng và giai đoạn tiếp theo 20 tháng theo dõi). Cả hai nhóm đều được khuyến khích tăng thêm 200 phút/tuần PA. TL được đo bằng MMqPCR. Dữ liệu được phân tích ở 102 đối tượng sau 2 tháng và 64 đối tượng trong 10 tháng theo dõi đầu tiên. Mức độ PA nhẹ giảm ở cả hai nhóm sau 12 tháng can thiệp. Vào tháng thứ 2, PA vừa phải đến mạnh (MVPA) tăng ở nhóm can thiệp (+5,4 phút/ngày, p = 0,035) và thời gian ngồi cũng tăng ở nhóm chăm sóc thông thường (+49,7 phút/ngày, p = 0,010). Những thay đổi về TL có liên quan thuận lợi (p < 0,050) với các tương đương trao đổi chất (MET), mức MVPA và số bước, và có liên quan nghịch đảo với mức độ PA tĩnh tại và nhẹ ở nhóm can thiệp sau giai đoạn tăng cường. Tóm lại, những thay đổi thuận lợi về mức độ PA trong giai đoạn tăng cường của can thiệp lối sống có thể góp phần duy trì TL trong quần thể nhi khoa bị béo phì bụng. Sự mới lạ Những thay đổi về mức độ hoạt động thể chất có tác động trực tiếp đến độ dài telomere, một dấu ấn sinh học của quá trình lão hóa tế bào và stress oxy hóa. Lời khuyên về PA dựa trên Học viện Y học Thể thao Hoa Kỳ được đưa vào can thiệp này rất dễ thực hiện trong chăm sóc ban đầu."} +{"idx": 1871104, "en": "A key requirement in studies of endemic vector-borne or zoonotic disease is an estimate of the spatial variation in vector or reservoir host abundance. For many vector species, multiple indices of abundance are available, but current approaches to choosing between or combining these indices do not fully exploit the potential inferential benefits that might accrue from modelling their joint spatial distribution. Here, we develop a class of multivariate generalized linear geostatistical models for multiple indices of abundance. We illustrate this novel methodology with a case study on Norway rats in a low-income urban Brazilian community, where rat abundance is a likely risk factor for human leptospirosis. We combine three indices of rat abundance to draw predictive inferences on a spatially continuous latent process, rattiness, that acts as a proxy for abundance. We show how to explore the association between rattiness and spatially varying environmental factors, evaluate the relative importance of each of the three contributing indices and assess the presence of residual, unexplained spatial variation, and identify rattiness hotspots. The proposed methodology is applicable more generally as a tool for understanding the role of vector or reservoir host abundance in predicting spatial variation in the risk of human disease.", "gemini": "Một yêu cầu quan trọng trong các nghiên cứu về bệnh do véc tơ lưu hành hoặc bệnh do động vật mang mầm bệnh là ước tính sự biến đổi không gian về số lượng vật chủ véc tơ hoặc vật chủ chứa mầm bệnh. Đối với nhiều loài véc tơ, có nhiều chỉ số về số lượng, nhưng các phương pháp tiếp cận hiện tại để lựa chọn giữa hoặc kết hợp các chỉ số này không khai thác đầy đủ các lợi ích suy luận tiềm năng có thể phát sinh từ việc lập mô hình phân phối không gian chung của chúng. Tại đây, chúng tôi phát triển một lớp mô hình địa thống kê tuyến tính tổng quát đa biến cho nhiều chỉ số về số lượng. Chúng tôi minh họa phương pháp luận mới này bằng một nghiên cứu trường hợp trên chuột Na Uy trong một cộng đồng đô thị thu nhập thấp của Brazil, nơi số lượng chuột có khả năng là yếu tố nguy cơ đối với bệnh leptospirosis ở người. Chúng tôi kết hợp ba chỉ số về số lượng chuột để đưa ra suy luận dự đoán về một quá trình tiềm ẩn liên tục về mặt không gian, sự ưa thích chuột, đóng vai trò là đại diện cho số lượng. Chúng tôi chỉ ra cách khám phá mối liên quan giữa sự ưa thích chuột và các yếu tố môi trường thay đổi theo không gian, đánh giá tầm quan trọng tương đối của từng chỉ số đóng góp trong ba chỉ số và đánh giá sự hiện diện của sự biến đổi không gian còn sót lại, chưa được giải thích và xác định các điểm nóng về sự ưa thích chuột. Phương pháp luận được đề xuất có thể áp dụng rộng rãi hơn như một công cụ để hiểu vai trò của số lượng vật chủ véc tơ hoặc vật chủ chứa mầm bệnh trong việc dự đoán sự biến đổi không gian về nguy cơ mắc bệnh ở người."} +{"idx": 1871105, "en": "Calcium (Ca2+) plays a central role in mediating both contractile function and hypertrophic signaling in ventricular cardiomyocytes. L-type Ca2+ channels trigger release of Ca2+ from ryanodine receptors for cellular contraction, whereas signaling downstream of G-protein-coupled receptors stimulates Ca2+ release via inositol 1,4,5-trisphosphate receptors (IP3Rs), engaging hypertrophic signaling pathways. Modulation of the amplitude, duration, and duty cycle of the cytosolic Ca2+ contraction signal and spatial localization have all been proposed to encode this hypertrophic signal. Given current knowledge of IP3Rs, we develop a model describing the effect of functional interaction (cross talk) between ryanodine receptor and IP3R channels on the Ca2+ transient and examine the sensitivity of the Ca2+ transient shape to properties of IP3R activation. A key result of our study is that IP3R activation increases Ca2+ transient duration for a broad range of IP3R properties, but the effect of IP3R activation on Ca2+ transient amplitude is dependent on IP3 concentration. Furthermore we demonstrate that IP3-mediated Ca2+ release in the cytosol increases the duty cycle of the Ca2+ transient, the fraction of the cycle for which [Ca2+] is elevated, across a broad range of parameter values and IP3 concentrations. When coupled to a model of downstream transcription factor (NFAT) activation, we demonstrate that there is a high correspondence between the Ca2+ transient duty cycle and the proportion of activated NFAT in the nucleus. These findings suggest increased cytosolic Ca2+ duty cycle as a plausible mechanism for IP3-dependent hypertrophic signaling via Ca2+-sensitive transcription factors such as NFAT in ventricular cardiomyocytes.", "gemini": "Canxi (Ca2+) đóng vai trò trung tâm trong việc điều hòa chức năng co bóp và tín hiệu phì đại ở các tế bào cơ tim thất. Các kênh Ca2+ loại L kích hoạt giải phóng Ca2+ từ các thụ thể ryanodine để tế bào co bóp, trong khi tín hiệu hạ lưu của các thụ thể liên kết với protein G kích thích giải phóng Ca2+ thông qua các thụ thể inositol 1,4,5-trisphosphate (IP3R), tham gia vào các con đường tín hiệu phì đại. Điều chế biên độ, thời lượng và chu kỳ nhiệm vụ của tín hiệu co bóp Ca2+ trong tế bào chất và định vị không gian đều đã được đề xuất để mã hóa tín hiệu phì đại này. Dựa trên kiến thức hiện tại về IP3R, chúng tôi phát triển một mô hình mô tả hiệu ứng của tương tác chức năng (giao thoa) giữa kênh thụ thể ryanodine và kênh IP3R trên Ca2+ thoáng qua và kiểm tra độ nhạy của hình dạng Ca2+ thoáng qua đối với các đặc tính hoạt hóa IP3R. Một kết quả chính của nghiên cứu của chúng tôi là hoạt hóa IP3R làm tăng thời lượng Ca2+ thoáng qua đối với một loạt các đặc tính IP3R, nhưng hiệu ứng của hoạt hóa IP3R đối với biên độ Ca2+ thoáng qua phụ thuộc vào nồng độ IP3. Hơn nữa, chúng tôi chứng minh rằng giải phóng Ca2+ do IP3 trung gian trong tế bào chất làm tăng chu kỳ nhiệm vụ của Ca2+ thoáng qua, tỷ lệ phần của chu kỳ mà [Ca2+] tăng cao, trên một loạt các giá trị tham số và nồng độ IP3. Khi kết hợp với mô hình hoạt hóa yếu tố phiên mã hạ lưu (NFAT), chúng tôi chứng minh rằng có sự tương ứng cao giữa chu kỳ nhiệm vụ Ca2+ thoáng qua và tỷ lệ NFAT được hoạt hóa trong nhân. Những phát hiện này gợi ý rằng chu kỳ nhiệm vụ Ca2+ trong tế bào chất tăng lên như một cơ chế hợp lý cho tín hiệu phì đại phụ thuộc IP3 thông qua các yếu tố phiên mã nhạy cảm với Ca2+ như NFAT trong các tế bào cơ tim thất."} +{"idx": 1871106, "en": "Breast cancer genome-wide association studies (GWASs) have identified 150 genomic risk regions containing more than 13,000 credible causal variants (CCVs). The CCVs are predominantly noncoding and enriched in regulatory elements. However, the genes underlying breast cancer risk associations are largely unknown. Here, we used genetic colocalization analysis to identify loci at which gene expression could potentially explain breast cancer risk phenotypes. Using data from the Breast Cancer Association Consortium (BCAC) and quantitative trait loci (QTL) from the Genotype-Tissue Expression (GTEx) project and The Cancer Genome Project (TCGA), we identify shared genetic relationships and reveal novel associations between cancer phenotypes and effector genes. Seventeen genes, including NTN4, were identified as potential mediators of breast cancer risk. For NTN4, we showed the rs61938093 CCV at this region was located within an enhancer element that physically interacts with the NTN4 promoter, and the risk allele reduced NTN4 promoter activity. Furthermore, knockdown of NTN4 in breast cells increased cell proliferation in vitro and tumor growth in vivo. These data provide evidence linking risk-associated variation to genes that may contribute to breast cancer predisposition.", "gemini": "Các nghiên cứu về mối liên quan toàn bộ hệ gen về ung thư vú (GWAS) đã xác định 150 vùng nguy cơ trên hệ gen chứa hơn 13.000 biến thể có khả năng gây bệnh (CCV). Các CCV chủ yếu không mã hóa và được làm giàu trong các yếu tố điều hòa. Tuy nhiên, các gen cơ bản liên quan đến nguy cơ ung thư vú phần lớn vẫn chưa được biết. Tại đây, chúng tôi đã sử dụng phân tích định vị gen để xác định các vị trí mà biểu hiện gen có khả năng giải thích các kiểu hình nguy cơ ung thư vú. Sử dụng dữ liệu từ Hiệp hội ung thư vú (BCAC) và các vị trí gen định lượng (QTL) từ dự án Biểu hiện gen mô hình gen (GTEx) và Dự án bộ gen ung thư (TCGA), chúng tôi xác định các mối quan hệ di truyền chung và tiết lộ các mối liên quan mới giữa các kiểu hình ung thư và gen tác động. Mười bảy gen, bao gồm NTN4, đã được xác định là các chất trung gian tiềm ẩn của nguy cơ ung thư vú. Đối với NTN4, chúng tôi chỉ ra rằng CCV rs61938093 tại vùng này nằm trong một yếu tố tăng cường tương tác vật lý với vùng khởi động NTN4 và alen nguy cơ làm giảm hoạt động của vùng khởi động NTN4. Hơn nữa, việc loại bỏ NTN4 ở các tế bào vú làm tăng sự tăng sinh tế bào trong ống nghiệm và sự phát triển khối u trong cơ thể sống. Các dữ liệu này cung cấp bằng chứng liên kết biến thể liên quan đến nguy cơ với các gen có thể góp phần vào khuynh hướng mắc ung thư vú."} +{"idx": 1871107, "en": "Bacterial ribosomal RNAs are synthesized by a dedicated, conserved transcription-elongation complex that transcribes at high rates, shields RNA polymerase from premature termination, and supports co-transcriptional RNA folding, modification, processing, and ribosomal subunit assembly by presently unknown mechanisms. We have determined cryo-electron microscopy structures of complete Escherichia coli ribosomal RNA transcription elongation complexes, comprising RNA polymerase; DNA; RNA bearing an N-utilization-site-like anti-termination element; Nus factors A, B, E, and G; inositol mono-phosphatase SuhB; and ribosomal protein S4. Our structures and structure-informed functional analyses show that fast transcription and anti-termination involve suppression of NusA-stabilized pausing, enhancement of NusG-mediated anti-backtracking, sequestration of the NusG C-terminal domain from termination factor ρ, and the ρ blockade. Strikingly, the factors form a composite RNA chaperone around the RNA polymerase RNA-exit tunnel, which supports co-transcriptional RNA folding and annealing of distal RNA regions. Our work reveals a polymerase/chaperone machine required for biosynthesis of functional ribosomes.", "gemini": "RNA ribosome của vi khuẩn được tổng hợp bởi một phức hợp phiên mã-kéo dài bảo tồn chuyên dụng phiên mã ở tốc độ cao, bảo vệ RNA polymerase khỏi sự kết thúc sớm và hỗ trợ quá trình gấp RNA đồng phiên mã, sửa đổi, xử lý và lắp ráp tiểu đơn vị ribosome bằng các cơ chế hiện chưa biết. Chúng tôi đã xác định cấu trúc hiển vi điện tử lạnh của các phức hợp kéo dài phiên mã RNA ribosome Escherichia coli hoàn chỉnh, bao gồm RNA polymerase; ADN; RNA mang một yếu tố chống kết thúc giống như vị trí sử dụng N; Các yếu tố Nus A, B, E và G; inositol mono-phosphatase SuhB; và protein ribosome S4. Các phân tích chức năng dựa trên cấu trúc và cấu trúc của chúng cho thấy phiên mã nhanh và chống kết thúc liên quan đến việc ức chế tạm dừng ổn định NusA, tăng cường chống lùi do NusG trung gian, cô lập miền đầu cuối C của NusG khỏi yếu tố kết thúc ρ và sự phong tỏa ρ. Đáng ngạc nhiên, các yếu tố tạo thành một chaperone RNA tổng hợp xung quanh đường hầm thoát RNA của RNA polymerase, hỗ trợ quá trình gấp RNA đồng phiên mã và ủ các vùng RNA xa. Công trình của chúng tôi tiết lộ một máy polymerase/chaperone cần thiết cho quá trình sinh tổng hợp ribosome chức năng."} +{"idx": 1871108, "en": "A common approach to benchmarking of single-cell transcriptomics tools is to generate synthetic datasets that statistically resemble experimental data. However, most existing single-cell simulators do not incorporate transcription factor-gene regulatory interactions that underlie expression dynamics. Here, we present SERGIO, a simulator of single-cell gene expression data that models the stochastic nature of transcription as well as regulation of genes by multiple transcription factors according to a user-provided gene regulatory network. SERGIO can simulate any number of cell types in steady state or cells differentiating to multiple fates. We show that datasets generated by SERGIO are statistically comparable to experimental data generated by Illumina HiSeq2000, Drop-seq, Illumina 10X chromium, and Smart-seq. We use SERGIO to benchmark several single-cell analysis tools, including GRN inference methods, and identify Tcf7, Gata3, and Bcl11b as key drivers of T cell differentiation by performing in silico knockout experiments. SERGIO is freely available for download here: https://github.com/PayamDiba/SERGIO.", "gemini": "Một cách tiếp cận phổ biến để đánh giá chuẩn các công cụ phiên mã đơn bào là tạo ra các bộ dữ liệu tổng hợp giống với dữ liệu thực nghiệm về mặt thống kê. Tuy nhiên, hầu hết các trình mô phỏng đơn bào hiện có không kết hợp các tương tác điều hòa gen của yếu tố phiên mã là cơ sở của động lực biểu hiện. Tại đây, chúng tôi trình bày SERGIO, một trình mô phỏng dữ liệu biểu hiện gen đơn bào mô hình bản chất ngẫu nhiên của phiên mã cũng như sự điều hòa của gen bởi nhiều yếu tố phiên mã theo mạng điều hòa gen do người dùng cung cấp. SERGIO có thể mô phỏng bất kỳ số lượng loại tế bào nào ở trạng thái ổn định hoặc các tế bào biệt hóa thành nhiều số phận. Chúng tôi chỉ ra rằng các bộ dữ liệu do SERGIO tạo ra có thể so sánh về mặt thống kê với dữ liệu thực nghiệm do Illumina HiSeq2000, Drop-seq, Illumina 10X chromium và Smart-seq tạo ra. Chúng tôi sử dụng SERGIO để đánh giá chuẩn một số công cụ phân tích đơn bào, bao gồm các phương pháp suy luận GRN, và xác định Tcf7, Gata3 và Bcl11b là những động lực chính của sự biệt hóa tế bào T bằng cách thực hiện các thí nghiệm loại bỏ in silico. SERGIO có thể tải xuống miễn phí tại đây: https://github.com/PayamDiba/SERGIO."} +{"idx": 1871109, "en": "TMPRSS2-ERG gene fusion occurs in approximately 50% of cases of prostate cancer (PCa), and the fusion product is a key driver of prostate oncogenesis. However, how to leverage cellular signaling to ablate TMPRSS2-ERG oncoprotein for PCa treatment remains elusive. Here, we demonstrate that DNA damage induces proteasomal degradation of wild-type ERG and TMPRSS2-ERG oncoprotein through ERG threonine-187 and tyrosine-190 phosphorylation mediated by GSK3β and WEE1, respectively. The dual phosphorylation triggers ERG recognition and degradation by the E3 ubiquitin ligase FBW7 in a manner independent of a canonical degron. DNA damage-induced TMPRSS2-ERG degradation was abolished by cancer-associated PTEN deletion or GSK3β inactivation. Blockade of DNA damage-induced TMPRSS2-ERG oncoprotein degradation causes chemotherapy-resistant growth of fusion-positive PCa cells in culture and in mice. Our findings uncover a previously unrecognized TMPRSS2-ERG protein destruction mechanism and demonstrate that intact PTEN and GSK3β signaling are essential for effective targeting of ERG protein by genotoxic therapeutics in fusion-positive PCa.", "gemini": "Sự hợp nhất gen TMPRSS2-ERG xảy ra ở khoảng 50% các trường hợp ung thư tuyến tiền liệt (PCa) và sản phẩm hợp nhất là động lực chính gây ung thư tuyến tiền liệt. Tuy nhiên, cách tận dụng tín hiệu tế bào để loại bỏ protein ung thư TMPRSS2-ERG để điều trị PCa vẫn còn là điều khó nắm bắt. Ở đây, chúng tôi chứng minh rằng tổn thương DNA gây ra sự thoái hóa proteasome của protein ung thư ERG và TMPRSS2-ERG loại hoang dã thông qua sự phosphoryl hóa threonine-187 và tyrosine-190 của ERG do GSK3β và WEE1 trung gian. Sự phosphoryl hóa kép kích hoạt sự nhận dạng và thoái hóa ERG của ligase ubiquitin E3 FBW7 theo cách không phụ thuộc vào degron điển hình. Sự thoái hóa TMPRSS2-ERG do tổn thương DNA gây ra đã bị xóa bỏ bởi sự mất PTEN liên quan đến ung thư hoặc bất hoạt GSK3β. Việc ngăn chặn sự thoái hóa protein ung thư TMPRSS2-ERG do tổn thương DNA gây ra làm cho các tế bào PCa dương tính với hợp nhất phát triển kháng hóa trị trong nuôi cấy và ở chuột. Những phát hiện của chúng tôi khám phá ra một cơ chế phá hủy protein TMPRSS2-ERG trước đây chưa được biết đến và chứng minh rằng tín hiệu PTEN và GSK3β còn nguyên vẹn là rất cần thiết để nhắm mục tiêu hiệu quả vào protein ERG bằng liệu pháp độc hại đối với gen ở PCa dương tính với hợp nhất."} +{"idx": 1871110, "en": "Epidemiological studies identify midlife hearing loss as an independent risk factor for dementia, estimated to account for 9% of cases. We evaluate candidate brain bases for this relationship. These bases include a common pathology affecting the ascending auditory pathway and multimodal cortex, depletion of cognitive reserve due to an impoverished listening environment, and the occupation of cognitive resources when listening in difficult conditions. We also put forward an alternate mechanism, drawing on new insights into the role of the medial temporal lobe in auditory cognition. In particular, we consider how aberrant activity in the service of auditory pattern analysis, working memory, and object processing may interact with dementia pathology in people with hearing loss. We highlight how the effect of hearing interventions on dementia depends on the specific mechanism and suggest avenues for work at the molecular, neuronal, and systems levels to pin this down.", "gemini": "Các nghiên cứu dịch tễ học xác định mất thính lực ở tuổi trung niên là một yếu tố nguy cơ độc lập đối với chứng mất trí, ước tính chiếm 9% các trường hợp. Chúng tôi đánh giá các cơ sở não bộ ứng viên cho mối quan hệ này. Những cơ sở này bao gồm một bệnh lý chung ảnh hưởng đến đường dẫn thính giác hướng lên và vỏ não đa phương thức, sự suy giảm dự trữ nhận thức do môi trường nghe kém và việc sử dụng các nguồn nhận thức khi nghe trong điều kiện khó khăn. Chúng tôi cũng đưa ra một cơ chế thay thế, dựa trên những hiểu biết mới về vai trò của thùy thái dương giữa trong nhận thức thính giác. Đặc biệt, chúng tôi xem xét cách hoạt động bất thường trong quá trình phân tích mẫu thính giác, trí nhớ làm việc và xử lý đối tượng có thể tương tác với bệnh lý mất trí ở những người bị mất thính lực như thế nào. Chúng tôi nhấn mạnh cách hiệu quả của các biện pháp can thiệp thính giác đối với chứng mất trí phụ thuộc vào cơ chế cụ thể và đề xuất các hướng nghiên cứu ở cấp độ phân tử, nơ-ron và hệ thống để xác định điều này."} +{"idx": 1871111, "en": "Soybean seeds provide a rich source of proteins, fats, carbohydrates, and micronutrients. Extraction and analysis of low abundant soybean seed proteins are challenging because of its complex seed composition. For characterizing various proteins, it is paramount to remove the other interfering components, primarily oils, and carbohydrates. In the present study, we used a sequential dual washing process initially with hexane to remove oil and non-polar interferences, followed by 80% ethanol washing to remove about 60% of the total soluble sugars. The extracted soluble sugars were quantified using a newly developed and validated high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD). This newly developed combined washings process significantly enhanced the separation of both low molecular weight and low abundant proteins using 1D (one dimensional)- and 2D (two dimensional) gel electrophoresis. The separated proteins were trypsinized and analyzed by using Bruker amazon speed ion trap mass spectrometer equipped with an ESI source. This combined washing process allowed the identification of 18 additional low abundant soy proteins as compared to the simple hexane washed samples. This purification process will allow researchers to identify and investigate the role of low molecular weight and low abundant proteins as it relates to plant functions, nutrition, and health.", "gemini": "Hạt đậu nành cung cấp nguồn protein, chất béo, carbohydrate và vi chất dinh dưỡng dồi dào. Việc chiết xuất và phân tích các protein hạt đậu nành có hàm lượng thấp rất khó khăn do thành phần hạt phức tạp của nó. Để xác định đặc điểm của nhiều loại protein khác nhau, điều quan trọng nhất là phải loại bỏ các thành phần gây nhiễu khác, chủ yếu là dầu và carbohydrate. Trong nghiên cứu hiện tại, chúng tôi đã sử dụng quy trình rửa kép tuần tự ban đầu bằng hexan để loại bỏ dầu và các chất gây nhiễu không phân cực, sau đó rửa bằng 80% ethanol để loại bỏ khoảng 60% tổng lượng đường hòa tan. Các loại đường hòa tan được chiết xuất được định lượng bằng máy dò tán xạ ánh sáng bay hơi sắc ký lỏng hiệu suất cao mới được phát triển và xác thực (HPLC-ELSD). Quy trình rửa kết hợp mới được phát triển này đã cải thiện đáng kể việc tách cả protein có trọng lượng phân tử thấp và protein có hàm lượng thấp bằng điện di gel 1D (một chiều) và 2D (hai chiều). Các protein đã tách được được trypsin hóa và phân tích bằng máy quang phổ khối bẫy ion tốc độ Bruker amazon được trang bị nguồn ESI. Quy trình rửa kết hợp này cho phép xác định thêm 18 loại protein đậu nành có hàm lượng thấp so với các mẫu chỉ rửa bằng hexan. Quy trình tinh chế này sẽ cho phép các nhà nghiên cứu xác định và điều tra vai trò của protein có trọng lượng phân tử thấp và hàm lượng thấp liên quan đến chức năng của cây, dinh dưỡng và sức khỏe."} +{"idx": 1871112, "en": "Cardiac arrhythmia is a known manifestation of novel coronavirus 2019 (COVID-19) infection. Herein, we describe the clinical course of an otherwise healthy patient who experienced persistent ventricular tachycardia and fibrillation which is believed to be directly related to inflammation, as opposed to acute myocardial injury or medications that can prolong the QT interval.", "gemini": "Rối loạn nhịp tim là một biểu hiện đã biết của nhiễm trùng virus corona mới 2019 (COVID-19). Trong bài này, chúng tôi mô tả diễn biến lâm sàng của một bệnh nhân khỏe mạnh khác đã trải qua tình trạng nhịp nhanh thất và rung thất dai dẳng được cho là có liên quan trực tiếp đến tình trạng viêm, trái ngược với tổn thương cơ tim cấp tính hoặc các loại thuốc có thể kéo dài khoảng QT."} +{"idx": 1871113, "en": "Acute myocardial infarction (AMI) would sometimes raise severe in-hospital complications such as cardiopulmonary arrest, shock, stroke, atrioventricular block, and respiratory failure. The purpose of this retrospective study was to compare the clinical outcomes of AMI patients who recovered from severe in-hospital complications with those who did not have in-hospital complications. We included 494 AMI patients, and divided those into the in-hospital complications group (n = 166) and noncomplications group (n = 328). The primary end point was the major adverse cardiovascular events (MACE) defined as the composite of all cause death, nonfatal myocardial infarction (MI), and readmission for heart failure within 1 year after the hospital discharge. A total of 50 postdischarge MACE were observed during the study period. MACE was more frequently observed in the in-hospital complications group (14.5%) than in the noncomplications group (7.9%) (p = 0.023). The presence of in-hospital complications was significantly associated with the MACE (Odds Ratio 1.889, 95% Confidence Interval 1.077 to 3.313, p = 0.026) after controlling age, gender, ST-elevation MI, and culprit of AMI. In conclusion, the MACE was significantly frequent in AMI patients who recovered from severe in-hospital complications and discharged to home, as compared with those who did not have in-hospital complications. AMI patients who recovered from complications could be recognized as a high risk group, and should be carefully managed after discharge to prevent cardiovascular events.", "gemini": "Nhồi máu cơ tim cấp (AMI) đôi khi gây ra các biến chứng nghiêm trọng trong bệnh viện như ngừng tim phổi, sốc, đột quỵ, block nhĩ thất và suy hô hấp. Mục đích của nghiên cứu hồi cứu này là so sánh kết quả lâm sàng của bệnh nhân AMI hồi phục sau biến chứng nặng trong bệnh viện với những bệnh nhân không có biến chứng trong bệnh viện. Chúng tôi đã đưa vào nghiên cứu 494 bệnh nhân AMI và chia thành nhóm biến chứng trong bệnh viện (n = 166) và nhóm không biến chứng (n = 328). Điểm cuối chính là các biến cố tim mạch bất lợi lớn (MACE) được định nghĩa là hợp thành của tất cả các nguyên nhân tử vong, nhồi máu cơ tim không tử vong (MI) và nhập viện trở lại vì suy tim trong vòng 1 năm sau khi xuất viện. Tổng cộng 50 MACE sau xuất viện đã được quan sát trong thời gian nghiên cứu. MACE được quan sát thấy thường xuyên hơn ở nhóm biến chứng trong bệnh viện (14,5%) so với nhóm không biến chứng (7,9%) (p = 0,023). Sự có mặt của các biến chứng trong bệnh viện có liên quan đáng kể đến MACE (Tỷ lệ cược 1,889, Khoảng tin cậy 95% 1,077 đến 3,313, p = 0,026) sau khi kiểm soát tuổi, giới tính, MI ST chênh lệch và nguyên nhân gây AMI. Tóm lại, MACE thường xuyên xảy ra ở những bệnh nhân AMI hồi phục sau biến chứng nặng trong bệnh viện và xuất viện về nhà, so với những bệnh nhân không có biến chứng trong bệnh viện. Bệnh nhân AMI hồi phục sau biến chứng có thể được coi là nhóm có nguy cơ cao và cần được quản lý cẩn thận sau khi xuất viện để ngăn ngừa các biến cố tim mạch."} +{"idx": 1871114, "en": "Dextromethorphan (DM) is a cough suppressant available in many prescribed and over-the-counter medications. Adverse reactions induced by DM have been regularly reported, including allergic skin reactions in some cases. However, the underlying mechanisms of local anaphylaxis induced by DM have not been elucidated. In this study, we found that DM could activate mast cells to increase calcium mobilization and release β-hexosaminidase, histamine, tumor necrosis factor-α, MCP-1, and IL-8 in a dose-dependent manner. The allergic reactions were confirmed by hind paw swelling and extravasation assay in vivo. Furthermore, DM was revealed to induce local anaphylaxis via MRGPRX2 by the mast cell-deficient kitW-sh/W-sh mice and MRGPRX2 knockdown mast cells. And the MRGPRX2-HEK293/CMC analysis and frontal analysis also showed that DM has a considerable affinity with MRGPRX2. Together, our findings suggest that close monitoring should be drawn on patients with DM for its potential anaphylaxis via MRGPRX2.", "gemini": "Dextromethorphan (DM) là thuốc giảm ho có trong nhiều loại thuốc theo toa và không kê đơn. Các phản ứng có hại do DM gây ra đã được báo cáo thường xuyên, bao gồm cả các phản ứng dị ứng trên da trong một số trường hợp. Tuy nhiên, cơ chế gây ra phản vệ tại chỗ do DM gây ra vẫn chưa được làm sáng tỏ. Trong nghiên cứu này, chúng tôi phát hiện ra rằng DM có thể kích hoạt tế bào mast để tăng động viên canxi và giải phóng β-hexosaminidase, histamine, yếu tố hoại tử khối u-α, MCP-1 và IL-8 theo cách phụ thuộc vào liều. Các phản ứng dị ứng đã được xác nhận bằng cách sưng bàn chân sau và xét nghiệm thoát mạch trong ống nghiệm. Hơn nữa, DM được tiết lộ là gây ra phản vệ tại chỗ thông qua MRGPRX2 bởi chuột mast tế bào thiếu kitW-sh/W-sh và tế bào mast loại bỏ MRGPRX2. Và phân tích MRGPRX2-HEK293/CMC và phân tích mặt trước cũng cho thấy DM có ái lực đáng kể với MRGPRX2. Kết hợp lại, những phát hiện của chúng tôi cho thấy nên theo dõi chặt chẽ những bệnh nhân dùng DM vì khả năng gây phản vệ thông qua MRGPRX2."} +{"idx": 1871115, "en": "The ADP/ATP carrier (AAC) transports matrix ATP and cytosolic ADP across the inner mitochondrial membrane (IMM). It is well known that cardiolipin (CL) plays an important role in regulating the function of AAC, yet the underlying mechanism still remains elusive. AAC is composed of three homologous domains, and three specific CL binding sites are located at the domain-domain interfaces near the matrix side. Here we report an in-depth investigation on the dynamic properties of the bound CL within the three specific sites through all-atom molecular dynamics simulations of up to 13 μs in total. Our results highlight the importance of the basic and polar residues in CL binding. The basic residues from the linker helix and/or the [Y/W/F][K/R]G motif enable the bound CL to form an intra-domain binding mode, and the canonical inter-domain binding mode only forms when these basic residues are occupied by an additional phospholipid. Of special significance, differences in the basic and polar residues lead to remarkable asymmetry among the three specific CL binding sites. We found that the bound CL at the interface of domains 2 and 3 predominantly adopts inter-domain binding mode, while CLs at the other two sites have much more intra-domain populations. This is consistent with the asymmetric crystal structure of the matrix state (m-state) AAC which implies an asymmetric transport mechanism. The dynamic equilibrium between the inter-domain and intra-domain binding modes observed in our simulations could be highly important for the bound CLs to adapt to the movements during state transitions.", "gemini": "Chất vận chuyển ADP/ATP (AAC) vận chuyển ATP ma trận và ADP tế bào chất qua màng ty thể bên trong (IMM). Người ta biết rằng cardiolipin (CL) đóng vai trò quan trọng trong việc điều chỉnh chức năng của AAC, nhưng cơ chế cơ bản vẫn còn khó nắm bắt. AAC được cấu tạo từ ba miền đồng dạng và ba vị trí liên kết CL cụ thể nằm ở các giao diện miền-miền gần phía ma trận. Ở đây, chúng tôi báo cáo một cuộc điều tra chuyên sâu về các tính chất động của CL liên kết trong ba vị trí cụ thể thông qua các mô phỏng động lực học phân tử toàn nguyên tử lên đến 13 μs tổng cộng. Kết quả của chúng tôi làm nổi bật tầm quan trọng của các gốc bazơ và phân cực trong liên kết CL. Các gốc bazơ từ xoắn ốc liên kết và/hoặc mô típ [Y/W/F][K/R]G cho phép CL liên kết tạo thành chế độ liên kết trong miền và chế độ liên kết giữa các miền điển hình chỉ hình thành khi các gốc bazơ này bị chiếm bởi một phospholipid bổ sung. Đặc biệt quan trọng, sự khác biệt về các gốc bazơ và phân cực dẫn đến sự không đối xứng đáng kể giữa ba vị trí liên kết CL cụ thể. Chúng tôi thấy rằng CL liên kết tại giao diện của miền 2 và 3 chủ yếu áp dụng chế độ liên kết giữa các miền, trong khi CL ở hai vị trí còn lại có nhiều quần thể trong miền hơn. Điều này phù hợp với cấu trúc tinh thể không đối xứng của trạng thái ma trận (trạng thái m) của AAC, ngụ ý một cơ chế vận chuyển không đối xứng. Sự cân bằng động giữa các chế độ liên kết giữa các miền và trong miền được quan sát thấy trong các mô phỏng của chúng tôi có thể rất quan trọng để các CL liên kết thích ứng với các chuyển động trong quá trình chuyển đổi trạng thái."} +{"idx": 1871116, "en": "Antimicrobial peptides (AMPs) are effective alternatives to conventional antibiotics. They protect the host from the constant invasion of a broad range of infectious microorganisms. AMPs have been at the forefront of the response to multidrug-resistant microbial strains and appear to be ideal drug candidates. Lactophoricin (LPcin), naturally produced from bovine milk, is a typical cationic antimicrobial peptide. Three analog peptides, including LPcin-YK5, LPcin-YK8, and LPcin-YK11, with enhanced antimicrobial activity compared to the wild-type LPcin, were designed and expressed in our laboratory. We investigated the structure and antimicrobial mechanisms of action of the three novel antimicrobial peptide analogs derived from LPcin using solution NMR and solid-state NMR spectroscopy in membrane environments. Our results revealed that the three LPcin analogs exhibited helical structures with different tilt angles on the phospholipid membrane surface. We proposed three-dimensional conformations and antibacterial mechanisms of action of the three peptide analogs in the phospholipid bilayers using two-dimensional solid-state separated local field NMR experiments.", "gemini": "Các peptit kháng khuẩn (AMP) là những lựa chọn thay thế hiệu quả cho các loại kháng sinh thông thường. Chúng bảo vệ vật chủ khỏi sự xâm nhập liên tục của nhiều loại vi sinh vật gây bệnh. Các AMP đã đi đầu trong phản ứng với các chủng vi khuẩn kháng nhiều loại thuốc và dường như là các ứng cử viên lý tưởng cho thuốc. Lactophoricin (LPcin), được sản xuất tự nhiên từ sữa bò, là một peptit kháng khuẩn cation điển hình. Ba peptit tương tự, bao gồm LPcin-YK5, LPcin-YK8 và LPcin-YK11, có hoạt tính kháng khuẩn tăng cường so với LPcin dạng hoang dã, đã được thiết kế và biểu hiện trong phòng thí nghiệm của chúng tôi. Chúng tôi đã nghiên cứu cấu trúc và cơ chế kháng khuẩn của ba tương tự peptit kháng khuẩn mới có nguồn gốc từ LPcin bằng cách sử dụng phổ NMR dung dịch và phổ NMR trạng thái rắn trong môi trường màng. Kết quả của chúng tôi cho thấy ba tương tự LPcin biểu hiện các cấu trúc xoắn ốc với các góc nghiêng khác nhau trên bề mặt màng phospholipid. Chúng tôi đã đề xuất các cấu hình ba chiều và cơ chế tác dụng diệt khuẩn của ba tương tự peptit trong các lớp kép phospholipid bằng cách sử dụng các thí nghiệm NMR trường cục bộ tách biệt trạng thái rắn hai chiều."} +{"idx": 1871117, "en": "Sphingolipids-enriched rafts domains are proposed to occur in plasma membranes and to mediate important cellular functions. Notwithstanding, the asymmetric transbilayer distribution of phospholipids that exists in the membrane confers the two leaflets different potentials to form lateral domains as next to no sphingolipids are present in the inner leaflet. How the physical properties of one leaflet can influence the properties of the other and its importance on signal transduction across the membrane are questions still unresolved. In this work, we combined AFM imaging and Force spectroscopy measurements to assess domain formation and to study the nanomechanical properties of asymmetric supported lipid bilayers (SLBs) mimicking membrane rafts. Asymmetric SLBs were formed by incorporating N-palmitoyl-sphingomyelin (16:0SM) into the outer leaflet of preformed 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC)/Cholesterol SLBs through methyl-β-cyclodextrin-mediated lipid exchange. Lipid domains were detected after incorporation of 16:0SM though their phase state varied from gel to liquid ordered (Lo) phase if the procedure was performed at 24 or 37 °C, respectively. When comparing symmetric and asymmetric Lo domains, differences in size and morphology were observed, with asymmetric domains being smaller and more interconnected. Both types of Lo domains showed similar mechanical stability in terms of rupture forces and Young's moduli. Notably, force curves in asymmetric domains presented two rupture events that could be attributed to the sequential rupture of a liquid disordered (Ld) and a Lo phase. Interleaflet coupling in asymmetric Lo domains could also be inferred from those measurements. The experimental approach outlined here would significantly enhance the applicability of membrane models.", "gemini": "Các miền bè giàu sphingolipid được cho là xuất hiện trong màng plasma và có chức năng trung gian cho các chức năng quan trọng của tế bào. Mặc dù vậy, sự phân bố lớp kép xuyên màng không đối xứng của phospholipid tồn tại trong màng mang lại cho hai tờ giấy tiềm năng khác nhau để tạo thành các miền bên vì hầu như không có sphingolipid nào có mặt ở tờ giấy bên trong. Làm thế nào mà các tính chất vật lý của một tờ giấy có thể ảnh hưởng đến các tính chất của tờ giấy kia và tầm quan trọng của nó đối với quá trình truyền tín hiệu qua màng vẫn là những câu hỏi chưa được giải quyết. Trong nghiên cứu này, chúng tôi đã kết hợp hình ảnh AFM và phép đo phổ lực để đánh giá sự hình thành miền và nghiên cứu các tính chất cơ học nano của các lớp kép lipid được hỗ trợ không đối xứng (SLB) bắt chước bè màng. Các SLB không đối xứng được hình thành bằng cách kết hợp N-palmitoyl-sphingomyelin (16:0SM) vào tờ giấy bên ngoài của các SLB 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC)/Cholesterol được tạo sẵn thông qua trao đổi lipid được trung gian bởi methyl-β-cyclodextrin. Các miền lipid được phát hiện sau khi kết hợp 16:0SM mặc dù trạng thái pha của chúng thay đổi từ pha gel sang pha lỏng có trật tự (Lo) nếu quy trình được thực hiện ở 24 hoặc 37 °C. Khi so sánh các miền Lo đối xứng và không đối xứng, người ta quan sát thấy sự khác biệt về kích thước và hình thái, với các miền không đối xứng nhỏ hơn và có nhiều liên kết hơn. Cả hai loại miền Lo đều cho thấy độ ổn định cơ học tương tự về lực đứt gãy và mô đun Young. Đáng chú ý, các đường cong lực trong các miền không đối xứng thể hiện hai sự kiện đứt gãy có thể được quy cho sự đứt gãy tuần tự của pha lỏng không trật tự (Ld) và pha Lo. Sự liên kết giữa các tờ giấy trong các miền Lo không đối xứng cũng có thể được suy ra từ các phép đo đó. Phương pháp tiếp cận thực nghiệm được nêu ở đây sẽ tăng cường đáng kể khả năng áp dụng của các mô hình màng."} +{"idx": 1871118, "en": "Indoleamine-2,3-dioxygenase 1 (IDO1) and tryptophan-2,3-dioxygenase 2 (TDO2) degrade tryptophan (Trp) to kynurenine (Kyn), and these enzymes have promise as therapeutic targets. A comprehensive characterization of potential safety liabilities of IDO1 and TDO2 inhibitors using knockout (KO) mice has not been assessed, nor has the dual Ido1/Tdo2 KO been reported. Here we characterized male and female mice with KOs for Ido1, Tdo2, and Ido1/Tdo2 and compared findings to the wild type (WT) mouse strain, evaluated for 14 days, using metabolomics, transcriptional profiling, behavioral analysis, spleen immunophenotyping, comprehensive histopathological analysis, and serum clinical chemistry. Multiple metabolomic changes were seen in KO mice. For catabolism of Trp to Kyn and anthranilic acid, both substrates were decreased in liver of Tdo2 and dual KO mice. Metabolism of Trp to serotonin and its metabolites resulted in an increase in 5-Hydroxyindole-3-acetic acid in the Tdo2 and dual KO mice. Ido1 and dual KO mice displayed a Kyn reduction in plasma but not in liver. Nicotinamide synthesis and conversion of glucose to lactic acid were not impacted. A slight decrease in serum alkaline phosphatase was seen in all KOs, and small changes in liver gene expression of genes unrelated to tryptophan metabolism were observed. Regarding other parameters, no genotype-specific changes were observed. In summary, this work shows metabolomic pathway changes for metabolites downstream of tryptophan in these KO mice, and suggests that inhibition of the IDO1 and TDO2 enzymes would be well tolerated whether inhibited individually or in combination since no safety liabilities were uncovered.", "gemini": "Indoleamine-2,3-dioxygenase 1 (IDO1) và tryptophan-2,3-dioxygenase 2 (TDO2) phân hủy tryptophan (Trp) thành kynurenine (Kyn) và các enzym này hứa hẹn là mục tiêu điều trị. Một đặc điểm toàn diện về các nguy cơ tiềm ẩn về an toàn của các chất ức chế IDO1 và TDO2 sử dụng chuột loại trừ (KO) vẫn chưa được đánh giá và cũng chưa có báo cáo về loại trừ Ido1/Tdo2 kép. Ở đây, chúng tôi mô tả đặc điểm của chuột đực và cái bị loại trừ Ido1, Tdo2 và Ido1/Tdo2 và so sánh các phát hiện với chủng chuột kiểu hoang dã (WT), được đánh giá trong 14 ngày, sử dụng các phương pháp nghiên cứu chuyển hóa, lập hồ sơ phiên mã, phân tích hành vi, miễn dịch kiểu lách, phân tích mô bệnh học toàn diện và hóa học lâm sàng huyết thanh. Nhiều thay đổi chuyển hóa được thấy ở chuột KO. Đối với quá trình dị hóa Trp thành Kyn và axit anthranilic, cả hai chất nền đều giảm ở gan của chuột Tdo2 và chuột KO kép. Quá trình chuyển hóa Trp thành serotonin và các chất chuyển hóa của nó dẫn đến sự gia tăng axit 5-Hydroxyindole-3-acetic ở chuột Tdo2 và chuột KO kép. Chuột Ido1 và chuột KO kép biểu hiện sự giảm Kyn trong huyết tương nhưng không có trong gan. Sự tổng hợp nicotinamide và quá trình chuyển đổi glucose thành axit lactic không bị ảnh hưởng. Một sự giảm nhẹ phosphatase kiềm trong huyết thanh được thấy ở tất cả các loại KO và những thay đổi nhỏ trong biểu hiện gen gan của các gen không liên quan đến quá trình chuyển hóa tryptophan đã được quan sát. Đối với các thông số khác, không có thay đổi đặc hiệu về kiểu gen nào được quan sát. Tóm lại, nghiên cứu này cho thấy những thay đổi về con đường chuyển hóa đối với các chất chuyển hóa hạ nguồn của tryptophan ở những con chuột KO này và cho thấy rằng việc ức chế các enzym IDO1 và TDO2 sẽ được dung nạp tốt cho dù ức chế riêng lẻ hay kết hợp vì không phát hiện thấy nguy cơ an toàn nào."} +{"idx": 1871119, "en": "A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns. Basic monosaccharide composition indicated that SSIPS1 was mainly composed of d-glucose. The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked α-d-glucopyranose (Glcp) residues with two branched points at O-6. The two branches were composed of 1,4-linked α-D-Glcp terminated with α-D-Glcp, 1,4-linked α-D-Glcp and 1,4-linked β-Galp terminated by α-D-Glcp. Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy. Further, SSIPS1 exhibited a potential inhibitory activity against α-amylase and α-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well.", "gemini": "Một polysaccharide trung tính (SSIPS1) đã được phân lập và tinh chế từ sợi nấm nuôi cấy của Sanghuangporus sanghuang bằng cột sắc ký DEAE Sepharose Fast Flow và Sephacryl S-100. Thành phần monosaccharide cơ bản cho thấy SSIPS1 chủ yếu bao gồm d-glucose. Kết quả phân tích metyl hóa và 2D-NMR cho thấy các liên kết glycosid của SSIPS1 được làm sáng tỏ là bao gồm các gốc α-d-glucopyranose (Glcp) liên kết 1,4 với hai điểm nhánh tại O-6. Hai nhánh này bao gồm α-D-Glcp liên kết 1,4 kết thúc bằng α-D-Glcp, α-D-Glcp liên kết 1,4 và β-Galp liên kết 1,4 kết thúc bằng α-D-Glcp. Hơn nữa, cấu hình chuỗi của nó được tiết lộ là có cấu hình chuỗi linh hoạt trong 0,1 NaNO3 với bán kính thủy động lực học và bán kính hồi chuyển là 3,26 và 6,45 nm bằng phương pháp tán xạ ánh sáng laser nhiều góc, với một chuỗi đơn 0,559 nm quan sát được bằng kính hiển vi lực nguyên tử. Hơn nữa, SSIPS1 thể hiện hoạt tính ức chế tiềm tàng đối với α-amylase và α-glucosidase, và nó cũng có tác dụng hạ đường huyết đối với tình trạng kháng insulin trong ống nghiệm của tế bào HepG2."} +{"idx": 1871120, "en": "The bioactive form of thermostable and alkali stable pectinase of Bacillus pumilus dcsr1 is a homodimer of the molecular mass of 60 kDa with a pI of 4.6. The enzyme is optimally active at 50 °C and pH 10.5, and its Michaelis constant (Km), maximum rate of reaction (Vmax), activation energy (Ea), and temperature quotient (Q10) values (for citrus pectin) are 0.29 mg mL-1, 116 μmole mg-1 min-1, 74.73 KJmol-1 and 1.57, respectively. The enzyme has a shelf life of one and a half years at room temperature as well as 4 °C. The activity of the enzyme is stimulated by Mn2+ and Ca2+ and inhibited by Hg+, Cd2+, Co2+, Zn2+, Fe2+, Pb2+, EDTA and urea to a varied extent. The conformational studies of the enzyme revealed a high β-sheet content in the bioactive dimer, and high α-helix in the inactive monomer. The Circular Dichroism (CD) spectra of the dimer in the presence of inhibitors suggested a marked decrease in β-sheet, and a significant increase in α-helix, suggesting a key role of β-sheets in the enzyme catalysis. Based on the end product analysis, the enzyme is an exopolygalacturonase with a unique ability of transglycosylation. When ramie fibers were treated with the enzyme, removal of gummy material (pectin) was visible, confirming its applicability in the degumming process.", "gemini": "Dạng hoạt tính sinh học của pectinase bền nhiệt và bền kiềm của Bacillus pumilus dcsr1 là một homodimer có khối lượng phân tử 60 kDa với pI là 4,6. Enzym hoạt động tối ưu ở 50 °C và pH 10,5, và hằng số Michaelis (Km), tốc độ phản ứng tối đa (Vmax), năng lượng hoạt hóa (Ea) và giá trị thương số nhiệt độ (Q10) (đối với pectin cam quýt) lần lượt là 0,29 mg mL-1, 116 μmol mg-1 phút-1, 74,73 KJmol-1 và 1,57. Enzym có thời hạn sử dụng là một năm rưỡi ở nhiệt độ phòng cũng như 4 °C. Hoạt động của enzym được kích thích bởi Mn2+ và Ca2+ và bị ức chế bởi Hg+, Cd2+, Co2+, Zn2+, Fe2+, Pb2+, EDTA và urê ở các mức độ khác nhau. Các nghiên cứu về cấu hình của enzyme cho thấy hàm lượng β-sheet cao trong dimer hoạt tính sinh học và hàm lượng α-helix cao trong monomer không hoạt động. Phổ CD (Circular Dichroism) của dimer có mặt chất ức chế cho thấy sự giảm rõ rệt của β-sheet và sự gia tăng đáng kể của α-helix, cho thấy vai trò quan trọng của β-sheet trong xúc tác của enzyme. Dựa trên phân tích sản phẩm cuối cùng, enzyme là một exopolygalacturonase có khả năng chuyển glycosyl hóa độc đáo. Khi sợi đay được xử lý bằng enzyme, có thể thấy rõ sự loại bỏ vật liệu dạng keo (pectin), xác nhận khả năng ứng dụng của nó trong quá trình khử keo."} +{"idx": 1871121, "en": "Golgi Reassembly and Stacking Proteins (GRASPs) were firstly described as crucial elements in determining the structure of the Golgi complex. However, data have been accumulating over the years showing GRASPs can participate in various cell processes beyond the Golgi maintenance, including cell adhesion and migration, autophagy and unconventional secretion of proteins. A comprehensive understanding of the GRASP functions requires deep mechanistic knowledge of its structure and dynamics, especially because of the unique structural plasticity observed for many members of this family coupled with their high promiscuity in mediating protein-protein interactions. Here, we critically review data regarding the structural biophysics of GRASPs in the quest for understanding the structural determinants of different functionalities. We dissect GRASP structure starting with the full-length protein down to its separate domains (PDZ1, PDZ2 and SPR) and outline some structural features common to all members of the GRASP family (such as the presence of many intrinsically disordered regions). Although the impact of those exquisite properties in vivo will still require further studies, it is possible, from our review, to pinpoint factors that must be considered in future interpretation of data regarding GRASP functions, thus bringing somewhat new perspectives to the field.", "gemini": "Các protein lắp ráp và xếp chồng Golgi (GRASP) được mô tả lần đầu tiên là các yếu tố quan trọng trong việc xác định cấu trúc của phức hợp Golgi. Tuy nhiên, dữ liệu đã tích lũy trong nhiều năm cho thấy GRASP có thể tham gia vào nhiều quá trình tế bào ngoài việc duy trì Golgi, bao gồm cả sự kết dính và di chuyển tế bào, autophagy và tiết protein không theo quy ước. Để hiểu toàn diện về chức năng của GRASP đòi hỏi phải có kiến thức sâu sắc về cơ chế cấu trúc và động lực của nó, đặc biệt là do tính dẻo cấu trúc độc đáo được quan sát thấy ở nhiều thành viên của họ này kết hợp với sự hỗn tạp cao của chúng trong việc trung gian tương tác protein-protein. Ở đây, chúng tôi xem xét một cách nghiêm ngặt dữ liệu liên quan đến sinh lý học cấu trúc của GRASP trong quá trình tìm kiếm để hiểu các yếu tố quyết định cấu trúc của các chức năng khác nhau. Chúng tôi phân tích cấu trúc GRASP bắt đầu từ protein toàn phần cho đến các miền riêng biệt của nó (PDZ1, PDZ2 và SPR) và phác thảo một số đặc điểm cấu trúc chung cho tất cả các thành viên của họ GRASP (chẳng hạn như sự hiện diện của nhiều vùng không theo trật tự nội tại). Mặc dù tác động của những tính chất tinh tế đó trong cơ thể sống vẫn cần được nghiên cứu thêm, nhưng từ bài đánh giá của chúng tôi, có thể xác định các yếu tố phải được xem xét trong việc giải thích dữ liệu trong tương lai liên quan đến chức năng của GRASP, do đó mang lại những góc nhìn mới cho lĩnh vực này."} +{"idx": 1871122, "en": "Bites evoked by Brown spiders (Loxosceles genus) are associated with skin injuries (cutaneous rash, itching, swelling, erythema and dermonecrosis) and systemic manifestations. Transcriptome analyses of Loxosceles venom glands showed that the venom has a complex composition containing toxins such as phospholipases-D, metalloproteases and hyaluronidases. Here, by screening the RNA from L. intermedia venom glands, we cloned a novel allergen toxin, and named LALLT (LoxoscelesAllergen-Like Toxin). Sequence analysis showed that LALLT is closely related to allergens from other spiders and RNA screening indicated the presence of LALLT orthologues in the venom of other Loxosceles spiders. Recombinant LALLT was expressed (~45 kDa) in baculovirus-infected insect cells and purified by affinity chromatography. Antibodies against different Loxosceles venoms cross-reacted with LALLT and antibodies against LALLT recognized three Loxosceles venoms, revealing epitopes identity. LALLT triggered paw edema in mice and erythema, edema and leukocyte infiltration into the dermis of rabbit skin. Also, LALLT induced vascular permeability in mice, degranulation of rat mesentery mast cells, as well as prompted degranulation and increased calcium influx in RBL-2H3 cells. Data reported suggest for the first time the existence of allergens in Loxosceles venoms and make LALLT available for clinical studies about allergenic events arisen by Loxosceles envenoming.", "gemini": "Các vết cắn do nhện nâu (chi Loxosceles) gây ra liên quan đến các tổn thương da (phát ban trên da, ngứa, sưng, ban đỏ và hoại tử da) và các biểu hiện toàn thân. Các phân tích phiên mã của tuyến nọc Loxosceles cho thấy nọc có thành phần phức tạp chứa các độc tố như phospholipase-D, metalloprotease và hyaluronidase. Ở đây, bằng cách sàng lọc RNA từ các tuyến nọc L. intermedia, chúng tôi đã nhân bản được một độc tố gây dị ứng mới và đặt tên là LALLT (LoxoscelesAllergen-Like Toxin). Phân tích trình tự cho thấy LALLT có liên quan chặt chẽ đến các chất gây dị ứng từ các loài nhện khác và sàng lọc RNA cho thấy sự hiện diện của các ortholog LALLT trong nọc của các loài nhện Loxosceles khác. LALLT tái tổ hợp được biểu hiện (~45 kDa) trong các tế bào côn trùng bị nhiễm vi-rút cầu gai và được tinh chế bằng sắc ký ái lực. Các kháng thể chống lại các nọc Loxosceles khác nhau phản ứng chéo với LALLT và các kháng thể chống lại LALLT nhận ra ba nọc Loxosceles, cho thấy bản sắc epitop. LALLT gây phù bàn chân ở chuột và ban đỏ, phù nề và thâm nhiễm bạch cầu vào lớp hạ bì của da thỏ. Ngoài ra, LALLT gây ra tính thấm mạch ở chuột, giải phóng các tế bào mast mạc treo của chuột, cũng như thúc đẩy giải phóng và tăng dòng calci trong các tế bào RBL-2H3. Dữ liệu được báo cáo cho thấy lần đầu tiên sự tồn tại của các chất gây dị ứng trong nọc Loxosceles và làm cho LALLT có sẵn cho các nghiên cứu lâm sàng về các sự kiện gây dị ứng phát sinh do ngộ độc Loxosceles."} +{"idx": 1871123, "en": "Antifungal proteins (AFPs) offer a great potential as new biofungicides to control deleterious fungi. The phytopathogenic fungus Penicillium expansum encodes three phylogenetically distinct AFPs, PeAfpA, PeAfpB and PeAfpC. Here, PeAfpA, a potent in vitro self-inhibitory protein, was demonstrated to control the infection caused by P. expansum in Golden apple fruits. We determined the production of the three proteins in different growth media. PeAfpA and PeAfpC were simultaneously produced by P. expansum in three out of the eight media tested as detected by Western blot, whereas PeAfpB was not detected even in those described for class B AFP production. Regardless of the culture medium, the carbon source affected Peafp expression. Notably, the production of PeAfpA was strain-dependent, but analyses of PeafpA regulatory sequences in the three strains studied could not explain differences in protein production. None of the PeAFPs was produced during apple infection, suggesting no relevant role in pathogenesis. PeAfpA together with PeAfpB and also with Penicillium digitatum PdAfpB showed synergistic interaction. The highly active antifungal PeAfpA also showed moderate antibacterial activity. We conclude that there is not a general pattern for Peafp gene expression, protein production or antimicrobial activity and confirm PeAfpA as a promising compound for postharvest conservation.", "gemini": "Các protein kháng nấm (AFP) có tiềm năng rất lớn như các chất diệt nấm sinh học mới để kiểm soát các loại nấm có hại. Nấm gây bệnh thực vật Penicillium expansum mã hóa ba AFP khác biệt về mặt phát sinh loài, PeAfpA, PeAfpB và PeAfpC. Ở đây, PeAfpA, một protein tự ức chế mạnh trong ống nghiệm, đã được chứng minh là kiểm soát nhiễm trùng do P. expansum gây ra ở quả táo Golden. Chúng tôi xác định việc sản xuất ba protein trong các môi trường tăng trưởng khác nhau. PeAfpA và PeAfpC được P. expansum sản xuất đồng thời trong ba trong số tám môi trường được thử nghiệm khi phát hiện bằng phương pháp Western blot, trong khi PeAfpB không được phát hiện ngay cả trong những môi trường được mô tả để sản xuất AFP loại B. Bất kể môi trường nuôi cấy nào, nguồn cacbon đều ảnh hưởng đến biểu hiện Peafp. Đáng chú ý, việc sản xuất PeAfpA phụ thuộc vào chủng, nhưng các phân tích trình tự điều hòa PeafpA trong ba chủng được nghiên cứu không thể giải thích được sự khác biệt trong sản xuất protein. Không có PeAFP nào được sản xuất trong quá trình nhiễm táo, cho thấy không có vai trò liên quan trong sinh bệnh. PeAfpA cùng với PeAfpB và cũng với Penicillium digitatum PdAfpB cho thấy sự tương tác hiệp đồng. PeAfpA kháng nấm có hoạt tính cao cũng cho thấy hoạt tính kháng khuẩn vừa phải. Chúng tôi kết luận rằng không có mô hình chung nào cho biểu hiện gen Peafp, sản xuất protein hoặc hoạt tính kháng khuẩn và xác nhận PeAfpA là một hợp chất đầy hứa hẹn để bảo quản sau thu hoạch."} +{"idx": 1871124, "en": "Chondroitinases degrade chondroitin sulfate (CS) into oligosaccharides, of which the biological activities have vital roles in various fields. Some chondroitinases in polysaccharide lyase family 8 (PL8) have been classified into four subfamilies (PL8_1, PL8_2, PL8_3, and PL8_4) based on their sequence similarity and substrate specificities. In this study, a gene, vpa_0049, was cloned from marine bacterium Vibrio sp. QY108. The encoded protein, Vpa_0049, did not belong to the four existing subfamilies in PL8 based on phylogenetic analysis. Vpa_0049 could degrade various glycosaminoglycans (CS-A, CS-B, CS-C, CS-D, and HA) into unsaturated disaccharides in an endolytic manner, which was different from PL8 lyases of four existing subfamilies. The maximum activity of Vpa_0049 on different glycosaminoglycan substrates appeared at 30-37 °C and pH 7.0-8.0 in the presence of NaCl. Vpa_0049 showed approximately 50% of maximum activity towards CS-B and HA at 0 °C. It was stable in alkaline conditions (pH 8.0-10.6) and 0-30 °C. Our study provides a new broad-substrate chondroitinase and presents an in-depth understanding of PL8.", "gemini": "Chondroitinase phân hủy chondroitin sulfate (CS) thành oligosacarit, trong đó các hoạt tính sinh học đóng vai trò quan trọng trong nhiều lĩnh vực. Một số chondroitinase trong họ polysaccharide lyase 8 (PL8) đã được phân loại thành bốn phân họ (PL8_1, PL8_2, PL8_3 và PL8_4) dựa trên sự giống nhau về trình tự và tính đặc hiệu của cơ chất. Trong nghiên cứu này, một gen, vpa_0049, đã được nhân bản từ vi khuẩn biển Vibrio sp. QY108. Protein được mã hóa, Vpa_0049, không thuộc về bốn phân họ hiện có trong PL8 dựa trên phân tích phát sinh loài. Vpa_0049 có thể phân hủy nhiều glycosaminoglycan khác nhau (CS-A, CS-B, CS-C, CS-D và HA) thành disacarit không bão hòa theo cách nội phân, khác với lyase PL8 của bốn phân họ hiện có. Hoạt động tối đa của Vpa_0049 trên các cơ chất glycosaminoglycan khác nhau xuất hiện ở 30-37 °C và pH 7,0-8,0 khi có mặt NaCl. Vpa_0049 cho thấy khoảng 50% hoạt động tối đa đối với CS-B và HA ở 0 °C. Nó ổn định trong điều kiện kiềm (pH 8,0-10,6) và 0-30 °C. Nghiên cứu của chúng tôi cung cấp một chondroitinase có cơ chất rộng mới và trình bày hiểu biết sâu sắc về PL8."} +{"idx": 1871125, "en": "The present paper describes the synthesis of a novel magnetic chitosan (CCF), in which the carbon-Fe3O4 core-shell nanoparticles play the role of magnetic part. The structure, property and morphology of the magnetic CCF were characterized by FT-IR, XRD, EDAX, SEM and BET techniques. Its adsorption performance was investigated for the removal of methyl orange from aqueous solutions by varying experimental conditions. The results showed the fast adsorption of methyl orange in wide pH range of 3-11 and the maximum adsorption capacity was found to be 425 mg g-1 at 45 °C. The results of adsorption kinetics indicated that the adsorption mechanism was better described by the pseudo-second-order equation, whereas pore diffusion is the rate-controlling of adsorption kinetics. Furthermore, among different isotherm models, Langmuir and Sips isotherm models fitted well the equilibrium experimental data at different temperatures revealing the surface heterogeneity of the adsorbents. The adsorbent exhibited high adsorption performance, compared to the some other chitosan adsorbents reported in literatures.", "gemini": "Bài báo hiện tại mô tả quá trình tổng hợp một chitosan từ tính mới (CCF), trong đó các hạt nano lõi-vỏ carbon-Fe3O4 đóng vai trò là phần từ tính. Cấu trúc, tính chất và hình thái của CCF từ tính được đặc trưng bởi các kỹ thuật FT-IR, XRD, EDAX, SEM và BET. Hiệu suất hấp phụ của nó đã được nghiên cứu để loại bỏ màu cam metyl khỏi các dung dịch nước bằng cách thay đổi các điều kiện thực nghiệm. Kết quả cho thấy sự hấp phụ nhanh chóng màu cam metyl trong phạm vi pH rộng từ 3-11 và dung lượng hấp phụ tối đa được tìm thấy là 425 mg g-1 ở 45 ° C. Kết quả động học hấp phụ chỉ ra rằng cơ chế hấp phụ được mô tả tốt hơn bởi phương trình bậc hai giả, trong khi khuếch tán lỗ rỗng là tốc độ kiểm soát động học hấp phụ. Hơn nữa, trong số các mô hình đẳng nhiệt khác nhau, các mô hình đẳng nhiệt Langmuir và Sips phù hợp tốt với dữ liệu thực nghiệm cân bằng ở các nhiệt độ khác nhau, cho thấy tính không đồng nhất bề mặt của chất hấp phụ. Chất hấp phụ thể hiện hiệu suất hấp phụ cao, so với một số chất hấp phụ chitosan khác được báo cáo trong tài liệu."} +{"idx": 1871126, "en": "Alpha-amylase producing strain KB 2216 was identified as Bacillus velezensis. The growth pattern showed that 72 h is the optimum incubation period of amylase production, which is a stationary period for the strain. By the purification process, maximum alpha-amylase activity was achieved up to 418.25 U/mL at 72 h of incubation, which was purified with 4.74 folds, 4230.32 U/mg specific activity, with 31.35% yield. The strain was found to produce an oligomeric alpha-amylase, namely Amy3. Amy3 was a trimeric macromolecule of 195 kDa with 62, 64, and 66 kDa subunits, as revealed by zymogram and SDS PAGE analyses. Amy3 was highly active at 55 °C and pH 5.5. It had shown the highest stability at pH 5.0-5.5 and between 0 ̊C and 4 ̊C. It did not require any metal cofactors, but it was inhibited by Ag2+, Hg2+ and Cd2+ divalent cations. Glucose and maltose were shown to be the end products of soluble starch digestion by Amy3. These interesting properties of Amy3 may be useful for many biotechnological applications in the future.", "gemini": "Chủng KB 2216 sản xuất alpha-amylase được xác định là Bacillus velezensis. Mô hình tăng trưởng cho thấy 72 giờ là thời gian ủ tối ưu của quá trình sản xuất amylase, đây là thời kỳ dừng của chủng. Theo quy trình tinh chế, hoạt độ alpha-amylase tối đa đạt tới 418,25 U / mL sau 72 giờ ủ, được tinh chế với 4,74 lần, hoạt độ riêng 4230,32 U / mg, với hiệu suất 31,35%. Chủng được phát hiện sản xuất một alpha-amylase dạng oligome, cụ thể là Amy3. Amy3 là một đại phân tử ba phân tử có khối lượng phân tử 195 kDa với các tiểu đơn vị 62, 64 và 66 kDa, như được tiết lộ bởi các phân tích zymogram và SDS PAGE. Amy3 có hoạt tính cao ở 55 ° C và pH 5,5. Nó đã biểu hiện độ ổn định cao nhất ở pH 5,0-5,5 và giữa 0 ̊C và 4 ̊C. Nó không yêu cầu bất kỳ cofactor kim loại nào, nhưng nó bị ức chế bởi các cation hai hóa trị Ag2 +, Hg2 + và Cd2 +. Glucose và maltose được chứng minh là sản phẩm cuối cùng của quá trình tiêu hóa tinh bột hòa tan bởi Amy3. Những tính chất thú vị này của Amy3 có thể hữu ích cho nhiều ứng dụng công nghệ sinh học trong tương lai."} +{"idx": 1871127, "en": "Nitrogen is one of the most important nutrients for wheat growth and has a critical influence on yield and quality. This study aims to examine how medium nitrogen level (240 kg/hm2) affects the starch granule development, starch accumulation, and structural characteristics of wheat starch. The results showed that nitrogen treatment could reduce the biosynthesis of starch and amylose, delay the degradation of starch in pericarp, and promote the proportion of B-type small starch granule in endosperm compared with those in the N0. In addition, the composition and distribution of starch granules were changed, the crystal structure in the inner lamellae and ordered structure in the external region of starch granules were affected, and the swelling power and solubility of starch during wheat development were increased. The effect of nitrogen treatment on the mRNA expression of enzymes related to starch biosynthesis or degradation varied in different developmental stages. During middle and later grain filling stages, AGPase, GBSSI, and GBSSII were lower, and SSS, SBE, and DBE were higher in N240 than in N0. This study indicated that nitrogen application at booting stage significantly affected the structural characteristics of starch, and ultimately determines its quality.", "gemini": "Nitơ là một trong những chất dinh dưỡng quan trọng nhất cho sự phát triển của lúa mì và có ảnh hưởng rất lớn đến năng suất và chất lượng. Nghiên cứu này nhằm mục đích kiểm tra mức nitơ trung bình (240 kg / ha) ảnh hưởng đến sự phát triển của hạt tinh bột, sự tích tụ tinh bột và các đặc điểm cấu trúc của tinh bột lúa mì như thế nào. Kết quả cho thấy xử lý nitơ có thể làm giảm quá trình sinh tổng hợp tinh bột và amylose, làm chậm quá trình phân hủy tinh bột trong vỏ quả và thúc đẩy tỷ lệ hạt tinh bột nhỏ loại B trong nội nhũ so với những hạt trong N0. Ngoài ra, thành phần và sự phân bố của hạt tinh bột đã thay đổi, cấu trúc tinh thể ở các phiến trong và cấu trúc có trật tự ở vùng ngoài của hạt tinh bột bị ảnh hưởng và lực trương nở và độ hòa tan của tinh bột trong quá trình phát triển của lúa mì tăng lên. Tác dụng của xử lý nitơ đối với biểu hiện mRNA của các enzym liên quan đến quá trình sinh tổng hợp hoặc phân hủy tinh bột khác nhau ở các giai đoạn phát triển khác nhau. Trong giai đoạn giữa và cuối của quá trình làm đầy hạt, AGPase, GBSSI và GBSSII thấp hơn, trong khi SSS, SBE và DBE cao hơn ở N240 so với N0. Nghiên cứu này chỉ ra rằng việc bón nitơ ở giai đoạn phân hóa đã ảnh hưởng đáng kể đến các đặc điểm cấu trúc của tinh bột và cuối cùng quyết định chất lượng của tinh bột."} +{"idx": 1871128, "en": "Different factors, such as basic fibroblast growth factor (bFGF) and kit ligand (KL), are used in ovarian cortical culture to promote activation of primordial follicles. In the present study, the effects of bFGF and KL, alone and in combination, were evaluated on human follicular activation and growth during in-situ cortical culture. Slow frozen-thawed human ovarian cortical tissues (n = 6) were cultured in 4 different groups: 1) control (base medium), 2) KL (base medium; BM + 100 ng/ml KL), 3) bFGF (BM + 100 ng/ml bFGF) and 4) bFGF + KL (BM + 100 ng/ml KL + 100 ng/ml bFGF) for a week. The proportion of morphologically normal and degenerated follicles at different developmental stages, secreted hormonal levels and specific gene expressions were compared. Although the proportion of growing follicles was higher than primordial counterpart in all cultured groups, no significant differences were observed among the cultured groups. In all cultured groups, anti-Müllerian hormone (AMH), progesterone and estradiol hormones levels increased after 7 days of culture; however, this increase was only significant for estradiol in the bFGF + KL group. The expression of Ki67 gene indicated an increase in ovarian cell proliferation in the three experimental groups compared to the control group, however this increment was only significant for the bFGF + KL group. It can be concluded that KL and bFGF factors individually have no beneficial effects on in-situ follicular growth, but their combination positively influences steroidogenesis of granulosa cells without significantly increasing the number of growing follicles.", "gemini": "Các yếu tố khác nhau, chẳng hạn như yếu tố tăng trưởng nguyên bào sợi cơ bản (bFGF) và phối tử kit (KL), được sử dụng trong nuôi cấy vỏ buồng trứng để thúc đẩy hoạt hóa nang nguyên thủy. Trong nghiên cứu hiện tại, tác dụng của bFGF và KL, riêng lẻ và kết hợp, đã được đánh giá trên hoạt hóa và tăng trưởng nang ở người trong quá trình nuôi cấy vỏ tại chỗ. Các mô vỏ buồng trứng của người đông lạnh-rã đông chậm (n = 6) được nuôi cấy trong 4 nhóm khác nhau: 1) đối chứng (môi trường cơ bản), 2) KL (môi trường cơ bản; BM + 100 ng / ml KL), 3) bFGF (BM + 100 ng / ml bFGF) và 4) bFGF + KL (BM + 100 ng / ml KL + 100 ng / ml bFGF) trong một tuần. Tỷ lệ nang bình thường về mặt hình thái và thoái hóa ở các giai đoạn phát triển khác nhau, mức độ nội tiết tố tiết ra và biểu hiện gen cụ thể đã được so sánh. Mặc dù tỷ lệ nang đang phát triển cao hơn so với nang nguyên thủy trong tất cả các nhóm được nuôi cấy, nhưng không có sự khác biệt đáng kể nào được quan sát thấy giữa các nhóm được nuôi cấy. Trong tất cả các nhóm được nuôi cấy, nồng độ hormone chống Müller (AMH), progesterone và estradiol tăng sau 7 ngày nuôi cấy; tuy nhiên, sự gia tăng này chỉ có ý nghĩa đối với estradiol trong nhóm bFGF + KL. Biểu hiện của gen Ki67 cho thấy sự gia tăng trong sự tăng sinh tế bào buồng trứng ở ba nhóm thực nghiệm so với nhóm đối chứng, tuy nhiên sự gia tăng này chỉ có ý nghĩa đối với nhóm bFGF + KL. Có thể kết luận rằng các yếu tố KL và bFGF riêng lẻ không có tác dụng có lợi đối với sự phát triển nang tại chỗ, nhưng sự kết hợp của chúng ảnh hưởng tích cực đến quá trình sản xuất steroid của các tế bào hạt mà không làm tăng đáng kể số lượng nang đang phát triển."} +{"idx": 1871129, "en": "The Wnt signalling pathway plays an important role in development, disease, and normal tissue function. Mathematical models for Wnt signalling have predominantly focused on quantitatively predicting changes in steady-state β-catenin concentrations (the main downstream protein regulated by canonical Wnt signalling). One of the genes targeted for expression by Wnt/β-catenin signalling is the negative Wnt regulator Axin2. Recently, a number of authors have indicated a potential theoretical role of Axin2 feedback to induce oscillatory behaviour in the pathway and this has been observed in a number of detailed mathematical models. Due to the complexity of these models, the investigations to date have been limited to numerical experiments and parameter sensitivity analyses. In this manuscript, we study the fundamental structure of the dynamical system underlying the Wnt signalling mechanism with Axin2 feedback to gain some insight into why and when oscillations occur in models with this structure. We semi-rigorously analyse three simple models and, for these models, gain deep understanding of the characteristic set of conditions that are necessary and sufficient for oscillations to be induced. We discuss the possible biological consequences of these findings for Wnt signalling pathway oscillations. They include; to promote oscillations (1) Keeping all other parameters constant, the Wnt signal strength should neither be too high or too low but within a single finite window of values, (2) Wnt receptor complexes should fully deactivate Axin rather than temporarily deconstruct it from other scaffold proteins, (3) In the absence of stochastic effects or more complicated mechanisms, a critical delay in Axin2 feedback in the system is necessary, (4) Deactivation of Axin by the Wnt receptor complex needs to be critically efficient relative to β-catenin removal by Axin, and (5) conditions necessary are less strict if Axin2 feedback occurs after a fixed time rather than a Poisson-distributed time with the same average.", "gemini": "Con đường truyền tín hiệu Wnt đóng vai trò quan trọng trong sự phát triển, bệnh tật và chức năng mô bình thường. Các mô hình toán học cho tín hiệu Wnt chủ yếu tập trung vào việc dự đoán định lượng những thay đổi về nồng độ β-catenin trạng thái ổn định (chủ yếu là protein hạ nguồn được điều chỉnh bởi tín hiệu Wnt điển hình). Một trong những gen được nhắm mục tiêu để biểu hiện bởi tín hiệu Wnt / β-catenin là chất điều hòa Wnt âm tính Axin2. Gần đây, một số tác giả đã chỉ ra một vai trò lý thuyết tiềm ẩn của phản hồi Axin2 để gây ra hành vi dao động trong con đường này và điều này đã được quan sát thấy trong một số mô hình toán học chi tiết. Do tính phức tạp của các mô hình này, các cuộc điều tra cho đến nay đã bị giới hạn ở các thí nghiệm số và phân tích độ nhạy của tham số. Trong bản thảo này, chúng tôi nghiên cứu cấu trúc cơ bản của hệ thống động học cơ bản của cơ chế truyền tín hiệu Wnt với phản hồi Axin2 để hiểu sâu hơn về lý do và thời điểm dao động xảy ra trong các mô hình có cấu trúc này. Chúng tôi phân tích ba mô hình đơn giản một cách bán nghiêm ngặt và đối với các mô hình này, chúng tôi hiểu sâu sắc về tập hợp các điều kiện đặc trưng cần thiết và đủ để gây ra dao động. Chúng tôi thảo luận về các hậu quả sinh học có thể có của những phát hiện này đối với dao động của con đường truyền tín hiệu Wnt. Chúng bao gồm; để thúc đẩy dao động (1) Giữ tất cả các tham số khác không đổi, cường độ tín hiệu Wnt không được quá cao hoặc quá thấp mà phải nằm trong một cửa sổ giá trị hữu hạn duy nhất, (2) Các phức hợp thụ thể Wnt phải vô hiệu hóa hoàn toàn Axin thay vì tạm thời phá hủy nó khỏi các protein khung khác, (3) Trong trường hợp không có hiệu ứng ngẫu nhiên hoặc các cơ chế phức tạp hơn, một sự chậm trễ quan trọng trong phản hồi Axin2 trong hệ thống là cần thiết, (4) Việc vô hiệu hóa Axin bởi phức hợp thụ thể Wnt cần phải hiệu quả quan trọng so với việc loại bỏ β-catenin bởi Axin, và (5) các điều kiện cần thiết sẽ ít nghiêm ngặt hơn nếu phản hồi Axin2 xảy ra sau một thời gian cố định chứ không phải là thời gian phân phối Poisson với cùng một giá trị trung bình."} +{"idx": 1871130, "en": "BACKGROUND Noninvasive monitoring of fetal development and the early detection of pregnancy-associated complications is challenging, largely because of the lack of information about the molecular spectrum during pregnancy. Recently, cell-free DNA in plasma was found to reflect the global nucleosome footprint and status of gene expression and showed potential for noninvasive health monitoring during pregnancy. OBJECTIVE We aimed to test the relationships between plasma cell-free DNA profiles and pregnancy biology and evaluate the use of a cell-free DNA profile as a noninvasive method for physiological and pathologic status monitoring during pregnancy. STUDY DESIGN We used genome cell-free DNA sequencing data generated from noninvasive prenatal testing in a total of 2937 pregnant women. For each physiological and pathologic condition, features of the cell-free DNA profile were identified using the discovery cohort, and support vector machine classifiers were built and evaluated using independent training and validation cohorts. RESULTS We established nucleosome occupancy profiles at transcription start sites in different gestational trimesters, demonstrated the relationships between gene expression and cell-free DNA coverage at transcription start sites, and showed that the cell-free DNA profiles at transcription start sites represented the biological processes of pregnancy. In addition, using cell-free DNA data, nucleosome profiles of transcription factor binding sites were identified to reflect the transcription factor footprint, which may help to reveal the molecular mechanisms underlying pregnancy. Finally, by using machine-learning models on low-coverage noninvasive prenatal testing data, we evaluated the use of cell-free DNA nucleosome profiles for distinguishing gestational trimesters, fetal sex, and fetal trisomy 21 and highlighted its potential utility for predicting physiological and pathologic fetal conditions by using low-coverage noninvasive prenatal testing data. CONCLUSION Our analyses profiled nucleosome footprints and regulatory networks during pregnancy and established a noninvasive proof-of-principle methodology for health monitoring during pregnancy.", "gemini": "BỐI CẢNH Theo dõi không xâm lấn sự phát triển của thai nhi và phát hiện sớm các biến chứng liên quan đến thai kỳ là một thách thức, phần lớn là do thiếu thông tin về phổ phân tử trong thời kỳ mang thai. Gần đây, người ta phát hiện ra rằng DNA không có tế bào trong huyết tương phản ánh dấu vết nucleosome toàn cầu và trạng thái biểu hiện gen và cho thấy tiềm năng theo dõi sức khỏe không xâm lấn trong thời kỳ mang thai. MỤC TIÊU Chúng tôi nhằm mục đích kiểm tra mối quan hệ giữa hồ sơ DNA không có tế bào trong huyết tương và sinh học thai kỳ và đánh giá việc sử dụng hồ sơ DNA không có tế bào như một phương pháp không xâm lấn để theo dõi tình trạng sinh lý và bệnh lý trong thời kỳ mang thai. THIẾT KẾ NGHIÊN CỨU Chúng tôi đã sử dụng dữ liệu giải trình tự DNA không có tế bào trong bộ gen được tạo ra từ xét nghiệm trước sinh không xâm lấn ở tổng số 2937 phụ nữ mang thai. Đối với từng tình trạng sinh lý và bệnh lý, các đặc điểm của cấu hình DNA không có tế bào được xác định bằng cách sử dụng nhóm khám phá và các phân loại máy vectơ hỗ trợ được xây dựng và đánh giá bằng cách sử dụng các nhóm đào tạo và xác nhận độc lập. KẾT QUẢ Chúng tôi đã thiết lập các cấu hình chiếm dụng nucleosome tại các vị trí bắt đầu phiên mã trong các tam cá nguyệt khác nhau của thai kỳ, chứng minh mối quan hệ giữa biểu hiện gen và phạm vi phủ sóng DNA không có tế bào tại các vị trí bắt đầu phiên mã và cho thấy các cấu hình DNA không có tế bào tại các vị trí bắt đầu phiên mã đại diện cho các quá trình sinh học của thai kỳ. Ngoài ra, bằng cách sử dụng dữ liệu DNA không có tế bào, các cấu hình nucleosome của các vị trí liên kết yếu tố phiên mã đã được xác định để phản ánh dấu vết yếu tố phiên mã, điều này có thể giúp làm sáng tỏ các cơ chế phân tử cơ bản của thai kỳ. Cuối cùng, bằng cách sử dụng các mô hình học máy trên dữ liệu xét nghiệm trước sinh không xâm lấn có độ phủ sóng thấp, chúng tôi đã đánh giá việc sử dụng các cấu hình nucleosome DNA không có tế bào để phân biệt các tam cá nguyệt của thai kỳ, giới tính thai nhi và trisomy 21 của thai nhi và nhấn mạnh khả năng hữu ích của nó để dự đoán tình trạng sinh lý và bệnh lý của thai nhi bằng cách sử dụng dữ liệu xét nghiệm trước sinh không xâm lấn có độ phủ sóng thấp. KẾT LUẬN Các phân tích của chúng tôi đã lập hồ sơ dấu vết nucleosome và mạng lưới điều hòa trong thời kỳ mang thai và thiết lập một phương pháp luận không xâm lấn để theo dõi sức khỏe trong thời kỳ mang thai."} +{"idx": 1871131, "en": "Human data collected using noninvasive imaging techniques have established the importance of parietal regions towards episodic memory retrieval, including the angular gyrus and posterior cingulate cortex. Such regions comprise part of a putative core episodic retrieval network. In free recall, comparisons between contextually appropriate and inappropriate recall events (i.e. prior list intrusions) provide the opportunity to study memory retrieval networks supporting veridical recall, and existing findings predict that differences in electrical activity in these brain regions should be identified according to the accuracy of recall. However, prior iEEG studies, utilizing principally subdural grid electrodes, have not fully characterized brain activity in parietal regions during memory retrieval and have not examined connectivity between core recollection areas and the hippocampus or prefrontal cortex. Here, we employed a data set obtained from 100 human patients implanted with stereo EEG electrodes for seizure mapping purposes as they performed a free recall task. This data set allowed us to separately analyze activity in midline versus lateral parietal brain regions, and in anterior versus posterior hippocampus, to identify areas in which retrieval-related activity predicted the recollection of a correct versus an incorrect memory. With the wide coverage afforded by the stereo EEG approach, we were also able to examine interregional connectivity. Our key findings were that differences in gamma band activity in the angular gyrus, precuneus, posterior temporal cortex, and posterior (more than anterior) hippocampus discriminated accurate versus inaccurate recall as well as active retrieval versus memory search. The left angular gyrus exhibited a significant power decrease preceding list intrusions as well as unique phase-amplitude coupling properties, whereas the prefrontal cortex was unique in exhibiting a power increase during list intrusions. Analysis of connectivity revealed significant hemispheric asymmetry, with relatively sparse left-sided functional connections compared to the right hemisphere. One exception to this finding was elevated connectivity between the prefrontal cortex and left angular gyrus. This finding is interpreted as evidence for the engagement of prefrontal cortex in memory monitoring and mnemonic decision-making.", "gemini": "Dữ liệu ở người thu thập được bằng các kỹ thuật hình ảnh không xâm lấn đã thiết lập tầm quan trọng của các vùng đỉnh đối với việc truy xuất trí nhớ theo từng giai đoạn, bao gồm hồi góc và vỏ não vành sau. Các vùng như vậy bao gồm một phần của mạng lưới truy xuất theo từng giai đoạn cốt lõi được cho là. Trong việc tự do nhớ lại, các so sánh giữa các sự kiện nhớ lại phù hợp và không phù hợp với ngữ cảnh (tức là sự xâm nhập của danh sách trước đó) cung cấp cơ hội để nghiên cứu các mạng lưới truy xuất trí nhớ hỗ trợ việc nhớ lại đúng sự thật và các phát hiện hiện tại dự đoán rằng sự khác biệt về hoạt động điện trong các vùng não này nên được xác định theo độ chính xác của việc nhớ lại. Tuy nhiên, các nghiên cứu iEEG trước đây, chủ yếu sử dụng điện cực lưới dưới màng cứng, vẫn chưa mô tả đầy đủ hoạt động của não ở các vùng đỉnh trong quá trình truy xuất trí nhớ và chưa kiểm tra kết nối giữa các vùng hồi tưởng cốt lõi với hồi hải mã hoặc vỏ não trước trán. Ở đây, chúng tôi sử dụng một tập dữ liệu thu được từ 100 bệnh nhân là người được cấy điện cực EEG nổi để lập bản đồ cơn động kinh khi họ thực hiện nhiệm vụ tự do nhớ lại. Tập dữ liệu này cho phép chúng tôi phân tích riêng hoạt động ở các vùng não đỉnh giữa so với bên và ở hồi hải mã trước so với sau, để xác định các khu vực mà hoạt động liên quan đến truy xuất dự đoán việc nhớ lại một ký ức chính xác so với một ký ức không chính xác. Với phạm vi phủ sóng rộng do phương pháp EEG nổi mang lại, chúng tôi cũng có thể kiểm tra kết nối giữa các vùng. Những phát hiện chính của chúng tôi là sự khác biệt về hoạt động băng tần gamma ở hồi góc, tiền đòn, vỏ não thái dương sau và hồi hải mã sau (hơn là trước) phân biệt việc nhớ lại chính xác so với không chính xác cũng như việc truy xuất chủ động so với tìm kiếm trí nhớ. Hồi góc trái biểu hiện sự giảm công suất đáng kể trước khi xâm nhập vào danh sách cũng như các đặc tính liên kết pha-biên độ độc đáo, trong khi vỏ não trước trán có đặc điểm riêng là biểu hiện sự gia tăng công suất trong quá trình xâm nhập danh sách. Phân tích kết nối cho thấy sự không đối xứng bán cầu đáng kể, với các kết nối chức năng bên trái tương đối thưa thớt so với bán cầu phải. Một ngoại lệ đối với phát hiện này là kết nối nâng cao giữa vỏ não trước trán và hồi góc trái. Phát hiện này được hiểu là bằng chứng cho thấy sự tham gia của vỏ não trước trán vào việc theo dõi trí nhớ và ra quyết định ghi nhớ."} +{"idx": 1871132, "en": "Lymphogranuloma venereum (LGV) is an emerging disease in men who have sex with men (MSM): the incidence was 1.15 cases per 100,000 population in Spain in 2017. Patients with LGV characteristically have severe proctitis that can cause abscesses, fistulas, and anal stenosis. Genital ulcers and inflammatory inguinal adenopathy may occasionally be present. The aim of this study was to describe a series of patients with LGV treated in a public health service hospital in Andalusia, Spain. MATERIAL AND METHODS Retrospective, observational description of a series of patients diagnosed with LGV. We gathered epidemiologic, clinical, microbiologic, and treatment data. Patients' sexual behaviors were also noted. RESULTS We found 17 cases of LGV diagnosed in MSM between October 2016 and May 2019. Twelve of the patients were also infected with the human immunodeficiency virus, and 13 had severe proctitis with ulcers in the anal canal and rectum. Four patients had genital or inguinal manifestations. The following high-risk sexual behaviors were on record: a high number of sexual partners, receptive anal sex with strangers and without a condom, seeking sexual partners online, participation in group sex, and sex with partners from outside Andalusia. Chlamydia trachomatis serovar L2 was identified in all cases, and the infection responded well to oral doxycycline. Two patients with the most characteristic form of LGV required longer treatment cycles. Three required surgery. CONCLUSIONS When symptomatic proctitis is found in MSM who engage in high-risk sex, the LGV exudate should be sampled and the C trachomatis serovar identified. Genital ulcers or inguinal buboes are also highly suggestive of LGV. Clinical suspicion and early treatment are the keys to preventing complications and disease transmission.", "gemini": "Bệnh hạch bạch huyết sinh dục (LGV) là một căn bệnh mới nổi ở những người đàn ông quan hệ tình dục với đàn ông (MSM): tỷ lệ mắc bệnh là 1,15 trường hợp trên 100.000 dân ở Tây Ban Nha vào năm 2017. Những bệnh nhân mắc bệnh LGV thường có viêm trực tràng nặng có thể gây ra áp xe, lỗ rò và hẹp hậu môn. Thỉnh thoảng có thể có loét bộ phận sinh dục và hạch bẹn viêm. Mục đích của nghiên cứu này là mô tả một loạt các bệnh nhân mắc bệnh LGV được điều trị tại một bệnh viện dịch vụ y tế công ở Andalusia, Tây Ban Nha. PHƯƠNG PHÁP VÀ VẬT LIỆU Mô tả quan sát, hồi cứu một loạt các bệnh nhân được chẩn đoán mắc bệnh LGV. Chúng tôi đã thu thập dữ liệu về dịch tễ học, lâm sàng, vi sinh và điều trị. Hành vi tình dục của bệnh nhân cũng được ghi nhận. KẾT QUẢ Chúng tôi đã tìm thấy 17 trường hợp mắc bệnh LGV được chẩn đoán ở MSM trong khoảng thời gian từ tháng 10 năm 2016 đến tháng 5 năm 2019. Mười hai bệnh nhân cũng bị nhiễm vi-rút gây suy giảm miễn dịch ở người và 13 bệnh nhân bị viêm trực tràng nặng với loét ở ống hậu môn và trực tràng. Bốn bệnh nhân có biểu hiện ở bộ phận sinh dục hoặc bẹn. Các hành vi tình dục có nguy cơ cao sau đây đã được ghi nhận: số bạn tình nhiều, quan hệ tình dục qua đường hậu môn với người lạ và không sử dụng bao cao su, tìm bạn tình trực tuyến, tham gia quan hệ tình dục tập thể và quan hệ tình dục với bạn tình từ bên ngoài Andalusia. Chlamydia trachomatis huyết thanh L2 được xác định trong tất cả các trường hợp và nhiễm trùng đáp ứng tốt với doxycycline uống. Hai bệnh nhân có dạng LGV đặc trưng nhất cần phải điều trị trong các chu kỳ dài hơn. Ba bệnh nhân cần phẫu thuật. KẾT LUẬN Khi phát hiện viêm trực tràng có triệu chứng ở MSM có quan hệ tình dục có nguy cơ cao, nên lấy mẫu dịch tiết LGV và xác định huyết thanh C trachomatis. Loét bộ phận sinh dục hoặc hạch bẹn cũng rất gợi ý đến bệnh LGV. Nghi ngờ lâm sàng và điều trị sớm là chìa khóa để ngăn ngừa các biến chứng và lây truyền bệnh."} +{"idx": 1871133, "en": "OBJECTIVES Currently, the global interests tend to take advantage of the plant world as a renewable source of a natural and effective molecule, to find an eco-friendly, cost-effective, and less toxic alternative to the current synthetic pesticide. In this context, the present research was carried out in an attempt to study the insecticidal activity of extracts and pigments derived from the green plant Spinacia oleracea and the green alga Ulva lactuca against the fruit fly Drosophila melanogaster as an alternative to chemical insecticide. METHODS The toxicity of the aqueous, acetonic and ethanolic extracts as well as of the purified pigments (Chlorophylls and carotenoids) was determined by complementary in vivo tests (application by spraying oranges, toxicity by ingestion and repellent activity). Interestingly, each one of these methods corresponds to a specific mode of exposure. RESULTS Results showed that acetone extracts, which are rich in green pigments, present the best insecticidal activities. On the other hand, the purified chlorophyllian pigments exhibited an interesting activity only by spraying method. Regarding the repellent activity, the aqueous extract of spinach displayed higher effectiveness. CONCLUSION Our study suggests the potential of tested plant and algal extracts, as well as of chlorophyllian pigments, to provide a safer alternative way to the use of synthetic pesticides.", "gemini": "MỤC TIÊU Hiện nay, các lợi ích toàn cầu có xu hướng tận dụng thế giới thực vật như một nguồn phân tử tự nhiên và hiệu quả có thể tái tạo, để tìm ra một giải pháp thay thế thân thiện với môi trường, hiệu quả về chi phí và ít độc hại hơn so với thuốc trừ sâu tổng hợp hiện tại. Trong bối cảnh này, nghiên cứu hiện tại được thực hiện nhằm mục đích nghiên cứu hoạt tính diệt côn trùng của các chiết xuất và sắc tố có nguồn gốc từ thực vật xanh Spinacia oleracea và tảo xanh Ulva lactuca chống lại ruồi giấm Drosophila melanogaster như một giải pháp thay thế cho thuốc trừ sâu hóa học. PHƯƠNG PHÁP Độc tính của các chiết xuất nước, axeton và etanol cũng như các sắc tố tinh khiết (diệp lục và carotenoid) được xác định bằng các xét nghiệm in vivo bổ sung (phun lên cam, độc tính khi ăn và hoạt tính chống côn trùng). Điều thú vị là mỗi phương pháp này tương ứng với một chế độ phơi nhiễm cụ thể. KẾT QUẢ Kết quả cho thấy các chiết xuất axeton, giàu sắc tố xanh, có hoạt tính diệt côn trùng tốt nhất. Mặt khác, các sắc tố diệp lục tinh khiết chỉ biểu hiện hoạt tính thú vị thông qua phương pháp phun. Về hoạt tính chống côn trùng, chiết xuất nước của rau bina cho thấy hiệu quả cao hơn. KẾT LUẬN Nghiên cứu của chúng tôi cho thấy tiềm năng của các chiết xuất thực vật và tảo đã được thử nghiệm, cũng như các sắc tố diệp lục, để cung cấp một cách thay thế an toàn hơn cho việc sử dụng thuốc trừ sâu tổng hợp."} +{"idx": 1871134, "en": "Histidine decarboxylase (HDC; EC 4.1.1.22), an enzyme that catalyzes histamine synthesis with high substrate specificity, is a member of the group II pyridoxal 5'-phosphate (PLP) -dependent decarboxylase family. Tyrosine is a conserved residue among group II PLP-dependent decarboxylases. Human HDC has a Y334 located on a catalytically important loop at the active site. In this study, we demonstrated that a HDC Y334F mutant is capable of catalyzing the decarboxylation-dependent oxidative deamination of histidine to yield imidazole acetaldehyde. Replacement of the active-site Tyr with Phe in group II PLP-dependent decarboxylases, including mammalian aromatic amino acid decarboxylase, plant tyrosine/DOPA decarboxylase, and plant tryptophan decarboxylase, is expected to result in the same functional change, given that a Y-to-F substitution at the corresponding residue (number 260) in the HDC of Morganella morganii, another group II PLP-dependent decarboxylase, yielded the same effect. Thus, it was suggested that the loss of the OH moiety from the active-site Tyr residue of decarboxylase uniquely converts the enzyme to an aldehyde synthase.", "gemini": "Histidine decarboxylase (HDC; EC 4.1.1.22), một enzyme xúc tác tổng hợp histamine với tính đặc hiệu cơ chất cao, là thành viên của nhóm II pyridoxal 5'-phosphate (PLP) - họ decarboxylase phụ thuộc. Tyrosine là một chất bảo tồn giữa các decarboxylase phụ thuộc PLP nhóm II. HDC của người có Y334 nằm trên một vòng lặp quan trọng về mặt xúc tác tại vị trí hoạt động. Trong nghiên cứu này, chúng tôi đã chứng minh rằng một đột biến HDC Y334F có khả năng xúc tác quá trình deamin oxy hóa phụ thuộc vào decarboxyl hóa của histidine để tạo ra imidazole acetaldehyde. Việc thay thế Tyr tại vị trí hoạt động bằng Phe trong các decarboxylase phụ thuộc PLP nhóm II, bao gồm decarboxylase axit amin thơm ở động vật có vú, tyrosine/DOPA decarboxylase thực vật và tryptophan decarboxylase thực vật, dự kiến sẽ dẫn đến cùng một thay đổi chức năng, vì sự thay thế Y thành F ở vị trí tương ứng (số 260) trong HDC của Morganella morganii, một decarboxylase phụ thuộc PLP nhóm II khác, cho kết quả tương tự. Do đó, người ta đã đưa ra giả thuyết rằng sự mất nhóm OH khỏi chất Tyr tại vị trí hoạt động của decarboxylase chuyển đổi enzyme thành một chất tổng hợp aldehyde."} +{"idx": 1871135, "en": "Applications of non-thermal plasma (NTP) discharges in medicine, particularly cancer therapy, have increased in recent years. The aim of the present study was to investigate the advantages of the combined application of NTP-irradiated acetated Ringer's solution (PAA) and hyperthermia, a heat treatment at 42 °C, on A549 cancer cell death and elucidate the underlying mechanisms. Cell death was enhanced more by the above combined treatment and was accompanied by increases in intracellular calcium ([Ca2+]i). The activation of transient receptor potential melastatin 2 (TRPM2) may enhance cell death because the addition of TRPM2 inhibitors and knockdown of TRPM2 significantly abrogated the above phenomena. TRPM2 is a temperature-sensitive, Ca2+-permeable, non-elective cation channel and hydrogen peroxide (H2O2) and ADP ribose are its main agonists. PAA functioned as a donor of reactive oxygen species, mainly H2O2, and a treatment with PAA under hyperthermia induced both mitochondrial and nuclear damage with DNA breaks. The activation of poly(ADP-ribose) polymerase-1 as the DNA repair mechanism induced TRPM2 activation because this enzyme accumulates ADP ribose. The sensitivity of fibroblasts as normal cells to PAA was less than that of A549 cells. These results suggest that hyperthermia synergistically induces the sensitivity of cancer cells to PAA.", "gemini": "Các ứng dụng của các chất thải plasma không nhiệt (NTP) trong y học, đặc biệt là liệu pháp điều trị ung thư, đã tăng lên trong những năm gần đây. Mục đích của nghiên cứu hiện tại là để điều tra những lợi thế của việc kết hợp ứng dụng dung dịch Ringer axetat được chiếu xạ NTP (PAA) và tăng thân nhiệt, một phương pháp điều trị bằng nhiệt ở 42 °C, đối với tế bào ung thư A549 và làm sáng tỏ các cơ chế cơ bản. Cái chết của tế bào được tăng cường nhiều hơn bởi phương pháp điều trị kết hợp trên và đi kèm với sự gia tăng canxi nội bào ([Ca2+]i). Sự hoạt hóa của thụ thể tiềm thời melastatin 2 (TRPM2) có thể làm tăng chết tế bào vì việc bổ sung các chất ức chế TRPM2 và hạ gục TRPM2 đã làm giảm đáng kể các hiện tượng trên. TRPM2 là một kênh cation không chọn lọc, thấm Ca2+, nhạy cảm với nhiệt độ và hydro peroxide (H2O2) và ADP ribose là các chất chủ vận chính của nó. PAA hoạt động như một chất tặng các loài oxy phản ứng, chủ yếu là H2O2, và phương pháp điều trị bằng PAA dưới nhiệt độ cao gây tổn thương cả ty thể và nhân với các đứt gãy DNA. Sự hoạt hóa của poly(ADP-ribose) polymerase-1 như là cơ chế sửa chữa DNA đã gây ra hoạt hóa TRPM2 vì enzyme này tích tụ ADP ribose. Độ nhạy của nguyên bào sợi như các tế bào bình thường đối với PAA thấp hơn so với tế bào A549. Những kết quả này cho thấy rằng tăng thân nhiệt hiệp đồng gây ra độ nhạy của các tế bào ung thư đối với PAA."} +{"idx": 1871136, "en": "Traumatic brain injury affects millions of people each year and is an established risk factor for addiction. Recent animal studies have causally demonstrated that injuries can increase drug self-administration across a variety of substances. One potential behavioral mediator for this finding is an increased responsivity to drug-associated cues. This endophenotype can be identified by profiling non-drug-related behaviors. The current study evaluated several paradigms (conditioned approach, conditioned reinforcement, extinction from variable interval responding, conditioned facilitation) to determine how rats with a frontal TBI differed in their response to Pavlovian conditioning in response to food-paired cues. Surprisingly, rats with a TBI demonstrated increased goal-tracking in a conditioned approach paradigm and exerted less effort for a conditioned reinforcer. Moreover, they had slightly facilitated extinction (as demonstrated by significantly larger interresponse times) in the face of reinforcer-associated cues. Despite these effects, TBI rats still demonstrated conditioned facilitation to an auditory stimulus. Together, these effects suggest a phenotype in the opposite direction of what might be anticipated. Cues still served a strong discriminative function and altered behavior; however, they did not function as strong conditioned reinforcers for TBI animals. One potential reason for this is that substantial changes to the dopamine system after TBI may reduce the conditioned reinforcing effects of cues, but sensitize the brain to potent drugs of abuse. More research will be needed to determine whether this is the case.", "gemini": "Chấn thương sọ não ảnh hưởng đến hàng triệu người mỗi năm và là một yếu tố nguy cơ đã được xác định đối với chứng nghiện. Các nghiên cứu trên động vật gần đây đã chứng minh một cách có nguyên nhân rằng chấn thương có thể làm tăng tự dùng thuốc đối với nhiều loại chất khác nhau. Một chất trung gian hành vi tiềm ẩn cho phát hiện này là khả năng phản ứng tăng lên với các tín hiệu liên quan đến thuốc. Nội hình này có thể được xác định bằng cách lập hồ sơ các hành vi không liên quan đến thuốc. Nghiên cứu hiện tại đã đánh giá một số mô hình (phương pháp tiếp cận có điều kiện, củng cố có điều kiện, sự tuyệt chủng từ phản ứng khoảng thời gian biến đổi, tạo điều kiện thuận lợi) để xác định cách mà những con chuột bị TBI ở vùng trán khác nhau về phản ứng của chúng đối với điều kiện hóa Pavlovian để đáp ứng với các tín hiệu ghép nối thức ăn. Đáng ngạc nhiên, những con chuột bị TBI đã chứng minh khả năng theo dõi mục tiêu tăng lên trong một mô hình tiếp cận có điều kiện và ít nỗ lực hơn cho một chất củng cố có điều kiện. Hơn nữa, chúng đã tạo điều kiện thuận lợi cho sự tuyệt chủng (như được chứng minh bằng thời gian phản ứng giữa các lần lớn hơn đáng kể) khi đối mặt với các tín hiệu liên quan đến chất củng cố. Mặc dù có những tác động này, những con chuột bị TBI vẫn chứng minh được sự tạo điều kiện thuận lợi cho một kích thích thính giác. Cùng nhau, những tác động này cho thấy một kiểu hình theo hướng ngược lại với những gì có thể dự đoán. Các tín hiệu vẫn đóng vai trò phân biệt mạnh mẽ và thay đổi hành vi; tuy nhiên, chúng không hoạt động như chất củng cố có điều kiện mạnh mẽ đối với động vật bị TBI. Một lý do tiềm ẩn cho điều này là những thay đổi đáng kể đối với hệ thống dopamine sau TBI có thể làm giảm tác dụng củng cố có điều kiện của các tín hiệu, nhưng làm cho não nhạy cảm với các loại thuốc gây nghiện mạnh. Cần nhiều nghiên cứu hơn để xác định xem đây có phải là trường hợp hay không."} +{"idx": 1871137, "en": "OBJECTIVE To evaluate the efficacy of repeat endoscopic electrofulguration in women with antibiotic-refractory, recurrent urinary tract infections (RUTIs) with persistent symptoms after 1 electrofulguration. METHODS An institutional review board-approved, prospectively maintained database of non-neurogenic women with RUTIs, persistent symptoms and endoscopic findings of bladder wall inflammation after 1 electrofulguration, and minimum 6 months follow-up was reviewed. Endoscopic success was defined as complete resolution of previous lesions without new lesions seen during office cystoscopy 6 months after second electrofulguration. Clinical success was defined as no urinary tract infections at last follow-up; improvement as 1-2 treated infections/year; and failure as ≥3 treated infections/year, daily antibiotic suppression, or another electrofulguration (third or fourth). RESULTS From 2006 to 2018, 58/70 (83%) women with median age 70 years and median follow-up 26 months were included, and 53/58 had endoscopic data 6 months postsecond electrofulguration. Endoscopic success was noted in 26 (49%), and 21/26 had <3 UTIs within the last year of follow-up, vs 2/27 (7%) with endoscopic failure (P = .001). Among those with clinical failure, 6/30 (20%) remained on suppressive antibiotics, 9/30 (30%) required intravenous antibiotic courses, and 2 proceeded to cystectomy. Of 24 women who underwent a third electrofulguration, 11/24 (46%) were clinically successful or improved at median 22 months follow-up. Urine cultures from the year of last follow-up revealed extended-spectrum beta lactamase producing strains (50%) and strains resistant to >3 antibiotics (43%). CONCLUSION Women with persistent RUTIs following 1 electrofulguration may benefit from a second or even third procedure.", "gemini": "MỤC TIÊU Đánh giá hiệu quả của việc đốt điện nội soi lặp lại ở những phụ nữ bị nhiễm trùng đường tiết niệu tái phát (RUTI) kháng kháng sinh với các triệu chứng dai dẳng sau 1 lần đốt điện. PHƯƠNG PHÁP Một cơ sở dữ liệu được duy trì theo viễn cảnh, được hội đồng đánh giá của tổ chức chấp thuận về những phụ nữ không bị thần kinh với RUTI, các triệu chứng dai dẳng và các phát hiện nội soi về tình trạng viêm thành bàng quang sau 1 lần đốt điện và theo dõi tối thiểu 6 tháng đã được xem xét. Hiệu quả nội soi được định nghĩa là tình trạng tổn thương trước đó được giải quyết hoàn toàn mà không thấy tổn thương mới trong quá trình soi bàng quang tại phòng khám 6 tháng sau lần đốt điện thứ hai. Hiệu quả lâm sàng được định nghĩa là không bị nhiễm trùng đường tiết niệu ở lần theo dõi cuối cùng; cải thiện là 1-2 lần nhiễm trùng được điều trị/năm; và thất bại là ≥3 lần nhiễm trùng được điều trị/năm, phải dùng kháng sinh ức chế hàng ngày hoặc phải đốt điện một lần nữa (lần thứ ba hoặc thứ tư). KẾT QUẢ Từ năm 2006 đến năm 2018, 58/70 (83%) phụ nữ có độ tuổi trung bình là 70 và thời gian theo dõi trung bình là 26 tháng đã được đưa vào nghiên cứu, và 53/58 người có dữ liệu nội soi 6 tháng sau lần đốt điện thứ hai. Hiệu quả nội soi được ghi nhận ở 26 (49%) người, và 21/26 người có <3 lần nhiễm trùng đường tiết niệu trong năm cuối cùng của thời gian theo dõi, so với 2/27 (7%) người có thất bại nội soi (P = .001). Trong số những người có thất bại lâm sàng, 6/30 (20%) vẫn dùng kháng sinh ức chế, 9/30 (30%) cần dùng kháng sinh đường tĩnh mạch và 2 người đã cắt bỏ bàng quang. Trong số 24 phụ nữ đã trải qua lần đốt điện thứ ba, 11/24 (46%) đã đạt được hiệu quả lâm sàng hoặc cải thiện ở thời điểm theo dõi trung bình 22 tháng. Các mẫu nuôi cấy nước tiểu từ năm của lần theo dõi cuối cùng cho thấy các chủng vi khuẩn sản xuất beta lactamase phổ rộng (50%) và các chủng kháng >3 loại kháng sinh (43%). KẾT LUẬN Những phụ nữ bị RUTI dai dẳng sau 1 lần đốt điện có thể được hưởng lợi từ lần thứ hai hoặc thậm chí là lần thứ ba."} +{"idx": 1871138, "en": "OBJECTIVE To investigate the correlation of multiparametric magnetic resonance imaging targeted (TBx) and/or systematic prostate biopsy (SBx) in predicting the presence of clinically significant (cs) prostate cancer (PCa) in radical prostatectomy (RP) specimens. Concordance of mpMRI and RP specimen lesions was also investigated in terms of tumor localization and histopathological features. METHODS A total of 70 male patients with PCa and treated with robot-assisted RP were included in this study between January 2016 and December 2019. All patients underwent mpMRI-TBx and concomitant SBx. Suspicious lesions on mpMRI were scored according to Prostate Imaging-Reporting and Data System version 2 (PI-RADS) criteria. TBx was performed for all suspicious lesions with a PI-RADS score ≥3. RESULTS The median age was 67 (43-77) years. Presence of csPCa in prostatectomy specimens was missed by TBx and SBx specimens in 25.4% and 19.4% of the cases, respectively (P<.001, for each). Combination of both biopsy (CBx) results improved detection by missing only 4.5% of csPCa (P = .250). International Society of Urologic Pathology grade group concordance with RP specimens were 50%, 54.3% and 67.1% for SBx, TBx, and CBx, respectively. There was a statistically significant correlation in terms of tumor localization and histopathological features between prostatectomy specimens and the first 3 lesions, particularly for the index lesions. CONCLUSIONS CBx improved detection rate of csPCa. We propose TBx of 3 lesions with highest PI-RADS score(s) and a combination with SBx for the highest correlation with prostatectomy histopathology.", "gemini": "MỤC TIÊU Nghiên cứu mối tương quan của chụp cộng hưởng từ đa thông số có nhắm mục tiêu (TBx) và/hoặc sinh thiết tuyến tiền liệt có hệ thống (SBx) trong việc dự đoán sự hiện diện của ung thư tuyến tiền liệt (PCa) có ý nghĩa lâm sàng (cs) trong các mẫu cắt bỏ tuyến tiền liệt gốc (RP). Sự phù hợp của các tổn thương trên mpMRI và mẫu RP cũng được nghiên cứu về mặt định vị khối u và các đặc điểm mô học. PHƯƠNG PHÁP Tổng cộng 70 bệnh nhân nam bị PCa và được điều trị bằng phương pháp cắt bỏ tuyến tiền liệt hỗ trợ bằng robot đã được đưa vào nghiên cứu này trong khoảng thời gian từ tháng 1 năm 2016 đến tháng 12 năm 2019. Tất cả bệnh nhân đều được chụp mpMRI-TBx và SBx đồng thời. Các tổn thương nghi ngờ trên mpMRI được chấm điểm theo tiêu chí Hệ thống báo cáo và dữ liệu hình ảnh tuyến tiền liệt phiên bản 2 (PI-RADS). TBx được thực hiện cho tất cả các tổn thương nghi ngờ có điểm PI-RADS ≥3. KẾT QUẢ Độ tuổi trung bình là 67 (43-77) tuổi. Sự hiện diện của csPCa trong các mẫu cắt bỏ tuyến tiền liệt bị bỏ sót bởi các mẫu TBx và SBx trong 25,4% và 19,4% các trường hợp, tương ứng (P<.001, cho từng trường hợp). Kết hợp cả hai kết quả sinh thiết (CBx) đã cải thiện khả năng phát hiện bằng cách chỉ bỏ sót 4,5% csPCa (P = .250). Sự phù hợp của nhóm phân loại của Hiệp hội Bệnh học Tiết niệu Quốc tế với các mẫu RP lần lượt là 50%, 54,3% và 67,1% đối với SBx, TBx và CBx. Có một mối tương quan có ý nghĩa thống kê về mặt định vị khối u và các đặc điểm mô học giữa các mẫu cắt bỏ tuyến tiền liệt và 3 tổn thương đầu tiên, đặc biệt là đối với các tổn thương chỉ số. KẾT LUẬN CBx cải thiện tỷ lệ phát hiện csPCa. Chúng tôi đề xuất TBx của 3 tổn thương có điểm PI-RADS cao nhất và kết hợp với SBx để có mối tương quan cao nhất với mô bệnh học cắt bỏ tuyến tiền liệt."} +{"idx": 1871139, "en": "This narrative review examines associations of delay discounting, response inhibition, sensation-seeking, and urgency with adolescent problem substance use. Each of these processes is linked to adult substance use disorders, is associated with conditions linked to increased risk for adolescent problem substance use, and predicts problem substance use. Notably, all processes are linked to early life adversity (ELA) exposure and most appear to help explain links between ELA exposure and problem substance use. These findings are consistent with a growing body of literature indicating ELA interferes with the development of neural circuits crucial to cognitive functioning and emotion regulation. Further, developmental trajectories of these processes generally align with maturational imbalance hypotheses of adolescent risk. Ongoing and pending large longitudinal studies may be essential for better understanding how ELA and other influences shapes these processes and the role of these processes in risk for problem substance use in adolescence and beyond. Finally it is possible that risk-related processes may be useful metrics in the context of implementing and evaluating strategies to prevent problem substance use in adolescence.", "gemini": "Bài tổng quan tường thuật này xem xét các mối liên quan giữa việc giảm giá trì hoãn, ức chế đáp ứng, tìm kiếm cảm giác và tính cấp bách với việc sử dụng chất gây nghiện có vấn đề ở thanh thiếu niên. Mỗi quá trình này đều có liên quan đến các rối loạn sử dụng chất ở người lớn, có liên quan đến các tình trạng liên quan đến nguy cơ sử dụng chất gây nghiện có vấn đề ở thanh thiếu niên và dự đoán việc sử dụng chất gây nghiện có vấn đề. Đáng chú ý, tất cả các quá trình đều có liên quan đến việc tiếp xúc với nghịch cảnh thời thơ ấu (ELA) và hầu hết dường như giúp giải thích mối liên hệ giữa việc tiếp xúc với ELA và việc sử dụng chất gây nghiện có vấn đề. Những phát hiện này phù hợp với một lượng lớn tài liệu chỉ ra rằng ELA cản trở sự phát triển của các mạch thần kinh rất quan trọng đối với chức năng nhận thức và điều hòa cảm xúc. Hơn nữa, các quỹ đạo phát triển của các quá trình này thường phù hợp với các giả thuyết mất cân bằng trưởng thành về nguy cơ ở thanh thiếu niên. Các nghiên cứu theo chiều dọc lớn đang diễn ra và đang chờ xử lý có thể rất cần thiết để hiểu rõ hơn về cách ELA và các ảnh hưởng khác định hình các quá trình này và vai trò của các quá trình này trong nguy cơ sử dụng chất gây nghiện có vấn đề ở tuổi vị thành niên và sau này. Cuối cùng, có thể các quá trình liên quan đến rủi ro có thể là các số liệu hữu ích trong bối cảnh triển khai và đánh giá các chiến lược để ngăn ngừa việc sử dụng chất gây nghiện có vấn đề ở tuổi vị thành niên."} +{"idx": 1871140, "en": "Adolescence is a critical period of development with robust behavioral, morphological, hormonal, and neurochemical changes including changes in brain regions implicated in the reinforcing effects of drugs such as opioids. Here we examine the preclinical and, where appropriate complementary clinical literature, for the behavioral and neurological changes induced by adolescent opioid exposure/use and their long-term consequences during adulthood. Adolescent opioid exposure results in a widened biphasic shift in reinforcement with increased impact of positive rewarding aspects during initial use and profound negative reinforcement during adulthood. Females may have enhanced vulnerability due to fast onset of antinociceptive tolerance and reduced severity of somatic withdrawal symptoms during adolescence. Overall, adolescent opioid exposure, be it legally prescribed protracted intake or illicit consumption, results in significant and prolonged consequences of increased opioid reward concomitant with reduced analgesic efficacy and exacerbated somatic withdrawal severity during opioid use/exposure in adulthood. These findings are highly relevant to physicians, parents, law makers, and the general public as adolescent opioid exposure/misuse results in heightened risk for substance use disorders.", "gemini": "Tuổi vị thành niên là giai đoạn phát triển quan trọng với những thay đổi mạnh mẽ về hành vi, hình thái, nội tiết tố và thần kinh hóa học, bao gồm những thay đổi ở các vùng não liên quan đến tác dụng củng cố của thuốc như thuốc opioid. Ở đây, chúng tôi xem xét các tài liệu lâm sàng bổ sung tiền lâm sàng và thích hợp để biết những thay đổi về hành vi và thần kinh do tiếp xúc/sử dụng opioid ở tuổi vị thành niên và hậu quả lâu dài của chúng trong thời kỳ trưởng thành. Tiếp xúc với opioid ở tuổi vị thành niên dẫn đến sự thay đổi hai pha mở rộng trong sự củng cố với tác động gia tăng của các khía cạnh khen thưởng tích cực trong quá trình sử dụng ban đầu và sự củng cố tiêu cực sâu sắc trong thời kỳ trưởng thành. Phụ nữ có thể có khả năng dễ bị tổn thương hơn do tình trạng dung nạp giảm đau khởi phát nhanh và giảm mức độ nghiêm trọng của các triệu chứng cai thể xác trong thời kỳ vị thành niên. Nhìn chung, việc tiếp xúc với opioid ở tuổi vị thành niên, dù là dùng kéo dài theo đơn của bác sĩ hay sử dụng bất hợp pháp, đều dẫn đến những hậu quả đáng kể và kéo dài của việc tăng phần thưởng opioid cùng với hiệu quả giảm đau và tăng mức độ nghiêm trọng của tình trạng cai thể xác trong quá trình sử dụng/tiếp xúc với opioid ở tuổi trưởng thành. Những phát hiện này có liên quan cao đến các bác sĩ, phụ huynh, nhà lập pháp và công chúng vì việc tiếp xúc/sử dụng sai opioid ở tuổi vị thành niên dẫn đến nguy cơ mắc các rối loạn sử dụng chất cao hơn."} +{"idx": 1871141, "en": "Phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) are a group of small insects of great concern for Public Health. These dipterous are intensely studied worldwide due to their involvement in the transmission of several pathogens, mainly Leishmania spp. parasites. Nowadays, the molecular tools have been included in Phlebotomine sand flies studies and has shown to be powerful tools in bioecology studies of these dipterous. Thereby, when molecular approaches are employed, there is a great concern regarding the amount and quality of the DNA obtained for analysis. Here, seven methods of DNA extraction, between commercial kits and in house extraction protocols were evaluated. We considered measure of DNA concentration and purity ratios using a spectrophotometer to check the performance of each protocol. In addition, the quality evaluation of the DNA extracted was performed by endogenous gene PCR on samples. The results of the seven evaluated DNA extraction protocols and their implications are discussed.", "gemini": "Ruồi cát Phlebotomine (Diptera: Psychodidae: Phlebotominae) là một nhóm côn trùng nhỏ gây lo ngại lớn đối với Sức khỏe cộng đồng. Những loài hai cánh này được nghiên cứu chuyên sâu trên toàn thế giới do chúng liên quan đến việc truyền một số tác nhân gây bệnh, chủ yếu là ký sinh trùng Leishmania spp. Ngày nay, các công cụ phân tử đã được đưa vào các nghiên cứu về ruồi cát Phlebotomine và đã được chứng minh là những công cụ mạnh mẽ trong các nghiên cứu về sinh thái học của những loài hai cánh này. Do đó, khi các phương pháp tiếp cận phân tử được sử dụng, người ta rất quan tâm đến số lượng và chất lượng DNA thu được để phân tích. Ở đây, bảy phương pháp chiết xuất DNA, giữa các bộ dụng cụ thương mại và các giao thức chiết xuất tại nhà đã được đánh giá. Chúng tôi đã cân nhắc đến nồng độ DNA và tỷ lệ tinh khiết bằng cách sử dụng máy quang phổ để kiểm tra hiệu suất của từng giao thức. Ngoài ra, đánh giá chất lượng DNA được chiết xuất đã được thực hiện bằng PCR gen nội sinh trên các mẫu. Kết quả của bảy giao thức chiết xuất DNA được đánh giá và ý nghĩa của chúng được thảo luận."} +{"idx": 1871142, "en": "As many studies showed the spatial heterogeneity in the association between particulate matter (PM) air pollution and low birth weight (LBW), few studies focused on the variation of local associations at the national scale and related areal characteristics. This study aimed to explore different approaches to estimating local effects of PM with an aerodynamic diameter ≤10 μm (PM10) on LBW across 235 districts in South Korea, to investigate the spatial pattern of local associations, and to examine the relationship with local socio-demographic and environmental characteristics. LBW was identified in 5,692,650 mothers from birth certificate data for 2001-2013. We estimated individual annual-average concentrations of PM10 at centroids of mothers' residential districts by using a previously-validated prediction model. Then, we estimated district-specific odds ratios of LBW for PM10 using modified geographically weighted logistic regression. Here, we applied four approaches with different neighborhood definitions: the distance-based approach within 20- and 40-km bandwidth and the hybrid approach replacing with adjacent districts for urban districts <100 km2. In addition, we compared district-specific socioeconomic indicators and emission estimates across three groups of districts that showed significantly positive, no, and significantly negative associations. Medians of district-specific estimates of four approaches were similar to the global estimate and between each other. However, their variability differed with some unreasonably high estimates when a small distance was applied as the neighborhood definition, although spatial pattern was generally similar among the four. The hybrid approach based on the different neighborhood definition by urban and rural areas provided stable risk estimates. Higher risk districts in rural areas were found in more socioeconomically-deprived areas, whereas urban areas showed higher risk districts when their air pollution emissions were higher. Our approach and findings will help identify high risk areas and enhance understanding of geographic determinants.", "gemini": "Vì nhiều nghiên cứu đã chỉ ra tính không đồng nhất về mặt không gian trong mối liên hệ giữa ô nhiễm không khí dạng hạt (PM) và trẻ nhẹ cân (LBW), một số ít nghiên cứu tập trung vào sự thay đổi của các mối liên hệ cục bộ ở quy mô quốc gia và các đặc điểm diện tích liên quan. Nghiên cứu này nhằm khám phá các cách tiếp cận khác nhau để ước tính các tác động cục bộ của PM có đường kính khí động học ≤10 μm (PM10) đối với LBW trên 235 quận tại Hàn Quốc, để điều tra mô hình không gian của các mối liên hệ cục bộ và để kiểm tra mối quan hệ với các đặc điểm xã hội-dân số và môi trường cục bộ. LBW được xác định ở 5.692.650 bà mẹ từ dữ liệu giấy khai sinh từ năm 2001-2013. Chúng tôi ước tính nồng độ trung bình hàng năm của PM10 tại các điểm trọng tâm của các quận nơi cư trú của bà mẹ bằng cách sử dụng mô hình dự đoán đã được xác thực trước đó. Sau đó, chúng tôi ước tính tỷ lệ cược LBW riêng của từng quận đối với PM10 bằng cách sử dụng hồi quy logistic có trọng số địa lý đã sửa đổi. Ở đây, chúng tôi áp dụng bốn cách tiếp cận với các định nghĩa về khu vực lân cận khác nhau: cách tiếp cận dựa trên khoảng cách trong phạm vi băng thông 20 và 40 km và cách tiếp cận kết hợp thay thế bằng các quận liền kề đối với các quận đô thị <100 km2. Ngoài ra, chúng tôi đã so sánh các chỉ số kinh tế xã hội riêng của từng quận và ước tính phát thải trên ba nhóm quận có mối liên hệ tích cực đáng kể, không có và tiêu cực đáng kể. Các giá trị trung bình của các ước tính riêng của từng quận của bốn cách tiếp cận tương tự như ước tính toàn cầu và giữa mỗi cách tiếp cận. Tuy nhiên, độ biến thiên của chúng khác nhau với một số ước tính cao không hợp lý khi áp dụng khoảng cách nhỏ làm định nghĩa khu vực lân cận, mặc dù mô hình không gian nhìn chung giống nhau giữa bốn cách tiếp cận. Cách tiếp cận kết hợp dựa trên định nghĩa khu vực lân cận khác nhau theo khu vực thành thị và nông thôn cung cấp ước tính rủi ro ổn định. Các quận có nguy cơ cao hơn ở các vùng nông thôn được tìm thấy ở các khu vực nghèo đói hơn về mặt kinh tế xã hội, trong khi các khu vực đô thị cho thấy các quận có nguy cơ cao hơn khi lượng phát thải ô nhiễm không khí của họ cao hơn. Cách tiếp cận và phát hiện của chúng tôi sẽ giúp xác định các khu vực có nguy cơ cao và nâng cao hiểu biết về các yếu tố quyết định về mặt địa lý."} +{"idx": 1871143, "en": "BACKGROUND perfluoroalkylated substances (PFAS) are highly persistent chemicals that are able to alter the human metabolism - potentially via disruption of cell signaling pathways mediated by adipokines. Both adiponectin and leptin are influenced by and exert influence on energy storage and energy expenditure, wherefore associations between PFAS and adipokines may be mediated by fitness and fat mass. OBJECTIVES the aim of this cross-sectional study was to investigate the association between childhood exposure to PFAS and adipokines (adiponectin and leptin), while considering associations between PFAS and children's level of fitness, physical activity and fat mass to elucidate potential mediation by fitness, physical activity and fat mass. METHODS 9-year old children from Danish public schools were recruited in the European Youth Heart Study in 1997. For this study only children with valid measures on PFAS (PFOS, PFOA, PFNA, PFDA and PFHxS), adipokines (adiponectin and leptin), fitness, fat mass and co-variates (parity and maternal income) were included (N = 242). Multiple linear regression models with and without conditioning and causal mediation analysis were applied. RESULTS this study found inverse associations between PFOA, PFDA and PFHxS and leptin. PFOA was positively associated with adiponectin, whereas PFHxS was inversely associated with adiponectin in boys. Latter association seemed to be mediated by fat mass. Associations with leptin showed indirect effects of fitness and fat mass but were unable to demonstrate significant mediation. Neither PFOS nor PFNA were associated with the outcome. DISCUSSION these results may indicate a favorable leptin profile with increasing PFAS, although the results could be driven by residual negative confounding from socio-economic factors and mediation by fitness and fat mass.", "gemini": "HOÀN CẢNH các chất perfluoroalkyl (PFAS) là các hóa chất rất bền có khả năng làm thay đổi quá trình trao đổi chất của con người - có khả năng thông qua sự phá vỡ các con đường truyền tín hiệu tế bào được trung gian bởi các adipokine. Cả adiponectin và leptin đều bị ảnh hưởng và có ảnh hưởng đến quá trình dự trữ năng lượng và tiêu hao năng lượng, do đó mối liên quan giữa PFAS và adipokine có thể được trung gian bởi thể lực và khối lượng mỡ. MỤC TIÊU mục đích của nghiên cứu cắt ngang này là điều tra mối liên quan giữa việc tiếp xúc với PFAS ở thời thơ ấu và adipokine (adiponectin và leptin), đồng thời xem xét mối liên quan giữa PFAS và mức độ thể lực, hoạt động thể chất và khối lượng mỡ của trẻ em để làm sáng tỏ sự trung gian tiềm ẩn của thể lực, hoạt động thể chất và khối lượng mỡ. PHƯƠNG PHÁP trẻ em 9 tuổi từ các trường công lập Đan Mạch đã được tuyển dụng trong Nghiên cứu tim mạch ở thanh thiếu niên châu Âu năm 1997. Đối với nghiên cứu này, chỉ những trẻ có số đo hợp lệ về PFAS (PFOS, PFOA, PFNA, PFDA và PFHxS), adipokine (adiponectin và leptin), thể lực, khối lượng mỡ và các biến đồng biến (số con và thu nhập của mẹ) mới được đưa vào (N = 242). Các mô hình hồi quy tuyến tính đa biến có và không có điều kiện và phân tích trung gian nhân quả đã được áp dụng. KẾT QUẢ nghiên cứu này phát hiện ra mối liên quan nghịch đảo giữa PFOA, PFDA và PFHxS và leptin. PFOA có liên quan tích cực với adiponectin, trong khi PFHxS có liên quan nghịch đảo với adiponectin ở nam. Mối liên quan sau có vẻ như được trung gian bởi khối lượng mỡ. Mối liên quan với leptin cho thấy các tác động gián tiếp của thể lực và khối lượng mỡ nhưng không thể chứng minh được sự trung gian đáng kể. Cả PFOS và PFNA đều không liên quan đến kết quả. THẢO LUẬN những kết quả này có thể chỉ ra một cấu hình leptin thuận lợi khi PFAS tăng, mặc dù các kết quả có thể bị thúc đẩy bởi sự nhiễu loạn tiêu cực còn sót lại từ các yếu tố kinh tế xã hội và sự trung gian của thể lực và khối lượng mỡ."} +{"idx": 1871144, "en": "To reduce the high operational costs of water treatment because of membrane biofouling, next-generation materials are being developed to counteract microbial growth. These modern anti-biofouling strategies are based on new membrane materials or membrane surface modifications. In this study, antimicrobial films comprising rGO, rGO-CuO, rGO-Ag, and rGO-CuO-Ag were synthesized, evaluated, and tested for potential biofouling control using Pseudomonas aeruginosa PAO1 as the model bacterium. The combined rGO-CuO-Ag film displayed enhanced reduction (10-log reduction) in biofouling in comparison to the rGO film (control), followed by the rGO-Ag film (8-log reduction) and rGO-CuO film (0-log reduction). This demonstrated that the use of mixed antimicrobial agents is more effective in reducing biofouling than that of a single agent. The rGO-CuO-Ag film exhibited consistent, controlled, and moderate release of silver (Ag) ions. The release of Ag ions produced a long-lasting antimicrobial effect. These results underscore the potential applications of combined antimicrobial surface-based agents in practice and further research.", "gemini": "Để giảm chi phí vận hành cao của xử lý nước do màng sinh học, các vật liệu thế hệ tiếp theo đang được phát triển để chống lại sự phát triển của vi khuẩn. Các chiến lược chống bám bẩn hiện đại này dựa trên vật liệu màng mới hoặc sửa đổi bề mặt màng. Trong nghiên cứu này, các màng kháng khuẩn bao gồm rGO, rGO-CuO, rGO-Ag và rGO-CuO-Ag đã được tổng hợp, đánh giá và thử nghiệm để kiểm soát khả năng bám bẩn sinh học tiềm ẩn bằng cách sử dụng Pseudomonas aeruginosa PAO1 làm vi khuẩn mô hình. Màng rGO-CuO-Ag kết hợp cho thấy khả năng giảm đáng kể (giảm 10 log) sự bám bẩn sinh học so với màng rGO (kiểm soát), tiếp theo là màng rGO-Ag (giảm 8 log) và màng rGO-CuO (giảm 0 log). Điều này chứng minh rằng việc sử dụng các tác nhân kháng khuẩn hỗn hợp có hiệu quả hơn trong việc giảm sự bám bẩn sinh học so với một tác nhân duy nhất. Màng rGO-CuO-Ag thể hiện sự giải phóng ion bạc (Ag) ổn định, có kiểm soát và vừa phải. Việc giải phóng các ion Ag tạo ra tác dụng kháng khuẩn lâu dài. Những kết quả này nhấn mạnh các ứng dụng tiềm năng của các tác nhân kết hợp dựa trên bề mặt kháng khuẩn trong thực tế và nghiên cứu sâu hơn."} +{"idx": 1871145, "en": "Gold nanorods (AuNRs) are promising nanoscale materials for several technological and biomedical applications. The physicochemical properties of AuNRs, including size, shape and surface features, are crucial factors affecting their cytotoxicity. In this study, we investigated the effects of different aspect ratios of AuNRs (1.90, 2.35, 3.25 and 3.50) at concentrations of 2 and 10 μg mL-1 on their cytotoxicity and cellular uptake in green algae Raphidocelis subcaptata. The experiment was performed in oligotrophic freshwater medium in a growth chamber with constant agitation of 80 rpm under controlled conditions (120 μEm-2s-1 illumination; 12:12h light dark cycle and constant temperature of 22 ± 2 °C). The algal growth was monitored daily for 96 h via electronic absorbance scanning at 600-750 nm. Oxidative stress, cell viability and autofluorescence were evaluated using a flow cytometer. Oxidative stress quantified by loading cultures with the fluorescent dye 2', 7'-dichlorofluorescein diacetate. To assess algal cell viability, propidium iodide was selected as the fluorescent probe. Our results indicated that the aspect ratio of AuNRs mediates their biological effects in green algae R. subcaptata. A positive correlation between oxidative stress and increase of aspect ratio was found at concentration of 10 μg mL-1. Higher cytotoxicity and mortality were observed for algae incubated with higher aspect ratios AuNRs (3.50). These findings may be useful to understand the impact of the AuNRs in aquatic environments, contributing to ecosystem management and nanomaterials regulation.", "gemini": "Các nanorod vàng (AuNRs) là vật liệu ở quy mô nano đầy hứa hẹn cho một số ứng dụng công nghệ và y sinh học. Các tính chất lý hóa của AuNRs, bao gồm kích thước, hình dạng và đặc điểm bề mặt, là những yếu tố quan trọng ảnh hưởng đến độc tính tế bào của chúng. Trong nghiên cứu này, chúng tôi đã điều tra các tác động của các tỷ lệ khung hình khác nhau của AuNRs (1,90, 2,35, 3,25 và 3,50) ở nồng độ 2 và 10 μg mL-1 đối với độc tính tế bào và khả năng hấp thụ vào tế bào ở tảo lục Raphidocelis subcaptata. Thí nghiệm được thực hiện trong môi trường nước ngọt nghèo dinh dưỡng trong buồng tăng trưởng với sự khuấy liên tục 80 vòng / phút trong điều kiện được kiểm soát (chiếu sáng 120 μEm-2s-1; chu kỳ sáng tối 12: 12 giờ và nhiệt độ không đổi 22 ± 2 ° C). Sự phát triển của tảo được theo dõi hàng ngày trong 96 giờ thông qua quét hấp thụ điện tử ở bước sóng 600-750 nm. Căng thẳng oxy hóa, khả năng sống của tế bào và tự phát huỳnh quang được đánh giá bằng máy đo lưu lượng tế bào. Căng thẳng oxy hóa được định lượng bằng cách nạp các nền văn hóa với thuốc nhuộm huỳnh quang 2', 7'-dichlorofluorescein diacetate. Để đánh giá khả năng sống của tế bào tảo, propidium iodide được chọn làm đầu dò huỳnh quang. Kết quả của chúng tôi chỉ ra rằng tỷ lệ khung hình của AuNRs có tác động đến các hiệu ứng sinh học của chúng trong tảo lục R. subcaptata. Đã tìm thấy mối tương quan tích cực giữa căng thẳng oxy hóa và sự gia tăng tỷ lệ khung hình ở nồng độ 10 μg mL-1. Độc tính tế bào và tỷ lệ tử vong cao hơn được quan sát thấy ở tảo được ủ với AuNRs có tỷ lệ khung hình cao hơn (3,50). Những phát hiện này có thể hữu ích để hiểu tác động của AuNRs trong môi trường nước, góp phần vào việc quản lý hệ sinh thái và quy định vật liệu nano."} +{"idx": 1871146, "en": "Silicone can present a challenge during the development of a biologics drug product particularly in pre-filled syringe (PFS). Due to silicone related challenges, substantial changes in components and manufacturing of the PFS are being sought. Cross-linking of the silicone being one of them, can help reduce mobilization of the silicone into drug product whilst retaining its functionality of lubrication during injection. In this work, we systematically compare the stability of a fusion protein and monoclonal antibody formulation in conventionally siliconized and cross-linked siliconized PFS available from commercial manufacturers. The two types of syringes did not influence the aggregation profile of proteins as determined by HP-SEC. Compared to conventionally siliconized PFS, a cross-linked siliconized PFS can have a favorable or indifferent impact (depending on vendor) on the sub-visible particles profile as assessed by light obscuration and microflow imaging. The different PFS after 24 months of long-term storage showed comparable functionality attributes like break-loose/gliding force and silicone oil distribution. Cross-linked siliconized PFS can offer an incremental advantage over conventionally siliconized PFS for the moderately concentrated protein solutions, however the differences in the quality of these PFS amongst manufacturers is an important aspect that needs to be considered as shown in this study.", "gemini": "Silicone có thể gây ra thách thức trong quá trình phát triển một sản phẩm thuốc sinh học, đặc biệt là trong ống tiêm đã nạp sẵn (PFS). Do những thách thức liên quan đến silicone, những thay đổi đáng kể về thành phần và sản xuất của PFS đang được tìm kiếm. Liên kết chéo của silicone là một trong số đó, có thể giúp giảm sự di động của silicone vào sản phẩm thuốc trong khi vẫn giữ được chức năng bôi trơn của nó trong quá trình tiêm. Trong công trình này, chúng tôi so sánh một cách có hệ thống tính ổn định của một protein hòa hợp và công thức kháng thể đơn dòng trong PFS silicon hóa thông thường và PFS silicon hóa liên kết chéo có sẵn từ các nhà sản xuất thương mại. Hai loại ống tiêm này không ảnh hưởng đến cấu hình kết tập của protein như được xác định bởi HP-SEC. So với PFS silicon hóa thông thường, PFS silicon hóa liên kết chéo có thể có tác động thuận lợi hoặc không ảnh hưởng (tùy thuộc vào nhà cung cấp) đến cấu hình hạt dưới mức nhìn thấy như được đánh giá bằng phương pháp che khuất ánh sáng và hình ảnh vi dòng chảy. Các PFS khác nhau sau 24 tháng bảo quản dài hạn cho thấy các thuộc tính chức năng có thể so sánh được như lực bứt ra/trượt và phân phối dầu silicone. PFS silicon hóa liên kết chéo có thể mang lại lợi thế gia tăng so với PFS silicon hóa thông thường đối với các dung dịch protein có nồng độ trung bình, tuy nhiên sự khác biệt về chất lượng của các PFS này giữa các nhà sản xuất là một khía cạnh quan trọng cần được xem xét như đã trình bày trong nghiên cứu này."} +{"idx": 1871147, "en": "In this commentary I briefly discuss the term advanced therapy medicinal products (ATMPs). The last two words, medicinal products, correctly indicate that we are dealing with medicines. However, oftentimes ATMPs and products within the ATMP family are erroneously called therapies, which may raise confusion, as illustrated with some examples, and may lead to ignorance of the importance of pharmaceutical product quality. The first two words, advanced therapy, are arguable as well, because they do not accurately describe the products involved and advanced is a temporal description of therapies, many of which may not (yet) be advanced at all. It is recommended to avoid such confusing wording and instead call medicines by their proper name.", "gemini": "Trong bình luận này, tôi thảo luận ngắn gọn về thuật ngữ các sản phẩm thuốc điều trị tiên tiến (ATMP). Hai từ cuối cùng, sản phẩm thuốc, chỉ ra đúng rằng chúng ta đang nói đến thuốc. Tuy nhiên, thường thì ATMP và các sản phẩm trong họ ATMP lại bị gọi nhầm là liệu pháp, điều này có thể gây nhầm lẫn, như được minh họa bằng một số ví dụ và có thể dẫn đến sự thiếu hiểu biết về tầm quan trọng của chất lượng sản phẩm dược phẩm. Hai từ đầu tiên, liệu pháp tiên tiến, cũng có thể tranh luận, bởi vì chúng không mô tả chính xác các sản phẩm liên quan và tiên tiến là mô tả tạm thời về các liệu pháp, nhiều liệu pháp trong số đó có thể (chưa) tiên tiến chút nào. Nên tránh cách diễn đạt gây nhầm lẫn như vậy và thay vào đó gọi thuốc bằng tên riêng của chúng."} +{"idx": 1871148, "en": "RT001 is the di-deutero isotopologue of linoleic acid ethyl ester (D2-LA). Resistance to oxidative damage at the carbon-deuterium bond depends upon the concentration of D2-LA as a percentage of total LA. We report here on the plasma and red cell (RBC) pharmacokinetics (PK) of D2-LA, and its metabolite 13,13-D2-arachidonic acid (D2-AA), in patients with multiple neurodegenerative diseases (total of 59 participants). In Friedreich's ataxia patients, D2-LA was absorbed and transported similarly to dietary LA, peaking at about 6 h after oral dosing. Plasma D2-LA concentrations approached steady state after 28 days of dosing. After 6 months of daily dosing in subjects with other disorders, D2-LA and D2-AA levels were at or above the 20% of total (D2-LA/total LA, or D2-AA/total AA) therapeutic targets for most subjects. We conclude that chronic dosing of RT001 and associated dietary guidance can be maintained over many months to achieve target plasma and RBC levels, forming a basis for therapeutic dosing across a broad range of conditions. RT001 has been safe and well-tolerated in 59 different participants treated across 10 different neurodegenerative diseases in multiple clinical trials for up to 36 months with no significant drug related adverse events limiting use.", "gemini": "RT001 là đồng vị đơteri của este ethyl axit linoleic (D2-LA). Khả năng chống lại tổn thương oxy hóa ở liên kết cacbon-đơteri phụ thuộc vào nồng độ D2-LA dưới dạng phần trăm của tổng LA. Chúng tôi báo cáo ở đây về dược động học trong huyết tương và hồng cầu (RBC) (PK) của D2-LA và chất chuyển hóa của nó là axit arachidonic 13,13-D2 (D2-AA), ở những bệnh nhân mắc nhiều bệnh thoái hóa thần kinh (tổng cộng 59 người tham gia). Ở những bệnh nhân mắc chứng mất điều hòa Friedreich, D2-LA được hấp thụ và vận chuyển tương tự như LA trong chế độ ăn, đạt đỉnh sau khoảng 6 giờ sau khi uống thuốc. Nồng độ D2-LA trong huyết tương đạt trạng thái ổn định sau 28 ngày dùng thuốc. Sau 6 tháng dùng thuốc hàng ngày ở những đối tượng mắc các rối loạn khác, nồng độ D2-LA và D2-AA đạt hoặc vượt quá 20% tổng số (D2-LA/tổng LA hoặc D2-AA/tổng AA) mục tiêu điều trị đối với hầu hết các đối tượng. Chúng tôi kết luận rằng việc dùng RT001 mạn tính và hướng dẫn chế độ ăn liên quan có thể được duy trì trong nhiều tháng để đạt được nồng độ mục tiêu trong huyết tương và RBC, tạo cơ sở cho liều điều trị trên nhiều tình trạng khác nhau. RT001 đã an toàn và được dung nạp tốt ở 59 người tham gia khác nhau được điều trị trong 10 bệnh thoái hóa thần kinh khác nhau trong nhiều thử nghiệm lâm sàng trong tối đa 36 tháng mà không có tác dụng phụ đáng kể nào liên quan đến thuốc hạn chế việc sử dụng."} +{"idx": 1871149, "en": "How an animal establishes its body axis is a fundamental question in developmental biology. The freshwater cnidarian Hydra is an attractive model for studying axis formation because it is radially symmetric, with a single oral-aboral axis. It was recently proposed that the orientation of the new body axis in a regenerating Hydra polyp is determined by the oral-aboral orientation of the actin-myosin contractile processes (myonemes) in the animal's outer epithelial layer. However, it remained unclear how the oral-aboral polarity of the body axis would be defined. As Wnt signaling is known to control axis polarity in Hydra and bilaterians, we hypothesized that it plays a role in axis formation during regeneration of Hydra tissue pieces. We tested this hypothesis using pharmacological perturbations and novel grafting experiments to set Wnt signaling and myoneme orientation perpendicular to each other to determine which controls axis formation. Our results demonstrate that Wnt signaling is the dominant encoder of axis orientation and polarity, in line with its conserved role in axial patterning.", "gemini": "Cách một loài động vật thiết lập trục cơ thể của mình là một câu hỏi cơ bản trong sinh học phát triển. Hydra nước ngọt là một mô hình hấp dẫn để nghiên cứu sự hình thành trục vì nó có đối xứng xuyên tâm, với một trục miệng-lỗ hậu môn duy nhất. Gần đây có đề xuất rằng hướng của trục cơ thể mới trong một polyp Hydra tái sinh được xác định bởi hướng miệng-lỗ hậu môn của các quá trình co cơ actin-myosin (myonem) trong lớp biểu mô ngoài của động vật. Tuy nhiên, vẫn chưa rõ cách xác định cực miệng-lỗ hậu môn của trục cơ thể. Vì tín hiệu Wnt được biết là kiểm soát cực trục ở Hydra và động vật hai bên, chúng tôi đưa ra giả thuyết rằng nó đóng vai trò trong quá trình hình thành trục trong quá trình tái tạo các mảnh mô Hydra. Chúng tôi đã kiểm tra giả thuyết này bằng cách sử dụng các nhiễu loạn dược lý và các thí nghiệm ghép mới để đặt tín hiệu Wnt và hướng myonem vuông góc với nhau để xác định yếu tố nào kiểm soát sự hình thành trục. Kết quả của chúng tôi chứng minh rằng tín hiệu Wnt là bộ mã hóa trội của hướng trục và cực, phù hợp với vai trò bảo tồn của nó trong quá trình tạo mẫu trục."} +{"idx": 1871150, "en": "Alcohol misuse and dependence is a widespread health problem. The central nucleus of the amygdala (CeA) plays important roles in both the anxiety associated with alcohol (ethanol) dependence and the increased alcohol intake that is observed during withdrawal in dependent animals. We and others have shown the essential involvement of the corticotropin releasing factor (CRF) system in alcohol's synaptic effects on the CeA and in the development of ethanol dependence. Another system that has been shown to be critically involved in the molecular underpinnings of alcohol dependence is the norepinephrine (NE) system originating in the locus coeruleus. Both the CRF and NE systems act in concert to facilitate a stress response: central amygdalar afferents release CRF in the locus coeruleus promoting widespread release of NE. In this study, we are the first to use fast-scan cyclic voltammetry to classify local electrically-evoked NE release in the CeA and to determine if acute alcohol and CRF modulate it. Evoked NE release is action potential dependent, is abolished after depletion of monoaminergic vesicles, differs pharmacologically from dopamine release, is insensitive to acute alcohol, and decreases in response to locally applied CRF. Taken together, these results indicate that NE release in the CeA is released canonically in a vesicular-dependent manner, and that while acute alcohol does not directly alter NE release, CRF decreases it. Our results suggest that CRF acts locally on NE terminals as negative feedback and potentially prevents hyperactivation of the CRF-norepinephrine stress pathway.", "gemini": "Lạm dụng và phụ thuộc rượu là một vấn đề sức khỏe phổ biến. Hạch trung tâm của hạnh nhân (CeA) đóng vai trò quan trọng trong cả sự lo lắng liên quan đến chứng nghiện rượu (ethanol) và lượng rượu tăng lên được quan sát thấy trong quá trình cai ở những động vật nghiện. Chúng tôi và những người khác đã chỉ ra sự liên quan thiết yếu của hệ thống giải phóng yếu tố giải phóng corticotropin (CRF) trong các tác động của rượu lên synap ở CeA và trong quá trình phát triển chứng nghiện ethanol. Một hệ thống khác được chứng minh là có liên quan quan trọng đến nền tảng phân tử của chứng nghiện rượu là hệ thống norepinephrin (NE) có nguồn gốc từ locus coeruleus. Cả hệ thống CRF và NE đều hoạt động phối hợp để tạo điều kiện cho phản ứng căng thẳng: các sợi hướng tâm amygdalar trung tâm giải phóng CRF trong locus coeruleus thúc đẩy giải phóng NE rộng rãi. Trong nghiên cứu này, chúng tôi là những người đầu tiên sử dụng phương pháp đo điện thế vòng quét nhanh để phân loại quá trình giải phóng NE do điện gây ra tại chỗ ở CeA và xác định xem rượu cấp tính và CRF có điều chế nó hay không. Quá trình giải phóng NE được gây ra phụ thuộc vào tiềm hoạt động, bị xóa bỏ sau khi các túi chứa monoamin cạn kiệt, khác biệt về mặt dược lý so với quá trình giải phóng dopamine, không nhạy cảm với rượu cấp tính và giảm khi đáp ứng với CRF được áp dụng tại chỗ. Kết hợp lại, những kết quả này chỉ ra rằng quá trình giải phóng NE ở CeA được giải phóng theo cách cổ điển theo cách phụ thuộc vào túi và trong khi rượu cấp tính không trực tiếp làm thay đổi quá trình giải phóng NE, CRF làm giảm nó. Kết quả của chúng tôi cho thấy CRF hoạt động cục bộ trên các đầu mút NE như phản hồi tiêu cực và có khả năng ngăn ngừa sự hoạt hóa quá mức của con đường căng thẳng CRF-norepinephrin."} +{"idx": 1871151, "en": "In the presence of the novel Coronavirus Disease (COVID-19) and other new viral agents, one of the fundamental problems in science is the evaluation of environmental and social weaknesses of cities/regions to the exposure of infectious diseases for preventing and/or containing new COVID-19 outbreaks and the diffusion of other viral agents that generate a negative impact on public health and economy of countries. The current monitoring of transmission dynamics of infectious diseases is mainly based on reproduction number (R0) and fatality rates. However, this approach is a real-time monitoring of transmission dynamics for mitigating the numbers of COVID-19 related infected individuals and deaths. Reproduction number does not provide information to cope with future epidemics or pandemics. The main goal of this study is to propose the Index c (as contagions) that quantifies, ex-ante, the environmental risk of exposure of cities/regions to future epidemics of the COVID-19 and similar vital agents. This Index c synthetizes environmental, demographic, climatological and health risk factors of cities/regions that indicate their exposure to infectious diseases. Index c has a range from 1 (environmental and social weakness of urban areas leading to high levels of exposure to infectious diseases) to 0 (environment that reduces the risk of exposure to infectious diseases in society). The statistical evidence here, applied on case study of Italy, seems in general to support the predictive capacity of the Index c as a particularly simple but superior indicator in detecting the global correlation between potential risk of exposure of cities/regions to infectious diseases and actual risk given by infected individuals and deaths of the COVID-19. The Index c can support a proactive environmental strategy to help policymakers to prevent future pandemics similar to the COVID-19.", "gemini": "Với sự xuất hiện của Bệnh do virus Corona mới (COVID-19) và các tác nhân vi-rút mới khác, một trong những vấn đề cơ bản trong khoa học là đánh giá các điểm yếu về môi trường và xã hội của các thành phố/khu vực đối với sự phơi nhiễm với các bệnh truyền nhiễm để ngăn ngừa và/hoặc ngăn chặn các ổ dịch COVID-19 mới và sự lây lan của các tác nhân vi-rút khác gây ra tác động tiêu cực đến sức khỏe cộng đồng và nền kinh tế của các quốc gia. Việc theo dõi động lực lây truyền của các bệnh truyền nhiễm hiện nay chủ yếu dựa vào số sinh sản (R0) và tỷ lệ tử vong. Tuy nhiên, cách tiếp cận này là theo dõi thời gian thực về động lực lây truyền để giảm số lượng cá nhân bị nhiễm và tử vong liên quan đến COVID-19. Số sinh sản không cung cấp thông tin để đối phó với các dịch bệnh hoặc đại dịch trong tương lai. Mục tiêu chính của nghiên cứu này là đề xuất Chỉ số c (là các bệnh truyền nhiễm) định lượng trước rủi ro về môi trường khi các thành phố/khu vực tiếp xúc với các dịch bệnh COVID-19 trong tương lai và các tác nhân quan trọng tương tự. Chỉ số c này tổng hợp các yếu tố rủi ro về môi trường, nhân khẩu học, khí hậu học và sức khỏe của các thành phố/khu vực cho biết mức độ tiếp xúc của họ với các bệnh truyền nhiễm. Chỉ số c có phạm vi từ 1 (điểm yếu về môi trường và xã hội của các khu vực đô thị dẫn đến mức độ tiếp xúc cao với các bệnh truyền nhiễm) đến 0 (môi trường làm giảm nguy cơ tiếp xúc với các bệnh truyền nhiễm trong xã hội). Bằng chứng thống kê ở đây, được áp dụng trên trường hợp nghiên cứu của Ý, nhìn chung có vẻ ủng hộ khả năng dự đoán của Chỉ số c như một chỉ số đặc biệt đơn giản nhưng vượt trội trong việc phát hiện mối tương quan toàn cầu giữa nguy cơ tiếp xúc tiềm ẩn của các thành phố/khu vực với các bệnh truyền nhiễm và nguy cơ thực tế do những người bị nhiễm và tử vong do COVID-19. Chỉ số c có thể hỗ trợ một chiến lược môi trường chủ động để giúp các nhà hoạch định chính sách ngăn ngừa các đại dịch trong tương lai tương tự như COVID-19."} +{"idx": 1871152, "en": "Single particle tracking (SPT) is a robust technique to monitor single-molecule behaviors in living cells directly. By this approach, we can uncover the potential biological significance of particle dynamics by statistically characterizing individual molecular behaviors. SPT provides valuable information at the single-molecule level, that could be obscured by simple averaging that is inherent to conventional ensemble measurements. Here, we give a brief introduction to SPT including the commonly used optical implementations, fluorescence labeling strategies, and data analysis methods. We then focus on how SPT has been harnessed to decipher myocardial function. In this context, SPT has provided novel insight into the lateral diffusion of signal receptors and ion channels, the dynamic organization of cardiac nanodomains, subunit composition and stoichiometry of cardiac ion channels, myosin movement along actin filaments, the kinetic features of transcription factors involved in cardiac remodeling, and the intercellular communication by nanotubes. Finally, we speculate on the prospects and challenges of applying SPT to future questions regarding cellular cardiac physiology using SPT.", "gemini": "Theo dõi hạt đơn (SPT) là một kỹ thuật mạnh mẽ để theo dõi trực tiếp các hành vi của phân tử đơn trong các tế bào sống. Với phương pháp này, chúng ta có thể khám phá ý nghĩa sinh học tiềm tàng của động lực hạt bằng cách mô tả thống kê các hành vi phân tử riêng lẻ. SPT cung cấp thông tin có giá trị ở cấp độ phân tử đơn, có thể bị che khuất bởi việc tính trung bình đơn giản vốn có trong các phép đo tổng hợp thông thường. Ở đây, chúng tôi giới thiệu tóm tắt về SPT bao gồm các triển khai quang học thường được sử dụng, các chiến lược đánh dấu huỳnh quang và các phương pháp phân tích dữ liệu. Sau đó, chúng tôi tập trung vào cách SPT đã được khai thác để giải mã chức năng cơ tim. Trong bối cảnh này, SPT đã cung cấp cái nhìn sâu sắc mới về sự khuếch tán theo chiều ngang của các thụ thể tín hiệu và kênh ion, tổ chức động của các miền nano tim, thành phần và tỷ lệ lượng chất của các kênh ion tim, chuyển động myosin dọc theo các sợi actin, các đặc điểm động học của các yếu tố phiên mã liên quan đến việc tái tạo tim và liên lạc giữa các tế bào bằng ống nano. Cuối cùng, chúng tôi suy đoán về triển vọng và thách thức của việc áp dụng SPT cho các câu hỏi trong tương lai liên quan đến sinh lý tim tế bào bằng cách sử dụng SPT."} +{"idx": 1871153, "en": "The burgeoning field of single-cell transcriptomics augments our ability to scrutinize organ systems at unprecedented resolutions. Single-cell RNA sequencing (scRNA-seq) and analytical techniques have shed light on the cellular heterogeneity, developmental trajectories, intercellular communications of the cardiac system, and thus contributed much to the understanding of cardiac development, homeostasis and disorders. Although generalized protocols are well established for scRNA-seq pipelines, customized sample preparation, quality control, and data interpretation are still needed in cardiac research. In this article, we highlight major steps that impact data quality in scRNA-seq experiments, with particular focus on sample and data processing of cardiomyocytes. We also summarize popular applications of scRNA-seq, outlining general tools, caveats and examples in cardiac research.", "gemini": "Lĩnh vực phiên mã học tế bào đơn đang phát triển mạnh mẽ giúp tăng cường khả năng xem xét các hệ thống cơ quan ở độ phân giải chưa từng có. Trình tự RNA tế bào đơn (scRNA-seq) và các kỹ thuật phân tích đã làm sáng tỏ tính không đồng nhất của tế bào, quá trình phát triển, thông tin liên lạc giữa các tế bào của hệ thống tim và do đó đóng góp rất nhiều vào việc hiểu biết về sự phát triển, cân bằng nội môi và các rối loạn của tim. Mặc dù các giao thức tổng quát được thiết lập tốt cho các quy trình scRNA-seq, nhưng vẫn cần chuẩn bị mẫu tùy chỉnh, kiểm soát chất lượng và giải thích dữ liệu trong nghiên cứu về tim. Trong bài viết này, chúng tôi nêu bật các bước chính ảnh hưởng đến chất lượng dữ liệu trong các thí nghiệm scRNA-seq, đặc biệt tập trung vào việc xử lý mẫu và dữ liệu của tế bào cơ tim. Chúng tôi cũng tóm tắt các ứng dụng phổ biến của scRNA-seq, phác thảo các công cụ chung, cảnh báo và ví dụ trong nghiên cứu về tim."} +{"idx": 1871154, "en": "We characterized and compared the venom protein profiles of Hydrophis curtus (synonyms: Lapemis hardwickii, Lapemis curtus and Hydrophis hardwickii) and Hydrophis cyanocinctus, the two representatives of medically important venomous sea snakes in Chinese waters using proteomic approaches. A total of 47 and 38 putative toxins were identified in H. curtus venom (HcuV) and H. cyanocinctus venom (HcyV), respectively, and these toxins could be grouped into 15 functional categories, mainly proteinases, phospholipases, three-finger toxins (3FTxs), lectins, protease inhibitors, ion channel inhibitors, cysteine-rich venom proteins (CRVPs) and snake venom metalloproteases (SVMPs). The constituent ratio of each toxin category varied between HcuV and HcyV with 3FTx (54% in HcuV/69% in HcyV) and PLA2 (38% in HcuV/22% in HcyV) unanimously ranked as the top two most abundant families. Both HcuV and HcyV exhibited relatively high lethality (LD50 values in mice of 0.34 μg/g and 0.24 μg/g, respectively), specific PLA2 activity and hemolytic activity. On the basis of several previous reports of HcuV and HcyV collected from other areas, these findings greatly expand our understanding of geographical variation and interspecies diversity of the two sea snake venoms and can provide a scientific basis for the development of specific sea snake antivenom in the future.", "gemini": "Chúng tôi đã mô tả đặc điểm và so sánh các hồ sơ protein nọc độc của Hydrophis curtus (đồng nghĩa: Lapemis hardwickii, Lapemis curtus và Hydrophis hardwickii) và Hydrophis cyanocinctus, hai đại diện của rắn biển có nọc độc quan trọng về mặt y học ở vùng biển Trung Quốc bằng cách sử dụng các phương pháp tiếp cận về protein học. Tổng cộng 47 và 38 độc tố được cho là đã được xác định trong nọc H. curtus (HcuV) và nọc H. cyanocinctus (HcyV), tương ứng, và các độc tố này có thể được nhóm thành 15 loại chức năng, chủ yếu là proteinase, phospholipase, độc tố ba ngón (3FTx), lectin, chất ức chế protease, chất ức chế kênh ion, protein nọc độc giàu cysteine (CRVP) và metalloprotease nọc rắn (SVMP). Tỷ lệ thành phần của mỗi loại độc tố khác nhau giữa HcuV và HcyV với 3FTx (54% trong HcuV/69% trong HcyV) và PLA2 (38% trong HcuV/22% trong HcyV) được xếp hạng nhất trí là hai họ phong phú nhất. Cả HcuV và HcyV đều biểu hiện độc tính tương đối cao (giá trị LD50 ở chuột là 0,34 μg/g và 0,24 μg/g, tương ứng), hoạt tính PLA2 đặc hiệu và hoạt tính tan máu. Trên cơ sở một số báo cáo trước đây về HcuV và HcyV thu thập được từ các khu vực khác, những phát hiện này mở rộng đáng kể sự hiểu biết của chúng ta về sự thay đổi về mặt địa lý và sự đa dạng giữa các loài của hai loại nọc rắn biển và có thể cung cấp cơ sở khoa học cho sự phát triển của huyết thanh kháng nọc rắn biển đặc hiệu trong tương lai."} +{"idx": 1871155, "en": "Influenza A viruses (IAV) are a major cause of respiratory diseases in pigs. Invariant natural killer T (iNKT) cells are an innate-like T cell subset that contribute significantly to IAV resistance in mice. In the current work, we explored whether expanding and activating iNKT cells with the iNKT cell superagonist α-galactosylceramide (α-GalCer) would change the course of an IAV infection in pigs. In one study, α-GalCer was administered to pigs intramuscularly (i.m.) 9 days before infection, which systemically expanded iNKT cells. In another study, α-GalCer was administered intranasally (i.n.) 2 days before virus infection to activate mucosal iNKT cells. Despite a synergistic increase in iNKT cells when α-GalCer i.m. treated pigs were infected with IAV, neither approach reduced disease signs, lung pathology, or virus replication. Our results indicate that prophylactic use of iNKT cell agonists to prevent IAV infection is ineffective in pigs. This is significant because this type of approach has been considered for humans whose iNKT cell levels and IAV infections are more similar to those of pigs than mice.", "gemini": "Virus cúm A (IAV) là nguyên nhân chính gây ra các bệnh về đường hô hấp ở lợn. Tế bào T diệt tự nhiên bất biến (iNKT) là một phân nhóm tế bào T giống như tế bào bẩm sinh góp phần đáng kể vào khả năng kháng IAV ở chuột. Trong nghiên cứu hiện tại, chúng tôi đã khám phá xem việc mở rộng và kích hoạt các tế bào iNKT bằng siêu chất chủ vận tế bào iNKT α-galactosylceramide (α-GalCer) có làm thay đổi quá trình nhiễm IAV ở lợn hay không. Trong một nghiên cứu, α-GalCer đã được tiêm bắp (i.m.) cho lợn 9 ngày trước khi nhiễm trùng, giúp mở rộng toàn thân các tế bào iNKT. Trong một nghiên cứu khác, α-GalCer đã được nhỏ mũi (i.n.) 2 ngày trước khi nhiễm vi-rút để kích hoạt các tế bào iNKT niêm mạc. Mặc dù có sự gia tăng hiệp đồng các tế bào iNKT khi lợn được điều trị α-GalCer i.m. bị nhiễm IAV, nhưng không có cách tiếp cận nào làm giảm các dấu hiệu bệnh, bệnh lý phổi hoặc sự sao chép vi-rút. Kết quả của chúng tôi chỉ ra rằng việc sử dụng các chất chủ vận tế bào iNKT để phòng ngừa nhiễm IAV ở lợn là không hiệu quả. Điều này rất quan trọng vì loại phương pháp tiếp cận này đã được xem xét đối với những người có mức tế bào iNKT và nhiễm IAV giống với lợn hơn là chuột."} +{"idx": 1871156, "en": "Lowering intraocular pressure (IOP) is the most effective treatment of glaucoma, however most of the current available glaucoma drugs target a single molecule. MicroRNAs (miRNAs) are noncoding RNAs that target a network of molecules. This study aims to investigate the role of miR-21-5p in regulating IOP and the mechanism of function. miR-21-5p mimics was topically applied to C57/BL6 mouse eyes, which significantly increased miR-21-5p expression in the conventional outflow tissue and reduced IOP by a maximum of 17.77% at 24 h after treatment. The conventional outflow facility measured by ex vivo moue eye perfusion of miR-21-5p was significantly increased by 60.14%. Moreover, miR-21-5p overexpression significantly reduced the transendothelial electrical resistance in porcine angular aqueous plexus cells. Transcriptome analysis and further quantification by Western blot and PCR revealed that SMAD7 and FGF18 might be the downstream target of miR-21-5p in regulating aqueous humor outflow. The predicted functional pathways PTEN/eNOS, RhoB/pMLC and TIMP3/MMP9 were significantly altered after miR-21-5p transfection. Dual luciferase assay verified the direct targets of miR-21-5p. In conclusion, miR-21-5p seems to regulate IOP by modulating multiple genes that are associated with aqueous humor outflow, including genes those regulating cell adhesion, cytoskeletal dynamics and extracellular matrix turnover. Thus, miR-21-5p represents a new therapeutic strategy for glaucoma and a viable alternative to existing multidrug regimens.", "gemini": "Giảm nhãn áp nội nhãn (IOP) là phương pháp điều trị bệnh tăng nhãn áp hiệu quả nhất, tuy nhiên hầu hết các loại thuốc điều trị bệnh tăng nhãn áp hiện có đều nhắm vào một phân tử duy nhất. MicroRNA (miRNA) là RNA không mã hóa nhắm vào một mạng lưới các phân tử. Nghiên cứu này nhằm mục đích tìm hiểu vai trò của miR-21-5p trong việc điều chỉnh IOP và cơ chế hoạt động. Các chất bắt chước miR-21-5p được nhỏ tại chỗ vào mắt chuột C57/BL6, làm tăng đáng kể biểu hiện miR-21-5p trong mô dòng chảy thông thường và giảm IOP tối đa 17,77% sau 24 giờ điều trị. Cơ sở dòng chảy thông thường được đo bằng phương pháp tưới máu mắt chuột ex vivo của miR-21-5p đã tăng đáng kể 60,14%. Hơn nữa, việc tăng biểu hiện quá mức miR-21-5p làm giảm đáng kể điện trở điện xuyên nội mạc ở các tế bào đám rối góc nước lợn. Phân tích bản sao và định lượng thêm bằng phương pháp Western blot và PCR cho thấy SMAD7 và FGF18 có thể là mục tiêu hạ nguồn của miR-21-5p trong việc điều chỉnh dòng chảy dịch nội nhãn. Các con đường chức năng được dự đoán PTEN/eNOS, RhoB/pMLC và TIMP3/MMP9 đã bị thay đổi đáng kể sau khi chuyển gen miR-21-5p. Thử nghiệm luciferase kép đã xác minh các mục tiêu trực tiếp của miR-21-5p. Tóm lại, miR-21-5p dường như điều chỉnh IOP bằng cách điều chỉnh nhiều gen liên quan đến dòng chảy dịch nội nhãn, bao gồm các gen điều chỉnh độ bám dính của tế bào, động lực học của bộ khung tế bào và sự thay đổi ma trận ngoại bào. Do đó, miR-21-5p đại diện cho một chiến lược điều trị mới cho bệnh tăng nhãn áp và là một lựa chọn thay thế khả thi cho các chế độ đa thuốc hiện có."} +{"idx": 1871157, "en": "When extrapolating data from animal toxicological studies a default factor (dUF) of 100 is applied to derive a heath based guidance value. The UF takes into account the interspecies differences (ID) and the intraspecies variability (IV). When re-evaluating the safety of phosphates used as food additives nephrocalcinosis was identified as the critical endpoint. The underlying mechanism for nephrocalcinosis was attributed to the precipitation of calcium phosphate in the kidney, depending on its solubility, irrespective of the species and the population. Based on the mechanism, the volume of primary urine, for which the glomerular filtration rate (GFR) was used as a proxy, was considered to be the only parameter relevant for ID and IV. Median value of GFR in rats was 4.0 ml/min/kg bw. In humans it was 1.6 ml/min/kg bw in healthy adults and 0.9 in elderly. These values were calculated from the distribution of the GFR data from 8 studies in rats (n = 191), 16 studies in adults (n = 1540) and 5 studies in elderly (n = 2608). Multiplying the distribution of the ratio rat/healthy humans (ID) with the distribution of the ratio healthy humans/elderly human (IV) resulted in a phosphate specific factor of 4.5 (3.3-6.7) (median; 25th - 75th percentile).", "gemini": "Khi ngoại suy dữ liệu từ các nghiên cứu độc tính ở động vật, một hệ số mặc định (dUF) là 100 được áp dụng để có được giá trị hướng dẫn dựa trên sức khỏe. UF có tính đến sự khác biệt giữa các loài (ID) và sự thay đổi trong loài (IV). Khi đánh giá lại tính an toàn của phosphate được sử dụng làm phụ gia thực phẩm, bệnh vôi hóa thận được xác định là điểm cuối quan trọng. Cơ chế cơ bản gây ra bệnh vôi hóa thận được cho là do sự kết tủa của canxi phosphate trong thận, tùy thuộc vào độ hòa tan của nó, không phụ thuộc vào loài và quần thể. Dựa trên cơ chế này, thể tích nước tiểu chính, trong đó tốc độ lọc cầu thận (GFR) được sử dụng làm đại diện, được coi là thông số duy nhất có liên quan đến ID và IV. Giá trị trung bình của GFR ở chuột là 4,0 ml/phút/kg trọng lượng cơ thể. Ở người, giá trị này là 1,6 ml/phút/kg trọng lượng cơ thể ở người lớn khỏe mạnh và 0,9 ở người cao tuổi. Các giá trị này được tính toán từ phân bố dữ liệu GFR từ 8 nghiên cứu trên chuột (n = 191), 16 nghiên cứu trên người lớn (n = 1540) và 5 nghiên cứu trên người cao tuổi (n = 2608). Việc nhân phân bố tỷ lệ chuột/người khỏe mạnh (ID) với phân bố tỷ lệ người khỏe mạnh/người cao tuổi (IV) dẫn đến hệ số riêng cho phosphate là 4,5 (3,3-6,7) (trung vị; phần trăm thứ 25 - 75)."} +{"idx": 1871158, "en": "Affecting over 320 million people around the world, depression has become a formidable challenge for modern medicine. In addition, an increasing number of studies cast doubt on the monoamine theory of depressive disorder and, worryingly, antidepressant medications only significantly benefit patients with severe depression. Thus, it is not surprising that researchers have shown an increased interest in new theories attempting to explain the pathogenesis of this disease. One example is the excitatory/inhibitory transmission imbalance theory. These abnormalities involve glutamate and γ-aminobutyric acid (GABA) signaling. Studies on GABAB receptors and their antagonists are particularly promising for the treatment of depressive disorders. In this paper, intracellular pathways controlled by GABAB receptors and their links to depression are described, including the impact of ketamine on GABAergic synaptic transmission.", "gemini": "Ảnh hưởng đến hơn 320 triệu người trên toàn thế giới, trầm cảm đã trở thành một thách thức to lớn đối với y học hiện đại. Ngoài ra, ngày càng có nhiều nghiên cứu đặt nghi vấn về thuyết monoamine về rối loạn trầm cảm và đáng lo ngại là thuốc chống trầm cảm chỉ có lợi đáng kể cho những bệnh nhân bị trầm cảm nặng. Do đó, không có gì ngạc nhiên khi các nhà nghiên cứu đã thể hiện sự quan tâm ngày càng tăng đối với các lý thuyết mới cố gắng giải thích về quá trình sinh bệnh của căn bệnh này. Một ví dụ là lý thuyết mất cân bằng truyền dẫn kích thích/ức chế. Những bất thường này liên quan đến tín hiệu glutamate và γ-aminobutyric acid (GABA). Các nghiên cứu về thụ thể GABAB và chất đối kháng của chúng đặc biệt hứa hẹn cho việc điều trị các rối loạn trầm cảm. Trong bài báo này, các con đường nội bào do thụ thể GABAB kiểm soát và mối liên quan của chúng với bệnh trầm cảm được mô tả, bao gồm cả tác động của ketamine lên quá trình truyền synap GABAergic."} +{"idx": 1871159, "en": "Accounts regarding the use of novel psychoactive substances continue to escalate annually. These include reports on substituted benzofurans (SBs), such as 1-(1-benzofuran-2-yl)-N-ethylpropan-2-amine (2-EAPB) and 1-(1-benzofuran-5-yl)-N-ethylpropan-2-amine (5-EAPB). Reports on the deaths and adverse consequences from the use of SBs warrant the investigation of their mechanism, possibly predicting the effects of similar compounds. Accordingly, we investigated the possible rewarding and reinforcing effects of 2-EAPB and 5-EAPB through conditioned place preference (CPP), self-administration, and locomotor sensitization tests. We also determined the possible influence of 2-EAPB and 5-EAPB administration on dopamine- and plasticity-related proteins in the nucleus accumbens and ventral tegmental area. 2-EAPB and 5-EAPB induced CPP at different doses and were self-administered by rats. Only 5-EAPB induced locomotor sensitization in mice. 2-EAPB and 5-EAPB did not alter the expressions of dopamine D1 and D2 receptors in the nucleus accumbens, nor changed tyrosine hydroxylase and dopamine transporter expressions in the ventral tegmental area. Both 2-EAPB and 5-EAPB enhanced deltaFosB, but not transcription factor cyclic AMP-response-element binding protein and brain-derived neurotrophic factor in the nucleus accumbens. Hence, the potential rewarding and reinforcing effects on rodents induced by 2-EAPB and 5-EAPB may possibly be associated with alterations in other neurotransmitter systems (besides mesolimbic) and/or neuro-plastic modifications.", "gemini": "Các báo cáo về việc sử dụng các chất hướng thần mới lạ tiếp tục tăng hàng năm. Chúng bao gồm các báo cáo về benzofuran thay thế (SB), chẳng hạn như 1-(1-benzofuran-2-yl)-N-ethylpropan-2-amine (2-EAPB) và 1-(1-benzofuran-5-yl)-N-ethylpropan-2-amine (5-EAPB). Các báo cáo về tử vong và hậu quả bất lợi từ việc sử dụng SB đảm bảo việc điều tra cơ chế của chúng, có khả năng dự đoán tác dụng của các hợp chất tương tự. Theo đó, chúng tôi đã điều tra các tác dụng có thể có lợi và củng cố của 2-EAPB và 5-EAPB thông qua các bài kiểm tra sở thích vị trí có điều kiện (CPP), tự dùng thuốc và nhạy cảm với vận động. Chúng tôi cũng xác định ảnh hưởng có thể có của việc sử dụng 2-EAPB và 5-EAPB đối với các protein liên quan đến dopamine và tính dẻo trong nhân accumbens và vùng tegmental bụng. 2-EAPB và 5-EAPB gây ra CPP ở các liều khác nhau và được chuột tự sử dụng. Chỉ có 5-EAPB gây ra nhạy cảm với vận động ở chuột. 2-EAPB và 5-EAPB không làm thay đổi biểu hiện của thụ thể dopamine D1 và D2 trong nhân accumbens, cũng không làm thay đổi biểu hiện tyrosine hydroxylase và dopamine transporter trong vùng tegmental bụng. Cả 2-EAPB và 5-EAPB đều tăng cường deltaFosB, nhưng không phải yếu tố phiên mã protein liên kết yếu tố đáp ứng vòng AMP và yếu tố thần kinh có nguồn gốc từ não trong nhân accumbens. Do đó, các tác dụng có thể có lợi và củng cố trên động vật gặm nhấm do 2-EAPB và 5-EAPB gây ra có thể liên quan đến những thay đổi trong các hệ thống dẫn truyền thần kinh khác (bên cạnh mesolimbic) và/hoặc những thay đổi về tính dẻo thần kinh."} +{"idx": 1871160, "en": "Takotsubo Syndrome (TS) is a kind of acute cardiac syndrome with a complex pathophysiological mechanism that remains to be elucidated. The relationship between TS and reactive oxygen species has received increasing attention over in recent years. Therefore, the relationship between TS and reactive oxygen species was investigated in vivo and in vitro. Isoprenaline (ISO) was used to induce TS and tempol (quercetin) was selected as a scavenger to eliminate reactive oxygen species in animal experiments, and echocardiography was used to determine the incidence of TS. The H9C2 cells were cultured with different reagents to investigate the detailed mechanism; Reactive oxygen species levels and mitochondrial function were evaluated. Cell apoptosis rate was analyzed by TUNEL staining and the proteins involved in the signaling pathways were examined by Western blotting. It was found that a high dose of tempol almost eliminated TS and protected the cardiac function. Moreover, tempol also decreased the reactive oxygen species levels and reduced lipid droplet deposition in myocardial tissue. In terms of the cultured cells, tempol preconditioning decreased reactive oxygen species production as well as lipid droplet deposition, and protected the mitochondrial function by reducing mitochondrial swelling, thereby maintaining the mitochondrial membrane potential (ΔΨm) at a level that was higher than that of controls. Furthermore, tempol could reduce cells apoptosis after ISO treatment and decrease the protein level of p38, which is a member of the MAPK family, which and thus plays an important role in regulating cells apoptosis. This antiapoptotic effect of tempol was similar to that of a control reagent, SB203580, which is a specific inhibitor of phospha-p38 (p-p38). This study demonstrated, for the first time, a sudden increase in reactive oxygen species and effects of the downstream cascades play core roles in the development of TS.", "gemini": "Hội chứng Takotsubo (TS) là một loại hội chứng tim cấp với cơ chế bệnh sinh phức tạp vẫn chưa được làm sáng tỏ. Mối quan hệ giữa TS và các loại oxy phản ứng đã nhận được sự chú ý ngày càng tăng trong những năm gần đây. Do đó, mối quan hệ giữa TS và các loại oxy phản ứng đã được nghiên cứu in vivo và in vitro. Isoprenaline (ISO) được sử dụng để gây ra TS và tempol (quercetin) được chọn làm chất loại bỏ để loại bỏ các loại oxy phản ứng trong các thí nghiệm trên động vật và siêu âm tim được sử dụng để xác định tỷ lệ mắc TS. Các tế bào H9C2 được nuôi cấy với các thuốc thử khác nhau để nghiên cứu cơ chế chi tiết; Mức độ oxy phản ứng và chức năng ty thể được đánh giá. Tỷ lệ tế bào chết theo chương trình được phân tích bằng nhuộm TUNEL và các protein liên quan đến các con đường truyền tín hiệu được kiểm tra bằng phương pháp Western blotting. Người ta thấy rằng một liều cao tempol gần như loại bỏ TS và bảo vệ chức năng tim. Hơn nữa, tempol cũng làm giảm mức độ oxy phản ứng và giảm sự lắng đọng giọt lipid trong mô cơ tim. Về các tế bào được nuôi cấy, tiền xử lý bằng tempol làm giảm sản xuất oxy phản ứng cũng như sự lắng đọng giọt lipid và bảo vệ chức năng ty thể bằng cách giảm sưng ty thể, do đó duy trì tiềm năng màng ty thể (ΔΨm) ở mức cao hơn mức của đối chứng. Hơn nữa, tempol có thể làm giảm tế bào chết theo chương trình sau khi xử lý bằng ISO và làm giảm mức protein của p38, một thành viên của họ MAPK, do đó đóng vai trò quan trọng trong việc điều hòa tế bào chết theo chương trình. Tác dụng chống apoptosis này của tempol tương tự như tác dụng của thuốc thử đối chứng SB203580, một chất ức chế đặc hiệu của phospha-p38 (p-p38). Nghiên cứu này đã chứng minh, lần đầu tiên, sự gia tăng đột ngột của các loại oxy phản ứng và tác động của các tầng thác hạ lưu đóng vai trò cốt lõi trong sự phát triển của TS."} +{"idx": 1871161, "en": "GLP-1 receptor agonists are used for the treatment of type 2 diabetes but they may reduce appetite and cause nausea and emesis. We investigated if GLP-1 (7-36) amide can modulate glucose homoeostasis, emesis and feeding via an exendin (9-39)-sensitive mechanism in Suncus murinus. The effect of GLP-1 (7-36) amide on glucose homeostasis was examined using an intraperitoneal glucose tolerance test. In conscious fasted animals, food and water consumption and behavior were measured for 1 h following drug administration. c-Fos expression in the brain was measured using immunohistochemistry. GLP-1 (7-36) amide reduced blood glucose levels dose-dependently. Exendin (9-39) did not modify blood glucose levels but suppressed the glucose-lowering effect of GLP-1 (7-36) amide. GLP-1 (7-36) amide inhibited food and water intake, induced emesis and elevated c-Fos expression in the brainstem and hypothalamic nuclei in the brain. Exendin (9-39) antagonised the inhibition of food and water intake and emesis induced by GLP-1 (7-36) amide and the effects on c-Fos expression in the hypothalamus and brainstem, excepting for the bed nucleus of the stria terminalis. These data suggest that the action of GLP-1 (7-36) amide to modulate blood glucose, suppress food and water intake and induce emesis involve GLP-1 receptors in the hypothalamus and brainstem.", "gemini": "Các chất chủ vận thụ thể GLP-1 được sử dụng để điều trị bệnh tiểu đường loại 2 nhưng chúng có thể làm giảm cảm giác thèm ăn và gây buồn nôn và nôn. Chúng tôi đã nghiên cứu xem liệu GLP-1 (7-36) amide có thể điều chỉnh cân bằng glucose, nôn và ăn thông qua cơ chế nhạy cảm với exendin (9-39) ở Suncus murinus hay không. Tác dụng của GLP-1 (7-36) amide đối với cân bằng glucose đã được kiểm tra bằng cách sử dụng xét nghiệm dung nạp glucose trong phúc mạc. Ở những động vật bị nhịn đói có ý thức, lượng thức ăn và nước tiêu thụ và hành vi được đo trong vòng 1 giờ sau khi dùng thuốc. Biểu hiện c-Fos trong não được đo bằng phương pháp miễn dịch mô hóa học. GLP-1 (7-36) amide làm giảm nồng độ glucose trong máu theo liều dùng. Exendin (9-39) không làm thay đổi nồng độ glucose trong máu nhưng ức chế tác dụng hạ glucose của GLP-1 (7-36) amide. GLP-1 (7-36) amide ức chế lượng thức ăn và nước nạp vào, gây nôn và làm tăng biểu hiện c-Fos ở thân não và nhân vùng dưới đồi trong não. Exendin (9-39) đối kháng với sự ức chế lượng thức ăn và nước nạp vào và nôn do GLP-1 (7-36) amide gây ra và các tác dụng đối với biểu hiện c-Fos ở vùng dưới đồi và thân não, ngoại trừ nhân nền của hạt nhân cuối. Dữ liệu này cho thấy rằng tác dụng của GLP-1 (7-36) amide trong việc điều chỉnh glucose máu, ức chế lượng thức ăn và nước nạp vào và gây nôn liên quan đến các thụ thể GLP-1 ở vùng dưới đồi và thân não."} +{"idx": 1871162, "en": "There is considerable concern that naphthenic acids (NA) related to oil extraction can negatively impact reproduction in mammals, yet the mechanisms are unknown. Since placental dysfunction is central to many adverse pregnancy outcomes, the goal of this study was to determine the effects of NA exposure on placental trophoblast cell function. HTR-8/SVneo cells were exposed to a commercial technical NA mixture for 24 hours to assess transcriptional regulation of placentation-related pathways and functional assessment of migration, invasion, and angiogenesis. Pathway analysis suggests that NA treatment resulted in increased epithelial-to-mesenchymal transition. However, there was reduced migration and invasive potential. NA treatment increased angiogenesis-related pathways with a concomitant increase in tube formation. Since decreased trophoblast invasion/migration and aberrant angiogenesis have been associated with placental dysfunction, these findings suggest that it is biologically plausible that exposure to NA may result in altered placental development and/or function.", "gemini": "Có mối quan ngại đáng kể rằng các axit naphthenic (NA) liên quan đến việc khai thác dầu có thể ảnh hưởng tiêu cực đến khả năng sinh sản ở động vật có vú, nhưng cơ chế vẫn chưa được biết rõ. Vì rối loạn chức năng nhau thai là nguyên nhân chính dẫn đến nhiều kết cục thai nghén bất lợi, mục tiêu của nghiên cứu này là xác định tác động của phơi nhiễm NA đối với chức năng tế bào nuôi dưỡng nhau thai. Các tế bào HTR-8/SVneo đã tiếp xúc với hỗn hợp NA kỹ thuật thương mại trong 24 giờ để đánh giá sự điều hòa phiên mã của các con đường liên quan đến nhau thai và đánh giá chức năng của quá trình di chuyển, xâm lấn và hình thành mạch máu. Phân tích con đường cho thấy rằng điều trị bằng NA dẫn đến quá trình chuyển đổi biểu mô thành trung mô tăng lên. Tuy nhiên, có sự giảm khả năng di chuyển và xâm lấn. Điều trị bằng NA làm tăng các con đường liên quan đến hình thành mạch máu với sự gia tăng đồng thời trong quá trình hình thành ống. Vì sự xâm lấn/di chuyển của tế bào nuôi dưỡng nhau thai giảm và sự hình thành mạch máu bất thường có liên quan đến rối loạn chức năng nhau thai, những phát hiện này cho thấy rằng về mặt sinh học, có khả năng tiếp xúc với NA có thể dẫn đến sự phát triển và/hoặc chức năng nhau thai bị thay đổi."} +{"idx": 1871163, "en": "Ischemic stroke represents a serious medical condition which could cause survivors suffer from long-term and even lifetime disabilities. After a stroke attack, the brain would undergo varying degrees of recovery, in which the central nervous system could be reorganized spontaneously or with the help of appropriate rehabilitation. Magnetic resonance imaging (MRI) is a non-invasive technique which can provide comprehensive information on structural, functional and metabolic features of brain tissue. In the last decade, there has been an increased technical advancement in MR techniques such as voxel-based morphological analysis (VBM), diffusion magnetic resonance imaging (dMRI), functional magnetic resonance imaging (fMRI), arterial spin-labeled perfusion imaging (ASL), magnetic sensitivity weighted imaging (SWI), quantitative sensitivity magnetization (QSM) and magnetic resonance spectroscopy (MRS) which have been proven to be a valuable tool to study the brain tissue reorganization. Due to MRI indices of neuroplasticity related to neurological outcome could be translated to the clinic. The ultimate goal of this review is to equip readers with a fundamental understanding of advanced MR techniques and their corresponding clinical application for improving the ability to predict neuroplasticity that are most suitable for stroke management.", "gemini": "Đột quỵ do thiếu máu cục bộ là một tình trạng bệnh lý nghiêm trọng có thể khiến những người sống sót phải chịu các khuyết tật dài hạn và thậm chí là suốt đời. Sau một cơn đột quỵ, não sẽ trải qua các mức độ phục hồi khác nhau, trong đó hệ thần kinh trung ương có thể được tổ chức lại một cách tự phát hoặc với sự trợ giúp của quá trình phục hồi chức năng thích hợp. Chụp cộng hưởng từ (MRI) là một kỹ thuật không xâm lấn có thể cung cấp thông tin toàn diện về các đặc điểm về cấu trúc, chức năng và chuyển hóa của mô não. Trong thập kỷ qua, đã có sự tiến bộ vượt bậc về mặt kỹ thuật trong các kỹ thuật MR như phân tích hình thái dựa trên voxel (VBM), chụp cộng hưởng từ khuếch tán (dMRI), chụp cộng hưởng từ chức năng (fMRI), chụp tưới máu động mạch đánh dấu spin (ASL), chụp ảnh trọng số độ nhạy từ (SWI), định lượng từ hóa độ nhạy (QSM) và phổ cộng hưởng từ (MRS) đã được chứng minh là một công cụ có giá trị để nghiên cứu sự tổ chức lại của mô não. Do các chỉ số MRI về tính dẻo thần kinh liên quan đến kết cục thần kinh có thể được chuyển thành lâm sàng. Mục tiêu cuối cùng của bài đánh giá này là trang bị cho người đọc sự hiểu biết cơ bản về các kỹ thuật MR tiên tiến và ứng dụng lâm sàng tương ứng của chúng để cải thiện khả năng dự đoán tính dẻo thần kinh phù hợp nhất cho việc quản lý đột quỵ."} +{"idx": 1871164, "en": "BACKGROUND Non-small cell lung cancer (NSCLC) is the most commonly diagnosed solid cancer and the main origin of cancer-related deaths worldwide. Current strategies to treat advanced NSCLC are based on a combined approach of targeted therapy and chemotherapy. But most patients will eventually get resistance to either chemotherapy or targeted therapy, leading to the poor prognosis. The mechanism of NSCLC drug resistance is inconclusive and is affected by multiple factors. Long non-coding RNAs (LncRNAs) are non-coding RNAs (ncRNAs) longer than 200 nucleotides. Recent studies show that lncRNAs are involved in many cellular physiological activities, including drug resistance of NSCLC. It is of great clinical significance to understand the specific mechanisms and the role of lncRNAs in it. CONCLUSIONS Herein, we focus on the functional roles and the underlying mechanisms of lncRNAs in acquired drug resistance of NSCLC. LncRNAs have potential values as novel prognostic biomarkers and even therapeutic targets in the clinical management of NSCLC.", "gemini": "BỐI CẢNH Ung thư phổi không phải tế bào nhỏ (NSCLC) là loại ung thư rắn được chẩn đoán phổ biến nhất và là nguyên nhân chính gây tử vong liên quan đến ung thư trên toàn thế giới. Các chiến lược hiện tại để điều trị NSCLC tiến triển dựa trên phương pháp kết hợp giữa liệu pháp nhắm trúng đích và hóa trị. Nhưng hầu hết bệnh nhân cuối cùng sẽ bị kháng thuốc hóa trị hoặc liệu pháp nhắm trúng đích, dẫn đến tiên lượng kém. Cơ chế kháng thuốc NSCLC vẫn chưa có kết luận và bị ảnh hưởng bởi nhiều yếu tố. RNA không mã hóa dài (LncRNA) là RNA không mã hóa (ncRNA) dài hơn 200 nucleotide. Các nghiên cứu gần đây cho thấy lncRNA tham gia vào nhiều hoạt động sinh lý của tế bào, bao gồm cả tình trạng kháng thuốc của NSCLC. Việc hiểu được các cơ chế cụ thể và vai trò của lncRNA trong đó có ý nghĩa lâm sàng rất lớn. KẾT LUẬN Trong bài viết này, chúng tôi tập trung vào các vai trò chức năng và các cơ chế tiềm ẩn của lncRNA trong tình trạng kháng thuốc mắc phải của NSCLC. LncRNA có giá trị tiềm năng như các dấu ấn sinh học tiên lượng mới và thậm chí là các mục tiêu điều trị trong quản lý lâm sàng NSCLC."} +{"idx": 1871165, "en": "AIMS This study aims to cast light on immunocytometric alterations in COVID-19, a potentially fatal viral infection with heterogeneous clinical expression and a not completely defined pathophysiology. METHODS We studied 35 COVID patients at hospital admission testing by cytofluorimetry a large panel of lymphocyte subpopulations and serum tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17A and the soluble receptor of IL-17A (IL-17RA). KEY FINDINGS At hospital admission, total lymphocytes and most T and B subpopulations were reduced in 50-80% of patients, with close relationship to disease severity. While activated T helper 1 (TH1) and TH17 cells resulted normal or higher. Serum IL-6 was increased in all patients, while TNF-α and IL-17A were higher in advanced stages. A patient subset with low severity had very high IL-17RA levels. Tocilizumab treatment caused an increase of IL-17A in 3/6 patients and a reduction in 3 others, while the lymphocyte number increased in 3 patients and did not change in the others. SIGNIFICANCE Cytofluorimetry revealed a functional exhaustion of most lymphocyte populations in COVID patients not involving activated TH1 and TH17. Consequently, there was a relevant cytokines production that contributes to impair the respiratory inflammation. The increase of TH17 and IL-17 in a subset of cases and the evidence of a significant increase of IL-17RA (that prevents the interaction of IL-17 with the cell receptor) in patients with low severity suggest that some patients could benefit from monoclonal antibodies treatment targeting IL-17 pathway. Immunocytofluorimetric markers may contribute to a personalized therapy in COVID patients.", "gemini": "MỤC ĐÍCH Nghiên cứu này nhằm làm sáng tỏ những thay đổi về miễn dịch tế bào trong COVID-19, một bệnh nhiễm trùng do vi-rút có khả năng gây tử vong với biểu hiện lâm sàng không đồng nhất và bệnh sinh chưa được xác định hoàn toàn. PHƯƠNG PHÁP Chúng tôi đã nghiên cứu 35 bệnh nhân COVID khi nhập viện bằng cách xét nghiệm tế bào bằng phương pháp đo lưu lượng tế bào một nhóm lớn các phân nhóm tế bào lympho và yếu tố hoại tử khối u trong huyết thanh (TNF)-α, interleukin (IL)-6, IL-17A và thụ thể hòa tan của IL-17A (IL-17RA). NHỮNG PHÁT HIỆN CHÍNH Khi nhập viện, tổng số tế bào lympho và hầu hết các phân nhóm T và B đã giảm ở 50-80% bệnh nhân, có mối quan hệ chặt chẽ với mức độ nghiêm trọng của bệnh. Trong khi tế bào lympho T hỗ trợ 1 (TH1) và TH17 hoạt hóa có kết quả bình thường hoặc cao hơn. IL-6 trong huyết thanh tăng ở tất cả bệnh nhân, trong khi TNF-α và IL-17A cao hơn ở giai đoạn tiến triển. Một nhóm bệnh nhân có mức độ nghiêm trọng thấp có mức IL-17RA rất cao. Điều trị Tocilizumab làm tăng IL-17A ở 3/6 bệnh nhân và giảm ở 3 bệnh nhân khác, trong khi số lượng tế bào lympho tăng ở 3 bệnh nhân và không thay đổi ở những bệnh nhân khác. Ý NGHĨA Đo lưu lượng tế bào cho thấy sự suy kiệt về chức năng của hầu hết các quần thể tế bào lympho ở bệnh nhân COVID không liên quan đến TH1 và TH17 hoạt hóa. Do đó, có sự sản xuất cytokine có liên quan góp phần làm suy yếu tình trạng viêm đường hô hấp. Sự gia tăng của TH17 và IL-17 trong một nhóm các trường hợp và bằng chứng về sự gia tăng đáng kể của IL-17RA (ngăn tương tác của IL-17 với thụ thể tế bào) ở những bệnh nhân có mức độ nghiêm trọng thấp cho thấy một số bệnh nhân có thể được hưởng lợi từ điều trị bằng kháng thể đơn dòng nhắm vào con đường IL-17. Các dấu ấn miễn dịch tế bào có thể góp phần vào liệu pháp được cá nhân hóa ở bệnh nhân COVID."} +{"idx": 1871166, "en": "Nature relies on complexes of colocated enzymes to efficiently perform multiple catalytic steps. Such enzyme colocalisation promotes substrate channelling, enhances the activity of multiple synergistically acting enzymes and avoids the loss of potentially toxic intermediates. The industrial biotechnology field develops sophisticated methods to mimic natural colocalisation mechanisms to produce increasingly complex bio-based chemicals. Synthetic protein scaffolds are an advanced way to achieve colocalisation of multiple enzymes in one protein complex. The backbone scaffold is composed of multiple domains that are either separated by linkers or fused to self-assembling proteins. Enzymes are recruited to this scaffold by fusing them to domains that bind to orthogonal domains in the scaffold. A particular feature of synthetic protein scaffolds is the control over spatial organisation and enzyme stoichiometry. Several successful examples of synthetic protein scaffolds have been reported, yet the optimisation of such multi-enzyme complexes is a multiparametric, and therefore often empirical process. This review focusses on pioneering scaffolding examples and elaborates on each parameter influencing the activity of these multi-enzyme complexes. Advances in this field are expected to result in a growing catalogue of chemicals that can be produced starting from cheap and widely available renewable materials.", "gemini": "Thiên nhiên dựa vào các phức hợp enzyme đồng vị trí để thực hiện hiệu quả nhiều bước xúc tác. Sự đồng vị trí của enzyme như vậy thúc đẩy kênh dẫn chất, tăng cường hoạt động của nhiều enzyme hoạt động hiệp đồng và tránh mất các chất trung gian có khả năng gây độc. Lĩnh vực công nghệ sinh học công nghiệp phát triển các phương pháp tinh vi để bắt chước các cơ chế đồng vị trí tự nhiên nhằm sản xuất ra các hóa chất có nguồn gốc sinh học ngày càng phức tạp. Giàn protein tổng hợp là một cách tiên tiến để đạt được sự đồng vị trí của nhiều enzyme trong một phức hợp protein. Giàn xương sống bao gồm nhiều miền được phân tách bằng các liên kết hoặc hợp nhất với các protein tự lắp ráp. Enzyme được tuyển dụng vào giàn này bằng cách hợp nhất chúng với các miền liên kết với các miền trực giao trong giàn. Một đặc điểm đặc biệt của giàn protein tổng hợp là khả năng kiểm soát tổ chức không gian và tỷ lượng enzyme. Một số ví dụ thành công về giàn protein tổng hợp đã được báo cáo, tuy nhiên việc tối ưu hóa các phức hợp đa enzyme như vậy là một quá trình đa tham số và do đó thường là quá trình theo kinh nghiệm. Bài đánh giá này tập trung vào các ví dụ đi tiên phong về giàn giáo và trình bày chi tiết về từng thông số ảnh hưởng đến hoạt động của các phức hợp đa enzyme này. Những tiến bộ trong lĩnh vực này dự kiến sẽ dẫn đến danh mục các hóa chất ngày càng tăng có thể được sản xuất bắt đầu từ các vật liệu tái tạo giá rẻ và có sẵn rộng rãi."} +{"idx": 1871167, "en": "History of metabolism originates with yeast making bread. In fact, study based on \"Yeast\" was so crucial in the development of the field of biochemistry that the word \"enzyme\" is derived from the Greek word meaning leavened (yeast). Yeast has always been a point of interest as a eukaryotic model system to demonstrate the metabolites and their function. In recent times their metabolites are widely studied to predict their role in various pathways. Many traditional and analytical techniques have been employed, but its study through metabolomics is of recent interest in research. The present review focuses on details about yeast metabolomics based on preliminary research on various analytical techniques along with computational approaches. The review also aimed to highlight machine learning and various inceptions of yeast metabolomics.", "gemini": "Lịch sử của quá trình trao đổi chất bắt đầu từ việc làm bánh men. Trên thực tế, nghiên cứu dựa trên \"Men\" rất quan trọng trong sự phát triển của lĩnh vực sinh hóa học đến nỗi từ \"enzyme\" có nguồn gốc từ tiếng Hy Lạp có nghĩa là men nở (men). Men luôn là điểm quan tâm như một hệ thống mô hình nhân chuẩn để chứng minh các chất chuyển hóa và chức năng của chúng. Trong thời gian gần đây, các chất chuyển hóa của chúng được nghiên cứu rộng rãi để dự đoán vai trò của chúng trong các con đường khác nhau. Nhiều kỹ thuật phân tích và truyền thống đã được sử dụng, nhưng nghiên cứu của nó thông qua nghiên cứu chuyển hóa là mối quan tâm gần đây trong nghiên cứu. Đánh giá hiện tại tập trung vào các chi tiết về chuyển hóa nấm men dựa trên nghiên cứu sơ bộ về các kỹ thuật phân tích khác nhau cùng với các phương pháp tính toán. Đánh giá cũng nhằm mục đích làm nổi bật máy học và các khởi đầu khác nhau của chuyển hóa nấm men."} +{"idx": 1871168, "en": "BACKGROUND QTc interval (QTc) prolongation is seen on the post-arrest electrocardiogram (ECG) of many out of hospital cardiac arrest (OHCA) survivors. It remains unclear whether this is a transient phenomenon or a manifestation of an underlying arrhythmic substrate. This observational study assessed the trend of QTc in an unselected group of patients presenting with OHCA. We sought to identify any relationship between QTc, gender and aetiology of arrest. We observed whether targeted temperature management (TTM) is associated with malignant arrhythmia. METHOD We analysed 60 patients presenting with OHCA to the Bristol Heart Institute during a 20-month period. We measured QTc on admission and assessed for persistence, development and resolution of prolongation at up to 5 time points post-OHCA. Aetiology of arrest was divided into coronary, non-coronary or primary arrhythmic to investigate for patterns in QTc behaviour. RESULTS 81.7% (49/60) of arrests were attributed to an acute coronary event. 55% (33/60) had QTc prolongation on admission, of which 79% resolved. There were no significant differences in QTc behaviour by aetiology. One patient presenting with a normal QTc, developed prolongation during admission and received a genetic diagnosis of Long QT Syndrome. TTM was employed in 57/60, with no increased incidence of malignant arrhythmia. CONCLUSIONS Prolonged QTc on admission does not imply a primary arrhythmic aetiology and resolves in the majority pre-discharge. However, an initial normal QTc post-OHCA does not preclude a diagnosis of Long QT syndrome, highlighting the importance of thorough investigations in these patients. TTM appears safe from a cardiac perspective.", "gemini": "BỐI CẢNH Khoảng QTc kéo dài được thấy trên điện tâm đồ sau khi bắt giữ (ECG) của nhiều người sống sót sau khi ngừng tim ngoài bệnh viện (OHCA). Vẫn chưa rõ liệu đây có phải là một hiện tượng thoáng qua hay là biểu hiện của một cơ chất loạn nhịp tiềm ẩn. Nghiên cứu quan sát này đánh giá xu hướng của QTc trong một nhóm bệnh nhân không được lựa chọn có biểu hiện OHCA. Chúng tôi tìm cách xác định bất kỳ mối quan hệ nào giữa QTc, giới tính và nguyên nhân gây ra tình trạng ngừng tim. Chúng tôi quan sát xem liệu quản lý nhiệt độ mục tiêu (TTM) có liên quan đến loạn nhịp ác tính hay không. PHƯƠNG PHÁP Chúng tôi đã phân tích 60 bệnh nhân có biểu hiện OHCA tại Viện Tim Bristol trong giai đoạn 20 tháng. Chúng tôi đã đo QTc khi nhập viện và đánh giá sự dai dẳng, phát triển và giải quyết tình trạng kéo dài tại tối đa 5 thời điểm sau OHCA. Nguyên nhân gây ra tình trạng ngừng tim được chia thành động mạch vành, không phải động mạch vành hoặc loạn nhịp chính để điều tra các mô hình trong hành vi QTc. KẾT QUẢ 81,7% (49/60) các trường hợp ngừng tim là do một biến cố động mạch vành cấp tính. 55% (33/60) có kéo dài QTc khi nhập viện, trong đó 79% đã giải quyết. Không có sự khác biệt đáng kể về hành vi QTc theo nguyên nhân. Một bệnh nhân có QTc bình thường, kéo dài trong quá trình nhập viện và được chẩn đoán di truyền là Hội chứng QT dài. TTM được sử dụng ở 57/60, không có trường hợp loạn nhịp ác tính nào tăng lên. KẾT LUẬN QTc kéo dài khi nhập viện không ngụ ý nguyên nhân loạn nhịp chính và giải quyết ở phần lớn trước khi xuất viện. Tuy nhiên, QTc bình thường ban đầu sau OHCA không loại trừ chẩn đoán Hội chứng QT dài, làm nổi bật tầm quan trọng của các cuộc điều tra kỹ lưỡng ở những bệnh nhân này. TTM có vẻ an toàn theo quan điểm tim mạch."} +{"idx": 1871169, "en": "Wilson disease (WD) is an autosomal-recessive disorder associated with the impaired copper metabolism, resulting in hepatic and neurologic manifestations. D-Pencillamine (DPA) is a first-line of treatment however, monoisoamyl 2, 3-dimercaptosuccinic acid (MiADMSA), is gaining recognition recently as a future chelating agent of choice. We evaluated the effects of MiADMSA against copper-induced (20 mg/kg, orally, once, daily for 16 weeks) hepatic and immunological changes in the male Sprague Dawley (SD) rats. Copper overload increased the levels of pro-oxidant and concurrently decreased the levels of antioxidant enzymes in the liver. Increased oxidative stress triggered the up-regulation of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) in the liver and down-regulated the anti-inflammatory cytokine IL-4. Altered liver function parameters as well as serum immunoglobulins' (IgG, IgA, IgE, and IgM) levels, were also noted. MiADMSA treatment restored most of copper altered biochemical and immunological changes. Further, the histopathological changes proved that MiADMSA treatment ameliorated copper induced hepatic injury. Infra red spectra of liver tissue indicated shift in the characteristic -OH peak during copper exposure while the shifting came to normal in MiADMSA administered rat liver. We conclude that MiADMSA could be a promising antidote for the chronic copper toxicity and possibly in the clinical management of WD.", "gemini": "Bệnh Wilson (WD) là một rối loạn lặn trên nhiễm sắc thể thường liên quan đến rối loạn chuyển hóa đồng, dẫn đến các biểu hiện về gan và thần kinh. D-Pencillamine (DPA) là phương pháp điều trị đầu tay, tuy nhiên, axit monoisoamyl 2, 3-dimercaptosuccinic (MiADMSA) gần đây được công nhận là chất tạo phức trong tương lai được lựa chọn. Chúng tôi đã đánh giá tác dụng của MiADMSA đối với các thay đổi về miễn dịch và gan do đồng gây ra (20 mg / kg, uống, một lần, hàng ngày trong 16 tuần) ở chuột Sprague Dawley (SD) đực. Quá tải đồng làm tăng mức độ pro-oxidant và đồng thời làm giảm mức độ của các enzym chống oxy hóa trong gan. Căng thẳng oxy hóa gia tăng kích hoạt sự điều hòa tăng các cytokine tiền viêm (TNF-α, IL-1β, IL-6) trong gan và điều hòa giảm cytokine chống viêm IL-4. Các thông số chức năng gan bị thay đổi cũng như nồng độ miễn dịch huyết thanh (IgG, IgA, IgE và IgM). Điều trị bằng MiADMSA đã phục hồi hầu hết các thay đổi về sinh hóa và miễn dịch do đồng thay đổi. Hơn nữa, các thay đổi về mô bệnh học chứng minh rằng điều trị bằng MiADMSA làm giảm tổn thương gan do đồng gây ra. Phổ hồng ngoại của mô gan cho thấy sự thay đổi ở đỉnh -OH đặc trưng trong quá trình tiếp xúc với đồng trong khi sự dịch chuyển trở lại bình thường ở gan của chuột được dùng MiADMSA. Chúng tôi kết luận rằng MiADMSA có thể là một chất giải độc đầy hứa hẹn đối với tình trạng ngộ độc đồng mạn tính và có thể trong quản lý lâm sàng WD."} +{"idx": 1871170, "en": "Activation of the Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor E2 related factor 2 (Nrf2) system plays a role in repression of xenobiotic toxicity. The Coriandrum sativum L. leaf extract (CSLE) contains various aliphatic electrophiles such as (E)-2-decenal and (E)-2-dodecenal. In the present study, we examined the activation of Nrf2 coupled to chemical modification of Keap1 mediated by (E)-2-alkenals in CSLE, and the protective role of CSLE and (E)-2-alkenals against inorganic arsenite (iAsIII) cytotoxicity. Ultra-performance liquid chromatography-elevated collision energy mass spectrometry analysis revealed that (E)-2-decenal modified recombinant Keap1 at Cys241, Cys249, Cys257 and His274. Exposure of HepG2 cells to CSLE, (E)-2-decenal, or (E)-2-dodecenal upregulated Nrf2-related downstream signaling such as expression of phase-II xenobiotic-metabolizing enzymes and phase-III transporters involved in cytoprotection against iAsIII. Pretreatment with CSLE or (E)-2-butenal, a prototype of (E)-2-alkenal, prior to iAsIII exposure suppressed accumulation of iAsIII significantly and reduced iAsIII-induced cytotoxicity in cells. Oral administration of CSLE to C57BL/6 mice upregulated downstream proteins of Nrf2 and reduced accumulation of arsenic in liver tissue. The present study indicates that CSLE containing (E)-2-alkenals activates Nrf2, leading to a reduction in arsenic accumulation in vivo.", "gemini": "Việc kích hoạt hệ thống protein 1 liên kết với ECH giống Kelch (Keap1)/nhân tố hạt nhân E2 liên quan đến nhân tố 2 (Nrf2) đóng vai trò trong việc ức chế độc tính của chất lạ. Chiết xuất lá Coriandrum sativum L. (CSLE) chứa nhiều chất ái điện mạch hở như (E)-2-decenal và (E)-2-dodecenal. Trong nghiên cứu này, chúng tôi đã kiểm tra sự kích hoạt của Nrf2 kết hợp với sửa đổi hóa học của Keap1 do (E)-2-alkenal trong CSLE và vai trò bảo vệ của CSLE và (E)-2-alkenal chống lại độc tính tế bào của asenit vô cơ (iAsIII). Phân tích phổ khối năng lượng va chạm nâng cao sắc ký lỏng hiệu suất cực cao cho thấy (E)-2-decenal đã sửa đổi Keap1 tái tổ hợp tại Cys241, Cys249, Cys257 và His274. Việc phơi nhiễm tế bào HepG2 với CSLE, (E)-2-decenal hoặc (E)-2-dodecenal đã điều hòa tín hiệu hạ lưu liên quan đến Nrf2 như biểu hiện của các enzym chuyển hóa chất lạ pha II và các chất vận chuyển pha III liên quan đến bảo vệ tế bào chống lại iAsIII. Xử lý trước bằng CSLE hoặc (E)-2-butenal, một nguyên mẫu của (E)-2-alkenal, trước khi tiếp xúc với iAsIII đã ức chế đáng kể sự tích tụ của iAsIII và giảm độc tính tế bào do iAsIII gây ra trong tế bào. Việc uống CSLE cho chuột C57BL/6 đã điều hòa các protein hạ lưu của Nrf2 và giảm sự tích tụ asen trong mô gan. Nghiên cứu này chỉ ra rằng CSLE chứa (E)-2-alkenal kích hoạt Nrf2, dẫn đến giảm sự tích tụ asen trong cơ thể sống."} +{"idx": 1871171, "en": "The complex pathophysiological mechanisms behind destructive chronic conditions, including cancer, neurodegenerative diseases, diabetes mellitus, cardiovascular diseases, and hepatic failure urge the need for finding related pivotal dysregulated signaling mediators, as well as multi-target therapeutic agents. In the current study, critical roles of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway, as potential therapeutic targets in the pathogenesis of various diseases has been described. This pathway is also interconnected with several downstream inflammatory, oxidative stress, and apoptotic mediators, as dysregulated pathways in chronic diseases. Therefore, identifying novel multi-target agents to attenuate PI3K/Akt, thereby related downstream pathways, is of great importance. Astaxanthin (AST) is a multi-target lipid-soluble keto-carotenoid derived from the varieties of marine organisms, with potential anti-inflammatory, antioxidant and antiapoptotic properties through PI3K/Akt pathway. Nowadays, due to its high nutritional and medicinal value, research on AST is increasing. This review aimed to address PI3K/Akt targeted by AST in several diseases toward clinical applications.", "gemini": "Các cơ chế bệnh sinh phức tạp đằng sau các tình trạng mạn tính phá hủy, bao gồm ung thư, bệnh thoái hóa thần kinh, đái tháo đường, bệnh tim mạch và suy gan thúc đẩy nhu cầu tìm ra các chất trung gian truyền tín hiệu rối loạn liên quan, cũng như các tác nhân điều trị đa đích. Trong nghiên cứu hiện tại, vai trò quan trọng của con đường phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt), là các mục tiêu điều trị tiềm năng trong quá trình sinh bệnh của nhiều bệnh đã được mô tả. Con đường này cũng được kết nối với một số chất trung gian gây viêm, stress oxy hóa và chết theo chương trình hạ lưu, là các con đường rối loạn trong các bệnh mạn tính. Do đó, việc xác định các tác nhân đa đích mới để làm giảm PI3K/Akt, do đó các con đường hạ lưu liên quan, có tầm quan trọng rất lớn. Astaxanthin (AST) là một keto-carotenoid hòa tan trong lipid đa đích có nguồn gốc từ các loại sinh vật biển, với các đặc tính chống viêm, chống oxy hóa và chống apoptosis tiềm tàng thông qua con đường PI3K/Akt. Ngày nay, do giá trị dinh dưỡng và dược tính cao của nó, nghiên cứu về AST ngày càng tăng. Mục đích của bài tổng quan này là giải quyết PI3K/Akt được nhắm mục tiêu bởi AST trong một số bệnh hướng tới các ứng dụng lâm sàng."} +{"idx": 1871172, "en": "Challenges of ophthalmic drug delivery arise not only from the limited solubility of hydrophobic therapeutics, but also the restricted permeability and fast clearance of drugs due to the complex anatomy and physiology of eyes. Excellent biocompatibility of a thermosensitive polymer, PLGA-PEG-PLGA (1800-1500-1800, LA:GA ratio = 3:1), as an ophthalmic delivery system was demonstrated in our previous work. In this study, delivery of dexamethasone using this thermogel via a single subconjunctival injection for prolonged treatment was evaluated with corneal neovascularization using an alkali-burn diseased model in rat. Solubility of dexamethasone in the polymeric solution was increased by 5.2-fold and the resulting drug-loaded solution formed in situ rigid gel at body temperature. Prolonged in vitro release of dexamethasone from the gel structure was noted. Dexamethasone gel formulation was demonstrated to be more effective in reducing the burn stimulus and neovascularization in the rat diseased model. The findings suggest the PLGA-PEG-PLGA in situ gelling system can be applied for ophthalmic drug delivery to achieve sustained drug release and improved efficacy.", "gemini": "Những thách thức của việc đưa thuốc vào mắt không chỉ phát sinh từ độ hòa tan hạn chế của các chất điều trị kỵ nước mà còn do tính thấm hạn chế và độ thanh thải nhanh của thuốc do cấu trúc giải phẫu và sinh lý phức tạp của mắt. Khả năng tương thích sinh học tuyệt vời của một loại polyme nhạy nhiệt, PLGA-PEG-PLGA (1800-1500-1800, tỷ lệ LA:GA = 3:1), như một hệ thống đưa thuốc vào mắt đã được chứng minh trong công trình trước đây của chúng tôi. Trong nghiên cứu này, việc đưa dexamethasone bằng nhiệt gel này thông qua một lần tiêm dưới kết mạc để điều trị kéo dài đã được đánh giá bằng quá trình tân mạch hóa giác mạc sử dụng mô hình bệnh do bỏng kiềm ở chuột. Độ hòa tan của dexamethasone trong dung dịch polyme đã tăng lên 5,2 lần và dung dịch chứa thuốc tạo thành gel cứng tại chỗ ở nhiệt độ cơ thể. Sự giải phóng dexamethasone kéo dài trong ống nghiệm từ cấu trúc gel đã được ghi nhận. Công thức gel dexamethasone đã được chứng minh là hiệu quả hơn trong việc giảm kích thích do bỏng và tân mạch hóa trong mô hình bệnh ở chuột. Những phát hiện này cho thấy hệ thống tạo gel tại chỗ PLGA-PEG-PLGA có thể được áp dụng để đưa thuốc vào mắt để đạt được giải phóng thuốc kéo dài và cải thiện hiệu quả."} +{"idx": 1871173, "en": "The research presented here shows QbD implementation for the optimisation of the key process parameters in electrohydrodynamic atomisation (EHDA). Here, the electrosprayed nanoparticles and electrospun fibers consisting of a polymeric matrix and dye. Eight formulations were assessed consisting of 5% w/v of polycaprolactone (PCL) in dichloromethane (DCM) and 5% w/v polyvinylpyrrolidone (PVP) in ethanol. A full factorial DOE was used to assess the various parameters (applied voltage, deposition distance, flow rate). Further particle and fiber analysis using Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), Thermogravimetric Analysis (TGA), Fourier Transform Infrared Spectroscopy (FTIR), particle/fiber size distribution. In addition to this in vitro release studied were carried out using fluorescein and Rhodamine B as model dyes and in vitro permeation studies were applied. The results show a significant difference in the morphology of resultant structures as well as a more rapid release profile for the PVP particles and fibers in comparison to the sustained release profiles found with PCL. In vitro drug release studies showed 100% drug release after 7 days for PCL particles and showed 100% drug release within 120 min for PVP particles. The release kinetics and the permeation study showed that the MN successfully pierced the membrane and the electrospun MN coating released a large amount of the loaded drug within 6 h. This study has demonstrated the capability of these robust MNs to encapsulate a diverse range drugs within a polymeric matrix giving rise to the potential of developed personalised medical devices.", "gemini": "Nghiên cứu được trình bày ở đây cho thấy việc triển khai QbD để tối ưu hóa các thông số quy trình chính trong quá trình phun điện tích hạt (EHDA). Ở đây, các hạt nano điện phun và sợi điện kéo bao gồm một ma trận polyme và thuốc nhuộm. Tám công thức đã được đánh giá bao gồm 5% w/v polycaprolactone (PCL) trong dichloromethane (DCM) và 5% w/v polyvinylpyrrolidone (PVP) trong ethanol. Một DOE thừa số đầy đủ đã được sử dụng để đánh giá các thông số khác nhau (điện áp áp dụng, khoảng cách lắng đọng, tốc độ dòng chảy). Phân tích hạt và sợi sâu hơn bằng Kính hiển vi điện tử quét (SEM), Lượng nhiệt vi sai (DSC), Phân tích nhiệt trọng lượng (TGA), Phổ hồng ngoại biến đổi Fourier (FTIR), phân bố kích thước hạt/sợi. Ngoài ra, các nghiên cứu giải phóng trong ống nghiệm đã được thực hiện bằng cách sử dụng fluorescein và Rhodamine B làm thuốc nhuộm mô hình và các nghiên cứu thẩm thấu trong ống nghiệm đã được áp dụng. Kết quả cho thấy sự khác biệt đáng kể về hình thái của các cấu trúc kết quả cũng như cấu hình giải phóng nhanh hơn đối với các hạt và sợi PVP so với cấu hình giải phóng kéo dài được tìm thấy ở PCL. Các nghiên cứu giải phóng thuốc trong ống nghiệm cho thấy giải phóng thuốc 100% sau 7 ngày đối với các hạt PCL và giải phóng thuốc 100% trong vòng 120 phút đối với các hạt PVP. Động học giải phóng và nghiên cứu thẩm thấu cho thấy MN đã xuyên thủng màng thành công và lớp phủ MN điện kéo giải phóng một lượng lớn thuốc đã nạp trong vòng 6 giờ. Nghiên cứu này đã chứng minh khả năng của các MN mạnh mẽ này trong việc đóng gói nhiều loại thuốc khác nhau trong một ma trận polyme, tạo ra tiềm năng phát triển các thiết bị y tế được cá nhân hóa."} +{"idx": 1871174, "en": "More than 10 years after the Paediatric Regulation came into place there is still a strong need for paediatric medicines for off-patent drug substances. Numerous compounds for which a paediatric formulation does not exist can be found on the WHO Model List of Essential Medicines for Children and in the EMA Inventory of the Needs for Paediatric Medicines. Many of these compounds are off patent, which offers the opportunity for obtaining marketing authorisations for paediatric use. The present study focused on the development of paediatric immediate-release mini-tablet formulations for furosemide. Essential formulation criteria included the use of excipients that are regarded as safe for children, the ease of manufacturing, a high dose flexibility, fast disintegration, a robust drug release and a good acceptability. Only excipients regarded as safe for use in children were used in formulation screening. Compressibility, tablet hardness, disintegration and palatability were the main screening parameters. Formulations with a hardness of  > 20 N, a disintegration time < 3 min (fast disintegration) and a good palatability were selected for mini-tablet production. Based on this pre-assessment two mini-tablet formulations with a furosemide drug load of 2.5 mg were developed. Both were easy to manufacture, had an appropriate hardness, a short disintegration time and met pharmacopoeial requirements with regard to content uniformity and physical testing. Biorelevant in vitro dissolution experiments mimicking different modes (with water or dosing vehicles) of administering age-appropriate furosemide doses to children of different age groups indicated a fast and robust drug release. Overall, the novel mini-tablet formulations present with an increased dose flexibility, excipient safety, swallowability and palatability and are thus a promising starting point for the development of solid oral dosage forms for drugs with paediatric therapeutic needs.", "gemini": "Hơn 10 năm sau khi Quy định về Nhi khoa có hiệu lực, vẫn có nhu cầu lớn về thuốc nhi khoa đối với các hoạt chất thuốc hết bằng sáng chế. Nhiều hợp chất mà không có dạng bào chế cho trẻ em có thể được tìm thấy trong Danh sách Mẫu thuốc thiết yếu của WHO cho trẻ em và trong Hồ sơ nhu cầu thuốc nhi khoa của EMA. Nhiều hợp chất trong số này đã hết bằng sáng chế, tạo cơ hội để xin cấp phép lưu hành cho mục đích sử dụng ở trẻ em. Nghiên cứu hiện tại tập trung vào việc phát triển các dạng bào chế viên nén nhỏ giải phóng tức thì cho furosemide ở trẻ em. Các tiêu chí bào chế thiết yếu bao gồm việc sử dụng các tá dược được coi là an toàn cho trẻ em, dễ sản xuất, linh hoạt về liều lượng, phân rã nhanh, giải phóng thuốc mạnh và được chấp nhận tốt. Chỉ những tá dược được coi là an toàn để sử dụng ở trẻ em mới được sử dụng trong sàng lọc bào chế. Độ nén, độ cứng của viên nén, thời gian phân rã và độ ngon miệng là các thông số sàng lọc chính. Các dạng bào chế có độ cứng> 20 N, thời gian phân rã <3 phút (phân rã nhanh) và độ ngon miệng tốt đã được chọn để sản xuất viên nén nhỏ. Dựa trên đánh giá trước này, hai dạng bào chế viên nén nhỏ với tải thuốc furosemide là 2,5 mg đã được phát triển. Cả hai đều dễ sản xuất, có độ cứng thích hợp, thời gian phân rã ngắn và đáp ứng các yêu cầu của dược điển về tính đồng nhất về hàm lượng và thử nghiệm vật lý. Các thí nghiệm hòa tan trong ống nghiệm có liên quan sinh học mô phỏng các chế độ khác nhau (với nước hoặc phương tiện định liều) để dùng các liều furosemide phù hợp với độ tuổi cho trẻ em ở các nhóm tuổi khác nhau cho thấy giải phóng thuốc nhanh và mạnh. Nhìn chung, các dạng bào chế viên nén nhỏ mới có tính linh hoạt về liều lượng, độ an toàn của tá dược, khả năng nuốt và độ ngon miệng tăng lên và do đó là điểm khởi đầu đầy hứa hẹn cho việc phát triển các dạng bào chế đường uống rắn cho các loại thuốc có nhu cầu điều trị nhi khoa."} +{"idx": 1871175, "en": "The alarming rise in obesity and relative lack of pharmacotherapies to treat, what is becoming a global epidemic, has necessitated that an increasing number of bariatric procedures be performed. Several surgical techniques have been developed during the last 50 years and the advent of laparoscopic surgery has increased the safety and efficacy of these procedures. Bariatric surgery is by a substantial margin, the most efficacious means of achieving sustained weight loss maintenance in patients with obesity. Roux-en-Y gastric bypass surgery (RYGB) elicits the most favourable metabolic outcomes with attendant benefits for type 2 diabetes and, cardiovascular disease as well as endocrine disorders and cancers in females. RYGB is the most extensively studied bariatric procedure regarding mechanism of action. In this review we catalogue the multiple alterations in secretion of gut hormones (ghrelin, obestatin, cholecystokinin, GLP-1, PYY, GIP, oxyntomodulin, glicentin and GLP-2) occurring after RYGB and summarise evidence indicating that these changes play a role in the reduction of food intake and improvements in glucose homeostasis.", "gemini": "Sự gia tăng đáng báo động về tình trạng béo phì và sự thiếu hụt tương đối các liệu pháp điều trị bằng dược lý đối với tình trạng đang trở thành đại dịch toàn cầu này, đã khiến cho ngày càng có nhiều thủ thuật phẫu thuật cắt bỏ dạ dày được thực hiện. Một số kỹ thuật phẫu thuật đã được phát triển trong 50 năm qua và sự ra đời của phẫu thuật nội soi đã làm tăng tính an toàn và hiệu quả của các thủ thuật này. Phẫu thuật cắt bỏ dạ dày là biện pháp hiệu quả nhất để duy trì giảm cân lâu dài ở những bệnh nhân béo phì. Phẫu thuật cắt dạ dày nối tá tràng Roux-en-Y (RYGB) mang lại kết quả chuyển hóa thuận lợi nhất với các lợi ích đi kèm đối với bệnh tiểu đường týp 2 và bệnh tim mạch cũng như các rối loạn nội tiết và ung thư ở phụ nữ. RYGB là thủ thuật phẫu thuật cắt bỏ dạ dày được nghiên cứu sâu rộng nhất về cơ chế hoạt động. Trong bài đánh giá này, chúng tôi lập danh mục nhiều thay đổi trong quá trình tiết hormone đường ruột (ghrelin, obestatin, cholecystokinin, GLP-1, PYY, GIP, oxyntomodulin, glicentin và GLP-2) xảy ra sau RYGB và tóm tắt bằng chứng chỉ ra rằng những thay đổi này đóng một vai trò trong việc giảm lượng thức ăn nạp vào và cải thiện cân bằng glucose."} +{"idx": 1871176, "en": "OBJECTIVE The aim of this study was to: 1) explore older patients' knowledge, skills and behavior in relation to nutrition and 2) achieve an understanding of older patients' experiences, understanding and attitudes towards management of nutritional needs. DESIGN Semi-structured interviews were conducted and analyzed using content analysis. The Readiness and Enablement Index for Health Technology (READHY) instrument was used as a framework. SETTING Two hospital units specialized in internal medicine located at two university hospitals in Copenhagen. PARTICIPANTS Patients (N = 25) age 65 ≥ years and admitted to hospital for medical treatment. FINDINGS The informants' knowledge, behavior and attitude towards nutrition was influenced by their experience of food as an everyday phenomenon but less so by the experience of nutrition as important for their well-being and health. Three themes were identified: 1) Food - an everyday phenomenon; 2) Habits and preferences and 3) When food becomes nutrition. CONCLUSION Older, ill patients have limited knowledge about specific needs for energy and protein and the importance of nutrition for their physical functioning. They have potential resources and competencies which can positively impact and be utilized in nutritional interventions. Social interaction, the pleasurable experience of eating well-prepared food, and daily routines facilitate their nutritional intake.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu này là: 1) tìm hiểu kiến thức, kỹ năng và hành vi của bệnh nhân lớn tuổi liên quan đến dinh dưỡng và 2) hiểu được kinh nghiệm, hiểu biết và thái độ của bệnh nhân lớn tuổi đối với việc quản lý nhu cầu dinh dưỡng. THIẾT KẾ Các cuộc phỏng vấn bán cấu trúc đã được thực hiện và phân tích bằng phân tích nội dung. Công cụ Chỉ số sẵn sàng và khả năng tiếp cận công nghệ sức khỏe (READHY) được sử dụng như một khuôn khổ. BỐI CẢNH Hai đơn vị bệnh viện chuyên khoa nội khoa tại hai bệnh viện đại học ở Copenhagen. NGƯỜI THAM GIA Bệnh nhân (N = 25) từ 65 tuổi trở lên và được nhập viện để điều trị nội khoa. PHÁT HIỆN Kiến thức, hành vi và thái độ của người cung cấp thông tin đối với dinh dưỡng chịu ảnh hưởng bởi kinh nghiệm của họ về thực phẩm như một hiện tượng hàng ngày nhưng ít hơn là kinh nghiệm về dinh dưỡng như một yếu tố quan trọng đối với sức khỏe và sức khỏe của họ. Ba chủ đề đã được xác định: 1) Thực phẩm - một hiện tượng hàng ngày; 2) Thói quen và sở thích và 3) Khi thực phẩm trở thành dinh dưỡng. KẾT LUẬN Những bệnh nhân lớn tuổi, ốm yếu có kiến thức hạn chế về nhu cầu cụ thể về năng lượng và protein cũng như tầm quan trọng của dinh dưỡng đối với chức năng thể chất của họ. Họ có các nguồn lực và năng lực tiềm tàng có thể tác động tích cực và được sử dụng trong các can thiệp dinh dưỡng. Tương tác xã hội, trải nghiệm thú vị khi ăn những món ăn được chế biến kỹ lưỡng và các thói quen hàng ngày tạo điều kiện thuận lợi cho việc hấp thụ dinh dưỡng của họ."} +{"idx": 1871177, "en": "Imaging-guiding and targeted drug delivery to tumors are essential for precision cancer therapy, which is a challenging goal to achieve extraordinary therapeutic efficacy. Functional live cells-based delivery systems are expected to play an important role in imaging-guiding and targeted drug delivery. Herein, we fabricated a delivery system mediated by IR-820 conjugated macrophages for tumor targeted combination therapy under fluorescence imaging guidance. The functional macrophages by nature could cross the barriers and recruit into tumors, and serve as host cells to targeted deliver drugs to tumors. The pH-sensitive doxorubicin nanoparticles were engulfed by the macrophages to enhance the drug loading and decrease the damage on host cells. IR-820 was anchored into macrophages cytoplasm to achieve the dual function of photothermal therapy and fluorescence imaging guidance. With Balb/C mice bearing murine breast tumor (4 T1) as models, the functional macrophages for their innate tumor tropism could targeted transport these therapeutic drugs into tumor site to exert efficient chemo-photothermal combination therapy. Moreover, fluorescence imaging-guided drug delivery was employed as the visible strategy to provide the optimized therapeutic window based on the fluorescence of IR-820. The multi-functional macrophages-mediated delivery system would provide a potential for precise and targeted delivery of combination therapy.", "gemini": "Việc hướng dẫn hình ảnh và đưa thuốc đích đến khối u là rất cần thiết cho liệu pháp ung thư chính xác, đây là một mục tiêu đầy thách thức để đạt được hiệu quả điều trị phi thường. Các hệ thống phân phối dựa trên tế bào sống chức năng dự kiến sẽ đóng một vai trò quan trọng trong việc hướng dẫn hình ảnh và phân phối thuốc đích. Ở đây, chúng tôi chế tạo một hệ thống phân phối được trung gian bởi các đại thực bào liên hợp IR-820 để điều trị kết hợp đích khối u dưới sự hướng dẫn của hình ảnh huỳnh quang. Các đại thực bào chức năng theo bản chất có thể vượt qua các hàng rào và tuyển dụng vào khối u và đóng vai trò là tế bào chủ để đưa thuốc đích đến khối u. Các hạt nano doxorubicin nhạy pH được các đại thực bào nuốt vào để tăng cường tải thuốc và giảm thiểu tổn thương trên tế bào chủ. IR-820 được neo vào tế bào chất của đại thực bào để đạt được chức năng kép là liệu pháp quang nhiệt và hướng dẫn hình ảnh huỳnh quang. Với những con chuột Balb/C mang khối u vú ở chuột (4T1) làm mô hình, các đại thực bào chức năng đối với đặc tính hướng đích khối u bẩm sinh của chúng có thể vận chuyển đích các loại thuốc điều trị này vào vị trí khối u để thực hiện liệu pháp kết hợp hóa chất-quang nhiệt hiệu quả. Hơn nữa, phân phối thuốc hướng dẫn bằng hình ảnh huỳnh quang được sử dụng như một chiến lược hữu hình để cung cấp cửa sổ điều trị tối ưu dựa trên huỳnh quang của IR-820. Hệ thống phân phối được trung gian bởi các đại thực bào đa chức năng sẽ cung cấp tiềm năng phân phối chính xác và có mục tiêu của liệu pháp kết hợp."} +{"idx": 1871178, "en": "Engineering protein and peptide-based materials for drug delivery applications has gained momentum due to their biochemical and biophysical properties over synthetic materials, including biocompatibility, ease of synthesis and purification, tunability, scalability, and lack of toxicity. These biomolecules have been used to develop a host of drug delivery platforms, such as peptide- and protein-drug conjugates, injectable particles, and drug depots to deliver small molecule drugs, therapeutic proteins, and nucleic acids. In this review, we discuss progress in engineering the architecture and biological functions of peptide-based biomaterials -naturally derived, chemically synthesized and recombinant- with a focus on the molecular features that modulate their structure-function relationships for drug delivery.", "gemini": "Kỹ thuật protein và vật liệu dựa trên peptide cho các ứng dụng phân phối thuốc đã đạt được động lực nhờ các đặc tính sinh hóa và sinh lý của chúng so với vật liệu tổng hợp, bao gồm khả năng tương thích sinh học, dễ tổng hợp và tinh chế, khả năng điều chỉnh, khả năng mở rộng và không độc hại. Các phân tử sinh học này đã được sử dụng để phát triển nhiều nền tảng phân phối thuốc, chẳng hạn như liên hợp thuốc-peptide và protein, các hạt có thể tiêm và kho thuốc để phân phối thuốc phân tử nhỏ, protein điều trị và axit nucleic. Trong bài đánh giá này, chúng tôi thảo luận về tiến trình trong việc thiết kế cấu trúc và chức năng sinh học của các vật liệu sinh học dựa trên peptide - có nguồn gốc tự nhiên, tổng hợp hóa học và tái tổ hợp - tập trung vào các đặc điểm phân tử điều chỉnh mối quan hệ cấu trúc-chức năng của chúng để phân phối thuốc."} +{"idx": 1871179, "en": "Studies have shown that the second N-terminal residue of fungal defensins is closely involved in the binding of defensins to lipid II, a bacterial cell wall precursor, and plays an important role in antibacterial activity. We found that the N-terminal residue is always aromatic in nature. In this study, 29 fungal defensin-like peptides were found via the genomic search strategy. Based on the type of aromatic residue at the second N-terminal site, we mainly divided these peptides into Phe, Trp, and Tyr types. We selected and characterized a defensin, blapersin, derived from Blastomyces percursus as a molecular model to investigate the functional significance of the N-terminal site. The native blapersin killed a wide spectrum of gram-positive bacteria at low molecular concentrations. Its aromatic mutants, W2F and W2Y, displayed enhanced antimicrobial activity, especially against the vancomycin-resistant Enterococcus faecium. The aromatic side chains containing Phe2 and Tyr2 seem to be more favorable for the antibacterial activity of blapersin those containing Trp2. However, the nonaromatic mutant W2A had almost no antibacterial activity. This indicates that the second N-terminal aromatic residue is essential for the antimicrobial action of blapersin. All these defensins have high stability and low toxicity. This is the first report on the enhancement of antibacterial activity by calibration of the N-terminal aromatic residue.", "gemini": "Các nghiên cứu đã chỉ ra rằng phần dư N-terminal thứ hai của defensin nấm liên quan chặt chẽ đến sự liên kết của defensin với lipid II, một tiền chất thành tế bào vi khuẩn và đóng vai trò quan trọng trong hoạt động kháng khuẩn. Chúng tôi thấy rằng phần dư N-terminal luôn có bản chất thơm. Trong nghiên cứu này, 29 peptit giống defensin nấm đã được tìm thấy thông qua chiến lược tìm kiếm bộ gen. Dựa trên loại phần dư thơm ở vị trí N-terminal thứ hai, chúng tôi chủ yếu chia các peptit này thành các loại Phe, Trp và Tyr. Chúng tôi đã chọn và đặc trưng hóa một defensin, blapersin, có nguồn gốc từ Blastomyces percursus như một mô hình phân tử để nghiên cứu ý nghĩa chức năng của vị trí N-terminal. Blapersin bản địa đã tiêu diệt nhiều loại vi khuẩn gram dương ở nồng độ phân tử thấp. Các đột biến thơm của nó, W2F và W2Y, thể hiện hoạt tính kháng khuẩn tăng cường, đặc biệt đối với Enterococcus faecium kháng vancomycin. Các chuỗi bên thơm chứa Phe2 và Tyr2 có vẻ thuận lợi hơn cho hoạt động kháng khuẩn của blapersin so với những chuỗi chứa Trp2. Tuy nhiên, đột biến không thơm W2A hầu như không có hoạt tính kháng khuẩn. Điều này chỉ ra rằng phần dư thơm N-terminal thứ hai rất cần thiết cho tác dụng kháng khuẩn của blapersin. Tất cả các defensin này đều có độ ổn định cao và độc tính thấp. Đây là báo cáo đầu tiên về việc tăng cường hoạt tính kháng khuẩn bằng cách hiệu chuẩn phần dư thơm N-terminal."} +{"idx": 1871180, "en": "Persistent infections caused by high-risk human papillomavirus (HR-HPV) are important, for the development of cervical lesions, but environmental and genetic factors are also related in the process of carcinogenesis. Among the genetic factors, the genetic variants of HR-HPV appear to be related to the risk of persistent infections. Therefore, the present study investigates variants of HPV31 E5 oncogene in cervical scraping samples from Brazilian women to assess their functional and structural effects, in order to identify possible repercussions of these variants on the infectious and carcinogenic process. Our results detected nucleotide changes previously described in the HPV31 E5 oncogene, which may play a critical role in the development of cancer due to its ability to promote cell proliferation and signal transmission. In our study, the interaction percentage of the 31E5 sequence generated by the Immune Epitope Server database and the Analysis Resource (IEDB) allowed us to include possible immunogenic epitopes with the MHC-I and MHC-II molecules, which may represent a possible relationship between protein suppression of the immune system. In the structural analysis of the HPV31 E5 oncoprotein, the N5D, I48 V, P56A, F80I and V64I polymorphisms can be found inserted within transmembrane regions. The P56A mutation has been predicted to be highly stabilizing and, therefore, can cause a change in protein function. Regarding the interaction of the E5 protein from HPV31 with the signaling of NF-kB pathway, we observed that in all variants of the E5 gene from HPV-31, the activity of the NF-kB pathway was increased compared to the prototype. Our study contributes to a more refined design of studies with the E5 gene from HPV31 and provides important data for a better understanding of how variants can be distinguished under their clinical consequences.", "gemini": "Nhiễm trùng dai dẳng do virus papilloma ở người nguy cơ cao (HR-HPV) rất quan trọng đối với sự phát triển của các tổn thương cổ tử cung, nhưng các yếu tố môi trường và di truyền cũng có liên quan đến quá trình gây ung thư. Trong số các yếu tố di truyền, các biến thể di truyền của HR-HPV dường như liên quan đến nguy cơ nhiễm trùng dai dẳng. Do đó, nghiên cứu hiện tại điều tra các biến thể của oncogen HPV31 E5 trong các mẫu cạo cổ tử cung từ phụ nữ Brazil để đánh giá các tác động về chức năng và cấu trúc của chúng, nhằm xác định hậu quả có thể xảy ra của các biến thể này đối với quá trình nhiễm trùng và gây ung thư. Kết quả của chúng tôi phát hiện ra những thay đổi về nucleotide đã được mô tả trước đó trong oncogen HPV31 E5, có thể đóng vai trò quan trọng trong sự phát triển của ung thư do khả năng thúc đẩy sự phát triển của tế bào và truyền tín hiệu. Trong nghiên cứu của chúng tôi, tỷ lệ tương tác của chuỗi 31E5 do cơ sở dữ liệu Máy chủ Epitop Miễn dịch và Tài nguyên Phân tích (IEDB) tạo ra cho phép chúng tôi đưa vào các epitop miễn dịch có thể có với các phân tử MHC-I và MHC-II, có thể thể hiện mối quan hệ có thể xảy ra giữa protein ức chế hệ thống miễn dịch. Trong phân tích cấu trúc của oncoprotein HPV31 E5, các đa hình N5D, I48 V, P56A, F80I và V64I có thể được tìm thấy được chèn vào trong các vùng xuyên màng. Đột biến P56A được dự đoán là có độ ổn định cao và do đó có thể gây ra sự thay đổi chức năng protein. Về sự tương tác của protein E5 từ HPV31 với tín hiệu của con đường NF-kB, chúng tôi quan sát thấy rằng ở tất cả các biến thể của gen E5 từ HPV-31, hoạt động của con đường NF-kB tăng lên so với kiểu mẫu. Nghiên cứu của chúng tôi góp phần thiết kế tinh tế hơn các nghiên cứu với gen E5 từ HPV31 và cung cấp dữ liệu quan trọng để hiểu rõ hơn về cách phân biệt các biến thể theo hậu quả lâm sàng của chúng."} +{"idx": 1871181, "en": "Pronounced heterothermic responses are relatively rare among birds. Along with taxa such as hummingbirds and caprimulgids, the order Coliiformes (mousebirds) is known to possess the physiological capacity for torpor. During torpor, body temperature is greatly reduced and a bird becomes unresponsive to external stimuli until ambient temperatures return to more favorable conditions. Under such conditions, these birds are forced to rely only on their internal fuel storage for energy and show great reduction in metabolic rates by decreasing energy-expensive processes. This study investigated the role of the key insulin-Akt signaling kinase pathway involved in regulating energy metabolism and protein translation in the liver, kidney, heart, skeletal muscle, and brain of the speckled mousebird (Colius striatus). The degree of phosphorylation of well-conserved target residues with important regulatory function was examined in both the euthermic control and torpid birds. The results demonstrated marked differences in responses between the tissues with decreases in RPS6 S235/236 phosphorylation in the kidney (0.52 fold of euthermic) and muscle (0.29 fold of euthermic) as well as decreases in GS3K3β S9 in muscle (0.60 fold of euthermic) and GSK3α S21 (0.71 fold of euthermic) phosphorylation in kidney during torpor, suggesting a downregulation of this pathway. Interestingly, the liver demonstrated an increase in RPS6 S235/236 (2.89 fold increase) and P70S6K T412 (1.44 fold increase) phosphorylation in the torpor group suggesting that protein translation is maintained in this tissue. This study demonstrates that avian torpor is a complex phenomenon and alterations in this signaling pathway follow a tissue specific pattern.", "gemini": "Phản ứng dị nhiệt rõ rệt tương đối hiếm ở chim. Cùng với các loài như chim ruồi và họ Caprimulgidae, bộ Coliiformes (chim chuột) được biết là có khả năng sinh lý đối với trạng thái tê cứng. Trong trạng thái tê cứng, nhiệt độ cơ thể giảm mạnh và chim không phản ứng với các kích thích bên ngoài cho đến khi nhiệt độ môi trường trở lại điều kiện thuận lợi hơn. Trong những điều kiện như vậy, những con chim này buộc phải chỉ dựa vào nguồn dự trữ nhiên liệu bên trong để lấy năng lượng và cho thấy sự giảm đáng kể về tỷ lệ trao đổi chất bằng cách giảm các quá trình tiêu tốn nhiều năng lượng. Nghiên cứu này đã điều tra vai trò của con đường kinase tín hiệu insulin-Akt quan trọng liên quan đến việc điều chỉnh quá trình trao đổi chất năng lượng và dịch mã protein ở gan, thận, tim, cơ xương và não của chim chuột đốm (Colius striatus). Mức độ phosphoryl hóa của các phần dư mục tiêu được bảo tồn tốt với chức năng điều hòa quan trọng đã được kiểm tra ở cả chim kiểm soát nhiệt và chim tê cứng. Kết quả cho thấy sự khác biệt rõ rệt về phản ứng giữa các mô với sự giảm phosphoryl hóa RPS6 S235/236 ở thận (0,52 lần so với nhiệt) và cơ (0,29 lần so với nhiệt) cũng như sự giảm GS3K3β S9 ở cơ (0,60 lần so với nhiệt) và phosphoryl hóa GSK3α S21 (0,71 lần so với nhiệt) ở thận trong trạng thái tê cứng, cho thấy sự điều hòa giảm của con đường này. Điều thú vị là gan cho thấy sự gia tăng phosphoryl hóa RPS6 S235/236 (tăng 2,89 lần) và P70S6K T412 (tăng 1,44 lần) trong nhóm tê cứng, cho thấy quá trình dịch mã protein được duy trì trong mô này. Nghiên cứu này chứng minh rằng trạng thái tê cứng ở chim là một hiện tượng phức tạp và những thay đổi trong con đường tín hiệu này tuân theo một mô hình cụ thể."} +{"idx": 1871182, "en": "Signal transducers and activators of transcription 3 (STAT3) is reported to regulate cell proliferation, survival, and differentiation, and thus plays a central role in development and carcinogenesis. Accumulating evidence demonstrated the involvement of cellular Src (c-Src) tyrosine kinase in the activation of STAT3. Additionally, novel oncogene with kinase-domain (NOK), a receptor protein tyrosine kinase that involves in cell transformation and tumorigenesis, was found to activate STAT3 signaling by a JAK2-dependent mechanism. However, whether the existence of the interaction between c-Src/STAT3 and NOK/STAT3 signals is still unknown. In this study, we showed that NOK formed a complex with c-Src and facilitated the interaction between c-Src and STAT3. In the complex, NOK greatly elevated the c-Src-mediated STAT3 activation by increasing the phosphorylation level of STAT3 on Tyr705. Truncated and mutation experiments further demonstrated that the kinase activity was responsible for the synergistic effect of NOK and c-Src on STAT3 activation. In addition, NOK and c-Src synergistically promoted cell proliferation and tumor growth in nude mice. Taken together, our results indicate that NOK associates with c-Src and promotes c-Src-induced STAT3 activation in a kinase-dependent manner. We proposed that the axis that NOK promoted c-Src-induced STAT3 activation is critical in cell proliferation and tumorigenesis.", "gemini": "Chất truyền tín hiệu và hoạt hóa phiên mã 3 (STAT3) được báo cáo là điều chỉnh sự tăng sinh, sự sống sót và sự biệt hóa của tế bào, do đó đóng vai trò trung tâm trong sự phát triển và gây ung thư. Bằng chứng tích lũy đã chứng minh sự tham gia của tyrosine kinase Src tế bào (c-Src) trong quá trình hoạt hóa STAT3. Ngoài ra, oncogen mới có miền kinase (NOK), một thụ thể protein tyrosine kinase liên quan đến quá trình chuyển đổi tế bào và gây ung thư, được phát hiện là hoạt hóa tín hiệu STAT3 theo cơ chế phụ thuộc JAK2. Tuy nhiên, sự tồn tại của tương tác giữa tín hiệu c-Src/STAT3 và NOK/STAT3 vẫn chưa được biết. Trong nghiên cứu này, chúng tôi đã chỉ ra rằng NOK tạo thành một phức hợp với c-Src và tạo điều kiện cho sự tương tác giữa c-Src và STAT3. Trong phức hợp, NOK làm tăng đáng kể sự hoạt hóa STAT3 do c-Src trung gian bằng cách tăng mức độ phosphoryl hóa của STAT3 trên Tyr705. Các thí nghiệm cắt cụt và đột biến cho thấy hoạt động của kinase chịu trách nhiệm cho tác dụng hiệp đồng của NOK và c-Src đối với hoạt hóa STAT3. Ngoài ra, NOK và c-Src thúc đẩy sự tăng sinh tế bào và phát triển khối u ở chuột nude theo cách hiệp đồng. Tổng hợp lại, kết quả của chúng tôi chỉ ra rằng NOK liên kết với c-Src và thúc đẩy sự hoạt hóa STAT3 do c-Src gây ra theo cách phụ thuộc kinase. Chúng tôi đề xuất rằng trục mà NOK thúc đẩy sự hoạt hóa STAT3 do c-Src gây ra là rất quan trọng trong sự tăng sinh tế bào và gây ung thư."} +{"idx": 1871183, "en": "Antibiotic resistance genes are often found in structures bounded by copies of IS26, IS257/IS431 or IS1216 that resemble compound (or composite) transposons. However, because of the mechanisms used by IS26 family members, namely that they form cointegrates but cannot resolve them, none of these structures can move together as a coherent single unit. Apparent transposition of these structures is possible via a 2-step process but only if the IS are in direct orientation. An intermolecular reaction catalysed by the IS-encoded transposase and an intramolecular homologous recombination step can occur in either order. In one route, one of the IS bounding the structure forms a cointegrate between the DNA molecule carrying it and a target molecule. Cointegrates formed by either copy-in or targeted conservative routes contain three directly-oriented IS copies and can be resolved by homologous recombination between specific pairs of IS, with one pair leading to apparent transposition of the whole structure. In the other route, homologous recombination first forms a circular intermediate, a translocatable unit or TU, which is incorporated by the transposase either at a random site or adjacent to another IS copy in a target molecule. We therefore conclude that the transposon-like structures are not compound (or composite) transposons and the nomenclature for them should be revised. We propose that the term \"pseudo compound transposon\" (PCT), first coined in 1989, should be used to describe those structures where the IS are in direct orientation. Structures with the IS in opposite orientation should not be named as transposons.", "gemini": "Các gen kháng kháng sinh thường được tìm thấy trong các cấu trúc bị giới hạn bởi các bản sao của IS26, IS257/IS431 hoặc IS1216 giống như các transposon kép (hoặc hỗn hợp). Tuy nhiên, do cơ chế được các thành viên của họ IS26 sử dụng, cụ thể là chúng tạo thành các hợp nhất nhưng không thể giải quyết chúng, không có cấu trúc nào trong số các cấu trúc này có thể di chuyển cùng nhau như một đơn vị duy nhất mạch lạc. Sự chuyển vị rõ ràng của các cấu trúc này có thể thông qua một quá trình 2 bước nhưng chỉ khi các IS ở hướng trực tiếp. Phản ứng liên phân tử do transposase được mã hóa bởi IS xúc tác và một bước tái tổ hợp tương đồng nội phân tử có thể xảy ra theo bất kỳ thứ tự nào. Trong một con đường, một trong những IS giới hạn cấu trúc tạo thành một hợp nhất giữa phân tử DNA mang nó và một phân tử đích. Các hợp nhất được hình thành bởi các con đường bảo thủ sao chép hoặc nhắm mục tiêu chứa ba bản sao IS hướng trực tiếp và có thể được giải quyết bằng tái tổ hợp tương đồng giữa các cặp IS cụ thể, với một cặp dẫn đến sự chuyển vị rõ ràng của toàn bộ cấu trúc. Trong con đường khác, tái tổ hợp tương đồng đầu tiên tạo thành một trung gian hình tròn, một đơn vị có thể dịch chuyển hoặc TU, được transposase kết hợp ở một vị trí ngẫu nhiên hoặc cạnh một bản sao IS khác trong phân tử đích. Do đó, chúng tôi kết luận rằng các cấu trúc giống như transposon không phải là transposon kép (hoặc hỗn hợp) và thuật ngữ cho chúng nên được sửa đổi. Chúng tôi đề xuất rằng thuật ngữ \"transposon kép giả\" (PCT), lần đầu tiên được đặt ra vào năm 1989, nên được sử dụng để mô tả những cấu trúc mà các IS ở hướng trực tiếp. Các cấu trúc có IS ở hướng ngược nhau không nên được gọi là transposon."} +{"idx": 1871184, "en": "From a pharmaceutical standpoint, cyclodextrin-based products have deservedly gained substantial market share due to their ability to improve undesirable physicochemical properties of drugs. In this study the solubility of a potenial antifungal compound (L-173) has been improved essentially by addition of β-cyclodextrin (β-CD), 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), and heptakis(2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD) in aqueous solutions (pH 2.0 and pH 7.4) at 298.15-313.15 K. The phase solubility diagrams were constructed. The stoichiometric ratio of the complexes was determined as 1:1. The stability constants of L-173 with all three CDs in acidic medium belong to the range optimal for the improvement of the bioavailability of hydrophobic drugs. DM-β-CD was assigned as the best solubilizer for L-173. The driving forces of the solubilization and complexation process were revealed by evaluating the thermodynamic parameters. The distribution behavior of L-173 in the 1-octanol/buffer and 1-hexane buffer systems at pH 2.0 and pH 7.4 in the presence of different CDs concentrations was studied. The reduction of the distribution coefficients with the increasing of CD concentration was detected due to complex formation. Based on the analysis of the solubility-distribution relationship, the L-173 partitioning between the biological tissues and penetration through the biological membranes in case when cyclodextrins are used as solubilizers was evaluated, and the optimal CD concentrations were proposed.", "gemini": "Theo quan điểm dược phẩm, các sản phẩm dựa trên cyclodextrin xứng đáng giành được thị phần đáng kể do khả năng cải thiện các tính chất lý hóa không mong muốn của thuốc. Trong nghiên cứu này, độ hòa tan của một hợp chất chống nấm tiềm năng (L-173) đã được cải thiện về cơ bản bằng cách bổ sung β-cyclodextrin (β-CD), 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) và heptakis(2,6-di-O-methyl)-β-cyclodextrin (DM-β-CD) trong dung dịch nước (pH 2,0 và pH 7,4) ở 298,15-313,15 K. Biểu đồ độ hòa tan theo pha đã được xây dựng. Tỷ lệ thành phần của các phức hợp được xác định là 1:1. Hằng số ổn định của L-173 với cả ba CD trong môi trường axit nằm trong phạm vi tối ưu để cải thiện khả dụng sinh học của thuốc kỵ nước. DM-β-CD được chỉ định là chất hòa tan tốt nhất cho L-173. Các lực đẩy của quá trình hòa tan và phức hợp hóa đã được tiết lộ bằng cách đánh giá các thông số nhiệt động lực học. Hành vi phân phối của L-173 trong hệ đệm 1-octanol/đệm và 1-hexan ở pH 2,0 và pH 7,4 khi có mặt các nồng độ CD khác nhau đã được nghiên cứu. Sự giảm hệ số phân phối khi nồng độ CD tăng được phát hiện do sự hình thành phức hợp. Dựa trên phân tích mối quan hệ hòa tan-phân phối, sự phân bố L-173 giữa các mô sinh học và sự thâm nhập qua màng sinh học trong trường hợp sử dụng cyclodextrin làm chất hòa tan đã được đánh giá và các nồng độ CD tối ưu đã được đề xuất."} +{"idx": 1871185, "en": "Four formulations of nanostructured lipid carriers (NLC) loaded with curcuminoids where prepared, testing two types of solid lipids (Compritol® 888 ATO and Precirol® ATO 5) and two kinds of stabilizers (poloxamer 407 and polysorbate 80). Particle size values between 111 and 214 nm and polydispersity indices < 0.3 were registered, with low Z potential values due to the nonionic character of the stabilizers. The results showed that the type of surfactant had an impact on the in vitro release rate and on the ex vivo skin permeation capability of curcuminoids. Polysorbate 80 delayed the release, but favors the transport of a higher amount of curcuminoids to the receptor solution during the ex vivo permeation studies than the systems with poloxamer 407. Confocal microscopy confirmed that all systems favored the penetration of curcuminoids to deeper layers of the skin and in a greater amount than curcuminoids in solution. Exposure of the systems to intense radiation caused the degradation of curcuminoids, without loss of antioxidant activity, confirming that the degradation products also function as antioxidants. The NLC prepared can be valuable carriers to enhance the penetration of curcuminoids into the skin, to treat different disorders and skin diseases.", "gemini": "Bốn công thức của chất mang lipid có cấu trúc nano (NLC) chứa curcuminoid đã được chuẩn bị, thử nghiệm hai loại lipid rắn (Compritol® 888 ATO và Precirol® ATO 5) và hai loại chất ổn định (poloxamer 407 và polysorbate 80). Các giá trị kích thước hạt nằm trong khoảng từ 111 đến 214 nm và chỉ số phân tán poly < 0,3 đã được ghi lại, với các giá trị điện thế Z thấp do đặc tính không ion của chất ổn định. Kết quả cho thấy loại chất hoạt động bề mặt có tác động đến tốc độ giải phóng in vitro và khả năng thẩm thấu qua da ex vivo của curcuminoid. Polysorbate 80 làm chậm quá trình giải phóng, nhưng lại ủng hộ việc vận chuyển một lượng curcuminoid cao hơn đến dung dịch thụ thể trong quá trình thẩm thấu ex vivo so với các hệ thống có poloxamer 407. Kính hiển vi cộng hưởng xác nhận rằng tất cả các hệ thống đều ủng hộ sự thâm nhập của curcuminoid vào các lớp sâu hơn của da và với lượng lớn hơn so với curcuminoid trong dung dịch. Việc tiếp xúc các hệ thống với bức xạ mạnh gây ra sự phân hủy của curcuminoid, mà không làm mất hoạt tính chống oxy hóa, xác nhận rằng các sản phẩm phân hủy cũng hoạt động như chất chống oxy hóa. NLC được chế tạo có thể là chất mang có giá trị để tăng cường sự thâm nhập của curcuminoid vào da, để điều trị các rối loạn và bệnh ngoài da khác nhau."} +{"idx": 1871186, "en": "It is our hypothesis that the presence of an absorptive sink for in-vitro dissolution experiments is decisive to predict extent and duration of super-saturation of low soluble drugs in formulations expected to increase oral absorption, often called enabling formulations. Combined dissolution-/permeation-testing may provide such absorptive sink. Commonly used in-vitro dissolution-/permeation tools have a limited interfacial area-to-donor-volume-ratio (A/V), far below the physiological one which is estimated for humans. In consequence, super-saturation is expected to be more pronounced and thus precipitation to occur more readily in these models as compared to the in-vivo situation. In the current study, a PermeaLoop™ prototype a of a novel in-vitro dissolution-/permeation-tool with a substantially larger A/V was employed to investigate the dissolution and permeation behaviour of model formulations of dipyridamole containing fumaric acid as modifier of the micro-environmental pH. After identifying the most suitable experimental conditions in terms of donor- and acceptor pH and composition, dose, flow-rate and sampling intervals, both the dissolution and the permeation were simultaneously assessed over time and the extent and duration of super-saturation monitored. The importance of biomimetic media in the donor was revealed not only in terms of increasing the dissolution but also the permeation. The formulations were ranked in terms of their performance (cumulative amount permeated). As a result the data generated by PermeaLoop experiments showed for the same formulations a superior correlation with in rat bioavailability data than obtained from a traditional side-by-side Dissolution-/Permeation-system with a Caco-2-cell membrane (D/P-system). The insights into the effects of solubilisers and pH conditions gained in the present study contribute to an improved mechanistic understanding of dynamic dissolution/permeation behaviour of weakly basic drugs and their enabling formulations. Challenges with the current PermeaLoop prototype are still to be solved, as dispersed drug still tends to get stuck inside the system, but gained experiences are helpful for the improvement of the design.", "gemini": "Giả thuyết của chúng tôi là sự hiện diện của một bồn chứa hấp thụ cho các thí nghiệm hòa tan trong ống nghiệm có tính quyết định trong việc dự đoán mức độ và thời gian quá bão hòa của các loại thuốc ít tan trong các công thức dự kiến làm tăng hấp thu đường uống, thường được gọi là các công thức hỗ trợ. Thử nghiệm hòa tan kết hợp / thẩm thấu có thể cung cấp bồn chứa hấp thụ như vậy. Các công cụ hòa tan / thẩm thấu trong ống nghiệm thường được sử dụng có tỷ lệ diện tích giao diện / thể tích của người hiến tặng (A / V) hạn chế, thấp hơn nhiều so với tỷ lệ sinh lý được ước tính cho con người. Do đó, quá bão hòa dự kiến sẽ rõ rệt hơn và do đó quá trình kết tủa xảy ra dễ dàng hơn trong các mô hình này so với tình hình trong cơ thể. Trong nghiên cứu hiện tại, một nguyên mẫu PermeaLoop ™ của một công cụ hòa tan / thẩm thấu trong ống nghiệm mới có A / V lớn hơn đáng kể đã được sử dụng để nghiên cứu hành vi hòa tan và thẩm thấu của các công thức mô hình của dipyridamole có chứa axit fumaric làm chất điều chỉnh pH vi mô. Sau khi xác định các điều kiện thực nghiệm phù hợp nhất về độ pH và thành phần của người hiến tặng và người nhận, liều, tốc độ dòng chảy và khoảng thời gian lấy mẫu, cả quá trình hòa tan và thẩm thấu đều được đánh giá đồng thời theo thời gian và mức độ và thời gian của quá bão hòa được theo dõi. Tầm quan trọng của môi trường sinh học trong người hiến tặng được tiết lộ không chỉ về mặt tăng khả năng hòa tan mà còn cả khả năng thẩm thấu. Các công thức được xếp hạng theo hiệu suất của chúng (lượng thẩm thấu tích lũy). Kết quả là dữ liệu thu được từ các thí nghiệm PermeaLoop cho thấy đối với cùng một công thức có tương quan cao hơn với dữ liệu sinh khả dụng trên chuột so với dữ liệu thu được từ hệ thống hòa tan / thẩm thấu cạnh nhau truyền thống có màng tế bào Caco-2 (hệ thống D / P ). Những hiểu biết sâu sắc về tác động của các chất hòa tan và điều kiện pH thu được trong nghiên cứu hiện tại góp phần cải thiện sự hiểu biết về cơ chế của hành vi hòa tan / thẩm thấu động của các loại thuốc có tính bazơ yếu và các công thức hỗ trợ của chúng. Những thách thức với nguyên mẫu PermeaLoop hiện tại vẫn cần được giải quyết, vì thuốc phân tán vẫn có xu hướng bị kẹt bên trong hệ thống, nhưng những kinh nghiệm thu được rất hữu ích cho việc cải tiến thiết kế."} +{"idx": 1871187, "en": "BACKGROUND Numerous microRNAs (miRNAs) have been investigated in the progression of Alzheimer's disease (AD). The purpose of this study was to analyze the expression of miR-433 and its diagnostic value in patients with AD, and to explore the neuroprotective effect of miR-433 in amyloid β (Aβ)-treated SH-SY5Y and SK-N-SH cells. METHODS AD patients and AD cell model that established by Aβ treatment were used in this study. Quantitative real-time PCR was used to measure the expression of miR-433. The diagnostic value of miR-433 was evaluated using the receiver operating characteristic analysis. MTT assay was used to examine the viability of Aβ-treated SH-SY5Y and SK-N-SH cells. Bioinformatics and luciferase activity analyses were used to confirm the target gene that might be involved in the mechanisms of miR-433 in AD. RESULTS Expression levels of miR-433 were decreased in AD patients and cells compared with the corresponding controls. The decreased miR-433 expression levels in serum and cerebrospinal fluid (CS) were positively correlated with MMSE scores and had relatively high diagnostic accuracy in AD patients. The gain-of-function experiments found that the overexpression of miR-433 could rescue the Aβ-induced inhibition in neuronal viability in SH-SY5Y and SK-N-SH cells. The luciferase activity results showed that JAK2 was a target gene of miR-433 in neuronal cells. CONCLUSION All the data of this study showed that miR-433 serves as a candidate diagnostic biomarker for AD patients, and may have the potential as a novel therapeutic target by ameliorating Aβ-induced neurotoxicity.", "gemini": "LÝ LỊCH Nhiều microRNA (miRNA) đã được nghiên cứu trong quá trình tiến triển của bệnh Alzheimer (AD). Mục đích của nghiên cứu này là phân tích biểu hiện của miR-433 và giá trị chẩn đoán của nó ở những bệnh nhân mắc AD, đồng thời khám phá tác dụng bảo vệ thần kinh của miR-433 trong tế bào SH-SY5Y và SK-N-SH được xử lý bằng amyloid β (Aβ). PHƯƠNG PHÁP Bệnh nhân AD và mô hình tế bào AD được thiết lập bằng cách điều trị Aβ đã được sử dụng trong nghiên cứu này. Phản ứng PCR định lượng thời gian thực được sử dụng để đo biểu hiện của miR-433. Giá trị chẩn đoán của miR-433 được đánh giá bằng cách sử dụng phân tích đặc điểm hoạt động của người nhận. Xét nghiệm MTT được sử dụng để kiểm tra khả năng sống của các tế bào SH-SY5Y và SK-N-SH được xử lý bằng Aβ. Phân tích sinh tin học và hoạt động luciferase được sử dụng để xác nhận gen đích có thể liên quan đến cơ chế của miR-433 trong AD. KẾT QUẢ Mức độ biểu hiện của miR-433 giảm ở bệnh nhân AD và tế bào so với nhóm đối chứng tương ứng. Mức độ biểu hiện miR-433 giảm trong huyết thanh và dịch não tủy (CS) có tương quan thuận với điểm MMSE và có độ chính xác chẩn đoán tương đối cao ở bệnh nhân AD. Các thí nghiệm tăng chức năng phát hiện ra rằng sự biểu hiện quá mức của miR-433 có thể cứu vãn sự ức chế do Aβ gây ra đối với khả năng sống của tế bào thần kinh ở các tế bào SH-SY5Y và SK-N-SH. Kết quả hoạt động của luciferase cho thấy JAK2 là gen đích của miR-433 trong tế bào thần kinh. KẾT LUẬN Tất cả dữ liệu của nghiên cứu này cho thấy miR-433 đóng vai trò là một ứng cử viên sinh học chẩn đoán cho bệnh nhân AD và có thể có tiềm năng trở thành mục tiêu điều trị mới bằng cách cải thiện độc tính thần kinh do Aβ gây ra."} +{"idx": 1871188, "en": "This work aims to develop complimentary analytical tools for lipid formulation selection that offer insights into the mechanisms of in-vitro drug release for solid lipid modified release excipients. Such tools are envisioned to aide and expedite the time consuming process of formulation selection and development. Two pharmaceutically relevant solid lipid excipients are investigated, stearyl alcohol and glyceryl behenate, which are generally known to exhibit faster and slower relative release rates, respectively. Nuclear magnetic resonance spectroscopy and diffusometry are used, along with water uptake and dissolution experiments to help distinguish between two proposed in-vitro release mechanisms for crystalline caffeine from these matrices: 1) rate limiting movement of the wetting front through the particle, and 2) rate limiting diffusive release of the active from the wetted particle. Findings based on water permeation rates, API diffusion coefficients and kinetic modeling suggest that the rate limiting steps for caffeine release from these matrices are different, with stearyl alcohol being co-rate limited by movement of the wetting front and diffusive release of API, whereas glyceryl behenate is more strictly limited by diffusive release of API from the wetted matrix. A Peclet-like number is proposed to describe the different regimes of rate limitation for drug release. NMR spectroscopy and diffusometry are demonstrated to be useful tools for elucidating mechanisms of API release from crystalline drug/lipid mixtures and have significant potential value as screening tools in MR formulation development.", "gemini": "Nghiên cứu này nhằm mục đích phát triển các công cụ phân tích bổ sung để lựa chọn công thức lipid, cung cấp thông tin chi tiết về cơ chế giải phóng thuốc trong ống nghiệm cho các tá dược giải phóng lipid rắn đã được sửa đổi. Các công cụ như vậy được hình dung để hỗ trợ và đẩy nhanh quá trình tốn thời gian của việc lựa chọn và phát triển công thức. Hai tá dược lipid rắn có liên quan đến dược phẩm được nghiên cứu, đó là rượu stearyl và glyceryl behenate, thường được biết là có tốc độ giải phóng tương đối nhanh hơn và chậm hơn. Phổ cộng hưởng từ hạt nhân và phép khuếch tán được sử dụng, cùng với các thí nghiệm hấp thụ nước và hòa tan để giúp phân biệt giữa hai cơ chế giải phóng trong ống nghiệm được đề xuất đối với caffeine dạng tinh thể từ các ma trận này: 1) chuyển động giới hạn tốc độ của mặt trận làm ướt qua hạt và 2) giới hạn tốc độ giải phóng khuếch tán của hoạt chất từ hạt đã làm ướt. Các phát hiện dựa trên tốc độ thấm nước, hệ số khuếch tán API và mô hình động học cho thấy các bước giới hạn tốc độ giải phóng caffeine từ các ma trận này là khác nhau, trong đó rượu stearyl bị giới hạn cùng tốc độ do chuyển động của mặt trận làm ướt và giải phóng khuếch tán của API, trong khi glyceryl behenate bị giới hạn nghiêm ngặt hơn do giải phóng khuếch tán của API từ ma trận đã làm ướt. Một số Peclet được đề xuất để mô tả các chế độ giới hạn tốc độ khác nhau để giải phóng thuốc. Phổ NMR và phép khuếch tán được chứng minh là những công cụ hữu ích để làm sáng tỏ cơ chế giải phóng API từ hỗn hợp thuốc/lipid dạng tinh thể và có giá trị tiềm năng đáng kể như các công cụ sàng lọc trong phát triển công thức MR."} +{"idx": 1871189, "en": "Amphotericin B (AmB) is an effective antifungal agent for life-threatening systemic fungal infections. However, its poor solubility in water and organic solvents makes it difficult to formulate. We previously reported AmB-encapsulated micellar formations using styrene-maleic acid copolymer (SMA) and butylated SMA. These micelles make AmB water-soluble; however, the blood circulation of AmB by these intravenous administrations was as low as that of Fungizone®, a conventional micellar formulation of AmB. The destabilization of SMA micelles by salt in the blood has been suggested to be a cause of low blood circulation. Therefore, in this study, to reduce salt-induced instability and increase blood circulation of the micelles, we covalently attached cholesterol molecules to the SMA backbone because AmB interacts with sterols. This AmB nanoparticle micellar formulation (Cho-SMA/AmB micelles) was water-soluble, stable in the presence of salts, and formed a complex with albumin. Compared with Fungizone®, this formulation had equal antifungal activity and markedly improved blood circulation and lower toxicity. Its toxicity was further reduced in the presence of albumin. Taken together, our results indicate that Cho-SMA/AmB micelles could be an intravenous formulation with high antifungal selectivity, and drug interactants-conjugated SMA system could be applied to a variety of drug-loaded nanomicellar systems.", "gemini": "Amphotericin B (AmB) là một chất chống nấm hiệu quả đối với các bệnh nhiễm nấm toàn thân đe dọa tính mạng. Tuy nhiên, độ hòa tan kém trong nước và dung môi hữu cơ khiến nó khó tạo thành công thức. Trước đây chúng tôi đã báo cáo về các chế phẩm micelle chứa AmB bằng cách sử dụng đồng trùng hợp styrene-maleic acid (SMA) và SMA butyl hóa. Các micelle này làm cho AmB tan trong nước; tuy nhiên, tuần hoàn máu của AmB bằng các chế phẩm tiêm tĩnh mạch này thấp như Fungizone®, một chế phẩm micelle thông thường của AmB. Sự bất ổn định của micelle SMA do muối trong máu được cho là nguyên nhân gây ra tuần hoàn máu thấp. Do đó, trong nghiên cứu này, để giảm sự bất ổn định do muối gây ra và tăng tuần hoàn máu của micelle, chúng tôi đã liên kết cộng hóa trị các phân tử cholesterol với xương sống SMA vì AmB tương tác với sterol. Công thức micelle nano AmB này (micelle Cho-SMA/AmB) tan trong nước, ổn định khi có muối và tạo thành phức hợp với albumin. So với Fungizone®, công thức này có hoạt tính chống nấm tương đương và cải thiện đáng kể tuần hoàn máu và độc tính thấp hơn. Độc tính của nó được giảm thêm khi có albumin. Kết hợp lại, kết quả của chúng tôi chỉ ra rằng micelle Cho-SMA/AmB có thể là một công thức tiêm tĩnh mạch có tính chọn lọc chống nấm cao và hệ thống SMA liên hợp với chất tương tác thuốc có thể được áp dụng cho nhiều hệ thống micelle nano chứa thuốc."} +{"idx": 1871190, "en": "In this study, a novel injectable hydrogel based on iota and kappa carrageenan, locust bean gum and gelatin was prepared for wound healing and tissue repairing applications. This injectable hydrogel was obtained via physical crosslinking. FTIR analysis confirmed the physical interaction between the biopolymeric components of the hydrogel. The prepared injectable hydrogel exhibited shear-thinning characteristics and could be injected for minimally invasive applications. Also, the hydrogel showed a porous structure, physiological and mechanical stability and biocompatibility. The in vitro cell culture studies showed that fibroblasts were able to grow, adhere and spread inside the hydrogel, indicating that hydrogel could support tissue repair. Moreover, hydrogel could be useful for the delivery of biomolecules. Vascular endothelial growth factor was encapsulated within the hydrogel and subsequently released, which accelerated the migration of human umbilical vein endothelial cells and facilitated in vitro wound healing. Overall, the results indicate that hydrogel can be a potential injectable delivery vehicle for wound healing and tissue repair.", "gemini": "Trong nghiên cứu này, một loại hydrogel tiêm mới dựa trên iota và kappa carrageenan, kẹo cao su đậu carob và gelatin đã được chế tạo để chữa lành vết thương và ứng dụng sửa chữa mô. Hydrogel tiêm này được tạo ra thông qua liên kết chéo vật lý. Phân tích FTIR xác nhận sự tương tác vật lý giữa các thành phần biopolymer của hydrogel. Hydrogel tiêm đã chuẩn bị thể hiện các đặc tính làm loãng theo độ cắt và có thể được tiêm để sử dụng ít xâm lấn. Ngoài ra, hydrogel cho thấy cấu trúc xốp, độ ổn định sinh lý và cơ học và khả năng tương thích sinh học. Các nghiên cứu nuôi cấy tế bào trong ống nghiệm cho thấy nguyên bào sợi có thể phát triển, bám dính và lan rộng bên trong hydrogel, chỉ ra rằng hydrogel có thể hỗ trợ sửa chữa mô. Hơn nữa, hydrogel có thể hữu ích cho việc phân phối các phân tử sinh học. Yếu tố tăng trưởng nội mô mạch máu được bao bọc trong hydrogel và sau đó được giải phóng, giúp đẩy nhanh quá trình di chuyển của các tế bào nội mô tĩnh mạch rốn của người và tạo điều kiện thuận lợi cho quá trình chữa lành vết thương trong ống nghiệm. Nhìn chung, kết quả cho thấy hydrogel có thể là một phương tiện phân phối tiêm tiềm năng để chữa lành vết thương và sửa chữa mô."} +{"idx": 1871191, "en": "The flathead fish Platycephalus sp.1 is an ecologically and commercially important marine fish in the northwestern Pacific with notable sexual differences in growth and development. Yet the genomic data of this species is lacking. In the present study, whole genome sequencing of two individuals (one male and one female) of Platycephalus sp.1 were conducted to provide fundamental genomic information. The genome sizes were estimated to be 674.96 Mb (male) and 684.15 Mb (female) by using k-mer analyses. The heterozygosity and repeat ratios suggested possible male heterogamety of this species. The draft genome sequences were initially assembled and genome-wide microsatellite motifs were identified. Besides, the complete mitochondrial genome sequences were assembled and the phylogenetic analyses genetically supported the validation of Platycephalus sp.1. The reported genomic data and genetic markers in this study could be useful in future comparative genomics and evolutionary biology studies.", "gemini": "Loài cá bẹt Platycephalus sp.1 là một loài cá biển quan trọng về mặt sinh thái và thương mại ở vùng Tây Bắc Thái Bình Dương với sự khác biệt đáng kể về giới tính trong quá trình tăng trưởng và phát triển. Tuy nhiên, dữ liệu về bộ gen của loài này vẫn còn thiếu. Trong nghiên cứu hiện tại, trình tự toàn bộ bộ gen của hai cá thể (một đực và một cái) của Platycephalus sp.1 đã được tiến hành để cung cấp thông tin cơ bản về bộ gen. Kích thước bộ gen được ước tính là 674,96 Mb (con đực) và 684,15 Mb (con cái) bằng cách sử dụng phân tích k-mer. Tỷ lệ dị hợp tử và lặp lại cho thấy khả năng dị hợp tử ở con đực của loài này. Trình tự bản nháp bộ gen ban đầu đã được lắp ráp và các mô típ vi vệ tinh trên toàn bộ bộ gen đã được xác định. Bên cạnh đó, trình tự bộ gen ti thể hoàn chỉnh đã được lắp ráp và các phân tích phát sinh loài đã hỗ trợ về mặt di truyền cho việc xác nhận Platycephalus sp.1. Dữ liệu về bộ gen và các dấu hiệu di truyền được báo cáo trong nghiên cứu này có thể hữu ích trong các nghiên cứu về di truyền học so sánh và sinh học tiến hóa trong tương lai."} +{"idx": 1871192, "en": "The eutherian interferons were implicated as paradigmatic effector proteins in innate and acquired immunity. Yet, familiar interferon classification and nomenclature disagreed with functional genomics, phylogenetic and evolutionary analyses. Using eutherian comparative genomic analysis protocol and 35 public eutherian reference genomic sequence data sets, the present analysis attempted to resolve major disagreements in descriptions of eutherian interferons. Among 836 eutherian interferon potential coding sequences, the tests of reliability of eutherian public genomic sequences annotated, in aggregate, 495 complete coding sequences that were partitioned into supercluster IF1 and supercluster IF2 gene data sets. There were 29 human class 2 cytokine genes described, including initially described human IF1IC2 gene. There was convergence between nonhomologous eutherian supercluster IF1 and supercluster IF2 protein primary structures including common cysteine amino acid residues implicated in disulfide bonding. The integrated gene annotations, phylogenetic analysis and protein molecular analysis proposed revised and updated classification and nomenclature of eutherian interferons.", "gemini": "Các interferon ở thú có nhau thai được coi là các protein hiệu ứng mẫu mực trong khả năng miễn dịch bẩm sinh và mắc phải. Tuy nhiên, phân loại và danh pháp interferon quen thuộc lại không phù hợp với các phân tích về di truyền học chức năng, phát sinh loài và tiến hóa. Sử dụng giao thức phân tích di truyền học so sánh ở thú có nhau thai và 35 bộ dữ liệu trình tự bộ gen tham chiếu của thú có nhau thai công khai, phân tích hiện tại đã cố gắng giải quyết những bất đồng chính trong mô tả về các interferon ở thú có nhau thai. Trong số 836 trình tự mã hóa tiềm năng của interferon ở thú có nhau thai, các thử nghiệm về độ tin cậy của trình tự bộ gen công khai của thú có nhau thai được chú thích, tổng hợp 495 trình tự mã hóa hoàn chỉnh được phân thành các bộ dữ liệu gen siêu cụm IF1 và siêu cụm IF2. Có 29 gen cytokine lớp 2 của người được mô tả, bao gồm cả gen IF1IC2 của người được mô tả ban đầu. Có sự hội tụ giữa các cấu trúc chính của protein siêu cụm IF1 và siêu cụm IF2 không đồng dạng ở thú có nhau thai bao gồm các gốc axit amin cysteine phổ biến liên quan đến liên kết disulfide. Các chú thích gen tích hợp, phân tích phát sinh loài và phân tích phân tử protein đã đề xuất phân loại và danh pháp được sửa đổi và cập nhật của các interferon ở thú có nhau thai."} +{"idx": 1871193, "en": "Human papillomaviruses (HPVs) belongs to the Papillomaviridae family, which is divided into high-risk (HR), and low-risk (LR) HPVs based on their disease-causing competence. HR-HPVs 16 and 18 are known to cause distinct carcinomas like cervical and head and neck, whereas LR-HPVs are commonly associated with the genital warts. We have developed an integrative platform; HPVomics dedicated to the potential therapeutic regimens targeting all HPV genes including oncoproteins E6, E7 and E5. We primarily focused on eighteen HR-HPVs and eleven LR-HPVs. It mainly deals with therapeutically imperative elements, i.e., vaccine epitopes, siRNAs, sgRNAs, and anti-viral peptides. Simultaneously, it also comprises of genome browser, whole-genome sequences and annotation of HPVs with searching and filtering capabilities. Moreover, we have also developed an integrated support vector machine (SVM) based computational algorithm \"HPVepi\" for the prediction of HPV epitome. We hope that HPVomics (http://bioinfo.imtech.res.in/manojk/hpvomics/) will assist the scientific community engaged in HPV research.", "gemini": "Virus papilloma ở người (HPV) thuộc họ Papillomaviridae, được chia thành HPV nguy cơ cao (HR) và HPV nguy cơ thấp (LR) dựa trên khả năng gây bệnh của chúng. HPV 16 và 18 nguy cơ cao được biết là gây ra các bệnh ung thư riêng biệt như ung thư cổ tử cung và đầu cổ, trong khi HPV nguy cơ thấp thường liên quan đến mụn cóc sinh dục. Chúng tôi đã phát triển một nền tảng tích hợp; HPVomics dành riêng cho các chế độ điều trị tiềm năng nhắm vào tất cả các gen HPV bao gồm các oncoprotein E6, E7 và E5. Chúng tôi chủ yếu tập trung vào mười tám loại HPV nguy cơ cao và mười một loại HPV nguy cơ thấp. Nó chủ yếu liên quan đến các yếu tố bắt buộc trong điều trị, tức là epitop vắc-xin, siRNA, sgRNA và peptit chống vi-rút. Đồng thời, nó cũng bao gồm trình duyệt bộ gen, trình tự toàn bộ bộ gen và chú thích của HPV với khả năng tìm kiếm và lọc. Hơn nữa, chúng tôi cũng đã phát triển một thuật toán tính toán dựa trên máy vectơ hỗ trợ tích hợp (SVM) \"HPVepi\" để dự đoán biểu hiện HPV. Chúng tôi hy vọng rằng HPVomics (http://bioinfo.imtech.res.in/manojk/hpvomics/) sẽ hỗ trợ cộng đồng khoa học tham gia vào nghiên cứu về HPV."} +{"idx": 1871194, "en": "Insulin is a key hormone for maintaining glucose homeostasis in organisms. In general, deficiency of insulin synthesis and secretion results in type I diabetes, whereas insulin resistance leads to type 2 diabetes. Cell division cycle 42 (CDC42), a member of Rho GTPases family, has been shown as an essential regulator in the second phase of glucose-induced insulin secretion in pancreatic islets β cells in vitro. However, the effect of CDC42 on insulin expression has not been explored. Here we reported that the glucose-induced insulin expression and secretion were significantly inhibited in mice lacking CDC42 gene in pancreatic β cells (Rip-CDC42cKO) in vivo and in vitro. Deletion of CDC42 gene in pancreatic β cells did not affect survival or reproduction in mice. However, the Rip-CDC42cKO mice showed the systemic glucose intolerance and the decrease of glucose-induced insulin secretion without apparent alterations of peripheral tissues insulin sensitivity and the morphology of islets. Furthermore, we demonstrated that deletion of CDC42 gene in pancreatic β cells significantly attenuated the insulin expression through inhibiting the ERK1/2-NeuroD1 signaling pathway. Taken together, our study presents novel evidence that CDC42 is an important modulator in glucose-induced insulin expression as well as insulin secretion in pancreatic β cells.", "gemini": "Insulin là một loại hormone chính để duy trì cân bằng glucose trong các cơ quan. Nhìn chung, sự thiếu hụt tổng hợp và tiết insulin dẫn đến bệnh tiểu đường loại I, trong khi tình trạng kháng insulin dẫn đến bệnh tiểu đường loại 2. Chu kỳ phân chia tế bào 42 (CDC42), một thành viên của họ Rho GTPase, đã được chứng minh là một chất điều hòa thiết yếu trong giai đoạn thứ hai của quá trình tiết insulin do glucose kích thích trong các tế bào β đảo tụy trong ống nghiệm. Tuy nhiên, tác dụng của CDC42 đối với biểu hiện insulin vẫn chưa được khám phá. Ở đây chúng tôi báo cáo rằng biểu hiện và tiết insulin do glucose kích thích bị ức chế đáng kể ở những con chuột thiếu gen CDC42 trong các tế bào β tụy (Rip-CDC42cKO) trong ống nghiệm và trong cơ thể sống. Việc xóa gen CDC42 trong các tế bào β tụy không ảnh hưởng đến sự sống còn hoặc sinh sản ở chuột. Tuy nhiên, những con chuột Rip-CDC42cKO biểu hiện tình trạng không dung nạp glucose toàn thân và giảm tiết insulin do glucose kích thích mà không có sự thay đổi rõ ràng về độ nhạy insulin của các mô ngoại vi và hình thái của các đảo tụy. Hơn nữa, chúng tôi chứng minh rằng việc xóa gen CDC42 trong các tế bào β tụy làm giảm đáng kể biểu hiện insulin thông qua việc ức chế con đường tín hiệu ERK1/2-NeuroD1. Tóm lại, nghiên cứu của chúng tôi đưa ra bằng chứng mới rằng CDC42 là một chất điều biến quan trọng trong biểu hiện insulin do glucose kích thích cũng như tiết insulin trong các tế bào β tụy."} +{"idx": 1871195, "en": "Glucocorticoids (Gcs) potently inhibit inflammation, and regulate liver energy metabolism, often acting in a hypoxic environment. We now show hypoxic conditions open a specific GR cistrome, and prevent access of GR to part of the normoxic GR cistrome. Motif analysis identified enrichment of KLF4 binding sites beneath those peaks of GR binding exclusive to normoxia, implicating KLF4 as a pioneer, or co-factor under these conditions. Hypoxia reduced KLF4 expression, however, knockdown of KLF4 did not impair GR recruitment. KLF4 is a known target of microRNAs 103 and 107, both of which are induced by hypoxia. Expression of mimics to either microRNA103, or microRNA107 inhibited GR transactivation of normoxic target genes, thereby replicating the hypoxic effect. Therefore, studies in hypoxia reveal that microRNAs 103 and 107 are potent regulators of GR function. We have now identified a new pathway linking hypoxia through microRNAs 103 and 107 to regulation of GR function.", "gemini": "Glucocorticoid (Gc) ức chế mạnh tình trạng viêm và điều chỉnh quá trình chuyển hóa năng lượng của gan, thường hoạt động trong môi trường thiếu oxy. Hiện chúng tôi cho thấy tình trạng thiếu oxy mở ra một cistrome GR cụ thể và ngăn không cho GR tiếp cận một phần cistrome GR bình thường. Phân tích mô típ xác định sự làm giàu các vị trí liên kết KLF4 bên dưới các đỉnh liên kết GR chỉ dành riêng cho tình trạng bình thường, cho thấy KLF4 là chất tiên phong hoặc đồng yếu tố trong những điều kiện này. Tình trạng thiếu oxy làm giảm biểu hiện KLF4, tuy nhiên, việc hạ gục KLF4 không làm suy yếu khả năng tuyển dụng GR. KLF4 là mục tiêu đã biết của microRNA 103 và 107, cả hai đều được tạo ra bởi tình trạng thiếu oxy. Việc biểu hiện các bản sao giống với microRNA103 hoặc microRNA107 ức chế sự hoạt hóa gen mục tiêu bình thường của GR, do đó sao chép hiệu ứng thiếu oxy. Do đó, các nghiên cứu trong tình trạng thiếu oxy cho thấy microRNA 103 và 107 là những chất điều hòa mạnh mẽ chức năng GR. Hiện chúng tôi đã xác định được một con đường mới liên kết tình trạng thiếu oxy thông qua microRNA 103 và 107 để điều chỉnh chức năng GR."} +{"idx": 1871196, "en": "Two independent lines of evidence suggest that drowsiness and mind-wandering share common neurocognitive processes indexed by ocular parameters (e.g., eyeblink frequency and pupil dynamics). Mind-wandering and drowsiness frequently co-occur, however, such that it remains unclear whether observed oculometric variations are related to mind-wandering, drowsiness, or a mix of both. To address this issue, we assessed fluctuations in mind-wandering and sleepiness during a sustained attention task while ocular parameters were recorded. Results showed that oculometric variations during mind-wandering were fully explained by increased sleepiness. However, mind-wandering and sleepiness had additive deleterious effects on performance that were not fully explained by ocular parameters. These findings suggest that oculometric variations during task performance reflect increased drowsiness rather than processes specifically involved in mind-wandering, and that the neurocognitive processes indexed by oculometric parameters (e.g., regulatory processes of the locus coeruleus norepinephrine system) do not fully explain how mind-wandering and sleepiness cause attentional lapses.", "gemini": "Hai dòng bằng chứng độc lập cho thấy tình trạng buồn ngủ và sự lang thang của tâm trí có chung các quá trình nhận thức thần kinh được lập chỉ mục bằng các thông số về mắt (ví dụ: tần suất chớp mắt và động lực của đồng tử). Tuy nhiên, tình trạng lang thang của tâm trí và buồn ngủ thường xảy ra đồng thời, do đó vẫn chưa rõ liệu các biến thể về mắt quan sát được có liên quan đến tình trạng lang thang của tâm trí, buồn ngủ hay sự kết hợp của cả hai hay không. Để giải quyết vấn đề này, chúng tôi đã đánh giá những biến động trong tình trạng lang thang của tâm trí và buồn ngủ trong quá trình thực hiện nhiệm vụ chú ý liên tục trong khi ghi lại các thông số về mắt. Kết quả cho thấy các biến thể về mắt trong quá trình lang thang của tâm trí được giải thích đầy đủ bởi tình trạng buồn ngủ gia tăng. Tuy nhiên, tình trạng lang thang của tâm trí và buồn ngủ có tác động có hại cộng gộp đến hiệu suất, điều này không được giải thích đầy đủ bằng các thông số về mắt. Những phát hiện này cho thấy rằng các biến thể về mắt trong quá trình thực hiện nhiệm vụ phản ánh tình trạng buồn ngủ gia tăng chứ không phải các quá trình liên quan cụ thể đến tình trạng lang thang của tâm trí và các quá trình nhận thức thần kinh được lập chỉ mục bằng các thông số về mắt (ví dụ: các quá trình điều chỉnh của hệ thống norepinephrine locus coeruleus) không giải thích đầy đủ cách thức tình trạng lang thang của tâm trí và buồn ngủ gây ra tình trạng mất tập trung."} +{"idx": 1871197, "en": "Accurate discrimination between safe and dangerous stimuli is essential for survival. Prior research has begun to uncover the neural structures that are necessary for learning this discrimination, but exploration of brain regions involved in this learning process has been mostly limited to males. Recent findings show sex differences in discrimination learning, with reduced fear expression to safe cues in females compared to males. Here, we used male and female Sprague Dawley rats to explore neural activation, as measured by Fos expression, in fear and safety learning related brain regions. Neural activation after fear discrimination (Discrimination) was compared between males and females, as well as with fear conditioned (Fear Only) and stimulus presented (Control) conditions. Correlations of discrimination ability and neural activation were also calculated. We uncovered a correlation between central amygdala (CeA) activation and discrimination abilities in males and females. Anterior medial bed nucleus of the stria terminalis (BNST) was the only region where sex differences in Fos counts were observed in the Discrimination condition, and the only region where neural activation significantly differed between Fear Only and Discrimination conditions. Together, these findings indicate the importance of fear expression circuitry in mediating discrimination responses and generate important questions for future investigation.", "gemini": "Sự phân biệt chính xác giữa các kích thích an toàn và nguy hiểm là điều cần thiết để tồn tại. Các nghiên cứu trước đây đã bắt đầu khám phá các cấu trúc thần kinh cần thiết để học sự phân biệt này, nhưng việc khám phá các vùng não liên quan đến quá trình học này chủ yếu chỉ giới hạn ở nam giới. Những phát hiện gần đây cho thấy sự khác biệt về giới trong việc học phân biệt, với biểu hiện sợ hãi giảm đối với các tín hiệu an toàn ở nữ so với nam. Ở đây, chúng tôi đã sử dụng chuột Sprague Dawley đực và cái để khám phá sự hoạt hóa thần kinh, được đo bằng biểu hiện Fos, ở các vùng não liên quan đến việc học sợ hãi và an toàn. Sự hoạt hóa thần kinh sau khi phân biệt sợ hãi (Phân biệt) được so sánh giữa nam và nữ, cũng như với điều kiện sợ hãi có điều kiện (Chỉ sợ) và điều kiện kích thích được trình bày (Kiểm soát). Các mối tương quan về khả năng phân biệt và hoạt hóa thần kinh cũng được tính toán. Chúng tôi phát hiện ra mối tương quan giữa sự hoạt hóa hạnh nhân trung tâm (CeA) và khả năng phân biệt ở cả nam và nữ. Hạt nhân giường trước trung gian của rãnh tận cùng (BNST) là vùng duy nhất có sự khác biệt về giới trong số lượng Fos được quan sát thấy trong điều kiện Phân biệt và là vùng duy nhất mà sự hoạt hóa thần kinh khác biệt đáng kể giữa các điều kiện Chỉ sợ và Phân biệt. Cùng nhau, những phát hiện này chỉ ra tầm quan trọng của mạch biểu hiện sợ hãi trong việc điều hòa các phản ứng phân biệt và tạo ra những câu hỏi quan trọng cho nghiên cứu trong tương lai."} +{"idx": 1871198, "en": "Chronic metabolic health diseases are increasing worldwide placing strain on healthcare systems and importantly, impacting individuals' quality of life. It is well established that many chronic diseases are associated with inflammation and oxidative stress. Exercise is a known strategy to manage and treat inflammation in animals and humans. Understanding the mechanisms which cause acute and chronic changes to systems via various exercise protocols may provide insights into how we can better clinically manage patients with inflammatory and oxidative stress associated diseases. Nrf2 is a basic leucine transcription factor which regulates the expression of antioxidant proteins to protect against damage caused by electrophilic or oxidative stress. The aim of this narrative review is to provide an overview of the literature which has investigated the relationship between acute and chronic exercise training and Nrf2 protein, mRNA and Nrf2-ARE binding activity. This narrative review presents analysis of twenty-nine articles presenting studies using animals and humans. Findings from animal models suggest that exercise increases all molecular aspects of the Nrf2-ARE pathway in all tissues studied. It was noted that there seems to be an age-related decline in Nrf2 protein upregulation with exercise training. In humans, however, there is a lack of evidence to support this claim.", "gemini": "Các bệnh về sức khỏe chuyển hóa mãn tính đang gia tăng trên toàn thế giới gây căng thẳng cho hệ thống chăm sóc sức khỏe và quan trọng hơn là ảnh hưởng đến chất lượng cuộc sống của các cá nhân. Người ta đã biết rằng nhiều bệnh mãn tính liên quan đến tình trạng viêm và stress oxy hóa. Tập thể dục là một chiến lược đã biết để kiểm soát và điều trị tình trạng viêm ở động vật và con người. Hiểu được các cơ chế gây ra những thay đổi cấp tính và mãn tính đối với các hệ thống thông qua các phác đồ tập thể dục khác nhau có thể cung cấp thông tin chi tiết về cách chúng ta có thể quản lý tốt hơn bệnh nhân mắc các bệnh liên quan đến stress oxy hóa và viêm. Nrf2 là một yếu tố phiên mã leucine cơ bản điều chỉnh biểu hiện của các protein chống oxy hóa để bảo vệ chống lại tổn thương do stress oxy hóa hoặc ưa điện tử gây ra. Mục đích của bài tổng quan tường thuật này là cung cấp một cái nhìn tổng quan về các tài liệu đã nghiên cứu mối quan hệ giữa luyện tập thể dục cấp tính và mãn tính với protein Nrf2, mRNA và hoạt động liên kết Nrf2-ARE. Bài tổng quan tường thuật này trình bày phân tích của hai mươi chín bài báo trình bày các nghiên cứu sử dụng động vật và con người. Những phát hiện từ các mô hình động vật cho thấy rằng tập thể dục làm tăng tất cả các khía cạnh phân tử của con đường Nrf2-ARE ở tất cả các mô được nghiên cứu. Người ta thấy rằng có vẻ như có sự suy giảm liên quan đến tuổi trong việc điều hòa tăng protein Nrf2 với việc luyện tập thể dục. Tuy nhiên, ở người, vẫn thiếu bằng chứng để hỗ trợ tuyên bố này."} +{"idx": 1871199, "en": "Natural antioxidants, like phenolic acids, possess a unique chemical space that can protect cellular components from oxidative stress. However, their polar carboxylic acid chemotype reduces full intracellular antioxidant potential due to limited diffusion through biological membranes. Here, we have designed and developed a new generation of hydrophobic turn-on fluorescent antioxidant precursors that upon penetration of the cell membrane, reveal a more polar and more potent antioxidant core and simultaneously become fluorescent allowing their intracellular tracking. Their activation is stimulated by polarity alteration by sensing intracellular signals and specifically biothiols. In our design, the carboxylic group of phenolic acids that originally restricts cell entrance is derivatized and conjugated through Copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) to a coumarin derivative that its fluorescence properties are quenched with a biothiol activatable element. This more hydrophobic precursor readily penetrates cell membrane and once inside the cell the antioxidant core is revealed upon sensing glutathione, its fluorescence is restored in a turn-on manner and the generation of a more polar character traps the molecule inside the cell. This turn-on fluorescent antioxidant precursor that can be applied to phenolic acids, was developed for rosmarinic acid and the conjugate was named as RCG. The selectivity and responsiveness of RCG towards the most abundant biothiols was monitored through a variety of biophysical techniques including UV-Vis, fluorescence and NMR spectroscopy. The electrochemical behavior and free radical scavenging capacity of the precursor RCG and the active compound (RC) was evaluated and compared with the parent compound (rosmarinic acid) through cyclic voltammetry and EPR spectroscopy, respectively. The stability of the newly synthesized bioactive conjugate RC was found significantly higher than the parent rosmarinic acid when exposed to oxygen. Cell uptake experiments were conducted and revealed the internalization of RCG. The degree of intracellular DNA protection offered by RCG and its active drug (RC) on exposure to H2O2 was also evaluated in Jurkat cells.", "gemini": "Các chất chống oxy hóa tự nhiên, như axit phenolic, sở hữu một không gian hóa học độc đáo có thể bảo vệ các thành phần tế bào khỏi stress oxy hóa. Tuy nhiên, kiểu hình axit cacboxylic phân cực của chúng làm giảm tiềm năng chống oxy hóa nội bào hoàn toàn do sự khuếch tán hạn chế qua màng sinh học. Tại đây, chúng tôi đã thiết kế và phát triển một thế hệ mới các tiền chất chống oxy hóa huỳnh quang bật tắt kỵ nước mà khi thâm nhập vào màng tế bào, sẽ bộc lộ một lõi chống oxy hóa phân cực hơn và mạnh hơn đồng thời trở nên huỳnh quang cho phép theo dõi chúng trong tế bào. Sự hoạt hóa của chúng được kích thích bởi sự thay đổi cực tính bằng cách cảm nhận các tín hiệu nội bào và đặc biệt là các sinh thiol. Trong thiết kế của chúng tôi, nhóm cacboxylic của axit phenolic ban đầu hạn chế sự xâm nhập vào tế bào được dẫn xuất và liên hợp thông qua phản ứng cộng vòng azide-ankin xúc tác bởi đồng (I) (CuAAC) với dẫn xuất coumarin có đặc tính huỳnh quang bị dập tắt với một yếu tố hoạt hóa sinh thiol. Tiền chất kỵ nước hơn này dễ dàng thâm nhập vào màng tế bào và khi vào bên trong tế bào, lõi chống oxy hóa được bộc lộ khi cảm nhận được glutathione, huỳnh quang của nó được phục hồi theo cách bật và sự tạo ra một đặc tính phân cực hơn sẽ giữ phân tử bên trong tế bào. Tiền chất chống oxy hóa huỳnh quang bật tắt này có thể được áp dụng cho axit phenolic, được phát triển cho axit rosmarinic và hợp chất liên hợp được đặt tên là RCG. Độ chọn lọc và khả năng phản ứng của RCG đối với các sinh thiol dồi dào nhất được theo dõi thông qua nhiều kỹ thuật sinh lý học bao gồm quang phổ UV-Vis, huỳnh quang và NMR. Hành vi điện hóa và khả năng dọn gốc tự do của tiền chất RCG và hợp chất hoạt tính (RC) đã được đánh giá và so sánh với hợp chất gốc (axit rosmarinic) thông qua phép đo voltam vòng và phép đo quang phổ EPR. Độ ổn định của hợp chất liên hợp sinh học hoạt tính RC mới được tổng hợp được phát hiện cao hơn đáng kể so với axit rosmarinic gốc khi tiếp xúc với oxy. Các thí nghiệm hấp thu tế bào đã được tiến hành và cho thấy sự nội tại hóa của RCG. Mức độ bảo vệ DNA nội bào do RCG và thuốc hoạt tính của nó (RC) cung cấp khi tiếp xúc với H2O2 cũng được đánh giá trong các tế bào Jurkat."} diff --git a/retranslated_vi_pubmed/chunk_187/error.json b/retranslated_vi_pubmed/chunk_187/error.json new file mode 100644 index 0000000000000000000000000000000000000000..84b889adfc9dfad0ea9665f16862b7f0ec24fb29 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_187/error.json @@ -0,0 +1,5 @@ +[ + 1870723, + 1870724, + 1870725 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_188/completed.json b/retranslated_vi_pubmed/chunk_188/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..fd7c5364b76cafcdf1dec798e7b0c9b9a40601d7 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_188/completed.json @@ -0,0 +1,146 @@ +[ + 1880000, + 1880001, + 1880002, + 1880003, + 1880004, + 1880005, + 1880006, + 1880007, + 1880008, + 1880009, + 1880010, + 1880011, + 1880012, + 1880013, + 1880014, + 1880015, + 1880016, + 1880017, + 1880018, + 1880019, + 1880020, + 1880021, + 1880022, + 1880023, + 1880024, + 1880025, + 1880026, + 1880027, + 1880028, + 1880029, + 1880030, + 1880031, + 1880032, + 1880033, + 1880034, + 1880035, + 1880036, + 1880037, + 1880038, + 1880039, + 1880040, + 1880041, + 1880042, + 1880043, + 1880044, + 1880045, + 1880046, + 1880047, + 1880048, + 1880049, + 1880050, + 1880051, + 1880052, + 1880053, + 1880054, + 1880055, + 1880056, + 1880057, + 1880058, + 1880059, + 1880060, + 1880061, + 1880062, + 1880063, + 1880064, + 1880065, + 1880066, + 1880067, + 1880068, + 1880069, + 1880070, + 1880071, + 1880072, + 1880073, + 1880074, + 1880075, + 1880076, + 1880077, + 1880078, + 1880079, + 1880080, + 1880081, + 1880082, + 1880083, + 1880084, + 1880085, + 1880086, + 1880087, + 1880088, + 1880089, + 1880090, + 1880091, + 1880092, + 1880093, + 1880094, + 1880095, + 1880096, + 1880097, + 1880098, + 1880099, + 1880100, + 1880101, + 1880102, + 1880103, + 1880104, + 1880105, + 1880106, + 1880107, + 1880108, + 1880109, + 1880110, + 1880111, + 1880112, + 1880113, + 1880114, + 1880115, + 1880116, + 1880117, + 1880118, + 1880119, + 1880120, + 1880121, + 1880122, + 1880123, + 1880124, + 1880125, + 1880126, + 1880127, + 1880128, + 1880129, + 1880130, + 1880131, + 1880132, + 1880133, + 1880134, + 1880135, + 1880136, + 1880137, + 1880138, + 1880139, + 1880140, + 1880141, + 1880142, + 1880143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_188/data.jsonl b/retranslated_vi_pubmed/chunk_188/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..8c4fba392395ba5376d49ce275801974ff0384a7 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_188/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1880000, "en": "Ultrawideline 35Cl solid-state nuclear magnetic resonance (SSNMR) spectra of a series of 12 tin chlorides were recorded. The magnitude of the 35Cl quadrupolar coupling constant (CQ) was shown to consistently indicate the chemical state (oxidation number) of the bound Sn center. The chemical state of the Sn center was independently verified by tin Mössbauer spectroscopy. CQ(35Cl) values of >30 MHz correspond to Sn(IV), while CQ(35Cl) readings of <30 MHz indicate that Sn(II) is present. Tin-119 SSNMR experiments would seem to be the most direct and effective route to interrogating tin in these systems, yet we show that ambiguous results can emerge from this method, which may lead to an incorrect interpretation of the Sn oxidation number. The accumulated 35Cl NMR data are used as a guide to assign the Sn oxidation number in the mixed-valent metal complex Ph3PPdImSnCl2. The synthesis and crystal structure of the related Ph3PPtImSnCl2 are reported, and 195Pt and 35Cl SSNMR experiments were also used to investigate its Pt-Sn bonding. Plane-wave DFT calculations of 35Cl, 119Sn, and 195Pt NMR parameters are used to model and interpret experimental data, supported by computed 119Sn and 195Pt chemical shift tensor orientations. Given the ubiquity of directly bound Cl centers in organometallic and inorganic systems, there is tremendous potential for widespread usage of 35Cl SSNMR parameters to provide a reliable indication of the chemical state in metal chlorides.", "gemini": "Phổ cộng hưởng từ hạt nhân trạng thái rắn (SSNMR) 35Cl siêu rộng của một loạt 12 clorua thiếc đã được ghi lại. Độ lớn của hằng số ghép tứ cực 35Cl (CQ) được chứng minh là liên tục chỉ ra trạng thái hóa học (số oxy hóa) của tâm Sn liên kết. Trạng thái hóa học của tâm Sn được xác minh độc lập bằng phương pháp quang phổ Mössbauer thiếc. Giá trị CQ(35Cl) >30 MHz tương ứng với Sn(IV), trong khi giá trị CQ(35Cl) <30 MHz chỉ ra sự có mặt của Sn(II). Các thí nghiệm SSNMR Tin-119 có vẻ là con đường trực tiếp và hiệu quả nhất để thẩm vấn thiếc trong các hệ thống này, nhưng chúng tôi chỉ ra rằng các kết quả mơ hồ có thể xuất hiện từ phương pháp này, có thể dẫn đến việc giải thích sai về số oxy hóa Sn. Dữ liệu NMR 35Cl tích lũy được sử dụng như một hướng dẫn để chỉ định số oxy hóa Sn trong hợp chất phức kim loại có hóa trị hỗn hợp Ph3PPdImSnCl2. Tổng hợp và cấu trúc tinh thể của Ph3PPtImSnCl2 liên quan được báo cáo và các thí nghiệm SSNMR 195Pt và 35Cl cũng được sử dụng để điều tra liên kết Pt-Sn của nó. Các phép tính DFT sóng phẳng của các thông số NMR 35Cl, 119Sn và 195Pt được sử dụng để mô hình hóa và giải thích dữ liệu thực nghiệm, được hỗ trợ bởi các định hướng tenxơ dịch chuyển hóa học 119Sn và 195Pt được tính toán. Với sự phổ biến của các tâm Cl liên kết trực tiếp trong các hệ thống organometallic và vô cơ, có tiềm năng to lớn để sử dụng rộng rãi các thông số SSNMR 35Cl để cung cấp một chỉ báo đáng tin cậy về trạng thái hóa học trong các clorua kim loại."} +{"idx": 1880001, "en": "Novel 1-(1-benzoylpiperidin-4-yl)methanamine derivatives with high affinity and selectivity for serotonin 5-HT1A receptors were obtained and tested in four functional assays: ERK1/2 phosphorylation, adenylyl cyclase inhibition, calcium mobilization, and β-arrestin recruitment. Compounds 44 and 56 (2-methylaminophenoxyethyl and 2-(1H-indol-4-yloxy)ethyl derivatives, respectively) were selected as biased agonists with highly differential \"signaling fingerprints\" that translated into distinct in vivo profiles. In vitro, 44 showed biased agonism for ERK1/2 phosphorylation and, in vivo, it preferentially exerted an antidepressant-like effect in the Porsolt forced swimming test in rats. In contrast, compound 56 exhibited a first-in-class profile: it preferentially and potently activated β-arrestin recruitment in vitro and potently elicited lower lip retraction in vivo, a component of \"serotonergic syndrome\". Both compounds showed promising developability properties. The presented 5-HT1A receptor-biased agonists, preferentially targeting various signaling pathways, have the potential to become drug candidates for distinct central nervous system pathologies and possessing accentuated therapeutic activity and reduced side effects.", "gemini": "Các dẫn xuất 1-(1-benzoylpiperidin-4-yl)methanamine mới có ái lực và tính chọn lọc cao đối với thụ thể serotonin 5-HT1A đã được thu được và thử nghiệm trong bốn xét nghiệm chức năng: phosphoryl hóa ERK1/2, ức chế adenyl cyclase, huy động canxi và tuyển dụng β-arrestin. Các hợp chất 44 và 56 (tương ứng là dẫn xuất 2-methylaminophenoxyethyl và 2-(1H-indol-4-yloxy)ethyl) được chọn làm chất chủ vận có thiên hướng với \"dấu vân tay tín hiệu\" khác biệt cao được dịch thành các hồ sơ in vivo riêng biệt. Trong ống nghiệm, 44 cho thấy sự chủ vận có thiên hướng đối với sự phosphoryl hóa ERK1/2 và trong ống nghiệm, nó chủ yếu gây ra tác dụng giống như thuốc chống trầm cảm trong thử nghiệm bơi cưỡng bức Porsolt ở chuột. Ngược lại, hợp chất 56 biểu hiện một hồ sơ đầu tiên trong lớp: nó chủ yếu và mạnh mẽ kích hoạt sự tuyển dụng β-arrestin trong ống nghiệm và mạnh mẽ gây ra co rút môi dưới trong ống nghiệm, một thành phần của \"hội chứng serotonergic\". Cả hai hợp chất đều cho thấy các đặc tính phát triển đầy hứa hẹn. Các chất chủ vận có thiên hướng thụ thể 5-HT1A được trình bày, chủ yếu nhắm vào các con đường truyền tín hiệu khác nhau, có khả năng trở thành ứng cử viên thuốc cho các bệnh lý hệ thần kinh trung ương riêng biệt và sở hữu hoạt tính điều trị cao hơn và giảm tác dụng phụ."} +{"idx": 1880002, "en": "In designing organic semiconductors for organic devices, halogenation is a very popular strategy for tuning the electronic properties and packing arrangement in the solid state. Herein, we report the synthesis and characterization of halogenated dibenzo[a,j]perylene (DBP) with triethylsilyl (TES)-ethynyl substituents at the 8- and 16-positions (TES-DBP). The resulting compounds are characterized by optical, electrochemical, crystallographic, and computational studies to clarify the effect of halogenation on the optoelectronic properties and charge-carrier transport. It is found that the halogen atoms, the degree of halogenation, and their positional locations can alter the electronic properties and crystal packing of the compounds. In contrast to fluorinated TES-DBP, the chlorinated counterpart has red-shifted maximum absorption and lower electron affinity owing to the electron delocalization between DBP core and the unoccupied 3d orbitals of Cl atom. Organic field-effect transistor measurements demonstrate that TES-2ClDBP shows a hole mobility of 0.25 cm2 V-1 s-1, which is higher than TES-2FDBP and TES-DBP. On the other hand, TES-4ClDBP exhibits ambipolar transport characteristics with electron and hole mobilities up to 0.02 and 0.07 cm2 V-1 s-1, respectively.", "gemini": "Trong thiết kế chất bán dẫn hữu cơ cho các thiết bị hữu cơ, halogen hóa là một chiến lược rất phổ biến để điều chỉnh các tính chất điện tử và sự sắp xếp đóng gói trong trạng thái rắn. Ở đây, chúng tôi báo cáo về tổng hợp và đặc tính của dibenzo[a,j]perylene (DBP) halogen hóa với các nhóm thế triethylsilyl (TES) -ethynyl ở vị trí 8 và 16 (TES-DBP). Các hợp chất thu được được đặc trưng bằng các nghiên cứu quang học, điện hóa, tinh thể học và tính toán để làm rõ tác động của halogen hóa đối với các tính chất quang điện tử và quá trình vận chuyển điện tích. Người ta thấy rằng các nguyên tử halogen, mức độ halogen hóa và vị trí của chúng có thể làm thay đổi các tính chất điện tử và sự đóng gói tinh thể của các hợp chất. Trái ngược với TES-DBP được fluorin hóa, chất tương ứng được clo hóa có khả năng hấp thụ cực đại dịch chuyển về phía đỏ và ái lực điện tử thấp hơn do sự dịch chuyển điện tử giữa lõi DBP và các quỹ đạo 3d chưa chiếm chỗ của nguyên tử Cl. Các phép đo bóng bán dẫn hiệu ứng trường hữu cơ chứng minh rằng TES-2ClDBP cho thấy độ linh động lỗ là 0,25 cm2 V-1 s-1, cao hơn TES-2FDBP và TES-DBP. Mặt khác, TES-4ClDBP biểu hiện các đặc tính vận chuyển lưỡng cực với độ linh động của electron và lỗ lần lượt lên tới 0,02 và 0,07 cm2 V-1 s-1."} +{"idx": 1880003, "en": "Spreading or pinning of a liquid drop on a solid substrate is determined by the surface energy of solid and liquid, topography of substrate surface, and different external forces like electric field, magnetic field, and vibration. Here we present a novel mechanism of depinning, driven by in situ generation of a species following reaction between a constituent of the droplet and one in the substrate. In particular, fluoro-carbon (FC) functionalized agarose and pHEMA gels are used as the substrates; the substrate is soaked with chloroauric acid. A drop of poly(dimethylsiloxane) (PDMS) mixed with the cross-linking agent is dispensed on it. The drop does not spread in absence of the salt, but as the salt concentration increases, the spreading diameter increases with decrease in the contact angle. The Si-H group, present as a constituent in the cross-linking agent, reduces the salt, leading to in situ generation of gold nanoparticles, that mitigates the pinning effect of the drop and the drop spreads.", "gemini": "Việc lan rộng hoặc ghim một giọt chất lỏng trên một chất rắn được xác định bởi năng lượng bề mặt của chất rắn và chất lỏng, địa hình bề mặt của chất rắn và các lực bên ngoài khác nhau như trường điện, trường từ và rung động. Ở đây chúng tôi trình bày một cơ chế gỡ ghim mới, được thúc đẩy bởi sự tạo ra tại chỗ một loài sau phản ứng giữa một thành phần của giọt và một thành phần trong chất nền. Đặc biệt, agarose chức năng hóa fluoro-carbon (FC) và gel pHEMA được sử dụng làm chất nền; chất nền được ngâm trong axit chloroauric. Một giọt poly (dimethylsiloxane) (PDMS) được trộn với chất liên kết chéo được phân phối trên đó. Giọt không lan rộng khi không có muối, nhưng khi nồng độ muối tăng, đường kính lan rộng tăng cùng với sự giảm của góc tiếp xúc. Nhóm Si-H, có mặt như một thành phần trong chất liên kết chéo, làm giảm muối, dẫn đến sự tạo thành tại chỗ các hạt nano vàng, làm giảm hiệu ứng ghim của giọt và giọt lan rộng."} +{"idx": 1880004, "en": "Cu, Al, and 316L stainless steel are the main components of the current collectors and coin-type cells used in the characterization of potassium-ion battery (KIB) materials and are expected to be electrochemically inactive. Herein, their electrochemical stabilities in a nonaqueous potassium-bis(fluorosulfonyl)imide (KFSI)-based electrolyte are investigated. In dynamic- and transient-mode polarization, passivation of each metal is observed to occur below 3.9, 3.8, and 4.05 V versus K+/K for Cu, Al, and 316L stainless steel, respectively, which are considered the threshold potentials. The composition of the passive layers of each metal is determined using time-of-flight secondary-ion mass spectrometry. The passive layers of Cu and Al consist of Cu-O (CuO or Cu2O) and Al-O (Al2O3), respectively, and 316L stainless steel is passivated with an outermost Cr-F (CrF3) layer and an inner Cr-O (Cr2O3) layer. Above the threshold potentials, however, severe corrosion of each metal occurs accompanied by the dissolution of metal ions, which could affect the reliability of experimental results for KIBs using KFSI-based electrolytes.", "gemini": "Cu, Al và thép không gỉ 316L là các thành phần chính của bộ thu dòng điện và pin loại tiền xu được sử dụng trong quá trình xác định đặc tính vật liệu pin ion kali (KIB) và được dự kiến là không hoạt động điện hóa. Trong bài báo này, tính ổn định điện hóa của chúng trong chất điện phân dựa trên kali-bis (fluorosulfonyl) imide (KFSI) không chứa nước được nghiên cứu. Trong quá trình phân cực ở chế độ động và chế độ tạm thời, quá trình thụ động hóa của từng kim loại được quan sát thấy xảy ra dưới 3,9, 3,8 và 4,05 V so với K + / K đối với Cu, Al và thép không gỉ 316L, tương ứng, được coi là ngưỡng tiềm năng. Thành phần của các lớp thụ động của từng kim loại được xác định bằng phương pháp phổ khối ion thứ cấp thời gian bay. Các lớp thụ động của Cu và Al lần lượt bao gồm Cu-O (CuO hoặc Cu2O) và Al-O (Al2O3), và thép không gỉ 316L được thụ động hóa với lớp Cr-F (CrF3) ngoài cùng và lớp Cr-O (Cr2O3) bên trong. Tuy nhiên, trên ngưỡng tiềm năng, sự ăn mòn nghiêm trọng của từng kim loại xảy ra kèm theo sự hòa tan các ion kim loại, điều này có thể ảnh hưởng đến độ tin cậy của kết quả thử nghiệm đối với KIB sử dụng chất điện phân gốc KFSI."} +{"idx": 1880005, "en": "Density functional theory (DFT)-based methods often significantly underpredict chemical reaction barriers compared with experiments because of the tendency of DFT to overstabilize transition states with stretched bonds due to the impact of unphysical electron self-interaction. However, many reactions have early or late transition states where the transition state geometry closely resembles the reactants or products, respectively. The role of self-interaction in those cases is not known. Here we compare the performance of DFT with and without self-interaction correction (SIC) for describing the hydrogenation of CO and CO2 catalyzed by a Lewis acid-base pair incorporated onto an aromatic cluster, using CCSD(T) results for reference. The three elementary steps in these reactions consist of an early, a middle, and a late transition. Our results show that the Perdew-Zunger SIC (PZ-SIC), implemented in the Fermi-Löwdin orbital SIC (FLO-SIC) approach, qualitatively improves the description of the forward and reverse reaction barriers relative to uncorrected DFT for the middle transition but not the early or late transitions. By contrast, the local scaling SIC (LSIC) method, also implemented in the FLO-SIC framework, significantly improves the calculated barriers over DFT and PZ-SIC in all but one case. The results also show how the FLO-SIC approach can provide insight into the bonding in aromatic systems.", "gemini": "Các phương pháp dựa trên lý thuyết mật độ hàm (DFT) thường dự đoán thấp đáng kể các rào cản phản ứng hóa học so với các thí nghiệm vì xu hướng DFT làm ổn định quá mức các trạng thái chuyển tiếp có liên kết kéo dài do tác động của tương tác tự điện tử không vật lý. Tuy nhiên, nhiều phản ứng có trạng thái chuyển tiếp sớm hoặc muộn trong đó hình học trạng thái chuyển tiếp tương ứng chặt chẽ với chất phản ứng hoặc sản phẩm. Vai trò của tương tác tự trong những trường hợp đó vẫn chưa được biết rõ. Ở đây, chúng tôi so sánh hiệu suất của DFT có và không có hiệu chỉnh tương tác tự (SIC) để mô tả quá trình hydro hóa CO và CO2 được xúc tác bởi một cặp axit-bazơ Lewis kết hợp vào một cụm thơm, sử dụng kết quả CCSD(T) để tham khảo. Ba bước cơ bản trong các phản ứng này bao gồm một giai đoạn chuyển tiếp sớm, giữa và muộn. Kết quả của chúng tôi cho thấy Perdew-Zunger SIC (PZ-SIC), được triển khai theo phương pháp SIC quỹ đạo Fermi-Löwdin (FLO-SIC), cải thiện định tính mô tả của các rào cản phản ứng thuận nghịch so với DFT chưa hiệu chỉnh đối với giai đoạn chuyển tiếp giữa nhưng không phải là giai đoạn chuyển tiếp sớm hoặc muộn. Ngược lại, phương pháp SIC tỷ lệ cục bộ (LSIC), cũng được triển khai trong khuôn khổ FLO-SIC, cải thiện đáng kể các rào cản được tính toán trên DFT và PZ-SIC trong tất cả các trường hợp trừ một trường hợp. Kết quả cũng chỉ ra cách tiếp cận FLO-SIC có thể cung cấp cái nhìn sâu sắc về liên kết trong các hệ thơm."} +{"idx": 1880006, "en": "[2,1]Benzothiazine S,S-dioxides 2 were synthesized by simply heating o-nitrostyrenes with elemental sulfur in 3-picoline with complete atom economy. This reaction was found to occur without any added catalyst and consist of a cascade of reduction of the nitro group, sulfuration of a C-H of the double bond, oxidation of a sulfur atom to its highest oxidation state by the migration of two oxygen atoms from the nitro group, and formation of new N-S bonds. Furthermore, the method could also be applied to o-nitrocinnamamides and cinnamate esters.", "gemini": "[2,1]Benzothiazine S,S-dioxide 2 được tổng hợp bằng cách đơn giản là đun nóng o-nitrostyren với lưu huỳnh nguyên tố trong 3-picoline với hiệu suất nguyên tử hoàn toàn. Phản ứng này được phát hiện xảy ra mà không có bất kỳ chất xúc tác nào được thêm vào và bao gồm một loạt các phản ứng khử nhóm nitro, sunfua hóa C-H của liên kết đôi, oxy hóa một nguyên tử lưu huỳnh lên trạng thái oxy hóa cao nhất bằng cách di chuyển hai nguyên tử oxy từ nhóm nitro và hình thành liên kết N-S mới. Hơn nữa, phương pháp này cũng có thể được áp dụng cho o-nitrocinnamamide và este cinnamat."} +{"idx": 1880007, "en": "Present herein is a stepwise assembly method toward aluminum-lanthanide-based (Al-Ln) compounds. From the perspective of charge balance, polyanions are necessary to bind with Ln ions. However, the synthesis of polyanions aluminum compounds remains quite challenging. Herein, two Al4 polyanions [Al4(L)4(Cat)2]·4Hdma (AlOC-13, H3L= 2,3-dihydroxybenzoic acid, Cat = catechol, and dma = dimethylamine) and [Al4(L)4(HL)2(DMF)2]·4Hdma·0.5DMF·0.5H2O (AlOC-14, DMF = N,N-dimethylformamide) were successfully obtained under solvothermal conditions. Catechol and Hdma were generated from the in situ decarboxylation of H3L ligand and decomposition of DMF, respectively. AlOC-13 is qualified for further coordination assembly for the available vacancy coordination sites, good water solubility, and scale-up synthesis. The assembly of Al4 polyanions with equivalent Ln ions afforded a series of zigzag chain structures [LnAl4(L)4(Cat)2(DMF)2(H2O)3]·Hdma (Ln = Sm, Eu, Gd, Tb, Dy, Ho, Er, Yb) (AlOC-13-Ln). Moreover, the magnetic behavior and photoluminescence of the series of AlOC-13-Ln were also studied. AlOC-13-Dy shows obvious antiferromagnetic behavior, while AlOC-13-Tb exhibits excellent green characteristic luminescence. This study not only paves the way toward anionic aluminum clusters but also reveals their potential application in water treatment of cationic metal ions capture.", "gemini": "Có mặt ở đây là một phương pháp lắp ráp từng bước hướng tới các hợp chất dựa trên nhôm-lanthanide (Al-Ln). Theo quan điểm cân bằng điện tích, các polyanion là cần thiết để liên kết với các ion Ln. Tuy nhiên, tổng hợp các hợp chất nhôm polyanion vẫn còn khá nhiều thách thức. Ở đây, hai polyanion Al4 [Al4(L)4(Cat)2]·4Hdma (AlOC-13, H3L = axit 2,3-dihydroxybenzoic, Cat = catechol và dma = dimethylamine) và [Al4(L)4(HL)2(DMF)2]·4Hdma·0.5DMF·0.5H2O (AlOC-14, DMF = N,N-dimethylformamide) đã được tổng hợp thành công trong điều kiện dung môi nhiệt. Catechol và Hdma lần lượt được tạo ra từ quá trình decarboxyl hóa tại chỗ của phối tử H3L và sự phân hủy của DMF. AlOC-13 đủ điều kiện để lắp ráp phối hợp tiếp theo cho các vị trí phối hợp chỗ trống có sẵn, độ hòa tan trong nước tốt và tổng hợp theo quy mô. Việc lắp ráp các polyanion Al4 với các ion Ln tương đương tạo ra một loạt các cấu trúc chuỗi ngoằn ngoèo [LnAl4(L)4(Cat)2(DMF)2(H2O)3]·Hdma (Ln = Sm, Eu, Gd, Tb, Dy, Ho, Er, Yb) (AlOC-13-Ln). Hơn nữa, tính chất từ và phát quang của loạt AlOC-13-Ln cũng được nghiên cứu. AlOC-13-Dy cho thấy tính chất phản sắt từ rõ ràng, trong khi AlOC-13-Tb thể hiện phát quang đặc trưng màu xanh lá cây tuyệt vời. Nghiên cứu này không chỉ mở đường cho các cụm nhôm anion mà còn tiết lộ ứng dụng tiềm năng của chúng trong xử lý nước để thu giữ các ion kim loại cation."} +{"idx": 1880008, "en": "An efficient three-component domino or one-pot strategy has been developed for the synthesis of medicinally important benzothiophene and benzothiopheno[2,3-e]azepinedione derivatives for the first time. Amine-promoted selective cleavage of C-S bond of thioisatin is the key step in this process. The reported methodology benefits from environmentally friendly solvent (H2O), wide substrate scope, good functional group tolerance, and high reaction yields.", "gemini": "Một chiến lược domino ba thành phần hiệu quả hoặc một nồi đã được phát triển để tổng hợp các dẫn xuất benzothiophene và benzothiopheno[2,3-e]azepinedione quan trọng về mặt y học lần đầu tiên. Phản ứng cắt chọn lọc liên kết C-S của thioisatin xúc tác bằng amin là bước quan trọng trong quá trình này. Phương pháp đã báo cáo được hưởng lợi từ dung môi thân thiện với môi trường (H2O), phạm vi cơ chất rộng, khả năng chịu nhóm chức năng tốt và năng suất phản ứng cao."} +{"idx": 1880009, "en": "The conjugate addition reactions of trans-1,2-di(2-pyridyl)ethylene have been studied. This substrate reacts with organolithium nucleophiles, and the resulting anionic intermediates may be trapped by proton or various carbonyl-based electrophiles. It is suggested that the dipyridyl structure stabilizes the intermediate carbanion, allowing the Michael adduct to be captured by an added electrophile.", "gemini": "Phản ứng cộng liên hợp của trans-1,2-di(2-pyridyl)ethylene đã được nghiên cứu. Chất nền này phản ứng với các chất ái nhân organolithium và các chất trung gian anion thu được có thể bị giữ lại bởi proton hoặc các chất ái điện khác nhau có gốc cacbonyl. Người ta cho rằng cấu trúc dipyridyl ổn định carbanion trung gian, cho phép sản phẩm cộng Michael bị giữ lại bởi một chất ái điện được thêm vào."} +{"idx": 1880010, "en": "The COVID-19 pandemic rapidly became a worldwide healthcare emergency affecting millions of people, with poor outcomes for patients with chronic conditions and enormous pressure on healthcare systems. Pulmonary fibrosis (PF) has been cited as a risk factor for a more severe evolution of COVID-19, primarily because its acute exacerbations are already associated with high mortality. We reviewed the available literature on biochemical, pathophysiological, and pharmacological mechanisms of PF and COVID-19 in an attempt to foresee the particular risk of infection and possible evolution of PF patients if infected with SARS-COV-2. We also analyzed the possible role of medication and risk factors (such as smoking) in the disease's evolution and clinical course. We found out that there is a complexity of interactions between coexisting idiopathic pulmonary fibrosis/interstitial lung disease (ILD) and COVID-19 disease. Also, patients recovering from severe COVID-19 disease are at serious risk of developing PF. Smokers seem to have, in theory, a chance for a better outcome if they develop a severe form of COVID-19 but statistically are at much higher risk of dying if they become critically ill.", "gemini": "Đại dịch COVID-19 nhanh chóng trở thành tình trạng khẩn cấp về chăm sóc sức khỏe trên toàn thế giới, ảnh hưởng đến hàng triệu người, với kết cục không tốt đối với những bệnh nhân mắc các bệnh mãn tính và gây áp lực rất lớn lên các hệ thống chăm sóc sức khỏe. Xơ phổi (PF) được coi là yếu tố nguy cơ khiến COVID-19 diễn biến nghiêm trọng hơn, chủ yếu là do các đợt cấp tính của bệnh này vốn đã liên quan đến tỷ lệ tử vong cao. Chúng tôi đã xem xét các tài liệu hiện có về các cơ chế sinh hóa, bệnh sinh và dược lý của PF và COVID-19 để cố gắng dự đoán nguy cơ nhiễm trùng cụ thể và diễn biến có thể xảy ra của bệnh nhân PF nếu bị nhiễm SARS-COV-2. Chúng tôi cũng phân tích vai trò có thể có của thuốc và các yếu tố nguy cơ (như hút thuốc) trong quá trình diễn biến và diễn tiến lâm sàng của bệnh. Chúng tôi phát hiện ra rằng có sự phức tạp trong tương tác giữa bệnh xơ phổi tự phát/bệnh phổi kẽ (ILD) cùng tồn tại và bệnh COVID-19. Ngoài ra, những bệnh nhân hồi phục sau bệnh COVID-19 nghiêm trọng có nguy cơ mắc PF nghiêm trọng. Về mặt lý thuyết, những người hút thuốc dường như có cơ hội có kết cục tốt hơn nếu mắc COVID-19 dạng nghiêm trọng nhưng về mặt thống kê, họ có nguy cơ tử vong cao hơn nhiều nếu bị bệnh nặng."} +{"idx": 1880011, "en": "Mesoporous silica MCM-48 with rich silanol was prepared using polyvinylpyrrolidone (PVP) and cetyltrimethylammonium bromide (CTAB) as mixed templates, and the dynamic adsorption performance of acetone was evaluated by testing breakthrough curves. The mixed micelle formed by CTAB and PVP, as well as the hydrogen bond between the carbonyl group of PVP and silanol group affected the condensation process of Si-OH group during the formation of mesoporous structure, resulting in the increase of Si-OH group number on the surface of MCM-48. Compared with MCM-48 synthesized by single template (CTAB), the acetone adsorption capacity of MCM-48 (1:3) synthesized by mixed templates (PVP:CTAB = 1:3) improved by 23.86%, which was attributed to the increase of silanol group amount and the decrease of pore size. In addition, Bangham model had the highest goodness of fit to describe the adsorption process among four kinetic models for the adsorbents, conforming to the mechanism of pore diffusion. The Langmuir and Freundlich models were used to fit the adsorption isotherm data, and the Freundlich model could better describe the adsorption of acetone. Freundlich model fitting results showed that MCM-48 with rich silanol had a strong affinity for acetone, and the adsorption of acetone on MCM-48 belonged to multilayer adsorption. The thermodynamic results showed that the adsorption of MCM-48 for acetone was physical adsorption, and the adsorption behavior was exothermic. This work provided insight into how the inherent properties of an adsorbent and environmental factors (including initial concentration and adsorption temperature) affected the adsorption performance of ketones, thus more ideas could be provided for the accurate design of adsorbents. Furthermore, silanol-rich MCM-48 synthesized by mixed templates is expected to be a promising adsorbent for acetone removal.", "gemini": "Silica mesoporous MCM-48 với silanol phong phú đã được điều chế bằng cách sử dụng polyvinylpyrrolidone (PVP) và cetyltrimethylammonium bromide (CTAB) làm khuôn mẫu hỗn hợp và hiệu suất hấp phụ động của acetone được đánh giá bằng cách thử nghiệm đường cong đột phá. Micelle hỗn hợp được hình thành bởi CTAB và PVP, cũng như liên kết hydro giữa nhóm cacbonyl của PVP và nhóm silanol ảnh hưởng đến quá trình ngưng tụ của nhóm Si-OH trong quá trình hình thành cấu trúc mesoporous, dẫn đến sự gia tăng số lượng nhóm Si-OH trên bề mặt của MCM-48. So với MCM-48 được tổng hợp bằng khuôn mẫu đơn (CTAB), khả năng hấp phụ acetone của MCM-48 (1:3) được tổng hợp bằng khuôn mẫu hỗn hợp (PVP:CTAB = 1:3) được cải thiện 23,86%, nguyên nhân là do lượng nhóm silanol tăng và kích thước lỗ giảm. Ngoài ra, mô hình Bangham có độ phù hợp tốt nhất để mô tả quá trình hấp phụ trong số bốn mô hình động học cho chất hấp phụ, phù hợp với cơ chế khuếch tán qua lỗ. Các mô hình Langmuir và Freundlich được sử dụng để phù hợp với dữ liệu đẳng nhiệt hấp phụ và mô hình Freundlich có thể mô tả tốt hơn sự hấp phụ của acetone. Kết quả phù hợp với mô hình Freundlich cho thấy MCM-48 với silanol phong phú có ái lực mạnh với acetone và sự hấp phụ của acetone trên MCM-48 thuộc loại hấp phụ đa lớp. Kết quả nhiệt động lực học cho thấy sự hấp phụ của MCM-48 đối với acetone là hấp phụ vật lý và hành vi hấp phụ là tỏa nhiệt. Công trình này cung cấp thông tin chi tiết về cách các đặc tính vốn có của chất hấp phụ và các yếu tố môi trường (bao gồm nồng độ ban đầu và nhiệt độ hấp phụ) ảnh hưởng đến hiệu suất hấp phụ của ketone, do đó có thể cung cấp nhiều ý tưởng hơn cho việc thiết kế chính xác các chất hấp phụ. Hơn nữa, MCM-48 giàu silanol được tổng hợp bằng khuôn mẫu hỗn hợp dự kiến sẽ là chất hấp phụ đầy hứa hẹn để loại bỏ acetone."} +{"idx": 1880012, "en": "A palladium/XantPhos-catalyzed [5 + 2] annulation of VECs with electron-deficient alkenes having an isolated carbon-carbon double bond has been developed to afford spirobarbiturate-tetrahydrooxepines. This study provides an expedient assembly of biologically interesting spirobarbiturate-tetrahydrooxepines. The easy scalability and versatile transformability of the reaction products were also exhibited.", "gemini": "Một vòng annulation [5 + 2] xúc tác bởi palladium/XantPhos của VEC với anken thiếu điện tử có liên kết đôi carbon-carbon cô lập đã được phát triển để tạo ra spirobarbiturat-tetrahydrooxepin. Nghiên cứu này cung cấp một phương pháp lắp ráp nhanh chóng các spirobarbiturat-tetrahydrooxepin thú vị về mặt sinh học. Tính dễ mở rộng và khả năng biến đổi linh hoạt của các sản phẩm phản ứng cũng đã được thể hiện."} +{"idx": 1880013, "en": "INTRODUCTION Subcutaneous sumatriptan, a 5HT1B/1D agonist, is the most effective drug in cluster headache acute treatment. About 25% of the patients do not respond to subcutaneous sumatriptan; the reasons for this are unknown. In this study, we compare clinical characteristics of cluster headache patients responding and non-responding to subcutaneous sumatriptan. METHODS We retrospectively investigated the clinical records of 277 cluster headache patients. Patients reporting repeated satisfactory response to subcutaneous sumatriptan within 15 minutes were considered responders. RESULTS Of 206 cluster headache patients who had used subcutaneous sumatriptan (mean age 45.6, 16% females, 48% chronic), 91% were responders, and 9% non-responders. Compared to responders, non-responders had longer and more frequent attacks: 60 (median; IQR 38-90) vs. 100 (60-120) minutes (p = 0.028), 4 (2.5-5) vs. 3 (2-4) attacks/day (p = 0.024). No other difference was found. CONCLUSIONS In cluster headache attacks with long duration and high frequency, pain mechanisms not involving 5HT1B/1D receptors may play a more relevant role.", "gemini": "GIỚI THIỆU Sumatriptan dưới da, một chất chủ vận 5HT1B/1D, là loại thuốc hiệu quả nhất trong điều trị cấp tính đau đầu từng cơn. Khoảng 25% bệnh nhân không đáp ứng với sumatriptan dưới da; lý do cho điều này vẫn chưa được biết. Trong nghiên cứu này, chúng tôi so sánh các đặc điểm lâm sàng của bệnh nhân đau đầu từng cơn đáp ứng và không đáp ứng với sumatriptan dưới da. PHƯƠNG PHÁP Chúng tôi đã điều tra hồi cứu hồ sơ lâm sàng của 277 bệnh nhân đau đầu từng cơn. Những bệnh nhân báo cáo đáp ứng tốt lặp lại với sumatriptan dưới da trong vòng 15 phút được coi là những người đáp ứng. KẾT QUẢ Trong số 206 bệnh nhân đau đầu từng cơn đã sử dụng sumatriptan dưới da (tuổi trung bình 45,6, 16% nữ, 48% mạn tính), 91% là người đáp ứng và 9% không đáp ứng. So với những người đáp ứng, những người không đáp ứng có các cơn đau kéo dài và thường xuyên hơn: 60 (trung vị; IQR 38-90) so với 100 (60-120) phút (p = 0,028), 4 (2,5-5) so với 3 (2-4) cơn/ngày (p = 0,024). Không tìm thấy sự khác biệt nào khác. KẾT LUẬN Trong các cơn đau đầu từng cơn kéo dài và tần suất cao, các cơ chế đau không liên quan đến thụ thể 5HT1B/1D có thể đóng vai trò quan trọng hơn."} +{"idx": 1880014, "en": "OBJECTIVE Tensions about the definition, diagnostics, and role of psychological trauma in psychiatry are long-standing. This study sought to explore what metaphor patterns in qualitative interviews may reveal about the beliefs of psychiatrists in relation to trauma. METHOD A qualitative inquiry using systematic metaphor analysis of 13 in-depth interviews with Australian psychiatrists. RESULTS Three themes were identified: a power struggle between people, trauma, and psychiatry; trauma is not a medical condition; and serving the profession to protect society. CONCLUSIONS Metaphors present trauma as a powerful force that people can manage in different ways. Psychiatrists may view trauma as a social rather than medical issue. Psychiatrists experience role pressure associated with trauma including incongruence with risk management expectations of their roles.", "gemini": "MỤC TIÊU Những căng thẳng về định nghĩa, chẩn đoán và vai trò của chấn thương tâm lý trong tâm thần học đã tồn tại từ lâu. Nghiên cứu này tìm cách khám phá những kiểu ẩn dụ nào trong các cuộc phỏng vấn định tính có thể tiết lộ về niềm tin của các bác sĩ tâm thần liên quan đến chấn thương. PHƯƠNG PHÁP Một cuộc điều tra định tính sử dụng phân tích ẩn dụ có hệ thống của 13 cuộc phỏng vấn chuyên sâu với các bác sĩ tâm thần người Úc. KẾT QUẢ Ba chủ đề đã được xác định: cuộc đấu tranh quyền lực giữa con người, chấn thương và tâm thần học; chấn thương không phải là một tình trạng y tế; và phục vụ nghề nghiệp để bảo vệ xã hội. KẾT LUẬN Các ẩn dụ thể hiện chấn thương như một lực mạnh mẽ mà mọi người có thể quản lý theo nhiều cách khác nhau. Các bác sĩ tâm thần có thể coi chấn thương là một vấn đề xã hội hơn là vấn đề y tế. Các bác sĩ tâm thần trải qua áp lực về vai trò liên quan đến chấn thương bao gồm sự không phù hợp với kỳ vọng quản lý rủi ro của vai trò của họ."} +{"idx": 1880015, "en": "The use of contrast agents as signal enhancers during ultrasound improves visualization and the diagnostic utility of this technology in medical imaging. Although widely used in many disciplines, contrast ultrasound is not routinely implemented in obstetrics, largely due to safety concerns of administered agents for pregnant women and the limited number of studies that address this issue. Here the microbubble characteristics that make them beneficial for enhancement of the blood pool and the quantification of real-time imaging are reviewed. Literature from pregnant animal model studies and safety assessments are detailed, and the potential for contrast-enhanced ultrasound to provide clinically relevant data and benefit our understanding of early placental development and detection of placental dysfunction is discussed.", "gemini": "Việc sử dụng các tác nhân tạo độ tương phản như chất tăng cường tín hiệu trong quá trình siêu âm giúp cải thiện khả năng hiển thị và tiện ích chẩn đoán của công nghệ này trong hình ảnh y tế. Mặc dù được sử dụng rộng rãi trong nhiều lĩnh vực, nhưng siêu âm tạo độ tương phản không được thực hiện thường quy trong sản khoa, chủ yếu là do lo ngại về tính an toàn của các tác nhân được dùng cho phụ nữ mang thai và số lượng nghiên cứu giải quyết vấn đề này còn hạn chế. Tại đây, các đặc điểm của bong bóng siêu nhỏ giúp chúng có lợi cho việc tăng cường lưu lượng máu và định lượng hình ảnh thời gian thực được xem xét. Các tài liệu từ các nghiên cứu trên mô hình động vật mang thai và đánh giá an toàn được trình bày chi tiết và tiềm năng của siêu âm tăng cường độ tương phản để cung cấp dữ liệu có liên quan về mặt lâm sàng và có lợi cho việc hiểu biết của chúng ta về sự phát triển nhau thai sớm và phát hiện rối loạn chức năng nhau thai được thảo luận."} +{"idx": 1880016, "en": "Phosphate limitation has been suggested as a preventive method against biofilms. P-limited feed water was studied as a preventive strategy against biofouling in cooling towers (CTs). Three pilot-scale open recirculating CTs were operated in parallel for five weeks. RO permeate was fed to the CTs (1) without supplementation (reference), (2) with supplementation by biodegradable carbon (P-limited) and (3) with supplementation of all nutrients (non-P-limited). The P-limited water contained ≤10 µg PO4 l-1. Investigating the CT-basins and coupons showed that P-limited water (1) did not prevent biofilm formation and (2) resulted in a higher volume of organic matter per unit of active biomass compared with the other CTs. Exposure to external conditions and cycle of concentration were likely factors that allowed a P concentration sufficient to cause extensive biofouling despite being the limiting compound. In conclusion, phosphate limitation in cooling water is not a suitable strategy for CT biofouling control.", "gemini": "Hạn chế phosphate đã được đề xuất như một phương pháp phòng ngừa chống lại màng sinh học. Nước cấp hạn chế P đã được nghiên cứu như một chiến lược phòng ngừa chống lại sự bám bẩn sinh học trong các tháp giải nhiệt (CT). Ba CT tuần hoàn hở quy mô thí điểm đã được vận hành song song trong năm tuần. Nước thẩm thấu ngược được cấp cho các CT (1) không bổ sung (tham chiếu), (2) bổ sung bằng cacbon phân hủy sinh học (hạn chế P) và (3) bổ sung tất cả các chất dinh dưỡng (không hạn chế P). Nước hạn chế P chứa ≤10 µg PO4 l-1. Việc điều tra các bể CT và phiếu giảm giá cho thấy nước hạn chế P (1) không ngăn ngừa sự hình thành màng sinh học và (2) dẫn đến lượng chất hữu cơ trên một đơn vị sinh khối hoạt động cao hơn so với các CT khác. Tiếp xúc với các điều kiện bên ngoài và chu trình cô đặc có thể là những yếu tố cho phép nồng độ P đủ để gây ra sự bám bẩn sinh học rộng rãi mặc dù là hợp chất giới hạn. Tóm lại, hạn chế phosphate trong nước làm mát không phải là một chiến lược phù hợp để kiểm soát sự bám bẩn sinh học của CT."} +{"idx": 1880017, "en": "Venous thromboembolism, occlusion of dialysis catheters, circuit thrombosis in ECMO devices, all in the face of prophylactic and sometimes even therapeutic anti-coagulation, are frequent features of COVID-19 coagulopathy. The trials available to guide clinicians are methodologically limited. There are several unresolved controversies including 1) Should all hospitalized patients with COVID-19 receive prophylactic anti-coagulation? 2) Which patients should have their dosage escalated to intermediate dose? 3) Which patients should be considered for full-dose anti-coagulation even without a measurable thromboembolic event and how should that anti-coagulation be monitored? 4) Should patients receive post-discharge anti-coagulation? 5) What thrombotic issues are related to the various medications being used to treat this coagulopathy? 6) Is anti-phospholipid anti-body part of this syndrome? 7) How do the different treatments for this disease impact the coagulation issues? The aims of this article are to explore these questions and interpret the available data based on the current evidence.", "gemini": "Thuyên tắc huyết khối tĩnh mạch, tắc nghẽn ống thông lọc máu, huyết khối mạch trong các thiết bị ECMO, tất cả đều xảy ra khi đã có các biện pháp chống đông dự phòng và đôi khi thậm chí là điều trị, là những đặc điểm thường gặp của bệnh lý đông máu COVID-19. Các thử nghiệm có sẵn để hướng dẫn các bác sĩ lâm sàng có phương pháp hạn chế. Có một số tranh cãi chưa được giải quyết bao gồm 1) Tất cả bệnh nhân nhập viện vì COVID-19 có nên được chống đông dự phòng không? 2) Nên tăng liều cho những bệnh nhân nào lên liều trung gian? 3) Những bệnh nhân nào nên được xem xét để chống đông toàn phần ngay cả khi không có sự kiện huyết khối tắc mạch có thể đo lường được và nên theo dõi quá trình chống đông đó như thế nào? 4) Bệnh nhân có nên được chống đông sau khi xuất viện không? 5) Các vấn đề về huyết khối liên quan đến các loại thuốc khác nhau đang được sử dụng để điều trị bệnh lý đông máu này là gì? 6) Có phải kháng thể chống phospholipid là một phần của hội chứng này không? 7) Các phương pháp điều trị khác nhau cho căn bệnh này ảnh hưởng đến các vấn đề về đông máu như thế nào? Mục đích của bài viết này là khám phá những câu hỏi này và diễn giải dữ liệu có sẵn dựa trên các bằng chứng hiện tại."} +{"idx": 1880018, "en": "OBJECTIVE To study the effects of disability, cognitive impairment, and neuropsychiatric disturbance among older Mexican Americans on depressive symptoms in their children caregivers. METHODS This study utilizes data from Wave 7 (2010-2011) of the Hispanic Established Populations for the Epidemiologic Study of the Elderly (HEPESE). The final sample included 200 adult children caregivers that provided direct personal care with activities of daily living (ADL) (e.g. bathing, toileting, dressing, etc.) to their older parents (average age = 87). We analyzed the influence of ADL disability, cognition (MMSE), and neuropsychiatric symptoms (NPI) of the care recipient on depressive symptoms of the adult child caregiver. A cross-sectional multivariable linear regression analysis was conducted to examine the effect of neuropsychiatric disturbance on caregiver depressive symptoms. RESULTS Presence of care recipient NPI symptoms was associated with higher depressive symptoms for caregivers. Additional characteristics associated with caregiver depressive symptoms were not being married, and higher perceived social stress. ADL disability of the care recipient, cognitive functioning of the care recipient, or caregiver health status alone did not have a significant effect on depressive symptoms of the caregiver. CONCLUSIONS In a Mexican American familistic culture, disability and cognitive impairment might be better tolerated by families but neuropsychiatric behavioral symptoms related to dementia may take an increased toll on family member caregivers. The need to provide respite services, mental health resources and community services for caregivers of care recipients with neuropsychiatric symptoms is of paramount importance to alleviate depressive symptoms and burden among caregivers.", "gemini": "MỤC TIÊU Nghiên cứu các ảnh hưởng của tình trạng khuyết tật, suy giảm nhận thức và rối loạn tâm thần kinh ở người Mỹ gốc Mexico lớn tuổi đối với các triệu chứng trầm cảm ở những người chăm sóc con cái của họ. PHƯƠNG PHÁP Nghiên cứu này sử dụng dữ liệu từ Đợt 7 (2010-2011) của Dự án Nghiên cứu Dịch tễ học về Người cao tuổi (HEPESE) của Cộng đồng Người gốc Tây Ban Nha. Mẫu cuối cùng bao gồm 200 người chăm sóc là con trưởng thành, những người đã cung cấp dịch vụ chăm sóc cá nhân trực tiếp với các hoạt động sinh hoạt hàng ngày (ADL) (ví dụ: tắm rửa, vệ sinh, mặc quần áo, v.v.) cho cha mẹ già của họ (tuổi trung bình = 87). Chúng tôi đã phân tích ảnh hưởng của tình trạng khuyết tật ADL, nhận thức (MMSE) và các triệu chứng tâm thần kinh (NPI) của người được chăm sóc đối với các triệu chứng trầm cảm của người chăm sóc là con trưởng thành. Một phân tích hồi quy tuyến tính đa biến theo chiều cắt ngang đã được tiến hành để kiểm tra tác động của rối loạn tâm thần kinh đối với các triệu chứng trầm cảm của người chăm sóc. KẾT QUẢ Sự hiện diện của các triệu chứng NPI của người được chăm sóc có liên quan đến các triệu chứng trầm cảm cao hơn ở người chăm sóc. Các đặc điểm bổ sung liên quan đến các triệu chứng trầm cảm của người chăm sóc là không kết hôn và căng thẳng xã hội cao hơn. Tình trạng khuyết tật ADL của người được chăm sóc, chức năng nhận thức của người được chăm sóc hoặc tình trạng sức khỏe của người chăm sóc không ảnh hưởng đáng kể đến các triệu chứng trầm cảm của người chăm sóc. KẾT LUẬN Trong một nền văn hóa gia đình của người Mỹ gốc Mexico, tình trạng khuyết tật và suy giảm nhận thức có thể được gia đình dung nạp tốt hơn nhưng các triệu chứng hành vi tâm thần kinh liên quan đến chứng mất trí có thể gây ra gánh nặng gia tăng cho những người chăm sóc trong gia đình. Nhu cầu cung cấp các dịch vụ chăm sóc tạm thời, các nguồn lực về sức khỏe tâm thần và các dịch vụ cộng đồng cho những người chăm sóc những người được chăm sóc có các triệu chứng tâm thần kinh là vô cùng quan trọng để giảm bớt các triệu chứng trầm cảm và gánh nặng cho những người chăm sóc."} +{"idx": 1880019, "en": "Pulled-in-two syndrome (PITS) is a serious intraoperative complication of strabismus surgery in which an extraocular muscle manipulated during the procedure is ruptured and potentially lost. Usually, there is a systemic or local condition that determines muscle weakness when put under tension. If the proximal portion of the broken muscle can be found, it can be reattached to the ocular globe or remaining muscle. If this is not possible, there are multiple varying approaches. We present three cases of PITS of the inferior rectus muscle, treated with good results with anterior and nasal transposition of the inferior oblique muscle. We propose this surgery as another potential technique if the muscle can not be retrieved.", "gemini": "Hội chứng kéo căng (PITS) là một biến chứng trong phẫu thuật nội khoa nghiêm trọng của phẫu thuật lác, trong đó một cơ mắt ngoài bị thao tác trong quá trình phẫu thuật bị đứt và có khả năng bị mất. Thông thường, có một tình trạng toàn thân hoặc tại chỗ xác định tình trạng yếu cơ khi bị căng. Nếu có thể tìm thấy phần gần của cơ bị đứt, thì có thể gắn lại vào nhãn cầu hoặc cơ còn lại. Nếu không thể thực hiện, có nhiều phương pháp tiếp cận khác nhau. Chúng tôi trình bày ba trường hợp PITS của cơ thẳng dưới, được điều trị với kết quả tốt với sự chuyển vị trước và mũi của cơ chéo dưới. Chúng tôi đề xuất phương pháp phẫu thuật này như một kỹ thuật tiềm năng khác nếu không thể lấy lại cơ."} +{"idx": 1880020, "en": "A 47-year-old man was admitted to the clinic with histologically diagnosed thymoma of the anterior mediastinum, pT3N0M1a, stage IYA, type B3. He underwent surgery for primary tumor resection through a median sternotomy and left thoracotomy at the 7th intercostal space to remove pleural metastases. On the first postoperative day, massive bleeding occurred, a resternotomy was carried out but failed to save the patient. A fracture of the right first rib, which injured the right vertebral artery, had caused massive bleeding and was diagnosed at autopsy. Surgeons should keep in mind this potentially fatal complication of a median sternotomy.", "gemini": "Một người đàn ông 47 tuổi được nhập viện với chẩn đoán mô học là u tuyến ức ở trung thất trước, pT3N0M1a, giai đoạn IYA, loại B3. Ông đã trải qua phẫu thuật cắt bỏ khối u nguyên phát qua đường mổ xương ức giữa và mở ngực trái ở khoang liên sườn thứ 7 để cắt bỏ các di căn màng phổi. Vào ngày đầu tiên sau phẫu thuật, đã xảy ra tình trạng chảy máu ồ ạt, một cuộc mổ lại xương ức đã được thực hiện nhưng không cứu được bệnh nhân. Một vết nứt ở xương sườn đầu tiên bên phải, làm tổn thương động mạch đốt sống phải, đã gây ra tình trạng chảy máu ồ ạt và được chẩn đoán khi khám nghiệm tử thi. Các bác sĩ phẫu thuật nên ghi nhớ biến chứng có khả năng gây tử vong này của một cuộc mổ xương ức giữa."} +{"idx": 1880021, "en": "BACKGROUND/OBJECTIVE Perinatal mental health services are a current NHS priority and services are being increased for women. There is limited research on mothers' perspectives of these services and most research focuses on mother and baby units (MBUs). This study explored women's views of their experiences of generic wards, MBUs and crisis resolution teams. METHODS A qualitative thematic analysis was conducted on written feedback on a service-user-designed questionnaire. One hundred and thirty-nine women recruited across 42 mental health trusts made comments. RESULTS Two key themes were identified: support networks and staff authority. Support networks included subthemes relating to families, peers and staff. The theme of staff authority incorporated subthemes about communication, confidence in staff and service-user autonomy. All themes contributed to whether mothers felt safe in these services. Mothers reported the benefits of positive, non-coercive relationships with family and staff for their recovery. The findings highlight that the challenges women face in perinatal settings reflect the literature on general psychiatric services, particularly around coercion. CONCLUSIONS Specific implications for mothers accessing perinatal mental health services: 1) integrated mental health care and support with babies; 2) support with separation from babies for mothers in acute wards; 3) improvement of women's relationships with social services across all services.", "gemini": "BỐI CẢNH/MỤC TIÊU Các dịch vụ sức khỏe tâm thần tiền sản là ưu tiên hiện tại của NHS và các dịch vụ đang được tăng cường cho phụ nữ. Có rất ít nghiên cứu về quan điểm của các bà mẹ về những dịch vụ này và hầu hết các nghiên cứu tập trung vào các đơn vị mẹ và bé (MBU). Nghiên cứu này khám phá quan điểm của phụ nữ về kinh nghiệm của họ ở các khoa tổng quát, MBU và nhóm giải quyết khủng hoảng. PHƯƠNG PHÁP Một phân tích chủ đề định tính đã được thực hiện trên phản hồi bằng văn bản trên một bảng câu hỏi do người sử dụng dịch vụ thiết kế. Một trăm ba mươi chín phụ nữ được tuyển dụng trên 42 quỹ tín thác sức khỏe tâm thần đã đưa ra bình luận. KẾT QUẢ Hai chủ đề chính đã được xác định: mạng lưới hỗ trợ và thẩm quyền của nhân viên. Mạng lưới hỗ trợ bao gồm các chủ đề phụ liên quan đến gia đình, bạn bè và nhân viên. Chủ đề thẩm quyền của nhân viên kết hợp các chủ đề phụ về giao tiếp, sự tự tin vào nhân viên và quyền tự chủ của người sử dụng dịch vụ. Tất cả các chủ đề đều góp phần vào việc liệu các bà mẹ có cảm thấy an toàn trong những dịch vụ này hay không. Các bà mẹ báo cáo những lợi ích của các mối quan hệ tích cực, không cưỡng chế với gia đình và nhân viên để phục hồi sức khỏe. Những phát hiện này nhấn mạnh rằng những thách thức mà phụ nữ phải đối mặt trong các cơ sở tiền sản phản ánh tài liệu về các dịch vụ tâm thần nói chung, đặc biệt là xung quanh sự cưỡng chế. KẾT LUẬN Các hàm ý cụ thể đối với các bà mẹ tiếp cận các dịch vụ sức khỏe tâm thần tiền sản: 1) chăm sóc sức khỏe tâm thần tích hợp và hỗ trợ với trẻ sơ sinh; 2) hỗ trợ tách trẻ sơ sinh khỏi bà mẹ ở các khoa cấp tính; 3) cải thiện mối quan hệ của phụ nữ với các dịch vụ xã hội trên tất cả các dịch vụ."} +{"idx": 1880022, "en": "A number of clinical and radiological predictors of either stone impaction or ureteral stone passage (SP) have been proposed. We aimed at identifying the key predictors of successful SP by using readily available CT-based tools/measurements. Patients presenting to the emergency department from February 2017 to February 2018 with an acute unilateral ureteral stone confirmed on non-contrast CT and managed conservatively were followed for SP. Patients with renal impairment, sepsis or requiring emergent intervention were excluded. Patients were followed at 1 month to confirm SP (stone collection/repeat imaging) or failure of passage. The CT variables analyzed included: Stone factors [location, size, volume, HU density (HUD)], impaction factors [ureteral HUD above and below the stone, maximal ureteral wall thickness (UWT) at the stone site, contralateral UWT, and ureteral diameter above and below the stone]. Binary logistic regression analysis was performed to identify predictors of SP. Forty-nine patients met study inclusion criteria, of whom 32 (65.3%) passed the stone without further intervention. Patients with successful passage were more likely to have smaller, lower volume and less dense stones located in the distal ureter (p < 0.01). Lower ureteral HUD below the stone, lower maximal UWT, and lower ureteral diameter above the stone were associated with successful passage (p < 0.01). On multivariable logistic regression analysis, only maximal UWT at the stone site was a significant independent predictor of SP outcome (p = 0.01). Youden's criterion identified 2.3 mm as the optimal UWT cut-off point, which will accurately predict SP with 82.4% sensitivity and 87.5% specificity. Maximal UWT at the stone site was the most significant predictor of successful passage in acute unilateral ureteral stones, with an optimal cut-off point of 2.3 mm. Further prospective studies are needed to accurately predict spontaneous SP.", "gemini": "Một số yếu tố dự báo lâm sàng và X quang về tắc sỏi hoặc thông sỏi niệu quản (SP) đã được đề xuất. Chúng tôi nhằm mục đích xác định các yếu tố dự báo chính của SP thành công bằng cách sử dụng các công cụ/phép đo dựa trên CT có sẵn. Những bệnh nhân đến khoa cấp cứu từ tháng 2 năm 2017 đến tháng 2 năm 2018 bị sỏi niệu quản đơn bên cấp tính được xác nhận trên CT không cản quang và được điều trị bảo tồn đã được theo dõi để phát hiện SP. Những bệnh nhân bị suy thận, nhiễm trùng huyết hoặc cần can thiệp cấp cứu đã bị loại trừ. Bệnh nhân được theo dõi sau 1 tháng để xác nhận SP (lấy sỏi/chụp lại hình ảnh) hoặc không thông. Các biến CT được phân tích bao gồm: Các yếu tố về sỏi [vị trí, kích thước, thể tích, mật độ HU (HUD)], các yếu tố tắc nghẽn [HUD niệu quản phía trên và dưới sỏi, độ dày thành niệu quản tối đa (UWT) tại vị trí sỏi, UWT đối bên và đường kính niệu quản phía trên và dưới sỏi]. Phân tích hồi quy logistic nhị phân đã được thực hiện để xác định các yếu tố dự báo SP. Bốn mươi chín bệnh nhân đáp ứng các tiêu chí đưa vào nghiên cứu, trong số đó có 32 (65,3%) đã thông sỏi mà không cần can thiệp thêm. Những bệnh nhân thông sỏi thành công có nhiều khả năng có sỏi nhỏ hơn, thể tích nhỏ hơn và ít đặc hơn nằm ở niệu quản xa (p < 0,01). HUD niệu quản thấp hơn dưới sỏi, UWT tối đa thấp hơn và đường kính niệu quản thấp hơn phía trên sỏi có liên quan đến việc thông sỏi thành công (p < 0,01). Trên phân tích hồi quy logistic đa biến, chỉ có UWT tối đa tại vị trí sỏi là yếu tố dự báo độc lập có ý nghĩa đối với kết quả SP (p = 0,01). Tiêu chuẩn của Youden xác định 2,3 mm là điểm cắt UWT tối ưu, có thể dự đoán chính xác SP với độ nhạy 82,4% và độ đặc hiệu 87,5%. UWT tối đa tại vị trí sỏi là yếu tố dự báo quan trọng nhất về việc thông sỏi thành công ở sỏi niệu quản đơn bên cấp tính, với điểm cắt tối ưu là 2,3 mm. Cần có thêm các nghiên cứu triển vọng để dự đoán chính xác SP tự phát."} +{"idx": 1880023, "en": "BACKGROUND For patients presenting with ST-segment-elevation myocardial infarction, national quality initiatives monitor hospitals' proportion of cases with door-to-balloon (D2B) time under 90 minutes. Hospitals are allowed to exclude patients from reporting and may modify behavior to improve performance. We sought to identify whether there is a discontinuity in the number of cases included in the D2B time metric at 90 minutes and whether operators were increasingly likely to pursue femoral access in patients with less time to meet the 90-minute quality metric. METHODS Adult patients with ST-segment-elevation myocardial infarction undergoing primary percutaneous coronary intervention from 2011 to 2018 were identified from the Cardiac Care Outcomes Assessment Program, a quality improvement registry in Washington state. We used the regression discontinuity framework to test for discontinuity at 90 minutes among the included cases. We defined a novel variable, remaining D2B as 90 minutes minus the time between hospital arrival and catheterization laboratory arrival. We estimated multivariable logistic regression models to assess the relationship between remaining D2B time and access site. RESULTS A total of 19 348 patients underwent primary percutaneous coronary intervention and were included in the analysis. Overall, 7436 (38.4%) were excluded from the metric. There appeared to be a visual discontinuity in included cases around 90 minutes; however, local quadratic regression around the 90-minute cutoff did not reveal evidence of a significant discontinuity (P=0.66). Multivariable analysis showed no significant relationship between remaining D2B time and the odds of undergoing femoral access (P=0.73). CONCLUSIONS Among patients undergoing percutaneous coronary intervention for ST-segment-elevation myocardial infarction, we did not find evidence of a statistically significant discontinuity in the frequency of included cases around 90 minutes or an increased preference for femoral access correlated with decreasing time to meet the 90-minute D2B time quality metric. Together, these findings indicate no evidence of widespread inappropriate methods to improve performance on D2B time metrics.", "gemini": "BỐI CẢNH Đối với những bệnh nhân có nhồi máu cơ tim ST chênh lệch đoạn, các sáng kiến chất lượng quốc gia theo dõi tỷ lệ các trường hợp có thời gian từ cửa đến bóng (D2B) dưới 90 phút của các bệnh viện. Các bệnh viện được phép loại trừ bệnh nhân khỏi báo cáo và có thể thay đổi hành vi để cải thiện hiệu suất. Chúng tôi tìm cách xác định xem có sự gián đoạn nào về số lượng các trường hợp được đưa vào số liệu thời gian D2B ở phút thứ 90 hay không và liệu các bác sĩ phẫu thuật có ngày càng có khả năng tiếp cận động mạch đùi ở những bệnh nhân có ít thời gian hơn để đáp ứng số liệu chất lượng 90 phút hay không. PHƯƠNG PHÁP Những bệnh nhân trưởng thành bị nhồi máu cơ tim ST chênh lệch đoạn trải qua can thiệp động mạch vành qua da chính từ năm 2011 đến năm 2018 đã được xác định từ Chương trình Đánh giá Kết quả Chăm sóc Tim mạch, một sổ đăng ký cải tiến chất lượng tại tiểu bang Washington. Chúng tôi đã sử dụng khung hồi quy gián đoạn để kiểm tra sự gián đoạn ở phút thứ 90 trong số các trường hợp được đưa vào. Chúng tôi đã định nghĩa một biến mới, D2B còn lại là 90 phút trừ đi thời gian giữa lúc đến bệnh viện và đến phòng thông tim. Chúng tôi ước tính các mô hình hồi quy logistic đa biến để đánh giá mối quan hệ giữa thời gian D2B còn lại và vị trí tiếp cận. KẾT QUẢ Tổng cộng có 19.348 bệnh nhân đã trải qua can thiệp động mạch vành qua da chính và được đưa vào phân tích. Nhìn chung, 7436 (38,4%) đã bị loại khỏi số liệu. Có vẻ như có sự gián đoạn trực quan trong các trường hợp được đưa vào xung quanh phút thứ 90; tuy nhiên, hồi quy bậc hai cục bộ xung quanh ngưỡng 90 phút không cho thấy bằng chứng về sự gián đoạn đáng kể (P = 0,66). Phân tích đa biến cho thấy không có mối quan hệ đáng kể giữa thời gian D2B còn lại và tỷ lệ thực hiện tiếp cận động mạch đùi (P = 0,73). KẾT LUẬN Trong số những bệnh nhân trải qua can thiệp động mạch vành qua da đối với nhồi máu cơ tim ST chênh lệch đoạn, chúng tôi không tìm thấy bằng chứng về sự gián đoạn có ý nghĩa thống kê về tần suất các trường hợp được đưa vào xung quanh phút thứ 90 hoặc sở thích ngày càng tăng đối với việc tiếp cận động mạch đùi có liên quan đến việc giảm thời gian để đáp ứng số liệu chất lượng thời gian D2B 90 phút. Cùng nhau, những phát hiện này chỉ ra không có bằng chứng về các phương pháp không phù hợp phổ biến để cải thiện hiệu suất trên các số liệu thời gian D2B."} +{"idx": 1880024, "en": "BACKGROUND Estimated glucose disposal rate (eGDR) is a practical measure of Insulin Resistance (IR) which can be easily incorporated into clinical practice. We profiled eGDR in younger adults with type 1 diabetes mellitus (T1DM) by their demographic and clinical characteristics. METHODS In this single centre study, medical records of TIDM were assessed and eGDR tertiles correlated with demographic and clinical variables. RESULTS Of 175 T1DM individuals, 108 (61.7%) were males. Mean age (±SD) was 22.0 ± 1.6 years and median time from diagnosis 11.0 years (range 1-23). Individuals were predominantly Caucasian (81.7%), with 27.4% being overweight (BMI: 25-30 kg/m2) and 13.7% obese (BMI > 30 kg/m2). Mean total cholesterol (TC) levels were significantly lower in high and middle eGDR tertiles (4.4 ± 1 and 4.3 ± 0.8 mmol/l, respectively) compared with low eGDR tertile (4.8 ± 1, p < 0.05 for both). Triglyceride (TG) levels showed a similar trend at 1.1 ± 0.5 and 1.1 ± 0.5 mmol/l for high and middle eGDR tertile compared to low eGDR tertile (1.5 ± 1 mmol/l, p < 0.05 for both). Renal function was similar across eGDR tertiles and no difference in retinopathy was detected. CONCLUSION TC and TG are altered in individuals with T1DM and low eGDR, suggesting that this subgroup requires optimal lipid management to ameliorate their vascular risk.", "gemini": "BỐI CẢNH Tỷ lệ thải glucose ước tính (eGDR) là một biện pháp thực tế để Đánh giá tình trạng kháng Insulin (IR) có thể dễ dàng đưa vào thực hành lâm sàng. Chúng tôi đã lập hồ sơ eGDR ở những người trưởng thành trẻ mắc bệnh đái tháo đường týp 1 (T1DM) theo đặc điểm nhân khẩu học và lâm sàng của họ. PHƯƠNG PHÁP Trong nghiên cứu đơn trung tâm này, hồ sơ bệnh án của TIDM đã được đánh giá và các phần ba eGDR có tương quan với các biến số nhân khẩu học và lâm sàng. KẾT QUẢ Trong số 175 cá nhân mắc T1DM, có 108 (61,7%) là nam giới. Tuổi trung bình (±SD) là 22,0 ± 1,6 tuổi và thời gian trung bình từ khi chẩn đoán là 11,0 năm (phạm vi 1-23). Những cá nhân này chủ yếu là người da trắng (81,7%), trong đó 27,4% thừa cân (BMI: 25-30 kg/m2) và 13,7% béo phì (BMI> 30 kg/m2). Nồng độ cholesterol toàn phần (TC) trung bình thấp hơn đáng kể ở phần ba eGDR cao và trung bình (lần lượt là 4,4 ± 1 và 4,3 ± 0,8 mmol/l) so với phần ba eGDR thấp (4,8 ± 1, p < 0,05 cho cả hai). Nồng độ Triglyceride (TG) cho thấy xu hướng tương tự ở mức 1,1 ± 0,5 và 1,1 ± 0,5 mmol/l đối với phần ba eGDR cao và trung bình so với phần ba eGDR thấp (1,5 ± 1 mmol/l, p < 0,05 cho cả hai). Chức năng thận tương tự nhau ở các phần ba eGDR và không phát hiện thấy sự khác biệt về bệnh võng mạc. KẾT LUẬN TC và TG bị thay đổi ở những cá nhân mắc T1DM và eGDR thấp, điều này cho thấy nhóm phụ này cần quản lý lipid tối ưu để cải thiện nguy cơ mạch máu của họ."} +{"idx": 1880025, "en": "The ongoing coronavirus disease 2019 pandemic has resulted in additional challenges for systems designed to perform expeditious primary percutaneous coronary intervention for patients presenting with ST-segment-elevation myocardial infarction. There are 2 important considerations: the guideline-recommended time goals were difficult to achieve for many patients in high-income countries even before the pandemic, and there is a steep increase in mortality when primary percutaneous coronary intervention cannot be delivered in a timely fashion. Although the use of fibrinolytic therapy has progressively decreased over the last several decades in high-income countries, in circumstances when delays in timely delivery of primary percutaneous coronary intervention are expected, a modern fibrinolytic-based pharmacoinvasive strategy may need to be considered. The purpose of this review is to systematically discuss the contemporary role of an evidence-based fibrinolytic reperfusion strategy as part of a pharmacoinvasive approach, in the context of the emerging coronavirus disease 2019 pandemic.", "gemini": "Đại dịch bệnh do virus corona 2019 đang diễn ra đã tạo ra những thách thức bổ sung cho các hệ thống được thiết kế để thực hiện can thiệp động mạch vành qua da sơ cấp nhanh chóng cho những bệnh nhân có nhồi máu cơ tim đoạn ST chênh lên. Có 2 cân nhắc quan trọng: mục tiêu thời gian được khuyến cáo theo hướng dẫn rất khó đạt được đối với nhiều bệnh nhân ở các nước có thu nhập cao ngay cả trước khi xảy ra đại dịch và có sự gia tăng đột biến về tỷ lệ tử vong khi không thể tiến hành can thiệp động mạch vành qua da sơ cấp kịp thời. Mặc dù việc sử dụng liệu pháp tiêu sợi huyết đã giảm dần trong vài thập kỷ qua ở các nước có thu nhập cao, nhưng trong những trường hợp dự kiến sẽ chậm trễ trong việc cung cấp can thiệp động mạch vành qua da sơ cấp kịp thời, có thể cần xem xét một chiến lược dược lý xâm lấn dựa trên tiêu sợi huyết hiện đại. Mục đích của bài tổng quan này là thảo luận một cách có hệ thống về vai trò đương đại của một chiến lược tái tưới máu bằng thuốc tiêu sợi huyết dựa trên bằng chứng như một phần của phương pháp tiếp cận dược lý xâm lấn, trong bối cảnh đại dịch bệnh do virus corona 2019 mới nổi."} +{"idx": 1880026, "en": "During the last decade, non-vitamin K antagonist oral anticoagulants (NOACs) revolutionized the thromboprophylaxis management of several medical conditions, including atrial fibrillation and venous thromboembolism. Dabigatran etexilate was the first NOAC widely available worldwide, and it is currently the only one that directly inhibits thrombin. More recently, the availability of idarucizumab, a specific reversal agent, has increased the safety of dabigatran use in clinical practice, especially for those patients with severe and life-threatening bleeding. This review aims to summarize current evidence on dabigatran, starting from its pharmacological characteristics, and providing an updated overview of pivotal randomized controlled trials and real-world data on its efficacy and safety.", "gemini": "Trong thập kỷ qua, thuốc chống đông đường uống không phải đối kháng vitamin K (NOAC) đã cách mạng hóa việc quản lý dự phòng huyết khối của một số tình trạng bệnh lý, bao gồm rung nhĩ và thuyên tắc huyết khối tĩnh mạch. Dabigatran etexilate là NOAC đầu tiên có sẵn rộng rãi trên toàn thế giới và hiện là loại thuốc duy nhất ức chế trực tiếp thrombin. Gần đây hơn, sự ra đời của idarucizumab, một tác nhân đảo ngược đặc hiệu, đã làm tăng tính an toàn của việc sử dụng dabigatran trong thực hành lâm sàng, đặc biệt đối với những bệnh nhân bị chảy máu nghiêm trọng và đe dọa tính mạng. Mục tiêu của bài tổng quan này là tóm tắt các bằng chứng hiện tại về dabigatran, bắt đầu từ các đặc điểm dược lý của nó và cung cấp một bản tóm tắt cập nhật về các thử nghiệm đối chứng ngẫu nhiên quan trọng và dữ liệu thực tế về hiệu quả và tính an toàn của nó."} +{"idx": 1880027, "en": "Aim: Retrospectively assessed treatment patterns and clinical and economic outcomes in Merkel cell carcinoma (MCC) patients receiving recommended first-line regimens. Materials & methods: MCC patients newly treated with either immune checkpoint inhibitors (ICIs) or chemotherapies (CTs) were selected from the Veterans Health Administration database (2013-2018); 74 patients (ICIs: 20 and CTs: 54) were selected. Results: Median duration of therapy was 300 days for ICIs and 91 days for CTs. Time to next treatment was 245 and 184 days, respectively. Mean total (per patient per month) costs were $15,306 (ICIs) and $10,957 (CTs), of which 51% and 86%, respectively, were non-MCC therapy-related costs. Conclusion: Despite higher costs, utilization of ICIs in first-line MCC shows clinical advantages over CTs in the real world.", "gemini": "Mục đích: Đánh giá hồi cứu các phác đồ điều trị và kết quả lâm sàng và kinh tế ở những bệnh nhân bị ung thư tế bào Merkel (MCC) được dùng các phác đồ điều trị đầu tay được khuyến cáo. Vật liệu và phương pháp: Các bệnh nhân MCC mới được điều trị bằng thuốc ức chế điểm kiểm soát miễn dịch (ICI) hoặc hóa trị (CT) được chọn từ cơ sở dữ liệu của Cục Quản lý Y tế Cựu chiến binh (2013-2018); 74 bệnh nhân (ICI: 20 và CT: 54) đã được chọn. Kết quả: Thời gian điều trị trung bình là 300 ngày đối với ICI và 91 ngày đối với CT. Thời gian đến lần điều trị tiếp theo lần lượt là 245 và 184 ngày. Chi phí trung bình tổng cộng (trên mỗi bệnh nhân mỗi tháng) là 15.306 đô la (ICI) và 10.957 đô la (CT), trong đó lần lượt là 51% và 86% là chi phí không liên quan đến điều trị MCC. Kết luận: Mặc dù chi phí cao hơn, việc sử dụng ICI trong MCC đầu tay cho thấy lợi thế lâm sàng hơn CT trong thế giới thực."} +{"idx": 1880028, "en": "A pair of new isocoumarin derivative enantiomers, (S)-(-)-3-(3,3-dichloro-2-hydroxy-propyl)-4-chlorine-6,8-dihydroxy-isochromen-1- one (1a) and (R)-(+)-3-(3,3-dichloro-2-hydroxy-propyl)-4-chlorine-6,8-dihydroxy-isochromen-1- one (1 b), as well as seven known compounds (2-8) were isolated from Ludwigia hyssopifolia. Compounds 1a and 1 b were confirmed to be a pair of enantiomers by chiral HPLC-CD analysis, and the structure of compound 1 was determined by spectroscopic analyses including extensive 1 D (1H NMR, 13C NMR) and 2 D NMR spectra (COSY, HSQC and HMBC) and MS data. And the absolute configurations of compounds 1a and 1 b were determined by the quantum chemical ECD calculations. Compounds 2-8 are firstly reported from this plant. In the in vitro assays, compounds 5 and 8 can inhibit human laryngeal cancer Hep-2 cell line growth in a dose- and time-dependent manner. In addition, compounds 2 and 4 have effects on increasing glucose uptake in vitro. Compound 2 showed a strong glucose uptake in L6 cells, with enhancements by 1.8 folds.", "gemini": "Một cặp đồng phân quang học dẫn xuất isocoumarin mới, (S)-(-)-3-(3,3-dichloro-2-hydroxy-propyl)-4-chlorine-6,8-dihydroxy-isochromen-1-one (1a) và (R)-(+)-3-(3,3-dichloro-2-hydroxy-propyl)-4-chlorine-6,8-dihydroxy-isochromen-1-one (1b), cũng như bảy hợp chất đã biết (2-8) được phân lập từ Ludwigia hyssopifolia. Hợp chất 1a và 1b được xác nhận là một cặp đồng phân quang học bằng phương pháp phân tích HPLC-CD trên sắc ký lỏng, và cấu trúc của hợp chất 1 được xác định bằng phân tích quang phổ bao gồm phổ NMR 1D (1H NMR, 13C NMR) và phổ NMR 2D (COSY, HSQC và HMBC) và dữ liệu MS. Và cấu hình tuyệt đối của hợp chất 1a và 1b được xác định bằng các phép tính ECD hóa học lượng tử. Các hợp chất 2-8 được báo cáo lần đầu tiên từ loài thực vật này. Trong các xét nghiệm trong ống nghiệm, các hợp chất 5 và 8 có thể ức chế sự phát triển của dòng tế bào ung thư thanh quản ở người Hep-2 theo cách phụ thuộc vào liều lượng và thời gian. Ngoài ra, các hợp chất 2 và 4 có tác dụng làm tăng hấp thu glucose trong ống nghiệm. Hợp chất 2 cho thấy khả năng hấp thu glucose mạnh ở tế bào L6, với sự tăng cường gấp 1,8 lần."} +{"idx": 1880029, "en": "Baicalein exhibits anti-cancer roles in several cancers. However, the factors influencing the antitumorigenic efficiencies of baicalein in CC remain largely unclear. Here, we provide convincing evidences that lncRNA SNHG1 attenuates the tumor-suppressive roles of baicalein in CC cell viability, apoptosis, migration, and CC tumor growth. This study further demonstrates that the influences of SNHG1 in the antitumorigenic process of baicalein are achieved through modulating the miR-3127-5p/FZD4Wnt/β-catenin axis. SNHG1 attenuates the repressive role of baicalein on Wnt/β-catenin. Therefore, SNHG1 is a novel modulator of the tumor-suppressive roles of baicalein and SNHG1 represents a therapeutic intervention target to reinforce the tumor-suppressive roles of baicalein in CC.", "gemini": "Baicalein thể hiện vai trò chống ung thư trong một số loại ung thư. Tuy nhiên, các yếu tố ảnh hưởng đến hiệu quả chống ung thư của baicalein trong CC vẫn chưa được làm rõ. Ở đây, chúng tôi cung cấp bằng chứng thuyết phục rằng lncRNA SNHG1 làm giảm vai trò ức chế khối u của baicalein trong khả năng sống của tế bào CC, quá trình apoptosis, di chuyển và sự phát triển của khối u CC. Nghiên cứu này tiếp tục chứng minh rằng ảnh hưởng của SNHG1 trong quá trình chống ung thư của baicalein đạt được thông qua việc điều chỉnh trục miR-3127-5p/FZD4Wnt/β-catenin. SNHG1 làm giảm vai trò ức chế của baicalein đối với Wnt/β-catenin. Do đó, SNHG1 là một chất điều biến mới đối với vai trò ức chế khối u của baicalein và SNHG1 đại diện cho một mục tiêu can thiệp điều trị để củng cố vai trò ức chế khối u của baicalein trong CC."} +{"idx": 1880030, "en": "In eukaryotes three DNA polymerases (Pols), α, δ, and ε, are tasked with bulk DNA synthesis of nascent strands during genome duplication. Most evidence supports a model where Pol α initiates DNA synthesis before Pol ε and Pol δ replicate the leading and lagging strands, respectively. However, a number of recent reports, enabled by advances in biochemical and genetic techniques, have highlighted emerging roles for Pol δ in all stages of leading-strand synthesis; initiation, elongation, and termination, as well as fork restart. By focusing on these studies, this review provides an updated perspective on the division of labor between the replicative polymerases during DNA replication.", "gemini": "Ở eukaryote, ba DNA polymerase (Pols), α, δ và ε, có nhiệm vụ tổng hợp DNA số lượng lớn các sợi mới trong quá trình sao chép bộ gen. Hầu hết các bằng chứng đều ủng hộ mô hình Pol α khởi đầu tổng hợp DNA trước khi Pol ε và Pol δ sao chép lần lượt các sợi dẫn đầu và sợi chậm. Tuy nhiên, một số báo cáo gần đây, nhờ những tiến bộ trong các kỹ thuật sinh hóa và di truyền, đã nêu bật vai trò mới nổi của Pol δ trong tất cả các giai đoạn của quá trình tổng hợp sợi dẫn đầu: khởi đầu, kéo dài và kết thúc, cũng như khởi động lại chạc. Bằng cách tập trung vào các nghiên cứu này, bài đánh giá này cung cấp một góc nhìn cập nhật về sự phân công lao động giữa các polymerase sao chép trong quá trình sao chép DNA."} +{"idx": 1880031, "en": "Hematopoietic stem cell gene therapy has become a successful therapeutic strategy for some inherited genetic disorders. Pre-clinical toxicity studies performed to support the human clinical trials using viral-mediated gene transfer and autologous hematopoietic stem and progenitor cell (HSPC) transplantation are complex and the use of mouse models of human diseases makes interpretation of the results challenging. In addition, they rely on the use of conditioning agents that must induce enough myeloablation to allow engraftment of transduced and transplanted HSPC. Busulfan and total body irradiation (TBI) are the most commonly used conditioning regimens in the mouse. Lenticular degeneration and atrophy of reproductive organs are expected histopathological changes. Proliferative and nonproliferative lesions can be observed with different incidence and distribution across strains and mouse models of diseases. The occurrence of these lesions can interfere with the interpretation of pre-clinical toxicity and tumorigenicity studies performed to support the human clinical studies. As such, it is important to be aware of the background incidence of lesions induced by different conditioning regimens. We review the histopathology results from seven long-term studies, five using TBI and two using busulfan.", "gemini": "Liệu pháp gen tế bào gốc tạo máu đã trở thành một chiến lược điều trị thành công đối với một số rối loạn di truyền. Các nghiên cứu độc tính tiền lâm sàng được thực hiện để hỗ trợ các thử nghiệm lâm sàng trên người sử dụng phương pháp chuyển gen qua virus và ghép tế bào gốc tạo máu và tế bào tiền thân tự thân (HSPC) rất phức tạp và việc sử dụng mô hình chuột mắc bệnh ở người khiến việc giải thích kết quả trở nên khó khăn. Ngoài ra, chúng dựa vào việc sử dụng các tác nhân gây độc phải gây ra đủ tình trạng mất tủy để cho phép ghép HSPC đã chuyển gen và ghép. Busulfan và xạ trị toàn thân (TBI) là các chế độ gây độc phổ biến nhất ở chuột. Thoái hóa thấu kính và teo cơ quan sinh sản là những thay đổi mô bệnh học dự kiến. Có thể quan sát thấy các tổn thương tăng sinh và không tăng sinh với tỷ lệ mắc và phân bố khác nhau giữa các chủng và mô hình bệnh ở chuột. Sự xuất hiện của các tổn thương này có thể ảnh hưởng đến việc giải thích các nghiên cứu về độc tính tiền lâm sàng và khả năng gây ung thư được thực hiện để hỗ trợ các nghiên cứu lâm sàng trên người. Do đó, điều quan trọng là phải nhận thức được tỷ lệ mắc các tổn thương do các chế độ gây độc khác nhau gây ra. Chúng tôi xem xét kết quả mô bệnh học từ bảy nghiên cứu dài hạn, năm nghiên cứu sử dụng TBI và hai nghiên cứu sử dụng busulfan."} +{"idx": 1880032, "en": "Perceived social support is a known factor in preventing job burnout in many professions. However, the influence of coping strategies on perceived social support and job burnout remains unclear. The current study investigated firefighters using data from a two-wave study to fill this gap in knowledge. Chinese firefighters (N = 340) participated in the study for 3-months. Results indicated that perceived social support at baseline predicted job burnout at the 3-month follow-up through coping strategies. We concluded that perceived social support acts through positive coping strategies to reduce job burnout in Chinese firefighters.", "gemini": "Hỗ trợ xã hội được biết là một yếu tố trong việc ngăn ngừa kiệt sức công việc ở nhiều ngành nghề. Tuy nhiên, ảnh hưởng của các chiến lược đối phó đối với sự hỗ trợ xã hội được nhận thức và kiệt sức công việc vẫn chưa rõ ràng. Nghiên cứu hiện tại đã điều tra lực lượng cứu hỏa bằng cách sử dụng dữ liệu từ một nghiên cứu hai đợt để lấp đầy khoảng trống kiến thức này. Lực lượng cứu hỏa Trung Quốc (N = 340) đã tham gia nghiên cứu trong 3 tháng. Kết quả chỉ ra rằng sự hỗ trợ xã hội được nhận thức ở thời điểm ban đầu dự đoán tình trạng kiệt sức công việc sau 3 tháng theo dõi thông qua các chiến lược đối phó. Chúng tôi kết luận rằng sự hỗ trợ xã hội được nhận thức hoạt động thông qua các chiến lược đối phó tích cực để giảm kiệt sức công việc ở lực lượng cứu hỏa Trung Quốc."} +{"idx": 1880033, "en": "Trehalose and glycerol are low molecular mass sugars/polyols that have found widespread use in the protection of native protein states, in both short- and long-term storage of biological materials, and as a means of understanding protein dynamics. These myriad uses are often attributed to their ability to form an amorphous glassy matrix. In glycerol, the glass is formed only at cryogenic temperatures, while in trehalose, the glass is formed at room temperature, but only upon dehydration of the sample. While much work has been carried out to elucidate a mechanistic view of how each of these matrices interact with proteins to provide stability, rarely have the effects of these two independent systems been directly compared to each other. This review aims to compile decades of research on how different glassy matrices affect two types of photosynthetic proteins: (i) the Type II bacterial reaction center from Rhodobacter sphaeroides and (ii) the Type I Photosystem I reaction center from cyanobacteria. By comparing aggregate data on electron transfer, protein structure, and protein dynamics, it appears that the effects of these two distinct matrices are remarkably similar. Both seem to cause a \"tightening\" of the solvation shell when in a glassy state, resulting in severely restricted conformational mobility of the protein and associated water molecules. Thus, trehalose appears to be able to mimic, at room temperature, nearly all of the effects on protein dynamics observed in low temperature glycerol glasses.", "gemini": "Trehalose và glycerol là những loại đường/polyol có khối lượng phân tử thấp được sử dụng rộng rãi để bảo vệ trạng thái protein tự nhiên, trong cả quá trình bảo quản vật liệu sinh học trong thời gian ngắn và dài hạn, và như một phương tiện để hiểu động học protein. Muôn vàn công dụng này thường được quy cho khả năng tạo thành ma trận thủy tinh vô định hình của chúng. Trong glycerol, thủy tinh chỉ được hình thành ở nhiệt độ lạnh, trong khi trong trehalose, thủy tinh được hình thành ở nhiệt độ phòng, nhưng chỉ khi mẫu bị mất nước. Trong khi nhiều công trình đã được thực hiện để làm sáng tỏ quan điểm cơ học về cách mỗi ma trận này tương tác với protein để cung cấp sự ổn định, thì hiếm khi nào tác động của hai hệ thống độc lập này được so sánh trực tiếp với nhau. Mục tiêu của bài đánh giá này là biên soạn hàng thập kỷ nghiên cứu về cách các ma trận thủy tinh khác nhau ảnh hưởng đến hai loại protein quang hợp: (i) trung tâm phản ứng của vi khuẩn Loại II từ Rhodobacter sphaeroides và (ii) trung tâm phản ứng của quang hệ I Loại I từ vi khuẩn lam. Bằng cách so sánh dữ liệu tổng hợp về truyền electron, cấu trúc protein và động học protein, có vẻ như tác động của hai ma trận riêng biệt này rất giống nhau. Cả hai dường như đều gây ra hiện tượng \"thắt chặt\" lớp solvat hóa khi ở trạng thái thủy tinh, dẫn đến hạn chế nghiêm trọng tính linh động về mặt cấu hình của protein và các phân tử nước liên quan. Do đó, trehalose dường như có thể bắt chước, ở nhiệt độ phòng, hầu hết mọi tác động lên động học protein quan sát thấy trong thủy tinh glycerol ở nhiệt độ thấp."} +{"idx": 1880034, "en": "The sinus tract of the ischial tuberosity is often caused by pressure injury. It has the characteristics of difficult treatment and high cost, which increases the anxiety of patients and reduces the quality of life of patients. This case report is to describe an effective method to treat sinus wound at the ischial tuberosity. A 53-year-old male suffered pressure ulcer with sinus wound at the left ischial tuberosity due to inadequate walking and sedentary activity. On the basis of pressure relief and immobilization, the patient was treated with CO2 laser debridement and negative pressure wound therapy under endoscope support 3 times, the deep of the sinus wound was completely closed, and then the residual superficial wound was treated by skin grafting. Follow-up of 1 year after healing showed no recurrence of wound.", "gemini": "Ống xoang của củ ngồi thường do chấn thương do áp lực. Nó có đặc điểm là khó điều trị và tốn kém, làm tăng sự lo lắng của bệnh nhân và làm giảm chất lượng cuộc sống của bệnh nhân. Báo cáo trường hợp này là để mô tả một phương pháp hiệu quả để điều trị vết thương xoang ở củ ngồi. Một nam giới 53 tuổi bị loét do tỳ đè với vết thương xoang ở củ ngồi trái do đi lại không đúng cách và ít vận động. Trên cơ sở giảm áp và bất động, bệnh nhân đã được điều trị bằng phương pháp cắt lọc bằng laser CO2 và liệu pháp áp lực âm dưới sự hỗ trợ của nội soi 3 lần, phần sâu của vết thương xoang đã được đóng hoàn toàn, sau đó vết thương nông còn lại được điều trị bằng ghép da. Theo dõi 1 năm sau khi lành không thấy vết thương tái phát."} +{"idx": 1880035, "en": "This is the first study to assess the impact of social egg freezing (SEF) on quality of life. This cross-sectional survey utilised the FertiQoL treatment module in women who underwent SEF between January 2008 and October 2019 (n = 94). The mean treatment score was 65.5, whereas the tolerability and environment scores were 62.4 and 68 respectively. Being married or in a relationship was associated with higher, albeit not statistically significant, scores (69.2) when compared to single women (66.3; p = 0.49). However, being separated or divorced was associated with significantly worse scores compared to married women, women in a relationship and single women (43.3 vs. 67.0; p = 0.001). There were no significant differences in scores between younger and older women, low and high number of oocytes stored, duration of stimulation cycles, or in those who had single or multiple cycles. These data suggest SEF is largely tolerable, with favourable FertiQoL scores compared to infertile women undergoing IVF. However, whilst such women are physiologically fertile, their situation renders them socially infertile. As such, women undergoing SEF should be identified as a population that requires additional support, who should be offered extensive counselling, active monitoring throughout the process and additional support if required.", "gemini": "Đây là nghiên cứu đầu tiên đánh giá tác động của việc đông lạnh trứng xã hội (SEF) đối với chất lượng cuộc sống. Khảo sát cắt ngang này sử dụng mô-đun điều trị FertiQoL ở những phụ nữ đã trải qua SEF trong khoảng thời gian từ tháng 1 năm 2008 đến tháng 10 năm 2019 (n = 94). Điểm điều trị trung bình là 65,5, trong khi điểm dung nạp và môi trường lần lượt là 62,4 và 68. Kết hôn hoặc có mối quan hệ được liên kết với số điểm cao hơn, mặc dù không có ý nghĩa thống kê (69,2) khi so sánh với những phụ nữ độc thân (66,3; p = 0,49). Tuy nhiên, việc ly thân hoặc ly hôn có liên quan đến số điểm tệ hơn đáng kể so với phụ nữ đã kết hôn, phụ nữ có mối quan hệ và phụ nữ độc thân (43,3 so với 67,0; p = 0,001). Không có sự khác biệt đáng kể về điểm số giữa phụ nữ trẻ và lớn tuổi, số lượng nang trứng được lưu trữ ít và nhiều, thời gian chu kỳ kích thích hoặc ở những phụ nữ có chu kỳ đơn hoặc nhiều chu kỳ. Những dữ liệu này cho thấy SEF phần lớn có thể dung nạp được, với điểm số FertiQoL thuận lợi so với những phụ nữ vô sinh đang thực hiện IVF. Tuy nhiên, trong khi những phụ nữ như vậy có khả năng sinh sản về mặt sinh lý, thì tình trạng của họ khiến họ trở nên vô sinh về mặt xã hội. Do đó, những phụ nữ đang trải qua SEF nên được xác định là đối tượng cần được hỗ trợ thêm, những người nên được cung cấp dịch vụ tư vấn mở rộng, theo dõi tích cực trong suốt quá trình và hỗ trợ thêm nếu cần."} +{"idx": 1880036, "en": "OBJECTIVES To investigate the prevalence of self-reported pain-related temporomandibular disorders (TMDs) and its association with psychological status in a dental clinic setting. METHODS In this cross-sectional study, patients were asked to complete a TMD pain screener and the Patient Health Questionnaire-4 (PHQ-4). Correlations between symptoms of pain-related TMDs and PHQ-4 scores were analysed using Spearman's correlation test. Symptoms of pain-related TMDs were compared between four groups of participants with different psychological profiles using the Kruskal-Wallis test followed by multiple comparisons. The level of significance was adjusted using the Dunn-Bonferroni test. RESULTS The prevalence of self-reported pain-related TMDs was 22.2%. TMD pain score was positively correlated with PHQ-4 score. The high anxiety and the comorbidity groups had significantly higher TMD pain scores than the controls. CONCLUSION There was a high prevalence of self-reported pain-related TMDs, which was correlated with scores on all psychological assessment scales. Symptoms of pain-related TMDs were significantly greater in patients with high anxiety scores, regardless of depression level.", "gemini": "MỤC TIÊU Nghiên cứu tỷ lệ rối loạn khớp thái dương hàm (TMD) liên quan đến đau tự báo cáo và mối liên quan của nó với tình trạng tâm lý trong bối cảnh phòng khám nha khoa. PHƯƠNG PHÁP Trong nghiên cứu cắt ngang này, bệnh nhân được yêu cầu hoàn thành công cụ sàng lọc đau TMD và Bản câu hỏi sức khỏe bệnh nhân-4 (PHQ-4). Các mối tương quan giữa các triệu chứng của TMD liên quan đến đau và điểm PHQ-4 được phân tích bằng kiểm tra tương quan của Spearman. Các triệu chứng của TMD liên quan đến đau được so sánh giữa bốn nhóm người tham gia có hồ sơ tâm lý khác nhau bằng cách sử dụng kiểm tra Kruskal-Wallis tiếp theo là nhiều so sánh. Mức độ ý nghĩa được điều chỉnh bằng cách sử dụng kiểm tra Dunn-Bonferroni. KẾT QUẢ Tỷ lệ TMD liên quan đến đau tự báo cáo là 22,2%. Điểm đau TMD tương quan thuận với điểm PHQ-4. Nhóm lo âu cao và nhóm bệnh đi kèm có điểm đau TMD cao hơn đáng kể so với nhóm đối chứng. KẾT LUẬN Có tỷ lệ TMD liên quan đến đau tự báo cáo cao, có tương quan với điểm số trên tất cả các thang đánh giá tâm lý. Các triệu chứng của TMD liên quan đến đau lớn hơn đáng kể ở những bệnh nhân có điểm lo âu cao, bất kể mức độ trầm cảm."} +{"idx": 1880037, "en": "OBJECTIVE There have been few studies on hematological parameters (e.g., hemoglobin, red cell distribution width, white blood cells, and mean platelet volume), and polycystic ovary syndrome (PCOS). This study aimed to compare hematological parameters between women with PCOS and controls. METHODS We performed an age-matched case-control study in Faisal bin Mishal Center for Infertility, Buraidah (Kingdom of Saudi Arabia). The cases were women with PCOS and an equal number of healthy women were enrolled as controls. The basic gynecological history was recorded and blood samples were analyzed for blood parameters using an automated hematology analyzer. RESULTS The two groups (60 women in each arm of the study) were similar in age. However, body mass index was significantly higher in women with PCOs compared with controls. There were no significant differences in any of the hematological parameters (hemoglobin, red blood cells, red cell distribution width, white blood cells, platelets, and mean platelet volume) between the two groups. CONCLUSION There does not appear to be a significant difference in hematological parameters in Saudi women with PCOS and healthy controls. A larger study on this issue is required in the future.", "gemini": "MỤC TIÊU Có rất ít nghiên cứu về các thông số huyết học (ví dụ: hemoglobin, độ rộng phân bố hồng cầu, bạch cầu và thể tích tiểu cầu trung bình) và hội chứng buồng trứng đa nang (PCOS). Nghiên cứu này nhằm mục đích so sánh các thông số huyết học giữa phụ nữ mắc PCOS và nhóm đối chứng. PHƯƠNG PHÁP Chúng tôi đã thực hiện một nghiên cứu đối chứng theo ca ghép đôi theo độ tuổi tại Trung tâm vô sinh Faisal bin Mishal, Buraidah (Vương quốc Ả Rập Saudi). Các trường hợp là những phụ nữ mắc PCOS và một số lượng phụ nữ khỏe mạnh tương đương được ghi danh làm nhóm đối chứng. Tiền sử phụ khoa cơ bản được ghi lại và các mẫu máu được phân tích các thông số máu bằng máy phân tích huyết học tự động. KẾT QUẢ Hai nhóm (60 phụ nữ ở mỗi nhánh của nghiên cứu) có độ tuổi tương tự nhau. Tuy nhiên, chỉ số khối cơ thể cao hơn đáng kể ở những phụ nữ mắc PCOS so với nhóm đối chứng. Không có sự khác biệt đáng kể nào về bất kỳ thông số huyết học nào (hemoglobin, hồng cầu, độ rộng phân bố hồng cầu, bạch cầu, tiểu cầu và thể tích tiểu cầu trung bình) giữa hai nhóm. KẾT LUẬN Có vẻ như không có sự khác biệt đáng kể về các thông số huyết học ở phụ nữ Saudi mắc PCOS và nhóm đối chứng khỏe mạnh. Cần một nghiên cứu lớn hơn về vấn đề này trong tương lai."} +{"idx": 1880038, "en": "The aim of the study was to examine the clinical value of blastocysts derived from mono-pronuclear (1PN) or non-pronuclear (0PN) zygotes with two polar bodies (2PB), which were selected by our criteria. We retrospectively analysed 610 frozen-thawed blastocyst transfer (FET) cycles and the corresponding oocyte retrieval cycles from 2014 to 2017. Developmental potential and clinical outcomes of embryos derived from zygotes with various numbers of pronuclei were analysed. Based on more detailed pre-selection settings, blastulation rates of 1PN/2PB and 0PN/2PB-derived embryos were 70.18% and 69.17%, respectively. Blastocyst FET results were not significantly different between 2PN/2PB, 1PN/2PB and 0PN/2PB groups in terms of clinical pregnancy rates (59.79%, 47.06% and 56.25%), implantation rates (47.24%, 40.00% and 47.62%), live birth rates (49.39%, 29.41% and 43.75%) or malformation rates (0%, 0% and 0%). In conclusion, after strict morphological selection and blastocyst culture, 1PN/2PB and 0PN/2PB-derived embryos in IVF cycles can have considerable clinical value. Blastocysts derived from 1PN/2PB or 0PN/2PB zygotes are worthwhile FET option for patients who have no available 2PN-derived embryos.", "gemini": "Mục đích của nghiên cứu là kiểm tra giá trị lâm sàng của phôi nang có nguồn gốc từ hợp tử đơn nhân (1PN) hoặc không nhân (0PN) có hai thể cực (2PB), được lựa chọn theo tiêu chuẩn của chúng tôi. Chúng tôi đã phân tích hồi cứu 610 chu kỳ chuyển phôi nang đông lạnh-rã đông (FET) và các chu kỳ lấy noãn tương ứng từ năm 2014 đến 2017. Khả năng phát triển và kết quả lâm sàng của phôi có nguồn gốc từ hợp tử có nhiều nhân khác nhau đã được phân tích. Dựa trên các thiết lập lựa chọn trước chi tiết hơn, tỷ lệ phôi nang của phôi có nguồn gốc từ 1PN / 2PB và 0PN / 2PB lần lượt là 70,18% và 69,17%. Kết quả FET phôi nang không có sự khác biệt đáng kể giữa các nhóm 2PN / 2PB, 1PN / 2PB và 0PN / 2PB về tỷ lệ có thai lâm sàng (59,79%, 47,06% và 56,25%), tỷ lệ làm tổ (47,24%, 40,00% và 47,62%), tỷ lệ sinh con sống (49,39%, 29,41% và 43,75%) hoặc tỷ lệ dị tật (0%, 0% và 0%). Tóm lại, sau khi lựa chọn hình thái nghiêm ngặt và nuôi cấy phôi nang, phôi có nguồn gốc từ 1PN / 2PB và 0PN / 2PB trong các chu kỳ IVF có thể có giá trị lâm sàng đáng kể. Phôi nang có nguồn gốc từ hợp tử 1PN / 2PB hoặc 0PN / 2PB là lựa chọn FET đáng giá cho những bệnh nhân không có phôi có nguồn gốc từ 2PN."} +{"idx": 1880039, "en": "INTRODUCTION Treatment of Behçet's syndrome (BS) is aimed at controlling all symptoms of such a complex disorder, ensuring a good quality of life and preventing life-threatening complications. A better understanding of the pathogenic role of different chemokines has improved our knowledge of BS and elicited a more specific use of therapies currently available, minimizing the burden of potential side-effects related to treatment. AREAS COVERED This work aims to provide a detailed overview of the safety profile for current therapies available in the treatment of BS, focusing on the main side-effects, toxicity and contraindications. EXPERT OPINION The greatest experience in the management of BS has been achieved with the employment of monoclonal anti-tumor necrosis factor antibodies which have been advocated for BS refractory manifestations. Moreover, interleukin-1 inhibitors have proven to be effective as well as safe, despite escalation of their dosage, especially to manage the most severe and difficult-to-treat ocular manifestations. However, general treatment of BS patients remains awkward as protean clinical features may respond differently to the same treatment or even worsen. Therefore, patients' safety for therapies used in BS promotes the implementation of precision medicine, which could help targeting accurately the pathogenetic mechanisms concealed behind specific clinical phenotypes.", "gemini": "GIỚI THIỆU Mục tiêu điều trị hội chứng Behçet (BS) là kiểm soát mọi triệu chứng của một rối loạn phức tạp như vậy, đảm bảo chất lượng cuộc sống tốt và ngăn ngừa các biến chứng đe dọa tính mạng. Việc hiểu rõ hơn về vai trò gây bệnh của các chemokine khác nhau đã cải thiện kiến thức của chúng ta về BS và đưa ra cách sử dụng cụ thể hơn các liệu pháp hiện có, giảm thiểu gánh nặng của các tác dụng phụ tiềm ẩn liên quan đến điều trị. CÁC LĨNH VỰC ĐƯỢC BAO PHỦ Công trình này nhằm cung cấp một cái nhìn tổng quan chi tiết về hồ sơ an toàn cho các liệu pháp hiện có trong điều trị BS, tập trung vào các tác dụng phụ chính, độc tính và chống chỉ định. Ý KIẾN CHUYÊN GIA Kinh nghiệm lớn nhất trong việc quản lý BS đã đạt được với việc sử dụng các kháng thể đơn dòng chống yếu tố hoại tử khối u đã được ủng hộ cho các biểu hiện khó chữa của BS. Hơn nữa, các chất ức chế interleukin-1 đã được chứng minh là có hiệu quả cũng như an toàn, mặc dù liều lượng của chúng tăng lên, đặc biệt là để kiểm soát các biểu hiện về mắt nghiêm trọng nhất và khó điều trị nhất. Tuy nhiên, việc điều trị chung cho bệnh nhân BS vẫn còn khó khăn vì các đặc điểm lâm sàng có thể đáp ứng khác nhau với cùng một phương pháp điều trị hoặc thậm chí trở nên tồi tệ hơn. Do đó, sự an toàn của bệnh nhân đối với các liệu pháp được sử dụng trong BS thúc đẩy việc triển khai y học chính xác, có thể giúp nhắm mục tiêu chính xác vào các cơ chế sinh bệnh ẩn đằng sau các kiểu hình lâm sàng cụ thể."} +{"idx": 1880040, "en": "BACKGROUND Given the range of subjective experiences reported by patients with chronic pain, Spinal Cord Stimulation (SCS) systems designed for tailored delivery of analgesic therapy may help improve treatment effectiveness and satisfaction. RESEARCH DESIGN AND METHODS This case-series evaluated 420 patients with chronic back and/or leg pain implanted with an SCS device capable of sequential or simultaneous delivery of neurostimulation (i.e. combination therapy) as well as multiple waveforms and/or field shapes. Following implantation, an array of standard programs (e.g. paresthesia-based SCS), and a custom set of sub-perception programs were provided per patient feedback. Pain scores (Numeric Rating Scale, NRS) were collected at baseline and during follow-up. RESULTS In this multicenter, observational series (n = 420, 53.1% female; Age: 64.2 ± 13.4 years), a mean overall pain score of 7.2 ± 1.8 (SD) was reported pre-trial (Baseline). At a mean follow-up duration of 208 ± 200 (SD) days, the mean overall pain score reduced to 2.4 (p < 0.0001). Overall pain was reduced by 5.1 ± 2.4 and 4.5 ± 2.4 points (NRS) at 3-months (N = 256) and at 12-months post-implant (N = 122) respectively (p < 0.0001). CONCLUSIONS These results suggest that highly 'customizable' SCS approaches may allow for highly effective pain relief within the real-world clinical setting.", "gemini": "BỐI CẢNH Xét đến phạm vi các trải nghiệm chủ quan được báo cáo bởi những bệnh nhân bị đau mãn tính, các hệ thống kích thích tủy sống (SCS) được thiết kế để cung cấp liệu pháp giảm đau theo yêu cầu có thể giúp cải thiện hiệu quả điều trị và sự hài lòng. THIẾT KẾ NGHIÊN CỨU VÀ PHƯƠNG PHÁP Loạt trường hợp này đã đánh giá 420 bệnh nhân bị đau lưng và/hoặc đau chân mãn tính được cấy ghép thiết bị SCS có khả năng cung cấp kích thích thần kinh tuần tự hoặc đồng thời (tức là liệu pháp kết hợp) cũng như nhiều dạng sóng và/hoặc hình dạng trường. Sau khi cấy ghép, một loạt các chương trình tiêu chuẩn (ví dụ: SCS dựa trên cảm giác dị cảm) và một bộ các chương trình nhận thức phụ tùy chỉnh đã được cung cấp theo phản hồi của bệnh nhân. Điểm đau (Thang đánh giá số, NRS) được thu thập lúc ban đầu và trong quá trình theo dõi. KẾT QUẢ Trong loạt quan sát đa trung tâm này (n = 420, 53,1% là nữ; Tuổi: 64,2 ± 13,4 tuổi), điểm đau trung bình chung là 7,2 ± 1,8 (SD) được báo cáo trước thử nghiệm (Mức cơ sở). Ở thời điểm theo dõi trung bình là 208 ± 200 (SD) ngày, điểm đau trung bình chung giảm xuống còn 2,4 (p < 0,0001). Đau tổng thể giảm 5,1 ± 2,4 và 4,5 ± 2,4 điểm (NRS) ở thời điểm 3 tháng (N = 256) và ở thời điểm 12 tháng sau khi cấy ghép (N = 122) tương ứng (p < 0,0001). KẾT LUẬN Những kết quả này cho thấy rằng các phương pháp tiếp cận SCS 'có thể tùy chỉnh' cao có thể giúp giảm đau hiệu quả cao trong bối cảnh lâm sàng thực tế."} +{"idx": 1880041, "en": "BACKGROUND Patients suffering from postoperative recurrent glioblastoma have an extremely unfavorable outcome because there are no proven therapeutic options. The median overall survival for those with relapsed glioblastoma after surgery is only 7.5 months.Case presentation: Between March 2015 and October 2019, a 44-year-old female patient with recurrent glioblastoma was treated by our medical team. After several failed rounds of therapy, the patient was subsequently treated with the anti-programmed death (PD)-1 antibody nivolumab, anti-vascular endothelial growth factor (VEGF) antibody bevacizumab, and cytotoxic agent temozolomide. RESULTS The patient showed a sustainable complete response to the regimen. To date, there have been no serious toxic side effects. As of October 2019 (the last follow-up), the patient has been in complete remission for 17 months since recurrence. CONCLUSION The experience of this complicated case indicates the possible application of immune checkpoint inhibitors, anti-angiogenesis agents, and cytotoxic reagents for recurrent glioblastoma. The administration of this three-agent regimen appears safe and effective. However, further clinical trials are warranted.", "gemini": "BỐI CẢNH Những bệnh nhân bị u nguyên bào thần kinh đệm tái phát sau phẫu thuật có kết quả cực kỳ không thuận lợi vì không có lựa chọn điều trị nào được chứng minh. Tỷ lệ sống chung trung bình của những người bị u nguyên bào thần kinh đệm tái phát sau phẫu thuật chỉ là 7,5 tháng. Báo cáo trường hợp: Từ tháng 3 năm 2015 đến tháng 10 năm 2019, một bệnh nhân nữ 44 tuổi bị u nguyên bào thần kinh đệm tái phát đã được nhóm y tế của chúng tôi điều trị. Sau nhiều đợt điều trị không thành công, bệnh nhân sau đó được điều trị bằng kháng thể nivolumab chống chết theo chương trình (PD) -1, kháng thể chống yếu tố tăng trưởng nội mạc mạch máu (VEGF) bevacizumab và chất độc tế bào temozolomide. KẾT QUẢ Bệnh nhân cho thấy đáp ứng hoàn toàn bền vững với phác đồ điều trị. Cho đến nay, chưa có tác dụng phụ độc hại nghiêm trọng nào. Tính đến tháng 10 năm 2019 (lần theo dõi cuối cùng), bệnh nhân đã thuyên giảm hoàn toàn trong 17 tháng kể từ khi tái phát. KẾT LUẬN Kinh nghiệm của trường hợp phức tạp này cho thấy khả năng ứng dụng của thuốc ức chế điểm kiểm soát miễn dịch, thuốc chống tân sinh mạch và thuốc độc tế bào đối với u nguyên bào thần kinh đệm tái phát. Việc thực hiện phác đồ ba tác nhân này có vẻ an toàn và hiệu quả. Tuy nhiên, cần tiến hành thêm các thử nghiệm lâm sàng."} +{"idx": 1880042, "en": "CONTEXT Despite the abundance of knowledge regarding high-altitude pulmonary edoema (HAPE) and high-altitude pulmonary hypertension (HAPH), their prevalence continues to be on the rise. Thus, there is an urgent need for newer safe, effective, and relatively economic drug candidates. China is particularly known for the use of medicinal plants. OBJECTIVE This review summarizes the medicinal plants used for HAPE and HAPH in the past 30 years, as well as some potential plants. METHODS Publications on HAPE and HAPH from 1990 to 2020 were identified using Web of Science, PubMed, SCOPUS, Springer Link, Google Scholar databases, Chinese Clinical Trial Registry and CNKI with the following keywords: 'medicinal plants,' 'hypoxia,' 'high altitude pulmonary edema,' 'high altitude pulmonary hypertension,' 'pathophysiology,' 'mechanisms,' 'prevention,' 'treatment,' 'human,' 'clinical,' 'safety,' and 'pharmacokinetics.' RESULTS We found 26 species (from 20 families) out of 5000 plants which are used for HAPE and HAPH prevention or treatment. Rhodiola rosea Linn. (Crassulaceae) is the most widely utilized. The most involved family is Lamiaceae, which contains 5 species. DISCUSSION AND CONCLUSIONS We mainly reviewed the medicinal plants and mechanisms for the treatment of HAPE and HAPH, and we also assessed related toxicology experiments, pharmacokinetics and bioavailability. Potential medicinal plants were also identified. Further research is needed to determine the pharmacological effects and active ingredients of these potential medicinal plants.", "gemini": "BỐI CẢNH Mặc dù có nhiều kiến thức về phù phổi do độ cao (HAPE) và tăng huyết áp phổi do độ cao (HAPH), tỷ lệ mắc bệnh của chúng vẫn tiếp tục gia tăng. Do đó, có nhu cầu cấp thiết đối với các ứng cử viên thuốc mới an toàn, hiệu quả và tương đối kinh tế. Trung Quốc đặc biệt nổi tiếng với việc sử dụng các loại cây thuốc. MỤC TIÊU Bài tổng quan này tóm tắt các loại cây thuốc được sử dụng cho HAPE và HAPH trong 30 năm qua, cũng như một số loại cây tiềm năng. PHƯƠNG PHÁP Các ấn phẩm về HAPE và HAPH từ năm 1990 đến năm 2020 đã được xác định bằng cách sử dụng các cơ sở dữ liệu Web of Science, PubMed, SCOPUS, Springer Link, Google Scholar, Chinese Clinical Trial Registry và CNKI với các từ khóa sau: 'cây thuốc', 'thiếu oxy', 'phù phổi do độ cao', 'tăng huyết áp phổi do độ cao', 'bệnh sinh', 'cơ chế', 'phòng ngừa', 'điều trị', 'con người', 'lâm sàng', 'an toàn' và 'dược động học'. KẾT QUẢ Chúng tôi đã tìm thấy 26 loài (thuộc 20 họ) trong số 5000 loài thực vật được sử dụng để phòng ngừa hoặc điều trị HAPE và HAPH. Rhodiola rosea Linn. (Crassulaceae) là loài được sử dụng rộng rãi nhất. Họ liên quan nhiều nhất là Lamiaceae, chứa 5 loài. THẢO LUẬN VÀ KẾT LUẬN Chúng tôi chủ yếu xem xét các loại cây thuốc và cơ chế điều trị HAPE và HAPH, đồng thời chúng tôi cũng đánh giá các thí nghiệm độc tính liên quan, dược động học và sinh khả dụng. Các loại cây thuốc tiềm năng cũng đã được xác định. Cần nghiên cứu thêm để xác định các tác dụng dược lý và thành phần hoạt tính của các loại cây thuốc tiềm năng này."} +{"idx": 1880043, "en": "Mastocytosis is an accumulation of clonal mast cells within tissues and it is most commonly caused by an activating mutation in the KIT gene. In this study, we report a neonatal case who presented with diffuse cutaneous mastocytosis (CM) at birth. In China, nine other cases of neonatal-onset CM have been reported in the literature since 2006. In those cases, diffuse CM and urticaria pigmentosa were the main symptoms, and mutations in exon 17 at codon 816 in KIT were identified.", "gemini": "Bệnh mastocytosis là sự tích tụ của tế bào mast vô tính trong các mô và thường do đột biến hoạt hóa trong gen KIT gây ra. Trong nghiên cứu này, chúng tôi báo cáo một trường hợp sơ sinh biểu hiện bệnh mastocytosis da lan tỏa (CM) khi mới sinh. Tại Trung Quốc, chín trường hợp khác về CM khởi phát ở trẻ sơ sinh đã được báo cáo trong tài liệu kể từ năm 2006. Trong những trường hợp đó, CM lan tỏa và mề đay mảng là các triệu chứng chính và các đột biến ở exon 17 tại codon 816 trong KIT đã được xác định."} +{"idx": 1880044, "en": "BACKGROUND The Affordable Care Act (ACA) is one of the biggest healthcare reforms in US history. A key issue is the ACAs effect on low acuity, potentially primary care patients. This study evaluates the effect of the ACA on low acuity patients seen in the emergency department (ED). METHODS This is an age-period-cohort analysis for a community hospital ED in Michigan, from 2009 to 2015. Patients were stratified by age, year seen, emergency severity index (ESI) and insurance status. Data were compared between before and after ACA along with descriptive statistics, Chi-square and Student t-tests. The primary outcome was the change in ED usage by low acuity. Patients > 65 were used as a temporal control. RESULTS 305,350 ED visits were analyzed. ED visits with ESI 4/5 increased from 11.9% to 14.8%. Patients < 19 years increased from 25.5% to 34.3% (p = .0026). Ages 19-25 increased from 16.3% to 19.7% (p = 0.0515). Ages 26-64 increased from 11% to 14.9% (p = 0.0129). Ages > 65 increased from 5.1% to 6.5%. Patients < 65 showed a decreased uninsured rate from 12.30% to 6.28% (p < 0.0001). Comparatively, for age > 65: uninsured rate remained the same 0.46% to 0.49%. CONCLUSION Low acuity ED visits increased with the ACA reform in conjunction with a more insured population.", "gemini": "BỐI CẢNH Đạo luật chăm sóc giá cả phải chăng (ACA) là một trong những cải cách chăm sóc sức khỏe lớn nhất trong lịch sử Hoa Kỳ. Một vấn đề chính là tác động của ACA đối với các bệnh nhân có khả năng chăm sóc chính, cấp độ thấp. Nghiên cứu này đánh giá tác động của ACA đối với những bệnh nhân cấp độ thấp được khám tại khoa cấp cứu (ED). PHƯƠNG PHÁP Đây là phân tích theo giai đoạn tuổi cho khoa cấp cứu của bệnh viện cộng đồng ở Michigan, từ năm 2009 đến năm 2015. Bệnh nhân được phân tầng theo độ tuổi, năm khám, chỉ số mức độ khẩn cấp (ESI) và tình trạng bảo hiểm. Dữ liệu được so sánh giữa trước và sau ACA cùng với thống kê mô tả, Chi-square và kiểm định t của Student. Kết quả chính là sự thay đổi trong việc sử dụng ED ở mức độ cấp độ thấp. Bệnh nhân> 65 được sử dụng làm đối chứng thời gian. KẾT QUẢ 305.350 lượt khám ED đã được phân tích. Các lượt khám ED có ESI 4/5 tăng từ 11,9% lên 14,8%. Bệnh nhân <19 tuổi tăng từ 25,5% lên 34,3% (p = 0,0026). Độ tuổi từ 19-25 tăng từ 16,3% lên 19,7% (p = 0,0515). Độ tuổi từ 26-64 tăng từ 11% lên 14,9% (p = 0,0129). Độ tuổi> 65 tăng từ 5,1% lên 6,5%. Bệnh nhân <65 tuổi cho thấy tỷ lệ không có bảo hiểm giảm từ 12,30% xuống 6,28% (p <0,0001). So sánh, đối với độ tuổi> 65: tỷ lệ không có bảo hiểm vẫn giữ nguyên từ 0,46% đến 0,49%. KẾT LUẬN Các lượt khám cấp cứu cấp độ thấp đã tăng lên cùng với cải cách ACA cùng với dân số được bảo hiểm nhiều hơn."} +{"idx": 1880045, "en": "Employees are a very important source of innovation and essential for the generation, dissemination and implementation of these ideas throughout the organization. This is especially relevant when considering innovation in services during service (co-) creation such as within the healthcare sector. However, perceived employee involvement in innovation (EII) and between stakeholder group interactions in hospitals has not yet been studied in detail. This paper addresses the following research questions: \"How do different employee groups perceive their involvement in the innovation process in hospitals and how do their actual involvement levels differ?\" and (2) \"How do different employee groups perceive their interaction with other employee groups in the innovation process and how do their actual interactions differ?\" We analyzed a single typical German research hospital and conducted episodic interviews with employees representing different staff groups. We revealed that while all groups of employees are involved in innovation activities, perception of their involvement in innovation activities differs widely. There is a gap between perception and actual involvement particularly for lower level employees such as nurses. Further, their interaction differs among employee groups and innovation takes place in-group, rather than through group interaction. With our paper, we add to the understanding of perceived EII in hospitals and discuss measures for hospital management to increase EII.", "gemini": "Nhân viên là nguồn đổi mới rất quan trọng và thiết yếu cho việc tạo ra, phổ biến và thực hiện những ý tưởng này trong toàn bộ tổ chức. Điều này đặc biệt có liên quan khi xem xét đổi mới trong các dịch vụ trong quá trình (đồng) sáng tạo dịch vụ như trong lĩnh vực chăm sóc sức khỏe. Tuy nhiên, sự tham gia của nhân viên được nhận thức trong đổi mới (EII) và giữa các tương tác của nhóm bên liên quan trong bệnh viện vẫn chưa được nghiên cứu chi tiết. Bài báo này giải quyết các câu hỏi nghiên cứu sau: \"Các nhóm nhân viên khác nhau nhận thức như thế nào về sự tham gia của họ vào quá trình đổi mới trong bệnh viện và mức độ tham gia thực tế của họ khác nhau như thế nào?\" và (2) \"Các nhóm nhân viên khác nhau nhận thức như thế nào về sự tương tác của họ với các nhóm nhân viên khác trong quá trình đổi mới và sự tương tác thực tế của họ khác nhau như thế nào?\" Chúng tôi đã phân tích một bệnh viện nghiên cứu điển hình của Đức và tiến hành các cuộc phỏng vấn theo từng giai đoạn với các nhân viên đại diện cho các nhóm nhân viên khác nhau. Chúng tôi tiết lộ rằng trong khi tất cả các nhóm nhân viên đều tham gia vào các hoạt động đổi mới, thì nhận thức của họ về sự tham gia của họ vào các hoạt động đổi mới lại khác nhau rất nhiều. Có một khoảng cách giữa nhận thức và sự tham gia thực tế, đặc biệt là đối với những nhân viên cấp thấp như y tá. Hơn nữa, sự tương tác của họ khác nhau giữa các nhóm nhân viên và sự đổi mới diễn ra trong nhóm, chứ không phải thông qua tương tác nhóm. Với bài báo của mình, chúng tôi bổ sung thêm vào sự hiểu biết về EII được nhận thức trong các bệnh viện và thảo luận các biện pháp để ban quản lý bệnh viện tăng EII."} +{"idx": 1880046, "en": "Twelfth rib syndrome is a rare condition that causes severe pain in the loin. The diagnosis of this phenomenon is based on the patient's medical history and physical examination findings. However, many clinicians still lack an understanding of the disease; this delays an accurate diagnosis, causing patients to experience prolonged pain without proper treatment. We herein describe a 72-year-old woman and a 47-year-old woman with loin pain. They had undergone various imaging tests, but the cause of the pain remained unknown. Their pain was reproduced by the hooking maneuver, and twelfth rib syndrome was diagnosed. Both patients were immediately relieved of pain after a twelfth intercostal nerve block. Early diagnosis and appropriate treatment are needed for pain relief in patients with twelfth rib syndrome.", "gemini": "Hội chứng xương sườn thứ mười hai là một tình trạng hiếm gặp gây đau dữ dội ở thắt lưng. Việc chẩn đoán hiện tượng này dựa trên tiền sử bệnh và các phát hiện khi khám sức khỏe của bệnh nhân. Tuy nhiên, nhiều bác sĩ lâm sàng vẫn chưa hiểu rõ về căn bệnh này; điều này làm chậm trễ chẩn đoán chính xác, khiến bệnh nhân phải chịu đau kéo dài mà không được điều trị thích hợp. Trong bài viết này, chúng tôi mô tả một phụ nữ 72 tuổi và một phụ nữ 47 tuổi bị đau thắt lưng. Họ đã trải qua nhiều xét nghiệm hình ảnh khác nhau, nhưng nguyên nhân gây đau vẫn chưa được biết. Cơn đau của họ tái phát khi thực hiện động tác móc và được chẩn đoán là hội chứng xương sườn thứ mười hai. Cả hai bệnh nhân đều được giảm đau ngay lập tức sau khi phong bế dây thần kinh liên sườn thứ mười hai. Cần chẩn đoán sớm và điều trị thích hợp để giảm đau cho những bệnh nhân mắc hội chứng xương sườn thứ mười hai."} +{"idx": 1880047, "en": "Visual loss after spine surgery in the prone position is a disastrous postoperative complication because it is almost irreversible. Additionally, the optimal treatments and recommended professional guidelines for visual loss after spine surgery are deficient. A 43-year-old man developed visual loss after spine surgery in the prone position. Immediate ophthalmic consultation confirmed central retinal artery occlusion. Therefore, combined therapies were administered, including neurotrophy, anticoagulation, vasodilation, and adequate fluid infusion, followed by hyperbaric oxygen treatment. After active treatment, his visual acuity gradually recovered from 5 hours postoperatively and continued to improve thereafter. We reviewed the literature on postoperative visual loss with a focus on spine surgery in the prone position. Because the etiology of this complication is complex and has few effective treatments, the best method for its avoidance is to pay close attention to preventing it during surgery.", "gemini": "Mất thị lực sau phẫu thuật cột sống ở tư thế nằm sấp là một biến chứng hậu phẫu thảm khốc vì nó hầu như không thể đảo ngược. Ngoài ra, các phương pháp điều trị tối ưu và hướng dẫn chuyên môn được khuyến nghị cho tình trạng mất thị lực sau phẫu thuật cột sống còn thiếu sót. Một người đàn ông 43 tuổi bị mất thị lực sau phẫu thuật cột sống ở tư thế nằm sấp. Tham vấn nhãn khoa ngay lập tức xác nhận tắc động mạch võng mạc trung tâm. Do đó, các liệu pháp kết hợp đã được áp dụng, bao gồm thần kinh dinh dưỡng, chống đông, giãn mạch và truyền đủ dịch, sau đó là điều trị oxy tăng áp. Sau khi điều trị tích cực, thị lực của anh ấy dần hồi phục từ 5 giờ sau phẫu thuật và tiếp tục cải thiện sau đó. Chúng tôi đã xem xét các tài liệu về mất thị lực sau phẫu thuật với trọng tâm là phẫu thuật cột sống ở tư thế nằm sấp. Vì nguyên nhân gây ra biến chứng này rất phức tạp và có ít phương pháp điều trị hiệu quả, nên phương pháp tốt nhất để tránh biến chứng này là chú ý phòng ngừa trong khi phẫu thuật."} +{"idx": 1880048, "en": "Adverse Childhood Experiences (ACEs) are multiple sources of maltreatment and household dysfunction with tremendous impact on health. A trauma-informed (TI) approach is preferred when working with patients with ACEs. The Professional ACEs-Informed Training for Health© (PATH©) educational program and simulation experience using standardized patients (SP) was developed to help healthcare professionals address ACEs with adults. PATH© is a 3-4 hour curriculum comprised of lecture and discussion, video-based demonstration, simulation experience, and debriefing. It was first developed for primary care (PC) residents in family medicine and internal medicine, and subsequently modified for occupational therapy (OT) and physical therapy (PT) students. This study evaluates a preliminary dataset focusing on PATH© skills of PC residents and OT and PT students during simulation. Recordings of 53 learner-SP encounters from 15 OT and PT students and 38 PC residents were coded using standardized behavioral codes. A subset of ten recordings of PC residents who participated in simulations in the first and fourth year of the training program allowed for evaluation of training outcomes over time. Results showed that medical residents and OT and PT students demonstrated skills during SP encounters congruent with TI training on addressing ACEs with adults, particularly in explaining ACEs, demonstrating empathy, collaborative treatment planning, and stigma reduction. PC residents showed both positive and negative changes in PATH©-specific skills from year 1 to 4 of the training program. This study supports the PATH© model and simulation-based training in preparing clinicians to address ACEs with adults and provides insight into further curriculum improvement.", "gemini": "Những trải nghiệm bất lợi trong thời thơ ấu (ACE) là nhiều nguồn ngược đãi và rối loạn chức năng trong gia đình có tác động to lớn đến sức khỏe. Một cách tiếp cận có hiểu biết về chấn thương (TI) được ưu tiên khi làm việc với những bệnh nhân có ACE. Chương trình giáo dục Đào tạo có hiểu biết về ACE chuyên nghiệp cho sức khỏe © (PATH ©) và trải nghiệm mô phỏng sử dụng bệnh nhân chuẩn hóa (SP) đã được phát triển để giúp các chuyên gia chăm sóc sức khỏe giải quyết ACE ở người lớn. PATH © là chương trình giảng dạy kéo dài 3-4 giờ bao gồm bài giảng và thảo luận, trình diễn dựa trên video, trải nghiệm mô phỏng và giải trình. Ban đầu, chương trình được phát triển cho các bác sĩ nội trú chăm sóc chính (PC) trong chuyên khoa gia đình và nội khoa, sau đó được sửa đổi cho sinh viên vật lý trị liệu (PT) và trị liệu nghề nghiệp (OT). Nghiên cứu này đánh giá một tập dữ liệu sơ bộ tập trung vào các kỹ năng PATH © của các bác sĩ nội trú PC và sinh viên OT và PT trong quá trình mô phỏng. Bản ghi của 53 cuộc gặp gỡ giữa người học và SP từ 15 sinh viên OT và PT và 38 bác sĩ nội trú PC đã được mã hóa bằng các mã hành vi chuẩn hóa. Một tập hợp con gồm mười bản ghi âm của các bác sĩ nội trú PC đã tham gia các buổi mô phỏng trong năm đầu tiên và năm thứ tư của chương trình đào tạo cho phép đánh giá kết quả đào tạo theo thời gian. Kết quả cho thấy các bác sĩ nội trú và sinh viên OT và PT đã thể hiện các kỹ năng trong các cuộc gặp gỡ SP phù hợp với đào tạo TI về giải quyết ACE ở người lớn, đặc biệt là giải thích về ACE, thể hiện sự đồng cảm, lập kế hoạch điều trị hợp tác và giảm kỳ thị. Các bác sĩ nội trú PC đã thể hiện cả những thay đổi tích cực và tiêu cực trong các kỹ năng cụ thể của PATH © từ năm 1 đến năm 4 của chương trình đào tạo. Nghiên cứu này hỗ trợ mô hình PATH © và đào tạo dựa trên mô phỏng trong việc chuẩn bị cho các bác sĩ lâm sàng giải quyết ACE ở người lớn và cung cấp thông tin chi tiết để cải thiện chương trình giảng dạy hơn nữa."} +{"idx": 1880049, "en": "BACKGROUND The American Council of Graduate Medical Education (ACGME) guidelines for scholarly activities by family medicine residents require at least one activity per resident and encourage conference presentations. Meeting these guidelines has traditionally been challenging due to a multitude of factors from lack of time to limited administrative support. Studies have shown that resident participation in research was associated with higher levels of satisfaction with training. We aimed to develop and evaluate the effectiveness of a dedicated research curriculum in achieving ACGME goals for our residents. METHODS We performed a need assessment that identified strengths and obstacles related to research which then guided the actions taken to build the curriculum. Revised curricular elements included a research focused lecture series, a restructured journal club, financial support for presentations and project expenses, a specific timeline for project completion, and the development of a regional research day involving multiple family medicine programs. Dedicated research time was built into the resident schedule and presentations at local, regional and national conferences were encouraged and supported. RESULTS Following implementation of the curriculum there was a marked increase in the number of scholarly projects performed by residents. Prior to implementation there had only been one presentation at a national conference in the previous five years. This increased to an average of four presentations per year in the following five year period. On a regional scale, the initial success of the local research day led to a continued expansion and now includes six family medicine programs. CONCLUSION Implementation of a dedicated multifaceted research curriculum significantly increased the participation of our residents in scholarly activities and led to a near five-fold increase in presentations at regional and national levels. Additionally, resident satisfaction in scholarly activities increased and a far greater number of graduating residents went on to complete fellowships.", "gemini": "HOÀN CẢNH Các hướng dẫn của Hội đồng Giáo dục Y khoa Sau đại học Hoa Kỳ (ACGME) về các hoạt động học thuật của các bác sĩ nội trú y học gia đình yêu cầu mỗi bác sĩ nội trú thực hiện ít nhất một hoạt động và khuyến khích các bài thuyết trình tại hội nghị. Theo truyền thống, việc đáp ứng các hướng dẫn này rất khó khăn do nhiều yếu tố từ thiếu thời gian đến hỗ trợ hành chính hạn chế. Các nghiên cứu đã chỉ ra rằng sự tham gia của các bác sĩ nội trú vào nghiên cứu có liên quan đến mức độ hài lòng cao hơn với đào tạo. Chúng tôi đặt mục tiêu phát triển và đánh giá hiệu quả của một chương trình giảng dạy nghiên cứu chuyên sâu trong việc đạt được các mục tiêu của ACGME đối với các bác sĩ nội trú của chúng tôi. PHƯƠNG PHÁP Chúng tôi đã thực hiện đánh giá nhu cầu để xác định các điểm mạnh và điểm yếu liên quan đến nghiên cứu, sau đó hướng dẫn các hành động được thực hiện để xây dựng chương trình giảng dạy. Các yếu tố chương trình giảng dạy được sửa đổi bao gồm một loạt bài giảng tập trung vào nghiên cứu, một câu lạc bộ tạp chí được tái cấu trúc, hỗ trợ tài chính cho các bài thuyết trình và chi phí dự án, một mốc thời gian cụ thể để hoàn thành dự án và phát triển một ngày nghiên cứu khu vực liên quan đến nhiều chương trình y học gia đình. Thời gian nghiên cứu chuyên sâu đã được đưa vào lịch trình của bác sĩ nội trú và các bài thuyết trình tại các hội nghị địa phương, khu vực và quốc gia được khuyến khích và hỗ trợ. KẾT QUẢ Sau khi triển khai chương trình giảng dạy, số lượng các dự án học thuật do các bác sĩ nội trú thực hiện đã tăng đáng kể. Trước khi triển khai, chỉ có một bài thuyết trình tại một hội nghị quốc gia trong năm năm trước. Con số này đã tăng lên trung bình bốn bài thuyết trình mỗi năm trong năm năm tiếp theo. Ở quy mô khu vực, thành công ban đầu của ngày nghiên cứu tại địa phương đã dẫn đến sự mở rộng liên tục và hiện bao gồm sáu chương trình y học gia đình. KẾT LUẬN Việc triển khai một chương trình giảng dạy nghiên cứu đa dạng chuyên sâu đã làm tăng đáng kể sự tham gia của các bác sĩ nội trú của chúng tôi vào các hoạt động học thuật và dẫn đến sự gia tăng gần gấp năm lần các bài thuyết trình ở cấp khu vực và quốc gia. Ngoài ra, sự hài lòng của các bác sĩ nội trú trong các hoạt động học thuật đã tăng lên và số lượng bác sĩ nội trú tốt nghiệp tiếp tục hoàn thành các học bổng cao hơn nhiều."} +{"idx": 1880050, "en": "The profession of occupational therapy was formalized in the USA in 1917. Many of its earliest proponents were psychiatrists, yet their role in the development of the profession has received limited attention. This paper addresses this gap by considering one of the earliest Irish psychiatrist patrons of occupational therapy: Dr Eamon O'Sullivan (1897-1966) of Killarney Mental Hospital, Co Kerry, who developed an occupational therapy department in 1934. A textbook written by O'Sullivan reflects core philosophies articulated by occupational therapy's founders, and these philosophies were evident in practice at his hospital. Some inconsistencies between O'Sullivan's writings and practice are identified. In the absence of patient testimonies, it is not possible to resolve questions about the potential exploitation of patients through work as therapy.", "gemini": "Nghề trị liệu nghề nghiệp được chính thức hóa tại Hoa Kỳ vào năm 1917. Nhiều người ủng hộ ban đầu của nghề này là bác sĩ tâm thần, nhưng vai trò của họ trong sự phát triển của nghề này lại ít được chú ý. Bài báo này giải quyết khoảng cách này bằng cách xem xét một trong những người bảo trợ tâm thần sớm nhất của Ireland về liệu pháp nghề nghiệp: Tiến sĩ Eamon O'Sullivan (1897-1966) của Bệnh viện Tâm thần Killarney, Quận Kerry, người đã phát triển một khoa trị liệu nghề nghiệp vào năm 1934. Một cuốn sách giáo khoa do O'Sullivan viết phản ánh các triết lý cốt lõi được các nhà sáng lập ra liệu pháp nghề nghiệp nêu ra và những triết lý này đã được thể hiện rõ ràng trong quá trình thực hành tại bệnh viện của ông. Một số điểm không nhất quán giữa các bài viết và thực hành của O'Sullivan đã được xác định. Khi không có lời khai của bệnh nhân, không thể giải quyết các câu hỏi về khả năng bóc lột bệnh nhân thông qua công việc như liệu pháp."} +{"idx": 1880051, "en": "On April 28, 2019, Dr. Beat Steiner, president of the Society of Teachers of Family Medicine (STFM), proposed that we as family medicine educators focus on increasing kindness and compassion in our lives and the world around us. As teachers of Family Medicine, we are poised in the intersections among healing, teaching, training, and caregiving. Faculty are held accountable for ensuring that our learners achieve certain milestones by demonstrating defined clinical competencies. We have a long list of topics, biomedical and psychosocial, to be sure our learners understand, and procedures of all types that our learners must do. This paper discusses the importance of extending kindness to ourselves, our colleagues, and patients, and explores three \"pro-social\" emotions-compassion, gratitude, and awe, which health professions educators can model for trainees in the service of developing self-aware, relationally competent, and well-rounded clinicians.", "gemini": "Vào ngày 28 tháng 4 năm 2019, Tiến sĩ Beat Steiner, chủ tịch Hiệp hội các Giáo viên Y học Gia đình (STFM), đã đề xuất rằng chúng ta với tư cách là những nhà giáo dục y học gia đình tập trung vào việc gia tăng lòng tốt và lòng trắc ẩn trong cuộc sống của chúng ta và thế giới xung quanh chúng ta. Là những người giảng dạy Y học gia đình, chúng ta đang đứng ở vị trí giao thoa giữa chữa bệnh, giảng dạy, đào tạo và chăm sóc. Khoa được coi là chịu trách nhiệm đảm bảo rằng những người học của chúng ta đạt được những cột mốc nhất định bằng cách chứng minh các năng lực lâm sàng được xác định. Chúng ta có một danh sách dài các chủ đề, y sinh học và tâm lý xã hội, để đảm bảo rằng những người học của chúng ta hiểu, và các thủ thuật thuộc mọi loại mà những người học của chúng ta phải thực hiện. Bài báo này thảo luận về tầm quan trọng của việc mở rộng lòng tốt đối với bản thân, đồng nghiệp và bệnh nhân của chúng ta và khám phá ba cảm xúc \"thân xã hội\" - lòng trắc ẩn, lòng biết ơn và sự kính sợ, mà những nhà giáo dục về ngành y tế có thể làm gương cho những người học việc để phục vụ cho mục đích phát triển các bác sĩ lâm sàng có nhận thức về bản thân, có năng lực quan hệ và toàn diện."} +{"idx": 1880052, "en": "Bipolar disorder is a psychiatric illness that is relatively common among patients presenting for treatment in primary care clinics. Physicians in primary care often face difficult decisions about how and when to intervene when a patient is experiencing depressive, manic, or hypomanic episodes consistent with bipolar disorder. This article reviews the literature on how to assess and diagnose bipolar disorder in primary care, and how to choose from the array of treatment options that exist. The psychotherapy and pharmacotherapy evidence base provides guidance on how to help patients effectively manage this ailment. Collaboration among health and mental health practitioners is key in helping manage the \"peaks and valleys\" of bipolar disorder. Special considerations need to be made to routinely assess for impulsivity, suicidality, and patient progress throughout the course of treatment.", "gemini": "Rối loạn lưỡng cực là một bệnh tâm thần tương đối phổ biến ở những bệnh nhân đến khám tại các phòng khám chăm sóc ban đầu. Các bác sĩ chăm sóc ban đầu thường phải đối mặt với những quyết định khó khăn về cách thức và thời điểm can thiệp khi bệnh nhân đang trải qua các cơn trầm cảm, hưng cảm hoặc hưng nhẹ phù hợp với rối loạn lưỡng cực. Bài viết này xem xét các tài liệu về cách đánh giá và chẩn đoán rối loạn lưỡng cực trong chăm sóc ban đầu và cách lựa chọn từ nhiều lựa chọn điều trị hiện có. Bằng chứng về liệu pháp tâm lý và dược lý trị liệu cung cấp hướng dẫn về cách giúp bệnh nhân quản lý căn bệnh này một cách hiệu quả. Sự hợp tác giữa các học viên sức khỏe và sức khỏe tâm thần là chìa khóa trong việc giúp quản lý các \"đỉnh và thung lũng\" của rối loạn lưỡng cực. Cần cân nhắc đặc biệt để đánh giá thường xuyên về tính bốc đồng, ý định tự tử và tiến triển của bệnh nhân trong suốt quá trình điều trị."} +{"idx": 1880053, "en": "In order to investigate the patient experience of integrated behavioral health care in primary care settings, we implemented a patient cohort model from a combined site sample (N = 727) consisting of a family practice clinic and a Federally Qualified Health Center. Patient experience was measured using 12 questions from a validated measure, the Agency for Healthcare Research and Quality's Consumer Assessment of Health Care Providers and Systems (CAHPS®), Home and Community Based Services version, and six additional questions about interactions with an integrated behavioral health care team. We assessed bivariate relationships between satisfaction with integration and the clinic practice and self-reported physical health or self-reported mental/emotional health. We also utilized multiple regression to evaluate this relationship. Our analyses showed a statistically significant and small to moderate direct correlation between patients' self-reported health (both physical and mental/emotional health) and their ratings of the practice as a whole (p = .0003), such that patients who rated their physical and/or mental/emotional health as better were more likely to rate their overall satisfaction with the practice higher. The results of this study suggest that primary care patients with only mild to moderate health conditions (physical and/or mental/emotional) may experience greater satisfaction with integrated behavioral health care than patients with multiple and/or severe health conditions. In contrast, patients with multiple and/or severe health conditions may experience lower satisfaction with integrated behavioral health care and may be better served through higher levels of care.", "gemini": "Để nghiên cứu trải nghiệm của bệnh nhân về dịch vụ chăm sóc sức khỏe hành vi tích hợp trong các cơ sở chăm sóc ban đầu, chúng tôi đã triển khai một mô hình nhóm bệnh nhân từ một mẫu địa điểm kết hợp (N = 727) bao gồm một phòng khám gia đình và một Trung tâm Y tế đủ điều kiện của Liên bang. Trải nghiệm của bệnh nhân được đo lường bằng 12 câu hỏi từ một biện pháp được xác nhận, Đánh giá của Cơ quan Nghiên cứu và Chất lượng Chăm sóc Sức khỏe về Nhà cung cấp và Hệ thống Chăm sóc Sức khỏe (CAHPS®), phiên bản Dịch vụ Cộng đồng và Gia đình, và sáu câu hỏi bổ sung về tương tác với nhóm chăm sóc sức khỏe hành vi tích hợp. Chúng tôi đã đánh giá mối quan hệ giữa sự hài lòng với sự tích hợp và hoạt động thực hành của phòng khám và sức khỏe thể chất tự báo cáo hoặc sức khỏe tinh thần/cảm xúc tự báo cáo. Chúng tôi cũng sử dụng hồi quy đa để đánh giá mối quan hệ này. Các phân tích của chúng tôi cho thấy có một mối tương quan trực tiếp nhỏ và vừa phải có ý nghĩa thống kê giữa sức khỏe tự báo cáo của bệnh nhân (cả sức khỏe thể chất và sức khỏe tinh thần/cảm xúc) và đánh giá của họ về hoạt động thực hành nói chung (p = .0003), sao cho những bệnh nhân đánh giá sức khỏe thể chất và/hoặc sức khỏe tinh thần/cảm xúc của họ tốt hơn có nhiều khả năng đánh giá mức độ hài lòng chung của họ với hoạt động thực hành cao hơn. Kết quả của nghiên cứu này cho thấy rằng những bệnh nhân chăm sóc ban đầu chỉ mắc các tình trạng sức khỏe nhẹ đến trung bình (thể chất và/hoặc sức khỏe tinh thần/cảm xúc) có thể hài lòng hơn với dịch vụ chăm sóc sức khỏe hành vi tích hợp so với những bệnh nhân mắc nhiều tình trạng sức khỏe và/hoặc nghiêm trọng. Ngược lại, những bệnh nhân mắc nhiều tình trạng sức khỏe và/hoặc nghiêm trọng có thể ít hài lòng hơn với dịch vụ chăm sóc sức khỏe hành vi tích hợp và có thể được phục vụ tốt hơn thông qua các cấp độ chăm sóc cao hơn."} +{"idx": 1880054, "en": "Fifty years of scholarship by behavioral science faculty shaped the training and practice of family physicians. One can trace behavioral science influence from the philosophical underpinnings of family medicine (whole-person, patient-centered, biopsychosocial care), to the translation of psychotherapy skills into tools for family physicians, to the discovery of successful, integrated healthcare teams of family physicians and behavioral science professionals. Family medicine as we know it today rests on the shoulders of scholars who collaborated to conceive, experiment, evaluate, improve, and innovate. The purpose of this paper is to recruit you - behavioral science professionals - to continue this tradition of scholarship in Family Medicine. To advance your scholarly efforts, start by understanding that \"scholarship\" is not limited to research. \"Scholarship\" is sharing your good work with other professionals; through that interchange, you and they can improve. Next, surround yourself with people who will help you learn and succeed: mentors, collaborators, and helpers. Select a focus for your scholarship and read scientific literature for a thorough understanding of the topic. Identify a methodology that fits your values, interests, skills and resources. Start small, then grow. Sharpen your skills in writing and, if necessary, in statistics. Finally, Act Scholarly! by sharing your work with other behavioral science scholars.", "gemini": "Năm mươi năm học bổng của khoa học hành vi đã định hình nên quá trình đào tạo và thực hành của các bác sĩ gia đình. Người ta có thể thấy ảnh hưởng của khoa học hành vi từ nền tảng triết học của y học gia đình (chăm sóc toàn diện, lấy bệnh nhân làm trung tâm, chăm sóc sinh học tâm lý xã hội), đến việc chuyển đổi các kỹ năng trị liệu tâm lý thành công cụ cho các bác sĩ gia đình, đến khám phá các nhóm chăm sóc sức khỏe tích hợp thành công của các bác sĩ gia đình và các chuyên gia khoa học hành vi. Y học gia đình như chúng ta biết ngày nay dựa trên những học giả đã hợp tác để hình thành, thử nghiệm, đánh giá, cải tiến và đổi mới. Mục đích của bài báo này là tuyển dụng bạn - các chuyên gia khoa học hành vi - để tiếp tục truyền thống học bổng này trong Y học gia đình. Để thúc đẩy các nỗ lực học thuật của bạn, hãy bắt đầu bằng cách hiểu rằng \"học bổng\" không chỉ giới hạn ở nghiên cứu. \"Học bổng\" là chia sẻ công việc tốt của bạn với các chuyên gia khác; thông qua sự trao đổi đó, bạn và họ có thể cải thiện. Tiếp theo, hãy vây quanh mình với những người sẽ giúp bạn học hỏi và thành công: cố vấn, cộng tác viên và người giúp đỡ. Chọn trọng tâm cho học bổng của bạn và đọc tài liệu khoa học để hiểu sâu về chủ đề này. Xác định một phương pháp luận phù hợp với các giá trị, sở thích, kỹ năng và nguồn lực của bạn. Bắt đầu từ những điều nhỏ, sau đó phát triển. Rèn luyện các kỹ năng viết của bạn và nếu cần, hãy học thống kê. Cuối cùng, hãy hành động một cách học thuật! bằng cách chia sẻ công việc của bạn với các học giả khoa học hành vi khác."} +{"idx": 1880055, "en": "Health care workers engaged in work that they find personally meaningful are less likely to experience professional burnout. However, health care often involves tedious and burdensome tasks. While physicians are often asked to complete tasks that are less satisfying, creating space to focus on the meaningful parts of patient care is helpful to changing the focus of the mind. We report on the integration of Meaning in Medicine groups in a faculty development program at family medicine residency programs. These groups were created to increase faculty satisfaction, team cohesion, and engagement. Each session starts with an item for reflection - a video, article, or story - and is designed to provide a safe space for discussion of the joys in medicine. Group sizes of 8 to 15, dedicated time, and establishment of ground rules have been essential for success of these meetings.", "gemini": "Những người làm công tác chăm sóc sức khỏe tham gia vào công việc mà họ thấy có ý nghĩa cá nhân ít có khả năng bị kiệt sức nghề nghiệp. Tuy nhiên, chăm sóc sức khỏe thường liên quan đến các nhiệm vụ tẻ nhạt và nặng nề. Trong khi các bác sĩ thường được yêu cầu hoàn thành các nhiệm vụ ít thỏa mãn hơn, thì việc tạo ra không gian để tập trung vào các phần có ý nghĩa trong việc chăm sóc bệnh nhân sẽ hữu ích để thay đổi trọng tâm của tâm trí. Chúng tôi báo cáo về việc tích hợp các nhóm Ý nghĩa trong Y học vào chương trình phát triển khoa tại các chương trình nội trú y học gia đình. Các nhóm này được thành lập để tăng sự hài lòng của giảng viên, sự gắn kết của nhóm và sự tham gia. Mỗi buổi họp bắt đầu bằng một mục để suy ngẫm - một video, bài báo hoặc câu chuyện - và được thiết kế để tạo ra một không gian an toàn để thảo luận về những niềm vui trong y học. Quy mô nhóm từ 8 đến 15 người, thời gian dành riêng và thiết lập các quy tắc cơ bản là điều cần thiết cho sự thành công của các cuộc họp này."} +{"idx": 1880056, "en": "Physicians and other allied health professionals have many distractions from their work and from original motivations to become health caring professionals. Activities that detract from making meaningful connection with patients result in high levels of work dissatisfaction and burnout even at early stages of career or training. Narrative Medicine provides an antidote to these influences. It is an experiential discipline that draws on the Arts and Humanities, connects health professionals to their original motivation to care, cultivates the ability to engage patients and stimulates professional growth. When practiced with interdisciplinary teams, commonalities and mutual purpose are highlighted, promoting group cohesion and appreciation. The practice of this discipline and development of narrative competence relates closely to the advancement along numerous milestones, particularly Patient Care, Interpersonal and Communication Skills, and Professionalism. This article describes an experiential and didactic workshop presented at the 2019 Forum for Behavioral Science in Family Medicine which outlined a Narrative Medicine curriculum as taught at a community hospital Family Medicine residency. The curriculum is aimed at promoting residents' professional development, personal wellbeing, and capacity to engage patients.", "gemini": "Các bác sĩ và các chuyên gia y tế liên quan khác có nhiều sự xao nhãng khỏi công việc của họ và từ động lực ban đầu để trở thành những chuyên gia chăm sóc sức khỏe. Các hoạt động làm giảm sự kết nối có ý nghĩa với bệnh nhân dẫn đến mức độ không hài lòng với công việc và kiệt sức ngay cả ở giai đoạn đầu của sự nghiệp hoặc đào tạo. Y học tự sự cung cấp một phương thuốc giải độc cho những ảnh hưởng này. Đây là một ngành học dựa trên kinh nghiệm, lấy cảm hứng từ Nghệ thuật và Nhân văn, kết nối các chuyên gia y tế với động lực chăm sóc ban đầu của họ, bồi dưỡng khả năng thu hút bệnh nhân và kích thích sự phát triển chuyên môn. Khi thực hành với các nhóm liên ngành, các điểm chung và mục đích chung được nêu bật, thúc đẩy sự gắn kết và đánh giá cao của nhóm. Việc thực hành chuyên môn này và phát triển năng lực tự sự có liên quan chặt chẽ đến sự tiến bộ theo nhiều cột mốc, đặc biệt là Chăm sóc bệnh nhân, Kỹ năng giao tiếp và quan hệ giữa các cá nhân và Chuyên môn. Bài viết này mô tả một hội thảo trải nghiệm và giáo khoa được trình bày tại Diễn đàn Khoa học Hành vi năm 2019 trong Y học Gia đình, trong đó phác thảo chương trình giảng dạy Y học Tự sự như được giảng dạy tại khoa Y học Gia đình của một bệnh viện cộng đồng. Chương trình giảng dạy nhằm mục đích thúc đẩy sự phát triển chuyên môn của học viên, sức khỏe cá nhân và khả năng thu hút bệnh nhân."} +{"idx": 1880057, "en": "Technology in medicine has been rapidly evolving over the past decade, greatly improving the quality and types of services providers can offer to patients. Physicians in training are eager to embrace these novel innovations, and medical school and residency educators strive to offer learning experiences of a high standard that are relevant. One example of an emerging healthcare innovation is telemedicine, which permits the provision of medical care to patients away from clinics and hospitals, bringing patient-centered care to the patient. It has proven to be cost-effective, improve health outcomes, and enhance patient satisfaction. This article describes the development and structure of our family medicine residency program's telemedicine curriculum, first created in 2016 in response to the growing demand for this type of healthcare delivery model. There is discussion about the history of telemedicine, and about what contributed to its growth. A timeline of the steps taken to create our new telemedicine residency curriculum is reviewed, along with the key components that contributed to its success. The Lessons Learned section provides other educators insight into the strengths and opportunities revealed during the creation of the curriculum, and guidance on how the curriculum could be further enhanced.", "gemini": "Công nghệ trong y học đã phát triển nhanh chóng trong thập kỷ qua, cải thiện đáng kể chất lượng và loại dịch vụ mà nhà cung cấp có thể cung cấp cho bệnh nhân. Các bác sĩ đang trong quá trình đào tạo rất muốn nắm bắt những đổi mới mới này và các nhà giáo dục trường y và nội trú cố gắng cung cấp các trải nghiệm học tập có chất lượng cao và có liên quan. Một ví dụ về đổi mới chăm sóc sức khỏe mới nổi là y học từ xa, cho phép cung cấp dịch vụ chăm sóc y tế cho bệnh nhân ở xa các phòng khám và bệnh viện, mang lại dịch vụ chăm sóc lấy bệnh nhân làm trung tâm đến với bệnh nhân. Nó đã được chứng minh là tiết kiệm chi phí, cải thiện kết quả sức khỏe và tăng sự hài lòng của bệnh nhân. Bài viết này mô tả sự phát triển và cấu trúc của chương trình đào tạo y khoa gia đình của chúng tôi về chương trình giảng dạy y học từ xa, lần đầu tiên được tạo ra vào năm 2016 để đáp ứng nhu cầu ngày càng tăng đối với mô hình cung cấp dịch vụ chăm sóc sức khỏe loại này. Có thảo luận về lịch sử của y học từ xa và về những gì đã góp phần vào sự phát triển của nó. Một dòng thời gian về các bước thực hiện để tạo ra chương trình đào tạo y khoa từ xa mới của chúng tôi được xem xét, cùng với các thành phần chính góp phần vào thành công của nó. Phần Bài học kinh nghiệm cung cấp cho các nhà giáo dục khác hiểu biết sâu sắc về những điểm mạnh và cơ hội được tiết lộ trong quá trình tạo ra chương trình giảng dạy và hướng dẫn về cách chương trình giảng dạy có thể được nâng cao hơn nữa."} +{"idx": 1880058, "en": "PURPOSE Striatal asymmetry is a common feature in Parkinson's disease (PD), which changes with the progression of the disease. However, the correlation between the striatal asymmetry and severity of PD remains unclear. The present study aimed to investigate the characteristics of asymmetry in PD, and analyze the correlation between the striatal asymmetry index (SAI) and disease severity. MATERIALS AND METHODS This retrospective study enrolled 63 patients with idiopathic PD. The severity of PD was classified according to the Hoehn & Yahr (H&Y) staging system. The SAI in the subregions of the striatum was measured using 11C-N-2-carbomethoxy-3-(4-fluorophenyl)-tropane (11C-CFT) positron emission tomography (PET). RESULTS There was a significant difference in the SAI of the posterior putamen among the three groups (H&Y stage I, H&Y stage II, and H&Y stage III-IV; p = 0.001). However, there was no difference in the SAI of the anterior putamen (p = 0.340) or SAI of the caudate nucleus (p = 0.342) among the three groups. The SAI of the posterior putamen in patients with PD was significantly higher than that in patients with multiple system atrophy or progressive supranuclear palsy (p = 0.008). CONCLUSION The SAI of the posterior putamen is associated with the severity of PD, and may be correlated to the loss of dopamine cells in the pars compacta of the ventrolateral substantia nigra projecting to the posterior putamen. The SAI may be a potential indicator for evaluating the severity of PD, and distinguishing PD from other degenerative diseases.", "gemini": "MỤC ĐÍCH Tính không đối xứng của thể vân là một đặc điểm phổ biến trong bệnh Parkinson (PD), thay đổi theo tiến trình của bệnh. Tuy nhiên, mối tương quan giữa tính không đối xứng của thể vân và mức độ nghiêm trọng của PD vẫn chưa rõ ràng. Nghiên cứu hiện tại nhằm mục đích tìm hiểu các đặc điểm của tính không đối xứng trong PD và phân tích mối tương quan giữa chỉ số không đối xứng của thể vân (SAI) và mức độ nghiêm trọng của bệnh. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu hồi cứu này đã đăng ký 63 bệnh nhân mắc PD vô căn. Mức độ nghiêm trọng của PD được phân loại theo hệ thống phân loại Hoehn & Yahr (H&Y). SAI ở các vùng phụ của thể vân được đo bằng chụp cắt lớp phát xạ positron (PET) 11C-N-2-carbomethoxy-3-(4-fluorophenyl)-tropane (11C-CFT). KẾT QUẢ Có sự khác biệt đáng kể về SAI của nhân đuôi sau giữa ba nhóm (giai đoạn H&Y I, giai đoạn H&Y II và giai đoạn H&Y III-IV; p = 0,001). Tuy nhiên, không có sự khác biệt về SAI của nhân đuôi trước (p = 0,340) hoặc SAI của nhân đuôi (p = 0,342) giữa ba nhóm. SAI của nhân đuôi sau ở những bệnh nhân mắc PD cao hơn đáng kể so với những bệnh nhân mắc teo hệ thống đa hoặc liệt trên nhân vận động tiến triển (p = 0,008). KẾT LUẬN SAI của nhân đuôi sau có liên quan đến mức độ nghiêm trọng của PD và có thể tương quan với sự mất tế bào dopamin ở phần đặc của chất đen bụng bên chiếu đến nhân đuôi sau. SAI có thể là một chỉ số tiềm năng để đánh giá mức độ nghiêm trọng của PD và phân biệt PD với các bệnh thoái hóa khác."} +{"idx": 1880059, "en": "BACKGROUND Congenital heart defects(CHDs) are an important cause of neonatal mortality and morbidity. With advances in diagnosis and treatment, many defects are now amenable to correction. There is a need for individualized approach to prenatally detected lesions to predict the likely prognosis. Assigning them into risk category helps in prenatal counseling, decision making, referrals and formulation of management plan to improve the outcome. OBJECTIVE To grade the fetal CHDs according to severity and study its usefulness in decision making. METHODS A prospective study at a tertiary care institute between 2016 and 18, including pregnant women with antenatal diagnosis of fetal CHD. Detailed fetal echocardiography was followed by classification of lesions into four risk categories using modified grading system: (A) extremely high risk; (B) high risk (C) moderate risk (D) low risk. Appropriate counseling was provided to facilitate decision making and further management. The grading was reviewed and revised again postpartum/post-mortem for correlation. RESULTS Of the total 137 cases, almost half (45.53%) were Category B, while Category D, C and A had 24.1%, 20.4% and 10.2% of cases respectively. The mean gestation age at diagnosis was 26.5 weeks. Termination of pregnancy was done in 21 cases, mostly in Category B (71.4%) and of the 116 continued pregnancies, there were 16 intrauterine deaths. Prenatal and postnatal findings were available in 109 cases and kappa analysis for agreement between antenatal and postnatal grading showed good agreement (0.82). CONCLUSION Prenatal grading of congenital heart disease is a reliable, structured and simplified tool that can be used for providing counseling and facilitate decision making.", "gemini": "BỐI CẢNH Các khuyết tật tim bẩm sinh (CHD) là một nguyên nhân quan trọng gây tử vong và bệnh tật ở trẻ sơ sinh. Với những tiến bộ trong chẩn đoán và điều trị, nhiều khuyết tật hiện đã có thể khắc phục. Cần có phương pháp tiếp cận cá nhân hóa đối với các tổn thương được phát hiện trước khi sinh để dự đoán tiên lượng có thể xảy ra. Việc phân loại chúng vào nhóm rủi ro giúp ích cho việc tư vấn trước sinh, ra quyết định, giới thiệu và xây dựng kế hoạch quản lý để cải thiện kết quả. MỤC TIÊU Đánh giá các CHD của thai nhi theo mức độ nghiêm trọng và nghiên cứu tính hữu ích của nó trong việc ra quyết định. PHƯƠNG PHÁP Một nghiên cứu triển vọng tại một học viện chăm sóc sức khỏe bậc ba từ năm 2016 đến 18, bao gồm những phụ nữ mang thai được chẩn đoán trước khi sinh về CHD của thai nhi. Siêu âm tim thai chi tiết sau đó là phân loại các tổn thương thành bốn nhóm rủi ro bằng cách sử dụng hệ thống phân loại đã sửa đổi: (A) rủi ro cực kỳ cao; (B) rủi ro cao (C) rủi ro trung bình (D) rủi ro thấp. Tư vấn thích hợp đã được cung cấp để tạo điều kiện cho việc ra quyết định và quản lý thêm. Phân loại đã được xem xét và sửa đổi lại sau sinh/sau tử để đối chiếu. KẾT QUẢ Trong tổng số 137 trường hợp, gần một nửa (45,53%) thuộc Loại B, trong khi Loại D, C và A có 24,1%, 20,4% và 10,2% trường hợp tương ứng. Tuổi thai trung bình khi chẩn đoán là 26,5 tuần. Phá thai được thực hiện ở 21 trường hợp, chủ yếu ở Loại B (71,4%) và trong số 116 trường hợp tiếp tục mang thai, có 16 trường hợp tử vong trong tử cung. Các phát hiện trước sinh và sau sinh đã có ở 109 trường hợp và phân tích kappa để thống nhất giữa phân loại trước sinh và sau sinh cho thấy sự thống nhất tốt (0,82). KẾT LUẬN Phân loại trước sinh đối với bệnh tim bẩm sinh là một công cụ đáng tin cậy, có cấu trúc và đơn giản hóa có thể được sử dụng để cung cấp tư vấn và tạo điều kiện cho việc ra quyết định."} +{"idx": 1880060, "en": "BACKGROUND Traumatic occipitocervical dislocation (OCD) occurs due to fatal high-energy injury. Modern screw-based constructs enable successful reduction and stabilisation. In view of this, there are no previous reports on the spontaneous remodelling of the O-C1 joint after posterior fusion. We report the first case of postoperative spontaneous remodelling and stabilisation of the O-C1 joint after traumatic OCD.Case description: A 9-year-old girl suffered from traumatic OCD, accompanied by complete rupture of the O-C1-C2 ligamentous complex. Halo-vest fixation, and subsequently posterior fusion surgery from the occipital bone to C2, with autologous iliac crest bone graft and an allograft were performed. However, we could not achieve complete reduction of the O-C1 joint during surgery owing to extremely severe instability.Postoperative X-ray and computed tomography scan showed incomplete reduction of the O-C1 joint. Insufficient congruity of the O-C1 joint persisted. Afterwards, gradual spontaneous remodelling of the O-C1 joint occurred, both anteriorly and posteriorly 3 months postoperatively. Solid union was achieved 6 months postoperatively. Two years later, bilateral O-C1 joints in the patient were completely reformed and restabilised by incredible vigorous remodelling. Insufficient reduction and persisting poor joint congruence after surgery for OCD was probably restabilised by further spontaneous remodelling of articular morphology in such a young patient. CONCLUSIONS Postoperative spontaneous remodelling of the O-C1 joint after posterior reconstruction for OCD may occur in young patients. Incomplete reduction of the O-C1 joint during surgery may be acceptable due to the possibility of postoperative bone remodelling and restabilisation.", "gemini": "BỐI CẢNH Trật khớp chẩm-cổ (OCD) do chấn thương xảy ra do chấn thương năng lượng cao gây tử vong. Các cấu trúc dựa trên ốc vít hiện đại cho phép giảm và ổn định thành công. Theo quan điểm này, không có báo cáo nào trước đây về quá trình tái tạo tự phát của khớp O-C1 sau khi cố định sau. Chúng tôi báo cáo trường hợp đầu tiên về tái tạo và ổn định tự phát sau phẫu thuật của khớp O-C1 sau OCD chấn thương. Mô tả trường hợp: Một bé gái 9 tuổi bị OCD chấn thương, kèm theo đứt hoàn toàn phức hợp dây chằng O-C1-C2. Đã thực hiện cố định halo-vest, và sau đó là phẫu thuật cố định sau từ xương chẩm đến C2, với ghép xương tự thân từ mào chậu và ghép dị ghép. Tuy nhiên, chúng tôi không thể giảm hoàn toàn khớp O-C1 trong quá trình phẫu thuật do tình trạng mất ổn định cực kỳ nghiêm trọng. Chụp X-quang và chụp cắt lớp vi tính sau phẫu thuật cho thấy giảm không hoàn toàn khớp O-C1. Sự không phù hợp của khớp O-C1 vẫn tồn tại. Sau đó, quá trình tái tạo tự phát dần dần của khớp O-C1 xảy ra, cả ở phía trước và phía sau 3 tháng sau phẫu thuật. Sự kết hợp vững chắc đạt được 6 tháng sau phẫu thuật. Hai năm sau, các khớp O-C1 song phương ở bệnh nhân đã được tái tạo hoàn toàn và ổn định lại nhờ quá trình tái tạo mạnh mẽ đáng kinh ngạc. Giảm không đủ và sự phù hợp khớp kém dai dẳng sau phẫu thuật OCD có thể được ổn định lại bằng quá trình tái tạo tự phát hơn nữa của hình thái khớp ở bệnh nhân trẻ như vậy. KẾT LUẬN Quá trình tái tạo tự phát sau phẫu thuật của khớp O-C1 sau khi tái tạo sau cho OCD có thể xảy ra ở bệnh nhân trẻ. Giảm không hoàn toàn khớp O-C1 trong quá trình phẫu thuật có thể chấp nhận được do khả năng tái tạo xương và ổn định lại sau phẫu thuật."} +{"idx": 1880061, "en": "OBJECTIVE To assess the association between maternal height and birthweight in a healthy population and to study the effect of maternal height on the classification of birthweight as small for gestational age (SGA) and large for gestational age (LGA). METHODS A descriptive, observational retrospective study was conducted in a low risk population in the Netherlands. The study included term singleton healthy nonsmoking pregnant women with normal body mass index (n = 9291). We calculated the impact of maternal height on birthweight using multiple linear regression analyses with adjustment for gestational age, gender, and parity. We calculated the number of newborns classified as SGA and LGA using the cutoff point of the Dutch Birthweight chart, which does not customize for maternal height. Subsequently, we calculated the changes in classification from SGA and LGA to appropriate for gestational age (AGA) in case of customization for maternal height. RESULTS A significant association was found between maternal height and birthweight; 15.0 g higher birthweight per extra cm maternal height (95% confidence interval 13.8-16.1; p<.001; R2 model = 0.28). The incidence of SGA was 7.1% (range 17.4-2.0% form shortest to tallest maternal height category) and of LGA 8.4% (range 1.9-21.5% from shortest to tallest maternal height category). We calculated a shift in classification: 114 newborns (17.3%) in shorter (<167 cm) women previously SGA and 165 newborns (21.1%) in taller (>173 cm) women previously LGA were classified as AGA when controlling for maternal height. CONCLUSIONS Maternal height is significantly associated with birthweight. Birthweight charts customized for maternal height change classification in one out of six SGA or LGA newborns at term.", "gemini": "MỤC TIÊU Đánh giá mối liên quan giữa chiều cao của mẹ và cân nặng khi sinh ở một quần thể khỏe mạnh và nghiên cứu ảnh hưởng của chiều cao của mẹ đối với việc phân loại cân nặng khi sinh là nhỏ so với tuổi thai (SGA) và lớn so với tuổi thai (LGA). PHƯƠNG PHÁP Một nghiên cứu hồi cứu quan sát, mô tả đã được tiến hành trong một quần thể có nguy cơ thấp ở Hà Lan. Nghiên cứu bao gồm những phụ nữ mang thai khỏe mạnh, không hút thuốc, đủ tháng, có chỉ số khối cơ thể bình thường (n = 9291). Chúng tôi đã tính toán tác động của chiều cao của mẹ đối với cân nặng khi sinh bằng cách sử dụng phân tích hồi quy tuyến tính đa biến với điều chỉnh theo tuổi thai, giới tính và số lần sinh. Chúng tôi đã tính toán số trẻ sơ sinh được phân loại là SGA và LGA bằng cách sử dụng điểm cắt của biểu đồ cân nặng khi sinh của Hà Lan, biểu đồ này không tùy chỉnh theo chiều cao của mẹ. Sau đó, chúng tôi đã tính toán những thay đổi trong phân loại từ SGA và LGA sang phù hợp với tuổi thai (AGA) trong trường hợp tùy chỉnh theo chiều cao của mẹ. KẾT QUẢ Đã tìm thấy mối liên quan đáng kể giữa chiều cao của mẹ và cân nặng khi sinh; Cân nặng khi sinh cao hơn 15,0 g trên mỗi cm chiều cao của mẹ (khoảng tin cậy 95% 13,8-16,1; p < 0,001; mô hình R2 = 0,28). Tỷ lệ SGA là 7,1% (phạm vi 17,4-2,0% từ nhóm chiều cao của mẹ thấp nhất đến cao nhất) và LGA là 8,4% (phạm vi 1,9-21,5% từ nhóm chiều cao của mẹ thấp nhất đến cao nhất). Chúng tôi đã tính toán sự thay đổi trong phân loại: 114 trẻ sơ sinh (17,3%) ở những phụ nữ thấp hơn (<167 cm) trước đây là SGA và 165 trẻ sơ sinh (21,1%) ở những phụ nữ cao hơn (>173 cm) trước đây là LGA được phân loại là AGA khi kiểm soát chiều cao của mẹ. KẾT LUẬN Chiều cao của mẹ có liên quan đáng kể đến cân nặng khi sinh. Biểu đồ cân nặng khi sinh được tùy chỉnh theo chiều cao của mẹ làm thay đổi phân loại ở một trong sáu trẻ sơ sinh SGA hoặc LGA đủ tháng."} +{"idx": 1880062, "en": "PURPOSE An estimated 38 million and 50 million individuals will have cataract in the U.S. alone by 2030 and 2050, respectively. Breastfeeding is known to improve a number of health outcomes in both breastfed children and breastfeeding mothers. However, little is known about the relationship between breastfeeding and cataract, the leading cause of blindness worldwide, in breastfeeding mothers. This study was conducted to investigate the relationship between breastfeeding and maternal cataract extraction history in a nationally representative sample of the U.S. population. METHODS A 10-year multistage, probability-sampling survey data was used to identify parous women aged ≥50 years who provided breastfeeding history and cataract extraction history (n = 4897). Breastfeeding history was considered positive if a participant reported breastfeeding at least one child for ≥1 month. The main outcome was cataract extraction history. Estimates are presented in odds ratios (OR) and 95% confidence intervals (95%CI). RESULTS Approximately half of the studied women breastfed at least one child ≥1 month, and about 18% reported cataract extraction history. Participants with a positive breastfeeding history were less likely to have a positive cataract extraction history in both age-adjusted (OR = 0.814, 95%CI = 0.670-0.989) and multivariable logistic regression (OR = 0.794, 95%CI = 0.639-0.988). Higher number of breastfed children was also associated with a lower risk of cataract extraction history (OR = 0.934, 95%CI = 0.883-0.988). CONCLUSIONS The findings suggest that breastfeeding may be associated with a decrease in the likelihood of age-related cataract extraction in parous women from the U.S. population.", "gemini": "MỤC ĐÍCH Ước tính có 38 triệu và 50 triệu người sẽ bị đục thủy tinh thể chỉ riêng ở Hoa Kỳ vào năm 2030 và 2050. Cho con bú được biết là cải thiện một số kết quả sức khỏe ở cả trẻ bú mẹ và bà mẹ cho con bú. Tuy nhiên, người ta biết rất ít về mối quan hệ giữa việc cho con bú và bệnh đục thủy tinh thể, nguyên nhân hàng đầu gây mù trên toàn thế giới, ở những bà mẹ cho con bú. Nghiên cứu này được thực hiện để điều tra mối quan hệ giữa việc cho con bú và tiền sử phẫu thuật đục thủy tinh thể ở một mẫu đại diện toàn quốc của dân số Hoa Kỳ. PHƯƠNG PHÁP Dữ liệu khảo sát lấy mẫu xác suất nhiều giai đoạn trong 10 năm đã được sử dụng để xác định những phụ nữ đã sinh con từ 50 tuổi trở lên, những người cung cấp tiền sử cho con bú và tiền sử phẫu thuật đục thủy tinh thể (n = 4897). Tiền sử cho con bú được coi là tích cực nếu người tham gia báo cáo cho con bú ít nhất một đứa trẻ trong ≥1 tháng. Kết quả chính là tiền sử phẫu thuật đục thủy tinh thể. Ước tính được trình bày theo tỷ lệ tỷ lệ chênh lệch (OR) và khoảng tin cậy 95% (95% CI). KẾT QUẢ Khoảng một nửa số phụ nữ được nghiên cứu đã cho con bú ít nhất một đứa trẻ ≥1 tháng và khoảng 18% báo cáo tiền sử phẫu thuật đục thủy tinh thể. Những người tham gia có tiền sử cho con bú tích cực ít có khả năng có tiền sử phẫu thuật đục thủy tinh thể tích cực ở cả hai nhóm điều chỉnh theo tuổi (OR = 0,814, 95% CI = 0,670-0,989) và hồi quy logistic đa biến (OR = 0,794, 95% CI = 0,639-0,988). Số lượng trẻ được bú mẹ cao hơn cũng có liên quan đến nguy cơ tiền sử phẫu thuật đục thủy tinh thể thấp hơn (OR = 0,934, 95% CI = 0,883-0,988). KẾT LUẬN Những phát hiện cho thấy rằng việc cho con bú có thể liên quan đến việc giảm khả năng phẫu thuật đục thủy tinh thể liên quan đến tuổi ở những phụ nữ đã sinh con từ dân số Hoa Kỳ."} +{"idx": 1880063, "en": "OBJECTIVE To investigate effect of serum total testosterone and its relationship with other laboratory parameters on the prognosis of coronavirus disease 2019 (COVID-19) in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infected male patients. METHODS This prospective cohort study included 221 consecutive male patients (>18 years old) with laboratory confirmed SARS-CoV-2 who had been hospitalized due to COVID-19. The patients were divided into 3 groups: Asymptomatic patients (n: 46), symptomatic patients who were hospitalized in the internal medicine unit (IMU) (n: 129), and patients who were hospitalized in the intensive care unit (ICU) (n: 46). RESULTS As serum total testosterone level at baseline decreases, probability (%) to be in the ICU significantly increases (p = 0.001). As serum total testosterone level at baseline decreases, probability (%) of mortality significantly increases (p = 0.002). In the patients who had pre-COVID-19 serum gonadal hormones test (n: 24), serum total testosterone level significantly decreased from pre-COVID-19 level of 458 ± 198 ng/dl to 315 ± 120 ng/dl at the time of COVID-19 in the patients (p = 0.003). CONCLUSIONS COVID-19 might deteriorate serum testosterone level in SARS-CoV-2 infected male patients. Low serum total testosterone level at baseline has a significant increased risk for the ICU and mortality in patients with COVID-19.", "gemini": "MỤC TIÊU Nghiên cứu hiệu quả của tổng testosterone huyết thanh và mối quan hệ của nó với các thông số xét nghiệm khác đối với tiên lượng của bệnh do virus corona 2019 (COVID-19) ở những bệnh nhân nam bị nhiễm virus corona gây hội chứng hô hấp cấp tính nặng 2 (SARS-CoV-2). PHƯƠNG PHÁP Nghiên cứu theo dõi triển vọng này bao gồm 221 bệnh nhân nam liên tiếp (>18 tuổi) được xét nghiệm xác nhận mắc SARS-CoV-2 và đã được nhập viện do COVID-19. Những bệnh nhân này được chia thành 3 nhóm: Bệnh nhân không triệu chứng (n: 46), bệnh nhân có triệu chứng được nhập viện tại khoa nội khoa (IMU) (n: 129) và bệnh nhân được nhập viện tại khoa chăm sóc đặc biệt (ICU) (n: 46). KẾT QUẢ Khi nồng độ testosterone toàn phần trong huyết thanh lúc ban đầu giảm, khả năng (%) vào ICU tăng đáng kể (p = 0,001). Khi nồng độ testosterone toàn phần trong huyết thanh lúc ban đầu giảm, khả năng tử vong (%) tăng đáng kể (p = 0,002). Ở những bệnh nhân đã làm xét nghiệm nội tiết tố sinh dục trước khi mắc COVID-19 (n: 24), nồng độ testosterone toàn phần trong huyết thanh giảm đáng kể từ mức trước khi mắc COVID-19 là 458 ± 198 ng/dl xuống còn 315 ± 120 ng/dl tại thời điểm mắc COVID-19 ở những bệnh nhân (p = 0,003). KẾT LUẬN COVID-19 có thể làm giảm nồng độ testosterone trong huyết thanh ở những bệnh nhân nam bị nhiễm SARS-CoV-2. Nồng độ testosterone toàn phần trong huyết thanh thấp lúc ban đầu có nguy cơ vào ICU và tử vong tăng đáng kể ở những bệnh nhân mắc COVID-19."} +{"idx": 1880064, "en": "OBJECTIVES To assess understudied, alternative suicide prevention modalities in a mental health care setting. METHODS This was a prospective study of patients (n = 140, 68 cases and 72 controls) who were admitted to hospital or who contacted an SOS suicide crisis line for suicidal ideation or attempts. Psychiatric diagnoses (Mini-International Neuropsychiatric Interview) and intensity of anxiety/depression/suicidality (Hamilton Anxiety Rating Scale, Montgomery-Åsberg Depression Rating Scale, and Beck Scale for Suicidal Ideation) were assessed. All intervention group subjects received a crisis card with a crisis line number, interviews with psychologists or volunteers and a telephone call on days 10 to 21, then 6 months later. These subjects also had a choice between two further 4-month interventions: body contact care or mobile intervention team visits. RESULTS The interventions significantly reduced the number of suicide attempts and suicide (3%) at 6 months compared with the control condition (12%). There were fewer losses to follow-up in the intervention group (7.35%) than in the control group (9.72%). CONCLUSIONS The results favour the implementation of integrated care and maintaining contact in suicide prevention.", "gemini": "MỤC TIÊU Đánh giá các phương thức phòng ngừa tự tử thay thế chưa được nghiên cứu đầy đủ trong bối cảnh chăm sóc sức khỏe tâm thần. PHƯƠNG PHÁP Đây là một nghiên cứu triển vọng trên những bệnh nhân (n = 140, 68 ca và 72 đối chứng) được nhập viện hoặc liên hệ với đường dây nóng khủng hoảng tự tử SOS vì ý tưởng hoặc hành vi tự tử. Chẩn đoán tâm thần (Phỏng vấn tâm thần thần kinh quốc tế thu nhỏ) và mức độ lo lắng/trầm cảm/suy nghĩ tự tử (Thang đánh giá lo lắng Hamilton, Thang đánh giá trầm cảm Montgomery-Åsberg và Thang Beck về ý tưởng tự tử) đã được đánh giá. Tất cả các đối tượng trong nhóm can thiệp đều nhận được thẻ khủng hoảng có số đường dây nóng khủng hoảng, phỏng vấn với các nhà tâm lý học hoặc tình nguyện viên và cuộc gọi điện thoại vào ngày 10 đến 21, sau đó là 6 tháng sau. Những đối tượng này cũng có thể lựa chọn giữa hai biện pháp can thiệp thêm trong 4 tháng: chăm sóc tiếp xúc cơ thể hoặc các chuyến thăm của nhóm can thiệp di động. KẾT QUẢ Các biện pháp can thiệp đã làm giảm đáng kể số lần cố gắng tự tử và tự tử (3%) sau 6 tháng so với nhóm đối chứng (12%). Có ít trường hợp mất theo dõi hơn ở nhóm can thiệp (7,35%) so với nhóm đối chứng (9,72%). KẾT LUẬN Các kết quả ủng hộ việc thực hiện chăm sóc tích hợp và duy trì liên lạc trong công tác phòng ngừa tự tử."} +{"idx": 1880065, "en": "BACKGROUND Stent placement in the cannulation zone of AVF is becoming alarmingly frequent and sometimes in cases where surgical management would be more appropriate. When complications arise repair can be particularly challenging. We present our experience and management of this problem. METHODS We report our experience on three patients (two females and one male, mean age 72) that were referred to our department for problems related to stents placed at the cannulation segments. RESULTS All patients required complex surgical interventions. In two of them maintenance of access was possible but required the use of a graft. In one complete resection of the AVF and all stents was necessary, and the patient remained on dialysis through catheter. CONCLUSION Stent placement at the cannulation zones may complicate access management in the future and should be avoided if possible.", "gemini": "BỐI CẢNH Việc đặt stent ở vùng thông nối của AVF đang trở nên báo động thường xuyên và đôi khi trong những trường hợp mà việc quản lý phẫu thuật sẽ phù hợp hơn. Khi các biến chứng phát sinh, việc sửa chữa có thể đặc biệt khó khăn. Chúng tôi trình bày kinh nghiệm và cách quản lý vấn đề này của mình. PHƯƠNG PHÁP Chúng tôi báo cáo kinh nghiệm của mình về ba bệnh nhân (hai nữ và một nam, tuổi trung bình 72) được chuyển đến khoa của chúng tôi để giải quyết các vấn đề liên quan đến stent được đặt ở các đoạn thông nối. KẾT QUẢ Tất cả bệnh nhân đều cần can thiệp phẫu thuật phức tạp. Ở hai trong số họ, việc duy trì khả năng tiếp cận là khả thi nhưng cần phải sử dụng ghép. Trong một trường hợp phải cắt bỏ hoàn toàn AVF và tất cả các stent, và bệnh nhân vẫn phải chạy thận qua ống thông. KẾT LUẬN Việc đặt stent ở các vùng thông nối có thể làm phức tạp việc quản lý khả năng tiếp cận trong tương lai và nên tránh nếu có thể."} +{"idx": 1880066, "en": "INTRODUCTION A serious complication associated with Central Venous Access Device (CVAD) is infection because of bacterial contamination, either by the extra-luminal or by the intra-luminal route.We evaluated the efficacy, the safety, and the cost-effectiveness of two strategies for non-inferiority in controlling bacterial colonization of the exit-site of Peripherally-Inserted Central Catheters (PICC). METHODS After PICC placement, a skin swab of the exit site was taken and cultured. In group A the exit site was sealed with N-butyl-cyanoacrylate glue, while in group B a chlorhexidine-releasing sponge dressing was applied. A second skin culture was taken at day 7. RESULTS A total of 51 patients were enrolled in each group. In 42 patients the second skin culture was not performed because of 20 patients were lost at follow-up or deceased and in 22 patients the dressing needed to be changed early, because of local bleeding (13 cases, in group B) or because of dressing detachment (four in group A and five in group B). The microbiological study was completed in 36 patients in group A and 24 in group B. No microorganisms were isolated in any patient. CONCLUSIONS Both strategies were effective in controlling bacterial colonization. Glue was effective in reducing local bleeding, and it was more cost-effective than sponge dressing. During the first week, when local bleeding and bacterial colonization must be prevented, glue might be more appropriate than chlorhexidine-releasing dressing; after the first week chlorhexidine-releasing dressing might be preferable, considering that the safety of glue application on the skin for prolonged periods is still questionable.", "gemini": "GIỚI THIỆU Một biến chứng nghiêm trọng liên quan đến Thiết bị tiếp cận tĩnh mạch trung ương (CVAD) là nhiễm trùng do nhiễm khuẩn, có thể do đường ngoài lòng mạch hoặc đường trong lòng mạch. Chúng tôi đã đánh giá hiệu quả, độ an toàn và hiệu quả về chi phí của hai chiến lược không kém hơn trong việc kiểm soát tình trạng nhiễm khuẩn tại vị trí thoát của Ống thông trung tâm chèn ngoại vi (PICC). PHƯƠNG PHÁP Sau khi đặt PICC, lấy dịch tăm bông ở da tại vị trí thoát và nuôi cấy. Ở nhóm A, vị trí thoát được bịt kín bằng keo N-butyl-cyanoacrylate, trong khi ở nhóm B, sử dụng miếng băng xốp giải phóng chlorhexidine. Tiến hành nuôi cấy dịch tăm bông da thứ hai vào ngày thứ 7. KẾT QUẢ Tổng cộng có 51 bệnh nhân được ghi danh vào mỗi nhóm. Ở 42 bệnh nhân, không thực hiện nuôi cấy dịch tăm bông da thứ hai vì 20 bệnh nhân bị mất trong quá trình theo dõi hoặc đã tử vong và ở 22 bệnh nhân, cần thay băng sớm do chảy máu tại chỗ (13 trường hợp ở nhóm B) hoặc do băng bị bong (bốn trường hợp ở nhóm A và năm trường hợp ở nhóm B). Nghiên cứu vi sinh được hoàn thành ở 36 bệnh nhân ở nhóm A và 24 bệnh nhân ở nhóm B. Không có vi sinh vật nào được phân lập ở bất kỳ bệnh nhân nào. KẾT LUẬN Cả hai chiến lược đều có hiệu quả trong việc kiểm soát tình trạng nhiễm khuẩn. Keo có hiệu quả trong việc giảm chảy máu tại chỗ và hiệu quả về chi phí hơn so với băng xốp. Trong tuần đầu tiên, khi phải ngăn ngừa chảy máu tại chỗ và tình trạng nhiễm khuẩn, keo có thể phù hợp hơn so với băng giải phóng chlorhexidine; sau tuần đầu tiên, băng giải phóng chlorhexidine có thể được ưu tiên hơn, vì tính an toàn của việc sử dụng keo trên da trong thời gian dài vẫn còn là vấn đề đáng nghi ngờ."} +{"idx": 1880067, "en": "Objective: The goal of this project was to explore the initial psychometric properties (construct and ecological validity) of self-administered online (SAO) neuropsychological assessment (using the www.testmybrain.org platform), compared to traditional testing, in a clinical sample, as well as to evaluate participant acceptance. SAO assessment has the potential to expand the reach of in-person neuropsychological assessment approaches.Method: Counterbalanced, within-subjects design comparing SAO performance to in-person performance in adults with diabetes with and without Chronic Kidney Disease (CKD). Forty-nine participants completed both assessment modalities (type 1 diabetes N = 14, type 2 diabetes N = 35; CKD N = 18).Results: Associations between SAO and analogous in-person tests were adequate to good (r = 0.49-0.66). Association strength between divergent cognitive tests did not differ between SAO versus in-person tests. SAO testing was more strongly associated with age than in-person testing (age R2=0.54 versus 0.23), while prediction of education, HbA1c, and estimated glomerular filtration rate (eGFR) did not differ significantly between test modalities (education R2=0.37 versus 0.30; HbA1c R2=0.20 versus 0.12; eGFR R2 = 0.41 versus 0.33). Associations with measures of everyday functioning were also similar (Functional Activities Questionnaire R2=0.08 versus 0.07; Neuro-QoL R2=0.14 versus 0.16; Diabetes Self-Management Questionnaire R2=0.19 versus 0.19).Conclusions: The selected SAO neuropsychological tests had acceptable construct validity (including divergent, convergent, and criterion-related validity), and similar ecological validity to that of traditional testing. These SAO assessments were acceptable to participants and appear appropriate for use in research applications, although further research is needed to better understand the strengths and weaknesses in other clinical populations.", "gemini": "Mục tiêu: Mục tiêu của dự án này là khám phá các đặc tính trắc nghiệm ban đầu (giá trị xây dựng và sinh thái) của đánh giá thần kinh học trực tuyến tự quản (SAO) (sử dụng nền tảng www.testmybrain.org), so với xét nghiệm truyền thống, trong một mẫu lâm sàng, cũng như để đánh giá mức độ chấp nhận của người tham gia. Đánh giá SAO có tiềm năng mở rộng phạm vi tiếp cận của các phương pháp đánh giá thần kinh học trực tiếp. Phương pháp: Thiết kế đối chứng trong cùng đối tượng, so sánh hiệu suất SAO với hiệu suất trực tiếp ở những người lớn mắc bệnh tiểu đường có và không mắc Bệnh thận mãn tính (CKD). Bốn mươi chín người tham gia đã hoàn thành cả hai phương thức đánh giá (tiểu đường loại 1 N = 14, tiểu đường loại 2 N = 35; CKD N = 18). Kết quả: Các mối liên hệ giữa SAO và các xét nghiệm trực tiếp tương tự là từ vừa đủ đến tốt (r = 0,49-0,66). Mức độ liên kết giữa các xét nghiệm nhận thức khác nhau không khác nhau giữa xét nghiệm SAO so với xét nghiệm trực tiếp. Xét nghiệm SAO có liên quan mạnh hơn với tuổi so với xét nghiệm trực tiếp (tuổi R2 = 0,54 so với 0,23), trong khi khả năng dự đoán về trình độ học vấn, HbA1c và tốc độ lọc cầu thận ước tính (eGFR) không khác nhau đáng kể giữa các phương thức xét nghiệm (trình độ học vấn R2 = 0,37 so với 0,30; HbA1c R2 = 0,20 so với 0,12; eGFR R2 = 0,41 so với 0,33). Các mối liên hệ với các biện pháp hoạt động hàng ngày cũng tương tự nhau (Bản câu hỏi về hoạt động chức năng R2 = 0,08 so với 0,07; Neuro-QoL R2 = 0,14 so với 0,16; Bản câu hỏi tự quản lý bệnh tiểu đường R2 = 0,19 so với 0,19). Kết luận: Các xét nghiệm thần kinh học SAO được lựa chọn có giá trị xây dựng chấp nhận được (bao gồm giá trị phân kỳ, hội tụ và liên quan đến tiêu chuẩn) và giá trị sinh thái tương tự như xét nghiệm truyền thống. Những đánh giá SAO này được những người tham gia chấp nhận và dường như phù hợp để sử dụng trong các ứng dụng nghiên cứu, mặc dù cần nghiên cứu thêm để hiểu rõ hơn về điểm mạnh và điểm yếu trong các quần thể lâm sàng khác."} +{"idx": 1880068, "en": "Obstructive sleep apnoea (OSA) is a prevalent condition and has been extensively managed with orthognathic surgery using a variety of surgical techniques. This case report describes the successful management of a 56-year-old Caucasian woman with a bimaxillary retrusive profile and macroglossia complicated by OSA and the combined use of orthodontics and orthognathic surgery to improve Apnoea-Hypopnoea Index while maintaining facial aesthetics. The non-extraction treatment plan included: (1) pre-surgical orthodontic treatment to maximise aesthetics and functional occlusion after surgery; (2) maxillomandibular advancement using down fracture of the maxilla (Le Fort 1 osteotomy) with counter-clockwise rotation as well as bilateral sagittal split osteotomy with septoplasty to aid increase in airway function; and (3) post-surgical orthodontic finishing and alignment with self-ligating fixed appliances. Optimum aesthetic and functional results as well as an increase in the airway volume were achieved, without compromising facial aesthetics, with the cooperation of two specialties and the use of state-of-the-art technology during the surgical planning stages.", "gemini": "Ngưng thở khi ngủ do tắc nghẽn (OSA) là một tình trạng phổ biến và đã được điều trị rộng rãi bằng phẫu thuật chỉnh hình hàm mặt sử dụng nhiều kỹ thuật phẫu thuật khác nhau. Báo cáo trường hợp này mô tả sự điều trị thành công cho một phụ nữ da trắng 56 tuổi có cấu trúc hàm trên hàm dưới thụt lùi và tình trạng lưỡi to phức tạp do OSA và việc kết hợp sử dụng chỉnh nha và phẫu thuật chỉnh hình hàm để cải thiện Chỉ số ngưng thở-giảm thông khí trong khi vẫn duy trì tính thẩm mỹ của khuôn mặt. Kế hoạch điều trị không nhổ răng bao gồm: (1) điều trị chỉnh nha trước phẫu thuật để tối đa hóa tính thẩm mỹ và khớp cắn chức năng sau phẫu thuật; (2) phẫu thuật đẩy hàm trên hàm dưới bằng cách cắt xương hàm trên (phẫu thuật cắt xương Le Fort 1) với xoay ngược chiều kim đồng hồ cũng như phẫu thuật cắt xương tách đôi theo chiều đứng hai bên kết hợp với cắt vách ngăn mũi để hỗ trợ tăng chức năng đường thở; và (3) hoàn thiện chỉnh nha sau phẫu thuật và chỉnh thẳng răng bằng dụng cụ cố định tự buộc. Đã đạt được kết quả thẩm mỹ và chức năng tối ưu cũng như tăng thể tích đường thở, mà không ảnh hưởng đến tính thẩm mỹ của khuôn mặt, với sự hợp tác của hai chuyên khoa và sử dụng công nghệ tiên tiến trong các giai đoạn lập kế hoạch phẫu thuật."} +{"idx": 1880069, "en": "Climate change poses a grave threat to future generations, yet relatively little research examines children's understandings of the issue. This study examines the questions children ask about climate change - rather than their answers to adults' questions - exploring whether their questions suggest they view climate change as psychologically proximal or distant. Children aged 10-12 from 14 UK schools took part in an online event, asking scientists questions in a 'climate zone'. The questions were analysed using thematic analysis. The themes related to the nature and reality of climate change, its causes, impacts, and solutions. Participants seemed most exercised about the future impacts of and ways of ameliorating climate change, with some questions evoking science-fiction disaster imagery. The contents of participants' questions elucidated the ways in which they position climate change as both a proximal and distant phenomenon.", "gemini": "Biến đổi khí hậu gây ra mối đe dọa nghiêm trọng đối với các thế hệ tương lai, nhưng tương đối ít nghiên cứu xem xét sự hiểu biết của trẻ em về vấn đề này. Nghiên cứu này xem xét các câu hỏi mà trẻ em đặt ra về biến đổi khí hậu - thay vì câu trả lời của chúng cho các câu hỏi của người lớn - khám phá xem các câu hỏi của chúng có gợi ý rằng chúng coi biến đổi khí hậu là gần hay xa về mặt tâm lý hay không. Trẻ em trong độ tuổi từ 10-12 từ 14 trường học ở Vương quốc Anh đã tham gia một sự kiện trực tuyến, đặt câu hỏi cho các nhà khoa học trong một 'khu vực khí hậu'. Các câu hỏi đã được phân tích bằng cách phân tích chuyên đề. Các chủ đề liên quan đến bản chất và thực tế của biến đổi khí hậu, nguyên nhân, tác động và giải pháp của nó. Những người tham gia có vẻ quan tâm nhất đến những tác động trong tương lai và cách cải thiện biến đổi khí hậu, với một số câu hỏi gợi lên hình ảnh thảm họa khoa học viễn tưởng. Nội dung các câu hỏi của những người tham gia làm sáng tỏ cách họ định vị biến đổi khí hậu vừa là hiện tượng gần vừa là hiện tượng xa."} +{"idx": 1880070, "en": "Objective: The illness resulting from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), better known as COVID-19, has quickly escalated to a worldwide pandemic. Although understanding of the short and long-term manifestations of COVID-19 remains incomplete, there is a preponderance of respiratory pathology in COVID-19 and potential for chronic loss of pulmonary function in recovered patients, raising concerns for associated cognitive impacts.Method: We conducted a narrative review of the existing literature on neuropsychological variables in acute/severe respiratory disease and various forms of chronic pulmonary disease to inform expectations about potential cognitive manifestations of COVID-19.Results: Cognitive dysfunction is common but not inevitable in acute and chronic pulmonary disease, although unique predictors and symptom trajectories appear to be associated with each.Conclusions: Although the full scope of neuropathophysiology associated with COVID-19 remains to be established, pulmonary insults associated with the disease are likely to produce cognitive dysfunction in a substantial percentage of patients.", "gemini": "Mục tiêu: Bệnh do Hội chứng hô hấp cấp tính nặng do virus Corona 2 (SARS-CoV-2), hay còn gọi là COVID-19, đã nhanh chóng leo thang thành đại dịch trên toàn thế giới. Mặc dù sự hiểu biết về các biểu hiện ngắn hạn và dài hạn của COVID-19 vẫn còn chưa đầy đủ, nhưng có rất nhiều bệnh lý về hô hấp ở COVID-19 và khả năng mất chức năng phổi mãn tính ở những bệnh nhân đã hồi phục, làm dấy lên mối lo ngại về các tác động nhận thức liên quan. Phương pháp: Chúng tôi đã tiến hành đánh giá tường thuật về các tài liệu hiện có về các biến số tâm lý thần kinh trong bệnh hô hấp cấp tính / nặng và các dạng bệnh phổi mãn tính khác nhau để thông báo kỳ vọng về các biểu hiện nhận thức tiềm ẩn của COVID-19. Kết quả: Rối loạn nhận thức là phổ biến nhưng không phải là không thể tránh khỏi ở bệnh phổi cấp tính và mãn tính, mặc dù các yếu tố dự báo và quỹ đạo triệu chứng riêng biệt dường như có liên quan đến từng bệnh. Kết luận: Mặc dù phạm vi đầy đủ của bệnh lý thần kinh liên quan đến COVID-19 vẫn chưa được thiết lập, nhưng các tổn thương phổi liên quan đến bệnh này có khả năng gây ra rối loạn nhận thức ở một tỷ lệ đáng kể bệnh nhân."} +{"idx": 1880071, "en": "Obstetric antiphospholipid syndrome (APS) remains a clinical challenge for practitioners, with several controversial points that have not been answered so far. This Obstetric APS Task Force met on the 16th International Congress on Antiphospholipid Antibodies in Manchester, England, to discuss about treatment, diagnostic and clinical aspects of the disease. This report will address evidence-based medicine related to obstetric APS, including limitations on our current management, the relationship between antibodies against domain 1 of β2GPI and obstetric morbidity, hydroxychloroquine use in patients with obstetric APS and factors associated with thrombosis after obstetric APS. Finally, future directions for better understanding this complex condition are also reported by the Task Force coordinators.", "gemini": "Hội chứng kháng phospholipid sản khoa (APS) vẫn là một thách thức lâm sàng đối với các bác sĩ lâm sàng, với một số điểm gây tranh cãi vẫn chưa được giải đáp cho đến nay. Nhóm công tác APS sản khoa này đã họp tại Đại hội quốc tế lần thứ 16 về kháng thể kháng phospholipid tại Manchester, Anh, để thảo luận về các khía cạnh điều trị, chẩn đoán và lâm sàng của bệnh. Báo cáo này sẽ giải quyết các vấn đề về y học dựa trên bằng chứng liên quan đến APS sản khoa, bao gồm những hạn chế trong việc quản lý hiện tại của chúng tôi, mối quan hệ giữa kháng thể chống lại miền 1 của β2GPI và bệnh tật sản khoa, sử dụng hydroxychloroquine ở những bệnh nhân mắc APS sản khoa và các yếu tố liên quan đến huyết khối sau APS sản khoa. Cuối cùng, các hướng đi trong tương lai để hiểu rõ hơn về tình trạng phức tạp này cũng được các điều phối viên của Nhóm công tác báo cáo."} +{"idx": 1880072, "en": "OBJECTIVES The purpose of this study was to characterize the impact of cutaneous lupus erythematosus (CLE) in adults and identify the clinical and non-clinical factors associated with quality of life (QoL), using the Revised Wilson and Cleary Model. METHODS 101 patients diagnosed with CLE were included in this cross-sectional study. QoL was measured with the Cutaneous Lupus Erythematosus Quality of Life (CLEQoL) scale and disease activity and damage with the Cutaneous Lupus Activity and Severity Index (CLASI). Patient demographics, clinical, and disease characteristics were also collected. Descriptive statistics were calculated, and multiple regression was employed to determine significant (p < 0.05) predictors of overall QoL. Data were analyzed using SPSS v24. RESULTS The overall regression QoL model was significantly different from zero, (F = 24.96; df = 14, 76; p = <0.001). Disease activity (β = 0.13), pain (β = 0.13), fatigue (β = 0.24), body image (β = 0.62), and side effects (β = -0.13) were significant predictors of overall QoL while controlling for other predictor variables. Patients who experienced higher levels of disease activity, fatigue severity, pain levels, and greater degree of body dissatisfaction had significantly poorer QoL. Fewer side effects experienced from CLE medications were significantly associated with higher QoL. CONCLUSIONS Study findings support the considerable burden associated with CLE. Several modifiable variables such as pain, fatigue, body image, and disease activity were associated with QoL. Therefore, interventions that incorporate these variables may reduce negative impacts on QoL life and improve health outcomes in CLE patients. Furthermore, given the chronic and recurring nature of the condition, strategies focused on improving QoL are needed for this vulnerable population.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu này là mô tả tác động của bệnh lupus ban đỏ da (CLE) ở người lớn và xác định các yếu tố lâm sàng và phi lâm sàng liên quan đến chất lượng cuộc sống (QoL), sử dụng Mô hình Wilson và Cleary đã được sửa đổi. PHƯƠNG PHÁP 101 bệnh nhân được chẩn đoán mắc CLE đã được đưa vào nghiên cứu cắt ngang này. QoL được đo bằng thang đánh giá Chất lượng cuộc sống của bệnh lupus ban đỏ da (CLEQoL) và hoạt động và tổn thương của bệnh bằng Chỉ số hoạt động và mức độ nghiêm trọng của bệnh lupus ban đỏ da (CLASI). Dữ liệu nhân khẩu, lâm sàng và đặc điểm bệnh của bệnh nhân cũng được thu thập. Thống kê mô tả đã được tính toán và hồi quy đa biến được sử dụng để xác định các yếu tố dự báo quan trọng (p < 0,05) của QoL tổng thể. Dữ liệu được phân tích bằng SPSS v24. KẾT QUẢ Mô hình hồi quy QoL tổng thể khác biệt đáng kể so với 0, (F = 24,96; df = 14, 76; p = <0,001). Hoạt động của bệnh (β = 0,13), đau (β = 0,13), mệt mỏi (β = 0,24), hình ảnh cơ thể (β = 0,62) và tác dụng phụ (β = -0,13) là những yếu tố dự báo quan trọng của QoL tổng thể trong khi kiểm soát các biến dự báo khác. Những bệnh nhân có mức độ hoạt động của bệnh cao hơn, mức độ mệt mỏi nghiêm trọng, mức độ đau và mức độ không hài lòng về cơ thể cao hơn có QoL kém hơn đáng kể. Ít tác dụng phụ gặp phải từ thuốc điều trị CLE có liên quan đáng kể đến QoL cao hơn. KẾT LUẬN Các phát hiện của nghiên cứu hỗ trợ cho gánh nặng đáng kể liên quan đến CLE. Một số biến số có thể thay đổi như đau, mệt mỏi, hình ảnh cơ thể và hoạt động của bệnh có liên quan đến QoL. Do đó, các biện pháp can thiệp kết hợp các biến số này có thể làm giảm tác động tiêu cực đến cuộc sống QoL và cải thiện kết quả sức khỏe ở bệnh nhân CLE. Hơn nữa, do tình trạng bệnh mãn tính và tái phát, cần có các chiến lược tập trung vào việc cải thiện QoL cho nhóm dân số dễ bị tổn thương này."} +{"idx": 1880073, "en": "INTRODUCTION Prognostic benefits of zofenopril over ramipril in the early phase of acute myocardial infarction have been reported by the SMILE study, but these benefits have not been tested in clinical practice in the Chinese population. The objective of this study was to compare the effectiveness and safety of zofenopril plus aspirin against ramipril plus aspirin in patients with acute myocardial infarction. METHODS Patients in the early phase of acute myocardial infarction received 30 mg zofenopril (ZF cohort, N=191) or 5 mg ramipril (RP cohort, N=256) b.i.d. plus 100 mg aspirin/day. Data regarding hospitalisation for cardiovascular disease, non-cardiovascular events and mortality were collected and analysed. RESULTS During 1 year of treatment, 47 (25%) patients in the ZF cohort and 97 (40%) patients in the RP cohort were hospitalised due to cardiovascular disease (p=0.002), and three (2%) patients in the ZF cohort and 14 (6%) patients in the RP cohort died (p=0.043). Lower incidences of dry cough (p=0.001) and anaemia (p=0.049) were reported in the ZF cohort. CONCLUSIONS The study recommends zofenopril with 100 mg aspirin for a longer period in patients with acute myocardial infarction with systolic dysfunction.", "gemini": "GIỚI THIỆU Nghiên cứu SMILE đã báo cáo những lợi ích về tiên lượng của zofenopril so với ramipril trong giai đoạn đầu của nhồi máu cơ tim cấp, nhưng những lợi ích này vẫn chưa được kiểm nghiệm trong thực hành lâm sàng ở dân số Trung Quốc. Mục tiêu của nghiên cứu này là so sánh hiệu quả và độ an toàn của zofenopril cộng với aspirin so với ramipril cộng với aspirin ở những bệnh nhân bị nhồi máu cơ tim cấp. PHƯƠNG PHÁP Những bệnh nhân trong giai đoạn đầu của nhồi máu cơ tim cấp đã được dùng 30 mg zofenopril (nhóm ZF, N = 191) hoặc 5 mg ramipril (nhóm RP, N = 256) hai lần/ngày cộng với 100 mg aspirin/ngày. Dữ liệu về tình trạng nhập viện do bệnh tim mạch, các biến cố không phải tim mạch và tử vong đã được thu thập và phân tích. KẾT QUẢ Trong 1 năm điều trị, 47 (25%) bệnh nhân trong nhóm ZF và 97 (40%) bệnh nhân trong nhóm RP đã nhập viện do bệnh tim mạch (p = 0,002), và ba (2%) bệnh nhân trong nhóm ZF và 14 (6%) bệnh nhân trong nhóm RP đã tử vong (p = 0,043). Tỷ lệ ho khan thấp hơn (p = 0,001) và thiếu máu (p = 0,049) đã được báo cáo ở nhóm ZF. KẾT LUẬN Nghiên cứu này khuyến cáo nên dùng zofenopril với 100 mg aspirin trong thời gian dài hơn ở những bệnh nhân bị nhồi máu cơ tim cấp có rối loạn chức năng tâm thu."} +{"idx": 1880074, "en": "The practice of heading in soccer has become a public concern because of the potential for subconcussive impacts to cause cumulative concussive-like effects; however, experimental evidence for this hypothesis has been mixed. This systematic review used pre-defined search parameters to assess primary literature that examined changes in cognitive, behavioral, structural, and/or biological processes after acute heading exposure in youth and young adult soccer players. The findings were synthesized into a concise and comprehensive summary of the research following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) format, and suggestions for standardization of acute heading protocols are described. A total of 1189 articles were considered for this review, with 19 articles meeting all of the inclusion criteria for full analysis. An attempt was made to identify methods with significant sensitivity and reliability by grouping studies based on their outcome measures. Because of lack of standardization across intervention types and data collection protocols, no sensitive and reliable methods could be identified conclusively to assess the effects of acute heading exposure in soccer players. Based on this review, there is not enough evidence to either support or refute the potential of effects of subconcussive events from acute soccer heading exposure. Recommendations for standardization of acute heading exposure studies based on the included literature are discussed. Standardization is required to better understand the impact of acute heading exposure in soccer players, while allowing for the development of guidelines that mitigate any potential risks and allowing athletes to remain active and develop their skills.", "gemini": "Việc thực hành đánh đầu trong bóng đá đã trở thành mối quan tâm của công chúng vì khả năng tác động dưới ngưỡng chấn động gây ra các tác động giống như chấn động cộng dồn; tuy nhiên, bằng chứng thực nghiệm cho giả thuyết này vẫn còn nhiều. Đánh giá có hệ thống này đã sử dụng các thông số tìm kiếm được xác định trước để đánh giá các tài liệu chính đã kiểm tra những thay đổi về quá trình nhận thức, hành vi, cấu trúc và/hoặc sinh học sau khi tiếp xúc với đánh đầu cấp tính ở cầu thủ bóng đá trẻ và thanh thiếu niên. Các phát hiện đã được tổng hợp thành một bản tóm tắt ngắn gọn và toàn diện về nghiên cứu theo định dạng các mục báo cáo ưa thích cho các đánh giá có hệ thống và phân tích tổng hợp (PRISMA) và các đề xuất về tiêu chuẩn hóa các giao thức đánh đầu cấp tính được mô tả. Tổng cộng có 1189 bài báo đã được xem xét cho đánh giá này, với 19 bài báo đáp ứng tất cả các tiêu chuẩn đưa vào để phân tích đầy đủ. Người ta đã cố gắng xác định các phương pháp có độ nhạy và độ tin cậy đáng kể bằng cách nhóm các nghiên cứu dựa trên các biện pháp kết quả của họ. Do thiếu tiêu chuẩn hóa giữa các loại can thiệp và giao thức thu thập dữ liệu, không có phương pháp nhạy và đáng tin cậy nào có thể được xác định một cách dứt khoát để đánh giá các tác động của việc tiếp xúc với đánh đầu cấp tính ở cầu thủ bóng đá. Dựa trên đánh giá này, không có đủ bằng chứng để hỗ trợ hoặc bác bỏ khả năng xảy ra các tác động của các sự kiện dưới ngưỡng chấn động từ việc tiếp xúc với đánh đầu cấp tính trong bóng đá. Các khuyến nghị về tiêu chuẩn hóa các nghiên cứu tiếp xúc với đánh đầu cấp tính dựa trên các tài liệu đưa vào được thảo luận. Việc tiêu chuẩn hóa là cần thiết để hiểu rõ hơn về tác động của việc tiếp xúc với đánh đầu cấp tính ở cầu thủ bóng đá, đồng thời cho phép phát triển các hướng dẫn để giảm thiểu mọi rủi ro tiềm ẩn và cho phép các vận động viên tiếp tục hoạt động và phát triển các kỹ năng của mình."} +{"idx": 1880075, "en": "BACKGROUND Arteriovenous fistulae (AVF) are preferred for dialysis access but require accurate cannulation for effective dialysis. Evidence supports improvements in cannulation and complication rates using ultrasound guidance (USG) in cannulating other sites. This mixed methods, randomised controlled trial aimed to assess effects of USG during AVF cannulation. METHODS Participants with difficult to cannulate AVF had each cannulation event randomised to USG or standard technique (no USG). The primary outcome was the incidence and number of additional needle passes. Secondary outcomes included: the incidence and number of additional skin punctures; time to achieve two needle cannulation; pain associated with cannulation; local complications. Qualitative outcomes were assessed using patient and staff questionnaires. RESULTS Thirty-two participants had 346 cannulation events randomised (170 to USG and 176 to standard cannulation). USG resulted in a significant reduction in additional needle passes (72 vs 99 p = 0.007) and additional skin punctures (10 vs 25 p = 0.016.) but prolonged time to cannulation (p > 0.001). There was no difference in pain score (p = 0.705) or complications between groups. Questionnaires demonstrated that USG cannulation is acceptable to patients and staff. CONCLUSION USG cannulation of AVF is more accurate and no more painful than non-image guided cannulation, but prolonged time to cannulation. Some of the excess time involved may be due to the trial being performed early in cannulating staff's learning curve with the USG technique. Further work to elucidate which patients gain most benefit from USG cannulation and the effect of USG on cannulation complications and AVF patency is warranted.", "gemini": "BỐI CẢNH Các lỗ rò động mạch tĩnh mạch (AVF) được ưu tiên sử dụng để tiếp cận thẩm phân nhưng đòi hỏi phải luồn kim chính xác để thẩm phân hiệu quả. Bằng chứng ủng hộ việc cải thiện tỷ lệ luồn kim và biến chứng bằng cách sử dụng hướng dẫn siêu âm (USG) khi luồn kim vào các vị trí khác. Thử nghiệm đối chứng ngẫu nhiên, phương pháp hỗn hợp này nhằm đánh giá hiệu quả của USG trong quá trình luồn kim vào AVF. PHƯƠNG PHÁP Những người tham gia có AVF khó luồn kim đã được ngẫu nhiên đưa vào mỗi sự kiện luồn kim để sử dụng USG hoặc kỹ thuật tiêu chuẩn (không có USG). Kết quả chính là tỷ lệ mắc và số lần luồn kim bổ sung. Các kết quả phụ bao gồm: tỷ lệ mắc và số lần chọc da bổ sung; thời gian để đạt được hai lần luồn kim; đau liên quan đến việc luồn kim; các biến chứng tại chỗ. Các kết quả định tính được đánh giá bằng cách sử dụng bảng câu hỏi của bệnh nhân và nhân viên. KẾT QUẢ Ba mươi hai người tham gia có 346 sự kiện luồn kim được ngẫu nhiên (170 cho USG và 176 cho luồn kim tiêu chuẩn). USG dẫn đến giảm đáng kể số lần luồn kim bổ sung (72 so với 99 p = 0,007) và số lần chọc da bổ sung (10 so với 25 p = 0,016) nhưng kéo dài thời gian luồn kim (p> 0,001). Không có sự khác biệt về điểm đau (p = 0,705) hoặc biến chứng giữa các nhóm. Các bảng câu hỏi chứng minh rằng việc luồn kim bằng USG được bệnh nhân và nhân viên chấp nhận. KẾT LUẬN Việc luồn kim bằng USG vào AVF chính xác hơn và không đau hơn so với việc luồn kim không có hình ảnh hướng dẫn, nhưng kéo dài thời gian luồn kim. Một số thời gian thừa có thể là do thử nghiệm được thực hiện vào giai đoạn đầu trong đường cong học tập của nhân viên luồn kim với kỹ thuật USG. Cần có thêm nghiên cứu để làm sáng tỏ những bệnh nhân nào được hưởng lợi nhiều nhất từ ​​việc luồn kim bằng USG và tác dụng của USG đối với các biến chứng luồn kim và tình trạng thông của AVF."} +{"idx": 1880076, "en": "Although childhood traumatic brain injury (TBI) has been linked to heightened risk of impaired social skills and behavior, current evidence is weakened by small studies of variable methodological quality. To address these weaknesses, this international multi-cohort study involved synthesis of data from two large observational cohort studies of complicated mild-severe child TBI in Australia and North America. Both studies adopted a unified approach to data collection and coding procedures, providing the opportunity to merge datasets from multiple, well-characterized cohorts for which gold standard measures of social outcomes were collected during the chronic recovery phase. The study involved 218 children, including 33 children with severe TBI, 83 children with complicated mild-moderate TBI, 59 children with orthopedic injury, and 43 age- and sex-matched typically developing control children. All injured children were recruited from academic children's hospitals and underwent direct cognitive assessments including measures of theory of mind (ToM) at least 1-year post- injury. Parents rated their child's social adjustment using standardized measures of social skills, communication and behavior. Results showed a brain-injury specific effect on ToM abilities, such that children with both complicated mild to moderate and severe TBI displayed significantly poorer ToM than children without TBI. In mediator models, poorer ToM predicted poorer parent-rated self-direction and social skills, as well as more frequent behavioral symptoms. The ToM mediated the effect of severe TBI on parent ratings of communication and social skills, as well as on overall behavior symptoms. The findings suggest that deficits in ToM are evident across the spectrum of TBI severity and represent one mechanism linking severe child TBI to long-term social adjustment difficulties. The findings underscore the value of large-scale data harmonization projects to increase the quality of evidence regarding the outcomes of TBI. Clinical and scientific implications are discussed.", "gemini": "Mặc dù chấn thương sọ não (TBI) ở trẻ em có liên quan đến nguy cơ cao về các kỹ năng xã hội và hành vi bị suy yếu, nhưng bằng chứng hiện tại bị suy yếu do các nghiên cứu nhỏ có chất lượng phương pháp khác nhau. Để giải quyết những điểm yếu này, nghiên cứu đa nhóm quốc tế này liên quan đến việc tổng hợp dữ liệu từ hai nghiên cứu nhóm quan sát lớn về TBI ở trẻ em từ nhẹ đến nặng phức tạp ở Úc và Bắc Mỹ. Cả hai nghiên cứu đều áp dụng cách tiếp cận thống nhất đối với các thủ tục thu thập và mã hóa dữ liệu, tạo cơ hội hợp nhất các bộ dữ liệu từ nhiều nhóm đối chứng được mô tả rõ ràng, trong đó các biện pháp chuẩn vàng về kết quả xã hội đã được thu thập trong giai đoạn phục hồi mãn tính. Nghiên cứu này bao gồm 218 trẻ em, trong đó có 33 trẻ bị TBI nặng, 83 trẻ bị TBI nhẹ đến trung bình phức tạp, 59 trẻ bị thương chỉnh hình và 43 trẻ đối chứng phát triển bình thường được ghép đôi về tuổi và giới tính. Tất cả trẻ em bị thương đều được tuyển dụng từ các bệnh viện nhi khoa học thuật và trải qua các đánh giá nhận thức trực tiếp bao gồm các biện pháp về lý thuyết trí óc (ToM) ít nhất 1 năm sau khi bị thương. Cha mẹ đánh giá sự điều chỉnh xã hội của con mình bằng các biện pháp chuẩn hóa về kỹ năng xã hội, giao tiếp và hành vi. Kết quả cho thấy tác động cụ thể của chấn thương não đối với các khả năng của ToM, chẳng hạn như trẻ em bị cả TBI nhẹ đến trung bình và nặng phức tạp đều biểu hiện ToM kém hơn đáng kể so với trẻ em không bị TBI. Trong các mô hình trung gian, ToM kém hơn dự đoán khả năng tự định hướng và các kỹ năng xã hội kém hơn do cha mẹ đánh giá, cũng như các triệu chứng hành vi thường xuyên hơn. ToM đã làm trung gian cho tác động của TBI nặng đối với các đánh giá của cha mẹ về giao tiếp và kỹ năng xã hội, cũng như đối với các triệu chứng hành vi nói chung. Những phát hiện này cho thấy rằng các khiếm khuyết trong ToM là rõ ràng trên toàn bộ mức độ nghiêm trọng của TBI và đại diện cho một cơ chế liên kết TBI nghiêm trọng ở trẻ em với những khó khăn trong điều chỉnh xã hội lâu dài. Những phát hiện này nhấn mạnh giá trị của các dự án hài hòa dữ liệu quy mô lớn để tăng chất lượng bằng chứng liên quan đến kết quả của TBI. Các tác động lâm sàng và khoa học được thảo luận."} +{"idx": 1880077, "en": "Macrophage activation syndrome (MAS) is a form of secondary hemophagocytic lymphohistiocytosis and is a rapidly progressive, life-threatening complication of adult-onset Still's disease (AOSD). An anti-IL-6 receptor monoclonal antibody, tocilizumab, has shown to be effective in the treatment of AOSD but may precipitate MAS in patients with AOSD. The precise mechanism of MAS developed during anti-cytokine biologic agents remains unknown, but selective inhibition of a subset of pathways could impact other immune signalling pathways and trigger MAS. We herein describe a case of AOSD with the opposite outcomes of tocilizumab therapy, remission and development of MAS, after tocilizumab treatment at the initial flare and the relapse. From the comparison of clinical characteristics and concomitant treatment around the time of starting tocilizumab in both flares, the type and intensity of concomitant immunosuppressive therapy might strongly affect MAS development during tocilizumab therapy.", "gemini": "Hội chứng hoạt hóa đại thực bào (MAS) là một dạng của bệnh tăng sinh tế bào lympho mô cầu thực bào thứ phát và là một biến chứng đe dọa tính mạng tiến triển nhanh của bệnh Still khởi phát ở người lớn (AOSD). Một kháng thể đơn dòng thụ thể anti-IL-6, tocilizumab, đã được chứng minh là có hiệu quả trong điều trị AOSD nhưng có thể gây ra MAS ở những bệnh nhân mắc AOSD. Cơ chế chính xác của MAS phát triển trong quá trình sử dụng các tác nhân sinh học chống cytokine vẫn chưa được biết, nhưng việc ức chế chọn lọc một nhóm các con đường có thể ảnh hưởng đến các con đường truyền tín hiệu miễn dịch khác và gây ra MAS. Chúng tôi ở đây mô tả một trường hợp AOSD với các kết cục ngược nhau của liệu pháp tocilizumab, thuyên giảm và phát triển MAS, sau khi điều trị bằng tocilizumab ở lần bùng phát đầu tiên và tái phát. Từ việc so sánh các đặc điểm lâm sàng và điều trị đồng thời xung quanh thời điểm bắt đầu dùng tocilizumab ở cả hai đợt bùng phát, loại và cường độ của liệu pháp ức chế miễn dịch đồng thời có thể ảnh hưởng mạnh đến sự phát triển của MAS trong quá trình điều trị bằng tocilizumab."} +{"idx": 1880078, "en": "Many psychiatric populations present deficits in the processing of neutral faces. While insomnia is associated with perceptual alterations of facially expressed fear, sadness, and happiness, the perception of neutral faces have not been explored in this population. This study compared normal sleepers and individuals experiencing insomnia symptoms in their expression intensity ratings of neutral faces. A total of 56 normal sleepers scoring <5 on the Insomnia Severity Index and 58 individuals experiencing clinically significant insomnia symptoms scoring ≥15 on the Insomnia Severity Index (19.24 ± 3.53) observed 12 neutral facial photographs from the Karolinska Directed Emotional Faces database. Participants rated the extent to which each face appeared as attractive, sad, happy, trustworthy, approachable, healthy, and sociable. The results revelated a main effect of group, F(1,117) = 4.04, p = .047, and expression, F(7, 819) = 39.08, p = .001, on intensity ratings. While no significant Group × Expression interaction was confirmed, F(7,819)=1.03, p = .41, simple effects analysis determined that those experiencing insomnia symptoms rated neutral faces as significantly more attractive (34.30 ± 14.82; t(117) = -2.73, p = .007; Cohen's d = 0.50) and happy (34.83 ± 13.87; t(117) = -2.23, p = .028; Cohen's d = 0.41) compared with normal sleepers (Attractive: 26.89 ± 14.76; Happy: 28.90 ± 12.48). The present outcomes tentatively suggest that individuals experiencing clinically significant insomnia symptoms differentially perceive neutral faces when compared with normal sleepers. These outcomes present potentially negative psychosocial implications for those with insomnia.", "gemini": "Nhiều quần thể tâm thần biểu hiện sự thiếu hụt trong quá trình xử lý khuôn mặt trung tính. Trong khi mất ngủ có liên quan đến những thay đổi về nhận thức đối với nỗi sợ hãi, nỗi buồn và niềm vui được thể hiện trên khuôn mặt, thì nhận thức về khuôn mặt trung tính vẫn chưa được khám phá trong quần thể này. Nghiên cứu này so sánh những người ngủ bình thường và những người có triệu chứng mất ngủ về mức độ cường độ biểu hiện của khuôn mặt trung tính. Tổng cộng có 56 người ngủ bình thường đạt điểm <5 trên Chỉ số mức độ mất ngủ và 58 người có triệu chứng mất ngủ đáng kể về mặt lâm sàng đạt điểm ≥15 trên Chỉ số mức độ mất ngủ (19,24 ± 3,53) đã quan sát 12 bức ảnh khuôn mặt trung tính từ cơ sở dữ liệu Karolinska Directed Emotional Faces. Những người tham gia đánh giá mức độ mà mỗi khuôn mặt xuất hiện hấp dẫn, buồn, vui, đáng tin cậy, dễ gần, khỏe mạnh và hòa đồng. Kết quả cho thấy hiệu ứng chính của nhóm, F(1,117) = 4,04, p = 0,047 và biểu hiện, F(7, 819) = 39,08, p = 0,001, về xếp hạng cường độ. Mặc dù không có tương tác Nhóm × Biểu hiện đáng kể nào được xác nhận, F(7,819) = 1,03, p = 0,41, nhưng phân tích hiệu ứng đơn giản xác định rằng những người có triệu chứng mất ngủ đánh giá khuôn mặt trung tính hấp dẫn hơn đáng kể (34,30 ± 14,82; t(117) = -2,73, p = 0,007; Cohen's d = 0,50) và vui vẻ (34,83 ± 13,87; t(117) = -2,23, p = 0,028; Cohen's d = 0,41) so với những người ngủ bình thường (Hấp dẫn: 26,89 ± 14,76; Hạnh phúc: 28,90 ± 12,48). Những kết quả hiện tại cho thấy rằng những người có triệu chứng mất ngủ đáng kể về mặt lâm sàng nhận thức khuôn mặt trung tính khác biệt so với những người ngủ bình thường. Những kết quả này cho thấy những tác động tiêu cực về mặt tâm lý xã hội đối với những người bị mất ngủ."} +{"idx": 1880079, "en": "Drug administration to experimental rodents is often invasive and stressful, thus reducing animal welfare and potentially confounding experimental results. Methods of oral drug delivery in which rodents cooperate voluntarily minimize stress, pain and morbidity. We herein describe a method for oral administration through voluntary intake of strawberry jam, developed for C57BL/6J mice. During a 3-day habituation period, animals were placed in individual cages once daily and presented with a drop of jam. Five days later, the jam was again offered with admixed drug. Mice ingested it in less than 5 min, with latency times below 1 min, confirming the suitability of the administration method.", "gemini": "Việc dùng thuốc cho động vật gặm nhấm thực nghiệm thường gây xâm lấn và căng thẳng, do đó làm giảm phúc lợi động vật và có khả năng làm sai lệch kết quả thí nghiệm. Các phương pháp đưa thuốc qua đường uống mà ở đó động vật gặm nhấm hợp tác tự nguyện sẽ giảm thiểu căng thẳng, đau đớn và bệnh tật. Chúng tôi tại đây mô tả một phương pháp dùng thuốc qua đường uống thông qua việc tự nguyện ăn mứt dâu tây, được phát triển cho chuột C57BL/6J. Trong thời gian làm quen kéo dài 3 ngày, những con vật được đặt trong lồng riêng một lần mỗi ngày và được cho ăn một giọt mứt. Năm ngày sau, mứt lại được cho ăn với thuốc trộn lẫn. Chuột ăn hết trong vòng chưa đầy 5 phút, với thời gian chờ dưới 1 phút, xác nhận tính phù hợp của phương pháp dùng thuốc."} +{"idx": 1880080, "en": "Anaerobic digestion is a versatile biotechnology that produces bioenergy, biogas, from wastewater. Biogas production and wastewater treatment can be optimized by associating substrates with complementary characteristics. In this context, the aim of this study was to evaluate the performance of the anaerobic co-digestion of different contents of landfill leachate and crude glycerol compared to the organic matter removal and specific biogas production, the effects of the factors (time, glycerol content and substrate/inoculum ratio) and their interactions on kinetic parameters of specific biogas production using the modified Gompertz model. A Central Composite Rotational Design (CCRD) was performed for the experimental variables: time (16.6, 20, 25, 30 and 33.4 days), glycerol content (0.43, 0.70, 1.10, 1.50 and 1.77%) and substrate/inoculum ratio (0.23, 0.30, 0.40, 0.50 and 0.57 g COD/g VSS). From the optimization, it was possible to maximize the efficiency of organic matter removal (90.15%) and specific biogas production (403.15 mL/g VSS) in the conditions of 33.2 days, glycerol content of 1.71% and substrate/inoculum ratio of 0.37 g COD/g VSS. Concerning the modified Gompertz model of the optimal condition performed, an average of 20.3 times higher specific biogas production was obtained when compared to the monodigestion of leachate. Therefore, the association of leachate and glycerol was found to be feasible in terms of stability, biodegradability and biogas production.", "gemini": "Tiêu hóa kỵ khí là một công nghệ sinh học linh hoạt sản xuất năng lượng sinh học, khí sinh học, từ nước thải. Sản xuất khí sinh học và xử lý nước thải có thể được tối ưu hóa bằng cách kết hợp các chất nền có đặc tính bổ sung. Trong bối cảnh này, mục đích của nghiên cứu này là đánh giá hiệu suất của quá trình đồng tiêu hóa kỵ khí các chất thải rỉ rác và glycerol thô khác nhau so với quá trình loại bỏ chất hữu cơ và sản xuất khí sinh học cụ thể, các tác động của các yếu tố (thời gian, hàm lượng glycerol và tỷ lệ chất nền/chủng cấy) và tương tác của chúng đối với các thông số động học của sản xuất khí sinh học cụ thể bằng cách sử dụng mô hình Gompertz đã sửa đổi. Thiết kế quay hỗn hợp trung tâm (CCRD) đã được thực hiện cho các biến thí nghiệm: thời gian (16,6, 20, 25, 30 và 33,4 ngày), hàm lượng glycerol (0,43, 0,70, 1,10, 1,50 và 1,77%) và tỷ lệ chất nền/chủng cấy (0,23, 0,30, 0,40, 0,50 và 0,57 g COD/g VSS). Từ quá trình tối ưu hóa, có thể tối đa hóa hiệu suất loại bỏ chất hữu cơ (90,15%) và sản xuất khí sinh học cụ thể (403,15 mL/g VSS) trong điều kiện 33,2 ngày, hàm lượng glycerol 1,71% và tỷ lệ chất nền/chủng cấy 0,37 g COD/g VSS. Liên quan đến mô hình Gompertz đã sửa đổi của điều kiện tối ưu được thực hiện, sản lượng khí sinh học cụ thể trung bình cao hơn 20,3 lần so với quá trình tiêu hóa đơn lẻ của nước rỉ rác. Do đó, sự kết hợp giữa nước rỉ rác và glycerol được thấy là khả thi về mặt độ ổn định, khả năng phân hủy sinh học và sản xuất khí sinh học."} +{"idx": 1880081, "en": "BACKGROUND to identify the potential cardiovascular risk factors associated with mortality in hospitalised COVID-19 patients. METHODS All consecutive patients admitted to intensive care unit (ICU) of our institute for COVID-19 from 1 April 2020 to 20 May 2020 were included. Patient characteristics including complete medical history and comorbid diseases, admission and 7th day blood test results and clinical characteristics were compared between survivors and non-survivors. RESULTS There were no significant difference between survivors and non-survivors regarding age, gender, and pre-existing coronary artery disease, hypertension, diabetes, heart failure, coronary artery bypass grafting surgery, percutaneous coronary intervention and coronary stenting. Admission D-dimer and NT-proBNP levels of non-survivors were significantly higher than survivors. CRP, procalcitonin, creatine kinase (CK) and troponin I levels on 7th day of admission were significantly higher in non-survivors compared to survivors. In addition, both admission and 7th day lymphocyte count were lower in non-survivors compared to that of the survivors. CRP declined from admission to 7th day of hospitalisation in survivors, whereas a median 6.75 mg/L increase was observed in non survivors. The peak and minimum CRP, procalcitonin and levels were significantly higher in non-survivors than survivors. The peak NT-proBNP level of non-survivors was also significantly higher than that of the survivors. Intubation, lower GFR values and higher NT-proBNP values were predictive for death. CONCLUSION The prothrombotic coagulopathy mediated by the endothelial interaction with SARS-CoV-2 may also have role in unfavourable prognosis in COVID-19. These readily available biomarkers might be useful in risk stratification of COVID-19 cases.", "gemini": "BỐI CẢNH để xác định các yếu tố nguy cơ tim mạch tiềm ẩn liên quan đến tử vong ở bệnh nhân COVID-19 nhập viện. PHƯƠNG PHÁP Tất cả các bệnh nhân liên tiếp nhập khoa chăm sóc đặc biệt (ICU) của viện chúng tôi vì COVID-19 từ ngày 1 tháng 4 năm 2020 đến ngày 20 tháng 5 năm 2020 đều được đưa vào nghiên cứu. Các đặc điểm của bệnh nhân bao gồm tiền sử bệnh đầy đủ và các bệnh đi kèm, kết quả xét nghiệm máu vào ngày nhập viện và ngày thứ 7 và các đặc điểm lâm sàng đã được so sánh giữa những bệnh nhân sống sót và không sống sót. KẾT QUẢ Không có sự khác biệt đáng kể giữa những bệnh nhân sống sót và không sống sót về tuổi, giới tính, bệnh động mạch vành, tăng huyết áp, tiểu đường, suy tim, phẫu thuật bắc cầu động mạch vành, can thiệp động mạch vành qua da và đặt stent động mạch vành trước đó. Nồng độ D-dimer và NT-proBNP khi nhập viện của những bệnh nhân không sống sót cao hơn đáng kể so với những bệnh nhân sống sót. Nồng độ CRP, procalcitonin, creatine kinase (CK) và troponin I vào ngày thứ 7 nhập viện cao hơn đáng kể ở những bệnh nhân không sống sót so với những bệnh nhân sống sót. Ngoài ra, số lượng tế bào lympho khi nhập viện và ngày thứ 7 đều thấp hơn ở những bệnh nhân không sống sót so với những bệnh nhân sống sót. CRP giảm từ khi nhập viện đến ngày thứ 7 nhập viện ở những bệnh nhân sống sót, trong khi ở những bệnh nhân không sống sót, người ta quan sát thấy mức tăng trung bình là 6,75 mg/L. Nồng độ đỉnh và tối thiểu của CRP, procalcitonin và cao hơn đáng kể ở những bệnh nhân không sống sót so với những bệnh nhân sống sót. Nồng độ NT-proBNP đỉnh của những bệnh nhân không sống sót cũng cao hơn đáng kể so với những bệnh nhân sống sót. Đặt nội khí quản, giá trị GFR thấp hơn và giá trị NT-proBNP cao hơn là yếu tố tiên đoán tử vong. KẾT LUẬN Bệnh lý đông máu do tăng đông được trung gian bởi sự tương tác giữa nội mô với SARS-CoV-2 cũng có thể đóng vai trò trong tiên lượng xấu ở bệnh nhân COVID-19. Những dấu ấn sinh học dễ kiếm này có thể hữu ích trong việc phân tầng rủi ro của các trường hợp COVID-19."} +{"idx": 1880082, "en": "AIMS Our aim was to investigate labor-force participation, working hours, job changes, and education over 9 years among persons who have survived more than 10 years after cancer, and compare it to controls. METHODS Register data on 2629 persons who survived cancer were stratified by gender and compared to data on 5258 matched controls. Persons who survived cancer were aged 30-50 when diagnosed with cancer and had a work contract prior to diagnosis. Descriptive analysis and t-tests were performed. RESULTS The proportion of female persons who survived cancer in the labor force was reduced from 100% to 83.9% during follow-up, demonstrating a significant difference compared to controls for each year measured. The proportion of male persons who survived cancer dropped from 100% to 84.8%, but was only significantly different compared to controls in 2 years. The proportion of female persons who had survived cancer who worked full-time was lower in all years compared to both controls and male persons who survived cancer; in turn, male persons who had survived cancer worked full-time less than male controls. The proportion of female persons who had survived cancer who worked less than 20 hours per week increased compared to controls. The frequency of change of employer was higher among female persons who survived cancer compared to controls for some years, but no significant differences between male persons who survived cancer and controls were found. Female persons who survived cancer were in education more often than male persons who survived cancer. CONCLUSIONS Persons who survived cancer experienced reduced labor-force participation and working hours 9 years after diagnosis, and the reduction was more pronounced for women than for men. Working patterns were also different between genders and between persons who survived cancer and controls.", "gemini": "MỤC ĐÍCH Mục đích của chúng tôi là nghiên cứu sự tham gia lực lượng lao động, giờ làm việc, thay đổi công việc và giáo dục trong 9 năm ở những người đã sống sót sau hơn 10 năm sau khi mắc bệnh ung thư và so sánh với nhóm đối chứng. PHƯƠNG PHÁP Dữ liệu đăng ký của 2629 người sống sót sau ung thư được phân tầng theo giới tính và so sánh với dữ liệu của 5258 đối chứng được ghép nối. Những người sống sót sau ung thư có độ tuổi từ 30-50 khi được chẩn đoán mắc bệnh ung thư và có hợp đồng lao động trước khi được chẩn đoán. Phân tích mô tả và kiểm định t đã được thực hiện. KẾT QUẢ Tỷ lệ những người sống sót sau ung thư tham gia lực lượng lao động giảm từ 100% xuống 83,9% trong thời gian theo dõi, cho thấy sự khác biệt đáng kể so với nhóm đối chứng trong mỗi năm được đo lường. Tỷ lệ những người đàn ông sống sót sau ung thư giảm từ 100% xuống 84,8%, nhưng chỉ khác biệt đáng kể so với nhóm đối chứng trong 2 năm. Tỷ lệ những người phụ nữ sống sót sau ung thư làm việc toàn thời gian thấp hơn trong tất cả các năm so với cả nhóm đối chứng và những người đàn ông sống sót sau ung thư; ngược lại, những người đàn ông sống sót sau ung thư làm việc toàn thời gian ít hơn những người đàn ông trong nhóm đối chứng. Tỷ lệ những người phụ nữ sống sót sau ung thư làm việc dưới 20 giờ một tuần tăng so với nhóm đối chứng. Tần suất thay đổi người sử dụng lao động cao hơn ở những người phụ nữ sống sót sau ung thư so với nhóm đối chứng trong một số năm, nhưng không tìm thấy sự khác biệt đáng kể giữa những người đàn ông sống sót sau ung thư và nhóm đối chứng. Những người phụ nữ sống sót sau ung thư thường xuyên được giáo dục hơn những người đàn ông sống sót sau ung thư. KẾT LUẬN Những người sống sót sau ung thư có tỷ lệ tham gia lực lượng lao động và giờ làm việc giảm sau 9 năm kể từ khi được chẩn đoán và sự giảm này rõ rệt hơn ở phụ nữ so với nam giới. Mô hình làm việc cũng khác nhau giữa các giới tính và giữa những người sống sót sau ung thư và nhóm đối chứng."} +{"idx": 1880083, "en": "The consistently high rate of sexually transmitted infections among people 18-24 years old warrants novel strategies promoting condom use among large segments of this population. Ample opportunity exits to employ such strategies in university settings. This study evaluated a sex-positive, home-practice intervention designed to promote condom use among university undergraduates engaging in penile-vaginal intercourse (PVI). A sample of 29 opposite-sex undergraduate couples, engaging in PVI, was recruited from a large university. After teaching couples about condom use, a health educator helped couples select condoms/lubricants from options comprising various sizes, fits, shapes, textures, and other features. A 30-day 'homework assignment' was made to use these products while reducing condom use errors/problems and enhancing sexual pleasure. A repeated measures design, with a six-month observation period, was used to evaluate the program. Over the six-month period, the mean frequency of condomless PVI decreased from 26.4 to 3.9 times (P < .001). Significant and favorable changes were also found relative to attitudes toward condom use (P = .027) and in condom use self-efficacy (P < .001). Most students completing the follow-up assessment indicated the intervention would favorably impact their future condom use. Findings suggest this intervention program may be important to promoting condom use among university couples engaging in PVI.", "gemini": "Tỷ lệ nhiễm trùng lây truyền qua đường tình dục liên tục ở những người từ 18-24 tuổi đòi hỏi phải có các chiến lược mới thúc đẩy sử dụng bao cao su trong các phân khúc lớn của nhóm dân số này. Có nhiều cơ hội để sử dụng các chiến lược như vậy trong môi trường đại học. Nghiên cứu này đã đánh giá một biện pháp can thiệp thực hành tại nhà, tích cực về tình dục được thiết kế để thúc đẩy sử dụng bao cao su ở những sinh viên đại học tham gia quan hệ tình dục âm đạo-dương vật (PVI). Một mẫu gồm 29 cặp đôi khác giới, đang tham gia PVI, đã được tuyển dụng từ một trường đại học lớn. Sau khi hướng dẫn các cặp đôi về cách sử dụng bao cao su, một nhà giáo dục sức khỏe đã giúp các cặp đôi lựa chọn bao cao su/chất bôi trơn từ các lựa chọn bao gồm nhiều kích cỡ, kiểu dáng, hình dạng, kết cấu và các đặc điểm khác nhau. Một 'bài tập về nhà' trong 30 ngày đã được đưa ra để sử dụng các sản phẩm này trong khi giảm lỗi/vấn đề sử dụng bao cao su và tăng khoái cảm tình dục. Một thiết kế biện pháp lặp lại, với thời gian quan sát sáu tháng, đã được sử dụng để đánh giá chương trình. Trong giai đoạn sáu tháng, tần suất trung bình của PVI không dùng bao cao su đã giảm từ 26,4 xuống còn 3,9 lần (P <0,001). Những thay đổi đáng kể và thuận lợi cũng được tìm thấy liên quan đến thái độ đối với việc sử dụng bao cao su (P = 0,027) và khả năng tự sử dụng bao cao su (P <0,001). Hầu hết sinh viên hoàn thành đánh giá theo dõi cho biết rằng biện pháp can thiệp sẽ tác động thuận lợi đến việc sử dụng bao cao su trong tương lai của họ. Những phát hiện cho thấy chương trình can thiệp này có thể quan trọng để thúc đẩy sử dụng bao cao su ở các cặp đôi đại học tham gia PVI."} +{"idx": 1880084, "en": "In addition to being the epicentre of the HIV epidemic, South Africa also has the highest burden of sexually transmitted infections (STIs) in the world. Therefore, understanding the most influential risk factors of STIs is a research priority. Using the data from 9948 women who resided in KwaZulu Natal, South Africa, we estimated the population attributable risk to quantify the combined impacts of the most influential factors on STI diagnosis. Overall STI prevalence was 20%, and STI incidence was 15 per 100 person-years. Four factors: age at sexual debut, single/not cohabiting, two or more sex partners and parity <3 were identified as the most influential risk factors for STI prevalence and incidence rates. However, these factors collectively associated with only 51% and 53% of the excess STI prevalence and incidence rates, respectively. These relatively modest impacts provide empirical evidence for the significant impacts of unmeasured factors on STIs. Culturally and socially appropriate prevention programs may be more effective to target those at highest risk of STIs.", "gemini": "Ngoài việc là tâm dịch của đại dịch HIV, Nam Phi còn có gánh nặng nhiễm trùng lây truyền qua đường tình dục (STI) cao nhất thế giới. Do đó, việc hiểu được các yếu tố nguy cơ có ảnh hưởng nhất đến STI là một ưu tiên nghiên cứu. Sử dụng dữ liệu từ 9948 phụ nữ cư trú tại KwaZulu Natal, Nam Phi, chúng tôi đã ước tính nguy cơ có thể quy cho dân số để định lượng các tác động kết hợp của các yếu tố có ảnh hưởng nhất đến chẩn đoán STI. Tỷ lệ mắc STI chung là 20% và tỷ lệ mắc STI là 15 trên 100 người-năm. Bốn yếu tố: tuổi bắt đầu quan hệ tình dục, độc thân/không chung sống, có hai hoặc nhiều bạn tình và sinh con <3 được xác định là các yếu tố nguy cơ có ảnh hưởng nhất đến tỷ lệ mắc STI và tỷ lệ mắc. Tuy nhiên, các yếu tố này kết hợp với nhau chỉ liên quan đến 51% và 53% tỷ lệ mắc STI và tỷ lệ mắc STI tương ứng. Những tác động tương đối khiêm tốn này cung cấp bằng chứng thực nghiệm cho thấy tác động đáng kể của các yếu tố không được đo lường đối với STI. Các chương trình phòng ngừa phù hợp về mặt văn hóa và xã hội có thể hiệu quả hơn trong việc nhắm mục tiêu vào những người có nguy cơ mắc STI cao nhất."} +{"idx": 1880085, "en": "The rapid spread of the COVID-19 virus around the world poses a real threat to public safety. Some COVID-19 symptoms are similar to other viral chest diseases, which makes it challenging to develop models for effective detection of COVID-19 infection. This article advocates a model to differentiate between COVID-19 and other four viral chest diseases under uncertainty environment using the viruses primary symptoms and CT scans. The proposed model is based on a plithogenic set, which provides higher accurate evaluation results in an uncertain environment. The proposed model employs the best-worst method (BWM) and the technique in order of preference by similarity to ideal solution (TOPSIS). Besides, this study discusses how smart Internet of Things technology can assist medical staff in monitoring the spread of COVID-19. Experimental evaluation of the proposed model was conducted on five different chest diseases. Evaluation results demonstrate that the proposed model effectiveness in detecting the COVID-19 in all five cases achieving detection accuracy of up to 98%.", "gemini": "Sự lây lan nhanh chóng của vi-rút COVID-19 trên toàn thế giới gây ra mối đe dọa thực sự đối với an toàn công cộng. Một số triệu chứng của COVID-19 tương tự như các bệnh về ngực do vi-rút khác, khiến việc xây dựng các mô hình để phát hiện hiệu quả tình trạng nhiễm COVID-19 trở nên khó khăn. Bài báo này ủng hộ một mô hình để phân biệt giữa COVID-19 và bốn bệnh về ngực do vi-rút khác trong môi trường không chắc chắn bằng cách sử dụng các triệu chứng chính của vi-rút và chụp CT. Mô hình được đề xuất dựa trên một tập hợp plithogenic, cung cấp kết quả đánh giá chính xác hơn trong môi trường không chắc chắn. Mô hình được đề xuất sử dụng phương pháp tốt nhất-tệ nhất (BWM) và kỹ thuật theo thứ tự ưu tiên theo giải pháp lý tưởng (TOPSIS). Bên cạnh đó, nghiên cứu này thảo luận về cách công nghệ Internet of Things thông minh có thể hỗ trợ đội ngũ y tế trong việc theo dõi sự lây lan của COVID-19. Đánh giá thực nghiệm mô hình được đề xuất đã được tiến hành trên năm bệnh về ngực khác nhau. Kết quả đánh giá chứng minh rằng mô hình được đề xuất có hiệu quả trong việc phát hiện COVID-19 trong cả năm trường hợp, đạt độ chính xác phát hiện lên đến 98%."} +{"idx": 1880086, "en": "AIMS There are well-known gender differences in smoking, including the pattern of use and the effectiveness of smoking prevention programs. However, little is known about the differences between boys and girls in their attitudes towards smoking prevention interventions. This study explores gender differences in attitudes towards a school-based intervention to prevent smoking. METHODS We used data from the X:IT II intervention study conducted in 46 Danish elementary schools. RESULTS Compared to boys, girls were more positive towards smoke-free school time, both concerning rules for teachers smoking (odds ratio (OR) = 1.69, 95% confidence interval (CI): 1.35-2.12) and for students smoking (OR = 1.41, 95% CI: 1.13-1.76). No difference was observed in students signing the smoke-free agreement. However, a larger proportion of girls reported that the agreement was a good occasion to talk about smoking with their parents (OR = 1.36, 95% CI: 1.13-1.76). Girls were also more positive towards the smoke-free curriculum (OR = 1.52, 95% CI: 1.19-1.94). CONCLUSIONS This study showed that girls were, overall, more positive towards the components of the smoking preventive intervention. Our findings highlight the importance of considering differences in intervention preferences for boys and girls in future health prevention initiatives.", "gemini": "MỤC TIÊU Có sự khác biệt rõ rệt về giới trong việc hút thuốc, bao gồm cả kiểu sử dụng và hiệu quả của các chương trình phòng ngừa hút thuốc. Tuy nhiên, người ta biết rất ít về sự khác biệt giữa bé trai và bé gái trong thái độ của các em đối với các biện pháp can thiệp phòng ngừa hút thuốc. Nghiên cứu này khám phá sự khác biệt về giới trong thái độ đối với một biện pháp can thiệp tại trường học nhằm ngăn ngừa hút thuốc. PHƯƠNG PHÁP Chúng tôi đã sử dụng dữ liệu từ nghiên cứu can thiệp X:IT II được tiến hành tại 46 trường tiểu học của Đan Mạch. KẾT QUẢ So với bé trai, bé gái có thái độ tích cực hơn đối với thời gian học không khói thuốc, cả về quy định đối với giáo viên hút thuốc (tỷ lệ chênh lệch (OR) = 1,69, khoảng tin cậy 95% (CI): 1,35-2,12) và đối với học sinh hút thuốc (OR = 1,41, 95% CI: 1,13-1,76). Không có sự khác biệt nào được quan sát thấy ở học sinh ký thỏa thuận không khói thuốc. Tuy nhiên, một tỷ lệ lớn hơn các bé gái báo cáo rằng thỏa thuận là một dịp tốt để nói chuyện về việc hút thuốc với cha mẹ của các em (OR = 1,36, 95% CI: 1,13-1,76). Các bé gái cũng có thái độ tích cực hơn đối với chương trình giảng dạy không khói thuốc (OR = 1,52, 95% CI: 1,19-1,94). KẾT LUẬN Nghiên cứu này cho thấy rằng nhìn chung, các bé gái có thái độ tích cực hơn đối với các thành phần của biện pháp can thiệp phòng ngừa hút thuốc. Những phát hiện của chúng tôi nhấn mạnh tầm quan trọng của việc xem xét sự khác biệt trong sở thích can thiệp của bé trai và bé gái trong các sáng kiến phòng ngừa sức khỏe trong tương lai."} +{"idx": 1880087, "en": "Microtia is a congenital malformation of the auricle. The conventional therapy for microtia is reconstruction of the auricle by using the patient's own costal cartilage. Because it is invasive to harvest costal cartilages, less invasive ways for auricular reconstruction need to be established. Recent reports have indicated a new method for the production of cartilaginous particles from human induced pluripotent stem cells. To adopt this method to create an auricular-shaped regenerative cartilage, a novel scaffold with the property of a three-dimensional shape memory was created. A scaffold with a three-dimensional shape of auricular frames composed of a helix and an antihelix, which was designed to mimic an auricular framework carved from autologous costal cartilage and transplanted in auricular reconstruction, was prepared, filled with cartilaginous particles, and subcutaneously transplanted in nude rats. The auricular-shaped regenerative cartilage maintained the given shape and cartilage features in vivo for 1 year. Our findings suggest a novel approach for auricular reconstruction.", "gemini": "Việc ném bóng chính xác và với tốc độ tối ưu dựa trên sự tương tác phức tạp của nhiều biến số. Mặc dù sức mạnh và năng lượng đã được chứng minh là có liên quan đến tốc độ ném bóng ở các cầu thủ cricket, nhưng các cơ riêng lẻ góp phần vào chức năng tối ưu của phức hợp vai vẫn chưa được khám phá đầy đủ liên quan đến hiệu suất ném bóng. Do đó, nghiên cứu này nhằm mục đích tìm hiểu mối tương quan giữa các biến số cơ xương và hiệu suất ném bóng trên cao ở các cầu thủ cricket. Ba mươi hai cầu thủ cricket nam nghiệp dư đã được kiểm tra bằng một loạt 16 bài kiểm tra (sức mạnh, sự linh hoạt, vị trí xương bả vai) cũng như tốc độ ném bóng (TS) và bài kiểm tra độ chính xác mới (TA). Chỉ có hai trong số mười sáu bài kiểm tra có tương quan với hiệu suất ném bóng trong phân tích hồi quy bội. Sức mạnh bắt cóc hông không thuận tay tương quan thuận với TS (p < 0,05): trung bình, sức mạnh tăng 10 newton (N) tương ứng với sự gia tăng TS là 0,60 km/giờ (95% CI: 0,12-1,08). Chiều dài cơ ngực bé không thuận tay tương quan thuận với TA (p < 0,01): trung bình, chiều dài tăng một cm tương ứng với sự gia tăng 0,633 điểm (95% CI: 0,225-1,041). Nghiên cứu cắt ngang này chứng minh rằng trong số một loạt các biến số cơ xương, chỉ có sức mạnh bắt cóc hông không thuận tay tương quan với TS, trong khi chỉ có chiều dài cơ ngực bé không thuận tay tương quan với TA ở những người chơi cricket nghiệp dư."} +{"idx": 1880088, "en": "Objective: The Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) has three delayed recall subtests (list, story, figure), but only one delayed recognition subtest (list). Since comparisons between delayed recall and recognition can be useful in clinical neuropsychology, the current study sought to develop and preliminarily examine two proposed new subtests for Form A of the RBANS, Story Recognition and Figure Recognition. Method: A sample of older adults who were cognitively intact (n = 48) or classified with amnestic Mild Cognitive Impairment (MCI, n = 29) or mild Alzheimer's disease (AD, n = 24) were administered the RBANS and the two new recognition subtests. Results: In the primary analyses, cognitively intact participants performed significantly better than the two memory-impaired groups on all twelve scores (one recall and three recognition [total, hits, false positive errors] for the list, story, and figure). For amnestic MCI and AD participants, they showed statistically comparable scores on 7 of the 12 variables, where those with MCI performed better than those with AD on the other five scores. Across the three groups, effect sizes were large (e.g., Cohen's d = 1.0-2.9). In secondary analyses, all of the List Recall and Recognition scores significantly correlated with one another, and this pattern was observed for all of the Story Recall and Recognition scores and most of the Figure Recall and Recognition scores. Conclusions: Although preliminary, these new recognition scores appear to provide useful information and may improve the sensitivity of the RBANS in identifying cortical/subcortical profiles in clinical and research settings.", "gemini": "Mục tiêu: Thang đo lặp lại để đánh giá tình trạng thần kinh (RBANS) có ba tiểu bài kiểm tra nhớ lại chậm (danh sách, câu chuyện, hình ảnh), nhưng chỉ có một tiểu bài kiểm tra nhận dạng chậm (danh sách). Vì việc so sánh giữa việc nhớ lại chậm và nhận dạng chậm có thể hữu ích trong tâm lý học thần kinh lâm sàng, nên nghiên cứu hiện tại tìm cách phát triển và kiểm tra sơ bộ hai tiểu bài kiểm tra mới được đề xuất cho Biểu mẫu A của RBANS, Nhận dạng câu chuyện và Nhận dạng hình ảnh. Phương pháp: Một mẫu những người lớn tuổi nhận thức còn nguyên vẹn (n = 48) hoặc được phân loại mắc chứng suy giảm nhận thức nhẹ do mất trí nhớ (MCI, n = 29) hoặc bệnh Alzheimer nhẹ (AD, n = 24) được thực hiện RBANS và hai tiểu bài kiểm tra nhận dạng mới. Kết quả: Trong các phân tích chính, những người tham gia nhận thức còn nguyên vẹn thực hiện tốt hơn đáng kể so với hai nhóm suy giảm trí nhớ trên tất cả mười hai điểm (một điểm nhớ lại và ba điểm nhận dạng [tổng điểm, đúng, lỗi dương tính giả] cho danh sách, câu chuyện và hình ảnh). Đối với những người tham gia mắc chứng MCI và AD do mất trí nhớ, họ cho thấy điểm số tương đương về mặt thống kê trên 7 trong số 12 biến, trong đó những người mắc MCI thực hiện tốt hơn những người mắc AD trên năm điểm còn lại. Trong ba nhóm, quy mô hiệu ứng lớn (ví dụ: d của Cohen = 1,0-2,9). Trong các phân tích thứ cấp, tất cả các điểm Nhận dạng và Nhớ lại danh sách đều có tương quan đáng kể với nhau và mô hình này được quan sát thấy đối với tất cả các điểm Nhận dạng và Nhớ lại câu chuyện và hầu hết các điểm Nhận dạng và Nhớ lại hình ảnh. Kết luận: Mặc dù chỉ là sơ bộ, nhưng những điểm nhận dạng mới này dường như cung cấp thông tin hữu ích và có thể cải thiện độ nhạy của RBANS trong việc xác định hồ sơ vỏ não/dưới vỏ não trong các thiết lập lâm sàng và nghiên cứu."} +{"idx": 1880089, "en": "Optimal throwing speed and accuracy is built on a complex interaction of multiple variables. Although strength and power has been associated with throwing speed in cricketers, the individual muscles that contribute to optimal function of the shoulder-complex has not been adequately explored in connection with throwing performance. Consequently, this study aimed to investigate the correlation between musculoskeletal variables and overhead throwing performance in cricketers. Thirty-two amateur male cricketers were tested using a battery of 16 tests (strength, flexibility, scapula positioning) as well as a throwing speed (TS) and a novel accuracy test (TA). Only two of the sixteen tests were correlated with throwing performance in the multiple regression analysis. Non-dominant hip abduction strength correlated positively with TS (p < 0.05): on average, a strength increase of 10 newtons (N) was associated with an increase in TS of 0.60 km/h (95% CI: 0.12-1.08). Non-dominant pectoralis minor length correlated positively with TA (p < 0.01): on average, a one-centimetre increase in the length correlated to an increase, of 0.633 points (95% CI: 0.225-1.041). This cross-sectional study demonstrated that from an array of musculoskeletal variables, only non-dominant hip abduction strength correlated with TS, while only non-dominant pectoralis minor length correlated with TA in amateur cricketers.", "gemini": "Viêm tai giữa là một dị tật bẩm sinh của vành tai. Phương pháp điều trị thông thường đối với viêm tai giữa là tái tạo vành tai bằng cách sử dụng sụn sườn của chính bệnh nhân. Vì việc lấy sụn sườn là xâm lấn, nên cần thiết lập các phương pháp ít xâm lấn hơn để tái tạo vành tai. Các báo cáo gần đây đã chỉ ra một phương pháp mới để sản xuất các hạt sụn từ tế bào gốc đa năng cảm ứng của con người. Để áp dụng phương pháp này nhằm tạo ra sụn tái tạo hình vành tai, một loại khung xương mới có đặc tính nhớ hình ba chiều đã được tạo ra. Một khung xương có hình dạng ba chiều của khung vành tai bao gồm một xoắn ốc và một xoắn ốc chống, được thiết kế để mô phỏng một khung vành tai được chạm khắc từ sụn sườn tự thân và được ghép trong quá trình tái tạo vành tai, đã được chuẩn bị, chứa đầy các hạt sụn và được ghép dưới da ở chuột không lông. Sụn tái tạo hình vành tai duy trì hình dạng và các đặc điểm của sụn đã cho trong cơ thể sống trong 1 năm. Những phát hiện của chúng tôi gợi ý một cách tiếp cận mới để tái tạo vành tai."} +{"idx": 1880090, "en": "Mania, the core feature of bipolar disorder, is associated with heightened and positive emotion responding. Yet, little is known about the underlying cognitive processes that may contribute to heightened positive emotionality observed. Additionally, while previous research has investigated positive emotion biases in non-clinical samples, few if any, account for subthreshold clinical symptoms or traits, which have reliably assessed psychopathological risk. The present study compared continuous scores on a widely used self-report measure of hypomania proneness (HPS-48) with a dot-probe task to investigate attentional biases for happy, angry, fearful, and neutral faces among 66 college student participants. Results suggested that hypomania proneness was positively associated with attentional bias towards happy, but not angry or fearful faces. Results remained robust when controlling for positive affect and did not appear to be affected by negative affect or current symptoms of depression, anxiety, and stress. Findings provide insight into potential behavioural markers that co-occur with heightened positive emotional responding and hypomania in emerging adults.", "gemini": "Rối loạn lưỡng cực, đặc điểm cốt lõi là hưng cảm, có liên quan đến phản ứng cảm xúc tích cực và gia tăng. Tuy nhiên, người ta vẫn biết rất ít về các quá trình nhận thức cơ bản có thể góp phần vào cảm xúc tích cực gia tăng đã quan sát thấy. Ngoài ra, trong khi các nghiên cứu trước đây đã điều tra về sự thiên vị cảm xúc tích cực trong các mẫu không lâm sàng, thì rất ít hoặc không có nghiên cứu nào tính đến các triệu chứng hoặc đặc điểm lâm sàng dưới ngưỡng, vốn đã được đánh giá đáng tin cậy về nguy cơ bệnh lý tâm thần. Nghiên cứu hiện tại đã so sánh các điểm số liên tục trên một biện pháp tự báo cáo được sử dụng rộng rãi về khuynh hướng hưng nhẹ (HPS-48) với nhiệm vụ thăm dò chấm để điều tra sự thiên vị chú ý đối với khuôn mặt vui vẻ, tức giận, sợ hãi và trung tính trong số 66 sinh viên đại học tham gia. Kết quả cho thấy khuynh hướng hưng nhẹ có liên quan tích cực với sự thiên vị chú ý đối với khuôn mặt vui vẻ, nhưng không phải khuôn mặt tức giận hoặc sợ hãi. Kết quả vẫn mạnh mẽ khi kiểm soát cảm xúc tích cực và dường như không bị ảnh hưởng bởi cảm xúc tiêu cực hoặc các triệu chứng hiện tại của trầm cảm, lo âu và căng thẳng. Những phát hiện này cung cấp cái nhìn sâu sắc về các dấu hiệu hành vi tiềm ẩn đồng thời xảy ra với phản ứng cảm xúc tích cực gia tăng và hưng nhẹ ở những người trưởng thành mới nổi."} +{"idx": 1880091, "en": "Recovery of nutrients from biomass combustion ash is of great importance for sustainable bioenergy waste use. In this work, granulated fertilizer materials were engineered from biofuel bottom ash, lime kiln dust and water, analysed for their chemical complexity and tested in pot experiments (2017-2018) for their propensity to release nutrients. The results obtained in this work showed that spring barley yield was observed to be the highest for granulated biomass ash with 30% of ash in the granule. The yield increased 3.99 t ha-1 per 100 kg ha-1 potassium oxide (K2O) in 2017 and 1.23 t ha-1 per 100 kg ha-1 K2O in 2018. Straw yield varied between 1.39-5.08 t ha-1/100 kg ha-1 in 2017 and 0.36-1.23 t ha-1/100 kg ha-1 in 2018. Calcium concentration significantly increased in soil. No significant changes in soil mobile phosphorus (P) were obtained as well as for the heavy metal concentrations in soil. This suggests that biofuel ash can be a significant source of certain major nutrients for crops that can also beneficially affect soil pH. The results of this work can provide policy-makers with the information needed to diversify existing and enable new biomass bottom ash utilization routes which currently vary significantly between the countries.", "gemini": "Việc thu hồi các chất dinh dưỡng từ tro đốt sinh khối có tầm quan trọng rất lớn đối với việc sử dụng chất thải năng lượng sinh học bền vững. Trong công trình này, các vật liệu phân bón dạng hạt được chế tạo từ tro đáy nhiên liệu sinh học, bụi lò nung vôi và nước, được phân tích về độ phức tạp về mặt hóa học của chúng và được thử nghiệm trong các thí nghiệm chậu (2017-2018) về khả năng giải phóng chất dinh dưỡng của chúng. Kết quả thu được trong công trình này cho thấy năng suất lúa mạch xuân được quan sát là cao nhất đối với tro sinh khối dạng hạt với 30% tro trong hạt. Năng suất tăng 3,99 tấn ha-1 trên 100 kg ha-1 kali oxit (K2O) vào năm 2017 và 1,23 tấn ha-1 trên 100 kg ha-1 K2O vào năm 2018. Năng suất rơm dao động trong khoảng 1,39-5,08 tấn ha-1/100 kg ha-1 vào năm 2017 và 0,36-1,23 tấn ha-1/100 kg ha-1 vào năm 2018. Nồng độ canxi tăng đáng kể trong đất. Không có thay đổi đáng kể nào về phốt pho (P) di động trong đất cũng như nồng độ kim loại nặng trong đất. Điều này cho thấy rằng tro nhiên liệu sinh học có thể là một nguồn cung cấp đáng kể một số chất dinh dưỡng chính cho cây trồng, đồng thời cũng có thể ảnh hưởng có lợi đến độ pH của đất. Kết quả của công trình này có thể cung cấp cho các nhà hoạch định chính sách thông tin cần thiết để đa dạng hóa các tuyến đường sử dụng tro đáy sinh khối hiện có và cho phép các tuyến đường sử dụng mới, hiện đang có sự khác biệt đáng kể giữa các quốc gia."} +{"idx": 1880092, "en": "Aortic interventions in patients with underlying connective tissues disorders present a unique challenge. The inevitable degeneration of the native aorta can lead to the need for multiple staged interventions with significant risk of complications associated with reoperative aortic procedures. We present a challenging case of progressive aortic degeneration in a patient with Marfan syndrome treated with multi-staged open surgical and endovascular procedures.", "gemini": "Các can thiệp động mạch chủ ở những bệnh nhân mắc các rối loạn mô liên kết tiềm ẩn là một thách thức đặc biệt. Sự thoái hóa không thể tránh khỏi của động mạch chủ tự nhiên có thể dẫn đến nhu cầu can thiệp nhiều giai đoạn với nguy cơ biến chứng đáng kể liên quan đến các thủ thuật động mạch chủ tái phẫu thuật. Chúng tôi trình bày một trường hợp khó khăn về thoái hóa động mạch chủ tiến triển ở một bệnh nhân mắc hội chứng Marfan được điều trị bằng các thủ thuật phẫu thuật mở và nội mạch nhiều giai đoạn."} +{"idx": 1880093, "en": "The technology of coal power plant coupled with waste incineration is considered as a promising technology for fossil fuel conservation and waste disposal. In this paper, a system of coal power plant coupled with waste incineration is simulated by Aspen Plus software, and a conventional coal power plant is also simulated for comparison. Comprehensive evaluation including thermodynamic, economic and environmental impact performances are analysed and compared. Evaluation results indicate that the thermodynamic performance and environmental impact of the system of coal power plant coupled with waste incineration are worse, but the economic performance of the system is obviously better than the coal power plant. When the replacement ratio of waste is 20%, the energy and exergy efficiencies of the system are 38.54% and 37.27%, the internal rate of return and discounted payback period of the system are 21.83% and 9.14 years, and the environmental cost of the system is $3597.73 h-1. Therefore, the technology of coal power plant coupled with waste incineration has technical feasibility and economic advantages, and the environmental impacts need to be considered in the application of the technology.", "gemini": "Công nghệ nhà máy nhiệt điện than kết hợp đốt rác được coi là một công nghệ đầy hứa hẹn cho việc bảo tồn nhiên liệu hóa thạch và xử lý rác thải. Trong bài báo này, một hệ thống nhà máy nhiệt điện than kết hợp đốt rác được mô phỏng bằng phần mềm Aspen Plus và một nhà máy nhiệt điện than thông thường cũng được mô phỏng để so sánh. Đánh giá toàn diện bao gồm hiệu suất nhiệt động, kinh tế và tác động môi trường được phân tích và so sánh. Kết quả đánh giá chỉ ra rằng hiệu suất nhiệt động và tác động môi trường của hệ thống nhà máy nhiệt điện than kết hợp đốt rác kém hơn, nhưng hiệu suất kinh tế của hệ thống rõ ràng tốt hơn so với nhà máy nhiệt điện than. Khi tỷ lệ thay thế chất thải là 20%, hiệu suất năng lượng và hiệu suất công suất của hệ thống lần lượt là 38,54% và 37,27%, tỷ suất hoàn vốn nội bộ và thời gian hoàn vốn chiết khấu của hệ thống lần lượt là 21,83% và 9,14 năm, và chi phí môi trường của hệ thống là 3597,73 đô la h-1. Do đó, công nghệ nhà máy nhiệt điện than kết hợp đốt rác có tính khả thi về mặt kỹ thuật và lợi thế về mặt kinh tế, và các tác động môi trường cần được xem xét trong quá trình ứng dụng công nghệ."} +{"idx": 1880094, "en": "In recent years, China has started to develop electric vehicles (EVs) and has become the largest EV market in the world since 2015. Accordingly, the lithium-ion battery (LiB) industry has also been developing quickly in China. However, the Chinese government has decided to cancel the subsidy policy on EVs, which makes the EV market in China unpredictable in the future. Moreover, there will be a considerable number of end-of-life (EoL) EVs and waste LiBs generated in China. These wastes should be appropriately recycled to avoid resource waste or pollution problems. Nevertheless, the quantity and type of EoL EVs and waste LiBs has not been obtained. This research aims at unravelling the trend of EV sales and the volume and type of EoL EVs and waste LiBs in China. We found that it is fair to predict that EVs will increase as the Chinese government has planned even without the subsidy policy. Moreover, we estimated the number of EoL EVs and waste LiBs number based on their calendar lifespan and found that there will be 1.36 million EoL EVs and 11.36 million waste LiBs generated in China in 2030. Furthermore, most of these waste LiBs will be of the nickel cobalt manganese oxide type of ternary LiBs. However, due to the flow of second-hand vehicles from economically developed cities to less economically developed cities, only 400,000 EoL EVs and 3.4 million waste LiBs will be recycled through the formal recycling route. Such information is necessary when evaluating the environmental effect or profitability of the EoL EV and waste LiB recycling industry.", "gemini": "Trong những năm gần đây, Trung Quốc đã bắt đầu phát triển xe điện (EV) và trở thành thị trường EV lớn nhất thế giới kể từ năm 2015. Theo đó, ngành công nghiệp pin lithium-ion (LiB) cũng đã phát triển nhanh chóng tại Trung Quốc. Tuy nhiên, chính phủ Trung Quốc đã quyết định hủy bỏ chính sách trợ cấp đối với xe điện, khiến thị trường xe điện tại Trung Quốc trở nên khó đoán định trong tương lai. Hơn nữa, sẽ có một số lượng đáng kể các loại xe điện hết hạn sử dụng (EoL) và pin LiB thải bỏ được tạo ra tại Trung Quốc. Những chất thải này phải được tái chế thích hợp để tránh lãng phí tài nguyên hoặc các vấn đề ô nhiễm. Tuy nhiên, số lượng và loại xe điện EoL và pin LiB thải bỏ vẫn chưa được biết. Nghiên cứu này nhằm mục đích làm sáng tỏ xu hướng bán xe điện và khối lượng và loại xe điện EoL và pin LiB thải bỏ ở Trung Quốc. Chúng tôi thấy rằng có thể dự đoán rằng xe điện sẽ tăng như chính phủ Trung Quốc đã lên kế hoạch ngay cả khi không có chính sách trợ cấp. Hơn nữa, chúng tôi ước tính số lượng xe điện EoL và số lượng pin LiB thải bỏ dựa trên tuổi thọ theo lịch của chúng và thấy rằng sẽ có 1,36 triệu xe điện EoL và 11,36 triệu pin LiB thải bỏ được tạo ra tại Trung Quốc vào năm 2030. Hơn nữa, hầu hết các loại pin LiB thải bỏ này sẽ là loại ternary LiB có oxit niken coban mangan. Tuy nhiên, do dòng xe cũ từ các thành phố phát triển kinh tế đến các thành phố kém phát triển kinh tế, chỉ có 400.000 xe điện EoL và 3,4 triệu pin LiB thải bỏ sẽ được tái chế thông qua tuyến tái chế chính thức. Thông tin như vậy là cần thiết khi đánh giá tác động môi trường hoặc lợi nhuận của ngành tái chế pin LiB và xe điện EoL."} +{"idx": 1880095, "en": "Prevalence of hepatitis B virus (HBV) infections varies markedly with geography and is endemic in the Arctic. Travel and migration have increased markedly while the influence of migration to high endemic areas remains unknown. We surveyed subjects migrating from an area with a low prevalence of chronic HBV infection (Denmark, 0.01%) to an endemic HBV area (West- and East Greenland, 3% and 29%) in order to describe the prevalence of HBV exposure among migrants. We included 198 Caucasian Danes that had migrated to Greenland and repeated the cross-sectional investigation after 10 years. We performed thorough serological testing for HBV. None had ongoing HBV infection. Migrants to East Greenland were more frequently exposed to HBV than those in West Greenland (34.3% vs 10.3%; p < 0.01). This difference was reduced at 10-year follow-up (8.1% vs 5.7%; ns) and the overall number of participants with past HBV infection decreased over the 10-year period from 19.4% to 6.9% (p = 0.02). In conclusion, migration from very low prevalence to endemic HBV areas associated with a markedly increased risk of exposure to HBV. Lack of vaccination among migrants from Denmark to Greenland was frequent and it poses a continuing risk. All who migrate from low to high endemic HBV areas should be vaccinated. ABBREVIATIONS HBV: Hepatitis B virus; HBV-DNA: Hepatitis B virus deoxyribonucleic acid; HBsAg: Hepatitis B surface antigen; Anti-HBs: Antibodies against hepatitis B surface antigen; Anti-HBc: Antibodies against hepatitis B core antigen; BMI: Body mass index.", "gemini": "Tỷ lệ nhiễm vi-rút viêm gan B (HBV) thay đổi đáng kể theo địa lý và là bệnh đặc hữu ở Bắc Cực. Du lịch và di cư đã tăng lên đáng kể trong khi ảnh hưởng của di cư đến các khu vực đặc hữu cao vẫn chưa được biết. Chúng tôi đã khảo sát những đối tượng di cư từ một khu vực có tỷ lệ nhiễm HBV mãn tính thấp (Đan Mạch, 0,01%) đến một khu vực đặc hữu HBV (Tây và Đông Greenland, 3% và 29%) để mô tả tỷ lệ phơi nhiễm HBV ở những người di cư. Chúng tôi đã đưa vào 198 người Đan Mạch da trắng đã di cư đến Greenland và lặp lại cuộc điều tra cắt ngang sau 10 năm. Chúng tôi đã thực hiện xét nghiệm huyết thanh học kỹ lưỡng đối với HBV. Không ai bị nhiễm HBV đang diễn ra. Những người di cư đến Đông Greenland thường xuyên tiếp xúc với HBV hơn những người ở Tây Greenland (34,3% so với 10,3%; p < 0,01). Sự khác biệt này đã giảm sau 10 năm theo dõi (8,1% so với 5,7%; ns) và tổng số người tham gia bị nhiễm HBV trong quá khứ đã giảm trong giai đoạn 10 năm từ 19,4% xuống 6,9% (p = 0,02). Tóm lại, di cư từ khu vực có tỷ lệ lưu hành rất thấp đến các khu vực đặc hữu HBV liên quan đến nguy cơ phơi nhiễm HBV tăng đáng kể. Việc thiếu vắc-xin ở những người di cư từ Đan Mạch đến Greenland là phổ biến và gây ra nguy cơ liên tục. Tất cả những người di cư từ khu vực có tỷ lệ HBV thấp đến cao đều nên được tiêm vắc-xin. VIẾT TẮT HBV: Vi-rút viêm gan B; HBV-DNA: Axit deoxyribonucleic của vi-rút viêm gan B; HBsAg: Kháng nguyên bề mặt vi-rút viêm gan B; Anti-HBs: Kháng thể chống lại kháng nguyên bề mặt vi-rút viêm gan B; Anti-HBc: Kháng thể chống lại kháng nguyên lõi vi-rút viêm gan B; BMI: Chỉ số khối cơ thể."} +{"idx": 1880096, "en": "Objective Whether Kinesio taping® (KT) might change the endurance of the cervical deep flexor muscles.Methods: Healthy university students were assessed at baseline, immediately after the KT application over the posterior cervical muscles, and at a 24-h follow-up. The clinical assessment of the endurance of the deep cervical flexor muscles using the craniocervical flexion test (CCFT) was performed.Results: Sixty-two students (32 women) with a mean age of 20.4 ± 1.3 years completed the study protocol. Statistically significant pre- to post-taping differences in performance of the craniocervical flexion movement were found between baseline-immediate and baseline-24-h follow-up (p˂0.001). A statistically significant difference in the endurance of the cervical deepflexor muscles was found between baseline-immediate (for stage 2 result and cumulative endurance score), baseline-24-h follow-up (for stage 2result and cumulative endurance score), and immediate-24-h follow-up (for stage 2 result, for cumulative endurance score) p˂0.01.Conclusion: Cervical KT increased endurance for the short term.", "gemini": "Mục tiêu Liệu Kinesio taping® (KT) có thể làm thay đổi sức bền của các cơ gấp sâu cổ hay không. Phương pháp: Các sinh viên đại học khỏe mạnh được đánh giá tại thời điểm ban đầu, ngay sau khi áp dụng KT trên các cơ cổ sau và tại thời điểm theo dõi sau 24 giờ. Đánh giá lâm sàng về sức bền của các cơ gấp sâu cổ bằng cách sử dụng bài kiểm tra gập đầu cổ (CCFT). Kết quả: Sáu mươi hai sinh viên (32 nữ) có độ tuổi trung bình là 20,4 ± 1,3 đã hoàn thành giao thức nghiên cứu. Sự khác biệt có ý nghĩa thống kê trước và sau khi dán băng trong việc thực hiện động tác gập đầu cổ được tìm thấy giữa thời điểm ban đầu-ngay lập tức và thời điểm ban đầu-theo dõi sau 24 giờ (p˂0,001). Sự khác biệt có ý nghĩa thống kê về sức bền của các cơ gấp sâu cổ được tìm thấy giữa thời điểm ban đầu-ngay lập tức (đối với kết quả giai đoạn 2 và điểm sức bền tích lũy), thời điểm ban đầu-theo dõi sau 24 giờ (đối với kết quả giai đoạn 2 và điểm sức bền tích lũy) và thời điểm ngay lập tức-theo dõi sau 24 giờ (đối với kết quả giai đoạn 2, đối với điểm sức bền tích lũy) p˂0,01. Kết luận: KT cổ làm tăng sức bền trong thời gian ngắn."} +{"idx": 1880097, "en": "Falls are the leading cause of injury related death in older adults. In this piece, a motor learning lens is applied to falls, and falls are viewed as three interdependent phases: 1) destabilization, 2) descent, and 3) impact. This review examines how movements can be performed in the descent and impact phases to potentially reduce fall-related injuries. The evidence that movements performed during the descent and impact phases are voluntary motor skills that can be learned by older adults is reviewed. Data from young adult and older adult studies suggest that safe landing strategies can reduce impact force, are voluntary, and are learnable. In conclusion, safe landing strategies may provide a complimentary approach to reduce fall-related injuries.", "gemini": "Ngã là nguyên nhân hàng đầu gây tử vong do chấn thương ở người lớn tuổi. Trong bài viết này, một góc nhìn về quá trình học vận động được áp dụng cho các trường hợp ngã, và ngã được xem là ba giai đoạn phụ thuộc lẫn nhau: 1) mất ổn định, 2) rơi xuống và 3) va chạm. Bài đánh giá này xem xét cách các chuyển động có thể được thực hiện trong các giai đoạn rơi xuống và va chạm để có khả năng làm giảm các chấn thương liên quan đến ngã. Bằng chứng cho thấy các chuyển động được thực hiện trong các giai đoạn rơi xuống và va chạm là các kỹ năng vận động tự nguyện có thể được người lớn tuổi học được. Dữ liệu từ các nghiên cứu trên người lớn tuổi và người trẻ tuổi cho thấy rằng các chiến lược tiếp đất an toàn có thể làm giảm lực va chạm, là tự nguyện và có thể học được. Tóm lại, các chiến lược tiếp đất an toàn có thể cung cấp một cách tiếp cận bổ sung để làm giảm các chấn thương liên quan đến ngã."} +{"idx": 1880098, "en": "We used force matching and verbal reports of finger force to explore a prediction of the iso-perceptual manifold concept, which assumes that stable percepts are associated with a manifold in the afferent-efferent space. Young subjects produced various force magnitudes with the index finger, middle finger, or both fingers together. Further, they reported the force level using a verbal scale and by matching the force with fingers of the contralateral hand. Force matching, but not verbal reports, showed larger variable errors for individual fingers in the two-finger task compared to the single-finger tasks. We discuss possible differences in afferent and efferent contributions to force perception at low and high forces based on the idea of motor control with referent coordinates for the effectors. The differences between the force matching and verbal reports are possibly related to neural circuitry differences between perceiving without action and perceiving-to-act.", "gemini": "Chúng tôi đã sử dụng việc khớp lực và báo cáo bằng lời nói về lực ngón tay để khám phá một dự đoán về khái niệm đa tạp đồng nhận thức, giả định rằng các nhận thức ổn định có liên quan đến một đa tạp trong không gian hướng tâm-hướng ly tâm. Các đối tượng trẻ tuổi tạo ra các độ lớn lực khác nhau bằng ngón trỏ, ngón giữa hoặc cả hai ngón tay cùng nhau. Hơn nữa, họ báo cáo mức độ lực bằng cách sử dụng thang đo bằng lời nói và bằng cách khớp lực với các ngón tay của bàn tay đối diện. Việc khớp lực, nhưng không phải báo cáo bằng lời nói, cho thấy các lỗi biến lớn hơn đối với từng ngón tay trong nhiệm vụ hai ngón tay so với các nhiệm vụ một ngón tay. Chúng tôi thảo luận về những khác biệt có thể có trong các đóng góp hướng tâm và hướng ly tâm đối với nhận thức lực ở lực thấp và lực cao dựa trên ý tưởng về điều khiển vận động với các tọa độ tham chiếu cho các cơ quan hiệu ứng. Sự khác biệt giữa khớp lực và báo cáo bằng lời nói có thể liên quan đến sự khác biệt về mạch thần kinh giữa nhận thức mà không có hành động và nhận thức để hành động."} +{"idx": 1880099, "en": "BACKGROUND AND PURPOSE Previous data suggest patient demographics and clinical presentation are primary predictors of motor recovery poststroke, with minimal contributions of physical interventions. Other studies indicate consistent associations between the amount and intensity of stepping practice with locomotor outcomes. The goal of this study was to determine the relative contributions of these combined variables to locomotor outcomes poststroke across a range of patient demographics and baseline function. METHODS Data were pooled from 3 separate trials evaluating the efficacy of high-intensity training, low-intensity training, and conventional interventions. Demographics, clinical characteristics, and training activities from 144 participants >1-month poststroke were included in stepwise regression analyses to determine their relative contributions to locomotor outcomes. Subsequent latent profile analyses evaluated differences in classes of participants based on their responses to interventions. RESULTS Stepwise regressions indicate primary contributions of stepping activity on locomotor outcomes, with additional influences of age, duration poststroke, and baseline function. Latent profile analyses revealed 2 main classes of outcomes, with the largest gains in those who received high-intensity training and achieved the greatest amounts of stepping practice. Regression and latent profile analyses of only high-intensity training participants indicated age, baseline function, and training activities were primary determinants of locomotor gains. Participants with the smallest gains were older (≈60 years), presented with slower gait speeds (<0.40 m/s), and performed 600 to 1000 less steps/session. CONCLUSIONS Regression and cluster analyses reveal primary contributions of training interventions on mobility outcomes in patients >1-month poststroke. Age, duration poststroke, and baseline impairments were secondary predictors. Registration: URL: https://www.clinicaltrials.gov. Unique identifier: NCT02507466 and NCT01789853.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Các dữ liệu trước đây cho thấy thông tin nhân khẩu học của bệnh nhân và biểu hiện lâm sàng là những yếu tố dự báo chính về khả năng phục hồi vận động sau đột quỵ, với sự đóng góp tối thiểu của các can thiệp vật lý. Các nghiên cứu khác chỉ ra mối liên quan nhất quán giữa lượng và cường độ thực hành bước đi với kết quả vận động. Mục tiêu của nghiên cứu này là xác định sự đóng góp tương đối của các biến kết hợp này vào kết quả vận động sau đột quỵ trên nhiều thông tin nhân khẩu học của bệnh nhân và chức năng cơ bản. PHƯƠNG PHÁP Dữ liệu được tập hợp từ 3 thử nghiệm riêng biệt đánh giá hiệu quả của đào tạo cường độ cao, đào tạo cường độ thấp và các can thiệp thông thường. Thông tin nhân khẩu học, đặc điểm lâm sàng và hoạt động đào tạo từ 144 người tham gia > 1 tháng sau đột quỵ đã được đưa vào phân tích hồi quy từng bước để xác định sự đóng góp tương đối của họ vào kết quả vận động. Các phân tích cấu hình tiềm ẩn tiếp theo đã đánh giá sự khác biệt về các lớp người tham gia dựa trên phản ứng của họ đối với các can thiệp. KẾT QUẢ Các hồi quy từng bước chỉ ra sự đóng góp chính của hoạt động bước đi vào kết quả vận động, với các ảnh hưởng bổ sung của tuổi, thời gian sau đột quỵ và chức năng cơ bản. Các phân tích cấu hình tiềm ẩn cho thấy 2 lớp kết quả chính, với mức tăng lớn nhất ở những người được đào tạo cường độ cao và đạt được số lần thực hành bước đi nhiều nhất. Các phân tích hồi quy và cấu hình tiềm ẩn chỉ đối với những người tham gia đào tạo cường độ cao cho thấy tuổi, chức năng cơ bản và hoạt động đào tạo là những yếu tố quyết định chính của mức tăng vận động. Những người tham gia có mức tăng nhỏ nhất thường lớn tuổi hơn (≈60 tuổi), có tốc độ đi chậm hơn (<0,40 m/giây) và thực hiện ít hơn 600 đến 1000 bước/buổi. KẾT LUẬN Các phân tích hồi quy và cụm cho thấy sự đóng góp chính của các can thiệp đào tạo vào kết quả vận động ở những bệnh nhân > 1 tháng sau đột quỵ. Tuổi, thời gian sau đột quỵ và suy giảm chức năng cơ bản là những yếu tố dự báo thứ cấp. Đăng ký: URL: https://www.clinicaltrials.gov. Mã định danh duy nhất: NCT02507466 và NCT01789853."} +{"idx": 1880100, "en": "BACKGROUND AND PURPOSE Understanding the mechanisms underlying progression/regression of symptomatic intracranial atherosclerotic stenosis (sICAS) will inform secondary prevention of the patients. Focal wall shear stress (WSS) may play an important role, which, however, had seldom been investigated. METHODS Patients with acute ischemic stroke or transient ischemic attack (TIA) attributed to 50% to 99% intracranial atherosclerotic stenosis were recruited. All patients underwent cerebral computed tomography angiography at baseline, and a computational fluid dynamics model was built based on computed tomography angiography to simulate blood flow and quantify WSS in the vicinity of the sICAS lesion. All patients received optimal medical treatment and a second computed tomography angiography at 1 year. The change in the luminal stenosis from baseline to 1 year in sICAS was defined as progression (increased >10%), quiescence (±10%), or regression (decreased >10%). Associations between baseline WSS metrics and sICAS regression were analyzed. RESULTS Among 39 patients (median age 62 years; 27 males), sICAS luminal stenosis progressed, remained quiescent and regressed in 6 (15.4%), 15 (38.5%), and 18 (46.2%) cases, respectively. A higher maximum WSS and larger high-WSS area, throughout the sICAS lesion or obtained separately in the proximal and distal parts of the lesion, were independently associated with regression of luminal stenosis in sICAS over 1 year. CONCLUSIONS A majority of sICAS lesions regress or stay quiescent in the luminal stenosis over 1 year after stroke under optimal medical treatment, when higher focal WSS may facilitate stenosis regression. Further studies of the effects of hemodynamics including WSS in altering plaque vulnerability and stroke risks are needed.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Hiểu được các cơ chế cơ bản của sự tiến triển/thoái triển của hẹp động mạch não có triệu chứng (sICAS) sẽ cung cấp thông tin về biện pháp phòng ngừa thứ cấp cho bệnh nhân. Độ cắt ứng suất thành (WSS) cục bộ có thể đóng một vai trò quan trọng, tuy nhiên, điều này hiếm khi được nghiên cứu. PHƯƠNG PHÁP Những bệnh nhân bị đột quỵ thiếu máu cục bộ cấp hoặc cơn thiếu máu não thoáng qua (TIA) do hẹp động mạch não từ 50% đến 99% đã được tuyển dụng. Tất cả bệnh nhân đều được chụp động mạch não cắt lớp vi tính ở thời điểm ban đầu và một mô hình động lực học chất lỏng tính toán đã được xây dựng dựa trên chụp động mạch não cắt lớp vi tính để mô phỏng dòng máu và định lượng WSS trong vùng lân cận của tổn thương sICAS. Tất cả bệnh nhân đều được điều trị nội khoa tối ưu và chụp động mạch não cắt lớp vi tính lần thứ hai sau 1 năm. Sự thay đổi về hẹp lòng mạch từ ban đầu đến 1 năm ở sICAS được định nghĩa là tiến triển (tăng > 10%), ổn định (± 10%) hoặc thoái triển (giảm > 10%). Các mối liên quan giữa các số liệu WSS ban đầu và sự thoái triển của sICAS đã được phân tích. KẾT QUẢ Trong số 39 bệnh nhân (tuổi trung bình 62; 27 nam), hẹp lòng mạch sICAS tiến triển, ổn định và thoái triển ở lần lượt 6 (15,4%), 15 (38,5%) và 18 (46,2%) trường hợp. WSS tối đa cao hơn và diện tích WSS cao lớn hơn, trên toàn bộ tổn thương sICAS hoặc thu được riêng biệt ở các phần gần và xa của tổn thương, có liên quan độc lập với sự thoái triển của hẹp lòng mạch ở sICAS trong hơn 1 năm. KẾT LUẬN Phần lớn các tổn thương sICAS thoái triển hoặc ổn định ở hẹp lòng mạch trong hơn 1 năm sau đột quỵ khi được điều trị nội khoa tối ưu, khi WSS cục bộ cao hơn có thể thúc đẩy sự thoái triển hẹp. Cần có thêm các nghiên cứu về tác động của huyết động học bao gồm WSS trong việc thay đổi tình trạng dễ bị tổn thương của mảng bám và nguy cơ đột quỵ."} +{"idx": 1880101, "en": "BACKGROUND A large number of experimental studies show that the mutation and regulation of long non-coding RNAs (lncRNAs) are associated with various human diseases. Accurate prediction of lncRNA-disease associations can provide a new perspective for the diagnosis and treatment of diseases. The main function of many lncRNAs is still unclear and using traditional experiments to detect lncRNA-disease associations is time-consuming. RESULTS In this paper, we develop a novel and effective method for the prediction of lncRNA-disease associations using network feature similarity and gradient boosting (LDNFSGB). In LDNFSGB, we first construct a comprehensive feature vector to effectively extract the global and local information of lncRNAs and diseases through considering the disease semantic similarity (DISSS), the lncRNA function similarity (LNCFS), the lncRNA Gaussian interaction profile kernel similarity (LNCGS), the disease Gaussian interaction profile kernel similarity (DISGS), and the lncRNA-disease interaction (LNCDIS). Particularly, two methods are used to calculate the DISSS (LNCFS) for considering the local and global information of disease semantics (lncRNA functions) respectively. An autoencoder is then used to reduce the dimensionality of the feature vector to obtain the optimal feature parameter from the original feature set. Furthermore, we employ the gradient boosting algorithm to obtain the lncRNA-disease association prediction. CONCLUSIONS In this study, hold-out, leave-one-out cross-validation, and ten-fold cross-validation methods are implemented on three publicly available datasets to evaluate the performance of LDNFSGB. Extensive experiments show that LDNFSGB dramatically outperforms other state-of-the-art methods. The case studies on six diseases, including cancers and non-cancers, further demonstrate the effectiveness of our method in real-world applications.", "gemini": "BỐI CẢNH Một số lượng lớn các nghiên cứu thực nghiệm cho thấy đột biến và điều hòa của RNA không mã hóa dài (lncRNA) có liên quan đến nhiều bệnh ở người. Việc dự đoán chính xác các mối liên quan giữa bệnh lncRNA có thể cung cấp một góc nhìn mới cho việc chẩn đoán và điều trị các bệnh. Chức năng chính của nhiều lncRNA vẫn chưa rõ ràng và việc sử dụng các thí nghiệm truyền thống để phát hiện các mối liên quan giữa bệnh lncRNA rất tốn thời gian. KẾT QUẢ Trong bài báo này, chúng tôi phát triển một phương pháp mới và hiệu quả để dự đoán các mối liên quan giữa bệnh lncRNA bằng cách sử dụng sự tương đồng tính năng mạng và tăng cường gradient (LDNFSGB). Trong LDNFSGB, trước tiên chúng tôi xây dựng một vectơ đặc trưng toàn diện để trích xuất hiệu quả thông tin cục bộ và toàn cục của lncRNA và các bệnh thông qua việc xem xét sự tương đồng ngữ nghĩa của bệnh (DISSS), sự tương đồng chức năng lncRNA (LNCFS), sự tương đồng hạt nhân tương tác Gaussian của lncRNA (LNCGS), sự tương đồng hạt nhân tương tác Gaussian của bệnh (DISGS) và tương tác lncRNA-bệnh (LNCDIS). Đặc biệt, hai phương pháp được sử dụng để tính toán DISSS (LNCFS) để xem xét thông tin cục bộ và toàn cục về ngữ nghĩa của bệnh (chức năng lncRNA) tương ứng. Sau đó, một bộ mã hóa tự động được sử dụng để giảm chiều của vectơ đặc trưng để có được tham số đặc trưng tối ưu từ tập hợp đặc trưng ban đầu. Hơn nữa, chúng tôi sử dụng thuật toán tăng cường gradient để có được dự đoán mối liên quan giữa bệnh lncRNA. KẾT LUẬN Trong nghiên cứu này, các phương pháp kiểm tra chéo loại trừ, kiểm tra chéo bỏ một lần và kiểm tra chéo mười lần được thực hiện trên ba bộ dữ liệu có sẵn công khai để đánh giá hiệu suất của LDNFSGB. Các thí nghiệm mở rộng cho thấy LDNFSGB vượt trội đáng kể so với các phương pháp tiên tiến khác. Các nghiên cứu trường hợp trên sáu bệnh, bao gồm ung thư và không ung thư, càng chứng minh thêm hiệu quả của phương pháp của chúng tôi trong các ứng dụng thực tế."} +{"idx": 1880102, "en": "BACKGROUND Based on the previous investigation in our institution, the incidence of intraoperative hypothermia in neonates was high. Since September 1st, 2019, the recommendation had been launched to utilize ≤1 L/min fresh gas flow during the neonates' surgical procedure. We therefore intended to evaluate the association between low fresh gas flow anesthesia and the occurrence of hypothermia in neonates undergoing digestive surgeries. METHODS A retrospective chart review, before-after study was conducted for neonates who underwent digestive surgeries. The primary outcomes were the incidence of hypothermia. The secondary outcomes included hospital mortality, the value of lowest temperature, blood loss, mean body temperature during the surgery, the length of hypothermia during the surgery and postoperative hospital length-of- stay (PLOS). RESULTS 249 neonates fulfilled the eligibility criteria. The overall incidence of intraoperative hypothermia was 81.9%. The low fresh gas flow anesthesia significantly reduced the odds of hypothermia [routine group: 149 (87.6%) versus low flow group: 55 (69.6%); p < 0.01]. Moreover, the low fresh gas flow anesthesia could reduce the length of hypothermia [routine group: 104 mins (50, 156) versus low flow group: 30 mins (0,100); p < 0.01], as well as elevate the value of lowest temperature for neonates [routine group: 35.1 °C (34.5, 35.7) versus low flow group: 35.7 °C (35.3, 36); p < 0.01]. After adjustment for confounding, low fresh gas flow anesthesia and the length of surgical time were independently associated with intraoperative hypothermia. CONCLUSIONS Low fresh gas flow anesthesia is an effective way to alleviate hypothermia in neonates undergoing open digestive surgery.", "gemini": "BỐI CẢNH Dựa trên các nghiên cứu trước đây tại cơ sở của chúng tôi, tỷ lệ hạ thân nhiệt trong mổ ở trẻ sơ sinh là cao. Kể từ ngày 1 tháng 9 năm 2019, khuyến cáo đã được đưa ra để sử dụng lưu lượng khí tươi ≤1 lít/phút trong quá trình phẫu thuật cho trẻ sơ sinh. Do đó, chúng tôi dự định đánh giá mối liên quan giữa gây mê lưu lượng khí tươi thấp và tình trạng hạ thân nhiệt ở trẻ sơ sinh phải phẫu thuật tiêu hóa. PHƯƠNG PHÁP Một nghiên cứu đánh giá biểu đồ hồi cứu trước sau đã được tiến hành đối với trẻ sơ sinh phải phẫu thuật tiêu hóa. Kết quả chính là tỷ lệ hạ thân nhiệt. Các kết quả phụ bao gồm tỷ lệ tử vong tại bệnh viện, giá trị nhiệt độ thấp nhất, mất máu, nhiệt độ cơ thể trung bình trong quá trình phẫu thuật, thời gian hạ thân nhiệt trong quá trình phẫu thuật và thời gian nằm viện sau phẫu thuật (PLOS). KẾT QUẢ 249 trẻ sơ sinh đáp ứng đủ tiêu chuẩn. Tỷ lệ hạ thân nhiệt trong mổ là 81,9%. Gây mê lưu lượng khí tươi thấp làm giảm đáng kể tỷ lệ hạ thân nhiệt [nhóm thường quy: 149 (87,6%) so với nhóm lưu lượng thấp: 55 (69,6%); p < 0,01]. Hơn nữa, gây mê lưu lượng khí tươi thấp có thể làm giảm thời gian hạ thân nhiệt [nhóm thường quy: 104 phút (50, 156) so với nhóm lưu lượng thấp: 30 phút (0,100); p < 0,01], cũng như nâng cao giá trị nhiệt độ thấp nhất ở trẻ sơ sinh [nhóm thường quy: 35,1 °C (34,5, 35,7) so với nhóm lưu lượng thấp: 35,7 °C (35,3, 36); p < 0,01]. Sau khi điều chỉnh các yếu tố gây nhiễu, gây mê lưu lượng khí tươi thấp và thời gian phẫu thuật có liên quan độc lập với hạ thân nhiệt trong mổ. KẾT LUẬN Gây mê lưu lượng khí tươi thấp là một cách hiệu quả để giảm hạ thân nhiệt ở trẻ sơ sinh phải phẫu thuật tiêu hóa mở."} +{"idx": 1880103, "en": "BACKGROUND Immune-response (IR) genes have an important role in the defense against highly variable pathogens, and therefore, diversity in these genomic regions is essential for species' survival and adaptation. Although current genome assemblies from Old World camelids are very useful for investigating genome-wide diversity, demography and population structure, they have inconsistencies and gaps that limit analyses at local genomic scales. Improved and more accurate genome assemblies and annotations are needed to study complex genomic regions like adaptive and innate IR genes. RESULTS In this work, we improved the genome assemblies of the three Old World camel species - domestic dromedary and Bactrian camel, and the two-humped wild camel - via different computational methods. The newly annotated dromedary genome assembly CamDro3 served as reference to scaffold the NCBI RefSeq genomes of domestic Bactrian and wild camels. These upgraded assemblies were then used to assess nucleotide diversity of IR genes within and between species, and to compare the diversity found in immune genes and the rest of the genes in the genome. We detected differences in the nucleotide diversity among the three Old World camelid species and between IR gene groups, i.e., innate versus adaptive. Among the three species, domestic Bactrian camels showed the highest mean nucleotide diversity. Among the functionally different IR gene groups, the highest mean nucleotide diversity was observed in the major histocompatibility complex. CONCLUSIONS The new camel genome assemblies were greatly improved in terms of contiguity and increased size with fewer scaffolds, which is of general value for the scientific community. This allowed us to perform in-depth studies on genetic diversity in immunity-related regions of the genome. Our results suggest that differences of diversity across classes of genes appear compatible with a combined role of population history and differential exposures to pathogens, and consequent different selective pressures.", "gemini": "BỐI CẢNH Các gen đáp ứng miễn dịch (IR) có vai trò quan trọng trong việc chống lại các tác nhân gây bệnh có khả năng thay đổi cao, do đó, sự đa dạng trong các vùng bộ gen này rất cần thiết cho sự sống còn và thích nghi của loài. Mặc dù các bộ gen hiện tại từ lạc đà Cựu thế giới rất hữu ích để nghiên cứu sự đa dạng trên toàn bộ bộ gen, nhân khẩu học và cấu trúc quần thể, nhưng chúng có những điểm không nhất quán và khoảng trống hạn chế khả năng phân tích ở quy mô bộ gen cục bộ. Cần có các bộ gen và chú thích được cải thiện và chính xác hơn để nghiên cứu các vùng bộ gen phức tạp như các gen IR thích nghi và bẩm sinh. KẾT QUẢ Trong công trình này, chúng tôi đã cải thiện bộ gen của ba loài lạc đà Cựu thế giới - lạc đà một bướu trong nước và lạc đà hai bướu, và lạc đà hoang dã hai bướu - thông qua các phương pháp tính toán khác nhau. Bộ gen lạc đà một bướu mới được chú thích CamDro3 đóng vai trò là tài liệu tham khảo để xây dựng giàn giáo cho bộ gen NCBI RefSeq của lạc đà hai bướu trong nước và lạc đà hoang dã. Sau đó, các bộ gen nâng cấp này được sử dụng để đánh giá sự đa dạng của nucleotide của các gen IR trong và giữa các loài, và để so sánh sự đa dạng được tìm thấy trong các gen miễn dịch và các gen còn lại trong bộ gen. Chúng tôi phát hiện ra sự khác biệt về đa dạng nucleotide giữa ba loài lạc đà Cựu thế giới và giữa các nhóm gen IR, tức là bẩm sinh so với thích nghi. Trong số ba loài, lạc đà hai bướu trong nước cho thấy sự đa dạng nucleotide trung bình cao nhất. Trong số các nhóm gen IR có chức năng khác nhau, sự đa dạng nucleotide trung bình cao nhất được quan sát thấy ở phức hợp tương hợp mô chính. KẾT LUẬN Các bộ gen lạc đà mới đã được cải thiện đáng kể về tính liên tục và tăng kích thước với ít giàn giáo hơn, có giá trị chung cho cộng đồng khoa học. Điều này cho phép chúng tôi thực hiện các nghiên cứu chuyên sâu về sự đa dạng di truyền trong các vùng bộ gen liên quan đến miễn dịch. Kết quả của chúng tôi cho thấy sự khác biệt về tính đa dạng giữa các lớp gen có vẻ phù hợp với vai trò kết hợp của tiền sử quần thể và sự tiếp xúc khác nhau với các tác nhân gây bệnh, và do đó có áp lực chọn lọc khác nhau."} +{"idx": 1880104, "en": "BACKGROUND To fully elucidate the roles and mechanisms of plant hormones in leaf senescence, we adopted an integrated analysis of both non-senescing and senescing leaves from red maple with transcriptome and metabolome data. RESULTS Transcription and metabolite profiles were generated through a combination of deep sequencing, third-generation sequencing data analysis, and ultrahigh-performance liquid chromatograph Q extractive mass spectrometry (UHPLC-QE-MS), respectively. We investigated the accumulation of compounds and the expression of biosynthesis and signaling genes for eight hormones. The results revealed that ethylene and abscisic acid concentrations increased during the leaf senescence process, while the contents of cytokinin, auxin, jasmonic acid, and salicylic acid continued to decrease. Correlation tests between the hormone content and transcriptional changes were analyzed, and in six pathways, genes closely linked with leaf senescence were identified. CONCLUSIONS These results will enrich our understanding of the mechanisms of plant hormones that regulate leaf senescence in red maple, while establishing a foundation for the genetic modification of Acer in the future.", "gemini": "BỐI CẢNH Để làm sáng tỏ hoàn toàn vai trò và cơ chế của hormone thực vật trong quá trình lão hóa lá, chúng tôi đã áp dụng phân tích tích hợp cả lá không lão hóa và lá lão hóa từ cây thích đỏ với dữ liệu chuyển录 và chuyển hóa. KẾT QUẢ Hồ sơ chuyển hóa và chuyển录 được tạo ra thông qua sự kết hợp của giải trình tự sâu, phân tích dữ liệu giải trình tự thế hệ thứ ba và sắc ký lỏng hiệu suất cực cao Q phổ khối trích xuất (UHPLC-QE-MS). Chúng tôi đã điều tra sự tích lũy các hợp chất và sự biểu hiện của các gen sinh tổng hợp và truyền tín hiệu cho tám hormone. Kết quả cho thấy nồng độ ethylene và axit abscisic tăng lên trong quá trình lão hóa lá, trong khi hàm lượng cytokinin, auxin, axit jasmonic và axit salicylic tiếp tục giảm. Các xét nghiệm tương quan giữa hàm lượng hormone và các thay đổi phiên mã đã được phân tích và trong sáu con đường, các gen có liên quan chặt chẽ với quá trình lão hóa lá đã được xác định. KẾT LUẬN Những kết quả này sẽ làm phong phú thêm hiểu biết của chúng ta về các cơ chế của hormone thực vật điều chỉnh quá trình lão hóa lá ở cây thích đỏ, đồng thời thiết lập nền tảng cho việc biến đổi gen của Acer trong tương lai."} +{"idx": 1880105, "en": "BACKGROUND C-to-U RNA editing is prevalent in the mitochondrial and chloroplast genes in plants. The biological functions of a fraction of C-to-U editing sites are continuously discovered by case studies. However, at genome-wide level, the cis and trans determinants affecting the occurrence or editing levels of these C-to-U events are relatively less studied. What is known is that the PPR (pentatricopeptide repeat) proteins are the main trans-regulatory elements responsible for the C-to-U conversion, but other determinants especially the cis-regulatory elements remain largely uninvestigated. RESULTS By analyzing the transcriptome and translatome data in Arabidopsis thaliana roots and shoots, combined with RNA-seq data from hybrids of Arabidopsis thaliana and Arabidopsis lyrata, we perform genome-wide investigation on the cis elements and trans-regulatory elements that potentially affect C-to-U editing events. An upstream guanosine or double-stranded RNA (dsRNA) regions are unfavorable for editing events. Meanwhile, many genes including the transcription factors may indirectly play regulatory roles in trans. CONCLUSIONS The 5-prime thymidine facilitates editing and dsRNA structures prevent editing in cis. Many transcription factors affect editing in trans. Although the detailed molecular mechanisms underlying the cis and trans regulation remain to be experimentally verified, our findings provide novel aspects in studying the botanical C-to-U RNA editing events.", "gemini": "HẬU CẢNH Việc chỉnh sửa RNA C thành U rất phổ biến ở các gen ty thể và lục lạp ở thực vật. Các chức năng sinh học của một phần các vị trí chỉnh sửa C thành U liên tục được phát hiện thông qua các nghiên cứu trường hợp. Tuy nhiên, ở cấp độ toàn bộ hệ gen, các yếu tố xác định cis và trans ảnh hưởng đến sự xuất hiện hoặc mức chỉnh sửa của các sự kiện C thành U này vẫn chưa được nghiên cứu nhiều. Những gì đã biết là các protein PPR (lặp lại pentatricopeptide) là các yếu tố điều hòa trans chính chịu trách nhiệm cho quá trình chuyển đổi C thành U, nhưng các yếu tố xác định khác, đặc biệt là các yếu tố điều hòa cis vẫn chưa được nghiên cứu nhiều. KẾT QUẢ Bằng cách phân tích dữ liệu transcriptome và translatome ở rễ và chồi của Arabidopsis thaliana, kết hợp với dữ liệu RNA-seq từ các giống lai của Arabidopsis thaliana và Arabidopsis lyrata, chúng tôi tiến hành nghiên cứu trên toàn bộ hệ gen về các yếu tố cis và các yếu tố điều hòa trans có khả năng ảnh hưởng đến các sự kiện chỉnh sửa C thành U. Một vùng guanosine hoặc vùng RNA mạch đôi (dsRNA) ở thượng nguồn không thuận lợi cho các sự kiện chỉnh sửa. Trong khi đó, nhiều gen bao gồm các yếu tố phiên mã có thể đóng vai trò điều hòa gián tiếp trong trans. KẾT LUẬN Thymidine 5 đầu tạo điều kiện cho việc chỉnh sửa và các cấu trúc dsRNA ngăn chặn việc chỉnh sửa trong cis. Nhiều yếu tố phiên mã ảnh hưởng đến việc chỉnh sửa trong trans. Mặc dù các cơ chế phân tử chi tiết cơ bản cho quá trình điều hòa cis và trans vẫn cần được xác minh bằng thực nghiệm, nhưng những phát hiện của chúng tôi cung cấp các khía cạnh mới trong việc nghiên cứu các sự kiện chỉnh sửa RNA C thành U của thực vật."} +{"idx": 1880106, "en": "BACKGROUND Argeia pugettensis is an isopod species that parasitizes other crustaceans. Its huge native geographic range spans the Pacific from China to California, but molecular data are available only for a handful of specimens from North-American populations. We sequenced and characterised the complete mitogenome of a specimen collected in the Yellow Sea. RESULTS It exhibited a barcode (cox1) similarity level of only 87-89% with North-American populations, which is unusually low for conspecifics. Its mitogenome is among the largest in isopods (≈16.5 Kbp), mostly due to a large duplicated palindromic genomic segment (2 Kbp) comprising three genes. However, it lost a segment comprising three genes, nad4L-trnP-nad6, and many genes exhibited highly divergent sequences in comparison to isopod orthologues, including numerous mutations, deletions and insertions. Phylogenetic and selection analyses corroborated that this is one of the handful of most rapidly evolving available isopod mitogenomes, and that it evolves under highly relaxed selection constraints (as opposed to positive selection). However, its nuclear 18S gene is highly conserved, which suggests that rapid evolution is limited to its mitochondrial genome. The cox1 sequence analysis indicates that elevated mitogenomic evolutionary rates are not shared by North-American conspecifics, which suggests a breakdown of cox1 barcoding in this species. CONCLUSIONS A highly architecturally disrupted mitogenome and decoupling of mitochondrial and nuclear rates would normally be expected to have strong negative impacts on the fitness of the organism, so the existence of this lineage is a puzzling evolutionary question. Additional studies are needed to assess the phylogenetic breadth of this disrupted mitochondrial architecture and its impact on fitness.", "gemini": "HẬU CẢNH Argeia pugettensis là một loài chân đều ký sinh trên các loài giáp xác khác. Phạm vi địa lý bản địa rộng lớn của nó trải dài khắp Thái Bình Dương từ Trung Quốc đến California, nhưng dữ liệu phân tử chỉ có sẵn cho một số ít mẫu vật từ các quần thể Bắc Mỹ. Chúng tôi đã giải trình tự và mô tả toàn bộ mitogenome của một mẫu vật được thu thập ở Biển Vàng. KẾT QUẢ Nó thể hiện mức độ tương đồng của mã vạch (cox1) chỉ 87-89% với các quần thể Bắc Mỹ, mức này thấp bất thường đối với các loài đồng loại. Mitogenome của nó nằm trong số những loài lớn nhất trong số các loài chân đều (≈16,5 Kbp), chủ yếu là do một đoạn gen palindromic trùng lặp lớn (2 Kbp) bao gồm ba gen. Tuy nhiên, nó đã mất một phân đoạn bao gồm ba gen, nad4L-trnP-nad6 và nhiều gen biểu hiện các trình tự rất khác biệt so với các gen đồng nguồn của chân đều, bao gồm nhiều đột biến, mất và chèn. Các phân tích phát sinh loài và chọn lọc đã chứng thực rằng đây là một trong số ít các mitogenome của chân đều có tốc độ tiến hóa nhanh nhất hiện có và nó tiến hóa theo các ràng buộc chọn lọc rất thoải mái (trái ngược với chọn lọc tích cực). Tuy nhiên, gen 18S hạt nhân của nó được bảo tồn cao, điều này cho thấy quá trình tiến hóa nhanh chóng chỉ giới hạn ở bộ gen ty thể của nó. Phân tích trình tự cox1 chỉ ra rằng các loài đồng loại Bắc Mỹ không có chung tỷ lệ tiến hóa mitogenome cao, điều này cho thấy sự phá vỡ mã vạch cox1 ở loài này. KẾT LUẬN Một mitogenome bị phá vỡ về mặt kiến trúc và sự tách biệt giữa tỷ lệ ty thể và hạt nhân thường được dự kiến sẽ có tác động tiêu cực mạnh đến thể lực của sinh vật, vì vậy sự tồn tại của dòng dõi này là một câu hỏi tiến hóa khó hiểu. Cần có thêm các nghiên cứu để đánh giá phạm vi phát sinh loài của kiến trúc ty thể bị phá vỡ này và tác động của nó đến thể lực."} +{"idx": 1880107, "en": "BACKGROUND The study of speciation has expanded with the increasing availability and affordability of high-resolution genomic data. How the genome evolves throughout the process of divergence and which regions of the genome are responsible for causing and maintaining that divergence have been central questions in recent work. Here, we use three pairs of species from the recently diverged bee hummingbird clade to investigate differences in the genome at different stages of speciation, using divergence times as a proxy for the speciation continuum. RESULTS Population measures of relative differentiation between hybridizing species reveal that different chromosome types diverge at different stages of speciation. Using FST as our relative measure of differentiation we found that the sex chromosome shows signs of divergence early in speciation. Next, small autosomes (microchromosomes) accumulate highly diverged genomic regions, while the large autosomes (macrochromosomes) accumulate genomic regions of divergence at a later stage of speciation. CONCLUSIONS Our finding that genomic windows of elevated FST accumulate on small autosomes earlier in speciation than on larger autosomes is counter to the prediction that FST increases with size of chromosome (i.e. with decreased recombination rate), and is not represented when weighted average FST per chromosome is compared with chromosome size. The results of this study suggest that multiple chromosome characteristics such as recombination rate and gene density combine to influence the genomic locations of signatures of divergence.", "gemini": "HẬU CẢNH Nghiên cứu về quá trình hình thành loài đã được mở rộng với sự sẵn có và khả năng chi trả ngày càng tăng của dữ liệu bộ gen có độ phân giải cao. Bộ gen tiến hóa như thế nào trong suốt quá trình phân kỳ và những vùng nào của bộ gen chịu trách nhiệm gây ra và duy trì sự phân kỳ đó là những câu hỏi trọng tâm trong công trình gần đây. Ở đây, chúng tôi sử dụng ba cặp loài từ nhánh chim ruồi ong mới phân kỳ để nghiên cứu sự khác biệt trong bộ gen ở các giai đoạn khác nhau của quá trình hình thành loài, sử dụng thời gian phân kỳ làm đại diện cho sự liên tục của quá trình hình thành loài. KẾT QUẢ Các biện pháp quần thể về sự phân biệt tương đối giữa các loài lai tạo cho thấy các loại nhiễm sắc thể khác nhau phân kỳ ở các giai đoạn khác nhau của quá trình hình thành loài. Sử dụng FST làm biện pháp tương đối về sự phân biệt, chúng tôi thấy rằng nhiễm sắc thể giới tính có dấu hiệu phân kỳ sớm trong quá trình hình thành loài. Tiếp theo, các nhiễm sắc thể thường nhỏ (vi nhiễm sắc thể) tích lũy các vùng bộ gen phân kỳ cao, trong khi các nhiễm sắc thể thường lớn (đại nhiễm sắc thể) tích lũy các vùng bộ gen phân kỳ ở giai đoạn sau của quá trình hình thành loài. KẾT LUẬN Phát hiện của chúng tôi là các cửa sổ bộ gen của FST tăng cao tích lũy trên các nhiễm sắc thể thường nhỏ sớm hơn trong quá trình hình thành loài so với các nhiễm sắc thể thường lớn hơn trái ngược với dự đoán rằng FST tăng theo kích thước nhiễm sắc thể (tức là với tỷ lệ tái tổ hợp giảm) và không được thể hiện khi FST trung bình có trọng số trên mỗi nhiễm sắc thể được so sánh với kích thước nhiễm sắc thể. Kết quả của nghiên cứu này cho thấy nhiều đặc điểm của nhiễm sắc thể như tỷ lệ tái tổ hợp và mật độ gen kết hợp lại để ảnh hưởng đến vị trí bộ gen của các dấu hiệu phân kỳ."} +{"idx": 1880108, "en": "BACKGROUND More than 80,000 dengue cases including 215 deaths were reported nationally in less than 7 months between 2016 and 2017, a fourfold increase in the number of reported cases compared to the average number over 2010-2016. The region of Negombo, located in the Western province, experienced the greatest number of dengue cases in the country and is the focus area of our study, where we aim to capture the spatial-temporal dynamics of dengue transmission. METHODS We present a statistical modeling framework to evaluate the spatial-temporal dynamics of the 2016-2017 dengue outbreak in the Negombo region of Sri Lanka as a function of human mobility, land-use, and climate patterns. The analysis was conducted at a 1 km × 1 km spatial resolution and a weekly temporal resolution. RESULTS Our results indicate human mobility to be a stronger indicator for local outbreak clusters than land-use or climate variables. The minimum daily temperature was identified as the most influential climate variable on dengue cases in the region; while among the set of land-use patterns considered, urban areas were found to be most prone to dengue outbreak, followed by areas with stagnant water and then coastal areas. The results are shown to be robust across spatial resolutions. CONCLUSIONS Our study highlights the potential value of using travel data to target vector control within a region. In addition to illustrating the relative relationship between various potential risk factors for dengue outbreaks, the results of our study can be used to inform where and when new cases of dengue are likely to occur within a region, and thus help more effectively and innovatively, plan for disease surveillance and vector control.", "gemini": "BỐI CẢNH Hơn 80.000 ca sốt xuất huyết bao gồm 215 ca tử vong đã được báo cáo trên toàn quốc trong vòng chưa đầy 7 tháng từ năm 2016 đến 2017, tăng gấp bốn lần số ca được báo cáo so với số ca trung bình trong giai đoạn 2010-2016. Khu vực Negombo, nằm ở tỉnh phía Tây, có số ca mắc sốt xuất huyết nhiều nhất cả nước và là trọng tâm nghiên cứu của chúng tôi, nơi chúng tôi đặt mục tiêu nắm bắt được sự thay đổi về mặt không gian - thời gian của quá trình lây truyền sốt xuất huyết. PHƯƠNG PHÁP Chúng tôi trình bày một khuôn khổ mô hình thống kê để đánh giá sự thay đổi về mặt không gian - thời gian của đợt bùng phát sốt xuất huyết năm 2016-2017 ở khu vực Negombo của Sri Lanka như một hàm của sự di chuyển của con người, sử dụng đất và các kiểu khí hậu. Phân tích được thực hiện ở độ phân giải không gian 1 km × 1 km và độ phân giải thời gian hàng tuần. KẾT QUẢ Kết quả của chúng tôi chỉ ra rằng sự di chuyển của con người là một chỉ báo mạnh hơn đối với các nhóm bùng phát cục bộ so với các biến về sử dụng đất hoặc khí hậu. Nhiệt độ tối thiểu hàng ngày được xác định là biến khí hậu có ảnh hưởng lớn nhất đến các ca mắc sốt xuất huyết trong khu vực; trong khi trong số các kiểu sử dụng đất được xem xét, các khu vực đô thị được phát hiện là dễ bùng phát sốt xuất huyết nhất, tiếp theo là các khu vực có nước đọng và sau đó là các khu vực ven biển. Kết quả cho thấy có độ tin cậy cao trên các độ phân giải không gian. KẾT LUẬN Nghiên cứu của chúng tôi nêu bật giá trị tiềm năng của việc sử dụng dữ liệu đi lại để nhắm mục tiêu kiểm soát véc tơ trong một khu vực. Ngoài việc minh họa mối quan hệ tương đối giữa các yếu tố nguy cơ tiềm ẩn khác nhau đối với các đợt bùng phát sốt xuất huyết, kết quả nghiên cứu của chúng tôi có thể được sử dụng để thông báo về nơi và thời điểm có khả năng xảy ra các ca sốt xuất huyết mới trong một khu vực và do đó giúp lập kế hoạch giám sát bệnh tật và kiểm soát véc tơ hiệu quả và sáng tạo hơn."} +{"idx": 1880109, "en": "BACKGROUND Due to the frequent reassortment and zoonotic potential of influenza A viruses, rapid gain of sequence information is crucial. Alongside established next-generation sequencing protocols, the MinION sequencing device (Oxford Nanopore Technologies) has become a serious competitor for routine whole-genome sequencing. Here, we established a novel, rapid and high-throughput MinION multiplexing workflow based on a universal RT-PCR. METHODS Twelve representative influenza A virus samples of multiple subtypes were universally amplified in a one-step RT-PCR and subsequently sequenced on the MinION instrument in conjunction with a barcoding library preparation kit from the rapid family and the MinIT performing live base-calling. The identical PCR products were sequenced on an IonTorrent platform and, after final consensus assembly, all data was compared for validation. To prove the practicability of the MinION-MinIT method in human and veterinary diagnostics, we sequenced recent and historical influenza strains for further benchmarking. RESULTS The MinION-MinIT combination generated over two million reads for twelve samples in a six-hour sequencing run, from which a total of 72% classified as quality screened, trimmed and mapped influenza reads to produce full genome sequences. Identities between the datasets of > 99.9% were achieved, with 100% coverage of all segments alongside a sufficient confidence and 4492fold mean depth. From RNA extraction to finished sequences, only 14 h were required. CONCLUSIONS Overall, we developed and validated a novel and rapid multiplex workflow for influenza A virus sequencing. This protocol suits both clinical and academic settings, aiding in real time diagnostics and passive surveillance.", "gemini": "BỐI CẢNH Do sự tái tổ hợp thường xuyên và tiềm năng lây truyền từ động vật sang người của vi-rút cúm A, việc thu thập nhanh chóng thông tin trình tự là rất quan trọng. Cùng với các giao thức giải trình tự thế hệ tiếp theo đã được thiết lập, thiết bị giải trình tự MinION (Oxford Nanopore Technologies) đã trở thành đối thủ cạnh tranh nghiêm túc cho việc giải trình tự toàn bộ hệ gen thông thường. Tại đây, chúng tôi thiết lập một quy trình làm việc ghép nối đa luồng MinION mới, nhanh chóng và thông lượng cao dựa trên RT-PCR phổ quát. PHƯƠNG PHÁP Mười hai mẫu vi-rút cúm A đại diện cho nhiều phân nhóm đã được khuếch đại phổ biến trong một RT-PCR một bước và sau đó được giải trình tự trên thiết bị MinION cùng với bộ dụng cụ chuẩn bị thư viện mã vạch từ họ nhanh và MinIT thực hiện gọi gốc trực tiếp. Các sản phẩm PCR giống hệt nhau đã được giải trình tự trên nền tảng IonTorrent và sau khi lắp ráp đồng thuận cuối cùng, tất cả dữ liệu đã được so sánh để xác nhận. Để chứng minh tính thực tiễn của phương pháp MinION-MinIT trong chẩn đoán cho người và thú y, chúng tôi đã giải trình tự các chủng cúm gần đây và trước đây để đánh giá chuẩn hơn nữa. KẾT QUẢ Sự kết hợp MinION-MinIT đã tạo ra hơn hai triệu lần đọc cho mười hai mẫu trong quá trình giải trình tự sáu giờ, trong đó tổng cộng 72% được phân loại là các lần đọc cúm được sàng lọc chất lượng, cắt và ánh xạ để tạo ra trình tự toàn bộ hệ gen. Các bản sắc giữa các bộ dữ liệu đạt > 99,9%, với độ phủ 100% của tất cả các phân đoạn cùng với độ tin cậy đủ và độ sâu trung bình là 4492 lần. Chỉ cần 14 giờ từ khi chiết xuất RNA đến khi có trình tự hoàn chỉnh. KẾT LUẬN Nhìn chung, chúng tôi đã phát triển và xác nhận một quy trình làm việc ghép nối đa luồng mới và nhanh chóng để giải trình tự vi-rút cúm A. Giao thức này phù hợp với cả các thiết lập lâm sàng và học thuật, hỗ trợ chẩn đoán thời gian thực và giám sát thụ động."} +{"idx": 1880110, "en": "BACKGROUND Health-related quality of life (HRQoL) has become a major endpoint to assess the clinical benefit of new therapeutic strategies in oncology clinical trials. Typically, HRQoL outcomes are analyzed using linear mixed models (LMMs). However, longitudinal analysis of HRQoL in the presence of missing data remains complex and unstandardized. Our objective was to compare the modeling alternatives that account for informative dropout. METHODS We investigated three alternative methods-the selection model (SM), pattern-mixture model (PMM), and shared-parameters model (SPM)-in relation to the LMM. We first compared them on the basis of methodological arguments highlighting their advantages and drawbacks. Then, we applied them to data from a randomized clinical trial that included 267 patients with advanced esophageal cancer for the analysis of four HRQoL dimensions evaluated using the European Organisation for Research and Treatment of Cancer (EORTC) QLQ-C30 questionnaire. RESULTS We highlighted differences in terms of outputs, interpretation, and underlying modeling assumptions; this methodological comparison could guide the choice of method according to the context. In the application, none of the four models detected a significant difference between the two treatment arms. The estimated effect of time on HRQoL varied according to the method: for all analyzed dimensions, the PMM estimated an effect that contrasted with those estimated by the SM and SPM; the LMM estimated effects were confirmed by the SM (on two of four HRQoL dimensions) and SPM (on three of four HRQoL dimensions). CONCLUSIONS The PMM, SM, or SPM should be used to confirm or invalidate the results of LMM analysis when informative dropout is suspected. Of these three alternative methods, the SPM appears to be the most interesting from both theoretical and practical viewpoints. TRIAL REGISTRATION This study is registered with ClinicalTrials.gov , number NCT00861094 .", "gemini": "BỐI CẢNH Chất lượng cuộc sống liên quan đến sức khỏe (HRQoL) đã trở thành một điểm cuối chính để đánh giá lợi ích lâm sàng của các chiến lược điều trị mới trong các thử nghiệm lâm sàng về ung thư. Thông thường, kết quả HRQoL được phân tích bằng các mô hình tuyến tính hỗn hợp (LMM). Tuy nhiên, việc phân tích theo chiều dọc của HRQoL khi có dữ liệu bị thiếu vẫn còn phức tạp và chưa được chuẩn hóa. Mục tiêu của chúng tôi là so sánh các phương pháp thay thế mô hình có tính đến việc bỏ học có thông tin. PHƯƠNG PHÁP Chúng tôi đã nghiên cứu ba phương pháp thay thế - mô hình lựa chọn (SM), mô hình hỗn hợp kiểu (PMM) và mô hình tham số chung (SPM) - liên quan đến LMM. Đầu tiên, chúng tôi so sánh chúng trên cơ sở các lập luận về phương pháp luận nêu bật ưu điểm và nhược điểm của chúng. Sau đó, chúng tôi áp dụng chúng vào dữ liệu từ một thử nghiệm lâm sàng ngẫu nhiên bao gồm 267 bệnh nhân ung thư thực quản tiến triển để phân tích bốn chiều HRQoL được đánh giá bằng bảng câu hỏi EORTC QLQ-C30 của Tổ chức Nghiên cứu và Điều trị Ung thư Châu Âu (EORTC). KẾT QUẢ Chúng tôi nêu bật sự khác biệt về mặt đầu ra, cách giải thích và các giả định mô hình cơ bản; so sánh phương pháp luận này có thể hướng dẫn lựa chọn phương pháp theo từng bối cảnh. Trong ứng dụng, không có mô hình nào trong bốn mô hình phát hiện thấy sự khác biệt đáng kể giữa hai nhóm điều trị. Ước tính tác động của thời gian đối với HRQoL thay đổi tùy theo phương pháp: đối với tất cả các chiều được phân tích, PMM ước tính một tác động trái ngược với các tác động ước tính bởi SM và SPM; các tác động ước tính của LMM đã được SM (trên hai trong bốn chiều HRQoL) và SPM (trên ba trong bốn chiều HRQoL) xác nhận. KẾT LUẬN Nên sử dụng PMM, SM hoặc SPM để xác nhận hoặc bác bỏ kết quả phân tích LMM khi nghi ngờ có tình trạng bỏ học có thông tin. Trong số ba phương pháp thay thế này, SPM có vẻ thú vị nhất cả về mặt lý thuyết và thực tiễn. ĐĂNG KÝ THỬ NGHIỆM Nghiên cứu này được đăng ký trên ClinicalTrials.gov, số NCT00861094."} +{"idx": 1880111, "en": "BACKGROUND The family cluster is one of most important modes of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission throughout China, and more details are needed about how family clusters cause the spread of coronavirus disease 2019 (COVID-19). CASE PRESENTATION We retrospectively reviewed 7 confirmed cases from one family cluster. Both clinical features and laboratory examination results were described. Patient 1 had been in close contact with someone who was later confirmed to have COVID-19 in Wuhan City before he returned back to his hometown. He had dinner with 6 other members in his family. All the persons developed COVID-19 successively except for one older woman who neither had dinner with them nor shared a sleeping room with her husband. Six patients had mild or moderate COVID-19 but one older man with underlying diseases progressed into the severe type. After general and symptomatic treatments, all the patients recovered. CONCLUSIONS In a family cluster, having dinner together may be an important mode for the transmission of SARS-CoV-2. In this setting, most cases are mild with a favorable prognosis, while elderly patients with underlying diseases may progress into the severe type. For someone who has close contact with a confirmed case, 14-day isolation is necessary to contain virus transmission.", "gemini": "BỐI CẢNH Nhóm gia đình là một trong những phương thức lây truyền hội chứng hô hấp cấp tính nặng do virus corona 2 (SARS-CoV-2) quan trọng nhất trên khắp Trung Quốc và cần có thêm nhiều thông tin chi tiết về cách các nhóm gia đình gây ra sự lây lan của bệnh do virus corona 2019 (COVID-19). TRÌNH BÀY CA LÂM SÀNG Chúng tôi đã xem xét lại 7 trường hợp được xác nhận từ một nhóm gia đình. Cả các đặc điểm lâm sàng và kết quả xét nghiệm đều được mô tả. Bệnh nhân 1 đã tiếp xúc gần với một người sau đó được xác nhận mắc COVID-19 tại thành phố Vũ Hán trước khi anh ta trở về quê nhà. Anh ấy đã ăn tối với 6 thành viên khác trong gia đình. Tất cả những người này đều mắc COVID-19 liên tiếp nhau ngoại trừ một phụ nữ lớn tuổi không ăn tối với họ cũng không ngủ chung phòng với chồng. Sáu bệnh nhân mắc COVID-19 nhẹ hoặc trung bình nhưng một người đàn ông lớn tuổi mắc các bệnh nền đã tiến triển thành dạng nặng. Sau khi điều trị chung và điều trị triệu chứng, tất cả các bệnh nhân đều hồi phục. KẾT LUẬN Trong một nhóm gia đình, ăn tối cùng nhau có thể là một phương thức lây truyền SARS-CoV-2 quan trọng. Trong bối cảnh này, hầu hết các trường hợp đều nhẹ với tiên lượng thuận lợi, trong khi bệnh nhân cao tuổi mắc các bệnh nền có thể tiến triển thành dạng nặng. Đối với người tiếp xúc gần với trường hợp được xác nhận, cần phải cách ly 14 ngày để ngăn chặn sự lây truyền vi-rút."} +{"idx": 1880112, "en": "BACKGROUND Factor XIII (FXIII) deficiency is an extremely rare bleeding disorder that is commonly due to mutations in the FXIIIA subunit gene (F13A1), and it has been reported to have a prevalence of one per 2 million. We describe a new genetic variant in the F13A1 gene that caused a patient to suffer from lifelong hemorrhagic diathesis. CASE PRESENTATION We evaluated a 20-year-old female with umbilical cord bleeding after birth, an intracerebral hemorrhage at age 6, and other bleeding episodes, including hematuria and cephalohematoma, who suffered from a lifelong hemorrhagic diathesis. The clot solubility test showed that the clot of the patient was dissolved in urea solution at 10 h. Genetic testing identified a novel homozygous mutation, c.984C > A(p. Cys328stop), resulting in a premature stop codon in exon 8 of the F13A1 gene. The results obtained with ClusterX software showed that Cys328 of exon 8 in the F13A1 gene is highly conserved among species. CONCLUSION We reported a novel homozygous mutation in the F13A1 gene in a factor XIII (FXIII)-deficient patient, which adds a new point mutation to the mutant library. In this paper, we discuss other aspects of the disease, including laboratory examination, homogeneous sequence alignment and molecular modeling.", "gemini": "BỐI CẢNH Thiếu hụt yếu tố XIII (FXIII) là một rối loạn chảy máu cực kỳ hiếm gặp thường do đột biến ở gen tiểu đơn vị FXIIIA (F13A1) và được báo cáo có tỷ lệ mắc là một trên 2 triệu. Chúng tôi mô tả một biến thể di truyền mới trong gen F13A1 khiến bệnh nhân bị bệnh xuất huyết di truyền suốt đời. TRÌNH BÀY CA LÂM SÀNG Chúng tôi đã đánh giá một phụ nữ 20 tuổi bị chảy máu dây rốn sau khi sinh, xuất huyết não ở tuổi 6 và các cơn chảy máu khác, bao gồm tiểu máu và máu tụ dưới da đầu, người bị bệnh xuất huyết di truyền suốt đời. Xét nghiệm độ tan của cục máu đông cho thấy cục máu đông của bệnh nhân tan trong dung dịch urê sau 10 giờ. Xét nghiệm di truyền xác định một đột biến đồng hợp tử mới, c.984C>A(p.Cys328stop), dẫn đến mã dừng sớm ở exon 8 của gen F13A1. Kết quả thu được bằng phần mềm ClusterX cho thấy Cys328 của exon 8 trong gen F13A1 được bảo tồn cao giữa các loài. KẾT LUẬN Chúng tôi đã báo cáo một đột biến đồng hợp tử mới trong gen F13A1 ở một bệnh nhân thiếu yếu tố XIII (FXIII), bổ sung một điểm đột biến mới vào thư viện đột biến. Trong bài báo này, chúng tôi thảo luận về các khía cạnh khác của bệnh, bao gồm xét nghiệm, căn chỉnh trình tự đồng nhất và mô hình phân tử."} +{"idx": 1880113, "en": "BACKGROUND Caesarean scar pregnancy (CSP) is a rare complication of caesarean delivery and a special type of ectopic pregnancy. Gestational trophoblastic neoplasia (GTN) is an uncommon complication of pregnancy. Early diagnosis of the two diseases is crucial because a delay or misdiagnosis can lead to increased maternal morbidity and mortality. CASE PRESENTATION We report two cases of uterine isthmus lesions with a previous caesarean section (CS). Two patients were misdiagnosed based on the first ultrasound exam. The first case of trophoblastic tumour was initially diagnosed as CSP, while the second case, which had a scar pregnancy, was misdiagnosed as GTN. The misdiagnoses were due to the particularity of the locations of the lesions in the two patients, complicating the ultrasound-based diagnosis and hindering early clinical diagnosis and treatment. CONCLUSIONS A medical history, β-hCG measurements and transvaginal ultrasound are necessary to diagnose lesions in the lower anterior wall of the uterus early. However, when the location cannot be determined, magnetic resonance imaging (MRI) can be further performed to determine whether the lesion is located at the uterine scar. Combined with the degree of increased β-hCG, differentiate CSP, myometrial GTN or caesarean scar GTN is helpful.", "gemini": "BỐI CẢNH Mang thai sẹo mổ lấy thai (CSP) là một biến chứng hiếm gặp của việc sinh mổ và là một loại thai ngoài tử cung đặc biệt. U nguyên bào nuôi thai nghén (GTN) là một biến chứng không phổ biến của thai kỳ. Việc chẩn đoán sớm hai bệnh này là rất quan trọng vì chẩn đoán chậm hoặc chẩn đoán nhầm có thể dẫn đến tăng tỷ lệ mắc bệnh và tử vong ở bà mẹ. TRÌNH BÀY CA LÂM SÀNG Chúng tôi báo cáo hai trường hợp tổn thương eo tử cung có tiền sử mổ lấy thai (CS). Hai bệnh nhân được chẩn đoán nhầm dựa trên lần siêu âm đầu tiên. Trường hợp đầu tiên của khối u nguyên bào nuôi ban đầu được chẩn đoán là CSP, trong khi trường hợp thứ hai, mang thai sẹo mổ, được chẩn đoán nhầm là GTN. Các chẩn đoán nhầm là do đặc điểm riêng của vị trí tổn thương ở hai bệnh nhân, làm phức tạp chẩn đoán dựa trên siêu âm và cản trở chẩn đoán và điều trị lâm sàng sớm. KẾT LUẬN Tiền sử bệnh, đo β-hCG và siêu âm qua ngã âm đạo là cần thiết để chẩn đoán sớm các tổn thương ở thành trước dưới của tử cung. Tuy nhiên, khi không xác định được vị trí, có thể tiến hành chụp cộng hưởng từ (MRI) để xác định xem tổn thương có nằm ở sẹo tử cung hay không. Kết hợp với mức tăng β-hCG, phân biệt CSP, GTN cơ tử cung hoặc GTN sẹo mổ là rất hữu ích."} +{"idx": 1880114, "en": "BACKGROUND China's smart home for elderly care emerged in 2008, and had went through four developmental stages which consists of seed stage, start-up stage, development stage and popularization stage. MAIN TEXT The status quo and development of smart home for elderly care in China is reviewed, and suggestions are provided on how to further develop China's smart home for elderly care. The focus of China's policies on smart home for elderly care were different during those four developmental stages. Compared with Western countries, China's smart home for elderly care is a policy-driven product rather than technology-driven or demand-driven one. In addition, it is quasi-public goods rather than private goods. These unique characteristics of China's smart home for elderly care not only become the driving force of its rapid development, but also bring many challenges to its development, such as the insufficient demand, the disorderly development, and the waste of public and private resources. CONCLUSIONS Although great progress has been made in China's smart home care, much efforts are still needed to further advance its development. The technical standards for the elderly care services should be formulated as soon as possible and the existing public and private smart home for elderly care platforms should be combined. Enterprises involved in smart home care services should be encouraged to develop new technologies to reduce the cost of products and services provided by smart home for elderly care.", "gemini": "BỐI CẢNH Nhà thông minh chăm sóc người cao tuổi của Trung Quốc xuất hiện vào năm 2008 và đã trải qua bốn giai đoạn phát triển bao gồm giai đoạn hạt giống, giai đoạn khởi nghiệp, giai đoạn phát triển và giai đoạn phổ biến. NỘI DUNG CHÍNH Tình hình hiện tại và sự phát triển của nhà thông minh chăm sóc người cao tuổi ở Trung Quốc được xem xét và đưa ra các đề xuất về cách phát triển hơn nữa nhà thông minh chăm sóc người cao tuổi của Trung Quốc. Trọng tâm của các chính sách của Trung Quốc về nhà thông minh chăm sóc người cao tuổi là khác nhau trong bốn giai đoạn phát triển đó. So với các nước phương Tây, nhà thông minh chăm sóc người cao tuổi của Trung Quốc là một sản phẩm do chính sách thúc đẩy chứ không phải do công nghệ thúc đẩy hoặc do nhu cầu thúc đẩy. Ngoài ra, nó là hàng hóa bán công chứ không phải hàng hóa tư nhân. Những đặc điểm độc đáo này của nhà thông minh chăm sóc người cao tuổi của Trung Quốc không chỉ trở thành động lực thúc đẩy sự phát triển nhanh chóng của nó mà còn mang lại nhiều thách thức cho sự phát triển của nó, chẳng hạn như nhu cầu không đủ, sự phát triển hỗn loạn và lãng phí nguồn lực công và tư. KẾT LUẬN Mặc dù đã có nhiều tiến bộ trong dịch vụ chăm sóc tại nhà thông minh của Trung Quốc, nhưng vẫn cần nhiều nỗ lực hơn nữa để thúc đẩy sự phát triển của dịch vụ này. Các tiêu chuẩn kỹ thuật cho các dịch vụ chăm sóc người cao tuổi nên được xây dựng càng sớm càng tốt và các nền tảng nhà thông minh chăm sóc người cao tuổi công cộng và tư nhân hiện có nên được kết hợp. Các doanh nghiệp tham gia vào các dịch vụ chăm sóc tại nhà thông minh nên được khuyến khích phát triển các công nghệ mới để giảm chi phí cho các sản phẩm và dịch vụ do nhà thông minh chăm sóc người cao tuổi cung cấp."} +{"idx": 1880115, "en": "BACKGROUND Screening for early detection of lung cancer has been performed in high-risk individuals with smoking history. However, researches on the distribution, clinical characteristics, and prognosis of these high-risk individuals in an actual cohort are lacking. Thus, the objective of this study was to retrospectively review characteristics and prognosis of patients with smoking history in an actual lung cancer cohort. METHODS The present study used the lung cancer cohort of the Catholic Medical Centers at the Catholic University of Korea from 2014 to 2017. Patients with non-small cell lung cancer were enrolled. They were categorized into high and low-risk groups based on their smoking history using the national lung screening trial guideline. Distribution, clinical characteristics, and survival data of each group were estimated. RESULTS Of 439 patients, 223 (50.8%) patients were in the high-risk group. Patients in the high-risk group had unfavorable clinical characteristics and tumor biologic features. Overall survival of the high-risk group was significantly shorter than that of the low-risk group with both early (I, II) and advanced stages (III, IV). In multivariate analysis, heavy smoking remained one of the most important poor clinical prognostic factors in patients with lung cancer. It showed a dose-dependent relationship with patients' survival. CONCLUSIONS High-risk individuals had poor clinical outcomes. Patients' prognosis seemed to be deteriorated as smoking amount increased. Therefore, active screening and clinical attention are needed for high-risk individuals.", "gemini": "BỐI CẢNH Việc sàng lọc để phát hiện sớm ung thư phổi đã được thực hiện ở những người có nguy cơ cao có tiền sử hút thuốc. Tuy nhiên, các nghiên cứu về phân bố, đặc điểm lâm sàng và tiên lượng của những người có nguy cơ cao này trong một nhóm thực tế còn thiếu. Do đó, mục tiêu của nghiên cứu này là xem xét lại đặc điểm và tiên lượng của những bệnh nhân có tiền sử hút thuốc trong một nhóm ung thư phổi thực tế. PHƯƠNG PHÁP Nghiên cứu hiện tại sử dụng nhóm ung thư phổi của Trung tâm Y tế Công giáo tại Đại học Công giáo Hàn Quốc từ năm 2014 đến năm 2017. Những bệnh nhân bị ung thư phổi không phải tế bào nhỏ đã được ghi danh. Họ được phân loại vào nhóm có nguy cơ cao và nguy cơ thấp dựa trên tiền sử hút thuốc của họ bằng cách sử dụng hướng dẫn thử nghiệm sàng lọc phổi quốc gia. Phân bố, đặc điểm lâm sàng và dữ liệu sống sót của từng nhóm được ước tính. KẾT QUẢ Trong số 439 bệnh nhân, có 223 (50,8%) bệnh nhân thuộc nhóm nguy cơ cao. Những bệnh nhân trong nhóm nguy cơ cao có các đặc điểm lâm sàng và đặc điểm sinh học của khối u không thuận lợi. Tỷ lệ sống sót chung của nhóm nguy cơ cao ngắn hơn đáng kể so với nhóm nguy cơ thấp ở cả giai đoạn sớm (I, II) và giai đoạn tiến triển (III, IV). Trong phân tích đa biến, hút thuốc nhiều vẫn là một trong những yếu tố tiên lượng lâm sàng kém quan trọng nhất ở những bệnh nhân ung thư phổi. Nó cho thấy mối quan hệ phụ thuộc vào liều lượng với sự sống còn của bệnh nhân. KẾT LUẬN Những người có nguy cơ cao có kết quả lâm sàng kém. Tiên lượng của bệnh nhân dường như xấu đi khi lượng thuốc lá tăng. Do đó, cần sàng lọc tích cực và chú ý lâm sàng đối với những người có nguy cơ cao."} +{"idx": 1880116, "en": "BACKGROUND Polypharmacy is a key challenge in healthcare especially in older and multimorbid patients. The use of multiple medications increases the potential for drug interactions and for prescription of potentially inappropriate medications. eHealth solutions are increasingly recommended in healthcare, with big data analysis techniques as a major component. In the following we use the term analysis of big data as referring to the computational analysis of large data sets to find patterns, trends, and associations in large data sets collected from a wide range of sources in contrast to using classical statistics programs. It is hypothesized that big data analysis is able to reveal patterns in patient data that would not be identifiable using conventional methods of data analysis. The aim of this review was to evaluate whether there are existing big data analysis techniques that can help to identify patients consuming multiple drugs and to assist in the reduction of polypharmacy in patients. METHODS A computerized search was conducted in February 2019 and updated in May 2020, using the PubMed, Web of Science and Cochrane Library databases. The search strategy was defined by the principles of a systematic search, using the PICO scheme. All studies evaluating big data analytics about patients consuming multiple drugs were considered. Two researchers assessed all search results independently to identify eligible studies. The data was then extracted into standardized tables. RESULTS A total of 327 studies were identified through the database search. After title and abstract screening, 302 items were removed. Only three studies were identified as addressing big data analysis techniques in patients with polypharmacy. One study extracted antipsychotic polypharmacy data, the second introduced a decision support system to evaluate side-effects in patients with polypharmacy and the third evaluated a decision support system to identify polypharmacy-related problems in individuals. CONCLUSIONS There are few studies to date which have used big data analysis techniques for identification and management of polypharmacy. There may be a need to further explore interdisciplinary collaboration between computer scientists and healthcare professionals, to develop and evaluate big data analysis techniques that can be implemented to manage polypharmacy.", "gemini": "BỐI CẢNH Đa dược là một thách thức chính trong chăm sóc sức khỏe, đặc biệt là ở những bệnh nhân lớn tuổi và mắc nhiều bệnh. Việc sử dụng nhiều loại thuốc làm tăng khả năng xảy ra tương tác thuốc và kê đơn các loại thuốc có khả năng không phù hợp. Các giải pháp eHealth ngày càng được khuyến nghị trong chăm sóc sức khỏe, với các kỹ thuật phân tích dữ liệu lớn là một thành phần chính. Trong phần sau, chúng tôi sử dụng thuật ngữ phân tích dữ liệu lớn để chỉ việc phân tích tính toán các tập dữ liệu lớn để tìm ra các mẫu hình, xu hướng và mối liên hệ trong các tập dữ liệu lớn được thu thập từ nhiều nguồn khác nhau, trái ngược với việc sử dụng các chương trình thống kê cổ điển. Người ta đưa ra giả thuyết rằng phân tích dữ liệu lớn có khả năng tiết lộ các mẫu hình trong dữ liệu bệnh nhân mà không thể xác định được bằng các phương pháp phân tích dữ liệu thông thường. Mục đích của bài đánh giá này là đánh giá liệu có các kỹ thuật phân tích dữ liệu lớn hiện có nào có thể giúp xác định những bệnh nhân sử dụng nhiều loại thuốc và hỗ trợ giảm tình trạng đa dược ở bệnh nhân hay không. PHƯƠNG PHÁP Một cuộc tìm kiếm trên máy tính đã được tiến hành vào tháng 2 năm 2019 và được cập nhật vào tháng 5 năm 2020, sử dụng các cơ sở dữ liệu PubMed, Web of Science và Cochrane Library. Chiến lược tìm kiếm được xác định bởi các nguyên tắc của tìm kiếm có hệ thống, sử dụng lược đồ PICO. Tất cả các nghiên cứu đánh giá phân tích dữ liệu lớn về những bệnh nhân sử dụng nhiều loại thuốc đều được xem xét. Hai nhà nghiên cứu đã đánh giá độc lập tất cả các kết quả tìm kiếm để xác định các nghiên cứu đủ điều kiện. Sau đó, dữ liệu được trích xuất vào các bảng chuẩn hóa. KẾT QUẢ Tổng cộng có 327 nghiên cứu được xác định thông qua tìm kiếm cơ sở dữ liệu. Sau khi sàng lọc tiêu đề và tóm tắt, 302 mục đã bị loại bỏ. Chỉ có ba nghiên cứu được xác định là giải quyết các kỹ thuật phân tích dữ liệu lớn ở những bệnh nhân đa dược. Một nghiên cứu đã trích xuất dữ liệu đa dược chống loạn thần, nghiên cứu thứ hai giới thiệu một hệ thống hỗ trợ quyết định để đánh giá tác dụng phụ ở những bệnh nhân đa dược và nghiên cứu thứ ba đánh giá một hệ thống hỗ trợ quyết định để xác định các vấn đề liên quan đến đa dược ở các cá nhân. KẾT LUẬN Cho đến nay, có rất ít nghiên cứu sử dụng các kỹ thuật phân tích dữ liệu lớn để xác định và quản lý tình trạng đa dược. Có thể cần phải tiếp tục khám phá sự hợp tác liên ngành giữa các nhà khoa học máy tính và các chuyên gia chăm sóc sức khỏe để phát triển và đánh giá các kỹ thuật phân tích dữ liệu lớn có thể được triển khai để quản lý tình trạng đa dược."} +{"idx": 1880117, "en": "BACKGROUND Both the 7th and 8th editions of the American Joint Committee on Cancer (AJCC) staging system for perihilar cholangiocarcinoma (pCCA) had the same definition for T2a and T2b. But the value of this classification as prognostic factor remains unclear. METHODS 178 patients with stage T2a or T2b who underwent curative intent resection for pCCA between Jan 2010 and Dec 2018 were enrolled. Relationships between survival and clinicopathological factors including patient demographics and tumor characteristics were evaluated using univariate and multivariate Cox regression analysis. The overall survival (OS) were calculated by Kaplan-Meier method. RESULTS There was no significant difference in OS between T2a and T2b groups, and the median OS duration were 37 and 31 months (P = 0.354). Both the 7th and 8th edition of the AJCC TNM staging demonstrated a poor prognostic predictive performance. High level of preoperative AST (≥85.0 IU/L) and CA19-9 (≥1000 U/mL), vascular resection and lower pathological differentiation of the tumor were the independent predictors for poor survival after resection. CONCLUSION The newly released 8th edition of AJCC staging system demonstrated a poor ability to discriminate the prognosis of patients with stage T2a and T2b pCCA after resection.", "gemini": "BỐI CẢNH Cả phiên bản thứ 7 và thứ 8 của hệ thống phân loại giai đoạn của Ủy ban Ung thư Hoa Kỳ (AJCC) cho ung thư biểu mô đường mật quanh gan (pCCA) đều có cùng định nghĩa cho T2a và T2b. Nhưng giá trị của phân loại này như một yếu tố tiên lượng vẫn chưa rõ ràng. PHƯƠNG PHÁP 178 bệnh nhân giai đoạn T2a hoặc T2b đã được cắt bỏ khối u với mục đích chữa khỏi đối với pCCA trong khoảng thời gian từ tháng 1 năm 2010 đến tháng 12 năm 2018 đã được ghi danh. Mối quan hệ giữa tỷ lệ sống sót và các yếu tố lâm sàng và bệnh lý bao gồm thông tin nhân khẩu học của bệnh nhân và đặc điểm khối u đã được đánh giá bằng phân tích hồi quy Cox đơn biến và đa biến. Tỷ lệ sống toàn thể (OS) được tính bằng phương pháp Kaplan-Meier. KẾT QUẢ Không có sự khác biệt đáng kể về OS giữa các nhóm T2a và T2b và thời gian OS trung bình là 37 và 31 tháng (P = 0,354). Cả phiên bản thứ 7 và thứ 8 của phân loại giai đoạn TNM của AJCC đều cho thấy khả năng dự đoán tiên lượng kém. Nồng độ AST trước phẫu thuật cao (≥85,0 IU/L) và CA19-9 (≥1000 U/mL), cắt bỏ mạch máu và biệt hóa bệnh lý thấp hơn của khối u là những yếu tố dự báo độc lập cho tỷ lệ sống sót kém sau khi cắt bỏ. KẾT LUẬN Phiên bản thứ 8 mới được phát hành của hệ thống phân loại giai đoạn AJCC cho thấy khả năng phân biệt tiên lượng kém của bệnh nhân pCCA giai đoạn T2a và T2b sau khi cắt bỏ."} +{"idx": 1880118, "en": "BACKGROUND Cell-in-cell structures (caused by cell cannibalistic activity) have been related to prognosis of many cancers. This is the first multi-institutional study to assess the prognostic impact of cell-in-cell structures in a large cohort of early oral tongue squamous cell carcinomas (OTSCC). METHODS A total of 308 cases from five Finnish University Hospitals and from the A.C. Camargo Cancer Center, São Paulo, Brazil, were included in this study. Cell-in-cell structures were evaluated on surgical postoperative sections that stained with hematoxylin and eosin staining. RESULTS We found that cell-in-cell structures associated with cancer-related mortality in univariable analysis with a hazard ratio (HR) of 2.99 (95%CI 1.52-5.88; P = 0.001). This association was confirmed in multivariable analysis (HR 2.22, 95%CI 1.12-4.44; P = 0.024). In addition, statistically significant associations were observed between the cell-in-cell structures and other adverse histopathologic characteristics including deep invasion (P <  0.001), high index of tumor budding (P = 0.007), worst pattern of invasion (P <  0.001), perineural invasion (P = 0.01), and stroma-rich pattern (P = 0.001). CONCLUSIONS Our findings demonstrate a significant relationship between cell-in-cell formation and aggressive characteristics of early OTSCC. Cell-in-cell structures have a distinct impact as a novel prognostic indicator in early OTSCC and they can be easily assessed during routine pathology practice.", "gemini": "BỐI CẢNH Các cấu trúc tế bào trong tế bào (do hoạt động ăn thịt tế bào) có liên quan đến tiên lượng của nhiều loại ung thư. Đây là nghiên cứu đa trung tâm đầu tiên đánh giá tác động tiên lượng của các cấu trúc tế bào trong tế bào trong một nhóm lớn các ung thư biểu mô tế bào vảy ở lưỡi giai đoạn sớm (OTSCC). PHƯƠNG PHÁP Tổng cộng 308 trường hợp từ năm Bệnh viện Đại học Phần Lan và từ Trung tâm Ung thư A.C. Camargo, São Paulo, Brazil, đã được đưa vào nghiên cứu này. Các cấu trúc tế bào trong tế bào được đánh giá trên các phần sau phẫu thuật được nhuộm bằng phương pháp nhuộm hematoxylin và eosin. KẾT QUẢ Chúng tôi thấy rằng các cấu trúc tế bào trong tế bào liên quan đến tỷ lệ tử vong do ung thư trong phân tích đơn biến với tỷ lệ nguy cơ (HR) là 2,99 (95% CI 1,52-5,88; P = 0,001). Sự liên kết này đã được xác nhận trong phân tích đa biến (HR 2,22, 95% CI 1,12-4,44; P = 0,024). Ngoài ra, các mối liên hệ có ý nghĩa thống kê đã được quan sát thấy giữa các cấu trúc tế bào trong tế bào và các đặc điểm mô bệnh học bất lợi khác bao gồm xâm lấn sâu (P < 0,001), chỉ số nảy chồi khối u cao (P = 0,007), kiểu xâm lấn tệ nhất (P < 0,001), xâm lấn quanh dây thần kinh (P = 0,01) và kiểu giàu chất nền (P = 0,001). KẾT LUẬN Những phát hiện của chúng tôi chứng minh mối quan hệ đáng kể giữa sự hình thành tế bào trong tế bào và các đặc điểm hung hăng của OTSCC giai đoạn sớm. Các cấu trúc tế bào trong tế bào có tác động riêng biệt như một chỉ báo tiên lượng mới trong OTSCC giai đoạn sớm và chúng có thể được đánh giá dễ dàng trong quá trình thực hành bệnh lý thông thường."} +{"idx": 1880119, "en": "BACKGROUND Astrocytoma is a common tumor type in primary central nervous system and has a high death rate around the world. Aberrant expression of long non-coding RNAs (lncRNAs) has been introduced by emerging studies to result in the development of diverse cancers. METHODS RT-qPCR examined the expression of SNHG17, miR-876-5p and ERLIN2, and western blot evaluated ERLIN2 protein level. RNA pull down and luciferase reporter assays illustrated the relationships between SNHG17 and its downstream molecules. RESULTS SNHG17 was up-regulated in astrocytoma cells. Moreover, SNHG17 silence could repress the proliferation, migration and invasion of astrocytoma cells. Besides, miR-876-5p was selected out as a downstream molecule of SNHG17 in astrocytoma. ERLIN2 was determined to be targeted by miR-876-5p. ERLIN2 mRNA and protein levels were lessened by miR-876-5p overexpression and SNHG17 silence. Additionally, miR-876-5p overexpression decelerated the biological processes of astrocytoma cells, so did ERLIN2 knockdown. More importantly, the impacts of SNHG17 down-regulation on the malignant behaviors of astrocytoma cells were counteracted by overexpressed ERLIN2 or inhibited miR-876-5p. CONCLUSIONS SNHG17 could induce the progression of astrocytoma by sponging miR-876-5p to elevate the expression of ERLIN2. This study indicated that SNHG17 has a high potential to be a therapeutic target for astrocytoma.", "gemini": "BỐI CẢNH U sao bào là một loại khối u phổ biến trong hệ thần kinh trung ương nguyên phát và có tỷ lệ tử vong cao trên toàn thế giới. Các nghiên cứu mới nổi đã đưa ra biểu hiện bất thường của các RNA không mã hóa dài (lncRNA) dẫn đến sự phát triển của nhiều loại ung thư khác nhau. PHƯƠNG PHÁP RT-qPCR đã kiểm tra biểu hiện của SNHG17, miR-876-5p và ERLIN2, và phương pháp Western blot đã đánh giá mức protein ERLIN2. Các xét nghiệm kéo RNA xuống và báo cáo luciferase minh họa mối quan hệ giữa SNHG17 và các phân tử hạ nguồn của nó. KẾT QUẢ SNHG17 được điều hòa tăng lên trong các tế bào u sao bào. Hơn nữa, sự im lặng của SNHG17 có thể kìm hãm sự tăng sinh, di chuyển và xâm lấn của các tế bào u sao bào. Bên cạnh đó, miR-876-5p được chọn là phân tử hạ nguồn của SNHG17 trong u sao bào. ERLIN2 được xác định là mục tiêu của miR-876-5p. Mức mRNA và protein ERLIN2 giảm xuống do sự biểu hiện quá mức của miR-876-5p và sự im lặng của SNHG17. Ngoài ra, sự biểu hiện quá mức của miR-876-5p làm chậm các quá trình sinh học của các tế bào u sao bào, cũng như việc hạ gục ERLIN2. Quan trọng hơn, tác động của việc điều hòa giảm SNHG17 đối với các hành vi ác tính của các tế bào u sao bào đã được chống lại bởi ERLIN2 được biểu hiện quá mức hoặc miR-876-5p bị ức chế. KẾT LUẬN SNHG17 có thể gây ra sự tiến triển của u sao bào bằng cách hấp thụ miR-876-5p để nâng cao biểu hiện của ERLIN2. Nghiên cứu này chỉ ra rằng SNHG17 có tiềm năng cao trở thành mục tiêu điều trị cho u sao bào."} +{"idx": 1880120, "en": "BACKGROUND Breast cancer (BC) is a malignant tumor that occurs in the epithelial tissue of the breast gland. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 3 (SNHG3) has been found to promote BC cell proliferation and invasion by regulating the microRNA (miR)-101/zinc-finger enhancer binding axis in BC. Herein, the objective of the present study is to evaluate the effect of lncRNA SNHG3 on BC cell proliferation and metastasis with the Notch signaling pathway. METHODS Differentially expressed lncRNA in BC tissues and normal breast tissues was analyzed. SNHG3 si-RNA-1 and SNHG3 si-RNA-2 were constructed to detect the mechanism of SNHG3 interference in BC cell proliferation, viability, migration and invasion. Then, dual-luciferase reporter gene assay was utilized to verify the binding relation between SNHG3 and miR-154-3p as well as miR-154-3p and Notch2. Moreover, xenograft transplantation was applied to confirm the in vitro experiments. RESULTS Highly expressed SNHG3 was observed in BC tissues. The growth of BC cells in vivo and in vitro was evidently repressed after silencing SNHG3. BC cell invasion and migration were inhibited by silencing SNHG3 in vitro. SNHG3 could act as a competing endogenous RNA of miR-154-3p and upregulate the Notch signaling pathway to promote BC cell development. Activation of the Notch signaling pathway can partly reverse the inhibition of cell activity induced by silencing SNHG3. CONCLUSION Our study demonstrated that interfered lncRNA SNHG3 promoted BC cell proliferation and metastasis by activating the Notch signaling pathway. This investigation may offer new insight for BC treatment.", "gemini": "BỐI CẢNH Ung thư vú (BC) là một khối u ác tính xảy ra ở mô biểu của tuyến vú. RNA không mã hóa dài (lncRNA) gen vật chủ RNA nhân nhỏ 3 (SNHG3) đã được phát hiện thúc đẩy sự phát triển và xâm lấn tế bào BC bằng cách điều chỉnh trục liên kết tăng cường microRNA (miR) -101 / kẽm-ngón trong BC. Ở đây, mục tiêu của nghiên cứu hiện tại là đánh giá tác dụng của lncRNA SNHG3 đối với sự phát triển và di căn của tế bào BC với con đường truyền tín hiệu Notch. PHƯƠNG PHÁP LncRNA biểu hiện khác biệt trong các mô BC và các mô vú bình thường đã được phân tích. SNHG3 si-RNA-1 và SNHG3 si-RNA-2 được xây dựng để phát hiện cơ chế can thiệp SNHG3 vào sự phát triển, khả năng sống, di chuyển và xâm lấn của tế bào BC. Sau đó, thử nghiệm gen phóng viên kép luciferase đã được sử dụng để xác minh mối quan hệ liên kết giữa SNHG3 và miR-154-3p cũng như miR-154-3p và Notch2. Hơn nữa, ghép xenograft đã được áp dụng để xác nhận các thí nghiệm trong ống nghiệm. KẾT QUẢ SNHG3 biểu hiện cao đã được quan sát thấy trong các mô BC. Sự phát triển của các tế bào BC trong ống nghiệm và trong ống nghiệm đã bị ức chế rõ ràng sau khi làm im lặng SNHG3. Sự xâm lấn và di chuyển của tế bào BC đã bị ức chế bằng cách làm im lặng SNHG3 trong ống nghiệm. SNHG3 có thể hoạt động như một RNA nội sinh cạnh tranh của miR-154-3p và tăng cường con đường truyền tín hiệu Notch để thúc đẩy sự phát triển của tế bào BC. Kích hoạt con đường truyền tín hiệu Notch có thể đảo ngược một phần sự ức chế hoạt động của tế bào gây ra bởi sự im lặng SNHG3. KẾT LUẬN Nghiên cứu của chúng tôi chứng minh rằng lncRNA SNHG3 bị can thiệp thúc đẩy sự phát triển và di căn của tế bào BC bằng cách kích hoạt con đường truyền tín hiệu Notch. Cuộc điều tra này có thể cung cấp cái nhìn sâu sắc mới cho việc điều trị BC."} +{"idx": 1880121, "en": "BACKGROUND Genomic instability is a hallmark of cancer. The G1 checkpoint allows cells to repair damaged DNA that may lead to genomic instability. The high-risk human papillomavirus (HPV) E7 gene can abrogate the G1 checkpoint, yet the mechanism is still not fully understood. Our recent study showed that WDHD1 (WD repeat and high mobility group [HMG]-box DNA-binding protein 1) plays a role in regulating G1 checkpoint of E7 expressing cells. In this study, we explored the mechanism by which WDHD1 regulates G1 checkpoint in HPV E7 expressing cells. METHODS NIKS and RPE1 derived cell lines were used. Real-time PCR, Rescue experiment, FACS and BrdU labeling experiments were performed to examine role of GCN5 in G1 checkpoint abrogation in HPV-16 E7 expressing cells. RESULTS In this study, we observed that WDHD1 facilitates G1 checkpoint abrogation by modulating GCN5 in HPV E7 expressing cells. Notably, depletion of WDHD1 caused G1 arrest while overexpression of GCN5 rescued the inhibitory effects of WDHD1 knockdown on G1/S progression. Furthermore, siWDHD1 significantly decreased cell cycle proliferation and DNA synthesis that was correlated with Akt phosphorylation (p-Akt), which was reversed by GCN5 overexpression in HPV E7 expressing cells. CONCLUSIONS In summary, our data identified a WDHD1/GCN5/Akt pathway leading to the abrogation of G1 checkpoint in the presence of damaged DNA, which may cause genomic instability and eventually HPV induced tumorigenesis.", "gemini": "BỐI CẢNH Sự bất ổn về bộ gen là một đặc điểm của bệnh ung thư. Điểm kiểm soát G1 cho phép các tế bào sửa chữa DNA bị hư hỏng có thể dẫn đến sự bất ổn về bộ gen. Gen E7 của vi-rút papilloma ở người (HPV) có nguy cơ cao có thể hủy bỏ điểm kiểm soát G1, nhưng cơ chế này vẫn chưa được hiểu đầy đủ. Nghiên cứu gần đây của chúng tôi cho thấy WDHD1 (protein liên kết DNA có hộp lặp lại WD và nhóm di động cao [HMG]-1) đóng vai trò trong việc điều hòa điểm kiểm soát G1 của các tế bào biểu hiện E7. Trong nghiên cứu này, chúng tôi đã khám phá cơ chế mà WDHD1 điều chỉnh điểm kiểm soát G1 trong các tế bào biểu hiện HPV E7. PHƯƠNG PHÁP Các dòng tế bào có nguồn gốc từ NIKS và RPE1 đã được sử dụng. Thực hiện phản ứng PCR thời gian thực, thí nghiệm giải cứu, FACS và thí nghiệm đánh dấu BrdU để kiểm tra vai trò của GCN5 trong việc hủy bỏ điểm kiểm soát G1 trong các tế bào biểu hiện HPV-16 E7. KẾT QUẢ Trong nghiên cứu này, chúng tôi quan sát thấy rằng WDHD1 tạo điều kiện cho việc hủy bỏ điểm kiểm soát G1 bằng cách điều chỉnh GCN5 trong các tế bào biểu hiện HPV E7. Đáng chú ý, sự suy giảm WDHD1 gây ra sự bắt giữ G1 trong khi sự biểu hiện quá mức của GCN5 giải cứu được các tác dụng ức chế của việc giảm WDHD1 trên sự tiến triển G1 / S. Hơn nữa, siWDHD1 làm giảm đáng kể sự gia tăng chu kỳ tế bào và tổng hợp DNA có liên quan đến sự phosphoryl hóa Akt (p-Akt), được đảo ngược bởi sự biểu hiện quá mức của GCN5 trong các tế bào biểu hiện HPV E7. KẾT LUẬN Tóm lại, dữ liệu của chúng tôi xác định một con đường WDHD1 / GCN5 / Akt dẫn đến việc hủy bỏ điểm kiểm soát G1 khi có DNA bị hư hỏng, có thể gây ra sự bất ổn về bộ gen và cuối cùng là quá trình hình thành khối u do HPV gây ra."} +{"idx": 1880122, "en": "BACKGROUND Interaction between microenvironment and breast cancer cells often is not considered at the early stages of drug development leading to failure of many drugs at later clinical stages. Etanercept is a TNF-alpha inhibitor that has been investigated for potential antitumor effect in breast cancer with conflicting results. METHODS Secretome data on MDA-MB-231 cancer cell-line were from public repositories and subjected to gene enrichment analyses. Since MDA-MB-231 cells secrete high levels of Granulocyte-Monocyte Colony Stimulating Factor, which activates macrophages to promote tumor growth, the effect of macrophage co-culturing on anticancer efficacy of Etanercept in breast cancer was evaluated using the Boolean network modeling and in vitro experiments including invasion, cell cycle, Annexin PI, and tetrazolium based viability assays and NFKB activity. RESULTS The secretome profile of MDA-MB-231 cells was similar to the expression of genes following treatment of breast cancer cells with TNF-α. Accordingly, inhibition of TNF-α by Etanercept decreased MDA-MB-231 cell survival, induced apoptosis and cell cycle arrest in vitro and inhibited NFKB activation. The inhibitory effect of Etanercept on cell viability, cell cycle progression, invasion and induction of apoptosis decreased following co-culturing of the cancer cells with macrophages. The Boolean network modeling of the changes in the dynamics of intracellular signaling pathways revealed NFKB activation by secretome of macrophages, leading to a decreased efficacy of Etanercept, suggesting NFKB inhibition as an alternative approach to inhibit cancer cell growth in the presence of macrophage crosstalk. CONCLUSION This study indicates that the effect of Etanercept may be influenced by residing macrophages in tumor microenvironment, and suggests a method to predict the effect of drugs in the presence of stromal cells to guide experimental designs in drug development.", "gemini": "BỐI CẢNH Sự tương tác giữa môi trường vi mô và các tế bào ung thư vú thường không được xem xét ở giai đoạn đầu của quá trình phát triển thuốc dẫn đến sự thất bại của nhiều loại thuốc ở các giai đoạn lâm sàng sau này. Etanercept là một chất ức chế TNF-alpha đã được nghiên cứu về tác dụng chống khối u tiềm tàng ở ung thư vú với kết quả trái ngược nhau. PHƯƠNG PHÁP Dữ liệu tiết ra trên dòng tế bào ung thư MDA-MB-231 đến từ các kho lưu trữ công cộng và được phân tích làm giàu gen. Vì các tế bào MDA-MB-231 tiết ra nồng độ cao Yếu tố kích thích tập đoàn bạch cầu hạt-bạch cầu đơn nhân, kích hoạt đại thực bào để thúc đẩy sự phát triển của khối u, nên tác dụng của việc nuôi cấy chung đại thực bào đối với hiệu quả chống ung thư của Etanercept trong ung thư vú đã được đánh giá bằng cách sử dụng mô hình mạng Boolean và các thí nghiệm trong ống nghiệm bao gồm xâm lấn, chu kỳ tế bào, Annexin PI và thử nghiệm khả năng sống dựa trên tetrazolium và hoạt động NFKB. KẾT QUẢ Hồ sơ tiết ra của các tế bào MDA-MB-231 tương tự như biểu hiện của các gen sau khi điều trị các tế bào ung thư vú bằng TNF-α. Theo đó, sự ức chế TNF-α bởi Etanercept làm giảm khả năng sống của tế bào MDA-MB-231, gây ra apoptosis và bắt giữ chu kỳ tế bào trong ống nghiệm và ức chế hoạt hóa NFKB. Tác dụng ức chế của Etanercept đối với khả năng sống của tế bào, sự tiến triển của chu kỳ tế bào, sự xâm lấn và gây apoptosis giảm sau khi nuôi cấy chung các tế bào ung thư với đại thực bào. Mô hình mạng Boolean về những thay đổi trong động lực của các con đường truyền tín hiệu trong tế bào cho thấy sự hoạt hóa NFKB bởi tiết ra của đại thực bào, dẫn đến hiệu quả giảm của Etanercept, gợi ý rằng ức chế NFKB là một phương pháp tiếp cận thay thế để ức chế sự phát triển của tế bào ung thư khi có sự tương tác của đại thực bào. KẾT LUẬN Nghiên cứu này chỉ ra rằng tác dụng của Etanercept có thể bị ảnh hưởng bởi các đại thực bào cư trú trong môi trường vi mô của khối u và đề xuất một phương pháp để dự đoán tác dụng của thuốc khi có các tế bào mô đệm để hướng dẫn thiết kế thử nghiệm trong phát triển thuốc."} +{"idx": 1880123, "en": "BACKGROUND Despite ample clinical evidence that gaining excess weight in pregnancy results in negative health outcomes for women and infants, more than half of women in Western industrialized nations gain in excess of national guidelines. The influence of socio-demographic factors and weight gain is well-established but not causal; the influence of psychological factors may explain some of this variation. METHODS This is the qualitative portion of an explanatory sequential mixed-methods study designed to identify predictive psychological factors of excess gestational weight gain (QUAN) and then explain the relevance of those factors (qual). For this portion of the study, we used a qualitative descriptive approach to elicit 39 pregnant women's perspectives of gestational weight gain, specifically inquiring about factors determined as relevant to excess gestational weight gain by our previous predictive study. Women were interviewed in the latter half of their third trimester. Data were analyzed using a combination of unconstrained deductive content analysis to describe the findings relevant to the predictive factors and a staged inductive content analytic approach to examine the data without a focus on the predictive factors. RESULTS Very few participants consistently made deliberate choices relevant to weight gain; most behaviour relevant to weight gain happened with in-the-moment decisions. These in-the-moment decisions were influenced by priorities, hunger, a consideration of the consequence of the decision, and accommodation of pregnancy-related discomfort. They were informed by the foundational information a woman had available to her, including previous experience and interactions with health care providers. The foundational information women used to make these decisions was often incomplete. While women were aware of the guidelines related to gestational weight gain, they consistently mis-applied them due to incorrect understanding of their own BMI. Only one woman was aware that weight gain was linked to maternal and infant health outcomes. CONCLUSIONS There is an important role for prenatal providers to provide the foundational information to positively influence in-the-moment decisions. Understanding how weight gain guidelines apply to one's own pre-pregnancy BMI and comprehending the well-established link between gestational weight gain and health outcomes may help women prioritize healthy weight gain amongst many competing factors.", "gemini": "BỐI CẢNH Mặc dù có nhiều bằng chứng lâm sàng cho thấy việc tăng cân quá mức trong thai kỳ dẫn đến hậu quả sức khỏe tiêu cực cho phụ nữ và trẻ sơ sinh, nhưng hơn một nửa phụ nữ ở các quốc gia công nghiệp phương Tây tăng cân vượt quá hướng dẫn quốc gia. Ảnh hưởng của các yếu tố xã hội-nhân khẩu học và tăng cân đã được xác lập rõ ràng nhưng không phải là nguyên nhân; ảnh hưởng của các yếu tố tâm lý có thể giải thích một số biến thể này. PHƯƠNG PHÁP Đây là phần định tính của nghiên cứu phương pháp hỗn hợp tuần tự giải thích được thiết kế để xác định các yếu tố tâm lý tiên đoán tăng cân quá mức trong thai kỳ (QUAN) và sau đó giải thích sự liên quan của các yếu tố đó (chất lượng). Đối với phần nghiên cứu này, chúng tôi đã sử dụng phương pháp mô tả định tính để thu thập quan điểm của 39 phụ nữ mang thai về việc tăng cân trong thai kỳ, đặc biệt là tìm hiểu về các yếu tố được xác định có liên quan đến việc tăng cân quá mức trong thai kỳ theo nghiên cứu dự đoán trước đây của chúng tôi. Phụ nữ được phỏng vấn vào nửa cuối tam cá nguyệt thứ ba của họ. Dữ liệu được phân tích bằng cách kết hợp phân tích nội dung diễn dịch không ràng buộc để mô tả các phát hiện có liên quan đến các yếu tố dự đoán và phương pháp phân tích nội dung quy nạp theo giai đoạn để kiểm tra dữ liệu mà không tập trung vào các yếu tố dự đoán. KẾT QUẢ Rất ít người tham gia liên tục đưa ra những lựa chọn có chủ ý liên quan đến việc tăng cân; hầu hết các hành vi có liên quan đến việc tăng cân đều xảy ra với các quyết định trong thời điểm đó. Những quyết định trong thời điểm đó chịu ảnh hưởng của các ưu tiên, cơn đói, cân nhắc hậu quả của quyết định và thích nghi với sự khó chịu liên quan đến thai kỳ. Họ được thông báo bởi thông tin cơ bản mà người phụ nữ có sẵn cho mình, bao gồm kinh nghiệm trước đây và tương tác với các nhà cung cấp dịch vụ chăm sóc sức khỏe. Thông tin cơ bản mà phụ nữ sử dụng để đưa ra những quyết định này thường không đầy đủ. Mặc dù phụ nữ biết về các hướng dẫn liên quan đến việc tăng cân trong thai kỳ, nhưng họ vẫn liên tục áp dụng sai do hiểu không đúng về BMI của chính mình. Chỉ có một phụ nữ biết rằng tăng cân có liên quan đến sức khỏe của mẹ và trẻ sơ sinh. KẾT LUẬN Các nhà cung cấp dịch vụ trước sinh có vai trò quan trọng trong việc cung cấp thông tin cơ bản để ảnh hưởng tích cực đến các quyết định trong thời điểm đó. Hiểu cách các hướng dẫn tăng cân áp dụng cho BMI trước khi mang thai của chính mình và hiểu mối liên hệ chặt chẽ giữa việc tăng cân trong thai kỳ và kết quả sức khỏe có thể giúp phụ nữ ưu tiên tăng cân lành mạnh trong số nhiều yếu tố cạnh tranh."} +{"idx": 1880124, "en": "BACKGROUND In this paper, we examine the ecological factors associated with death rates from suicide in the United States in 1999 and 2017, a period when suicide mortality increased in the United States. We focus on Non-Hispanic Whites, who experienced the largest increase in suicide mortality. We ask whether variation in suicide mortality among commuting zones can be explained by measures of the social and economic environment and access to lethal means used to kill oneself in one's area of residence. METHODS We use vital statistics data on deaths and Census Bureau population estimates and define area of residence as one of 704 commuting zones. We estimate separate models for men and women at ages 20-64 and 65 and above. We measure economic environment by percent of the workforce in manufacturing and the unemployment rate and social environment by marital status, educational attainment, and religious participation. We use gun sellers and opioid prescriptions as measures of access to lethal means. RESULTS We find that the strongest contextual predictors of higher suicide mortality are lower rates of manufacturing employment and higher rates of opiate prescriptions for all age/sex groups, increased gun accessibility for men, and religious participation for older people. CONCLUSIONS Socioeconomic characteristic and access to lethal means explain much of the variation in suicide mortality rates across commuting zones, but do not account for the pervasive national-level increase in suicide mortality between 1999 and 2017.", "gemini": "BỐI CẢNH Trong bài báo này, chúng tôi xem xét các yếu tố sinh thái liên quan đến tỷ lệ tử vong do tự tử ở Hoa Kỳ vào năm 1999 và 2017, thời kỳ tỷ lệ tử vong do tự tử tăng ở Hoa Kỳ. Chúng tôi tập trung vào người da trắng không phải gốc Tây Ban Nha, những người có tỷ lệ tử vong do tự tử tăng cao nhất. Chúng tôi đặt câu hỏi liệu sự thay đổi về tỷ lệ tử vong do tự tử giữa các khu vực đi lại có thể được giải thích bằng các biện pháp về môi trường xã hội và kinh tế và khả năng tiếp cận các phương tiện gây chết người được sử dụng để tự sát trong khu vực cư trú của một người hay không. PHƯƠNG PHÁP Chúng tôi sử dụng dữ liệu thống kê quan trọng về tử vong và ước tính dân số của Cục điều tra dân số và xác định khu vực cư trú là một trong 704 khu vực đi lại. Chúng tôi ước tính các mô hình riêng biệt cho nam và nữ ở độ tuổi 20-64 và 65 trở lên. Chúng tôi đo lường môi trường kinh tế theo tỷ lệ phần trăm lực lượng lao động trong ngành sản xuất và tỷ lệ thất nghiệp và môi trường xã hội theo tình trạng hôn nhân, trình độ học vấn và sự tham gia tôn giáo. Chúng tôi sử dụng người bán súng và đơn thuốc opioid làm biện pháp tiếp cận các phương tiện gây chết người. KẾT QUẢ Chúng tôi thấy rằng các yếu tố dự báo theo ngữ cảnh mạnh nhất về tỷ lệ tử vong do tự tử cao hơn là tỷ lệ lao động sản xuất thấp hơn và tỷ lệ kê đơn thuốc phiện cao hơn đối với tất cả các nhóm tuổi/giới tính, khả năng tiếp cận súng tăng đối với nam giới và sự tham gia tôn giáo đối với người cao tuổi. KẾT LUẬN Đặc điểm kinh tế xã hội và khả năng tiếp cận các phương tiện gây chết người giải thích phần lớn sự thay đổi về tỷ lệ tử vong do tự tử trên các khu vực đi lại, nhưng không tính đến sự gia tăng toàn quốc về tỷ lệ tử vong do tự tử trong giai đoạn 1999-2017."} +{"idx": 1880125, "en": "BACKGROUND Congenital cataract (CC) is a significant cause of lifelong visual loss, and its genetic diagnosis is challenging due to marked genetic heterogeneity. The purpose of this article is to report the genetic findings in sporadic and familial CC patients. METHODS Patients (n = 53) who were clinically diagnosed with CC and their parents were recruited. Blood samples were collected in our hospital. Mutations were detected by panel-based next-generation DNA sequencing (NGS) targeting 792 genes frequently involved in common inherited eye diseases. RESULTS We identified variants in 10/37 cases (27.02%) of sporadic CC and 14/16 cases (87.5%) of familial CC, which indicated a significant difference (P = 0.000). Of the 13 variants identified in sporadic cases, nine were previously reported mutations, and three were novel mutations, including one de novo mutation (CRYBB2 c.487C > T). The most frequent variants in our cohort were in crystallins and cytoskeletal genes (5/27, 18.52%), followed by proteins associated with X-linked syndromic conditions (14.81%) and transcriptional factors (11.11%). Additional information on the possibility of complications with inherited ocular or systemic diseases other than CC was provided in 17/27 (62.96%) variants. CONCLUSIONS These results contribute to expanding the mutation spectrum and frequency of genes responsible for CC. Targeted NGS in CC provided significant diagnostic information and enabled more accurate genetic counselling. This study reports the different distributions of mutation genes in familial and sporadic CC cases.", "gemini": "BỐI CẢNH Đục thủy tinh thể bẩm sinh (CC) là một nguyên nhân đáng kể gây mất thị lực suốt đời và chẩn đoán di truyền của nó rất khó khăn do tính không đồng nhất về di truyền. Mục đích của bài viết này là báo cáo các phát hiện di truyền ở những bệnh nhân CC lẻ tẻ và gia đình. PHƯƠNG PHÁP Bệnh nhân (n = 53) được chẩn đoán lâm sàng mắc CC và cha mẹ của họ đã được tuyển dụng. Các mẫu máu được thu thập tại bệnh viện của chúng tôi. Các đột biến được phát hiện bằng giải trình tự DNA thế hệ tiếp theo dựa trên bảng nhắm mục tiêu 792 gen thường liên quan đến các bệnh về mắt di truyền phổ biến. KẾT QUẢ Chúng tôi đã xác định các biến thể trong 10/37 trường hợp (27,02%) CC lẻ tẻ và 14/16 trường hợp (87,5%) CC gia đình, cho thấy sự khác biệt đáng kể (P = 0,000). Trong số 13 biến thể được xác định ở các trường hợp lẻ tẻ, chín biến thể là đột biến đã được báo cáo trước đó và ba biến thể là đột biến mới, bao gồm một đột biến de novo (CRYBB2 c.487C> T). Các biến thể thường gặp nhất trong nhóm của chúng tôi là ở các gen về thể thủy tinh và bộ khung tế bào (5/27, 18,52%), tiếp theo là các protein liên quan đến các tình trạng hội chứng liên quan đến X (14,81%) và các yếu tố phiên mã (11,11%). Thông tin bổ sung về khả năng xảy ra biến chứng với các bệnh về mắt hoặc toàn thân di truyền khác ngoài CC đã được cung cấp trong 17/27 (62,96%) biến thể. KẾT LUẬN Những kết quả này góp phần mở rộng phổ đột biến và tần số gen chịu trách nhiệm cho CC. NGS được nhắm mục tiêu trong CC cung cấp thông tin chẩn đoán quan trọng và cho phép tư vấn di truyền chính xác hơn. Nghiên cứu này báo cáo sự phân bố khác nhau của các gen đột biến trong các trường hợp CC gia đình và lẻ tẻ."} +{"idx": 1880126, "en": "BACKGROUND We aimed to identify which part of the patients with metastatic renal cell carcinoma (mRCC) is not suitable for cytoreductive nephrectomy (CN). METHODS The data of mRCC patients was acquired from the Surveillance, Epidemiology, and End Results (SEER) database. Multivariate cox regression analysis and nomogram were performed for selecting factors independently associated with survival. Propensity score matching (PSM) was applied to reduce potential bias when comparing survival of mRCC patients treated by CN or non-surgery (NS). The survival analysis of subgroups was estimated by the Kaplan-Meier method and compared by log-rank testing. The summary of subgroup analysis was showed by forest plots. RESULTS The records of 21,411 patients with mRCC were obtained from the SEER database. After screening, a total of 6532 patients were included for further analysis, of which 6043 underwent CN and 489 underwent NS. Age, T stage, N stage and tumor size were involved in subgroup analysis by PSM according to the result of multivariate cox regression analysis and clinical experience. Survival benefit was not found in T4 stage patients. Further analysis showed that T4&N1 and T4&age ≥ 76 yr subgroups could not obtain survival benefit from CN. CONCLUSION CN should not be performed in T4 stage mRCC patients who were in status of N1 stage or older than 76 years, because surgery cannot take significant survival benefit for them.", "gemini": "BỐI CẢNH Chúng tôi đặt mục tiêu xác định nhóm bệnh nhân ung thư biểu mô tế bào thận di căn (mRCC) nào không phù hợp với phẫu thuật cắt bỏ thận cắt giảm khối u (CN). PHƯƠNG PHÁP Chúng tôi thu thập dữ liệu của bệnh nhân mRCC từ cơ sở dữ liệu Giám sát, dịch tễ học và kết quả cuối cùng (SEER). Phân tích hồi quy cox đa biến và biểu đồ chuẩn được thực hiện để lựa chọn các yếu tố có liên quan độc lập với tỷ lệ sống sót. Ghép điểm khuynh hướng (PSM) được áp dụng để giảm độ lệch tiềm ẩn khi so sánh tỷ lệ sống sót của bệnh nhân mRCC được điều trị bằng CN hoặc không phẫu thuật (NS). Phân tích sống sót của các nhóm phụ được ước tính bằng phương pháp Kaplan-Meier và so sánh bằng kiểm định log-rank. Tóm tắt phân tích nhóm phụ được thể hiện bằng biểu đồ rừng. KẾT QUẢ Hồ sơ bệnh án của 21.411 bệnh nhân mRCC được lấy từ cơ sở dữ liệu SEER. Sau khi sàng lọc, tổng cộng 6532 bệnh nhân được đưa vào để phân tích sâu hơn, trong đó 6043 bệnh nhân được phẫu thuật CN và 489 bệnh nhân được phẫu thuật NS. Tuổi, giai đoạn T, giai đoạn N và kích thước khối u được đưa vào phân tích nhóm phụ bằng PSM theo kết quả phân tích hồi quy cox đa biến và kinh nghiệm lâm sàng. Lợi ích về tỷ lệ sống sót không được tìm thấy ở những bệnh nhân giai đoạn T4. Phân tích sâu hơn cho thấy các nhóm phụ T4 & N1 và T4 & tuổi ≥ 76 tuổi không thể có được lợi ích về tỷ lệ sống sót từ CN. KẾT LUẬN Không nên thực hiện CN ở những bệnh nhân mRCC giai đoạn T4 ở giai đoạn N1 hoặc trên 76 tuổi, vì phẫu thuật không mang lại lợi ích đáng kể về tỷ lệ sống sót cho họ."} +{"idx": 1880127, "en": "BACKGROUND Studies on anxiety and depression in unruptured intracranial aneurysm (UIA) patients after treatment via endovascular intervention are rare and controversial. We aimed to explore the prevalence of anxiety and depression among Chinese patients with UIAs treated by endovascular intervention and to identify which factors contribute to the development of these symptoms. METHODS We performed a cross-sectional study on anxiety and depression in patients who underwent endovascular treatment for UIAs using the Hospital Anxiety and Depression Scale (HADS). The demographic, clinical and radiological data for all patients were retrospectively collected from the aneurysm database and medical records. Moreover, we utilized data from a large sample of 200 UIA patients and multivariate logistic regression analysis to investigate the risk factors for anxiety and depression in these patients. Candidate variables with P values less than 0.20 in univariate analysis were included in the multivariate logistic regression analysis. RESULTS Two hundred patients returned completed questionnaires in this study. Of these 200 patients, 34 (17.0%) suffered from anxiety and 31 (15.5%) suffered from depression 30.67 ± 8.6 months after being discharged. The multivariate analysis results indicated that shorter sleep times were statistically significantly associated with depression (OR = 1.62, 95% CI: 1.14 ~ 2.29, P = 0.007, Adjusted P = 0.02). . CONCLUSION The prevalences of anxiety and depression in UIA patients treated by endovascular intervention were 17.0 and 15.5%, respectively. Shorter sleep times were significantly associated with depression. Our findings provide evidence for the clinical and psychological management of these patients.", "gemini": "BỐI CẢNH Các nghiên cứu về lo âu và trầm cảm ở những bệnh nhân phình động mạch nội sọ chưa vỡ (UIA) sau khi điều trị bằng can thiệp nội mạch rất hiếm và gây nhiều tranh cãi. Chúng tôi nhằm mục đích tìm hiểu tỷ lệ lo âu và trầm cảm ở những bệnh nhân UIA người Trung Quốc được điều trị bằng can thiệp nội mạch và xác định những yếu tố nào góp phần vào sự phát triển của các triệu chứng này. PHƯƠNG PHÁP Chúng tôi đã thực hiện một nghiên cứu cắt ngang về lo âu và trầm cảm ở những bệnh nhân được điều trị nội mạch đối với UIA bằng Thang lo âu và trầm cảm bệnh viện (HADS). Dữ liệu nhân khẩu học, lâm sàng và hình ảnh của tất cả bệnh nhân được thu thập hồi cứu từ cơ sở dữ liệu phình động mạch và hồ sơ bệnh án. Ngoài ra, chúng tôi đã sử dụng dữ liệu từ một mẫu lớn gồm 200 bệnh nhân UIA và phân tích hồi quy logistic đa biến để điều tra các yếu tố nguy cơ gây lo âu và trầm cảm ở những bệnh nhân này. Các biến ứng viên có giá trị P nhỏ hơn 0,20 trong phân tích đơn biến được đưa vào phân tích hồi quy logistic đa biến. KẾT QUẢ Hai trăm bệnh nhân đã trả lời đầy đủ bảng câu hỏi trong nghiên cứu này. Trong số 200 bệnh nhân này, 34 (17,0%) bị lo âu và 31 (15,5%) bị trầm cảm 30,67 ± 8,6 tháng sau khi xuất viện. Kết quả phân tích đa biến chỉ ra rằng thời gian ngủ ngắn hơn có liên quan đáng kể về mặt thống kê với chứng trầm cảm (OR = 1,62, KTCB 95%: 1,14 ~ 2,29, P = 0,007, P đã điều chỉnh = 0,02). KẾT LUẬN Tỷ lệ lo âu và trầm cảm ở những bệnh nhân UIA được điều trị bằng can thiệp nội mạch lần lượt là 17,0 và 15,5%. Thời gian ngủ ngắn hơn có liên quan đáng kể với chứng trầm cảm. Những phát hiện của chúng tôi cung cấp bằng chứng cho việc quản lý lâm sàng và tâm lý cho những bệnh nhân này."} +{"idx": 1880128, "en": "BACKGROUND As a low-middle income country, South Africa has seen an upsurge in the double burden of malnutrition (DBM). Owing to the rising costs of obesity on healthcare in South Africa, the National Treasury implemented a fiscal policy for the taxation of SSBs, known as the Health Promotion Levy, in line with the WHO recommendation. Potential negative impacts of the policy on the sugar cane industry and economic and rural development have been voiced by different sectors. By including a subsection in the SSBs fiscal policy and aligning the goals with existing policies, government could have made provisions for sugar cane farms to substitute crops with alternatives, including nutritional alternatives where possible, while supporting existing small-scale farms to produce nutrient-dense, local and culturally acceptable crops. Thus, the purpose of the study is to understand the perceptions of the various stakeholders on combining nutrition-sensitive agricultural interventions with the taxation on sugar-sweetened beverages (SSBs) to improve overall health and nutrition in South Africa. METHODS Semi-structured, in-depth interviews were conducted with each participant. The interviews were audio-recorded, transcribed intelligent verbatim, and cross-checked against the audio-recordings by the principal researcher. ATLAS.ti 8 software was used to navigate the data and assist with thematic analysis. RESULTS Perceptions of combining SSB taxation with agricultural policies to improve food and nutrition security were positive. The participants found it to be an innovative idea in theory but questioned the feasibility of combining policies. Participants highlighted education as an essential element for successfully changing behaviour to ensure a positive impact of the combined policy approach. Participants believed that before government could scale up nutrition-sensitive agricultural interventions, basic services and government functions would first need to run optimally. CONCLUSION Overall, perceptions with regard to combining the taxation on SSBs with nutrition-sensitive agricultural policies to improve overall health and nutrition in South Africa were positive. Although participants questioned the feasibility of combining these policies, it was viewed as a way to combat alleged collateral damage linked to the tax, with a specific focus on developing small-scale farmers. More research into these combined policy approaches in a South African context is required.", "gemini": "BỐI CẢNH Là một quốc gia có thu nhập trung bình thấp, Nam Phi đã chứng kiến sự gia tăng gánh nặng kép của tình trạng suy dinh dưỡng (DBM). Do chi phí ngày càng tăng của bệnh béo phì đối với chăm sóc sức khỏe ở Nam Phi, Bộ Tài chính đã thực hiện chính sách tài khóa đánh thuế đồ uống có đường (SSB), được gọi là Thuế thúc đẩy sức khỏe, theo khuyến nghị của WHO. Các tác động tiêu cực tiềm ẩn của chính sách đối với ngành mía đường và phát triển kinh tế và nông thôn đã được nhiều ngành khác nhau lên tiếng. Bằng cách đưa vào một tiểu mục trong chính sách tài khóa đối với SSB và liên kết các mục tiêu với các chính sách hiện hành, chính phủ có thể đưa ra các điều khoản cho các trang trại mía đường thay thế cây trồng bằng các loại cây thay thế, bao gồm các loại cây thay thế dinh dưỡng khi có thể, đồng thời hỗ trợ các trang trại quy mô nhỏ hiện có để sản xuất các loại cây trồng giàu dinh dưỡng, địa phương và phù hợp với văn hóa. Do đó, mục đích của nghiên cứu này là để hiểu nhận thức của các bên liên quan khác nhau về việc kết hợp các biện pháp can thiệp nông nghiệp nhạy cảm với dinh dưỡng với việc đánh thuế đồ uống có đường (SSB) để cải thiện sức khỏe và dinh dưỡng nói chung ở Nam Phi. PHƯƠNG PHÁP Các cuộc phỏng vấn chuyên sâu, có cấu trúc bán cấu trúc đã được thực hiện với từng người tham gia. Các cuộc phỏng vấn được ghi âm, phiên âm chính xác và kiểm tra chéo với các bản ghi âm bằng nhà nghiên cứu chính. Phần mềm ATLAS.ti 8 được sử dụng để điều hướng dữ liệu và hỗ trợ phân tích chủ đề. KẾT QUẢ Nhận thức về việc kết hợp đánh thuế SSB với các chính sách nông nghiệp để cải thiện an ninh lương thực và dinh dưỡng là tích cực. Những người tham gia thấy đây là một ý tưởng sáng tạo về mặt lý thuyết nhưng đặt câu hỏi về tính khả thi của việc kết hợp các chính sách. Những người tham gia nhấn mạnh giáo dục là một yếu tố thiết yếu để thay đổi hành vi thành công nhằm đảm bảo tác động tích cực của cách tiếp cận chính sách kết hợp. Những người tham gia tin rằng trước khi chính phủ có thể mở rộng các biện pháp can thiệp nông nghiệp nhạy cảm với dinh dưỡng, các dịch vụ cơ bản và chức năng của chính phủ trước tiên cần phải hoạt động tối ưu. KẾT LUẬN Nhìn chung, nhận thức về việc kết hợp đánh thuế SSB với các chính sách nông nghiệp nhạy cảm với dinh dưỡng để cải thiện sức khỏe và dinh dưỡng nói chung ở Nam Phi là tích cực. Mặc dù những người tham gia đặt câu hỏi về tính khả thi của việc kết hợp các chính sách này, nhưng nó được coi là một cách để chống lại thiệt hại tài sản thế chấp bị cáo buộc liên quan đến thuế, với trọng tâm cụ thể là phát triển các trang trại quy mô nhỏ. Cần có thêm nghiên cứu về các cách tiếp cận chính sách kết hợp này trong bối cảnh Nam Phi."} +{"idx": 1880129, "en": "BACKGROUND It is unknown whether microangiopathic ischemic strokes outside the visual pathway go along with subclinical changes of the retinal structure or the visual system. The objectives of this prospective non-interventional case series were to investigate if spectral-domain optical coherence tomography (SD-OCT) or multifocal visual evoked potentials (mfVEPs) can detect structural retinal changes or functional impairment of the visual system in patients with microangiopathic ischemic stroke. METHODS We used SD-OCT to cross-sectionally analyze the retinal morphology of 15 patients with microangiopathic ischemic stroke according to the Trial of Org 10172 in Acute Stroke Treatment (TOAST) classification not affecting the visual pathway. We employed semi-automated segmentation of macular volume scans to analyze the thickness of the macular retinal layers and peripapillary ring scans to investigate the retinal morphology in comparison to a control group without stroke. Visual function was assessed by the mfVEP technique in 13 microangiopathic ischemic stroke patients. RESULTS First peak latency of mfVEPs was significantly delayed in the microangiopathic ischemic stroke group compared to the control patients. Neither the retinal layers nor the mfVEPs' amplitude differed between the microangiopathic ischemic stroke patients and the control group. CONCLUSIONS In conclusion, microangiopathic ischemic stroke patients presented a delayed first peak latency in mfVEPs as a sign of subclinical functional impairment of the visual pathway. However, our case series suggests no influence on retinal structure resulting from microangiopathic ischemic stroke outside the visual system. Larger and longitudinal studies are needed to confirm these mfVEP findings.", "gemini": "TÓM TẮT Không biết liệu các đột quỵ thiếu máu cục bộ vi mạch bên ngoài đường dẫn truyền thị giác có đi kèm với những thay đổi cận lâm sàng về cấu trúc võng mạc hoặc hệ thống thị giác hay không. Mục tiêu của loạt ca bệnh không can thiệp có triển vọng này là nghiên cứu xem chụp cắt lớp quang học miền phổ (SD-OCT) hoặc điện thế thị giác gợi đa tiêu (mfVEP) có thể phát hiện những thay đổi cấu trúc võng mạc hoặc suy giảm chức năng của hệ thống thị giác ở những bệnh nhân bị đột quỵ thiếu máu cục bộ vi mạch hay không. PHƯƠNG PHÁP Chúng tôi đã sử dụng SD-OCT để phân tích mặt cắt ngang hình thái võng mạc của 15 bệnh nhân bị đột quỵ thiếu máu cục bộ vi mạch theo phân loại Thử nghiệm Org 10172 trong Điều trị Đột quỵ Cấp tính (TOAST) không ảnh hưởng đến đường dẫn truyền thị giác. Chúng tôi sử dụng phân đoạn bán tự động các lần quét thể tích hoàng điểm để phân tích độ dày của các lớp võng mạc hoàng điểm và các lần quét vòng quanh đĩa thị để nghiên cứu hình thái võng mạc so với nhóm đối chứng không bị đột quỵ. Chức năng thị giác được đánh giá bằng kỹ thuật mfVEP ở 13 bệnh nhân đột quỵ thiếu máu cục bộ vi mạch. KẾT QUẢ Độ trễ đỉnh đầu tiên của mfVEP bị chậm lại đáng kể ở nhóm đột quỵ thiếu máu cục bộ vi mạch so với bệnh nhân đối chứng. Độ dày các lớp võng mạc cũng như biên độ mfVEP không khác nhau giữa bệnh nhân đột quỵ thiếu máu cục bộ vi mạch và nhóm đối chứng. KẾT LUẬN Tóm lại, bệnh nhân đột quỵ thiếu máu cục bộ vi mạch biểu hiện độ trễ đỉnh đầu tiên trên mfVEP như một dấu hiệu của suy giảm chức năng cận lâm sàng của đường dẫn truyền thị giác. Tuy nhiên, loạt ca bệnh của chúng tôi cho thấy không có ảnh hưởng đến cấu trúc võng mạc do đột quỵ thiếu máu cục bộ vi mạch bên ngoài hệ thống thị giác. Cần có các nghiên cứu lớn hơn và dài hạn hơn để xác nhận các phát hiện mfVEP này."} +{"idx": 1880130, "en": "BACKGROUND Kidney disease and dialysis significantly impact cognitive function across the age spectrum. Cognitive training (CT) and/or exercise training (ET) are promising approaches to preserve cognitive function among community-dwelling older adults, but have not been tested for cognition preservation in hemodialysis patients of all ages. In this manuscript, we summarize the protocol for the Interventions Made to Preserve Cognitive Function Trial (IMPCT). METHODS We will perform a 2 × 2 factorial randomized controlled trial (RCT) of eligible adult (≥18 years) hemodialysis initiates (n = 200) to test whether intradialytic CT (brain games on a tablet PC), ET (foot peddlers) and combined CT + ET while undergoing hemodialysis preserves executive function compared to standard of care (SC). Participants will engage in the interventions to which they are randomized for 6 months. The primary objective is to compare, among interventions, the 3-month change in executive function measured using the Trail Making Test A (TMTA) and B (TMTB); specifically, executive function is calculated as TMTB-TMTA to account for psychomotor speed. This primary outcome was selected based on findings from our pilot study. The secondary objectives are to compare the risk of secondary cognitive outcomes, ESKD-specific clinical outcomes, and patient-centered outcomes at 3-months and 6-months. All data collection and interventions are conducted in the dialysis center. DISCUSSION We hypothesize that receiving intradialytic CT or ET will better preserve executive function than SC but receiving combined CT + ET, will be the most effective intervention. The current trial will be an important step in understanding how intradialytic interventions might preserve cognitive health. TRIAL REGISTRATION Clinicaltrials.Gov (Date: 8/6/18): # NCT03616535 . Protocol Version: 10 (April 2020). FUNDING NIDDK R01DK114074.", "gemini": "TÓM TẮT Bệnh thận và chạy thận nhân tạo ảnh hưởng đáng kể đến chức năng nhận thức trong suốt phổ tuổi. Huấn luyện nhận thức (CT) và/hoặc huấn luyện thể dục (ET) là những phương pháp đầy hứa hẹn để bảo tồn chức năng nhận thức ở những người lớn tuổi sống trong cộng đồng, nhưng chưa được thử nghiệm để bảo tồn nhận thức ở những bệnh nhân chạy thận nhân tạo ở mọi lứa tuổi. Trong bản thảo này, chúng tôi tóm tắt giao thức cho Thử nghiệm can thiệp để bảo tồn chức năng nhận thức (IMPCT). PHƯƠNG PHÁP Chúng tôi sẽ thực hiện một thử nghiệm đối chứng ngẫu nhiên theo giai thừa 2 × 2 (RCT) đối với những người đủ điều kiện tham gia chạy thận nhân tạo (≥18 tuổi) (n = 200) để kiểm tra xem CT trong quá trình chạy thận nhân tạo (trò chơi trí não trên máy tính bảng), ET (xe đạp chân) và kết hợp CT + ET trong khi chạy thận nhân tạo có bảo tồn chức năng điều hành so với tiêu chuẩn chăm sóc (SC) không. Những người tham gia sẽ tham gia vào các can thiệp mà họ được phân ngẫu nhiên trong 6 tháng. Mục tiêu chính là so sánh, giữa các can thiệp, sự thay đổi chức năng điều hành sau 3 tháng được đo bằng Thử nghiệm Trail Making A (TMTA) và B (TMTB); cụ thể, chức năng điều hành được tính là TMTB-TMTA để tính đến tốc độ vận động tâm lý. Kết quả chính này được lựa chọn dựa trên các phát hiện từ nghiên cứu thí điểm của chúng tôi. Các mục tiêu phụ là so sánh nguy cơ kết quả nhận thức thứ cấp, kết quả lâm sàng đặc hiệu của ESKD và kết quả do bệnh nhân tập trung vào sau 3 tháng và 6 tháng. Tất cả các can thiệp và thu thập dữ liệu đều được thực hiện tại trung tâm chạy thận nhân tạo. THẢO LUẬN Chúng tôi đưa ra giả thuyết rằng việc nhận CT hoặc ET trong quá trình chạy thận nhân tạo sẽ bảo tồn chức năng điều hành tốt hơn so với SC nhưng việc nhận kết hợp CT + ET sẽ là can thiệp hiệu quả nhất. Thử nghiệm hiện tại sẽ là một bước quan trọng trong việc hiểu cách các can thiệp trong quá trình chạy thận nhân tạo có thể bảo tồn sức khỏe nhận thức. ĐĂNG KÝ THỬ NGHIỆM Clinicaltrials.Gov (Ngày: 8/6/18): # NCT03616535. Phiên bản giao thức: 10 (Tháng 4 năm 2020). TÀI TRỢ NIDDK R01DK114074."} +{"idx": 1880131, "en": "BACKGROUND Creatine (Cr), an amino acid derivative, is one of the most important sources of energy acting as both a spatial and temporal energy buffer through its phosphorylated analogue phosphocreatine (PCr) and creatine kinase (CK). Maternal Cr biosynthesis and metabolism seem to play an important role in pregnancy, as shown in preclinical and in healthy human pregnancy studies. Patients with Arginine:Glycine Amidino-Transferase deficiency (AGAT-d), due to the deficit of the first enzyme involved in Cr synthesis, are at a disadvantage due to their failure to synthesize Cr and their dependence on external intake, in contrast to normal subjects, where changes in Cr biosynthesis supply their needs. We report the outcomes of a pregnancy in an AGAT-d woman, and the challenge we faced in managing her treatment with oral Cr to ensure optimal conditions for her fetus. CASE PRESENTATION A 22-year-old AGAT-d woman referred to our Institute for the management of her first conception at 11 weeks of fetal gestational age. Sonographic monitoring at 20 w GA indicated a reduction of fetal growth, in particular of the head circumference that was below the 3rd centile. Biochemical monitoring of Cr in biological fluids of the mother revealed a decline of the Cr concentrations, in particular in the urine sample, requiring prompt correction of the Cr dose. At 35 weeks of gestation the patient delivered a male infant, heterozygous for GATM mutation, with normal brain Cr levels; at one year the baby achieved typical developmental milestones. CONCLUSIONS This rare pregnancy demonstrates that Cr levels in the blood and urine of the mother with AGAT-d decreased since the first months of gestation. The increase of the Cr daily dose administered to the mother seems to have produced beneficial effects also on the fetus.", "gemini": "TÓM TẮT Creatine (Cr), một dẫn xuất của axit amin, là một trong những nguồn năng lượng quan trọng nhất hoạt động như một chất đệm năng lượng về mặt không gian và thời gian thông qua chất tương tự phosphoryl hóa của nó, phosphocreatine (PCr) và creatine kinase (CK). Quá trình tổng hợp và chuyển hóa Cr của mẹ dường như đóng một vai trò quan trọng trong thai kỳ, như đã được chứng minh trong các nghiên cứu tiền lâm sàng và trong các nghiên cứu về thai kỳ khỏe mạnh của con người. Những bệnh nhân bị thiếu Arginine: Glycine Amidino-Transferase (AGAT-d), do thiếu hụt enzyme đầu tiên tham gia vào quá trình tổng hợp Cr, sẽ gặp bất lợi do không tổng hợp được Cr và phụ thuộc vào lượng hấp thụ từ bên ngoài, trái ngược với những đối tượng bình thường, khi những thay đổi trong quá trình tổng hợp Cr đáp ứng được nhu cầu của họ. Chúng tôi báo cáo kết quả của một thai kỳ ở một phụ nữ mắc AGAT-d và những thách thức mà chúng tôi phải đối mặt trong việc quản lý việc điều trị bằng Cr đường uống của cô ấy để đảm bảo điều kiện tối ưu cho thai nhi. TRÌNH BÀY CA BỆNH Một phụ nữ mắc AGAT-d 22 tuổi được giới thiệu đến Viện của chúng tôi để quản lý lần mang thai đầu tiên của cô ấy ở tuần thứ 11 của thai kỳ. Theo dõi siêu âm ở tuần thứ 20 cho thấy sự chậm phát triển của thai nhi, đặc biệt là chu vi vòng đầu dưới mức chuẩn thứ 3. Theo dõi sinh hóa Cr trong dịch sinh học của người mẹ cho thấy nồng độ Cr giảm, đặc biệt là trong mẫu nước tiểu, đòi hỏi phải nhanh chóng điều chỉnh liều Cr. Ở tuần thứ 35 của thai kỳ, bệnh nhân sinh một bé trai, dị hợp tử về đột biến GATM, với nồng độ Cr não bình thường; ở một tuổi, em bé đạt được các mốc phát triển điển hình. KẾT LUẬN Thai kỳ hiếm gặp này chứng minh rằng nồng độ Cr trong máu và nước tiểu của người mẹ mắc AGAT-d đã giảm kể từ những tháng đầu của thai kỳ. Việc tăng liều Cr hàng ngày cho người mẹ dường như cũng có tác dụng có lợi cho thai nhi."} +{"idx": 1880132, "en": "BACKGROUND Merely having the tools to end HIV is insufficient. Effectively ending the epidemic necessitates addressing barriers that impede engagement in biomedical and behavioral prevention and wide scale implementation and utilization of existing interventions. This qualitative study identifies suggestions for increasing access to, engagement in, and impact of HIV prevention among women living in cities in high HIV burden counties in the eastern US. METHODS Data analyzed for the current study were collected via a qualitative sub-study within the HIV Prevention Trials Network Study 064 (HPTN 064), a multisite observational cohort study designed to estimate HIV incidence among women residing in communities with elevated HIV prevalence who also reported personal or partner characteristics associated with increased risk of HIV acquisition. Focus group and interview participants in the qualitative sub-study (N = 288) were from four cities in the eastern US. RESULTS Thematic analyses revealed four themes describing women's most frequently stated ideas for improving prevention efforts: 1) Promote Multilevel Empowerment, 2) Create Engaging Program Content, 3) Build \"Market Demand\", and 4) Ensure Accessibility. We conducted additional analyses to identify contradictory patterns in the data, which revealed an additional three themes: 1) Address Structural Risk Factors, 2) Increase Engagement via Pleasure Promotion, 3) Expand Awareness of and Access to Prevention Resources. CONCLUSIONS Findings may be useful for enhancing women's engagement in and uptake of behavioral and biomedical HIV prevention resources, improving policy, and addressing multilevel risk factors. TRIAL REGISTRATION Clinicaltrials.gov: NCT00995176 , prospectively registered.", "gemini": "BỐI CẢNH Chỉ có các công cụ để chấm dứt HIV là chưa đủ. Để chấm dứt hiệu quả đại dịch, cần phải giải quyết các rào cản cản trở việc tham gia vào các biện pháp phòng ngừa hành vi và y sinh học, cũng như việc thực hiện và sử dụng rộng rãi các biện pháp can thiệp hiện có. Nghiên cứu định tính này xác định các đề xuất nhằm tăng cường khả năng tiếp cận, tham gia và tác động của công tác phòng ngừa HIV ở những phụ nữ sống tại các thành phố thuộc các quận có gánh nặng HIV cao ở miền đông Hoa Kỳ. PHƯƠNG PHÁP Dữ liệu được phân tích cho nghiên cứu hiện tại được thu thập thông qua một nghiên cứu phụ định tính trong Nghiên cứu 064 của Mạng lưới thử nghiệm phòng ngừa HIV (HPTN 064), một nghiên cứu theo dõi quan sát đa địa điểm được thiết kế để ước tính tỷ lệ mắc HIV ở những phụ nữ cư trú trong các cộng đồng có tỷ lệ mắc HIV cao, những người cũng báo cáo các đặc điểm cá nhân hoặc của bạn tình liên quan đến nguy cơ mắc HIV cao hơn. Những người tham gia phỏng vấn và nhóm tập trung trong nghiên cứu phụ định tính (N = 288) đến từ bốn thành phố ở miền đông Hoa Kỳ. KẾT QUẢ Phân tích theo chủ đề đã tiết lộ bốn chủ đề mô tả những ý tưởng được nêu ra thường xuyên nhất của phụ nữ về việc cải thiện các nỗ lực phòng ngừa: 1) Thúc đẩy trao quyền ở nhiều cấp độ, 2) Tạo nội dung chương trình hấp dẫn, 3) Xây dựng \"Nhu cầu thị trường\" và 4) Đảm bảo khả năng tiếp cận. Chúng tôi đã tiến hành các phân tích bổ sung để xác định các mô hình trái ngược trong dữ liệu, qua đó tiết lộ thêm ba chủ đề: 1) Giải quyết các yếu tố rủi ro về mặt cấu trúc, 2) Tăng cường sự tham gia thông qua việc thúc đẩy sự thích thú, 3) Mở rộng nhận thức và khả năng tiếp cận các nguồn lực phòng ngừa. KẾT LUẬN Các phát hiện có thể hữu ích để tăng cường sự tham gia của phụ nữ và tiếp nhận các nguồn lực phòng ngừa HIV hành vi và y sinh học, cải thiện chính sách và giải quyết các yếu tố rủi ro ở nhiều cấp độ. ĐĂNG KÝ THỬ NGHIỆM Clinicaltrials.gov: NCT00995176, đã đăng ký trước."} +{"idx": 1880133, "en": "BACKGROUND There are multiple obstacles encountered by immigrants attempting to engage hepatitis C virus (HCV) care and treatment. We evaluated the diversity and treatment outcomes of HCV-infected immigrants evaluated for Direct Acting Antiviral (DAA) therapy in Canada. METHODS The Canadian Network Undertaking against Hepatitis C (CANUHC) Cohort contains demographic information and DAA treatment information prospectively collected at 10 Canadian sites. Information on country of origin and race are collected. Characteristics and outcomes (sustained virological response; SVR) were compared by immigration status and race. RESULTS Between January 2016 and May 2018, 725 HCV-infected patients assessed for DAA therapy were enrolled in CANUHC (mean age: 52.66 ± 12.68 years); 65.66% male; 82.08% White, 5.28% Indigenous, 4.64% South East Asian, 4.64% East Indian, 3.36% Black). 18.48% were born outside of Canada. Mean age was similar [immigrants: 54.36 ± 13.95 years), Canadian-born: 52.27 ± 12.35 years); (p = 0.085)]. The overall baseline fibrosis score (in kPa measured by transient elastography) was similar among Canadian and foreign-born patients. Fibrosis score was not predicted by race or genotype. The proportion initiating DAA therapy was similar by immigrant status (56.72% vs 49.92%). SVR rates by intent-to-treat analysis were similar (immigrants-89.47%, Canadian-born-92.52%; p = 0.575). CONCLUSION A diverse immigrant population is engaging care in Canada, initiating HCV antiviral therapy in an equitable fashion and achieving SVR proportions similar to Canada-born patients. Our Canadian experience may be of value in informing HCV elimination efforts in economically developed regions.", "gemini": "BỐI CẢNH Những người nhập cư gặp phải nhiều trở ngại khi cố gắng tiếp cận dịch vụ chăm sóc và điều trị virus viêm gan C (HCV). Chúng tôi đã đánh giá sự đa dạng và kết quả điều trị của những người nhập cư bị nhiễm HCV được đánh giá để điều trị bằng Thuốc kháng vi-rút tác dụng trực tiếp (DAA) tại Canada. PHƯƠNG PHÁP Mạng lưới Canada chống lại Viêm gan C (CANUHC) bao gồm thông tin nhân khẩu học và thông tin điều trị bằng DAA được thu thập trước tại 10 địa điểm của Canada. Thông tin về quốc gia gốc và chủng tộc được thu thập. Các đặc điểm và kết quả (đáp ứng vi-rút bền vững; SVR) được so sánh theo tình trạng nhập cư và chủng tộc. KẾT QUẢ Từ tháng 1 năm 2016 đến tháng 5 năm 2018, 725 bệnh nhân nhiễm HCV được đánh giá để điều trị bằng DAA đã được ghi danh vào CANUHC (tuổi trung bình: 52,66 ± 12,68 tuổi); 65,66% là nam giới; 82,08% là người da trắng, 5,28% là người bản địa, 4,64% là người Đông Nam Á, 4,64% là người Ấn Độ, 3,36% là người da đen). 18,48% sinh ra bên ngoài Canada. Tuổi trung bình là tương tự [người nhập cư: 54,36 ± 13,95 tuổi), sinh ra ở Canada: 52,27 ± 12,35 tuổi); (p = 0,085)]. Điểm xơ gan cơ bản chung (tính bằng kPa được đo bằng phương pháp đàn hồi thoáng qua) là tương tự ở những bệnh nhân sinh ra ở Canada và ở nước ngoài. Điểm xơ gan không được dự đoán bởi chủng tộc hoặc kiểu gen. Tỷ lệ bắt đầu điều trị bằng DAA là tương tự theo tình trạng nhập cư (56,72% so với 49,92%). Tỷ lệ SVR theo phân tích ý định điều trị là tương tự (người nhập cư-89,47%, người sinh ra ở Canada-92,52%; p = 0,575). KẾT LUẬN Một nhóm dân nhập cư đa dạng đang tham gia chăm sóc tại Canada, bắt đầu điều trị bằng thuốc kháng vi-rút HCV theo cách công bằng và đạt tỷ lệ SVR tương tự như những bệnh nhân sinh ra ở Canada. Kinh nghiệm của Canada có thể có giá trị trong việc cung cấp thông tin cho các nỗ lực loại trừ HCV ở các khu vực phát triển kinh tế."} +{"idx": 1880134, "en": "BACKGROUND Tuberculosis (TB)-related stigma presents a major barrier to care of persons with TB through its impact on treatment initiation and retention in care. This is particularly challenging in settings with high prevalence of both TB and HIV where fear of HIV/AIDS can amplify stigma surrounding TB. The purpose of this study was to validate a TB stigma scale for use among persons presenting for outpatient HIV screening in the Umlazi township of South Africa and evaluate factors associated with TB-related stigma in this high HIV burden setting. METHODS In this cross-sectional study, we measured TB-related stigma in adults prior to HIV testing using a 12-item scale designed to assess experienced and felt TB-related stigma. RESULTS Among 848 adults, mean age was 32 years, 54% were male, and the median TB stigma score was 19 of 36 (interquartile range 15-23). We identified two factors in the stigma scale which had excellent reliability (Cronbach's alpha 0.85, 0.89). Persons with high TB stigma were more likely to be male (adjusted relative risk ratio [aRRR] 1.56, 95% confidence interval [CI] 1.11-2.28) and have accurate knowledge of TB transmission (aRRR 1.90, 95% CI 1.16-3.10) as compared to those with low stigma. Variables not significantly associated with stigma in the multivariate model included education, income, prior TB or HIV diagnoses, and depression. CONCLUSIONS Male sex and TB knowledge were associated with higher TB stigma in an outpatient HIV clinic in a South African township. Identifying risk factors associated with stigma will be important to guide stigma reduction interventions.", "gemini": "BỐI CẢNH Sự kỳ thị liên quan đến bệnh lao (TB) là rào cản chính đối với việc chăm sóc những người mắc bệnh lao thông qua tác động của nó đến việc bắt đầu điều trị và duy trì chăm sóc. Điều này đặc biệt khó khăn trong bối cảnh có tỷ lệ mắc cả bệnh lao và HIV cao, nơi nỗi sợ HIV/AIDS có thể khuếch đại sự kỳ thị xung quanh bệnh lao. Mục đích của nghiên cứu này là xác nhận thang đo kỳ thị bệnh lao để sử dụng cho những người đến khám sàng lọc HIV ngoại trú tại thị trấn Umlazi của Nam Phi và đánh giá các yếu tố liên quan đến kỳ thị liên quan đến bệnh lao trong bối cảnh gánh nặng HIV cao này. PHƯƠNG PHÁP Trong nghiên cứu cắt ngang này, chúng tôi đã đo lường sự kỳ thị liên quan đến bệnh lao ở những người trưởng thành trước khi xét nghiệm HIV bằng cách sử dụng thang đo 12 mục được thiết kế để đánh giá sự kỳ thị liên quan đến bệnh lao mà họ trải qua và cảm thấy. KẾT QUẢ Trong số 848 người trưởng thành, tuổi trung bình là 32, 54% là nam và điểm kỳ thị bệnh lao trung bình là 19 trên 36 (khoảng tứ phân vị 15-23). Chúng tôi đã xác định được hai yếu tố trong thang đo kỳ thị có độ tin cậy tuyệt vời (alpha Cronbach 0,85, 0,89). Những người có kỳ thị bệnh lao cao có nhiều khả năng là nam giới (tỷ lệ rủi ro tương đối đã điều chỉnh [aRRR] 1,56, khoảng tin cậy 95% [CI] 1,11-2,28) và có kiến thức chính xác về quá trình lây truyền bệnh lao (aRRR 1,90, 95% CI 1,16-3,10) so với những người có kỳ thị thấp. Các biến không liên quan đáng kể đến kỳ thị trong mô hình đa biến bao gồm trình độ học vấn, thu nhập, chẩn đoán lao hoặc HIV trước đó và trầm cảm. KẾT LUẬN Giới tính nam và kiến thức về bệnh lao có liên quan đến kỳ thị bệnh lao cao hơn tại một phòng khám HIV ngoại trú tại một thị trấn của Nam Phi. Việc xác định các yếu tố rủi ro liên quan đến kỳ thị sẽ rất quan trọng để hướng dẫn các biện pháp can thiệp giảm kỳ thị."} +{"idx": 1880135, "en": "BACKGROUND This article describes the analysis and interpretation of data relating to the presence of cadmium, lead, mercury and fluoride in human bone samples obtained from cadavers of patients dying of Chronic Kidney Disease of uncertain aetiology (CKDu) in a case-control study, which the authors believe to be the first in Sri Lanka. METHODS This is a case-control study comparing selected nephrotoxins levels in autopsy samples of bones from persons confirmed to have died of CKDu, and who had lived in a CKDu hotspot and controls with no history of abnormal kidney functions who had lived in areas having a low prevalence of CKDu. RESULTS The average age at death of the cases was 59.6 ± 15.1 (±SD), while that for controls was 58.0 ± 19.3 (±SD) years with no significant statistical difference. Calcium adjusted bone lead and bone fluoride levels were significantly higher among CKDu cases (n = 14) than those of controls (n = 33). Further, younger and older clusters of CKDu cases can be differentiated when the calcium adjusted lead content was considered. Younger patients reported higher lead content compared to the older group. Cadmium and mercury content did not show a remarkable difference among cases and controls. CONCLUSIONS Our results show a significant accumulation of lead in bone tissue in persons who have died of CKDu which indicates a higher chronic exposure of the CKDu victims, to lead. Fluoride content in the bones of cases is also significantly high. The results indicate that a gradual reduction of glomerular filtration rate (GFR) may have occurred with the chronic exposure to lead. At a certain point of the threshold of low GFR, excretion of fluoride may be impaired, since the main excretory pathway of fluoride is via the kidneys. Fluoride accumulation in blood in turn may exacerbate nephrotoxicity, triggering a synergistic cascade of events which may lead to a further deterioration in the GFR. The extremely high fluoride content detected suggests a cumulative effect in people exposed to comparatively higher fluoride levels, most probably from drinking water.", "gemini": "BỐI CẢNH Bài viết này mô tả quá trình phân tích và diễn giải dữ liệu liên quan đến sự hiện diện của cadmium, chì, thủy ngân và florua trong các mẫu xương người lấy từ tử thi của những bệnh nhân tử vong do Bệnh Thận Mạn có nguyên nhân không rõ (CKDu) trong một nghiên cứu ca chứng, mà các tác giả tin rằng là nghiên cứu đầu tiên tại Sri Lanka. PHƯƠNG PHÁP Đây là một nghiên cứu ca chứng so sánh nồng độ của các chất độc thận đã chọn trong các mẫu xương tử thi của những người được xác nhận đã tử vong do CKDu và đã sống ở điểm nóng CKDu và những người đối chứng không có tiền sử chức năng thận bất thường đã sống ở những khu vực có tỷ lệ mắc CKDu thấp. KẾT QUẢ Độ tuổi trung bình khi tử vong của các trường hợp là 59,6 ± 15,1 (±SD), trong khi đó của nhóm đối chứng là 58,0 ± 19,3 (±SD) năm không có sự khác biệt thống kê đáng kể. Nồng độ chì trong xương và florua trong xương điều chỉnh theo canxi cao hơn đáng kể ở những trường hợp mắc CKDu (n = 14) so với nhóm đối chứng (n = 33). Hơn nữa, có thể phân biệt được các nhóm trường hợp mắc CKDu trẻ tuổi và lớn tuổi hơn khi xét đến hàm lượng chì điều chỉnh theo canxi. Những bệnh nhân trẻ tuổi có hàm lượng chì cao hơn so với nhóm lớn tuổi. Hàm lượng cadmium và thủy ngân không có sự khác biệt đáng kể giữa các trường hợp và nhóm đối chứng. KẾT LUẬN Kết quả của chúng tôi cho thấy sự tích tụ đáng kể chì trong mô xương ở những người đã tử vong do CKDu, điều này chỉ ra rằng các nạn nhân của CKDu bị phơi nhiễm mạn tính với chì ở mức độ cao hơn. Hàm lượng florua trong xương của các trường hợp cũng cao đáng kể. Kết quả cho thấy tốc độ lọc cầu thận (GFR) giảm dần có thể xảy ra khi tiếp xúc mạn tính với chì. Tại một thời điểm nhất định của ngưỡng GFR thấp, quá trình bài tiết florua có thể bị suy yếu, vì con đường bài tiết chính của florua là qua thận. Tích tụ florua trong máu lần lượt có thể làm trầm trọng thêm độc tính đối với thận, gây ra một loạt các sự kiện hiệp đồng có thể dẫn đến GFR xấu đi hơn nữa. Hàm lượng florua cực cao được phát hiện cho thấy tác dụng tích lũy ở những người tiếp xúc với nồng độ florua cao hơn so với mức bình thường, rất có thể là do uống nước."} +{"idx": 1880136, "en": "BACKGROUND Optic atrophy 1 (OPA1) gene mutations are associated with dominantly inherited optic neuropathy resulting in a progressive loss of visual acuity. Compound heterozygous or homozygous variants that lead to severe phenotypes, including Behr syndrome, have been reported rarely. CASE PRESENTATION Here, we present a 14-month-old boy with early onset optic atrophy, congenital cataracts, neuromuscular disorders, mental retardation, and developmental delay. Combined genetic testing, including whole exome sequencing (WES) and chromosomal microarray analysis, revealed a concurrent OPA1 variant (c.2189 T > C p.Leu730Ser) and de novo chromosome 3q deletion as pathogenic variants leading to the severe phenotype. CONCLUSIONS Our case is the first reporting a novel missense OPA1 variant co-occurring with a chromosomal microdeletion leading to a severe phenotype reminiscent of Behr syndrome. This expands the mutation spectrum of OPA1 and inheritance patterns of this disease.", "gemini": "BỐI CẢNH Các đột biến gen teo thị thần kinh 1 (OPA1) có liên quan đến bệnh thần kinh thị giác di truyền trội, dẫn đến mất dần thị lực. Các biến thể dị hợp tử kép hoặc đồng hợp tử dẫn đến kiểu hình nghiêm trọng, bao gồm hội chứng Behr, đã được báo cáo rất hiếm. TRIỂN BÀY TRƯỜNG HỢP Ở đây, chúng tôi trình bày một bé trai 14 tháng tuổi bị teo thị thần kinh sớm, đục thủy tinh thể bẩm sinh, rối loạn thần kinh cơ, chậm phát triển trí tuệ và chậm phát triển. Xét nghiệm di truyền kết hợp, bao gồm giải trình tự toàn bộ exome (WES) và phân tích mảng nhiễm sắc thể, đã phát hiện ra một biến thể OPA1 đồng thời (c.2189 T> C p.Leu730Ser) và mất đoạn nhiễm sắc thể 3q de novo là các biến thể gây bệnh dẫn đến kiểu hình nghiêm trọng. KẾT LUẬN Trường hợp của chúng tôi là trường hợp đầu tiên báo cáo một biến thể OPA1 vô nghĩa mới xảy ra đồng thời với vi mất đoạn nhiễm sắc thể dẫn đến kiểu hình nghiêm trọng gợi nhớ đến hội chứng Behr. Điều này mở rộng phổ đột biến của OPA1 và các kiểu di truyền của bệnh này."} +{"idx": 1880137, "en": "BACKGROUND Accurate anthropometric measurements are essential for assessing nutritional status, monitoring child growth, and informing clinical care. We aimed to improve height measurements of hospitalized pediatrics patients through implementation of gold standard measurement techniques. METHODS A quality improvement project implemented computerized training modules on anthropometry and standardized wooden boards for height measurements in a tertiary children's hospital. Heights were collected pre- and post-intervention on general pediatric inpatients under 5 years of age. Accuracy of height measurements was determined by analyzing the variance and by comparing to World Health Organization's defined biologically plausible height-for-age z-scores. Qualitative interviews assessed staff attitudes. RESULTS Ninety-six hospital staff completed the anthropometry training. Data were available on 632 children pre- and 933 post-intervention. Training did not increase the proportion of patients measured for height (78.6% pre-intervention vs. 75.8% post-intervention, p = 0.19). Post-intervention, wooden height boards were used to measure height of 34.8% patients, while tape measures and wingspan accounted for 42.0% and 3.5% of measurements, respectively. There was no improvement in the quality of height measurements based on plausibility (approximately 3% height-for-age z-scores measurements flagged out of range pre- and post-intervention), digit preference (13.4% of digits pre- and 12.3% post-intervention requiring reclassification), or dispersion of measurements (height-for-age z-scores standard deviation 1.9 pre- and post-intervention). Staff reported that using the wooden board was too labor consuming and cumbersome. CONCLUSIONS Our findings suggest that efforts to improve anthropometric measurements of hospitalized children have multiple obstacles, and further investigation of less cumbersome methods of measurements may be warranted.", "gemini": "BỐI CẢNH Các phép đo nhân trắc chính xác rất cần thiết để đánh giá tình trạng dinh dưỡng, theo dõi sự tăng trưởng của trẻ và cung cấp thông tin cho việc chăm sóc lâm sàng. Chúng tôi nhằm mục đích cải thiện các phép đo chiều cao của bệnh nhân nhi nhập viện thông qua việc triển khai các kỹ thuật đo lường tiêu chuẩn vàng. PHƯƠNG PHÁP Một dự án cải tiến chất lượng đã triển khai các mô-đun đào tạo bằng máy tính về nhân trắc học và các bảng gỗ tiêu chuẩn để đo chiều cao tại một bệnh viện nhi cấp ba. Chiều cao được thu thập trước và sau can thiệp ở bệnh nhân nội trú nhi khoa nói chung dưới 5 tuổi. Độ chính xác của các phép đo chiều cao được xác định bằng cách phân tích phương sai và so sánh với các điểm chuẩn z chiều cao theo tuổi được Tổ chức Y tế Thế giới định nghĩa là hợp lý về mặt sinh học. Các cuộc phỏng vấn định tính đánh giá thái độ của nhân viên. KẾT QUẢ Chín mươi sáu nhân viên bệnh viện đã hoàn thành khóa đào tạo nhân trắc học. Dữ liệu có sẵn trên 632 trẻ trước và 933 trẻ sau can thiệp. Đào tạo không làm tăng tỷ lệ bệnh nhân được đo chiều cao (78,6% trước can thiệp so với 75,8% sau can thiệp, p = 0,19). Sau can thiệp, các bảng đo chiều cao bằng gỗ đã được sử dụng để đo chiều cao của 34,8% bệnh nhân, trong khi thước dây và sải cánh chiếm lần lượt 42,0% và 3,5% các phép đo. Không có sự cải thiện nào về chất lượng các phép đo chiều cao dựa trên tính hợp lý (khoảng 3% các phép đo điểm chuẩn z chiều cao theo tuổi được đánh dấu ra khỏi phạm vi trước và sau can thiệp), sở thích chữ số (13,4% chữ số trước và 12,3% sau can thiệp yêu cầu phân loại lại), hoặc độ phân tán của các phép đo (độ lệch chuẩn điểm chuẩn z chiều cao theo tuổi 1,9 trước và sau can thiệp). Nhân viên báo cáo rằng việc sử dụng bảng gỗ quá tốn công sức và cồng kềnh. KẾT LUẬN Các phát hiện của chúng tôi cho thấy rằng những nỗ lực cải thiện các phép đo nhân trắc của trẻ em nhập viện gặp nhiều trở ngại và có thể cần phải điều tra thêm các phương pháp đo lường ít cồng kềnh hơn."} +{"idx": 1880138, "en": "BACKGROUND Keeping the patients well and fully informed about diagnosis, prognosis, and treatments is one of the patient's rights in any healthcare system. Although all healthcare providers have the same viewpoint about rendering the truth in treatment process, sometimes the truth is not told to the patients; that is why the healthcare staff tell \"white lie\" instead. This study aimed to explore the nurses' experience of white lies during patient care. METHODS This qualitative study was conducted from June to December 2018. Eighteen hospital nurses were recruited with maximum variation from ten state-run educational hospitals affiliated to Tehran University of Medical Sciences. Purposeful sampling was used and data were collected by semi-structured interviews that were continued until data saturation. Data were classified and analyzed by content analysis approach. RESULTS The data analysis in this study resulted in four main categories and 11 subcategories. The main categories included hope crisis, bad news, cultural diversity, and nurses' limited professional competences. CONCLUSION Results of the present study showed that, white lie told by nurses during patient care may be due to a wide range of patient, nurse and/or organizational related factors. Communication was the main factor that influenced information rendering. Nurses' communication with patients should be based on mutual respect, trust and adequate cultural knowledge, and also nurses should provide precise information to patients, so that they can make accurate decisions regarding their health care.", "gemini": "BỐI CẢNH Giữ cho bệnh nhân khỏe mạnh và được thông tin đầy đủ về chẩn đoán, tiên lượng và phương pháp điều trị là một trong những quyền của bệnh nhân trong bất kỳ hệ thống chăm sóc sức khỏe nào. Mặc dù tất cả các nhà cung cấp dịch vụ chăm sóc sức khỏe đều có cùng quan điểm về việc đưa ra sự thật trong quá trình điều trị, nhưng đôi khi sự thật không được nói với bệnh nhân; đó là lý do tại sao đội ngũ nhân viên y tế lại nói \"dối trắng\". Nghiên cứu này nhằm mục đích khám phá kinh nghiệm của các y tá về việc nói dối trắng trong quá trình chăm sóc bệnh nhân. PHƯƠNG PHÁP Nghiên cứu định tính này được tiến hành từ tháng 6 đến tháng 12 năm 2018. Mười tám y tá bệnh viện đã được tuyển dụng với sự thay đổi tối đa từ mười bệnh viện giáo dục do nhà nước điều hành trực thuộc Đại học Khoa học Y tế Tehran. Lấy mẫu có chủ đích đã được sử dụng và dữ liệu được thu thập bằng các cuộc phỏng vấn bán cấu trúc được tiếp tục cho đến khi dữ liệu bão hòa. Dữ liệu được phân loại và phân tích bằng cách tiếp cận phân tích nội dung. KẾT QUẢ Phân tích dữ liệu trong nghiên cứu này dẫn đến bốn loại chính và 11 loại phụ. Các loại chính bao gồm khủng hoảng hy vọng, tin xấu, sự đa dạng về văn hóa và năng lực chuyên môn hạn chế của y tá. KẾT LUẬN Kết quả của nghiên cứu hiện tại cho thấy rằng, việc y tá nói dối trắng trong quá trình chăm sóc bệnh nhân có thể là do nhiều yếu tố liên quan đến bệnh nhân, y tá và/hoặc tổ chức. Giao tiếp là yếu tố chính ảnh hưởng đến việc truyền tải thông tin. Giao tiếp của y tá với bệnh nhân nên dựa trên sự tôn trọng lẫn nhau, tin tưởng và kiến thức văn hóa đầy đủ, ngoài ra y tá nên cung cấp thông tin chính xác cho bệnh nhân để họ có thể đưa ra quyết định chính xác liên quan đến việc chăm sóc sức khỏe của mình."} +{"idx": 1880139, "en": "BACKGROUND The population of Nigeria is estimated at over 190 million and it is projected to increase by 44% between 2015 and 2030. However, less than one-quarter of women within reproductive age in Nigeria uses modern contraceptive methods despite its importance. Hence, this study aims at examining the influence of individual and community level factors on the use of modern contraceptive method. METHODS The study is a secondary analysis of linked household and Service Delivery Point datasets from a 2018 survey conducted by Performance, Monitoring and Accountability in Nigeria. Data was abstracted for a total of 9126 sexually active women within the ages of 15-49 years across 295 enumeration areas in seven States. A 2-level binary logistic regression was used to examine the association between study variables and the use of modern contraceptives while adjusting for the clustering effect. RESULTS There was significant influence of educational level, marital status, parity, socio-economic status, fertility intention, and awareness of family planning methods on the use of modern contraceptives. Also, women who perceived support from someone in the community on family planning were more likely to use modern contraceptive unlike those without such support. Those who believed that contraceptive methods are used by almost all and some of their friends or relatives were more likely to use modern contraceptive compared to those who think otherwise. CONCLUSIONS The study shows the need to reduce inequalities between FP utilization across women with different socio-economic status as well as increasing the awareness for modern contraceptive methods.", "gemini": "BỐI CẢNH Dân số của Nigeria ước tính hơn 190 triệu người và dự kiến sẽ tăng 44% trong giai đoạn 2015-2030. Tuy nhiên, chưa đến một phần tư phụ nữ trong độ tuổi sinh đẻ ở Nigeria sử dụng các phương pháp tránh thai hiện đại mặc dù nó rất quan trọng. Do đó, nghiên cứu này nhằm mục đích kiểm tra ảnh hưởng của các yếu tố ở cấp độ cá nhân và cộng đồng đối với việc sử dụng phương pháp tránh thai hiện đại. PHƯƠNG PHÁP Nghiên cứu là phân tích thứ cấp các bộ dữ liệu về hộ gia đình và Điểm cung cấp dịch vụ được liên kết từ cuộc khảo sát năm 2018 do Hiệu suất, Giám sát và Trách nhiệm giải trình tại Nigeria thực hiện. Dữ liệu đã được trích xuất cho tổng số 9126 phụ nữ hoạt động tình dục trong độ tuổi từ 15-49 tại 295 khu vực thống kê ở bảy tiểu bang. Hồi quy logistic nhị phân 2 cấp đã được sử dụng để kiểm tra mối liên quan giữa các biến nghiên cứu và việc sử dụng các biện pháp tránh thai hiện đại trong khi điều chỉnh hiệu ứng cụm. KẾT QUẢ Có ảnh hưởng đáng kể của trình độ học vấn, tình trạng hôn nhân, số con, tình trạng kinh tế xã hội, ý định sinh đẻ và nhận thức về các phương pháp kế hoạch hóa gia đình đối với việc sử dụng các biện pháp tránh thai hiện đại. Ngoài ra, những phụ nữ nhận được sự hỗ trợ của ai đó trong cộng đồng về kế hoạch hóa gia đình có nhiều khả năng sử dụng biện pháp tránh thai hiện đại không giống như những người không có sự hỗ trợ như vậy. Những người tin rằng các biện pháp tránh thai được hầu hết mọi người và một số bạn bè hoặc người thân của họ sử dụng có nhiều khả năng sử dụng biện pháp tránh thai hiện đại hơn so với những người nghĩ khác. KẾT LUẬN Nghiên cứu cho thấy cần phải giảm bất bình đẳng giữa việc sử dụng kế hoạch hóa gia đình ở những phụ nữ có tình trạng kinh tế xã hội khác nhau cũng như nâng cao nhận thức về các phương pháp tránh thai hiện đại."} +{"idx": 1880140, "en": "BACKGROUND This study aimed to explore the growth inhibitory effect of myricanol 5-fluorobenzyloxy ether (5FEM) and its underlying mechanisms in human lung adenocarcinoma A549 cells in vitro. METHODS 5FEM was obtained by the chemical modification of myricanol with fluorobenzyloxy ether at the OH(5) position. The cytotoxicity, cell apoptosis, cell cycle, mitochondrial membrane potential (ΔΨm), scratch test, colony formation, and the expression levels of the key survivin pathway-related genes in A549 were evaluated. RESULTS 5FEM could significantly inhibit A549 cell growth; induce cell apoptosis; increase G0/G1 population; reduce ΔΨm; inhibit cell migration and colony formation; upregulate caspase-9, P21, and Bax expression levels; and downregulate PARP, survivin, and Bcl-2 expression level. CONCLUSION These results enhanced our understanding of 5FEM and aid the discovery of novel myricanol derivatives as potential antitumor agents.", "gemini": "BỐI CẢNH Nghiên cứu này nhằm mục đích khám phá tác dụng ức chế tăng trưởng của myricanol 5-fluorobenzyloxy ether (5FEM) và các cơ chế cơ bản của nó trong các tế bào ung thư phổi biểu mô phế quản ở người A549 trong ống nghiệm. PHƯƠNG PHÁP 5FEM thu được bằng cách biến đổi hóa học myricanol với fluorobenzyloxy ether ở vị trí OH(5). Độc tính tế bào, apoptosis tế bào, chu kỳ tế bào, tiềm năng màng ty thể (ΔΨm), xét nghiệm trầy xước, hình thành khuẩn lạc và mức độ biểu hiện của các gen chính liên quan đến con đường survivin trong A549 đã được đánh giá. KẾT QUẢ 5FEM có thể ức chế đáng kể sự phát triển của tế bào A549; gây apoptosis tế bào; tăng quần thể G0/G1; giảm ΔΨm; ức chế di chuyển tế bào và hình thành khuẩn lạc; điều hòa tăng mức biểu hiện caspase-9, P21 và Bax; và điều hòa giảm mức biểu hiện PARP, survivin và Bcl-2. KẾT LUẬN Những kết quả này đã nâng cao sự hiểu biết của chúng tôi về 5FEM và hỗ trợ việc khám phá các dẫn xuất myricanol mới như các tác nhân chống ung thư tiềm năng."} +{"idx": 1880141, "en": "BACKGROUND Fibrosis surrounding cancer cells has been shown to affect cancer cell metastatic behavior. The present study aimed to explore the utility of myxoid stroma as a predictive factor for postoperative relapse in patients with stage II colon cancer. METHODS The present study retrospectively investigated 169 patients who underwent curative surgical resection of stage II colon cancer. The fibrotic stroma was classified according to Ueno's criteria, and the patients were divided into the myxoid (MY) group and the non-MY (NMY) group. We also recorded tumor budding (TB) and investigated the combination of MY and TB for postoperative relapse. Postoperative survival was also explored. RESULTS Thirty-two (18.9%) patients had MY. MY was significantly associated with tumor budding (TB) and postoperative relapse (p <  0.001 and p <  0.001, respectively). The 5-year RFS rates in MY group and NMY group were 52.1 and 94.6% (p < 0.0001), and the 5-year OS rates in MY group and NMY group were 74.6 and 93.3% (p = 0.001). Multivariate analysis showed that both MY and TB were significant risk factors for postoperative relapse (p < 0.001 and p = 0.02, respectively), and that only TB was a significant risk factor for OS (p = 0.043). Furthermore, compared with patients with either one of MY or TB, patients with both MY and TB had postoperative relapse more frequently (11.4% vs. 53.8%). CONCLUSIONS The present study suggests that MY is a predictive marker for postoperative relapse in patients with stage II colon cancer.", "gemini": "BỐI CẢNH Xơ hóa xung quanh tế bào ung thư đã được chứng minh là ảnh hưởng đến hành vi di căn của tế bào ung thư. Nghiên cứu hiện tại nhằm mục đích khám phá tính hữu ích của mô đệm myxoid như một yếu tố dự báo tái phát sau phẫu thuật ở những bệnh nhân ung thư đại tràng giai đoạn II. PHƯƠNG PHÁP Nghiên cứu hiện tại đã nghiên cứu hồi cứu 169 bệnh nhân đã cắt bỏ ung thư đại tràng giai đoạn II bằng phẫu thuật cắt bỏ có mục đích chữa khỏi. Mô đệm xơ hóa được phân loại theo tiêu chuẩn của Ueno và những bệnh nhân được chia thành nhóm myxoid (MY) và nhóm không phải MY (NMY). Chúng tôi cũng ghi lại sự nảy chồi của khối u (TB) và nghiên cứu sự kết hợp của MY và TB đối với tái phát sau phẫu thuật. Sự sống còn sau phẫu thuật cũng được khám phá. KẾT QUẢ Ba mươi hai (18,9%) bệnh nhân có MY. MY có liên quan đáng kể đến sự nảy chồi của khối u (TB) và tái phát sau phẫu thuật (lần lượt là p < 0,001 và p < 0,001). Tỷ lệ RFS 5 năm ở nhóm MY và nhóm NMY lần lượt là 52,1 và 94,6% (p <0,0001) và tỷ lệ OS 5 năm ở nhóm MY và nhóm NMY lần lượt là 74,6 và 93,3% (p = 0,001). Phân tích đa biến cho thấy cả MY và TB đều là những yếu tố nguy cơ đáng kể đối với tái phát sau phẫu thuật (lần lượt là p <0,001 và p = 0,02) và chỉ có TB là yếu tố nguy cơ đáng kể đối với OS (p = 0,043). Hơn nữa, so với những bệnh nhân chỉ có một trong hai yếu tố MY hoặc TB, những bệnh nhân có cả MY và TB có tỷ lệ tái phát sau phẫu thuật cao hơn (11,4% so với 53,8%). KẾT LUẬN Nghiên cứu hiện tại cho thấy MY là một dấu hiệu dự báo tái phát sau phẫu thuật ở những bệnh nhân ung thư đại tràng giai đoạn II."} +{"idx": 1880142, "en": "BACKGROUND Bacterial resistance to antibiotics is a growing health problem that is projected to cause more deaths than cancer by 2050. Consequently, novel antibiotics are urgently needed. Since more than half of the available antibiotics target the structurally conserved bacterial ribosomes, factors involved in protein synthesis are thus prime targets for the development of novel antibiotics. However, experimental identification of these potential antibiotic target proteins can be labor-intensive and challenging, as these proteins are likely to be poorly characterized and specific to few bacteria. Here, we use a bioinformatics approach to identify novel components of protein synthesis. RESULTS In order to identify these novel proteins, we established a Large-Scale Transcriptomic Analysis Pipeline in Crowd (LSTrAP-Crowd), where 285 individuals processed 26 terabytes of RNA-sequencing data of the 17 most notorious bacterial pathogens. In total, the crowd processed 26,269 RNA-seq experiments and used the data to construct gene co-expression networks, which were used to identify more than a hundred uncharacterized genes that were transcriptionally associated with protein synthesis. We provide the identity of these genes together with the processed gene expression data. CONCLUSIONS We identified genes related to protein synthesis in common bacterial pathogens and thus provide a resource of potential antibiotic development targets for experimental validation. The data can be used to explore additional vulnerabilities of bacteria, while our approach demonstrates how the processing of gene expression data can be easily crowd-sourced.", "gemini": "BỐI CẢNH Kháng thuốc của vi khuẩn đối với thuốc kháng sinh là một vấn đề sức khỏe ngày càng gia tăng, dự kiến sẽ gây ra nhiều ca tử vong hơn so với ung thư vào năm 2050. Do đó, rất cần các loại thuốc kháng sinh mới. Vì hơn một nửa số thuốc kháng sinh hiện có nhắm vào ribosome của vi khuẩn được bảo tồn về mặt cấu trúc nên các yếu tố liên quan đến quá trình tổng hợp protein do đó là mục tiêu chính cho việc phát triển các loại thuốc kháng sinh mới. Tuy nhiên, việc xác định bằng thực nghiệm các protein đích kháng sinh tiềm năng này có thể tốn nhiều công sức và đầy thách thức, vì những protein này có khả năng được mô tả kém và chỉ có ở một số ít vi khuẩn. Ở đây, chúng tôi sử dụng phương pháp tin sinh học để xác định các thành phần mới của quá trình tổng hợp protein. KẾT QUẢ Để xác định các protein mới này, chúng tôi đã thiết lập Đường ống phân tích phiên mã quy mô lớn trong đám đông (LSTrAP-Crowd), nơi 285 cá nhân xử lý 26 terabyte dữ liệu giải trình tự RNA của 17 tác nhân gây bệnh ở vi khuẩn khét tiếng nhất. Tổng cộng, đám đông đã xử lý 26.269 thí nghiệm giải trình tự RNA và sử dụng dữ liệu để xây dựng các mạng đồng biểu hiện gen, được sử dụng để xác định hơn một trăm gen chưa được mô tả có liên quan đến phiên mã với quá trình tổng hợp protein. Chúng tôi cung cấp danh tính của các gen này cùng với dữ liệu biểu hiện gen đã xử lý. KẾT LUẬN Chúng tôi đã xác định được các gen liên quan đến quá trình tổng hợp protein ở các tác nhân gây bệnh ở vi khuẩn phổ biến và do đó cung cấp một nguồn các mục tiêu phát triển kháng sinh tiềm năng để xác nhận bằng thực nghiệm. Dữ liệu có thể được sử dụng để khám phá thêm các lỗ hổng của vi khuẩn, trong khi phương pháp tiếp cận của chúng tôi chứng minh cách xử lý dữ liệu biểu hiện gen có thể dễ dàng được thực hiện theo cách cộng đồng."} +{"idx": 1880143, "en": "As medical students, we recognise how the various stages of medical school can influence one's perception of their educational environment, as illustrated by the work of Yoo and Kim. Throughout this article, via the exploration of reviewed literature and personal experience, we provide a critical perspective into the significance of enhancing student happiness within pre-clinical periods of the medical curriculum. Here, we highlight the refinements and safeguards available which we believe should be taken into consideration by educators.", "gemini": "Là sinh viên y khoa, chúng tôi nhận ra cách các giai đoạn khác nhau của trường y có thể ảnh hưởng đến nhận thức của một người về môi trường giáo dục của họ, như được minh họa bởi công trình của Yoo và Kim. Trong suốt bài viết này, thông qua việc khám phá các tài liệu đã được đánh giá và kinh nghiệm cá nhân, chúng tôi cung cấp một góc nhìn phê phán về tầm quan trọng của việc nâng cao hạnh phúc của sinh viên trong các giai đoạn tiền lâm sàng của chương trình giảng dạy y khoa. Tại đây, chúng tôi nêu bật những cải tiến và biện pháp bảo vệ có sẵn mà chúng tôi tin rằng các nhà giáo dục nên cân nhắc."} diff --git a/retranslated_vi_pubmed/chunk_188/error.json b/retranslated_vi_pubmed/chunk_188/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_188/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_189/completed.json b/retranslated_vi_pubmed/chunk_189/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..42b823c88a419e67bcffb89d759752560d3cab05 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_189/completed.json @@ -0,0 +1,146 @@ +[ + 1890000, + 1890001, + 1890002, + 1890003, + 1890004, + 1890005, + 1890006, + 1890007, + 1890008, + 1890009, + 1890010, + 1890011, + 1890012, + 1890013, + 1890014, + 1890015, + 1890016, + 1890017, + 1890018, + 1890019, + 1890020, + 1890021, + 1890022, + 1890023, + 1890024, + 1890025, + 1890026, + 1890027, + 1890028, + 1890029, + 1890030, + 1890031, + 1890032, + 1890033, + 1890034, + 1890035, + 1890036, + 1890037, + 1890038, + 1890039, + 1890040, + 1890041, + 1890042, + 1890043, + 1890044, + 1890045, + 1890046, + 1890047, + 1890048, + 1890049, + 1890050, + 1890051, + 1890052, + 1890053, + 1890054, + 1890055, + 1890056, + 1890057, + 1890058, + 1890059, + 1890060, + 1890061, + 1890062, + 1890063, + 1890064, + 1890065, + 1890066, + 1890067, + 1890068, + 1890069, + 1890070, + 1890071, + 1890072, + 1890073, + 1890074, + 1890075, + 1890076, + 1890077, + 1890078, + 1890079, + 1890080, + 1890081, + 1890082, + 1890083, + 1890084, + 1890085, + 1890086, + 1890087, + 1890088, + 1890089, + 1890090, + 1890091, + 1890092, + 1890093, + 1890094, + 1890095, + 1890096, + 1890097, + 1890098, + 1890099, + 1890100, + 1890101, + 1890102, + 1890103, + 1890104, + 1890105, + 1890106, + 1890107, + 1890108, + 1890109, + 1890110, + 1890111, + 1890112, + 1890113, + 1890114, + 1890115, + 1890116, + 1890117, + 1890118, + 1890119, + 1890120, + 1890121, + 1890122, + 1890123, + 1890124, + 1890125, + 1890126, + 1890127, + 1890128, + 1890129, + 1890130, + 1890131, + 1890132, + 1890133, + 1890134, + 1890135, + 1890136, + 1890137, + 1890138, + 1890139, + 1890140, + 1890141, + 1890142, + 1890143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_189/data.jsonl b/retranslated_vi_pubmed/chunk_189/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..22e2cc4a950aaf5077a409925f5670d3ac819758 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_189/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1890000, "en": "Stem cells are essential for tissue homeostasis and regeneration throughout the lifespan of multicellular organisms. The decline in stem cell function during advanced age is associated with a reduced regenerative potential of tissues that leads to an increased frequency of diseases. Age-related changes also occur in the dental pulp that represents a reliable model tissue, with high regenerative capability, for studying senescence mechanisms. However, little information is available concerning the effects of ageing on dental stem-cell function. In this mini-review, recent data on how the molecular and functional alterations that accumulate in stem cell populations during ageing result in modifications of dental pulp physiology are discussed. Changes that accumulate during ageing such as how reduction of pulp chamber volume, decreased vascular supply and modifications to the stem cell niches affect stem cell functions and, therefore, dental pulp regenerative potential in response to various stressful agents. Dental pulp cells from aged individuals are still metabolically active and secrete pro-inflammatory and matrix-degrading molecules. Furthermore, miRNAs and exosomes derived from dental pulp stem cells constitute an attractive source of nanovesicles for the treatment of age-related dental pathologies. Further investigation of the epigenetic alterations in dental pulp stem cells, accumulating during ageing, might reveal crucial information for potential stem cell-based therapeutic approaches in the elderly.", "gemini": "Tế bào gốc rất cần thiết cho sự cân bằng nội môi và tái tạo mô trong suốt cuộc đời của các sinh vật đa bào. Sự suy giảm chức năng của tế bào gốc trong thời kỳ tuổi già có liên quan đến tiềm năng tái tạo giảm của các mô dẫn đến tần suất mắc bệnh tăng lên. Những thay đổi liên quan đến tuổi tác cũng xảy ra ở tủy răng, đây là mô mô hình đáng tin cậy, có khả năng tái tạo cao, để nghiên cứu cơ chế lão hóa. Tuy nhiên, có ít thông tin về tác động của lão hóa đối với chức năng tế bào gốc răng. Trong bài tổng quan nhỏ này, các dữ liệu gần đây về cách các biến đổi về mặt phân tử và chức năng tích tụ trong quần thể tế bào gốc trong quá trình lão hóa dẫn đến những thay đổi về sinh lý tủy răng được thảo luận. Những thay đổi tích tụ trong quá trình lão hóa như giảm thể tích buồng tủy, giảm cung cấp mạch máu và những thay đổi ở các hốc tế bào gốc ảnh hưởng đến chức năng tế bào gốc như thế nào và do đó, tiềm năng tái tạo tủy răng để đáp ứng với các tác nhân gây căng thẳng khác nhau. Các tế bào tủy răng từ những người cao tuổi vẫn hoạt động về mặt trao đổi chất và tiết ra các phân tử tiền viêm và phân hủy ma trận. Hơn nữa, các miRNA và exosome có nguồn gốc từ tế bào gốc tủy răng tạo thành một nguồn nanovesicle hấp dẫn để điều trị các bệnh lý răng miệng liên quan đến tuổi tác. Việc tiếp tục nghiên cứu các biến đổi biểu sinh ở tế bào gốc tủy răng, tích tụ trong quá trình lão hóa, có thể tiết lộ thông tin quan trọng cho các phương pháp điều trị tiềm năng dựa trên tế bào gốc ở người cao tuổi."} +{"idx": 1890001, "en": "PURPOSE Vesicourethral anastomosis (VUA) represents a challenging step of open radical prostatectomy (ORP) because of limitation of space in the depth of male pelvis, lack of control on knots during tightening which subse-quently causes inadequate coupling of VUA or breakdown of knots, and also extremely difficult reapplication of sutures. To facilitate this step of ORP, we have developed a simple and reproducible technique and reported our 8-year experience. MATERIALS AND METHODS We used two extra-long DeBakey tissue forceps to approximate the bladder neck to the urethral stump. We found it more beneficial than Babcock clamp especially in obese patients with excess fatty tissue in the pelvic area. In this technique, the surgeon's assistant creates more space for the surgeon's hand by sweeping the fatty tissue away from the anastomotic area and then pushes the reconstructed bladder neck down while the sutures are being tied. RESULTS We analyzed data from 200 patients with prostatic cancer who underwent open radical prostatectomy performed from 2009 to 2017. There were only 2 sutures disrupted during knot tying. In two cases (1%), drain output was more than 30 mL/day on postoperative day 2 and drainage was left in place for a longer duration. With the help of medications, time voiding and dedicated pelvic floor exercise whenever needed,.the goal of full urinary continence (0- 1 pad/day) was achieved in 85%, 94% and 98% of patients immediately after catheter removal, 3 months and 6 months after surgery, respectively. Eight patients (4%) developed urethral stricture. CONCLUSION The surgical technique has been shown to be an independent predictor of urinary continence. We introduce a new simple modification of vesicourethral anastomosis during RP. Using this technique; in addition to reducing anastomotic disruption rate and increasing knot tying control, postoperative urinary continence after ORP may also be improved.", "gemini": "MỤC ĐÍCH Phẫu thuật nối niệu đạo bàng quang (VUA) là một bước đầy thách thức của phẫu thuật cắt bỏ tuyến tiền liệt mở rộng (ORP) do hạn chế về không gian ở độ sâu của xương chậu nam, không kiểm soát được các nút thắt trong quá trình thắt chặt, gây ra tình trạng nối VUA không đủ hoặc các nút thắt bị đứt, và cũng vô cùng khó khăn khi khâu lại. Để tạo thuận lợi cho bước ORP này, chúng tôi đã phát triển một kỹ thuật đơn giản và dễ tái tạo và báo cáo kinh nghiệm 8 năm của mình. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi sử dụng hai kẹp mô DeBakey cực dài để xấp xỉ cổ bàng quang với gốc niệu đạo. Chúng tôi thấy nó có lợi hơn so với kẹp Babcock, đặc biệt là ở những bệnh nhân béo phì có nhiều mô mỡ thừa ở vùng xương chậu. Trong kỹ thuật này, trợ lý phẫu thuật tạo nhiều không gian hơn cho bàn tay của bác sĩ phẫu thuật bằng cách quét mô mỡ ra khỏi vùng nối và sau đó đẩy cổ bàng quang đã tái tạo xuống trong khi thắt các mũi khâu. KẾT QUẢ Chúng tôi đã phân tích dữ liệu từ 200 bệnh nhân ung thư tuyến tiền liệt đã phẫu thuật cắt bỏ tuyến tiền liệt mở rộng từ năm 2009 đến năm 2017. Chỉ có 2 mũi khâu bị đứt trong quá trình thắt nút. Trong hai trường hợp (1%), lượng dịch dẫn lưu nhiều hơn 30 mL/ngày vào ngày thứ 2 sau phẫu thuật và dẫn lưu được giữ nguyên trong thời gian dài hơn. Với sự trợ giúp của thuốc, thời gian đi tiểu và các bài tập sàn chậu chuyên dụng khi cần thiết, mục tiêu kiểm soát tiểu tiện hoàn toàn (0-1 miếng lót/ngày) đạt được ở 85%, 94% và 98% bệnh nhân ngay sau khi tháo thông tiểu, 3 tháng và 6 tháng sau phẫu thuật. Tám bệnh nhân (4%) bị hẹp niệu đạo. KẾT LUẬN Kỹ thuật phẫu thuật đã được chứng minh là một yếu tố dự đoán độc lập về khả năng kiểm soát tiểu tiện. Chúng tôi giới thiệu một sửa đổi đơn giản mới về phẫu thuật nối niệu đạo bàng quang trong RP. Sử dụng kỹ thuật này; ngoài việc giảm tỷ lệ đứt nối và tăng khả năng kiểm soát thắt nút, khả năng kiểm soát tiểu tiện sau phẫu thuật sau ORP cũng có thể được cải thiện."} +{"idx": 1890002, "en": "Lower limb external maximal power output capacity is a key physical component of performance in many sports. During squat jump and countermovement jump tests, athletes produce high amounts of mechanical work over a short duration to displace their body mass (i.e. the dimension of mechanical power). Thus, jump height has been frequently used by the sports science and medicine communities as an indicator of the power output produced during the jump and by extension, of maximal power output capacity. However, in this article, we contend that squat jump and countermovement jump height are not systematically good indicators of power output produced during the jump and maximal power output capacity. To support our opinion, we first detail why, theoretically, jump height and maximal power output capacity are not fully related. Specifically, we demonstrate that individual body mass, push-off distance, optimal loading and the force-velocity profile confound the jump height-power relationship. We also discuss the relationship between squat jump or countermovement jump height and maximal power output capacity measured with a force plate based on data reported in the literature, which added to our own experimental evidence. Finally, we discuss the limitations of existing practical solutions (regression-based estimation equations and allometric scaling), and advocate using a valid, reliable and simple field-based procedure to compute individual power output produced during the jump and maximal power output capacity directly from jump height, body mass and push-off distance. The latter may allow researchers and practitioners to reduce bias in their assessment of lower limb mechanical power output by using jump height as an input with a simple yet accurate computation method, and not as the first/only variable of interest.", "gemini": "Khả năng sản xuất công suất tối đa bên ngoài chi dưới là một thành phần vật lý quan trọng của hiệu suất trong nhiều môn thể thao. Trong các bài kiểm tra nhảy ngồi và nhảy đối kháng, các vận động viên tạo ra một lượng lớn công cơ học trong thời gian ngắn để di chuyển khối lượng cơ thể của họ (tức là kích thước của công cơ học). Do đó, chiều cao nhảy thường được các cộng đồng khoa học thể thao và y học sử dụng như một chỉ báo về công suất đầu ra được tạo ra trong khi nhảy và mở rộng, về khả năng sản xuất công suất tối đa. Tuy nhiên, trong bài viết này, chúng tôi cho rằng chiều cao nhảy ngồi và nhảy đối kháng không phải là chỉ số tốt một cách có hệ thống về công suất đầu ra được tạo ra trong khi nhảy và khả năng sản xuất công suất tối đa. Để hỗ trợ quan điểm của mình, trước tiên chúng tôi trình bày chi tiết lý do tại sao, về mặt lý thuyết, chiều cao nhảy và khả năng sản xuất công suất tối đa không liên quan đầy đủ. Cụ thể, chúng tôi chứng minh rằng khối lượng cơ thể riêng lẻ, khoảng cách đẩy, tải tối ưu và cấu hình lực-vận tốc làm phức tạp mối quan hệ chiều cao nhảy-công suất. Chúng tôi cũng thảo luận về mối quan hệ giữa chiều cao nhảy ngồi hoặc nhảy đối kháng và khả năng sản xuất công suất tối đa được đo bằng lực kế dựa trên dữ liệu được báo cáo trong tài liệu, được thêm vào bằng chứng thực nghiệm của riêng chúng tôi. Cuối cùng, chúng tôi thảo luận về những hạn chế của các giải pháp thực tế hiện có (phương trình ước tính dựa trên hồi quy và tỷ lệ dị hình) và ủng hộ việc sử dụng một quy trình dựa trên thực địa hợp lệ, đáng tin cậy và đơn giản để tính toán công suất đầu ra riêng lẻ được tạo ra trong khi nhảy và khả năng sản xuất công suất tối đa trực tiếp từ chiều cao nhảy, khối lượng cơ thể và khoảng cách đẩy. Phương pháp sau có thể cho phép các nhà nghiên cứu và bác sĩ lâm sàng giảm sai lệch trong đánh giá công suất đầu ra cơ học của chi dưới bằng cách sử dụng chiều cao nhảy làm đầu vào với phương pháp tính toán đơn giản nhưng chính xác, chứ không phải là biến đầu tiên/duy nhất được quan tâm."} +{"idx": 1890003, "en": "INTRODUCTION Vertical immersion induces a variety of physiological responses in different body systems, depending on the properties of fluid mechanics, which are the basis that underpins aquatic therapy programs in different pathologies. AIM To perform a systematic review to analyze and describe the effects that vertical immersion produces on the nervous system in healthy subjects. SUBJECTS AND METHODS A systematic search of the existing literature was conducted in the databases BRAIN, PubMed, PEDro and Web of Science. Quality was methodologically assessed using the CASPe guideline and the level of evidence was categorized using the Oxford scale. A total of 12 articles were included, with a score range of 7-10 according to CASPe, levels of evidence 1b-2b and grade of recommendation B. RESULTS All studies showed positive results to the different forms of exposure of vertical immersion in water and the summation of the stimuli used; no adverse effects were reported in any case. CONCLUSIONS The vertical immersion in the water generates positive effects on cerebral blood flows, cortical activation, executive functions and the production of neurotrophins in healthy subjects.", "gemini": "GIỚI THIỆU Ngâm mình theo chiều dọc gây ra nhiều phản ứng sinh lý khác nhau ở các hệ thống cơ thể khác nhau, tùy thuộc vào các đặc tính của cơ học chất lỏng, là cơ sở làm nền tảng cho các chương trình trị liệu dưới nước trong các bệnh lý khác nhau. MỤC ĐÍCH Thực hiện đánh giá có hệ thống để phân tích và mô tả các tác động mà ngâm mình theo chiều dọc tạo ra trên hệ thần kinh ở những đối tượng khỏe mạnh. ĐỐI TƯỢNG VÀ PHƯƠNG PHÁP Một tìm kiếm có hệ thống các tài liệu hiện có đã được thực hiện trong các cơ sở dữ liệu BRAIN, PubMed, PEDro và Web of Science. Chất lượng được đánh giá theo phương pháp luận sử dụng hướng dẫn CASPe và mức độ bằng chứng được phân loại theo thang điểm Oxford. Tổng cộng có 12 bài báo được đưa vào, với phạm vi điểm từ 7-10 theo CASPe, mức độ bằng chứng 1b-2b và mức độ khuyến nghị B. KẾT QUẢ Tất cả các nghiên cứu đều cho thấy kết quả tích cực đối với các hình thức tiếp xúc khác nhau của việc ngâm mình theo chiều dọc trong nước và tổng hợp các kích thích được sử dụng; không có tác dụng phụ nào được báo cáo trong bất kỳ trường hợp nào. KẾT LUẬN Việc ngâm mình theo chiều dọc trong nước tạo ra những tác động tích cực đến lưu lượng máu não, hoạt động vỏ não, chức năng điều hành và sản xuất các chất dinh dưỡng thần kinh ở những đối tượng khỏe mạnh."} +{"idx": 1890004, "en": "INTRODUCTION There is a growing need for scientific studies and tools that allow the evaluation of the effects at an affective level of some non-pharmacological therapies for people with dementia such as the creative therapeutic dance. AIMS To explore the validity and reliability of the Profile of Mood States (POMS) in people with dementia and to analyse the possible differences in the scores of the participants before and after sessions of creative therapeutic dance. PATIENTS AND METHODS POMS was administered to 36 people with dementia before and after four group sessions of creative therapeutic dance. To verify the construct validity, PANAS and STAXI-2 were also administered. RESULTS The POMS presented a good internal consistency for each factor and an excellent one for the total scale. The temporal stability was medium-high. Convergent validity results support construct validity. After the creative therapeutic dance sessions, POMS factors tension, depression and confusion were reduced, while vigour increased significantly. There was no effect on fatigue and anger. CONCLUSIONS The validity and reliability of POMS have been demonstrated in a population with dementia. Creative therapeutic dance can offer emotional benefits for people with dementia.", "gemini": "GIỚI THIỆU Có nhu cầu ngày càng tăng đối với các nghiên cứu khoa học và các công cụ cho phép đánh giá các tác động ở cấp độ tình cảm của một số liệu pháp không dùng thuốc đối với những người mắc chứng mất trí như khiêu vũ trị liệu sáng tạo. MỤC ĐÍCH Khám phá tính hợp lệ và độ tin cậy của Hồ sơ trạng thái tâm trạng (POMS) ở những người mắc chứng mất trí và phân tích sự khác biệt có thể có về điểm số của những người tham gia trước và sau các buổi khiêu vũ trị liệu sáng tạo. BỆNH NHÂN VÀ PHƯƠNG PHÁP POMS đã được thực hiện đối với 36 người mắc chứng mất trí trước và sau bốn buổi khiêu vũ trị liệu sáng tạo theo nhóm. Để xác minh tính hợp lệ của cấu trúc, PANAS và STAXI-2 cũng được thực hiện. KẾT QUẢ POMS thể hiện tính nhất quán nội tại tốt cho từng yếu tố và tính nhất quán tuyệt vời cho toàn bộ thang điểm. Tính ổn định theo thời gian ở mức trung bình đến cao. Kết quả hợp lệ hội tụ hỗ trợ tính hợp lệ của cấu trúc. Sau các buổi khiêu vũ trị liệu sáng tạo, các yếu tố căng thẳng, trầm cảm và lú lẫn của POMS đã giảm, trong khi sức mạnh tăng lên đáng kể. Không có ảnh hưởng đến sự mệt mỏi và tức giận. KẾT LUẬN Tính hợp lệ và độ tin cậy của POMS đã được chứng minh trong quần thể mắc chứng mất trí. Khiêu vũ trị liệu sáng tạo có thể mang lại lợi ích về mặt cảm xúc cho những người mắc chứng mất trí."} +{"idx": 1890005, "en": "There was a time when clinicians looked down on any decision-making that did not depend on their own judgement. Statisticians laughed at such naivety, as they were fully aware of the extent to which the heuristics that guide human decisions are capable of error, even in the most highly trained individuals. More than 60 years after the beginning of this conflict, the two standpoints have still not been adopted in clinical psychology or in neuropsychology. This work defends the practical application of statistics in clinical decision-making and shows that even intuitive-type judgement is somehow based on applying the basic use of statistical concepts, without neglecting the fact that statistical instruments have sometimes been misused. To this end, this study compares the opinions offered by some clinicians in sentence form with the bibliography consulted, which spans the period from the earliest works by the clinical psychologist Paul Meehl to the present day. We have no intention whatsoever of imposing an opinion, but instead of discussing the advantage of adopting an approach that uses mathematical knowledge to support clinical decision-making. Attention is also drawn to the need for multidisciplinary teams in the special units of our hospitals.", "gemini": "Đã có thời các bác sĩ lâm sàng coi thường bất kỳ quyết định nào không phụ thuộc vào phán đoán của riêng họ. Các nhà thống kê cười nhạo sự ngây thơ như vậy, vì họ hoàn toàn nhận thức được phạm vi mà các phương pháp tiếp cận hướng dẫn các quyết định của con người có khả năng gây ra lỗi, ngay cả ở những cá nhân được đào tạo bài bản nhất. Hơn 60 năm sau khi bắt đầu cuộc xung đột này, hai quan điểm vẫn chưa được áp dụng trong tâm lý học lâm sàng hoặc trong tâm lý học thần kinh. Công trình này bảo vệ ứng dụng thực tế của thống kê trong việc ra quyết định lâm sàng và cho thấy rằng ngay cả phán đoán theo kiểu trực quan cũng phần nào dựa trên việc áp dụng các khái niệm thống kê cơ bản, mà không bỏ qua thực tế là các công cụ thống kê đôi khi đã bị sử dụng sai. Để đạt được mục đích này, nghiên cứu này so sánh các ý kiến do một số bác sĩ lâm sàng đưa ra dưới dạng câu với các tài liệu tham khảo được tham khảo, bao gồm giai đoạn từ những tác phẩm đầu tiên của nhà tâm lý học lâm sàng Paul Meehl cho đến ngày nay. Chúng tôi không có ý định áp đặt bất kỳ ý kiến nào, mà thay vào đó là thảo luận về lợi thế của việc áp dụng một phương pháp tiếp cận sử dụng kiến thức toán học để hỗ trợ việc ra quyết định lâm sàng. Cũng cần lưu ý đến nhu cầu về các nhóm đa ngành trong các đơn vị đặc biệt của bệnh viện chúng ta."} +{"idx": 1890006, "en": "The fragile X associated tremor/ataxia syndrome (FXTAS) is a neurodegenerative disease associated with the repetition of CGG triplets (55-200 CGG repetitions) in the FMR1 gene. The premutation of the FMR1 gene, contrasting with the full mutation (more than 200 CGG repetitions), presents an increased production of messenger and a similar or slightly decreased production of FMRP protein. FXTAS affects 40% of men and 16% of women carriers of the premutation. It presents with a wide constellation of neurological signs such as intention tremor, cerebellar ataxia, parkinsonism, executive function deficits, peripheral neuropathy and cognitive decline leading to dementia among others. In this review, we present what is currently known about the molecular mechanism, the radiological findings and the pathology, as well as the complexity of the diagnosis and management of FXTAS.", "gemini": "Hội chứng run/rối vận động liên quan đến X dễ vỡ (FXTAS) là một bệnh thoái hóa thần kinh liên quan đến sự lặp lại của bộ ba CGG (55-200 lần lặp lại CGG) trong gen FMR1. Sự đột biến trước của gen FMR1, trái ngược với đột biến hoàn toàn (hơn 200 lần lặp lại CGG), biểu hiện sự gia tăng sản xuất chất truyền tin và sự sản xuất protein FMRP tương tự hoặc giảm nhẹ. FXTAS ảnh hưởng đến 40% nam giới và 16% nữ giới mang đột biến trước. Nó biểu hiện với một loạt các dấu hiệu thần kinh như run cố ý, mất điều hòa tiểu não, bệnh Parkinson, thiếu hụt chức năng điều hành, bệnh thần kinh ngoại biên và suy giảm nhận thức dẫn đến mất trí nhớ. Trong bài đánh giá này, chúng tôi trình bày những hiểu biết hiện tại về cơ chế phân tử, các phát hiện về hình ảnh học và bệnh lý, cũng như sự phức tạp của chẩn đoán và quản lý FXTAS."} +{"idx": 1890007, "en": "Lobophora is a common tropical to temperate genus of brown algae found in a plethora of habitats including shallow and deep-water coral reefs, rocky shores, mangroves, seagrass beds, and rhodoliths beds. Recent molecular studies have revealed that Lobophora species diversity has been severely underestimated. Current estimates of the species numbers range from 100 to 140 species with a suggested center of diversity in the Central Indo-Pacific. This study used three molecular markers (cox3, rbcL, psbA), different single-marker species delimitation methods (GMYC, ABGD, PTP), and morphological evidence to evaluate Lobophora species diversity in the Western Atlantic and the Eastern Pacific oceans. Cox3 provided the greatest number of primary species hypotheses(PSH), followed by rbcL and then psbA. GMYC species delimitation analysis was the most conservative across all three markers, followed by PTP, and then ABGD. The most informative diagnostic morphological characters were thallus thickness and number of cell layers in both the medulla and the dorsal/ventral cortices. Following a consensus approach, 14 distinct Lobophora species were identified in the Western Atlantic and five in the Eastern Pacific. Eight new species from these two oceans were herein described: L. adpressa sp. nov., L. cocoensis sp. nov., L. colombiana sp. nov., L. crispata sp. nov., L. delicata sp. nov., L. dispersa sp. nov., L. panamensis sp. nov., and L. tortugensis sp. nov. This study showed that the best approach to confidently identify Lobophora species is to analyze DNA sequences (preferably cox3 and rbcL) followed by comparative morphological and geographical assessment.", "gemini": "Lobophora là một chi tảo nâu nhiệt đới đến ôn đới phổ biến được tìm thấy ở vô số môi trường sống bao gồm các rạn san hô nước nông và nước sâu, bờ đá, rừng ngập mặn, thảm cỏ biển và thảm rhodolith. Các nghiên cứu phân tử gần đây đã tiết lộ rằng sự đa dạng loài của Lobophora đã bị đánh giá thấp nghiêm trọng. Ước tính hiện tại về số lượng loài dao động từ 100 đến 140 loài với một trung tâm đa dạng được đề xuất ở Trung Ấn Độ - Thái Bình Dương. Nghiên cứu này đã sử dụng ba dấu hiệu phân tử (cox3, rbcL, psbA), các phương pháp phân định loài dấu hiệu đơn lẻ khác nhau (GMYC, ABGD, PTP) và bằng chứng hình thái để đánh giá sự đa dạng loài Lobophora ở Tây Đại Tây Dương và Đông Thái Bình Dương. Cox3 cung cấp số lượng giả thuyết loài chính (PSH) lớn nhất, tiếp theo là rbcL và sau đó là psbA. Phân tích phân định loài GMYC là bảo thủ nhất trong cả ba dấu hiệu, tiếp theo là PTP, sau đó là ABGD. Các đặc điểm hình thái chẩn đoán nhiều thông tin nhất là độ dày của thallus và số lớp tế bào ở cả tủy và vỏ não lưng/bụng. Theo một cách tiếp cận thống nhất, 14 loài Lobophora riêng biệt đã được xác định ở Tây Đại Tây Dương và năm loài ở Đông Thái Bình Dương. Tám loài mới từ hai đại dương này được mô tả ở đây: L. adpressa sp. nov., L. cocoensis sp. nov., L. colombiana sp. nov., L. crispata sp. nov., L. delicata sp. nov., L. dispersa sp. nov., L. panamensis sp. nov. và L. tortugensis sp. nov. Nghiên cứu này cho thấy cách tiếp cận tốt nhất để xác định chắc chắn các loài Lobophora là phân tích trình tự DNA (tốt nhất là cox3 và rbcL) tiếp theo là đánh giá hình thái và địa lý so sánh."} +{"idx": 1890008, "en": "Spirogyra filaments show unique photomovement that differs in response to blue, red, and far-red light. Phototropins involved in the blue-light movement have been characterized together with downstream signaling components, but the photoreceptors and mechanical effectors of red- and far-red light movement are not yet characterized. The filaments of Spirogyra varians slowly bent and aggregated to form a tangled mass in red light. In far-red light, the filaments unbent, stretched rapidly, and separated from each other. Mannitol and/or sorbitol treatment significantly inhibited this far-red light movement suggesting that turgor pressure is the driving force of this movement. The bending and aggregating movements of filaments in red light were not affected by osmotic change. Three phytochrome homologues isolated from S. varians showed unique phylogenetic characteristics. Two canonical phytochromes, named SvPHY1 and SvPHY2, and a noncanonical phytochrome named SvPHYX2. SvPHY1 is the first PHY1 family phytochrome reported in zygnematalean algae. The gene involved in the transport of phytochromes into the nucleus was characterized, and its expression in response to red and far-red light was measured using quantitative PCR. Our results suggest that the phytochromes and the genes involved in the transport system into the nucleus are well conserved in S. varians.", "gemini": "Các sợi Spirogyra biểu hiện chuyển động quang độc đáo khác nhau khi phản ứng với ánh sáng xanh, đỏ và xa đỏ. Các quang sắc tố liên quan đến chuyển động ánh sáng xanh đã được đặc trưng cùng với các thành phần truyền tín hiệu hạ nguồn, nhưng các thụ thể quang và các tác nhân cơ học của chuyển động ánh sáng đỏ và xa đỏ vẫn chưa được đặc trưng. Các sợi của Spirogyra varians từ từ uốn cong và tập hợp lại thành một khối rối trong ánh sáng đỏ. Trong ánh sáng xa đỏ, các sợi duỗi thẳng, kéo dài nhanh chóng và tách rời nhau. Xử lý bằng mannitol và/hoặc sorbitol ức chế đáng kể chuyển động ánh sáng xa đỏ này, cho thấy áp suất trương nước là động lực của chuyển động này. Các chuyển động uốn cong và tập hợp của các sợi trong ánh sáng đỏ không bị ảnh hưởng bởi sự thay đổi thẩm thấu. Ba loại tương đồng phytochrome được phân lập từ S. varians cho thấy các đặc điểm phát sinh loài độc đáo. Hai phytochrome điển hình, có tên là SvPHY1 và SvPHY2, và một phytochrome không điển hình có tên là SvPHYX2. SvPHY1 là phytochrome đầu tiên của họ PHY1 được báo cáo trong tảo zygnematalean. Gen liên quan đến quá trình vận chuyển phytochrome vào nhân đã được đặc trưng và biểu hiện của nó khi phản ứng với ánh sáng đỏ và xa đỏ đã được đo bằng PCR định lượng. Kết quả của chúng tôi cho thấy rằng các phytochrome và các gen liên quan đến hệ thống vận chuyển vào nhân được bảo tồn tốt trong S. varians."} +{"idx": 1890009, "en": "Carbohydrates are dietary nutrients that have an influence on cells physiology, cell reproductive capacity and, consequently, the lifespan of organisms. They are used in cellular processes after conversion to glucose, which is the primary source of energy and carbon skeleton for biosynthetic processes. Studies of the influence of glucose on cellular parameters and lifespan of organisms are primarily concerned with the effect of low glucose concentration defined as calorie restriction conditions. However, the effect of high glucose concentration on cell physiology is also very important. Thus, a comparative analysis of the effects of low and high glucose concentration conditions on cell efficiency was proposed with regard to reproductive capacity and total lifespan of the cell. Glucose concentration determines the type of metabolism and biosynthetic capabilities, which in turn, through the regulation on the cell size, may affect the reproductive capacity of cells. This study was conducted on yeast cells of wild-type and mutant strains Δgpa2 and Δgpr1 with glucose signalling pathway impairment. Such an experimental model enabled testing both the role of glucose concentration in the regulation of metabolic changes and the extent to which these changes depend on the extracellular or intracellular glucose concentrations. It has been shown here that calorie/glucose excess connected with changes in cell metabolic fluxes increases biosynthetic capabilities of yeast cells. This leads to an increase in cell dry weight accompanied by the increase in cell size and a simultaneous decrease in the reproductive potential and the overall length of cell life.", "gemini": "Carbohydrate là chất dinh dưỡng trong chế độ ăn uống có ảnh hưởng đến sinh lý tế bào, khả năng sinh sản của tế bào và do đó, tuổi thọ của các sinh vật. Chúng được sử dụng trong các quá trình tế bào sau khi chuyển đổi thành glucose, đây là nguồn năng lượng chính và bộ khung cacbon cho các quá trình sinh tổng hợp. Các nghiên cứu về ảnh hưởng của glucose đối với các thông số tế bào và tuổi thọ của các sinh vật chủ yếu liên quan đến tác động của nồng độ glucose thấp được định nghĩa là điều kiện hạn chế calo. Tuy nhiên, tác động của nồng độ glucose cao đối với sinh lý tế bào cũng rất quan trọng. Do đó, một phân tích so sánh về tác động của các điều kiện nồng độ glucose thấp và cao đối với hiệu quả của tế bào đã được đề xuất liên quan đến khả năng sinh sản và tuổi thọ của tế bào. Nồng độ glucose xác định loại chuyển hóa và khả năng sinh tổng hợp, từ đó, thông qua quá trình điều chỉnh kích thước tế bào, có thể ảnh hưởng đến khả năng sinh sản của tế bào. Nghiên cứu này được tiến hành trên các tế bào nấm men của các chủng đột biến kiểu hoang dã và Δgpa2 và Δgpr1 với sự suy giảm đường truyền tín hiệu glucose. Một mô hình thực nghiệm như vậy cho phép kiểm tra cả vai trò của nồng độ glucose trong việc điều chỉnh các thay đổi chuyển hóa và mức độ mà những thay đổi này phụ thuộc vào nồng độ glucose ngoại bào hoặc nội bào. Người ta đã chứng minh ở đây rằng lượng calo / glucose dư thừa kết nối với những thay đổi trong dòng chuyển hóa tế bào làm tăng khả năng sinh tổng hợp của các tế bào nấm men. Điều này dẫn đến sự gia tăng trọng lượng khô của tế bào đi kèm với sự gia tăng kích thước tế bào và đồng thời giảm khả năng sinh sản và tuổi thọ của tế bào."} +{"idx": 1890010, "en": "OBJECTIVE To determine whether ovarian reserve is compromised after hysterectomy with bilateral salpingectomy. METHODS A prospective longitudinal study was conducted among 84 women who underwent hysterectomy with bilateral salpingectomy at a tertiary medical center in Beijing, China, between August 2, 2015, and January 15, 2017. Serum levels of anti-Müllerian hormone (AMH) and follicle-stimulating hormone (FSH) were measured to assess ovarian function before undergoing the procedure (baseline) and at weeks 1 and 6 after surgery (postoperative period). RESULTS The median age was 41.61 ± 0.62 years. Age negatively correlated with the serum AMH level at baseline (P<0.001), as well as with preoperative-to-postoperative changes in the concentration of this hormone (P<0.001). Serum AMH levels were lower in the postoperative period versus the preoperative period (P<0.001). By contrast, serum FSH levels were higher in the postoperative period than in the preoperative period (P<0.001). Moreover, no correlation was found with body mass index. CONCLUSIONS Hysterectomy with bilateral salpingectomy compromised ovarian reserve, with the damage being most severe among younger patients.", "gemini": "MỤC TIÊU Xác định xem buồng trứng có bị tổn thương sau khi cắt tử cung với cắt vòi trứng hai bên không. PHƯƠNG PHÁP Một nghiên cứu theo chiều dọc có triển vọng đã được tiến hành trên 84 phụ nữ đã cắt tử cung với cắt vòi trứng hai bên tại một trung tâm y tế bậc ba ở Bắc Kinh, Trung Quốc, từ ngày 2 tháng 8 năm 2015 đến ngày 15 tháng 1 năm 2017. Nồng độ hormone chống Müller (AMH) và hormone kích thích nang trứng (FSH) trong huyết thanh được đo để đánh giá chức năng buồng trứng trước khi tiến hành thủ thuật (giá trị cơ bản) và vào tuần thứ 1 và tuần thứ 6 sau phẫu thuật (giai đoạn hậu phẫu). KẾT QUẢ Độ tuổi trung bình là 41,61 ± 0,62 tuổi. Tuổi có mối tương quan nghịch với nồng độ AMH trong huyết thanh lúc ban đầu (P <0,001), cũng như với những thay đổi trước và sau phẫu thuật về nồng độ của loại hormone này (P <0,001). Nồng độ AMH trong huyết thanh thấp hơn trong giai đoạn hậu phẫu so với giai đoạn trước phẫu thuật (P <0,001). Ngược lại, nồng độ FSH trong huyết thanh cao hơn trong giai đoạn hậu phẫu so với giai đoạn trước phẫu thuật (P <0,001). Hơn nữa, không tìm thấy mối tương quan với chỉ số khối cơ thể. KẾT LUẬN Cắt tử cung với cắt vòi trứng hai bên làm tổn thương buồng trứng, với mức độ tổn thương nghiêm trọng nhất ở những bệnh nhân trẻ tuổi."} +{"idx": 1890011, "en": "Infectious haematopoietic necrosis virus (IHNV) is the causative agent of infectious haematopoietic necrosis, a disease of salmonid responsible for great economic losses. The disease occurs in most parts of the world where rainbow trout is reared but has not been previously reported in Kenya. In this study, rainbow trout fry and growers from two farms in Nyeri County were screened for IHNV. Whole fry (n = 4 from each farm) and kidney samples from growers (n = 15 and n = 6 from the two farms, respectively) were collected and preserved for cell culture examination or PCR analysis. Screening of samples was done by PCR followed by sequencing of the glycoprotein gene of the virus. Demonstration of the virus was done by propagation in EPC cells followed by the indirect fluorescence antibody test (IFAT). The results revealed the presence of IHNV at low prevalence of 0.1 and 0.4 for the two farms. The virus was confirmed both by IFAT and by partial sequencing of the G gene. Phylogenetic analysis revealed that the Kenyan isolates were identical to those of the J genogroup found mostly in Asia. The findings have implications for biosecurity measures and import regulations for the Kenyan rainbow trout industry.", "gemini": "Virus hoại tử tạo máu truyền nhiễm (IHNV) là tác nhân gây bệnh hoại tử tạo máu truyền nhiễm, một căn bệnh của cá hồi gây ra tổn thất kinh tế lớn. Bệnh xảy ra ở hầu hết các vùng trên thế giới nơi nuôi cá hồi cầu vồng nhưng trước đây chưa từng được báo cáo ở Kenya. Trong nghiên cứu này, cá hồi cầu vồng và cá giống từ hai trang trại ở Quận Nyeri đã được sàng lọc IHNV. Toàn bộ cá giống (n = 4 từ mỗi trang trại) và mẫu thận từ cá giống (n = 15 và n = 6 tương ứng từ hai trang trại) đã được thu thập và bảo quản để xét nghiệm nuôi cấy tế bào hoặc phân tích PCR. Sàng lọc mẫu được thực hiện bằng PCR sau đó là giải trình tự gen glycoprotein của vi-rút. Việc chứng minh vi-rút được thực hiện bằng cách nhân lên trong các tế bào EPC sau đó là xét nghiệm kháng thể huỳnh quang gián tiếp (IFAT). Kết quả cho thấy sự hiện diện của IHNV ở mức độ lưu hành thấp 0,1 và 0,4 đối với hai trang trại. Vi-rút đã được xác nhận bằng cả IFAT và giải trình tự một phần gen G. Phân tích phát sinh loài cho thấy các chủng Kenya giống hệt với các chủng của nhóm gen J chủ yếu được tìm thấy ở Châu Á. Những phát hiện này có ý nghĩa đối với các biện pháp an toàn sinh học và quy định nhập khẩu đối với ngành công nghiệp cá hồi cầu vồng của Kenya."} +{"idx": 1890012, "en": "Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in adults. Basic leucine zipper and W2 domains 2 (BZW2) is a member of the basic-region leucine zipper (bZIP) superfamily of transcription factors. Here, we found that BZW2 expression was substantially increased in both human HCC tissues and cell lines, which was correlated with the clinical progression of patients with HCC. Silence of BZW2 in HCC cells by infecting with the lentivirus for delivering BZW2 shRNA (short hairpin RNA), prohibits cell progression, as determined by the suppressed cell proliferation, clonality, invasion, and increased cell apoptosis. Furthermore, overexpression of BZW2 promotes drug resistance of HCC cells, as shown by the attenuated suppression of cell viability and invasion following rapamycin (RAPA) treatment. Mechanistically, overexpression (or silence) of BZW2 in HCC cells significantly stimulates (or decreases) the activation of the PI3K/AKT/mTOR signaling pathway, which is responsible for HCC progression. Thus, increased BZW2 expression in HCC can induce HCC progression and drug resistance via stimulating the PI3K/AKT/mTOR pathway, which may represent a new therapeutic target for HCC.", "gemini": "Ung thư biểu mô tế bào gan (HCC) là một trong những khối u ác tính phổ biến nhất ở người lớn. Miền leucine zipper cơ bản và W2 (BZW2) là thành viên của siêu họ yếu tố phiên mã leucine zipper vùng cơ bản (bZIP). Ở đây, chúng tôi phát hiện ra rằng biểu hiện BZW2 tăng đáng kể ở cả mô HCC của người và dòng tế bào, có liên quan đến tiến triển lâm sàng của bệnh nhân HCC. Sự im lặng của BZW2 trong các tế bào HCC bằng cách lây nhiễm lentivirus để cung cấp shRNA BZW2 (RNA sợi kẹp ngắn), ngăn cản sự tiến triển của tế bào, được xác định bằng cách ức chế sự tăng sinh tế bào, tính vô tính, xâm lấn và tăng apoptosis tế bào. Hơn nữa, sự biểu hiện quá mức của BZW2 thúc đẩy khả năng kháng thuốc của các tế bào HCC, như được thể hiện bằng việc ức chế giảm khả năng sống và xâm lấn của tế bào sau khi điều trị bằng rapamycin (RAPA). Về mặt cơ chế, sự biểu hiện quá mức (hoặc im lặng) của BZW2 trong các tế bào HCC kích thích đáng kể (hoặc làm giảm) sự hoạt hóa của con đường tín hiệu PI3K/AKT/mTOR, chịu trách nhiệm cho sự tiến triển của HCC. Do đó, sự gia tăng biểu hiện BZW2 ở HCC có thể gây ra sự tiến triển của HCC và kháng thuốc thông qua việc kích thích con đường PI3K/AKT/mTOR, có thể đại diện cho một mục tiêu điều trị mới cho HCC."} +{"idx": 1890013, "en": "Renal cell carcinoma (RCC) is a common urinary system cancer with high morbidity and mortality rate. Clear cell renal cell carcinoma (ccRCC) is a highly aggressive and common type of RCC. More and effective therapeutic targets are badly needed for the treatment of ccRCC. Kinesin family protein (KIF)20B, also named M-phase phosphoprotein 1, was reported as a microtubule-associated, plus-end-directed kinesin. KIF20B was involved in multiple cellular processes such as cytokinesis. Multiple studies indicated the oncogenic role for KIF20B in several types of tumors, including breast cancer and bladder cancer. However, the possible role of KIF20B in the progression of renal carcinoma is still unknown. Herein, our study demonstrated that KIF20B was relatively highly expressed in ccRCC tissues. In addition, KIF20B was inversely related to the clinical features including tumor size and T stage. We further found that inhibition of the KIF20B expression by a specific short hairpin RNA obviously reduces proliferation of ccRCC cells both in vitro and in vivo. Our study reveals the involvement of KIF20B in ccRCC progression. Generally, KIF20B is a promising novel therapeutic for the treatment of clear cell RCC.", "gemini": "Ung thư tế bào thận (RCC) là một bệnh ung thư hệ thống tiết niệu phổ biến với tỷ lệ mắc và tử vong cao. Ung thư tế bào thận tế bào sáng (ccRCC) là một loại RCC rất hung dữ và phổ biến. Rất cần các mục tiêu điều trị hiệu quả hơn cho việc điều trị ccRCC. Protein họ kinesin (KIF)20B, còn được gọi là phosphoprotein pha M-1, được báo cáo là kinesin liên kết với vi ống, hướng về đầu dương. KIF20B tham gia vào nhiều quá trình tế bào như phân chia tế bào chất. Nhiều nghiên cứu chỉ ra vai trò gây ung thư của KIF20B ở một số loại khối u, bao gồm ung thư vú và ung thư bàng quang. Tuy nhiên, vai trò có thể có của KIF20B trong sự tiến triển của ung thư thận vẫn chưa được biết. Trong nghiên cứu này, chúng tôi chứng minh rằng KIF20B được biểu hiện tương đối cao ở các mô ccRCC. Ngoài ra, KIF20B có mối quan hệ nghịch đảo với các đặc điểm lâm sàng bao gồm kích thước khối u và giai đoạn T. Chúng tôi tiếp tục phát hiện ra rằng việc ức chế biểu hiện KIF20B bằng một RNA sợi kẹp ngắn đặc hiệu rõ ràng làm giảm sự tăng sinh của các tế bào ccRCC cả trong ống nghiệm và trong cơ thể sống. Nghiên cứu của chúng tôi tiết lộ sự tham gia của KIF20B vào sự tiến triển của ccRCC. Nhìn chung, KIF20B là một liệu pháp mới đầy hứa hẹn cho việc điều trị RCC tế bào sáng."} +{"idx": 1890014, "en": "An epidemiological study was carried out in Norway in 2015-2018, investigating the development of infection with Piscine myocarditis virus (PMCV) and development of cardiomyopathy syndrome (CMS) in farmed Atlantic salmon. Cohorts from 12 sites were followed and sampled every month or every other month from sea transfer to slaughter. PMCV was detected at all sites and in all sampled cages, and fish in six sites developed clinical CMS. The initial infection happened between 1 and 7 months post-sea transfer, and the median time from infection with PMCV until outbreak of CMS was 6.5 months. Generally, fish from sites with CMS had higher viral titre and a higher prevalence of PMCV, compared to sites that did not develop clinical CMS. The virus persisted until the point of slaughter at most (11 out of 12) of the sites. The detection of PMCV in all sites suggests that PMCV is more widespread than previously known. Screening for PMCV as a tool to monitor impending outbreaks of CMS must be supported by observations of the health status of the fish and risk factors for development of disease.", "gemini": "Một nghiên cứu dịch tễ học đã được thực hiện ở Na Uy vào năm 2015-2018, điều tra sự phát triển của tình trạng nhiễm virus viêm cơ tim ở cá hồi (PMCV) và sự phát triển của hội chứng bệnh cơ tim (CMS) ở cá hồi Đại Tây Dương nuôi. Các nhóm từ 12 địa điểm đã được theo dõi và lấy mẫu hàng tháng hoặc cách tháng một lần từ khi chuyển xuống biển đến khi giết mổ. PMCV được phát hiện tại tất cả các địa điểm và trong tất cả các lồng được lấy mẫu, và cá ở sáu địa điểm đã phát triển CMS lâm sàng. Nhiễm trùng ban đầu xảy ra từ 1 đến 7 tháng sau khi chuyển xuống biển và thời gian trung bình từ khi nhiễm PMCV đến khi bùng phát CMS là 6,5 tháng. Nhìn chung, cá từ các địa điểm có CMS có nồng độ vi-rút cao hơn và tỷ lệ mắc PMCV cao hơn so với các địa điểm không phát triển CMS lâm sàng. Vi-rút vẫn tồn tại cho đến thời điểm giết mổ tại hầu hết (11 trên 12) các địa điểm. Việc phát hiện PMCV tại tất cả các địa điểm cho thấy PMCV phổ biến hơn nhiều so với trước đây. Việc sàng lọc PMCV như một công cụ để theo dõi các đợt bùng phát CMS sắp xảy ra phải được hỗ trợ bằng cách quan sát tình trạng sức khỏe của cá và các yếu tố nguy cơ gây bệnh."} +{"idx": 1890015, "en": "BACKGROUND Patients with chronic kidney disease (CKD) have a severe vitamin D deficiency and increasing epidemiological data suggesting that this deficiency may play a role in overall morbidity and mortality associated with CKD. It is known that vitamin D regulates the immune system, however, in dialysis patients this deficiency and the modulation of proinflammatory cells is unclear. Among these, monocytes arouse interest considering they constitutively express vitamin D receptors. AIM This study aimed the evaluation of monocytic profile in CKD patients according to vitamin D levels. METHODS Patients in hemodialysis (HD) were divided into two groups, regarding vitamin D levels: Group 1, vitamin D <26 ng/ml (n = 15) and Group 2, vitamin D ≥26 ng/ml (n = 18). Whole blood was collected aiming evaluation of (a) monocytic populations through CD14 and CD16 expression, (b) reactive oxygen species (ROS) generation, and (c) apoptosis. RESULTS We observed that in Group 1, when compared to Group 2, there was a significant increase in intermediate monocytes (CD14++ CD16 + ; 34.7 ± 31.6 vs. 12.1 ± 6.3; p = 0.006, respectively) and decrease in classical ones (CD14 ++ CD16 - ; 45.3 ± 31.8 vs. 70.4 ± 25.1; p = 0.017, respectively). There was no difference between groups regarding nonclassical monocytes (CD14 + CD16 ++ ), as well as to apoptosis and to ROS generation. CONCLUSION This study suggests that HD patients with lower vitamin D levels might have an intensified inflammatory outline as intermediate monocytes with an inflammatory pattern are increased in this population, when compared with patients with higher levels of vitamin D.", "gemini": "BỐI CẢNH Bệnh nhân mắc bệnh thận mãn tính (CKD) bị thiếu hụt vitamin D nghiêm trọng và ngày càng có nhiều dữ liệu dịch tễ học cho thấy tình trạng thiếu hụt này có thể đóng vai trò trong tỷ lệ mắc bệnh và tử vong nói chung liên quan đến CKD. Người ta biết rằng vitamin D điều hòa hệ thống miễn dịch, tuy nhiên, ở những bệnh nhân chạy thận nhân tạo, tình trạng thiếu hụt này và sự điều chỉnh các tế bào tiền viêm vẫn chưa rõ ràng. Trong số các tế bào này, các tế bào đơn nhân gây chú ý vì chúng biểu hiện thụ thể vitamin D theo cấu thành. MỤC ĐÍCH Nghiên cứu này nhằm mục đích đánh giá hồ sơ đơn nhân ở bệnh nhân CKD theo nồng độ vitamin D. PHƯƠNG PHÁP Những bệnh nhân đang chạy thận nhân tạo (HD) được chia thành hai nhóm, liên quan đến nồng độ vitamin D: Nhóm 1, vitamin D <26 ng/ml (n = 15) và Nhóm 2, vitamin D ≥26 ng/ml (n = 18). Máu toàn phần được thu thập với mục đích đánh giá (a) quần thể đơn nhân thông qua biểu hiện CD14 và CD16, (b) tạo ra các loài oxy phản ứng (ROS) và (c) chết theo chương trình. KẾT QUẢ Chúng tôi quan sát thấy rằng ở Nhóm 1, khi so sánh với Nhóm 2, có sự gia tăng đáng kể các tế bào đơn nhân trung gian (CD14 ++ CD16 +; 34,7 ± 31,6 so với 12,1 ± 6,3; tương ứng là p = 0,006) và giảm các tế bào cổ điển (CD14 ++ CD16 -; 45,3 ± 31,8 so với 70,4 ± 25,1; tương ứng là p = 0,017). Không có sự khác biệt giữa các nhóm về các tế bào đơn nhân không cổ điển (CD14 + CD16 ++), cũng như về quá trình chết theo chương trình và tạo ra ROS. KẾT LUẬN Nghiên cứu này cho thấy rằng bệnh nhân HD có nồng độ vitamin D thấp hơn có thể có phác đồ viêm mạnh hơn vì các tế bào đơn nhân trung gian có kiểu viêm tăng lên trong quần thể này, khi so sánh với những bệnh nhân có nồng độ vitamin D cao hơn."} +{"idx": 1890016, "en": "The aim of this study is to investigate the effects of long-chain noncoding RNA plasmacytoma variant translocation 1 (PVT1) on the activation of astrocytes and the expression of brain-derived neurotrophic factor (BDNF) in hippocampus tissues of epileptic rats. The epilepsy rat model was induced by intraperitoneal injection of lithium chloride-pilocarpine. Successfully modeled rats were grouped, and their spatial learning and memory, neuronal loss, number of TdT-mediated dUTP nick labeling (TUNEL)-positive cells, and the expression of cleaved-caspase-3, pro-caspase-3, Bax, Bcl-2, GFAP, BDNF, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, axin, and cyclin D1 in hippocampus tissues were evaluated. Increased expression of PVT1 was found in hippocampus tissues of epileptic rats. Silencing of PVT1 improved spatial learning and memory, decreased neuronal loss, decreased the number of TUNEL-positive cell, decreased the expression of cleaved-caspase-3 and Bax while increased pro-caspase-3 and Bcl-2 expression, decreased the expression of GFAP, increased the expression of BDNF, decreased the expression of TNF-α, IL-1β, and IL-6, and decreased the expression of axin and cyclin D1 in hippocampus tissues in epileptic rats. Our study provides evidence that the inhibition of PVT1 may decrease the loss of neurons, inhibit the activation of astrocytes, and increase the expression of BDNF in hippocampus by downregulating the Wnt signaling pathway.", "gemini": "Mục đích của nghiên cứu này là tìm hiểu tác động của RNA không mã hóa chuỗi dài biến đổi chuyển vị u tủy xương (PVT1) lên sự hoạt hóa của tế bào thần kinh đệm và biểu hiện của yếu tố dinh dưỡng thần kinh có nguồn gốc từ não (BDNF) trong mô hồi của chuột động kinh. Mô hình chuột động kinh được gây ra bằng cách tiêm nội phúc mạc lithium clorua-pilocarpin. Những con chuột được tạo mô hình thành công được nhóm lại và khả năng học tập và trí nhớ không gian, mất tế bào thần kinh, số lượng tế bào dương tính với đánh dấu nick dUTP qua trung gian TdT (TUNEL) và biểu hiện của caspase-3 cắt, tiền caspase-3, Bax, Bcl-2, GFAP, BDNF, yếu tố hoại tử khối u-α (TNF-α), interleukin-1β (IL-1β), IL-6, axin và cyclin D1 trong mô hồi được đánh giá. Biểu hiện tăng lên của PVT1 được tìm thấy trong mô hồi của chuột động kinh. Việc làm im lặng PVT1 cải thiện khả năng học tập và trí nhớ không gian, giảm mất tế bào thần kinh, giảm số lượng tế bào dương tính với TUNEL, giảm biểu hiện của caspase-3 cắt và Bax trong khi tăng biểu hiện tiền caspase-3 và Bcl-2, giảm biểu hiện của GFAP, tăng biểu hiện của BDNF, giảm biểu hiện của TNF-α, IL-1β và IL-6, và giảm biểu hiện của axin và cyclin D1 trong mô hồi ở chuột động kinh. Nghiên cứu của chúng tôi cung cấp bằng chứng cho thấy việc ức chế PVT1 có thể làm giảm mất tế bào thần kinh, ức chế hoạt hóa tế bào thần kinh đệm và tăng biểu hiện BDNF trong hồi bằng cách điều chỉnh giảm con đường truyền tín hiệu Wnt."} +{"idx": 1890017, "en": "Coronary artery disease (CAD) is the most common type of cardiovascular disease and becomes a leading cause of death worldwide. Aiming to uncover the underlying molecular features for different types of CAD, we classified 352 CAD cases into three subgroups based on gene expression profiles, which were retrieved from the Gene Expression Omnibus database. Also, these subgroups present different expression patterns and clinical characteristics. To uncover the transcriptomic differences between the subgroups, weighted gene co-expression analysis (WGCNA) was used and identified six subgroup-specific WGCNA modules. Characterization of the WCGNA modules revealed that lipid metabolism pathways, specifically upregulated in subgroup I, might be an indicator of increased severity. Moreover, subgroup II was considered as an early-stage of CAD because of normal-like gene expression patterns. In contrast, the mammalian target of rapamycin signaling pathway was significantly upregulated in subgroup III. Although subgroups II and III did not have a significant prognostic difference, their intrinsic biological characteristics were highly different, suggesting that the transcriptome classification may represent risk factors of both age and the intrinsic biological characteristics. In conclusion, the transcriptome classification of CAD cases revealed that cases from different subgroups may have their unique gene expression patterns, indicating that patients in each subgroup should receive more personalized treatment.", "gemini": "Bệnh động mạch vành (CAD) là loại bệnh tim mạch phổ biến nhất và trở thành nguyên nhân tử vong hàng đầu trên toàn thế giới. Với mục đích khám phá các đặc điểm phân tử cơ bản cho các loại CAD khác nhau, chúng tôi đã phân loại 352 trường hợp CAD thành ba nhóm nhỏ dựa trên hồ sơ biểu hiện gen, được lấy từ cơ sở dữ liệu Gene Expression Omnibus. Ngoài ra, các nhóm nhỏ này thể hiện các kiểu biểu hiện và đặc điểm lâm sàng khác nhau. Để khám phá sự khác biệt về phiên mã giữa các nhóm nhỏ, phân tích đồng biểu hiện gen có trọng số (WGCNA) đã được sử dụng và xác định sáu mô-đun WGCNA đặc hiệu cho nhóm nhỏ. Đặc điểm của các mô-đun WGCNA cho thấy rằng các con đường chuyển hóa lipid, đặc biệt là tăng điều hòa ở nhóm nhỏ I, có thể là một chỉ báo về mức độ nghiêm trọng gia tăng. Hơn nữa, nhóm nhỏ II được coi là giai đoạn đầu của CAD vì các kiểu biểu hiện gen giống như bình thường. Ngược lại, con đường truyền tín hiệu mTOR ở động vật có vú được điều hòa tăng lên đáng kể ở nhóm nhỏ III. Mặc dù các nhóm nhỏ II và III không có sự khác biệt đáng kể về tiên lượng, nhưng các đặc điểm sinh học nội tại của chúng lại rất khác nhau, cho thấy rằng phân loại phiên mã có thể đại diện cho các yếu tố rủi ro của cả tuổi tác và các đặc điểm sinh học nội tại. Tóm lại, phân loại phiên mã các trường hợp CAD cho thấy rằng các trường hợp từ các nhóm nhỏ khác nhau có thể có các kiểu biểu hiện gen độc đáo của riêng chúng, chỉ ra rằng bệnh nhân trong mỗi nhóm nhỏ nên được điều trị theo cách cá nhân hóa hơn."} +{"idx": 1890018, "en": "Lupus nephritis (LN), an autoimmune kidney disease caused by systemic lupus erythematosus (SLE), is the inflammation of the kidney. Although the treatment of LN is still a therapeutic challenge for many practitioners, the present study aims to provide a new insight for the treatment and management. The study aims to explore the effect of A20 on LN in relation to the nuclear factor-kappa B (NF-κB) signaling pathway. MRL/lpr mice were used as the LN mouse model. Next, A20, UCH-L1, and NF-κB expression in LN patients and MRL/lpr mice was determined. A20 was upregulated in podocytes to assess biological functions of A20 in LN. Furthermore, to further investigate the pivotal role of the NF-κB pathway in LN, the NF-κB pathway was blocked in podocytes. Next, UCH-L1 was downregulated in MRL/lpr mice to assess biological functions of UCH-L1 in LN. A20 was downregulated, whereas UCH-L1 was upregulated in LN. Overexpressed A20 declined NF-κB, UCH-L1 expression, and the extent of p65 phosphorylation. A20 overexpression or UCH-L1 inhibition increased expression of synaptoporin and nephrin but decreased desmin expression and ubiquitin accumulation level in podocytes. Moreover, A20 overexpression or UCH-L1 inhibition increased the podocyte number but decreased protein level of cleaved caspase-3, podocyte lesion improvement, decreased foot process width, glomerulus basement membrane, and foot process fusion rate. In addition, urine protein, blood urea nitrogen, serum creatinine, and ds-DNA antibody levels decreased with elevated A20 or depleted UCH-L1. Collectively, it could be concluded that A20 protects against podocyte injury in LN via UCH-L1 by inactivating the NF-κB signaling pathway.", "gemini": "Viêm thận lupus (LN), một bệnh thận tự miễn do lupus ban đỏ hệ thống (SLE) gây ra, là tình trạng viêm của thận. Mặc dù việc điều trị LN vẫn là một thách thức về mặt điều trị đối với nhiều bác sĩ, nghiên cứu hiện tại nhằm cung cấp một hiểu biết mới về việc điều trị và quản lý. Nghiên cứu này nhằm mục đích khám phá tác dụng của A20 đối với LN liên quan đến con đường truyền tín hiệu nhân tố hạt nhân kappa B (NF-κB). Chuột MRL/lpr được sử dụng làm mô hình chuột LN. Tiếp theo, biểu hiện của A20, UCH-L1 và NF-κB ở bệnh nhân LN và chuột MRL/lpr đã được xác định. A20 được tăng lên trong tế bào chân đế để đánh giá các chức năng sinh học của A20 trong LN. Hơn nữa, để nghiên cứu sâu hơn về vai trò quan trọng của con đường NF-κB trong LN, con đường NF-κB đã bị chặn trong các tế bào chân đế. Tiếp theo, UCH-L1 đã bị giảm trong chuột MRL/lpr để đánh giá các chức năng sinh học của UCH-L1 trong LN. A20 bị giảm, trong khi UCH-L1 tăng lên trong LN. A20 biểu hiện quá mức làm giảm NF-κB, biểu hiện UCH-L1 và mức độ phosphoryl hóa p65. Sự biểu hiện quá mức của A20 hoặc sự ức chế UCH-L1 làm tăng biểu hiện của synaptoporin và nephrin nhưng làm giảm biểu hiện desmin và mức độ tích tụ ubiquitin trong các tế bào chân đế. Hơn nữa, sự biểu hiện quá mức của A20 hoặc sự ức chế UCH-L1 làm tăng số lượng tế bào chân đế nhưng làm giảm mức protein của caspase-3 bị cắt, cải thiện tổn thương tế bào chân đế, giảm chiều rộng của chân tế bào, màng đáy cầu thận và tỷ lệ hợp nhất của chân tế bào. Ngoài ra, lượng protein trong nước tiểu, nitơ urê máu, creatinin huyết thanh và kháng thể ds-DNA giảm khi A20 tăng hoặc UCH-L1 cạn kiệt. Kết hợp lại, có thể kết luận rằng A20 bảo vệ chống lại tổn thương tế bào chân đế trong LN thông qua UCH-L1 bằng cách bất hoạt con đường truyền tín hiệu NF-κB."} +{"idx": 1890019, "en": "Adipose tissue-derived mesenchymal stem cells (ASCs) from livestock are valuable resources for animal reproduction and veterinary therapeutics. Previous studies have shown that hypoxic conditions were beneficial in maintaining the physiological activities of ASCs. However, the effects of hypoxia on buffalo ASCs (bASCs) remain unclear. In this study, the effects of hypoxia on proliferation, stemness, and reprogramming into induced pluripotent stem cells (iPSCs) of bASCs were examined. The results showed that the hypoxic culture conditions (5% oxygen) enhanced the proliferation and colony formation of bASCs. The expression levels of proliferation-related genes, and secretion of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) were significantly enhanced in hypoxia. Hypoxic culture conditions activated hypoxia-inducible factor-1α (HIF-1α), thereby contributing to the secretion of bFGF and VEGF, which in turn enhanced the expression of HIF-1α and promoted the proliferation of bASCs. Furthermore, in hypoxic culture conditions, bASCs exhibited the main characteristics of mesenchymal stem cells, and the expression levels of the pluripotent markers OCT4, NANOG, C-MYC, and the differentiation capacity of bASCs were significantly enhanced. Finally, bASCs were more efficiently and easily reprogrammed into iPSCs in hypoxic culture conditions and these iPSCs exhibited some characteristics of naïve pluripotent stem cells. These findings provide the theoretical guidance for elucidating the detailed mechanism of hypoxia on physiological activities of bASCs including proliferation, stemness maintenance, and reprogramming.", "gemini": "Các tế bào gốc trung mô có nguồn gốc từ mô mỡ (ASCs) từ gia súc là nguồn tài nguyên có giá trị cho việc sinh sản động vật và điều trị thú y. Các nghiên cứu trước đây đã chỉ ra rằng các điều kiện thiếu oxy có lợi trong việc duy trì các hoạt động sinh lý của ASCs. Tuy nhiên, những ảnh hưởng của tình trạng thiếu oxy đối với ASCs của trâu (bASCs) vẫn chưa được làm rõ. Trong nghiên cứu này, các tác động của tình trạng thiếu oxy đối với sự tăng sinh, tính gốc và tái lập trình thành các tế bào gốc đa năng cảm ứng (iPSCs) của bASCs đã được kiểm tra. Kết quả cho thấy các điều kiện nuôi cấy thiếu oxy (5% oxy) làm tăng sự tăng sinh và hình thành khuẩn lạc của bASCs. Mức độ biểu hiện của các gen liên quan đến sự tăng sinh và sự tiết ra yếu tố tăng trưởng nguyên bào sợi cơ bản (bFGF) và yếu tố tăng trưởng nội mô mạch máu (VEGF) được tăng cường đáng kể trong tình trạng thiếu oxy. Các điều kiện nuôi cấy thiếu oxy đã kích hoạt yếu tố gây thiếu oxy-1α (HIF-1α), do đó góp phần vào quá trình tiết ra bFGF và VEGF, từ đó tăng cường biểu hiện của HIF-1α và thúc đẩy sự tăng sinh của bASCs. Hơn nữa, trong điều kiện nuôi cấy thiếu oxy, bASCs biểu hiện các đặc điểm chính của tế bào gốc trung mô và mức độ biểu hiện của các dấu hiệu đa năng OCT4, NANOG, C-MYC và khả năng biệt hóa của bASCs được tăng cường đáng kể. Cuối cùng, bASCs được tái lập trình hiệu quả và dễ dàng hơn thành các iPSC trong điều kiện nuôi cấy thiếu oxy và các iPSC này biểu hiện một số đặc điểm của các tế bào gốc đa năng ngây thơ. Những phát hiện này cung cấp hướng dẫn lý thuyết để làm sáng tỏ cơ chế chi tiết của tình trạng thiếu oxy đối với các hoạt động sinh lý của bASCs bao gồm sự tăng sinh, duy trì tính gốc và tái lập trình."} +{"idx": 1890020, "en": "OBJECTIVES The cationic biopolymer chitosan (CH) has emerged as a promising candidate adjuvant due to its safety profile and immunostimulatory properties. The presence of endotoxin contamination in biomaterials is generally underappreciated and can generate misleading results. It is important to establish a convenient methodology to obtain large amounts of high quality chitosan nanoparticles for biomedical applications. METHODS We developed an easy method to generate endotoxin-free chitosan and assessed its purity using the Limulus amebocyte lysate assay and by measuring dendritic cell activation. KEY FINDINGS Purified chitosan-based formulations alone failed to induce production of the proinflammatory cytokines tumour necrosis factor alpha (TNF-α) and interleukin (IL)-6 in bone marrow-derived dendritic cells (BMDCs) generated from C57BL/6 mice, while maintaining its ability to promote IL-1β secretion in combination with the Toll-like receptor (TLR)-9 agonist, CpG. Moreover, BMDCs from C3H/HeN and TLR4-deficient mice, C3H/HeJ were stimulated with endotoxin-free chitosan-based formulations and no differences were observed in IL-6 and IL-1β secretion, excluding the involvement of TLR-4 in the immunomodulatory effects of chitosan. CONCLUSIONS The developed method provides simple guidelines for the production of endotoxin-free chitosan, ideal for biomedical applications.", "gemini": "MỤC TIÊU Biopolymer cation chitosan (CH) đã nổi lên như một tá dược ứng cử viên đầy hứa hẹn do hồ sơ an toàn và các đặc tính kích thích miễn dịch của nó. Sự hiện diện của nhiễm bẩn nội độc tố trong các vật liệu sinh học thường không được đánh giá đúng mức và có thể tạo ra kết quả gây hiểu lầm. Điều quan trọng là phải thiết lập một phương pháp thuận tiện để thu được một lượng lớn các hạt nano chitosan chất lượng cao cho các ứng dụng y sinh. PHƯƠNG PHÁP Chúng tôi đã phát triển một phương pháp dễ dàng để tạo ra chitosan không chứa nội độc tố và đánh giá độ tinh khiết của nó bằng cách sử dụng xét nghiệm giải bào amip Limulus và bằng cách đo hoạt hóa tế bào樹狀. NHỮNG PHÁT HIỆN CHÍNH Các chế phẩm dựa trên chitosan tinh khiết không gây ra sản xuất các cytokine tiền viêm yếu tố hoại tử khối u alpha (TNF-α) và interleukin (IL)-6 trong các tế bào樹狀 có nguồn gốc từ tủy xương (BMDC) được tạo ra từ chuột C57BL/6, trong khi vẫn duy trì khả năng thúc đẩy tiết IL-1β kết hợp với chất chủ vận thụ thể giống Toll (TLR)-9, CpG. Hơn nữa, BMDC từ chuột C3H/HeN và chuột thiếu TLR4, C3H/HeJ đã được kích thích bằng các chế phẩm dựa trên chitosan không chứa nội độc tố và không có sự khác biệt nào được quan sát thấy trong quá trình tiết IL-6 và IL-1β, loại trừ sự tham gia của TLR-4 trong các tác dụng điều hòa miễn dịch của chitosan. KẾT LUẬN Phương pháp được phát triển cung cấp các hướng dẫn đơn giản để sản xuất chitosan không chứa nội độc tố, lý tưởng cho các ứng dụng y sinh."} +{"idx": 1890021, "en": "BACKGROUND Nickel is one of the most common contact allergens. Despite EU regulations concerning the maximum permissible release of nickel from metal objects, nickel release at inappropriately high levels from metal objects has been detected in various studies. OBJECTIVES To screen current nickel release from metal tools in the German hairdressing trade. METHODS Two hundred and twenty-nine metal tools from the German hairdressing trade were tested with the dimethylglyoxime (DMG) test for nickel release. Additionally, an employee survey was conducted to collect data about the metal tools. RESULTS The DMG test showed that 21 of 229 metal tools (9.2%) released nickel. Nickel release was detected in 8 of 45 hair clips (17.8%), 2 of 27 tail combs (7.4%), and 11 of 17 tweezers (64.7%). CONCLUSION It must be assumed that hairdressers in Germany have prolonged skin contact with nickel-releasing metal tools. This should prompt further testing for nickel release from metal tools, and the development of regulations to eliminate nickel-releasing metal tools in the (German) hairdressing trade.", "gemini": "BỐI CẢNH Niken là một trong những chất gây dị ứng tiếp xúc phổ biến nhất. Mặc dù có các quy định của EU liên quan đến việc giải phóng niken tối đa cho phép từ các vật dụng bằng kim loại, việc giải phóng niken ở mức cao không phù hợp từ các vật dụng bằng kim loại đã được phát hiện trong nhiều nghiên cứu khác nhau. MỤC TIÊU Kiểm tra việc giải phóng niken hiện tại từ các dụng cụ bằng kim loại trong ngành làm tóc của Đức. PHƯƠNG PHÁP Hai trăm hai mươi chín dụng cụ bằng kim loại từ ngành làm tóc của Đức đã được thử nghiệm bằng thử nghiệm dimethylglyoxime (DMG) để giải phóng niken. Ngoài ra, một cuộc khảo sát nhân viên đã được tiến hành để thu thập dữ liệu về các dụng cụ bằng kim loại. KẾT QUẢ Thử nghiệm DMG cho thấy 21/229 dụng cụ bằng kim loại (9,2%) giải phóng niken. Việc giải phóng niken được phát hiện ở 8/45 kẹp tóc (17,8%), 2/27 lược đuôi (7,4%) và 11/17 nhíp (64,7%). KẾT LUẬN Người ta phải cho rằng những người làm tóc ở Đức tiếp xúc lâu dài với các dụng cụ bằng kim loại giải phóng niken. Điều này sẽ thúc đẩy việc thử nghiệm thêm về việc giải phóng niken từ các dụng cụ bằng kim loại và việc xây dựng các quy định để loại bỏ các dụng cụ bằng kim loại giải phóng niken trong ngành làm tóc (Đức)."} +{"idx": 1890022, "en": "Wnt1-inducible signaling protein 1 (WISP1) is a matricellular protein and downstream target of Wnt/β-catenin signaling. This study sought to determine the role of WISP1 in glucose metabolism and chemoresistance in laryngeal squamous cell carcinoma. WISP1 expression was silenced or upregulated in Hep-2 cells by the transfection of WISP1 siRNA or AdWISP1 vector. Ectopic WISP1 expression regulated glucose uptake and lactate production in Hep-2 cells. Subsequently, the expression of glucose transporter 1 (GLUT1) was significantly modulated by WISP1. Furthermore, WISP1 increased cell survival rates, diminished cell death rates, and suppressed ataxia-telangiectasia-mutated (ATM)-mediated DNA damage response pathway in cancer cells treated with cisplatin through GLUT1. WISP1 also promoted cancer cell tumorigenicity and growth in mice implanted with Hep-2 cells. Additionally, WISP1 activated the YAP1/TEAD1 pathway that consequently contributed to the regulation of GLUT1 expression. In summary, WISP1 regulated glucose metabolism and cisplatin resistance in laryngeal cancer by regulating GLUT1 expression. WISP1 may be used as a potential therapeutic target for laryngeal cancer.", "gemini": "Protein 1 có thể gây ra tín hiệu Wnt1 (WISP1) là một protein ngoại bào và là mục tiêu hạ nguồn của tín hiệu Wnt/β-catenin. Nghiên cứu này tìm cách xác định vai trò của WISP1 trong quá trình chuyển hóa glucose và khả năng kháng hóa chất ở ung thư biểu mô tế bào vảy thanh quản. Biểu hiện WISP1 đã bị làm im lặng hoặc được điều chỉnh tăng lên ở các tế bào Hep-2 bằng cách chuyển gen siRNA WISP1 hoặc vectơ AdWISP1. Biểu hiện WISP1 ngoại lai điều chỉnh quá trình hấp thu glucose và sản xuất lactate ở các tế bào Hep-2. Sau đó, biểu hiện của chất vận chuyển glucose 1 (GLUT1) bị WISP1 điều chỉnh đáng kể. Hơn nữa, WISP1 làm tăng tỷ lệ tế bào sống sót, giảm tỷ lệ tế bào chết và ức chế con đường phản ứng với tổn thương DNA do đột biến ataxia-telangiectasia (ATM) ở các tế bào ung thư được điều trị bằng cisplatin thông qua GLUT1. WISP1 cũng thúc đẩy khả năng gây ung thư và sự phát triển của tế bào ung thư ở những con chuột được cấy ghép tế bào Hep-2. Ngoài ra, WISP1 kích hoạt con đường YAP1/TEAD1, do đó góp phần điều chỉnh biểu hiện GLUT1. Tóm lại, WISP1 điều chỉnh quá trình chuyển hóa glucose và khả năng kháng cisplatin ở ung thư thanh quản bằng cách điều chỉnh biểu hiện GLUT1. WISP1 có thể được sử dụng như một mục tiêu điều trị tiềm năng cho bệnh ung thư thanh quản."} +{"idx": 1890023, "en": "MicroRNAs (miRNAs) participate in the pathological process of liver ischemia/reperfusion (I/R) injury. MiR-449b-5p is the target miRNA of high mobility group box 1 (HMGB1). Its role and molecular mechanism in liver I/R injury remain unidentified. In this study, we found a protective effect of miR-449b-5p against hepatic I/R injury. HMGB1 expression significantly increased, whereas miR-449b-5p dramatically decreased in patients after liver transplant and in L02 cells exposed to hypoxia/reoxygenation (H/R). A dual-luciferase reporter assay confirmed the direct interaction between miR-449b-5p and the 3' untranslated region of HMGB1 messenger RNA. We also found that overexpression of miR-449b-5p significantly promoted cell viability and inhibited cell apoptosis of L02 cells exposed to H/R. Moreover, miR-449b-5p repressed HMGB1 protein expression and nuclear factor-κB (NF-κB) pathway activation in these L02 cells. In an in vivo rat model of hepatic I/R injury, overexpression of miR-449b-5p significantly decreased alanine aminotransferase and aspartate aminotransferase and inhibited the HMGB1/NF-κB pathway. Our study thus suggests that miR-449b-5p alleviated hepatic I/R injury by targeting HMGB1 and deactivating the NF-κB pathway, which may provide a novel and promising therapeutic target for hepatic I/R injury.", "gemini": "MicroRNA (miRNA) tham gia vào quá trình bệnh lý của tổn thương thiếu máu cục bộ/tái tưới máu (I/R) ở gan. MiR-449b-5p là miRNA mục tiêu của hộp nhóm vận động cao 1 (HMGB1). Vai trò và cơ chế phân tử của nó trong tổn thương gan I/R vẫn chưa được xác định. Trong nghiên cứu này, chúng tôi đã tìm thấy tác dụng bảo vệ của miR-449b-5p chống lại tổn thương gan I/R. Biểu hiện HMGB1 tăng đáng kể, trong khi miR-449b-5p giảm đáng kể ở những bệnh nhân sau ghép gan và ở các tế bào L02 tiếp xúc với tình trạng thiếu oxy/tái oxy hóa (H/R). Thử nghiệm phóng viên luciferase kép xác nhận sự tương tác trực tiếp giữa miR-449b-5p và vùng 3' không được dịch mã của RNA thông tin HMGB1. Chúng tôi cũng phát hiện ra rằng việc biểu hiện quá mức miR-449b-5p thúc đẩy đáng kể khả năng sống của tế bào và ức chế quá trình apoptosis của tế bào L02 tiếp xúc với H/R. Hơn nữa, miR-449b-5p ức chế biểu hiện protein HMGB1 và kích hoạt con đường nhân tố hạt nhân-κB (NF-κB) ở các tế bào L02 này. Trong một mô hình chuột sống về tổn thương gan I/R, việc biểu hiện quá mức miR-449b-5p làm giảm đáng kể alanine aminotransferase và aspartate aminotransferase và ức chế con đường HMGB1/NF-κB. Do đó, nghiên cứu của chúng tôi cho thấy miR-449b-5p làm giảm tổn thương gan I/R bằng cách nhắm mục tiêu vào HMGB1 và vô hiệu hóa con đường NF-κB, điều này có thể cung cấp một mục tiêu điều trị mới và đầy hứa hẹn cho tổn thương gan I/R."} +{"idx": 1890024, "en": "NESFATIN-1 acts as a neuroendocrine hormone to suppress gonadotropin secretion in the female goldfish and to prevent germinal vesicle breakdown of oocytes in the zebrafish. However, the expression and function of NESFATIN-1 in meiotic maturation and development of porcine oocytes remains elusive. Genomic structure of porcine NESFATIN-1 precursor nucleobindin 2 (NUCB2) is first characterized in detail and an evolutionally closer relationship of NESFATIN-1 between pig and rat is shown by phylogenetic analysis of multiple species. Additionally, immunofluorescence analysis revealed that NESFATIN-1 is predominantly expressed and localizes on the membrane of both theca cells and granulosa cells, but not expressed in oocytes. Real-time quantitative polymerase chain reaction showed that the abundance of NESFATIN-1 transcripts in granulosa cells progressively decreases during the developmental transition from small follicles to large follicles. Correspondingly, NESFATIN-1 could significantly enhance both the cleavage and blastocyst rate of parthenogenetically activated oocytes from small follicles (p < 0.05), whereas it did not affect meiotic maturation and development of oocytes from large follicles. Interestingly, we found that NESFATIN-1 significantly improves meiotic maturation of oocytes cultured in chemically defined medium in the absence of pyruvate compared with the control group (p < 0.05), suggesting that the NESFATIN-1 as a substitute for pyruvate exerts beneficial effects on porcine oocyte maturation. In conclusion, these results demonstrate that NESFATIN-1 facilitates both meiotic maturation and development of porcine oocytes.", "gemini": "NESFATIN-1 hoạt động như một hormon thần kinh nội tiết để ức chế tiết gonadotropin ở cá vàng cái và ngăn chặn sự phân hủy nang noãn nguyên ở cá ngựa vằn. Tuy nhiên, biểu hiện và chức năng của NESFATIN-1 trong quá trình trưởng thành và phát triển của noãn lợn vẫn chưa được biết rõ. Cấu trúc bộ gen của tiền chất NESFATIN-1 ở lợn là nucleobindin 2 (NUCB2) được đặc trưng chi tiết lần đầu tiên và mối quan hệ tiến hóa gần hơn của NESFATIN-1 giữa lợn và chuột được thể hiện bằng phân tích phả hệ của nhiều loài. Ngoài ra, phân tích miễn dịch huỳnh quang cho thấy NESFATIN-1 chủ yếu được biểu hiện và định vị trên màng của cả tế bào theca và tế bào hạt, nhưng không được biểu hiện trong noãn. Phản ứng chuỗi polymerase định lượng thời gian thực cho thấy hàm lượng bản sao NESFATIN-1 trong tế bào hạt giảm dần trong quá trình chuyển đổi phát triển từ nang nhỏ sang nang lớn. Tương ứng, NESFATIN-1 có thể làm tăng đáng kể cả tỷ lệ phân cắt và tỷ lệ phôi nang của noãn được kích hoạt đơn tính từ nang nhỏ (p <0,05), trong khi nó không ảnh hưởng đến quá trình trưởng thành và phát triển của noãn từ nang lớn. Điều thú vị là chúng tôi thấy rằng NESFATIN-1 cải thiện đáng kể quá trình trưởng thành của noãn được nuôi trong môi trường được xác định về mặt hóa học khi không có pyruvat so với nhóm đối chứng (p <0,05), điều này cho thấy NESFATIN-1 như một chất thay thế cho pyruvat có tác dụng có lợi đối với sự trưởng thành của noãn lợn. Tóm lại, những kết quả này chứng minh rằng NESFATIN-1 tạo điều kiện thuận lợi cho cả quá trình trưởng thành và phát triển của noãn lợn."} +{"idx": 1890025, "en": "K+ channels of the alveolar epithelium control the driving force acting on the ionic and solvent flow through the cell membrane contributing to the maintenance of cell volume and the constitution of epithelial lining fluid. In the present work, we analyze the effect of the Cl- channel inhibitors: (4-[(2-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-inden-5-yl)oxy] butanoic acid (DCPIB) and 9-anthracenecarboxylic acid (9-AC) on the total current in a type II pneumocytes (A549 cell line) model by patch clamp, immunocytochemical, and gene knockdown techniques. We noted that DCPIB and 9-AC promote the activation of K conductance. In fact, they significantly increase the intensity of the current and shift its reversal potential to values more negative than the control. By silencing outward rectifier channel in its anoctamin 6 portion, we excluded a direct involvement of Cl- ions in modulation of IK and, by means of functional tests with its specific inhibitor spadin, we identified the TREK-1 channel as the presumable target of both drugs. As the activity of TREK-1 has a key role for the correct functioning of the alveolar epithelium, the identification of DCPIB and 9-AC molecules as its activators suggests their possible use to build new pharmacological tools for the modulation of this channel.", "gemini": "Các kênh K+ của biểu mô phế nang kiểm soát lực đẩy tác động lên dòng ion và dung môi qua màng tế bào góp phần duy trì thể tích tế bào và cấu thành dịch lót biểu mô. Trong công trình này, chúng tôi phân tích tác dụng của các chất ức chế kênh Cl-: (4-[(2-butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-inden-5-yl)oxy] axit butanoic (DCPIB) và axit 9-anthracenecarboxylic (9-AC) trên tổng dòng điện trong mô hình tế bào phổi loại II (dòng tế bào A549) bằng kỹ thuật kẹp bản vá, miễn dịch tế bào hóa học và hạ gục gen. Chúng tôi nhận thấy rằng DCPIB và 9-AC thúc đẩy sự hoạt hóa của độ dẫn K. Trên thực tế, chúng làm tăng đáng kể cường độ dòng điện và dịch chuyển điện thế đảo ngược của nó đến các giá trị âm hơn so với đối chứng. Bằng cách làm im lặng kênh chỉnh lưu hướng ra ngoài ở phần anoctamin 6 của nó, chúng tôi đã loại trừ sự tham gia trực tiếp của các ion Cl- trong điều chế IK và thông qua các xét nghiệm chức năng với chất ức chế đặc hiệu của nó là spadin, chúng tôi xác định kênh TREK-1 là mục tiêu có thể xảy ra của cả hai loại thuốc. Vì hoạt động của TREK-1 có vai trò quan trọng đối với chức năng bình thường của biểu mô phế nang, nên việc xác định các phân tử DCPIB và 9-AC là chất hoạt hóa của nó gợi ý khả năng sử dụng của chúng để xây dựng các công cụ dược lý mới để điều chế kênh này."} +{"idx": 1890026, "en": "Epidermal growth factor receptor (EGFR) is a central transmitter of mitogenic signals in epithelial cells; enhanced EGFR activity is observed in many tumors of epithelial origin. S100A6 is a small calcium-binding protein, characteristic mainly of epithelial cells and fibroblasts, strongly implicated in cell proliferation and upregulated in tumors. In this study, using biochemical assays along with immunohistochemical and immunocytochemical analysis of organotypic and standard cultures of HaCaT keratinocytes with S100A6 overexpression or knock-down, we have examined the effect of S100A6 on EGFR activity and downstream signaling. We found that HaCaT cells overexpressing S100A6 had enhanced EGFR, phospho EGFR, and phospho extracellular signal-regulated kinase 1/2 (pERK1/2) staining intensity and level coupled to higher signal transducer and activator of transcription 3 (STAT3) activity. Conversely, S100A6 knockdown cells had impaired EGFR signaling that could be enhanced by addition of recombinant S100A6 to the culture media. Altogether the results show that S100A6 may exert its proproliferative effects through activating EGFR.", "gemini": " thụ thể yếu tố tăng trưởng biểu bì (EGFR) là một chất truyền tín hiệu trung tâm của các tín hiệu sinh nguyên trong các tế bào biểu mô; Hoạt động EGFR tăng cường được quan sát thấy ở nhiều khối u có nguồn gốc biểu mô. S100A6 là một protein liên kết canxi nhỏ, đặc trưng chủ yếu của các tế bào biểu mô và nguyên bào sợi, có liên quan chặt chẽ đến sự tăng sinh tế bào và tăng lên trong các khối u. Trong nghiên cứu này, sử dụng các xét nghiệm sinh hóa cùng với phân tích miễn dịch mô học và miễn dịch tế bào hóa học của các nuôi cấy chuẩn và nuôi cấy kiểu cơ quan của tế bào sừng HaCaT với sự biểu hiện quá mức hoặc hạ gục S100A6, chúng tôi đã kiểm tra tác dụng của S100A6 đối với hoạt động của EGFR và tín hiệu hạ nguồn. Chúng tôi thấy rằng các tế bào HaCaT biểu hiện quá mức S100A6 có cường độ nhuộm EGFR, phospho EGFR và phospho extracelluar signal-regulated kinase 1/2 (pERK1/2) tăng lên và mức độ kết hợp với hoạt động của chất truyền tín hiệu và hoạt hóa phiên mã 3 (STAT3) cao hơn. Ngược lại, các tế bào hạ gục S100A6 có tín hiệu EGFR bị suy yếu, có thể được tăng cường bằng cách thêm S100A6 tái tổ hợp vào môi trường nuôi cấy. Nhìn chung, kết quả cho thấy S100A6 có thể phát huy tác dụng thúc đẩy tăng sinh của mình thông qua việc kích hoạt EGFR."} +{"idx": 1890027, "en": "Long noncoding RNA (lncRNA) mortal obligate RNA transcript (MORT) was downregulated many types of cancer tissues, while its functionality in cancer biology is unclear. In the present study, we systemically investigated the involvement of lncRNA MORT in oral squamous cell carcinoma (OSCC). In the present study, we found that lncRNA MORT was downregulated, while rho-associated coiled-coil containing protein kinase 1 (ROCK1) messenger RNA was upregulated in cancer tissues than in adjacent healthy tissues of OSCC patients. In addition, expression levels of lncRNA MORT and ROCK1 were inversely correlated in both tumor tissues and healthy tissues. Follow-up study showed that low MORT level was significantly correlated with poor survival. Overexpression of lncRNA MORT inhibited the proliferation of OSCC cells and downregulated ROCK1. ROCK1 overexpression led to significantly promoted cell proliferation but showed no significant effect on MORT expression. In addition, ROCK1 overexpression attenuated the inhibitory effects of lncRNA MORT overexpression on the proliferation of OSCC cells. Therefore, lncRNA MORT overexpression may inhibit cancer cell proliferation in OSCC cells by downregulating ROCK1.", "gemini": "RNA dài không mã hóa (lncRNA) bản phiên mã RNA bắt buộc tử vong (MORT) bị giảm điều hòa ở nhiều loại mô ung thư, trong khi chức năng của nó trong sinh học ung thư vẫn chưa rõ ràng. Trong nghiên cứu hiện tại, chúng tôi đã nghiên cứu một cách có hệ thống sự tham gia của lncRNA MORT trong ung thư biểu mô tế bào vảy miệng (OSCC). Trong nghiên cứu hiện tại, chúng tôi thấy rằng lncRNA MORT bị giảm điều hòa, trong khi RNA thông tin kinase 1 chứa chuỗi xoắn ốc liên kết với rho (ROCK1) được tăng điều hòa ở các mô ung thư so với các mô lành kề của bệnh nhân OSCC. Ngoài ra, mức độ biểu hiện của lncRNA MORT và ROCK1 có mối tương quan nghịch trong cả mô khối u và mô lành. Nghiên cứu tiếp theo cho thấy mức MORT thấp có liên quan đáng kể đến khả năng sống sót kém. Sự biểu hiện quá mức của lncRNA MORT ức chế sự gia tăng tế bào OSCC và làm giảm điều hòa ROCK1. Sự biểu hiện quá mức của ROCK1 dẫn đến sự gia tăng đáng kể sự gia tăng tế bào nhưng không cho thấy tác dụng đáng kể nào đối với biểu hiện của MORT. Ngoài ra, sự biểu hiện quá mức của ROCK1 làm giảm các tác dụng ức chế của sự biểu hiện quá mức của lncRNA MORT đối với sự gia tăng tế bào OSCC. Do đó, sự biểu hiện quá mức của lncRNA MORT có thể ức chế sự gia tăng tế bào ung thư ở các tế bào OSCC bằng cách làm giảm điều hòa ROCK1."} +{"idx": 1890028, "en": "The mismatch between stiffness of the femoral dense stem and host bone causes complications to patients, such as aseptic loosening and bone resorption. Three-dimensional finite-element models of homogeneous porous (HGP) and functionally graded porous (FGP) stems incorporating body-centered cubic (BCC) structures are proposed in this article as an alternative to the dense stems. The relationship between the porosity and strut thickness of the BCC structure was developed to construct the finite-element models. Three levels of porosities (20%, 50%, and 80%) were modeled in HGP and FGP stems. The porosity of the stems was decreased distally according to the sigmoid function (n = 0.1, n = 1 and n = 10) with 3 grading exponents. The results showed that FGP stems transferred 120%-170% higher stresses to the femur (Gruen zone 7) as compared to the solid stem. Conversely, the stresses in HGP and FGP stems were 12%-34% lower than the dense stem. The highest micromotions (105-147 µm) were observed for stems of 80% overall porosity, and the lowest (42-46 µm) was for stems of 20% overall porosity. Finally, FGP stems with a grading exponent of n = 10 resulted in an 11%-28% reduction in micromotions.", "gemini": "Sự không phù hợp giữa độ cứng của thân xương đùi đặc và xương chủ gây ra các biến chứng cho bệnh nhân, chẳng hạn như nới lỏng vô trùng và tiêu xương. Các mô hình phần tử hữu hạn ba chiều của thân xốp đồng nhất (HGP) và thân xốp phân cấp chức năng (FGP) kết hợp các cấu trúc lập phương tâm khối (BCC) được đề xuất trong bài viết này như một giải pháp thay thế cho thân đặc. Mối quan hệ giữa độ xốp và độ dày thanh chống của cấu trúc BCC đã được phát triển để xây dựng các mô hình phần tử hữu hạn. Ba mức độ xốp (20%, 50% và 80%) đã được mô hình hóa trong thân HGP và FGP. Độ xốp của thân giảm dần theo chức năng sigmoid (n = 0,1, n = 1 và n = 10) với 3 số mũ phân loại. Kết quả cho thấy thân FGP truyền cao hơn 120% -170% ứng suất lên xương đùi (vùng Gruen 7) so với thân rắn. Ngược lại, ứng suất trong thân HGP và FGP thấp hơn 12% -34% so với thân đặc. Các vi chuyển động cao nhất (105-147 µm) được quan sát thấy đối với các thân có độ xốp tổng thể 80% và thấp nhất (42-46 µm) đối với các thân có độ xốp tổng thể 20%. Cuối cùng, thân FGP có số mũ phân loại n = 10 dẫn đến giảm 11% -28% vi chuyển động."} +{"idx": 1890029, "en": "AIMS A dramatic increase in newborn infants with neonatal abstinence syndrome has been observed and these neonates are frequently treated with complex methadone dosing schemes to control their withdrawal symptoms. Despite its abundant use, hardly any data on the pharmacokinetics (PK) of methadone is available in preterm neonates. Therefore we investigated developmental PK of methadone and evaluated current dosing strategies and possible simplification in this vulnerable population. METHODS A single-centre open-label prospective study was performed to collect PK data after a single oral dose of methadone in preterm neonates. A population PK model was built to characterize developmental PK of (R)- and (S)-methadone. Model-based simulations were performed to identify a simplified dosing strategy to reach and maintain target methadone exposure. RESULTS A total of 121 methadone concentrations were collected from 31 preterm neonates. A one-compartment model with first order absorption and elimination kinetics best described PK data for (R)- and (S)-methadone. Clearance increases with advancing gestational age and differs between R- and S-enantiomer, being slightly higher for the former (0.244 vs 0.167 L/h). Preterm neonates reached target exposure after 48 hours with currently used dosing schedules. Output from simulations revealed that target exposures can be achieved with a simplified dosing strategy during the first 4 days of treatment. CONCLUSION Methadone clearance in preterm neonates increases with advancing gestational age and its disposition is influenced by its chirality. Simulations that account for developmental PK changes indicate a shorter methadone dosing strategy can maintain target exposure to control withdrawal symptoms.", "gemini": "MỤC TIÊU Người ta đã quan sát thấy sự gia tăng đáng kể trẻ sơ sinh mắc hội chứng cai nghiện ở trẻ sơ sinh và những trẻ sơ sinh này thường được điều trị bằng các chế độ dùng methadone phức tạp để kiểm soát các triệu chứng cai nghiện của chúng. Mặc dù được sử dụng rất nhiều, nhưng hầu như không có dữ liệu nào về dược động học (PK) của methadone ở trẻ sơ sinh non tháng. Do đó, chúng tôi đã nghiên cứu PK phát triển của methadone và đánh giá các chiến lược dùng thuốc hiện tại và khả năng đơn giản hóa ở nhóm đối tượng dễ bị tổn thương này. PHƯƠNG PHÁP Một nghiên cứu triển vọng nhãn mở đơn trung tâm đã được thực hiện để thu thập dữ liệu PK sau một liều methadone uống duy nhất ở trẻ sơ sinh non tháng. Một mô hình PK của quần thể đã được xây dựng để mô tả đặc điểm PK phát triển của (R) - và (S) -methadone. Các mô phỏng dựa trên mô hình đã được thực hiện để xác định một chiến lược dùng thuốc đơn giản hóa để đạt được và duy trì phơi nhiễm methadone mục tiêu. KẾT QUẢ Tổng cộng 121 nồng độ methadone đã được thu thập từ 31 trẻ sơ sinh non tháng. Một mô hình một ngăn với động học hấp thu và đào thải bậc nhất mô tả tốt nhất dữ liệu PK cho (R) - và (S) -methadone. Độ thanh thải tăng theo tuổi thai và khác nhau giữa các đồng phân R- và S-, cao hơn một chút đối với đồng phân trước (0,244 so với 0,167 L / h). Trẻ sơ sinh non tháng đạt được phơi nhiễm mục tiêu sau 48 giờ với các phác đồ dùng thuốc hiện đang sử dụng. Đầu ra từ các mô phỏng cho thấy có thể đạt được phơi nhiễm mục tiêu với một chiến lược dùng thuốc đơn giản hóa trong 4 ngày đầu điều trị. KẾT LUẬN Độ thanh thải methadone ở trẻ sơ sinh non tháng tăng theo tuổi thai và sự phân bố của nó bị ảnh hưởng bởi tính trắc quang của nó. Các mô phỏng có tính đến những thay đổi về PK phát triển chỉ ra rằng một chiến lược dùng methadone ngắn hơn có thể duy trì phơi nhiễm mục tiêu để kiểm soát các triệu chứng cai nghiện."} +{"idx": 1890030, "en": "BACKGROUND The purpose of this systematic review was to evaluate the association between sleep bruxism (SB) and anxiety symptoms in adults. METHODS A systematic review was performed and studies assessing SB by means of questionnaires, clinical examination and/or polysomnography (PSG), and validated questionnaires to assess anxiety, were included. Search strategies were developed for seven main electronic databases. Risk of bias was assessed using the Joanna Briggs Institute Critical Appraisal Checklist for Analytical Cross-Sectional Studies, and confidence in cumulative evidence was evaluated using the Grading of Recommendations Assessment, Development and Evaluation criteria. RESULTS Eight cross-sectional studies were included, of which five were judged with low and three with moderate risk of bias. No association with SB was observed in three studies that investigated generic levels of anxiety, while other two papers that evaluated generic anxiety levels through the State-Trait Anxiety Inventory (STAI) found a positive association with probable and definite SB in both STAI-1 and STAI-2 subscales. Only one study evaluated dental anxiety in particular and an association with probable SB was observed regarding very anxious or extremely anxious scores. Two studies assessed specific symptoms of anxiety using the panic-agoraphobic spectra evaluation (PAS-SR) questionnaire. Significantly higher PAS-SR total scores were observed in both studies with regard to SB. No study with definitive assessment of SB was identified. CONCLUSION Current literature is controversial regarding an association between SB and generic symptoms of anxiety in adults. It seems that some specific symptoms of the anxiety disorders spectrum might be associated with probable SB.", "gemini": "BỐI CẢNH Mục đích của bài tổng quan hệ thống này là đánh giá mối liên quan giữa nghiến răng khi ngủ (SB) và các triệu chứng lo âu ở người lớn. PHƯƠNG PHÁP Đã thực hiện một bài tổng quan hệ thống và các nghiên cứu đánh giá SB bằng cách sử dụng bảng câu hỏi, khám lâm sàng và/hoặc điện não đồ ngủ (PSG) và các bảng câu hỏi được xác nhận để đánh giá lo âu đã được đưa vào. Các chiến lược tìm kiếm đã được phát triển cho bảy cơ sở dữ liệu điện tử chính. Rủi ro thiên lệch đã được đánh giá bằng cách sử dụng danh sách kiểm tra đánh giá phê bình của Viện Joanna Briggs dành cho các nghiên cứu cắt ngang phân tích và sự tự tin vào bằng chứng tích lũy đã được đánh giá bằng cách sử dụng tiêu chuẩn đánh giá, phát triển và đánh giá khuyến nghị. KẾT QUẢ Có tám nghiên cứu cắt ngang đã được đưa vào, trong đó năm nghiên cứu được đánh giá có rủi ro thiên lệch thấp và ba nghiên cứu có rủi ro thiên lệch trung bình. Không có mối liên quan nào với SB được quan sát thấy trong ba nghiên cứu điều tra các mức độ lo âu chung, trong khi hai bài báo khác đánh giá các mức độ lo âu chung thông qua Bản kiểm kê lo âu trạng thái-nét (STAI) thì thấy có mối liên quan tích cực với SB có thể xảy ra và chắc chắn ở cả hai thang phụ STAI-1 và STAI-2. Chỉ có một nghiên cứu đánh giá cụ thể về lo âu về răng và mối liên quan với SB có thể xảy ra đã được quan sát thấy liên quan đến điểm số rất lo lắng hoặc cực kỳ lo lắng. Hai nghiên cứu đã đánh giá các triệu chứng cụ thể của lo âu bằng cách sử dụng bảng câu hỏi đánh giá phổ hoảng sợ-tránh quảng trường (PAS-SR). Điểm tổng PAS-SR cao hơn đáng kể đã được quan sát thấy trong cả hai nghiên cứu liên quan đến SB. Không có nghiên cứu nào với đánh giá dứt khoát về SB được xác định. KẾT LUẬN Các tài liệu hiện tại còn nhiều tranh cãi về mối liên quan giữa SB và các triệu chứng lo âu chung ở người lớn. Có vẻ như một số triệu chứng cụ thể của phổ rối loạn lo âu có thể liên quan đến SB có thể xảy ra."} +{"idx": 1890031, "en": "BACKGROUND The occurrence of osteoarthritis (OA) of the temporomandibular joint (TMJ) in juveniles could be associated with fluctuating levels of oestrogen and growth hormone (GH) during adolescence. OBJECTIVE To investigate extent of skeletal maturation and predicted adult height in adolescents with TMJ OA. METHOD In total, 155 adolescents (54 males, 101 females; mean age, 14.1 ± 2.0 years) were enrolled. Among them, 19 adolescents (CON) showed no sign of TMD, 50 adolescents exhibited signs of disc displacement but did not have TMJ OA (TMDnoOA), 23 adolescents exhibited the initial stage of TMJ OA (TMJOAini) and 63 adolescents exhibited the severe stage of TMJ OA (TMJOApro). TMJ OA was diagnosed based on the Research Diagnostic Criteria axis I and bone age was estimated using the Greulich-Pyle method. The height, weight and body mass index of participated adolescents were measured and statistically converted to z-scores. The predicted adult height and age of peak height velocity (APHV) were calculated using the BoneXpert® software. RESULTS In female adolescents, the differences between the chronological and bone ages were significantly higher and the z-score for height was significantly lower in the TMJOApro. Female adolescents with TMJOA exhibited a significantly shorter predicted adult height and earlier APHV than those in the CON and TMDnoOA. CONCLUSION The adolescents with TMJ OA exhibited premature skeletal maturations and short predicted adult stature, particularly the female adolescents.", "gemini": "BỐI CẢNH Việc xảy ra bệnh thoái hóa khớp (OA) ở khớp thái dương hàm (TMJ) ở thanh thiếu niên có thể liên quan đến sự dao động nồng độ estrogen và hormone tăng trưởng (GH) trong thời kỳ dậy thì. MỤC TIÊU Để điều tra mức độ trưởng thành của bộ xương và chiều cao dự đoán của người trưởng thành ở thanh thiếu niên mắc TMJ OA. PHƯƠNG PHÁP Tổng cộng có 155 thanh thiếu niên (54 nam, 101 nữ; độ tuổi trung bình, 14,1 ± 2,0 tuổi) đã được tuyển dụng. Trong số đó, 19 thanh thiếu niên (CON) không biểu hiện dấu hiệu nào của RMD, 50 thanh thiếu niên biểu hiện các dấu hiệu lệch đĩa nhưng không bị TMJ OA (TMDnoOA), 23 thanh thiếu niên biểu hiện giai đoạn đầu của TMJ OA (TMJOAini) và 63 thanh thiếu niên biểu hiện giai đoạn nặng của TMJ OA (TMJOApro). TMJ OA được chẩn đoán dựa trên trục I Tiêu chuẩn chẩn đoán nghiên cứu và tuổi xương được ước tính bằng phương pháp Greulich-Pyle. Chiều cao, cân nặng và chỉ số khối cơ thể của thanh thiếu niên tham gia đã được đo và chuyển đổi thống kê thành điểm z. Chiều cao dự đoán của người lớn và tuổi của vận tốc chiều cao đỉnh (APHV) được tính bằng phần mềm BoneXpert®. KẾT QUẢ Ở thanh thiếu niên nữ, sự khác biệt giữa tuổi theo lịch và tuổi xương cao hơn đáng kể và điểm z cho chiều cao thấp hơn đáng kể ở TMJOApro. Thanh thiếu niên nữ mắc TMJOA có chiều cao dự đoán thấp hơn đáng kể và APHV sớm hơn so với nhóm CON và TMDnoOA. KẾT LUẬN Thanh thiếu niên mắc TMJ OA biểu hiện sự trưởng thành của bộ xương sớm và vóc dáng thấp dự đoán của người lớn, đặc biệt là thanh thiếu niên nữ."} +{"idx": 1890032, "en": "Potentially curative treatments for early-stage hepatocellular carcinoma (HCC) have drawbacks and contraindications. Recently, radiotherapy has achieved good outcomes. We compared the outcomes of radiotherapy and radiofrequency ablation (RFA) for early-stage HCC. Consecutive patients with ≤3 early-stage HCC lesions and tumor diameters ≤3 cm treated with RFA or radiotherapy were reviewed. RFA was the first choice for HCC unsuitable for surgery. Otherwise, stereotactic body radiotherapy in five fractions was mainly performed. For HCC adjacent to the gastrointestinal tract, radiotherapy with mild hypofractionation was performed. Propensity score matching was performed to reduce the selection bias between the RFA and radiotherapy groups. Between 2012 and 2016, a total of 231 patients with 474 tumors and 143 patients with 221 tumors were eligible and were treated with RFA and radiotherapy, respectively. In an unmatched comparison, the 3-year local recurrence rate was significantly lower for radiotherapy than for RFA (5.3%; 95% confidence interval [CI], 2.7-9.2; versus 12.9%, 95% CI, 9.9-16.2) (P < 0.01). A propensity score matching analysis of 106 patients in each group successfully matched the two treatment groups with regard to Barcelona Clinic Liver Cancer staging, T stage, and tumor size but not the adjacency of the tumor to risk organs or first or salvage treatment. The 3-year overall survival rates for RFA and radiotherapy patients were comparable (69.1%; 95% CI, 58.2-77.7; and 70.4%; 95% CI, 58.5-79.4, respectively; P = 0.86). Conclusion: Radiotherapy has excellent local control and comparable overall survival in patients with well-compensated liver function, exhibiting advantageous characteristics and compensating for the deficiencies of other treatment modalities; radiotherapy appears to be an acceptable alternative treatment option for patients who are not candidates for RFA.", "gemini": "Các phương pháp điều trị có khả năng chữa khỏi ung thư biểu mô tế bào gan (HCC) giai đoạn đầu có nhược điểm và chống chỉ định. Gần đây, xạ trị đã đạt được kết quả tốt. Chúng tôi đã so sánh kết quả của xạ trị và đốt tần số vô tuyến (RFA) đối với HCC giai đoạn đầu. Các bệnh nhân liên tiếp có ≤3 tổn thương HCC giai đoạn đầu và đường kính khối u ≤3 cm được điều trị bằng RFA hoặc xạ trị đã được xem xét lại. RFA là lựa chọn đầu tiên cho HCC không phù hợp để phẫu thuật. Mặt khác, xạ trị lập thể cơ thể trong năm phân đoạn chủ yếu được thực hiện. Đối với HCC gần đường tiêu hóa, đã thực hiện xạ trị với hạ phân đoạn nhẹ. Ghép điểm khuynh hướng đã được thực hiện để giảm sai lệch lựa chọn giữa các nhóm RFA và xạ trị. Trong khoảng thời gian từ năm 2012 đến năm 2016, tổng cộng có 231 bệnh nhân với 474 khối u và 143 bệnh nhân với 221 khối u đủ điều kiện và được điều trị bằng RFA và xạ trị. Trong một so sánh không khớp, tỷ lệ tái phát tại chỗ sau 3 năm thấp hơn đáng kể đối với xạ trị so với RFA (5,3%; khoảng tin cậy [CI] 95%, 2,7-9,2; so với 12,9%, CI 95%, 9,9-16,2) (P < 0,01). Phân tích ghép điểm khuynh hướng của 106 bệnh nhân trong mỗi nhóm đã ghép thành công hai nhóm điều trị về giai đoạn ung thư gan của Barcelona Clinic, giai đoạn T và kích thước khối u nhưng không phải là sự kề cận của khối u với các cơ quan nguy cơ hoặc điều trị đầu tiên hoặc cứu hộ. Tỷ lệ sống sót chung sau 3 năm đối với bệnh nhân RFA và xạ trị là tương đương (tương ứng là 69,1%; CI 95%, 58,2-77,7; và 70,4%; CI 95%, 58,5-79,4; P = 0,86). Kết luận: Xạ trị có kiểm soát tại chỗ tuyệt vời và tỷ lệ sống sót chung tương đương ở những bệnh nhân có chức năng gan được bù trừ tốt, biểu hiện các đặc điểm có lợi và bù đắp cho những thiếu sót của các phương thức điều trị khác; xạ trị dường như là một lựa chọn điều trị thay thế chấp nhận được cho những bệnh nhân không đủ điều kiện để RFA."} +{"idx": 1890033, "en": "The prominent stromal compartment surrounds pancreatic ductal adenocarcinoma and protects the tumor cells from chemo- or radiotherapy. We hypothesized that our nano formulation carrying cyclopamine (CPA, stroma modulator) and paclitaxel (PTX, antitumor agent) could increase the permeation of PTX through the stromal compartment and improve the intratumoral delivery of PTX. In the present study a sensitive, reliable UPLC-MS/MS method was developed and validated to quantify PTX and CPA simultaneously in mouse whole blood, pancreas, liver and spleen samples. Docetaxel was used as the internal standard. The method demonstrated a linear range of 0.5-2000 ng/mL for whole blood and tissue homogenates for both PTX and CPA. The accuracy and precision of the assay were all within ±15%. Matrix effects for both analytes were within 15%. Recoveries from whole blood, liver, spleen and pancreas homogenates were 92.7-105.2% for PTX and 72.8-99.7% for CPA. The stability was within ±15% in all test biomatrices. The validated method met the acceptance criteria according to US Food and Drug Administration regulatory guidelines. The method was successfully applied to support a pharmacokinetic and biodistribution study for PTX and CPA in mice biomatrices.", "gemini": "Khoang chất nền nổi bật bao quanh ung thư biểu mô ống tụy và bảo vệ các tế bào khối u khỏi hóa trị hoặc xạ trị. Chúng tôi đưa ra giả thuyết rằng công thức nano của chúng tôi mang cyclopamine (CPA, chất điều biến chất nền) và paclitaxel (PTX, tác nhân chống khối u) có thể làm tăng sự thấm của PTX qua khoang chất nền và cải thiện việc đưa PTX vào khối u. Trong nghiên cứu hiện tại, một phương pháp UPLC-MS/MS nhạy, đáng tin cậy đã được phát triển và xác nhận để định lượng PTX và CPA đồng thời trong toàn bộ máu chuột, tụy, gan và mẫu lá lách. Docetaxel được sử dụng làm tiêu chuẩn nội. Phương pháp này chứng minh phạm vi tuyến tính là 0,5-2000 ng/mL đối với toàn bộ máu và dịch mô đồng nhất đối với cả PTX và CPA. Độ chính xác và độ chính xác của xét nghiệm đều trong phạm vi ±15%. Các tác động của ma trận đối với cả hai chất phân tích đều trong phạm vi 15%. Tỷ lệ thu hồi từ toàn bộ máu, gan, lá lách và dịch tụy đồng nhất là 92,7-105,2% đối với PTX và 72,8-99,7% đối với CPA. Độ ổn định nằm trong phạm vi ±15% trong tất cả các sinh học thử nghiệm. Phương pháp được xác nhận đáp ứng các tiêu chí chấp nhận theo hướng dẫn của cơ quan quản lý Thực phẩm và Dược phẩm Hoa Kỳ. Phương pháp này đã được áp dụng thành công để hỗ trợ nghiên cứu dược động học và phân bố sinh học đối với PTX và CPA trong các sinh học chuột."} +{"idx": 1890034, "en": "Pyridoxal 5'-phosphate (PLP)-dependent β-transaminases (βTAs) reversibly catalyze transamination reactions by recognizing amino groups linked to the β-carbon atoms of their substrates. Although several βTA structures have been determined as holo forms containing PLP, little is known about the effect of PLP on the conversion of the apo structure to the holo structure. We determined the crystal structure of the apo form of a βTA from Mesorhizobium sp. strain LUK at 2.2 Å resolution to elucidate how PLP affects the βTA structure. The structure revealed three major disordered regions near the active site. Structural comparison with the holo form also showed that the disordered regions in the apo form are ordered and partially adopt secondary structures in the holo form. These findings suggest that PLP incorporation into the active site contributes to the structural stability of the active site architecture, thereby forming the complete active site. Our results provide novel structural insights into the role of PLP in terms of active site formation.", "gemini": "β-transaminase (βTA) phụ thuộc pyridoxal 5'-phosphate (PLP) xúc tác thuận nghịch các phản ứng chuyển amin bằng cách nhận biết các nhóm amino liên kết với các nguyên tử cacbon β của chất nền của chúng. Mặc dù một số cấu trúc βTA đã được xác định là dạng holo chứa PLP, nhưng người ta vẫn biết rất ít về tác dụng của PLP đối với quá trình chuyển đổi cấu trúc apo thành cấu trúc holo. Chúng tôi đã xác định cấu trúc tinh thể của dạng apo của βTA từ chủng LUK của Mesorhizobium sp. ở độ phân giải 2,2 Å để làm sáng tỏ cách PLP ảnh hưởng đến cấu trúc βTA. Cấu trúc này cho thấy ba vùng mất trật tự chính gần vị trí hoạt động. So sánh cấu trúc với dạng holo cũng cho thấy rằng các vùng mất trật tự trong dạng apo được sắp xếp và một phần áp dụng các cấu trúc bậc hai trong dạng holo. Những phát hiện này cho thấy rằng việc đưa PLP vào vị trí hoạt động góp phần vào sự ổn định về cấu trúc của kiến trúc vị trí hoạt động, do đó tạo thành vị trí hoạt động hoàn chỉnh. Kết quả của chúng tôi cung cấp những hiểu biết mới về mặt cấu trúc về vai trò của PLP trong quá trình hình thành vị trí hoạt động."} +{"idx": 1890035, "en": "OBJECTIVE Patients with chronic stroke have been shown to have failure to release interhemispheric inhibition (IHI) from the intact to the damaged hemisphere before movement execution (premovement IHI). This inhibitory imbalance was found to correlate with poor motor performance in the chronic stage after stroke and has since become a target for therapeutic interventions. The logic of this approach, however, implies that abnormal premovement IHI is causal to poor behavioral outcome and should therefore be present early after stroke when motor impairment is at its worst. To test this idea, in a longitudinal study, we investigated interhemispheric interactions by tracking patients' premovement IHI for one year following stroke. METHODS We assessed premovement IHI and motor behavior five times over a 1-year period after ischemic stroke in 22 patients and 11 healthy participants. RESULTS We found that premovement IHI was normal during the acute/subacute period and only became abnormal at the chronic stage; specifically, release of IHI in movement preparation worsened as motor behavior improved. In addition, premovement IHI did not correlate with behavioral measures cross-sectionally, whereas the longitudinal emergence of abnormal premovement IHI from the acute to the chronic stage was inversely correlated with recovery of finger individuation. INTERPRETATION These results suggest that interhemispheric imbalance is not a cause of poor motor recovery, but instead might be the consequence of underlying recovery processes. These findings call into question the rehabilitation strategy of attempting to rebalance interhemispheric interactions in order to improve motor recovery after stroke. Ann Neurol 2019;85:502-513.", "gemini": "MỤC TIÊU Những bệnh nhân bị đột quỵ mãn tính đã được chứng minh là không giải phóng được sự ức chế liên bán cầu (IHI) từ bán cầu lành sang bán cầu bị tổn thương trước khi thực hiện vận động (IHI trước vận động). Sự mất cân bằng ức chế này được phát hiện có tương quan với hiệu suất vận động kém ở giai đoạn mãn tính sau đột quỵ và kể từ đó đã trở thành mục tiêu của các biện pháp can thiệp điều trị. Tuy nhiên, logic của cách tiếp cận này ngụ ý rằng IHI trước vận động bất thường là nguyên nhân dẫn đến kết quả hành vi kém và do đó nên xuất hiện sớm sau đột quỵ khi tình trạng suy giảm vận động ở mức tồi tệ nhất. Để kiểm tra ý tưởng này, trong một nghiên cứu theo chiều dọc, chúng tôi đã điều tra các tương tác liên bán cầu bằng cách theo dõi IHI trước vận động của bệnh nhân trong một năm sau đột quỵ. PHƯƠNG PHÁP Chúng tôi đã đánh giá IHI trước vận động và hành vi vận động năm lần trong khoảng thời gian một năm sau đột quỵ thiếu máu cục bộ ở 22 bệnh nhân và 11 người tham gia khỏe mạnh. KẾT QUẢ Chúng tôi thấy rằng IHI trước vận động là bình thường trong giai đoạn cấp tính/bán cấp và chỉ trở nên bất thường ở giai đoạn mãn tính; cụ thể là, việc giải phóng IHI khi chuẩn bị vận động trở nên tồi tệ hơn khi hành vi vận động được cải thiện. Ngoài ra, IHI trước vận động không tương quan với các biện pháp hành vi theo chiều ngang, trong khi sự xuất hiện theo chiều dọc của IHI trước vận động bất thường từ giai đoạn cấp tính đến giai đoạn mãn tính lại có tương quan nghịch với sự phục hồi của sự phân biệt ngón tay. DIỄN GIẢI Những kết quả này cho thấy rằng sự mất cân bằng liên bán cầu không phải là nguyên nhân gây ra tình trạng phục hồi vận động kém, mà thay vào đó có thể là hậu quả của các quá trình phục hồi tiềm ẩn. Những phát hiện này đặt ra câu hỏi về chiến lược phục hồi chức năng nhằm cân bằng lại các tương tác liên bán cầu để cải thiện khả năng phục hồi vận động sau đột quỵ. Ann Neurol 2019;85:502-513."} +{"idx": 1890036, "en": "In this work, a series of ten new red-emitting heteroleptic iridium(III) complexes of the type Ir(C^N)2 (L^X) (C^N=cyclometalating ligand, L^X=monoanionic chelating ancillary ligand) is introduced. The suite of new complexes includes two different cyclometalating ligands and five different ancillary ligands, with the primary goal of investigating the effect of the ancillary ligand structure on the excited-state dynamics. The structural variety of the ancillary ligands permitted investigations of the effects of donor atom identity, chelate ring size, and substituents on the electronic structure and excited state properties. Electrochemical analysis showed that the ancillary ligand has a substantial effect on the energy of the HOMO, whereas the LUMO is left unperturbed. Photoluminescence spectra showed that the ancillary ligand can sometimes strongly influence the emission wavelength, but in all cases is an important determinant of the excited-state dynamics.", "gemini": "Trong công trình này, một loạt mười hợp chất iridium(III) dị hợp mới phát ra ánh sáng đỏ loại Ir(C^N)2 (L^X) (C^N=ligand cyclometalating, L^X=ligand phụ chelat hóa đơn anion) được giới thiệu. Bộ hợp chất mới bao gồm hai loại ligand cyclometalating khác nhau và năm loại ligand phụ khác nhau, với mục tiêu chính là nghiên cứu ảnh hưởng của cấu trúc ligand phụ đến động lực trạng thái kích thích. Sự đa dạng về cấu trúc của các phối tử phụ cho phép nghiên cứu các hiệu ứng của bản chất nguyên tử cho, kích thước vòng chelat và các chất thế trên cấu trúc điện tử và các tính chất trạng thái kích thích. Phân tích điện hóa cho thấy rằng phối tử phụ có ảnh hưởng đáng kể đến năng lượng của HOMO, trong khi LUMO không bị nhiễu động. Các phổ phát quang cho thấy rằng đôi khi phối tử phụ có thể ảnh hưởng mạnh đến bước sóng phát xạ, nhưng trong mọi trường hợp, nó là một yếu tố quyết định quan trọng của động lực trạng thái kích thích."} +{"idx": 1890037, "en": "A series of two-dimensionally expanded azulene-core-based π systems have been synthesized with different alkyl chain lengths in the alkoxy moieties connected to the partially oxygen-bridged triarylamine skeletons. The thermal, photophysical, and electronic properties of each compound were evaluated to determine the influence of the alkyl chain length on their effectiveness as hole-transporting materials (HTMs) in perovskite solar cells (PSCs). All the synthesized molecules showed promising material properties, including high solubility, the formation of flat and amorphous films, and optimal alignment of energy levels with perovskites. In particular, the derivatives with methyl and n-butyl in the side chains retained amorphous stability up to 233 and 159 °C, respectively. Such short alkoxy chains also resulted in improved electrical device properties. The PSC device fabricated with the HTM with n-butyl side chains showed the best performance with a power conversion efficiency of 18.9 %, which compares favorably with that of spiro-OMeTAD-based PSCs (spiro-OMeTAD=2,2',7,7'-tetrakis[N,N-bis(p-methoxyphenyl)amino]-9,9'-spirobifluorene).", "gemini": "Một loạt các hệ π dựa trên lõi azulen mở rộng hai chiều đã được tổng hợp với các độ dài chuỗi alkyl khác nhau trong các nhóm alkoxy liên kết với các bộ khung triarylamine có cầu oxy một phần. Các tính chất nhiệt, quang vật lý và điện tử của từng hợp chất đã được đánh giá để xác định ảnh hưởng của độ dài chuỗi alkyl đến hiệu quả của chúng như là vật liệu vận chuyển lỗ (HTM) trong các tế bào năng lượng mặt trời perovskite (PSC). Tất cả các phân tử được tổng hợp đều cho thấy các tính chất vật liệu đầy hứa hẹn, bao gồm độ hòa tan cao, sự hình thành các màng phẳng và vô định hình và sự liên kết tối ưu các mức năng lượng với perovskite. Đặc biệt, các dẫn xuất có metyl và n-butyl trong các chuỗi bên vẫn giữ được độ ổn định vô định hình lên đến 233 và 159 °C. Các chuỗi alkoxy ngắn như vậy cũng dẫn đến các đặc tính thiết bị điện được cải thiện. Thiết bị PSC được chế tạo bằng HTM với các chuỗi bên n-butyl cho thấy hiệu suất tốt nhất với hiệu suất chuyển đổi công suất là 18,9%, có thể so sánh thuận lợi với các PSC dựa trên spiro-OMeTAD (spiro-OMeTAD=2,2',7,7'-tetrakis [N, N-bis(p-methoxyphenyl)amino]-9,9'-spirobifluorene)."} +{"idx": 1890038, "en": "Foam cell formation is a hallmark event during atherosclerosis. The current paradigm is that lipid uptake by a scavenger receptor in macrophages initiates necrosis core formation that characterizes atherosclerosis. We report that NOS1-derived nitric oxide (NO) facilitates low-density lipoprotein (LDL) uptake by macrophages independent of the inflammatory response. LDL uptake could be dramatically suppressed by NOS1 specific inhibitor 1-(2-trifluoromethylphenyl) imidazole (TRIM). Importantly, the notion that NOS1 can mediate uptake of lipoproteins suggests that the foam cell formation is regulated by NOS1-derived NO-mediated mechanism. This is a novel study involving NOS1 as a critical player of foam cell formation and reveals much about the key molecular proteins involved in atherosclerosis. Targeting NOS1 would be a useful strategy in reducing LDL uptake by macrophages and hence dampening the atherosclerosis progression.", "gemini": "Sự hình thành tế bào bọt là một sự kiện đặc trưng trong quá trình xơ vữa động mạch. Mô hình hiện tại là quá trình hấp thụ lipid bởi thụ thể quét rác ở đại thực bào khởi phát sự hình thành nhân hoại tử đặc trưng cho xơ vữa động mạch. Chúng tôi báo cáo rằng oxit nitric (NO) có nguồn gốc từ NOS1 tạo điều kiện cho đại thực bào hấp thụ lipoprotein tỷ trọng thấp (LDL) độc lập với phản ứng viêm. Sự hấp thụ LDL có thể bị ức chế mạnh bởi chất ức chế đặc hiệu NOS1 là 1-(2-trifluoromethylphenyl) imidazole (TRIM). Điều quan trọng là quan niệm rằng NOS1 có thể trung gian sự hấp thụ lipoprotein cho thấy rằng sự hình thành tế bào bọt được điều chỉnh bởi cơ chế trung gian NO có nguồn gốc từ NOS1. Đây là một nghiên cứu mới liên quan đến NOS1 như một tác nhân quan trọng của sự hình thành tế bào bọt và tiết lộ nhiều thông tin về các protein phân tử chính liên quan đến xơ vữa động mạch. Nhắm mục tiêu NOS1 sẽ là một chiến lược hữu ích trong việc giảm sự hấp thụ LDL của đại thực bào và do đó làm giảm sự tiến triển của xơ vữa động mạch."} +{"idx": 1890039, "en": "BACKGROUND Restrictive red blood cell (RBC) transfusion reduces mortality and rebleeding after upper gastrointestinal bleeding (UGIB). However, there is no evidence to guide transfusion strategies in lower gastrointestinal bleeding (LGIB). AIM To assess the association between RBC transfusion strategies and outcomes in patients with LGIB METHODS: This was a post hoc analysis of the UK National Comparative Audit of LGIB and the Use of Blood. The relationships between liberal RBC transfusion and clinical outcomes of rebleeding, mortality and a composite outcome for safe discharge were examined. Transfusion strategy was dichotomised and defined as \"liberal\" when transfusion was administered for haemoglobin (Hb) ≥80 g/L (or ≥90 g/L in patients with acute coronary syndrome) or major haemorrhage, and \"restrictive\" otherwise. Multivariable logistic regression models were used to assess the independent association between liberal RBC transfusion and outcomes. RESULTS Of 2528 consecutive patients enrolled from 143 hospitals in the original study, 666 (26.3%) received RBC transfusion (mean age 73.3 ± 16 years, 49% female, initial mean haemoglobin 90 ± 24 g/L, 2.3% had haemodynamic instability). The rebleeding rate in transfused patients was 42.3%. After adjusting for potential confounders, there was no difference between liberal and restrictive RBC transfusion strategies for the odds of rebleeding (OR 0.89, 95% CI 0.6-1.22), in-hospital mortality (OR 0.54, 95% CI 0.3-1.1) or of achieving the composite outcome (OR 0.72, 95% CI 0.5-1.1). CONCLUSION Although these results could be due to residual confounding, they provide an important foundation for the design of randomised trials to evaluate transfusion strategies for LGIB.", "gemini": "BỐI CẢNH Truyền máu hạn chế hồng cầu (RBC) làm giảm tỷ lệ tử vong và chảy máu lại sau khi xuất huyết tiêu hóa trên (UGIB). Tuy nhiên, không có bằng chứng nào hướng dẫn chiến lược truyền máu trong xuất huyết tiêu hóa dưới (LGIB). MỤC ĐÍCH Đánh giá mối liên quan giữa các chiến lược truyền RBC và kết cục ở những bệnh nhân bị LGIB PHƯƠNG PHÁP: Đây là phân tích hậu cứu của Kiểm toán so sánh quốc gia Vương quốc Anh về LGIB và việc sử dụng máu. Mối quan hệ giữa truyền RBC tự do và kết cục lâm sàng của chảy máu lại, tử vong và kết cục tổng hợp để xuất viện an toàn đã được kiểm tra. Chiến lược truyền máu được phân đôi và định nghĩa là \"tự do\" khi truyền máu được thực hiện đối với hemoglobin (Hb) ≥80 g/L (hoặc ≥90 g/L ở những bệnh nhân có hội chứng mạch vành cấp) hoặc xuất huyết nặng và \"hạn chế\" trong những trường hợp khác. Các mô hình hồi quy logistic đa biến được sử dụng để đánh giá mối liên quan độc lập giữa truyền RBC tự do và kết cục. KẾT QUẢ Trong số 2528 bệnh nhân liên tiếp được tuyển dụng từ 143 bệnh viện trong nghiên cứu ban đầu, 666 (26,3%) được truyền RBC (tuổi trung bình 73,3 ± 16 tuổi, 49% là nữ, hemoglobin trung bình ban đầu 90 ± 24 g/L, 2,3% bị mất ổn định huyết động). Tỷ lệ chảy máu lại ở những bệnh nhân được truyền máu là 42,3%. Sau khi điều chỉnh các yếu tố gây nhiễu tiềm ẩn, không có sự khác biệt giữa các chiến lược truyền RBC tự do và hạn chế đối với tỷ lệ chảy máu lại (OR 0,89, 95% CI 0,6-1,22), tỷ lệ tử vong trong bệnh viện (OR 0,54, 95% CI 0,3-1,1) hoặc đạt được kết cục tổng hợp (OR 0,72, 95% CI 0,5-1,1). KẾT LUẬN Mặc dù những kết quả này có thể là do nhiễu còn sót lại, nhưng chúng cung cấp một nền tảng quan trọng cho thiết kế các thử nghiệm ngẫu nhiên để đánh giá các chiến lược truyền máu cho LGIB."} +{"idx": 1890040, "en": "Hidradenitis suppurativa is a painful chronic inflammatory skin condition characterized by inflammatory nodules that can lead to sinus tracts and scarring. Numerous treatments have been reported, though none have reliable efficacy. Antiinflammatory agents, such as tumor necrosis factor-alpha inhibitors and interleukin inhibitors, have been used as medical therapy for refractory cases. We describe here a case of severe hidradenitis suppurativa in a pediatric patient successfully treated with a combination of high-dose ustekinumab and hyperbaric oxygen therapy.", "gemini": "Viêm tuyến mồ hôi mủ là một tình trạng da viêm mãn tính đau đớn đặc trưng bởi các nốt viêm có thể dẫn đến đường rò và sẹo. Nhiều phương pháp điều trị đã được báo cáo, mặc dù không phương pháp nào có hiệu quả đáng tin cậy. Các tác nhân chống viêm, chẳng hạn như chất ức chế yếu tố hoại tử khối u alpha và chất ức chế interleukin, đã được sử dụng làm liệu pháp điều trị cho các trường hợp khó chữa. Ở đây chúng tôi mô tả một trường hợp viêm tuyến mồ hôi mủ nặng ở một bệnh nhi được điều trị thành công bằng sự kết hợp giữa ustekinumab liều cao và liệu pháp oxy tăng áp."} +{"idx": 1890041, "en": "BACKGROUND Sleep bruxism (SB) is a masticatory muscle activity that affects children. Parents' knowledge is important for its identification and report to dentists. AIM To investigate parents' knowledge about SB among their children. DESIGN A cross-sectional study included 1325 parents of children from dental clinics of seven institutions from all regions of Brazil. Parents answered questions about child's sleep, knowledge about SB and its occurrence among children and parents. SB definition given by parents was dichotomized as \"correct\"/\"incorrect\", based on the American Academy of Sleep Medicine definition. Descriptive, bivariate and multivariate analyses were performed (P < 0.05). RESULTS Most parents (57.3%) did not know what SB is and 88.9% would like to receive more information. SB prevalence among parents was 15.4% and 24.0% among children. Between parents who correctly defined SB, its prevalence increased to 27.5% among parents and 40.6% among children. Parents whose children had/have SB, who would like to receive more information about SB and were from the North, Central-West, Southeast, and South regions were more likely to define SB correctly (P > 0.05). CONCLUSION There is a lack of knowledge of parents about SB. SB among children, parents' interest in receiving more information and their location were factors associated to their knowledge.", "gemini": "BỐI CẢNH Nghiến răng khi ngủ (SB) là hoạt động của cơ nhai ảnh hưởng đến trẻ em. Kiến thức của cha mẹ rất quan trọng để nhận dạng và báo cáo với nha sĩ. MỤC ĐÍCH Điều tra kiến thức của cha mẹ về SB ở con họ. THIẾT KẾ Một nghiên cứu cắt ngang bao gồm 1325 cha mẹ của trẻ em từ các phòng khám nha khoa của bảy cơ sở trên khắp các vùng của Brazil. Cha mẹ trả lời các câu hỏi về giấc ngủ của trẻ, kiến thức về SB và tình trạng xảy ra ở trẻ em và cha mẹ. Định nghĩa SB do cha mẹ đưa ra được phân đôi thành \"đúng\"/\"không đúng\", dựa trên định nghĩa của Học viện Y học Giấc ngủ Hoa Kỳ. Các phân tích mô tả, song biến và đa biến đã được thực hiện (P < 0,05). KẾT QUẢ Hầu hết cha mẹ (57,3%) không biết SB là gì và 88,9% muốn nhận thêm thông tin. Tỷ lệ mắc SB ở cha mẹ là 15,4% và 24,0% ở trẻ em. Trong số những cha mẹ định nghĩa đúng SB, tỷ lệ mắc bệnh tăng lên 27,5% ở cha mẹ và 40,6% ở trẻ em. Những cha mẹ có con bị/đã bị SB, những người muốn nhận thêm thông tin về SB và đến từ các vùng Bắc, Trung-Tây, Đông Nam và Nam có nhiều khả năng định nghĩa đúng về SB (P > 0,05). KẾT LUẬN Cha mẹ còn thiếu kiến thức về SB. SB ở trẻ em, sự quan tâm của cha mẹ trong việc nhận thêm thông tin và vị trí của họ là những yếu tố liên quan đến kiến thức của họ."} +{"idx": 1890042, "en": "A porous polymer coating transfer enrichment method is developed for the direct mass spectrometry (MS) analysis of lipids. The enrichment is fast (ca. 1 min) and enables the profiling and quantitation of lipids in small-volume biofluid samples. Coupled with a photochemical Paternò-Büchi reaction, this method enables the fast determination of lipid structure at the C=C location level and point-of-care lipid biomarker analysis.", "gemini": "Một phương pháp chuyển làm giàu lớp phủ polyme xốp được phát triển để phân tích khối phổ trực tiếp (MS) các lipid. Làm giàu diễn ra nhanh chóng (khoảng 1 phút) và cho phép lập hồ sơ và định lượng lipid trong các mẫu chất lỏng sinh học có thể tích nhỏ. Cùng với phản ứng Paternò-Büchi quang hóa, phương pháp này cho phép xác định nhanh cấu trúc lipid ở mức vị trí C=C và phân tích điểm chỉ định sinh học lipid."} +{"idx": 1890043, "en": "Nucleophilicity parameters (N, sN ) of a group of representative diazaphospholenium hydrides were derived by kinetic investigations of their hydride transfer to a series of reference electrophiles with known electrophilicity (E) values, using the Mayr equation log k2 =sN (N+E). The N scale covers over ten N units, ranging from the most reactive hydride donor (N=25.5) to the least of the scale (N=13.5). This discloses the highest N value ever quantified in terms of Mayr's nucleophilicity scales reported for neutral transition-metal-free hydride donors and implies an exceptional reactivity of this reagent. Even the least reactive hydride donor of this series is still a better hydride donor than those of many other nucleophiles such as the C-H, B-H, Si-H and transition-metal M-H hydride donors. Structure-reactivity analysis reveals that the outstanding hydricity of 2-H-1,3,2-diazaphospholene benefits from the unsaturated skeleton.", "gemini": "Các thông số ái nhân (N, sN) của một nhóm hydride diazaphospholenium đại diện được thu được bằng các nghiên cứu động học về sự chuyển hydride của chúng sang một loạt các chất ưa điện tử tham chiếu có giá trị ưa điện tử (E) đã biết, sử dụng phương trình Mayr log k2 = sN (N+E). Thang N bao phủ hơn mười đơn vị N, từ chất cho hydride phản ứng nhất (N=25,5) đến chất ít nhất trong thang (N=13,5). Điều này tiết lộ giá trị N cao nhất từng được định lượng theo thang ái nhân của Mayr được báo cáo cho các chất cho hydride trung tính không có kim loại chuyển tiếp và ngụ ý phản ứng đặc biệt của thuốc thử này. Ngay cả chất cho hydride phản ứng ít nhất trong loạt chất này vẫn là chất cho hydride tốt hơn so với nhiều chất ái nhân khác như chất cho hydride C-H, B-H, Si-H và kim loại chuyển tiếp M-H. Phân tích cấu trúc-phản ứng cho thấy tính ưa hydride vượt trội của 2-H-1,3,2-diazaphospholene được hưởng lợi từ bộ khung không bão hòa."} +{"idx": 1890044, "en": "OBJECTIVE Avoidant/restrictive food intake disorder (ARFID) is a new diagnosis in the DSM-5 Feeding and Eating Disorders section, for which very limited treatment research has been carried out, yet. A new, 4-week exposure based cognitive behavioral therapy (CBT) day treatment, which integrated the inhibitory learning principles, was developed for adolescents with ARFID, and tested in the current study. METHOD A nonconcurrent multiple baseline design was used in a clinical case series of eleven 10- to 18-year-old patients. After baseline, the 4-week CBT followed. Measurements of DSM-5 ARFID diagnosis, food neophobia and related measures such as body weight and length, were taken at baseline (t1), at the end of the 4-week intensive day treatment (t2) and 3 months after treatment (follow-up, t3). A food selectivity test, a 1-week food diary, and behavioral measures on food intake were also taken at baseline and at 3-month follow-up. Furthermore, continuous measurements of believability of dysfunctional cognitions, anxiety, and food acceptance were taken throughout the 4-weeks day treatment. RESULTS At follow-up, 10 out of 11 patients were in remission and had a healthy body weight and an average, age-adequate nutritional intake. For most patients, food neophobia scores decreased to a nonclinical range. The belief in dysfunctional cognitions and anxiety levels decreased during treatment. DISCUSSION This new exposure-based CBT for adolescents with ARFID seems promising. These results may be very useful for clinical practice and stimulate further development of effective CBT interventions in the area of ARFID.", "gemini": "MỤC TIÊU Rối loạn ăn uống hạn chế/tránh né (ARFID) là một chẩn đoán mới trong phần Rối loạn ăn uống và cho ăn của DSM-5, trong đó nghiên cứu điều trị rất hạn chế đã được thực hiện. Một liệu pháp điều trị nhận thức hành vi (CBT) dựa trên phơi nhiễm 4 tuần mới, tích hợp các nguyên tắc học tập ức chế, đã được phát triển cho thanh thiếu niên mắc ARFID và được thử nghiệm trong nghiên cứu hiện tại. PHƯƠNG PHÁP Thiết kế đường cơ sở nhiều không đồng thời đã được sử dụng trong một loạt trường hợp lâm sàng gồm mười một bệnh nhân từ 10 đến 18 tuổi. Sau đường cơ sở, CBT kéo dài 4 tuần đã được thực hiện. Các phép đo chẩn đoán ARFID DSM-5, chứng sợ thức ăn mới và các biện pháp liên quan như cân nặng và chiều dài cơ thể đã được thực hiện tại đường cơ sở (t1), vào cuối đợt điều trị trong ngày chuyên sâu kéo dài 4 tuần (t2) và 3 tháng sau khi điều trị (theo dõi, t3). Một bài kiểm tra chọn lọc thực phẩm, nhật ký thực phẩm trong 1 tuần và các biện pháp hành vi về lượng thức ăn cũng được thực hiện tại đường cơ sở và theo dõi sau 3 tháng. Hơn nữa, các phép đo liên tục về khả năng tin tưởng của các nhận thức rối loạn chức năng, lo lắng và chấp nhận thực phẩm đã được thực hiện trong suốt 4 tuần điều trị trong ngày. KẾT QUẢ Khi theo dõi, 10 trên 11 bệnh nhân đã thuyên giảm và có cân nặng khỏe mạnh và lượng dinh dưỡng trung bình, phù hợp với độ tuổi. Đối với hầu hết các bệnh nhân, điểm sợ thức ăn mới đã giảm xuống mức không lâm sàng. Niềm tin vào các nhận thức rối loạn chức năng và mức độ lo lắng giảm trong quá trình điều trị. THẢO LUẬN CBT dựa trên phơi nhiễm mới này dành cho thanh thiếu niên mắc ARFID có vẻ hứa hẹn. Những kết quả này có thể rất hữu ích cho thực hành lâm sàng và thúc đẩy phát triển thêm các biện pháp can thiệp CBT hiệu quả trong lĩnh vực ARFID."} +{"idx": 1890045, "en": "The correlation of mycobactericidal property of macrophages with its potential to deliver bacteria to hydrolytic lysosomes, augmented with ubiquitin-derived peptides (Ub2), activates the process of autophagy. This leads to the formation of phagolysosomes supported by factor involving increased cationic charges which regulate the acidic pH causing elimination of Mycobacterium. To better understand this interaction of cationic-rich ubiquitin-derived peptides with mycobacteria and to identify putative mycobacterial intrinsic resistance mechanisms for phagolysosome formation, we have synthesized a new series of Ub2 peptides, wherein the Gly residues are replaced with azaGly with the aim to improve metabolic stability. In addition to that a new methodology is reported for the synthesis of heteroaryl tethered peptides using azaGly as a linker. We have demonstrated that positive puncta (directly proportional to the acidification of lysosome) in cytosol was significantly increased after 6 hours on the treatment of macrophage with Ub2 peptide derivatives (1, 6, 10, and 11) causing the higher intensity of lysosome observed through LysoTracker Red Dye. The circular dichroism spectral studies are carried out in water and water:TFE mixture and demonstrated that the Ub2 peptides have helix-forming tendency in the presence of TFE. The recognizable intracellular killing of Mycobacterium tuberculosis by Ub2 peptides provides a new approach for host-directed therapy.", "gemini": "Sự tương quan của đặc tính diệt khuẩn của đại thực bào với tiềm năng đưa vi khuẩn vào lysosome thủy phân, tăng cường với các peptide có nguồn gốc từ ubiquitin (Ub2), kích hoạt quá trình autophagy. Điều này dẫn đến sự hình thành các phagolysosome được hỗ trợ bởi yếu tố liên quan đến việc tăng điện tích dương điều chỉnh độ pH axit gây ra sự loại bỏ Mycobacterium. Để hiểu rõ hơn về sự tương tác này của các peptide có nguồn gốc từ ubiquitin giàu cation với vi khuẩn mycobacteria và xác định các cơ chế kháng thuốc nội tại của vi khuẩn mycobacteria đối với sự hình thành phagolysosome, chúng tôi đã tổng hợp một loạt peptide Ub2 mới, trong đó các dư lượng Gly được thay thế bằng azaGly với mục đích cải thiện sự ổn định về mặt chuyển hóa. Ngoài ra, một phương pháp mới được báo cáo để tổng hợp các peptide liên kết heteroaryl sử dụng azaGly làm chất liên kết. Chúng tôi đã chứng minh rằng các điểm chấm dương (tỷ lệ thuận với độ axit hóa của lysosome) trong tế bào chất tăng đáng kể sau 6 giờ xử lý đại thực bào bằng các dẫn xuất peptide Ub2 (1, 6, 10 và 11) gây ra cường độ cao hơn của lysosome quan sát được thông qua thuốc nhuộm đỏ LysoTracker. Các nghiên cứu phổ dị sắc tròn được thực hiện trong hỗn hợp nước và nước: TFE và chứng minh rằng các peptide Ub2 có xu hướng hình thành xoắn ốc khi có TFE. Việc tiêu diệt Mycobacterium tuberculosis trong tế bào có thể nhận biết được bằng các peptide Ub2 cung cấp một cách tiếp cận mới cho liệu pháp hướng đến vật chủ."} +{"idx": 1890046, "en": "We used RNA-sequencing (RNA-Seq) technology and an old hematopoietic stem and progenitor cells (HSPCs) model in vitro to analyze differential expressions of mismatch repair (MMR)-related genes in aged HSPCs, so as to explore the mechanism of DNA MMR injury in hematopoietic stem cells (HSC) aging. In this study, by combining RNA-seq data and National Center for Biotechnology Information database, we focus on six widely reported MMR genes MSH2, MSH3, MSH6, MLH1, PMS1, PMS2, and five MMR genes with closer ties to HSC aging Pcna, Exo1, Rpa1, Rpa2, and Rpa3 according to the genes functional classification and the related signaling pathway. It is concluded that MMR is closely related to HSC aging. This study provides experimental evidence for future researching MMR in HSC aging.", "gemini": "Chúng tôi đã sử dụng công nghệ giải trình tự RNA (RNA-Seq) và một mô hình tế bào gốc tạo máu và tiền thân (HSPC) trong ống nghiệm cũ để phân tích các biểu hiện khác biệt của các gen liên quan đến sửa chữa không khớp (MMR) trong các HSPC đã lão hóa, để khám phá cơ chế tổn thương MMR DNA trong tế bào gốc tạo máu (HSC) lão hóa. Trong nghiên cứu này, bằng cách kết hợp dữ liệu RNA-seq và cơ sở dữ liệu của Trung tâm Thông tin Công nghệ Sinh học Quốc gia, chúng tôi tập trung vào sáu gen MMR được báo cáo rộng rãi là MSH2, MSH3, MSH6, MLH1, PMS1, PMS2 và năm gen MMR có mối liên hệ chặt chẽ hơn với Pcna lão hóa HSC, Exo1, Rpa1, Rpa2 và Rpa3 theo phân loại chức năng gen và con đường truyền tín hiệu liên quan. Kết luận là MMR có liên quan chặt chẽ đến quá trình lão hóa HSC. Nghiên cứu này cung cấp bằng chứng thực nghiệm cho nghiên cứu MMR trong tương lai về quá trình lão hóa HSC."} +{"idx": 1890047, "en": "Ankylosing spondylitis (AS) is a chronic rheumatic disease which mainly affects the axial skeleton and sacroiliac joints. T-helper 17 (Th17) cells have been reportedly involved in AS pathogenesis. Nanocurcumin is considered to be beneficial, as an anti-inflammatory compound, in AS patients treatment. In this study, Th17-related immunological parameters were evaluated in AS patients. Transcription factors messenger RNA (mRNA) expression level, cytokines, and related microRNAs (miRNAs) were measured by real-time polymerase chain reaction. Additionally, Th17 frequency and cytokines secretion were evaluated by flow cytometry and enzyme-linked immunosorbent assay tests, respectively. The frequency of Th17 was higher in AS patients. Gained data from nanocurcumin group also demonstrated that retinoic acid-related orphan receptor γ (RORγt) and interleukin-17 (IL-17) mRNA expression levels were significantly decreased (P = 0.0001 and 0.0006, respectively), as the decrease also happened in Th17-associated miRNAs including miR-141, miR-155, and miR-200 ( P = 0.04, P = 0.02, and P < 0.0001, respectively). Posttreatment data of miR-155 and miR-200 in the nanocurcumin and placebo groups also showed a higher expression level in the placebo group compared with nanocurcumin-treated patients. Some clinical symptoms of AS patients were also improved at the end of the treatment process. The results of this study showed the potential ability of nanocurcumin to regulate Th17 cells activity in AS patients. This study provided further evidence on the function and underlying mechanism of nanocurcumin helping better treatment of AS.", "gemini": "Viêm cột sống dính khớp (AS) là một bệnh thấp khớp mãn tính chủ yếu ảnh hưởng đến bộ xương trục và các khớp cùng chậu. Các tế bào T helper 17 (Th17) được báo cáo là có liên quan đến sinh bệnh AS. Nanocurcumin được coi là có lợi, như một hợp chất chống viêm, trong điều trị bệnh nhân AS. Trong nghiên cứu này, các thông số miễn dịch liên quan đến Th17 đã được đánh giá ở bệnh nhân AS. Mức biểu hiện RNA thông tin (mRNA) của các yếu tố phiên mã, cytokine và microRNA (miRNA) liên quan được đo bằng phản ứng chuỗi polymerase thời gian thực. Ngoài ra, tần số Th17 và tiết cytokine được đánh giá lần lượt bằng phương pháp đo lưu lượng tế bào và xét nghiệm miễn dịch hấp phụ liên kết enzyme. Tần số Th17 cao hơn ở bệnh nhân AS. Dữ liệu thu được từ nhóm nanocurcumin cũng chứng minh rằng mức biểu hiện mRNA của thụ thể mồ côi liên quan đến axit retinoic γ (RORγt) và interleukin-17 (IL-17) giảm đáng kể (P = 0,0001 và 0,0006, tương ứng), vì sự giảm cũng xảy ra ở các miRNA liên quan đến Th17 bao gồm miR-141, miR-155 và miR-200 (P = 0,04, P = 0,02 và P < 0,0001, tương ứng). Dữ liệu sau điều trị của miR-155 và miR-200 trong nhóm nanocurcumin và nhóm giả dược cũng cho thấy mức biểu hiện cao hơn ở nhóm giả dược so với bệnh nhân được điều trị bằng nanocurcumin. Một số triệu chứng lâm sàng của bệnh nhân AS cũng được cải thiện vào cuối quá trình điều trị. Kết quả nghiên cứu này cho thấy khả năng tiềm tàng của nanocurcumin trong việc điều chỉnh hoạt động của tế bào Th17 ở bệnh nhân AS. Nghiên cứu này cung cấp thêm bằng chứng về chức năng và cơ chế cơ bản của nanocurcumin giúp điều trị AS tốt hơn."} +{"idx": 1890048, "en": "OBJECTIVE The aim of the study is to examine the association between victimization and unhealthy weight control behaviors (UWCB), accounting for other key correlates of UWCB while considering the moderating role of sexual identity and gender. METHOD This study used data from the 2017 Texas Youth Risk Behavior Survey (YRBS), a representative sample of students in grades 9-12 in the U.S. state of Texas, including 2,067 students (1,754 heterosexuals and 313 sexual minorities). We used survey-weighted logistic regression to examine the association of sexual identity, gender, and victimization (dating violence, cyberbullying, and school bullying) with UWCB, after adjusting for demographic information and indicators of weight, physical activity, and support from adults. RESULTS Unhealthy weight control behaviors were associated with older age, being obese, lack of support from adults, low physical activity, and cyberbullying. A significant three-way interaction between gender, sexual identity and bullying showed that bullying was associated with high levels of UWCB among sexual minority males. DISCUSSION Our study highlights the role of victimization in health behaviors for stigmatized groups, and the importance of school efforts to implement an equitable and safe learning environment for all students.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu này là kiểm tra mối liên quan giữa nạn nhân và các hành vi kiểm soát cân nặng không lành mạnh (UWCB), tính đến các mối tương quan chính khác của UWCB trong khi xem xét vai trò điều tiết của bản dạng giới tính và giới tính. PHƯƠNG PHÁP Nghiên cứu này sử dụng dữ liệu từ Khảo sát hành vi nguy cơ của thanh thiếu niên Texas năm 2017 (YRBS), một mẫu đại diện của học sinh lớp 9-12 tại tiểu bang Texas của Hoa Kỳ, bao gồm 2.067 học sinh (1.754 người dị tính và 313 người thiểu số tình dục). Chúng tôi đã sử dụng hồi quy logistic có trọng số khảo sát để kiểm tra mối liên quan giữa bản dạng giới tính, giới tính và nạn nhân (bạo lực khi hẹn hò, bắt nạt trên mạng và bắt nạt ở trường) với UWCB, sau khi điều chỉnh thông tin nhân khẩu học và các chỉ số về cân nặng, hoạt động thể chất và sự hỗ trợ từ người lớn. KẾT QUẢ Các hành vi kiểm soát cân nặng không lành mạnh có liên quan đến tuổi cao hơn, béo phì, thiếu sự hỗ trợ từ người lớn, ít hoạt động thể chất và bắt nạt trên mạng. Một tương tác ba chiều đáng kể giữa giới tính, bản dạng giới tính và bắt nạt cho thấy bắt nạt có liên quan đến mức độ UWCB cao ở nam giới là người thiểu số tình dục. THẢO LUẬN Nghiên cứu của chúng tôi làm nổi bật vai trò của nạn nhân trong hành vi sức khỏe đối với các nhóm bị kỳ thị và tầm quan trọng của các nỗ lực của trường học nhằm thực hiện một môi trường học tập công bằng và an toàn cho tất cả học sinh."} +{"idx": 1890049, "en": "According to the \"parent-offspring conflict hypothesis\" the rapid evolution and diversification of the mammalian placenta is driven by divergent optima of resource allocation between fetus and mother. The fetus has an interest to maximize its resource intake, while the mother has an interest to restrict the transfer of resources, and thus retain resources for subsequent pregnancies. In the epitheliochorial placenta, the contacting fetal and maternal surfaces at the feto-maternal interface are covered with microvilli, which leads to an increase of membrane surfaces available for transport processes. Because membranes are the site of active transport, the conflict hypothesis predicts that the fetal surfaces at the feto-maternal interfaces are larger than the maternal ones. We use transmission electron microscopy and a stereological method to estimate the factors by which the apical fetal and maternal membranes are enlarged by the microvilli. Ten species with an epitheliochorial placenta were studied. Focused ion beam-scanning electron microscopy (FIB-SEM) was used to create three-dimensional models of the interdigitating microvilli of the bovine and porcine placenta. In all species, the fetal surface was larger than the maternal. This was due to a higher number of fetal microvilli and to the presence of membrane folds at the base of the fetal, but not of maternal microvilli. Our results suggest that the ultrastructural morphology of the feto-maternal interface in the epitheliochorial placenta is shaped by conflicting interests between fetus and mother and thus represent a so far neglected arena of the parent-offspring conflict.", "gemini": "Theo \"giả thuyết xung đột cha mẹ - con cái\", sự tiến hóa nhanh chóng và sự đa dạng của nhau thai ở động vật có vú được thúc đẩy bởi các tối ưu phân bổ nguồn lực khác nhau giữa thai nhi và mẹ. Thai nhi có lợi ích tối đa hóa lượng tài nguyên nạp vào, trong khi mẹ có lợi ích hạn chế việc chuyển giao tài nguyên, do đó giữ lại tài nguyên cho những lần mang thai tiếp theo. Ở nhau thai biểu mô, các bề mặt tiếp xúc của thai nhi và mẹ tại giao diện mẹ - con được bao phủ bởi các vi nhung mao, dẫn đến sự gia tăng bề mặt màng có sẵn cho các quá trình vận chuyển. Vì màng là nơi vận chuyển tích cực, nên giả thuyết xung đột dự đoán rằng các bề mặt của thai nhi tại giao diện mẹ - con lớn hơn so với bề mặt của mẹ. Chúng tôi sử dụng kính hiển vi điện tử truyền qua và phương pháp lập thể để ước tính các yếu tố mà các màng vi nhung mao của thai nhi và mẹ được mở rộng. Mười loài có nhau thai biểu mô đã được nghiên cứu. Kính hiển vi điện tử quét chùm ion tập trung (FIB-SEM) được sử dụng để tạo ra các mô hình ba chiều của các vi nhung mao xen kẽ của nhau thai ở bò và lợn. Ở tất cả các loài, bề mặt của thai nhi đều lớn hơn bề mặt của mẹ. Điều này là do số lượng vi nhung mao của thai nhi nhiều hơn và sự hiện diện của các nếp gấp màng ở gốc của vi nhung mao của thai nhi, nhưng không phải của vi nhung mao của mẹ. Kết quả của chúng tôi cho thấy rằng hình thái siêu cấu trúc của giao diện mẹ - con ở nhau thai biểu mô được định hình bởi những lợi ích xung đột giữa thai nhi và mẹ và do đó đại diện cho một lĩnh vực cho đến nay vẫn bị bỏ qua của xung đột cha mẹ - con cái."} +{"idx": 1890050, "en": "We used blood serum samples collected from 31 lung cancer (LC) patients and 29 healthy volunteers in this study. Levels of serum metabolites were qualitative quantified with gas chromatography-mass spectrometry (GC-MS), and the data were analyzed by partial least-squares discrimination analysis (PLS-DA). Based on the Kyoto Encyclopedia of Genes and Genomes database, we performed pathway-based analysis utilizing metabolites presented at differential abundance between the LC serum samples and the normal healthy serum samples for systematical investigation on the metabolic alterations associated with LC pathogenesis. Finally, we analyzed the significantly enriched pathways as well as their relevant differentially expressed messenger RNAs, and drawn a correlation network plot to identify the serum metabolic biomarkers and the significantly altered metabolic pathways for LC. GC-MS analysis showed that 23 of the 169 metabolites identified were significantly different. PLS-DA model revealed that 13 of these metabolites were with variable importance > 1, and particularly five were with area under curve > 0.9. Pathway-based analysis demonstrated that five of eight enriched metabolic pathways were statistically significant with false discovery rate < 0.05. Lastly, the correlation networks between these pathways and their related genes suggested that 29 genes had correlation degree > 10, which were mainly engaged in the purine metabolism. In conclusion, we identified indole-3-lactate, erythritol, adenosine-5-phosphate, paracetamol and threitol as serum metabolic biomarkers for LC through metabolomics analysis. Besides, we identified the purine metabolism as the significantly altered metabolic pathway in LC with the help of transcriptomics analysis.", "gemini": "Chúng tôi đã sử dụng các mẫu huyết thanh máu được thu thập từ 31 bệnh nhân ung thư phổi (LC) và 29 tình nguyện viên khỏe mạnh trong nghiên cứu này. Nồng độ các chất chuyển hóa trong huyết thanh được định lượng định tính bằng phương pháp sắc ký khí - khối phổ (GC-MS) và dữ liệu được phân tích bằng phương pháp phân tích phân biệt theo bình phương tối thiểu từng phần (PLS-DA). Dựa trên cơ sở dữ liệu Bách khoa toàn thư về gen và bộ gen của Kyoto, chúng tôi đã thực hiện phân tích dựa trên con đường bằng cách sử dụng các chất chuyển hóa có sự khác biệt về nồng độ giữa các mẫu huyết thanh LC và các mẫu huyết thanh khỏe mạnh bình thường để điều tra có hệ thống về những thay đổi chuyển hóa liên quan đến bệnh sinh LC. Cuối cùng, chúng tôi đã phân tích các con đường được làm giàu đáng kể cũng như các RNA thông tin biểu hiện khác biệt có liên quan của chúng và vẽ biểu đồ mạng tương quan để xác định các dấu ấn sinh học chuyển hóa trong huyết thanh và các con đường chuyển hóa bị thay đổi đáng kể đối với LC. Phân tích GC-MS cho thấy 23 trong số 169 chất chuyển hóa được xác định có sự khác biệt đáng kể. Mô hình PLS-DA cho thấy 13 trong số các chất chuyển hóa này có độ quan trọng khác nhau> 1 và đặc biệt là năm chất có diện tích dưới đường cong> 0,9. Phân tích dựa trên con đường chứng minh rằng năm trong số tám con đường chuyển hóa được làm giàu có ý nghĩa thống kê với tỷ lệ khám phá sai <0,05. Cuối cùng, các mạng tương quan giữa các con đường này và các gen liên quan của chúng cho thấy 29 gen có độ tương quan> 10, chủ yếu tham gia vào quá trình chuyển hóa purin. Tóm lại, chúng tôi đã xác định indole-3-lactate, erythritol, adenosine-5-phosphate, paracetamol và threitol là các dấu ấn sinh học chuyển hóa trong huyết thanh đối với LC thông qua phân tích chuyển hóa. Bên cạnh đó, chúng tôi xác định quá trình chuyển hóa purin là con đường chuyển hóa bị thay đổi đáng kể trong LC với sự trợ giúp của phân tích phiên mã."} +{"idx": 1890051, "en": "Palladium-catalyzed decarboxylative alkynylation of α-acyloxyketones triggered by C(sp3 )-O bond cleavage is disclosed. The decarboxylation strategy featuring a neutral reaction condition enabled an unprecedent catalytic alkynylation of a ketone enolate. The reaction was applied to a variety of substrates, giving desired products in good yields. We successfully obtained X-ray crystallography of a new palladium-enolate intermediate that was synthesized by a reaction of [Pd(cod)(CH2 TMS)2 ] with XPhos and α-acyloxyketone at room temperature, indicating facile C(sp3 )-O bond disconnection.", "gemini": "Phản ứng alkynyl hóa khử carboxyl xúc tác bởi paladi của α-acyloxyketone được kích hoạt bởi sự cắt liên kết C(sp3 )-O được tiết lộ. Chiến lược khử carboxyl có đặc điểm là điều kiện phản ứng trung tính cho phép alkynyl hóa xúc tác chưa từng có của enolat ketone. Phản ứng được áp dụng cho nhiều loại chất nền khác nhau, tạo ra các sản phẩm mong muốn với hiệu suất tốt. Chúng tôi đã thu được thành công tinh thể học tia X của một chất trung gian enolat-paladi mới được tổng hợp bằng phản ứng của [Pd(cod)(CH2 TMS)2 ] với XPhos và α-acyloxyketone ở nhiệt độ phòng, cho thấy sự ngắt kết nối liên kết C(sp3 )-O dễ dàng."} +{"idx": 1890052, "en": "BACKGROUND Oral health policy and funding must be informed by well-designed studies which monitor oral health and the factors which influence it. AIM This study aimed to analyse the oral health of the Longitudinal Study of Australia Children (LSAC). DESIGN The LSAC is a dual-cohort cross-sequential study run biennially since 2004. Carer-report measures for oral health were measured across six biennial waves and included frequency of tooth brushing, dental service use, and dental problems since the previous wave. RESULTS A total of 10 090 Australian children participated at baseline (birth [B] n = 5017 and kindergarten [K] n = 4983). Most carers reported that children brushed daily and had regular access to dental care. Increasing age was a significant predictor of dental caries, whereas no differences were observed between time periods and cohorts. Dental caries was more frequently reported than dental injuries. Caries was highest at age eight for the B (n = 1234, 30.5%) and K (n = 1355, 31.5%) cohorts. CONCLUSIONS By the age of six, caries prevalence had already begun to climb despite the majority of carers reporting good oral health behaviours for their children. Early intervention in the prevention of dental caries is essential, as children appear to attend dental services when caries is already occurring.", "gemini": "BỐI CẢNH Chính sách và tài trợ về sức khỏe răng miệng phải được thông báo bởi các nghiên cứu được thiết kế tốt để theo dõi sức khỏe răng miệng và các yếu tố ảnh hưởng đến sức khỏe răng miệng. MỤC ĐÍCH Nghiên cứu này nhằm mục đích phân tích sức khỏe răng miệng của Nghiên cứu theo chiều dọc về trẻ em Úc (LSAC). THIẾT KẾ LSAC là một nghiên cứu theo chiều dọc theo dõi kép được tiến hành hai năm một lần kể từ năm 2004. Các biện pháp báo cáo của người chăm sóc về sức khỏe răng miệng đã được đo lường qua sáu đợt hai năm một lần và bao gồm tần suất đánh răng, sử dụng dịch vụ nha khoa và các vấn đề về răng kể từ đợt trước. KẾT QUẢ Tổng cộng có 10.090 trẻ em Úc tham gia tại thời điểm ban đầu (sinh [B] n = 5017 và mẫu giáo [K] n = 4983). Hầu hết những người chăm sóc đều báo cáo rằng trẻ em đánh răng hàng ngày và được tiếp cận thường xuyên với dịch vụ chăm sóc nha khoa. Tuổi càng cao là yếu tố dự báo đáng kể về sâu răng, trong khi không có sự khác biệt nào được quan sát thấy giữa các thời kỳ và nhóm đối tượng. Sâu răng được báo cáo thường xuyên hơn so với chấn thương răng. Sâu răng cao nhất ở độ tuổi tám đối với nhóm B (n = 1234, 30,5%) và nhóm K (n = 1355, 31,5%). KẾT LUẬN Đến sáu tuổi, tỷ lệ sâu răng đã bắt đầu tăng lên mặc dù phần lớn những người chăm sóc báo cáo rằng trẻ em của họ có hành vi sức khỏe răng miệng tốt. Can thiệp sớm để phòng ngừa sâu răng là rất cần thiết, vì trẻ em có vẻ như chỉ đến dịch vụ nha khoa khi sâu răng đã xảy ra."} +{"idx": 1890053, "en": "A tree-like hierarchical branching structure is present in many biological systems, such as the kidney, lung, mammary gland, and blood vessels. Most of these organs form through branching morphogenesis, where outward growth results in smaller and smaller branches. However, the blood vasculature is unique in that it exists as two trees (arterial and venous) connected at their tips. Obtaining this organization might therefore require unique developmental mechanisms. As reviewed here, recent data indicate that arterial trees often form in reverse order. Accordingly, initial arterial endothelial cell differentiation occurs outside of arterial vessels. These pre-artery cells then build trees by following a migratory path from smaller into larger arteries, a process guided by the forces imparted by blood flow. Thus, in comparison to other branched organs, arteries can obtain their structure through inward growth and coalescence. Here, new information on the underlying mechanisms is discussed, and how defects can lead to pathologies, such as hypoplastic arteries and arteriovenous malformations.", "gemini": "Cấu trúc phân nhánh theo thứ bậc giống như cây có mặt trong nhiều hệ thống sinh học, chẳng hạn như thận, phổi, tuyến vú và mạch máu. Hầu hết các cơ quan này hình thành thông qua quá trình hình thái học phân nhánh, trong đó sự phát triển ra bên ngoài dẫn đến các nhánh ngày càng nhỏ hơn. Tuy nhiên, hệ thống mạch máu là duy nhất ở chỗ nó tồn tại dưới dạng hai cây (động mạch và tĩnh mạch) được kết nối ở đầu của chúng. Do đó, để có được tổ chức này có thể đòi hỏi các cơ chế phát triển độc đáo. Theo như đánh giá ở đây, dữ liệu gần đây chỉ ra rằng các cây động mạch thường hình thành theo thứ tự ngược lại. Theo đó, sự biệt hóa tế bào nội mô động mạch ban đầu xảy ra bên ngoài các mạch động mạch. Sau đó, các tế bào tiền động mạch này xây dựng các cây bằng cách đi theo một con đường di chuyển từ động mạch nhỏ hơn vào động mạch lớn hơn, một quá trình được hướng dẫn bởi các lực do dòng máu tạo ra. Do đó, so với các cơ quan phân nhánh khác, động mạch có thể có được cấu trúc của chúng thông qua sự phát triển hướng vào trong và sự hợp nhất. Ở đây, thông tin mới về các cơ chế cơ bản được thảo luận và cách các khiếm khuyết có thể dẫn đến các bệnh lý, chẳng hạn như động mạch kém phát triển và dị dạng động tĩnh mạch."} +{"idx": 1890054, "en": "In the liver tissues of obese diabetic or nondiabetic patients, triggering receptor expressed on myeloid cells-1 (TREM-1) is usually found to be upregulated, thus leading to upregulation of various inflammatory cytokines and lipid accumulation. On the other hand, nonalcoholic fatty liver disease (NAFLD), characterized by excess lipid accumulation, and inflammatory injury in liver, is becoming an epidemic disease, globally. In the present study, we aimed to investigate the biological role and the underlying mechanisms of TREM-1 in NAFLD. upregulation of TREM-1 occurred in high-fat diet (HFD)-induced mice NAFLD model and oleic acid-treated HepG2 and primary mouse hepatocytes cell model at messenger RNA and protein levels. Functional studies established that overexpression of TREM-1 displayed hyperlipidemia, and increased in inflammatory indicators and lipid accumulation-related genes, which was ameliorated by knockdown of TREM-1. Our results also showed that obvious lipid accumulation and inflammatory injury occurred in the liver tissue of HFD-fed mice, while treatment with lentiviral vector short hairpin TREM showed marked improvement in tissue morphology and architecture and less lipid accumulation, thus deciphering the mechanism through which knockdown of TREM-1 ameliorated the inflammatory response and lipid accumulation of NAFLD mice through inactivation of the nuclear factor-κB (NF-κB) and PI3K/AKT signal pathways, respectively. In conclusion, TREM-1/NF-κB and TREM-1/PI3K/AKT axis could be an important mechanism in ameliorating the inflammatory response and lipid accumulation, respectively, thus shedding light on the development of novel therapeutics to the treatment of NAFLD.", "gemini": "Trong các mô gan của bệnh nhân tiểu đường béo phì hoặc không tiểu đường, thụ thể kích hoạt được biểu hiện trên tế bào myeloid-1 (TREM-1) thường được phát hiện là tăng điều hòa, do đó dẫn đến tăng điều hòa các cytokine gây viêm khác nhau và tích tụ lipid. Mặt khác, bệnh gan nhiễm mỡ không do rượu (NAFLD), đặc trưng bởi sự tích tụ lipid dư thừa và tổn thương viêm ở gan, đang trở thành một bệnh dịch toàn cầu. Trong nghiên cứu hiện tại, chúng tôi nhằm mục đích điều tra vai trò sinh học và cơ chế cơ bản của TREM-1 trong NAFLD. Sự tăng điều hòa của TREM-1 xảy ra ở mô hình NAFLD chuột gây ra bởi chế độ ăn nhiều chất béo (HFD) và mô hình tế bào gan chính của chuột và HepG2 được xử lý bằng axit oleic ở mức RNA thông tin và protein. Các nghiên cứu chức năng đã thiết lập rằng sự biểu hiện quá mức của TREM-1 biểu hiện chứng tăng lipid máu và tăng các chỉ số viêm và gen liên quan đến tích tụ lipid, được cải thiện bằng cách hạ gục TREM-1. Kết quả của chúng tôi cũng cho thấy sự tích tụ lipid rõ ràng và tổn thương viêm xảy ra ở mô gan của chuột ăn HFD, trong khi điều trị bằng vectơ tóc kẹp ngắn lentivirus TREM cho thấy sự cải thiện rõ rệt về hình thái và cấu trúc mô và ít tích tụ lipid hơn, do đó giải mã cơ chế hạ gục TREM-1 cải thiện đáp ứng viêm và tích tụ lipid ở chuột NAFLD thông qua quá trình bất hoạt các con đường tín hiệu nhân tố hạt nhân-κB (NF-κB) và PI3K/AKT, tương ứng. Tóm lại, trục TREM-1/NF-κB và TREM-1/PI3K/AKT có thể là một cơ chế quan trọng trong việc cải thiện đáp ứng viêm và tích tụ lipid, do đó làm sáng tỏ sự phát triển của các phương pháp điều trị mới đối với NAFLD."} +{"idx": 1890055, "en": "Large bone injuries, defects, and chronic wounds present a major problem for medicine. Several therapeutic strategies are used clinically to precipitate bone including a combination therapy delivering osteoinductive bone morphogenetic protein 2 (rhBMP-2) via an osteoconductive scaffold (absorbable collagen sponge [ACS], i.e., INFUSE). Adverse side effects reportedly associated with rhBMP2 administration include rampant inflammation and clinical failures. Although acute inflammation is necessary for proper healing in bone, inflammatory cascade dysregulation can result in sustained tissue damage and poor healing. We hypothesized that a subclinical dose of rhBMP2 modeled in the murine calvarial defect would not precipitate alterations to inflammatory markers during acute phases of bone wound healing. We utilized the 5 mm critical size calvarial defect in C57BL6 wild-type mice which were subsequently treated with ACS and a subclinical dose of rhBMP2 shown to be optimal for healing. Three and 7-day postoperative time points were used to assess the role that rhBMP-2 plays in modulating inflammation vs. ACS alone by cytokine array and histological interrogation. Data revealed that rhBMP-2 delivery resulted in substantial modulation of several markers associated with inflammation, most of which decreased to levels similar to control by the 7-day time point. Additionally, while rhBMP-2 administration increased macrophage response, this peptide had a little noticeable effect on traditional markers of macrophage polarization (M1-iNOS, M2-Arg1). These results suggest that rhBMP-2 delivered at a lower dose does not precipitate rampant inflammation. Thus, an assessment of dosing for rhBMP-2 therapies may lead to better healing outcomes and less surgical failure.", "gemini": "Chấn thương xương lớn, khuyết tật và vết thương mãn tính là một vấn đề lớn đối với y học. Một số chiến lược điều trị được sử dụng lâm sàng để kết tủa xương bao gồm liệu pháp kết hợp cung cấp protein hình thành xương gây cảm ứng xương 2 (rhBMP-2) thông qua giá đỡ dẫn xương (miếng bọt biển collagen có thể hấp thụ [ACS], tức là INFUSE). Các tác dụng phụ có hại được báo cáo liên quan đến việc sử dụng rhBMP2 bao gồm tình trạng viêm tràn lan và thất bại lâm sàng. Mặc dù tình trạng viêm cấp tính là cần thiết để chữa lành xương đúng cách, nhưng sự rối loạn của quá trình viêm có thể dẫn đến tổn thương mô kéo dài và lành kém. Chúng tôi giả định rằng một liều rhBMP2 dưới lâm sàng được mô hình hóa trong khuyết tật xương sọ ở chuột sẽ không làm thay đổi các dấu hiệu viêm trong giai đoạn cấp tính của quá trình lành vết thương xương. Chúng tôi đã sử dụng khuyết tật xương sọ có kích thước tới hạn 5 mm ở chuột C57BL6 kiểu hoang dã, sau đó được điều trị bằng ACS và một liều rhBMP2 dưới lâm sàng được chứng minh là tối ưu để chữa lành. Các thời điểm sau phẫu thuật 3 và 7 ngày được sử dụng để đánh giá vai trò của rhBMP-2 trong việc điều chỉnh tình trạng viêm so với riêng ACS bằng mảng cytokine và thẩm vấn mô học. Dữ liệu cho thấy việc cung cấp rhBMP-2 dẫn đến sự điều chỉnh đáng kể một số dấu hiệu liên quan đến tình trạng viêm, hầu hết đều giảm xuống mức tương tự như đối chứng vào thời điểm 7 ngày. Ngoài ra, trong khi việc sử dụng rhBMP-2 làm tăng đáp ứng của đại thực bào, thì peptide này có tác dụng ít đáng chú ý đối với các dấu hiệu phân cực đại thực bào truyền thống (M1-iNOS, M2-Arg1). Những kết quả này cho thấy rằng rhBMP-2 được cung cấp ở liều thấp hơn không gây ra tình trạng viêm tràn lan. Do đó, việc đánh giá liều lượng cho các liệu pháp rhBMP-2 có thể dẫn đến kết quả chữa lành tốt hơn và ít thất bại trong phẫu thuật hơn."} +{"idx": 1890056, "en": "Long noncoding RNAs (lncRNAs) played an important role in tumorigenesis and development of hepatocellular carcinoma (HCC). In this study, we first demonstrated that lncRNA DLX6 antisense RNA 1 (DLX6-AS1) was upregulated in cancer tissues and cells lines compared with normal adjacent and cell line. Knock-down DLX6-AS1 by transfection with small interfering RNA (siRNA) suppressed cell proliferation, migration, and invasion of HCC cells. Cell cycle analysis showed that cells transfected with siRNA were arrested in G0/G1 phase. Then, we performed dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay to show that DLX6-AS1 could bind with miR-424-5p. And cotransfection inhibitor of miR-424-5p with siRNA of DLX6-AS1 could abolish the inhibitory effect of siRNA of DLX6-AS1 on cell proliferation, migration, and invasion. Moreover, we further demonstrated that the oncogene WEE1 G2 checkpoint kinase (WEE1) was the target of miR-424-5p and expression levels of WEE1 were positive correlation with that of DLX6-AS1. Taken together, these results suggested that upregulated DLX6-AS1 promoted cell proliferation, migration, and invasion of HCC through increasing expression of WEE1 via targeting miR-424-5p.", "gemini": "Các RNA không mã hóa dài (lncRNA) đóng một vai trò quan trọng trong quá trình hình thành khối u và phát triển ung thư biểu mô tế bào gan (HCC). Trong nghiên cứu này, đầu tiên chúng tôi chứng minh rằng lncRNA DLX6 antisense RNA 1 (DLX6-AS1) được tăng điều hòa ở các mô ung thư và dòng tế bào so với tế bào bình thường liền kề và dòng tế bào. DLX6-AS1 hạ gục bằng cách chuyển gen với RNA can thiệp nhỏ (siRNA) ức chế sự tăng sinh tế bào, di chuyển và xâm lấn của tế bào HCC. Phân tích chu kỳ tế bào cho thấy các tế bào được chuyển gen siRNA đã bị bắt giữ ở pha G0/G1. Sau đó, chúng tôi đã thực hiện xét nghiệm báo cáo kép luciferase và xét nghiệm kết tủa miễn dịch RNA (RIP) để chứng minh rằng DLX6-AS1 có thể liên kết với miR-424-5p. Và chất ức chế đồng chuyển gen của miR-424-5p với siRNA của DLX6-AS1 có thể xóa bỏ tác dụng ức chế của siRNA của DLX6-AS1 đối với sự tăng sinh, di chuyển và xâm lấn tế bào. Hơn nữa, chúng tôi chứng minh thêm rằng gen gây ung thư WEE1 G2 checkpoint kinase (WEE1) là mục tiêu của miR-424-5p và mức biểu hiện của WEE1 có mối tương quan thuận với DLX6-AS1. Tổng hợp lại, những kết quả này cho thấy DLX6-AS1 tăng điều hòa thúc đẩy sự tăng sinh, di chuyển và xâm lấn tế bào của HCC thông qua việc tăng biểu hiện của WEE1 bằng cách nhắm mục tiêu miR-424-5p."} +{"idx": 1890057, "en": "A novel noncentrosymmetric (NCS) polar fluoride sulfate, CsSbF2 SO4 , was obtained by ionothermal synthesis. A meticulously designed co-substitution approach was used to successfully replace the [TiO6 ]8- and [PO4 ]3- functional groups in KTiOPO4 (KTP) with [SbO4 F2 ]7- and [SO4 ]2- units, respectively. The structure of CsSbF2 SO4 features a pseudo-3D framework consisting of interconnected 1D [SbF2 O2 SO4 ]5- chains of corner-sharing [SbO4 F2 ]7- octahedra and [SO4 ]2- tetrahedra. The title compound exhibits a sharply enlarged band gap compared to its parent compound, KTP, benefitting from the introduction of F- ions and the displacement of Sb3+ cations. Second harmonic generation (SHG) measurements manifested that CsSbF2 SO4 is phase-matchable and revealed a strong SHG response of about 3.0 KH2 PO4 (KDP), which is the highest value reported for any metal sulfate reported to date. The reported fluoride sulfate is a promising near ultraviolet (UV) nonlinear optical (NLO) material.", "gemini": "Một loại sunfat florua không tâm đối xứng (NCS) mới, CsSbF2 SO4 , đã được tổng hợp bằng phương pháp tổng hợp ion nhiệt. Một phương pháp đồng thế được thiết kế tỉ mỉ đã được sử dụng để thay thế thành công các nhóm chức [TiO6 ]8- và [PO4 ]3- trong KTiOPO4 (KTP) bằng các đơn vị [SbO4 F2 ]7- và [SO4 ]2-, tương ứng. Cấu trúc của CsSbF2 SO4 có đặc điểm là một khung giả 3D bao gồm các chuỗi [SbF2 O2 SO4 ]5- 1D được kết nối với nhau của các bát diện [SbO4 F2 ]7- chia sẻ góc và các tứ diện [SO4 ]2-. Hợp chất tiêu đề thể hiện một khoảng cách băng tần mở rộng mạnh so với hợp chất gốc của nó, KTP, được hưởng lợi từ sự đưa vào của các ion F- và sự dịch chuyển của các cation Sb3+. Các phép đo sinh thế hệ sóng hài thứ hai (SHG) biểu hiện rằng CsSbF2 SO4 có thể ghép pha và tiết lộ phản ứng SHG mạnh khoảng 3,0 KH2 PO4 (KDP), đây là giá trị cao nhất được báo cáo cho bất kỳ sunfat kim loại nào được báo cáo cho đến nay. Sunfat florua được báo cáo là một vật liệu quang học phi tuyến tính (NLO) gần cực tím (UV) đầy hứa hẹn."} +{"idx": 1890058, "en": "Remodeling of the gene regulatory network in cells is believed to be a prerequisite for their lineage reprogramming. However, its key regulatory factors are not yet elucidated. In this article, we investigate the role of PIWI proteins and provide evidence that one of them, MIWI2, is elicited during transdifferentiation of fibroblasts into hepatocyte-like cells. In coincidence with the peak expression of MIWI2, we identified the appearance of a unique intermediate epigenetic state characterized by a specific Piwi-interacting RNA (piRNA) profile consisting of 219 novel sequences. Knockout of MIWI2 greatly improved the formation of the induced hepatocytes, whereas overexpression of exogenous MIWI2 completely abolished the stimulated effect. A bioinformatics analysis of piRNA interaction network, followed by experimental validation, revealed the Notch signaling pathway as one of the immediate effectors of MIWI2. Altogether, our results show for the first time that temporal expression of MIWI2 contributes negatively to cell plasticity not only in germline, but also in developed cells, such as mouse fibroblasts. Stem Cells 2019;37:803-812.", "gemini": "Việc tái tạo mạng lưới điều hòa gen trong các tế bào được cho là điều kiện tiên quyết để lập trình lại dòng tế bào của chúng. Tuy nhiên, các yếu tố điều hòa chính của nó vẫn chưa được làm sáng tỏ. Trong bài viết này, chúng tôi nghiên cứu vai trò của protein PIWI và đưa ra bằng chứng rằng một trong số chúng, MIWI2, được tạo ra trong quá trình chuyển hóa tế bào sợi thành tế bào giống tế bào gan. Trùng với thời điểm biểu hiện đỉnh của MIWI2, chúng tôi đã xác định được sự xuất hiện của một trạng thái biểu sinh trung gian độc đáo được đặc trưng bởi một cấu hình RNA tương tác với Piwi (piRNA) cụ thể bao gồm 219 trình tự mới. Việc loại bỏ MIWI2 đã cải thiện đáng kể sự hình thành của các tế bào gan được cảm ứng, trong khi việc biểu hiện quá mức MIWI2 ngoại sinh đã loại bỏ hoàn toàn tác dụng kích thích. Phân tích tin sinh học về mạng tương tác piRNA, tiếp theo là xác nhận thực nghiệm, đã tiết lộ con đường truyền tín hiệu Notch là một trong những tác nhân gây ra MIWI2 ngay lập tức. Nhìn chung, kết quả của chúng tôi cho thấy lần đầu tiên rằng biểu hiện tạm thời của MIWI2 góp phần tiêu cực vào tính dẻo của tế bào không chỉ ở dòng mầm mà còn ở các tế bào đã phát triển, chẳng hạn như tế bào sợi chuột. Tế bào gốc 2019; 37: 803-812."} +{"idx": 1890059, "en": "Herein, we introduce an additive-free visible-light-induced Passerini multicomponent polymerization (MCP) for the generation of high molar mass chains. In place of classical aldehydes (or ketones), highly reactive, in situ photogenerated thioaldehydes are exploited along with isocyanides and carboxylic acids. Prone to side reactions, the thioaldehyde moieties create a complex reaction environment which can be tamed by optimizing the synthetic conditions utilizing stochastic reaction path analysis, highlighting the potential of semi-batch procedures. Once the complex MCP environment is understood, step-growth polymers can be synthesized under mild reaction conditions which-after a Mumm rearrangement-result in the incorporation of thioester moieties directly into the polymer backbone, leading to soft matter materials that can be degraded by straightforward aminolysis or chain expanded by thiirane insertion.", "gemini": "Ở đây, chúng tôi giới thiệu một quá trình trùng hợp đa thành phần Passerini (MCP) không có chất phụ gia được kích thích bằng ánh sáng khả kiến để tạo ra các chuỗi khối lượng mol cao. Thay thế các aldehyd cổ điển (hoặc xeton), các thioaldehyd phản ứng cao được tạo ra tại chỗ được khai thác cùng với các isocyanid và axit cacboxylic. Dễ xảy ra phản ứng phụ, các nhóm thioaldehyd tạo ra một môi trường phản ứng phức tạp có thể được thuần hóa bằng cách tối ưu hóa các điều kiện tổng hợp sử dụng phân tích đường dẫn phản ứng ngẫu nhiên, làm nổi bật tiềm năng của các quy trình bán mẻ. Khi môi trường MCP phức tạp được hiểu, các polyme tăng trưởng theo bước có thể được tổng hợp trong điều kiện phản ứng nhẹ - sau khi sắp xếp lại Mumm - dẫn đến sự kết hợp của các nhóm thioester trực tiếp vào xương sống polyme, dẫn đến các vật liệu vật chất mềm có thể bị phân hủy bằng aminolysis đơn giản hoặc mở rộng chuỗi bằng cách chèn thiiran."} +{"idx": 1890060, "en": "BACKGROUND Computer vision syndrome is common and affects performance of visual tasks. Background illumination, light source, light compensation, position of the display, contrast and glare are environmental factors associated with computer vision syndrome. The aim of this study is to investigate the effects of reflected glare and visual field lighting on computer vision syndrome. METHODS In a reflected glare experiment, participants performed a two-hour visual task using a glossy, matte, or glare-free surface display in two visual environments (normal, glare). In a visual field lighting experiment, participants performed the visual task in dim lighting, uneven supplementary lighting, or uniform supplementary lighting. Visual function parameters, including critical fusion frequency, heterophoria, amplitude of accommodation and accommodative facility were evaluated by the investigators and a visual fatigue questionnaire was completed before and after the visual task. Visual performance was also recorded. In addition, the variation of pupil size under different lighting conditions was analysed. RESULTS Critical fusion frequency was the only visual function parameter which decreased significantly after the visual task. The questionnaire score was significantly higher in a glare environment and was lower when the task was performed using a glare-free display. Visual performance was significantly worse in the glossy display group. The increment in the questionnaire score was smaller in the uniform supplementary lighting group. Visual performance was significantly worse in the dim lighting or uneven supplementary lighting group, but not in the uniform supplementary lighting group. Variation in pupil size was significantly greater in the dim lighting condition than in the supplementary lighting condition. CONCLUSION Critical fusion frequency is an effective indicator of computer vision syndrome. Glare-free displays could alleviate visual fatigue and preserve visual performance. Uniform supplementary lighting could decrease variation in pupil size and prevent eye strain.", "gemini": "BỐI CẢNH Hội chứng thị giác máy tính là phổ biến và ảnh hưởng đến hiệu suất của các nhiệm vụ thị giác. Độ sáng nền, nguồn sáng, bù sáng, vị trí màn hình, độ tương phản và độ chói là các yếu tố môi trường liên quan đến hội chứng thị giác máy tính. Mục đích của nghiên cứu này là để điều tra các tác động của ánh sáng chói phản xạ và ánh sáng trường thị giác đến hội chứng thị giác máy tính. PHƯƠNG PHÁP Trong một thí nghiệm về ánh sáng chói phản xạ, những người tham gia đã thực hiện một nhiệm vụ thị giác trong hai giờ sử dụng màn hình bề mặt bóng, mờ hoặc không chói trong hai môi trường thị giác (bình thường, chói). Trong một thí nghiệm về ánh sáng trường thị giác, những người tham gia đã thực hiện nhiệm vụ thị giác trong điều kiện ánh sáng yếu, ánh sáng bổ sung không đều hoặc ánh sáng bổ sung đồng đều. Các thông số chức năng thị giác, bao gồm tần số hợp nhất tới hạn, dị hướng, biên độ điều tiết và khả năng điều tiết được các nhà nghiên cứu đánh giá và một bảng câu hỏi về mệt mỏi thị giác được hoàn thành trước và sau nhiệm vụ thị giác. Hiệu suất thị giác cũng được ghi lại. Ngoài ra, sự thay đổi kích thước đồng tử trong các điều kiện ánh sáng khác nhau đã được phân tích. KẾT QUẢ Tần số hợp nhất tới hạn là thông số chức năng thị giác duy nhất giảm đáng kể sau nhiệm vụ thị giác. Điểm số bảng câu hỏi cao hơn đáng kể trong môi trường chói và thấp hơn khi nhiệm vụ được thực hiện bằng màn hình không chói. Hiệu suất thị giác kém hơn đáng kể trong nhóm màn hình bóng. Sự gia tăng điểm số bảng câu hỏi nhỏ hơn ở nhóm ánh sáng bổ sung đồng đều. Hiệu suất thị giác kém hơn đáng kể trong nhóm ánh sáng yếu hoặc nhóm ánh sáng bổ sung không đều, nhưng không phải trong nhóm ánh sáng bổ sung đồng đều. Sự thay đổi về kích thước đồng tử lớn hơn đáng kể trong điều kiện ánh sáng yếu so với điều kiện ánh sáng bổ sung. KẾT LUẬN Tần số hợp nhất tới hạn là một chỉ số hiệu quả của hội chứng thị giác máy tính. Màn hình không chói có thể làm giảm mệt mỏi thị giác và duy trì hiệu suất thị giác. Ánh sáng bổ sung đồng đều có thể làm giảm sự thay đổi kích thước đồng tử và ngăn ngừa mỏi mắt."} +{"idx": 1890061, "en": "Canine malignant melanoma (MM) is a highly aggressive tumour with a low survival rate and represents an ideal spontaneous model for the human counterpart. Considerable progress has been recently obtained, but the therapeutic success for canine melanoma is still challenging. Little is known about the mechanisms beyond pathogenesis and melanoma development, and the molecular response to radiotherapy has never been explored before. A faster and deeper understanding of cancer mutational processes and developing mechanisms are now possible through next generation sequencing technologies. In this study, we matched whole exome and transcriptome sequencing in four dogs affected by MM at diagnosis and at disease progression to identify possible genetic mechanisms associated with therapy failure. According to previous studies, a genetic similarity between canine MM and its human counterpart was observed. Several somatic mutations were functionally related to MAPK, PI3K/AKT and p53 signalling pathways, but located in genes other than BRAF, RAS and KIT. At disease progression, several mutations were related to therapy effects. Natural killer cell-mediated cytotoxicity and several immune-system-related pathways resulted activated opening a new scenario on the microenvironment in this tumour. In conclusion, this study suggests a potential role of the immune system associated to radiotherapy in canine melanoma, but a larger sample size associated with functional studies are needed.", "gemini": "U ác tính ở chó (MM) là một khối u rất hung dữ với tỷ lệ sống sót thấp và là một mô hình tự phát lý tưởng cho người tương ứng. Tiến bộ đáng kể đã đạt được gần đây, nhưng thành công điều trị đối với u ác tính ở chó vẫn còn nhiều thách thức. Người ta biết rất ít về các cơ chế vượt quá quá trình sinh bệnh và phát triển khối u ác tính, và phản ứng phân tử đối với xạ trị chưa bao giờ được khám phá trước đây. Hiểu nhanh hơn và sâu hơn về các quá trình đột biến ung thư và các cơ chế phát triển hiện có thể thông qua các công nghệ giải trình tự thế hệ tiếp theo. Trong nghiên cứu này, chúng tôi đã kết hợp toàn bộ exome và giải trình tự transcriptome ở bốn con chó bị MM khi chẩn đoán và khi bệnh tiến triển để xác định các cơ chế di truyền có thể liên quan đến thất bại của liệu pháp. Theo các nghiên cứu trước đây, người ta đã quan sát thấy sự giống nhau về mặt di truyền giữa MM ở chó và người tương ứng. Một số đột biến soma có liên quan về mặt chức năng đến các con đường truyền tín hiệu MAPK, PI3K/AKT và p53, nhưng nằm ở các gen khác với BRAF, RAS và KIT. Khi bệnh tiến triển, một số đột biến có liên quan đến tác dụng của liệu pháp. Tế bào tiêu diệt tự nhiên do tế bào giết tự nhiên trung gian và một số con đường liên quan đến hệ thống miễn dịch đã hoạt động mở ra một kịch bản mới về môi trường vi mô trong khối u này. Tóm lại, nghiên cứu này gợi ý một vai trò tiềm ẩn của hệ thống miễn dịch liên quan đến xạ trị trong u ác tính ở chó, nhưng cần có kích thước mẫu lớn hơn liên quan đến các nghiên cứu chức năng."} +{"idx": 1890062, "en": "BACKGROUND From an evolutionary biology perspective, where growth and reproduction trade-off against longevity, we assessed the associations of growth from birth to puberty by phase with later glycemic indicators and any differences by sex. METHODS In the population-representative Hong Kong Chinese \"Children of 1997\" birth cohort (n = 8327), the relation of initial size (weight-for-age z score (WAZ) at birth, length/height-for-age z score (LAZ) at 3 months or body-mass-index-for-age z score (BAZ) at 3 months based on the World Health Organization growth standards/references) and growth at different phases (WAZ gains from 0 to 2 and 2 to 8 years, LAZ or BAZ gains from 3 months to 3 years, 3 to 8 years and 8 to 14 years) with fasting plasma glucose (FPG) and glycated hemoglobin (HbA1c) at ~17.5 years, was assessed using adjusted partial least squares regression. Additional analyses further considered growth in late and early infancy. RESULTS This study included 3276 of the cohort participants. Higher WAZ gain from 2 to 8 years, LAZ and BAZ gains from 3 to 8 years were consistently associated with higher FPG, adjusted for maternal and infant characteristics, family history of diabetes and household income. Also, higher BAZ gain from 3 to 8 years was associated with higher HbA1c. These associations did not differ by sex. CONCLUSIONS Our findings suggest different mechanisms could underlie the pathogenesis of glucose intolerance. Factors that drive specific growth at different phases need to be evaluated to better inform child growth management for long-term health outcomes.", "gemini": "BỐI CẢNH Từ góc độ sinh học tiến hóa, nơi tăng trưởng và sinh sản đánh đổi với tuổi thọ, chúng tôi đã đánh giá mối liên quan của sự tăng trưởng từ khi sinh đến tuổi dậy thì theo từng giai đoạn với các chỉ số đường huyết sau này và bất kỳ sự khác biệt nào theo giới tính. PHƯƠNG PHÁP Trong nhóm đối chứng đại diện cho dân số là nhóm trẻ em Hồng Kông sinh năm 1997 (n = 8327), mối quan hệ giữa kích thước ban đầu (điểm chuẩn cân nặng theo tuổi (WAZ) khi sinh, điểm chuẩn chiều dài/chiều cao theo tuổi (LAZ) ở 3 tháng hoặc điểm chuẩn chỉ số khối cơ thể theo tuổi (BAZ) ở 3 tháng dựa trên các tiêu chuẩn/tham chiếu tăng trưởng của Tổ chức Y tế Thế giới) và tăng trưởng ở các giai đoạn khác nhau (tăng WAZ từ 0 đến 2 và 2 đến 8 năm, tăng LAZ hoặc BAZ từ 3 tháng đến 3 năm, 3 đến 8 năm và 8 đến 14 năm) với đường huyết lúc đói (FPG) và hemoglobin glycosyl hóa (HbA1c) ở ~17,5 năm, được đánh giá bằng hồi quy bình phương từng phần điều chỉnh. Các phân tích bổ sung xem xét thêm sự tăng trưởng ở giai đoạn đầu và cuối thời thơ ấu. KẾT QUẢ Nghiên cứu này bao gồm 3276 người tham gia nhóm đối chứng. Tăng WAZ cao hơn từ 2 đến 8 năm, tăng LAZ và BAZ từ 3 đến 8 năm luôn liên quan đến FPG cao hơn, được điều chỉnh theo các đặc điểm của mẹ và trẻ sơ sinh, tiền sử gia đình mắc bệnh tiểu đường và thu nhập hộ gia đình. Ngoài ra, tăng BAZ cao hơn từ 3 đến 8 năm có liên quan đến HbA1c cao hơn. Những mối liên quan này không khác nhau theo giới tính. KẾT LUẬN Các phát hiện của chúng tôi cho thấy các cơ chế khác nhau có thể là nguyên nhân gây ra bệnh lý không dung nạp glucose. Các yếu tố thúc đẩy sự tăng trưởng cụ thể ở các giai đoạn khác nhau cần được đánh giá để thông tin tốt hơn cho việc quản lý tăng trưởng của trẻ em nhằm đạt được kết quả sức khỏe lâu dài."} +{"idx": 1890063, "en": "A new sample preparation method based on SBA-15 assisted electromembrane extraction coupled with corona discharge ion mobility spectrometer was developed for the determination of Thiabendazole as a model basic pesticide in fruit juice samples. The addition of SBA-15 in the supported liquid membrane in electromembrane extraction system not only can lead to enhancement of the effective surface area, but also introducing the negatively charged silanol groups into supported liquid membrane might improve migration of positively charged analytes toward the supported liquid membrane and finally into the acceptor solution. To investigate the effect of the presence of SBA-15 in the supported liquid membrane on the extraction efficiency, a comparative study was carried out between the conventional electromembrane extraction and SBA-15/electromembrane extraction methods. Under the optimized conditions, SBA-15/electromembrane extraction method showed higher extraction efficiencies in comparison with conventional electromembrane extraction method. SBA-15/electromembrane extraction method exhibited a low limit of detection (0.9 ng/mL), high preconcentration factor (167) and high recovery (83%). Finally, the applicability of SBA-15/electromembrane extraction method was studied by the extraction and determination of Thiabendazole as a model basic pesticide in fruit juice samples.", "gemini": "Một phương pháp chuẩn bị mẫu mới dựa trên phương pháp chiết xuất màng điện hỗ trợ SBA-15 kết hợp với phổ kế độ linh động ion phóng điện vành được phát triển để xác định Thiabendazole như một loại thuốc trừ sâu cơ bản mẫu trong các mẫu nước ép trái cây. Việc bổ sung SBA-15 vào màng lỏng được hỗ trợ trong hệ thống chiết xuất màng điện không chỉ có thể dẫn đến tăng diện tích bề mặt hiệu quả mà còn đưa các nhóm silanol tích điện âm vào màng lỏng được hỗ trợ có thể cải thiện quá trình di chuyển các chất phân tích tích điện dương về phía màng lỏng được hỗ trợ và cuối cùng vào dung dịch nhận. Để nghiên cứu hiệu quả của sự hiện diện của SBA-15 trong màng lỏng được hỗ trợ đối với hiệu suất chiết xuất, một nghiên cứu so sánh đã được thực hiện giữa phương pháp chiết xuất màng điện thông thường và phương pháp chiết xuất màng điện/SBA-15. Trong các điều kiện tối ưu, phương pháp chiết xuất màng điện/SBA-15 cho thấy hiệu suất chiết xuất cao hơn so với phương pháp chiết xuất màng điện thông thường. Phương pháp chiết xuất màng điện/SBA-15 thể hiện giới hạn phát hiện thấp (0,9 ng/mL), hệ số tiền cô đặc cao (167) và độ thu hồi cao (83%). Cuối cùng, khả năng ứng dụng của phương pháp chiết xuất màng điện/SBA-15 đã được nghiên cứu bằng cách chiết xuất và xác định Thiabendazole như một loại thuốc trừ sâu cơ bản mẫu trong các mẫu nước ép trái cây."} +{"idx": 1890064, "en": "PURPOSE Vegetation size is a prognostic predictor in infective endocarditis (IE) and guides surgical management. The aim of this study was to evaluate the accuracy of real-time 3-dimensional transesophageal echocardiography (RT3DTEE) compared to 2DTEE in the diagnosis and characterization of vegetation, as well as its potential clinical impact. METHODS Two hundred and three consecutive patients with IE were recruited (2009-2016) and retrospectively analyzed. Vegetation diameters and area from 68 patients were measured by 2DTEE and RT3DTEE at admission. The association between size and systemic embolisms was evaluated with logistic regression models. Differences in the discriminative power for the best dimensions' cutoff points were assessed by comparing the area under the ROC curves (AUC). RESULTS Vegetation size and area were larger by RT3DTEE (P < 0.001) than by 2DTEE, and RT3DTEE was especially relevant in the characterization of nonfiliform vegetation, Morphology was strongly associated with friability, being sessile vegetation less likely to embolize, compared to filiform and raceme-shaped ones (15.4% vs 46% vs 50%). Major diameter by RT3DTEE had better embolic predictive performance than 2DTEE (AUC 0.76 [0.57-0.89] vs 0.71 [0.53-0.86]; P = 0.611). The best cutoff points associated with embolic events during the infection were 17 mm for RT3DTEE and 15 mm for 2DTEE. Based exclusively on vegetation size, the proportion of patients meeting a surgical indication according to current guidelines is higher using RT3DTEE. CONCLUSIONS RT3DTEE allows a better characterization of IE vegetation than 2DTEE, what may have a clinical impact on surgical management and also prognostic due to a more accurate prediction of embolic risk.", "gemini": "MỤC ĐÍCH Kích thước thực vật là một yếu tố tiên lượng trong viêm nội tâm mạc nhiễm trùng (IE) và hướng dẫn việc xử trí phẫu thuật. Mục đích của nghiên cứu này là đánh giá độ chính xác của siêu âm tim qua thực quản 3 chiều thời gian thực (RT3DTEE) so với siêu âm tim qua thực quản 2D (2DTEE) trong chẩn đoán và đặc điểm của thực vật, cũng như tác động lâm sàng tiềm tàng của nó. PHƯƠNG PHÁP Hai trăm ba bệnh nhân liên tiếp mắc IE đã được tuyển dụng (2009-2016) và được phân tích hồi cứu. Đường kính và diện tích thực vật từ 68 bệnh nhân được đo bằng 2DTEE và RT3DTEE khi nhập viện. Sự liên quan giữa kích thước và thuyên tắc toàn thân được đánh giá bằng mô hình hồi quy logistic. Sự khác biệt về khả năng phân biệt đối với các điểm cắt kích thước tốt nhất được đánh giá bằng cách so sánh diện tích dưới đường cong ROC (AUC). KẾT QUẢ Kích thước và diện tích thực vật lớn hơn theo RT3DTEE (P < 0,001) so với 2DTEE, và RT3DTEE đặc biệt có liên quan trong đặc điểm của thực vật không phải dạng sợi. Hình thái có liên quan chặt chẽ với độ dễ vỡ, thực vật ít có khả năng gây thuyên tắc, so với thực vật dạng sợi và dạng chùm nho (15,4% so với 46% so với 50%). Đường kính chính theo RT3DTEE có hiệu suất dự đoán thuyên tắc tốt hơn 2DTEE (AUC 0,76 [0,57-0,89] so với 0,71 [0,53-0,86]; P = 0,611). Các điểm cắt tốt nhất liên quan đến các biến cố thuyên tắc trong quá trình nhiễm trùng là 17 mm đối với RT3DTEE và 15 mm đối với 2DTEE. Chỉ dựa trên kích thước thực vật, tỷ lệ bệnh nhân đáp ứng chỉ định phẫu thuật theo hướng dẫn hiện hành cao hơn khi sử dụng RT3DTEE. KẾT LUẬN RT3DTEE cho phép đặc điểm thực vật IE tốt hơn 2DTEE, có thể có tác động lâm sàng đến việc xử trí phẫu thuật và cũng tiên lượng do dự đoán chính xác hơn nguy cơ thuyên tắc."} +{"idx": 1890065, "en": "Notoginsenoside Fc, which is a protopanaxdiol-type saponin isolated from the leaves of Panax notoginseng, exhibits an exceptional antiplatelet aggregatory effect. To study the modulating effect of gastrointestinal contents on the metabolic profile and pharmacokinetics, pseudo germ-free rats were used to study the influence of the bacterial community structure on the metabolic profile. Glycosidase activities were measured using the spectrophotometric method. Biotransformations of notoginsenoside Fc in normal and pseudo germ-free rat intestinal microflora were systematically investigated using ultra high performance liquid chromatography with tandem quadrupole/time-of-flight mass spectrometry. Moreover, a liquid chromatography with tandem mass spectrometry method was established for simultaneous determination of the notoginsenoside Fc prototype and its degradation products. Through an in vivo pharmacokinetic study, the pharmacokinetic characteristics were compared between normal rats and pseudo germ-free rats. During the in vitro biotransformation, seven deglycosylated products were detected and identified after incubation in the intestinal bacteria of normal rats. In pseudo germ-free rats, glycosidase activities were significantly decreased, and no obvious degradation occurred. In an in vivo study, the systemic exposure was significantly increased 40%, as evidenced by the area under the blood concentration-time curve from time zero to infinity value and half-life value, which were prolonged more in the pseudo germ-free group than in normal rats. The results demonstrate that patients who use intestinal bacteria-metabolized herbs, such as panax notoginseng, should understand the profile of intestinal bacteria to ensure therapeutic efficacy.", "gemini": "Notoginsenoside Fc, một saponin loại protopanaxdiol được phân lập từ lá của Panax notoginseng, thể hiện tác dụng ức chế kết tập tiểu cầu đặc biệt. Để nghiên cứu hiệu quả điều chỉnh của các thành phần trong đường tiêu hóa đối với cấu hình chuyển hóa và dược động học, các con chuột không có vi trùng giả đã được sử dụng để nghiên cứu ảnh hưởng của cấu trúc quần thể vi khuẩn đối với cấu hình chuyển hóa. Các hoạt động của glycosidase được đo bằng phương pháp quang phổ. Các quá trình chuyển hóa sinh học của notoginsenoside Fc trong hệ vi sinh đường ruột ở chuột bình thường và không có vi trùng giả đã được nghiên cứu một cách có hệ thống bằng cách sử dụng sắc ký lỏng hiệu năng cực cao với phổ khối tứ cực/thời gian bay song song. Hơn nữa, một phương pháp sắc ký lỏng với phổ khối song song đã được thiết lập để xác định đồng thời nguyên mẫu notoginsenoside Fc và các sản phẩm phân hủy của nó. Thông qua một nghiên cứu dược động học in vivo, các đặc tính dược động học đã được so sánh giữa chuột bình thường và chuột không có vi trùng giả. Trong quá trình chuyển hóa sinh học in vitro, bảy sản phẩm khử glycosyl đã được phát hiện và xác định sau khi ủ trong vi khuẩn đường ruột của chuột bình thường. Ở chuột không có vi trùng giả, hoạt động của glycosidase giảm đáng kể và không xảy ra quá trình phân hủy rõ ràng. Trong một nghiên cứu in vivo, sự phơi nhiễm toàn thân tăng đáng kể 40%, bằng chứng là diện tích dưới đường cong nồng độ trong máu theo thời gian từ thời điểm bằng không đến giá trị vô cực và giá trị thời gian bán hủy, kéo dài hơn ở nhóm không có vi trùng giả so với chuột bình thường. Kết quả chứng minh rằng những bệnh nhân sử dụng các loại thảo dược được chuyển hóa bởi vi khuẩn đường ruột, chẳng hạn như panax notoginseng, nên hiểu cấu hình của vi khuẩn đường ruột để đảm bảo hiệu quả điều trị."} +{"idx": 1890066, "en": "Worldwide, a growing burden of health and social issues now affect young people. Interagency collaboration and the \"integration\" of health and social care services are advocated to address the increasingly complex needs of at-risk youth and to reduce barriers to accessing care. In New Zealand, Youth-One-Stop-Shops (YOSSs) provide integrated health and social care to young people with complex needs. Little is known about how YOSSs facilitate collaborative care. This study explored the collaboration between YOSSs and external agencies between 2015 and 2017 using a multiple case study method. This paper reports qualitative focus group and individual interview data from two of four case sites including six YOSS staff and 14 external agency staff. Results showed participants regarded collaboration as critical to the successful care of high needs young people and were positive about working together. They believed YOSSs provided effective wraparound collaborative care and actively facilitated communication between diverse agencies on behalf of young people. The main challenges participants faced when working together related to the different \"world views\" and cultures of agencies which can run contrary to collaborative practice. Despite this, some highly collaborative relationships were apparent and staff in the different agencies perceived YOSSs had a lead role in co-ordinating collaborative care and were genuinely valued and trusted. However without the YOSS involvement, collaboration between agencies in relation to young people was less frequent and rarely went beyond limited information exchange. Establishing and maintaining trusting interpersonal relationships with individual staff was key to successfully negotiating agency differences. The study confirms that collaboration when caring for young people with high needs is complex and challenging, yet agencies from diverse sectors value collaboration and see the YOSS integrated wraparound approach as an important model of care.", "gemini": "Trên toàn thế giới, gánh nặng về các vấn đề sức khỏe và xã hội ngày càng gia tăng hiện nay đang ảnh hưởng đến những người trẻ tuổi. Sự hợp tác giữa các cơ quan và \"hội nhập\" các dịch vụ chăm sóc sức khỏe và xã hội được ủng hộ để giải quyết các nhu cầu ngày càng phức tạp của những thanh thiếu niên có nguy cơ và giảm các rào cản trong việc tiếp cận dịch vụ chăm sóc. Tại New Zealand, các Trung tâm dành cho thanh thiếu niên (YOSS) cung cấp dịch vụ chăm sóc sức khỏe và xã hội tích hợp cho những thanh thiếu niên có nhu cầu phức tạp. Người ta biết rất ít về cách các YOSS tạo điều kiện cho việc chăm sóc cộng tác. Nghiên cứu này đã khám phá sự hợp tác giữa các YOSS và các cơ quan bên ngoài trong giai đoạn 2015 đến 2017 bằng cách sử dụng phương pháp nghiên cứu nhiều trường hợp. Bài báo này báo cáo dữ liệu phỏng vấn nhóm tập trung định tính và dữ liệu phỏng vấn cá nhân từ hai trong số bốn địa điểm nghiên cứu trường hợp bao gồm sáu nhân viên YOSS và 14 nhân viên cơ quan bên ngoài. Kết quả cho thấy những người tham gia coi sự hợp tác là rất quan trọng đối với việc chăm sóc thành công những thanh thiếu niên có nhu cầu cao và có thái độ tích cực về việc làm việc cùng nhau. Họ tin rằng các YOSS đã cung cấp dịch vụ chăm sóc cộng tác toàn diện hiệu quả và chủ động tạo điều kiện thuận lợi cho việc giao tiếp giữa các cơ quan khác nhau thay mặt cho những người trẻ tuổi. Những thách thức chính mà những người tham gia phải đối mặt khi làm việc cùng nhau liên quan đến các \"quan điểm thế giới\" và nền văn hóa khác nhau của các cơ quan, có thể trái ngược với hoạt động hợp tác. Mặc dù vậy, một số mối quan hệ hợp tác chặt chẽ đã được thể hiện rõ ràng và các nhân viên trong các cơ quan khác nhau nhận thấy rằng các YOSS có vai trò chính trong việc phối hợp chăm sóc cộng tác và thực sự được coi trọng và tin tưởng. Tuy nhiên, nếu không có sự tham gia của YOSS, thì sự hợp tác giữa các cơ quan liên quan đến những người trẻ tuổi sẽ ít thường xuyên hơn và hiếm khi vượt ra ngoài việc trao đổi thông tin hạn chế. Việc thiết lập và duy trì các mối quan hệ giữa các cá nhân dựa trên sự tin tưởng với từng nhân viên là chìa khóa để đàm phán thành công các khác biệt của cơ quan. Nghiên cứu khẳng định rằng sự hợp tác khi chăm sóc những người trẻ tuổi có nhu cầu cao là phức tạp và đầy thách thức, nhưng các cơ quan từ nhiều lĩnh vực khác nhau coi trọng sự hợp tác và coi cách tiếp cận toàn diện tích hợp của YOSS là một mô hình chăm sóc quan trọng."} +{"idx": 1890067, "en": "AIM Pregnancy with myasthenia gravis (MG) is known to be associated with an increased cesarean section rate, presumably due to maternal fatigue during labor. Although epidural labor analgesia (ELA) appears to be a good option for circumventing maternal fatigue, a protocol for managing MG deliveries has not been established. This study, based on a review of our case series, aimed to evaluate the validity of our management protocol for maternal MG, in which ELA is used depending on MG severity. METHODS Parturients with systemic muscle weakness or worsening symptoms were classified as Category A (A), and those without symptoms were classified as Category B (B). In A, ELA was given at the onset of labor. Immediate vacuum delivery was done once the fetal head descended to station +2. For B, spontaneous vaginal delivery was chosen. The duration, blood loss, fetal weight, Apgar score and MG symptoms on post-partum day (PPD) 1, 14 and 30 were recorded. RESULTS Six patients were enrolled. Four were classified in A, and two were classified in B. No adverse events occurred during labor. Transvaginal delivery was successfully achieved in all the patients. Symptoms of MG were well-controlled. MG symptoms were stable on PPD 1 in all the patients although two patients complained of worsening symptoms after PPD 14. CONCLUSION Women with MG can safely undergo spontaneous or operative vaginal delivery. ELA is a good option for circumventing the effects of maternal fatigue on delivery. Our protocol may lower the cesarean section rate in maternal MG.", "gemini": "MỤC TIÊU Mang thai với bệnh nhược cơ (MG) được biết là có liên quan đến tỷ lệ sinh mổ tăng, có lẽ là do mẹ mệt mỏi trong quá trình chuyển dạ. Mặc dù giảm đau chuyển dạ ngoài màng cứng (ELA) dường như là một lựa chọn tốt để tránh tình trạng mệt mỏi của mẹ, nhưng vẫn chưa có phác đồ quản lý việc sinh ở những phụ nữ mắc MG. Nghiên cứu này, dựa trên việc xem xét loạt ca bệnh của chúng tôi, nhằm đánh giá tính hợp lệ của phác đồ quản lý MG của mẹ chúng tôi, trong đó ELA được sử dụng tùy thuộc vào mức độ nghiêm trọng của MG. PHƯƠNG PHÁP Những sản phụ có cơ yếu toàn thân hoặc các triệu chứng xấu đi được phân loại là Nhóm A (A), và những sản phụ không có triệu chứng được phân loại là Nhóm B (B). Trong nhóm A, ELA được dùng khi bắt đầu chuyển dạ. Đẻ chân không ngay lập tức được thực hiện khi đầu thai nhi hạ xuống trạm +2. Đối với nhóm B, sinh tự nhiên qua ngả âm đạo được lựa chọn. Thời gian, mất máu, cân nặng của thai nhi, điểm Apgar và các triệu chứng MG vào ngày sau sinh (PPD) 1, 14 và 30 được ghi lại. KẾT QUẢ Có sáu bệnh nhân được ghi danh. Bốn bệnh nhân được phân loại vào nhóm A và hai bệnh nhân được phân loại vào nhóm B. Không có biến cố bất lợi nào xảy ra trong quá trình chuyển dạ. Tất cả các bệnh nhân đều sinh ngả âm đạo thành công. Các triệu chứng của MG được kiểm soát tốt. Các triệu chứng MG ổn định vào PPD 1 ở tất cả các bệnh nhân mặc dù hai bệnh nhân phàn nàn về các triệu chứng xấu đi sau PPD 14. KẾT LUẬN Những phụ nữ mắc MG có thể sinh ngả âm đạo tự nhiên hoặc phẫu thuật một cách an toàn. ELA là một lựa chọn tốt để tránh những ảnh hưởng của tình trạng mệt mỏi của mẹ khi sinh con. Phác đồ của chúng tôi có thể làm giảm tỷ lệ sinh mổ ở những bà mẹ mắc MG."} +{"idx": 1890068, "en": "OBJECTIVES To evaluate the preventive effect of an insulin-like growth factor 1 sustained-release collagen urethral catheter on urethral stricture after urethral injury in a rabbit model. METHODS We made urethral catheters coated either with insulin-like growth factor 1 impregnated collagen or with only collagen, and we divided 19 male Japanese white rabbits into three groups according to the kind of catheter inserted immediately after the rabbit's urethra was injured by electrocoagulation. Group 1 (n = 7) had a catheter coated with insulin-like growth factor 1 impregnated collagen inserted; group 2 (n = 7) had a catheter coated with only collagen inserted; and group 3 (n = 5) had an uncoated catheter inserted. A total of 14 days later, the injured urethras were evaluated by urethrography and urethroscopy, and were also histologically examined. RESULTS Urethrography showed that the ratio of the urethral lumen diameter in injured urethra to that in normal urethra was the largest in group 1 (P < 0.0001). In addition, five of the seven rabbits in group 1 (71.4%) had a urethral lumen large enough for passage of a urethroscope, a fraction larger than the corresponding fractions in groups 2 (57.1%) and 3 (20%). On histological analysis, the injured area not covered with regenerated urethral epithelium tended to be smaller in group 1 than the other two groups, but the mean difference was not significant (P = 0.19). CONCLUSIONS An insulin-like growth factor 1 sustained-release collagen urethral catheter significantly improves wound healing and prevents urethral stricture after urethral injury.", "gemini": "MỤC TIÊU Đánh giá tác dụng phòng ngừa của ống thông niệu đạo collagen giải phóng chậm yếu tố tăng trưởng giống insulin 1 đối với hẹp niệu đạo sau chấn thương niệu đạo ở mô hình thỏ. PHƯƠNG PHÁP Chúng tôi đã chế tạo các ống thông niệu đạo được phủ bằng collagen tẩm yếu tố tăng trưởng giống insulin 1 hoặc chỉ bằng collagen, và chúng tôi chia 19 con thỏ trắng Nhật Bản đực thành ba nhóm theo loại ống thông được đưa vào ngay sau khi niệu đạo của thỏ bị thương do điện đông. Nhóm 1 (n = 7) được đặt ống thông phủ collagen tẩm yếu tố tăng trưởng giống insulin 1; nhóm 2 (n = 7) được đặt ống thông chỉ phủ collagen; và nhóm 3 (n = 5) được đặt ống thông không phủ. Tổng cộng 14 ngày sau, các niệu đạo bị thương được đánh giá bằng chụp niệu đạo và nội soi niệu đạo, đồng thời cũng được kiểm tra mô học. KẾT QUẢ Chụp niệu đạo cho thấy tỷ lệ đường kính lòng niệu đạo ở niệu đạo bị thương so với niệu đạo bình thường lớn nhất ở nhóm 1 (P < 0,0001). Ngoài ra, năm trong số bảy con thỏ trong nhóm 1 (71,4%) có lòng niệu đạo đủ lớn để nội soi niệu đạo, tỷ lệ này lớn hơn tỷ lệ tương ứng ở nhóm 2 (57,1%) và nhóm 3 (20%). Về phân tích mô học, diện tích bị thương không được phủ bằng biểu mô niệu đạo tái tạo có xu hướng nhỏ hơn ở nhóm 1 so với hai nhóm còn lại, nhưng sự khác biệt trung bình không đáng kể (P = 0,19). KẾT LUẬN Ống thông niệu đạo collagen giải phóng chậm yếu tố tăng trưởng giống insulin 1 cải thiện đáng kể quá trình lành vết thương và ngăn ngừa hẹp niệu đạo sau chấn thương niệu đạo."} +{"idx": 1890069, "en": "BACKGROUND The aim was to assess factors influencing the timing of defunctioning stoma (DS) reversal following low anterior resection of rectum for cancer (LAR). METHODS All patients operated with LAR and a primary DS during a 9-year period were included. Reversal later than 120 days after LAR was considered as delayed. A DS not reversed within 2 years was considered as permanent. RESULTS In the present study, median age at LAR was 67 years, 45% were females, median body mass index was 25, 87% had American Society of Anesthesiologists class I or II, 64% had pre-operative radiotherapy, and 3% had cancer stage IV. A total of 79% (92/116) underwent stoma reversal, whereof 25% (23/92) were reversed within 120 days. The most common health-related reasons for delayed stoma reversal were adjuvant chemotherapy in 38%, symptomatic anastomotic leakage in 16% and other post-operative adverse events in 13%. In 35% delayed stoma reversal was because of low priority within the healthcare system. A total of 18% (20/110) never had their DS reversed (n = 11) or had their DS converted to a permanent end colostomy (n = 9). Major risk factors for permanent stoma were stage IV cancer in 55%, and symptomatic anastomotic leakage in 30%. CONCLUSION One fourth of the patients had their defunctioning stoma reversed within 120 days. The most common identifiable medical reasons for delayed stoma reversal were adjuvant chemotherapy and symptomatic anastomotic leakage, while in one out of three patients it was because of low priority by the healthcare provider.", "gemini": "BỐI CẢNH Mục đích là đánh giá các yếu tố ảnh hưởng đến thời điểm đảo ngược lỗ mở thông đại tràng (DS) sau khi cắt bỏ trực tràng thấp trước vì ung thư (LAR). PHƯƠNG PHÁP Tất cả bệnh nhân được phẫu thuật LAR và DS nguyên phát trong thời gian 9 năm đều được đưa vào nghiên cứu. Việc đảo ngược muộn hơn 120 ngày sau LAR được coi là chậm. DS không được đảo ngược trong vòng 2 năm được coi là vĩnh viễn. KẾT QUẢ Trong nghiên cứu hiện tại, tuổi trung bình lúc LAR là 67, 45% là nữ, chỉ số khối cơ thể trung bình là 25, 87% có Hội Gây mê Hoa Kỳ lớp I hoặc II, 64% được xạ trị trước phẫu thuật và 3% có ung thư giai đoạn IV. Tổng cộng 79% (92/116) đã đảo ngược lỗ mở thông, trong đó 25% (23/92) được đảo ngược trong vòng 120 ngày. Các lý do liên quan đến sức khỏe phổ biến nhất dẫn đến việc đảo ngược lỗ mở thông chậm là hóa trị bổ trợ ở 38%, rò nối có triệu chứng ở 16% và các biến cố bất lợi sau phẫu thuật khác ở 13%. Ở 35%, việc đảo ngược lỗ mở thông chậm là do mức độ ưu tiên thấp trong hệ thống chăm sóc sức khỏe. Tổng cộng 18% (20/110) không bao giờ đảo ngược DS của họ (n = 11) hoặc chuyển DS của họ thành hậu môn nhân tạo vĩnh viễn (n = 9). Các yếu tố nguy cơ chính gây ra lỗ mở thông vĩnh viễn là ung thư giai đoạn IV ở 55% và rò nối có triệu chứng ở 30%. KẾT LUẬN Một phần tư số bệnh nhân đã đảo ngược lỗ mở thông đại tràng trong vòng 120 ngày. Các lý do y tế phổ biến nhất có thể xác định được dẫn đến việc đảo ngược lỗ mở thông chậm là hóa trị bổ trợ và rò nối có triệu chứng, trong khi ở một trong ba bệnh nhân là do mức độ ưu tiên thấp của nhà cung cấp dịch vụ chăm sóc sức khỏe."} +{"idx": 1890070, "en": "Over the past decade, the potential harms regarding young people's use of technology have attracted mounting political, media and research attention worldwide. One practice engaged in by many young people is that of \"sexting\" and the sharing of partially, or complete nude images (\"selfies\"). Such images are not always retained within private spaces and are prone to be shared, with significant psychosocial consequences for young people involved. A significant risk is the hidden nature of some online interactions, with potential for grooming and child sexual exploitation. As key professionals working with young people, public health nurses have potential to educate and explore the risks with them. Yet to date, to our knowledge there has been no research in relation to public health nurses' understandings of the practices involved or their potential harms. A qualitative study was undertaken drawing theoretically on the common-sense model (CSM) to frame the analysis. Eighteen semi-structured interviews were conducted with public health nurses in a region of England in 2016. Data were analysed through thematic analysis, and mapped to the five domains of CSM. Public health nurses' understandings of young people's sexting practices were shaped largely by media reports, rather than scientific, disciplinary knowledge. Sexting did not resonate with many public health nurses' own experiences of being a young person and was therefore difficult to understand. All were able to express an opinion about the causes and consequences of sexting and we present these as a \"perceived hierarchy of risk\". All public health nurses acknowledged the importance of their role in dealing with harm reduction associated with sexting among young people, but they need education and support to do this effectively and confidently. Findings can be transferred carefully to many contexts and countries because sexting is a practice among young people that transcends geographical boundaries.", "gemini": "Trong thập kỷ qua, những tác hại tiềm ẩn liên quan đến việc sử dụng công nghệ của những người trẻ tuổi đã thu hút sự chú ý ngày càng tăng của giới chính trị, truyền thông và nghiên cứu trên toàn thế giới. Một hành vi mà nhiều người trẻ tuổi tham gia là \"gửi tin nhắn khiêu dâm\" và chia sẻ hình ảnh khỏa thân một phần hoặc toàn bộ (\"tự sướng\"). Những hình ảnh như vậy không phải lúc nào cũng được giữ trong không gian riêng tư và có khả năng bị chia sẻ, gây ra hậu quả tâm lý xã hội đáng kể cho những người trẻ tuổi liên quan. Một rủi ro đáng kể là bản chất ẩn của một số tương tác trực tuyến, có khả năng dẫn đến việc dụ dỗ và xâm hại tình dục trẻ em. Là những chuyên gia chính làm việc với những người trẻ tuổi, y tá y tế công cộng có khả năng giáo dục và khám phá những rủi ro với họ. Tuy nhiên, cho đến nay, theo hiểu biết của chúng tôi, vẫn chưa có nghiên cứu nào liên quan đến sự hiểu biết của các y tá y tế công cộng về các hành vi liên quan hoặc những tác hại tiềm ẩn của họ. Một nghiên cứu định tính đã được thực hiện dựa trên lý thuyết về mô hình thường thức (CSM) để đóng khung phân tích. Mười tám cuộc phỏng vấn bán cấu trúc đã được thực hiện với các y tá y tế công cộng tại một khu vực của Anh vào năm 2016. Dữ liệu được phân tích thông qua phân tích chủ đề và được ánh xạ vào năm lĩnh vực của CSM. Sự hiểu biết của các y tá y tế công cộng về các hành vi nhắn tin khiêu dâm của những người trẻ tuổi phần lớn được định hình bởi các báo cáo của phương tiện truyền thông, chứ không phải kiến thức khoa học, chuyên ngành. Việc nhắn tin khiêu dâm không phù hợp với kinh nghiệm của nhiều y tá y tế công cộng khi còn là một người trẻ tuổi và do đó rất khó hiểu. Tất cả đều có thể bày tỏ ý kiến về nguyên nhân và hậu quả của việc nhắn tin khiêu dâm và chúng tôi trình bày những ý kiến này như một \"thứ bậc rủi ro được nhận thức\". Tất cả các y tá y tế công cộng đều thừa nhận tầm quan trọng của vai trò của họ trong việc đối phó với việc giảm tác hại liên quan đến việc nhắn tin khiêu dâm ở những người trẻ tuổi, nhưng họ cần được giáo dục và hỗ trợ để thực hiện điều này một cách hiệu quả và tự tin. Các phát hiện có thể được chuyển một cách cẩn thận đến nhiều bối cảnh và quốc gia vì nhắn tin khiêu dâm là một hành vi của những người trẻ tuổi vượt qua ranh giới địa lý."} +{"idx": 1890071, "en": "OBJECTIVE To investigate whether diabetic nephropathy (DN) is associated with lower quality of life (QOL) in youths with long-duration type 1 diabetes and whether associations differ by diabetes duration. METHODS Overall, 1,462 youths aged 11 to 17 years with diabetes onset between 0 and 4 years of age and at least 10 years diabetes duration completed questionnaires on QOL between 2009 and 2016. Chronic generic and diabetes-specific QOL (diabetes impact and treatment scale) were assessed with three scales of the DISABKIDS instruments. Information on DN was obtained from the diabetes patient follow-up registry (\"Diabetes-Patienten-Verlaufsdokumentation\") with DN defined as micro- or macroalbuminuria. Linear regression analyses were used to evaluate the association between QOL and DN. To adjust for potential confounders, we applied inverse probability of treatment weighting for the linear regression. RESULTS In adjusted analysis, DN was associated with lower QOL in the chronic generic and the two diabetes-specific DISABKIDS scales. Overall, the observed effects were not clinically relevant but increased consistently with longer diabetes duration. Among those with at least 16 years diabetes duration, differences in QOL between patients with vs without DN were clinically relevant on the chronic generic scale (β = -10.3 [-21.0;0.7]). CONCLUSION The results suggest that long-term microvascular complications can impair chronic generic QOL already in youths with type 1 diabetes. Differences in QOL between patients with and without DN seem to increase with diabetes duration.", "gemini": "MỤC TIÊU Để điều tra xem bệnh lý thận do đái tháo đường (DN) có liên quan đến chất lượng cuộc sống (QOL) thấp hơn ở những thanh thiếu niên mắc bệnh đái tháo đường type 1 kéo dài hay không và liệu các mối liên quan có khác nhau tùy theo thời gian mắc bệnh tiểu đường hay không. PHƯƠNG PHÁP Nhìn chung, 1.462 thanh thiếu niên từ 11 đến 17 tuổi mắc bệnh tiểu đường khởi phát từ 0 đến 4 tuổi và thời gian mắc bệnh tiểu đường ít nhất 10 năm đã hoàn thành các bảng câu hỏi về QOL từ năm 2009 đến năm 2016. QOL chung mãn tính và QOL đặc hiệu cho bệnh tiểu đường (tác động của bệnh tiểu đường và thang điều trị) đã được đánh giá bằng ba thang đo của các công cụ DISABKIDS. Thông tin về DN được lấy từ sổ đăng ký theo dõi bệnh nhân tiểu đường (\"Diabetes-Patienten-Verlaufsdokumentation\") với DN được định nghĩa là vi niệu hoặc vĩ niệu. Các phân tích hồi quy tuyến tính đã được sử dụng để đánh giá mối liên quan giữa QOL và DN. Để điều chỉnh các yếu tố gây nhiễu tiềm ẩn, chúng tôi đã áp dụng xác suất nghịch đảo của trọng số điều trị cho hồi quy tuyến tính. KẾT QUẢ Trong phân tích điều chỉnh, DN có liên quan đến QOL thấp hơn trong các thang đo DISABKIDS chung mãn tính và hai thang đo đặc hiệu cho bệnh tiểu đường. Nhìn chung, các tác động quan sát được không có liên quan về mặt lâm sàng nhưng tăng đều đặn theo thời gian mắc bệnh tiểu đường dài hơn. Trong số những người mắc bệnh tiểu đường ít nhất 16 năm, sự khác biệt về QOL giữa những bệnh nhân có và không có DN có liên quan về mặt lâm sàng trên thang đo chung mãn tính (β = -10,3 [-21,0; 0,7]). KẾT LUẬN Kết quả cho thấy rằng các biến chứng vi mạch lâu dài có thể làm suy yếu QOL chung mãn tính ngay ở những thanh thiếu niên mắc bệnh tiểu đường type 1. Sự khác biệt về QOL giữa những bệnh nhân có và không có DN dường như tăng lên theo thời gian mắc bệnh tiểu đường."} +{"idx": 1890072, "en": "Increasing numbers of neuroimaging studies are acquiring data to examine changes in brain architecture by investigating intrinsic functional networks (IFN) from longitudinal resting-state functional MRI (rs-fMRI). At the subject level, these IFNs are determined by cross-sectional procedures, which neglect intra-subject dependencies and result in suboptimal estimates of the networks. Here, a novel longitudinal approach simultaneously extracts subject-specific IFNs across multiple visits by explicitly modeling functional brain development as an essential context for seeking change. On data generated by an innovative simulation based on real rs-fMRI, the method was more accurate in estimating subject-specific IFNs than cross-sectional approaches. Furthermore, only group-analysis based on longitudinally consistent estimates identified significant developmental effects within IFNs of 246 adolescents from the National Consortium on Alcohol and NeuroDevelopment in Adolescence (NCANDA) study. The findings were confirmed by the cross-sectional estimates when the corresponding group analysis was confined to the developmental effects. Those effects also converged with current concepts of neurodevelopment.", "gemini": "Ngày càng có nhiều nghiên cứu về ảnh thần kinh đang thu thập dữ liệu để kiểm tra những thay đổi trong cấu trúc não bằng cách nghiên cứu các mạng chức năng nội tại (IFN) từ chụp cộng hưởng từ chức năng trạng thái nghỉ dọc theo thời gian (rs-fMRI). Ở cấp độ đối tượng, các IFN này được xác định bằng các quy trình cắt ngang, bỏ qua sự phụ thuộc giữa các đối tượng và dẫn đến ước tính không tối ưu của các mạng. Ở đây, một phương pháp tiếp cận theo chiều dọc mới đồng thời trích xuất các IFN cụ thể của đối tượng trong nhiều lần khám bằng cách mô hình hóa rõ ràng sự phát triển chức năng của não như một bối cảnh thiết yếu để tìm kiếm sự thay đổi. Trên dữ liệu được tạo ra bởi một mô phỏng sáng tạo dựa trên rs-fMRI thực, phương pháp này chính xác hơn trong việc ước tính các IFN cụ thể của đối tượng so với các phương pháp cắt ngang. Hơn nữa, chỉ phân tích nhóm dựa trên các ước tính nhất quán theo chiều dọc mới xác định được các hiệu ứng phát triển đáng kể trong các IFN của 246 thanh thiếu niên từ Nghiên cứu của Liên đoàn quốc gia về rượu và phát triển thần kinh ở tuổi vị thành niên (NCANDA). Những phát hiện đã được xác nhận bằng các ước tính cắt ngang khi phân tích nhóm tương ứng được giới hạn ở các hiệu ứng phát triển. Những hiệu ứng đó cũng hội tụ với các khái niệm hiện tại về sự phát triển thần kinh."} +{"idx": 1890073, "en": "Layered lithium-rich cathode materials are one of the most promising cathode materials owing to their higher mass energy density than the commercial counterparts. A series of trace Yb-doped lithium-rich cathode materials Li1.2 Mn0.54 Ni0.13 Co0.13-x Ybx O2 (0≤x≤0.050) were synthesized and the effects were investigated by XRD, X-ray photoelectron spectroscopy, and high-resolution TEM. The participation of Yb ions in electrochemical reactions and the larger binding energy of Yb-O than M-O (M=Mn, Ni, Co), which expands the lithium layer spacing and stabilizes the oxygen stacking, resulted in excellent performance of materials doped with a limited Yb content (x≤0.005). However, higher doping amounts (x>0.005) significantly increased the charge-transfer impedance and led to a sharp deterioration in electrochemical performance. The reason lies in the large difference in ionic radius between the transition metals (Mn, Co, and Ni) and Yb. There is an upper limit to the amount of Yb ions in the lattice. If the amount of Yb is higher than the limit, excess Yb ions enter the Li layers instead of staying in the transition-metal layers or even segregate on the surface and form electrochemically inert oxides.", "gemini": "Vật liệu catốt giàu lithium nhiều lớp là một trong những vật liệu catốt hứa hẹn nhất do mật độ năng lượng khối cao hơn so với các vật liệu thương mại. Một loạt các vật liệu catốt giàu lithium có vết Yb Li1.2 Mn0.54 Ni0.13 Co0.13-x Ybx O2 (0≤x≤0.050) đã được tổng hợp và các hiệu ứng đã được nghiên cứu bằng XRD, quang phổ quang điện tử tia X và TEM độ phân giải cao. Sự tham gia của các ion Yb trong các phản ứng điện hóa và năng lượng liên kết lớn hơn của Yb-O so với M-O (M = Mn, Ni, Co), làm giãn khoảng cách lớp lithium và ổn định sự xếp chồng của oxy, dẫn đến hiệu suất tuyệt vời của các vật liệu được trộn với hàm lượng Yb giới hạn (x≤0.005). Tuy nhiên, lượng tạp chất cao hơn (x> 0,005) làm tăng đáng kể trở kháng truyền điện tích và dẫn đến hiệu suất điện hóa giảm mạnh. Nguyên nhân nằm ở sự chênh lệch lớn về bán kính ion giữa các kim loại chuyển tiếp (Mn, Co và Ni) và Yb. Có một giới hạn trên về lượng ion Yb trong mạng tinh thể. Nếu lượng Yb cao hơn giới hạn, các ion Yb dư sẽ đi vào các lớp Li thay vì ở lại trong các lớp kim loại chuyển tiếp hoặc thậm chí tách ra trên bề mặt và tạo thành các oxit trơ về mặt điện hóa."} +{"idx": 1890074, "en": "Several reports have shown that methyl CpG-binding protein 2 (MeCP2), brain-derived neurotrophic factor (BDNF), phospho-cAMP response element-binding protein (p-CREB) and microRNAs may be important in regulating academic performance because of their roles in neuropsychiatry and cognitive diseases. The first goal of this study was to explore the associations among MeCP2, BDNF, CREB and academic performance. This study also examined the pathway responsible for the effects of MeCP2, BDNF, p-CREB and microRNAs on academic performance. Scores from the basic competency test, an annual national competitive entrance examination, were used to evaluate academic performance. Subjects' plasma RNA was extracted and analyzed. This study determined that participants in the higher academic performance group had a significant difference in MECP2 mRNA expression compared with the lower academic performance group. We then used neuronal human derived neuroblastoma cell line (SH-SY5Y) cells with inducible MeCP2 expression from a second copy of the gene as a gain-of-function model and found that MeCP2 overexpression positively affected p-CREB and BDNF expression initially. After negative feedback, the p-CREB and BDNF levels subsequently decreased. In the neuronal phenotype examination, we found a significant reduction in total outgrowth and branches in MeCP2-induced cells compared with noninduced cells. This work describes pathways that may be responsible for the effects of MeCP2, BDNF, p-CREB and microRNAs on academic performance. These results may shed light on the development of promising clinical treatment strategies in the area of neuropsychological adjustment.", "gemini": "Một số báo cáo đã chỉ ra rằng protein liên kết methyl CpG-2 (MeCP2), yếu tố dinh dưỡng thần kinh có nguồn gốc từ não (BDNF), protein liên kết yếu tố đáp ứng phospho-cAMP (p-CREB) và microRNA có thể đóng vai trò quan trọng trong việc điều chỉnh thành tích học tập vì vai trò của chúng trong thần kinh học và các bệnh về nhận thức. Mục tiêu đầu tiên của nghiên cứu này là khám phá mối liên quan giữa MeCP2, BDNF, CREB và thành tích học tập. Nghiên cứu này cũng kiểm tra con đường chịu trách nhiệm cho các tác động của MeCP2, BDNF, p-CREB và microRNA đối với thành tích học tập. Điểm số từ bài kiểm tra năng lực cơ bản, một kỳ thi tuyển sinh cạnh tranh toàn quốc hàng năm, được sử dụng để đánh giá thành tích học tập. RNA huyết tương của đối tượng được chiết xuất và phân tích. Nghiên cứu này xác định rằng những người tham gia trong nhóm có thành tích học tập cao hơn có sự khác biệt đáng kể về biểu hiện mRNA MECP2 so với nhóm có thành tích học tập thấp hơn. Sau đó, chúng tôi đã sử dụng các tế bào dòng tế bào thần kinh đệm thần kinh nguyên bào nuôi cấy của con người (SH-SY5Y) với biểu hiện MeCP2 có thể gây ra từ bản sao thứ hai của gen làm mô hình tăng chức năng và phát hiện ra rằng sự biểu hiện quá mức của MeCP2 ảnh hưởng tích cực đến biểu hiện p-CREB và BDNF ban đầu. Sau phản hồi tiêu cực, mức p-CREB và BDNF sau đó giảm. Trong quá trình kiểm tra kiểu hình nơ-ron, chúng tôi phát hiện thấy sự giảm đáng kể về tổng số nhánh và nhánh ở các tế bào do MeCP2 gây ra so với các tế bào không gây ra. Nghiên cứu này mô tả các con đường có thể chịu trách nhiệm cho các tác động của MeCP2, BDNF, p-CREB và microRNA đối với thành tích học tập. Những kết quả này có thể làm sáng tỏ sự phát triển của các chiến lược điều trị lâm sàng đầy hứa hẹn trong lĩnh vực điều chỉnh tâm lý thần kinh."} +{"idx": 1890075, "en": "In smokers, neural responses to smoking cues can be sensitive to acute abstinence, but the degree to which abstinence-related cue reactivity contributes to relapse is not fully understood. This study addressed this question in a sample of 75 smokers who were motivated to quit smoking. Participants underwent blood-oxygen-level-dependent (BOLD) functional magnetic resonance imaging (fMRI) during presentation of visual smoking cues and neutral stimuli on two occasions: once during smoking satiety and once following 24-hour abstinence (order counterbalanced). Following the imaging sessions, participants received brief smoking cessation counseling prior to a short-term (7-day) quit attempt. The primary smoking cessation outcome was biochemically confirmed 7-day relapse. The secondary smoking cessation outcome measure was total number of self-reported days of abstinence. During abstinence (vs satiety), smoking cue reactivity was significantly increased only in the anterior cingulate cortex (ACC); other regions showing a cue (vs neutral) response did not exhibit an abstinence effect in the stringent whole-brain analysis. Participants who showed greater smoking cue reactivity in the ACC during acute abstinence (compared with smoking satiety) were more likely to relapse (OR = 2.10 per standard deviation increase in percent signal change [abstinence minus smoking satiety], 95% CI: 1.05 to 4.20, P = 0.036). Greater abstinence-induced change in ACC activation also predicted fewer total days abstinent (β = -0.63, 95% CI = 0.43 to 0.66, P < 0.0001). This study provides the first evidence that changes in smoking cue reactivity in the ACC during acute abstinence predict smoking relapse, thereby improving our understanding of the neurobiology of smoking cessation.", "gemini": "Ở những người hút thuốc, phản ứng thần kinh với tín hiệu hút thuốc có thể nhạy cảm với việc cai thuốc cấp tính, nhưng mức độ mà phản ứng tín hiệu liên quan đến việc cai thuốc góp phần vào việc tái nghiện vẫn chưa được hiểu đầy đủ. Nghiên cứu này giải quyết câu hỏi này trong một mẫu gồm 75 người hút thuốc có động lực bỏ thuốc lá. Những người tham gia đã trải qua chụp cộng hưởng từ chức năng phụ thuộc mức oxy trong máu (BOLD) trong khi trình bày các tín hiệu hút thuốc trực quan và các kích thích trung tính vào hai lần: một lần trong thời gian thỏa mãn hút thuốc và một lần sau khi cai thuốc trong 24 giờ (thứ tự được cân bằng). Sau các buổi chụp hình, những người tham gia được tư vấn cai thuốc lá ngắn gọn trước khi thử cai thuốc trong thời gian ngắn (7 ngày). Kết quả cai thuốc lá chính được xác nhận bằng sinh hóa là tái nghiện trong 7 ngày. Biện pháp kết quả cai thuốc lá thứ cấp là tổng số ngày cai thuốc tự báo cáo. Trong thời gian cai thuốc (so với thời gian thỏa mãn), phản ứng tín hiệu hút thuốc chỉ tăng đáng kể ở vỏ não vành trước (ACC); các vùng khác cho thấy phản ứng tín hiệu (so với trung tính) không biểu hiện tác dụng cai thuốc trong phân tích toàn bộ não nghiêm ngặt. Những người tham gia có phản ứng tín hiệu hút thuốc lớn hơn ở ACC trong thời gian cai thuốc cấp tính (so với thời gian thỏa mãn hút thuốc) có khả năng tái nghiện cao hơn (OR = 2,10 trên mỗi độ lệch chuẩn tăng phần trăm thay đổi tín hiệu [cai thuốc trừ thỏa mãn hút thuốc], KTC 95%: 1,05 đến 4,20, P = 0,036). Thay đổi do cai thuốc gây ra lớn hơn trong kích hoạt ACC cũng dự đoán ít ngày cai thuốc hơn (β = -0,63, KTC 95% = 0,43 đến 0,66, P < 0,0001). Nghiên cứu này cung cấp bằng chứng đầu tiên cho thấy những thay đổi trong phản ứng tín hiệu hút thuốc ở ACC trong thời gian cai thuốc cấp tính dự đoán tái nghiện hút thuốc, do đó cải thiện sự hiểu biết của chúng ta về sinh học thần kinh của việc cai thuốc lá."} +{"idx": 1890076, "en": "Development of low-cost water-/alcohol-soluble interfacial materials is a crucial issue to promote the commercialization of polymer solar cells (PSCs). Herein, two derivatives of low-cost rhodamine, called sulforhodamine 101 (SR101) and sulforhodamine B (SRB), are applied as cathode interfacial layers (CILs) to effectively improve the charge-carrier transportation and collection, reduce the work function (WF) of Al counter electrode, and decrease the series resistance and charge recombination in the PSCs. As a result, SR101-based devices show excellent performance with the highest power conversion efficiency (PCE) of 9.10 %, superior to that of both the control devices with MeOH/Al and Ca/Al. Notably, sulforhodamine is commercially available with low cost and great solution-processability. This work demonstrates that sulforhodamine has a great potential as a CIL material,which is suitable for the large-area fabrication process and commercialization of highly efficient PSCs.", "gemini": "Phát triển các vật liệu giao diện hòa tan trong nước/rượu có giá thành thấp là một vấn đề quan trọng để thúc đẩy thương mại hóa các tế bào quang điện polyme (PSC). Ở đây, hai dẫn xuất của rhodamine giá rẻ, được gọi là sulforhodamine 101 (SR101) và sulforhodamine B (SRB), được ứng dụng làm lớp giao diện catot (CIL) để cải thiện hiệu quả vận chuyển và thu thập điện tích, giảm hàm công (WF) của điện cực đối Al, và giảm điện trở nối tiếp và tái tổ hợp điện tích trong PSC. Kết quả là, các thiết bị dựa trên SR101 cho thấy hiệu suất tuyệt vời với hiệu suất chuyển đổi công suất (PCE) cao nhất là 9,10%, vượt trội so với cả các thiết bị đối chứng với MeOH/Al và Ca/Al. Đáng chú ý, sulforhodamine được bán trên thị trường với giá thành thấp và khả năng xử lý dung dịch tuyệt vời. Công trình này chứng minh rằng sulforhodamine có tiềm năng lớn như một vật liệu CIL, phù hợp với quy trình chế tạo diện tích lớn và thương mại hóa các PSC có hiệu suất cao."} +{"idx": 1890077, "en": "OBJECTIVE To investigate the volumetric and functional connectivity of the habenular nucleus in treatment-resistant depression (TRD) patients using the resting-state functional magnetic resonance imaging (rs-fMRI) approach. METHODS A total of 15 TRD patients, who visited the Mental Health Institute of the First Hospital Affiliated with Jilin University between August 2014 and March 2015, along with 15 normal subjects, were enrolled into this study for structural and functional imaging. Functional connectivity analysis was performed using bilateral habenular nuclei as the region of interest in contrast to whole-brain voxels. RESULTS No significant difference of absolute volume was found in bilateral habenular nuclei between TRD patients and healthy controls, or after controlling for individual total intracranial volume. However, functional connectivity analysis showed increased connectivity between the right habenular nucleus with the medial superior frontal gyrus, anterior cingulate cortex and medial orbitofrontal gyrus, and decreased connectivity with the corpus callosum in the TRD group. For the left habenular nucleus seed, the brain region with increased functional connectivity in the inferior temporal gyrus and decreased functional connectivity in the insular was found in the TRD patients. CONCLUSION Abnormal functional connectivity was present between the habenular nucleus and the default mode network in TRD patients. Dysfunction in habenular nucleus-related circuitry for processing negative emotion might form the pathological basis for TRD. Significant asymmetric functional connectivity was also found between bilateral habenular nuclei in TRD patients. Such asymmetry suggests potentially divergent strategy for intervention on bilateral habenular nucleus regions in the future management of depression.", "gemini": "MỤC TIÊU Nghiên cứu kết nối thể tích và chức năng của nhân não thất trong bệnh trầm cảm kháng trị (TRD) bằng phương pháp chụp cộng hưởng từ chức năng trạng thái nghỉ (rs-fMRI). PHƯƠNG PHÁP Tổng cộng 15 bệnh nhân TRD đến khám tại Viện Sức khỏe Tâm thần của Bệnh viện số 1 trực thuộc Đại học Cát Lâm từ tháng 8 năm 2014 đến tháng 3 năm 2015, cùng với 15 đối tượng bình thường, đã được tuyển dụng vào nghiên cứu này để chụp ảnh cấu trúc và chức năng. Phân tích kết nối chức năng được thực hiện bằng cách sử dụng nhân não thất hai bên làm vùng quan tâm trái ngược với các voxel toàn não. KẾT QUẢ Không tìm thấy sự khác biệt đáng kể về thể tích tuyệt đối ở nhân não thất hai bên giữa bệnh nhân TRD và đối chứng khỏe mạnh, hoặc sau khi kiểm soát thể tích nội sọ toàn bộ của từng cá nhân. Tuy nhiên, phân tích kết nối chức năng cho thấy kết nối tăng lên giữa nhân não thất phải với hồi trán trên giữa, vỏ não vành trước và hồi quỹ đạo giữa, và kết nối giảm với thể chai trong nhóm TRD. Đối với hạt giống nhân não thất trái, vùng não có kết nối chức năng tăng lên ở hồi thái dương dưới và kết nối chức năng giảm ở đảo được tìm thấy ở bệnh nhân TRD. KẾT LUẬN Kết nối chức năng bất thường có mặt giữa nhân não thất và mạng chế độ mặc định ở bệnh nhân TRD. Rối loạn chức năng trong mạch liên quan đến nhân não thất để xử lý cảm xúc tiêu cực có thể hình thành cơ sở bệnh lý cho TRD. Kết nối chức năng không đối xứng đáng kể cũng được tìm thấy giữa nhân não thất hai bên ở bệnh nhân TRD. Sự không đối xứng như vậy gợi ý chiến lược can thiệp khác biệt tiềm ẩn trên các vùng nhân não thất hai bên trong việc quản lý bệnh trầm cảm trong tương lai."} +{"idx": 1890078, "en": "OBJECTIVE To compare methods of assessment of the burden of primary care-type ED (PCTED) presentations against clinical assessment by general practitioners (GPs) in ED. METHODS A cross-sectional study involving clinical assessment of patients presenting to four EDs in Western Australia. The GPs assessed patients who were likely to be discharged home from ED, and considered whether they could be managed in general practice. Patient presentations were defined by the GPs as: PCTED; PCTED if additional primary care resources were available; or not PCTED. RESULTS GP researchers determined that 80% of patients assessed were PCTED presentations, with one-third of these considered PCTED presentations if additional resources were available. A high proportion of identified PCTED presentations included categories excluded by previous methods. Analysis of linked data found the cohort assessed to be of lower urgency, younger, and with a shorter length of stay than the average patient being discharged from ED. After accounting for potential bias, it is suggested that 20-40% of all ED presentations could be PCTED presentations. CONCLUSIONS Previous methods determining the burden of PCTED presentations have not been validated. Many presentations excluded by previous methods were identified as manageable in general practice by GPs clinically assessing patients in ED. Improved validation of criteria used to identify PCTED presentations will enable appropriately designed interventions to reduce such events.", "gemini": "MỤC TIÊU So sánh các phương pháp đánh giá gánh nặng của các lần khám bệnh cấp cứu loại chăm sóc ban đầu (PCTED) so với đánh giá lâm sàng của các bác sĩ đa khoa (GP) trong khoa cấp cứu. PHƯƠNG PHÁP Một nghiên cứu cắt ngang liên quan đến đánh giá lâm sàng của những bệnh nhân đến bốn khoa cấp cứu ở Tây Úc. Các bác sĩ đa khoa đã đánh giá những bệnh nhân có khả năng được xuất viện về nhà từ khoa cấp cứu và xem xét liệu họ có thể được quản lý trong phòng khám đa khoa hay không. Các bác sĩ đa khoa định nghĩa các lần khám bệnh của bệnh nhân là: PCTED; PCTED nếu có thêm các nguồn chăm sóc ban đầu; hoặc không phải PCTED. KẾT QUẢ Các nhà nghiên cứu bác sĩ đa khoa xác định rằng 80% bệnh nhân được đánh giá là các lần khám bệnh PCTED, với một phần ba trong số này được coi là các lần khám bệnh PCTED nếu có thêm các nguồn lực. Tỷ lệ cao các lần khám bệnh PCTED được xác định bao gồm các loại bị loại trừ bởi các phương pháp trước đây. Phân tích dữ liệu liên kết cho thấy nhóm bệnh nhân được đánh giá có mức độ cấp cứu thấp hơn, trẻ hơn và thời gian lưu trú ngắn hơn so với bệnh nhân trung bình được xuất viện khỏi khoa cấp cứu. Sau khi tính đến khả năng thiên vị, người ta cho rằng 20-40% trong số tất cả các lần khám bệnh cấp cứu có thể là các lần khám bệnh PCTED. KẾT LUẬN Các phương pháp trước đây xác định gánh nặng của các lần khám bệnh PCTED vẫn chưa được xác nhận. Nhiều lần khám bệnh bị loại trừ bởi các phương pháp trước đây đã được các bác sĩ đa khoa đánh giá lâm sàng bệnh nhân trong khoa cấp cứu xác định là có thể quản lý được trong phòng khám đa khoa. Việc xác nhận được cải thiện các tiêu chí được sử dụng để xác định các lần khám bệnh PCTED sẽ cho phép thiết kế các biện pháp can thiệp phù hợp để giảm các sự kiện như vậy."} +{"idx": 1890079, "en": "BACKGROUND To evaluate whether pre-emptive skin analgesia using a lidocaine patch 5% would improve the effects of systemic morphine analgesia for controlling acute post-thoracotomy pain. METHODS This was a double-blind, placebo controlled, prospective study. Patients were randomly assigned to receive lidocaine 5% patch (lidocaine group) or a placebo (placebo group) three days before thoracotomy. Postoperative analgesia was induced in all cases with intravenous morphine analgesia. The intergroup differences were assessed in order to evaluate whether the lidocaine patch 5% would have effects on pain intensity when at rest and after coughing (primary end-point) on morphine consumption, on the recovery of respiratory function, and on peripheral painful pathways measured with N2 and P2 laser-evoked potential (secondary end-points). RESULTS A total of 90 patients were randomized, of whom 45 were allocated to the lidocaine group and 45 to the placebo group. Lidocaine compared with the placebo group showed a significant reduction in pain intensity both at rest (P = 0.013) and after coughing (P = 0.015), and in total morphine consumption (P = 0.001); and also showed a better recovery of flow expiratory volume in one second (P = 0.025) and of forced vital capacity (P = 0.037). The placebo group compared with the lidocaine group presented a reduction in amplitude of N2 (P = 0.001) and P2 (P = 0.03), and an increase in the latency of N2 (P = 0.023) and P2 (P = 0.025) laser-evoked potential. CONCLUSIONS The preventive skin analgesia with lidocaine patch 5% seems to be a valid adjunct to intravenous morphine analgesia for controlling post-thoracotomy pain. However, our initial results should be corroborated/confirmed by larger studies.", "gemini": "BỐI CẢNH Đánh giá xem liệu việc giảm đau da trước bằng miếng dán lidocaine 5% có cải thiện tác dụng của thuốc giảm đau morphine toàn thân để kiểm soát cơn đau cấp tính sau phẫu thuật cắt bỏ thùy phổi hay không. PHƯƠNG PHÁP Đây là một nghiên cứu có đối chứng giả dược, mù đôi, có triển vọng. Bệnh nhân được chỉ định ngẫu nhiên nhận miếng dán lidocaine 5% (nhóm lidocaine) hoặc giả dược (nhóm giả dược) ba ngày trước khi cắt bỏ thùy phổi. Giảm đau sau phẫu thuật được gây ra trong tất cả các trường hợp bằng thuốc giảm đau morphine tiêm tĩnh mạch. Các khác biệt giữa các nhóm được đánh giá để đánh giá xem miếng dán lidocaine 5% có ảnh hưởng đến cường độ đau khi nghỉ ngơi và sau khi ho (điểm cuối chính) khi dùng morphine, phục hồi chức năng hô hấp và các đường đau ngoại biên được đo bằng tiềm năng gây ra bằng laser N2 và P2 (điểm cuối thứ cấp). KẾT QUẢ Tổng cộng 90 bệnh nhân được phân ngẫu nhiên, trong đó 45 bệnh nhân được phân vào nhóm lidocaine và 45 bệnh nhân được phân vào nhóm giả dược. Lidocaine so với nhóm giả dược cho thấy giảm đáng kể cường độ đau khi nghỉ ngơi (P = 0,013) và sau khi ho (P = 0,015) và tổng lượng morphine tiêu thụ (P = 0,001); và cũng cho thấy phục hồi tốt hơn thể tích thở ra trong một giây (P = 0,025) và sức sống phổi (P = 0,037). Nhóm giả dược so với nhóm lidocaine cho thấy giảm biên độ N2 (P = 0,001) và P2 (P = 0,03) và tăng thời gian tiềm ẩn của tiềm năng gây ra bằng laser N2 (P = 0,023) và P2 (P = 0,025). KẾT LUẬN Giảm đau da dự phòng bằng miếng dán lidocaine 5% có vẻ là một phương pháp bổ sung hợp lệ cho thuốc giảm đau morphine tiêm tĩnh mạch để kiểm soát cơn đau sau cắt bỏ thùy phổi. Tuy nhiên, kết quả ban đầu của chúng tôi nên được xác nhận/chứng minh bằng các nghiên cứu lớn hơn."} +{"idx": 1890080, "en": "The contribution of Adenosine (ADO) to exercise hyperemia remains controversial and it is unknown whether ADO can evoke the prolonged vasodilation seen during exercise bouts. Therefore, we tested hypotheses in the human forearm during 3 h of intra-arterial high dose ADO infusion: (1) skeletal muscle blood flow would wane over time; (2) exercise hyperemic responses during ADO administration would be unaffected compared to baseline. Using sodium nitroprusside (SNP), we tested parallel hypotheses regarding nitric oxide (NO) in a separate group of participants. Seventeen young healthy participants (ADO: n = 9; SNP: n = 8) performed multiple rhythmic handgrip exercise bouts (20% of maximum), two during saline and five during 3 h of continuous drug infusion. Five minutes of ADO infusion resulted in a ~5-fold increase in forearm vascular conductance (FVC; 4.8 ± 0.6 vs. 24.2 ± 3.2 mL/min/100 mmHg, P < 0.05). SNP caused a ~4-fold increase (4.4 ± 0.6 vs. 16.6 ± 2 mL/min/100 mmHg, P < 0.05). FVC did not wane over time with ADO (24.2 ± 3.2 and 22 ± 1.2 mL/min/100 mmHg [P > 0.05]) or SNP (16.6 ± 2 and 14.1 ± 2.4 mL/min/ 100 mmHg [P > 0.05]) at 5 versus 150 min. Superimposed exercise during ADO or SNP infusions evoked marked and consistent additional dilation over the course of the infusions. Our findings demonstrate that in humans there is no reduction in endothelial or vascular smooth muscle responsiveness to the exogenous vasodilatory metabolites ADO and NO. Additionally, even in the presence of an exogenous vasodilator, superimposed exercise can cause significant hyperemia.", "gemini": "Đóng góp của Adenosine (ADO) vào chứng tăng huyết áp khi tập thể dục vẫn còn gây tranh cãi và không biết liệu ADO có thể gây ra chứng giãn mạch kéo dài khi tập thể dục hay không. Do đó, chúng tôi đã kiểm tra các giả thuyết ở cẳng tay của người trong quá trình truyền ADO liều cao trong động mạch trong 3 giờ: (1) lưu lượng máu cơ xương sẽ giảm dần theo thời gian; (2) phản ứng tăng huyết áp khi tập thể dục trong quá trình dùng ADO sẽ không bị ảnh hưởng so với mức cơ bản. Sử dụng natri nitroprusside (SNP), chúng tôi đã kiểm tra các giả thuyết song song liên quan đến oxit nitric (NO) trong một nhóm người tham gia riêng biệt. Mười bảy người tham gia khỏe mạnh trẻ tuổi (ADO: n = 9; SNP: n = 8) đã thực hiện nhiều đợt tập luyện cầm tay nhịp nhàng (20% tối đa), hai lần trong nước muối và năm lần trong quá trình truyền thuốc liên tục trong 3 giờ. Năm phút truyền ADO dẫn đến tăng khoảng 5 lần về độ dẫn mạch ở cẳng tay (FVC; 4,8 ± 0,6 so với 24,2 ± 3,2 mL/phút/100 mmHg, P < 0,05). SNP gây ra sự gia tăng khoảng 4 lần (4,4 ± 0,6 so với 16,6 ± 2 mL/phút/100 mmHg, P < 0,05). FVC không giảm theo thời gian với ADO (24,2 ± 3,2 và 22 ± 1,2 mL/phút/100 mmHg [P> 0,05]) hoặc SNP (16,6 ± 2 và 14,1 ± 2,4 mL/phút/100 mmHg [P> 0,05]) ở phút thứ 5 so với phút thứ 150. Tập thể dục chồng chéo trong quá trình truyền ADO hoặc SNP gây ra sự giãn nở bổ sung rõ rệt và nhất quán trong quá trình truyền. Những phát hiện của chúng tôi chứng minh rằng ở người, không có sự giảm đáp ứng của nội mô hoặc cơ trơn mạch máu đối với các chất chuyển hóa giãn mạch ngoại sinh ADO và NO. Ngoài ra, ngay cả khi có chất giãn mạch ngoại sinh, tập thể dục chồng chéo vẫn có thể gây ra chứng tăng huyết áp đáng kể."} +{"idx": 1890081, "en": "OBJECTIVE To identify Rehabilitation Indices associated with a minimal trauma fracture (MTF) within 12 months poststroke. METHODS Retrospective case-control study. Stroke survivors with MTF were matched 5:1 with stroke survivors without MTF. Logistic regression determined whether Rehabilitation Indices, such as Physiotherapy Ambulation score (PhysioAmb), were associated with a MTF within 12 months poststroke. RESULTS Forty-three stroke survivors (mean age: 79.8; 55.81% female) experienced a MTF (median time to MTF of 1.79 years [IQR 0.70, 4.48]). Those with a MTF within 12 months had lower PhysioAmb (4.53 vs 8.29) and Berg Balance Scale (BBS; 12.25 vs 40.57) scores on admission, lower BBS score on discharge (30.33 vs 49.29) and a greater change in PhysioAmb (+5.67 vs +3.24) and BBS scores (+21.50 vs +8.71) after rehabilitation, all P < 0.05. Greater changes in PhysioAmb score increased the odds of a MTF within 12 months by 18%. CONCLUSION Rehabilitation Indices are associated with a MTF within 12 months poststroke.", "gemini": "MỤC TIÊU Nhận dạng các Chỉ số Phục hồi chức năng có liên quan đến gãy xương chấn thương tối thiểu (MTF) trong vòng 12 tháng sau đột quỵ. PHƯƠNG PHÁP Nghiên cứu đối chứng có đối chứng hồi cứu. Những người sống sót sau đột quỵ có MTF được ghép 5:1 với những người sống sót sau đột quỵ không có MTF. Phân tích hồi quy logistic xác định xem các Chỉ số Phục hồi chức năng, chẳng hạn như điểm Đi lại Vật lý trị liệu (PhysioAmb), có liên quan đến MTF trong vòng 12 tháng sau đột quỵ hay không. KẾT QUẢ Bốn mươi ba người sống sót sau đột quỵ (tuổi trung bình: 79,8; 55,81% là nữ) đã trải qua MTF (thời gian trung bình đến MTF là 1,79 năm [IQR 0,70, 4,48]). Những người có MTF trong vòng 12 tháng có điểm PhysioAmb (4,53 so với 8,29) và Điểm Cân bằng Berg (BBS; 12,25 so với 40,57) thấp hơn khi nhập viện, điểm BBS thấp hơn khi xuất viện (30,33 so với 49,29) và thay đổi lớn hơn về điểm PhysioAmb (+5,67 so với +3,24) và điểm BBS (+21,50 so với +8,71) sau khi phục hồi chức năng, tất cả P < 0,05. Những thay đổi lớn hơn về điểm PhysioAmb làm tăng khả năng xảy ra MTF trong vòng 12 tháng lên 18%. KẾT LUẬN Các Chỉ số Phục hồi chức năng có liên quan đến MTF trong vòng 12 tháng sau đột quỵ."} +{"idx": 1890082, "en": "Oxalate decarboxylase (OxDC) from Bacillus subtilis is a Mn-dependent hexameric enzyme that converts oxalate to carbon dioxide and formate. OxDC has greatly attracted the interest of the scientific community, mainly due to its biotechnological and medical applications in particular for the treatment of hyperoxaluria, a group of pathologic conditions caused by oxalate accumulation. The enzyme has an acidic optimum pH, but most of its applications involve processes occurring at neutral pH. Nevertheless, a detailed biochemical characterization of the enzyme at neutral pH is lacking. Here, we compared the structural-functional properties at acidic and neutral pH of wild-type OxDC and of a mutant form, called OxDC-DSSN, bearing four amino acid substitutions in the lid (Ser161-to-Asp, Glu162-to-Ser, Asn163-toSer, and Ser164-to-Asn) that improve the oxalate oxidase activity and almost abolish the decarboxylase activity. We found that both enzymatic forms do not undergo major structural changes as a function of pH, although OxDC-DSSN displays an increased tendency to aggregation, which is counteracted by the presence of an active-site ligand. Notably, OxDC and OxDC-DSSN at pH 7.2 retain 7 and 15% activity, respectively, which is sufficient to degrade oxalate in a cellular model of primary hyperoxaluria type I, a rare inherited disease caused by excessive endogenous oxalate production. The significance of the data in the light of the possible use of OxDC as biological drug is discussed. © 2019 IUBMB Life, 1-11, 2019.", "gemini": "Oxalate decarboxylase (OxDC) từ Bacillus subtilis là một enzyme dạng hexamer phụ thuộc Mn chuyển đổi oxalate thành carbon dioxide và format. OxDC đã thu hút sự quan tâm rất lớn của cộng đồng khoa học, chủ yếu là do các ứng dụng công nghệ sinh học và y tế của nó, đặc biệt là để điều trị tăng oxalat niệu, một nhóm các tình trạng bệnh lý do tích tụ oxalate. Enzyme có độ pH tối ưu là axit, nhưng hầu hết các ứng dụng của nó liên quan đến các quá trình xảy ra ở độ pH trung tính. Tuy nhiên, vẫn chưa có đặc điểm sinh hóa chi tiết của enzyme ở độ pH trung tính. Ở đây, chúng tôi so sánh các đặc tính cấu trúc-chức năng ở độ pH axit và trung tính của OxDC dạng hoang dã và dạng đột biến, được gọi là OxDC-DSSN, mang bốn thế amino axit ở nắp (Ser161 thành Asp, Glu162 thành Ser, Asn163 thành Ser và Ser164 thành Asn) giúp cải thiện hoạt tính oxidase oxalate và gần như xóa bỏ hoạt tính decarboxylase. Chúng tôi thấy rằng cả hai dạng enzyme đều không trải qua những thay đổi cấu trúc lớn theo chức năng pH, mặc dù OxDC-DSSN biểu hiện xu hướng kết tụ tăng lên, điều này được chống lại bởi sự hiện diện của một phối tử tại vị trí hoạt động. Đáng chú ý, OxDC và OxDC-DSSN ở pH 7,2 vẫn giữ lại lần lượt 7 và 15% hoạt tính, đủ để phân hủy oxalate trong mô hình tế bào tăng oxalat niệu nguyên phát type I, một bệnh di truyền hiếm gặp do sản xuất oxalate nội sinh quá mức. Ý nghĩa của dữ liệu dưới góc độ khả năng sử dụng OxDC làm thuốc sinh học được thảo luận. © 2019 IUBMB Life, 1-11, 2019."} +{"idx": 1890083, "en": "Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi-quantitative RT-PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota-1/2016 had a 95.5%-96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota-1/2016 shared an 87.3%-92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%-83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co-infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States.", "gemini": "Pegivirus ở lợn (PPgV) và Pestivirus không điển hình ở lợn (APPV) là hai loại vi-rút ở lợn mới được xác định gần đây. Trong nghiên cứu này, việc xác định hai loại vi-rút bằng giải trình tự siêu gen và một RT-PCR bán định lượng kép đã được phát triển để phát hiện đồng thời các tác nhân gây bệnh này. Chủng PPgV Minnesota-1/2016 có 95,5%-96,3% bản sắc nucleotide và nhóm với các chủng PPgV của Hoa Kỳ mới được xác định gần đây, đây là nhánh xa so với các chủng PPgV của Đức. Chủng APPV Minnesota-1/2016 có 87,3%-92,0% bản sắc nucleotide với các chủng APPV toàn cầu khác nhưng chỉ có 82,8%-83,0% bản sắc nucleotide với nhánh II gồm chủng được xác định ở Trung Quốc. Tỷ lệ phát hiện cả PPgV và APPV là 9,0% các trường hợp chẩn đoán. Nhiễm đồng thời PPgV và APPV được xác định ở 7,5% các trường hợp chẩn đoán. Sự xuất hiện và đặc điểm di truyền của PPgV và APPV giúp nâng cao kiến thức của chúng ta về các tác nhân gây bệnh mới này ở Hoa Kỳ."} +{"idx": 1890084, "en": "BACKGROUND Abnormalities involving chromosome 7 are one of the most frequent chromosomal aberrations in myeloid neoplasms including myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) and are associated with an adverse prognosis. Immunophenotyping by flow cytometry provides data that can assist in the diagnosis and classification of myeloid neoplasms. The immunophenotypic features of myeloid neoplasms with monosomy 7 or del(7q) have not been previously described in a comprehensive fashion. METHODS We retrospectively analyzed flow cytometric data of myeloid neoplasms with monosomy 7 or del(7q) and summarized associated immunophenotypic features. RESULTS Myeloid neoplasms with monosomy 7 typically demonstrate multiple immunophenotypic abnormalities on myeloid blasts and maturing myelomonocytic cells. Increased CD14 expression on maturing granulocytic cells was characteristically seen in myeloid neoplasms with monosomy 7. This abnormality was significantly more frequent in myeloid neoplasms with monosomy 7 than in those with del(7q) (92.9% vs. 8.2%, P < 0.0001). The presence of increased CD14 expression on maturing granulocytic cells could be seen in cases with monosomy 7 in the setting of a normal blast percentage and when minimal (<3) other immunophenotypic abnormalities were seen on myeloid populations. CONCLUSIONS Increased CD14 expression on maturing granulocytic cells can be helpful in identifying patients with myeloid neoplasms who have monosomy 7. © 2019 International Clinical Cytometry Society.", "gemini": "BỐI CẢNH Các bất thường liên quan đến nhiễm sắc thể 7 là một trong những quang sai nhiễm sắc thể thường gặp nhất ở các bệnh tân sinh tủy bao gồm các hội chứng loạn sản tủy (MDS) và bệnh bạch cầu tủy cấp tính (AML) và có liên quan đến tiên lượng xấu. Miễn dịch học bằng phương pháp đo lưu lượng tế bào cung cấp dữ liệu có thể hỗ trợ trong chẩn đoán và phân loại các bệnh tân sinh tủy. Các đặc điểm miễn dịch học của các bệnh tân sinh tủy có tình trạng đơn nhiễm sắc thể 7 hoặc del(7q) trước đây chưa được mô tả một cách toàn diện. PHƯƠNG PHÁP Chúng tôi đã phân tích hồi cứu dữ liệu đo lưu lượng tế bào của các bệnh tân sinh tủy có tình trạng đơn nhiễm sắc thể 7 hoặc del(7q) và tóm tắt các đặc điểm miễn dịch học liên quan. KẾT QUẢ Các bệnh tân sinh tủy có tình trạng đơn nhiễm sắc thể 7 thường biểu hiện nhiều bất thường về miễn dịch học trên các tế bào nổ tủy và các tế bào tủy đơn bào đang trưởng thành. Sự gia tăng biểu hiện CD14 trên các tế bào hạt đang trưởng thành đặc biệt thấy ở các bệnh tân sinh tủy có tình trạng đơn nhiễm sắc thể 7. Bất thường này thường gặp hơn đáng kể ở các bệnh tân sinh tủy có tình trạng đơn nhiễm sắc thể 7 so với các bệnh có del(7q) (92,9% so với 8,2%, P < 0,0001). Sự gia tăng biểu hiện CD14 trên các tế bào hạt đang trưởng thành có thể thấy ở các trường hợp có tình trạng đơn nhiễm sắc thể 7 trong bối cảnh tỷ lệ nổ bình thường và khi thấy tối thiểu (<3) các bất thường về miễn dịch học khác trên quần thể tủy. KẾT LUẬN Sự gia tăng biểu hiện CD14 trên các tế bào hạt đang trưởng thành có thể hữu ích trong việc xác định những bệnh nhân mắc các bệnh tân sinh tủy có tình trạng đơn nhiễm sắc thể 7. © 2019 Hội lâm sàng đo lưu lượng tế bào quốc tế."} +{"idx": 1890085, "en": "OBJECTIVE The present study explores factors related to length of stay (LOS) in a rural public hospital in Thailand and assesses the feasibility of using LOS as an ED key performance indicator. METHODS Using a mixed-methods approach, qualitative methods (in-depth interviews, patients' chart review and participatory observations) were used to guide and elaborate findings from quantitative analysis of 555 electronic ED records. RESULTS Multivariate analysis revealed that age, Emergency Severity Index score and number of laboratory tests were significantly associated with LOS. The qualitative approach provided contradicting evidence on the linkage between LOS and patient outcomes. On the one hand, considering the 4 h rule, a child with asthma was referred to a tertiary care hospital because of deterioration after 4 h of ED care. On the other hand, a woman with sepsis was hospitalised with improved condition despite 7 h of ED care. Interviews revealed the waiting time to see doctors was probably the top priority issue for patients. CONCLUSIONS Factors related to LOS in a rural hospital in Thailand are similar and in contrast to those of a previous study in a medical school setting. Reasons for the discrepancy of findings and implications for improving ED services were discussed. Our data support the notion of controversy in using LOS as a key indicator of ED performance in this rural hospital setting. Thus, it is imperative to not rely on any single throughput or process indicators to monitor ED performance, but to take into account a set of indicators including patient outcomes.", "gemini": "MỤC TIÊU Nghiên cứu hiện tại khám phá các yếu tố liên quan đến thời gian nằm viện (LOS) tại một bệnh viện công ở vùng nông thôn ở Thái Lan và đánh giá tính khả thi của việc sử dụng LOS làm chỉ số hiệu suất chính của khoa cấp cứu. PHƯƠNG PHÁP Sử dụng phương pháp hỗn hợp, các phương pháp định tính (phỏng vấn chuyên sâu, xem xét biểu đồ bệnh nhân và quan sát có sự tham gia) đã được sử dụng để hướng dẫn và giải thích các phát hiện từ phân tích định lượng 555 hồ sơ cấp cứu điện tử. KẾT QUẢ Phân tích đa biến cho thấy tuổi, điểm Chỉ số mức độ nghiêm trọng của trường hợp cấp cứu và số lượng xét nghiệm là những yếu tố có liên quan đáng kể đến LOS. Phương pháp định tính cung cấp bằng chứng mâu thuẫn về mối liên hệ giữa LOS và kết quả của bệnh nhân. Một mặt, xét theo quy tắc 4 giờ, một đứa trẻ bị hen suyễn đã được chuyển đến bệnh viện chăm sóc chuyên sâu vì tình trạng xấu đi sau 4 giờ chăm sóc tại khoa cấp cứu. Mặt khác, một phụ nữ bị nhiễm trùng huyết đã được nhập viện với tình trạng được cải thiện mặc dù đã được chăm sóc tại khoa cấp cứu trong 7 giờ. Các cuộc phỏng vấn cho thấy thời gian chờ để gặp bác sĩ có lẽ là vấn đề được ưu tiên hàng đầu đối với bệnh nhân. KẾT LUẬN Các yếu tố liên quan đến LOS tại một bệnh viện ở vùng nông thôn ở Thái Lan tương tự và trái ngược với các yếu tố của một nghiên cứu trước đây trong bối cảnh trường y. Các lý do dẫn đến sự khác biệt về phát hiện và ý nghĩa đối với việc cải thiện các dịch vụ cấp cứu đã được thảo luận. Dữ liệu của chúng tôi ủng hộ quan điểm về tính gây tranh cãi khi sử dụng LOS làm chỉ số chính về hiệu suất của khoa cấp cứu trong bối cảnh bệnh viện ở vùng nông thôn này. Do đó, điều bắt buộc là không được dựa vào bất kỳ chỉ số thông lượng hoặc quy trình nào để theo dõi hiệu suất của khoa cấp cứu, mà phải tính đến một tập hợp các chỉ số bao gồm cả kết quả của bệnh nhân."} +{"idx": 1890086, "en": "The initiation of complementary feeding (CF; introducing infants to food/drink other than milk) is recommended close to 6 months and not before 4 months of age. Low socio-economic status (SES) is a determinant of nonadherence to CF recommendations, but there is an evidence gap around reasons for nonadherence among these parents. This study investigated knowledge, attitudes, and practices of disadvantaged families (in terms of SES and social support) and use of guidance for CF, in the Republic of Ireland and Northern Ireland. Parents of infants aged 3-14 months were recruited via community groups. Semistructured focus groups aided by vignettes were used. Data were analysed using an inductive thematic approach. Nineteen focus groups took place with parents (n = 83). A range of factors influence parents when introducing solids. Sources of guidance extend to family, friends, the internet, and commercial resources. Parents experience uncertainty and anxiety during this time, driven by lack of knowledge and conflicting advice. Five major themes were identified: (a) more guidance that is accessible, timely, and respectfully needed; (b) the challenge of choosing safe, nutritious food; (c) \"everybody has an opinion\"; (d) feelings of inadequacy, embarrassment, and guilt; and (e) decisions are ultimately based on individual circumstances. CF advice should be culturally appropriate, practical, and empowering, emphasising the rationale behind updates to recommendations and consequences of nonadherence. Future training of health professionals for delivery of CF advice and guidance should consider these findings. Compliance with CF recommendations is influenced by health professionals, the wider family, and the commercial baby-food sector.", "gemini": "Việc bắt đầu ăn bổ sung (CF; cho trẻ ăn thức ăn/đồ uống khác ngoài sữa) được khuyến nghị gần 6 tháng và không sớm hơn 4 tháng tuổi. Trạng thái kinh tế xã hội thấp (SES) là một yếu tố quyết định việc không tuân thủ các khuyến nghị về CF, nhưng vẫn còn một khoảng trống bằng chứng xung quanh lý do không tuân thủ ở những bậc cha mẹ này. Nghiên cứu này đã điều tra kiến thức, thái độ và thực hành của các gia đình khó khăn (về mặt SES và hỗ trợ xã hội) và việc sử dụng hướng dẫn cho CF tại Cộng hòa Ireland và Bắc Ireland. Cha mẹ của trẻ sơ sinh từ 3-14 tháng tuổi đã được tuyển dụng thông qua các nhóm cộng đồng. Các nhóm thảo luận chuyên sâu có sự hỗ trợ của hình ảnh minh họa đã được sử dụng. Dữ liệu được phân tích bằng phương pháp tiếp cận chủ đề quy nạp. Mười chín nhóm thảo luận chuyên sâu đã diễn ra với sự tham gia của cha mẹ (n = 83). Một loạt các yếu tố ảnh hưởng đến cha mẹ khi cho trẻ ăn dặm. Các nguồn hướng dẫn mở rộng đến gia đình, bạn bè, internet và các nguồn thương mại. Cha mẹ cảm thấy không chắc chắn và lo lắng trong thời gian này, do thiếu kiến thức và lời khuyên mâu thuẫn. Năm chủ đề chính đã được xác định: (a) cần có thêm hướng dẫn dễ tiếp cận, kịp thời và tôn trọng; (b) thách thức trong việc lựa chọn thực phẩm an toàn, bổ dưỡng; (c) \"mọi người đều có ý kiến\"; (d) cảm giác không đủ năng lực, xấu hổ và tội lỗi; và (e) các quyết định cuối cùng dựa trên hoàn cảnh cá nhân. Lời khuyên về CF phải phù hợp với văn hóa, thiết thực và mang tính trao quyền, nhấn mạnh lý do đằng sau các cập nhật đối với các khuyến nghị và hậu quả của việc không tuân thủ. Đào tạo trong tương lai của các chuyên gia y tế về việc đưa ra lời khuyên và hướng dẫn về CF nên xem xét những phát hiện này. Việc tuân thủ các khuyến nghị về CF chịu ảnh hưởng của các chuyên gia y tế, gia đình rộng hơn và ngành thực phẩm dành cho trẻ em thương mại."} +{"idx": 1890087, "en": "A new and sustainable process was reported for the in situ synthesis of Nb-Pb co-doped and Pd-loaded TiO2 /BaTiO3 nanometer heterostructures from waste multilayer ceramic capacitors through a simple chlorination-leaching route. The particle size of the Nb-Pb co-doped heterostructure and the Pd loading were 20-50 nm and less than 5 nm, respectively. The bandgaps of the prepared samples were in the range 2.81-2.92 eV. The optimal simulated-sunlight photocatalytic H2 production rate and Rhodamine B degradation rate of the prepared heterostructure could reach 576.8 μmol g-1  h-1 and 0.29911 min-1 , respectively, which were approximately 11.3 and 19.1 times higher than those of commercial TiO2 , and 5.96 and 8.91 times higher than those of bare TiO2 /BaTiO3 . The recycled heterostructure exhibited excellent photostability and reusability. Such superior photocatalytic performance of the sample was attributed to the formation of the heterostructure, the Nb-Pb co-doping, and the Pd loading, which enhanced the visible light absorption and charge separation efficiency. Furthermore, DFT calculations were applied to explore the enhanced mechanism. This study demonstrates a sustainable process for the conversion of e-waste to a high-value-added and highefficiency photocatalyst, which has the advantages of waste utilization, low-cost preparation, and environmental protection.", "gemini": "Một quy trình mới và bền vững đã được báo cáo để tổng hợp tại chỗ các cấu trúc dị thể nano TiO2 / BaTiO3 đồng pha tạp Nb-Pb và tải Pd từ tụ điện gốm nhiều lớp phế thải thông qua một lộ trình clo hóa-chiết tách đơn giản. Kích thước hạt của cấu trúc dị thể đồng pha tạp Nb-Pb và lượng Pd tải lần lượt là 20-50 nm và nhỏ hơn 5 nm. Băng cấm của các mẫu đã chuẩn bị nằm trong khoảng 2,81-2,92 eV. Tốc độ sản xuất quang xúc tác H2 tối ưu mô phỏng ánh sáng mặt trời và tốc độ phân hủy Rhodamine B của cấu trúc dị thể đã chuẩn bị có thể đạt lần lượt là 576,8 μmol g-1 h-1 và 0,29911 phút-1, cao hơn khoảng 11,3 và 19,1 lần so với TiO2 thương mại, và cao hơn 5,96 và 8,91 lần so với TiO2 / BaTiO3 trần. Cấu trúc dị thể tái chế cho thấy tính ổn định quang và khả năng tái sử dụng tuyệt vời. Hiệu suất quang xúc tác vượt trội như vậy của mẫu được quy cho sự hình thành của cấu trúc dị thể, việc đồng pha tạp Nb-Pb và việc tải Pd, giúp tăng cường hấp thụ ánh sáng nhìn thấy và hiệu suất tách điện tích. Hơn nữa, các tính toán DFT đã được áp dụng để khám phá cơ chế nâng cao. Nghiên cứu này chứng minh một quy trình bền vững để chuyển đổi rác thải điện tử thành chất xúc tác quang có giá trị gia tăng cao và hiệu suất cao, có những ưu điểm về tái sử dụng chất thải, chế biến chi phí thấp và bảo vệ môi trường."} +{"idx": 1890088, "en": "Vast amounts of carbon are bound in both active layer and permafrost soils in the Arctic. As a consequence of climate warming, the depth of the active layer is increasing in size and permafrost soils are thawing. We hypothesize that pulses of biogenic volatile organic compounds are released from the near-surface active layer during spring, and during late summer season from thawing permafrost, while the subsequent biogeochemical processes occurring in thawed soils also lead to emissions. Biogenic volatile organic compounds are reactive gases that have both negative and positive climate forcing impacts when introduced to the Arctic atmosphere, and the knowledge of their emission magnitude and pattern is necessary to construct reliable climate models. However, it is unclear how different ecosystems and environmental factors such as drainage conditions upon permafrost thaw affect the emission and compound composition. Here we show that incubations of frozen B horizon of the active layer and permafrost soils collected from a High Arctic heath and fen release a range of biogenic volatile organic compounds upon thaw and during subsequent incubation experiments at temperatures of 10°C and 20°C. Meltwater drainage in the fen soils increased emission rates nine times, while having no effect in the drier heath soils. Emissions generally increased with temperature, and emission profiles for the fen soils were dominated by benzenoids and alkanes, while benzenoids, ketones, and alcohols dominated in heath soils. Our results emphasize that future changes affecting the drainage conditions of the Arctic tundra will have a large influence on volatile emissions from thawing permafrost soils - particularly in wetland/fen areas.", "gemini": "Một lượng lớn carbon được liên kết trong cả lớp hoạt động và đất đóng băng vĩnh cửu ở Bắc Cực. Do hậu quả của sự nóng lên toàn cầu, độ sâu của lớp hoạt động đang tăng lên về kích thước và đất đóng băng vĩnh cửu đang tan băng. Chúng tôi đưa ra giả thuyết rằng các xung hợp chất hữu cơ dễ bay hơi sinh học được giải phóng từ lớp hoạt động gần bề mặt vào mùa xuân và vào cuối mùa hè từ lớp đất đóng băng vĩnh cửu đang tan, trong khi các quá trình địa hóa sinh tiếp theo xảy ra trong đất tan băng cũng dẫn đến phát thải. Các hợp chất hữu cơ dễ bay hơi sinh học là những khí phản ứng có tác động cưỡng bức khí hậu cả tiêu cực và tích cực khi được đưa vào khí quyển Bắc Cực và kiến thức về cường độ và kiểu phát thải của chúng là cần thiết để xây dựng các mô hình khí hậu đáng tin cậy. Tuy nhiên, vẫn chưa rõ các hệ sinh thái khác nhau và các yếu tố môi trường như điều kiện thoát nước khi lớp đất đóng băng vĩnh cửu tan ảnh hưởng như thế nào đến phát thải và thành phần hợp chất. Ở đây, chúng tôi chỉ ra rằng việc ủ chân trời B đóng băng của lớp hoạt động và đất đóng băng vĩnh cửu thu thập được từ một vùng đất hoang và đầm lầy ở Bắc Cực cao giải phóng một loạt các hợp chất hữu cơ dễ bay hơi sinh học khi tan băng và trong các thí nghiệm ủ tiếp theo ở nhiệt độ 10°C và 20°C. Thoát nước tan chảy ở đất đầm lầy làm tăng tốc độ phát thải gấp chín lần, trong khi không có tác dụng ở đất hoang khô hơn. Phát thải nói chung tăng theo nhiệt độ và các cấu hình phát thải đối với đất đầm lầy chủ yếu là benzenoid và ankan, trong khi benzenoid, xeton và rượu chiếm ưu thế ở đất hoang. Kết quả của chúng tôi nhấn mạnh rằng những thay đổi trong tương lai ảnh hưởng đến điều kiện thoát nước của vùng lãnh nguyên Bắc Cực sẽ có ảnh hưởng lớn đến lượng phát thải dễ bay hơi từ đất đóng băng vĩnh cửu đang tan - đặc biệt là ở các khu vực đất ngập nước/đầm lầy."} +{"idx": 1890089, "en": "We have reported the photochemical regulation of the intracellular antisense effect of antisense probes containing a photo-responsive artificial nucleic acid, 3-cyanovinylcarbazole nucleoside (CNV K). Here we focus on the importance of the photocrosslinking rate on the inhibitory effect on gene expression using photocrosslinkable antisense probes (pcASOs). The inhibitory effect of pcASOs on GFP gene expression was dependent on the photocrosslinking rate of 3-cyanovinylcarbazole with d-threoninol (CNV D), CNV K, or psoralen. The ultrafast RNA photocrosslinking induced the formation of a thermally irreversible covalent bond between pcASOs and the target RNA. These ASOs strongly inhibited gene expression only when the photocrosslinking rate was faster than the random walk of branch migration. In addition, pcASOs containing CNV D or CNV K targeted the RNAs with secondary structures. These results indicate the regulatory effect of photocrosslinker and photoirradiation energy using pcASOs on the gene expression level.", "gemini": "Chúng tôi đã báo cáo về sự điều chỉnh quang hóa của hiệu ứng chống lại nghĩa của các đầu dò chống lại nghĩa chứa axit nucleic nhân tạo phản ứng với ánh sáng, nucleoside 3-cyanovinylcarbazole (CNV K). Ở đây, chúng tôi tập trung vào tầm quan trọng của tốc độ liên kết chéo quang đối với hiệu ứng ức chế biểu hiện gen bằng cách sử dụng các đầu dò chống lại nghĩa có thể liên kết chéo quang (pcASO). Hiệu ứng ức chế của pcASO đối với biểu hiện gen GFP phụ thuộc vào tốc độ liên kết chéo quang của 3-cyanovinylcarbazole với d-threoninol (CNV D), CNV K hoặc psoralen. Liên kết chéo quang RNA cực nhanh gây ra sự hình thành liên kết cộng hóa trị không thể đảo ngược nhiệt giữa pcASO và RNA đích. Các ASO này ức chế mạnh biểu hiện gen chỉ khi tốc độ liên kết chéo quang nhanh hơn sự di chuyển ngẫu nhiên của quá trình di chuyển nhánh. Ngoài ra, pcASO chứa CNV D hoặc CNV K nhắm vào các RNA có cấu trúc thứ cấp. Những kết quả này chỉ ra hiệu ứng điều hòa của chất liên kết chéo quang và năng lượng chiếu xạ quang bằng cách sử dụng pcASO ở mức biểu hiện gen."} +{"idx": 1890090, "en": "Endoglin (ENG) regulates signaling by transforming growth factor-β (TGF-β), a genetic modifier of cystic fibrosis (CF) lung disease severity. We hypothesized that ENG mediates TGF-β pathobiology in CF airway epithelia. Comparing CF and non-CF human lungs, we measured ENG by qPCR, immunoblotting and ELISA. In human bronchial epithelial cell lines (16HBE), we used CFTR siRNA knockdown and functional inhibition (CFTRINH -172) to connect loss of CFTR to ENG synthesis. Plasmid overexpression of ENG assessed the direct effect of ENG on TGF-β transcription and signal amplification in 16HBE cells. We found ENG protein to be increased more than fivefold both in human CF bronchoalveolar fluid (BALF) and human CF lung homogenates. ENG transcripts were increased threefold in CF, with a twofold increase in TGF-β signaling. CFTR knockdown in 16HBE cells tripled ENG transcription and doubled protein levels with corresponding increases in TGF-β signaling. Plasmid overexpression of ENG alone nearly doubled TGF-β1 mRNA and increased TGF-β signaling in 16HBE cells. These experiments identify that loss of CFTR function increases ENG expression in CF epithelia and amplifies TGF-β signaling. Targeting ENG may offer a novel therapeutic opportunity to address TGF-β associated pathobiology in CF.", "gemini": "Endoglin (ENG) điều chỉnh tín hiệu bằng cách chuyển đổi yếu tố tăng trưởng-β (TGF-β), một chất điều chỉnh di truyền của mức độ nghiêm trọng của bệnh phổi xơ nang (CF). Chúng tôi đưa ra giả thuyết rằng ENG trung gian cho bệnh lý TGF-β trong biểu mô đường thở của CF. So sánh phổi của người bị CF và không bị CF, chúng tôi đã đo ENG bằng qPCR, miễn dịch và ELISA. Trong các dòng tế bào biểu mô phế quản của người (16HBE), chúng tôi đã sử dụng ARN can thiệp CFTR và ức chế chức năng (CFTRINH -172) để kết nối sự mất CFTR với tổng hợp ENG. Plasmid biểu hiện quá mức ENG đánh giá tác dụng trực tiếp của ENG đối với phiên mã TGF-β và khuếch đại tín hiệu trong các tế bào 16HBE. Chúng tôi thấy rằng protein ENG tăng hơn gấp năm lần trong cả dịch phế nang phế quản CF của người (BALF) và dịch đồng nhất phổi CF của người. Các bản sao ENG tăng gấp ba lần ở CF, trong khi tín hiệu TGF-β tăng gấp đôi. ARN can thiệp CFTR ở các tế bào 16HBE tăng gấp ba lần phiên mã ENG và tăng gấp đôi mức protein với sự gia tăng tương ứng trong tín hiệu TGF-β. Chỉ riêng plasmid biểu hiện quá mức ENG đã tăng gần gấp đôi mRNA TGF-β1 và tăng tín hiệu TGF-β trong các tế bào 16HBE. Những thí nghiệm này xác định rằng mất chức năng CFTR làm tăng biểu hiện ENG trong biểu mô CF và khuếch đại tín hiệu TGF-β. Nhắm mục tiêu vào ENG có thể mang lại cơ hội điều trị mới để giải quyết bệnh lý liên quan đến TGF-β trong CF."} +{"idx": 1890091, "en": "In diabetes mellitus (DM), the kidneys are exposed to increased levels of hyperglycemia-induced oxidative stress. Elevated amounts of reactive oxygen species (ROS) are believed to provoke ultrastructural changes in kidney tissue and can eventually result in DM late complications such as diabetic nephropathy. While it is reported that glucagon-like peptide 1 receptors (GLP-1R) are present in the kidney vasculature, the effects of GLP-1 on the kidney proteome in DM is not well described. Thus, we set out to investigate potential effects on the proteomic level. Here the effects of GLP-1R agonism using the GLP-1 analogue liraglutide are studied in the kidneys of streptozotocin (STZ)-treated mice (n = 6/group) by label-free shotgun mass spectrometry (MS) and targeted MS. Unsupervised and supervised multivariate analyses are followed by one-way ANOVA. Shotgun MS data of vehicle and liraglutide-treated mouse groups are separated in the supervised multivariate analysis and separation is also achieved in the subsequent unsupervised multivariate analysis using targeted MS data. The mouse group receiving the GLP-1R agonist liraglutide has increased protein abundances of glutathione peroxidase-3 (GPX3) and catalase (CATA) while the abundances of neuroplastin (NPTN) and bifunctional glutamate/proline-tRNA ligase (SYEP) are decreased compared to the STZ vehicle mice. The data suggest that GLP-1R agonism mainly influences abundances of structurally involved proteins and proteins involved in oxidative stress responses in the STZ mouse kidney. The changes could be direct effects of GLP-1R agonism in the kidneys or indirectly caused by a systemic response to GLP-1R activation.", "gemini": "Trong bệnh tiểu đường (ĐT), thận phải tiếp xúc với mức độ căng thẳng oxy hóa do tăng đường huyết. Người ta tin rằng lượng lớn các loại oxy phản ứng (ROS) gây ra những thay đổi về cấu trúc siêu nhỏ trong mô thận và cuối cùng có thể dẫn đến các biến chứng muộn của ĐT như bệnh thận do tiểu đường. Mặc dù có báo cáo rằng các thụ thể peptide giống glucagon-1 (GLP-1R) có trong mạch máu thận, nhưng tác dụng của GLP-1 đối với proteome thận ở ĐT vẫn chưa được mô tả rõ. Do đó, chúng tôi tiến hành điều tra các tác động tiềm tàng ở cấp độ proteome. Ở đây, các tác động của chủ vận GLP-1R sử dụng chất tương tự GLP-1 liraglutide được nghiên cứu ở thận của chuột được điều trị bằng streptozotocin (STZ) (n = 6/nhóm) bằng phương pháp quang phổ khối súng ngắn không đánh dấu (MS) và MS có mục tiêu. Các phân tích đa biến không có giám sát và có giám sát được theo sau bởi ANOVA một chiều. Dữ liệu MS súng ngắn của các nhóm chuột được xử lý bằng dung môi và liraglutide được tách riêng trong phân tích đa biến có giám sát và sự tách biệt cũng đạt được trong phân tích đa biến không có giám sát tiếp theo bằng cách sử dụng dữ liệu MS có mục tiêu. Nhóm chuột nhận được chất chủ vận GLP-1R liraglutide có sự gia tăng về lượng protein glutathione peroxidase-3 (GPX3) và catalase (CATA) trong khi lượng neuroplastin (NPTN) và bifunctional glutamate/proline-tRNA ligase (SYEP) giảm so với chuột đối chứng STZ. Dữ liệu cho thấy rằng chủ vận GLP-1R chủ yếu ảnh hưởng đến sự phong phú của các protein có liên quan đến cấu trúc và các protein liên quan đến phản ứng căng thẳng oxy hóa trong thận chuột STZ. Những thay đổi có thể là tác dụng trực tiếp của chủ vận GLP-1R ở thận hoặc do phản ứng toàn thân với hoạt hóa GLP-1R gây ra."} +{"idx": 1890092, "en": "Cornelia de Lange syndrome (CdLS) is an autosomal dominant genetic disorder caused by pathogenic variants in NIPBL, RAD21, SMC3, HDAC8, or SMC1A; all of which code for proteins that are components of, or interact with, the cohesin complex. Despite the identification of multiple genes associated with CdLS, over 25% of individuals strongly suspected to have CdLS have negative genetic testing, indicating that there are additional genes associated with the condition. HDAC2 codes for histone deacetylase 2 (HDAC2) and, like HDAC8, is a Class 1 histone deacetylase. We present a patient with a novel de novo variant in HDAC2 with many clinical features consistent with CdLS including severe developmental delay, limb abnormalities, congenital heart defect, cryptorchidism and hypoplastic genitalia, growth retardation, and characteristic craniofacial features. Although variants in HDAC2 are not currently associated with human disease, the variant identified in this patient is within a highly conserved amino acid residue and has not been observed in healthy populations. This information, along with the patient's clinical presentation and the functional similarity between the HDAC2 and HDAC8 proteins, suggests that HDAC2 should be further investigated as a candidate gene for CdLS or a CdLS-like syndrome.", "gemini": "Hội chứng Cornelia de Lange (CdLS) là một rối loạn di truyền trội trên nhiễm sắc thể thường do các biến thể gây bệnh ở NIPBL, RAD21, SMC3, HDAC8 hoặc SMC1A; tất cả đều mã hóa các protein là thành phần của hoặc tương tác với phức hợp cohesin. Mặc dù đã xác định được nhiều gen liên quan đến CdLS, nhưng hơn 25% số cá nhân được nghi ngờ mắc CdLS có kết quả xét nghiệm di truyền âm tính, cho thấy rằng có thêm các gen khác liên quan đến tình trạng này. HDAC2 mã hóa cho histone deacetylase 2 (HDAC2) và giống như HDAC8, là một histone deacetylase loại 1. Chúng tôi trình bày một bệnh nhân có biến thể de novo mới ở HDAC2 với nhiều đặc điểm lâm sàng phù hợp với CdLS bao gồm chậm phát triển nghiêm trọng, bất thường về chi, dị tật tim bẩm sinh, tinh hoàn ẩn và cơ quan sinh dục nhỏ, chậm phát triển và các đặc điểm đặc trưng trên khuôn mặt. Mặc dù các biến thể ở HDAC2 hiện không liên quan đến bệnh ở người, nhưng biến thể được xác định ở bệnh nhân này nằm trong một phần tử axit amin được bảo tồn cao và chưa được quan sát thấy ở quần thể khỏe mạnh. Thông tin này, cùng với biểu hiện lâm sàng của bệnh nhân và sự giống nhau về chức năng giữa các protein HDAC2 và HDAC8, cho thấy rằng HDAC2 nên được nghiên cứu sâu hơn như một gen ứng viên cho CdLS hoặc một hội chứng giống CdLS."} +{"idx": 1890093, "en": "OBJECTIVE To identify discharge medications, especially psychotropic medications that are associated with falls, amongst older adults within 6 months following hospitalisation. METHODS Negative binomial regression was used to examine relationships between discharge medications and falls in older post-hospitalised adults. Multiple regression that considered falls risk factors at discharge was performed. RESULTS Data for 267 participants showed that discharge medications were not independently associated with falls postdischarge after adjustment for other falls risk factors. Male gender (adjusted incidence rate ratio [95% confidence interval, CI]) 2.15 [1.36-3.40]), higher depression scores (1.14 [1.05-1.25]) and co-morbidity of neurological disease other than stroke (5.98 [3.08-11.60]) were independently associated with an increased rate of falls. Higher depression scores (1.20 [1.11-1.31]) and co-morbidity of cancer (1.97 [1.20-3.25]) were independently associated with an increased rate of injurious falls in the 6 months postdischarge. CONCLUSION Falls prevention strategies, other than hospital discharge medication management in the postdischarge older adults, warrant investigation.", "gemini": "MỤC TIÊU Xác định các loại thuốc xuất viện, đặc biệt là các loại thuốc hướng thần có liên quan đến tình trạng ngã ở người cao tuổi trong vòng 6 tháng sau khi nhập viện. PHƯƠNG PHÁP Phân hồi hồi quy nhị thức âm được sử dụng để kiểm tra mối quan hệ giữa các loại thuốc xuất viện và tình trạng ngã ở người cao tuổi sau khi xuất viện. Phân hồi hồi quy bội được thực hiện để xem xét các yếu tố nguy cơ ngã khi xuất viện. KẾT QUẢ Dữ liệu của 267 người tham gia cho thấy các loại thuốc xuất viện không liên quan độc lập đến tình trạng ngã sau khi xuất viện sau khi điều chỉnh các yếu tố nguy cơ ngã khác. Giới tính nam (tỷ lệ mắc bệnh đã điều chỉnh [khoảng tin cậy 95%, CI]) 2,15 [1,36-3,40]), điểm trầm cảm cao hơn (1,14 [1,05-1,25]) và bệnh lý thần kinh đi kèm khác ngoài đột quỵ (5,98 [3,08-11,60]) có liên quan độc lập với tỷ lệ ngã tăng. Điểm trầm cảm cao hơn (1,20 [1,11-1,31]) và bệnh ung thư đi kèm (1,97 [1,20-3,25]) có liên quan độc lập với tỷ lệ ngã gây thương tích tăng trong 6 tháng sau khi xuất viện. KẾT LUẬN Các chiến lược phòng ngừa ngã, ngoài việc quản lý thuốc xuất viện ở người cao tuổi sau khi xuất viện, cần được điều tra."} +{"idx": 1890094, "en": "BACKGROUND Although cigarette smoking is considered one of the key risk factors for lung cancer, 15% of male patients and 53% of female patients with lung cancer are non-smokers. Metabolic changes are critical features of cancer. Therapeutic target identification from a metabolic perspective in non-small cell lung cancer (NSCLC) tissue of female non-smokers has long been ignored. RESULTS Based on microarray data retrieved from Affymetrix expression arrays E-GEOD-19804, we found that the downregulated genes in non-smoking female NSCLC patients tended to participate in protein/amino acid and lipid metabolism, while upregulated genes were more involved in protein/amino acid and carbohydrate metabolism. Combining nutrient metabolic co-expression, protein-protein interaction network construction and overall survival assessment, we identified NR4A1 and TIE1 as potential therapeutic targets for NSCLC in female non-smokers. To accelerate the drug development for non-smoking female NSCLC patients, we identified nilotinib as a potential agonist targeting NR4A1 encoded protein by molecular docking and molecular dynamic stimulation. We also show that nilotinib inhibited proliferation and induced senescence of cells in non-smoking female NSCLC patients in vitro. CONCLUSIONS These results not only uncover nutrient metabolic characteristics in non-smoking female NSCLC patients, but also provide a new paradigm for identifying new targets and drugs for novel therapy for such patients.", "gemini": "BỐI CẢNH Mặc dù hút thuốc lá được coi là một trong những yếu tố nguy cơ chính gây ung thư phổi, 15% bệnh nhân nam và 53% bệnh nhân nữ mắc ung thư phổi không phải là người hút thuốc. Những thay đổi về chuyển hóa là đặc điểm quan trọng của bệnh ung thư. Việc xác định mục tiêu điều trị từ góc độ chuyển hóa ở mô ung thư phổi không tế bào nhỏ (NSCLC) của phụ nữ không hút thuốc từ lâu đã bị bỏ qua. KẾT QUẢ Dựa trên dữ liệu mảng vi mô thu được từ mảng biểu hiện Affymetrix E-GEOD-19804, chúng tôi thấy rằng các gen bị điều hòa giảm ở bệnh nhân NSCLC nữ không hút thuốc có xu hướng tham gia vào quá trình chuyển hóa protein/axit amin và lipid, trong khi các gen được điều hòa tăng lại liên quan nhiều hơn đến quá trình chuyển hóa protein/axit amin và carbohydrate. Kết hợp biểu hiện đồng thời của quá trình chuyển hóa chất dinh dưỡng, xây dựng mạng lưới tương tác protein-protein và đánh giá khả năng sống sót chung, chúng tôi xác định NR4A1 và TIE1 là các mục tiêu điều trị tiềm năng cho NSCLC ở phụ nữ không hút thuốc. Để đẩy nhanh quá trình phát triển thuốc cho bệnh nhân NSCLC nữ không hút thuốc, chúng tôi đã xác định nilotinib là một chất chủ vận tiềm năng nhắm vào protein được mã hóa NR4A1 bằng cách ghép nối phân tử và kích thích động lực phân tử. Chúng tôi cũng chỉ ra rằng nilotinib ức chế sự tăng sinh và gây ra tình trạng lão hóa của tế bào ở bệnh nhân NSCLC nữ không hút thuốc trong ống nghiệm. KẾT LUẬN Những kết quả này không chỉ khám phá ra các đặc điểm chuyển hóa chất dinh dưỡng ở bệnh nhân NSCLC nữ không hút thuốc mà còn cung cấp một mô hình mới để xác định các mục tiêu và loại thuốc mới cho liệu pháp mới cho những bệnh nhân như vậy."} +{"idx": 1890095, "en": "Owing to energy shortages and environmental pollution, green energy sources such as polymer electrolyte fuel cells and metal-air batteries play a more and more important role, whereby the oxygen reduction reaction (ORR) is the rate-determining step. Development of high-efficiency and stable catalysts to facilitate the ORR is of importance. Graphene is a new type of material with two-dimensional structure and high surface area, which has wide-ranging applications in many fields. However, graphene with zero band gap shows low electrocatalytic activity toward the ORR. Introduction of nonmetal atoms can change the electronic arrangement, generate active sites, and further improve the catalytic activity of graphene. Some nonmetal-doping strategies (e.g., N, S, and P doping) can promote ORR activity. Herein, the recent development of nonmetal-doped graphene catalysts for ORR is reviewed. Some common synthetic methods for nonmetal-doped graphene materials are summarized, and the active sites and possible reaction mechanisms for ORR on various nonmetal-doped graphene catalysts are discussed.", "gemini": "Do tình trạng thiếu năng lượng và ô nhiễm môi trường, các nguồn năng lượng xanh như pin nhiên liệu điện phân tử polyme và pin kim loại-không khí đóng vai trò ngày càng quan trọng, trong đó phản ứng khử oxy (ORR) là bước quyết định tốc độ. Phát triển các chất xúc tác hiệu suất cao và ổn định để tạo thuận lợi cho ORR là rất quan trọng. Graphene là một loại vật liệu mới có cấu trúc hai chiều và diện tích bề mặt lớn, có ứng dụng rộng rãi trong nhiều lĩnh vực. Tuy nhiên, graphene có khoảng trống băng bằng 0 cho thấy hoạt tính điện xúc tác thấp đối với ORR. Việc đưa các nguyên tử phi kim loại vào có thể thay đổi sự sắp xếp điện tử, tạo ra các vị trí hoạt động và cải thiện thêm hoạt tính xúc tác của graphene. Một số chiến lược ghép phi kim loại (ví dụ: ghép N, S và P) có thể thúc đẩy hoạt động ORR. Tại đây, chúng tôi xem xét sự phát triển gần đây của các chất xúc tác graphene được ghép phi kim loại cho ORR. Một số phương pháp tổng hợp phổ biến đối với vật liệu graphene được ghép phi kim loại được tóm tắt, và các vị trí hoạt động và cơ chế phản ứng có thể xảy ra đối với ORR trên các chất xúc tác graphene được ghép phi kim loại khác nhau được thảo luận."} +{"idx": 1890096, "en": "Exercise can improve morbidity and mortality in heart failure patients; however, the underlying mechanisms remain to be fully investigated. Thus, we investigated the effects of exercise on cardiac function and ventricular arrhythmias in myocardial infarction (MI) induced heart failure mice. Wild-type male mice underwent sham-operation or permanent left coronary artery ligation to induce MI. MI mice were divided into a sedentary (MI-Sed) and two intervention groups: MI-Ex (underwent 6-week treadmill exercise training) and MI-βb (oral bisoprolol treatment (1 mg/kg/d) without exercise). Cardiac function and structure were assessed by echocardiography and histology. Exercise capacity and cardiopulmonary function was accepted as oxygen consumption at peak exercise (peak VO2 ). Autonomic nervous system function and the incidence of spontaneous ventricular arrhythmia were evaluated via telemetry recording. mRNA and protein expressions in the left ventricle (LV) were investigated by real-time PCR and Western blotting. There were no differences in survival rate, MI size, cardiac function and structure, while exercise training improved peak VO2 . Compared with MI-Sed, MI-Ex, and MI-βb showed decreased sympathetic tone and lower incidence of spontaneous ventricular arrhythmia. By Western blot, the hyperphosphorylation of CaMKII and RyR2 were restored by exercise and β-blocker treatment. Furthermore, elevated expression of miR-1 and decreased expression of its target protein PP2A were recovered by exercise and β-blocker treatment. Continuous intensive exercise training can suppress ventricular arrhythmias in subacute to chronic phase of MI through restoring autonomic imbalance and impaired calcium handling, similarly to that for β-blockers.", "gemini": "Tập thể dục có thể cải thiện tỷ lệ mắc bệnh và tử vong ở những bệnh nhân suy tim; tuy nhiên, các cơ chế cơ bản vẫn chưa được nghiên cứu đầy đủ. Do đó, chúng tôi đã nghiên cứu các tác động của việc tập thể dục đối với chức năng tim và loạn nhịp thất ở những con chuột suy tim do nhồi máu cơ tim (MI). Những con chuột đực bình thường đã trải qua phẫu thuật giả hoặc thắt động mạch vành trái vĩnh viễn để gây nhồi máu cơ tim. Những con chuột nhồi máu cơ tim được chia thành nhóm ít vận động (MI-Sed) và hai nhóm can thiệp: MI-Ex (đã trải qua 6 tuần tập luyện trên máy chạy bộ) và MI-βb (điều trị bisoprolol đường uống (1 mg/kg/ngày) mà không tập thể dục). Chức năng và cấu trúc của tim được đánh giá bằng siêu âm tim và mô học. Khả năng tập thể dục và chức năng tim phổi được chấp nhận là mức tiêu thụ oxy khi tập thể dục ở mức cao nhất (VO2 đỉnh). Chức năng hệ thần kinh tự chủ và tỷ lệ loạn nhịp thất tự phát được đánh giá thông qua ghi từ xa. Biểu hiện mRNA và protein ở tâm thất trái (LV) được nghiên cứu bằng phản ứng chuỗi polymerase thời gian thực và phương pháp Western blotting. Không có sự khác biệt về tỷ lệ sống sót, kích thước nhồi máu cơ tim, chức năng và cấu trúc tim, trong khi tập luyện thể dục cải thiện VO2 đỉnh. So với MI-Sed, MI-Ex và MI-βb cho thấy giảm trương lực giao cảm và tỷ lệ loạn nhịp thất tự phát thấp hơn. Theo phương pháp Western blot, sự tăng phosphoryl hóa của CaMKII và RyR2 được phục hồi bằng cách tập thể dục và điều trị bằng thuốc chẹn β. Hơn nữa, biểu hiện tăng của miR-1 và biểu hiện giảm của protein đích PP2A của nó được phục hồi bằng cách tập thể dục và điều trị bằng thuốc chẹn β. Tập luyện thể dục cường độ cao liên tục có thể ức chế loạn nhịp thất ở giai đoạn bán cấp đến mãn tính của nhồi máu cơ tim thông qua việc phục hồi mất cân bằng tự chủ và xử lý canxi bị suy yếu, tương tự như thuốc chẹn β."} +{"idx": 1890097, "en": "OBJECTIVES To examine the mechanisms of coping strategies on nurses' psychological well-being, practice environments and safety attitudes. METHODS A cross-sectional study design was used. Structural equation modeling was performed to analyze the results. Five hundred clinical nurses were randomly selected from a large group of 1,500 from a medical center with 1,350 beds in Taipei, Taiwan, from July to October 2015. Self-report questionnaires were administered to measure coping strategies (Brief COPE), psychological well-being (Ryff's Psychological Well-being Scale), nurses' practice environments (Practice Environment Scale of the Nursing Work Index), and safety attitudes (Safety Attitudes Questionnaire). RESULTS Of the 500 participants who gave written consent, 474 (94.8%) filled out the questionnaire. Results showed that using more approach-oriented coping strategies and fewer avoidant coping strategies was associated with greater psychological well-being. Psychological well-being was directly associated with quality of nurses' practice environments and safety attitudes. The impact of psychological well-being on safety attitudes was mediated significantly by the quality of the practice environment. The use of approach-oriented coping strategies was significantly predictive of positive psychological well-being, a good practice environment, and good safety attitudes. CONCLUSIONS AND CLINICAL RELEVANCE This study found a distinct pathway for the relationships between clinical nurses' psychological well-being, practice environment, and safety attitudes. Psychological well-being in clinical nurses was higher for those with more approach-oriented coping strategies. Psychological well-being directly impacted safety attitudes, which mediated nurses' practice environments. The practical implications of the results suggest that interventions designed to promote positive psychological well-being may help improve nurses' practice environments, which, in turn, may result in better safety attitudes and nursing care outcomes.", "gemini": "MỤC TIÊU Nghiên cứu các cơ chế của các chiến lược đối phó đối với sức khỏe tâm lý, môi trường thực hành và thái độ an toàn của y tá. PHƯƠNG PHÁP Một thiết kế nghiên cứu cắt ngang đã được sử dụng. Mô hình phương trình cấu trúc được thực hiện để phân tích kết quả. Năm trăm y tá lâm sàng được chọn ngẫu nhiên từ một nhóm lớn gồm 1.500 y tá từ một trung tâm y tế có 1.350 giường tại Đài Bắc, Đài Loan, từ tháng 7 đến tháng 10 năm 2015. Các bảng câu hỏi tự báo cáo đã được sử dụng để đo lường các chiến lược đối phó (Brief COPE), sức khỏe tâm lý (Thang sức khỏe tâm lý của Ryff), môi trường thực hành của y tá (Thang môi trường thực hành của Chỉ số công việc điều dưỡng) và thái độ an toàn (Bảng câu hỏi thái độ an toàn). KẾT QUẢ Trong số 500 người tham gia đã đồng ý bằng văn bản, 474 (94,8%) đã điền vào bảng câu hỏi. Kết quả cho thấy việc sử dụng nhiều hơn các chiến lược đối phó theo hướng tiếp cận và ít hơn các chiến lược đối phó tránh né có liên quan đến sức khỏe tâm lý tốt hơn. Sức khỏe tâm lý có liên quan trực tiếp đến chất lượng môi trường thực hành và thái độ an toàn của y tá. Tác động của sức khỏe tâm lý đến thái độ an toàn được môi trường thực hành chất lượng trung gian đáng kể. Việc sử dụng các chiến lược đối phó theo hướng tiếp cận có khả năng dự đoán đáng kể sức khỏe tâm lý tích cực, môi trường thực hành tốt và thái độ an toàn tốt. KẾT LUẬN VÀ Ý NGHĨA LÂM SÀNG Nghiên cứu này tìm thấy một con đường riêng biệt cho mối quan hệ giữa sức khỏe tâm lý, môi trường thực hành và thái độ an toàn của y tá lâm sàng. Sức khỏe tâm lý ở y tá lâm sàng cao hơn đối với những người có nhiều chiến lược đối phó theo hướng tiếp cận hơn. Sức khỏe tâm lý ảnh hưởng trực tiếp đến thái độ an toàn, điều này ảnh hưởng đến môi trường thực hành của y tá. Những hàm ý thực tế của kết quả nghiên cứu cho thấy rằng các biện pháp can thiệp được thiết kế để thúc đẩy sức khỏe tâm lý tích cực có thể giúp cải thiện môi trường thực hành của y tá, từ đó có thể dẫn đến thái độ an toàn và kết quả chăm sóc điều dưỡng tốt hơn."} +{"idx": 1890098, "en": "The development of synthetic biology calls for accurate understanding of the critical functions that allow construction and operation of a living cell. Besides coding for ubiquitous structures, minimal genomes encode a wealth of functions that dissipate energy in an unanticipated way. Analysis of these functions shows that they are meant to manage information under conditions when discrimination of substrates in a noisy background is preferred over a simple recognition process. We show here that many of these functions, including transporters and the ribosome construction machinery, behave as would behave a material implementation of the information-managing agent theorized by Maxwell almost 150 years ago and commonly known as Maxwell's demon (MxD). A core gene set encoding these functions belongs to the minimal genome required to allow the construction of an autonomous cell. These MxDs allow the cell to perform computations in an energy-efficient way that is vastly better than our contemporary computers.", "gemini": "Sự phát triển của sinh học tổng hợp đòi hỏi sự hiểu biết chính xác về các chức năng quan trọng cho phép xây dựng và vận hành một tế bào sống. Bên cạnh việc mã hóa cho các cấu trúc phổ biến, các bộ gen tối thiểu mã hóa rất nhiều chức năng tiêu tan năng lượng theo một cách không lường trước. Phân tích các chức năng này cho thấy chúng có nghĩa là để quản lý thông tin trong điều kiện phân biệt các chất nền trong bối cảnh nhiễu được ưu tiên hơn một quá trình nhận dạng đơn giản. Chúng tôi trình bày ở đây rằng nhiều chức năng trong số này, bao gồm các chất vận chuyển và bộ máy xây dựng ribosome, hoạt động giống như một vật liệu thực hiện tác nhân quản lý thông tin được Maxwell đưa ra lý thuyết cách đây gần 150 năm và thường được gọi là ác quỷ Maxwell (MxD). Một bộ gen cốt lõi mã hóa các chức năng này thuộc về bộ gen tối thiểu cần thiết để cho phép xây dựng một tế bào tự chủ. Các MxD này cho phép tế bào thực hiện các phép tính theo cách tiết kiệm năng lượng tốt hơn nhiều so với các máy tính đương đại của chúng ta."} +{"idx": 1890099, "en": "Over the last 20 years, Italy has experienced multiple incursions of different serotypes of Bluetongue virus (BTV), a Culicoides-borne arbovirus, the causative agent of bluetongue (BT), a major disease of ruminants. The majority of these incursions originated from Northern Africa, likely because of wind-blown dissemination of infected midges. Here, we report the first identification of BTV-3 in Sardinia, Italy. BTV-3 circulation was evidenced in sentinel animals located in the province of Sud Sardegna on September 19, 2018. Prototype strain BTV-3 SAR2018 was isolated on cell culture. BTV-3 SAR2018 sequence and partial sequences obtained by next-generation sequencing from nucleic acids purified from the isolate and blood samples, respectively, were demonstrated to be almost identical (99-100% of nucleotide identity) to BTV-3 TUN2016 identified in Tunisia in 2016 and 2017, a scenario already observed in past incursions of other BTV serotypes originating from Northern Africa.", "gemini": "Trong 20 năm qua, Ý đã trải qua nhiều lần xâm nhập của các huyết thanh khác nhau của vi-rút lưỡi xanh (BTV), một loại arbovirus do Culicoides lây truyền, tác nhân gây bệnh lưỡi xanh (BT), một căn bệnh chính ở động vật nhai lại. Phần lớn các lần xâm nhập này có nguồn gốc từ Bắc Phi, có thể là do muỗi bị nhiễm bệnh do gió thổi. Ở đây, chúng tôi báo cáo lần đầu tiên xác định được BTV-3 ở Sardinia, Ý. Lưu hành BTV-3 đã được chứng minh ở các động vật gác tại tỉnh Sud Sardegna vào ngày 19 tháng 9 năm 2018. Chủng nguyên mẫu BTV-3 SAR2018 đã được phân lập trên nuôi cấy tế bào. Trình tự BTV-3 SAR2018 và trình tự một phần thu được bằng giải trình tự thế hệ tiếp theo từ axit nucleic được tinh chế từ mẫu phân lập và mẫu máu tương ứng, đã được chứng minh là gần giống hệt nhau (99-100% bản sắc nucleotide) với BTV-3 TUN2016 được xác định ở Tunisia vào năm 2016 và 2017, một kịch bản đã được quan sát thấy trong các lần xâm nhập trước của các huyết thanh BTV khác có nguồn gốc từ Bắc Phi."} +{"idx": 1890100, "en": "AIMS The aim of this study is to assess the impact of establishing a specialized community personality disorder team on out of area placements, local hospital admissions and out of hours crisis contacts for service users with borderline personality disorder. METHOD This is a before-after interim evaluation of a new service. We tested, through a paired t-test, whether the intervention generated statistically significant differences over a range of measures of service usage, including out of area placements, local hospital admissions and out of hours crisis contacts. Data from 12 months after the intervention started were compared with the previous 12 months of routine care, to determine the effect on crisis contacts, days in hospital and those returning from/or sent out of area for care. Finally, we have assessed the likelihood to generate cost savings. RESULTS All service users in out of area placements were repatriated to live in the community locally (100%), and there was a statistically significant decrease in inpatient admissions (80%). This was counterbalanced by a smaller but statistically significant increase in out of hours community crisis contacts (30%), although these reduced over time. CONCLUSION Reorganizing local care pathways can lead to less out of area placements and less hospital admissions. © 2019 John Wiley & Sons, Ltd.", "gemini": "MỤC ĐÍCH Mục đích của nghiên cứu này là đánh giá tác động của việc thành lập một nhóm chuyên về rối loạn nhân cách cộng đồng đối với các vị trí sắp xếp ngoài khu vực, nhập viện tại bệnh viện địa phương và các cuộc liên hệ khủng hoảng ngoài giờ đối với những người sử dụng dịch vụ mắc chứng rối loạn nhân cách ranh giới. PHƯƠNG PHÁP Đây là một đánh giá tạm thời trước và sau của một dịch vụ mới. Chúng tôi đã kiểm tra, thông qua kiểm tra t ghép đôi, liệu sự can thiệp có tạo ra sự khác biệt có ý nghĩa thống kê trong một loạt các biện pháp sử dụng dịch vụ hay không, bao gồm các vị trí sắp xếp ngoài khu vực, nhập viện tại bệnh viện địa phương và các cuộc liên hệ khủng hoảng ngoài giờ. Dữ liệu từ 12 tháng sau khi can thiệp bắt đầu được so sánh với 12 tháng trước đó của việc chăm sóc thường xuyên, để xác định tác động đến các cuộc liên hệ khủng hoảng, số ngày nằm viện và những người trở về/hoặc được gửi ra khỏi khu vực để chăm sóc. Cuối cùng, chúng tôi đã đánh giá khả năng tạo ra tiết kiệm chi phí. KẾT QUẢ Tất cả những người sử dụng dịch vụ ở các vị trí sắp xếp ngoài khu vực đều được hồi hương để sống tại cộng đồng địa phương (100%) và có sự sụt giảm đáng kể về mặt thống kê trong số lần nhập viện (80%). Điều này được cân bằng bởi sự gia tăng nhỏ hơn nhưng có ý nghĩa thống kê về các cuộc liên hệ khủng hoảng cộng đồng ngoài giờ (30%), mặc dù những cuộc liên hệ này đã giảm theo thời gian. KẾT LUẬN Việc tổ chức lại các con đường chăm sóc tại địa phương có thể dẫn đến ít vị trí sắp xếp ngoài khu vực hơn và ít nhập viện hơn. © 2019 John Wiley & Sons, Ltd."} +{"idx": 1890101, "en": "OBJECTIVE Asthma exacerbations are common presentations to ED. Key guideline recommendations for management include administration of inhaled bronchodilators, systemic corticosteroids and titrated oxygen therapy. Our aim was to compare management and outcomes between patients treated for asthma in Europe (EUR) and South East Asia/Australasia (SEA) and compliance with international guidelines. METHODS In each region, prospective, interrupted time series studies were performed including adult (age >18 years) patients presenting to ED with the main complaint of dyspnoea during three 72 h periods. This was a planned sub-study that included those with an ED primary diagnosis of asthma. Data was collected on demographics, clinical features, treatment in ED, diagnosis, disposition and in-hospital outcome. The results of interest were differences in treatment and outcome between EUR and SEA cohorts. RESULTS Five hundred and eighty-four patients were identified from 112 EDs (66 EUR and 46 SEA). The cohorts had similar demographics and co-morbidity patterns, with 89% of the cohort having a previous diagnosis of asthma. There were no significant differences in treatment between EUR and SEA patients - inhaled beta-agonists were administered in 86% of cases, systemic corticosteroids in 66%, oxygen therapy in 44% and antibiotics in 20%. Two thirds of patients were discharged home from the ED. CONCLUSION The data suggests that compliance with guideline-recommended therapy in both regions, particularly corticosteroid administration, is sub-optimal. It also suggests over-use of antibiotics.", "gemini": "MỤC TIÊU Các đợt kịch phát hen suyễn là biểu hiện phổ biến ở khoa cấp cứu. Các khuyến nghị chính của hướng dẫn về quản lý bao gồm sử dụng thuốc giãn phế quản dạng hít, corticosteroid toàn thân và liệu pháp oxy chuẩn độ. Mục đích của chúng tôi là so sánh cách quản lý và kết quả giữa những bệnh nhân được điều trị hen suyễn ở Châu Âu (EUR) và Đông Nam Á/Úc (SEA) và tuân thủ các hướng dẫn quốc tế. PHƯƠNG PHÁP Ở mỗi khu vực, các nghiên cứu chuỗi thời gian ngắt quãng, có triển vọng được thực hiện bao gồm những bệnh nhân trưởng thành (tuổi> 18) đến khoa cấp cứu với khiếu nại chính là khó thở trong ba khoảng thời gian 72 giờ. Đây là một nghiên cứu phụ được lập kế hoạch bao gồm những người được chẩn đoán chính tại khoa cấp cứu là hen suyễn. Dữ liệu được thu thập về thông tin nhân khẩu học, các đặc điểm lâm sàng, cách điều trị tại khoa cấp cứu, chẩn đoán, tình trạng và kết quả trong bệnh viện. Kết quả quan tâm là sự khác biệt về cách điều trị và kết quả giữa các nhóm EUR và SEA. KẾT QUẢ Năm trăm tám mươi bốn bệnh nhân đã được xác định từ 112 khoa cấp cứu (66 EUR và 46 SEA). Các nhóm có thông tin nhân khẩu học và các kiểu bệnh đi kèm tương tự nhau, với 89% nhóm có chẩn đoán hen suyễn trước đó. Không có sự khác biệt đáng kể nào trong cách điều trị giữa bệnh nhân EUR và SEA - thuốc chủ vận beta dạng hít được sử dụng trong 86% các trường hợp, corticosteroid toàn thân trong 66%, liệu pháp oxy trong 44% và kháng sinh trong 20%. Hai phần ba bệnh nhân được xuất viện về nhà từ khoa cấp cứu. KẾT LUẬN Dữ liệu cho thấy việc tuân thủ liệu pháp được khuyến cáo theo hướng dẫn ở cả hai khu vực, đặc biệt là dùng corticosteroid, là chưa tối ưu. Nó cũng cho thấy việc sử dụng kháng sinh quá mức."} +{"idx": 1890102, "en": "A comprehensive characterization of lung adenocarcinoma (LADC) clinical features is currently missing. We prospectively evaluated Caucasian patients with early-stage LADC. Patients with LADC diagnosed between 2011 and 2015 were prospectively assessed for lung resection with curative intent. Fifty clinical, pathologic, radiologic, and molecular variables were recorded. Patients were followed till death/study conclusion. The main findings were compared to a separate cohort from France. Of 1943 patients evaluated, 366 were enrolled (18.8%; 181 female; 75 never-smokers; 28% of registered Bavarian cases over the study period). Smoking and obstruction were significantly more prevalent in GLAD compared with adult Bavarians (P < 0.0001). Ever-smoker tumors were preferentially localized to the upper lobes. We observed 120 relapses and 74 deaths over 704 cumulative follow-up years. Median overall and disease-free survival were >7.5 and 3.6 years, respectively. Patients aged <45 or >65 years, resected >60 days postdiagnosis, with abnormal FVC/DLCO VA , N2/N3 stage, or solid histology had significantly decreased survival estimates. These were fit into a weighted locoregional LADC death risk score that outperformed pTNM7 in predicting survival in the GLAD and in our second cohort. We define the clinical gestalt of locoregional LADC and provide a new clinical tool to predict survival, findings that may aid future management and research design.", "gemini": "Hiện vẫn chưa có đặc điểm lâm sàng đầy đủ của ung thư biểu mô phổi tuyến (LADC). Chúng tôi đã đánh giá triển vọng những bệnh nhân da trắng mắc LADC giai đoạn đầu. Những bệnh nhân được chẩn đoán mắc LADC từ năm 2011 đến năm 2015 đã được đánh giá triển vọng để phẫu thuật cắt phổi với mục đích điều trị khỏi. Có 50 biến số lâm sàng, bệnh lý, X quang và phân tử đã được ghi lại. Bệnh nhân được theo dõi cho đến khi tử vong/kết thúc nghiên cứu. Những phát hiện chính đã được so sánh với một nhóm riêng biệt từ Pháp. Trong số 1943 bệnh nhân được đánh giá, 366 bệnh nhân đã được ghi danh (18,8%; 181 nữ; 75 người không bao giờ hút thuốc; 28% các trường hợp người Bavaria đã đăng ký trong suốt thời gian nghiên cứu). Hút thuốc và tắc nghẽn phổ biến hơn đáng kể ở GLAD so với người Bavaria trưởng thành (P < 0,0001). Các khối u của người đã từng hút thuốc thường tập trung ở thùy trên. Chúng tôi đã quan sát thấy 120 lần tái phát và 74 trường hợp tử vong trong hơn 704 năm theo dõi tích lũy. Tuổi thọ trung bình chung và không có bệnh lần lượt là > 7,5 và 3,6 năm. Những bệnh nhân dưới 45 tuổi hoặc trên 65 tuổi, cắt bỏ > 60 ngày sau chẩn đoán, có FVC/DLCO VA bất thường, giai đoạn N2/N3 hoặc mô học dạng đặc có tỷ lệ sống sót giảm đáng kể. Những điều này phù hợp với điểm rủi ro tử vong do LADC tại chỗ trong khu vực được cân nhắc, cao hơn pTNM7 trong việc dự đoán khả năng sống sót ở GLAD và trong nhóm thứ hai của chúng tôi. Chúng tôi xác định hình thái lâm sàng của LADC tại chỗ trong khu vực và cung cấp một công cụ lâm sàng mới để dự đoán khả năng sống sót, những phát hiện có thể hỗ trợ cho việc quản lý và thiết kế nghiên cứu trong tương lai."} +{"idx": 1890103, "en": "Breast cancer is one of the most common malignant tumors among women worldwide. About 70-75% of primary breast cancers belong to estrogen receptor (ER)-positive breast cancer. In the development of ER-positive breast cancer, abnormal activation of the ERα pathway plays an important role and is also a key point leading to the failure of clinical endocrine therapy. In this study, we found that the small molecule peptide chlorotoxin (CTX) can significantly inhibit the proliferation, migration and invasion of breast cancer cells. In in vitro study, CTX inhibits the expression of ERα in breast cancer cells. Further studies showed that CTX can directly bind to ERα and change the protein secondary structure of its LBD domain, thereby inhibiting the ERα signaling pathway. In addition, we also found that vasodilator stimulated phosphoprotein (VASP) is a target gene of ERα signaling pathway, and CTX can inhibit breast cancer cell proliferation, migration, and invasion through ERα/VASP signaling pathway. In in vivo study, CTX significantly inhibits growth of ER overexpressing breast tumor and, more importantly, based on the mechanism of CTX interacting with ERα, we found that CTX can target ER overexpressing breast tumors in vivo. Our study reveals a new mechanism of CTX anti-ER-positive breast cancer, which also provides an important reference for the study of CTX anti-ER-related tumors.", "gemini": "Ung thư vú là một trong những khối u ác tính phổ biến nhất ở phụ nữ trên toàn thế giới. Khoảng 70-75% ung thư vú nguyên phát thuộc về ung thư vú dương tính với thụ thể estrogen (ER). Trong quá trình phát triển của ung thư vú dương tính với ER, sự hoạt hóa bất thường của con đường ERα đóng vai trò quan trọng và cũng là điểm then chốt dẫn đến thất bại của liệu pháp nội tiết lâm sàng. Trong nghiên cứu này, chúng tôi phát hiện ra rằng peptide phân tử nhỏ chlorotoxin (CTX) có thể ức chế đáng kể sự tăng sinh, di chuyển và xâm lấn của các tế bào ung thư vú. Trong nghiên cứu in vitro, CTX ức chế sự biểu hiện của ERα trong các tế bào ung thư vú. Các nghiên cứu sâu hơn cho thấy CTX có thể liên kết trực tiếp với ERα và thay đổi cấu trúc thứ cấp của protein của miền LBD, do đó ức chế con đường tín hiệu ERα. Ngoài ra, chúng tôi cũng phát hiện ra rằng protein kích thích mạch máu (VASP) là một gen mục tiêu của con đường tín hiệu ERα và CTX có thể ức chế sự tăng sinh, di chuyển và xâm lấn của tế bào ung thư vú thông qua con đường tín hiệu ERα/VASP. Trong nghiên cứu in vivo, CTX ức chế đáng kể sự phát triển của khối u vú biểu hiện quá mức ER và quan trọng hơn, dựa trên cơ chế CTX tương tác với ERα, chúng tôi phát hiện ra rằng CTX có thể nhắm vào các khối u vú biểu hiện quá mức ER in vivo. Nghiên cứu của chúng tôi tiết lộ một cơ chế mới về CTX chống lại ung thư vú dương tính với ER, đồng thời cũng cung cấp một tài liệu tham khảo quan trọng cho việc nghiên cứu các khối u liên quan đến CTX chống ER."} +{"idx": 1890104, "en": "The 21st century is regarded as the century of biotechnological drugs, among which monoclonal antibodies and their derived targeting drugs have established themselves as the leading modality of biopharmaceutical pharmaceutics for a wide range of indications covering malignant tumors and autoimmune disorders. Since the manufacturing of the first antibody drug from hybridoma cells, the technologies have been intensely studied and there emerged numerous breakthroughs in recombinant cell line establishment, antibody expression and purification, quality control and other related areas. This article summarizes the critical progresses of antibody drugs expression technologies, especially of mammalian cell expression system, Escherichia coli expression system, the transgenic animal reactor and the cell free protein synthesis system, to give a detailed illustration of the recent advances in antibody drugs development.", "gemini": "Thế kỷ 21 được coi là thế kỷ của các loại thuốc công nghệ sinh học, trong đó các kháng thể đơn dòng và các loại thuốc nhắm mục tiêu có nguồn gốc từ chúng đã trở thành phương thức hàng đầu của dược phẩm sinh học cho nhiều chỉ định bao gồm khối u ác tính và các rối loạn tự miễn dịch. Kể từ khi sản xuất loại thuốc kháng thể đầu tiên từ tế bào lai ghép, các công nghệ đã được nghiên cứu sâu rộng và đã xuất hiện nhiều đột phá trong việc thiết lập dòng tế bào tái tổ hợp, biểu hiện và tinh chế kháng thể, kiểm soát chất lượng và các lĩnh vực liên quan khác. Bài viết này tóm tắt những tiến bộ quan trọng của các công nghệ biểu hiện thuốc kháng thể, đặc biệt là hệ thống biểu hiện tế bào động vật có vú, hệ thống biểu hiện Escherichia coli, lò phản ứng động vật chuyển gen và hệ thống tổng hợp protein không có tế bào, để minh họa chi tiết về những tiến bộ gần đây trong phát triển thuốc kháng thể."} +{"idx": 1890105, "en": "Monoclonal antibodies have become the main type of antibody drug because of their high specificity and strong affinity to antigen. However, with the intensive study of the natural monoclonal antibody, many defects have faced, such as the limit times of binding to antigen, the unanticipated antibody clearance and antigen accumulation. Therefore, studies are no longer limited to the natural antibody screening, but rather to improve the efficiency of antibody drugs by engineering. In recent years, the bottlenecks in the development of conventional antibody have been solved effectively since the discovery of a novel recycling antibody. Recycling antibody binds to an antigen in plasma and dissociates from the antigen in endosome, thus maximizing the use of antibody and reducing antigen-mediated antibody clearance and antibody-mediated antigen accumulation. In addition, recycling antibodies can enhance the affinity with Fc receptors through further Fc modification. This paper reviews the research progress of circulating antibodies, including its characteristics, transformation methods and prospects.", "gemini": "Kháng thể đơn dòng đã trở thành loại thuốc kháng thể chính nhờ tính đặc hiệu cao và ái lực mạnh với kháng nguyên. Tuy nhiên, với nghiên cứu chuyên sâu về kháng thể đơn dòng tự nhiên, nhiều khuyết điểm đã phải đối mặt, chẳng hạn như thời gian liên kết với kháng nguyên, thanh thải kháng thể không mong muốn và tích tụ kháng nguyên. Do đó, các nghiên cứu không còn giới hạn ở việc sàng lọc kháng thể tự nhiên, mà là cải thiện hiệu quả của thuốc kháng thể bằng kỹ thuật. Trong những năm gần đây, nút thắt cổ chai trong phát triển kháng thể thông thường đã được giải quyết hiệu quả kể từ khi phát hiện ra một kháng thể tái chế mới. Kháng thể tái chế liên kết với kháng nguyên trong huyết tương và tách khỏi kháng nguyên trong nội thể, do đó tối đa hóa việc sử dụng kháng thể và giảm thanh thải kháng thể do kháng nguyên và tích tụ kháng nguyên do kháng thể. Ngoài ra, kháng thể tái chế có thể tăng cường ái lực với thụ thể Fc thông qua sửa đổi Fc thêm. Bài báo này tổng quan về tiến trình nghiên cứu kháng thể tuần hoàn, bao gồm đặc điểm, phương pháp chuyển đổi và triển vọng của nó."} +{"idx": 1890106, "en": "Environmental protection and energy supply are our two major concerns. Greenhouse gases released from energy consumption have serious impact on the environment. CO₂ fixation can be used to convert CO₂ into fuels or chemicals. However, natural carbon-fixing organisms usually have some disadvantages such as slow growth and low carbon fixation efficiency. Enhancing or remodeling CO₂ fixation pathways in model microorganisms can realize CO₂ recycling, which can further increase fuel or chemical production and reduce greenhouse gas emission. This review describes in detail metabolic engineering of CO₂ fixation pathways to improve chemical production and sugar synthesis, elaborates the role of relevant metabolic pathways and key enzymes in CO₂ fixation, introduces the application of electro-biochemical synthesis system, shows the great potential of CO₂ fixation, and prospects the future research direction of CO₂ fixation.", "gemini": "Bảo vệ môi trường và cung cấp năng lượng là hai mối quan tâm chính của chúng ta. Khí nhà kính thải ra từ việc tiêu thụ năng lượng có tác động nghiêm trọng đến môi trường. Có thể sử dụng cố định CO₂ để chuyển đổi CO₂ thành nhiên liệu hoặc hóa chất. Tuy nhiên, các sinh vật cố định cacbon tự nhiên thường có một số nhược điểm như tăng trưởng chậm và hiệu quả cố định cacbon thấp. Việc tăng cường hoặc cải tạo các con đường cố định CO₂ trong các vi sinh vật mô hình có thể thực hiện tái chế CO₂, có thể làm tăng thêm sản xuất nhiên liệu hoặc hóa chất và giảm phát thải khí nhà kính. Bài đánh giá này mô tả chi tiết về kỹ thuật chuyển hóa các con đường cố định CO₂ để cải thiện sản xuất hóa chất và tổng hợp đường, giải thích vai trò của các con đường chuyển hóa có liên quan và các enzym chính trong quá trình cố định CO₂, giới thiệu ứng dụng của hệ thống tổng hợp điện sinh học, cho thấy tiềm năng lớn của quá trình cố định CO₂ và triển vọng hướng nghiên cứu trong tương lai về quá trình cố định CO₂."} +{"idx": 1890107, "en": "Urease decomposes urea to ammonia, and has application potential in agriculture and medical treatment. Urease proteins include structural proteins (UreA, UreB and UreC) and accessory proteins (UreD/UreH, UreE, UreF and UreG), each of them has its own unique role in urease maturation. The structural proteins form the active center of urease, and the accessory proteins are responsible for the delivery of nickel. We review here the structure and function of bacterial urease complexes, and how each protein interacts to complete the activation process. We hope to provide theoretical basis for the regulation of urease activity and the development of urease inhibitors.", "gemini": "Urease phân hủy urê thành amoniac, và có tiềm năng ứng dụng trong nông nghiệp và điều trị y tế. Protein urease bao gồm protein cấu trúc (UreA, UreB và UreC) và protein phụ trợ (UreD/UreH, UreE, UreF và UreG), mỗi loại có vai trò riêng trong quá trình trưởng thành của urease. Các protein cấu trúc tạo thành trung tâm hoạt động của urease, và các protein phụ trợ chịu trách nhiệm cung cấp niken. Ở đây chúng tôi xem xét cấu trúc và chức năng của các phức hợp urease của vi khuẩn, và cách thức tương tác của từng protein để hoàn thành quá trình hoạt hóa. Chúng tôi hy vọng sẽ cung cấp cơ sở lý thuyết cho việc điều chỉnh hoạt động của urease và phát triển các chất ức chế urease."} +{"idx": 1890108, "en": "Nineteen topics were selected as major clinical research advances in gynecologic oncology in 2018. For cervical cancer, the importance of human papillomavirus (HPV) testing alone as primary cervical cancer screening method and negative survival impact of minimally invasive surgery in early-stage cervical cancer were addressed. For ovarian cancer, cost-effectiveness of genetic testing to prevent cancer, use of analgesics and oral pill to reduce cancer risk, efficacy of secondary cytoreductive surgery and hyperthermic intraperitoneal chemotherapy, update in the use of poly (ADP-ribose) polymerase inhibitor, and efficacy of anti-angiogenic targeted treatments, including bevacizumab and tyrosine kinase inhibitors, were reviewed. For corpus cancer, sentinel lymph node mapping technique, adjuvant therapy in high-risk endometrial cancer (PORTEC-3), and targeted therapy in recurrent disease were covered. For the field of radiation oncology, survival outcomes of chemoradiation compared with chemotherapy alone in metastatic cervical cancer and new findings regarding the use of neoadjuvant chemotherapy in locally advanced cervical cancer were introduced. Lastly, for breast cancer, the use of talazoparib in patients with germline BRCA1/2 mutation, ovarian suppression for premenopausal patients, adjuvant chemotherapy guided by 21-gene assay, and combination therapy of atezolizumab and nab-paclitaxel for triple-negative cancer as well as promising overall survival results of palbociclib and fulvestrant in advanced breast cancer were briefly mentioned.", "gemini": "Mười chín chủ đề được chọn là những tiến bộ nghiên cứu lâm sàng chính trong ung thư phụ khoa vào năm 2018. Đối với ung thư cổ tử cung, tầm quan trọng của xét nghiệm vi-rút papilloma ở người (HPV) đơn lẻ như phương pháp sàng lọc ung thư cổ tử cung chính và tác động tiêu cực đến sự sống còn của phẫu thuật ít xâm lấn ở giai đoạn đầu ung thư cổ tử cung đã được giải quyết. Đối với ung thư buồng trứng, hiệu quả về chi phí của xét nghiệm di truyền để phòng ngừa ung thư, sử dụng thuốc giảm đau và thuốc uống để giảm nguy cơ ung thư, hiệu quả của phẫu thuật cắt bỏ thứ phát và hóa trị nội phúc mạc tăng nhiệt, cập nhật việc sử dụng chất ức chế poly (ADP-ribose) polymerase, và hiệu quả của các phương pháp điều trị nhắm mục tiêu chống hình thành mạch máu, bao gồm bevacizumab và chất ức chế tyrosine kinase, đã được xem xét. Đối với ung thư thân tử cung, kỹ thuật lập bản đồ hạch bạch huyết canh, liệu pháp bổ trợ trong ung thư nội mạc tử cung có nguy cơ cao (PORTEC-3) và liệu pháp nhắm mục tiêu trong bệnh tái phát đã được đề cập. Đối với lĩnh vực xạ trị ung thư, kết quả sống sót của hóa xạ trị so với hóa trị đơn thuần trong ung thư cổ tử cung di căn và những phát hiện mới về việc sử dụng hóa trị tân bổ trợ trong ung thư cổ tử cung tiến triển tại chỗ đã được giới thiệu. Cuối cùng, đối với ung thư vú, việc sử dụng talazoparib ở những bệnh nhân có đột biến BRCA1/2 ở dòng mầm, ức chế buồng trứng cho bệnh nhân tiền mãn kinh, hóa trị bổ trợ được hướng dẫn bởi xét nghiệm 21 gen và liệu pháp kết hợp atezolizumab và nab-paclitaxel cho ung thư ba âm tính cũng như kết quả sống sót chung đầy hứa hẹn của palbociclib và fulvestrant trong ung thư vú tiến triển đã được đề cập ngắn gọn."} +{"idx": 1890109, "en": "The mucosae represent the first line of defense against the invasion of most pathogens, and the mucosal immune system plays a crucial role in the control of infection. Mucosal vaccination can trigger both humoral and cell-mediated immune responses mucosally as well as systemically. Hence, protective immune responses can be elicited effectively by mucosal vaccination. Microfold (M) cells being unique to the mucosal immune system can take up luminal antigens and initiating antigen-specific immune responses. The number of antigen uptake by M cells is directly related to the immune efficacy of mucosal vaccines. Utilizing M cell ligands, M cells-targeting antigen delivery can achieve highly effective mucosal immune responses. The strategy of targeted delivery of antigens to M cells and its applications can be used for the improvement of mucosal immune responses and the development of mucosal vaccines. Despite these efforts, successful development of safe and effective mucosal vaccines remains a big challenge and needs a long way to go, and provably still resort to further researches on cellular properties and functions as well as mucosal immune mechanisms.", "gemini": "Niêm mạc đại diện cho tuyến phòng thủ đầu tiên chống lại sự xâm nhập của hầu hết các tác nhân gây bệnh và hệ thống miễn dịch niêm mạc đóng vai trò quan trọng trong việc kiểm soát nhiễm trùng. Vắc-xin niêm mạc có thể kích hoạt cả phản ứng miễn dịch dịch thể và tế bào qua trung gian ở niêm mạc cũng như toàn thân. Do đó, phản ứng miễn dịch bảo vệ có thể được tạo ra hiệu quả bằng vắc-xin niêm mạc. Tế bào vi nếp (M) là duy nhất đối với hệ thống miễn dịch niêm mạc có thể lấy các kháng nguyên trong lòng ống và khởi động phản ứng miễn dịch đặc hiệu với kháng nguyên. Số lượng kháng nguyên được tế bào M hấp thụ liên quan trực tiếp đến hiệu quả miễn dịch của vắc-xin niêm mạc. Sử dụng các phối tử tế bào M, cung cấp kháng nguyên nhắm mục tiêu tế bào M có thể đạt được phản ứng miễn dịch niêm mạc có hiệu quả cao. Chiến lược cung cấp kháng nguyên có mục tiêu đến tế bào M và các ứng dụng của nó có thể được sử dụng để cải thiện phản ứng miễn dịch niêm mạc và phát triển vắc-xin niêm mạc. Mặc dù có những nỗ lực này, việc phát triển thành công vắc-xin niêm mạc an toàn và hiệu quả vẫn là một thách thức lớn và cần phải đi một chặng đường dài, và có thể vẫn phải tiếp tục nghiên cứu sâu hơn về các đặc tính và chức năng của tế bào cũng như cơ chế miễn dịch niêm mạc."} +{"idx": 1890110, "en": "Pitch deposits have negative effects on product quality, machine performance and production line profitability during pulp and paper manufacture. As traditional pitch control technology cannot provide satisfactory solutions in the pitch deposits, the enzymatic treatment has been rapidly developed for its high efficiency and pollution-free property. In this review, the chemical composition and present form of the pitch in pulp is first introduced, followed by a description of the pitch control enzymes. The emphasis is on the current research on enzymatic solutions to pitch problems, including the reaction mechanism, technology, and the present main problems of lipase, sterol esterases, laccase and lipoxygenase. Finally, the technology prospects in this field are proposed.", "gemini": "Cặn nhựa có tác động tiêu cực đến chất lượng sản phẩm, hiệu suất máy móc và lợi nhuận của dây chuyền sản xuất trong quá trình sản xuất bột giấy và giấy. Vì công nghệ kiểm soát cặn nhựa truyền thống không thể cung cấp các giải pháp thỏa đáng trong việc lắng cặn nhựa, nên việc xử lý bằng enzym đã được phát triển nhanh chóng vì hiệu quả cao và tính chất không gây ô nhiễm. Trong bài tổng quan này, đầu tiên giới thiệu thành phần hóa học và dạng hiện tại của cặn nhựa trong bột giấy, sau đó mô tả các enzym kiểm soát cặn nhựa. Trọng tâm là nghiên cứu hiện tại về các giải pháp enzym cho các vấn đề về cặn nhựa, bao gồm cơ chế phản ứng, công nghệ và các vấn đề chính hiện nay của lipase, sterol estease, laccase và lipoxygenase. Cuối cùng, triển vọng công nghệ trong lĩnh vực này được đề xuất."} +{"idx": 1890111, "en": "The study aims to use CRISPR/Cas9 introducing foreign gene targeted knock-in into chicken EAV-HP genome. First, specific primers were designed for amplification of EAV-HP left, right homologous arms and enhanced green fluorescent protein (eGFP) expression cassette. PCR products of homologous arms were ligated to both sides of eGFP by overlap extension PCR, resulting in full-length donor DNA fragment designated as LER. Then LER fragments were cloned into pMD19-T to obtain donor vector pMDT-LER. Subsequently, the donor vector pMDT-LER was transfected into HEK293T cells to verify the expression of eGFP gene. Furthermore, co-transfection of CRISPR/Cas9 expression vector and pMDT-LER into chicken DF-1 cells was performed to achieve eGFP transgenic cells. Meanwhile, eGFP expression was observed in cells, and the event of eGFP integration into EAV-HP genome was detectable by amplification of target DNA. Finally, the transgenic DF-1 cells were passaged seven times, and the stable integration and expression of eGFP was checked by PCR and Western blotting. These results demonstrated that eGFP gene was knocked into the EAV-HP genome successfully, which provides a new integration site for research of transgenic chicken.", "gemini": "Nghiên cứu này nhằm sử dụng CRISPR/Cas9 đưa gen ngoại lai nhắm mục tiêu vào bộ gen EAV-HP của gà. Đầu tiên, các đoạn mồi đặc hiệu được thiết kế để khuếch đại cánh tay trái, phải đồng đẳng của EAV-HP và hộp biểu hiện protein huỳnh quang xanh tăng cường (eGFP). Các sản phẩm PCR của cánh tay đồng đẳng được gắn vào cả hai bên của eGFP bằng PCR mở rộng chồng chéo, tạo ra đoạn DNA của gen cho toàn bộ chiều dài được chỉ định là LER. Sau đó, các đoạn LER được nhân bản vào pMD19-T để thu được vectơ cho gen pMDT-LER. Tiếp theo, vectơ cho gen pMDT-LER được chuyển vào tế bào HEK293T để xác minh biểu hiện của gen eGFP. Hơn nữa, quá trình chuyển gen đồng thời của vectơ biểu hiện CRISPR/Cas9 và pMDT-LER vào tế bào DF-1 của gà đã được thực hiện để đạt được các tế bào chuyển gen eGFP. Trong khi đó, biểu hiện eGFP được quan sát thấy trong các tế bào và sự kiện tích hợp eGFP vào bộ gen EAV-HP có thể phát hiện được bằng cách khuếch đại DNA mục tiêu. Cuối cùng, các tế bào DF-1 chuyển gen được nuôi cấy bảy lần và sự tích hợp và biểu hiện ổn định của eGFP đã được kiểm tra bằng PCR và phương pháp Western blotting. Những kết quả này chứng minh rằng gen eGFP đã được đưa vào bộ gen EAV-HP thành công, tạo ra một vị trí tích hợp mới để nghiên cứu gà chuyển gen."} +{"idx": 1890112, "en": "Natural lignocellulosic materials contain cellulose, hemicellulose, and lignin. Cellulose hydrolysis to glucose requires a series of lignocellulases. Recently, the research on the synergistic effect of lignocellulases has become a new research focus. Here, four lignocellulase genes encoding β-glucosidase, endo-1,4-β-glucanase, xylanase and laccase from termite and their endosymbionts were cloned into pETDuet-1 and pRSFDuet-1 and expressed in Escherichia coli. After SDS-PAGE analysis, the corresponding protein bands consistent with the theoretical values were observed and all the proteins showed enzyme activities. We used phosphoric acid swollen cellulose (PASC) as substrate to measure the synergistic effect of crude extracts of co-expressing enzymes and the mixture of single enzyme. The co-expressed enzymes increased the degradation efficiency of PASC by 44% compared with the single enzyme mixture; while the degradation rate increased by 34% and 20%, respectively when using filter paper and corn cob pretreated with phosphoric acid as substrates. The degradation efficiency of the co-expressed enzymes was higher than the total efficiency of the single enzyme mixture.", "gemini": "Các vật liệu lignocellulose tự nhiên chứa cellulose, hemicellulose và lignin. Quá trình thủy phân cellulose thành glucose đòi hỏi một loạt các lignocellulase. Gần đây, nghiên cứu về hiệu ứng hiệp đồng của lignocellulase đã trở thành một trọng tâm nghiên cứu mới. Ở đây, bốn gen lignocellulase mã hóa β-glucosidase, endo-1,4-β-glucanase, xylanase và laccase từ mối và cộng sinh nội bào của chúng đã được nhân bản vào pETDuet-1 và pRSFDuet-1 và được biểu hiện trong Escherichia coli. Sau khi phân tích SDS-PAGE, các dải protein tương ứng phù hợp với các giá trị lý thuyết đã được quan sát thấy và tất cả các protein đều cho thấy hoạt tính của enzyme. Chúng tôi đã sử dụng cellulose phồng axit photphoric (PASC) làm chất nền để đo hiệu ứng hiệp đồng của các chiết xuất thô của các enzyme biểu hiện đồng thời và hỗn hợp của enzyme đơn lẻ. Các enzyme biểu hiện đồng thời làm tăng hiệu quả phân hủy của PASC lên 44% so với hỗn hợp enzyme đơn lẻ; trong khi tốc độ phân hủy tăng lần lượt 34% và 20% khi sử dụng giấy lọc và lõi ngô được xử lý trước bằng axit photphoric làm chất nền. Hiệu quả phân hủy của các enzyme biểu hiện đồng thời cao hơn hiệu quả tổng thể của hỗn hợp enzyme đơn lẻ."} +{"idx": 1890113, "en": "Escherichia coli was metabolically engineered to produce poly(glycolate-co-lactate-co-3-hydroxybutyrate) using glucose and xylose as carbon sources. The combinatorial biosynthetic route was constructed by the overexpression of a series of enzymes including D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase, and polyhydroxyalkanoate synthase. Overexpression of polyhydroxyalkanoate granule associated protein significantly improved biopolymer synthesis, and the recombinant strain reached 3.73 g/L cell dry weight with 38.72% (W/W) biopolymer content. A co-culture engineering strategy was developed to produce biopolymer from a mixture of glucose and xylose, achieving 4.01 g/L cell dry weight containing 21.54% (W/W) biopolymer. The results of this work offer an approach for simultaneously utilizing glucose and xylose and indicate the potential for future biopolymer production from lignocellulosic biomass.", "gemini": "Escherichia coli đã được thiết kế chuyển hóa để sản xuất poly(glycolate-co-lactate-co-3-hydroxybutyrate) sử dụng glucose và xylose làm nguồn cacbon. Con đường tổng hợp kết hợp được xây dựng bằng cách biểu hiện quá mức một loạt các enzyme bao gồm D-tagatose 3-epimerase, L-fuculokinase, L-fuculose-phosphate aldolase, aldehyde dehydrogenase, propionyl-CoA transferase, β-ketothiolase, acetoacetyl-CoA reductase và polyhydroxyalkanoate synthase. Biểu hiện quá mức của protein liên kết với hạt polyhydroxyalkanoate cải thiện đáng kể quá trình tổng hợp biopolymer và chủng tái tổ hợp đạt 3,73 g/L trọng lượng khô tế bào với hàm lượng biopolymer 38,72% (W/W). Một chiến lược kỹ thuật nuôi cấy đồng thời đã được phát triển để sản xuất biopolymer từ hỗn hợp glucose và xylose, đạt 4,01 g/L trọng lượng khô tế bào có chứa 21,54% (W/W) biopolymer. Kết quả của công trình này đưa ra một phương pháp tiếp cận để đồng thời sử dụng glucose và xylose và chỉ ra tiềm năng sản xuất biopolymer trong tương lai từ sinh khối lignocellulose."} +{"idx": 1890114, "en": "DNA methylation is an important type of epigenetic modification in eukaryotes. In order to research genome-wide methylation levels and patterns in foxtail millet (Setaria italica), the Methylation Sensitive Amplified Polymorphism (MSAP) analysis (employing double digestion with EcoR I and Hpa II/Msp I) was established and applied in two foxtail millet cultivars (Chaogu 58 and Yugu 1). The results showed that 32 pairs of MSAP primers were selected from 100 MSAP primers, and 1 615 and 1 482 clearly distinguishable and reproducible bands were amplified from Chaogu 58 and Yugu 1 respectively, including 3 types of methylation patterns. Cytosine methylation levels of CCGG context in Chaogu 58 and Yugu 1 were characterized as 6.93% and 8.77% respectively. Such different genomic DNA methylation levels between two foxtail millet varieties may provide a preliminary reference for the cultivation of this crop from a novel epigenetic viewpoint.", "gemini": "Methyl hóa DNA là một loại sửa đổi biểu sinh quan trọng ở eukaryote. Để nghiên cứu mức độ và kiểu methyl hóa toàn bộ hệ gen ở kê đuôi chồn (Setaria italica), phân tích Đa hình khuếch đại nhạy với methyl hóa (MSAP) (sử dụng tiêu hóa kép với EcoR I và Hpa II/Msp I) đã được thiết lập và áp dụng ở hai giống kê đuôi chồn (Chaogu 58 và Yugu 1). Kết quả cho thấy 32 cặp mồi MSAP được chọn từ 100 mồi MSAP, và 1.615 và 1.482 dải dễ phân biệt và tái tạo được đã được khuếch đại từ Chaogu 58 và Yugu 1 tương ứng, bao gồm 3 loại kiểu methyl hóa. Mức độ methyl hóa cytosine của ngữ cảnh CCGG ở Chaogu 58 và Yugu 1 được đặc trưng là 6,93% và 8,77% tương ứng. Mức độ methyl hóa DNA bộ gen khác nhau như vậy giữa hai giống kê đuôi chồn có thể cung cấp một tài liệu tham khảo sơ bộ cho việc canh tác cây trồng này từ quan điểm biểu sinh mới."} +{"idx": 1890115, "en": "Creating new germplasms and breeding new cultivars in peanut by radiation mutagenesis and tissue culture were conducted in this study, aiming to develop new breeding method of peanut. Mature seeds from Luhua 11, the most commonly grown peanut cultivar in Northern China, were treated by fast neutron irradiation. Then the embryo leaflets were separated from the irradiated seeds and inoculated on the media, and the regenerated seedlings were obtained through somatic embryogenesis pathway. The regenerated seedlings were grafted, acclimated and then transplanted into field and the selfed pods were harvested from 83 regenerated plants. The progenies were selected by the pedigree method, and 107 mutants were obtained from the progenies of the 83 regenerated plants. Different mutants showed obvious variation in many agronomic traits, including main stem height, branch number, pod shape and size, seed coat color, inner seed coat color, oil content and protein content etc. Yuhua 7, a new peanut variety with low oil content, early maturity and waterlogging tolerance was obtained. The yield of Yuhua 7 was over 14% higher than that of the mutagenic parent Luhua 11, and the oil content of kernels was 47.0%, lower than that of parent Luhua 11 with 52.1% oil content. Yuhua 7 had passed peanut variety regional multi-location trials in Liaoning Province in 2016 and its average yield was 13.8% higher than that of the control variety Baisha 1017. It had also passed national peanut variety registration, and the registration ID is \"GPD peanut (2018) 370105\". The results show that irradiation mutagenesis combined with tissue culture is an effective method for creating new germplasm and breeding new varieties of peanut.", "gemini": "Tạo dòng giống mới và lai tạo giống mới ở lạc bằng phương pháp đột biến bức xạ và nuôi cấy mô được tiến hành trong nghiên cứu này, nhằm mục đích xây dựng phương pháp lai tạo mới của lạc. Hạt chín từ Lạc hoa 11, giống lạc được trồng phổ biến nhất ở miền Bắc Trung Quốc, được xử lý bằng bức xạ neutron nhanh. Sau đó, các lá mầm phôi được tách ra khỏi hạt đã chiếu xạ và cấy trên môi trường, và cây con tái sinh thu được thông qua con đường sinh phôi soma. Cây con tái sinh được ghép, thích nghi rồi đem trồng ra ruộng và thu quả tự thụ từ 83 cây tái sinh. Các thế hệ sau được chọn lọc theo phương pháp phả hệ, và thu được 107 đột biến từ các thế hệ sau của 83 cây tái sinh. Các đột biến khác nhau cho thấy sự biến đổi rõ ràng về nhiều đặc điểm nông học, bao gồm chiều cao thân chính, số nhánh, hình dạng và kích thước quả, màu vỏ hạt, màu vỏ hạt bên trong, hàm lượng dầu và hàm lượng protein, v.v. Yuhua 7, một giống lạc mới có hàm lượng dầu thấp, chín sớm và chịu úng nước đã được thu được. Năng suất của Yuhua 7 cao hơn 14% so với giống bố mẹ đột biến Luhua 11, và hàm lượng dầu của hạt là 47,0%, thấp hơn so với giống bố mẹ Luhua 11 với hàm lượng dầu là 52,1%. Yuhua 7 đã vượt qua các thử nghiệm đa địa điểm khu vực về giống lạc ở tỉnh Liêu Ninh vào năm 2016 và năng suất trung bình của nó cao hơn 13,8% so với giống đối chứng Baisha 1017. Nó cũng đã vượt qua đăng ký giống lạc quốc gia, và ID đăng ký là \"Lạc GPD (2018) 370105\". Kết quả cho thấy phương pháp đột biến bức xạ kết hợp với nuôi cấy mô là một phương pháp hiệu quả để tạo dòng giống mới và lai tạo giống lạc mới."} +{"idx": 1890116, "en": "In previous studies, we found that truncated rotavirus VP4* (aa 26-476) could be expressed in soluble form in Escherichia coli and confer high protection against rotavirus in the mouse mode. In this study, we further improved the immunogenicity of VP4* by polymerization. The purified VP4* was polymerized through incubation at 37 ℃ for 24 h, and then the homogeneity of the particles was analyzed by HPLC, TEM and AUC, while the thermal stability and antigenicity was analyzed by DSC and ELISA, respectively. Finally, the immunogenicity and protective efficacy of the polymers analyzed by a mouse maternal antibody model. The results showed that VP4* aggregated into homogeneous polymers, with high thermostability and neutralizing antibody binding activity. In addition, VP4* polymers (endotoxin <20 EU/dose) stimulated higher neutralizing antibodies and confer higher protection against rotavirus-induced diarrhoea compared with the VP4* trimers when immunized with aluminium adjuvant. In summary, the study in VP4* polymers provides a new strategy for the development of recombinant rotavirus vaccines.", "gemini": "Trong các nghiên cứu trước đây, chúng tôi phát hiện ra rằng rotavirus VP4* bị cắt ngắn (aa 26-476) có thể được biểu hiện ở dạng hòa tan trong Escherichia coli và mang lại khả năng bảo vệ cao chống lại rotavirus ở chế độ chuột. Trong nghiên cứu này, chúng tôi đã cải thiện thêm tính miễn dịch của VP4* bằng cách trùng hợp. VP4* tinh khiết được trùng hợp thông qua quá trình ủ ở 37 ℃ trong 24 giờ, sau đó tính đồng nhất của các hạt được phân tích bằng HPLC, TEM và AUC, trong khi độ ổn định nhiệt và tính kháng nguyên được phân tích bằng DSC và ELISA, tương ứng. Cuối cùng, tính miễn dịch và hiệu quả bảo vệ của các polyme được phân tích bằng mô hình kháng thể mẹ chuột. Kết quả cho thấy VP4* kết tụ thành các polyme đồng nhất, có độ ổn nhiệt cao và hoạt tính gắn kháng thể trung hòa. Ngoài ra, các polyme VP4* (endotoxin <20 EU/liều) kích thích kháng thể trung hòa cao hơn và mang lại khả năng bảo vệ cao hơn chống lại tiêu chảy do rotavirus gây ra so với các trime VP4* khi được miễn dịch với tá dược nhôm. Tóm lại, nghiên cứu về polyme VP4* cung cấp một chiến lược mới cho sự phát triển của vắc-xin rotavirus tái tổ hợp."} +{"idx": 1890117, "en": "To investigate the effect of 27nt-miRNA on the differentiation of mesenchymal stem cells into vascular smooth muscle cells. The highly expression plasmids of 27nt-miRNA and anti-27nt-miRNA, and negative control plasmids were constructed, packaged with lentivirus and transfected into human umbilical cord mesenchymal stem cells (hUCMSCs). Collagen IV was added to induce hUCMSCs differentiation into blood vessel smooth muscle cells (VSMCs). The cell viability was measured by MTT assay. The expression of SMA, SM22α at mRNA and protein levels was determined by RT-PCR, immunocytochemical staining and Western blotting. Compared with the negative control group, the viability of the 27nt-miRNA overexpression group was decreased by 20.48% (P<0.05), and the expression of SMA mRNA and SM22α mRNA and protein was significantly increased (P<0.05); the viability of Anti-27nt-miRNA group was increased 18.07% (P<0.05), and the expression of SMA mRNA and SM22α mRNA and protein was decreased (P<0.05). In summary, 27nt-miRNA promotes mesenchymal stem cells differentiation into vascular smooth muscle cells and inhibits cells viability.", "gemini": "Để nghiên cứu tác dụng của 27nt-miRNA lên sự biệt hóa của tế bào gốc trung mô thành tế bào cơ trơn mạch máu. Các plasmid biểu hiện cao 27nt-miRNA và anti-27nt-miRNA, và các plasmid đối chứng âm tính đã được xây dựng, đóng gói với lentivirus và chuyển vào tế bào gốc trung mô dây rốn người (hUCMSC). Collagen IV đã được thêm vào để gây ra sự biệt hóa của hUCMSC thành tế bào cơ trơn mạch máu (VSMC). Độ sống của tế bào được đo bằng xét nghiệm MTT. Sự biểu hiện của SMA, SM22α ở mức mRNA và protein được xác định bằng RT-PCR, nhuộm miễn dịch tế bào và Western blotting. So với nhóm đối chứng âm tính, độ sống của nhóm quá biểu hiện 27nt-miRNA giảm 20,48% (P <0,05) và biểu hiện của mRNA SMA và mRNA SM22α và protein tăng đáng kể (P <0,05); độ sống của nhóm Anti-27nt-miRNA tăng 18,07% (P <0,05) và biểu hiện của mRNA SMA và mRNA SM22α và protein giảm (P <0,05). Tóm lại, 27nt-miRNA thúc đẩy sự biệt hóa của tế bào gốc trung mô thành tế bào cơ trơn mạch máu và ức chế độ sống của tế bào."} +{"idx": 1890118, "en": "The aim of the study was to obtain the secondary metabolites in the stem segment of noni and to establish genetic transformation system. The stem segments (no axillary buds) of noni were used as explants to induce the callus, and then to establish the cell suspension system. The factors affecting callus induction and cell suspension were studied. The results showed that the optimal culture medium for induction was MS with 1.0 mg/L 6-Benzylaminopurine (6-BA) and 0.1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and the optimum culture medium for suspension was MS with 1.0 mg/L 6-BA and 0.1 mg/L 2,4-D, 3% sucrose and the pH of 5.85, with the initial inoculation amount of 37.5 g/L, and the speed of 110 r/min and 25±2 °C applying darkness culture. The suspension cells grew well and showed the maximum growth rate. The growth curve of the suspension cells from the stem segment of noni was in \"S-typed\" trend, and it should be transformed to the fresh medium between 12 and 20 d. During the culture, the pH of the culture medium decreased and then slowly increased, and the optimum pH for the suspension cells culture of callus from noni's stem segments was 4.5-5.0. In this study, the stable cell suspension system of the stem segment of noni was successfully established.", "gemini": "Mục đích của nghiên cứu là để có được các chất chuyển hóa thứ cấp ở đoạn thân của chùm ngây và thiết lập hệ thống chuyển đổi gen. Các đoạn thân (không có chồi nách) của chùm ngây được sử dụng làm mẫu vật để gây ra mô sẹo, và sau đó để thiết lập hệ thống huyền phù tế bào. Các yếu tố ảnh hưởng đến việc gây ra mô sẹo và huyền phù tế bào đã được nghiên cứu. Kết quả cho thấy môi trường nuôi cấy tối ưu để gây ra là MS với 1,0 mg / L 6-Benzylaminopurine (6-BA) và 0,1 mg / L 2,4-dichlorophenoxyacetic acid (2,4-D), và môi trường nuôi cấy tối ưu cho huyền phù là MS với 1,0 mg / L 6-BA và 0,1 mg / L 2,4-D, 3% sucrose và độ pH 5,85, với lượng tiêm chủng ban đầu là 37,5 g / L và tốc độ 110 vòng / phút và 25 ± 2 ° C áp dụng nuôi cấy tối. Các tế bào huyền phù phát triển tốt và cho thấy tốc độ tăng trưởng tối đa. Đường cong tăng trưởng của các tế bào huyền phù từ đoạn thân của chùm ngây có xu hướng \"hình chữ S\" và nó phải được chuyển sang môi trường mới giữa 12 và 20 ngày. Trong quá trình nuôi cấy, độ pH của môi trường nuôi cấy giảm và sau đó tăng chậm, và độ pH tối ưu cho nuôi cấy tế bào huyền phù của mô sẹo từ các đoạn thân của chùm ngây là 4,5-5,0. Trong nghiên cứu này, hệ thống huyền phù tế bào ổn định của đoạn thân chùm ngây đã được thiết lập thành công."} +{"idx": 1890119, "en": "This study aimed to obtain a recombinant human-source collagen for industrialization. First, based on the Gly-X-Y sequence of human type I collagen, we optimized the hydrophilic Gly-X-Y collagen peptide, designed the human collagen amino acid sequence and the corresponding nucleotide sequence. Next, the expression vector pPIC9K-COL was constructed via endonuclease digestion technology. We obtained an engineering strain of human-source collagen by electrotransforming Pichia pastoris, and then it was fermented, purified and identified. As a result, the expression level reached 4.5 g/L and the purity was over 95%. After amino acid N-terminal sequencing, molecular weight analysis, amino acid analysis and collagenase degradation test, we confirmed that the obtained collagen was consistent with designed primary structure of human-source collagen. After freeze-drying, we analyzed the collagen by scanning electron microscope and cell cytotoxicity, confirming that the collagen has porous fiber reticular structure and superior cytocompatibility. This indicates that human-source collagen has potential to be applied as biomedical material. In conclusion, we successfully obtained the expected human-source collagen and laid a foundation to its further application.", "gemini": "Nghiên cứu này nhằm mục đích thu được collagen nguồn gốc từ con người tái tổ hợp để công nghiệp hóa. Đầu tiên, dựa trên trình tự Gly-X-Y của collagen loại I của người, chúng tôi đã tối ưu hóa peptide collagen Gly-X-Y ưa nước, thiết kế trình tự axit amin collagen của người và trình tự nucleotide tương ứng. Tiếp theo, vectơ biểu hiện pPIC9K-COL được xây dựng thông qua công nghệ tiêu hóa bằng enzyme cắt giới hạn. Chúng tôi đã thu được một chủng kỹ thuật collagen nguồn gốc từ người bằng cách chuyển đổi điện Pichia pastoris, sau đó lên men, tinh chế và xác định. Kết quả là mức biểu hiện đạt 4,5 g / L và độ tinh khiết trên 95%. Sau khi giải trình tự đầu N axit amin, phân tích khối lượng phân tử, phân tích axit amin và thử nghiệm phân hủy collagenase, chúng tôi xác nhận rằng collagen thu được phù hợp với cấu trúc chính được thiết kế của collagen nguồn gốc từ người. Sau khi sấy đông khô, chúng tôi phân tích collagen bằng kính hiển vi điện tử quét và độc tính tế bào, xác nhận rằng collagen có cấu trúc lưới sợi xốp và khả năng tương thích tế bào cao. Điều này chỉ ra rằng collagen nguồn gốc từ người có tiềm năng được ứng dụng làm vật liệu y sinh. Tóm lại, chúng tôi đã thành công trong việc thu được collagen nguồn gốc từ người như mong đợi và đặt nền tảng cho ứng dụng tiếp theo của nó."} +{"idx": 1890120, "en": "Nowadays, available phosphorus (P) deficiency in soil and weed resistance to herbicides have emerged as two severe limiting factors for sustainable agriculture. Therefore, it is of urgent needs to improve plant absorption/utilization ability of the soil P, seek phosphate (Pi)-alternative P fertilizers, and develop new forms of weed control systems. Phosphite (Phi), as a P resource of relatively high amount only less than Pi in Earth, can be converted to utilizable Pi uniquely in some bacterial species by oxidization via its specific dehydrogenase (PTDH), but inhibits plant growth and development. This implies that Phi might rather become a suitable P fertilizer for plants if introducing a PTDH detoxifier from bacteria. Herein, we created the transgenic tobaccos harboring a Pseudomonas PTDH gene (PsPtx) amplified from the soil metagenome previously. RT-PCR showed that the exotic PsPtx gene could express similarly in root, stem and leaf tissues of all transgenic lines. PsPtx transgenic tobaccos could utilize Phi by oxidization as the sole Pi supply, and also outperformed wild-type tobacco with a remarkably dominant growth under Phi stress conditions. Moreover, the PsPtx gene was preliminarily evaluated with a notable quality as a potential candidate of the selection marker in plant genetic transformation. Conclusively, PsPtx and its encoded phosphite dehydrogenase might be applicable for developing a dual system of plant phosphorus utilization and weed control using Phi as P fertilizer and herbicide, and provide an effectual solution to some obstacles in the current crop transgenic studies.", "gemini": "Ngày nay, tình trạng thiếu phốt pho (P) có sẵn trong đất và tình trạng kháng thuốc diệt cỏ của cỏ dại đã trở thành hai yếu tố hạn chế nghiêm trọng đối với nền nông nghiệp bền vững. Do đó, việc cải thiện khả năng hấp thụ/sử dụng P trong đất của cây trồng, tìm kiếm các loại phân bón P thay thế photphat (Pi) và phát triển các hình thức hệ thống kiểm soát cỏ dại mới là những nhu cầu cấp thiết. Photphite (Phi), là một nguồn P có hàm lượng tương đối cao chỉ kém Pi trong Trái đất, có thể được chuyển đổi thành Pi có thể sử dụng được một cách độc đáo ở một số loài vi khuẩn bằng cách oxy hóa thông qua dehydrogenase đặc hiệu (PTDH) của nó, nhưng lại ức chế sự tăng trưởng và phát triển của cây trồng. Điều này ngụ ý rằng Phi có thể trở thành một loại phân bón P phù hợp cho cây trồng nếu đưa vào một chất giải độc PTDH từ vi khuẩn. Trong nghiên cứu này, chúng tôi đã tạo ra thuốc lá chuyển gen chứa gen PTDH của Pseudomonas (PsPtx) được khuếch đại từ metagenome đất trước đó. RT-PCR cho thấy gen PsPtx ngoại lai có thể biểu hiện tương tự ở mô rễ, thân và lá của tất cả các dòng chuyển gen. Thuốc lá chuyển gen PsPtx có thể sử dụng Phi bằng cách oxy hóa như nguồn cung cấp Pi duy nhất, và cũng vượt trội hơn thuốc lá kiểu hoang dã với sự tăng trưởng vượt trội đáng kể trong điều kiện căng thẳng Phi. Hơn nữa, gen PsPtx đã được đánh giá sơ bộ với chất lượng đáng chú ý như một ứng cử viên tiềm năng của gen đánh dấu chọn lọc trong chuyển đổi gen thực vật. Tóm lại, PsPtx và photphite dehydrogenase được mã hóa của nó có thể được áp dụng để phát triển hệ thống kép sử dụng phốt pho của cây trồng và kiểm soát cỏ dại bằng cách sử dụng Phi làm phân bón P và thuốc diệt cỏ, đồng thời cung cấp giải pháp hiệu quả cho một số trở ngại trong các nghiên cứu chuyển gen cây trồng hiện nay."} +{"idx": 1890121, "en": "Aim: The purpose of this study is to evaluate the prevalence of the immunohistochemical subtypes of breast cancer among Lao women by using immunohistochemistry (according to the St. Gallen 2017 guidelines) and to study their correlation to clinicopathological features in order to help guide better treatment plans for patients. Materials and methods: Formalin-fixed and paraffin embedded tissue blocks of 76 cases of primary invasive breast cancer were retrieved from the University of Health Sciences, Vientiane, Lao PDR, from 2013 to 2016. Patients’ information and previous histological reports were reviewed. Immunohistochemistry was done using antibodies against estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2/neu) and Ki-67 (MIB-1). Results: The mean age of the patients was 49 years, and the major histologic type was invasive ductal carcinoma, NOS (90.7%). The proportion of each subtype was hormone receptor-positive and HER2-negative, 44.7%; hormone receptor-positive and HER2-positive, 3.9%; hormone receptor-negative and HER2-positive, 13.2%; and triple-negative, 38.2%. ER was positive in 40.8% of the cases, while PR was positive in 47.4%. More than half of the cases were poorly differentiated cancer (65.8%), followed by moderately differentiated (34.2%). Tumors presented with pT2 (60.5%), followed by pT3 (25.0%) and pT4 (7.9%). Conclusion: Breast cancer among Lao women is characterized by a large percentage of the triple-negative subtype that is less susceptible to hormonal treatments. The empirical treatment with tamoxifen should be reconsidered since it would be less effective to these patients. More importantly, basic pathology services should be the first requirement in Lao PDR in order to provide adequate care.", "gemini": "Mục tiêu: Mục đích của nghiên cứu này là đánh giá tỷ lệ mắc các phân nhóm miễn dịch mô hóa học của ung thư vú ở phụ nữ Lào bằng cách sử dụng miễn dịch mô hóa học (theo hướng dẫn của St. Gallen 2017) và nghiên cứu mối tương quan của chúng với các đặc điểm lâm sàng và bệnh lý để giúp hướng dẫn các kế hoạch điều trị tốt hơn cho bệnh nhân. Vật liệu và phương pháp: Các khối mô được cố định bằng formalin và nhúng paraffin của 76 trường hợp ung thư vú xâm lấn nguyên phát đã được lấy từ Đại học Khoa học Sức khỏe, Vientiane, Cộng hòa Dân chủ Nhân dân Lào, từ năm 2013 đến năm 2016. Thông tin bệnh nhân và các báo cáo mô học trước đó đã được xem xét. Miễn dịch mô hóa học đã được thực hiện bằng cách sử dụng kháng thể chống lại thụ thể estrogen (ER), thụ thể progesterone (PR), thụ thể yếu tố tăng trưởng biểu bì ở người 2 (HER2/neu) và Ki-67 (MIB-1). Kết quả: Tuổi trung bình của bệnh nhân là 49 tuổi và loại mô học chính là ung thư biểu mô ống xâm lấn, NOS (90,7%). Tỷ lệ của từng phân nhóm là thụ thể hormone dương tính và HER2 âm tính, 44,7%; thụ thể hormone dương tính và HER2 dương tính, 3,9%; thụ thể hormone âm tính và HER2 dương tính, 13,2%; và ba âm tính, 38,2%. ER dương tính ở 40,8% các trường hợp, trong khi PR dương tính ở 47,4%. Hơn một nửa số trường hợp là ung thư biệt hóa kém (65,8%), tiếp theo là biệt hóa trung bình (34,2%). Các khối u có pT2 (60,5%), tiếp theo là pT3 (25,0%) và pT4 (7,9%). Kết luận: Ung thư vú ở phụ nữ Lào được đặc trưng bởi tỷ lệ phần trăm lớn của phân nhóm ba âm tính, ít nhạy cảm hơn với các phương pháp điều trị bằng nội tiết tố. Cần xem xét lại việc điều trị theo kinh nghiệm bằng tamoxifen vì nó sẽ ít hiệu quả hơn đối với những bệnh nhân này. Quan trọng hơn, các dịch vụ bệnh lý cơ bản nên là yêu cầu đầu tiên ở Cộng hòa Dân chủ Nhân dân Lào để cung cấp dịch vụ chăm sóc đầy đủ."} +{"idx": 1890122, "en": "Introduction: Previous studies comparing tomotherapy (TOMO) and three dimensional (3D) conformal radiotherapy (3DCRT) in gastric radiotherapy are limited and tend to be based on dosimetry. The aim of the present study was to evaluate the clinical outcomes of these two treatment modalities. Methods: A total of 51 patients diagnosed with gastric cancer who were treated with postoperative adjuvant chemoradiotherapy and had subtotal/total gastrectomy and D2 lymphatic dissection were recruited to the present study: 30 patients were treated with TOMO and 21 patients were treated with 3DCRT. Results: The 3DCRT and TOMO treatment regimens were compared. There was no difference in planning target volume (PTV) 95%, but TOMO was statistically significant in regard to PTV 105% (P<0.05). TOMO was also significantly different when compared with 3DCRT when evaluating liver mean dose, liver V40, right/left kidneys mean dose, right/left kidneys V20 and spinal cord mean dose values (P<0.05). Grade 2 acute side effects were more frequent (85.7%) following 3DCRT. In addition, the median overall survival time for TOMO treated patients was 62 months while in 3DCRT treated patients it was 22.05 months. The difference in disease free survival was also significantly increased in patients treated with TOMO (66.7% vs. 19.0%; P<0.05). Conclusion: TOMO treatment resulted in lower acute side effects with better patient survival following gastric cancer radiotherapy.", "gemini": "Mở đầu: Các nghiên cứu trước đây so sánh xạ trị cắt lớp vi tính (TOMO) và xạ trị chuẩn ba chiều (3DCRT) trong xạ trị dạ dày còn hạn chế và có xu hướng dựa trên liều lượng. Mục đích của nghiên cứu hiện tại là đánh giá kết quả lâm sàng của hai phương thức điều trị này. Phương pháp: Tổng cộng 51 bệnh nhân được chẩn đoán mắc ung thư dạ dày được điều trị bằng hóa xạ trị bổ trợ sau phẫu thuật và cắt dạ dày bán phần/toàn phần và cắt hạch bạch huyết D2 đã được tuyển dụng vào nghiên cứu hiện tại: 30 bệnh nhân được điều trị bằng TOMO và 21 bệnh nhân được điều trị bằng 3DCRT. Kết quả: Các chế độ điều trị 3DCRT và TOMO đã được so sánh. Không có sự khác biệt về thể tích mục tiêu lập kế hoạch (PTV) 95%, nhưng TOMO có ý nghĩa thống kê về PTV 105% (P < 0,05). TOMO cũng có sự khác biệt đáng kể khi so sánh với 3DCRT khi đánh giá liều trung bình của gan, V40 của gan, liều trung bình của thận phải/trái, V20 của thận phải/trái và giá trị liều trung bình của tủy sống (P < 0,05). Các tác dụng phụ cấp độ 2 thường gặp hơn (85,7%) sau 3DCRT. Ngoài ra, thời gian sống sót toàn thể trung bình đối với bệnh nhân được điều trị bằng TOMO là 62 tháng trong khi ở bệnh nhân được điều trị bằng 3DCRT là 22,05 tháng. Sự khác biệt về thời gian sống không có bệnh cũng tăng đáng kể ở những bệnh nhân được điều trị bằng TOMO (66,7% so với 19,0%; P < 0,05). Kết luận: Điều trị TOMO dẫn đến tác dụng phụ cấp tính thấp hơn với khả năng sống sót của bệnh nhân tốt hơn sau xạ trị ung thư dạ dày."} +{"idx": 1890123, "en": "Objective: This study was conducted to investigate the antiproliferative activity of extracts of Clinacanthus nutans leaves against human cervical cancer (HeLa) cells. Methods: C. nutans leaves were subjected to extraction using 80% methanol or water. The methanol extract was further extracted to obtain hexane, dichloromethane (DCM), and aqueous fractions. The antiproliferative activity of the extracts against HeLa cells was determined. The most cytotoxic extract was furthered analyzed by apoptosis and cell cycle assays, and the phytochemical constituents were screened by gas chromatography-mass spectrometry (GC-MS). Results: All of the extracts were antiproliferative against HeLa cells, and the DCM fraction had the lowest IC50 value of 70 μg/mL at 48 h. Microscopic studies showed that HeLa cells exposed to the DCM fraction exhibited marked morphological features of apoptosis. The flow cytometry study also confirmed that the DCM fraction induced apoptosis in HeLa cells, with cell cycle arrest at the S phase. GC-MS analysis revealed the presence of at least 28 compounds in the DCM fraction, most of which were fatty acids. Conclusion: The DCM fraction obtained using the extraction method described herein had a lower IC50 value than those reported in previous studies that characterized the anticancer activity of C. nutans against HeLa cells.", "gemini": "Mục tiêu: Nghiên cứu này được tiến hành để tìm hiểu hoạt động chống tăng sinh của chiết xuất từ lá cây Clinacanthus nutans đối với tế bào ung thư cổ tử cung ở người (HeLa). Phương pháp: Lá C. nutans được chiết xuất bằng 80% methanol hoặc nước. Chiết xuất methanol được chiết xuất thêm để thu được hexane, dichloromethane (DCM) và các phân đoạn nước. Hoạt động chống tăng sinh của các chiết xuất đối với tế bào HeLa đã được xác định. Chiết xuất độc tế bào nhất được phân tích thêm bằng xét nghiệm apoptosis và chu kỳ tế bào, và các thành phần hóa học thực vật được sàng lọc bằng sắc ký khí khối phổ (GC-MS). Kết quả: Tất cả các chiết xuất đều có tác dụng chống tăng sinh đối với tế bào HeLa và phân đoạn DCM có giá trị IC50 thấp nhất là 70 μg / mL tại 48 giờ. Các nghiên cứu trên kính hiển vi cho thấy các tế bào HeLa tiếp xúc với phân đoạn DCM biểu hiện các đặc điểm hình thái rõ nét của apoptosis. Nghiên cứu đo lưu lượng tế bào cũng xác nhận rằng phân đoạn DCM gây apoptosis ở tế bào HeLa, với sự bắt giữ chu kỳ tế bào ở pha S. Phân tích GC-MS cho thấy sự hiện diện của ít nhất 28 hợp chất trong phân đoạn DCM, hầu hết trong số đó là axit béo. Kết luận: Phân đoạn DCM thu được bằng phương pháp chiết xuất được mô tả ở đây có giá trị IC50 thấp hơn so với các nghiên cứu trước đây đã mô tả hoạt động chống ung thư của C. nutans đối với tế bào HeLa."} +{"idx": 1890124, "en": "Background: Breast cancer is the most common cancer diagnosed among women, Tumor suppressor genes such as BRCA1 involved in cell cycle control and repairing of DNA damage. BRCA1 is a risk factor gene that alteration in its protein cause in susceptibility to breast or ovarian cancer. Short tandem repeat (STR) polymorphism is linked to some disease. Objective: The aim of this study was screening a new mutation in patients with familial breast cancer. Materials and Methods: In this study, 200 women with breast cancer were participated. Among the patients, 40 women suffer from familial breast cancer. After DNA extraction from peripheral blood samples, Exons 16 to 23 of BRCA1 gene directly analyzed in SSCP gel electrophoresis followed by direct sequencing. Results: After direct sequencing, a new mutation was detected in intron 17 of BRCA1 gene. Three patients of one family have a germ line intronic mutation in the BRCA1 gene (IVS17-27delA). Also, this mutation in this family is linked to a haplotype of intragenic short tandem repeat (STR) in the BRCA1 gene. Conclusion: By Screening of gene mutations can be found association of mutation and incidence of disease. Also, studying the mutation in families and finding specific hereditary patterns in that family can be effective in prognosis of disease in other family members.", "gemini": "Bối cảnh: Ung thư vú là loại ung thư phổ biến nhất được chẩn đoán ở phụ nữ, gen ức chế khối u như BRCA1 liên quan đến kiểm soát chu kỳ tế bào và sửa chữa tổn thương DNA. BRCA1 là một gen yếu tố rủi ro mà sự thay đổi trong protein của nó gây ra tình trạng dễ mắc ung thư vú hoặc ung thư buồng trứng. Đa hình lặp lại ngắn (STR) có liên quan đến một số bệnh. Mục tiêu: Mục đích của nghiên cứu này là sàng lọc một đột biến mới ở những bệnh nhân ung thư vú gia đình. Vật liệu và phương pháp: Trong nghiên cứu này, 200 phụ nữ bị ung thư vú đã tham gia. Trong số những bệnh nhân này, 40 phụ nữ mắc ung thư vú gia đình. Sau khi trích xuất DNA từ các mẫu máu ngoại vi, các exon 16 đến 23 của gen BRCA1 được phân tích trực tiếp trong điện di gel SSCP sau đó giải trình tự trực tiếp. Kết quả: Sau khi giải trình tự trực tiếp, một đột biến mới đã được phát hiện ở intron 17 của gen BRCA1. Ba bệnh nhân trong một gia đình có đột biến intron dòng mầm trong gen BRCA1 (IVS17-27delA). Ngoài ra, đột biến này trong gia đình này có liên quan đến kiểu gen lặp lại ngắn trong gen (STR) trong gen BRCA1. Kết luận: Bằng cách sàng lọc các đột biến gen có thể tìm thấy mối liên quan giữa đột biến và tỷ lệ mắc bệnh. Ngoài ra, việc nghiên cứu đột biến trong các gia đình và tìm ra các kiểu di truyền cụ thể trong gia đình đó có thể có hiệu quả trong việc tiên lượng bệnh ở các thành viên khác trong gia đình."} +{"idx": 1890125, "en": "Background: Prostate cancer (PCa) is a common health problem in elderly. RAGE (Receptor for advanced glycation end products) is overexpressed in multiple human cancers. SOX2 (Sex-determining region Y box 2) also functions as an oncoprotein and promotes cancer progression but the mechanisms involved remain largely unknown. Aim: The current study investigated the expression patterns of RAGE and SOX2 in benign and malignant prostate samples in correlation with the histopathological findings in order to evaluate their role as prognostic markers or therapeutic targets. Methods: Immunohistochemical staining for RAGE and SOX2 antibodies was applied on 87 prostatic biopsies [16 of prostatitis, 20 of benign prostatic hyperplasia (BPH) and 51 of PCa]. Results: Expression of RAGE and SOX2 (percentage of positive cells) was significantly higher in PCa lesions compared with prostatitis (p<0.01) and BPH (p<0.0001) and was also significantly higher in prostatitis compared with BPH lesions (p<0.01). Also, percentage of positive RAGE and SOX2 cells showed a significant stepwise increase from Gleason Grade 3 to Grade 5 and were significantly higher in high Gleason Scores (≥8) compared to lower Scores (≤7) with statistical significance (p=0.001). Conclusion: RAGE and SOX2 were up-regulated in prostate cancer lesions, mainly in advanced grades, suggesting an active role of both antigens in the development and progression of prostate cancer and expecting the possibility of their use as therapeutic targets.", "gemini": "Bối cảnh: Ung thư tuyến tiền liệt (PCa) là một vấn đề sức khỏe phổ biến ở người cao tuổi. RAGE (Receptor for advanced glycation end products) được biểu hiện quá mức trong nhiều loại ung thư của con người. SOX2 (Sex-determining region Y box 2) cũng hoạt động như một oncoprotein và thúc đẩy sự tiến triển của ung thư nhưng các cơ chế liên quan vẫn chưa được biết rõ. Mục đích: Nghiên cứu hiện tại đã điều tra các kiểu biểu hiện của RAGE và SOX2 trong các mẫu tuyến tiền liệt lành tính và ác tính có tương quan với các phát hiện bệnh học để đánh giá vai trò của chúng như các dấu ấn tiên lượng hoặc mục tiêu điều trị. Phương pháp: Nhuộm miễn dịch mô cho kháng thể RAGE và SOX2 đã được áp dụng trên 87 mẫu sinh thiết tuyến tiền liệt [16 viêm tuyến tiền liệt, 20 u xơ tuyến tiền liệt lành tính (BPH) và 51 PCa]. Kết quả: Biểu hiện của RAGE và SOX2 (tỷ lệ phần trăm tế bào dương tính) cao hơn đáng kể ở các tổn thương PCa so với viêm tuyến tiền liệt (p <0,01) và BPH (p <0,0001) và cũng cao hơn đáng kể ở viêm tuyến tiền liệt so với các tổn thương BPH (p <0,01). Ngoài ra, tỷ lệ phần trăm tế bào RAGE và SOX2 dương tính cho thấy sự gia tăng từng bước đáng kể từ Gleason Grade 3 đến Grade 5 và cao hơn đáng kể ở Gleason Scores cao (≥8) so với Scores thấp hơn (≤7) với ý nghĩa thống kê (p = 0,001). Kết luận: RAGE và SOX2 được điều chỉnh tăng lên ở các tổn thương ung thư tuyến tiền liệt, chủ yếu ở các loại tiến triển, cho thấy vai trò tích cực của cả hai kháng nguyên trong sự phát triển và tiến triển của ung thư tuyến tiền liệt và mong đợi khả năng sử dụng chúng làm mục tiêu điều trị."} +{"idx": 1890126, "en": "Objectives: To assess the efficacy of cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) in recurrent platinum-sensitive ovarian cancer patients in comparison with standard intravenous chemotherapy in terms of progression free survival and overall survival. Methods: Retrospective case control study matching 15 cases with 20 controls with at least 24 months of follow up. Results: The two groups were comparable and well matched in all aspects. Median follow up was 36 months in cases and 38 months in controls. The PFS2 revealed a median of 6 months (range 2-14) in cases and 5 months (range 2-18) in controls. The median OS was 36 and 38 months in cases and controls respectively. No statistically significant difference between the cases and controls were observed in progression free survival (PFS2) and overall survival OS (P-value, 0.350 and 0.711 respectively). However, the PFS2 was in favor of cases and OS was in favor of controls without reaching significance. The percentage of patients who survived 5 years or more was 20% in cases and 35% in controls. The only issue in favor of HIPEC is the significant reduction in chemotherapeutic toxicity when given by the intraperitoneal way (P- value 0.003). Conclusion: According to our study, CRS and HIPEC do not seem to have impact on OS and PFS in the setting of recurrent platinum sensitive ovarian cancer. However, we recommend on going researches with much more refined selection criteria and with larger sample size.", "gemini": "Mục tiêu: Đánh giá hiệu quả của phẫu thuật cắt giảm khối u (CRS) kết hợp với hóa trị nội phúc mạc tăng nhiệt (HIPEC) ở bệnh nhân ung thư buồng trứng nhạy cảm với bạch kim tái phát so với hóa trị tĩnh mạch tiêu chuẩn về thời gian sống không tiến triển và thời gian sống toàn thể. Phương pháp: Nghiên cứu đối chứng theo trường hợp hồi cứu kết hợp 15 trường hợp với 20 đối chứng có thời gian theo dõi ít nhất 24 tháng. Kết quả: Hai nhóm có thể so sánh và phù hợp ở mọi khía cạnh. Thời gian theo dõi trung vị là 36 tháng ở các trường hợp và 38 tháng ở các đối chứng. PFS2 cho thấy thời gian sống trung vị là 6 tháng (phạm vi 2-14) ở các trường hợp và 5 tháng (phạm vi 2-18) ở các đối chứng. OS trung bình là 36 và 38 tháng ở các trường hợp và đối chứng tương ứng. Không có sự khác biệt có ý nghĩa thống kê giữa các trường hợp và đối chứng về thời gian sống không tiến triển (PFS2) và thời gian sống toàn thể OS (Giá trị P lần lượt là 0,350 và 0,711). Tuy nhiên, PFS2 có lợi cho các trường hợp và OS có lợi cho các đối chứng nhưng không đạt mức có ý nghĩa. Tỷ lệ bệnh nhân sống sót 5 năm trở lên là 20% ở các trường hợp và 35% ở các đối chứng. Vấn đề duy nhất có lợi cho HIPEC là làm giảm đáng kể độc tính của hóa trị khi dùng theo đường nội phúc mạc (Giá trị P là 0,003). Kết luận: Theo nghiên cứu của chúng tôi, CRS và HIPEC dường như không ảnh hưởng đến OS và PFS trong bối cảnh ung thư buồng trứng nhạy cảm với bạch kim tái phát. Tuy nhiên, chúng tôi khuyến cáo nên tiếp tục các nghiên cứu với các tiêu chí lựa chọn được tinh chỉnh hơn nhiều và với quy mô mẫu lớn hơn."} +{"idx": 1890127, "en": "Background: The purpose of the research was to improve the polyp detection accuracy in CT Colonography (CTC) through effective colon segmentation, removal of tagged fecal matter through Electronic Cleansing (EC), and measuring the smaller polyps. Methods: An improved method of boundary-based semi-automatic colon segmentation with the knowledge of colon distension, an adaptive multistep method for the virtual cleansing of segmented colon based on the knowledge of Hounsfield Units, and an automated method of smaller polyp measurement using skeletonization technique have been implemented. Results: The techniques were evaluated on 40 CTC dataset. The segmentation method was able to delineate the colon wall accurately. The submerged colonic structures were preserved without soft tissue erosion, pseudo enhanced voxels were corrected, and the air-contrast layer was removed without losing the adjacent tissues. The smaller polyp of size less than <10mm was detected correctly. The results were statistically validated qualitatively and quantitatively. Segmented colons were validated through volumetric overlap computation, and accuracy of 95.826±0.6854% was achieved. In polyp measurement, the paired t-test method was applied to compare the difference with ground truth and at α=5%, t=0.9937 and p=0.098 was achieved. The statistical values of TPR=90%, TNR=82.3% and accuracy=88.31% were achieved. Conclusion: An automated system of polyp measurement has been developed starting from colon segmentation to improve the existing CTC solutions. The analysis of domain-based approach of polyp has given good results. A prototype software, which can be used as a low-cost polyp diagnosis tool, has been developed.", "gemini": "Bối cảnh: Mục đích của nghiên cứu là cải thiện độ chính xác phát hiện polyp trong chụp cắt lớp đại tràng (CTC) thông qua việc phân đoạn đại tràng hiệu quả, loại bỏ phân được gắn thẻ thông qua làm sạch điện tử (EC) và đo các polyp nhỏ hơn. Phương pháp: Một phương pháp cải tiến phân đoạn đại tràng bán tự động dựa trên ranh giới với kiến thức về sự giãn nở của đại tràng, một phương pháp đa bước thích ứng để làm sạch ảo đại tràng được phân đoạn dựa trên kiến thức về Đơn vị Hounsfield và một phương pháp tự động đo polyp nhỏ hơn sử dụng kỹ thuật tạo bộ xương đã được triển khai. Kết quả: Các kỹ thuật đã được đánh giá trên 40 tập dữ liệu CTC. Phương pháp phân đoạn có thể mô tả chính xác thành đại tràng. Các cấu trúc đại tràng chìm được bảo tồn mà không bị xói mòn mô mềm, các voxel được tăng cường giả được hiệu chỉnh và lớp tương phản không khí được loại bỏ mà không làm mất các mô liền kề. Polyp nhỏ hơn có kích thước nhỏ hơn <10mm đã được phát hiện chính xác. Kết quả được xác nhận về mặt thống kê cả về mặt định tính và định lượng. Các đại tràng được phân đoạn đã được xác nhận thông qua tính toán chồng chéo theo thể tích và đạt được độ chính xác là 95,826 ± 0,6854%. Trong phép đo polyp, phương pháp kiểm định t ghép đôi đã được áp dụng để so sánh sự khác biệt với giá trị thực và ở α = 5%, đạt được t = 0,9937 và p = 0,098. Các giá trị thống kê của TPR = 90%, TNR = 82,3% và độ chính xác = 88,31% đã đạt được. Kết luận: Một hệ thống tự động đo polyp đã được phát triển bắt đầu từ phân đoạn đại tràng để cải thiện các giải pháp CTC hiện có. Phân tích phương pháp tiếp cận polyp dựa trên miền đã đưa ra kết quả tốt. Một phần mềm nguyên mẫu, có thể được sử dụng như một công cụ chẩn đoán polyp chi phí thấp, đã được phát triển."} +{"idx": 1890128, "en": "Melastoma malabathricum L. is a type of plant naturally grows in Kalimantan that has medicinal properties. Ethanolic extract of M. malabathricum L. flower has quercetin and kaempferol contents that have antioxidant and anticancer activities. But the antioxidant and cytotoxicity activities of M. malabathricum L. fruit ethanolic extract were not known. This study measured the quercetin and kaempferol level in M. malabathricum L. fruit ethanolic extract using HPLC MS/MS, antioxidant activity using DPPH method, and cytotoxicity activity using Brine Shrimp Lethality Test (BSLT) method. Results showed the level of quercetin and kaempferol from M. malabathricum L fruit ethanolic extract are 67.78 μg/g and 43.52 μg/g, respectively. Beside, the antioxidant activity with by IC50 was 16.82±0.24 ppm and 7.38±0.41 ppm. The cytotoxicity activities of M. malabathricum L. fruit ethanolic extract and quercetin are shown by the LC50 of 313.44 ppm (95%CI 283.97-344.43) and 37.24 ppm, respectively.", "gemini": "Melastoma malabathricum L. là một loại cây mọc tự nhiên ở Kalimantan có đặc tính dược liệu. Chiết xuất ethanol từ hoa M. malabathricum L. có chứa quercetin và kaempferol có hoạt tính chống oxy hóa và chống ung thư. Nhưng hoạt tính chống oxy hóa và độc tế bào của chiết xuất ethanol từ quả M. malabathricum L. vẫn chưa được biết đến. Nghiên cứu này đo nồng độ quercetin và kaempferol trong chiết xuất ethanol từ quả M. malabathricum L. bằng HPLC MS/MS, hoạt tính chống oxy hóa bằng phương pháp DPPH và hoạt tính độc tế bào bằng phương pháp thử độc tính trên tôm ngâm nước muối (BSLT). Kết quả cho thấy hàm lượng quercetin và kaempferol từ chiết xuất ethanol từ quả M. malabathricum L lần lượt là 67,78 μg/g và 43,52 μg/g. Bên cạnh đó, hoạt tính chống oxy hóa với IC50 lần lượt là 16,82 ± 0,24 ppm và 7,38 ± 0,41 ppm. Hoạt tính độc tế bào của chiết xuất ethanol từ quả M. malabathricum L. và quercetin được thể hiện bằng LC50 lần lượt là 313,44 ppm (95%CI 283,97-344,43) và 37,24 ppm."} +{"idx": 1890129, "en": "Background: Colorectal cancer is an important public health problem worldwide. Although progress in screening and treatment has considerably improved the prognosis in the developed world, in developing countries colorectal cancer mortality rate remains relatively high. Colorectal cancer screening literacy is an important initial step in overcoming this problem. Development of a validated assessment instrument is therefore important for implementation of appropriate health education programs to facilitate early detection. Objectives: This study focused on generation and validation of a colorectal cancer screening literacy scale for Thai people in northeastern Thailand. Methods: This methodological study was carried out in two phases: (1) literature reviews and semi-structured interviews were used to select items, then the content and face validity were checked; and (2) a confirmatory factor analysis (CFA) was conducted to test construct validity and reliability. A self-administered questionnaire was used to collect data from Thai people aged 50- 65 in June 2017. Results: For the total of 400 participants who responded (response rate 100 %), the age ranged from 50 to 65 years old (mean = 57.3, SD = 4.616). The colorectal cancer screening literacy scale was designed to include 6 domains and it was shown to have good internal consistency, and CFA demonstrated the model to fit data adequately (Chi-squared/degree of freedom = 1.079, p = 0.061, CFI = 1.00, GFI = 0.93, AGFI = 0.91, RMSEA = 0.014 and SRMR = 0.036). The final version of its, consisting of 57 items across the 6 domains covering key aspects of colorectal cancer screening literacy, demonstrated good psychometric properties for this population. Conclusions: Use of the colorectal cancer screening literacy scale in Thai people could lead to improved educational programs for optimizing colorectal cancer screening.", "gemini": "Bối cảnh: Ung thư đại trực tràng là một vấn đề sức khỏe cộng đồng quan trọng trên toàn thế giới. Mặc dù tiến bộ trong sàng lọc và điều trị đã cải thiện đáng kể tiên lượng ở các nước phát triển, nhưng ở các nước đang phát triển, tỷ lệ tử vong do ung thư đại trực tràng vẫn tương đối cao. Kiến thức về sàng lọc ung thư đại trực tràng là một bước ban đầu quan trọng để khắc phục vấn đề này. Do đó, việc phát triển một công cụ đánh giá được xác nhận là rất quan trọng để thực hiện các chương trình giáo dục sức khỏe phù hợp nhằm tạo điều kiện phát hiện sớm. Mục tiêu: Nghiên cứu này tập trung vào việc tạo ra và xác nhận thang đánh giá kiến thức sàng lọc ung thư đại trực tràng cho người Thái ở đông bắc Thái Lan. Phương pháp: Nghiên cứu phương pháp luận này được thực hiện theo hai giai đoạn: (1) xem xét tài liệu và phỏng vấn bán cấu trúc được sử dụng để lựa chọn các mục, sau đó nội dung và tính hợp lệ được kiểm tra; và (2) một phân tích nhân tố xác nhận (CFA) đã được tiến hành để kiểm tra tính hợp lệ và độ tin cậy của cấu trúc. Một bảng câu hỏi tự trả lời đã được sử dụng để thu thập dữ liệu từ người Thái trong độ tuổi 50-65 vào tháng 6 năm 2017. Kết quả: Đối với tổng số 400 người tham gia trả lời (tỷ lệ phản hồi 100%), độ tuổi từ 50 đến 65 tuổi (trung bình = 57,3, SD = 4,616). Thang đánh giá kiến thức sàng lọc ung thư đại trực tràng được thiết kế để bao gồm 6 phạm vi và cho thấy có tính nhất quán nội bộ tốt và CFA chứng minh mô hình phù hợp với dữ liệu đầy đủ (Chi bình phương/mức độ tự do = 1,079, p = 0,061, CFI = 1,00, GFI = 0,93, AGFI = 0,91, RMSEA = 0,014 và SRMR = 0,036). Phiên bản cuối cùng của nó, bao gồm 57 mục trên 6 phạm vi bao gồm các khía cạnh chính của kiến thức sàng lọc ung thư đại trực tràng, đã chứng minh các đặc tính trắc nghiệm tốt cho quần thể này. Kết luận: Việc sử dụng thang đánh giá kiến thức sàng lọc ung thư đại trực tràng ở người Thái có thể dẫn đến các chương trình giáo dục được cải thiện để tối ưu hóa sàng lọc ung thư đại trực tràng."} +{"idx": 1890130, "en": "OBJECTIVE Group-based intervention formats are common in rehabilitation, but no tool for objectively measuring clinical competencies in group facilitation currently exists. We aimed to develop a psychometrically sound group facilitation competency checklist for use in clinical, training, and research settings. METHOD The Delphi method of expert consensus was used to establish checklist items that clearly describe competencies considered important for effective group facilitation. Inter-rater reliability was determined with two experienced psychologists who used the checklist to rate the competencies of psychology trainees facilitating a memory skills group. RESULTS After two Delphi rounds, consensus was reached on 17 items, defined as at least 80% agreement among the panel of 15 experts. The four checklist item categories were (a) Facilitating focused group discussion, (b) Communication skills, (c) Interpersonal style, and (d) Session structure. One item was removed after piloting. Inter-rater reliability was excellent (88% agreement) using a simple coding method (competent/incompetent). When using a detailed coding method that discriminated between 'done adequately' and 'done well', inter-rater reliability was weaker (κ = 0.481, 55% agreement); however, it improved to almost perfect after the raters calibrated their standards. CONCLUSION The new group facilitation competency checklist is fit for purpose for measuring clinical competencies in delivering group-based rehabilitation interventions and can be used in the training of effective group facilitators.", "gemini": "MỤC TIÊU Các định dạng can thiệp theo nhóm rất phổ biến trong phục hồi chức năng, nhưng hiện tại không có công cụ nào để đo lường khách quan năng lực lâm sàng trong việc điều phối nhóm. Chúng tôi nhằm mục đích phát triển một danh sách kiểm tra năng lực điều phối nhóm có tính trắc nghiệm tốt để sử dụng trong các thiết lập lâm sàng, đào tạo và nghiên cứu. PHƯƠNG PHÁP Phương pháp Delphi về sự đồng thuận của chuyên gia đã được sử dụng để thiết lập các mục danh sách kiểm tra mô tả rõ ràng các năng lực được coi là quan trọng để điều phối nhóm hiệu quả. Độ tin cậy giữa các đánh giá được xác định với hai nhà tâm lý học giàu kinh nghiệm đã sử dụng danh sách kiểm tra để đánh giá năng lực của các học viên tâm lý học trong việc điều phối nhóm kỹ năng ghi nhớ. KẾT QUẢ Sau hai vòng Delphi, đã đạt được sự đồng thuận về 17 mục, được định nghĩa là ít nhất 80% sự đồng ý trong số hội đồng gồm 15 chuyên gia. Bốn danh mục mục danh sách kiểm tra là (a) Tạo điều kiện thảo luận nhóm tập trung, (b) Kỹ năng giao tiếp, (c) Phong cách giao tiếp giữa các cá nhân và (d) Cấu trúc phiên họp. Một mục đã bị xóa sau khi thí điểm. Độ tin cậy giữa các đánh giá là tuyệt vời (88% sự đồng ý) khi sử dụng phương pháp mã hóa đơn giản (có năng lực/không có năng lực). Khi sử dụng phương pháp mã hóa chi tiết phân biệt giữa 'thực hiện đầy đủ' và 'thực hiện tốt', độ tin cậy giữa các đánh giá yếu hơn (κ = 0,481, 55% sự đồng ý); tuy nhiên, nó đã được cải thiện gần như hoàn hảo sau khi những người đánh giá hiệu chuẩn các tiêu chuẩn của họ. KẾT LUẬN Danh sách kiểm tra năng lực điều phối nhóm mới phù hợp với mục đích đo lường năng lực lâm sàng trong việc cung cấp các can thiệp phục hồi chức năng theo nhóm và có thể được sử dụng trong đào tạo các điều phối viên nhóm hiệu quả."} +{"idx": 1890131, "en": "Studies from English-speaking countries show that sex toy use is common in various populations and often associated with sexual well-being. Empirical data on sex toy use and perceived effects in other countries is still missing. Based on the Positive Sexuality framework and the Positive Technology framework, this study documented the prevalence of sex toy use in solo and partnered sex among heterosexual-identified women and men in Germany, as well as perceived positive and/or negative effects of sex toy use on sexual well-being. A survey was conducted with a national online sample of 1,723 heterosexual-identified adults in Germany (Mage = 42.71, SD = 13.25, 49% women, 51% men). The majority (52%) reported sex toy use in partnered sex, and 45% reported sex toy use in solo sex. Stronger positive than negative perceived effects of sex toy use were reported by women and men alike. Findings indicate that professionals in sexual health/sexuality education should consider sex toy use as a common and beneficial sexual behavior. Future studies could explore specific effects of sex toy use and their predictors in more detail.", "gemini": "Các nghiên cứu từ các quốc gia nói tiếng Anh cho thấy việc sử dụng đồ chơi tình dục rất phổ biến ở nhiều nhóm dân cư khác nhau và thường liên quan đến sức khỏe tình dục. Dữ liệu thực nghiệm về việc sử dụng đồ chơi tình dục và những tác động được nhận thức ở các quốc gia khác vẫn còn thiếu. Dựa trên khuôn khổ Tình dục tích cực và khuôn khổ Công nghệ tích cực, nghiên cứu này ghi nhận tỷ lệ sử dụng đồ chơi tình dục trong quan hệ tình dục một mình và có bạn tình ở những phụ nữ và nam giới tự nhận là dị tính ở Đức, cũng như những tác động tích cực và/hoặc tiêu cực được nhận thức của việc sử dụng đồ chơi tình dục đối với sức khỏe tình dục. Một cuộc khảo sát đã được tiến hành với mẫu trực tuyến toàn quốc gồm 1.723 người lớn tự nhận là dị tính ở Đức (Mage = 42,71, SD = 13,25, 49% phụ nữ, 51% nam giới). Phần lớn (52%) báo cáo sử dụng đồ chơi tình dục trong quan hệ tình dục có bạn tình và 45% báo cáo sử dụng đồ chơi tình dục trong quan hệ tình dục một mình. Phụ nữ và nam giới đều báo cáo những tác động tích cực được nhận thức mạnh mẽ hơn so với những tác động tiêu cực của việc sử dụng đồ chơi tình dục. Những phát hiện chỉ ra rằng những người làm công tác chuyên môn về sức khỏe tình dục/giáo dục giới tính nên coi việc sử dụng đồ chơi tình dục là một hành vi tình dục phổ biến và có lợi. Các nghiên cứu trong tương lai có thể khám phá chi tiết hơn các tác động cụ thể của việc sử dụng đồ chơi tình dục và các yếu tố dự báo của chúng."} +{"idx": 1890132, "en": "The prevalence of type 2 diabetes mellitus (T2DM) has been increasing throughout the world. The cereals, as the high carbohydrate food and dominant portion of diet, have crucial impacts on glycemic control, especially for T2DM. Both components in whole cereals and processing are closely related to their glycemic response. The consumption of whole cereals is shown to reduce the risk of T2DM. The starch characteristic of cereal determines its hydrolysis rate and glycemic response. The soluble and insoluble dietary fiber, phenolic compounds, and other bioactive constituents may slow down the starch hydrolysis. Besides, they have other physiological mechanisms in regulation of T2DM, such as amelioration of lipid disorder, antioxidant, anti-inflammation, and regulation of gut microbiota, which contribute to further improvement of metabolic symptoms. Cereals are subjected to processing before consumption, which is involved in mechanical force, bioprocessing, thermal treatment, and cooling. The processing induces changes in nutritional composition and physical structure compared to the raw kernels. The key influences of processing on glycemic response are the starch gelatinization and starch retrogradation. However, physical structure of cereal and interactions among starch and other compounds greatly contribute to various glycemic responses of cereal products. This review highlights recent findings on the influences of both bioactive constituents and processing on the antidiabetic effects and physiological properties of cereals.", "gemini": "Tỷ lệ mắc bệnh đái tháo đường týp 2 (T2DM) đã tăng trên toàn thế giới. Ngũ cốc, là thực phẩm có hàm lượng carbohydrate cao và là phần chủ yếu trong chế độ ăn uống, có tác động rất lớn đến việc kiểm soát đường huyết, đặc biệt là đối với T2DM. Cả thành phần trong ngũ cốc nguyên hạt và quá trình chế biến đều có liên quan chặt chẽ đến phản ứng đường huyết của chúng. Việc tiêu thụ ngũ cốc nguyên hạt được chứng minh là làm giảm nguy cơ mắc T2DM. Đặc tính tinh bột của ngũ cốc quyết định tốc độ thủy phân và phản ứng đường huyết của nó. Chất xơ hòa tan và không hòa tan, hợp chất phenolic và các thành phần hoạt tính sinh học khác có thể làm chậm quá trình thủy phân tinh bột. Bên cạnh đó, chúng còn có các cơ chế sinh lý khác trong điều hòa T2DM, chẳng hạn như cải thiện rối loạn lipid, chống oxy hóa, chống viêm và điều hòa hệ vi khuẩn đường ruột, góp phần cải thiện thêm các triệu chứng chuyển hóa. Ngũ cốc phải trải qua quá trình chế biến trước khi tiêu thụ, bao gồm lực cơ học, sinh học, xử lý nhiệt và làm mát. Quá trình chế biến gây ra những thay đổi về thành phần dinh dưỡng và cấu trúc vật lý so với hạt thô. Những ảnh hưởng chính của quá trình chế biến đối với phản ứng đường huyết là quá trình gelatin hóa tinh bột và thoái hóa tinh bột. Tuy nhiên, cấu trúc vật lý của ngũ cốc và các tương tác giữa tinh bột và các hợp chất khác đóng góp rất lớn vào các phản ứng đường huyết khác nhau của các sản phẩm ngũ cốc. Tổng quan này nêu bật những phát hiện gần đây về ảnh hưởng của cả các thành phần hoạt tính sinh học và quá trình chế biến đối với tác dụng chống đái tháo đường và các đặc tính sinh lý của ngũ cốc."} +{"idx": 1890133, "en": "A high proportion of adults who sustain a concussion identify changes in their sleep during the acute stage, typically reporting an increased need for sleep or nonrestful sleep. Our understanding of sleep following concussion is less well understood within a pediatric population. In this study, we investigated the trajectory of sleep and daytime sleepiness in a prospective cohort of 40 children and youth (6-18 years old) with concussion, 40 age-and sex-matched healthy children and youth, and 40 with upper-extremity orthopedic injury. Evaluations occurred during the acute stage (<2 weeks) and at 3-, 6-, and 12-month postinjury using the Sleep Disturbance Scale for Children and the Postconcussion Symptom Scale. There were no significant differences within- or between-group differences in sleep across all four time points with analysis of the groups as a whole. When groups were divided by age (6-11 and 12- < 18 years), there was a significant difference in the ability to initiate sleep for the younger concussed group during the acute stage, compared with healthy controls, as well as significantly greater daytime nap duration that decreased over time. Significant correlations were also found between the frequency and duration of daytime naps and Postconcussion Symptom Scale total score and subscores (cognitive, physical/migraine, mood, and sleep) in the concussed group during the acute stage. Our results suggest that in a group with noncomplicated concussion, children and youth have transient alterations in daytime sleepiness that are related to concussion symptoms. Younger children may be more vulnerable to disturbances in sleep and daytime wakefulness.", "gemini": "Tỷ lệ người lớn bị chấn động não cao nhận thấy những thay đổi về giấc ngủ của họ trong giai đoạn cấp tính, thường báo cáo rằng nhu cầu ngủ tăng hoặc giấc ngủ không ngon. Sự hiểu biết của chúng ta về giấc ngủ sau chấn động não ít được hiểu rõ hơn trong quần thể nhi khoa. Trong nghiên cứu này, chúng tôi đã điều tra quỹ đạo của giấc ngủ và buồn ngủ ban ngày trong một nhóm đối chứng gồm 40 trẻ em và thanh thiếu niên (6-18 tuổi) bị chấn động não, 40 trẻ em và thanh thiếu niên khỏe mạnh cùng tuổi và giới tính và 40 trẻ bị chấn thương chỉnh hình ở chi trên. Đánh giá được thực hiện trong giai đoạn cấp tính (<2 tuần) và vào thời điểm sau chấn thương 3, 6 và 12 tháng bằng cách sử dụng Thang đo rối loạn giấc ngủ cho trẻ em và Thang đo triệu chứng sau chấn động não. Không có sự khác biệt đáng kể nào trong hoặc giữa các nhóm về giấc ngủ trong cả bốn thời điểm với phân tích các nhóm nói chung. Khi các nhóm được chia theo độ tuổi (6-11 và 12- < 18 tuổi), có sự khác biệt đáng kể về khả năng bắt đầu ngủ ở nhóm trẻ bị chấn động não trong giai đoạn cấp tính, so với nhóm đối chứng khỏe mạnh, cũng như thời gian ngủ trưa ban ngày dài hơn đáng kể và giảm dần theo thời gian. Cũng tìm thấy mối tương quan đáng kể giữa tần suất và thời gian ngủ trưa ban ngày và tổng điểm Thang đo triệu chứng sau chấn động não và các điểm phụ (nhận thức, thể chất/đau nửa đầu, tâm trạng và giấc ngủ) ở nhóm bị chấn động não trong giai đoạn cấp tính. Kết quả của chúng tôi cho thấy rằng trong một nhóm bị chấn động não không biến chứng, trẻ em và thanh thiếu niên có những thay đổi tạm thời về buồn ngủ ban ngày có liên quan đến các triệu chứng chấn động não. Trẻ nhỏ có thể dễ bị rối loạn giấc ngủ và tỉnh táo ban ngày hơn."} +{"idx": 1890134, "en": "Objective: To investigate whether the rates of transient and enduring distress differ between general and pregnancy-specific anxiety in antenatal English-speaking women. Background: Evidence indicates that half of women with high levels of general anxiety during pregnancy will no longer be highly anxious after a few weeks, without having received treatment. Pregnancy-specific anxiety, however, may be more enduring, as many worries concerning the forthcoming birth, whether the baby will be healthy and the woman's ability to care for a newborn are likely to continue, or increase, during the pregnancy. Method: Women attending a public hospital antenatal clinic completed several mood questionnaires, including a mix of general anxiety and pregnancy-specific anxiety scales (T1). Between 2 and weeks later (T2), still during pregnancy, participants completed the same measures via a phone interview. Results: Between 76 and 243 women completed the different measures at both time points. For each measure the result was similar: about half of women scoring high at the first assessment (T1) continued to score high at T2 on both the general and pregnancy-specific anxiety measures, despite not receiving any specialist intervention. By contrast, over 90% of women initially scoring low on the various measures continued to score low at T2. Conclusion: Whether women are screened for pregnancy-specific or for general anxiety, around half scoring 'high' on the measure will no longer be in the anxious range a few weeks later. They thus have 'transient' anxiety. This transient/enduring finding was also confirmed for those with high levels of depressive symptomatology.", "gemini": "Mục tiêu: Điều tra xem tỷ lệ đau khổ tạm thời và kéo dài có khác nhau giữa lo lắng chung và lo lắng riêng về thai kỳ ở phụ nữ nói tiếng Anh trước khi sinh hay không. Bối cảnh: Bằng chứng chỉ ra rằng một nửa số phụ nữ có mức độ lo lắng chung cao trong thời kỳ mang thai sẽ không còn lo lắng cao độ sau vài tuần mà không cần điều trị. Tuy nhiên, lo lắng riêng về thai kỳ có thể kéo dài hơn, vì nhiều lo lắng liên quan đến việc sinh nở sắp tới, liệu em bé có khỏe mạnh và khả năng chăm sóc trẻ sơ sinh của người phụ nữ có khả năng tiếp tục hoặc tăng lên trong suốt thai kỳ. Phương pháp: Những phụ nữ đến phòng khám trước sinh của bệnh viện công đã hoàn thành một số bảng câu hỏi về tâm trạng, bao gồm hỗn hợp các thang đo lo lắng chung và lo lắng riêng về thai kỳ (T1). Từ 2 đến tuần sau (T2), vẫn trong thời kỳ mang thai, những người tham gia đã hoàn thành các biện pháp tương tự thông qua phỏng vấn qua điện thoại. Kết quả: Từ 76 đến 243 phụ nữ đã hoàn thành các biện pháp khác nhau tại cả hai thời điểm. Đối với mỗi biện pháp, kết quả đều tương tự: khoảng một nửa số phụ nữ có điểm cao ở lần đánh giá đầu tiên (T1) tiếp tục đạt điểm cao ở T2 đối với cả các biện pháp lo lắng chung và lo lắng riêng về thai kỳ, mặc dù không nhận được bất kỳ sự can thiệp chuyên khoa nào. Ngược lại, hơn 90% phụ nữ ban đầu đạt điểm thấp ở các biện pháp khác nhau thì tiếp tục đạt điểm thấp ở T2. Kết luận: Cho dù phụ nữ được sàng lọc về lo lắng riêng về thai kỳ hay lo lắng chung, thì khoảng một nửa số người đạt điểm 'cao' ở biện pháp này sẽ không còn trong phạm vi lo lắng sau vài tuần nữa. Do đó, họ có sự lo lắng 'tạm thời'. Phát hiện tạm thời/kéo dài này cũng được xác nhận đối với những người có mức độ triệu chứng trầm cảm cao."} +{"idx": 1890135, "en": "A method for identifying mechanical properties of arterial tissue in vivo is proposed in this paper and it is numerically validated for the human abdominal aorta. Supplied with pressure-radius data, the method determines six parameters representing relevant mechanical properties of an artery. In order to validate the method, 22 finite element arteries are created using published data for the human abdominal aorta. With these in silico abdominal aortas, which serve as mock experiments with exactly known material properties and boundary conditions, pressure-radius data sets are generated and the mechanical properties are identified using the proposed parameter identification method. By comparing the identified and pre-defined parameters, the method is quantitatively validated. For healthy abdominal aortas, the parameters show good agreement for the material constant associated with elastin and the radius of the stress-free state over a large range of values. Slightly larger discrepancies occur for the material constants associated with collagen, and the largest relative difference is obtained for the in situ axial prestretch. For pathological abdominal aortas incorrect parameters are identified, but the identification method reveals the presence of diseased aortas. The numerical validation indicates that the proposed parameter identification method is able to identify adequate parameters for healthy abdominal aortas and reveals pathological aortas from in vivo-like data.", "gemini": "Một phương pháp xác định các đặc tính cơ học của mô động mạch trong cơ thể sống được đề xuất trong bài báo này và phương pháp này được xác thực bằng số đối với động mạch chủ bụng của người. Được cung cấp dữ liệu bán kính áp suất, phương pháp xác định sáu thông số đại diện cho các đặc tính cơ học có liên quan của một động mạch. Để xác thực phương pháp, 22 động mạch phần tử hữu hạn được tạo ra bằng cách sử dụng dữ liệu đã công bố đối với động mạch chủ bụng của người. Với các động mạch chủ bụng trong silico này, đóng vai trò như các thí nghiệm giả với các đặc tính vật liệu và điều kiện biên được biết chính xác, các tập dữ liệu bán kính áp suất được tạo ra và các đặc tính cơ học được xác định bằng cách sử dụng phương pháp xác định thông số được đề xuất. Bằng cách so sánh các thông số đã xác định và được xác định trước, phương pháp này được xác thực về mặt định lượng. Đối với các động mạch chủ bụng khỏe mạnh, các thông số cho thấy sự nhất quán tốt đối với hằng số vật liệu liên quan đến elastin và bán kính của trạng thái không ứng suất trên một phạm vi giá trị lớn. Những sai lệch lớn hơn một chút xảy ra đối với các hằng số vật liệu liên quan đến collagen và sự khác biệt tương đối lớn nhất thu được đối với ứng suất trước trục tại chỗ. Đối với các động mạch chủ bụng bệnh lý, các thông số không chính xác được xác định, nhưng phương pháp xác định cho thấy sự hiện diện của các động mạch bị bệnh. Xác thực bằng số chỉ ra rằng phương pháp xác định thông số được đề xuất có khả năng xác định các thông số đầy đủ cho các động mạch chủ bụng khỏe mạnh và phát hiện các động mạch bệnh lý từ dữ liệu giống như trong cơ thể sống."} +{"idx": 1890136, "en": "There is extensive literature linking motor activity to cognitive effects at various stages in life, promoting both development and the reduction of aging associated pathologies. It is unclear whether the benefits of this activity on the cognitive level are associated with brain functions that are necessary for their performance or recurrence of activity or type of activity itself. The aim of this study was to evaluate whether the type of motor activity (fine, gross, and motor imagery) in two modes (novel and routine) can affect cognitive functions such as attention, executive functions, and praxis in college students. A 2 × 3 factorial design with repeated measures was used without a control group and pre- and post-training evaluation. Fifty-three young people (14 men and 39 women) participated, with mean age of 18.94 years (SD = 1.61 years) and were divided into six groups. Each of the groups performed relevant training 20 minutes per day for five days depending on the group. Measures were taken pre and post-training for attention tests, attention span, working memory, visual constructive skills, procedural memory, and motor skills. The results show a \"learning effect\" from the exposure to the tests in measurements after training. It was also found that between groups, there is a difference in some of the variables of procedural memory (number of errors) and working memory. More extensive training could better reflect the effects of the training, and longitudinal evaluation could show the rate of change of functions. The main clinical implication could be the evaluation of training programs for recovery and motor training in cerebral plasticity having effect on the cognitive aspects.", "gemini": "Có nhiều tài liệu liên kết hoạt động vận động với các hiệu ứng nhận thức ở nhiều giai đoạn khác nhau trong cuộc sống, thúc đẩy cả sự phát triển và giảm các bệnh lý liên quan đến lão hóa. Không rõ liệu những lợi ích của hoạt động này ở cấp độ nhận thức có liên quan đến các chức năng não cần thiết cho hoạt động hoặc sự tái diễn của hoạt động hay chính loại hoạt động đó hay không. Mục đích của nghiên cứu này là đánh giá xem loại hoạt động vận động (tinh, thô và hình ảnh vận động) ở hai chế độ (mới và thường lệ) có thể ảnh hưởng đến các chức năng nhận thức như sự chú ý, chức năng điều hành và thực hành ở sinh viên đại học hay không. Một thiết kế giai thừa 2 × 3 với các phép đo lặp lại đã được sử dụng mà không có nhóm đối chứng và đánh giá trước và sau khi đào tạo. Năm mươi ba thanh niên (14 nam và 39 nữ) đã tham gia, với độ tuổi trung bình là 18,94 tuổi (SD = 1,61 tuổi) và được chia thành sáu nhóm. Mỗi nhóm thực hiện đào tạo có liên quan 20 phút mỗi ngày trong năm ngày tùy thuộc vào nhóm. Các phép đo được thực hiện trước và sau khi đào tạo đối với các bài kiểm tra chú ý, khả năng tập trung, trí nhớ làm việc, kỹ năng xây dựng thị giác, trí nhớ thủ tục và kỹ năng vận động. Kết quả cho thấy một \"hiệu ứng học tập\" từ việc tiếp xúc với các bài kiểm tra trong các phép đo sau khi đào tạo. Người ta cũng thấy rằng giữa các nhóm, có sự khác biệt về một số biến của trí nhớ thủ tục (số lỗi) và trí nhớ làm việc. Đào tạo mở rộng hơn có thể phản ánh tốt hơn các hiệu ứng của đào tạo và đánh giá theo chiều dọc có thể cho thấy tốc độ thay đổi của các chức năng. Ý nghĩa lâm sàng chính có thể là đánh giá các chương trình đào tạo phục hồi và đào tạo vận động trong tính dẻo của não có tác động đến các khía cạnh nhận thức."} +{"idx": 1890137, "en": "Tumors that originate from the epithelium of the odontogenic apparatus are classified as benign or malignant. The proliferative activity could provide a basis for differences in the biologic behavior among the histological variants of ameloblastoma (AM) and keratocystic odontogenic tumor (KCOT). We examined 32 solid AM and 18 KCOT cases. The AM sample comprised 16 cases of follicular AM, six cases of unicystic AM, eight cases of plexiform AM and two cases of acanthomatous AM. Sections were stained with the Ki-67 antibody. Ten representative fields were selected randomly in each section. For AM, peripheral tall columnar cells of tumor islands/nests/cords were selected. For KCOT, fields were selected in the basal and the suprabasal region of the epithelial lining. We counted the average number of Ki-67 positive cells/field for AM and KCOT. AM exhibited Ki-67 expression in peripheral tall columnar cells, whereas KCOT exhibited Ki-67 expression in the basal and suprabasal layer. No significant difference between AM and KCOT was observed; the cellular proliferative activity varied among the subtypes. No significant difference in Ki-67 expression in acanthomatous, cystic and follicular types of AM was observed, although the plexiform type exhibited significantly higher levels than the other three types. High expression of Ki-67 could be a useful prognostic marker for proliferative activity and a prognostic indicator for recurrence rate of AM and KCOT.", "gemini": "Các khối u có nguồn gốc từ biểu mô của bộ máy tạo ngà được phân loại là lành tính hoặc ác tính. Hoạt động tăng sinh có thể cung cấp cơ sở cho sự khác biệt về hành vi sinh học giữa các biến thể mô học của u nguyên bào men răng (AM) và u tạo ngà nang nang sừng hóa (KCOT). Chúng tôi đã kiểm tra 32 trường hợp AM rắn và 18 trường hợp KCOT. Mẫu AM bao gồm 16 trường hợp AM nang, sáu trường hợp AM đơn nang, tám trường hợp AM đám rối và hai trường hợp AM gai. Các phần được nhuộm bằng kháng thể Ki-67. Mười trường đại diện được chọn ngẫu nhiên trong mỗi phần. Đối với AM, các tế bào hình trụ cao ngoại vi của các đảo/ổ/dây u được chọn. Đối với KCOT, các trường được chọn ở vùng đáy và vùng trên đáy của lớp biểu mô lót. Chúng tôi đếm số trung bình các tế bào dương tính với Ki-67/trường đối với AM và KCOT. AM biểu hiện Ki-67 ở các tế bào hình trụ cao ngoại vi, trong khi KCOT biểu hiện Ki-67 ở lớp đáy và lớp trên đáy. Không quan sát thấy sự khác biệt đáng kể giữa AM và KCOT; hoạt động tăng sinh của tế bào thay đổi giữa các phân nhóm. Không quan sát thấy sự khác biệt đáng kể về biểu hiện Ki-67 ở các loại AM gai, nang và nang, mặc dù loại đám rối biểu hiện mức cao hơn đáng kể so với ba loại còn lại. Biểu hiện cao Ki-67 có thể là một dấu hiệu tiên lượng hữu ích cho hoạt động tăng sinh và là một chỉ báo tiên lượng cho tỷ lệ tái phát của AM và KCOT."} +{"idx": 1890138, "en": "Drawing on an ethnographic study of reproduction in Israel, in this article I demonstrate how Orthodox Jews delineate borders between the godly and the human in their daily reproductive practices. Exploring the multiple ways access to technology affects religious belief and observance, I describe three approaches to marital birth control, two of which are antithetical: steadfast resistance to and general acceptance of \"calculated family planning.\" Seeking a middle road, the third model, \"flexible decision-making,\" reveals how couples push off and welcome pregnancies simultaneously. Unravelling the illusion of a binary model of planned/unplanned parenthood, I call for nuanced models of reproductive decision-making.", "gemini": "Dựa trên một nghiên cứu dân tộc học về sinh sản ở Israel, trong bài viết này, tôi chứng minh cách người Do Thái Chính thống vạch ra ranh giới giữa thần thánh và con người trong các hoạt động sinh sản hàng ngày của họ. Khám phá nhiều cách tiếp cận công nghệ ảnh hưởng đến niềm tin và sự tuân thủ tôn giáo, tôi mô tả ba cách tiếp cận kiểm soát sinh đẻ khi kết hôn, hai trong số đó là đối lập: kiên quyết phản đối và chấp nhận chung \"kế hoạch hóa gia đình có tính toán\". Tìm kiếm một con đường trung dung, mô hình thứ ba, \"ra quyết định linh hoạt\", tiết lộ cách các cặp vợ chồng đẩy lùi và chào đón thai kỳ cùng lúc. Làm sáng tỏ ảo tưởng về mô hình nhị phân của cha mẹ có kế hoạch/không có kế hoạch, tôi kêu gọi các mô hình sắc thái về việc ra quyết định sinh sản."} +{"idx": 1890139, "en": "Apoptosis is a fundamental process controlling normal tissue homeostasis by regulating a balance between cell proliferation and death. Predicting the subcellular location of apoptosis proteins is very helpful for understanding the mechanism of programmed cell death. Predicting protein subcellular localization with bioinformatic techniques provides quite a few opportunities in related fields. In this work, we propose the use of a hierarchical extreme learning machine (H-ELM) to make a classification of high-dimensional input data without demanding a dimension reduction process, which yields acceptable results. An attempt is made to extract features from different perspectives, and a feature fusion process is accomplished. Regarding the position-specific scoring matrix, the first type depicts the correlation within the sequence with the autocorrelation function for relatively random sections from the sequence; and the second type is the Kullback-Leibler (K-L) divergence of the two distributions formed by the amino acids' constitutuent proportions. It is illustrated in an experiment with features from different sources mixed by simple concatenation yielding a poor result, but the synthetical feature fused with stochastic nonlinear embedding (t-SNE) greatly improved the classification. Finally, the highest overall accuracy of ZD98 is 87.5% by adjusting the hyper-parameters of H-ELM, and of CL317 is 92.4%.", "gemini": "Chết rụng tế bào là một quá trình cơ bản kiểm soát cân bằng nội môi mô bình thường bằng cách điều chỉnh sự cân bằng giữa sự tăng sinh và chết của tế bào. Dự đoán vị trí dưới tế bào của các protein chết rụng tế bào rất hữu ích để hiểu cơ chế chết tế bào theo chương trình. Dự đoán vị trí dưới tế bào của protein bằng các kỹ thuật tin sinh học cung cấp khá nhiều cơ hội trong các lĩnh vực liên quan. Trong công trình này, chúng tôi đề xuất sử dụng máy học cực đoan phân cấp (H-ELM) để phân loại dữ liệu đầu vào có chiều cao mà không yêu cầu quá trình giảm chiều, mang lại kết quả chấp nhận được. Người ta đã cố gắng trích xuất các đặc điểm từ các góc độ khác nhau và thực hiện một quá trình hợp nhất các đặc điểm. Về ma trận chấm điểm vị trí cụ thể, loại đầu tiên mô tả mối tương quan trong trình tự với hàm tự tương quan cho các phần tương đối ngẫu nhiên từ trình tự; và loại thứ hai là độ phân kỳ Kullback-Leibler (K-L) của hai phân phối được hình thành bởi tỷ lệ thành phần của các axit amin. Nó được minh họa trong một thí nghiệm với các đặc điểm từ các nguồn khác nhau được trộn bằng cách nối đơn giản cho kết quả kém, nhưng đặc điểm tổng hợp được hợp nhất với nhúng phi tuyến ngẫu nhiên (t-SNE) đã cải thiện đáng kể phân loại. Cuối cùng, độ chính xác tổng thể cao nhất của ZD98 là 87,5% bằng cách điều chỉnh các siêu tham số của H-ELM và của CL317 là 92,4%."} +{"idx": 1890140, "en": "Drive hunts are a method to herd, capture and kill small cetaceans (whales and dolphins) in coastal waters of some countries including Japan and the Faroe Islands. In Japan, these methods are often associated with the acquisition of live dolphins for international marine parks and aquaria. During the hunts, dolphins are herded by a flotilla of fishing vessels and loud underwater noise created by fishermen banging hammers on metal poles. The prolonged and strenuous chase and use of sound barriers to herd, capture, and restrain the dolphins can result in acute stress and injury. The authors review physiological and behavioral data pertaining to chase, encirclement, and live capture of dolphins and draw comparisons between chase and capture data for marine and terrestrial species. This analysis raises substantial welfare concerns associated with the hunts and acquisition of dolphins from such capture operations. The authors assert that this data detailing the negative impacts of chase, herding and handling (capture) of small cetaceans renders these hunts inherently inhumane and should inform policy relating to the collection and management of dolphins in the wild.", "gemini": "Săn đuổi là một phương pháp chăn thả, bắt và giết các loài động vật có vú nhỏ (cá voi và cá heo) ở vùng nước ven biển của một số quốc gia bao gồm Nhật Bản và Quần đảo Faroe. Ở Nhật Bản, các phương pháp này thường gắn liền với việc mua cá heo sống cho các công viên và thủy cung biển quốc tế. Trong quá trình săn bắt, cá heo được một đội tàu đánh cá và tiếng ồn lớn dưới nước do ngư dân đập búa vào các cọc kim loại chăn thả. Cuộc rượt đuổi kéo dài và vất vả cùng việc sử dụng các rào cản âm thanh để chăn thả, bắt và giữ cá heo có thể gây ra căng thẳng và thương tích cấp tính. Các tác giả xem xét dữ liệu về sinh lý và hành vi liên quan đến việc rượt đuổi, bao vây và bắt sống cá heo và so sánh dữ liệu rượt đuổi và bắt giữa các loài sinh vật biển và trên cạn. Phân tích này nêu lên những lo ngại đáng kể về phúc lợi liên quan đến việc săn bắt và mua cá heo từ các hoạt động đánh bắt như vậy. Các tác giả khẳng định rằng dữ liệu này nêu chi tiết các tác động tiêu cực của việc rượt đuổi, chăn thả và xử lý (bắt) các loài động vật có vú nhỏ khiến những cuộc săn bắt này vốn đã vô nhân đạo và cần phải thông báo cho chính sách liên quan đến việc thu thập và quản lý cá heo trong tự nhiên."} +{"idx": 1890141, "en": "Recovery from mild traumatic brain injury (mTBI) and regaining emotional equilibrium afterward can take much longer than the typical three months. Recent works attribute persisting complaints to psychological factors, primarily the negative perception of mTBI. However, research has yet to demonstrate how self-beliefs concerning capability are linked to perception and ability to accept injury. The objective of this study was to investigate how perceived general self-efficacy (GSE) and acceptance of disability (AD) relate to emotional outcome following mTBI. Thirty individuals aged 21-57, all of whom were at least three-month post diagnosis of mTBI, underwent a psychiatric clinical interview assessing depression, post-traumatic stress disorder (PTSD), and quality of life (QoL), and completed self-report scales to evaluate psychological factors. Scores on AD, depression, and QoL for most life domains were significantly lower and worse than among the normative population. Pearson coefficients indicated significant correlations between psychological factors and emotional outcome. Mediation analysis showed a significant role of AD in mediating the correlation between GSE and depression/general QoL, irrespective of PTSD. Low self-efficacy accentuates negative perception of the injury which, in turn, leads to poor emotional outcomes post mTBI. Therefore, AD should become the focus of therapeutic interventions.", "gemini": "Quá trình phục hồi sau chấn thương sọ não nhẹ (mTBI) và lấy lại cân bằng cảm xúc sau đó có thể lâu hơn nhiều so với thời gian ba tháng thông thường. Các công trình gần đây cho rằng các khiếu nại dai dẳng là do các yếu tố tâm lý, chủ yếu là nhận thức tiêu cực về mTBI. Tuy nhiên, nghiên cứu vẫn chưa chứng minh được cách thức các niềm tin của bản thân liên quan đến khả năng nhận thức và khả năng chấp nhận chấn thương. Mục tiêu của nghiên cứu này là điều tra cách thức mà hiệu quả chung được nhận thức (GSE) và sự chấp nhận khuyết tật (AD) liên quan đến kết quả cảm xúc sau mTBI. Ba mươi cá nhân trong độ tuổi từ 21 đến 57, tất cả đều được chẩn đoán mTBI ít nhất ba tháng, đã trải qua một cuộc phỏng vấn lâm sàng về tâm thần để đánh giá tình trạng trầm cảm, rối loạn căng thẳng sau chấn thương (PTSD) và chất lượng cuộc sống (QoL) và hoàn thành thang tự báo cáo để đánh giá các yếu tố tâm lý. Điểm số về AD, trầm cảm và QoL đối với hầu hết các lĩnh vực của cuộc sống đều thấp hơn và tệ hơn đáng kể so với dân số chuẩn. Các hệ số Pearson chỉ ra mối tương quan đáng kể giữa các yếu tố tâm lý và kết quả cảm xúc. Phân tích trung gian cho thấy vai trò quan trọng của AD trong việc trung gian mối tương quan giữa GSE và trầm cảm/QoL chung, không phụ thuộc vào PTSD. Hiệu quả bản thân thấp làm tăng nhận thức tiêu cực về chấn thương, từ đó dẫn đến kết quả cảm xúc kém sau mTBI. Do đó, AD nên trở thành trọng tâm của các biện pháp can thiệp điều trị."} +{"idx": 1890142, "en": "Objectives: The present study aimed at comparing the sleepiness curve of young men with attention-deficit hyperactivity disorder (ADHD) to that of young men without ADHD before, during, and after a night of sleep deprivation. Participants: Thirty young men (age 18-30) of whom 14 were diagnosed with ADHD combined type (ADHD-C) and 16 without ADHD. Methods: The participants' sleep was monitored for 5 days via actigraphy. Subsequently, the participants were kept continuously awake in a controlled environment for 25 hr (8:00 a.m.-9:00 a.m.). The sleepiness of the participants was assessed every hour by the Karolinska Sleepiness Scale (KSS) in order to obtain the sleepiness curve of both study groups. Results: Actigraphy data demonstrated that the two groups did not differ in their total sleep time, sleep onset latency, or sleep efficiency during the 5 nights preceding the experimental session. However, during the experimental session, the ADHD group demonstrated higher sleepiness scores, particularly following midnight and on the morning following the night of sleep deprivation. Moreover, on the morning following sleep deprivation the proportion of participants reporting extreme levels of sleepiness (KSS > 7) was significantly higher in the ADHD group than in the control group. Conclusions: Young men with ADHD suffer from sleepiness more than their counterparts from the general population, and are particularly vulnerable to the effects of sleep deprivation on sleepiness. As excessive daytime sleepiness negatively affects cognition and increases the risk for motor vehicle crash and other accidents, these findings may have important clinical implications.", "gemini": "Mục tiêu: Nghiên cứu hiện tại nhằm mục đích so sánh đường cong buồn ngủ của những người đàn ông trẻ mắc chứng rối loạn tăng động giảm chú ý (ADHD) với những người đàn ông trẻ không mắc ADHD trước, trong và sau một đêm thiếu ngủ. Người tham gia: Ba mươi người đàn ông trẻ (tuổi từ 18 đến 30), trong đó 14 người được chẩn đoán mắc ADHD loại kết hợp (ADHD-C) và 16 người không mắc ADHD. Phương pháp: Giấc ngủ của những người tham gia được theo dõi trong 5 ngày thông qua phương pháp đo hoạt động. Sau đó, những người tham gia được giữ liên tục thức trong môi trường có kiểm soát trong 25 giờ (8:00 sáng - 9:00 sáng). Cảm giác buồn ngủ của những người tham gia được đánh giá hàng giờ bằng thang đo buồn ngủ Karolinska (KSS) để có được đường cong buồn ngủ của cả hai nhóm nghiên cứu. Kết quả: Dữ liệu đo hoạt động cho thấy hai nhóm không khác nhau về tổng thời gian ngủ, thời gian bắt đầu ngủ hoặc hiệu quả ngủ trong 5 đêm trước buổi thử nghiệm. Tuy nhiên, trong buổi thử nghiệm, nhóm ADHD cho thấy điểm buồn ngủ cao hơn, đặc biệt là sau nửa đêm và vào buổi sáng sau đêm thiếu ngủ. Hơn nữa, vào buổi sáng sau khi thiếu ngủ, tỷ lệ người tham gia báo cáo mức buồn ngủ cực độ (KSS> 7) cao hơn đáng kể ở nhóm ADHD so với nhóm đối chứng. Kết luận: Những người đàn ông trẻ mắc ADHD bị buồn ngủ nhiều hơn những người cùng lứa tuổi trong dân số nói chung và đặc biệt dễ bị ảnh hưởng của việc thiếu ngủ đối với tình trạng buồn ngủ. Vì buồn ngủ quá mức vào ban ngày ảnh hưởng tiêu cực đến nhận thức và làm tăng nguy cơ tai nạn xe cơ giới và các tai nạn khác, nên những phát hiện này có thể có ý nghĩa lâm sàng quan trọng."} +{"idx": 1890143, "en": "Introduction: This case of Loperamide misuse had refractory ventricular arrhythmias and was successfully supported by VA ECMO. Loperamide is currently available without prescription and can be obtained in large quantities over the internet despite Food and Drug Administration (FDA) 2016 black box warning noting cardiac toxicity. This case illustrates the life-threatening toxicity of loperamide and suggests a supportive modality to provide clinical time while the drug is cleared endogenously or exogenously. Case report: A 36-year-old female was found minimally responsive. Vital signs and monitoring revealed wide complex bradycardia, undetectable blood pressure, hypothermia, bradypnea, and hypoglycemia. The rhythm degenerated to polymorphic ventricular tachycardia cardia refractory to multiple ACLS protocols. VA-ECMO was initiated with immediate stabilization. Subsequent history revealed massive consumption of loperamide taking 400-600 mg daily. Highest known loperamide and N-desmethyl-loperamide levels were 32 and 500 ng/ml respectively. Since loperamide and metabolites are known to be protein bound, molecular adsorbent recirculating system (MARS) was initiated for toxin clearance. Additionally, she developed acute renal failure supported by CRRT. She was ultimately weaned from ECMO, MARS, and CRRT and discharged neurologically intact on hospital day 12. Discussion: VA ECMO for hemodynamic support provided the needed time for natural resolution of the cardiac toxicity while providing adequate perfusion. MARS was used in the setting of highly protein bound toxins, but drug clearance could not be demonstrated through serial levels. VA ECMO (or referral to a center with VA ECMO) should be considered with lethal loperamide-induced cardiotoxicity and perhaps other cardio-toxins.", "gemini": "Giới thiệu: Trường hợp lạm dụng Loperamide này có loạn nhịp thất kháng trị và được hỗ trợ thành công bởi VA ECMO. Hiện tại, Loperamide không cần kê đơn và có thể mua với số lượng lớn trên internet mặc dù Cục Quản lý Thực phẩm và Dược phẩm (FDA) năm 2016 đã cảnh báo về độc tính trên tim. Trường hợp này minh họa về độc tính đe dọa tính mạng của loperamide và đề xuất một phương thức hỗ trợ để cung cấp thời gian lâm sàng trong khi thuốc được đào thải ra ngoài cơ thể hoặc ngoại sinh. Báo cáo trường hợp: Một phụ nữ 36 tuổi được phát hiện phản ứng tối thiểu. Các dấu hiệu sinh tồn và theo dõi cho thấy nhịp tim chậm phức hợp rộng, không phát hiện được huyết áp, hạ thân nhiệt, chậm thở và hạ đường huyết. Nhịp tim chuyển thành nhịp nhanh thất đa hình kháng thuốc đối với nhiều phác đồ ACLS. VA-ECMO đã được khởi xướng với sự ổn định ngay lập tức. Tiền sử sau đó cho thấy tiêu thụ loperamide rất nhiều, dùng 400-600 mg mỗi ngày. Nồng độ loperamide và N-desmethyl-loperamide cao nhất được biết đến lần lượt là 32 và 500 ng / ml. Vì loperamide và các chất chuyển hóa được biết là liên kết với protein, nên hệ thống tuần hoàn hấp phụ phân tử (MARS) đã được khởi xướng để thanh thải độc tố. Ngoài ra, cô ấy còn bị suy thận cấp được hỗ trợ bởi CRRT. Cuối cùng, cô đã cai ECMO, MARS và CRRT và xuất viện với tình trạng thần kinh còn nguyên vẹn vào ngày thứ 12 trong bệnh viện. Thảo luận: VA ECMO để hỗ trợ huyết động đã cung cấp thời gian cần thiết để giải quyết tự nhiên tình trạng ngộ độc tim trong khi vẫn cung cấp đủ tưới máu. MARS được sử dụng trong bối cảnh độc tố liên kết với protein cao, nhưng không thể chứng minh được sự thanh thải thuốc thông qua các mức độ liên tiếp. VA ECMO (hoặc chuyển đến trung tâm có VA ECMO) nên được xem xét với độc tính tim do loperamide gây tử vong và có lẽ là các độc tố tim khác."} diff --git a/retranslated_vi_pubmed/chunk_189/error.json b/retranslated_vi_pubmed/chunk_189/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_189/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_19/completed.json b/retranslated_vi_pubmed/chunk_19/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..d27b10ccff3cffa11d272eef451f5e1e071e5667 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_19/completed.json @@ -0,0 +1,9996 @@ +[ + 190000, + 190001, + 190002, + 190003, + 190004, + 190005, + 190006, + 190007, + 190008, + 190009, + 190010, + 190011, + 190012, + 190013, + 190014, + 190015, + 190016, + 190017, + 190018, + 190019, + 190020, + 190021, + 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Methods Complete ophthalmic examinations, as well as corneal esthesiometry, the phenol red thread test, intraocular pressure, and Fourier-domain optical coherence tomography were performed on Yap1 +/- and age-matched wild-type (WT) mice between eyelid opening (2 weeks after birth) and adulthood (2 months and 1 year after birth). Following euthanasia, enucleated eyes were characterized histologically. Results Microphthalmia with small palpebral fissures, corneal fibrosis, and reduced corneal sensation were common findings in the Yap1 +/- mice. Generalized corneal fibrosis precluded clinical examination of the posterior structures. Histologically, thinning and keratinization of the corneal epithelium were observed in the Yap1 +/- mice in comparison with the WT mice. Distorted collagen fiber arrangement and hypercellularity of keratocytes were observed in the stroma. Descemet's membrane was extremely thin and lacked an endothelial layer in the Yap1 +/- mice. The iris was adherent to the posterior cornea along most of its surface creating a distorted contour. Most of the Yap1 +/- eyes were microphakic with swollen fibers and bladder cells. The retinas of the Yap1 +/- mice were normal at 2 weeks and 2 months of age, but the presence of retinal abnormalities, including retinoschisis and detachment, was markedly increased in the Yap1 +/- mice at 1 year of age. Conclusions The results show that the heterozygous deletion of the Yap1 gene in mice leads to complex ocular abnormalities, including microphthalmia, corneal fibrosis, anterior segment dysgenesis, and cataract.", "gemini": "Mục đích Nhằm xác định tác động của việc xóa một bản sao của Yap1 (Yap1 +/-) ở mắt chuột, các hậu quả về kiểu hình ở mắt của Yap1 +/- đã được xác định chi tiết. Phương pháp Các xét nghiệm nhãn khoa hoàn chỉnh, cũng như đo độ nhạy giác mạc, xét nghiệm chỉ đỏ phenol, áp suất nội nhãn và chụp cắt lớp quang học miền Fourier đã được thực hiện trên chuột Yap1 +/- và chuột WT (kiểu hoang dã) cùng độ tuổi giữa thời điểm mở mí mắt (2 tuần sau khi sinh) và tuổi trưởng thành (2 tháng và 1 năm sau khi sinh). Sau khi gây tử vong, mắt đã được cắt bỏ được đặc trưng về mặt mô học. Kết quả Viêm màng bồ đào với các khe mi nhỏ, xơ hóa giác mạc và giảm cảm giác giác mạc là những phát hiện phổ biến ở chuột Yap1 +/-. Xơ giác mạc toàn thể ngăn cản việc khám lâm sàng các cấu trúc phía sau. Về mặt mô học, mỏng và sừng hóa biểu mô giác mạc đã được quan sát thấy ở chuột Yap1 +/- so với chuột WT. Sắp xếp sợi collagen bị biến dạng và tăng tế bào của tế bào sừng mạc đã được quan sát thấy ở lớp trung bì. Màng Descemet cực kỳ mỏng và thiếu lớp nội mô ở chuột Yap1 +/-. Mống mắt bám vào giác mạc sau dọc theo hầu hết bề mặt của nó tạo nên một đường viền méo mó. Hầu hết các mắt Yap1 +/- đều bị đục thủy tinh thể với các sợi bị sưng và các tế bào bàng quang. Võng mạc của chuột Yap1 +/- bình thường ở tuổi 2 tuần và 2 tháng, nhưng sự hiện diện của các bất thường về võng mạc, bao gồm cả võng mạc và bong võng mạc, tăng đáng kể ở chuột Yap1 +/- ở tuổi 1. Kết luận Kết quả cho thấy việc xóa gen Yap1 dị hợp tử ở chuột dẫn đến các bất thường phức tạp ở mắt, bao gồm cả viêm màng bồ đào, xơ giác mạc, loạn sản phân đoạn trước và đục thủy tinh thể."} +{"idx": 1900001, "en": "Purpose To identify the genetic basis for retinitis pigmentosa (RP) in a cohort of Jewish patients from Caucasia. Methods Patients underwent a detailed ophthalmic evaluation, including funduscopic examination, visual field testing, optical coherence tomography (OCT), and electrophysiological tests, electroretinography (ERG) and visual evoked potentials (VEP). Genetic analysis was performed with a combination of whole exome sequencing (WES) and Sanger sequencing. Bioinformatic analysis of the WES results was performed via a customized pipeline. Pathogenicity of the identified intronic variant was evaluated in silico using the web tool Human Splicing Finder, and in vitro, using a minigene-based splicing assay. Linkage disequilibrium (LD) analysis was used to demonstrate a founder effect, and the decay of LD over generations around the mutation in Caucasus Jewish chromosomes was modeled to estimate the age of the most recent common ancestor. Results In eight patients with RP from six unrelated families, all of Caucasus Jewish ancestry, we identified a novel homozygous intronic variant, located at position -9 of PDE6B intron 15. The c.1921-9C>G variant was predicted to generate a novel acceptor splice site, nine bases upstream of the original splice site of intron 15. In vitro splicing assay demonstrated that this novel acceptor splice site is used instead of the wild-type site, leading to an 8-bp insertion into exon 16, which is predicted to cause a frameshift. The presence of a common ancestral haplotype in mutation-bearing chromosomes was compatible with a founder effect. Conclusions The PDE6B c.1921-9C>G intronic mutation is a founder mutation that accounts for at least 40% (6/15 families) of autosomal recessive RP among Caucasus Jews. This result is highly important for molecular diagnosis, carrier screening, and genetic counseling in this population.", "gemini": "Mục đích Nhằm xác định cơ sở di truyền của bệnh võng mạc sắc tố (RP) trong một nhóm bệnh nhân Do Thái từ Caucasia. Phương pháp Bệnh nhân đã trải qua đánh giá nhãn khoa chi tiết, bao gồm khám đáy mắt, kiểm tra thị trường, chụp cắt lớp quang học (OCT) và các xét nghiệm điện sinh lý, điện đồ võng mạc (ERG) và điện thế thị giác gây ra (VEP). Phân tích di truyền được thực hiện kết hợp giữa giải trình tự toàn bộ exome (WES) và giải trình tự Sanger. Phân tích tin sinh học của kết quả WES được thực hiện thông qua một quy trình tùy chỉnh. Tính gây bệnh của biến thể intron được xác định đã được đánh giá trong silico bằng cách sử dụng công cụ web Human Splicing Finder và trong ống nghiệm, bằng cách sử dụng xét nghiệm ghép nối dựa trên gen nhỏ. Phân tích liên kết không cân bằng (LD) được sử dụng để chứng minh hiệu ứng sáng lập và sự suy giảm của LD qua nhiều thế hệ xung quanh đột biến trong nhiễm sắc thể Do Thái Caucasus được mô hình hóa để ước tính tuổi của tổ tiên chung gần đây nhất. Kết quả Ở tám bệnh nhân mắc RP từ sáu gia đình không có quan hệ họ hàng, tất cả đều có tổ tiên là người Do Thái Caucasus, chúng tôi đã xác định được một biến thể intron đồng hợp tử mới, nằm ở vị trí -9 của intron 15 PDE6B. Biến thể c.1921-9C>G được dự đoán sẽ tạo ra một vị trí ghép nối thụ thể mới, chín bazơ ngược dòng so với vị trí ghép nối ban đầu của intron 15. Xét nghiệm ghép nối trong ống nghiệm chứng minh rằng vị trí ghép nối thụ thể mới này được sử dụng thay cho vị trí kiểu hoang dã, dẫn đến chèn 8 cặp bazơ vào exon 16, được dự đoán sẽ gây ra dịch khung. Sự hiện diện của một kiểu gen tổ tiên chung trong các nhiễm sắc thể mang đột biến tương thích với hiệu ứng sáng lập. Kết luận Đột biến intron PDE6B c.1921-9C>G là một đột biến sáng lập chiếm ít nhất 40% (6/15 gia đình) bệnh RP lặn trên nhiễm sắc thể thường ở người Do Thái Caucasus. Kết quả này rất quan trọng đối với chẩn đoán phân tử, sàng lọc người mang gen và tư vấn di truyền trong quần thể này."} +{"idx": 1900002, "en": "The study is to investigate effects of andrographolide on experimental autoimmune myocarditis (EAM). Lewis rats were immunized on day 0 with porcine cardiac myosin to establish EAM. The EAM rats were treated with either andrographolide (25, 50, 100 mg/kg/day) or vehicle for 21 days. An antigen-specific splenocytes proliferation assay was performed by using the cells from control rats immunized with cardiac myosin. Survival rates, myocardial pathology and myocardial functional parameters (left ventricle end-diastolic pressure, ± dP/dt and left ventricular internal dimension) of EAM rats received andrographolide were significantly improved. Andrographolide treatment caused an decrease in the infiltration of CD3+ and CD14+ positive cells in myocardial tissue. Moreover, andrographolide treatment caused a reduction in the plasma levels of tumor necrosis factor-alpha, interleukin-17 (IL-17) and myosin-antibody, and an increase in the level of IL-10 in EAM rats. Oral administration of andrographolide resulted in the decreased expression of p-PI3K, p-Akt without any change of PI3K and Akt. Further results indicate andrographolide significantly inhibited myosin-induced proliferation in splenocytes, and this effect was inhibited by co-treatment of SC79 (Akt activator). Our data indicate andrographolide inhibits development of EAM, and this beneficial effect may be due to powerful anti-inflammatory activity and inhibitory effect on PI3K/Akt pathway.", "gemini": "Nghiên cứu nhằm điều tra tác dụng của andrographolide trên bệnh cơ tim tự miễn thực nghiệm (EAM). Chuột Lewis được tiêm chủng vào ngày 0 với myosin tim lợn để thiết lập EAM. Chuột EAM được điều trị bằng andrographolide (25, 50, 100 mg/kg/ngày) hoặc dung môi trong 21 ngày. Xét nghiệm tăng sinh tế bào lách đặc hiệu với kháng nguyên được thực hiện bằng cách sử dụng các tế bào từ chuột đối chứng được tiêm chủng với myosin tim. Tỷ lệ sống sót, bệnh lý cơ tim và các thông số chức năng cơ tim (áp suất cuối tâm trương thất trái, ± dP/dt và kích thước bên trong thất trái) của chuột EAM được dùng andrographolide được cải thiện đáng kể. Điều trị bằng andrographolide gây ra sự giảm thâm nhiễm của các tế bào dương tính với CD3+ và CD14+ trong mô cơ tim. Hơn nữa, điều trị bằng andrographolide gây ra sự giảm nồng độ yếu tố hoại tử khối u alpha, interleukin-17 (IL-17) và kháng thể myosin trong huyết tương và làm tăng nồng độ IL-10 ở chuột EAM. Việc sử dụng andrographolide bằng đường uống dẫn đến giảm biểu hiện của p-PI3K, p-Akt mà không có bất kỳ thay đổi nào của PI3K và Akt. Các kết quả tiếp theo chỉ ra rằng andrographolide ức chế đáng kể sự tăng sinh do myosin gây ra ở tế bào lách và tác dụng này bị ức chế bởi đồng điều trị SC79 (chất hoạt hóa Akt). Dữ liệu của chúng tôi chỉ ra rằng andrographolide ức chế sự phát triển của EAM và tác dụng có lợi này có thể là do hoạt động chống viêm mạnh mẽ và tác dụng ức chế trên con đường PI3K/Akt."} +{"idx": 1900003, "en": "Endoplasmic reticulum (ER) stress is mediated by disturbance of Ca2+ homeostasis. The store-operated calcium (SOC) channel is the primary Ca2+ channel in non-excitable cells, but its participation in agent-induced ER stress is not clear. In this study, the effects of tunicamycin on Ca2+ influx in human umbilical vein endothelial cells (HUVECs) were observed with the fluorescent probe Fluo-4 AM. The effect of tunicamycin on the expression of the unfolded protein response (UPR)-related proteins BiP and CHOP was assayed by western blotting with or without inhibition of Orai1. Tunicamycin induced endothelial dysfunction by activating ER stress. Orai1 expression and the influx of extracellular Ca2+ in HUVECs were both upregulated during ER stress. The SOC channel inhibitor SKF96365 reversed tunicamycin-induced endothelial cell dysfunction by inhibiting ER stress. Regulation of tunicamycin-induced ER stress by Orai1 indicates that modification of Orai1 activity may have therapeutic value for conditions with ER stress-induced endothelial dysfunction.", "gemini": "Stress nội lưới tương chất (ER) được trung gian bởi sự rối loạn cân bằng Ca2+. Kênh canxi hoạt động theo kho (SOC) là kênh Ca2+ chính trong các tế bào không hưng phấn, nhưng sự tham gia của nó vào stress ER do tác nhân gây ra vẫn chưa rõ ràng. Trong nghiên cứu này, các tác động của tunicamycin đối với dòng Ca2+ trong các tế bào nội mô tĩnh mạch rốn người (HUVEC) đã được quan sát bằng đầu dò huỳnh quang Fluo-4 AM. Tác dụng của tunicamycin đối với sự biểu hiện của các protein liên quan đến đáp ứng protein chưa gấp (UPR) BiP và CHOP đã được xác định bằng phương pháp chấm điểm kiểu Western có hoặc không có sự ức chế của Orai1. Tunicamycin gây ra rối loạn chức năng nội mô bằng cách kích hoạt stress ER. Biểu hiện Orai1 và dòng Ca2+ ngoại bào trong HUVEC đều được điều hòa tăng lên trong quá trình stress ER. Chất ức chế kênh SOC SKF96365 đảo ngược tình trạng rối loạn chức năng tế bào nội mô do tunicamycin gây ra bằng cách ức chế stress ER. Sự điều hòa stress ER do tunicamycin gây ra bởi Orai1 chỉ ra rằng việc điều chỉnh hoạt động của Orai1 có thể có giá trị điều trị đối với các tình trạng rối loạn chức năng nội mô do stress ER gây ra."} +{"idx": 1900004, "en": "Glutamate toxicity-mediated mitochondrial dysfunction and neuronal cell death are involved in the pathogenesis of several neurodegenerative diseases as well as acute brain ischemia/stroke. In this study, we investigated the neuroprotective mechanism of dieckol (DEK), one of the phlorotannins isolated from the marine brown alga Ecklonia cava, against glutamate toxicity. Primary cortical neurons (100 µM, 24 h) and HT22 neurons (5 mM, 12 h) were stimulated with glutamate to induce glutamate toxic condition. The results demonstrated that DEK treatment significantly increased cell viability in a dose-dependent manner (1-50 µM) and recovered morphological deterioration in glutamate-stimulated neurons. In addition, DEK strongly attenuated intracellular reactive oxygen species (ROS) levels, mitochondrial overload of Ca2+ and ROS, mitochondrial membrane potential (ΔΨm) disruption, adenine triphosphate depletion. DEK showed free radical scavenging activity in the cell-free system. Furthermore, DEK enhanced protein expression of heme oxygenase-1 (HO-1), an important anti-oxidant enzyme, via the nuclear translocation of nuclear factor-like 2 (Nrf2). Taken together, we conclude that DEK exerts neuroprotective activities against glutamate toxicity through its direct free radical scavenging property and the Nrf-2/HO-1 pathway activation.", "gemini": "Rối loạn chức năng ty thể do độc tính glutamate và chết tế bào thần kinh liên quan đến quá trình sinh bệnh của một số bệnh thoái hóa thần kinh cũng như thiếu máu não cục bộ cấp tính/đột quỵ. Trong nghiên cứu này, chúng tôi đã điều tra cơ chế bảo vệ thần kinh của dieckol (DEK), một trong những phlorotannin được phân lập từ tảo nâu biển Ecklonia cava, chống lại độc tính glutamate. Các tế bào thần kinh vỏ não nguyên phát (100 µM, 24 giờ) và các tế bào thần kinh HT22 (5 mM, 12 giờ) được kích thích bằng glutamate để gây ra tình trạng độc tính glutamate. Kết quả chứng minh rằng xử lý DEK làm tăng đáng kể khả năng sống của tế bào theo cách phụ thuộc vào liều (1-50 µM) và phục hồi sự suy thoái về mặt hình thái ở các tế bào thần kinh bị kích thích bởi glutamate. Ngoài ra, DEK làm giảm mạnh các mức độ phản ứng oxy hóa nội bào (ROS), quá tải Ca2+ và ROS ở ty thể, phá vỡ tiềm năng màng ty thể (ΔΨm), làm cạn kiệt adenine triphosphate. DEK cho thấy hoạt tính dọn gốc tự do trong hệ thống không có tế bào. Hơn nữa, DEK tăng cường biểu hiện protein của heme oxygenase-1 (HO-1), một enzym chống oxy hóa quan trọng, thông qua sự chuyển vị nhân của nhân tố giống như nhân tố hạt nhân 2 (Nrf2). Tổng hợp lại, chúng tôi kết luận rằng DEK phát huy hoạt động bảo vệ thần kinh chống lại độc tính glutamate thông qua đặc tính dọn gốc tự do trực tiếp và hoạt hóa con đường Nrf-2/HO-1."} +{"idx": 1900005, "en": "Manually reviewing electroencephalograms (EEGs) is labor-intensive and demands automated seizure detection systems. To construct an efficient and robust event detector for experimental seizures from continuous EEG monitoring, we combined spectral analysis and deep neural networks. A deep neural network was trained to discriminate periodograms of 5-sec EEG segments from annotated convulsive seizures and the pre- and post-EEG segments. To use the entire EEG for training, a second network was trained with non-seizure EEGs that were misclassified as seizures by the first network. By sequentially applying the dual deep neural networks and simple pre- and post-processing, our autodetector identified all seizure events in 4,272 h of test EEG traces, with only 6 false positive events, corresponding to 100% sensitivity and 98% positive predictive value. Moreover, with pre-processing to reduce the computational burden, scanning and classifying 8,977 h of training and test EEG datasets took only 2.28 h with a personal computer. These results demonstrate that combining a basic feature extractor with dual deep neural networks and rule-based pre- and post-processing can detect convulsive seizures with great accuracy and low computational burden, highlighting the feasibility of our automated seizure detection algorithm.", "gemini": "Việc xem xét thủ công các điện não đồ (EEG) đòi hỏi nhiều công sức và đòi hỏi các hệ thống phát hiện động kinh tự động. Để xây dựng một bộ phát hiện sự kiện hiệu quả và mạnh mẽ cho các cơn động kinh thực nghiệm từ quá trình theo dõi EEG liên tục, chúng tôi đã kết hợp phân tích phổ và mạng nơ-ron sâu. Một mạng nơ-ron sâu đã được đào tạo để phân biệt chu kỳ của các đoạn EEG 5 giây từ các cơn co giật có chú thích và các đoạn EEG trước và sau. Để sử dụng toàn bộ EEG để đào tạo, một mạng thứ hai đã được đào tạo với các EEG không co giật bị mạng đầu tiên phân loại sai là co giật. Bằng cách áp dụng tuần tự các mạng nơ-ron sâu kép và xử lý trước và sau đơn giản, bộ tự động phát hiện của chúng tôi đã xác định được tất cả các sự kiện co giật trong 4.272 giờ theo dõi EEG thử nghiệm, chỉ với 6 sự kiện dương tính giả, tương ứng với độ nhạy 100% và giá trị dự đoán dương tính 98%. Hơn nữa, với việc xử lý trước để giảm gánh nặng tính toán, việc quét và phân loại 8.977 giờ dữ liệu EEG đào tạo và thử nghiệm chỉ mất 2,28 giờ với máy tính cá nhân. Những kết quả này chứng minh rằng việc kết hợp trình trích xuất đặc điểm cơ bản với các mạng nơ-ron sâu kép và xử lý trước và sau dựa trên quy tắc có thể phát hiện các cơn co giật với độ chính xác cao và gánh nặng tính toán thấp, làm nổi bật tính khả thi của thuật toán phát hiện động kinh tự động của chúng tôi."} +{"idx": 1900006, "en": "Despite increased evidence of bio-activity following far-infrared (FIR) radiation, susceptibility of cell signaling to FIR radiation-induced homeostasis is poorly understood. To observe the effects of FIR radiation, FIR-radiated materials-coated fabric was put on experimental rats or applied to L6 cells, and microarray analysis, quantitative real-time polymerase chain reaction, and wound healing assays were performed. Microarray analysis revealed that messenger RNA expressions of rat muscle were stimulated by FIR radiation in a dose-dependent manner in amount of 10% and 30% materials-coated. In 30% group, 1,473 differentially expressed genes were identified (fold change [FC] > 1.5), and 218 genes were significantly regulated (FC > 1.5 and p < 0.05). Microarray analysis showed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and cell migration-related pathways were significantly stimulated in rat muscle. ECM and platelet-derived growth factor (PDGF)-mediated cell migration-related genes were increased. And, results showed that the relative gene expression of actin beta was increased. FIR radiation also stimulated actin subunit and actin-related genes. We observed that wound healing was certainly promoted by FIR radiation over 48 h in L6 cells. Therefore, we suggest that FIR radiation can penetrate the body and stimulate PDGF-mediated cell migration through ECM-integrin signaling in rats.", "gemini": "Mặc dù có nhiều bằng chứng về hoạt tính sinh học sau khi bức xạ hồng ngoại xa (FIR), nhưng tính nhạy cảm của tín hiệu tế bào đối với cân bằng nội môi do bức xạ FIR gây ra vẫn chưa được hiểu rõ. Để quan sát các tác động của bức xạ FIR, vải phủ vật liệu bức xạ FIR đã được đặt trên chuột thí nghiệm hoặc áp dụng cho tế bào L6 và phân tích mảng vi mô, phản ứng chuỗi polymerase thời gian thực định lượng và xét nghiệm lành vết thương đã được thực hiện. Phân tích mảng vi mô cho thấy biểu hiện RNA thông tin của cơ chuột được kích thích bởi bức xạ FIR theo cách phụ thuộc vào liều lượng ở lượng vật liệu phủ 10% và 30%. Trong nhóm 30%, 1.473 gen biểu hiện khác biệt đã được xác định (thay đổi lần [FC]> 1,5) và 218 gen được điều chỉnh đáng kể (FC> 1,5 và p <0,05). Phân tích mảng vi mô cho thấy tương tác ma trận ngoại bào (ECM) - thụ thể, bám dính cục bộ và các con đường liên quan đến di chuyển tế bào được kích thích đáng kể ở cơ chuột. Các gen liên quan đến di chuyển tế bào do ECM và yếu tố tăng trưởng có nguồn gốc từ tiểu cầu (PDGF) trung gian đã tăng lên. Và, kết quả cho thấy biểu hiện gen tương đối của actin beta tăng lên. Bức xạ FIR cũng kích thích các gen tiểu đơn vị actin và liên quan đến actin. Chúng tôi quan sát thấy rằng quá trình lành vết thương chắc chắn được thúc đẩy bởi bức xạ FIR trong hơn 48 giờ ở các tế bào L6. Do đó, chúng tôi cho rằng bức xạ FIR có thể xâm nhập vào cơ thể và kích thích quá trình di chuyển tế bào do PDGF trung gian thông qua tín hiệu ECM-integrin ở chuột."} +{"idx": 1900007, "en": "Pruritus (itching) is classically defined as an unpleasant cutaneous sensation that leads to scratching behavior. Although the scientific criteria of classification for pruritic diseases are not clear, it can be divided as acute or chronic by duration of symptoms. In this study, we investigated whether skin injury caused by chemical (contact hypersensitivity, CHS) or physical (skin-scratching stimulation, SSS) stimuli causes initial pruritus and analyzed gene expression profiles systemically to determine how changes in skin gene expression in the affected area are related to itching. In both CHS and SSS, we ranked the Gene Ontology Biological Process terms that are generally associated with changes. The factors associated with upregulation were keratinization, inflammatory response and neutrophil chemotaxis. The Kyoto Encyclopedia of Genes and Genomes pathway shows the difference of immune system, cell growth and death, signaling molecules and interactions, and signal transduction pathways. Il1a , Il1b and Il22 were upregulated in the CHS, and Tnf, Tnfrsf1b, Il1b, Il1r1 and Il6 were upregulated in the SSS. Trpc1 channel genes were observed in representative itching-related candidate genes. By comparing and analyzing RNA-sequencing data obtained from the skin tissue of each animal model in these characteristic stages, it is possible to find useful diagnostic markers for the treatment of itching, to diagnose itching causes and to apply customized treatment.", "gemini": "Ngứa được định nghĩa cổ điển là cảm giác khó chịu trên da dẫn đến hành vi gãi. Mặc dù các tiêu chí khoa học để phân loại bệnh ngứa không rõ ràng, nhưng có thể chia thành cấp tính hoặc mãn tính theo thời gian của các triệu chứng. Trong nghiên cứu này, chúng tôi đã điều tra xem tổn thương da do hóa chất (nhạy cảm tiếp xúc, CHS) hoặc kích thích vật lý (kích thích gãi da, SSS) có gây ngứa ban đầu hay không và phân tích hồ sơ biểu hiện gen một cách có hệ thống để xác định cách thay đổi biểu hiện gen da ở vùng bị ảnh hưởng liên quan đến ngứa như thế nào. Trong cả CHS và SSS, chúng tôi xếp hạng các thuật ngữ Quá trình sinh học của Gene Ontology thường liên quan đến các thay đổi. Các yếu tố liên quan đến điều hòa tăng lên là sừng hóa, đáp ứng viêm và hướng động bạch cầu trung tính. Con đường Bách khoa toàn thư về gen và bộ gen của Kyoto cho thấy sự khác biệt về hệ thống miễn dịch, sự phát triển và chết của tế bào, các phân tử tín hiệu và tương tác và các con đường truyền tín hiệu. Il1a, Il1b và Il22 được điều hòa tăng lên trong CHS, và Tnf, Tnfrsf1b, Il1b, Il1r1 và Il6 được điều hòa tăng lên trong SSS. Các gen kênh Trpc1 được quan sát thấy trong các gen ứng viên liên quan đến ngứa tiêu biểu. Bằng cách so sánh và phân tích dữ liệu giải trình tự RNA thu được từ mô da của từng mô hình động vật trong các giai đoạn đặc trưng này, có thể tìm ra các dấu hiệu chẩn đoán hữu ích cho việc điều trị ngứa, chẩn đoán nguyên nhân ngứa và áp dụng phương pháp điều trị tùy chỉnh."} +{"idx": 1900008, "en": "The derbid genus Vekunta Distant, 1906 is reviewed. Two new species, V.bambusana sp. n. and V.pentaprocessusa sp. n., are described and illustrated from the southwest of China to give the genus twenty-nine species in China. A checklist and a key to species of the genus from China are also provided.", "gemini": "Đã xem xét lại chi Derbid Vekunta Distant, 1906. Hai loài mới, V.bambusana sp. n. và V.pentaprocessusa sp. n., được mô tả và minh họa từ tây nam Trung Quốc để đưa chi này thành hai mươi chín loài ở Trung Quốc. Một danh sách kiểm tra và một chìa khóa cho các loài của chi từ Trung Quốc cũng được cung cấp."} +{"idx": 1900009, "en": "Background A parasitoid wasp, Trissolcus solocis Johnson, was recorded parasitising eggs of the invasive stink bug Halyomorpha halys (Stål), in the United States. This is the first record of this species parasitising eggs of H. halys. New information First record of Trissolcus solocis parasitising Halyomorpha halys eggs in the United States and first record of T. solocis in Alabama.", "gemini": "Bối cảnh Một loài ong ký sinh Trissolcus solocis Johnson, được ghi nhận là ký sinh trứng của loài rệp gây hại xâm lấn Halyomorpha halys (Stål), tại Hoa Kỳ. Đây là ghi nhận đầu tiên về loài này ký sinh trứng của H. halys. Thông tin mới Ghi nhận đầu tiên về Trissolcus solocis ký sinh trứng Halyomorpha halys tại Hoa Kỳ và ghi nhận đầu tiên về T. solocis tại Alabama."} +{"idx": 1900010, "en": "Background Cham Islands (Cu Lao Cham) is a group of 8 small islands in the Quang Nam province, Central Vietnam. There is only one study that mentioned the diversity of marine molluscs in this area. However, the data on species composition have not been digitised and not stored or shared for other purposes. Our paper aims to share the checklist of marine mollusc species (Bivalvia and Gastropoda) species that we collected from the littoral zone of Cham Islands in May 2017. This is the first digitised and online data of marine molluscs in Vietnam. It is very important for researchers in various fields such as the structure and function of ecology and biodiversity monitoring. New information This study provides a checklist of the marine bivalves and snails in the Cham Islands of Vietnam. Moreover, this first widely shared data of biodiversity in Vietnam can trigger biodiversity data papers in this data-poor country.The data of this study will be important inputs for better understanding biodiversity on the Cham Islands and Vietnam as well as for forming the basis for monitoring, exploitation and conservation of biodiversity in this area.In total, 145 taxa were recorded, 46 bivalve taxa and 99 snail taxa, from which 128 were identified to the species level and 17 were identified to the genus level. There are 116 new species records for the Cham Islands.The specimens are currently deposited in the Institute of Ecology and Biological Resources (IEBR), Vietnam Academy of Science and Technology (VAST).", "gemini": "Bối cảnh Quần đảo Chàm (Cù Lao Chàm) là một nhóm gồm 8 đảo nhỏ thuộc tỉnh Quảng Nam, miền Trung Việt Nam. Chỉ có một nghiên cứu đề cập đến sự đa dạng của động vật thân mềm biển trong khu vực này. Tuy nhiên, dữ liệu về thành phần loài chưa được số hóa và không được lưu trữ hoặc chia sẻ cho các mục đích khác. Bài báo của chúng tôi nhằm chia sẻ danh sách kiểm tra các loài thân mềm biển (Bivalvia và Gastropoda) mà chúng tôi thu thập được từ vùng ven bờ của Quần đảo Chàm vào tháng 5 năm 2017. Đây là dữ liệu đầu tiên được số hóa và trực tuyến về động vật thân mềm biển ở Việt Nam. Nó rất quan trọng đối với các nhà nghiên cứu trong các lĩnh vực khác nhau như cấu trúc và chức năng của sinh thái và giám sát đa dạng sinh học. Thông tin mới Nghiên cứu này cung cấp một danh sách kiểm tra về các loài nhuyễn thể và ốc biển ở Quần đảo Chàm của Việt Nam. Hơn nữa, dữ liệu về đa dạng sinh học được chia sẻ rộng rãi đầu tiên này tại Việt Nam có thể kích hoạt các bài báo về dữ liệu đa dạng sinh học ở quốc gia còn nghèo dữ liệu này. Dữ liệu của nghiên cứu này sẽ là những đầu vào quan trọng để hiểu rõ hơn về đa dạng sinh học trên Quần đảo Chàm và Việt Nam cũng như để hình thành cơ sở cho việc giám sát, khai thác và bảo tồn đa dạng sinh học trong khu vực này. Tổng cộng, 145 loài đã được ghi nhận, 46 loài hai mảnh vỏ và 99 loài ốc, trong đó 128 loài được xác định đến cấp loài và 17 loài được xác định đến cấp chi. Có 116 loài mới được ghi nhận ở Quần đảo Chàm. Các mẫu vật hiện đang được lưu giữ tại Viện Sinh thái và Tài nguyên sinh vật (IEBR), Viện Hàn lâm Khoa học và Công nghệ Việt Nam (VAST)."} +{"idx": 1900011, "en": "Background This paper provides a quantitative and general description of the Lyell Collection kept in the Department of Earth Sciences at the Natural History Museum of London. This collection started to be built by the eminent British geologist Sir Charles Lyell (1797-1875) in 1846 when the first specimen reached the Museum. The last one entered in 1980 donated by one of Lyell's heirs. There are more than 1700 specimens, mainly hand specimens with 93% of the fauna and flora from the Cenozoic of the Macaronesian archipelagos of the Canaries and Madeira. Those specimens that belong to the Lyell Collection with certainty have been databased and imaged. Currently they are being geo-referred automatically with the rest of the site geo-references at the NHM. This collection could be increased by a couple of dozen more specimens with those specimens located in the same drawers, but they do not have collector details. The work of data collection of these specimens was implemented over a year from 2016 to 2017, including annelids; brachiopods; bryozoans; echinoderms; scyphozoans; bivalves; gastropods; scaphopods; trilobites; plants; reptiles; fishes; and mammals. New information Access to the specimen-level data is available through the NHM data portal with the images associated. This is the first time that a description of the Fossil Lyell Collection dataset is available in the literature.", "gemini": "Bối cảnh Bài báo này cung cấp mô tả định lượng và tổng quát về Bộ sưu tập Lyell được lưu giữ tại Khoa Khoa học Trái đất thuộc Bảo tàng Lịch sử Tự nhiên London. Bộ sưu tập này bắt đầu được xây dựng bởi nhà địa chất học người Anh lỗi lạc Sir Charles Lyell (1797-1875) vào năm 1846 khi mẫu vật đầu tiên được đưa đến Bảo tàng. Mẫu cuối cùng được đưa vào năm 1980 do một trong những người thừa kế của Lyell tặng. Có hơn 1700 mẫu vật, chủ yếu là mẫu vật cầm tay với 93% động vật và thực vật từ kỷ Cenozoic của quần đảo Macaronesian ở Quần đảo Canary và Madeira. Những mẫu vật chắc chắn thuộc về Bộ sưu tập Lyell đã được lập cơ sở dữ liệu và chụp ảnh. Hiện chúng đang được tham chiếu địa lý tự động với các tham chiếu địa lý còn lại của địa điểm tại NHM. Bộ sưu tập này có thể được tăng thêm một vài chục mẫu vật nữa với những mẫu vật nằm trong cùng ngăn kéo, nhưng chúng không có thông tin chi tiết về người thu thập. Công việc thu thập dữ liệu của các mẫu vật này được thực hiện trong hơn một năm từ năm 2016 đến năm 2017, bao gồm giun đốt; động vật tay cuộn; động vật hình rêu; động vật da gai; động vật dạng đĩa; động vật hai mảnh vỏ; động vật chân bụng; động vật chân vảy; động vật ba thùy; thực vật; bò sát; cá; và động vật có vú. Thông tin mới Có thể truy cập dữ liệu cấp mẫu vật thông qua cổng dữ liệu NHM với các hình ảnh liên quan. Đây là lần đầu tiên mô tả về bộ dữ liệu Bộ sưu tập Lyell hóa thạch có trong các tài liệu."} +{"idx": 1900012, "en": "Twelve Ctenomyces (Arthrodermataceae, Onygenales) strains were obtained and identified during a survey of keratinophilic fungi in soils from China. We used molecular identification combined with morphological evidence to delimit species, circumscribing five species in the genus. Three new species are herein described: C.albus sp. nov., C.obovatus sp. nov. and C.peltricolor sp. nov. We also described, illustrated and compared the novel species with related species in the morphology.", "gemini": "Mười hai chủng Ctenomyces (Arthrodermataceae, Onygenales) đã được thu thập và xác định trong quá trình khảo sát nấm ưa keratin trong đất từ Trung Quốc. Chúng tôi đã sử dụng phương pháp nhận dạng phân tử kết hợp với bằng chứng về hình thái để phân định loài, giới hạn năm loài trong chi. Ba loài mới được mô tả ở đây: C.albus sp. nov., C.obovatus sp. nov. và C.peltricolor sp. nov. Chúng tôi cũng mô tả, minh họa và so sánh các loài mới với các loài liên quan về mặt hình thái."} +{"idx": 1900013, "en": "Here, we test the current generic delimitation of Rostania (Collemataceae, Peltigerales, Ascomycota) utilizing molecular phylogeny and morphological investigations. Using DNA sequence data from the mitochondrial SSU rDNA and two nuclear protein-coding genes (MCM7 and β-tubulin) and utilizing parsimony, maximum likelihood and Bayesian phylogenetic methods, Rostania is shown to be non-monophyletic in the current sense. A new generic delimitation of Rostania is thus proposed, in which the genus is monophyletic, and three species (Rostaniacoccophylla, R.paramensis, R.quadrifida) are excluded and transferred to other genera. Rostaniaoccultata is further non-monophyletic, and a more detailed investigation of species delimitations in Rostania s. str. is needed. The new combinations Leptogiumparamense and Scytiniumquadrifidum are proposed.", "gemini": "Ở đây, chúng tôi kiểm tra ranh giới chung hiện tại của Rostania (Collemataceae, Peltigerales, Ascomycota) bằng cách sử dụng phương pháp phát sinh loài phân tử và các cuộc điều tra về hình thái. Sử dụng dữ liệu trình tự DNA từ SSU rDNA của ty thể và hai gen mã hóa protein hạt nhân (MCM7 và β-tubulin) và sử dụng các phương pháp phát sinh loài tối ưu, khả năng tối đa và Bayes, Rostania được chứng minh là không đơn ngành theo nghĩa hiện tại. Do đó, một ranh giới chung mới của Rostania được đề xuất, trong đó chi này là đơn ngành và ba loài (Rostaniacoccophylla, R.paramensis, R.quadrifida) bị loại trừ và chuyển sang các chi khác. Rostaniaoccultata không phải là đơn ngành và cần có một cuộc điều tra chi tiết hơn về ranh giới loài trong Rostania s. str. Các tổ hợp mới Leptogiumparamense và Scytiniumquadrifidum được đề xuất."} +{"idx": 1900014, "en": "Knowledge on the diversity of hypogeous sequestrate ascomycetes is still limited in the Balkan Peninsula. A new species of truffle, Tuberpulchrosporum, is described from Greece and Bulgaria. Specimens were collected from habitats dominated by various oak species (i.e. Quercusilex, Q.coccifera, Q.robur) and other angiosperms. They are morphologically characterised by subglobose, ovoid to irregularly lobed, yellowish-brown to dark brown ascomata, usually with a shallow basal cavity and surface with fissures and small, dense, almost flat, trihedral to polyhedral warts. Ascospores are ellipsoid to subfusiform, uniquely ornamented, crested to incompletely reticulate and are produced in (1-)2-8-spored asci. Hair-like, hyaline to light yellow hyphae protrude from the peridium surface. According to the outcome of ITS rDNA sequence analysis, this species forms a distinct well-supported group in the Aestivum clade, with T.panniferum being the closest phylogenetic taxon.", "gemini": "Kiến thức về sự đa dạng của nấm ascomycetes dưới đất vẫn còn hạn chế ở Bán đảo Balkan. Một loài nấm cục mới, Tuberpulchrosporum, được mô tả từ Hy Lạp và Bulgaria. Các mẫu vật được thu thập từ các môi trường sống có nhiều loài sồi khác nhau (tức là Quercusilex, Q.coccifera, Q.robur) và các loài thực vật hạt kín khác. Chúng được đặc trưng về mặt hình thái bởi các ascomata hình cầu, hình trứng đến hình thùy không đều, màu nâu vàng đến nâu sẫm, thường có khoang đáy nông và bề mặt có vết nứt và các nốt sần nhỏ, dày đặc, gần như phẳng, hình tam diện đến hình đa diện. Bào tử hình elip đến hình gần hình thoi, có hoa văn độc đáo, có mào đến dạng lưới không hoàn chỉnh và được tạo ra trong các túi bào tử (1-)2-8. Các sợi nấm trong suốt đến màu vàng nhạt, giống như sợi tóc nhô ra từ bề mặt vỏ quả. Theo kết quả phân tích trình tự ITS rDNA, loài này tạo thành một nhóm riêng biệt được hỗ trợ tốt trong nhánh Aestivum, với T.panniferum là đơn vị phân loại phát sinh loài gần nhất."} +{"idx": 1900015, "en": "The ethyl acetate extract of the culture filtrate of Phlebia tremellosa promoted elongation of the lateral roots of lettuce seedlings at 250 µg/mL. We purified two compounds that promote root elongation by using activity-guided chromatographic fractionation. On the basis of spectroscopic analyses, these compounds were identified to be isolactarane sesquiterpenes derived from the dehydrogenation of merulactone, which was previously isolated from the same species. We named the purified compounds phlelactones A and B. Phlelactones A and B promoted primary root elongation at 100-300 and 10-30 µg/mL and the elongation and formation of lateral roots at 300-1000 and 30-100 µg/mL, respectively.", "gemini": "Chiết xuất ethyl acetate từ dịch lọc nuôi cấy của Phlebia tremellosa thúc đẩy sự kéo dài của rễ bên của cây con rau diếp ở nồng độ 250 µg/mL. Chúng tôi đã tinh chế hai hợp chất thúc đẩy sự kéo dài của rễ bằng cách sử dụng phương pháp phân đoạn sắc ký hướng dẫn hoạt động. Trên cơ sở phân tích quang phổ, những hợp chất này được xác định là sesquiterpen isolactarane có nguồn gốc từ quá trình dehydrogen hóa của merulactone, trước đây được phân lập từ cùng một loài. Chúng tôi đặt tên cho các hợp chất tinh chế là phlelactone A và B. Phlelactone A và B thúc đẩy sự kéo dài của rễ chính ở nồng độ 100-300 và 10-30 µg/mL và sự kéo dài và hình thành của rễ bên ở nồng độ 300-1000 và 30-100 µg/mL."} +{"idx": 1900016, "en": "Phytophagous insects utilize visual, olfactory and gustatory cues to find food. The brown marmorated stink bug, Halyomorpha halys (Stål), quickly approaches fresh peanut seeds newly introduced into the rearing cage in the laboratory but shows less interest in stale peanut seed previously infested by conspecifics. This observation suggests that H. halys can perceive the quality of food by detecting the volatile(s) from fresh peanut seeds. A bioassay revealed that H. halys adults could more quickly find fresh peanut seeds than three-day-infested peanut seeds, which is consistent with laboratory observations. Hexanal was found to be the major volatile component of fresh peanut seeds but not of previously infested ones. In the two-choice assays, the adult bugs that did respond were significantly attracted to fresh peanut volatiles and hexanal. Hexanal also induced proboscis-protruding behavior in adult H. halys, which suggested that this compound is a key stimulant of foraging behavior of laboratory-reared H. halys adults.", "gemini": "Côn trùng ăn thực vật sử dụng các tín hiệu thị giác, khứu giác và vị giác để tìm thức ăn. Bọ xít hôi cẩm thạch nâu, Halyomorpha halys (Stål), nhanh chóng tiếp cận những hạt đậu phộng tươi mới được đưa vào lồng nuôi trong phòng thí nghiệm nhưng ít quan tâm đến hạt đậu phộng ôi trước đó đã bị những con cùng loài xâm nhập. Quan sát này cho thấy H. halys có thể nhận biết chất lượng của thức ăn bằng cách phát hiện ra chất dễ bay hơi từ hạt đậu phộng tươi. Một xét nghiệm sinh học cho thấy những con H. halys trưởng thành có thể tìm thấy hạt đậu phộng tươi nhanh hơn hạt đậu phộng bị nhiễm trong ba ngày, điều này phù hợp với các quan sát trong phòng thí nghiệm. Hexanal được phát hiện là thành phần dễ bay hơi chính của hạt đậu phộng tươi nhưng không phải của hạt đậu phộng đã bị nhiễm trước đó. Trong các xét nghiệm lựa chọn hai, những con bọ trưởng thành phản ứng bị thu hút đáng kể bởi chất dễ bay hơi từ đậu phộng tươi và hexanal. Hexanal cũng gây ra hành vi thè vòi ở H. halys trưởng thành, điều này cho thấy hợp chất này là chất kích thích chính cho hành vi kiếm ăn của H. halys trưởng thành được nuôi trong phòng thí nghiệm."} +{"idx": 1900017, "en": "Ipfencarbazone exhibits excellent herbicidal activity against Echinochloa spp. and is safe for rice. The effects of ipfencarbazone on very long chain fatty acid (VLCFA) elongation in rice and late watergrass and its inhibitory mechanism were investigated in this study. Although ipfencarbazone inhibited VLCFA elongation in the microsomes prepared from late watergrass and rice at low concentrations, the inhibitory effect was higher in late watergrass than in rice. These results suggested that the primary site of action of ipfencarbazone is VLCFA elongase (VLCFAE) and ipfencarbazone has a differential affinity between the VLCFAEs of the plants. The inhibitory activity of ipfencarbazone became higher in proportion to pre-incubation period with the VLCFAE. The degree of inhibition did not decrease by dilution of the VLCFAE-ipfencarbazone complex. These results suggested that ipfencarbazone binds to the VLCFAE irreversibly.", "gemini": "Ipfencarbazone thể hiện hoạt tính diệt cỏ tuyệt vời đối với Echinochloa spp. và an toàn cho lúa. Các tác động của ipfencarbazone đối với quá trình kéo dài axit béo chuỗi rất dài (VLCFA) ở lúa và cỏ lồng vực muộn và cơ chế ức chế của nó đã được nghiên cứu trong nghiên cứu này. Mặc dù ipfencarbazone ức chế quá trình kéo dài VLCFA trong các vi thể chế biến từ cỏ lồng vực muộn và lúa ở nồng độ thấp, nhưng hiệu quả ức chế ở cỏ lồng vực muộn cao hơn ở lúa. Những kết quả này cho thấy vị trí tác động chính của ipfencarbazone là elongase VLCFA (VLCFAE) và ipfencarbazone có ái lực khác nhau giữa VLCFAE của các loài thực vật. Hoạt tính ức chế của ipfencarbazone tăng cao theo tỷ lệ với thời gian tiền ủ với VLCFAE. Mức độ ức chế không giảm khi pha loãng phức hợp VLCFAE-ipfencarbazone. Những kết quả này cho thấy ipfencarbazone liên kết không thể đảo ngược với VLCFAE."} +{"idx": 1900018, "en": "The relationship between the nucleotide sequences of CYP51, its expression level and its sensitivity to ipconazole of Fusarium fujikuroi isolates were investigated. Single nucleotide polymorphisms (SNPs) were observed in the CYP51 of isolates with different sensitivities to ipconazole, but no amino acid substitution was detected in the putative amino acid sequence of the CYP51 protein. On the other hand, the expression of CYP51 was enhanced by the presence of ipconazole, and it tended to be higher in isolates with lower sensitivities and no gibberellin productivity. In the presumed promoter region, the upstream nucleotide sequence of CYP51, several common SNPs and insertions of nucleotides were detected in the lower sensitivity isolates. These results suggest that F. fujikuroi isolates consist of 2 different groups in sensitivity and gibberellin productivity, and no amino acid substitution in CYP51 protein may contribute to the stably high efficacy of ipconazole against rice Bakanae disease for more than 25 years.", "gemini": "Mối quan hệ giữa trình tự nucleotide của CYP51, mức độ biểu hiện của nó và độ nhạy của nó đối với ipconazole của các phân lập Fusarium fujikuroi đã được nghiên cứu. Đa hình nucleotide đơn (SNP) được quan sát thấy ở CYP51 của các phân lập có độ nhạy khác nhau đối với ipconazole, nhưng không phát hiện thấy sự thay thế axit amin nào trong trình tự axit amin giả định của protein CYP51. Mặt khác, sự biểu hiện của CYP51 được tăng cường bởi sự hiện diện của ipconazole và nó có xu hướng cao hơn ở các phân lập có độ nhạy thấp hơn và không có năng suất gibberellin. Trong vùng khởi động được cho là, trình tự nucleotide thượng nguồn của CYP51, một số SNP phổ biến và sự chèn nucleotide đã được phát hiện ở các phân lập có độ nhạy thấp hơn. Những kết quả này cho thấy rằng các phân lập F. fujikuroi bao gồm 2 nhóm khác nhau về độ nhạy và năng suất gibberellin, và không có sự thay thế axit amin nào trong protein CYP51 có thể góp phần vào hiệu quả cao ổn định của ipconazole đối với bệnh Bakanae trên cây lúa trong hơn 25 năm."} +{"idx": 1900019, "en": "The purpose of this study is to isolate the beneficial microorganisms whose growth is promoted in the presence of charcoal materials. We successfully isolated strain IA, whose growth is promoted on an agar plate with charcoal materials, and identified it as a novel strain of the Bacillus sp. The growth of strain IA in the liquid medium was promoted by the addition of both activated charcoal (AC) and rice husk biochar (RHB). Moreover, the sporulation of strain IA in the RHB medium and the antifungal activity of the culture supernatant of the RHB medium were much higher than those with AC. HPLC and MS analyses revealed that strain IA produced an antifungal lipopeptide iturin A, and the yield of iturin A in the RHB medium was 8 times higher than that in the medium without RHB. This is the first paper to describe the positive effect of RHB on microbial metabolisms.", "gemini": "Mục đích của nghiên cứu này là phân lập các vi sinh vật có lợi có sự phát triển được thúc đẩy khi có sự hiện diện của vật liệu than. Chúng tôi đã phân lập thành công chủng IA, có sự phát triển được thúc đẩy trên đĩa thạch có vật liệu than và xác định nó là một chủng mới của Bacillus sp. Sự phát triển của chủng IA trong môi trường lỏng được thúc đẩy bởi sự bổ sung của cả than hoạt tính (AC) và than sinh học vỏ trấu (RHB). Hơn nữa, quá trình bào tử hóa của chủng IA trong môi trường RHB và hoạt tính kháng nấm của dịch nuôi cấy của môi trường RHB cao hơn nhiều so với môi trường có AC. Các phân tích HPLC và MS cho thấy chủng IA sản xuất ra một lipopeptit kháng nấm iturin A và năng suất iturin A trong môi trường RHB cao gấp 8 lần so với môi trường không có RHB. Đây là bài báo đầu tiên mô tả tác động tích cực của RHB đối với quá trình trao đổi chất của vi sinh vật."} +{"idx": 1900020, "en": "Ultrasound, which refers to frequencies above the audible limit of human hearing, is a candidate for inducing resistance to pathogens in plants. We revealed that aerial ultrasound of 40.5 kHz could induce disease resistance in tomatoes and rice when the plants were irradiated with ultrasound of ca. 100 dB for 2 weeks during nursery season and reduced the incidence of Fusarium wilt and blast diseases, respectively, when plants were inoculated with pathogen 0 or 1 week after terminating irradiation. Disease control efficacy was also observed with ultrasound at frequencies of 19.8 and 28.9 kHz. However, cabbage yellows and powdery mildew on lettuce were not suppressed by ultrasound irradiation. No significant positive or negative effect on growth was observed in tomato and rice plants. RT-qPCR showed that the expression of PR1a involved in the salicylic acid (SA) signaling pathway was upregulated in the ultrasound-irradiated tomato.", "gemini": "Siêu âm, đề cập đến các tần số trên giới hạn nghe được của con người, là một ứng cử viên để gây ra khả năng kháng bệnh ở thực vật. Chúng tôi phát hiện ra rằng siêu âm trên không với tần số 40,5 kHz có thể gây ra khả năng kháng bệnh ở cà chua và lúa khi cây được chiếu siêu âm khoảng 100 dB trong 2 tuần trong thời kỳ ươm và làm giảm tỷ lệ bệnh héo Fusarium và bệnh đạo ôn tương ứng khi cây được tiêm mầm bệnh 0 hoặc 1 tuần sau khi kết thúc chiếu xạ. Hiệu quả kiểm soát bệnh cũng được quan sát thấy với siêu âm ở tần số 19,8 và 28,9 kHz. Tuy nhiên, bệnh vàng lá trên bắp cải và bệnh phấn trắng trên rau diếp không bị ức chế bởi chiếu siêu âm. Không quan sát thấy tác động tích cực hoặc tiêu cực đáng kể nào đối với sự phát triển của cây cà chua và lúa. RT-qPCR cho thấy rằng sự biểu hiện của PR1a liên quan đến con đường truyền tín hiệu axit salicylic (SA) được điều hòa tăng lên ở cây cà chua được chiếu siêu âm."} +{"idx": 1900021, "en": "Three-year comprehensive experiments were conducted to compare the dissipation patterns of a total of 16 pesticides, including 3 metabolites, as granular formulations applied in lysimeters and paddy fields with two soil types. Analytical concentrations of the target pesticides in paddy water were analyzed using a granular kinetic model consisting of the following parameters: release rate, decrease rate, and dissolved concentration. Results of parameter grouping analyses of the kinetic models showed that 56% of data reproducibility (entire grouping) was obtained between replicates for the lysimeters. In comparisons between the lysimeters and paddy fields, 48% of decrease rates and 34% of release rates were grouped, although significant differences were observed with a nearly 90% difference for dissolved concentrations. These differences might be attributed to the hydrological components such as water management and meteorological covariates in paddy fields, the daily percolation in lysimeters and the adsorption-desorption kinetics between paddy water and soil.", "gemini": "Các thí nghiệm toàn diện kéo dài ba năm đã được tiến hành để so sánh các kiểu tiêu tán của tổng cộng 16 loại thuốc trừ sâu, bao gồm 3 chất chuyển hóa, dưới dạng chế phẩm dạng hạt được sử dụng trong các bình đo lysimeter và ruộng lúa có hai loại đất. Nồng độ phân tích của các loại thuốc trừ sâu mục tiêu trong nước ruộng đã được phân tích bằng cách sử dụng mô hình động học hạt bao gồm các thông số sau: tỷ lệ giải phóng, tỷ lệ giảm và nồng độ hòa tan. Kết quả phân tích nhóm tham số của các mô hình động học cho thấy 56% khả năng tái tạo dữ liệu (toàn bộ nhóm) thu được giữa các bản sao đối với các bình đo lysimeter. Khi so sánh giữa các bình đo lysimeter và ruộng lúa, có 48% tỷ lệ giảm và 34% tỷ lệ giải phóng được nhóm lại, mặc dù có sự khác biệt đáng kể với sự khác biệt gần 90% đối với nồng độ hòa tan. Những khác biệt này có thể là do các thành phần thủy văn như quản lý nước và các biến số khí tượng trong ruộng lúa, sự thẩm thấu hàng ngày trong các bình đo lysimeter và động học hấp phụ-giải hấp phụ giữa nước ruộng và đất."} +{"idx": 1900022, "en": "Comparative experiments investigating the dissipation of four nursery-box-applied pesticides and three foliar-applied pesticides were conducted using lysimeters and in actual paddy fields. In the lysimeter experiments, there were test plots for submerged application for both application types. Analytical concentrations of the pesticides in paddy water were evaluated using appropriate kinetic models. The detection levels of pesticides in the paddy water for the nursery-box and foliar applications were 10-77% and 42-79% of the submerged application, respectively. The times required for 50% dissipation (DT 50s) in case of the nursery-box and foliar applications were 0.8-10.4 days and 0.5-2.7 days, respectively. Although overall dissipations were affected by the physicochemical properties of the pesticide and the experimental design in the test plots, the initial detection levels in the lysimeters, governed by the runoff at transplanting and the deposition at spraying, were comparable with those in the actual paddy fields.", "gemini": "Các thí nghiệm so sánh để nghiên cứu sự tiêu tán của bốn loại thuốc trừ sâu được sử dụng trong hộp ươm và ba loại thuốc trừ sâu được sử dụng trên lá đã được tiến hành bằng cách sử dụng các bình đo lysimeter và trên các ruộng lúa thực tế. Trong các thí nghiệm lysimeter, có các ô thử để ứng dụng chìm đối với cả hai loại ứng dụng. Nồng độ phân tích của thuốc trừ sâu trong nước ruộng đã được đánh giá bằng các mô hình động học thích hợp. Mức độ phát hiện thuốc trừ sâu trong nước ruộng đối với các ứng dụng hộp ươm và ứng dụng trên lá lần lượt là 10-77% và 42-79% so với ứng dụng chìm. Thời gian cần thiết để tiêu tán 50% (DT 50) trong trường hợp ứng dụng hộp ươm và ứng dụng trên lá lần lượt là 0,8-10,4 ngày và 0,5-2,7 ngày. Mặc dù sự tiêu tán tổng thể bị ảnh hưởng bởi các tính chất lý hóa của thuốc trừ sâu và thiết kế thử nghiệm trong các ô thử, nhưng mức độ phát hiện ban đầu trong các bình đo lysimeter, được chi phối bởi dòng chảy khi cấy và sự lắng đọng khi phun, tương đương với mức độ trong các ruộng lúa thực tế."} +{"idx": 1900023, "en": "γ-BHC and dieldrin are legacy insecticides that were extensively used after the second World War. When they were banned, their modes of action and metabolism were not known. This article aims at providing a picture of the metabolism of γ-BHC and the modes of action of γ -BHC and dieldrin. γ-BHC is metabolized via two independent metabolic pathways. One is a glutathione conjugation pathway resulting in the formation of dichlorophenyl mercapturic acid and the other is an oxidative metabolism catalyzed by microsomes to mainly 2,4,6-trichlorophenol (TCP) and (36/45)-1,2,3,4,5,6-hexachlorocyclohex-1-ene (HCCHE). Other metabolites of this pathway are 2,4,5-TCP, 2,3,4,6-tetrachlorophenol (TeCP), (36/45)- and (346/5)-1,3,4,5,6-pentachlorocyclohex-1-enes (PCCHE). Nowadays, γ-BHC and dieldrin are very important reagents which are used to study the GABA receptor in insects and mammals. They were found to be noncompetitive GABA antagonists blocking the chloride ion selective pores in the GABA-gated chloride channels and leading to inhibition of chloride ion conductance. [3H]EBOB binding data showed that γ-BHC, its analogs, dieldrin, and other cyclodiene insecticides interact with the same site on GABA receptor as picrotoxinin. Only γ-BHC, among other BHC isomers, exhibits this binding characteristic. Milbemycin, currently widely used as an insecticide, acaricide and nematicide, has been found to open the GABA-gated chloride channel.", "gemini": "γ-BHC và dieldrin là thuốc trừ sâu thế hệ cũ được sử dụng rộng rãi sau Thế chiến thứ hai. Khi chúng bị cấm, người ta vẫn chưa biết về cơ chế hoạt động và quá trình chuyển hóa của chúng. Bài viết này nhằm mục đích cung cấp một bức tranh về quá trình chuyển hóa của γ-BHC và các chế độ hoạt động của γ -BHC và dieldrin. γ-BHC được chuyển hóa thông qua hai con đường chuyển hóa độc lập. Một là con đường liên hợp glutathione dẫn đến sự hình thành axit dichlorophenyl mercapturic và con đường còn lại là quá trình chuyển hóa oxy hóa được xúc tác bởi microsome thành chủ yếu là 2,4,6-trichlorophenol (TCP) và (36/45)-1,2,3,4,5,6-hexachlorocyclohex-1-ene (HCCHE). Các chất chuyển hóa khác của con đường này là 2,4,5-TCP, 2,3,4,6-tetrachlorophenol (TeCP), (36/45)- và (346/5)-1,3,4,5,6-pentachlorocyclohex-1-enes (PCCHE). Ngày nay, γ-BHC và dieldrin là những chất phản ứng rất quan trọng được sử dụng để nghiên cứu thụ thể GABA ở côn trùng và động vật có vú. Người ta thấy rằng chúng là chất đối kháng GABA không cạnh tranh, ngăn chặn các lỗ chọn lọc ion clorua trong các kênh clorua do GABA kiểm soát và dẫn đến ức chế dẫn truyền ion clorua. Dữ liệu liên kết [3H]EBOB cho thấy γ-BHC, các chất tương tự của nó, dieldrin và các thuốc trừ sâu cyclodiene khác tương tác với cùng một vị trí trên thụ thể GABA như picrotoxin. Chỉ có γ-BHC, trong số các đồng phân BHC khác, biểu hiện đặc tính liên kết này. Milbemycin, hiện được sử dụng rộng rãi như một loại thuốc trừ sâu, diệt bọ ve và tuyến trùng, đã được phát hiện là mở kênh clorua do GABA kiểm soát."} +{"idx": 1900024, "en": "Background Neopterin is a pyrazino-pyrimidine compound which is used as a marker of cell-mediated immunity in a variety of diseases. It is known that neopterin levels increase in diseases where interferon-gamma (IFN-g) stimulation is present, and also as a result of deficiencies in renal function, given that the primary means of elimination of neopterin is through the kidneys. In this study, we aimed to investigate the role of increased neopterin levels as a prognostic biomarker in patients with impaired renal function. Methods A total of 90 individuals including 63 patients with chronic kidney failure (CKF) and 27 healthy volunteers were included in the study. Serum neopterin concentrations were measured using the enzyme-linked immunosorbent assay. A Mann-Whitney U test and a Pearson Correlation Test were used in the statistical analysis, with a p value of <0.05 being considered statistically significant. Results The mean age was 52.21±0.16 years in the patient group and 56.55±0.32 years in the control group. In the CKF patients, serum neopterin levels increased to a significantly greater degree than in the control group (p<0.001), while no statistically significant correlation was identified between serum neopterin levels and age (p>0.05). Conclusions A significant increase was found in the serum neopterin levels in the CKF patients, due to both the triggering of the disease and the reduction of neopterin elimination.", "gemini": "Bối cảnh Neopterin là một hợp chất pyrazino-pyrimidine được sử dụng như một dấu hiệu của miễn dịch qua trung gian tế bào trong nhiều loại bệnh. Người ta biết rằng nồng độ neopterin tăng trong các bệnh có sự kích thích interferon-gamma (IFN-g) và cũng là kết quả của tình trạng suy giảm chức năng thận, vì thận là phương tiện chính để đào thải neopterin. Trong nghiên cứu này, chúng tôi nhằm mục đích điều tra vai trò của việc tăng nồng độ neopterin như một dấu ấn sinh học tiên lượng ở những bệnh nhân suy giảm chức năng thận. Phương pháp Tổng cộng có 90 cá nhân bao gồm 63 bệnh nhân suy thận mạn (CKF) và 27 tình nguyện viên khỏe mạnh được đưa vào nghiên cứu. Nồng độ neopterin trong huyết thanh được đo bằng phương pháp xét nghiệm miễn dịch liên kết với enzyme. Kiểm định Mann-Whitney U và Kiểm định Tương quan Pearson được sử dụng trong phân tích thống kê, với giá trị p <0,05 được coi là có ý nghĩa thống kê. Kết quả Độ tuổi trung bình là 52,21±0,16 tuổi ở nhóm bệnh nhân và 56,55±0,32 tuổi ở nhóm đối chứng. Ở những bệnh nhân CKF, nồng độ neopterin trong huyết thanh tăng lên đáng kể hơn so với nhóm đối chứng (p <0,001), trong khi không xác định được mối tương quan có ý nghĩa thống kê giữa nồng độ neopterin trong huyết thanh và tuổi (p> 0,05). Kết luận Nồng độ neopterin trong huyết thanh tăng đáng kể ở bệnh nhân CKF, do cả sự khởi phát của bệnh và sự giảm thải neopterin."} +{"idx": 1900025, "en": "Background Endocrine system plays a major role in both permissive and regulatory activities in order to adequately respond to physical stress of exercise. But level and direction of activation depend on many factors and are not easily interpreted. Methods We tested a group of male professional athletes (21 water polo players and 15 wrestlers), together with 20 sedentary controls matched by age. All participants took a continuous progressive exercise stress test on a treadmill until exhaustion and plateau of oxygen consumption (VO2). Blood samples for cortisol, sex hormone binding globulin (SHBG) and testosterone were drawn in four time points: baseline (B), start of the test (S), point of maximal strain (MAX) and in the 3rd minute of recovery period (R). Results Cortisol levels significantly increased in both groups, but the response between S and MAX was more pronounced in controls (p=0.036). The athletes had significantly higher levels of cortisol in all points in test, except during R (p=0.118), when their cortisol levels gradually started to decline. Significant increase in total testosterone was in great deal a consequence of increase in SHBG level (p<0.01 for both). Consequently, calculated free testosterone significantly decreased during test (p=0.008), and the drop was more pronounced in athletes. This was in concordance with significant correlation between SHBG and cortisol level demonstrated in athletes, but not in controls. Conclusions It seems that high intensity endurance exercise favors catabolic response, but the level of response highly depends on a previous level of training.", "gemini": "Bối cảnh Hệ thống nội tiết đóng vai trò chính trong cả hoạt động cho phép và hoạt động điều hòa để đáp ứng đầy đủ với căng thẳng thể chất khi tập thể dục. Nhưng mức độ và hướng kích hoạt phụ thuộc vào nhiều yếu tố và không dễ để diễn giải. Phương pháp Chúng tôi đã kiểm tra một nhóm vận động viên chuyên nghiệp nam (21 cầu thủ bóng nước và 15 đô vật), cùng với 20 đối tượng ít vận động được ghép theo độ tuổi. Tất cả những người tham gia đều thực hiện bài kiểm tra căng thẳng gắng sức tiến triển liên tục trên máy chạy bộ cho đến khi kiệt sức và mức tiêu thụ oxy (VO2) đạt đỉnh. Các mẫu máu để xét nghiệm cortisol, globulin gắn hormone sinh dục (SHBG) và testosterone được lấy tại bốn thời điểm: đường cơ sở (B), bắt đầu thử nghiệm (S), thời điểm căng thẳng tối đa (MAX) và phút thứ 3 của thời kỳ phục hồi (R). Kết quả Nồng độ cortisol tăng đáng kể ở cả hai nhóm, nhưng phản ứng giữa S và MAX rõ rệt hơn ở nhóm đối chứng (p = 0,036). Các vận động viên có nồng độ cortisol cao hơn đáng kể ở tất cả các thời điểm trong quá trình thử nghiệm, ngoại trừ trong thời gian R (p = 0,118), khi nồng độ cortisol của họ dần bắt đầu giảm. Sự gia tăng đáng kể về tổng lượng testosterone phần lớn là hậu quả của sự gia tăng mức SHBG (p <0,01 cho cả hai). Do đó, testosterone tự do được tính toán giảm đáng kể trong quá trình thử nghiệm (p = 0,008) và sự sụt giảm này rõ rệt hơn ở các vận động viên. Điều này phù hợp với mối tương quan đáng kể giữa SHBG và nồng độ cortisol được chứng minh ở các vận động viên, nhưng không phải ở nhóm đối chứng. Kết luận Có vẻ như bài tập sức bền cường độ cao có lợi cho phản ứng dị hóa, nhưng mức độ phản ứng phụ thuộc rất nhiều vào mức độ tập luyện trước đó."} +{"idx": 1900026, "en": "Background Mutations in BRCA1 gene have been implicated in ovarian cancers, and BRCA testing may be conducted in high-risk women. This study was designed to determine the frequency of three single nucleotide polymorphisms (SNPs) variants in BRCA1 gene and BRCA1 expression in Saudi females with ovarian cancer. Methods Expression levels of mRNA of BRCA1 gene were studied in 10 ovarian cancer and 10 normal ovarian tissues, by quantitative real time polymerase chain reaction (qPCR). The study also included 28 females who had suffered from ovarian cancer and had been successfully operated upon and 90 healthy females with no history of cancer. Blood was drawn in EDTA tubes and used for extraction of DNA. The genotyping was carried out using Taqman® SNP Genotyping kit by RT-PCR. The variants investigated included c.871 T>C (rs799917), c.1040 G>A (rs4986852), c.181 T>G (rs28897672) in BRCA1 gene. Results The c.181 T>G (rs28897672) showed significantly different genotype and allele frequencies between the patients and the control subjects (p value = 0.002 and 0.02, respectively). The genotype TG was significantly protective (OR = 0.36, p value = 0.024). The mRNA expression of BRCA1 gene was found to be low in the ovarian cancer tissues. Conclusions This study showed that c.181 T>G in BRCA1 genes is associated with the development of ovarian cancer in Saudis. More studies are needed to unveil other SNPs that may be associated with ovarian cancer and to understand the mechanism(s) involved in reducing the expression of BRCA1 gene in ovarian cancer tissues.", "gemini": "Bối cảnh Đột biến ở gen BRCA1 đã được chứng minh là liên quan đến ung thư buồng trứng và xét nghiệm BRCA có thể được thực hiện ở những phụ nữ có nguy cơ cao. Nghiên cứu này được thiết kế để xác định tần suất của ba biến thể đa hình một nucleotide (SNP) ở gen BRCA1 và biểu hiện BRCA1 ở phụ nữ Ả Rập mắc ung thư buồng trứng. Phương pháp Mức độ biểu hiện mRNA của gen BRCA1 đã được nghiên cứu ở 10 bệnh nhân ung thư buồng trứng và 10 mô buồng trứng bình thường, bằng phản ứng chuỗi polymerase thời gian thực định lượng (qPCR). Nghiên cứu cũng bao gồm 28 phụ nữ đã từng mắc ung thư buồng trứng và đã được phẫu thuật thành công và 90 phụ nữ khỏe mạnh không có tiền sử ung thư. Máu được lấy trong ống EDTA và sử dụng để chiết xuất DNA. Kiểu gen được thực hiện bằng bộ dụng cụ Đánh kiểu gen SNP Taqman® bằng RT-PCR. Các biến thể được nghiên cứu bao gồm c.871 T>C (rs799917), c.1040 G>A (rs4986852), c.181 T>G (rs28897672) ở gen BRCA1. Kết quả c.181 T>G (rs28897672) cho thấy tần số kiểu gen và tần số alen khác nhau đáng kể giữa những bệnh nhân và đối tượng đối chứng (giá trị p lần lượt là 0,002 và 0,02). Kiểu gen TG có khả năng bảo vệ đáng kể (OR = 0,36, giá trị p = 0,024). Biểu hiện mRNA của gen BRCA1 được phát hiện ở mức thấp trong các mô ung thư buồng trứng. Kết luận Nghiên cứu này cho thấy c.181 T>G ở gen BRCA1 có liên quan đến sự phát triển của ung thư buồng trứng ở người Ả Rập. Cần nhiều nghiên cứu hơn để phát hiện ra các SNP khác có thể liên quan đến ung thư buồng trứng và để hiểu cơ chế làm giảm biểu hiện của gen BRCA1 trong các mô ung thư buồng trứng."} +{"idx": 1900027, "en": "Background Laboratory thyroid function tests play a central role in the diagnosis of thyroid disorders. The aim of our cross-sectional study was to determine reference values for thyroid tests in a rigorously selected group of Montenegrin females, investigate the impact of possible age-related changes and the influence of the interassay bias between three frequently used immunoassays. Methods Female subjects were randomly selected, aged between 20 and 69 and 946 of them met the selection criteria. TSH, fT3, fT4, thyroid peroxidase and thyroglobulin antibodies were measured. Eighty samples were further analyzed on two other immunochemistry platforms. Results Median TSH progressively increased with age, there was no difference in fT3, while fT4 was significantly higher in the two oldest groups compared to the others. When using the age-related 97.5 percentile of TSH the percentage of reclassification was highest in the 20-29 years of age group (5.2%, p<0.05). In the oldest band, 7.7% had TSH values above cohort-specific and below the age-related upper reference limit. Bland-Altman bias plots revealed the highest interassay absolute mean difference between compared TSH assays of 24.5% and for fT4 assays of 13.8%. Conclusions The correlation coefficients between fT3 assays from different manufacturers were low. Serum TSH and fT4 concentrations increased with age and the implementation of age-specific TSH reference intervals would be of interest. The bias between the three commercial immunoassays indicated that the standardization of thyroid function tests is a task of great importance.", "gemini": "Lý lịch Các xét nghiệm chức năng tuyến giáp trong phòng xét nghiệm đóng vai trò trung tâm trong chẩn đoán các rối loạn tuyến giáp. Mục đích của nghiên cứu cắt ngang của chúng tôi là xác định các giá trị tham chiếu cho các xét nghiệm tuyến giáp trong một nhóm phụ nữ Montenegro được lựa chọn nghiêm ngặt, điều tra tác động của những thay đổi có thể liên quan đến tuổi tác và ảnh hưởng của sai số giữa ba xét nghiệm miễn dịch thường được sử dụng. Phương pháp Các đối tượng nữ được lựa chọn ngẫu nhiên, tuổi từ 20 đến 69 và 946 người trong số họ đáp ứng các tiêu chí lựa chọn. TSH, fT3, fT4, kháng thể peroxidase tuyến giáp và thyroglobulin được đo. Tám mươi mẫu đã được phân tích thêm trên hai nền tảng miễn dịch hóa học khác. Kết quả TSH trung bình tăng dần theo tuổi, không có sự khác biệt về fT3, trong khi fT4 cao hơn đáng kể ở hai nhóm lớn tuổi nhất so với các nhóm khác. Khi sử dụng phần trăm thứ 97,5 liên quan đến tuổi của TSH, tỷ lệ phân loại lại cao nhất ở nhóm tuổi 20-29 (5,2%, p <0,05). Trong nhóm lớn tuổi nhất, 7,7% có giá trị TSH cao hơn giới hạn tham chiếu trên cụ thể của nhóm và thấp hơn giới hạn tham chiếu trên liên quan đến tuổi. Biểu đồ sai số Bland-Altman cho thấy sự khác biệt trung bình tuyệt đối giữa các xét nghiệm TSH được so sánh cao nhất là 24,5% và đối với các xét nghiệm fT4 là 13,8%. Kết luận Hệ số tương quan giữa các xét nghiệm fT3 từ các nhà sản xuất khác nhau là thấp. Nồng độ TSH và fT4 trong huyết thanh tăng theo tuổi và việc áp dụng các khoảng tham chiếu TSH theo độ tuổi sẽ rất hữu ích. Sai số giữa ba xét nghiệm miễn dịch thương mại cho thấy việc chuẩn hóa các xét nghiệm chức năng tuyến giáp là một nhiệm vụ rất quan trọng."} +{"idx": 1900028, "en": "Background Hemoglobin A1c, (HbA1c) which is the major constituent of glycated hemoglobin, has been used in the follow-up of retrospective glycemia for years and in the diagnosis of diabetes mellitus nowadays. Since the analytical performance of HbA1c should be high likewise all laboratory tests, various quality control measures are used. Sigma metrics is one of these measures and it is the combination of bias, precision and total allowable error that ensures a general evaluation of analytical quality. The aim of our study was to evaluate the analytical performance of Bio-Rad's Variant Turbo II HbA1c analyzer according to sigma metrics. Methods Sigma levels were calculated using the data obtained from two levels of internal and 12 external quality control materials (Bio-Rad) of Variant II Turbo HbA1c analyzer according to σ = (TEa% - Bias%) / CV% formula. Results The mean sigma levels for low and high quality control materials were found to be 3.0 and 4.1, respectively. Conclusions The annual mean analytical performance of Variant II Turbo HbA1c analyzer was found to be acceptable according to sigma metrics. In order to be sure of the difference in HbA1c results indicating the success or failure in treatment but not arise from analytical variation, it is thought that more stringent quality control measures should be applied to reach higher sigma levels.", "gemini": "Lý lịch Hemoglobin A1c (HbA1c), là thành phần chính của hemoglobin glycated, đã được sử dụng trong việc theo dõi đường huyết hồi cứu trong nhiều năm và trong chẩn đoán bệnh đái tháo đường hiện nay. Vì hiệu suất phân tích của HbA1c phải cao như tất cả các xét nghiệm trong phòng xét nghiệm, nên các biện pháp kiểm soát chất lượng khác nhau được sử dụng. Sigma metrics là một trong những biện pháp này và là sự kết hợp của sai số, độ chính xác và tổng lỗi cho phép đảm bảo đánh giá chung về chất lượng phân tích. Mục đích của nghiên cứu của chúng tôi là đánh giá hiệu suất phân tích của máy phân tích HbA1c Variant Turbo II của Bio-Rad theo sigma metrics. Phương pháp Các mức sigma được tính toán bằng cách sử dụng dữ liệu thu được từ hai mức kiểm soát chất lượng nội bộ và 12 kiểm soát chất lượng bên ngoài (Bio-Rad) của máy phân tích HbA1c Variant II Turbo theo công thức σ = (TEa% - Bias%) / CV%. Kết quả Mức sigma trung bình đối với vật liệu kiểm soát chất lượng thấp và cao lần lượt là 3,0 và 4,1. Kết luận Hiệu suất phân tích trung bình hàng năm của máy phân tích HbA1c Variant II Turbo được đánh giá là chấp nhận được theo sigma metrics. Để chắc chắn về sự khác biệt về kết quả HbA1c cho thấy sự thành công hay thất bại trong điều trị nhưng không phát sinh từ sự thay đổi phân tích, người ta cho rằng nên áp dụng các biện pháp kiểm soát chất lượng nghiêm ngặt hơn để đạt được mức sigma cao hơn."} +{"idx": 1900029, "en": "Background Multiple endocrine neoplasia type 1 (MEN1) is an autosomal dominant cancer syndrome characterized by the occurrence of primary hyperparathyroidism (PHPT), pituitary adenoma (PA) and pancreatic neuroendocrine tumor (pNET). Whether the underlying mutations in MEN1 gene predict clinical presentation of affected heterozygotes or not, is still a matter of a debate. Methods Clinical and genetic analysis of 90 consecutive MEN1 patients was performed in a retrospective, single - center study. Results MEN1 mutation was found in 67 (74.4%) patients belonging to 31 different families. Twenty nine different heteozygous mutations were found, including 6 novel point mutations (W220G, 941delG, 1088del7, 1184insA, 1473del10, 1602del17) and one large deletion of exon 8. Truncating mutations predicted development of pNETs (OR=5.8, 95% CI 1.7 - 19.7%) and PHPT (OR=4.3, 95% CI 1.5 - 12.4%). Conclusions Large number of novel mutations among MEN1 patients confirmed previously reported data. PNETs and PHPT were more frequent in patients with truncating mutations.", "gemini": "Lý lịch Loại 1 của tân sinh nội tiết đa dạng (MEN1) là một hội chứng ung thư trội trên nhiễm sắc thể thường đặc trưng bởi sự xuất hiện của cường cận giáp nguyên phát (PHPT), tuyến yên (PA) và khối u thần kinh nội tiết tụy (pNET). Liệu các đột biến tiềm ẩn trong gen MEN1 có dự đoán biểu hiện lâm sàng của những người mang gen dị hợp tử bị ảnh hưởng hay không, vẫn là vấn đề gây tranh cãi. Phương pháp Phân tích lâm sàng và di truyền của 90 bệnh nhân MEN1 liên tiếp đã được thực hiện trong một nghiên cứu hồi cứu, đơn trung tâm. Kết quả Đột biến MEN1 được tìm thấy ở 67 (74,4%) bệnh nhân thuộc 31 gia đình khác nhau. Hai mươi chín đột biến dị hợp tử khác nhau đã được tìm thấy, bao gồm 6 đột biến điểm mới (W220G, 941delG, 1088del7, 1184insA, 1473del10, 1602del17) và một đoạn lớn bị mất exon 8. Các đột biến cắt cụt dự đoán sự phát triển của pNET (OR = 5,8, 95% CI 1,7 - 19,7%) và PHPT (OR = 4,3, 95% CI 1,5 - 12,4%). Kết luận Số lượng lớn các đột biến mới ở những bệnh nhân MEN1 đã xác nhận dữ liệu đã được báo cáo trước đó. PNET và PHPT thường gặp hơn ở những bệnh nhân có đột biến cắt cụt."} +{"idx": 1900030, "en": "Background Chronic hepatitis C (CHC) is a significant cause of liver related morbidity and mortality worldwide. The role of genetics in the host response to hepatitis C virus is not elucidated. Genetic variations in UGT1A1 gene are the most common cause of hereditary unconjugated hyperbilirubinemia-Gilbert syndrome. This is the first study investigating the association of UGT1A1 TA repeats promoter genotypes with the degree of liver injury, viremia and biochemical markers in CHC patients with advanced liver injury and late virological relapse. Methods Genetic testing of UGT1A1 TA repeats promoter genotypes was performed in 42 CHC patients with advanced fibrosis and cirrhosis who achieved sustained virological response and 42 healthy blood donors. CHC patients were evaluated for clinical findings, laboratory tests and imaging. Results UGT1A1*28 genotype (7/7 TA repeats) was observed in 23.8% CHC patients and 16.7% healthy controls with no significant difference in genotype frequencies (p=0.49). Pretreatment levels of ferritin and bilirubin were associated with the presence of UGT1A1*28 genotype, indicating its potential as a predictive marker. However, in our study, there was no correlation of UGT1A1*28 genotype with the degree of fibrosis or viremia. During antiviral treatment, dose reductions and treatment interruptions, as well as treatment success and occurrence of late virological relapse were not related to the presence of UGT1A1*28 genotype in CHC patients with severe liver injury. Conclusions Frequencies of UGT1A1*28 genotype are high in both Serbian CHC patients and healthy subjects. The presence of UGT1A1*28 genotype was not associated with ribavirin-related adverse effects and had no effect on long term outcome in CHC patients.", "gemini": "Bối cảnh Viêm gan C mạn tính (CHC) là nguyên nhân đáng kể gây ra bệnh tật và tử vong liên quan đến gan trên toàn thế giới. Vai trò của di truyền trong đáp ứng của vật chủ đối với vi-rút viêm gan C vẫn chưa được làm sáng tỏ. Các biến thể di truyền ở gen UGT1A1 là nguyên nhân phổ biến nhất gây tăng bilirubin máu không liên hợp di truyền - hội chứng Gilbert. Đây là nghiên cứu đầu tiên tìm hiểu mối liên quan của kiểu gen vùng khởi động lặp lại TA của UGT1A1 với mức độ tổn thương gan, tình trạng virêmi và các dấu ấn sinh hóa ở bệnh nhân CHC bị tổn thương gan tiến triển và tái phát muộn về mặt vi-rút học. Phương pháp Xét nghiệm di truyền kiểu gen vùng khởi động lặp lại TA của UGT1A1 đã được thực hiện ở 42 bệnh nhân CHC bị xơ gan tiến triển và xơ gan đã đạt được đáp ứng vi-rút bền vững và 42 người hiến máu khỏe mạnh. Bệnh nhân CHC được đánh giá các triệu chứng lâm sàng, xét nghiệm và chẩn đoán hình ảnh. Kết quả Kiểu gen UGT1A1*28 (lặp lại 7/7 TA) được quan sát thấy ở 23,8% bệnh nhân CHC và 16,7% đối chứng khỏe mạnh không có sự khác biệt đáng kể về tần số kiểu gen (p = 0,49). Mức độ ferritin và bilirubin trước điều trị có liên quan đến sự hiện diện của kiểu gen UGT1A1*28, cho thấy tiềm năng của nó như một dấu hiệu dự đoán. Tuy nhiên, trong nghiên cứu của chúng tôi, không có mối tương quan nào giữa kiểu gen UGT1A1*28 với mức độ xơ hóa hoặc tình trạng virêmi. Trong quá trình điều trị bằng thuốc kháng vi-rút, việc giảm liều và ngắt quãng điều trị, cũng như thành công của điều trị và xảy ra tái phát vi-rút muộn không liên quan đến sự hiện diện của kiểu gen UGT1A1*28 ở bệnh nhân CHC bị tổn thương gan nặng. Kết luận Tần số kiểu gen UGT1A1*28 cao ở cả bệnh nhân CHC Serbia và những người khỏe mạnh. Sự hiện diện của kiểu gen UGT1A1*28 không liên quan đến các tác dụng phụ liên quan đến ribavirin và không ảnh hưởng đến kết quả lâu dài ở bệnh nhân CHC."} +{"idx": 1900031, "en": "Background The aim of the study was to investigate the association of Cystatin C (CysC) to biochemical markers of bone turnover and bone mass, and to evaluate its prognostic significance in elderly males with chronic heart failure (CHF). Methods A prospective cohort study was executed on sixtyeight males (mean age 68±7 years) with mild to moderate CHF, together with 19 of corresponding age- and body mass index-matched healthy individuals who underwent cardio vascular, bone mineral density (BMD), and body com position assessment. Biochemical assessment of all subjects included NT-pro-BNP, parathyroid hormone (PTH), 25-hydroxy vitamin D (25(OH)D), CysC, and biochemical markers of bone turnover including osteocalcin (OC), alkaline phosphatase (ALP), β-CrossLaps (β-CTx), osteoprotegerin (OPG), and receptor activator of nuclear factor κB ligand (RANKL). Results Serum CysC was significantly increased in males with CHF in comparison to healthy control ones. A significant positive association was found between CysC levels and OC in males with CHF, while OC and β-CTx increased in increasing CysC tertiles. In multivariate regression analysis, OC and smoking were a significant determinant of CysC in males with CHF. Level of CysC was found to be positively associated with an increased fatal risk in males with CHF. Conclusions Serum osteocalcin is an independent predictor of CysC level in elderly males with CHF. Higher CysC level showed a negative relation to survival and bone loss in males with CHF. Further research is needed to confirm the potential role of CysC in the crosstalk between heart, kidney, bone, and energy metabolism in CHF.", "gemini": "Bối cảnh Mục đích của nghiên cứu là điều tra mối liên quan của Cystatin C (CysC) với các dấu ấn sinh hóa về sự chuyển hóa xương và khối lượng xương, và đánh giá ý nghĩa tiên lượng của nó ở nam giới cao tuổi bị suy tim mạn tính (CHF). Phương pháp Một nghiên cứu theo dõi triển vọng đã được thực hiện trên sáu mươi tám nam giới (tuổi trung bình 68 ± 7) bị CHF nhẹ đến trung bình, cùng với 19 người có cùng độ tuổi và chỉ số khối cơ thể phù hợp với những người khỏe mạnh đã trải qua đánh giá tim mạch, mật độ khoáng xương (BMD) và thành phần cơ thể. Đánh giá sinh hóa của tất cả các đối tượng bao gồm NT-pro-BNP, hormone tuyến cận giáp (PTH), vitamin D 25-hydroxy (25 (OH) D), CysC và các dấu ấn sinh hóa về sự chuyển hóa xương bao gồm osteocalcin (OC), phosphatase kiềm (ALP), β-CrossLaps (β-CTx), osteoprotegerin (OPG) và thụ thể kích hoạt của nhân tố hạt nhân κB phối tử (RANKL). Kết quả CysC huyết thanh tăng đáng kể ở nam giới bị CHF so với những người đối chứng khỏe mạnh. Một mối liên quan tích cực đáng kể được tìm thấy giữa nồng độ CysC và OC ở nam giới bị CHF, trong khi OC và β-CTx tăng ở các phần ba CysC tăng. Trong phân tích hồi quy đa biến, OC và hút thuốc là yếu tố quyết định đáng kể của CysC ở nam giới bị CHF. Mức độ CysC được phát hiện có liên quan tích cực với nguy cơ tử vong tăng ở nam giới bị CHF. Kết luận Osteocalcin huyết thanh là một yếu tố dự đoán độc lập về mức độ CysC ở nam giới cao tuổi bị CHF. Nồng độ CysC cao hơn cho thấy mối quan hệ tiêu cực với sự sống sót và mất xương ở nam giới bị CHF. Cần nghiên cứu thêm để xác nhận vai trò tiềm tàng của CysC trong sự tương tác giữa tim, thận, xương và chuyển hóa năng lượng ở CHF."} +{"idx": 1900032, "en": "Background Triple negative breast cancer (TNBC) is characterized by aggressive clinical course and is unresponsive to anti-HER2 and endocrine therapy. TNBC is difficult to treat and is often lethal. Given the need to find new targets for therapy we explored clinicopathological significance of copy number gain of FGFR1 and c-MYC. Our aim was to determine the impact of FGFR1 and c-MYC copy number gain on clinical course and outcome of TNBC. Methods FGFR1 and c-MYC gene copy number alterations were evaluated in 78 archive TNBC samples using TaqMan based quantitative real time PCR assays. Results 50% of samples had increased c-MYC copy number. c-MYC copy number gain was associated with TNBC in contrast to ER positive cancers. Our results showed significant correlation between c-MYC copy number gain and high grade of TNBCs. This suggests that c-MYC copy number could be an useful prognostic marker for TNBC patients. c-MYC copy number gain was associated with high pTNM stage as well as lobular and medullary tumor subtypes. 43% of samples had increased FGFR1 copy number. No correlations between FGFR1 copy number gain and clinicopathological variables were observed. Conclusions We identified c-MYC copy number gain as a prognostic marker for TNBC. Our results indicate that c- MYC may contribute to TNBC progression. We observed no significant association between c-MYC and/or FGFR1 copy number status and patient survival.", "gemini": "Bối cảnh Ung thư vú ba âm tính (TNBC) được đặc trưng bởi diễn biến lâm sàng hung dữ và không đáp ứng với liệu pháp nội tiết và kháng HER2. TNBC rất khó điều trị và thường gây tử vong. Do nhu cầu tìm ra các mục tiêu mới để điều trị, chúng tôi đã khám phá ý nghĩa lâm sàng của việc tăng số bản sao của FGFR1 và c-MYC. Mục đích của chúng tôi là xác định tác động của việc tăng số bản sao FGFR1 và c-MYC lên diễn biến lâm sàng và kết cục của TNBC. Phương pháp Các biến đổi số bản sao gen FGFR1 và c-MYC đã được đánh giá ở 78 mẫu TNBC lưu trữ bằng xét nghiệm PCR định lượng thời gian thực dựa trên TaqMan. Kết quả 50% mẫu có số bản sao c-MYC tăng lên. Việc tăng số bản sao c-MYC có liên quan đến TNBC trái ngược với các loại ung thư dương tính với ER. Kết quả của chúng tôi cho thấy có mối tương quan đáng kể giữa việc tăng số bản sao c-MYC và mức độ TNBC cao. Điều này cho thấy rằng số bản sao c-MYC có thể là một dấu hiệu tiên lượng hữu ích cho bệnh nhân TNBC. Việc tăng số bản sao c-MYC có liên quan đến giai đoạn pTNM cao cũng như các phân nhóm u tiểu thùy và tủy. 43% mẫu có số bản sao FGFR1 tăng lên. Không có mối tương quan nào giữa việc tăng số bản sao FGFR1 và các biến lâm sàng được quan sát thấy. Kết luận Chúng tôi xác định việc tăng số bản sao c-MYC là một dấu hiệu tiên lượng cho TNBC. Kết quả của chúng tôi chỉ ra rằng c-MYC có thể góp phần vào sự tiến triển của TNBC. Chúng tôi không quan sát thấy mối liên quan đáng kể nào giữa tình trạng số bản sao c-MYC và/hoặc FGFR1 và sự sống còn của bệnh nhân."} +{"idx": 1900033, "en": "Background Gestational hypertension (GH) and pre eclampsia (PE) are the most common gestational complications. Several placental biochemical markers are used to predict GH/PE, but with conflicting results. Methods The study aim was to estimate the biochemical markers' ability to predict hypertensive disorders in pregnancy. On the first ultrasonographic examination, 104 healthy pregnant women were recruited. At the regular pregnancy check-ups, BMI, blood pressure, occurrence of gestational hypertension (early or late onset), preeclampsia, eclampsia and other complications were recorded. Serum concentrations (in multiples of median - MoM) of human chorionic gonadotropin (HCG) and pregnancyassociated plasma protein A (PAPPA) were measured from the 11th to 14th gestational week, while HCG, alpha feto protein (AFP), estriol and inhibin were determined between the 16th and 19th gestational week. Results Hypertensive disorders throughout pregnancy were diagnosed in 20.2% women. Early-onset GH was registered in 7 and PE in 6 patients, while 14 had late-onset GH and 10 additional women PE. There were no significant differences (p≥0.05) in biochemical markers concentrations between women with and without GH/PE. PAPPA levels in the first and HCG in the second trimester correlated with early and late GH/PE. Moreover, higher AFP concentrations were registered in women with preeclampsia signs/symptoms. According to ROC analysis, AFP>1.05 MoM properly identified 80% of GH/PE cases. Obtained models imply that HCG, PAPPA and AFP should be used for GH/PE prediction. Conclusions Biochemical markers HCG, PAPPA and AFP could be useful in predicting gestational hypertension and preeclampsia. However, different markers should be used for early and late onset GH/PE.", "gemini": "Tiền căn Tăng huyết áp thai kỳ (GH) và tiền sản giật (PE) là những biến chứng thai kỳ phổ biến nhất. Một số dấu ấn sinh hóa nhau thai được sử dụng để dự đoán GH/PE, nhưng cho kết quả trái ngược nhau. Phương pháp Mục đích của nghiên cứu là ước tính khả năng dự đoán các rối loạn tăng huyết áp trong thai kỳ của các dấu ấn sinh hóa. Trong lần siêu âm đầu tiên, 104 phụ nữ mang thai khỏe mạnh đã được tuyển dụng. Tại các lần kiểm tra thai kỳ định kỳ, BMI, huyết áp, tình trạng tăng huyết áp thai kỳ (bắt đầu sớm hoặc muộn), tiền sản giật, sản giật và các biến chứng khác đã được ghi lại. Nồng độ huyết thanh (gấp nhiều lần trung vị - MoM) của gonadotropin màng đệm ở người (HCG) và protein huyết tương liên quan đến thai kỳ A (PAPPA) được đo từ tuần thai thứ 11 đến tuần thứ 14, trong khi HCG, protein thai nhi alpha (AFP), estriol và inhibin được xác định giữa tuần thai thứ 16 và tuần thứ 19. Kết quả Các rối loạn tăng huyết áp trong suốt thai kỳ được chẩn đoán ở 20,2% phụ nữ. GH khởi phát sớm được ghi nhận ở 7 bệnh nhân và PE ở 6 bệnh nhân, trong khi 14 bệnh nhân bị GH khởi phát muộn và 10 phụ nữ khác bị PE. Không có sự khác biệt đáng kể (p≥0,05) về nồng độ dấu ấn sinh hóa giữa những phụ nữ mắc GH/PE và không mắc GH/PE. Nồng độ PAPPA trong tam cá nguyệt đầu tiên và HCG trong tam cá nguyệt thứ hai tương quan với GH/PE sớm và muộn. Hơn nữa, nồng độ AFP cao hơn được ghi nhận ở những phụ nữ có các dấu hiệu/triệu chứng tiền sản giật. Theo phân tích ROC, AFP>1,05 MoM xác định đúng 80% các trường hợp GH/PE. Các mô hình thu được ngụ ý rằng HCG, PAPPA và AFP nên được sử dụng để dự đoán GH/PE. Kết luận Các dấu ấn sinh hóa HCG, PAPPA và AFP có thể hữu ích trong việc dự đoán tăng huyết áp thai kỳ và tiền sản giật. Tuy nhiên, nên sử dụng các dấu ấn khác nhau cho GH/PE khởi phát sớm và muộn."} +{"idx": 1900034, "en": "Background Radiation therapy remains an important therapeutic modality, especially for those patients who are not candidates for radical resection. Many strategies have been developed to increase the radiosensitivity of esophageal cancer, with some success. Methods This study was conducted to determine whether raltitrexed can enhance radiosensitivity of esophageal squamous cell carcinoma (ESCC). ESCC cell lines 24 h were incubated with raltitrexed or DMSO with or without subsequent irradiation. Cell Counting Kit assay-8 assay and clonogenic survival assay were used to measure the cell proliferation and radiosensitization, respectively. Flow cytometry was utilized to examine cell apoptosis and cell cycle distribution in different groups. Immunofluorescence analysis was performed to detect deoxyribonucleic acid (DNA) double-strand breaks. In addition, the expression levels of proteins that are involved in radiation induced signal transduction including Bax, Cyclin B1, Cdc2/pCdc2, and Cdc25C/pCdc25C were examined by western blot analysis. Results The results indicated that raltitrexed enhanced radiosensitivity of ESCC cells with increased DNA double-strand breaks, the G2/M arrest, and the apoptosis of ESCC cells induced by radiation. The sensitization enhancement ratio of 1.23-2.10 was detected for ESCC cells with raltitrexed treatment in TE-13 cell line. In vitro, raltitrexed also increased the therapeutic effect of radiation in nude mice. Conclusion Raltitrexed increases the radiosensitivity of ESCC. This antimetabolite drug is promising for future clinical trials with concurrent radiation in esophageal cancer.", "gemini": "Tiền căn Xạ trị vẫn là một phương thức điều trị quan trọng, đặc biệt đối với những bệnh nhân không đủ điều kiện để cắt bỏ triệt căn. Nhiều chiến lược đã được phát triển để tăng độ nhạy xạ với bức xạ của ung thư thực quản, với một số thành công. Phương pháp Nghiên cứu này được tiến hành để xác định xem raltitrexed có thể tăng độ nhạy xạ với bức xạ của ung thư biểu mô tế bào vảy thực quản (ESCC) hay không. Các dòng tế bào ESCC 24 giờ được ủ với raltitrexed hoặc DMSO có hoặc không có chiếu xạ sau đó. Đánh giá bộ đếm tế bào Kit-8 và đánh giá khả năng sinh khối u được sử dụng để đo sự tăng sinh tế bào và độ nhạy xạ với bức xạ, tương ứng. Phân tích dòng chảy tế bào được sử dụng để kiểm tra quá trình chết theo chương trình của tế bào và phân bố chu kỳ tế bào trong các nhóm khác nhau. Phân tích miễn dịch huỳnh quang được thực hiện để phát hiện các đứt gãy sợi đôi của axit deoxyribonucleic (DNA). Ngoài ra, mức độ biểu hiện của các protein liên quan đến quá trình truyền tín hiệu do bức xạ gây ra bao gồm Bax, Cyclin B1, Cdc2/pCdc2 và Cdc25C/pCdc25C đã được kiểm tra bằng phương pháp phân tích vết hấp thụ. Kết quả Kết quả cho thấy raltitrexed làm tăng độ nhạy xạ với bức xạ của các tế bào ESCC với sự gia tăng các đứt gãy sợi đôi của DNA, sự bắt giữ G2/M và quá trình chết theo chương trình của các tế bào ESCC do bức xạ gây ra. Tỷ lệ tăng cường độ nhạy cảm từ 1,23-2,10 được phát hiện đối với các tế bào ESCC được điều trị bằng raltitrexed trong dòng tế bào TE-13. Trong ống nghiệm, raltitrexed cũng làm tăng hiệu quả điều trị của bức xạ ở chuột khỏa thân. Kết luận Raltitrexed làm tăng độ nhạy xạ với bức xạ của ESCC. Thuốc chống chuyển hóa này hứa hẹn cho các thử nghiệm lâm sàng trong tương lai với bức xạ đồng thời trong ung thư thực quản."} +{"idx": 1900035, "en": "Background Work-family conflict (WFC) is a crucial problem in nursing because of the demanding conditions of the job, such as strenuous shifts, physical and emotional workload, and intense patient involvement. Using a multilevel approach, this study investigated the moderating role of collective affective commitment as a protective resource in the relationship between WFC and emotional exhaustion. Methods The sample included 647 nurses from 66 working units in 4 Italian hospitals. A self-administrated questionnaire was administered to nurses. To analyze data, hierarchical linear modeling was used to examine cross-level relationships between variables. Results The results indicated that emotional exhaustion increased with augmenting of WFC and that this relationship was stronger when collective affective commitment was low and weaker when it was high. Conclusions The study thus suggests that collective affective commitment may be considered a protective resource for nurses. Moreover, the results show that high work-family conflict should not represent a serious problem when nurses have high affective commitment. Interventions at both individual and group level are discussed in order to mitigate WFC, promoting collective affective commitment and thus reducing emotional exhaustion.", "gemini": "Tiền căn Xung đột công việc - gia đình (WFC) là một vấn đề quan trọng trong điều dưỡng vì những điều kiện đòi hỏi của công việc, chẳng hạn như ca làm việc căng thẳng, khối lượng công việc thể chất và tinh thần và sự tham gia của bệnh nhân rất nhiều. Sử dụng phương pháp tiếp cận đa cấp, nghiên cứu này đã điều tra vai trò điều chỉnh của cam kết tình cảm tập thể như một nguồn lực bảo vệ trong mối quan hệ giữa WFC và kiệt sức về mặt cảm xúc. Phương pháp Mẫu nghiên cứu bao gồm 647 y tá từ 66 đơn vị làm việc tại 4 bệnh viện ở Ý. Một bảng câu hỏi tự quản đã được tiến hành đối với các y tá. Để phân tích dữ liệu, mô hình tuyến tính phân cấp đã được sử dụng để kiểm tra các mối quan hệ giữa các cấp độ giữa các biến. Kết quả Kết quả cho thấy kiệt sức về mặt cảm xúc tăng lên khi WFC tăng và mối quan hệ này mạnh hơn khi cam kết tình cảm tập thể thấp và yếu hơn khi cam kết tình cảm tập thể cao. Kết luận Do đó, nghiên cứu cho thấy cam kết tình cảm tập thể có thể được coi là một nguồn lực bảo vệ cho các y tá. Hơn nữa, kết quả cho thấy xung đột công việc - gia đình cao không nên là một vấn đề nghiêm trọng khi các y tá có cam kết tình cảm cao. Các biện pháp can thiệp ở cả cấp độ cá nhân và nhóm được thảo luận để giảm thiểu WFC, thúc đẩy cam kết tình cảm tập thể và do đó giảm kiệt sức về mặt cảm xúc."} +{"idx": 1900036, "en": "Background The staging system of nasopharyngeal carcinoma (NPC) has close relationship with the degree of cell differentiation, but most NPC patients remain undiagnosed until advanced phases. Novel metabolic markers need to be characterized to support diagnose at an early stage. Methods Metabolic characteristics of nasopharyngeal normal cell NP69 and two types of NPC cells, including CNE1 and CNE2 associated with high and low differentiation degrees were studied by combining 1H NMR spectroscopy with Raman spectroscopy. Statistical methods were also utilized to determine potential characteristic metabolites for monitoring differentiation progression. Results Metabolic profiles of NPC cells were significantly different according to differentiation degrees. Various characteristic metabolites responsible for different differentiated NPC cells were identified, and then disordered metabolic pathways were combed according to these metabolites. We found disordered pathways mainly included amino acids metabolisms like essential amino acids metabolisms, as well as altered lipid metabolism and TCA cycle, and abnormal energy metabolism. Thus our results provide evidence about close relationship between differentiation degrees of NPC cells and the levels of intracellular metabolites. Moreover, Raman spectrum analysis also provided complementary and confirmatory information about intracellular components in single living cells. Eight pathways were verified to that in NMR analysis, including amino acids metabolisms, inositol phosphate metabolism, and purine metabolism. Conclusions Methodology of NMR-based metabolomics combining with Raman spectroscopy could be powerful and straightforward to reveal cell differentiation development and meanwhile lay the basis for experimental and clinical practice to monitor disease progression and therapeutic evaluation.", "gemini": "Bối cảnh Hệ thống phân giai của ung thư vòm họng (NPC) có mối quan hệ chặt chẽ với mức độ biệt hóa tế bào, nhưng hầu hết bệnh nhân NPC vẫn chưa được chẩn đoán cho đến khi ở giai đoạn tiến triển. Các dấu ấn chuyển hóa mới cần được đặc trưng để hỗ trợ chẩn đoán ở giai đoạn sớm. Phương pháp Các đặc điểm chuyển hóa của tế bào bình thường vòm họng NP69 và hai loại tế bào NPC, bao gồm CNE1 và CNE2 liên quan đến mức độ biệt hóa cao và thấp đã được nghiên cứu bằng cách kết hợp phổ NMR 1H với phổ Raman. Các phương pháp thống kê cũng được sử dụng để xác định các chất chuyển hóa đặc trưng tiềm năng để theo dõi quá trình biệt hóa. Kết quả Hồ sơ chuyển hóa của các tế bào NPC khác nhau đáng kể tùy theo mức độ biệt hóa. Nhiều chất chuyển hóa đặc trưng chịu trách nhiệm cho các tế bào NPC biệt hóa khác nhau đã được xác định, và sau đó các con đường chuyển hóa rối loạn đã được chải theo các chất chuyển hóa này. Chúng tôi thấy rằng các con đường rối loạn chủ yếu bao gồm quá trình chuyển hóa axit amin như quá trình chuyển hóa axit amin thiết yếu, cũng như quá trình chuyển hóa lipid và chu trình TCA bị thay đổi và quá trình chuyển hóa năng lượng bất thường. Do đó, kết quả của chúng tôi cung cấp bằng chứng về mối quan hệ chặt chẽ giữa mức độ biệt hóa của các tế bào NPC và mức độ chất chuyển hóa trong tế bào. Hơn nữa, phân tích phổ Raman cũng cung cấp thông tin bổ sung và xác nhận về các thành phần trong tế bào ở các tế bào sống đơn lẻ. Tám con đường đã được xác minh trong phân tích NMR, bao gồm quá trình chuyển hóa axit amin, quá trình chuyển hóa inositol phosphat và quá trình chuyển hóa purin. Kết luận Phương pháp chuyển hóa học dựa trên NMR kết hợp với phổ Raman có thể mạnh mẽ và đơn giản để tiết lộ sự phát triển biệt hóa tế bào và đồng thời đặt cơ sở cho thực hành thực nghiệm và lâm sàng để theo dõi tiến triển bệnh và đánh giá hiệu quả điều trị."} +{"idx": 1900037, "en": "Wildlife poisoning is an important conservation threat for endangered species in India. There are no publications in the scientific literature that identify the specific poisons or chemicals involved in wildlife poisoning cases from the state of Kerala. In this report, all cases of wildlife mortality recorded between 2011 and 2013 at the office of the Assistant Forest Veterinary Officer, Periyar Tiger Reserve in Kerala were reviewed and cases where poisoning was considered as a differential diagnosis were identified. Specific poisons or chemicals were identified in three cases, while in a fourth, poisoning was determined to have occurred based on physical traces of the poison in gut contents. The poisons identified include carbofuran (a carbamate pesticide) in a bonnet macaque (Macaca radiata), warfarin (a rodenticide) in a mortality event involving four wild boars (Sus scrofa), endosulfan (an organochlorine pesticide) toxicity in a gaur (Bos gaurus) and imidacloprid (a neonicotinoid pesticide) toxicity in a wild adult Asian elephant (Elephas maximus). This communication thus reports for the first time on the specific chemical compounds identified in wildlife poisoning cases from Kerala state and argues for greater regulation of the sale and use of such toxic compounds in India.", "gemini": "Ngộ độc động vật hoang dã là mối đe dọa bảo tồn quan trọng đối với các loài có nguy cơ tuyệt chủng ở Ấn Độ. Không có ấn phẩm nào trong tài liệu khoa học xác định các chất độc hoặc hóa chất cụ thể liên quan đến các trường hợp ngộ độc động vật hoang dã từ tiểu bang Kerala. Trong báo cáo này, tất cả các trường hợp tử vong của động vật hoang dã được ghi nhận từ năm 2011 đến năm 2013 tại văn phòng của Trợ lý bác sĩ thú y lâm nghiệp, Khu bảo tồn hổ Periyar ở Kerala đã được xem xét và các trường hợp ngộ độc được coi là chẩn đoán phân biệt đã được xác định. Các chất độc hoặc hóa chất cụ thể đã được xác định trong ba trường hợp, trong khi ở trường hợp thứ tư, ngộ độc được xác định là đã xảy ra dựa trên các dấu vết vật lý của chất độc trong ruột. Các chất độc được xác định bao gồm carbofuran (thuốc trừ sâu carbamate) ở một con khỉ mũ trùm đầu (Macaca radiata), warfarin (thuốc diệt chuột) trong một sự kiện tử vong liên quan đến bốn con lợn rừng (Sus scrofa), độc tính endosulfan (thuốc trừ sâu organochlorine) ở một con bò gaur (Bos gaurus) và độc tính imidacloprid (thuốc trừ sâu neonicotinoid) ở một con voi châu Á trưởng thành hoang dã (Elephas maximus). Do đó, thông tin này lần đầu tiên báo cáo về các hợp chất hóa học cụ thể được xác định trong các trường hợp ngộ độc động vật hoang dã từ tiểu bang Kerala và lập luận rằng cần tăng cường kiểm soát việc buôn bán và sử dụng các hợp chất độc hại như vậy ở Ấn Độ."} +{"idx": 1900038, "en": "Integrated knowledge translation (IKT) is built upon the premise that involving knowledge users as partners in the research process will result in science that is more relevant to the public and therefore will have greater impact. Drawing on our experiences with a large and multifaceted IKT food allergy research program we highlight the disjuncture between the goals of IKT and the nature of basic science research, most notably the long timelines before research is ready for translation. Our partner consultations concluded that IKT success should be measured in a different way. That is, it should not be about informing an immediate gap in the translation of food allergy findings but about building relationships between our partners, greater awareness, understanding and knowledge about the nature of science and IKT, and ultimately helping to create better policy and science down the road. It is the recognition that it behooves us as scientists to be able to answer those \"why\" questions. We call for other researchers to consider the success of IKT beyond the short term timelines of any one research project but instead as an avenue to build partnerships, innovate thinking about research questions and to maximize choice and minimize risk for individuals in Canada and beyond affected by food allergy.", "gemini": "Bản dịch kiến thức tích hợp (IKT) được xây dựng dựa trên tiền đề rằng việc liên quan đến người sử dụng kiến thức như là đối tác trong quá trình nghiên cứu sẽ dẫn đến khoa học có liên quan hơn đến công chúng và do đó sẽ có tác động lớn hơn. Dựa trên kinh nghiệm của chúng tôi với một chương trình nghiên cứu dị ứng thực phẩm IKT đa dạng và lớn, chúng tôi nhấn mạnh sự khác biệt giữa các mục tiêu của IKT và bản chất của nghiên cứu khoa học cơ bản, đáng chú ý nhất là mốc thời gian dài trước khi nghiên cứu sẵn sàng để chuyển dịch. Các cuộc tham vấn đối tác của chúng tôi kết luận rằng thành công của IKT nên được đo lường theo một cách khác. Nghĩa là, nó không nên chỉ là thông báo về khoảng cách ngay lập tức trong việc chuyển dịch các phát hiện về dị ứng thực phẩm mà còn là xây dựng mối quan hệ giữa các đối tác của chúng tôi, nâng cao nhận thức, hiểu biết và kiến thức về bản chất của khoa học và IKT và cuối cùng là giúp tạo ra chính sách và khoa học tốt hơn trong tương lai. Đó là sự thừa nhận rằng chúng tôi là những nhà khoa học có trách nhiệm trả lời những câu hỏi \"tại sao\" đó. Chúng tôi kêu gọi các nhà nghiên cứu khác xem xét sự thành công của IKT vượt ra ngoài mốc thời gian ngắn hạn của bất kỳ dự án nghiên cứu nào mà thay vào đó là một con đường để xây dựng quan hệ đối tác, đổi mới suy nghĩ về các câu hỏi nghiên cứu và tối đa hóa sự lựa chọn và giảm thiểu rủi ro cho các cá nhân ở Canada và những nơi khác bị ảnh hưởng bởi dị ứng thực phẩm."} +{"idx": 1900039, "en": "Objective Tetrahydroxy stilbene glucoside (TSG) has been reported to exert a cytoprotective effect against various toxicants. However, the function and mechanism of TSG in palmitic acid (PA)-induced inflammation and apoptosis in cardiomyocytes are still unknown. The present study was designed to investigate the post-transcriptional mechanism in TSG-treated cardiomyocytes' inflammation and apoptosis induced by PA. Methods The mRNA and protein levels were assayed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting, respectively. The targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. Cell proliferation was analyzed by CCK-8 assay. Annexin V-fluorescein isothiocyanate/polyimide (annexin V-FITC/PI) staining was used to evaluate apoptosis using flow cytometry. Results TSG restricted the detrimental effects, including the activated inflammatory response and apoptosis, of PA in cardiomyocytes, as well as the up-regulation of miR-129-3p and down-regulation of p-Smad3 expression. In addition, bioinformatics and experimental analysis suggested that Smad3 was a direct target of miR-129-3p, which could inhibit or enhance the expression of p-Smad by transfection with miR-129-3p mimics or inhibitors, respectively. Furthermore, our results demonstrated that overexpression of Smad3 reversed the inhibition of inflammation and apoptosis by overexpression of miR-129-3p in PA-stimulated cardiomyocytes. Conclusion TSG targeted to miR-129-3p/Smad3 signaling inhibited PA-induced inflammation and apoptosis in cardiomyocytes.", "gemini": "Mục tiêu Tetrahydroxy stilbene glucoside (TSG) đã được báo cáo là có tác dụng bảo vệ tế bào chống lại nhiều chất độc khác nhau. Tuy nhiên, chức năng và cơ chế của TSG trong tình trạng viêm và apoptosis do axit palmitic (PA) gây ra ở tế bào cơ tim vẫn chưa được biết rõ. Nghiên cứu hiện tại được thiết kế để tìm hiểu cơ chế hậu phiên mã trong tình trạng viêm và apoptosis của tế bào cơ tim được điều trị bằng TSG do PA gây ra. Phương pháp Nồng độ mRNA và protein được phân tích bằng phản ứng chuỗi polymerase định lượng phiên mã ngược (RT-qPCR) và phương pháp in ấn chuyển phương Tây, tương ứng. Các gen mục tiêu được dự đoán bằng thuật toán tin sinh học và được xác nhận bằng xét nghiệm báo cáo kép luciferase. Sự tăng sinh tế bào được phân tích bằng xét nghiệm CCK-8. Phương pháp nhuộm annexin V-fluorescein isothiocyanate/polyimide (annexin V-FITC/PI) được sử dụng để đánh giá apoptosis bằng phương pháp đo lưu lượng tế bào. Kết quả TSG hạn chế các tác động có hại, bao gồm cả phản ứng viêm được kích hoạt và apoptosis của PA trong tế bào cơ tim, cũng như sự điều hòa ngược dòng của miR-129-3p và điều hòa giảm dòng của biểu hiện p-Smad3. Ngoài ra, phân tích tin sinh học và thực nghiệm cho thấy Smad3 là mục tiêu trực tiếp của miR-129-3p, có thể ức chế hoặc tăng cường biểu hiện của p-Smad bằng cách chuyển gen với các chất bắt chước miR-129-3p hoặc chất ức chế, tương ứng. Hơn nữa, kết quả của chúng tôi chứng minh rằng việc biểu hiện quá mức Smad3 đảo ngược sự ức chế viêm và apoptosis bằng cách biểu hiện quá mức miR-129-3p ở các tế bào cơ tim được kích thích bằng PA. Kết luận TSG nhắm vào tín hiệu miR-129-3p/Smad3 ức chế tình trạng viêm và apoptosis do PA gây ra ở tế bào cơ tim."} +{"idx": 1900040, "en": "Background Polyethylene glycols (PEGs) and their derivatives are non-ionic polymers of ethylene oxide commercially available with numerous synonyms, such as macrogol, oxyethylene polymer, and laureth-9. Although these polymers are usually safe, mild to life-threatening immediate-type hypersensitivity reactions have been reported. Nevertheless, awareness about their allergic potential is minimal due to the non-standardization of their nomenclature. Case presentation We present the case of a 29-years-old woman who developed several local and systemic type I hypersensitivity reactions including a severe anaphylactic reaction to different pharmacologic and cosmetic products whose excipients included PEG. Prick tests and basophil activation tests were performed to several pharmacological and cosmetic products, but only those containing PEGs and their derivatives were positive. The patient was diagnosed with immediate hypersensitivity IgE-mediated to PEGs and its derivatives. Conclusions Standardization of the terminology used to describe the presence of PEGs in products would help patients to identify them clearly and unequivocally and thus avoid the development of hypersensitivity reactions. It is also recommended studying PEG allergy in reactions to products containing PEGs, once allergy to the active ingredients has been excluded and in reactions to multiple unrelated drugs.Clinical study protocol number PI2018/29 (registered on 24 September 2018).", "gemini": "Bối cảnh Polyethylene glycol (PEG) và các dẫn xuất của chúng là các polyme không ion của oxit etylen có sẵn trên thị trường với nhiều từ đồng nghĩa, chẳng hạn như macrogol, polyme oxyethylene và laureth-9. Mặc dù các polyme này thường an toàn, nhưng vẫn có báo cáo về các phản ứng quá mẫn loại tức thời nhẹ đến đe dọa tính mạng. Tuy nhiên, nhận thức về khả năng gây dị ứng của chúng là rất ít do tên gọi của chúng không được chuẩn hóa. Trình bày ca bệnh Chúng tôi trình bày trường hợp của một phụ nữ 29 tuổi, người đã phát triển một số phản ứng quá mẫn loại I tại chỗ và toàn thân bao gồm phản ứng phản vệ nghiêm trọng đối với các sản phẩm dược lý và mỹ phẩm khác nhau có tá dược bao gồm PEG. Các xét nghiệm chích da và xét nghiệm hoạt hóa tế bào mast đã được thực hiện đối với một số sản phẩm dược lý và mỹ phẩm, nhưng chỉ những sản phẩm có chứa PEG và các dẫn xuất của chúng mới có kết quả dương tính. Bệnh nhân được chẩn đoán là quá mẫn tức thời qua trung gian IgE đối với PEG và các dẫn xuất của chúng. Kết luận Việc chuẩn hóa thuật ngữ được sử dụng để mô tả sự hiện diện của PEG trong các sản phẩm sẽ giúp bệnh nhân xác định chúng một cách rõ ràng và chắc chắn, do đó tránh được sự phát triển của các phản ứng quá mẫn. Cũng nên nghiên cứu dị ứng PEG trong các phản ứng với các sản phẩm có chứa PEG, sau khi đã loại trừ dị ứng với các thành phần hoạt tính và trong các phản ứng với nhiều loại thuốc không liên quan.Số giao thức nghiên cứu lâm sàng PI2018/29 (đăng ký ngày 24 tháng 9 năm 2018)."} +{"idx": 1900041, "en": "Aim To study the dosimetric impact of statistical uncertainty (SU) per plan on Monte Carlo (MC) calculation in Monaco™ treatment planning system (TPS) during volumetric modulated arc therapy (VMAT) for three different clinical cases. Background During MC calculation SU is an important factor to decide dose calculation accuracy and calculation time. It is necessary to evaluate optimal acceptance of SU for quality plan with reduced calculation time. Materials and methods Three different clinical cases as the lung, larynx, and prostate treated using VMAT technique were chosen. Plans were generated with Monaco™ V5.11 TPS with 2% statistical uncertainty. By keeping all other parameters constant, plans were recalculated by varying SU, 0.5%, 1%, 2%, 3%, 4%, and 5%. For plan evaluation, conformity index (CI), homogeneity index (HI), dose coverage to PTV, organ at risk (OAR) dose, normal tissue receiving dose ≥5 Gy and ≥10 Gy, integral dose (NTID), calculation time, gamma pass rate, calculation reproducibility and energy dependency were analyzed. Results CI and HI improve as SU increases from 0.5% to 5%. No significant dose difference was observed in dose coverage to PTV, OAR doses, normal tissue receiving dose ≥5 Gy and ≥10 Gy and NTID. Increase of SU showed decrease in calculation time, gamma pass rate and increase in PTV max dose. No dose difference was seen in calculation reproducibility and dependent on energy. Conclusion For VMAT plans, SU can be accepted from 1% to 3% per plan with reduced calculation time without compromising plan quality and deliverability by accepting variations in point dose within the target.", "gemini": "Mục đích Nghiên cứu tác động về liều lượng của độ không chắc chắn thống kê (SU) trên mỗi kế hoạch về tính toán Monte Carlo (MC) trong hệ thống lập kế hoạch điều trị Monaco™ (TPS) trong quá trình điều trị vòng cung điều biến thể tích (VMAT) cho ba trường hợp lâm sàng khác nhau. Bối cảnh Trong quá trình tính toán MC, SU là một yếu tố quan trọng để quyết định độ chính xác của tính toán liều lượng và thời gian tính toán. Cần phải đánh giá sự chấp nhận tối ưu đối với SU đối với kế hoạch chất lượng với thời gian tính toán được giảm. Vật liệu và phương pháp Ba trường hợp lâm sàng khác nhau như phổi, thanh quản và tuyến tiền liệt được điều trị bằng kỹ thuật VMAT đã được chọn. Các kế hoạch được tạo bằng Monaco™ V5.11 TPS với độ không chắc chắn thống kê là 2%. Bằng cách giữ tất cả các thông số khác không đổi, các kế hoạch đã được tính toán lại bằng cách thay đổi SU, 0,5%, 1%, 2%, 3%, 4% và 5%. Để đánh giá kế hoạch, chỉ số phù hợp (CI), chỉ số đồng nhất (HI), phạm vi liều lượng đến PTV, liều lượng của cơ quan có nguy cơ (OAR), mô bình thường nhận liều lượng ≥5 Gy và ≥10 Gy, liều lượng tích phân (NTID), thời gian tính toán, tỷ lệ vượt qua gamma, khả năng tái tạo tính toán và phụ thuộc năng lượng đã được phân tích. Kết quả CI và HI cải thiện khi SU tăng từ 0,5% đến 5%. Không có sự khác biệt đáng kể về liều lượng trong phạm vi liều lượng đến PTV, liều lượng OAR, mô bình thường nhận liều lượng ≥5 Gy và ≥10 Gy và NTID. Sự gia tăng SU cho thấy thời gian tính toán giảm, tỷ lệ vượt qua gamma và liều lượng tối đa của PTV tăng lên. Không thấy sự khác biệt về liều lượng trong khả năng tái tạo tính toán và phụ thuộc vào năng lượng. Kết luận Đối với các kế hoạch VMAT, SU có thể được chấp nhận từ 1% đến 3% trên mỗi kế hoạch với thời gian tính toán giảm mà không ảnh hưởng đến chất lượng kế hoạch và khả năng phân phối bằng cách chấp nhận các biến thể về liều lượng tại điểm trong mục tiêu."} +{"idx": 1900042, "en": "Induced pluripotent stem cells derived from normal somatic cells could be utilized to study tumorigenesis through overexpression of specific oncogenes, downregulation of tumor suppressors and dysregulation of other factors thought to promote tumorigenesis. Therefore, effective approaches that provide direct modifications of induced pluripotent stem cell genome are extremely needed. Emerging strategies are expected to provide the ability to more effectively introduce diverse genetic alterations, from as small as single-nucleotide modifications to whole gene amplification or deletion, all with a high degree of target specificity. To date, several techniques have been applied in stem cell studies to directly edit cell genome (ZFNs, TALENs or CRISPR/Cas9). In this review, we summarize specific gene delivery strategies that were applied to stem cell studies together with genome editing techniques, which enable a direct modification of endogenous DNA sequences in the context of cancer studies.", "gemini": "Các tế bào gốc đa năng cảm ứng được tạo ra từ các tế bào soma bình thường có thể được sử dụng để nghiên cứu quá trình sinh khối u thông qua sự biểu hiện quá mức của các oncogen đặc hiệu, sự điều hòa giảm các chất ức chế khối u và sự điều hòa sai lệch của các yếu tố khác được cho là thúc đẩy quá trình sinh khối u. Do đó, các phương pháp tiếp cận hiệu quả cung cấp các sửa đổi trực tiếp đối với bộ gen tế bào gốc đa năng cảm ứng là vô cùng cần thiết. Các chiến lược mới nổi được kỳ vọng sẽ cung cấp khả năng đưa ra các biến đổi di truyền đa dạng hiệu quả hơn, từ những sửa đổi nhỏ như sửa đổi một nucleotide đến khuếch đại hoặc xóa toàn bộ gen, tất cả đều có mức độ đặc hiệu mục tiêu cao. Cho đến nay, một số kỹ thuật đã được áp dụng trong các nghiên cứu tế bào gốc để chỉnh sửa trực tiếp bộ gen tế bào (ZFN, TALEN hoặc CRISPR/Cas9). Trong bài đánh giá này, chúng tôi tóm tắt các chiến lược đưa gen cụ thể đã được áp dụng cho các nghiên cứu tế bào gốc cùng với các kỹ thuật chỉnh sửa bộ gen, cho phép sửa đổi trực tiếp các trình tự DNA nội sinh trong bối cảnh nghiên cứu ung thư."} +{"idx": 1900043, "en": "Background Low levels of estrogen can cause osteoporosis and usually occur during a woman's menopausal phase. Osteoporosis can lead to bone resorption, the absence of osseointegration, and implant failure. The aim of this study is to determine the expression of transforming growth factor-beta 1 (TGF-β1), runt-related transcription factor (RUNX2), and osteoblasts in mandibular rats with low levels of estrogen. Materials and Methods This study is an in vivo experimental research. Female Wistar rats (n = 18) were divided into two groups: (1) Postsham surgery and (2) ovariectomy group. After 12 weeks, the rats were sacrificed to identify the level of estrogen, while histological analysis was conducted to determine the level of osteoblast and the expression of TGF-β1 and RUNX2. The data were analyzed using t-test (P < 0.05). Results There were significant lower levels of estrogen and osteoblast among the ovariectomy group compared to the postsham group (P < 0.05). RUNX2 levels were found to be significantly higher in the ovariectomy group than that in the postsham group (P < 0.05). However, there were no significant differences between TGF-β1 levels within the ovariectomy and postsham groups (P > 0.05). Conclusion Ovariectomy can lead to decreased osteoblastogenesis in mandibular bone by the reduced level of osteoblast and the increased expression of TGF-β1 and RUNX2.", "gemini": "Bối cảnh Nồng độ estrogen thấp có thể gây loãng xương và thường xảy ra trong giai đoạn mãn kinh của phụ nữ. Loãng xương có thể dẫn đến tiêu xương, không có sự tích hợp xương và cấy ghép thất bại. Mục đích của nghiên cứu này là xác định biểu hiện của yếu tố tăng trưởng chuyển đổi beta 1 (TGF-β1), yếu tố phiên mã liên quan đến runt (RUNX2) và tế bào tạo xương ở chuột hàm dưới có nồng độ estrogen thấp. Vật liệu và phương pháp Nghiên cứu này là nghiên cứu thực nghiệm in vivo. Chuột Wistar cái (n = 18) được chia thành hai nhóm: (1) Nhóm phẫu thuật giả và (2) Nhóm cắt bỏ buồng trứng. Sau 12 tuần, chuột được hy sinh để xác định nồng độ estrogen, trong khi phân tích mô học được tiến hành để xác định nồng độ tế bào tạo xương và biểu hiện của TGF-β1 và RUNX2. Dữ liệu được phân tích bằng t-test (P < 0,05). Kết quả Có sự khác biệt đáng kể về nồng độ estrogen và tế bào tạo xương giữa nhóm cắt bỏ buồng trứng so với nhóm giả phẫu (P < 0,05). Nồng độ RUNX2 được phát hiện cao hơn đáng kể ở nhóm cắt bỏ buồng trứng so với nhóm giả phẫu (P < 0,05). Tuy nhiên, không có sự khác biệt đáng kể giữa nồng độ TGF-β1 trong nhóm cắt bỏ buồng trứng và nhóm giả phẫu (P > 0,05). Kết luận Cắt bỏ buồng trứng có thể dẫn đến giảm sinh xương ở xương hàm dưới do giảm nồng độ tế bào tạo xương và tăng biểu hiện của TGF-β1 và RUNX2."} +{"idx": 1900044, "en": "Background The degree of tooth enamel wear is an important aspect of the clinical acceptability of all-ceramic restorations. The purpose of this study was to compare the degree of enamel wear by feldspathic porcelain and polymer-infiltrated ceramic. Materials and Methods In this in vitro study, 10 polymer-infiltrated ceramics were prepared by creating the sections of Vita Enamic® blocks (18 mm × 14 mm × 4 mm). A total of 10 porcelain cylinders were built, and feldspathic porcelain (VMK 95, Vita) was used and fired over the metal discs. A total of 20 human maxillary premolars were assigned as antagonist. Then, 10 teeth were arranged and placed oppose to porcelain samples and 10 others were placed oppose to polymer-infiltrated-ceramic specimens in the chewing simulator. The samples were photographed before and after the chewing simulation. The difference between the two photograph was measured by stereomicroscope and Motic Image plus software 2.0 three times, and then, the mean of these three times was recorded as the amount of wear. Data were analyzed using independent samples t-test and SPSS version 16. The level of significancy was 0.05. Results The mean wear rate teeth oppose to the feldspathic porcelain group (377.294 μ) was significantly higher than that of the polymer-infiltrated ceramic group (101.755 μ) (P = 0.002). Conclusion In the present study, the amount of enamel wear of the natural teeth opposed to polymer-infiltrated ceramic was significantly lower than feldspathic porcelain.", "gemini": "Bối cảnh Mức độ mòn men răng là một khía cạnh quan trọng trong việc chấp nhận lâm sàng của phục hình toàn sứ. Mục đích của nghiên cứu này là so sánh mức độ mòn men của sứ Feldspathic và sứ thẩm thấu polymer. Vật liệu và phương pháp Trong nghiên cứu in vitro này, 10 gốm thẩm thấu polymer đã được chế tạo bằng cách tạo các phần của khối Vita Enamic® (18 mm × 14 mm × 4 mm). Tổng cộng 10 trụ sứ đã được chế tạo, và sứ Feldspathic (VMK 95, Vita) được sử dụng và nung trên đĩa kim loại. Tổng cộng 20 răng tiền hàm trên của người được chỉ định là đối kháng. Sau đó, 10 răng được sắp xếp và đặt đối diện với các mẫu sứ và 10 răng khác được đặt đối diện với các mẫu gốm thẩm thấu polymer trong máy mô phỏng nhai. Các mẫu được chụp ảnh trước và sau khi mô phỏng nhai. Sự khác biệt giữa hai bức ảnh được đo bằng kính hiển vi lập thể và phần mềm Motic Image plus 2.0 ba lần, sau đó, giá trị trung bình của ba lần này được ghi lại là lượng mòn. Dữ liệu được phân tích bằng t-test mẫu độc lập và phần mềm SPSS phiên bản 16. Mức ý nghĩa là 0,05. Kết quả Tỷ lệ mòn trung bình của răng đối diện với nhóm sứ Feldspathic (377,294 μ) cao hơn đáng kể so với nhóm gốm thẩm thấu polymer (101,755 μ) (P = 0,002). Kết luận Trong nghiên cứu hiện tại, lượng mòn men của răng tự nhiên đối diện với gốm thẩm thấu polymer thấp hơn đáng kể so với sứ Feldspathic."} +{"idx": 1900045, "en": "Background This study aims to compare the antimicrobial activity of theobromine and two commercially available kid's fluoride toothpastes. Materials and Methods In this in vitro study a chocolate-based nonfluoride toothpaste, Theobromine (Group A) and two commercially available kid's fluoride toothpaste, Kidodent (Group B), Colgate kids toothpaste (Group C) were used in this study. Freeze-dried stock culture of the strains of Streptococcus mutans, Lactobacillus acidophilus, Enterococcus faecalis, and Candida albicans was cultured in their respective selective media. Dentifrices were added to the wells punched out in the culture media and incubated. The zones of inhibition were measured to find out the antimicrobial activity. all data statistically analyzed SPSS using Kolmogorov-Smironov and Shapiro-Wilk's tests. one-way ANOVA was done and Turkey's honest significant difference post hoc test was done for pairwise comparison P ≤ 0.001. Results Theobromine showed a greater zone of inhibition, which was statistically significant when compared to other two kid's fluoride toothpastes. Conclusion Theobromine shows more antimicrobial effect against S. mutans, L. acidophilus, and E. faecalis, whereas almost a neutral effect with C. albicans. Theobromine though nonfluoridated toothpaste showed greater zones of inhibition than other commercially available fluoridated kid's toothpastes.", "gemini": "Mục tiêu Nghiên cứu này nhằm so sánh hoạt động kháng khuẩn của theobromine và hai loại kem đánh răng có chứa fluoride dành cho trẻ em được bán trên thị trường. Vật liệu và Phương pháp Trong nghiên cứu in vitro này, một loại kem đánh răng không chứa fluoride có nguồn gốc từ sô cô la, Theobromine (Nhóm A) và hai loại kem đánh răng có chứa fluoride dành cho trẻ em được bán trên thị trường, Kidodent (Nhóm B), kem đánh răng Colgate dành cho trẻ em (Nhóm C) đã được sử dụng trong nghiên cứu này. Nuôi cấy đông khô của các chủng Streptococcus mutans, Lactobacillus acidophilus, Enterococcus faecalis và Candida albicans được nuôi cấy trong môi trường chọn lọc tương ứng của chúng. Các loại kem đánh răng được thêm vào các giếng đã được đục trong môi trường nuôi cấy và ủ. Các vùng ức chế được đo để tìm ra hoạt động kháng khuẩn. Tất cả dữ liệu được phân tích thống kê bằng SPSS sử dụng các xét nghiệm Kolmogorov-Smironov và Shapiro-Wilk. Phân tích phương sai một chiều đã được thực hiện và kiểm định sự khác biệt có ý nghĩa trung thực của Turkey đã được thực hiện để so sánh từng cặp P ≤ 0,001. Kết quả Theobromine cho thấy vùng ức chế lớn hơn, có ý nghĩa thống kê khi so sánh với hai loại kem đánh răng có chứa fluoride dành cho trẻ em khác. Kết luận Theobromine cho thấy hiệu quả kháng khuẩn cao hơn đối với S. mutans, L. acidophilus và E. faecalis, trong khi hiệu quả trung tính đối với C. albicans. Mặc dù không chứa fluoride, nhưng theobromine cho thấy vùng ức chế lớn hơn so với các loại kem đánh răng có chứa fluoride dành cho trẻ em khác được bán trên thị trường."} +{"idx": 1900046, "en": "Background In saliva, neutrophil constitutes the most prominent first-line defense of immune cells against pathogenic microbes. The importance of neutrophils to the host immune systems of neutropenic or patients disabled with regard to their neutrophil function results in a tendency toward serious infections, such as early childhood caries (ECC). The cytoplasmic granules present in neutrophils play a major role in neutrophil-mediated inflammation. Azurophilic granules contain antimicrobial proteins, such as defensin, a human antimicrobial peptide (HNP 1-3). The aim of this study is to analyze the correlation of HNP 1-3 secretion with CD63 expression on the surface of salivary neutrophils. Materials and Methods This study constituted a cross-sectional, analytical observational study. Saliva taken from preschoolchildren between the ages of 4-6 years who had been divided into two groups, i.e., early childhood caries group with decayed, extracted, filled teeth (def-t) index >6 and caries free with def-t = 0, was subjected to a HNP 1-3 secretion test using ELISA assay and an expression test for CD63 by means of a flow cytometry test. The results obtained were analyzed using independent t-test and Pearson correlation (P < 0.05). Results The secretion of HNP 1-3 in the saliva of ECC was higher (172.6 ± 41.64) compared to that of caries-free cases (140.39 ± 31.91), whereas the level of CD63 salivary expression in ECC was lower (2.32 ± 0.57) than in the presence of caries (2.67 ± 0.46). Conclusion In ECC cases, saliva increases HNP 1-3 secretion but decreases CD63 expression on the surface of salivary neutrophils.", "gemini": "Bối cảnh Trong nước bọt, bạch cầu trung tính chiếm phần lớn hàng phòng thủ đầu tiên của các tế bào miễn dịch chống lại vi khuẩn gây bệnh. Tầm quan trọng của bạch cầu trung tính đối với hệ thống miễn dịch của vật chủ bị giảm bạch cầu trung tính hoặc bệnh nhân bị suy giảm chức năng bạch cầu trung tính dẫn đến xu hướng mắc các bệnh nhiễm trùng nghiêm trọng, chẳng hạn như sâu răng ở trẻ nhỏ (ECC). Các hạt có trong tế bào chất của bạch cầu trung tính đóng vai trò chính trong quá trình viêm do bạch cầu trung tính. Các hạt ái toan chứa các protein kháng khuẩn, chẳng hạn như defensin, một peptit kháng khuẩn của con người (HNP 1-3). Mục đích của nghiên cứu này là phân tích mối tương quan giữa tiết HNP 1-3 với biểu hiện CD63 trên bề mặt bạch cầu trung tính trong nước bọt. Vật liệu và Phương pháp Nghiên cứu này là một nghiên cứu quan sát phân tích cắt ngang. Nước bọt lấy từ trẻ mẫu giáo từ 4-6 tuổi được chia thành hai nhóm, tức là nhóm sâu răng ở trẻ nhỏ có chỉ số răng sâu, nhổ, trám (def-t) > 6 và không sâu răng với def-t = 0, đã được thử nghiệm tiết HNP 1-3 bằng xét nghiệm ELISA và xét nghiệm biểu hiện CD63 bằng phương pháp xét nghiệm lưu lượng tế bào. Các kết quả thu được đã được phân tích bằng kiểm định t độc lập và hệ số tương quan Pearson (P < 0,05). Kết quả Tiết HNP 1-3 trong nước bọt của ECC cao hơn (172,6 ± 41,64) so với trường hợp không sâu răng (140,39 ± 31,91), trong khi mức biểu hiện CD63 trong nước bọt của ECC thấp hơn (2,32 ± 0,57) so với trường hợp có sâu răng (2,67 ± 0,46). Kết luận Trong trường hợp ECC, nước bọt làm tăng tiết HNP 1-3 nhưng làm giảm biểu hiện CD63 trên bề mặt bạch cầu trung tính trong nước bọt."} +{"idx": 1900047, "en": "Background It is cleared that some probiotic strains inhibit biofilm formation of oral bacteria, but its mechanisms are not clearly understood yet. It is proposed that one of the mechanisms can be biosurfactant production, a structurally diverse group of surface-active compounds synthesized by microorganisms. Hence, this study focused on the evaluation of the anti-biofilm and antiadhesive activities of the L. rhamnosus derived-biosurfactant against Streptococcus mutans and its effect on gtfB/C and ftf genes expression level. Materials and Methods In this in vitro study Lactobacillus rhamnosus ATCC7469 overnight culture was used for biosurfactant production. The biosurfactant effect on the surface tension reduction was confirmed by drop collapse method. Chemical bonds in the biosurfactant were identified by Fourier transform infrared (FTIR). Anti-biofilm and antiadhesive activities of the biosurfactant were determined on glass slides and in 96-well culture plates, respectively. The effect of the biosurfactant on gtfB/C and ftf genes expression level was also investigated after biofilm formation, total RNA extraction, and reverse transcription by quantitative real-time reverse transcriptase polymerase chain reaction (PCR) assay (quantitative PCR). The data were assessed by one-way analysis of variance in the Tukey-Kramer postdeviation test for all pairs. P < 0.05 was considered statistically significant. Results The FTIR results of biosurfactant showed that it was protein rich. It also showed anti-biofilm formation activity on the glass slide and antiadhesive activity till 40% on microtiter plate wells. It also showed a significant reduction (P < 0.05) in gtfB/C and ftf genes expression level. Conclusion L. rhamnosus-derived biosurfactant exhibits a significant inhibitory effect on biofilm formation ability of S. mutans due to downregulation of biofilm formation associated genes, gtfB/C and ftf. L. rhamnosus-derived biosurfactant with substantial antiadhesive activity is suitable candidates for use in new generations of microbial antiadhesive agents.", "gemini": "Bối cảnh Rõ ràng là một số chủng lợi khuẩn ức chế sự hình thành màng sinh học của vi khuẩn trong miệng, nhưng cơ chế của nó vẫn chưa được hiểu rõ. Người ta cho rằng một trong những cơ chế có thể là sản xuất chất hoạt động bề mặt sinh học, một nhóm các hợp chất hoạt động bề mặt có cấu trúc đa dạng do vi sinh vật tổng hợp. Do đó, nghiên cứu này tập trung vào việc đánh giá các hoạt động chống màng sinh học và chống dính của chất hoạt động bề mặt sinh học có nguồn gốc từ L. rhamnosus đối với Streptococcus mutans và tác động của nó đến mức biểu hiện gen gtfB/C và ftf. Vật liệu và Phương pháp Trong nghiên cứu in vitro này, nuôi cấy qua đêm Lactobacillus rhamnosus ATCC7469 đã được sử dụng để sản xuất chất hoạt động bề mặt sinh học. Tác dụng của chất hoạt động bề mặt sinh học đối với việc giảm sức căng bề mặt đã được xác nhận bằng phương pháp làm sụp đổ giọt. Các liên kết hóa học trong chất hoạt động bề mặt sinh học được xác định bằng phương pháp hồng ngoại chuyển đổi Fourier (FTIR). Các hoạt động chống màng sinh học và chống dính của chất hoạt động bề mặt sinh học đã được xác định trên các phiến kính và trong các đĩa nuôi cấy 96 giếng. Tác dụng của chất hoạt động bề mặt sinh học đối với mức biểu hiện gen gtfB/C và ftf cũng được nghiên cứu sau khi hình thành màng sinh học, chiết xuất tổng RNA và phiên mã ngược bằng xét nghiệm phản ứng chuỗi polymerase phiên mã ngược thời gian thực định lượng (PCR) (PCR định lượng). Dữ liệu được đánh giá bằng phân tích phương sai một chiều trong kiểm định độ lệch chuẩn Tukey-Kramer cho tất cả các cặp. P < 0,05 được coi là có ý nghĩa thống kê. Kết quả Kết quả FTIR của chất hoạt động bề mặt sinh học cho thấy nó rất giàu protein. Nó cũng cho thấy hoạt động hình thành màng sinh học trên phiến kính và hoạt động chống dính tới 40% trên các giếng của đĩa vi lượng. Nó cũng cho thấy sự giảm đáng kể (P < 0,05) trong mức biểu hiện gen gtfB/C và ftf. Kết luận Chất hoạt động bề mặt sinh học có nguồn gốc từ L. rhamnosus thể hiện tác dụng ức chế đáng kể đối với khả năng hình thành màng sinh học của S. mutans do sự điều chỉnh giảm các gen liên quan đến sự hình thành màng sinh học, gtfB/C và ftf. Chất hoạt động bề mặt sinh học có nguồn gốc từ L. rhamnosus với hoạt tính chống dính đáng kể là ứng cử viên phù hợp để sử dụng trong các thế hệ mới của các tác nhân chống dính của vi khuẩn."} +{"idx": 1900048, "en": "Background This experimental study assessed reverse torque of indexed and nonindexed (NI) abutments in Morse Taper (MT) implants in a mechanical fatigue test. Materials and Methods In this experimental study It was used 37 implants MT and over them installed Pilar Flex abutments (4.8 mm × 6 mm × 1.5 mm). The groups were as follows: Group A used 19 MT implants with a NI Pilar Flex abutment loaded with 32 N/cm and Group B used 18 MT implants with an indexed (IN) Pilar Flex abutment loaded with 20 N/cm. The abutments were tested according to ISO standard 14801/2007. The specimens were installed at 30° from the axial axis and underwent a 133 N load, 4 Hz frequency, and 1,000,000 cycles. Once the test was completed, the reverse torque was provided by an electronic torque meter. Data were submitted to statistical analysis using the t-test for independent samples and paired t-test. The significance level was considered P < 0.05. Results Results obtained showed that the indexed Pilar Flex abutment had a percentage of torque loss from the initial torque of 49% and the NI Pilar Flex abutment lost 14%. Paired Student's t-tests revealed that for both NI (P < 0.001) and indexed (P < 0.001) abutments, the counter torque values were significantly lower than those applied at the initial torque. Conclusion According on the methodology used, the NI Pilar Flex abutment was more effective regarding the reverse torque in single-unit implant prostheses versus the indexed Pilar Flex abutment. A greater loosening in the indexed Pilar Flex abutment retaining screw was noted in the reverse torque test, and the Pilar Flex abutment failed to show good outcomes related to the cold welding effect.", "gemini": "Bối cảnh Nghiên cứu thực nghiệm này đánh giá mô-men xoắn ngược của trụ phục hình có chỉ số và không có chỉ số (NI) trong cấy ghép Morse Taper (MT) trong thử nghiệm mỏi cơ học. Vật liệu và phương pháp Trong nghiên cứu thực nghiệm này, đã sử dụng 37 cấy ghép MT và lắp trụ phục hình Pilar Flex lên trên (4,8 mm × 6 mm × 1,5 mm). Các nhóm như sau: Nhóm A sử dụng 19 cấy ghép MT với trụ phục hình Pilar Flex NI chịu tải 32 N/cm và Nhóm B sử dụng 18 cấy ghép MT với trụ phục hình Pilar Flex có chỉ số (IN) chịu tải 20 N/cm. Các trụ phục hình được thử nghiệm theo tiêu chuẩn ISO 14801/2007. Các mẫu vật được lắp đặt ở góc 30° so với trục dọc và chịu tải 133 N, tần số 4 Hz và 1.000.000 chu kỳ. Sau khi hoàn thành thử nghiệm, mô-men xoắn ngược được cung cấp bởi máy đo mô-men xoắn điện tử. Dữ liệu được đưa vào phân tích thống kê bằng cách sử dụng kiểm định t cho các mẫu độc lập và kiểm định t ghép đôi. Mức ý nghĩa được coi là P < 0,05. Kết quả Kết quả thu được cho thấy trụ phục hình Pilar Flex có chỉ số có tỷ lệ mất mô-men xoắn so với mô-men xoắn ban đầu là 49% và trụ phục hình Pilar Flex NI mất 14%. Kiểm định t ghép đôi của Student cho thấy đối với cả trụ phục hình NI (P < 0,001) và trụ phục hình có chỉ số (P < 0,001), giá trị mô-men xoắn ngược thấp hơn đáng kể so với giá trị mô-men xoắn ban đầu. Kết luận Theo phương pháp luận được sử dụng, trụ phục hình Pilar Flex NI hiệu quả hơn về mô-men xoắn ngược trong phục hình cấy ghép đơn vị so với trụ phục hình Pilar Flex có chỉ số. Mô-men xoắn ngược cho thấy sự nới lỏng lớn hơn ở vít giữ trụ phục hình Pilar Flex có chỉ số và trụ phục hình Pilar Flex không cho thấy kết quả tốt liên quan đến hiệu ứng hàn nguội."} +{"idx": 1900049, "en": "Objective: Employed mothers face considerable amount of hazards. Especially shift work can impact pregnant women by affecting some hormones. This study was conducted to assess the adverse effects of shift work on pregnancy outcomes. Materials and methods: This historical cohort study was conducted in 2017 in order to assess the effect of shift work on pregnancy outcomes. The subjects were consecutively selected from pregnant women, which referred to Al Zahra and Shahid Beheshti hospitals, Isfahan, Iran for their pregnancy care. The effect of shift work on pregnancy and labor complications (low birth weight, small for gestational age, pre-eclampsia and eclampsia, intra-uterine growth retardation, spontaneous abortion, preterm delivery, excessive bleeding during labor, and type of labor) was assessed. The effect was adjusted for occupation and number of children as well. Data were analyzed by SPSS (ver. 17) usingT-test, chi-Square test and logistic regression analysis. Results: Totally, 429 pregnant women entered the study. There was not a statistically significant difference between morning and shift workers regarding age. It was found that shift work probably increases the incidence of small for gestational age, pre-eclampsia and eclampsia, intra-uterine growth retardation, spontaneous abortion, and preterm delivery, but after adjustment for job and number of children the effect was observed only on preterm delivery. Conclusion: Working in a rapid cycling schedule of shift work may cause an increase in the incidence of preterm delivery in pregnant mothers.", "gemini": "Mục tiêu: Những bà mẹ đi làm phải đối mặt với rất nhiều nguy cơ. Đặc biệt, làm việc theo ca có thể ảnh hưởng đến phụ nữ mang thai bằng cách tác động đến một số hormone. Nghiên cứu này được thực hiện để đánh giá tác động xấu của việc làm việc theo ca đối với kết quả mang thai. Vật liệu và phương pháp: Nghiên cứu đoàn hệ hồi cứu này được tiến hành vào năm 2017 nhằm đánh giá tác động của việc làm việc theo ca đối với kết quả mang thai. Các đối tượng được chọn liên tiếp từ những phụ nữ mang thai, những người đã đến các bệnh viện Al Zahra và Shahid Beheshti, Isfahan, Iran để chăm sóc thai sản. Tác động của việc làm việc theo ca đối với các biến chứng trong thai kỳ và chuyển dạ (trẻ nhẹ cân, nhỏ so với tuổi thai, tiền sản giật và sản giật, chậm phát triển trong tử cung, sảy thai tự nhiên, sinh non, chảy máu quá nhiều khi chuyển dạ và loại chuyển dạ) đã được đánh giá. Tác động cũng được điều chỉnh theo nghề nghiệp và số con. Dữ liệu được phân tích bằng SPSS (phiên bản 17) sử dụng kiểm định T, kiểm định chi bình phương và phân tích hồi quy logistic. Kết quả: Tổng cộng có 429 phụ nữ mang thai tham gia nghiên cứu. Không có sự khác biệt có ý nghĩa thống kê giữa những người làm việc buổi sáng và làm việc theo ca về tuổi tác. Người ta thấy rằng làm việc theo ca có thể làm tăng tỷ lệ trẻ nhỏ so với tuổi thai, tiền sản giật và sản giật, chậm phát triển trong tử cung, sảy thai tự nhiên và sinh non, nhưng sau khi điều chỉnh theo nghề nghiệp và số con thì tác động chỉ được quan sát thấy ở sinh non. Kết luận: Làm việc theo ca luân phiên nhanh có thể làm tăng tỷ lệ sinh non ở bà mẹ mang thai."} +{"idx": 1900050, "en": "Objective: Gestational diabetes mellitus is the most common metabolic and endocrine perinatal complication and is a growing health problem worldwide. Considering the fetal programming and its contribution as one of the evolutionary origins of human diseases, it is very important to improve the glucose metabolism in pregnant women, determination of other nutrients, preventing excessive accumulation of fetal fats, emphasis on weight loss measures before pregnancy, dietary intake with low-fat healthy food and prevention of abundant weight loss. In this paper, we have provided a brief review on dietary intake and dietary interventions in GDM from the perspective of nutrition science attending the physiopathology and etiology of the disease. Materials and methods: Electronic search for English and Persian articles has been perform in databases, including Google Scholar, PubMed ,Scopus, Cochrane central ,Science direct, ISC, SID, Magiran, Iran Medex, and Med Libby key words: gestational diabetes, gestational diabetes mellitus, nutrition, macronutrient, micronutrient, Diabetes. All available articles (cross-sectional, descriptive-analytic, and clinical studies with desirable design and review quality studies were used. Reference books including Krause's Food and the Nutrition Care, The Williams Obstetrics editions of the 14th (2017) and the 24th edition (2014) were also reviewed. Results: Nutrition therapy and physical activity are the initial treatment of GDM. Proper and flexible methods of nutrition therapy that successfully regulate maternal glycaemia while improving expected fetal growth have extensive concepts. Meanwhile, dietary supplements with proven beneficial effects can play an important role in improving deficiencies and improving the metabolic profile of patients. Conclusion: Nutritional management is the main treatment for gestational diabetes mellitus and overweight/obesity is the principal contest in patient counseling and interventions during pregnancy. Despite extensive researches carried out, this field is an active research area and requires more clinical research to minimize maternal and fetal complications.", "gemini": "Mục tiêu: Đái tháo đường thai kỳ là biến chứng chu sinh về chuyển hóa và nội tiết phổ biến nhất và là vấn đề sức khỏe ngày càng gia tăng trên toàn thế giới. Xem xét quá trình lập trình của thai nhi và sự đóng góp của nó như một trong những nguồn gốc tiến hóa của bệnh ở người, việc cải thiện chuyển hóa glucose ở phụ nữ mang thai, xác định các chất dinh dưỡng khác, ngăn ngừa tích tụ quá nhiều mỡ ở thai nhi, nhấn mạnh vào các biện pháp giảm cân trước khi mang thai, chế độ ăn uống với thực phẩm lành mạnh ít chất béo và ngăn ngừa mất cân quá nhiều là rất quan trọng. Trong bài báo này, chúng tôi đã cung cấp một đánh giá tóm tắt về chế độ ăn uống và các biện pháp can thiệp chế độ ăn uống trong đái tháo đường thai kỳ theo quan điểm của khoa học dinh dưỡng tham gia vào sinh lý bệnh và nguyên nhân của bệnh. Vật liệu và phương pháp: Tìm kiếm điện tử các bài báo tiếng Anh và tiếng Ba Tư đã được thực hiện trong các cơ sở dữ liệu, bao gồm Google Scholar, PubMed, Scopus, Cochrane Central, Science Direct, ISC, SID, Magiran, Iran Medex và Med Libby. Các từ khóa: đái tháo đường thai kỳ, đái tháo đường thai kỳ, dinh dưỡng, đa lượng dinh dưỡng, vi chất dinh dưỡng, đái tháo đường. Tất cả các bài báo có sẵn (nghiên cứu cắt ngang, phân tích mô tả và nghiên cứu lâm sàng có thiết kế mong muốn và nghiên cứu chất lượng đánh giá) đã được sử dụng. Các sách tham khảo bao gồm Krause's Food and the Nutrition Care, The Williams Obstetrics, ấn bản lần thứ 14 (2017) và ấn bản lần thứ 24 (2014) cũng đã được xem xét. Kết quả: Liệu pháp dinh dưỡng và hoạt động thể chất là phương pháp điều trị ban đầu cho đái tháo đường thai kỳ. Các phương pháp liệu pháp dinh dưỡng thích hợp và linh hoạt giúp điều chỉnh thành công lượng đường huyết ở bà mẹ trong khi cải thiện sự phát triển dự kiến của thai nhi có các khái niệm mở rộng. Trong khi đó, các chất bổ sung dinh dưỡng có tác dụng có lợi đã được chứng minh có thể đóng vai trò quan trọng trong việc cải thiện tình trạng thiếu hụt và cải thiện hồ sơ chuyển hóa của bệnh nhân. Kết luận: Quản lý dinh dưỡng là phương pháp điều trị chính cho đái tháo đường thai kỳ và thừa cân/béo phì là cuộc thi chính trong tư vấn và can thiệp cho bệnh nhân trong thai kỳ. Mặc dù đã tiến hành nhiều nghiên cứu mở rộng, nhưng lĩnh vực này vẫn là một lĩnh vực nghiên cứu tích cực và cần nhiều nghiên cứu lâm sàng hơn để giảm thiểu các biến chứng cho bà mẹ và thai nhi."} +{"idx": 1900051, "en": "Objective: Over the past years, the prevalence and the progression rate of HIV infection in Iran especially through high-risk sexual relationships have regrettably been reported at very high levels. This cross-sectional study tries to analyze stigma, mental health, and coping skills on risky behaviors in HIV-positive adults in Tehran- Iran. Materials and methods: This cross-sectional study was conducted on a sample of 450 HIV-positive adults. Participants completed a socio-demographic questionnaire, the General HealthQuestionnaire-28, the Berger HIV Stigma Scale as well as the Lazarus Ways of Coping Questionnaire (WOCQ). To analyze the data, the independent-samples t-test and Pearson Correlation were used. Results: The findings of this study revealed that mental health, stigma, and avoidance-escape coping mechanisms were correlated with risky behaviors (p ˂ 0.05).Furthermore, the amount of stigma among female individuals compared to men was reported at higher levels and mental health status in the given group was lower than among male individuals. Conclusion: It seems that psychological treatment techniques could be effective in improving mental health and reducing risky behaviors.", "gemini": "Mục tiêu: Trong những năm qua, tỷ lệ mắc và tỷ lệ tiến triển của nhiễm HIV ở Iran, đặc biệt là thông qua các mối quan hệ tình dục có nguy cơ cao, đáng tiếc là đã được báo cáo ở mức rất cao. Nghiên cứu cắt ngang này cố gắng phân tích sự kỳ thị, sức khỏe tâm thần và các kỹ năng đối phó với các hành vi có nguy cơ ở những người lớn nhiễm HIV tại Tehran-Iran. Vật liệu và phương pháp: Nghiên cứu cắt ngang này được tiến hành trên một mẫu 450 người lớn nhiễm HIV. Những người tham gia đã hoàn thành một bảng câu hỏi về nhân khẩu học xã hội, Tổng bảng câu hỏi sức khỏe-28, Thang đo kỳ thị HIV của Berger cũng như Bản câu hỏi về cách đối phó của Lazarus (WOCQ). Để phân tích dữ liệu, đã sử dụng bài kiểm tra t mẫu độc lập và Hệ số tương quan Pearson. Kết quả: Những phát hiện của nghiên cứu này cho thấy sức khỏe tâm thần, sự kỳ thị và các cơ chế đối phó trốn tránh có liên quan đến các hành vi có nguy cơ (p ˂ 0,05). Hơn nữa, mức độ kỳ thị ở những cá nhân nữ so với nam được báo cáo ở mức cao hơn và tình trạng sức khỏe tâm thần ở nhóm đã cho thấp hơn so với những cá nhân nam. Kết luận: Có vẻ như các kỹ thuật điều trị tâm lý có thể có hiệu quả trong việc cải thiện sức khỏe tâm thần và giảm các hành vi có nguy cơ."} +{"idx": 1900052, "en": "Objective: To assess and compare the normal ranges of androgen hormones level, total testosterone (TT), free testosterone (FT), dehydrotestosterone (DHT), androstenedione (A4), dehydroepiandrosterone (DHEA), and dehydroepiandrosterone sulfate (DHEAS), in Iranian women based on different statistical methods. Materials and methods: This study was conducted on previous data collected in Iranian PCOS Prevalence Study, which details have been published before. A total of 1772 women of 18-45 years were recruited from urban areas of five randomly selected provinces in different geographic regions of Iran. The natural range of androgen hormones was determined and compared by two statistical methods including k-means cluster analysis, and receiver operating characteristic curve. Results: In women younger than 35 years old with any BMI, cut-off points obtained for FAI hormone were in lower percentiles; however, in older women, the results of the two methods were almost the same. Cut-off points of DHEAS in under 35 years old women of normal and obese weight and women older than 35 years old with normal weight calculated by ROC curve method was in higher percentiles than that in the cluster analysis method. In >35 years obese women, obtained cut-off points for DHEAS ROC curve was in lower percentiles in comparison to cluster analysis Conclusion: Although our study depicts the differences among the cutoff values among two statistical methods; however, lacking a gold standard test to define hyperandrogenism, we need further studies to obtain more comprehensive results.", "gemini": "Mục tiêu: Đánh giá và so sánh các phạm vi bình thường của mức hormone androgen, tổng testosterone (TT), testosterone tự do (FT), dehydrotestosterone (DHT), androstenedione (A4), dehydroepiandrosterone (DHEA) và dehydroepiandrosterone sulfate (DHEAS), ở phụ nữ Iran dựa trên các phương pháp thống kê khác nhau. Vật liệu và phương pháp: Nghiên cứu này được tiến hành trên dữ liệu trước đó thu thập được trong Nghiên cứu về tỷ lệ mắc PCOS của Iran, thông tin chi tiết đã được công bố trước đó. Tổng cộng 1772 phụ nữ từ 18-45 tuổi được tuyển dụng từ các khu vực đô thị của năm tỉnh được lựa chọn ngẫu nhiên ở các vùng địa lý khác nhau của Iran. Phạm vi tự nhiên của hormone androgen được xác định và so sánh bằng hai phương pháp thống kê bao gồm phân tích cụm k-means và đường cong đặc tính hoạt động của máy thu. Kết quả: Ở những phụ nữ dưới 35 tuổi với bất kỳ BMI nào, các điểm cắt thu được cho hormone FAI đều nằm ở các phần trăm thấp hơn; tuy nhiên, ở những phụ nữ lớn tuổi hơn, kết quả của hai phương pháp gần như giống nhau. Điểm cắt của DHEAS ở phụ nữ dưới 35 tuổi có cân nặng bình thường và béo phì và phụ nữ trên 35 tuổi có cân nặng bình thường được tính bằng phương pháp đường cong ROC nằm ở các phần trăm cao hơn so với phương pháp phân tích cụm. Ở phụ nữ béo phì> 35 tuổi, các điểm cắt thu được cho đường cong ROC DHEAS nằm ở các phần trăm thấp hơn so với phân tích cụm. Kết luận: Mặc dù nghiên cứu của chúng tôi mô tả sự khác biệt giữa các giá trị cắt trong số hai phương pháp thống kê; tuy nhiên, thiếu một xét nghiệm chuẩn vàng để xác định tình trạng tăng androgen máu, chúng ta cần các nghiên cứu sâu hơn để có được kết quả toàn diện hơn."} +{"idx": 1900053, "en": "Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. Central MEC (CMEC) is a rare tumor which affects women more than men and is more common in the mandible. Most cases are histologically classified as low-grade tumor and radiographically appear as well-defined unilocular or multilocular radiolucent lesion, although this tumor causes destruction and metastasis to other organs. In this article, we present a rare case of CMEC in a 47-year-old woman with unilocular radiolucent lesion of the mandible. Early and accurate diagnostic approach in all lesions with clinical/radiographical bland appearance is important, and all possibilities such as a malignant lesion should be considered.", "gemini": "Ung thư biểu mô nhầy biểu bì (MEC) là bệnh ác tính tuyến nước bọt phổ biến nhất. MEC trung ương (CMEC) là một khối u hiếm gặp ảnh hưởng đến phụ nữ nhiều hơn nam giới và phổ biến hơn ở xương hàm dưới. Hầu hết các trường hợp được phân loại về mặt mô học là khối u cấp độ thấp và trên phim X-quang xuất hiện là tổn thương giảm mật độ một hoặc nhiều ổ được xác định rõ, mặc dù khối u này gây ra sự phá hủy và di căn đến các cơ quan khác. Trong bài viết này, chúng tôi trình bày một trường hợp hiếm gặp của CMEC ở một phụ nữ 47 tuổi có tổn thương giảm mật độ một ổ ở xương hàm dưới. Phương pháp tiếp cận chẩn đoán sớm và chính xác ở tất cả các tổn thương có biểu hiện lâm sàng/chụp X-quang không rõ ràng là rất quan trọng và tất cả các khả năng như tổn thương ác tính đều phải được xem xét."} +{"idx": 1900054, "en": "Objective: To study the effects of the Individual and Group Psycho-educational counseling in pregnant women on knowledge, attitude and mode of delivery. Materials and methods: This is a randomized controlled trial that carried out on 100 healthy nulliparous pregnant women with uncomplicated pregnancies, who had no contraindication for vaginal birth, but opted for a caesarean section in Medical centers of Dezful city, in the south west of Iran. Participants were randomly assigned into individual or group psycho-educational counseling from gestational week 20 and Knowledge, attitude and mode of delivery in the Individual and Group Psycho-educational counseling methods were measured. Results: All the participants (100%) in the individual and a majority (92%) in the group counseling changed their preference for birth method to vaginal birth after the counseling intervention. Baseline mean scores of knowledge and attitude into birth method selection were equal between groups. After the counseling intervention the mean scores increased significantly for knowledge in both the individual and group counseling groups: 12.96 and 12.88 before the intervention to 24.16 and 22.62, respectively (p < 0.001). Likewise attitude mean scores increased in both groups: 116.06 and 123.42, respectively, before the counseling sessions, that changed to 170.12 and 160.36 after the counseling sessions (p < 0.001). The differences in knowledge and attitude mean scores were statistically non-significant between groups after the intervention. Conclusion: The individual as well as the group psycho-educational counseling sessions increased the knowledge and attitude of pregnant women in relation to vaginal birth without significant differences between groups. Both methods can be recommended. A group counseling method is more effective for advising on the choice of delivery method when many women request a caesarean section without medical indications.", "gemini": "Mục tiêu: Nghiên cứu tác động của tư vấn giáo dục tâm lý cá nhân và theo nhóm đối với phụ nữ mang thai về kiến thức, thái độ và phương pháp sinh nở. Vật liệu và phương pháp: Đây là một thử nghiệm có đối chứng ngẫu nhiên được thực hiện trên 100 phụ nữ mang thai khỏe mạnh chưa sinh con với thai kỳ không biến chứng, không có chống chỉ định sinh thường nhưng đã chọn sinh mổ tại các Trung tâm y tế thành phố Dezful, phía tây nam Iran. Những người tham gia được phân ngẫu nhiên vào nhóm tư vấn giáo dục tâm lý cá nhân hoặc theo nhóm từ tuần thai thứ 20 và Kiến thức, thái độ và phương pháp sinh nở trong các phương pháp tư vấn giáo dục tâm lý cá nhân và theo nhóm đã được đo lường. Kết quả: Tất cả những người tham gia (100%) trong nhóm cá nhân và phần lớn (92%) trong nhóm tư vấn theo nhóm đã thay đổi sở thích phương pháp sinh nở sang sinh thường sau khi can thiệp tư vấn. Điểm trung bình ban đầu về kiến thức và thái độ trong lựa chọn phương pháp sinh nở là bằng nhau giữa các nhóm. Sau khi can thiệp tư vấn, điểm trung bình tăng đáng kể về kiến thức ở cả nhóm tư vấn cá nhân và nhóm: lần lượt là 12,96 và 12,88 trước can thiệp thành 24,16 và 22,62 (p < 0,001). Tương tự, điểm trung bình về thái độ cũng tăng ở cả hai nhóm: lần lượt là 116,06 và 123,42 trước các buổi tư vấn, sau các buổi tư vấn đã thay đổi thành 170,12 và 160,36 (p < 0,001). Sự khác biệt về điểm trung bình về kiến thức và thái độ không có ý nghĩa thống kê giữa các nhóm sau can thiệp. Kết luận: Các buổi tư vấn giáo dục tâm lý cá nhân cũng như theo nhóm làm tăng kiến thức và thái độ của phụ nữ mang thai liên quan đến sinh thường mà không có sự khác biệt đáng kể giữa các nhóm. Cả hai phương pháp đều có thể được khuyến nghị. Phương pháp tư vấn theo nhóm hiệu quả hơn trong việc tư vấn về lựa chọn phương pháp sinh nở khi nhiều phụ nữ yêu cầu sinh mổ mà không có chỉ định y khoa."} +{"idx": 1900055, "en": "Background Stevioside is a natural herbal sweetener extracted from Stevia rebaudiana. An in vitro study has proved the antibacterial efficacy of 0.2% Stevia aqueous solution against Streptococcus mutans and Lactobacillus acidophilus, and the present study was conducted to clinically evaluate the efficacy of Stevia leaf extract and Stevia product on plaque pH, when compared with sucrose solution. Materials and Methods A clinical trial was conducted among a sample of 22 undergraduate students who volunteered. After obtaining consent, students were instructed not to brush at night and not to use any mouth rinse during the course of the study. Baseline plaque pH was measured in situ using digital pH meter. Students were asked to rinse for 1 min with 0.2% aqueous solution of Stevia leaf extract and plaque pH was measured in situ at 4 time points (5, 10, 15, and 30 min) after each rinse. After a washout period of 2 days, 10% sucrose and 1% Stevia product solutions were similarly tested. Statistical analysis was performed using analysis of variance (ANOVA) test and repeated measures ANOVA. Tukey's HSD test was used to obtain multiple comparisons. The level of significance was set to be at P < 0.05. Results At 5, 10, 15, and 30 min, a significant difference in mean plaque pH values was observed between three test solutions (P < 0.000). Post hoc Tukey's HSD test showed that the difference in mean pH values between aqueous Stevia extract and sucrose and Stevia product and sucrose was highly significant (P < 0.000). Conclusion Stevia leaf extract and commercially available Stevia product did not significantly affect plaque pH values, implying that two solutions are non-fermentable and do not support bacterial survival.", "gemini": "Bối cảnh Stevioside là một chất tạo ngọt tự nhiên có nguồn gốc từ cây cỏ ngọt Stevia rebaudiana. Một nghiên cứu trong ống nghiệm đã chứng minh hiệu quả kháng khuẩn của dung dịch nước Stevia 0,2% đối với Streptococcus mutans và Lactobacillus acidophilus, và nghiên cứu hiện tại được tiến hành để đánh giá hiệu quả lâm sàng của chiết xuất lá Stevia và sản phẩm Stevia đối với độ pH mảng bám, khi so sánh với dung dịch sucrose. Vật liệu và phương pháp Một thử nghiệm lâm sàng đã được tiến hành trên một mẫu gồm 22 sinh viên đại học tình nguyện. Sau khi có sự đồng ý, sinh viên được hướng dẫn không đánh răng vào ban đêm và không sử dụng bất kỳ loại nước súc miệng nào trong suốt quá trình nghiên cứu. Độ pH mảng bám ban đầu được đo tại chỗ bằng máy đo pH kỹ thuật số. Sinh viên được yêu cầu súc miệng trong 1 phút bằng dung dịch nước chiết xuất lá Stevia 0,2% và độ pH mảng bám được đo tại chỗ tại 4 thời điểm (5, 10, 15 và 30 phút) sau mỗi lần súc miệng. Sau thời gian rửa trôi là 2 ngày, dung dịch sucrose 10% và dung dịch sản phẩm Stevia 1% cũng được thử nghiệm tương tự. Phân tích thống kê được thực hiện bằng cách sử dụng phép thử phân tích phương sai (ANOVA) và phép thử ANOVA lặp lại. Phép thử HSD của Tukey được sử dụng để so sánh nhiều lần. Mức ý nghĩa được đặt ở mức P < 0,05. Kết quả Vào phút thứ 5, 10, 15 và 30, có sự khác biệt đáng kể về giá trị độ pH mảng bám trung bình giữa ba dung dịch thử (P < 0,000). Phép thử HSD của Tukey sau thử nghiệm cho thấy sự khác biệt về giá trị độ pH trung bình giữa chiết xuất Stevia trong nước và sucrose và sản phẩm Stevia và sucrose là rất đáng kể (P < 0,000). Kết luận Chiết xuất lá Stevia và sản phẩm Stevia có sẵn trên thị trường không ảnh hưởng đáng kể đến giá trị độ pH mảng bám, ngụ ý rằng hai dung dịch này không lên men được và không hỗ trợ sự sống của vi khuẩn."} +{"idx": 1900056, "en": "Objective: To evaluate presence of different subtypes and genetic variations of JC virus in different geographical areas is a useful tool for reconstructing of the genetic information and understanding of the evolution of the virus and also in tracing of the last and present history of human immigration. Materials and methods: This study aimed to investigate the reactivation of different genotypes of JC virus in kidney and its excretion in the urine of the 50 pregnant and 50 non-pregnant women. Phenol-chloroform method was used to extract DNA. Oligo 7 and MEGA 7 software were used for designing nested PCR specific primers based on vp1 capsid gene, and construction of phylogenetic tree, respectively. Fisher's exact test was used for statistical analyses. Results: All of the positive samples were sequenced and according to them, genotypes 1 and 3 of the virus were detected for the first time in pregnant and non-pregnant women in Asia. The frequency of genotypes 1 and 3 were 14.28% and 85.71% respectively. Conclusion: For the first time genotype 3 was reported as the frequent genotype in pregnant women in Asia. Confirming these needs more studies particularly with a higher number of cases and full genome sequencing of isolated JCVs.", "gemini": "Mục tiêu: Đánh giá sự hiện diện của các phân nhóm khác nhau và các biến thể di truyền của vi-rút JC ở các khu vực địa lý khác nhau là một công cụ hữu ích để tái tạo thông tin di truyền và hiểu biết về quá trình tiến hóa của vi-rút và cũng để theo dõi lịch sử di cư của con người trong quá khứ và hiện tại. Vật liệu và phương pháp: Nghiên cứu này nhằm mục đích điều tra sự tái hoạt của các kiểu gen khác nhau của vi-rút JC ở thận và sự bài tiết của nó trong nước tiểu của 50 phụ nữ mang thai và 50 phụ nữ không mang thai. Phương pháp phenol-chloroform được sử dụng để chiết xuất DNA. Phần mềm Oligo 7 và MEGA 7 được sử dụng để thiết kế các đoạn mồi PCR lồng nhau đặc hiệu dựa trên gen capsid vp1 và xây dựng cây phát sinh loài tương ứng. Thử nghiệm chính xác của Fisher được sử dụng để phân tích thống kê. Kết quả: Tất cả các mẫu dương tính đều được giải trình tự và theo đó, kiểu gen 1 và 3 của vi-rút được phát hiện lần đầu tiên ở phụ nữ mang thai và không mang thai ở châu Á. Tần số của kiểu gen 1 và 3 lần lượt là 14,28% và 85,71%. Kết luận: Lần đầu tiên kiểu gen 3 được báo cáo là kiểu gen phổ biến ở phụ nữ mang thai ở châu Á. Việc xác nhận những điều này cần nhiều nghiên cứu hơn, đặc biệt là với số lượng trường hợp cao hơn và giải trình tự toàn bộ bộ gen của các JCV được phân lập."} +{"idx": 1900057, "en": "Background Frequency analysis of metastasis in patients with oral squamous cell carcinoma (OSCC) helps to partly anticipate the upcoming chance of metastasis in the patients following primary diagnosis. This study was conducted to determine the evaluation of metastasis and 5-year survival in OSCC. Materials and Methods This was a retrospective, descriptive study. A total of 96 OSCC patients were studied. Demographic data and clinical view of lesion as well as histopathological grade of patients were recorded in previously prepared forms of pathology centers. These findings were then evaluated in relation to the 5-year survival. The obtained data were fed into SPSS and analyzed by Chi-square test, t-test, one-way ANOVA, and Scheffé test. A significant level of α = 0.05 was used. Results The 5-year survival of patients was 41.7%. From among the patients, 44% were found to have local recurrence and 36% were reported to have distant metastasis. There was no significant association between the 5-year survival rate and primary grade (P = 0.105). However, it revealed a significant relationship between the 5-year survival rate and distant metastasis (P = 0. 001). Conclusion This study showed that the 5-year survival is dependent on the primary site of involvement, local recurrence, regional and distant metastasis, and mean age. However, gender (P = 0.89), grade (P = 0.105), and stage (P = 0.242) have no effect on the 5-year survival of patients.", "gemini": "Tiền cảnh Phân tích tần số di căn ở những bệnh nhân ung thư biểu mô tế bào vảy miệng (OSCC) giúp dự đoán một phần cơ hội di căn sắp tới ở những bệnh nhân sau khi chẩn đoán ban đầu. Nghiên cứu này được tiến hành để xác định đánh giá di căn và tỷ lệ sống sót sau 5 năm ở OSCC. Vật liệu và phương pháp Đây là một nghiên cứu mô tả hồi cứu. Tổng cộng có 96 bệnh nhân OSCC được nghiên cứu. Dữ liệu nhân khẩu học và quan điểm lâm sàng về tổn thương cũng như mức độ mô bệnh học của bệnh nhân được ghi lại trong các biểu mẫu đã chuẩn bị trước của các trung tâm bệnh học. Những phát hiện này sau đó được đánh giá liên quan đến tỷ lệ sống sót sau 5 năm. Dữ liệu thu được được đưa vào SPSS và phân tích bằng kiểm định Chi-square, kiểm định t, ANOVA một chiều và kiểm định Scheffé. Một mức độ đáng kể α = 0,05 đã được sử dụng. Kết quả Tỷ lệ sống sót sau 5 năm của bệnh nhân là 41,7%. Trong số những bệnh nhân này, 44% được phát hiện có tái phát tại chỗ và 36% được báo cáo có di căn xa. Không có mối liên hệ đáng kể nào giữa tỷ lệ sống sót sau 5 năm và mức độ nguyên phát (P = 0,105). Tuy nhiên, nó cho thấy mối quan hệ đáng kể giữa tỷ lệ sống sót sau 5 năm và di căn xa (P = 0,001). Kết luận Nghiên cứu này cho thấy tỷ lệ sống sót sau 5 năm phụ thuộc vào vị trí chính bị ảnh hưởng, tái phát tại chỗ, di căn khu vực và xa và tuổi trung bình. Tuy nhiên, giới tính (P = 0,89), mức độ (P = 0,105) và giai đoạn (P = 0,242) không ảnh hưởng đến tỷ lệ sống sót sau 5 năm của bệnh nhân."} +{"idx": 1900058, "en": "A tail-gut cyst can be often a misleading clinical entity. In half of the patients there is no presenting symptom. On the other half, the patients most commonly present with a variety of symptoms such as rectal pain, constipation, lower back pain, dysuria or dyspareunia. The recommended treatment of choice for the tail-gut cyst is complete surgical excision without rupture of the cyst. We present the case of a 29-year-old female with history of dyspareunia over a 5-month period, who discovered an \"ovarian\" cyst during an annual scheduled ultrasound appointment. However, the intraoperative findings were surprising. The bottomline is always to keep in mind the Pandora's Box of the retrorectal space.", "gemini": "U nang đuôi cùng thường có thể là một thực thể lâm sàng gây hiểu lầm. Ở một nửa số bệnh nhân không có triệu chứng nào xuất hiện. Ở nửa còn lại, các bệnh nhân thường biểu hiện với nhiều triệu chứng khác nhau như đau trực tràng, táo bón, đau lưng dưới, tiểu khó hoặc giao hợp đau. Phương pháp điều trị được khuyến cáo để lựa chọn cho u nang đuôi cùng là cắt bỏ hoàn toàn bằng phẫu thuật mà không làm vỡ u nang. Chúng tôi trình bày trường hợp của một phụ nữ 29 tuổi có tiền sử giao hợp đau trong hơn 5 tháng, người đã phát hiện ra một nang \"buồng trứng\" trong một cuộc hẹn siêu âm theo lịch trình hàng năm. Tuy nhiên, các phát hiện trong khi phẫu thuật lại rất đáng ngạc nhiên. Điểm mấu chốt luôn là ghi nhớ Hộp Pandora của không gian sau trực tràng."} +{"idx": 1900059, "en": "Background The diabetic subjects would have impaired oral stereognostic ability (OSA) compared with normal subjects due to diabetic neuropathy and microcirculatory disturbances. This study was conducted to compare the OSA between diabetic and nondiabetic complete denture wearers with and without denture. Materials and Methods In this in vivo study the present comparative study comprised of seventy edentulous subjects (36 males and 34 females), aged from 35 to 84 rehabilitated with complete dentures (among them 35 were diabetic and 35 subjects were nondiabetic complete denture wearer). The OSA tests were conducted using acrylic test samples of 12 shaped forms, which were placed in patient's mouth for a given period of time for identification and scored according to three-point scale as OSA score and the identification time was also recorded. The data obtained were analyzed using Chi-square test, t-test, and Pearson's correlation coefficient (P < 0.05). Results In this study, diabetic complete denture wearers got the mean OSA score of 12.43 ± 3.93 without dentures, which was lower than nondiabetic complete denture wearer group (14.82 ± 4.44). There was a significant difference (P = 0.020*) in the identification of test pieces. Conclusion Within limitations of this study, diabetic complete denture wearers showed decreased OSA than nondiabetic subjects, particularly it was significant while not wearing dentures. Oral stereognosis may be used as one of the clinical aids in predicting patient's performance to a prosthesis. Based on their response, we can educate the patient about the prognosis.", "gemini": "Tiền cảnh Các đối tượng mắc bệnh tiểu đường sẽ bị suy giảm khả năng nhận biết hình dạng bằng miệng (OSA) so với các đối tượng bình thường do bệnh thần kinh tiểu đường và rối loạn vi tuần hoàn. Nghiên cứu này được tiến hành để so sánh OSA giữa những người đeo răng giả hoàn chỉnh bị tiểu đường và không bị tiểu đường có và không có răng giả. Vật liệu và phương pháp Trong nghiên cứu in vivo này, nghiên cứu so sánh hiện tại bao gồm bảy mươi đối tượng mất răng (36 nam và 34 nữ), tuổi từ 35 đến 84 được phục hồi bằng răng giả hoàn chỉnh (trong số đó có 35 người bị tiểu đường và 35 đối tượng là người đeo răng giả hoàn chỉnh không bị tiểu đường). Các xét nghiệm OSA được tiến hành bằng cách sử dụng các mẫu thử acrylic có 12 hình dạng, được đặt trong miệng bệnh nhân trong một khoảng thời gian nhất định để nhận dạng và chấm điểm theo thang ba điểm là điểm OSA và thời gian nhận dạng cũng được ghi lại. Dữ liệu thu được được phân tích bằng kiểm định Chi-square, kiểm định t và hệ số tương quan Pearson (P < 0,05). Kết quả Trong nghiên cứu này, những người đeo răng giả hoàn chỉnh bị tiểu đường có điểm OSA trung bình là 12,43 ± 3,93 khi không đeo răng giả, thấp hơn nhóm người đeo răng giả hoàn chỉnh không bị tiểu đường (14,82 ± 4,44). Có sự khác biệt đáng kể (P = 0,020 *) trong việc nhận dạng các mẫu thử. Kết luận Trong phạm vi hạn chế của nghiên cứu này, những người đeo răng giả hoàn chỉnh bị tiểu đường cho thấy OSA giảm hơn những đối tượng không bị tiểu đường, đặc biệt là khi không đeo răng giả. Nhận dạng hình dạng bằng miệng có thể được sử dụng như một trong những công cụ hỗ trợ lâm sàng để dự đoán hiệu suất của bệnh nhân đối với phục hình. Dựa trên phản ứng của họ, chúng ta có thể giáo dục bệnh nhân về tiên lượng bệnh."} +{"idx": 1900060, "en": "Background Transtheoretical model (TTM) is one of the most commonly used methods in behavioral change modeling. The aim of this study was to conduct a systematic review (SR) to determine research gaps with regard to this template with an emphasis on intervention for patients with chronic diseases (CDs). Materials and Methods ISI-WOS, Scopus, PubMed, SID, and Magiran databases were examined systematically and on the basis of defined criteria. Titles, abstracts, and full texts of articles retrieved were examined for the presence of defined criteria. Then finalized articles were analyzed in consensus meetings. After that, references of selected articles and full text of those meeting the criteria were also analyzed. Results We screened 103 articles, excluded 27 in abstract review and 34 in full-text review, leaving 42 articles for critical appraisal. Then the references of these 42 articles were also screened. Fifty articles were excluded on abstract review and 5 on full-text review, leaving 15 articles. The result of the analysis of 57 final articles of this SR determined that 28 articles were about aspects of TTM and 5 stages of change were the most commonly used aspect. Eight articles used TTM in intervention about CDs. A total of 21 articles examined TTM's pros and cons, most of which were about TTM's pros. Conclusions The majority of studies focused on the effectiveness of TTM on the behavioral change management. This finding supported the hypothesis that TTM can be applied in the prevention of CDs.", "gemini": "Bối cảnh Mô hình chuyển đổi lý thuyết (TTM) là một trong những phương pháp được sử dụng phổ biến nhất trong mô hình thay đổi hành vi. Mục đích của nghiên cứu này là tiến hành một đánh giá có hệ thống (SR) để xác định những khoảng trống nghiên cứu liên quan đến khuôn mẫu này với trọng tâm là can thiệp cho bệnh nhân mắc các bệnh mãn tính (CD). Vật liệu và Phương pháp Các cơ sở dữ liệu ISI-WOS, Scopus, PubMed, SID và Magiran đã được kiểm tra một cách có hệ thống và trên cơ sở các tiêu chí được xác định. Các tiêu đề, tóm tắt và toàn văn của các bài báo được trích xuất đã được kiểm tra để xem có các tiêu chí đã xác định hay không. Sau đó, các bài báo đã hoàn thiện được phân tích trong các cuộc họp đồng thuận. Sau đó, các tài liệu tham khảo của các bài báo đã chọn và toàn văn của những bài báo đáp ứng các tiêu chí cũng được phân tích. Kết quả Chúng tôi đã sàng lọc 103 bài báo, loại trừ 27 bài trong quá trình xem xét tóm tắt và 34 bài trong quá trình xem xét toàn văn, còn lại 42 bài báo để đánh giá quan trọng. Sau đó, các tài liệu tham khảo của 42 bài báo này cũng được sàng lọc. Năm mươi bài báo đã bị loại trừ trong quá trình xem xét tóm tắt và 5 bài trong quá trình xem xét toàn văn, còn lại 15 bài báo. Kết quả phân tích 57 bài báo cuối cùng của SR này xác định rằng 28 bài báo liên quan đến các khía cạnh của TTM và 5 giai đoạn thay đổi là khía cạnh được sử dụng phổ biến nhất. Tám bài báo đã sử dụng TTM trong can thiệp về CD. Tổng cộng có 21 bài báo đã xem xét ưu và nhược điểm của TTM, trong đó hầu hết là về ưu điểm của TTM. Kết luận Phần lớn các nghiên cứu tập trung vào hiệu quả của TTM trong việc quản lý thay đổi hành vi. Phát hiện này ủng hộ giả thuyết rằng TTM có thể được áp dụng để phòng ngừa các CD."} +{"idx": 1900061, "en": "Background Self-learning module (SLM) is designed for independent learning. In this study, the hypothesis that SLM is effective to some extent in improving clinical competence in nursing students was tested. Materials and Methods The study employed a randomized controlled design. In this study, 46 nursing students were randomized into the control or intervention group. Study tools included a demographic information form and nursing skills checklist. Unlike the control group, the intervention group received clinical SLM. Nursing students' clinical competency was measured at the end of the intervention through objective structured clinical examination. Statistical analysis was performed in SPSS software. A p value < 0.050 was considered statistically significant. Results In total, 46 students participated in this study. The Mean (SD) age of the study participants was 18.80 (1.06) years. There was a significant difference in students' clinical competency between the control and intervention groups (Mann-Whitney U-test: p = 0.010). Mean (SD) of clinical competency in the control and SLM groups was 58.19 (6.41) and 62.83 (6.05), respectively. Results of the group equivalency test indicated that all demographic variables were equivalent between the groups. Moreover, there was no significant difference between women and men in terms of clinical competency. Conclusions According to the results, the use of SLM could help the students enhance the quality of nursing clinical competency.", "gemini": "Bối cảnh Mô-đun tự học (SLM) được thiết kế để học tập độc lập. Trong nghiên cứu này, giả thuyết cho rằng SLM có hiệu quả ở một mức độ nào đó trong việc cải thiện năng lực lâm sàng ở sinh viên điều dưỡng đã được kiểm tra. Vật liệu và Phương pháp Nghiên cứu sử dụng thiết kế đối chứng ngẫu nhiên. Trong nghiên cứu này, 46 sinh viên điều dưỡng được phân ngẫu nhiên vào nhóm đối chứng hoặc nhóm can thiệp. Các công cụ nghiên cứu bao gồm biểu mẫu thông tin nhân khẩu học và danh sách kiểm tra các kỹ năng điều dưỡng. Không giống như nhóm đối chứng, nhóm can thiệp nhận được SLM lâm sàng. Năng lực lâm sàng của sinh viên điều dưỡng được đo lường vào cuối can thiệp thông qua kỳ thi lâm sàng có cấu trúc khách quan. Phân tích thống kê được thực hiện trong phần mềm SPSS. Giá trị p < 0,050 được coi là có ý nghĩa thống kê. Kết quả Tổng cộng có 46 sinh viên tham gia nghiên cứu này. Độ tuổi trung bình (SD) của những người tham gia nghiên cứu là 18,80 (1,06) tuổi. Có sự khác biệt đáng kể về năng lực lâm sàng của sinh viên giữa nhóm đối chứng và nhóm can thiệp (Kiểm định U-Mann-Whitney: p = 0,010). Độ tuổi trung bình (SD) về năng lực lâm sàng ở nhóm đối chứng và nhóm SLM lần lượt là 58,19 (6,41) và 62,83 (6,05). Kết quả kiểm tra tính tương đương của nhóm cho thấy tất cả các biến nhân khẩu học đều tương đương giữa các nhóm. Hơn nữa, không có sự khác biệt đáng kể giữa nam và nữ về năng lực lâm sàng. Kết luận Theo kết quả nghiên cứu, việc sử dụng SLM có thể giúp sinh viên nâng cao chất lượng năng lực lâm sàng điều dưỡng."} +{"idx": 1900062, "en": "Background Mental health problems such as anxiety and disappointment are common in patients with heart disease, resulting in poor outcomes. The purpose of this study was to compare the effectiveness of two methods of relaxation and prayer therapy on anxiety and hope in patients with coronary artery disease (CAD). Materials and Methods A quasi-experimental study was conducted in three groups of 25 subjects (i.e., two groups of relaxation and prayer therapy and one control group) and two stages (i.e., pretest and posttest) in hospitals affiliated to Isfahan University of Medical Sciences, Iran. Data collection instrument consisted of three parts: (a) demographic and individual characteristics of the subjects, (b) Beck Anxiety Inventory, and (c) Snyder's Hope Scale. The interventions consisted of Benson's relaxation and prayer therapy. Data were analyzed using descriptive and inferential statistics. Results The results showed that there was a significant difference between the mean score of anxiety after intervention in the three groups which was related to each of the relaxation and prayer therapy group with the control group. Also, the result of analysis of covariance with controlling the effect of pretest revealed significant between-subject effects of interventions on posttest scores of hope (F(2, 71) = 8.55, p = 0.012, ηp 2 = 0.12) and anxiety (F(2, 71) = 4.71, p < 0.001, ηp 2 = 0.19). Conclusions Both relaxation and prayer therapy interventions are effective in promoting hope and reducing anxiety in patients with CAD and provide optimal, short-term, and easy-to-follow options for the health team.", "gemini": "Bối cảnh Các vấn đề sức khỏe tâm thần như lo lắng và thất vọng thường gặp ở những bệnh nhân mắc bệnh tim, dẫn đến kết quả kém. Mục đích của nghiên cứu này là so sánh hiệu quả của hai phương pháp thư giãn và liệu pháp cầu nguyện đối với sự lo lắng và hy vọng ở những bệnh nhân mắc bệnh động mạch vành (CAD). Vật liệu và Phương pháp Một nghiên cứu bán thực nghiệm đã được tiến hành trên ba nhóm gồm 25 đối tượng (tức là hai nhóm thư giãn và liệu pháp cầu nguyện và một nhóm đối chứng) và hai giai đoạn (tức là tiền kiểm và hậu kiểm) tại các bệnh viện trực thuộc Đại học Khoa học Y tế Isfahan, Iran. Công cụ thu thập dữ liệu bao gồm ba phần: (a) đặc điểm cá nhân và nhân khẩu học của các đối tượng, (b) Bản kiểm kê lo âu của Beck và (c) Thang đo hy vọng của Snyder. Các can thiệp bao gồm thư giãn của Benson và liệu pháp cầu nguyện. Dữ liệu được phân tích bằng thống kê mô tả và suy luận. Kết quả Kết quả cho thấy có sự khác biệt đáng kể giữa điểm trung bình về lo lắng sau can thiệp ở ba nhóm có liên quan đến từng nhóm thư giãn và liệu pháp cầu nguyện với nhóm đối chứng. Ngoài ra, kết quả phân tích hiệp phương sai với việc kiểm soát tác dụng của tiền kiểm cho thấy có sự khác biệt đáng kể giữa các đối tượng về tác động của can thiệp đối với điểm hậu kiểm về hy vọng (F (2, 71) = 8,55, p = 0,012, ηp 2 = 0,12) và lo lắng (F (2, 71) = 4,71, p < 0,001, ηp 2 = 0,19). Kết luận Cả hai phương pháp can thiệp thư giãn và liệu pháp cầu nguyện đều có hiệu quả trong việc thúc đẩy hy vọng và giảm lo lắng ở những bệnh nhân mắc CAD và cung cấp các lựa chọn tối ưu, ngắn hạn và dễ thực hiện cho nhóm y tế."} +{"idx": 1900063, "en": "Background Breast cancer is a very common cancer in women which is diagnosable using inexpensive, accessible, and easy screening programs in the early stages of the disease. Culture, beliefs, and opinions of women affect adoption of screening techniques. Nursing professionals are supposed to be aware of such influencing factors. Therefore, this study was conducted to investigate barriers to breast self-examination (BSE) among women in Isfahan province. Materials and Methods During a cross-sectional descriptive and correlational study in 2017, 1509 women in rural and urban areas of Isfahan province were selected using multistage sampling method. Data were collected by visiting homes, using a questionnaire, and conducting interviews and were analyzed using descriptive and analytical statistics. Results The results showed that barriers consisted of lack of awareness about breast examination (p = 0.006), lack of awareness about BSE techniques (p < 0.001), and lack of training by personnel of healthcare centers (p = 0.016), which were significantly associated with residency areas (i.e., urban and rural areas). Moreover, women's educational level was significantly associated with 5 of 10 barriers and their occupation was significantly associated with 2 of 10 barriers to BSE. Conclusions Among the several factors that may affect BSE, a part of them in Isfahan women act as obstacle that they are moderated by some demographic factors such as residency areas, education level, and occupation. Therefore, nursing plans and interventions aimed at developing BSE should be tailored to suit the unique demographic characteristics of women.", "gemini": "Bối cảnh Ung thư vú là một loại ung thư rất phổ biến ở phụ nữ, có thể chẩn đoán được bằng các chương trình sàng lọc dễ tiếp cận, không tốn kém và dễ dàng trong giai đoạn đầu của bệnh. Văn hóa, niềm tin và quan điểm của phụ nữ ảnh hưởng đến việc áp dụng các kỹ thuật sàng lọc. Các chuyên gia điều dưỡng được cho là phải nhận thức được các yếu tố ảnh hưởng như vậy. Do đó, nghiên cứu này được tiến hành để điều tra những rào cản đối với việc tự kiểm tra vú (BSE) ở phụ nữ tại tỉnh Isfahan. Vật liệu và phương pháp Trong một nghiên cứu mô tả cắt ngang và tương quan vào năm 2017, 1509 phụ nữ ở các vùng nông thôn và thành thị của tỉnh Isfahan đã được chọn bằng phương pháp lấy mẫu nhiều giai đoạn. Dữ liệu được thu thập bằng cách đến thăm nhà, sử dụng bảng câu hỏi và tiến hành phỏng vấn và được phân tích bằng thống kê mô tả và phân tích. Kết quả Kết quả cho thấy các rào cản bao gồm thiếu nhận thức về khám vú (p = 0,006), thiếu nhận thức về các kỹ thuật BSE (p < 0,001) và thiếu đào tạo của nhân viên các trung tâm chăm sóc sức khỏe (p = 0,016), có liên quan đáng kể đến nơi cư trú (tức là khu vực thành thị và nông thôn). Hơn nữa, trình độ học vấn của phụ nữ có liên quan đáng kể đến 5 trong số 10 rào cản và nghề nghiệp của họ có liên quan đáng kể đến 2 trong số 10 rào cản đối với BSE. Kết luận Trong số một số yếu tố có thể ảnh hưởng đến BSE, một phần trong số đó ở phụ nữ Isfahan đóng vai trò như một trở ngại mà chúng được điều chỉnh bởi một số yếu tố nhân khẩu học như khu vực cư trú, trình độ học vấn và nghề nghiệp. Do đó, các kế hoạch và can thiệp điều dưỡng nhằm phát triển BSE nên được điều chỉnh cho phù hợp với các đặc điểm nhân khẩu học độc đáo của phụ nữ."} +{"idx": 1900064, "en": "Background Teaching methods have failed to keep up with the pace of the changing curriculum. Clinical practice, an essential part of nursing education, links theory with practice, particularly in midwifery nursing. Thus, this study aimed to compare the effects of video-assisted teaching programs and traditional demonstration on nursing students learning obstetrical palpation skills. Materials and Methods This is a quasi-experimental research work with pretest, posttest, control group design in which 60 third-year students of Bachelor of Science in Nursing were selected and assigned randomly, by lottery method, into an experimental group (video-assisted teaching program) and a control group (traditional demonstration) regarding obstetrical palpation. The data were collected through a self-designed rating scale. The validity of the rating scale was established by a panel of seven experts from the field of obstetrical and gynecological nursing, and the reliability was established through Cronbach's α(0.78), which showed the tool was consistent among the population. Results The results showed a significant difference between the pretest and posttest skill scores of students who were exposed to video-assisted teaching program and traditional demonstration (t = 18.35, p < 0.001). Although both the methods were equally effective in enhancing skill, traditional demonstration scored much better than the video-assisted teaching program when the posttest skills were compared (t = 36.40, p = 0.001). Conclusions The routine educational method, i.e., demonstration, is more effective in developing skills emphasizing the reinforcement of academicians in enhancing teaching skills by adopting blended teaching technique for enhancing memory storage, retrieval, cognition, and learning.", "gemini": "Bối cảnh Các phương pháp giảng dạy đã không theo kịp tốc độ thay đổi của chương trình giảng dạy. Thực hành lâm sàng, một phần thiết yếu của giáo dục điều dưỡng, liên kết lý thuyết với thực hành, đặc biệt là trong điều dưỡng hộ sinh. Do đó, nghiên cứu này nhằm mục đích so sánh hiệu quả của các chương trình giảng dạy có sự hỗ trợ của video và trình diễn truyền thống đối với sinh viên điều dưỡng học các kỹ năng thăm khám sản khoa. Vật liệu và phương pháp Đây là một công trình nghiên cứu bán thực nghiệm với thiết kế tiền kiểm, hậu kiểm, nhóm đối chứng trong đó 60 sinh viên năm thứ ba của Cử nhân Khoa học Điều dưỡng được chọn và phân công ngẫu nhiên, theo phương pháp xổ số, vào một nhóm thực nghiệm (chương trình giảng dạy có sự hỗ trợ của video) và một nhóm đối chứng (trình diễn truyền thống) về thăm khám sản khoa. Dữ liệu được thu thập thông qua thang đánh giá tự thiết kế. Tính hợp lệ của thang đánh giá được thiết lập bởi một nhóm bảy chuyên gia trong lĩnh vực điều dưỡng sản khoa và phụ khoa, và độ tin cậy được thiết lập thông qua Cronbach's α (0,78), cho thấy công cụ này phù hợp trong toàn dân số. Kết quả Kết quả cho thấy có sự khác biệt đáng kể giữa điểm kỹ năng tiền kiểm và hậu kiểm của sinh viên được tiếp xúc với chương trình giảng dạy có sự hỗ trợ của video và trình diễn truyền thống (t = 18,35, p < 0,001). Mặc dù cả hai phương pháp đều có hiệu quả như nhau trong việc nâng cao kỹ năng, nhưng trình diễn truyền thống đạt điểm cao hơn nhiều so với chương trình giảng dạy có sự hỗ trợ của video khi các kỹ năng hậu kiểm được so sánh (t = 36,40, p = 0,001). Kết luận Phương pháp giáo dục thường quy, tức là trình diễn, có hiệu quả hơn trong việc phát triển các kỹ năng nhấn mạnh đến việc củng cố các học giả trong việc nâng cao kỹ năng giảng dạy bằng cách áp dụng kỹ thuật giảng dạy hỗn hợp để nâng cao khả năng lưu trữ, truy xuất, nhận thức và học tập."} +{"idx": 1900065, "en": "Background There is limited research on gerontological nursing competence in hospitals. However, there is no comprehensive and integrated description of the gerontological nursing competence requirements in hospitals. The purpose of this study was to explain the components of nursing competence in caring for older people in Iranian hospitals. Materials and Methods This is a qualitative descriptive-exploratory study. The data were collected through a semi-structured interview with nurses, nurse managers, and clinical educators in teaching hospitals and nursing schools affiliated to Arak, Isfahan, and Tehran Universities of Medical Sciences in Iran from September 2015 to July 2016. Participants included 25 people who were selected by purposive and snowball sampling method. Sampling continued until data saturation. Data were analyzed using conventional content analysis method. Results Data analysis generated three main categories including (1) Patient and family centered care; (2) Process-oriented care; and (3) Self-care and continuing professional development. Conclusions Nurses should have competencies such as participation and empowerment of the patient and family; ease of comfort in the elderly; comprehensive geriatric assessment; development, implementation and evaluation of care plan; development of knowledge and clinical proficiency; and coaching so that they can work effectively during the care of the elderly. The results of this study can be used by nursing educators, nursing students, and nurses to develop their individual and professional skills in the field of gerontological nursing.", "gemini": "Bối cảnh Có rất ít nghiên cứu về năng lực điều dưỡng lão khoa tại các bệnh viện. Tuy nhiên, không có mô tả toàn diện và tích hợp về các yêu cầu năng lực điều dưỡng lão khoa tại các bệnh viện. Mục đích của nghiên cứu này là giải thích các thành phần của năng lực điều dưỡng trong việc chăm sóc người cao tuổi tại các bệnh viện Iran. Vật liệu và phương pháp Đây là một nghiên cứu mô tả-thăm dò định tính. Dữ liệu được thu thập thông qua phỏng vấn bán cấu trúc với các y tá, điều dưỡng trưởng và các nhà giáo dục lâm sàng tại các bệnh viện giảng dạy và trường điều dưỡng trực thuộc các trường Đại học Khoa học Y tế Arak, Isfahan và Tehran tại Iran từ tháng 9 năm 2015 đến tháng 7 năm 2016. Những người tham gia bao gồm 25 người được chọn theo phương pháp lấy mẫu có mục đích và theo kiểu lăn cầu tuyết. Lấy mẫu tiếp tục cho đến khi dữ liệu bão hòa. Dữ liệu được phân tích bằng phương pháp phân tích nội dung thông thường. Kết quả Phân tích dữ liệu tạo ra ba loại chính bao gồm (1) Chăm sóc tập trung vào bệnh nhân và gia đình; (2) Chăm sóc theo quy trình; và (3) Tự chăm sóc và phát triển chuyên môn liên tục. Kết luận Điều dưỡng phải có các năng lực như sự tham gia và trao quyền cho bệnh nhân và gia đình; dễ dàng thoải mái ở người cao tuổi; đánh giá toàn diện lão khoa; phát triển, thực hiện và đánh giá kế hoạch chăm sóc; phát triển kiến ​​thức và năng lực lâm sàng; và hướng dẫn để họ có thể làm việc hiệu quả trong quá trình chăm sóc người cao tuổi. Kết quả của nghiên cứu này có thể được các nhà giáo dục điều dưỡng, sinh viên điều dưỡng và điều dưỡng sử dụng để phát triển các kỹ năng cá nhân và chuyên môn của họ trong lĩnh vực điều dưỡng lão khoa."} +{"idx": 1900066, "en": "Background Attention-deficit hyperactive disorder (ADHD) is the most common behavioral disorder of childhood, and due to its chronic nature, it would affect all the aspects of life of the child and the caregiver. Therefore, this study was conducted to determine the effect of a program based on the needs of caregivers on stress, anxiety, depression, and burden of care in families of children with ADHD. Materials and Methods This randomized clinical trial was conducted on 70 family caregivers of children with ADHD from June to September of 2016. After convenience sampling, eligible samples were randomly allocated into two groups of control and intervention. The intervention group received the need-based educational program in eight sessions and the participants of the control group participated in two group sessions where they expressed their problems and experiences. Data were gathered through Zarit Burden Interview and Depression Anxiety and Stress Scale (DASS)-42 and then were analyzed using descriptive and inferential statistics. Results The mean scores of stress, anxiety, depression, and burden of care had a significant difference between both groups right after and 1 month after the intervention (p < 0.001), whereas in the control group, there was no gradual decrease in the mean scores of the dependent variables. Conclusions The need-based educational program might reduce the level of stress, anxiety, depression, and burden of care in families of children with ADHD. It is recommended to develop similar programs and evaluate them through various studies. The use of nonpharmacological treatment of these children is evident in the results.", "gemini": "Bối cảnh Rối loạn tăng động giảm chú ý (ADHD) là rối loạn hành vi phổ biến nhất ở trẻ em và do tính chất mãn tính của nó, nó sẽ ảnh hưởng đến mọi khía cạnh trong cuộc sống của trẻ và người chăm sóc. Do đó, nghiên cứu này được tiến hành để xác định hiệu quả của một chương trình dựa trên nhu cầu của người chăm sóc đối với căng thẳng, lo lắng, trầm cảm và gánh nặng chăm sóc trong các gia đình có trẻ mắc ADHD. Vật liệu và phương pháp Thử nghiệm lâm sàng ngẫu nhiên này được tiến hành trên 70 người chăm sóc gia đình của trẻ em mắc ADHD từ tháng 6 đến tháng 9 năm 2016. Sau khi lấy mẫu thuận tiện, các mẫu đủ điều kiện được phân bổ ngẫu nhiên vào hai nhóm đối chứng và can thiệp. Nhóm can thiệp nhận được chương trình giáo dục dựa trên nhu cầu trong tám buổi và những người tham gia nhóm đối chứng tham gia hai buổi thảo luận nhóm, nơi họ bày tỏ các vấn đề và kinh nghiệm của mình. Dữ liệu được thu thập thông qua Phỏng vấn gánh nặng Zarit và Thang đo căng thẳng, lo lắng và trầm cảm (DASS) -42 và sau đó được phân tích bằng số liệu thống kê mô tả và suy luận. Kết quả Điểm trung bình của căng thẳng, lo lắng, trầm cảm và gánh nặng chăm sóc có sự khác biệt đáng kể giữa hai nhóm ngay sau và 1 tháng sau khi can thiệp (p < 0,001), trong khi ở nhóm đối chứng, không có sự giảm dần nào về điểm trung bình của các biến phụ thuộc. Kết luận Chương trình giáo dục dựa trên nhu cầu có thể làm giảm mức độ căng thẳng, lo lắng, trầm cảm và gánh nặng chăm sóc trong các gia đình có trẻ mắc ADHD. Nên phát triển các chương trình tương tự và đánh giá chúng thông qua nhiều nghiên cứu khác nhau. Việc sử dụng phương pháp điều trị không dùng thuốc đối với những trẻ này đã được chứng minh trong các kết quả."} +{"idx": 1900067, "en": "Background Image-guided fine-needle aspiration has emerged as an effective diagnostic tool for precise diagnosis of deep-seated lesions. Although occasional studies have made an attempt to classify the gallbladder carcinoma on cytology, literature lacks the standardized cytological nomenclature system used for it. The present study was conducted to study the role of fine-needle aspiration cytology (FNAC) in diagnosis of gallbladder lesions with an attempt of cytomorphological classification. Methods The study included cases of image-guided FNAC of the gallbladder over a period of 3½ years. An attempt was made to categorize gallbladder lesions on basis of architectural and cytomorphological features along with analysis of management. Results The study included 433 cases and lesions were categorized on FNAC into five categories ranging from Category 1 (inadequate), Category 2 (negative for malignancy), Category 3 (atypical cells), Category 4 (highly atypical cells suggestive of malignancy), and Category 5 (positive for malignancy). The most common architectural pattern observed on FNAC of neoplasm was sheets and acini with predominance of columnar cells and adenocarcinoma being the most common malignancy. The histopathological diagnosis was available in 93 cases with cytohistopathological concordance of 94.4% in malignant cases. Conclusions Image-guided FNAC plays an important role in diagnosis of gallbladder lesions with minimal complications. The cytomorphological classification of gallbladder lesions provides an effective base for accurate diagnosis and management. Category 3 and 4 are the most ambiguous category on FNAC which should be managed by either repeat FNAC or surgery in the light of worrisome radiological features. The vigilant examination of architectural pattern and cytomorphological features of the smears may be helpful in clinching the diagnosis and precisely subtyping malignant tumors along with prognostication of these tumors.", "gemini": "Bối cảnh Chọc hút kim nhỏ có hướng dẫn hình ảnh đã nổi lên như một công cụ chẩn đoán hiệu quả để chẩn đoán chính xác các tổn thương nằm sâu. Mặc dù các nghiên cứu đôi khi đã cố gắng phân loại ung thư túi mật trên tế bào học, nhưng các tài liệu thiếu hệ thống danh pháp tế bào học chuẩn hóa được sử dụng cho nó. Nghiên cứu hiện tại được tiến hành để nghiên cứu vai trò của tế bào học chọc hút kim nhỏ (FNAC) trong chẩn đoán các tổn thương túi mật với nỗ lực phân loại hình thái tế bào. Phương pháp Nghiên cứu bao gồm các trường hợp FNAC có hướng dẫn hình ảnh của túi mật trong khoảng thời gian 3 năm rưỡi. Người ta đã cố gắng phân loại các tổn thương túi mật dựa trên các đặc điểm về kiến trúc và hình thái tế bào cùng với việc phân tích cách xử trí. Kết quả Nghiên cứu bao gồm 433 trường hợp và các tổn thương được phân loại trên FNAC thành năm loại từ Loại 1 (không đủ), Loại 2 (âm tính với ác tính), Loại 3 (tế bào bất thường), Loại 4 (tế bào rất bất thường gợi ý ác tính) và Loại 5 (dương tính với ác tính). Mẫu kiến trúc phổ biến nhất được quan sát thấy trên FNAC của khối u là các lớp và acini với sự chiếm ưu thế của các tế bào hình trụ và ung thư tuyến là loại ác tính phổ biến nhất. Chẩn đoán mô bệnh học có sẵn ở 93 trường hợp với sự nhất quán tế bào học và mô học là 94,4% ở các trường hợp ác tính. Kết luận FNAC có hướng dẫn hình ảnh đóng một vai trò quan trọng trong chẩn đoán các tổn thương túi mật với các biến chứng tối thiểu. Phân loại hình thái tế bào của các tổn thương túi mật cung cấp một cơ sở hiệu quả để chẩn đoán và xử trí chính xác. Loại 3 và 4 là loại mơ hồ nhất trên FNAC, nên được xử trí bằng cách chọc hút kim nhỏ lại hoặc phẫu thuật khi có các đặc điểm trên phim X quang đáng lo ngại. Việc kiểm tra cẩn thận mẫu kiến trúc và các đặc điểm hình thái tế bào của các vết bôi có thể hữu ích trong việc chẩn đoán và phân loại chính xác các khối u ác tính cùng với tiên lượng của các khối u này."} +{"idx": 1900068, "en": "Background The newborn individualized developmental care and assessment program (NIDCAP) is a new multifaceted approach, which is developmentally and functionally supportive of health especially in premature newborns at the neonatal intensive care unit (NICU). This study is designed for assessing the knowledge and perception of nurses as the most important members of the multidisciplinary team of NIDCAP about this program. Materials and Methods This cross-sectional study was conducted on 120 nurses working in the NICUs of Alzahra, Taleghani, and Children hospitals affiliated to the educational and treatment centers of Tabriz University of Medical Sciences as well as 29 Bahman Hospital affiliated to Tabriz Social Security Organization in 2016 using census sampling method. Three questionnaires were employed to collect demographic data and to explore the nurses' perceptions and knowledge of the NIDCAP program. Results Findings of the study indicated that the mean (SD) nurses' knowledge and perception scores for NIDCAP were 71.83 (1.64) and 76.80 (0.79), respectively. In the terms of knowledge, significant differences were found with regard to being married (t = -2.39, p < 0.019), having an MSc degree (t = -2.14, p = 0.034), and employment experience (t = -3.38, p = 0.001). However, there was no significant relationship between perception and demographic variables (p > 0.05), nor was there any significant relationship between perception and knowledge (p = 0.275). Conclusions The results of this study showed that the majority of nurses participating in the study had high knowledge about NIDCAP.", "gemini": "Bối cảnh Chương trình chăm sóc và đánh giá phát triển cá nhân cho trẻ sơ sinh (NIDCAP) là một phương pháp tiếp cận mới đa phương diện, hỗ trợ chức năng và phát triển sức khỏe đặc biệt ở trẻ sơ sinh non tháng tại khoa chăm sóc tích cực sơ sinh (NICU). Nghiên cứu này được thiết kế để đánh giá kiến thức và nhận thức của các y tá là thành viên quan trọng nhất trong nhóm đa ngành của NIDCAP về chương trình này. Vật liệu và phương pháp Nghiên cứu cắt ngang này được tiến hành trên 120 y tá làm việc tại các khoa NICU của bệnh viện Alzahra, Taleghani và Bệnh viện Nhi trực thuộc các trung tâm giáo dục và điều trị của Đại học Khoa học Y tế Tabriz cũng như Bệnh viện Bahman 29 trực thuộc Tổ chức An sinh Xã hội Tabriz vào năm 2016 sử dụng phương pháp lấy mẫu điều tra dân số. Ba bảng câu hỏi đã được sử dụng để thu thập dữ liệu nhân khẩu học và để khám phá nhận thức và kiến thức của các y tá về chương trình NIDCAP. Kết quả Kết quả nghiên cứu chỉ ra rằng điểm số kiến thức và nhận thức trung bình (SD) của các y tá đối với NIDCAP lần lượt là 71,83 (1,64) và 76,80 (0,79). Về mặt kiến thức, có sự khác biệt đáng kể liên quan đến việc đã kết hôn (t = -2,39, p < 0,019), có bằng Thạc sĩ (t = -2,14, p = 0,034) và kinh nghiệm làm việc (t = -3,38, p = 0,001). Tuy nhiên, không có mối quan hệ đáng kể nào giữa nhận thức và các biến nhân khẩu học (p > 0,05) cũng như không có mối quan hệ đáng kể nào giữa nhận thức và kiến thức (p = 0,275). Kết luận Kết quả nghiên cứu này cho thấy phần lớn các y tá tham gia nghiên cứu có kiến thức cao về NIDCAP."} +{"idx": 1900069, "en": "Background Critically ill patients admitted to intensive care units (ICUs) frequently experience pain, but the severity of pain in this group of patients is underestimated by the treatment team due to barriers to verbal communication. The aim of the present study was comparing the severity of pain measured by two scales: behavioral pain scale (BPS) and critical-care pain observation tool (CPOT) in ICU-admitted patients during routine daily procedures. Materials and Methods Ninety patients were enrolled in the study. The severity of pain was measured during resting, invasive (suctioning) and noninvasive (mouthwash and body position change) procedures, and respiratory physiotherapy with two scales: BPS and CPOT. Wilcoxon and Friedman statistical tests were used to compare the score of pain in different situations, and Spearman correlation coefficient was also used to measure the correlation of pain score measured by two scales. Results Patients experienced no pain during resting, mild pain during changing position, and respiratory physiotherapy, mild-to-moderate pain during mouthwash and moderate pain during secretion suctioning. Wilcoxon test used for pairwise comparisons between pain score in different situations showed a significant difference in both scales (p < 0.05). There were positive and strong correlations (r > 0.80, p < 0.05) between the pain score measured by BPS and CPOT from ICU-admitted patients in all procedures. Conclusions Critically ill patients in ICU experience a different range of pain in routine daily care. BPS and CPOT scales could be used successfully for monitoring of pain in this group of patients.", "gemini": "Bối cảnh Bệnh nhân bệnh nặng được đưa vào các đơn vị chăm sóc tích cực (ICU) thường xuyên bị đau, nhưng mức độ đau ở nhóm bệnh nhân này bị đánh giá thấp bởi đội ngũ điều trị do rào cản trong giao tiếp bằng lời nói. Mục đích của nghiên cứu hiện tại là so sánh mức độ đau được đo bằng hai thang: thang đo đau theo hành vi (BPS) và công cụ quan sát đau trong chăm sóc tích cực (CPOT) ở những bệnh nhân được đưa vào ICU trong các quy trình thường quy hàng ngày. Vật liệu và phương pháp Có chín mươi bệnh nhân được ghi danh vào nghiên cứu. Mức độ đau được đo trong khi nghỉ ngơi, các thủ thuật xâm lấn (hút dịch) và không xâm lấn (súc miệng và thay đổi tư thế cơ thể) và vật lý trị liệu hô hấp bằng hai thang đo: BPS và CPOT. Các kiểm tra thống kê Wilcoxon và Friedman được sử dụng để so sánh điểm đau trong các tình huống khác nhau và hệ số tương quan Spearman cũng được sử dụng để đo tương quan của điểm đau được đo bằng hai thang đo. Kết quả Bệnh nhân không đau khi nghỉ ngơi, đau nhẹ khi thay đổi tư thế và vật lý trị liệu hô hấp, đau nhẹ đến vừa khi súc miệng và đau vừa khi hút dịch tiết. Kiểm tra Wilcoxon được sử dụng để so sánh từng cặp giữa điểm đau trong các tình huống khác nhau cho thấy có sự khác biệt đáng kể ở cả hai thang đo (p < 0,05). Có mối tương quan tích cực và mạnh (r> 0,80, p < 0,05) giữa điểm đau được đo bằng BPS và CPOT từ những bệnh nhân được đưa vào ICU trong tất cả các thủ thuật. Kết luận Bệnh nhân bệnh nặng trong ICU trải qua các mức độ đau khác nhau trong quá trình chăm sóc hàng ngày theo quy định. Thang đo BPS và CPOT có thể được sử dụng thành công để theo dõi cơn đau ở nhóm bệnh nhân này."} +{"idx": 1900070, "en": "Background Because of the increasing number of the elderly, the need for care in aged nursing homes (NHs) is increasing. As a cultural perspective toward care is new, it is very important to understand the communication behavior of the elderly in various cultures. This study aimed to explore the communication behaviors among the elderly in NHs in Kerman, Iran. Materials and Methods This ethnographic focused study was conducted on 25 staff members and 8 residents in two NHs in 2015. Data were collected through observation of the participants, field notes, and semi-structured deep interviews in Kerman. Cuba and Lincoln method was used to ensure the trustworthiness of the data. The collected data were analyzed through content analysis. Results By analyzing primary codes, 3 categories and 8 subcategories were extracted through content analysis including fluctuation in communication (task-oriented communication, avoidance of communication, and establishment of effective communication), artificial collaboration (fake friendships, jealousy, and its consequences, and cooperation in concealing error), and lack of collaboration among team members (fragmented teamwork, extreme working conditions, and physical burnout among the elderly). Conclusions The results of this study indicate that communication problems between caregivers and the elderly are high. Thus, it is necessary that people who care for elderly indiviuals are trained regarding special communication strategies and skills, and they are not influenced by fatigue and burnout resulting from caring.", "gemini": "Bối cảnh Do số lượng người cao tuổi ngày càng tăng nên nhu cầu chăm sóc tại các viện dưỡng lão (NH) dành cho người già ngày càng tăng. Vì quan điểm văn hóa đối với việc chăm sóc là mới nên việc hiểu được hành vi giao tiếp của người cao tuổi ở các nền văn hóa khác nhau là rất quan trọng. Nghiên cứu này nhằm mục đích khám phá các hành vi giao tiếp giữa những người cao tuổi tại NH ở Kerman, Iran. Vật liệu và phương pháp Nghiên cứu tập trung dân tộc học này được tiến hành trên 25 nhân viên và 8 cư dân tại hai NH vào năm 2015. Dữ liệu được thu thập thông qua quan sát những người tham gia, ghi chép thực tế và phỏng vấn chuyên sâu có cấu trúc tại Kerman. Phương pháp Cuba và Lincoln được sử dụng để đảm bảo độ tin cậy của dữ liệu. Dữ liệu thu thập được được phân tích thông qua phân tích nội dung. Kết quả Bằng cách phân tích các mã chính, 3 loại và 8 tiểu loại đã được trích xuất thông qua phân tích nội dung bao gồm dao động trong giao tiếp (giao tiếp theo nhiệm vụ, tránh giao tiếp và thiết lập giao tiếp hiệu quả), hợp tác giả tạo (tình bạn giả, ghen tuông và hậu quả của nó, và hợp tác che giấu lỗi) và thiếu hợp tác giữa các thành viên trong nhóm (làm việc nhóm rời rạc, điều kiện làm việc khắc nghiệt và kiệt sức về thể chất ở người cao tuổi). Kết luận Kết quả nghiên cứu này chỉ ra rằng các vấn đề giao tiếp giữa những người chăm sóc và người cao tuổi là rất cao. Do đó, cần phải đào tạo những người chăm sóc những cá nhân cao tuổi về các chiến lược và kỹ năng giao tiếp đặc biệt và họ không bị ảnh hưởng bởi sự mệt mỏi và kiệt sức do việc chăm sóc."} +{"idx": 1900071, "en": "Background The family caregivers of patients undergoing hemodialysis are faced with multiple physical, psychological, social, economic, and spiritual problems that increase their care burden. The present study was conducted to determine the effects of a family-based training program on the care burden of family caregivers of patients undergoing hemodialysis. Materials and Methods The present controlled, randomized, clinical trial was conducted on 70 caregivers of patients undergoing hemodialysis in Ali Asghar and Zahray-e Marzieh hospitals in Isfahan, Iran, in 2017. After conducting convenient sampling, 70 participants were randomly assigned into 2 groups (35 in each group). The experimental group received the family-based training program and the control group received usual care plan. Data were collected using the Zarit Burden Scale before, immediately after, and 1 month after the intervention and were then analyzed by independent t-test, Chi-square, and Analysis of Covariance (ANCOVA) repeated measure. Results The results showed that both groups were homogeneous in terms of their demographic data and showed no significant differences. The main effect of group was significant, indicating a significant decrease in care burden in the experimental group after the intervention (F 1,67 = 1089, p < 0.001). However, the interaction of time and group was not significant, indicating insignificant difference in burden 1 month after intervention (p > 0.05). Conclusions Since the family-based training program successfully reduced the burden of care immediately after intervention, similar family-based training programs are recommended to be designed and developed. However, insignificant time effect suggests further researches of long time effects of such program.", "gemini": "Bối cảnh Những người chăm sóc gia đình của bệnh nhân đang chạy thận nhân tạo phải đối mặt với nhiều vấn đề về thể chất, tâm lý, xã hội, kinh tế và tinh thần làm tăng gánh nặng chăm sóc của họ. Nghiên cứu hiện tại được tiến hành để xác định hiệu quả của chương trình đào tạo dựa trên gia đình đối với gánh nặng chăm sóc của người chăm sóc gia đình của bệnh nhân đang chạy thận nhân tạo. Vật liệu và phương pháp Thử nghiệm lâm sàng có đối chứng, ngẫu nhiên hiện tại được tiến hành trên 70 người chăm sóc bệnh nhân đang chạy thận nhân tạo tại bệnh viện Ali Asghar và Zahray-e Marzieh ở Isfahan, Iran, vào năm 2017. Sau khi tiến hành lấy mẫu thuận tiện, 70 người tham gia được phân ngẫu nhiên vào 2 nhóm (35 người mỗi nhóm). Nhóm thực nghiệm nhận được chương trình đào tạo dựa trên gia đình và nhóm đối chứng nhận được kế hoạch chăm sóc thông thường. Dữ liệu được thu thập bằng Thang đo gánh nặng Zarit trước, ngay sau và 1 tháng sau khi can thiệp và sau đó được phân tích bằng kiểm định t độc lập, Chi-bình phương và Phân tích hiệp phương sai (ANCOVA) đo lặp lại. Kết quả Kết quả cho thấy cả hai nhóm đều đồng nhất về dữ liệu nhân khẩu học và không có sự khác biệt đáng kể. Hiệu ứng chính của nhóm là có ý nghĩa, cho thấy gánh nặng chăm sóc giảm đáng kể ở nhóm thực nghiệm sau khi can thiệp (F 1,67 = 1089, p < 0,001). Tuy nhiên, sự tương tác của thời gian và nhóm không đáng kể, cho thấy sự khác biệt không đáng kể về gánh nặng 1 tháng sau khi can thiệp (p> 0,05). Kết luận Vì chương trình đào tạo dựa trên gia đình đã giảm thành công gánh nặng chăm sóc ngay sau khi can thiệp, nên khuyến nghị thiết kế và phát triển các chương trình đào tạo dựa trên gia đình tương tự. Tuy nhiên, hiệu ứng thời gian không đáng kể cho thấy cần nghiên cứu thêm về các hiệu ứng lâu dài của chương trình như vậy."} +{"idx": 1900072, "en": "Background Drug dependence is one of the current problems that leads to the drug dependents persons' suffer and imposes a huge mental burden to their family members. This study aimed investigating to determine the effect of cognitive- behavioral therapy (CBT) on the burden of the caregivers of drug dependent people. In CBT, caregivers discover thought and behavioral mistakes and recover them. Materials and Methods This randomized controlled clinical trial was conducted on 64 caregivers of drug dependent individuals referring to one of the clinical and educational centers in Isfahan, Iran, which were randomly assigned to two equal groups (intervention and control) in 2016. Intervention group under went eight 90-min CBT sessions, whereas control group attended 3 group sessions to express their experiences. Data were collected by Zarit Burden scale and the demographic questionnaire and analyzed by independent t-test, Chi-square, and ANCOVA repeated measure through SPSS 18. Results Before the intervention, no statistical difference was observed among two groups in the mean scores of burden (t = 0.75, p = 0.46). There was a significant difference between three time points in the intervention group (F = 3.24, p < 0.001). However, care burden mean score did not show a significant reduction in the control group (F = 0.17, p = 0.96). Conclusions The CBT can lead to reduction of burden in drug dependent caregivers'. We suggest encouraging nurses to establish educational programs such as CBT to maintain and improve caregiver's mental health.", "gemini": "Bối cảnh Nghiện thuốc là một trong những vấn đề hiện tại dẫn đến người nghiện thuốc phải chịu đựng và gây gánh nặng tinh thần rất lớn cho các thành viên trong gia đình. Nghiên cứu này nhằm mục đích điều tra để xác định hiệu quả của liệu pháp hành vi nhận thức (CBT) đối với gánh nặng của những người chăm sóc người nghiện ma túy. Trong CBT, những người chăm sóc khám phá những sai lầm về suy nghĩ và hành vi và phục hồi chúng. Vật liệu và phương pháp Thử nghiệm lâm sàng có đối chứng ngẫu nhiên này được tiến hành trên 64 người chăm sóc những người nghiện ma túy đang giới thiệu đến một trong những trung tâm lâm sàng và giáo dục tại Isfahan, Iran, được phân ngẫu nhiên thành hai nhóm có số lượng bằng nhau (nhóm can thiệp và nhóm đối chứng) vào năm 2016. Nhóm can thiệp đã trải qua tám buổi CBT kéo dài 90 phút, trong khi nhóm đối chứng tham dự 3 buổi nhóm để bày tỏ kinh nghiệm của họ. Dữ liệu được thu thập bằng thang đo gánh nặng Zarit và bảng câu hỏi nhân khẩu học và được phân tích bằng kiểm định t độc lập, Chi bình phương và biện pháp lặp lại ANCOVA thông qua SPSS 18. Kết quả Trước khi can thiệp, không thấy sự khác biệt thống kê giữa hai nhóm về điểm trung bình của gánh nặng (t = 0,75, p = 0,46). Có sự khác biệt đáng kể giữa ba thời điểm trong nhóm can thiệp (F = 3,24, p < 0,001). Tuy nhiên, điểm trung bình gánh nặng chăm sóc không cho thấy sự giảm đáng kể trong nhóm đối chứng (F = 0,17, p = 0,96). Kết luận CBT có thể giúp giảm gánh nặng cho những người chăm sóc người nghiện ma túy. Chúng tôi đề nghị khuyến khích các y tá thiết lập các chương trình giáo dục như CBT để duy trì và cải thiện sức khỏe tinh thần của người chăm sóc."} +{"idx": 1900073, "en": "Locked twins is a rare and dangerous multiple pregnancy and generally occurs in about 1 in every 1000 twin births. A 23-year-old female with gravida 1, para 0 pregnancy was admitted. The head of the first fetus was locked to the chin of the second fetus (alive) at the top of the symphysis pubis. The uterus was relaxed with isoflurane. The legs and the back of the baby were extended to the symphysis pubis. The head of the second baby was gently positioned to the posterior walls of uterine cavity on the upper side of it. So, the locked head of the first baby was released and both fetuses were delivered vaginally. The second baby was in good condition. By recognizing the probable cases, radiological diagnosis, and punctual cesarean delivery, it is possible to avoid the fetal morbidity and mortality in locked twins.", "gemini": "Sinh đôi bị khóa là một dạng thai kỳ đôi hiếm gặp và nguy hiểm, thường xảy ra ở khoảng 1/1000 ca sinh đôi. Một phụ nữ 23 tuổi mang thai lần 1, sinh thường 0 lần đã được nhập viện. Đầu của thai nhi thứ nhất bị khóa vào cằm của thai nhi thứ hai (còn sống) ở vị trí trên cùng của khớp mu. Tử cung được làm giãn bằng isoflurane. Hai chân và lưng của em bé được kéo dài đến khớp mu. Đầu của em bé thứ hai được nhẹ nhàng đưa vào thành sau của buồng tử cung ở phía trên của nó. Vì vậy, đầu bị khóa của em bé thứ nhất đã được giải phóng và cả hai thai nhi đều được sinh ra qua ngả âm đạo. Em bé thứ hai trong tình trạng tốt. Bằng cách nhận biết các trường hợp có thể xảy ra, chẩn đoán hình ảnh và sinh mổ đúng thời điểm, có thể tránh được tình trạng tử vong và bệnh tật ở thai đôi bị khóa."} +{"idx": 1900074, "en": "This guide provides an overview of social media (SoMe) use with the recent updates for the \"cytopathologists and the ones interested in\". Our aim was to introduce the basic terms and rules, the potential benefits and risks, and some tips and tricks for using SoMe. The two most popular SoMe platforms, Facebook and Twitter, were the focus of this article. Thus far, many pathologists have already proved how efficiently the SoMe services could be utilized; the same applies specifically to the community of cytopathology. In our opinion, the more CPs are involved in SoMe, the more connected, productive, and stronger the community will become.", "gemini": "Hướng dẫn này cung cấp tổng quan về việc sử dụng phương tiện truyền thông xã hội (SoMe) với các cập nhật gần đây cho \"các nhà tế bào bệnh học và những người quan tâm\". Mục đích của chúng tôi là giới thiệu các thuật ngữ và quy tắc cơ bản, những lợi ích và rủi ro tiềm ẩn, cùng một số mẹo và thủ thuật để sử dụng SoMe. Hai nền tảng SoMe phổ biến nhất là Facebook và Twitter là trọng tâm của bài viết này. Cho đến nay, nhiều nhà nghiên cứu bệnh học đã chứng minh hiệu quả sử dụng các dịch vụ SoMe; điều tương tự cũng áp dụng cụ thể cho cộng đồng tế bào bệnh học. Theo chúng tôi, càng có nhiều CP tham gia vào SoMe, cộng đồng sẽ càng trở nên kết nối, hiệu quả và mạnh mẽ hơn."} +{"idx": 1900075, "en": "Background The fitness of holometabolous insects depends largely on resources acquired at the larval stage. Larval density is an important factor modulating larval resource-acquisition, influencing adult survival, reproduction, and population maintenance. To date, however, our understanding of how larval crowding affects adult physiology and behaviour is limited, and little is known about how larval crowding affects adult non-reproductive ecological traits. Here, larval density in the rearing environment of the polyphagous fruit fly Bactrocera tryoni ('Queensland fruit-fly') was manipulated to generate crowded and uncrowded larval treatments. The effects of larval crowding on pupal weight, adult emergence, adult body weight, energetic reserves, fecundity, feeding patterns, flight ability, as well as adult predation risk were investigated. Results Adults from the crowded larval treatment had lower adult emergence, body weight, energetic reserves, flight ability and fecundity compared to adults from the uncrowded larval treatment. Adults from the crowded larval treatment had greater total food consumption (i.e., consumption of yeast plus sucrose) relative to body weight for both sexes compared to adults from the uncrowded treatment. Furthermore, males from the crowded treatment consumed more yeast relative to their body weight than males from the uncrowded treatment, while females from the crowded treatment consumed more sucrose relative to their body weight than females from the uncrowded treatment. Importantly, an interaction between the relative consumptions of sucrose and yeast and sex revealed that the density of conspecifics in the developmental environment differentially affects feeding of adult males and females. We found no effect of larval treatment on adult predation probability. However, males were significantly more likely to be captured by ants than females. Conclusion We show that larvae crowding can have important implications to ecological traits in a polyphagous fly, including traits such as adult energetic reserve, flight ability, and adult sex-specific nutrient intake. Our findings contextualise the effects of larval developmental conditions into a broad ecological framework, hence providing a better understanding of their significance to adult behaviour and fitness. Furthermore, the knowledge presented here can help us better understanding downstream density-dependent effects of mass rearing conditions of this species, with potential relevance to Sterile Insect Technique.", "gemini": "Bối cảnh Sự khỏe mạnh của côn trùng biến thái hoàn toàn phụ thuộc phần lớn vào nguồn tài nguyên thu được ở giai đoạn ấu trùng. Mật độ ấu trùng là một yếu tố quan trọng điều chỉnh việc thu nhận nguồn lực của ấu trùng, ảnh hưởng đến sự sống sót của con trưởng thành, khả năng sinh sản và duy trì quần thể. Tuy nhiên, cho đến nay, hiểu biết của chúng ta về cách thức chen chúc ấu trùng ảnh hưởng đến sinh lý và hành vi của con trưởng thành còn hạn chế và chúng ta biết rất ít về cách thức chen chúc ấu trùng ảnh hưởng đến các đặc điểm sinh thái không sinh sản ở con trưởng thành. Ở đây, mật độ ấu trùng trong môi trường nuôi của ruồi ăn quả đa thực Bactrocera tryoni ('ruồi ăn quả Queensland') đã được thao tác để tạo ra các phương pháp xử lý ấu trùng đông đúc và không đông đúc. Các tác động của việc chen chúc ấu trùng đối với trọng lượng nhộng, sự xuất hiện của con trưởng thành, trọng lượng cơ thể của con trưởng thành, dự trữ năng lượng, khả năng sinh sản, kiểu ăn, khả năng bay cũng như nguy cơ bị săn mồi ở con trưởng thành đã được nghiên cứu. Kết quả Con trưởng thành từ phương pháp xử lý ấu trùng đông đúc có sự xuất hiện của con trưởng thành thấp hơn, trọng lượng cơ thể, dự trữ năng lượng, khả năng bay và khả năng sinh sản so với con trưởng thành từ phương pháp xử lý ấu trùng không đông đúc. Con trưởng thành từ phương pháp xử lý ấu trùng đông đúc có tổng lượng thức ăn tiêu thụ lớn hơn (tức là tiêu thụ men cộng với sucrose) so với trọng lượng cơ thể đối với cả hai giới so với con trưởng thành từ phương pháp xử lý không đông đúc. Hơn nữa, con đực từ phương pháp xử lý đông đúc tiêu thụ nhiều men hơn so với trọng lượng cơ thể của chúng so với con đực từ phương pháp xử lý không đông đúc, trong khi con cái từ phương pháp xử lý đông đúc tiêu thụ nhiều sucrose hơn so với trọng lượng cơ thể của chúng so với con cái từ phương pháp xử lý không đông đúc. Điều quan trọng là sự tương tác giữa lượng tiêu thụ sucrose và men tương đối và giới tính cho thấy rằng mật độ của những cá thể cùng loài trong môi trường phát triển ảnh hưởng khác nhau đến việc ăn của con đực và con cái trưởng thành. Chúng tôi không tìm thấy tác động của phương pháp xử lý ấu trùng đối với khả năng bị săn mồi ở con trưởng thành. Tuy nhiên, con đực có khả năng bị kiến bắt cao hơn đáng kể so với con cái. Kết luận Chúng tôi chỉ ra rằng việc chen chúc ấu trùng có thể có ý nghĩa quan trọng đối với các đặc điểm sinh thái ở ruồi ăn tạp, bao gồm các đặc điểm như dự trữ năng lượng của con trưởng thành, khả năng bay và lượng dinh dưỡng cụ thể theo giới tính của con trưởng thành. Những phát hiện của chúng tôi ngữ cảnh hóa các tác động của điều kiện phát triển của ấu trùng vào một khuôn khổ sinh thái rộng, do đó cung cấp sự hiểu biết tốt hơn về ý nghĩa của chúng đối với hành vi và thể lực của con trưởng thành. Hơn nữa, kiến thức được trình bày ở đây có thể giúp chúng ta hiểu rõ hơn về các tác động phụ thuộc vào mật độ hạ nguồn của các điều kiện nuôi tập trung của loài này, có thể liên quan đến Kỹ thuật côn trùng vô trùng."} +{"idx": 1900076, "en": "Background Previous analyses of factors influencing footfall timings and gait selection in quadrupeds have focused on the implications for energetic cost or gait mechanics separately. Here we present a model for symmetrical walking gaits in quadrupedal mammals that combines both factors, and aims to predict the substrate contexts in which animals will select certain ranges of footfall timings that (1) minimize energetic cost, (2) minimize rolling and pitching moments, or (3) balance the two. We hypothesize that energy recovery will be a priority on all surfaces, and will be the dominant factor determining footfall timings on flat, ground-like surfaces. The ability to resist pitch and roll, however, will play a larger role in determining footfall choice on narrower and more complex branch-like substrates. As a preliminary test of the expectations of the model, we collected sample data on footfall timings in a primate with relatively high flexibility in footfall timings - the squirrel monkey (Saimiri sciureus) - walking on a flat surface, straight pole, and a pole with laterally-projecting branches to simulate simplified ground and branch substrates. We compare limb phase values on these supports to the expectations of the model. Results As predicted, walking steps on the flat surface tended towards limb phase values that promote energy exchange. Both pole substrates induced limb phase values predicted to favor reduced pitching and rolling moments. Conclusions These data provide novel insight into the ways in which animals may choose to adjust their behavior in response to movement on flat versus complex substrates and the competing selective factors that influence footfall timing in mammals. These data further suggest a pathway for future investigations using this perspective.", "gemini": "Bối cảnh Các phân tích trước đây về các yếu tố ảnh hưởng đến thời gian đặt chân và lựa chọn dáng đi ở động vật bốn chân tập trung vào các tác động đối với chi phí năng lượng hoặc cơ chế dáng đi riêng biệt. Ở đây, chúng tôi trình bày một mô hình cho các dáng đi đối xứng ở động vật có vú bốn chân kết hợp cả hai yếu tố và nhằm mục đích dự đoán các bối cảnh cơ chất mà động vật sẽ chọn các phạm vi thời gian đặt chân nhất định để (1) giảm thiểu chi phí năng lượng, (2) giảm thiểu các lực lăn và nghiêng, hoặc (3) cân bằng cả hai. Chúng tôi đưa ra giả thuyết rằng việc phục hồi năng lượng sẽ là ưu tiên trên mọi bề mặt và sẽ là yếu tố chi phối quyết định thời gian đặt chân trên các bề mặt phẳng giống mặt đất. Tuy nhiên, khả năng chống lại độ nghiêng và lăn sẽ đóng vai trò lớn hơn trong việc xác định lựa chọn đặt chân trên các bề mặt giống cành hẹp hơn và phức tạp hơn. Là một thử nghiệm sơ bộ về kỳ vọng của mô hình, chúng tôi đã thu thập dữ liệu mẫu về thời gian đặt chân ở một loài linh trưởng có tính linh hoạt tương đối cao trong thời gian đặt chân - khỉ sóc (Saimiri sciureus) - đi trên bề mặt phẳng, cột thẳng và một cột có các nhánh nhô ra bên để mô phỏng các bề mặt đất và cành được đơn giản hóa. Chúng tôi so sánh các giá trị pha chi trên các giá đỡ này với các kỳ vọng của mô hình. Kết quả Theo dự đoán, các bước đi trên bề mặt phẳng có xu hướng hướng đến các giá trị pha chi thúc đẩy trao đổi năng lượng. Cả hai giá thể cực đều gây ra các giá trị pha chi được dự đoán là có lợi cho các lực nghiêng và lăn giảm. Kết luận Những dữ liệu này cung cấp cái nhìn sâu sắc mới về cách thức mà động vật có thể lựa chọn điều chỉnh hành vi của mình để đáp ứng với chuyển động trên các bề mặt phẳng so với phức tạp và các yếu tố chọn lọc cạnh tranh ảnh hưởng đến thời gian đặt chân ở động vật có vú. Những dữ liệu này tiếp tục gợi ý một con đường cho các cuộc điều tra trong tương lai sử dụng góc nhìn này."} +{"idx": 1900077, "en": "Background Iron is a critical component of metabolic homeostasis, but consumption of dietary iron has increased dramatically in the last 30 years, corresponding with the rise of metabolic disease. While the link between iron metabolism and metabolic health is well established, the extent to which dietary iron contributes to metabolic disease risk is unexplored. Further, it is unknown how dietary iron interacts with genetic background to modify metabolic disease risk. Methods LG/J and SM/J inbred mouse strains were used to investigate the relationship between genetic background and metabolic function during an 8-week high iron diet. Glucose tolerance and adiposity were assessed, colorimetric assays determined levels of circulating metabolic markers, and hepatic iron content was measured. RNA sequencing was performed on white adipose tissue to identify genes differentially expressed across strain, diet, and strain X diet cohorts. Hepatic Hamp expression and circulating hepcidin was measured, and small nucleotide variants were identified in the Hamp genic region. Results LG/J mice experienced elevated fasting glucose and glucose intolerance during the high iron diet, corresponding with increased hepatic iron load, increased circulating ferritin, and signs of liver injury. Adipose function was also altered in high iron-fed LG/J mice, including decreased adiposity and leptin production and differential expression of genes involved in iron and glucose homeostasis. LG/J mice failed to upregulate hepatic Hamp expression during the high iron diet, resulting in low circulating hepcidin levels compared to SM/J mice. Conclusions This study highlights the importance of accounting for genetic variation when assessing the effects of diet on metabolic health, and suggests dietary iron's impact on liver and adipose tissue is an underappreciated component of metabolic disease risk.", "gemini": "Bối cảnh Sắt là một thành phần quan trọng của cân bằng nội môi trao đổi chất, nhưng lượng sắt tiêu thụ trong chế độ ăn uống đã tăng đáng kể trong 30 năm qua, tương ứng với sự gia tăng của bệnh chuyển hóa. Mặc dù mối liên hệ giữa chuyển hóa sắt và sức khỏe trao đổi chất đã được thiết lập rõ ràng, nhưng mức độ mà sắt trong chế độ ăn uống góp phần vào nguy cơ mắc bệnh chuyển hóa vẫn chưa được khám phá. Hơn nữa, không rõ sắt trong chế độ ăn tương tác với nền tảng di truyền như thế nào để sửa đổi nguy cơ mắc bệnh chuyển hóa. Phương pháp Các dòng chuột cận huyết LG/J và SM/J đã được sử dụng để điều tra mối quan hệ giữa nền tảng di truyền và chức năng trao đổi chất trong chế độ ăn nhiều sắt kéo dài 8 tuần. Dung nạp glucose và tình trạng béo phì đã được đánh giá, các xét nghiệm sắc ký màu xác định mức độ các chất đánh dấu chuyển hóa lưu hành và hàm lượng sắt trong gan được đo. Trình tự RNA đã được thực hiện trên mô mỡ trắng để xác định các gen biểu hiện khác nhau giữa các nhóm đồng loại, chế độ ăn uống và chế độ ăn uống X. Biểu hiện Hamp ở gan và hepcidin lưu hành đã được đo và các biến thể nucleotide nhỏ đã được xác định trong vùng gen Hamp. Kết quả Chuột LG/J có lượng glucose lúc đói tăng cao và không dung nạp glucose trong chế độ ăn nhiều sắt, tương ứng với tải sắt ở gan tăng, ferritin lưu hành tăng và các dấu hiệu tổn thương gan. Chức năng mỡ cũng bị thay đổi ở những con chuột LG/J ăn nhiều sắt, bao gồm tình trạng béo phì và sản xuất leptin giảm và biểu hiện khác biệt của các gen liên quan đến cân bằng sắt và glucose. Chuột LG/J không điều chỉnh tăng biểu hiện Hamp ở gan trong chế độ ăn nhiều sắt, dẫn đến mức hepcidin lưu hành thấp so với chuột SM/J. Kết luận Nghiên cứu này nhấn mạnh tầm quan trọng của việc tính đến biến thể di truyền khi đánh giá tác động của chế độ ăn uống đối với sức khỏe trao đổi chất và cho thấy tác động của sắt trong chế độ ăn uống đối với gan và mô mỡ là một thành phần chưa được đánh giá đúng về nguy cơ mắc bệnh chuyển hóa."} +{"idx": 1900078, "en": "Background Kangaroo mother care is a comprehensive intervention given for all newborns especially for premature and low birthweight infants. It is the most feasible and preferred intervention for decreasing neonatal morbidity and mortality. Even though time to initiating breastfeeding has been examined by randomized controlled trials, varying findings have been reported. Therefore, the main objective of this meta-analysis was to estimate the pooled mean time to initiate breastfeeding among preterm and low birthweight infants. Methods The authors searched for randomized controlled trial studies conducted on the effects of kangaroo mother care on the time to breastfeeding initiation among preterm and low birthweight infants. Published articles were identified through a computerized search of electronic databases that includes MEDLINE via PubMed, EMBASE, CINAHL and CENTRAL. The search terms were kangaroo mother care or (skin to skin), or conventional care, newborns, preterm infants, low birthweight infants and randomized controlled trial. A total of 467 eligible titles were identified and eight studies met the inclusion criteria. The extracted data were entered and analyzed using Cochrane Review Manager-5-3 software. Heterogeneity across studies was evaluated by Chi2 test and inconsistency index (I2). Publication bias was assessed using a funnel plot. The random effect model was applied to estimate the pooled mean time to initiate breastfeeding with 95% confidence interval. Results In this meta-analysis, the overall pooled mean time to initiate breastfeeding was 2.6 days (95% CI 1.23, 3.96). Preterm and low birthweight infants receiving kangaroo mother care intervention initiated breastfeeding 2 days 14 h 24 min earlier than conventional care of radiant warmer/incubator method. Conclusions Kangaroo mother care promotes early initiation of breastfeeding as compared to conventional care method. Therefore, health facilities need to implement the kangaroo mother care for preterm and low birthweight infants.", "gemini": "Bối cảnh Chăm sóc mẹ Kangaroo là một biện pháp can thiệp toàn diện dành cho tất cả trẻ sơ sinh, đặc biệt là trẻ sinh non và trẻ nhẹ cân. Đây là biện pháp can thiệp khả thi và được ưa chuộng nhất để giảm tỷ lệ mắc bệnh và tử vong ở trẻ sơ sinh. Mặc dù thời gian bắt đầu cho trẻ bú mẹ đã được kiểm tra bằng các thử nghiệm đối chứng ngẫu nhiên, nhưng các phát hiện khác nhau đã được báo cáo. Do đó, mục tiêu chính của phân tích tổng hợp này là ước tính thời gian trung bình gộp để bắt đầu cho trẻ bú mẹ ở trẻ sinh non và trẻ nhẹ cân. Phương pháp Các tác giả đã tìm kiếm các nghiên cứu thử nghiệm đối chứng ngẫu nhiên được tiến hành về tác động của việc chăm sóc mẹ Kangaroo đối với thời gian bắt đầu cho trẻ bú mẹ ở trẻ sinh non và trẻ nhẹ cân. Các bài báo được công bố đã được xác định thông qua tìm kiếm trên máy tính các cơ sở dữ liệu điện tử bao gồm MEDLINE qua PubMed, EMBASE, CINAHL và CENTRAL. Các thuật ngữ tìm kiếm là chăm sóc mẹ Kangaroo hoặc (da kề da), hoặc chăm sóc thông thường, trẻ sơ sinh, trẻ sinh non, trẻ nhẹ cân và thử nghiệm đối chứng ngẫu nhiên. Tổng cộng 467 tiêu đề đủ điều kiện đã được xác định và tám nghiên cứu đáp ứng các tiêu chí đưa vào. Dữ liệu được trích xuất đã được nhập và phân tích bằng phần mềm Cochrane Review Manager-5-3. Độ không đồng nhất giữa các nghiên cứu được đánh giá bằng kiểm tra Chi2 và chỉ số không nhất quán (I2). Độ lệch công bố được đánh giá bằng biểu đồ phễu. Mô hình hiệu ứng ngẫu nhiên được áp dụng để ước tính thời gian trung bình gộp để bắt đầu cho trẻ bú mẹ với khoảng tin cậy 95%. Kết quả Trong phân tích tổng hợp này, thời gian trung bình gộp chung để bắt đầu cho trẻ bú mẹ là 2,6 ngày (95% CI 1,23, 3,96). Trẻ sinh non và trẻ nhẹ cân được can thiệp chăm sóc mẹ Kangaroo bắt đầu bú mẹ sớm hơn 2 ngày 14 giờ 24 phút so với phương pháp chăm sóc thông thường bằng lò sưởi/lồng ấp. Kết luận Chăm sóc mẹ Kangaroo thúc đẩy việc bắt đầu cho trẻ bú mẹ sớm hơn so với phương pháp chăm sóc thông thường. Do đó, các cơ sở y tế cần triển khai chăm sóc mẹ Kangaroo cho trẻ sinh non và trẻ nhẹ cân."} +{"idx": 1900079, "en": "The difficult laparoscopic cholecystectomy remains a surgical challenge for surgeons who must decide between laparoscopic continuation and open conversion. The balance between the lack of open surgery training of young surgeons and the risk of maintaining the laparoscopic approach in difficult laparoscopic cholecystectomy is still an unresolved problem. Furthermore, the time that must be spent in an attempt to complete laparoscopic surgery before conversion is still controversial. The authors in this letter discuss about these and other questions that still require an answer.", "gemini": "Phẫu thuật cắt túi mật nội soi khó vẫn là một thách thức ngoại khoa đối với các bác sĩ phẫu thuật, những người phải quyết định giữa việc tiếp tục nội soi và chuyển đổi mở. Sự cân bằng giữa việc thiếu đào tạo phẫu thuật mở của các bác sĩ phẫu thuật trẻ và nguy cơ duy trì phương pháp nội soi trong phẫu thuật cắt túi mật nội soi khó vẫn là một vấn đề chưa được giải quyết. Hơn nữa, thời gian phải dành để cố gắng hoàn thành phẫu thuật nội soi trước khi chuyển đổi vẫn còn gây tranh cãi. Các tác giả trong lá thư này thảo luận về những câu hỏi này và các câu hỏi khác vẫn cần được trả lời."} +{"idx": 1900080, "en": "Background Mindfulness-based interventions are increasingly being implemented worldwide for problems with depression and anxiety, and they have shown evidence of efficacy. However, few studies have examined the effects of a mindfulness-based group therapy based on standard programs for depression and anxiety until follow-up in Japan. This study addresses that gap. Furthermore, this study explored the mechanisms of action, focusing on mindfulness, mind wandering, self-compassion, and the behavioral inhibition and behavioral activation systems (BIS/BAS) as possible mediators. Methods We examined 16 people who suffered from depression and/or anxiety in an 8-week mindfulness group therapy. Measurements were conducted using questionnaires on depression and trait-anxiety (outcome variables), mindfulness, mind wandering, self-compassion, and the BIS/BAS (process variables) at pre- and post-intervention and 2-month follow-up. Changes in the outcome and process variables were tested, and the correlations among the changes in those variables were explored. Results Depression and anxiety decreased significantly, with moderate to large effect sizes, from pre- to post-intervention and follow-up. In process variables, the observing and nonreactivity facets of mindfulness significantly increased from pre- to post-intervention and follow-up. The nonjudging facet of mindfulness and self-compassion significantly increased from pre-intervention to follow-up. Other facets of mindfulness, mind wandering, and the BIS/BAS did not significantly change. Improvements in some facets of mindfulness and self-compassion and reductions in BIS were significantly correlated with decreases in depression and anxiety. Conclusions An 8-week mindfulness group therapy program may be effective for people suffering from depression and anxiety in Japan. Mindfulness and self-compassion may be important mediators of the effects of the mindfulness group therapy. Future studies should confirm these findings by using a control group. Trial registration University Hospital Medical Information Network (UMIN CTR) UMIN000022966. Registered July 1, 2016, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000026425.", "gemini": "Bối cảnh Các biện pháp can thiệp dựa trên chánh niệm ngày càng được triển khai trên toàn thế giới để giải quyết các vấn đề về trầm cảm và lo âu, và chúng đã cho thấy bằng chứng về hiệu quả. Tuy nhiên, rất ít nghiên cứu đã kiểm tra tác động của liệu pháp nhóm dựa trên chánh niệm dựa trên các chương trình tiêu chuẩn về trầm cảm và lo âu cho đến khi theo dõi tại Nhật Bản. Nghiên cứu này giải quyết khoảng cách đó. Hơn nữa, nghiên cứu này đã khám phá các cơ chế hoạt động, tập trung vào chánh niệm, sự lang thang của tâm trí, lòng trắc ẩn và các hệ thống ức chế hành vi và kích hoạt hành vi (BIS/BAS) như những người trung gian có thể có. Phương pháp Chúng tôi đã kiểm tra 16 người bị trầm cảm và/hoặc lo lắng trong liệu pháp nhóm chánh niệm kéo dài 8 tuần. Các phép đo được thực hiện bằng cách sử dụng các bảng câu hỏi về trầm cảm và lo âu đặc điểm (biến kết quả), chánh niệm, sự lang thang của tâm trí, lòng trắc ẩn và BIS/BAS (biến quá trình) trước và sau can thiệp và theo dõi sau 2 tháng. Các thay đổi về kết quả và biến quá trình đã được kiểm tra và các mối tương quan giữa các thay đổi trong các biến đó đã được khám phá. Kết quả Trầm cảm và lo lắng giảm đáng kể, với quy mô hiệu ứng từ trung bình đến lớn, từ trước can thiệp đến sau can thiệp và theo dõi. Trong các biến quá trình, các khía cạnh quan sát và không phản ứng của chánh niệm tăng đáng kể từ trước can thiệp đến sau can thiệp và theo dõi. Khía cạnh không phán xét của chánh niệm và lòng trắc ẩn tăng đáng kể từ trước can thiệp đến khi theo dõi. Các khía cạnh khác của chánh niệm, sự lang thang của tâm trí và BIS/BAS không thay đổi đáng kể. Sự cải thiện ở một số khía cạnh của chánh niệm và lòng trắc ẩn và sự giảm BIS có tương quan đáng kể với sự giảm trầm cảm và lo âu. Kết luận Một chương trình liệu pháp nhóm chánh niệm kéo dài 8 tuần có thể có hiệu quả đối với những người bị trầm cảm và lo lắng ở Nhật Bản. Chánh niệm và lòng trắc ẩn có thể là những người trung gian quan trọng của các tác động của liệu pháp nhóm chánh niệm. Các nghiên cứu trong tương lai nên xác nhận những phát hiện này bằng cách sử dụng nhóm đối chứng. Đăng ký thử nghiệm Mạng thông tin y tế của Bệnh viện Đại học (UMIN CTR) UMIN000022966. Đã đăng ký ngày 1 tháng 7 năm 2016, https://upload.umin.ac.jp/cgi-open-bin/ctr/ctr_view.cgi?recptno=R000026425."} +{"idx": 1900081, "en": "Board games are played by moving game pieces in particular ways on special boards marked with patterns. To clarify the possible roles of board game use in psychosomatic medicine, the present review evaluated studies that investigated the effects of this activity on health education and treatment. A literature search conducted between January 2012 and August 2018 identified 83 relevant articles; 56 (67%) targeted education or training for health-related problems, six (7%) examined basic brain mechanisms, five (6%) evaluated preventative measures for dementia or contributions to healthy aging, and three (4%) assessed social communication or public health policies. The results of several randomized controlled trials indicated that the playing of traditional board games (e.g., chess, Go, and Shogi) helps to improve cognitive impairment and depression, and that the playing of newly developed board games is beneficial for behavioral modifications, such as the promotion of healthy eating, smoking cessation, and safe sex. Although the number of studies that have evaluated board game use in terms of mental health remains limited, many studies have provided interesting findings regarding brain function, cognitive effects, and the modification of health-related lifestyle factors.", "gemini": "Trò chơi trên bàn được chơi bằng cách di chuyển các quân cờ theo những cách cụ thể trên các bàn cờ đặc biệt được đánh dấu bằng các mẫu. Để làm rõ những vai trò có thể có của việc sử dụng trò chơi trên bàn trong y học tâm lý, bài đánh giá hiện tại đã đánh giá các nghiên cứu điều tra tác động của hoạt động này đối với giáo dục và điều trị sức khỏe. Một tìm kiếm tài liệu được tiến hành từ tháng 1 năm 2012 đến tháng 8 năm 2018 đã xác định được 83 bài báo có liên quan; 56 (67%) nhắm mục tiêu giáo dục hoặc đào tạo về các vấn đề liên quan đến sức khỏe, sáu (7%) xem xét các cơ chế não cơ bản, năm (6%) đánh giá các biện pháp phòng ngừa cho chứng mất trí hoặc đóng góp cho sự lão hóa khỏe mạnh và ba (4%) đánh giá các chính sách giao tiếp xã hội hoặc sức khỏe cộng đồng. Kết quả của một số thử nghiệm có đối chứng ngẫu nhiên cho thấy việc chơi các trò chơi trên bàn truyền thống (ví dụ: cờ vua, cờ vây và cờ tướng) giúp cải thiện suy giảm nhận thức và trầm cảm, và việc chơi các trò chơi trên bàn mới được phát triển có lợi cho việc thay đổi hành vi, chẳng hạn như thúc đẩy ăn uống lành mạnh, cai thuốc lá và quan hệ tình dục an toàn. Mặc dù số lượng các nghiên cứu đã đánh giá việc sử dụng trò chơi trên bàn về sức khỏe tâm thần vẫn còn hạn chế, nhưng nhiều nghiên cứu đã cung cấp những phát hiện thú vị liên quan đến chức năng não, hiệu ứng nhận thức và việc thay đổi các yếu tố lối sống liên quan đến sức khỏe."} +{"idx": 1900082, "en": "Background Enzymatic hydrolysis is a major step for cellulosic ethanol production. A thorough understanding of enzymatic hydrolysis is necessary to help design optimal conditions and economical systems. The original HCH-1 (Holtzapple-Caram-Humphrey-1) model is a generalized mechanistic model for enzymatic cellulose hydrolysis, but was previously applied only to the initial rates. In this study, the original HCH-1 model was modified to describe integrated enzymatic cellulose hydrolysis. The relationships between parameters in the HCH-1 model and substrate conversion were investigated. Literature models for long-term (> 48 h) enzymatic hydrolysis were summarized and compared to the modified HCH-1 model. Results A modified HCH-1 model was developed for long-term (> 48 h) enzymatic cellulose hydrolysis. This modified HCH-1 model includes the following additional considerations: (1) relationships between coefficients and substrate conversion, and (2) enzyme stability. Parameter estimation was performed with 10-day experimental data using α-cellulose as substrate. The developed model satisfactorily describes integrated cellulose hydrolysis data taken with various reaction conditions (initial substrate concentration, initial product concentration, enzyme loading, time). Mechanistic (and semi-mechanistic) literature models for long-term enzymatic hydrolysis were compared with the modified HCH-1 model and evaluated by the corrected version of the Akaike information criterion. Comparison results show that the modified HCH-1 model provides the best fit for enzymatic cellulose hydrolysis. Conclusions The HCH-1 model was modified to extend its application to integrated enzymatic hydrolysis; it performed well when predicting 10-day cellulose hydrolysis at various experimental conditions. Comparison with the literature models showed that the modified HCH-1 model provided the best fit.", "gemini": "Bối cảnh Thủy phân bằng enzym là một bước chính để sản xuất ethanol từ xenluloza. Việc hiểu rõ về thủy phân bằng enzym là cần thiết để giúp thiết kế các điều kiện tối ưu và hệ thống kinh tế. Mô hình HCH-1 (Holtzapple-Caram-Humphrey-1) ban đầu là một mô hình cơ học tổng quát cho quá trình thủy phân xenluloza bằng enzym, nhưng trước đây chỉ được áp dụng cho các tốc độ ban đầu. Trong nghiên cứu này, mô hình HCH-1 ban đầu đã được sửa đổi để mô tả quá trình thủy phân xenluloza bằng enzym tích hợp. Các mối quan hệ giữa các thông số trong mô hình HCH-1 và quá trình chuyển đổi cơ chất đã được nghiên cứu. Các mô hình tài liệu cho quá trình thủy phân bằng enzym dài hạn (> 48 giờ) đã được tóm tắt và so sánh với mô hình HCH-1 đã sửa đổi. Kết quả Một mô hình HCH-1 đã sửa đổi đã được phát triển cho quá trình thủy phân xenluloza bằng enzym dài hạn (> 48 giờ). Mô hình HCH-1 đã sửa đổi này bao gồm các cân nhắc bổ sung sau: (1) mối quan hệ giữa các hệ số và quá trình chuyển đổi cơ chất và (2) độ ổn định của enzyme. Ước tính thông số được thực hiện với dữ liệu thực nghiệm trong 10 ngày sử dụng α-xenluloza làm cơ chất. Mô hình đã phát triển mô tả một cách thỏa đáng dữ liệu thủy phân xenluloza tích hợp được lấy với các điều kiện phản ứng khác nhau (nồng độ cơ chất ban đầu, nồng độ sản phẩm ban đầu, lượng enzyme, thời gian). Các mô hình tài liệu cơ học (và bán cơ học) cho quá trình thủy phân bằng enzym dài hạn đã được so sánh với mô hình HCH-1 đã sửa đổi và được đánh giá bằng phiên bản đã sửa của tiêu chuẩn thông tin Akaike. Kết quả so sánh cho thấy mô hình HCH-1 đã sửa đổi cung cấp sự phù hợp tốt nhất cho quá trình thủy phân xenluloza bằng enzym. Kết luận Mô hình HCH-1 đã được sửa đổi để mở rộng ứng dụng của nó cho quá trình thủy phân bằng enzym tích hợp; nó hoạt động tốt khi dự đoán quá trình thủy phân xenluloza trong 10 ngày ở các điều kiện thực nghiệm khác nhau. So sánh với các mô hình tài liệu cho thấy mô hình HCH-1 đã sửa đổi cung cấp sự phù hợp tốt nhất."} +{"idx": 1900083, "en": "Over recent years, the development of new antibiotics has not kept pace with the rate at which bacteria develop resistance to these drugs. For this reason, many research groups have begun to design and study alternative therapeutics, including molecules to specifically inhibit the virulence of pathogenic bacteria. Because many of these pathogenic bacteria release protein toxins, which cause or exacerbate disease, inhibition of the activity of bacterial toxins is a promising anti-virulence strategy. In this review, we describe several approaches to inhibit the initial interactions of bacterial toxins with host cell membrane components. The mechanisms by which toxins interact with the host cell membrane components have been well-studied over the years, leading to the identification of therapeutic targets, which have been exploited in the work described here. We review efforts to inhibit binding to protein receptors and essential membrane lipid components, complex assembly, and pore formation. Although none of these molecules have yet been demonstrated in clinical trials, the in vitro and in vivo results presented here demonstrate their promise as novel alternatives and/or complements to traditional antibiotics.", "gemini": "Trong những năm gần đây, sự phát triển của các loại kháng sinh mới không theo kịp tốc độ mà vi khuẩn phát triển khả năng kháng thuốc. Vì lý do này, nhiều nhóm nghiên cứu đã bắt đầu thiết kế và nghiên cứu các phương pháp điều trị thay thế, bao gồm các phân tử để ức chế đặc hiệu độc lực của vi khuẩn gây bệnh. Vì nhiều loại vi khuẩn gây bệnh này giải phóng độc tố protein, gây ra hoặc làm trầm trọng thêm bệnh, nên việc ức chế hoạt động của độc tố vi khuẩn là một chiến lược chống độc lực đầy hứa hẹn. Trong bài đánh giá này, chúng tôi mô tả một số cách tiếp cận để ức chế các tương tác ban đầu của độc tố vi khuẩn với các thành phần màng tế bào chủ. Các cơ chế mà độc tố tương tác với các thành phần màng tế bào chủ đã được nghiên cứu kỹ lưỡng trong nhiều năm, dẫn đến việc xác định các mục tiêu điều trị, đã được khai thác trong công trình được mô tả ở đây. Chúng tôi xem xét các nỗ lực ức chế liên kết với thụ thể protein và các thành phần lipid màng thiết yếu, lắp ráp phức hợp và hình thành lỗ. Mặc dù chưa có phân tử nào trong số các phân tử này được chứng minh trong các thử nghiệm lâm sàng, nhưng các kết quả trong ống nghiệm và trong cơ thể sống được trình bày ở đây chứng minh tiềm năng của chúng như những phương pháp thay thế mới và/hoặc bổ sung cho các loại kháng sinh truyền thống."} +{"idx": 1900084, "en": "Background The industrial conversion of biomass to high-value biofuels and biochemical is mainly restricted by lignocellulose solubilization. Consolidated bio-saccharification (CBS) is considered a promising process for lignocellulose solubilization depending on whole-cell biocatalysts that simultaneously perform effective cellulase production and hydrolysis. However, it usually takes a long time to reach a high saccharification level using the current CBS biocatalyst and process. Results To promote the saccharification efficiency and reduce the cost, a Clostridium thermocellum recombinant strain ∆pyrF::KBm was constructed as a new CBS biocatalyst in this study. The key CBS factors, including the medium, inoculum size and cultivation, and substrate load, were investigated and optimized. The saccharification process was also stimulated by adding free hemicellulases, suggesting the need to further enhance hemicellulase activity of the whole-cell catalyst. Under the optimal conditions, the CBS process was shortened by 50% with pretreated wheat straw as the substrate. The sugar yield reached 0.795 g/g and the saccharification level was 89.3%. Conclusions This work provided a new biocatalyst and an optimized process of CBS and confirmed that CBS is a feasible strategy for cost-efficient solubilization of lignocellulose, which will greatly promote the industrial utilization of lignocellulosic biomass.", "gemini": "Bối cảnh Việc chuyển đổi sinh khối công nghiệp thành nhiên liệu sinh học và hóa chất sinh học giá trị cao chủ yếu bị hạn chế bởi quá trình hòa tan lignocellulose. Quá trình saccharification sinh học hợp nhất (CBS) được coi là một quá trình đầy hứa hẹn để hòa tan lignocellulose tùy thuộc vào các chất xúc tác sinh học toàn tế bào thực hiện đồng thời quá trình sản xuất cellulase và thủy phân hiệu quả. Tuy nhiên, thường mất nhiều thời gian để đạt được mức saccharification cao bằng cách sử dụng chất xúc tác sinh học CBS và quy trình hiện tại. Kết quả Để thúc đẩy hiệu quả saccharification và giảm chi phí, một chủng Clostridium thermocellum tái tổ hợp ∆pyrF::KBm đã được xây dựng như một chất xúc tác sinh học CBS mới trong nghiên cứu này. Các yếu tố CBS chính, bao gồm môi trường, kích thước và nuôi cấy inốc, và tải chất nền, đã được nghiên cứu và tối ưu hóa. Quá trình saccharification cũng được kích thích bằng cách thêm các hemicellulase miễn phí, cho thấy cần phải tăng cường thêm hoạt động của hemicellulase của chất xúc tác toàn tế bào. Trong điều kiện tối ưu, quá trình CBS được rút ngắn 50% với rơm lúa mì đã qua xử lý trước là chất nền. Năng suất đường đạt 0,795 g/g và mức saccharification là 89,3%. Kết luận Công trình này đã cung cấp một chất xúc tác sinh học mới và một quy trình CBS được tối ưu hóa và xác nhận rằng CBS là một chiến lược khả thi để hòa tan lignocellulose hiệu quả về chi phí, điều này sẽ thúc đẩy rất nhiều việc sử dụng sinh khối lignocellulose trong công nghiệp."} +{"idx": 1900085, "en": "Background The filamentous fungus Trichoderma reesei produces cellulase enzymes that are widely studied for lignocellulose bioconversion to biofuel. N,N-dimethylformamide (DMF) is a versatile organic solvent used in large quantities in industries. Results In this study, we serendipitously found that biologically relevant concentrations of extracellular DMF-induced cellulase production in the T. reesei hyper-cellulolytic mutant Rut-C30 and wild-type strain QM6a. Next, by transcriptome analysis, we determined that plc-e encoding phospholipase C was activated by DMF and revealed that cytosolic Ca2+ plays a vital role in the response of T. reesei to DMF. Using EGTA (a putative extracellular Ca2+ chelator) and LaCl3 (a plasma membrane Ca2+ channel blocker), we demonstrated that DMF induced a cytosolic Ca2+ burst via extracellular Ca2+ and Ca2+ channels in T. reesei, and that the cytosolic Ca2+ burst induced by DMF-mediated overexpression of cellulase through calcium signaling. Deletion of crz1 confirmed that calcium signaling plays a dominant role in DMF-induced cellulase production. Additionally, 0.5-2% DMF increases the permeability of T. reesei mycelia for cellulase release. Simultaneous supplementation with 1% DMF and 10 mM Mn2+ to T. reesei Rut-C30 increased cellulase activity approximately fourfold compared to that without treatment and was also more than that observed in response to either treatment alone. Conclusions Our results reveal that DMF-induced cellulase production via calcium signaling and permeabilization. Our results also provide insight into the role of calcium signaling in enzyme production for enhanced cellulase production and the development of novel inducers of cellulase.", "gemini": "Bối cảnh Nấm sợi Trichoderma reesei sản xuất các enzyme cellulase được nghiên cứu rộng rãi để chuyển đổi sinh khối lignocellulose thành nhiên liệu sinh học. N,N-dimethylformamide (DMF) là một dung môi hữu cơ đa năng được sử dụng với số lượng lớn trong các ngành công nghiệp. Kết quả Trong nghiên cứu này, chúng tôi tình cờ phát hiện ra rằng nồng độ DMF ngoại bào có liên quan đến sinh học đã gây ra sản xuất cellulase ở đột biến tăng tế bào T. reesei Rut-C30 và chủng QM6a kiểu hoang dã. Tiếp theo, bằng phân tích phiên mã, chúng tôi xác định rằng plc-e mã hóa phospholipase C đã được DMF kích hoạt và tiết lộ rằng Ca2+ tế bào chất đóng vai trò quan trọng trong phản ứng của T. reesei đối với DMF. Sử dụng EGTA (chất chelat Ca2+ ngoại bào giả định) và LaCl3 (chất chặn kênh Ca2+ màng tế bào), chúng tôi đã chứng minh rằng DMF gây ra sự bùng nổ Ca2+ tế bào chất thông qua Ca2+ ngoại bào và các kênh Ca2+ ở T. reesei, và sự bùng nổ Ca2+ tế bào chất do DMF gây ra sự biểu hiện quá mức của cellulase thông qua tín hiệu canxi. Việc xóa crz1 đã xác nhận rằng tín hiệu canxi đóng vai trò chủ đạo trong quá trình sản xuất cellulase do DMF gây ra. Ngoài ra, 0,5-2% DMF làm tăng tính thấm của sợi nấm T. reesei để giải phóng cellulase. Bổ sung đồng thời 1% DMF và 10 mM Mn2+ cho T. reesei Rut-C30 làm tăng hoạt động của cellulase khoảng bốn lần so với không xử lý và cũng cao hơn so với phản ứng với một trong hai phương pháp xử lý riêng lẻ. Kết luận Kết quả của chúng tôi cho thấy rằng sản xuất cellulase do DMF gây ra thông qua tín hiệu canxi và tính thấm. Kết quả của chúng tôi cũng cung cấp thông tin chi tiết về vai trò của tín hiệu canxi trong sản xuất enzyme để tăng cường sản xuất cellulase và phát triển các chất gây cảm ứng cellulase mới."} +{"idx": 1900086, "en": "Background Prader-Willi syndrome (PWS) and Angelman syndrome (AS) are clinically distinct neurodevelopmental disorders caused by absence of paternally or maternally expressed imprinted genes on chromosome 15q11.2-q13.3 region. Methods 3331 individuals was recruited from June 2013 to December 2016 under an institutional review board-approved protocol of informed consent. The methylation-specific PCR was employed as a first-tier screening test. The multiplex-fluorescent-labeled STR linkage analysis was carried out to define the underlying genetic mechanisms. The chromosomal microarray analysis was employed to identify chromosomal breakpoints in confirmed cases, and to detect other chromosomal abnormalities in undiagnosed cases. Genetic counseling and recurrence risk assessment were provided to families with affected individuals. Results The methylation-specific PCR identified 36 PWS suspected patients and 13 AS suspected patients. UBE3A sequence analysis identified another 1 patient with AS. The STR linkage analysis define the underlying genetic mechanisms. Thirty PWS patients were with paternal deletions on chromosome region 15q11-q13, 5 with isodisomic uniparental disomy and 1 with mixed segmental isodisomic/ heterodisomic uniparental disomy of maternal chromosome 15. Twelve AS patients were with maternal deletions, 1 with isodisomic uniparental disomy and 1 with UBE3A gene mutation. The chromosomal microarray analysis identified chromosomal breakpoints in confirmed cases, and detected chromosomal abnormalities in another 4 patients with clinically overlapped features but tested negative for PWS/AS. Genetic counseling was offered to all families with affected individuals. Conclusions Identifying the disorders at early age, establishing the molecular mechanisms, carrying out treatment intervention and close monitoring can significantly improve the prognosis of PWS/AS patients.", "gemini": "Bối cảnh Hội chứng Prader-Willi (PWS) và hội chứng Angelman (AS) là những rối loạn phát triển thần kinh riêng biệt về mặt lâm sàng do thiếu gen in hằn biểu hiện từ cha hoặc mẹ trên vùng nhiễm sắc thể 15q11.2-q13.3. Phương pháp 3331 cá nhân đã được tuyển dụng từ tháng 6 năm 2013 đến tháng 12 năm 2016 theo một giao thức được hội đồng đánh giá thể chế chấp thuận về sự đồng ý được thông báo. PCR đặc hiệu metyl hóa được sử dụng làm xét nghiệm sàng lọc cấp một. Phân tích liên kết STR gắn nhãn huỳnh quang đa kênh được thực hiện để xác định cơ chế di truyền cơ bản. Phân tích mảng nhiễm sắc thể được sử dụng để xác định điểm gãy nhiễm sắc thể ở các trường hợp được xác nhận và để phát hiện các bất thường nhiễm sắc thể khác ở các trường hợp chưa được chẩn đoán. Tư vấn di truyền và đánh giá rủi ro tái phát đã được cung cấp cho các gia đình có cá nhân bị ảnh hưởng. Kết quả PCR đặc hiệu metyl hóa đã xác định 36 bệnh nhân nghi ngờ mắc PWS và 13 bệnh nhân nghi ngờ mắc AS. Phân tích trình tự UBE3A xác định thêm 1 bệnh nhân mắc AS. Phân tích liên kết STR xác định cơ chế di truyền cơ bản. Ba mươi bệnh nhân PWS bị mất đoạn từ cha trên vùng nhiễm sắc thể 15q11-q13, 5 bị đồng hợp tử đơn bội đẳng đoạn và 1 bị đồng hợp tử đơn bội đẳng đoạn phân đoạn hỗn hợp/đơn bội dị đoạn của nhiễm sắc thể 15 của mẹ. Mười hai bệnh nhân AS bị mất đoạn từ mẹ, 1 bị đồng hợp tử đơn bội đẳng đoạn và 1 bị đột biến gen UBE3A. Phân tích mảng nhiễm sắc thể xác định điểm gãy nhiễm sắc thể ở các trường hợp được xác nhận và phát hiện bất thường nhiễm sắc thể ở 4 bệnh nhân khác có các đặc điểm lâm sàng chồng chéo nhưng xét nghiệm âm tính với PWS/AS. Tư vấn di truyền đã được cung cấp cho tất cả các gia đình có cá nhân bị ảnh hưởng. Kết luận Việc xác định các rối loạn ở độ tuổi sớm, thiết lập cơ chế phân tử, thực hiện can thiệp điều trị và theo dõi chặt chẽ có thể cải thiện đáng kể tiên lượng của bệnh nhân PWS/AS."} +{"idx": 1900087, "en": "Background Helicobacter pylori is a Gram-negative bacterium which mainly causes peptic ulcer disease in human, but is also the predominant cause of stomach cancer. It has been coevolving with human since 120,000 years and, according to Multi-locus sequence typing (MLST), H. pylori can be classified into seven major population types, namely, hpAfrica1, hpAfrica2, hpNEAfrica, hpEastAsia, hpAsia2, hpEurope and hpSahul. Helicobacter pylori harbours a large number of restriction-modification (R-M) systems. The methyltransferase (MTase) unit plays a significant role in gene regulation and also possibly modulates pathogenicity. The diversity in MTase can act as geomarkers to correlate strains with the phylogeographic origins. This paper describes the complete genome sequence and methylome of gastric pathogen H. pylori belonging to the population hpNEAfrica. Results In this paper, we present the complete genome sequence and the methylome profile of H. pylori hpNEAfrica strain HP14039, isolated from a patient who was born in Somalia and likely to be infected locally during early childhood prior to migration. The genome of HP14039 consists of 1,678,260 bp with 1574 coding genes and 38.7% GC content. The sequence analysis showed that this strain lacks the cag pathogenicity island. The vacA gene is of S2M2 type. We have also identified 15 methylation motifs, including WCANHNNNNTG and CTANNNNNNNTAYG that were not previously described. Conclusions We have described the complete genome of H. pylori strain HP14039. The information regarding phylo-geography, methylome and associated metadata would help scientific community to study more about hpNEAfrica population type.", "gemini": "Bối cảnh Helicobacter pylori là một loại vi khuẩn Gram âm chủ yếu gây bệnh loét dạ dày tá tràng ở người, nhưng cũng là nguyên nhân chính gây ung thư dạ dày. Nó đã cùng tiến hóa với con người từ 120.000 năm trước và theo phân loại trình tự đa gen (MLST), H. pylori có thể được chia thành bảy loại quần thể chính, cụ thể là hpAfrica1, hpAfrica2, hpNEAfrica, hpEastAsia, hpAsia2, hpEurope và hpSahul. Helicobacter pylori chứa một số lượng lớn các hệ thống hạn chế-sửa đổi (R-M). Đơn vị methyltransferase (MTase) đóng vai trò quan trọng trong điều hòa gen và cũng có thể điều chỉnh độc lực. Sự đa dạng trong MTase có thể hoạt động như các dấu địa lý để tương quan các chủng với nguồn gốc địa lý. Bài báo này mô tả trình tự toàn bộ bộ gen và methylome của tác nhân gây bệnh dạ dày H. pylori thuộc quần thể hpNEAfrica. Kết quả Trong bài báo này, chúng tôi trình bày trình tự toàn bộ bộ gen và cấu hình methylome của chủng H. pylori hpNEAfrica HP14039, được phân lập từ một bệnh nhân sinh ra ở Somalia và có khả năng bị nhiễm trùng tại địa phương trong thời thơ ấu trước khi di cư. Bộ gen của HP14039 bao gồm 1.678.260 bp với 1574 gen mã hóa và hàm lượng GC 38,7%. Phân tích trình tự cho thấy chủng này thiếu đảo độc lực cag. Gen vacA thuộc kiểu S2M2. Chúng tôi cũng đã xác định được 15 mô típ mety hóa, bao gồm WCANHNNNNTG và CTANNNNNNNTAYG chưa từng được mô tả trước đây. Kết luận Chúng tôi đã mô tả toàn bộ bộ gen của chủng H. pylori HP14039. Thông tin về địa lý phả hệ, methylome và siêu dữ liệu liên quan sẽ giúp cộng đồng khoa học nghiên cứu thêm về loại quần thể hpNEAfrica."} +{"idx": 1900088, "en": "Pneumothorax is a well-recognised complication of end-stage COPD, but the management is often complex and may be complicated by other sequelae of advanced respiratory disease including the requirement for NIV http://ow.ly/vkoJ30mB4nZ.", "gemini": "Tràn khí màng phổi là một biến chứng được công nhận của bệnh phổi tắc nghẽn mãn tính giai đoạn cuối, nhưng việc quản lý thường phức tạp và có thể phức tạp do các di chứng khác của bệnh hô hấp tiến triển bao gồm yêu cầu về NIV http://ow.ly/vkoJ30mB4nZ."} +{"idx": 1900089, "en": "Latin America is a fast-growing region that currently faces unique challenges in the treatment of all forms of diabetes mellitus. The burden of this disease will be even greater in the coming years due, in part, to the large proportion of young adults living in urban areas and engaging in unhealthy lifestyles. Unfortunately, the national health systems in Latin-American countries are unprepared and urgently need to reorganize their health care services to achieve diabetic therapeutic goals. Stem cell research is attracting increasing attention as a promising and fast-growing field in Latin America. As future healthcare systems will include the development of regenerative medicine through stem cell research, Latin America is urged to issue a call-to-action on stem cell research. Increased efforts are required in studies focused on stem cells for the treatment of diabetes. In this review, we aim to inform physicians, researchers, patients and funding sources about the advances in stem cell research for possible future applications in diabetes mellitus. Emerging studies are demonstrating the potential of stem cells for β cell differentiation and pancreatic regeneration. The major economic burden implicated in patients with diabetes complications suggests that stem cell research may relieve diabetic complications. Closer attention should be paid to stem cell research in the future as an alternative treatment for diabetes mellitus.", "gemini": "Mỹ Latinh là một khu vực phát triển nhanh hiện đang phải đối mặt với những thách thức riêng biệt trong việc điều trị tất cả các dạng bệnh đái tháo đường. Gánh nặng của căn bệnh này sẽ còn lớn hơn trong những năm tới, một phần là do tỷ lệ lớn thanh niên sống ở khu vực đô thị và có lối sống không lành mạnh. Thật không may, các hệ thống y tế quốc gia ở các nước Mỹ Latinh chưa sẵn sàng và cần khẩn trương tổ chức lại các dịch vụ chăm sóc sức khỏe của mình để đạt được các mục tiêu điều trị bệnh tiểu đường. Nghiên cứu tế bào gốc đang thu hút sự chú ý ngày càng tăng với tư cách là một lĩnh vực đầy hứa hẹn và phát triển nhanh ở Mỹ Latinh. Vì các hệ thống chăm sóc sức khỏe trong tương lai sẽ bao gồm sự phát triển của y học tái tạo thông qua nghiên cứu tế bào gốc, Mỹ Latinh được kêu gọi hành động về nghiên cứu tế bào gốc. Cần tăng cường nỗ lực trong các nghiên cứu tập trung vào tế bào gốc để điều trị bệnh tiểu đường. Trong bài đánh giá này, chúng tôi muốn thông tin cho các bác sĩ, nhà nghiên cứu, bệnh nhân và các nguồn tài trợ về những tiến bộ trong nghiên cứu tế bào gốc để ứng dụng trong tương lai có thể có trong bệnh đái tháo đường. Các nghiên cứu mới đang chứng minh tiềm năng của tế bào gốc đối với sự biệt hóa tế bào β và tái tạo tuyến tụy. Gánh nặng kinh tế chính liên quan đến bệnh nhân mắc các biến chứng của bệnh tiểu đường cho thấy rằng nghiên cứu tế bào gốc có thể làm giảm các biến chứng của bệnh tiểu đường. Nên chú ý nhiều hơn đến nghiên cứu tế bào gốc trong tương lai như một phương pháp điều trị thay thế cho bệnh đái tháo đường."} +{"idx": 1900090, "en": "Non-resolving pneumonia with a mass-like radiological appearance raises clinical suspicion of many causes, with malignancy the most prominent. Infectious causes should be investigated thoroughly as they carry a better prognosis. http://ow.ly/QEPL30mLIk4.", "gemini": "Viêm phổi không hồi phục với hình ảnh X quang giống khối u làm tăng nghi ngờ lâm sàng về nhiều nguyên nhân, trong đó ác tính là nguyên nhân nổi bật nhất. Các nguyên nhân do nhiễm trùng cần được điều tra kỹ lưỡng vì chúng có tiên lượng tốt hơn. http://ow.ly/QEPL30mLIk4."} +{"idx": 1900091, "en": "Doubling time, clinical prediction models of malignancy and positive bronchus sign are useful in stepwise evaluation of SPN to avoid thoracotomy. GeneXpert can be used as initial diagnostic test for tuberculosis and detection of rifampicin resistance. 
http://ow.ly/N37030mB8Fi.", "gemini": "Thời gian nhân đôi, mô hình dự đoán lâm sàng về tình trạng ác tính và dấu phế quản dương tính rất hữu ích trong việc đánh giá từng bước SPN để tránh thoracotomy. GeneXpert có thể được sử dụng làm xét nghiệm chẩn đoán ban đầu cho bệnh lao và phát hiện tình trạng kháng rifampicin. 
http://ow.ly/N37030mB8Fi."} +{"idx": 1900092, "en": "In Turkey, respiratory medicine is a challenging speciality that requires the hard work and dedication of physicians. Chest physicians closely follow the worldwide innovations that enlighten the future directions of the field. http://ow.ly/XMe430n2JRx.", "gemini": "Ở Thổ Nhĩ Kỳ, chuyên khoa hô hấp là một chuyên ngành đầy thử thách đòi hỏi sự chăm chỉ và tận tụy của các bác sĩ. Các bác sĩ chuyên khoa lồng ngực theo dõi chặt chẽ những đổi mới trên toàn thế giới giúp soi sáng các hướng đi trong tương lai của lĩnh vực này. http://ow.ly/XMe430n2JRx."} +{"idx": 1900093, "en": "Here, we present the draft genome sequence of Apiotrichum porosum DSM 27194 generated on PacBio platform. Characterization of this oleaginous yeast originally collected from the grassland in Karlsruhe Germany, revealed potential for its utilization as a source of single cell oil (SCO) and gluconic acid (GA). The availability of the genome sequence provides a valuable resource for the elucidation of the genetic processes determining SCO and GA biosynthesis.", "gemini": "Ở đây, chúng tôi trình bày trình tự bản nháp bộ gen của Apiotrichum porosum DSM 27194 được tạo ra trên nền tảng PacBio. Đặc điểm của loại nấm men chứa nhiều dầu này ban đầu được thu thập từ đồng cỏ ở Karlsruhe, Đức, cho thấy tiềm năng sử dụng làm nguồn dầu tế bào đơn (SCO) và axit gluconic (GA). Việc sử dụng trình tự bộ gen cung cấp một nguồn tài nguyên có giá trị để làm sáng tỏ các quá trình di truyền xác định quá trình sinh tổng hợp SCO và GA."} +{"idx": 1900094, "en": "A summary of the ERS International Congress 2018 from the best-abstract awardees for each ERS Assembly and their views on the evolving field of research of their respective Assemblies http://ow.ly/c0eq30ntKuw.", "gemini": "Tóm tắt của Đại hội Quốc tế ERS 2018 từ những người được trao giải tóm tắt hay nhất cho mỗi Hội đồng ERS và quan điểm của họ về lĩnh vực nghiên cứu đang phát triển của Hội đồng tương ứng http://ow.ly/c0eq30ntKuw."} +{"idx": 1900095, "en": "Bacillus subtilis is a rod shaped, gram positive, spore producing bacterium. They are the normal flora of gastrointestinal tract of humans and it is the best characterized model organism for endospore formation. It has the ability to withstand environmental stress, and synthesize beneficial compounds, therefore, it is recognized as a high-quality probiotic supplement. To ensure the probiotic safety and the efficiency, we report the whole genome sequence (WGS) of Bacillus subtilis UBBS-14 strain. The draft genome sequence of Bacillus subtilis UBBS-14 consists of 4,048,984 bp and 4,017 genes, respectively. Bacillus subtilis UBBS-14 does not carry any antibiotic resistant genes containing plasmid, nor it contains any harmful putative virulence factors coding genes, therefore, it confirms the probiotic safety of the respective strain at genome level.", "gemini": "Bacillus subtilis là một loại vi khuẩn hình que, gram dương, tạo bào tử. Chúng là hệ vi sinh vật bình thường của đường tiêu hóa ở người và là sinh vật mô hình được đặc trưng tốt nhất để hình thành nội bào tử. Nó có khả năng chịu được căng thẳng môi trường và tổng hợp các hợp chất có lợi, do đó, nó được công nhận là một chất bổ sung probiotic chất lượng cao. Để đảm bảo an toàn và hiệu quả của probiotic, chúng tôi báo cáo trình tự toàn bộ bộ gen (WGS) của chủng Bacillus subtilis UBBS-14. Trình tự bản nháp bộ gen của Bacillus subtilis UBBS-14 bao gồm 4.048.984 bp và 4.017 gen. Bacillus subtilis UBBS-14 không mang bất kỳ gen kháng kháng sinh nào chứa plasmid, cũng không chứa bất kỳ gen mã hóa yếu tố độc lực có hại nào, do đó, nó xác nhận tính an toàn của probiotic của chủng tương ứng ở cấp độ bộ gen."} +{"idx": 1900096, "en": "Arthrobacter sp. strain MN05-02 is a UV-resistant bacterium isolated from a manganese deposit in the Sonoran Desert, Arizona, USA. The LD10 of this strain is 123 Jm-2, which is twice that of Escherichia coli, and therefore can be a useful resource for comparative study of UV resistance and the role of manganese on this phenotype. Its complete genome is comprised of a chromosome of 3,488,433 bp and a plasmid of 154,991 bp. The chromosome contains 3,430 putative genes, including 3,366 protein coding genes, 52 tRNA and 12 rRNA genes. Carotenoid biosynthesis operon structure coded within the genome mirrors the characteristic orange-red pigment this bacterium produces, which presumably partly contribute to its UV resistance.", "gemini": "Arthrobacter sp. chủng MN05-02 là một loại vi khuẩn chịu tia cực tím được phân lập từ một mỏ mangan ở Sa mạc Sonoran, Arizona, Hoa Kỳ. LD10 của chủng này là 123 Jm-2, gấp đôi so với Escherichia coli, do đó có thể là một nguồn hữu ích để nghiên cứu so sánh về khả năng chống tia cực tím và vai trò của mangan đối với kiểu hình này. Toàn bộ bộ gen của nó bao gồm một nhiễm sắc thể dài 3.488.433 bp và một plasmid dài 154.991 bp. Nhiễm sắc thể chứa 3.430 gen giả định, bao gồm 3.366 gen mã hóa protein, 52 gen tRNA và 12 gen rRNA. Cấu trúc operon sinh tổng hợp carotenoid được mã hóa trong bộ gen phản ánh sắc tố đỏ cam đặc trưng mà loại vi khuẩn này tạo ra, có lẽ đóng góp một phần vào khả năng chống tia cực tím của nó."} +{"idx": 1900097, "en": "Are touchscreen devices a public health risk for the transmission of pathogenic bacteria, especially those that are resistant to antibiotics? To investigate this, we embarked on a project aimed at isolating and identifying bacteria that are resistant to antibiotics from the screens of smartphones. Touchscreen devices have become ubiquitous in society, and it is important to evaluate the potential risks they pose towards public health, especially as it pertains to the harboring and transmission of pathogenic bacteria that are resistant to antibiotics. Sixteen bacteria were initially isolated of which five were unique (four Staphylococcus species and one Micrococcus species). The genomes of the five unique isolates were subsequently sequenced and annotated. The genomes were analyzed using in silico tools to predict the synthesis of antibiotics and secondary metabolites using the antibiotics and Secondary Metabolite Analysis SHell (antiSMASH) tool in addition to the presence of gene clusters that denote resistance to antibiotics using the Resistance Gene Identifier (RGI) tool. In vivo analysis was also done to assess resistance/susceptibility to four antibiotics that are commonly used in a research laboratory setting. The data presented in this manuscript is the result of a semester-long inquiry based laboratory exercise in the genomics course (BIOL340) in the Thomas H. Gosnell School of Life Sciences/College of Science at the Rochester Institute of Technology.", "gemini": "Liệu các thiết bị màn hình cảm ứng có phải là mối nguy hiểm đối với sức khỏe cộng đồng do lây truyền vi khuẩn gây bệnh, đặc biệt là những vi khuẩn kháng thuốc kháng sinh? Để tìm hiểu vấn đề này, chúng tôi đã bắt tay vào một dự án nhằm phân lập và xác định vi khuẩn kháng thuốc kháng sinh từ màn hình điện thoại thông minh. Các thiết bị màn hình cảm ứng đã trở nên phổ biến trong xã hội, và điều quan trọng là phải đánh giá những rủi ro tiềm ẩn mà chúng gây ra đối với sức khỏe cộng đồng, đặc biệt là liên quan đến việc chứa và truyền vi khuẩn gây bệnh kháng thuốc kháng sinh. Ban đầu, mười sáu loại vi khuẩn đã được phân lập, trong đó có năm loại độc đáo (bốn loài Staphylococcus và một loài Micrococcus). Bộ gen của năm phân lập độc đáo sau đó đã được giải trình tự và chú thích. Các bộ gen đã được phân tích bằng các công cụ in silico để dự đoán quá trình tổng hợp kháng sinh và các chất chuyển hóa thứ cấp bằng cách sử dụng công cụ Phân tích vỏ kháng sinh và chất chuyển hóa thứ cấp (antiSMASH) ngoài sự hiện diện của các cụm gen biểu thị khả năng kháng thuốc kháng sinh bằng cách sử dụng công cụ Xác định gen kháng (RGI). Phân tích in vivo cũng được thực hiện để đánh giá khả năng kháng/nhạy cảm với bốn loại kháng sinh thường được sử dụng trong môi trường phòng thí nghiệm nghiên cứu. Dữ liệu được trình bày trong bản thảo này là kết quả của một bài tập trong phòng thí nghiệm dựa trên quá trình tìm hiểu kéo dài một học kỳ trong khóa học về hệ gen (BIOL340) tại Trường Khoa học Đời sống/Cao đẳng Khoa học Thomas H. Gosnell thuộc Học viện Công nghệ Rochester."} +{"idx": 1900098, "en": "Background Higher physical activity is associated with lower chronic disease risk among older adults. However, less is known about the optimal balance between daily physical activity and sedentary time and their correlates among older adults. We described objectively measured physical activity patterns using 7 day hip-accelerometry and assessed its correlates in a large cross-sectional sample of the Longitudinal Aging Study Amsterdam, a population-based cohort of older Dutch adults. In addition, we examined different combined profiles of sedentary time and physical activity across strata of sex, age, education and BMI groups. Results Mean age was 71 (SD 8) years and 51% (n = 615) were women. The majority of wear time was spent sedentary (65%) followed by light (33%), and MVPA (2%). Higher age and higher BMI were related to more time spent sedentary, while female sex and lower education were related lower sedentary time. The combination of high sedentary time (≥65.4% of waking time) and low physical activity (< 9.1% of waking time) was significantly associated with higher age, higher BMI, and slower walking speed compared to the combination of low sedentary time and high physical activity P < 0.001. Conclusions Dutch older adults spend on average 65% of their waking time sedentary. Older adults' sedentary time differs by age, sex, education and BMI groups. The combination of high sedentary time and low physical was associated with higher age, higher BMI, and slower walking speed compared to the combination of low sedentary time and high MVPA. This suggests that increasing light activity might be an effective and feasible strategy in older persons to reduce sedentary time. Future studies should assess whether low- sedentary and high-light physical activity are associated with improved long-term health outcomes (also independent of MVPA).", "gemini": "Bối cảnh Hoạt động thể chất cao hơn có liên quan đến nguy cơ mắc bệnh mãn tính thấp hơn ở người lớn tuổi. Tuy nhiên, người ta biết ít hơn về sự cân bằng tối ưu giữa hoạt động thể chất hàng ngày và thời gian ít vận động cũng như mối tương quan của chúng ở người lớn tuổi. Chúng tôi đã mô tả các kiểu hoạt động thể chất được đo khách quan bằng máy đo gia tốc hông trong 7 ngày và đánh giá mối tương quan của chúng trong một mẫu cắt ngang lớn của Nghiên cứu lão hóa dọc theo thời gian Amsterdam, một nhóm người lớn tuổi Hà Lan theo dõi dựa trên dân số. Ngoài ra, chúng tôi đã kiểm tra các cấu hình kết hợp khác nhau của thời gian ít vận động và hoạt động thể chất trên các tầng lớp giới tính, độ tuổi, trình độ học vấn và nhóm BMI. Kết quả Độ tuổi trung bình là 71 (SD 8) và 51% (n = 615) là nữ. Phần lớn thời gian đeo là ít vận động (65%) tiếp theo là nhẹ (33%) và MVPA (2%). Tuổi cao hơn và BMI cao hơn có liên quan đến thời gian ít vận động nhiều hơn, trong khi giới tính nữ và trình độ học vấn thấp hơn có liên quan đến thời gian ít vận động thấp hơn. Sự kết hợp giữa thời gian ít vận động cao (≥65,4% thời gian thức) và hoạt động thể chất thấp (<9,1% thời gian thức) có liên quan đáng kể đến tuổi cao hơn, BMI cao hơn và tốc độ đi chậm hơn so với sự kết hợp giữa thời gian ít vận động thấp và hoạt động thể chất cao P <0,001. Kết luận Người lớn tuổi Hà Lan dành trung bình 65% thời gian thức của mình để ít vận động. Thời gian ít vận động của người lớn tuổi khác nhau tùy theo độ tuổi, giới tính, trình độ học vấn và nhóm BMI. Sự kết hợp giữa thời gian ít vận động cao và hoạt động thể chất thấp có liên quan đến tuổi cao hơn, BMI cao hơn và tốc độ đi chậm hơn so với sự kết hợp giữa thời gian ít vận động thấp và MVPA cao. Điều này cho thấy rằng việc tăng cường hoạt động nhẹ có thể là một chiến lược hiệu quả và khả thi ở người lớn tuổi để giảm thời gian ít vận động. Các nghiên cứu trong tương lai nên đánh giá xem hoạt động thể chất ít vận động và nhiều ánh sáng có liên quan đến kết quả sức khỏe lâu dài được cải thiện hay không (cũng không phụ thuộc vào MVPA)."} +{"idx": 1900099, "en": "Objective The purpose of this study was to draw a global portrait of the current knowledge and interest regarding nutrigenetics in a population of French Canadians from the province of Quebec (Canada). Methods A total of 2238 residents from the province of Quebec, Canada, were recruited via social networks and from the Laval University employee/student lists to participate in a 37-question online survey on nutrigenetics. Results Most participants were not familiar with the term \"nutrigenetics\" (82.7%). Participants with good genetic literacy (26.8%) were less interested in nutrigenetic testing (p < 0.0001). The vast majority of participants (90.7%) reported to be willing to follow a personalised diet based on nutrigenetic testing, especially if they came to know themselves as carriers of a polymorphism increasing the risk of certain diseases. Participants had a higher interest in testing related to metabolic response to macronutrients (types of sugars, fats and proteins) than to micronutrients or other nutrients related to food intolerance. Conclusions The attitude of French Canadians about nutrigenetics is very consistent with the results from other surveys published in the literature. Although few individuals are familiar with nutrigenetics, the public's attitude towards nutrigenetics is globally favourable.", "gemini": "Mục tiêu Mục đích của nghiên cứu này là vẽ nên bức chân dung toàn cầu về kiến thức và sở thích hiện tại liên quan đến dinh dưỡng gen trong quần thể người Canada gốc Pháp từ tỉnh Quebec (Canada). Phương pháp Tổng cộng 2238 cư dân từ tỉnh Quebec, Canada, đã được tuyển dụng thông qua mạng xã hội và từ danh sách nhân viên/sinh viên của Đại học Laval để tham gia vào cuộc khảo sát trực tuyến gồm 37 câu hỏi về dinh dưỡng gen. Kết quả Hầu hết những người tham gia không quen thuộc với thuật ngữ \"dinh dưỡng gen\" (82,7%). Những người tham gia có hiểu biết tốt về di truyền (26,8%) ít quan tâm hơn đến xét nghiệm dinh dưỡng gen (p < 0,0001). Phần lớn những người tham gia (90,7%) cho biết sẵn sàng tuân theo chế độ ăn uống được cá nhân hóa dựa trên xét nghiệm dinh dưỡng gen, đặc biệt nếu họ biết mình là người mang đa hình làm tăng nguy cơ mắc một số bệnh nhất định. Những người tham gia quan tâm nhiều hơn đến việc xét nghiệm liên quan đến phản ứng trao đổi chất đối với các chất đa lượng (các loại đường, chất béo và protein) hơn là các chất vi lượng hoặc các chất dinh dưỡng khác liên quan đến chứng không dung nạp thực phẩm. Kết luận Thái độ của người Canada gốc Pháp về dinh dưỡng gen rất phù hợp với kết quả từ các cuộc khảo sát khác được công bố trong tài liệu. Mặc dù rất ít cá nhân quen thuộc với dinh dưỡng gen, nhưng thái độ của công chúng đối với dinh dưỡng gen nhìn chung là thuận lợi."} +{"idx": 1900100, "en": "Background Hepatic insulin resistance (IR) plays a crucial role in the development of many metabolic diseases, such as type 2 diabetes. MicroRNAs (miRNAs) are involved in the pathogenesis of IR and related diseases; however, studies of miRNAs in hepatic IR are limited. Method In this study, we adopted a high-throughput sequencing approach to construct small RNA libraries in the livers of normal mice and high-fat diet-induced hepatic IR mice. Results Through analysis of data, 107 known and 56 novel miRNAs were identified as differentially expressed miRNAs between the two groups. Additionally, bioinformatics methods were used to predict targets of the differentially expressed miRNAs and to explore the potential downstream Gene Ontology categories and Kyoto Encyclopedia of Genes and Genomes pathways. Meanwhile, some differentially expressed miRNAs (miR-34a-5p, miR-149-5p, miR-335-3p, miR-10b-5p, miR-1a-3p, miR-411-5p, and miR-592-5p) were validated by quantitative-time PCR, and their potential target genes related to IR or glycolipid metabolism were also predicted and presented in this study. Conclusion Taken together, our results defined miRNA expression signature that may lead to hepatic IR in mice, and the findings provided a foundation for future studies to further explore the effects and underlying mechanisms of the miRNAs and their target genes in the pathogenesis of hepatic IR and related diseases.", "gemini": "Bối cảnh Kháng insulin ở gan (IR) đóng vai trò quan trọng trong sự phát triển của nhiều bệnh chuyển hóa, chẳng hạn như bệnh tiểu đường loại 2. MicroRNA (miRNA) tham gia vào quá trình sinh bệnh của IR và các bệnh liên quan; tuy nhiên, các nghiên cứu về miRNA trong IR ở gan còn hạn chế. Phương pháp Trong nghiên cứu này, chúng tôi đã áp dụng phương pháp giải trình tự thông lượng cao để xây dựng các thư viện RNA nhỏ trong gan của chuột bình thường và chuột IR ở gan do chế độ ăn nhiều chất béo gây ra. Kết quả Thông qua phân tích dữ liệu, 107 miRNA đã biết và 56 miRNA mới được xác định là miRNA biểu hiện khác biệt giữa hai nhóm. Ngoài ra, các phương pháp tin sinh học đã được sử dụng để dự đoán các mục tiêu của miRNA biểu hiện khác biệt và khám phá các danh mục Gene Ontology tiềm năng và các con đường Bách khoa toàn thư về gen và bộ gen của Kyoto. Trong khi đó, một số miRNA biểu hiện khác biệt (miR-34a-5p, miR-149-5p, miR-335-3p, miR-10b-5p, miR-1a-3p, miR-411-5p và miR-592-5p) đã được xác nhận bằng định lượng thời gian. PCR và các gen mục tiêu tiềm năng của chúng liên quan đến IR hoặc chuyển hóa glycolipid cũng được dự đoán và trình bày trong nghiên cứu này. Kết luận Kết hợp lại, kết quả của chúng tôi đã xác định được đặc điểm biểu hiện miRNA có thể dẫn đến IR ở gan ở chuột và những phát hiện này cung cấp nền tảng cho các nghiên cứu trong tương lai để khám phá sâu hơn về tác dụng và cơ chế tiềm ẩn của miRNA và gen mục tiêu của chúng trong quá trình sinh bệnh của IR ở gan và các bệnh liên quan."} +{"idx": 1900101, "en": "The effects of exhaust emissions on public welfare have prompted the US Environmental Protection Agency to take various actions toward understanding, modeling, and reducing air pollution from vehicles. This study was performed to better understand exhaust emissions of heavy-duty diesel-powered tractor-trailer trucks that operate in drayage service, which involves the moving of shipping containers to or from port terminals. The study involved the use of portable emissions measurement systems (PEMS) to measure both gaseous and particulate matter (PM) mass emission rates and record various vehicle and engine parameters from the test trucks as they performed their normal drayage service. These measurements were supplemented with port terminal gate entry/exit logs for all drayage trucks entering the two Port of Houston Authority container terminals. The datasets were combined to analyze model year characteristics of drayage trucks over time, evaluate port visit frequencies and durations, assess geographic distributions of trucks that perform port service, and estimate the pollutant emissions related to drayage operations. When compared to certification results, measured pollutant emissions generally exceeded certification standards in terms of nitrogen oxides and PM, although this difference may be partly because these vehicles were not tested on certification cycles but rather actual in-use operation. The findings of this work indicate that less than 3.5 percent of gaseous drayage truck emissions are released during operation within port terminals, as drayage trucks operate at higher engine loads during their extensive travels throughout the region around the port.", "gemini": "Tác động của khí thải từ xe cộ đối với phúc lợi công cộng đã thúc đẩy Cơ quan Bảo vệ Môi trường Hoa Kỳ thực hiện nhiều hành động hướng tới việc hiểu biết, lập mô hình và giảm ô nhiễm không khí từ xe cộ. Nghiên cứu này được thực hiện để hiểu rõ hơn về khí thải từ xe đầu kéo chạy bằng động cơ diesel hạng nặng hoạt động trong dịch vụ vận chuyển đường bộ, bao gồm việc di chuyển các container vận chuyển đến hoặc đi từ các nhà ga cảng. Nghiên cứu liên quan đến việc sử dụng các hệ thống đo lường khí thải di động (PEMS) để đo cả tốc độ phát thải khối lượng vật chất dạng hạt (PM) và khí và ghi lại các thông số khác nhau của xe và động cơ từ các xe tải thử nghiệm khi chúng thực hiện dịch vụ vận chuyển đường bộ bình thường. Những phép đo này được bổ sung bằng nhật ký ra vào cổng nhà ga cảng đối với tất cả các xe tải vận chuyển đường bộ vào hai nhà ga container của Cảng Houston. Các tập dữ liệu được kết hợp để phân tích các đặc điểm về năm sản xuất của xe tải vận chuyển đường bộ theo thời gian, đánh giá tần suất và thời gian ghé cảng, đánh giá phân bố địa lý của xe tải thực hiện dịch vụ cảng và ước tính lượng khí thải ô nhiễm liên quan đến hoạt động vận chuyển đường bộ. Khi so sánh với kết quả chứng nhận, khí thải ô nhiễm được đo lường thường vượt quá các tiêu chuẩn chứng nhận về nitơ oxit và PM, mặc dù sự khác biệt này có thể một phần là do những chiếc xe này không được thử nghiệm theo các chu kỳ chứng nhận mà là hoạt động thực tế. Những phát hiện của công trình này chỉ ra rằng ít hơn 3,5 phần trăm lượng khí thải từ xe tải vận chuyển đường bộ dạng khí được thải ra trong quá trình hoạt động bên trong các nhà ga cảng, vì xe tải vận chuyển đường bộ hoạt động ở tải trọng động cơ cao hơn trong suốt quá trình di chuyển rộng rãi của chúng trong khu vực xung quanh cảng."} +{"idx": 1900102, "en": "Non-alcoholic fatty liver disease (NAFLD) is increasingly recognized as a significant liver disease, and it covers the disease spectrum from simple steatosis with a risk of development of non-alcoholic steatohepatitis (NASH) to fibrosis, subsequent cirrhosis, end-stage liver failure, and liver cancer with a potential need for liver transplantation. NAFLD and NASH are closely related to obesity, metabolic syndrome, and type 2 diabetes (T2D). The role of gut hormones, especially glucagon-like peptide 1 (GLP-1), is important in NAFLD. Bariatric surgery has the potential for inducing great weight loss and may improve the symptoms of metabolic syndrome and T2D. Recent data demonstrated significant effects of bariatric surgery on GLP-1 and other gut hormones and important lipid metabolic and inflammatory abnormalities in the pathophysiology of NAFLD. Therefore, bariatric surgery may reverse the pathological liver changes in NAFLD and NASH patients. In the present review, we describe NAFLD and NASH pathophysiology and the primary effects of bariatric surgery on metabolic pathways. We performed a systematic review of the beneficial and harmful effects and focused on changes in liver disease severity in NAFLD and NASH patients. The specific focus was liver histopathology as assessed by the invasive liver biopsy. Additionally, we reviewed several non-invasive methods used for the assessment of liver disease severity following bariatric surgery.", "gemini": "Bệnh gan nhiễm mỡ không do rượu (NAFLD) ngày càng được công nhận là một bệnh gan đáng kể và nó bao gồm phổ bệnh từ gan nhiễm mỡ đơn giản có nguy cơ phát triển thành viêm gan nhiễm mỡ không do rượu (NASH) đến xơ gan, xơ gan tiếp theo, suy gan giai đoạn cuối và ung thư gan có khả năng cần ghép gan. NAFLD và NASH có liên quan chặt chẽ với béo phì, hội chứng chuyển hóa và bệnh tiểu đường loại 2 (T2D). Vai trò của các hormone đường ruột, đặc biệt là glucagon-like peptide 1 (GLP-1), rất quan trọng trong NAFLD. Phẫu thuật cắt dạ dày có khả năng gây giảm cân đáng kể và có thể cải thiện các triệu chứng của hội chứng chuyển hóa và T2D. Dữ liệu gần đây chứng minh tác dụng đáng kể của phẫu thuật cắt dạ dày đối với GLP-1 và các hormone đường ruột khác và các bất thường quan trọng về chuyển hóa lipid và viêm trong bệnh sinh của NAFLD. Do đó, phẫu thuật cắt dạ dày có thể đảo ngược các thay đổi bệnh lý ở gan ở những bệnh nhân mắc NAFLD và NASH. Trong bài tổng quan hiện tại, chúng tôi mô tả bệnh sinh NAFLD và NASH và các tác động chính của phẫu thuật cắt dạ dày đối với các con đường chuyển hóa. Chúng tôi đã thực hiện một đánh giá có hệ thống về các tác động có lợi và có hại và tập trung vào những thay đổi về mức độ nghiêm trọng của bệnh gan ở những bệnh nhân mắc NAFLD và NASH. Trọng tâm cụ thể là bệnh lý mô học gan được đánh giá bằng sinh thiết gan xâm lấn. Ngoài ra, chúng tôi đã xem xét một số phương pháp không xâm lấn được sử dụng để đánh giá mức độ nghiêm trọng của bệnh gan sau phẫu thuật cắt dạ dày."} +{"idx": 1900103, "en": "Hepatic encephalopathy (HE) is a major complication that is closely related to the progression of end-stage liver disease. Metabolic changes in advanced liver failure can promote cognition impairment, attention deficits and motor dysfunction that may result in coma and death. HE can be subdivided according to the type of hepatic injury, namely, type A, which results from acute liver failure, type B, which is associated with a portosystemic shunting without intrinsic liver disease, and type C, which is due to chronic liver disease. Several studies have investigated the pathogenesis of the disease, and most of the mechanisms have been explored using animal models. This article aimed to review the use of preclinical models to investigate HE. The most used animal species are rats and mice. Experimental models of type A HE include surgical procedures and the administration of hepatotoxic medications, whereas models of types B and C HE are generally surgically induced lesions in liver tissue, which evolve to hepatic cirrhosis. Preclinical models have allowed the comprehension of the pathways related to HE.", "gemini": "Bệnh não gan (HE) là một biến chứng chính có liên quan chặt chẽ đến sự tiến triển của bệnh gan giai đoạn cuối. Những thay đổi về chuyển hóa trong suy gan tiến triển có thể thúc đẩy suy giảm nhận thức, thiếu chú ý và rối loạn vận động có thể dẫn đến hôn mê và tử vong. HE có thể được phân loại theo loại tổn thương gan, cụ thể là type A, là kết quả của suy gan cấp, type B, liên quan đến sự phân lưu cửa chủ mà không có bệnh gan nội tại, và type C, là do bệnh gan mãn tính. Một số nghiên cứu đã điều tra về bệnh sinh của bệnh và hầu hết các cơ chế đã được khám phá bằng cách sử dụng các mô hình động vật. Bài báo này nhằm mục đích xem xét việc sử dụng các mô hình tiền lâm sàng để điều tra HE. Các loài động vật được sử dụng nhiều nhất là chuột và chuột nhắt. Các mô hình thực nghiệm của HE type A bao gồm các thủ thuật phẫu thuật và sử dụng thuốc gây độc gan, trong khi các mô hình HE type B và C thường là các tổn thương được gây ra do phẫu thuật ở mô gan, tiến triển thành xơ gan. Các mô hình tiền lâm sàng đã cho phép hiểu được các con đường liên quan đến HE."} +{"idx": 1900104, "en": "Liver metastasis is the commonest form of distant metastasis in colorectal cancer. Selection criteria for surgery and liver-directed therapies have recently been extended. However, resectability remains poorly defined. Tumour biology is increasingly recognized as an important prognostic factor; hence molecular profiling has a growing role in risk stratification and management planning. Surgical resection is the only treatment modality for curative intent. The most appropriate surgical approach is yet to be established. The primary cancer and the hepatic metastasis can be removed simultaneously or in a two-step approach; these two strategies have comparable long-term outcomes. For patients with a limited future liver remnant, portal vein embolization, combined ablation and resection, and associating liver partition and portal vein ligation for staged hepatectomy have been advocated, and each has their pros and cons. The role of neoadjuvant and adjuvant chemotherapy is still debated. Targeted biological agents and loco-regional therapies (thermal ablation, intra-arterial chemo- or radio-embolization, and stereotactic radiotherapy) further improve the already favourable results. The recent debate about offering liver transplantation to highly selected patients needs validation from large clinical trials. Evidence-based protocols are missing, and therefore optimal management of hepatic metastasis should be personalized and determined by a multi-disciplinary team.", "gemini": "Di căn gan là dạng di căn xa phổ biến nhất ở ung thư đại trực tràng. Các tiêu chí lựa chọn phẫu thuật và các liệu pháp điều trị hướng đến gan gần đây đã được mở rộng. Tuy nhiên, khả năng cắt bỏ vẫn chưa được xác định rõ. Sinh học khối u ngày càng được công nhận là một yếu tố tiên lượng quan trọng; do đó, hồ sơ phân tử có vai trò ngày càng tăng trong phân tầng rủi ro và lập kế hoạch quản lý. Cắt bỏ bằng phẫu thuật là phương thức điều trị duy nhất có mục đích chữa khỏi. Phương pháp phẫu thuật thích hợp nhất vẫn chưa được xác định. Có thể cắt bỏ ung thư nguyên phát và di căn gan đồng thời hoặc theo phương pháp hai bước; hai chiến lược này có kết quả dài hạn tương đương nhau. Đối với những bệnh nhân có phần gan còn lại trong tương lai hạn chế, đã có ý kiến ủng hộ thuyên tắc tĩnh mạch cửa, kết hợp cắt bỏ và cắt bỏ, và kết hợp phân chia gan và thắt tĩnh mạch cửa để cắt gan theo giai đoạn và mỗi phương pháp đều có ưu và nhược điểm riêng. Vai trò của hóa trị tân bổ trợ và bổ trợ vẫn còn gây tranh cãi. Các tác nhân sinh học có mục tiêu và các liệu pháp tại chỗ (phá hủy nhiệt, hóa trị hoặc thuyên tắc động mạch và xạ trị lập thể) giúp cải thiện thêm các kết quả vốn đã thuận lợi. Cuộc tranh luận gần đây về việc cung cấp ghép gan cho những bệnh nhân được lựa chọn kỹ lưỡng cần được xác nhận từ các thử nghiệm lâm sàng lớn. Các giao thức dựa trên bằng chứng vẫn còn thiếu và do đó, việc quản lý tối ưu di căn gan nên được cá nhân hóa và xác định bởi một nhóm đa ngành."} +{"idx": 1900105, "en": "BACKGROUND Major hepatectomies are routinely performed because they are often the only curative treatment for metastatic liver disease. There has been a trend to concentrate major hepatectomies in referral hospitals that perform these operations at high volumes. These high volume referral centers are usually located in developed countries, but many patients in developing nations are not able to access these centers because of financial limitations, lack of social support and/or travel restrictions. Therefore, local hospitals are often the only options many of these patients have for surgical treatment of metastatic liver disease. This is the situation in many Caribbean countries. AIM To determine the clinical outcomes after major liver resections in a low-resource hepatobiliary center in the Caribbean. METHODS We prospectively studied all patients who underwent major liver resections over five years. The following data were extracted: patient demographics, diagnoses, ECOG status, operation performed, post-operative morbidity and mortality. Statistical analyses were performed using SPSS ver 16.0. RESULTS There were 69 major liver resections performed by two teams at a mean case volume of 13.8 major resections/year. Sixty-nine major hepatic resections were performed for: colorectal liver metastases 40 (58%), non-colorectal metastases 9 (13%), hepatocellular carcinoma 8 (11.6%), ruptured adenomas 4 (5.8%), hilar cholangiocarcinomas 4 (5.8%), hemangiomata 2 (2.9%), trauma 1 (1.5%) and hepatoblastoma 1 (1.5%). Twenty-one patients had at least one complication, for an overall morbidity rate of 30.4%. There were minor complications in 17 (24.6%) patients, major complications in 11 (15.9%) patients and 4 (5.8%) deaths. CONCLUSION There are unique geographic, political and financial limitations to healthcare delivery in the Caribbean. Nevertheless, clinical outcomes are acceptable in the established, low-volume hepatobiliary centers in the Eastern Caribbean.", "gemini": "BỐI CẢNH Các ca cắt gan lớn thường được thực hiện thường quy vì chúng thường là phương pháp điều trị duy nhất có thể chữa khỏi bệnh di căn gan. Có một xu hướng tập trung các ca cắt gan lớn tại các bệnh viện chuyên khoa thực hiện những ca phẫu thuật này với khối lượng lớn. Những trung tâm chuyên khoa khối lượng lớn này thường nằm ở các nước phát triển, nhưng nhiều bệnh nhân ở các nước đang phát triển không thể tiếp cận được những trung tâm này vì hạn chế về tài chính, thiếu sự hỗ trợ xã hội và/hoặc hạn chế đi lại. Do đó, các bệnh viện địa phương thường là lựa chọn duy nhất mà nhiều bệnh nhân này có thể lựa chọn để điều trị phẫu thuật bệnh di căn gan. Đây là tình hình ở nhiều quốc gia Caribe. MỤC ĐÍCH Xác định kết quả lâm sàng sau các ca cắt gan lớn tại một trung tâm gan mật có nguồn lực thấp ở Caribe. PHƯƠNG PHÁP Chúng tôi đã nghiên cứu triển vọng tất cả các bệnh nhân đã trải qua các ca cắt gan lớn trong năm năm. Các dữ liệu sau đây đã được trích xuất: thông tin nhân khẩu học của bệnh nhân, chẩn đoán, tình trạng ECOG, ca phẫu thuật được thực hiện, tỷ lệ mắc bệnh và tử vong sau phẫu thuật. Các phân tích thống kê đã được thực hiện bằng SPSS ver 16.0. KẾT QUẢ Đã có 69 ca cắt gan lớn được thực hiện bởi hai nhóm với khối lượng ca trung bình là 13,8 ca cắt gan lớn/năm. Sáu mươi chín ca cắt gan lớn đã được thực hiện cho: di căn ung thư đại trực tràng ở gan 40 (58%), di căn ngoài đại trực tràng 9 (13%), ung thư biểu mô tế bào gan 8 (11,6%), u tuyến vỡ 4 (5,8%), ung thư đường mật rốn gan 4 (5,8%), u máu 2 (2,9%), chấn thương 1 (1,5%) và u nguyên bào gan 1 (1,5%). Hai mươi mốt bệnh nhân có ít nhất một biến chứng, với tỷ lệ mắc bệnh chung là 30,4%. Có các biến chứng nhỏ ở 17 (24,6%) bệnh nhân, biến chứng lớn ở 11 (15,9%) bệnh nhân và 4 (5,8%) tử vong. KẾT LUẬN Có những hạn chế về địa lý, chính trị và tài chính riêng biệt đối với việc cung cấp dịch vụ chăm sóc sức khỏe ở Caribe. Tuy nhiên, kết quả lâm sàng có thể chấp nhận được ở các trung tâm gan mật có khối lượng thấp đã được thành lập ở Đông Caribe."} +{"idx": 1900106, "en": "BACKGROUND Transjugular intrahepatic portosystemic shunts (TIPS) can alleviate complications of portal hypertension such as ascites and variceal bleeding by decreasing the portosystemic gradient. In limited clinical situations, parallel TIPS may be only solution to alleviate either variceal bleeding or ascites secondary to portal hypertension when the primary TIPS fails to do so. Data specifically addressing the use of this partially polytetrafluoroethylene covered nitinol stent (Viatorr®) is largely lacking despite Viatorr® being the current gold standard for modern TIPS placement. CASE SUMMARY All three patients had portal hypertension and already had a primary Viatorr® TIPS placed previously. All patients have undergone failed endoscopy to manage acute variceal bleeding before referral for a parallel stent (PS). PS were placed in patients presenting with recurrent variceal bleeding despite existence of a widely patent primary TIPS. Primary stent patency was verified with either Doppler ultrasound or intra-procedural TIPS stent venography. Doppler ultrasound follow-up imaging demonstrated complete patency of both primary and parallel TIPS. All three patients did well on clinical follow-up of up to six months and no major complications were recorded. A review of existing literature on the role of PS in the management of portal hypertension complications is discussed. There are three case reports of use of primary and PS Viatorr® stents placement, only one of which is in a patient with gastrointestinal variceal bleeding despite a patent primary Viatorr® TIPS. CONCLUSION Viatorr® PS placement in the management of variceal hemorrhage is feasible with promising short term patency and clinical follow-up data.", "gemini": "BỐI CẢNH Các shunt cửa chủ trong gan xuyên tĩnh mạch (TIPS) có thể làm giảm các biến chứng của tăng áp lực tĩnh mạch cửa như cổ trướng và chảy máu giãn tĩnh mạch bằng cách giảm độ chênh áp cửa chủ. Trong các tình huống lâm sàng hạn chế, TIPS song song có thể chỉ là giải pháp để làm giảm chảy máu giãn tĩnh mạch hoặc cổ trướng thứ phát do tăng áp lực tĩnh mạch cửa khi TIPS chính không thực hiện được. Dữ liệu đề cập cụ thể đến việc sử dụng stent nitinol phủ polytetrafluoroethylene một phần này (Viatorr®) phần lớn còn thiếu mặc dù Viatorr® là tiêu chuẩn vàng hiện tại để đặt TIPS hiện đại. TÓM TẮT TRƯỜNG HỢP Cả ba bệnh nhân đều bị tăng áp lực tĩnh mạch cửa và đã được đặt TIPS Viatorr® chính trước đó. Tất cả các bệnh nhân đã nội soi thất bại để xử lý tình trạng chảy máu giãn tĩnh mạch cấp tính trước khi chuyển đến đặt stent song song (PS). PS đã được đặt ở những bệnh nhân bị chảy máu giãn tĩnh mạch tái phát mặc dù TIPS chính đã thông hoàn toàn. Độ thông của stent chính đã được xác minh bằng siêu âm Doppler hoặc chụp mạch stent TIPS trong quá trình can thiệp. Hình ảnh theo dõi siêu âm Doppler cho thấy độ thông hoàn toàn của cả TIPS chính và TIPS song song. Cả ba bệnh nhân đều có kết quả theo dõi lâm sàng tốt trong tối đa sáu tháng và không ghi nhận biến chứng lớn nào. Đánh giá các tài liệu hiện có về vai trò của PS trong việc xử lý các biến chứng tăng áp lực tĩnh mạch cửa đã được thảo luận. Có ba báo cáo trường hợp về việc sử dụng stent Viatorr® chính và PS, chỉ có một trường hợp là ở bệnh nhân bị chảy máu giãn tĩnh mạch đường tiêu hóa mặc dù TIPS Viatorr® chính đã thông. KẾT LUẬN Việc đặt PS Viatorr® trong việc xử lý xuất huyết giãn tĩnh mạch là khả thi với dữ liệu về độ thông ngắn hạn và theo dõi lâm sàng đầy hứa hẹn."} +{"idx": 1900107, "en": "BACKGROUND Necrolytic acral erythema (NAE) is a rare dermatological disorder, which is associated with hepatitis C virus (HCV) infection or zinc deficiency. It is characterized by erythematous or violaceous lesions occurring primarily in the lower extremities. The treatment includes systemic steroids and oral zinc supplementation. We report a case of NAE in a 66-year-old human immunodeficiency virus (HIV)/HCV co-infected woman with NAE. NAE is rarely reported in co-infected patients and the exact mechanisms of pathogenesis are still unclear. CASE SUMMARY A 66-year-old HIV/HCV co-infected female patient presented with painless, non-pruritic rash of extremities for one week and underwent extensive work-up for possible rheumatologic disorders including vasculitis and cryoglobulinemia. Punch skin biopsies of right and left thigh revealed thickened parakeratotic stratum corneum most consistent with NAE. Patient was started on prednisone and zinc supplementation with resolution of the lesions and improvement of rash. CONCLUSION Clinicians should maintain high clinical suspicion for early recognition of NAE in patients with rash and HCV.", "gemini": "BỐI CẢNH Hồng ban acral hoại tử (NAE) là một rối loạn da hiếm gặp, có liên quan đến nhiễm virus viêm gan C (HCV) hoặc thiếu kẽm. Nó được đặc trưng bởi các tổn thương màu hồng ban hoặc tím chủ yếu xảy ra ở các chi dưới. Việc điều trị bao gồm các loại steroid toàn thân và bổ sung kẽm đường uống. Chúng tôi báo cáo một trường hợp NAE ở một phụ nữ 66 tuổi đồng nhiễm virus suy giảm miễn dịch ở người (HIV)/HCV bị NAE. NAE hiếm khi được báo cáo ở những bệnh nhân đồng nhiễm và cơ chế sinh bệnh chính xác vẫn chưa rõ ràng. TÓM TẮT TRƯỜNG HỢP Một bệnh nhân nữ 66 tuổi đồng nhiễm HIV/HCV đã đến khám với tình trạng phát ban ở các chi không đau, không ngứa trong một tuần và đã được kiểm tra chuyên sâu để tìm các rối loạn thấp khớp có thể xảy ra bao gồm viêm mạch và bệnh cryoglobulin máu. Sinh thiết da đột lỗ ở đùi phải và trái cho thấy lớp sừng dày sừng hóa phù hợp nhất với NAE. Bệnh nhân đã bắt đầu dùng prednisone và bổ sung kẽm, các tổn thương đã khỏi và phát ban cũng cải thiện. KẾT LUẬN Các bác sĩ lâm sàng nên duy trì nghi ngờ lâm sàng cao để nhận biết sớm NAE ở những bệnh nhân bị phát ban và HCV."} +{"idx": 1900108, "en": "BACKGROUND Portal vein thrombosis (PVT) after liver transplantation (LT) is an uncommon complication with potential for significant morbidity and mortality that transplant providers should be cognizant of. Recognizing subtle changes in post-operative ultrasounds that could herald but do not definitively diagnose PVT is paramount. CASE SUMMARY A 30-year-old female with a history of alcohol-related cirrhosis presented with painless jaundice and received a deceased donor orthotopic liver transplant. On the first two days post-operatively, her liver Doppler ultrasounds showed a patent portal vein, increased hepatic arterial diastolic flows, and reduced hepatic arterial resistive indices. She was asymptomatic with improving labs. On post-operative day three, her resistive indices declined further, and computed tomography of the abdomen revealed a large extra-hepatic PVT. The patient then underwent emergent percutaneous venography with tissue plasminogen activator administration, angioplasty, and stent placement. Aspirin was started to prevent stent thrombosis. Follow-up ultrasounds showed a patent portal vein and improved hepatic arterial resistive indices. Her graft function improved to normal by discharge. Although decreased hepatic artery resistive indices and increased diastolic flows on ultrasound are often associated with hepatic arterial stenosis post-LT, PVT can also cause these findings. CONCLUSION Reduced hepatic arterial resistive indices on ultrasound can signify PVT post-LT, and thrombolysis, angioplasty, and stent placement are efficacious treatments.", "gemini": "BỐI CẢNH Huyết khối tĩnh mạch cửa (PVT) sau ghép gan (LT) là một biến chứng ít gặp có khả năng gây bệnh tật và tử vong đáng kể mà các nhà cung cấp dịch vụ ghép tạng cần lưu ý. Việc nhận biết những thay đổi nhỏ trên siêu âm sau phẫu thuật có thể báo trước nhưng không chẩn đoán chính xác PVT là điều tối quan trọng. TÓM TẮT CA BỆNH Một phụ nữ 30 tuổi có tiền sử xơ gan do rượu đã xuất hiện vàng da không đau và được ghép gan chỉnh hình từ người hiến tặng đã chết. Trong hai ngày đầu sau phẫu thuật, siêu âm Doppler gan của cô cho thấy tĩnh mạch cửa thông, tăng lưu lượng động mạch gan và giảm chỉ số cản trở động mạch gan. Cô không có triệu chứng gì với các xét nghiệm đang cải thiện. Vào ngày thứ ba sau phẫu thuật, chỉ số cản trở của cô tiếp tục giảm và chụp cắt lớp vi tính ổ bụng cho thấy một PVT ngoại gan lớn. Sau đó, bệnh nhân đã trải qua chụp tĩnh mạch qua da khẩn cấp với việc sử dụng chất hoạt hóa plasminogen mô, đặt stent và nong mạch. Aspirin được bắt đầu để ngăn ngừa huyết khối stent. Siêu âm theo dõi cho thấy tĩnh mạch cửa thông và chỉ số cản trở động mạch gan được cải thiện. Chức năng ghép của cô đã cải thiện trở lại bình thường khi xuất viện. Mặc dù chỉ số cản trở động mạch gan giảm và lưu lượng tâm trương tăng trên siêu âm thường liên quan đến hẹp động mạch gan sau ghép LT, nhưng PVT cũng có thể gây ra những phát hiện này. KẾT LUẬN Chỉ số cản trở động mạch gan giảm trên siêu âm có thể biểu thị PVT sau LT, và tiêu sợi huyết, nong mạch và đặt stent là những phương pháp điều trị hiệu quả."} +{"idx": 1900109, "en": "BACKGROUND Only one case of liver transplantation for hepatic adenoma has previously been reported for patients with rupture and uncontrolled hemorrhage. We present the case of a massive ruptured hepatic adenoma with persistent hemorrhagic shock and toxic liver syndrome which resulted in a two-stage liver transplantation. This is the first case of a two-stage liver transplantation performed for a ruptured hepatic adenoma. CASE SUMMARY A 23 years old African American female with a history of pre-diabetes and oral contraceptive presented to an outside facility complaining of right-sided chest pain and emesis for one day. She was found to be in hemorrhagic shock due to a massive ruptured hepatic hepatic adenoma. She underwent repeated embolizations with interventional radiology with ongoing hemorrhage and the development of renal failure, hepatic failure, and hemodynamic instability, known as toxic liver syndrome. In the setting of uncontrolled hemorrhage and toxic liver syndrome, a hepatectomy with porto-caval anastomosis was performed with liver transplantation 15 h later. She tolerated the anhepatic stage well, and has done well over one year later. CONCLUSION When toxic liver syndrome is recognized, liver transplantation with or without hepatectomy should be considered before the patient becomes unstable.", "gemini": "BỐI CẢNH Chỉ có một trường hợp ghép gan do u gan được báo cáo trước đây ở những bệnh nhân bị vỡ và xuất huyết không kiểm soát. Chúng tôi trình bày trường hợp u gan vỡ lớn với tình trạng sốc mất máu liên tục và hội chứng gan nhiễm độc dẫn đến ghép gan hai giai đoạn. Đây là trường hợp đầu tiên ghép gan hai giai đoạn được thực hiện cho u gan vỡ. TÓM TẮT CA BỆNH Một phụ nữ Mỹ gốc Phi 23 tuổi có tiền sử tiền tiểu đường và thuốc tránh thai uống đã đến một cơ sở bên ngoài để than phiền về đau ngực bên phải và nôn trong một ngày. Cô được phát hiện bị sốc mất máu do u gan vỡ lớn. Cô đã trải qua nhiều lần nút mạch với can thiệp mạch máu học với tình trạng xuất huyết đang diễn ra và phát triển suy thận, suy gan và mất ổn định huyết động, được gọi là hội chứng gan nhiễm độc. Trong bối cảnh xuất huyết không kiểm soát và hội chứng gan nhiễm độc, đã thực hiện cắt gan với nối cửa chủ và ghép gan sau đó 15 giờ. Cô đã dung nạp tốt giai đoạn không gan và đã ổn định sau hơn một năm. KẾT LUẬN Khi hội chứng gan nhiễm độc được phát hiện, nên cân nhắc ghép gan có hoặc không cắt gan trước khi bệnh nhân trở nên không ổn định."} +{"idx": 1900110, "en": "The two ubiquitous, outside the retina, G protein-coupled receptor (GPCR) adapter proteins, β-arrestin-1 and -2 (also known as arrestin-2 and -3, respectively), have three major functions in cells: GPCR desensitization, i.e., receptor decoupling from G-proteins; GPCR internalization via clathrin-coated pits; and signal transduction independently of or in parallel to G-proteins. Both β-arrestins are expressed in the heart and regulate a large number of cardiac GPCRs. The latter constitute the single most commonly targeted receptor class by Food and Drug Administration-approved cardiovascular drugs, with about one-third of all currently used in the clinic medications affecting GPCR function. Since β-arrestin-1 and -2 play important roles in signaling and function of several GPCRs, in particular of adrenergic receptors and angiotensin II type 1 receptors, in cardiac myocytes, they have been a major focus of cardiac biology research in recent years. Perhaps the most significant realization coming out of their studies is that these two GPCR adapter proteins, initially thought of as functionally interchangeable, actually exert diametrically opposite effects in the mammalian myocardium. Specifically, the most abundant of the two β-arrestin-1 exerts overall detrimental effects on the heart, such as negative inotropy and promotion of adverse remodeling post-myocardial infarction (MI). In contrast, β-arrestin-2 is overall beneficial for the myocardium, as it has anti-apoptotic and anti-inflammatory effects that result in attenuation of post-MI adverse remodeling, while promoting cardiac contractile function. Thus, design of novel cardiac GPCR ligands that preferentially activate β-arrestin-2 over β-arrestin-1 has the potential of generating novel cardiovascular therapeutics for heart failure and other heart diseases.", "gemini": "Hai protein thích nghi thụ thể liên kết protein G (GPCR) phổ biến bên ngoài võng mạc, β-arrestin-1 và -2 (còn được gọi lần lượt là arrestin-2 và -3), có ba chức năng chính trong tế bào: Giảm nhạy cảm GPCR, tức là giải liên kết thụ thể khỏi G-protein; Nội tại hóa GPCR thông qua hố phủ clathrin; và chuyển tín hiệu độc lập hoặc song song với G-protein. Cả β-arrestin đều được biểu hiện trong tim và điều chỉnh một số lượng lớn GPCR tim. GPCR sau này tạo nên lớp thụ thể được nhắm mục tiêu phổ biến nhất bởi các loại thuốc tim mạch được Cục Quản lý Thực phẩm và Dược phẩm chấp thuận, với khoảng một phần ba trong số tất cả các loại thuốc hiện được sử dụng trong phòng khám ảnh hưởng đến chức năng GPCR. Vì β-arrestin-1 và -2 đóng vai trò quan trọng trong việc truyền tín hiệu và chức năng của một số GPCR, đặc biệt là thụ thể adrenergic và thụ thể angiotensin II loại 1, trong các tế bào cơ tim, nên chúng đã trở thành trọng tâm chính của nghiên cứu sinh học tim trong những năm gần đây. Có lẽ nhận thức quan trọng nhất từ các nghiên cứu của họ là hai protein thích nghi GPCR này, ban đầu được coi là có thể hoán đổi chức năng, thực sự tạo ra các tác động hoàn toàn trái ngược nhau ở cơ tim của động vật có vú. Cụ thể, β-arrestin-1 dồi dào nhất trong số hai loại β-arrestin-1 có tác dụng gây hại tổng thể cho tim, chẳng hạn như giảm tính co bóp và thúc đẩy quá trình tái tạo bất lợi sau nhồi máu cơ tim (MI). Ngược lại, β-arrestin-2 có lợi tổng thể cho cơ tim, vì nó có tác dụng chống apoptosis và chống viêm dẫn đến làm giảm quá trình tái tạo bất lợi sau MI, đồng thời thúc đẩy chức năng co bóp của tim. Do đó, thiết kế các phối tử GPCR tim mới kích hoạt β-arrestin-2 tốt hơn β-arrestin-1 có khả năng tạo ra các phương pháp điều trị tim mạch mới cho suy tim và các bệnh tim khác."} +{"idx": 1900111, "en": "Patient with atrial fibrillation (AF) are at risk of developing stroke with the left atrial appendage (LAA) being the most common site for thrombus formation. If left untreated, AF is associated with 4 to 5 folds increase in the risk of ischemic stroke in all age groups. About 5% to 15% of AF patients have atrial thrombi on transesophageal echocardiography, and 91% of those thrombi are located in the LAA in patient with nonrheumatic AF. Although oral anticoagulants are the gold-standard treatment for stroke prevention in patients with non-valvular AF, some patients are at high risk of bleeding and deemed not candidates for anticoagulation. Therefore, LAA occlusion (LAAO) has emerged as alternative approach for stroke prevention in those patients. Surgical LAAO is associated with high rate of unsuccessful closure and recommended only in patients with AF and undergoing cardiac surgery. Percutaneous LAAO uses transvenous access with trans-septal puncture and was first tested using the PLAATO device. Watchman is the most common and only Food and Drug Administration (FDA) approved device for LAAO. LAAO using Watchman device is non-inferior to warfarin therapy in preventing ischemic stroke/systemic thromboembolism. However, it is associated with lower rates of hemorrhagic stroke, bleeding and death. Amplatzer is another successful LAAO device that has CE mark and is waiting for FDA approval. Optimal antithrombotic therapy post LAAO is still under debate and highly patient-specific. The aim of this paper is to systematically review the current literature to evaluate the efficacy and safety of different LAAO devices.", "gemini": "Bệnh nhân bị rung nhĩ (AF) có nguy cơ bị đột quỵ với phần phụ nhĩ trái (LAA) là vị trí phổ biến nhất để hình thành huyết khối. Nếu không được điều trị, AF có liên quan đến nguy cơ đột quỵ do thiếu máu cục bộ tăng từ 4 đến 5 lần ở mọi nhóm tuổi. Khoảng 5% đến 15% bệnh nhân AF có huyết khối nhĩ trên siêu âm tim qua thực quản và 91% trong số các huyết khối đó nằm ở LAA ở bệnh nhân AF không do thấp khớp. Mặc dù thuốc chống đông đường uống là phương pháp điều trị tiêu chuẩn vàng để phòng ngừa đột quỵ ở những bệnh nhân bị AF không do van tim, một số bệnh nhân có nguy cơ chảy máu cao và được coi là không đủ điều kiện để chống đông. Do đó, tắc LAA (LAAO) đã nổi lên như một phương pháp tiếp cận thay thế để phòng ngừa đột quỵ ở những bệnh nhân đó. LAAO phẫu thuật có tỷ lệ đóng không thành công cao và chỉ được khuyến cáo ở những bệnh nhân bị AF và phải phẫu thuật tim. LAAO qua da sử dụng đường tiếp cận qua tĩnh mạch với thủ thuật chọc xuyên vách và được thử nghiệm lần đầu tiên bằng thiết bị PLAATO. Watchman là thiết bị phổ biến nhất và là thiết bị duy nhất được Cục Quản lý Thực phẩm và Dược phẩm (FDA) chấp thuận cho LAAO. LAAO sử dụng thiết bị Watchman không kém hơn so với liệu pháp warfarin trong việc ngăn ngừa đột quỵ do thiếu máu cục bộ/thuyên tắc huyết khối toàn thân. Tuy nhiên, nó có liên quan đến tỷ lệ đột quỵ do xuất huyết, chảy máu và tử vong thấp hơn. Amplatzer là một thiết bị LAAO thành công khác có dấu CE và đang chờ FDA chấp thuận. Liệu pháp chống huyết khối tối ưu sau LAAO vẫn đang được tranh luận và phụ thuộc nhiều vào từng bệnh nhân. Mục đích của bài báo này là xem xét một cách có hệ thống các tài liệu hiện tại để đánh giá hiệu quả và độ an toàn của các thiết bị LAAO khác nhau."} +{"idx": 1900112, "en": "The incidence of heart valve disease increases significantly with age. Degenerative abnormalities associated with severe aortic stenosis and mitral and tricuspid regurgitation are found in not less than 10% of the population aged ≥ 75 years. Surgical treatment has been considered for years to be the treatment of choice. However, it was not uncommonly associated with high perioperative morbidity and mortality due to frequent comorbidities and overall frailty conditions of these patients. Conventional risk scores such as Society of Thoracic Surgeons and European System for Cardiac Operative Risk Evaluation may underestimate the risk of surgery in elderly patients, leading to inappropriate surgical indication. On the other hand, at least 30% of patients with severe conditions are left untreated due to prohibitive surgical risk. Interventional procedures, which are in continuous development, may be actually considered for high risk patients and, as recent results suggest, also for intermediate risk patients.", "gemini": "Tỷ lệ mắc bệnh van tim tăng đáng kể theo tuổi tác. Những bất thường thoái hóa liên quan đến hẹp động mạch chủ nặng và hở van hai lá và van ba lá được tìm thấy ở không dưới 10% dân số từ 75 tuổi trở lên. Điều trị phẫu thuật đã được coi là phương pháp điều trị được lựa chọn trong nhiều năm. Tuy nhiên, nó thường liên quan đến tỷ lệ mắc bệnh và tử vong trong thời kỳ hậu phẫu cao do các bệnh đi kèm thường gặp và tình trạng suy nhược chung của những bệnh nhân này. Các điểm số rủi ro thông thường như Hiệp hội Phẫu thuật Lồng ngực và Hệ thống Đánh giá Rủi ro Phẫu thuật Tim mạch Châu Âu có thể đánh giá thấp rủi ro phẫu thuật ở bệnh nhân cao tuổi, dẫn đến chỉ định phẫu thuật không phù hợp. Mặt khác, ít nhất 30% bệnh nhân bị tình trạng nặng không được điều trị do nguy cơ phẫu thuật quá cao. Các thủ thuật can thiệp đang liên tục được phát triển, thực tế có thể được cân nhắc cho những bệnh nhân có nguy cơ cao và như kết quả gần đây cho thấy, cũng có thể dành cho những bệnh nhân có nguy cơ trung bình."} +{"idx": 1900113, "en": "Purpose To assess the effect of a novel intense pulsed light (IPL) therapy on tear proteins and lipids in eyes with Meibomian gland dysfunction (MGD). Methods Twenty-four eyes of 12 patients with MGD were recruited and received five overlapping flashes (565-1400 nm) directed at the lower eyelid. The IPL parameters include intensity: 2.5 to 6.5 J/cm2, voltage: 100 to 240 V, frequency: 50 to 60 Hz, input: 16 W, maximum optical energy: 23 J, pulse duration: <2.0 ms, and repetition time: 1-3.5 s. Tear samples were evaluated immediately before and 2 weeks after IPL therapy and included measurements of protein concentration, electrophoretic mobility by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, lipid profile assessments, and thin-layer chromatography (TLC) for phospholipids. Results Significant improvements were observed in tear protein concentrations and molecular weight after IPL therapy. The most pronounced effect was in the molecular weight of tear lysozyme, lactoferrin, and albumin. Tear lipids showed an improvement in the concentrations of total lipids, triglycerides, cholesterol, and phospholipids. On TLC, the tears in patients with MGD had significantly lower amounts of anionic phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine but amounts zwitterionic neutral phospholipid phosphatidylcholine were normal. These anionic phospholipids showed obvious recovery after IPL therapy. Conclusion IPL therapy is effective in eyes with MGD. It improved tear protein and lipid content and composition. The anionic phospholipids were more responsive to IPL therapy than were the other zwitterionic phospholipids.", "gemini": "Mục đích Đánh giá hiệu quả của liệu pháp ánh sáng xung cường độ cao (IPL) mới đối với protein và lipid nước mắt ở mắt bị rối loạn chức năng tuyến Meibomian (MGD). Phương pháp Hai mươi bốn mắt của 12 bệnh nhân bị MGD đã được tuyển dụng và nhận được năm xung chồng lên nhau (565-1400 nm) hướng vào mí mắt dưới. Các thông số IPL bao gồm cường độ: 2,5 đến 6,5 J/cm2, điện áp: 100 đến 240 V, tần số: 50 đến 60 Hz, đầu vào: 16 W, năng lượng quang tối đa: 23 J, thời lượng xung: <2,0 ms và thời gian lặp lại: 1-3,5 giây. Các mẫu nước mắt được đánh giá ngay trước và 2 tuần sau khi điều trị IPL và bao gồm các phép đo nồng độ protein, độ di động điện di bằng phương pháp điện di gel polyacrylamide natri dodecyl sulfat, đánh giá hồ sơ lipid và sắc ký lớp mỏng (TLC) đối với phospholipid. Kết quả Cải thiện đáng kể đã được quan sát thấy ở nồng độ protein nước mắt và trọng lượng phân tử sau khi điều trị IPL. Hiệu quả rõ rệt nhất là ở trọng lượng phân tử của lysozyme nước mắt, lactoferrin và albumin. Lipid nước mắt cho thấy sự cải thiện về nồng độ tổng lipid, triglycerid, cholesterol và phospholipid. Trên TLC, nước mắt ở những bệnh nhân bị MGD có lượng phosphatidylethanolamine anion, phosphatidylinositol và phosphatidylserine thấp hơn đáng kể nhưng lượng phosphatidylcholine phospholipid trung tính zwitterion là bình thường. Những phospholipid anion này cho thấy sự phục hồi rõ ràng sau khi điều trị IPL. Kết luận Liệu pháp IPL có hiệu quả đối với mắt bị MGD. Nó cải thiện hàm lượng và thành phần protein và lipid nước mắt. Các phospholipid anion phản ứng nhiều hơn với liệu pháp IPL so với các phospholipid zwitterion khác."} +{"idx": 1900114, "en": "Purpose To evaluate sensitivity and specificity of digital retinal image reading in the diagnosis of referral-warranted retinopathy of prematurity (ROP). Methods Infants referred to the ROP clinic underwent fundus examination through indirect ophthalmoscopy. Fundus photographs were acquired using RetCam (shuttle 2; Clarity medical systems, Pleasanton, CA, USA). Four retinal specialists who were blind to patients' information reviewed the RetCam fundus photographs. By comparing the results of photographs' readings with that of indirect ophthalmoscopy as the gold standard, the sensitivity and specificity of telescreening was determined. Results A total of 147 treatment-naïve patients met the inclusion criteria and were enrolled in the study. Mean gestational age (GA) was 28.6 ± 2.0 weeks. Digital retinal imaging had sensitivity of 85% and specificity of 35% in detecting referral-warranted ROP in our study. Positive predictive value of digital photography was 80%, and negative predictive value was 43%. Conclusion Digital photography for diagnosis of ROP may show good potential as a screening modality in developing countries. It can facilitate early diagnosis, prevent unnecessary referrals, and be implemented for investigational purpose. However, the overall study result did not provide evidence to propose digital photography as a substitute for indirect ophthalmoscopy in the diagnosis of ROP.", "gemini": "Mục đích Đánh giá độ nhạy và độ đặc hiệu của việc đọc ảnh võng mạc kỹ thuật số trong chẩn đoán bệnh võng mạc của trẻ sinh non (ROP) cần được chuyển tuyến. Phương pháp Trẻ sơ sinh được chuyển đến phòng khám ROP đã được khám đáy mắt thông qua soi đáy mắt gián tiếp. Ảnh chụp đáy mắt được chụp bằng RetCam (shuttle 2; hệ thống y tế Clarity, Pleasanton, CA, Hoa Kỳ). Bốn chuyên gia võng mạc không biết thông tin của bệnh nhân đã xem xét các bức ảnh chụp đáy mắt RetCam. Bằng cách so sánh kết quả đọc ảnh với kết quả soi đáy mắt gián tiếp như tiêu chuẩn vàng, độ nhạy và độ đặc hiệu của việc tầm soát từ xa đã được xác định. Kết quả Tổng cộng có 147 bệnh nhân chưa được điều trị đáp ứng các tiêu chuẩn tuyển chọn và được đưa vào nghiên cứu. Tuổi thai trung bình (GA) là 28,6 ± 2,0 tuần. Chụp võng mạc kỹ thuật số có độ nhạy là 85% và độ đặc hiệu là 35% trong việc phát hiện ROP cần được chuyển tuyến trong nghiên cứu của chúng tôi. Giá trị dự đoán dương tính của chụp ảnh kỹ thuật số là 80% và giá trị dự đoán âm tính là 43%. Kết luận Chụp ảnh kỹ thuật số để chẩn đoán ROP có thể cho thấy tiềm năng tốt như một phương thức sàng lọc ở các nước đang phát triển. Nó có thể giúp chẩn đoán sớm, ngăn ngừa các lần chuyển tuyến không cần thiết và được triển khai cho mục đích điều tra. Tuy nhiên, kết quả nghiên cứu tổng thể không đưa ra bằng chứng để đề xuất chụp ảnh kỹ thuật số thay thế cho soi đáy mắt gián tiếp trong chẩn đoán ROP."} +{"idx": 1900115, "en": "Purpose To review the management outcomes of black diaphragm intraocular (BDI) lens implantation in Arab patients with aniridia. Methods Patients with aniridia undergone BDI lens implantation at our institution between 2013 and 2014 were included. Uncorrected visual acuity (UCVA), manifest refraction, and best-corrected visual acuity (BCVA) were evaluated before and 6 months and yearly after BDI lens implant surgery until the last visit. Intra- and postoperative complications were noted. Results Our series comprised 14 patients (8 males) with aniridia. The median duration of follow-up was 30 months (25% quartile). Ocular parameters, refractive status, and vison were all significantly improved at the last follow-up compared to the preoperative values (P < 0.05 for all comparisons). All patients reported a significant decrease in photophobia and glare. Postoperatively, 11 eyes (78%) gained 2 or more lines of UCVA. At the last follow-up, BCVA increased by 2 or more lines in all cases. Early postoperative complications included main wound leakage (one eye) and anterior chamber hyphema (one eye). Late (≥6 months) complications included corneal decompensation (one eye), failed penetrating keratoplasty graft (2 eyes), and subluxation of a scleral fixated BDI lens (one eye). Surgical interventions performed to manage complications included penetrating keratoplasty in 2 eyes with corneal decompensation and failed graft (one each), and re-suturing of a subluxated intraocular lens (one eye). Conclusion BDI lenses seem to be a safe and effective iris prosthetic with intraocular lens combination surgery for patients with congenital or traumatic aniridia. Periodic evaluation and prompt management of complications are recommended.", "gemini": "Mục đích Xem xét kết quả điều trị của phẫu thuật cấy ghép thủy tinh thể nội nhãn màng chắn đen (BDI) ở những bệnh nhân người Ả Rập bị chứng không có mống mắt. Phương pháp Những bệnh nhân bị không có mống mắt đã được cấy ghép thủy tinh thể BDI tại cơ sở của chúng tôi trong khoảng thời gian từ năm 2013 đến năm 2014 đã được đưa vào nghiên cứu. Đo thị lực không chỉnh kính (UCVA), khúc xạ biểu hiện và thị lực được chỉnh kính tốt nhất (BCVA) đã được đánh giá trước và 6 tháng và hàng năm sau phẫu thuật cấy ghép thủy tinh thể BDI cho đến lần khám cuối. Các biến chứng trong và sau phẫu thuật đã được ghi nhận. Kết quả Loạt nghiên cứu của chúng tôi bao gồm 14 bệnh nhân (8 nam) bị không có mống mắt. Thời gian theo dõi trung bình là 30 tháng (tứ phân vị 25%). Các thông số về mắt, tình trạng khúc xạ và thị lực đều được cải thiện đáng kể ở lần theo dõi cuối cùng so với các giá trị trước phẫu thuật (P < 0,05 cho tất cả các so sánh). Tất cả bệnh nhân đều báo cáo rằng chứng sợ ánh sáng và chói sáng giảm đáng kể. Sau phẫu thuật, 11 mắt (78%) đã tăng 2 hoặc nhiều dòng UCVA. Ở lần theo dõi cuối cùng, BCVA tăng 2 hoặc nhiều dòng trong tất cả các trường hợp. Các biến chứng sau phẫu thuật sớm bao gồm rò rỉ vết thương chính (một mắt) và tụ máu tiền phòng (một mắt). Các biến chứng muộn (≥6 tháng) bao gồm mất bù giác mạc (một mắt), ghép giác mạc xuyên thấu không thành công (2 mắt) và bán trật vị trí của thủy tinh thể BDI cố định ở củng mạc (một mắt). Các can thiệp phẫu thuật được thực hiện để xử lý các biến chứng bao gồm ghép giác mạc xuyên thấu ở 2 mắt bị mất bù giác mạc và ghép không thành công (mỗi mắt một) và khâu lại thủy tinh thể nội nhãn bị bán trật vị trí (một mắt). Kết luận Thủy tinh thể BDI dường như là một vật liệu thay thế mống mắt an toàn và hiệu quả với phẫu thuật kết hợp thủy tinh thể nội nhãn cho những bệnh nhân bị không có mống mắt bẩm sinh hoặc chấn thương. Nên đánh giá định kỳ và xử lý kịp thời các biến chứng."} +{"idx": 1900116, "en": "Purpose To evaluate the effects of donor graft thickness on postoperative best spectacle-corrected visual acuity (BSCVA), refractive outcomes, endothelial cell density (ECD) and function, intraocular pressure (IOP), and postoperative complications after Descemet stripping automated endothelial keratoplasty (DSAEK). Methods This retrospective, interventional case series enrolled 77 eyes of 64 patients who underwent DSAEK with or without simultaneous cataract surgery. Clinical outcomes, including BSCVA, refraction, keratometric astigmatism, IOP, and ECD were assessed at the final follow-up examination. Univariate analyses were used to investigate the effects of postoperative donor graft thickness on clinical outcomes and complications. Results The mean patient age was 62.3 ± 15.6 years, and the patients were followed for 26.2 ± 20.9 months postoperatively. The mean postoperative central graft thickness was 102.4 ± 31.6 μm. In the univariate analysis, postoperative central graft thickness was significantly associated with postoperative IOP (P = 0.005), central recipient thickness (P = 0.002), and ECD (P = 0.016). No significant association was found for central graft thickness with postoperative BSCVA (P = 0.70), spherical equivalent refraction (P = 0.33), keratometric astigmatism (P = 0.27), graft detachment (P = 0.16), graft decentration (P = 0.17), high IOP (P = 0.53), or endothelial rejection (P = 0.88). Conclusion This study failed to demonstrate any significant correlation between graft thickness and BSCVA. Attempting to minimize graft thickness might not have the desired outcome regarding endothelial cell density and function. Increased graft thickness could negatively impact the accuracy of IOP measurements after DSAEK.", "gemini": "Mục đích Đánh giá ảnh hưởng của độ dày ghép của người hiến đối với thị lực được chỉnh kính tốt nhất sau phẫu thuật (BSCVA), kết quả khúc xạ, mật độ tế bào nội mạc (ECD) và chức năng, nhãn áp (IOP) và các biến chứng sau phẫu thuật sau phẫu thuật cắt bỏ lớp Descemet tự động ghép nội mạc giác mạc (DSAEK). Phương pháp Loạt ca can thiệp hồi cứu này đã tuyển dụng 77 mắt của 64 bệnh nhân đã trải qua DSAEK có hoặc không có phẫu thuật đục thủy tinh thể đồng thời. Các kết quả lâm sàng, bao gồm BSCVA, khúc xạ, loạn thị giác giác mạc, IOP và ECD đã được đánh giá tại lần khám theo dõi cuối cùng. Các phân tích đơn biến đã được sử dụng để điều tra ảnh hưởng của độ dày ghép của người hiến sau phẫu thuật đối với các kết quả lâm sàng và biến chứng. Kết quả Độ tuổi trung bình của bệnh nhân là 62,3 ± 15,6 tuổi và bệnh nhân được theo dõi trong 26,2 ± 20,9 tháng sau phẫu thuật. Độ dày ghép trung tâm trung bình sau phẫu thuật là 102,4 ± 31,6 μm. Trong phân tích đơn biến, độ dày ghép trung tâm sau phẫu thuật có liên quan đáng kể với IOP sau phẫu thuật (P = 0,005), độ dày trung tâm của người nhận (P = 0,002) và ECD (P = 0,016). Không tìm thấy mối liên hệ đáng kể nào giữa độ dày ghép trung tâm với BSCVA sau phẫu thuật (P = 0,70), khúc xạ tương đương cầu (P = 0,33), loạn thị giác giác mạc (P = 0,27), ghép tách rời (P = 0,16), ghép lệch tâm (P = 0,17), IOP cao (P = 0,53) hoặc thải ghép nội mạc (P = 0,88). Kết luận Nghiên cứu này không chứng minh được bất kỳ mối tương quan đáng kể nào giữa độ dày ghép và BSCVA. Cố gắng giảm thiểu độ dày ghép có thể không đạt được kết quả mong muốn về mật độ và chức năng tế bào nội mạc. Độ dày ghép tăng có thể ảnh hưởng tiêu cực đến độ chính xác của các phép đo IOP sau DSAEK."} +{"idx": 1900117, "en": "Purpose To evaluate the peripapillary choroidal thickness (CT) in children with unilateral amblyopia using spectral-domain optical coherence tomography (SD-OCT). Methods One hundred and six eyes of 53 children with unilateral amblyopia and 20 eyes of 20 children with normal vision were involved in this study. Of the 53 children with unilateral amblyopia, 29 (54.7%) had hyperopic anisometropic amblyopia and 24 (45.3%) had strabismic amblyopia. Peripapillary CT was measured from 6 mm length radial B-scans at the optic nerve head using the enhanced depth imaging program of an SD-OCT (Heidelberg Engineering, Germany). Age, sex, refractive error, and best-corrected visual acuity were also recorded. Results The average peripapillary CT was greater in amblyopic eyes than in the fellow eyes of the children with amblyopia (P = 0.002), and control eyes (P < 0.001). There was no significant difference between the fellow eyes of children with amblyopia and the control eyes (P = 0.158). The average peripapillary CT was negatively correlated with axial length (AL) in amblyopic eyes (r = -0.381; P = 0.005) and fellow eyes (r = -0.392; P = 0.004) but not in control eyes (r = -0.232; P = 0.325). After adjustment for the possible effects of AL, the average peripapillary CT in amblyopic eyes was still greater than in fellow eyes (P = 0.014) and control eyes (P = 0.022). Conclusion The peripapillary choroid of eyes with amblyopia was thicker than that of the fellow eyes and control eyes. No significant difference was observed between fellow eyes and control eyes.", "gemini": "Mục đích Đánh giá độ dày màng mạch quanh gai thị (CT) ở trẻ em bị lé lác đơn phương bằng phương pháp chụp cắt lớp quang học miền phổ (SD-OCT). Phương pháp Một trăm sáu mắt của 53 trẻ em bị lé lác đơn phương và 20 mắt của 20 trẻ em có thị lực bình thường đã tham gia nghiên cứu này. Trong số 53 trẻ em bị lé lác đơn phương, 29 (54,7%) bị lé lác anisometropic tăng thị và 24 (45,3%) bị lé lác lác. CT quanh gai thị được đo từ 6 mm chiều dài quét B xuyên tâm tại đầu dây thần kinh thị giác bằng chương trình hình ảnh độ sâu nâng cao của SD-OCT (Heidelberg Engineering, Đức). Tuổi, giới tính, tật khúc xạ và thị lực tốt nhất sau khi chỉnh kính cũng được ghi lại. Kết quả CT quanh gai thị trung bình ở mắt lé lác lớn hơn ở mắt đồng bên của trẻ bị lé lác (P = 0,002) và mắt đối chứng (P <0,001). Không có sự khác biệt đáng kể giữa mắt đồng bên của trẻ bị lé lác và mắt đối chứng (P = 0,158). CT quanh gai thị trung bình có tương quan nghịch với chiều dài trục (AL) ở mắt lé lác (r = -0,381; P = 0,005) và mắt đồng bên (r = -0,392; P = 0,004) nhưng không có ở mắt đối chứng (r = -0,232 ; P = 0,325). Sau khi điều chỉnh các tác động có thể có của AL, CT quanh gai thị trung bình ở mắt lé lác vẫn lớn hơn ở mắt đồng bên (P = 0,014) và mắt đối chứng (P = 0,022). Kết luận Màng mạch quanh gai thị của mắt bị lé lác dày hơn so với mắt đồng bên và mắt đối chứng. Không có sự khác biệt đáng kể nào được quan sát thấy giữa mắt đồng bên và mắt đối chứng."} +{"idx": 1900118, "en": "Purpose To investigate a potential link between the incidence of malignant glaucoma after cataract surgery and seasonal variations in daylight. Methods In total, 18,374 uncomplicated cataract surgeries were performed between June 2008 and June 2013 at an ambulatory surgery center in Toronto. Toronto's average monthly daylight over that time period-in hours per day for each month-was determined. The number of malignant glaucoma cases that developed after cataract surgery performed in months with above average daylight was compared to the number of cases that developed after cataract surgery performed in months with below average daylight. Fisher's exact test was used to analyze the relationship between the development of malignant glaucoma and variation in daylight during the month of cataract surgery. Results Malignant glaucoma developed in 16 eyes. Thirteen cases of malignant glaucoma developed in months with above average daylight and three cases developed in months with below average daylight (P = 0.01). Eyes that developed malignant glaucoma in months with more daylight were slightly longer (21.95 ± 1.23 mm) than those that developed malignant glaucoma in months with less daylight (21.55 ± 0.88 mm). Conclusion Light-induced choroidal expansion may play a major role in the development of malignant glaucoma following cataract surgery.", "gemini": "Mục đích Điều tra mối liên hệ tiềm ẩn giữa tỷ lệ mắc bệnh tăng nhãn áp ác tính sau phẫu thuật đục thủy tinh thể và sự thay đổi theo mùa của ánh sáng ban ngày. Phương pháp Tổng cộng, 18.374 ca phẫu thuật đục thủy tinh thể không biến chứng đã được thực hiện trong khoảng thời gian từ tháng 6 năm 2008 đến tháng 6 năm 2013 tại một trung tâm phẫu thuật ngoại trú ở Toronto. Ánh sáng ban ngày trung bình hàng tháng của Toronto trong khoảng thời gian đó - tính bằng giờ mỗi ngày cho mỗi tháng - đã được xác định. Số ca tăng nhãn áp ác tính phát triển sau phẫu thuật đục thủy tinh thể được thực hiện trong những tháng có ánh sáng ban ngày cao hơn mức trung bình được so sánh với số ca phát triển sau phẫu thuật đục thủy tinh thể được thực hiện trong những tháng có ánh sáng ban ngày thấp hơn mức trung bình. Kiểm định chính xác của Fisher được sử dụng để phân tích mối quan hệ giữa sự phát triển của bệnh tăng nhãn áp ác tính và sự thay đổi của ánh sáng ban ngày trong tháng phẫu thuật đục thủy tinh thể. Kết quả Bệnh tăng nhãn áp ác tính phát triển ở 16 mắt. Mười ba trường hợp tăng nhãn áp ác tính phát triển trong những tháng có ánh sáng ban ngày cao hơn mức trung bình và ba trường hợp phát triển trong những tháng có ánh sáng ban ngày thấp hơn mức trung bình (P = 0,01). Những mắt phát triển bệnh tăng nhãn áp ác tính trong những tháng có nhiều ánh sáng ban ngày dài hơn một chút (21,95 ± 1,23 mm) so với những mắt phát triển bệnh tăng nhãn áp ác tính trong những tháng có ít ánh sáng ban ngày hơn (21,55 ± 0,88 mm). Kết luận Sự giãn nở màng mạch do ánh sáng có thể đóng vai trò chính trong sự phát triển của bệnh tăng nhãn áp ác tính sau phẫu thuật đục thủy tinh thể."} +{"idx": 1900119, "en": "Marfan syndrome is an autosomal dominant genetic connective tissue disorder that results from mutations in the fibrillin-1 gene located on chromosome band 15q15-21. Fibrillin, a glycoprotein, is widely expressed throughout the body and contributes to the elasticity and force-bearing capacity of connective tissue. In the eye, fibrillin is a key constituent of the ciliary zonules, which suspend the crystalline lens in place. The zonular defect leads to ectopia lentis, which is a hallmark of Marfan ocular abnormalities and occurs in 60% to 80% of cases. Other less common ocular features of Marfan syndrome are increased axial length, astigmatism, and flat cornea. Visual function in Marfan syndrome could be affected in several ways: ectopia lentis, refractive error, amblyopia, retinal detachment, cataract, and glaucoma. Management of a subluxated lens starts with the correction of refractive error with eyeglasses in mild cases. In more severe cases, especially when the lens bisects the pupil, complete correction of refractive error is impossible without removing the subluxated lens. The best method for visual rehabilitation after lens extraction is still debated. Aphakic Artisan lens implantation at the time of subluxated lens removal results in good visual outcomes with an acceptable safety profile. Studies with longer term follow-up and larger sample populations are needed to evaluate the safety of this procedure in patients with Marfan syndrome.", "gemini": "Hội chứng Marfan là một rối loạn mô liên kết di truyền trội trên nhiễm sắc thể thường do đột biến ở gen fibrillin-1 nằm trên dải nhiễm sắc thể 15q15-21. Fibrillin, một glycoprotein, được biểu hiện rộng rãi khắp cơ thể và góp phần vào độ đàn hồi và khả năng chịu lực của mô liên kết. Trong mắt, fibrillin là thành phần chính của dây chằng treo thể mi, giữ cố định thủy tinh thể. Khiếm khuyết của dây chằng treo dẫn đến chứng lệch thủy tinh thể, đây là đặc điểm của các bất thường về mắt của Marfan và xảy ra ở 60% đến 80% các trường hợp. Các đặc điểm về mắt ít phổ biến hơn khác của hội chứng Marfan là chiều dài trục tăng, loạn thị và giác mạc phẳng. Chức năng thị giác trong hội chứng Marfan có thể bị ảnh hưởng theo một số cách: lệch thủy tinh thể, tật khúc xạ, lé lác, bong võng mạc, đục thủy tinh thể và tăng nhãn áp. Quản lý thủy tinh thể bán trật bắt đầu bằng việc điều chỉnh tật khúc xạ bằng kính đeo mắt trong các trường hợp nhẹ. Trong những trường hợp nghiêm trọng hơn, đặc biệt là khi thủy tinh thể chia đôi đồng tử, không thể điều chỉnh hoàn toàn tật khúc xạ nếu không loại bỏ thủy tinh thể bán trật. Phương pháp phục hồi thị lực tốt nhất sau khi lấy thủy tinh thể vẫn còn gây tranh cãi. Cấy ghép thủy tinh thể Artisan Aphakic tại thời điểm cắt bỏ thủy tinh thể bán trật mang lại kết quả thị lực tốt với hồ sơ an toàn có thể chấp nhận được. Các nghiên cứu có thời gian theo dõi dài hơn và quần thể mẫu lớn hơn là cần thiết để đánh giá tính an toàn của thủ thuật này ở những bệnh nhân mắc hội chứng Marfan."} +{"idx": 1900120, "en": "Purpose To assess the effect of experimental anisometropia and monovision on stereopsis using the Titmus, Randot, and TNO stereoacuity tests. Methods Sixty adult volunteers were enrolled in the present study. Four different types of anisometropia-myopia, hyperopia, and astigmatism (both 90° and 45°)-were induced by placing trial lenses over the dominant eye (from 3 to 1 D). Stereoacuity was measured using the Titmus, Randot, and TNO tests. Results In all the anisometropia types, stereopsis deteriorated with increase in anisometropia in the three stereoacuity tests performed (P < 0.001). The largest decrease in stereopsis was attributed to 3 D myopic anisometropia-6.51 ± 2.10, 6.59 ± 2.35, and 7.36 ± 1.89 arc seconds in Titmus circles, Randot circles, and TNO, respectively. Minimal change in stereopsis was observed in 1 D astigmatism of 45°. Conclusion Any type of anisometropia may reduce stereoacuity; this reduction is most noticeable with myopic anisometropia, especially in the TNO test, probably due to the lack of monocular cues.", "gemini": "Mục đích Đánh giá tác động của chứng loạn thị thực nghiệm và đơn thị trên thị lực lập thể bằng cách sử dụng các bài kiểm tra thị lực lập thể Titmus, Randot và TNO. Phương pháp Sáu mươi người tình nguyện trưởng thành đã được ghi danh vào nghiên cứu hiện tại. Bốn loại loạn thị khác nhau - cận thị, viễn thị và loạn thị (cả 90° và 45°) - được gây ra bằng cách đặt thấu kính thử trên mắt thuận (từ 3 đến 1 D). Thị lực lập thể được đo bằng các bài kiểm tra Titmus, Randot và TNO. Kết quả Trong tất cả các loại loạn thị, thị lực lập thể đều xấu đi khi loạn thị tăng trong ba bài kiểm tra thị lực lập thể được thực hiện (P < 0,001). Giảm thị lực lập thể lớn nhất là do loạn thị cận thị 3 D - 6,51 ± 2,10, 6,59 ± 2,35 và 7,36 ± 1,89 giây cung trong các vòng tròn Titmus, vòng tròn Randot và TNO, tương ứng. Sự thay đổi tối thiểu về thị lực lập thể được quan sát thấy ở loạn thị 1 D ở 45°. Kết luận Bất kỳ loại loạn thị nào cũng có thể làm giảm thị lực lập thể; sự giảm này đáng chú ý nhất đối với loạn thị cận thị, đặc biệt là trong bài kiểm tra TNO, có thể là do thiếu các tín hiệu đơn nhãn."} +{"idx": 1900121, "en": "Age-related macular degeneration (AMD) is a major cause of vision loss in the developed world and its pathogenesis is a topic of active research. To date, much study has been focused on the role of the retinal pigment epithelium (RPE) and Bruch's membrane (BrM) in AMD pathogenesis, but the role of the choroid has also been investigated. In this review, we focus on recent advancements in research in the role of the choroid in AMD, beginning with an exploration of the histopathologic, cellular and molecular changes that occur in the choroid in AMD and concluding by discussing new choroidal imaging techniques and patterns seen on fluorescein angiography, indocyanine green angiography, spectral-domain optical coherence tomography and optical coherence tomography angiography. Exploring these domains will lead to a better understanding of the factors at play beyond the outer retina in this important disease.", "gemini": "Thoái hóa điểm vàng do tuổi tác (AMD) là nguyên nhân chính gây mất thị lực ở các nước phát triển và bệnh sinh của nó là chủ đề nghiên cứu tích cực. Cho đến nay, nhiều nghiên cứu đã tập trung vào vai trò của biểu mô sắc tố võng mạc (RPE) và màng Bruch (BrM) trong bệnh sinh của AMD, nhưng vai trò của màng mạch cũng đã được nghiên cứu. Trong bài đánh giá này, chúng tôi tập trung vào những tiến bộ gần đây trong nghiên cứu về vai trò của màng mạch trong AMD, bắt đầu bằng việc khám phá những thay đổi về mô bệnh học, tế bào và phân tử xảy ra ở màng mạch trong AMD và kết thúc bằng việc thảo luận về các kỹ thuật chụp màng mạch mới và các mô hình được thấy trên chụp mạch huỳnh quang, chụp mạch xanh indocyanine, chụp cắt lớp quang học miền phổ và chụp mạch cắt lớp quang học. Khám phá những lĩnh vực này sẽ dẫn đến sự hiểu biết tốt hơn về các yếu tố có ảnh hưởng ngoài võng mạc ngoài trong căn bệnh quan trọng này."} +{"idx": 1900122, "en": "Two questions that clinicians should answer as they attempt to apply the results of clinical trials to clinical practice are: (1) will statistically significant results be reproduced in their clinical practice; and (2) if nothing goes wrong in a clinical trial, is everything alright? Regarding the first question, when considering the results of a randomized, multicenter, prospective, controlled clinical trial, two questions that cannot be addressed by simply by reading the trial results and that only the practicing clinician can answer are: (1) is the study population representative of the patient about to be treated; and (2) is the totality of evidence outside the trial (including the clinician's own extensive experience) consistent with the trial result? Regarding the second question, clinicians are advised to recognize that most studies, even Phase 3 trials, are underpowered to accurately assess the risk of low frequency events.", "gemini": "Hai câu hỏi mà các bác sĩ lâm sàng nên trả lời khi cố gắng áp dụng kết quả của các thử nghiệm lâm sàng vào thực hành lâm sàng là: (1) liệu kết quả có ý nghĩa thống kê có được tái hiện trong thực hành lâm sàng của họ hay không; và (2) nếu không có gì sai sót trong một thử nghiệm lâm sàng, thì mọi thứ có ổn không? Về câu hỏi đầu tiên, khi xem xét kết quả của một thử nghiệm lâm sàng có đối chứng, ngẫu nhiên, đa trung tâm, có triển vọng, có hai câu hỏi không thể giải quyết chỉ bằng cách đọc kết quả thử nghiệm và chỉ bác sĩ lâm sàng thực hành mới có thể trả lời được: (1) quần thể nghiên cứu có phải là đại diện của bệnh nhân sắp được điều trị không; và (2) toàn bộ bằng chứng bên ngoài thử nghiệm (bao gồm cả kinh nghiệm sâu rộng của chính bác sĩ lâm sàng) có phù hợp với kết quả thử nghiệm không? Về câu hỏi thứ hai, các bác sĩ lâm sàng được khuyên nên nhận ra rằng hầu hết các nghiên cứu, ngay cả các thử nghiệm Giai đoạn 3, đều không đủ năng lực để đánh giá chính xác rủi ro của các sự kiện tần suất thấp."} +{"idx": 1900123, "en": "Purpose To design a screening program for prevention of blindness at the community level in Iran. Methods In this qualitative study, the components and properties of the screening program were identified using a participatory action research method with focus group meetings (FGMs) with relevant health care providers and authorities. A content analysis approach was used for data analysis. Results In total, 18 stakeholders including six ophthalmologists with different sub-specialties participated in the five FGMs. The screening program aims to discover vision-threatening eye conditions in people aged 50 years and over. Primary health care workers deliver the program including vision tests and fundus imaging with the support of an ophthalmic technician. Retina specialists perform decision-making. Referral plans are interacted through an automated digital program. The screening environment, feedback, ethics and medical legal issues are other main components of the program. Conclusion This study presents the initial concepts and components of a screening program for prevention of blindness in the adult population in Iran. The program has the potential to improve eye health at the community level and may potentially be replicated as a model for similar settings elsewhere.", "gemini": "Mục đích Thiết kế một chương trình sàng lọc để phòng ngừa mù lòa tại cộng đồng ở Iran. Phương pháp Trong nghiên cứu định tính này, các thành phần và đặc tính của chương trình sàng lọc đã được xác định bằng phương pháp nghiên cứu hành động tham gia với các cuộc họp nhóm tập trung (FGM) với các nhà cung cấp dịch vụ chăm sóc sức khỏe và các cơ quan có liên quan. Một phương pháp phân tích nội dung đã được sử dụng để phân tích dữ liệu. Kết quả Tổng cộng, 18 bên liên quan bao gồm sáu bác sĩ nhãn khoa với các chuyên khoa phụ khác nhau đã tham gia vào năm FGM. Chương trình sàng lọc nhằm phát hiện các tình trạng mắt đe dọa thị lực ở những người từ 50 tuổi trở lên. Các nhân viên chăm sóc sức khỏe ban đầu thực hiện chương trình bao gồm các bài kiểm tra thị lực và chụp hình đáy mắt với sự hỗ trợ của một kỹ thuật viên nhãn khoa. Các bác sĩ chuyên khoa võng mạc thực hiện ra quyết định. Các kế hoạch giới thiệu được tương tác thông qua một chương trình kỹ thuật số tự động. Môi trường sàng lọc, phản hồi, đạo đức và các vấn đề pháp lý y tế là những thành phần chính khác của chương trình. Kết luận Nghiên cứu này trình bày các khái niệm và thành phần ban đầu của một chương trình sàng lọc để phòng ngừa mù lòa ở dân số người lớn ở Iran. Chương trình có tiềm năng cải thiện sức khỏe mắt ở cấp cộng đồng và có khả năng được sao chép như một mô hình cho các bối cảnh tương tự ở những nơi khác."} +{"idx": 1900124, "en": "Purpose To report the unusual case of an immunocompetent individual with herpes zoster ophthalmicus who developed central retinal artery occlusion and subsequent neovascular glaucoma. Case Report A 40-year-old, immunocompetent patient was diagnosed with herpes zoster ophthalmicus and central retinal artery occlusion on initial presentation. Subsequently, he developed neovascular glaucoma. Conclusion There are a few case reports of central retinal artery occlusion developing after varicella zoster virus infection. However, a literature search found no reports of neovascular glaucoma following central retinal artery occlusion secondary to varicella zoster virus infection. The present case report indicates that neovascular glaucoma is a possible complication in such a scenario.", "gemini": "Mục đích Báo cáo trường hợp bất thường của một cá nhân có hệ miễn dịch khỏe mạnh bị herpes zoster mắt, người đã phát triển tình trạng tắc động mạch trung tâm võng mạc và sau đó là tăng nhãn áp tân mạch. Báo cáo trường hợp Một bệnh nhân 40 tuổi, có hệ miễn dịch khỏe mạnh đã được chẩn đoán mắc bệnh herpes zoster mắt và tắc động mạch trung tâm võng mạc khi khám ban đầu. Sau đó, anh ta phát triển tăng nhãn áp tân mạch. Kết luận Có một số báo cáo trường hợp về tình trạng tắc động mạch trung tâm võng mạc phát triển sau khi nhiễm vi-rút varicella zoster. Tuy nhiên, tìm kiếm tài liệu không thấy báo cáo nào về bệnh tăng nhãn áp tân mạch sau khi tắc động mạch trung tâm võng mạc thứ phát do nhiễm vi-rút varicella zoster. Báo cáo trường hợp hiện tại chỉ ra rằng tăng nhãn áp tân mạch là một biến chứng có thể xảy ra trong trường hợp như vậy."} +{"idx": 1900125, "en": "Purpose To report visual rehabilitation with a native Pintucci keratoprosthesis (KPro) after a severe ocular surface chemical burn in a male patient. Case Report A 41-year-old man experienced a bilateral severe chemical burn 5 years previously. Earlier penetrating keratoplasty and keratolimbal allografts were unsuccessful in both eyes, and neither of the eyes had vision better than light perception. Both corneas were opaque and conjunctivalized. Because of severe dry eye and total limbal stem cell deficiency, the left eye was considered for a Pintucci-type KPro. In the first stage, the ocular surface was reconstructed with an oral mucus membrane graft, and a KPro was placed under the skin and orbicularis oculi muscle. Three months later, the KPro was removed and implanted in the left eye. During seven months after the KPro implantation, the anatomical position was acceptable, and his best corrected visual acuity was 2/10. Conclusion Bearing in mind the successful results of the native Pintucci KPro in this case of severe acid burn, using this type of keratoprosthesis in patients with total limbal stem cell deficiency and severe dry eye is recommended.", "gemini": "Mục đích Báo cáo phục hồi thị lực bằng giác mạc nhân tạo Pintucci bản địa (KPro) sau khi bị bỏng hóa chất nghiêm trọng ở bề mặt mắt ở một bệnh nhân nam. Báo cáo trường hợp Một người đàn ông 41 tuổi đã bị bỏng hóa chất nghiêm trọng ở cả hai mắt cách đây 5 năm. Ghép giác mạc xuyên thấu trước đó và ghép đồng loại giác mạc và rìa giác mạc không thành công ở cả hai mắt và không mắt nào có thị lực tốt hơn là nhận biết ánh sáng. Cả hai giác mạc đều đục và kết mạc hóa. Do tình trạng khô mắt nghiêm trọng và thiếu tế bào gốc rìa giác mạc hoàn toàn, mắt trái được xem xét để ghép KPro loại Pintucci. Ở giai đoạn đầu, bề mặt nhãn cầu được tái tạo bằng ghép màng nhầy miệng và KPro được đặt dưới da và cơ vòng mi. Ba tháng sau, KPro được tháo ra và cấy vào mắt trái. Trong bảy tháng sau khi cấy ghép KPro, vị trí giải phẫu có thể chấp nhận được và thị lực được hiệu chỉnh tốt nhất của anh ta là 2/10. Kết luận Ghi nhớ kết quả thành công của KPro Pintucci bản địa trong trường hợp bỏng axit nghiêm trọng này, nên sử dụng loại giác mạc nhân tạo này ở những bệnh nhân bị thiếu tế bào gốc rìa giác mạc hoàn toàn và khô mắt nghiêm trọng."} +{"idx": 1900126, "en": "Purpose To present a case of bilateral peripheral ulcerative keratitis (PUK) caused by primary herpes simplex virus-1 (HSV-1) infection resulting in corneal perforation. Case Report A 24-year-old man presented at the eye casualty of our clinic, with a 20-day history of severe pain, redness, photophobia, and tearing in both of his eyes. Slit-lamp examination revealed bilateral superior corneal perforation. A laboratory work-up that included immunological testing for infectious and autoimmune factors showed primary HSV infection. Positive PCR analysis of corneal scrapings for HSV confirmed initial end-organ ocular infection. Because the patient showed progressive HSV-1-related PUK resulting in bilateral superior corneal perforation with iris prolapse, he was prescribed both systemic and topical acyclovir and prednisone. He then underwent bilateral surgical intervention, namely eccentric penetrating keratoplasty in one eye and a two step procedure in the other, whereby two corneal patch grafts and an amniotic membrane transplant were initially used, followed 1 month later by a large diameter penetrating keratoplasty. Conclusion In cases of PUK, differential diagnosis should include infectious and autoimmune diseases. Primary HSV should also be considered as a potential cause of this form of keratitis, which, if left untreated, can lead to devastating outcomes. To our knowledge, this is the first published case of bilateral PUK caused by primary HSV-1 infection.", "gemini": "Mục đích Trình bày một trường hợp viêm loét giác mạc ngoại vi hai bên (PUK) do nhiễm virus herpes simplex-1 (HSV-1) nguyên phát dẫn đến thủng giác mạc. Báo cáo ca bệnh Một nam giới 24 tuổi đến phòng cấp cứu mắt của chúng tôi, có tiền sử 20 ngày đau dữ dội, đỏ, sợ ánh sáng và chảy nước mắt ở cả hai mắt. Khám đèn khe cho thấy thủng giác mạc trên hai bên. Kiểm tra xét nghiệm bao gồm xét nghiệm miễn dịch đối với các yếu tố truyền nhiễm và tự miễn dịch cho thấy nhiễm HSV nguyên phát. Phân tích PCR dương tính của các mẫu cạo giác mạc đối với HSV xác nhận nhiễm trùng cơ quan đích ban đầu ở mắt. Do bệnh nhân biểu hiện PUK liên quan đến HSV-1 tiến triển dẫn đến thủng giác mạc trên hai bên với sa mống mắt, nên anh ta được kê đơn acyclovir và prednisone toàn thân và tại chỗ. Sau đó, anh ta đã trải qua can thiệp phẫu thuật hai bên, cụ thể là ghép giác mạc xuyên tâm lệch tâm ở một bên mắt và một quy trình hai bước ở bên mắt còn lại, trong đó ban đầu sử dụng hai miếng ghép vá giác mạc và một ghép màng ối, sau đó 1 tháng sau là ghép giác mạc xuyên tâm đường kính lớn. Kết luận Trong các trường hợp PUK, chẩn đoán phân biệt nên bao gồm các bệnh truyền nhiễm và tự miễn dịch. HSV nguyên phát cũng nên được coi là một nguyên nhân tiềm ẩn của dạng viêm giác mạc này, nếu không được điều trị có thể dẫn đến hậu quả tàn khốc. Theo chúng tôi biết, đây là trường hợp đầu tiên được công bố về PUK hai bên do nhiễm HSV-1 nguyên phát."} +{"idx": 1900127, "en": "Purpose To present a case of suspected Leber's hereditary optic neuropathy (LHON) with MRI and OCT findings compatible with pseudotumor cerebri responsive to acetazolamide therapy. Case Report A five-year-old boy referred to our clinic with optic atrophy and low vision was originally diagnosed with LHON. Laboratory tests were negative for LHON, while OCT and MRI were consistent with pseudotumor cerebri. Acetazolamide therapy resulted in dramatic improvement of visual acuity. Conclusion Some cases of previously labeled hereditary optic neuropathies with clinical signs of idiopathic intracranial hypertension could respond to intracranial pressure lowering treatment.", "gemini": "Mục đích Trình bày một trường hợp nghi ngờ bệnh thần kinh thị giác di truyền Leber (LHON) với các phát hiện MRI và OCT tương thích với u não giả đáp ứng với liệu pháp acetazolamide. Báo cáo ca bệnh Một bé trai năm tuổi được chuyển đến phòng khám của chúng tôi với tình trạng teo thị thần và thị lực kém ban đầu được chẩn đoán mắc LHON. Các xét nghiệm trong phòng thí nghiệm cho kết quả âm tính với LHON, trong khi OCT và MRI phù hợp với u não giả. Liệu pháp acetazolamide dẫn đến cải thiện đáng kể về thị lực. Kết luận Một số trường hợp bệnh thần kinh thị giác di truyền được dán nhãn trước đây có các dấu hiệu lâm sàng của tăng áp lực nội sọ vô căn có thể đáp ứng với điều trị hạ áp lực nội sọ."} +{"idx": 1900128, "en": "Objective There are some evidence that Vitamin D supplementation may be beneficial for patients with irritable bowel syndrome (IBS). The aim of this study was to evaluate the effects of Vitamin D supplementation on symptoms and quality of life (QOL) in patients with IBS. Methods In a randomized, double-blind, placebo-controlled clinical trial, 116 patients with IBS were supplemented weekly with either a pearl of 50,000 IU Vitamin D or an identical pearl of placebo containing medium chain triglyceride for 6 weeks. Results Mean age of patients was 42.24 ± 12.26, and 40.06 ± 13.37 in Vitamin D and placebo groups, respectively. Dietary intakes were similar between and within groups. Serum concentration of 25-hydroxy Vitamin D increased significantly from 21.10 ± 5.23 to 36.43 ± 12.34 in the Vitamin D group (P < 0.001), while it was not significantly different before and after the trial in placebo group. The IBS symptoms severity scores (SSSs), disease-specific QOL, and total score were evaluated at weeks 0 and 6. IBS-SSS, IBS-QOL, and the total score were improved significantly more in Vitamin D group in comparison to the placebo group (P < 0.05). Conclusions This study indicates that Vitamin D therapy can improve the severity of symptoms and QOL in patients with IBS; however, the long-term effects remained to be elucidated. Trial registration at IRCT: IRCT201402234010N11 IRB Number: 116/3976.", "gemini": "Mục tiêu Có một số bằng chứng cho thấy việc bổ sung Vitamin D có thể có lợi cho những bệnh nhân mắc hội chứng ruột kích thích (IBS). Mục đích của nghiên cứu này là đánh giá tác dụng của việc bổ sung Vitamin D đối với các triệu chứng và chất lượng cuộc sống (QOL) ở những bệnh nhân mắc IBS. Phương pháp Trong một thử nghiệm lâm sàng ngẫu nhiên, mù đôi, có đối chứng giả dược, 116 bệnh nhân mắc IBS được bổ sung hàng tuần một viên ngọc trai chứa 50.000 IU Vitamin D hoặc một viên ngọc trai giả dược giống hệt nhau chứa triglycerid chuỗi trung bình trong 6 tuần. Kết quả Tuổi trung bình của bệnh nhân là 42,24 ± 12,26 và 40,06 ± 13,37 ở nhóm dùng Vitamin D và nhóm dùng giả dược. Lượng ăn vào hàng ngày là tương tự nhau giữa các nhóm và trong nhóm. Nồng độ huyết thanh của Vitamin D 25-hydroxy tăng đáng kể từ 21,10 ± 5,23 lên 36,43 ± 12,34 trong nhóm dùng Vitamin D (P < 0,001), trong khi không có sự khác biệt đáng kể trước và sau thử nghiệm ở nhóm dùng giả dược. Điểm số mức độ nghiêm trọng của các triệu chứng IBS (SSS), QOL đặc hiệu theo bệnh và tổng điểm được đánh giá vào tuần 0 và tuần 6. IBS-SSS, IBS-QOL và tổng điểm được cải thiện đáng kể hơn ở nhóm dùng Vitamin D so với nhóm dùng giả dược (P < 0,05). Kết luận Nghiên cứu này chỉ ra rằng liệu pháp Vitamin D có thể cải thiện mức độ nghiêm trọng của các triệu chứng và QOL ở những bệnh nhân mắc IBS; tuy nhiên, các tác dụng lâu dài vẫn chưa được làm sáng tỏ. Đăng ký thử nghiệm tại IRCT: IRCT201402234010N11 Số IRB: 116/3976."} +{"idx": 1900129, "en": "Purpose To report 2 cases of optic nerve head (ONH) granuloma, with raised serum angiotensin-converting enzyme (ACE) levels not fitting into the existing criteria for ocular sarcoidosis (OS). Case Report Fundus photography, ultrasonography, fluorescein angiography, and optical coherence tomography were performed for both patients. Systemic workup was performed for granulomatous disorders, including sarcoidosis, tuberculosis, and syphilis. Both patients had ONH granulomas and elevated ACE levels, with one of the patients also presenting retinal vasculitis. No other focus of systemic sarcoidosis was localized. Both patients were treated with oral steroids, following which they showed a marked, rapid clinical improvement. Both patients remained stable for at least one year. Conclusion The current accepted criterion for diagnosis of OS may need changes to include such borderline cases due to lack of correlation between clinical and investigative findings.", "gemini": "Mục đích Báo cáo 2 trường hợp u hạt đầu dây thần kinh thị giác (ONH), có nồng độ men chuyển đổi angiotensin huyết thanh (ACE) tăng cao không phù hợp với các tiêu chuẩn hiện có đối với bệnh sarcoid mắt (OS). Báo cáo trường hợp Đã chụp ảnh đáy mắt, siêu âm, chụp mạch huỳnh quang và chụp cắt lớp quang học cho cả hai bệnh nhân. Đã tiến hành kiểm tra toàn thân đối với các rối loạn u hạt, bao gồm bệnh sarcoid, bệnh lao và bệnh giang mai. Cả hai bệnh nhân đều bị u hạt ONH và nồng độ ACE tăng cao, một trong những bệnh nhân cũng biểu hiện viêm mạch võng mạc. Không tìm thấy ổ bệnh sarcoid toàn thân nào khác. Cả hai bệnh nhân đều được điều trị bằng steroid đường uống, sau đó họ cho thấy sự cải thiện lâm sàng nhanh chóng và rõ rệt. Cả hai bệnh nhân đều ổn định trong ít nhất một năm. Kết luận Tiêu chuẩn được chấp nhận hiện tại để chẩn đoán OS có thể cần thay đổi để bao gồm các trường hợp ranh giới như vậy do thiếu mối tương quan giữa các phát hiện lâm sàng và điều tra."} +{"idx": 1900130, "en": "Background The aim of this study was to investigate the effect of hydroethanolic Nigella sativa L. extract on skin wound healing in diabetic male rats. Methods This experimental study was conducted on 49 male Wistar rats weighing 220-250 g divided into 7 groups of 7 each: control (nondiabetic untreated), sham (nondiabetic eucerin-treated), nondiabetic phenytoin (1%)-treated, diabetic untreated, and three diabetic groups treated independently with phenytoin 1%, hydroethanolic N. sativa extracts 20% or 40%. Diabetes was induced with 60 mg/kg streptozosin in one administration. After anesthesia, 2 × 1 cm2 wounds were made on the rats' backs and each group was administered with its own respective treatment until the wounds were healed completely. Tissue specimens were prepared for histological examinations. The areas of the wounds were measured every 3 days. The data were analyzed by ANOVA and Tukey's post-hoc test. Results The mean duration of wound healing was 27 and 24 days for diabetic untreated and diabetic phenytoin-treated groups, respectively. Wounds were healed completely in nondiabetic untreated, sham, and nondiabetic phenytoin-treated groups on days 23, 24, and 21, respectively. The shortest duration of wound healing was seen in diabetic N. sativa extract (40%)-treated group (15 days) followed by diabetic N. sativa (20%)-treated group (18 days). These two groups were found to have the lowest mean wound area during the study with a significant difference from mean wound area in the controls (P < 0.05). Conclusions N. sativa extract significantly promoted wound healing in diabetic rats in comparison with control groups. Although the beneficial mechanism of the promotion of wound healing was not specifically studied, it is believed that the anti-inflammatory and antimicrobial properties of N. sativa would contribute to this enhanced wound healing.", "gemini": "Bối cảnh Mục đích của nghiên cứu này là điều tra tác dụng của chiết xuất Nigella sativa L. hydroethanolic lên quá trình lành vết thương da ở chuột đực bị tiểu đường. Phương pháp Nghiên cứu thực nghiệm này được tiến hành trên 49 con chuột Wistar đực có trọng lượng 220-250 g được chia thành 7 nhóm, mỗi nhóm 7 con: nhóm đối chứng (không tiểu đường, không điều trị), nhóm giả (không tiểu đường, điều trị bằng eucerin), nhóm không tiểu đường điều trị bằng phenytoin (1%), nhóm tiểu đường không điều trị và ba nhóm tiểu đường được điều trị độc lập bằng phenytoin 1%, chiết xuất N. sativa hydroethanolic 20% hoặc 40%. Bệnh tiểu đường được gây ra bằng cách tiêm một liều 60 mg/kg streptozotocin. Sau khi gây mê, người ta tạo ra các vết thương 2 × 1 cm2 trên lưng chuột và mỗi nhóm được dùng phương pháp điều trị riêng cho đến khi vết thương lành hoàn toàn. Chuẩn bị mẫu mô để kiểm tra mô học. Diện tích vết thương được đo 3 ngày một lần. Dữ liệu được phân tích bằng ANOVA và kiểm định Tukey sau thử nghiệm. Kết quả Thời gian trung bình để vết thương lành là 27 và 24 ngày đối với nhóm tiểu đường không điều trị và nhóm tiểu đường điều trị bằng phenytoin. Vết thương lành hoàn toàn ở nhóm đối chứng không tiểu đường, nhóm giả và nhóm không tiểu đường điều trị bằng phenytoin vào các ngày 23, 24 và 21. Thời gian lành vết thương ngắn nhất được ghi nhận ở nhóm tiểu đường được điều trị bằng chiết xuất N. sativa (40%) (15 ngày) tiếp theo là nhóm tiểu đường được điều trị bằng N. sativa (20%) (18 ngày). Hai nhóm này được phát hiện có diện tích vết thương trung bình thấp nhất trong quá trình nghiên cứu với sự khác biệt đáng kể so với diện tích vết thương trung bình ở nhóm đối chứng (P < 0,05). Kết luận Chiết xuất N. sativa thúc đẩy đáng kể quá trình lành vết thương ở chuột tiểu đường so với các nhóm đối chứng. Mặc dù cơ chế có lợi của việc thúc đẩy quá trình lành vết thương không được nghiên cứu cụ thể, nhưng người ta tin rằng các đặc tính chống viêm và kháng khuẩn của N. sativa sẽ góp phần vào quá trình lành vết thương tăng cường này."} +{"idx": 1900131, "en": "Background Vitamin D plays an important role in nervous health and depression. Vitamin D deficiency and anxiety affect diabetic status. The purpose of this study was to determine the effect of vitamin D supplementation on anxiety, depression, and inflammation in diabetic women with anxiety. Methods In this randomized controlled trial, totally 51 women with type 2 diabetes (T2DM) and vitamin D deficiency were randomly allocated to receive one oral pearl of 50,000 IU vitamin D3 (26 women) or a placebo (25 women) fortnightly for 16 weeks. Anthropometric indices, sun exposure, dietary intake, depression, anxiety, and stress scores and biochemical biomarkers including high sensitivity C-reactive protein (hs-CRP) and interleukin-10 (IL-10) were measured at the baseline and after 16-week supplementation. Results Mean ± SD age of participant was 47.43 ± 9.57 years old. Baseline values were not different between the groups. Anxiety score changes were significantly lower in vitamin D group than the controls (P = 0.001). Within group comparison indicated that depression in supplement group with lower vitamin D levels was significantly reduced. Serum hs-CRP reduced (P = 0.01), while IL-10 concentrations increased (P = 0.04) in the intervention group. Conclusions Vitamin D supplementation can improve mood status and anti-inflammatory biomarkers in female diabetics with anxiety and vitamin D deficiency.", "gemini": "Bối cảnh Vitamin D đóng vai trò quan trọng đối với sức khỏe thần kinh và chứng trầm cảm. Thiếu vitamin D và lo lắng ảnh hưởng đến tình trạng bệnh tiểu đường. Mục đích của nghiên cứu này là xác định tác dụng của việc bổ sung vitamin D đối với chứng lo lắng, trầm cảm và tình trạng viêm ở phụ nữ tiểu đường có lo lắng. Phương pháp Trong thử nghiệm đối chứng ngẫu nhiên này, tổng cộng 51 phụ nữ mắc bệnh tiểu đường loại 2 (T2DM) và thiếu vitamin D đã được phân bổ ngẫu nhiên để dùng một viên ngọc trai uống 50.000 IU vitamin D3 (26 phụ nữ) hoặc giả dược (25 phụ nữ) hai tuần một lần trong 16 tuần. Các chỉ số nhân trắc học, phơi nắng, lượng ăn vào, điểm trầm cảm, lo lắng và căng thẳng và các dấu sinh học sinh hóa bao gồm protein phản ứng C độ nhạy cao (hs-CRP) và interleukin-10 (IL-10) được đo ở thời điểm ban đầu và sau 16 tuần bổ sung. Kết quả Tuổi trung bình ± SD của người tham gia là 47,43 ± 9,57 tuổi. Các giá trị ban đầu không khác nhau giữa các nhóm. Sự thay đổi điểm lo lắng thấp hơn đáng kể ở nhóm dùng vitamin D so với nhóm đối chứng (P = 0,001). So sánh trong nhóm cho thấy trầm cảm ở nhóm bổ sung có mức vitamin D thấp hơn giảm đáng kể. Nồng độ hs-CRP trong huyết thanh giảm (P = 0,01), trong khi nồng độ IL-10 tăng (P = 0,04) ở nhóm can thiệp. Kết luận Bổ sung vitamin D có thể cải thiện tình trạng tâm trạng và các dấu sinh học chống viêm ở phụ nữ tiểu đường có lo lắng và thiếu vitamin D."} +{"idx": 1900132, "en": "Body image is the perception of individuals of their own body and it can be affected by many variables such as culture, social pressure, and media, but it is not limited to these factors. Body image disturbances are important because they lead to severe physical or psychological health problems. Development of body image dissatisfaction in Eastern communities has been increased in the past years. Despite many studies in Asian societies, In Iran, studies are scattered and limited. Our goal is a better understanding of this matter in Iran. A review of scientific literature about Body Image and Iran was conducted inPubMed, Web of Science, Science Direct, Scopus, and ProQuest, also in Persian scientific databases such as Scientific Information Database (SID) and Thematic Guide to Iranian Publications (MAGIRAN). This search has been conducted in January 2018. Out of 389 articles, 44 of them were selected. In these articles, study period, sample characteristics, type of study, contributing variables, type of statistical analysis, and the main result of each article were extracted. Despite using different methods for detection of body dissatisfaction, results showed that this problem has a high prevalence in Iranian population. Contributing factors were also similar to the world findings. Review of studies regarding body dissatisfaction in Iran, revealed a high prevalence of this problem. It seems there is a need for interventional programs to prevent the negative consequences of body dissatisfaction, especially in teenagers and young people. Future studies with scientific or longitudinal design, which lead to better results, are recommended.", "gemini": "Hình ảnh cơ thể là nhận thức của cá nhân về cơ thể của chính họ và nó có thể bị ảnh hưởng bởi nhiều biến số như văn hóa, áp lực xã hội và phương tiện truyền thông, nhưng không chỉ giới hạn ở những yếu tố này. Rối loạn hình ảnh cơ thể rất quan trọng vì chúng dẫn đến các vấn đề sức khỏe thể chất hoặc tâm lý nghiêm trọng. Sự phát triển của sự không hài lòng về hình ảnh cơ thể ở các cộng đồng phương Đông đã gia tăng trong những năm gần đây. Mặc dù có nhiều nghiên cứu trong các xã hội châu Á, nhưng ở Iran, các nghiên cứu còn rải rác và hạn chế. Mục tiêu của chúng tôi là hiểu rõ hơn về vấn đề này ở Iran. Đã tiến hành xem xét các tài liệu khoa học về Hình ảnh cơ thể và Iran trên PubMed, Web of Science, Science Direct, Scopus và ProQuest, cũng như trong các cơ sở dữ liệu khoa học tiếng Ba Tư như Cơ sở dữ liệu thông tin khoa học (SID) và Hướng dẫn chuyên đề về ấn phẩm của Iran (MAGIRAN). Tìm kiếm này đã được thực hiện vào tháng 1 năm 2018. Trong số 389 bài báo, 44 bài đã được chọn. Trong các bài báo này, thời gian nghiên cứu, đặc điểm mẫu, loại nghiên cứu, các biến đóng góp, loại phân tích thống kê và kết quả chính của từng bài báo đã được trích xuất. Mặc dù sử dụng các phương pháp khác nhau để phát hiện sự không hài lòng về cơ thể, nhưng kết quả cho thấy vấn đề này có tỷ lệ lưu hành cao trong dân số Iran. Các yếu tố góp phần cũng tương tự như các phát hiện trên thế giới. Đánh giá các nghiên cứu về sự không hài lòng về cơ thể ở Iran cho thấy tỷ lệ lưu hành cao của vấn đề này. Có vẻ như cần có các chương trình can thiệp để ngăn ngừa hậu quả tiêu cực của sự không hài lòng về cơ thể, đặc biệt là ở thanh thiếu niên và những người trẻ tuổi. Các nghiên cứu trong tương lai với thiết kế khoa học hoặc theo chiều dọc, dẫn đến kết quả tốt hơn, được khuyến nghị."} +{"idx": 1900133, "en": "Our aims to examine the factors influencing self-care for the prevention and control of preeclampsia in high-risk women. The current study is a review where the researcher browsed the available databases such as PubMed, Cochrane, Medline, Google Scholar, Medscape, and relevant research published between 1980 and 2016 were studied. To search for articles, relevant Medical Subject Heading keywords were first determined (Self-care, preeclampsia, prevention.) A total of 350 related articles were first selected, and the findings of 70 were used to compile the present article. The results of the study were classified under two general categories, including (1). Counseling and screening strategies and (2) self-care strategies for the prevention and control of preeclampsia in high-risk women. Screening women at risk for preeclampsia include measures such as measuring their blood pressure, checking for signs of depression, testing for thrombosis, taking a history of preeclampsia, providing preconception counseling about the appropriate age, time of pregnancy, and encouraging weight loss in obese women. This review showed a positive relationship between knowledge about self-care for preeclampsia and its control. The factors influencing preeclampsia self-care include making lifestyle changes, having a healthy diet, learning stress management, performing exercise and physical activities, taking antioxidants, dietary supplements, and calcium and adherence to aspirin and heparin regimens. There is a positive relationship between preconception counseling, screening women at risk for preeclampsia, self-care for the prevention, and control of preeclampsia. (1) Tweetable abstract self-care in high-risk women is strongly associated with prevention and control of preeclampsia.", "gemini": "Mục đích của chúng tôi là xem xét các yếu tố ảnh hưởng đến việc tự chăm sóc để phòng ngừa và kiểm soát tiền sản giật ở phụ nữ có nguy cơ cao. Nghiên cứu hiện tại là một đánh giá trong đó các nhà nghiên cứu duyệt các cơ sở dữ liệu có sẵn như PubMed, Cochrane, Medline, Google Scholar, Medscape và các nghiên cứu có liên quan được công bố từ năm 1980 đến năm 2016 đã được nghiên cứu. Để tìm kiếm các bài báo, trước tiên, các từ khóa tiêu đề chủ đề y khoa có liên quan đã được xác định (Tự chăm sóc, tiền sản giật, phòng ngừa). Tổng cộng 350 bài báo liên quan đã được chọn đầu tiên và các phát hiện của 70 bài đã được sử dụng để biên soạn bài báo hiện tại. Kết quả nghiên cứu được phân loại thành hai loại chung, bao gồm (1). Chiến lược tư vấn và sàng lọc và (2) các chiến lược tự chăm sóc để phòng ngừa và kiểm soát tiền sản giật ở phụ nữ có nguy cơ cao. Sàng lọc những phụ nữ có nguy cơ tiền sản giật bao gồm các biện pháp như đo huyết áp, kiểm tra các dấu hiệu trầm cảm, xét nghiệm huyết khối, tiền sử tiền sản giật, cung cấp tư vấn trước khi thụ thai về độ tuổi thích hợp, thời gian mang thai và khuyến khích giảm cân ở những phụ nữ béo phì. Đánh giá này cho thấy mối quan hệ tích cực giữa kiến thức về tự chăm sóc đối với tiền sản giật và việc kiểm soát bệnh. Các yếu tố ảnh hưởng đến việc tự chăm sóc tiền sản giật bao gồm thay đổi lối sống, có chế độ ăn uống lành mạnh, học cách quản lý căng thẳng, thực hiện các bài tập và hoạt động thể chất, dùng chất chống oxy hóa, thực phẩm bổ sung và canxi, tuân thủ chế độ dùng aspirin và heparin. Có mối quan hệ tích cực giữa tư vấn trước khi thụ thai, sàng lọc những phụ nữ có nguy cơ tiền sản giật, tự chăm sóc để phòng ngừa và kiểm soát tiền sản giật. (1) Tóm tắt có thể tweet được về việc tự chăm sóc ở những phụ nữ có nguy cơ cao có liên quan chặt chẽ đến việc phòng ngừa và kiểm soát tiền sản giật."} +{"idx": 1900134, "en": "Background Birth asphyxia is considered as one of the biggest challenges faced by perinatal care experts. According to the WHO, in 2005, one-fourth of infant mortality cases occurred due to birth asphyxia. Methods This study is a retrospective study done on the newborn population with gestational ages of 36 weeks or higher during the years 2013, 2014, and 2015 to find the relationship between the number of birth asphyxia cases and the years. As a secondary objective, the relationship between the mode of delivery and the cases of birth asphyxia were investigated using logistic regression test. The inclusion criteria consisted of three cases, namely, gestational age of 36 weeks or higher, 5 min Apgar of <5, and umbilical cord pH of <7.1. The exclusion criteria involved hydrops fetalis, cyanotic heart disease, chromosomal abnormality, and congenital infections. Results From the two independent variables of the study, only the variable \"year\" showed a significant difference between the years 2013 and 2015 (P < 0.01). The delivery mode did not have any statistically significant influence on the newborns' affliction with birth asphyxia (P = 0.993). Conclusions According to the results, there is a significant difference between the rate of birth asphyxia in Iran and its global rate in 2015, and regarding its multiple growth from 2013 to 2015, conducting a more comprehensive and extensive research on birth asphyxia risk factors at a delivery time seems justified and inevitable.", "gemini": "Bối cảnh Ngạt thở khi sinh được coi là một trong những thách thức lớn nhất mà các chuyên gia chăm sóc trẻ sơ sinh phải đối mặt. Theo WHO, vào năm 2005, một phần tư các trường hợp tử vong ở trẻ sơ sinh xảy ra do ngạt thở khi sinh. Phương pháp Nghiên cứu này là một nghiên cứu hồi cứu được thực hiện trên quần thể trẻ sơ sinh có tuổi thai từ 36 tuần trở lên trong các năm 2013, 2014 và 2015 để tìm mối quan hệ giữa số ca ngạt thở khi sinh và các năm. Mục tiêu thứ cấp là mối quan hệ giữa phương thức sinh và các trường hợp ngạt thở khi sinh đã được điều tra bằng cách sử dụng kiểm tra hồi quy logistic. Các tiêu chuẩn đưa vào bao gồm ba trường hợp, cụ thể là tuổi thai từ 36 tuần trở lên, điểm Apgar 5 phút <5 và pH dây rốn <7,1. Các tiêu chuẩn loại trừ liên quan đến phù thai, bệnh tim tím bẩm sinh, bất thường nhiễm sắc thể và nhiễm trùng bẩm sinh. Kết quả Trong số hai biến độc lập của nghiên cứu, chỉ có biến \"năm\" cho thấy sự khác biệt đáng kể giữa các năm 2013 và 2015 (P < 0,01). Phương thức sinh không có bất kỳ ảnh hưởng thống kê đáng kể nào đến tình trạng ngạt thở khi sinh của trẻ sơ sinh (P = 0,993). Kết luận Theo kết quả, có sự khác biệt đáng kể giữa tỷ lệ ngạt thở khi sinh ở Iran và tỷ lệ toàn cầu vào năm 2015, và xét đến sự gia tăng nhiều lần từ năm 2013 đến năm 2015, việc tiến hành một nghiên cứu toàn diện và sâu rộng hơn về các yếu tố nguy cơ ngạt thở khi sinh tại thời điểm sinh dường như là hợp lý và không thể tránh khỏi."} +{"idx": 1900135, "en": "Lower extremity ulcers represent the most ominous, feared, and costly complications of diabetes mellitus. The aim of this review is to highlight the role of daily life physical activities (PAs) and continuous movement monitoring (CMM) in the prevention of foot ulcers. Peripheral neuropathy and peripheral vascular disease are the main causes of foot ulceration and contribute, in turn, to the development of additional risk factors such as foot deformities and/or joint and muscular alterations. Moreover, a deficit of balance, posture abnormalities, followed by gait alterations, increases the risk of ulceration. PA can play a key role in the management of patients with diabetes and in the prevention of ulcers; however, even if it has been reported that some of these risk factors significantly improve after a few weeks of exercise therapy (ET), the real preventive role of ET has not yet been demonstrated. These uncertain results can occur due to some limitations in the management of the same relationship between PA and diabetic foot prevention. Technological advances during the last years enable timely management of overall daily PA. The use of these modern technologies and devices allows CMM assessment and description of daily PA even in the long term. The data collected from these devices can be used to properly manage patients' PA and thus contribute to the prevention of foot ulcers.", "gemini": "Loét chi dưới là biến chứng đáng sợ nhất, tốn kém nhất và đáng lo ngại nhất của bệnh đái tháo đường. Mục đích của bài tổng quan này là làm nổi bật vai trò của các hoạt động thể chất trong cuộc sống hàng ngày (PA) và theo dõi chuyển động liên tục (CMM) trong việc phòng ngừa loét bàn chân. Bệnh thần kinh ngoại biên và bệnh mạch ngoại biên là nguyên nhân chính gây loét bàn chân và ngược lại, góp phần làm phát triển các yếu tố nguy cơ khác như biến dạng bàn chân và/hoặc thay đổi khớp và cơ. Hơn nữa, tình trạng mất cân bằng, bất thường về tư thế, tiếp theo là thay đổi dáng đi làm tăng nguy cơ loét. PA có thể đóng vai trò chính trong việc quản lý bệnh nhân mắc bệnh tiểu đường và phòng ngừa loét; tuy nhiên, ngay cả khi có báo cáo cho rằng một số yếu tố nguy cơ này cải thiện đáng kể sau một vài tuần tập vật lý trị liệu (ET), vai trò phòng ngừa thực sự của ET vẫn chưa được chứng minh. Những kết quả không chắc chắn này có thể xảy ra do một số hạn chế trong việc quản lý mối quan hệ giữa PA và phòng ngừa bàn chân tiểu đường. Những tiến bộ về công nghệ trong những năm gần đây cho phép quản lý kịp thời PA hàng ngày nói chung. Việc sử dụng các công nghệ và thiết bị hiện đại này cho phép đánh giá CMM và mô tả PA hàng ngày ngay cả trong thời gian dài. Dữ liệu thu thập được từ các thiết bị này có thể được sử dụng để quản lý đúng cách PA của bệnh nhân và do đó góp phần phòng ngừa loét bàn chân."} +{"idx": 1900136, "en": "Background Chorionic villus sampling (CVS) and amniocentesis are two methods for prenatal diagnosis. The goal of this study was to compare amniocentesis and CVS-related complications in a large sample of Iranian women. Methods Medical records of 1624 women who underwent amniocentesis or CVS due to medical indications between 2008 and 2016 were reviewed. Data regarding age, gravidity, parity, gestational age, type of procedure, neonatal weight (and percentile), trisomia, abortion, intrauterine growth restriction (IUGR), severe IUGR, preeclampsia, and gestational hypertension were recorded. Results Finally, 1215 cases were evaluated. Mean maternal age, gravidity, and gestational age were significantly different between two groups. Preeclampsia, gestational hypertension, IUGR, severe IUGR, and intrauterine fetal death were not significantly different between two groups. Trisomy 18 and 21 were common in cases underwent amniocentesis. Conclusions Women who underwent CVS are not at higher risk for developing hypertensive disorders than women underwent amniocentesis.", "gemini": "Lý lịch Lấy mẫu nhung mao màng đệm (CVS) và chọc ối là hai phương pháp chẩn đoán trước sinh. Mục tiêu của nghiên cứu này là so sánh các biến chứng liên quan đến chọc ối và CVS trong một nhóm phụ nữ Iran đông đảo. Phương pháp Hồ sơ bệnh án của 1624 phụ nữ đã trải qua chọc ối hoặc CVS do các chỉ định y tế từ năm 2008 đến năm 2016 đã được xem xét. Dữ liệu về tuổi, số lần mang thai, số lần sinh, tuổi thai, loại thủ thuật, cân nặng sơ sinh (và phần trăm), trisomia, phá thai, chậm phát triển trong tử cung (IUGR), IUGR nghiêm trọng, tiền sản giật và tăng huyết áp thai kỳ đã được ghi lại. Kết quả Cuối cùng, 1215 trường hợp đã được đánh giá. Tuổi trung bình của bà mẹ, số lần mang thai và tuổi thai khác nhau đáng kể giữa hai nhóm. Tiền sản giật, tăng huyết áp thai kỳ, IUGR, IUGR nghiêm trọng và tử vong của thai nhi trong tử cung không khác nhau đáng kể giữa hai nhóm. Trisomy 18 và 21 phổ biến ở những trường hợp trải qua chọc ối. Kết luận Phụ nữ trải qua CVS không có nguy cơ mắc các rối loạn tăng huyết áp cao hơn những phụ nữ trải qua chọc ối."} +{"idx": 1900137, "en": "Nuclear anomalies of different types appear in cells in response to the action of ionizing radiation after the passage of the first mitotic division. In this article, we present the results of the study of the frequency of occurrence of three types of nuclear anomalies (\"tailed\" nuclei, nucleoplasmic bridges, and dumbbell-shaped nuclei) in vitro in human lymphocytes cultured with cytochalasin B when exposed to X-rays at doses of 0.0, 0.1, 0.2, 0.4, 0.5, 0.7, 1.0, 1.5, and 2.0 Gy. To stop the cell cycle of cultured lymphocytes after the first mitotic division, a cytokinesis block was performed using cytochalasin B. Dose-dependent curves of the occurrence of lymphocytes containing \"tailed\" nuclei, nucleoplasmic bridges, or dumbbell-shaped nuclei after irradiation have been constructed. At the same time, frequencies of occurrence of chromosomal aberrations (dicentric and ring chromosomes) in the culture of lymphocytes exposed to the same radiation doses were studied. Comparison of the frequencies of occurrence of dicentric and ring chromosomes with frequencies of occurrence of nuclear anomalies allows us to conclude that these nuclear anomalies are formed as a result of chromosomal aberrations arising in lymphocytes under the action of ionizing radiation. More than that, most of the chromosomal aberrations are converted into dumbbell-shaped nuclei in vitro in the culture of lymphocytes in the cytochalasin block.", "gemini": "Các bất thường về nhân tế bào với các loại khác nhau xuất hiện trong tế bào để đáp ứng với tác động của bức xạ ion hóa sau khi trải qua lần phân chia nguyên phân đầu tiên. Trong bài viết này, chúng tôi trình bày kết quả nghiên cứu về tần suất xuất hiện của ba loại bất thường về nhân (\"đuôi\" nhân, cầu nối nhân và nhân hình quả tạ) trong ống nghiệm ở tế bào lympho người được nuôi cấy với cytochalasin B khi tiếp xúc với tia X ở liều 0,0, 0,1, 0,2, 0,4, 0,5, 0,7, 1,0, 1,5 và 2,0 Gy. Để ngăn chặn chu kỳ tế bào của tế bào lympho được nuôi cấy sau lần phân chia nguyên phân đầu tiên, khối phân bào đã được thực hiện bằng cách sử dụng cytochalasin B. Các đường cong phụ thuộc vào liều của sự xuất hiện của tế bào lympho chứa nhân \"đuôi\", cầu nối nhân hoặc nhân hình quả tạ sau khi chiếu xạ đã được xây dựng. Đồng thời, tần suất xuất hiện các quang sai nhiễm sắc thể (nhiễm sắc thể hai tâm và nhiễm sắc thể vòng) trong nuôi cấy tế bào lympho tiếp xúc với cùng liều bức xạ đã được nghiên cứu. So sánh tần suất xuất hiện nhiễm sắc thể hai tâm và nhiễm sắc thể vòng với tần suất xuất hiện các bất thường về nhân cho phép chúng ta kết luận rằng các bất thường về nhân này được hình thành do quang sai nhiễm sắc thể phát sinh ở tế bào lympho dưới tác động của bức xạ ion hóa. Hơn thế nữa, hầu hết các quang sai nhiễm sắc thể được chuyển đổi thành nhân hình quả tạ trong ống nghiệm trong nuôi cấy tế bào lympho trong khối cytochalasin."} +{"idx": 1900138, "en": "Naturally occurring radiation can be found all around us and account for most of the radiation received by human beings each year. Indonesia has a region with high-dose natural radiation located in the suburb of West Sulawesi province with a dose rate up to 2800 nSv/h; however, its impact was not fully understood. The aim of this study was to evaluate the radiosensitivity of 12 peripheral blood lymphocytes of inhabitant from high background radiation area (HBRA) and 10 from normal background radiation area (NBRA) based on cytokinesis-block micronucleus (CBMN) assay after challenged with 1.5 Gy of gamma ray. The analysis of CBMN was done according to standard procedure as per IAEA guidelines, and frequency of binucleate (mitotic) cells with micronuclei (MN) was scored in around 2000 binucleate lymphocytes cells per culture in microscopic analysis. Mean MN frequency for HBRA was lower than that of NBRA (0.121 vs. 0.189) after irradiation, indicating an adaptive response in HBRA group that resulted in less radiosensitivity; however, there was no statistically significant different (P > 0.05) between these two groups. The MN number was higher in women compared to men for both HBRA (0.15 vs. 0.09) and NBRA (0.216 vs. 0.147) groups. Besides, there was no statistically significant difference (P > 0.05) in Nuclear Division Index (NDI), as measured in 500 metaphase cells with published formula, between HBRA and NBRA samples (1.24 vs. 1.21). The lower MN frequency prompts us to conclude that there is an adaptive response in the lymphocytes of inhabitants as an indicator of lower radiosensitivity to the high natural radiation exposure. Further studies using large number of samples are required to obtain more comprehensive conclusion along with the assessment of other types of radiosensitivity-related biomarkers.", "gemini": "Có thể tìm thấy bức xạ tự nhiên ở khắp mọi nơi xung quanh chúng ta và chiếm phần lớn bức xạ mà con người tiếp nhận mỗi năm. Indonesia có một khu vực có bức xạ tự nhiên liều cao nằm ở vùng ngoại ô của tỉnh Tây Sulawesi với tốc độ liều lên tới 2800 nSv/h; tuy nhiên, tác động của nó vẫn chưa được hiểu đầy đủ. Mục đích của nghiên cứu này là đánh giá độ nhạy bức xạ của 12 tế bào lympho máu ngoại vi của cư dân từ khu vực có bức xạ nền cao (HBRA) và 10 tế bào từ khu vực có bức xạ nền bình thường (NBRA) dựa trên xét nghiệm vi nhân tế bào chặn phân bào (CBMN) sau khi thử thách với 1,5 Gy tia gamma. Phân tích CBMN được thực hiện theo quy trình chuẩn theo hướng dẫn của IAEA và tần số các tế bào hai nhân (phân bào) có vi nhân (MN) được tính điểm trong khoảng 2000 tế bào lympho hai nhân trên mỗi nuôi cấy trong phân tích kính hiển vi. Tần số MN trung bình đối với HBRA thấp hơn so với NBRA (0,121 so với 0,189) sau khi chiếu xạ, cho thấy phản ứng thích ứng trong nhóm HBRA dẫn đến độ nhạy bức xạ thấp hơn; tuy nhiên, không có sự khác biệt đáng kể về mặt thống kê (P > 0,05) giữa hai nhóm này. Số MN cao hơn ở phụ nữ so với nam giới đối với cả nhóm HBRA (0,15 so với 0,09) và NBRA (0,216 so với 0,147). Bên cạnh đó, không có sự khác biệt đáng kể về mặt thống kê (P > 0,05) về Chỉ số phân chia nhân (NDI), được đo trong 500 tế bào kỳ giữa với công thức đã công bố, giữa các mẫu HBRA và NBRA (1,24 so với 1,21). Tần số MN thấp hơn thúc đẩy chúng tôi kết luận rằng có phản ứng thích ứng trong các tế bào lympho của cư dân như một chỉ báo về độ nhạy bức xạ thấp hơn đối với phơi nhiễm bức xạ tự nhiên cao. Cần có thêm các nghiên cứu sử dụng số lượng lớn mẫu để có được kết luận toàn diện hơn cùng với đánh giá các loại sinh học chỉ điểm liên quan đến độ nhạy bức xạ khác."} +{"idx": 1900139, "en": "Background Endothelial cells (EC) are sensitive to changes in the microenvironment, including hypoxia and ischemia. Disruption of the microtubular network has been reported in cases of ischemia. However, the signaling pathways involved in hypoxia-induced microtubular disruption are unknown. The purpose of this study was to investigate the molecular mechanisms involved in hypoxia-induced microtubular disassembly in human umbilical vein endothelial cells (HUVECs). Results HUVECs were cultured under normoxic or hypoxic conditions and pretreated with or without colchicine or paclitaxel. The MTT assay, Transwell assay, trans-endothelial permeability assay, and 5-bromo-2'-deoxy-uridine staining were used to test the survival rate, migration, permeability, and proliferation of cells, respectively. Transmission electron microscopy and phalloidin staining were used to observe the microstructure and polymerization of microtubules. The results show that the functions of HUVECs and the microtubular structure were destroyed by hypoxia, but were protected by paclitaxel and a reactive oxygen species (ROS) inhibitor. We further used western blot, a luciferase assay, and co-immunoprecipitation to describe a non-transcription-independent mechanism for PI3K activation-inhibited microtubular stability mediated by Stathmin1, a PI3K interactor that functions in microtubule depolymerization. Finally, we determined that hypoxia and ROS blocked the interaction between PI3K and Stathmin1 to activate disassembly of microtubules. Conclusion Thus, our data demonstrate that hypoxia induced the production of ROS and damaged EC function by destroying the microtubular structure through the PI3K/stathmin1 pathway.", "gemini": "Bối cảnh Các tế bào nội mô (EC) nhạy cảm với những thay đổi trong vi môi trường, bao gồm cả tình trạng thiếu oxy và thiếu máu cục bộ. Sự phá vỡ mạng lưới vi ống đã được báo cáo trong các trường hợp thiếu máu cục bộ. Tuy nhiên, các con đường truyền tín hiệu liên quan đến sự phá vỡ vi ống do thiếu oxy gây ra vẫn chưa được biết rõ. Mục đích của nghiên cứu này là điều tra các cơ chế phân tử liên quan đến quá trình tháo rời vi ống do thiếu oxy trong các tế bào nội mô tĩnh mạch rốn của người (HUVEC). Kết quả Các tế bào HUVEC được nuôi cấy trong điều kiện thiếu oxy hoặc thiếu oxy và được xử lý trước bằng colchicine hoặc paclitaxel hoặc không. Xét nghiệm MTT, xét nghiệm Transwell, xét nghiệm tính thấm xuyên nội mô và nhuộm 5-bromo-2'-deoxy-uridine được sử dụng để kiểm tra tỷ lệ sống sót, di chuyển, tính thấm và tăng sinh của tế bào. Kính hiển vi điện tử truyền qua và nhuộm phalloidin được sử dụng để quan sát cấu trúc vi mô và quá trình trùng hợp của vi ống. Kết quả cho thấy các chức năng của HUVEC và cấu trúc vi ống bị phá hủy do thiếu oxy, nhưng được bảo vệ bởi paclitaxel và chất ức chế các loài oxy phản ứng (ROS). Chúng tôi đã sử dụng thêm phương pháp western blot, xét nghiệm luciferase và đồng kết tủa miễn dịch để mô tả cơ chế không phụ thuộc vào phiên mã đối với sự ổn định vi ống bị ức chế hoạt hóa PI3K do Stathmin1, một chất tương tác PI3K có chức năng trong quá trình khử trùng vi ống. Cuối cùng, chúng tôi xác định rằng tình trạng thiếu oxy và ROS đã chặn sự tương tác giữa PI3K và Stathmin1 để kích hoạt quá trình tháo rời vi ống. Kết luận Do đó, dữ liệu của chúng tôi chứng minh rằng tình trạng thiếu oxy đã gây ra sự sản xuất ROS và làm hỏng chức năng EC bằng cách phá hủy cấu trúc vi ống thông qua con đường PI3K/stathmin1."} +{"idx": 1900140, "en": "Background Skin prick tests (SPTs) are essential for the diagnosis of IgE-mediated allergy and are influenced by extract quality, biological potency and concentration of allergen. Methods In this open multicentre study 431 patients, aged 18-64 years were enrolled. Patients had a history of IgE-mediated allergy and a sensitisation (previous positive SPT of any manufacturer) against at least one of the investigated allergens: 6-grass pollen, house dust mite, birch and mugwort pollen. In our study, these allergens were tested in five concentrations each. To establish the optimal trade-off between sensitivity and specificity, the area under the receiver operating characteristic (ROC) curve was estimated by comparing the outcome of the SPT with three methods referred to as 'reference methods' (specific IgE, clinical case history and a previous SPT). Results For all allergens and reference methods, the area under the ROC curves were highly significant (p < 0.001). Specific IgE reference method resulted in the largest area under the curve (AUC) for all allergens (0.80-0.90) followed by previous SPT (0.70-0.87) and case history (0.65-0.74). Sensitivity of SPT increased with increasing concentration and specificity decreased. For all allergens, compared to specific IgE, the highest sensitivity (specificity at least 80%) was observed for the SPT solution of 50,000 Standardised Units (SU)/mL (grass pollen, birch pollen, house dust mite and mugwort). Conclusion In this study, with a large number of patients, it was demonstrated that clinical case history, previous SPT and specific IgE measurement could all be used as reference methods for the assessment of sensitivity/specificity of SPT solutions. The comparison of SPT with specific IgE resulted in the largest AUC. The highest sensitivity was observed for the SPT solution of 50,000 SU/mL.Trial registration EudraCT: 2006-005304-14.", "gemini": "Bối cảnh Xét nghiệm chích da (SPT) rất cần thiết để chẩn đoán dị ứng do IgE và chịu ảnh hưởng của chất lượng chiết xuất, hoạt lực sinh học và nồng độ chất gây dị ứng. Phương pháp Trong nghiên cứu đa trung tâm mở này, 431 bệnh nhân, tuổi từ 18-64 đã được tuyển dụng. Bệnh nhân có tiền sử dị ứng do IgE và nhạy cảm (SPT dương tính trước đó của bất kỳ nhà sản xuất nào) với ít nhất một trong các chất gây dị ứng được nghiên cứu: 6 phấn cỏ, mạt bụi nhà, phấn cây bạch dương và cây ngải cứu. Trong nghiên cứu của chúng tôi, những chất gây dị ứng này được thử nghiệm ở năm nồng độ khác nhau. Để thiết lập sự đánh đổi tối ưu giữa độ nhạy và độ đặc hiệu, diện tích dưới đường cong đặc tính hoạt động của máy thu (ROC) được ước tính bằng cách so sánh kết quả của SPT với ba phương pháp được gọi là 'phương pháp tham chiếu' (IgE đặc hiệu, tiền sử bệnh lâm sàng và SPT trước đó). Kết quả Đối với tất cả các chất gây dị ứng và phương pháp tham chiếu, diện tích dưới đường cong ROC có ý nghĩa thống kê cao (p < 0,001). Phương pháp tham chiếu IgE đặc hiệu cho kết quả diện tích dưới đường cong (AUC) lớn nhất đối với tất cả các chất gây dị ứng (0,80-0,90) tiếp theo là SPT trước đó (0,70-0,87) và tiền sử bệnh (0,65-0,74). Độ nhạy của SPT tăng theo nồng độ tăng và độ đặc hiệu giảm. Đối với tất cả các chất gây dị ứng, so với IgE đặc hiệu, độ nhạy cao nhất (độ đặc hiệu ít nhất 80%) được quan sát thấy đối với dung dịch SPT 50.000 Đơn vị chuẩn (SU)/mL (phấn cỏ, phấn cây bạch dương, mạt bụi nhà và cây ngải cứu). Kết luận Trong nghiên cứu này, với số lượng lớn bệnh nhân, đã chứng minh rằng tiền sử bệnh lâm sàng, SPT trước đó và đo IgE đặc hiệu đều có thể được sử dụng làm phương pháp tham chiếu để đánh giá độ nhạy/độ đặc hiệu của các dung dịch SPT. Việc so sánh SPT với IgE đặc hiệu cho kết quả AUC lớn nhất. Độ nhạy cao nhất được quan sát thấy đối với dung dịch SPT 50.000 SU/mL. Đăng ký thử nghiệm EudraCT: 2006-005304-14."} +{"idx": 1900141, "en": "Background While there is a broad spectrum of practice within chiropractic two sub-types can be identified, those who focus on musculoskeletal problems and those who treat also non-musculoskeletal problems. The latter group may adhere to the old conservative 'subluxation' model. The main goal of this study is to determine if chiropractic students with such conservative opinions are likely to have a different approach to determine contra-indications, non-indications and indications to chiropractic treatment versus those without such opinions. Method An anonymous and voluntary survey on 3rd to 6th year French chiropractic students was conducted between November 2017 and January 2018. Level of chiropractic conservatism (10 items) and the ability to determine contra-indications (2 cases), non-indications (4 cases) and indications (3 cases) were evaluated through a questionnaire. Answers to these cases were dichotomized into 'appropriate' and 'inappropriate' answers, as defined by previous research teams and the present team. The level of conservatism was classified into four groups, 'group 4' corresponding to the highest score. Descriptive data are provided, and bi- and multivariate analyses were performed through logistic regression to test the associations between the level of conservatism and the ability to determine the suitability of chiropractic treatment. Results In all, 359 of 536 (67%) students responded to the questionnaire. They generally recognized a number of contra-indications and indications to treatment. However, they found it more difficult to identify non-indications. The more conservative students were more likely to intend to treat their patients, even if this was irrelevant (non-indications). For example, those who were most conservative (group 4) were much more willing than those in group 1 to treat 'chiropractically' a 5-year-old child with no history of back pain or disease to prevent future back pain (OR = 14.7) and also to prevent non-musculoskeletal disease (OR = 22). Conclusion It is concerning that students who adhere to the subluxation model are prepared to 'operationalize' their conservative opinions in their future scope of practice; apparently willing to treat asymptomatic people with chiropractic adjustments. The determinants of this phenomenon need to be understood.", "gemini": "Bối cảnh Mặc dù có nhiều phương pháp thực hành trong nắn xương nhưng có thể xác định hai loại phụ, những người tập trung vào các vấn đề cơ xương và những người điều trị cả các vấn đề không liên quan đến cơ xương. Nhóm thứ hai có thể tuân theo mô hình 'trật khớp' bảo thủ cũ. Mục tiêu chính của nghiên cứu này là xác định liệu sinh viên nắn xương có quan điểm bảo thủ như vậy có khả năng có cách tiếp cận khác để xác định chống chỉ định, không chỉ định và chỉ định điều trị nắn xương so với những sinh viên không có quan điểm như vậy hay không. Phương pháp Một cuộc khảo sát ẩn danh và tự nguyện trên sinh viên nắn xương năm thứ 3 đến năm thứ 6 của Pháp đã được tiến hành từ tháng 11 năm 2017 đến tháng 1 năm 2018. Mức độ bảo thủ trong nắn xương (10 mục) và khả năng xác định chống chỉ định (2 trường hợp), không chỉ định (4 trường hợp) và chỉ định (3 trường hợp) đã được đánh giá thông qua bảng câu hỏi. Câu trả lời cho các trường hợp này được chia thành các câu trả lời 'phù hợp' và 'không phù hợp' theo định nghĩa của các nhóm nghiên cứu trước đây và nhóm nghiên cứu hiện tại. Mức độ bảo thủ được phân loại thành bốn nhóm, 'nhóm 4' tương ứng với điểm cao nhất. Dữ liệu mô tả được cung cấp và các phân tích lưỡng phân và đa biến được thực hiện thông qua hồi quy logistic để kiểm tra mối liên hệ giữa mức độ bảo thủ và khả năng xác định tính phù hợp của phương pháp điều trị nắn xương. Kết quả Trong số 536 sinh viên, có 359 sinh viên (67%) đã trả lời bảng câu hỏi. Nhìn chung, họ nhận ra một số chống chỉ định và chỉ định điều trị. Tuy nhiên, họ thấy khó xác định các trường hợp không chỉ định hơn. Những sinh viên bảo thủ hơn có nhiều khả năng có ý định điều trị bệnh nhân của họ, ngay cả khi điều này không liên quan (không chỉ định). Ví dụ, những người bảo thủ nhất (nhóm 4) sẵn sàng điều trị 'bằng nắn xương' cho một đứa trẻ 5 tuổi không có tiền sử đau lưng hoặc bệnh tật để ngăn ngừa đau lưng trong tương lai nhiều hơn so với những người trong nhóm 1 (OR = 14,7) và cũng để ngăn ngừa bệnh không liên quan đến cơ xương (OR = 22). Kết luận Điều đáng lo ngại là những sinh viên tuân theo mô hình trật khớp đã chuẩn bị 'vận hành' các quan điểm bảo thủ của họ trong phạm vi thực hành trong tương lai; rõ ràng là sẵn sàng điều trị những người không có triệu chứng bằng các biện pháp nắn xương. Cần hiểu được các yếu tố quyết định hiện tượng này."} +{"idx": 1900142, "en": "A new series of sidewall modified single-walled carbon nanotubes (SWCNTs) with perfluorophenyl molecules bearing carboxylic acid or methyl ester moieties are herein reported. Pristine and functionalized SWCNTs (p-SWCNTs and f-SWCNTs, respectively) were characterized by X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and scanning electron microscopy (SEM). The nitrene-based functionalization provided intact SWCNTs with methyl 4-azido-2,3,5,6-tetrafluorobenzoate (SWCNT-N-C6F4CO2CH3) and 4-azido-2,3,5,6-tetrafluorobenzoic acid (SWCNT-N-C6F4CO2H) attached every 213 and 109 carbon atoms, respectively. Notably, SWCNT-N-C6F4CO2H was sensitive in terms of the percentage of conductance variation from 5 to 40 ppm of ammonia (NH3) and trimethylamine (TMA) with a two-fold higher variation of conductance compared to p-SWCNTs at 40 ppm. The sensors are highly sensitive to NH3 and TMA as they showed very low responses (0.1%) toward 200 ppm of volatile organic compounds (VOCs) containing various functional groups representative of different classes of analytes such as benzene, tetrahydrofurane (THF), hexane, ethyl acetate (AcOEt), ethanol, acetonitrile (CH3CN), acetone and chloroform (CHCl3). Our system is a promising candidate for the realization of single-use chemiresistive sensors for the detection of threshold crossing by low concentrations of gaseous NH3 and TMA at room temperature.", "gemini": "Một loạt các ống nano cacbon thành đơn (SWCNT) có thành bên được sửa đổi với các phân tử perfluorophenyl mang các nhóm cacboxylic axit hoặc este metyl được báo cáo tại đây. Các SWCNT nguyên chất và được chức năng hóa (lần lượt là p-SWCNT và f-SWCNT) được đặc trưng bằng quang phổ điện tử tia X (XPS), quang phổ Raman và kính hiển vi điện tử quét (SEM). Chức năng hóa dựa trên nitren cung cấp các SWCNT nguyên vẹn với methyl 4-azido-2,3,5,6-tetrafluorobenzoat (SWCNT-N-C6F4CO2CH3) và axit 4-azido-2,3,5,6-tetrafluorobenzoic (SWCNT-N-C6F4CO2H) được gắn vào mỗi 213 và 109 nguyên tử cacbon, tương ứng. Đáng chú ý, SWCNT-N-C6F4CO2H rất nhạy về tỷ lệ phần trăm thay đổi độ dẫn từ 5 đến 40 ppm amoniac (NH3) và trimetylamin (TMA) với độ thay đổi độ dẫn gấp đôi so với p-SWCNT ở mức 40 ppm. Các cảm biến rất nhạy với NH3 và TMA vì chúng cho thấy phản ứng rất thấp (0,1%) đối với 200 ppm hợp chất hữu cơ dễ bay hơi (VOC) chứa nhiều nhóm chức năng đại diện cho các loại chất phân tích khác nhau như benzen, tetrahydrofuran (THF), hexan, etyl axetat (AcOEt), etanol, acetonitril (CH3CN), axeton và cloroform (CHCl3). Hệ thống của chúng tôi là một ứng cử viên triển vọng cho việc chế tạo các cảm biến điện trở hóa học dùng một lần để phát hiện ngưỡng vượt qua của nồng độ NH3 và TMA dạng khí thấp ở nhiệt độ phòng."} +{"idx": 1900143, "en": "Introduction The Child Health and Nutrition Research Initiative (CHNRI) method for health research prioritisation relies on stakeholders weighting criteria used to assess research options. These weights in turn impact on the final scores and ranks assigned to research options. Three quarters of CHNRI studies published to date have not involved stakeholders in criteria weighting. Of those that have, few incorporated members of the public into stakeholder groups. Those that have compared different stakeholder groups, such as donors, researchers, or policy makers, showed that different groups place different values upon CHNRI criteria. When choosing the composition of a stakeholder group, it may be important to understand factors that may influence weighting. Drawing upon a group of international public stakeholders, this study explores some of the effects of individual and demographic characteristics has on the weights assigned to the most commonly used CHNRI criteria, with the aim of informing future researchers on avoiding future biases. Methods Individual and demographic information and 5-point Likert scale responses to questions about the importance of 15 CHNRI criteria were collected from 1031 \"Turkers\" (Amazon Mechanical Turk workers) via Amazon Mechanical Turk (AMT), which is an online crowdsourcing platform. Thirteen of the fifteen criteria were analysed using random-intercept models and the remaining two were analysed through logistic regression. Results Self-reported health status explained most of the variability in participants' responses across criteria (11/15 criteria), followed by being female (10/15), ethnicity (9/15), employment (8/15), and religion (7/15). Differences across criteria indicate that when choosing stakeholder groups, researchers need to consider these factors to minimise bias. Conclusion Researchers should collect and report more detailed information from stakeholders, including individual and demographic characteristics, and ensure participation from both genders, multiple ethnicities, religious beliefs, and people with differing health statuses to be transparent regarding possible biases in health research prioritisation. Our analyses indicate that these factors do influence the relative importance of these values, even when the data appears fairly homogeneous.", "gemini": "Giới thiệu Phương pháp Sáng kiến Nghiên cứu Dinh dưỡng và Sức khỏe Trẻ em (CHNRI) để ưu tiên nghiên cứu sức khỏe dựa vào các bên liên quan cân nhắc các tiêu chí được sử dụng để đánh giá các lựa chọn nghiên cứu. Đến lượt mình, những trọng số này tác động đến số điểm cuối cùng và thứ hạng được gán cho các lựa chọn nghiên cứu. Ba phần tư các nghiên cứu của CHNRI được công bố cho đến nay không có sự tham gia của các bên liên quan trong việc cân nhắc tiêu chí. Trong số những nghiên cứu có sự tham gia của các bên liên quan, có rất ít nghiên cứu kết hợp các thành viên của công chúng vào các nhóm bên liên quan. Những nghiên cứu so sánh các nhóm bên liên quan khác nhau, chẳng hạn như các nhà tài trợ, nhà nghiên cứu hoặc nhà hoạch định chính sách, cho thấy các nhóm khác nhau đưa ra các giá trị khác nhau đối với các tiêu chí của CHNRI. Khi lựa chọn thành phần của một nhóm bên liên quan, điều quan trọng là phải hiểu các yếu tố có thể ảnh hưởng đến việc cân nhắc. Dựa trên một nhóm các bên liên quan công cộng quốc tế, nghiên cứu này khám phá một số tác động của các đặc điểm cá nhân và nhân khẩu học đối với các trọng số được gán cho các tiêu chí CHNRI được sử dụng phổ biến nhất, với mục đích cung cấp thông tin cho các nhà nghiên cứu trong tương lai về việc tránh các thành kiến trong tương lai. Phương pháp Thông tin cá nhân và nhân khẩu học cùng với các phản hồi thang Likert 5 điểm cho các câu hỏi về tầm quan trọng của 15 tiêu chí CHNRI đã được thu thập từ 1031 \"Turkers\" (công nhân của Amazon Mechanical Turk) thông qua Amazon Mechanical Turk (AMT), đây là một nền tảng cộng tác trực tuyến. Mười ba trong số mười lăm tiêu chí đã được phân tích bằng các mô hình chặn ngẫu nhiên và hai tiêu chí còn lại được phân tích thông qua hồi quy logistic. Kết quả Tình trạng sức khỏe tự báo cáo giải thích phần lớn sự thay đổi trong phản hồi của những người tham gia trên các tiêu chí (11/15 tiêu chí), tiếp theo là là nữ (10/15), dân tộc (9/15), nghề nghiệp (8/15) và tôn giáo (7/15). Sự khác biệt giữa các tiêu chí chỉ ra rằng khi lựa chọn các nhóm bên liên quan, các nhà nghiên cứu cần xem xét các yếu tố này để giảm thiểu sự thiên vị. Kết luận Các nhà nghiên cứu nên thu thập và báo cáo thông tin chi tiết hơn từ các bên liên quan, bao gồm các đặc điểm cá nhân và nhân khẩu học, đồng thời đảm bảo sự tham gia của cả hai giới, nhiều dân tộc, tín ngưỡng tôn giáo và những người có tình trạng sức khỏe khác nhau để minh bạch về các thành kiến có thể xảy ra trong việc ưu tiên nghiên cứu sức khỏe. Các phân tích của chúng tôi chỉ ra rằng các yếu tố này ảnh hưởng đến tầm quan trọng tương đối của các giá trị này, ngay cả khi dữ liệu có vẻ khá đồng nhất."} diff --git a/retranslated_vi_pubmed/chunk_190/error.json b/retranslated_vi_pubmed/chunk_190/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_190/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_191/completed.json b/retranslated_vi_pubmed/chunk_191/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..2f251ba5c24eaee4bfbdd1a649a6c427aa994316 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_191/completed.json @@ -0,0 +1,146 @@ +[ + 1910000, + 1910001, + 1910002, + 1910003, + 1910004, + 1910005, + 1910006, + 1910007, + 1910008, + 1910009, + 1910010, + 1910011, + 1910012, + 1910013, + 1910014, + 1910015, + 1910016, + 1910017, + 1910018, + 1910019, + 1910020, + 1910021, + 1910022, + 1910023, + 1910024, + 1910025, + 1910026, + 1910027, + 1910028, + 1910029, + 1910030, + 1910031, + 1910032, + 1910033, + 1910034, + 1910035, + 1910036, + 1910037, + 1910038, + 1910039, + 1910040, + 1910041, + 1910042, + 1910043, + 1910044, + 1910045, + 1910046, + 1910047, + 1910048, + 1910049, + 1910050, + 1910051, + 1910052, + 1910053, + 1910054, + 1910055, + 1910056, + 1910057, + 1910058, + 1910059, + 1910060, + 1910061, + 1910062, + 1910063, + 1910064, + 1910065, + 1910066, + 1910067, + 1910068, + 1910069, + 1910070, + 1910071, + 1910072, + 1910073, + 1910074, + 1910075, + 1910076, + 1910077, + 1910078, + 1910079, + 1910080, + 1910081, + 1910082, + 1910083, + 1910084, + 1910085, + 1910086, + 1910087, + 1910088, + 1910089, + 1910090, + 1910091, + 1910092, + 1910093, + 1910094, + 1910095, + 1910096, + 1910097, + 1910098, + 1910099, + 1910100, + 1910101, + 1910102, + 1910103, + 1910104, + 1910105, + 1910106, + 1910107, + 1910108, + 1910109, + 1910110, + 1910111, + 1910112, + 1910113, + 1910114, + 1910115, + 1910116, + 1910117, + 1910118, + 1910119, + 1910120, + 1910121, + 1910122, + 1910123, + 1910124, + 1910125, + 1910126, + 1910127, + 1910128, + 1910129, + 1910130, + 1910131, + 1910132, + 1910133, + 1910134, + 1910135, + 1910136, + 1910137, + 1910138, + 1910139, + 1910140, + 1910141, + 1910142, + 1910143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_191/data.jsonl b/retranslated_vi_pubmed/chunk_191/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..c1e73738f2ef983adddd0e46074bf2e1fa8bae8f --- /dev/null +++ b/retranslated_vi_pubmed/chunk_191/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1910000, "en": "The thalamocingulate tract is a key component of the Papez circuit that connects the anterior thalamic nucleus (ATN) to the cingulum bundle. While the other white matter connections, consisting of the fornix, cingulum bundle and mammillothalamic tract, were well defined in Papez's original 1937 paper, the anatomy of the thalamocingulate pathway was mentioned only in passing. Subsequent research has been unable to clarify the precise anatomical trajectory of this tract. In particular, the site of thalamocingulate tract interactions with the cingulum bundle have been inconsistently reported. This review aims to synthesize research on this least studied component of the Papez circuit. A systemic approach to reviewing historical anatomical dissection and neuronal tracing studies as well as contemporary diffusion magnetic resonance imaging studies of the thalamocingulate tract was undertaken across species. We found that although inconsistent, prior research broadly encompasses two differing descriptions of how the ATN interfaces with the cingulum after passing laterally through the anterior limb of the internal capsule. The first group of studies show that the pathway turns medially and rostrally and passes to the anterior cingulate region (Brodmann areas 24, 33, and 32) only. A second group suggests that the thalamocingulate tract interfaces with both the anterior and posterior cingulate (Brodmann areas 23 and 31) and retrosplenial region (Brodmann area 29). We discuss potential reasons for these discrepancies such as altering methodologies and species differences. We also discuss how these inconsistencies may be resolved in further research with refinements of terminology for the cingulate cortex and the thalamocingulate tract. Understanding the precise anatomical course of the last remaining unresolved final white matter tract in the Papez circuit may facilitate accurate investigation of the role of the complete Papez circuit in emotion and memory.", "gemini": "Đường thalamocingulate là một thành phần chính của mạch Papez kết nối nhân trước của đồi thị (ATN) với bó cingulum. Trong khi các kết nối chất trắng khác, bao gồm bó fornix, bó cingulum và bó mammillothalamic, được định nghĩa rõ ràng trong bài báo gốc năm 1937 của Papez, thì giải phẫu của đường thalamocingulate chỉ được đề cập thoáng qua. Các nghiên cứu tiếp theo vẫn chưa thể làm rõ được quỹ đạo giải phẫu chính xác của đường này. Đặc biệt, vị trí tương tác của đường thalamocingulate với bó cingulum đã được báo cáo không nhất quán. Mục đích của bài tổng quan này là tổng hợp các nghiên cứu về thành phần ít được nghiên cứu nhất của mạch Papez này. Một cách tiếp cận mang tính hệ thống để xem xét các nghiên cứu giải phẫu tử thi và nghiên cứu truy vết nơ-ron trong lịch sử cũng như các nghiên cứu hình ảnh cộng hưởng từ khuếch tán đương đại về đường thalamocingulate đã được thực hiện trên nhiều loài. Chúng tôi thấy rằng mặc dù không nhất quán, các nghiên cứu trước đây bao gồm hai mô tả khác nhau về cách ATN giao diện với cingulum sau khi đi ngang qua chi trước của bao trong. Nhóm nghiên cứu đầu tiên chỉ ra rằng đường đi theo hướng giữa và hướng mũi và chỉ đi đến vùng vành đai trước (vùng Brodmann 24, 33 và 32). Nhóm thứ hai cho thấy đường thalamocingulate giao diện với cả vành đai trước và sau (vùng Brodmann 23 và 31) và vùng retrosplenial (vùng Brodmann 29). Chúng tôi thảo luận về các lý do tiềm ẩn cho những sự khác biệt này như thay đổi phương pháp luận và sự khác biệt giữa các loài. Chúng tôi cũng thảo luận về cách giải quyết những điểm không nhất quán này trong các nghiên cứu tiếp theo với việc tinh chỉnh thuật ngữ cho vỏ vành đai và đường thalamocingulate. Việc hiểu được quá trình giải phẫu chính xác của đường chất trắng cuối cùng còn chưa được giải quyết trong mạch Papez có thể giúp điều tra chính xác vai trò của toàn bộ mạch Papez trong cảm xúc và trí nhớ."} +{"idx": 1910001, "en": "The Papez circuit has been considered as an important anatomical substrate involved in emotional experience. However, the circuit remains difficult to elucidate in the human brain due to the resolution limit of current neuroimaging modalities. In this article, for the first time, we report the direct visualization of the Papez circuit with 7-Tesla super-resolution magnetic resonance tractography. Two healthy, young male subjects (aged 30 and 35 years) were recruited as volunteers following the guidelines of the institutional review board (IRB). Track density imaging (TDI) generation with track tracing was performed using MRtrix software package. With these tools, we were able to visualize the entire Papez circuit. We believe this is the first study to visualize the complete loop of the Papez circuit, including the perforant path (PP), thalamocortical fibers of the anterior nucleus (AN), and mammillothalamic tract (MTT), which were hitherto difficult to visualize by conventional imaging techniques.", "gemini": "Mạch Papez được coi là một chất nền giải phẫu quan trọng liên quan đến trải nghiệm cảm xúc. Tuy nhiên, mạch này vẫn khó làm sáng tỏ trong não người do giới hạn độ phân giải của các phương thức chụp ảnh thần kinh hiện tại. Trong bài viết này, lần đầu tiên chúng tôi báo cáo hình ảnh trực tiếp của mạch Papez với phương pháp chụp ảnh cộng hưởng từ độ phân giải siêu cao 7 Tesla. Hai đối tượng nam trẻ tuổi khỏe mạnh (30 và 35 tuổi) được tuyển dụng làm tình nguyện viên theo hướng dẫn của hội đồng đánh giá thể chế (IRB). Tạo ảnh mật độ đường đi (TDI) với công cụ theo dõi đã được thực hiện bằng cách sử dụng gói phần mềm MRtrix. Với những công cụ này, chúng tôi có thể hình dung toàn bộ mạch Papez. Chúng tôi tin rằng đây là nghiên cứu đầu tiên hình dung được vòng lặp hoàn chỉnh của mạch Papez, bao gồm đường xuyên (PP), các sợi đồi thị của nhân trước (AN) và bó mammillothalamic (MTT), vốn trước đây khó có thể hình dung bằng các kỹ thuật chụp ảnh thông thường."} +{"idx": 1910002, "en": "Early-life experiences influence brain structure and function long-term, contributing to resilience or vulnerability to stress and stress-related disorders. Therefore, understanding the mechanisms by which early-life experiences program specific brain cells and circuits to shape life-long cognitive and emotional functions is crucial. We identify the population of corticotropin-releasing hormone (CRH)-expressing neurons in the hypothalamic paraventricular nucleus (PVN) as a key, early target of early-life experiences. Adverse experiences increase excitatory neurotransmission onto PVN CRH cells, whereas optimal experiences, such as augmented and predictable maternal care, reduce the number and function of glutamatergic inputs onto this cell population. Altered synaptic neurotransmission is sufficient to initiate large-scale, enduring epigenetic re-programming within CRH-expressing neurons, associated with stress resilience and additional cognitive and emotional outcomes. Thus, the mechanisms by which early-life experiences influence the brain provide tractable targets for intervention.", "gemini": "Những trải nghiệm thời thơ ấu ảnh hưởng đến cấu trúc và chức năng của não trong thời gian dài, góp phần vào khả năng phục hồi hoặc dễ bị căng thẳng và các rối loạn liên quan đến căng thẳng. Do đó, việc hiểu các cơ chế mà các trải nghiệm thời thơ ấu lập trình các tế bào và mạch não cụ thể để định hình các chức năng nhận thức và cảm xúc suốt đời là rất quan trọng. Chúng tôi xác định quần thể các tế bào thần kinh biểu hiện hormone giải phóng corticotropin (CRH) trong nhân quanh não thất vùng dưới đồi (PVN) là một mục tiêu chính, sớm của những trải nghiệm thời thơ ấu. Những trải nghiệm bất lợi làm tăng sự dẫn truyền thần kinh kích thích đến các tế bào CRH của PVN, trong khi những trải nghiệm tối ưu, chẳng hạn như sự chăm sóc của mẹ được tăng cường và có thể dự đoán được, làm giảm số lượng và chức năng của các đầu vào glutamatergic vào quần thể tế bào này. Sự dẫn truyền thần kinh synap bị thay đổi là đủ để khởi phát sự lập trình lại biểu sinh bền vững trên diện rộng trong các tế bào thần kinh biểu hiện CRH, liên quan đến khả năng phục hồi căng thẳng và các kết quả nhận thức và cảm xúc bổ sung. Do đó, các cơ chế mà những trải nghiệm thời thơ ấu ảnh hưởng đến não cung cấp các mục tiêu có thể điều trị được để can thiệp."} +{"idx": 1910003, "en": "While general self-referential processes and their neural underpinnings have been extensively investigated with neuroimaging tools, limited data is available on sex differences regarding self- and other-referential processing. To fill this gap, we measured 17 healthy women and men who performed a self- vs. other-appraisal task during functional magnetic resonance imaging (fMRI) using gender-stereotypical adjectives. During the self-appraisal task, typical male (e.g., \"dominant,\" \"competitive\") and female adjectives (e.g., \"communicative,\" \"sensitive\") were presented and participants were asked whether these adjectives applied to themselves. During the other-appraisal task, a prototypical male (Brad Pitt) and female actor (Julia Roberts) was presented and participants were asked again to judge whether typical male and female adjectives applied to these actors. Regarding self-referential processes, women ascribed significantly more female compared to male traits to themselves. At the same time both women and men indicated a stronger desire to exhibit male over female traits. While fMRI did not detect general sex differences in the self- and other-conditions, some subtle differences were revealed between the sexes: both in right putamen and bilateral amygdala stronger gender-congruent activation was found which was however not associated with behavioral measures like the number of self-ascribed female or male attributes. Furthermore, sex hormone levels showed some associations with brain activation pointing to a different pattern in women and men. Finally, the self- vs. other-condition in general led to stronger activation of the anterior cingulate cortex while the other- vs. self-condition activated the right precuneus more strongly which is in line with previous findings. To conclude, our data lend support for subtle sex differences during processing of stereotypical gender attributes. However, it remains unclear whether such differences have a behavioral relevance. We also point to several limitations of this study including the small sample size and the lack of control for potentially different hormonal states in women.", "gemini": "Mặc dù các quá trình tự tham chiếu chung và cơ sở thần kinh của chúng đã được nghiên cứu sâu rộng bằng các công cụ chụp ảnh thần kinh, dữ liệu giới hạn có sẵn về sự khác biệt giới tính liên quan đến quá trình tự tham chiếu và tham chiếu khác. Để lấp đầy khoảng trống này, chúng tôi đã đo 17 phụ nữ và nam giới khỏe mạnh thực hiện nhiệm vụ tự đánh giá so với đánh giá của người khác trong khi chụp cộng hưởng từ chức năng (fMRI) bằng cách sử dụng các tính từ rập khuôn giới tính. Trong nhiệm vụ tự đánh giá, các tính từ điển hình của nam giới (ví dụ: \"thống trị\", \"cạnh tranh\") và nữ giới (ví dụ: \"giao tiếp\", \"nhạy cảm\") đã được trình bày và những người tham gia được hỏi liệu những tính từ này có áp dụng cho bản thân họ hay không. Trong nhiệm vụ đánh giá của người khác, một nam diễn viên nguyên mẫu (Brad Pitt) và nữ diễn viên (Julia Roberts) đã được trình bày và những người tham gia một lần nữa được yêu cầu đánh giá xem các tính từ điển hình của nam giới và nữ giới có áp dụng cho những diễn viên này hay không. Về các quá trình tự tham chiếu, phụ nữ tự cho mình nhiều đặc điểm nữ tính hơn so với nam giới. Đồng thời, cả phụ nữ và nam giới đều cho biết mong muốn thể hiện các đặc điểm nam tính hơn nữ tính. Trong khi fMRI không phát hiện ra sự khác biệt chung về giới tính trong các điều kiện tự thân và điều kiện khác, một số khác biệt nhỏ đã được phát hiện giữa các giới: cả ở putamen bên phải và hạnh nhân hai bên đều phát hiện thấy hoạt động phù hợp với giới tính mạnh hơn, tuy nhiên không liên quan đến các biện pháp hành vi như số lượng các thuộc tính nữ hoặc nam được tự quy cho. Hơn nữa, nồng độ hormone giới tính cho thấy một số liên quan đến hoạt động của não chỉ ra một kiểu khác nhau ở phụ nữ và nam giới. Cuối cùng, điều kiện tự thân so với điều kiện khác nói chung dẫn đến sự kích hoạt mạnh hơn của vỏ não vành trước trong khi điều kiện khác so với điều kiện tự thân kích hoạt precuneus bên phải mạnh hơn, điều này phù hợp với các phát hiện trước đây. Để kết luận, dữ liệu của chúng tôi hỗ trợ cho sự khác biệt giới tính tinh tế trong quá trình xử lý các thuộc tính giới tính khuôn mẫu. Tuy nhiên, vẫn chưa rõ liệu những khác biệt như vậy có liên quan đến hành vi hay không. Chúng tôi cũng chỉ ra một số hạn chế của nghiên cứu này bao gồm quy mô mẫu nhỏ và thiếu kiểm soát đối với các trạng thái hormone có khả năng khác nhau ở phụ nữ."} +{"idx": 1910004, "en": "To what degree mental imagery (MI) bears on the same neuronal processes as perception has been a central question in the neurophysiological study of imagery. Sensory-recruitment models suggest that imagery of sensory material heavily relies on the involvement of sensory cortices. Empirical evidence mainly stems from the study of visual imagery and suggests that it depends on the mentally imagined material whether hierarchically lower regions are recruited. However, evidence from other modalities is necessary to infer generalized principles. In this fMRI study we used the somatotopic organization of the primary somatosensory cortex (SI) to test in how far MI of tactile sensations activates topographically sensory brain areas. Participants (N = 19) either perceived or imagined vibrotactile stimuli on their left or right thumbs or big toes. The direct comparison to a corresponding perception condition revealed that SI was somatotopically recruited during imagery. While stimulus driven bottom-up processing induced activity throughout all SI subareas, i.e., BA1, BA3a, BA3b, and BA2 defined by probabilistic cytoarchitectonic maps, top-down recruitment during imagery was limited to the hierarchically highest subarea BA2.", "gemini": "Mức độ mà hình ảnh tinh thần (MI) dựa trên cùng một quá trình thần kinh như nhận thức là một câu hỏi trung tâm trong nghiên cứu thần kinh sinh lý về hình ảnh. Các mô hình tuyển dụng cảm giác cho rằng hình ảnh của vật liệu cảm giác phụ thuộc nhiều vào sự tham gia của vỏ não cảm giác. Bằng chứng kinh nghiệm chủ yếu bắt nguồn từ nghiên cứu về hình ảnh thị giác và cho thấy nó phụ thuộc vào vật liệu được tưởng tượng trong tâm trí liệu các vùng thấp hơn trong hệ thống phân cấp có được tuyển dụng hay không. Tuy nhiên, bằng chứng từ các phương thức khác là cần thiết để suy ra các nguyên tắc tổng quát. Trong nghiên cứu fMRI này, chúng tôi đã sử dụng tổ chức somatotopy của vỏ somatosensory chính (SI) để kiểm tra xem MI của các cảm giác xúc giác kích hoạt các vùng não cảm giác theo địa hình ở mức độ nào. Những người tham gia (N = 19) hoặc cảm nhận hoặc tưởng tượng các kích thích rung trên ngón tay cái hoặc ngón chân cái bên trái hoặc bên phải của họ. So sánh trực tiếp với một điều kiện nhận thức tương ứng cho thấy SI được tuyển dụng theo địa hình trong quá trình hình ảnh. Trong khi quá trình xử lý theo hướng từ dưới lên do kích thích gây ra hoạt động trên tất cả các vùng phụ của SI, tức là BA1, BA3a, BA3b và BA2 được xác định bởi bản đồ kiến trúc tế bào xác suất, thì quá trình tuyển dụng theo hướng từ trên xuống trong quá trình hình ảnh bị giới hạn ở vùng phụ cao nhất trong hệ thống phân cấp BA2."} +{"idx": 1910005, "en": "This paper describes continuing research on the building of neurocognitive models of the internal mental and brain processes of children using a novel adapted combination of existing computational approaches and tools, and using electro-encephalographic (EEG) data to validate the models. The guiding working model which was pragmatically selected for investigation was the established and widely used Adaptive Control of Thought-Rational (ACT-R) modeling architecture from cognitive science. The anatomo-functional circuitry covered by ACT-R is validated by MRI-based neuroscience research. The present experimental data was obtained from a cognitive neuropsychology study involving preschool children (aged 4-6), which measured their visual selective attention and word comprehension behaviors. The collection and analysis of Event-Related Potentials (ERPs) from the EEG data allowed for the identification of sources of electrical activity known as dipoles within the cortex, using a combination of computational tools (Independent Component Analysis, FASTICA; EEG-Lab DIPFIT). The results were then used to build neurocognitive models based on Python ACT-R such that the patterns and the timings of the measured EEG could be reproduced as simplified symbolic representations of spikes, built through simplified electric-field simulations. The models simulated ultimately accounted for more than three-quarters of variations spatially and temporally in all electrical potential measurements (fit of model to dipole data expressed as R 2 ranged between 0.75 and 0.98; P < 0.0001). Implications for practical uses of the present work are discussed for learning and educational applications in non-clinical and special needs children's populations, and for the possible use of non-experts (teachers and parents).", "gemini": "Bài báo này mô tả nghiên cứu liên tục về việc xây dựng các mô hình nhận thức thần kinh về các quá trình tinh thần và não bộ bên trong của trẻ em bằng cách sử dụng sự kết hợp thích ứng mới lạ giữa các phương pháp và công cụ tính toán hiện có và sử dụng dữ liệu điện não đồ (EEG) để xác thực các mô hình. Mô hình làm việc hướng dẫn được lựa chọn thực dụng để điều tra là kiến trúc mô hình Kiểm soát thích ứng của tư duy lý trí (ACT-R) đã được thiết lập và sử dụng rộng rãi từ khoa học nhận thức. Mạch điện giải phẫu được ACT-R bao phủ được xác thực bởi nghiên cứu khoa học thần kinh dựa trên MRI. Dữ liệu thực nghiệm hiện tại thu được từ một nghiên cứu tâm lý thần kinh nhận thức liên quan đến trẻ mẫu giáo (4-6 tuổi), đo mức độ chú ý chọn lọc thị giác và hành vi hiểu từ của trẻ. Việc thu thập và phân tích các điện thế liên quan đến sự kiện (ERP) từ dữ liệu EEG cho phép xác định các nguồn hoạt động điện được gọi là lưỡng cực trong vỏ não, bằng cách sử dụng kết hợp các công cụ tính toán (Phân tích thành phần độc lập, FASTICA; EEG-Lab DIPFIT). Sau đó, các kết quả được sử dụng để xây dựng các mô hình nhận thức thần kinh dựa trên Python ACT-R sao cho các mẫu và thời gian của EEG được đo có thể được tái tạo thành các biểu diễn tượng trưng đơn giản của các xung, được xây dựng thông qua các mô phỏng trường điện đơn giản. Các mô hình được mô phỏng cuối cùng chiếm hơn ba phần tư các biến thể về mặt không gian và thời gian trong tất cả các phép đo điện thế (phù hợp của mô hình với dữ liệu lưỡng cực được biểu thị dưới dạng R 2 dao động trong khoảng 0,75 đến 0,98; P < 0,0001). Các ý nghĩa đối với việc sử dụng thực tế của công trình hiện tại được thảo luận cho các ứng dụng học tập và giáo dục ở trẻ em không phải lâm sàng và có nhu cầu đặc biệt, và cho việc sử dụng có thể có của những người không phải chuyên gia (giáo viên và phụ huynh)."} +{"idx": 1910006, "en": "This analysis follows our recent study showing that Canadian public reimbursement delays have lengthened from regulatory approval to listing decisions by public drug plans and delayed public access to innovative medicines, mainly due to processes following the Common Drug Review (CDR) and the pan-Canadian Oncology Drug Review (pCODR). Public drug plans participate in a pan-Canadian Pharmaceutical Alliance (pCPA) joint negotiation process before making decisions about whether or not to reimburse a product reviewed through CDR and pCODR. This research aims to report the findings from a comprehensive analysis of pCPA process times, times to reimbursement by public payers in Canada, and to explore the opportunities to reduce total delays in public reimbursement with a specific focus on the pCPA process. An analysis was conducted of pCPA timelines with respect to making decisions about products and indications reviewed through CDR/pCODR, and focusses on three separate time components: time to begin negotiating, time spent negotiating, and time to implement the negotiation (i.e., time to list) in each of nine jurisdictions (i.e., 10 provinces of Canada, excluding Quebec). This study demonstrates the role of post-CDR/pCODR processes in large and lengthening delays to listing new medicines. Notably, oncology products have experienced the longest increases in time to begin negotiating and to complete negotiations. Trends in listing times post-pCPA across provinces are less clear, however, it appears that consistency in terms of timelines across provinces is not happening quite so smoothly for oncology products compared to non-oncology products. Listing rates also appear to be declining for non-oncology products, although this trend is less conclusive for oncology products. Challenges need to be addressed to improve efficiency, transparency, and ultimately reduce pCPA timelines and total timelines to public reimbursement. Suggested ways to improve and streamline the listing process are: (1) transparent target timelines and associated performance incentives for the pCPA and public plan decisions, (2) parallel HTA-pCPA processes to enable pCPA negotiations to start part-way through the HTA review and allow pCPA negotiation information to be fed back into the HTA review, and (3) innovative agreements that consider patient input and earlier coverage with real-world evidence development.", "gemini": "Phân tích này theo sau nghiên cứu gần đây của chúng tôi cho thấy sự chậm trễ hoàn trả của công chúng Canada đã kéo dài từ phê duyệt theo quy định đến quyết định niêm yết của các chương trình thuốc công cộng và trì hoãn việc công chúng tiếp cận các loại thuốc sáng tạo, chủ yếu là do các quy trình theo Đánh giá thuốc chung (CDR) và Đánh giá thuốc ung thư toàn Canada (pCODR). Các chương trình thuốc công cộng tham gia vào quy trình đàm phán chung của Liên minh Dược phẩm toàn Canada (pCPA) trước khi đưa ra quyết định về việc có hoàn trả sản phẩm đã được xem xét thông qua CDR và pCODR hay không. Nghiên cứu này nhằm báo cáo các phát hiện từ phân tích toàn diện về thời gian xử lý của pCPA, thời gian hoàn trả của các bên thanh toán công cộng ở Canada và khám phá các cơ hội để giảm tổng thời gian chậm trễ hoàn trả công cộng với trọng tâm cụ thể vào quy trình pCPA. Một phân tích đã được tiến hành về các mốc thời gian của pCPA liên quan đến việc đưa ra quyết định về các sản phẩm và chỉ định được xem xét thông qua CDR/pCODR và tập trung vào ba thành phần thời gian riêng biệt: thời gian bắt đầu đàm phán, thời gian đàm phán và thời gian triển khai đàm phán (tức là thời gian niêm yết) tại mỗi chín khu vực pháp lý (tức là 10 tỉnh của Canada, không bao gồm Quebec). Nghiên cứu này chứng minh vai trò của các quy trình sau CDR/pCODR trong sự chậm trễ lớn và kéo dài đối với việc niêm yết các loại thuốc mới. Đáng chú ý, các sản phẩm ung thư đã trải qua thời gian tăng dài nhất để bắt đầu đàm phán và hoàn thành đàm phán. Tuy nhiên, xu hướng niêm yết thời gian sau pCPA trên các tỉnh không rõ ràng, tuy nhiên, có vẻ như tính nhất quán về thời gian trên các tỉnh không diễn ra suôn sẻ đối với các sản phẩm ung thư so với các sản phẩm không phải ung thư. Tỷ lệ niêm yết cũng có vẻ giảm đối với các sản phẩm không phải ung thư, mặc dù xu hướng này ít mang tính kết luận hơn đối với các sản phẩm ung thư. Cần giải quyết các thách thức để cải thiện hiệu quả, tính minh bạch và cuối cùng là rút ngắn thời gian của pCPA và thời gian hoàn trả công cộng. Các cách được đề xuất để cải thiện và hợp lý hóa quy trình niêm yết là: (1) mốc thời gian mục tiêu minh bạch và các ưu đãi về hiệu suất liên quan cho các quyết định của pCPA và kế hoạch công cộng, (2) các quy trình HTA-pCPA song song để cho phép các cuộc đàm phán của pCPA bắt đầu một phần thông qua đánh giá HTA và cho phép thông tin đàm phán của pCPA được đưa trở lại đánh giá HTA và (3) các thỏa thuận sáng tạo xem xét ý kiến của bệnh nhân và phạm vi bảo hiểm sớm hơn với sự phát triển bằng chứng thực tế."} +{"idx": 1910007, "en": "The management of cancer patients is frequently complicated by the occurrence of cachexia. This is a complex syndrome that markedly impacts on quality of life as well as on tolerance and response to anticancer treatments. Loss of body weight, wasting of both adipose tissue and skeletal muscle and reduced survival rates are among the main features of cachexia. Skeletal muscle wasting has been shown to depend, mainly at least, on the induction of protein degradation rates above physiological levels. Such hypercatabolic pattern is driven by overactivation of different intracellular proteolytic systems, among which those dependent on ubiquitin-proteasome and autophagy. Selective rather than bulk degradation of altered proteins and organelles was also proposed to occur. Within the picture described above, the muscle is frequently considered a sort of by-stander tissue where external stimuli, directly or indirectly, can poise protein metabolism toward a catabolic setting. By contrast, several observations suggest that the muscle reacts to the wasting drive imposed by cancer growth by activating different compensatory strategies that include anabolic capacity, the activation of autophagy and myogenesis. Even if muscle response is eventually ill-fated, its occurrence supports the idea that in the presence of appropriate treatments the development of cancer-induced wasting might not be an ineluctable event in tumor hosts.", "gemini": "Việc quản lý bệnh nhân ung thư thường trở nên phức tạp do tình trạng suy mòn xảy ra. Đây là một hội chứng phức tạp ảnh hưởng rõ rệt đến chất lượng cuộc sống cũng như khả năng dung nạp và đáp ứng với các phương pháp điều trị ung thư. Sụt cân, teo cả mô mỡ và cơ xương và giảm tỷ lệ sống sót là những đặc điểm chính của tình trạng suy mòn. Teo cơ xương đã được chứng minh là phụ thuộc chủ yếu vào việc tăng tốc độ phân hủy protein vượt quá mức sinh lý. Mô hình tăng dị hóa như vậy được thúc đẩy bởi sự hoạt động quá mức của các hệ thống phân giải protein trong tế bào khác nhau, trong đó có những hệ thống phụ thuộc vào ubiquitin-proteasome và autophagy. Người ta cũng đề xuất rằng sự phân hủy có chọn lọc thay vì phân hủy hàng loạt các protein và bào quan bị thay đổi. Trong bức tranh được mô tả ở trên, cơ thường được coi là một loại mô đứng ngoài, nơi các kích thích bên ngoài, trực tiếp hoặc gián tiếp, có thể định vị quá trình trao đổi chất protein theo hướng dị hóa. Ngược lại, một số quan sát cho thấy cơ phản ứng với động lực suy nhược do sự phát triển của ung thư bằng cách kích hoạt các chiến lược bù trừ khác nhau bao gồm khả năng đồng hóa, kích hoạt autophagy và sinh cơ. Ngay cả khi phản ứng của cơ cuối cùng là không may mắn, sự xuất hiện của nó vẫn hỗ trợ cho ý tưởng rằng khi có các phương pháp điều trị thích hợp, sự phát triển của tình trạng suy nhược do ung thư có thể không phải là một sự kiện không thể tránh khỏi ở những người mắc khối u."} +{"idx": 1910008, "en": "Neurovascular coupling refers to the mechanism that links the transient neural activity to the subsequent change in cerebral blood flow, which is regulated by both chemical signals and mechanical effects. Recent studies suggest that neurovascular coupling in neonates and preterm born infants is different compared to adults. The hemodynamic response after a stimulus is later and less pronounced and the stimulus might even result in a negative (hypoxic) signal. In addition, studies both in animals and neonates confirm the presence of a short hypoxic period after a stimulus in preterm infants. In clinical practice, different methodologies exist to study neurovascular coupling. The combination of functional magnetic resonance imaging or functional near-infrared spectroscopy (brain hemodynamics) with EEG (brain function) is most commonly used in neonates. Especially near-infrared spectroscopy is of interest, since it is a non-invasive method that can be integrated easily in clinical care and is able to provide results concerning longer periods of time. Therefore, near-infrared spectroscopy can be used to develop a continuous non-invasive measurement system, that could be used to study neonates in different clinical settings, or neonates with different pathologies. The main challenge for the development of a continuous marker for neurovascular coupling is how the coupling between the signals can be described. In practice, a wide range of signal interaction measures exist. Moreover, biomedical signals often operate on different time scales. In a more general setting, other variables also have to be taken into account, such as oxygen saturation, carbon dioxide and blood pressure in order to describe neurovascular coupling in a concise manner. Recently, new mathematical techniques were developed to give an answer to these questions. This review discusses these recent developments.", "gemini": "Liên hợp thần kinh mạch đề cập đến cơ chế liên kết hoạt động thần kinh thoáng qua với sự thay đổi tiếp theo của lưu lượng máu não, được điều chỉnh bởi cả tín hiệu hóa học và hiệu ứng cơ học. Các nghiên cứu gần đây cho thấy liên hợp thần kinh mạch ở trẻ sơ sinh và trẻ sinh non khác so với người lớn. Phản ứng huyết động sau kích thích diễn ra muộn hơn và ít rõ rệt hơn và kích thích thậm chí có thể dẫn đến tín hiệu âm (thiếu oxy). Ngoài ra, các nghiên cứu trên cả động vật và trẻ sơ sinh đều xác nhận sự hiện diện của một giai đoạn thiếu oxy ngắn sau khi kích thích ở trẻ sinh non. Trong thực hành lâm sàng, có các phương pháp khác nhau để nghiên cứu liên hợp thần kinh mạch. Sự kết hợp của chụp cộng hưởng từ chức năng hoặc quang phổ cận hồng ngoại chức năng (huyết động não) với EEG (chức năng não) được sử dụng phổ biến nhất ở trẻ sơ sinh. Đặc biệt quang phổ cận hồng ngoại rất được quan tâm, vì đây là phương pháp không xâm lấn có thể tích hợp dễ dàng trong chăm sóc lâm sàng và có thể cung cấp kết quả liên quan đến các khoảng thời gian dài hơn. Do đó, quang phổ cận hồng ngoại có thể được sử dụng để phát triển một hệ thống đo lường liên tục không xâm lấn, có thể được sử dụng để nghiên cứu trẻ sơ sinh trong các điều kiện lâm sàng khác nhau hoặc trẻ sơ sinh mắc các bệnh lý khác nhau. Thách thức chính đối với việc phát triển một dấu hiệu liên tục cho liên hợp thần kinh mạch là cách mô tả sự liên hợp giữa các tín hiệu. Trong thực tế, có rất nhiều biện pháp tương tác tín hiệu. Hơn nữa, các tín hiệu y sinh thường hoạt động trên các thang thời gian khác nhau. Trong một thiết lập chung hơn, các biến số khác cũng phải được tính đến, chẳng hạn như độ bão hòa oxy, carbon dioxide và huyết áp để mô tả liên hợp thần kinh mạch một cách súc tích. Gần đây, các kỹ thuật toán học mới đã được phát triển để đưa ra câu trả lời cho những câu hỏi này. Đánh giá này thảo luận về những phát triển gần đây này."} +{"idx": 1910009, "en": "hCLE/C14orf166/RTRAF, DDX1, and HSPC117 are components of cytoplasmic mRNA-transporting granules kinesin-associated in dendrites. They have also been found in cytoplasmic ribosome-containing RNA granules that transport specific mRNAs halted for translation until specific neuronal signals renders them accessible to the translation machinery. hCLE associates to DDX1, HSPC117, and FAM98B in HEK293T cells and all four proteins bind to cap analog-containing resins. Competition and elution experiments indicate that binding of hCLE complex to cap resins is independent of eIF4E; the cap-binding factor needed for translation. Purified hCLE free of its associated proteins binds cap with low affinity suggesting that its interacting proteins modulate its cap association. hCLE silencing reduces hCLE accumulation and that of its interacting proteins and decreases mRNA translation. hCLE-associated RNAs have been isolated and sequenced; RNAs involved in mRNA translation are specifically associated. The data suggest that RNA granules may co-transport RNAs encoding proteins involved in specific functions together with RNAs that encode proteins needed for the translation of these specific RNAs and indicate an important role for hCLE modulating mRNA translation.", "gemini": "hCLE/C14orf166/RTRAF, DDX1 và HSPC117 là các thành phần của các hạt vận chuyển mRNA ở tế bào chất liên kết với kinesin trong các sợi nhánh. Chúng cũng được tìm thấy trong các hạt RNA chứa ribosome ở tế bào chất vận chuyển các mRNA cụ thể bị dừng lại để dịch cho đến khi các tín hiệu thần kinh cụ thể làm cho chúng có thể tiếp cận được với bộ máy dịch. hCLE liên kết với DDX1, HSPC117 và FAM98B trong các tế bào HEK293T và cả bốn protein đều liên kết với các hạt nhựa chứa chất tương tự mũ. Các thí nghiệm cạnh tranh và giải elution chỉ ra rằng sự liên kết của phức hợp hCLE với các hạt nhựa chứa mũ không phụ thuộc vào eIF4E; yếu tố liên kết mũ cần thiết cho quá trình dịch. hCLE tinh khiết không có các protein liên quan liên kết với mũ có ái lực thấp, cho thấy rằng các protein tương tác của nó điều chỉnh sự liên kết mũ của nó. Sự im lặng của hCLE làm giảm sự tích tụ của hCLE và các protein tương tác của nó và làm giảm quá trình dịch mRNA. Các RNA liên kết với hCLE đã được phân lập và giải trình tự; các RNA liên quan đến quá trình dịch mRNA được liên kết đặc hiệu. Dữ liệu cho thấy rằng các hạt RNA có thể đồng vận chuyển các RNA mã hóa các protein tham gia vào các chức năng cụ thể cùng với các RNA mã hóa các protein cần thiết cho quá trình dịch các RNA cụ thể này và chỉ ra vai trò quan trọng của hCLE trong việc điều chỉnh quá trình dịch mRNA."} +{"idx": 1910010, "en": "It is not clear whether the association of increased peritoneal protein clearance (PPCl) with worse survival on peritoneal dialysis (PD) is a consequence of either local or systemic inflammation or indicative of generalized endothelial dysfunction associated with comorbidity. To investigate this we determined the relationship of PPCl to comorbidity, membrane area (equivalent to low molecular weight peritoneal solute transport rate), local and systemic inflammation and hypoalbuminaemia, and for each of these with patient survival. 257 incident patients from three GLOBAL Fluid Study centers were included in this analysis. Clinical profiles were collected at baseline along with a peritoneal equilibration test, 24-h dialysate protein and paired plasma and dialysate cytokine measurements. Although peritoneal protein clearance was associated with increased age and severe comorbidity on univariate analysis, only dialysate IL-6, peritoneal solute transport rate, plasma albumin and cardiac comorbidities (ischaemic heart disease and left ventricular dysfunction) were independent explanatory variables on multivariate analysis. While peritoneal protein clearance and daily peritoneal protein loss were associated with survival in univariate analysis, on multivariate analysis only plasma IL-6, age, residual kidney function, comorbidity, and plasma albumin were independent predictors. Peritoneal protein clearance is primarily a function of peritoneal membrane area and local membrane inflammation. The association with comorbidity and survival is predominantly explained by its inverse relationship to hypoalbuminaemia, especially in diabetics.", "gemini": "Không rõ liệu sự liên quan của việc tăng thanh thải protein phúc mạc (PPCl) với tình trạng sống sót tệ hơn khi chạy thận phúc mạc (PD) là hậu quả của tình trạng viêm tại chỗ hoặc toàn thân hay chỉ ra tình trạng rối loạn chức năng nội mô toàn thể liên quan đến bệnh đi kèm. Để nghiên cứu điều này, chúng tôi đã xác định mối quan hệ của PPCl với bệnh đi kèm, diện tích màng (tương đương với tốc độ vận chuyển chất tan phúc mạc trọng lượng phân tử thấp), tình trạng viêm tại chỗ và toàn thân và tình trạng giảm albumin máu và đối với mỗi yếu tố này với sự sống sót của bệnh nhân. 257 bệnh nhân mới từ ba trung tâm Nghiên cứu chất lỏng TOÀN CẦU đã được đưa vào phân tích này. Hồ sơ lâm sàng được thu thập lúc ban đầu cùng với xét nghiệm cân bằng phúc mạc, protein thẩm tách 24 giờ và các phép đo cytokine huyết tương và thẩm tách ghép đôi. Mặc dù thanh thải protein phúc mạc có liên quan đến tuổi tác tăng và bệnh đi kèm nghiêm trọng khi phân tích đơn biến, nhưng chỉ có IL-6 thẩm tách, tốc độ vận chuyển chất tan phúc mạc, albumin huyết tương và bệnh tim mạch đi kèm (bệnh tim thiếu máu cục bộ và rối loạn chức năng thất trái) là các biến giải thích độc lập khi phân tích đa biến. Trong khi thanh thải protein phúc mạc và mất protein phúc mạc hàng ngày có liên quan đến sự sống sót khi phân tích đơn biến, thì khi phân tích đa biến chỉ có IL-6 huyết tương, tuổi tác, chức năng thận còn lại, bệnh đi kèm và albumin huyết tương là các yếu tố dự báo độc lập. Thanh thải protein phúc mạc chủ yếu là chức năng của diện tích màng phúc mạc và tình trạng viêm màng tại chỗ. Sự liên quan với bệnh đi kèm và sự sống còn chủ yếu được giải thích bằng mối quan hệ nghịch đảo của nó với tình trạng giảm albumin máu, đặc biệt là ở bệnh nhân tiểu đường."} +{"idx": 1910011, "en": "Naked mole rats (Heterocephalus glaber) are among the most hypoxia-tolerant mammals, but their physiological responses to acute and chronic sustained hypoxia (CSH), and the molecular underpinnings of these responses, are poorly understood. In the present study we evaluated the acute hypoxic ventilatory response and the occurrence of ventilatory acclimatization to hypoxia following CSH exposure (8-10 days in 8% O2) of naked mole rats. We also investigated the role of excitatory glutamatergic signaling in the control of ventilation and metabolism in these conditions. Animals acclimated to normoxia (control) or CSH and then exposed to acute hypoxia (7% O2 for 1 h) exhibited elevated tidal volume (VT), but decreased breathing frequency (fR). As a result, total ventilation ( E) remained unchanged. Conversely, VT was lower in CSH animals relative to controls, suggesting that there is ventilatory plasticity following acclimatization to chronic hypoxia. Both control and CSH-acclimated naked mole rats exhibited similar 60-65% decreases in O2 consumption rate during acute hypoxia, and as a result their air convection requirement (ACR) increased ∼2.4 to 3-fold. Glutamatergic receptor inhibition decreased fR, E, and the rate of O2 consumption in normoxia but did not alter these ventilatory or metabolic responses to acute hypoxia in either the control or CSH groups. Taken together, these findings indicate that ventilatory acclimatization to hypoxia is atypical in naked mole rats, and glutamatergic signaling is not involved in their hypoxic ventilatory or metabolic responses to acute or chronic hypoxia.", "gemini": "Chuột chũi trụi lông (Heterocephalus glaber) là một trong những loài động vật có vú chịu được tình trạng thiếu oxy nhiều nhất, nhưng phản ứng sinh lý của chúng đối với tình trạng thiếu oxy cấp tính và mạn tính kéo dài (CSH) và cơ sở phân tử của những phản ứng này vẫn chưa được hiểu rõ. Trong nghiên cứu hiện tại, chúng tôi đã đánh giá phản ứng thông khí thiếu oxy cấp tính và sự xuất hiện của quá trình thích nghi thông khí với tình trạng thiếu oxy sau khi tiếp xúc với CSH (8-10 ngày trong môi trường 8% O2) của chuột chũi trụi lông. Chúng tôi cũng đã nghiên cứu vai trò của tín hiệu kích thích glutamat trong việc kiểm soát thông khí và chuyển hóa trong những điều kiện này. Các động vật thích nghi với tình trạng normoxia (kiểm soát) hoặc CSH và sau đó tiếp xúc với tình trạng thiếu oxy cấp tính (7% O2 trong 1 giờ) biểu hiện thể tích khí lưu thông (VT) tăng cao, nhưng tần số thở (fR) giảm. Do đó, tổng thông khí ( E) vẫn không thay đổi. Ngược lại, VT thấp hơn ở những động vật CSH so với nhóm đối chứng, điều này cho thấy có sự dẻo dai về thông khí sau khi thích nghi với tình trạng thiếu oxy mạn tính. Cả chuột chũi trụi lông thích nghi với tình trạng normoxia và CSH đều biểu hiện mức tiêu thụ O2 giảm tương tự 60-65% trong tình trạng thiếu oxy cấp tính và do đó, nhu cầu đối lưu không khí (ACR) của chúng tăng khoảng 2,4 đến 3 lần. Sự ức chế thụ thể glutamat làm giảm fR, E và tốc độ tiêu thụ O2 trong tình trạng normoxia nhưng không làm thay đổi những phản ứng thông khí hoặc chuyển hóa này đối với tình trạng thiếu oxy cấp tính ở cả nhóm đối chứng hoặc CSH. Kết hợp lại, những phát hiện này chỉ ra rằng sự thích nghi thông khí với tình trạng thiếu oxy là không điển hình ở chuột chũi trụi lông và tín hiệu glutamat không liên quan đến phản ứng thông khí hoặc chuyển hóa thiếu oxy cấp tính hoặc mạn tính của chúng."} +{"idx": 1910012, "en": "Tokyo 2020 Summer Olympics are projected to experience environmental heat stress that surpasses the environmental conditions observed in the Atlanta (1996), Athens (2004), Beijing (2008), and Rio (2016) Summer Olympics. This raises particular concerns for athletes who will likely to be exposed to extreme heat during the competitions. Therefore, in mass-participation event during warm season, it is vital for the hosting organization to build preparedness and resilience against heat, including appropriate treatment, and management strategies for exertional heat stroke (EHS). However, despite the existing literature regarding the evidence-based management of EHS, rectal thermometry and whole-body cold-water immersion are not readily accepted by medical professionals outside of the sports, and military medicine professionals. Current Japanese medical standard is no exception in falling behind on evidence-based management of EHS. Therefore, the first aim of this paper is to elucidate the inconsistency between the standard of care provided in Japan for EHS and what has been accepted as the gold standard by the scientific literature. The second aim of this paper is to provide optimal EHS management strategies that should be implemented at the Tokyo 2020 Summer Olympics from organizational level to maximize the safety of athletes and to improve organizational resilience to heat. The risk of extreme heat is often neglected until a catastrophic incidence occurs. It is vital for the Japanese medical leadership and athletic communities to re-examine the current EHS management strategies and implement evidence-based countermeasure for EHS to expand the application of scientific knowledge.", "gemini": "Dự kiến Thế vận hội mùa hè Tokyo 2020 sẽ phải trải qua tình trạng căng thẳng do nhiệt độ môi trường vượt quá các điều kiện môi trường được ghi nhận trong Thế vận hội mùa hè Atlanta (1996), Athens (2004), Bắc Kinh (2008) và Rio (2016). Điều này làm dấy lên những lo ngại đặc biệt đối với các vận động viên, những người có khả năng phải tiếp xúc với nhiệt độ khắc nghiệt trong suốt các cuộc thi. Do đó, trong các sự kiện có sự tham gia của đông đảo quần chúng vào mùa nóng, điều quan trọng đối với tổ chức chủ nhà là phải xây dựng sự chuẩn bị và khả năng phục hồi trước sức nóng, bao gồm cả việc điều trị thích hợp và các chiến lược quản lý đối với tình trạng say nắng do gắng sức (EHS). Tuy nhiên, mặc dù có các tài liệu hiện có liên quan đến việc quản lý EHS dựa trên bằng chứng, nhưng việc đo nhiệt độ trực tràng và ngâm toàn thân trong nước lạnh vẫn chưa được các chuyên gia y tế bên ngoài lĩnh vực y học thể thao và quân y chấp nhận rộng rãi. Tiêu chuẩn y tế hiện tại của Nhật Bản cũng không phải là ngoại lệ khi tụt hậu trong việc quản lý EHS dựa trên bằng chứng. Do đó, mục đích đầu tiên của bài báo này là làm sáng tỏ sự không nhất quán giữa tiêu chuẩn chăm sóc được cung cấp tại Nhật Bản đối với EHS và tiêu chuẩn được chấp nhận là tiêu chuẩn vàng bởi các tài liệu khoa học. Mục đích thứ hai của bài báo này là cung cấp các chiến lược quản lý EHS tối ưu cần được triển khai tại Thế vận hội mùa hè Tokyo 2020 từ cấp độ tổ chức để tối đa hóa sự an toàn cho các vận động viên và cải thiện khả năng phục hồi của tổ chức trước sức nóng. Rủi ro do nhiệt độ khắc nghiệt thường bị bỏ qua cho đến khi xảy ra một sự cố thảm khốc. Điều quan trọng đối với các nhà lãnh đạo y tế và cộng đồng thể thao Nhật Bản là phải xem xét lại các chiến lược quản lý EHS hiện tại và triển khai các biện pháp đối phó dựa trên bằng chứng đối với EHS để mở rộng phạm vi ứng dụng của kiến thức khoa học."} +{"idx": 1910013, "en": "Ischemic heart disease including myocardial infarction (MI) is a major cause of mortality and morbidity worldwide. In order to manage the acute myocardial infarction (AMI) outbreaks, novel biomarkers for risk prediction are needed. Recent studies have shown that circulating microRNAs (miRNAs) are promising biomarkers for cardiovascular diseases prediction. This study aimed to determine the possibility of circulating miRNAs used as biomarkers for AMI. The dynamic expression levels of miRNAs were examined before and after percutaneous coronary intervention (PCI) in patients. Circulating miR-17-5p, miR-126-5p, and miR-145-3p were selected and validated in 29 patients with AMI and 21 matched controls by quantitative real-time PCR. The expression levels of plasma miR-17-5p, miR-126-5p, and miR-145-3p were significantly increased in AMI patients. Receiver Operating Characteristic (ROC) analysis indicated that miR-17-5p, miR-126-5p, and miR-145-3p showed considerable diagnostic efficiency for AMI. Furthermore, we demonstrated that the combination of these three miRNAs managed to provide more accurate diagnosing of AMI.", "gemini": "Bệnh tim thiếu máu cục bộ bao gồm nhồi máu cơ tim (MI) là nguyên nhân chính gây tử vong và bệnh tật trên toàn thế giới. Để quản lý các đợt bùng phát nhồi máu cơ tim cấp (AMI), cần có các dấu ấn sinh học mới để dự đoán rủi ro. Các nghiên cứu gần đây đã chỉ ra rằng microRNA (miRNA) lưu hành là những dấu ấn sinh học đầy hứa hẹn để dự đoán các bệnh tim mạch. Nghiên cứu này nhằm xác định khả năng sử dụng miRNA lưu hành làm dấu ấn sinh học cho AMI. Nồng độ biểu hiện động của miRNA đã được kiểm tra trước và sau can thiệp mạch vành qua da (PCI) ở bệnh nhân. miRNA-17-5p, miRNA-126-5p và miRNA-145-3p lưu hành đã được lựa chọn và xác nhận ở 29 bệnh nhân AMI và 21 đối chứng phù hợp bằng phản ứng chuỗi polymerase thời gian thực định lượng. Nồng độ biểu hiện của miRNA-17-5p, miRNA-126-5p và miRNA-145-3p trong huyết tương tăng đáng kể ở bệnh nhân AMI. Phân tích đặc điểm hoạt động của người nhận (ROC) chỉ ra rằng miRNA-17-5p, miRNA-126-5p và miRNA-145-3p cho thấy hiệu quả chẩn đoán đáng kể đối với AMI. Hơn nữa, chúng tôi đã chứng minh rằng sự kết hợp của ba miRNA này có thể cung cấp khả năng chẩn đoán AMI chính xác hơn."} +{"idx": 1910014, "en": "Background: Families with parental mental health issues often have numerous problems needing multilevel measures to address them. The \"Let's Talk about Children Service Model (LT-SM)\" is a community-based service approach aiming at collectively impacting population needs regarding child protection services. Three municipalities in the Raahe District (RD) of Finland requested implementation of the LT Service Model. This paper describes the model and first results. Methods: The LT Service Model connects relevant stakeholders with families and their social networks aiming at the shared goal of supporting children's everyday life at home, kindergarten, school, and leisure environments. Parents, teachers, and other caretakers are supported by LT interventions. An infrastructure for collaboration, decision making, monitoring, training, and feedback is established, embracing health, social and educational services, and other stakeholders. Referrals to child protection services were compared with national data before (2009-2013) and after implementation of the LT Service Model (2013-2016). Analyses were conducted using the joinpoint regression method. Results: There was a significant decrease in the underage population referred to child protection services in RD (AAPC = -6.9; p = 0.013) between 2013 and 2016, in contrast with an increased rate nationwide (AAPC = 1.9; p = 0.020). Conclusion: In the LT Service Model, prevention starts in children's everyday life as the uniting, common goal for multiple stakeholders and an integrated service structure is developed to support this effort. The first results are promising, showing an appreciable decrease in referrals to child protection services, although further research with longer follow-up and across other municipalities is needed.", "gemini": "Bối cảnh: Các gia đình có vấn đề sức khỏe tâm thần của cha mẹ thường gặp nhiều vấn đề cần có các biện pháp đa cấp để giải quyết. \"Mô hình dịch vụ Hãy nói về trẻ em (LT-SM)\" là một cách tiếp cận dịch vụ dựa vào cộng đồng nhằm mục đích tác động chung đến nhu cầu của dân số liên quan đến các dịch vụ bảo vệ trẻ em. Ba thành phố ở Quận Raahe (RD) của Phần Lan đã yêu cầu triển khai Mô hình dịch vụ LT. Bài báo này mô tả mô hình và những kết quả đầu tiên. Phương pháp: Mô hình dịch vụ LT kết nối các bên liên quan có liên quan với các gia đình và mạng lưới xã hội của họ nhằm mục tiêu chung là hỗ trợ cuộc sống hàng ngày của trẻ em tại nhà, trường mẫu giáo, trường học và môi trường giải trí. Cha mẹ, giáo viên và những người chăm sóc khác được hỗ trợ bởi các can thiệp của LT. Một cơ sở hạ tầng cho sự hợp tác, ra quyết định, giám sát, đào tạo và phản hồi được thiết lập, bao gồm các dịch vụ y tế, xã hội và giáo dục cùng các bên liên quan khác. Các trường hợp chuyển đến các dịch vụ bảo vệ trẻ em đã được so sánh với dữ liệu quốc gia trước (2009-2013) và sau khi triển khai Mô hình dịch vụ LT (2013-2016). Các phân tích được thực hiện bằng phương pháp hồi quy điểm nối. Kết quả: Có sự sụt giảm đáng kể trong dân số chưa đủ tuổi được chuyển đến các dịch vụ bảo vệ trẻ em tại RD (AAPC = -6,9; p = 0,013) trong giai đoạn 2013-2016, trái ngược với tỷ lệ gia tăng trên toàn quốc (AAPC = 1,9; p = 0,020). Kết luận: Trong Mô hình dịch vụ LT, công tác phòng ngừa bắt đầu từ cuộc sống hàng ngày của trẻ em với tư cách là mục tiêu chung, thống nhất đối với nhiều bên liên quan và một cấu trúc dịch vụ tích hợp được phát triển để hỗ trợ nỗ lực này. Những kết quả đầu tiên rất hứa hẹn, cho thấy sự sụt giảm đáng kể trong các trường hợp chuyển đến các dịch vụ bảo vệ trẻ em, mặc dù cần có thêm nghiên cứu với thời gian theo dõi dài hơn và trên các thành phố khác."} +{"idx": 1910015, "en": "Despite many studies documenting the prevalence of various co-occurring psychiatric symptoms in children and adults with ASD, less is known about how these symptoms relate to subtypes defined by particular phenotypic features within the ASD population. We examined the severity and prevalence of comorbid symptoms of psychopathology, emotion dysregulation, and maladaptive behaviors, as well as adaptive functioning, in a group of 65 minimally verbal children (n = 33) and adolescents (n = 32) with ASD. On the Child and Adolescent Symptom Inventory (CASI-5), for all the symptom classifications except oppositional defiant disorder and conduct disorder, more participants in our sample showed elevated or clinically concerning severity scores relative to the general population. On the Emotion Dysregulation Inventory (EDI), the mean scores for Reactivity and Dysphoria factors in our sample were lower than in the autism calibration sample, which included a large number of inpatient youth with ASD. Overall, few differences were found between the children and adolescents within this severely impaired group of ASD individuals based on clinical cutoff scores on the CASI-5 and EDI factor scores. Psychiatric comorbidities and emotion dysregulation measures were not correlated with autism symptom severity or with measures of adaptive functioning, and were largely unrelated to IQ in our sample. The number of clinically significant psychiatric symptoms on the CASI-5 emerged as the main predictor of maladaptive behaviors. Findings suggest a wide range of co-occurring psychopathology and high degree of maladaptive behavior among minimally verbal children and adolescents with ASD, which are not directly attributable to autism symptom severity, intellectual disability or limitations in adaptive functioning.", "gemini": "Mặc dù có nhiều nghiên cứu ghi nhận sự phổ biến của nhiều triệu chứng tâm thần đồng thời xuất hiện ở trẻ em và người lớn mắc ASD, nhưng người ta lại biết ít hơn về mối liên hệ giữa các triệu chứng này với các phân nhóm được xác định bởi các đặc điểm biểu hiện cụ thể trong quần thể ASD. Chúng tôi đã kiểm tra mức độ nghiêm trọng và tỷ lệ mắc các triệu chứng bệnh lý tâm thần đồng mắc, rối loạn cảm xúc và hành vi thích nghi kém, cũng như chức năng thích nghi, trong một nhóm gồm 65 trẻ em ít nói (n = 33) và thanh thiếu niên (n = 32) mắc ASD. Trên Bản kiểm kê triệu chứng ở trẻ em và thanh thiếu niên (CASI-5), đối với tất cả các phân loại triệu chứng ngoại trừ rối loạn chống đối và rối loạn hành vi, nhiều người tham gia trong mẫu của chúng tôi cho thấy điểm số nghiêm trọng tăng cao hoặc đáng lo ngại về mặt lâm sàng so với dân số chung. Trên Bản kiểm kê rối loạn cảm xúc (EDI), điểm số trung bình cho các yếu tố Phản ứng và Rối loạn cảm xúc trong mẫu của chúng tôi thấp hơn so với mẫu hiệu chuẩn tự kỷ, bao gồm một số lượng lớn thanh thiếu niên nội trú mắc ASD. Nhìn chung, có rất ít sự khác biệt giữa trẻ em và thanh thiếu niên trong nhóm cá nhân mắc ASD bị suy giảm nghiêm trọng này dựa trên điểm cắt lâm sàng trên CASI-5 và điểm số yếu tố EDI. Các bệnh tâm thần đồng mắc và các biện pháp điều chỉnh cảm xúc không tương quan với mức độ nghiêm trọng của triệu chứng tự kỷ hoặc với các biện pháp chức năng thích nghi và phần lớn không liên quan đến IQ trong mẫu của chúng tôi. Số lượng các triệu chứng tâm thần có ý nghĩa lâm sàng trên CASI-5 nổi lên như yếu tố dự báo chính của hành vi thích nghi kém. Các phát hiện cho thấy một loạt các bệnh lý tâm thần đồng mắc và mức độ hành vi thích nghi kém cao ở trẻ em và thanh thiếu niên ít nói mắc ASD, không trực tiếp liên quan đến mức độ nghiêm trọng của triệu chứng tự kỷ, khuyết tật trí tuệ hoặc hạn chế trong chức năng thích nghi."} +{"idx": 1910016, "en": "The aim of this meta-analysis was to review studies that applied musical instrument playing as an intervention to improve cognitive functioning of older adults with and without cognitive impairment. English-language articles published between 1990 and 2018 were searched using electronic databases. Music therapy journals were also hand searched for relevant research. Inclusion criteria for participants were older adults, ages 60 years and older, and any clinical diagnosis of cognitive impairment had to be due to aging. Searches used combinations of the following keywords: older adults, instrument playing, and cognitive outcomes measures. A total of 10 studies that met the inclusion criteria were included in the final analysis: five studies with healthy older adults, two with older adults with mild cognitive impairment (MCI), two studies with older adults with dementia, and one study with both healthy older adults and older adults with MCI. The results of this meta-analysis demonstrated that different types of cognitive involvement were demanded from instrument playing. Furthermore, depending on the type of involvement, a target cognitive domain was found to be differentially impacted by the instrument playing intervention. This study supports using different types of instrument playing for interventions targeting specific cognitive domains of older adults with varying levels of cognitive aging.", "gemini": "Mục đích của phân tích tổng hợp này là xem xét các nghiên cứu áp dụng việc chơi nhạc cụ như một biện pháp can thiệp để cải thiện chức năng nhận thức của người lớn tuổi có và không bị suy giảm nhận thức. Các bài báo tiếng Anh được xuất bản trong giai đoạn 1990-2018 được tìm kiếm bằng các cơ sở dữ liệu điện tử. Các tạp chí về liệu pháp âm nhạc cũng được tìm kiếm thủ công để tìm các nghiên cứu có liên quan. Tiêu chuẩn tuyển chọn đối với những người tham gia là người lớn tuổi, từ 60 tuổi trở lên và bất kỳ chẩn đoán lâm sàng nào về suy giảm nhận thức đều phải do lão hóa. Các tìm kiếm sử dụng kết hợp các từ khóa sau: người lớn tuổi, chơi nhạc cụ và các biện pháp kết quả nhận thức. Tổng cộng có 10 nghiên cứu đáp ứng các tiêu chuẩn tuyển chọn đã được đưa vào phân tích cuối cùng: năm nghiên cứu với người lớn tuổi khỏe mạnh, hai nghiên cứu với người lớn tuổi bị suy giảm nhận thức nhẹ (MCI), hai nghiên cứu với người lớn tuổi bị mất trí nhớ và một nghiên cứu với cả người lớn tuổi khỏe mạnh và người lớn tuổi bị MCI. Kết quả của phân tích tổng hợp này chứng minh rằng chơi nhạc cụ đòi hỏi các loại tham gia nhận thức khác nhau. Hơn nữa, tùy thuộc vào loại tham gia, một phạm vi nhận thức mục tiêu được phát hiện bị ảnh hưởng khác nhau bởi sự can thiệp chơi nhạc cụ. Nghiên cứu này hỗ trợ việc sử dụng các loại chơi nhạc cụ khác nhau để can thiệp nhắm vào các phạm vi nhận thức cụ thể của người lớn tuổi có các mức độ lão hóa nhận thức khác nhau."} +{"idx": 1910017, "en": "The Doctrine of the Mean (zhongyong) introduced by Confucianism is not only an aspect of faith, but also a way of thinking for Chinese individuals. Zhongyong includes two thinking forms: eclectic thinking (ET; i.e., \"neither-A-nor-B\") and integrated thinking (IT; i.e., \"both-A-and-B\"). Given the inclination of Asian individuals toward situational cognition, this study used questions about situations familiar to Chinese undergraduates to activate either ET or IT. This was done to investigate the effects of the two divergent thinking forms of zhongyong on performance levels on the Remote Associates Test (RAT). Both behavioral and EEG results found that participants in the IT condition demonstrated higher RAT scores than those in the ET condition. The conclusion was that the RAT and priming tasks shared the same neural mechanism. This meant that the priming tasks of IT allowed participants to enter a state of creative preparation in advance, further affecting resolution of the RAT.", "gemini": "Học thuyết Trung dung (trung dung) do Nho giáo đưa ra không chỉ là một khía cạnh của đức tin, mà còn là một cách suy nghĩ của người Trung Quốc. Trung dung bao gồm hai hình thức tư duy: tư duy chiết trung (ET; tức là \"không-A-cũng-không-B\") và tư duy tích hợp (IT; tức là \"vừa-A-vừa-B\"). Do khuynh hướng của người châu Á hướng đến nhận thức tình huống, nghiên cứu này đã sử dụng các câu hỏi về các tình huống quen thuộc đối với sinh viên đại học Trung Quốc để kích hoạt ET hoặc IT. Điều này được thực hiện để điều tra các tác động của hai hình thức tư duy khác nhau của trung dung đối với mức độ thực hiện trên Bài kiểm tra liên tưởng từ xa (RAT). Cả kết quả hành vi và EEG đều phát hiện ra rằng những người tham gia trong điều kiện IT đạt điểm RAT cao hơn so với những người trong điều kiện ET. Kết luận là RAT và các nhiệm vụ chuẩn bị có chung cơ chế thần kinh. Điều này có nghĩa là các nhiệm vụ chuẩn bị của IT cho phép người tham gia vào trạng thái chuẩn bị sáng tạo trước, ảnh hưởng thêm đến việc giải quyết RAT."} +{"idx": 1910018, "en": "Background: Previous research has shown that quality of life for adults decreases when they become parents, remains at a lower level than of non-parents and declines further with each child they have. Consistent with this, parents report that having children leads to more daily struggles and concerns than their work outside the home. In this study, we have investigated how participating in a brief parent training intervention influences parents' quality of life. The aim of the study was to evaluate whether a brief, six-session version of an evidence-based parent training program (The Incredible Years), delivered in a non-clinical community sample, changed parent quality of life up to four years after the initial intervention. Methods: Data were collected from mothers and fathers in a randomized controlled community trial (N = 117). Children's mean age was 3.95 years at baseline, and 7.5 years at 4-year follow-up. Results: There were no significant differences in the trajectory of change over the four time points between the intervention and control groups for mothers or fathers. However, results from analysing the linear change from pre to each of the other measurement points, revealed statistically significantly different change on life satisfaction after completing the intervention compared to the control group; immediately following the intervention, t(357) = 2.76, p = 0.006; and the difference between the groups was maintained three years after the intervention, t(360) = 3.14, p = 0.002. Conclusion: The results of this study suggest that offering a parenting program focused on building a positive parent-child relation, has the potential to improve mothers' quality of life. Implications of this are discussed. Clinical Trial Registration: www.ClinicalTrials.gov, identifier NCT02850510. Retrospectively registered 29 July 2016.", "gemini": "Lý lịch: Các nghiên cứu trước đây đã chỉ ra rằng chất lượng cuộc sống của người lớn giảm khi họ trở thành cha mẹ, duy trì ở mức thấp hơn so với những người không phải là cha mẹ và giảm thêm với mỗi đứa con. Phù hợp với điều này, các bậc cha mẹ báo cáo rằng việc có con dẫn đến nhiều khó khăn và lo lắng hàng ngày hơn là công việc của họ bên ngoài gia đình. Trong nghiên cứu này, chúng tôi đã điều tra cách tham gia vào một can thiệp đào tạo phụ huynh ngắn hạn ảnh hưởng đến chất lượng cuộc sống của cha mẹ. Mục đích của nghiên cứu là đánh giá xem phiên bản sáu buổi ngắn gọn của chương trình đào tạo phụ huynh dựa trên bằng chứng (The Incredible Years), được cung cấp trong một mẫu cộng đồng không lâm sàng, có thay đổi chất lượng cuộc sống của cha mẹ hay không cho đến bốn năm sau can thiệp ban đầu. Phương pháp: Dữ liệu được thu thập từ các bà mẹ và các ông bố trong một thử nghiệm cộng đồng có đối chứng ngẫu nhiên (N = 117). Độ tuổi trung bình của trẻ là 3,95 tuổi khi bắt đầu và 7,5 tuổi sau 4 năm theo dõi. Kết quả: Không có sự khác biệt đáng kể nào trong quỹ đạo thay đổi trong bốn thời điểm giữa nhóm can thiệp và nhóm đối chứng đối với các bà mẹ hoặc các ông bố. Tuy nhiên, kết quả phân tích sự thay đổi tuyến tính từ trước đến từng điểm đo khác cho thấy sự thay đổi có ý nghĩa thống kê đáng kể về sự hài lòng trong cuộc sống sau khi hoàn thành can thiệp so với nhóm đối chứng; ngay sau can thiệp, t(357) = 2,76, p = 0,006; và sự khác biệt giữa các nhóm vẫn được duy trì ba năm sau can thiệp, t(360) = 3,14, p = 0,002. Kết luận: Kết quả của nghiên cứu này cho thấy rằng việc cung cấp một chương trình nuôi dạy con cái tập trung vào việc xây dựng mối quan hệ cha mẹ - con cái tích cực có khả năng cải thiện chất lượng cuộc sống của các bà mẹ. Ý nghĩa của điều này được thảo luận. Đăng ký thử nghiệm lâm sàng: www.ClinicalTrials.gov, mã định danh NCT02850510. Đăng ký hồi cứu ngày 29 tháng 7 năm 2016."} +{"idx": 1910019, "en": "Parenting behavior and practices contribute to the intergenerational relationship between parent and child anxiety, with parental control being a consistent predictor of child anxiety. Parental experiential avoidance refers to how a parent copes with their internal world in the context of parenting. Little is known about how this relatively new parenting concept relates to child anxiety. The current study tested the indirect effect of parent anxiety on child anxiety through parental control and parental experiential avoidance; the indirect effect of parent anxiety on parental control through parental experiential avoidance; and the moderating effect of parental experiential avoidance on the relationship between parental control and child anxiety. Using a cross-sectional design, parents (N = 85) from a community sample of 8-12-year-old children self-reported on a survey measuring parent anxiety, child anxiety, parental control, and parental experiential avoidance. A hierarchical regression indicated that parental experiential avoidance significantly predicted child anxiety and accounted for further variance in child anxiety, over, and above parental control. There was an indirect effect of parent anxiety on child anxiety through parental control and parental experiential avoidance. Parental experiential avoidance moderated the relationship between parental control and child anxiety, such that the relationship was only significant at high levels of parental experiential avoidance. The current study provides support for the role of parental experiential avoidance in an intergenerational understanding of anxiety. Future research should replicate the study with a clinical sample. Theoretical and practice implications are considered.", "gemini": "Hành vi và thực hành nuôi dạy con cái góp phần vào mối quan hệ giữa các thế hệ giữa cha mẹ và sự lo lắng của trẻ, trong đó sự kiểm soát của cha mẹ là một yếu tố dự báo nhất quán về sự lo lắng của trẻ. Tránh né trải nghiệm của cha mẹ đề cập đến cách một người cha mẹ đối phó với thế giới nội tâm của họ trong bối cảnh làm cha mẹ. Người ta biết rất ít về cách mà khái niệm nuôi dạy con cái tương đối mới này liên quan đến sự lo lắng của trẻ. Nghiên cứu hiện tại đã kiểm tra tác động gián tiếp của sự lo lắng của cha mẹ đối với sự lo lắng của trẻ thông qua sự kiểm soát của cha mẹ và sự tránh né trải nghiệm của cha mẹ; tác động gián tiếp của sự lo lắng của cha mẹ đối với sự kiểm soát của cha mẹ thông qua sự tránh né trải nghiệm của cha mẹ; và tác động điều chỉnh của sự tránh né trải nghiệm của cha mẹ đối với mối quan hệ giữa sự kiểm soát của cha mẹ và sự lo lắng của trẻ. Sử dụng thiết kế cắt ngang, cha mẹ (N = 85) từ một mẫu cộng đồng gồm trẻ em từ 8-12 tuổi tự báo cáo trên một cuộc khảo sát đo lường sự lo lắng của cha mẹ, sự lo lắng của trẻ, sự kiểm soát của cha mẹ và sự tránh né trải nghiệm của cha mẹ. Một hồi quy phân cấp chỉ ra rằng sự tránh né trải nghiệm của cha mẹ dự đoán đáng kể sự lo lắng của trẻ và chiếm thêm phương sai về sự lo lắng của trẻ, ngoài sự kiểm soát của cha mẹ. Có một tác động gián tiếp của sự lo lắng của cha mẹ đối với sự lo lắng của trẻ thông qua sự kiểm soát của cha mẹ và sự tránh né trải nghiệm của cha mẹ. Sự tránh né trải nghiệm của cha mẹ điều chỉnh mối quan hệ giữa sự kiểm soát của cha mẹ và sự lo lắng của trẻ, sao cho mối quan hệ chỉ có ý nghĩa ở mức độ tránh né trải nghiệm của cha mẹ cao. Nghiên cứu hiện tại cung cấp sự hỗ trợ cho vai trò của sự tránh né trải nghiệm của cha mẹ trong việc hiểu biết về sự lo lắng giữa các thế hệ. Nghiên cứu trong tương lai nên lặp lại nghiên cứu với một mẫu lâm sàng. Các ý nghĩa lý thuyết và thực hành được xem xét."} +{"idx": 1910020, "en": "Big data approaches to psychology have become increasing popular (Jones, 2017). Two of the main developments of this line of research is the advent of distributional models of semantics (e.g., Landauer and Dumais, 1997), which learn the meaning of words from large text corpora, and the collection of mega datasets of human behavior (e.g., The English lexicon project; Balota et al., 2007). The current article combines these two approaches, with the goal being to understand the consistency and preference that people have for word meanings. This was accomplished by mining a large amount of data from an online, crowdsourced dictionary and analyzing this data with a distributional model. Overall, it was found that even for words that are not an active part of the language environment, there is a large amount of consistency in the word meanings that different people have. Additionally, it was demonstrated that users of a language have strong preferences for word meanings, such that definitions to words that do not conform to people's conceptions are rejected by a community of language users. The results of this article provides insights into the cultural evolution of word meanings, and sheds light on alternative methodologies that can be used to understand lexical behavior.", "gemini": "Các phương pháp tiếp cận dữ liệu lớn đối với tâm lý học đã trở nên ngày càng phổ biến (Jones, 2017). Hai trong số những phát triển chính của hướng nghiên cứu này là sự ra đời của các mô hình ngữ nghĩa phân phối (ví dụ: Landauer và Dumais, 1997), học nghĩa của các từ từ các tập dữ liệu văn bản lớn và bộ sưu tập các tập dữ liệu lớn về hành vi của con người (ví dụ: Dự án từ vựng tiếng Anh; Balota et al., 2007). Bài báo hiện tại kết hợp hai cách tiếp cận này, với mục tiêu là hiểu được sự nhất quán và sở thích mà mọi người dành cho ý nghĩa của từ. Điều này được thực hiện bằng cách khai thác một lượng lớn dữ liệu từ một từ điển trực tuyến do cộng đồng cung cấp và phân tích dữ liệu này bằng một mô hình phân phối. Nhìn chung, người ta thấy rằng ngay cả đối với những từ không phải là một phần tích cực của môi trường ngôn ngữ, vẫn có một lượng lớn sự nhất quán trong ý nghĩa của từ mà những người khác nhau có. Ngoài ra, người ta đã chứng minh rằng những người sử dụng một ngôn ngữ có sở thích mạnh mẽ đối với ý nghĩa của từ, sao cho các định nghĩa đối với những từ không phù hợp với quan niệm của mọi người sẽ bị một cộng đồng người dùng ngôn ngữ từ chối. Kết quả của bài báo này cung cấp những hiểu biết sâu sắc về sự tiến hóa văn hóa của ý nghĩa từ ngữ và làm sáng tỏ các phương pháp luận thay thế có thể được sử dụng để hiểu hành vi từ vựng."} +{"idx": 1910021, "en": "Most interpreting theories claim that different interpreting types should involve varied processing mechanisms and procedures. However, few studies have examined their underlying differences. Even though some previous results based on quantitative approaches show that different interpreting types yield outputs of varying lexical and syntactic features, the grammatical parsing approach is limited. Language sequences that form without relying on parsing or processing with a specific linguistic approach or grammar excel other quantitative approaches at revealing the sequential behavior of language production. As a non-grammatically-bound unit of language sequences, frequency motif can visualize the local distribution of content and function words, and can also statistically classify languages and identify text types. Thus, the current research investigates the distribution, length and position-dependent properties of frequency motifs across different interpreting outputs in pursuit of the sequential generation behaviors. It is found that the distribution, the length and certain position-dependent properties of the specific language sequences differ significantly across simultaneous interpreting and consecutive interpreting output. The features of frequency motifs manifest that both interpreting output is produced in the manner that abides by the least effort principle. The current research suggests that interpreting types can be differentiated through this type of language sequential unit and offers evidence for how the different task features mediate the sequential organization of interpreting output under different demand to achieve cognitive load minimization.", "gemini": "Hầu hết các lý thuyết thông dịch đều cho rằng các loại thông dịch khác nhau cần có các cơ chế và quy trình xử lý khác nhau. Tuy nhiên, rất ít nghiên cứu xem xét sự khác biệt cơ bản của chúng. Mặc dù một số kết quả trước đây dựa trên các phương pháp định lượng cho thấy các loại thông dịch khác nhau tạo ra các đầu ra có các đặc điểm cú pháp và từ vựng khác nhau, nhưng phương pháp phân tích cú pháp ngữ pháp vẫn còn hạn chế. Các chuỗi ngôn ngữ được hình thành mà không dựa vào việc phân tích cú pháp hoặc xử lý bằng một phương pháp tiếp cận ngôn ngữ hoặc ngữ pháp cụ thể vượt trội hơn các phương pháp định lượng khác trong việc tiết lộ hành vi tuần tự của quá trình sản xuất ngôn ngữ. Là một đơn vị không bị ràng buộc về mặt ngữ pháp của các chuỗi ngôn ngữ, mô típ tần suất có thể trực quan hóa sự phân phối cục bộ của các từ nội dung và chức năng, đồng thời cũng có thể phân loại ngôn ngữ và xác định các loại văn bản một cách thống kê. Do đó, nghiên cứu hiện tại điều tra các đặc tính về phân phối, độ dài và vị trí của các mô típ tần suất trên các đầu ra thông dịch khác nhau nhằm theo đuổi các hành vi tạo chuỗi tuần tự. Người ta thấy rằng sự phân phối, độ dài và một số đặc tính phụ thuộc vào vị trí của các chuỗi ngôn ngữ cụ thể khác nhau đáng kể giữa đầu ra thông dịch đồng thời và đầu ra thông dịch tuần tự. Các đặc điểm của các mô típ tần suất biểu hiện rằng cả hai đầu ra thông dịch đều được tạo ra theo cách tuân theo nguyên tắc nỗ lực ít nhất. Nghiên cứu hiện tại cho thấy các loại thông dịch có thể được phân biệt thông qua loại đơn vị tuần tự ngôn ngữ này và đưa ra bằng chứng về cách các đặc điểm nhiệm vụ khác nhau làm trung gian cho tổ chức tuần tự của đầu ra thông dịch theo nhu cầu khác nhau để đạt được mục tiêu giảm thiểu tải nhận thức."} +{"idx": 1910022, "en": "Job insecurity is a growing phenomenon, typical of an employment context characterised by high rates of temporary work and unemployment. Previous research has shown a direct relationship between job insecurity and mental health impairment. The present analysis goes into this relationship in depth, studying the moderating role of coping strategies and predicting that men and women implement different types of strategies. A sample of 1.008 workers is analysed, 588 women and 420 men. The Tobin CSI scale was used to analyse the coping strategies, in addition to JIS-8 to assess job insecurity, the MOS Perceived Social Support Survey and the GHQ-28 test to evaluate mental health. Then, a hierarchical linear regression was designed to study the moderating role of 8 coping strategies of job insecurity and 4 mental health subscales in men and women, separately. Results illustrate that coping strategies play a moderating role in the relationship between job insecurity and mental health. However, the aggravating role of disengagement coping strategies is more relevant than the buffering role of engagement strategies. On the other hand, women implement a greater number of coping strategies, with more positive results for mental health. Also, in the relationship between job insecurity and mental health the most important strategies are the ones related to social interaction inside and outside an organisation, and these are the main ones used by women. It therefore follows that strengthening rich social relationships inside and outside the working environment is a guarantee of well-being.", "gemini": "Sự bất ổn định trong công việc là một hiện tượng đang gia tăng, đặc trưng của bối cảnh việc làm có tỷ lệ công việc tạm thời và thất nghiệp cao. Các nghiên cứu trước đây đã chỉ ra mối quan hệ trực tiếp giữa sự bất ổn định trong công việc và suy giảm sức khỏe tâm thần. Phân tích hiện tại đi sâu vào mối quan hệ này, nghiên cứu vai trò điều chỉnh của các chiến lược đối phó và dự đoán rằng nam giới và phụ nữ thực hiện các loại chiến lược khác nhau. Một mẫu gồm 1.008 người lao động được phân tích, 588 phụ nữ và 420 nam giới. Thang Tobin CSI được sử dụng để phân tích các chiến lược đối phó, ngoài JIS-8 để đánh giá sự bất ổn định trong công việc, Khảo sát hỗ trợ xã hội được nhận thức của MOS và bài kiểm tra GHQ-28 để đánh giá sức khỏe tâm thần. Sau đó, một hồi quy tuyến tính phân cấp được thiết kế để nghiên cứu vai trò điều chỉnh của 8 chiến lược đối phó với sự bất ổn định trong công việc và 4 thang phụ sức khỏe tâm thần ở nam và nữ, riêng biệt. Kết quả minh họa rằng các chiến lược đối phó đóng vai trò điều chỉnh trong mối quan hệ giữa sự bất ổn định trong công việc và sức khỏe tâm thần. Tuy nhiên, vai trò làm trầm trọng thêm của các chiến lược đối phó không tham gia có liên quan hơn đến vai trò đệm của các chiến lược tham gia. Mặt khác, phụ nữ thực hiện nhiều chiến lược đối phó hơn, với kết quả tích cực hơn đối với sức khỏe tâm thần. Ngoài ra, trong mối quan hệ giữa sự bất ổn định trong công việc và sức khỏe tâm thần, các chiến lược quan trọng nhất là những chiến lược liên quan đến tương tác xã hội trong và ngoài tổ chức, và đây là những chiến lược chính được phụ nữ sử dụng. Do đó, việc củng cố các mối quan hệ xã hội phong phú trong và ngoài môi trường làm việc là sự đảm bảo cho sức khỏe."} +{"idx": 1910023, "en": "Background: Previous studies on the effectiveness of psychological interventions in oncology mainly used quantitative measures and no study was conducted with regard to both caregivers and patients. Aim: This study evaluates the effectiveness of psychoeducational support groups, both for women with breast cancer, and for their informal caregivers through the use of quantitative and qualitative measures. Methods: A longitudinal design was used comparing two psychoeducational support groups with other two groups in a standard care control condition. Participants were 28 women with a diagnosis of breast cancer in the care of a hospital in Northern Italy, and 21 family caregivers. The quantitative data were collected by Cognitive Behavioral Assessment for Outcome Evaluation (CBA-OE) and the qualitative data through the use of semi-structured interviews. Results: The statistical analysis showed a significant change attributable to the psychological intervention that proves the effectiveness of such an intervention in the patients' and caregivers' group. The qualitative analysis allowed us to interpret the behavioral and psychological profile emerging from CBA-OE, by considering the subjective experience of the treatment groups. The group experience offered affective, relational and informative support, and allowed participants to create a network and to feel understood and reassured. Conclusion: The results suggest the usefulness of psychoeducational support groups for women with breast cancer and for their caregivers. The value of this kind of intervention is not only at an individual level but also at a systems level, and family involvement ensures the best positive outcomes.", "gemini": "Bối cảnh: Các nghiên cứu trước đây về hiệu quả của các can thiệp tâm lý trong ung thư chủ yếu sử dụng các biện pháp định lượng và không có nghiên cứu nào được tiến hành đối với cả người chăm sóc và bệnh nhân. Mục đích: Nghiên cứu này đánh giá hiệu quả của các nhóm hỗ trợ giáo dục tâm lý, dành cho cả phụ nữ bị ung thư vú và cho những người chăm sóc không chính thức của họ thông qua việc sử dụng các biện pháp định lượng và định tính. Phương pháp: Một thiết kế theo chiều dọc đã được sử dụng để so sánh hai nhóm hỗ trợ giáo dục tâm lý với hai nhóm khác trong điều kiện kiểm soát chăm sóc tiêu chuẩn. Những người tham gia là 28 phụ nữ được chẩn đoán mắc bệnh ung thư vú đang được chăm sóc tại một bệnh viện ở miền Bắc nước Ý và 21 người chăm sóc gia đình. Dữ liệu định lượng được thu thập bằng Đánh giá hành vi nhận thức để đánh giá kết quả (CBA-OE) và dữ liệu định tính thông qua việc sử dụng các cuộc phỏng vấn bán cấu trúc. Kết quả: Phân tích thống kê cho thấy một sự thay đổi đáng kể có thể quy cho can thiệp tâm lý, chứng minh hiệu quả của can thiệp như vậy trong nhóm bệnh nhân và người chăm sóc. Phân tích định tính cho phép chúng tôi diễn giải hồ sơ hành vi và tâm lý xuất hiện từ CBA-OE, bằng cách xem xét trải nghiệm chủ quan của các nhóm điều trị. Trải nghiệm của nhóm cung cấp sự hỗ trợ về tình cảm, quan hệ và thông tin, đồng thời cho phép những người tham gia tạo ra một mạng lưới và cảm thấy được hiểu và an tâm. Kết luận: Kết quả cho thấy tính hữu ích của các nhóm hỗ trợ giáo dục tâm lý cho phụ nữ bị ung thư vú và những người chăm sóc họ. Giá trị của loại can thiệp này không chỉ ở cấp độ cá nhân mà còn ở cấp độ hệ thống và sự tham gia của gia đình đảm bảo kết quả tích cực nhất."} +{"idx": 1910024, "en": "The aim of the present study was to identify sub-types of moped riders based on a cluster analysis of specific personality characteristics (i.e., driving anger, anxiety, angry hostility, excitement-seeking, altruism, normlessness, and driving locus of control) within a large sample of Italian adolescents. The study had also the aim to compare the emerged sub-types of moped riders on measures of attitudes toward safe driving, risky driving behaviors (e.g., rule's violations and speeding, not using helmet, drinking and driving, etc.), and self-reported tickets and accident involvement. One thousand two hundred seventy-three Italian high school students aged from 13 to 19 years (meanage = 15.43, SD = 0.98) with a valid driving license for moped participated to the study. Results revealed three sub-types of moped riders (namely risky, worried and careful moped riders), which differ significantly for risky driving behaviors, attitudes toward traffic safety, risk perception, and self-reported accident involvement. Importantly, the results of the present study showed that the personality and behavioral characteristics of the three sub-groups of moped riders substantially resembled those identified by previous studies with vehicle drivers of different ages; thus, empirically supporting the notion that certain combinations of personality characteristics are associated with risk driving tendencies and behaviors in both young moped riders and car drivers. Safe driving interventions can tackle risky driving beliefs and behavioral tendencies in young moped riders and car drivers by tailoring their messages according to the personality sub-types of the target groups.", "gemini": "Mục đích của nghiên cứu hiện tại là xác định các phân nhóm người đi xe gắn máy dựa trên phân tích cụm các đặc điểm tính cách cụ thể (tức là tức giận khi lái xe, lo lắng, thù địch tức giận, tìm kiếm sự phấn khích, vị tha, vô chuẩn mực và nơi kiểm soát khi lái xe) trong một nhóm lớn thanh thiếu niên Ý. Nghiên cứu cũng có mục đích so sánh các phân nhóm người đi xe gắn máy mới nổi về các biện pháp thái độ đối với việc lái xe an toàn, hành vi lái xe mạo hiểm (ví dụ: vi phạm luật và chạy quá tốc độ, không đội mũ bảo hiểm, uống rượu và lái xe, v.v.) và các vé tự báo cáo và liên quan đến tai nạn. Một nghìn hai trăm bảy mươi ba học sinh trung học Ý trong độ tuổi từ 13 đến 19 (tuổi trung bình = 15,43, SD = 0,98) có giấy phép lái xe gắn máy hợp lệ đã tham gia nghiên cứu. Kết quả cho thấy ba phân nhóm người đi xe gắn máy (tức là người đi xe gắn máy mạo hiểm, lo lắng và cẩn thận), có sự khác biệt đáng kể về hành vi lái xe mạo hiểm, thái độ đối với an toàn giao thông, nhận thức rủi ro và tự báo cáo liên quan đến tai nạn. Điều quan trọng là kết quả của nghiên cứu hiện tại cho thấy rằng các đặc điểm tính cách và hành vi của ba phân nhóm người đi xe gắn máy về cơ bản giống với những đặc điểm đã được xác định bởi các nghiên cứu trước đây với những người lái xe ở các độ tuổi khác nhau; do đó, hỗ trợ thực nghiệm cho quan điểm rằng một số kết hợp các đặc điểm tính cách có liên quan đến xu hướng và hành vi lái xe mạo hiểm ở cả người đi xe gắn máy trẻ và người lái ô tô. Các biện pháp can thiệp lái xe an toàn có thể giải quyết các niềm tin lái xe mạo hiểm và xu hướng hành vi ở những người đi xe gắn máy trẻ và người lái ô tô bằng cách điều chỉnh thông điệp của họ theo các phân nhóm tính cách của các nhóm mục tiêu."} +{"idx": 1910025, "en": "This study examines the relationship between corporate social responsibility (CSR) perception and emotional labor strategies, and the effects of the interaction between CSR perception and moral identification on emotional labor strategies via affective organizational commitment. Our data from 352 frontline employees in the service industry show the main effect of CSR perception on emotional labor strategies. We find that CSR perception is positively (negatively) related to deep acting (surface acting). Affective organizational commitment mediates the relationship between CSR perception and surface acting but not between CSR perception and deep acting. Moral identification moderates the effects of CSR perception on surface acting through affective organizational commitment. This paper reveals that the employees' views on their organization's social responsiveness and morality affect their emotional labor strategies.", "gemini": "Nghiên cứu này xem xét mối quan hệ giữa nhận thức về trách nhiệm xã hội của doanh nghiệp (CSR) và các chiến lược lao động cảm xúc, cũng như các tác động của sự tương tác giữa nhận thức về CSR và nhận dạng đạo đức đối với các chiến lược lao động cảm xúc thông qua cam kết tổ chức có tình cảm. Dữ liệu của chúng tôi từ 352 nhân viên tuyến đầu trong ngành dịch vụ cho thấy tác động chính của nhận thức về CSR đối với các chiến lược lao động cảm xúc. Chúng tôi thấy rằng nhận thức về CSR có mối quan hệ tích cực (tiêu cực) với hành động sâu sắc (diễn xuất bề ngoài). Cam kết tổ chức có tình cảm đóng vai trò trung gian trong mối quan hệ giữa nhận thức về CSR và hành động bề ngoài nhưng không phải giữa nhận thức về CSR và hành động sâu sắc. Nhận dạng đạo đức điều chỉnh các tác động của nhận thức về CSR đối với hành động bề ngoài thông qua cam kết tổ chức có tình cảm. Bài báo này tiết lộ rằng quan điểm của nhân viên về khả năng phản ứng xã hội và đạo đức của tổ chức của họ ảnh hưởng đến các chiến lược lao động cảm xúc của họ."} +{"idx": 1910026, "en": "Experimental investigations into children's interpretation of scalar terms show that children have difficulties with scalar implicatures in tasks. In contrast with adults, they are for instance not able to derive the pragmatic interpretation that \"some\" means \"not all\" (Noveck, 2001; Papafragou and Musolino, 2003). However, there is also substantial experimental evidence that children are not incapable of drawing scalar inferences and that they are aware of the pragmatic potential of scalar expressions. In these kinds of studies, the prime interest is to discover what conditions facilitate implicature production for children. One of the factors that seem to be difficult for children is the generation of the scalar alternative. In a Felicity Judgment Task (FJT) the alternative is given. Participants are presented with a pair of utterances and asked to choose the most felicitous description. In such a task, even 5-year-old children are reported to show a very good performance. Our study wants to build on this tradition, by using a FJT where not only \"some-all\" choices are given, but also \"some-many\" and \"many-all.\" In combination with a manipulation of the number of successes/failures in the stories, this enabled us to construct control, critical and ambiguous items. We compared the performance of 59 5-year-old children with that of 34 11-year-old children. The results indicated that performance of both age groups was clearly above chance, replicating previous findings. However, for the 5-year-old children, the critical and ambiguous items were more difficult than the control items and they also performed worse on these two types of items than the 11-year-old children. Interestingly with respect to the issue of scalar diversity, the 11-year-old children were also presented temporal items, which turned out to be more difficult than the quantitative ones.", "gemini": "Các cuộc điều tra thực nghiệm về cách trẻ em diễn giải các thuật ngữ thang đo cho thấy trẻ em gặp khó khăn với các hàm ý thang đo trong các nhiệm vụ. Ngược lại với người lớn, chẳng hạn, chúng không thể đưa ra cách diễn giải thực dụng rằng \"một số\" có nghĩa là \"không phải tất cả\" (Noveck, 2001; Papafragou và Musolino, 2003). Tuy nhiên, cũng có bằng chứng thực nghiệm đáng kể cho thấy trẻ em không mất khả năng rút ra suy luận thang đo và chúng nhận thức được tiềm năng thực dụng của các biểu thức thang đo. Trong các loại nghiên cứu này, mối quan tâm chính là khám phá những điều kiện nào tạo điều kiện thuận lợi cho việc tạo ra hàm ý đối với trẻ em. Một trong những yếu tố dường như khó khăn đối với trẻ em là tạo ra phương án thay thế thang đo. Trong Nhiệm vụ phán đoán hạnh phúc (FJT), phương án thay thế được đưa ra. Người tham gia được trình bày một cặp câu nói và được yêu cầu chọn mô tả phù hợp nhất. Trong một nhiệm vụ như vậy, thậm chí trẻ em 5 tuổi cũng được báo cáo là có thành tích rất tốt. Nghiên cứu của chúng tôi muốn xây dựng dựa trên truyền thống này, bằng cách sử dụng FJT, trong đó không chỉ đưa ra các lựa chọn \"một số-tất cả\", mà còn đưa ra \"một số-nhiều\" và \"nhiều-tất cả\". Kết hợp với việc thao túng số lần thành công/thất bại trong các câu chuyện, điều này cho phép chúng tôi xây dựng các mục kiểm soát, quan trọng và mơ hồ. Chúng tôi đã so sánh thành tích của 59 trẻ em 5 tuổi với 34 trẻ em 11 tuổi. Kết quả cho thấy thành tích của cả hai nhóm tuổi đều rõ ràng cao hơn ngẫu nhiên, sao chép các phát hiện trước đó. Tuy nhiên, đối với trẻ em 5 tuổi, các mục quan trọng và mơ hồ khó hơn các mục kiểm soát và chúng cũng thực hiện kém hơn ở hai loại mục này so với trẻ em 11 tuổi. Điều thú vị là liên quan đến vấn đề đa dạng thang đo, trẻ em 11 tuổi cũng được trình bày các mục thời gian, hóa ra khó hơn các mục định lượng."} +{"idx": 1910027, "en": "Improved understanding of neuroimaging signal changes and their relation to patient outcomes after ischemic stroke is needed to improve ability to predict motor improvement and make therapy recommendations. The posterior limb of the internal capsule (PLIC) is a hub of afferent and efferent motor signaling and this work proposes new, image-based methods for prognosis based on interhemispheric differences in the PLIC. In this work, nine acute supratentorial ischemic stroke patients with motor impairment received a baseline, 203-direction diffusion brain MRI and a clinical assessment 3-12 days post-stroke and were compared to nine age-matched healthy controls. Asymmetries based on the mean and Kullback-Leibler divergence in the ipsilesional and contralesional PLIC were calculated for diffusion tensor imaging (DTI) and diffusion spectrum imaging (DSI) measures from the baseline MRI. Predictions of upper extremity Fugl-Meyer (FM) scores at 5-weeks follow-up from baseline measures of PLIC asymmetry in diffusion tensor imaging (DTI) and diffusion spectrum imaging (DSI) models were evaluated. For the stroke participants, the baseline asymmetry measures in the PLIC for the orientation dispersion index of the neurite orientation dispersion and density imaging (NODDI) model were highly correlated with upper extremity FM outcomes (r 2 = 0.83). Use of DSI and the NODDI orientation dispersion index parameter shows promise of being more predictive of stroke recovery and to help better understand white matter changes in stroke, beyond DTI measures. The new finding that baseline interhemispheric differences in the PLIC calculated from the orientation dispersion index of the NODDI model are highly correlated with upper extremity functional outcomes may lead to improved image-based motor-outcome prediction after middle cerebral artery ischemic stroke.", "gemini": "Cần cải thiện sự hiểu biết về những thay đổi tín hiệu hình ảnh thần kinh và mối quan hệ của chúng với kết quả của bệnh nhân sau đột quỵ thiếu máu cục bộ để cải thiện khả năng dự đoán sự cải thiện vận động và đưa ra khuyến nghị về liệu pháp. Chi sau của bao trong là trung tâm của tín hiệu vận động hướng tâm và hướng ly tâm và công trình này đề xuất các phương pháp mới dựa trên hình ảnh để tiên lượng dựa trên sự khác biệt giữa hai bán cầu trong PLIC. Trong công trình này, chín bệnh nhân đột quỵ thiếu máu cục bộ cấp tính trên lều với suy giảm vận động đã được chụp MRI não khuếch tán 203 hướng ban đầu và đánh giá lâm sàng sau đột quỵ từ 3-12 ngày và được so sánh với chín đối chứng khỏe mạnh có độ tuổi phù hợp. Sự bất đối xứng dựa trên độ lệch Kullback-Leibler trung bình và trong PLIC tổn thương và đối diện tổn thương được tính toán cho hình ảnh tensor khuếch tán (DTI) và các phép đo hình ảnh phổ khuếch tán (DSI) từ MRI ban đầu. Các dự đoán về điểm Fugl-Meyer (FM) của chi trên sau 5 tuần theo dõi từ các phép đo ban đầu về sự bất đối xứng PLIC trong các mô hình hình ảnh tensor khuếch tán (DTI) và hình ảnh phổ khuếch tán (DSI) đã được đánh giá. Đối với những người tham gia đột quỵ, các phép đo bất đối xứng ban đầu ở PLIC đối với chỉ số phân tán hướng của mô hình hình ảnh mật độ phân tán và định hướng sợi trục thần kinh (NODDI) có mối tương quan cao với kết quả FM chi trên (r 2 = 0,83). Việc sử dụng DSI và thông số chỉ số phân tán hướng NODDI cho thấy hứa hẹn có thể dự đoán tốt hơn về khả năng phục hồi sau đột quỵ và giúp hiểu rõ hơn về những thay đổi chất trắng trong đột quỵ, vượt ra ngoài các phép đo DTI. Phát hiện mới cho thấy sự khác biệt giữa hai bán cầu ban đầu ở PLIC được tính toán từ chỉ số phân tán hướng của mô hình NODDI có mối tương quan cao với kết quả chức năng chi trên có thể dẫn đến cải thiện dự đoán kết quả vận động dựa trên hình ảnh sau đột quỵ thiếu máu cục bộ động mạch não giữa."} +{"idx": 1910028, "en": "Gonadotropins are essential for reproduction control in humans as well as in animals. They are widely used all over the world for ovarian stimulation in women, spermatogenesis stimulation in men, and ovulation induction and superovulation in animals. Despite the availability of many different preparations, all are made of the native hormones. Having different ligands with a wide activity range for a given receptor helps better understand its molecular and cellular signaling mechanisms as well as its physiological functions, and thus helps the development of more specific and adapted medicines. One way to control the gonadotropins' activity could be the use of modulating antibodies. Antibodies are powerful tools that were largely used to decipher gonadotropins' actions and they have shown their utility as therapeutics in several other indications such as cancer. In this review, we summarize the inhibitory and potentiating antibodies to gonadotropins, and their potential therapeutic applications.", "gemini": "Gonadotropin rất cần thiết cho việc kiểm soát sinh sản ở cả người và động vật. Chúng được sử dụng rộng rãi trên toàn thế giới để kích thích buồng trứng ở phụ nữ, kích thích sinh tinh ở nam giới và gây rụng trứng và rụng trứng quá mức ở động vật. Mặc dù có nhiều chế phẩm khác nhau, tất cả đều được tạo thành từ các hormone tự nhiên. Có các phối tử khác nhau với phạm vi hoạt động rộng đối với một thụ thể nhất định giúp hiểu rõ hơn về cơ chế truyền tín hiệu phân tử và tế bào cũng như chức năng sinh lý của nó, do đó giúp phát triển các loại thuốc đặc hiệu và thích ứng hơn. Một cách để kiểm soát hoạt động của gonadotropin có thể là sử dụng các kháng thể điều biến. Kháng thể là những công cụ mạnh mẽ được sử dụng rộng rãi để giải mã các hoạt động của gonadotropin và chúng đã chứng minh được tính hữu ích của mình như một liệu pháp điều trị trong một số chỉ định khác như ung thư. Trong bài đánh giá này, chúng tôi tóm tắt các kháng thể ức chế và làm tăng tác dụng đối với gonadotropin và các ứng dụng điều trị tiềm năng của chúng."} +{"idx": 1910029, "en": "Objective: This study aimed to test associations between type 2 diabetes mellitus (T2DM) and metabolites in urea cycle including arginine, citrulline and ornithine. Methods:This study used a hospital-based cross-sectional study design. We retrieved medical notes of 401 in-patients with onset of T2DM within 2 years and 1,522 healthy subjects who attended annual physical examination. All cases were admitted to a tertiary care center in Jinzhou, China from May 2015 to August 2016. Binary logistic regression analyses were performed to obtain odds ratios (ORs) and 95% confidence intervals (CIs). Results:Patients with T2DM had higher arginine, and lower ornithine than control subjects. Levels of citrulline were similar in two groups. Arginine was positively associated with T2DM (ORs: 1.20, 1.17-1.23) while ornithine was negatively associated with T2DM (OR: 0.89, 0.88-0.91). After adjustment for other amino acids and traditional risk factors, these associations were still significant and persistent for arginine and ornithine. The association between citrulline and T2DM was not significant. Their ratios of pairs of two amino acids were associated with increased risk of T2DM. After adjustment for other ratios of amino acids, effect size for T2DM remained significant. Further adjustment for traditional risk factors did not lead to large changes (ORs: 1.78, 1.20-2.65 for the ratio of arginine to ornithine; ORs: 1.59, 1.37-1.86 for the ratio of citrulline to ornithine, respectively) except the ratio of arginine to citrulline. Conclusions: Plasma levels of amino acids related to urea cycle and their ratios of these amino-acids were associated with T2DM in Chinese adults.", "gemini": "Mục tiêu: Nghiên cứu này nhằm mục đích kiểm tra mối liên quan giữa bệnh đái tháo đường týp 2 (T2DM) và các chất chuyển hóa trong chu trình urê bao gồm arginine, citrulline và ornithine. Phương pháp: Nghiên cứu này sử dụng thiết kế nghiên cứu cắt ngang tại bệnh viện. Chúng tôi đã lấy lại hồ sơ bệnh án của 401 bệnh nhân nội trú mắc T2DM trong vòng 2 năm và 1.522 đối tượng khỏe mạnh tham gia khám sức khỏe định kỳ. Tất cả các trường hợp đều được đưa vào một trung tâm chăm sóc sức khỏe cấp ba ở Cẩm Châu, Trung Quốc từ tháng 5 năm 2015 đến tháng 8 năm 2016. Phân tích hồi quy logistic nhị phân được thực hiện để có được tỷ lệ cược (OR) và khoảng tin cậy 95% (CI). Kết quả: Bệnh nhân mắc T2DM có nồng độ arginine cao hơn và ornithine thấp hơn so với đối tượng chứng. Nồng độ citrulline tương tự nhau ở hai nhóm. Arginine có liên quan tích cực với T2DM (OR: 1,20, 1,17-1,23) trong khi ornithine có liên quan tiêu cực với T2DM (OR: 0,89, 0,88-0,91). Sau khi điều chỉnh các axit amin khác và các yếu tố nguy cơ truyền thống, những mối liên quan này vẫn có ý nghĩa và liên tục đối với arginine và ornithine. Mối liên quan giữa citrulline và T2DM không đáng kể. Tỷ lệ của cặp hai axit amin có liên quan đến việc tăng nguy cơ mắc T2DM. Sau khi điều chỉnh các tỷ lệ khác của axit amin, kích thước hiệu ứng đối với T2DM vẫn có ý nghĩa. Việc điều chỉnh thêm các yếu tố nguy cơ truyền thống không dẫn đến những thay đổi lớn (OR: 1,78, 1,20-2,65 đối với tỷ lệ arginine/ornithine; OR: 1,59, 1,37-1,86 đối với tỷ lệ citrulline/ornithine, tương ứng) ngoại trừ tỷ lệ arginine/citrulline. Kết luận: Nồng độ axit amin trong huyết tương liên quan đến chu trình urê và tỷ lệ của các axit amin này có liên quan đến T2DM ở người lớn Trung Quốc."} +{"idx": 1910030, "en": "Candidatus Accumulibacter phosphatis is in general presented as the dominant organism responsible for the biological removal of phosphorus in activated sludge wastewater treatment plants. Lab-scale enhanced biological phosphorus removal (EBPR) studies, usually use acetate as carbon source. However, the complexity of the carbon sources present in wastewater could allow other potential poly-phosphate accumulating organism (PAOs), such as putative fermentative PAOs (e.g., Tetrasphaera), to proliferate in coexistence or competition with Ca. Accumulibacter. This research assessed the effects of lactate on microbial selection and process performance of an EBPR lab-scale study. The addition of lactate resulted in the coexistence of Ca. Accumulibacter and Tetrasphaera in a single EBPR reactor. An increase in anaerobic glycogen consumption from 1.17 to 2.96 C-mol/L and anaerobic PHV formation from 0.44 to 0.87 PHV/PHA C-mol/C-mol corresponded to the increase in the influent lactate concentration. The dominant metabolism shifted from a polyphosphate-accumulating metabolism (PAM) to a glycogen accumulating metabolism (GAM) without EBPR activity. However, despite the GAM, traditional glycogen accumulating organisms (GAOs; Candidatus Competibacter phosphatis and Defluvicoccus) were not detected. Instead, the 16s RNA amplicon analysis showed that the genera Tetrasphaera was the dominant organism, while a quantification based on FISH-biovolume indicated that Ca. Accumulibacter remained the dominant organism, indicating certain discrepancies between these microbial analytical methods. Despite the discrepancies between these microbial analytical methods, neither Ca. Accumulibacter nor Tetrasphaera performed biological phosphorus removal by utilizing lactate as carbon source.", "gemini": "Candidatus Accumulibacter phosphatis thường được coi là sinh vật chiếm ưu thế chịu trách nhiệm loại bỏ sinh học phốt pho trong các nhà máy xử lý nước thải bùn hoạt tính. Các nghiên cứu loại bỏ phốt pho sinh học tăng cường (EBPR) ở quy mô phòng thí nghiệm thường sử dụng axetat làm nguồn cacbon. Tuy nhiên, sự phức tạp của các nguồn cacbon có trong nước thải có thể cho phép các sinh vật tích lũy poly-photphat tiềm năng khác (PAO), chẳng hạn như PAO lên men giả định (ví dụ: Tetrasphaera), phát triển mạnh cùng tồn tại hoặc cạnh tranh với Ca. Accumulibacter. Nghiên cứu này đánh giá tác động của lactat đối với quá trình lựa chọn vi sinh vật và hiệu suất quy trình của một nghiên cứu EBPR quy mô phòng thí nghiệm. Việc bổ sung lactat dẫn đến sự cùng tồn tại của Ca. Accumulibacter và Tetrasphaera trong một lò phản ứng EBPR duy nhất. Sự gia tăng tiêu thụ glycogen kỵ khí từ 1,17 đến 2,96 C-mol/L và sự hình thành PHV kỵ khí từ 0,44 đến 0,87 PHV/PHA C-mol/C-mol tương ứng với sự gia tăng nồng độ lactat trong nước vào. Quá trình trao đổi chất chiếm ưu thế chuyển từ quá trình trao đổi chất tích lũy polyphosphat (PAM) sang quá trình trao đổi chất tích lũy glycogen (GAM) mà không có hoạt động EBPR. Tuy nhiên, mặc dù có GAM, các sinh vật tích lũy glycogen truyền thống (GAO; Candidatus Competibacter phosphatis và Defluvicoccus) không được phát hiện. Thay vào đó, phân tích amplicon RNA 16s cho thấy chi Tetrasphaera là sinh vật chiếm ưu thế, trong khi định lượng dựa trên thể tích sinh học FISH chỉ ra rằng Ca. Accumulibacter vẫn là sinh vật chiếm ưu thế, cho thấy sự khác biệt nhất định giữa các phương pháp phân tích vi sinh vật này. Mặc dù có sự khác biệt giữa các phương pháp phân tích vi sinh vật này, nhưng cả Ca. Accumulibacter cũng như Tetrasphaera đều không thực hiện loại bỏ phốt pho sinh học bằng cách sử dụng lactat làm nguồn cacbon."} +{"idx": 1910031, "en": "The discovery of receptor-receptor interactions (RRI) has expanded our understanding of the role that G protein-coupled receptors (GPCRs) play in intercellular communication. The finding that GPCRs can operate as receptor complexes, and not only as monomers, suggests that several different incoming signals could already be integrated at the plasma membrane level via direct allosteric interactions between the protomers that form the complex. Most research in this field has focused on neuronal populations and has led to the identification of a large number of RRI. However, RRI have been seen to occur not only in neurons but also in astrocytes and, outside the central nervous system, in cells of the cardiovascular and endocrine systems and in cancer cells. Furthermore, RRI involving the formation of macromolecular complexes are not limited to GPCRs, being also observed in other families of receptors. Thus, RRI appear as a widespread phenomenon and oligomerization as a common mechanism for receptor function and regulation. The discovery of these macromolecular assemblies may well have a major impact on pharmacology. Indeed, the formation of receptor complexes significantly broadens the spectrum of mechanisms available to receptors for recognition and signaling, which may be implemented through modulation of the binding sites of the adjacent protomers and of their signal transduction features. In this context, the possible appearance of novel allosteric sites in the receptor complex structure may be of particular relevance. Thus, the existence of RRI offers the possibility of new therapeutic approaches, and novel pharmacological strategies for disease treatment have already been proposed. Several challenges, however, remain. These include the accurate characterization of the role that the receptor complexes identified so far play in pathological conditions and the development of ligands specific to given receptor complexes, in order to efficiently exploit the pharmacological properties of these complexes.", "gemini": "Việc phát hiện ra các tương tác thụ thể-thụ thể (RRI) đã mở rộng hiểu biết của chúng ta về vai trò của các thụ thể liên kết protein G (GPCR) trong giao tiếp tế bào. Phát hiện rằng GPCR có thể hoạt động như các phức hợp thụ thể, chứ không chỉ như các monome, cho thấy rằng một số tín hiệu đầu vào khác nhau có thể đã được tích hợp ở mức màng plasma thông qua các tương tác dị lập trực tiếp giữa các đơn vị tiền thân tạo nên phức hợp. Hầu hết các nghiên cứu trong lĩnh vực này tập trung vào quần thể nơ-ron và đã dẫn đến việc xác định được một số lượng lớn RRI. Tuy nhiên, RRI đã được thấy xảy ra không chỉ ở tế bào thần kinh mà còn ở tế bào hình sao và ngoài hệ thần kinh trung ương, ở các tế bào của hệ tim mạch và nội tiết và ở các tế bào ung thư. Hơn nữa, RRI liên quan đến sự hình thành các phức hợp đại phân tử không chỉ giới hạn ở GPCR mà còn được quan sát thấy ở các họ thụ thể khác. Do đó, RRI xuất hiện như một hiện tượng phổ biến và quá trình oligome hóa như một cơ chế chung cho chức năng và điều hòa thụ thể. Việc phát hiện ra các tập hợp đại phân tử này cũng có thể có tác động lớn đến dược lý. Thật vậy, sự hình thành các phức hợp thụ thể mở rộng đáng kể phổ các cơ chế có sẵn cho các thụ thể để nhận dạng và truyền tín hiệu, có thể được thực hiện thông qua điều chế các vị trí liên kết của các đơn vị tiền thân liền kề và các đặc điểm truyền tín hiệu của chúng. Trong bối cảnh này, sự xuất hiện có thể xảy ra của các vị trí dị lập mới trong cấu trúc phức hợp thụ thể có thể có liên quan đặc biệt. Do đó, sự tồn tại của RRI mang lại khả năng tiếp cận các phương pháp điều trị mới và các chiến lược dược lý mới để điều trị bệnh đã được đề xuất. Tuy nhiên, vẫn còn một số thách thức. Chúng bao gồm việc mô tả chính xác vai trò của các phức hợp thụ thể được xác định cho đến nay trong các tình trạng bệnh lý và sự phát triển các phối tử đặc hiệu cho các phức hợp thụ thể nhất định, để khai thác hiệu quả các đặc tính dược lý của các phức hợp này."} +{"idx": 1910032, "en": "Production of fuels, therapeutic drugs, chemicals, and biomaterials using sustainable biological processes have received renewed attention due to increasing environmental concerns. Despite having high industrial output, most of the current chemical processes are associated with environmentally undesirable by-products which escalate the cost of downstream processing. Compared to chemical processes, whole cell biocatalysts offer several advantages including high selectivity, catalytic efficiency, milder operational conditions and low impact on the environment, making this approach the current choice for synthesis and manufacturing of different industrial products. In this review, we present the application of whole cell actinobacteria for the synthesis of biologically active compounds, biofuel production and conversion of harmful compounds to less toxic by-products. Actinobacteria alone are responsible for the production of nearly half of the documented biologically active metabolites and many enzymes; with the involvement of various species of whole cell actinobacteria such as Rhodococcus, Streptomyces, Nocardia and Corynebacterium for the production of useful industrial commodities.", "gemini": "Việc sản xuất nhiên liệu, thuốc điều trị, hóa chất và vật liệu sinh học bằng các quy trình sinh học bền vững đã nhận được sự quan tâm trở lại do những lo ngại ngày càng gia tăng về môi trường. Mặc dù có sản lượng công nghiệp cao, nhưng hầu hết các quy trình hóa học hiện tại đều liên quan đến các sản phẩm phụ không mong muốn đối với môi trường, làm tăng chi phí xử lý hạ nguồn. So với các quy trình hóa học, các chất xúc tác sinh học toàn tế bào mang lại một số lợi thế bao gồm tính chọn lọc cao, hiệu quả xúc tác, điều kiện vận hành nhẹ hơn và tác động thấp đến môi trường, khiến phương pháp này trở thành lựa chọn hiện tại để tổng hợp và sản xuất các sản phẩm công nghiệp khác nhau. Trong bài đánh giá này, chúng tôi trình bày ứng dụng của toàn bộ vi khuẩn dạng sợi để tổng hợp các hợp chất hoạt tính sinh học, sản xuất nhiên liệu sinh học và chuyển đổi các hợp chất có hại thành các sản phẩm phụ ít độc hơn. Chỉ riêng vi khuẩn dạng sợi đã chịu trách nhiệm sản xuất gần một nửa số chất chuyển hóa hoạt tính sinh học được ghi nhận và nhiều loại enzyme; với sự tham gia của nhiều loài vi khuẩn dạng sợi toàn tế bào như Rhodococcus, Streptomyces, Nocardia và Corynebacterium để sản xuất các mặt hàng công nghiệp hữu ích."} +{"idx": 1910033, "en": "Microbial communities are essential for the maintenance and functioning of ecosystems, including fermented food ecosystems. The analysis of food microbial communities is mainly focused on lactic acid bacteria (LAB), while yeast diversity is less understood. Here, we describe the fungal diversity of a typical food fermented product, sourdough bread. The species diversity of 14 sourdoughs collected from bakeries located all over France was analyzed. Bakeries were chosen to represent diverse bakery practices and included bakers and farmer-bakers. Both non-culture-based (pyrosequencing of Internal Transcribed Spacer 1 amplicons) and culture-based methods were used. While both identification methods were in agreement regarding the dominant yeast species of each sourdough, the ITS1 metabarcoding analysis identified an increased number of fungal species in sourdough communities. Two third of the identified sequences obtained from sourdoughs were Saccharomycetales, mostly in the Kazachstania genus. No Saccharomycetales species was shared by all the sourdoughs, whereas five other fungal species, mainly known plant pathogens, were found in all sourdoughs. Interestingly, Saccharomyces cerevisiae, known as \"baker's yeast,\" was identified as the dominant species in only one sourdough. By contrast, five Kazachstania species were identified as the dominant sourdough species, including one recently described Kazachstania species, Kazachstania saulgeensis and an undescribed Kazachstania sp. Sourdoughs from farmer-bakers harbored Kazachstania bulderi, Kazachstania unispora and two newly described Kazachstania species, while sourdough from bakers mostly carried Kazachstania humilis as the dominant species. Such yeast diversity has not been found in sourdoughs before, highlighting the need to maintain different traditional food practices to conserve microbial diversity.", "gemini": "Các quần xã vi sinh vật rất cần thiết cho việc duy trì và hoạt động của các hệ sinh thái, bao gồm cả hệ sinh thái thực phẩm lên men. Phân tích các quần xã vi sinh vật trong thực phẩm chủ yếu tập trung vào vi khuẩn axit lactic (LAB), trong khi sự đa dạng của nấm men vẫn chưa được hiểu rõ. Ở đây, chúng tôi mô tả sự đa dạng của nấm trong một sản phẩm thực phẩm lên men điển hình, bánh mì chua. Đa dạng loài của 14 loại bánh mì chua thu thập được từ các tiệm bánh trên khắp nước Pháp đã được phân tích. Các tiệm bánh được chọn để đại diện cho các phương pháp làm bánh đa dạng và bao gồm cả thợ làm bánh và nông dân làm bánh. Cả hai phương pháp không dựa trên nuôi cấy (giải trình tự pyro của các đoạn nối phiên mã nội bộ 1) và dựa trên nuôi cấy đều được sử dụng. Trong khi cả hai phương pháp nhận dạng đều thống nhất về loài nấm men chiếm ưu thế của từng loại bánh mì chua, thì phân tích giải trình tự mã vạch ITS1 đã xác định được số lượng loài nấm tăng lên trong các quần xã bánh mì chua. Hai phần ba các trình tự được xác định thu được từ bánh mì chua là Saccharomycetales, chủ yếu thuộc chi Kazachstania. Không có loài Saccharomycetales nào được chia sẻ bởi tất cả các loại bánh mì chua, trong khi năm loài nấm khác, chủ yếu là các tác nhân gây bệnh thực vật đã biết, được tìm thấy trong tất cả các loại bánh mì chua. Điều thú vị là Saccharomyces cerevisiae, được gọi là \"nấm men làm bánh\", chỉ được xác định là loài chiếm ưu thế trong một loại bánh mì chua. Ngược lại, năm loài Kazachstania được xác định là loài bánh mì chua chiếm ưu thế, bao gồm một loài Kazachstania mới được mô tả gần đây, Kazachstania saulgeensis và một loài Kazachstania sp chưa được mô tả. Bánh mì chua từ những người nông dân làm bánh có chứa Kazachstania bulderi, Kazachstania unispora và hai loài Kazachstania mới được mô tả, trong khi bánh mì chua từ thợ làm bánh chủ yếu mang Kazachstania humilis là loài chiếm ưu thế. Sự đa dạng của nấm men như vậy chưa từng được tìm thấy trong bánh mì chua trước đây, làm nổi bật nhu cầu duy trì các phương pháp chế biến thực phẩm truyền thống khác nhau để bảo tồn sự đa dạng của vi sinh vật."} +{"idx": 1910034, "en": "Clostridioides difficile is an intestinal human pathogen that uses the opportunity of a depleted microbiota to cause an infection. It is known, that the composition of the intestinal bile acid cocktail has a great impact on the susceptibility toward a C. difficile infection. However, the specific response of growing C. difficile cells to diverse bile acids on the molecular level has not been described yet. In this study, we recorded proteome signatures of shock and long-term (LT) stress with the four main bile acids cholic acid (CA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), and lithocholic acid (LCA). A general overlapping response to all tested bile acids could be determined particularly in shock experiments which appears plausible in the light of their common steroid structure. However, during LT stress several proteins showed an altered abundance in the presence of only a single or a few of the bile acids indicating the existence of specific adaptation mechanisms. Our results point at a differential induction of the groEL and dnaKJgrpE chaperone systems, both belonging to the class I heat shock genes. Additionally, central metabolic pathways involving butyrate fermentation and the reductive Stickland fermentation of leucine were effected, although CA caused a proteome signature different from the other three bile acids. Furthermore, quantitative proteomics revealed a loss of flagellar proteins in LT stress with LCA. The absence of flagella could be substantiated by electron microscopy which also indicated less flagellated cells in the presence of DCA and CDCA and no influence on flagella formation by CA. Our data break down the bile acid stress response of C. difficile into a general and a specific adaptation. The latter cannot simply be divided into a response to primary and secondary bile acids, but rather reflects a complex and variable adaptation process enabling C. difficile to survive and to cause an infection in the intestinal tract.", "gemini": "Clostridioides difficile là một tác nhân gây bệnh đường ruột ở người sử dụng cơ hội của hệ vi sinh vật bị suy yếu để gây nhiễm trùng. Người ta biết rằng thành phần của hỗn hợp axit mật đường ruột có tác động rất lớn đến khả năng mắc bệnh nhiễm C. difficile. Tuy nhiên, phản ứng cụ thể của các tế bào C. difficile đang phát triển đối với các axit mật đa dạng ở cấp độ phân tử vẫn chưa được mô tả. Trong nghiên cứu này, chúng tôi đã ghi lại các đặc điểm của proteome về sốc và căng thẳng dài hạn (LT) với bốn axit mật chính là axit cholic (CA), axit chenodeoxycholic (CDCA), axit deoxycholic (DCA) và axit lithocholic (LCA). Một phản ứng chồng chéo chung đối với tất cả các axit mật được thử nghiệm có thể được xác định đặc biệt trong các thí nghiệm sốc, điều này có vẻ hợp lý khi xét đến cấu trúc steroid chung của chúng. Tuy nhiên, trong quá trình căng thẳng LT, một số protein đã biểu hiện sự thay đổi về số lượng khi chỉ có một hoặc một vài axit mật có mặt, chỉ ra sự tồn tại của các cơ chế thích nghi cụ thể. Kết quả của chúng tôi chỉ ra sự cảm ứng khác biệt của các hệ chaperone groEL và dnaKJgrpE, cả hai đều thuộc về gen sốc nhiệt loại I. Ngoài ra, các con đường trao đổi chất trung tâm liên quan đến quá trình lên men butyrat và quá trình lên men Stickland khử leucine đã bị ảnh hưởng, mặc dù CA gây ra đặc điểm proteome khác với ba axit mật còn lại. Hơn nữa, proteomics định lượng cho thấy sự mất các protein roi trong quá trình căng thẳng LT với LCA. Sự vắng mặt của roi có thể được chứng minh bằng kính hiển vi điện tử, kính hiển vi này cũng chỉ ra ít tế bào có roi hơn khi có mặt DCA và CDCA và không ảnh hưởng đến sự hình thành roi bởi CA. Dữ liệu của chúng tôi phân tích phản ứng căng thẳng axit mật của C. difficile thành sự thích nghi chung và sự thích nghi cụ thể. Sự thích nghi sau không thể chỉ đơn giản được chia thành phản ứng với axit mật chính và axit mật thứ cấp, mà phản ánh một quá trình thích nghi phức tạp và thay đổi cho phép C. difficile tồn tại và gây nhiễm trùng ở đường ruột."} +{"idx": 1910035, "en": "We aimed to analyze the molecular characteristics, clonality and antimicrobial resistance profiles of group B streptococcus (GBS) isolates collected in Taiwan from invasive diseases and carriage. Multilocus sequence typing (MLST) was used to assess the genetic diversity of 225 GBS strains from neonates and adults with invasive GBS diseases. 100 GBS strains collected from colonized pregnant women during the same period were compared, and all strains were characterized for one of nine capsule genotypes. We also determined the susceptibilities of all GBS isolates to various antimicrobial agents. The most frequently identified serotypes that caused invasive disease in neonates were III (60.6%) and Ia (17.3%), whereas type VI (32.7%), Ib (19.4%), and V (19.4%) were the most common to cause invasive disease in adults. Serotype VI was the leading type that colonized pregnant women (35.0%). Twenty-six sequence types (STs) were identified, and 90.5% of GBS strains were represented by 6 STs. ST-17 and ST-1 were more prevalent in invasive diseases in neonates and adults, respectively. The majority of serotype III and VI isolates belonged to clonal complex (CC)-17 and CC-1, respectively. ST-17 strains were more likely to cause meningitis and late-onset disease than other strains. In addition, ST-12 and ST-17 GBS strains showed the highest rate of resistance to erythromycin and clindamycin (range: 75.8-100%). In conclusion, CC-17/type III and CC-1/type VI are the most important invasive pathogens in infants and non-pregnant adults in Taiwan, respectively. GBS genotypes vary between different age groups and geographical areas and should be considered during GBS vaccine development.", "gemini": "Chúng tôi nhằm mục đích phân tích các đặc điểm phân tử, tính vô tính và hồ sơ kháng thuốc của các phân lập liên cầu khuẩn nhóm B (GBS) được thu thập tại Đài Loan từ các bệnh xâm lấn và tình trạng mang mầm bệnh. Phân loại trình tự đa gen (MLST) được sử dụng để đánh giá sự đa dạng di truyền của 225 chủng GBS từ trẻ sơ sinh và người lớn mắc bệnh GBS xâm lấn. 100 chủng GBS được thu thập từ phụ nữ mang thai bị nhiễm trùng trong cùng thời kỳ đã được so sánh và tất cả các chủng đều được đặc trưng cho một trong chín kiểu gen vỏ. Chúng tôi cũng xác định độ nhạy cảm của tất cả các phân lập GBS đối với nhiều tác nhân chống vi khuẩn khác nhau. Các huyết thanh phổ biến nhất gây ra bệnh xâm lấn ở trẻ sơ sinh là III (60,6%) và Ia (17,3%), trong khi loại VI (32,7%), Ib (19,4%) và V (19,4%) là phổ biến nhất gây ra bệnh xâm lấn ở người lớn. Huyết thanh VI là loại hàng đầu gây nhiễm trùng ở phụ nữ mang thai (35,0%). Hai mươi sáu loại trình tự (ST) đã được xác định và 90,5% các chủng GBS được biểu thị bằng 6 ST. ST-17 và ST-1 phổ biến hơn ở các bệnh xâm lấn ở trẻ sơ sinh và người lớn. Phần lớn các phân lập huyết thanh III và VI thuộc về phức hợp vô tính (CC) -17 và CC-1. Các chủng ST-17 có khả năng gây viêm màng não và bệnh khởi phát muộn cao hơn các chủng khác. Ngoài ra, các chủng GBS ST-12 và ST-17 cho thấy tỷ lệ kháng erythromycin và clindamycin cao nhất (phạm vi: 75,8-100%). Tóm lại, CC-17 / loại III và CC-1 / loại VI lần lượt là các tác nhân gây bệnh xâm lấn quan trọng nhất ở trẻ sơ sinh và người lớn không mang thai ở Đài Loan. Các kiểu gen GBS khác nhau giữa các nhóm tuổi và khu vực địa lý khác nhau và nên được xem xét trong quá trình phát triển vắc-xin GBS."} +{"idx": 1910036, "en": "Koplik spots are considered a disease-specific sign for measles, although comprehensive virological studies have not been conducted to date. In Japan, a national survey of 3023 measles and measles-suspected cases was conducted between 2009 and 2014 using polymerase chain reaction (PCR) or reverse transcription PCR (RT-PCR) to detect various rash/fever-associated viruses. Koplik spots were observed in 717 of 3023 cases (23.7%). Among these, the measles virus was detected in 202 cases (28.2%), while the rubella virus was detected in 125 cases (17.4%). Other viruses were detected in 51 cases having the spots (7.1%). In some of the cases with spots, two or three viruses, such as the rubella virus, parvovirus, and human herpesvirus type 6 were also detected. The sensitivity and specificity of Koplik spots as a diagnostic marker for measles were 48 and 80%, respectively. The results suggested that Koplik spots might appear not only in measles but also in other viral infections, such as rubella, as a clinical sign.", "gemini": "Các đốm Koplik được coi là dấu hiệu đặc hiệu của bệnh sởi, mặc dù cho đến nay vẫn chưa có các nghiên cứu toàn diện về virus học được tiến hành. Tại Nhật Bản, một cuộc khảo sát quốc gia về 3023 trường hợp mắc sởi và nghi ngờ mắc sởi đã được tiến hành trong giai đoạn 2009 đến 2014 bằng cách sử dụng phản ứng chuỗi polymerase (PCR) hoặc phản ứng chuỗi phiên mã ngược (RT-PCR) để phát hiện nhiều loại virus liên quan đến phát ban/sốt. Các đốm Koplik được quan sát thấy ở 717 trong số 3023 trường hợp (23,7%). Trong số các trường hợp này, virus sởi được phát hiện ở 202 trường hợp (28,2%), trong khi virus rubella được phát hiện ở 125 trường hợp (17,4%). Các loại virus khác được phát hiện ở 51 trường hợp có các đốm (7,1%). Trong một số trường hợp có các đốm, hai hoặc ba loại virus, chẳng hạn như virus rubella, parvovirus và virus herpes ở người type 6 cũng được phát hiện. Độ nhạy và độ đặc hiệu của các đốm Koplik như một dấu hiệu chẩn đoán bệnh sởi lần lượt là 48 và 80%. Kết quả cho thấy các đốm Koplik có thể xuất hiện không chỉ ở bệnh sởi mà còn ở các bệnh nhiễm virus khác, chẳng hạn như rubella, như một dấu hiệu lâm sàng."} +{"idx": 1910037, "en": "Mast cells (MC) are important immune sentinels found in most tissue and widely recognized for their role as mediators of Type I hypersensitivity. However, they also secrete anti-cancer mediators such as tumor necrosis factor alpha (TNF-α) and granulocyte-macrophage colony-stimulating factor (GM-CSF). The purpose of this study was to investigate adipose tissue as a new source of MC in quantities that could be used to study MC biology focusing on their ability to bind to and kill breast cancer cells. We tested several cell culture media previously demonstrated to induce MC differentiation. We report here the generation of functional human MC from adipose tissue. The adipose-derived mast cells (ADMC) are phenotypically and functionally similar to connective tissue expressing tryptase, chymase, c-kit, and FcεRI and capable of degranulating after cross-linking of FcεRI. The ADMC, sensitized with anti-HER2/neu IgE antibodies with human constant regions (trastuzumab IgE and/or C6MH3-B1 IgE), bound to and released MC mediators when incubated with HER2/neu-positive human breast cancer cells (SK-BR-3 and BT-474). Importantly, the HER2/neu IgE-sensitized ADMC induced breast cancer cell (SK-BR-3) death through apoptosis. Breast cancer cell apoptosis was observed after the addition of cell-free supernatants containing mediators released from FcεRI-challenged ADMC. Apoptosis was significantly reduced when TNF-α blocking antibodies were added to the media. Adipose tissue represents a source MC that could be used for multiple research purposes and potentially as a cell-mediated cancer immunotherapy through the expansion of autologous (or allogeneic) MC that can be targeted to tumors through IgE antibodies recognizing tumor specific antigens.", "gemini": "Tế bào mast (MC) là những vệ sĩ miễn dịch quan trọng được tìm thấy trong hầu hết các mô và được công nhận rộng rãi về vai trò của chúng như chất trung gian của phản ứng quá mẫn loại I. Tuy nhiên, chúng cũng tiết ra các chất trung gian chống ung thư như yếu tố hoại tử khối u alpha (TNF-α) và yếu tố kích thích tập đoàn đại thực bào hạt (GM-CSF). Mục đích của nghiên cứu này là để điều tra mô mỡ như một nguồn MC mới với số lượng có thể được sử dụng để nghiên cứu sinh học MC tập trung vào khả năng liên kết và tiêu diệt các tế bào ung thư vú của chúng. Chúng tôi đã thử nghiệm một số môi trường nuôi cấy tế bào trước đây đã được chứng minh là có thể gây ra sự biệt hóa của MC. Chúng tôi báo cáo ở đây về việc tạo ra MC chức năng của con người từ mô mỡ. Các tế bào mast có nguồn gốc từ mô mỡ (ADMC) có kiểu hình và chức năng tương tự như mô liên kết biểu hiện tryptase, chymase, c-kit và FcεRI và có khả năng giải phóng hạt sau khi liên kết chéo FcεRI. ADMC, được nhạy cảm với các kháng thể IgE kháng HER2/neu có vùng hằng số của người (trastuzumab IgE và/hoặc C6MH3-B1 IgE), liên kết với và giải phóng các chất trung gian MC khi được ủ với các tế bào ung thư vú của người dương tính với HER2/neu (SK-BR-3 và BT-474). Quan trọng hơn, ADMC nhạy cảm với IgE HER2/neu gây ra cái chết của tế bào ung thư vú (SK-BR-3) thông qua quá trình apoptosis. Quá trình apoptosis của tế bào ung thư vú đã được quan sát thấy sau khi bổ sung các dịch nuôi cấy không có tế bào chứa các chất trung gian được giải phóng từ ADMC bị FcεRI tấn công. Quá trình apoptosis giảm đáng kể khi các kháng thể chặn TNF-α được thêm vào môi trường. Mô mỡ đại diện cho một nguồn MC có thể được sử dụng cho nhiều mục đích nghiên cứu và có khả năng là một liệu pháp miễn dịch ung thư qua trung gian tế bào thông qua việc mở rộng MC tự thân (hoặc đồng loại) có thể nhắm mục tiêu vào các khối u thông qua các kháng thể IgE nhận dạng các kháng nguyên đặc hiệu của khối u."} +{"idx": 1910038, "en": "Antigen presentation on HLA molecules is a major mechanism by which the immune system monitors self and non-self-recognition. Importantly, HLA-I presentation has gained much attention through its role in eliciting anti-tumor immunity. Several determinants controlling the peptides presented on HLA have been uncovered, mainly through the study of model substrates and large-scale immunopeptidome analyses. These determinants include the relative abundances of proteins in the cell, the stability or turnover rate of these proteins and the binding affinities of a given peptide to the HLA haplotypes found in a cell. However, the regulatory principles involved in selection and regulation of specific antigens in response to tumor pro-inflammatory signals remain largely unknown. Here, we chose to examine the effect that TNFα and IFNγ stimulation may exert on the immunopeptidome landscape of lung cancer cells. We show that the expression of many of the proteins involved in the class I antigen presentation pathway are changed by pro-inflammatory cytokines. Further, we could show that increased expression of the HLA-B allomorph drives a significant change in HLA-bound antigens, independently of the significant changes observed in the cellular proteome. Finally, we observed increased HLA-B levels in correlation with tumor infiltration across the TCGA lung cancer cohorts. Taken together, our results suggest that the immunopeptidome landscape should be examined in the context of anti-tumor immunity whereby signals in the microenvironment may be critical in shaping and modulating this important aspect of host-tumor interactions.", "gemini": "Trình bày kháng nguyên trên các phân tử HLA là một cơ chế chính mà hệ thống miễn dịch theo dõi sự nhận biết bản thân và không phải bản thân. Quan trọng hơn, trình bày HLA-I đã nhận được nhiều sự chú ý thông qua vai trò của nó trong việc kích thích miễn dịch chống khối u. Một số yếu tố quyết định kiểm soát các peptide được trình bày trên HLA đã được phát hiện, chủ yếu thông qua nghiên cứu về các chất nền mô hình và phân tích miễn dịch peptide quy mô lớn. Các yếu tố quyết định này bao gồm sự phong phú tương đối của protein trong tế bào, tốc độ ổn định hoặc luân chuyển của các protein này và ái lực liên kết của một peptide nhất định với các kiểu gen HLA có trong tế bào. Tuy nhiên, các nguyên tắc điều chỉnh liên quan đến quá trình lựa chọn và điều chỉnh các kháng nguyên cụ thể để đáp ứng với các tín hiệu gây viêm của khối u vẫn chưa được biết đến nhiều. Ở đây, chúng tôi chọn kiểm tra tác dụng mà sự kích thích TNFα và IFNγ có thể gây ra đối với cảnh quan miễn dịch peptide của các tế bào ung thư phổi. Chúng tôi chỉ ra rằng biểu hiện của nhiều protein tham gia vào con đường trình bày kháng nguyên lớp I bị thay đổi bởi các cytokine gây viêm. Hơn nữa, chúng tôi có thể chỉ ra rằng sự gia tăng biểu hiện của allomorph HLA-B thúc đẩy một sự thay đổi đáng kể trong các kháng nguyên liên kết với HLA, độc lập với những thay đổi đáng kể được quan sát thấy trong proteome của tế bào. Cuối cùng, chúng tôi quan sát thấy mức HLA-B tăng lên có tương quan với sự xâm nhập của khối u trên các nhóm ung thư phổi TCGA. Kết hợp lại, kết quả của chúng tôi cho thấy rằng cảnh quan miễn dịch peptide nên được kiểm tra trong bối cảnh miễn dịch chống khối u, trong đó các tín hiệu trong vi môi trường có thể rất quan trọng trong việc định hình và điều chỉnh khía cạnh quan trọng này của tương tác giữa vật chủ và khối u."} +{"idx": 1910039, "en": "Rice leaffolder, Cnaphalocrocis medinalis is one of the key foliage feeding insects of great concern throughout Asia as it results in significant yield losses. High visibility of damage is triggering farmers to apply toxic pesticides for its management. Therefore, it is vital to identify new stable sources of resistance for leaffolder. Phenotyping of 160 recombinant inbred lines (RILs) of a cross between a resistant parent, W1263 and a susceptible parent, TN1 using a rapid field screening method for three seasons resulted in identification of nine RILs as stable sources of resistance to rice leaffolder. Phenotypic frequency distributions were found continuous indicating that the resistance is a quantitative trait governed by polygenes. Phenotypic data for three seasons were analyzed using Genotype and Genotype × Environment Interaction (GGE) analysis for identification of stable resistant lines. Additive main effect and multiplicative interaction (AMMI) analysis showed that 86.41% of the total sum of square of damaged leaf area was attributed to genotype (GEN) effect; 0.48% to environment (ENV) effects and 5.68% to genotype by environment (G × E) interaction effects. Damage area, damage score and leaf length showed very high broad-sense heritability across three environments. However, leaf width had low heritability indicating higher environment influence. Phylogenetic analysis grouped these 160 RILs and parents into five clusters based on resistant reaction. AMMI and GGE biplot analysis revealed that stable genotypes G8 (MP114) and G3 (MP108) with lower damage area and damage score can be utilized in developing cultivars with leaffolder resistance.", "gemini": "Sâu cuốn lá lúa, Cnaphalocrocis medinalis là một trong những loài côn trùng gây hại chính trên lá gây thiệt hại đáng kể ở khắp châu Á do làm giảm năng suất đáng kể. Tình trạng thiệt hại dễ thấy khiến nông dân phải sử dụng thuốc trừ sâu độc hại để quản lý. Do đó, việc xác định các nguồn kháng mới ổn định đối với sâu cuốn lá là rất quan trọng. Phân tích kiểu hình của 160 dòng cận trùng hợp tái tổ hợp (RIL) của phép lai giữa bố mẹ kháng, W1263 và bố mẹ dễ nhiễm, TN1 bằng phương pháp sàng lọc nhanh ngoài đồng trong ba mùa đã xác định được chín RIL là nguồn kháng ổn định đối với sâu cuốn lá lúa. Phân bố tần suất kiểu hình được tìm thấy liên tục chỉ ra rằng khả năng kháng là một tính trạng định lượng do nhiều gen chi phối. Dữ liệu kiểu hình trong ba mùa được phân tích bằng phân tích Tương tác kiểu gen và kiểu gen × Môi trường (GGE) để xác định các dòng kháng ổn định. Phân tích hiệu ứng chính cộng và tương tác nhân (AMMI) cho thấy 86,41% tổng bình phương tổn thất diện tích lá bị hại là do hiệu ứng kiểu gen (GEN); 0,48% do hiệu ứng môi trường (ENV) và 5,68% do hiệu ứng tương tác kiểu gen x môi trường (G × E). Diện tích bị hại, điểm bị hại và chiều dài lá cho thấy khả năng di truyền theo nghĩa rộng rất cao trên ba môi trường. Tuy nhiên, chiều rộng lá có khả năng di truyền thấp, chỉ ra ảnh hưởng của môi trường cao hơn. Phân tích phát sinh loài đã nhóm 160 RIL và bố mẹ này thành năm cụm dựa trên phản ứng kháng. Phân tích đồ thị hai chiều AMMI và GGE cho thấy các kiểu gen ổn định G8 (MP114) và G3 (MP108) có diện tích bị hại và điểm bị hại thấp hơn có thể được sử dụng để phát triển các giống có khả năng kháng sâu cuốn lá."} +{"idx": 1910040, "en": "In our previous study, several iron-related outer membrane proteins in Aeromonas hydrophila, a serious pathogen of farmed fish, conferred high immunoprotectivity to fish, and were proposed as potential vaccine candidates. However, the protective efficacy of these extracellular proteins against A. hydrophila remains largely unknown. Here, we identified secreted proteins that were differentially expressed in A. hydrophila LP-2 in response to iron starvation using an iTRAQ-based quantitative proteomics method. We identified 341 proteins, of which 9 were upregulated in response to iron starvation and 24 were downregulated. Many of the differently expressed proteins were associated with protease activity. We confirmed our proteomics results with Western blotting and qPCR. We constructed three mutants by knocking out three genes encoding differentially expressed proteins (Δorf01830, Δorf01609, and Δorf03641). The physiological characteristics of these mutants were investigated. In all these mutant strains, protease activity decreased, and Δorf01609, and Δorf01830 were less virulent in zebrafish. This indicated that the proteins encoded by these genes may play important roles in bacterial infection. We next evaluated the immune response provoked by the six iron-related recombinant proteins (ORF01609, ORF01830, ORF01839, ORF02943, ORF03355, and ORF03641) in zebrafish as well as the immunization efficacy of these proteins. Immunization with these proteins significantly increased the zebrafish immune response. In addition, the relative percent survival (RPS) of the immunized zebrafish was 50-80% when challenged with three virulent A. hydrophila strains, respectively. Thus, these extracellular secreted proteins might be effective vaccine candidates against A. hydrophila infection in fish.", "gemini": "Trong nghiên cứu trước đây của chúng tôi, một số protein màng ngoài liên quan đến sắt trong Aeromonas hydrophila, một tác nhân gây bệnh nghiêm trọng ở cá nuôi, đã mang lại khả năng bảo vệ miễn dịch cao cho cá và được đề xuất là ứng cử viên vắc-xin tiềm năng. Tuy nhiên, hiệu quả bảo vệ của các protein ngoại bào này chống lại A. hydrophila vẫn chưa được biết đến nhiều. Ở đây, chúng tôi xác định các protein tiết ra được biểu hiện khác biệt trong A. hydrophila LP-2 để đáp ứng với tình trạng thiếu sắt bằng phương pháp định lượng proteomics dựa trên iTRAQ. Chúng tôi đã xác định được 341 protein, trong đó 9 protein được điều hòa tăng lên để đáp ứng với tình trạng thiếu sắt và 24 protein được điều hòa giảm. Nhiều protein biểu hiện khác nhau có liên quan đến hoạt động của protease. Chúng tôi đã xác nhận kết quả proteomics của mình bằng phương pháp Western blotting và qPCR. Chúng tôi đã xây dựng ba đột biến bằng cách loại bỏ ba gen mã hóa các protein biểu hiện khác nhau (Δorf01830, Δorf01609 và Δorf03641). Các đặc điểm sinh lý của các đột biến này đã được nghiên cứu. Ở tất cả các chủng đột biến này, hoạt động của protease giảm và Δorf01609 và Δorf01830 ít độc lực hơn ở cá ngựa vằn. Điều này chỉ ra rằng các protein được mã hóa bởi các gen này có thể đóng vai trò quan trọng trong nhiễm trùng do vi khuẩn. Tiếp theo, chúng tôi đánh giá phản ứng miễn dịch do sáu protein tái tổ hợp liên quan đến sắt (ORF01609, ORF01830, ORF01839, ORF02943, ORF03355 và ORF03641) ở cá ngựa vằn cũng như hiệu quả miễn dịch của các protein này. Miễn dịch với các protein này làm tăng đáng kể phản ứng miễn dịch của cá ngựa vằn. Ngoài ra, tỷ lệ phần trăm sống tương đối (RPS) của cá ngựa vằn được miễn dịch là 50-80% khi lần lượt thử nghiệm với ba chủng A. hydrophila độc lực. Do đó, các protein tiết ngoài tế bào này có thể là ứng cử viên vắc-xin hiệu quả chống lại nhiễm trùng A. hydrophila ở cá."} +{"idx": 1910041, "en": "Phospholipase D alpha 1 (PLDα1) is a phospholipid hydrolyzing enzyme playing multiple regulatory roles in stress responses of plants. Its signaling activity is mediated by phosphatidic acid (PA) production, capacity to bind, and modulate G-protein complexes or by interaction with other proteins. This work presents a quantitative proteomic analysis of two T-DNA insertion pldα1 mutants of Arabidopsis thaliana. Remarkably, PLDα1 knockouts caused differential regulation of many proteins forming protein complexes, while PLDα1 might be required for their stability. Almost one third of differentially abundant proteins (DAPs) in pldα1 mutants are implicated in metabolism and RNA binding. Latter functional class comprises proteins involved in translation, RNA editing, processing, stability, and decay. Many of these proteins, including those regulating chloroplast protein import and protein folding, share common functions in chloroplast biogenesis and leaf variegation. Consistently, pldα1 mutants showed altered level of TIC40 (a major regulator of protein import into chloroplast), differential accumulation of photosynthetic protein complexes and changed chloroplast sizes as revealed by immunoblotting, blue-native electrophoresis, and microscopic analyses, respectively. Our proteomic analysis also revealed that genetic depletion of PLDα1 also affected proteins involved in cell wall architecture, redox homeostasis, and abscisic acid signaling. Taking together, PLDα1 appears as a protein integrating cytosolic and plastidic protein translations, plastid protein degradation, and protein import into chloroplast in order to regulate chloroplast biogenesis in Arabidopsis.", "gemini": "Phospholipase D alpha 1 (PLDα1) là một enzyme thủy phân phospholipid đóng nhiều vai trò điều hòa trong phản ứng căng thẳng của thực vật. Hoạt động truyền tín hiệu của nó được trung gian bởi quá trình sản xuất axit phosphatidic (PA), khả năng liên kết và điều chỉnh các phức hợp protein G hoặc bằng cách tương tác với các protein khác. Công trình này trình bày phân tích định lượng proteomic của hai đột biến pldα1 chèn T-DNA của Arabidopsis thaliana. Đáng chú ý, các gen loại trừ PLDα1 gây ra sự điều hòa khác biệt của nhiều protein tạo thành các phức hợp protein, trong khi PLDα1 có thể là cần thiết cho sự ổn định của chúng. Gần một phần ba các protein có nhiều khác biệt (DAP) trong các đột biến pldα1 có liên quan đến quá trình trao đổi chất và liên kết RNA. Lớp chức năng sau bao gồm các protein tham gia vào quá trình dịch mã, chỉnh sửa RNA, xử lý, ổn định và phân hủy. Nhiều protein trong số này, bao gồm cả những protein điều hòa quá trình nhập khẩu protein lục lạp và gấp protein, có chức năng chung trong quá trình sinh tổng hợp lục lạp và sự thay đổi màu của lá. Phù hợp với điều đó, các đột biến pldα1 cho thấy mức độ thay đổi của TIC40 (một chất điều hòa chính của quá trình nhập protein vào lục lạp), sự tích lũy khác biệt của các phức hợp protein quang hợp và thay đổi kích thước lục lạp như được tiết lộ lần lượt bằng phương pháp miễn dịch, điện di blue-native và phân tích kính hiển vi. Phân tích proteomic của chúng tôi cũng cho thấy sự suy giảm di truyền của PLDα1 cũng ảnh hưởng đến các protein liên quan đến cấu trúc thành tế bào, cân bằng oxy hóa khử và truyền tín hiệu axit abscisic. Tổng hợp lại, PLDα1 xuất hiện như một protein tích hợp quá trình dịch mã protein ở tế bào chất và lục lạp, quá trình phân hủy protein ở lục lạp và quá trình nhập protein vào lục lạp để điều hòa quá trình sinh tổng hợp lục lạp ở Arabidopsis."} +{"idx": 1910042, "en": "For large scale production of clonal plants, somatic embryogenesis (SE) has many advantages over other clonal propagation methods such as the rooting of cuttings. In particular, the SE process is more suited to scale up and automation, thereby reducing labor costs and increasing the reliability of the production process. Furthermore, the plants resulting from SE closely resemble those from seeds, as somatic embryos, like zygotic (seed) embryos, develop with good connection between root and shoot, and without the plagiotropism often associated with propagation by cuttings. For practical purposes in breeding programs and for deployment of elite clones, it is valuable that a virtually unlimited number of SE plants can be generated from one original seed embryo; and SE cultures (clones) can be cryostored for at least 20 years, allowing long-term testing of clones. To date, there has however been limited use of SE for large-scale plant production mainly because without automation it is labor-intensive. Development of automation is particularly attractive in countries with high labor costs, where conifer forestry is often of great economic importance. Various approaches for automating SE processes are under investigation and the progress is reviewed here, with emphasis on conifers. These approaches include simplification of culture routines with preference for liquid rather than solid cultures, use of robotics and automation for the harvest of selected individual mature embryos, followed by automated handling of germination and subsequent planting. Different approaches to handle the processes of somatic embryogenesis in conifers are outlined below, followed by an update on efforts to automate the different steps, which are nearing an operational stage.", "gemini": "Đối với sản xuất cây vô tính quy mô lớn, phôi phôi soma (SE) có nhiều ưu điểm hơn so với các phương pháp nhân giống vô tính khác như ra rễ của cành giâm. Đặc biệt, quá trình SE phù hợp hơn để mở rộng quy mô và tự động hóa, do đó giảm chi phí lao động và tăng độ tin cậy của quá trình sản xuất. Hơn nữa, các cây thu được từ SE rất giống với cây từ hạt, vì phôi phôi soma, giống như phôi hợp tử (hạt), phát triển với sự kết nối tốt giữa rễ và chồi, và không có hiện tượng hướng quang âm thường liên quan đến việc nhân giống bằng cành giâm. Đối với các mục đích thực tế trong các chương trình lai tạo và để triển khai các dòng vô tính ưu tú, có giá trị là có thể tạo ra số lượng cây SE gần như không giới hạn từ một phôi hạt ban đầu; và các nuôi cấy SE (vô tính) có thể được bảo quản lạnh trong ít nhất 20 năm, cho phép thử nghiệm các dòng vô tính trong thời gian dài. Tuy nhiên, cho đến nay, việc sử dụng SE để sản xuất cây quy mô lớn còn hạn chế chủ yếu là do không có tự động hóa, nên cần nhiều nhân công. Việc phát triển tự động hóa đặc biệt hấp dẫn ở các quốc gia có chi phí lao động cao, nơi lâm nghiệp lá kim thường có tầm quan trọng kinh tế lớn. Nhiều cách tiếp cận khác nhau để tự động hóa các quy trình SE đang được nghiên cứu và tiến trình được xem xét tại đây, với trọng tâm là cây lá kim. Các cách tiếp cận này bao gồm đơn giản hóa quy trình nuôi cấy ưu tiên cho nuôi cấy lỏng hơn là nuôi cấy rắn, sử dụng robot và tự động hóa để thu hoạch các phôi trưởng thành riêng lẻ đã chọn, sau đó là xử lý tự động quá trình nảy mầm và trồng tiếp theo. Các cách tiếp cận khác nhau để xử lý các quy trình phôi phôi soma ở cây lá kim được nêu dưới đây, tiếp theo là cập nhật về các nỗ lực tự động hóa các bước khác nhau, hiện đang tiến gần đến giai đoạn hoạt động."} +{"idx": 1910043, "en": "Worldwide, trees are confronting increased temperature and aridity, exacerbating susceptibility to herbivory. Long-term studies comparing patterns of plant performance through drought can help identify variation among and within populations in vulnerability to climate change and herbivory. We use long-term monitoring data to examine our overarching hypothesis that the negative impacts of poor soil and herbivore susceptibility would be compounded by severe drought. We studied pinyon pine, Pinus edulis, a widespread southwestern tree species that has suffered extensive climate-change related mortality. We analyzed data on mortality, growth, male reproduction, and herbivory collected for 14-32 years in three areas with distinct soil-types. We used standardized precipitation-evapotranspiration index (SPEI) as a climate proxy that summarizes the impacts of drought due to precipitation and temperature variation on semi-arid forests. Several key findings emerged: (1) Plant performance measurements did not support our hypothesis that trees growing in stressful, coarse-textured soils would suffer more than trees growing in finer-textured soils. Stem growth at the area with coarse, young cinder soils (area one) responded only weakly to drought, while stem growth on more developed soils with sedimentary (area two) and volcanic (area three) substrates, was strongly negatively affected by drought. Male reproduction declined less with drought at area one and more at areas two and three. Overall mortality was 30% on coarse cinder soils (area one) and averaged 55% on finer soil types (areas two and three). (2) Although moth herbivore susceptible trees were hypothesized to suffer more with drought than moth resistant trees, the opposite occurred. Annual stem growth was negatively affected by drought for moth resistant trees, but much less strongly for moth susceptible trees. (3) In contrast to our hypothesis, moths declined with drought. Overall, chronically water-stressed and herbivore-susceptible trees had smaller declines in performance relative to less-stressed trees during drought years. These long-term findings support the idea that stressed trees might be more resistant to drought since they may have adapted or acclimated to resist drought-related mortality.", "gemini": "Trên toàn thế giới, cây cối đang phải đối mặt với nhiệt độ tăng và khô cằn, làm trầm trọng thêm tình trạng dễ bị ăn cỏ. Các nghiên cứu dài hạn so sánh các mô hình hoạt động của cây thông qua hạn hán có thể giúp xác định sự khác biệt giữa các quần thể và trong quần thể về mức độ dễ bị tổn thương do biến đổi khí hậu và ăn cỏ. Chúng tôi sử dụng dữ liệu giám sát dài hạn để kiểm tra giả thuyết bao quát của mình rằng những tác động tiêu cực của đất xấu và tình trạng dễ bị ăn cỏ sẽ trở nên trầm trọng hơn do hạn hán nghiêm trọng. Chúng tôi đã nghiên cứu thông pinyon, Pinus edulis, một loài cây phổ biến ở tây nam đã bị chết hàng loạt liên quan đến biến đổi khí hậu. Chúng tôi đã phân tích dữ liệu về tỷ lệ tử vong, tăng trưởng, sinh sản của con đực và tình trạng ăn cỏ được thu thập trong 14-32 năm ở ba khu vực có các loại đất riêng biệt. Chúng tôi đã sử dụng chỉ số bốc hơi-giáng thủy chuẩn hóa (SPEI) làm đại diện cho khí hậu, tóm tắt các tác động của hạn hán do lượng mưa và nhiệt độ thay đổi đối với các khu rừng bán khô cằn. Một số phát hiện chính đã xuất hiện: (1) Các phép đo hiệu suất của cây không ủng hộ giả thuyết của chúng tôi rằng cây mọc trên đất thô, có kết cấu căng sẽ bị ảnh hưởng nhiều hơn cây mọc trên đất có kết cấu mịn hơn. Tăng trưởng thân ở khu vực có đất tro thô, trẻ (khu vực một) chỉ phản ứng yếu với hạn hán, trong khi tăng trưởng thân trên đất phát triển hơn với nền đá trầm tích (khu vực hai) và núi lửa (khu vực ba) bị ảnh hưởng tiêu cực mạnh bởi hạn hán. Sinh sản của con đực giảm ít hơn do hạn hán ở khu vực một và nhiều hơn ở khu vực hai và ba. Tỷ lệ tử vong chung là 30% trên đất tro thô (khu vực một) và trung bình là 55% trên các loại đất mịn hơn (khu vực hai và ba). (2) Mặc dù giả thuyết cho rằng cây dễ bị sâu bướm ăn lá sẽ bị ảnh hưởng nhiều hơn do hạn hán so với cây kháng sâu bướm, nhưng điều ngược lại đã xảy ra. Tăng trưởng thân hàng năm bị ảnh hưởng tiêu cực bởi hạn hán đối với cây kháng sâu bướm, nhưng ít mạnh hơn nhiều đối với cây dễ bị sâu bướm. (3) Trái ngược với giả thuyết của chúng tôi, sâu bướm giảm khi hạn hán. Nhìn chung, cây bị căng nước mãn tính và dễ bị ăn cỏ có mức giảm hiệu suất nhỏ hơn so với cây ít bị căng thẳng trong những năm hạn hán. Những phát hiện dài hạn này ủng hộ ý tưởng rằng cây bị căng thẳng có thể có khả năng chống hạn tốt hơn vì chúng có thể đã thích nghi hoặc thích nghi để chống lại tình trạng tử vong liên quan đến hạn hán."} +{"idx": 1910044, "en": "Climacteric and non-climacteric fruits are differentiated by the ripening process, in particular by the involvement of ethylene, high respiration rates and the nature of the process, being autocatalytic or not, respectively. Here, we focus on the biosynthesis, metabolism and function of three compounds (auxin, salicylic acid and melatonin) sharing not only a common precursor (chorismate), but also regulatory functions in plants, and therefore in fruits. Aside from describing their biosynthesis in plants, with a particular emphasis on common precursors and points of metabolic diversion, we will discuss recent advances on their role in fruit ripening and the regulation of bioactive compounds accumulation, both in climacteric and non-climacteric fruits.", "gemini": "Trái cây thời kỳ mãn kinh và không thời kỳ mãn kinh được phân biệt bởi quá trình chín, đặc biệt là sự tham gia của ethylene, tỷ lệ hô hấp cao và bản chất của quá trình, tương ứng là tự xúc tác hay không. Ở đây, chúng tôi tập trung vào quá trình sinh tổng hợp, chuyển hóa và chức năng của ba hợp chất (auxin, axit salicylic và melatonin) không chỉ có chung một tiền chất (chorismate) mà còn có chức năng điều hòa trong thực vật, và do đó trong trái cây. Ngoài việc mô tả quá trình sinh tổng hợp của chúng trong thực vật, đặc biệt nhấn mạnh đến các tiền chất chung và các điểm chuyển hướng trao đổi chất, chúng tôi sẽ thảo luận về những tiến bộ gần đây về vai trò của chúng trong quá trình chín của trái cây và sự điều chỉnh quá trình tích lũy các hợp chất hoạt tính sinh học, cả trong trái cây thời kỳ mãn kinh và không thời kỳ mãn kinh."} +{"idx": 1910045, "en": "Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are present at low and controlled levels under normal conditions. These reactive molecules can increase to high levels under various biotic and abiotic conditions, resulting in perturbation of the cellular redox state that can ultimately lead to oxidative or nitrosative stress. In this review, we analyze the various effects that result from alterations of redox homeostasis on plant glycolytic pathway and tricarboxylic acid (TCA) cycle. Most documented modifications caused by ROS or RNS are due to the presence of redox-sensitive cysteine thiol groups in proteins. Redox modifications include Cys oxidation, disulfide bond formation, S-glutathionylation, S-nitrosylation, and S-sulfhydration. A growing number of proteomic surveys and biochemical studies document the occurrence of ROS- or RNS-mediated modification in enzymes of glycolysis and the TCA cycle. In a few cases, these modifications have been shown to affect enzyme activity, suggesting an operational regulatory mechanism in vivo. Further changes induced by oxidative stress conditions include the proposed redox-dependent modifications in the subcellular distribution of a putative redox sensor, NAD-glyceraldehyde-3P dehydrogenase and the micro-compartmentation of cytosolic glycolytic enzymes. Data from the literature indicate that oxidative stress may induce complex changes in metabolite pools in central carbon metabolism. This information is discussed in the context of our understanding of plant metabolic response to oxidative stress.", "gemini": "Các loài oxy phản ứng (ROS) và các loài nitơ phản ứng (RNS) có mặt ở mức thấp và được kiểm soát trong điều kiện bình thường. Các phân tử phản ứng này có thể tăng lên mức cao trong các điều kiện sinh học và phi sinh học khác nhau, dẫn đến sự nhiễu loạn trạng thái oxy hóa khử của tế bào, cuối cùng có thể dẫn đến căng thẳng oxy hóa hoặc nitơ hóa. Trong bài đánh giá này, chúng tôi phân tích các hiệu ứng khác nhau phát sinh từ sự thay đổi cân bằng oxy hóa khử trên con đường phân giải đường của thực vật và chu trình axit tricarboxylic (TCA). Hầu hết các sửa đổi được ghi nhận do ROS hoặc RNS gây ra là do sự hiện diện của các nhóm thiol cysteine nhạy với oxy hóa khử trong protein. Các sửa đổi oxy hóa khử bao gồm oxy hóa Cys, hình thành liên kết disulfua, S-glutathionylation, S-nitrosylation và S-sulfhydration. Một số lượng ngày càng tăng các cuộc khảo sát về proteomics và các nghiên cứu sinh hóa ghi lại sự xuất hiện của sửa đổi do ROS hoặc RNS trong các enzym phân giải đường và chu trình TCA. Trong một số ít trường hợp, những sửa đổi này đã được chứng minh là ảnh hưởng đến hoạt động của enzym, cho thấy một cơ chế điều chỉnh hoạt động trong cơ thể sống. Những thay đổi tiếp theo do điều kiện căng thẳng oxy hóa gây ra bao gồm các sửa đổi phụ thuộc vào oxy hóa khử được đề xuất trong phân bố dưới tế bào của một cảm biến oxy hóa khử, NAD-glyceraldehyde-3P dehydrogenase và sự phân chia các enzym phân giải đường trong tế bào chất. Dữ liệu từ các tài liệu cho thấy rằng căng thẳng oxy hóa có thể gây ra những thay đổi phức tạp trong các nhóm chất chuyển hóa trong quá trình chuyển hóa cacbon trung tâm. Thông tin này được thảo luận trong bối cảnh hiểu biết của chúng ta về phản ứng chuyển hóa của thực vật đối với căng thẳng oxy hóa."} +{"idx": 1910046, "en": "MYB transcription factors have been demonstrated to play key regulatory roles in plant growth, development and abiotic stress response. However, knowledge concerning the involvement of rice MYB genes in salinity and drought stress resistance are largely unknown. In the present study, we cloned and characterized the OsMYB6 gene, which was induced by drought and salinity stress. Subcellular localization of OsMYB6-YFP fusion protein in protoplast cells indicated that OsMYB6 was localized in the nucleus. Overexpression of OsMYB6 in rice did not suggest a negative effect on the growth and development of transgenic plants, but OsMYB6-overexpressing plants showed increased tolerance to drought and salt stress compared with wild-type plants, as are evaluated by higher proline content, higher CAT and SOD activities, lower REL and MDA content in transgenic plants under drought and salt stress conditions. In addition, the expression of abiotic stress-responsive genes were significantly higher in OsMYB6 transgenic plants than that in wild-type plants under drought and salt stress conditions. These results indicate that OsMYB6 gene functions as a stress-responsive transcription factor which plays a positive regulatory role in response to drought and salt stress resistance, and may be used as a candidate gene for molecular breeding of salt-tolerant and drought-tolerant crop varieties.", "gemini": "Các yếu tố phiên mã MYB đã được chứng minh là đóng vai trò điều hòa chính trong quá trình tăng trưởng, phát triển của thực vật và phản ứng với căng thẳng phi sinh học. Tuy nhiên, kiến thức liên quan đến sự tham gia của gen MYB lúa trong khả năng chống chịu mặn và hạn hán vẫn chưa được biết đến nhiều. Trong nghiên cứu này, chúng tôi đã nhân bản và đặc trưng gen OsMYB6, gen này được gây ra bởi hạn hán và căng thẳng mặn. Định vị dưới tế bào của protein hòa hợp OsMYB6-YFP trong tế bào nguyên sinh cho thấy OsMYB6 được định vị trong nhân. Việc biểu hiện quá mức OsMYB6 ở lúa không cho thấy tác động tiêu cực đến sự tăng trưởng và phát triển của cây chuyển gen, nhưng cây biểu hiện quá mức OsMYB6 cho thấy khả năng chịu hạn và mặn cao hơn so với cây kiểu hoang dã, được đánh giá bằng hàm lượng proline cao hơn, hoạt động CAT và SOD cao hơn, hàm lượng REL và MDA thấp hơn ở cây chuyển gen trong điều kiện hạn hán và mặn. Ngoài ra, sự biểu hiện của các gen phản ứng với căng thẳng phi sinh học cao hơn đáng kể ở cây chuyển gen OsMYB6 so với cây kiểu hoang dã trong điều kiện hạn hán và mặn. Những kết quả này chỉ ra rằng gen OsMYB6 hoạt động như một yếu tố phiên mã phản ứng với căng thẳng, đóng vai trò điều hòa tích cực trong phản ứng với khả năng chống chịu hạn hán và mặn, và có thể được sử dụng làm gen ứng cử cho lai tạo phân tử các giống cây trồng chịu mặn và chịu hạn."} +{"idx": 1910047, "en": "Unicellular phototrophic algae can form massive blooms with up to millions of individual cells per milliliter in freshwater and marine ecosystems. Despite the temporal dominance of bloom formers many algal species can co-exist and compete for nutrients and space, creating a complex and diverse community. While microscopy and single cell genomics can address the taxonomic inventory, the cellular metabolome has yet to be thoroughly explored to determine the physiological status of microalgae. This might, however, provide a key to understand the observed species diversity in the homogeneous environment. Here, we introduce an effective, rapid and versatile method to analyze living single cells from aqueous substrata with laser-desorption/ionization mass spectrometry (LDI-MS) using a simple and inexpensive matrix-free support. The cells deposited on a cultivation-medium wetted support are analyzed with minimal disturbance as they remain in their natural viable state until their disruption during LDI-MS. Metabolites desorbed from single cells are analyzed on High-Resolution Mass Spectrometry (HR-MS) using the Orbitrap FT-MS technology to fingerprint cellular chemistry. This live single-cell mass spectrometry (LSC-MS) allows assessing the physiological status and strain-specifics of different microalgae, including marine diatoms and freshwater chlorophytes, at the single-cell level. We further report a reliable and robust data treatment pipeline to perform multivariate statistics on the replicated LSC-MS data. Comparing single cell MS spectra from natural phytoplankton samples and from laboratory strains allows the identification and discrimination of inter and intra-specific metabolic variability and thereby has promising applications in addressing highly complex phytoplankton communities. Notably, the herein described matrix-free live-single-cell LDI-HR-MS approach enables monitoring dynamics of the plankton and might explain why key-players survive, thrive, avoid selective feeding or pathogenic virus and bacteria, while others are overcome and die.", "gemini": "Tảo quang dưỡng đơn bào có thể tạo thành các cụm hoa lớn với hàng triệu tế bào riêng lẻ trên một mililít trong hệ sinh thái nước ngọt và biển. Mặc dù sự thống trị tạm thời của các loài tảo tạo hoa nhưng nhiều loài tảo có thể cùng tồn tại và cạnh tranh về chất dinh dưỡng và không gian, tạo ra một cộng đồng phức tạp và đa dạng. Trong khi kính hiển vi và hệ gen tế bào đơn có thể giải quyết được vấn đề kiểm kê phân loại, thì vẫn chưa khám phá kỹ lưỡng về chuyển hóa tế bào để xác định trạng thái sinh lý của tảo siêu nhỏ. Tuy nhiên, điều này có thể cung cấp chìa khóa để hiểu được sự đa dạng về loài được quan sát thấy trong môi trường đồng nhất. Tại đây, chúng tôi giới thiệu một phương pháp hiệu quả, nhanh chóng và linh hoạt để phân tích các tế bào đơn sống từ chất nền dưới nước bằng phương pháp quang phổ khối ion hóa/giải hấp bằng laser (LDI-MS) sử dụng chất hỗ trợ không ma trận đơn giản và rẻ tiền. Các tế bào lắng đọng trên giá đỡ ướt của môi trường nuôi cấy được phân tích với sự nhiễu loạn tối thiểu vì chúng vẫn ở trạng thái sống tự nhiên cho đến khi bị phá vỡ trong quá trình LDI-MS. Các chất chuyển hóa được giải hấp từ các tế bào đơn được phân tích trên phổ khối phân giải cao (HR-MS) sử dụng công nghệ Orbitrap FT-MS để tạo dấu vân tay cho hóa học tế bào. Phổ khối tế bào đơn sống (LSC-MS) này cho phép đánh giá trạng thái sinh lý và đặc điểm chủng loại của các loài tảo siêu nhỏ khác nhau, bao gồm cả tảo cát biển và tảo lục nước ngọt, ở cấp độ tế bào đơn. Hơn nữa, chúng tôi báo cáo một quy trình xử lý dữ liệu đáng tin cậy và mạnh mẽ để thực hiện thống kê đa biến trên dữ liệu LSC-MS được sao chép. So sánh phổ MS tế bào đơn từ các mẫu phù du tự nhiên và từ các chủng trong phòng thí nghiệm cho phép xác định và phân biệt sự biến đổi trao đổi chất giữa các loài và trong loài và do đó có ứng dụng đầy hứa hẹn trong việc giải quyết các cộng đồng phù du cực kỳ phức tạp. Đáng chú ý, phương pháp LDI-HR-MS tế bào đơn sống không ma trận được mô tả ở đây cho phép theo dõi động lực của sinh vật phù du và có thể giải thích tại sao các loài đóng vai trò chính lại tồn tại, phát triển mạnh, tránh được thức ăn chọn lọc hoặc vi khuẩn và vi rút gây bệnh, trong khi những loài khác bị đánh bại và chết."} +{"idx": 1910048, "en": "The circadian clock synchronizes endogenous rhythmic processes with environmental cycles and maximizes plant fitness. Multiple regulatory layers shape circadian oscillation, and chromatin modification is emerging as an important scheme for precise circadian waveforms. Here, we report the role of an evolutionarily conserved Sin3-histone deacetylase complex (HDAC) in circadian oscillation in Arabidopsis. SAP30 FUNCTION-RELATED 1 (AFR1) and AFR2, which are key components of Sin3-HDAC complex, are circadianly-regulated and possibly facilitate the temporal formation of the Arabidopsis Sin3-HDAC complex at dusk. The evening-expressed AFR proteins bind directly to the CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and PSEUDO-RESPONSE REGULATOR 9 (PRR9) promoters and catalyze histone 3 (H3) deacetylation at the cognate regions to repress expression, allowing the declining phase of their expression at dusk. In support, the CCA1 and PRR9 genes were de-repressed around dusk in the afr1-1afr2-1 double mutant. These findings indicate that periodic histone deacetylation at the morning genes by the Sin3-HDAC complex contributes to robust circadian maintenance in higher plants.", "gemini": "Đồng hồ sinh học đồng bộ hóa các quá trình nhịp điệu nội sinh với chu kỳ môi trường và tối đa hóa khả năng thích nghi của thực vật. Nhiều lớp điều hòa khác nhau định hình dao động sinh học và sửa đổi chromatin đang nổi lên như một lược đồ quan trọng cho các dạng sóng sinh học chính xác. Ở đây, chúng tôi báo cáo vai trò của phức hợp Sin3-histone deacetylase (HDAC) được bảo tồn tiến hóa trong dao động sinh học ở Arabidopsis. SAP30 FUNCTION-RELATED 1 (AFR1) và AFR2, là các thành phần chính của phức hợp Sin3-HDAC, được điều hòa theo nhịp sinh học và có thể tạo điều kiện cho sự hình thành tạm thời của phức hợp Sin3-HDAC Arabidopsis vào lúc hoàng hôn. Các protein AFR biểu hiện vào buổi tối liên kết trực tiếp với các vùng khởi động CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) và PSEUDO-RESPONSE REGULATOR 9 (PRR9) và xúc tác giải acetylation histone 3 (H3) ở các vùng nhận biết để ức chế biểu hiện, cho phép giai đoạn biểu hiện của chúng giảm dần vào lúc hoàng hôn. Để hỗ trợ, các gen CCA1 và PRR9 đã được giải ức chế vào khoảng hoàng hôn ở thể đột biến kép afr1-1afr2-1. Những phát hiện này chỉ ra rằng giải acetylation histone định kỳ ở các gen buổi sáng bởi phức hợp Sin3-HDAC góp phần duy trì nhịp sinh học mạnh mẽ ở thực vật bậc cao."} +{"idx": 1910049, "en": "Background: Esophageal squamous cell carcinoma (ESCC) is associated with poor prognosis and occurs with high frequency in China. The germline mutation profile in ESCC remains unclear, and therefore, the discovery of oncogenic alterations in ESCC is urgently needed. This study investigates the germline mutation profile and reveals associations among genotype-environment interactions in ESCC. Methods: Whole exome sequencing and follow-up analysis were performed in 77 matched tumor-normal ESCC specimens to examine the germline profiles. Additionally, associations among genotype-environment interactions were investigated. Results: We identified 84 pathogenic/likely pathogenic mutations and 51 rare variants of uncertain significance (VUS). Twenty VUS with InterVar evidence of a score of moderate pathogenicity (PM) 2/PM2+ supporting pathogenicity (PP) 1 were found to have pathogenic significance. CYP21A2 was the most frequently mutated gene, and the p.Gln319* variant was identified in 6.5% (5/77) of patients. The TP53 p.V197E mutation, located within the DNA binding domain, was found in 1.3% (1/77) of patients. In total, the 11.7% (9/77) of individuals with homologous recombination (HR) VUS were more likely to have well-differentiated tumors than those without (P = 0.003). The degree of lymph node metastasis was correlated with homologous recombination deficiency (HRD) and VUS group (P < 0.05). Moreover, the 10.4% (8/77) of individuals with mismatch repair (MMR) VUS had a higher tumor mutational burden (TMB), although the correlation was not significant. Conclusions: Our study identified the germline mutation profiles in ESCC, providing novel insights into the molecular pathogenesis of this disease. Our results may also serve as a useful resource for the exploration of the underlying mechanism of ESCC and may provide information for the prevention, diagnosis and risk management of ESCC.", "gemini": "Bối cảnh: Ung thư biểu mô tế bào vảy thực quản (ESCC) có liên quan đến tiên lượng xấu và xảy ra với tần suất cao ở Trung Quốc. Hồ sơ đột biến dòng mầm trong ESCC vẫn chưa rõ ràng và do đó, việc phát hiện ra các biến đổi gây ung thư ở ESCC là rất cấp thiết. Nghiên cứu này điều tra hồ sơ đột biến dòng mầm và tiết lộ các mối liên hệ giữa các tương tác kiểu gen-môi trường trong ESCC. Phương pháp: Trình tự toàn bộ exome và phân tích theo dõi đã được thực hiện trên 77 mẫu ESCC khối u-bình thường được ghép nối để kiểm tra hồ sơ dòng mầm. Ngoài ra, các mối liên hệ giữa các tương tác kiểu gen-môi trường đã được điều tra. Kết quả: Chúng tôi đã xác định được 84 đột biến gây bệnh/có khả năng gây bệnh và 51 biến thể hiếm có ý nghĩa không chắc chắn (VUS). Hai mươi VUS có bằng chứng InterVar về điểm gây bệnh vừa phải (PM) 2/PM2+ hỗ trợ tính gây bệnh (PP) 1 được phát hiện có ý nghĩa gây bệnh. CYP21A2 là gen đột biến thường gặp nhất, và biến thể p.Gln319* được xác định ở 6,5% (5/77) bệnh nhân. Đột biến TP53 p.V197E, nằm trong miền liên kết DNA, được tìm thấy ở 1,3% (1/77) bệnh nhân. Tổng cộng, 11,7% (9/77) cá thể có VUS tái tổ hợp tương đồng (HR) có nhiều khả năng có khối u biệt hóa tốt hơn những người không có (P = 0,003). Mức độ di căn hạch bạch huyết có tương quan với tình trạng thiếu hụt tái tổ hợp tương đồng (HRD) và nhóm VUS (P < 0,05). Hơn nữa, 10,4% (8/77) cá thể có VUS sửa chữa không khớp (MMR) có gánh nặng đột biến khối u (TMB) cao hơn, mặc dù mối tương quan không đáng kể. Kết luận: Nghiên cứu của chúng tôi đã xác định hồ sơ đột biến dòng mầm trong ESCC, cung cấp những hiểu biết mới về cơ chế gây bệnh phân tử của căn bệnh này. Kết quả của chúng tôi cũng có thể đóng vai trò là một nguồn tài nguyên hữu ích để khám phá cơ chế tiềm ẩn của ESCC và có thể cung cấp thông tin để phòng ngừa, chẩn đoán và quản lý rủi ro ESCC."} +{"idx": 1910050, "en": "The imputation from lower density SNP chip genotypes to whole-genome sequence level is an established approach to generate high density genotypes for many individuals. Imputation accuracy is dependent on many factors and for small cattle populations such as the endangered German Black Pied cattle (DSN), determining the optimal imputation strategy is especially challenging since only a low number of high density genotypes is available. In this paper, the accuracy of imputation was explored with regard to (1) phasing of the target population and the reference panel for imputation, (2) comparison of a 1-step imputation approach, where 50 k genotypes are directly imputed to sequence level, to a 2-step imputation approach that used an intermediate step imputing first to 700 k and subsequently to sequence level, (3) the software tools Beagle and Minimac, and (4) the size and composition of the reference panel for imputation. Analyses were performed for 30 DSN and 30 Holstein Frisian cattle available from the 1000 Bull Genomes Project. Imputation accuracy was assessed using a leave-one-out cross validation procedure. We observed that phasing of the target populations and the reference panels affects the imputation accuracy significantly. Minimac reached higher accuracy when imputing using small reference panels, while Beagle performed better with larger reference panels. In contrast to previous research, we found that when a low number of animals is available at the intermediate imputation step, the 1-step imputation approach yielded higher imputation accuracy compared to a 2-step imputation. Overall, the size of the reference panel for imputation is the most important factor leading to higher imputation accuracy, although using a larger reference panel consisting of a related but different breed (Holstein Frisian) significantly reduced imputation accuracy. Our findings provide specific recommendations for populations with a limited number of high density genotyped or sequenced animals available such as DSN. The overall recommendation when imputing a small population are to (1) use a large reference panel of the same breed, (2) use a large reference panel consisting of diverse breeds, or (3) when a large reference panel is not available, we recommend using a smaller same breed reference panel without including a different related breed.", "gemini": "Việc tính toán từ kiểu gen chip SNP có mật độ thấp đến trình tự toàn bộ hệ gen là một cách tiếp cận đã được thiết lập để tạo ra kiểu gen mật độ cao cho nhiều cá thể. Độ chính xác của tính toán phụ thuộc vào nhiều yếu tố và đối với quần thể gia súc nhỏ như gia súc Black Pied đen Đức (DSN) đang có nguy cơ tuyệt chủng, việc xác định chiến lược tính toán tối ưu đặc biệt khó khăn vì chỉ có một số lượng nhỏ kiểu gen mật độ cao có sẵn. Trong bài báo này, độ chính xác của tính toán đã được khám phá liên quan đến (1) pha của quần thể mục tiêu và bảng tham chiếu để tính toán, (2) so sánh phương pháp tính toán 1 bước, trong đó kiểu gen 50 k được tính toán trực tiếp đến trình tự mức độ, với phương pháp tính toán 2 bước sử dụng bước trung gian tính toán trước tiên ở mức 700 k và sau đó đến trình tự mức độ, (3) các công cụ phần mềm Beagle và Minimac và (4) kích thước và thành phần của bảng tham chiếu để tính toán. Các phân tích đã được thực hiện cho 30 DSN và 30 gia súc Holstein Frisian có sẵn từ Dự án 1000 Bull Genomes. Độ chính xác của tính toán được đánh giá bằng quy trình xác thực chéo bỏ một lần. Chúng tôi quan sát thấy rằng pha của quần thể mục tiêu và bảng tham chiếu ảnh hưởng đáng kể đến độ chính xác của tính toán. Minimac đạt độ chính xác cao hơn khi tính toán bằng cách sử dụng các bảng tham chiếu nhỏ, trong khi Beagle hoạt động tốt hơn với các bảng tham chiếu lớn hơn. Trái ngược với các nghiên cứu trước đây, chúng tôi thấy rằng khi có một số lượng nhỏ động vật ở bước tính toán trung gian, phương pháp tính toán 1 bước mang lại độ chính xác tính toán cao hơn so với tính toán 2 bước. Nhìn chung, kích thước của bảng tham chiếu để tính toán là yếu tố quan trọng nhất dẫn đến độ chính xác tính toán cao hơn, mặc dù việc sử dụng bảng tham chiếu lớn hơn bao gồm một giống liên quan nhưng khác (Holstein Frisian) làm giảm đáng kể độ chính xác tính toán. Những phát hiện của chúng tôi đưa ra các khuyến nghị cụ thể cho các quần thể có số lượng động vật được xác định kiểu gen hoặc giải trình tự mật độ cao hạn chế như DSN. Khuyến nghị chung khi tính toán một quần thể nhỏ là (1) sử dụng bảng tham chiếu lớn cùng giống, (2) sử dụng bảng tham chiếu lớn bao gồm các giống khác nhau hoặc (3) khi không có bảng tham chiếu lớn, chúng tôi khuyên bạn nên sử dụng bảng tham chiếu cùng giống nhỏ hơn mà không bao gồm giống liên quan khác."} +{"idx": 1910051, "en": "[This corrects the article DOI: 10.3389/fgene.2018.00657.].", "gemini": "[Điều này sửa bài báo DOI: 10.3389/fgene.2018.00657.]."} +{"idx": 1910052, "en": "The biological markers of aging used to predict physical health status in older people are of great interest. Telomere shortening, which occurs during the process of cell replication, was initially considered a promising biomarker for the prediction of age and age-related outcomes (e.g., diseases, longevity). However, the high instability in detection and low correlation with age-related outcomes limit the extension of telomere length to the field of prediction. Currently, a growing number of studies have shown that dynamic DNA methylation throughout human lifetime exhibits strong correlation with age and age-related outcomes. Indeed, many researchers have built age prediction models with high accuracy based on age-dependent methylation changes in certain CpG loci. For now, DNA methylation based on epigenetic clocks, namely epigenetic or DNA methylation age, serves as a new standard to track chronological age and predict biological age. Measures of age acceleration (Δage, DNA methylation age - chronological age) have been developed to assess the health status of a person. In addition, there is evidence that an accelerated epigenetic age exists in patients with certain age-related diseases (e.g., Alzheimer's disease, cardiovascular disease). In this review, we provide an overview of the dynamic signatures of DNA methylation during aging and emphasize its practical utility in the prediction of various age-related outcomes.", "gemini": "Các dấu ấn sinh học của quá trình lão hóa được sử dụng để dự đoán tình trạng sức khỏe thể chất ở người cao tuổi rất được quan tâm. Sự rút ngắn telomere, xảy ra trong quá trình sao chép tế bào, ban đầu được coi là một dấu ấn sinh học đầy hứa hẹn để dự đoán tuổi và các kết cục liên quan đến tuổi (ví dụ: bệnh tật, tuổi thọ). Tuy nhiên, sự không ổn định cao trong phát hiện và mối tương quan thấp với các kết cục liên quan đến tuổi hạn chế việc mở rộng độ dài telomere vào lĩnh vực dự đoán. Hiện nay, ngày càng có nhiều nghiên cứu chỉ ra rằng quá trình methyl hóa DNA động trong suốt cuộc đời của con người có mối tương quan mạnh với tuổi và các kết cục liên quan đến tuổi. Thật vậy, nhiều nhà nghiên cứu đã xây dựng các mô hình dự đoán tuổi với độ chính xác cao dựa trên các thay đổi về methyl hóa phụ thuộc vào tuổi ở một số vị trí CpG nhất định. Hiện tại, quá trình methyl hóa DNA dựa trên đồng hồ biểu sinh, cụ thể là tuổi biểu sinh hoặc tuổi methyl hóa DNA, đóng vai trò như một tiêu chuẩn mới để theo dõi tuổi theo thời gian và dự đoán tuổi sinh học. Các biện pháp tăng tốc độ tuổi (Δage, tuổi methyl hóa DNA - tuổi theo thời gian) đã được phát triển để đánh giá tình trạng sức khỏe của một người. Ngoài ra, có bằng chứng cho thấy tuổi biểu sinh tăng tốc tồn tại ở những bệnh nhân mắc một số bệnh liên quan đến tuổi (ví dụ: bệnh Alzheimer, bệnh tim mạch). Trong bài đánh giá này, chúng tôi cung cấp một cái nhìn tổng quan về các đặc điểm động của quá trình methyl hóa DNA trong quá trình lão hóa và nhấn mạnh tính hữu ích thực tế của nó trong việc dự đoán các kết cục khác nhau liên quan đến tuổi."} +{"idx": 1910053, "en": "Dystrophinopathies are a group of neuromuscular disorders resulting from mutations in DMD, including Duchenne muscular dystrophy (DMD), intermediate muscular dystrophy (IMD), and Becker muscular dystrophy (BMD). Herein, we present the characteristics of small mutations in Chinese patients with dystrophinopathies, and explore genotype-phenotype correlations. In our cohort, 115 patients with small mutations (18.49% of all patients) were included and DMD mutations were detected by either Sanger (53.91%) or next generation sequencing (46.09%). In total, 106 small mutations were detected, 28 of which (26.42%) had not been reported previously. The most common mutations were nonsense mutations (52.17%), followed by splicing (24.35%), frameshift (17.39%), and missense mutations (5.22%), in addition to a single untranslated region mutation (0.87%). We discovered distinct mutation characteristics in our patients, such as different positional distributions, indicating different exon skipping therapy strategies for small mutations in Chinese patients. Almost all patients (96.51%) with truncating or missense mutations, were covered by triple/double/single-exon skipping therapy; the most frequent single-exon skipping strategy was skipping exon 32, applicable for 8.51% of patients. Furthermore, splicing classification grades were correlated with phenotypes in nonsense mutations (P < 0.001), and serum creatinine levels differed significantly between DMD/IMD and BMD for patients ≤ 16 years old (P = 0.002). These observations can further aid prognostic judgment and guide treatment. In conclusion, the mutation characteristics and genotype-phenotype correlations in Chinese patients with dystrophinopathies and small mutations could provide insights into the molecular mechanisms of pathogenesis, diagnosis, and treatment designs.", "gemini": "Dystrophinopathies là một nhóm các rối loạn thần kinh cơ do đột biến ở DMD, bao gồm loạn dưỡng cơ Duchenne (DMD), loạn dưỡng cơ trung gian (IMD) và loạn dưỡng cơ Becker (BMD). Trong bài này, chúng tôi trình bày các đặc điểm của các đột biến nhỏ ở bệnh nhân Trung Quốc mắc bệnh dystrophinopathy và khám phá mối tương quan giữa kiểu gen và kiểu hình. Trong nhóm bệnh nhân của chúng tôi, 115 bệnh nhân có đột biến nhỏ (18,49% trong tổng số bệnh nhân) đã được đưa vào và các đột biến DMD được phát hiện bằng phương pháp Sanger (53,91%) hoặc giải trình tự thế hệ tiếp theo (46,09%). Tổng cộng, 106 đột biến nhỏ đã được phát hiện, 28 trong số đó (26,42%) chưa từng được báo cáo trước đây. Các đột biến phổ biến nhất là đột biến vô nghĩa (52,17%), tiếp theo là đột biến cắt nối (24,35%), dịch khung (17,39%) và đột biến sai nghĩa (5,22%), ngoài một đột biến vùng không dịch mã (0,87%). Chúng tôi phát hiện ra các đặc điểm đột biến khác biệt ở bệnh nhân của mình, chẳng hạn như các phân bố vị trí khác nhau, chỉ ra các chiến lược điều trị bỏ qua exon khác nhau cho các đột biến nhỏ ở bệnh nhân Trung Quốc. Hầu hết tất cả các bệnh nhân (96,51%) có đột biến cắt cụt hoặc sai nghĩa đều được điều trị bằng liệu pháp bỏ qua exon ba/hai/đơn; chiến lược bỏ qua exon đơn phổ biến nhất là bỏ qua exon 32, áp dụng cho 8,51% bệnh nhân. Hơn nữa, các loại phân loại cắt nối có liên quan đến kiểu hình trong các đột biến vô nghĩa (P < 0,001) và nồng độ creatinine huyết thanh khác biệt đáng kể giữa DMD/IMD và BMD đối với bệnh nhân ≤ 16 tuổi (P = 0,002). Những quan sát này có thể hỗ trợ thêm cho việc đánh giá tiên lượng và hướng dẫn điều trị. Tóm lại, các đặc điểm đột biến và mối tương quan giữa kiểu gen và kiểu hình ở bệnh nhân Trung Quốc mắc bệnh dystrophinopathy và các đột biến nhỏ có thể cung cấp thông tin chi tiết về cơ chế phân tử của sinh bệnh, chẩn đoán và thiết kế điều trị."} +{"idx": 1910054, "en": "Histone modifications are associated with alternative splicing. It has been suggested that histone modifications act in combinational patterns in gene expression regulation. However, how they interact with each other and what is their casual relationships in the process of RNA splicing remain unclear. In this study, the combinatorial patterns of 38 kinds of histone modifications in the exon skipping event of the CD4+ T cell were analyzed by constructing Bayesian networks. Distinct combinatorial patterns of histone modifications that illustrating their casual relationships were observed in excluded/included exons and the surrounding intronic regions. The Bayesian networks also indicate that some histone modifications directly correlate with RNA splicing. We anticipate that this work could provide novel insights into the effects of histone modifications on RNA splicing regulation.", "gemini": "Các sửa đổi histone có liên quan đến sự ghép nối thay thế. Người ta cho rằng các sửa đổi histone hoạt động theo các kiểu kết hợp trong quá trình điều hòa biểu hiện gen. Tuy nhiên, cách chúng tương tác với nhau và mối quan hệ nhân quả của chúng trong quá trình ghép nối RNA vẫn chưa rõ ràng. Trong nghiên cứu này, các kiểu kết hợp của 38 loại sửa đổi histone trong sự kiện bỏ qua exon của tế bào T CD4+ đã được phân tích bằng cách xây dựng các mạng Bayesian. Các kiểu kết hợp riêng biệt của các sửa đổi histone minh họa mối quan hệ nhân quả của chúng được quan sát thấy ở các exon bị loại trừ/bao gồm và các vùng intron xung quanh. Các mạng Bayesian cũng chỉ ra rằng một số sửa đổi histone có mối tương quan trực tiếp với quá trình ghép nối RNA. Chúng tôi dự đoán rằng công trình này có thể cung cấp những hiểu biết mới về tác động của các sửa đổi histone đối với quá trình điều hòa ghép nối RNA."} +{"idx": 1910055, "en": "A bioinspired adaptive model, developed by means of a spiking neural network made of thousands of artificial neurons, has been leveraged to control a humanoid NAO robot in real time. The learning properties of the system have been challenged in a classic cerebellum-driven paradigm, a perturbed upper limb reaching protocol. The neurophysiological principles used to develop the model succeeded in driving an adaptive motor control protocol with baseline, acquisition, and extinction phases. The spiking neural network model showed learning behaviours similar to the ones experimentally measured with human subjects in the same task in the acquisition phase, while resorted to other strategies in the extinction phase. The model processed in real-time external inputs, encoded as spikes, and the generated spiking activity of its output neurons was decoded, in order to provide the proper correction on the motor actuators. Three bidirectional long-term plasticity rules have been embedded for different connections and with different time scales. The plasticities shaped the firing activity of the output layer neurons of the network. In the perturbed upper limb reaching protocol, the neurorobot successfully learned how to compensate for the external perturbation generating an appropriate correction. Therefore, the spiking cerebellar model was able to reproduce in the robotic platform how biological systems deal with external sources of error, in both ideal and real (noisy) environments.", "gemini": "Một mô hình thích ứng được lấy cảm hứng từ sinh học, được phát triển bằng cách sử dụng mạng nơ-ron gai gồm hàng nghìn nơ-ron nhân tạo, đã được sử dụng để điều khiển rô-bốt NAO hình người theo thời gian thực. Các đặc tính học tập của hệ thống đã bị thách thức trong một mô hình cổ điển do tiểu não điều khiển, một giao thức tiếp cận chi trên bị nhiễu. Các nguyên tắc sinh lý thần kinh được sử dụng để phát triển mô hình đã thành công trong việc thúc đẩy một giao thức điều khiển vận động thích ứng với các giai đoạn cơ sở, tiếp thu và tuyệt chủng. Mô hình mạng nơ-ron gai cho thấy các hành vi học tập tương tự như những hành vi được đo lường bằng thực nghiệm với đối tượng là người trong cùng một nhiệm vụ ở giai đoạn tiếp thu, trong khi sử dụng các chiến lược khác ở giai đoạn tuyệt chủng. Mô hình xử lý các đầu vào bên ngoài theo thời gian thực, được mã hóa dưới dạng các gai, và hoạt động gai được tạo ra của các nơ-ron đầu ra của nó đã được giải mã, để cung cấp sự hiệu chỉnh thích hợp cho các bộ truyền động của động cơ. Ba quy tắc dẻo dai dài hạn song phương đã được nhúng vào cho các kết nối khác nhau và với các thang thời gian khác nhau. Tính dẻo đã định hình hoạt động bắn của các nơ-ron lớp đầu ra của mạng. Trong giao thức tiếp cận chi trên bị nhiễu, neurorobot đã học thành công cách bù đắp cho nhiễu bên ngoài tạo ra sự hiệu chỉnh thích hợp. Do đó, mô hình tiểu não gai có thể tái tạo trên nền tảng rô-bốt cách các hệ thống sinh học xử lý các nguồn lỗi bên ngoài, trong cả môi trường lý tưởng và thực (nhiễu)."} +{"idx": 1910056, "en": "Background In the United States, patients wear a one-piece, reusable cloth gown during colonoscopy procedures. Many patients report embarrassment related to bodily exposure during colonoscopy. This may limit participation in colorectal cancer screening programs. Aims To assess whether the use of a novel, disposable patient garment (Privacy Pants, Jackson, MS), which increases patient coverage, can reduce embarrassment related to bodily exposure and increase colonoscopy acceptance rates. Methods Patients were offered a novel gown, and they completed questionnaires before and after colonoscopy. Results A total of 120 patients participated. 54% were female and 82% were Caucasian. The novel gown had high overall satisfaction (8.3) and was associated with a sense of respect during the procedure (9.4). 67% (80) of the patients had a prior colonoscopy, and of these, 76% would request a novel gown over a traditional gown for future procedures. Among all study participants, a high rate of acceptability for repeat colonoscopy if recommended by their doctors was reported (mean of 9.4). Nonwhites were more likely to have a concern for embarrassment addressed by using novel gowns as compared to whites (P value 0.02). Conclusion All participants, particularly women and nonwhite participants, reported high rates of respect and satisfaction and decrease in embarrassment utilizing the novel gown during colonoscopy. Patients who had prior colonoscopy with a traditional gown preferred the novel garment. A novel procedure gown may enhance colonoscopy acceptance by minimizing embarrassment.", "gemini": "Bối cảnh Tại Hoa Kỳ, bệnh nhân mặc áo choàng vải một mảnh, có thể tái sử dụng trong các thủ thuật nội soi đại tràng. Nhiều bệnh nhân báo cáo cảm thấy xấu hổ liên quan đến việc tiếp xúc cơ thể trong quá trình nội soi đại tràng. Điều này có thể hạn chế sự tham gia vào các chương trình sàng lọc ung thư đại trực tràng. Mục đích Đánh giá xem việc sử dụng một loại áo choàng bệnh nhân dùng một lần mới (Quần bảo vệ sự riêng tư, Jackson, MS), giúp tăng phạm vi che phủ cho bệnh nhân, có thể làm giảm sự xấu hổ liên quan đến việc tiếp xúc cơ thể và tăng tỷ lệ chấp nhận nội soi đại tràng hay không. Phương pháp Bệnh nhân được cung cấp một loại áo choàng mới và họ đã hoàn thành các bảng câu hỏi trước và sau khi nội soi đại tràng. Kết quả Tổng cộng có 120 bệnh nhân tham gia. 54% là nữ và 82% là người da trắng. Áo choàng mới có mức độ hài lòng chung cao (8,3) và gắn liền với cảm giác được tôn trọng trong suốt quá trình (9,4). 67% (80) bệnh nhân đã nội soi đại tràng trước đó và trong số những bệnh nhân này, 76% sẽ yêu cầu một chiếc áo choàng mới thay cho áo choàng truyền thống cho các thủ thuật trong tương lai. Trong số tất cả những người tham gia nghiên cứu, tỷ lệ chấp nhận nội soi đại tràng lặp lại nếu được bác sĩ khuyến nghị là cao (trung bình 9,4). Người không phải da trắng có nhiều khả năng quan tâm đến sự xấu hổ được giải quyết bằng cách sử dụng áo choàng mới so với người da trắng (Giá trị P là 0,02). Kết luận Tất cả những người tham gia, đặc biệt là phụ nữ và những người không phải da trắng, đều báo cáo tỷ lệ tôn trọng và hài lòng cao và giảm sự xấu hổ khi sử dụng áo choàng mới trong quá trình nội soi đại tràng. Những bệnh nhân đã nội soi đại tràng trước đó bằng áo choàng truyền thống thích loại áo choàng mới. Một loại áo choàng thủ thuật mới có thể nâng cao khả năng chấp nhận nội soi đại tràng bằng cách giảm thiểu sự xấu hổ."} +{"idx": 1910057, "en": "This study investigated antipathogenic efficacy of a polyherbal wound-healing formulation Herboheal against three multidrug-resistant strains of gram-negative bacterial pathogens associated with wound infections. Herboheal was evaluated for its quorum-modulatory potential against three different human-pathogenic bacteria, first in vitro through the broth dilution assay and then in vivo in the model host Caenorhabditis elegans. Herboheal at ≥0.1% v/v was able to inhibit (19-55%) in vitro production of quorum sensing-regulated pigments in all these bacteria and seemed to interfere with bacterial quorum sensing by acting as a signal-response inhibitor. This formulation could compromise haemolytic activity of all three bacteria by ∼18-69% and induced their catalase activity by ∼8-21%. Herboheal inhibited P. aeruginosa biofilm formation up to 40%, reduced surface hydrophobicity of P. aeruginosa cells by ∼9%, and also made them (25%) more susceptible to lysis by human serum. Antibiotic susceptibility of all three bacteria was modulated owing to pretreatment with Herboheal. Exposure of these test pathogens to Herboheal (≥0.025% v/v) effectively reduced their virulence towards the nematode Caenorhabditis elegans. Repeated subculturing of P. aeruginosa on the Herboheal-supplemented growth medium did not induce resistance to Herboheal in this mischievous pathogen, and this polyherbal extract was also found to exert a post-extract effect on P. aeruginosa, wherein virulence of the Herboheal-unexposed daughter cultures, of the Herboheal-exposed parent culture, was also found to be attenuated. Overall, this study indicates Herboheal formulation to be an effective antipathogenic preparation and validates its indicated traditional therapeutic use as a wound-care formulation.", "gemini": "Nghiên cứu này đã điều tra hiệu quả chống mầm bệnh của một chế phẩm thảo dược chữa lành vết thương Herboheal đối với ba chủng vi khuẩn Gram âm đa kháng thuốc liên quan đến nhiễm trùng vết thương. Herboheal được đánh giá về khả năng điều hòa số lượng vi khuẩn đối với ba loại vi khuẩn gây bệnh ở người khác nhau, đầu tiên là trong ống nghiệm thông qua xét nghiệm pha loãng dịch nuôi cấy và sau đó là trong cơ thể sống ở vật chủ mô hình Caenorhabditis elegans. Herboheal ở nồng độ ≥0,1% v/v có khả năng ức chế (19-55%) sản xuất sắc tố do cảm ứng số lượng vi khuẩn trong ống nghiệm ở tất cả các loại vi khuẩn này và dường như can thiệp vào cảm ứng số lượng vi khuẩn bằng cách hoạt động như một chất ức chế phản ứng tín hiệu. Chế phẩm này có thể làm giảm hoạt tính tan máu của cả ba loại vi khuẩn khoảng 18-69% và gây ra hoạt tính catalase của chúng khoảng 8-21%. Herboheal ức chế sự hình thành màng sinh học của P. aeruginosa lên đến 40%, làm giảm độ kỵ nước bề mặt của các tế bào P. aeruginosa khoảng 9% và cũng làm cho chúng (25%) dễ bị ly giải hơn bởi huyết thanh người. Khả năng nhạy cảm với kháng sinh của cả ba loại vi khuẩn đều được điều chỉnh do được xử lý trước bằng Herboheal. Việc tiếp xúc với các mầm bệnh thử nghiệm này với Herboheal (≥0,025% v/v) đã làm giảm hiệu quả độc lực của chúng đối với giun tròn Caenorhabditis elegans. Việc nuôi cấy phụ nhiều lần P. aeruginosa trên môi trường tăng trưởng bổ sung Herboheal không gây ra tình trạng kháng Herboheal ở mầm bệnh gây hại này và chiết xuất thảo dược này cũng được phát hiện có tác dụng sau khi chiết xuất đối với P. aeruginosa, trong đó độc lực của các nuôi cấy con gái không tiếp xúc với Herboheal, của nuôi cấy mẹ tiếp xúc với Herboheal, cũng được phát hiện là giảm. Nhìn chung, nghiên cứu này chỉ ra rằng chế phẩm Herboheal là một chế phẩm chống mầm bệnh hiệu quả và xác nhận việc sử dụng liệu pháp truyền thống được chỉ định của chế phẩm này như một chế phẩm chăm sóc vết thương."} +{"idx": 1910058, "en": "Death and severe morbidity attributable to anesthesia are commonly associated with failed difficult airway management. When an airway emergency develops, immediate access to difficult airway equipment is critical for implementation of rescue strategies. Previously, national expert consensus guidelines have provided only limited guidance for the design and setup of a difficult airway trolley. The overarching aim of the current work was to create a dedicated difficult airway trolley (for patients>12 years old) for use in anesthesia theatres, intensive care units, and emergency departments. A systematic literature search was performed, using the PubMed, Embase, and Google Scholar search engines. Based on evidence presented in 11 national or international guidelines, and peer-reviewed journals, we present and outline a difficult airway trolley organized to accommodate sequential progression through a four-step difficult airway algorithm. The contents of the top four drawers correspond to specific steps in the airway algorithm (A = intubation, B = oxygenation via a supraglottic airway device, C = facemask ventilation, and D = emergency invasive airway access). Additionally, specialized airway equipment may be included in the fifth drawer of the proposed difficult airway trolley, thus enabling widespread use. A logically designed, guideline-based difficult airway trolley is a vital resource for any clinician involved in airway management and may aid the adherence to difficult airway algorithms during evolving airway emergencies. Future research examining the availability of rescue airway devices in various clinical settings, and simulation studies comparing different types of difficult airway trolleys, are encouraged.", "gemini": "Tử vong và bệnh tật nghiêm trọng do gây mê thường liên quan đến việc không xử lý được tình trạng khó đặt nội khí quản. Khi tình trạng khẩn cấp về đường thở xảy ra, việc tiếp cận ngay lập tức với thiết bị hỗ trợ đường thở khó là rất quan trọng để thực hiện các chiến lược cấp cứu. Trước đây, các hướng dẫn đồng thuận của chuyên gia quốc gia chỉ cung cấp hướng dẫn hạn chế về thiết kế và thiết lập xe đẩy hỗ trợ đường thở khó. Mục đích chính của công trình hiện tại là tạo ra một xe đẩy hỗ trợ đường thở khó chuyên dụng (dành cho bệnh nhân> 12 tuổi) để sử dụng trong các phòng gây mê, khoa hồi sức tích cực và khoa cấp cứu. Một tìm kiếm tài liệu có hệ thống đã được thực hiện, sử dụng các công cụ tìm kiếm PubMed, Embase và Google Scholar. Dựa trên các bằng chứng được trình bày trong 11 hướng dẫn quốc gia hoặc quốc tế và các tạp chí được bình duyệt, chúng tôi trình bày và phác thảo một xe đẩy hỗ trợ đường thở khó được tổ chức để phù hợp với tiến trình tuần tự thông qua thuật toán hỗ trợ đường thở khó bốn bước. Nội dung của bốn ngăn kéo trên cùng tương ứng với các bước cụ thể trong thuật toán đường thở (A = đặt nội khí quản, B = cung cấp oxy thông qua thiết bị đường thở thanh quản, C = thông khí bằng mặt nạ và D = tiếp cận đường thở xâm lấn khẩn cấp). Ngoài ra, thiết bị hỗ trợ đường thở chuyên dụng có thể được đưa vào ngăn kéo thứ năm của xe đẩy hỗ trợ đường thở khó được đề xuất, do đó cho phép sử dụng rộng rãi. Một xe đẩy hỗ trợ đường thở khó được thiết kế hợp lý, dựa trên hướng dẫn là một nguồn lực quan trọng cho bất kỳ bác sĩ lâm sàng nào tham gia vào việc quản lý đường thở và có thể hỗ trợ tuân thủ các thuật toán hỗ trợ đường thở khó trong quá trình tiến triển của các trường hợp khẩn cấp về đường thở. Nghiên cứu trong tương lai kiểm tra tính khả dụng của các thiết bị hỗ trợ đường thở trong các bối cảnh lâm sàng khác nhau và các nghiên cứu mô phỏng so sánh các loại xe đẩy hỗ trợ đường thở khó khác nhau được khuyến khích."} +{"idx": 1910059, "en": "Lavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. angustifolia essential oil was analyzed using gas chromatography-mass spectrometry methods and showed predominance of Eucalyptol (52.36%), Camphor (11.91%), gamma-terpinene (8.775%) and endoborneol (7.585%). Limit test at 2000 mg/kg dose was used for L. angustifolia essential oil acute toxicity test and revealed LD50 value was higher than 2000 mg/kg. For subacute toxicity study 2000mg/kg was given orally to each mouse for 21 days. The result demonstrated no significant changes (p > 0.05) in the body weights, and biochemical parameters, gross abnormalities, water, and food intake were observed. No macroscopic changes were seen in the histopathology analysis of kidneys and livers. For skin irritation test shaved rabbit skin was treated with 10% ointment formulation. Ointment of L. angustifolia oil did not affect mice skin. Generally, this toxicity study demonstrated that L. angustifolia essential oil is nontoxic.", "gemini": "Lavandula angustifolia được sử dụng trong y học dân gian và truyền thống của Quận Ankober, Bắc Trung Ethiopia, để điều trị một số bệnh ở gia súc và người. Nghiên cứu độc tính này nhằm mục đích điều tra độc tính đường uống của tinh dầu L. angustifolia ở chuột và kích ứng da ở thỏ. Tinh dầu L. angustifolia được phân tích bằng phương pháp sắc ký khí khối phổ và cho thấy sự chiếm ưu thế của Eucalyptol (52,36%), Long não (11,91%), gamma-terpinene (8,775%) và endoborneol (7,585%). Xét nghiệm giới hạn ở liều 2000 mg/kg đã được sử dụng cho xét nghiệm độc tính cấp tính của tinh dầu L. angustifolia và cho thấy giá trị LD50 cao hơn 2000 mg/kg. Đối với nghiên cứu độc tính bán cấp, 2000mg/kg được cho uống cho mỗi con chuột trong 21 ngày. Kết quả cho thấy không có thay đổi đáng kể (p> 0,05) về trọng lượng cơ thể và các thông số sinh hóa, bất thường về tổng thể, lượng nước và thức ăn nạp vào. Không thấy thay đổi vĩ mô nào trong phân tích mô bệnh học của thận và gan. Đối với thử nghiệm kích ứng da, da thỏ đã cạo được xử lý bằng chế phẩm thuốc mỡ 10%. Thuốc mỡ dầu L. angustifolia không ảnh hưởng đến da chuột. Nhìn chung, nghiên cứu độc tính này chứng minh rằng tinh dầu L. angustifolia không độc."} +{"idx": 1910060, "en": "This report describes two maneuvers in different steps of phaco surgery in a case with hard cataract, which provide debulking of the central dense nucleus and prevents posterior capsule rupture (PCR). In the current case, clear corneal incisions were created, and anterior chamber was filled with ophthalmic viscosurgical device (OVD). Anterior capsule was punctured, and capsulorhexis was completed. Nucleus was cracked into two halves following vertical groove formation. Core nucleus was hollowed sideward bilaterally in the capsular bag. Nuclear halves were removed from capsular bag, and each one was pushed to one side on the iris plane. Capsular bag was inflated with OVD, and intraocular lens (IOL) was implanted. Nuclear halves were removed in confidence. The presented maneuvers initially reduce dense nucleus load in the safe zone and allow surgeon to use IOL as a barrier to protect floppy posterior capsule from early steps of the surgery.", "gemini": "Báo cáo này mô tả hai thao tác ở các bước khác nhau của phẫu thuật phaco trên một trường hợp đục thủy tinh thể cứng, giúp giảm khối lượng nhân cứng ở trung tâm và ngăn ngừa vỡ bao sau (PCR). Trong trường hợp hiện tại, các vết rạch giác trong được tạo ra và buồng trước được làm đầy bằng thiết bị phẫu thuật nội soi (OVD). Bao trước được chọc thủng và tạo vạt bao trước hoàn chỉnh. Nhân được chia thành hai nửa theo rãnh dọc. Nhân cứng được khoét rỗng hai bên trong bao. Các nửa nhân được lấy ra khỏi bao và mỗi nửa được đẩy sang một bên trên mặt phẳng mống mắt. Bao được bơm căng bằng OVD và thủy tinh thể nhân tạo (IOL) được cấy vào. Các nửa nhân được lấy ra một cách an toàn. Các thao tác được trình bày ban đầu làm giảm tải trọng nhân cứng trong vùng an toàn và cho phép bác sĩ phẫu thuật sử dụng IOL như một rào cản để bảo vệ bao sau mềm khỏi các bước đầu của phẫu thuật."} +{"idx": 1910061, "en": "Objective To investigate the effects of mesenchymal stem cells (MSCs) coated by the extracellular matrix (ECM) on wound healing in diabetic rats. Methods Mesenchymal stem cells were cocultured with ECM. Cell viabilities were evaluated using MTT assay. The diabetes model was established using both STZ and high-glucose/fat methods in SD rats. A wound area was made on the middle of the rats' back. MSCs or ECM-MSCs were used to treat the rats. HE staining and CD31 immunohistochemistry were used to detect the skin thickness and angiogenesis. Western blotting and qRT-PCR were conducted to determine the level of VEGF-α, PDGF, and EGF. Results It was observed that treatment of ECM had no significant effects on the cell viability of ECM-MSCs. Wound area assay showed that both MSCs and ECM-MSCs could enhance the wound healing of diabetic rats and ECM-MSCs could further promote the effects. Both MSCs and ECM-MSCs could enhance angiogenesis and epithelialization of the wounds, as well as the expression of VEGF-α, PDGF, and EGF in wound tissues, while ECM-MSC treatment showed more obvious effects. Conclusion Mesenchymal stem cells coated by the extracellular matrix could promote wound healing in diabetic rats. Our study may offer a novel therapeutic method for impaired diabetic wound healing.", "gemini": "Mục tiêu Nghiên cứu tác dụng của tế bào gốc trung mô (MSC) được phủ bởi ma trận ngoại bào (ECM) đối với quá trình lành vết thương ở chuột mắc bệnh tiểu đường. Phương pháp Tế bào gốc trung mô được nuôi đồng thời với ECM. Khả năng sống của tế bào được đánh giá bằng xét nghiệm MTT. Mô hình bệnh tiểu đường được thiết lập bằng cả phương pháp STZ và phương pháp glucose/chất béo cao ở chuột SD. Một vùng vết thương được tạo ra ở giữa lưng chuột. MSC hoặc ECM-MSC được sử dụng để điều trị cho chuột. Nhuộm HE và miễn dịch mô học CD31 được sử dụng để phát hiện độ dày da và quá trình hình thành mạch máu. Điện di Western và qRT-PCR được thực hiện để xác định mức độ VEGF-α, PDGF và EGF. Kết quả Quan sát thấy việc xử lý ECM không có tác dụng đáng kể đến khả năng sống của tế bào ECM-MSC. Xét nghiệm diện tích vết thương cho thấy cả MSC và ECM-MSC đều có thể thúc đẩy quá trình lành vết thương ở chuột mắc bệnh tiểu đường và ECM-MSC có thể thúc đẩy thêm các tác dụng. Cả MSC và ECM-MSC đều có thể thúc đẩy quá trình hình thành mạch máu và biểu mô hóa vết thương, cũng như sự biểu hiện của VEGF-α, PDGF và EGF trong mô vết thương, trong khi điều trị bằng ECM-MSC cho thấy tác dụng rõ ràng hơn. Kết luận Tế bào gốc trung mô được phủ bởi ma trận ngoại bào có thể thúc đẩy quá trình lành vết thương ở chuột mắc bệnh tiểu đường. Nghiên cứu của chúng tôi có thể cung cấp một phương pháp điều trị mới cho tình trạng lành vết thương ở bệnh nhân tiểu đường bị suy yếu."} +{"idx": 1910062, "en": "Background Honokiol is a low-molecular-weight natural product and has been reported to exhibit anti-inflammatory activity. Objectives Our study aimed to investigate the influence of honokiol on sepsis-induced acute kidney injury (AKI) in a mouse model. Material and methods A cecal ligation and puncture (CLP) surgical operation was performed to establish a sepsis-induced acute kidney injury model in mice. Renal histomorphological analysis was performed with periodic acid-Schiff (PAS) staining. The levels of inflammatory markers in serum were measured by ELISA assay. The mRNA and protein levels were assayed by RT-qPCR and western blotting, respectively. Annexin V-FITC/PI staining was used to evaluate glomerular mesangial cell (GMC) apoptosis. Results The results revealed that honokiol significantly increased the survival rate in mice undergoing a CLP operation. Inflammatory cytokines, such as TNF-α, IL-6 and IL-1β, were significantly inhibited in honokiol-treated septic mice compared with the CLP group. In addition, honokiol showed the ability to reverse CLP-induced AKI in septic mice. Furthermore, heme oxygenase-1 (HO-1) expression levels were significantly up-regulated and miR-218-5p was markedly down-regulated in honokiol-treated septic mice as compared to CLP-operated mice. Bioinformatics and experimental measurements showed that HO-1 was a direct target of miR-218-5p. In vitro experiments showed that both honokiol and miR-218-5p inhibitors blocked lipopolysaccharide (LPS)-induced cell growth inhibition and GMC apoptosis by increasing the expression of HO-1. Conclusions Honokiol ameliorated AKI in septic mice and LPS-induced GMC dysfunction, and the underlying mechanism was mediated, at least partially, through the regulation of miR-218-5p/HO-1 signaling.", "gemini": "Bối cảnh Honokiol là một sản phẩm tự nhiên có trọng lượng phân tử thấp và đã được báo cáo là có hoạt tính chống viêm. Mục tiêu Nghiên cứu của chúng tôi nhằm điều tra ảnh hưởng của honokiol đối với tổn thương thận cấp tính (AKI) do nhiễm trùng huyết ở mô hình chuột. Vật liệu và phương pháp Một ca phẫu thuật thắt và chọc manh tràng (CLP) được thực hiện để thiết lập mô hình tổn thương thận cấp tính do nhiễm trùng huyết ở chuột. Phân tích mô học thận được thực hiện bằng phương pháp nhuộm axit periodic-Schiff (PAS). Nồng độ các dấu ấn viêm trong huyết thanh được đo bằng xét nghiệm ELISA. Mức độ mRNA và protein được xác định bằng RT-qPCR và điện di Western, tương ứng. Nhuộm Annexin V-FITC/PI được sử dụng để đánh giá quá trình apoptosis của tế bào cầu thận (GMC). Kết quả Kết quả cho thấy honokiol làm tăng đáng kể tỷ lệ sống sót ở những con chuột trải qua phẫu thuật CLP. Các cytokine gây viêm, như TNF-α, IL-6 và IL-1β, bị ức chế đáng kể ở những con chuột nhiễm trùng huyết được điều trị bằng honokiol so với nhóm CLP. Ngoài ra, honokiol cho thấy khả năng đảo ngược AKI do CLP gây ra ở những con chuột bị nhiễm trùng huyết. Hơn nữa, mức độ biểu hiện heme oxygenase-1 (HO-1) tăng lên đáng kể và miR-218-5p giảm đáng kể ở những con chuột bị nhiễm trùng huyết được điều trị bằng honokiol so với những con chuột được phẫu thuật CLP. Tin sinh học và các phép đo thực nghiệm cho thấy HO-1 là mục tiêu trực tiếp của miR-218-5p. Các thí nghiệm trong ống nghiệm cho thấy cả honokiol và chất ức chế miR-218-5p đều ngăn chặn sự ức chế tăng trưởng tế bào do lipopolysaccharide (LPS) gây ra và quá trình apoptosis của GMC bằng cách tăng biểu hiện của HO-1. Kết luận Honokiol làm giảm AKI ở những con chuột bị nhiễm trùng huyết và rối loạn chức năng GMC do LPS gây ra, và cơ chế cơ bản được trung gian, ít nhất là một phần, thông qua sự điều hòa tín hiệu miR-218-5p/HO-1."} +{"idx": 1910063, "en": "Objectives Irrigation plays a critical role in endodontic treatment. Various single and combined irrigants and irrigation protocols are available. The aim of this study was to evaluate the effect of some common irrigation protocols on the coronal fracture resistance of endodontically treated teeth undergoing bleaching. Materials and Methods After preparation of access cavities in 120 maxillary premolars, the teeth were divided into five groups (n=24) based on the irrigation protocol; G1:2.5% sodium hypochlorite (NaOCl), G2: 2% chlorhexidine (CHX), G3: NaOCl+CHX, G4: NaOCl+ethylenediaminetetraacetic acid (EDTA), G5: NaOCl+EDTA+CHX. Each group was subdivided into 2 subgroups of A: non-bleached (NB) and B: bleached (B). In subgroup B, the teeth underwent in-office and at-home bleaching techniques using 38% hydrogen peroxide and 20% carbamide peroxide gels for 3 weeks. The teeth were restored with composite resin, thermocycled, incubated for 24 hours, and underwent fracture resistance tests. Data were analyzed with one-way analysis of variance (ANOVA) and t-test (α=0.05). Results T-test showed significant differences between every two corresponding groups (P<0.0001). In subgroup A, the minimum fracture resistance was recorded in G1. Also, G2 specimens exhibited a significantly higher fracture resistance compared to G1, G4, and G5. In subgroup B, G2 specimens exhibited a significantly higher fracture resistance compared to G1 and G4 that were irrigated using NaOCl and NaOCl+EDTA. Conclusions It can be concluded that irrigation protocols can affect the coronal fracture resistance of bleached endodontically treated teeth, and specific irrigation protocols can be recommended for teeth undergoing bleaching.", "gemini": "Mục tiêu Quá trình tưới rửa đóng vai trò quan trọng trong điều trị nội nha. Có nhiều loại thuốc rửa và phác đồ rửa đơn lẻ và kết hợp khác nhau. Mục đích của nghiên cứu này là đánh giá tác động của một số phác đồ rửa thông thường đến sức bền gãy ở thân răng của răng đã điều trị nội nha đang tẩy trắng. Vật liệu và phương pháp Sau khi chuẩn bị lỗ tiếp cận ở 120 răng tiền hàm hàm trên, răng được chia thành năm nhóm (n = 24) dựa trên phác đồ rửa; G1: 2,5% natri hypoclorit (NaOCl), G2: 2% chlorhexidine (CHX), G3: NaOCl + CHX, G4: NaOCl + axit ethylenediamintetraacetic (EDTA), G5: NaOCl + EDTA + CHX. Mỗi nhóm được chia thành 2 nhóm nhỏ A: không tẩy trắng (NB) và B: tẩy trắng (B). Ở nhóm phụ B, răng trải qua các kỹ thuật tẩy trắng tại phòng khám và tại nhà sử dụng gel hydro peroxide 38% và gel carbamide peroxide 20% trong 3 tuần. Răng được phục hồi bằng nhựa composite, nung nóng, ủ trong 24 giờ và trải qua các thử nghiệm độ bền gãy. Dữ liệu được phân tích bằng phân tích phương sai một chiều (ANOVA) và kiểm định t (α = 0,05). Kết quả Kiểm định t cho thấy sự khác biệt đáng kể giữa mọi hai nhóm tương ứng (P < 0,0001). Ở nhóm phụ A, độ bền gãy tối thiểu được ghi nhận ở G1. Ngoài ra, các mẫu vật G2 cho thấy độ bền gãy cao hơn đáng kể so với G1, G4 và G5. Ở nhóm phụ B, các mẫu vật G2 cho thấy độ bền gãy cao hơn đáng kể so với G1 và G4 được tưới rửa bằng NaOCl và NaOCl + EDTA. Kết luận Có thể kết luận rằng các phác đồ tưới rửa có thể ảnh hưởng đến sức bền gãy ở thân răng của răng đã điều trị nội nha được tẩy trắng và có thể khuyến cáo các phác đồ tưới rửa cụ thể cho răng đang tẩy trắng."} +{"idx": 1910064, "en": "Objectives This study sought to assess the relationship of oral health literacy (OHL) of parents with the decayed, missing, and filled teeth (DMFT) indices of themselves and their children. Materials and Methods This cross-sectional study was performed on 258 children presenting with their parents to the Pediatric Dentistry and Orthodontics Departments of School of Dentistry, Tehran University of Medical Sciences in 2016. The parents were asked to fill out questionnaires related to OHL, oral health behaviors, and background information. Both parents and children were clinically examined to determine their DMFT indices according to the World Health Organization (WHO) criteria. A backward linear regression model was applied to assess the effect of demographic factors on OHL, behavioral habits, and DMFT. The Pearson's bivariate correlation was used to assess the relationship of OHL, behavioral habits, and DMFT. Results A significant linear correlation was noted between the OHL of the parents and the number of filled teeth in children (P=0.01). Only 48.5% of the parents had adequate OHL. Children whose parents had adequate OHL had a significantly higher number of fillings (P=0.03) and fewer missing teeth (P=0.04). Children whose parents had inadequate or marginal OHL had a significantly lower number of fillings (P=0.01) and more missing teeth (P=0.03). Conclusions Higher OHL of parents seems to be significantly related to the mean DMFT of themselves and their children and enhances their oral health behavior. Programs must be implemented in developing countries, including Iran, to promote the OHL of parents and consequently improve the oral health status of children.", "gemini": "Mục tiêu Nghiên cứu này nhằm đánh giá mối quan hệ giữa kiến thức về sức khỏe răng miệng (OHL) của cha mẹ với chỉ số răng sâu, mất và trám (DMFT) của bản thân họ và con cái họ. Vật liệu và phương pháp Nghiên cứu cắt ngang này được thực hiện trên 258 trẻ em đến khám cùng cha mẹ tại Khoa Răng trẻ em và chỉnh nha, Trường Nha khoa, Đại học Y khoa Tehran vào năm 2016. Các bậc cha mẹ được yêu cầu điền vào bảng câu hỏi liên quan đến OHL, hành vi sức khỏe răng miệng và thông tin cơ bản. Cả cha mẹ và trẻ em đều được khám lâm sàng để xác định chỉ số DMFT của họ theo tiêu chuẩn của Tổ chức Y tế Thế giới (WHO). Mô hình hồi quy tuyến tính ngược đã được áp dụng để đánh giá tác động của các yếu tố nhân khẩu học đối với OHL, thói quen hành vi và DMFT. Hệ số tương quan hai biến của Pearson được sử dụng để đánh giá mối quan hệ giữa OHL, thói quen hành vi và DMFT. Kết quả Có mối tương quan tuyến tính đáng kể giữa OHL của cha mẹ và số răng trám ở trẻ em (P = 0,01). Chỉ có 48,5% cha mẹ có OHL đầy đủ. Trẻ em có cha mẹ có OHL đầy đủ có số răng trám nhiều hơn đáng kể (P = 0,03) và ít răng mất hơn (P = 0,04). Trẻ em có cha mẹ có OHL không đầy đủ hoặc cận biên có số răng trám ít hơn đáng kể (P = 0,01) và nhiều răng mất hơn (P = 0,03). Kết luận OHL cao hơn của cha mẹ dường như có liên quan đáng kể đến chỉ số DMFT trung bình của bản thân họ và con cái họ và cải thiện hành vi sức khỏe răng miệng của họ. Các chương trình phải được triển khai ở các nước đang phát triển, bao gồm cả Iran, để thúc đẩy OHL của cha mẹ và do đó cải thiện tình trạng sức khỏe răng miệng của trẻ em."} +{"idx": 1910065, "en": "Objectives The aim of this study was to evaluate the diagnostic accuracy of different filtrations and slice thicknesses of cone-beam computed tomography (CBCT) in the detection of occlusal caries. Materials and Methods One-hundred teeth were selected for this ex-vivo experimental study. The CBCT images of the teeth were evaluated and scored by two observers in panoramic and cross-sectional views using different slice thicknesses and filtrations. Paired t-test, repeated-measures analysis of variance (ANOVA), and the least significant difference (LSD) test were used to compare the data with the histological gold standard. Receiver operating characteristic (ROC) analysis was used to determine the diagnostic accuracy of each slice thickness and filtration (P<0.05). Results The mean score of true caries detection in cross-sectional views was lower than that in panoramic views (P<0.05). Repeated-measures ANOVA showed a significant difference in the mean of true detections in different thicknesses of cross-sectional views, but this difference was significant only between 5 mm thickness and other thicknesses in panoramic views. On all the views, increasing the thickness decreased the accuracy of caries detection. Repeated-measures ANOVA showed a significant difference between different filtrations; on all the views, increasing the filtration increased the accuracy of caries detection. Conclusions An increase of filtration of CBCT images increases the accuracy of occlusal caries detection; however, an increase in slice thickness results in a lower diagnostic accuracy.", "gemini": "Mục tiêu Mục tiêu của nghiên cứu này là đánh giá độ chính xác chẩn đoán của các phương pháp lọc và độ dày lát cắt khác nhau của chụp cắt lớp vi tính chùm nón (CBCT) trong việc phát hiện sâu răng mặt nhai. Vật liệu và phương pháp Một trăm răng đã được chọn cho nghiên cứu thực nghiệm ngoại sinh này. Hình ảnh CBCT của răng được hai quan sát viên đánh giá và chấm điểm ở chế độ xem toàn cảnh và chế độ xem cắt ngang bằng cách sử dụng các độ dày lát cắt và độ lọc khác nhau. Kiểm định t ghép đôi, phân tích phương sai theo biện pháp lặp lại (ANOVA) và kiểm định chênh lệch ít nhất có ý nghĩa (LSD) đã được sử dụng để so sánh dữ liệu với tiêu chuẩn vàng mô học. Phân tích đặc tính hoạt động của người nhận (ROC) được sử dụng để xác định độ chính xác chẩn đoán của từng độ dày lát cắt và độ lọc (P < 0,05). Kết quả Điểm trung bình phát hiện sâu răng thật ở chế độ xem cắt ngang thấp hơn ở chế độ xem toàn cảnh (P < 0,05). ANOVA theo biện pháp lặp lại cho thấy có sự khác biệt đáng kể về độ phát hiện thực ở các độ dày khác nhau của chế độ xem cắt ngang, nhưng sự khác biệt này chỉ có ý nghĩa giữa độ dày 5 mm và các độ dày khác ở chế độ xem toàn cảnh. Trên tất cả các chế độ xem, việc tăng độ dày làm giảm độ chính xác phát hiện sâu răng. ANOVA theo biện pháp lặp lại cho thấy có sự khác biệt đáng kể giữa các độ lọc khác nhau; trên tất cả các chế độ xem, việc tăng độ lọc làm tăng độ chính xác phát hiện sâu răng. Kết luận Việc tăng độ lọc hình ảnh CBCT làm tăng độ chính xác phát hiện sâu răng mặt nhai; tuy nhiên, việc tăng độ dày lát cắt dẫn đến độ chính xác chẩn đoán thấp hơn."} +{"idx": 1910066, "en": "Objectives The aim of this study was to assess the antibacterial activities of OrthoMTA, RetroMTA, and ProRoot MTA against Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), and Prevotella intermedia (Pi). Materials and Methods Each material was mixed on a glass slab using a spatula and was placed in columns containing the filter membrane of the modified membrane-enclosed immersion test (MEIT) system. The materials were sterilized after setting. The columns containing the sterilized test materials were placed in microcentrifuge tubes containing 500 μl of bacterial suspension. The systems were then incubated at 37°C under anaerobic conditions. After 72 hours, the bacterial growth and concentration (colony-forming unit (CFU)/ml) were assessed. The results were analyzed using one-way analysis of variance (ANOVA) and Tukey's post-hoc test in SPSS 22 software. In all analyses, the differences were considered significant at P<0.05. Results OrthoMTA had the highest antibacterial activity against Pi. The mean number of CFU/ml of Fn in the presence of ProRoot MTA and RetroMTA was significantly lower than that in positive controls. There were significant differences between the antibacterial activities of ProRoot MTA and OrthoMTA against Pg compared to positive controls. Conclusions ProRoot MTA, OrthoMTA, and RetroMTA had similar antibacterial activities against the three evaluated anaerobic endodontic bacteria, except RetroMTA against Pg.", "gemini": "Mục tiêu Mục đích của nghiên cứu này là đánh giá hoạt động kháng khuẩn của OrthoMTA, RetroMTA và ProRoot MTA đối với Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg) và Prevotella intermedia (Pi). Vật liệu và Phương pháp Mỗi vật liệu được trộn trên một phiến thủy tinh bằng thìa và được đặt vào các cột chứa màng lọc của hệ thống thử ngâm trong màng sửa đổi (MEIT). Các vật liệu được khử trùng sau khi đông cứng. Các cột chứa vật liệu thử đã được khử trùng được đặt trong các ống ly tâm chứa 500 μl dịch huyền khuẩn. Sau đó, các hệ thống được ủ ở 37°C trong điều kiện kỵ khí. Sau 72 giờ, đánh giá sự phát triển và nồng độ của vi khuẩn (đơn vị hình thành khuẩn lạc (CFU)/ml). Các kết quả được phân tích bằng phân tích phương sai một chiều (ANOVA) và kiểm định Tukey sau thử nghiệm trong phần mềm SPSS 22. Trong tất cả các phân tích, sự khác biệt được coi là có ý nghĩa ở P < 0,05. Kết quả OrthoMTA có hoạt tính kháng khuẩn cao nhất đối với Pi. Số lượng CFU/ml trung bình của Fn khi có ProRoot MTA và RetroMTA thấp hơn đáng kể so với đối chứng dương tính. Có sự khác biệt đáng kể giữa hoạt tính kháng khuẩn của ProRoot MTA và OrthoMTA đối với Pg so với đối chứng dương tính. Kết luận ProRoot MTA, OrthoMTA và RetroMTA có hoạt động kháng khuẩn tương tự đối với ba loại vi khuẩn nội nha kỵ khí được đánh giá, ngoại trừ RetroMTA đối với Pg."} +{"idx": 1910067, "en": "Objectives This in-vitro study aimed to compare the push-out bond strength of composite resin posts packed into the root canal of primary anterior teeth using two different layering techniques. Materials and Methods Thirty-two primary anterior teeth were randomly divided into two groups. In group 1, after the preparation of post spaces, a posterior composite resin (Filtek P60) was packed in three horizontal layers by a composite condenser instrument with a cylindrical tip using the horizontal layering technique (HLT). In group 2, this was done using a condenser with a conical tip in three funnel-shaped layers according to the funnel-shaped layering technique (FSLT). Next, the specimens were subjected to push-out bond strength testing. Data were analyzed using t-test and the Kaplan-Meier curves. Results The mean±standard deviation (SD) bond strengths of composite resin posts were 8.46±3.45 MPa and 7.7±2.24 MPa for the HLT and FSLT, respectively; the difference was not statistically significant (P=0.46). Conclusions The layering technique by which composite resin was packed into the root canal of primary anterior teeth (HLT versus FSLT) had no significant effect on the push-out bond strength of composite resin posts.", "gemini": "Mục tiêu Nghiên cứu trong ống nghiệm này nhằm so sánh độ bền liên kết đẩy ra của chốt nhựa composite được đóng vào ống tủy của răng cửa trước tiên phát bằng hai kỹ thuật xếp lớp khác nhau. Vật liệu và Phương pháp Ba mươi hai răng cửa trước tiên phát được chia ngẫu nhiên thành hai nhóm. Ở nhóm 1, sau khi chuẩn bị khoảng trống cho chốt, nhựa composite sau (Filtek P60) được đóng thành ba lớp nằm ngang bằng dụng cụ nén composite có đầu hình trụ sử dụng kỹ thuật xếp lớp nằm ngang (HLT). Ở nhóm 2, thực hiện bằng dụng cụ nén có đầu hình nón thành ba lớp hình phễu theo kỹ thuật xếp lớp hình phễu (FSLT). Tiếp theo, các mẫu vật được thử độ bền liên kết đẩy ra. Dữ liệu được phân tích bằng kiểm định t và đường cong Kaplan-Meier. Kết quả Độ bền liên kết trung bình ± độ lệch chuẩn (SD) của chốt nhựa composite là 8,46 ± 3,45 MPa và 7,7 ± 2,24 MPa đối với HLT và FSLT; sự khác biệt không có ý nghĩa thống kê (P = 0,46). Kết luận Kỹ thuật xếp lớp đóng nhựa composite vào ống tủy của răng cửa trước tiên phát (HLT so với FSLT) không có tác động đáng kể đến độ bền liên kết đẩy ra của chốt nhựa composite."} +{"idx": 1910068, "en": "Objectives Considering the optimal efficacy of bromelain for pain relief and wound healing, this study aimed to assess the effect of bromelain on wound healing, pain, and bleeding at the donor site following free gingival grafting (FGG). Materials and Methods This randomized, controlled double-blind clinical trial was performed on 26 patients with gingival recession. The patients were randomly divided into two groups of bromelain and placebo (n=13). Treatment was started on the day of surgery and was continued for 10 days. Pain, bleeding, and epithelialization at the donor site were the variables evaluated in this study using a questionnaire. The level of pain was determined using a visual analog scale (VAS) considering the number of analgesic tablets taken within 7 days postoperatively. Bleeding was determined according to the patient's report, and epithelization was assessed by applying 3% hydrogen peroxide (H2O2) to the donor site. The donor site epithelialization was assessed at 7 and 10 days after surgery. Results Bromelain caused a significant reduction in pain at the donor site (2.605±0.509) compared to the placebo (4.885±0.519; P<0.05). The number of donor sites with complete epithelialization was higher in the bromelain group compared to the placebo, but this difference was not statistically significant (P>0.05). The two groups were the same regarding postoperative bleeding (P>0.05). Conclusions The results showed that oral bromelain (500 mg/day) can be effective in the reduction of pain at the donor site after FGG and may also enhance wound healing. Oral bromelain does not increase the risk of postoperative bleeding.", "gemini": "Mục tiêu Xem xét hiệu quả tối ưu của bromelain trong việc giảm đau và lành vết thương, nghiên cứu này nhằm đánh giá tác dụng của bromelain đối với quá trình lành vết thương, đau và chảy máu tại vị trí lấy ghép sau khi ghép nướu tự do (FGG). Vật liệu và Phương pháp Thử nghiệm lâm sàng mù đôi có đối chứng ngẫu nhiên này được thực hiện trên 26 bệnh nhân bị tụt nướu. Những bệnh nhân này được chia ngẫu nhiên thành hai nhóm bromelain và giả dược (n = 13). Điều trị bắt đầu vào ngày phẫu thuật và tiếp tục trong 10 ngày. Đau, chảy máu và biểu mô hóa tại vị trí lấy ghép là các biến được đánh giá trong nghiên cứu này bằng cách sử dụng bảng câu hỏi. Mức độ đau được xác định bằng thang tương tự thị giác (VAS) xem xét số lượng viên thuốc giảm đau được dùng trong vòng 7 ngày sau phẫu thuật. Chảy máu được xác định theo báo cáo của bệnh nhân và biểu mô hóa được đánh giá bằng cách bôi 3% hydro peroxide (H2O2) vào vị trí lấy ghép. Biểu mô hóa tại vị trí lấy ghép được đánh giá sau 7 và 10 ngày sau phẫu thuật. Kết quả Bromelain làm giảm đáng kể cơn đau tại vị trí lấy ghép (2,605 ± 0,509) so với giả dược (4,885 ± 0,519; P < 0,05). Số vị trí lấy ghép biểu mô hóa hoàn toàn cao hơn ở nhóm bromelain so với giả dược, nhưng sự khác biệt này không có ý nghĩa thống kê (P > 0,05). Hai nhóm giống nhau về chảy máu sau phẫu thuật (P > 0,05). Kết luận Kết quả cho thấy bromelain đường uống (500 mg/ngày) có thể có hiệu quả trong việc giảm đau tại vị trí lấy ghép sau FGG và cũng có thể thúc đẩy quá trình lành vết thương. Bromelain đường uống không làm tăng nguy cơ chảy máu sau phẫu thuật."} +{"idx": 1910069, "en": "Objectives This study aimed to evaluate the effect of oral melatonin and oral midazolam as premedication for intravenous (IV) sedation of pediatric dental patients. Materials and Methods This crossover, double-blind randomized clinical trial was conducted on 23 uncooperative 2-6-year-olds with definitely negative behaviors according to the Frankl's scale. Each child served as their own control. The children were randomly divided into two groups: group I received 0.5mg/kg of oral melatonin one hour before IV sedation, while group II received 0.5mg/kg of oral midazolam 30 minutes before IV sedation on their first visit. Every child received the other premedication on their second visit. The degree of sedation was judged according to the Houpt scale. Physiologic parameters including blood pressure (PB), heart rate (HR), and blood oxygen saturation (SpO2) and side effects including dizziness, nausea, vomiting, and sleepiness were assessed. The parents' and the operator's satisfaction rates were scored. Data were analyzed using paired t-test and Wilcoxon signed-rank test. Results There were significant differences in sedation scores between the two sessions (P<0.05). However, there were no significant differences in alterations of physiologic parameters between the two sessions (P>0.05). Nausea and vomiting were more common during the first two hours in the midazolam group (P=0.002). Tremors were more common in the melatonin group (P=0.013). Dizziness was more evident when melatonin was used (P<0.001). The clinician and the parents were more satisfied with the results of midazolam intake (P<0.05). Conclusions Premedication with oral midazolam in pediatric patients is superior to that with melatonin with a higher parents' and operator's satisfaction.", "gemini": "Mục tiêu Nghiên cứu này nhằm đánh giá hiệu quả của melatonin uống và midazolam uống như là thuốc tiền mê cho việc gây mê tĩnh mạch (IV) ở bệnh nhi nha khoa. Vật liệu và phương pháp Thử nghiệm lâm sàng ngẫu nhiên, mù đôi, chéo này được tiến hành trên 23 trẻ em không hợp tác từ 2-6 tuổi có hành vi tiêu cực rõ ràng theo thang điểm của Frankl. Mỗi trẻ là đối chứng của chính mình. Trẻ em được chia ngẫu nhiên thành hai nhóm: nhóm I dùng 0,5mg/kg melatonin uống một giờ trước khi gây mê IV, trong khi nhóm II dùng 0,5mg/kg midazolam uống 30 phút trước khi gây mê IV trong lần khám đầu tiên. Mỗi trẻ đều dùng thuốc tiền mê còn lại trong lần khám thứ hai. Mức độ an thần được đánh giá theo thang điểm Houpt. Các thông số sinh lý bao gồm huyết áp (PB), nhịp tim (HR) và độ bão hòa oxy trong máu (SpO2) và các tác dụng phụ bao gồm chóng mặt, buồn nôn, nôn và buồn ngủ đã được đánh giá. Tỷ lệ hài lòng của cha mẹ và bác sĩ phẫu thuật đã được ghi điểm. Dữ liệu được phân tích bằng kiểm định t ghép đôi và kiểm định Wilcoxon signed-rank. Kết quả Có sự khác biệt đáng kể về điểm an thần giữa hai lần (P < 0,05). Tuy nhiên, không có sự khác biệt đáng kể nào về sự thay đổi các thông số sinh lý giữa hai lần (P> 0,05). Buồn nôn và nôn phổ biến hơn trong hai giờ đầu ở nhóm midazolam (P = 0,002). Run rẩy phổ biến hơn ở nhóm melatonin (P = 0,013). Chóng mặt rõ ràng hơn khi sử dụng melatonin (P < 0,001). Bác sĩ lâm sàng và cha mẹ hài lòng hơn với kết quả dùng midazolam (P < 0,05). Kết luận Thuốc tiền mê midazolam uống ở bệnh nhi vượt trội hơn melatonin với sự hài lòng cao hơn của cha mẹ và bác sĩ phẫu thuật."} +{"idx": 1910070, "en": "Objectives Pressure transmission under denture bases can vary depending on the denture tooth material. The aim of the present study was to evaluate pressure transmission under denture bases using denture teeth of different materials in direct and indirect tooth contacts. Materials and Methods In this in-vitro study, the pressure transmission generated by five types of denture teeth, including ceramic, nanocomposite, composite-acrylic resin, cross-linked acrylic resin, and polymethyl methacrylate (PMMA), under direct and indirect pressures was evaluated (n=10). The maximum pressure (MPa) was measured using a strain gauge. Data were statistically analyzed with one-way analysis of variance (ANOVA; α=0.05). Results The denture tooth material had a significant effect on pressure transmission under denture bases (P<0.001). Under direct load, ceramic and PMMA teeth exhibited the maximum and minimum pressures, respectively, contrary to indirect load (P<0.001). Conclusions Pressure transmission under denture bases significantly varies with the use of different denture tooth materials. Acrylic teeth could be the most favorable choice to reduce the pressure beneath denture bases. Nanocomposite and acrylic resin-composite teeth may be used as alternatives.", "gemini": "Mục tiêu Áp lực truyền dưới nền hàm giả có thể thay đổi tùy thuộc vào vật liệu răng hàm giả. Mục đích của nghiên cứu hiện tại là đánh giá áp lực truyền dưới nền hàm giả bằng cách sử dụng răng hàm giả làm bằng các vật liệu khác nhau khi tiếp xúc răng trực tiếp và gián tiếp. Vật liệu và phương pháp Trong nghiên cứu trong ống nghiệm này, áp lực truyền do năm loại răng hàm giả tạo ra, bao gồm gốm, nanocomposite, nhựa acrylic composite, nhựa acrylic liên kết chéo và polymethyl methacrylate (PMMA), dưới áp lực trực tiếp và gián tiếp đã được đánh giá (n = 10). Áp suất tối đa (MPa) được đo bằng máy đo ứng suất. Dữ liệu được phân tích thống kê bằng phân tích phương sai một chiều (ANOVA; α = 0,05). Kết quả Vật liệu răng hàm giả có ảnh hưởng đáng kể đến áp lực truyền dưới nền hàm giả (P < 0,001). Dưới tải trọng trực tiếp, răng gốm và PMMA thể hiện áp lực tối đa và tối thiểu, ngược lại với tải trọng gián tiếp (P < 0,001). Kết luận Áp lực truyền dưới nền hàm giả thay đổi đáng kể khi sử dụng các vật liệu răng hàm giả khác nhau. Răng acrylic có thể là lựa chọn thuận lợi nhất để giảm áp lực bên dưới nền hàm giả. Răng nanocomposite và răng composite-nhựa acrylic có thể được sử dụng làm phương án thay thế."} +{"idx": 1910071, "en": "Background Innate immunity utilizes components of sensory signal transduction such as bitter and sweet taste receptors. In fact, empirical evidence has shown bitter and sweet taste receptors to be an integral component of antimicrobial immune response in upper respiratory tract infections. Since an efficient immune response plays a key role in the attainment of longevity, it is not surprising that the rs978739 polymorphism of the bitter taste receptor TAS2R16 gene has been shown to be associated with longevity in a population of 941 individuals ranging in age from 20 to 106 years from Calabria (Italy). There are many possible candidate genes for human longevity, however of the many genes tested, only APOE and FOXO3 survived to association in replication studies. So, it is necessary to validate in other studies genes proposed to be associated with longevity. Thus, we analysed the association of the quoted polymorphism in a population of long lived individuals (LLIs) and controls from another Italian population from Cilento. Methods The analysis has been performed on data previously obtained with genome-wide association study on a population of LLIs (age range 90-109 years) and young controls (age range 18-45 years) from Cilento (Italy). Results Statistical power calculations showed that the analysed cohort represented by 410 LLIs and 553 young controls was sufficiently powered to replicate the association between rs978739 and the longevity phenotype according to the effect size and frequencies described in the previous paper, under a dominant and additive genetic model. However, no evidence of association between rs978739 and the longevity phenotype was observed according to the additive or dominant model. Conclusion There are several reasons for the failure of the confirmation of a previous study. However, the differences between the two studies in terms of environment of the population adopted and of the criteria of inclusion have made difficult the replication of the findings.", "gemini": "Lý thuyết Miễn dịch bẩm sinh sử dụng các thành phần của quá trình chuyển tín hiệu cảm giác như thụ thể vị đắng và ngọt. Trên thực tế, bằng chứng thực nghiệm đã chỉ ra rằng thụ thể vị đắng và ngọt là một thành phần không thể thiếu của phản ứng miễn dịch chống vi khuẩn trong các bệnh nhiễm trùng đường hô hấp trên. Vì một phản ứng miễn dịch hiệu quả đóng vai trò quan trọng trong việc đạt được tuổi thọ, nên không có gì ngạc nhiên khi đa hình rs978739 của gen thụ thể vị đắng TAS2R16 đã được chứng minh là có liên quan đến tuổi thọ trong quần thể gồm 941 cá thể có độ tuổi từ 20 đến 106 tuổi từ Calabria (Ý). Có nhiều gen ứng viên có thể có liên quan đến tuổi thọ của con người, tuy nhiên trong số nhiều gen đã thử nghiệm, chỉ có APOE và FOXO3 sống sót để liên kết trong các nghiên cứu nhân bản. Vì vậy, cần phải xác nhận trong các nghiên cứu khác về các gen được đề xuất là có liên quan đến tuổi thọ. Do đó, chúng tôi đã phân tích mối liên quan của đa hình được trích dẫn trong một quần thể những cá nhân sống lâu (LLI) và đối chứng từ một quần thể khác của Ý từ Cilento. Phương pháp Phân tích đã được thực hiện trên dữ liệu thu được trước đó bằng nghiên cứu liên kết toàn bộ hệ gen trên quần thể LLI (khoảng tuổi 90-109 tuổi) và đối chứng trẻ (khoảng tuổi 18-45 tuổi) từ Cilento (Ý). Kết quả Các tính toán về sức mạnh thống kê cho thấy nhóm nghiên cứu được phân tích đại diện bởi 410 LLI và 553 đối chứng trẻ đủ khả năng để nhân bản mối liên quan giữa rs978739 và kiểu hình tuổi thọ theo kích thước hiệu ứng và tần số được mô tả trong bài báo trước, theo mô hình di truyền trội và cộng gộp. Tuy nhiên, không có bằng chứng nào về mối liên quan giữa rs978739 và kiểu hình tuổi thọ được quan sát theo mô hình cộng gộp hoặc trội. Kết luận Có một số lý do khiến việc xác nhận một nghiên cứu trước đó không thành công. Tuy nhiên, sự khác biệt giữa hai nghiên cứu về môi trường của quần thể được áp dụng và tiêu chuẩn tuyển chọn đã gây khó khăn cho việc nhân rộng các phát hiện."} +{"idx": 1910072, "en": "Background When taking photographs of plants in the field, it is often necessary to record additional information such as sample number, biological replicate number and subspecies. Manual methods of recording such information are slow, often involve laborious transcription from hand-written notes or the need to have a laptop or tablet on site, and present a risk by separating written data capture from image capture. Existing tools for field data capture focus on recording information rather than capturing pictures of plants. Results We present SpotCard, a tool comprising two macros. The first can be used to create a template for small, reusable cards for use when photographing plants. Information can be encoded on these cards in a human- and machine-readable form, allowing the user to swiftly make annotations before taking the photograph. The second part of the tool automatically reads the annotations from the image and tabulates them in a CSV file, along with picture date, time and GPS coordinates. The SpotCard also provides a convenient scale bar and coordinate location within the image for the flower itself, enabling automated measurement of floral traits such as area and perimeter. Conclusions This tool is shown to read annotations with a high degree of accuracy and at a speed greatly faster than manual transcription. It includes the ability to read the date and time of the photograph, as well as GPS location. It is an open-source ImageJ/Fiji macro and is available online. Its use requires no knowledge of the ImageJ macro coding language, and it is therefore well suited to all researchers taking pictures in the field.", "gemini": "Bối cảnh Khi chụp ảnh thực vật ngoài thực địa, thường cần ghi lại thông tin bổ sung như số mẫu, số bản sao sinh học và phân loài. Các phương pháp ghi thủ công thông tin như vậy rất chậm, thường liên quan đến việc sao chép công phu từ các ghi chú viết tay hoặc cần có máy tính xách tay hoặc máy tính bảng tại chỗ và có nguy cơ do việc chụp dữ liệu bằng văn bản tách biệt với việc chụp ảnh. Các công cụ hiện có để thu thập dữ liệu ngoài thực địa tập trung vào việc ghi thông tin thay vì chụp ảnh thực vật. Kết quả Chúng tôi trình bày SpotCard, một công cụ gồm hai macro. Đầu tiên có thể được sử dụng để tạo mẫu cho các thẻ nhỏ, có thể tái sử dụng để sử dụng khi chụp ảnh thực vật. Thông tin có thể được mã hóa trên các thẻ này ở dạng có thể đọc được bằng máy và con người, cho phép người dùng nhanh chóng tạo chú thích trước khi chụp ảnh. Phần thứ hai của công cụ tự động đọc các chú thích từ hình ảnh và lập bảng chúng trong tệp CSV, cùng với ngày, giờ chụp ảnh và tọa độ GPS. SpotCard cũng cung cấp một thanh tỷ lệ thuận tiện và vị trí tọa độ trong hình ảnh cho chính bông hoa, cho phép đo tự động các đặc điểm của hoa như diện tích và chu vi. Kết luận Công cụ này cho thấy khả năng đọc chú thích với độ chính xác cao và tốc độ nhanh hơn nhiều so với phiên âm thủ công. Nó bao gồm khả năng đọc ngày và giờ chụp ảnh, cũng như vị trí GPS. Đây là macro ImageJ/Fiji nguồn mở và có sẵn trực tuyến. Việc sử dụng nó không yêu cầu kiến thức về ngôn ngữ lập trình macro ImageJ, do đó, nó phù hợp với tất cả các nhà nghiên cứu chụp ảnh ngoài thực địa."} +{"idx": 1910073, "en": "Background Fed-batch conditions are advantageous for industrial cultivations as they avoid unfavorable phenomena appearing in batch cultivations. Those are for example the formation of overflow metabolites, catabolite repression, oxygen limitation or inhibition due to elevated osmotic concentrations. For both, the early bioprocess development and the optimization of existing bioprocesses, small-scale reaction vessels are applied to ensure high throughput, low costs and prompt results. However, most conventional small-scale procedures work in batch operation mode, which stands in contrast to fed-batch conditions in large-scale bioprocesses. Extensive expenditure for installations and operation accompany almost all cultivation systems in the market allowing fed-batch conditions in small-scale. An alternative, more cost efficient enzymatic glucose release system is strongly influenced by environmental conditions. To overcome these issues, this study investigates a polymer-based fed-batch system for controlled substrate release in microtiter plates. Results Immobilizing a solid silicone matrix with embedded glucose crystals at the bottom of each well of a microtiter plate is a suitable technique for implementing fed-batch conditions in microtiter plates. The results showed that the glucose release rate depends on the osmotic concentration, the pH and the temperature of the medium. Moreover, the applied nitrogen source proved to influence the glucose release rate. A new developed mathematical tool predicts the glucose release for various media conditions. The two model organisms E. coli and H. polymorpha were cultivated in the fed-batch microtiter plate to investigate the general applicability for microbial systems. Online monitoring of the oxygen transfer rate and offline analysis of substrate, product, biomass and pH confirmed that fed-batch conditions are comparable to large-scale cultivations. Furthermore, due to fed-batch conditions in microtiter plates, product formation could be enhanced by the factor 245 compared to batch cultivations. Conclusions The polymer-based fed-batch microtiter plate represents a sophisticated and cost efficient system to mimic typical industrial fed-batch conditions in small-scale. Thus, a more reliable strain screening and early process development can be performed. A systematical scale-down with low expenditure of work, time and money is possible.", "gemini": "Bối cảnh Điều kiện nuôi cấy theo từng đợt có lợi cho việc nuôi cấy công nghiệp vì chúng tránh được các hiện tượng bất lợi xuất hiện trong nuôi cấy theo mẻ. Ví dụ như sự hình thành các chất chuyển hóa tràn, sự ức chế dị hóa, sự hạn chế oxy hoặc sự ức chế do nồng độ thẩm thấu tăng cao. Đối với cả quá trình phát triển sinh học ban đầu và tối ưu hóa các quy trình sinh học hiện có, các bình phản ứng quy mô nhỏ được áp dụng để đảm bảo thông lượng cao, chi phí thấp và kết quả nhanh chóng. Tuy nhiên, hầu hết các quy trình quy mô nhỏ thông thường hoạt động ở chế độ vận hành theo mẻ, trái ngược với các điều kiện nuôi cấy theo từng đợt trong các quy trình sinh học quy mô lớn. Chi phí lớn cho các cơ sở lắp đặt và vận hành đi kèm với hầu hết mọi hệ thống nuôi cấy trên thị trường cho phép các điều kiện nuôi cấy theo từng đợt ở quy mô nhỏ. Một hệ thống giải phóng glucose bằng enzym thay thế, hiệu quả hơn về chi phí bị ảnh hưởng mạnh bởi các điều kiện môi trường. Để khắc phục những vấn đề này, nghiên cứu này điều tra một hệ thống nuôi cấy theo từng đợt dựa trên polyme để giải phóng chất nền được kiểm soát trong các đĩa vi lượng. Kết quả Việc cố định một ma trận silicon rắn với các tinh thể glucose nhúng ở dưới cùng của mỗi giếng của đĩa vi lượng là một kỹ thuật phù hợp để thực hiện các điều kiện nuôi cấy theo từng đợt trong các đĩa vi lượng. Kết quả cho thấy tốc độ giải phóng glucose phụ thuộc vào nồng độ thẩm thấu, độ pH và nhiệt độ của môi trường. Hơn nữa, nguồn nitơ được áp dụng đã được chứng minh là ảnh hưởng đến tốc độ giải phóng glucose. Một công cụ toán học mới được phát triển dự đoán sự giải phóng glucose cho các điều kiện môi trường khác nhau. Hai sinh vật mô hình E. coli và H. polymorpha được nuôi cấy trong đĩa vi lượng nuôi cấy theo từng đợt để nghiên cứu khả năng áp dụng chung cho các hệ thống vi sinh vật. Việc theo dõi trực tuyến tốc độ truyền oxy và phân tích ngoại tuyến về chất nền, sản phẩm, sinh khối và độ pH đã xác nhận rằng các điều kiện nuôi cấy theo từng đợt có thể so sánh được với các quy trình nuôi cấy quy mô lớn. Hơn nữa, do các điều kiện nuôi cấy theo từng đợt trong các đĩa vi lượng, quá trình hình thành sản phẩm có thể được tăng cường gấp 245 lần so với nuôi cấy theo mẻ. Kết luận Đĩa vi lượng nuôi cấy theo từng đợt dựa trên polyme là một hệ thống tinh vi và hiệu quả về chi phí để mô phỏng các điều kiện nuôi cấy theo từng đợt công nghiệp thông thường ở quy mô nhỏ. Do đó, có thể thực hiện sàng lọc chủng tin cậy hơn và phát triển quy trình sớm hơn. Có thể thu nhỏ quy mô một cách có hệ thống với chi phí công sức, thời gian và tiền bạc thấp."} +{"idx": 1910074, "en": "Background This study evaluated the individual and combined diagnostic performance of the bacterial artificial chromosomes (BACs)-on-Beads (BoBs™) assay and conventional karyotyping for the prenatal detection of chromosomal abnormalities in pregnant women who were 35 or more years-old. Method The primary outcome was concordance of any numerical, structural, or submicroscopic chromosomal abnormalities between BoBs™ and conventional karyotyping of amniotic fluid specimens from pregnant women at 17 to 22 weeks gestation. Results We examined samples from 4852 pregnant women. BoBs™ indicated that 4708 samples were normal (97.03%), and 144 were abnormal (2.97%); conventional karyotyping indicated that 4656 (95.96%) samples were normal and 196 (4.04%) were abnormal. The combined use of both methods indicated that 4633 of 4852 samples were normal (95.49%) and 219 of 4852 samples (4.51%) were abnormal. The kappa coefficient of the combined test was 0.70, indicating substantial consistency between BoBs™ and conventional karyotyping (95% CI = 0.65-0.76, P < 0.001). Conclusions Our results indicate that the combined use of BoBs™ and conventional karyotyping detected more fetal abnormalities than either test alone.", "gemini": "Bối cảnh Nghiên cứu này đánh giá hiệu suất chẩn đoán riêng lẻ và kết hợp của xét nghiệm nhiễm sắc thể nhân tạo của vi khuẩn (BAC)-trên-Hạt (BoBs™) và kiểu nhân truyền thống để phát hiện trước khi sinh các bất thường về nhiễm sắc thể ở phụ nữ mang thai từ 35 tuổi trở lên. Phương pháp Kết quả chính là sự phù hợp của bất kỳ bất thường về nhiễm sắc thể về số lượng, cấu trúc hoặc dưới vi mô nào giữa BoBs™ và kiểu nhân truyền thống của các mẫu dịch ối từ phụ nữ mang thai từ tuần 17 đến 22. Kết quả Chúng tôi đã kiểm tra các mẫu từ 4852 phụ nữ mang thai. BoBs™ chỉ ra rằng 4708 mẫu là bình thường (97,03%) và 144 mẫu bất thường (2,97%); kiểu nhân truyền thống chỉ ra rằng 4656 (95,96%) mẫu là bình thường và 196 (4,04%) mẫu bất thường. Việc sử dụng kết hợp cả hai phương pháp chỉ ra rằng 4633 trong số 4852 mẫu là bình thường (95,49%) và 219 trong số 4852 mẫu (4,51%) là bất thường. Hệ số kappa của xét nghiệm kết hợp là 0,70, cho thấy sự nhất quán đáng kể giữa BoBs™ và kiểu nhân truyền thống (95% CI = 0,65-0,76, P < 0,001). Kết luận Kết quả của chúng tôi chỉ ra rằng việc sử dụng kết hợp BoBs™ và kiểu nhân truyền thống phát hiện ra nhiều bất thường ở thai nhi hơn so với chỉ một xét nghiệm."} +{"idx": 1910075, "en": "Background The loss of response to adalimumab (ADL) has been related to low serum concentrations at trough. Currently, most methods commercially available for the quantification of ADL are enzyme-linked immunosorbent assay (ELISA) based, with a turnaround time of approximately 8 h, delaying the target dosage adjustment to the subsequent infusion. In this study, we aimed to evaluate the performance of the newly available rapid-test ADL quantification assay by comparing it with three established ELISA methods, using spiked samples and a set of clinical samples. Methods Spiked samples from control donors and 120 serum samples from inflammatory bowel disease (IBD) patients undergoing ADL therapy were quantified using lateral flow Quantum Blue® Adalimumab and, the ELISA formats from Immundiagnostik, R-Biopharm and an in-house assay. Results The rapid-test assay had intraclass correlation coefficients of 0.590, 0.864 and 0.761 when comparing with the Immundiagnostik, R-Biopharm and in-house assays, respectively. For the five therapeutic windows, the accuracy was high: ADL rapid test compared with the Immundiagnostik (58-88%); R-Biopharm, 68-89%; and in house, 60-88%; and kappa statistics revealed 0.492-0.602, 0.531-0.659 and 0.545-0.682, respectively. Conclusions The Quantum Blue® Adalimumab assay can replace the commonly used ELISA-based ADL quantification kits and it is a reliable alternative to these methods. This rapid-test assay enables the quantitative determination of ADL serum trough level in only 15 min. The developed assay allows measurement of ADL over a wide range. Hence, it represents a valuable tool for the clinician to assess the ADL trough level.", "gemini": "Bối cảnh Việc mất đáp ứng với adalimumab (ADL) có liên quan đến nồng độ huyết thanh thấp ở đáy. Hiện nay, hầu hết các phương pháp định lượng ADL có sẵn trên thị trường đều dựa trên xét nghiệm miễn dịch liên kết với enzyme (ELISA), với thời gian xử lý khoảng 8 giờ, làm chậm quá trình điều chỉnh liều mục tiêu cho lần truyền tiếp theo. Trong nghiên cứu này, chúng tôi nhằm mục đích đánh giá hiệu suất của xét nghiệm định lượng ADL nhanh mới có sẵn bằng cách so sánh nó với ba phương pháp ELISA đã được thiết lập, sử dụng các mẫu có pha tạp và một bộ mẫu lâm sàng. Phương pháp Các mẫu có pha tạp từ những người hiến tặng đối chứng và 120 mẫu huyết thanh từ những bệnh nhân mắc bệnh viêm ruột (IBD) đang điều trị bằng ADL đã được định lượng bằng phương pháp dòng chảy bên Quantum Blue® Adalimumab và các định dạng ELISA từ Immundiagnostik, R-Biopharm và một xét nghiệm nội bộ. Kết quả Xét nghiệm nhanh có hệ số tương quan nội nhóm là 0,590, 0,864 và 0,761 khi so sánh với các xét nghiệm Immundiagnostik, R-Biopharm và nội bộ, tương ứng. Đối với năm cửa sổ điều trị, độ chính xác cao: xét nghiệm nhanh ADL so với Immundiagnostik (58-88%); R-Biopharm, 68-89%; và nội bộ, 60-88%; và số liệu thống kê kappa lần lượt là 0,492-0,602, 0,531-0,659 và 0,545-0,682. Kết luận Xét nghiệm Quantum Blue® Adalimumab có thể thay thế các bộ dụng cụ định lượng ADL dựa trên ELISA thường dùng và đây là phương pháp thay thế đáng tin cậy cho các phương pháp này. Xét nghiệm nhanh này cho phép xác định định lượng nồng độ đáy huyết thanh ADL chỉ trong 15 phút. Xét nghiệm được phát triển cho phép đo ADL trong một phạm vi rộng. Do đó, đây là một công cụ có giá trị cho bác sĩ lâm sàng để đánh giá nồng độ đáy ADL."} +{"idx": 1910076, "en": "Clinical trials have advanced the treatment of multiple sclerosis (MS) by demonstrating the safety and efficacy of disease-modifying therapies (DMTs). This review discusses major changes to MS clinical trials in the era of DMTs. As treatment options for MS continue to increase, patients in modern MS trials present earlier and with milder disease compared with historic MS populations. While placebo-controlled trials for some questions may still be relevant, DMT trials in relapsing-remitting MS (RRMS) are no longer ethical. The replacement of the placebo arm by an active comparator arm in trials have raised the cost of trials by requiring larger sample sizes to detect on-study changes in treatment effects. Efforts to improve trial efficiency in RRMS have focused on exploring adaptive designs and relying on sensitive magnetic resonance imaging measures of disease activity. In trials for progressive forms of MS (PMS), the lack of sensitive outcome measures that can be used in shorter-term trials have delayed the development of effective treatments. Recent shifting of the focus to advancing trials in PMS has identified paraclinical outcome measurements with improved potential, and the testing of agents for neuroprotection and remyelination is in progress.", "gemini": "Các thử nghiệm lâm sàng đã thúc đẩy việc điều trị bệnh đa xơ cứng (MS) bằng cách chứng minh tính an toàn và hiệu quả của các liệu pháp điều chỉnh bệnh (DMT). Đánh giá này thảo luận về những thay đổi chính đối với các thử nghiệm lâm sàng về MS trong kỷ nguyên của DMT. Khi các lựa chọn điều trị cho MS tiếp tục tăng, bệnh nhân trong các thử nghiệm MS hiện đại xuất hiện sớm hơn và mắc bệnh nhẹ hơn so với các quần thể MS trong lịch sử. Mặc dù các thử nghiệm có đối chứng giả dược đối với một số câu hỏi vẫn có thể có liên quan, nhưng các thử nghiệm DMT ở bệnh MS tái phát-giảm nhẹ (RRMS) không còn mang tính đạo đức nữa. Việc thay thế nhóm giả dược bằng nhóm so sánh tích cực trong các thử nghiệm đã làm tăng chi phí của các thử nghiệm bằng cách yêu cầu kích thước mẫu lớn hơn để phát hiện những thay đổi trong hiệu quả điều trị khi nghiên cứu. Những nỗ lực cải thiện hiệu quả thử nghiệm ở RRMS tập trung vào việc khám phá các thiết kế thích ứng và dựa vào các biện pháp cộng hưởng từ nhạy cảm để đo hoạt động của bệnh. Trong các thử nghiệm đối với các dạng tiến triển của MS (PMS), việc thiếu các biện pháp kết quả nhạy có thể được sử dụng trong các thử nghiệm ngắn hạn đã trì hoãn việc phát triển các phương pháp điều trị hiệu quả. Sự thay đổi gần đây trong trọng tâm để thúc đẩy các thử nghiệm ở PMS đã xác định các phép đo kết quả cận lâm sàng với tiềm năng được cải thiện, và việc thử nghiệm các tác nhân bảo vệ thần kinh và tái tạo myelin đang được tiến hành."} +{"idx": 1910077, "en": "Background Large-vessel atherosclerotic disease is an important pathogenesis of deep-perforator infarction (DPI). However, altered vessel walls of intracranial large arteries and distribution of small arteries in DPI are unclear because of the limited resolution of current imaging techniques. In this study the intracranial plaque burden and lenticulostriate artery (LSA) distribution in patients with recent DPI and non-DPI using whole-brain vessel-wall imaging (WB-VWI) were investigated. Methods A total of 44 patients with recent DPI (23 patients) or non-DPI (21 patients) due to intracranial atherosclerotic disease were prospectively enrolled. WB-VWI was performed in all the patients using a three-dimensional T1-weighted vessel-wall magnetic resonance technique. Hemispheres with DPI and non-DPI were considered as the DPI group and non-DPI group, respectively. Hemispheres without a history of stroke were the control group. The intracranial plaque burden was compared between the DPI and non-DPI groups. The number and length of visualized LSA branches among DPI, non-DPI, and control groups were also evaluated. Results A total of 77 hemispheres were analyzed (23 in the DPI group, 21 in the non-DPI group, and 33 in the control group). Plaque burden was lower (p = 0.047) in the DPI group (82.0 ± 45.9 mm3) compared with the non-DPI group (130.9 ± 90.3 mm3). There was a significant reduction (p = 0.002) in length of visualized LSA branches in the DPI group (74.1 ± 21.7 mm) compared with the control group (104.6 ± 33.3 mm). Conclusions WB-VWI enables the combination of vessel-wall and LSA imaging in one image setting, which can provide information about plaque burden and LSA distribution.", "gemini": "Bối cảnh Bệnh xơ vữa động mạch mạch lớn là một cơ chế sinh bệnh quan trọng của nhồi máu lỗ sâu (DPI). Tuy nhiên, sự thay đổi của thành mạch ở các động mạch lớn trong sọ và sự phân bố của các động mạch nhỏ ở DPI vẫn chưa rõ ràng do độ phân giải hạn chế của các kỹ thuật hình ảnh hiện tại. Trong nghiên cứu này, gánh nặng mảng bám trong sọ và sự phân bố của động mạch thấu kính (LSA) ở những bệnh nhân bị DPI gần đây và không bị DPI bằng cách sử dụng hình ảnh thành mạch toàn não (WB-VWI) đã được nghiên cứu. Phương pháp Tổng cộng 44 bệnh nhân bị DPI gần đây (23 bệnh nhân) hoặc không bị DPI (21 bệnh nhân) do bệnh xơ vữa động mạch trong sọ đã được tuyển dụng. WB-VWI được thực hiện ở tất cả bệnh nhân bằng kỹ thuật cộng hưởng từ thành mạch có trọng số T1 ba chiều. Các bán cầu có DPI và không có DPI được coi tương ứng là nhóm DPI và nhóm không có DPI. Các bán cầu không có tiền sử đột quỵ là nhóm đối chứng. Gánh nặng mảng bám trong sọ được so sánh giữa nhóm DPI và nhóm không có DPI. Số lượng và chiều dài các nhánh LSA được hình dung giữa các nhóm DPI, không có DPI và nhóm đối chứng cũng được đánh giá. Kết quả Tổng cộng 77 bán cầu đã được phân tích (23 trong nhóm DPI, 21 trong nhóm không có DPI và 33 trong nhóm đối chứng). Gánh nặng mảng bám thấp hơn (p = 0,047) ở nhóm DPI (82,0 ± 45,9 mm3) so với nhóm không có DPI (130,9 ± 90,3 mm3). Có sự giảm đáng kể (p = 0,002) về chiều dài các nhánh LSA được hình dung ở nhóm DPI (74,1 ± 21,7 mm) so với nhóm đối chứng (104,6 ± 33,3 mm). Kết luận WB-VWI cho phép kết hợp hình ảnh thành mạch và LSA trong một cài đặt hình ảnh, có thể cung cấp thông tin về gánh nặng mảng bám và sự phân bố LSA."} +{"idx": 1910078, "en": "Background Patients with type 2 diabetes have an increased risk of cognitive impairment which can lead to impaired diabetes self-management and an increased risk of diabetes-related complications. Routine screening for cognitive impairment in elderly patients with type 2 diabetes is therefore increasingly advocated. The aim of this study is to investigate whether people with type 2 diabetes and screen-detected cognitive impairment use acute health care services more often than patients not suspected of cognitive impairment. Methods People with type 2 diabetes ≥ 70 years were screened for cognitive impairment in primary care. Diagnoses in screen positives were established at a memory clinic. Information about acute health care use was collected for 2 years prior to and 2 years after screening and compared to screen negatives. Results 154 participants (38% female, mean age 76.7 ± 5.2 years, diabetes duration 8.7 ± 8.2 years) were included, 37 patients with cognitive impairment, 117 screen negatives. A higher percentage of participants with cognitive impairment compared to screen negative patients used acute health care services; this difference was significant for general practitioner's out of hours services (56% versus 34% used this service over 4 years, p = 0.02). The mean number of acute health care visits was also higher in those with cognitive impairment than in screen negatives (2.2 ± 2.8 versus 1.4 ± 2.2 visits in 4 years, p < 0.05; 1.4 ± 2.2 versus 0.7 ± 1.5 visits in 2 years after screening, p = 0.03). Factors that could have played a role in this increased risk of acute health care services use were a low educational level, the presence of depressive symptoms (CES-D score ≥ 16), self-reported problems in self-care and self-reported problems in usual activities. Conclusions People with type 2 diabetes and screen-detected cognitive impairment use acute health care services more often.", "gemini": "Bối cảnh Bệnh nhân mắc bệnh đái tháo đường týp 2 có nguy cơ suy giảm nhận thức cao hơn, có thể dẫn đến việc tự quản lý bệnh đái tháo đường kém và nguy cơ biến chứng liên quan đến bệnh đái tháo đường tăng lên. Do đó, việc sàng lọc thường quy về suy giảm nhận thức ở bệnh nhân cao tuổi mắc bệnh đái tháo đường týp 2 ngày càng được ủng hộ. Mục đích của nghiên cứu này là để tìm hiểu xem những người mắc bệnh đái tháo đường týp 2 và suy giảm nhận thức được phát hiện qua sàng lọc có sử dụng các dịch vụ chăm sóc sức khỏe cấp tính thường xuyên hơn những bệnh nhân không nghi ngờ bị suy giảm nhận thức hay không. Phương pháp Những người mắc bệnh đái tháo đường týp 2 ≥ 70 tuổi đã được sàng lọc về suy giảm nhận thức trong chăm sóc ban đầu. Chẩn đoán ở những người sàng lọc dương tính được xác định tại phòng khám trí nhớ. Thông tin về việc sử dụng dịch vụ chăm sóc sức khỏe cấp tính được thu thập trong 2 năm trước và 2 năm sau khi sàng lọc và so sánh với những người sàng lọc âm tính. Kết quả Có 154 người tham gia (38% là nữ, độ tuổi trung bình 76,7 ± 5,2 tuổi, thời gian mắc bệnh tiểu đường 8,7 ± 8,2 năm) được đưa vào nghiên cứu, 37 bệnh nhân bị suy giảm nhận thức, 117 bệnh nhân sàng lọc âm tính. Tỷ lệ những người tham gia bị suy giảm nhận thức sử dụng các dịch vụ chăm sóc sức khỏe cấp tính cao hơn so với những bệnh nhân sàng lọc âm tính; sự khác biệt này là có ý nghĩa đối với các dịch vụ ngoài giờ của bác sĩ đa khoa (56% so với 34% sử dụng dịch vụ này trong hơn 4 năm, p = 0,02). Số lần khám cấp cứu trung bình cũng cao hơn ở những người bị suy giảm nhận thức so với những người sàng lọc âm tính (2,2 ± 2,8 so với 1,4 ± 2,2 lần khám trong 4 năm, p < 0,05; 1,4 ± 2,2 so với 0,7 ± 1,5 lần khám trong 2 năm sau khi sàng lọc, p = 0,03). Các yếu tố có thể đóng vai trò trong nguy cơ sử dụng các dịch vụ chăm sóc sức khỏe cấp tính tăng này là trình độ học vấn thấp, có các triệu chứng trầm cảm (điểm CES-D ≥ 16), tự báo cáo các vấn đề trong việc tự chăm sóc và tự báo cáo các vấn đề trong các hoạt động thường ngày. Kết luận Những người mắc bệnh đái tháo đường týp 2 và suy giảm nhận thức được phát hiện qua sàng lọc sử dụng các dịch vụ chăm sóc sức khỏe cấp tính thường xuyên hơn."} +{"idx": 1910079, "en": "Achieving a pathologic complete response after neoadjuvant treatment is associated with improved prognosis in breast cancer. The CREATE-X trial demonstrated a significant survival improvement with capecitabine in patients with residual invasive disease after neoadjuvant chemotherapy, and the KATHERINE trial showed a significant benefit of trastuzumab-emtansine (TDM1) in human epidermal growth factor receptor 2 (HER2)-positive patients who did not achieve a pathologic complete response after neoadjuvant treatment, creating interesting alternatives of post-neoadjuvant treatments for high-risk patients. New agents are arising as therapeutic options for metastatic breast cancer such as the cyclin-dependent kinase inhibitors and the immune-checkpoint inhibitors, but none has been incorporated into the post-neoadjuvant setting so far. Evolving techniques such as next-generation sequencing and gene expression profiles have improved our knowledge regarding the biology of residual disease, and also on the mechanisms involved in treatment resistance. The present manuscript reviews the current available strategies, the ongoing trials, the potential biomarker-guided approaches and the perspectives for the post-neoadjuvant treatment and the management of residual disease after neoadjuvant treatment in breast cancer.", "gemini": "Việc đạt được đáp ứng hoàn toàn về mặt bệnh lý sau điều trị tân bổ trợ có liên quan đến tiên lượng cải thiện ở bệnh ung thư vú. Thử nghiệm CREATE-X đã chứng minh sự cải thiện đáng kể về khả năng sống sót với capecitabine ở những bệnh nhân bị bệnh xâm lấn còn sót lại sau hóa trị tân bổ trợ, và thử nghiệm KATHERINE cho thấy lợi ích đáng kể của trastuzumab-emtansine (TDM1) ở những bệnh nhân dương tính với thụ thể yếu tố tăng trưởng biểu bì ở người 2 (HER2) không đạt được đáp ứng hoàn toàn về mặt bệnh lý sau điều trị tân bổ trợ, tạo ra các lựa chọn thay thế thú vị về phương pháp điều trị sau tân bổ trợ cho những bệnh nhân có nguy cơ cao. Các tác nhân mới đang nổi lên như các lựa chọn điều trị cho bệnh ung thư vú di căn như các chất ức chế kinase phụ thuộc vào cyclin và các chất ức chế điểm kiểm soát miễn dịch, nhưng cho đến nay vẫn chưa có tác nhân nào được đưa vào phác đồ sau tân bổ trợ. Các kỹ thuật đang phát triển như giải trình tự thế hệ tiếp theo và hồ sơ biểu hiện gen đã cải thiện kiến thức của chúng ta về sinh học của bệnh còn sót lại, và cả về các cơ chế liên quan đến tình trạng kháng thuốc. Bản thảo hiện tại xem xét các chiến lược hiện có, các thử nghiệm đang diễn ra, các phương pháp tiếp cận có hướng dẫn sinh học tiềm năng và các viễn cảnh cho phương pháp điều trị sau tân bổ trợ và quản lý bệnh còn sót lại sau điều trị tân bổ trợ ở bệnh ung thư vú."} +{"idx": 1910080, "en": "BACKGROUND Acute renal failure (ARF) is a serious disease characterised by a rapid loss of renal functions due to nephrotoxic drug or ischemic insult. The clinical treatment approach such as dialysis techniques and continuous renal enhancement have grown rapidly during past decades. However, there is yet no significant effect in improving renal function. Hypoxia-preconditioned mesenchymal stem cells (HP-MSCs) have positive effects on the in vitro survival and stemness, in addition to angiogenic potential. AIM In this study, we aimed to analyse the effect of HP-MSCs administration in improving renal function, characterised by blood urea nitrogen (BUN) and creatinine level. METHODS A group of 15 male Wistar rats weighing 250 g to 300 g were used in this study (n = 5 for each group). Rats were randomly distributed into 3 groups: Vehicle control (Veh) as a control group, HP-MSCs and normoxia MSCs (N-MSCs) as the treatment group. Renal function was evaluated based on the BUN and creatinine levels using the colourimetric method on day 5 and 13. The histological analysis using HE staining was performed on day 13. RESULTS The result showed there is a significant decrease in BUN and creatinine level (p < 0.05). The histological analysis of renal tissue also showed a significant decrease between Veh and treatment group (p < 0.05). CONCLUSION Based on this study, we conclude that HP-MSCs have a superior beneficial effect than N-MSCs in improving renal function in an animal model of gentamicin-induced ARF.", "gemini": "BỐI CẢNH Suy thận cấp (ARF) là một căn bệnh nghiêm trọng đặc trưng bởi tình trạng mất chức năng thận nhanh chóng do thuốc độc thận hoặc thiếu máu cục bộ. Các phương pháp điều trị lâm sàng như kỹ thuật lọc máu và tăng cường chức năng thận liên tục đã phát triển nhanh chóng trong những thập kỷ qua. Tuy nhiên, vẫn chưa có tác dụng đáng kể nào trong việc cải thiện chức năng thận. Các tế bào gốc trung mô được tiền xử lý bằng thiếu oxy (HP-MSC) có tác dụng tích cực đối với khả năng sống sót và tính gốc in vitro, ngoài tiềm năng tạo mạch. MỤC ĐÍCH Trong nghiên cứu này, chúng tôi nhằm phân tích tác dụng của việc sử dụng HP-MSC trong việc cải thiện chức năng thận, đặc trưng bởi mức nitơ urê máu (BUN) và creatinin. PHƯƠNG PHÁP Một nhóm 15 con chuột Wistar đực nặng từ 250 g đến 300 g đã được sử dụng trong nghiên cứu này (n = 5 cho mỗi nhóm). Chuột được phân bố ngẫu nhiên vào 3 nhóm: Nhóm đối chứng dùng dung môi (Veh) làm nhóm đối chứng, nhóm HP-MSC và nhóm MSC thiếu oxy (N-MSC) làm nhóm điều trị. Chức năng thận được đánh giá dựa trên mức BUN và creatinin bằng phương pháp định lượng màu vào ngày thứ 5 và ngày thứ 13. Phân tích mô học bằng phương pháp nhuộm HE được thực hiện vào ngày thứ 13. KẾT QUẢ Kết quả cho thấy có sự giảm đáng kể về mức BUN và creatinin (p < 0,05). Phân tích mô học của mô thận cũng cho thấy sự giảm đáng kể giữa nhóm Veh và nhóm điều trị (p < 0,05). KẾT LUẬN Dựa trên nghiên cứu này, chúng tôi kết luận rằng HP-MSC có tác dụng có lợi vượt trội hơn N-MSC trong việc cải thiện chức năng thận ở mô hình động vật bị ARF do gentamicin gây ra."} +{"idx": 1910081, "en": "AIM The present study was undertaken to evaluate the possible protective role of thymoquinone on CCl4-induced hepatotoxicity. METHODS The activities of liver function enzymes and antioxidant enzymes were measured. Haematoxylin-Eosin staining was performed to analyze the live tissue alterations. Additionally, expression pattern of different proteins was evaluated through immunohistochemistry staining. RESULTS The antioxidants enzymes activities were decreased significantly in the CCl4 induced group whereas recovery/increase of antioxidant enzymes was observed when thymoquinone was given to the mice. Moreover, thymoquinone administration significantly decrease the serum levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), and serum aspartate aminotransferase (AST). Liver tissue alterations were noted in CCl4 treated group whereas treatment with thymoquinone significantly prevented the CCl4-induced histological alteration. The expression of PTEN protein was high in CCl4 plus thymoquinone treated group while the loss of PTEN protein expression was observed in CCl4 treated group. Moreover, high expression of P53 protein was noticed in CCl4 treated the group as compared to CCl4 plus thymoquinone group. Difference in expression pattern of PTEN and p53 protein in CCl4 group and thymoquinone plus CCl4 treated group was statically significant (p < 0.05). Besides, expression of VEGF was high in CCl4 treated group as well as thymoquinone plus CCl4 treated group and difference in expression pattern was statically insignificant (p > 0.05). CONCLUSION Our results suggest that thymoquinone can protect CCl4 induced liver damage and could be a preventive drug in the development of novel therapeutic agents for liver diseases.", "gemini": "MỤC TIÊU Nghiên cứu hiện tại được thực hiện để đánh giá vai trò bảo vệ có thể có của thymoquinone đối với độc tính gan do CCl4 gây ra. PHƯƠNG PHÁP Các hoạt động của enzym chức năng gan và enzym chống oxy hóa đã được đo. Nhuộm Hematoxylin-Eosin được thực hiện để phân tích các biến đổi mô sống. Ngoài ra, kiểu biểu hiện của các protein khác nhau đã được đánh giá thông qua nhuộm miễn dịch mô. KẾT QUẢ Các hoạt động của enzym chống oxy hóa giảm đáng kể trong nhóm gây ra bởi CCl4 trong khi phục hồi / tăng các enzym chống oxy hóa được quan sát thấy khi cho thymoquinone cho chuột. Hơn nữa, việc sử dụng thymoquinone làm giảm đáng kể nồng độ alanin aminotransferase (ALT), phosphatase kiềm (ALP) và aspartate aminotransferase (AST) trong huyết thanh. Các biến đổi mô gan được ghi nhận trong nhóm được điều trị bằng CCl4 trong khi điều trị bằng thymoquinone ngăn ngừa đáng kể sự thay đổi mô học do CCl4 gây ra. Sự biểu hiện của protein PTEN cao ở nhóm được điều trị bằng CCl4 cộng với thymoquinone trong khi sự mất biểu hiện protein PTEN được quan sát thấy ở nhóm được điều trị bằng CCl4. Hơn nữa, biểu hiện cao của protein P53 được ghi nhận ở nhóm được điều trị bằng CCl4 so với nhóm CCl4 cộng với thymoquinone. Sự khác biệt về kiểu biểu hiện của protein PTEN và p53 ở nhóm CCl4 và nhóm được điều trị bằng thymoquinone cộng với CCl4 là có ý nghĩa thống kê (p < 0,05). Bên cạnh đó, biểu hiện của VEGF cao ở nhóm được điều trị bằng CCl4 cũng như nhóm được điều trị bằng thymoquinone cộng với CCl4 và sự khác biệt về kiểu biểu hiện là không đáng kể về mặt thống kê (p> 0,05). KẾT LUẬN Kết quả của chúng tôi cho thấy thymoquinone có thể bảo vệ tổn thương gan do CCl4 gây ra và có thể là một loại thuốc phòng ngừa trong quá trình phát triển các tác nhân điều trị mới cho các bệnh về gan."} +{"idx": 1910082, "en": "BACKGROUND In healthy persons, iron acquisition, trafficking and storage are strictly regulated processes due to the lack of a physiological pathway for the excretion of excess iron from the body. The liver, the duodenum and the bone marrow are involved in the regulation of iron metabolism. MATERIAL Subject to the testing were 60 healthy volunteers who took part in clinical trials. METHODS Case histories, physical check-up and demographic data including people's heights and weights, laboratory studies and tests using medical equipment. RESULTS None of the healthy persons were reported to have shown any deviation from the reference values for the serum markers of iron metabolism tested with the exception of hepcidin. CONCLUSION In healthy persons, there was only a positive correlation between iron level and IBC, and feedback between hepcidin serum levels and transferrin saturation.", "gemini": "LÝ LỊCH Ở những người khỏe mạnh, quá trình thu nhận, vận chuyển và dự trữ sắt được điều chỉnh chặt chẽ do thiếu một con đường sinh lý để bài tiết sắt dư thừa ra khỏi cơ thể. Gan, tá tràng và tủy xương tham gia vào quá trình điều hòa chuyển hóa sắt. VẬT LIỆU Đối tượng thử nghiệm là 60 tình nguyện viên khỏe mạnh tham gia vào các thử nghiệm lâm sàng. PHƯƠNG PHÁP Tiền sử bệnh, kiểm tra sức khỏe và dữ liệu nhân khẩu học bao gồm chiều cao và cân nặng của mọi người, các xét nghiệm trong phòng thí nghiệm và các xét nghiệm sử dụng thiết bị y tế. KẾT QUẢ Không có người khỏe mạnh nào được báo cáo là có bất kỳ độ lệch nào so với các giá trị tham chiếu đối với các dấu ấn huyết thanh về chuyển hóa sắt được thử nghiệm ngoại trừ hepcidin. KẾT LUẬN Ở những người khỏe mạnh, chỉ có mối tương quan dương giữa mức sắt và IBC, và phản hồi giữa nồng độ hepcidin trong huyết thanh và độ bão hòa transferrin."} +{"idx": 1910083, "en": "BACKGROUND Repeated blood transfusions and hemolysis in β-Thalassemia major children lead to iron overload in various organs, including the brain which may cause neurodegeneration. AIM To evaluate intelligence quotient in children with β-thalassemia major and healthy counterparts and to assess risk factors that cause cognitive problems. SUBJECTS AND METHODS This case-control study was performed on 50 children aged 6-16 years old with β-thalassemia major as patients group and compared with 50 healthy children as a control group of matched age, sex, and social class. Cognitive functions were evaluated by using the Wechsler Intelligence Scale for Children. Serum ferritin and iron were measured by ELISA. RESULTS There were significantly lower mean performance and full-scale IQ scores of patients group in comparison with controls, whereas no significant differences between both groups as regards to a verbal IQ score. In thalassemic children, block design, comprehension and arithmetic were negatively correlated with age of disease onset, duration of illness and onset of chelation therapy. Serum iron and ferritin were negatively correlated with similarities and digit span. Serum iron levels were negatively correlated with performance IQ score. CONCLUSION Children with β-thalassemia major need to receive more academic attention and cognitive assessment to improve their IQ.", "gemini": "LÝ LỊCH Truyền máu nhiều lần và tan máu ở trẻ em mắc bệnh tan máu β-Thalassemia dẫn đến tình trạng thừa sắt ở nhiều cơ quan, bao gồm cả não, có thể gây thoái hóa thần kinh. MỤC TIÊU Đánh giá chỉ số thông minh ở trẻ em mắc bệnh tan máu β-Thalassemia và đối chứng khỏe mạnh và đánh giá các yếu tố nguy cơ gây ra các vấn đề về nhận thức. ĐỐI TƯỢNG VÀ PHƯƠNG PHÁP Nghiên cứu trường hợp chứng nhân này được thực hiện trên 50 trẻ em từ 6-16 tuổi mắc bệnh tan máu β-Thalassemia là nhóm bệnh nhân và so sánh với 50 trẻ em khỏe mạnh là nhóm đối chứng có độ tuổi, giới tính và giai cấp xã hội tương đồng. Chức năng nhận thức được đánh giá bằng Thang đo trí tuệ Wechsler dành cho trẻ em. Ferritin và sắt trong huyết thanh được đo bằng ELISA. KẾT QUẢ Có điểm IQ toàn diện và hiệu suất trung bình thấp hơn đáng kể ở nhóm bệnh nhân so với nhóm đối chứng, trong khi không có sự khác biệt đáng kể giữa hai nhóm về điểm IQ bằng lời nói. Ở trẻ em bị bệnh tan máu, thiết kế khối, hiểu biết và số học có mối tương quan âm với tuổi khởi phát bệnh, thời gian mắc bệnh và thời điểm bắt đầu điều trị thải sắt. Sắt và ferritin trong huyết thanh có mối tương quan âm với sự giống nhau và khoảng chữ số. Nồng độ sắt trong huyết thanh có mối tương quan âm với điểm IQ về hiệu suất. KẾT LUẬN Trẻ em mắc bệnh tan máu β-Thalassemia cần được quan tâm nhiều hơn về mặt học tập và đánh giá nhận thức để cải thiện chỉ số IQ của mình."} +{"idx": 1910084, "en": "BACKGROUND Cardiovascular modulation following meal consumption has been known. Little and conflicting data is present regarding electrocardiographic QT and QTc intervals after a meal, and status of ventricular repolarization following meal is not known comprehensively. AIM To inquire the electrocardiographic status of ventricular repolarisation thoroughly after lower and higher calorie meal consumption in a comparative manner. METHODS A group of 61 healthy individuals were studied before and after lunch. They were divided into two groups according to the calorie consumed (higher calorie and lower calorie; median 1580 and 900 kcals, respectively). Calorie consumed was estimated using dietary guidelines. Data was collected from 12-lead ECG both in a fasted state and 2nd postprandial hour for each participant. Parameters of ventricular repolarization, namely, JTp, Tp-e, QT, QTc intervals and their ratios, as well as RR intervals, were compared between fasted and postprandial states for every participant. RESULTS Tp-e and QTc intervals, and Tp-e/QTc ratio do not significantly change after both higher- and lower-calorie meals. JTp and QT intervals significantly shorten in both groups, regardless of the calorie consumed. While JTp shows a positive correlation with RR interval both before and after a meal in lower calorie intake group, no correlation was found with RR interval after a meal in higher calorie group. Logistic regression analysis revealed that higher calorie intake during a meal is a predictor for greater shortening in JTp and QT, compared to lower calorie meal. CONCLUSION Our study may guide future studies on ventricular repolarisation, particularly those conducted on various disease conditions or drug effect of cardiac electrophysiology.", "gemini": "BỐI CẢNH Điều chế tim mạch sau khi ăn đã được biết đến. Có ít dữ liệu và mâu thuẫn về khoảng QT và QTc trên điện tâm đồ sau bữa ăn và tình trạng tái phân cực thất sau bữa ăn không được biết đến một cách toàn diện. MỤC TIÊU Để tìm hiểu kỹ lưỡng tình trạng điện tâm đồ của sự tái phân cực thất sau khi tiêu thụ bữa ăn có lượng calo thấp hơn và cao hơn theo cách so sánh. PHƯƠNG PHÁP Một nhóm gồm 61 cá nhân khỏe mạnh đã được nghiên cứu trước và sau bữa trưa. Họ được chia thành hai nhóm theo lượng calo tiêu thụ (lượng calo cao hơn và lượng calo thấp hơn; trung bình lần lượt là 1580 và 900 kcal). Lượng calo tiêu thụ được ước tính bằng cách sử dụng các hướng dẫn về chế độ ăn uống. Dữ liệu được thu thập từ điện tâm đồ 12 chuyển đạo ở cả trạng thái nhịn ăn và giờ sau ăn thứ 2 đối với mỗi người tham gia. Các thông số tái phân cực thất, cụ thể là JTp, Tp-e, QT, khoảng QTc và tỷ lệ của chúng, cũng như khoảng RR, đã được so sánh giữa trạng thái nhịn ăn và sau ăn đối với mọi người tham gia. KẾT QUẢ Khoảng Tp-e và QTc và tỷ lệ Tp-e/QTc không thay đổi đáng kể sau cả bữa ăn có lượng calo cao hơn và thấp hơn. Khoảng JTp và QT ngắn lại đáng kể ở cả hai nhóm, bất kể lượng calo tiêu thụ. Trong khi JTp biểu hiện tương quan dương với khoảng RR trước và sau bữa ăn ở nhóm ăn ít calo, thì không tìm thấy tương quan với khoảng RR sau bữa ăn ở nhóm ăn nhiều calo. Phân tích hồi quy logistic cho thấy lượng calo nạp vào cao hơn trong bữa ăn là yếu tố dự báo sự rút ngắn lớn hơn ở JTp và QT, so với bữa ăn ít calo. KẾT LUẬN Nghiên cứu của chúng tôi có thể hướng dẫn các nghiên cứu trong tương lai về sự tái phân cực thất, đặc biệt là những nghiên cứu được tiến hành trên các tình trạng bệnh khác nhau hoặc tác dụng của thuốc đối với điện sinh lý tim."} +{"idx": 1910085, "en": "BACKGROUND Speech sound appears first in the child's speech development and is the primary means of expression. Articulation disorders can hinder the comprehensibility of children's speech. The speech, in turn, can limit the child's inclusion in the social and educational environment. AIM To establish frequency and distribution of lambdacism, rhotacism and sigmatism or their combination in preschool children and the frequency and distribution of these articulation disorders among boys and girls. MATERIAL AND METHODS A retrospective analysis of data from preventive examination for early diagnosis of impairments of hearing, speech and sounds in preschool children has been carried out. In the selected sample, only the data for children diagnosed with lambdacism, rhotacism, sigmatism or their combination are analysed. The data is statistically examined, represented in tables and figures and analysed descriptively. RESULTS In the analysed sample, the greater presence of lambdacism was observed before rhotacism and sigmatism. Most commonly, these three types of articulation disorder appear alone, as isolated cases, instead of a combination of two out of the three impairments. They are more common in boys than in girls. CONCLUSION Timely diagnosis and rehabilitation of lambdacism, rhotacism and sigmatism or their combination in preschool children will enable easier and faster integration of the children in the social and educational environment without leaving lasting consequences.", "gemini": "BỐI CẢNH Âm thanh lời nói xuất hiện đầu tiên trong quá trình phát triển lời nói của trẻ và là phương tiện biểu đạt chính. Rối loạn phát âm có thể cản trở khả năng hiểu được lời nói của trẻ. Đổi lại, lời nói có thể hạn chế sự hòa nhập của trẻ trong môi trường xã hội và giáo dục. MỤC TIÊU Để thiết lập tần suất và phân bố của chứng lambdacism, rhotacism và sigmatism hoặc sự kết hợp của chúng ở trẻ mẫu giáo và tần suất và phân bố của các rối loạn phát âm này giữa bé trai và bé gái. VẬT LIỆU VÀ PHƯƠNG PHÁP Một phân tích hồi cứu dữ liệu từ khám sàng lọc để chẩn đoán sớm các khiếm khuyết về thính giác, lời nói và âm thanh ở trẻ mẫu giáo đã được thực hiện. Trong mẫu được chọn, chỉ phân tích dữ liệu của những trẻ được chẩn đoán mắc chứng lambdacism, rhotacism, sigmatism hoặc sự kết hợp của chúng. Dữ liệu được kiểm tra thống kê, biểu diễn dưới dạng bảng và hình và được phân tích mô tả. KẾT QUẢ Trong mẫu được phân tích, sự hiện diện lớn hơn của chứng lambdacism đã được quan sát trước chứng rhotacism và sigmatism. Thông thường nhất, ba loại rối loạn phát âm này xuất hiện riêng lẻ, như những trường hợp riêng biệt, thay vì sự kết hợp của hai trong số ba khiếm khuyết. Chúng phổ biến hơn ở bé trai so với bé gái. KẾT LUẬN Chẩn đoán và phục hồi chức năng kịp thời đối với chứng lambdacism, rhotacism và sigmatism hoặc sự kết hợp của chúng ở trẻ mẫu giáo sẽ giúp trẻ hòa nhập dễ dàng và nhanh hơn vào môi trường xã hội và giáo dục mà không để lại hậu quả lâu dài."} +{"idx": 1910086, "en": "BACKGROUND Asthma is a chronic inflammatory disease of the airways that results from complex interactions between multiple environmental and genetic influences. In recent years, studies have observed an increase in caesarean section rates, and have suggested a strong association with the rapid increase in the incidence of childhood asthma that cannot be explained by genetic factors alone. In this case-control study, we investigate the association between the developments of childhood asthma with the mode of delivery. We also explored the relationship between mode of delivery and control of asthma. METHODS Two groups (509 pediatric patients in total) were assessed between January 1, 2017, and January 1, 2018. Part of these patients, 257 (50.4%) were asthmatic children visiting specialised clinics, and 252 (49.6%) controlled cases selected from a primary health care clinic from the same institution (control group). RESULTS The Chi-square test revealed a significant association between cesarean sections and bronchial asthma (OR, 1.483 [95% CI, 1.013-21.71]; P =0.042). However, the adjusted OR from our binary logistic regression model revealed this association to be insignificant (adjusted OR, 1.417 [95% CI, 0.885-2.269]; P =0.804). The value of the chi-square of the model shows that the overall model is statistically significant at 1%. The Nagelkerke R square indicates that 34.9% of the variation in having asthma is explained by the risk factors included in the model. CONCLUSION We do not believe that the rise in cesarean sections explains the increase in childhood bronchial asthma - at least not in our population. We also found no association between the mode of delivery and asthma control. We encourage further research into this topic, namely to recruit a larger number of patients, and to adjust for the significant risk factors found in our study.", "gemini": "BỐI CẢNH Hen suyễn là một bệnh viêm mãn tính của đường thở do sự tương tác phức tạp giữa nhiều yếu tố môi trường và di truyền. Trong những năm gần đây, các nghiên cứu đã quan sát thấy tỷ lệ mổ lấy thai tăng lên và cho thấy có mối liên quan chặt chẽ với sự gia tăng nhanh chóng về tỷ lệ mắc bệnh hen suyễn ở trẻ em mà không thể giải thích chỉ bằng các yếu tố di truyền. Trong nghiên cứu đối chứng này, chúng tôi tìm hiểu mối liên quan giữa sự phát triển của bệnh hen suyễn ở trẻ em với phương thức sinh. Chúng tôi cũng khám phá mối quan hệ giữa phương thức sinh và kiểm soát bệnh hen suyễn. PHƯƠNG PHÁP Hai nhóm (tổng cộng 509 bệnh nhi) đã được đánh giá trong khoảng thời gian từ ngày 1 tháng 1 năm 2017 đến ngày 1 tháng 1 năm 2018. Một phần trong số những bệnh nhân này, 257 (50,4%) là trẻ em bị hen suyễn đến khám tại các phòng khám chuyên khoa, và 252 (49,6%) là các trường hợp được kiểm soát được chọn từ một phòng khám chăm sóc sức khỏe ban đầu của cùng một cơ sở (nhóm đối chứng). KẾT QUẢ Thử nghiệm Chi-square cho thấy có mối liên quan đáng kể giữa mổ lấy thai và hen phế quản (OR, 1,483 [95% CI, 1,013-21,71]; P = 0,042). Tuy nhiên, OR đã điều chỉnh từ mô hình hồi quy logistic nhị phân của chúng tôi cho thấy mối liên quan này là không đáng kể (OR đã điều chỉnh, 1,417 [95% CI, 0,885-2,269]; P = 0,804). Giá trị chi bình phương của mô hình cho thấy mô hình tổng thể có ý nghĩa thống kê ở mức 1%. Bình phương R của Nagelkerke chỉ ra rằng 34,9% sự thay đổi trong việc mắc bệnh hen suyễn được giải thích bởi các yếu tố rủi ro có trong mô hình. KẾT LUẬN Chúng tôi không tin rằng sự gia tăng của các ca mổ lấy thai giải thích được sự gia tăng của bệnh hen phế quản ở trẻ em - ít nhất là không phải trong quần thể của chúng tôi. Chúng tôi cũng không tìm thấy mối liên quan nào giữa phương thức sinh và kiểm soát bệnh hen suyễn. Chúng tôi khuyến khích nghiên cứu sâu hơn về chủ đề này, cụ thể là tuyển dụng nhiều bệnh nhân hơn và điều chỉnh các yếu tố rủi ro đáng kể được tìm thấy trong nghiên cứu của chúng tôi."} +{"idx": 1910087, "en": "BACKGROUND Cleft palate, due to damage of the soft palate, leads to dysfunction, i.e., inappropriate closure of the velopharynx during speech production, thus resulting in velopharyngeal insufficiency which characterises with hypernasal speech and nasal air loss/emission during speech production. AIM To establish the relationship between the type of cleft according to the Veau classification and the degree of nasal air emission in the speech of patients with cleft using auditory-visual perceptual assessment procedures. MATERIAL AND METHODS A group of 40 patients with irregular speech aged 4 to 7, out of which 20 with cleft palate or cleft lip and palate, participated in the research. The Veau classification was used to classify the cleft severity, while an indirect instrumental examination was conducted with the See-Scape instrument to detect nasal air emission during the speech. RESULTS The respondents with cleft palate or cleft lip and palate of higher Veau class had a greater degree of nasal air emission during the speech. There is a positive, statistically significant correlation between the results obtained with the Veau classification of cleft lip and palate, and the degree of nasal air emission. The value of Spearman's coefficient of correlation is R = 0.46, and the calculated p-value is p = 0.04. CONCLUSION A more severe cleft type is associated with an increased degree of nasal air emission during the speech, and vice versa.", "gemini": "BỐI CẢNH Hở hàm ếch, do tổn thương vòm miệng mềm, dẫn đến rối loạn chức năng, tức là đóng không đúng chỗ của vòm họng hầu trong quá trình phát âm, do đó dẫn đến tình trạng hở vòm họng hầu đặc trưng bởi giọng nói quá mũi và mất/thoát khí mũi trong quá trình phát âm. MỤC ĐÍCH Thiết lập mối quan hệ giữa loại hở hàm ếch theo phân loại Veau và mức độ thoát khí mũi trong giọng nói của bệnh nhân hở hàm ếch bằng cách sử dụng các quy trình đánh giá nhận thức bằng thính giác-thị giác. VẬT LIỆU VÀ PHƯƠNG PHÁP Một nhóm 40 bệnh nhân nói không bình thường từ 4 đến 7 tuổi, trong đó có 20 bệnh nhân bị hở hàm ếch hoặc hở môi và hở hàm ếch, đã tham gia nghiên cứu. Phân loại Veau được sử dụng để phân loại mức độ nghiêm trọng của tình trạng hở hàm ếch, trong khi một cuộc kiểm tra dụng cụ gián tiếp được tiến hành bằng dụng cụ See-Scape để phát hiện tình trạng thoát khí mũi trong quá trình nói. KẾT QUẢ Những người trả lời bị hở hàm ếch hoặc hở môi và hở hàm ếch thuộc lớp Veau cao hơn có mức độ thoát khí mũi lớn hơn trong quá trình nói. Có mối tương quan tích cực, có ý nghĩa thống kê giữa kết quả thu được với phân loại Veau về hở môi và hở hàm ếch, và mức độ thoát khí mũi. Giá trị hệ số tương quan của Spearman là R = 0,46 và giá trị p được tính là p = 0,04. KẾT LUẬN Kiểu hở hàm ếch nghiêm trọng hơn có liên quan đến mức độ thoát khí mũi tăng lên trong quá trình nói, và ngược lại."} +{"idx": 1910088, "en": "BACKGROUND Stroke is a serious health condition and the leading cause of disability, including erectile dysfunction (ED). The mechanisms and factors that predict ED in stroke are not fully elucidated. Several studies have shown a relationship between the location of the lesion in stroke with the onset of ED. The left hemispheric lesion was believed to disrupt the parasympathetic nervous system which is responsible for the regulation of erection. Stroke attack in this region therefore can be the underlying reason for ED. However, there are still contradictory findings in this area, and very few studies attempted to look at this problem, particularly among Asian male. AIM We would like to evaluate the association between left hemisphere lesion after ischemic stroke and the subsequent risk of developing ED. METHODS The study was conducted in the neurology polyclinic of Sanglah and Wangaya Hospital from February to August 2013. Subjects are all post-stroke patients who visited the neurology polyclinic of Sanglah and Wangaya Hospital that met inclusion and exclusion criteria. RESULTS There is a significant positive correlation (p < 0.05) between left hemisphere lesions and ED in stroke patients with a correlation coefficient (r) = 0.361 which means weak correlation and p = 0.032. CONCLUSION Left hemisphere lesions positively correlated with ED in stroke patients. Further longitudinal research is needed to see whether left hemisphere lesion in post-stroke patients is a risk factor for ED.", "gemini": "BỐI CẢNH Đột quỵ là một tình trạng sức khỏe nghiêm trọng và là nguyên nhân hàng đầu gây ra tình trạng khuyết tật, bao gồm cả rối loạn cương dương (ED). Các cơ chế và yếu tố dự đoán ED trong đột quỵ vẫn chưa được làm sáng tỏ đầy đủ. Một số nghiên cứu đã chỉ ra mối quan hệ giữa vị trí tổn thương trong đột quỵ với tình trạng khởi phát ED. Tổn thương bán cầu trái được cho là làm gián đoạn hệ thần kinh phó giao cảm chịu trách nhiệm điều hòa sự cương cứng. Do đó, cơn đột quỵ tấn công ở vùng này có thể là nguyên nhân cơ bản gây ra ED. Tuy nhiên, vẫn còn những phát hiện trái ngược trong lĩnh vực này và rất ít nghiên cứu cố gắng xem xét vấn đề này, đặc biệt là ở nam giới châu Á. MỤC ĐÍCH Chúng tôi muốn đánh giá mối liên quan giữa tổn thương bán cầu trái sau đột quỵ thiếu máu cục bộ và nguy cơ phát triển ED sau đó. PHƯƠNG PHÁP Nghiên cứu được tiến hành tại phòng khám đa khoa thần kinh của Bệnh viện Sanglah và Wangaya từ tháng 2 đến tháng 8 năm 2013. Đối tượng nghiên cứu là tất cả bệnh nhân sau đột quỵ đã đến khám tại phòng khám đa khoa thần kinh của Bệnh viện Sanglah và Wangaya đáp ứng các tiêu chuẩn tuyển chọn và loại trừ. KẾT QUẢ Có mối tương quan dương có ý nghĩa (p < 0,05) giữa tổn thương bán cầu trái và ED ở bệnh nhân đột quỵ với hệ số tương quan (r) = 0,361 có nghĩa là tương quan yếu và p = 0,032. KẾT LUẬN Tổn thương bán cầu trái có tương quan dương với ED ở bệnh nhân đột quỵ. Cần nghiên cứu theo chiều dọc thêm để xem tổn thương bán cầu trái ở bệnh nhân sau đột quỵ có phải là yếu tố nguy cơ gây ED hay không."} +{"idx": 1910089, "en": "BACKGROUND Angioneurotic oedema (AE) is an unpredictable and dangerous disease directly threatening the patient's life due to a sudden onset of upper respiratory tract obstruction. The disease is associated with various causes and triggering factors, but little is known about the conditions that accompany AE. AIM The study aims to determine the age-specificities and the spectrum of concomitant diseases in patients with AE. MATERIAL AND METHODS The subjects of observation were 88 patients (53 women and 35 men) with angioneurotic oedema who underwent diagnostics and treatment in the Department of Occupational Diseases and Clinical Allergology of University hospital \"Saint George\"-Plovdiv. RESULTS The highest level of disease prevalence was found in the age group over 50 years, both in males (45.71%) and females (54.72%). We found that the most often concomitant diseases in our patients with AE are cardiovascular (33%). On second place are the patients with other accompanying conditions outside of the target groups (27.3%). Patients with AE and autoimmune thyroiditis were 14.8%, and those with AE and skeletal-muscle disorders-10.2%. Given the role of hereditary factors in this disease, the profession of the patients is considered insignificant. CONCLUSION Angioedema occurs in all age groups, but half of the cases are in people over 50 years of age. The most common concomitant diseases in angioedema are cardiovascular diseases.", "gemini": "BỐI CẢNH Phù mạch thần kinh (AE) là một căn bệnh nguy hiểm và khó lường đe dọa trực tiếp đến tính mạng của bệnh nhân do tình trạng tắc nghẽn đường hô hấp trên đột ngột. Bệnh liên quan đến nhiều nguyên nhân và yếu tố khởi phát khác nhau, nhưng người ta biết rất ít về các tình trạng đi kèm với AE. MỤC ĐÍCH Nghiên cứu nhằm xác định các đặc điểm về độ tuổi và phổ bệnh đi kèm ở những bệnh nhân mắc AE. VẬT LIỆU VÀ PHƯƠNG PHÁP Đối tượng quan sát là 88 bệnh nhân (53 nữ và 35 nam) bị phù mạch thần kinh đã được chẩn đoán và điều trị tại Khoa Bệnh nghề nghiệp và Dị ứng lâm sàng của Bệnh viện Đại học \"Saint George\"-Plovdiv. KẾT QUẢ Tỷ lệ mắc bệnh cao nhất được tìm thấy ở nhóm tuổi trên 50, ở cả nam giới (45,71%) và nữ giới (54,72%). Chúng tôi thấy rằng các bệnh đi kèm thường gặp nhất ở những bệnh nhân AE của chúng tôi là bệnh tim mạch (33%). Ở vị trí thứ hai là những bệnh nhân có các tình trạng đi kèm khác ngoài các nhóm mục tiêu (27,3%). Bệnh nhân AE và viêm tuyến giáp tự miễn là 14,8%, và những bệnh nhân AE và rối loạn cơ xương là 10,2%. Xét đến vai trò của các yếu tố di truyền trong căn bệnh này, nghề nghiệp của bệnh nhân được coi là không đáng kể. KẾT LUẬN Phù mạch xảy ra ở mọi nhóm tuổi, nhưng một nửa số trường hợp là ở những người trên 50 tuổi. Các bệnh đi kèm phổ biến nhất ở phù mạch là bệnh tim mạch."} +{"idx": 1910090, "en": "BACKGROUND Head and neck cancers include malignancies of the scalp and neck skin, nasal cavity, paranasal sinuses, oral cavity, salivary glands, pharynx and larynx. The term ABO secretor refers to people who secrete blood group antigens in their body fluids such as saliva, sweat, tears, semen, and serum. Non-secretors refer to those who do not secrete their blood group antigens in their body fluids. The lack of blood type antigens in body discharge increases the susceptibility to certain types of diseases and infection. AIM Our study aimed to investigate the relationship between the secretion of blood groups in the saliva of patients with head and neck cancers. MATERIAL AND METHODS This case-control study was performed on 110 people (57 patients with head and neck cancer who were referred to Imam Khomeini Hospital, Tehran and 53 cancer-free controls). Five ml of non-stimulated saliva were collected by the spitting method. By agglutination or lack of agglutination in the test tubes, we determined the patient's secretor or non-secretor condition. RESULTS In terms of secretor status, 52.7% of all samples were secretors. In the case group, 19 out of 57 cases (33.3%) were secretors, and 38 were non-secretors (66.7%). In the control group, 39 out of 53 cases (73.6%) were secretors, and 14 cases were non-secretors (26.4%). There was a significant difference in the percentage of non-secretors between the two groups (p = 0.00). CONCLUSION People with non-secretor status may be more prone to develop head and neck cancer. The presence of these antigens in saliva may have a protective effect.", "gemini": "BỐI CẢNH Ung thư đầu cổ bao gồm các khối u ác tính của da đầu và cổ, khoang mũi, xoang cạnh mũi, khoang miệng, tuyến nước bọt, hầu và thanh quản. Thuật ngữ tiết ABO dùng để chỉ những người tiết kháng nguyên nhóm máu trong dịch cơ thể của họ như nước bọt, mồ hôi, nước mắt, tinh dịch và huyết thanh. Người không tiết là những người không tiết kháng nguyên nhóm máu của họ trong dịch cơ thể. Việc thiếu kháng nguyên nhóm máu trong dịch tiết cơ thể làm tăng khả năng mắc một số loại bệnh và nhiễm trùng. MỤC ĐÍCH Nghiên cứu của chúng tôi nhằm điều tra mối quan hệ giữa việc tiết các nhóm máu trong nước bọt của bệnh nhân ung thư đầu cổ. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu trường hợp chứng chứng này được thực hiện trên 110 người (57 bệnh nhân ung thư đầu cổ được chuyển đến Bệnh viện Imam Khomeini, Tehran và 53 người đối chứng không bị ung thư). Thu 5 ml nước bọt không kích thích bằng phương pháp khạc nhổ. Bằng cách ngưng kết hoặc không ngưng kết trong các ống nghiệm, chúng tôi xác định tình trạng tiết hoặc không tiết của bệnh nhân. KẾT QUẢ Về tình trạng tiết, 52,7% trong tổng số các mẫu là người tiết. Trong nhóm trường hợp, 19 trong số 57 trường hợp (33,3%) là người tiết và 38 người không tiết (66,7%). Trong nhóm đối chứng, 39 trong số 53 trường hợp (73,6%) là người tiết và 14 trường hợp không tiết (26,4%). Có sự khác biệt đáng kể về tỷ lệ người không tiết giữa hai nhóm (p = 0,00). KẾT LUẬN Những người không tiết có thể dễ mắc ung thư đầu cổ hơn. Sự hiện diện của những kháng nguyên này trong nước bọt có thể có tác dụng bảo vệ."} +{"idx": 1910091, "en": "BACKGROUND Genetic variation in the genes that encode metformin transporters has been proven to cause pharmacokinetic variability and various glycemic response to metformin. Organic Cation Transporter (OCT) 1 protein encoded by the SLC22A1 gene is primarily responsible for the process of metformin influx to the hepatocytes as the target of antihyperglycemic action as well as metformin elimination through the renal. This study aimed to determine the allele frequency distribution of the SLC22A1 Met420del gene in OCT1 among the Javanese population, the largest ethnic group in Indonesia with T2DM. METHODS The research involved 100 adult patients from 9 healthcare facilities in Yogyakarta Province. The PCR-RFLP method was employed as a genotype analysis to detect polymorphism using 5'-AGGTTCACGGACTCTGTGCT-3' forward primer and 5'-AAGCTGGAGTGTGCGATCT-3' reverse primer. RESULTS No AA variant (wild type) type was found in the SLC22A1 Met420del gene, and only 4% of the subjects had Aa heterozygote type. The allele frequencies of A and a were 2.0% and 98.0% in all subjects, respectively. CONCLUSION The allele frequencies in the Javanese-Indonesian population were almost the same as those in the studies involving Japanese, Chinese-Han, and Asian-American populations. This study recommends further research on the correlation between the influence of methionine deletion at codon 420 on the variability of pharmacokinetic profiles and the glycemic response to metformin as well as the incidence of gastrointestinal intolerance due to metformin administration.", "gemini": "BỐI CẢNH Biến thể di truyền ở các gen mã hóa chất vận chuyển metformin đã được chứng minh là gây ra sự thay đổi dược động học và phản ứng đường huyết khác nhau đối với metformin. Protein vận chuyển cation hữu cơ (OCT) 1 do gen SLC22A1 mã hóa chủ yếu chịu trách nhiệm cho quá trình vận chuyển metformin vào tế bào gan như mục tiêu của tác dụng hạ đường huyết cũng như đào thải metformin qua thận. Nghiên cứu này nhằm xác định phân bố tần suất alen của gen SLC22A1 Met420del ở OCT1 trong quần thể người Java, nhóm dân tộc lớn nhất ở Indonesia mắc T2DM. PHƯƠNG PHÁP Nghiên cứu có sự tham gia của 100 bệnh nhân trưởng thành từ 9 cơ sở chăm sóc sức khỏe tại tỉnh Yogyakarta. Phương pháp PCR-RFLP được sử dụng như một phân tích kiểu gen để phát hiện đa hình bằng cách sử dụng đoạn mồi hướng dẫn 5'-AGGTTCACGGACTCTGTGCT-3' và đoạn mồi ngược 5'-AAGCTGGAGTGTGCGATCT-3'. KẾT QUẢ Không tìm thấy kiểu biến thể AA (kiểu hoang dã) nào trong gen SLC22A1 Met420del và chỉ có 4% số đối tượng có kiểu dị hợp tử Aa. Tần suất alen của A và a lần lượt là 2,0% và 98,0% ở tất cả các đối tượng. KẾT LUẬN Tần suất alen trong quần thể người Java-Indonesia gần giống với tần suất trong các nghiên cứu liên quan đến quần thể người Nhật, người Hoa-Hán và người Mỹ gốc Á. Nghiên cứu này khuyến nghị nên tiếp tục nghiên cứu về mối tương quan giữa ảnh hưởng của việc xóa methionine ở codon 420 đối với sự thay đổi của các đặc điểm dược động học và phản ứng đường huyết đối với metformin cũng như tỷ lệ không dung nạp đường tiêu hóa do dùng metformin."} +{"idx": 1910092, "en": "BACKGROUND Primary mediastinal seminomas most commonly occur in young men, and they are localised in the anterior mediastinum. CASE PRESENTATION The presented study is a case report of a 34-year-old man suffering from a mediastinal tumour in size of 19 cm, with pleural and pericardial effusion. The patient complains of cough, difficulty breathing, weight loss, and pronounced tiredness. CT of lungs and biopsy of the mediastinal change was performed. The histopathological analysis was in favour of a primary mediastinal seminoma. The patient initially had pronouncedly increased levels of LDH and β-hCG tumour marker. Pericardiocentesis was realised due to threatening tamponade of the heart, followed by 4 cycles of chemotherapy by BEP protocol. Following 2 cycles of chemotherapy, normalisation of LDH and β-HCG levels and significantly improved the clinical condition in the patient was found. Upon completion of 4 chemotherapy cycles by BEP protocol, the patient performed an FDG-PET scan with partial response and reduced dimension of a primary tumour in the mediastinum. Radiotherapy of residual tumour mass up to a total dose of 40Gy in 20 fractions was realised. Control FDG-PET scan had a finding of complete response to a tumour and absence of FDG uptake. The last follow-up examination was performed in October 2018, and the patient was disease-free for 54 months. CONCLUSION Multimodality treatment approach of chemotherapy followed by radiation consolidation ensured long-term survival in primary advanced mediastinal seminoma.", "gemini": "BỐI CẢNH U nguyên bào trung thất nguyên phát thường gặp nhất ở nam giới trẻ và chúng khu trú ở trung thất trước. TRÌNH BÀY BỆNH ÁN Nghiên cứu được trình bày là báo cáo trường hợp về một người đàn ông 34 tuổi mắc khối u trung thất có kích thước 19 cm, có tràn dịch màng phổi và màng tim. Bệnh nhân than phiền về ho, khó thở, sụt cân và mệt mỏi rõ rệt. Đã chụp CT phổi và sinh thiết tổn thương trung thất. Phân tích mô bệnh học ủng hộ u nguyên bào trung thất nguyên phát. Ban đầu, bệnh nhân có mức tăng rõ rệt các dấu ấn khối u LDH và β-hCG. Chọc màng tim được thực hiện do tình trạng ép tim đe dọa, sau đó là 4 chu kỳ hóa trị theo phác đồ BEP. Sau 2 chu kỳ hóa trị, thấy tình trạng bình thường hóa mức LDH và β-HCG và cải thiện đáng kể tình trạng lâm sàng ở bệnh nhân. Sau khi hoàn thành 4 chu kỳ hóa trị theo phác đồ BEP, bệnh nhân đã thực hiện chụp FDG-PET với đáp ứng một phần và giảm kích thước của khối u nguyên phát ở trung thất. Xạ trị khối u còn sót lại với tổng liều 40Gy trong 20 phân đoạn đã được thực hiện. Chụp FDG-PET kiểm tra có kết quả là đáp ứng hoàn toàn với khối u và không thấy hấp thụ FDG. Lần khám theo dõi cuối cùng được thực hiện vào tháng 10 năm 2018 và bệnh nhân không còn bệnh trong 54 tháng. KẾT LUẬN Phương pháp điều trị đa mô thức gồm hóa trị tiếp theo là củng cố bằng xạ trị đảm bảo sự sống còn lâu dài ở u nguyên bào trung thất tiến triển nguyên phát."} +{"idx": 1910093, "en": "BACKGROUND In 1882, the German pathologist Friedrich Daniel von Recklinghausen described a series of patients with a combination of cutaneous lesions and tumours of the peripheral and central nervous system. Succeeding this paper, all of the patients with similar symptoms were given the diagnosis \"von Recklinghausen disease\". In the 20th century, a distinction was made between Neurofibromatosis type 1 (NF1) and Neurofibromatosis type 2 (NF2) with the help of molecular testing. CASE REPORT We are presenting the results from multiple surgical esthetic and reconstructive surgical procedures performed on a female patient with severe congenital neurofibromatosis during 15 years (2000-2015). The external appearance of our patient was not reflected in the general public's beauty standards. Convinced that she was unusual and unaccepted by the society, she gathered all of the strength and became our patient at 15 years of age. CONCLUSION Transforming the patient's life in the next fifteen years improved her overall health and her life quality.", "gemini": "BỐI CẢNH Năm 1882, nhà bệnh học người Đức Friedrich Daniel von Recklinghausen đã mô tả một loạt các bệnh nhân có tổn thương da và khối u kết hợp ở hệ thần kinh ngoại biên và trung ương. Tiếp theo bài báo này, tất cả các bệnh nhân có triệu chứng tương tự đều được chẩn đoán là \"bệnh von Recklinghausen\". Vào thế kỷ 20, người ta đã phân biệt giữa Bệnh u sợi thần kinh loại 1 (NF1) và Bệnh u sợi thần kinh loại 2 (NF2) với sự trợ giúp của xét nghiệm phân tử. BÁO CÁO CA BỆNH Chúng tôi trình bày kết quả từ nhiều thủ thuật phẫu thuật thẩm mỹ và tái tạo được thực hiện trên một bệnh nhân nữ bị bệnh u sợi thần kinh bẩm sinh nghiêm trọng trong 15 năm (2000-2015). Vẻ ngoài của bệnh nhân của chúng tôi không phản ánh các tiêu chuẩn về vẻ đẹp của công chúng. Tin rằng mình khác thường và không được xã hội chấp nhận, cô đã tập hợp tất cả sức mạnh và trở thành bệnh nhân của chúng tôi ở tuổi 15. KẾT LUẬN Việc thay đổi cuộc sống của bệnh nhân trong mười lăm năm tiếp theo đã cải thiện sức khỏe tổng thể và chất lượng cuộc sống của cô ấy."} +{"idx": 1910094, "en": "BACKGROUND Number of reported cases of human dirofilariasis in the last couple of decades has been increasing. Dogs are the main reservoir hosts, while various mosquito species represent/serve as vectors. Human infections are caused by two different parasites: Dirofilaria immitis and Dirofilaria repens. Dirofilaria repens is currently considered to be one of the most rapidly-spreading human and animal parasite species in Europe. Clinical features are characterised by subcutaneous or ocular lesions which can persist for months without any symptoms. The disease is considered to be endemic in Mediterranean countries, central Asia, Israel, and Sri Lanka with increased incidence in Central and East Europe. Apart from the local population, tourists are also susceptible to this disease. Export of domestic animals can transfer parasites between different countries. This disease is rare in Croatia. CASE PRESENTATION We are presenting a case of a 58-year-old female patient with dirofilariasis of the genital region caused by Dirofilaria repens, confirmed with PCR and sequencing. CONCLUSION From the clinician point of view, subcutaneous dirofilariasis should be considered as a differential diagnosis in cases of long-lasting subcutaneous swellings.", "gemini": "BỐI CẢNH Số lượng các trường hợp nhiễm giun chỉ ở người được báo cáo trong vài thập kỷ qua đã tăng lên. Chó là vật chủ chứa mầm bệnh chính, trong khi các loài muỗi khác nhau đóng vai trò là vật trung gian truyền bệnh. Nhiễm trùng ở người là do hai loại ký sinh trùng khác nhau gây ra: Dirofilaria immitis và Dirofilaria repens. Dirofilaria repens hiện được coi là một trong những loài ký sinh trùng ở người và động vật lây lan nhanh nhất ở Châu Âu. Các đặc điểm lâm sàng được đặc trưng bởi các tổn thương dưới da hoặc ở mắt có thể kéo dài trong nhiều tháng mà không có bất kỳ triệu chứng nào. Bệnh được coi là đặc hữu ở các nước Địa Trung Hải, Trung Á, Israel và Sri Lanka với tỷ lệ mắc tăng ở Trung và Đông Âu. Ngoài người dân địa phương, du khách cũng dễ mắc bệnh này. Việc xuất khẩu động vật trong nước có thể truyền ký sinh trùng giữa các quốc gia khác nhau. Bệnh này rất hiếm gặp ở Croatia. TRÌNH BÀY CA BỆNH Chúng tôi trình bày một trường hợp bệnh nhân nữ 58 tuổi bị nhiễm giun chỉ ở vùng sinh dục do Dirofilaria repens gây ra, được xác nhận bằng PCR và giải trình tự. KẾT LUẬN Theo quan điểm của bác sĩ lâm sàng, nhiễm giun chỉ dưới da nên được coi là chẩn đoán phân biệt trong các trường hợp sưng dưới da kéo dài."} +{"idx": 1910095, "en": "BACKGROUND De novo pulmonary embolism (DNPE) is a term used when pulmonary embolism (PE) occur in the absence of deep vein thrombosis (DVT). Most DNPE cases occur in a patient who had a recent injury to the chest. CASE PRESENTATION However, here we report a case of DNPE with a slightly different presentation where there is no preceding trauma and has symptoms that mimic severe pneumonia. He presented with high fever, dyspnoea and pleuritic chest pain. Despite on 10 L of oxygen supplementation via high flow mask and already given bolus intravenous antibiotic, the patient still tachypnoeic and was persistently in type I respiratory failure. His chest X-ray showed consolidative changes. Upon further investigation revealed no evidence of DVT on Doppler ultrasound and normal D-dimer level. Due to the high index of suspicion by the attending physician, PE was suspected and later confirmed with computed tomography pulmonary angiography scan. He was successfully treated with anticoagulation therapy. The objective of this case report is to share the difficult experience of diagnosing PE when the presentation highly atypical and mimics severe pneumonia. CONCLUSION And with such a masquerading presentation, one can easily miss the diagnosis. To the best of our knowledge, there are very few similar cases reported.", "gemini": "BỐI CẢNH Thuyên tắc phổi mới (DNPE) là một thuật ngữ được sử dụng khi thuyên tắc phổi (PE) xảy ra khi không có huyết khối tĩnh mạch sâu (DVT). Hầu hết các trường hợp DNPE xảy ra ở bệnh nhân bị chấn thương ngực gần đây. TRÌNH BÀY CA BỆNH Tuy nhiên, ở đây chúng tôi báo cáo một trường hợp DNPE với biểu hiện hơi khác, không có chấn thương trước đó và có các triệu chứng giống như viêm phổi nặng. Ông đến khám với tình trạng sốt cao, khó thở và đau ngực kiểu màng phổi. Mặc dù đã bổ sung 10 lít oxy qua mặt nạ lưu lượng cao và đã tiêm kháng sinh tĩnh mạch, nhưng bệnh nhân vẫn thở nhanh và liên tục bị suy hô hấp loại I. Chụp X-quang ngực cho thấy có thay đổi dạng đông đặc. Khi điều tra thêm cho thấy không có bằng chứng về DVT trên siêu âm Doppler và mức D-dimer bình thường. Do bác sĩ điều trị có chỉ số nghi ngờ cao nên nghi ngờ có PE và sau đó được xác nhận bằng chụp cắt lớp vi tính phổi có tiêm thuốc cản quang. Ông đã được điều trị thành công bằng liệu pháp chống đông. Mục đích của báo cáo trường hợp này là chia sẻ kinh nghiệm khó khăn trong việc chẩn đoán PE khi biểu hiện rất không điển hình và giống như viêm phổi nặng. KẾT LUẬN Và với cách trình bày như vậy, người ta có thể dễ dàng bỏ sót chẩn đoán. Theo hiểu biết của chúng tôi, có rất ít trường hợp tương tự được báo cáo."} +{"idx": 1910096, "en": "BACKGROUND Acne inversa as a chronic inflammatory disorder can be divided into three stages according to Hurley's classification. It affects the axillary and anogenital region predominantly, and its chronic course of development is associated with a major negative impact on quality of life, especially in young patients. We discuss the different types of treatment in patients with acne inversa and the benefits of two-stage surgical treatment by serial excisions with primary wound closure under local anaesthesia. CASE REPORT We present a 28-year-old man with hidradenitis suppurativa stage I in the right axillary region and also in the pubic area. The patient is an active smoker. The patient was treated with Rifampicin 2x 300mg / day without any particular effect and preoperatively, systemic therapy with Clindamycin 4x 600mg / day was performed, combined with daily dressings with jodasept ointment for 7 days. The patient was treated through two surgical sessions under local anaesthesia with elliptical excision of the lesions located in the right axillary and the pubic area. Both of the two surgical defects were initially closed with single interrupted sutures. Histological examination of both lesions revealed the presence of suppurative folliculitis. CONCLUSION The literature describes various methods for treating acne inversa which include both systemic and local approaches. However, it is considered that drug therapy achieves only a temporary improvement in patients with hidradenitis suppurativa. For this reason, the surgical treatment of acne inversa is indicated as the only curative treatment, especially for recurrent lesions and serial excisions under local anaesthesia, followed by primary wound closure is a valuable treatment for patients with mild to moderate HS (Hurly stage I & II).", "gemini": "BỐI CẢNH Bệnh mụn mủ ngược là một rối loạn viêm mạn tính có thể được chia thành ba giai đoạn theo phân loại của Hurley. Bệnh ảnh hưởng chủ yếu đến vùng nách và hậu môn sinh dục, và quá trình phát triển mạn tính của bệnh có liên quan đến tác động tiêu cực lớn đến chất lượng cuộc sống, đặc biệt là ở những bệnh nhân trẻ tuổi. Chúng tôi thảo luận về các loại điều trị khác nhau ở những bệnh nhân bị mụn mủ ngược và những lợi ích của phương pháp điều trị phẫu thuật hai giai đoạn bằng cách cắt bỏ nhiều lần với khâu đóng vết thương ban đầu dưới gây tê tại chỗ. BÁO CÁO CA BỆNH Chúng tôi trình bày một người đàn ông 28 tuổi bị viêm tuyến mồ hôi mủ giai đoạn I ở vùng nách phải và ở vùng mu. Bệnh nhân là người hút thuốc chủ động. Bệnh nhân được điều trị bằng Rifampicin 2x 300mg / ngày mà không có bất kỳ tác dụng cụ thể nào và trước khi phẫu thuật, bệnh nhân đã được điều trị toàn thân bằng Clindamycin 4x 600mg / ngày, kết hợp với băng bó hàng ngày bằng thuốc mỡ jodasept trong 7 ngày. Bệnh nhân được điều trị qua hai lần phẫu thuật dưới gây tê tại chỗ với cắt bỏ hình elip các tổn thương nằm ở vùng nách phải và vùng mu. Cả hai khiếm khuyết phẫu thuật đều được khâu đóng ban đầu bằng chỉ khâu rời đơn. Kiểm tra mô học của cả hai tổn thương cho thấy có viêm nang lông mủ. KẾT LUẬN Tài liệu mô tả các phương pháp khác nhau để điều trị mụn mủ ngược bao gồm cả phương pháp toàn thân và tại chỗ. Tuy nhiên, người ta cho rằng liệu pháp dùng thuốc chỉ đạt được sự cải thiện tạm thời ở những bệnh nhân bị viêm tuyến mồ hôi mủ. Vì lý do này, điều trị phẫu thuật mụn mủ ngược được chỉ định là phương pháp điều trị duy nhất có khả năng chữa khỏi, đặc biệt là đối với các tổn thương tái phát và cắt bỏ nhiều lần dưới gây tê tại chỗ, sau đó khâu đóng vết thương ban đầu là phương pháp điều trị có giá trị cho những bệnh nhân bị HS nhẹ đến trung bình (Giai đoạn I & II của Hurly)."} +{"idx": 1910097, "en": "BACKGROUND Visceral artery aneurysms are rare conditions. The incidence of hepatic artery aneurysm (HAA) accounts for approximately 20% of all splanchnic aneurysms. HAA can become a life-threatening situation because there is a great risk of rupture when it grows more than 2 cm in diameter. CASE PRESENTATION In this article, we describe a case of a 54-year-old female patient with large hepatic artery aneurysm which was incidentally founded. From symptoms, she had abdominal discomfort slightly worsening after meals, frequent pain in mesogastrium and reported some unexplained weight loss in the last few months. The lesion was detected by CT examination of the abdomen and pelvis. CONCLUSION The aneurysm was successfully treated at our department with the endovascular approach, by covering the aneurysmal sac with two stents, one closed cell and one double-layer micromesh new generation stent as a flow diverting option..", "gemini": "BỐI CẢNH Phình động mạch tạng là tình trạng hiếm gặp. Tỷ lệ phình động mạch gan (HAA) chiếm khoảng 20% tổng số phình động mạch tạng. HAA có thể trở thành tình trạng đe dọa tính mạng vì có nguy cơ vỡ lớn khi đường kính của nó tăng hơn 2 cm. TRÌNH BÀY CA BỆNH Trong bài viết này, chúng tôi mô tả một trường hợp bệnh nhân nữ 54 tuổi bị phình động mạch gan lớn được phát hiện tình cờ. Về các triệu chứng, bệnh nhân bị khó chịu ở bụng, tình trạng này hơi nặng hơn sau bữa ăn, thường xuyên đau ở vùng thượng vị và báo cáo rằng bị sụt cân không rõ nguyên nhân trong vài tháng qua. Tổn thương được phát hiện bằng chụp CT ổ bụng và khung chậu. KẾT LUẬN Phình động mạch đã được điều trị thành công tại khoa của chúng tôi bằng phương pháp tiếp cận nội mạch, bằng cách phủ lên túi phình hai stent, một stent ô kín và một stent thế hệ mới bằng lưới siêu nhỏ hai lớp như một lựa chọn chuyển hướng dòng chảy.."} +{"idx": 1910098, "en": "AIM The present study aimed to investigate the inhibitory effect of Lactobacillus rhamnosus (B-445) as a probiotics irrigant on the growth of Enterococcus faecalis. METHODS Forty-two extracted single human canal anterior teeth were prepared with rotary instrumentation and sterilised. Teeth were divided into 3 groups according to the type of irrigant, N = 14. Three experimental groups were inoculated with E. faecalis and cultured for 21 days before use; Group 1 was 2.5% NaOCl (positive control), Group 2 was saline (negative control), Group 3 was the experimental probiotic irrigant. Paper point sampling of the canals of each group was obtained before irrigation (S1), immediately after irrigation (S2) and after 24 hours (post irrigation samples) (S3) to determine remaining colony forming units for E. faecalis. Also, Colony counts for L. rhamnosus in Group 3 after immediate irrigation, as well as 24 hours post irrigation, was performed to determine the survival profile of these bacteria in infected root canal with E. faecalis. RESULTS The NaOCl irrigant group had the lowest mean value of (log 10 CFU/mL) of E. faecalis after immediate irrigation and after 24 hrs post irrigation followed by the probiotic group, while the highest mean value was the saline group (P ≤ 0.001). The survival profile for L. rhamnosus in Group 3 after immediate irrigation and post-irrigation were slightly higher than for E. faecalis (P ≤ 0.001). CONCLUSION Lactobacillus rhamnosus which revealed a potential inhibitory effect on the growth of Enterococcus faecalis, could be used as a new natural, safe probiotic irrigant agent.", "gemini": "MỤC ĐÍCH Nghiên cứu hiện tại nhằm mục đích điều tra tác dụng ức chế của Lactobacillus rhamnosus (B-445) như một chất tưới rửa lợi khuẩn đối với sự phát triển của Enterococcus faecalis. PHƯƠNG PHÁP Bốn mươi hai răng cửa trước của người đã nhổ được chuẩn bị bằng dụng cụ quay và khử trùng. Răng được chia thành 3 nhóm theo loại chất tưới rửa, N = 14. Ba nhóm thực nghiệm được tiêm chủng E. faecalis và nuôi cấy trong 21 ngày trước khi sử dụng; Nhóm 1 là NaOCl 2,5% (kiểm soát dương tính), Nhóm 2 là nước muối (kiểm soát âm tính), Nhóm 3 là chất tưới rửa lợi khuẩn thực nghiệm. Lấy mẫu giấy điểm của ống tủy của mỗi nhóm trước khi tưới rửa (S1), ngay sau khi tưới rửa (S2) và sau 24 giờ (mẫu sau khi tưới rửa) (S3) để xác định số đơn vị hình thành khuẩn lạc còn lại của E. faecalis. Ngoài ra, cũng tiến hành đếm khuẩn lạc của L. rhamnosus trong Nhóm 3 sau khi tưới rửa ngay lập tức, cũng như 24 giờ sau khi tưới rửa, để xác định hồ sơ sống sót của những vi khuẩn này trong ống tủy bị nhiễm trùng bởi E. faecalis. KẾT QUẢ Nhóm tưới rửa NaOCl có giá trị trung bình thấp nhất của (log 10 CFU/mL) của E. faecalis sau khi tưới rửa ngay lập tức và sau 24 giờ sau khi tưới rửa, tiếp theo là nhóm lợi khuẩn, trong khi nhóm nước muối có giá trị trung bình cao nhất (P ≤ 0,001). Hồ sơ sống sót của L. rhamnosus trong Nhóm 3 sau khi tưới rửa ngay lập tức và sau khi tưới rửa cao hơn một chút so với E. faecalis (P ≤ 0,001). KẾT LUẬN Lactobacillus rhamnosus cho thấy tác dụng ức chế tiềm tàng đối với sự phát triển của Enterococcus faecalis, có thể được sử dụng như một tác nhân tưới rửa lợi khuẩn tự nhiên, an toàn mới."} +{"idx": 1910099, "en": "AIM Recent studies have demonstrated that low-level laser therapy (LLLT) can accelerate orthodontic tooth movement. However, there is still controversy about the optimum parameters that can cause acceleration. The present study was designed to examine two different doses of LLLT in the acceleration of orthodontic tooth movement and their effect on relapse. METHODS An orthodontic appliance was designed to induce tooth movement on lower incisors of rabbits. The in-al-as diode laser was used to radiate different groups of rabbits according to a specific protocol. The amount of tooth movement was measured for 21 days, and then the orthodontic appliance was removed, and the relapse was measured till day 28. RESULTS The amount of tooth movement was significantly greater in the group with irradiation of low dose in comparison with the high dose group and the control group. The relapse was greatest in the group of low dose irradiation and least in the control group. CONCLUSION The findings suggest that LLLT with a low dose accelerate the orthodontic tooth movement while LLLT with a high dose was not able to have the same effect. LLLT had a reverse effect on relapse tendency; an increase in the relapse tendency was seen with low dose irradiation.", "gemini": "MỤC TIÊU Các nghiên cứu gần đây đã chứng minh rằng liệu pháp laser mức độ thấp (LLLT) có thể đẩy nhanh quá trình di chuyển răng chỉnh nha. Tuy nhiên, vẫn còn tranh cãi về các thông số tối ưu có thể gây ra sự tăng tốc. Nghiên cứu hiện tại được thiết kế để kiểm tra hai liều LLLT khác nhau trong việc tăng tốc độ di chuyển răng chỉnh nha và tác dụng của chúng đối với tình trạng tái phát. PHƯƠNG PHÁP Một thiết bị chỉnh nha được thiết kế để tạo ra chuyển động răng trên răng cửa dưới của thỏ. Điốt laser in-al-as được sử dụng để chiếu xạ các nhóm thỏ khác nhau theo một giao thức cụ thể. Lượng di chuyển răng được đo trong 21 ngày, sau đó tháo bỏ dụng cụ chỉnh nha và đo độ tái phát cho đến ngày 28. KẾT QUẢ Lượng di chuyển răng lớn hơn đáng kể ở nhóm chiếu xạ liều thấp so với nhóm liều cao và nhóm đối chứng. Tình trạng tái phát lớn nhất ở nhóm chiếu xạ liều thấp và ít nhất ở nhóm đối chứng. KẾT LUẬN Các phát hiện cho thấy LLLT với liều thấp làm tăng tốc độ di chuyển răng chỉnh nha trong khi LLLT với liều cao không có tác dụng tương tự. LLLT có tác dụng ngược lại đối với xu hướng tái phát; sự gia tăng xu hướng tái phát được thấy ở nhóm chiếu xạ liều thấp."} +{"idx": 1910100, "en": "BACKGROUND Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders, worldwide. Psychological disorders are common among patients with IBS. AIM This study aims to investigate the efficacy of acceptance and commitment therapy (ACT) matrix on depression and psychological capital of patients with IBS. MATERIAL AND METHODS In a quasi-experimental study, a total number of 30 patients with IBS were selected using convenience sampling. Those patients who meet the inclusion criteria were randomly assigned to control and experimental groups (15 patients in each group). Data were collected using the Beck Depression Inventory (BDI) and the Psychological Capital Questionnaire (PCQ). The experimental group was subjected to the acceptance and commitment therapy (ACT) amtrix, but the control group do not receive this treatment. Questionnaires were completed before (pre-test) and after (post-test) the intervention by patients in two groups. All patients in two groups responded to the questionnaires and returned them to the researcher. Data were analyzed using chi-square test, independent t-test, analysis of covariance (ANCOVA) and multivariate analysis of covariance (MANCOVA). RESULTS Analyzing the data showed that there were significant differences regarding depression and psychological capital between experimental and control group, before and after the study (p < 0.05). CONCLUSION Using ACT matrix is a useful modality to improve the depression and psychological capital among patients with IBS.", "gemini": "BỐI CẢNH Hội chứng ruột kích thích (IBS) là một trong những rối loạn chức năng tiêu hóa phổ biến nhất trên toàn thế giới. Rối loạn tâm lý thường gặp ở những bệnh nhân mắc IBS. MỤC TIÊU Nghiên cứu này nhằm mục đích điều tra hiệu quả của ma trận liệu pháp chấp nhận và cam kết (ACT) đối với chứng trầm cảm và vốn tâm lý của bệnh nhân IBS. VẬT LIỆU VÀ PHƯƠNG PHÁP Trong một nghiên cứu bán thực nghiệm, tổng số 30 bệnh nhân mắc IBS đã được chọn bằng phương pháp lấy mẫu thuận tiện. Những bệnh nhân đáp ứng các tiêu chuẩn tuyển chọn được phân ngẫu nhiên vào nhóm đối chứng và nhóm thực nghiệm (15 bệnh nhân ở mỗi nhóm). Dữ liệu được thu thập bằng cách sử dụng Bản kiểm kê trầm cảm Beck (BDI) và Bản câu hỏi về vốn tâm lý (PCQ). Nhóm thực nghiệm được áp dụng ma trận liệu pháp chấp nhận và cam kết (ACT), nhưng nhóm đối chứng không nhận được phương pháp điều trị này. Bệnh nhân ở hai nhóm đã hoàn thành bảng câu hỏi trước (tiền kiểm tra) và sau (hậu kiểm tra) khi can thiệp. Tất cả bệnh nhân ở hai nhóm đã trả lời bảng câu hỏi và trả lại cho nhà nghiên cứu. Dữ liệu được phân tích bằng kiểm định chi bình phương, kiểm định t độc lập, phân tích hiệp phương sai (ANCOVA) và phân tích hiệp phương sai đa biến (MANCOVA). KẾT QUẢ Phân tích dữ liệu cho thấy có sự khác biệt đáng kể về chứng trầm cảm và vốn tâm lý giữa nhóm thực nghiệm và nhóm đối chứng, trước và sau khi nghiên cứu (p < 0,05). KẾT LUẬN Sử dụng ma trận ACT là phương thức hữu ích để cải thiện chứng trầm cảm và vốn tâm lý ở những bệnh nhân mắc IBS."} +{"idx": 1910101, "en": "INTRODUCTION The urban air pollution will deteriorate globally, by 2050 outdoor particulate air pollution and ground-level ozone is projected to become the top cause of environmentally related deaths worldwide. AIM To assess the burden of diseases due to particulate air pollution and health benefits form the pollution reduction policies. METHODS Environmental burden of disease methodology has been applied. Environmental data for population exposure, total years of life lost from all causes, and relevant concentration-response functions have been used in estimation. RESULTS The estimated disease burden from all causes (excl. external) is 30,256 YLL (19,436-40,625 95% CI) in Skopje Region, and 10,343 YLL (6,224-14,785 95% CI) in Tetovo. In terms of cardiopulmonary mortality, the estimated disease burden is 9,282/100,000 in Tetovo, in the Skopje Region 3,784/100,000 respectively. Annually in Tetovo 1,645 years of healthy life are lost, while in the Skopje Region 3,936 due to lung cancer premature mortality. The estimated health gain is significant, for the three selected health outcomes if EU limit values are reached, 41-42% of the estimated burden in Skopje Region will be eliminated, and 74-77% in Tetovo. CONCLUSION the estimated impact of particulate air pollution on mortality is significant and not negligible. The same applies to the health and well-being of the population if the EU or WHO limit levels are reached.", "gemini": "GIỚI THIỆU Ô nhiễm không khí đô thị sẽ xấu đi trên toàn cầu, đến năm 2050, ô nhiễm không khí dạng hạt ngoài trời và ôzôn ở tầng mặt đất được dự đoán sẽ trở thành nguyên nhân hàng đầu gây tử vong liên quan đến môi trường trên toàn thế giới. MỤC TIÊU Đánh giá gánh nặng bệnh tật do ô nhiễm không khí dạng hạt và lợi ích sức khỏe từ các chính sách giảm ô nhiễm. PHƯƠNG PHÁP Đã áp dụng phương pháp luận gánh nặng bệnh tật do môi trường. Dữ liệu về môi trường về mức độ phơi nhiễm của dân số, tổng số năm mất đi của tất cả các nguyên nhân và các hàm đáp ứng nồng độ có liên quan đã được sử dụng để ước tính. KẾT QUẢ Gánh nặng bệnh tật ước tính từ tất cả các nguyên nhân (không bao gồm nguyên nhân bên ngoài) là 30.256 YLL (19.436-40.625 95% CI) ở Vùng Skopje và 10.343 YLL (6.224-14.785 95% CI) ở Tetovo. Về tỷ lệ tử vong do tim phổi, gánh nặng bệnh tật ước tính là 9.282/100.000 ở Tetovo, tương ứng là 3.784/100.000 ở Vùng Skopje. Hàng năm ở Tetovo mất 1.645 năm sống khỏe mạnh, trong khi ở Vùng Skopje mất 3.936 năm do tử vong sớm vì ung thư phổi. Lợi ích sức khỏe ước tính là rất đáng kể, đối với ba kết cục sức khỏe được chọn nếu đạt được các giá trị giới hạn của EU, 41-42% gánh nặng ước tính ở Vùng Skopje sẽ được loại bỏ và 74-77% ở Tetovo. KẾT LUẬN tác động ước tính của ô nhiễm không khí dạng hạt đối với tỷ lệ tử vong là đáng kể và không thể bỏ qua. Điều tương tự cũng áp dụng cho sức khỏe và hạnh phúc của người dân nếu đạt được các mức giới hạn của EU hoặc WHO."} +{"idx": 1910102, "en": "BACKGROUND Medical students are exposed to occupational health hazards in hospitals during their studies and lack sufficient education about infection control measures. Injury to medical students is a substantial problem and students have an increased risk of hepatitis B virus (HBV). To understand how medical students think about infection control, it is important to identify the strengths and weaknesses of their education. AIM To assess current knowledge, attitudes, and practice of medical students regarding HBV infection and control measures at Qassim University, Saudi Arabia. MATERIAL AND METHODS A cross-sectional study was conducted at a medical college. Participants completed a 39-item self-administered questionnaire assessing demographics, knowledge, attitudes, and practice. Item response frequencies were calculated. Responses were recorded into yes (strongly agree and agree) and no (neutral, disagree, and strongly disagree) answers. Correct responses were totalled and categorised as good or poor performance. A scale cut-off of less than 75% correct responses was considered poor, and 75% or more correct responses was considered good. Odds ratios and 95% confidence intervals were calculated, and the chi-square test was used for analysis. RESULTS A total of 21%, 41%, and 8% of students expressed good knowledge, attitudes, and practice, respectively. There was no statistically significant difference between males and females on knowledge (p = 0.089), attitudes (p = 0.829), and practice (p = 0.248). There was a statistically significant difference between academic years on knowledge (p = 0.0001), attitudes (p = 0.0001), and practice (p = 0.0001). CONCLUSION Most medical students have poor knowledge, attitudes, and practice regarding the risk of HBV infection. It is recommended that a policy is implemented for training on infection prevention for all medical students before they start clinical practice. Prevention programs about HBV infection should be instituted, and existing programs must be strengthened.", "gemini": "BỐI CẢNH Sinh viên y khoa tiếp xúc với các mối nguy hại sức khỏe nghề nghiệp trong bệnh viện trong quá trình học tập của họ và thiếu kiến thức đầy đủ về các biện pháp kiểm soát nhiễm trùng. Chấn thương ở sinh viên y khoa là một vấn đề đáng kể và sinh viên có nguy cơ mắc vi-rút viêm gan B (HBV) cao hơn. Để hiểu cách sinh viên y khoa nghĩ về kiểm soát nhiễm trùng, điều quan trọng là phải xác định được điểm mạnh và điểm yếu của nền giáo dục của họ. MỤC ĐÍCH Đánh giá kiến thức, thái độ và thực hành hiện tại của sinh viên y khoa liên quan đến nhiễm trùng HBV và các biện pháp kiểm soát tại Đại học Qassim, Ả Rập Saudi. VẬT LIỆU VÀ PHƯƠNG PHÁP Một nghiên cứu cắt ngang đã được tiến hành tại một trường cao đẳng y khoa. Những người tham gia đã hoàn thành một bảng câu hỏi tự quản gồm 39 câu để đánh giá thông tin nhân khẩu học, kiến thức, thái độ và thực hành. Tần suất trả lời mục đã được tính toán. Các phản hồi được ghi vào câu trả lời có (đồng ý mạnh mẽ và đồng ý) và không (trung lập, không đồng ý và không đồng ý mạnh mẽ). Các phản hồi chính xác đã được tổng hợp và phân loại là hiệu suất tốt hoặc kém. Điểm cắt thang điểm dưới 75% câu trả lời đúng được coi là kém và 75% trở lên câu trả lời đúng được coi là tốt. Tỷ lệ cược và khoảng tin cậy 95% đã được tính toán và sử dụng kiểm định chi bình phương để phân tích. KẾT QUẢ Tổng cộng 21%, 41% và 8% sinh viên thể hiện kiến thức, thái độ và thực hành tốt. Không có sự khác biệt có ý nghĩa thống kê giữa nam và nữ về kiến thức (p = 0,089), thái độ (p = 0,829) và thực hành (p = 0,248). Có sự khác biệt có ý nghĩa thống kê giữa các năm học về kiến thức (p = 0,0001), thái độ (p = 0,0001) và thực hành (p = 0,0001). KẾT LUẬN Hầu hết sinh viên y khoa có kiến thức, thái độ và thực hành kém về nguy cơ nhiễm HBV. Khuyến cáo nên thực hiện chính sách đào tạo về phòng chống nhiễm trùng cho tất cả sinh viên y khoa trước khi bắt đầu thực hành lâm sàng. Các chương trình phòng ngừa về nhiễm HBV nên được lập ra và các chương trình hiện có phải được tăng cường."} +{"idx": 1910103, "en": "BACKGROUND Choice theory, as a psychopathological model, postulated five basics, genetically and universally, needs. Accordingly, the rate of everybody happiness and quality of life is depended on the number of his needs fulfilling. Although some scales have been constructed to assess basic needs, they have proposed unity of needs for all human. AIM The present study was designed to construct a new scale, considering individualisation needs for each person; quality of life scale based on choice theory (QOLSCT). METHODS Using cluster sampling, six hundred (49% female and 51% male) postgraduate students were selected. One hundred fifty participants also filled SF-36 and GHQ, and 80 participants refilled QOLSCT with four weeks' interval again. RESULTS Cronbach's alpha, split-half and test re-test (one month) reliability scores were 0.78, 0.75, and 0.92 successively. The correlation between items and total scores range from 0.36 to 0.72, all with P values ≥ 0.0001. Confirmatory factor analysis showed satisfactory values of goodness-of-fit indices, RMSEA, CFI, NFI and GFI were, 0.05, 0.99, 0.99 and 0.99 respectively. Convergent and divergent validity also showed significant correlations. CONCLUSION The result of the present study showed that the new version of quality of life scale based on choice theory could be confirmed. The good level of reliability, fairly goodness of fit indexes, and very good convergent validity support this idea.", "gemini": "BỐI CẢNH Lý thuyết lựa chọn, như một mô hình bệnh lý tâm lý, đưa ra năm nhu cầu cơ bản, về mặt di truyền và phổ quát. Theo đó, tỷ lệ hạnh phúc và chất lượng cuộc sống của mỗi người phụ thuộc vào số lượng nhu cầu được đáp ứng. Mặc dù một số thang điểm đã được xây dựng để đánh giá các nhu cầu cơ bản, nhưng chúng đã đề xuất sự thống nhất về nhu cầu của tất cả mọi người. MỤC ĐÍCH Nghiên cứu hiện tại được thiết kế để xây dựng một thang điểm mới, xem xét nhu cầu cá nhân hóa cho từng người; thang điểm chất lượng cuộc sống dựa trên lý thuyết lựa chọn (QOLSCT). PHƯƠNG PHÁP Sử dụng phương pháp lấy mẫu theo cụm, sáu trăm (49% nữ và 51% nam) sinh viên sau đại học đã được lựa chọn. Một trăm năm mươi người tham gia cũng đã điền SF-36 và GHQ, và 80 người tham gia đã điền lại QOLSCT với khoảng thời gian bốn tuần một lần nữa. KẾT QUẢ Độ tin cậy của Cronbach's alpha, chia đôi và kiểm tra lại (một tháng) lần lượt là 0,78, 0,75 và 0,92. Hệ số tương quan giữa các mục và tổng điểm dao động từ 0,36 đến 0,72, tất cả đều có giá trị P ≥ 0,0001. Phân tích nhân tố xác nhận cho thấy các giá trị thỏa đáng của các chỉ số độ phù hợp, RMSEA, CFI, NFI và GFI lần lượt là 0,05, 0,99, 0,99 và 0,99. Độ tin hợp hội tụ và độ tin phân kỳ cũng cho thấy các mối tương quan có ý nghĩa. KẾT LUẬN Kết quả của nghiên cứu hiện tại cho thấy phiên bản mới của thang điểm chất lượng cuộc sống dựa trên lý thuyết lựa chọn có thể được xác nhận. Mức độ tin cậy tốt, chỉ số độ phù hợp khá tốt và độ tin hợp hội tụ rất tốt ủng hộ ý tưởng này."} +{"idx": 1910104, "en": "BACKGROUND Over 70% of patients hospitalised in an intensive care unit (ICU) often experience moderate to severe pain due to pre-existing diseases, trauma, surgery, aggressive procedures, and routine ICU care. Many patients hospitalised in ICU are not able to speak and express their pain due to various causes, including mechanical ventilation, reduced consciousness, and administration of sedative drugs. Therefore, the use of observational and behavioural pain tools is recommended in this group of patients given their inability to express pain. AIM To examine the existing observational and behavioural tools for assessment of in Nonverbal Intubated Critically Adult Patients after Open-Heart Surgery. METHODS A systematic review of available observational and behavioural tools for assessment of pain was undertaken using the COSMIN checklist. A literature search was conducted using the following databases: Ovid, Science Direct, Scopus, PubMed, and CINHAL databases, Google Scholar search engine as well as Persian resources Sid, Magiran, Iran doc, and IranMedex up to the end of 2017 were reviewed. RESULTS A total of 47 studies that had examined five tools used in intensive care units after cardiac surgery in patients under mechanical ventilation were reviewed. Each of the five tools included behavioural and observational items, and only one tool had physiological items. All the tools had been evaluated regarding validity and reliability. In the three tools, sensitivity, specificity, responsiveness, and satisfaction were considered. CONCLUSION Based on available evidence and investigations, CPOT and BPS tools have good validity and reliability to be used in pain assessment in Nonverbal Intubated Critically Adult Patients after Open-Heart Surgery. The NVPS tool requires more studies to be further confirmed before the assessment of pain in this group of patients.", "gemini": "BỐI CẢNH Hơn 70% bệnh nhân nhập viện tại khoa chăm sóc đặc biệt (ICU) thường trải qua cơn đau từ trung bình đến dữ dội do các bệnh lý trước đó, chấn thương, phẫu thuật, các thủ thuật xâm lấn và chăm sóc ICU thường quy. Nhiều bệnh nhân nhập viện tại ICU không thể nói và bày tỏ cơn đau của mình do nhiều nguyên nhân, bao gồm thông khí cơ học, giảm ý thức và sử dụng thuốc an thần. Do đó, việc sử dụng các công cụ đánh giá đau theo quan sát và hành vi được khuyến cáo cho nhóm bệnh nhân này do họ không thể bày tỏ cơn đau. MỤC ĐÍCH Kiểm tra các công cụ quan sát và hành vi hiện có để đánh giá cơn đau ở Bệnh nhân người lớn nguy kịch không lời sau phẫu thuật tim hở. PHƯƠNG PHÁP Một đánh giá có hệ thống về các công cụ quan sát và hành vi có sẵn để đánh giá cơn đau đã được thực hiện bằng cách sử dụng danh sách kiểm tra COSMIN. Tìm kiếm tài liệu đã được thực hiện bằng cách sử dụng các cơ sở dữ liệu sau: Ovid, Science Direct, Scopus, PubMed và CINHAL, công cụ tìm kiếm của Google Scholar cũng như các nguồn tài nguyên Ba Tư Sid, Magiran, Iran doc và IranMedex cho đến hết năm 2017 đã được xem xét. KẾT QUẢ Tổng cộng 47 nghiên cứu đã kiểm tra năm công cụ được sử dụng trong các đơn vị chăm sóc đặc biệt sau phẫu thuật tim ở những bệnh nhân đang thở máy đã được xem xét. Mỗi công cụ trong số năm công cụ bao gồm các mục hành vi và quan sát và chỉ có một công cụ có các mục sinh lý. Tất cả các công cụ đã được đánh giá về độ hợp lệ và độ tin cậy. Trong ba công cụ, độ nhạy, độ đặc hiệu, độ đáp ứng và sự hài lòng đã được xem xét. KẾT LUẬN Dựa trên các bằng chứng và điều tra có sẵn, các công cụ CPOT và BPS có độ hợp lệ và độ tin cậy tốt để được sử dụng trong đánh giá cơn đau ở Bệnh nhân người lớn nguy kịch không lời sau phẫu thuật tim hở. Công cụ NVPS đòi hỏi nhiều nghiên cứu hơn để được xác nhận thêm trước khi đánh giá cơn đau ở nhóm bệnh nhân này."} +{"idx": 1910105, "en": "BACKGROUND Over the past two decades, more thorough investigations for hepatitis E virus (HEV) infection have been done in the world. Reports from Southeast European countries have increased. AIM The current article presents a critical analysis of all studies for HEV in Bulgaria. MATERIAL AND METHODS A literature search was done using available medical databases. We analysed the literature in PubMed databases and Bulgarian medical databases for English and Bulgarian languages sources. Preference was given to the sources published within the past 24 years (January 1995 - September 2018). RESULTS Two thousand two hundred and fifty-seven blood serums were tested for the analysed period (1995 - 2018), and 13.1% of them were positive for acute HEV (Mean ± SD: 20.38 ± 25.77%; 95% CI: 1.29 - 39.47%). The following subtypes were established in the country - HEV Subtype 3e, HEV Subtype 3f, HEV Subtype 3c, HEV Subtype 3i, HEV Subtype 3hi and HEV Subtype 1. CONCLUSION We hope that the National Health Organizations will take adequate and timely measures to increase the knowledge and research for HEV among Bulgarian citizens.", "gemini": "BỐI CẢNH Trong hai thập kỷ qua, nhiều cuộc điều tra toàn diện hơn về nhiễm virus viêm gan E (HEV) đã được thực hiện trên thế giới. Các báo cáo từ các nước Đông Nam Âu đã tăng lên. MỤC ĐÍCH Bài báo hiện tại trình bày một phân tích quan trọng về tất cả các nghiên cứu về HEV ở Bulgaria. VẬT LIỆU VÀ PHƯƠNG PHÁP Đã thực hiện tìm kiếm tài liệu bằng cách sử dụng các cơ sở dữ liệu y tế có sẵn. Chúng tôi đã phân tích các tài liệu trong cơ sở dữ liệu PubMed và cơ sở dữ liệu y tế của Bulgaria để tìm nguồn bằng tiếng Anh và tiếng Bulgaria. Ưu tiên các nguồn được công bố trong vòng 24 năm qua (tháng 1 năm 1995 - tháng 9 năm 2018). KẾT QUẢ Hai nghìn hai trăm năm mươi bảy huyết thanh máu đã được xét nghiệm trong giai đoạn được phân tích (1995 - 2018) và 13,1% trong số đó có kết quả dương tính với HEV cấp tính (Trung bình ± SD: 20,38 ± 25,77%; 95% CI: 1,29 - 39,47%). Các phân nhóm sau đã được thiết lập tại quốc gia này - Phân nhóm HEV 3e, Phân nhóm HEV 3f, Phân nhóm HEV 3c, Phân nhóm HEV 3i, Phân nhóm HEV 3hi và Phân nhóm HEV 1. KẾT LUẬN Chúng tôi hy vọng rằng các Tổ chức Y tế Quốc gia sẽ thực hiện các biện pháp kịp thời và đầy đủ để nâng cao kiến thức và nghiên cứu về HEV trong số những người dân Bulgaria."} +{"idx": 1910106, "en": "BACKGROUND Atrial fibrillation (AF) is one of the most common types of arrhythmia diagnosed in clinical practice. Due to its negative effects on people's physical and mental health, it is necessary to prevent and treat AF. Recently, scholars have found that acupuncture can be used to treat AF, but some scholars have questioned its therapeutic efficacy. AIM Therefore, this study was performed to assess the efficacy and safety of acupuncture treatment for AF patients. METHODS Previously published research articles were retrieved from six databases, and the data was analysed using RevMan5.3 software with a statistically significant difference defined as P < 0.05. RESULTS A total of 8 relevant kinds of literature were retrieved containing 633 AF patients (323 in the treatment group and 310 in the control group). Acupuncture treatment increased the total efficacy and the rate of AF cardioversion to sinus rhythm (RR: 1.38; 95% CI: 1.25 to 1.53 vs RR: 1.40;95% CI: 1.16 to 1.69; each P < 0.05), and decreased the time of AF cardioversion to sinus rhythm, the heart rate and incidence of adverse effects (RR: -3.95; 95% CI: -4.98 to -2.91 vs RR: -14.54; 95% CI: -24.09 to -5.00 vs RR: 0.48; 95% CI: 0.21 to 1.11, each P < 0.05). There was difference between retention time more and less than 30 minutes (I2 = 74.9%, P = 0.05). The funnel plot displayed a symmetrical and funnel-form shape, indicating low bias. CONCLUSION Acupuncture has a good therapeutic effect and safety profile on patients with AF, and its application in clinical practice should be considered.", "gemini": "BỐI CẢNH Rung nhĩ (AF) là một trong những loại loạn nhịp tim phổ biến nhất được chẩn đoán trong thực hành lâm sàng. Do những tác động tiêu cực của nó đến sức khỏe thể chất và tinh thần của mọi người, nên cần phải phòng ngừa và điều trị AF. Gần đây, các học giả đã phát hiện ra rằng châm cứu có thể được sử dụng để điều trị AF, nhưng một số học giả đã đặt câu hỏi về hiệu quả điều trị của nó. MỤC ĐÍCH Do đó, nghiên cứu này được thực hiện để đánh giá hiệu quả và độ an toàn của phương pháp điều trị châm cứu cho bệnh nhân AF. PHƯƠNG PHÁP Các bài báo nghiên cứu đã công bố trước đó đã được lấy từ sáu cơ sở dữ liệu và dữ liệu đã được phân tích bằng phần mềm RevMan5.3 với sự khác biệt có ý nghĩa thống kê được định nghĩa là P < 0,05. KẾT QUẢ Tổng cộng có 8 loại tài liệu có liên quan đã được lấy ra có chứa 633 bệnh nhân AF (323 trong nhóm điều trị và 310 trong nhóm đối chứng). Phương pháp điều trị châm cứu làm tăng hiệu quả tổng thể và tỷ lệ chuyển nhịp xoang của AF (RR: 1,38; 95% CI: 1,25 đến 1,53 so với RR: 1,40; 95% CI: 1,16 đến 1,69; mỗi P < 0,05), và giảm thời gian chuyển nhịp xoang của AF, nhịp tim và tỷ lệ tác dụng phụ (RR: -3,95; 95% CI: -4,98 đến -2,91 so với RR: -14,54; 95% CI: -24,09 đến -5,00 so với RR: 0,48; 95% CI: 0,21 đến 1,11, mỗi P < 0,05). Có sự khác biệt giữa thời gian duy trì nhiều hơn và ít hơn 30 phút (I2 = 74,9%, P = 0,05). Đồ thị phễu hiển thị hình dạng đối xứng và hình phễu, cho thấy độ lệch thấp. KẾT LUẬN Châm cứu có hiệu quả điều trị tốt và hồ sơ an toàn trên bệnh nhân AF, và nên xem xét ứng dụng của nó trong thực hành lâm sàng."} +{"idx": 1910107, "en": "OBJECTIVE The aim of this systematic review was to assess the transverse skeletal effects of rapid maxillary expansion (RME) in pre and post-pubertal subjects. MATERIAL AND METHODS Five databases were searched till May 2018; Pubmed, Cochrane, Scopus, Lilacs and Web of science in addition to the manual search of other sources. There were no language restrictions. Methodological Index for Non-Randomized Studies MINORS was used to assess the quality and risk of bias of the trials included. RESULTS Six studies were finally included in the qualitative analysis. A meta-analysis wasn't performed due to the heterogeneity of methodologies and outcomes. All of the included studies showed drawbacks in their structure yielding weak evidence. On the short term, RME caused an increase in the maxillary and lateral-nasal widths in pre-pubertal subjects by 3.4 mm and 3.3 mm, and by 2.8 and 2.2 mm respectively in post-pubertal subjects. Although statistically insignificant, the maxillary width increase was more than that of the post-pubertal subjects by 0.6 mm. Over the long term, expansion produced permanent increases in the transverse dimensions of both the dento-alveolar and skeletal components of the maxilla and circum-maxillary structures in pre-pubertal subjects. The post-pubertal subjects presented with a statistically significant increase only in the later-nasal width by 1.3 mm than the untreated controls with no permanent increase in the skeletal maxillary width. CONCLUSION The literature is very deficient regarding the use of skeletal age as a reference in the treatment of skeletal crossbites using RME. Only weak evidence exists supporting the increased maxillary and lateral-nasal widths after tooth-tissue borne RME in pre-pubertal subjects, with these effects being less in the post-pubertal ones.", "gemini": "MỤC TIÊU Mục đích của bài tổng quan có hệ thống này là để đánh giá các tác động của bộ xương ngang đối với việc mở rộng hàm trên nhanh (RME) ở những đối tượng trước và sau tuổi dậy thì. VẬT LIỆU VÀ PHƯƠNG PHÁP Đã tìm kiếm năm cơ sở dữ liệu cho đến tháng 5 năm 2018; Pubmed, Cochrane, Scopus, Lilacs và Web of science ngoài việc tìm kiếm thủ công các nguồn khác. Không có hạn chế về ngôn ngữ. Chỉ số phương pháp luận cho các nghiên cứu không ngẫu nhiên MINORS đã được sử dụng để đánh giá chất lượng và nguy cơ sai lệch của các thử nghiệm được đưa vào. KẾT QUẢ Cuối cùng có sáu nghiên cứu được đưa vào phân tích định tính. Không thực hiện phân tích tổng hợp do tính không đồng nhất của các phương pháp luận và kết quả. Tất cả các nghiên cứu được đưa vào đều cho thấy những nhược điểm trong cấu trúc của chúng dẫn đến bằng chứng yếu. Về mặt ngắn hạn, RME gây ra sự gia tăng chiều rộng hàm trên và mũi bên ở những đối tượng trước tuổi dậy thì là 3,4 mm và 3,3 mm, và lần lượt là 2,8 và 2,2 mm ở những đối tượng sau tuổi dậy thì. Mặc dù không có ý nghĩa thống kê, nhưng sự gia tăng chiều rộng hàm trên nhiều hơn so với những đối tượng sau tuổi dậy thì là 0,6 mm. Về lâu dài, việc mở rộng tạo ra sự gia tăng vĩnh viễn về kích thước ngang của cả thành phần răng-chân răng và bộ xương của xương hàm trên và các cấu trúc xung quanh xương hàm trên ở những đối tượng trước tuổi dậy thì. Những đối tượng sau tuổi dậy thì chỉ có sự gia tăng có ý nghĩa thống kê về chiều rộng mũi sau là 1,3 mm so với nhóm đối chứng không được điều trị mà không có sự gia tăng vĩnh viễn về chiều rộng xương hàm trên. KẾT LUẬN Tài liệu còn rất thiếu sót về việc sử dụng độ tuổi bộ xương làm tài liệu tham khảo trong điều trị các trường hợp cắn chéo xương bằng RME. Chỉ có bằng chứng yếu hỗ trợ sự gia tăng chiều rộng hàm trên và mũi bên sau khi RME mang mô răng ở những đối tượng trước tuổi dậy thì, với những tác động này ít hơn ở những đối tượng sau tuổi dậy thì."} +{"idx": 1910108, "en": "The gut microbiome is now considered as a large organ that has a direct effect on gastrointestinal tract, immune and endocrine system. There is no evidence that gut microbiota regulates the immune system and is responsible for bone formation and destruction. Probiotics have been shown through the gastrointestinal tract to have a positive effect on the management of the healthy bone. This article discusses the latest data available from PubMed and Scopus databases regarding gut microbiome, probiotics and bone briefly.", "gemini": "Hệ vi sinh đường ruột hiện được coi là một cơ quan lớn có tác động trực tiếp đến đường tiêu hóa, hệ miễn dịch và hệ nội tiết. Không có bằng chứng nào cho thấy hệ vi sinh đường ruột điều chỉnh hệ miễn dịch và chịu trách nhiệm cho quá trình hình thành và phá hủy xương. Probiotics đã được chứng minh qua đường tiêu hóa có tác dụng tích cực trong việc quản lý xương khỏe mạnh. Bài viết này thảo luận tóm tắt về dữ liệu mới nhất có sẵn từ các cơ sở dữ liệu PubMed và Scopus liên quan đến hệ vi sinh đường ruột, probiotics và xương."} +{"idx": 1910109, "en": "Phytoestrogens have structures that are very similar to endogen estradiol derived from plants. Phytoestrogens sources are widespread in Asian regions including in Indonesia. Its have health benefits including reproductive health, heart health, a role in weight loss, hormone-dependent tumours, bone and skin health, and the immune system. This review aimed to provide information on phytoestrogens' effect on human health.", "gemini": "Phytoestrogen có cấu trúc rất giống với estradiol nội sinh có nguồn gốc từ thực vật. Các nguồn phytoestrogen rất phổ biến ở các khu vực châu Á bao gồm cả Indonesia. Nó có lợi cho sức khỏe bao gồm sức khỏe sinh sản, sức khỏe tim mạch, vai trò trong việc giảm cân, khối u phụ thuộc hormone, sức khỏe xương và da cũng như hệ miễn dịch. Mục đích của bài đánh giá này là cung cấp thông tin về tác dụng của phytoestrogen đối với sức khỏe con người."} +{"idx": 1910110, "en": "Among the first physicians and physiologists at the pre-Hippocratic medicine with contradictions and oscillating doctrines was Alcmaeon from Croton in the 6th century BC. For many, he shared as the father of scientific medicine. Unfortunately, we have only eighteen texts written for him and only five fragments of texts written by him. This saved extracts and testimonies they refer mostly to physiology, epistemology and psychology. Was born in the city of Croton (Κρτων) in Magna Graecia (southern Italy) was a reference point for the activity of physicians and philosophers over the years. Furthermore, Herodotus tells us about the excellent practice quality of the physicians, \"compared to all the others known in that period\", among which Alcmaeon. But Croton is also famous as the centre of thought and activity of the philosopher Pythagoras of Samos (Πυθαγρας ὁ Σμιος, 580-500 BC) through his Pythagorean school around 530 BC.", "gemini": "Một trong những bác sĩ và nhà sinh lý học đầu tiên trong y học tiền Hippocrates với những mâu thuẫn và học thuyết dao động là Alcmaeon đến từ Croton vào thế kỷ thứ 6 trước Công nguyên. Đối với nhiều người, ông được coi là cha đẻ của y học khoa học. Thật không may, chúng ta chỉ có mười tám văn bản do ông viết và chỉ có năm đoạn trích văn bản do ông viết. Những trích dẫn và lời chứng được lưu lại này chủ yếu đề cập đến sinh lý học, nhận thức luận và tâm lý học. Sinh ra tại thành phố Croton (Κρτων) ở Magna Graecia (miền nam nước Ý) là điểm tham chiếu cho hoạt động của các bác sĩ và nhà triết học trong nhiều năm. Hơn nữa, Herodotus kể cho chúng ta về chất lượng thực hành tuyệt vời của các bác sĩ, \"so với tất cả những bác sĩ khác được biết đến trong thời kỳ đó\", trong số đó có Alcmaeon. Nhưng Croton cũng nổi tiếng là trung tâm tư tưởng và hoạt động của nhà triết học Pythagoras xứ Samos (Πυθαγρας ὁ Σμιος, 580-500 trước Công nguyên) thông qua trường phái Pythagore của ông vào khoảng năm 530 trước Công nguyên."} +{"idx": 1910111, "en": "One step melanoma surgery (OSMS) is applicable to all patients with clear clinical and dermatoscopic criteria for thin cutaneous melanomas or melanoma in situ lesions, even without the need for preoperative tumour thickness measurement. Amelanotic melanomas and melanomas with clinical and dermatoscopical features for regression zones could be problematic when applying the OSMS. The methodology could be also applicable to all groups of patients where the tumour thickness could be measured preoperatively/by ultrasound (while in parallel also determining the status of the locoregional lymph nodes). For tumours with a tumour thickness between 2 and 4 mm, but also over 4mm the OSMS seems to be the correct choice.", "gemini": "Phẫu thuật cắt bỏ u hắc tố một bước (OSMS) có thể áp dụng cho tất cả bệnh nhân có các tiêu chuẩn lâm sàng và soi da rõ ràng đối với các khối u hắc tố da mỏng hoặc các tổn thương u hắc tố tại chỗ, ngay cả khi không cần đo độ dày khối u trước phẫu thuật. U hắc tố không sắc tố và u hắc tố có các đặc điểm lâm sàng và soi da đối với các vùng thoái triển có thể gây ra vấn đề khi áp dụng OSMS. Phương pháp này cũng có thể áp dụng cho tất cả các nhóm bệnh nhân mà độ dày khối u có thể được đo trước phẫu thuật/bằng siêu âm (trong khi song song cũng xác định tình trạng của các hạch bạch huyết cục bộ). Đối với các khối u có độ dày khối u từ 2 đến 4 mm, nhưng cũng trên 4 mm, OSMS dường như là lựa chọn đúng."} +{"idx": 1910112, "en": "Background Hepatitis C virus (HCV) infection has a high rate of chronicity, attributable to its capacity to alter host immunity, including natural killer (NK) cell function. In this study, the interaction between NK cell activity and HCV viral load was investigated. Methods Peripheral blood NK cells were examined for cytotoxicity and interferon (IFN)-γ expression in HCV infected low (LVL, < 800,000 IU/mL, n = 10) and high (HVL, > 800,000 IU/mL, n = 13) viral load patient cohorts. Results Spontaneous NK cell cytotoxicity was more robust in the LVL cohort resulting in a negative correlation with viral loads (spontaneous, r = -0.437, P = 0.037; IFN-α activated, r = -0.372, P = 0.081). Although the percent of IFN-γ+ NK cells did not associate with viral load, within the LVL cohort there was a marked increase in IFN-γ+ NK cells upon IFN-α activation relative to medium alone (P < 0.01). To examine the inability of NK cells derived from HVL patients to be further activated, the expression of the exhaustion marker programmed cell death protein (PD)-1 was evaluated. PD-1 expression upon NK cell activation correlated with viral load (r = 0.649, P = 0.009). In addition, HCV proteins upregulated PD-1 expression in vitro (P < 0.05), suggesting that HCV can directly promote NK cell exhaustion. Cells from HVL patients were also more likely to produce IFN-γ in response to HCV core protein. The finding that NK cell PD-1 and IFN-γ expression are linked (r = 0.542, P < 0.05) suggests that increased IFN-γ levels may induce PD-1 as a negative feedback mechanism. Conclusions High HCV loads appear to promote NK exhaustion in chronic HCV infection.", "gemini": "Bối cảnh Nhiễm virus viêm gan C (HCV) có tỷ lệ mãn tính cao, do khả năng làm thay đổi khả năng miễn dịch của vật chủ, bao gồm chức năng của tế bào giết tự nhiên (NK). Trong nghiên cứu này, tương tác giữa hoạt động của tế bào NK và tải lượng virus HCV đã được điều tra. Phương pháp Các tế bào NK máu ngoại vi đã được kiểm tra về độc tính tế bào và biểu hiện interferon (IFN) -γ ở những bệnh nhân nhiễm HCV có tải lượng virus thấp (LVL, <800.000 IU/mL, n = 10) và cao (HVL, > 800.000 IU/mL, n = 13). Kết quả Độc tính tế bào NK tự phát mạnh hơn ở nhóm LVL dẫn đến tương quan âm với tải lượng virus (tự phát, r = -0,437, P = 0,037; hoạt hóa IFN-α, r = -0,372, P = 0,081). Mặc dù tỷ lệ tế bào NK IFN-γ + không liên quan đến tải lượng virus, nhưng trong nhóm LVL có sự gia tăng đáng kể các tế bào NK IFN-γ + khi hoạt hóa IFN-α so với môi trường đơn thuần (P <0,01). Để kiểm tra khả năng không thể kích hoạt thêm các tế bào NK có nguồn gốc từ bệnh nhân HVL, biểu hiện của protein chết tế bào theo chương trình đánh dấu kiệt sức (PD) -1 đã được đánh giá. Biểu hiện PD-1 khi hoạt hóa tế bào NK tương quan với tải lượng virus (r = 0,649, P = 0,009). Ngoài ra, các protein HCV còn điều chỉnh tăng biểu hiện PD-1 trong ống nghiệm (P <0,05), cho thấy rằng HCV có thể thúc đẩy trực tiếp sự kiệt sức của tế bào NK. Các tế bào từ bệnh nhân HVL cũng có nhiều khả năng sản xuất IFN-γ để đáp ứng với protein lõi HCV. Phát hiện rằng biểu hiện PD-1 và IFN-γ của tế bào NK có liên quan (r = 0,542, P <0,05) cho thấy rằng nồng độ IFN-γ tăng có thể gây ra PD-1 như một cơ chế phản hồi tiêu cực. Kết luận Tải lượng HCV cao dường như thúc đẩy sự kiệt sức của NK trong nhiễm HCV mãn tính."} +{"idx": 1910113, "en": "Background Hepatocellular carcinoma (HCC) has a poor prognosis with low chemotherapeutic efficiency to medications except to sorafenib. Previous studies showed that adverse events (AEs) of sorafenib can predict therapy efficacy to HCC. The aim of the study is to evaluate the early efficacy and AEs of sorafenib therapy. Methods The database of HCC patients receiving sorafenib at Taichung Veterans General Hospital during the period from June 2012 to October 2016 was analyzed. All HCC cases were Barcelona Clinic Liver Cancer (BCLC) classification stage C. The early efficacy of sorafenib was classified according to the mRECIST criteria as either partial response (PR), stable disease (SD) or progressive disease (PD). Responses were recorded within 6 weeks after the start of sorafenib treatment. AEs were defined as the appearance of hand-foot skin reaction (HFSR), hypertension (HTN) and diarrhea. Exclusion criteria were poor performance status, poor drug compliance, discontinued follow-up or mortality occurring within 1 day after medication. Results From a total of 222 subjects, eight cases (3.6%) were classified as PR, 82 cases (36.9%) SD, and 132 cases (59.5%) PD. The PR group had the highest ratio of HFSR (62.4%) and hypertension (37.5%). Pooling cases of PR and SD together, the presence of HFSR adjusted odd ratio (aOR) 2.80, 95% confidence interval (CI) 1.52 - 5.16) and diarrhea (aOR 3.42, 95% CI 1.67 - 7.01) were good predictors of favorable responses to sorafenib therapy. Conclusions HFSR and diarrhea are good predictors of early therapy efficacy to the sorafenib treatment.", "gemini": "Bối cảnh Ung thư biểu mô tế bào gan (HCC) có tiên lượng kém với hiệu quả hóa trị thấp đối với các loại thuốc ngoại trừ sorafenib. Các nghiên cứu trước đây cho thấy các tác dụng phụ (AE) của sorafenib có thể dự đoán hiệu quả điều trị đối với HCC. Mục đích của nghiên cứu là đánh giá hiệu quả sớm và AE của liệu pháp sorafenib. Phương pháp Cơ sở dữ liệu của bệnh nhân HCC dùng sorafenib tại Bệnh viện Đa khoa Cựu chiến binh Đài Trung trong giai đoạn từ tháng 6 năm 2012 đến tháng 10 năm 2016 đã được phân tích. Tất cả các trường hợp HCC đều được phân loại giai đoạn C theo phân loại ung thư gan của Bệnh viện Clinic Barcelona (BCLC). Hiệu quả sớm của sorafenib được phân loại theo tiêu chuẩn mRECIST là đáp ứng một phần (PR), bệnh ổn định (SD) hoặc bệnh tiến triển (PD). Các phản ứng được ghi nhận trong vòng 6 tuần sau khi bắt đầu điều trị sorafenib. AE được định nghĩa là sự xuất hiện của phản ứng da bàn tay-bàn chân (HFSR), tăng huyết áp (HTN) và tiêu chảy. Tiêu chuẩn loại trừ là tình trạng hoạt động kém, tuân thủ thuốc kém, ngừng theo dõi hoặc tử vong xảy ra trong vòng 1 ngày sau khi dùng thuốc. Kết quả Trong tổng số 222 đối tượng, có tám trường hợp (3,6%) được phân loại là PR, 82 trường hợp (36,9%) SD và 132 trường hợp (59,5%) PD. Nhóm PR có tỷ lệ HFSR cao nhất (62,4%) và tăng huyết áp (37,5%). Gộp các trường hợp PR và SD lại với nhau, tỷ lệ chênh lệch điều chỉnh HFSR (aOR) 2,80, khoảng tin cậy 95% (CI) 1,52 - 5,16) và tiêu chảy (aOR 3,42, 95% CI 1,67 - 7,01) là những yếu tố dự báo tốt về đáp ứng thuận lợi với liệu pháp sorafenib. Kết luận HFSR và tiêu chảy là những yếu tố dự báo tốt về hiệu quả điều trị sớm đối với điều trị sorafenib."} +{"idx": 1910114, "en": "Background Acute lower gastrointestinal bleeding (ALGIB) can occur in patients on anticoagulant therapy (either warfarin or non-vitamin K oral anticoagulants (NOACs)). Use of NOACs has been increasing compared to warfarin in recent years. We analyzed patients with ALGIB on anticoagulation therapy and compared characteristics, management and clinical outcome in patients treated with NOACs versus warfarin. Methods All patients with ALGIB on anticoagulation therapy treated in two (affiliated) centers during a 7-year period were evaluated. Characteristics and clinical outcome were compared between patients on warfarin and patients on NOACs. Results Out of the 587 patients identified with ALGIB during the study period, 43 (7.3%) were on NOACs and 68 (11.6%) on warfarin. Mean age was 75.9 ± 9.5 and 77.1 ± 7.9 years respectively. Site of bleeding was located in the small bowel in 2/43 of NOAC patients and 6/68 of warfarin group. Vascular ectasias (8/43 vs. 6/68, P = 0.010) and polyps/neoplasia (13/43 vs. 6/68, P = 0.025) were more commonly causes of bleeding in patients on NOACs. While endoscopic hemostasis was more commonly needed in patients on NOACs (17/43 vs. 14/68, P = 0.049), they required less hospitalization days (4.5 ± 3.6 vs. 6.1 ± 4.2, P = 0.032). Blood transfusions and need for other interventions (embolization and/or surgery) as well as recurrence of bleeding and mortality were not statistically different. Conclusions Although NOAC patients with ALGIB exhibit some differences on certain clinical characteristics when compared to warfarin patients, they share a similar clinical outcome.", "gemini": "Bối cảnh Xuất huyết tiêu hóa dưới cấp tính (ALGIB) có thể xảy ra ở những bệnh nhân đang điều trị bằng thuốc chống đông (warfarin hoặc thuốc chống đông đường uống không phải vitamin K (NOAC)). Việc sử dụng NOAC đã tăng so với warfarin trong những năm gần đây. Chúng tôi đã phân tích những bệnh nhân bị ALGIB khi đang điều trị bằng thuốc chống đông và so sánh các đặc điểm, cách xử trí và kết cục lâm sàng ở những bệnh nhân được điều trị bằng NOAC so với warfarin. Phương pháp Tất cả các bệnh nhân bị ALGIB khi đang điều trị bằng thuốc chống đông được điều trị tại hai trung tâm (có liên kết) trong giai đoạn 7 năm đã được đánh giá. Các đặc điểm và kết cục lâm sàng đã được so sánh giữa những bệnh nhân dùng warfarin và những bệnh nhân dùng NOAC. Kết quả Trong số 587 bệnh nhân được xác định bị ALGIB trong thời gian nghiên cứu, 43 (7,3%) bệnh nhân dùng NOAC và 68 (11,6%) bệnh nhân dùng warfarin. Độ tuổi trung bình lần lượt là 75,9 ± 9,5 và 77,1 ± 7,9. Vị trí chảy máu nằm ở ruột non ở 2/43 bệnh nhân dùng NOAC và 6/68 bệnh nhân dùng warfarin. Giãn mạch (8/43 so với 6/68, P = 0,010) và polyp/u tân sinh (13/43 so với 6/68, P = 0,025) thường là nguyên nhân gây chảy máu ở những bệnh nhân dùng NOAC. Trong khi cầm máu nội soi thường cần thiết hơn ở những bệnh nhân dùng NOAC (17/43 so với 14/68, P = 0,049), thì họ cần ít ngày nằm viện hơn (4,5 ± 3,6 so với 6,1 ± 4,2, P = 0,032). Truyền máu và nhu cầu can thiệp khác (thuyên tắc và/hoặc phẫu thuật) cũng như tái phát chảy máu và tử vong không khác biệt về mặt thống kê. Kết luận Mặc dù bệnh nhân dùng NOAC bị ALGIB biểu hiện một số khác biệt về một số đặc điểm lâm sàng khi so sánh với bệnh nhân dùng warfarin, nhưng họ có kết cục lâm sàng tương tự."} +{"idx": 1910115, "en": "Background The low fermentable oligosaccharide, disaccharide, monosaccharide and polyol (FODMAP) diet causes significant clinical improvement in patients with irritable bowel syndrome (IBS). Joint hypermobility syndrome (JHS), defined as musculoskeletal symptoms in a hypermobile individual in the absence of systemic rheumatological disease, may be associated with functional gastrointestinal symptoms, including IBS. The aim of this study is to examine whether JHS can affect the response to the low FODMAP diet in patients with IBS. Methods In this retrospective study, we included patients with IBS according to Rome III criteria who had followed a low FODMAP diet. Symptoms scores were measured before and after the low FODMAP diet. Results A total of 165 patients (130 females, age 44 ± 14 years) were included. Diarrhea predominant IBS (IBS-D) was present in 40.6% of our patients while JHS was present in 21.2%. The score for abdominal pain was higher for JHS compared to non-JHS prior to intervention (P = 0.011). Symptoms improved in both groups of patients after a low FODMAP diet (P < 0.0001). The largest effects were shown with significant decreases of the average score and bloating. When broken down by JHS and IBS type, a low FODMAP diet significantly improved pain, bloating, diarrhea, constipation, and the average score with the largest effect in JHS/constipation predominant IBS (IBS-C), JHS/mixed IBS and unclassified IBS (IBS-M), JHS/IBS-D, non-JHS/IBS-C and JHS/IBS-M, respectively. Conclusions Our study suggests that a low FODMAP diet has a greater effect on IBS symptoms in JHS than non-JHS patients.", "gemini": "Bối cảnh Chế độ ăn ít oligosaccharide, disaccharide, monosaccharide và polyol lên men (FODMAP) cải thiện đáng kể tình trạng lâm sàng ở những bệnh nhân bị hội chứng ruột kích thích (IBS). Hội chứng tăng vận động khớp (JHS), được định nghĩa là các triệu chứng cơ xương ở một cá thể tăng vận động trong trường hợp không có bệnh thấp khớp hệ thống, có thể liên quan đến các triệu chứng tiêu hóa chức năng, bao gồm IBS. Mục đích của nghiên cứu này là kiểm tra xem JHS có thể ảnh hưởng đến đáp ứng với chế độ ăn ít FODMAP ở những bệnh nhân bị IBS hay không. Phương pháp Trong nghiên cứu hồi cứu này, chúng tôi đã đưa vào những bệnh nhân bị IBS theo tiêu chuẩn Rome III đã tuân theo chế độ ăn ít FODMAP. Điểm triệu chứng được đo trước và sau chế độ ăn ít FODMAP. Kết quả Tổng cộng có 165 bệnh nhân (130 nữ, tuổi 44 ± 14) được đưa vào. IBS tiêu chảy chiếm ưu thế (IBS-D) có ở 40,6% bệnh nhân của chúng tôi trong khi JHS có ở 21,2%. Điểm đau bụng cao hơn ở JHS so với không phải JHS trước khi can thiệp (P = 0,011). Các triệu chứng cải thiện ở cả hai nhóm bệnh nhân sau chế độ ăn ít FODMAP (P < 0,0001). Các tác dụng lớn nhất được thể hiện với sự giảm đáng kể về điểm trung bình và đầy hơi. Khi phân tích theo loại JHS và IBS, chế độ ăn ít FODMAP cải thiện đáng kể tình trạng đau, đầy hơi, tiêu chảy, táo bón và điểm trung bình với tác dụng lớn nhất ở JHS/IBS táo bón chiếm ưu thế (IBS-C), JHS/IBS hỗn hợp và IBS chưa phân loại (IBS-M), JHS/IBS-D, không phải JHS/IBS-C và JHS/IBS-M, tương ứng. Kết luận Nghiên cứu của chúng tôi cho thấy chế độ ăn ít FODMAP có tác dụng lớn hơn đối với các triệu chứng IBS ở bệnh nhân JHS so với bệnh nhân không phải JHS."} +{"idx": 1910116, "en": "Endometrial cancer is the commonest gynecologic malignancy, of which adenocarcinoma is the most common histologic type. While most women who relapse present with local symptoms within 3 years of the initial diagnosis, metastatic disease usually involves the abdominal cavity and liver. Herein we present a rare case of a patient with a remote history of endometrial adenocarcinoma status-post curative surgical resection and adjuvant therapy who presented with obstructive jaundice proved to be secondary to peri-ampullary metastasis of uterine adenocarcinoma.", "gemini": "Ung thư nội mạc tử cung là bệnh ác tính phụ khoa phổ biến nhất, trong đó ung thư tuyến là loại mô học phổ biến nhất. Trong khi hầu hết phụ nữ tái phát có các triệu chứng tại chỗ trong vòng 3 năm kể từ khi chẩn đoán ban đầu, bệnh di căn thường liên quan đến ổ bụng và gan. Ở đây chúng tôi trình bày một trường hợp hiếm gặp về một bệnh nhân có tiền sử xa về ung thư tuyến nội mạc tử cung sau phẫu thuật cắt bỏ u lành tính và liệu pháp bổ trợ đã xuất hiện tình trạng vàng da tắc nghẽn được chứng minh là thứ phát do di căn quanh ống dẫn của ung thư tuyến tử cung."} +{"idx": 1910117, "en": "Peutz-Jeghers syndrome (PJS) is a rare autosomal dominant disorder characterized by hamartomatous polyps throughout the gastrointestinal tract, mostly causing gastrointestinal bleeding and recurrent intestinal obstructions. Few intussusception related to PJS occurs reportedly in adults. Unlike pediatric cases almost all reported cases end up with surgical resection. Here we present a case of PSJ-related intussusception in an adult patient treated successfully with endoscopic approach, with no surgical intervention required.", "gemini": "Hội chứng Peutz-Jeghers (PJS) là một rối loạn trội trên nhiễm sắc thể thường hiếm gặp có đặc điểm là các polyp dạng u hamartomatous ở toàn bộ đường tiêu hóa, chủ yếu gây chảy máu đường tiêu hóa và tắc ruột tái phát. Có ít trường hợp lồng ruột liên quan đến PJS được báo cáo ở người lớn. Không giống như các trường hợp nhi khoa, hầu hết các trường hợp được báo cáo đều phải cắt bỏ bằng phẫu thuật. Ở đây chúng tôi trình bày một trường hợp lồng ruột liên quan đến PSJ ở một bệnh nhân trưởng thành được điều trị thành công bằng phương pháp nội soi, không cần can thiệp phẫu thuật."} +{"idx": 1910118, "en": "Malignant mesothelioma is a rare neoplasm of the serosal linings. Mesothelioma has been linked to asbestos exposure, with prior asbestos exposure linked to 33-50% of malignant peritoneal mesotheliomas. We describe a case of malignant peritoneal mesothelioma (MPM) without any prior exposure to asbestos in a 40-year-old Hispanic female who presented to the emergency department with worsening abdominal pain and distension. She had a history of beta thalassemia trait and iron deficiency anemia. Examination revealed a distended abdomen with protruding umbilicus and positive shifting dullness. Laboratory tests showed anemia. Computed tomography (CT) of the abdomen revealed massive complex ascites suspicious of a malignant process. Ascitic fluid analysis showed serum ascites albumin gradient (SAAG) of 1.1 g/dL with a total protein of 5.2 g/dL. She underwent laparoscopic peritoneal biopsy which yielded epithelioid type malignant mesothelioma. She was started on chemotherapy with cisplatin and pemetrexed. The last follow-up was 27 months after the diagnosis. MPM is a rare and life-threatening malignancy. Frequently, the symptoms are non-specific. This poses a diagnostic challenge for physicians and probably the reason why the diagnosis is often delayed, especially in the absence of risk factors.", "gemini": "Trung biểu mô ác tính là một khối u tân sinh hiếm gặp của lớp lót thanh mạc. Trung biểu mô liên quan đến phơi nhiễm amiăng, với phơi nhiễm amiăng trước đó liên quan đến 33-50% các trường hợp trung biểu mô phúc mạc ác tính. Chúng tôi mô tả một trường hợp trung biểu mô phúc mạc ác tính (MPM) mà không có bất kỳ phơi nhiễm amiăng nào trước đó ở một phụ nữ gốc Tây Ban Nha 40 tuổi, người đến khoa cấp cứu vì đau bụng và chướng bụng ngày càng nặng. Cô ấy có tiền sử đặc điểm thalassemia beta và thiếu máu do thiếu sắt. Khám lâm sàng thấy bụng chướng, rốn lồi và có độ xỉn di động. Xét nghiệm trong phòng thí nghiệm cho thấy thiếu máu. Chụp cắt lớp vi tính (CT) ổ bụng cho thấy cổ trướng phức tạp lớn nghi ngờ là một quá trình ác tính. Phân tích dịch cổ trướng cho thấy chênh lệch albumin cổ trướng huyết thanh (SAAG) là 1,1 g/dL với tổng lượng protein là 5,2 g/dL. Cô đã trải qua sinh thiết phúc mạc nội soi và cho kết quả là trung biểu mô ác tính loại biểu mô. Cô ấy đã bắt đầu hóa trị bằng cisplatin và pemetrexed. Lần theo dõi cuối cùng là 27 tháng sau khi chẩn đoán. MPM là một bệnh ác tính hiếm gặp và đe dọa tính mạng. Các triệu chứng thường không đặc hiệu. Điều này đặt ra thách thức chẩn đoán cho các bác sĩ và có lẽ là lý do tại sao chẩn đoán thường bị trì hoãn, đặc biệt là khi không có các yếu tố nguy cơ."} +{"idx": 1910119, "en": "Background We aim to externally validate the status of emergency department (ED) appropriate utilization and 72-h ED returns among homeless patients. Methods This is a retrospective single-center observational study. Patients were divided into two groups (homeless versus non-homeless). Patients' general characteristics, clinical variables, ED appropriate utilization, and ED return disposition deviations were compared and analyzed separately. Results Study enrolled a total of 63,990 ED visits. Homeless patients comprised 9.3% (5,926) of visits. Higher ED 72-h returns occurred among homeless patients in comparison to the non-homeless patients (17% versus 5%, P < 0.001). Rate of significant ED disposition deviations (e.g., admission, triage to operation room, or death) on return visits were lower in homeless patients when compared to non-homeless patient populations (15% versus 23%, P < 0.001). Conclusions Though ED return rate was higher among homeless patients, return visit case management seems appropriate, indicating that 72-h ED returns might not be an optimal healthcare quality measurement for homeless patients.", "gemini": "Bối cảnh Chúng tôi đặt mục tiêu xác nhận bên ngoài tình trạng sử dụng hợp lý của khoa cấp cứu (ED) và tỷ lệ quay lại ED trong vòng 72 giờ ở những bệnh nhân vô gia cư. Phương pháp Đây là một nghiên cứu quan sát hồi cứu tại một trung tâm. Bệnh nhân được chia thành hai nhóm (vô gia cư so với không vô gia cư). Các đặc điểm chung của bệnh nhân, các biến lâm sàng, việc sử dụng hợp lý của ED và các độ lệch về tình trạng quay lại ED đã được so sánh và phân tích riêng biệt. Kết quả Nghiên cứu đã ghi danh tổng cộng 63.990 lượt khám cấp cứu. Bệnh nhân vô gia cư chiếm 9,3% (5.926) lượt khám. Tỷ lệ quay lại ED trong vòng 72 giờ cao hơn ở những bệnh nhân vô gia cư so với những bệnh nhân không vô gia cư (17% so với 5%, P < 0,001). Tỷ lệ lệch hướng xử trí đáng kể của ED (ví dụ: nhập viện, phân loại vào phòng phẫu thuật hoặc tử vong) khi tái khám thấp hơn ở bệnh nhân vô gia cư khi so sánh với quần thể bệnh nhân không vô gia cư (15% so với 23%, P < 0,001). Kết luận Mặc dù tỷ lệ quay lại ED cao hơn ở những bệnh nhân vô gia cư, nhưng việc quản lý trường hợp tái khám có vẻ phù hợp, cho thấy việc quay lại ED trong vòng 72 giờ có thể không phải là phép đo chất lượng chăm sóc sức khỏe tối ưu cho những bệnh nhân vô gia cư."} +{"idx": 1910120, "en": "Background There are several researches demonstrating that community-based educational and exercise programs can improve oral function in older people. However, the relationship between oral function and long-term participation in health programs has not been fully elucidated. The objective of this study was to clarify the oral health status and oral function of older people (≥ 65 years) who had been participating in community-based exercise programs at community salons. Methods We enrolled 108 women (mean age: 77.6 ± 5.7 years) who participated in oral and physical exercise programs once a week at community salons in Takehara throughout May 2017. This cross-sectional pilot study was approved by the Ethical Committee of Hiroshima University and informed consent was obtained from all participants. To assess oral function, the following tests were performed: tongue pressure test, oral diadochokinesis (ODK) measurement, repetitive saliva swallowing test (RSST) and oral wetness measurement. Results A significant negative correlation was found between age and ODK or RSST (Spearman's rank correlation; P = 0.007 and P = 0.01, respectively). The duration of participation ranged widely from 1 month to 7 years (median: 2.3 years). Although there was no significant difference between participation period and oral function, individuals who had been participating for 3 years or longer did not demonstrate a negative relationship between age and oral wetness. Furthermore, they exhibited a weaker negative relationship between age and oral function (i.e. tongue pressure, ODK and RSST) when compared with individuals who had been participating for less than 3 years. Conclusions Our results suggest that long-term participation in community salon exercise programs may suppress the deterioration of oral function in older people. Further study will be necessary to clarify the significant correlation between oral function and community-based social activities such as oral exercise.", "gemini": "Bối cảnh Có một số nghiên cứu chứng minh rằng các chương trình tập thể dục và giáo dục cộng đồng có thể cải thiện chức năng răng miệng ở người cao tuổi. Tuy nhiên, mối quan hệ giữa chức năng răng miệng và sự tham gia dài hạn vào các chương trình sức khỏe vẫn chưa được làm sáng tỏ đầy đủ. Mục tiêu của nghiên cứu này là làm rõ tình trạng sức khỏe răng miệng và chức năng răng miệng của người cao tuổi (≥ 65 tuổi) đã tham gia các chương trình tập thể dục cộng đồng tại các phòng tập cộng đồng. Phương pháp Chúng tôi đã tuyển dụng 108 phụ nữ (tuổi trung bình: 77,6 ± 5,7 tuổi) tham gia các chương trình tập thể dục răng miệng và thể chất một lần một tuần tại các phòng tập cộng đồng ở Takehara trong suốt tháng 5 năm 2017. Nghiên cứu thí điểm cắt ngang này đã được Ủy ban Đạo đức của Đại học Hiroshima chấp thuận và đã có được sự đồng ý của tất cả những người tham gia. Để đánh giá chức năng răng miệng, chúng tôi đã thực hiện các xét nghiệm sau: xét nghiệm áp lực lưỡi, đo vận động phối hợp miệng (ODK), xét nghiệm nuốt nước bọt lặp lại (RSST) và đo độ ướt của miệng. Kết quả Có một mối tương quan âm đáng kể giữa tuổi tác và ODK hoặc RSST (hệ số tương quan bậc của Spearman; P = 0,007 và P = 0,01, tương ứng). Thời gian tham gia dao động rộng từ 1 tháng đến 7 năm (trung vị: 2,3 năm). Mặc dù không có sự khác biệt đáng kể giữa thời gian tham gia và chức năng răng miệng, nhưng những người đã tham gia trong 3 năm trở lên không biểu hiện mối quan hệ tiêu cực giữa tuổi tác và độ ướt của miệng. Hơn nữa, họ biểu hiện mối quan hệ âm yếu hơn giữa tuổi tác và chức năng răng miệng (tức là áp lực lưỡi, ODK và RSST) khi so sánh với những người đã tham gia dưới 3 năm. Kết luận Kết quả của chúng tôi cho thấy rằng việc tham gia lâu dài vào các chương trình tập thể dục tại phòng tập cộng đồng có thể ngăn chặn sự suy giảm chức năng răng miệng ở người cao tuổi. Cần nghiên cứu thêm để làm rõ mối tương quan đáng kể giữa chức năng răng miệng và các hoạt động xã hội dựa vào cộng đồng như tập thể dục răng miệng."} +{"idx": 1910121, "en": "Background Misdiagnosis of the severity of acute appendicitis may lead to perforation and can consequently result in increased morbidity and mortality. In this study, the role of hyperbilirubinemia as a predictor of perforation is assessed by performing a meta-analysis of diagnostic accuracy. Methods A systematic search of the literature published over the past 20 years was performed using the EMBASE, PubMed, Cochrane library, and Google Scholar databases. Results Low values of sensitivity, specificity, and diagnostic odds ratio (DOR) were detected: 0.21 (95% confidence interval (CI): 0.13 - 0.30, standard error (SE) = 0.43), 0.27 (95% CI: 0.15 - 0.43, SE = 0.73), and 0.10 (95% CI: 0.3 - 0.28, SE = 0.05), respectively. The positive likelihood ratio (PLR) was low (0.29 (95% CI: 0.27 - 0.91, SE = 0.76)), whereas the negative likelihood ratio (NLR) was high (2.88 (95% CI: 1.66 - 5.14, SE = 0.10)). The hierarchical summary receiver operating characteristic curve was positioned towards the lower right corner, and the area under the curve was 0.19, both indicating a low level of overall accuracy and discrimination. Compared with the PLR, the negative inverse likelihood ratio (1/LR-) indicated that a positive result has a greater impact on the odds of disease than does a negative result. Conclusions Hyperbilirubinemia alone is not a reliable tool to predict perforation. Future studies should investigate whether the combined predictive values of bilirubin, C-reactive protein (CRP), and white blood cells are a more effective diagnostic tool.", "gemini": "Bối cảnh Chẩn đoán sai mức độ nghiêm trọng của viêm ruột thừa cấp tính có thể dẫn đến thủng và do đó có thể dẫn đến tăng tỷ lệ mắc bệnh và tử vong. Trong nghiên cứu này, vai trò của tăng bilirubin máu như một yếu tố dự báo thủng được đánh giá bằng cách thực hiện phân tích tổng hợp về độ chính xác của chẩn đoán. Phương pháp Một tìm kiếm có hệ thống các tài liệu được công bố trong 20 năm qua đã được thực hiện bằng cách sử dụng các cơ sở dữ liệu EMBASE, PubMed, thư viện Cochrane và Google Scholar. Kết quả Các giá trị thấp về độ nhạy, độ đặc hiệu và tỷ lệ chẩn đoán (DOR) đã được phát hiện: 0,21 (khoảng tin cậy 95% (CI): 0,13 - 0,30, sai số chuẩn (SE) = 0,43), 0,27 (95% CI: 0,15 - 0,43, SE = 0,73) và 0,10 (95% CI: 0,3 - 0,28, SE = 0,05), tương ứng. Tỷ lệ khả năng dương tính (PLR) thấp (0,29 (95% CI: 0,27 - 0,91, SE = 0,76)), trong khi tỷ lệ khả năng âm tính (NLR) cao (2,88 (95% CI: 1,66 - 5,14, SE = 0,10)). Đường cong đặc tính hoạt động của người nhận tóm tắt phân cấp được định vị về phía góc dưới bên phải và diện tích dưới đường cong là 0,19, cả hai đều cho thấy mức độ chính xác và phân biệt tổng thể thấp. So với PLR, tỷ lệ khả năng nghịch đảo âm (1/LR-) chỉ ra rằng một kết quả dương tính có tác động lớn hơn đến tỷ lệ mắc bệnh so với kết quả âm tính. Kết luận Tăng bilirubin máu đơn thuần không phải là một công cụ đáng tin cậy để dự đoán thủng. Các nghiên cứu trong tương lai nên điều tra xem các giá trị dự đoán kết hợp của bilirubin, protein phản ứng C (CRP) và bạch cầu có phải là một công cụ chẩn đoán hiệu quả hơn hay không."} +{"idx": 1910122, "en": "Background We investigated the relationship between the preoperative psychological state and the perioperative nutritional conditions of patients with esophageal cancer. Methods Seventy-three participants underwent operations for esophageal cancer in our hospital. Depressive state was evaluated using the Self-Rating Depression Scale (SDS). General quality of life (QOL) was assessed using the SF-8™, and the nutritional assessments were evaluated through anthropometric analysis, bioelectrical impedance analysis (BIA) and some biochemical assessments. Results In the preoperative stage, patients with higher SDS scores, representing a more depressive state, had low arm circumference, grip strength, serum albumin levels and prognostic nutritional index. Patients with higher SDS scores also had a tendency for a lower physical component summary, representing physical QOL by the Eight-Item Short Form Health Survey (SF-8™). At 3 months after surgery, patients with higher preoperative SDS scores had significantly lower body mass indexes (BMIs) and had a lower tendency of body fat masses. In the univariate and multivariate analyses on the recovery of BMI at 3 months after surgery, preoperative SDS score was the only independent risk factor (odd ratio (OR): 4.07, 95% confidence interval (CI): 1.15 - 14.35) in this study. Conclusion Preoperative depressive mood, as evaluated by the SDS, was the sole relevant factor for postoperative body weight recovery of patients with esophageal cancer. Preoperative depressive mood of patients with esophageal cancer might delay recovery from operation-related malnutrition. Some measures against preoperative depressive mood might be necessary for early recovery from postoperative malnutrition in patients with esophageal cancer.", "gemini": "Bối cảnh Chúng tôi đã nghiên cứu mối quan hệ giữa trạng thái tâm lý trước phẫu thuật và tình trạng dinh dưỡng quanh phẫu thuật của bệnh nhân ung thư thực quản. Phương pháp Bảy mươi ba người tham gia đã được phẫu thuật ung thư thực quản tại bệnh viện của chúng tôi. Trạng thái trầm cảm được đánh giá bằng Thang đánh giá trầm cảm tự đánh giá (SDS). Chất lượng cuộc sống chung (QOL) được đánh giá bằng SF-8™, và đánh giá dinh dưỡng được đánh giá thông qua phân tích nhân trắc học, phân tích trở kháng điện sinh học (BIA) và một số đánh giá sinh hóa. Kết quả Ở giai đoạn trước phẫu thuật, những bệnh nhân có điểm SDS cao hơn, biểu thị trạng thái trầm cảm nhiều hơn, có chu vi cánh tay, sức nắm, nồng độ albumin huyết thanh và chỉ số dinh dưỡng tiên lượng thấp. Những bệnh nhân có điểm SDS cao hơn cũng có xu hướng có tóm tắt thành phần vật lý thấp hơn, biểu thị QOL về thể chất bằng Khảo sát sức khỏe dạng ngắn tám mục (SF-8™). Vào thời điểm 3 tháng sau phẫu thuật, những bệnh nhân có điểm SDS trước phẫu thuật cao hơn có chỉ số khối cơ thể (BMI) thấp hơn đáng kể và có xu hướng khối lượng mỡ cơ thể thấp hơn. Trong các phân tích đơn biến và đa biến về sự phục hồi BMI ở thời điểm 3 tháng sau phẫu thuật, điểm SDS trước phẫu thuật là yếu tố nguy cơ độc lập duy nhất (tỷ lệ nguy cơ (OR): 4,07, khoảng tin cậy 95% (CI): 1,15 - 14,35) trong nghiên cứu này. Kết luận Tâm trạng trầm cảm trước phẫu thuật, được đánh giá bằng SDS, là yếu tố có liên quan duy nhất đối với sự phục hồi cân nặng sau phẫu thuật của bệnh nhân ung thư thực quản. Tâm trạng trầm cảm trước phẫu thuật của bệnh nhân ung thư thực quản có thể làm chậm quá trình phục hồi sau tình trạng suy dinh dưỡng liên quan đến phẫu thuật. Một số biện pháp chống lại tâm trạng trầm cảm trước phẫu thuật có thể cần thiết để phục hồi sớm sau tình trạng suy dinh dưỡng sau phẫu thuật ở bệnh nhân ung thư thực quản."} +{"idx": 1910123, "en": "Background To determine the association of infection with human papillomavirus (HPV) and the occupation of female sex worker; and the correlation of infection with HPV with sociodemographic, clinical and behavioral characteristics of female sex workers. Methods We performed a case-control study of 217 female sex workers and 354 women without sex work in Durango City, Mexico. We determined the prevalence of infection with HPV in cervical samples of women using polymerase chain reaction, and HPV genotypes were determined using line probe assay. Bivariate and multivariate analyses were used to assess the association between the characteristics of women and infection. Results Twelve (5.5%) of the 217 sex workers, and 10 (2.8%) of the 354 control women were positive for HPV DNA (age-adjusted OR = 1.51; 95% CI: 0.62 - 3.68; P = 0.36). Six (50.0%) of the 12 HPV DNA positive sex workers had infections with high-risk genotypes (16, 31, 33, 35, 51, 58). Seven (70%) of the 10 HPV DNA positive control women had infections with high-risk genotypes (16, 18, 56, 58, and 66). The frequency of high risk genotypes in the control women was equal with that found in the female sex workers (P = 0.41). Logistic regression analysis showed that the variable alcohol consumption was associated with HPV infection (OR = 4.0; 95% CI: 1.0 - 16.0; P = 0.04). Conclusions No association between HPV infection and female sex work was found in our setting. High risk HPV genotypes were prevalent among the women studied. Results can be used for the design of preventive measures against HPV infection.", "gemini": "Mục tiêu Để xác định mối liên quan giữa nhiễm trùng với vi-rút papilloma ở người (HPV) và nghề nghiệp của nữ hành nghề mại dâm; và mối tương quan giữa nhiễm trùng với HPV với các đặc điểm xã hội nhân khẩu học, lâm sàng và hành vi của nữ hành nghề mại dâm. Phương pháp Chúng tôi đã thực hiện một nghiên cứu đối chứng có nhóm chứng gồm 217 nữ hành nghề mại dâm và 354 phụ nữ không hành nghề mại dâm tại Thành phố Durango, Mexico. Chúng tôi xác định tỷ lệ nhiễm trùng HPV trong các mẫu cổ tử cung của phụ nữ bằng cách sử dụng phản ứng chuỗi polymerase và các kiểu gen HPV được xác định bằng xét nghiệm thăm dò dòng. Phân tích song biến và đa biến được sử dụng để đánh giá mối liên quan giữa các đặc điểm của phụ nữ và nhiễm trùng. Kết quả Mười hai (5,5%) trong số 217 nữ hành nghề mại dâm và 10 (2,8%) trong số 354 phụ nữ đối chứng có kết quả xét nghiệm dương tính với DNA HPV (OR hiệu chỉnh theo tuổi = 1,51; KTC 95%: 0,62 - 3,68; P = 0,36). Sáu (50,0%) trong số 12 nữ hành nghề mại dâm có DNA HPV dương tính bị nhiễm trùng với các kiểu gen có nguy cơ cao (16, 31, 33, 35, 51, 58). Bảy (70%) trong số 10 phụ nữ đối chứng có DNA HPV dương tính bị nhiễm trùng với các kiểu gen có nguy cơ cao (16, 18, 56, 58 và 66). Tần suất các kiểu gen có nguy cơ cao ở những phụ nữ đối chứng bằng với tần suất tìm thấy ở những nữ hành nghề mại dâm (P = 0,41). Phân tích hồi quy logistic cho thấy biến tiêu thụ rượu có liên quan đến nhiễm HPV (OR = 4,0; KTC 95%: 1,0 - 16,0; P = 0,04). Kết luận Không tìm thấy mối liên quan giữa nhiễm HPV và hành nghề mại dâm của nữ trong nghiên cứu của chúng tôi. Các kiểu gen HPV có nguy cơ cao rất phổ biến ở những phụ nữ được nghiên cứu. Kết quả có thể được sử dụng để thiết kế các biện pháp phòng ngừa chống lại nhiễm HPV."} +{"idx": 1910124, "en": "Background Despite the availability of antihypertensive treatments, fewer than half of patients who receive treatment successfully achieve blood pressure (BP) goals. The purpose of this study was to evaluate the effect of switching to azilsartan 40 mg from a fixed-dose combination tablet of an angiotensin II receptor blocker (ARB) and amlodipine at 5 mg or azelnidipine at 16 mg (ARB/CCB) or an ARB and hydrochlorothiazide (HCT) at 6.25 mg or 12.5 mg (ARB/HCT) on BP. Methods This prospective, multicenter, open-labeled, single-arm study included 40 patients treated with a fixed-dose combination tablet of an ARB/CCB or an ARB/HCT, which was switched to azilsartan 40 mg after enrollment. The primary outcome was the change in BP from baseline to the 24-week follow-up. Results Of the 40 patients who completed this study, 33 did not require changes in their antihypertensive medications after switching to azilsartan, and their BP did not change from baseline to follow-up. However, the systolic BP in seven patients was elevated at 12 weeks, and amlodipine at 5 mg was therefore added; these patients' baseline medications were an ARB/CCB (n = 6) and an ARB/HCT (n = 1). In all patients, the serum potassium level was slightly increased after switching to azilsartan at 6 months, while the serum creatinine, hemoblobinA1c, and lipid profile did not change. Conclusions Azilsartan at 40 mg did not result in a greater decrease in BP than a fixed-dose combination tablet of an ARB/CCB or an ARB/HCT. However, our findings suggest a substantial BP-lowering effect of azilsartan at 40 mg in patients with hypertension.", "gemini": "Mục tiêu Mặc dù có sẵn các phương pháp điều trị tăng huyết áp, nhưng chưa đến một nửa số bệnh nhân được điều trị đạt được mục tiêu huyết áp (HA). Mục đích của nghiên cứu này là đánh giá hiệu quả của việc chuyển sang azilsartan 40 mg từ viên thuốc kết hợp liều cố định của thuốc chẹn thụ thể angiotensin II (ARB) và amlodipine ở liều 5 mg hoặc azelnidipine ở liều 16 mg (ARB/CCB) hoặc ARB và hydrochlorothiazide (HCT) ở liều 6,25 mg hoặc 12,5 mg (ARB/HCT) đối với HA. Phương pháp Nghiên cứu triển vọng, đa trung tâm, nhãn mở, một nhóm này bao gồm 40 bệnh nhân được điều trị bằng viên thuốc kết hợp liều cố định ARB/CCB hoặc ARB/HCT, được chuyển sang azilsartan 40 mg sau khi đăng ký. Kết quả chính là sự thay đổi HA từ ban đầu đến thời điểm theo dõi sau 24 tuần. Kết quả Trong số 40 bệnh nhân hoàn thành nghiên cứu này, 33 bệnh nhân không cần thay đổi thuốc chống tăng huyết áp sau khi chuyển sang azilsartan và HA của họ không thay đổi từ ban đầu đến khi theo dõi. Tuy nhiên, HA tâm thu ở bảy bệnh nhân tăng lên ở tuần thứ 12 và do đó, amlodipine ở liều 5 mg đã được thêm vào; các loại thuốc ban đầu của những bệnh nhân này là ARB/CCB (n = 6) và ARB/HCT (n = 1). Ở tất cả bệnh nhân, nồng độ kali trong huyết thanh tăng nhẹ sau khi chuyển sang azilsartan ở tháng thứ 6, trong khi creatinin huyết thanh, hemoblobinA1c và hồ sơ lipid không thay đổi. Kết luận Azilsartan ở liều 40 mg không làm giảm HA nhiều hơn viên thuốc kết hợp liều cố định ARB/CCB hoặc ARB/HCT. Tuy nhiên, những phát hiện của chúng tôi cho thấy hiệu quả hạ HA đáng kể của azilsartan ở liều 40 mg ở những bệnh nhân tăng huyết áp."} +{"idx": 1910125, "en": "Background Nephrolithiasis or kidney stone disease (KSD) is a common disorder worldwide. Despite the availability of a variety of effective management strategies, KSD recurrence remains a problem. In the present study, we investigated the KSD recurrence after the treatment of the first stone episode. Methods Medical records of all patients who had KDS treated in our department from January 2012 to January 2016 were retrospectively reviewed. A total of 146 patients who had KDS recurrence were identified. The demographic information, biochemical data, treatment methods, report of hydronephrosis severity and stone chemical compositions were collected and analyzed. Results We reported that: 1) Sixty four (43.8%) out of 146 patients with stone recurrence were overweight or obese; 2) Of all 146 patients with stone recurrence, 86 (58.9%) had hyperlipidemia, 77 (52.4%) had hyperuricemia and 64 (43.8%) had hyperglycemia; 3) Mini-invasive methods were mostly used for the treatment of the first stone episode; 4) The most chief complaint differed during the first episode and recurrence; 5) The number of patients who had grades 3 and 4 hydronephrosis was significantly lower during recurrence compared with that in the first stone episode; 6) Seventy nine (54.1%) patients with recurrence had stone of calcium oxalate. Conclusions Majority of patients with KSD recurrence have overweight/obesity, hyperlipidemia, hyperuricemia and hyperglycemia, indicating a role of metabolic disorders in stone recurrence. Mini-invasive methods are the mainstay for the management of the first stone episode. The degree of hydronephrosis is significantly reduced during stone recurrence, possibly due to increased awareness of the disease and thereafter the change of lifestyle in patients. Over half of recurrent stones are of calcium oxalate.", "gemini": "Mục tiêu Sỏi thận hoặc bệnh sỏi thận (KSD) là một rối loạn phổ biến trên toàn thế giới. Mặc dù có nhiều chiến lược quản lý hiệu quả, nhưng tái phát KSD vẫn là một vấn đề. Trong nghiên cứu hiện tại, chúng tôi đã điều tra tình trạng tái phát KSD sau khi điều trị đợt sỏi đầu tiên. Phương pháp Hồ sơ bệnh án của tất cả bệnh nhân được điều trị KDS tại khoa của chúng tôi từ tháng 1 năm 2012 đến tháng 1 năm 2016 đã được xem xét lại. Tổng cộng 146 bệnh nhân tái phát KDS đã được xác định. Thông tin nhân khẩu học, dữ liệu sinh hóa, phương pháp điều trị, báo cáo mức độ giãn đài thận và thành phần hóa học của sỏi đã được thu thập và phân tích. Kết quả Chúng tôi báo cáo rằng: 1) Sáu mươi bốn (43,8%) trong số 146 bệnh nhân tái phát sỏi thừa cân hoặc béo phì; 2) Trong số tất cả 146 bệnh nhân tái phát sỏi, 86 (58,9%) bị tăng lipid máu, 77 (52,4%) bị tăng acid uric máu và 64 (43,8%) bị tăng đường huyết; 3) Các phương pháp xâm lấn tối thiểu chủ yếu được sử dụng để điều trị đợt sỏi đầu tiên; 4) Triệu chứng chính khác nhau trong đợt đầu tiên và tái phát; 5) Số bệnh nhân bị giãn đài thận độ 3 và 4 giảm đáng kể trong khi tái phát so với đợt sỏi đầu tiên; 6) Bảy mươi chín (54,1%) bệnh nhân tái phát có sỏi canxi oxalat. Kết luận Phần lớn bệnh nhân tái phát KSD bị thừa cân/béo phì, tăng lipid máu, tăng acid uric máu và tăng đường huyết, cho thấy vai trò của rối loạn chuyển hóa trong tình trạng tái phát sỏi. Các phương pháp xâm lấn tối thiểu là phương pháp chính để quản lý đợt sỏi đầu tiên. Mức độ giãn đài thận giảm đáng kể trong khi tái phát sỏi, có thể là do tăng nhận thức về bệnh và sau đó thay đổi lối sống ở bệnh nhân. Hơn một nửa số sỏi tái phát là canxi oxalat."} +{"idx": 1910126, "en": "Background The aim of the study was to investigate risk factors of hypoglycemic encephalopathy (HE) in patients with severe hypoglycemia. Methods We retrospectively enrolled patients with severe hypoglycemia who were transported to the emergency department in an ambulance. We defined severe hypoglycemia as plasma glucose level < 60 mg/dL (or capillary levels < 50 mg/dL). HE was defined as severe hypoglycemia with altered level of consciousness (Glasgow coma scale < 12) and prolonged HE as coma or stupor lasting > 24 h after glucose administration. We compared several parameters between patients with and without HE and between prolonged and recovered patients. Results Included were 173 patients with severe hypoglycemia; of them, 94 were diagnosed with HE, with 12 of them prolonged HE. Glucose level in HE patients was lower than that in those without HE (P < 0.001). Moreover, we noted a significant difference in glucose levels between the prolonged and recovered groups. Furthermore, body temperature was higher in prolonged versus recovered patients (P = 0.0017). Conclusion Blood glucose level may be correlated with severity of altered level of consciousness. In addition, body temperature may be related to coma or prolonged stupor.", "gemini": "Bối cảnh Mục tiêu của nghiên cứu này là điều tra các yếu tố nguy cơ của bệnh não do hạ đường huyết (HE) ở những bệnh nhân bị hạ đường huyết nặng. Phương pháp Chúng tôi đã tuyển dụng những bệnh nhân bị hạ đường huyết nặng được đưa đến khoa cấp cứu bằng xe cứu thương. Chúng tôi định nghĩa hạ đường huyết nặng là mức glucose huyết tương < 60 mg/dL (hoặc mức mao mạch < 50 mg/dL). HE được định nghĩa là hạ đường huyết nặng với tình trạng thay đổi mức độ ý thức (điểm hôn mê Glasgow < 12) và HE kéo dài là hôn mê hoặc lú kéo dài > 24 giờ sau khi dùng glucose. Chúng tôi đã so sánh một số thông số giữa những bệnh nhân có và không có HE và giữa những bệnh nhân kéo dài và hồi phục. Kết quả Có 173 bệnh nhân bị hạ đường huyết nặng được đưa vào; trong số đó, 94 bệnh nhân được chẩn đoán mắc HE, với 12 trường hợp HE kéo dài. Nồng độ glucose ở bệnh nhân HE thấp hơn so với những bệnh nhân không bị HE (P < 0,001). Hơn nữa, chúng tôi đã ghi nhận sự khác biệt đáng kể về nồng độ glucose giữa nhóm kéo dài và nhóm hồi phục. Hơn nữa, nhiệt độ cơ thể cao hơn ở những bệnh nhân kéo dài so với những bệnh nhân hồi phục (P = 0,0017). Kết luận Nồng độ glucose trong máu có thể tương quan với mức độ nghiêm trọng của tình trạng thay đổi ý thức. Ngoài ra, nhiệt độ cơ thể có thể liên quan đến hôn mê hoặc lú kéo dài."} +{"idx": 1910127, "en": "Haploidentical hematopoietic stem cell transplantation (haplo-HSCT) with post-transplant cyclophosphamide (PTCy) offers universal donor availability and can potentially cure relapsed or primary refractory Hodgkin lymphoma (HL) and non-Hodgkin lymphoma (NHL). However, a conditioning regimen intensity that balances the graft-versus-lymphoma (GvL) effect with regimen-related toxicities (RRTs) has not yet been optimized. Limited data exist on the management of relapse, which is common post-transplant. Few prospective or randomized control trials have been conducted on lymphoma patients undergoing haplo-HSCT. Therefore, the current review aims to summarize published retrospective data in the field to help guide clinical decision making for high-risk patients. Retrospective studies in the field are characterized by variability in patient population and sample sizes, eligibility criteria, number of prior treatments (e.g., chemotherapy, radiation therapy, and autologous transplant), graft source (bone marrow or peripheral blood), as well as choice and intensity of the conditioning regimen (non-myeloablative, reduced intensity, or myeloablative). Nonetheless, common themes that emerge from the literature include: 1) Enhanced donor availability and selection with haplo-HSCT with success in heterogeneous patient populations; 2) Outcomes that are comparable if not superior to matched related (MRD) or unrelated (MUD) donor transplants; 3) The benefit of PTCy for reducing incidence of relapse and chronic graft-versus-host disease (GvHD); 4) Presence of co-morbidities leading to poorer transplant-related outcomes; and 5) The need for novel approaches to address disease relapse, particularly for patients with active disease at the time of transplant. Excellent transplant-related outcomes with haplo-HSCT with PTCy have been seen for HL and NHL based on retrospective data. Further studies are needed to determine integration with advanced cellular therapy techniques, such as chimeric antigen receptor (CAR) T-cell, antibody drug conjugates, and checkpoint inhibitors. Graft manipulation may be another avenue for future research.", "gemini": "Ghép tế bào gốc tạo máu nửa hợp tử (haplo-HSCT) với cyclophosphamide sau ghép (PTCy) cung cấp khả năng tiếp cận của người hiến tặng phổ quát và có khả năng chữa khỏi bệnh u lympho Hodgkin (HL) tái phát hoặc kháng thuốc nguyên phát và u lympho không Hodgkin (NHL). Tuy nhiên, cường độ chế độ gây mê cân bằng hiệu ứng ghép chống lại u lympho (GvL) với độc tính liên quan đến chế độ (RRT) vẫn chưa được tối ưu hóa. Dữ liệu hạn chế về việc quản lý tái phát, thường gặp sau ghép. Một số ít thử nghiệm đối chứng ngẫu nhiên hoặc có triển vọng đã được tiến hành trên bệnh nhân u lympho đang trải qua haplo-HSCT. Do đó, mục đích của bài đánh giá hiện tại là tóm tắt dữ liệu hồi cứu đã công bố trong lĩnh vực này để giúp hướng dẫn việc ra quyết định lâm sàng cho những bệnh nhân có nguy cơ cao. Các nghiên cứu hồi cứu trong lĩnh vực này được đặc trưng bởi sự thay đổi về quần thể bệnh nhân và quy mô mẫu, tiêu chuẩn đủ điều kiện, số lượng phương pháp điều trị trước đó (ví dụ: hóa trị, xạ trị và ghép tự thân), nguồn ghép (tủy xương hoặc máu ngoại vi), cũng như sự lựa chọn và cường độ của chế độ gây mê (không gây mất tủy, cường độ giảm hoặc gây mất tủy). Tuy nhiên, các chủ đề chung nổi lên từ các tài liệu bao gồm: 1) Khả năng tiếp cận và lựa chọn người hiến tặng được tăng cường với haplo-HSCT với thành công ở các quần thể bệnh nhân không đồng nhất; 2) Kết quả có thể so sánh nếu không muốn nói là vượt trội so với ghép từ người hiến tặng có quan hệ (MRD) hoặc không có quan hệ (MUD); 3) Lợi ích của PTCy trong việc giảm tỷ lệ tái phát và bệnh ghép chống vật chủ mạn tính (GvHD); 4) Sự hiện diện của các bệnh đi kèm dẫn đến kết quả ghép tệ hơn; và 5) Sự cần thiết của các phương pháp tiếp cận mới để giải quyết tình trạng tái phát bệnh, đặc biệt là đối với những bệnh nhân mắc bệnh tích cực tại thời điểm ghép. Kết quả ghép tuyệt vời với haplo-HSCT với PTCy đã được thấy đối với HL và NHL dựa trên dữ liệu hồi cứu. Cần nghiên cứu thêm để xác định sự tích hợp với các kỹ thuật điều trị tế bào tiên tiến, chẳng hạn như tế bào T thụ thể kháng nguyên chimeric (CAR), liên hợp thuốc kháng thể và chất ức chế điểm kiểm tra. Thao tác ghép có thể là một hướng nghiên cứu khác trong tương lai."} +{"idx": 1910128, "en": "Although a rare and challenging condition, cancer during pregnancy should promptly be identified and treated. Not only standards of care guidelines for the underlying disease are taken into account, but also fetal safety might be weighted for clinical decisions. Frequent lack of experience and knowledge about this condition could lead to late diagnosis, imprecise management, suboptimal treatment and fetal and maternal harm. Therefore, this review aims to summarize the current evidence regarding the epidemiology, clinical presentation, diagnostic workup, staging and treatment, including novel treatment modalities for patients diagnosed with cancer during pregnancy.", "gemini": "Mặc dù là một tình trạng hiếm gặp và đầy thách thức, ung thư trong thai kỳ cần được xác định và điều trị kịp thời. Không chỉ các hướng dẫn về tiêu chuẩn chăm sóc cho bệnh nền được tính đến, mà sự an toàn của thai nhi cũng có thể được cân nhắc cho các quyết định lâm sàng. Việc thường xuyên thiếu kinh nghiệm và kiến ​​thức về tình trạng này có thể dẫn đến chẩn đoán muộn, quản lý không chính xác, điều trị không tối ưu và gây hại cho thai nhi và bà mẹ. Do đó, mục đích của bài đánh giá này là tóm tắt các bằng chứng hiện tại về dịch tễ học, biểu hiện lâm sàng, quá trình chẩn đoán, phân giai và điều trị, bao gồm các phương thức điều trị mới cho những bệnh nhân được chẩn đoán mắc bệnh ung thư trong thai kỳ."} +{"idx": 1910129, "en": "Pancreatic cancer is the seventh leading cause of cancer-related deaths worldwide. However, its toll is higher in more developed countries. Reasons for vast differences in mortality rates of pancreatic cancer are not completely clear yet, but it may be due to lack of appropriate diagnosis, treatment and cataloging of cancer cases. Because patients seldom exhibit symptoms until an advanced stage of the disease, pancreatic cancer remains one of the most lethal malignant neoplasms that caused 432,242 new deaths in 2018 (GLOBOCAN 2018 estimates). Globally, 458,918 new cases of pancreatic cancer have been reported in 2018, and 355,317 new cases are estimated to occur until 2040. Despite advancements in the detection and management of pancreatic cancer, the 5-year survival rate still stands at 9% only. To date, the causes of pancreatic carcinoma are still insufficiently known, although certain risk factors have been identified, such as tobacco smoking, diabetes mellitus, obesity, dietary factors, alcohol abuse, age, ethnicity, family history and genetic factors, Helicobacter pylori infection, non-O blood group and chronic pancreatitis. In general population, screening of large groups is not considered useful to detect the disease at its early stage, although newer techniques and the screening of tightly targeted groups (especially of those with family history), are being evaluated. Primary prevention is considered of utmost importance. Up-to-date statistics on pancreatic cancer occurrence and outcome along with a better understanding of the etiology and identifying the causative risk factors are essential for the primary prevention of this disease.", "gemini": "Ung thư tụy là nguyên nhân gây tử vong do ung thư đứng thứ bảy trên toàn thế giới. Tuy nhiên, số ca tử vong do ung thư tụy cao hơn ở các nước phát triển. Nguyên nhân dẫn đến sự chênh lệch lớn về tỷ lệ tử vong do ung thư tụy vẫn chưa được làm rõ hoàn toàn, nhưng có thể là do thiếu chẩn đoán, điều trị và lập danh mục các trường hợp ung thư phù hợp. Vì bệnh nhân hiếm khi biểu hiện triệu chứng cho đến giai đoạn tiến triển của bệnh, nên ung thư tụy vẫn là một trong những khối u ác tính gây tử vong nhiều nhất, gây ra 432.242 ca tử vong mới vào năm 2018 (ước tính của GLOBOCAN 2018). Trên toàn cầu, đã có 458.918 ca ung thư tụy mới được báo cáo vào năm 2018 và ước tính có 355.317 ca mới xảy ra cho đến năm 2040. Mặc dù có những tiến bộ trong việc phát hiện và điều trị ung thư tụy, tỷ lệ sống sót sau 5 năm vẫn chỉ ở mức 9%. Cho đến nay, nguyên nhân gây ung thư biểu mô tụy vẫn chưa được biết đầy đủ, mặc dù một số yếu tố nguy cơ đã được xác định, chẳng hạn như hút thuốc lá, đái tháo đường, béo phì, các yếu tố chế độ ăn uống, lạm dụng rượu, tuổi tác, dân tộc, tiền sử gia đình và yếu tố di truyền, nhiễm Helicobacter pylori, nhóm máu không phải nhóm O và viêm tụy mãn tính. Trong dân số nói chung, việc sàng lọc các nhóm lớn không được coi là hữu ích để phát hiện bệnh ở giai đoạn đầu, mặc dù các kỹ thuật mới hơn và việc sàng lọc các nhóm có mục tiêu chặt chẽ (đặc biệt là những người có tiền sử gia đình) đang được đánh giá. Phòng ngừa tiên phát được coi là tối quan trọng. Số liệu thống kê cập nhật về tỷ lệ mắc và kết cục của ung thư tụy cùng với việc hiểu rõ hơn về nguyên nhân và xác định các yếu tố nguy cơ gây bệnh là rất cần thiết để phòng ngừa tiên phát căn bệnh này."} +{"idx": 1910130, "en": "Background Information regarding testosterone as a significant risk factor of cardiovascular disease (CVD) in female patients with type 2 diabetes mellitus (DM) is limited. However, some clinical studies reported the importance of cardio-ankle vascular index (CAVI) as a novel physiological marker of arterial function in type 2 DM. This cross-sectional study aimed to elucidate the clinical effects of blood testosterone concentration on CAVI in female patients with type 2 DM. Methods A total of 238 postmenopausal patients including 97 with a history of CVD with type 2 DM (age (mean ± standard deviation (SD)), 73 ± 9 years) were enrolled. CAVI was measured according to the standard technique, and serum total testosterone concentration (T-T) was also measured as a testosterone level marker in vivo. The relationship between CAVI and T-T was evaluated. Results CAVI is significantly higher (CVD vs. non-CVD: 10.2 ± 1.2 vs. 9.2 ± 1.0, P < 0.001), and log-T-T significantly lower (CVD vs. non-CVD: 1.2 ± 0.2 ng/dL vs. 1.5 ± 0.2 ng/dL, P < 0.001) in patients with CVD than those without CVD. CAVI was significantly negatively correlated with log-T-T (r = -0.41; P < 0.001). Furthermore, multiple regression analysis indicated that CVD (β = 0.23; P < 0.001) and log-T-T (β = -0.18; P < 0.01) were selected as independent subordinate variables for CAVI. Conclusions This study showed that T-T was independently inversely associated with CAVI, indicating that low testosterone concentration is a considerable risk factor for the progression of arterial dysfunction in female patients with type 2 DM.", "gemini": "Thông tin cơ bản về testosterone như một yếu tố nguy cơ đáng kể của bệnh tim mạch (CVD) ở bệnh nhân nữ mắc bệnh đái tháo đường týp 2 (ĐTĐ) còn hạn chế. Tuy nhiên, một số nghiên cứu lâm sàng đã báo cáo tầm quan trọng của chỉ số mạch máu mắt cá chân tim (CAVI) như một dấu ấn sinh lý mới về chức năng động mạch ở bệnh nhân ĐTĐ týp 2. Nghiên cứu cắt ngang này nhằm làm sáng tỏ các tác động lâm sàng của nồng độ testosterone trong máu đối với CAVI ở bệnh nhân nữ mắc ĐTĐ týp 2. Phương pháp Tổng cộng có 238 bệnh nhân sau mãn kinh, trong đó có 97 bệnh nhân có tiền sử mắc CVD kèm ĐTĐ týp 2 (tuổi (trung bình ± độ lệch chuẩn (SD)), 73 ± 9 tuổi) đã được tuyển dụng. CAVI được đo theo kỹ thuật chuẩn và nồng độ testosterone toàn phần trong huyết thanh (T-T) cũng được đo như một dấu ấn về mức testosterone trong cơ thể sống. Mối quan hệ giữa CAVI và T-T đã được đánh giá. Kết quả CAVI cao hơn đáng kể (CVD so với không CVD: 10,2 ± 1,2 so với 9,2 ± 1,0, P < 0,001) và log-T-T thấp hơn đáng kể (CVD so với không CVD: 1,2 ± 0,2 ng/dL so với 1,5 ± 0,2 ng/dL, P < 0,001) ở bệnh nhân mắc CVD so với những bệnh nhân không mắc CVD. CAVI có tương quan âm đáng kể với log-T-T (r = -0,41; P < 0,001). Hơn nữa, phân tích hồi quy đa biến chỉ ra rằng CVD (β = 0,23; P < 0,001) và log-T-T (β = -0,18; P < 0,01) được chọn làm các biến phụ thuộc độc lập cho CAVI. Kết luận Nghiên cứu này cho thấy T-T có liên quan nghịch đảo độc lập với CAVI, chỉ ra rằng nồng độ testosterone thấp là một yếu tố nguy cơ đáng kể đối với tiến triển của rối loạn chức năng động mạch ở bệnh nhân nữ mắc ĐTĐ týp 2."} +{"idx": 1910131, "en": "Background We aimed to compare outcomes of patients received successful fibrinolytic treatment (FT) for ST-segment elevated myocardial infarction (STEMI) and performed coronary angiography (CAG) within 24 - 72 h or after 72 h. Methods Between March 2013 and November 2014, 76 STEMI patients received successful FT and performed CAG > 24 h were included in the study. Patients were divided into two groups according to the time-interval from FT admission to CAG performing (Group-1, 24 - 72 h (n = 29), Group-2, > 72 h (n = 47)). The primary end point was major adverse cardiac events (MACE) defined as cardiovascular death, non-fatal myocardial infarction, and heart failure. Results The mean age of patients were 56 ± 11.4 years old (27.6% female). CAG was performed within mean 2.17 ± 0.38 days in the Group-1 and 2.9 ± 11.5 days in the Group 2 (P < 0.001). At short-term follow-up (6 months), MACE rate was higher in Group-2 (21.3%) than Group-1(13.8%), but it was not statistically significant (P = 0.661). The rate of MACE was 37.9% in Group-1 and 38.3% in Group-2 (P = 0.974) in the long-term follow-up (median: 57 months). Overall cardiac mortality rate was 7.9%, the re-infarction rate was 19.7% and heart failure was 17.1% in long-term follow-up, and there were no significant difference between groups. Conclusions Present study has shown that performance of CAG after 24 h of successful FT, within 24 - 74 h or > 72 h, did not shown any difference in term of MACE both in short and long-term follow-up.", "gemini": "Bối cảnh Chúng tôi nhằm mục đích so sánh kết cục của bệnh nhân được điều trị thành công bằng thuốc tiêu sợi huyết (FT) đối với nhồi máu cơ tim có ST chênh lệch (STEMI) và chụp động mạch vành (CAG) trong vòng 24 - 72 giờ hoặc sau 72 giờ. Phương pháp Từ tháng 3 năm 2013 đến tháng 11 năm 2014, 76 bệnh nhân STEMI được điều trị thành công bằng FT và chụp CAG > 24 giờ đã được đưa vào nghiên cứu. Bệnh nhân được chia thành hai nhóm theo khoảng thời gian từ khi nhập viện FT đến khi thực hiện CAG (Nhóm 1, 24 - 72 giờ (n = 29), Nhóm 2, > 72 giờ (n = 47)). Điểm cuối chính là các biến cố tim mạch lớn (MACE) được định nghĩa là tử vong do tim mạch, nhồi máu cơ tim không tử vong và suy tim. Kết quả Tuổi trung bình của bệnh nhân là 56 ± 11,4 tuổi (27,6% là nữ). CAG được thực hiện trong vòng trung bình 2,17 ± 0,38 ngày ở Nhóm 1 và 2,9 ± 11,5 ngày ở Nhóm 2 (P < 0,001). Trong thời gian theo dõi ngắn hạn (6 tháng), tỷ lệ MACE cao hơn ở Nhóm 2 (21,3%) so với Nhóm 1 (13,8%), nhưng không có ý nghĩa thống kê (P = 0,661). Tỷ lệ MACE là 37,9% ở Nhóm 1 và 38,3% ở Nhóm 2 (P = 0,974) trong thời gian theo dõi dài hạn (trung bình: 57 tháng). Tỷ lệ tử vong do tim nói chung là 7,9%, tỷ lệ tái nhồi máu là 19,7% và suy tim là 17,1% trong thời gian theo dõi dài hạn, và không có sự khác biệt đáng kể giữa các nhóm. Kết luận Nghiên cứu hiện tại đã chỉ ra rằng việc thực hiện CAG sau 24 giờ điều trị FT thành công, trong vòng 24 - 74 giờ hoặc > 72 giờ, không cho thấy bất kỳ sự khác biệt nào về MACE trong cả thời gian theo dõi ngắn hạn và dài hạn."} +{"idx": 1910132, "en": "Background Although β-blocker treatment is generally contraindicated in patients presenting with acute cocaine intoxication due to concern for unopposed α-receptor stimulation, some studies have reported that β-blocker treatment did not increase adverse events in these patients. As this treatment is still controversial, we performed a meta-analysis of observational studies on this topic. Methods By searching three electronic databases (MEDLINE, EMBASE, and the Cochrane Library) from their inception to June 11, 2018, we identified eight observational studies with 2,048 patients who presented to hospital with cocaine-associated chest pain or after recent cocaine use. Outcomes of interest were myocardial necrosis or infarction (MI) and death during hospital stay or follow-up. Pooled relative risks (RRs) with 95% confidence intervals (CIs) were calculated by using a random-effects meta-analysis based on the DerSimonian-Laird method. Results Among patients presenting with cocaine-associated chest pain or recent cocaine use, there was no significant difference in in-hospital all-cause mortality (RR, 0.59; 95% CI, 0.24 - 1.47) and MI (RR, 1.24; 95% CI, 0.74 - 2.06) between patients who did and did not receive β-blocker treatment during their hospital stay. During long-term follow-up (mean 2.6 years), there was no significant difference in all-cause mortality (RR, 0.79; 95% CI, 0.44 - 1.41) and MI (RR, 0.96; 95% CI, 0.40 - 2.33) between the two groups. Conclusions These results suggest that β-blocker treatment in patients presenting with cocaine intoxication may not be as harmful as originally believed. Further clinical studies are needed to investigate this topic.", "gemini": "Mặc dù việc điều trị bằng thuốc chẹn β thường chống chỉ định ở những bệnh nhân bị ngộ độc cocaine cấp do lo ngại về sự kích thích thụ thể α không bị cản trở, một số nghiên cứu đã báo cáo rằng việc điều trị bằng thuốc chẹn β không làm tăng các tác dụng phụ ở những bệnh nhân này. Vì phương pháp điều trị này vẫn còn gây tranh cãi, chúng tôi đã thực hiện một phân tích tổng hợp các nghiên cứu quan sát về chủ đề này. Phương pháp Bằng cách tìm kiếm ba cơ sở dữ liệu điện tử (MEDLINE, EMBASE và Thư viện Cochrane) từ khi bắt đầu đến ngày 11 tháng 6 năm 2018, chúng tôi đã xác định được tám nghiên cứu quan sát với 2.048 bệnh nhân nhập viện vì đau ngực do cocaine hoặc sau khi sử dụng cocaine gần đây. Kết quả quan tâm là nhồi máu cơ tim hoặc nhồi máu cơ tim (MI) và tử vong trong thời gian nằm viện hoặc theo dõi. Các tỷ lệ nguy cơ tương đối gộp (RR) với khoảng tin cậy 95% (CI) được tính bằng cách sử dụng phân tích tổng hợp hiệu ứng ngẫu nhiên dựa trên phương pháp DerSimonian-Laird. Kết quả Trong số những bệnh nhân bị đau ngực do cocaine hoặc sử dụng cocaine gần đây, không có sự khác biệt đáng kể về tỷ lệ tử vong toàn nguyên nhân trong bệnh viện (RR, 0,59; KTC 95%, 0,24 - 1,47) và MI (RR, 1,24; KTC 95%, 0,74 - 2,06) giữa những bệnh nhân có và không được điều trị bằng thuốc chẹn β trong thời gian nằm viện. Trong quá trình theo dõi dài hạn (trung bình 2,6 năm), không có sự khác biệt đáng kể về tỷ lệ tử vong toàn nguyên nhân (RR, 0,79; KTC 95%, 0,44 - 1,41) và MI (RR, 0,96; KTC 95%, 0,40 - 2,33) giữa hai nhóm. Kết luận Những kết quả này cho thấy việc điều trị bằng thuốc chẹn β ở những bệnh nhân bị ngộ độc cocaine có thể không gây hại như ban đầu vẫn tin. Cần tiến hành thêm các nghiên cứu lâm sàng để điều tra chủ đề này."} +{"idx": 1910133, "en": "Atrial tachycardias (ATs) are relatively uncommon and their mechanisms include reentry or may be focal. The characteristics and radiofrequency (RF) ablation of adenosine-sensitive AT arising near the apex of Koch's triangle have been reported. We report a case of successful RF ablation of this tachycardia by using a retrograde transaortic/transmitral approach. An electrophysiological study performed during sinus rhythm showed continuous anterograde atrioventricular nodal and no ventriculoatrial conduction. The tachycardia could be induced and terminated by atrial extrastimulation and rapid atrial pacing. Although inverse resetting response pattern was observed, tachycardia termination by intravenous low-dose adenosine was rather suggestive of triggered activity as the mechanism. The earliest atrial activation was recorded in the His-bundle region. After ineffective right-sided attempts and failure to find earlier atrial activity during mapping the noncoronary sinus of Valsalva, this tachycardia was successfully ablated from the left atrial septum by using a retrograde transaortic/transmitral approach. This report demonstrates the feasibility of a novel retrograde left atrial approach for RF ablation of adenosine-sensitive AT originating from the apex of Koch's triangle.", "gemini": "Nhịp tim nhanh trên thất (AT) tương đối ít gặp và cơ chế của chúng bao gồm tái nhập hoặc có thể là ổ. Các đặc điểm và tiêu hủy bằng tần số vô tuyến (RF) của AT nhạy cảm với adenosine phát sinh gần đỉnh tam giác Koch đã được báo cáo. Chúng tôi báo cáo một trường hợp tiêu hủy bằng RF thành công nhịp tim nhanh này bằng cách sử dụng phương pháp xuyên động mạch chủ ngược dòng / xuyên van hai lá. Một nghiên cứu điện sinh lý được thực hiện trong nhịp xoang cho thấy nút nhĩ thất hướng trước liên tục và không có dẫn truyền thất nhĩ. Nhịp tim nhanh có thể được gây ra và chấm dứt bằng kích thích điện ngoài nhĩ và nhịp nhanh nhĩ. Mặc dù quan sát thấy kiểu phản ứng đặt lại ngược, nhưng việc chấm dứt nhịp tim nhanh bằng adenosine liều thấp tiêm tĩnh mạch gợi ý nhiều hơn về hoạt động kích hoạt như cơ chế. Hoạt động nhĩ sớm nhất được ghi lại ở vùng bó His. Sau những nỗ lực không hiệu quả ở bên phải và không tìm thấy hoạt động nhĩ sớm hơn trong quá trình lập bản đồ xoang Valsalva không có động mạch vành, nhịp tim nhanh này đã được tiêu hủy thành công từ vách nhĩ trái bằng cách sử dụng phương pháp xuyên động mạch chủ ngược dòng / xuyên van hai lá. Báo cáo này chứng minh tính khả thi của một phương pháp tiếp cận nhĩ trái ngược dòng mới để tiêu hủy bằng RF AT nhạy cảm với adenosine có nguồn gốc từ đỉnh tam giác Koch."} +{"idx": 1910134, "en": "A 42-year-old hypertensive woman came to our hospital suffering from shortness of breath. Her left ventricular mass index (LVMI) was increased, and a new arterial stiffness index, cardio-ankle vascular index (CAVI), was also elevated. By treating hypertension (HT), diabetes mellitus (DM) and obstructive sleep apnea (OSA), her left ventricular concentric hypertrophy was improved, accompanying with a decrease in CAVI. These observations suggested that arterial stiffness monitored with CAVI might be involved in cardiac hypertrophy. This cardio-vascular interaction could be demonstrated at the first time by monitoring CAVI, which is not affected by blood pressure (BP) at measuring time.", "gemini": "Một phụ nữ tăng huyết áp 42 tuổi đến bệnh viện của chúng tôi vì bị khó thở. Chỉ số khối lượng thất trái (LVMI) của cô ấy tăng lên và chỉ số độ cứng động mạch mới, chỉ số mạch máu mắt cá chân tim (CAVI), cũng tăng lên. Bằng cách điều trị tăng huyết áp (HT), đái tháo đường (DM) và ngưng thở khi ngủ tắc nghẽn (OSA), chứng phì đại đồng tâm thất trái của cô ấy đã được cải thiện, đi kèm với sự giảm CAVI. Những quan sát này cho thấy độ cứng động mạch được theo dõi bằng CAVI có thể liên quan đến phì đại tim. Tương tác tim mạch này có thể được chứng minh lần đầu tiên bằng cách theo dõi CAVI, không bị ảnh hưởng bởi huyết áp (BP) tại thời điểm đo."} +{"idx": 1910135, "en": "Inflammation of the myocardium (myocarditis) or pericardium (pericarditis) or both (myopericarditis) as side effects of mesalamine, a drug widely used in the treatment of inflammatory bowel disease, is a rare, but potentially lethal complication. We report a case of myopericarditis occurring in a young Caucasian woman 14 days following initiation of mesalamine therapy for treatment of a newly diagnosed ulcerative colitis (UC). She presented with pleuritic chest pain, elevated troponin levels and pre-syncope. The diagnosis of myopericarditis was made based on the clinical features, electrocardiogram (EKG) and cardiac magnetic resonance, which showed trace pericardial effusion. The patient's symptom and condition were dramatically improved upon discontinuing mesalamine, and a full recovery was achieved. Mesalamine-induced inflammation of the myocardium (myocarditis) or pericardium (pericarditis) or both (myopericarditis) is rare, but has fatal side effects. Early recognition of these side effects by clinicians and patients is important to prevent progression of the inflammation. Furthermore, patients should be educated to seek urgent medical attention if cardiac symptoms arise.", "gemini": "Viêm cơ tim (viêm cơ tim) hoặc viêm màng ngoài tim (viêm màng ngoài tim) hoặc cả hai (viêm cơ tim ngoài tim) là tác dụng phụ của mesalamine, một loại thuốc được sử dụng rộng rãi trong điều trị bệnh viêm ruột, là một biến chứng hiếm gặp nhưng có khả năng gây tử vong. Chúng tôi báo cáo một trường hợp viêm cơ tim ngoài tim xảy ra ở một phụ nữ da trắng trẻ tuổi 14 ngày sau khi bắt đầu điều trị bằng mesalamine để điều trị viêm loét đại tràng mới được chẩn đoán (UC). Cô ấy xuất hiện với cơn đau ngực do màng phổi, mức troponin tăng cao và tiền ngất. Chẩn đoán viêm cơ tim ngoài tim được đưa ra dựa trên các đặc điểm lâm sàng, điện tâm đồ (EKG) và cộng hưởng từ tim, cho thấy dấu vết tràn dịch màng ngoài tim. Các triệu chứng và tình trạng của bệnh nhân được cải thiện đáng kể sau khi ngừng dùng mesalamine và đã phục hồi hoàn toàn. Viêm cơ tim (viêm cơ tim) hoặc viêm màng ngoài tim (viêm màng ngoài tim) hoặc cả hai (viêm cơ tim ngoài tim) do mesalamine gây ra rất hiếm, nhưng có tác dụng phụ gây tử vong. Việc các bác sĩ lâm sàng và bệnh nhân nhận biết sớm các tác dụng phụ này rất quan trọng để ngăn ngừa tình trạng viêm tiến triển. Hơn nữa, bệnh nhân nên được giáo dục để tìm kiếm sự chăm sóc y tế khẩn cấp nếu các triệu chứng về tim xuất hiện."} +{"idx": 1910136, "en": "A paradoxical embolism is defined as a systemic arterial embolus due to passage of a venous thrombus through a right to left shunt. In recent years, vitamin K antagonists and aspirin are used as anticoagulant medications for the secondary prevention of paradoxical embolism. We describe a case of subacute right upper limb ischemia due to paradoxical embolism. We first started treatment with urokinase, intravenous (IV) injection and unfractionated heparin (continuous IV). As her condition and the serum D-dimer level showed improvement, we started catheterization on day 7 after admission; however the right brachial artery thrombus did not disappear. For her outpatient care, based on the viewpoint of providing rapid anticoagulation therapy within the therapeutic range, having longest periods of initial intensive therapy we chose the treatment using ribaroxaban. Recanalization of her right brachial artery was achieved with this therapy. Here, we report the effective results of initial intensive therapy using ribaroxaban for paradoxical embolism.", "gemini": "Thuyên tắc nghịch thường được định nghĩa là thuyên tắc động mạch toàn thân do cục máu đông tĩnh mạch đi qua một đường thông từ phải sang trái. Trong những năm gần đây, thuốc đối kháng vitamin K và aspirin được sử dụng làm thuốc chống đông để phòng ngừa thứ phát thuyên tắc nghịch thường. Chúng tôi mô tả một trường hợp thiếu máu chi trên phải bán cấp do thuyên tắc nghịch thường. Đầu tiên, chúng tôi bắt đầu điều trị bằng urokinase, tiêm tĩnh mạch (IV) và heparin không phân đoạn (IV liên tục). Khi tình trạng của cô ấy và mức D-dimer trong huyết thanh cho thấy có sự cải thiện, chúng tôi bắt đầu đặt ống thông vào ngày thứ 7 sau khi nhập viện; tuy nhiên, cục máu đông ở động mạch cánh tay phải không biến mất. Đối với việc chăm sóc ngoại trú của cô ấy, dựa trên quan điểm cung cấp liệu pháp chống đông nhanh trong phạm vi điều trị, có thời gian điều trị tích cực ban đầu dài nhất, chúng tôi đã chọn phương pháp điều trị bằng ribaroxaban. Tái thông động mạch cánh tay phải của cô ấy đã đạt được với liệu pháp này. Ở đây, chúng tôi báo cáo kết quả hiệu quả của liệu pháp tích cực ban đầu sử dụng ribaroxaban đối với thuyên tắc nghịch thường."} +{"idx": 1910137, "en": "Adult-onset leukoencephalopathy with axonal spheroids and pigmented glia (ALSP) is a rare neurodegenerative disorder characterized by cerebral white matter abnormalities, myelin loss, and axonal swellings. ALSP is caused by mutations in colony stimulating factor 1 receptor gene. We report an ASLP patient with asymptomatic intracranial calcifications distributed in white matter found incidentally in a health screening.", "gemini": "Bệnh não thoái hóa khởi phát ở người lớn với các cầu sợi trục và tế bào thần kinh đệm có sắc tố (ALSP) là một rối loạn thoái hóa thần kinh hiếm gặp được đặc trưng bởi các bất thường ở chất trắng não, mất myelin và sưng trục. ALSP là do đột biến ở gen thụ thể yếu tố kích thích khuẩn lạc 1. Chúng tôi báo cáo một bệnh nhân ASLP với các vôi hóa nội sọ không triệu chứng phân bố ở chất trắng được phát hiện tình cờ trong một cuộc kiểm tra sức khỏe."} +{"idx": 1910138, "en": "Phyllodes tumors are rare neoplasms that account for 2.5% of all fibroepithelial lesions, and 10%-20% exhibit malignant transformation. Malignant transformation often occurs in the form of stroma, and usually shows fibrosarcomatous differentiation. Liposarcomatous differentiation is a rare, developed stromal component of phyllodes tumors, and little is known about their imaging findings. We present the case of a 47-year-old woman who was diagnosed with a malignant phyllodes tumor of the breast that contained liposarcomatous elements. The patient underwent wide surgical excision of the mass and has been treated with adjuvant radiation therapy.", "gemini": "U phyllodes là khối u tân sinh hiếm gặp chiếm 2,5% trong tất cả các tổn thương biểu mô sợi, và 10% - 20% biểu hiện chuyển dạng ác tính. Chuyển dạng ác tính thường xảy ra ở dạng mô đệm và thường biểu hiện biệt hóa thành sợi sarcoma. Biệt hóa thành mỡ là một thành phần mô đệm hiếm gặp, phát triển của u phyllodes và người ta biết rất ít về các phát hiện hình ảnh của chúng. Chúng tôi trình bày trường hợp một phụ nữ 47 tuổi được chẩn đoán mắc u phyllodes ác tính ở vú có chứa các yếu tố mỡ. Bệnh nhân đã được cắt bỏ khối u rộng và được điều trị bằng xạ trị bổ trợ."} +{"idx": 1910139, "en": "Hepatocellular carcinoma is the most frequent type of liver malignancy. Most cases of hepatocellular carcinoma are secondary to either viral hepatitis (hepatitis B, C) or alcoholic cirrhosis. Liver cirrhosis due to any other causes is considered as a risk factor for development of hepatocellular carcinoma; however, hepatocellular carcinoma in a young patient with no cirrhosis or fibrosis is a relatively rare condition. The present case report describes a young 24-years-old male patient who presented with a two-month history of fever, right upper quadrant abdominal pain, and weight loss with no evidence of pre-existing liver disease, initially treated as liver abscess; however, a computed tomography (CT) showed numerous hepatic hypodensities with ring enhancement. Serum alpha-fetoprotein level was high (>1000 ng/L) and histopathological study confirmed the presence of primary hepatocellular carcinoma. The present case findings suggest that multifocal hepatocellular carcinoma can occur in a young patient with no apparent risk factor, and that the clinical presentation of hepatocellular carcinoma can be similar to liver abscess.", "gemini": "Ung thư biểu mô tế bào gan là loại ung thư gan phổ biến nhất. Hầu hết các trường hợp ung thư biểu mô tế bào gan là thứ phát do viêm gan do vi-rút (viêm gan B, C) hoặc xơ gan do rượu. Xơ gan do bất kỳ nguyên nhân nào khác được coi là yếu tố nguy cơ phát triển ung thư biểu mô tế bào gan; tuy nhiên, ung thư biểu mô tế bào gan ở bệnh nhân trẻ không bị xơ gan hoặc xơ hóa là một tình trạng tương đối hiếm gặp. Báo cáo trường hợp hiện tại mô tả một bệnh nhân nam 24 tuổi trẻ tuổi có tiền sử sốt hai tháng, đau bụng ở góc phần tư trên bên phải và sụt cân mà không có bằng chứng về bệnh gan từ trước, ban đầu được điều trị như áp xe gan; tuy nhiên, chụp cắt lớp vi tính (CT) cho thấy nhiều vùng giảm mật độ ở gan với tăng cường vành. Nồng độ alpha-fetoprotein trong huyết thanh cao (> 1000 ng / L) và nghiên cứu mô học xác nhận sự hiện diện của ung thư biểu mô tế bào gan nguyên phát. Những phát hiện của trường hợp hiện tại cho thấy ung thư biểu mô tế bào gan đa ổ có thể xảy ra ở bệnh nhân trẻ không có yếu tố nguy cơ rõ ràng và biểu hiện lâm sàng của ung thư biểu mô tế bào gan có thể tương tự như áp xe gan."} +{"idx": 1910140, "en": "This is a case of bilateral cavernous sinus thrombosis in a five-and-a-half-year-old boy who presented with bilateral proptosis with difficulty in swallowing and excessive salivation. Clinical features along with contrast-enhanced computed tomography and hemogram diagnosed the case as bilateral cavernous sinus thrombosis and retropharyngeal abscess with third cranial nerve palsy. The patient was treated with standard regimen successfully. Cavernous sinus thrombosis is a very rare complication of retropharyngeal abscess.", "gemini": "Đây là trường hợp huyết khối xoang hang hai bên ở một bé trai năm tuổi rưỡi có biểu hiện lồi mắt hai bên với khó nuốt và chảy nước dãi quá mức. Các đặc điểm lâm sàng cùng với chụp cắt lớp vi tính có tiêm thuốc cản quang và xét nghiệm máu chẩn đoán trường hợp này là huyết khối xoang hang hai bên và áp xe sau họng với liệt dây thần kinh sọ não thứ ba. Bệnh nhân đã được điều trị theo phác đồ chuẩn thành công. Huyết khối xoang hang là một biến chứng rất hiếm gặp của áp xe sau họng."} +{"idx": 1910141, "en": "Snake bites are an important public health problem worldwide. Snake venom causes both systemic and local complications, which can prove fatal if not treated on time. The local effects of snake bite include tissue necrosis, edema, and compartment syndrome. Patients may also be left with permanent physical deformities due to residual sequelae of the snake bite. Compartment syndrome after a snake bite is an uncommon occurrence. The effects are more pronounced in children possibly due to the the reduced total dilution volume in children. The administration of anti-snake venom is the only specific therapy. Compartment syndrome occurs due to a vicious cycle of edema causing hypoxia and acidosis, which further increases capillary permeability and fluid extravasation. This results in a volume increase in the closed fascial compartment, which ultimately compromises circulation and causes irreversible muscle and nerve damage. Our report describes a case of upper limb compartment syndrome following a snake bite on the right wrist of a five-year-old girl who presented eight-hours after the snake bite to the emergency department of Nizwa Regional Referral Hospital. The patient received early and appropriate care but progressed to develop compartment syndrome for which she had to be taken to the operating theatre for emergency fasciotomy. All clinicians should be able to recognize the early symptoms and signs of an evolving compartment syndrome in absence of intracompartmental measuring equipment. The timely fasciotomy in our patient helped the patient achieve excellent functional results.", "gemini": "Vết rắn cắn là một vấn đề sức khỏe cộng đồng quan trọng trên toàn thế giới. Nọc rắn gây ra cả biến chứng toàn thân và tại chỗ, có thể gây tử vong nếu không được điều trị kịp thời. Các tác động tại chỗ của vết rắn cắn bao gồm hoại tử mô, phù nề và hội chứng khoang. Bệnh nhân cũng có thể bị dị dạng thể chất vĩnh viễn do di chứng còn lại của vết rắn cắn. Hội chứng khoang sau khi bị rắn cắn là một tình trạng không phổ biến. Các tác động này rõ rệt hơn ở trẻ em, có thể là do thể tích pha loãng toàn phần ở trẻ em giảm. Tiêm huyết thanh kháng nọc rắn là phương pháp điều trị đặc hiệu duy nhất. Hội chứng khoang xảy ra do một vòng luẩn quẩn của phù nề gây thiếu oxy và nhiễm toan, làm tăng thêm tính thấm của mao mạch và thoát dịch. Điều này dẫn đến tăng thể tích trong khoang cân mạc kín, cuối cùng làm tổn thương tuần hoàn và gây tổn thương cơ và thần kinh không hồi phục. Báo cáo của chúng tôi mô tả một trường hợp hội chứng khoang chi trên sau khi bị rắn cắn vào cổ tay phải của một bé gái năm tuổi đến khoa cấp cứu của Bệnh viện Chuyển tuyến Khu vực Nizwa sau tám giờ bị rắn cắn. Bệnh nhân đã được chăm sóc sớm và phù hợp nhưng tiến triển thành hội chứng khoang, phải đưa vào phòng phẫu thuật để cắt cân cấp cứu. Tất cả các bác sĩ lâm sàng đều có thể nhận biết các triệu chứng và dấu hiệu sớm của hội chứng khoang tiến triển khi không có thiết bị đo lường bên trong khoang. Phẫu thuật cắt cân kịp thời cho bệnh nhân của chúng tôi đã giúp bệnh nhân đạt được kết quả chức năng tuyệt vời."} +{"idx": 1910142, "en": "Introduction: The human oral microbiota continues to change phenotype by many factors (environment, diet, genetics, stress, etc.), throughout life with a major impact on human physiology, psychology, metabolism and immune system. Amongst one such factor with unique and extreme environmental conditions is Antarctica. The sea voyage to Antarctica has many risks than at station for expedition members. In this study, we investigated the influence of Antarctic sea voyage and stay at the Indian Antarctic station Maitri, on the health of Indian expedition members by using a metagenomic approach to explore oral biodiversity. Methods: Saliva samples were collected from 12 expedition members, at 3 different time points viz. before the start of the ship voyage, after the completion of the voyage and at the end of the stay at Antarctica. Samples were analyzed for whole genome and 16S rRNA sequencing. Result: The oral microbial diversity of the expedition members was significantly changed, during the days of sailing and after the stay at Antarctica. The oral microbiota comprised mainly of the phyla Firmicutes (46%, 29% & 36%); Proteobacteria (40%, 48%, & 44%), Bacteroidetes (10%, 22%, &14%), Fusobacterium and Actinobacteria (5%-1%) and Unclassified (17%, 25% & 23%), at three time points, respectively. Further, the differential analysis of microbes across all the phyla revealed 89, 157 and 157 OTUs genera. The altered microbiota indicated changes in amino acid, lipid and carbohydrate metabolism. Conclusion: Study suggests that understanding the compositional and functional differences in the oral microbiota of Antarctic expedition members, can lay the foundation to relate these differences to their health status. It will further demonstrate the need for providing improved management during ship voyage and stay in Antarctica.", "gemini": "Giới thiệu: Hệ vi sinh vật trong khoang miệng của con người liên tục thay đổi kiểu hình do nhiều yếu tố (môi trường, chế độ ăn uống, di truyền, căng thẳng, v.v.) trong suốt cuộc đời với tác động lớn đến sinh lý, tâm lý, quá trình trao đổi chất và hệ miễn dịch của con người. Nam Cực là một trong những yếu tố như vậy với điều kiện môi trường độc đáo và khắc nghiệt. Chuyến đi trên biển đến Nam Cực có nhiều rủi ro hơn tại trạm đối với các thành viên của đoàn thám hiểm. Trong nghiên cứu này, chúng tôi đã điều tra ảnh hưởng của chuyến đi trên biển Nam Cực và thời gian lưu trú tại trạm Nam Cực Ấn Độ Maitri đối với sức khỏe của các thành viên đoàn thám hiểm Ấn Độ bằng cách sử dụng phương pháp tiếp cận metagenomic để khám phá sự đa dạng sinh học trong khoang miệng. Phương pháp: Các mẫu nước bọt được thu thập từ 12 thành viên của đoàn thám hiểm, tại 3 thời điểm khác nhau, cụ thể là trước khi bắt đầu chuyến đi trên tàu, sau khi hoàn thành chuyến đi và vào cuối thời gian lưu trú tại Nam Cực. Các mẫu được phân tích để giải trình tự toàn bộ hệ gen và rRNA 16S. Kết quả: Sự đa dạng của vi khuẩn trong khoang miệng của các thành viên đoàn thám hiểm đã thay đổi đáng kể trong những ngày đi biển và sau thời gian lưu trú tại Nam Cực. Hệ vi sinh vật trong khoang miệng chủ yếu bao gồm các ngành Firmicutes (46%, 29% & 36%); Proteobacteria (40%, 48%, & 44%), Bacteroidetes (10%, 22%, & 14%), Fusobacterium và Actinobacteria (5% -1%) và Không được phân loại (17%, 25% & 23%), tương ứng tại ba thời điểm. Hơn nữa, phân tích khác biệt của vi khuẩn trên tất cả các ngành đã tiết lộ 89, 157 và 157 chi OTU. Hệ vi sinh vật thay đổi cho thấy sự thay đổi trong quá trình trao đổi chất axit amin, lipid và carbohydrate. Kết luận: Nghiên cứu cho thấy rằng việc hiểu được sự khác biệt về thành phần và chức năng trong hệ vi sinh vật trong khoang miệng của các thành viên đoàn thám hiểm Nam Cực có thể đặt nền tảng để liên hệ những khác biệt này với tình trạng sức khỏe của họ. Điều này sẽ chứng minh thêm nhu cầu cung cấp dịch vụ quản lý được cải thiện trong suốt chuyến đi trên tàu và thời gian lưu trú tại Nam Cực."} +{"idx": 1910143, "en": "Background: Elevated prevalences of post-traumatic stress disorder (PTSD) and major depression (MD) have been reported in populations exposed to war. However, no global estimates of war survivors suffering from PTSD and/or MD in absolute numbers have been reported. Objective: We made the first attempt to estimate in absolute numbers how many adult war survivors globally may suffer PTSD and/or MD, which should inform local and global mental health programmes. Method: Drawing on the Uppsala Conflict Database, we reviewed all countries that suffered at least one war within their own territory between 1989 and 2015 (time span chosen on availability of geo-referenced data and population estimates). We then conducted a meta-analysis of current randomized epidemiological surveys on prevalence of PTSD and/or MD among war survivors. Finally, we extrapolated our results from the meta-analysis on the global population of adult war survivors by means of using general population data from the United Nations. Results: We estimate that about 1.45 billion individuals worldwide have experienced war between 1989 and 2015 and were still alive in 2015, including one billion adults. On the basis of our meta-analysis, we estimate that about 354 million adult war survivors suffer from PTSD and/or MD. Of these, about 117 million suffer from comorbid PTSD and MD. Conclusions: Based on the slim available evidence base, the global number of adult war survivors suffering PTSD and/or MD is vast. Most war survivors live in low-to-middle income countries with limited means to handle the enormous mental health burden. Since representative high quality data is lacking from most of these countries, our results contain a large margin of uncertainty and should be interpreted with caution.", "gemini": "Bối cảnh: Tỷ lệ mắc hội chứng căng thẳng sau chấn thương (PTSD) và trầm cảm chính (MD) gia tăng đã được báo cáo ở những người dân phải hứng chịu chiến tranh. Tuy nhiên, vẫn chưa có ước tính toàn cầu nào về những người sống sót sau chiến tranh mắc PTSD và/hoặc MD theo số lượng tuyệt đối. Mục tiêu: Chúng tôi đã thực hiện nỗ lực đầu tiên để ước tính theo số lượng tuyệt đối có bao nhiêu người lớn sống sót sau chiến tranh trên toàn cầu có thể mắc PTSD và/hoặc MD, điều này sẽ cung cấp thông tin cho các chương trình sức khỏe tâm thần tại địa phương và toàn cầu. Phương pháp: Dựa trên Cơ sở dữ liệu xung đột Uppsala, chúng tôi đã xem xét tất cả các quốc gia phải hứng chịu ít nhất một cuộc chiến tranh trong lãnh thổ của mình từ năm 1989 đến năm 2015 (khoảng thời gian được chọn dựa trên tính khả dụng của dữ liệu tham chiếu địa lý và ước tính dân số). Sau đó, chúng tôi đã tiến hành phân tích tổng hợp các cuộc điều tra dịch tễ học ngẫu nhiên hiện tại về tỷ lệ mắc PTSD và/hoặc MD ở những người sống sót sau chiến tranh. Cuối cùng, chúng tôi đã ngoại suy kết quả của mình từ phân tích tổng hợp về dân số toàn cầu của những người lớn sống sót sau chiến tranh bằng cách sử dụng dữ liệu dân số chung từ Liên hợp quốc. Kết quả: Chúng tôi ước tính rằng khoảng 1,45 tỷ cá nhân trên toàn thế giới đã trải qua chiến tranh trong giai đoạn 1989-2015 và vẫn còn sống vào năm 2015, trong đó có một tỷ người lớn. Trên cơ sở phân tích tổng hợp của mình, chúng tôi ước tính rằng khoảng 354 triệu người lớn sống sót sau chiến tranh mắc PTSD và/hoặc MD. Trong số những người này, khoảng 117 triệu người mắc PTSD và MD mắc kèm. Kết luận: Dựa trên cơ sở dữ liệu có sẵn ít ỏi, số lượng người lớn sống sót sau chiến tranh trên toàn cầu mắc PTSD và/hoặc MD là rất lớn. Hầu hết những người sống sót sau chiến tranh sống ở các quốc gia có thu nhập thấp đến trung bình với phương tiện hạn chế để giải quyết gánh nặng sức khỏe tâm thần to lớn. Vì thiếu dữ liệu chất lượng cao có tính đại diện từ hầu hết các quốc gia này, nên kết quả của chúng tôi có biên độ không chắc chắn lớn và cần được hiểu một cách thận trọng."} diff --git a/retranslated_vi_pubmed/chunk_191/error.json b/retranslated_vi_pubmed/chunk_191/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_191/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_192/completed.json b/retranslated_vi_pubmed/chunk_192/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..c9ca6473a5ecec8f0d30896d49ef5189d668ab73 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_192/completed.json @@ -0,0 +1,146 @@ +[ + 1920000, + 1920001, + 1920002, + 1920003, + 1920004, + 1920005, + 1920006, + 1920007, + 1920008, + 1920009, + 1920010, + 1920011, + 1920012, + 1920013, + 1920014, + 1920015, + 1920016, + 1920017, + 1920018, + 1920019, + 1920020, + 1920021, + 1920022, + 1920023, + 1920024, + 1920025, + 1920026, + 1920027, + 1920028, + 1920029, + 1920030, + 1920031, + 1920032, + 1920033, + 1920034, + 1920035, + 1920036, + 1920037, + 1920038, + 1920039, + 1920040, + 1920041, + 1920042, + 1920043, + 1920044, + 1920045, + 1920046, + 1920047, + 1920048, + 1920049, + 1920050, + 1920051, + 1920052, + 1920053, + 1920054, + 1920055, + 1920056, + 1920057, + 1920058, + 1920059, + 1920060, + 1920061, + 1920062, + 1920063, + 1920064, + 1920065, + 1920066, + 1920067, + 1920068, + 1920069, + 1920070, + 1920071, + 1920072, + 1920073, + 1920074, + 1920075, + 1920076, + 1920077, + 1920078, + 1920079, + 1920080, + 1920081, + 1920082, + 1920083, + 1920084, + 1920085, + 1920086, + 1920087, + 1920088, + 1920089, + 1920090, + 1920091, + 1920092, + 1920093, + 1920094, + 1920095, + 1920096, + 1920097, + 1920098, + 1920099, + 1920100, + 1920101, + 1920102, + 1920103, + 1920104, + 1920105, + 1920106, + 1920107, + 1920108, + 1920109, + 1920110, + 1920111, + 1920112, + 1920113, + 1920114, + 1920115, + 1920116, + 1920117, + 1920118, + 1920119, + 1920120, + 1920121, + 1920122, + 1920123, + 1920124, + 1920125, + 1920126, + 1920127, + 1920128, + 1920129, + 1920130, + 1920131, + 1920132, + 1920133, + 1920134, + 1920135, + 1920136, + 1920137, + 1920138, + 1920139, + 1920140, + 1920141, + 1920142, + 1920143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_192/data.jsonl b/retranslated_vi_pubmed/chunk_192/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..e75511fd78ca02ab7482f80bc7f2274bf5498eb3 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_192/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1920000, "en": "In this paper, we introduce fuzzy theory into the fractional cellular neural networks to dynamically enhance the coupling strength and propose a fractional fuzzy neural network model with interactions. Using the Lyapunov principle of fractional differential equations, we design the adaptive control schemes to realize the synchronization and obtain the synchronization criteria. Finally, we provide some numerical examples to show the effectiveness of our obtained results.", "gemini": "Trong bài báo này, chúng tôi giới thiệu lý thuyết mờ vào mạng lưới nơ-ron thần kinh phân số để tăng cường động lực sức mạnh liên kết và đề xuất một mô hình mạng lưới nơ-ron thần kinh mờ phân số với các tương tác. Sử dụng nguyên lý Lyapunov của các phương trình vi phân phân số, chúng tôi thiết kế các lược đồ điều khiển thích ứng để hiện thực hóa đồng bộ hóa và thu được các tiêu chuẩn đồng bộ hóa. Cuối cùng, chúng tôi cung cấp một số ví dụ số để chứng minh hiệu quả của các kết quả thu được của chúng tôi."} +{"idx": 1920001, "en": "In this paper, we propose a simple quantum model of the kaons decay providing an estimate of the CP symmetry violation parameter. We use the two-level Friedrich's Hamiltonian model to obtain a good quantitative agreement with the experimental estimate of the violation parameter for neutral kaons. A temporal wave-function approach, based on an analogy with spatial wave-functions, plays a crucial role in our model.", "gemini": "Trong bài báo này, chúng tôi đề xuất một mô hình lượng tử đơn giản về sự phân rã kaon cung cấp ước tính tham số vi phạm đối xứng CP. Chúng tôi sử dụng mô hình Hamiltonian Friedrich hai mức để có được sự đồng thuận định lượng tốt với ước tính thực nghiệm về tham số vi phạm đối với kaon trung hòa. Một phương pháp tiếp cận hàm sóng thời gian, dựa trên sự tương tự với các hàm sóng không gian, đóng một vai trò quan trọng trong mô hình của chúng tôi."} +{"idx": 1920002, "en": "We present a collective coordinate approach to study the collective behaviour of a finite ensemble of N stochastic Kuramoto oscillators using two degrees of freedom: one describing the shape dynamics of the oscillators and one describing their mean phase. Contrary to the thermodynamic limit N → ∞ in which the mean phase of the cluster of globally synchronized oscillators is constant in time, the mean phase of a finite-size cluster experiences Brownian diffusion with a variance proportional to 1/N. This finite-size effect is quantitatively well captured by our collective coordinate approach.", "gemini": "Chúng tôi trình bày một phương pháp tiếp cận tọa độ tập thể để nghiên cứu hành vi tập thể của một tập hợp hữu hạn N dao động ngẫu nhiên Kuramoto sử dụng hai bậc tự do: một mô tả động lực hình dạng của các dao động và một mô tả pha trung bình của chúng. Trái ngược với giới hạn nhiệt động N → ∞ trong đó pha trung bình của cụm các dao động đồng bộ toàn cục không đổi theo thời gian, pha trung bình của một cụm kích thước hữu hạn trải qua khuếch tán Brown với phương sai tỷ lệ với 1/N. Hiệu ứng kích thước hữu hạn này được phương pháp tiếp cận tọa độ tập thể của chúng tôi nắm bắt tốt về mặt định lượng."} +{"idx": 1920003, "en": "Hydrodynamic flows can exert multiple effects on an exothermal autocatalytic reaction, such as buoyancy and the Marangoni convection, which can change the structure and velocity of chemical waves. Here we report that in the chlorite-trithionate reaction, the production and consumption of chlorine dioxide can induce and inhibit Marangoni flow, respectively, leading to different chemo-hydrodynamic patterns. The horizontal propagation of a reaction-diffusion-convection front was investigated with the upper surface open to the air. The Marangoni convection, induced by gaseous chlorine dioxide on the surface, produced from chlorite disproportionation after the proton autocatalysis, has the same effect as the heat convection. When the Marangoni effect is removed by the reaction of chlorine dioxide with the Congo red (CR) indicator, an oscillatory propagation of the front tip is observed under suitable conditions. Replacing CR with bromophenol blue (BPB) distinctly enhanced the floating, resulting in multiple vortexes, owing to the coexistence between BPB and chlorine dioxide. Using the incompressible Navier-Stokes equations coupled with reaction-diffusion and heat conduction equations, we numerically obtain various experimental scenarios of front instability for the exothermic autocatalytic reaction coupled with buoyancy-driven convection and Marangoni convection.", "gemini": "Dòng chảy thủy động lực có thể gây ra nhiều tác động lên phản ứng tự xúc tác tỏa nhiệt, chẳng hạn như lực đẩy và đối lưu Marangoni, có thể làm thay đổi cấu trúc và vận tốc của sóng hóa học. Ở đây, chúng tôi báo cáo rằng trong phản ứng chlorit-trithionat, quá trình sản xuất và tiêu thụ clo dioxit có thể lần lượt gây ra và ức chế dòng chảy Marangoni, dẫn đến các kiểu hóa học-thủy động lực học khác nhau. Sự lan truyền theo chiều ngang của mặt trận phản ứng-khuếch tán-đối lưu đã được nghiên cứu với bề mặt trên mở ra không khí. Đối lưu Marangoni do clo dioxit khí trên bề mặt tạo ra từ sự phân hủy không cân đối của chlorit sau khi tự xúc tác proton, có tác dụng giống như đối lưu nhiệt. Khi hiệu ứng Marangoni bị loại bỏ do phản ứng của clo dioxit với chỉ thị đỏ Congo (CR), người ta quan sát thấy sự lan truyền dao động của đầu mặt trận trong các điều kiện thích hợp. Thay thế CR bằng xanh bromphenol (BPB) làm tăng rõ rệt sự nổi, dẫn đến nhiều xoáy, do sự cùng tồn tại giữa BPB và clo dioxit. Sử dụng các phương trình Navier-Stokes không nén được kết hợp với các phương trình phản ứng-khuếch tán và dẫn nhiệt, chúng tôi thu được số các kịch bản thực nghiệm khác nhau về sự không ổn định của mặt trận đối với phản ứng tự xúc tác tỏa nhiệt kết hợp với đối lưu do lực đẩy và đối lưu Marangoni."} +{"idx": 1920004, "en": "Observability is the property that enables recovering the state of a dynamical system from a reduced number of measured variables. In high-dimensional systems, it is therefore important to make sure that the variable recorded to perform the analysis conveys good observability of the system dynamics. The observability of a network of neuron models depends nontrivially on the observability of the node dynamics and on the topology of the network. The aim of this paper is twofold. First, to perform a study of observability using four well-known neuron models by computing three different observability coefficients. This not only clarifies observability properties of the models but also shows the limitations of applicability of each type of coefficients in the context of such models. Second, to study the emergence of phase synchronization in networks composed of neuron models. This is done performing multivariate singular spectrum analysis which, to the best of the authors' knowledge, has not been used in the context of networks of neuron models. It is shown that it is possible to detect phase synchronization: (i) without having to measure all the state variables, but only one (that provides greatest observability) from each node and (ii) without having to estimate the phase.", "gemini": "Khả năng quan sát là đặc tính cho phép khôi phục trạng thái của một hệ thống động lực từ một số biến đo được giảm. Do đó, trong các hệ thống có chiều cao, điều quan trọng là phải đảm bảo rằng biến được ghi lại để thực hiện phân tích truyền tải khả năng quan sát tốt về động lực hệ thống. Khả năng quan sát của một mạng lưới các mô hình nơ-ron phụ thuộc không tầm thường vào khả năng quan sát của động lực nút và vào cấu trúc mạng lưới. Mục đích của bài báo này có hai mặt. Đầu tiên, thực hiện một nghiên cứu về khả năng quan sát bằng cách sử dụng bốn mô hình nơ-ron nổi tiếng bằng cách tính toán ba hệ số khả năng quan sát khác nhau. Điều này không chỉ làm rõ các đặc tính khả năng quan sát của các mô hình mà còn cho thấy những hạn chế về khả năng áp dụng của từng loại hệ số trong bối cảnh của các mô hình như vậy. Thứ hai, nghiên cứu sự xuất hiện của đồng bộ pha trong các mạng lưới được tạo thành từ các mô hình nơ-ron. Điều này được thực hiện bằng cách thực hiện phân tích phổ kỳ dị đa biến, theo hiểu biết tốt nhất của các tác giả, chưa được sử dụng trong bối cảnh của các mạng lưới mô hình nơ-ron. Người ta chỉ ra rằng có thể phát hiện đồng bộ pha: (i) mà không cần phải đo tất cả các biến trạng thái, mà chỉ cần một biến (cung cấp khả năng quan sát lớn nhất) từ mỗi nút và (ii) mà không cần phải ước tính pha."} +{"idx": 1920005, "en": "We consider open quantum systems modeled as discrete lattices. Using a simple model of a single-site coupled to two leads as an example, we show that the time evolution of these systems can be analyzed in terms of an explicitly time-reversal symmetric resolution of unity. This resolution of unity includes both resonant states, which decay in the future, and anti-resonant states, which decay in the past. We show that a time-reversal invariant state contains both resonant and anti-resonant components with equal weights. However, this symmetry is automatically broken as the system evolves in time, with the resonant component becoming much larger than the anti-resonant component for t > 0 (and vice versa for t < 0). We argue that irreversibility is a manifestation of this symmetry breaking. We also compare our present approach with the subdynamics approach developed by Prof. Prigogine and collaborators. Finally, we suggest an extension of our present approach from the level of wave functions to the level of density matrices.", "gemini": "Chúng tôi xem xét các hệ thống lượng tử mở được mô hình hóa dưới dạng mạng lưới rời rạc. Sử dụng một mô hình đơn giản về một vị trí duy nhất được ghép nối với hai đầu vào làm ví dụ, chúng tôi chỉ ra rằng sự tiến hóa thời gian của các hệ thống này có thể được phân tích theo một độ phân giải hợp nhất đối xứng nghịch thời gian rõ ràng. Độ phân giải hợp nhất này bao gồm cả các trạng thái cộng hưởng, phân rã trong tương lai và các trạng thái phản cộng hưởng, phân rã trong quá khứ. Chúng tôi chỉ ra rằng một trạng thái bất biến nghịch thời gian chứa cả các thành phần cộng hưởng và phản cộng hưởng với trọng số bằng nhau. Tuy nhiên, tính đối xứng này tự động bị phá vỡ khi hệ thống tiến triển theo thời gian, với thành phần cộng hưởng trở nên lớn hơn nhiều so với thành phần phản cộng hưởng đối với t > 0 (và ngược lại đối với t < 0). Chúng tôi cho rằng tính không đảo ngược là biểu hiện của sự phá vỡ tính đối xứng này. Chúng tôi cũng so sánh cách tiếp cận hiện tại của chúng tôi với cách tiếp cận động lực phụ được Giáo sư Prigogine và cộng sự phát triển. Cuối cùng, chúng tôi đề xuất mở rộng cách tiếp cận hiện tại của chúng tôi từ cấp độ hàm sóng đến cấp độ ma trận mật độ."} +{"idx": 1920006, "en": "Yes! Very much so. A chimera state refers to the coexistence of a coherent-incoherent dynamical evolution of identically coupled oscillators. We investigate the impact of multiplexing of a layer having repulsively coupled oscillators on the occurrence of chimeras in the layer having attractively coupled identical oscillators. We report that there exists an enhancement in the appearance of the chimera state in one layer of the multiplex network in the presence of repulsive coupling in the other layer. Furthermore, we show that a small amount of inhibition or repulsive coupling in one layer is sufficient to yield the chimera state in another layer by destroying its synchronized behavior. These results can be used to obtain insight into dynamical behaviors of those systems where both attractive and repulsive couplings exist among their constituents.", "gemini": "Đúng vậy! Rất nhiều. Trạng thái chimera đề cập đến sự cùng tồn tại của quá trình tiến hóa động lực học mạch lạc-phi mạch lạc của các dao động được ghép nối giống hệt nhau. Chúng tôi nghiên cứu tác động của sự ghép kênh của một lớp có các dao động được ghép nối đẩy lùi vào sự xuất hiện của các chimera trong lớp có các dao động giống hệt nhau được ghép nối hấp dẫn. Chúng tôi báo cáo rằng có sự gia tăng trong sự xuất hiện của trạng thái chimera trong một lớp của mạng ghép kênh khi có sự ghép nối đẩy lùi trong lớp khác. Hơn nữa, chúng tôi chỉ ra rằng một lượng nhỏ ức chế hoặc ghép nối đẩy lùi trong một lớp là đủ để tạo ra trạng thái chimera trong lớp khác bằng cách phá hủy hành vi đồng bộ của nó. Những kết quả này có thể được sử dụng để hiểu sâu hơn về hành vi động lực của những hệ thống có cả sự ghép nối hấp dẫn và đẩy lùi giữa các thành phần của chúng."} +{"idx": 1920007, "en": "In Traversa and Di Ventra [Chaos 27, 023107 (2017)] we argued, without proof, that if the non-linear dynamical systems with memory describing the class of digital memcomputing machines (DMMs) have equilibrium points, then no periodic orbits can emerge. In fact, the proof of such a statement is a simple corollary of a theorem already demonstrated in Traversa and Di Ventra [Chaos 27, 023107 (2017)]. Here, we point out how to derive such a conclusion. Incidentally, the same demonstration implies absence of chaos, a result we have already demonstrated in Di Ventra and Traversa [Phys. Lett. A 381, 3255 (2017)] using topology. These results, together with those in Traversa and Di Ventra [Chaos 27, 023107 (2017)], guarantee that if the Boolean problem the DMMs are designed to solve has a solution, the system will always find it, irrespective of the initial conditions.", "gemini": "Trong Traversa và Di Ventra [Chaos 27, 023107 (2017)], chúng tôi lập luận, mà không cần bằng chứng, rằng nếu các hệ thống động phi tuyến tính có bộ nhớ mô tả lớp máy tính mem kỹ thuật số (DMM) có các điểm cân bằng, thì không có quỹ đạo tuần hoàn nào có thể xuất hiện. Trên thực tế, bằng chứng cho một tuyên bố như vậy là một hệ quả đơn giản của một định lý đã được chứng minh trong Traversa và Di Ventra [Chaos 27, 023107 (2017)]. Ở đây, chúng tôi chỉ ra cách để rút ra kết luận như vậy. Ngẫu nhiên, cùng một chứng minh ngụ ý sự vắng mặt của hỗn loạn, một kết quả mà chúng tôi đã chứng minh trong Di Ventra và Traversa [Phys. Lett. A 381, 3255 (2017)] bằng cách sử dụng tôpô. Những kết quả này, cùng với những kết quả trong Traversa và Di Ventra [Chaos 27, 023107 (2017)], đảm bảo rằng nếu bài toán Boolean mà DMM được thiết kế để giải có lời giải, hệ thống sẽ luôn tìm ra lời giải đó, bất kể điều kiện ban đầu như thế nào."} +{"idx": 1920008, "en": "Pulse-mediated interactions are common in networks of different nature. Here we develop a general framework for simulation of networks with pulse delayed coupling. We introduce the discrete map governing the dynamics of such networks and describe the computation algorithm for its numerical simulation.", "gemini": "Các tương tác được truyền qua xung là phổ biến trong các mạng có bản chất khác nhau. Ở đây, chúng tôi phát triển một khuôn khổ chung để mô phỏng các mạng với sự ghép nối trễ xung. Chúng tôi giới thiệu bản đồ rời rạc chi phối động lực học của các mạng như vậy và mô tả thuật toán tính toán để mô phỏng số của nó."} +{"idx": 1920009, "en": "In the present paper, we investigate the dynamics of a model in which the real part of the economy, described within a multiplier-accelerator framework, interacts with a financial market with heterogeneous speculators, in order to study the channels through which the two sectors influence each other. Employing analytical and numerical tools, we investigate stability conditions as well as bifurcations and possible periodic, quasi-periodic, and chaotic dynamics, enlightening how the degree of market interaction, together with the accelerator parameter and the intervention of the fiscal authority, may affect the business cycle and the course of the financial market. In particular, we show that even if the steady state is locally stable, multistability phenomena can occur, with several and complex dynamic structures coexisting with the steady state. Finally, simulations reveal that the proposed model is able to explain several statistical properties and stylized facts observed in real financial markets, including persistent high volatility, fat-tailed return distributions, volatility clustering, and positive autocorrelation of absolute returns.", "gemini": "Trong bài báo hiện tại, chúng tôi nghiên cứu động lực học của một mô hình trong đó phần thực của nền kinh tế, được mô tả trong khuôn khổ hệ số nhân-tăng tốc, tương tác với thị trường tài chính với những nhà đầu cơ không đồng nhất, để nghiên cứu các kênh mà qua đó hai lĩnh vực này ảnh hưởng lẫn nhau. Sử dụng các công cụ phân tích và số, chúng tôi nghiên cứu các điều kiện ổn định cũng như các điểm phân kỳ và động lực học hỗn loạn, tuần hoàn, bán tuần hoàn có thể xảy ra, làm sáng tỏ cách thức mà mức độ tương tác thị trường, cùng với tham số tăng tốc và sự can thiệp của cơ quan tài chính, có thể ảnh hưởng đến chu kỳ kinh doanh và diễn biến của thị trường tài chính. Đặc biệt, chúng tôi chỉ ra rằng ngay cả khi trạng thái ổn định là ổn định cục bộ, thì hiện tượng đa ổn định vẫn có thể xảy ra, với một số cấu trúc động phức tạp cùng tồn tại với trạng thái ổn định. Cuối cùng, các mô phỏng cho thấy rằng mô hình được đề xuất có khả năng giải thích một số tính chất thống kê và các sự kiện cách điệu được quan sát thấy trên thị trường tài chính thực tế, bao gồm biến động cao dai dẳng, phân phối lợi nhuận đuôi béo, cụm biến động và tự tương quan tích cực của lợi nhuận tuyệt đối."} +{"idx": 1920010, "en": "The presence of undesirable dominating signals in geophysical experimental data is a challenge in many subfields. One remarkable example is surface gravimetry, where frequencies from Earth tides correspond to time-series fluctuations up to a thousand times larger than the phenomena of major interest, such as hydrological gravity effects or co-seismic gravity changes. This work discusses general methods for the removal of unwanted dominating signals by applying them to 8 long-period gravity time-series of the International Geodynamics and Earth Tides Service, equivalent to the acquisition from 8 instruments in 5 locations representative of the network. We compare three different conceptual approaches for tide removal: frequency filtering, physical modelling, and data-based modelling. Each approach reveals a different limitation to be considered depending on the intended application. Vestiges of tides remain in the residues for the modelling procedures, whereas the signal was distorted in different ways by the filtering and data-based procedures. The linear techniques employed were power spectral density, spectrogram, cross-correlation, and classical harmonics decomposition, while the system dynamics was analysed by state-space reconstruction and estimation of the largest Lyapunov exponent. Although the tides could not be completely eliminated, they were sufficiently reduced to allow observation of geophysical events of interest above the 10 nm s-2 level, exemplified by a hydrology-related event of 60 nm s-2. The implementations adopted for each conceptual approach are general, so that their principles could be applied to other kinds of data affected by undesired signals composed mainly by periodic or quasi-periodic components.", "gemini": "Sự hiện diện của các tín hiệu thống trị không mong muốn trong dữ liệu thực nghiệm về địa vật lý là một thách thức trong nhiều lĩnh vực nhỏ. Một ví dụ đáng chú ý là trọng trắc bề mặt, trong đó các tần số từ thủy triều Trái đất tương ứng với các biến động theo chuỗi thời gian lớn hơn tới một nghìn lần so với các hiện tượng quan tâm chính, chẳng hạn như các hiệu ứng trọng lực thủy văn hoặc các thay đổi trọng lực đồng địa chấn. Công trình này thảo luận về các phương pháp chung để loại bỏ các tín hiệu thống trị không mong muốn bằng cách áp dụng chúng vào 8 chuỗi thời gian trọng lực dài hạn của Dịch vụ thủy triều Trái đất và địa động lực học quốc tế, tương đương với việc thu thập từ 8 thiết bị tại 5 địa điểm đại diện cho mạng. Chúng tôi so sánh ba cách tiếp cận khái niệm khác nhau để loại bỏ thủy triều: lọc tần số, mô hình hóa vật lý và mô hình hóa dựa trên dữ liệu. Mỗi cách tiếp cận cho thấy một hạn chế khác nhau cần được xem xét tùy thuộc vào ứng dụng dự định. Dấu vết của thủy triều vẫn còn trong các phần dư cho các quy trình mô hình hóa, trong khi tín hiệu bị bóp méo theo nhiều cách khác nhau bởi các quy trình lọc và dựa trên dữ liệu. Các kỹ thuật tuyến tính được sử dụng là mật độ phổ công suất, phổ đồ, tương quan chéo và phân tích sóng hài cổ điển, trong khi động lực học của hệ thống được phân tích bằng cách tái tạo không gian trạng thái và ước tính số mũ Lyapunov lớn nhất. Mặc dù thủy triều không thể loại bỏ hoàn toàn, nhưng chúng đã được giảm đủ để cho phép quan sát các sự kiện địa vật lý đáng quan tâm ở mức trên 10 nm s-2, ví dụ như sự kiện liên quan đến thủy văn là 60 nm s-2. Các triển khai được áp dụng cho từng cách tiếp cận khái niệm đều mang tính tổng quát, do đó các nguyên tắc của chúng có thể được áp dụng cho các loại dữ liệu khác bị ảnh hưởng bởi các tín hiệu không mong muốn chủ yếu bao gồm các thành phần tuần hoàn hoặc bán tuần hoàn."} +{"idx": 1920011, "en": "We report the first direct observation of glycolytic oscillations in HeLa cervical cancer cells, which we regard as primordial oscillations preserved in living cells. HeLa cells starved of glucose or both glucose and serum exhibited glycolytic oscillations in nicotinamide adenine dinucleotide (NADH), exhibiting asynchronous intercellular behaviors. Also found were spatially homogeneous and inhomogeneous intracellular NADH oscillations in the individual cells. Our results demonstrate that starved HeLa cells may be induced to exhibit glycolytic oscillations by either high-uptake of glucose or the enhancement of a glycolytic pathway (Crabtree effect or the Warburg effect), or both. Their asynchronous collective behaviors in the oscillations were probably due to a weak intercellular coupling. Elucidation of the relationship between the mechanism of glycolytic dynamics in cancer cells and their pathophysiological characteristics remains a challenge in future.", "gemini": "Chúng tôi báo cáo quan sát trực tiếp đầu tiên về các dao động glycolytic trong các tế bào ung thư cổ tử cung HeLa, mà chúng tôi coi là các dao động nguyên thủy được bảo tồn trong các tế bào sống. Các tế bào HeLa thiếu glucose hoặc cả glucose và huyết thanh đều biểu hiện các dao động glycolytic trong nicotinamide adenine dinucleotide (NADH), biểu hiện các hành vi tế bào không đồng bộ. Cũng tìm thấy các dao động NADH trong tế bào không đồng nhất và đồng nhất về mặt không gian trong các tế bào riêng lẻ. Kết quả của chúng tôi chứng minh rằng các tế bào HeLa bị đói có thể được kích thích để biểu hiện các dao động glycolytic bằng cách hấp thụ nhiều glucose hoặc tăng cường đường glycolytic (hiệu ứng Crabtree hoặc hiệu ứng Warburg), hoặc cả hai. Các hành vi tập thể không đồng bộ của chúng trong các dao động có thể là do sự liên kết tế bào yếu. Làm sáng tỏ mối quan hệ giữa cơ chế động lực học glycolytic trong các tế bào ung thư và các đặc điểm bệnh sinh lý của chúng vẫn là một thách thức trong tương lai."} +{"idx": 1920012, "en": "Self-organization in nonequilibrium systems resulting in the formation of dissipative structures has been studied in a variety of systems, most prominently in chemical systems. We present a study of a voltage-driven dissipative structure consisting of conducting beads immersed in a viscous medium of oil. In this simple system, we observed remarkably complex organism-like behavior. The dissipative structure consists of a tree structure that spontaneously forms and moves like a worm and exhibits many features characteristic of living organisms. The complex motion of the beads driven by the applied field, the dipole-dipole interaction between the beads, and the hydrodynamic flow of the viscous medium result in a time evolution of the tree structure towards states of lower resistance or higher dissipation and thus higher rates of entropy production. The resulting end-directed evolution manifests as the tree moving to locations seeking higher current, the current that sustains its structure and dynamics. The study of end-directed evolution in the dissipative structure gives us a means to distinguish the fundamental difference between machines and organisms and opens a path for the formulation of physics of organisms.", "gemini": "Tự tổ chức trong các hệ thống không cân bằng dẫn đến sự hình thành các cấu trúc tiêu tán đã được nghiên cứu trong nhiều hệ thống khác nhau, nổi bật nhất là trong các hệ thống hóa học. Chúng tôi trình bày một nghiên cứu về một cấu trúc tiêu tán do điện áp tạo ra bao gồm các hạt dẫn điện ngập trong môi trường dầu nhớt. Trong hệ thống đơn giản này, chúng tôi quan sát thấy hành vi giống như sinh vật phức tạp đáng kinh ngạc. Cấu trúc tiêu tán bao gồm một cấu trúc dạng cây tự phát hình thành và di chuyển giống như một con sâu và biểu hiện nhiều đặc điểm đặc trưng của sinh vật sống. Chuyển động phức tạp của các hạt do trường tác dụng, tương tác lưỡng cực-lưỡng cực giữa các hạt và dòng chảy thủy động của môi trường nhớt dẫn đến sự tiến hóa theo thời gian của cấu trúc dạng cây hướng tới các trạng thái có điện trở thấp hơn hoặc tiêu tán cao hơn và do đó có tốc độ sinh entropy cao hơn. Sự tiến hóa có hướng kết thúc biểu hiện dưới dạng cây di chuyển đến các vị trí tìm kiếm dòng điện cao hơn, dòng điện duy trì cấu trúc và động lực của nó. Nghiên cứu về sự tiến hóa có hướng kết thúc trong cấu trúc tiêu tán cung cấp cho chúng ta một phương tiện để phân biệt sự khác biệt cơ bản giữa máy móc và sinh vật và mở ra con đường cho việc xây dựng vật lý của sinh vật."} +{"idx": 1920013, "en": "The intermediate dynamics of composed one-dimensional maps is used to multiply attractors in phase space and create multiple independent bifurcation diagrams which can split apart. Results are shown for the composition of k-paradigmatic quadratic maps with distinct values of parameters generating k-independent bifurcation diagrams with corresponding k orbital points. For specific conditions, the basic mechanism for creating the shifted diagrams is the prohibition of period doubling bifurcations transformed in saddle-node bifurcations.", "gemini": "Động lực học trung gian của các bản đồ một chiều được sử dụng để nhân các bộ hấp dẫn trong không gian pha và tạo ra nhiều sơ đồ phân nhánh độc lập có thể tách rời nhau. Kết quả được hiển thị cho thành phần của các bản đồ bậc hai chuẩn k với các giá trị riêng biệt của các tham số tạo ra các sơ đồ phân nhánh độc lập k với các điểm quỹ đạo k tương ứng. Đối với các điều kiện cụ thể, cơ chế cơ bản để tạo ra các sơ đồ dịch chuyển là sự cấm các phân nhánh nhân đôi chu kỳ được chuyển đổi trong các phân nhánh nút yên."} +{"idx": 1920014, "en": "We achieved a low-cost and small-sized Rb optical frequency standard based on 85Rb 5S1/2 → 6P3/2 transition with 10-15 stability, which is comparable with that of the best 532 nm I2 optical frequency standards. In this system, we directly lock the 420 nm diode laser on the 5S1/2 F = 3 → 6P3/2 F' = 4 hyperfine transition line without an additional Pound-Drever-Hall pre-locking system. The signal-to-noise-ratio reaches as high as 350 000 when the averaging time is at 1 s. Eventually by the fluctuation of the residual error signal after locking, the preliminary stability of the optical frequency standard reaches 1.2×10-14/τ, decreasing to 2.1 × 10-15 at 80 s. It shows potential in stability performance, experimental cost, and system volume compared with the 532 nm I2 optical frequency standard as a wavelength standard. It also opens a door for the achievement of wavelength standards by using higher excited states of alkalies.", "gemini": "Chúng tôi đã đạt được một tiêu chuẩn tần số quang học Rb có chi phí thấp và kích thước nhỏ dựa trên sự chuyển đổi 85Rb 5S1/2 → 6P3/2 với độ ổn định 10-15, tương đương với độ ổn định của các tiêu chuẩn tần số quang học I2 532 nm tốt nhất. Trong hệ thống này, chúng tôi khóa trực tiếp laser diode 420 nm trên đường chuyển siêu mịn 5S1/2 F = 3 → 6P3/2 F' = 4 mà không cần hệ thống khóa trước Pound-Drever-Hall bổ sung. Tỷ số tín hiệu trên nhiễu đạt tới 350.000 khi thời gian trung bình là 1 giây. Cuối cùng, do sự biến động của tín hiệu lỗi còn lại sau khi khóa, độ ổn định sơ bộ của tiêu chuẩn tần số quang học đạt 1,2 × 10-14 / τ, giảm xuống còn 2,1 × 10-15 ở 80 giây. Nó cho thấy tiềm năng về hiệu suất ổn định, chi phí thử nghiệm và thể tích hệ thống so với tiêu chuẩn tần số quang học I2 532 nm như một tiêu chuẩn bước sóng. Nó cũng mở ra cánh cửa để đạt được các tiêu chuẩn bước sóng bằng cách sử dụng các trạng thái kích thích cao hơn của kiềm."} +{"idx": 1920015, "en": "A sputter deposition source for the use in ultrahigh vacuum (UHV) is described, and some properties of the source are analyzed. The operating principle is based on the design developed by Mayr et al. [Rev. Sci. Instrum. 84, 094103 (2013)], where electrons emitted from a filament ionize argon gas and the Ar+ ions are accelerated to the target. In contrast to the original design, two grids are used to direct a large fraction of the Ar+ ions to the target, and the source has a housing cooled by liquid nitrogen to reduce contaminations. The source has been used for the deposition of zirconium, a material that is difficult to evaporate in standard UHV evaporators. At an Ar pressure of 9×10-6 mbar in the UHV chamber and moderate emission current, a highly reproducible deposition rate of ≈1 ML in 250 s was achieved at the substrate (at a distance of ≈50 mm from the target). Higher deposition rates are easily possible. X-ray photoelectron spectroscopy shows a high purity of the deposited films. Depending on the grid voltages, the substrate gets mildly sputtered by Ar+ ions; in addition, the substrate is also reached by electrons from the negatively biased sputter target.", "gemini": "Một nguồn lắng đọng phun được mô tả để sử dụng trong chân không cực cao (UHV) và một số đặc tính của nguồn được phân tích. Nguyên lý hoạt động dựa trên thiết kế do Mayr và cộng sự phát triển. [Rev. Sci. Instrum. 84, 094103 (2013)], trong đó các electron phát ra từ sợi đốt ion hóa khí argon và các ion Ar+ được tăng tốc đến mục tiêu. Trái ngược với thiết kế ban đầu, hai lưới được sử dụng để hướng một phần lớn các ion Ar+ đến mục tiêu và nguồn có vỏ được làm mát bằng nitơ lỏng để giảm ô nhiễm. Nguồn đã được sử dụng để lắng đọng zirconium, một vật liệu khó bốc hơi trong các máy bốc hơi UHV tiêu chuẩn. Ở áp suất Ar là 9 × 10-6 mbar trong buồng UHV và dòng phát xạ vừa phải, tốc độ lắng đọng có thể tái tạo cao là ≈1 ML trong 250 s đã đạt được ở chất nền (ở khoảng cách ≈50 mm từ mục tiêu). Tốc độ lắng đọng cao hơn có thể dễ dàng thực hiện. Phổ quang điện tử tia X cho thấy độ tinh khiết cao của các lớp màng lắng đọng. Tùy thuộc vào điện áp lưới, chất nền bị các ion Ar+ bắn phá nhẹ; ngoài ra, chất nền cũng được các electron từ mục tiêu bắn phá có độ lệch âm tiếp cận."} +{"idx": 1920016, "en": "A combined experimental and modeling effort is undertaken to assess a detection system composed of an orthogonal extraction time-of-flight (TOF) mass spectrometer coupled to a continuous ion source emitting an ion beam with kinetic energy of several hundred eV. The continuous ion source comprises an electrospray capillary system employing an undiluted ionic liquid emitting directly into vacuum. The resulting ion beam consists of ions with kinetic energy distributions of width greater than a hundred of eV and mass-to-charge (m/q) ratios ranging from 111 to 500 000 amu/q. In particular, the investigation aims to demonstrate the kinetic energy resolution along the ion beam axis (axial) of orthogonally extracted ions in measurements of the axial kinetic energy-specific mass spectrum, mass flow rate, and total ion current. The described instrument is capable of simultaneous measurement of a broad m/q range in a single acquisition cycle with approximately 25 eV/q axial kinetic energy resolution. Mass resolutions of ∼340 (M/ΔM, FWHM) were obtained for ions at m/q = 1974. Comparison of the orthogonally extracted TOF mass spectrum to mass flow and ion current measurements obtained with a quartz-crystal microbalance and Faraday cup, respectively, shows reasonable numeric agreement and qualitative agreement in the trend as a function of energy defect.", "gemini": "Một nỗ lực kết hợp giữa thực nghiệm và mô hình hóa được thực hiện để đánh giá một hệ thống phát hiện bao gồm máy quang phổ khối thời gian bay (TOF) chiết xuất trực giao kết hợp với nguồn ion liên tục phát ra chùm ion có động năng của vài trăm eV. Nguồn ion liên tục bao gồm một hệ thống mao dẫn điện phun sử dụng chất lỏng ion không pha loãng phát trực tiếp vào chân không. Chùm ion thu được bao gồm các ion có phân bố động năng có độ rộng lớn hơn hàng trăm eV và tỷ số khối lượng trên điện tích (m/q) trong khoảng từ 111 đến 500.000 amu/q. Đặc biệt, mục đích của cuộc điều tra là chứng minh độ phân giải động năng dọc theo trục chùm ion (trục) của các ion được chiết xuất trực giao trong các phép đo phổ khối lượng động năng trục cụ thể, tốc độ dòng khối lượng và tổng cường độ ion. Thiết bị được mô tả có khả năng đo đồng thời một phạm vi m/q rộng trong một chu kỳ thu nhận duy nhất với độ phân giải động năng trục khoảng 25 eV/q. Độ phân giải khối lượng ∼340 (M/ΔM, FWHM) đã thu được đối với các ion ở m/q = 1974. So sánh phổ khối lượng TOF được chiết xuất trực giao với các phép đo dòng khối lượng và cường độ ion thu được bằng cân tinh thể thạch anh và cốc Faraday tương ứng, cho thấy sự đồng ý về số và sự đồng ý về chất lượng trong xu hướng như một hàm của khuyết tật năng lượng."} +{"idx": 1920017, "en": "Complex natural and engineered systems are ubiquitous, and their behavior is challenging to characterize and control. We examine the design of the entrainment process for an uncountably infinite collection of coupled phase oscillators that are all subject to the same periodic driving signal. In the absence of coupling, an appropriately designed input can result in each oscillator attaining the frequency of the driving signal, with a phase offset determined by its natural frequency. We consider a special case of interacting oscillators in which the coupling tends to destabilize the phase configuration to which the driving signal would send the collection in the absence of coupling. In this setting, we derive stability results that characterize the trade-off between the effects of driving and coupling, and compare these results to the well-known Kuramoto model of a collection of free-running coupled oscillators.", "gemini": "Các hệ thống tự nhiên và kỹ thuật phức tạp có ở khắp mọi nơi và hành vi của chúng rất khó xác định và kiểm soát. Chúng tôi kiểm tra thiết kế của quá trình cuốn theo cho một tập hợp vô hạn các dao động pha ghép nối không đếm được, tất cả đều chịu cùng một tín hiệu điều khiển tuần hoàn. Khi không có sự ghép nối, một đầu vào được thiết kế phù hợp có thể khiến mỗi dao động đạt được tần số của tín hiệu điều khiển, với độ lệch pha được xác định bởi tần số tự nhiên của nó. Chúng tôi xem xét một trường hợp đặc biệt của các dao động tương tác trong đó sự ghép nối có xu hướng làm mất ổn định cấu hình pha mà tín hiệu điều khiển sẽ gửi đến tập hợp khi không có sự ghép nối. Trong bối cảnh này, chúng tôi thu được các kết quả ổn định đặc trưng cho sự cân bằng giữa các hiệu ứng của điều khiển và ghép nối, đồng thời so sánh các kết quả này với mô hình Kuramoto nổi tiếng về một tập hợp các dao động ghép nối chạy tự do."} +{"idx": 1920018, "en": "We have built a variable temperature scanning probe microscope (SPM) that covers 4.6 K-180 K and up to 7 T whose SPM head fits in a 52 mm bore magnet. It features a temperature-controlled sample stage thermally well isolated from the SPM body in good thermal contact with the liquid helium bath. It has a 7-sample-holder storage carousel at liquid helium temperature for systematic studies using multiple samples and field emission targets intended for spin-polarized spectroscopic-imaging scanning tunneling microscopy (STM) study on samples with various compositions and doping conditions. The system is equipped with a UHV sample preparation chamber and mounted on a two-stage vibration isolation system made of a heavy concrete block and a granite table on pneumatic vibration isolators. A quartz resonator (qPlus)-based non-contact atomic force microscope (AFM) sensor is used for simultaneous STM/AFM operation for research on samples with highly insulating properties such as strongly underdoped cuprates and strongly correlated electron systems.", "gemini": "Chúng tôi đã chế tạo một kính hiển vi quét đầu dò nhiệt độ biến đổi (SPM) bao phủ 4,6 K-180 K và lên đến 7 T, đầu SPM của nó vừa với nam châm có đường kính 52 mm. Nó có một giai đoạn mẫu được kiểm soát nhiệt độ được cách nhiệt tốt về mặt nhiệt khỏi thân SPM tiếp xúc nhiệt tốt với bồn tắm heli lỏng. Nó có băng chuyền lưu trữ 7 giá đỡ mẫu ở nhiệt độ heli lỏng để nghiên cứu có hệ thống bằng cách sử dụng nhiều mẫu và mục tiêu phát xạ trường nhằm vào nghiên cứu quét đường hầm kính hiển vi quang phổ phân cực spin (STM) trên các mẫu có nhiều thành phần và điều kiện pha tạp khác nhau. Hệ thống được trang bị buồng chuẩn bị mẫu UHV và được gắn trên hệ thống cách ly rung động hai giai đoạn được làm bằng khối bê tông nặng và bàn đá granit trên bộ cách ly rung động khí nén. Một cảm biến kính hiển vi lực nguyên tử (AFM) không tiếp xúc dựa trên cộng hưởng thạch anh (qPlus) được sử dụng để vận hành STM/AFM đồng thời để nghiên cứu các mẫu có tính chất cách điện cao như đồng oxit dưới pha tạp mạnh và hệ thống electron tương quan mạnh."} +{"idx": 1920019, "en": "We describe the design, fabrication, and validation of a cryogenically compatible quasioptical thermal source for characterization of detector arrays. The source is constructed using a graphite-loaded epoxy mixture that is molded into a tiled pyramidal structure. The mold is fabricated using a hardened steel template produced via a wire electron discharge machining process. The absorptive mixture is bonded to a copper backplate enabling thermalization of the entire structure and measurement of the source temperature. Measurements indicate that the reflectance of the source is <0.001 across a spectral band extending from 75 to 330 GHz.", "gemini": "Chúng tôi mô tả thiết kế, chế tạo và xác nhận nguồn nhiệt quang học tương thích với nhiệt độ cực thấp để đặc trưng mảng đầu dò. Nguồn được chế tạo bằng cách sử dụng hỗn hợp epoxy chứa than chì được đúc thành cấu trúc hình chóp xếp lớp. Khuôn được chế tạo bằng cách sử dụng khuôn thép cứng được sản xuất thông qua quy trình gia công bằng tia điện tử dây. Hỗn hợp hấp thụ được liên kết với tấm nền bằng đồng cho phép nhiệt hóa toàn bộ cấu trúc và đo nhiệt độ nguồn. Các phép đo cho thấy độ phản xạ của nguồn là <0,001 trên băng tần quang phổ trải dài từ 75 đến 330 GHz."} +{"idx": 1920020, "en": "The complexity of cardiac fibrillation dynamics can be assessed by analyzing the distribution of phase singularities (PSs) observed using mapping systems. Interelectrode distance, however, limits the accuracy of PS detection. To investigate in a theoretical framework the PS false negative and false positive rates in relation to the characteristics of the mapping system and fibrillation dynamics, we propose a statistical model of phase maps with controllable number and locations of PSs. In this model, phase maps are generated from randomly distributed PSs with physiologically-plausible directions of rotation. Noise and distortion of the phase are added. PSs are detected using topological charge contour integrals on regular grids of varying resolutions. Over 100 × 106 realizations of the random field process are used to estimate average false negative and false positive rates using a Monte-Carlo approach. The false detection rates are shown to depend on the average distance between neighboring PSs expressed in units of interelectrode distance, following approximately a power law with exponents in the range of 1.14 to 2 for false negatives and around 2.8 for false positives. In the presence of noise or distortion of phase, false detection rates at high resolution tend to a non-zero noise-dependent lower bound. This model provides an easy-to-implement tool for benchmarking PS detection algorithms over a broad range of configurations with multiple PSs.", "gemini": "Có thể đánh giá độ phức tạp của động học rung tâm nhĩ bằng cách phân tích sự phân bố các điểm kỳ dị pha (PS) quan sát được bằng cách sử dụng các hệ thống lập bản đồ. Tuy nhiên, khoảng cách giữa các điện cực hạn chế độ chính xác của việc phát hiện PS. Để điều tra trong một khuôn khổ lý thuyết về tỷ lệ âm tính giả và dương tính giả của PS liên quan đến các đặc điểm của hệ thống lập bản đồ và động học rung tâm nhĩ, chúng tôi đề xuất một mô hình thống kê về các bản đồ pha với số lượng và vị trí của PS có thể kiểm soát được. Trong mô hình này, các bản đồ pha được tạo ra từ các PS phân bố ngẫu nhiên với các hướng quay hợp lý về mặt sinh lý. Tiếng ồn và độ méo của pha được thêm vào. PS được phát hiện bằng cách sử dụng các tích phân đường viền điện tích tôpô trên các lưới đều có độ phân giải khác nhau. Hơn 100 × 106 phép thực hiện của quá trình trường ngẫu nhiên được sử dụng để ước tính tỷ lệ âm tính giả và dương tính giả trung bình bằng cách sử dụng phương pháp Monte-Carlo. Tỷ lệ phát hiện sai được chứng minh là phụ thuộc vào khoảng cách trung bình giữa các PS lân cận được biểu thị bằng đơn vị khoảng cách giữa các điện cực, gần đúng theo định luật lũy thừa với số mũ trong phạm vi từ 1,14 đến 2 đối với âm tính giả và khoảng 2,8 đối với dương tính giả. Khi có tiếng ồn hoặc độ méo pha, tỷ lệ phát hiện sai ở độ phân giải cao có xu hướng đến một giới hạn dưới không bằng 0 phụ thuộc vào tiếng ồn. Mô hình này cung cấp một công cụ dễ triển khai để đánh giá chuẩn các thuật toán phát hiện PS trên nhiều cấu hình khác nhau với nhiều PS."} +{"idx": 1920021, "en": "A modified optics based technique was developed to effectively suppress spurious background signals encountered in the photoreflectance (PR) spectra obtained from bulk semiconductors and semiconductor microstructures. Based on a traditional PR setup, the novel PR approach utilized an achromatic beam reduction system to narrow the profile of a probe beam, which was subsequently collected by a focus lens coupled with an iris. The new setup guarantees high collection efficiency for probe signals and strong suppression of background signals. Compared with existing PR methods for background minimization, the proposed PR technique is simpler to accomplish, more cost effective, and provides a higher signal-to-noise ratio for the PR spectra.", "gemini": "Một kỹ thuật dựa trên quang học đã được phát triển để ức chế hiệu quả các tín hiệu nền giả tạo gặp phải trong quang phổ phản xạ quang (PR) thu được từ chất bán dẫn khối và vi cấu trúc bán dẫn. Dựa trên thiết lập PR truyền thống, phương pháp PR mới sử dụng hệ thống giảm chùm tia không sắc để thu hẹp cấu hình của chùm tia thăm dò, sau đó được thu thập bởi thấu kính hội tụ kết hợp với mống mắt. Thiết lập mới đảm bảo hiệu suất thu thập cao đối với các tín hiệu thăm dò và khả năng ức chế mạnh các tín hiệu nền. So với các phương pháp PR hiện có để giảm thiểu nền, kỹ thuật PR được đề xuất đơn giản hơn để thực hiện, hiệu quả hơn về chi phí và cung cấp tỷ lệ tín hiệu trên nhiễu cao hơn cho quang phổ PR."} +{"idx": 1920022, "en": "Thermoacoustic instability, caused by a positive feedback between the unsteady heat release and the acoustic field in a combustor, is a major challenge faced in most practical combustors such as those used in rockets and gas turbines. We employ the synchronization theory for understanding the coupling between the unsteady heat release and the acoustic field of a thermoacoustic system. Interactions between coupled subsystems exhibiting different collective dynamics such as periodic, quasiperiodic, and chaotic oscillations are addressed. Even though synchronization studies have focused on different dynamical states separately, synchronous behaviour of two coupled systems exhibiting a quasiperiodic route to chaos has not been studied. In this study, we report the first experimental observation of different synchronous behaviours between two subsystems of a thermoacoustic system exhibiting such a transition as reported in Kabiraj et al. [Chaos 22, 023129 (2012)]. A rich variety of synchronous behaviours such as phase locking, intermittent phase locking, and phase drifting are observed as the dynamics of such subsystem change. The observed synchronization behaviour is further characterized using phase locking value, correlation coefficient, and relative mean frequency. These measures clearly reveal the boundaries between different states of synchronization.", "gemini": "Sự bất ổn nhiệt âm, do phản hồi tích cực giữa quá trình giải phóng nhiệt không ổn định và trường âm trong buồng đốt, là một thách thức lớn đối mặt với hầu hết các buồng đốt thực tế như những buồng được sử dụng trong tên lửa và tua bin khí. Chúng tôi sử dụng lý thuyết đồng bộ hóa để hiểu sự liên kết giữa quá trình giải phóng nhiệt không ổn định và trường âm của hệ thống nhiệt âm. Các tương tác giữa các hệ thống con ghép nối thể hiện các động lực tập thể khác nhau như dao động tuần hoàn, bán tuần hoàn và hỗn loạn được giải quyết. Mặc dù các nghiên cứu về đồng bộ hóa tập trung vào các trạng thái động lực học khác nhau một cách riêng biệt, nhưng hành vi đồng bộ của hai hệ thống ghép nối thể hiện một con đường bán tuần hoàn dẫn đến hỗn loạn vẫn chưa được nghiên cứu. Trong nghiên cứu này, chúng tôi báo cáo quan sát thực nghiệm đầu tiên về các hành vi đồng bộ khác nhau giữa hai hệ thống con của một hệ thống nhiệt âm học thể hiện sự chuyển đổi như được báo cáo trong Kabiraj et al. [Chaos 22, 023129 (2012)]. Một loạt các hành vi đồng bộ phong phú như khóa pha, khóa pha không liên tục và trôi pha được quan sát thấy khi động lực của hệ thống con đó thay đổi. Hành vi đồng bộ được quan sát thấy được đặc trưng thêm bằng cách sử dụng giá trị khóa pha, hệ số tương quan và tần số trung bình tương đối. Các biện pháp này cho thấy rõ ràng ranh giới giữa các trạng thái đồng bộ khác nhau."} +{"idx": 1920023, "en": "In this work, we consider a ring of coupled electronic (Wien-bridge) oscillators from a perspective combining modeling, simulation, and experimental observation. Following up on earlier work characterizing the pairwise interaction of Wien-bridge oscillators by Kuramoto-Sakaguchi phase dynamics, we develop a lattice model for a chain thereof, featuring an exponentially decaying spatial kernel. We find that for certain values of the Sakaguchi parameter α, states of traveling phase-domain fronts involving the coexistence of two clearly separated regions of distinct dynamical behavior, can establish themselves in the ring lattice. Experiments and simulations show that stationary coexistence domains of synchronization only manifest themselves with the introduction of a local impurity; here an incoherent cluster of oscillators can arise reminiscent of the chimera states in a range of systems with homogeneous oscillators and suitable nonlocal interactions between them.", "gemini": "Trong công trình này, chúng tôi xem xét một vòng các dao động điện tử ghép nối (cầu Wien) từ góc nhìn kết hợp mô hình hóa, mô phỏng và quan sát thực nghiệm. Tiếp theo công trình trước đó mô tả tương tác từng cặp của các dao động cầu Wien theo động lực pha Kuramoto-Sakaguchi, chúng tôi phát triển một mô hình mạng cho một chuỗi trong số đó, có đặc điểm là hạt nhân không gian phân rã theo cấp số nhân. Chúng tôi thấy rằng đối với một số giá trị nhất định của tham số Sakaguchi α, các trạng thái của mặt trận miền pha di chuyển liên quan đến sự cùng tồn tại của hai vùng riêng biệt rõ ràng về hành vi động lực học khác biệt, có thể tự thiết lập trong mạng lưới vòng. Các thí nghiệm và mô phỏng cho thấy rằng các miền cùng tồn tại ổn định của sự đồng bộ hóa chỉ biểu hiện rõ ràng khi có sự xuất hiện của một tạp chất cục bộ; ở đây một cụm dao động không mạch lạc có thể phát sinh gợi nhớ đến các trạng thái chimera trong một loạt các hệ thống có các dao động đồng nhất và các tương tác phi cục bộ phù hợp giữa chúng."} +{"idx": 1920024, "en": "This work reports on the complete design and the properties of an ionization gauge based on a carbon nanotube cathode, which can measure ultrahigh vacuum without thermal effects. The gauge is composed of a pressure sensor and an electronic controller. This pressure sensor is constructed based on a hot-cathode ionization gauge, where the traditional hot filament is replaced by an electron source prepared with multi-wall nanotubes. Besides, an electronic controller was developed for bias voltage supply, low current detection, and pressure indication. The gauge was calibrated in the pressure range of 10-8 to 10-4 Pa in a XHV/UHV calibration apparatus. The gauge shows good linear characteristics in different gases. The calibrated sensitivity is 0.035 Pa-1 in N2, and the standard deviation of the sensitivity is about 1.1%. In addition, the stability of the sensitivity was learned in a long period. The standard deviation of the sensitivity factor \"S\" during one year is 2.0% for Ar and 1.6% for N2.", "gemini": "Nghiên cứu này báo cáo về thiết kế hoàn chỉnh và các tính chất của đồng hồ đo ion hóa dựa trên catốt ống nano cacbon, có thể đo chân không cực cao mà không có hiệu ứng nhiệt. Đồng hồ đo bao gồm cảm biến áp suất và bộ điều khiển điện tử. Cảm biến áp suất này được chế tạo dựa trên đồng hồ đo ion hóa catốt nóng, trong đó sợi đốt nóng truyền thống được thay thế bằng nguồn electron được chế tạo bằng ống nano nhiều vách. Bên cạnh đó, một bộ điều khiển điện tử đã được phát triển để cấp điện áp phân cực, phát hiện dòng điện thấp và chỉ báo áp suất. Đồng hồ đo đã được hiệu chuẩn trong phạm vi áp suất từ 10-8 đến 10-4 Pa trong thiết bị hiệu chuẩn XHV/UHV. Đồng hồ đo cho thấy đặc tính tuyến tính tốt ở các loại khí khác nhau. Độ nhạy được hiệu chuẩn là 0,035 Pa-1 trong N2 và độ lệch chuẩn của độ nhạy là khoảng 1,1%. Ngoài ra, độ ổn định của độ nhạy đã được tìm hiểu trong một thời gian dài. Độ lệch chuẩn của hệ số độ nhạy \"S\" trong một năm là 2,0% đối với Ar và 1,6% đối với N2."} +{"idx": 1920025, "en": "It is interesting and of significant importance to investigate how network structures co-evolve with opinions. In this article, we show that, a simple model integrating consensus formation, link rewiring, and opinion change allows complex system dynamics to emerge, driving the system into a dynamic equilibrium with the co-existence of diversified opinions. Specifically, similar opinion holders may form into communities yet with no strict community consensus; and rather than being separated into disconnected communities, different communities are connected by a non-trivial proportion of inter-community links. More importantly, we show that the complex dynamics may lead to different numbers of communities at the steady state with a given tolerance between different opinion holders. We construct a framework for theoretically analyzing the co-evolution process. Theoretical analysis and extensive simulation results reveal some useful insights into the complex co-evolution process, including the formation of dynamic equilibrium, the transition between different steady states with different numbers of communities, and the dynamics between opinion distribution and network modularity.", "gemini": "Việc nghiên cứu cách các cấu trúc mạng lưới tiến hóa đồng thời với các ý kiến là điều thú vị và có ý nghĩa quan trọng. Trong bài viết này, chúng tôi chỉ ra rằng, một mô hình đơn giản tích hợp sự hình thành sự đồng thuận, sự sắp xếp lại liên kết và sự thay đổi ý kiến cho phép động lực hệ thống phức tạp xuất hiện, đưa hệ thống vào trạng thái cân bằng động với sự cùng tồn tại của các ý kiến đa dạng. Cụ thể, những người có quan điểm tương tự có thể hình thành thành các cộng đồng nhưng không có sự đồng thuận nghiêm ngặt của cộng đồng; và thay vì bị tách thành các cộng đồng không liên kết, các cộng đồng khác nhau được kết nối bởi một tỷ lệ không tầm thường các liên kết giữa các cộng đồng. Quan trọng hơn, chúng tôi chỉ ra rằng động lực phức tạp có thể dẫn đến số lượng cộng đồng khác nhau ở trạng thái ổn định với một ngưỡng chịu đựng nhất định giữa những người có quan điểm khác nhau. Chúng tôi xây dựng một khuôn khổ để phân tích lý thuyết quá trình tiến hóa đồng thời. Phân tích lý thuyết và kết quả mô phỏng mở rộng cho thấy một số hiểu biết hữu ích về quá trình tiến hóa đồng thời phức tạp, bao gồm sự hình thành trạng thái cân bằng động, sự chuyển đổi giữa các trạng thái ổn định khác nhau với số lượng cộng đồng khác nhau và động lực giữa phân phối ý kiến và tính mô đun của mạng."} +{"idx": 1920026, "en": "The frequency-dependent complex impedance of an atomic force microscope photodetector is measured. The inverse problem is solved obtaining the voltage that would have been collected with a hypothetical, perfectly flat-frequency-response photodetector from the experimentally available voltage. This information is used to study the distortion that the true input signal undergoes as it passes through the photodetector on the way to becoming the experimentally measured output signal. It is found that signals with features of interest shorter than 10 μs render noticeable differences between the true and measured raw voltages and forces. Signals with features shorter than 1 μs produce experimentally measured force curves that deviate substantially from the true force curves. A method is proposed for correcting the measured raw voltage signal.", "gemini": "Trở kháng phức hợp phụ thuộc tần số của một máy đo ảnh lực nguyên tử được đo. Bài toán ngược được giải bằng cách thu được điện áp đã được thu thập bằng một máy đo ảnh lực phản hồi tần số phẳng hoàn hảo theo giả thuyết từ điện áp có sẵn trong thực nghiệm. Thông tin này được sử dụng để nghiên cứu sự biến dạng mà tín hiệu đầu vào thực sự trải qua khi nó đi qua máy đo ảnh lực trên đường trở thành tín hiệu đầu ra được đo bằng thực nghiệm. Người ta thấy rằng các tín hiệu có các đặc điểm quan tâm ngắn hơn 10 μs tạo ra sự khác biệt đáng chú ý giữa điện áp thô thực và đo được và lực. Các tín hiệu có các đặc điểm ngắn hơn 1 μs tạo ra các đường cong lực được đo bằng thực nghiệm chênh lệch đáng kể so với các đường cong lực thực. Một phương pháp được đề xuất để hiệu chỉnh tín hiệu điện áp thô được đo."} +{"idx": 1920027, "en": "A novel algorithm for the ultrashort laser pulse characterization method of interferometric frequency-resolved optical gating (iFROG) is presented. Based on a genetic method, namely, differential evolution, the algorithm can exploit all available information of an iFROG measurement to retrieve the complex electric field of a pulse. The retrieval is subjected to a series of numerical tests to prove the robustness of the algorithm against experimental artifacts and noise. These tests show that the integrated error-correction mechanisms of the iFROG method can be successfully used to remove the effect from timing errors and spectrally varying efficiency in the detection. Moreover, the accuracy and noise resilience of the new algorithm are shown to outperform retrieval based on the generalized projections algorithm, which is widely used as the standard method in FROG retrieval. The differential evolution algorithm is further validated with experimental data, measured with unamplified three-cycle pulses from a mode-locked Ti:sapphire laser. Additionally introducing group delay dispersion in the beam path, the retrieval results show excellent agreement with independent measurements with a commercial pulse measurement device based on spectral phase interferometry for direct electric-field retrieval. Further experimental tests with strongly attenuated pulses indicate resilience of differential-evolution-based retrieval against massive measurement noise.", "gemini": "Một thuật toán mới cho phương pháp đặc tính xung laser cực ngắn của cổng quang học giải tần số giao thoa (iFROG) được trình bày. Dựa trên phương pháp di truyền, cụ thể là tiến hóa vi sai, thuật toán có thể khai thác tất cả thông tin có sẵn của phép đo iFROG để thu hồi trường điện phức hợp của xung. Việc thu hồi phải trải qua một loạt các thử nghiệm số để chứng minh tính mạnh mẽ của thuật toán đối với các hiện vật và nhiễu thực nghiệm. Các thử nghiệm này cho thấy các cơ chế hiệu chỉnh lỗi tích hợp của phương pháp iFROG có thể được sử dụng thành công để loại bỏ hiệu ứng từ lỗi thời gian và hiệu suất thay đổi theo phổ trong quá trình phát hiện. Hơn nữa, độ chính xác và khả năng chống nhiễu của thuật toán mới được chứng minh là vượt trội so với việc thu hồi dựa trên thuật toán chiếu tổng quát, được sử dụng rộng rãi như phương pháp chuẩn trong việc thu hồi FROG. Thuật toán tiến hóa vi sai được xác thực thêm bằng dữ liệu thực nghiệm, được đo bằng các xung ba chu kỳ không được khuếch đại từ laser Ti: sapphire khóa chế độ. Ngoài ra, đưa thêm độ phân tán độ trễ nhóm vào đường truyền chùm tia, kết quả thu hồi cho thấy sự đồng ý tuyệt vời với các phép đo độc lập bằng thiết bị đo xung thương mại dựa trên giao thoa pha phổ để thu hồi trường điện trực tiếp. Các thử nghiệm thực nghiệm khác với các xung bị suy giảm mạnh cho thấy khả năng chống chịu của việc thu hồi dựa trên tiến hóa vi sai đối với nhiễu đo lớn."} +{"idx": 1920028, "en": "A low-cost system able to perform microtomography of samples such as teeth, insects, or other small materials and low atomic numbers is presented. For this, a small flat panel type sensor was used. The process of characterization of the detector is detailed, as well as its main characteristics. The electromechanical control and the software used are also described. The advantages, some limitations, and comparisons with commercial systems are presented along with some three-dimensional volumetric reconstruction of different materials that served as samples during the development of the system.", "gemini": "Một hệ thống giá rẻ có khả năng chụp cắt lớp vi tính các mẫu vật như răng, côn trùng hoặc các vật liệu nhỏ khác và số nguyên tử thấp được trình bày. Đối với điều này, một cảm biến loại bảng phẳng nhỏ đã được sử dụng. Quá trình đặc tính của máy dò được trình bày chi tiết, cũng như các đặc điểm chính của nó. Hệ thống điều khiển cơ điện và phần mềm được sử dụng cũng được mô tả. Các ưu điểm, một số hạn chế và so sánh với các hệ thống thương mại được trình bày cùng với một số tái tạo thể tích ba chiều của các vật liệu khác nhau đóng vai trò là mẫu trong quá trình phát triển hệ thống."} +{"idx": 1920029, "en": "It is common that a physical system resonates at a particular frequency, whose frequency depends on physical parameters which may change in time. Often, one would like to automatically track this signal as the frequency changes, measuring, for example, its amplitude. In scientific research, one would also like to utilize the standard methods, such as lock-in amplifiers, to improve the signal to noise ratio. We present a complete He ii second sound system that uses positive feedback to generate a sinusoidal signal of constant amplitude via automatic gain control. This signal is used to produce temperature/entropy waves (second sound) in superfluid helium-4 (He ii). A lock-in amplifier limits the oscillation to a desirable frequency and demodulates the received sound signal. Using this tracking system, a second sound signal probed turbulent decay in He ii. We present results showing that the tracking system is more reliable than those of a conventional fixed frequency method; there is less correlation with temperature (frequency) fluctuation when the tracking system is used.", "gemini": "Thông thường, một hệ thống vật lý cộng hưởng ở một tần số cụ thể, tần số này phụ thuộc vào các thông số vật lý có thể thay đổi theo thời gian. Thông thường, người ta muốn tự động theo dõi tín hiệu này khi tần số thay đổi, chẳng hạn như đo biên độ của nó. Trong nghiên cứu khoa học, người ta cũng muốn sử dụng các phương pháp tiêu chuẩn, chẳng hạn như bộ khuếch đại đồng bộ, để cải thiện tỷ lệ tín hiệu trên nhiễu. Chúng tôi trình bày một hệ thống âm thanh thứ hai hoàn chỉnh của He ii sử dụng phản hồi tích cực để tạo ra tín hiệu hình sin có biên độ không đổi thông qua điều khiển khuếch đại tự động. Tín hiệu này được sử dụng để tạo ra sóng nhiệt độ/entropy (âm thanh thứ hai) trong heli-4 siêu lỏng (He ii). Bộ khuếch đại đồng bộ giới hạn dao động ở tần số mong muốn và giải điều chế tín hiệu âm thanh thu được. Sử dụng hệ thống theo dõi này, một tín hiệu âm thanh thứ hai đã thăm dò sự phân rã nhiễu động trong He ii. Chúng tôi trình bày các kết quả cho thấy hệ thống theo dõi đáng tin cậy hơn so với các phương pháp tần số cố định thông thường; có ít tương quan hơn với biến động nhiệt độ (tần số) khi sử dụng hệ thống theo dõi."} +{"idx": 1920030, "en": "Permanent magnet spherical actuator (PMSA) is a multi-variable featured and inter-axis coupled nonlinear system, which unavoidably compromises its motion control implementation. Uncertainties such as external load and friction torque of ball bearing and manufacturing errors also influence motion performance significantly. Therefore, the objective of this paper is to propose a controller based on a single neural adaptive (SNA) algorithm and a neural network (NN) identifier optimized with a particle swarm optimization (PSO) algorithm to improve the motion stability of PMSA with three-dimensional magnet arrays. The dynamic model and computed torque model are formulated for the spherical actuator, and a dynamic decoupling control algorithm is developed. By utilizing the global-optimization property of the PSO algorithm, the NN identifier is trained to avoid locally optimal solution and achieve high-precision compensations to uncertainties. The employment of the SNA controller helps to reduce the effect of compensation errors and convert the system to a stable one, even if there is difference between the compensations and uncertainties due to external disturbances. A simulation model is established, and experiments are conducted on the research prototype to validate the proposed control algorithm. The amplitude of the parameter perturbation is set to 5%, 10%, and 15%, respectively. The strong robustness of the proposed hybrid algorithm is validated by the abundant simulation data. It shows that the proposed algorithm can effectively compensate the influence of uncertainties and eliminate the effect of inter-axis couplings of the spherical actuator.", "gemini": "Bộ truyền động hình cầu nam châm vĩnh cửu (PMSA) là hệ thống phi tuyến đa biến và liên trục, điều này không thể tránh khỏi sẽ ảnh hưởng đến quá trình thực hiện điều khiển chuyển động của nó. Các yếu tố không chắc chắn như tải trọng bên ngoài và mômen ma sát của ổ bi và lỗi sản xuất cũng ảnh hưởng đáng kể đến hiệu suất chuyển động. Do đó, mục tiêu của bài báo này là đề xuất một bộ điều khiển dựa trên thuật toán thích ứng thần kinh đơn (SNA) và một nhận dạng mạng nơ-ron (NN) được tối ưu hóa bằng thuật toán tối ưu hóa đàn hạt (PSO) để cải thiện độ ổn định chuyển động của PMSA với các mảng nam châm ba chiều. Mô hình động và mô hình mô-men tính toán được xây dựng cho bộ truyền động hình cầu và một thuật toán điều khiển tách rời động được phát triển. Bằng cách sử dụng thuộc tính tối ưu hóa toàn cục của thuật toán PSO, nhận dạng NN được đào tạo để tránh giải pháp tối ưu cục bộ và đạt được khả năng bù đắp chính xác cao cho các yếu tố không chắc chắn. Việc sử dụng bộ điều khiển SNA giúp giảm tác động của các lỗi bù và chuyển hệ thống thành hệ thống ổn định, ngay cả khi có sự khác biệt giữa các phép bù và các yếu tố không chắc chắn do nhiễu bên ngoài. Một mô hình mô phỏng được thiết lập và các thí nghiệm được tiến hành trên nguyên mẫu nghiên cứu để xác thực thuật toán điều khiển được đề xuất. Biên độ nhiễu tham số được đặt thành 5%, 10% và 15%. Tính mạnh mẽ của thuật toán lai được đề xuất được xác thực bởi dữ liệu mô phỏng phong phú. Điều đó cho thấy thuật toán được đề xuất có thể bù đắp hiệu quả ảnh hưởng của các yếu tố không chắc chắn và loại bỏ tác động của các liên kết liên trục của bộ truyền động hình cầu."} +{"idx": 1920031, "en": "We present the design and construction of an NMR probe to investigate single crystals under strain at cryogenic temperatures. The probe head incorporates a piezoelectric-based apparatus from Razorbill Instruments that enables both compressive and tensile strain tuning up to strain values on the order of 0.3% with a precision of 0.001%.   75As NMR in BaFe2As2 reveals large changes to the electric field gradient and indicates that the strain is homogeneous to within 16% over the volume of the NMR coil.", "gemini": "Chúng tôi trình bày thiết kế và cấu tạo của đầu dò NMR để nghiên cứu các tinh thể đơn dưới ứng suất ở nhiệt độ cực thấp. Đầu dò kết hợp một thiết bị dựa trên áp điện từ Razorbill Instruments cho phép điều chỉnh ứng suất nén và kéo lên đến các giá trị ứng suất vào khoảng 0,3% với độ chính xác 0,001%.   75As NMR trong BaFe2As2 cho thấy những thay đổi lớn đối với độ dốc trường điện và chỉ ra rằng ứng suất đồng nhất trong vòng 16% trên thể tích của cuộn dây NMR."} +{"idx": 1920032, "en": "In order to avoid a fringe jump caused by high plasma density and pellet injection [Y. Zhou et al., Rev. Sci. Instrum. 87, 11E107 (2016)], a new CO2 dispersion interferometer is designed and commissioned on HL-2A for average line-density measurement and density feedback control. The second harmonic technology in this system eliminates the phase shift caused by mechanical vibration. Signals are processed by a digital phase comparator and can be monitored in real time. A series of experiments are conducted to study the characteristics of the system such as a second harmonic coefficient and long-term stability. The resolution of density measurement is less than 8 × 1017/m3, and the experiment result on HL-2A demonstrates the interferometer's capability to track plasma density evolution with rapid change. The system also shows good stability against mechanical vibrations.", "gemini": "Để tránh sự nhảy nhiễu gây ra bởi mật độ plasma cao và sự phun viên [Y. Zhou et al., Rev. Sci. Instrum. 87, 11E107 (2016)], một giao thoa kế phân tán CO2 mới được thiết kế và đưa vào hoạt động trên HL-2A để đo mật độ đường trung bình và kiểm soát phản hồi mật độ. Công nghệ sóng hài thứ hai trong hệ thống này loại bỏ sự dịch pha do rung động cơ học. Các tín hiệu được xử lý bởi bộ so sánh pha kỹ thuật số và có thể được theo dõi theo thời gian thực. Một loạt các thí nghiệm được tiến hành để nghiên cứu các đặc tính của hệ thống như hệ số sóng hài thứ hai và độ ổn định lâu dài. Độ phân giải của phép đo mật độ nhỏ hơn 8 × 1017/m3 và kết quả thí nghiệm trên HL-2A chứng minh khả năng theo dõi sự tiến hóa mật độ plasma với sự thay đổi nhanh chóng của giao thoa kế. Hệ thống cũng cho thấy độ ổn định tốt chống lại các rung động cơ học."} +{"idx": 1920033, "en": "This paper describes the design and construction of a compact, \"user-friendly\" polarization-modulation infrared reflection absorption spectroscopy (PM-IRRAS) instrument at the Center for Functional Nanomaterials (CFN) of Brookhaven National Laboratory, which allows studying surfaces at pressures ranging from ultra-high vacuum to 100 Torr. Surface infrared spectroscopy is ideally suited for studying these processes as the vibrational frequencies of the IR chromophores are sensitive to the nature of the bonding environment on the surface. Relying on the surface selection rules, by modulating the polarization of incident light, it is possible to separate the contributions from the isotropic gas or solution phase, from the surface bound species. A spectral frequency range between 1000 cm-1 and 4000 cm-1 can be acquired. While typical spectra with a good signal to noise ratio can be obtained at elevated pressures of gases in ∼2 min at 4 cm-1 resolution, we have also acquired higher resolution spectra at 0.25 cm-1 with longer acquisition times. By way of verification, CO uptake on a heavily oxidized Ru(0001) sample was studied. As part of this test study, the presence of CO adsorbed on Ru bridge sites was confirmed, in agreement with previous ambient pressure X ray photoelectron spectroscopy studies. In terms of instrument performance, it was also determined that the gas phase contribution from CO could be completely removed even up to pressures close to 100 Torr. A second test study demonstrated the use of the technique for studying morphological properties of a spin coated polymer on a conductive surface. Note that this is a novel application of this technique. In this experiment, the polarization of incident light was modulated manually (vs. through a photoelastic modulator). It was demonstrated, in good agreement with the literature, that the polymer chains preferentially lie parallel with the surface. This PM-IRRAS system is small, modular, and easily reconfigurable. It also features a \"vacuum suitcase\" that allows for the integration of the PM-IRRAS system with the rest of the suite of instrumentation at our laboratory available to external users through the CFN user proposal system.", "gemini": "Bài báo này mô tả thiết kế và cấu tạo của một thiết bị quang phổ hấp thụ phản xạ hồng ngoại điều chế phân cực (PM-IRRAS) nhỏ gọn, \"dễ sử dụng\" tại Trung tâm vật liệu nano chức năng (CFN) của Phòng thí nghiệm quốc gia Brookhaven, cho phép nghiên cứu bề mặt ở áp suất từ chân không cực cao đến 100 Torr. Quang phổ hồng ngoại bề mặt rất phù hợp để nghiên cứu các quá trình này vì tần số dao động của các chất phát màu IR nhạy với bản chất của môi trường liên kết trên bề mặt. Dựa trên các quy tắc chọn lọc bề mặt, bằng cách điều chế phân cực của ánh sáng tới, có thể tách các đóng góp từ pha khí hoặc dung dịch đẳng hướng, từ các loài liên kết bề mặt. Có thể thu được dải tần số phổ từ 1000 cm-1 đến 4000 cm-1. Trong khi có thể thu được các phổ điển hình với tỷ lệ tín hiệu trên nhiễu tốt ở áp suất khí cao trong ∼2 phút ở độ phân giải 4 cm-1, chúng tôi cũng đã thu được các phổ có độ phân giải cao hơn ở 0,25 cm-1 với thời gian thu dài hơn. Để xác minh, quá trình hấp thụ CO trên mẫu Ru(0001) bị oxy hóa nặng đã được nghiên cứu. Như một phần của nghiên cứu thử nghiệm này, sự hiện diện của CO hấp phụ trên các vị trí cầu nối Ru đã được xác nhận, phù hợp với các nghiên cứu quang điện tử tia X áp suất xung quanh trước đây. Về hiệu suất của thiết bị, người ta cũng xác định rằng sự đóng góp của pha khí từ CO có thể bị loại bỏ hoàn toàn ngay cả ở áp suất gần 100 Torr. Một nghiên cứu thử nghiệm thứ hai đã chứng minh việc sử dụng kỹ thuật này để nghiên cứu các tính chất hình thái của polyme phủ spin trên bề mặt dẫn điện. Lưu ý rằng đây là một ứng dụng mới của kỹ thuật này. Trong thí nghiệm này, phân cực của ánh sáng tới được điều chế thủ công (so với thông qua bộ điều chế quang đàn hồi). Người ta đã chứng minh, phù hợp với tài liệu, rằng các chuỗi polyme nằm song song với bề mặt. Hệ thống PM-IRRAS này nhỏ, dạng mô-đun và dễ dàng cấu hình lại. Nó cũng có một \"vali chân không\" cho phép tích hợp hệ thống PM-IRRAS với phần còn lại của bộ thiết bị tại phòng thí nghiệm của chúng tôi có sẵn cho người dùng bên ngoài thông qua hệ thống đề xuất người dùng CFN."} +{"idx": 1920034, "en": "A sample environment to enable real-time X-ray scattering measurements to be recorded during the growth of materials by thermal evaporation in vacuum is presented. The in situ capabilities include studying microstructure development with time or during exposure to different environmental conditions, such as temperature and gas pressure. The chamber provides internal slits and a beam stop, to reduce the background scattering from the X-rays passing through the entrance and exit windows, together with highly controllable flux rates of the evaporants. Initial experiments demonstrate some of the possibilities by monitoring the growth of bathophenanthroline (BPhen), a common molecule used in organic solar cells and organic light emitting diodes, including the development of the microstructure with time and depth within the film. The results show how BPhen nanocrystal structures coarsen at room temperature under vacuum, highlighting the importance of using real time measurements to understand the as-deposited pristine film structure and its development with time. More generally, this sample environment is versatile and can be used for investigation of structure-property relationships in a wide range of vacuum deposited materials and their applications in, for example, optoelectronic devices and energy storage.", "gemini": "Một môi trường mẫu để cho phép ghi các phép đo tán xạ tia X thời gian thực trong quá trình phát triển vật liệu bằng cách bốc hơi nhiệt trong chân không được trình bày. Các khả năng tại chỗ bao gồm nghiên cứu sự phát triển vi cấu trúc theo thời gian hoặc trong quá trình tiếp xúc với các điều kiện môi trường khác nhau, chẳng hạn như nhiệt độ và áp suất khí. Buồng cung cấp các khe hở bên trong và một bộ dừng chùm tia, để giảm tán xạ nền từ tia X đi qua cửa sổ vào và ra, cùng với tốc độ thông lượng có thể kiểm soát cao của chất bay hơi. Các thí nghiệm ban đầu chứng minh một số khả năng bằng cách theo dõi sự phát triển của bathophenanthroline (BPhen), một phân tử phổ biến được sử dụng trong pin mặt trời hữu cơ và điốt phát sáng hữu cơ, bao gồm sự phát triển của vi cấu trúc theo thời gian và độ sâu trong màng. Kết quả cho thấy cấu trúc tinh thể nano BPhen thô hơn ở nhiệt độ phòng trong chân không, làm nổi bật tầm quan trọng của việc sử dụng các phép đo thời gian thực để hiểu cấu trúc màng nguyên thủy khi lắng đọng và sự phát triển của nó theo thời gian. Nói chung, môi trường mẫu này rất linh hoạt và có thể được sử dụng để điều tra mối quan hệ cấu trúc-tính chất trong một loạt các vật liệu lắng đọng chân không và ứng dụng của chúng trong các thiết bị quang điện tử và lưu trữ năng lượng."} +{"idx": 1920035, "en": "Star tracking is the primary operating mode of star sensors. To improve tracking accuracy and efficiency, a hybrid method using a star sensor and gyroscopes is proposed in this study. In this method, the dynamic conditions of an aircraft are determined first by the estimated angular acceleration. Under low dynamic conditions, the star sensor is used to measure the star vector and the vector difference method is adopted to estimate the current angular velocity. Under high dynamic conditions, the angular velocity is obtained by the calibrated gyros. The star position is predicted based on the estimated angular velocity and calibrated gyros using the star vector measurements. The results of the semi-physical experiment show that this hybrid method is accurate and feasible. In contrast with the star vector difference and gyro-assisted methods, the star position prediction result of the hybrid method is verified to be more accurate in two different cases under the given random noise of the star centroid.", "gemini": "Theo dõi sao là chế độ hoạt động chính của cảm biến sao. Để cải thiện độ chính xác và hiệu quả theo dõi, một phương pháp lai sử dụng cảm biến sao và con quay hồi chuyển được đề xuất trong nghiên cứu này. Trong phương pháp này, các điều kiện động của máy bay được xác định trước tiên bằng gia tốc góc ước tính. Trong điều kiện động thấp, cảm biến sao được sử dụng để đo vectơ sao và phương pháp chênh lệch vectơ được áp dụng để ước tính vận tốc góc hiện tại. Trong điều kiện động cao, vận tốc góc thu được bằng các con quay đã hiệu chuẩn. Vị trí của sao được dự đoán dựa trên vận tốc góc ước tính và các con quay đã hiệu chuẩn bằng cách sử dụng các phép đo vectơ sao. Kết quả của thí nghiệm bán vật lý cho thấy phương pháp lai này chính xác và khả thi. Trái ngược với phương pháp chênh lệch vectơ sao và phương pháp hỗ trợ con quay hồi chuyển, kết quả dự đoán vị trí sao của phương pháp lai được xác minh là chính xác hơn trong hai trường hợp khác nhau dưới nhiễu ngẫu nhiên nhất định của tâm sao."} +{"idx": 1920036, "en": "For the modeling of multiphase chemical reactors or separation processes, it is essential to predict accurately chemical equilibrium data, such as vapor-liquid or liquid-liquid equilibria [M. Šoóš et al., Chem. Eng. PROCESS Process Intensif. 42(4), 273-284 (2003)]. The instruments used in these experiments are typically designed based on previous experiences, and their operation verified based on known equilibria of standard components. However, mass transfer limitations with different chemical systems may be very different, potentially falsifying the measured equilibrium compositions. In this work, computational fluid dynamics is utilized to design and analyze laboratory scale experimental gas-liquid equilibrium cell for the first time to augment the traditional analysis based on plug flow assumption. Two-phase dilutor cell, used for measuring limiting activity coefficients at infinite dilution, is used as a test case for the analysis. The Lagrangian discrete model is used to track each bubble and to study the residence time distribution of the carrier gas bubbles in the dilutor cell. This analysis is necessary to assess whether the gas leaving the cell is in equilibrium with the liquid, as required in traditional analysis of such apparatus. Mass transfer for six different bio-oil compounds is calculated to determine the approach equilibrium concentration. Also, residence times assuming plug flow and ideal mixing are used as reference cases to evaluate the influence of mixing on the approach to equilibrium in the dilutor. Results show that the model can be used to predict the dilutor operating conditions for which each of the studied gas-liquid systems reaches equilibrium.", "gemini": "Để mô hình hóa các lò phản ứng hóa học đa pha hoặc quá trình tách, việc dự đoán chính xác dữ liệu cân bằng hóa học, chẳng hạn như cân bằng hơi-lỏng hoặc cân bằng lỏng-lỏng là rất cần thiết [M. Šoóš et al., Chem. Eng. PROCESS Process Intensif. 42(4), 273-284 (2003)]. Các dụng cụ được sử dụng trong các thí nghiệm này thường được thiết kế dựa trên kinh nghiệm trước đó và hoạt động của chúng được xác minh dựa trên các cân bằng đã biết của các thành phần tiêu chuẩn. Tuy nhiên, các hạn chế về truyền khối với các hệ thống hóa học khác nhau có thể rất khác nhau, có khả năng làm sai lệch các thành phần cân bằng được đo. Trong công trình này, động lực học chất lỏng tính toán được sử dụng để thiết kế và phân tích tế bào cân bằng khí-lỏng thử nghiệm ở quy mô phòng thí nghiệm lần đầu tiên để tăng cường phân tích truyền thống dựa trên giả định dòng phích. Tế bào pha loãng hai pha, được sử dụng để đo các hệ số hoạt động giới hạn ở độ pha loãng vô hạn, được sử dụng làm trường hợp thử nghiệm để phân tích. Mô hình rời rạc Lagrangian được sử dụng để theo dõi từng bong bóng và nghiên cứu phân bố thời gian lưu của các bong bóng khí mang trong tế bào pha loãng. Phân tích này là cần thiết để đánh giá xem khí thoát ra khỏi tế bào có cân bằng với chất lỏng hay không, như yêu cầu trong phân tích truyền thống của thiết bị như vậy. Truyền khối cho sáu hợp chất dầu sinh học khác nhau được tính toán để xác định nồng độ cân bằng tiếp cận. Ngoài ra, thời gian lưu giả sử dòng phích và trộn lý tưởng được sử dụng làm trường hợp tham chiếu để đánh giá ảnh hưởng của việc trộn đối với phương pháp tiếp cận cân bằng trong chất pha loãng. Kết quả cho thấy mô hình có thể được sử dụng để dự đoán các điều kiện vận hành pha loãng mà mỗi hệ thống khí-lỏng được nghiên cứu đạt đến trạng thái cân bằng."} +{"idx": 1920037, "en": "The present paper describes the findings of the calibration of the neutron yield monitors on the Joint European Torus (JET) performed in 2013 using a 252Cf source deployed inside the torus by the remote handling system, with particular regard to the calibration of fission chambers which provide the time resolved neutron yield from JET plasmas. The experimental data obtained in toroidal, radial, and vertical scans are presented. These data are first analysed following an analytical approach adopted in the previous neutron calibrations at JET. In this way, a calibration function for the volumetric plasma source is derived which allows us to understand the importance of the different plasma regions and of different spatial profiles of neutron emissivity on fission chamber response. Neutronics analyses have also been performed to calculate the correction factors needed to derive the plasma calibration factors taking into account the different energy spectrum and angular emission distribution of the calibrating (point) 252Cf source, the discrete positions compared to the plasma volumetric source, and the calibration circumstances. All correction factors are presented and discussed. We discuss also the lessons learnt which are the basis for the on-going 14 MeV neutron calibration at JET and for ITER.", "gemini": "Bài báo hiện tại mô tả các phát hiện về hiệu chuẩn màn hình đo năng suất neutron trên Vòng tròn châu Âu chung (JET) được thực hiện vào năm 2013 bằng cách sử dụng nguồn 252Cf được triển khai bên trong vòng tròn bởi hệ thống xử lý từ xa, đặc biệt liên quan đến hiệu chuẩn các buồng phân hạch cung cấp năng suất neutron được giải quyết theo thời gian từ huyết tương JET. Dữ liệu thực nghiệm thu được trong các lần quét hình xuyến, xuyên tâm và theo chiều dọc được trình bày. Đầu tiên, các dữ liệu này được phân tích theo phương pháp tiếp cận phân tích được áp dụng trong các hiệu chuẩn neutron trước đó tại JET. Theo cách này, một hàm hiệu chuẩn cho nguồn huyết tương thể tích được suy ra cho phép chúng ta hiểu tầm quan trọng của các vùng huyết tương khác nhau và các cấu hình không gian khác nhau của độ phát xạ neutron đối với phản ứng của buồng phân hạch. Các phân tích neutron cũng đã được thực hiện để tính toán các hệ số hiệu chỉnh cần thiết để suy ra các hệ số hiệu chuẩn huyết tương có tính đến phổ năng lượng và phân bố phát xạ góc khác nhau của nguồn hiệu chuẩn (điểm) 252Cf, các vị trí rời rạc so với nguồn thể tích huyết tương và hiệu chuẩn hoàn cảnh. Tất cả các hệ số hiệu chỉnh được trình bày và thảo luận. Chúng tôi cũng thảo luận về những bài học kinh nghiệm là cơ sở cho hiệu chuẩn neutron 14 MeV đang diễn ra tại JET và cho ITER."} +{"idx": 1920038, "en": "A new steady-state apparatus is designed and constructed for the measurement of thermal conductivity (up to 25 W/mK) on a square specimen (300 mm side) with a heating temperature range from 30 °C to 900 °C. A vacuum container, of which the pressure can reach to 1 Pa, is also built for materials which can be easily oxidized. The structure of the facility is different from that of traditional steady-state devices, especially for the design of heating plate and heat sink. To verify the temperature uniformity of the heating plate, a simulation analysis is carried out in this paper. Besides, the heating system, the heat sink, the measuring system, and the vacuum system are presented in detail. In addition, the thermal conductivities of a heat insulation tile, 304L stainless steel, n-docosane, and erythritol are measured by this apparatus. Finally, an uncertainty analysis is discussed depending on different temperatures and materials.", "gemini": "Một thiết bị trạng thái ổn định mới được thiết kế và chế tạo để đo độ dẫn nhiệt (lên đến 25 W/mK) trên một mẫu hình vuông (cạnh 300 mm) với dải nhiệt độ gia nhiệt từ 30 °C đến 900 °C. Một bình chân không, có áp suất có thể đạt tới 1 Pa, cũng được chế tạo cho các vật liệu dễ bị oxy hóa. Cấu trúc của cơ sở khác với cấu trúc của các thiết bị trạng thái ổn định truyền thống, đặc biệt là đối với thiết kế của tấm gia nhiệt và bộ tản nhiệt. Để xác minh tính đồng nhất nhiệt độ của tấm gia nhiệt, một phân tích mô phỏng được thực hiện trong bài báo này. Bên cạnh đó, hệ thống gia nhiệt, bộ tản nhiệt, hệ thống đo lường và hệ thống chân không được trình bày chi tiết. Ngoài ra, độ dẫn nhiệt của một viên ngói cách nhiệt, thép không gỉ 304L, n-docosane và erythritol được đo bằng thiết bị này. Cuối cùng, một phân tích độ không chắc chắn được thảo luận tùy thuộc vào các nhiệt độ và vật liệu khác nhau."} +{"idx": 1920039, "en": "We measure the shock drive capabilities of a 30 J, nanosecond, 527 nm laser system at the matter in extreme conditions hutch of the Linac Coherent Light Source. Using a velocity interferometer system for any reflector, we ascertain the maximum instantaneous ablation pressure and characterize its dependence on a drive laser spot size, spatial profile, and temporal profile. We also examine the effects of these parameters on shock spatial and temporal uniformity. Our analysis shows the drive laser capable of generating instantaneous ablation pressures exceeding 160 GPa while maintaining a 1D shock profile. We find that slope pulses provide higher instantaneous ablation pressures than plateau pulses. Our results show instantaneous ablation pressures comparable to those measured at the Omega Laser Facility in Rochester, NY under similar optical drive parameters. Finally, we analyze how optical laser ablation pressures are compare with known scaling relations, accounting for variable laser wavelengths.", "gemini": "Chúng tôi đo khả năng truyền động xung của hệ thống laser 30 J, nano giây, 527 nm tại hutch vật chất trong điều kiện khắc nghiệt của Nguồn sáng kết hợp Linac. Sử dụng hệ thống giao thoa kế vận tốc cho bất kỳ máy phản xạ nào, chúng tôi xác định áp suất phá hủy tức thời tối đa và mô tả sự phụ thuộc của áp suất này vào kích thước điểm laser truyền động, cấu hình không gian và cấu hình thời gian. Chúng tôi cũng kiểm tra các hiệu ứng của các thông số này đối với tính đồng nhất về không gian và thời gian của xung. Phân tích của chúng tôi cho thấy laser truyền động có khả năng tạo ra áp suất phá hủy tức thời vượt quá 160 GPa trong khi vẫn duy trì cấu hình xung 1D. Chúng tôi thấy rằng các xung dốc cung cấp áp suất phá hủy tức thời cao hơn các xung đỉnh. Kết quả của chúng tôi cho thấy áp suất phá hủy tức thời tương đương với áp suất đo được tại Cơ sở laser Omega ở Rochester, NY trong các thông số truyền động quang học tương tự. Cuối cùng, chúng tôi phân tích cách áp suất phá hủy laser quang học so sánh với các mối quan hệ tỷ lệ đã biết, tính đến các bước sóng laser biến đổi."} +{"idx": 1920040, "en": "We developed a spectro-electrochemical cell for X-ray absorption and X-ray emission spectroscopy, which are element-specific methods to study local electronic structures in the soft X-ray region. In the usual electrochemical measurement setup, the electrode is placed in solution, and the surface/interface region of the electrode is not normally accessible by soft X-rays that have low penetration depth in liquids. To realize soft X-ray observation of electrochemical reactions, a 15-nm-thick Pt layer was deposited on a 150-nm-thick film window with an adhesive 3-nm-thick Ti layer for use as both the working electrode and the separator window between vacuum and a sample liquid under atmospheric pressure. The designed three-electrode electrochemical cell consists of a Pt film on a SiC window, a platinized Pt wire, and a commercial Ag|AgCl electrode as the working, counter, and reference electrodes, respectively. The functionality of the cell was tested by cyclic voltammetry and X-ray absorption and emission spectroscopy. As a demonstration, the electroplating of Pb on the Pt/SiC membrane window was measured by X-ray absorption and real-time monitoring of fluorescence intensity at the O 1s excitation.", "gemini": "Chúng tôi đã phát triển một tế bào quang điện hóa học cho phép hấp thụ tia X và quang phổ phát xạ tia X, đây là các phương pháp đặc hiệu của từng nguyên tố để nghiên cứu cấu trúc điện tử cục bộ trong vùng tia X mềm. Trong thiết lập đo điện hóa thông thường, điện cực được đặt trong dung dịch và vùng bề mặt/giao diện của điện cực thường không thể tiếp cận được bằng tia X mềm có độ sâu thâm nhập thấp trong chất lỏng. Để hiện thực hóa quan sát tia X mềm về các phản ứng điện hóa, một lớp Pt dày 15 nm đã được lắng đọng trên một cửa sổ màng mỏng dày 150 nm với một lớp Ti dày 3 nm làm chất kết dính để sử dụng làm cả điện cực làm việc và cửa sổ ngăn cách giữa chân không và mẫu chất lỏng dưới áp suất khí quyển. Tế bào điện hóa ba điện cực được thiết kế bao gồm một màng Pt trên cửa sổ SiC, một dây Pt được phủ bạch kim và một điện cực Ag|AgCl thương mại làm điện cực làm việc, điện cực đối và điện cực tham chiếu tương ứng. Chức năng của tế bào đã được kiểm tra bằng phương pháp voltampe kế tuần hoàn và quang phổ hấp thụ và phát xạ tia X. Để chứng minh, quá trình mạ điện Pb trên cửa sổ màng Pt/SiC đã được đo bằng phương pháp hấp thụ tia X và theo dõi cường độ huỳnh quang theo thời gian thực tại sự kích thích O 1s."} +{"idx": 1920041, "en": "We present a general approach to thin bulk samples to transparency for experiments in the soft x-ray and extreme ultraviolet spectral range. The method relies on mechanical grinding followed by focused-ion-beam milling. It results in a uniformly thin area of high surface quality, suitable for nanoscale imaging in transmission. In a proof-of-principle experiment, nanoscale magnetic bits on a commercial hard drive glass disk are imaged with a spatial resolution below 30 nm by soft x-ray spectro-holography. Furthermore, we demonstrate imaging of a lithographically patterned test object via absorption contrast. Our approach is suitable for both amorphous and crystalline substrates and has been tested for a variety of common epitaxy growth substrates. Lateral thinning areas in excess of 100 μm2 and a remaining substrate thickness as thin as 150 nm are easily achievable. Our approach allows preserving a previously grown thin film, and from nanofocus electron diffraction, we find no evidence for morphological changes induced by the process, in agreement with numerical simulations of the ion implantation depth distributon. We expect our method to be widely applicable and especially useful for nanoscale imaging of epitaxial thin films.", "gemini": "Chúng tôi trình bày một phương pháp tiếp cận chung đối với các mẫu khối mỏng để đạt được độ trong suốt cho các thí nghiệm trong phạm vi phổ tia cực tím mềm và cực tím. Phương pháp này dựa trên quá trình mài cơ học sau đó là phay chùm ion tập trung. Nó tạo ra một vùng mỏng đồng đều có chất lượng bề mặt cao, phù hợp để chụp ảnh ở cấp độ nano trong truyền dẫn. Trong một thí nghiệm chứng minh nguyên lý, các bit từ tính ở cấp độ nano trên đĩa thủy tinh của ổ cứng thương mại được chụp ảnh với độ phân giải không gian dưới 30 nm bằng phương pháp quang phổ toàn ảnh tia X mềm. Hơn nữa, chúng tôi trình bày hình ảnh của một vật thử được tạo mẫu bằng phương pháp quang khắc thông qua độ tương phản hấp thụ. Phương pháp của chúng tôi phù hợp với cả chất nền vô định hình và tinh thể và đã được thử nghiệm cho nhiều chất nền phát triển epitaxy thông thường. Các vùng mỏng theo chiều ngang lớn hơn 100 μm2 và độ dày chất nền còn lại mỏng tới 150 nm có thể dễ dàng đạt được. Phương pháp của chúng tôi cho phép bảo toàn màng mỏng đã phát triển trước đó và từ nhiễu xạ điện tử lấy nét nano, chúng tôi không tìm thấy bằng chứng về những thay đổi về mặt hình thái do quá trình này gây ra, phù hợp với các mô phỏng số về phân bố độ sâu cấy ion. Chúng tôi hy vọng phương pháp của mình sẽ được áp dụng rộng rãi và đặc biệt hữu ích cho việc chụp ảnh ở cấp độ nano của các màng mỏng epitaxy."} +{"idx": 1920042, "en": "Many experiments on Sandia National Laboratories' Z Pulsed Power Facility-a 30 MA, 100 ns rise-time, pulsed-power driver-use a monochromatic quartz crystal backlighter system at 1.865 keV (Si Heα) or 6.151 keV (Mn Heα) x-ray energy to radiograph an imploding liner (cylindrical tube) or wire array z-pinch. The x-ray source is generated by the Z-Beamlet laser, which provides two 527-nm, 1 kJ, 1-ns laser pulses. Radiographs of imploding, thick-walled beryllium liners at convergence ratios CR above 15 [CR=ri(0)/ri(t)] using the 6.151-keV backlighter system were too opaque to identify the inner radius ri of the liner with high confidence, demonstrating the need for a higher-energy x-ray radiography system. Here, we present a 7.242 keV backlighter system using a Ge(335) spherical crystal with the Co Heα resonance line. This system operates at a similar Bragg angle as the existing 1.865 keV and 6.151 keV backlighters, enhancing our capabilities for two-color, two-frame radiography without modifying the system integration at Z. The first data taken at Z include 6.2-keV and 7.2-keV two-color radiographs as well as radiographs of low-convergence (CR about 4-5), high-areal-density liner implosions.", "gemini": "Nhiều thí nghiệm trên Cơ sở điện năng xung Z của Phòng thí nghiệm quốc gia Sandia - một trình điều khiển điện năng xung 30 MA, thời gian tăng 100 ns, sử dụng hệ thống đèn nền tinh thể thạch anh đơn sắc ở năng lượng tia X 1,865 keV (Si Heα) hoặc 6,151 keV (Mn Heα) để chụp ảnh tia X một lớp lót nổ tung (ống hình trụ) hoặc mảng dây z-pinch. Nguồn tia X được tạo ra bởi tia laser Z-Beamlet, cung cấp hai xung laser 527 nm, 1 kJ, 1 ns. Ảnh chụp tia X của các lớp lót beryllium dày nổ tung ở tỷ lệ hội tụ CR trên 15 [CR = ri (0) / ri (t)] bằng hệ thống đèn nền 6,151 keV quá mờ đục để xác định bán kính bên trong ri của lớp lót với độ tin cậy cao, chứng minh nhu cầu về hệ thống chụp ảnh tia X năng lượng cao hơn. Tại đây, chúng tôi trình bày hệ thống đèn nền 7,242 keV sử dụng tinh thể hình cầu Ge (335) với vạch cộng hưởng Co Heα. Hệ thống này hoạt động ở góc Bragg tương tự như đèn nền 1,865 keV và 6,151 keV hiện có, tăng cường khả năng chụp ảnh tia X hai màu, hai khung hình của chúng tôi mà không cần sửa đổi tích hợp hệ thống tại Z. Dữ liệu đầu tiên thu được tại Z bao gồm ảnh chụp tia X hai màu 6,2 keV và 7,2 keV cũng như ảnh chụp tia X của lớp lót có mật độ diện tích cao, hội tụ thấp (CR khoảng 4-5)."} +{"idx": 1920043, "en": "A scaffold handling device (SHD) has been designed that can switch from gentle suction to positive pressure to lift and place nanofiber scaffolds. In tissue engineering laboratories, delicate fibrous scaffolds, such as electrospun nanofiber scaffolds, are often used as substrates for cell culture. Typical scaffold handling procedures include lifting the scaffolds, moving them from one container to another, sterilization, and loading scaffolds into cell culture plates. Using tweezers to handle the scaffolds can be slow, can damage the scaffolds, and can cause them to wrinkle or fold. Scaffolds may also acquire a static charge which makes them difficult to put down as they cling to tweezers. An SHD has been designed that enables more efficient, gentle lifting, and placement of delicate scaffolds. Most of the parts to make the SHD can be purchased, except for the tip which can be 3D-printed. The SHD enables more reliable handling of nanofiber scaffolds that may improve the consistency of biomanufacturing processes.", "gemini": "Một thiết bị xử lý giàn giáo (SHD) đã được thiết kế có thể chuyển từ lực hút nhẹ sang áp suất dương để nâng và đặt giàn giáo nanofiber. Trong các phòng thí nghiệm về kỹ thuật mô, các giàn giáo sợi tinh tế, chẳng hạn như giàn giáo nanofiber được điện quay, thường được sử dụng làm chất nền cho nuôi cấy tế bào. Các quy trình xử lý giàn giáo thông thường bao gồm nâng giàn giáo, di chuyển chúng từ thùng chứa này sang thùng chứa khác, khử trùng và nạp giàn giáo vào đĩa nuôi cấy tế bào. Sử dụng nhíp để xử lý giàn giáo có thể chậm, có thể làm hỏng giàn giáo và có thể khiến chúng bị nhăn hoặc gấp lại. Giàn giáo cũng có thể tích điện tích tĩnh khiến chúng khó đặt xuống vì chúng bám vào nhíp. Một SHD đã được thiết kế cho phép nâng và đặt giàn giáo tinh tế nhẹ nhàng và hiệu quả hơn. Hầu hết các bộ phận để chế tạo SHD đều có thể mua được, ngoại trừ đầu có thể in 3D. SHD cho phép xử lý giàn giáo nanofiber đáng tin cậy hơn, có thể cải thiện tính nhất quán của các quy trình sản xuất sinh học."} +{"idx": 1920044, "en": "For the quantitative investigation of MeV-photon-induced changes in the refractive indices of bulk semiconductors, a model was established to describe the evolution of the excess carrier density, including the generation and recombination processes. The two key parameters of the evolution model, namely, the summed injection intensity and the gamma intensity curve, were obtained via dose measurements and gamma pulse monitoring, respectively. An interferometric method of measuring instantaneous changes in the refractive index and obtaining real-time measurements of the excess carrier density in bulk materials was successfully implemented. The probe beam was transmitted through a single-mode fiber to form double-beam interference in a slab geometry. Two bulk samples, one consisting of intrinsic GaAs and one of intrinsic ZnO, were tested. The recombination time constant of the intrinsic GaAs sample was found to be approximately 0.6 ns and did not vary distinctly with the photon energy, whereas the ZnO sample's recombination behavior consisted of two components. The short component was evident when short and intense pulses were incident, whereas the long component dominated under long and relatively weak pulses. The method reported in this work can be used to study the excess carrier dynamics induced by pulsed gamma radiation and to investigate the mechanisms of refractive index modulation under pulsed gamma conditions; thus, it is expected to be beneficial for guiding the development of RadOptic systems based on bulk materials.", "gemini": "Đối với việc nghiên cứu định lượng về các thay đổi do photon MeV gây ra đối với các chỉ số khúc xạ của chất bán dẫn khối, một mô hình đã được thiết lập để mô tả sự tiến hóa của mật độ hạt mang dư thừa, bao gồm các quá trình tạo và tái hợp. Hai tham số chính của mô hình tiến hóa, cụ thể là cường độ phun tổng hợp và đường cong cường độ gamma, lần lượt thu được thông qua phép đo liều lượng và giám sát xung gamma. Một phương pháp giao thoa để đo các thay đổi tức thời trong chỉ số khúc xạ và thu được các phép đo thời gian thực về mật độ hạt mang dư thừa trong vật liệu khối đã được thực hiện thành công. Chùm tia thăm dò được truyền qua sợi quang đơn mode để tạo giao thoa chùm tia kép trong hình học tấm. Hai mẫu khối, một mẫu gồm GaAs nội tại và một mẫu ZnO nội tại, đã được thử nghiệm. Hằng số thời gian tái hợp của mẫu GaAs nội tại được tìm thấy là khoảng 0,6 ns và không thay đổi rõ rệt theo năng lượng photon, trong khi hành vi tái hợp của mẫu ZnO bao gồm hai thành phần. Thành phần ngắn thể hiện rõ khi có các xung ngắn và cường độ cao, trong khi thành phần dài chiếm ưu thế trong các xung dài và tương đối yếu. Phương pháp được báo cáo trong công trình này có thể được sử dụng để nghiên cứu động lực học của hạt mang dư thừa do bức xạ gamma xung gây ra và để nghiên cứu các cơ chế điều chế chỉ số khúc xạ trong điều kiện gamma xung; do đó, phương pháp này dự kiến sẽ có lợi cho việc hướng dẫn phát triển các hệ thống RadOptic dựa trên vật liệu khối."} +{"idx": 1920045, "en": "We describe the hardware, gateware, and software developed at Raytheon BBN Technologies for dynamic quantum information processing experiments on superconducting qubits. In dynamic experiments, real-time qubit state information is fed back or fed forward within a fraction of the qubits' coherence time to dynamically change the implemented sequence. The hardware presented here covers both control and readout of superconducting qubits. For readout, we created a custom signal processing gateware and software stack on commercial hardware to convert pulses in a heterodyne receiver into qubit state assignments with minimal latency, alongside data taking capability. For control, we developed custom hardware with gateware and software for pulse sequencing and steering information distribution that is capable of arbitrary control flow in a fraction of superconducting qubit coherence times. Both readout and control platforms make extensive use of field programmable gate arrays to enable tailored qubit control systems in a reconfigurable fabric suitable for iterative development.", "gemini": "Chúng tôi mô tả phần cứng, phần mềm cổng và phần mềm được phát triển tại Raytheon BBN Technologies cho các thí nghiệm xử lý thông tin lượng tử động trên qubit siêu dẫn. Trong các thí nghiệm động, thông tin trạng thái qubit thời gian thực được đưa trở lại hoặc đưa tới trong một phần thời gian kết hợp của qubit để thay đổi động chuỗi đã thực hiện. Phần cứng được trình bày ở đây bao gồm cả điều khiển và đọc dữ liệu qubit siêu dẫn. Để đọc, chúng tôi đã tạo một phần mềm cổng xử lý tín hiệu tùy chỉnh và ngăn xếp phần mềm trên phần cứng thương mại để chuyển các xung trong bộ thu dị tần thành các phép gán trạng thái qubit với độ trễ tối thiểu, cùng với khả năng tiếp nhận dữ liệu. Để kiểm soát, chúng tôi đã phát triển phần cứng tùy chỉnh với phần mềm cổng và phần mềm để lập trình xung và định hướng phân phối thông tin có khả năng điều khiển luồng tùy ý trong một phần thời gian kết hợp qubit siêu dẫn. Cả nền tảng đọc và kiểm soát đều sử dụng rộng rãi các mảng cổng lập trình được để cho phép các hệ thống điều khiển qubit được thiết kế riêng trong một cấu trúc có thể định cấu hình lại phù hợp với phát triển theo từng bước."} +{"idx": 1920046, "en": "In support of our planar pulsed inductive plasma thruster research, a fast electromagnetic inductive valve for a gas propellant injection system has been built and tested. A new and important design feature is the use of a conical diaphragm as the action part, which greatly contributes to the virtue of simplicity for adopting the resultant force of the diaphragm deformation as the closing force. An optical transmission technique is adopted to measure the opening and closing characters of the valve while the gas throughput is determined by measuring the pressure change per pulse in a test chamber with a capacitance manometer. The experimental results revealed that the delay time before the valve reaction is less than 40 μs, and the valve pulse width is no longer than 160 μs full width at half maximum. The valve delivers 0-2.5 mg of argon gas per pulse varied by adjusting the drive voltage and gas pressure.", "gemini": "Để hỗ trợ nghiên cứu về động cơ đẩy plasma cảm ứng xung mặt phẳng của chúng tôi, một van cảm ứng điện từ nhanh cho hệ thống phun chất đẩy khí đã được chế tạo và thử nghiệm. Một đặc điểm thiết kế mới và quan trọng là sử dụng màng hình nón làm bộ phận tác động, đóng góp rất lớn vào ưu điểm của sự đơn giản khi áp dụng lực kết quả của biến dạng màng ngăn làm lực đóng. Một kỹ thuật truyền quang được áp dụng để đo các đặc tính đóng mở của van trong khi lưu lượng khí được xác định bằng cách đo sự thay đổi áp suất trên mỗi xung trong buồng thử nghiệm bằng đồng hồ đo điện dung. Kết quả thực nghiệm cho thấy thời gian trễ trước phản ứng của van nhỏ hơn 40 μs và độ rộng xung van không dài hơn 160 μs toàn bộ chiều rộng ở nửa cực đại. Van cung cấp 0-2,5 mg khí argon trên mỗi xung thay đổi bằng cách điều chỉnh điện áp truyền động và áp suất khí."} +{"idx": 1920047, "en": "Helicon wave heated plasmas are much more efficient in terms of ionization per unit power consumed. A permanent magnet based compact helicon wave heated plasma source is developed in the Institute for Plasma Research, after carefully optimizing the geometry, the frequency of the RF power, and the magnetic field conditions. The HELicon Experiment for Negative ion-I source is the single driver helicon plasma source that is being studied for the development of a large sized, multi-driver negative hydrogen ion source. In this paper, the details about the single driver machine and the results from the characterization of the device are presented. A parametric study at different pressures and magnetic field values using a 13.56 MHz RF source has been carried out in argon plasma, as an initial step towards source characterization. A theoretical model is also presented for the particle and power balance in the plasma. The ambipolar diffusion process taking place in a magnetized helicon plasma is also discussed.", "gemini": "Các plasma được làm nóng bằng sóng Helicon có hiệu quả hơn nhiều về mặt ion hóa trên mỗi đơn vị công suất tiêu thụ. Một nguồn plasma được làm nóng bằng sóng Helicon nhỏ gọn dựa trên nam châm vĩnh cửu được phát triển tại Viện Nghiên cứu Plasma, sau khi tối ưu hóa cẩn thận hình dạng, tần số của công suất RF và các điều kiện từ trường. Thí nghiệm HELicon cho nguồn ion âm-I là nguồn plasma Helicon một trình điều khiển đang được nghiên cứu để phát triển nguồn ion hydro âm tính đa trình điều khiển có kích thước lớn. Trong bài báo này, các chi tiết về máy một trình điều khiển và kết quả từ việc mô tả đặc điểm của thiết bị được trình bày. Một nghiên cứu tham số ở các áp suất và giá trị từ trường khác nhau bằng cách sử dụng nguồn RF 13,56 MHz đã được thực hiện trong plasma argon, như một bước ban đầu hướng tới việc mô tả đặc điểm của nguồn. Một mô hình lý thuyết cũng được trình bày về cân bằng hạt và công suất trong plasma. Quá trình khuếch tán lưỡng cực diễn ra trong plasma Helicon từ hóa cũng được thảo luận."} +{"idx": 1920048, "en": "We developed a spinner magnetometer to measure the natural remanent magnetization of large Apollo lunar rocks in the storage vault of the Lunar Sample Laboratory Facility (LSLF) of NASA. The magnetometer mainly consists of a commercially available three-axial fluxgate sensor and a hand-rotating sample table with an optical encoder recording the rotation angles. The distance between the sample and the sensor is adjustable according to the sample size and magnetization intensity. The sensor and the sample are placed in a two-layer mu-metal shield to measure the sample natural remanent magnetization. The magnetic signals are acquired together with the rotation angle to obtain stacking of the measured signals over multiple revolutions. The developed magnetometer has a sensitivity of 5 × 10-7 Am2 at the standard sensor-to-sample distance of 15 cm. This sensitivity is sufficient to measure the natural remanent magnetization of almost all the lunar basalt and breccia samples with mass above 10 g in the LSLF vault.", "gemini": "Chúng tôi đã phát triển một máy đo từ kế spinner để đo từ độ tự nhiên còn lại của các loại đá Mặt Trăng Apollo lớn trong kho lưu trữ của Cơ sở Phòng thí nghiệm Mẫu Mặt Trăng (LSLF) của NASA. Máy đo từ kế chủ yếu bao gồm một cảm biến từ thông ba trục có sẵn trên thị trường và một bàn mẫu quay bằng tay với bộ mã hóa quang ghi lại các góc quay. Khoảng cách giữa mẫu và cảm biến có thể điều chỉnh theo kích thước mẫu và cường độ từ hóa. Cảm biến và mẫu được đặt trong một lớp chắn kim loại mu hai lớp để đo từ độ tự nhiên còn lại của mẫu. Các tín hiệu từ được thu lại cùng với góc quay để xếp chồng các tín hiệu đo được trên nhiều vòng quay. Máy đo từ kế đã phát triển có độ nhạy là 5 × 10-7 Am2 ở khoảng cách cảm biến đến mẫu chuẩn là 15 cm. Độ nhạy này đủ để đo từ độ tự nhiên còn lại của hầu hết các mẫu bazan Mặt Trăng và mẫu đá vụn có khối lượng trên 10 g trong kho LSLF."} +{"idx": 1920049, "en": "The extremely low frequency electromagnetic fields (ELF-EMFs) have been shown to have an environmentally negative effect on humans' health; however, its treatment effect is beneficial for patients suffering from neurological disorders. Despite this success, the application of ELF-EMF has exceeded in the understanding of its internal mechanism. Recently, it was found that on-line magnetic stimulation may offer advantages over off-line magnetic exposure and has proven to be effective in activating the prefrontal cortex pyramidal neurons in vitro. Here, we perform computational simulations of the stimulation coils in COMSOL modeling to describe the uniformity of the distribution of the on-line magnetic field. Interestingly, the modeling data and actual measurements showed that the densities of the magnetic flux that was generated by the on-line stimulation coils were similar. The on-line magnetic stimulator induced sodium channel currents as well as field excitatory postsynaptic potentials of the rat hippocampal CA1 neurons and successfully demonstrated its extensive applications to activate neuronal tissue. These findings further raise the possibility that the instrument of on-line magnetic stimulation may be an effective alternative for studies in the field of bioelectromagnetics.", "gemini": "Các trường điện từ tần số cực thấp (ELF-EMF) đã được chứng minh là có tác động tiêu cực đến sức khỏe của con người; tuy nhiên, tác dụng điều trị của nó có lợi cho những bệnh nhân mắc các rối loạn thần kinh. Mặc dù thành công này, việc ứng dụng ELF-EMF đã vượt quá khả năng hiểu biết về cơ chế bên trong của nó. Gần đây, người ta phát hiện ra rằng kích thích từ trực tuyến có thể mang lại lợi thế hơn so với tiếp xúc từ ngoại tuyến và đã được chứng minh là có hiệu quả trong việc kích hoạt các tế bào thần kinh hình chóp vỏ não trước trán trong ống nghiệm. Tại đây, chúng tôi thực hiện mô phỏng tính toán các cuộn dây kích thích trong mô hình COMSOL để mô tả tính đồng đều của sự phân bố của từ trường trực tuyến. Điều thú vị là dữ liệu mô hình và các phép đo thực tế cho thấy mật độ từ thông do các cuộn dây kích thích trực tuyến tạo ra là tương tự nhau. Bộ kích thích từ trực tuyến tạo ra các dòng điện kênh natri cũng như các điện thế sau synap kích thích trường của các tế bào thần kinh CA1 ở hồi hải mã của chuột và đã chứng minh thành công các ứng dụng mở rộng của nó để kích hoạt mô thần kinh. Những phát hiện này càng làm tăng khả năng rằng dụng cụ kích thích từ trực tuyến có thể là một phương pháp thay thế hiệu quả cho các nghiên cứu trong lĩnh vực điện sinh học."} +{"idx": 1920050, "en": "A newly developed method for indication of the bubble state in classical single-bubble cavitation experiments is introduced. The method is based on processing the signal from a sensor, positioned on the flask from outside. The technical means of the method are further explored to improve the position stability of the bubble.", "gemini": "Một phương pháp mới được phát triển để chỉ ra trạng thái bong bóng trong các thí nghiệm tạo hang đơn cổ điển được giới thiệu. Phương pháp này dựa trên việc xử lý tín hiệu từ cảm biến, được đặt ở bên ngoài bình. Các phương tiện kỹ thuật của phương pháp được khám phá thêm để cải thiện độ ổn định vị trí của bong bóng."} +{"idx": 1920051, "en": "We present measurements of ion velocity distributions obtained by laser induced fluorescence (LIF) using a single viewport in an argon plasma. A patent pending design, which we refer to as the confocal fluorescence telescope, combines large objective lenses with a large central obscuration and a spatial filter to achieve high spatial localization along the laser injection direction. Models of the injection and collection optics of the two assemblies are used to provide a theoretical estimate of the spatial localization of the confocal arrangement, which is taken to be the full width at half maximum of the spatial optical response. The new design achieves approximately 1.4 mm localization at a focal length of 148.7 mm, improving on previously published designs by an order of magnitude and approaching the localization achieved by the conventional method. The confocal method, however, does so without requiring a pair of separated, perpendicular optical paths. The confocal technique therefore eases the two window access requirement of the conventional method, extending the application of LIF to experiments where conventional LIF measurements have been impossible or difficult, or where multiple viewports are scarce.", "gemini": "Chúng tôi trình bày các phép đo phân bố vận tốc ion thu được bằng huỳnh quang cảm ứng laser (LIF) sử dụng một cửa sổ duy nhất trong huyết tương argon. Một thiết kế đang chờ cấp bằng sáng chế, mà chúng tôi gọi là kính viễn vọng huỳnh quang đồng tiêu, kết hợp các thấu kính vật kính lớn với một sự che khuất ở tâm lớn và một bộ lọc không gian để đạt được sự định vị không gian cao dọc theo hướng chiếu laser. Các mô hình quang học tiêm và thu của hai cụm được sử dụng để cung cấp ước tính lý thuyết về sự định vị không gian của sự sắp xếp đồng tiêu, được coi là chiều rộng toàn phần ở mức tối đa một nửa của phản ứng quang học không gian. Thiết kế mới đạt được sự định vị khoảng 1,4 mm ở độ dài tiêu cự là 148,7 mm, cải thiện các thiết kế đã công bố trước đó theo cấp độ lớn và đạt được sự định vị đạt được bằng phương pháp thông thường. Tuy nhiên, phương pháp đồng tiêu thực hiện điều đó mà không cần một cặp đường dẫn quang học vuông góc, tách biệt. Do đó, kỹ thuật đồng tiêu làm giảm yêu cầu truy cập hai cửa sổ của phương pháp thông thường, mở rộng ứng dụng LIF cho các thí nghiệm mà các phép đo LIF thông thường là không thể hoặc khó khăn, hoặc khi nhiều cửa sổ là khan hiếm."} +{"idx": 1920052, "en": "When an optical chopper is used to modulate the light source, the rotating speed of the wheel may vary with time and subsequently cause jitter of the modulation frequency. The amplitude calculated from the modulated signal would be distorted when the frequency fluctuations occur. To precisely calculate the amplitude of the modulated light flux, we proposed a method to estimate the range of the frequency fluctuation in the measurement of the spectrum and then extract the amplitude based on the sum of power of the signal in the selected frequency range. Experiments were designed to test the feasibility of the proposed method and the results showed lower root means square error than the conventional way.", "gemini": "Khi sử dụng máy cắt quang để điều chế nguồn sáng, tốc độ quay của bánh xe có thể thay đổi theo thời gian và sau đó gây ra hiện tượng nhiễu tần số điều chế. Biên độ được tính toán từ tín hiệu điều chế sẽ bị méo khi xảy ra biến động tần số. Để tính chính xác biên độ của thông lượng ánh sáng được điều chế, chúng tôi đề xuất một phương pháp để ước tính phạm vi biến động tần số trong phép đo phổ và sau đó trích xuất biên độ dựa trên tổng công suất của tín hiệu trong phạm vi tần số đã chọn. Các thí nghiệm được thiết kế để kiểm tra tính khả thi của phương pháp được đề xuất và kết quả cho thấy lỗi bình phương trung bình thấp hơn so với cách thông thường."} +{"idx": 1920053, "en": "A high precision multi-node clock network for multiple users was built following the precise frequency transmission and time synchronization of 120 km fiber. The network topology adopts a simple star-shaped network structure. The clock signal of a hydrogen maser (synchronized with UTC) was recovered from a 120 km telecommunication fiber link and then was distributed to 4 sub-stations. The fractional frequency instability of all substations is in the level of 10-15 in a second and the clock offset instability is in sub-ps in root-mean-square average.", "gemini": "Một mạng lưới đồng hồ đa nút có độ chính xác cao dành cho nhiều người dùng đã được xây dựng theo tần số truyền chính xác và đồng bộ thời gian của sợi quang 120 km. Cấu trúc mạng lưới áp dụng cấu trúc mạng hình sao đơn giản. Tín hiệu đồng hồ của một maser hydro (đồng bộ với UTC) đã được khôi phục từ một liên kết sợi viễn thông dài 120 km và sau đó được phân phối đến 4 trạm phụ. Độ không ổn định tần số phân số của tất cả các trạm phụ ở mức 10-15 trong một giây và độ không ổn định độ lệch đồng hồ ở mức dưới ps trong giá trị trung bình bình phương căn."} +{"idx": 1920054, "en": "A platform SWE-2 used for single wire explosion experiments has been designed, established, and commissioned. This paper describes the design and initial experiments of SWE-2. In summary, two pulsed current sources based on pulse capacitors and spark gaps are adopted to drive sub-microsecond and microsecond time scale wire explosions in a gaseous/liquid medium, respectively. In the initial experiments, a single copper wire was exploded in air, helium, and argon with a 0.1-0.3 MPa ambient pressure as well as tap water with a 283-323 K temperature, 184-11 000 μS/cm conductivity, or 0.1-0.9 MPa hydrostatic pressure. In addition, the diagnostic system is introduced in detail. Energy deposition, optical emission, and shock wave characteristics are briefly discussed based on experimental results. The platform was demonstrated to operate successfully with a single wire load. These results provide the potential for further applications of this platform, such as plasma-matter interactions, shock wave effects, and reservoir simulations.", "gemini": "Một nền tảng SWE-2 được sử dụng cho các thí nghiệm nổ dây đơn đã được thiết kế, thiết lập và đưa vào sử dụng. Bài báo này mô tả thiết kế và các thí nghiệm ban đầu của SWE-2. Tóm lại, hai nguồn dòng xung dựa trên tụ xung và khe đánh lửa được áp dụng để điều khiển nổ dây thang thời gian dưới microgiây và microgiây trong môi trường khí/lỏng, tương ứng. Trong các thí nghiệm ban đầu, một dây đồng đơn đã được nổ trong không khí, heli và argon với áp suất môi trường xung quanh 0,1-0,3 MPa cũng như nước máy với nhiệt độ 283-323 K, độ dẫn điện 184-11 000 μS/cm hoặc áp suất thủy tĩnh 0,1-0,9 MPa. Ngoài ra, hệ thống chẩn đoán được giới thiệu chi tiết. Sự lắng đọng năng lượng, phát xạ quang học và đặc tính sóng xung kích được thảo luận ngắn gọn dựa trên kết quả thực nghiệm. Nền tảng này đã được chứng minh là hoạt động thành công với tải một dây đơn. Những kết quả này cung cấp tiềm năng cho các ứng dụng tiếp theo của nền tảng này, chẳng hạn như tương tác plasma-vật chất, hiệu ứng sóng xung kích và mô phỏng hồ chứa."} +{"idx": 1920055, "en": "Injection of small (outer diameter = 0.8 mm) plastic pellets carrying embedded smaller (10 μg) tungsten grains is used to check calibrations of core tungsten line radiation diagnostics in support of the 2016 tungsten ring campaign in the DIII-D tokamak. Observed total brightness (1 eV-10 keV) and soft x-ray (1 keV-10 keV) brightness are found to be reasonably well (1 MHz) is of tens of ppm, orders of magnitude worse than the expected value. A detailed analysis shows that the additional noise is due to slow fluctuations of the signal gain from the generation stage to the acquisition one, mainly due to the digital-to-analog and analog-to-digital converters. To compensate them, a switched ratiometric technique based on two analog-to-digital converters alternately acquiring the signal coming from the device under test and the stimulus signal has been conceived. A field-programmabale gate array-based LIA working up to 10 MHz and implementing the technique has been realized, and results demonstrate a resolution improvement of more than an order of magnitude (from tens of ppm down to sub-ppm values) compared to standard implementations working up to similar frequencies. The technique is generally applicable without requiring calibration nor ad hoc experimental arrangements.", "gemini": "Bộ khuếch đại khóa (LIA) được sử dụng rộng rãi để thực hiện các phép đo độ phân giải cao. Về lý thuyết, khi sử dụng LIA, có thể đo được biến thiên tín hiệu tối thiểu bị giới hạn bởi nhiễu tương đương đầu vào của thiết bị tại tần số hoạt động và băng thông lọc đã chọn. Thay vào đó, LIA kỹ thuật số cho thấy nhiễu 1/f không lường trước được ở đầu ra giải điều chế của thiết bị, tỷ lệ thuận với biên độ tín hiệu gây ra giới hạn cơ bản đối với biến thiên tín hiệu tối thiểu có thể phát hiện được khi sử dụng kỹ thuật khóa. Đặc biệt, giới hạn độ phân giải thông thường của LIA hoạt động nhanh (>1 MHz) là hàng chục ppm, kém hơn nhiều lần so với giá trị mong đợi. Một phân tích chi tiết cho thấy nhiễu bổ sung là do dao động chậm của độ lợi tín hiệu từ giai đoạn tạo đến giai đoạn thu nhận, chủ yếu là do bộ chuyển đổi số sang tương tự và tương tự sang số. Để bù đắp cho chúng, một kỹ thuật tỷ lệ chuyển mạch dựa trên hai bộ chuyển đổi tương tự sang số lần lượt thu tín hiệu đến từ thiết bị đang thử nghiệm và tín hiệu kích thích đã được hình thành. Một LIA dựa trên mảng cổng lập trình trường hoạt động lên đến 10 MHz và triển khai kỹ thuật đã được thực hiện và kết quả chứng minh độ phân giải được cải thiện hơn một bậc độ lớn (từ hàng chục ppm xuống giá trị dưới ppm) so với các triển khai tiêu chuẩn hoạt động ở các tần số tương tự. Kỹ thuật này có thể áp dụng chung mà không cần hiệu chuẩn hoặc sắp xếp thử nghiệm đặc biệt."} +{"idx": 1920070, "en": "To enhance the harmonic signals in intermittent contact atomic force microscopy, we proposed the double-hole structural modification. Finite element analyses and experiments demonstrated the capability and advantages of the developed method. An infinite set of harmonic cantilevers can be optimized by proper selections of hole size, position, and inter-distance. The second and third resonance frequencies are simultaneously regulated to be integer multiples of the fundamental frequency. In the meanwhile, the alteration of cantilever stiffness is kept minimum. The double-hole modifications have prominent advantages of regular geometry, flexible selection of cutting positions/dimensions, and easy-to-meet fabrication tolerances.", "gemini": "Để tăng cường các tín hiệu sóng hài trong kính hiển vi lực nguyên tử tiếp xúc không liên tục, chúng tôi đã đề xuất sửa đổi cấu trúc hai lỗ. Các phân tích phần tử hữu hạn và các thí nghiệm đã chứng minh khả năng và lợi thế của phương pháp đã phát triển. Có thể tối ưu hóa một tập vô hạn các thanh treo sóng hài bằng cách lựa chọn kích thước lỗ, vị trí và khoảng cách giữa các lỗ một cách thích hợp. Tần số cộng hưởng thứ hai và thứ ba được điều chỉnh đồng thời để trở thành bội số nguyên của tần số cơ bản. Trong khi đó, độ cứng của thanh treo được giữ ở mức tối thiểu. Các sửa đổi hai lỗ có những ưu điểm nổi bật về hình học thông thường, lựa chọn linh hoạt các vị trí/kích thước cắt và dung sai chế tạo dễ đáp ứng."} +{"idx": 1920071, "en": "In this paper, a removal method of micro/nanoscale particles in a submillimeter-diameter area at the interface between an aqueous suspension droplet and silicon substrate surface around a selectable point is proposed and demonstrated. It employs the acoustic streaming generated by an ultrasonically vibrating micro manipulating probe (MMP). The operating frequency of the device is 124.5 kHz, at which the micro manipulating probe oscillates approximately linearly. The experiments show that microscale particles with a diameter of 3-5 μm and nanoscale particles with a diameter of 300-500 nm in submillimeter-diameter areas can be removed in about 1.5 min. The principle of the cleaning method is analyzed by measuring the device's vibration mode and computing the 3D acoustic streaming field around the MMP. The diameter of cleaned area versus sonication time is clarified by experiments as well as the stable diameter of cleaned area versus vibration velocity. The dependency of the acoustic streaming field on the working parameters, which include the distance between the MMP's tip and substrate, the angle between the MMP and substrate, and the ratio of the normal vibration components of the MMP, is also investigated by the FEM (finite element method) computation. To the best of our knowledge, this is the first time to report a method for the removal of micro/nanoscale particles in a submillimeter range around a selectable point.", "gemini": "Trong bài báo này, một phương pháp loại bỏ các hạt ở cấp độ micro/nano trong một vùng có đường kính dưới milimet tại giao diện giữa giọt huyền phù nước và bề mặt nền silicon xung quanh một điểm có thể lựa chọn được đề xuất và chứng minh. Phương pháp này sử dụng luồng âm thanh được tạo ra bởi đầu dò siêu âm rung siêu nhỏ (MMP). Tần số hoạt động của thiết bị là 124,5 kHz, tại tần số này, đầu dò siêu nhỏ dao động gần như tuyến tính. Các thí nghiệm cho thấy các hạt ở cấp độ micro có đường kính 3-5 μm và các hạt ở cấp độ nano có đường kính 300-500 nm trong các vùng có đường kính dưới milimet có thể được loại bỏ trong khoảng 1,5 phút. Nguyên lý của phương pháp làm sạch được phân tích bằng cách đo chế độ rung của thiết bị và tính toán trường luồng âm thanh 3D xung quanh MMP. Đường kính của vùng được làm sạch so với thời gian siêu âm được làm rõ bằng các thí nghiệm cũng như đường kính ổn định của vùng được làm sạch so với vận tốc rung. Sự phụ thuộc của trường luồng âm thanh vào các thông số làm việc, bao gồm khoảng cách giữa đầu MMP và nền, góc giữa MMP và nền và tỷ lệ các thành phần rung bình thường của MMP, cũng được nghiên cứu bằng phép tính FEM (phương pháp phần tử hữu hạn). Theo hiểu biết của chúng tôi, đây là lần đầu tiên báo cáo một phương pháp loại bỏ các hạt ở cấp độ micro/nano trong phạm vi dưới milimet xung quanh một điểm có thể lựa chọn."} +{"idx": 1920072, "en": "Ring-averaged velocity distribution function of ions at a fixed guiding center position is a fundamental quantity in the gyrokinetic plasma physics. We have developed a diagnostic tool for the ring averaged velocity distribution function of ions for laboratory plasma experiments, which is named as the ring-averaged ion distribution function probe (RIDFP). The RIDFP is a set of ion collectors for different velocities. It is designed to be immersed in magnetized plasmas and achieves momentum selection of incoming ions by the selection of the ion Larmor radii. To nullify the influence of the sheath potential surrounding the RIDFP on the orbits of the incoming ions, the electrostatic potential of the RIDFP body is automatically adjusted to coincide with the space potential of the target plasma with the use of an emissive probe and a voltage follower. The developed RIDFP successfully measured the equilibrium ring-averaged velocity distribution function of a laboratory magnetized plasma, which was in accordance with the Maxwellian distribution having an ion temperature of 0.2 eV.", "gemini": "Hàm phân bố vận tốc trung bình vòng của các ion tại vị trí tâm dẫn hướng cố định là một đại lượng cơ bản trong vật lý plasma động học. Chúng tôi đã phát triển một công cụ chẩn đoán cho hàm phân bố vận tốc trung bình vòng của các ion cho các thí nghiệm plasma trong phòng thí nghiệm, được gọi là đầu dò hàm phân bố ion trung bình vòng (RIDFP). RIDFP là một tập hợp các bộ thu ion cho các vận tốc khác nhau. Nó được thiết kế để nhúng vào các plasma từ hóa và đạt được sự lựa chọn động lượng của các ion tới bằng cách lựa chọn bán kính Larmor của ion. Để vô hiệu hóa ảnh hưởng của điện thế vỏ bao quanh RIDFP lên quỹ đạo của các ion tới, điện thế tĩnh điện của thân RIDFP được tự động điều chỉnh để trùng với điện thế không gian của plasma mục tiêu bằng cách sử dụng đầu dò phát xạ và bộ theo điện áp. RIDFP đã phát triển đã đo thành công hàm phân bố vận tốc trung bình vòng cân bằng của plasma từ hóa trong phòng thí nghiệm, phù hợp với phân bố Maxwellian có nhiệt độ ion là 0,2 eV."} +{"idx": 1920073, "en": "In this paper, we present the design and measured performance of a novel cryogenic motor based on a superconducting magnetic bearing (SMB). The motor is tailored for use in millimeter-wave half-wave plate (HWP) polarimeters, where a HWP is rapidly rotated in front of a polarization analyzer or polarization-sensitive detector. This polarimetry technique is commonly used in cosmic microwave background polarization studies. The SMB we use is composed of fourteen yttrium barium copper oxide (YBCO) disks and a contiguous neodymium iron boron (NdFeB) ring magnet. The motor is a hollow-shaft motor because the HWP is ultimately installed in the rotor. The motor presented here has a 100 mm diameter rotor aperture. However, the design can be scaled up to rotor aperture diameters of approximately 500 mm. Our motor system is composed of four primary subsystems: (i) the rotor assembly, which includes the NdFeB ring magnet, (ii) the stator assembly, which includes the YBCO disks, (iii) an incremental encoder, and (iv) the drive electronics. While the YBCO is cooling through its superconducting transition, the rotor is held above the stator by a novel hold and release mechanism. The encoder subsystem consists of a custom-built encoder disk read out by two fiber optic readout sensors. For the demonstration described in this paper, we ran the motor at 50 K and tested rotation frequencies up to approximately 10 Hz. The feedback system was able to stabilize the rotation speed to approximately 0.4%, and the measured rotor orientation angle uncertainty is less than 0.15°. Lower temperature operation will require additional development activities, which we will discuss.", "gemini": "Trong bài báo này, chúng tôi trình bày thiết kế và hiệu suất được đo của một động cơ lạnh mới dựa trên ổ trục từ siêu dẫn (SMB). Động cơ được thiết kế để sử dụng trong các phân cực kế tấm nửa sóng (HWP) sóng milimet, trong đó HWP được quay nhanh trước bộ phân tích phân cực hoặc bộ dò nhạy phân cực. Kỹ thuật phân cực này thường được sử dụng trong các nghiên cứu phân cực nền vi sóng vũ trụ. SMB mà chúng tôi sử dụng bao gồm mười bốn đĩa oxit đồng bari ytri (YBCO) và một nam châm vòng neodymium sắt bo (NdFeB) liền kề. Động cơ là động cơ trục rỗng vì HWP cuối cùng được lắp vào rôto. Động cơ được trình bày ở đây có khẩu độ rôto đường kính 100 mm. Tuy nhiên, thiết kế có thể được mở rộng đến các đường kính khẩu độ rôto khoảng 500 mm. Hệ thống động cơ của chúng tôi bao gồm bốn hệ thống con chính: (i) cụm rôto, bao gồm nam châm vòng NdFeB, (ii) cụm stato, bao gồm các đĩa YBCO, (iii) bộ mã hóa gia tăng và (iv) bộ truyền động điện tử. Trong khi YBCO đang làm mát qua quá trình chuyển đổi siêu dẫn, rôto được giữ trên stato bằng cơ chế giữ và nhả mới. Hệ thống con mã hóa bao gồm một đĩa mã hóa được chế tạo riêng được đọc bởi hai cảm biến đọc sợi quang. Đối với bản trình diễn được mô tả trong bài báo này, chúng tôi đã chạy động cơ ở 50 K và thử nghiệm các tần số quay lên đến khoảng 10 Hz. Hệ thống phản hồi có thể ổn định tốc độ quay ở mức khoảng 0,4% và độ không chắc chắn về góc định hướng rôto được đo được nhỏ hơn 0,15°. Hoạt động ở nhiệt độ thấp hơn sẽ yêu cầu các hoạt động phát triển bổ sung, chúng tôi sẽ thảo luận."} +{"idx": 1920074, "en": "Based on the Littrow and Littman configurations for autocollimation, two methods are proposed herein with a fore-end light path comprising fused fiber couplers and fiber port collimators to measure the diffraction grating period. The experimental data from a uniform-line-spacing grating with a nominal value of 1200 lines/mm agreed well with theoretical values and with measurement errors of the Littrow and the Littman configurations of 1.05 × 10-4 and 4.99 × 10-5, respectively. Both methods were demonstrated to reduce the diameter of the laser spot and improve the alignment precision of the measurement system.", "gemini": "Dựa trên các cấu hình Littrow và Littman để tự chuẩn trực, tại đây chúng tôi đề xuất hai phương pháp với đường dẫn ánh sáng phía trước bao gồm bộ ghép sợi hợp nhất và bộ chỉnh độ sợi để đo chu kỳ mạng nhiễu xạ. Dữ liệu thực nghiệm từ một mạng có khoảng cách đường thẳng đồng đều với giá trị danh nghĩa là 1200 vạch/mm phù hợp với các giá trị lý thuyết và với sai số đo của cấu hình Littrow và cấu hình Littman lần lượt là 1,05 × 10-4 và 4,99 × 10-5. Cả hai phương pháp đều được chứng minh là làm giảm đường kính của điểm laser và cải thiện độ chính xác căn chỉnh của hệ thống đo lường."} +{"idx": 1920075, "en": "There is a considerable interest to investigate surface crystallization in order to have a full mechanistic understanding of how layers of sparingly soluble salts (scale) build on component surfaces. Despite much recent attention, a suitable methodology to improve on the understanding of the precipitation/deposition systems to enable the construction of an accurate surface deposition kinetic model is still needed. In this work, an experimental flow rig and associated methodology to study mineral scale deposition is developed. The once-through flow rig allows us to follow mineral scale precipitation and surface deposition in situ and in real time. The rig enables us to assess the effects of various parameters such as brine chemistry and scaling indices, temperature, flow rates, and scale inhibitor concentrations on scaling kinetics. Calcium carbonate (CaCO3) scaling at different values of the saturation ratio (SR) is evaluated using image analysis procedures that enable the assessment of surface coverage, nucleation, and growth of the particles with time. The result for turbidity values measured in the flow cell is zero for all the SR considered. The residence time from the mixing point to the sample is shorter than the induction time for bulk precipitation; therefore, there are no crystals in the bulk solution as the flow passes through the sample. The study shows that surface scaling is not always a result of pre-precipitated crystals in the bulk solution. The technique enables both precipitation and surface deposition of scale to be decoupled and for the surface deposition process to be studied in real time and assessed under constant condition.", "gemini": "Có một sự quan tâm đáng kể đến việc nghiên cứu quá trình kết tinh bề mặt để có được sự hiểu biết đầy đủ về cơ chế của cách các lớp muối ít tan (cặn) tích tụ trên bề mặt thành phần. Mặc dù gần đây có nhiều sự chú ý, nhưng vẫn cần một phương pháp luận phù hợp để cải thiện sự hiểu biết về hệ thống kết tủa/bám cặn để có thể xây dựng mô hình động học lắng cặn bề mặt chính xác. Trong nghiên cứu này, một giàn dòng chảy thử nghiệm và phương pháp luận liên quan để nghiên cứu quá trình lắng cặn cặn khoáng đã được phát triển. Giàn dòng chảy một lần cho phép chúng ta theo dõi quá trình kết tủa cặn khoáng và lắng cặn bề mặt tại chỗ và theo thời gian thực. Giàn cho phép chúng ta đánh giá các tác động của các thông số khác nhau như hóa học nước muối và chỉ số đóng cặn, nhiệt độ, tốc độ dòng chảy và nồng độ chất ức chế cặn đối với động học đóng cặn. Quá trình đóng cặn canxi cacbonat (CaCO3) ở các giá trị khác nhau của tỷ lệ bão hòa (SR) được đánh giá bằng các quy trình phân tích hình ảnh cho phép đánh giá độ phủ bề mặt, sự hình thành và phát triển của các hạt theo thời gian. Kết quả đối với các giá trị độ đục đo được trong ô dòng chảy là không đối với tất cả các SR được xem xét. Thời gian lưu trú từ điểm trộn đến mẫu ngắn hơn thời gian cảm ứng để kết tủa khối; do đó, không có tinh thể nào trong dung dịch khối khi dòng chảy đi qua mẫu. Nghiên cứu cho thấy rằng sự đóng cặn bề mặt không phải lúc nào cũng là kết quả của các tinh thể kết tủa trước trong dung dịch khối. Kỹ thuật này cho phép tách biệt cả quá trình kết tủa và lắng cặn bề mặt của cặn và để quá trình lắng cặn bề mặt được nghiên cứu theo thời gian thực và đánh giá trong điều kiện không đổi."} +{"idx": 1920076, "en": "Commonly used thermal analysis tools such as calorimeter and thermal conductivity meter are separated instruments and limited by low throughput, where only one sample is examined each time. This work reports an infrared based optical calorimetry with its theoretical foundation, which is able to provide an integrated solution to characterize thermal properties of materials with high throughput. By taking time domain temperature information of spatially distributed samples, this method allows a single device (infrared camera) to determine the thermal properties of both phase change systems (melting temperature and latent heat of fusion) and non-phase change systems (thermal conductivity and heat capacity). This method further allows these thermal properties of multiple samples to be determined rapidly, remotely, and simultaneously. In this proof-of-concept experiment, the thermal properties of a panel of 16 samples including melting temperatures, latent heats of fusion, heat capacities, and thermal conductivities have been determined in 2 min with high accuracy. Given the high thermal, spatial, and temporal resolutions of the advanced infrared camera, this method has the potential to revolutionize the thermal characterization of materials by providing an integrated solution with high throughput, high sensitivity, and short analysis time.", "gemini": "Các công cụ phân tích nhiệt thường được sử dụng như máy đo nhiệt lượng và máy đo độ dẫn nhiệt là các thiết bị riêng biệt và bị hạn chế bởi thông lượng thấp, trong đó chỉ có một mẫu được kiểm tra mỗi lần. Nghiên cứu này báo cáo về phép đo nhiệt lượng quang học dựa trên tia hồng ngoại với nền tảng lý thuyết của nó, có khả năng cung cấp giải pháp tích hợp để mô tả các tính chất nhiệt của vật liệu với thông lượng cao. Bằng cách lấy thông tin nhiệt độ miền thời gian của các mẫu phân bố không gian, phương pháp này cho phép một thiết bị duy nhất (camera hồng ngoại) xác định các tính chất nhiệt của cả hệ thống thay đổi pha (nhiệt độ nóng chảy và nhiệt nóng chảy tiềm ẩn) và hệ thống không thay đổi pha (độ dẫn nhiệt và nhiệt dung). Phương pháp này cho phép các tính chất nhiệt của nhiều mẫu này được xác định nhanh chóng, từ xa và đồng thời. Trong thí nghiệm chứng minh khái niệm này, các tính chất nhiệt của một bảng gồm 16 mẫu bao gồm nhiệt độ nóng chảy, nhiệt nóng chảy tiềm ẩn, nhiệt dung và độ dẫn nhiệt đã được xác định trong 2 phút với độ chính xác cao. Với độ phân giải nhiệt, không gian và thời gian cao của camera hồng ngoại tiên tiến, phương pháp này có khả năng cách mạng hóa đặc tính nhiệt của vật liệu bằng cách cung cấp giải pháp tích hợp với thông lượng cao, độ nhạy cao và thời gian phân tích ngắn."} +{"idx": 1920077, "en": "In situ measurements are an increasingly important tool to inform the complex relationship between nanoscale properties and macroscopic material measurements. Knowledge of these phenomena can be used to develop new materials to meet the performance demands of next generation technologies. Conductive complex fluids have emerged as an area of research where the electrical and mechanical properties are key design parameters. To study the relationship between microstructure, conductivity, and rheology, we have developed a small angle neutron scattering (SANS) compatible Couette rheological geometry capable of making impedance spectroscopy measurements under continuous shear. We have also mounted this geometry on a commercial strain controlled rheometer with a modified forced convection oven. In this manuscript, we introduce the simultaneous measurement of impedance spectroscopy, rheological properties and SANS data. We describe the validation of this dielectric RheoSANS instrument and demonstrate its operation using two systems-an ion gel comprising Pluronic® surfactant and ionic liquid, ethyl-ammonium nitrate, and poly(3-hexylthiophene) organogel prepared in a mixture of hexadecane and dichlorobenzene. In both systems, we use this new measurement capability to study the microstructural state of these materials under two different protocols. By monitoring their dielectric rheology at the same time as the SANS measurement, we demonstrate the capacity to directly probe structure-property relationships inherent to the macroscopic material response.", "gemini": "Các phép đo tại chỗ là một công cụ ngày càng quan trọng để cung cấp thông tin về mối quan hệ phức tạp giữa các tính chất ở cấp độ nano và các phép đo vật liệu vĩ mô. Kiến thức về các hiện tượng này có thể được sử dụng để phát triển các vật liệu mới đáp ứng nhu cầu hiệu suất của các công nghệ thế hệ tiếp theo. Các chất lỏng phức tạp dẫn điện đã nổi lên như một lĩnh vực nghiên cứu trong đó các tính chất điện và cơ học là các thông số thiết kế chính. Để nghiên cứu mối quan hệ giữa cấu trúc vi mô, độ dẫn điện và lưu biến học, chúng tôi đã phát triển hình học lưu biến Couette tương thích với tán xạ neutron góc nhỏ (SANS) có khả năng thực hiện các phép đo phổ trở kháng dưới lực cắt liên tục. Chúng tôi cũng đã lắp hình học này trên máy đo lưu biến điều khiển biến dạng thương mại với lò đối lưu cưỡng bức đã được sửa đổi. Trong bản thảo này, chúng tôi giới thiệu phép đo đồng thời phổ trở kháng, tính chất lưu biến và dữ liệu SANS. Chúng tôi mô tả việc xác nhận thiết bị RheoSANS điện môi này và trình bày hoạt động của nó bằng cách sử dụng hai hệ thống - một loại gel ion bao gồm chất hoạt động bề mặt Pluronic® và chất lỏng ion, ethyl-amoni nitrat và organogel poly(3-hexylthiophene) được chế tạo trong hỗn hợp hexadecan và diclobenzen. Trong cả hai hệ thống, chúng tôi sử dụng khả năng đo lường mới này để nghiên cứu trạng thái vi cấu trúc của các vật liệu này theo hai giao thức khác nhau. Bằng cách theo dõi lưu biến điện môi của chúng cùng lúc với phép đo SANS, chúng tôi chứng minh khả năng thăm dò trực tiếp các mối quan hệ cấu trúc-tính chất vốn có trong phản ứng vật liệu vĩ mô."} +{"idx": 1920078, "en": "Angular filter refractometry (AFR) is a novel technique used to characterize the density profiles of laser-produced, long-scale-length plasmas [Haberberger et al., Phys. Plasmas 21, 056304 (2014)]. A new method of analysis for AFR images was developed using an annealing algorithm to iteratively converge upon a solution. A synthetic AFR image is constructed by a user-defined density profile described by eight parameters, and the algorithm systematically alters the parameters until the comparison is optimized. The optimization and statistical uncertainty calculation is based on the minimization of the χ2 test statistic. The algorithm was successfully applied to experimental data of plasma expanding from a flat, laser-irradiated target, resulting in an average uncertainty in the density profile of 5%-20% in the region of interest.", "gemini": "Kỹ thuật khúc xạ lọc góc (AFR) là một kỹ thuật mới được sử dụng để mô tả các cấu hình mật độ của plasma có chiều dài quy mô lớn do laser tạo ra [Haberberger et al., Phys. Plasmas 21, 056304 (2014)]. Một phương pháp phân tích mới đối với hình ảnh AFR đã được phát triển bằng cách sử dụng thuật toán ủ để hội tụ theo từng bước đến một giải pháp. Một hình ảnh AFR tổng hợp được xây dựng bằng cấu hình mật độ do người dùng xác định được mô tả bởi tám thông số và thuật toán sẽ thay đổi các thông số một cách có hệ thống cho đến khi tối ưu hóa được phép so sánh. Tối ưu hóa và tính toán độ không chắc chắn về mặt thống kê dựa trên việc giảm thiểu thống kê kiểm tra χ2. Thuật toán đã được áp dụng thành công vào dữ liệu thực nghiệm về plasma mở rộng từ mục tiêu phẳng được chiếu laser, dẫn đến độ không chắc chắn trung bình trong cấu hình mật độ là 5%-20% trong khu vực quan tâm."} +{"idx": 1920079, "en": "Brain-computer interfaces have been a novel approach to translate human intentions into movement commands in robotic systems. This paper describes an electroencephalogram-based brain-controlled lower-limb exoskeleton for gait training, as a proof of concept towards rehabilitation with human-in-the-loop. Instead of using conventional single electroencephalography correlates, e.g., evoked P300 or spontaneous motor imagery, we propose a novel framework integrated two asynchronous signal modalities, i.e., sensorimotor rhythms (SMRs) and movement-related cortical potentials (MRCPs). We executed experiments in a biologically inspired and customized lower-limb exoskeleton where subjects (N = 6) actively controlled the robot using their brain signals. Each subject performed three consecutive sessions composed of offline training, online visual feedback testing, and online robot-control recordings. Post hoc evaluations were conducted including mental workload assessment, feature analysis, and statistics test. An average robot-control accuracy of 80.16% ± 5.44% was obtained with the SMR-based method, while estimation using the MRCP-based method yielded an average performance of 68.62% ± 8.55%. The experimental results showed the feasibility of the proposed framework with all subjects successfully controlled the exoskeleton. The current paradigm could be further extended to paraplegic patients in clinical trials.", "gemini": "Giao diện não-máy tính đã trở thành một phương pháp mới để chuyển đổi ý định của con người thành các lệnh chuyển động trong hệ thống rô bốt. Bài báo này mô tả một bộ xương ngoài chi dưới được điều khiển bằng não dựa trên điện não đồ để phục hồi chức năng đi lại, như một bằng chứng về khái niệm hướng tới phục hồi chức năng với sự tham gia của con người. Thay vì sử dụng các tương quan điện não đồ đơn thông thường, ví dụ, P300 được gợi ra hoặc hình ảnh vận động tự phát, chúng tôi đề xuất một khuôn khổ mới tích hợp hai phương thức tín hiệu không đồng bộ, tức là nhịp vận động cảm giác (SMR) và điện thế vỏ não liên quan đến vận động (MRCP). Chúng tôi đã thực hiện các thí nghiệm trong một bộ xương ngoài chi dưới được tùy chỉnh và lấy cảm hứng từ sinh học, trong đó các đối tượng (N = 6) chủ động điều khiển rô bốt bằng tín hiệu não của họ. Mỗi đối tượng thực hiện ba phiên liên tiếp bao gồm đào tạo ngoại tuyến, kiểm tra phản hồi trực quan trực tuyến và ghi lại điều khiển rô bốt trực tuyến. Đánh giá sau thí nghiệm đã được tiến hành bao gồm đánh giá khối lượng công việc về mặt tinh thần, phân tích tính năng và kiểm tra thống kê. Độ chính xác điều khiển rô bốt trung bình là 80,16% ± 5,44% thu được bằng phương pháp dựa trên SMR, trong khi ước tính sử dụng phương pháp dựa trên MRCP cho hiệu suất trung bình là 68,62% ± 8,55%. Kết quả thực nghiệm cho thấy tính khả thi của khuôn khổ được đề xuất với tất cả các đối tượng đều điều khiển thành công bộ xương ngoài. Mô hình hiện tại có thể được mở rộng thêm cho bệnh nhân liệt nửa người trong các thử nghiệm lâm sàng."} +{"idx": 1920080, "en": "This paper describes the development and testing of a novel capillary flow cell for use in in situ powder X-ray diffraction experiments. It is designed such that it achieves 200° of rotation of the capillary whilst still allowing the flow of gas through the sample and the monitoring of off gas via mass spectrometry, gas chromatography, or other such analytical techniques. This high degree of rotation provides more uniform heating of the sample than can be achieved in static cells or those with lower rotational ranges and consequently also improves particle statistics. The increased uniformity of heating provides more accurate temperature calibration of the experimental setup as well. The cell is designed to be held in a standard goniometer head and is therefore suitable for use in many laboratory and synchrotron instruments.", "gemini": "Bài báo này mô tả quá trình phát triển và thử nghiệm một tế bào dòng mao quản mới để sử dụng trong các thí nghiệm nhiễu xạ tia X bột tại chỗ. Nó được thiết kế để đạt được góc quay 200° của mao quản trong khi vẫn cho phép dòng khí đi qua mẫu và theo dõi khí thải thông qua phổ khối, sắc ký khí hoặc các kỹ thuật phân tích khác. Mức độ quay cao này cung cấp khả năng gia nhiệt đồng đều hơn cho mẫu so với các tế bào tĩnh hoặc các tế bào có dải quay thấp hơn và do đó cũng cải thiện thống kê hạt. Sự đồng đều gia nhiệt tăng lên cũng cung cấp hiệu chuẩn nhiệt độ chính xác hơn cho thiết lập thực nghiệm. Tế bào được thiết kế để được giữ trong đầu goniometer tiêu chuẩn và do đó phù hợp để sử dụng trong nhiều thiết bị phòng thí nghiệm và máy gia tốc."} +{"idx": 1920081, "en": "We present the design and operating characteristics of a vacuum furnace used for inelastic neutron scattering experiments on a time-of-flight chopper spectrometer. The device is an actively water cooled radiant heating furnace capable of performing experiments up to 1873 K. Inelastic neutron scattering studies performed with this furnace include studies of phonon dynamics and metallic liquids. We describe the design, control, characterization, and limitations of the equipment. Further, we provide comparisons of the neutron performance of our device with commercially available options. Finally we consider upgrade paths to improve performance and reliability.", "gemini": "Chúng tôi trình bày thiết kế và đặc điểm hoạt động của lò chân không được sử dụng cho các thí nghiệm tán xạ neutron phi đàn hồi trên máy quang phổ chặt thời gian bay. Thiết bị này là lò nung bức xạ làm mát bằng nước hoạt động có khả năng thực hiện các thí nghiệm lên đến 1873 K. Các nghiên cứu tán xạ neutron phi đàn hồi được thực hiện với lò nung này bao gồm các nghiên cứu về động lực phonon và chất lỏng kim loại. Chúng tôi mô tả thiết kế, điều khiển, đặc điểm và giới hạn của thiết bị. Hơn nữa, chúng tôi cung cấp các so sánh về hiệu suất neutron của thiết bị của chúng tôi với các tùy chọn có sẵn trên thị trường. Cuối cùng, chúng tôi xem xét các đường nâng cấp để cải thiện hiệu suất và độ tin cậy."} +{"idx": 1920082, "en": "We present an apparatus for performing gas phase high-harmonic generation spectroscopy of molecules primarily found in the liquid phase. Liquid molecular samples are heated in a temperature controlled bath and their vapour is used to back a continuous flow gas jet, with vapour pressures of over 1 bar possible. In order to demonstrate the system, we perform high harmonic spectroscopy experiments in benzene with a 1.8 μm driving field. Using the unique capabilities of the system, we obtain spectra that are nearly free from the effects of longitudinal phase-matching, amenable to comparison with advanced numerical modelling.", "gemini": "Chúng tôi trình bày một thiết bị để thực hiện phổ học tạo sóng hài bậc cao pha khí của các phân tử chủ yếu được tìm thấy trong pha lỏng. Các mẫu phân tử lỏng được đun nóng trong bồn tắm được kiểm soát nhiệt độ và hơi của chúng được sử dụng để tạo ra tia khí dòng liên tục, với áp suất hơi có thể trên 1 bar. Để chứng minh hệ thống, chúng tôi thực hiện các thí nghiệm phổ học sóng hài bậc cao trong benzen với trường điều khiển 1,8 μm. Sử dụng các khả năng độc đáo của hệ thống, chúng tôi thu được các phổ gần như không bị ảnh hưởng của sự ghép pha dọc, có thể so sánh với mô hình số tiên tiến."} +{"idx": 1920083, "en": "A measurement system error is a key factor that disturbs the identification precision of sliding bearing's dynamic characteristic coefficients. The transfer process and influence rule of errors from a measurement system to dynamic characteristic coefficients are analyzed by solving the dynamic characteristic measurement model. In order to ensure that the identification errors are no more than 40%, the amplitude error and phase error of the transfer function of the measurement system should be controlled within 10% and 1°, respectively. A novel magnetic suspension calibration method of the measurement system, which generates a vibration through a noncontact electromagnetic force rather than a traditional contact force, is proposed. A magnetic dynamic calibration device is developed. The experiment results show that the device can make dynamic calibration at different frequencies successfully, which is favorable to improve the controllability of the calibration process and the stability of calibration results.", "gemini": "Lỗi hệ thống đo lường là một yếu tố chính làm nhiễu loạn độ chính xác nhận dạng các hệ số đặc tính động của ổ trượt. Quá trình truyền và quy tắc ảnh hưởng của lỗi từ hệ thống đo lường đến các hệ số đặc tính động được phân tích bằng cách giải mô hình đo đặc tính động. Để đảm bảo rằng lỗi nhận dạng không quá 40%, lỗi biên độ và lỗi pha của hàm truyền của hệ thống đo lường phải được kiểm soát trong phạm vi 10% và 1°. Một phương pháp hiệu chuẩn hệ thống đo lường treo từ trường mới, tạo ra rung động thông qua lực điện từ không tiếp xúc thay vì lực tiếp xúc truyền thống, được đề xuất. Một thiết bị hiệu chuẩn động từ tính được phát triển. Kết quả thí nghiệm cho thấy thiết bị có thể hiệu chuẩn động ở các tần số khác nhau một cách thành công, thuận lợi cho việc cải thiện khả năng kiểm soát quá trình hiệu chuẩn và độ ổn định của kết quả hiệu chuẩn."} +{"idx": 1920084, "en": "In an actual setup of squeezed state generation, the stability of a squeezing factor is mainly limited by the performance of the servo-control system, which is mainly influenced by the shot noise and gain of a photodetector. We present a unique transformer-coupled LC resonant amplifier as a photodetector circuit to reduce the electronic noise and increase the gain of the photodetector. As a result, we obtain a low-noise, high gain photodetector with the gain of more than 1.8×105 V/A, and the input current noise of less than 4.7 pA/Hz. By adjusting the parameters of the transformer, the quality factor Q of the resonant circuit is close to 100 in the frequency range of more than 100 MHz, which meets the requirement for weak power detection in the application of squeezed state generation.", "gemini": "Trong một thiết lập thực tế để tạo ra trạng thái bị ép, độ ổn định của hệ số ép chủ yếu bị giới hạn bởi hiệu suất của hệ thống điều khiển servo, hệ thống này chủ yếu chịu ảnh hưởng của nhiễu xung và độ lợi của một bộ dò quang. Chúng tôi trình bày một bộ khuếch đại cộng hưởng LC ghép máy biến áp độc đáo làm mạch bộ dò quang để giảm nhiễu điện tử và tăng độ lợi của bộ dò quang. Do đó, chúng tôi thu được một bộ dò quang độ lợi cao, nhiễu thấp với độ lợi hơn 1,8×105 V/A và nhiễu dòng đầu vào nhỏ hơn 4,7 pA/Hz. Bằng cách điều chỉnh các thông số của máy biến áp, hệ số品质 Q của mạch cộng hưởng gần bằng 100 trong dải tần số hơn 100 MHz, đáp ứng yêu cầu phát hiện công suất yếu trong ứng dụng tạo ra trạng thái bị ép."} +{"idx": 1920085, "en": "The present design concept for the ITER low-field-side reflectometer transmission line (TL) consists of an ∼40 m long, 6.35 cm diameter helically corrugated waveguide (WG) together with ten 90° miter bends. This paper presents an evaluation of the TL performance at low frequencies (33-50 GHz) where the predicted HE11 mode ohmic and mode conversion losses start to increase significantly. Quasi-optical techniques were used to form a near Gaussian beam to efficiently couple radiation in this frequency range into the WG. It was observed that the output beams from the guide remained linearly polarized with cross-polarization power levels of ∼1.5%-3%. The polarization rotation due to the helical corrugations was in the range ∼1°-3°. The radiated beam power profiles typically show excellent Gaussian propagation characteristics at distances >20 cm from the final exit aperture. The round trip propagation loss was found to be ∼2.5 dB at 50 GHz and ∼6.5 dB at 35 GHz, showing an inverse increase with frequency. This was consistent with updated calculations of miter bend and ohmic losses. At low frequencies (33-50 GHz), the mode purity remained very good at the exit of the waveguide, and the losses are perfectly acceptable for operation in ITER. The primary challenge may come from the future addition of a Gaussian telescope and other filter components within the corrugated guide, which will likely introduce additional perturbations to the beam profile and an increase in mode-conversion loss.", "gemini": "Khái niệm thiết kế hiện tại cho đường truyền phản xạ mặt trường thấp ITER (TL) bao gồm một ống dẫn sóng gợn xoắn ốc có chiều dài ∼40 m, đường kính 6,35 cm cùng với mười khúc uốn 90°. Bài báo này trình bày đánh giá về hiệu suất TL ở tần số thấp (33-50 GHz) trong đó tổn hao chuyển đổi chế độ và điện trở mô HE11 dự đoán bắt đầu tăng đáng kể. Các kỹ thuật quang học bán được sử dụng để tạo chùm tia gần Gauss để ghép bức xạ hiệu quả trong dải tần số này vào WG. Người ta quan sát thấy rằng các chùm tia đầu ra từ hướng dẫn vẫn được phân cực tuyến tính với mức công suất phân cực chéo ∼1,5%-3%. Độ quay phân cực do các gợn xoắn ốc nằm trong khoảng ∼1°-3°. Các cấu hình công suất chùm tia bức xạ thường cho thấy các đặc tính lan truyền Gauss tuyệt vời ở khoảng cách >20 cm so với khẩu độ thoát cuối cùng. Tổn hao lan truyền khứ hồi được phát hiện là ∼2,5 dB ở tần số 50 GHz và ∼6,5 dB ở tần số 35 GHz, cho thấy sự gia tăng nghịch đảo theo tần số. Điều này phù hợp với các tính toán cập nhật về tổn hao uốn khúc và điện trở. Ở tần số thấp (33-50 GHz), độ tinh khiết của chế độ vẫn rất tốt ở đầu ra của ống dẫn sóng và tổn hao hoàn toàn có thể chấp nhận được để vận hành trong ITER. Thách thức chính có thể đến từ việc bổ sung kính thiên văn Gauss và các thành phần bộ lọc khác trong tương lai bên trong hướng dẫn gợn sóng, điều này có thể sẽ gây ra nhiễu loạn bổ sung cho cấu hình chùm tia và làm tăng tổn hao chuyển đổi chế độ."} +{"idx": 1920086, "en": "A stable femtosecond electron beam system has been configured for time-resolved pump-probe experiments. The ultrafast electron diffraction (UED) system is designed with a sub-MeV photoelectron beam source pulsed by a femtosecond UV laser and nondispersive beamline components, including a bunch compressor-a pulsed S-band klystron is installed and fully commissioned with 5.5 MW peak power in a 2.5 μs pulse length. A single-cell RF photo-gun is designed to produce 1.6-16 pC electron bunches in a photoemission mode with 150 fs pulse duration at 0.5-1 MeV. The measured RF system jitters are within 1% in magnitude and 0.2° in phase, which would induce 3.4 keV and 0.25 keV of ΔE, corresponding to 80 fs and 5 fs of Δt, respectively. Our particle-in-cell simulations indicate that the designed bunch compressor reduces the time-of-arrival jitter by about an order of magnitude. The transport and focusing optics of the designed beamline with the bunch compressor enables an energy spread within 10-4 and a bunch length (electron probe) within <500 fs. In this paper, the design analysis and instrumental test results are presented along with the development of the quasi-relativistic UED system.", "gemini": "Một hệ thống chùm tia điện tử femtosecond ổn định đã được cấu hình cho các thí nghiệm bơm-dò được giải quyết theo thời gian. Hệ thống nhiễu xạ điện tử cực nhanh (UED) được thiết kế với nguồn chùm tia quang điện tử dưới MeV được xung bằng tia laser UV femtosecond và các thành phần đường chùm không phân tán, bao gồm một bộ nén chùm tia - một klystron S-band xung được lắp đặt và đưa vào hoạt động hoàn toàn với công suất đỉnh 5,5 MW trong độ dài xung 2,5 μs. Một súng quang RF một ô được thiết kế để tạo ra các chùm electron 1,6-16 pC ở chế độ quang phát xạ với độ dài xung 150 fs ở 0,5-1 MeV. Độ nhiễu của hệ thống RF được đo lường nằm trong phạm vi 1% về độ lớn và 0,2° về pha, điều này sẽ gây ra ΔE là 3,4 keV và 0,25 keV, tương ứng với Δt là 80 fs và 5 fs. Các mô phỏng hạt trong ô của chúng tôi chỉ ra rằng bộ nén chùm tia được thiết kế làm giảm độ nhiễu thời gian đến khoảng một bậc độ lớn. Quang học vận chuyển và hội tụ của đường chùm được thiết kế với bộ nén chùm tia cho phép độ rộng năng lượng trong vòng 10-4 và độ dài chùm tia (điện tử dò) trong vòng <500 fs. Trong bài báo này, kết quả phân tích thiết kế và thử nghiệm dụng cụ được trình bày cùng với sự phát triển của hệ thống UED tương đối tính."} +{"idx": 1920087, "en": "With the development of deep learning, research on image target recognition has made great progress in recent years. Remote sensing detection urgently requires target recognition for military, geographic, and other scientific research. This paper aims to solve the synthetic aperture radar image target recognition problem by combining deep and kernel learning. The model, which has a multilayer multiple kernel structure, is optimized layer by layer with the parameters of Support Vector Machine and a gradient descent algorithm. This new deep kernel learning method improves accuracy and achieves competitive recognition results compared with other learning methods.", "gemini": "Với sự phát triển của học sâu, nghiên cứu về nhận dạng mục tiêu hình ảnh đã đạt được tiến bộ vượt bậc trong những năm gần đây. Phát hiện từ xa đòi hỏi phải nhận dạng mục tiêu cho các nghiên cứu khoa học quân sự, địa lý và các nghiên cứu khoa học khác. Bài báo này nhằm giải quyết vấn đề nhận dạng mục tiêu hình ảnh radar khẩu độ tổng hợp bằng cách kết hợp học sâu và học hạt nhân. Mô hình có cấu trúc hạt nhân nhiều lớp được tối ưu hóa từng lớp với các tham số của Máy vectơ hỗ trợ và thuật toán giảm độ dốc. Phương pháp học hạt nhân sâu mới này cải thiện độ chính xác và đạt được kết quả nhận dạng cạnh tranh so với các phương pháp học khác."} +{"idx": 1920088, "en": "Time-domain interferometry (TDI) based on nuclear resonant scattering of synchrotron radiation by Mössbauer nuclei is a promising technique to study slow dynamics at the interatomic length scale. In order to improve the efficiency of this technique, a new TDI scheme is developed involving the use of a nuclear absorber with a two-line energy spectrum combined with a single-line spectrum. Different from other TDI setups, the issue of external vibrations is much reduced since the two absorbers are at rest and no velocity transducer is used. This allows measuring beating patterns with satisfying statistical accuracy and contrast up to 350 ns. We report here the characterization of the experimental setup necessary for the implementation of this new scheme. The model required for the description of the beating pattern produced by a three-line spectrum system is also discussed in detail. Finally, we report some results for the dynamics of the prototypical glass-former ortho-terphenyl to demonstrate the possibilities offered by this new scheme.", "gemini": "Giao thoa miền thời gian (TDI) dựa trên sự tán xạ cộng hưởng hạt nhân của bức xạ synchrotron bởi các hạt nhân Mössbauer là một kỹ thuật đầy hứa hẹn để nghiên cứu động lực học chậm ở thang độ dài liên nguyên tử. Để cải thiện hiệu quả của kỹ thuật này, một lược đồ TDI mới được phát triển liên quan đến việc sử dụng bộ hấp thụ hạt nhân với phổ năng lượng hai dòng kết hợp với phổ một dòng. Khác với các thiết lập TDI khác, vấn đề rung động bên ngoài giảm đi nhiều vì hai bộ hấp thụ ở trạng thái nghỉ và không sử dụng bộ chuyển đổi tốc độ. Điều này cho phép đo các mẫu nhịp với độ chính xác thống kê thỏa đáng và độ tương phản lên đến 350 ns. Chúng tôi báo cáo ở đây đặc điểm của thiết lập thử nghiệm cần thiết để thực hiện lược đồ mới này. Mô hình cần thiết để mô tả mẫu nhịp tạo ra bởi hệ thống phổ ba dòng cũng được thảo luận chi tiết. Cuối cùng, chúng tôi báo cáo một số kết quả về động lực học của chất tạo thành thủy tinh nguyên mẫu ortho-terphenyl để chứng minh các khả năng mà lược đồ mới này mang lại."} +{"idx": 1920089, "en": "An x-ray spectrometer employing a thin (50 μm) silicon transmission crystal was used to record high-resolution Cu Kα spectra from a laboratory x-ray source. The diffraction was from the (331) planes that were at an angle of 13.26° to the crystal surface. The components of the spectral lines resulting from single-vacancy (1s) and double-vacancy (1s and 3d) transitions were observed. After accounting for the natural lifetime widths from reference double-crystal spectra and the spatial resolution of the image plate detector, the intrinsic broadening of the transmission crystal was measured to be as small as 0.67 eV and the resolving power 12 000, the highest resolving power achieved by a compact (0.5 m long) spectrometer employing a single transmission crystal operating in the hard x-ray region. By recording spectra with variable source-to-crystal distances and comparing to the calculated widths from various geometrical broadening mechanisms, the primary contributions to the intrinsic crystal broadening were found to be the source height at small distances and the crystal apertured height at large distances. By reducing these two effects, using a smaller source size and vignetting the crystal height, the intrinsic crystal broadening is then limited by the crystal thickness and the rocking curve width and would be 0.4 eV at 8 keV energy (20 000 resolving power).", "gemini": "Một máy quang phổ tia X sử dụng tinh thể truyền silicon mỏng (50 μm) đã được sử dụng để ghi lại phổ Cu Kα có độ phân giải cao từ nguồn tia X trong phòng thí nghiệm. Sự nhiễu xạ là từ các mặt phẳng (331) tạo thành góc 13,26° so với bề mặt tinh thể. Các thành phần của các vạch phổ phát sinh từ các chuyển tiếp khuyết đơn (1s) và khuyết đôi (1s và 3d) đã được quan sát. Sau khi tính đến độ rộng thời gian sống tự nhiên từ phổ tinh thể đôi tham chiếu và độ phân giải không gian của bộ phát hiện tấm ảnh, độ rộng bên trong của tinh thể truyền được đo là nhỏ tới 0,67 eV và độ phân giải là 12.000, độ phân giải cao nhất đạt được bởi một máy quang phổ nhỏ gọn (dài 0,5 m) sử dụng một tinh thể truyền duy nhất hoạt động trong vùng tia X cứng. Bằng cách ghi lại phổ với khoảng cách nguồn đến tinh thể thay đổi và so sánh với độ rộng được tính toán từ các cơ chế mở rộng hình học khác nhau, người ta thấy rằng những đóng góp chính cho độ mở rộng tinh thể bên trong là chiều cao nguồn ở khoảng cách nhỏ và chiều cao khẩu độ tinh thể ở khoảng cách lớn. Bằng cách giảm hai hiệu ứng này, sử dụng kích thước nguồn nhỏ hơn và làm tối ưu chiều cao tinh thể, độ mở rộng tinh thể bên trong sau đó bị giới hạn bởi độ dày tinh thể và độ rộng đường cong lắc và sẽ là 0,4 eV ở năng lượng 8 keV (độ phân giải 20.000)."} +{"idx": 1920090, "en": "Evidence has been accumulating that speakers adjust their speech production to account for conditions of signal transmission to an interlocutor, in addition to speaker- and listener-related influences. This study manipulated acoustic transmission conditions between speakers and a researcher while they completed dyadic cooperative puzzle tasks. Speakers heard evidence of four transmission perturbations (intensity increase/decrease, pitch increase/decrease) only indirectly, i.e., via changes in the researcher's voice. Analysis of speakers' conversational turns across conditions (baseline + four partner-voice manipulations) showed significant group-level imitation of transmission manipulations of partner voice intensity, with no general response to shifts in partner voice pitch. Significant voice alterations were also observed for individual speakers in intensity, with substantial inter-talker variability in responses. Thirteen of 29 speakers exclusively imitated the manipulation of partner intensity, 4/29 speakers exclusively opposed the transmission manipulation, and 4/29 speakers showed both response types. The presence of opposition responses coupled with a relatively low-magnitude group-level convergence suggests the possibility of a compensatory force at work in at least some speakers, adjusting for changes made during signal transmission despite their lack of direct experience with the transmission manipulation effects.", "gemini": "Bằng chứng đã tích lũy cho thấy người nói điều chỉnh cách phát âm của họ để tính đến các điều kiện truyền tín hiệu đến người đối thoại, ngoài các ảnh hưởng liên quan đến người nói và người nghe. Nghiên cứu này đã điều chỉnh các điều kiện truyền âm thanh giữa người nói và nhà nghiên cứu trong khi họ hoàn thành các nhiệm vụ giải đố hợp tác theo cặp. Người nói nghe thấy bằng chứng về bốn nhiễu loạn truyền dẫn (tăng / giảm cường độ, tăng / giảm độ cao) chỉ gián tiếp, tức là thông qua những thay đổi trong giọng nói của nhà nghiên cứu. Phân tích các lượt hội thoại của người nói trên các điều kiện (điểm chuẩn + bốn thao tác giọng nói của đối tác) cho thấy sự bắt chước đáng kể ở cấp độ nhóm đối với các thao tác truyền dẫn cường độ giọng nói của đối tác, không có phản ứng chung đối với sự thay đổi cao độ giọng nói của đối tác. Những thay đổi giọng nói đáng kể cũng được quan sát thấy ở những người nói riêng lẻ về cường độ, với sự thay đổi đáng kể giữa các người nói trong các phản ứng. Mười ba trong số 29 người nói chỉ bắt chước thao tác cường độ của đối tác, 4/29 người nói chỉ phản đối thao tác truyền dẫn và 4/29 người nói thể hiện cả hai loại phản ứng. Sự hiện diện của các phản ứng đối lập cùng với sự hội tụ ở cấp độ nhóm có độ lớn tương đối thấp cho thấy khả năng có một lực bù trừ đang hoạt động ở ít nhất một số người nói, điều chỉnh các thay đổi được thực hiện trong quá trình truyền tín hiệu mặc dù họ không có kinh nghiệm trực tiếp với các hiệu ứng thao tác truyền tín hiệu."} +{"idx": 1920091, "en": "This report is an addendum to a previous report by Burns [(2009). J. Acoust. Soc. Am. 125, 3166-3176] that measured spontaneous otoacoustic emissions (SOAEs) in 18 subjects, whose ages at the time of initial measurement ranged from 6 to 42 yr, for follow-up periods of up to 19.5 yr. The major finding of that report was that the frequencies of all SOAEs, in all subjects, declined over time, with an average decline of 0.25% per year. In this report seven SOAEs in the oldest subject were measured for an additional 13.7 yr, for a total follow-up of 33 yr, to age 75.", "gemini": "Báo cáo này là phần phụ lục cho báo cáo trước của Burns [(2009). J. Acoust. Soc. Am. 125, 3166-3176] đã đo phát xạ âm thanh tự phát (SOAE) ở 18 đối tượng, độ tuổi khi đo ban đầu từ 6 đến 42 tuổi, trong các giai đoạn theo dõi lên đến 19,5 năm. Phát hiện chính của báo cáo đó là tần số của tất cả các SOAE, ở tất cả các đối tượng, đều giảm theo thời gian, với mức giảm trung bình 0,25% mỗi năm. Trong báo cáo này, bảy SOAE ở đối tượng lớn tuổi nhất đã được đo trong thêm 13,7 năm, tổng thời gian theo dõi là 33 năm, đến 75 tuổi."} +{"idx": 1920092, "en": "Vowels are complex sounds with four to five spectral peaks known as formants. The frequencies of the two lowest formants, F1and F2, are sufficient for vowel discrimination. Behavioral studies show that many birds and mammals can discriminate vowels. However, few studies have quantified thresholds for formant-frequency discrimination. The present study examined formant-frequency discrimination in budgerigars (Melopsittacus undulatus) and humans using stimuli with one or two formants and a constant fundamental frequency of 200 Hz. Stimuli had spectral envelopes similar to natural speech and were presented with random level variation. Thresholds were estimated for frequency discrimination of F1, F2, and simultaneous F1 and F2 changes. The same two-down, one-up tracking procedure and single-interval, two-alternative task were used for both species. Formant-frequency discrimination thresholds were as sensitive in budgerigars as in humans and followed the same patterns across all conditions. Thresholds expressed as percent frequency difference were higher for F1 than for F2, and were unchanged between stimuli with one or two formants. Thresholds for simultaneous F1 and F2 changes indicated that discrimination was based on combined information from both formant regions. Results were consistent with previous human studies and show that budgerigars provide an exceptionally sensitive animal model of vowel feature discrimination.", "gemini": "Nguyên âm là những âm thanh phức tạp với bốn đến năm đỉnh phổ được gọi là formant. Tần số của hai formant thấp nhất, F1 và F2, đủ để phân biệt nguyên âm. Các nghiên cứu về hành vi cho thấy nhiều loài chim và động vật có vú có thể phân biệt các nguyên âm. Tuy nhiên, rất ít nghiên cứu định lượng ngưỡng phân biệt tần số formant. Nghiên cứu hiện tại đã kiểm tra sự phân biệt tần số formant ở vẹt (Melopsittacus undulatus) và con người bằng cách sử dụng các kích thích có một hoặc hai formant và tần số cơ bản không đổi là 200 Hz. Các kích thích có bao phổ tương tự như lời nói tự nhiên và được trình bày với sự thay đổi mức độ ngẫu nhiên. Ngưỡng được ước tính cho sự phân biệt tần số của F1, F2 và các thay đổi F1 và F2 đồng thời. Cùng một quy trình theo dõi hai xuống, một lên và nhiệm vụ hai lựa chọn thay thế, một khoảng thời gian đã được sử dụng cho cả hai loài. Ngưỡng phân biệt tần số formant nhạy ở vẹt như ở người và tuân theo cùng một kiểu trên tất cả các điều kiện. Ngưỡng được biểu thị dưới dạng phần trăm chênh lệch tần số cao hơn đối với F1 so với F2 và không thay đổi giữa các kích thích có một hoặc hai formant. Ngưỡng cho các thay đổi F1 và F2 đồng thời chỉ ra rằng sự phân biệt dựa trên thông tin kết hợp từ cả hai vùng formant. Kết quả phù hợp với các nghiên cứu trước đây trên người và cho thấy rằng vẹt cung cấp một mô hình động vật đặc biệt nhạy cảm để phân biệt các đặc điểm của nguyên âm."} +{"idx": 1920093, "en": "Ambient noise in the eastern Arctic was studied from April to September 2013 using a 22 element vertical hydrophone array as it drifted from near the North Pole (89° 23'N, 62° 35'W) to north of Fram Strait (83° 45'N, 4° 28'W). The hydrophones recorded for 108 min/day on six days per week with a sampling rate of 1953.125 Hz. After removal of data corrupted by non-acoustic transients, 19 days throughout the transit period were analyzed. Noise contributors identified include broadband and tonal ice noises, bowhead whale calling, seismic airgun surveys, and earthquake T phases. The bowhead whale or whales detected are believed to belong to the endangered Spitsbergen population, and were recorded when the array was as far north as 86° 24'N. Median power spectral estimates and empirical probability density functions along the array transit show a change in the ambient noise levels corresponding to seismic survey airgun occurrence and received level at low frequencies and transient ice noises at high frequencies. Median power for the same periods across the array shows that this change is consistent in depth. The median ambient noise for May 2013 was among the lowest of the sparse reported observations in the eastern Arctic but comparable to the more numerous observations of western Arctic noise levels.", "gemini": "Tiếng ồn xung quanh ở Bắc Cực phía đông đã được nghiên cứu từ tháng 4 đến tháng 9 năm 2013 bằng cách sử dụng một mảng thủy âm thẳng đứng 22 phần tử khi nó trôi từ gần Cực Bắc (89° 23'N, 62° 35'W) đến phía bắc eo biển Fram (83° 45'N, 4° 28'W). Các thủy âm đã ghi lại trong 108 phút/ngày trong sáu ngày mỗi tuần với tốc độ lấy mẫu là 1953,125 Hz. Sau khi loại bỏ dữ liệu bị hỏng do các xung động không phải âm thanh, 19 ngày trong suốt thời gian vận chuyển đã được phân tích. Các tác nhân gây tiếng ồn được xác định bao gồm tiếng ồn băng thông rộng và âm sắc, tiếng gọi của cá voi đầu cong, các cuộc khảo sát bằng súng hơi địa chấn và pha T động đất. Người ta tin rằng cá voi đầu cong hoặc cá voi được phát hiện thuộc về quần thể Spitsbergen đang bị đe dọa và được ghi lại khi mảng này ở xa về phía bắc đến 86° 24'N. Các ước tính phổ công suất trung bình và các hàm mật độ xác suất kinh nghiệm dọc theo quá trình vận chuyển mảng cho thấy sự thay đổi về mức độ tiếng ồn xung quanh tương ứng với sự xuất hiện của súng hơi khảo sát địa chấn và mức độ nhận được ở tần số thấp và tiếng ồn băng tạm thời ở tần số cao. Công suất trung bình cho cùng các khoảng thời gian trên toàn bộ mảng cho thấy sự thay đổi này là đồng nhất theo độ sâu. Tiếng ồn xung quanh trung bình trong tháng 5 năm 2013 nằm trong số những quan sát được báo cáo ít nhất ở Bắc Cực phía đông nhưng tương đương với nhiều quan sát hơn về mức độ tiếng ồn ở Bắc Cực phía tây."} +{"idx": 1920094, "en": "A unified framework is proposed for analysis and synthesis of two-dimensional spatial sound field in reverberant environments. In the sound field analysis (SFA) phase, an unbaffled 24-element circular microphone array is utilized to encode the sound field based on the plane-wave decomposition. Depending on the sparsity of the sound sources, the SFA stage can be implemented in two manners. For sparse-source scenarios, a one-stage algorithm based on compressive sensing algorithm is utilized. Alternatively, a two-stage algorithm can be used, where the minimum power distortionless response beamformer is used to localize the sources and Tikhonov regularization algorithm is used to extract the source amplitudes. In the sound field synthesis (SFS), a 32-element rectangular loudspeaker array is employed to decode the target sound field using pressure matching technique. To establish the room response model, as required in the pressure matching step of the SFS phase, an SFA technique for nonsparse-source scenarios is utilized. Choice of regularization parameters is vital to the reproduced sound field. In the SFS phase, three SFS approaches are compared in terms of localization performance and voice reproduction quality. Experimental results obtained in a reverberant room are presented and reveal that an accurate room response model is vital to immersive rendering of the reproduced sound field.", "gemini": "Một khuôn khổ thống nhất được đề xuất để phân tích và tổng hợp trường âm thanh không gian hai chiều trong môi trường có tiếng vang. Trong giai đoạn phân tích trường âm (SFA), một mảng micrô tròn 24 phần tử không có vành được sử dụng để mã hóa trường âm dựa trên phân rã sóng phẳng. Tùy thuộc vào độ thưa thớt của các nguồn âm thanh, giai đoạn SFA có thể được thực hiện theo hai cách. Đối với các tình huống nguồn thưa thớt, một thuật toán một giai đoạn dựa trên thuật toán cảm biến nén được sử dụng. Ngoài ra, có thể sử dụng một thuật toán hai giai đoạn, trong đó bộ tạo chùm đáp ứng không gây méo công suất tối thiểu được sử dụng để định vị các nguồn và thuật toán điều chỉnh Tikhonov được sử dụng để trích xuất biên độ nguồn. Trong tổng hợp trường âm (SFS), một mảng loa hình chữ nhật 32 phần tử được sử dụng để giải mã trường âm mục tiêu bằng kỹ thuật khớp áp suất. Để thiết lập mô hình đáp ứng của phòng, theo yêu cầu trong bước khớp áp suất của giai đoạn SFS, một kỹ thuật SFA cho các tình huống nguồn không thưa thớt được sử dụng. Việc lựa chọn các tham số điều chỉnh là rất quan trọng đối với trường âm được tái tạo. Trong giai đoạn SFS, ba phương pháp SFS được so sánh về hiệu suất định vị và chất lượng tái tạo giọng nói. Các kết quả thực nghiệm thu được trong một căn phòng có tiếng vang được trình bày và cho thấy rằng một mô hình đáp ứng phòng chính xác là rất quan trọng đối với việc kết xuất nhập vai của trường âm được tái tạo."} +{"idx": 1920095, "en": "Ultrasound signals that pass through cancellous bone may be considered to consist of two longitudinal waves, which are called fast and slow waves. Accurate decomposition of these fast and slow waves is considered to be highly beneficial in determination of the characteristics of cancellous bone. In the present study, a fast decomposition method using a wave transfer function with a phase rotation parameter was applied to received signals that have passed through bovine bone specimens with various bone volume to total volume (BV/TV) ratios in a simulation study, where the elastic finite-difference time-domain method is used and the ultrasound wave propagated parallel to the bone axes. The proposed method succeeded to decompose both fast and slow waves accurately; the normalized residual intensity was less than -19.5 dB when the specimen thickness ranged from 4 to 7 mm and the BV/TV value ranged from 0.144 to 0.226. There was a strong relationship between the phase rotation value and the BV/TV value. The ratio of the peak envelope amplitude of the decomposed fast wave to that of the slow wave increased monotonically with increasing BV/TV ratio, indicating the high performance of the proposed method in estimation of the BV/TV value in cancellous bone.", "gemini": "Các tín hiệu siêu âm đi qua xương xốp có thể được coi là bao gồm hai sóng dọc, được gọi là sóng nhanh và sóng chậm. Phân rã chính xác các sóng nhanh và chậm này được coi là rất có lợi trong việc xác định các đặc điểm của xương xốp. Trong nghiên cứu hiện tại, một phương pháp phân rã nhanh sử dụng hàm truyền sóng với tham số quay pha đã được áp dụng cho các tín hiệu thu được đã đi qua các mẫu xương bò với tỷ lệ thể tích xương trên tổng thể tích (BV/TV) khác nhau trong một nghiên cứu mô phỏng, trong đó sử dụng phương pháp miền thời gian hữu hạn đàn hồi và sóng siêu âm lan truyền song song với trục xương. Phương pháp được đề xuất đã phân rã thành công cả sóng nhanh và sóng chậm một cách chính xác; cường độ dư chuẩn hóa nhỏ hơn -19,5 dB khi độ dày mẫu từ 4 đến 7 mm và giá trị BV/TV từ 0,144 đến 0,226. Có mối quan hệ chặt chẽ giữa giá trị quay pha và giá trị BV/TV. Tỷ lệ biên độ đỉnh của sóng nhanh phân rã so với sóng chậm tăng đơn điệu khi tỷ lệ BV/TV tăng, cho thấy hiệu suất cao của phương pháp được đề xuất trong việc ước tính giá trị BV/TV trong xương xốp."} +{"idx": 1920096, "en": "Safety criteria for naval sonar sounds are needed to protect harbor porpoise hearing. Two porpoises were exposed to sequences of AN/SQS-53C sonar playback sounds (3.5-4.1 kHz, without significant harmonics), at a mean received sound pressure level of 142 dB re 1 μPa, with a duty cycle of 96% (almost continuous). Behavioral hearing thresholds at 4 and 5.7 kHz were determined before and after exposure to the fatiguing sound, in order to quantify temporary threshold shifts (TTSs) and hearing recovery. Control sessions were also conducted. Significant mean initial TTS1-4 of 5.2 dB at 4 kHz and 3.1 dB at 5.7 kHz occurred after 30 min exposures (mean received cumulative sound exposure level, SELcum: 175 dB re 1 μPa2s). Hearing thresholds returned to pre-exposure levels within 12 min. Significant mean initial TTS1-4 of 5.5 dB at 4 kHz occurred after 60 min exposures (SELcum: 178 dB re 1 μPa2s). Hearing recovered within 60 min. The SELcum for AN/SQS-53C sonar sounds required to induce 6 dB of TTS 4 min after exposure (the definition of TTS onset) is expected to be between 175 and 180 dB re 1 μPa2s.", "gemini": "Cần có tiêu chuẩn an toàn cho âm thanh sonar của tàu hải quân để bảo vệ thính giác của cá cảng. Hai con cá cảng đã tiếp xúc với các chuỗi âm thanh phát lại sonar AN/SQS-53C (3,5-4,1 kHz, không có sóng hài đáng kể), ở mức áp suất âm thanh trung bình nhận được là 142 dB re 1 μPa, với chu kỳ làm việc là 96% (gần như liên tục). Ngưỡng nghe hành vi ở 4 và 5,7 kHz đã được xác định trước và sau khi tiếp xúc với âm thanh gây mệt mỏi, để định lượng sự dịch chuyển ngưỡng tạm thời (TTS) và phục hồi thính giác. Các phiên điều khiển cũng được tiến hành. TTS1-4 ban đầu trung bình đáng kể là 5,2 dB ở 4 kHz và 3,1 dB ở 5,7 kHz xảy ra sau 30 phút tiếp xúc (mức phơi nhiễm âm thanh tích lũy trung bình nhận được, SELcum: 175 dB re 1 μPa2s). Ngưỡng nghe trở lại mức trước khi tiếp xúc trong vòng 12 phút. TTS1-4 ban đầu trung bình đáng kể là 5,5 dB ở 4 kHz xảy ra sau 60 phút tiếp xúc (SELcum: 178 dB re 1 μPa2s). Thính giác phục hồi trong vòng 60 phút. SELcum cho âm thanh sonar AN/SQS-53C cần thiết để gây ra TTS 6 dB sau 4 phút tiếp xúc (định nghĩa về khởi phát TTS) dự kiến nằm trong khoảng từ 175 đến 180 dB re 1 μPa2s."} +{"idx": 1920097, "en": "The existing exposure-response relationships describing the association between wind turbine sound level and noise annoyance concern turbine sizes of 0.15-3.0 MW. The main purpose of this study was to determine a relationship concerning turbines with nominal power of 3-5 MW. A cross-sectional survey was conducted around three wind power areas in Finland. The survey involved all households within a 2 km distance from the nearest turbine. Altogether, 429 households out of 753 participated. The households were exposed to wind turbine noise having sound levels within 26.7-44.2 dB LAeq. Standard prediction methods were applied to determine the sound level, LAeq, in each participant's yard. The measured sound level agreed well with the predicted sound level. The exposure-response relationship was derived between LAeq outdoors and the indoor noise annoyance. The relationship was in rather good agreement with two previous studies involving much smaller turbines (0.15-1.5 MW) under 40 dB LAeq. The Community Tolerance Level (CTL), CTL20 = 50 dB, was 3 dB lower than for two previous studies. Above 40 dB, a small number of participants prevented a reliable comparison to previous studies.", "gemini": "Các mối quan hệ giữa phơi nhiễm và đáp ứng hiện có mô tả mối liên hệ giữa mức âm thanh của tua-bin gió và sự khó chịu do tiếng ồn liên quan đến kích thước tua-bin từ 0,15-3,0 MW. Mục đích chính của nghiên cứu này là xác định mối quan hệ liên quan đến các tua-bin có công suất danh nghĩa từ 3-5 MW. Một cuộc khảo sát cắt ngang đã được tiến hành xung quanh ba khu vực điện gió ở Phần Lan. Cuộc khảo sát bao gồm tất cả các hộ gia đình trong phạm vi 2 km tính từ tua-bin gần nhất. Tổng cộng có 429 hộ gia đình trong số 753 hộ gia đình tham gia. Các hộ gia đình tiếp xúc với tiếng ồn của tua-bin gió có mức âm thanh trong khoảng 26,7-44,2 dB LAeq. Các phương pháp dự đoán tiêu chuẩn đã được áp dụng để xác định mức âm thanh, LAeq, trong sân của mỗi người tham gia. Mức âm thanh đo được phù hợp với mức âm thanh dự đoán. Mối quan hệ giữa phơi nhiễm và đáp ứng được bắt nguồn từ LAeq ngoài trời và sự khó chịu do tiếng ồn trong nhà. Mối quan hệ này khá phù hợp với hai nghiên cứu trước đó liên quan đến các tua-bin nhỏ hơn nhiều (0,15-1,5 MW) dưới 40 dB LAeq. Mức dung nạp của cộng đồng (CTL), CTL20 = 50 dB, thấp hơn 3 dB so với hai nghiên cứu trước đó. Trên 40 dB, một số ít người tham gia đã ngăn cản việc so sánh đáng tin cậy với các nghiên cứu trước đây."} +{"idx": 1920098, "en": "The perception of stress is highly influenced by listeners' native language. In this research, the authors examined the effect of intonation and talker variability (here: phonetic variability) in the discrimination of Spanish lexical stress contrasts by native Spanish (N = 17), German (N = 21), and French (N = 27) listeners. Participants listened to 216 trials containing three Spanish disyllabic words, where one word carried a different lexical stress to the others. The listeners' task was to identify the deviant word in each trial (Odd-One-Out task). The words in the trials were produced by either the same talker or by two different talkers, and carried the same or varying intonation patterns. The German listeners' performance was lower compared to the Spanish listeners but higher than that of the French listeners. French listeners performed above chance level with and without talker variability, and performed at chance level when intonation variability was introduced. Results are discussed in the context of the stress \"deafness\" hypothesis.", "gemini": "Thị giác về căng thẳng chịu ảnh hưởng rất lớn bởi ngôn ngữ bản địa của người nghe. Trong nghiên cứu này, các tác giả đã kiểm tra tác động của ngữ điệu và sự thay đổi của người nói (ở đây: sự thay đổi ngữ âm) trong việc phân biệt sự tương phản về trọng âm từ vựng tiếng Tây Ban Nha của người Tây Ban Nha bản xứ (N = 17), người Đức (N = 21) và người Pháp (N = 27). Người tham gia đã nghe 216 thử nghiệm chứa ba từ tiếng Tây Ban Nha gồm hai âm tiết, trong đó một từ có trọng âm từ vựng khác với các từ còn lại. Nhiệm vụ của người nghe là xác định từ sai lệch trong mỗi thử nghiệm (nhiệm vụ Odd-One-Out). Các từ trong các thử nghiệm được tạo ra bởi cùng một người nói hoặc bởi hai người nói khác nhau và mang các mẫu ngữ điệu giống nhau hoặc khác nhau. Kết quả của người nghe tiếng Đức thấp hơn so với người nghe tiếng Tây Ban Nha nhưng cao hơn so với người nghe tiếng Pháp. Những người nghe tiếng Pháp thực hiện trên mức ngẫu nhiên có và không có sự thay đổi của người nói và thực hiện ở mức ngẫu nhiên khi sự thay đổi ngữ điệu được đưa vào. Kết quả được thảo luận trong bối cảnh của giả thuyết \"điếc\" về căng thẳng."} +{"idx": 1920099, "en": "A high frequency acoustic experiment was conducted in the northern portion of the Gulf of Mexico in August 2016 to examine the operating range, data rates, and performance of acoustic communication systems at the carrier frequency of 85 kHz. The received signal-to-noise ratios, channel coherence, and impulse responses were reported between two 85 kHz transducers and a five-element hydrophone array over multiple ranges up to 1500 m. Channel estimation based decision feedback equalizers (DFEs) were applied to process the communication measurements. A data rate of 27.2 kb/s was achieved for four tested ranges with a uniform set of receiver parameters.", "gemini": "Một thí nghiệm âm thanh tần số cao đã được tiến hành ở phía bắc Vịnh Mexico vào tháng 8 năm 2016 để kiểm tra phạm vi hoạt động, tốc độ dữ liệu và hiệu suất của các hệ thống truyền thông âm thanh ở tần số sóng mang là 85 kHz. Tỷ số tín hiệu trên nhiễu nhận được, độ kết hợp kênh và phản ứng xung đã được báo cáo giữa hai đầu dò 85 kHz và một mảng thủy âm năm phần tử trên nhiều phạm vi lên đến 1500 m. Các bộ cân bằng phản hồi quyết định ước tính kênh (DFE) đã được áp dụng để xử lý các phép đo truyền thông. Tốc độ dữ liệu 27,2 kb/giây đã đạt được cho bốn phạm vi được thử nghiệm với một bộ thông số bộ thu thống nhất."} +{"idx": 1920100, "en": "This study proposes an acoustic theory that describes the resonance phenomena in a resonator made of acoustic composite right/left-handed (CRLH) metamaterials, and verifies it through numerical simulation. The established theory for a microwave CRLH metamaterial resonator is adapted to explain the resonance phenomena in an acoustic CRLH metamaterial resonator. In particular, attention is focused on the zeroth-order resonance phenomenon which has several interesting properties. When a resonator is composed of a CRLH metamaterial, a resonance with a flat acoustic field distribution may occur at one of the frequencies where the wavenumber becomes zero. This resonance is called zeroth-order resonance. Through numerical simulation, such unusual resonance phenomenon in acoustics is observed in more detail and the proposed theory is verified. The results of the theory and the numerical simulation clearly show that zeroth-order resonance can exist at those frequencies where the acoustic field distribution is flat due to infinite wavelength. It is also shown that the resonance frequency and the Q factor of this resonance depend on the boundary condition at both ends of the resonator, and they basically do not change even when the number of units is reduced or increased.", "gemini": "Nghiên cứu này đề xuất một lý thuyết âm học mô tả hiện tượng cộng hưởng trong một bộ cộng hưởng được làm bằng siêu vật liệu thuận/nghịch tay phải (CRLH) âm học và xác minh lý thuyết đó thông qua mô phỏng số. Lý thuyết đã được thiết lập cho một bộ cộng hưởng siêu vật liệu CRLH vi sóng được điều chỉnh để giải thích các hiện tượng cộng hưởng trong một bộ cộng hưởng siêu vật liệu CRLH âm học. Đặc biệt, sự chú ý được tập trung vào hiện tượng cộng hưởng bậc không có một số tính chất thú vị. Khi một bộ cộng hưởng được tạo thành từ siêu vật liệu CRLH, cộng hưởng với phân bố trường âm phẳng có thể xảy ra ở một trong các tần số mà số sóng trở thành không. Cộng hưởng này được gọi là cộng hưởng bậc không. Thông qua mô phỏng số, hiện tượng cộng hưởng bất thường như vậy trong âm học được quan sát chi tiết hơn và lý thuyết được đề xuất được xác minh. Kết quả của lý thuyết và mô phỏng số cho thấy rõ ràng rằng cộng hưởng bậc không có thể tồn tại ở những tần số mà phân bố trường âm là phẳng do bước sóng vô hạn. Người ta cũng chỉ ra rằng tần số cộng hưởng và hệ số Q của cộng hưởng này phụ thuộc vào điều kiện biên ở cả hai đầu của bộ cộng hưởng và về cơ bản chúng không thay đổi ngay cả khi số lượng các đơn vị giảm hoặc tăng."} +{"idx": 1920101, "en": "The frequency bandwidth of the sound from a light helicopter, such as a Robinson R44, extends from about 13 Hz to 2.5 kHz. As such, the R44 has potential as a low-frequency sound source in underwater acoustics applications. To explore this idea, an experiment was conducted in shallow water off the coast of southern California in which a horizontal line of hydrophones detected the sound of an R44 hovering in an end-fire position relative to the array. Some of the helicopter sound interacted with seabed to excite the head wave in the water column. A theoretical analysis of the sound field in the water column generated by a stationary airborne source leads to an expression for the two-point horizontal coherence function of the head wave, which, apart from frequency, depends only on the sensor separation and the sediment sound speed. By matching the zero crossings of the measured and theoretical horizontal coherence functions, the sound speed in the sediment was recovered and found to take a value of 1682.42 ± 16.20 m/s. This is consistent with the sediment type at the experiment site, which is known from a previous survey to be a fine to very-fine sand.", "gemini": "Băng thông tần số của âm thanh từ một máy bay trực thăng hạng nhẹ, chẳng hạn như Robinson R44, kéo dài từ khoảng 13 Hz đến 2,5 kHz. Như vậy, R44 có tiềm năng trở thành nguồn âm tần số thấp trong các ứng dụng âm học dưới nước. Để khám phá ý tưởng này, một thí nghiệm đã được tiến hành ở vùng nước nông ngoài khơi bờ biển Nam California, trong đó một hàng thủy âm nằm ngang phát hiện ra âm thanh của một chiếc R44 lơ lửng ở vị trí cuối cùng so với mảng. Một số âm thanh của máy bay trực thăng tương tác với đáy biển để kích thích sóng đầu trong cột nước. Phân tích lý thuyết về trường âm trong cột nước do nguồn không khí đứng yên tạo ra dẫn đến biểu thức cho hàm kết hợp ngang hai điểm của sóng đầu, ngoài tần số, chỉ phụ thuộc vào khoảng cách cảm biến và tốc độ âm thanh của trầm tích. Bằng cách khớp các điểm không của các hàm kết hợp ngang được đo và lý thuyết, tốc độ âm thanh trong trầm tích đã được phục hồi và có giá trị là 1682,42 ± 16,20 m/giây. Điều này phù hợp với loại trầm tích tại địa điểm thí nghiệm, được biết từ một cuộc khảo sát trước đó là cát mịn đến rất mịn."} +{"idx": 1920102, "en": "Although much is known about the linguistic function of vowel nasality, whether contrastive (as in French) or coarticulatory (as in English), and much effort has gone into identifying potential correlates for the phenomenon, this study examines these proposed features to find the optimal acoustic feature(s) for nasality measurement. To this end, a corpus of 4778 oral and nasal vowels in English and French was collected, and data for 22 features were extracted. A series of linear mixed-effects regressions highlighted three promising features with large oral-to-nasal feature differences and strong effects relative to normal oral vowel variability: A1-P0, F1's bandwidth, and spectral tilt. However, these three features, particularly A1-P0, showed considerable variation in baseline and range across speakers and vowels within each language. Moreover, although the features were consistent in direction across both languages, French speakers' productions showed markedly stronger effects, and showed evidence of spectral tilt beyond the nasal norm being used to enhance the oral-nasal contrast. These findings strongly suggest that the acoustic nature of vowel nasality is both language- and speaker-specific, and that, like vowel formants, nasality measurements require speaker normalization for across-speaker comparison, and that these acoustic properties should not be taken as constant across different languages.", "gemini": "Mặc dù đã biết nhiều về chức năng ngôn ngữ của tính mũi của nguyên âm, dù là tương phản (như trong tiếng Pháp) hay đồng diễn (như trong tiếng Anh) và đã có nhiều nỗ lực trong việc xác định các mối tương quan tiềm ẩn cho hiện tượng này, nghiên cứu này kiểm tra các đặc điểm được đề xuất này để tìm ra các đặc điểm âm thanh tối ưu cho phép đo tính mũi. Để đạt được mục đích này, một tập hợp gồm 4778 nguyên âm miệng và mũi trong tiếng Anh và tiếng Pháp đã được thu thập và dữ liệu cho 22 đặc điểm đã được trích xuất. Một loạt các hồi quy hiệu ứng hỗn hợp tuyến tính đã làm nổi bật ba đặc điểm đầy hứa hẹn với sự khác biệt lớn về đặc điểm từ miệng sang mũi và các hiệu ứng mạnh so với độ biến thiên nguyên âm miệng bình thường: A1-P0, băng thông của F1 và độ nghiêng phổ. Tuy nhiên, ba đặc điểm này, đặc biệt là A1-P0, cho thấy sự khác biệt đáng kể về cơ sở và phạm vi giữa những người nói và nguyên âm trong mỗi ngôn ngữ. Hơn nữa, mặc dù các đặc điểm này nhất quán theo hướng trong cả hai ngôn ngữ, nhưng các sản phẩm của người nói tiếng Pháp cho thấy các hiệu ứng mạnh hơn đáng kể và cho thấy bằng chứng về độ nghiêng phổ vượt quá chuẩn mũi được sử dụng để tăng cường độ tương phản giữa miệng và mũi. Những phát hiện này cho thấy mạnh mẽ rằng bản chất âm thanh của tính mũi của nguyên âm là đặc thù của cả ngôn ngữ và người nói, và giống như các âm vị nguyên âm, phép đo tính mũi đòi hỏi phải chuẩn hóa người nói để so sánh giữa những người nói và các đặc tính âm thanh này không nên được coi là hằng số trong các ngôn ngữ khác nhau."} +{"idx": 1920103, "en": "In recent years, research fields, including ecology, bioacoustics, signal processing, and machine learning, have made bird sound recognition a part of their focus. This has led to significant advancements within the field of ornithology, such as improved understanding of evolution, local biodiversity, mating rituals, and even the implications and realities associated to climate change. The volume of unlabeled bird sound data is now overwhelming, and comparatively little exploration is being made into methods for how best to handle them. In this study, two active learning (AL) methods are proposed, sparse-instance-based active learning (SI-AL), and least-confidence-score-based active learning (LCS-AL), both effectively reducing the need for expert human annotation. To both of these AL paradigms, a kernel-based extreme learning machine (KELM) is then integrated, and a comparison is made to the conventional support vector machine (SVM). Experimental results demonstrate that, when the classifier capacity is improved from an unweighted average recall of 60%-80%, KELM can outperform SVM even when a limited proportion of human annotations are used from the pool of data in both cases of SI-AL (minimum 34.5% vs minimum 59.0%) and LCS-AL (minimum 17.3% vs minimum 28.4%).", "gemini": "Trong những năm gần đây, các lĩnh vực nghiên cứu, bao gồm sinh thái học, âm học sinh học, xử lý tín hiệu và học máy, đã đưa nhận dạng âm thanh của chim vào trọng tâm của họ. Điều này đã dẫn đến những tiến bộ đáng kể trong lĩnh vực nghiên cứu chim, chẳng hạn như cải thiện sự hiểu biết về quá trình tiến hóa, đa dạng sinh học địa phương, nghi thức giao phối và thậm chí cả những tác động và thực tế liên quan đến biến đổi khí hậu. Lượng dữ liệu âm thanh của chim chưa được dán nhãn hiện nay rất lớn và tương đối ít khám phá được thực hiện về các phương pháp xử lý chúng tốt nhất như thế nào. Trong nghiên cứu này, hai phương pháp học tập tích cực (AL) được đề xuất, học tập tích cực dựa trên ví dụ thưa thớt (SI-AL) và học tập tích cực dựa trên điểm số ít tự tin nhất (LCS-AL), cả hai đều có hiệu quả làm giảm nhu cầu chú thích của chuyên gia. Đối với cả hai mô hình AL này, sau đó tích hợp một máy học cực trị dựa trên hạt nhân (KELM) và so sánh với máy vectơ hỗ trợ thông thường (SVM). Kết quả thực nghiệm chứng minh rằng, khi khả năng của bộ phân loại được cải thiện từ độ nhớ lại trung bình không trọng số là 60% -80%, KELM có thể vượt trội hơn SVM ngay cả khi một tỷ lệ chú thích của con người giới hạn được sử dụng từ nhóm dữ liệu trong cả hai trường hợp SI-AL (tối thiểu 34,5% so với tối thiểu 59,0%) và LCS-AL (tối thiểu 17,3% so với tối thiểu 28,4%)."} +{"idx": 1920104, "en": "Based on the assumption that individual sound intensities of singers are incoherent and add linearly to produce a combined choir intensity, a model of a voice range profile of a choir is produced. It is shown that this model predicts six distinct levels of choir dynamics (pp p mp mf f ff) over two octaves of fundamental frequency in a choir section. The levels are 3-6 dB apart, depending on the individual voice range profiles of the singers. Overall choir size has no effect on dynamic range, unless the size is varied dynamically by not all singers singing all the time. For a non-homogeneousn group of singers, a few loud voices dominate ff if everyone sings, while pp is not achieved effectively without suppressing all voices that cannot sing soft. Furthermore, the dynamic range can be significantly limited when choral blend for loudness is imposed on a non-homogeneous choir.", "gemini": "Dựa trên giả định rằng cường độ âm thanh riêng lẻ của các ca sĩ là không mạch lạc và cộng lại theo tuyến tính để tạo ra cường độ hợp xướng kết hợp, một mô hình về biên độ giọng hát của hợp xướng được tạo ra. Người ta thấy rằng mô hình này dự đoán sáu mức động lực hợp xướng riêng biệt (pp p mp mf f ff) trên hai quãng tám của tần số cơ bản trong một phần hợp xướng. Các mức cách nhau 3-6 dB, tùy thuộc vào biên độ giọng hát riêng lẻ của các ca sĩ. Quy mô hợp xướng tổng thể không ảnh hưởng đến dải động, trừ khi quy mô được thay đổi động theo cách không phải tất cả các ca sĩ đều hát mọi lúc. Đối với một nhóm ca sĩ không đồng nhất, một vài giọng hát lớn sẽ chiếm ưu thế trong ff nếu mọi người đều hát, trong khi pp không đạt được hiệu quả nếu không kìm hãm tất cả những giọng hát không thể hát nhẹ. Hơn nữa, dải động có thể bị hạn chế đáng kể khi sự pha trộn hợp xướng để tăng âm lượng được áp đặt lên một hợp xướng không đồng nhất."} +{"idx": 1920105, "en": "There has been little research on the acoustic correlates of emotional expression in the singing voice. In this study, two pertinent questions are addressed: How does a singer's emotional interpretation of a musical piece affect acoustic parameters in the sung vocalizations? Are these patterns specific enough to allow statistical discrimination of the intended expressive targets? Eight professional opera singers were asked to sing the musical scale upwards and downwards (using meaningless content) to express different emotions, as if on stage. The studio recordings were acoustically analyzed with a standard set of parameters. The results show robust vocal signatures for the emotions studied. Overall, there is a major contrast between sadness and tenderness on the one hand, and anger, joy, and pride on the other. This is based on low vs high levels on the components of loudness, vocal dynamics, high perturbation variation, and a tendency for high low-frequency energy. This pattern can be explained by the high power and arousal characteristics of the emotions with high levels on these components. A multiple discriminant analysis yields classification accuracy greatly exceeding chance level, confirming the reliability of the acoustic patterns.", "gemini": "Có rất ít nghiên cứu về mối tương quan giữa âm thanh và biểu hiện cảm xúc trong giọng hát. Trong nghiên cứu này, hai câu hỏi có liên quan được giải quyết: Diễn giải cảm xúc của ca sĩ về một tác phẩm âm nhạc ảnh hưởng như thế nào đến các thông số âm thanh trong giọng hát? Các mẫu này có đủ cụ thể để cho phép phân biệt thống kê các mục tiêu biểu cảm mong muốn không? Tám ca sĩ opera chuyên nghiệp được yêu cầu hát thang âm nhạc lên và xuống (sử dụng nội dung vô nghĩa) để thể hiện những cảm xúc khác nhau, như thể đang trên sân khấu. Các bản ghi âm trong phòng thu đã được phân tích về mặt âm học với một bộ thông số tiêu chuẩn. Kết quả cho thấy chữ ký giọng hát mạnh mẽ cho các cảm xúc được nghiên cứu. Nhìn chung, có sự tương phản lớn giữa nỗi buồn và sự dịu dàng một mặt, và sự tức giận, niềm vui và sự tự hào mặt khác. Điều này dựa trên mức độ thấp so với mức độ cao của các thành phần về độ lớn, động lực giọng nói, độ nhiễu cao và xu hướng năng lượng tần số thấp cao. Mẫu này có thể được giải thích bằng đặc tính công suất và sự kích thích cao của các cảm xúc với mức độ cao trên các thành phần này. Phân tích phân biệt đa biến mang lại độ chính xác phân loại vượt xa mức ngẫu nhiên, xác nhận độ tin cậy của các mẫu âm thanh."} +{"idx": 1920106, "en": "Bats have been observed to shift the frequency of their echolocation calls in the presence of other echolocating bats, ostensibly as a way to reduce acoustic interference. Few studies, however, have examined the theoretical efficacy of such jamming avoidance responses. The present study uses the wideband ambiguity function to analyze the effects of acoustic interference from conspecifics and congeneric heterospecifics on the target acquisition ability of Myotis californicus and Myotis yumanensis, specifically whether unilateral or bilateral frequency shifts reduce the effects of such interference. Model results suggest that in conspecific interactions, M. yumanensis recovers its target acquisition ability more completely and with less absolute frequency shift than does M. californicus, but that alternative methods of jamming avoidance may be easier to implement. The optimal strategy for reducing heterospecific interference is for M. californicus to downshift its call and M. yumanensis to upshift its call, which exaggerates a preexisting difference in mean frequency between the calls of the two species. Further empirical research would elucidate whether these species do in practice actively employ frequency shifting or other means for jamming avoidance, as well as illuminate the role of acoustic interference in niche partitioning.", "gemini": "Người ta đã quan sát thấy rằng loài dơi thay đổi tần số của tiếng gọi định vị bằng tiếng vang khi có sự hiện diện của những con dơi định vị bằng tiếng vang khác, rõ ràng là một cách để giảm nhiễu âm thanh. Tuy nhiên, một số ít nghiên cứu đã kiểm tra hiệu quả lý thuyết của các phản ứng tránh nhiễu như vậy. Nghiên cứu hiện tại sử dụng hàm mơ hồ băng thông rộng để phân tích các tác động của nhiễu âm thanh từ những con dơi cùng loài và những con dơi khác loài cùng họ đối với khả năng tiếp cận mục tiêu của Myotis californicus và Myotis yumanensis, cụ thể là liệu sự dịch chuyển tần số đơn phương hay song phương có làm giảm tác động của nhiễu như vậy hay không. Kết quả mô hình cho thấy rằng trong các tương tác cùng loài, M. yumanensis phục hồi khả năng tiếp cận mục tiêu của mình hoàn toàn hơn và với độ dịch chuyển tần số tuyệt đối ít hơn so với M. californicus, nhưng các phương pháp tránh nhiễu thay thế có thể dễ thực hiện hơn. Chiến lược tối ưu để giảm nhiễu khác loài là M. californicus hạ tần số gọi của mình và M. yumanensis tăng tần số gọi của mình, điều này làm tăng sự khác biệt đã có từ trước về tần số trung bình giữa các tiếng gọi của hai loài. Nghiên cứu thực nghiệm sâu hơn sẽ làm sáng tỏ liệu các loài này có thực sự sử dụng tích cực sự dịch chuyển tần số hay các phương tiện khác để tránh nhiễu hay không, cũng như làm sáng tỏ vai trò của nhiễu âm thanh trong việc phân chia ổ sinh thái."} +{"idx": 1920107, "en": "Inversion methods in shear wave elastography use simplifying assumptions to recover the mechanical properties of soft tissues. Consequently, these methods suffer from artifacts when applied to media containing strong stiffness contrasts, and do not provide a map of the viscosity. In this work, the shear wave field recorded inside and around an inclusion was used to estimate the viscoelastic properties of the inclusion and surrounding medium, based on an inverse problem approach assuming local homogeneity of both media. An efficient semi-analytical method was developed to model the scattering of an elastic wave by an irregular inclusion, based on a decomposition of the field by Bessel functions and on a decomposition of the boundaries as Fourier series. This model was validated against finite element modeling. Shear waves were experimentally induced by acoustic radiation force in soft tissue phantoms containing stiff and soft inclusions, and the displacement field was imaged at a high frame rate using plane wave imaging. A nonlinear least-squares algorithm compared the model to the experimental data and adjusted the geometrical and mechanical parameters. The estimated shear storage and loss moduli were in good agreement with reference measurements, as well as the estimated inclusion shape. This approach provides an accurate estimation of geometry and viscoelastic properties for a single inclusion in a homogeneous background in the context of radiation force elastography.", "gemini": "Các phương pháp đảo ngược trong đàn hồi sóng cắt sử dụng các giả định đơn giản hóa để phục hồi các tính chất cơ học của mô mềm. Do đó, các phương pháp này gặp phải hiện tượng nhiễu khi áp dụng cho các phương tiện có độ cứng tương phản mạnh và không cung cấp bản đồ độ nhớt. Trong công trình này, trường sóng cắt được ghi lại bên trong và xung quanh một tạp chất đã được sử dụng để ước tính các tính chất nhớt đàn hồi của tạp chất và môi trường xung quanh, dựa trên phương pháp tiếp cận bài toán nghịch đảo giả định tính đồng nhất cục bộ của cả hai môi trường. Một phương pháp bán phân tích hiệu quả đã được phát triển để mô hình hóa sự tán xạ của sóng đàn hồi bởi một tạp chất bất thường, dựa trên sự phân hủy trường bằng các hàm Bessel và sự phân hủy các ranh giới thành chuỗi Fourier. Mô hình này đã được xác nhận so với mô hình phần tử hữu hạn. Các sóng cắt được tạo ra bằng lực bức xạ âm thanh trong các mô hình mô mềm có chứa các tạp chất cứng và mềm, và trường dịch chuyển được chụp ở tốc độ khung hình cao bằng hình ảnh sóng phẳng. Một thuật toán bình phương tối thiểu phi tuyến tính so sánh mô hình với dữ liệu thực nghiệm và điều chỉnh các thông số hình học và cơ học. Các mô đun lưu trữ và mất cắt ước tính phù hợp với các phép đo tham chiếu, cũng như hình dạng tạp chất ước tính. Phương pháp tiếp cận này cung cấp ước tính chính xác về hình dạng và tính chất nhớt đàn hồi cho một tạp chất duy nhất trong nền đồng nhất trong bối cảnh đàn hồi lực bức xạ."} +{"idx": 1920108, "en": "This pilot study used acoustic speech analysis to monitor patients with heart failure (HF), which is characterized by increased intracardiac filling pressures and peripheral edema. HF-related edema in the vocal folds and lungs is hypothesized to affect phonation and speech respiration. Acoustic measures of vocal perturbation and speech breathing characteristics were computed from sustained vowels and speech passages recorded daily from ten patients with HF undergoing inpatient diuretic treatment. After treatment, patients displayed a higher proportion of automatically identified creaky voice, increased fundamental frequency, and decreased cepstral peak prominence variation, suggesting that speech biomarkers can be early indicators of HF.", "gemini": "Nghiên cứu thí điểm này sử dụng phân tích giọng nói âm học để theo dõi những bệnh nhân bị suy tim (HF), đặc trưng bởi tăng áp lực làm đầy trong tim và phù ngoại biên. Phù liên quan đến HF ở dây thanh và phổi được đưa ra giả thuyết là ảnh hưởng đến phát âm và hô hấp khi nói. Các biện pháp âm học về nhiễu loạn giọng nói và đặc điểm hô hấp khi nói đã được tính toán từ các nguyên âm kéo dài và các đoạn nói được ghi lại hàng ngày từ mười bệnh nhân bị HF đang được điều trị lợi tiểu nội trú. Sau khi điều trị, bệnh nhân biểu hiện tỷ lệ giọng nói khàn được xác định tự động cao hơn, tần số cơ bản tăng lên và độ biến thiên nổi bật của đỉnh phổ giảm, cho thấy các dấu ấn sinh học về giọng nói có thể là các chỉ số sớm của HF."} +{"idx": 1920109, "en": "A spherical microphone array has proved effective in reconstructing an enclosed sound field by a superposition of spherical wave functions in Fourier domain. It allows successful reconstructions surrounding the array, but the accuracy will be degraded at a distance. In order to extend the effective reconstruction to the entire cavity, a plane-wave basis in space domain is used owing to its non-decaying propagating characteristic and compared with the conventional spherical wave function method in a low frequency sound field within a cylindrical cavity. The sensitivity to measurement noise, the effects of the numbers of plane waves, and measurement positions are discussed. Simulations show that under the same measurement conditions, the plane wave function method is superior in terms of reconstruction accuracy and data processing efficiency, that is, the entire sound field imaging can be achieved by only one time calculation instead of translations of local sets of coefficients with respect to every measurement position into a global one. An experiment was conducted inside an aircraft cabin mock-up for validation. Additionally, this method provides an alternative possibility to recover the coefficients of high order spherical wave functions in a global coordinate system without coordinate translations with respect to local origins.", "gemini": "Một mảng micrô hình cầu đã chứng minh hiệu quả trong việc tái tạo một trường âm khép kín bằng cách chồng các hàm sóng hình cầu trong miền Fourier. Nó cho phép tái tạo thành công xung quanh mảng, nhưng độ chính xác sẽ giảm dần theo khoảng cách. Để mở rộng quá trình tái tạo hiệu quả ra toàn bộ khoang, một cơ sở sóng phẳng trong miền không gian được sử dụng nhờ đặc tính lan truyền không suy giảm của nó và so sánh với phương pháp hàm sóng hình cầu thông thường trong trường âm tần số thấp bên trong khoang hình trụ. Độ nhạy với nhiễu đo, ảnh hưởng của số lượng sóng phẳng và vị trí đo được thảo luận. Các phép mô phỏng cho thấy rằng trong cùng điều kiện đo, phương pháp hàm sóng phẳng vượt trội hơn về độ chính xác tái tạo và hiệu quả xử lý dữ liệu, tức là có thể đạt được toàn bộ hình ảnh trường âm chỉ bằng một lần tính toán thay vì dịch các tập hợp hệ số cục bộ liên quan đến mọi vị trí đo thành một hệ số toàn cục. Một thí nghiệm đã được tiến hành bên trong mô hình buồng lái máy bay để xác nhận. Ngoài ra, phương pháp này cung cấp một khả năng thay thế để khôi phục các hệ số của các hàm sóng hình cầu bậc cao trong hệ tọa độ toàn cục mà không cần dịch tọa độ liên quan đến các gốc cục bộ."} +{"idx": 1920110, "en": "It is known that the steady acoustic radiation force on a scatterer due to incident time-harmonic waves can be calculated by evaluating certain integrals of velocity potentials over a sphere surrounding the scatterer. The goal is to evaluate these integrals using far-field approximations and appropriate limits. Previous derivations are corrected, clarified, and generalized. Similar corrections are made to textbook derivations of optical theorems.", "gemini": "Người ta biết rằng lực bức xạ âm ổn định trên vật phân tán do sóng điều hòa thời gian tới có thể được tính bằng cách đánh giá các tích phân nhất định của thế vận tốc trên một hình cầu bao quanh vật phân tán. Mục tiêu là đánh giá các tích phân này bằng cách sử dụng các phép xấp xỉ trường xa và các giới hạn thích hợp. Các phép suy biến trước đây được hiệu chỉnh, làm rõ và tổng quát hóa. Các phép hiệu chỉnh tương tự được thực hiện đối với các phép suy biến trong sách giáo khoa về các định lý quang học."} +{"idx": 1920111, "en": "This study compared psychoacoustic reverberance parameters to each other, as well as to reverberation time (RT) and early decay time (EDT) under various acoustic conditions. The psychoacoustic parameters were loudness-based RT (TN), loudness-based EDT [EDTN; Lee, Cabrera, and Martens, J. Acoust. Soc. Am. 131, 1194-1205 (2012a)], and parameter for reverberance [PREV; van Dorp Schuitman, de Vries, and Lindau., J. Acoust. Soc. Am. 133, 1572-1585 (2013)]. For the comparisons, a wide range of sound pressure levels (SPLs) from 20 dB to 100 dB and RTs from 0.5 s to 5.0 s were evaluated, and two sets of subjective data from the previous studies were used for the cross-validation and comparison. Results of the comparisons show that the psychoacoustic reverberance parameters provided better matches to reverberance than RT and EDT; however, the performance of these psychoacoustic reverberance parameters varied with the SPL range, the type of audio sample, and the reverberation conditions. This study reveals that PREV is the most relevant for estimating a relative change in reverberance between samples when the SPL range is small, while EDTN is useful in estimating the absolute reverberance. This study also suggests the use of PREV and EDTN for speech and music samples, respectively.", "gemini": "Nghiên cứu này so sánh các thông số vang âm tâm lý học với nhau, cũng như với thời gian vang (RT) và thời gian phân rã sớm (EDT) trong các điều kiện âm học khác nhau. Các thông số tâm lý học là RT dựa trên độ lớn (TN), EDT dựa trên độ lớn [EDTN; Lee, Cabrera và Martens, J. Acoust. Soc. Am. 131, 1194-1205 (2012a)] và thông số cho độ vang [PREV; van Dorp Schuitman, de Vries và Lindau., J. Acoust. Soc. Am. 133, 1572-1585 (2013)]. Đối với các phép so sánh, một phạm vi rộng các mức áp suất âm thanh (SPL) từ 20 dB đến 100 dB và RT từ 0,5 giây đến 5,0 giây đã được đánh giá và hai bộ dữ liệu chủ quan từ các nghiên cứu trước đó đã được sử dụng để xác nhận chéo và so sánh. Kết quả của các phép so sánh cho thấy các thông số vang âm tâm lý học cung cấp sự phù hợp tốt hơn với độ vang so với RT và EDT; tuy nhiên, hiệu suất của các thông số vang âm tâm lý học này thay đổi theo phạm vi SPL, loại mẫu âm thanh và điều kiện vang. Nghiên cứu này cho thấy PREV có liên quan nhất để ước tính sự thay đổi tương đối về độ vang giữa các mẫu khi phạm vi SPL nhỏ, trong khi EDTN hữu ích trong việc ước tính độ vang tuyệt đối. Nghiên cứu này cũng đề xuất sử dụng PREV và EDTN cho các mẫu giọng nói và nhạc, tương ứng."} +{"idx": 1920112, "en": "Gedeon streaming is known to considerably deteriorate the thermal efficiency of a traveling-wave thermoacoustic engine with looped configuration. The time-average pressure drop induced by a jet pump can efficiently suppress the Gedeon streaming. In this study, such suppression mechanism of the jet pump is investigated, and the emphasis is put on the effects of the dimensionless rounding, the taper angle, and the cross-sectional area ratio. An experimental apparatus has been set up to measure the time-averaged pressure drop induced by the jet pumps in oscillatory flow. Controlled experiments and characterization reveal the time-averaged pressure drop and working efficiency increase with a rise in dimensionless rounding when it is less than 0.15. For jet pumps with the fixed opening areas, the taper angle in the range from 3° to 9° is capable of producing a larger time-averaged pressure drop with a higher working efficiency, and the change of taper angle has little effect on the performance. However, performance degradation is observed as the taper angle increases beyond 9°. Moreover, when the taper angle ranges from 3° to 9°, the time-averaged pressure drop and working efficiency can be improved by increasing the cross-sectional area ratio.", "gemini": "Đã biết rằng sự truyền trực tuyến Gedeon làm giảm đáng kể hiệu suất nhiệt của động cơ nhiệt âm học sóng chạy với cấu hình vòng lặp. Sự giảm áp suất trung bình theo thời gian do bơm tia tạo ra có thể ức chế hiệu quả luồng Gedeon. Trong nghiên cứu này, cơ chế ức chế như vậy của bơm tia được nghiên cứu và nhấn mạnh vào các hiệu ứng của sự làm tròn không có chiều, góc côn và tỷ lệ diện tích mặt cắt. Một bộ máy thí nghiệm đã được thiết lập để đo sự giảm áp suất trung bình theo thời gian do các bơm tia tạo ra trong dòng chảy dao động. Các thí nghiệm có kiểm soát và đặc tính cho thấy sự giảm áp suất trung bình theo thời gian và hiệu suất làm việc tăng lên khi độ tròn không có chiều tăng lên khi nó nhỏ hơn 0,15. Đối với các bơm tia có diện tích mở cố định, góc côn trong phạm vi từ 3° đến 9° có khả năng tạo ra sự giảm áp suất trung bình theo thời gian lớn hơn với hiệu suất làm việc cao hơn và sự thay đổi góc côn có ít ảnh hưởng đến hiệu suất. Tuy nhiên, hiệu suất giảm khi góc côn tăng vượt quá 9°. Hơn nữa, khi góc côn nằm trong khoảng từ 3° đến 9°, sự giảm áp suất trung bình theo thời gian và hiệu suất làm việc có thể được cải thiện bằng cách tăng tỷ lệ diện tích mặt cắt."} +{"idx": 1920113, "en": "Listeners can judge the overall loudness of time-varying sounds quite easily, i.e., assign a single value that represents the loudness of the entire sound. This holds even if the duration is long and the judgment includes memory effects. Different metrics for calculating overall loudness have been developed. They agree that overall loudness is higher than the mean of loudness over time. Percentiles like the N5, the loudness being exceeded 5% of the time, are adopted by ISO 532-1. In the present study the concept of an energy mean known from level measurements (ISO 1996-1) was applied to the loudness domain. This equivalent continuous loudness level, LLP, was compared to the N5 using a set of real-world sounds that was orthogonal between the two metrics over a wide dynamic range of 30 phon. Cross-modality matching with line length was used in three experiments with a focus on either the overall judgment of loudness, continuous judgment while a sound was played, or both. The LLP showed considerably higher correlations with overall judgments than N5. Comparing continuous instantaneous judgment with calculated instantaneous loudness suggests that the participants might have focused on the sounds' prominent portions.", "gemini": "Người nghe có thể đánh giá độ lớn tổng thể của âm thanh thay đổi theo thời gian khá dễ dàng, tức là gán một giá trị duy nhất đại diện cho độ lớn của toàn bộ âm thanh. Điều này đúng ngay cả khi thời lượng dài và phán đoán bao gồm các hiệu ứng bộ nhớ. Các số liệu khác nhau để tính độ lớn tổng thể đã được phát triển. Họ đồng ý rằng độ lớn tổng thể cao hơn độ lớn trung bình theo thời gian. Các phần trăm như N5, độ lớn vượt quá 5% thời gian, được ISO 532-1 áp dụng. Trong nghiên cứu hiện tại, khái niệm về giá trị trung bình năng lượng được biết đến từ các phép đo mức (ISO 1996-1) đã được áp dụng cho miền độ lớn. Mức độ lớn liên tục tương đương này, LLP, đã được so sánh với N5 bằng cách sử dụng một tập hợp các âm thanh trong thế giới thực vuông góc giữa hai số liệu trên một dải động rộng 30 phon. Phù hợp đa phương thức với chiều dài đường đã được sử dụng trong ba thí nghiệm tập trung vào phán đoán tổng thể về độ lớn, phán đoán liên tục trong khi âm thanh được phát hoặc cả hai. LLP cho thấy mối tương quan cao hơn đáng kể với các phán đoán tổng thể so với N5. So sánh phán đoán tức thời liên tục với độ lớn tức thời được tính toán cho thấy những người tham gia có thể tập trung vào các phần nổi bật của âm thanh."} +{"idx": 1920114, "en": "This study investigated articulation of preboundary lengthening (PBL) in tri-syllabic pseudo words (bábaba, babába, bababá) in American English. Results from 10 speakers showed that PBL was modulated by the degree of prominence, i.e., the less prominent, the more PBL. PBL was attracted to the penultimate stressed syllable but only when the word received no pitch accent whereas the antepenultimate syllable showed no PBL. Kinematically, PBL was accompanied by a larger movement along with an increase in peak velocity, showing a kind of boundary-related articulatory strengthening, although there was some evidence of temporal expansion possibly due to lowered stiffness.", "gemini": "Nghiên cứu này đã điều tra sự phát âm của sự kéo dài trước ranh giới (PBL) trong các từ giả ba âm tiết (bábaba, babába, bababá) trong tiếng Anh Mỹ. Kết quả từ 10 người nói cho thấy PBL được điều chỉnh theo mức độ nổi bật, tức là càng ít nổi bật thì PBL càng nhiều. PBL bị thu hút vào âm tiết nhấn mạnh trước cuối nhưng chỉ khi từ không nhận được trọng âm cao độ trong khi âm tiết trước trước cuối không cho thấy PBL. Về mặt động học, PBL đi kèm với chuyển động lớn hơn cùng với sự gia tăng vận tốc cực đại, cho thấy một loại tăng cường phát âm liên quan đến ranh giới, mặc dù có một số bằng chứng về sự giãn nở thời gian có thể là do độ cứng giảm."} +{"idx": 1920115, "en": "A hearing-aid strategy that combines a beamforming microphone array in the high frequencies with natural binaural signals in the low frequencies was examined. This strategy attempts to balance the benefits of beamforming (improved signal-to-noise ratio) with the benefits of binaural listening (spatial awareness and location-based segregation). The crossover frequency was varied from 200 to 1200 Hz, and performance was compared to full-spectrum binaural and beamformer conditions. Speech intelligibility in the presence of noise or competing speech was measured in listeners with and without hearing loss. Results showed that the optimal crossover frequency depended on the listener and the nature of the interference.", "gemini": "Đã kiểm tra một chiến lược trợ thính kết hợp một mảng micrô tạo chùm tia ở tần số cao với các tín hiệu âm thanh nổi tự nhiên ở tần số thấp. Chiến lược này cố gắng cân bằng những lợi ích của việc tạo chùm tia (tỷ lệ tín hiệu trên nhiễu được cải thiện) với những lợi ích của việc nghe âm thanh nổi (nhận thức không gian và phân tách dựa trên vị trí). Tần số giao thoa thay đổi từ 200 đến 1200 Hz và hiệu suất được so sánh với các điều kiện âm thanh nổi phổ toàn và tạo chùm tia. Độ rõ lời nói khi có tiếng ồn hoặc lời nói cạnh tranh được đo ở những người nghe có và không bị mất thính lực. Kết quả cho thấy tần số giao thoa tối ưu phụ thuộc vào người nghe và bản chất của nhiễu."} +{"idx": 1920116, "en": "Most derivations of acoustic wave equations involve ensuring that causality is satisfied. Here, the consequences of also requiring that the medium should be passive are explored. This is a stricter criterion than causality for a linear system and implies that there are restrictions on the relaxation modulus and its first few derivatives. The viscous and relaxation models of acoustics satisfy passivity and have restrictions on not only a few, but all derivatives of the relaxation modulus. These models are described as a system of springs and dampers with positive parameters and belong to the important class of completely monotone systems. It is shown here that the attenuation as a function of frequency for such media has to increase slower than a linear function. Likewise, the phase velocity has to increase monotonically. This gives criteria on which one may judge whether a proposed wave equation is passive or not, as illustrated by comparing two different versions of the viscous wave equation.", "gemini": "Hầu hết các phép đạo hàm của phương trình sóng âm đều liên quan đến việc đảm bảo rằng tính nhân quả được thỏa mãn. Ở đây, hậu quả của việc cũng yêu cầu môi trường phải thụ động được khám phá. Đây là một tiêu chí nghiêm ngặt hơn tính nhân quả đối với một hệ thống tuyến tính và ngụ ý rằng có những hạn chế đối với mô đun độ nhớt và một số đạo hàm đầu tiên của nó. Các mô hình độ nhớt và độ nhớt của âm học đáp ứng tính thụ động và có những hạn chế không chỉ đối với một số mà đối với tất cả các đạo hàm của mô đun độ nhớt. Các mô hình này được mô tả như một hệ thống lò xo và bộ giảm chấn với các thông số dương và thuộc về lớp hệ thống đơn điệu hoàn chỉnh quan trọng. Ở đây cho thấy rằng sự suy giảm như một hàm của tần số đối với các phương tiện như vậy phải tăng chậm hơn một hàm tuyến tính. Tương tự như vậy, vận tốc pha phải tăng đơn điệu. Điều này đưa ra các tiêu chí mà người ta có thể đánh giá xem một phương trình sóng được đề xuất có thụ động hay không, như được minh họa bằng cách so sánh hai phiên bản khác nhau của phương trình sóng độ nhớt."} +{"idx": 1920117, "en": "The field of civil engineering is in need of new methods of non-destructive testing, especially in order to prevent and monitor the serious deterioration of concrete structures. In this work, experimental results are reported on fault detection and characterization in a meter-scale concrete structure using an ultrasonic nonlinear coda wave interferometry (NCWI) method. This method entails the nonlinear mixing of strong pump waves with multiple scattered probe (coda) waves, along with analysis of the net effect using coda wave interferometry. A controlled damage protocol is implemented on a post-tensioned, meter-scale concrete structure in order to generate cracking within a specific area being monitored by NCWI. The nonlinear acoustic response due to the high amplitude of acoustic modulation yields information on the elastic nonlinearities of concrete, as evaluated by two specific nonlinear observables. The increase in nonlinearity level corresponds to the creation of a crack with a network of microcracks localized at its base. In addition, once the crack closes as a result of post-tensioning, the residual nonlinearities confirm the presence of the closed crack. Last, the benefits and applicability of this NCWI method to the characterization and monitoring of large structures are discussed.", "gemini": "Lĩnh vực xây dựng dân dụng đang cần những phương pháp kiểm tra không phá hủy mới, đặc biệt là để ngăn ngừa và theo dõi sự xuống cấp nghiêm trọng của các cấu trúc bê tông. Trong công trình này, các kết quả thực nghiệm được báo cáo về phát hiện và mô tả đặc điểm lỗi trong một cấu trúc bê tông quy mô mét bằng phương pháp giao thoa sóng coda phi tuyến siêu âm (NCWI). Phương pháp này đòi hỏi sự trộn phi tuyến của sóng bơm mạnh với nhiều sóng thăm dò phân tán (coda), cùng với việc phân tích hiệu ứng ròng bằng giao thoa sóng coda. Một giao thức hư hỏng được kiểm soát được thực hiện trên một cấu trúc bê tông quy mô mét sau khi căng để tạo vết nứt trong một khu vực cụ thể đang được NCWI theo dõi. Phản ứng âm thanh phi tuyến do biên độ cao của điều chế âm thanh mang lại thông tin về các phi tuyến tính đàn hồi của bê tông, được đánh giá bằng hai quan sát phi tuyến cụ thể. Sự gia tăng mức độ phi tuyến tính tương ứng với sự tạo ra một vết nứt với một mạng lưới các vết nứt nhỏ tập trung ở đáy của nó. Ngoài ra, một khi vết nứt đóng lại do hậu căng, thì các phi tuyến tính còn lại xác nhận sự hiện diện của vết nứt đã đóng. Cuối cùng, các lợi ích và khả năng áp dụng của phương pháp NCWI này vào việc mô tả đặc điểm và theo dõi các cấu trúc lớn được thảo luận."} +{"idx": 1920118, "en": "A chirp signal is a signal with linearly varying instantaneous frequency over the signal bandwidth, also known as a linear frequency modulated (LFM) signal. It is widely used in communication, radar, active sonar, and other applications due to its Doppler tolerance property in signal detection using the matched filter (MF) processing. Modern sonar uses high-gain, wideband signals to improve the signal to reverberation ratio. High gain implies a high product of the signal bandwidth and duration. However, wideband and/or long duration LFM signals are no longer Doppler tolerant. The shortcoming of the standard MF processing is loss of performance, and bias in range estimation. This paper uses the wideband ambiguity function and the fractional Fourier transform method to estimate the target velocity and restore the performance. Target velocity or Doppler provides a clue for differentiating the target from the background reverberation and clutter. The methods are applied to simulated and experimental data.", "gemini": "Tín hiệu chirp là tín hiệu có tần số tức thời thay đổi tuyến tính trên băng thông tín hiệu, còn được gọi là tín hiệu điều chế tần số tuyến tính (LFM). Nó được sử dụng rộng rãi trong truyền thông, radar, sonar chủ động và các ứng dụng khác do tính chất chịu Doppler của nó trong phát hiện tín hiệu sử dụng xử lý bộ lọc phù hợp (MF). Sonar hiện đại sử dụng các tín hiệu băng thông rộng, độ lợi cao để cải thiện tỷ lệ tín hiệu trên nhiễu. Độ lợi cao ngụ ý tích của băng thông tín hiệu và thời lượng cao. Tuy nhiên, các tín hiệu LFM băng thông rộng và/hoặc thời lượng dài không còn chịu được Doppler. Hạn chế của xử lý MF tiêu chuẩn là mất hiệu suất và độ lệch trong ước tính phạm vi. Bài báo này sử dụng hàm mơ hồ băng thông rộng và phương pháp biến đổi Fourier phân số để ước tính vận tốc mục tiêu và khôi phục hiệu suất. Vận tốc mục tiêu hoặc Doppler cung cấp một manh mối để phân biệt mục tiêu với nhiễu nền và nhiễu. Các phương pháp được áp dụng cho dữ liệu mô phỏng và thực nghiệm."} +{"idx": 1920119, "en": "Acoustic properties of odontocete head tissues, including sound velocity, density, and acoustic impedance, are important parameters to understand dynamics of its echolocation. In this paper, acoustic properties of head tissues from a freshly dead short-finned pilot whale (Globicephala macrorhynchus) were reconstructed using computed tomography (CT) and ultrasound. The animal's forehead soft tissues were cut into 188 ordered samples. Sound velocity, density, and acoustic impedance of each sample were either directly measured or calculated by formula, and Hounsfield Unit values (HUs) were obtained from CT scanning. According to relationships between HUs and sound velocity, HUs and density, as well as HUs and acoustic impedance, distributions of acoustic properties in the head were reconstructed. The inner core in the melon with low-sound velocity and low-density is an evidence for its potential function of sound focusing. The increase in acoustic impedance of forehead tissues from inner core to outer layer may be important for the acoustic impedance matching between the outer layer tissue and seawater. In addition, temperature dependence of sound velocity in soft tissues was also examined. The results provide a guide to the simulation of the sound emission of the short-finned pilot whale.", "gemini": "Các đặc tính âm học của mô đầu cá heo răng, bao gồm vận tốc âm thanh, mật độ và trở kháng âm thanh, là những thông số quan trọng để hiểu động lực định vị bằng tiếng vang của nó. Trong bài báo này, các đặc tính âm học của mô đầu từ một con cá voi phi công vây ngắn mới chết (Globicephala macrorhynchus) đã được tái tạo bằng chụp cắt lớp vi tính (CT) và siêu âm. Các mô mềm trên trán của động vật được cắt thành 188 mẫu có thứ tự. Vận tốc âm thanh, mật độ và trở kháng âm thanh của mỗi mẫu được đo trực tiếp hoặc tính toán theo công thức và giá trị đơn vị Hounsfield (HU) thu được từ quét CT. Theo các mối quan hệ giữa HU và vận tốc âm thanh, HU và mật độ, cũng như HU và trở kháng âm thanh, phân bố các đặc tính âm học trong đầu được tái tạo lại. Lõi bên trong ở quả dưa với vận tốc âm thanh thấp và mật độ thấp là bằng chứng cho chức năng tiềm ẩn của nó là hội tụ âm thanh. Sự gia tăng trở kháng âm thanh của các mô trán từ lõi bên trong đến lớp ngoài có thể quan trọng đối với sự phù hợp trở kháng âm thanh giữa mô lớp ngoài và nước biển. Ngoài ra, sự phụ thuộc nhiệt độ của vận tốc âm thanh trong các mô mềm cũng được kiểm tra. Các kết quả thu được cung cấp hướng dẫn cho việc mô phỏng phát xạ âm thanh của cá voi phi công vây ngắn."} +{"idx": 1920120, "en": "The waveguide invariant β is typically discussed in terms of either a range-independent environment or a range-dependent environment under the adiabatic approximation, with few studies considering the effects of mode coupling. In this work, how internal solitary waves (ISWs) affect the waveguide invariant is investigated, and it is shown that mode-coupling effects introduce many additional components in the acoustic interference intensity. It is found that the striation slope and value of β for these additional components are determined not only by the acoustic modal dispersion, but are also dependent on the position where the mode coupling occurs. This can lead to a very complicated acoustic interference pattern and result in multiple peaks in the distribution of β. The sensitivity of β to the parameters of ISWs, such as amplitude, horizontal scale, and position, is analyzed. It is found that although all parameters can affect the energy of the peaks, only the position of the internal wave has an obvious impact on the peak values. This indicates that the peak values of β can be utilized for monitoring the position of the internal wave.", "gemini": "Bất biến β của ống dẫn sóng thường được thảo luận theo khái niệm về môi trường không phụ thuộc vào phạm vi hoặc môi trường phụ thuộc vào phạm vi theo phép xấp xỉ đoạn nhiệt, với một số nghiên cứu xem xét tác động của chế độ ghép nối. Trong công trình này, cách sóng đơn độc bên trong (ISW) ảnh hưởng đến bất biến của ống dẫn sóng được nghiên cứu và người ta chỉ ra rằng các hiệu ứng ghép nối chế độ đưa vào nhiều thành phần bổ sung trong cường độ giao thoa âm thanh. Người ta thấy rằng độ dốc và giá trị của β đối với các thành phần bổ sung này không chỉ được xác định bởi sự phân tán mô thức âm thanh mà còn phụ thuộc vào vị trí xảy ra ghép nối chế độ. Điều này có thể dẫn đến một mô hình giao thoa âm thanh rất phức tạp và tạo ra nhiều đỉnh trong phân bố β. Độ nhạy của β đối với các thông số của ISW, chẳng hạn như biên độ, tỷ lệ ngang và vị trí, được phân tích. Người ta thấy rằng mặc dù tất cả các thông số đều có thể ảnh hưởng đến năng lượng của các đỉnh, nhưng chỉ có vị trí của sóng bên trong có tác động rõ ràng đến các giá trị đỉnh. Điều này chỉ ra rằng các giá trị đỉnh của β có thể được sử dụng để theo dõi vị trí của sóng bên trong."} +{"idx": 1920121, "en": "The application of the root multiple signal classification algorithm for raypath separation was motivated by the dramatic reduction in processing time of the multiple-signal classification algorithm. However, the algorithm provides classification only in the direction of the arrival domain and fails to separate raypaths arriving at the array with similar directions of arrival. Moreover, for many applications in shallow water (such as ocean acoustic tomography and active sonar), the emitted signal is known and can be used as a priori information to improve the resolution. Thus, in this study, a two-dimensional active wideband classification algorithm is developed using the examination of the roots of the spectrum polynomial in the angle versus time domain. A two-step strategy is developed to enable extension to the two-dimensional case. The results of simulations confirm that the proposed algorithm achieves almost identical resolution as the existing two-dimensional algorithms while offering a significant reduction in computation time.", "gemini": "Việc áp dụng thuật toán phân loại tín hiệu bội số gốc để phân tách đường đi tia được thúc đẩy bởi sự giảm đáng kể thời gian xử lý của thuật toán phân loại tín hiệu bội số. Tuy nhiên, thuật toán chỉ cung cấp phân loại theo hướng của miền đến và không phân tách được các đường đi tia đến mảng với các hướng đến tương tự. Hơn nữa, đối với nhiều ứng dụng trong vùng nước nông (như chụp cắt lớp âm thanh đại dương và sonar chủ động), tín hiệu phát ra được biết đến và có thể được sử dụng làm thông tin trước để cải thiện độ phân giải. Do đó, trong nghiên cứu này, một thuật toán phân loại băng thông rộng chủ động hai chiều được phát triển bằng cách kiểm tra các gốc của đa thức phổ trong miền góc so với thời gian. Một chiến lược hai bước được phát triển để cho phép mở rộng sang trường hợp hai chiều. Kết quả mô phỏng xác nhận rằng thuật toán được đề xuất đạt độ phân giải gần như giống hệt với các thuật toán hai chiều hiện có trong khi giảm đáng kể thời gian tính toán."} +{"idx": 1920122, "en": "Arterial stenosis is a vascular pathology which leads to serious cardiovascular diseases. Blood flow through a constriction generates sound and vibration due to fluctuating turbulent pressures. Generated vibro-acoustic waves propagate through surrounding soft tissues and reach the skin surface and may provide valuable insight for noninvasive diagnostic purposes. Motivated by the aforementioned phenomena, vibration of constricted arteries is investigated employing computational models. The flow-induced pressure field in an artery is modeled as broadband harmonic pressure loading based on previous studies in the literature and applied on the inner artery wall. Harmonic analysis is performed for determining radial velocity responses on the outer surface of the models. Results indicate that stenosis severities higher than 70% lead to significant increase in response amplitudes, especially at high frequencies between 250 and 600 Hz. The findings agree well with experimental and theoretical results in the literature considering bending mode frequencies, amplitude scales, and mainly excited frequency ranges. It is seen that artery vibration is sensitive to the phase behavior of pressure loading but its effect becomes less significant with the presence of surrounding tissue. As the surrounding tissue thickness increases, radial velocity response amplitudes decrease but the effect of changes in tissue elastic modulus is more pronounced.", "gemini": "Hẹp động mạch là một bệnh lý mạch máu dẫn đến các bệnh tim mạch nghiêm trọng. Dòng máu chảy qua chỗ thắt tạo ra âm thanh và rung động do áp suất nhiễu động dao động. Sóng rung động âm thanh được tạo ra lan truyền qua các mô mềm xung quanh và đến bề mặt da và có thể cung cấp thông tin chi tiết có giá trị cho mục đích chẩn đoán không xâm lấn. Được thúc đẩy bởi các hiện tượng đã đề cập ở trên, độ rung của động mạch bị thắt được nghiên cứu bằng cách sử dụng các mô hình tính toán. Trường áp suất do dòng chảy tạo ra trong động mạch được mô hình hóa như tải áp suất hài băng thông rộng dựa trên các nghiên cứu trước đây trong tài liệu và được áp dụng trên thành động mạch bên trong. Phân tích hài được thực hiện để xác định các phản ứng vận tốc hướng kính trên bề mặt ngoài của các mô hình. Kết quả chỉ ra rằng mức độ hẹp động mạch cao hơn 70% dẫn đến tăng đáng kể biên độ phản ứng, đặc biệt ở tần số cao từ 250 đến 600 Hz. Các phát hiện này phù hợp với các kết quả thực nghiệm và lý thuyết trong tài liệu khi xem xét tần số chế độ uốn cong, thang biên độ và chủ yếu là các dải tần số kích thích. Có thể thấy rằng độ rung của động mạch nhạy cảm với hành vi pha của tải áp suất nhưng tác động của nó trở nên ít đáng kể hơn khi có mô xung quanh. Khi độ dày mô xung quanh tăng lên, biên độ phản ứng vận tốc hướng kính giảm nhưng tác động của sự thay đổi mô đun đàn hồi mô rõ rệt hơn."} +{"idx": 1920123, "en": "Up to medium intensities and in the 80-100-Hz region, the auditory steady-state response (ASSR) to a multi-tone carrier is commonly considered to be a linear sum of the dipoles from each tone specific ASSR generator. Here, this hypothesis was investigated when a unique modulation frequency is used for all carrier components. Listeners were presented with a co-modulated dual-frequency carrier (1 and 4 kHz), from which the modulator starting phase Φi of the 1-kHz component was systematically varied. The results support the hypothesis of a linear superposition of the dipoles originating from different frequency specific ASSR generators.", "gemini": "Với cường độ trung bình và trong vùng 80-100-Hz, phản ứng trạng thái ổn định thính giác (ASSR) đối với sóng mang đa âm thường được coi là tổng tuyến tính của các lưỡng cực từ mỗi máy phát ASSR đặc hiệu từng âm. Ở đây, giả thuyết này đã được nghiên cứu khi sử dụng tần số điều chế duy nhất cho tất cả các thành phần sóng mang. Người nghe được trình bày với sóng mang tần số kép được điều chế đồng thời (1 và 4 kHz), từ đó pha khởi động của bộ điều chế Φi của thành phần 1 kHz được thay đổi một cách có hệ thống. Kết quả ủng hộ giả thuyết về sự chồng chất tuyến tính của các lưỡng cực có nguồn gốc từ các máy phát ASSR đặc hiệu tần số khác nhau."} +{"idx": 1920124, "en": "One of the most widely studied observations in linguistic phonetics is that, all else being equal, vowels are longer before voiced than before voiceless obstruents. The causes of this phonetic generalization are, however, poorly understood and several competing explanations have been proposed. No studies have so far measured vowel duration before stops with yet another laryngeal feature: ejectives. This study fills this gap and presents results from an experiment that measures vowel duration before stops with all three laryngeal features in Georgian and models effects of both closure and voice onset time (VOT) on preceding vowel duration at the same time. The results show that vowels have significantly different durations before all three series of stops, voiced, ejective, and voiceless aspirated, even when closure and VOT durations are controlled for. The results also suggest that closure and VOT durations are inversely correlated with preceding vowel duration. These results combined bear several implications for the discussion of causes of vowel duration differences: the data support the hypotheses that claim that laryngeal gestures, temporal compensation, and closure velocity affect vowel duration. Some explanations, especially perceptual and airflow expenditure explanations, are considerably weakened by the results.", "gemini": "Một trong những quan sát được nghiên cứu rộng rãi nhất trong ngữ âm học ngôn ngữ là, xét về mọi mặt, các nguyên âm dài hơn trước các âm tắc hữu thanh so với trước các âm tắc vô thanh. Tuy nhiên, nguyên nhân của sự khái quát ngữ âm này vẫn chưa được hiểu rõ và một số giải thích cạnh tranh đã được đưa ra. Cho đến nay, không có nghiên cứu nào đo được độ dài nguyên âm trước các âm tắc với một đặc điểm thanh quản khác: âm bật hơi. Nghiên cứu này lấp đầy khoảng trống này và trình bày kết quả từ một thí nghiệm đo độ dài nguyên âm trước các âm tắc với cả ba đặc điểm thanh quản trong tiếng Gruzia và mô hình hóa tác động của cả thời gian bắt đầu đóng và phát âm (VOT) lên độ dài nguyên âm trước đó cùng một lúc. Kết quả cho thấy các nguyên âm có độ dài khác nhau đáng kể trước cả ba loạt âm tắc, hữu thanh, bật hơi và vô thanh được hít vào, ngay cả khi thời gian đóng và VOT được kiểm soát. Kết quả cũng cho thấy thời gian đóng và VOT có mối tương quan nghịch với độ dài nguyên âm trước đó. Những kết quả này kết hợp có một số ý nghĩa đối với cuộc thảo luận về nguyên nhân gây ra sự khác biệt về độ dài nguyên âm: dữ liệu ủng hộ các giả thuyết cho rằng cử chỉ thanh quản, sự bù trừ về thời gian và vận tốc đóng ảnh hưởng đến độ dài nguyên âm. Một số giải thích, đặc biệt là các giải thích về nhận thức và chi tiêu luồng không khí, đã bị các kết quả làm suy yếu đáng kể."} +{"idx": 1920125, "en": "This paper deals with the loss of coherence in underwater direction-of-arrival estimation. The coherence loss, which typically arises from dynamical ocean fluctuations and unknown environmental parameters, may take the form of a multiplicative colored random noise applied to the measured acoustic signal. This specific multiplicative noise needs to be addressed with methodological developments. This paper proposes a weighting process that locally mitigates the effects of the coherence loss. More specially, a set of coherent sub-antennas is designed from the so-called Mutual Coherence Function (MCF). The assessed source position results from the combination of each sub-antenna by using a mixed norm. Two experiments are considered in the paper: either a random noise is sampled to simulate the effect of random ocean fluctuations, or a synthetic acoustic waveguide is used in which the coherence loss is due to some multipath interferences. It is shown that the weighting process allows for a decrease in the estimation error in comparison to a Conventional Beamformer (CB).", "gemini": "Bài báo này đề cập đến sự mất tính kết hợp trong ước tính hướng đến của dưới nước. Sự mất tính kết hợp, thường phát sinh từ các biến động của đại dương động và các thông số môi trường chưa biết, có thể có dạng nhiễu ngẫu nhiên màu nhân với tín hiệu âm thanh được đo. Cần phải giải quyết loại nhiễu nhân cụ thể này bằng các phương pháp phát triển. Bài báo này đề xuất một quy trình gia quyền làm giảm cục bộ các tác động của sự mất tính kết hợp. Đặc biệt hơn, một tập hợp các ăng ten phụ kết hợp được thiết kế từ cái gọi là Hàm kết hợp hỗ tương (MCF). Vị trí nguồn được đánh giá là kết quả từ sự kết hợp của từng ăng ten phụ bằng cách sử dụng chuẩn hỗn hợp. Bài báo xem xét hai thí nghiệm: hoặc một nhiễu ngẫu nhiên được lấy mẫu để mô phỏng tác động của các biến động ngẫu nhiên của đại dương, hoặc một ống dẫn sóng âm thanh tổng hợp được sử dụng trong đó sự mất tính kết hợp là do một số nhiễu đa đường. Kết quả cho thấy quy trình gia quyền cho phép giảm lỗi ước tính so với Bộ tạo chùm tia thông thường (CB)."} +{"idx": 1920126, "en": "The scattering of sound by a membrane-covered cavity in a duct with shear flow is calculated with a linear model based on the multimodal method. The model is verified by comparison against the previous experiments focused on sound suppression of a stable system with high-tension membranes and a low-speed flow. It is shown in this paper that such a situation is drastically changed when the flow velocity is larger than the in vacuo flexural wave speed of the membrane. One of the neutral hydrodynamic modes can be destabilized under certain conditions, and this flexural instability can lead to sound amplification. For a given flow profile, the axial growth rate of the instability increases with the mean flow velocity but saturates at high velocities. For a given mean flow velocity, there is an optimum boundary layer thickness for the instability. Increasing the structural damping tends to stabilize the instability and thus inhibit the sound amplification.", "gemini": "Sự tán xạ âm thanh bởi khoang phủ màng trong ống dẫn có dòng chảy cắt được tính toán bằng mô hình tuyến tính dựa trên phương pháp đa phương thức. Mô hình được xác minh bằng cách so sánh với các thí nghiệm trước đó tập trung vào việc triệt tiêu âm thanh của hệ thống ổn định có màng căng cao và dòng chảy tốc độ thấp. Bài báo này cho thấy tình huống như vậy thay đổi rất nhiều khi vận tốc dòng chảy lớn hơn vận tốc sóng uốn của màng trong chân không. Một trong các chế độ thủy động lực học trung hòa có thể mất ổn định trong một số điều kiện nhất định và sự mất ổn định uốn này có thể dẫn đến khuếch đại âm thanh. Đối với một cấu hình dòng chảy nhất định, tốc độ tăng trưởng trục của sự mất ổn định tăng theo vận tốc dòng chảy trung bình nhưng bão hòa ở vận tốc cao. Đối với một vận tốc dòng chảy trung bình nhất định, có một độ dày lớp ranh giới tối ưu cho sự mất ổn định. Việc tăng độ giảm chấn cấu trúc có xu hướng ổn định sự mất ổn định và do đó ức chế sự khuếch đại âm thanh."} +{"idx": 1920127, "en": "Many cases of disturbed voice signals can be attributed to incomplete glottal closure, vocal fold oscillation asymmetries, and aperiodicity. Often these phenomena occur simultaneously and interact with each other, making a systematic, isolated investigation challenging. Therefore, ex vivo porcine experiments were performed which enable direct control of glottal configurations. Different pre-phonatory glottal gap sizes, adduction levels, and flow rates were adjusted. The resulting glottal closure types were identified in a post-processing step. Finally, the acoustic quality, aerodynamic parameters, and the characteristics of vocal fold oscillation were analyzed in reference to the glottal closure types. Results show that complete glottal closure stabilizes the phonation process indicated through a reduced left-right phase asymmetry, increased amplitude and time periodicity, and an increase in the acoustic quality. Although asymmetry and periodicity parameter variation covers only a small range of absolute values, these small variations have a remarkable influence on the acoustic quality. Due to the fact that these parameters cannot be influenced directly, the authors suggest that the (surgical) reduction of the glottal gap seems to be a promising method to stabilize the phonatory process, which has to be confirmed in future studies.", "gemini": "Nhiều trường hợp tín hiệu giọng nói bị nhiễu có thể là do đóng thanh môn không hoàn toàn, dao động không đối xứng của nếp gấp thanh âm và không tuần hoàn. Thường thì những hiện tượng này xảy ra đồng thời và tương tác với nhau, khiến cho việc điều tra riêng biệt, có hệ thống trở nên khó khăn. Do đó, các thí nghiệm trên lợn đã được thực hiện cho phép kiểm soát trực tiếp cấu hình thanh môn. Các kích thước khe hở thanh môn trước khi phát âm, mức độ khép và tốc độ dòng chảy khác nhau đã được điều chỉnh. Các loại đóng thanh môn thu được đã được xác định trong bước xử lý hậu kỳ. Cuối cùng, chất lượng âm thanh, các thông số khí động học và các đặc điểm dao động của nếp gấp thanh âm đã được phân tích để tham chiếu đến các loại đóng thanh môn. Kết quả cho thấy việc đóng thanh môn hoàn toàn làm ổn định quá trình phát âm được chỉ ra thông qua sự bất đối xứng pha trái-phải giảm, biên độ và chu kỳ thời gian tăng và chất lượng âm thanh tăng. Mặc dù sự bất đối xứng và biến thiên thông số chu kỳ chỉ bao phủ một phạm vi nhỏ các giá trị tuyệt đối, nhưng những biến thiên nhỏ này có ảnh hưởng đáng kể đến chất lượng âm thanh. Do thực tế là không thể ảnh hưởng trực tiếp đến các thông số này, các tác giả cho rằng việc giảm (phẫu thuật) khe hở thanh môn dường như là một phương pháp đầy hứa hẹn để ổn định quá trình phát âm, điều này phải được xác nhận trong các nghiên cứu tiếp theo."} +{"idx": 1920128, "en": "A progressive spherical or spheroidal wavefront approximation has previously been found to be a necessary step for a more accurate application of Webster's wave equation to rapidly flaring horns. This leads to a necessary transformation of the horn area function, from the usual flat cross-sectional area in terms of the axial coordinate, into a curved cap-like wavefront area as a function of either the axial coordinate, the arc-length coordinate along the horn profile, the leading curved wavefront coordinate, or still other possible longitudinal coordinates. In this article, horn functions, and related frequency potential functions are calculated from the measured horn profiles of a trombone and a trumpet for several of the above parameterizations. From them, cutoff frequencies and effective lengths are determined. A comparison is drawn between theoretical results using different parameterizations, results calculated via transfer-matrix models, and experimental measurements of the acoustical input impedance and reflection function of both instruments. Results indicate that one-dimensional models accurately predict the effective lengths, and consequently the fundamental resonance frequency of the instruments within ±25 cents, but fail noticeably in predicting cutoff frequencies, leading to what is probably an inaccurate representation of perceived timbre.", "gemini": "Trước đây, người ta đã tìm thấy một phép xấp xỉ mặt sóng hình cầu hoặc hình cầu tiến triển là một bước cần thiết để áp dụng chính xác hơn phương trình sóng của Webster vào các còi loe nhanh. Điều này dẫn đến sự biến đổi cần thiết của hàm diện tích còi, từ diện tích mặt cắt phẳng thông thường theo tọa độ trục, thành diện tích mặt sóng cong như một nắp như một hàm của tọa độ trục, tọa độ độ dài vòng cung dọc theo hồ sơ còi, tọa độ mặt sóng cong dẫn đầu hoặc các tọa độ dọc khác có thể có. Trong bài viết này, các hàm còi và các hàm thế tần số liên quan được tính toán từ các hồ sơ còi được đo của kèn trombone và kèn trumpet cho một số tham số hóa ở trên. Từ đó, tần số cắt và độ dài hiệu dụng được xác định. Một sự so sánh được rút ra giữa các kết quả lý thuyết sử dụng các tham số hóa khác nhau, các kết quả được tính toán thông qua các mô hình ma trận truyền và các phép đo thực nghiệm về trở kháng đầu vào âm thanh và hàm phản xạ của cả hai nhạc cụ. Kết quả chỉ ra rằng các mô hình một chiều dự đoán chính xác độ dài hiệu dụng và do đó tần số cộng hưởng cơ bản của các nhạc cụ trong phạm vi ±25 cent, nhưng lại không dự đoán được tần số cắt, dẫn đến khả năng biểu diễn không chính xác về âm sắc được cảm nhận."} +{"idx": 1920129, "en": "Meaningful sounds represent the majority of sounds that humans hear and process in everyday life. Yet studies of human sound localization mainly use artificial stimuli such as clicks, pure tones, and noise bursts. The present study investigated the influence of behavioral relevance, sound category, and acoustic properties on the localization of complex, meaningful sounds in the horizontal plane. Participants localized vocalizations and traffic sounds with two levels of behavioral relevance (low and high) within each category, as well as amplitude-modulated tones. Results showed a small but significant effect of behavioral relevance: localization acuity was higher for complex sounds with a high level of behavioral relevance at several target locations. The data also showed category-specific effects: localization biases were lower, and localization precision higher, for vocalizations than for traffic sounds in central space. Several acoustic parameters influenced sound localization performance as well. Correcting localization responses for front-back reversals reduced the overall variability across sounds, but behavioral relevance and sound category still had a modulatory effect on sound localization performance in central auditory space. The results thus demonstrate that spatial hearing performance for complex sounds is influenced not only by acoustic characteristics, but also by sound category and behavioral relevance.", "gemini": "Những âm thanh có nghĩa chiếm phần lớn các âm thanh mà con người nghe và xử lý trong cuộc sống hàng ngày. Tuy nhiên, các nghiên cứu về định vị âm thanh của con người chủ yếu sử dụng các kích thích nhân tạo như tiếng click, âm thanh thuần túy và tiếng nổ. Nghiên cứu hiện tại đã điều tra ảnh hưởng của sự liên quan về hành vi, loại âm thanh và các đặc tính âm học đối với việc định vị các âm thanh phức tạp, có nghĩa trong mặt phẳng ngang. Những người tham gia đã xác định vị trí của các âm thanh phát ra từ giọng nói và giao thông với hai mức độ liên quan đến hành vi (thấp và cao) trong mỗi loại, cũng như các âm có biên độ điều chế. Kết quả cho thấy một hiệu ứng nhỏ nhưng đáng kể về sự liên quan đến hành vi: độ nhạy định vị cao hơn đối với các âm thanh phức tạp có mức độ liên quan đến hành vi cao ở một số vị trí mục tiêu. Dữ liệu cũng cho thấy các hiệu ứng cụ thể theo loại: độ lệch định vị thấp hơn và độ chính xác định vị cao hơn đối với âm thanh phát ra từ giọng nói so với âm thanh giao thông ở không gian trung tâm. Một số thông số âm học cũng ảnh hưởng đến hiệu suất định vị âm thanh. Việc hiệu chỉnh các phản ứng định vị cho các đảo ngược trước-sau đã giảm được độ biến thiên tổng thể giữa các âm thanh, nhưng sự liên quan đến hành vi và loại âm thanh vẫn có tác dụng điều chỉnh hiệu suất định vị âm thanh trong không gian thính giác trung tâm. Do đó, các kết quả chứng minh rằng hiệu suất nghe không gian đối với các âm thanh phức tạp không chỉ bị ảnh hưởng bởi các đặc điểm âm học mà còn bị ảnh hưởng bởi loại âm thanh và sự liên quan đến hành vi."} +{"idx": 1920130, "en": "Acoustic signal production is affected by allometric relationships, by which the larger the animal, the lower its call frequency. In this paper, three evolutionary acoustic hypotheses were tested: the Signal-to-Noise Ratio Hypothesis (SNRH), in which evolution maximizes call ranges by increasing the signal-to-noise ratio; the Stimulus Threshold Hypothesis (STH), in which evolution maximizes the range of a specific signal threshold; and the Body Size Hypothesis (BSH), in which the emission of long wavelengths is enabled by body size. Three spectral metrics were measured, Dominant Frequency (FDOM), Minimum Fundamental Frequencies (FFMIN), and Maximum Fundamental Frequencies (FFMAX) of Neotropical Parrots, New World Doves, Woodcreepers, Tinamous, and Thrushes. A Ranged Major Axis (RMA) regression showed that body mass is significantly correlated with all of the spectral parameters in Parrots, Doves, and Woodcreepers, but only with the fundamental frequencies of Tinamous. The FDOM of Parrots corroborated the SNRH. The FFMIN of Woodcreepers and Tinamous corroborated the SNRH and BSH. The FFMAX of Parrots corroborated the STH and BSH. Those acoustic hypotheses could shed light on the evolutionary processes involved in avian communication, although results indicate that these depend on the taxa and spectral parameters considered.", "gemini": "Việc tạo ra tín hiệu âm thanh bị ảnh hưởng bởi các mối quan hệ dị hình, theo đó, động vật càng lớn thì tần số gọi của chúng càng thấp. Trong bài báo này, ba giả thuyết tiến hóa về âm học đã được kiểm tra: Giả thuyết về Tỷ lệ tín hiệu trên nhiễu (SNRH), trong đó quá trình tiến hóa tối đa hóa phạm vi cuộc gọi bằng cách tăng tỷ lệ tín hiệu trên nhiễu; Giả thuyết ngưỡng kích thích (STH), trong đó quá trình tiến hóa tối đa hóa phạm vi ngưỡng tín hiệu cụ thể; và Giả thuyết về kích thước cơ thể (BSH), trong đó việc phát ra các bước sóng dài được kích hoạt bởi kích thước cơ thể. Ba số liệu quang phổ đã được đo, Tần số trội (FDOM), Tần số cơ bản tối thiểu (FFMIN) và Tần số cơ bản tối đa (FFMAX) của Vẹt Tân nhiệt đới, Bồ câu Tân thế giới, Chim trèo cây, Tinamous và Chim họa mi. Phân hồi hồi quy Trục chính có phạm vi (RMA) cho thấy khối lượng cơ thể có mối tương quan đáng kể với tất cả các thông số quang phổ ở Vẹt, Bồ câu và Chim trèo cây, nhưng chỉ tương quan với các tần số cơ bản của Tinamous. FDOM của Vẹt đã xác nhận SNRH. FFMIN của Chim trèo cây và Tinamous đã xác nhận SNRH và BSH. FFMAX của Vẹt đã xác nhận STH và BSH. Những giả thuyết về âm học đó có thể làm sáng tỏ các quá trình tiến hóa liên quan đến giao tiếp của loài chim, mặc dù kết quả chỉ ra rằng chúng phụ thuộc vào các loài và các thông số quang phổ được xem xét."} +{"idx": 1920131, "en": "Sound propagation over the ground with a random spatially-varying surface admittance is investigated. Starting from the Green's theorem, a Dyson equation is derived for the coherent acoustic pressure. Under the Bourret approximation, an explicit expression is deduced and an effective admittance that depends on the correlation function of the admittance fluctuations is exhibited. An asymptotic expression at long range is then obtained. Influence of the randomness on the amplitude of the reflection coefficient and on the wavenumbers of the surface wave component is analyzed. Afterwards, numerical simulations of the linearized Euler equations are carried out and the coherent pressure obtained by an ensemble-averaging over 200 realizations of the admittance is found to be in good agreement with the analytical solution. In the considered examples of grounds, the mean intensity is shown to be similar to the intensity in the non-random case, except near interferences that are smoothened out due to randomness. It is however exemplified that the intensity fluctuations can be large, especially near destructive interferences.", "gemini": "Truyền âm trên mặt đất với độ chấp nhận bề mặt thay đổi ngẫu nhiên theo không gian được nghiên cứu. Bắt đầu từ định lý Green, một phương trình Dyson được suy ra cho áp suất âm thanh mạch lạc. Theo phép xấp xỉ Bourret, một biểu thức rõ ràng được suy ra và một độ chấp nhận hiệu quả phụ thuộc vào hàm tương quan của các biến động độ chấp nhận được thể hiện. Sau đó, một biểu thức tiệm cận ở tầm xa được thu được. Ảnh hưởng của tính ngẫu nhiên đến biên độ của hệ số phản xạ và số sóng của thành phần sóng bề mặt được phân tích. Sau đó, các mô phỏng số của các phương trình Euler tuyến tính được thực hiện và áp suất mạch lạc thu được bằng cách tính trung bình trên tổng thể 200 lần thực hiện độ chấp nhận được thấy là phù hợp với giải pháp phân tích. Trong các ví dụ được xem xét về mặt đất, cường độ trung bình được chứng minh là tương tự như cường độ trong trường hợp không ngẫu nhiên, ngoại trừ gần các nhiễu xạ được làm mịn do tính ngẫu nhiên. Tuy nhiên, cường độ biến động có thể lớn, đặc biệt là gần các nhiễu xạ phá hoại."} +{"idx": 1920132, "en": "Sound transmission and resulting airway wall vibration in a complex multiscale viscoelastic model of the subglottal bronchial tree was calculated using a modified one-dimensional (1D) branching acoustic waveguide approach. This is an extension of previous work to enable use of complex airway trees that are partially derived from subject-specific medical images, without the need for self-similarity in the geometric structure. The approach was validated numerically for simplified airway geometries, as well as experimentally by comparison to previous studies. A comprehensive conducting airway tree with about 60 000 branches was then modified to create fibrotic, bronchoconstrictive, and pulmonary infiltrate conditions. The fibrotic case-systemic increase in soft tissue stiffness-increased the Helmholtz resonance frequency due to the increased acoustic impedance. Bronchoconstriction, with geometric changes in small conducting airways, decreased acoustic energy transmission to the peripheral airways due in part to the increased impedance mismatch between airway orders. Pulmonary infiltrate significantly altered the local acoustic field in the affected lobe. Calculation of acoustic differences between healthy versus pathologic cases can be used to enhance the understanding of vibro-acoustic changes correlated to pathology, and potentially provide improved tools for the diagnosis of pulmonary diseases that uniquely alter the acoustics of the airways.", "gemini": "Truyền âm thanh và rung động thành của đường thở kết quả trong một mô hình nhớt đàn hồi đa thang phức tạp của cây phế quản dưới thanh môn được tính toán bằng cách sử dụng một phương pháp tiếp cận ống dẫn sóng âm phân nhánh một chiều (1D) đã được sửa đổi. Đây là phần mở rộng của công trình trước đây để cho phép sử dụng các cây đường thở phức tạp được lấy một phần từ hình ảnh y tế cụ thể của đối tượng, mà không cần sự tự tương tự trong cấu trúc hình học. Phương pháp tiếp cận đã được xác nhận về mặt số đối với hình học đường thở được đơn giản hóa, cũng như theo phương pháp thực nghiệm bằng cách so sánh với các nghiên cứu trước đây. Sau đó, một cây đường thở dẫn truyền toàn diện với khoảng 60.000 nhánh đã được sửa đổi để tạo ra các tình trạng xơ hóa, co thắt phế quản và thâm nhiễm phổi. Trường hợp xơ hóa-tăng toàn thân độ cứng của mô mềm-tăng tần số cộng hưởng Helmholtz do tăng trở kháng âm thanh. Co thắt phế quản, với những thay đổi về hình học ở các đường thở dẫn truyền nhỏ, làm giảm truyền năng lượng âm thanh đến các đường thở ngoại vi một phần do sự không khớp trở kháng giữa các nhánh đường thở. Thâm nhiễm phổi làm thay đổi đáng kể trường âm thanh cục bộ ở thùy bị ảnh hưởng. Việc tính toán sự khác biệt về âm thanh giữa các trường hợp khỏe mạnh so với bệnh lý có thể được sử dụng để nâng cao sự hiểu biết về những thay đổi rung-âm thanh có liên quan đến bệnh lý, và có khả năng cung cấp các công cụ cải tiến để chẩn đoán các bệnh phổi làm thay đổi riêng biệt về âm học của đường thở."} +{"idx": 1920133, "en": "A semi-automated method is described to range to vocalizing fin whales using the timing and amplitude of multipath arrivals measured on seafloor receivers. Calls are detected and multipath arrivals identified with a matched filter. Multipath times and relative amplitudes are predicted as a function of range by ray tracing. Because the direct and first water-column multiple arrivals are not always observed, different hypotheses for the observed arrival paths must be considered. For two arrivals, an amplitude threshold is used to determine if the first arrival is the direct path and if not, the call is disregarded as distant. When three or more arrivals are detected, three hypotheses for the paths of arrivals are considered; the solution is the hypothesis and range that minimizes the timing and optionally, amplitude ratio or absolute amplitude residual. The method is tested with data from two ocean bottom seismometers, one on the Juan de Fuca Ridge and the other in the Cascadia Basin. Solutions obtained by minimizing a combined residual from timing and an empirical absolute amplitude model extracted from the data yield reliable ranges up to 5 km at both sites, and are sufficient to estimate call density using point transect distance sampling.", "gemini": "Một phương pháp bán tự động được mô tả để đo phạm vi đến cá voi vây kêu bằng cách sử dụng thời gian và biên độ của các lần đến đa đường được đo trên các máy thu đáy biển. Các cuộc gọi được phát hiện và các lần đến đa đường được xác định bằng bộ lọc khớp. Thời gian đa đường và biên độ tương đối được dự đoán như một hàm của phạm vi bằng cách truy tìm tia. Vì không phải lúc nào cũng quan sát thấy các lần đến trực tiếp và đa lần đầu tiên của cột nước, nên phải xem xét các giả thuyết khác nhau cho các đường đến được quan sát. Đối với hai lần đến, ngưỡng biên độ được sử dụng để xác định xem lần đến đầu tiên có phải là đường trực tiếp hay không và nếu không, cuộc gọi sẽ bị coi là ở xa. Khi phát hiện ba lần đến trở lên, ba giả thuyết về đường đến được xem xét; giải pháp là giả thuyết và phạm vi giảm thiểu thời gian và tùy chọn, tỷ lệ biên độ hoặc biên độ tuyệt đối còn lại. Phương pháp này được thử nghiệm với dữ liệu từ hai máy đo địa chấn đáy đại dương, một ở Juan de Fuca Ridge và một ở Cascadia Basin. Các giải pháp thu được bằng cách giảm thiểu phần còn lại kết hợp từ thời gian và mô hình biên độ tuyệt đối theo kinh nghiệm được trích xuất từ dữ liệu cho kết quả phạm vi đáng tin cậy lên đến 5 km tại cả hai địa điểm và đủ để ước tính mật độ cuộc gọi bằng cách sử dụng mẫu khoảng cách tuyến điểm."} +{"idx": 1920134, "en": "A partial differential equation-constrained optimization approach is presented for reconstructing mechanical properties (e.g., elastic moduli). The proposed method is based on the minimization of an error in constitutive equations functional augmented with a least squares data misfit term referred to as MECE for \"modified error in constitutive equations.\" The main theme of this paper is to demonstrate several key strengths of the proposed method on experimental data. In addition, some illustrative examples are provided where the proposed method is compared with a common shear wave elastography (SWE) approach. To this end, both synthetic data, generated with transient finite element simulations, as well as ultrasonically tracked displacement data from an acoustic radiation force (ARF) experiment are used in a standard elasticity phantom. The results indicate that the MECE approach can produce accurate shear modulus reconstructions with significantly less bias than SWE.", "gemini": "Một phương pháp tối ưu hóa bị ràng buộc bởi phương trình vi phân riêng phần được trình bày để tái tạo các tính chất cơ học (ví dụ: mô đun đàn hồi). Phương pháp được đề xuất dựa trên việc giảm thiểu lỗi trong các phương trình cấu thành được tăng cường với một thuật ngữ không khớp dữ liệu bình phương nhỏ nhất được gọi là MECE cho \"lỗi đã sửa đổi trong các phương trình cấu thành\". Chủ đề chính của bài báo này là chứng minh một số điểm mạnh chính của phương pháp được đề xuất trên dữ liệu thực nghiệm. Ngoài ra, một số ví dụ minh họa được cung cấp, trong đó phương pháp được đề xuất được so sánh với phương pháp đàn hồi sóng cắt (SWE) thông thường. Để đạt được mục đích này, cả dữ liệu tổng hợp, được tạo bằng mô phỏng phần tử hữu hạn tạm thời, cũng như dữ liệu dịch chuyển được theo dõi bằng siêu âm từ một thí nghiệm lực bức xạ âm (ARF) đều được sử dụng trong một mô hình đàn hồi tiêu chuẩn. Kết quả cho thấy phương pháp MECE có thể tạo ra các bản tái tạo mô đun cắt chính xác với độ lệch nhỏ hơn đáng kể so với SWE."} +{"idx": 1920135, "en": "The lattice Boltzmann method (LBM) is emerging as a powerful engineering tool for aeroacoustic computations. However, the LBM has been shown to present accuracy and stability issues in the medium-low Mach number range, which is of interest for aeroacoustic applications. Several solutions have been proposed but are often too computationally expensive, do not retain the simplicity and the advantages typical of the LBM, or are not described well enough to be usable by the community due to proprietary software policies. An original regularized collision operator is proposed, based on the expansion of Hermite polynomials, that greatly improves the accuracy and stability of the LBM without significantly altering its algorithm. The regularized LBM can be easily coupled with both non-reflective boundary conditions and a multi-level grid strategy, essential ingredients for aeroacoustic simulations. Excellent agreement was found between this approach and both experimental and numerical data on two different benchmarks: the laminar, unsteady flow past a 2D cylinder and the 3D turbulent jet. Finally, most of the aeroacoustic computations with LBM have been done with commercial software, while here the entire theoretical framework is implemented using an open source library (palabos).", "gemini": "Phương pháp Boltzmann dạng lưới (LBM) đang nổi lên như một công cụ kỹ thuật mạnh mẽ cho các phép tính khí động lực học. Tuy nhiên, LBM đã được chứng minh là có vấn đề về độ chính xác và độ ổn định trong phạm vi số Mach trung bình thấp, điều này rất quan trọng đối với các ứng dụng khí động lực học. Một số giải pháp đã được đề xuất nhưng thường quá tốn kém về mặt tính toán, không giữ được sự đơn giản và những lợi thế điển hình của LBM hoặc không được mô tả đủ tốt để cộng đồng có thể sử dụng do các chính sách phần mềm độc quyền. Một toán tử va chạm chính quy ban đầu được đề xuất, dựa trên sự mở rộng của các đa thức Hermite, cải thiện đáng kể độ chính xác và độ ổn định của LBM mà không làm thay đổi đáng kể thuật toán của nó. LBM chính quy có thể dễ dàng kết hợp với cả điều kiện biên không phản xạ và chiến lược lưới đa cấp, các thành phần thiết yếu cho mô phỏng khí động lực học. Sự đồng ý tuyệt vời đã được tìm thấy giữa phương pháp tiếp cận này và cả dữ liệu thực nghiệm và số trên hai chuẩn mực khác nhau: dòng chảy không ổn định, tầng trên một xi lanh 2D và luồng phản lực nhiễu động 3D. Cuối cùng, hầu hết các phép tính khí động lực học với LBM đã được thực hiện bằng phần mềm thương mại, trong khi ở đây toàn bộ khuôn khổ lý thuyết được triển khai bằng cách sử dụng thư viện nguồn mở (palabos)."} +{"idx": 1920136, "en": "This work presents a lumped parameters equivalent model of condenser microphone based on analogies between acoustic, mechanical, fluidic, and electrical domains. Parameters of the model were determined mainly through analytical relations and/or finite element method (FEM) simulations. Special attention was paid to the air gap modeling and to the use of proper boundary condition. Corresponding lumped-parameters were obtained as results of FEM simulations. Because of its simplicity, the model allows a fast simulation and is readily usable for microphone design. This work shows the validation of the equivalent circuit on three real cases of capacitive microphones, including both traditional and Micro-Electro-Mechanical Systems structures. In all cases, it has been demonstrated that the sensitivity and other related data obtained from the equivalent circuit are in very good agreement with available measurement data.", "gemini": "Công trình này trình bày một mô hình tương đương các thông số tập hợp của micrô tụ điện dựa trên các phép tương tự giữa các miền âm học, cơ học, lưu chất và điện. Các thông số của mô hình chủ yếu được xác định thông qua các mối quan hệ phân tích và/hoặc mô phỏng phương pháp phần tử hữu hạn (FEM). Đặc biệt chú ý đến mô hình khe hở không khí và việc sử dụng điều kiện biên thích hợp. Các thông số tập hợp tương ứng thu được là kết quả của các mô phỏng FEM. Do tính đơn giản của nó, mô hình cho phép mô phỏng nhanh và dễ dàng sử dụng để thiết kế micrô. Công trình này cho thấy tính xác thực của mạch tương đương trên ba trường hợp thực tế của micrô điện dung, bao gồm cả cấu trúc Hệ thống cơ điện tử vi mô và truyền thống. Trong mọi trường hợp, đã chứng minh rằng độ nhạy và các dữ liệu liên quan khác thu được từ mạch tương đương rất phù hợp với dữ liệu đo lường có sẵn."} +{"idx": 1920137, "en": "It is predicted that Arctic Ocean acidity will increase during the next century as a result of carbon dioxide accumulation in the atmosphere and migration into ocean waters. This change has implications for sound transmission because low-pH seawater absorbs less sound than high-pH water. Altered pH will affect sound in the 0.3-10 kHz range if the criterion is met that absorption is the primary cause of attenuation, rather than the alternatives of loss in the ice or seabed. Recent work has exploited sound that meets the criterion, sound trapped in a Beaufort Sea duct composed of Pacific Winter Water underlying Pacific Summer Water. Arctic pH is expected to drop from 8.1 to 7.9 (approximately) over the next 30-50 yr, and effects of this chemical alteration on the intensity levels of this ducted sound, and on noise, are examined here. Sound near 900 Hz is predicted to undergo the greatest change, traveling up to 38% further. At ranges of 100-300 km, sound levels from a source in the duct may increase by 7 dB or more. Noise would also increase, but noise is ducted less efficiently, with the result that 1 kHz noise is predicted to rise approximately 0.5 dB.", "gemini": "Người ta dự đoán rằng độ axit của Bắc Băng Dương sẽ tăng trong thế kỷ tới do sự tích tụ carbon dioxide trong khí quyển và di chuyển vào các vùng nước đại dương. Sự thay đổi này có ý nghĩa đối với truyền âm vì nước biển có độ pH thấp hấp thụ ít âm thanh hơn nước có độ pH cao. Độ pH thay đổi sẽ ảnh hưởng đến âm thanh trong phạm vi 0,3-10 kHz nếu đáp ứng được tiêu chí là hấp thụ là nguyên nhân chính gây suy giảm, thay vì các phương án thay thế là mất mát trong băng hoặc đáy biển. Các công trình gần đây đã khai thác âm thanh đáp ứng tiêu chí, âm thanh bị giữ lại trong ống dẫn Biển Beaufort gồm Nước mùa đông Thái Bình Dương nằm bên dưới Nước mùa hè Thái Bình Dương. Độ pH của Bắc Cực dự kiến sẽ giảm từ 8,1 xuống còn 7,9 (xấp xỉ) trong 30-50 năm tới và các tác động của sự thay đổi hóa học này đối với mức cường độ của âm thanh được dẫn này và đối với tiếng ồn được kiểm tra ở đây. Âm thanh gần 900 Hz được dự đoán sẽ có sự thay đổi lớn nhất, di chuyển xa hơn tới 38%. Ở phạm vi 100-300 km, mức âm thanh từ nguồn trong ống dẫn có thể tăng thêm 7 dB trở lên. Tiếng ồn cũng sẽ tăng lên, nhưng tiếng ồn được dẫn ít hiệu quả hơn, kết quả là tiếng ồn 1 kHz được dự đoán sẽ tăng khoảng 0,5 dB."} +{"idx": 1920138, "en": "The theory and numerical simulations for the constant beam pattern (CBP) broadband transducer design are introduced. For a large spherical transducer, if the normal direction radial velocity distribution on the surface of the sphere transducer is a single Legendre polynomial, the far field angular beam pattern shows the same Legendre polynomial distribution due to a spherical Hankel function asymptotic approximation. Arbitrary radial velocity shading functions, including the equal sidelobe classic Dolph-Chebyshev shading, can be expanded by the Legendre series per Sturm-Liouville theory, such that the converging acoustic beam pattern displays the same angular shape as the original shading function itself for the broadband CBP transducer.", "gemini": "Lý thuyết và mô phỏng số cho thiết kế đầu dò băng thông rộng có kiểu chùm tia không đổi (CBP) được giới thiệu. Đối với đầu dò hình cầu lớn, nếu phân bố vận tốc hướng kính theo phương pháp thông thường trên bề mặt của đầu dò hình cầu là một đa thức Legendre đơn, thì kiểu chùm tia góc trường xa cho thấy cùng một phân bố đa thức Legendre do hàm Hankel hình cầu có dạng tiệm cận. Các hàm che bóng vận tốc hướng kính tùy ý, bao gồm cả che bóng Dolph-Chebyshev cổ điển có thùy phụ bằng nhau, có thể được mở rộng theo chuỗi Legendre theo lý thuyết Sturm-Liouville, sao cho kiểu chùm tia âm hội tụ hiển thị cùng hình dạng góc với chính hàm che bóng ban đầu cho đầu dò CBP băng thông rộng."} +{"idx": 1920139, "en": "Mechanical properties of the human vocal fold cover layer were experimentally investigated in uniaxial and biaxial tensile tests. The results showed a coupling effect between the stress conditions along the anterior-posterior and transverse directions, with vocal fold elongation increasing vocal fold stiffness along both directions, thus allowing more efficient control of the fundamental frequency of voice through vocal fold elongation. This study also shows that vocal folds were nearly isotropic at resting conditions, thus a tendency to vibrate with incomplete glottal closure, but became increasingly anisotropic with increasing vocal fold elongation.", "gemini": "Các tính chất cơ học của lớp phủ nếp gấp thanh quản của con người đã được nghiên cứu thực nghiệm trong các bài kiểm tra kéo đơn trục và lưỡng trục. Kết quả cho thấy có sự liên kết giữa các điều kiện ứng suất theo hướng trước-sau và hướng ngang, với độ giãn dài của nếp gấp thanh quản làm tăng độ cứng của nếp gấp thanh quản theo cả hai hướng, do đó cho phép kiểm soát hiệu quả hơn tần số cơ bản của giọng nói thông qua độ giãn dài của nếp gấp thanh quản. Nghiên cứu này cũng cho thấy rằng các nếp gấp thanh quản gần như đẳng hướng ở điều kiện nghỉ, do đó có xu hướng rung với sự đóng thanh môn không hoàn toàn, nhưng trở nên bất đẳng hướng ngày càng tăng khi độ giãn dài của nếp gấp thanh quản tăng lên."} +{"idx": 1920140, "en": "This research presents a parametric model order reduction approach for vibro-acoustic problems in the frequency domain of systems containing poroelastic materials (PEM). The method is applied to the Finite Element (FE) discretization of the weak u-p integral formulation based on the Biot-Allard theory and makes use of reduced basis (RB) methods typically employed for parametric problems. The parametric reduction is obtained rewriting the Biot-Allard FE equations for poroelastic materials using an affine representation of the frequency (therefore allowing for RB methods) and projecting the frequency-dependent PEM system on a global reduced order basis generated with the proper orthogonal decomposition instead of standard modal approaches. This has proven to be better suited to describe the nonlinear frequency dependence and the strong coupling introduced by damping. The methodology presented is tested on two three-dimensional systems: in the first experiment, the surface impedance of a PEM layer sample is calculated and compared with results of the literature; in the second, the reduced order model of a multilayer system coupled to an air cavity is assessed and the results are compared to those of the reference FE model.", "gemini": "Nghiên cứu này trình bày một phương pháp giảm bậc mô hình tham số cho các bài toán rung-âm trong miền tần số của các hệ thống chứa vật liệu xốp đàn hồi (PEM). Phương pháp được áp dụng cho phép phân tích phần tử hữu hạn (FE) của công thức tích phân u-p yếu dựa trên lý thuyết Biot-Allard và sử dụng các phương pháp cơ sở thu gọn (RB) thường được sử dụng cho các bài toán tham số. Việc giảm tham số thu được bằng cách viết lại các phương trình FE Biot-Allard cho vật liệu xốp đàn hồi bằng cách sử dụng biểu diễn affine của tần số (do đó cho phép sử dụng các phương pháp RB) và chiếu hệ thống PEM phụ thuộc tần số trên cơ sở thu gọn toàn cục được tạo bằng phân hủy trực giao thích hợp thay vì các phương pháp mô thức chuẩn. Điều này đã được chứng minh là phù hợp hơn để mô tả sự phụ thuộc tần số phi tuyến tính và sự liên kết chặt chẽ do giảm chấn gây ra. Phương pháp được trình bày được thử nghiệm trên hai hệ thống ba chiều: trong thí nghiệm đầu tiên, trở kháng bề mặt của một mẫu lớp PEM được tính toán và so sánh với kết quả của tài liệu; trong thí nghiệm thứ hai, mô hình bậc thu gọn của một hệ thống nhiều lớp được ghép với một khoang khí được đánh giá và kết quả được so sánh với kết quả của mô hình FE tham chiếu."} +{"idx": 1920141, "en": "High order Lamb waves are investigated for the effects of stress on both symmetrical and anti-symmetrical modes in an aluminum plate for wave propagation and load parallel. Data are compared with those for the case of load and measurement axis perpendicular. It is the S1 mode which exhibits significantly higher sensitivity to stress than other Lamb modes. For aluminum the use of the S1 mode for stress measurement is found to be about six times more sensitive, than bulk waves, for the load-measurement axes parallel case and this compares with about ten times for the case of load-measurement axes perpendicular.", "gemini": "Các sóng Lamb bậc cao được nghiên cứu để tìm hiểu tác động của ứng suất lên cả chế độ đối xứng và phản đối xứng trong một tấm nhôm để truyền sóng và tải song song. Dữ liệu được so sánh với dữ liệu của trường hợp trục tải và đo vuông góc. Đó là chế độ S1 biểu hiện độ nhạy cao hơn đáng kể đối với ứng suất so với các chế độ Lamb khác. Đối với nhôm, việc sử dụng chế độ S1 để đo ứng suất được phát hiện nhạy hơn khoảng sáu lần so với sóng khối, đối với trường hợp trục tải-đo song song và điều này tương đương với khoảng mười lần đối với trường hợp trục tải-đo vuông góc."} +{"idx": 1920142, "en": "Small aperture arrays (size less than a wavelength) can be used for passive angle of arrival (AOA) estimation of both broadband and narrowband signals in the frequency domain. Phase differences across the array are measured in the frequency domain and can be spectrally averaged if desired for stationary source AOA and frequencies. The array is capable of measuring multiple target AOAs so long as each target produces unique frequencies or radiates in unique frequency bandwidths. Broadband and narrowband signals from targets can be collected from the fast Fourier transform (FFT) bins and summed by arrival angle in elevation and azimuth using a bearing histogram where the amplitude is displayed in dB. This provides a very useful and intuitive display of received signal levels. The Cramer-Rao lower bound (CRLB) for bearing accuracy is presented as a function of frequency, aperture, and signal-to-noise ratio (SNR). The small aperture background noise includes any other coherent or incoherent signal other than the source of interest. Analysis of phase calibration requirements, nonstationary frequencies, and channel cross-talk are also presented, as well as a technique for generating broadband source signals with the fractional delays needed to simulate the correct AOA.", "gemini": "Có thể sử dụng các mảng khẩu độ nhỏ (kích thước nhỏ hơn bước sóng) để ước tính góc đến thụ động (AOA) của cả tín hiệu băng thông rộng và băng hẹp trong miền tần số. Các chênh lệch pha trên mảng được đo trong miền tần số và có thể được trung bình theo phổ nếu muốn đối với AOA nguồn tĩnh và tần số. Mảng có khả năng đo nhiều AOA mục tiêu miễn là mỗi mục tiêu tạo ra các tần số duy nhất hoặc phát ra trong các băng thông tần số duy nhất. Các tín hiệu băng thông rộng và băng hẹp từ các mục tiêu có thể được thu thập từ các thùng biến đổi Fourier nhanh (FFT) và được tổng hợp theo góc đến theo độ cao và phương vị bằng cách sử dụng biểu đồ ổ trục trong đó biên độ được hiển thị ở dB. Điều này cung cấp một màn hình rất hữu ích và trực quan về mức tín hiệu được nhận. Giới hạn dưới của Cramer-Rao (CRLB) đối với độ chính xác của ổ trục được trình bày như một hàm của tần số, khẩu độ và tỷ lệ tín hiệu trên nhiễu (SNR). Tiếng ồn nền khẩu độ nhỏ bao gồm bất kỳ tín hiệu đồng pha hoặc không đồng pha nào khác ngoài nguồn được quan tâm. Phân tích các yêu cầu hiệu chuẩn pha, tần số không dừng và nhiễu xuyên kênh cũng được trình bày, cũng như một kỹ thuật để tạo ra các tín hiệu nguồn băng thông rộng với các độ trễ phân số cần thiết để mô phỏng AOA chính xác."} +{"idx": 1920143, "en": "Although stiffness conditions in the multi-layered vocal folds are generally considered to have a large impact on voice production, their specific role in controlling vocal fold vibration and voice acoustics is unclear. Using a three-dimensional body-cover continuum model of phonation, this study shows that changes in vocal fold stiffness have a large effect on F0 and the means and amplitudes of the glottal area and flow rate. However, varying vocal fold stiffness, particularly along the anterior-posterior direction, has a much smaller effect on the closed quotient, vertical phase difference, and the spectral shape of the output acoustics, which are more effectively controlled by changes in the vertical thickness of the medial surface. These results suggest that although changes in vocal fold stiffness are often correlated with production of different voice types, there is no direct cause-effect relation between vocal fold stiffness and voice types, and the correlation may simply result from the fact that both vocal fold stiffness and geometry are regulated by the same set of laryngeal muscles. These results also suggest the possibility of developing reduced-order models of phonation in which the vocal fold is simplified to a one-layer structure.", "gemini": "Mặc dù các điều kiện độ cứng ở các nếp gấp thanh quản nhiều lớp thường được coi là có tác động lớn đến việc phát ra giọng nói, nhưng vai trò cụ thể của chúng trong việc kiểm soát độ rung của nếp gấp thanh quản và âm học của giọng nói vẫn chưa rõ ràng. Sử dụng mô hình liên tục phủ cơ thể ba chiều của quá trình phát âm, nghiên cứu này cho thấy những thay đổi về độ cứng của nếp gấp thanh quản có tác động lớn đến F0 và phương tiện và biên độ của vùng thanh môn và lưu lượng dòng chảy. Tuy nhiên, độ cứng của nếp gấp thanh quản khác nhau, đặc biệt là theo hướng trước sau, có tác động nhỏ hơn nhiều đến thương số đóng, chênh lệch pha theo chiều dọc và hình dạng phổ của âm học đầu ra, vốn được kiểm soát hiệu quả hơn bởi những thay đổi về độ dày theo chiều dọc của bề mặt trung gian. Những kết quả này cho thấy rằng mặc dù những thay đổi về độ cứng của nếp gấp thanh quản thường có liên quan đến việc sản xuất các loại giọng nói khác nhau, nhưng không có mối quan hệ nhân quả trực tiếp giữa độ cứng của nếp gấp thanh quản và các loại giọng nói và mối tương quan này có thể chỉ đơn giản là kết quả của thực tế là cả độ cứng và hình dạng của nếp gấp thanh quản đều được điều chỉnh bởi cùng một tập hợp các cơ thanh quản. Những kết quả này cũng gợi ý khả năng phát triển các mô hình phát âm bậc giảm trong đó nếp gấp thanh quản được đơn giản hóa thành một cấu trúc một lớp."} diff --git a/retranslated_vi_pubmed/chunk_192/error.json b/retranslated_vi_pubmed/chunk_192/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_192/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_193/completed.json b/retranslated_vi_pubmed/chunk_193/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..a966dad3d05847794e41b1fca4efb89b182ee92e --- /dev/null +++ b/retranslated_vi_pubmed/chunk_193/completed.json @@ -0,0 +1,146 @@ +[ + 1930000, + 1930001, + 1930002, + 1930003, + 1930004, + 1930005, + 1930006, + 1930007, + 1930008, + 1930009, + 1930010, + 1930011, + 1930012, + 1930013, + 1930014, + 1930015, + 1930016, + 1930017, + 1930018, + 1930019, + 1930020, + 1930021, + 1930022, + 1930023, + 1930024, + 1930025, + 1930026, + 1930027, + 1930028, + 1930029, + 1930030, + 1930031, + 1930032, + 1930033, + 1930034, + 1930035, + 1930036, + 1930037, + 1930038, + 1930039, + 1930040, + 1930041, + 1930042, + 1930043, + 1930044, + 1930045, + 1930046, + 1930047, + 1930048, + 1930049, + 1930050, + 1930051, + 1930052, + 1930053, + 1930054, + 1930055, + 1930056, + 1930057, + 1930058, + 1930059, + 1930060, + 1930061, + 1930062, + 1930063, + 1930064, + 1930065, + 1930066, + 1930067, + 1930068, + 1930069, + 1930070, + 1930071, + 1930072, + 1930073, + 1930074, + 1930075, + 1930076, + 1930077, + 1930078, + 1930079, + 1930080, + 1930081, + 1930082, + 1930083, + 1930084, + 1930085, + 1930086, + 1930087, + 1930088, + 1930089, + 1930090, + 1930091, + 1930092, + 1930093, + 1930094, + 1930095, + 1930096, + 1930097, + 1930098, + 1930099, + 1930100, + 1930101, + 1930102, + 1930103, + 1930104, + 1930105, + 1930106, + 1930107, + 1930108, + 1930109, + 1930110, + 1930111, + 1930112, + 1930113, + 1930114, + 1930115, + 1930116, + 1930117, + 1930118, + 1930119, + 1930120, + 1930121, + 1930122, + 1930123, + 1930124, + 1930125, + 1930126, + 1930127, + 1930128, + 1930129, + 1930130, + 1930131, + 1930132, + 1930133, + 1930134, + 1930135, + 1930136, + 1930137, + 1930138, + 1930139, + 1930140, + 1930141, + 1930142, + 1930143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_193/data.jsonl b/retranslated_vi_pubmed/chunk_193/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..3ea16f98bb71a0bffb957303700c60310618eba2 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_193/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1930000, "en": "Ecological stability is the central framework to understand an ecosystem's ability to absorb or recover from environmental change. Recent modelling and conceptual work suggests that stability is a multidimensional construct comprising different response aspects. Using two freshwater mesocosm experiments as case studies, we show how the response to single perturbations can be decomposed in different stability aspects (resistance, resilience, recovery, temporal stability) for both ecosystem functions and community composition. We find that extended community recovery is tightly connected to a nearly complete recovery of the function (biomass production), whereas systems with incomplete recovery of the species composition ranged widely in their biomass compared to controls. Moreover, recovery was most complete when either resistance or resilience was high, the latter associated with low temporal stability around the recovery trend. In summary, no single aspect of stability was sufficient to reflect the overall stability of the system.", "gemini": "Tính ổn định sinh thái là khuôn khổ trung tâm để hiểu khả năng hấp thụ hoặc phục hồi của một hệ sinh thái sau biến đổi môi trường. Mô hình hóa và công trình khái niệm gần đây cho thấy rằng tính ổn định là một cấu trúc đa chiều bao gồm các khía cạnh phản ứng khác nhau. Sử dụng hai thí nghiệm mesocosm nước ngọt làm nghiên cứu trường hợp, chúng tôi chỉ ra cách phản ứng với các nhiễu động đơn lẻ có thể được phân hủy thành các khía cạnh ổn định khác nhau (khả năng chống chịu, khả năng phục hồi, khả năng phục hồi, tính ổn định tạm thời) đối với cả chức năng hệ sinh thái và thành phần cộng đồng. Chúng tôi thấy rằng sự phục hồi của cộng đồng mở rộng có liên quan chặt chẽ đến sự phục hồi gần như hoàn toàn của chức năng (sản xuất sinh khối), trong khi các hệ thống có sự phục hồi không hoàn toàn về thành phần loài có phạm vi sinh khối rộng so với các đối chứng. Hơn nữa, sự phục hồi hoàn thiện nhất khi khả năng chống chịu hoặc khả năng phục hồi cao, khả năng phục hồi sau này liên quan đến tính ổn định tạm thời thấp xung quanh xu hướng phục hồi. Tóm lại, không có khía cạnh ổn định nào là đủ để phản ánh tính ổn định tổng thể của hệ thống."} +{"idx": 1930001, "en": "Previous studies have shown that the mammalian δ-aminolevulinic acid dehydratase (δ-ALAD) is inhibited by selenides and selenoxides, which can involve thiol oxidation. However, the precise molecular interaction of selenides and selenoxides with the active center of the enzyme is unknown. Here, we try to explain the interaction of selenides and the respective selenoxides with human δ-ALAD by in silico molecular docking. The in silico data indicated that Se atoms of selenoxides have higher electrophilic character than their respective selenides. Further, the presence of oxygen increased the interaction of selenoxides with the δ-ALAD active site by O…Zn coordination. The interaction of S atom from Cys124 with the Se atom indicated the importance of the nucleophilic attack of the enzyme thiolate to the organoselenium molecules. These observations help us to understand the interaction of target proteins with organoselenium compounds.", "gemini": "Các nghiên cứu trước đây đã chỉ ra rằng δ-aminolevulinic acid dehydratase (δ-ALAD) của động vật có vú bị ức chế bởi selenide và selenoxide, có thể liên quan đến quá trình oxy hóa thiol. Tuy nhiên, tương tác phân tử chính xác của selenide và selenoxide với trung tâm hoạt động của enzyme vẫn chưa được biết rõ. Ở đây, chúng tôi cố gắng giải thích sự tương tác của selenide và selenoxide tương ứng với δ-ALAD của người bằng cách ghép nối phân tử in silico. Dữ liệu in silico chỉ ra rằng các nguyên tử Se của selenoxide có tính ái điện tử cao hơn so với selenide tương ứng của chúng. Hơn nữa, sự có mặt của oxy làm tăng sự tương tác của selenoxide với vị trí hoạt động của δ-ALAD bằng phối hợp O…Zn. Sự tương tác của nguyên tử S từ Cys124 với nguyên tử Se chỉ ra tầm quan trọng của sự tấn công ái nhân của thiolate enzyme đối với các phân tử organoselenium. Những quan sát này giúp chúng tôi hiểu được sự tương tác của các protein đích với các hợp chất organoselenium."} +{"idx": 1930002, "en": "Essentials Specific reversal agents for managing severe factor Xa inhibitor-associated bleeding are lacking. We assessed 4-factor-prothrombin complex concentrate (4F-PCC) and tranexamic acid (TXA). 4F-PCC, but not TXA, reduced the prothrombin time and increased endogenous thrombin potential. These agents may be viable options for reversal of therapeutic doses of rivaroxaban. SUMMARY Background Oral activated factor X inhibitors such as rivaroxaban are widely used, but specific reversal agents are lacking. Although four-factor prothrombin complex concentrate (4F-PCC) and tranexamic acid (TXA) are sometimes used to manage serious bleeding, their efficacy is unknown. Prior studies in healthy subjects taking rivaroxaban revealed that 4F-PCC partially reverses the prolonged prothrombin time (PT), and fully restores the endogenous thrombin potential (ETP). The effect of TXA has not been evaluated. Methods In this double-blind, parallel-group study, 147 healthy volunteers given rivaroxaban 20 mg twice daily for 3 days were randomized after their morning dose on day 4 to receive intravenous 4F-PCC (50 IU kg-1 ), TXA (1.0 g), or saline. Standardized punch biopsies were performed at baseline and after 4F-PCC, TXA or saline administration. Reversal was assessed by measuring bleeding duration and bleeding volume at biopsy sites, and by determining the PT and ETP. Results As compared with saline, 4F-PCC partially reversed the PT and completely reversed the ETP, whereas TXA had no effect. Neither 4F-PCC nor TXA reduced bleeding duration or volume. All treatments were well tolerated, with no recorded adverse events. Conclusions Although 4F-PCC reduced the PT and increased the ETP in volunteers given supratherapeutic doses of rivaroxaban, neither 4F-PCC nor TXA influenced punch biopsy bleeding.", "gemini": "Thiết yếu Thiếu các tác nhân đảo ngược đặc hiệu để kiểm soát tình trạng chảy máu liên quan đến chất ức chế yếu tố Xa nghiêm trọng. Chúng tôi đã đánh giá 4 yếu tố cô đặc phức hợp prothrombin (4F-PCC) và axit tranexamic (TXA). 4F-PCC, nhưng không phải TXA, làm giảm thời gian prothrombin và tăng tiềm năng thrombin nội sinh. Những tác nhân này có thể là các lựa chọn khả thi để đảo ngược liều điều trị rivaroxaban. TÓM TẮT Bối cảnh Các chất ức chế yếu tố X hoạt hóa đường uống như rivaroxaban được sử dụng rộng rãi, nhưng thiếu các tác nhân đảo ngược đặc hiệu. Mặc dù đôi khi người ta sử dụng phức hợp prothrombin bốn yếu tố cô đặc (4F-PCC) và axit tranexamic (TXA) để kiểm soát tình trạng chảy máu nghiêm trọng, nhưng hiệu quả của chúng vẫn chưa được biết. Các nghiên cứu trước đây ở những người khỏe mạnh dùng rivaroxaban cho thấy 4F-PCC đảo ngược một phần thời gian prothrombin kéo dài (PT) và khôi phục hoàn toàn tiềm năng thrombin nội sinh (ETP). Hiệu quả của TXA vẫn chưa được đánh giá. Phương pháp Trong nghiên cứu song song, mù đôi này, 147 tình nguyện viên khỏe mạnh dùng rivaroxaban 20 mg hai lần một ngày trong 3 ngày đã được phân ngẫu nhiên sau liều dùng buổi sáng của họ vào ngày thứ 4 để tiêm tĩnh mạch 4F-PCC (50 IU kg-1), TXA (1,0 g) hoặc nước muối. Sinh thiết đục lỗ chuẩn hóa được thực hiện tại thời điểm ban đầu và sau khi dùng 4F-PCC, TXA hoặc nước muối. Sự đảo ngược được đánh giá bằng cách đo thời gian chảy máu và thể tích chảy máu tại các vị trí sinh thiết và bằng cách xác định PT và ETP. Kết quả So với nước muối, 4F-PCC đảo ngược một phần PT và đảo ngược hoàn toàn ETP, trong khi TXA không có tác dụng. Cả 4F-PCC và TXA đều không làm giảm thời gian hoặc thể tích chảy máu. Tất cả các phương pháp điều trị đều được dung nạp tốt, không ghi nhận tác dụng phụ nào. Kết luận Mặc dù 4F-PCC làm giảm PT và tăng ETP ở những tình nguyện viên dùng liều rivaroxaban cao hơn mức điều trị, nhưng cả 4F-PCC và TXA đều không ảnh hưởng đến chảy máu sinh thiết đục lỗ."} +{"idx": 1930003, "en": "OBJECTIVES To analyze the impact of actions organized by a quality of care improvement team on the use of sedatives when treating wounds in children under the age of 5 years. MATERIAL AND METHODS Quasiexperimental pre/post study enrolling children under the age of 5 years brought to a pediatric emergency department with wounds requiring surgical repair with suturing. A team to promote the use of sedation in such minor procedures in these children was established. The team organized the following interventions: training workshops, development and circulation of a sedation protocol, and establishment of a computerized alert. The first analysis of results was done at 2 months and the second at 9 months. The quality of care indicators, the use of sedatives while wounds were treated in children, was analized in 2 age groups: (under the age of 2 years and between 2 and 5 years) and results were compared with the preintervention phase. RESULTS A total of 22 958 emergencies were registered in children under 5 years old; 548 (2.4%) involved uncomplicated wounds. Of the 548 patients, 350 (63.8%) required surgical repair, 75 of them (21.4%) in children under the age of 2 years. Ten percent of these children had received a sedative in the period before the team's intervention; 22% had been sedated at the 2-month analysis and 31.4% at 9 months (P<.01). For children between 2 and 5 years old, the percentages were 4.4% (pre-intervention), 10% (2 months), and 25% (9 months) (P<.01). Eighty-two percent of the families and 69% of the physicians thought that anxiety was adequately controlled. CONCLUSION Actions designed by a multidisciplinary quality of care team are effective for increasing the use of sedatives while wounds are treated in children under the age of 5 years.", "gemini": "MỤC TIÊU Phân tích tác động của các hành động do nhóm cải tiến chất lượng chăm sóc tổ chức đối với việc sử dụng thuốc an thần khi điều trị vết thương ở trẻ em dưới 5 tuổi. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu tiền/hậu bán thực nghiệm tuyển chọn trẻ em dưới 5 tuổi đưa đến khoa cấp cứu nhi với vết thương cần phẫu thuật khâu. Một nhóm thúc đẩy việc sử dụng thuốc an thần trong các thủ thuật nhỏ này ở những trẻ em này đã được thành lập. Nhóm đã tổ chức các can thiệp sau: hội thảo đào tạo, phát triển và lưu hành một giao thức an thần và thiết lập cảnh báo bằng máy tính. Phân tích kết quả đầu tiên được thực hiện sau 2 tháng và lần thứ hai sau 9 tháng. Các chỉ số chất lượng chăm sóc, việc sử dụng thuốc an thần trong khi điều trị vết thương ở trẻ em, được phân tích ở 2 nhóm tuổi: (dưới 2 tuổi và từ 2 đến 5 tuổi) và kết quả được so sánh với giai đoạn trước can thiệp. KẾT QUẢ Tổng cộng có 22.958 trường hợp cấp cứu được ghi nhận ở trẻ em dưới 5 tuổi; 548 (2,4%) liên quan đến vết thương không biến chứng. Trong số 548 bệnh nhân, 350 (63,8%) cần phẫu thuật, 75 trong số đó (21,4%) ở trẻ dưới 2 tuổi. Mười phần trăm trong số những trẻ em này đã được dùng thuốc an thần trong giai đoạn trước khi nhóm can thiệp; 22% đã được dùng thuốc an thần khi phân tích sau 2 tháng và 31,4% sau 9 tháng (P <.01). Đối với trẻ em từ 2 đến 5 tuổi, tỷ lệ phần trăm là 4,4% (trước can thiệp), 10% (2 tháng) và 25% (9 tháng) (P <.01). Tám mươi hai phần trăm các gia đình và 69% các bác sĩ cho rằng tình trạng lo lắng được kiểm soát đầy đủ. KẾT LUẬN Các hành động do nhóm chất lượng chăm sóc đa ngành thiết kế có hiệu quả trong việc tăng cường sử dụng thuốc an thần trong khi điều trị vết thương ở trẻ em dưới 5 tuổi."} +{"idx": 1930004, "en": "OBJECTIVES Residents of long-term care facilities (LTCFs) are at risk for methicillin-resistant Staphylococcus aureus (MRSA) colonization because of age-related illnesses and high rates of hospital use, in particular, of visits to the emergency department (ED). We aimed to determine the prevalence of and risk factors for MRSA colonization in LTCF residents. MATERIAL AND METHODS A descriptive cross-sectional study was carried out in 2014 (April–June). LTCF residents in the northern part of the island of Tenerife were eligible for enrollment. We collected clinical and epidemiologic data and took nasal swabs for culture (chromID MRSA agar) to screen for MRSA colonization. Molecular typing was established by pulsedfield gel electrophoresis. MRSA colonization was the dependent variable in logistic regression analysis. RESULTS A total of 624 residents were enrolled. MRSA was detected in 25.8%. Sixty-four of the residents had received care in a hospital ED in the 3 months prior to enrollment. Multivariant regression analysis detected 2 risk factors for MRSA colonization: hospital ED care in the last 3 months (odds ratio [OR], 2.05; 95% CI, 1.29–3.26; P=.002) and the presence of skin lesions (OR, 1.65; 95% CI, 1.11–2.44); P=.013). The health-care-associated, ST5 MRSA-IVa, was the most prevalent (75.8%). CONCLUSION LTCF residents in our area are a significant reservoir of MRSA colonization. Hospital ED care was a predictor of MRSA colonization. We believe that stronger measures to prevent cross-contamination of multidrug resistant microorganisms must be implemented, along with active vigilance systems to detect MRSA in hospitals.", "gemini": "MỤC TIÊU Những cư dân của các cơ sở chăm sóc dài hạn (LTCF) có nguy cơ nhiễm tụ cầu vàng kháng methicillin (MRSA) do các bệnh liên quan đến tuổi tác và tỷ lệ sử dụng bệnh viện cao, đặc biệt là các lần đến khoa cấp cứu (ED). Chúng tôi nhằm xác định tỷ lệ lưu hành và các yếu tố nguy cơ nhiễm MRSA ở cư dân LTCF. VẬT LIỆU VÀ PHƯƠNG PHÁP Một nghiên cứu cắt ngang mô tả đã được thực hiện vào năm 2014 (tháng 4 - tháng 6). Những cư dân của LTCF ở phía bắc đảo Tenerife đủ điều kiện để đăng ký. Chúng tôi đã thu thập dữ liệu lâm sàng và dịch tễ học và lấy dịch mũi để nuôi cấy (agar chromID MRSA) để sàng lọc nhiễm MRSA. Kiểu phân tử được thiết lập bằng điện di gel xung. Nhiễm MRSA là biến phụ thuộc trong phân tích hồi quy logistic. KẾT QUẢ Tổng cộng có 624 cư dân đã được đăng ký. MRSA được phát hiện ở 25,8%. Sáu mươi bốn cư dân đã được chăm sóc tại khoa cấp cứu của bệnh viện trong 3 tháng trước khi đăng ký. Phân tích hồi quy đa biến phát hiện ra 2 yếu tố nguy cơ nhiễm MRSA: chăm sóc tại khoa cấp cứu của bệnh viện trong 3 tháng gần nhất (tỷ lệ cược [OR], 2,05; KTCB 95%, 1,29–3,26; P = 0,002) và có tổn thương da (OR, 1,65; KTCB 95%, 1,11–2,44); P = 0,013). MRSA-IVa liên quan đến chăm sóc sức khỏe, ST5, là loại lưu hành nhiều nhất (75,8%). KẾT LUẬN Những cư dân của LTCF trong khu vực của chúng tôi là một ổ chứa đáng kể của nhiễm MRSA. Chăm sóc tại khoa cấp cứu của bệnh viện là một yếu tố dự báo nhiễm MRSA. Chúng tôi tin rằng các biện pháp mạnh mẽ hơn để ngăn ngừa lây nhiễm chéo các vi sinh vật đa kháng thuốc phải được thực hiện, cùng với các hệ thống cảnh giác chủ động để phát hiện MRSA trong các bệnh viện."} +{"idx": 1930005, "en": "OBJECTIVES To establish the negative appendectomy rate (NAR) after patients with acute abdomen were evaluated with the Alvarado score and compare it to the NAR in patients evaluated with abdominal ultrasound. MATERIAL AND METHODS Cross-sectional, retrospective, descriptive study in patients who underwent emergency surgery for suspected acute appendicitis in a tertiary-care hospital over a period of 1 year. RESULTS A total of 225 patients were included. The NAR was 7.11% for the series. An Alvarado score of 5 or more had an odds ratio (OR) of 7.46 (P=.0002) for acute appendicitis; sensitivity was 94.2%. Ultrasound findings consistent with acute appendicitis had an OR of 3.58 (P=.0125) for the diagnosis; the NAR was 5%. CONCLUSION The high sensitivity of an Alvarado score of 5 or more supports using this tool to evaluate all patients who come to the emergency department with pain in the right iliac fossa. With scores of 7 or more the NAR falls to 3%. Abdominal ultrasound is a rapid, inexpensive diagnostic procedure associated with a low NAR.", "gemini": "MỤC TIÊU Thiết lập tỷ lệ cắt ruột thừa âm tính (NAR) sau khi những bệnh nhân bị đau bụng cấp được đánh giá bằng điểm Alvarado và so sánh với NAR ở những bệnh nhân được đánh giá bằng siêu âm ổ bụng. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu mô tả, hồi cứu, cắt ngang ở những bệnh nhân đã phẫu thuật cấp cứu vì nghi ngờ viêm ruột thừa cấp tại một bệnh viện chăm sóc sức khỏe ba cấp trong thời gian 1 năm. KẾT QUẢ Tổng cộng có 225 bệnh nhân được đưa vào. NAR là 7,11% cho toàn bộ ca bệnh. Điểm Alvarado từ 5 trở lên có tỷ lệ cược (OR) là 7,46 (P = 0,0002) đối với viêm ruột thừa cấp; độ nhạy là 94,2%. Các phát hiện siêu âm phù hợp với viêm ruột thừa cấp có OR là 3,58 (P = 0,0125) đối với chẩn đoán; NAR là 5%. KẾT LUẬN Độ nhạy cao của điểm Alvarado từ 5 trở lên hỗ trợ việc sử dụng công cụ này để đánh giá tất cả những bệnh nhân đến khoa cấp cứu vì đau ở hố chậu phải. Với điểm từ 7 trở lên, NAR giảm xuống còn 3%. Siêu âm ổ bụng là một thủ thuật chẩn đoán nhanh chóng, không tốn kém, có liên quan đến NAR thấp."} +{"idx": 1930006, "en": "Persons with diabetes make up a large percentage of patients attended in the emergency department. Most will be discharged, but patients who remain under observation in wards managed by the emergency department or who wait are waiting to be admitted to a conventional ward must receive appropriate, protocol-guided treatment for their diabetes. Situations of hyper- or hypoglycemia must be avoided because both worsen prognosis. Emergency physicians must correctly and efficiently prevent, diagnose, and manage acute metabolic complications of diabetes such as simple hyperglycemia, diabetic ketoacidosis, and hyperosmolar hyperglycemic state. They must also be ready to prescribe and properly administer intravenous insulin to critically ill patients. Hyperglycemia induced by treatment with steroids deserves special mention. If this complication develops, the hyperglycemia is intense, influenced by increased insulin resistance and gluconeogenesis in the liver. Thus, it usually appears after meals and is dependent on steroid dose, duration of treatment, and individual predisposition. The recommendations in this paper elaborated by consensus of the Spanish Society of Emergency Medicine (SEMES) experts, are the first to be written specifically for use in emergency departments in Spain. They give a detailed, in-depth overview of emergencies related to diabetes and diabetic complications.", "gemini": "Những người bị tiểu đường chiếm tỷ lệ lớn bệnh nhân được chăm sóc tại khoa cấp cứu. Hầu hết sẽ được xuất viện, nhưng những bệnh nhân vẫn được theo dõi tại các khoa do khoa cấp cứu quản lý hoặc những người đang chờ nhập viện vào một khoa thông thường phải được điều trị thích hợp theo hướng dẫn của phác đồ điều trị bệnh tiểu đường của họ. Cần tránh tình trạng tăng hoặc hạ đường huyết vì cả hai đều làm cho tiên lượng bệnh trở nên tồi tệ hơn. Các bác sĩ cấp cứu phải ngăn ngừa, chẩn đoán và xử trí chính xác và hiệu quả các biến chứng chuyển hóa cấp tính của bệnh tiểu đường như tăng đường huyết đơn giản, nhiễm toan ceton do tiểu đường và tình trạng tăng thẩm thấu tăng đường huyết. Họ cũng phải sẵn sàng kê đơn và tiêm tĩnh mạch insulin đúng cách cho những bệnh nhân bị bệnh nặng. Tăng đường huyết do điều trị bằng steroid cần được đề cập đặc biệt. Nếu biến chứng này phát triển, tình trạng tăng đường huyết sẽ trở nên dữ dội, bị ảnh hưởng bởi tình trạng kháng insulin tăng và tăng tân đường ở gan. Do đó, tình trạng này thường xuất hiện sau bữa ăn và phụ thuộc vào liều steroid, thời gian điều trị và cơ địa của từng người. Các khuyến nghị trong bài báo này được các chuyên gia của Hiệp hội Y học Cấp cứu Tây Ban Nha (SEMES) nhất trí đưa ra, là những khuyến nghị đầu tiên được viết riêng để sử dụng trong các khoa cấp cứu tại Tây Ban Nha. Các khuyến nghị này cung cấp một cái nhìn tổng quan chi tiết và sâu sắc về các trường hợp cấp cứu liên quan đến bệnh tiểu đường và các biến chứng do bệnh tiểu đường."} +{"idx": 1930007, "en": "OBJECTIVES To study the prognostic role of elevated troponin I levels in patients discharged home directly from a hospital emergency department. MATERIAL AND METHODS Observational study of a retrospective cohort of all patients attended for any emergency for whom troponin I tests were ordered and who were discharged home directly from our hospital emergency department between January and December 2012. We collected demographic information, medical histories, symptoms related to the acute coronary event, and diagnosis on discharge. The main outcome was all-cause mortality in the year following discharge. RESULTS A total of 1381 patients discharged home directly from the emergency department were studied; 1192 (86.3%) had normal troponin I results and 189 (13.7%) had elevated levels. On multivariate analysis, troponin I elevation emerged as an independent risk factor for death within a year of discharge (hazard ratio, 2.41; 95% CI, 1.40-4.22; P<.01). CONCLUSION A raised troponin I level is an independent predictor of 1-year mortality in patients for whom this test is ordered at least once and who are discharged home directly from a hospital emergency service.", "gemini": "MỤC TIÊU Nghiên cứu vai trò tiên lượng của nồng độ troponin I tăng cao ở những bệnh nhân được xuất viện về nhà trực tiếp từ khoa cấp cứu của bệnh viện. VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu quan sát một nhóm bệnh nhân hồi cứu gồm tất cả những bệnh nhân đến khám cấp cứu vì bất kỳ lý do gì mà được chỉ định xét nghiệm troponin I và được xuất viện về nhà trực tiếp từ khoa cấp cứu của bệnh viện chúng tôi trong khoảng thời gian từ tháng 1 đến tháng 12 năm 2012. Chúng tôi đã thu thập thông tin nhân khẩu học, tiền sử bệnh, các triệu chứng liên quan đến sự kiện tim mạch cấp tính và chẩn đoán khi xuất viện. Kết cục chính là tỷ lệ tử vong toàn nguyên nhân trong năm sau khi xuất viện. KẾT QUẢ Tổng cộng có 1381 bệnh nhân được xuất viện về nhà trực tiếp từ khoa cấp cứu được nghiên cứu; 1192 (86,3%) có kết quả troponin I bình thường và 189 (13,7%) có nồng độ tăng cao. Khi phân tích đa biến, nồng độ troponin I tăng cao nổi lên như một yếu tố nguy cơ độc lập gây tử vong trong vòng một năm sau khi xuất viện (tỷ lệ nguy cơ, 2,41; KTC 95%, 1,40-4,22; P<.01). KẾT LUẬN Nồng độ troponin I tăng cao là một yếu tố dự báo độc lập về tỷ lệ tử vong sau 1 năm ở những bệnh nhân được chỉ định xét nghiệm này ít nhất một lần và được xuất viện về nhà trực tiếp từ khoa cấp cứu của bệnh viện."} +{"idx": 1930008, "en": "OBJECTIVES To assess the efficacy of a nitrous oxide and oxygen mixture (N2O/O2 50/50) for reducing pain and increasing satisfaction in patients with an initial clinical diagnosis of renal colic in the emergency department. MATERIAL AND METHODS Multicenter, triple-blind randomized placebo-controlled trial. We randomized 147 patients with a clinical diagnosis of renal colic to an experimental group to receive the N2O/O2 50/50 mixture (n = 70) or a control group to receive 50% oxygen in air (n = 77). Both groups also received conventional analgesia with dexketoprofen plus metamizol and opiates administered sequentially until pain was brought under control; rescue doses of opioids were also available. The endpoints were the reduction in pain intensity expressed on a visual analog scale (VAS) 5 minutes after the start of treatment and the patient's level of satisfaction with treatment on discharge. RESULTS The mean (SD) reduction in pain 5 minutes after starting analgesic treatment was 1.84 (2.05) VAS points in the intervention group and 1.67 (1.91) in the placebo group. The difference was not significant (P = .603). Neither were between-group differences significant at other pain evaluation times (10, 15, 30, and 60 minutes). Treatment was considered satisfactory (􀀀 9 on a VAS) by 53 of the 70 intervention-group patients (75.7%) and 56 of the 77 (72.7%) placebo-group patients (P = .412). Adverse effects appeared in 33 of the 70 patients (48.5%) who received the N2O/O2 mixture and in 19 patients (24.7%) who received the placebo (P = .003). CONCLUSION The addition of N2O/O2 50/50 to standard analgesic therapy does not enhance the efficacy of pain control or the satisfaction of patients treated for renal colic in the emergency department.", "gemini": "MỤC TIÊU Đánh giá hiệu quả của hỗn hợp nitơ oxit và oxy (N2O/O2 50/50) trong việc giảm đau và tăng sự hài lòng ở những bệnh nhân được chẩn đoán lâm sàng ban đầu là đau quặn thận tại khoa cấp cứu. VẬT LIỆU VÀ PHƯƠNG PHÁP Thử nghiệm đối chứng ngẫu nhiên, mù ba, đa trung tâm có sử dụng giả dược. Chúng tôi đã tiến hành phân ngẫu nhiên 147 bệnh nhân được chẩn đoán lâm sàng là đau quặn thận vào một nhóm thực nghiệm để nhận hỗn hợp N2O/O2 50/50 (n = 70) hoặc một nhóm đối chứng để nhận 50% oxy trong không khí (n = 77). Cả hai nhóm cũng được dùng thuốc giảm đau thông thường với dexketoprofen cộng với metamizol và thuốc phiện được dùng tuần tự cho đến khi kiểm soát được cơn đau; liều thuốc phiện giải cứu cũng có sẵn. Các điểm cuối là mức giảm cường độ đau được thể hiện trên thang đo tương tự thị giác (VAS) 5 phút sau khi bắt đầu điều trị và mức độ hài lòng của bệnh nhân đối với việc điều trị khi xuất viện. KẾT QUẢ Mức giảm đau trung bình (SD) sau 5 phút bắt đầu điều trị giảm đau là 1,84 (2,05) điểm VAS ở nhóm can thiệp và 1,67 (1,91) ở nhóm giả dược. Sự khác biệt không đáng kể (P = .603). Sự khác biệt giữa các nhóm cũng không đáng kể ở các thời điểm đánh giá cơn đau khác (10, 15, 30 và 60 phút). Việc điều trị được coi là đạt yêu cầu (􀀀 9 trên thang VAS) đối với 53 trong số 70 bệnh nhân của nhóm can thiệp (75,7%) và 56 trong số 77 (72,7%) bệnh nhân của nhóm giả dược (P = .412). Tác dụng phụ xuất hiện ở 33 trong số 70 bệnh nhân (48,5%) nhận hỗn hợp N2O/O2 và ở 19 bệnh nhân (24,7%) nhận giả dược (P = .003). KẾT LUẬN Việc bổ sung N2O/O2 50/50 vào liệu pháp giảm đau chuẩn không làm tăng hiệu quả kiểm soát cơn đau hoặc sự hài lòng của những bệnh nhân được điều trị đau quặn thận tại khoa cấp cứu."} +{"idx": 1930009, "en": "OBJECTIVES To estimate the prevalence of undiagnosed human immunodeficiency virus (HIV) infection detected by routine testing of patients seeking care in an emergency department and to describe the characteristics associated with new HIV-infection diagnosis. MATERIAL AND METHODS Walk-in patients between the ages of 15 and 75 years who required a blood test were included. Routine fourth-generation enzyme-linked immunoassays were performed to detect HIV infection in all samples extracted. Patients with positive results were referred to the infectious diseases department for monitoring and treatment. RESULTS Blood samples for 1722 patients were analyzed. Twenty-one patients (1.2%) refused to allow their samples to be tested; 19 more samples (1.1%) could not be tested. The prevalence of undiagnosed HIV infection among the remaining 1682 remaining patients was 0.6% (95% CI, 0.23%-0.96%). The prevalence tended to be nonsignificantly higher among patients born outside Spain (0.97% [95% CI, 0.3%-2.20%]) and in 36-50-year-olds (1.46% [95% CI, 0.4%-2.5%]). Characteristics associated with undiagnosed HIV infection were male sex (odds ratio [OR], 5.78 [95% CI, 1.0-31.4]), presenting with a chief complaint that suggested infection (OR, 8.14 [95% CI, 1.6-41.4]), and a history of hepatitis (OR, 5.53 [95% CI, 1.1-27.7]). CONCLUSION The prevalence of undiagnosed HIV infection in our emergency department was high at 0.6%. The rate of patient acceptance of routine HIV testing was high. Strategies that target improving the detection of undiagnosed HIV infection are advisable.", "gemini": "MỤC TIÊU Ước tính tỷ lệ nhiễm virus gây suy giảm miễn dịch ở người (HIV) không được chẩn đoán phát hiện bằng xét nghiệm thường quy ở những bệnh nhân đến khám tại khoa cấp cứu và mô tả các đặc điểm liên quan đến chẩn đoán nhiễm HIV mới. PHƯƠNG PHÁP VÀ VẬT LIỆU Những bệnh nhân đến khám từ 15 đến 75 tuổi cần xét nghiệm máu được đưa vào nghiên cứu. Xét nghiệm miễn dịch liên kết với enzyme thế hệ thứ tư được thực hiện thường quy để phát hiện nhiễm HIV trong tất cả các mẫu được chiết xuất. Những bệnh nhân có kết quả dương tính được chuyển đến khoa bệnh truyền nhiễm để theo dõi và điều trị. KẾT QUẢ Các mẫu máu của 1722 bệnh nhân đã được phân tích. Hai mươi mốt bệnh nhân (1,2%) từ chối xét nghiệm mẫu của họ; 19 mẫu khác (1,1%) không thể xét nghiệm. Tỷ lệ nhiễm HIV không được chẩn đoán trong số 1682 bệnh nhân còn lại là 0,6% (KTCB 95%, 0,23% - 0,96%). Tỷ lệ này có xu hướng cao hơn không đáng kể ở những bệnh nhân sinh ra bên ngoài Tây Ban Nha (0,97% [KTCB 95%, 0,3% - 2,20%]) và ở những người từ 36 đến 50 tuổi (1,46% [KTCB 95%, 0,4% - 2,5%]). Các đặc điểm liên quan đến nhiễm HIV không được chẩn đoán là nam giới (tỷ lệ chênh lệch [OR], 5,78 [KTCB 95%, 1,0 - 31,4]), đến khám với triệu chứng chính gợi ý nhiễm trùng (OR, 8,14 [KTCB 95%, 1,6 - 41,4]), và có tiền sử viêm gan (OR, 5,53 [KTCB 95%, 1,1 - 27,7]). KẾT LUẬN Tỷ lệ nhiễm HIV không được chẩn đoán tại khoa cấp cứu của chúng tôi là cao ở mức 0,6%. Tỷ lệ bệnh nhân chấp nhận xét nghiệm HIV thường quy là cao. Các chiến lược nhằm cải thiện việc phát hiện nhiễm HIV không được chẩn đoán là nên làm."} +{"idx": 1930010, "en": "OBJECTIVES To explore circadian variation in the effectiveness of percutaneous transluminal coronary angioplasty (PTCA) to treat ST-elevation myocardial infarction (STEMI) To explore the effects of circardian variation on infarct extension and in-hospital complications. MATERIAL AND METHODS Observational retrospective cohort study including patients with PTCA-treated STEMI in a tertiary care university hospital between March 2003 and August 2009. The independent variable of interest was the time of onset of STEMI symptoms, grouped in 6-hour time frames. The main outcome variable was PTCA effectiveness. Secondary outcome variables were infarct extension and the presence of in-hospital complications. RESULTS A total of 522 patients records were studied. The mean (SD) age was 62.3 (13.6) years and 404 (77.4%) were men. The largest proportion of PTCA-treated STEMI cases first experienced symptoms between 6 AM and 12 PM (201 cases, 38.5%) (P<.001). PTCA was ineffective in 122 (23.4%). The 6 AM to 12 PM time frame was an independent predictor of PTCA ineffectiveness (odds ratio, 1.79; 95% CI, 1.09-2.94; P=.012). Onset in this interval was also associated with infarct extension but not with in-hospital complications. CONCLUSION A time of onset of STEMI between 6 AM and 12 PM predicts the ineffectiveness of PTCA and greater infarct extension but not in-hospital complications.", "gemini": "MỤC TIÊU Khám phá sự thay đổi theo nhịp sinh học trong hiệu quả của nong mạch vành qua da (PTCA) để điều trị nhồi máu cơ tim có ST chênh lên (STEMI) Khám phá tác động của sự thay đổi theo nhịp sinh học đối với tình trạng nhồi máu lan rộng và các biến chứng trong bệnh viện. PHƯƠNG PHÁP VÀ VẬT LIỆU Nghiên cứu theo dõi hồi cứu quan sát bao gồm những bệnh nhân được điều trị STEMI bằng PTCA tại một bệnh viện đại học chăm sóc sức khỏe cấp ba từ tháng 3 năm 2003 đến tháng 8 năm 2009. Biến số độc lập được quan tâm là thời điểm khởi phát các triệu chứng STEMI, được nhóm theo khung thời gian 6 giờ. Biến số kết quả chính là hiệu quả của PTCA. Các biến số kết quả phụ là tình trạng nhồi máu lan rộng và sự hiện diện của các biến chứng trong bệnh viện. KẾT QUẢ Tổng cộng có 522 hồ sơ bệnh nhân được nghiên cứu. Độ tuổi trung bình (SD) là 62,3 (13,6) và 404 (77,4%) là nam giới. Tỷ lệ lớn nhất các trường hợp STEMI được điều trị bằng PTCA lần đầu tiên xuất hiện các triệu chứng trong khoảng từ 6 giờ sáng đến 12 giờ trưa (201 trường hợp, 38,5%) (P<.001). PTCA không hiệu quả ở 122 (23,4%). Khung thời gian từ 6 giờ sáng đến 12 giờ trưa là một yếu tố dự báo độc lập về tình trạng PTCA không hiệu quả (tỷ lệ chênh lệch, 1,79; KTCB 95%, 1,09 - 2,94; P = 0,012). Khởi phát trong khoảng thời gian này cũng liên quan đến tình trạng nhồi máu lan rộng nhưng không liên quan đến các biến chứng trong bệnh viện. KẾT LUẬN Thời điểm khởi phát STEMI trong khoảng từ 6 giờ sáng đến 12 giờ trưa dự báo tình trạng PTCA không hiệu quả và nhồi máu lan rộng hơn nhưng không phải là các biến chứng trong bệnh viện."} +{"idx": 1930011, "en": "OBJECTIVES Process indicators have been widely used to monitor the way trauma care is provided. We aimed to analyze whether data from a hospital's severe trauma register could facilitate the evaluation of aspects of the initial management of severe injuries. MATERIAL AND METHODS Observational, retrospective population-based study. A working group of experts selected a set of trauma care process indicators relevant to some aspects of initial care, diagnosis, and treatment of severely injured patients. Four of the indicators referred to prehospital care and 5 to hospital care. We calculated the observed and expected compliance rates for all the indicators. RESULTS A total of 1526 cases (44.4%) were analyzed for 2013; 1908 (55.6%) were analyzed for 2014. We were able to evaluate 3 of the 4 prehospital process indicators: endotracheal intubation in patients with a score of 􀀀 8 on the Glasgow coma scale (GCS) (84% compliance), venous access established before hospital arrival (83.4%), and placement of a neck collar to immobilize the cervical spine (72.7%). Compliance for the hospital-phase indicators were as follows: performance of a computed tomography scan of the head within 60 minutes in cases with a GCS of 􀀀 13 (5.3% compliance, craniotomy in candidate patients within 2 hours of diagnosis (65%), diagnostic examination for abdominal injuries within 60 minutes in patients with systolic blood pressure 􀀀 90 mm Hg (89.3%), and therapeutic laparotomy or angiography within 4 hours of abdominal injury in candidate patients with systolic blood pressure 􀀀 90 mm Hg (51.7%). Compliance was 69.9% for the last process indicator: surgical treatment of open fractures within 8 hours of an accident. CONCLUSION Our findings show that a hospital trauma register provides data about care process indicators that can allow us to monitor the quality of care of severely injured patients.", "gemini": "MỤC TIÊU Các chỉ số quy trình đã được sử dụng rộng rãi để theo dõi cách cung cấp dịch vụ chăm sóc chấn thương. Chúng tôi muốn phân tích liệu dữ liệu từ sổ đăng ký chấn thương nghiêm trọng của bệnh viện có thể tạo điều kiện thuận lợi cho việc đánh giá các khía cạnh quản lý ban đầu của các chấn thương nghiêm trọng hay không. PHƯƠNG PHÁP VÀ VẬT LIỆU Nghiên cứu dựa trên dân số, hồi cứu, quan sát. Một nhóm chuyên gia đã chọn một bộ các chỉ số quy trình chăm sóc chấn thương có liên quan đến một số khía cạnh của chăm sóc ban đầu, chẩn đoán và điều trị cho những bệnh nhân bị thương nặng. Bốn trong số các chỉ số đề cập đến chăm sóc trước khi nhập viện và 5 chỉ số đề cập đến chăm sóc tại bệnh viện. Chúng tôi đã tính toán tỷ lệ tuân thủ quan sát và mong đợi cho tất cả các chỉ số. KẾT QUẢ Tổng cộng 1526 trường hợp (44,4%) đã được phân tích cho năm 2013; 1908 (55,6%) đã được phân tích cho năm 2014. Chúng tôi có thể đánh giá 3 trong số 4 chỉ số quy trình trước khi nhập viện: đặt nội khí quản ở những bệnh nhân có điểm 􀀀 8 trên thang điểm hôn mê Glasgow (GCS) (tỷ lệ tuân thủ 84%), đường tĩnh mạch được thiết lập trước khi đến bệnh viện (83,4%), và đặt vòng cổ để cố định cột sống cổ (72,7%). Tỷ lệ tuân thủ đối với các chỉ số giai đoạn tại bệnh viện như sau: thực hiện chụp cắt lớp vi tính đầu trong vòng 60 phút đối với các trường hợp có GCS 􀀀 13 (tỷ lệ tuân thủ 5,3%, phẫu thuật sọ não ở những bệnh nhân đủ điều kiện trong vòng 2 giờ sau khi chẩn đoán (65%), khám chẩn đoán thương tích bụng trong vòng 60 phút ở những bệnh nhân có huyết áp tâm thu 􀀀 90 mm Hg (89,3%), và phẫu thuật nội soi hoặc chụp mạch trong vòng 4 giờ sau khi chấn thương bụng ở những bệnh nhân đủ điều kiện có huyết áp tâm thu 􀀀 90 mm Hg (51,7%). Tỷ lệ tuân thủ là 69,9% đối với chỉ số quy trình cuối cùng: điều trị phẫu thuật gãy xương hở trong vòng 8 giờ sau khi xảy ra tai nạn. KẾT LUẬN Những phát hiện của chúng tôi cho thấy sổ đăng ký chấn thương của bệnh viện cung cấp dữ liệu về các chỉ số quy trình chăm sóc có thể cho phép chúng tôi theo dõi chất lượng chăm sóc cho những bệnh nhân bị thương nặng."} +{"idx": 1930012, "en": "OBJECTIVES To explore the frequency of lack of autonomous mobility and factors related to it in patients requiring prehospital emergency services. MATERIAL AND METHODS Cross-sectional, observational, retrospective analysis. We identified a stratified random sample of patients attended by the public health emergency service of Andalusia in 2011. RESULTS The sample included 280 patients with a mean age of 56 years; 63.9% were men. The majority were attended in homes and streets or other public spaces. Most were transported to a hospital in a mobile intensive care unit. The reasons for calling the service were most often related to chest pain or injuries. Loss of autonomous mobility was seen more often in men, when care was required in a public area, when there were injuries or altered vital signs, and when the patient required transport in a mobile intensive care unit. Dependence on others was significantly associated with the presence of a prior condition before the patient required transport (odds ratio [OR], 3.27; 95% CI, 1.60-6.33), the use of immobilization strategies (OR, 7.71; 95% CI, 1.7-34.96], and the use of ambulance transport (OR, 4.35; 95% CI, 1.63-11.60]. The following 2 variables were at the limit of significance: pain during the care process (OR, 1.13; 95% CI, 0.99-1.29) and age (OR, 0.46; 95% CI, 0.97-1.01). CONCLUSION The profile we identified for patients and situations in which patients cannot move autonomously during prehospital emergency care can be used to plan preventive strategies to ensure patient safety.", "gemini": "MỤC TIÊU Khảo sát tần suất thiếu khả năng vận động tự chủ và các yếu tố liên quan ở những bệnh nhân cần dịch vụ cấp cứu tiền viện. PHƯƠNG PHÁP VÀ VẬT LIỆU Phân tích hồi cứu, quan sát, cắt ngang. Chúng tôi xác định một mẫu ngẫu nhiên phân tầng những bệnh nhân được dịch vụ cấp cứu y tế công cộng của Andalusia điều trị vào năm 2011. KẾT QUẢ Mẫu nghiên cứu gồm 280 bệnh nhân với độ tuổi trung bình là 56; 63,9% là nam giới. Phần lớn được điều trị tại nhà và đường phố hoặc những không gian công cộng khác. Hầu hết được chuyển đến bệnh viện bằng xe cấp cứu lưu động. Các lý do gọi dịch vụ thường liên quan đến đau ngực hoặc chấn thương. Mất khả năng vận động tự chủ thường gặp hơn ở nam giới, khi cần chăm sóc ở nơi công cộng, khi có chấn thương hoặc các dấu hiệu sinh tồn bị thay đổi, và khi bệnh nhân cần vận chuyển bằng xe cấp cứu lưu động. Sự phụ thuộc vào người khác có liên quan đáng kể đến tình trạng bệnh lý trước đó trước khi bệnh nhân cần vận chuyển (tỷ lệ nguy cơ [OR], 3,27; KTCB 95%, 1,60-6,33), sử dụng các chiến lược cố định bất động (OR, 7,71; KTCB 95%, 1,7-34,96] và sử dụng vận chuyển bằng xe cứu thương (OR, 4,35; KTCB 95%, 1,63-11,60]. 2 biến sau đây nằm ở ngưỡng có ý nghĩa: đau trong quá trình chăm sóc (OR, 1,13; KTCB 95%, 0,99-1,29) và tuổi (OR, 0,46; KTCB 95%, 0,97-1,01). KẾT LUẬN Hồ sơ bệnh nhân và các tình huống mà bệnh nhân không thể tự vận động trong quá trình cấp cứu tiền viện mà chúng tôi xác định có thể được sử dụng để lập kế hoạch các chiến lược phòng ngừa nhằm đảm bảo an toàn cho bệnh nhân."} +{"idx": 1930013, "en": "OBJECTIVES To evaluate the correlation between variations in ultrasound-measured diaphragm movement and changes in the arterial partial pressure of carbon dioxide (PCO2) after the start of noninvasive ventilation (NIV). MATERIAL AND METHODS RDescriptive study of a prospective case series comprised of nonconsecutive patients aged 18 years or older with hypercapnic respiratory failure who were placed on NIV in an emergency department. We recorded clinical data, blood gas measurements, and ultrasound measurements of diaphragm movement. RESULTS Twenty-one patients with a mean (SD) age of 83 (13) years were studied; 11 (52.4%) were women. The mean (SD) range of diaphragm movement and PCO2 values at 4 moments were as follows: 1) at baseline: diaphragm movement, 13.90 (7.7) mm and PCO2, 71.75 (11.4) mm Hg; 2) after 15 minutes on NIV: diaphragm movement, 17.10 (9.1) mm; 3) at 1 hour: diaphragm movement, 22.40 (10.4) mm and PCO2, 63.45 (16.0) mm Hg; and 4) at 3 hours: diaphragm movement, 26.60 (19.5) mm and PCO2, 61.85 (13.0) mm Hg. We detected a statistically significant correlation between the difference in range of diaphragm movement at baseline and at 15 minutes and the decrease in PCO2 after 1 hour of NIV (r=-0.489, P=.035). CONCLUSION In patients with hypercapnic respiratory failure, the increase in range of diaphragm movement 15 minutes after starting NIV is associated with a decrease in PCO2 after 1 hour.", "gemini": "MỤC TIÊU Đánh giá mối tương quan giữa các biến động về cử động cơ hoành đo bằng siêu âm và những thay đổi về áp suất riêng phần động mạch của carbon dioxide (PCO2) sau khi bắt đầu thở máy không xâm lấn (NIV). PHƯƠNG PHÁP VÀ VẬT LIỆU Nghiên cứu mô tả về một loạt ca bệnh có triển vọng bao gồm những bệnh nhân không liên tiếp từ 18 tuổi trở lên bị suy hô hấp tăng CO2 được đặt máy NIV tại khoa cấp cứu. Chúng tôi ghi lại các dữ liệu lâm sàng, các phép đo khí máu và các phép đo siêu âm về cử động cơ hoành. KẾT QUẢ Hai mươi mốt bệnh nhân với độ tuổi trung bình (SD) là 83 (13) đã được nghiên cứu; 11 (52,4%) là nữ. Phạm vi trung bình (SD) của cử động cơ hoành và các giá trị PCO2 tại 4 thời điểm như sau: 1) lúc ban đầu: cử động cơ hoành, 13,90 (7,7) mm và PCO2, 71,75 (11,4) mm Hg; 2) sau 15 phút thở máy NIV: cử động cơ hoành, 17,10 (9,1) mm; 3) sau 1 giờ: cử động cơ hoành, 22,40 (10,4) mm và PCO2, 63,45 (16,0) mm Hg; và 4) sau 3 giờ: cử động cơ hoành, 26,60 (19,5) mm và PCO2, 61,85 (13,0) mm Hg. Chúng tôi phát hiện ra mối tương quan có ý nghĩa thống kê giữa sự khác biệt về phạm vi cử động cơ hoành lúc ban đầu và sau 15 phút và sự giảm PCO2 sau 1 giờ NIV (r=-0,489, P=.035). KẾT LUẬN Ở những bệnh nhân bị suy hô hấp tăng CO2, sự gia tăng phạm vi cử động cơ hoành 15 phút sau khi bắt đầu NIV có liên quan đến sự giảm PCO2 sau 1 giờ."} +{"idx": 1930014, "en": "We analyzed epidemiologic and clinical characteristics, treatment received, and clinical course in cases of pepper spray injury treated in the chemical decontamination area of an emergency department over a 5-year period. A total of 15 patients with a mean (SD) age of 25 (8.3) years were included. Thirteen were men and 53% were foreign-born. All uses of pepper spray occurred during muggings that to place mainly in the early hours of the morning (66.7% between 4:30 and 6:30 AM). Two or more attackers were injured in 60% of the events. Seventy-three percent of the attacks took place on the street and the median delay in reaching the emergency department was 30 minutes. The face and eyes were usually injured, and symptoms included itching, irritation, burning, and pain. On arrival, the patients were treated immediately (mean delay, 3 minutes) with hypertonic saline, an amphoteric surfactant, and a chelating agent in 80% of the cases, in which improvement was substantial as mean pain scale scores changed from 7.2 on admission to 3 on discharge (P=.009). Patients spent an average of 40 minutes in the treatment area. Eleven patients required further care from an ophthalmologist and topical medication for symptoms. Outcomes were good and there were no complications in any of the cases. We conclude that pepper sprays are being used as an aid to self defense in muggings in our community. Decontamination measures have priority as they alleviate symptoms and prevent long-lasting effects.", "gemini": "Chúng tôi đã phân tích các đặc điểm dịch tễ học và lâm sàng, phương pháp điều trị được áp dụng và diễn biến lâm sàng trong các trường hợp bị thương do bình xịt hơi cay được điều trị tại khu vực khử nhiễm hóa chất của khoa cấp cứu trong khoảng thời gian 5 năm. Tổng cộng có 15 bệnh nhân với độ tuổi trung bình (SD) là 25 (8,3) đã được đưa vào nghiên cứu. Mười ba người là nam giới và 53% là người nước ngoài. Tất cả các trường hợp sử dụng bình xịt hơi cay đều xảy ra trong các vụ cướp giật chủ yếu vào sáng sớm (66,7% trong khoảng thời gian từ 4:30 đến 6:30 sáng). Hai hoặc nhiều kẻ tấn công bị thương trong 60% các sự kiện. Bảy mươi ba phần trăm các vụ tấn công diễn ra trên đường phố và thời gian trung bình để đến khoa cấp cứu là 30 phút. Mặt và mắt thường bị thương, và các triệu chứng bao gồm ngứa, kích ứng, bỏng rát và đau. Khi đến nơi, bệnh nhân được điều trị ngay lập tức (thời gian trung bình là 3 phút) bằng dung dịch muối ưu trương, chất hoạt động bề mặt lưỡng tính và chất tạo phức trong 80% các trường hợp, trong đó cải thiện đáng kể vì điểm thang đau trung bình thay đổi từ 7,2 khi nhập viện xuống còn 3 khi xuất viện (P=.009). Bệnh nhân dành trung bình 40 phút tại khu vực điều trị. Mười một bệnh nhân cần được bác sĩ nhãn khoa chăm sóc thêm và dùng thuốc tại chỗ để điều trị các triệu chứng. Kết quả điều trị tốt và không có biến chứng nào trong bất kỳ trường hợp nào. Chúng tôi kết luận rằng bình xịt hơi cay đang được sử dụng như một công cụ tự vệ trong các vụ cướp giật trong cộng đồng của chúng tôi. Các biện pháp khử nhiễm được ưu tiên vì chúng làm giảm các triệu chứng và ngăn ngừa các tác động lâu dài."} +{"idx": 1930015, "en": "[This corrects the article on p. 118 in vol. 4.].", "gemini": "[Điều này sửa lỗi bài viết ở trang 118 trong tập 4.]."} +{"idx": 1930016, "en": "[This corrects the article on p. 155 in vol. 7.].", "gemini": "[Điều này sửa lỗi bài viết ở trang 155 trong tập 7.]."} +{"idx": 1930017, "en": "A body of literature connects parental emotion socialization behaviors to child outcomes, though little research attention has been devoted to parents' culturally embedded socialization goals that influence their socialization behaviors in diverse samples. In the present study, we examined interrelations among maternal socialization goals, emotion socialization behaviors, and child functioning in families from 2 major Asian countries, China and India. A total of 305 6th and 7th grade children and their mothers across both countries participated. Mothers completed measures of their socialization goals, their responses to children's emotions, child behavior problems, and children completed a measure of emotion regulation (ER) at a single point in time. Factor analysis of the measure of parental responses to children's emotions yielded 2 factors (supportive and nonsupportive responses), with some items from the commonly used parent-report measure Coping with Children's Negative Emotions Scale (Fabes, Eisenberg, & Bernzweig, 1990), while others reflected culturally salient socialization approaches in Asia (i.e., child training, explanation). Using these data-driven supportive and nonsupportive response composites, mediation models were tested for the full sample. Reports of mothers' supportive responses and child ER sequentially mediated the relation between maternal relational socialization goals and child internalizing problems. Reports of child emotion dysregulation mediated the relation between maternal nonsupportive responses and child externalizing problems. Findings of this study highlight the relevance of culturally salient parental socialization goals and socialization behaviors in understanding child functioning in diverse cultural groups. (PsycINFO Database Record", "gemini": "Một loạt các tài liệu kết nối hành vi xã hội hóa cảm xúc của cha mẹ với kết quả của trẻ em, mặc dù ít sự chú ý của nghiên cứu được dành cho các mục tiêu xã hội hóa nhúng văn hóa của cha mẹ ảnh hưởng đến hành vi xã hội hóa của họ trong các mẫu khác nhau. Trong nghiên cứu hiện tại, chúng tôi đã kiểm tra mối quan hệ giữa các mục tiêu xã hội hóa của bà mẹ, hành vi xã hội hóa cảm xúc và chức năng của trẻ trong các gia đình từ 2 quốc gia châu Á lớn là Trung Quốc và Ấn Độ. Tổng cộng có 305 trẻ em lớp 6 và lớp 7 và mẹ của chúng ở cả hai quốc gia đã tham gia. Các bà mẹ đã hoàn thành các biện pháp về mục tiêu xã hội hóa của họ, phản ứng của họ đối với cảm xúc của trẻ em, các vấn đề về hành vi của trẻ em và trẻ em đã hoàn thành một biện pháp điều chỉnh cảm xúc (ER) tại một thời điểm duy nhất. Phân tích nhân tố của biện pháp về phản ứng của cha mẹ đối với cảm xúc của trẻ em đã đưa ra 2 yếu tố (phản ứng hỗ trợ và không hỗ trợ), với một số mục từ biện pháp báo cáo của cha mẹ thường được sử dụng là Thang đo đối phó với cảm xúc tiêu cực của trẻ em (Fabes, Eisenberg, & Bernzweig, 1990), trong khi những mục khác phản ánh các phương pháp xã hội hóa nổi bật về mặt văn hóa ở Châu Á (tức là đào tạo trẻ em, giải thích). Sử dụng các hợp chất phản hồi hỗ trợ và không hỗ trợ theo dữ liệu này, các mô hình trung gian đã được kiểm tra cho toàn bộ mẫu. Báo cáo về phản ứng hỗ trợ của bà mẹ và ER của trẻ em lần lượt trung gian mối quan hệ giữa các mục tiêu xã hội hóa quan hệ của bà mẹ và các vấn đề nội tâm của trẻ em. Báo cáo về chứng rối loạn cảm xúc ở trẻ em là trung gian cho mối quan hệ giữa các phản ứng không hỗ trợ của bà mẹ và các vấn đề đối ngoại của trẻ em. Những phát hiện của nghiên cứu này làm nổi bật sự phù hợp của các mục tiêu xã hội hóa của cha mẹ và hành vi xã hội hóa nổi bật về mặt văn hóa trong việc hiểu chức năng của trẻ em trong các nhóm văn hóa đa dạng. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930018, "en": "Many contemporary accounts of human reasoning assume that the mind is equipped with multiple heuristics that could be deployed to perform a given task. This raises the question of how the mind determines when to use which heuristic. To answer this question, we developed a rational model of strategy selection, based on the theory of rational metareasoning developed in the artificial intelligence literature. According to our model people learn to efficiently choose the strategy with the best cost-benefit tradeoff by learning a predictive model of each strategy's performance. We found that our model can provide a unifying explanation for classic findings from domains ranging from decision-making to arithmetic by capturing the variability of people's strategy choices, their dependence on task and context, and their development over time. Systematic model comparisons supported our theory, and 4 new experiments confirmed its distinctive predictions. Our findings suggest that people gradually learn to make increasingly more rational use of fallible heuristics. This perspective reconciles the 2 poles of the debate about human rationality by integrating heuristics and biases with learning and rationality. (PsycINFO Database Record", "gemini": "Nhiều mô tả đương đại về lý luận của con người cho rằng tâm trí được trang bị nhiều phương pháp tìm kiếm có thể được triển khai để thực hiện một nhiệm vụ nhất định. Điều này đặt ra câu hỏi làm thế nào để tâm trí xác định khi nào sử dụng phương pháp tìm kiếm nào. Để trả lời câu hỏi này, chúng tôi đã phát triển một mô hình hợp lý về lựa chọn chiến lược, dựa trên lý thuyết về siêu lý luận hợp lý được phát triển trong tài liệu về trí tuệ nhân tạo. Theo mô hình của chúng tôi, mọi người học cách lựa chọn hiệu quả chiến lược có sự đánh đổi chi phí-lợi ích tốt nhất bằng cách học một mô hình dự đoán về hiệu suất của từng chiến lược. Chúng tôi thấy rằng mô hình của chúng tôi có thể cung cấp một lời giải thích thống nhất cho những phát hiện cổ điển từ các lĩnh vực, từ ra quyết định đến số học bằng cách nắm bắt tính biến đổi trong các lựa chọn chiến lược của mọi người, sự phụ thuộc của họ vào nhiệm vụ và bối cảnh, và sự phát triển của họ theo thời gian. Các so sánh mô hình có hệ thống đã hỗ trợ lý thuyết của chúng tôi và 4 thử nghiệm mới đã xác nhận các dự đoán đặc biệt của nó. Những phát hiện của chúng tôi cho thấy rằng mọi người dần dần học cách sử dụng các phương pháp tìm kiếm dễ sai lầm một cách ngày càng hợp lý hơn. Quan điểm này hòa giải 2 cực của cuộc tranh luận về tính hợp lý của con người bằng cách tích hợp các phương pháp tìm kiếm và thành kiến với việc học và tính hợp lý. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930019, "en": "We present a dynamic model of memory that integrates the processes of perception, retrieval from knowledge, retrieval of events, and decision making as these evolve from 1 moment to the next. The core of the model is that recognition depends on tracking changes in familiarity over time from an initial baseline generally determined by context, with these changes depending on the availability of different kinds of information at different times. A mathematical implementation of this model leads to precise, accurate predictions of accuracy, response time, and speed-accuracy trade-off in episodic recognition at the levels of both groups and individuals across a variety of paradigms. Our approach leads to novel insights regarding word frequency, speeded responding, context reinstatement, short-term priming, similarity, source memory, and associative recognition, revealing how the same set of core dynamic principles can help unify otherwise disparate phenomena in the study of memory. (PsycINFO Database Record", "gemini": "Chúng tôi trình bày một mô hình bộ nhớ động tích hợp các quá trình nhận thức, truy xuất từ kiến thức, truy xuất sự kiện và ra quyết định khi chúng phát triển từ thời điểm này sang thời điểm khác. Cốt lõi của mô hình là sự nhận biết phụ thuộc vào việc theo dõi các thay đổi về sự quen thuộc theo thời gian từ một mức cơ sở ban đầu thường được xác định bởi bối cảnh, với những thay đổi này phụ thuộc vào tính khả dụng của các loại thông tin khác nhau tại các thời điểm khác nhau. Việc triển khai toán học mô hình này dẫn đến các dự đoán chính xác, chính xác về độ chính xác, thời gian phản hồi và sự đánh đổi tốc độ-độ chính xác trong nhận dạng từng phần ở cấp độ của cả nhóm và cá nhân trên nhiều mô hình khác nhau. Phương pháp tiếp cận của chúng tôi dẫn đến những hiểu biết mới về tần suất từ, phản ứng tăng tốc, khôi phục bối cảnh, mồi ngắn hạn, tính tương tự, bộ nhớ nguồn và nhận dạng liên kết, tiết lộ cách cùng một tập hợp các nguyên tắc động cốt lõi có thể giúp thống nhất các hiện tượng khác nhau trong nghiên cứu về trí nhớ. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930020, "en": "The young field of research on dark personality traits (i.e., socially aversive traits such as psychopathy, narcissism, and Machiavellianism) is gaining momentum. This Special Section examines the nature, origins, development, and sequelae of dark traits, underscoring their largely unappreciated relevance to abnormal psychology. The articles in this section adopt diverse perspectives (e.g., clinical, developmental, organizational, social personality), use diverse methods (e.g., longitudinal, experience sampling), and sample diverse populations (e.g., offenders, psychiatric outpatients, schoolchildren). As an introduction to the Special Section, this article identifies key conceptual and methodological challenges to the field of dark personality research. Its aim is to spur novel approaches to how dark personality traits are implicated in psychopathology and abnormal behavior more broadly. (PsycINFO Database Record", "gemini": "Lĩnh vực nghiên cứu còn non trẻ về các đặc điểm tính cách đen tối (tức là các đặc điểm xa lánh xã hội như tâm thần phân liệt, tự ái và chủ nghĩa Machiavelli) đang phát triển mạnh. Phần đặc biệt này xem xét bản chất, nguồn gốc, sự phát triển và hậu quả của các đặc điểm đen tối, nhấn mạnh sự liên quan phần lớn chưa được đánh giá cao của chúng đối với tâm lý học bất thường. Các bài viết trong phần này áp dụng các quan điểm đa dạng (ví dụ: lâm sàng, phát triển, tổ chức, tính cách xã hội), sử dụng các phương pháp đa dạng (ví dụ: theo chiều dọc, lấy mẫu kinh nghiệm) và lấy mẫu các quần thể đa dạng (ví dụ: người phạm tội, bệnh nhân ngoại trú tâm thần, trẻ em trong độ tuổi đi học). Là phần giới thiệu cho Phần đặc biệt, bài viết này xác định những thách thức chính về mặt khái niệm và phương pháp đối với lĩnh vực nghiên cứu tính cách đen tối. Mục đích của nó là thúc đẩy các cách tiếp cận mới về cách các đặc điểm tính cách đen tối liên quan đến bệnh lý tâm thần và hành vi bất thường nói chung. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930021, "en": "In this paper, we argue that prefrontal cortex ontogenetic functional development is best understood through an ecological lens. We first begin by reviewing evidence supporting the existing consensus that PFC structural and functional development is protracted based on maturational constraints. We then examine recent findings from neuroimaging studies in infants, early life stress research, and connectomics that support the novel hypothesis that PFC functional development is driven by reciprocal processes of neural adaptation and niche construction. We discuss implications and predictions of this model for redefining the construct of executive functions and for informing typical and atypical child development. This ecological account of PFC functional development moves beyond descriptions of development that are characteristic of existing frameworks, and provides novel insights into the mechanisms of developmental change, including its catalysts and influences. (PsycINFO Database Record", "gemini": "Trong bài báo này, chúng tôi lập luận rằng sự phát triển chức năng của vỏ não trước trán có thể được hiểu rõ nhất thông qua lăng kính sinh thái. Đầu tiên, chúng tôi bắt đầu bằng cách xem xét các bằng chứng hỗ trợ sự đồng thuận hiện tại rằng sự phát triển cấu trúc và chức năng của PFC bị kéo dài dựa trên các ràng buộc về sự trưởng thành. Sau đó, chúng tôi xem xét các phát hiện gần đây từ các nghiên cứu hình ảnh thần kinh ở trẻ sơ sinh, nghiên cứu căng thẳng ở giai đoạn đầu đời và kết nối học hỗ trợ cho giả thuyết mới rằng sự phát triển chức năng của PFC được thúc đẩy bởi các quá trình thích ứng thần kinh và xây dựng thích hợp. Chúng tôi thảo luận về những hàm ý và dự đoán của mô hình này để xác định lại cấu trúc của các chức năng điều hành và để thông báo về sự phát triển điển hình và không điển hình của trẻ em. Bản tường trình sinh thái này về sự phát triển chức năng của PFC vượt ra ngoài các mô tả về sự phát triển đặc trưng của các khuôn khổ hiện có và cung cấp những hiểu biết mới về các cơ chế thay đổi phát triển, bao gồm cả chất xúc tác và ảnh hưởng của nó. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930022, "en": "The dark triad of personality has traditionally been defined by 3 interrelated constructs, defined as Narcissism, Machiavellianism, and Psychopathy. Although the content of each of these constructs is clearly represented in childhood maladaptive trait measures, no studies have jointly addressed the prospective developmental course of this core set of maladaptive characteristics throughout childhood and adolescence. The current study uses latent growth modeling to explore how early dark traits develop over time, relying on a selected set of 6 childhood maladaptive traits that conceptually cover the adult dark triad. Across a 5-wave multi-informant design spanning 10 years of childhood, adolescence, and emerging adulthood (Nwave 1 = 717, 54.4% girls, age range T1 = 8-14.7 years, mean age = 10.73), results indicate that childhood dark traits show to some extent shared growth across time, although notable unique growth variance was also observed. Early dark traits further demonstrate significant association patterns with an adult dark triad measure across informants and are increasingly able to discriminate among more and less prototypical profiles of adult dark triad scores. Findings are discussed from a developmental psychopathology framework, underscoring that the proposed set of childhood dark traits represents a meaningful developmental precursor of the adult dark triad. (PsycINFO Database Record", "gemini": "Bộ ba tính cách đen tối theo truyền thống được định nghĩa bởi 3 cấu trúc có liên quan, được định nghĩa là Tự ái, Chủ nghĩa Machiavelli và Tâm thần. Mặc dù nội dung của từng cấu trúc này được thể hiện rõ ràng trong các biện pháp đặc điểm thích ứng kém ở trẻ em, nhưng không có nghiên cứu nào giải quyết chung về quá trình phát triển triển vọng của tập hợp các đặc điểm thích ứng kém cốt lõi này trong suốt thời thơ ấu và vị thành niên. Nghiên cứu hiện tại sử dụng mô hình tăng trưởng tiềm ẩn để khám phá cách các đặc điểm đen tối ở giai đoạn đầu phát triển theo thời gian, dựa vào một tập hợp được lựa chọn gồm 6 đặc điểm thích ứng kém ở trẻ em về mặt khái niệm bao gồm bộ ba đen tối ở người lớn. Trong một thiết kế đa thông tin 5 sóng kéo dài 10 năm thời thơ ấu, vị thành niên và tuổi trưởng thành mới nổi (Nwave 1 = 717, 54,4% là nữ, độ tuổi T1 = 8-14,7 năm, tuổi trung bình = 10,73), kết quả cho thấy các đặc điểm đen tối ở trẻ em cho thấy sự tăng trưởng được chia sẻ ở một mức độ nào đó theo thời gian, mặc dù sự biến đổi tăng trưởng độc đáo đáng chú ý cũng được quan sát thấy. Các đặc điểm đen tối ở giai đoạn đầu tiếp tục chứng minh các mô hình liên kết đáng kể với biện pháp bộ ba đen tối ở người lớn trên nhiều người cung cấp thông tin và ngày càng có khả năng phân biệt giữa các cấu hình nguyên mẫu nhiều hơn và ít hơn về số điểm bộ ba đen tối ở người lớn. Các phát hiện được thảo luận từ một khuôn khổ bệnh lý tâm lý phát triển, nhấn mạnh rằng tập hợp các đặc điểm đen tối ở trẻ em được đề xuất đại diện cho một tiền thân phát triển có ý nghĩa của bộ ba đen tối ở người lớn. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930023, "en": "Research on how dark personality traits develop and relate to risky behaviors and family relations during adolescence is scarce. This study used a person-oriented approach to examine (a) whether distinct groups of adolescents could be identified based on their developmental profiles of callous-unemotional (CU), grandiose manipulative (GM), and dysfunctional impulsivity (DI) traits and (b) whether these groups differ in their problem behaviors and parent-adolescent relationship quality. Latent class growth analyses on 4-wave data of 1,131 Dutch adolescents revealed 3 personality profiles: (1) a dark impulsive group (13.9%), with high scores on all 3 traits (CU, GM, and DI) that were stable over time; (2) an impulsive group (26.1%), with high and increasing levels of impulsivity and relatively low scores on CU and GM; and (3) and a low risk group (60.0%), with relatively low levels on all 3 personality characteristics, with impulsivity decreasing over time. Compared with adolescents in the low risk group, adolescents in the dark impulsive and impulsive groups reported higher initial levels of substance use, sexual risk behaviors, permissive sexual attitudes, parent-adolescent conflict, and lower parent-adolescent satisfaction, as well as greater increases in sexual risk behavior over time. Compared with adolescents in the impulsive group, those in the dark impulsive group showed the highest levels of risk behaviors. Hence, dark personality traits coupled with impulsivity may be indicative of an earlier and more severe trajectory of problem behaviors that may differ from the trajectory of youth who are only impulsive. (PsycINFO Database Record", "gemini": "Nghiên cứu về cách các đặc điểm tính cách đen tối phát triển và liên quan đến hành vi mạo hiểm và quan hệ gia đình trong thời kỳ vị thành niên còn rất khan hiếm. Nghiên cứu này đã sử dụng một phương pháp tiếp cận theo định hướng cá nhân để kiểm tra (a) liệu có thể xác định được các nhóm thanh thiếu niên riêng biệt dựa trên hồ sơ phát triển của họ về các đặc điểm vô cảm-thiếu cảm xúc (CU), thao túng vĩ đại (GM) và bốc đồng rối loạn chức năng (DI) hay không và (b) liệu các nhóm này có khác nhau về hành vi có vấn đề và chất lượng mối quan hệ cha mẹ-con cái hay không. Các phân tích tăng trưởng lớp tiềm ẩn trên dữ liệu 4 sóng của 1.131 thanh thiếu niên Hà Lan đã tiết lộ 3 hồ sơ tính cách: (1) một nhóm bốc đồng đen tối (13,9%), với số điểm cao ở cả 3 đặc điểm (CU, GM và DI) ổn định theo thời gian; (2) một nhóm bốc đồng (26,1%), với mức độ bốc đồng cao và ngày càng tăng và số điểm tương đối thấp về CU và GM; và (3) và một nhóm rủi ro thấp (60,0%), với mức độ tương đối thấp ở cả 3 đặc điểm tính cách, với sự bốc đồng giảm dần theo thời gian. So với thanh thiếu niên trong nhóm rủi ro thấp, thanh thiếu niên trong nhóm bốc đồng đen tối và bốc đồng báo cáo mức độ sử dụng chất gây nghiện ban đầu cao hơn, hành vi rủi ro về tình dục, thái độ tình dục dễ dãi, xung đột cha mẹ-con cái và sự hài lòng của cha mẹ-con cái thấp hơn, cũng như sự gia tăng lớn hơn về hành vi rủi ro về tình dục theo thời gian. So với thanh thiếu niên trong nhóm bốc đồng, những thanh thiếu niên trong nhóm bốc đồng đen tối có mức hành vi rủi ro cao nhất. Do đó, các đặc điểm tính cách đen tối kết hợp với sự bốc đồng có thể chỉ ra một quỹ đạo hành vi có vấn đề sớm hơn và nghiêm trọng hơn, có thể khác với quỹ đạo của những thanh thiếu niên chỉ bốc đồng. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930024, "en": "Psychopathy refers to a heterogeneous set of harmful dark traits and behaviors, including superficial charm, callousness, irresponsibility, and antisocial behavior. The triarchic psychopathy model (TriPM) posits that psychopathy is the combination of 3 traits: boldness, disinhibition, and meanness. However, little research has examined the concurrent and developmental correlates of these traits. We developed TriPM scales from the NEO Personality Inventory-Revised using an empirical-derived approach in a high-risk sample of 561 young adults (ages 17-25; 70.2% male). Concurrent correlates and developmental precursors of each scale were examined longitudinally using cross-informant reports from 3 critical developmental periods (ages 3-5; 9-11; 15-17). Using this approach, we identified consistent developmental precursors and concurrent correlates of boldness, including lower reactive control, fewer internalizing traits, and greater resiliency. Additionally, starting in adolescence we found that disinhibition was related to lower reactive control, more externalizing problems, substance use, and internalizing traits. Finally, although meanness demonstrated some expected concurrent relationships with criterion variables in early adulthood (e.g., lower adaptive functioning), we identified few consistent developmental precursors of meanness. Thus, a NEO-based approach to measuring the TriPM was successful in delineating boldness, disinhibition, and, to a lesser extent, meanness cross-sectionally during early adulthood. However, only boldness showed relative stability from developmental precursors in early childhood to our TriPM scale in early adulthood. (PsycINFO Database Record", "gemini": "Rối loạn nhân cách là một tập hợp các đặc điểm và hành vi có hại, bao gồm sự quyến rũ hời hợt, vô cảm, vô trách nhiệm và hành vi chống đối xã hội. Mô hình rối loạn nhân cách ba chiều (TriPM) đưa ra giả thuyết rằng rối loạn nhân cách là sự kết hợp của 3 đặc điểm: táo bạo, vô ức chế và tàn nhẫn. Tuy nhiên, ít nghiên cứu nào xem xét các mối tương quan đồng thời và phát triển của những đặc điểm này. Chúng tôi đã phát triển thang đo TriPM từ Bản kiểm kê tính cách NEO-Sửa đổi bằng cách sử dụng phương pháp tiếp cận theo kinh nghiệm trong một mẫu có nguy cơ cao gồm 561 thanh niên (tuổi từ 17 đến 25; 70,2% là nam). Các mối tương quan đồng thời và các tiền thân phát triển của mỗi thang đo đã được kiểm tra theo chiều dọc bằng cách sử dụng các báo cáo từ 3 giai đoạn phát triển quan trọng (tuổi 3-5; 9-11; 15-17). Sử dụng phương pháp tiếp cận này, chúng tôi đã xác định được các tiền thân phát triển nhất quán và các mối tương quan đồng thời của sự táo bạo, bao gồm kiểm soát phản ứng thấp hơn, ít đặc điểm nội tâm hóa hơn và khả năng phục hồi lớn hơn. Ngoài ra, bắt đầu từ tuổi vị thành niên, chúng tôi phát hiện ra rằng sự vô ức chế có liên quan đến kiểm soát phản ứng thấp hơn, nhiều vấn đề ngoại hóa hơn, sử dụng chất gây nghiện và các đặc điểm nội tâm hóa. Cuối cùng, mặc dù sự tàn nhẫn cho thấy một số mối quan hệ đồng thời như mong đợi với các biến tiêu chuẩn ở người trưởng thành sớm (ví dụ: chức năng thích nghi thấp hơn), chúng tôi xác định được một số tiền thân phát triển nhất quán của sự tàn nhẫn. Do đó, phương pháp tiếp cận dựa trên NEO để đo lường TriPM đã thành công trong việc phân định sự táo bạo, vô ức chế và ở mức độ thấp hơn là sự tàn nhẫn theo chiều cắt ngang trong thời kỳ đầu trưởng thành. Tuy nhiên, chỉ có sự táo bạo mới cho thấy sự ổn định tương đối từ các tiền thân phát triển trong thời thơ ấu đến thang đo TriPM của chúng tôi ở thời kỳ đầu trưởng thành. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930025, "en": "It is widely known that personality traits collectively discussed as the Dark Triad are antagonistic and associated with poor interpersonal relationships, but few studies have examined how specific facets of antagonism are associated with psychosocial adjustment or how antagonism relates to psychosocial adjustment other than interpersonal functioning. The purpose of this study was to examine how 6 antagonism facets-manipulativeness, grandiosity, attention-seeking, hostility, callousness, and deceitfulness-relate to comprehensive psychosocial functional domains (i.e., well-being, interpersonal relationships, basic daily functioning) using information about both antagonism and functioning from 3 sources-self, informant, and interviewer. Data were from 318 primary participants and informants. We present 3 main findings: (1) When psychosocial functioning and antagonism traits were both rated by informants, all psychosocial disability domains were consistently positively associated with antagonism traits. (2) We next created a single psychosocial-disability factor score via principal factors analysis of all raters' psychosocial-functioning scores. When all 3 raters' reports of domain-level antagonism were used as independent variables in a simultaneous regression analysis to predict this overall functioning score, informant-reported antagonism most strongly predicted psychosocial functioning, followed by interviewer-rated antagonism. (3) We then created 6 facet scores by summing the 3 raters' scores on each. When we used these scores to predict psychosocial functioning, hostility was the main trait significantly predicting psychosocial functioning. The study provides further insight into associations of psychosocial disability with antagonism facets from different raters' perspectives. The findings thus further our understanding of psychosocial outcomes associated with antagonism, the core of the dark traits. (PsycINFO Database Record", "gemini": "Người ta biết rộng rãi rằng các đặc điểm tính cách được thảo luận chung là Bộ ba đen tối có tính đối kháng và liên quan đến các mối quan hệ giữa các cá nhân kém, nhưng ít nghiên cứu nào xem xét cách các khía cạnh cụ thể của tính đối kháng có liên quan đến sự điều chỉnh tâm lý xã hội như thế nào hoặc sự đối kháng liên quan đến sự điều chỉnh tâm lý xã hội như thế nào ngoài chức năng giữa các cá nhân. Mục đích của nghiên cứu này là kiểm tra cách 6 khía cạnh đối kháng - thao túng, tự phụ, tìm kiếm sự chú ý, thù địch, vô cảm và gian dối - liên quan đến các phạm vi chức năng tâm lý xã hội toàn diện (tức là hạnh phúc, các mối quan hệ giữa các cá nhân, chức năng hàng ngày cơ bản) bằng cách sử dụng thông tin về cả đối kháng và chức năng từ 3 nguồn - bản thân, người cung cấp thông tin và người phỏng vấn. Dữ liệu từ 318 người tham gia chính và người cung cấp thông tin. Chúng tôi trình bày 3 phát hiện chính: (1) Khi cả chức năng tâm lý xã hội và các đặc điểm đối kháng đều được người cung cấp thông tin đánh giá, thì tất cả các phạm vi khuyết tật tâm lý xã hội đều liên tục có liên quan tích cực với các đặc điểm đối kháng. (2) Tiếp theo, chúng tôi tạo một điểm số yếu tố khuyết tật tâm lý xã hội duy nhất thông qua phân tích các yếu tố chính của tất cả các điểm số chức năng tâm lý xã hội của người đánh giá. Khi báo cáo của cả 3 người đánh giá về sự đối kháng cấp độ miền được sử dụng làm các biến độc lập trong phân tích hồi quy đồng thời để dự đoán điểm số chức năng tổng thể này, thì sự đối kháng do người cung cấp thông tin báo cáo dự đoán chức năng tâm lý xã hội mạnh nhất, tiếp theo là sự đối kháng do người phỏng vấn đánh giá. (3) Sau đó, chúng tôi tạo 6 điểm số khía cạnh bằng cách tổng hợp điểm số của 3 người đánh giá trên mỗi khía cạnh. Khi chúng tôi sử dụng những điểm số này để dự đoán chức năng tâm lý xã hội, thì sự thù địch là đặc điểm chính dự đoán đáng kể chức năng tâm lý xã hội. Nghiên cứu cung cấp thêm thông tin chi tiết về mối liên quan của khuyết tật tâm lý xã hội với các khía cạnh đối kháng từ quan điểm của những người đánh giá khác nhau. Do đó, những phát hiện này giúp chúng ta hiểu sâu hơn về các kết quả tâm lý xã hội liên quan đến sự đối kháng, cốt lõi của các đặc điểm đen tối. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930026, "en": "Narcissism has significant interpersonal costs, yet little research has examined behavioral and affective patterns characteristic of narcissism in naturalistic settings. Here we studied the effect of narcissistic features on the dynamic processes of interpersonal behavior and affect in daily life. We used interpersonal theory to generate transactional models of social interaction (i.e., linkages among perceptions of others' behavior, affect, and one's own behavior) predicted to be characteristic of narcissism. Psychiatric outpatients (N = 102) completed clinical interviews and a 21-day ecological momentary assessment protocol using smartphones. After social interactions (N = 5,781), participants reported on perceptions of their interaction partner's behavior (scored along the dimensions of dominant-submissive and affiliative-quarrelsome), their own affect, and their own behavior. Multilevel structural equation modeling was used to examine dynamic links among behavior and affect across interactions, and the role of narcissism in moderating these links. Results showed that perceptions of others' dominance did not predict dominant behavior, but did predict quarrelsome behavior, and this link was potentiated by narcissism. Furthermore, the link between others' dominance and one's own quarrelsome behavior was mediated by negative affect. Moderated mediation was also found: Narcissism amplified the link between ratings of others' dominance and one's own quarrelsomeness and negative affect. Narcissism did not moderate the link between other dominance and own dominance, nor the link between other affiliation and own affiliation. These results suggest that narcissism is associated with specific interpersonal and affective processes, such that sensitivity to others' dominance triggers antagonistic behavior in daily life. (PsycINFO Database Record", "gemini": "Chủ nghĩa tự ái có chi phí quan hệ giữa các cá nhân đáng kể, nhưng ít nghiên cứu nào xem xét các mô hình hành vi và tình cảm đặc trưng của chủ nghĩa tự ái trong các bối cảnh tự nhiên. Ở đây, chúng tôi nghiên cứu tác động của các đặc điểm tự ái đối với các quá trình năng động của hành vi giữa các cá nhân và ảnh hưởng trong cuộc sống hàng ngày. Chúng tôi sử dụng lý thuyết giữa các cá nhân để tạo ra các mô hình giao dịch tương tác xã hội (tức là các mối liên kết giữa nhận thức về hành vi, tình cảm của người khác và hành vi của chính mình) được dự đoán là đặc trưng của chủ nghĩa tự ái. Bệnh nhân ngoại trú tâm thần (N = 102) đã hoàn thành các cuộc phỏng vấn lâm sàng và một giao thức đánh giá khoảnh khắc sinh thái trong 21 ngày bằng điện thoại thông minh. Sau các tương tác xã hội (N = 5.781), những người tham gia đã báo cáo về nhận thức của họ về hành vi của đối tác tương tác (được tính theo các chiều thống trị-thụ động và liên kết-cãi cọ), tình cảm của chính họ và hành vi của chính họ. Mô hình phương trình cấu trúc đa cấp được sử dụng để kiểm tra các liên kết động giữa hành vi và tình cảm trong các tương tác và vai trò của chủ nghĩa tự ái trong việc điều chỉnh các liên kết này. Kết quả cho thấy nhận thức về sự thống trị của người khác không dự đoán hành vi thống trị, nhưng dự đoán hành vi cãi cọ và mối liên hệ này được chủ nghĩa tự ái thúc đẩy. Hơn nữa, mối liên hệ giữa sự thống trị của người khác và hành vi cãi cọ của chính mình được trung gian bởi tình cảm tiêu cực. Sự hòa giải có điều kiện cũng được tìm thấy: Chủ nghĩa tự ái khuếch đại mối liên hệ giữa xếp hạng sự thống trị của người khác và sự cãi cọ và tình cảm tiêu cực của chính mình. Chủ nghĩa tự ái không điều chỉnh mối liên hệ giữa sự thống trị của người khác và sự thống trị của chính mình, cũng như mối liên hệ giữa sự liên kết của người khác và sự liên kết của chính mình. Những kết quả này cho thấy chủ nghĩa tự ái có liên quan đến các quá trình giữa các cá nhân và tình cảm cụ thể, chẳng hạn như sự nhạy cảm với sự thống trị của người khác gây ra hành vi đối kháng trong cuộc sống hàng ngày. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930027, "en": "A growing body of empirical evidence now supports a negative association between dark traits in leaders and the psychological health of employees. To date, such investigations have mostly focused on psychopathy, nonspecific measures of psychological wellbeing, and have not considered the mechanisms through which these relationships might operate. In the current study (N = 508), we utilized other-ratings of personality (employees rated leaders' personality), psychometrically robust measures, and sophisticated modeling techniques, to examine whether the effects of leaders' levels of narcissism and psychopathy on employee depression are mediated by workplace bullying. Structural equation models provided clear evidence to suggest that employee perceptions of both leader narcissism and psychopathy are associated with increased workplace bullying (25.8% and 41.0% variance explained, respectively) and that workplace bullying fully mediates the effect of leader narcissism and psychopathy on employee depression (21.5% and 20.8% variance explained, respectively). However, when psychopathy and narcissism were modeled concurrently, narcissism did not explain any variance in bullying, suggesting that it is the overlap between psychopathy and narcissism, namely, the \"dark core,\" which primarily accounts for the observed effects. We examined this assertion empirically and explored the unique effects of the subfactors of psychopathy. (PsycINFO Database Record", "gemini": "Một khối lượng ngày càng tăng các bằng chứng thực nghiệm hiện ủng hộ mối liên hệ tiêu cực giữa các đặc điểm đen tối ở những người lãnh đạo và sức khỏe tâm lý của nhân viên. Cho đến nay, các cuộc điều tra như vậy chủ yếu tập trung vào chứng thái nhân cách, các biện pháp không cụ thể về sức khỏe tâm lý và không xem xét các cơ chế mà các mối quan hệ này có thể hoạt động. Trong nghiên cứu hiện tại (N = 508), chúng tôi đã sử dụng các đánh giá tính cách của người khác (nhân viên đánh giá tính cách của người lãnh đạo), các biện pháp trắc nghiệm tâm lý mạnh mẽ và các kỹ thuật lập mô hình tinh vi để kiểm tra xem liệu các cấp độ tự ái và thái nhân cách của người lãnh đạo có ảnh hưởng đến nhân viên trầm cảm có được trung gian bởi bắt nạt nơi làm việc hay không. Các mô hình phương trình cấu trúc cung cấp bằng chứng rõ ràng cho thấy nhận thức của nhân viên về cả chứng tự ái và thái nhân cách của người lãnh đạo đều có liên quan đến việc bắt nạt nơi làm việc gia tăng (lần lượt giải thích được 25,8% và 41,0% phương sai) và việc bắt nạt nơi làm việc hoàn toàn trung gian tác động của chứng tự ái và thái nhân cách của người lãnh đạo đối với nhân viên trầm cảm (lần lượt giải thích được 21,5% và 20,8% phương sai). Tuy nhiên, khi thái nhân cách và tự ái được mô hình hóa đồng thời, tự ái không giải thích được bất kỳ phương sai nào trong việc bắt nạt, điều này cho thấy rằng chính sự chồng chéo giữa thái nhân cách và tự ái, cụ thể là \"lõi đen tối\", chủ yếu chịu trách nhiệm cho các tác động được quan sát thấy. Chúng tôi đã kiểm tra khẳng định này một cách thực nghiệm và khám phá các tác động riêng biệt của các yếu tố phụ của chứng thái nhân cách. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930028, "en": "Given the high-level impact of crime, empirical work is needed to identify personality traits, or overarching factors of personality pathology, that may leave individuals more prone to engage in criminal behaviors. This study empirically tested the distinction between the dark personality and vulnerable dark personality spectra (Miller et al., 2010) in a large offender sample and aimed to test how these personality factors relate to criminal dimensions and individual types of crime. Self-report and public record data verified criminal behaviors for 493 (34.7% women) adult offenders, and features of the 2 personality spectra were assessed using the Psychopathy Checklist: Screening Version, Narcissistic Personality Inventory-16, Hypersensitive Narcissism Scale, and Personality Assessment Inventory: Borderline Scale. Factor analyses supported the distinction between dark and vulnerable dark personality spectra and 3 dimensions of crime (i.e., crimes against property and persons, and drug-related crimes). Findings broadly pointed to trait vulnerabilities within the dark spectra (e.g., callousness) as relevant for criminal versatility and violent crimes against persons (e.g., assault, murder), and impulsive/reactive tendencies within the dark spectra (vulnerable dark) appeared to be particularly important for impulsive property crimes (e.g., theft, robbery) and drug offenses. Results may lend themselves well to refining offender intervention techniques and warrant further examination of the extent to which dark personality spectra coalesce as expected or similarly predict high-impact behaviors. (PsycINFO Database Record", "gemini": "Với tác động ở cấp độ cao của tội phạm, cần có công trình thực nghiệm để xác định các đặc điểm tính cách hoặc các yếu tố bao quát của bệnh lý nhân cách, có thể khiến các cá nhân dễ tham gia vào các hành vi phạm tội hơn. Nghiên cứu này đã kiểm tra thực nghiệm sự khác biệt giữa phổ tính cách đen tối và phổ tính cách đen tối dễ bị tổn thương (Miller et al., 2010) trong một mẫu tội phạm lớn và nhằm mục đích kiểm tra cách các yếu tố tính cách này liên quan đến các chiều hướng tội phạm và các loại tội phạm riêng lẻ. Dữ liệu tự báo cáo và hồ sơ công khai đã xác minh hành vi phạm tội đối với 493 (34,7% là phụ nữ) tội phạm trưởng thành và các đặc điểm của 2 phổ tính cách được đánh giá bằng Phiên bản sàng lọc Danh sách kiểm tra thái nhân cách, Bản kiểm kê tính cách tự ái-16, Thang tự ái quá mẫn cảm và Bản kiểm kê đánh giá tính cách: Thang ranh giới. Các phân tích nhân tố ủng hộ sự khác biệt giữa phổ tính cách đen tối và dễ bị tổn thương và 3 chiều hướng của tội phạm (tức là tội phạm chống lại tài sản và con người, và tội phạm liên quan đến ma túy). Các phát hiện chỉ ra một cách rộng rãi về các lỗ hổng về đặc điểm tính cách trong phổ đen tối (ví dụ: sự vô cảm) có liên quan đến tính linh hoạt của tội phạm và các tội bạo lực chống lại con người (ví dụ: tấn công, giết người) và các xu hướng bốc đồng/phản ứng trong phổ đen tối (dễ bị tổn thương) dường như đặc biệt quan trọng đối với các tội phạm về tài sản bốc đồng (ví dụ: trộm cắp, cướp) và các hành vi phạm tội liên quan đến ma túy. Kết quả có thể giúp cải thiện các kỹ thuật can thiệp của người phạm tội và bảo đảm xem xét thêm phạm vi mà các phổ tính cách đen tối hợp nhất như mong đợi hoặc dự đoán tương tự các hành vi có tác động cao. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930029, "en": "Studies have identified independent relationships between psychopathy, narcissism, and suicidality. The current study expands upon the extant literature by exploring psychopathic and narcissistic personality traits and components of the interpersonal-psychological theory of suicide, utilizing a 3-factor model of psychopathy and 2-factor model of pathological narcissism in community, undergraduate, and military individuals. We hypothesized that the impulsive-antisocial facets of psychopathy would be related to suicidal desire, whereas all facets of psychopathy would relate to the capability for suicide. We anticipated an association between pathological narcissism, thwarted belongingness, and capability for suicide, but not perceived burdensomeness. We further hypothesized a relationship between physical pain tolerance and persistence and the affective (i.e., callousness) facet of psychopathy. Results partially supported these hypotheses and underscore the need for further examination of these associations utilizing contemporary models of psychopathy and narcissism. (PsycINFO Database Record", "gemini": "Các nghiên cứu đã xác định mối quan hệ độc lập giữa chứng thái nhân cách, tự ái và hành vi tự tử. Nghiên cứu hiện tại mở rộng dựa trên các tài liệu hiện có bằng cách khám phá các đặc điểm tính cách thái nhân cách và tự ái và các thành phần của lý thuyết tâm lý giữa các cá nhân về hành vi tự tử, sử dụng mô hình 3 yếu tố về chứng thái nhân cách và mô hình 2 yếu tố về chứng tự ái bệnh lý trong cộng đồng, sinh viên đại học và quân nhân. Chúng tôi đưa ra giả thuyết rằng các khía cạnh bốc đồng-phản xã hội của chứng thái nhân cách sẽ liên quan đến mong muốn tự tử, trong khi tất cả các khía cạnh của chứng thái nhân cách sẽ liên quan đến khả năng tự tử. Chúng tôi dự đoán có mối liên hệ giữa chứng tự ái bệnh lý, sự cản trở về mặt tình cảm và khả năng tự tử, nhưng không phải là gánh nặng được nhận thức. Chúng tôi tiếp tục đưa ra giả thuyết về mối quan hệ giữa khả năng chịu đựng đau về thể chất và sự dai dẳng và khía cạnh tình cảm (tức là sự vô cảm) của chứng thái nhân cách. Kết quả ủng hộ một phần các giả thuyết này và nhấn mạnh sự cần thiết phải kiểm tra thêm các mối liên hệ này bằng các mô hình đương đại về chứng thái nhân cách và tự ái. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930030, "en": "The Dark Triad (DT) is a collection of overlapping aversive personality profiles constituting psychopathy, narcissism, and Machiavellianism. Debates remain regarding the optimal way to examine the unique outcomes associated with each construct, with several scholars advocating partialing these profiles in multiple regression analyses (i.e., removing their shared variance). The present paper details the pitfalls inherent in this approach by comparing the convergence and divergence of relations derived from raw and residualized DT composite scores. In Sample 1 (N = 393), DT scores were examined to determine the extent to which their raw and residualized components manifested similar relationships with the Five-Factor Model and the DSM-5 Section III personality disorder traits. In Sample 2 (N = 542), the same approach was taken in relation to an array of associated behaviors (e.g., antisocial behavior, promiscuity). Findings from Samples 1 and 2 demonstrate that the use of residualized (vs. raw) coefficients presents important interpretative challenges for both narcissism and Machiavellianism. This study illustrates the substantial interpretive difficulties that can arise when using findings from residualized analyses (e.g., multiple regression) to build nomological networks around Dark Triad constructs. We argue that bivariate relations be given preferential treatment, given their more direct ties to the assessments, and that if multivariate approaches are to be used, they must be accompanied by strong theory about the components of DT constructs. (PsycINFO Database Record", "gemini": "Bộ ba đen tối (DT) là tập hợp các cấu hình tính cách khó chịu chồng chéo tạo nên chứng thái nhân cách, tự ái và chủ nghĩa Machiavelli. Vẫn còn tranh luận về cách tối ưu để kiểm tra các kết quả duy nhất liên quan đến từng cấu trúc, với một số học giả ủng hộ việc phân loại các cấu hình này trong các phân tích hồi quy bội (tức là loại bỏ phương sai chung của chúng). Bài báo hiện tại trình bày chi tiết những cạm bẫy vốn có trong cách tiếp cận này bằng cách so sánh sự hội tụ và phân kỳ của các mối quan hệ có được từ điểm số tổng hợp DT thô và còn dư. Trong Mẫu 1 (N = 393), điểm DT được kiểm tra để xác định mức độ mà các thành phần thô và còn dư của chúng biểu hiện các mối quan hệ tương tự với Mô hình năm yếu tố và các đặc điểm rối loạn nhân cách Phần III của DSM-5. Trong Mẫu 2 (N = 542), cùng một cách tiếp cận đã được thực hiện liên quan đến một loạt các hành vi liên quan (ví dụ: hành vi chống đối xã hội, sự bừa bãi). Các phát hiện từ Mẫu 1 và 2 chứng minh rằng việc sử dụng các hệ số còn dư (so với thô) đặt ra những thách thức giải thích quan trọng đối với cả chứng tự ái và chủ nghĩa Machiavelli. Nghiên cứu này minh họa những khó khăn giải thích đáng kể có thể phát sinh khi sử dụng các phát hiện từ các phân tích còn dư (ví dụ: hồi quy bội) để xây dựng các mạng lưới danh nghĩa xung quanh các cấu trúc Bộ ba đen tối. Chúng tôi lập luận rằng các mối quan hệ theo cặp nên được ưu tiên, vì chúng có mối liên hệ trực tiếp hơn với các đánh giá và nếu sử dụng các phương pháp đa biến, thì chúng phải đi kèm với lý thuyết vững chắc về các thành phần của các cấu trúc DT. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930031, "en": "The past several decades have witnessed a proliferation of research on the dark triad (DT), a set of traits comprising Machiavellianism, narcissism, and psychopathy. The bulk of DT research has been marked by several core assumptions, most notably that each DT construct is a monolithic entity that is clearly separable from its counterpart DT constructs. To examine the tenability of these assumptions, we pooled data from 2 samples of North American community members (ns = 312 and 351) to explore (a) the external validity and profile similarities of DT indicators and (b) the factor structure of the DT. Using general personality dimensions as external criteria, we demonstrated that each DT measure is multidimensional and that subdimensions within DT measures often display sharply different and at times even opposing relations with personality domains; these opposing relations were largely obscured at the total score level adopted in most of the DT literature. In both samples, confirmatory factor analyses and exploratory structural equation models provided no clear support for the traditional tripartite DT structure delineated in the literature. Instead, various aspects of the DT constructs fractionated across a number of factors that represented more basic personality elements (e.g., emotional stability, grandiosity). Taken together, our findings raise serious questions regarding the standard model of DT research and suggest that the questions posed regarding the correlates of DT constructs hinge crucially on the specific DT measure and subdimension examined. (PsycINFO Database Record", "gemini": "Vài thập kỷ qua đã chứng kiến sự gia tăng nhanh chóng của các nghiên cứu về bộ ba đen tối (DT), một tập hợp các đặc điểm bao gồm chủ nghĩa Machiavelli, tự ái và thái nhân cách. Phần lớn các nghiên cứu về DT được đánh dấu bằng một số giả định cốt lõi, đáng chú ý nhất là mỗi cấu trúc DT là một thực thể thống nhất có thể tách biệt rõ ràng khỏi các cấu trúc DT đối tác của nó. Để kiểm tra tính khả thi của các giả định này, chúng tôi đã tập hợp dữ liệu từ 2 mẫu thành viên cộng đồng Bắc Mỹ (ns = 312 và 351) để khám phá (a) tính hợp lệ bên ngoài và các điểm tương đồng về cấu hình của các chỉ số DT và (b) cấu trúc nhân tố của DT. Sử dụng các chiều tính cách chung làm tiêu chuẩn bên ngoài, chúng tôi đã chứng minh rằng mỗi biện pháp DT là đa chiều và các chiều phụ trong các biện pháp DT thường biểu hiện các mối quan hệ hoàn toàn khác biệt và đôi khi thậm chí đối lập với các phạm vi tính cách; các mối quan hệ đối lập này phần lớn không được đề cập đến ở cấp độ điểm số tổng thể được áp dụng trong hầu hết các tài liệu về DT. Trong cả hai mẫu, các phân tích nhân tố xác nhận và các mô hình phương trình cấu trúc thăm dò đều không cung cấp sự hỗ trợ rõ ràng cho cấu trúc DT ba thành phần truyền thống được mô tả trong tài liệu. Thay vào đó, các khía cạnh khác nhau của các cấu trúc DT được phân chia thành một số yếu tố đại diện cho các yếu tố tính cách cơ bản hơn (ví dụ: sự ổn định về mặt cảm xúc, sự vĩ đại). Xét về tổng thể, những phát hiện của chúng tôi đặt ra những câu hỏi nghiêm trọng liên quan đến mô hình tiêu chuẩn của nghiên cứu DT và gợi ý rằng các câu hỏi được đặt ra liên quan đến mối tương quan của các cấu trúc DT phụ thuộc rất nhiều vào biện pháp DT cụ thể và chiều phụ được kiểm tra. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930032, "en": "Network analysis is quickly gaining popularity in psychopathology research as a method that aims to reveal causal relationships among individual symptoms. To date, 4 main types of psychopathology networks have been proposed: (a) association networks, (b) regularized concentration networks, (c) relative importance networks, and (d) directed acyclic graphs. The authors examined the replicability of these analyses based on symptoms of major depression and generalized anxiety between and within 2 highly similar epidemiological samples (i.e., the National Comorbidity Survey-Replication [n = 9282] and the National Survey of Mental Health and Wellbeing [n = 8841]). Although association networks were stable, the 3 other types of network analysis (i.e., the conditional independence networks) had poor replicability between and within methods and samples. The detailed aspects of the models-such as the estimation of specific edges and the centrality of individual nodes-were particularly unstable. For example, 44% of the symptoms were estimated as the \"most influential\" on at least 1 centrality index across the 6 conditional independence networks in the full samples, and only 13-21% of the edges were consistently estimated across these networks. One of the likely reasons for the instability of the networks is the predominance of measurement error in the assessment of individual symptoms. The authors discuss the implications of these findings for the growing field of psychopathology network research, and conclude that novel results originating from psychopathology networks should be held to higher standards of evidence before they are ready for dissemination or implementation in the field. (PsycINFO Database Record", "gemini": "Phân tích mạng nhanh chóng trở nên phổ biến trong nghiên cứu về bệnh lý tâm thần như một phương pháp nhằm mục đích tiết lộ các mối quan hệ nhân quả giữa các triệu chứng riêng lẻ. Cho đến nay, 4 loại mạng bệnh lý tâm thần chính đã được đề xuất: (a) mạng liên kết, (b) mạng tập trung chính quy, (c) mạng tầm quan trọng tương đối và (d) đồ thị không có chu trình hướng. Các tác giả đã kiểm tra khả năng sao chép của các phân tích này dựa trên các triệu chứng của chứng trầm cảm chính và lo âu lan tỏa giữa và trong 2 mẫu dịch tễ học rất giống nhau (tức là Khảo sát dịch tễ học quốc gia - Bản sao [n = 9282] và Khảo sát quốc gia về sức khỏe tâm thần và hạnh phúc [n = 8841]). Mặc dù các mạng liên kết ổn định, nhưng 3 loại phân tích mạng khác (tức là các mạng độc lập có điều kiện) có khả năng sao chép kém giữa các phương pháp và mẫu. Các khía cạnh chi tiết của các mô hình - chẳng hạn như ước tính các cạnh cụ thể và vị trí trung tâm của các nút riêng lẻ - đặc biệt không ổn định. Ví dụ, 44% các triệu chứng được ước tính là \"có ảnh hưởng nhất\" trên ít nhất 1 chỉ số trung tâm trong số 6 mạng độc lập có điều kiện trong các mẫu đầy đủ và chỉ có 13-21% các cạnh được ước tính nhất quán trên các mạng này. Một trong những lý do có thể xảy ra tình trạng không ổn định của các mạng là lỗi đo lường chiếm ưu thế trong việc đánh giá các triệu chứng riêng lẻ. Các tác giả thảo luận về ý nghĩa của những phát hiện này đối với lĩnh vực nghiên cứu mạng bệnh lý tâm thần đang phát triển và kết luận rằng các kết quả mới có nguồn gốc từ các mạng bệnh lý tâm thần nên được áp dụng các tiêu chuẩn bằng chứng cao hơn trước khi chúng sẵn sàng để phổ biến hoặc triển khai trong lĩnh vực này. (Hồ sơ cơ sở dữ liệu PsycINFO"} +{"idx": 1930033, "en": "Forbes, Wright, Markon, and Krueger (2017) stated that \"psychopathology networks have limited replicability\" (p. 1011) and that \"popular network analysis methods produce unreliable results\" (p. 1011). These conclusions are based on an assessment of the replicability of four different network models for symptoms of major depression and generalized anxiety across two samples; in addition, Forbes et al. analyzed the stability of the network models within the samples using split-halves. Our reanalysis of the same data with the same methods led to results directly opposed to theirs: All network models replicated very well across the two data sets and across the split-halves. We trace the differences between Forbes et al.'s results and our own to the fact that they did not appear to accurately implement all network models and used debatable metrics to assess replicability. In particular, they deviated from existing estimation routines for relative importance networks, did not acknowledge the fact that the skip structure used in the interviews strongly distorted correlations between symptoms, and incorrectly assumed that network structures and metrics should be the same not only across the different samples but also across the different network models used. In addition to a comprehensive reanalysis of the data, we end with a discussion of best practices concerning future research into the replicability of psychometric networks. (PsycINFO Database Record", "gemini": "Forbes, Wright, Markon và Krueger (2017) đã tuyên bố rằng \"các mạng bệnh lý tâm thần có khả năng tái tạo hạn chế\" (trang 1011) và \"các phương pháp phân tích mạng phổ biến tạo ra các kết quả không đáng tin cậy\" (trang 1011). Những kết luận này dựa trên đánh giá về khả năng tái tạo của bốn mô hình mạng khác nhau đối với các triệu chứng của bệnh trầm cảm nặng và rối loạn lo âu toàn thể trên hai mẫu; ngoài ra, Forbes et al. đã phân tích tính ổn định của các mô hình mạng trong các mẫu bằng cách chia đôi. Phân tích lại cùng một dữ liệu với cùng phương pháp của chúng tôi dẫn đến kết quả trái ngược hoàn toàn với kết quả của họ: Tất cả các mô hình mạng đều được sao chép rất tốt trên hai tập dữ liệu và trên các phần chia đôi. Chúng tôi theo dõi sự khác biệt giữa kết quả của Forbes et al. và kết quả của chúng tôi là do họ dường như không thực hiện chính xác tất cả các mô hình mạng và sử dụng các số liệu có thể tranh cãi để đánh giá khả năng tái tạo. Cụ thể, họ đã đi chệch khỏi các quy trình ước tính hiện có đối với các mạng có tầm quan trọng tương đối, không thừa nhận thực tế là cấu trúc bỏ qua được sử dụng trong các cuộc phỏng vấn làm bóp méo mạnh mẽ các mối tương quan giữa các triệu chứng và giả định không chính xác rằng các cấu trúc và số liệu mạng không chỉ giống nhau trên các mẫu khác nhau mà còn trên các mô hình mạng khác nhau được sử dụng. Ngoài việc phân tích lại toàn diện dữ liệu, chúng tôi kết thúc bằng một cuộc thảo luận về các thông lệ tốt nhất liên quan đến nghiên cứu trong tương lai về khả năng tái tạo của các mạng trắc nghiệm tâm lý. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930034, "en": "Forbes, Wright, Markon, and Krueger (2017) make a compelling case for proceeding cautiously with respect to the overinterpretation and dissemination of results using the increasingly popular approach of creating \"networks\" from co-occurrences of psychopathology symptoms. We commend the authors on their initial investigation and their utilization of cross-validation techniques in an effort to capture the stability of a variety of network estimation methods. Such techniques get at the heart of establishing \"reproducibility,\" an increasing focus of concern in both psychology (e.g., Pashler & Wagenmakers, 2012) and science more generally (e.g., Baker, 2016). However, as we will show, the problem is likely worse (or at least more complicated) than they initially indicated. Specifically, for multivariate binary data, the marginal distributions enforce a large degree of structure on the data. We show that some expected measurements-such as commonly used centrality statistics-can have substantially higher values than what would usually be expected. As such, we propose a nonparametric approach to generate confidence intervals through Monte Carlo simulation. We apply the proposed methodology to the National Comorbidity Survey - Replication, provided by Forbes et al., finding that the many of the results are indistinguishable from what would be expected by chance. Further, we discuss the problem of multiple testing and potential issues of applying methods developed for 1-mode networks (e.g., ties within a single set of observations) to 2-mode networks (e.g., ties between 2 distinct sets of entities). When taken together, these issues indicate that the psychometric network models should be employed with extreme caution and interpreted guardedly. (PsycINFO Database Record", "gemini": "Forbes, Wright, Markon và Krueger (2017) đưa ra một trường hợp hấp dẫn để tiến hành thận trọng đối với việc giải thích quá mức và phổ biến kết quả bằng cách sử dụng cách tiếp cận ngày càng phổ biến là tạo \"mạng\" từ các triệu chứng bệnh lý tâm thần đồng thời xảy ra. Chúng tôi khen ngợi các tác giả về cuộc điều tra ban đầu của họ và việc sử dụng các kỹ thuật xác thực chéo trong nỗ lực nắm bắt tính ổn định của nhiều phương pháp ước tính mạng khác nhau. Các kỹ thuật như vậy đi vào trọng tâm của việc thiết lập \"khả năng tái tạo\", một mối quan tâm ngày càng được quan tâm trong cả tâm lý học (ví dụ: Pashler & Wagenmakers, 2012) và khoa học nói chung (ví dụ: Baker, 2016). Tuy nhiên, như chúng tôi sẽ trình bày, vấn đề có thể tồi tệ hơn (hoặc ít nhất là phức tạp hơn) so với những gì họ chỉ ra ban đầu. Cụ thể, đối với dữ liệu nhị phân đa biến, các phân phối biên áp đặt một cấu trúc lớn trên dữ liệu. Chúng tôi chỉ ra rằng một số phép đo dự kiến - chẳng hạn như thống kê trung tâm thường được sử dụng - có thể có giá trị cao hơn đáng kể so với những gì thường được mong đợi. Do đó, chúng tôi đề xuất một phương pháp phi tham số để tạo các khoảng tin cậy thông qua mô phỏng Monte Carlo. Chúng tôi áp dụng phương pháp được đề xuất vào Khảo sát mắc bệnh toàn quốc - Bản sao do Forbes et al. cung cấp, thấy rằng nhiều kết quả không thể phân biệt được với những gì có thể xảy ra ngẫu nhiên. Hơn nữa, chúng tôi thảo luận về vấn đề kiểm tra nhiều lần và các vấn đề tiềm ẩn khi áp dụng các phương pháp được phát triển cho các mạng 1 chế độ (ví dụ: mối quan hệ trong một tập hợp các quan sát duy nhất) vào các mạng 2 chế độ (ví dụ: mối quan hệ giữa 2 tập thực thể riêng biệt). Khi được kết hợp lại, những vấn đề này chỉ ra rằng các mô hình mạng trắc nghiệm tâm lý nên được sử dụng cực kỳ thận trọng và được diễn giải một cách thận trọng. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930035, "en": "In our target article, we tested the replicability of 4 popular psychopathology network estimation methods that aim to reveal causal relationships among symptoms of mental illness. We started with the focal data set from the 2 foundational psychopathology network papers (i.e., the National Comorbidity Survey-Replication) and identified the National Survey of Mental Health and Wellbeing as a close methodological match for comparison. We compared the psychopathology networks estimated in each data set-as well as in 10 sets of random split-halves within each data set-with the goal of quantifying the replicability of the network parameters as they are interpreted in the extant psychopathology network literature. We concluded that current psychopathology network methods have limited replicability both within and between samples and thus have limited utility. Here we respond to the 2 commentaries on our target article, concluding that the findings of Steinley, Hoffman, Brusco, and Sher (2017)-along with other recent developments in the literature-provide further conclusive evidence that psychopathology networks have poor replicability and utility. (PsycINFO Database Record", "gemini": "Trong bài báo mục tiêu của mình, chúng tôi đã kiểm tra khả năng tái tạo của 4 phương pháp ước tính mạng bệnh lý tâm thần phổ biến nhằm mục đích tiết lộ các mối quan hệ nhân quả giữa các triệu chứng của bệnh tâm thần. Chúng tôi bắt đầu với tập dữ liệu trọng tâm từ 2 bài báo về mạng bệnh lý tâm thần nền tảng (tức là Khảo sát mắc bệnh toàn quốc - Bản sao) và xác định Khảo sát toàn quốc về sức khỏe và hạnh phúc tâm thần là một phương pháp phù hợp chặt chẽ để so sánh. Chúng tôi đã so sánh các mạng bệnh lý tâm thần được ước tính trong mỗi tập dữ liệu - cũng như trong 10 tập các phần chia đôi ngẫu nhiên trong mỗi tập dữ liệu - với mục tiêu định lượng khả năng tái tạo của các tham số mạng khi chúng được diễn giải trong các tài liệu về mạng bệnh lý tâm thần hiện có. Chúng tôi kết luận rằng các phương pháp mạng bệnh lý tâm thần hiện tại có khả năng tái tạo hạn chế cả trong và giữa các mẫu và do đó có tiện ích hạn chế. Tại đây, chúng tôi trả lời 2 bình luận về bài báo mục tiêu của mình, kết luận rằng những phát hiện của Steinley, Hoffman, Brusco và Sher (2017) - cùng với các diễn biến gần đây khác trong tài liệu - cung cấp thêm bằng chứng kết luận rằng các mạng bệnh lý tâm thần có khả năng tái tạo và tiện ích kém. (Bản ghi cơ sở dữ liệu PsycINFO"} +{"idx": 1930036, "en": "Amyloidosis of the gastrointestinal tract is usually a systemic disease. Localized gastrointestinal amyloidosis without evidence of extraintestinal involvement or an associated plasma cell dyscrasia is uncommon and does not usually cause death. We report a case of a patient with localized gastrointestinal amyloidosis who presented with protein-losing enteropathy and a fatal upper gastrointestinal bleed.", "gemini": "Bệnh amyloidosis đường tiêu hóa thường là một bệnh toàn thân. Bệnh amyloidosis đường tiêu hóa khu trú không có bằng chứng về tình trạng liên quan ngoài ruột hoặc loạn sản tế bào plasma liên quan không phổ biến và thường không gây tử vong. Chúng tôi báo cáo một trường hợp bệnh nhân bị bệnh amyloidosis đường tiêu hóa khu trú biểu hiện bằng bệnh lý mất protein đường ruột và chảy máu đường tiêu hóa trên gây tử vong."} +{"idx": 1930037, "en": "Primary hyperoxaluria (PH) is a metabolic liver disease with an autosomal recessive inheritance that results in oxalate overproduction that cannot be metabolized by the liver. Urinary excretion of oxalate results in lithiasis and nephrocalcinosis leading to a progressive loss of renal function that often requires renal replacement therapy despite medical treatment. Type 1 PH is the most common form and is due to a deficiency in the alanine-glycolate aminotransferase enzyme found in hepatic peroxisomes. Therefore, a liver-kidney simultaneous transplant (LKST) is the definitive treatment for end-stage renal disease (ESRD) patients. However, some studies suggest that the morbidity and mortality rates are greater when this procedure is performed instead of only a kidney transplant (IKT). Herein, we report five patients with PH and a mean glomerular filtration rate of 20.2 ± 1.3 ml/min/1.73 m2 who received a LKST between 1999 and 2015 at the Hospital Universitario 12 de Octubre. Recurrence and liver or kidney graft loss was not observed during the postoperative period and only one case of late acute rejection without graft loss was diagnosed. The recipient survival rate was 100% with a median follow up of 84 months. As LKST is a curative and safe procedure with a low mortality and high survival rate, it must be considered as the treatment of choice in adults with HP and ESRD.", "gemini": "Tăng oxalat niệu nguyên phát (PH) là bệnh gan chuyển hóa có tính trạng di truyền lặn trên nhiễm sắc thể thường, dẫn đến tình trạng gan không chuyển hóa được oxalat dư thừa. Oxalat bài tiết qua nước tiểu dẫn đến sỏi và lắng canxi ở thận, gây suy giảm chức năng thận tiến triển, thường phải thay thế thận mặc dù đã điều trị nội khoa. PH týp 1 là dạng phổ biến nhất và là do thiếu men alanine-glycolate aminotransferase có trong peroxisome ở gan. Do đó, ghép đồng thời gan-thận (LKST) là phương pháp điều trị dứt điểm cho bệnh nhân suy thận giai đoạn cuối (ESRD). Tuy nhiên, một số nghiên cứu cho thấy tỷ lệ mắc bệnh và tử vong cao hơn khi thực hiện thủ thuật này thay vì chỉ ghép thận (IKT). Trong bài báo này, chúng tôi báo cáo năm bệnh nhân bị PH và tốc độ lọc cầu thận trung bình là 20,2 ± 1,3 ml/phút/1,73 m2, đã được ghép LKST từ năm 1999 đến 2015 tại Bệnh viện Universitario 12 de Octubre. Không quan sát thấy tình trạng tái phát và mất ghép gan hoặc thận trong thời gian hậu phẫu và chỉ chẩn đoán được một trường hợp thải ghép cấp muộn mà không mất ghép. Tỷ lệ sống của người nhận là 100% với thời gian theo dõi trung bình là 84 tháng. Vì LKST là một thủ thuật an toàn và có khả năng chữa khỏi với tỷ lệ tử vong thấp và tỷ lệ sống cao, nên phải coi đây là phương pháp điều trị lựa chọn ở người lớn bị HP và ESRD."} +{"idx": 1930038, "en": "BACKGROUND We have recently demonstrated the feasibility of lengthening the duodenum in children with short bowel syndrome and a dilated duodenum. This procedure gains additional intestinal length in a challenging area of autologous gut reconstruction. Herein, we report the successful application of this technique in an adult with ultra-short bowel syndrome. CASE REPORT A 25-year-old man with a history of mid-gut volvulus was referred to our center for intestinal transplant evaluation. Only a megaduodenum stump that reached as far as the third portion (30 cm of length) and the colon up to the hepatic flexure in the form of a mucous fistula was retained. A gastrostomy tube drained gastric and bilio-pancreatic secretions (output range: 2.5-4 liters/day). The time spent on parenteral nutrition (3 liters/day; 1500 calories/day) and I.V. fluid (1.5-2 liters/day) administration was 24 hours per day. The patient underwent duodenal lengthening and tapering with 7 sequential transverse applications (5 of 45 mm and 2 of 60 mm) of an endoscopic stapler on the anterior and posterior walls of the duodenum, respecting the pancreatic parenchyma and end-to-side duodeno-colonic anastomosis. The final duodenal length was 83 cm. The pre-lengthening citrulline level increased from 13.6 micromol/L to 21.6 micromol/L one year post-lengthening. After 24 month of follow-up, the time on a parenteral pump was shortened to 9 hours during the night. The volume and calorie requirements were also reduced by half. DISCUSSION Duodenal lengthening may be effective as part of the autologous intestinal reconstruction armamentarium in adults with short bowel syndrome.", "gemini": "LÝ LỊCH GẦN ĐÂY Chúng tôi đã chứng minh tính khả thi của việc kéo dài tá tràng ở trẻ em mắc hội chứng ruột ngắn và tá tràng giãn. Thủ thuật này giúp kéo dài thêm chiều dài ruột trong một khu vực khó khăn để tái tạo ruột tự thân. Trong bài báo này, chúng tôi báo cáo việc ứng dụng thành công kỹ thuật này ở một người lớn mắc hội chứng ruột cực ngắn. BÁO CÁO CA BỆNH Một nam thanh niên 25 tuổi có tiền sử bị lồng ruột giữa đã được chuyển đến trung tâm của chúng tôi để đánh giá ghép ruột. Chỉ còn lại một đoạn tá tràng khổng lồ dài đến phần ba (dài 30 cm) và đại tràng lên đến góc gan dưới dạng lỗ rò niêm mạc. Một ống thông dạ dày dẫn lưu dịch tiết dạ dày và mật tụy (lượng dịch dẫn lưu: 2,5-4 lít/ngày). Thời gian dùng dinh dưỡng qua đường tĩnh mạch (3 lít/ngày; 1500 calo/ngày) và truyền dịch I.V. (1,5-2 lít/ngày) là 24 giờ mỗi ngày. Bệnh nhân đã được kéo dài và thu nhỏ tá tràng bằng 7 lần đặt máy khâu nội soi theo chiều ngang tuần tự (5 lần đặt máy khâu 45 mm và 2 lần đặt máy khâu 60 mm) ở thành trước và thành sau của tá tràng, tôn trọng nhu mô tụy và thực hiện nối tá tràng-đại tràng tận-bên. Chiều dài tá tràng cuối cùng là 83 cm. Nồng độ citrulline trước khi kéo dài tăng từ 13,6 micromol/L lên 21,6 micromol/L một năm sau khi kéo dài. Sau 24 tháng theo dõi, thời gian sử dụng máy bơm tĩnh mạch giảm xuống còn 9 giờ vào ban đêm. Lượng thể tích và nhu cầu calo cũng giảm một nửa. THẢO LUẬN Kéo dài tá tràng có thể có hiệu quả như một phần của phương tiện tái tạo ruột tự thân ở người lớn mắc hội chứng ruột ngắn."} +{"idx": 1930039, "en": "BACKGROUND AND AIM Preoperative endoscopic tattooing is an effective procedure to identify small intraoperative neoplasms. However, there are no defined criteria with regard to the indications for endoscopic tattooing of these lesions at the time of diagnosis. The aim of this study was to establish endoscopic criteria that allow the selection of patients who will need a tattoo during the diagnostic colonoscopy. METHODS An ambispective study of patients undergoing laparoscopy due to a colorectal neoplasia who underwent endoscopic tattooing during the period from 2007-2013 and 2016-2017. According to the endoscopic description of the neoplasms, the classification was polypoid lesions, neoplasms occupying < 50% or ≥ 50% of the intestinal lumen and stenosing neoplasias. RESULTS Tattooing of the lesion was performed in 120 patients and the same lesions were identified during surgery in 114 (95%) cases. Most of the neoplasias described as polypoids and neoplasias that occupied < 50% of the intestinal lumen were not visualized during surgery and therefore required a tattoo (33 of 42 and 18 of 26 respectively, p = 0.0001, X2). On the other hand, stenosing lesions or neoplasias occupying ≥ 50% of the intestinal lumen were mostly identified during surgery (15 of 15 and 36 of 37 respectively, p = 0.0001, X2) without the need for a tattoo. Overall, the identification of neoplasms according to established criteria was 98%. CONCLUSION These results suggest that it is possible to establish endoscopic criteria that allow a successful selective tattooing during diagnostic endoscopy.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Xăm nội soi trước phẫu thuật là một thủ thuật hiệu quả để xác định các khối u nhỏ trong khi phẫu thuật. Tuy nhiên, không có tiêu chuẩn xác định nào liên quan đến chỉ định xăm nội soi các tổn thương này tại thời điểm chẩn đoán. Mục đích của nghiên cứu này là thiết lập các tiêu chuẩn nội soi cho phép lựa chọn những bệnh nhân cần xăm trong quá trình nội soi đại tràng chẩn đoán. PHƯƠNG PHÁP Một nghiên cứu hồi cứu đối với những bệnh nhân nội soi ổ bụng do ung thư đại trực tràng đã được xăm nội soi trong giai đoạn từ 2007-2013 và 2016-2017. Theo mô tả nội soi của các khối u, phân loại là tổn thương polyp, khối u chiếm < 50% hoặc ≥ 50% lòng ruột và khối u hẹp. KẾT QUẢ Xăm tổn thương đã được thực hiện ở 120 bệnh nhân và các tổn thương tương tự đã được xác định trong quá trình phẫu thuật ở 114 (95%) trường hợp. Hầu hết các khối u được mô tả là polyp và các khối u chiếm < 50% lòng ruột không được nhìn thấy trong quá trình phẫu thuật và do đó cần phải xăm (lần lượt là 33 trong số 42 và 18 trong số 26, p = 0,0001, X2). Mặt khác, các tổn thương hẹp hoặc khối u chiếm ≥ 50% lòng ruột chủ yếu được xác định trong quá trình phẫu thuật (lần lượt là 15 trong số 15 và 36 trong số 37, p = 0,0001, X2) mà không cần xăm. Nhìn chung, việc xác định các khối u theo các tiêu chuẩn đã thiết lập là 98%. KẾT LUẬN Các kết quả này cho thấy có thể thiết lập các tiêu chuẩn nội soi cho phép xăm chọn lọc thành công trong nội soi chẩn đoán."} +{"idx": 1930040, "en": "INTRODUCTION Benign biliary diseases are traditionally treated using plastic stents. However, fully covered self-expanding metal stents are currently gaining acceptance for the treatment of these pathologies. OBJECTIVE To assess the effectiveness and complications associated with the placement of temporary endoscopic fully covered self-expanding metal stents for the treatment of benign biliary diseases. MATERIALS AND METHODS This was a retrospective and observational study using a prospective database from a tertiary care center from March 2012 to May 2016. Some patients that had a fully covered metal stent due to a benign biliary disease were also included. The indications, resolution, technical success rates and adverse events were documented. RESULTS 31 patients were included with a total of 34 stents inserted. The indications were as follows: 8 (25%) post cholecystectomy biliary stenoses, 11 (31%) large or multiple choledocholithiasis, 3 (8.3%) biliary fistulas, 2 (6%) post-liver transplant stenoses, 2 (8.3%) papillary stenoses, 2 (6%) perforations and 2 (6%) bleeds. The global resolution success rate of the stents for all pathologies was 88%, this included 87.5% (7/8) in post cholecystectomy stenoses, 73% (8/11) in large choledocholithiasis and 100% for the remaining indications. Thirty-three of 34 stents were removed after an average of 133 days (ranging from 10 to 180 days). No complications were registered. CONCLUSION Fully covered self-expanding metal stents are an effective and reliable alternative for the resolution of benign biliary diseases.", "gemini": "GIỚI THIỆU Các bệnh lý đường mật lành tính thường được điều trị bằng stent nhựa. Tuy nhiên, stent kim loại tự nở hoàn toàn phủ hiện đang được chấp nhận để điều trị các bệnh lý này. MỤC TIÊU Đánh giá hiệu quả và các biến chứng liên quan đến việc đặt stent kim loại tự nở hoàn toàn nội soi tạm thời để điều trị các bệnh lý đường mật lành tính. VẬT LIỆU VÀ PHƯƠNG PHÁP Đây là một nghiên cứu hồi cứu và quan sát sử dụng cơ sở dữ liệu triển vọng từ một trung tâm chăm sóc sức khỏe cấp ba từ tháng 3 năm 2012 đến tháng 5 năm 2016. Một số bệnh nhân đã đặt stent kim loại phủ hoàn toàn do bệnh đường mật lành tính cũng được đưa vào. Các chỉ định, độ phân giải, tỷ lệ thành công về mặt kỹ thuật và các tác dụng phụ đã được ghi lại. KẾT QUẢ 31 bệnh nhân được đưa vào với tổng số 34 stent được đặt. Các chỉ định như sau: 8 (25%) hẹp đường mật sau cắt túi mật, 11 (31%) sỏi ống mật chủ lớn hoặc nhiều, 3 (8,3%) lỗ rò đường mật, 2 (6%) hẹp sau ghép gan, 2 (8,3%) hẹp nhú, 2 (6%) thủng và 2 (6%) chảy máu. Tỷ lệ thành công phân giải toàn cầu của stent đối với tất cả các bệnh lý là 88%, bao gồm 87,5% (7/8) ở hẹp sau cắt túi mật, 73% (8/11) ở sỏi ống mật chủ lớn và 100% đối với các chỉ định còn lại. Ba mươi ba trong số 34 stent đã được tháo ra sau trung bình 133 ngày (dao động từ 10 đến 180 ngày). Không có biến chứng nào được ghi nhận. KẾT LUẬN Stent kim loại tự nở hoàn toàn phủ là một phương pháp thay thế hiệu quả và đáng tin cậy để giải quyết các bệnh lý đường mật lành tính."} +{"idx": 1930041, "en": "On May 22, 2017, administrative law Judge Leslie Rogall of the Department of Health and Human Services' Departmental Appeals Board, Civil Remedies Division, ruled in favor of the Office of Research Integrity (ORI) concerning its decision to charge former University of California at Riverside biochemistry professor Frank Sauer with research misconduct for fabricating or falsifying digital image data included in three papers and seven grant applications submitted to the National Institutes of Health. More specifically, Sauer was deemed responsible for manipulating, reusing, and falsely labeling images of autoradiograms and gels in his research in epigenetics. One month after this decision, ORI announced its final ruling concerning Sauer, which barred him from serving in any advisory capacity to the Public Health Services and required him to retract affected papers. The case raises some interesting and important questions concerning research integrity because it focused on the legal issue of what constitutes recklessness in scientific research.", "gemini": "Vào ngày 22 tháng 5 năm 2017, Thẩm phán luật hành chính Leslie Rogall của Ban kháng cáo của Bộ Y tế và Dịch vụ Nhân sinh, Bộ phận Biện pháp khắc phục Dân sự, đã ra phán quyết có lợi cho Văn phòng Đạo đức Nghiên cứu (ORI) liên quan đến quyết định buộc tội cựu giáo sư sinh hóa Frank Sauer của Đại học California tại Riverside về hành vi sai trái trong nghiên cứu vì đã chế tạo hoặc làm giả dữ liệu hình ảnh kỹ thuật số có trong ba bài báo và bảy đơn xin trợ cấp được gửi tới Viện Y tế Quốc gia. Cụ thể hơn, Sauer bị coi là chịu trách nhiệm chỉnh sửa, sử dụng lại và dán nhãn sai hình ảnh của các bản tự chụp và gel trong nghiên cứu của ông về biểu sinh. Một tháng sau quyết định này, ORI đã công bố phán quyết cuối cùng liên quan đến Sauer, trong đó cấm ông phục vụ bất kỳ chức vụ cố vấn nào cho Dịch vụ Y tế Công cộng và yêu cầu ông rút lại các bài báo bị ảnh hưởng. Vụ việc đặt ra một số câu hỏi thú vị và quan trọng liên quan đến tính toàn vẹn của nghiên cứu vì nó tập trung vào vấn đề pháp lý về những gì cấu thành sự liều lĩnh trong nghiên cứu khoa học."} +{"idx": 1930042, "en": "In response to the potential hazards associated with the globalization of the food industry, research has been focused on the development of new sensing techniques to provide the means of contamination detection at any stage in the food supply chain. The demand for on-site detection is growing as pre-emptive sensing of pathogens could eliminate foodborne-related outbreaks and associated healthcare costs. Reduction in food waste is also a driver for point-of-use (POU) sensing, from both an economic and environmental standpoint. The following review discusses the latest advancements in platforms that have the greatest potential for inexpensive, real-time detection, and identification of foodborne pathogens. Specific focus has been placed on the development techniques, which utilize micro- and nanoscale technology. Sample preparation-free techniques are also discussed, as the growing demand to enable POU sensing at any stage in the food supply chain will be a major driver toward the advancements of these nondestructive methods.", "gemini": "Để ứng phó với các mối nguy tiềm ẩn liên quan đến quá trình toàn cầu hóa của ngành công nghiệp thực phẩm, các nghiên cứu đã tập trung vào việc phát triển các kỹ thuật cảm biến mới để cung cấp phương tiện phát hiện ô nhiễm ở bất kỳ giai đoạn nào trong chuỗi cung ứng thực phẩm. Nhu cầu phát hiện tại chỗ đang tăng lên vì việc phát hiện mầm bệnh trước có thể loại bỏ các đợt bùng phát liên quan đến thực phẩm và các chi phí chăm sóc sức khỏe liên quan. Giảm lãng phí thực phẩm cũng là động lực thúc đẩy cảm biến tại điểm sử dụng (POU), cả về mặt kinh tế và môi trường. Đánh giá sau đây thảo luận về những tiến bộ mới nhất trong các nền tảng có tiềm năng lớn nhất để phát hiện và xác định mầm bệnh do thực phẩm gây ra theo thời gian thực và không tốn kém. Trọng tâm cụ thể đã được đặt vào các kỹ thuật phát triển, sử dụng công nghệ vi mô và nano. Các kỹ thuật không cần chuẩn bị mẫu cũng được thảo luận, vì nhu cầu ngày càng tăng đối với khả năng cảm biến POU ở bất kỳ giai đoạn nào trong chuỗi cung ứng thực phẩm sẽ là động lực chính thúc đẩy sự tiến bộ của các phương pháp không phá hủy này."} +{"idx": 1930043, "en": "Salmonella Enteritidis remains a standout among the leading causes of foodborne diseases worldwide. Previous studies have demonstrated that a unique clonal group of Salmonella Enteritidis, named SE86, is involved in foodborne outbreaks in southern Brazil and is frequently identified among strains isolated from poultry. The aim of this study was to determine the influence of the isolation source (food products involved in salmonellosis outbreaks and poultry sources) on the phenotypic and molecular characteristics of Salmonella Enteritidis SE86. A biofilm formation assay, antimicrobial susceptibility test, polymerase chain reaction identification of virulence-associated genes, and phage type 4 (PT4) assessment were performed to characterize Salmonella Enteritidis SE86. The human strains presented less antimicrobial resistance than the poultry strains. Resistance to some substances was related to the isolation source of the strain. Strains of the same clonal group presented different biofilm production abilities. Biofilm formation was independent of the isolation source at all temperatures. Temperature influenced biofilm formation only by the poultry strains. Most of the investigated genes presented a high frequency and a regular distribution, regardless of the isolation source. The spvB, spiA, pagC, sipB, prgH, spaN, sitC, and lpfC genes were associated with the avian strains, whereas iroN was associated with the strains isolated from food products involved in salmonellosis outbreaks. Most strains belonged to PT4. No relationship was found between biofilm production and antimicrobial resistance or between the virulence profile and biofilm production or antimicrobial resistance.", "gemini": "Salmonella Enteritidis vẫn là một trong những nguyên nhân hàng đầu gây ra các bệnh do thực phẩm trên toàn thế giới. Các nghiên cứu trước đây đã chứng minh rằng một nhóm vô tính độc đáo của Salmonella Enteritidis, có tên là SE86, có liên quan đến các đợt bùng phát do thực phẩm ở miền nam Brazil và thường được xác định trong số các chủng phân lập từ gia cầm. Mục đích của nghiên cứu này là xác định ảnh hưởng của nguồn phân lập (các sản phẩm thực phẩm liên quan đến các đợt bùng phát bệnh salmonella và các nguồn gia cầm) đến các đặc điểm về kiểu hình và phân tử của Salmonella Enteritidis SE86. Một xét nghiệm hình thành màng sinh học, xét nghiệm độ nhạy cảm với thuốc kháng khuẩn, phản ứng chuỗi polymerase xác định các gen liên quan đến độc lực và đánh giá kiểu phage 4 (PT4) đã được thực hiện để đặc trưng cho Salmonella Enteritidis SE86. Các chủng ở người ít kháng thuốc hơn các chủng gia cầm. Kháng thuốc đối với một số chất liên quan đến nguồn phân lập của chủng. Các chủng của cùng một nhóm vô tính thể hiện khả năng sản xuất màng sinh học khác nhau. Sự hình thành màng sinh học không phụ thuộc vào nguồn phân lập ở mọi nhiệt độ. Nhiệt độ chỉ ảnh hưởng đến sự hình thành màng sinh học của các chủng gia cầm. Hầu hết các gen được nghiên cứu đều có tần số cao và phân bố đều, bất kể nguồn phân lập. Các gen spvB, spiA, pagC, sipB, prgH, spaN, sitC và lpfC có liên quan đến các chủng gia cầm, trong khi iroN có liên quan đến các chủng phân lập từ các sản phẩm thực phẩm liên quan đến các đợt bùng phát bệnh salmonella. Hầu hết các chủng đều thuộc PT4. Không tìm thấy mối quan hệ nào giữa sản xuất màng sinh học và kháng thuốc hoặc giữa hồ sơ độc lực và sản xuất màng sinh học hoặc kháng thuốc."} +{"idx": 1930044, "en": "AIM To show that an increased correlation between CpGs after selection through an epigenome-wide association studies (EWAS) might translate into biased replication results. METHODS Pairwise correlation coefficients between CpGs selected in two published EWAS, the top hits replication, Bonferroni p-values, Benjamini-Hochberg (BH) false discovery rate (FDR) and directional FDR r-values were calculated in the NINFEA cohort data. Exposures' random permutations were performed to show the empirical p-value distributions. RESULTS The average pairwise correlation coefficients between CpGs were enhanced after selection for the replication (e.g., from 0.12 at genome-wide level to 0.26 among the selected CpGs), affecting the empirical p-value distributions and the usual multiple testing control. CONCLUSION Bonferroni and Benjamini-Hochberg FDR are inappropriate for the EWAS replication phase, and methods that account for the underlying correlation need to be used.", "gemini": "MỤC ĐÍCH Chỉ ra rằng mối tương quan gia tăng giữa các CpG sau khi lựa chọn thông qua các nghiên cứu liên kết trên toàn bộ epigenome (EWAS) có thể dẫn đến kết quả sao chép có sai lệch. PHƯƠNG PHÁP Các hệ số tương quan từng cặp giữa các CpG được chọn trong hai EWAS đã công bố, bản sao chép các kết quả hàng đầu, giá trị p của Bonferroni, tỷ lệ phát hiện sai (FDR) của Benjamini-Hochberg (BH) và các giá trị r của FDR theo hướng đã được tính toán trong dữ liệu nhóm NINFEA. Các hoán vị ngẫu nhiên của các yếu tố phơi nhiễm đã được thực hiện để hiển thị các phân phối giá trị p theo kinh nghiệm. KẾT QUẢ Các hệ số tương quan từng cặp trung bình giữa các CpG đã được tăng cường sau khi lựa chọn để sao chép (ví dụ: từ 0,12 ở mức toàn bộ hệ gen lên 0,26 trong số các CpG được chọn), ảnh hưởng đến các phân phối giá trị p theo kinh nghiệm và kiểm soát thử nghiệm nhiều thông thường. KẾT LUẬN FDR của Bonferroni và Benjamini-Hochberg không phù hợp cho giai đoạn sao chép EWAS và cần sử dụng các phương pháp tính đến mối tương quan cơ bản."} +{"idx": 1930045, "en": "AIM We tested whether genetic variation in miRNA processing genes modified the association of PM2.5 with DNA methylation (DNAm) age. PATIENTS & METHODS We conducted a repeated measures study based on 552 participants from the Normative Aging Study with multiple visits between 2000 and 2011 (n = 940 visits). Address-level 1-year PM2.5 exposures were estimated using the GEOS-chem model. DNAm-age and a panel of 14 SNPs in miRNA processing genes were measured from participant blood samples. RESULTS & CONCLUSION In fully adjusted linear mixed-effects models, having at least one copy of the minor rs4961280 [AGO2] allele was associated with a lower DNAm-age (β = -1.13; 95% CI: -2.26 to -0.002). However, the association of PM2.5 with DNAm-age was significantly (Pinteraction  = 0.01) weaker in homozygous carriers of the major rs4961280 [AGO2] allele (β = 0.38; 95% CI: -0.20 to 0.96) when compared with all other participants (β = 1.58; 95% CI: 0.76 to 2.39). Our results suggest that miRNA processing impacts DNAm-age relationships. Graphical abstract: miRNA processing AGO2 polymorphism (rs4961280) modifies the association of long-term ambient fine particle exposure with blood DNA methylation age [Formula: see text] The graph depicts lines from a fully adjusted linear regression model with fine particle exposure levels ranging from the tenth to the ninetieth percentile, all other continuous variables held constant at their means, and all other categorical variables held at their most frequent level.", "gemini": "MỤC TIÊU Chúng tôi đã kiểm tra xem biến thể di truyền ở các gen xử lý miRNA có làm thay đổi mối liên quan giữa PM2.5 với tuổi methyl hóa DNA (DNAm) hay không. BỆNH NHÂN & PHƯƠNG PHÁP Chúng tôi đã tiến hành một nghiên cứu biện pháp lặp đi lặp lại dựa trên 552 người tham gia từ Nghiên cứu lão hóa chuẩn mực với nhiều lần thăm khám giữa năm 2000 và 2011 (n = 940 lần thăm khám). Tiếp xúc với PM2.5 trong 1 năm ở cấp độ địa chỉ được ước tính bằng mô hình GEOS-chem. DNAm-age và một nhóm 14 SNP trong các gen xử lý miRNA được đo từ các mẫu máu của người tham gia. KẾT QUẢ & KẾT LUẬN Trong các mô hình hiệu ứng hỗn hợp tuyến tính được điều chỉnh đầy đủ, việc có ít nhất một bản sao của alen rs4961280 [AGO2] nhỏ hơn được liên kết với DNAm-age thấp hơn (β = -1,13; 95% CI: -2,26 đến -0,002). Tuy nhiên, mối liên quan giữa PM2.5 với DNAm-age yếu hơn đáng kể (Pinteraction  = 0,01) ở những người mang kiểu gen đồng hợp tử alen rs4961280 [AGO2] chính (β = 0,38; 95% CI: -0,20 đến 0,96) khi so sánh với tất cả những người tham gia khác (β = 1,58; 95% CI: 0,76 đến 2,39). Kết quả của chúng tôi cho thấy quá trình xử lý miRNA ảnh hưởng đến các mối quan hệ DNAm-age. Tóm tắt đồ họa: Đa hình AGO2 xử lý miRNA (rs4961280) làm thay đổi mối liên quan giữa phơi nhiễm hạt mịn xung quanh trong thời gian dài với tuổi methyl hóa DNA trong máu [Công thức: xem văn bản] Đồ thị mô tả các đường từ mô hình hồi quy tuyến tính được điều chỉnh đầy đủ với mức độ phơi nhiễm hạt mịn dao động từ phần trăm thứ mười đến phần trăm thứ chín mươi, tất cả các biến liên tục khác được giữ ở mức trung bình và tất cả các biến phân loại khác được giữ ở mức thường xuyên nhất."} +{"idx": 1930046, "en": "Apple consumption has been demonstrated to be associated with reduced risk of cardiovascular disease, and the beneficial effect is probably due to the polyphenols in apple. Here, we for the first time evaluated and compared the in vivo effects of the polyphenolic extracts of apple peels (PAP) and apple fleshes (PAF) on blood pressure, vascular endothelial function, lipid metabolism and insulin resistance. A high-fat and high-fructose (HFHF) diet was used to cause cardiovascular disorders in mice, with blood pressure, serum ET-1, TC, TG, LDL-C, glucose and insulin levels increased, and serum NO and HDL-C levels decreased. Mice administered with 250 mg/kg of PAP and PAF for 28 days showed lower blood pressure, improved endothelial function, ameliorated lipid homeostasis and decreased insulin resistance compared with HFHF-fed mice. Furthermore, PAP exhibited much more potent cardioprotective effects than PAF in mice. Quantification and phenolic profile analysis showed that PAP contained remarkably higher amount of total phenolics and total flavonoids than PAF, and this may be the reason for the relatively stronger efficacy of PAP. This study demonstrates that apple polyphenols possess potential cardioprotective effects, and suggests that apple, especially apple peel, may be excellent source for exploration of preventive agents against cardiovascular disorders.", "gemini": "Tiêu thụ táo đã được chứng minh là có liên quan đến việc giảm nguy cơ mắc bệnh tim mạch, và tác dụng có lợi này có thể là do các polyphenol trong táo. Ở đây, chúng tôi lần đầu tiên đánh giá và so sánh các tác động in vivo của chiết xuất polyphenol từ vỏ táo (PAP) và thịt táo (PAF) đối với huyết áp, chức năng nội mô mạch máu, chuyển hóa lipid và kháng insulin. Chế độ ăn nhiều chất béo và nhiều fructose (HFHF) được sử dụng để gây ra các rối loạn tim mạch ở chuột, với huyết áp, ET-1 huyết thanh, TC, TG, LDL-C, glucose và nồng độ insulin tăng lên, trong khi nồng độ NO và HDL-C huyết thanh giảm. Chuột được dùng 250 mg/kg PAP và PAF trong 28 ngày cho thấy huyết áp thấp hơn, chức năng nội mô được cải thiện, cân bằng lipid được cải thiện và giảm kháng insulin so với chuột được cho ăn HFHF. Hơn nữa, PAP biểu hiện tác dụng bảo vệ tim mạnh hơn nhiều so với PAF ở chuột. Phân tích định lượng và thành phần phenol cho thấy PAP chứa lượng tổng phenol và tổng flavonoid cao hơn đáng kể so với PAF, và đây có thể là lý do khiến PAP có hiệu quả tương đối mạnh hơn. Nghiên cứu này chứng minh rằng polyphenol táo có tác dụng bảo vệ tim tiềm tàng và cho thấy rằng táo, đặc biệt là vỏ táo, có thể là nguồn tuyệt vời để khám phá các tác nhân phòng ngừa chống lại các rối loạn tim mạch."} +{"idx": 1930047, "en": "Studies under real life conditions become more and more relevant in chronobiological and chronomedical research. The present study aims to analyze one of the most prominent biological rhythms: the core body temperature (CBT) rhythm in the real world outside the laboratory. CBT was recorded continuously in 37 healthy women (age between 21 and 44 years, median 29 years) with a newly developed intravaginal temperature sensor for up to 102 days. Sleep logs were available from 23 participants. To quantify the daily dynamics of each individual CBT-curve, novel measurement parameters are introduced which permit the quantification of the phase and shape of the CBT rhythms as well as their relation to the sleep-wake cycle. In addition to the classical phase markers (i.e. nadir and peak), the daily curves were segmented into quartiles by introducing the t25/t50/t75-values which can be used as phase and shape markers. At variance to previous studies, a conspicuous day-to-day variation was shown not only for the time point of the peak, but also for the time point of the nadir. However, the t-values, particularly the t75-value were relatively closely locked to external time and thus represent more reliable phase markers than the nadir. The (variable) time point of the nadir determined the period length, phase and shape of the subsequent CBT cycle. If a nadir occurred close to the wake-up time, the following cycle was considerably shorter than 24 hours, while a nadir distant from the wake-up time was followed by a longer cycle. Thus, the period lengths of the daily CBT cycles of each individual were characterized by an \"expand/contract\" rhythm. The analyses of the novel phase markers (t25/t50/t75) of the CBT curves allowed to identify \"early\" and \"late\" participants who may differ in their phase-response curves with regard to the entraining effect of light. In addition, the novel phase markers mirrored the different social entrainment conditions on weekends and workdays.", "gemini": "Các nghiên cứu trong điều kiện thực tế ngày càng trở nên phù hợp hơn trong nghiên cứu sinh học thời gian và y học thời gian. Nghiên cứu hiện tại nhằm phân tích một trong những nhịp sinh học nổi bật nhất: nhịp nhiệt độ cơ thể cốt lõi (CBT) trong thế giới thực bên ngoài phòng thí nghiệm. CBT được ghi liên tục ở 37 phụ nữ khỏe mạnh (tuổi từ 21 đến 44, trung vị 29 tuổi) bằng cảm biến nhiệt độ âm đạo mới được phát triển trong tối đa 102 ngày. Nhật ký giấc ngủ có sẵn từ 23 người tham gia. Để định lượng động lực hàng ngày của từng đường cong CBT riêng lẻ, các thông số đo lường mới được đưa ra cho phép định lượng pha và hình dạng của nhịp CBT cũng như mối quan hệ của chúng với chu kỳ ngủ-thức. Ngoài các điểm đánh dấu pha cổ điển (tức là điểm thấp nhất và điểm cao nhất), các đường cong hàng ngày được chia thành bốn phần bằng cách đưa ra các giá trị t25/t50/t75 có thể được sử dụng làm điểm đánh dấu pha và hình dạng. Trái ngược với các nghiên cứu trước đây, sự thay đổi đáng kể từng ngày đã được chỉ ra không chỉ đối với thời điểm đạt đỉnh mà còn đối với thời điểm đạt điểm thấp nhất. Tuy nhiên, các giá trị t, đặc biệt là giá trị t75 tương đối gần với thời gian bên ngoài và do đó thể hiện các điểm đánh dấu pha đáng tin cậy hơn so với điểm thấp nhất. Thời điểm (biến đổi) của điểm thấp nhất xác định độ dài chu kỳ, pha và hình dạng của chu kỳ CBT tiếp theo. Nếu điểm thấp nhất xảy ra gần thời điểm thức dậy, chu kỳ tiếp theo sẽ ngắn hơn đáng kể so với 24 giờ, trong khi điểm thấp nhất cách xa thời điểm thức dậy thì tiếp theo là một chu kỳ dài hơn. Do đó, độ dài chu kỳ của các chu kỳ CBT hàng ngày của mỗi cá nhân được đặc trưng bởi nhịp \"mở rộng/thu hẹp\". Các phân tích về các điểm đánh dấu pha mới (t25/t50/t75) của đường cong CBT cho phép xác định những người tham gia \"sớm\" và \"muộn\" có thể khác nhau về đường cong đáp ứng pha của họ liên quan đến tác dụng lôi kéo của ánh sáng. Ngoài ra, các điểm đánh dấu pha mới phản ánh các điều kiện lôi kéo xã hội khác nhau vào cuối tuần và ngày làm việc."} +{"idx": 1930048, "en": "OBJECTIVE To evaluate and compare two treatment protocols to correct anterior dental crossbite in the mixed dentition. MATERIALS AND METHODS Thirty children, 8-10 years of age, participated. Individuals were divided into two groups. Group 1 consisted of 15 children treated with an upper removable appliance with finger springs; group 2, 15 children treated by bonding resin-reinforced glass ionomer cement bite pads on the lower first molars. The 30 participants were evaluated before treatment (T1) and 12 months after treatment began (T2). The variables evaluated included overjet, perimeter of the maxillary arch, intercanine distances in the maxilla and mandible, SNA, SNB, ANB, and U1.NA. Data analysis included descriptive statistics, paired t-test and Student's t-test. Effect sizes and confidence intervals were also calculated. RESULTS Group 1 showed a significant increase in overjet ( P < .001), intercanine distance in the maxilla ( P = .006), intercanine distance in the mandible ( P = .031), and U1.NA ( P = .002). Group 2 showed a significant increase in overjet ( P = .008), intercanine distance in the mandible ( P = .005), and U1.NA ( P < .001). For all the evaluated variables, no statistically significant differences were observed between the two groups. CONCLUSIONS No significant differences were observed between the two protocols: use of a removable maxillary biteplate with finger springs and bonding of resin-reinforced glass ionomer cement bite pads on the lower first molars, for the correction of anterior crossbite in the mixed dentition.", "gemini": "MỤC TIÊU Đánh giá và so sánh hai phác đồ điều trị để chỉnh sửa tình trạng cắn chéo răng trước trong quá trình mọc răng hỗn hợp. VẬT LIỆU VÀ PHƯƠNG PHÁP Ba mươi trẻ em từ 8 đến 10 tuổi đã tham gia. Những cá nhân được chia thành hai nhóm. Nhóm 1 gồm 15 trẻ được điều trị bằng khí cụ tháo lắp hàm trên có lò xo ngón tay; nhóm 2, 15 trẻ được điều trị bằng cách liên kết miếng đệm cắn xi măng ion kim loại được gia cố bằng nhựa trên răng hàm đầu tiên hàm dưới. 30 người tham gia được đánh giá trước khi điều trị (T1) và 12 tháng sau khi bắt đầu điều trị (T2). Các biến được đánh giá bao gồm khoảng cách cắn chìa, chu vi cung hàm trên, khoảng cách giữa răng nanh ở hàm trên và hàm dưới, SNA, SNB, ANB và U1.NA. Phân tích dữ liệu bao gồm thống kê mô tả, kiểm định t ghép đôi và kiểm định t của Student. Cỡ hiệu ứng và khoảng tin cậy cũng được tính toán. KẾT QUẢ Nhóm 1 cho thấy sự gia tăng đáng kể về khoảng cách cắn chìa (P < 0,001), khoảng cách giữa răng nanh ở hàm trên (P = 0,006), khoảng cách giữa răng nanh ở hàm dưới (P = 0,031) và U1.NA (P = 0,002). Nhóm 2 cho thấy sự gia tăng đáng kể về khoảng cách cắn chìa (P = 0,008), khoảng cách giữa răng nanh ở hàm dưới (P = 0,005) và U1.NA (P < 0,001). Đối với tất cả các biến được đánh giá, không có sự khác biệt có ý nghĩa thống kê nào được quan sát giữa hai nhóm. KẾT LUẬN Không có sự khác biệt đáng kể nào được quan sát giữa hai phác đồ: sử dụng máng cắn tháo lắp hàm trên có lò xo ngón tay và liên kết miếng đệm cắn xi măng ion kim loại được gia cố bằng nhựa trên răng hàm đầu tiên hàm dưới để chỉnh sửa tình trạng cắn chéo răng trước trong quá trình mọc răng hỗn hợp."} +{"idx": 1930049, "en": "Current advances in immunology have led to the identification of a population of novel innate immune T cells, called mucosa-associated invariant T (MAIT) cells. The cells in humans express an invariant TCRα chain (Vα7.2-Jα33) paired with a limited subset of TCRβ chains (Vβ2, 13 and 22), are restricted by the MHC class I (MH1)-related (MR)-1, and recognize molecules that are produced in the bacterial riboflavin (vitamin B2) biosynthetic pathway. They are present in the circulation, liver and at various mucosal sites (i.e. intestine, lungs and female reproductive tract, etc.). They kill host cells infected with bacteria and yeast, and secrete soluble mediators such as TNF-α, IFN-γ, IL-17, etc. The cells regulate immune responses and inflammation associated with a wide spectrum of acute and chronic diseases in humans. Since their discovery in 1993, significant advances have been made in understanding biology of MAIT cells and the potential role of these cells in the pathogenesis of autoimmune, inflammatory and infectious diseases as well as cancer in humans. The purpose of this review is to provide a current state of our knowledge about MAIT cell biology and delineate their role in autoimmune and inflammatory diseases (sterile or caused by infectious agents) and cancer in humans. A better understanding of the role of MAIT cells in human diseases may lead to novel ways of immunotherapies.", "gemini": "Những tiến bộ hiện tại trong miễn dịch học đã dẫn đến việc xác định một quần thể tế bào T miễn dịch bẩm sinh mới, được gọi là tế bào T bất biến liên quan đến niêm mạc (MAIT). Các tế bào ở người biểu hiện một chuỗi TCRα bất biến (Vα7.2-Jα33) ghép nối với một tập hợp con giới hạn các chuỗi TCRβ (Vβ2, 13 và 22), bị hạn chế bởi MHC lớp I (MH1) liên quan (MR) -1 và nhận ra các phân tử được sản xuất trong con đường sinh tổng hợp riboflavin (vitamin B2) của vi khuẩn. Chúng có mặt trong hệ tuần hoàn, gan và tại các vị trí niêm mạc khác nhau (ví dụ: ruột, phổi và đường sinh sản của phụ nữ, v.v.). Chúng tiêu diệt các tế bào chủ bị nhiễm vi khuẩn và nấm men, và tiết ra các chất trung gian hòa tan như TNF-α, IFN-γ, IL-17, v.v. Các tế bào điều chỉnh phản ứng miễn dịch và tình trạng viêm liên quan đến nhiều loại bệnh cấp tính và mãn tính ở người. Kể từ khi được phát hiện vào năm 1993, những tiến bộ đáng kể đã đạt được trong việc hiểu sinh học của tế bào MAIT và vai trò tiềm tàng của các tế bào này trong sinh bệnh của bệnh tự miễn, viêm và truyền nhiễm cũng như ung thư ở người. Mục đích của bài tổng quan này là cung cấp tình trạng kiến thức hiện tại của chúng tôi về sinh học tế bào MAIT và mô tả vai trò của chúng trong các bệnh tự miễn và viêm (vô trùng hoặc do tác nhân truyền nhiễm gây ra) và ung thư ở người. Việc hiểu rõ hơn về vai trò của tế bào MAIT trong các bệnh của con người có thể dẫn đến những cách thức mới về liệu pháp miễn dịch."} +{"idx": 1930050, "en": "OBJECTIVE Considering the role of anti-Müllerian hormone (AMH) in female fertility and its high levels in women with polycystic ovary syndrome (PCOS), the longer reproductive span of these women is in doubt. In the present study, we aimed to improve earlier predictions using a non-linear model to substantiate the question as to whether PCOS women reach menopause later. METHODS In total, 1162 women aged 20-50 years, comprising 378 PCOS cases and 784 eumenorrheic non-hirsute women, met the eligibility criteria. A scatterplot matrix was drawn to detect the association between age and AMH; this association was explored using a fractional polynomial regression model. Model assumptions were checked by examining the distribution of the residuals and plotting the standardized residuals against the functional form of AMH. RESULTS The serum concentration of AMH among PCOS participants was significantly higher than in the controls (5.4 ng/ml (IQR 2.8-9.1 ng/ml) vs. 1.4 ng/ml (IQR 0.6-2.7 ng/ml), p < 0.001). The estimated mean age at menopause was 51.4 (95% CI 45-59) years and 49.7 (95% CI 45-55) years in PCOS cases and healthy controls, respectively. CONCLUSIONS These findings provide the insight that, as reflected through significantly higher average levels of AMH in PCOS women, their predicted reproductive lifespan could be 2 years longer than their normo-ovulatory counterparts.", "gemini": "MỤC TIÊU Xét đến vai trò của hormone chống Muller (AMH) trong khả năng sinh sản của phụ nữ và nồng độ cao của nó ở những phụ nữ mắc hội chứng buồng trứng đa nang (PCOS), thời gian sinh sản kéo dài hơn của những phụ nữ này đang bị nghi ngờ. Trong nghiên cứu hiện tại, chúng tôi nhằm mục đích cải thiện các dự đoán trước đó bằng cách sử dụng một mô hình phi tuyến tính để chứng minh cho câu hỏi liệu phụ nữ PCOS có mãn kinh muộn hơn hay không. PHƯƠNG PHÁP Tổng cộng, 1162 phụ nữ trong độ tuổi từ 20-50, bao gồm 378 trường hợp PCOS và 784 phụ nữ kinh nguyệt đều không rậm lông, đáp ứng các tiêu chuẩn đủ điều kiện. Một ma trận biểu đồ phân tán đã được vẽ để phát hiện mối liên quan giữa tuổi và AMH; mối liên quan này đã được khám phá bằng cách sử dụng mô hình hồi quy đa thức phân số. Các giả định của mô hình đã được kiểm tra bằng cách kiểm tra phân phối của các giá trị còn lại và vẽ biểu đồ các giá trị còn lại đã chuẩn hóa so với dạng chức năng của AMH. KẾT QUẢ Nồng độ AMH trong huyết thanh ở những người tham gia PCOS cao hơn đáng kể so với nhóm đối chứng (5,4 ng/ml (IQR 2,8-9,1 ng/ml) so với 1,4 ng/ml (IQR 0,6-2,7 ng/ml), p < 0,001). Tuổi trung bình ước tính khi mãn kinh là 51,4 (95% CI 45-59) năm và 49,7 (95% CI 45-55) năm ở những trường hợp PCOS và nhóm đối chứng khỏe mạnh. KẾT LUẬN Những phát hiện này cung cấp cái nhìn sâu sắc rằng, như được phản ánh thông qua mức AMH trung bình cao hơn đáng kể ở phụ nữ PCOS, tuổi thọ sinh sản dự đoán của họ có thể dài hơn 2 năm so với những người có rụng trứng bình thường."} +{"idx": 1930051, "en": "Electronic clinical summaries are innovations supported by the Electronic Health Record Incentive Program, known as \"Meaningful Use\" (MU). The MU clinical summary documents the shared understanding of the plan of care for patients and assists families in managing asthma-related health care. The purpose of this analysis was to identify the communicative value of the summaries to patients and families. Readability measurements, content analysis, and descriptive statistics were employed in a review of twenty clinical summaries and compared with provider encounter notes. The average age of the patients from whom we collected clinical summaries was six years old. The average reading level of the summaries was ninth grade. Neither summaries nor health education contained visual images. There was a total of nine different asthma diagnoses. A full list of diagnoses was present in 45% of summaries. The average medications per patient was 5.75, and there were multiple medication changes noted (dosage adjustment, add, discontinued). Allergies, vital signs, and smoking status were reliably reported (99-100%). Provider orders present included medication, follow up, and return to the clinic instructions. The plan of care was replicated on 45% of summaries. There was variable reporting of various asthma guidelines. Opportunities to improve the clinical summary include using plain language to promote readability, action, understanding, and health literacy, training providers to standardize their documentation and include asthma action plans, and configuring EHR settings to ensure diagnoses and plan of care is carried over from provider notes to the summary.", "gemini": "Các bản tóm tắt lâm sàng điện tử là những sáng kiến được Chương trình khuyến khích hồ sơ sức khỏe điện tử, được gọi là \"Sử dụng có ý nghĩa\" (MU) hỗ trợ. Bản tóm tắt lâm sàng MU ghi lại sự hiểu biết chung về kế hoạch chăm sóc cho bệnh nhân và hỗ trợ các gia đình trong việc quản lý chăm sóc sức khỏe liên quan đến hen suyễn. Mục đích của phân tích này là xác định giá trị giao tiếp của các bản tóm tắt đối với bệnh nhân và gia đình. Các phép đo khả năng đọc, phân tích nội dung và thống kê mô tả đã được sử dụng trong quá trình xem xét 20 bản tóm tắt lâm sàng và so sánh với các ghi chú tiếp xúc của nhà cung cấp. Độ tuổi trung bình của những bệnh nhân mà chúng tôi thu thập bản tóm tắt lâm sàng là sáu tuổi. Trình độ đọc trung bình của các bản tóm tắt là lớp chín. Không có bản tóm tắt nào cũng như giáo dục sức khỏe nào có hình ảnh trực quan. Có tổng cộng chín chẩn đoán hen suyễn khác nhau. Danh sách đầy đủ các chẩn đoán có trong 45% bản tóm tắt. Trung bình thuốc trên mỗi bệnh nhân là 5,75 và có nhiều thay đổi về thuốc được ghi nhận (điều chỉnh liều lượng, thêm, ngừng). Dị ứng, các dấu hiệu sinh tồn và tình trạng hút thuốc được báo cáo đáng tin cậy (99-100%). Các đơn đặt hàng của nhà cung cấp hiện có bao gồm thuốc, theo dõi và quay lại hướng dẫn phòng khám. Kế hoạch chăm sóc được sao chép trên 45% bản tóm tắt. Có báo cáo khác nhau về các hướng dẫn khác nhau về hen suyễn. Các cơ hội để cải thiện bản tóm tắt lâm sàng bao gồm sử dụng ngôn ngữ đơn giản để thúc đẩy khả năng đọc, hành động, hiểu biết và hiểu biết về sức khỏe, đào tạo các nhà cung cấp để chuẩn hóa tài liệu của họ và bao gồm các kế hoạch hành động về hen suyễn và định cấu hình cài đặt EHR để đảm bảo chẩn đoán và kế hoạch chăm sóc được chuyển từ ghi chú của nhà cung cấp sang bản tóm tắt."} +{"idx": 1930052, "en": "BACKGROUND The menopausal transition may have significant consequences for respiratory health, risk of chronic respiratory disease and management strategies. OBJECTIVE To systematically summarize the literature regarding the impact of menopause status on respiratory health outcomes. METHODS PubMed was searched systematically to identify population-based studies investigating the associations between menopause status and respiratory outcomes including asthma, chronic obstructive pulmonary disease (COPD), respiratory symptoms and lung function. RESULTS Ten publications were identified for full review. Evidence on menopause and asthma was conflicting, while studies on COPD were scarce. The findings generally support an association between menopause and clinically significant reductions in lung function in a non-obstructive pattern. However, the effects of menopause are clouded by aging, menopausal hormone therapy use, and increased risk of metabolic syndrome during this period. CONCLUSIONS As the global burden associated with respiratory conditions continues to rise, the need to understand the associations between menopause and respiratory health is essential to identify potentially modifiable risk factors for respiratory disease in adult women. More studies are needed to clarify the impact of menopause on obstructive lung disease.", "gemini": "HOÀN CẢNH Quá trình mãn kinh có thể có những hậu quả đáng kể đối với sức khỏe hô hấp, nguy cơ mắc bệnh hô hấp mãn tính và các chiến lược quản lý. MỤC TIÊU Tóm tắt có hệ thống các tài liệu về tác động của tình trạng mãn kinh đối với kết quả sức khỏe hô hấp. PHƯƠNG PHÁP PubMed đã được tìm kiếm một cách có hệ thống để xác định các nghiên cứu dựa trên dân số để điều tra mối liên quan giữa tình trạng mãn kinh và các kết quả hô hấp bao gồm hen suyễn, bệnh phổi tắc nghẽn mãn tính (COPD), các triệu chứng về hô hấp và chức năng phổi. KẾT QUẢ Mười ấn phẩm đã được xác định để xem xét đầy đủ. Bằng chứng về mãn kinh và hen suyễn là mâu thuẫn, trong khi các nghiên cứu về COPD còn rất ít. Các phát hiện nói chung ủng hộ mối liên quan giữa mãn kinh và sự giảm chức năng phổi đáng kể về mặt lâm sàng theo kiểu không tắc nghẽn. Tuy nhiên, các tác động của mãn kinh bị lu mờ bởi quá trình lão hóa, sử dụng liệu pháp hormone mãn kinh và tăng nguy cơ mắc hội chứng chuyển hóa trong giai đoạn này. KẾT LUẬN Khi gánh nặng toàn cầu liên quan đến các tình trạng hô hấp tiếp tục gia tăng, việc cần hiểu các mối liên quan giữa mãn kinh và sức khỏe hô hấp là rất cần thiết để xác định các yếu tố nguy cơ có thể thay đổi được đối với bệnh hô hấp ở phụ nữ trưởng thành. Cần nhiều nghiên cứu hơn để làm rõ tác động của mãn kinh đối với bệnh phổi tắc nghẽn."} +{"idx": 1930053, "en": "AIM To investigate the role of maternal gestational weight gain (GWG) and prepregnancy BMI on programming offspring DNA methylation. METHODS Among 589 adult (age = 32) women participants of the Jerusalem Perinatal Study, we quantified DNA methylation in five candidate genes. We used inverse probability-weighting and parametric g-formula to estimate direct effects of maternal prepregnancy BMI and GWG on methylation. RESULTS Higher maternal GWG, but not prepregnancy BMI, was inversely related to offspring ABCA1 methylation (β = -1.1% per quartile; 95% CI: -2.0, -0.3) after accounting for ancestry, parental and offspring exposures. Total and controlled direct effects were nearly identical suggesting included offspring exposures did not mediate this relationship. Results were robust to sensitivity analyses for missing data and model specification. CONCLUSION We find some support for epigenetic programming and highlight strengths and limitations of these methods relative to other prevailing approaches.", "gemini": "MỤC ĐÍCH Để điều tra vai trò của tăng cân khi mang thai của người mẹ (GWG) và BMI trước khi mang thai đối với quá trình lập trình metyl hóa DNA của con. PHƯƠNG PHÁP Trong số 589 phụ nữ tham gia Nghiên cứu tiền sản Jerusalem (tuổi = 32), chúng tôi định lượng metyl hóa DNA ở năm gen ứng viên. Chúng tôi đã sử dụng phép cân trọng nghịch đảo xác suất và công thức g tham số để ước tính các tác động trực tiếp của BMI trước khi mang thai của người mẹ và GWG đối với quá trình metyl hóa. KẾT QUẢ GWG của người mẹ cao hơn, nhưng không phải BMI trước khi mang thai, có liên quan nghịch với metyl hóa ABCA1 của con (β = -1,1% trên mỗi tứ phân vị; 95% CI: -2,0, -0,3) sau khi tính đến nguồn gốc, tiếp xúc của cha mẹ và con. Các tác động trực tiếp tổng thể và được kiểm soát gần như giống hệt nhau, cho thấy các tiếp xúc với con được bao gồm không ảnh hưởng đến mối quan hệ này. Kết quả vẫn mạnh mẽ đối với các phân tích độ nhạy đối với dữ liệu bị thiếu và thông số mô hình. KẾT LUẬN Chúng tôi tìm thấy một số hỗ trợ cho lập trình biểu sinh và làm nổi bật những điểm mạnh và hạn chế của các phương pháp này so với các phương pháp tiếp cận phổ biến khác."} +{"idx": 1930054, "en": "OBJECTIVES The study assessed the psychosocial impact of the bihormonal bionic pancreas (BP) on adults in a real-world outpatient setting. RESEARCH DESIGN AND METHODS Thirty-nine adults with type 1 diabetes at four study centers across the U.S. participated in a two-arm, random-order, crossover design study: 11 days with the BP and 11 days with their usual care (UC). Psychosocial questionnaires were administered before the first study arm, at the end of the first study arm, and at the end of the second study arm. RESULTS The mean age of participants was 33 years; mean diabetes duration was 17 years; and 84% were non-Hispanic Caucasian. Significantly greater improvements in psychosocial outcomes were found following the use of BP versus UC; these included reductions in diabetes-related distress (P < 0.001) with the greatest drops in distress related to hypoglycemia and to eating constraints, and greater treatment satisfaction (P < 0.05). The majority of BP users described their experience as positive with a reduction in worrying about low (72%) and high (69%) blood sugars. The majority reported trusting the device (69%) and feeling less burdened by the BP than their usual method of diabetes care (64%). Concerns about the burden of the BP were also expressed, with >75% of users highlighting the burden of carrying around the equipment and the need to change glucagon daily, and more than half of the participants reporting concerns about wearability, discomfort, the time it took to correct out-of-range numbers, and \"glitches\" in the system. CONCLUSIONS Overall, participants report substantial psychosocial benefits accruing from the BP relative to their usual method of diabetes care. However, participants also reported a number of burdens associated with the system. Future versions of the BP device should be designed with the goal of addressing these concerns, and studies with larger, more diverse samples, and with more technology-naive participants are needed.", "gemini": "MỤC TIÊU Nghiên cứu đánh giá tác động tâm lý xã hội của tụy nhân tạo sinh học (BP) hai hormon ở người lớn trong môi trường ngoại trú thực tế. THIẾT KẾ NGHIÊN CỨU VÀ PHƯƠNG PHÁP Ba mươi chín người lớn mắc bệnh tiểu đường týp 1 tại bốn trung tâm nghiên cứu trên khắp Hoa Kỳ đã tham gia vào một nghiên cứu thiết kế chéo, thứ tự ngẫu nhiên, hai cánh tay: 11 ngày dùng BP và 11 ngày dùng chế độ chăm sóc thông thường (UC) của họ. Các bảng câu hỏi tâm lý xã hội đã được thực hiện trước cánh tay nghiên cứu đầu tiên, vào cuối cánh tay nghiên cứu đầu tiên và vào cuối cánh tay nghiên cứu thứ hai. KẾT QUẢ Tuổi trung bình của những người tham gia là 33; thời gian mắc bệnh tiểu đường trung bình là 17 năm; và 84% là người da trắng không phải gốc Tây Ban Nha. Những cải thiện đáng kể về kết quả tâm lý xã hội đã được tìm thấy sau khi sử dụng BP so với UC; những cải thiện này bao gồm giảm đau khổ liên quan đến bệnh tiểu đường (P < 0,001) với mức giảm đau khổ lớn nhất liên quan đến hạ đường huyết và hạn chế ăn uống, và sự hài lòng về điều trị cao hơn (P < 0,05). Phần lớn người dùng BP mô tả trải nghiệm của họ là tích cực với việc giảm lo lắng về lượng đường trong máu thấp (72%) và cao (69%). Phần lớn báo cáo tin tưởng vào thiết bị (69%) và cảm thấy bớt gánh nặng hơn khi sử dụng BP so với phương pháp chăm sóc bệnh tiểu đường thông thường của họ (64%). Những lo ngại về gánh nặng của BP cũng được nêu ra, với hơn 75% người dùng nêu bật gánh nặng khi mang theo thiết bị và nhu cầu phải thay đổi glucagon hàng ngày, và hơn một nửa số người tham gia báo cáo những lo ngại về khả năng đeo, khó chịu, thời gian cần để sửa những con số ngoài phạm vi và \"trục trặc\" trong hệ thống. KẾT LUẬN Nhìn chung, những người tham gia báo cáo những lợi ích tâm lý xã hội đáng kể phát sinh từ BP so với phương pháp chăm sóc bệnh tiểu đường thông thường của họ. Tuy nhiên, những người tham gia cũng báo cáo một số gánh nặng liên quan đến hệ thống. Các phiên bản thiết bị BP trong tương lai cần được thiết kế với mục tiêu giải quyết những mối quan tâm này và cần có các nghiên cứu với các mẫu lớn hơn, đa dạng hơn và với những người tham gia ít hiểu biết về công nghệ hơn."} +{"idx": 1930055, "en": "Observed enrichments of N (and the δ15N of this N) in volcanic glasses altered on Earth's modern and ancient seafloor are relevant in considerations of modern global N subduction fluxes and ancient life on Earth, and similarly altered glasses on Mars and other extraterrestrial bodies could serve as valuable tracers of biogeochemical processes. Palagonitized glasses and whole-rock samples of volcanic rocks on the modern seafloor (ODP Site 1256D) contain 3-18 ppm N with δ15Nair values of up to +4.5‰. Variably altered glasses from Mesozoic ophiolites (Troodos, Cyprus; Stonyford volcanics, USA) contain 2-53 ppm N with δ15N of -6.3 to +7‰. All of the more altered glasses have N concentrations higher than those of fresh volcanic glass (for MORB, <2 ppm N), reflecting significant N enrichment, and most of the altered glasses have δ15N considerably higher than that of their unaltered glass equivalents (for MORB, -5 ± 2‰). Circulation of hydrothermal fluids, in part induced by nearby spreading-center magmatism, could have leached NH4+ from sediments then fixed this NH4+ in altering volcanic glasses. Glasses from each site contain possible textural evidence for microbial activity in the form of microtubules, but any role of microbes in producing the N enrichments and elevated δ15N remains uncertain. Petrographic analysis, and imaging and chemical analyses by scanning electron microscopy and scanning transmission electron microscopy, indicate the presence of phyllosilicates (smectite, illite) in both the palagonitized cracks and the microtubules. These phyllosilicates (particularly illite), and possibly also zeolites, are the likely hosts for N in these glasses. Key Words: Nitrogen-Nitrogen isotope-Palagonite-Volcanic glass-Mars. Astrobiology 18, 330-342.", "gemini": "Sự làm giàu quan sát được của N (và δ15N của N này) trong các loại thủy tinh núi lửa bị biến đổi trên đáy biển hiện đại và cổ đại của Trái đất có liên quan đến các cân nhắc về dòng chìm N toàn cầu hiện đại và sự sống cổ đại trên Trái đất, và các loại thủy tinh bị biến đổi tương tự trên Sao Hỏa và các thiên thể khác có thể đóng vai trò là chất chỉ thị có giá trị của các quá trình địa hóa sinh. Các loại thủy tinh palagonit hóa và các mẫu đá toàn phần của đá núi lửa trên đáy biển hiện đại (Địa điểm 1256D của ODP) chứa 3-18 ppm N với các giá trị δ15Nair lên đến + 4,5‰. Các loại thủy tinh bị biến đổi khác nhau từ ophiolit Mesozoi (Troodos, Síp; núi lửa Stonyford, Hoa Kỳ) chứa 2-53 ppm N với δ15N từ -6,3 đến + 7‰. Tất cả các loại thủy tinh bị biến đổi nhiều hơn đều có nồng độ N cao hơn so với thủy tinh núi lửa tươi (đối với MORB, <2 ppm N), phản ánh sự làm giàu N đáng kể và hầu hết các loại thủy tinh bị biến đổi đều có δ15N cao hơn đáng kể so với các loại thủy tinh tương đương không bị biến đổi của chúng (đối với MORB, -5 ± 2‰). Sự lưu thông của chất lỏng thủy nhiệt, một phần do sự hình thành khối u lan tỏa gần đó, có thể đã thẩm thấu NH4 + từ trầm tích sau đó cố định NH4 + này trong quá trình biến đổi thủy tinh núi lửa. Các loại thủy tinh từ mỗi địa điểm đều chứa bằng chứng kết cấu có thể có về hoạt động của vi sinh vật dưới dạng vi ống, nhưng bất kỳ vai trò nào của vi sinh vật trong việc tạo ra sự làm giàu N và δ15N cao vẫn chưa chắc chắn. Phân tích khoáng vật học, hình ảnh và phân tích hóa học bằng kính hiển vi điện tử quét và kính hiển vi điện tử truyền qua quét cho thấy sự hiện diện của phyllosilicat (smectite, illite) trong cả các vết nứt palagonit hóa và vi ống. Các phyllosilicat này (đặc biệt là illite) và có thể là cả zeolit, có khả năng là vật chủ của N trong các loại thủy tinh này. Từ khóa: Đồng vị nitơ-nitơ-Palagonite-Thủy tinh núi lửa-Sao Hỏa. Sinh học vũ trụ 18, 330-342."} +{"idx": 1930056, "en": "Our aim is to investigate the prevalence and risk factors associated with hypertension among the Chinese Qiang population. From September 2012 to March 2013, a cross-sectional study was conducted in urban and rural communities of the Qiang population using multistage cluster sampling. A total of 2676 people aged above 20 years were enrolled in the analysis. Standardized mercury sphygmomanometer was used to measure the blood pressure twice after a 10-minute seated rest, and the average blood pressure was obtained. The hypertension prevalence among the population aged above 20 years was 13.9%, and age-standardized prevalence was 12.3%. Male and female prevalence of hypertension, as well as the prevalence in urban and rural areas, all increased with age. There were no significant differences between males and females and between urban and rural residents. Among hypertensive patients, 44.2% were aware of their hypertension, 38.0% were undergoing antihypertensive treatment, but only 10.5% achieved blood pressure control. Multivariate logistic regression analysis showed that the risk factors of hypertension included age, low income, overweight and obesity, family history of hypertension. The prevalence of hypertension in Chinese Qiang adults is significantly lower than the national level. Awareness, treatment, and control rates of hypertension were low in the Qiang population. Thus, hypertension-related health knowledge should be more aggressively delivered to improve public awareness and the capacity of community health services should be strengthened.", "gemini": "Mục đích của chúng tôi là điều tra tỷ lệ mắc và các yếu tố nguy cơ liên quan đến tăng huyết áp trong quần thể người Qiang Trung Quốc. Từ tháng 9 năm 2012 đến tháng 3 năm 2013, một nghiên cứu cắt ngang đã được tiến hành tại các cộng đồng thành thị và nông thôn của người Qiang bằng phương pháp lấy mẫu theo chùm nhiều giai đoạn. Tổng cộng có 2676 người từ 20 tuổi trở lên được đưa vào phân tích. Máy đo huyết áp thủy ngân chuẩn đã được sử dụng để đo huyết áp hai lần sau khi nghỉ ngơi ngồi 10 phút và thu được huyết áp trung bình. Tỷ lệ mắc tăng huyết áp trong dân số trên 20 tuổi là 13,9% và tỷ lệ mắc chuẩn hóa theo tuổi là 12,3%. Tỷ lệ mắc tăng huyết áp ở nam và nữ, cũng như tỷ lệ mắc ở khu vực thành thị và nông thôn đều tăng theo tuổi. Không có sự khác biệt đáng kể giữa nam và nữ và giữa cư dân thành thị và nông thôn. Trong số những bệnh nhân tăng huyết áp, 44,2% biết mình bị tăng huyết áp, 38,0% đang được điều trị hạ huyết áp, nhưng chỉ có 10,5% đạt được mục tiêu kiểm soát huyết áp. Phân tích hồi quy logistic đa biến cho thấy các yếu tố nguy cơ của tăng huyết áp bao gồm tuổi tác, thu nhập thấp, thừa cân và béo phì, tiền sử tăng huyết áp trong gia đình. Tỷ lệ mắc tăng huyết áp ở người lớn người Qiang Trung Quốc thấp hơn đáng kể so với mức quốc gia. Tỷ lệ nhận biết, điều trị và kiểm soát tăng huyết áp thấp ở người Qiang. Do đó, cần truyền đạt kiến thức về sức khỏe liên quan đến tăng huyết áp mạnh mẽ hơn để nâng cao nhận thức của cộng đồng và tăng cường năng lực của các dịch vụ y tế cộng đồng."} +{"idx": 1930057, "en": "The standard of care for monitoring renal transplant patients include transplant biopsy and serum creatinine measurements. These are invasive procedures and are late and nonspecific markers of injury. Proteomics and proteins can provide biomarkers for monitoring pathology and become a useful tool in detection and treatment of conditions that occur after transplant. Areas covered: A survey of 273 studies in the biomarker detection field which included proteomics relating to renal pathology and normal controls was done. Analysis of this data showed pathways and biomarkers specific to different pathologies such as: AR, CR, IRI, TI, VR, DGF, AABMR, ACMR, immunosuppressant toxicity, and infection. It also revealed biomarkers proposed for better detection of these pathologies and the strength (sensitivity and specificity) of such biomarkers. Finally, the field of proteomics in renal transplant in terms of its methodologies, current challenges in clinical translation, and possible solutions are also discussed. Expert commentary: An analysis of biomarkers of acute and chronic rejection revealed acute rejection may be a more inflammatory process. A comparison of proteomic and protein based (n = 183), transcriptomic (n = 1), and genomic (n = 4) studies revealed that proteomic and protein based approaches may offer a clearer picture of inflammation in acute rejection.", "gemini": "Tiêu chuẩn chăm sóc để theo dõi bệnh nhân ghép thận bao gồm sinh thiết ghép và đo creatinine huyết thanh. Đây là những thủ thuật xâm lấn và là các dấu hiệu tổn thương muộn và không đặc hiệu. Proteomics và protein có thể cung cấp các dấu ấn sinh học để theo dõi bệnh lý và trở thành một công cụ hữu ích trong việc phát hiện và điều trị các tình trạng xảy ra sau khi ghép. Các lĩnh vực được đề cập: Một cuộc khảo sát về 273 nghiên cứu trong lĩnh vực phát hiện dấu ấn sinh học bao gồm proteomics liên quan đến bệnh lý thận và chứng đối chứng bình thường đã được thực hiện. Phân tích dữ liệu này cho thấy các con đường và dấu ấn sinh học đặc hiệu đối với các bệnh lý khác nhau như: AR, CR, IRI, TI, VR, DGF, AABMR, ACMR, độc tính thuốc ức chế miễn dịch và nhiễm trùng. Nó cũng tiết lộ các dấu ấn sinh học được đề xuất để phát hiện tốt hơn các bệnh lý này và sức mạnh (độ nhạy và độ đặc hiệu) của các dấu ấn sinh học như vậy. Cuối cùng, lĩnh vực proteomics trong ghép thận về mặt phương pháp luận, những thách thức hiện tại trong chuyển dịch lâm sàng và các giải pháp khả thi cũng được thảo luận. Bình luận của chuyên gia: Phân tích các dấu ấn sinh học của tình trạng thải ghép cấp tính và mạn tính cho thấy thải ghép cấp tính có thể là một quá trình viêm nhiều hơn. So sánh các nghiên cứu dựa trên proteomic và protein (n = 183), phiên mã (n = 1) và giải trình tự gen (n = 4) cho thấy các phương pháp tiếp cận dựa trên proteomic và protein có thể cung cấp một bức tranh rõ ràng hơn về tình trạng viêm trong tình trạng thải ghép cấp tính."} +{"idx": 1930058, "en": "OBJECTIVES To comparein vitro differences in the apical filling regarding working length (WL) change and presence of voids and to validate optical coherence tomography (OCT) in comparison with computerized microtomography (µCT) for the detection of failures in the apical filling. METHODS Forty-five uniradicular teeth with round canals, divided into groups (n = 15) following the obturation protocols: LC (lateral condensation), TMC (thermomechanical compaction) and SC (single cone). Samples were scanned using µCT (parameters: 80 kV, 222 µA, and resolution of 11 µm), OCT (parameters: SSOCT, 1300 nm and axial resolution of 12 µm), and periapical digital radiography. The images were analyzsed by two blind and calibrated observers using ImageJ software to measure the boundary of the obturation WL and voids presence. Categorical and metric data were submitted to inferential analysis, and the validity of the OCT as a diagnostic test was assessed with performance and reliability tests. RESULTS The WL average remained constant for all obturation techniques and image methods. OCT showed adequate sensitivity and specificity to detect voids in the WL of apical obturations in vitro in comparison with µCT. Both image methods found a higher number of voids for LC technique (µCT p = 0.011/OCT p = 0.002). CONCLUSIONS OCT can be used in apical obturation voids assessment and the LC technique revealed more voids with larger dimensions.", "gemini": "MỤC TIÊU So sánh sự khác biệt in vitro trong việc trám bít đỉnh liên quan đến sự thay đổi chiều dài làm việc (WL) và sự hiện diện của các khoảng trống và để xác nhận tính hợp lệ của phương pháp chụp cắt lớp quang học (OCT) so với phương pháp chụp cắt lớp vi tính (µCT) để phát hiện các lỗ hổng trong việc trám bít đỉnh. PHƯƠNG PHÁP Bốn mươi lăm răng một chân có ống tủy tròn, được chia thành các nhóm (n = 15) theo các giao thức trám bít: LC (nén bên), TMC (nén nhiệt cơ) và SC (hình nón đơn). Các mẫu được quét bằng µCT (các thông số: 80 kV, 222 µA và độ phân giải 11 µm), OCT (các thông số: SSOCT, 1300 nm và độ phân giải trục 12 µm) và chụp X quang kỹ thuật số quanh chóp. Các hình ảnh được phân tích bởi hai người quan sát mù và được hiệu chuẩn bằng phần mềm ImageJ để đo ranh giới của WL trám bít và sự hiện diện của các khoảng trống. Dữ liệu phân loại và số liệu đã được gửi để phân tích suy luận và tính hợp lệ của OCT như một xét nghiệm chẩn đoán đã được đánh giá bằng các xét nghiệm hiệu suất và độ tin cậy. KẾT QUẢ WL trung bình vẫn không đổi đối với tất cả các kỹ thuật trám bít và phương pháp hình ảnh. OCT cho thấy độ nhạy và độ đặc hiệu đầy đủ để phát hiện các khoảng trống trong WL của các miếng trám bít đỉnh in vitro so với µCT. Cả hai phương pháp hình ảnh đều tìm thấy số lượng khoảng trống nhiều hơn đối với kỹ thuật LC (µCT p = 0,011 / OCT p = 0,002). KẾT LUẬN OCT có thể được sử dụng để đánh giá các khoảng trống trám bít đỉnh và kỹ thuật LC cho thấy nhiều khoảng trống hơn với kích thước lớn hơn."} +{"idx": 1930059, "en": "PURPOSE This article examines how theoretical and clinical applications of social network analysis (SNA) can inform opportunities for innovation and advancement of social support programming for adolescent and young adult (AYA) cancer patients and survivors. APPLICATIONS SNA can help address potential barriers and challenges to initiating and sustaining AYA peer support by helping to identify the diverse psychosocial needs among individuals in the AYA age range; find strategic ways to support and connect AYAs at different phases of the cancer trajectory with resources and services; and increase awareness of psychosocial resources and referrals from healthcare providers. Network perspectives on homophily, proximity, and evolution provide a foundational basis to explore the utility of SNA in AYA clinical care and research initiatives. The uniqueness of the AYA oncology community can also provide insight into extending and developing current SNA theories. IMPLICATIONS Using SNA in AYA psychosocial cancer research has the potential to create new ideas and pathways for supporting AYAs across the continuum of care, while also extending theories of SNA. SNA may also prove to be a useful tool for examining social support resources for AYAs with various chronic health conditions and other like groups.", "gemini": "MỤC ĐÍCH Bài viết này xem xét cách các ứng dụng lý thuyết và lâm sàng của phân tích mạng xã hội (SNA) có thể cung cấp thông tin về các cơ hội đổi mới và tiến bộ của chương trình hỗ trợ xã hội cho bệnh nhân ung thư và người sống sót ở thanh thiếu niên và thanh niên (AYA). ỨNG DỤNG SNA có thể giúp giải quyết các rào cản và thách thức tiềm ẩn đối với việc khởi xướng và duy trì hỗ trợ ngang hàng AYA bằng cách giúp xác định các nhu cầu tâm lý xã hội đa dạng giữa các cá nhân trong độ tuổi AYA; tìm ra những cách chiến lược để hỗ trợ và kết nối AYA ở các giai đoạn khác nhau của quá trình điều trị ung thư với các nguồn lực và dịch vụ; và nâng cao nhận thức về các nguồn lực tâm lý xã hội và các giới thiệu từ các nhà cung cấp dịch vụ chăm sóc sức khỏe. Quan điểm mạng lưới về sự đồng nhất, sự gần gũi và sự tiến hóa cung cấp cơ sở nền tảng để khám phá tiện ích của SNA trong các sáng kiến nghiên cứu và chăm sóc lâm sàng của AYA. Tính độc đáo của cộng đồng ung thư AYA cũng có thể cung cấp thông tin chi tiết để mở rộng và phát triển các lý thuyết SNA hiện tại. Ý NGHĨA Việc sử dụng SNA trong nghiên cứu ung thư tâm lý xã hội AYA có khả năng tạo ra những ý tưởng và con đường mới để hỗ trợ AYA trong suốt quá trình chăm sóc, đồng thời mở rộng các lý thuyết về SNA. SNA cũng có thể chứng minh là một công cụ hữu ích để kiểm tra các nguồn lực hỗ trợ xã hội cho AYA mắc các tình trạng sức khỏe mãn tính khác nhau và các nhóm tương tự khác."} +{"idx": 1930060, "en": "Universal vaccination programmes against Hepatitis B Virus (HBV) have significantly reduced the burden of the disease; nevertheless, HBV infection remains a relevant issue for high-risk subjects, such as healthcare workers (HCWs), who may potentially be exposed to blood or body fluids. Our study evaluates the long-term duration of the immunological memory of HBV vaccination 11-23 years after primary immunization by examining the response to booster doses in HCWs and students of health disciplines at Careggi Teaching Hospital in Florence (Italy). All participants (n = 2,203) had received a complete HBV immunization course in infancy or adolescence. Blood samples were collected to measure antibody levels against the HBV surface antigen (anti-HBs); an anti-HBs titre <10 mIU/mL was considered as negative. The administration of the vaccination course during infancy induced lower long-term anti-HBs titres compared to those in case of vaccination performed during adolescence (titre <10 mIU/mL: 51.1% and 12.2% respectively; p < 0.001), also considering that an equal number of years has elapsed since vaccination. A booster dose administered to subjects vaccinated in infancy is able to induce anamnestic immunological response in a higher percentage of vaccinated people (p < 0.001). Few subjects (n. = 4) accepted a fifth dose of vaccine in the case of persistent anti-HBs negative titres; this aspect requires further investigation. The total absence of acute hepatitis B among vaccinated subjects suggests that the long incubation period of the disease allows the activation of immunologic memory mechanisms, which is also true in case of low anti-HBs level. In conclusion HCWs still represent a high-risk category; it is therefore, necessary to increase efforts to protect and vaccinate these subjects.", "gemini": "Các chương trình tiêm chủng toàn cầu chống lại Vi-rút viêm gan B (HBV) đã làm giảm đáng kể gánh nặng của bệnh; tuy nhiên, nhiễm HBV vẫn là một vấn đề có liên quan đối với những đối tượng có nguy cơ cao, chẳng hạn như nhân viên y tế (HCW), những người có khả năng tiếp xúc với máu hoặc dịch cơ thể. Nghiên cứu của chúng tôi đánh giá thời gian kéo dài của trí nhớ miễn dịch của vắc-xin HBV 11-23 năm sau khi tiêm chủng ban đầu bằng cách kiểm tra phản ứng với liều tăng cường ở nhân viên y tế và sinh viên các ngành y tại Bệnh viện giảng dạy Careggi ở Florence (Ý). Tất cả những người tham gia (n = 2.203) đã được tiêm chủng HBV đầy đủ trong thời thơ ấu hoặc vị thành niên. Các mẫu máu được thu thập để đo mức kháng thể chống lại kháng nguyên bề mặt HBV (anti-HBs); nồng độ anti-HBs <10 mIU / mL được coi là âm tính. Việc tiêm chủng trong thời thơ ấu tạo ra nồng độ anti-HBs dài hạn thấp hơn so với trường hợp tiêm chủng trong thời vị thành niên (nồng độ <10 mIU / mL: lần lượt là 51,1% và 12,2%; p <0,001), cũng xem xét rằng một số năm bằng nhau đã trôi qua kể từ khi tiêm chủng. Một liều tăng cường được tiêm cho những đối tượng được tiêm chủng trong thời thơ ấu có thể gây ra phản ứng miễn dịch hồi tưởng ở tỷ lệ người được tiêm chủng cao hơn (p <0,001). Một số ít đối tượng (n. = 4) chấp nhận liều vắc-xin thứ năm trong trường hợp nồng độ anti-HBs âm tính liên tục; khía cạnh này cần được điều tra thêm. Việc không có hoàn toàn viêm gan B cấp tính ở những đối tượng được tiêm chủng cho thấy thời gian ủ bệnh dài của bệnh cho phép kích hoạt các cơ chế miễn dịch học, điều này cũng đúng trong trường hợp nồng độ anti-HBs thấp. Tóm lại, nhân viên y tế vẫn là nhóm có nguy cơ cao; do đó, cần phải tăng cường các nỗ lực để bảo vệ và tiêm chủng cho những đối tượng này."} +{"idx": 1930061, "en": "BACKGROUND Video-Assisted Thoracic Surgery (VATS) is conventionally performed through multiple small incisions (C-VATS). Recent studies have reported encouraging results with the single-incision VATS (S-VATS) over the conventional technique. However, these studies were either small in size, unfocused, nonuniform, retrospective, lacking follow-up information, or focused on pain. We aim to validate previously reported results in a single large meta-analysis, including only the best evidence studies available. METHODS Systematic review of the PubMed archive was conducted to include only full English articles with Newcastle Ottawa Scale score ≥7. The primary outcome was the complications rate while secondary outcomes were operative time, resected lymph nodes (LNs), chest tube duration, estimated blood loss, length of postoperative stay (LOS), and postoperative pain on day 1 after surgery. Odds ratio and standard mean difference were used as effect estimates. Random model and leave-one-out analysis were used. RESULTS A total of 39 studies were included with 4635 patients (1686 S-VATS versus 2949 C-VATS). S-VATS has resulted in significantly less postoperative pain (P < .001), blood loss (P = .006), LOS (P < .001), and chest tube duration (P < .001). In lung cancer patients, the number of retrieved LNs was similar to that of C-VATS (P > .05). Subgroup comparison of the rate of complications between lung resections versus other intrathoracic procedures, lung cancer versus pneumothorax, and lung cancer versus other lung-only lesions did not show any significant differences between the groups. CONCLUSION Performing S-VATS technique has shown superior postoperative outcomes over the C-VATS technique in the treatment of thoracic disorders. Substantial benefit was confirmed in terms of less postoperative pain, blood loss, drainage time, and postoperative hospital stay.", "gemini": "LÝ LỊCH Phẫu thuật nội soi lồng ngực hỗ trợ video (VATS) thường được thực hiện thông qua nhiều vết rạch nhỏ (C-VATS). Các nghiên cứu gần đây đã báo cáo kết quả khả quan với VATS một vết rạch (S-VATS) so với kỹ thuật thông thường. Tuy nhiên, những nghiên cứu này hoặc có quy mô nhỏ, không tập trung, không thống nhất, hồi cứu, thiếu thông tin theo dõi hoặc tập trung vào cơn đau. Chúng tôi đặt mục tiêu xác thực các kết quả đã báo cáo trước đây trong một phân tích tổng hợp lớn duy nhất, chỉ bao gồm các nghiên cứu bằng chứng tốt nhất hiện có. PHƯƠNG PHÁP Đánh giá có hệ thống kho lưu trữ PubMed đã được tiến hành để chỉ bao gồm các bài báo tiếng Anh đầy đủ với điểm số Thang điểm Newcastle Ottawa ≥7. Kết quả chính là tỷ lệ biến chứng trong khi kết quả phụ là thời gian phẫu thuật, hạch bạch huyết cắt bỏ (LN), thời gian đặt ống ngực, lượng máu mất ước tính, thời gian nằm viện sau phẫu thuật (LOS) và cơn đau sau phẫu thuật vào ngày 1 sau phẫu thuật. Tỷ lệ cược và chênh lệch trung bình chuẩn được sử dụng làm ước tính hiệu ứng. Mô hình ngẫu nhiên và phân tích loại bỏ một lần đã được sử dụng. KẾT QUẢ Tổng cộng có 39 nghiên cứu được đưa vào với 4635 bệnh nhân (1686 S-VATS so với 2949 C-VATS). S-VATS dẫn đến đau sau phẫu thuật ít hơn đáng kể (P <0,001), mất máu (P = 0,006), LOS (P <0,001) và thời gian đặt ống ngực (P <0,001). Ở bệnh nhân ung thư phổi, số lượng hạch bạch huyết lấy ra tương tự như C-VATS (P> 0,05). So sánh phân nhóm về tỷ lệ biến chứng giữa cắt bỏ phổi so với các thủ thuật nội ngực khác, ung thư phổi so với tràn khí màng phổi và ung thư phổi so với các tổn thương chỉ ở phổi khác không cho thấy bất kỳ sự khác biệt đáng kể nào giữa các nhóm. KẾT LUẬN Thực hiện kỹ thuật S-VATS đã cho thấy kết quả sau phẫu thuật vượt trội hơn kỹ thuật C-VATS trong điều trị các rối loạn về ngực. Lợi ích đáng kể đã được xác nhận về mặt giảm đau sau phẫu thuật, mất máu, thời gian dẫn lưu và thời gian nằm viện sau phẫu thuật."} +{"idx": 1930062, "en": "White matter hyperintensities (WMH) are associated with cognitive decline. We aimed to identify the spatial specificity of WMH impact on cognition in non-demented, healthy elderly. We quantified WMH volume among healthy participants of a community dwelling cohort ( n = 702, age range 60 - 82 years, mean age = 69.5 years, 46% female) and investigated the effects of WMH on cognition and behavior, specifically for executive function, memory, and motor speed performance. Lesion location influenced their effect on cognition and behavior: Frontal WMH in the proximity of the frontal ventricles mainly affected executive function and parieto-temporal WMH in the proximity of the posterior horns deteriorated memory, while WMH in the upper deep white matter-including the corticospinal tract-compromised motor speed performance. This study exposes the subtle and subclinical yet detrimental effects of WMH on cognition in healthy elderly, and strongly suggests a causal influence of WMH on cognition by demonstrating the spatial specificity of these effects.", "gemini": "Tăng cường độ tín hiệu chất trắng (WMH) có liên quan đến suy giảm nhận thức. Chúng tôi nhằm mục đích xác định tính đặc hiệu về không gian của tác động WMH đối với nhận thức ở người cao tuổi khỏe mạnh, không mắc chứng mất trí. Chúng tôi định lượng thể tích WMH trong số những người tham gia khỏe mạnh của một nhóm đối tượng cư trú trong cộng đồng (n = 702, độ tuổi từ 60 - 82, tuổi trung bình = 69,5, 46% là nữ) và điều tra tác động của WMH đối với nhận thức và hành vi, đặc biệt là đối với chức năng điều hành, trí nhớ và hiệu suất tốc độ vận động. Vị trí tổn thương ảnh hưởng đến tác động của chúng đối với nhận thức và hành vi: WMH ở vùng trán gần não thất chủ yếu ảnh hưởng đến chức năng điều hành và WMH ở vùng thái dương đỉnh gần các sừng sau làm suy giảm trí nhớ, trong khi WMH ở chất trắng sâu phía trên - bao gồm cả đường dẫn corticospinal - làm giảm hiệu suất tốc độ vận động. Nghiên cứu này phơi bày những tác động tinh tế và cận lâm sàng nhưng có hại của WMH đối với nhận thức ở người cao tuổi khỏe mạnh và cho thấy một cách mạnh mẽ ảnh hưởng nhân quả của WMH đối với nhận thức bằng cách chứng minh tính đặc hiệu về không gian của những tác động này."} +{"idx": 1930063, "en": "PURPOSE The aims of this review are to determine the feasibility of near-infrared fluorescence (NIRF) angiography in anastomotic colorectal surgery and to determine the effectiveness of the technique in improving imaging and quantification of vascularization, thereby aiding in decision making as to where to establish the anastomosis. METHODS A systematic literature search of PubMed and EMBASE was conducted. Searching through the reference lists of selected articles identified additional studies. All English language articles presenting original patient data regarding intraoperative NIRF angiography were included without restriction of type of study, except for case reports, technical notes, and video vignettes. The intervention consisted of intraoperative NIRF angiography during anastomotic colorectal surgery to assess perfusion of the colon, sigmoid, and/or rectum. Primary outcome parameters included ease of use, added surgical time, complications related to the technique, and costs. Other relevant outcomes were whether this technique changed intraoperative decision making, whether effort was taken by the authors to quantify the signal and the incidence of postoperative complications. RESULTS Ten studies were included. Eight of these studies make a statement about the ease of use. In none of the studies complications due to the use of the technique occurred. The technique changed the resection margin in 10.8% of all NIRF cases. The anastomotic leak rate was 3.5% in the NIRF group and 7.4% in the group with conventional imaging. Two of the included studies used an objective quantification of the fluorescence signal and perfusion, using ROIs (Hamamatsu Photonics) and IC-Calc® respectively. CONCLUSIONS Although the feasibility of the technique seems to be agreed on by all current research, large clinical trials are mandatory to further evaluate the added value of the technique.", "gemini": "MỤC ĐÍCH Mục đích của bài tổng quan này là xác định tính khả thi của chụp mạch huỳnh quang gần hồng ngoại (NIRF) trong phẫu thuật nối đại tràng - trực tràng và xác định hiệu quả của kỹ thuật này trong việc cải thiện hình ảnh và định lượng mạch máu, do đó hỗ trợ trong việc đưa ra quyết định về vị trí tạo nối. PHƯƠNG PHÁP Đã tiến hành tìm kiếm tài liệu có hệ thống trên PubMed và EMBASE. Việc tìm kiếm thông qua danh sách tham khảo của các bài báo đã chọn đã xác định thêm các nghiên cứu. Tất cả các bài báo tiếng Anh trình bày dữ liệu bệnh nhân ban đầu liên quan đến chụp mạch NIRF trong khi phẫu thuật đều được đưa vào mà không hạn chế loại nghiên cứu, ngoại trừ báo cáo trường hợp, ghi chú kỹ thuật và video minh họa. Can thiệp bao gồm chụp mạch NIRF trong khi phẫu thuật nối đại tràng - trực tràng để đánh giá tưới máu của đại tràng, đại tràng sigma và/hoặc trực tràng. Các thông số kết quả chính bao gồm tính dễ sử dụng, thời gian phẫu thuật bổ sung, các biến chứng liên quan đến kỹ thuật và chi phí. Các kết quả có liên quan khác là liệu kỹ thuật này có thay đổi quyết định trong khi phẫu thuật hay không, liệu các tác giả có nỗ lực định lượng tín hiệu và tỷ lệ biến chứng sau phẫu thuật hay không. KẾT QUẢ Có mười nghiên cứu được đưa vào. Tám trong số các nghiên cứu này đưa ra tuyên bố về tính dễ sử dụng. Trong không có nghiên cứu nào xảy ra biến chứng do sử dụng kỹ thuật. Kỹ thuật này đã thay đổi đường ranh cắt bỏ trong 10,8% tổng số trường hợp NIRF. Tỷ lệ rò nối là 3,5% ở nhóm NIRF và 7,4% ở nhóm chụp ảnh thông thường. Hai trong số các nghiên cứu được đưa vào sử dụng định lượng khách quan tín hiệu huỳnh quang và tưới máu, sử dụng ROI (Hamamatsu Photonics) và IC-Calc® tương ứng. KẾT LUẬN Mặc dù tính khả thi của kỹ thuật này dường như được tất cả các nghiên cứu hiện tại nhất trí, nhưng các thử nghiệm lâm sàng lớn là bắt buộc để đánh giá thêm giá trị gia tăng của kỹ thuật này."} +{"idx": 1930064, "en": "The role of transcription factors (TFs) on nucleosome positioning, RNA polymerase recruitment, and transcription initiation has been extensively characterized. Here, we propose that a subset of TFs such as Reb1, Abf1, Rap1, and TFIIIB also serve a major function in partitioning transcription units by assisting the Nrd1p-Nab3p-Sen1p Pol II termination pathway.", "gemini": "Vai trò của các yếu tố phiên mã (TF) đối với vị trí nucleosome, tuyển dụng RNA polymerase và khởi đầu phiên mã đã được mô tả chi tiết. Ở đây, chúng tôi đề xuất rằng một nhóm TF như Reb1, Abf1, Rap1 và TFIIIB cũng đóng vai trò chính trong việc phân vùng các đơn vị phiên mã bằng cách hỗ trợ con đường kết thúc Pol II của Nrd1p-Nab3p-Sen1p."} +{"idx": 1930065, "en": "Crosstalk among brain endothelial cells (BECs), pericytes, and astrocytes occurs by way of soluble factors, including cytokines. Here, we studied cytokine secretion from both mouse BEC monocultures and tri-cultured with pericytes and astrocytes. Four cytokines were constitutively secreted by BEC monolayers, 12 by LPS-stimulated BECs, 10 by tri-cultures, and 14 by LPS-stimulated tri-cultures. Cytokine levels were generally higher with either LPS stimulation or tri-culture when compared to monocultures and highest in tri-cultures stimulated by LPS. LPS-stimulated secretions fell into eight patterns as categorized by the polarization of cytokine secretions. To determine the cellular origin of cytokine increases in tri-cultures, we cultured mouse BECs with human pericytes and astrocytes and measured cytokines in species-specific assays. Thus, cytokines detected in the human immunoassay were from pericytes/astrocytes and those detected in the mouse immunoassay were from BECs. Several unique patterns were thus found. For example, TNF-alpha was only of pericyte/astrocyte origin; granulocyte colony-stimulating factor was only of BEC origin; IL-6, MCP-1, and GM-CSF of astrocyte/pericyte origin were found in both the luminal and abluminal chambers, suggesting the presence of brain-to-blood transporters. We conclude that crosstalk influences cytokine secretion under constitutive and stimulated conditions from both BECs and pericytes/astrocytes.", "gemini": "Sự tương tác giữa các tế bào nội mô não (BEC), tế bào quanh mạch và tế bào hình sao xảy ra thông qua các yếu tố hòa tan, bao gồm cytokine. Ở đây, chúng tôi nghiên cứu sự tiết cytokine từ cả hai loại đơn bào BEC của chuột và ba loại được nuôi cấy với tế bào quanh mạch và tế bào hình sao. Bốn cytokine được tiết ra liên tục bởi các lớp đơn BEC, 12 cytokine được tiết ra bởi các BEC được kích thích bằng LPS, 10 cytokine được tiết ra bởi các loại nuôi cấy ba loại và 14 cytokine được tiết ra bởi các loại nuôi cấy ba loại được kích thích bằng LPS. Nồng độ cytokine thường cao hơn khi kích thích bằng LPS hoặc nuôi cấy ba loại khi so sánh với nuôi cấy đơn loại và cao nhất ở các loại nuôi cấy ba loại được kích thích bằng LPS. Các chất tiết được kích thích bằng LPS được chia thành tám kiểu theo phân loại phân cực của các chất tiết cytokine. Để xác định nguồn gốc tế bào của sự gia tăng cytokine trong các loại nuôi cấy ba loại, chúng tôi đã nuôi cấy các BEC của chuột với các tế bào quanh mạch và tế bào hình sao của người và đo cytokine trong các xét nghiệm đặc hiệu theo loài. Do đó, các cytokine được phát hiện trong xét nghiệm miễn dịch của người là từ các tế bào quanh mạch/tế bào hình sao và các cytokine được phát hiện trong xét nghiệm miễn dịch của chuột là từ BEC. Do đó, một số kiểu mẫu độc đáo đã được tìm thấy. Ví dụ, TNF-alpha chỉ có nguồn gốc từ tế bào quanh mạch/tế bào hình sao; yếu tố kích thích tạo khuẩn lạc hạt chỉ có nguồn gốc từ BEC; IL-6, MCP-1 và GM-CSF có nguồn gốc từ tế bào hình sao/tế bào quanh mạch được tìm thấy ở cả khoang trong lòng mạch và ngoài lòng mạch, cho thấy sự có mặt của các chất vận chuyển từ não đến máu. Chúng tôi kết luận rằng sự tương tác ảnh hưởng đến sự tiết cytokine trong điều kiện cấu thành và được kích thích từ cả BEC và tế bào quanh mạch/tế bào hình sao."} +{"idx": 1930066, "en": "Five distinct riboswitch classes that regulate gene expression in response to the cofactor S-adenosylmethionine (SAM) or its metabolic breakdown product S-adenosylhomocysteine (SAH) have been reported previously. Collectively, these SAM- or SAH-sensing RNAs constitute the most abundant collection of riboswitches, and are found in nearly every major bacterial lineage. Here, we report a potential sixth member of this pervasive riboswitch family, called SAM-VI, which is predominantly found in Bifidobacterium species. SAM-VI aptamers selectively bind the cofactor SAM and strongly discriminate against SAH. The consensus sequence and structural model for SAM-VI share some features with the consensus model for the SAM-III riboswitch class, whose members are mainly found in lactic acid bacteria. However, there are sufficient differences between the two classes such that current bioinformatics methods separately cluster representatives of the two motifs. These findings highlight the abundance of RNA structures that can form to selectively recognize SAM, and showcase the ability of RNA to utilize diverse strategies to perform similar biological functions.", "gemini": "Năm lớp riboswitch riêng biệt điều hòa biểu hiện gen để đáp ứng với cofactor S-adenosylmethionine (SAM) hoặc sản phẩm phân hủy trao đổi chất của nó là S-adenosylhomocysteine (SAH) đã được báo cáo trước đây. Tổng hợp lại, các RNA cảm biến SAM hoặc SAH này tạo nên bộ sưu tập riboswitch phong phú nhất và được tìm thấy ở hầu hết mọi dòng vi khuẩn chính. Ở đây, chúng tôi báo cáo một thành viên thứ sáu tiềm năng của họ riboswitch phổ biến này, được gọi là SAM-VI, chủ yếu được tìm thấy ở các loài Bifidobacterium. Các aptamer SAM-VI liên kết chọn lọc với cofactor SAM và phân biệt mạnh với SAH. Trình tự đồng thuận và mô hình cấu trúc của SAM-VI có một số đặc điểm giống với mô hình đồng thuận của lớp riboswitch SAM-III, các thành viên chủ yếu được tìm thấy trong vi khuẩn axit lactic. Tuy nhiên, có đủ sự khác biệt giữa hai lớp sao cho các phương pháp tin sinh học hiện tại phân cụm riêng biệt các đại diện của hai mô típ. Những phát hiện này làm nổi bật sự phong phú của các cấu trúc RNA có thể hình thành để nhận dạng SAM một cách chọn lọc và thể hiện khả năng của RNA trong việc sử dụng các chiến lược đa dạng để thực hiện các chức năng sinh học tương tự."} +{"idx": 1930067, "en": "BACKGROUND Specific allergen immunotherapy to Hymenoptera venom (VIT) is a basic treatment for patients allergic to Hymenoptera venom. The aim of the study was to evaluate the safety of an ultra-rush regimen compared with the rush and conventional protocols. MATERIALS AND METHODS In 31 patients with an allergy to bee venom and 82 with an allergy to wasp venom, the allergic adverse reactions during VIT were monitored. Patients were selected based on the criteria established by EAACI (European Academy of Allergy and Clinical Immunology) recommendations. Adverse reactions during the ultra-rush immunotherapy were measured, documented and classified according to the criteria of Mueller. Ultra-rush, rush or conventional protocols of the initial phase VIT using the Venomenhal vaccine (Hal Allergy, Leiden, Netherlands) were conducted. RESULTS Six (13.7%) patients on the ultra-rush regimen, 5 (14.3%) patients on the rush regimen and 9 (26.5%) on conventional VIT experienced an allergic reaction. There were no associations between the adverse allergic reactions and the following factors: gender, total IgE and allergen-specific IgE to wasp or bee venom before the VIT and cardiological drugs that were used. CONCLUSION We found that the ultra-rush protocol (similar to the rush protocol) using the Venomenhal vaccine is safer than the conventional protocol.", "gemini": "LÝ LỊCH Miễn dịch trị liệu dị ứng đặc hiệu với nọc Hymenoptera (VIT) là một phương pháp điều trị cơ bản cho những bệnh nhân bị dị ứng với nọc Hymenoptera. Mục đích của nghiên cứu này là đánh giá độ an toàn của phác đồ siêu nhanh so với phác đồ nhanh và thông thường. VẬT LIỆU VÀ PHƯƠNG PHÁP Ở 31 bệnh nhân bị dị ứng với nọc ong và 82 bệnh nhân bị dị ứng với nọc ong đất, các phản ứng bất lợi do dị ứng trong quá trình VIT đã được theo dõi. Bệnh nhân được lựa chọn dựa trên các tiêu chuẩn do khuyến nghị của EAACI (Học viện Dị ứng và Miễn dịch học lâm sàng Châu Âu) đưa ra. Các phản ứng bất lợi trong quá trình miễn dịch trị liệu siêu nhanh đã được đo lường, ghi lại và phân loại theo tiêu chuẩn của Mueller. Các phác đồ siêu nhanh, nhanh hoặc thông thường của giai đoạn đầu VIT sử dụng vắc-xin Venomenhal (Hal Allergy, Leiden, Hà Lan) đã được tiến hành. KẾT QUẢ Sáu (13,7%) bệnh nhân trong phác đồ siêu nhanh, 5 (14,3%) bệnh nhân trong phác đồ nhanh và 9 (26,5%) trong VIT thông thường đã gặp phải phản ứng dị ứng. Không có mối liên quan nào giữa các phản ứng dị ứng bất lợi và các yếu tố sau: giới tính, tổng IgE và IgE đặc hiệu với chất gây dị ứng đối với nọc ong hoặc ong đất trước VIT và các loại thuốc tim mạch đã sử dụng. KẾT LUẬN Chúng tôi thấy rằng phác đồ siêu nhanh (tương tự như phác đồ nhanh) sử dụng vắc-xin Venomenhal an toàn hơn phác đồ thông thường."} +{"idx": 1930068, "en": "The discovery of microbial fibrinolytic enzymes is essential to treat cardiovascular diseases. This study reports the discovery of a fibrinolytic enzyme secreted by Bacillus cereus SRM-001, a microorganism isolated from the soil of a chicken waste-dump yard. The B. cereus SRM-001 was cultured and the secreted fibrinolytic enzyme purified to show that it is a ∼28 kDa protein. The purified enzyme was characterized for its kinetics, biochemical and thermal properties to show that it possesses properties similar to plasmin. A HPLC-MS/MS analysis of trypsin digested protein indicated that the fibrinolytic enzyme shared close sequence homology with serine proteases reported for other Bacillus sp. The results show that the B. cereus SRM-001 secreted enzyme is a ∼28 kDa serine protease that possesses fibrinolytic potential.", "gemini": "Việc khám phá ra các enzyme phân giải fibrin của vi khuẩn là rất cần thiết để điều trị các bệnh tim mạch. Nghiên cứu này báo cáo về việc phát hiện ra một enzyme phân giải fibrin do Bacillus cereus SRM-001 tiết ra, một vi sinh vật được phân lập từ đất của bãi chứa chất thải của gà. Bacillus cereus SRM-001 đã được nuôi cấy và enzyme phân giải fibrin tiết ra được tinh chế để chứng minh rằng đó là một loại protein ∼28 kDa. Enzyme tinh khiết được đặc trưng về động học, tính chất sinh hóa và nhiệt để chứng minh rằng nó sở hữu các tính chất tương tự như plasmin. Phân tích HPLC-MS / MS của protein tiêu hóa bằng trypsin cho thấy enzyme phân giải fibrin có trình tự đồng đẳng chặt chẽ với protease serine được báo cáo cho các loài Bacillus khác. Kết quả cho thấy enzyme tiết ra của B. cereus SRM-001 là một protease serine ∼28 kDa có tiềm năng phân giải fibrin."} +{"idx": 1930069, "en": "BACKGROUND The presence of asymptomatic spinal cord (SC) lesions in patients with clinically isolated syndrome (CIS) or relapsing-remitting multiple sclerosis (RRMS) predicts conversion to clinically definite multiple sclerosis (CDMS). The relation between asymptomatic SC abnormalities and disability progression warrants further investigation. OBJECTIVE To determine the prognostic value of asymptomatic SC lesions in CIS and early RRMS with respect to the time to disability development. METHODS Clinical and demographic data, brain and SC magnetic resonance imaging (MRI) were collected of CIS or early RRMS patients. Two main analyses were performed. For the first analysis, patients were divided into two groups: (1) patients with asymptomatic SC lesions and (2) patients without SC lesions and patients with symptomatic SC lesions. The second analysis excluded patients with symptomatic SC lesions. Incidence curves were used to analyse differences between these groups in time to the development of disability and time to a second relapse. RESULTS A total of 178 patients were included, and 42 patients (23.6%) had asymptomatic SC lesions. No significant differences were found on the time to disability development or the time to a second event. CONCLUSION Asymptomatic SC lesions early in the disease course do not predict the time to disability development in patients diagnosed with CIS or early RRMS.", "gemini": "BỐI CẢNH Sự hiện diện của các tổn thương tủy sống (SC) không triệu chứng ở những bệnh nhân mắc hội chứng biệt lập lâm sàng (CIS) hoặc đa xơ cứng tái phát-giảm nhẹ (RRMS) dự đoán chuyển sang đa xơ cứng lâm sàng (CDMS). Mối quan hệ giữa các bất thường SC không triệu chứng và tiến triển khuyết tật cần được điều tra thêm. MỤC TIÊU Xác định giá trị tiên lượng của các tổn thương SC không triệu chứng ở CIS và RRMS sớm liên quan đến thời gian phát triển khuyết tật. PHƯƠNG PHÁP Dữ liệu lâm sàng và nhân khẩu học, hình ảnh cộng hưởng từ não và SC (MRI) đã được thu thập từ bệnh nhân CIS hoặc RRMS sớm. Hai phân tích chính đã được thực hiện. Đối với phân tích đầu tiên, bệnh nhân được chia thành hai nhóm: (1) bệnh nhân có tổn thương SC không triệu chứng và (2) bệnh nhân không có tổn thương SC và bệnh nhân có tổn thương SC có triệu chứng. Phân tích thứ hai loại trừ những bệnh nhân có tổn thương SC có triệu chứng. Đường cong tỷ lệ mắc đã được sử dụng để phân tích sự khác biệt giữa các nhóm này về thời gian phát triển khuyết tật và thời gian tái phát lần thứ hai. KẾT QUẢ Tổng cộng có 178 bệnh nhân được đưa vào và 42 bệnh nhân (23,6%) có tổn thương SC không triệu chứng. Không tìm thấy sự khác biệt đáng kể về thời gian phát triển khuyết tật hoặc thời gian đến lần thứ hai. KẾT LUẬN Các tổn thương SC không triệu chứng ở giai đoạn đầu của quá trình bệnh không dự đoán thời gian phát triển khuyết tật ở những bệnh nhân được chẩn đoán mắc CIS hoặc RRMS sớm."} +{"idx": 1930070, "en": "The North American Imaging in Multiple Sclerosis (NAIMS) Cooperative represents a network of 27 academic centers focused on accelerating the pace of magnetic resonance imaging (MRI) research in multiple sclerosis (MS) through idea exchange and collaboration. Recently, NAIMS completed its first project evaluating the feasibility of implementation and reproducibility of quantitative MRI measures derived from scanning a single MS patient using a high-resolution 3T protocol at seven sites. The results showed the feasibility of utilizing advanced quantitative MRI measures in multicenter studies and demonstrated the importance of careful standardization of scanning protocols, central image processing, and strategies to account for inter-site variability.", "gemini": "Hệ thống Chẩn đoán hình ảnh Bệnh đa xơ cứng Bắc Mỹ (NAIMS) là mạng lưới gồm 27 trung tâm học thuật tập trung vào việc đẩy nhanh tiến độ nghiên cứu hình ảnh cộng hưởng từ (MRI) về bệnh đa xơ cứng (MS) thông qua trao đổi ý tưởng và hợp tác. Gần đây, NAIMS đã hoàn thành dự án đầu tiên đánh giá tính khả thi của việc triển khai và khả năng tái tạo các biện pháp MRI định lượng thu được từ việc quét một bệnh nhân MS duy nhất bằng giao thức 3T độ phân giải cao tại bảy địa điểm. Kết quả cho thấy tính khả thi của việc sử dụng các biện pháp MRI định lượng tiên tiến trong các nghiên cứu đa trung tâm và chứng minh tầm quan trọng của việc chuẩn hóa cẩn thận các giao thức quét, xử lý hình ảnh trung tâm và các chiến lược để tính đến sự thay đổi giữa các địa điểm."} +{"idx": 1930071, "en": "The association of protein aggregates with plentiful human diseases has fascinated studies regarding the biophysical characterization of protein misfolding and ultimately their aggregate formation mechanism. Protein-ligand interaction, their mechanism, conformational changes by ligands, and protein aggregate formation have been studied upon exploiting experimental techniques and computational methodologies. Such studies for the exploration of ligand-induced conformational changes in protein, misfolding and aggregation, has confirmed drastic progresses in the study of aggregate formation pathways. This review comprises of an inclusive description of contemporary experimental techniques as well as theoretical improvements in the interpretation of the conformational properties of protein. We have also discussed various factors responsible for the microenvironment change around protein that sequentially causes amyloidoses. Biophysical techniques and cell-based assays to gain comprehensive understandings of protein-ligand interaction, protein folding, and aggregation pathways have also been described. The promising therapeutic methods used to inhibit the protein fibrillogenesis have also been discussed.", "gemini": "Sự liên kết của các tập hợp protein với nhiều bệnh ở người đã hấp dẫn các nghiên cứu về đặc điểm sinh lý của sự sai lệch cấu trúc protein và cuối cùng là cơ chế hình thành tập hợp của chúng. Tương tác protein-ligand, cơ chế của chúng, sự thay đổi cấu hình do các phối tử và sự hình thành tập hợp protein đã được nghiên cứu khi khai thác các kỹ thuật thực nghiệm và phương pháp tính toán. Các nghiên cứu như vậy để khám phá các thay đổi cấu hình do phối tử gây ra trong protein, sự sai lệch cấu trúc và tập hợp, đã xác nhận những tiến bộ vượt bậc trong nghiên cứu về các con đường hình thành tập hợp. Đánh giá này bao gồm mô tả toàn diện về các kỹ thuật thực nghiệm đương đại cũng như những cải tiến lý thuyết trong việc giải thích các tính chất cấu hình của protein. Chúng tôi cũng đã thảo luận về các yếu tố khác nhau chịu trách nhiệm cho sự thay đổi vi môi trường xung quanh protein gây ra bệnh amyloidosis theo trình tự. Các kỹ thuật sinh lý và xét nghiệm dựa trên tế bào để có được sự hiểu biết toàn diện về tương tác protein-ligand, quá trình gấp nếp protein và các con đường tập hợp cũng đã được mô tả. Các phương pháp điều trị đầy hứa hẹn được sử dụng để ức chế quá trình tạo sợi protein cũng đã được thảo luận."} +{"idx": 1930072, "en": "Recent bioinformatics studies have demonstrated a wide-spread occurrence of the hammerhead ribozyme (HHR) and similar small endonucleolytic RNA motifs in all domains of life. It is becoming increasingly evident that such ribozyme motifs participate in important genetic processes in diverse organisms. Although the HHR motif has been studied for more than three decades, only little is known about the consequences of ribozyme activity on gene expression. In the present study we analysed eight different naturally occurring HHR sequences in diverse genetic and organismal contexts. We investigated the influence of active ribozymes incorporated into mRNAs in mammalian, yeast and bacterial expression systems. The experiments show an unexpectedly high degree of organism-specific variability of ribozyme-mediated effects on gene expression. The presented findings demonstrate that ribozyme cleavage profoundly affect gene expression. However, the extent of this effect varies and depends strongly on the respective genetic context. The fast-cleaving type 3 HHRs [CChMVd(-) and sLTSV(-)] generally tended to cause the strongest effects on intracellular gene expression. The presented results are important in order to address potential functions of naturally occurring ribozymes in RNA processing and post-transcriptional regulation of gene expression. Additionally, our results are of interest for biotechnology and synthetic biology approaches that aim at the utilisation of self-cleaving ribozymes as widely applicable tools for controlling genetic processes.", "gemini": "Các nghiên cứu tin sinh học gần đây đã chứng minh sự xuất hiện rộng rãi của ribozym búa đầu (HHR) và các mô típ RNA nội phân tử nhỏ tương tự trong mọi phạm vi của sự sống. Ngày càng rõ ràng rằng các mô típ ribozym như vậy tham gia vào các quá trình di truyền quan trọng ở các sinh vật khác nhau. Mặc dù mô típ HHR đã được nghiên cứu trong hơn ba thập kỷ, nhưng người ta vẫn biết rất ít về hậu quả của hoạt động ribozym đối với biểu hiện gen. Trong nghiên cứu hiện tại, chúng tôi đã phân tích tám trình tự HHR tự nhiên khác nhau trong các bối cảnh di truyền và sinh vật khác nhau. Chúng tôi đã điều tra ảnh hưởng của các ribozym hoạt động được đưa vào mRNA trong các hệ thống biểu hiện của động vật có vú, nấm men và vi khuẩn. Các thí nghiệm cho thấy mức độ biến đổi đặc hiệu theo sinh vật cao bất ngờ của các hiệu ứng do ribozym trung gian gây ra đối với biểu hiện gen. Các phát hiện được trình bày chứng minh rằng sự cắt ribozym ảnh hưởng sâu sắc đến biểu hiện gen. Tuy nhiên, mức độ của hiệu ứng này thay đổi và phụ thuộc mạnh vào bối cảnh di truyền tương ứng. Các HHR loại 3 cắt nhanh [CChMVd(-) và sLTSV(-)] nói chung có xu hướng gây ra những hiệu ứng mạnh nhất đối với biểu hiện gen trong tế bào. Các kết quả được trình bày rất quan trọng để giải quyết các chức năng tiềm ẩn của các ribozym tự nhiên trong quá trình xử lý RNA và điều hòa biểu hiện gen sau phiên mã. Ngoài ra, kết quả của chúng tôi có ý nghĩa đối với các phương pháp tiếp cận công nghệ sinh học và sinh học tổng hợp nhằm vào việc sử dụng các ribozym tự cắt làm các công cụ có thể áp dụng rộng rãi để kiểm soát các quá trình di truyền."} +{"idx": 1930073, "en": "Effector proteins are exported to the interior of host cells by diverse plant pathogens. Many oomycete pathogens maintain large families of candidate effector genes, encoding proteins with a secretory leader followed by an RxLR motif. Although most of these genes are very divergent between oomycete species, several genes are conserved between Phytophthora species and Hyaloperonospora arabidopsidis, suggesting that they play important roles in pathogenicity. We describe a pair of conserved effector candidates, HaRxL23 and PsAvh73, from H. arabidopsidis and P. sojae respectively. We show that HaRxL23 is expressed early during infection of Arabidopsis. HaRxL23 triggers an ecotype-specific defense response in Arabidopsis, suggesting that it is recognized by a host surveillance protein. HaRxL23 and PsAvh73 can suppress pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) in Nicotiana benthamiana and effector-triggered immunity (ETI) in soybean. Transgenic Arabidopsis constitutively expressing HaRxL23 or PsAvh73 exhibit suppression of PTI and enhancement of bacterial and oomycete virulence. Together, our experiments demonstrate that these conserved oomycete RxLR effectors suppress PTI and ETI across diverse plant species.", "gemini": "Các protein tác động được vận chuyển đến bên trong tế bào vật chủ bởi nhiều loại mầm bệnh thực vật. Nhiều mầm bệnh nấm oomycete duy trì các họ lớn các gen tác động ứng viên, mã hóa các protein có một đầu dẫn tiết theo sau là một mô típ RxLR. Mặc dù hầu hết các gen này rất khác biệt giữa các loài nấm oomycete, nhưng một số gen được bảo tồn giữa các loài Phytophthora và Hyaloperonospora arabidopsidis, cho thấy rằng chúng đóng vai trò quan trọng trong khả năng gây bệnh. Chúng tôi mô tả một cặp ứng viên tác động được bảo tồn, HaRxL23 và PsAvh73, lần lượt từ H. arabidopsidis và P. sojae. Chúng tôi chỉ ra rằng HaRxL23 được biểu hiện sớm trong quá trình lây nhiễm Arabidopsis. HaRxL23 kích hoạt phản ứng phòng vệ đặc hiệu kiểu gen ở Arabidopsis, cho thấy rằng nó được một protein giám sát vật chủ nhận ra. HaRxL23 và PsAvh73 có thể ức chế khả năng miễn dịch do kiểu phân tử liên quan đến mầm bệnh (PAMP) gây ra (PTI) ở Nicotiana benthamiana và khả năng miễn dịch do tác nhân gây ra (ETI) ở đậu tương. Arabidopsis chuyển gen biểu hiện liên tục HaRxL23 hoặc PsAvh73 cho thấy sự ức chế PTI và tăng cường độc lực của vi khuẩn và nấm oomycete. Kết hợp lại, các thí nghiệm của chúng tôi chứng minh rằng các tác nhân RxLR của nấm oomycete được bảo tồn này ức chế PTI và ETI trên nhiều loài thực vật khác nhau."} +{"idx": 1930074, "en": "BACKGROUND Dimethyl fumarate (DMF) and its active metabolite monomethyl fumarate (MMF) effectively lead to reduction in disease relapses and active magnetic resonance imaging (MRI) lesions. DMF and MMF are known to be effective in modulating T- and B-cell responses; however, their effect on the phenotype and function of human myeloid dendritic cells (mDCs) is not fully understood. OBJECTIVE To investigate the role of MMF on human mDCs maturation and function. METHODS mDCs from healthy controls were isolated and cultured in vitro with MMF. The effect of MMF on mDC gene expression was determined by polymerase chain reaction (PCR) array after in vitro MMF treatment. The ability of mDCs to activate T cells was assessed by in vitro co-culture system. mDCs from DMF-treated multiple sclerosis (MS) patients were analyzed by flow cytometry and PCR. RESULTS MMF treatment induced a less mature phenotype of mDCs with reduced expression of major histocompatibility complex class II (MHC-II), co-stimulatory molecules CD86, CD40, CD83, and expression of nuclear factor κB (NF-κB) subunits RELA and RELB. mDCs from DMF-treated MS patients also showed the same immature phenotype. T cells co-cultured with MMF-treated mDCs showed reduced proliferation with decreased production of interferon gamma (IFN-γ), interleukin-17 (IL-17), and granulocyte-macrophage colony-stimulating factor (GM-CSF) compared to untreated cells. CONCLUSION We report that MMF can modulate immune response by affecting human mDC function.", "gemini": "BỐI CẢNH Dimethyl fumarate (DMF) và chất chuyển hóa hoạt động của nó là monomethyl fumarate (MMF) có hiệu quả dẫn đến giảm tái phát bệnh và tổn thương cộng hưởng từ (MRI) hoạt động. DMF và MMF được biết là có hiệu quả trong việc điều chỉnh phản ứng của tế bào T và B; tuy nhiên, tác dụng của chúng đối với kiểu hình và chức năng của các tế bào樹 phỏng hạt tủy (mDC) của người vẫn chưa được hiểu đầy đủ. MỤC TIÊU Nghiên cứu vai trò của MMF đối với quá trình trưởng thành và chức năng của mDC ở người. PHƯƠNG PHÁP Các mDC từ những người đối chứng khỏe mạnh được phân lập và nuôi cấy trong ống nghiệm với MMF. Tác dụng của MMF đối với biểu hiện gen của mDC được xác định bằng mảng phản ứng chuỗi polymerase (PCR) sau khi xử lý MMF trong ống nghiệm. Khả năng kích hoạt tế bào T của mDC được đánh giá bằng hệ thống đồng nuôi cấy trong ống nghiệm. Các mDC từ bệnh nhân đa xơ cứng (MS) được điều trị bằng DMF được phân tích bằng phương pháp đo lưu lượng tế bào và PCR. KẾT QUẢ Xử lý MMF gây ra kiểu hình ít trưởng thành hơn của mDC với biểu hiện giảm phức hợp tương thích mô chính loại II (MHC-II), các phân tử đồng kích thích CD86, CD40, CD83 và biểu hiện các tiểu đơn vị RELA và RELB của nhân tố hạt nhân κB (NF-κB). Các mDC từ bệnh nhân MS được điều trị bằng DMF cũng cho thấy cùng kiểu hình chưa trưởng thành. Các tế bào T được đồng nuôi cấy với các mDC được xử lý bằng MMF cho thấy sự gia tăng sinh sản giảm với sự sản xuất giảm interferon gamma (IFN-γ), interleukin-17 (IL-17) và yếu tố kích thích tập đoàn đại thực bào hạt (GM-CSF) so với các tế bào chưa được xử lý. KẾT LUẬN Chúng tôi báo cáo rằng MMF có thể điều chỉnh phản ứng miễn dịch bằng cách ảnh hưởng đến chức năng mDC của người."} +{"idx": 1930075, "en": "Previously, we showed that oral application of the environmental pollutant dibenzo[a,l]pyrene (DB[a,l]P) induces oral tumors in mice. Thus, in the present investigation we examined the effect of alcohol on DB[a,l]P-induced DNA damage and immune regulation; we showed that alcohol (6.4% v/v in the diet, 35% of Calories) significantly enhanced the levels of (-)-anti-trans-DB[a,l]P-dA while decreased the levels of GSH in the mouse oral tissues. Analysis of RNA expression revealed that DB[a,l]P alone upregulates inflammatory genes while alcohol suppresses several markers of immune surveillance. Collectively, these results suggest that alcohol may enhance oral carcinogenesis induced by DB[a,l]P.", "gemini": "Trước đây, chúng tôi đã chứng minh rằng việc sử dụng thuốc uống chất ô nhiễm môi trường dibenzo[a,l]pyren (DB[a,l]P) gây ra khối u ở miệng ở chuột. Do đó, trong nghiên cứu hiện tại, chúng tôi đã kiểm tra tác dụng của rượu đối với tổn thương DNA và điều hòa miễn dịch do DB[a,l]P gây ra; chúng tôi đã chứng minh rằng rượu (6,4% v/v trong chế độ ăn, 35% lượng calo) làm tăng đáng kể nồng độ (-)-anti-trans-DB[a,l]P-dA trong khi làm giảm nồng độ GSH trong các mô miệng của chuột. Phân tích biểu hiện RNA cho thấy riêng DB[a,l]P làm tăng các gen gây viêm trong khi rượu ức chế một số dấu hiệu của giám sát miễn dịch. Nhìn chung, những kết quả này cho thấy rượu có thể làm tăng quá trình gây ung thư ở miệng do DB[a,l]P gây ra."} +{"idx": 1930076, "en": "In 1977, I fell in love at first sight, and since then eating disorders have been a central part of my life. As I step down after 25 years as editor of Eating Disorders: The Journal of Treatment and Prevention, I am also saying goodbye to the eating disorders field and moving on to retirement and other areas of interest. In this Last Word article, I will write about my history with the journal and the field and use this forum to thank a few of the people I've known along the way.", "gemini": "Vào năm 1977, tôi đã phải lòng ngay từ cái nhìn đầu tiên và kể từ đó, các rối loạn ăn uống đã trở thành một phần trọng tâm trong cuộc sống của tôi. Khi tôi rời khỏi vị trí biên tập viên của Tạp chí điều trị và phòng ngừa rối loạn ăn uống sau 25 năm, tôi cũng nói lời tạm biệt với lĩnh vực rối loạn ăn uống và chuyển sang nghỉ hưu và các lĩnh vực khác mà tôi quan tâm. Trong bài viết Lời cuối này, tôi sẽ viết về lịch sử của mình với tạp chí và lĩnh vực này, đồng thời sử dụng diễn đàn này để cảm ơn một số người mà tôi đã quen biết trên chặng đường."} +{"idx": 1930077, "en": "Besides the influenza virus (IV), several other viruses are responsible for influenza-like illness (ILI). Although human parechoviruses (HPeVs) and enteroviruses (EVs) may impact on ILI, limited data on their epidemiological characteristics are available. During seven consecutive winter seasons (from 2010-2011 to 2016-2017), within the framework of an influenza surveillance system (InfluNet), 593 respiratory swabs were collected from children ≤5 years of age with ILIs. Molecular detection showed that 58.3 % of swabs were positive for at least one of the viruses under study: 46 % for IV, 13 % for EV and 5.4 % for HPeV. A single virus was identified in 51.3 % of samples while more than one virus was detected in 7 % of the samples. The risk of contracting IV was higher than the risk associated with EV, which in turn was higher than the risk of contracting HPeV. The risk of developing an IV infection was twofold greater in children >3 years than in those ≤3 years, who had higher risk of EV/HPeV infection. The frequency of EV/HPeV-positive swabs increased significantly during the 2016-2017 winter season compared to the previous six seasons. Sixteen EV genotypes were identified belonging to species A and B. HPeV-1 was the most frequently detected genotype, followed by -6 and -3. In this study, IV was mainly responsible for ILI, however EV and HPeV were also involved and particularly affected children ≤3 years of age. Influenza surveillance samples could provide us with valuable insight into the epidemiological features of viruses involved in ILI.", "gemini": "Ngoài virus cúm (IV), một số loại virus khác cũng gây ra bệnh giống cúm (ILI). Mặc dù parechovirus ở người (HPeV) và enterovirus (EV) có thể ảnh hưởng đến ILI, nhưng dữ liệu về đặc điểm dịch tễ học của chúng còn hạn chế. Trong bảy mùa đông liên tiếp (từ 2010-2011 đến 2016-2017), trong khuôn khổ của hệ thống giám sát cúm (InfluNet), 593 mẫu dịch tỵ hầu đã được thu thập từ trẻ em ≤5 tuổi mắc ILI. Phát hiện phân tử cho thấy 58,3% mẫu dịch tỵ hầu dương tính với ít nhất một trong các loại virus được nghiên cứu: 46% đối với IV, 13% đối với EV và 5,4% đối với HPeV. Một loại virus duy nhất được xác định trong 51,3% mẫu trong khi hơn một loại virus được phát hiện trong 7% mẫu. Nguy cơ mắc IV cao hơn nguy cơ liên quan đến EV, ngược lại, nguy cơ mắc EV cao hơn nguy cơ mắc HPeV. Nguy cơ nhiễm IV cao gấp đôi ở trẻ >3 tuổi so với trẻ ≤3 tuổi, những trẻ có nguy cơ nhiễm EV/HPeV cao hơn. Tần suất mẫu dịch tỵ hầu dương tính với EV/HPeV tăng đáng kể trong mùa đông 2016-2017 so với sáu mùa trước đó. Mười sáu kiểu gen EV đã được xác định thuộc loài A và B. HPeV-1 là kiểu gen được phát hiện thường xuyên nhất, tiếp theo là -6 và -3. Trong nghiên cứu này, IV chủ yếu gây ra ILI, tuy nhiên EV và HPeV cũng có liên quan và đặc biệt ảnh hưởng đến trẻ em ≤3 tuổi. Các mẫu giám sát cúm có thể cung cấp cho chúng ta cái nhìn sâu sắc có giá trị về các đặc điểm dịch tễ học của các loại virus liên quan đến ILI."} +{"idx": 1930078, "en": "Eating disorder prevention programs have been assessed up to 3 years post-implementation; however, their impact on long-term resilience is unclear. The current study assessed disordered eating and body dissatisfaction in 116 dancers from a professional ballet school 15 or more years following their participation in an effective selective prevention program. Dancers who attended the ballet school during and following the program endorsed fewer thoughts and behaviours associated with bulimia, had lower lifetime prevalence of laxative use, and showed a trend toward lower lifetime rates of vomiting to control weight than those who attended the ballet school prior to the intervention.", "gemini": "Các chương trình phòng ngừa rối loạn ăn uống đã được đánh giá trong vòng 3 năm sau khi thực hiện; tuy nhiên, tác động của chúng đối với khả năng phục hồi lâu dài vẫn chưa rõ ràng. Nghiên cứu hiện tại đã đánh giá tình trạng ăn uống rối loạn và không hài lòng về cơ thể ở 116 vũ công từ một trường ba lê chuyên nghiệp 15 năm trở lên sau khi tham gia một chương trình phòng ngừa có chọn lọc hiệu quả. Những vũ công theo học trường ba lê trong và sau chương trình đã chứng thực ít suy nghĩ và hành vi liên quan đến chứng cuồng ăn, tỷ lệ sử dụng thuốc nhuận tràng thấp hơn trong suốt cuộc đời và có xu hướng nôn để kiểm soát cân nặng thấp hơn những người theo học trường ba lê trước khi can thiệp."} +{"idx": 1930079, "en": "The 15-year follow-up study (Bar, Chassin & Dionne, 2017) suggests of the Dance School Ecological Prevention Program (Piran, 1999) that it may have had long-term effects on the prevention of bulimia. This pattern of findings is in line with the results of the older cohort in the original outcome evaluation study. If the results of the follow up study hold when the 15-year outcome evaluation study is compared to a control group from a similarly competitive ballet school, this would reinforce the cause of prevention in the field of sports and dance, if not beyond. It will further support the implementation of systemic changes in the social and educational environments of children towards the prevention of eating disorders.", "gemini": "Nghiên cứu theo dõi 15 năm (Bar, Chassin & Dionne, 2017) cho thấy Chương trình Phòng ngừa Sinh thái Trường khiêu vũ (Piran, 1999) có thể có tác dụng lâu dài trong việc ngăn ngừa chứng cuồng ăn. Mẫu phát hiện này phù hợp với kết quả của nhóm đối chứng lớn tuổi hơn trong nghiên cứu đánh giá kết quả ban đầu. Nếu kết quả của nghiên cứu theo dõi vẫn giữ nguyên khi nghiên cứu đánh giá kết quả 15 năm được so sánh với nhóm đối chứng từ một trường ba lê có tính cạnh tranh tương tự, thì điều này sẽ củng cố nguyên nhân phòng ngừa trong lĩnh vực thể thao và khiêu vũ, nếu không muốn nói là vượt ra ngoài. Nó sẽ tiếp tục hỗ trợ việc thực hiện các thay đổi mang tính hệ thống trong môi trường xã hội và giáo dục của trẻ em hướng tới mục tiêu phòng ngừa các rối loạn ăn uống."} +{"idx": 1930080, "en": "Acinetobacter lactucae and Acinetobacter dijkshoorniae were recently described as novel species, and both were reported to be closely related to Acinetobacter pittii. Because they were reviewed and published almost concurrently, their descriptions did not include a specific comparison between these two novel species. Genomic data were provided in both initial descriptions, which simplifies the comparisons. Genome comparisons based on in silico DNA-DNA hybridizations, average nucleotide identity and core genome phylogeny of the type strain genomes establish that these strains are conspecific. Based on the rules of priority, A. dijkshoorniae should be reclassified as a later heterotypic synonym of A. lactucae.", "gemini": "Acinetobacter lactucae và Acinetobacter dijkshoorniae gần đây đã được mô tả là loài mới và cả hai đều được báo cáo là có quan hệ họ hàng gần với Acinetobacter pittii. Vì chúng được xem xét và công bố gần như đồng thời nên mô tả của chúng không bao gồm sự so sánh cụ thể giữa hai loài mới này. Dữ liệu bộ gen được cung cấp trong cả hai mô tả ban đầu, điều này giúp đơn giản hóa các so sánh. So sánh bộ gen dựa trên lai ghép DNA-DNA trong ống nghiệm, danh tính nucleotide trung bình và phả hệ bộ gen lõi của bộ gen chủng loại thiết lập rằng các chủng này là đồng loại. Dựa trên các quy tắc về ưu tiên, A. dijkshoorniae nên được phân loại lại là một từ đồng nghĩa muộn hơn của A. lactucae."} +{"idx": 1930081, "en": "I founded Eating Disorders: The Journal of Treatment & Prevention 25 years ago, and its character, reputation, and success are highly attributable to Arnold Andersen, John Foreyt, and Margo Maine, the three original senior editors, whose perspectives follow in this article. Having made the decision to retire from the journal, I asked the senior editors to transition to the emeritus board along with me, which I explain further in my own Last Word, \"Goodbye, Eating Disorders,\" in this volume (Cohn, 2017). Over our 25 years of collaboration, the senior editors have acquired unique insights into the evolution of the eating disorders field; so, I asked them to contribute retrospective essays in which they also look forward within their primary areas of expertise-presented here in alphabetical order. - Leigh Cohn, Editor-in-Chief.", "gemini": "Tôi đã sáng lập tạp chí Eating Disorders: The Journal of Treatment & Prevention cách đây 25 năm, và tính cách, danh tiếng và thành công của nó có được là nhờ Arnold Andersen, John Foreyt và Margo Maine, ba biên tập viên cao cấp ban đầu, những người có quan điểm được trình bày trong bài viết này. Sau khi đưa ra quyết định nghỉ hưu khỏi tạp chí, tôi đã yêu cầu các biên tập viên cao cấp chuyển sang ban danh dự cùng với tôi, điều mà tôi sẽ giải thích thêm trong Lời cuối của riêng mình, \"Tạm biệt, Eating Disorders,\" trong tập này (Cohn, 2017). Trong hơn 25 năm hợp tác, các biên tập viên cao cấp đã có được những hiểu biết độc đáo về sự phát triển của lĩnh vực rối loạn ăn uống; vì vậy, tôi đã yêu cầu họ đóng góp các bài tiểu luận hồi cố trong đó họ cũng mong muốn hướng tới các lĩnh vực chuyên môn chính của mình - được trình bày ở đây theo thứ tự bảng chữ cái. - Leigh Cohn, Tổng biên tập."} +{"idx": 1930082, "en": "INTRODUCTION Children of newcomers to Canada have been identified as at moderately high risk for developing early childhood caries (ECC). The purpose of this study was to investigate the oral health of preschool children of refugee and immigrant families in Winnipeg. METHODS Children < 72 months of age and their parent or primary caregiver were recruited through several newcomer settlement agencies, dental clinics and community programs. Parents and caregivers completed a short questionnaire with the assistance of a research team member. Children underwent a dental examination. Results of the questionnaire were combined with those of the clinical examination and subjected to statistical analysis. RESULTS We recruited 211 children. Their mean age was 40.2 ± 15.4 months, and 54.0% were boys. Overall, 45.5% of the children had ECC and 31.8% had severe ECC (S-ECC). The mean decayed, missing and filled teeth (dmft) score was 2.2 ± 3.8 (range 0-19), while the mean decayed, missing, filled surfaces (dmfs) score was 4.8 ± 11.0 (range 0-63). Infant dental enucleation was observed in 6 children. Logistic regression analyses showed that increasing age, the presence of debris on teeth, parents believing their child has dental problems and the presence of enamel hypoplasia were significantly and independently associated with ECC and S-ECC (p ≤ 0.05). CONCLUSIONS ECC is prevalent in children of newcomer families in Manitoba. These data will inform advocacy efforts to improve access to dental care and tailor early childhood oral health promotion and ECC prevention activities for refugees and recent immigrants.", "gemini": "GIỚI THIỆU Trẻ em của những người mới đến Canada được xác định có nguy cơ mắc sâu răng ở trẻ em (ECC) ở mức độ trung bình. Mục đích của nghiên cứu này là để điều tra sức khỏe răng miệng của trẻ em mẫu giáo thuộc các gia đình tị nạn và nhập cư ở Winnipeg. PHƯƠNG PHÁP Trẻ em < 72 tháng tuổi và cha mẹ hoặc người chăm sóc chính của trẻ được tuyển dụng thông qua một số cơ quan định cư cho người mới đến, phòng khám nha khoa và các chương trình cộng đồng. Cha mẹ và người chăm sóc đã hoàn thành một bảng câu hỏi ngắn với sự hỗ trợ của một thành viên trong nhóm nghiên cứu. Trẻ em đã trải qua một cuộc kiểm tra nha khoa. Kết quả của bảng câu hỏi được kết hợp với kết quả của cuộc kiểm tra lâm sàng và được phân tích thống kê. KẾT QUẢ Chúng tôi đã tuyển dụng 211 trẻ em. Độ tuổi trung bình của chúng là 40,2 ± 15,4 tháng và 54,0% là bé trai. Nhìn chung, 45,5% trẻ em mắc ECC và 31,8% mắc ECC nghiêm trọng (S-ECC). Điểm số trung bình của răng sâu, mất và trám (dmft) là 2,2 ± 3,8 (phạm vi 0-19), trong khi điểm số trung bình của bề mặt sâu, mất, trám (dmfs) là 4,8 ± 11,0 (phạm vi 0-63). Tình trạng nhổ răng trẻ em được quan sát thấy ở 6 trẻ em. Phân tích hồi quy logistic cho thấy tuổi càng cao, có mảnh vụn trên răng, cha mẹ tin rằng con mình có vấn đề về răng và tình trạng giảm sản men răng có liên quan đáng kể và độc lập với ECC và S-ECC (p ≤ 0,05). KẾT LUẬN ECC rất phổ biến ở trẻ em của các gia đình mới đến ở Manitoba. Dữ liệu này sẽ cung cấp thông tin cho các nỗ lực vận động để cải thiện khả năng tiếp cận dịch vụ chăm sóc nha khoa và điều chỉnh các hoạt động thúc đẩy sức khỏe răng miệng ở trẻ em và phòng ngừa ECC cho người tị nạn và người nhập cư gần đây."} +{"idx": 1930083, "en": "BACKGROUND The need for thawed cryoprecipitate is growing. However, according to current guidelines, the shelf-life of pooled thawed cryoprecipitate at room temperature is limited because of possible bacterial contamination and loss of clotting factor activity. Here we assessed microbial growth and retention of clotting activity in cryoprecipitate stored at 4 °C after thawing to see whether its shelf life could be safely extended. MATERIALS AND METHODS Pooled thawed cryoprecipitate units (n=10) were maintained at room temperature for 6 hours and then placed at 1-6 °C for 18 hours after thawing. We examined the cryoprecipitate pools for fibrinogen, factor VIII, and von Willebrand factor activity at the following time points: 0 hours (immediately after thawing), after 6 hours at room temperature, and after 24 hours at 1-6 °C. A 5-mL aliquot from each pool was collected for aerobic and anaerobic bacterial cultures at the 24-hour time point. RESULTS Mean fibrinogen concentration and von Willebrand factor activity were similar at each time point, but factor VIII activity decreased significantly over the storage period. Bacterial growth was not detected in any cultured pooled sample. DISCUSSION Extended storing of thawed cryoprecipitate at 1-6 °C does not appear to increase the risk of bacterial contamination or affect coagulation factor activity.", "gemini": "BỐI CẢNH Nhu cầu về chế phẩm đông lạnh tan băng đang ngày càng tăng. Tuy nhiên, theo các hướng dẫn hiện tại, thời hạn sử dụng của chế phẩm đông lạnh tan băng ở nhiệt độ phòng bị hạn chế do khả năng nhiễm vi khuẩn và mất hoạt tính của yếu tố đông máu. Ở đây chúng tôi đánh giá sự phát triển của vi khuẩn và sự duy trì hoạt tính đông máu trong chế phẩm đông lạnh tan băng được bảo quản ở 4 °C sau khi rã đông để xem liệu thời hạn sử dụng của nó có thể được kéo dài một cách an toàn hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Các chế phẩm đông lạnh tan băng (n = 10) được duy trì ở nhiệt độ phòng trong 6 giờ và sau đó được đặt ở nhiệt độ 1-6 °C trong 18 giờ sau khi rã đông. Chúng tôi đã kiểm tra các chế phẩm đông lạnh tan băng về hoạt tính của fibrinogen, yếu tố VIII và yếu tố von Willebrand tại các thời điểm sau: 0 giờ (ngay sau khi rã đông), sau 6 giờ ở nhiệt độ phòng và sau 24 giờ ở nhiệt độ 1-6 °C. Một mẫu 5 mL từ mỗi chế phẩm được thu thập để nuôi cấy vi khuẩn hiếu khí và kỵ khí tại thời điểm 24 giờ. KẾT QUẢ Nồng độ fibrinogen trung bình và hoạt tính của yếu tố von Willebrand tương tự nhau tại mỗi thời điểm, nhưng hoạt tính của yếu tố VIII giảm đáng kể trong suốt thời gian bảo quản. Không phát hiện thấy sự phát triển của vi khuẩn trong bất kỳ mẫu chế phẩm đông lạnh tan băng nuôi cấy nào. THẢO LUẬN Việc bảo quản chế phẩm đông lạnh tan băng ở nhiệt độ 1-6 °C không làm tăng nguy cơ nhiễm vi khuẩn hoặc ảnh hưởng đến hoạt tính của yếu tố đông máu."} +{"idx": 1930084, "en": "BACKGROUND The safety and effectiveness of cell salvage for vaginal delivery is unknown. This case series aimed to assess the utility and adverse events related to the use of cell salvage for maternal haemorrhage during vaginal delivery. MATERIALS AND METHODS A cohort study design was chosen, focused on postpartum haemorrhages that occurred after vaginal delivery for which cell salvage equipment was requested to be set up in the labour and delivery room outside of a sterile operating room environment. Variables recorded included duration of stay in hospital, occurrence of wound infections, sepsis, thromboembolic events, and amniotic fluid embolism. RESULTS Of 28 cases of postpartum haemorrhage during vaginal deliveries involving the setup or use of cell salvage equipment, ten were associated with successful re-infusion of salvaged shed blood. These ten cases were compared to the 18 cases in which cell salvage equipment was set up, but insufficient shed blood was salvaged for re-infusion. There were no instances of postpartum sepsis, wound infection, or thromboembolism associated with the use of cell salvage for vaginal delivery. Although one case of suspected amniotic fluid embolism occurred, severe symptoms began prior to the infusion of salvaged blood. DISCUSSION Infusion of salvaged shed blood collected from a vaginal delivery field is feasible. The outcomes of these cases do not exclude an unacceptably high risk of infection or embolic events. Trials evaluating the safety and effectiveness associated with the use of cell salvage in vaginal deliveries are justified.", "gemini": "BỐI CẢNH Tính an toàn và hiệu quả của phương pháp thu hồi máu tự thân trong quá trình sinh thường vẫn chưa được biết rõ. Loạt trường hợp này nhằm đánh giá tính hữu ích và các biến cố bất lợi liên quan đến việc sử dụng phương pháp thu hồi máu tự thân để cầm máu ở sản phụ trong quá trình sinh thường. VẬT LIỆU VÀ PHƯƠNG PHÁP Đã chọn thiết kế nghiên cứu theo nhóm, tập trung vào các trường hợp băng huyết sau sinh xảy ra sau khi sinh thường, trong đó có yêu cầu thiết lập thiết bị thu hồi máu tự thân trong phòng chuyển dạ và phòng sinh bên ngoài môi trường phòng mổ vô trùng. Các biến số được ghi lại bao gồm thời gian nằm viện, tình trạng nhiễm trùng vết thương, nhiễm trùng huyết, các biến cố huyết khối tắc mạch và thuyên tắc dịch ối. KẾT QUẢ Trong số 28 trường hợp băng huyết sau sinh trong quá trình sinh thường liên quan đến việc thiết lập hoặc sử dụng thiết bị thu hồi máu tự thân, có mười trường hợp liên quan đến việc truyền lại thành công máu tự thân đã thu hồi. Mười trường hợp này được so sánh với 18 trường hợp đã thiết lập thiết bị thu hồi máu tự thân, nhưng không thu hồi được đủ máu tự thân để truyền lại. Không có trường hợp nhiễm trùng huyết sau sinh, nhiễm trùng vết thương hoặc huyết khối tắc mạch nào liên quan đến việc sử dụng phương pháp thu hồi máu tự thân trong quá trình sinh thường. Mặc dù có một trường hợp nghi ngờ thuyên tắc dịch ối, nhưng các triệu chứng nghiêm trọng đã bắt đầu trước khi truyền máu tự thân. THẢO LUẬN Việc truyền máu tự thân đã thu hồi được thu thập từ ca sinh thường là khả thi. Kết quả của những trường hợp này không loại trừ nguy cơ nhiễm trùng hoặc các biến cố thuyên tắc không thể chấp nhận được. Các thử nghiệm đánh giá tính an toàn và hiệu quả liên quan đến việc sử dụng phương pháp thu hồi máu tự thân trong quá trình sinh thường là hợp lý."} +{"idx": 1930085, "en": "Although direct oral anticoagulants (DOAC) do not require dose-adjustment on the basis of laboratory test results, the measurement of their anticoagulant effect is useful in special situations. This position paper issued by the Italian Scientific Societies that are mainly involved in the management of patients on DOAC is aimed at providing guidance to care-givers on which tests should be used and the situations in which testing is useful. The guidance is based on the data from the literature so far available and/or on consensus among experts.", "gemini": "Mặc dù thuốc chống đông đường uống trực tiếp (DOAC) không yêu cầu điều chỉnh liều dựa trên kết quả xét nghiệm, việc đo tác dụng chống đông của thuốc rất hữu ích trong những tình huống đặc biệt. Văn bản lập trường này do các Hội khoa học Ý ban hành, chủ yếu liên quan đến việc quản lý bệnh nhân dùng DOAC, nhằm mục đích cung cấp hướng dẫn cho những người chăm sóc về các xét nghiệm nên sử dụng và các tình huống mà việc xét nghiệm là hữu ích. Hướng dẫn này dựa trên dữ liệu từ các tài liệu cho đến nay và/hoặc sự đồng thuận giữa các chuyên gia."} +{"idx": 1930086, "en": "INTRODUCTION In 2014, the Canadian Task Force on Preventive Health Care (CTFPHC) recommended against routine prostate cancer screening with the prostate-specific antigen (PSA) blood test. We surveyed Canadian primary care physicians (PCPs) to understand their opinions and attitudes towards prostate cancer screening in 2016. METHODS Twenty PCPs piloted the survey to assess its accessibility. We distributed a flyer to 19 633 PCPs as an insert in a large mailed package inviting them to attend a national meeting, and later promoted the survey at the meeting. Multinomial logistic regression models examined factors associated with agreement of key guideline statements and the overall benefit of PSA screening. RESULTS A total of 1254 PCPs responded (rate of 6.4%); 54.7% of physicians aware of the CTFPHC recommendations report screening less often as a result. Overall, 55.6% of PCPs feel that the risks of PSA screening outweigh the benefits. On multivariable analysis, physicians who did not read the guidelines, did not have an academic appointment, or were in practice for over 20 years were significantly more likely to disagree with the statement that men 55-69 years old should not be screened for prostate cancer with PSA. CONCLUSIONS Our national survey found that the prostate cancer screening practices of Canadian PCPs varies widely across physician demographic groups, with almost equal numbers for or against. This has significant ethical, medical, and legal implications. The poor response rate to highly incentivized survey request may suggest a reluctance or general apathy towards this subject because of the Task Force recommendations. Future efforts should provide physicians with objective guidance around PSA screening, incorporating input from all stakeholders, including PCPs, urologists, and patients.", "gemini": "GIỚI THIỆU Năm 2014, Lực lượng đặc nhiệm về chăm sóc sức khỏe phòng ngừa Canada (CTFPHC) khuyến cáo không nên sàng lọc ung thư tuyến tiền liệt thông thường bằng xét nghiệm máu kháng nguyên đặc hiệu tuyến tiền liệt (PSA). Chúng tôi đã khảo sát các bác sĩ chăm sóc chính ở Canada (PCP) để hiểu quan điểm và thái độ của họ đối với việc sàng lọc ung thư tuyến tiền liệt vào năm 2016. PHƯƠNG PHÁP Hai mươi bác sĩ PCP đã thí điểm khảo sát để đánh giá khả năng tiếp cận của khảo sát. Chúng tôi đã phân phát một tờ rơi cho 19.633 bác sĩ PCP dưới dạng tờ rơi trong một gói thư lớn mời họ tham dự một cuộc họp toàn quốc và sau đó quảng bá cuộc khảo sát tại cuộc họp. Các mô hình hồi quy logistic đa thức đã kiểm tra các yếu tố liên quan đến sự đồng thuận về các tuyên bố hướng dẫn chính và lợi ích chung của việc sàng lọc PSA. KẾT QUẢ Tổng cộng có 1254 bác sĩ PCP đã trả lời (tỷ lệ 6,4%); 54,7% bác sĩ biết về các khuyến nghị của CTFPHC báo cáo việc sàng lọc ít thường xuyên hơn do đó. Nhìn chung, 55,6% bác sĩ PCP cảm thấy rằng những rủi ro của việc sàng lọc PSA lớn hơn những lợi ích. Theo phân tích đa biến, những bác sĩ không đọc hướng dẫn, không có cuộc hẹn học thuật hoặc hành nghề trên 20 năm có khả năng không đồng ý với tuyên bố rằng nam giới từ 55-69 tuổi không nên được sàng lọc ung thư tuyến tiền liệt bằng PSA. KẾT LUẬN Cuộc khảo sát toàn quốc của chúng tôi cho thấy rằng các hoạt động sàng lọc ung thư tuyến tiền liệt của các bác sĩ PCP Canada rất khác nhau giữa các nhóm nhân khẩu học của bác sĩ, với số lượng gần như bằng nhau ủng hộ hoặc phản đối. Điều này có ý nghĩa quan trọng về mặt đạo đức, y tế và pháp lý. Tỷ lệ phản hồi kém đối với yêu cầu khảo sát có động lực cao có thể cho thấy sự miễn cưỡng hoặc thờ ơ chung đối với chủ đề này vì các khuyến nghị của Lực lượng đặc nhiệm. Những nỗ lực trong tương lai nên cung cấp cho các bác sĩ hướng dẫn khách quan về việc sàng lọc PSA, kết hợp ý kiến đóng góp từ tất cả các bên liên quan, bao gồm các bác sĩ PCP, bác sĩ tiết niệu và bệnh nhân."} +{"idx": 1930087, "en": "INTRODUCTION Renal transplant experiences widespread success, but little is published regarding the postoperative complications. The Charlson Comorbidity Index (CCI) is a system of mortality risk assessment. Our purpose is to assess the 90-day postoperative complications after renal transplantation. The secondary objective is to clarify whether CCI predicts complications. We hypothesized increased CCI corresponds to worse complication on the Clavien scale. METHODS This is a retrospective analysis of renal recipients at our institution (2011-2013) who were ≥18 years old and received complete follow up. CCI, age, gender, body mass index (BMI), and graft type were extracted from the electronic medical records. Complications were scored using the Clavien scale. Descriptive statistics and logistic regression were used to analyze 198 patients. RESULTS The mean age was 53 (standard deviation [SD] 14), mean BMI 27.4 (SD 14), median CCI 1. Grade 2 or higher (significant) complications occurred in 60% of patients and Grade 3b or higher (severe) in 15% of patients in the 90-day postoperative period. Sixty-eight different complications were identified, the most common being blood transfusion (19%). Logistic regression suggests a predictive value of CCI (odds ratio [OR] 1.70; 95% confidence interval [CI] 1.3-2.3) for severe complications, with diabetes mellitus and peripheral vascular disease conferring increased risk. CONCLUSIONS Renal transplant carries significant risk. This data can be used to improve patient counselling on the likely postoperative course. Study limitations include the retrospective design, predisposing to potential bias in data capture.", "gemini": "NHẬP MÔN Cấy ghép thận đạt được thành công rộng rãi, nhưng có ít tài liệu nào được công bố về các biến chứng sau phẫu thuật. Chỉ số đồng mắc bệnh Charlson (CCI) là một hệ thống đánh giá rủi ro tử vong. Mục đích của chúng tôi là đánh giá các biến chứng sau phẫu thuật trong vòng 90 ngày sau khi ghép thận. Mục tiêu thứ cấp là làm rõ CCI có dự đoán được biến chứng hay không. Chúng tôi đưa ra giả thuyết rằng CCI tăng tương ứng với biến chứng tệ hơn trên thang Clavien. PHƯƠNG PHÁP Đây là một phân tích hồi cứu đối với những người nhận thận tại cơ sở của chúng tôi (2011-2013) từ 18 tuổi trở lên và được theo dõi đầy đủ. CCI, tuổi, giới tính, chỉ số khối cơ thể (BMI) và loại ghép được trích xuất từ hồ sơ bệnh án điện tử. Các biến chứng được chấm điểm theo thang Clavien. Thống kê mô tả và hồi quy logistic được sử dụng để phân tích 198 bệnh nhân. KẾT QUẢ Độ tuổi trung bình là 53 (độ lệch chuẩn [SD] 14), BMI trung bình 27,4 (SD 14), CCI trung bình 1. Biến chứng độ 2 trở lên (nặng) xảy ra ở 60% bệnh nhân và độ 3b trở lên (nghiêm trọng) ở 15% bệnh nhân trong giai đoạn hậu phẫu 90 ngày. Sáu mươi tám biến chứng khác nhau đã được xác định, phổ biến nhất là truyền máu (19%). Hồi quy logistic cho thấy giá trị dự đoán của CCI (tỷ lệ cược [OR] 1,70; khoảng tin cậy 95% [CI] 1,3-2,3) đối với các biến chứng nghiêm trọng, trong đó đái tháo đường và bệnh mạch máu ngoại vi làm tăng nguy cơ. KẾT LUẬN Ghép thận có nguy cơ đáng kể. Dữ liệu này có thể được sử dụng để cải thiện việc tư vấn cho bệnh nhân về quá trình hậu phẫu có thể xảy ra. Hạn chế của nghiên cứu bao gồm thiết kế hồi cứu, dẫn đến khả năng thiên lệch trong việc thu thập dữ liệu."} +{"idx": 1930088, "en": "Only a few nutritional factors have been identified to predict the risk of developing complications after radical cystectomy (RC). This narrative review delineates the current known effects of preoperative nutritional status factors in this context. The report highlights the heterogeneity between study methods and results. We determined that low albuminemia values increase mortality risk and overall complications. In addition, obesity tends to increase the risk of developing venous thromboembolism and adverse events. Additional prospective studies, using standardized methods to both define and report complications, should be conducted to strengthen the connections between preoperative nutritional status factors and post-RC complications. Furthermore, intervention studies testing the impact of strategies to improve nutritional status on the risk of complications after RC are also needed.", "gemini": "Chỉ một vài yếu tố dinh dưỡng được xác định là có thể dự đoán nguy cơ phát triển các biến chứng sau phẫu thuật cắt bỏ bàng quang tận gốc (RC). Đánh giá tường thuật này mô tả chi tiết các tác động đã biết hiện nay của các yếu tố tình trạng dinh dưỡng trước phẫu thuật trong bối cảnh này. Báo cáo nêu bật tính không đồng nhất giữa các phương pháp nghiên cứu và kết quả. Chúng tôi xác định rằng giá trị albumin huyết thấp làm tăng nguy cơ tử vong và biến chứng nói chung. Ngoài ra, béo phì có xu hướng làm tăng nguy cơ mắc huyết khối tĩnh mạch và các biến cố bất lợi. Cần tiến hành thêm các nghiên cứu triển vọng, sử dụng các phương pháp chuẩn hóa để xác định và báo cáo các biến chứng, nhằm củng cố mối liên hệ giữa các yếu tố tình trạng dinh dưỡng trước phẫu thuật và các biến chứng sau RC. Hơn nữa, cũng cần các nghiên cứu can thiệp để kiểm tra tác động của các chiến lược cải thiện tình trạng dinh dưỡng đối với nguy cơ biến chứng sau RC."} +{"idx": 1930089, "en": "It is critically important to define disease-specific research priorities to better allocate limited resources. There is growing recognition of the value of involving patients and caregivers, as well as expert clinicians in this process. To our knowledge, this has not been done this way for kidney cancer. Using the transparent and inclusive process established by the James Lind Alliance, the Kidney Cancer Research Network of Canada (KCRNC) sponsored a collaborative consensus-based priority-setting partnership (PSP) to identify research priorities in the management of kidney cancer. The final result was identification of 10 research priorities for kidney cancer, which are discussed in the context of current initiatives and gaps in knowledge. This process provided a systematic and effective way to collaboratively establish research priorities with patients, caregivers, and clinicians, and provides a valuable resource for researchers and funding agencies.", "gemini": "Việc xác định các ưu tiên nghiên cứu cụ thể cho từng bệnh là rất quan trọng để phân bổ tốt hơn các nguồn lực hạn chế. Giá trị của việc có sự tham gia của bệnh nhân và người chăm sóc, cũng như các bác sĩ lâm sàng chuyên gia trong quá trình này ngày càng được công nhận. Theo hiểu biết của chúng tôi, điều này chưa được thực hiện theo cách này đối với ung thư thận. Sử dụng quy trình minh bạch và toàn diện do Liên minh James Lind thiết lập, Mạng lưới nghiên cứu ung thư thận Canada (KCRNC) đã tài trợ cho một quan hệ đối tác thiết lập ưu tiên dựa trên sự đồng thuận hợp tác (PSP) để xác định các ưu tiên nghiên cứu trong việc quản lý ung thư thận. Kết quả cuối cùng là xác định được 10 ưu tiên nghiên cứu đối với ung thư thận, được thảo luận trong bối cảnh các sáng kiến hiện tại và những lỗ hổng trong kiến thức. Quy trình này cung cấp một cách có hệ thống và hiệu quả để thiết lập các ưu tiên nghiên cứu một cách hợp tác với bệnh nhân, người chăm sóc và bác sĩ lâm sàng, đồng thời cung cấp một nguồn lực có giá trị cho các nhà nghiên cứu và các cơ quan tài trợ."} +{"idx": 1930090, "en": "INTRODUCTION This study aims to empirically validate the French-Canadian version of the Expanded Prostate Cancer Index Composite (EPIC), a measure of health-related quality of life for prostate cancer patients. METHODS Two hundred fifty-one participants completed a battery of self-report scales, including the French-Canadian version of the EPIC, after having received radiation therapy or radical prostatectomy for prostate cancer. RESULTS The internal consistency for the urinary incontinence, bowel, and sexual domains of the EPIC-26 was high (Cronbach's alpha coefficients from 0.80-0.92), while coefficients for the urinary irritation/obstruction (0.59) and hormonal (0.67) domains were lower. Item-total correlations (rs=0.15-0.85), and temporal stability (rs=0.72-0.93) generally supported the reliability of the instrument. The five-factor structure of the EPIC-26 was confirmed for the most part. The construct validity of the instrument was also supported by high correlations obtained between each domain and measures assessing similar constructs (rs=-0.56-0.83). The EPIC also showed an excellent sensitivity to change with significant differences obtained on EPIC scores (all p<0.05) between pre- and post-prostate cancer treatment. CONCLUSIONS The psychometric qualities of the French-Canadian version of the EPIC are well-supported, thus providing a valid tool to assess health-related quality of life in prostate cancer patients.", "gemini": "GIỚI THIỆU Nghiên cứu này nhằm mục đích xác nhận giá trị thực nghiệm của phiên bản tiếng Pháp-Canada của Chỉ số Ung thư tuyến tiền liệt mở rộng tổng hợp (EPIC), một biện pháp về chất lượng cuộc sống liên quan đến sức khỏe của bệnh nhân ung thư tuyến tiền liệt. PHƯƠNG PHÁP Hai trăm năm mươi mốt người tham gia đã hoàn thành một loạt các thang tự báo cáo, bao gồm phiên bản tiếng Pháp-Canada của EPIC, sau khi được xạ trị hoặc phẫu thuật cắt tuyến tiền liệt triệt để để điều trị ung thư tuyến tiền liệt. KẾT QUẢ Độ tin cậy nội bộ đối với các lĩnh vực tiểu không tự chủ, đại tiện và tình dục của EPIC-26 là cao (các hệ số alpha của Cronbach từ 0,80-0,92), trong khi các hệ số cho các lĩnh vực kích ứng / tắc nghẽn đường tiết niệu (0,59) và nội tiết tố (0,67) là thấp hơn. Các tương quan tổng số mục (rs = 0,15-0,85) và độ ổn định theo thời gian (rs = 0,72-0,93) thường hỗ trợ độ tin cậy của công cụ. Cấu trúc năm yếu tố của EPIC-26 đã được xác nhận phần lớn. Tính hợp lệ của cấu trúc của công cụ cũng được hỗ trợ bởi các tương quan cao thu được giữa mỗi lĩnh vực và các biện pháp đánh giá các cấu trúc tương tự (rs = -0,56-0,83). EPIC cũng cho thấy độ nhạy tuyệt vời để thay đổi với các sự khác biệt đáng kể thu được trên điểm EPIC (tất cả p <0,05) giữa trước và sau khi điều trị ung thư tuyến tiền liệt. KẾT LUẬN Các phẩm chất trắc nghiệm tâm lý của phiên bản tiếng Pháp-Canada của EPIC được hỗ trợ tốt, do đó cung cấp một công cụ hợp lệ để đánh giá chất lượng cuộc sống liên quan đến sức khỏe ở bệnh nhân ung thư tuyến tiền liệt."} +{"idx": 1930091, "en": "INTRODUCTION Telephone consultations are part of a strategy to allow access to medical expertise. Telephone consultations have been fee-for-services benefits in the province of Quebec since 2012. Recent studies have shown that adequate communication is one of the most common means to prevent disability and death. We sought to determine the characteristics of phone consultations made to a tertiary centre's urologists and to characterize their experience. METHODS We performed a prospective study using all billing receipts filed by 15 academic urologists for phone consultations received during a 10-month period. A descriptive analysis was done to collect the principal characteristics of all phone calls received. Moreover, an online survey was distributed to those urologists. The survey was composed of 10 multiple-choice questions to review their personal experience. RESULTS A total of 678 billing receipts were analyzed. The most common reasons for calls were lithiasis (11.5%), hematuria (10.5%), and urinary retention (8.4%). Most phone calls (57.7%) were made by emergency physicians and family doctors. The majority (88.7%) of calls were placed between 8:00 am and 5:00 pm. Most of the calls came from the immediate region covered by the group. Our survey demonstrated that urologists pay more attention to document telephone consultations since the introduction of the new remuneration plan. Most urologists found the phone consultations to be relevant. CONCLUSIONS Lithiasis and hematuria are the primary reasons for telephone consultations. Continuing medical education on these subjects could be worthwhile. The RAMQ remuneration plan has improved documentation of phone consultations by urologists.", "gemini": "GIỚI THIỆU Tư vấn qua điện thoại là một phần của chiến lược cho phép tiếp cận với chuyên môn y tế. Tư vấn qua điện thoại đã được hưởng lợi ích thanh toán cho dịch vụ tại tỉnh Quebec kể từ năm 2012. Các nghiên cứu gần đây đã chỉ ra rằng giao tiếp đầy đủ là một trong những phương tiện phổ biến nhất để ngăn ngừa tình trạng tàn tật và tử vong. Chúng tôi tìm cách xác định các đặc điểm của các cuộc tư vấn qua điện thoại được thực hiện cho các bác sĩ tiết niệu của một trung tâm chuyên khoa và mô tả đặc điểm kinh nghiệm của họ. PHƯƠNG PHÁP Chúng tôi đã thực hiện một nghiên cứu triển vọng sử dụng tất cả các biên lai thanh toán do 15 bác sĩ tiết niệu học nộp cho các cuộc tư vấn qua điện thoại được nhận trong khoảng thời gian 10 tháng. Một phân tích mô tả đã được thực hiện để thu thập các đặc điểm chính của tất cả các cuộc gọi điện thoại nhận được. Hơn nữa, một cuộc khảo sát trực tuyến đã được phân phối cho những bác sĩ tiết niệu đó. Cuộc khảo sát bao gồm 10 câu hỏi trắc nghiệm để xem xét kinh nghiệm cá nhân của họ. KẾT QUẢ Tổng cộng có 678 biên lai thanh toán đã được phân tích. Các lý do phổ biến nhất để gọi điện là sỏi (11,5%), tiểu ra máu (10,5%) và bí tiểu (8,4%). Hầu hết các cuộc gọi điện thoại (57,7%) được thực hiện bởi các bác sĩ cấp cứu và bác sĩ gia đình. Phần lớn (88,7%) các cuộc gọi được thực hiện từ 8:00 sáng đến 5:00 chiều. Hầu hết các cuộc gọi đến từ khu vực lân cận do nhóm phụ trách. Cuộc khảo sát của chúng tôi chứng minh rằng các bác sĩ tiết niệu chú ý nhiều hơn đến việc lập hồ sơ tư vấn qua điện thoại kể từ khi có kế hoạch trả lương mới. Hầu hết các bác sĩ tiết niệu đều thấy tư vấn qua điện thoại có liên quan. KẾT LUẬN Sỏi và tiểu ra máu là những lý do chính để tư vấn qua điện thoại. Giáo dục y tế liên tục về các chủ đề này có thể là điều đáng giá. Kế hoạch trả lương của RAMQ đã cải thiện việc lập hồ sơ tư vấn qua điện thoại của các bác sĩ tiết niệu."} +{"idx": 1930092, "en": "The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order in human cells using fluorescence polarization microscopy and found that cleavage furrow ingression initiates by contraction of an equatorial actin network with randomly oriented filaments. The network subsequently gradually reoriented actin filaments along the cell equator. This strictly depended on myosin II activity, suggesting local network reorganization by mechanical forces. Cortical laser microsurgery revealed that during cytokinesis progression, mechanical tension increased substantially along the direction of the cell equator, while the network contracted laterally along the pole-to-pole axis without a detectable increase in tension. Our data suggest that an asymmetric increase in cortical tension promotes filament reorientation along the cytokinetic cleavage furrow, which might have implications for diverse other biological processes involving actomyosin rings.", "gemini": "Vòng actomyosin tạo ra lực để xâm nhập vào rãnh phân cắt động học trong các tế bào động vật, nhưng tổ chức sợi và cơ chế co bóp của nó vẫn chưa được hiểu rõ. Chúng tôi định lượng trật tự sợi actin trong các tế bào người bằng kính hiển vi phân cực huỳnh quang và phát hiện ra rằng sự xâm nhập của rãnh phân cắt bắt đầu bằng sự co bóp của một mạng lưới actin xích đạo với các sợi định hướng ngẫu nhiên. Sau đó, mạng lưới dần dần định hướng lại các sợi actin dọc theo đường xích đạo của tế bào. Điều này hoàn toàn phụ thuộc vào hoạt động của myosin II, cho thấy sự sắp xếp lại mạng lưới cục bộ do các lực cơ học. Phẫu thuật vi phẫu bằng tia laser ở vỏ não cho thấy trong quá trình tiến triển của động học, sức căng cơ học tăng đáng kể theo hướng của đường xích đạo của tế bào, trong khi mạng lưới co lại theo chiều ngang dọc theo trục cực-cực mà không có sự gia tăng sức căng nào có thể phát hiện được. Dữ liệu của chúng tôi cho thấy sự gia tăng không đối xứng của sức căng ở vỏ não thúc đẩy sự định hướng lại của sợi dọc theo rãnh phân cắt động học, điều này có thể có ý nghĩa đối với nhiều quá trình sinh học khác liên quan đến vòng actomyosin."} +{"idx": 1930093, "en": "The entry of calcium into mitochondria is central to metabolism, inter-organelle communication, and cell life/death decisions. Long-sought transporters involved in mitochondrial calcium influx and efflux have recently been identified. To obtain a unified picture of mitochondrial calcium utilization, a parallel advance in understanding the forms and quantities of mitochondrial calcium stores is needed. We present here the direct 3D visualization of mitochondrial calcium in intact mammalian cells using cryo-scanning transmission electron tomography (CSTET). Amorphous solid granules containing calcium and phosphorus were pervasive in the mitochondrial matrices of a variety of mammalian cell types. Analysis based on quantitative electron scattering revealed that these repositories are equivalent to molar concentrations of dissolved ions. These results demonstrate conclusively that calcium buffering in the mitochondrial matrix in live cells occurs by phase separation, and that solid-phase stores provide a major ion reservoir that can be mobilized for bioenergetics and signaling.", "gemini": "Sự xâm nhập của canxi vào ty thể là trung tâm của quá trình trao đổi chất, liên lạc giữa các bào quan và các quyết định về sự sống/chết của tế bào. Các chất vận chuyển từ lâu được tìm kiếm liên quan đến dòng vào và dòng ra canxi của ty thể đã được xác định gần đây. Để có được một bức tranh thống nhất về việc sử dụng canxi của ty thể, cần có một tiến bộ song song trong việc hiểu các dạng và số lượng của các kho dự trữ canxi của ty thể. Chúng tôi trình bày ở đây hình ảnh trực quan 3D trực tiếp về canxi ty thể trong các tế bào động vật có vú còn nguyên vẹn bằng cách sử dụng chụp cắt lớp điện tử truyền qua quét đông lạnh (CSTET). Các hạt rắn vô định hình chứa canxi và phốt pho có ở khắp nơi trong các ma trận ty thể của nhiều loại tế bào động vật có vú. Phân tích dựa trên sự tán xạ electron định lượng cho thấy rằng các kho chứa này tương đương với nồng độ mol của các ion hòa tan. Những kết quả này chứng minh một cách thuyết phục rằng sự đệm canxi trong ma trận ty thể trong các tế bào sống xảy ra thông qua sự tách pha và các kho chứa pha rắn cung cấp một bể chứa ion lớn có thể được huy động để tạo năng lượng sinh học và truyền tín hiệu."} +{"idx": 1930094, "en": "The cellular pathways that restart stalled replication forks are essential for genome stability and tumor prevention. However, how many of these pathways exist in cells and how these pathways are selectively activated remain unclear. Here, we describe two major fork restart pathways, and demonstrate that their selection is governed by 53BP1 and BRCA1, which are known to control the pathway choice to repair double-strand DNA breaks (DSBs). Specifically, 53BP1 promotes a fork cleavage-free pathway, whereas BRCA1 facilitates a break-induced replication (BIR) pathway coupled with SLX-MUS complex-mediated fork cleavage. The defect in the first pathway, but not DSB repair, in a 53BP1 mutant is largely corrected by disrupting BRCA1, and vice versa. Moreover, PLK1 temporally regulates the switch of these two pathways through enhancing the assembly of the SLX-MUS complex. Our results reveal two distinct fork restart pathways, which are antagonistically controlled by 53BP1 and BRCA1 in a DSB repair-independent manner.", "gemini": "Các con đường tế bào khởi động lại các ngã ba sao chép bị đình trệ là rất cần thiết cho sự ổn định của bộ gen và ngăn ngừa khối u. Tuy nhiên, vẫn chưa rõ có bao nhiêu con đường này tồn tại trong các tế bào và cách các con đường này được kích hoạt có chọn lọc. Ở đây, chúng tôi mô tả hai con đường khởi động lại ngã ba chính và chứng minh rằng sự lựa chọn của chúng được điều khiển bởi 53BP1 và BRCA1, được biết là kiểm soát sự lựa chọn con đường để sửa chữa các vết đứt gãy DNA sợi đôi (DSB). Cụ thể, 53BP1 thúc đẩy một con đường không có cắt ngã ba, trong khi BRCA1 tạo điều kiện cho một con đường sao chép gây ra đứt gãy (BIR) kết hợp với cắt ngã ba do phức hợp SLX-MUS trung gian. Khiếm khuyết ở con đường đầu tiên, nhưng không phải sửa chữa DSB, ở một đột biến 53BP1 phần lớn được sửa chữa bằng cách phá vỡ BRCA1, và ngược lại. Hơn nữa, PLK1 điều chỉnh tạm thời sự chuyển đổi của hai con đường này thông qua việc tăng cường lắp ráp phức hợp SLX-MUS. Kết quả của chúng tôi cho thấy hai con đường khởi động lại ngã ba riêng biệt, được kiểm soát đối kháng bởi 53BP1 và BRCA1 theo cách độc lập với sửa chữa DSB."} +{"idx": 1930095, "en": "High-resolution daylight vision is mediated by cone photoreceptors. The molecular program responsible for the formation of their light sensor, the outer segment, is not well understood. We correlated daily changes in ultrastructure and gene expression in postmitotic mouse cones, between birth and eye opening, using serial block-face electron microscopy (EM) and RNA sequencing. Outer segments appeared rapidly at postnatal day six and their appearance coincided with a switch in gene expression. The switch affected over 14% of all expressed genes. Genes that switched off were rich in transcription factors and neurogenic genes. Those that switched on contained genes relevant for cone function. Chromatin rearrangements in enhancer regions occurred before the switch was completed, but not after. We provide a resource comprised of correlated EM, RNAseq, and ATACseq data, showing that the growth of a key compartment of a postmitotic cell involves an extensive switch in gene expression and chromatin accessibility.", "gemini": "Tầm nhìn ban ngày có độ phân giải cao được trung gian bởi các thụ thể hình nón. Chương trình phân tử chịu trách nhiệm hình thành cảm biến ánh sáng của chúng, đoạn ngoài, vẫn chưa được hiểu rõ. Chúng tôi đã tương quan những thay đổi hàng ngày về cấu trúc siêu vi và biểu hiện gen ở các tế bào hình nón chuột sau nguyên phân, giữa thời điểm sinh và mở mắt, bằng cách sử dụng kính hiển vi điện tử quét khối nối tiếp (EM) và giải trình tự RNA. Các đoạn ngoài xuất hiện nhanh chóng vào ngày thứ sáu sau sinh và sự xuất hiện của chúng trùng với sự thay đổi trong biểu hiện gen. Sự thay đổi ảnh hưởng đến hơn 14% tất cả các gen được biểu hiện. Các gen bị tắt rất giàu yếu tố phiên mã và gen thần kinh. Những gen được bật có chứa các gen có liên quan đến chức năng hình nón. Sự sắp xếp lại chromatin ở các vùng tăng cường đã xảy ra trước khi sự thay đổi hoàn tất, nhưng không phải sau đó. Chúng tôi cung cấp một nguồn tài nguyên bao gồm dữ liệu EM, RNAseq và ATACseq có tương quan, cho thấy sự phát triển của một khoang chính của tế bào sau nguyên phân liên quan đến sự thay đổi rộng rãi trong biểu hiện gen và khả năng tiếp cận chromatin."} +{"idx": 1930096, "en": "Beta oscillations (15-29Hz) are among the most prominent signatures of brain activity. Beta power is predictive of healthy and abnormal behaviors, including perception, attention and motor action. In non-averaged signals, beta can emerge as transient high-power 'events'. As such, functionally relevant differences in averaged power across time and trials can reflect changes in event number, power, duration, and/or frequency span. We show that functionally relevant differences in averaged beta power in primary somatosensory neocortex reflect a difference in the number of high-power beta events per trial, i.e. event rate. Further, beta events occurring close to the stimulus were more likely to impair perception. These results are consistent across detection and attention tasks in human magnetoencephalography, and in local field potentials from mice performing a detection task. These results imply that an increased propensity of beta events predicts the failure to effectively transmit information through specific neocortical representations.", "gemini": "Dao động beta (15-29Hz) là một trong những đặc điểm nổi bật nhất của hoạt động não. Sức mạnh beta có thể dự đoán các hành vi lành mạnh và bất thường, bao gồm nhận thức, chú ý và hành động vận động. Trong các tín hiệu không trung bình, beta có thể xuất hiện như các 'sự kiện' công suất cao thoáng qua. Do đó, sự khác biệt có liên quan về mặt chức năng trong công suất trung bình theo thời gian và các thử nghiệm có thể phản ánh những thay đổi về số lượng sự kiện, công suất, thời lượng và/hoặc khoảng tần số. Chúng tôi chỉ ra rằng sự khác biệt có liên quan về mặt chức năng trong công suất beta trung bình ở vỏ não somatosensory chính phản ánh sự khác biệt về số lượng các sự kiện beta công suất cao trên mỗi thử nghiệm, tức là tỷ lệ sự kiện. Hơn nữa, các sự kiện beta xảy ra gần kích thích có nhiều khả năng làm suy yếu nhận thức. Những kết quả này phù hợp trên các nhiệm vụ phát hiện và chú ý trong từ não đồ của con người và trong các điện thế trường cục bộ từ những con chuột thực hiện nhiệm vụ phát hiện. Những kết quả này ngụ ý rằng khả năng xảy ra nhiều hơn của các sự kiện beta dự đoán sự thất bại trong việc truyền thông tin hiệu quả thông qua các biểu diễn vỏ não cụ thể."} +{"idx": 1930097, "en": "Sleep is an essential and phylogenetically conserved behavioral state, but it remains unclear to what extent genes identified in invertebrates also regulate vertebrate sleep. RFamide-related neuropeptides have been shown to promote invertebrate sleep, and here we report that the vertebrate hypothalamic RFamide neuropeptide VF (NPVF) regulates sleep in the zebrafish, a diurnal vertebrate. We found that NPVF signaling and npvf-expressing neurons are both necessary and sufficient to promote sleep, that mature peptides derived from the NPVF preproprotein promote sleep in a synergistic manner, and that stimulation of npvf-expressing neurons induces neuronal activity levels consistent with normal sleep. These results identify NPVF signaling and npvf-expressing neurons as a novel vertebrate sleep-promoting system and suggest that RFamide neuropeptides participate in an ancient and central aspect of sleep control.", "gemini": "Giấc ngủ là một trạng thái hành vi thiết yếu và được bảo tồn theo kiểu phát sinh loài, nhưng vẫn chưa rõ ở mức độ nào các gen được xác định ở động vật không xương sống cũng điều chỉnh giấc ngủ của động vật có xương sống. Các neuropeptide liên quan đến RFamide đã được chứng minh là thúc đẩy giấc ngủ của động vật không xương sống, và ở đây chúng tôi báo cáo rằng neuropeptide RFamide vùng dưới đồi của động vật có xương sống VF (NPVF) điều chỉnh giấc ngủ ở cá ngựa vằn, một loài động vật có xương sống hoạt động vào ban ngày. Chúng tôi phát hiện ra rằng tín hiệu NPVF và các tế bào thần kinh biểu hiện npvf vừa cần thiết vừa đủ để thúc đẩy giấc ngủ, các peptide trưởng thành có nguồn gốc từ tiền protein NPVF thúc đẩy giấc ngủ theo cách hiệp đồng, và sự kích thích các tế bào thần kinh biểu hiện npvf gây ra mức độ hoạt động của tế bào thần kinh phù hợp với giấc ngủ bình thường. Những kết quả này xác định tín hiệu NPVF và các tế bào thần kinh biểu hiện npvf là một hệ thống thúc đẩy giấc ngủ mới của động vật có xương sống và cho thấy rằng các neuropeptide RFamide tham gia vào một khía cạnh cổ xưa và trung tâm của quá trình kiểm soát giấc ngủ."} +{"idx": 1930098, "en": "Direct use of the total scatter factor (S tot) for independent monitor unit (MU) calculations can be a good alternative approach to the traditional separate treatment of head/collimator scatter (S c) and phantom scatter (S p), especially for stereotactic small fields under the simultaneous collimation of secondary jaws and tertiary multileaf collimators (MLC). We have carried out the measurement of S tot in water for field sizes down to 0.5  ×  0.5 cm2 on a Varian TrueBeam STx medical linear accelerator (linac) equipped with high definition MLCs. Both the jaw field size (c) and MLC field size (s) significantly impact the linac output factors, especially when c [Formula: see text] s and s is small (e.g. s  <  5 cm). The combined influence of MLC and jaws gives rise to a two-argument dependence of the total scatter factor, S tot(c,s), which is difficult to functionally decouple. The (c,s) dependence can be conceived as a set of s-dependent functions ('branches') defined on domain [s min, s max  =  c] for a given jaw size of c. We have also developed a heuristic model of S tot to assist the clinical implementation of the measured S tot data for small field dosimetry. The model has two components: (i) empirical fit formula for the s-dependent branches and (ii) interpolation scheme between the branches. The interpolation scheme preserves the characteristic shape of the measured branches and effectively transforms the measured trapezoidal domain in (c,s) plane to a rectangular domain to facilitate easier two-dimensional interpolation to determine S tot for arbitrary (c,s) combinations. Both the empirical fit and interpolation showed good agreement with experimental validation data.", "gemini": "Việc sử dụng trực tiếp hệ số tán xạ tổng (S tot) cho các phép tính đơn vị giám sát (MU) độc lập có thể là một cách tiếp cận thay thế tốt cho cách xử lý riêng biệt truyền thống đối với tán xạ đầu/ống chuẩn trực (S c) và tán xạ bóng ma (S p), đặc biệt đối với các trường nhỏ lập thể dưới sự chuẩn trực đồng thời của hàm thứ cấp và chuẩn trực đa lá thứ cấp (MLC). Chúng tôi đã thực hiện phép đo S tot trong nước đối với kích thước trường xuống đến 0,5 × 0,5 cm2 trên máy gia tốc tuyến tính y tế Varian TrueBeam STx (linac) được trang bị MLC độ nét cao. Cả kích thước trường hàm (c) và kích thước trường MLC (s) đều ảnh hưởng đáng kể đến các hệ số đầu ra của linac, đặc biệt khi c [Công thức: xem văn bản] s và s nhỏ (ví dụ: s < 5 cm). Ảnh hưởng kết hợp của MLC và hàm làm phát sinh sự phụ thuộc hai đối số của hệ số tán xạ tổng, S tot(c,s), rất khó tách rời về mặt chức năng. Sự phụ thuộc (c,s) có thể được hình thành như một tập hợp các hàm phụ thuộc vào s ('các nhánh') được xác định trên miền [s min, s max = c] cho một kích thước hàm nhất định của c. Chúng tôi cũng đã phát triển một mô hình thực nghiệm của S tot để hỗ trợ việc triển khai lâm sàng dữ liệu S tot đã đo được cho phép đo liều trường nhỏ. Mô hình có hai thành phần: (i) công thức phù hợp theo kinh nghiệm cho các nhánh phụ thuộc vào s và (ii) lược đồ nội suy giữa các nhánh. Lược đồ nội suy bảo toàn hình dạng đặc trưng của các nhánh đã đo được và chuyển đổi hiệu quả miền hình thang đã đo được trong mặt phẳng (c,s) thành miền hình chữ nhật để tạo điều kiện nội suy hai chiều dễ dàng hơn để xác định S tot cho các tổ hợp (c,s) tùy ý. Cả phép phù hợp theo kinh nghiệm và phép nội suy đều cho thấy sự phù hợp tốt với dữ liệu xác thực thực nghiệm."} +{"idx": 1930099, "en": "Photosensitizer fluorescence excited by photodynamic therapy (PDT) treatment light can be used to monitor the in vivo concentration of the photosensitizer and its photobleaching. The temporal integral of the product of in vivo photosensitizer concentration and light fluence is called PDT dose, which is an important dosimetry quantity for PDT. However, the detected photosensitizer fluorescence may be distorted by variations in the absorption and scattering of both excitation and fluorescence light in tissue. Therefore, correction of the measured fluorescence for distortion due to variable optical properties is required for absolute quantification of photosensitizer concentration. In this study, we have developed a four-channel PDT dose dosimetry system to simultaneously acquire light dosimetry and photosensitizer fluorescence data. We measured PDT dose at four sites in the pleural cavity during pleural PDT. We have determined an empirical optical property correction function using Monte Carlo simulations of fluorescence for a range of physiologically relevant tissue optical properties. Parameters of the optical property correction function for Photofrin fluorescence were determined experimentally using tissue-simulating phantoms. In vivo measurements of photosensitizer fluorescence showed negligible photobleaching of Photofrin during the PDT treatment, but large intra- and inter-patient heterogeneities of in vivo Photofrin concentration are observed. PDT doses delivered to 22 sites in the pleural cavity of 8 patients were different by 2.9 times intra-patient and 8.3 times inter-patient.", "gemini": "Huỳnh quang chất cảm quang được kích thích bởi ánh sáng điều trị liệu pháp quang động lực (PDT) có thể được sử dụng để theo dõi nồng độ chất cảm quang trong cơ thể sống và sự tẩy trắng quang học của nó. Tích phân thời gian của tích số nồng độ chất cảm quang trong cơ thể sống và mật độ dòng sáng được gọi là liều PDT, là một đại lượng định lượng liều quan trọng đối với PDT. Tuy nhiên, huỳnh quang chất cảm quang được phát hiện có thể bị bóp méo do sự thay đổi trong sự hấp thụ và tán xạ của cả ánh sáng kích thích và huỳnh quang trong mô. Do đó, cần phải hiệu chỉnh huỳnh quang được đo để khắc phục sự bóp méo do các đặc tính quang học thay đổi để định lượng tuyệt đối nồng độ chất cảm quang. Trong nghiên cứu này, chúng tôi đã phát triển một hệ thống định lượng liều PDT bốn kênh để đồng thời thu thập dữ liệu định lượng liều ánh sáng và huỳnh quang chất cảm quang. Chúng tôi đã đo liều PDT tại bốn vị trí trong khoang màng phổi trong quá trình PDT màng phổi. Chúng tôi đã xác định một hàm hiệu chỉnh đặc tính quang học kinh nghiệm bằng cách sử dụng mô phỏng Monte Carlo về huỳnh quang cho một loạt các đặc tính quang học của mô có liên quan đến sinh lý. Các thông số của hàm hiệu chỉnh đặc tính quang học đối với huỳnh quang Photofrin đã được xác định bằng thực nghiệm bằng cách sử dụng các mô hình mô phỏng mô. Các phép đo trong cơ thể sống về huỳnh quang chất cảm quang cho thấy sự tẩy trắng quang học không đáng kể của Photofrin trong quá trình điều trị PDT, nhưng quan sát thấy sự không đồng nhất lớn trong cơ thể và giữa các bệnh nhân về nồng độ Photofrin trong cơ thể sống. Liều PDT được truyền đến 22 vị trí trong khoang màng phổi của 8 bệnh nhân khác nhau gấp 2,9 lần trong bệnh nhân và 8,3 lần giữa các bệnh nhân."} +{"idx": 1930100, "en": "Primary immunodeficiencies are often monogenic disorders characterized by vulnerability to specific infectious pathogens. Here, we performed whole-exome sequencing of a patient with disseminated Mycobacterium abscessus, Streptococcus viridians bacteremia, and cytomegalovirus (CMV) viremia and identified mutations in 2 genes that regulate distinct IFN pathways. The patient had a homozygous frameshift deletion in IFNGR2, which encodes the signal transducing chain of the IFN-γ receptor, that resulted in minimal protein expression and abolished downstream signaling. The patient also harbored a homozygous deletion in IFNAR1 (IFNAR1*557Gluext*46), which encodes the IFN-α receptor signaling subunit. The IFNAR1*557Gluext*46 resulted in replacement of the stop codon with 46 additional codons at the C-terminus. The level of IFNAR1*557Gluext*46 mutant protein expressed in patient fibroblasts was comparable to levels of WT IFNAR1 in control fibroblasts. IFN-α-induced signaling was impaired in the patient fibroblasts, as evidenced by decreased STAT1/STAT2 phosphorylation, nuclear translocation of STAT1, and expression of IFN-α-stimulated genes critical for CMV immunity. Pretreatment with IFN-α failed to suppress CMV protein expression in patient fibroblasts, whereas expression of WT IFNAR1 restored IFN-α-mediated suppression of CMV. This study identifies a human IFNAR1 mutation and describes a digenic immunodeficiency specific to type I and type II IFNs.", "gemini": "Các tình trạng suy giảm miễn dịch nguyên phát thường là các rối loạn đơn gen đặc trưng bởi tính dễ bị tổn thương với các tác nhân gây bệnh truyền nhiễm cụ thể. Ở đây, chúng tôi đã thực hiện giải trình tự toàn bộ exome của một bệnh nhân bị Mycobacterium abscessus lan tỏa, nhiễm khuẩn huyết Streptococcus viridians và nhiễm virus Cytomegalovirus (CMV) và xác định các đột biến trong 2 gen điều chỉnh các con đường IFN riêng biệt. Bệnh nhân có một đột biến dịch khung đồng hợp tử ở IFNGR2, mã hóa chuỗi truyền tín hiệu của thụ thể IFN-γ, dẫn đến biểu hiện protein tối thiểu và xóa tín hiệu hạ lưu. Bệnh nhân cũng mang một đột biến xóa đồng hợp tử ở IFNAR1 (IFNAR1*557Gluext*46), mã hóa tiểu đơn vị tín hiệu của thụ thể IFN-α. IFNAR1*557Gluext*46 dẫn đến việc thay thế codon dừng bằng 46 codon bổ sung ở đầu C. Mức protein đột biến IFNAR1*557Gluext*46 được biểu hiện trong nguyên bào sợi của bệnh nhân tương đương với mức WT IFNAR1 trong nguyên bào sợi đối chứng. Tín hiệu do IFN-α gây ra bị suy yếu trong nguyên bào sợi của bệnh nhân, bằng chứng là sự phosphoryl hóa STAT1/STAT2 giảm, sự chuyển vị hạt nhân của STAT1 và sự biểu hiện của các gen kích thích IFN-α rất quan trọng đối với khả năng miễn dịch CMV. Việc tiền xử lý bằng IFN-α không ức chế được biểu hiện protein CMV trong nguyên bào sợi của bệnh nhân, trong khi biểu hiện của WT IFNAR1 khôi phục lại sự ức chế trung gian IFN-α đối với CMV. Nghiên cứu này xác định một đột biến IFNAR1 ở người và mô tả một tình trạng suy giảm miễn dịch di gen đặc hiệu đối với IFN loại I và loại II."} +{"idx": 1930101, "en": "Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm's canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. Here, we describe roles for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By contrast, ANGPT2 was dispensable, although mice deficient in both Angpt1 and Angpt2 completely lacked SC, indicating that ANGPT2 compensates for the loss of ANGPT1. In addition, we identified 3 human subjects with rare ANGPT1 variants within an international cohort of 284 PCG patients. Loss of function in 2 of the 3 patient alleles was observed by functional analysis of ANGPT1 variants in a combined in silico, in vitro, and in vivo approach, supporting a causative role for ANGPT1 in disease. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.", "gemini": "Glôcôm bẩm sinh nguyên phát (PCG) là một nguyên nhân hàng đầu gây mù ở trẻ em trên toàn thế giới và là do các khuyết tật phát triển ở 2 cấu trúc dẫn lưu dịch nội nhãn, ống Schlemm (SC) và mạng lưới xốp. Trước đây, chúng tôi đã xác định các đột biến mất chức năng ở thụ thể angiopoietin (ANGPT) TEK trong các gia đình mắc PCG và chỉ ra rằng tín hiệu ANGPT/TEK rất cần thiết cho sự phát triển của SC. Ở đây, chúng tôi mô tả vai trò của các phối tử ANGPT chính trong sự phát triển của đường dẫn lưu dịch nội nhãn. Chúng tôi xác định rằng ANGPT1 rất cần thiết cho sự phát triển của SC và những con chuột bị loại bỏ Angpt1 tạo thành một ống cực kỳ kém phát triển với áp suất nội nhãn tăng cao. Ngược lại, ANGPT2 không cần thiết, mặc dù những con chuột thiếu cả Angpt1 và Angpt2 hoàn toàn không có SC, cho thấy ANGPT2 bù đắp cho sự mất ANGPT1. Ngoài ra, chúng tôi đã xác định được 3 đối tượng người có biến thể ANGPT1 hiếm trong số 284 bệnh nhân PCG trong một nhóm quốc tế. Sự mất chức năng ở 2 trong số 3 alen của bệnh nhân đã được quan sát thấy bằng phân tích chức năng các biến thể ANGPT1 theo cách tiếp cận kết hợp in silico, in vitro và in vivo, hỗ trợ vai trò gây bệnh của ANGPT1 trong bệnh. Bằng cách liên kết ANGPT1 với PCG, những kết quả này làm nổi bật tầm quan trọng của tín hiệu ANGPT/TEK trong bệnh sinh của bệnh tăng nhãn áp và xác định một mục tiêu ứng viên để phát triển liệu pháp điều trị."} +{"idx": 1930102, "en": "Molybdenum cofactor deficiency (MoCD) is an autosomal recessive inborn error of metabolism characterized by neurodegeneration and death in early childhood. The rapid and progressive neurodegeneration in MoCD presents a major clinical challenge and may relate to the poor understanding of the molecular mechanisms involved. Recently, we reported that treating patients with cyclic pyranopterin monophosphate (cPMP) is a successful therapy for a subset of infants with MoCD and prevents irreversible brain damage. Here, we studied S-sulfocysteine (SSC), a structural analog of glutamate that accumulates in the plasma and urine of patients with MoCD, and demonstrated that it acts as an N-methyl D-aspartate receptor (NMDA-R) agonist, leading to calcium influx and downstream cell signaling events and neurotoxicity. SSC treatment activated the protease calpain, and calpain-dependent degradation of the inhibitory synaptic protein gephyrin subsequently exacerbated SSC-mediated excitotoxicity and promoted loss of GABAergic synapses. Pharmacological blockade of NMDA-R, calcium influx, or calpain activity abolished SSC and glutamate neurotoxicity in primary murine neurons. Finally, the NMDA-R antagonist memantine was protective against the manifestation of symptoms in a tungstate-induced MoCD mouse model. These findings demonstrate that SSC drives excitotoxic neurodegeneration in MoCD and introduce NMDA-R antagonists as potential therapeutics for this fatal disease.", "gemini": "Thiếu hụt cofactor Molybdenum (MoCD) là một lỗi bẩm sinh lặn trên nhiễm sắc thể thường, đặc trưng bởi thoái hóa thần kinh và tử vong ở trẻ nhỏ. Thoái hóa thần kinh nhanh chóng và tiến triển ở MoCD là một thách thức lâm sàng lớn và có thể liên quan đến sự hiểu biết kém về các cơ chế phân tử liên quan. Gần đây, chúng tôi đã báo cáo rằng việc điều trị bệnh nhân bằng pyranopterin monophosphate chu kỳ (cPMP) là một liệu pháp thành công cho một nhóm trẻ sơ sinh mắc MoCD và ngăn ngừa tổn thương não không hồi phục. Ở đây, chúng tôi nghiên cứu S-sulfocysteine (SSC), một chất tương tự về cấu trúc của glutamate tích tụ trong huyết tương và nước tiểu của bệnh nhân MoCD, và chứng minh rằng nó hoạt động như một chất chủ vận thụ thể N-methyl D-aspartate (NMDA-R), dẫn đến dòng canxi và các sự kiện truyền tín hiệu tế bào hạ nguồn và độc tính thần kinh. Xử lý SSC kích hoạt protease calpain và sự thoái hóa phụ thuộc calpain của protein ức chế synap gephyrin sau đó làm trầm trọng thêm độc tính kích thích do SSC gây ra và thúc đẩy mất các khớp thần kinh GABAergic. Việc phong tỏa dược lý NMDA-R, dòng canxi hoặc hoạt động của calpain đã xóa bỏ độc tính thần kinh do SSC và glutamate ở các tế bào thần kinh chuột chính. Cuối cùng, chất đối kháng NMDA-R memantine có tác dụng bảo vệ chống lại biểu hiện các triệu chứng trong mô hình chuột MoCD do vonfram gây ra. Những phát hiện này chứng minh rằng SSC thúc đẩy thoái hóa thần kinh kích thích trong MoCD và đưa ra các chất đối kháng NMDA-R như là liệu pháp điều trị tiềm năng cho căn bệnh gây tử vong này."} +{"idx": 1930103, "en": "Nonalcoholic steatohepatitis (NASH) is characterized by progressive liver injury, inflammation, and fibrosis; however, the mechanisms that govern the transition from hepatic steatosis, which is relatively benign, to NASH remain poorly defined. Neuregulin 4 (Nrg4) is an adipose tissue-enriched endocrine factor that elicits beneficial metabolic effects in obesity. Here, we show that Nrg4 is a key component of an endocrine checkpoint that preserves hepatocyte health and counters diet-induced NASH in mice. Nrg4 deficiency accelerated liver injury, fibrosis, inflammation, and cell death in a mouse model of NASH. In contrast, transgenic expression of Nrg4 in adipose tissue alleviated diet-induced NASH. Nrg4 attenuated hepatocyte death in a cell-autonomous manner by blocking ubiquitination and proteasomal degradation of c-FLIPL, a negative regulator of cell death. Adeno-associated virus-mediated (AAV-mediated) rescue of hepatic c-FLIPL expression in Nrg4-deficent mice functionally restored the brake for steatosis to NASH transition. Thus, hepatic Nrg4 signaling serves as an endocrine checkpoint for steatosis-to-NASH progression by activating a cytoprotective pathway to counter stress-induced liver injury.", "gemini": "Viêm gan nhiễm mỡ không do rượu (NASH) được đặc trưng bởi tổn thương gan tiến triển, viêm và xơ hóa; tuy nhiên, các cơ chế điều chỉnh quá trình chuyển đổi từ gan nhiễm mỡ, tương đối lành tính, sang NASH vẫn chưa được xác định rõ. Neuregulin 4 (Nrg4) là một yếu tố nội tiết làm giàu mô mỡ có tác dụng có lợi cho quá trình trao đổi chất ở bệnh béo phì. Ở đây, chúng tôi chỉ ra rằng Nrg4 là một thành phần chính của một trạm kiểm soát nội tiết giúp bảo vệ sức khỏe tế bào gan và chống lại NASH do chế độ ăn uống ở chuột. Sự thiếu hụt Nrg4 làm tăng nhanh tổn thương gan, xơ hóa, viêm và chết tế bào trong mô hình NASH ở chuột. Ngược lại, việc biểu hiện gen Nrg4 ở mô mỡ làm giảm NASH do chế độ ăn uống. Nrg4 làm giảm chết tế bào gan theo cách tự chủ của tế bào bằng cách ngăn chặn ubiquitin hóa và thoái hóa proteasome của c-FLIPL, một chất điều hòa tiêu cực của quá trình chết tế bào. Việc giải cứu biểu hiện c-FLIPL ở gan do virus liên kết với adeno (AAV) ở những con chuột thiếu Nrg4 đã khôi phục chức năng phanh cho quá trình chuyển đổi từ gan nhiễm mỡ sang NASH. Do đó, tín hiệu Nrg4 ở gan đóng vai trò là trạm kiểm soát nội tiết cho quá trình tiến triển từ gan nhiễm mỡ sang NASH bằng cách kích hoạt một con đường bảo vệ tế bào để chống lại tổn thương gan do stress gây ra."} +{"idx": 1930104, "en": "Astrocytes perform critical non-cell autonomous roles following CNS injury that involve either neurotoxic or neuroprotective effects. Yet the nature of potential prosurvival cues has remained unclear. In the current study, we utilized the close interaction between astrocytes and retinal ganglion cells (RGCs) in the eye to characterize a secreted neuroprotective signal present in retinal astrocyte conditioned medium (ACM). Rather than a conventional peptide neurotrophic factor, we identified a prominent lipid component of the neuroprotective signal through metabolomics screening. The lipoxins LXA4 and LXB4 are small lipid mediators that act locally to dampen inflammation, but they have not been linked directly to neuronal actions. Here, we determined that LXA4 and LXB4 are synthesized in the inner retina, but their levels are reduced following injury. Injection of either lipoxin was sufficient for neuroprotection following acute injury, while inhibition of key lipoxin pathway components exacerbated injury-induced damage. Although LXA4 signaling has been extensively investigated, LXB4, the less studied lipoxin, emerged to be more potent in protection. Moreover, LXB4 neuroprotection was different from that of established LXA4 signaling, and therapeutic LXB4 treatment was efficacious in a chronic model of the common neurodegenerative disease glaucoma. Together, these results identify a potential paracrine mechanism that coordinates neuronal homeostasis and inflammation in the CNS.", "gemini": "Các tế bào hình sao thực hiện các vai trò không tự chủ của tế bào quan trọng sau chấn thương CNS liên quan đến tác dụng độc thần kinh hoặc bảo vệ thần kinh. Tuy nhiên, bản chất của các tín hiệu bảo vệ sự sống tiềm ẩn vẫn chưa rõ ràng. Trong nghiên cứu hiện tại, chúng tôi đã sử dụng tương tác chặt chẽ giữa các tế bào hình sao và các tế bào hạch võng mạc (RGC) trong mắt để mô tả một tín hiệu bảo vệ thần kinh tiết ra có trong môi trường nuôi cấy tế bào hình sao võng mạc (ACM). Thay vì một yếu tố dinh dưỡng thần kinh peptide thông thường, chúng tôi đã xác định được một thành phần lipid nổi bật của tín hiệu bảo vệ thần kinh thông qua sàng lọc chuyển hóa. Lipoxins LXA4 và LXB4 là các chất trung gian lipid nhỏ có tác dụng tại chỗ để làm giảm viêm, nhưng chúng chưa được liên kết trực tiếp với các hoạt động của tế bào thần kinh. Ở đây, chúng tôi xác định rằng LXA4 và LXB4 được tổng hợp ở võng mạc bên trong, nhưng mức độ của chúng giảm sau chấn thương. Việc tiêm bất kỳ loại lipoxin nào cũng đủ để bảo vệ thần kinh sau chấn thương cấp tính, trong khi việc ức chế các thành phần chính của con đường lipoxin làm trầm trọng thêm tổn thương do chấn thương gây ra. Mặc dù tín hiệu LXA4 đã được nghiên cứu rộng rãi, nhưng LXB4, loại lipoxin ít được nghiên cứu hơn, lại có khả năng bảo vệ mạnh hơn. Hơn nữa, bảo vệ thần kinh LXB4 khác với bảo vệ thần kinh LXA4 đã được thiết lập và điều trị LXB4 có hiệu quả trong mô hình mãn tính của bệnh thoái hóa thần kinh phổ biến là bệnh tăng nhãn áp. Cùng nhau, những kết quả này xác định một cơ chế cận tiết tiềm năng phối hợp cân bằng nội môi tế bào thần kinh và tình trạng viêm trong CNS."} +{"idx": 1930105, "en": "Ebolaviruses and marburgviruses belong to the family Filoviridae and cause high lethality in infected patients. There are currently no licensed filovirus vaccines or antiviral therapies. The development of broad-spectrum therapies against members of the Marburgvirus genus, including Marburg virus (MARV) and Ravn virus (RAVV), is difficult because of substantial sequence variability. RNAi therapeutics offer a potential solution, as identification of conserved target nucleotide sequences may confer activity across marburgvirus variants. Here, we assessed the therapeutic efficacy of lipid nanoparticle (LNP) delivery of a single nucleoprotein-targeting (NP-targeting) siRNA in nonhuman primates at advanced stages of MARV or RAVV disease to mimic cases in which patients begin treatment for fulminant disease. Sixteen rhesus monkeys were lethally infected with MARV or RAVV and treated with NP siRNA-LNP, with MARV-infected animals beginning treatment four or five days after infection and RAVV-infected animals starting treatment three or six days after infection. While all untreated animals succumbed to disease, NP siRNA-LNP treatment conferred 100% survival of RAVV-infected macaques, even when treatment began just 1 day prior to the death of the control animals. In MARV-infected animals, day-4 treatment initiation resulted in 100% survival, and day-5 treatment resulted in 50% survival. These results identify a single siRNA therapeutic that provides broad-spectrum protection against both MARV and RAVV.", "gemini": "Virus Ebola và virus Marburg thuộc họ Filoviridae và gây tử vong cao ở những bệnh nhân bị nhiễm. Hiện tại không có vắc-xin chống lại virus filo hoặc liệu pháp kháng vi-rút nào được cấp phép. Việc phát triển các liệu pháp phổ rộng chống lại các thành viên của chi Marburgvirus, bao gồm virus Marburg (MARV) và virus Ravn (RAVV), rất khó khăn vì trình tự biến đổi đáng kể. Liệu pháp RNAi cung cấp một giải pháp tiềm năng, vì việc xác định các trình tự nucleotide mục tiêu được bảo tồn có thể mang lại hoạt động trên các biến thể của virus Marburg. Tại đây, chúng tôi đã đánh giá hiệu quả điều trị của việc cung cấp hạt nano lipid (LNP) một siRNA nhắm mục tiêu nucleoprotein đơn (NP) ở các loài linh trưởng không phải người ở giai đoạn tiến triển của bệnh MARV hoặc RAVV để bắt chước các trường hợp bệnh nhân bắt đầu điều trị bệnh cấp tính. Mười sáu con khỉ rhesus bị nhiễm MARV hoặc RAVV gây tử vong và được điều trị bằng NP siRNA-LNP, với những con vật bị nhiễm MARV bắt đầu điều trị bốn hoặc năm ngày sau khi nhiễm và những con vật bị nhiễm RAVV bắt đầu điều trị ba hoặc sáu ngày sau khi nhiễm. Trong khi tất cả các động vật không được điều trị đều tử vong vì bệnh, thì điều trị bằng NP siRNA-LNP mang lại tỷ lệ sống sót 100% ở những con khỉ bị nhiễm RAVV, ngay cả khi điều trị bắt đầu chỉ 1 ngày trước khi những con vật đối chứng chết. Ở những động vật bị nhiễm MARV, việc bắt đầu điều trị vào ngày thứ 4 dẫn đến tỷ lệ sống sót 100% và điều trị vào ngày thứ 5 dẫn đến tỷ lệ sống sót 50%. Những kết quả này xác định một liệu pháp siRNA đơn cung cấp khả năng bảo vệ phổ rộng chống lại cả MARV và RAVV."} +{"idx": 1930106, "en": "Glucagon plays a major role in the regulation of glucose homeostasis during fed and fasting states. However, the mechanisms responsible for the regulation of pancreatic α cell mass and function are not completely understood. In the current study, we identified mTOR complex 1 (mTORC1) as a major regulator of α cell mass and glucagon secretion. Using mice with tissue-specific deletion of the mTORC1 regulator Raptor in α cells (αRaptorKO), we showed that mTORC1 signaling is dispensable for α cell development, but essential for α cell maturation during the transition from a milk-based diet to a chow-based diet after weaning. Moreover, inhibition of mTORC1 signaling in αRaptorKO mice and in WT animals exposed to chronic rapamycin administration decreased glucagon content and glucagon secretion. In αRaptorKO mice, impaired glucagon secretion occurred in response to different secretagogues and was mediated by alterations in KATP channel subunit expression and activity. Additionally, our data identify the mTORC1/FoxA2 axis as a link between mTORC1 and transcriptional regulation of key genes responsible for α cell function. Thus, our results reveal a potential function of mTORC1 in nutrient-dependent regulation of glucagon secretion and identify a role for mTORC1 in controlling α cell-mass maintenance.", "gemini": "Glucagon đóng vai trò chính trong quá trình điều hòa cân bằng glucose trong trạng thái ăn và nhịn ăn. Tuy nhiên, các cơ chế chịu trách nhiệm điều hòa khối lượng và chức năng tế bào α tuyến tụy vẫn chưa được hiểu đầy đủ. Trong nghiên cứu hiện tại, chúng tôi đã xác định phức hợp mTOR 1 (mTORC1) là một chất điều hòa chính của khối lượng tế bào α và tiết glucagon. Sử dụng chuột bị xóa gen điều hòa mTORC1 Raptor đặc hiệu mô ở tế bào α (αRaptorKO), chúng tôi đã chứng minh rằng tín hiệu mTORC1 không thể thiếu đối với sự phát triển của tế bào α, nhưng rất cần thiết cho sự trưởng thành của tế bào α trong quá trình chuyển đổi từ chế độ ăn dựa trên sữa sang chế độ ăn dựa trên thức ăn sau khi cai sữa. Hơn nữa, việc ức chế tín hiệu mTORC1 ở chuột αRaptorKO và ở động vật WT tiếp xúc với chế độ dùng rapamycin mạn tính làm giảm hàm lượng glucagon và tiết glucagon. Ở chuột αRaptorKO, tiết glucagon bị suy giảm xảy ra để đáp ứng với các chất tiết khác nhau và được trung gian bởi sự thay đổi trong biểu hiện và hoạt động của tiểu đơn vị kênh KATP. Ngoài ra, dữ liệu của chúng tôi xác định trục mTORC1/FoxA2 là một liên kết giữa mTORC1 và điều hòa phiên mã của các gen chính chịu trách nhiệm cho chức năng tế bào α. Do đó, kết quả của chúng tôi cho thấy chức năng tiềm năng của mTORC1 trong quá trình điều hòa tiết glucagon phụ thuộc vào chất dinh dưỡng và xác định vai trò của mTORC1 trong việc kiểm soát duy trì khối lượng tế bào α."} +{"idx": 1930107, "en": "NUAK1 is a member of the AMPK-related family of kinases. Recent evidence suggests that NUAK1 is an important regulator of cell adhesion and migration, cellular and organismal metabolism, and regulation of TAU stability. As such, NUAK1 may play key roles in multiple diseases ranging from neurodegeneration to diabetes and metastatic cancer. Previous work revealed a crucial role for NUAK1 in supporting viability of tumour cells specifically when MYC is overexpressed. This role is surprising, given that NUAK1 is activated by the tumour suppressor LKB1. Here we show that, in tumour cells lacking LKB1, NUAK1 activity is maintained by an alternative pathway involving calcium-dependent activation of PKCα. Calcium/PKCα-dependent activation of NUAK1 supports engagement of the AMPK-TORC1 metabolic checkpoint, thereby protecting tumour cells from MYC-driven cell death, and indeed, MYC selects for this pathway in part via transcriptional regulation of PKCα and ITPR. Our data point to a novel role for calcium in supporting tumour cell viability and clarify the synthetic lethal interaction between NUAK1 and MYC.", "gemini": "NUAK1 là một thành viên của họ kinase liên quan đến AMPK. Các bằng chứng gần đây cho thấy NUAK1 là một chất điều hòa quan trọng của quá trình kết dính và di chuyển tế bào, quá trình trao đổi chất của tế bào và cơ thể, và điều hòa sự ổn định của TAU. Như vậy, NUAK1 có thể đóng vai trò chính trong nhiều bệnh, từ thoái hóa thần kinh đến tiểu đường và ung thư di căn. Các nghiên cứu trước đây đã chỉ ra vai trò quan trọng của NUAK1 trong việc hỗ trợ khả năng sống của các tế bào khối u, đặc biệt là khi MYC được biểu hiện quá mức. Vai trò này thật đáng ngạc nhiên, vì NUAK1 được kích hoạt bởi chất ức chế khối u LKB1. Ở đây chúng tôi chỉ ra rằng, trong các tế bào khối u thiếu LKB1, hoạt động của NUAK1 được duy trì bởi một con đường thay thế liên quan đến hoạt hóa PKCα phụ thuộc canxi. Hoạt hóa NUAK1 phụ thuộc canxi/PKCα hỗ trợ sự tham gia của trạm kiểm soát chuyển hóa AMPK-TORC1, do đó bảo vệ các tế bào khối u khỏi quá trình chết tế bào do MYC thúc đẩy và thực sự, MYC chọn con đường này một phần thông qua điều hòa phiên mã của PKCα và ITPR. Dữ liệu của chúng tôi chỉ ra một vai trò mới của canxi trong việc hỗ trợ khả năng sống của tế bào khối u và làm rõ tương tác gây tử vong tổng hợp giữa NUAK1 và MYC."} +{"idx": 1930108, "en": "Cullin 4B (CUL4B) is a scaffold protein overexpressed in several solid malignancies. It is known to silence tumor suppressor through post-transcriptional manner. However, its clinical significance and underlying molecular mechanisms in gastric cancer (GC) remain largely unknown. In this study, we found that CUL4B was significantly overexpressed in GC tissues and its overexpression was correlated with lymph node metastasis and poor prognosis. Through gain- and loss-of-function experiments, we showed that CUL4B promotes GC cell invasion and epithelial-mesenchymal transition (EMT) in vitro, as well as tumor growth and metastasis in vivo. Mechanistically, we identified HER2 as a downstream target gene of CUL4B in GC. CUL4B unregulated HER2 expression via transcriptionally repressing miR-125a. Intriguingly, HER2 inhibitors significantly reversed CUL4B-induced EMT in vitro and partially blocked GC metastasis in tumor xenografts with CUL4B overexpression. Finally, we suggested the involvement of the PI3K/AKT pathway in CUL4B-induced HER2 upregulation in GC. In all, we proposed a model for a CUL4B-miR-125a-HER2 oncoprotein axis, which provided novel insight into how HER2 was activated and contributed to GC progression and metastasis.", "gemini": "Cullin 4B (CUL4B) là một protein khung được biểu hiện quá mức trong một số khối u ác tính rắn. Nó được biết là làm im lặng các gen ức chế khối u thông qua phương thức hậu phiên mã. Tuy nhiên, ý nghĩa lâm sàng và cơ chế phân tử cơ bản của nó trong ung thư dạ dày (GC) vẫn chưa được biết rõ. Trong nghiên cứu này, chúng tôi thấy rằng CUL4B được biểu hiện quá mức đáng kể trong các mô GC và sự biểu hiện quá mức của nó có liên quan đến di căn hạch bạch huyết và tiên lượng kém. Thông qua các thí nghiệm tăng và mất chức năng, chúng tôi đã chứng minh rằng CUL4B thúc đẩy sự xâm lấn của tế bào GC và quá trình chuyển đổi biểu mô-trung mô (EMT) trong ống nghiệm, cũng như sự phát triển và di căn của khối u trong cơ thể sống. Về mặt cơ chế, chúng tôi xác định HER2 là gen đích hạ nguồn của CUL4B trong GC. CUL4B điều hòa biểu hiện HER2 thông qua việc ức chế phiên mã miR-125a. Thật thú vị, các chất ức chế HER2 đảo ngược đáng kể EMT do CUL4B gây ra trong ống nghiệm và chặn một phần di căn GC trong các khối u ghép dị chủng với sự biểu hiện quá mức CUL4B. Cuối cùng, chúng tôi đề xuất sự tham gia của con đường PI3K/AKT trong việc điều hòa tăng HER2 do CUL4B gây ra trong GC. Nhìn chung, chúng tôi đề xuất một mô hình cho trục oncoprotein CUL4B-miR-125a-HER2, cung cấp cái nhìn sâu sắc mới về cách HER2 được kích hoạt và góp phần vào sự tiến triển và di căn của GC."} +{"idx": 1930109, "en": "Glycolysis is critical for cancer stem cell reprogramming; however, the underlying regulatory mechanisms remain elusive. Here, we show that pyruvate dehydrogenase kinase 1 (PDK1) is enriched in breast cancer stem cells (BCSCs), whereas depletion of PDK1 remarkably diminishes ALDH+ subpopulations, decreases stemness-related transcriptional factor expression, and inhibits sphere-formation ability and tumor growth. Conversely, high levels of PDK1 enhance BCSC properties and are correlated with poor overall survival. In mouse xenograft tumor, PDK1 is accumulated in hypoxic regions and activates glycolysis to promote stem-like traits. Moreover, through screening hypoxia-related long non-coding RNAs (lncRNAs) in PDK1-positive tissue, we find that lncRNA H19 is responsible for glycolysis and BCSC maintenance. Furthermore, H19 knockdown decreases PDK1 expression in hypoxia, and ablation of PDK1 counteracts H19-mediated glycolysis and self-renewal ability in vitro and in vivo. Accordingly, H19 and PDK1 expression exhibits strong correlations in primary breast carcinomas. H19 acting as a competitive endogenous RNA sequesters miRNA let-7 to release Hypoxia-inducible factor 1α, leading to an increase in PDK1 expression. Lastly, aspirin markedly attenuates glycolysis and cancer stem-like characteristics by suppressing both H19 and PDK1. Thus, these novel findings demonstrate that the glycolysis gatekeeper PDK1 has a critical role in BCSC reprogramming and provides a potential therapeutic strategy for breast malignancy.", "gemini": "Quá trình đường phân rất quan trọng đối với quá trình tái lập trình tế bào gốc ung thư; tuy nhiên, các cơ chế điều hòa cơ bản vẫn còn khó nắm bắt. Ở đây, chúng tôi chỉ ra rằng pyruvate dehydrogenase kinase 1 (PDK1) được làm giàu trong các tế bào gốc ung thư vú (BCSC), trong khi sự suy giảm PDK1 làm giảm đáng kể các quần thể phụ ALDH+, làm giảm biểu hiện yếu tố phiên mã liên quan đến tính gốc và ức chế khả năng hình thành khối cầu và sự phát triển của khối u. Ngược lại, nồng độ PDK1 cao làm tăng các đặc tính BCSC và có liên quan đến tỷ lệ sống sót chung kém. Trong khối u ghép dị chủng ở chuột, PDK1 tích tụ ở các vùng thiếu oxy và kích hoạt quá trình đường phân để thúc đẩy các đặc điểm giống tế bào gốc. Hơn nữa, thông qua việc sàng lọc các RNA không mã hóa dài (lncRNA) liên quan đến tình trạng thiếu oxy trong mô dương tính với PDK1, chúng tôi thấy rằng lncRNA H19 chịu trách nhiệm cho quá trình đường phân và duy trì BCSC. Hơn nữa, việc hạ gục H19 làm giảm biểu hiện PDK1 trong tình trạng thiếu oxy và việc loại bỏ PDK1 chống lại quá trình đường phân do H19 trung gian và khả năng tự tái tạo trong ống nghiệm và trong cơ thể sống. Theo đó, biểu hiện của H19 và PDK1 có mối tương quan mạnh trong các ung thư biểu mô vú nguyên phát. H19 hoạt động như một RNA nội sinh cạnh tranh cô lập miRNA let-7 để giải phóng yếu tố 1α có thể gây thiếu oxy, dẫn đến tăng biểu hiện PDK1. Cuối cùng, aspirin làm giảm đáng kể quá trình đường phân và các đặc điểm giống tế bào gốc ung thư bằng cách ức chế cả H19 và PDK1. Do đó, những phát hiện mới này chứng minh rằng PDK1 bảo vệ đường phân có vai trò quan trọng trong quá trình tái lập trình BCSC và cung cấp một chiến lược điều trị tiềm năng cho bệnh ác tính vú."} +{"idx": 1930110, "en": "Six GATA transcription factors play important roles in eukaryotic development. Among these, GATA2, an essential factor for the hematopoietic cell lineage, exhibits low expression in human gastric tissues, whereas GATA6, which is crucial for gastrointestinal development and differentiation, is frequently amplified and/or overexpressed in human gastric cancer. Interestingly, we found that GATA6 was overexpressed in human gastric cancer cells only when GATA2 expression was completely absent, thereby showing an inverse correlation between GATA2 and GATA6. In gastric cancer cells that express high GATA6 levels, a GATA2 CpG island is hypermethylated, repressing expression in these cells. In contrast, GATA6 expression is undetectable in GATA2-overexpressing gastric cancer cells, which lack GATA2 DNA methylation. Furthermore, PRC2 complex-mediated transcriptional silencing of GATA6 was observed in the GATA2-overexpressing cells. We also show that the GATA2 and PRC2 complexes are enriched within the GATA6 locus, and that the recruitment of the PRC2 complex is impaired by disrupting GATA2 expression, resulting in GATA6 upregulation. In addition, ectopic GATA2 expression significantly downregulates GATA6 expression, suggesting GATA2 directly represses GATA6. Furthermore, GATA6 downregulation showed antitumor activity by inducing growth arrest. Finally, we show that aberrant GATA2 methylation occurs early during the multistep process of gastric carcinogenesis regardless of Helicobacter pylori infection. Taken together, GATA2 dysregulation by epigenetic modification is associated with unfavorable phenotypes in human gastric cancer cells by allowing GATA6 expression.", "gemini": "Sáu yếu tố phiên mã GATA đóng vai trò quan trọng trong quá trình phát triển của eukaryote. Trong số này, GATA2, một yếu tố thiết yếu cho dòng tế bào tạo máu, biểu hiện thấp trong các mô dạ dày của người, trong khi GATA6, rất quan trọng đối với sự phát triển và biệt hóa của đường tiêu hóa, thường được khuếch đại và/hoặc biểu hiện quá mức trong ung thư dạ dày của người. Thật thú vị, chúng tôi thấy rằng GATA6 được biểu hiện quá mức trong các tế bào ung thư dạ dày của người chỉ khi biểu hiện GATA2 hoàn toàn không có, do đó cho thấy mối tương quan nghịch giữa GATA2 và GATA6. Trong các tế bào ung thư dạ dày có mức GATA6 cao, đảo CpG GATA2 bị tăng methyl hóa, ức chế biểu hiện trong các tế bào này. Ngược lại, biểu hiện GATA6 không thể phát hiện thấy trong các tế bào ung thư dạ dày biểu hiện quá mức GATA2, những tế bào này thiếu methyl hóa DNA GATA2. Hơn nữa, sự im lặng phiên mã của GATA6 do phức hợp PRC2 trung gian đã được quan sát thấy trong các tế bào biểu hiện quá mức GATA2. Chúng tôi cũng chỉ ra rằng các phức hợp GATA2 và PRC2 được làm giàu trong locus GATA6 và việc tuyển dụng phức hợp PRC2 bị suy yếu do phá vỡ biểu hiện GATA2, dẫn đến sự điều hòa tăng GATA6. Ngoài ra, biểu hiện GATA2 ngoại sinh làm giảm đáng kể biểu hiện GATA6, cho thấy GATA2 trực tiếp ức chế GATA6. Hơn nữa, việc hạ điều hòa GATA6 cho thấy hoạt tính chống khối u bằng cách gây ra sự ngừng phát triển. Cuối cùng, chúng tôi chỉ ra rằng sự methyl hóa GATA2 bất thường xảy ra sớm trong quá trình nhiều bước gây ung thư dạ dày bất kể tình trạng nhiễm Helicobacter pylori. Tóm lại, sự rối loạn điều hòa GATA2 do sửa đổi biểu sinh có liên quan đến các kiểu hình không thuận lợi trong các tế bào ung thư dạ dày của người bằng cách cho phép biểu hiện GATA6."} +{"idx": 1930111, "en": "IL-37, a newly found anti-inflammatory cytokine of the IL-1 family, has both extracellular and intracellular functions. Accumulating evidences indicate that it is also involved in tumor progression. However, the mechanism and its intracellular target are unclear. In this study, clinical data from 84 patients showed that loss or reduced expression of IL-37 in lung adenocarcinoma tissues was significantly associated with tumor metastasis. We further provided evidence that IL-37 inhibited effectively tumor metastasis in vitro and in vivo. Moreover, we uncovered a novel mechanism by which IL-37 suppressed tumor cell migration via its intracellular mature form (amino acids 46-218). Intracellular mature form of IL-37, but not its extracellular form, markedly inhibited migration of multiple kinds of tumor cells through inhibiting Rac1 activation. Mechanistically, intracellular mature IL-37 directly bound to the CAAX motif in the C-terminal hypervariable region of Rac1, and then inhibited Rac1 membrane translocation and subsequent downstream signaling. Our research identifies intracellular mature IL-37 as a novel endogenous inhibitor of Rac1. Given the crucial roles of Rac1 in tumor angiogenesis and metastasis, intracellular mature IL-37 might serve as a potential strategy for the control of Rac1 activity and tumor progression.", "gemini": "IL-37, một cytokine chống viêm mới được tìm thấy của họ IL-1, có chức năng cả ngoại bào và nội bào. Các bằng chứng tích lũy chỉ ra rằng nó cũng liên quan đến sự tiến triển của khối u. Tuy nhiên, cơ chế và mục tiêu nội bào của nó vẫn chưa rõ ràng. Trong nghiên cứu này, dữ liệu lâm sàng từ 84 bệnh nhân cho thấy sự mất hoặc giảm biểu hiện của IL-37 trong các mô ung thư biểu mô tuyến phổi có liên quan đáng kể đến di căn của khối u. Chúng tôi tiếp tục cung cấp bằng chứng rằng IL-37 ức chế hiệu quả di căn của khối u trong ống nghiệm và trong cơ thể sống. Hơn nữa, chúng tôi đã phát hiện ra một cơ chế mới mà theo đó IL-37 ức chế sự di chuyển của tế bào khối u thông qua dạng trưởng thành nội bào của nó (axit amin 46-218). Dạng trưởng thành nội bào của IL-37, nhưng không phải dạng ngoại bào của nó, ức chế rõ rệt sự di chuyển của nhiều loại tế bào khối u thông qua việc ức chế hoạt hóa Rac1. Về mặt cơ chế, IL-37 trưởng thành nội bào liên kết trực tiếp với mô típ CAAX trong vùng siêu biến đổi đầu C của Rac1, sau đó ức chế sự chuyển vị màng Rac1 và tín hiệu hạ nguồn tiếp theo. Nghiên cứu của chúng tôi xác định IL-37 trưởng thành nội bào là một chất ức chế nội sinh mới của Rac1. Với vai trò quan trọng của Rac1 trong quá trình hình thành mạch máu khối u và di căn, IL-37 trưởng thành nội bào có thể đóng vai trò là một chiến lược tiềm năng để kiểm soát hoạt động của Rac1 và sự tiến triển của khối u."} +{"idx": 1930112, "en": "Esophageal squamous cell carcinoma (ESCC) is highly prevailing in Asia and it is ranked in the most aggressive squamous cell carcinomas. High-frequency loss of heterozygosity occurred in chromosome 14q11.2 in many tumors including ESCC, suggesting that one or more tumor-suppressor genes might exist within this region. In this study, we identified the tumor-suppressing role of DHRS2 (short-chain dehydrogenase/reductase family, member 2) at 14q11.2 in ESCCs. Downregulation of DHRS2 occurred in 30.8% of primary ESCC tumor tissues vs paired non-tumorous tissues. DHRS2 downregulation was associated significantly with ESCC invasion, lymph nodes metastasis and clinical staging (P<0.001). Survival analysis revealed that DHRS2 downregulation was significantly associated with worse outcome of patients with ESCC. In vitro and in vivo studies indicated that both DHRS2 variants could suppress cell proliferation and cell motility. Moreover, we demonstrated that DHRS2 could reduce reactive oxygen species and decrease nicotinamide adenine dinucleotide phosphate (oxidized/reduced), increase p53 stability and decrease Rb phosphorylation; it also decreased p38 mitogen-activated protein kinase phosphorylation and matrix metalloproteinase 2. In summary, these findings demonstrated that DHRS2 had an important part in ESCC development and progression.", "gemini": "Ung thư biểu mô tế bào vảy thực quản (ESCC) rất phổ biến ở Châu Á và được xếp vào loại ung thư biểu mô tế bào vảy hung dữ nhất. Mất dị hợp tử tần suất cao xảy ra ở nhiễm sắc thể 14q11.2 trong nhiều khối u bao gồm ESCC, cho thấy một hoặc nhiều gen ức chế khối u có thể tồn tại trong vùng này. Trong nghiên cứu này, chúng tôi đã xác định vai trò ức chế khối u của DHRS2 (họ dehydrogenase/reductase chuỗi ngắn, thành viên 2) tại 14q11.2 trong ESCC. Sự điều hòa giảm DHRS2 xảy ra ở 30,8% các mô khối u ESCC nguyên phát so với các mô không phải khối u ghép đôi. Sự điều hòa giảm DHRS2 có liên quan đáng kể đến sự xâm lấn ESCC, di căn hạch bạch huyết và giai đoạn lâm sàng (P < 0,001). Phân tích sự sống còn cho thấy sự điều hòa giảm DHRS2 có liên quan đáng kể đến kết cục xấu hơn của bệnh nhân ESCC. Các nghiên cứu trong ống nghiệm và trong cơ thể sống chỉ ra rằng cả hai biến thể DHRS2 đều có thể ức chế sự tăng sinh tế bào và khả năng vận động của tế bào. Hơn nữa, chúng tôi đã chứng minh rằng DHRS2 có thể làm giảm các loài oxy phản ứng và làm giảm dinucleotide adenin nicotinamide phosphat (oxy hóa/khử), tăng độ ổn định của p53 và giảm phosphoryl hóa Rb; nó cũng làm giảm phosphoryl hóa protein kinase hoạt hóa mitogen p38 và metaloproteinase ma trận 2. Tóm lại, những phát hiện này chứng minh rằng DHRS2 có một phần quan trọng trong sự phát triển và tiến triển của ESCC."} +{"idx": 1930113, "en": "The goal of this study was to elucidate the functional role of Nox4 during acute kidney injury (AKI). NADPH oxidases are a major source of reactive oxygen species (ROS) in the kidney in normal and pathological conditions. Among NADPH oxidase isoforms, NADPH oxidase4 (Nox4) is highly expressed in the kidney and has an important role in kidney diseases, such as diabetic nephropathy and renal carcinoma. We previously found that Nox4 expression significantly increased in the toxic AKI model. However, its functional role and mechanism of action in AKI are still unknown. We scavenged ROS with apocynin in vitro and in vivo and found it attenuated cisplatin-triggered renal function decline. It also alleviated programmed cell death and renal inflammation, indicating a critical role for ROS in mediating AKI. Nox4 protein and mRNA levels were substantially upregulated by cisplatin in vivo and in vitro. Nox4 knockdown alleviated cisplatin-induced cell death and inflammatory response, while Nox4 overexpression aggravated them. Moreover, N-acetyl-L-cysteine (NAC)-mediated inhibition of ROS suppressed cell injury led by Nox4 overexpression, indicating Nox4-mediated ROS generation may be the key mediator in cisplatin-induced nephrotoxicity. Mechanistically, excessive expression of Nox4 induced programmed cell death, especially RIP-mediated necroptosis. Finally, we tested whether Nox4 is a potential therapeutic target using an AKI mouse model by injecting a lentivirus-packaged Nox4 shRNA plasmid through tail vein. Disruption of Nox4 led to renal function recovery, kidney damage relief and reduced inflammation. We conclude that Nox4 aggravates cisplatin-induced nephrotoxicity by promoting ROS-mediated programmed cell death and inflammation. Thus Nox4 may serve as a potential therapeutic target in the treatment of AKI.", "gemini": "Mục tiêu của nghiên cứu này là làm sáng tỏ vai trò chức năng của Nox4 trong tổn thương thận cấp (AKI). Oxidase NADPH là nguồn chính của các loài oxy phản ứng (ROS) trong thận trong điều kiện bình thường và bệnh lý. Trong số các đồng dạng oxidase NADPH, oxidase NADPH4 (Nox4) được biểu hiện nhiều trong thận và có vai trò quan trọng trong các bệnh về thận, chẳng hạn như bệnh thận đái tháo đường và ung thư biểu mô thận. Trước đây, chúng tôi đã phát hiện ra rằng biểu hiện Nox4 tăng đáng kể trong mô hình AKI độc hại. Tuy nhiên, vai trò chức năng và cơ chế hoạt động của nó trong AKI vẫn chưa được biết rõ. Chúng tôi đã thu gom ROS bằng apocynin trong ống nghiệm và trong cơ thể sống và thấy rằng nó làm giảm sự suy giảm chức năng thận do cisplatin gây ra. Nó cũng làm giảm chết tế bào theo chương trình và viêm thận, chỉ ra vai trò quan trọng của ROS trong việc trung gian AKI. Nồng độ protein và mRNA của Nox4 tăng đáng kể do cisplatin trong ống nghiệm và trong cơ thể sống. Nox4 knockdown làm giảm chết tế bào và đáp ứng viêm do cisplatin gây ra, trong khi Nox4 quá mức làm trầm trọng thêm chúng. Hơn nữa, ức chế ROS do N-acetyl-L-cysteine (NAC) trung gian ức chế tổn thương tế bào do Nox4 quá mức gây ra, chỉ ra rằng sự tạo ra ROS do Nox4 trung gian có thể là chất trung gian chính trong độc tính thận do cisplatin gây ra. Về mặt cơ chế, biểu hiện quá mức của Nox4 gây ra chết tế bào theo chương trình, đặc biệt là hoại tử tế bào do RIP trung gian. Cuối cùng, chúng tôi đã kiểm tra xem Nox4 có phải là mục tiêu điều trị tiềm năng hay không bằng cách sử dụng mô hình chuột AKI bằng cách tiêm plasmid shRNA Nox4 đóng gói lentivirus qua tĩnh mạch đuôi. Sự phá vỡ Nox4 dẫn đến phục hồi chức năng thận, giảm tổn thương thận và giảm viêm. Chúng tôi kết luận rằng Nox4 làm trầm trọng thêm độc tính thận do cisplatin gây ra bằng cách thúc đẩy chết tế bào theo chương trình do ROS trung gian và viêm. Do đó, Nox4 có thể đóng vai trò là mục tiêu điều trị tiềm năng trong điều trị AKI."} +{"idx": 1930114, "en": "Transcription is an essential process in all living cells. However, transcription also sensitises genomic DNA to damage from a number of endogenous sources. Although various mechanisms protect the integrity of DNA during transcription, transcription-associated genomic instability occurs in normal and malignant cells and, if unrepaired, can result in genomic alterations. Numerous studies have implicated genomic alterations found in cancer genomes to transcription. Hence, transcription-associated genomic instability can be considered as a major driver of cancer development. In this review, we summarise the body of knowledge on transcription-associated genomic instability and highlight recent discoveries in the field on both healthy and malignant cells. We also discuss how transcription-associated DNA damage might promote transforming lesions at cell type- and lineage-specific genes.", "gemini": "Phiên mã là một quá trình thiết yếu trong tất cả các tế bào sống. Tuy nhiên, phiên mã cũng làm cho DNA bộ gen trở nên nhạy cảm với tổn thương từ một số nguồn nội sinh. Mặc dù có nhiều cơ chế bảo vệ tính toàn vẹn của DNA trong quá trình phiên mã, nhưng sự bất ổn của bộ gen liên quan đến phiên mã xảy ra ở các tế bào bình thường và ác tính và nếu không được sửa chữa, có thể dẫn đến các biến đổi của bộ gen. Nhiều nghiên cứu đã liên quan đến các biến đổi của bộ gen tìm thấy trong bộ gen ung thư với phiên mã. Do đó, sự bất ổn của bộ gen liên quan đến phiên mã có thể được coi là một động lực chính của sự phát triển ung thư. Trong bài đánh giá này, chúng tôi tóm tắt kiến thức về sự bất ổn của bộ gen liên quan đến phiên mã và nêu bật những khám phá gần đây trong lĩnh vực này trên cả tế bào khỏe mạnh và tế bào ác tính. Chúng tôi cũng thảo luận về cách tổn thương DNA liên quan đến phiên mã có thể thúc đẩy các tổn thương biến đổi ở các gen đặc hiệu theo loại tế bào và dòng tế bào."} +{"idx": 1930115, "en": "Cobamides such as vitamin B12 are structurally conserved, cobalt-containing tetrapyrrole biomolecules that have essential biochemical functions in all domains of life. In organohalide respiration, a vital biological process for the global cycling of natural and anthropogenic organohalogens, cobamides are the requisite prosthetic groups for carbon-halogen bond-cleaving reductive dehalogenases. This study reports the biosynthesis of a new cobamide with unsubstituted purine as the lower base and assigns unsubstituted purine a biological function by demonstrating that Coα-purinyl-cobamide (purinyl-Cba) is the native prosthetic group in catalytically active tetrachloroethene reductive dehalogenases of Desulfitobacterium hafniense. Cobamides featuring different lower bases are not functionally equivalent, and purinyl-Cba elicits different physiological responses in corrinoid-auxotrophic, organohalide-respiring bacteria. Given that cobamide-dependent enzymes catalyze key steps in essential metabolic pathways, the discovery of a novel cobamide structure and the realization that lower bases can effectively modulate enzyme activities generate opportunities to manipulate functionalities of microbiomes.", "gemini": "Cobamide như vitamin B12 có cấu trúc bảo tồn, các biomolecule tetrapyrrole chứa coban có chức năng sinh hóa thiết yếu trong mọi lĩnh vực của sự sống. Trong quá trình hô hấp organohalide, một quá trình sinh học quan trọng đối với chu trình toàn cầu của các organohalogen tự nhiên và nhân tạo, cobamides là các nhóm giả bắt buộc đối với các dehalogenase khử liên kết carbon-halogen. Nghiên cứu này báo cáo quá trình sinh tổng hợp một loại cobamid mới với purin chưa thay thế làm bazơ thấp hơn và chỉ định purin chưa thay thế là chức năng sinh học bằng cách chứng minh rằng Coα-purinyl-cobamid (purinyl-Cba) là nhóm giả gốc trong các dehalogenase khử tetrachloroethylene có hoạt tính xúc tác của Desulfitobacterium hafniense. Các cobamid có các bazơ thấp hơn khác nhau không tương đương về mặt chức năng và purinyl-Cba gây ra các phản ứng sinh lý khác nhau ở vi khuẩn hô hấp organohalide, phụ thuộc vào corrinoid. Do các enzym phụ thuộc cobamid xúc tác các bước chính trong các con đường trao đổi chất thiết yếu, nên việc phát hiện ra cấu trúc cobamid mới và nhận ra rằng các bazơ thấp hơn có thể điều chỉnh hiệu quả hoạt động của enzym tạo ra cơ hội để thao tác các chức năng của hệ vi sinh vật."} +{"idx": 1930116, "en": "In principle, the millisecond emission lifetimes of lanthanide chelates should enable their ultrasensitive detection in biological systems by time-resolved optical microscopy. In practice, however, lanthanide imaging techniques have provided no better sensitivity than conventional fluorescence microscopy. Here, we identified three fundamental problems that have impeded lanthanide microscopy: low photon flux, inefficient excitation, and optics-derived background luminescence. We overcame these limitations with a new lanthanide imaging modality, transreflected illumination with luminescence resonance energy transfer (trLRET), which increases the time-integrated signal intensities of lanthanide lumiphores by 170-fold and the signal-to-background ratios by 75-fold. We demonstrate that trLRET provides at least an order-of-magnitude increase in detection sensitivity over that of conventional epifluorescence microscopy when used to visualize endogenous protein expression in zebrafish embryos. We also show that trLRET can be used to optically detect molecular interactions in vivo. trLRET promises to unlock the full potential of lanthanide lumiphores for ultrasensitive, autofluorescence-free biological imaging.", "gemini": "Về nguyên tắc, thời gian phát xạ mili giây của các chelat lanthanide sẽ cho phép phát hiện siêu nhạy của chúng trong các hệ thống sinh học bằng kính hiển vi quang học có độ phân giải thời gian. Tuy nhiên, trong thực tế, các kỹ thuật chụp ảnh lanthanide không cung cấp độ nhạy tốt hơn kính hiển vi huỳnh quang thông thường. Ở đây, chúng tôi xác định được ba vấn đề cơ bản đã cản trở kính hiển vi lanthanide: thông lượng photon thấp, kích thích không hiệu quả và phát quang nền có nguồn gốc từ quang học. Chúng tôi đã khắc phục những hạn chế này bằng một phương thức hình ảnh lanthanide mới, chiếu sáng phản xạ qua với sự truyền năng lượng cộng hưởng phát quang (trLRET), giúp tăng cường cường độ tín hiệu tích hợp thời gian của các lumiphore lanthanide lên 170 lần và tỷ lệ tín hiệu trên nhiễu nền lên 75 lần. Chúng tôi chứng minh rằng trLRET cung cấp ít nhất một độ lớn tăng độ nhạy phát hiện so với kính hiển vi huỳnh quang biểu bì thông thường khi được sử dụng để hình dung biểu hiện protein nội sinh trong phôi cá ngựa vằn. Chúng tôi cũng chỉ ra rằng trLRET có thể được sử dụng để phát hiện quang học các tương tác phân tử trong cơ thể sống. trLRET hứa hẹn sẽ mở khóa toàn bộ tiềm năng của các lumiphore lanthanide để chụp ảnh sinh học siêu nhạy, không tự phát quang."} +{"idx": 1930117, "en": "Asprosin is a recently discovered fasting-induced hormone that promotes hepatic glucose production. Here we demonstrate that asprosin in the circulation crosses the blood-brain barrier and directly activates orexigenic AgRP+ neurons via a cAMP-dependent pathway. This signaling results in inhibition of downstream anorexigenic proopiomelanocortin (POMC)-positive neurons in a GABA-dependent manner, which then leads to appetite stimulation and a drive to accumulate adiposity and body weight. In humans, a genetic deficiency in asprosin causes a syndrome characterized by low appetite and extreme leanness; this is phenocopied by mice carrying similar mutations and can be fully rescued by asprosin. Furthermore, we found that obese humans and mice had pathologically elevated concentrations of circulating asprosin, and neutralization of asprosin in the blood with a monoclonal antibody reduced appetite and body weight in obese mice, in addition to improving their glycemic profile. Thus, in addition to performing a glucogenic function, asprosin is a centrally acting orexigenic hormone that is a potential therapeutic target in the treatment of both obesity and diabetes.", "gemini": "Asprosin là một loại hormone do nhịn ăn gây ra mới được phát hiện gần đây có tác dụng thúc đẩy gan sản xuất glucose. Tại đây chúng tôi chứng minh rằng asprosin trong hệ tuần hoàn đi qua hàng rào máu não và kích hoạt trực tiếp các tế bào thần kinh AgRP+ gây thèm ăn thông qua một con đường phụ thuộc cAMP. Tín hiệu này dẫn đến sự ức chế các tế bào thần kinh proopiomelanocortin (POMC) dương tính gây chán ăn ở hạ nguồn theo cách phụ thuộc GABA, sau đó dẫn đến kích thích sự thèm ăn và thúc đẩy tích tụ mỡ và trọng lượng cơ thể. Ở người, tình trạng thiếu hụt asprosin do di truyền gây ra hội chứng đặc trưng bởi chán ăn và gầy cực độ; điều này được mô phỏng ở những con chuột mang đột biến tương tự và có thể được cứu chữa hoàn toàn bằng asprosin. Hơn nữa, chúng tôi phát hiện ra rằng những người béo phì và chuột béo phì có nồng độ asprosin lưu hành cao bất thường về mặt bệnh lý và việc trung hòa asprosin trong máu bằng kháng thể đơn dòng làm giảm sự thèm ăn và trọng lượng cơ thể ở chuột béo phì, ngoài ra còn cải thiện đường huyết của chúng. Do đó, ngoài chức năng sinh đường, asprosin là một loại hormone gây thèm ăn tác động lên trung tâm là mục tiêu điều trị tiềm năng trong điều trị cả bệnh béo phì và tiểu đường."} +{"idx": 1930118, "en": "Over 40% of microRNAs (miRNAs) are located in introns of protein-coding genes, and many of these intronic miRNAs are co-regulated with their host genes. In such cases of co-regulation, the products of host genes and their intronic miRNAs can cooperate to coordinately regulate biologically important pathways. Therefore, we screened intronic miRNAs dysregulated in the livers of mouse models of obesity to identify previously uncharacterized protein-coding host genes that may contribute to the pathogenesis of obesity-associated insulin resistance and type 2 diabetes mellitus. Our approach revealed that expression of both the gene encoding ectodysplasin A (Eda), the causal gene in X-linked hypohidrotic ectodermal dysplasia (XLHED), and its intronic miRNA, miR-676, was increased in the livers of obese mice. Moreover, hepatic EDA expression is increased in obese human subjects and reduced upon weight loss, and its hepatic expression correlates with systemic insulin resistance. We also found that reducing miR-676 expression in db/db mice increases the expression of proteins involved in fatty acid oxidation and reduces the expression of inflammatory signaling components in the liver. Further, we found that Eda expression in mouse liver is controlled via PPARγ and RXR-α, increases in circulation under conditions of obesity, and promotes JNK activation and inhibitory serine phosphorylation of IRS1 in skeletal muscle. In accordance with these findings, gain- and loss-of-function approaches reveal that liver-derived EDA regulates systemic glucose metabolism, suggesting that EDA is a hepatokine that can contribute to impaired skeletal muscle insulin sensitivity in obesity.", "gemini": "Hơn 40% microRNA (miRNA) nằm trong các intron của gen mã hóa protein và nhiều miRNA trong intron này được điều hòa cùng với gen chủ của chúng. Trong những trường hợp điều hòa cùng như vậy, các sản phẩm của gen chủ và miRNA trong intron của chúng có thể hợp tác để cùng nhau điều hòa các con đường quan trọng về mặt sinh học. Do đó, chúng tôi đã sàng lọc các miRNA trong intron bị rối loạn chức năng ở gan của các mô hình chuột mắc bệnh béo phì để xác định các gen chủ mã hóa protein chưa được mô tả trước đó có thể góp phần vào quá trình sinh bệnh của tình trạng kháng insulin liên quan đến béo phì và bệnh tiểu đường loại 2. Phương pháp tiếp cận của chúng tôi cho thấy rằng biểu hiện của cả gen mã hóa ectodysplasin A (Eda), gen gây bệnh trong chứng loạn sản ngoại bì do giảm tiết mồ hôi liên kết với X (XLHED) và miRNA trong intron của nó, miR-676, đều tăng ở gan của những con chuột béo phì. Hơn nữa, biểu hiện EDA ở gan tăng ở những người béo phì và giảm khi giảm cân, và biểu hiện ở gan của nó tương quan với tình trạng kháng insulin toàn thân. Chúng tôi cũng phát hiện ra rằng việc giảm biểu hiện miR-676 ở chuột db/db làm tăng biểu hiện của các protein liên quan đến quá trình oxy hóa axit béo và làm giảm biểu hiện của các thành phần tín hiệu viêm trong gan. Hơn nữa, chúng tôi phát hiện ra rằng biểu hiện Eda ở gan chuột được kiểm soát thông qua PPARγ và RXR-α, tăng trong hệ tuần hoàn trong tình trạng béo phì và thúc đẩy hoạt hóa JNK và phosphoryl hóa serine ức chế IRS1 ở cơ xương. Phù hợp với những phát hiện này, các phương pháp tiếp cận tăng và mất chức năng cho thấy rằng EDA có nguồn gốc từ gan điều hòa quá trình chuyển hóa glucose toàn thân, cho thấy rằng EDA là một loại hepatokine có thể góp phần làm suy giảm độ nhạy insulin của cơ xương trong tình trạng béo phì."} +{"idx": 1930119, "en": "Cancer-specific cell-surface antigens are ideal targets for monoclonal antibody (mAb)-based immunotherapy but are likely to have previously been identified in transcriptome or proteome analyses. Here, we show that the active conformer of an integrin can serve as a specific therapeutic target for multiple myeloma (MM). We screened >10,000 anti-MM mAb clones and identified MMG49 as an MM-specific mAb specifically recognizing a subset of integrin β7 molecules. The MMG49 epitope, in the N-terminal region of the β7 chain, is predicted to be inaccessible in the resting integrin conformer but exposed in the active conformation. Elevated expression and constitutive activation of integrin β7 conferred high MMG49 reactivity on MM cells, whereas MMG49 binding was scarcely detectable in other cell types including normal integrin β7+ lymphocytes. T cells transduced with MMG49-derived chimeric antigen receptor (CAR) exerted anti-MM effects without damaging normal hematopoietic cells. Thus, MMG49 CAR T cell therapy is promising for MM, and a receptor protein with a rare but physiologically relevant conformation can serve as a cancer immunotherapy target.", "gemini": "Các kháng nguyên bề mặt tế bào đặc hiệu của ung thư là mục tiêu lý tưởng cho liệu pháp miễn dịch dựa trên kháng thể đơn dòng (mAb) nhưng có khả năng đã được xác định trước đó trong các phân tích phiên mã hoặc proteome. Tại đây, chúng tôi chỉ ra rằng cấu tử hoạt động của integrin có thể đóng vai trò là mục tiêu điều trị đặc hiệu cho bệnh đa u tủy (MM). Chúng tôi đã sàng lọc >10.000 bản sao mAb kháng MM và xác định MMG49 là mAb đặc hiệu của MM nhận biết đặc hiệu một phân nhóm các phân tử integrin β7. Epitop MMG49, ở vùng đầu N của chuỗi β7, được dự đoán là không thể tiếp cận được ở cấu tử integrin nghỉ nhưng lại lộ ra ở cấu tử hoạt động. Biểu hiện tăng cao và hoạt hóa cấu thành của integrin β7 mang lại phản ứng MMG49 cao đối với các tế bào MM, trong khi liên kết MMG49 hầu như không phát hiện được ở các loại tế bào khác bao gồm cả tế bào lympho β7+ bình thường. Các tế bào T được chuyển đổi bằng thụ thể kháng nguyên chimeric có nguồn gốc từ MMG49 (CAR) phát huy tác dụng chống MM mà không gây tổn thương cho các tế bào tạo máu bình thường. Do đó, liệu pháp tế bào CAR T MMG49 rất hứa hẹn đối với MM và một protein thụ thể có cấu hình hiếm nhưng có liên quan đến sinh lý có thể đóng vai trò là mục tiêu của liệu pháp miễn dịch ung thư."} +{"idx": 1930120, "en": "Interferon regulatory factor 3 (IRF3) and type I interferons (IFNs) protect against infections and cancer, but excessive IRF3 activation and type I IFN production cause autoinflammatory conditions such as Aicardi-Goutières syndrome and STING-associated vasculopathy of infancy (SAVI). Myocardial infarction (MI) elicits inflammation, but the dominant molecular drivers of MI-associated inflammation remain unclear. Here we show that ischemic cell death and uptake of cell debris by macrophages in the heart fuel a fatal response to MI by activating IRF3 and type I IFN production. In mice, single-cell RNA-seq analysis of 4,215 leukocytes isolated from infarcted and non-infarcted hearts showed that MI provokes activation of an IRF3-interferon axis in a distinct population of interferon-inducible cells (IFNICs) that were classified as cardiac macrophages. Mice genetically deficient in cyclic GMP-AMP synthase (cGAS), its adaptor STING, IRF3, or the type I IFN receptor IFNAR exhibited impaired interferon-stimulated gene (ISG) expression and, in the case of mice deficient in IRF3 or IFNAR, improved survival after MI as compared to controls. Interruption of IRF3-dependent signaling resulted in decreased cardiac expression of inflammatory cytokines and chemokines and decreased inflammatory cell infiltration of the heart, as well as in attenuated ventricular dilation and improved cardiac function. Similarly, treatment of mice with an IFNAR-neutralizing antibody after MI ablated the interferon response and improved left ventricular dysfunction and survival. These results identify IRF3 and the type I IFN response as a potential therapeutic target for post-MI cardioprotection.", "gemini": "Yếu tố điều hòa interferon 3 (IRF3) và interferon loại I (IFN) bảo vệ chống lại nhiễm trùng và ung thư, nhưng hoạt hóa IRF3 quá mức và sản xuất IFN loại I gây ra các tình trạng tự miễn dịch như hội chứng Aicardi-Goutières và bệnh mạch máu liên quan đến STING ở trẻ sơ sinh (SAVI). Nhồi máu cơ tim (MI) gây ra tình trạng viêm, nhưng các yếu tố phân tử chính thúc đẩy tình trạng viêm liên quan đến MI vẫn chưa rõ ràng. Ở đây chúng tôi chỉ ra rằng chết tế bào do thiếu máu cục bộ và sự hấp thụ các mảnh vụn tế bào bởi các đại thực bào trong tim thúc đẩy phản ứng gây tử vong đối với MI bằng cách kích hoạt IRF3 và sản xuất IFN loại I. Ở chuột, phân tích RNA-seq tế bào đơn của 4.215 tế bào bạch cầu được phân lập từ tim nhồi máu và không nhồi máu cho thấy MI gây ra sự kích hoạt của trục IRF3-interferon trong một quần thể riêng biệt của các tế bào có thể cảm ứng interferon (IFNIC) được phân loại là đại thực bào tim. Chuột bị thiếu hụt gen tổng hợp GMP-AMP vòng (cGAS), bộ điều hợp STING, IRF3 hoặc thụ thể IFN loại I IFNAR biểu hiện gen kích thích interferon (ISG) bị suy yếu và trong trường hợp chuột thiếu IRF3 hoặc IFNAR, khả năng sống sót sau MI được cải thiện so với nhóm đối chứng. Ngắt tín hiệu phụ thuộc IRF3 dẫn đến giảm biểu hiện cytokine và chemokine gây viêm ở tim và giảm sự xâm nhập của tế bào viêm vào tim, cũng như làm giảm giãn thất và cải thiện chức năng tim. Tương tự, điều trị chuột bằng kháng thể trung hòa IFNAR sau MI đã xóa bỏ đáp ứng interferon và cải thiện chức năng thất trái và khả năng sống sót. Những kết quả này xác định IRF3 và đáp ứng IFN loại I là mục tiêu điều trị tiềm năng để bảo vệ tim sau MI."} +{"idx": 1930121, "en": "Sleep deprivation is a major source of morbidity with widespread health effects, including increased risk of hypertension, diabetes, obesity, heart attack, and stroke. Moreover, sleep deprivation brings about vehicle accidents and medical errors and is therefore an urgent topic of investigation. During sleep deprivation, homeostatic and circadian processes interact to build up sleep pressure, which results in slow behavioral performance (cognitive lapses) typically attributed to attentional thalamic and frontoparietal circuits, but the underlying mechanisms remain unclear. Recently, through study of electroencephalograms (EEGs) in humans and local field potentials (LFPs) in nonhuman primates and rodents it was found that, during sleep deprivation, regional 'sleep-like' slow and theta (slow/theta) waves co-occur with impaired behavioral performance during wakefulness. Here we used intracranial electrodes to record single-neuron activities and LFPs in human neurosurgical patients performing a face/nonface categorization psychomotor vigilance task (PVT) over multiple experimental sessions, including a session after full-night sleep deprivation. We find that, just before cognitive lapses, the selective spiking responses of individual neurons in the medial temporal lobe (MTL) are attenuated, delayed, and lengthened. These 'neuronal lapses' are evident on a trial-by-trial basis when comparing the slowest behavioral PVT reaction times to the fastest. Furthermore, during cognitive lapses, LFPs exhibit a relative local increase in slow/theta activity that is correlated with degraded single-neuron responses and with baseline theta activity. Our results show that cognitive lapses involve local state-dependent changes in neuronal activity already present in the MTL.", "gemini": "Mất ngủ là một nguồn gây bệnh chính với nhiều tác động đến sức khỏe, bao gồm tăng nguy cơ tăng huyết áp, tiểu đường, béo phì, đau tim và đột quỵ. Hơn nữa, mất ngủ gây ra tai nạn xe cộ và lỗi y khoa và do đó là một chủ đề cấp thiết cần điều tra. Trong quá trình mất ngủ, các quá trình cân bằng nội môi và nhịp sinh học tương tác để tạo ra áp lực ngủ, dẫn đến hiệu suất hành vi chậm (lỗi nhận thức) thường được cho là do các mạch thalamus chú ý và trán đỉnh, nhưng cơ chế cơ bản vẫn chưa rõ ràng. Gần đây, thông qua nghiên cứu điện não đồ (EEG) ở người và điện thế trường cục bộ (LFP) ở động vật linh trưởng không phải người và loài gặm nhấm, người ta thấy rằng trong quá trình mất ngủ, sóng chậm và sóng theta (chậm/theta) 'giống như giấc ngủ' khu trú cùng xuất hiện với hiệu suất hành vi bị suy giảm trong khi thức. Ở đây chúng tôi đã sử dụng các điện cực nội sọ để ghi lại các hoạt động của tế bào đơn và LFP ở những bệnh nhân phẫu thuật thần kinh ở người thực hiện nhiệm vụ cảnh giác tâm lý phân loại khuôn mặt/không phải khuôn mặt (PVT) trong nhiều phiên thực nghiệm, bao gồm một phiên sau khi mất ngủ cả đêm. Chúng tôi thấy rằng ngay trước khi xảy ra lỗi nhận thức, các phản ứng tăng đột biến có chọn lọc của các tế bào thần kinh riêng lẻ ở thùy thái dương giữa (MTL) bị suy yếu, chậm lại và kéo dài. Những 'lỗi thần kinh' này thể hiện rõ trên từng thử nghiệm khi so sánh thời gian phản ứng PVT hành vi chậm nhất với thời gian nhanh nhất. Hơn nữa, trong quá trình mất nhận thức, LFP biểu hiện sự gia tăng cục bộ tương đối ở hoạt động chậm/theta có liên quan đến các phản ứng tế bào thần kinh đơn bị suy giảm và hoạt động theta cơ bản. Kết quả của chúng tôi cho thấy lỗi nhận thức liên quan đến những thay đổi phụ thuộc vào trạng thái cục bộ trong hoạt động của tế bào thần kinh đã có trong MTL."} +{"idx": 1930122, "en": "Multiple adult tissues are maintained by stem cells of restricted developmental potential which can only form a subset of lineages within the tissue. For instance, the two adult lung epithelial compartments (airways and alveoli) are separately maintained by distinct lineage-restricted stem cells. A challenge has been to obtain multipotent stem cells and/or progenitors that can generate all epithelial cell types of a given tissue. Here we show that mouse Sox9+ multipotent embryonic lung progenitors can be isolated and expanded long term in 3D culture. Cultured Sox9+ progenitors transcriptionally resemble their in vivo counterparts and generate both airway and alveolar cell types in vitro. Sox9+ progenitors that were transplanted into injured adult mouse lungs differentiated into all major airway and alveolar lineages in vivo in a region-appropriate fashion. We propose that a single expandable embryonic lung progenitor population with broader developmental competence may eventually be used as an alternative for region-restricted adult tissue stem cells in regenerative medicine.", "gemini": "Nhiều mô ở người trưởng thành được duy trì bởi các tế bào gốc có tiềm năng phát triển hạn chế, chỉ có thể tạo thành một nhóm các dòng tế bào trong mô. Ví dụ, hai ngăn biểu mô phổi ở người trưởng thành (đường hô hấp và phế nang) được duy trì riêng biệt bởi các tế bào gốc hạn chế dòng tế bào riêng biệt. Một thách thức là thu được các tế bào gốc đa năng và/hoặc các tế bào tiền thân có thể tạo ra tất cả các loại tế bào biểu mô của một mô nhất định. Ở đây chúng tôi chỉ ra rằng các tiền thân phổi phôi đa năng Sox9+ của chuột có thể được phân lập và nhân rộng lâu dài trong nuôi cấy 3D. Các tiền thân Sox9+ được nuôi cấy có đặc điểm phiên mã giống với các tế bào tương ứng trong cơ thể sống và tạo ra cả các loại tế bào đường hô hấp và phế nang trong ống nghiệm. Các tiền thân Sox9+ được cấy ghép vào phổi chuột trưởng thành bị thương đã biệt hóa thành tất cả các dòng tế bào đường hô hấp và phế nang chính trong cơ thể sống theo cách phù hợp với vùng. Chúng tôi đề xuất rằng một quần thể tiền thân phổi phôi có thể mở rộng duy nhất với năng lực phát triển rộng hơn cuối cùng có thể được sử dụng làm phương án thay thế cho các tế bào gốc mô trưởng thành hạn chế vùng trong y học tái tạo."} +{"idx": 1930123, "en": "Eliminating errors in next-generation DNA sequencing has proved challenging. Here we present error-correction code (ECC) sequencing, a method to greatly improve sequencing accuracy by combining fluorogenic sequencing-by-synthesis (SBS) with an information theory-based error-correction algorithm. ECC embeds redundancy in sequencing reads by creating three orthogonal degenerate sequences, generated by alternate dual-base reactions. This is similar to encoding and decoding strategies that have proved effective in detecting and correcting errors in information communication and storage. We show that, when combined with a fluorogenic SBS chemistry with raw accuracy of 98.1%, ECC sequencing provides single-end, error-free sequences up to 200 bp. ECC approaches should enable accurate identification of extremely rare genomic variations in various applications in biology and medicine.", "gemini": "Việc loại bỏ lỗi trong giải trình tự DNA thế hệ tiếp theo đã được chứng minh là rất khó khăn. Ở đây chúng tôi trình bày giải trình tự mã sửa lỗi (ECC), một phương pháp cải thiện đáng kể độ chính xác của giải trình tự bằng cách kết hợp giải trình tự tổng hợp huỳnh quang (SBS) với thuật toán sửa lỗi dựa trên lý thuyết thông tin. ECC nhúng dự phòng vào các phép đọc giải trình tự bằng cách tạo ra ba trình tự suy biến trực giao, được tạo ra bởi các phản ứng hai bazơ xen kẽ. Điều này tương tự như các chiến lược mã hóa và giải mã đã chứng minh hiệu quả trong việc phát hiện và sửa lỗi trong truyền thông và lưu trữ thông tin. Chúng tôi chỉ ra rằng, khi kết hợp với hóa học SBS huỳnh quang có độ chính xác thô là 98,1%, giải trình tự ECC cung cấp các trình tự không lỗi, một đầu lên đến 200 bp. Các phương pháp ECC sẽ cho phép xác định chính xác các biến thể bộ gen cực kỳ hiếm trong các ứng dụng khác nhau trong sinh học và y học."} +{"idx": 1930124, "en": "Although advances in protein labeling methods have made it possible to measure the proteome of mixed cell populations, it has not been possible to isolate cell-type-specific proteomes in vivo. This is because the existing methods for metabolic protein labeling in vivo access all cell types. We report the development of a transgenic mouse line where Cre-recombinase-induced expression of a mutant methionyl-tRNA synthetase (L274G) enables the cell-type-specific labeling of nascent proteins with a non-canonical amino-acid and click chemistry. Using immunoblotting, imaging and mass spectrometry, we use our transgenic mouse to label and analyze proteins in excitatory principal neurons and Purkinje neurons in vitro (brain slices) and in vivo. We discover more than 200 proteins that are differentially regulated in hippocampal excitatory neurons by exposing mice to an environment with enriched sensory cues. Our approach can be used to isolate, analyze and quantitate cell-type-specific proteomes and their dynamics in healthy and diseased tissues.", "gemini": "Mặc dù những tiến bộ trong các phương pháp đánh dấu protein đã giúp đo lường được proteome của các quần thể tế bào hỗn hợp, nhưng vẫn chưa thể phân lập được các proteome đặc hiệu theo loại tế bào trong cơ thể sống. Điều này là do các phương pháp hiện có để đánh dấu protein chuyển hóa trong cơ thể sống tiếp cận được tất cả các loại tế bào. Chúng tôi báo cáo về sự phát triển của một dòng chuột chuyển gen, trong đó biểu hiện của synthetase methionyl-tRNA đột biến (L274G) do Cre-recombinase cảm ứng cho phép đánh dấu đặc hiệu theo loại tế bào các protein mới hình thành bằng một amino axit không điển hình và hóa học click. Sử dụng miễn dịch nhuộm, hình ảnh và phổ khối, chúng tôi sử dụng chuột chuyển gen của mình để đánh dấu và phân tích protein trong các tế bào thần kinh chính kích thích và tế bào thần kinh Purkinje trong ống nghiệm (lát não) và trong cơ thể sống. Chúng tôi phát hiện ra hơn 200 protein được điều chỉnh khác biệt ở các tế bào thần kinh kích thích vùng đồi hải mã bằng cách cho chuột tiếp xúc với môi trường có tín hiệu cảm giác phong phú. Phương pháp tiếp cận của chúng tôi có thể được sử dụng để phân lập, phân tích và định lượng các proteome đặc hiệu theo loại tế bào và động lực của chúng trong các mô khỏe mạnh và bệnh tật."} +{"idx": 1930125, "en": "Gene therapy may offer a new treatment option, particularly for patients with severe hemophilia, based on recent research. However, individuals with pre-existing immunity to adeno-associated viruses (AAVs) may be less likely to benefit from AAV vector-based therapies. To study pre-existing AAV5 immunity in humans, we validated two complementary, sensitive, and scalable in vitro assays to detect AAV5 total antibodies and transduction inhibition (TI). Using these two assays, we found that 53% of samples from 100 healthy male individuals were negative in both assays, 18% were positive in both assays, 5% were positive for total antibodies but negative for TI and, of interest, 24% were negative for total antibodies but positive for TI activity, suggesting the presence of non-antibody-based neutralizing factors in human plasma. Similar findings were obtained with 24 samples from individuals with hemophilia A. On the basis of these results, we describe the development of a dual-assay strategy to identify individuals without total AAV5 antibodies or neutralizing factors who may be more likely to respond to AAV5-directed gene therapy. These assays offer a universal, transferrable platform across laboratories to assess the global prevalence of AAV5 antibodies and neutralizing factors in large patient populations to help inform clinical development strategies.", "gemini": "Liệu pháp gen có thể cung cấp một lựa chọn điều trị mới, đặc biệt là đối với những bệnh nhân mắc bệnh ưa chảy máu nặng, dựa trên nghiên cứu gần đây. Tuy nhiên, những cá nhân có miễn dịch trước với vi-rút liên kết với adeno (AAV) có thể ít có khả năng hưởng lợi từ các liệu pháp dựa trên vectơ AAV. Để nghiên cứu miễn dịch AAV5 có từ trước ở người, chúng tôi đã xác nhận hai xét nghiệm trong ống nghiệm bổ sung, nhạy và có thể mở rộng quy mô để phát hiện tổng kháng thể AAV5 và ức chế chuyển nạp (TI). Sử dụng hai xét nghiệm này, chúng tôi thấy rằng 53% mẫu từ 100 cá thể nam khỏe mạnh có kết quả âm tính ở cả hai xét nghiệm, 18% có kết quả dương tính ở cả hai xét nghiệm, 5% có kết quả dương tính với tổng kháng thể nhưng âm tính với TI và, điều thú vị là 24% có kết quả âm tính với tổng kháng thể nhưng dương tính với hoạt động TI, cho thấy sự hiện diện của các yếu tố trung hòa không dựa trên kháng thể trong huyết tương người. Những phát hiện tương tự đã thu được với 24 mẫu từ những cá nhân mắc bệnh ưa chảy máu A. Trên cơ sở những kết quả này, chúng tôi mô tả sự phát triển của một chiến lược xét nghiệm kép để xác định những cá nhân không có tổng kháng thể AAV5 hoặc các yếu tố trung hòa, những người có khả năng đáp ứng với liệu pháp gen hướng đích AAV5 cao hơn. Các xét nghiệm này cung cấp một nền tảng phổ quát, có thể chuyển nhượng giữa các phòng thí nghiệm để đánh giá tỷ lệ lưu hành toàn cầu của kháng thể AAV5 và các yếu tố trung hòa trong quần thể bệnh nhân lớn để giúp đưa ra thông tin cho các chiến lược phát triển lâm sàng."} +{"idx": 1930126, "en": "DNA vaccines delivered using electroporation (EP) have had clinical success, but these EP methods generally utilize invasive needle electrodes. Here, we demonstrate the delivery and immunogenicity of a DNA vaccine into subcutaneous adipose tissue cells using noninvasive EP. Using finite element analysis, we predicted that plate electrodes, when oriented properly, could effectively concentrate the electric field within adipose tissue. In practice, these electrodes generated widespread gene expression persisting for at least 60 days in vivo within interscapular subcutaneous fat pads of guinea pigs. We then applied this adipose-EP protocol to deliver a DNA vaccine coding for an influenza antigen into guinea pigs. The resulting host immune responses elicited were of a similar magnitude to those achieved by skin delivery with EP. The onset of the humoral immune response was more rapid when the DNA dose was spread over multiple injection sites, and increasing the voltage of the EP device increased the magnitude of the immune response. This study supports further development of EP protocols delivering gene-based therapies to subcutaneous fat.", "gemini": "Các vắc-xin DNA được đưa vào bằng phương pháp điện di (EP) đã thành công trong lâm sàng, nhưng các phương pháp EP này thường sử dụng các điện cực kim xâm lấn. Ở đây, chúng tôi chứng minh việc đưa vào và khả năng sinh miễn dịch của vắc-xin DNA vào các tế bào mô mỡ dưới da bằng phương pháp điện di không xâm lấn. Sử dụng phân tích phần tử hữu hạn, chúng tôi dự đoán rằng các điện cực tấm, khi được định hướng đúng, có thể tập trung hiệu quả trường điện trong mô mỡ. Trong thực tế, các điện cực này tạo ra sự biểu hiện gen lan rộng kéo dài ít nhất 60 ngày trong cơ thể ở các miếng mỡ dưới da giữa hai xương bả vai của chuột lang. Sau đó, chúng tôi áp dụng giao thức điện di mỡ này để đưa vắc-xin DNA mã hóa cho một kháng nguyên cúm vào chuột lang. Phản ứng miễn dịch của vật chủ kết quả có cùng cường độ với phản ứng đạt được bằng cách đưa vào da bằng phương pháp điện di. Sự khởi phát của phản ứng miễn dịch dịch thể diễn ra nhanh hơn khi liều DNA được lan truyền trên nhiều vị trí tiêm và việc tăng điện áp của thiết bị điện di làm tăng cường độ của phản ứng miễn dịch. Nghiên cứu này hỗ trợ sự phát triển thêm các giao thức điện di đưa các liệu pháp dựa trên gen vào chất béo dưới da."} +{"idx": 1930127, "en": "Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional saturation mutagenesis approaches are labor intensive, and the resulting TCRs may be cross-reactive. Here we describe the in vitro maturation and selection of mouse and human T cells on antigen-expressing feeder cells to develop higher-affinity TCRs. The approach takes advantage of natural Tcrb gene rearrangement to generate diversity in the length and composition of CDR3β. In vitro differentiation of progenitors transduced with a known Tcra gene in the presence of antigen drives differentiation of cells with a distinct agonist-selected phenotype. We purified these cells to generate TCRβ chain libraries pre-enriched for target antigen specificity. Several TCRβ chains paired with a transgenic TCRα chain to produce a TCR with higher affinity than the parental TCR for target antigen, without evidence of cross-reactivity.", "gemini": "Nhiều mục tiêu đầy hứa hẹn cho các liệu pháp miễn dịch ung thư dựa trên tế bào T là các tự kháng nguyên. Trong quá trình chọn lọc ở tuyến ức, các tế bào T mang thụ thể tế bào T (TCR) có ái lực cao đối với tự kháng nguyên sẽ bị loại bỏ. Ái lực của các TCR có ái lực thấp còn lại có thể được cải thiện để tăng hiệu quả chống khối u của chúng, nhưng các phương pháp gây đột biến bão hòa thông thường rất tốn công và các TCR kết quả có thể phản ứng chéo. Ở đây, chúng tôi mô tả quá trình trưởng thành và lựa chọn trong ống nghiệm của các tế bào T của chuột và người trên các tế bào nuôi cấy biểu hiện kháng nguyên để phát triển các TCR có ái lực cao hơn. Phương pháp này tận dụng sự sắp xếp lại gen Tcrb tự nhiên để tạo ra sự đa dạng về chiều dài và thành phần của CDR3β. Sự biệt hóa trong ống nghiệm của các tế bào tiền thân được chuyển đổi với một gen Tcra đã biết khi có mặt kháng nguyên thúc đẩy sự biệt hóa của các tế bào có kiểu hình chọn chất chủ vận riêng biệt. Chúng tôi đã thanh lọc các tế bào này để tạo ra các thư viện chuỗi TCRβ được làm giàu trước cho tính đặc hiệu của kháng nguyên mục tiêu. Một số chuỗi TCRβ ghép nối với chuỗi TCRα chuyển gen để tạo ra TCR có ái lực cao hơn so với TCR gốc đối với kháng nguyên mục tiêu, mà không có bằng chứng về phản ứng chéo."} +{"idx": 1930128, "en": "Extrachromosomal telomere repeat (ECTR) DNA is unique to cancer cells that maintain telomeres through the alternative lengthening of telomeres (ALT) pathway, but the role of ECTRs in ALT development remains elusive. We found that induction of ECTRs in normal human fibroblasts activated the cGAS-STING-TBK1-IRF3 signaling axis to trigger IFNβ production and a type I interferon response, resulting in cell-proliferation defects. In contrast, ALT cancer cells are commonly defective in sensing cytosolic DNA. We found that STING expression was inhibited in ALT cancer cell lines and transformed ALT cells. Notably, the ALT suppressors histone H3.3 and the ATRX-Daxx histone chaperone complex were also required to activate the DNA-sensing pathway. Collectively, our data suggest that the loss of the cGAS-STING pathway may be required to evade ECTR-induced anti-proliferation effects and permit ALT development, and this requirement may be exploited for treatments specific to cancers utilizing the ALT pathway.", "gemini": "DNA lặp lại ngoại nhiễm sắc thể (ECTR) là duy nhất đối với các tế bào ung thư duy trì telomere thông qua con đường làm dài telomere thay thế (ALT), nhưng vai trò của ECTR trong sự phát triển của ALT vẫn còn khó nắm bắt. Chúng tôi phát hiện ra rằng việc gây ra ECTR ở nguyên bào sợi bình thường của người đã kích hoạt trục tín hiệu cGAS-STING-TBK1-IRF3 để kích hoạt sản xuất IFNβ và phản ứng interferon type I, dẫn đến khiếm khuyết về sự tăng sinh tế bào. Ngược lại, các tế bào ung thư ALT thường bị khiếm khuyết trong việc cảm nhận DNA trong tế bào chất. Chúng tôi phát hiện ra rằng biểu hiện STING bị ức chế ở các dòng tế bào ung thư ALT và các tế bào ALT đã chuyển đổi. Đáng chú ý, các chất ức chế ALT là histone H3.3 và phức hợp chaperone histone ATRX-Daxx cũng được yêu cầu để kích hoạt con đường cảm biến DNA. Nhìn chung, dữ liệu của chúng tôi cho thấy rằng sự mất đi của con đường cGAS-STING có thể là cần thiết để tránh các tác động chống tăng sinh do ECTR gây ra và cho phép sự phát triển của ALT, và yêu cầu này có thể được khai thác để điều trị đặc hiệu cho các bệnh ung thư sử dụng con đường ALT."} +{"idx": 1930129, "en": "Poly(A) tails are important elements in mRNA translation and stability, although recent genome-wide studies have concluded that poly(A) tail length is generally not associated with translational efficiency in nonembryonic cells. To investigate whether poly(A) tail size might be coupled to gene expression in an intact organism, we used an adapted TAIL-seq protocol to measure poly(A) tails in Caenorhabditis elegans. Surprisingly, we found that well-expressed transcripts contain relatively short, well-defined tails. This attribute appears to be dependent on translational efficiency, as transcripts enriched for optimal codons and ribosome association had the shortest tail sizes, whereas noncoding RNAs retained long tails. Across eukaryotes, short tails were a feature of abundant and well-translated mRNAs. This seems to contradict the dogma that deadenylation induces translational inhibition and mRNA decay and suggests that well-expressed mRNAs accumulate with pruned tails that accommodate a minimal number of poly(A)-binding proteins, which may be ideal for protective and translational functions.", "gemini": "Đuôi poly(A) là những thành phần quan trọng trong quá trình dịch mã và ổn định mRNA, mặc dù các nghiên cứu trên toàn bộ hệ gen gần đây đã kết luận rằng chiều dài đuôi poly(A) thường không liên quan đến hiệu quả dịch mã ở các tế bào không phải phôi. Để điều tra xem kích thước đuôi poly(A) có thể được ghép nối với biểu hiện gen trong một cơ thể nguyên vẹn hay không, chúng tôi đã sử dụng một giao thức TAIL-seq được điều chỉnh để đo đuôi poly(A) ở Caenorhabditis elegans. Đáng ngạc nhiên, chúng tôi thấy rằng các bản sao được biểu hiện tốt có đuôi tương đối ngắn và được xác định rõ. Thuộc tính này dường như phụ thuộc vào hiệu quả dịch mã, vì các bản sao được làm giàu cho các codon tối ưu và sự liên kết với ribosome có kích thước đuôi ngắn nhất, trong khi các RNA không mã hóa vẫn giữ được đuôi dài. Trên khắp các sinh vật nhân chuẩn, đuôi ngắn là một đặc điểm của mRNA dồi dào và được dịch tốt. Điều này dường như mâu thuẫn với giáo điều cho rằng sự chết tiệt gây ra sự ức chế dịch mã và sự phân hủy mRNA và cho thấy rằng các mRNA được biểu hiện tốt tích tụ với các đuôi được cắt tỉa để chứa một số lượng tối thiểu các protein liên kết poly(A), có thể lý tưởng cho các chức năng bảo vệ và dịch mã."} +{"idx": 1930130, "en": "Eukaryotic mRNA transcription initiation is directed by the formation of the megadalton-sized preinitiation complex (PIC). After PIC formation, double-stranded DNA (dsDNA) is unwound to form a single-stranded DNA bubble, and the template strand is loaded into the polymerase active site. DNA opening is catalyzed by Ssl2 (XPB), the dsDNA translocase subunit of the basal transcription factor TFIIH. In yeast, transcription initiation proceeds through a scanning phase during which downstream DNA is searched for optimal start sites. Here, to test models for initial DNA opening and start-site scanning, we measure the DNA-bubble sizes generated by Saccharomyces cerevisiae PICs in real time using single-molecule magnetic tweezers. We show that ATP hydrolysis by Ssl2 opens a 6-base-pair (bp) bubble that grows to 13 bp in the presence of NTPs. These observations support a two-step model wherein ATP-dependent Ssl2 translocation leads to a 6-bp open complex that RNA polymerase II expands via NTP-dependent RNA transcription.", "gemini": "Khởi đầu phiên mã mRNA của sinh vật nhân chuẩn được chỉ đạo bởi sự hình thành của phức hợp tiền khởi đầu (PIC) có kích thước megadalton. Sau khi hình thành PIC, DNA hai sợi (dsDNA) được tháo xoắn để tạo thành một bong bóng DNA một sợi và sợi khuôn được đưa vào vị trí hoạt động của polymerase. Quá trình mở DNA được xúc tác bởi Ssl2 (XPB), tiểu đơn vị chuyển vị dsDNA của yếu tố phiên mã cơ bản TFIIH. Ở nấm men, khởi đầu phiên mã diễn ra thông qua một giai đoạn quét trong đó tìm kiếm các vị trí bắt đầu tối ưu cho DNA hạ nguồn. Tại đây, để kiểm tra các mô hình mở DNA ban đầu và quét vị trí bắt đầu, chúng tôi đo kích thước bong bóng DNA do các PIC của Saccharomyces cerevisiae tạo ra theo thời gian thực bằng cách sử dụng nhíp từ đơn phân tử. Chúng tôi chỉ ra rằng thủy phân ATP bởi Ssl2 mở ra một bong bóng 6 cặp bazơ (bp) phát triển thành 13 bp khi có mặt NTP. Những quan sát này hỗ trợ một mô hình hai bước trong đó sự dịch chuyển Ssl2 phụ thuộc ATP dẫn đến một phức hợp mở 6 bp mà RNA polymerase II mở rộng thông qua phiên mã RNA phụ thuộc NTP."} +{"idx": 1930131, "en": "TRPML3 channels are mainly localized to endolysosomes and play a critical role in the endocytic pathway. Their dysfunction causes deafness and pigmentation defects in mice. TRPML3 activity is inhibited by low endolysosomal pH. Here we present cryo-electron microscopy (cryo-EM) structures of human TRPML3 in the closed, agonist-activated, and low-pH-inhibited states, with resolutions of 4.06, 3.62, and 4.65 Å, respectively. The agonist ML-SA1 lodges between S5 and S6 and opens an S6 gate. A polycystin-mucolipin domain (PMD) forms a luminal cap. S1 extends into this cap, forming a 'gating rod' that connects directly to a luminal pore loop, which undergoes dramatic conformational changes in response to low pH. S2 extends intracellularly and interacts with several intracellular regions to form a 'gating knob'. These unique structural features, combined with the results of electrophysiological studies, indicate a new mechanism by which luminal pH and other physiological modulators such as PIP2 regulate TRPML3 by changing S1 and S2 conformations.", "gemini": "Các kênh TRPML3 chủ yếu tập trung ở các nội thể và đóng vai trò quan trọng trong con đường nội bào. Rối loạn chức năng của chúng gây ra điếc và khiếm khuyết sắc tố ở chuột. Hoạt động của TRPML3 bị ức chế bởi độ pH nội thể thấp. Tại đây, chúng tôi trình bày các cấu trúc kính hiển vi điện tử lạnh (cryo-EM) của TRPML3 của người ở trạng thái đóng, hoạt hóa bởi chất chủ vận và ức chế ở pH thấp, với độ phân giải lần lượt là 4,06, 3,62 và 4,65 Å. Chất chủ vận ML-SA1 nằm giữa S5 và S6 và mở một cổng S6. Một miền polycystin-mucolipin (PMD) tạo thành một nắp nội bào. S1 mở rộng vào nắp này, tạo thành một 'thanh đóng' kết nối trực tiếp với một vòng lặp lỗ nội bào, trải qua những thay đổi cấu hình mạnh mẽ để đáp ứng với độ pH thấp. S2 mở rộng vào trong tế bào và tương tác với một số vùng nội bào để tạo thành một 'núm đóng'. Các đặc điểm cấu trúc độc đáo này, kết hợp với kết quả của các nghiên cứu điện sinh lý, chỉ ra một cơ chế mới mà theo đó độ pH nội bào và các chất điều hòa sinh lý khác như PIP2 điều chỉnh TRPML3 bằng cách thay đổi cấu hình S1 và S2."} +{"idx": 1930132, "en": "A widespread approach to modern cancer therapy is to identify a single oncogenic driver gene and target its mutant-protein product (for example, EGFR-inhibitor treatment in EGFR-mutant lung cancers). However, genetically driven resistance to targeted therapy limits patient survival. Through genomic analysis of 1,122 EGFR-mutant lung cancer cell-free DNA samples and whole-exome analysis of seven longitudinally collected tumor samples from a patient with EGFR-mutant lung cancer, we identified critical co-occurring oncogenic events present in most advanced-stage EGFR-mutant lung cancers. We defined new pathways limiting EGFR-inhibitor response, including WNT/β-catenin alterations and cell-cycle-gene (CDK4 and CDK6) mutations. Tumor genomic complexity increases with EGFR-inhibitor treatment, and co-occurring alterations in CTNNB1 and PIK3CA exhibit nonredundant functions that cooperatively promote tumor metastasis or limit EGFR-inhibitor response. This study calls for revisiting the prevailing single-gene driver-oncogene view and links clinical outcomes to co-occurring genetic alterations in patients with advanced-stage EGFR-mutant lung cancer.", "gemini": "Một cách tiếp cận rộng rãi đối với liệu pháp ung thư hiện đại là xác định một gen điều khiển ung thư đơn lẻ và nhắm mục tiêu vào sản phẩm protein đột biến của nó (ví dụ: điều trị bằng thuốc ức chế EGFR ở ung thư phổi đột biến EGFR). Tuy nhiên, tình trạng kháng thuốc do di truyền đối với liệu pháp nhắm mục tiêu hạn chế sự sống còn của bệnh nhân. Thông qua phân tích bộ gen của 1.122 mẫu DNA không tế bào ung thư phổi đột biến EGFR và phân tích toàn bộ exome của bảy mẫu khối u được thu thập theo chiều dọc từ một bệnh nhân ung thư phổi đột biến EGFR, chúng tôi đã xác định các sự kiện ung thư đồng thời quan trọng có mặt ở hầu hết các loại ung thư phổi đột biến EGFR giai đoạn tiến triển. Chúng tôi đã xác định các con đường mới hạn chế đáp ứng với thuốc ức chế EGFR, bao gồm các biến đổi WNT/β-catenin và đột biến gen chu kỳ tế bào (CDK4 và CDK6). Độ phức tạp của bộ gen khối u tăng lên khi điều trị bằng thuốc ức chế EGFR và các biến đổi đồng thời ở CTNNB1 và PIK3CA thể hiện các chức năng không trùng lặp, cùng nhau thúc đẩy di căn khối u hoặc hạn chế đáp ứng với thuốc ức chế EGFR. Nghiên cứu này kêu gọi xem xét lại quan điểm về gen điều khiển ung thư đơn gen hiện hành và liên kết kết quả lâm sàng với các biến đổi di truyền đồng thời ở những bệnh nhân ung thư phổi đột biến EGFR giai đoạn tiến triển."} +{"idx": 1930133, "en": "Cancer genomics efforts have identified genes and regulatory elements driving cancer development and neoplastic progression. From a microevolution standpoint, these are subject to positive selection. Although elusive in current studies, genes whose wild-type coding sequences are needed for tumor growth are also of key interest. They are expected to experience negative selection and stay intact under pressure of incessant mutation. The detection of significantly mutated (or undermutated) genes is completely confounded by the genomic heterogeneity of cancer mutation. Here we present a hierarchical framework that allows modeling of coding point mutations. Application of the model to sequencing data from 17 cancer types demonstrates an increased power to detect known cancer driver genes and identifies new significantly mutated genes with highly plausible biological functions. The signal of negative selection is very subtle, but is detectable in several cancer types and in a pan-cancer data set. It is enriched in cell-essential genes identified in a CRISPR screen, as well as in genes with reported roles in cancer.", "gemini": "Những nỗ lực về bộ gen ung thư đã xác định các gen và các yếu tố điều hòa thúc đẩy sự phát triển của ung thư và sự tiến triển của khối u. Về mặt tiến hóa vi mô, đây là những đối tượng chịu sự chọn lọc tích cực. Mặc dù khó nắm bắt trong các nghiên cứu hiện tại, các gen có trình tự mã hóa kiểu hoang dã cần thiết cho sự phát triển của khối u cũng rất được quan tâm. Chúng dự kiến sẽ trải qua quá trình chọn lọc tiêu cực và vẫn còn nguyên vẹn dưới áp lực của đột biến liên tục. Việc phát hiện các gen đột biến đáng kể (hoặc đột biến thấp) hoàn toàn bị ảnh hưởng bởi tính không đồng nhất của bộ gen trong đột biến ung thư. Ở đây chúng tôi trình bày một khuôn khổ phân cấp cho phép lập mô hình các đột biến điểm mã hóa. Việc áp dụng mô hình vào dữ liệu giải trình tự từ 17 loại ung thư cho thấy khả năng phát hiện các gen điều khiển ung thư đã biết được tăng lên và xác định các gen đột biến đáng kể mới có chức năng sinh học rất hợp lý. Tín hiệu của sự chọn lọc tiêu cực rất tinh tế, nhưng có thể phát hiện thấy ở một số loại ung thư và trong một tập dữ liệu toàn ung thư. Nó được làm giàu trong các gen thiết yếu của tế bào được xác định trong sàng lọc CRISPR, cũng như trong các gen có vai trò được báo cáo trong ung thư."} +{"idx": 1930134, "en": "Accurate annotation of genes and their transcripts is a foundation of genomics, but currently no annotation technique combines throughput and accuracy. As a result, reference gene collections remain incomplete-many gene models are fragmentary, and thousands more remain uncataloged, particularly for long noncoding RNAs (lncRNAs). To accelerate lncRNA annotation, the GENCODE consortium has developed RNA Capture Long Seq (CLS), which combines targeted RNA capture with third-generation long-read sequencing. Here we present an experimental reannotation of the GENCODE intergenic lncRNA populations in matched human and mouse tissues that resulted in novel transcript models for 3,574 and 561 gene loci, respectively. CLS approximately doubled the annotated complexity of targeted loci, outperforming existing short-read techniques. Full-length transcript models produced by CLS enabled us to definitively characterize the genomic features of lncRNAs, including promoter and gene structure, and protein-coding potential. Thus, CLS removes a long-standing bottleneck in transcriptome annotation and generates manual-quality full-length transcript models at high-throughput scales.", "gemini": "Chú thích chính xác các gen và bản sao của chúng là nền tảng của bộ gen, nhưng hiện tại không có kỹ thuật chú thích nào kết hợp được thông lượng và độ chính xác. Do đó, các bộ sưu tập gen tham chiếu vẫn còn thiếu sót - nhiều mô hình gen còn rời rạc và hàng nghìn mô hình khác vẫn chưa được lập danh mục, đặc biệt là đối với RNA không mã hóa dài (lncRNA). Để đẩy nhanh quá trình chú thích lncRNA, nhóm nghiên cứu GENCODE đã phát triển RNA Capture Long Seq (CLS), kết hợp việc bắt giữ RNA mục tiêu với giải trình tự đọc dài thế hệ thứ ba. Ở đây chúng tôi trình bày một chú thích lại thực nghiệm về quần thể lncRNA giữa các gen của GENCODE trong các mô của người và chuột được ghép nối, dẫn đến các mô hình bản sao mới cho 3.574 và 561 locus gen, tương ứng. CLS tăng gấp đôi độ phức tạp được chú thích của các locus mục tiêu, vượt trội hơn các kỹ thuật đọc ngắn hiện có. Các mô hình bản sao toàn bộ được tạo ra bởi CLS cho phép chúng tôi đặc trưng dứt khoát các đặc điểm bộ gen của lncRNA, bao gồm cấu trúc gen và vùng khởi động, và tiềm năng mã hóa protein. Do đó, CLS loại bỏ một nút thắt cổ chai lâu dài trong chú thích phiên mã và tạo ra các mô hình bản sao toàn bộ chất lượng thủ công ở quy mô thông lượng cao."} +{"idx": 1930135, "en": "While recent studies have identified higher than anticipated heterogeneity of mutation rate across genomic regions, mutations in exons and introns are assumed to be generated at the same rate. Here we find fewer somatic mutations in exons than expected from their sequence content and demonstrate that this is not due to purifying selection. Instead, we show that it is caused by higher mismatch-repair activity in exonic than in intronic regions. Our findings have important implications for understanding of mutational and DNA repair processes and knowledge of the evolution of eukaryotic genes, and they have practical ramifications for the study of evolution of both tumors and species.", "gemini": "Mặc dù các nghiên cứu gần đây đã xác định tỷ lệ đột biến không đồng nhất cao hơn dự kiến trên các vùng bộ gen, nhưng người ta cho rằng các đột biến ở exon và intron được tạo ra ở cùng một tỷ lệ. Ở đây chúng tôi tìm thấy ít đột biến soma ở exon hơn dự kiến từ nội dung trình tự của chúng và chứng minh rằng điều này không phải do quá trình chọn lọc thanh lọc. Thay vào đó, chúng tôi chỉ ra rằng nó là do hoạt động sửa chữa sai sót cao hơn ở vùng ngoại lai so với vùng intron. Những phát hiện của chúng tôi có ý nghĩa quan trọng để hiểu các quá trình đột biến và sửa chữa DNA cũng như kiến thức về sự tiến hóa của các gen nhân chuẩn và chúng có ý nghĩa thực tế đối với nghiên cứu về sự tiến hóa của cả khối u và loài."} +{"idx": 1930136, "en": "XtalFluor-E® has been extensively used in a broad range of reactions in the past few years. Here we report its use with protic nucleophiles in a catalytic manner for the in situ generation of protons that lead to the proto-functionalization of activated olefins. Utilizing the latter protocol, proto etherification of enamides gives rise to N,O-acetals in nearly quantitative yields.", "gemini": "XtalFluor-E® đã được sử dụng rộng rãi trong nhiều phản ứng khác nhau trong vài năm qua. Ở đây chúng tôi báo cáo việc sử dụng nó với các chất ái nhân tử protic theo cách xúc tác để tạo ra các proton tại chỗ dẫn đến chức năng hóa nguyên mẫu của các olefin được hoạt hóa. Sử dụng giao thức sau, quá trình ether hóa nguyên mẫu của enamide tạo ra N, O-acetal với hiệu suất gần như định lượng."} +{"idx": 1930137, "en": "A high-performance actuator should be able to deliver large-shape deformations, fast actuations and sensitive responses to multiple stimuli. Here, we report such an actuator constructed from one layer of polyvinylidene fluoride (PVDF) with a high coefficient of thermal expansion (CTE), and another layer of small sheets of graphene oxide (SGO) with a negative CTE. The opposite deformations of both actuation layers make the SGO/PVDF bilayer actuator highly sensitive to the temperature stimulus with a large bending sensitivity of 1.5 cm-1 °C-1. Upon irradiation with 60 mW cm-2 infrared light, this SGO/PVDF bilayer actuator displayed an extremely rapid tip displacement rate of 140 mm s-1. Furthermore, this actuator can also sensitively respond to moisture because of its SGO layer, showing a curvature change from -22 to 13 cm-1 upon changing the relative humidity (RH) from 11% to 86%. This actuator can generate a contractile or relaxed stress 18 times that of mammalian skeletal muscle, under light irradiation or moisture with a response time as short as 1 s, being capable of lifting an object with a weight 80 times that of itself. Furthermore, it also showed excellent stability and repeatability.", "gemini": "Một bộ truyền động hiệu suất cao phải có khả năng tạo ra các biến dạng hình dạng lớn, hoạt động nhanh và phản ứng nhạy với nhiều kích thích. Ở đây, chúng tôi báo cáo một bộ truyền động như vậy được chế tạo từ một lớp polyvinylidene fluoride (PVDF) với hệ số giãn nở nhiệt (CTE) cao và một lớp khác gồm các tấm nhỏ graphene oxide (SGO) có CTE âm. Các biến dạng ngược nhau của cả hai lớp truyền động làm cho bộ truyền động hai lớp SGO/PVDF rất nhạy với kích thích nhiệt độ với độ nhạy uốn lớn là 1,5 cm-1 °C-1. Khi chiếu sáng bằng ánh sáng hồng ngoại 60 mW cm-2, bộ truyền động hai lớp SGO/PVDF này hiển thị tốc độ dịch chuyển đầu cực nhanh là 140 mm s-1. Hơn nữa, bộ truyền động này cũng có thể phản ứng nhạy với độ ẩm do có lớp SGO, cho thấy độ cong thay đổi từ -22 đến 13 cm-1 khi thay đổi độ ẩm tương đối (RH) từ 11% đến 86%. Bộ truyền động này có thể tạo ra ứng suất co hoặc giãn gấp 18 lần so với cơ xương của động vật có vú, dưới sự chiếu sáng hoặc độ ẩm với thời gian phản ứng chỉ trong 1 giây, có khả năng nâng vật có trọng lượng gấp 80 lần trọng lượng của chính nó. Hơn nữa, nó cũng cho thấy độ ổn định và khả năng lặp lại tuyệt vời."} +{"idx": 1930138, "en": "Chemical exfoliation of molybdenum disulfide (2H-MoS2) for preparing high-yield single-layer sheets has attracted considerable attention in recent years. However, the stability and nature of the resulting nanosheets are poorly understood. Storing the dispersion in ambient air brings about the reoxidation of the nanosheets, releasing their residual negative charges into the environment. The reoxidation facilitates lateral fractures and destabilizes the dispersion. In-plane X-ray diffraction of the nanosheets indicates that they have a 1T structure with a 2D √3 × 1 rectangular cell as the intrinsic structure for chemically exfoliated MoS2. We found that the 1T structure was preserved after reoxidation upon aging the dispersions in air, suggesting the formation of metastable neutral MoS2. The changes in the chemical nature of the nanosheets can be monitored by X-ray diffraction of the restacked nanosheets. The restacked nanosheets, obtained by drying the freshly prepared dispersion, exhibited an expanded bilayer hydrate structure, accommodating Li ions. On the other hand, dried samples from the aged dispersions were substantially composed of a deintercalated phase and the bilayer hydrate. Upon prolonged aging, the former phase became predominant with total disappearance of the latter. This evolution suggests that the reoxidation occurred sheet by sheet with a direct restoration of the original oxidation states of the nanosheets, whereas the oxidation states of the nanosheets can be discrete at 4+ and (4 - δ)+.", "gemini": "Việc tẩy lớp hóa học của molypden disulfide (2H-MoS2) để chế tạo các tấm đơn lớp có năng suất cao đã thu hút được nhiều sự chú ý trong những năm gần đây. Tuy nhiên, tính ổn định và bản chất của các nanosheet thu được vẫn chưa được hiểu rõ. Việc lưu trữ phân tán trong không khí xung quanh sẽ làm nanosheet bị oxy hóa trở lại, giải phóng các điện tích âm còn lại của chúng vào môi trường. Sự oxy hóa trở lại tạo điều kiện cho các vết nứt bên và làm mất ổn định sự phân tán. Phép nhiễu xạ tia X trong mặt phẳng của các nanosheet chỉ ra rằng chúng có cấu trúc 1T với ô hình chữ nhật √3 × 1 2D làm cấu trúc nội tại cho MoS2 được tẩy lớp hóa học. Chúng tôi phát hiện ra rằng cấu trúc 1T được bảo toàn sau khi oxy hóa trở lại khi để các phân tán già đi trong không khí, cho thấy sự hình thành của MoS2 trung tính không bền. Có thể theo dõi các thay đổi về bản chất hóa học của các nanosheet bằng cách nhiễu xạ tia X các nanosheet được xếp chồng lại. Các nanosheet được xếp chồng lại, thu được bằng cách làm khô phân tán mới được chuẩn bị, biểu hiện cấu trúc hydrat lớp kép mở rộng, chứa các ion Li. Mặt khác, các mẫu khô từ các phân tán đã già đi về cơ bản bao gồm một pha xen kẽ và hydrat lớp kép. Sau thời gian già hóa kéo dài, pha trước trở nên chiếm ưu thế với sự biến mất hoàn toàn của pha sau. Sự tiến hóa này cho thấy rằng sự oxy hóa trở lại xảy ra từng lớp một với sự phục hồi trực tiếp trạng thái oxy hóa ban đầu của các nanosheet, trong khi trạng thái oxy hóa của các nanosheet có thể rời rạc ở mức 4+ và (4 - δ)+."} +{"idx": 1930139, "en": "Electron localisation function (ELF) calculations have been used to provide the first computational location of the cation lone pairs in apatite materials. We show that the orientation of the lone pairs varies depending on the identity and positions of the channel anions. The results represent a new platform for interpreting experimentally observed structure-property relationships in functional apatites. In particular, they have significant implications for ionic conductivity and suggest that structure-property relationships in lone-pair containing apatite-type solid electrolytes are more complex than previously thought.", "gemini": "Các phép tính hàm cục bộ hóa electron (ELF) đã được sử dụng để cung cấp vị trí tính toán đầu tiên của các cặp đơn cation trong vật liệu apatit. Chúng tôi chỉ ra rằng hướng của các cặp đơn thay đổi tùy thuộc vào danh tính và vị trí của các anion kênh. Các kết quả đại diện cho một nền tảng mới để giải thích các mối quan hệ cấu trúc-tính chất quan sát được trong các apatit chức năng. Đặc biệt, chúng có ý nghĩa quan trọng đối với độ dẫn ion và cho thấy rằng các mối quan hệ cấu trúc-tính chất trong các chất điện phân rắn loại apatit chứa cặp đơn phức tạp hơn so với suy nghĩ trước đây."} +{"idx": 1930140, "en": "We present herein the Pt(ii)-directed self-assembly in water of a new conformationally flexible N-monoalkyl-4,4-bipyridinium-based ditopic ligand into a library of six different metallacyclic structures. This constitutionally dynamic library can be pushed to increase the production of one of the supramolecules in aqueous media, either by controlling the concentration of the building blocks or upon addition of an appropriate aromatic substrate.", "gemini": "Chúng tôi trình bày ở đây quá trình tự lắp ráp do Pt(ii) hướng dẫn trong nước của một phối tử ditopic dựa trên N-monoalkyl-4,4-bipyridinium có cấu hình linh hoạt mới thành một thư viện gồm sáu cấu trúc kim loại khác nhau. Thư viện động về mặt cấu tạo này có thể được thúc đẩy để tăng sản xuất một trong các siêu phân tử trong môi trường nước, bằng cách kiểm soát nồng độ của các khối xây dựng hoặc khi bổ sung một chất nền thơm thích hợp."} +{"idx": 1930141, "en": "The nature of the initial structural events during the base-induced unfolding of the native (N) state of proteins is poorly understood. Combining site-specific fluorescence resonance energy transfer, size exclusion chromatography, dynamic fluorescence quenching, red-edge excitation shift and circular dichroism spectroscopy, we show here that an early intermediate during the base-induced unfolding of a multidomain protein, i.e., the B form, has features of a dry molten globule. We show that the N ⇌ B transition involves protein expansion and loosening of packing of inter-domain helices near domains I and II without the disruption of intra-domain packing or any change in hydration of the inter-domain region which resembles a molten hydrocarbon. Surprisingly, the disruption of inter-domain packing accounts for 40-45% of the total change in free energy of complete unfolding. Our results show that the disruption of van der Waals packing can be decoupled in different regions of a protein and could occur prior to hydrophobic solvation during base-induced unfolding, challenging the existing notion.", "gemini": "Bản chất của các sự kiện cấu trúc ban đầu trong quá trình mở ra do bazơ gây ra của trạng thái gốc (N) của protein vẫn chưa được hiểu rõ. Kết hợp truyền năng lượng cộng hưởng huỳnh quang tại vị trí cụ thể, sắc ký loại trừ kích thước, giải hấp huỳnh quang động, dịch chuyển kích thích cạnh đỏ và quang phổ lưỡng sắc tròn, chúng tôi chỉ ra ở đây rằng một chất trung gian sớm trong quá trình mở ra do bazơ gây ra của một protein đa miền, tức là dạng B, có các đặc điểm của một cầu tan khô. Chúng tôi chỉ ra rằng quá trình chuyển đổi N ⇌ B liên quan đến sự giãn nở protein và nới lỏng sự đóng gói của các xoắn liên miền gần miền I và II mà không phá vỡ sự đóng gói trong miền hoặc bất kỳ thay đổi nào về hydrat hóa của vùng liên miền giống như một hydrocarbon nóng chảy. Đáng ngạc nhiên, sự phá vỡ sự đóng gói liên miền chiếm 40-45% tổng thay đổi năng lượng tự do của quá trình mở ra hoàn toàn. Kết quả của chúng tôi cho thấy rằng sự phá vỡ sự đóng gói van der Waals có thể bị tách rời ở các vùng khác nhau của protein và có thể xảy ra trước quá trình hòa tan kỵ nước trong quá trình mở ra do bazơ gây ra, thách thức quan niệm hiện tại."} +{"idx": 1930142, "en": "A siloxane-based hexacarboxylic acid (L1-H6) has been prepared and applied in MOF construction. L1-H6 itself crystallizes as an unusual interpenetrated 3D hydrogen-bonded framework. Reaction of L1-H6 with Zn(ii) gave IMP-18 - a 3D MOF incorporating Si-O-Si functionality. Cleavage of L1-H6 gives a silanol-based triacid which is shown to give a coordination polymer (IMP-19) with Zn(ii).", "gemini": "Một axit hexacacboxylic gốc siloxan (L1-H6) đã được điều chế và ứng dụng trong chế tạo MOF. Bản thân L1-H6 kết tinh như một khung liên kết hydro 3D xen kẽ bất thường. Phản ứng của L1-H6 với Zn(ii) tạo ra IMP-18 - một MOF 3D kết hợp chức năng Si-O-Si. Phân cắt L1-H6 tạo ra một triaxit gốc silanol, được chứng minh là tạo ra một polyme phối hợp (IMP-19) với Zn(ii)."} +{"idx": 1930143, "en": "Nonalcoholic fatty liver disease (NAFLD) is the main cause of chronic liver disease worldwide. Previous evidence indicates that probiotics can be applied as a therapeutic agent for NAFLD. In this study, the potential probiotic strain Lactobacillus paracasei Jlus66 was isolated from natural fermented milk by a culture-dependent method, and its probiotic potentials were tested by established in vitro tests. In addition, the protective effect of Lactobacillus paracasei Jlus66 against NAFLD was evaluated in rat models. Compared with the high-fat-diet (HFD) group, the rats administered with 4 × 1010 cfu Jlus66 had significantly lower body weight gain, serum triglyceride (TG), low-density lipoprotein (LDL) as well as aminotransferase (ALT). Histopathological analysis showed Jlus66 also reduced the level of hepatic triglycerides and steatosis. From the above we conclude that L. paracasei Jlus66 has great potential as a probiotic in protecting from NAFLD.", "gemini": "Bệnh gan nhiễm mỡ không do rượu (NAFLD) là nguyên nhân chính gây bệnh gan mãn tính trên toàn thế giới. Các bằng chứng trước đây chỉ ra rằng chế phẩm sinh học có thể được sử dụng như một tác nhân điều trị NAFLD. Trong nghiên cứu này, chủng probiotic Lactobacillus paracasei Jlus66 tiềm năng đã được phân lập từ sữa lên men tự nhiên bằng phương pháp nuôi cấy phụ thuộc và các tiềm năng probiotic của nó đã được thử nghiệm bằng các xét nghiệm in vitro đã thiết lập. Ngoài ra, tác dụng bảo vệ của Lactobacillus paracasei Jlus66 chống lại NAFLD đã được đánh giá trên mô hình chuột. So với nhóm ăn nhiều chất béo (HFD), những con chuột được dùng 4 × 1010 cfu Jlus66 có mức tăng cân, triglycerid huyết thanh (TG), lipoprotein tỷ trọng thấp (LDL) cũng như aminotransferase (ALT) thấp hơn đáng kể. Phân tích mô bệnh học cho thấy Jlus66 cũng làm giảm mức triglycerid gan và chứng gan nhiễm mỡ. Từ những điều trên, chúng tôi kết luận rằng L. paracasei Jlus66 có tiềm năng lớn như một chế phẩm sinh học trong việc bảo vệ khỏi NAFLD."} diff --git a/retranslated_vi_pubmed/chunk_193/error.json b/retranslated_vi_pubmed/chunk_193/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_193/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_194/completed.json b/retranslated_vi_pubmed/chunk_194/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..2e6a62eb76af2efd509edf8165b8b27a159cafd7 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_194/completed.json @@ -0,0 +1,146 @@ +[ + 1940000, + 1940001, + 1940002, + 1940003, + 1940004, + 1940005, + 1940006, + 1940007, + 1940008, + 1940009, + 1940010, + 1940011, + 1940012, + 1940013, + 1940014, + 1940015, + 1940016, + 1940017, + 1940018, + 1940019, + 1940020, + 1940021, + 1940022, + 1940023, + 1940024, + 1940025, + 1940026, + 1940027, + 1940028, + 1940029, + 1940030, + 1940031, + 1940032, + 1940033, + 1940034, + 1940035, + 1940036, + 1940037, + 1940038, + 1940039, + 1940040, + 1940041, + 1940042, + 1940043, + 1940044, + 1940045, + 1940046, + 1940047, + 1940048, + 1940049, + 1940050, + 1940051, + 1940052, + 1940053, + 1940054, + 1940055, + 1940056, + 1940057, + 1940058, + 1940059, + 1940060, + 1940061, + 1940062, + 1940063, + 1940064, + 1940065, + 1940066, + 1940067, + 1940068, + 1940069, + 1940070, + 1940071, + 1940072, + 1940073, + 1940074, + 1940075, + 1940076, + 1940077, + 1940078, + 1940079, + 1940080, + 1940081, + 1940082, + 1940083, + 1940084, + 1940085, + 1940086, + 1940087, + 1940088, + 1940089, + 1940090, + 1940091, + 1940092, + 1940093, + 1940094, + 1940095, + 1940096, + 1940097, + 1940098, + 1940099, + 1940100, + 1940101, + 1940102, + 1940103, + 1940104, + 1940105, + 1940106, + 1940107, + 1940108, + 1940109, + 1940110, + 1940111, + 1940112, + 1940113, + 1940114, + 1940115, + 1940116, + 1940117, + 1940118, + 1940119, + 1940120, + 1940121, + 1940122, + 1940123, + 1940124, + 1940125, + 1940126, + 1940127, + 1940128, + 1940129, + 1940130, + 1940131, + 1940132, + 1940133, + 1940134, + 1940135, + 1940136, + 1940137, + 1940138, + 1940139, + 1940140, + 1940141, + 1940142, + 1940143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_194/data.jsonl b/retranslated_vi_pubmed/chunk_194/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..9a04fc4760b3181b0dbe64e5955f6f5b5a78f055 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_194/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1940000, "en": "The grafting of dimethyl amino ethyl methacrylate (DMAEMA) onto styrene-butadiene-styrene triblock copolymer (SBS) membrane was subsequently conducted by UV-radiation induced graft copolymerization without degassing to obtain the SBS-g-DMAEMA copolymer membrane. The substituted amino groups on the SBS-g-DMAEMA graft copolymer membrane were quaternized with iodomethane, and then the membrane was treated with heparin to prepare the heparin-containing SBS-g-DMAEMA copolymer membrane (SBS-g-DMAEMA-HEP). The graft copolymer membrane (SBS-g-DMAEMA) and the heparin-containing SBS-g-DMAEMA copolymer membrane (SBS-g-DMAEMA-HEP) were characterized by FTIR spectroscopy. The heparin content was determined by toluidine blue heparin assay. Contact angle, water content, and protein adsorption of fibrinogen and albumin experiments were also performed to evaluate the effect of graft amount and heparin content on the biocompatibility of SBS-g-DMAEMA and SBS-g-DMAEMA-HEP graft copolymer membranes. By using Kaelble's equation, the surface tension of SBS-g-DMAEMA and SBS-g-DMAEMA-HEP were determined. It was found that with increasing grafting amount and the heparin content, the surface tension and water content of SBS-g-DMAEMA membrane increased, whereas the contact angle decreased. The amount of the adsorption of albumin and fibrinogen decreased with increasing graft amount and heparin content. However, there was a minimum for adsorption of proteins in the SBS-g-DMAEMA and SBS-g-DMAEMA-HEP membranes.", "gemini": "Sau đó, quá trình ghép dimethyl amino ethyl methacrylate (DMAEMA) vào màng ba khối copolymer styrene-butadiene-styrene (SBS) được thực hiện bằng cách ghép copolymer cảm ứng bức xạ UV mà không cần khử khí để thu được màng copolymer SBS-g-DMAEMA. Các nhóm amino thay thế trên màng ghép copolymer SBS-g-DMAEMA được quatern hóa bằng iodomethane, sau đó màng được xử lý bằng heparin để điều chế màng copolymer SBS-g-DMAEMA chứa heparin (SBS-g-DMAEMA-HEP). Màng ghép copolymer (SBS-g-DMAEMA) và màng copolymer SBS-g-DMAEMA chứa heparin (SBS-g-DMAEMA-HEP) được đặc trưng bằng quang phổ FTIR. Hàm lượng heparin được xác định bằng xét nghiệm heparin toluidine blue. Góc tiếp xúc, hàm lượng nước và thí nghiệm hấp phụ protein của fibrinogen và albumin cũng được thực hiện để đánh giá tác động của lượng ghép và hàm lượng heparin lên khả năng tương thích sinh học của màng ghép copolymer SBS-g-DMAEMA và SBS-g-DMAEMA-HEP. Bằng cách sử dụng phương trình Kaelble, sức căng bề mặt của SBS-g-DMAEMA và SBS-g-DMAEMA-HEP được xác định. Người ta thấy rằng khi lượng ghép và hàm lượng heparin tăng, sức căng bề mặt và hàm lượng nước của màng SBS-g-DMAEMA tăng, trong khi góc tiếp xúc giảm. Lượng hấp phụ albumin và fibrinogen giảm khi lượng ghép và hàm lượng heparin tăng. Tuy nhiên, có một lượng tối thiểu hấp phụ protein trong màng SBS-g-DMAEMA và SBS-g-DMAEMA-HEP."} +{"idx": 1940001, "en": "An investigation was performed to study the mechanical performance of fiber-reinforced composite hip prostheses in a femur. The main objective of the study was to evaluate the effect of fiber orientation of a composite femoral implant on the response of the surrounding femoral bone. A three-dimensional finite element analysis was developed for analyzing a composite implant in the femur. A three-dimensional composite element was proposed to take into account ply drop-off due to a change of cross-section of the composite implant. The element could accommodate multidirectional layers and tapered composites. The material properties of the composite were treated as anisotropic and inhomogeneous while the properties of femoral bone were treated as anisotropic and homogenous. All the materials were assumed to behave linear-elastically. The thermoplastic graphite/PEEK material system was selected for the study. In this presentation, as the first part of the study, the development of the finite element analysis will be described. Numerical calculations were generated and compared with existing data and numerical results available from studies related to metal hip prostheses in the literature. Experiments on the composite hip implants were also conducted for further verification of the analysis and the computer simulations. In Part II, using the finite element code, an extensive study was performed to evaluate the stress/strain distributions, micromotions, and strain-energy density of the surrounding femoral bone, which have been related to initial fixation and long-term stability of the prosthesis in a femoral bone. Numerous fiber orientations were studied, and results of the calculations were compared with those generated by the prosthesis made of cobalt alloy and titanium alloy.", "gemini": "Một cuộc điều tra đã được thực hiện để nghiên cứu hiệu suất cơ học của chân tay giả hông tổng hợp gia cố bằng sợi trong xương đùi. Mục tiêu chính của nghiên cứu là đánh giá tác động của định hướng sợi của một vật cấy ghép xương đùi tổng hợp lên phản ứng của xương đùi xung quanh. Một phân tích phần tử hữu hạn ba chiều đã được phát triển để phân tích một vật cấy ghép tổng hợp trong xương đùi. Một phần tử tổng hợp ba chiều đã được đề xuất để tính đến sự giảm lớp do thay đổi mặt cắt ngang của vật cấy ghép tổng hợp. Phần tử này có thể chứa nhiều lớp đa hướng và vật liệu tổng hợp thon. Các tính chất vật liệu của vật liệu tổng hợp được coi là dị hướng và không đồng nhất trong khi các tính chất của xương đùi được coi là dị hướng và đồng nhất. Tất cả các vật liệu đều được coi là có tính đàn hồi tuyến tính. Hệ vật liệu than chì nhiệt dẻo/PEEK được chọn cho nghiên cứu. Trong bài thuyết trình này, là phần đầu tiên của nghiên cứu, quá trình phát triển phân tích phần tử hữu hạn sẽ được mô tả. Các phép tính số đã được tạo và so sánh với dữ liệu hiện có và kết quả số có sẵn từ các nghiên cứu liên quan đến chân tay giả hông kim loại trong tài liệu. Các thí nghiệm trên chân tay giả hông tổng hợp cũng được tiến hành để xác minh thêm về phân tích và mô phỏng máy tính. Trong Phần II, sử dụng mã phần tử hữu hạn, một nghiên cứu sâu rộng đã được thực hiện để đánh giá phân bố ứng suất/biến dạng, chuyển động vi mô và mật độ năng lượng biến dạng của xương đùi xung quanh, có liên quan đến cố định ban đầu và độ ổn định lâu dài của chân tay giả trong xương đùi. Nhiều định hướng sợi đã được nghiên cứu và kết quả tính toán đã được so sánh với các kết quả tạo ra từ chân tay giả làm bằng hợp kim coban và hợp kim titan."} +{"idx": 1940002, "en": "An investigation was performed to study the mechanical performance of fiber-reinforced composite hip prostheses. In Part I of the study, a three-dimensional finite element code was developed for analyzing a composite hip prosthesis in a femur. The material properties of the composite were treated as anisotropic and inhomogeneous while the properties of the femoral bone were treated as anisotropic and homogeneous. All the materials were assumed to behave linear-elastically. Thermoplastic graphite/PEEK material was selected for the study. No slippage was assumed at the interface between the implant and the surrounding femoral bone. In Part II, numerical simulations were performed using the code to study the performance of a composite prosthesis in the femur. The stress/strain distributions, micromotions, and strain energy density of the surrounding femoral bone were evaluated and found to be related to initial fixation and long-term stability of the prosthesis in the femur. Numerous fiber orientations were studied, and the results of the calculations were compared with those generated from a prosthesis made of cobalt chrome and Ti-6Al-4V titanium alloys. Based on the analysis, it was shown that compared to conventional metallic implants more favorable stresses and deformations could be generated in the femur using composite implants. In addition, by changing fiber orientations according to femoral loads, a composite implant could be designed specifically for the left or the right femur.", "gemini": "Một cuộc điều tra đã được thực hiện để nghiên cứu hiệu suất cơ học của chân tay giả hông tổng hợp gia cố bằng sợi. Trong Phần I của nghiên cứu, một mã phần tử hữu hạn ba chiều đã được phát triển để phân tích một chân tay giả hông tổng hợp trong xương đùi. Các tính chất vật liệu của vật liệu tổng hợp được coi là dị hướng và không đồng nhất trong khi các tính chất của xương đùi được coi là dị hướng và đồng nhất. Tất cả các vật liệu đều được coi là có tính đàn hồi tuyến tính. Vật liệu than chì nhiệt dẻo/PEEK được chọn cho nghiên cứu. Không có trượt nào được coi là xảy ra ở giao diện giữa vật cấy ghép và xương đùi xung quanh. Trong Phần II, các mô phỏng số đã được thực hiện bằng cách sử dụng mã để nghiên cứu hiệu suất của chân tay giả tổng hợp trong xương đùi. Phân bố ứng suất/biến dạng, chuyển động vi mô và mật độ năng lượng biến dạng của xương đùi xung quanh đã được đánh giá và thấy có liên quan đến cố định ban đầu và độ ổn định lâu dài của chân tay giả trong xương đùi. Nhiều định hướng sợi đã được nghiên cứu và kết quả tính toán đã được so sánh với các kết quả tạo ra từ một chân tay giả làm bằng hợp kim crom coban và hợp kim titan Ti-6Al-4V. Dựa trên phân tích, đã chỉ ra rằng so với các vật cấy ghép kim loại thông thường, có thể tạo ra ứng suất và biến dạng thuận lợi hơn trong xương đùi bằng cách sử dụng vật cấy ghép tổng hợp. Ngoài ra, bằng cách thay đổi định hướng sợi theo tải trọng của xương đùi, có thể thiết kế một vật cấy ghép tổng hợp đặc biệt cho xương đùi trái hoặc phải."} +{"idx": 1940003, "en": "A new, in vitro ultrasound-based method to measure the thickness of acetabular polyethylene components was developed and applied to 26 uncomplicated autopsy retrieved components and 40 unused components. The average age at total hip arthroplasty was 62 years and the average time in service of the retrieved components was 49 months. The clinical notes indicated that each of the arthroplasties was functioning well at the time of the patients death. Thickness distributions in the retrieved components had two distinct patterns. Eighteen of the retrieved components (69%) had their thinnest areas self-contained and located near the polar point. In the other 8 retrieved components (31%) the areas of minimum thickness were adjacent to the rim. Thickness distribution in the unused cups was predominately concentric with the thinnest area located near the polar point (85% of the cups). Detection limits for dimensional change were established based on the variability found in the unused liners. Fifteen of the 26 retrieved components (58%) had areas of reduced thickness which exceeded the detection limits; the average thickness reduction rate for these components was 0.076 mm per year. The other 11 retrieved components (42%) lacked such areas. The 15 cups with areas of reduced thickness had a longer time in service (63 +/- 18 months) than the 11 cups without areas of reduced thickness (32 +/- 25 months) (p = 0.003), but no other clinical factor (age, gender, Harris hip score, size and inclination of the cup, type of femoral fixation) was associated with these 15 cups. Cylindrical models to estimate volumetric change tended to underestimate the actual changes, suggesting that the actual particulate burden may be greater than previously appreciated. Finding that the pattern of thickness reduction can vary suggests that distinctive hip loading modes may be present postoperatively in patients with total hip arthroplasty. The wear rates of these components are consistent with wear rates calculated from radiographic data for well-functioning implants and are considerably lower than wear rates calculated for surgically-retrieved implants, indicating that autopsy-derived retrievals may be more representative of the majority of components currently in service than surgically-derived retrievals.", "gemini": "Một phương pháp siêu âm trong ống nghiệm mới để đo độ dày của các thành phần polyethylene ổ chảo đã được phát triển và áp dụng cho 26 thành phần lấy từ tử thi không biến chứng và 40 thành phần chưa sử dụng. Độ tuổi trung bình khi phẫu thuật thay khớp háng toàn phần là 62 tuổi và thời gian trung bình sử dụng các thành phần lấy từ tử thi là 49 tháng. Các ghi chú lâm sàng cho thấy rằng mỗi lần thay khớp háng đều hoạt động tốt tại thời điểm bệnh nhân tử vong. Phân bố độ dày ở các thành phần lấy từ tử thi có hai kiểu rõ rệt. Mười tám trong số các thành phần lấy từ tử thi (69%) có các vùng mỏng nhất của chúng được khép kín và nằm gần điểm cực. Ở 8 thành phần lấy từ tử thi còn lại (31%), các vùng có độ dày tối thiểu nằm cạnh vành. Phân bố độ dày ở các ổ chảo chưa sử dụng chủ yếu là đồng tâm với vùng mỏng nhất nằm gần điểm cực (85% các ổ chảo). Giới hạn phát hiện thay đổi về kích thước được thiết lập dựa trên sự thay đổi được tìm thấy ở các lớp lót chưa sử dụng. Mười lăm trong số 26 thành phần lấy từ tử thi (58%) có các vùng giảm độ dày vượt quá giới hạn phát hiện; tỷ lệ giảm độ dày trung bình đối với các thành phần này là 0,076 mm mỗi năm. 11 thành phần lấy từ tử thi còn lại (42%) không có các vùng như vậy. 15 ổ chảo có vùng giảm độ dày có thời gian sử dụng lâu hơn (63 +/- 18 tháng) so với 11 ổ chảo không có vùng giảm độ dày (32 +/- 25 tháng) (p = 0,003), nhưng không có yếu tố lâm sàng nào khác (tuổi, giới tính, Điểm háng Harris, kích thước và độ nghiêng của ổ chảo, loại cố định xương đùi) có liên quan đến 15 ổ chảo này. Các mô hình hình trụ để ước tính sự thay đổi thể tích có xu hướng đánh giá thấp các thay đổi thực tế, cho thấy rằng gánh nặng hạt thực tế có thể lớn hơn trước đây. Phát hiện rằng kiểu giảm độ dày có thể thay đổi cho thấy rằng các chế độ tải hông đặc biệt có thể có mặt sau phẫu thuật ở những bệnh nhân thay khớp háng toàn phần. Tỷ lệ mòn của các thành phần này phù hợp với tỷ lệ mòn được tính toán từ dữ liệu chụp X-quang đối với các implant hoạt động tốt và thấp hơn đáng kể so với tỷ lệ mòn được tính toán đối với các implant lấy ra bằng phẫu thuật, cho thấy rằng các mẫu lấy từ tử thi có thể đại diện nhiều hơn cho phần lớn các thành phần hiện đang sử dụng so với các mẫu lấy từ phẫu thuật."} +{"idx": 1940004, "en": "Resorption (defined as loss of ceramic coating because of cellular activity or dissolution) of ceramic coatings is a matter of concern for the long-term performance of ceramic-coated implants. A new fluorine-containing coating, fluorapatite (FA), has been shown to be more stable than hydroxyapatite (HA) in unloaded models. In a weight-bearing model in trabecular bone, we evaluated loss (defined as reduction of coating irrespective of type of mechanism) of HA and FA coatings during 25 weeks of implantation. Eight mature dogs had HA- or FA-coated implants inserted bilaterally into the weight-bearing region of the medial femoral condyle. Quantified loss of ceramic coating was estimated at the light microscopic level using stereological methods. The experiment showed significant loss of both types of coatings. However, no statistical difference in loss of ceramic coating was found regarding surface area implant coverage, volume, and thickness (p = 0.77, p = 0.13, p = 0.56, p = 0.23, respectively). Completely resorbed HA coating was replaced by 36 +/- 6.0% (range: 26-42) bone in direct contact with the implant surface compared with 29 +/- 16.0% (range: 12-59) for FA (p = 0.40), suggesting that the implant was firmly fixed despite loss of the ceramic coating. Transmission electron microscopy in combination with electron energy spectroscopy and electron spectroscopic imaging showed that osteclast-like cells, osteocytes, macrophage-like cells, and fibroblasts had phagocytosed calcium-containing fragments, indicating cell-mediated resorption of the ceramic coating.", "gemini": "Sự tiêu hủy (được định nghĩa là mất lớp phủ gốm do hoạt động của tế bào hoặc hòa tan) của lớp phủ gốm là vấn đề đáng quan tâm đối với hiệu suất lâu dài của các implant phủ gốm. Một lớp phủ mới có chứa flo, florapatit (FA), đã được chứng minh là ổn định hơn hydroxyapatit (HA) trong các mô hình không tải. Trong một mô hình chịu trọng lượng ở xương xốp, chúng tôi đã đánh giá sự mất mát (được định nghĩa là giảm lớp phủ bất kể loại cơ chế nào) của lớp phủ HA và FA trong 25 tuần cấy ghép. Tám con chó trưởng thành đã cấy ghép implant phủ HA hoặc FA vào hai bên vào vùng chịu trọng lượng của lồi cầu xương đùi trong. Sự mất lớp phủ gốm được định lượng ước tính ở cấp độ kính hiển vi ánh sáng bằng các phương pháp lập thể học. Thí nghiệm cho thấy sự mất mát đáng kể của cả hai loại lớp phủ. Tuy nhiên, không tìm thấy sự khác biệt thống kê nào về sự mất lớp phủ gốm liên quan đến diện tích bề mặt bao phủ implant, thể tích và độ dày (lần lượt là p = 0,77, p = 0,13, p = 0,56, p = 0,23). Lớp phủ HA bị tiêu hủy hoàn toàn được thay thế bằng 36 +/- 6,0% (phạm vi: 26-42) xương tiếp xúc trực tiếp với bề mặt implant so với 29 +/- 16,0% (phạm vi: 12-59) đối với FA (p = 0,40), cho thấy rằng implant được cố định chắc chắn mặc dù lớp phủ gốm bị mất. Kính hiển vi điện tử truyền qua kết hợp với phổ năng lượng điện tử và hình ảnh phổ điện tử cho thấy các tế bào giống tế bào hủy xương, tế bào xương, tế bào giống đại thực bào và nguyên bào sợi đã thực bào các mảnh chứa canxi, chỉ ra rằng sự tiêu hủy lớp phủ gốm do tế bào trung gian."} +{"idx": 1940005, "en": "From January 1985 to December 1990, 598 consecutive New Jersey low-contact stress (LCS) total knee arthroplasties (TKAs) were performed for gonarthrosis. Among these 598 operations, 322 were with rotating platform elements and 276 were with meniscal bearing elements. During 5 to 8 years of follow-up, eight knees had severe symptomatic polyethylene failure that required revision surgery; all eight cases were meniscal bearing types. The failed polyethylene inserts were retrieved and studied. It was observed that there were four probable failure mechanisms associated with the catastrophic polyethylene wear. First, insufficient thickness of the meniscal bearing was the major reason for wear. Second, the malpositioning of the metal tibial tray in the transverse plane resulted in the breaking of the meniscal bearing. Third, the inability of the patellar to rotate due to tissue ingrowth made the polyethylene break. Last, yellowing of the subsurface of the meniscal bearing was a sign of polyethylene failure. These four possible failure mechanisms are all associated with the design of the meniscal bearing type of LCS knee prostheses. Therefore, it is suggested that the design of the LCS knee prosthesis should be modified.", "gemini": "Từ tháng 1 năm 1985 đến tháng 12 năm 1990, 598 ca phẫu thuật thay khớp gối toàn phần (TKA) ứng suất tiếp xúc thấp New Jersey (LCS) liên tiếp đã được thực hiện để điều trị bệnh thoái hóa khớp gối. Trong số 598 ca phẫu thuật này, 322 ca có các thành phần nền tảng quay và 276 ca có các thành phần chịu men. Trong quá trình theo dõi từ 5 đến 8 năm, tám đầu gối bị hỏng polyethylene có triệu chứng nghiêm trọng cần phải phẫu thuật sửa lại; tất cả tám trường hợp đều là loại chịu men. Các miếng chèn polyethylene bị hỏng đã được lấy ra và nghiên cứu. Người ta quan sát thấy rằng có bốn cơ chế hỏng có thể xảy ra liên quan đến tình trạng mòn polyethylene thảm khốc. Đầu tiên, độ dày không đủ của ổ chịu men là lý do chính gây mòn. Thứ hai, việc định vị sai khay chày kim loại trên mặt phẳng ngang dẫn đến vỡ ổ chịu men. Thứ ba, sự bất lực của xương bánh chè do mô phát triển vào trong khiến polyethylene bị vỡ. Cuối cùng, sự ố vàng của bề mặt dưới của ổ chịu men là dấu hiệu của hỏng polyethylene. Cả bốn cơ chế hỏng có thể xảy ra này đều liên quan đến thiết kế loại ổ gối LCS chịu men. Do đó, người ta cho rằng nên sửa đổi thiết kế của gối nhân tạo LCS."} +{"idx": 1940006, "en": "Bioactive glass fibers were investigated for use as a fixation vehicle between a low modulus, polymeric composite and bone tissue. In an initial pilot study, bioactive glass fiber/polysulfone composites and all-polysulfone control rods were implanted into the rabbit tibia; the study was subsequently expanded with implantation into the rabbit femur. Bone tissue exhibited direct contact with the glass fibers and adjacent polymer matrix and displayed a mechanical bond between the composite and bone tissue after six weeks implantation. Interfacial bond strengths after six weeks implantation averaged 12.4 MPa, significantly higher than those of the all-polymer controls. Failure sites for the composite at six weeks generally occurred in the bone tissue or composite, whereas the failure site for the polymer implants occurred exclusively at the implant/tissue interface. The bioactive glass fiber/polysulfone composite achieved fixation to bone tissue through a triple mechanism: a bond to the bioactive glass fiber, mechanical interlocking between the tissue and glass fibers, and close apposition and possible chemical bond between the portions of the polymer and bone tissue. This last mechanism resulted from an overspill of bioactivity reactions from the fibers onto the surface of the surrounding polymer which we call the \"halo\" effect.", "gemini": "Các sợi thủy tinh hoạt tính sinh học đã được nghiên cứu để sử dụng như một phương tiện cố định giữa một composite polyme có mô đun thấp và mô xương. Trong một nghiên cứu thí điểm ban đầu, các composite sợi thủy tinh hoạt tính sinh học/polysulfone và các thanh điều khiển toàn polysulfone đã được cấy ghép vào xương chày của thỏ; nghiên cứu sau đó được mở rộng với việc cấy ghép vào xương đùi của thỏ. Mô xương thể hiện sự tiếp xúc trực tiếp với các sợi thủy tinh và ma trận polyme liền kề và thể hiện liên kết cơ học giữa composite và mô xương sau sáu tuần cấy ghép. Độ bền liên kết giao diện sau sáu tuần cấy ghép trung bình là 12,4 MPa, cao hơn đáng kể so với các đối chứng toàn polyme. Các vị trí hỏng của composite sau sáu tuần nói chung xảy ra ở mô xương hoặc composite, trong khi vị trí hỏng của các implant polyme xảy ra riêng biệt tại giao diện implant/mô. Composite sợi thủy tinh hoạt tính sinh học/polysulfone đạt được sự cố định với mô xương thông qua cơ chế ba: liên kết với sợi thủy tinh hoạt tính sinh học, liên kết cơ học giữa mô và sợi thủy tinh, và sự tiếp cận gần và liên kết hóa học có thể có giữa các phần của polyme và mô xương. Cơ chế cuối cùng này là kết quả của sự tràn phản ứng hoạt tính sinh học từ các sợi lên bề mặt của polyme xung quanh mà chúng ta gọi là hiệu ứng \"quầng sáng\"."} +{"idx": 1940007, "en": "This article is a personal reflection on a long career in cardiovascular nursing. The author focuses on some of the lessons learned and on individuals pivotal to her learning. Major lessons relate to managing stress by maintaining a sense of perspective in the face of minor frustrations, creating significance from seemingly insignificant interactions and situations, and making career decisions that are based on personal as well as monetary rewards. She also discusses the satisfaction of developing expertise in a health care specialty and the importance of finding clinical mentors. Finally, she reflects on the challenges and rewards of teaching.", "gemini": "Bài viết này là một sự phản ánh cá nhân về một sự nghiệp dài trong ngành điều dưỡng tim mạch. Tác giả tập trung vào một số bài học kinh nghiệm và những cá nhân đóng vai trò quan trọng trong quá trình học tập của cô. Những bài học chính liên quan đến việc quản lý căng thẳng bằng cách duy trì cảm giác nhìn nhận đúng bản chất trước những thất vọng nhỏ, tạo ra ý nghĩa từ những tương tác và tình huống dường như không đáng kể và đưa ra các quyết định về nghề nghiệp dựa trên cả phần thưởng cá nhân và tiền bạc. Cô cũng thảo luận về sự hài lòng khi phát triển chuyên môn trong một chuyên ngành chăm sóc sức khỏe và tầm quan trọng của việc tìm kiếm người cố vấn lâm sàng. Cuối cùng, cô ấy phản ánh về những thách thức và phần thưởng của việc giảng dạy."} +{"idx": 1940008, "en": "This article describes events that illustrate why Dr Kathleen Dracup is an extraordinary mentor to students and colleagues. Her commitment to the advancement of her students and colleagues, her gentle but persistent encouragement to grow, and her generosity in providing pivotal opportunities are highlighted. Examples of her important contributions with students to cardiovascular research and successes enjoyed by students as a result of their relationships with Dr Dracup are presented.", "gemini": "Bài viết này mô tả các sự kiện minh họa tại sao Tiến sĩ Kathleen Dracup là một người cố vấn phi thường đối với sinh viên và đồng nghiệp. Cam kết của cô ấy đối với sự tiến bộ của sinh viên và đồng nghiệp, sự động viên nhẹ nhàng nhưng bền bỉ để phát triển và sự hào phóng trong việc cung cấp các cơ hội quan trọng được nêu bật. Các ví dụ về những đóng góp quan trọng của cô với sinh viên vào nghiên cứu tim mạch và những thành công mà sinh viên đạt được nhờ mối quan hệ của họ với Tiến sĩ Dracup được trình bày."} +{"idx": 1940009, "en": "Throughout history, master weavers have described the weaving of a tapestry as a calling, as transformation, as healing, or as a sacred work. The early, single threads of my tapestry have taken on new meaning over time as I have become clearer in mind and spirit about my mission of bringing holism and healing to the forefront of nursing. This article reveals some of the threads, dominant patterns, and pearls that have been woven into my tapestry of holism.", "gemini": "Trong suốt chiều dài lịch sử, những người thợ dệt bậc thầy đã mô tả việc dệt thảm là một tiếng gọi, là sự chuyển đổi, là sự chữa lành hay là một công việc thiêng liêng. Những sợi chỉ đơn lẻ ban đầu của tấm thảm của tôi đã mang một ý nghĩa mới theo thời gian khi tôi trở nên sáng suốt hơn về mặt trí tuệ và tinh thần về sứ mệnh đưa tính toàn diện và sự chữa lành lên hàng đầu trong điều dưỡng. Bài viết này tiết lộ một số sợi chỉ, các mẫu chủ đạo và những viên ngọc đã được dệt vào tấm thảm toàn diện của tôi."} +{"idx": 1940010, "en": "The powerful relationship between a teacher and student of nursing is described through stories that illustrate three lessons: (1) Caring gets results, (2) family comes first, and (3) passionate commitment is contagious. These are the lessons I learned from my mentor, Dr Cathie Guzzetta. Textbooks can tell part of a story, but the \"dance for two\" between a special teacher and a student can change perspectives and lives forever.", "gemini": "Mối quan hệ mạnh mẽ giữa một giáo viên và sinh viên điều dưỡng được mô tả thông qua những câu chuyện minh họa cho ba bài học: (1) Sự quan tâm mang lại kết quả, (2) gia đình là trên hết và (3) sự cam kết đầy nhiệt huyết có sức lan tỏa. Đây là những bài học tôi học được từ người cố vấn của mình, Tiến sĩ Cathie Guzzetta. Sách giáo khoa có thể kể một phần của câu chuyện, nhưng \"điệu nhảy đôi\" giữa một người thầy đặc biệt và một học trò có thể thay đổi quan điểm và cuộc sống mãi mãi."} +{"idx": 1940011, "en": "Several themes emerged as I reviewed my career and development as a leader in cardiovascular nursing. An inherent part of my personality is to analyze situations, identify needed action, and figure out appropriate solutions. I recognized that gaining expert knowledge, wise mentors, and constructive critique were essential if I was to develop the skill to make meaningful contributions. My greatest role satisfaction comes from facilitating the intersection of nursing with other disciplines and coaching and mentoring others. Volunteering in the American Heart Association has provided me with many opportunities, an extensive network, and invaluable friendships.", "gemini": "Một số chủ đề nổi lên khi tôi xem xét lại sự nghiệp và sự phát triển của mình với tư cách là một nhà lãnh đạo trong điều dưỡng tim mạch. Một phần vốn có trong tính cách của tôi là phân tích các tình huống, xác định hành động cần thiết và tìm ra các giải pháp thích hợp. Tôi nhận ra rằng việc có được kiến thức chuyên môn, những người cố vấn khôn ngoan và những lời phê bình mang tính xây dựng là điều cần thiết nếu tôi muốn phát triển kỹ năng để có những đóng góp có ý nghĩa. Sự hài lòng lớn nhất trong vai trò của tôi đến từ việc tạo điều kiện cho sự giao thoa giữa điều dưỡng với các chuyên ngành khác cũng như huấn luyện và cố vấn cho những người khác. Việc làm tình nguyện tại Hiệp hội Tim mạch Hoa Kỳ đã mang đến cho tôi nhiều cơ hội, một mạng lưới rộng lớn và những tình bạn vô giá."} +{"idx": 1940012, "en": "Martha N. Hill, PhD, RN, FAAN, is a world-renowned researcher, educator, and nursing leader. Her election as president of the American Heart Association, effective June 1997, places her in one of the highest regarded positions in the field of cardiology. Despite her success on a national and international level, Dr. Hill has managed to continue to mentor and conduct clinical research with her nursing colleagues and students at The Johns Hopkins University in Baltimore, Maryland.", "gemini": "Martha N. Hill, Tiến sĩ, Điều dưỡng, FAAN, là nhà nghiên cứu, nhà giáo dục và là nhà lãnh đạo điều dưỡng nổi tiếng thế giới. Việc bà được bầu làm chủ tịch của Hiệp hội Tim mạch Hoa Kỳ có hiệu lực từ tháng 6 năm 1997 đã đưa bà lên một trong những vị trí được kính trọng nhất trong lĩnh vực tim mạch. Bất chấp thành công của mình ở cấp độ quốc gia và quốc tế, Tiến sĩ Hill đã tiếp tục hướng dẫn và tiến hành nghiên cứu lâm sàng với các đồng nghiệp và sinh viên điều dưỡng của bà tại Đại học Johns Hopkins ở Baltimore, Maryland."} +{"idx": 1940013, "en": "Cardiovascular nursing is a relatively young specialty that has attracted a growing number at clinicians, teachers, and researchers. This article describes the experiences of one nurse who has witnessed and helped to shape the changes in cardiovascular nursing that have occurred over a period of 25 years. She describes her career as a cardiovascular nurse and as a mentor for others entering the field. She recalls some of the many lessons learned from her work in clinical and academic settings and shares with the readers of The Journal of Cardiovascular Nursing how these lessons shaped her work as a teacher and mentor.", "gemini": "Điều dưỡng tim mạch là một chuyên khoa tương đối trẻ đã thu hút một số lượng lớn các bác sĩ lâm sàng, giáo viên và nhà nghiên cứu. Bài viết này mô tả những kinh nghiệm của một y tá đã chứng kiến và giúp định hình những thay đổi trong điều dưỡng tim mạch đã diễn ra trong khoảng thời gian 25 năm. Cô ấy mô tả sự nghiệp của mình với tư cách là một y tá tim mạch và là người cố vấn cho những người khác bước vào lĩnh vực này. Cô ấy nhớ lại một số bài học kinh nghiệm rút ra được từ công việc của mình trong các môi trường lâm sàng và học thuật và chia sẻ với độc giả của Tạp chí Điều dưỡng Tim mạch về cách những bài học này định hình công việc của cô ấy với tư cách là một giáo viên và người cố vấn."} +{"idx": 1940014, "en": "This tribute to Dr Marguerite Kinney, a transformational leader in cardiovascular nursing, highlights her influence on the lives and careers of previous students and those she has mentored. Examples of Dr Kinney's excellence in teaching, leadership, scholarship and research, and creative thinking within the discipline of nursing are provided.", "gemini": "Lời tri ân này dành cho Tiến sĩ Marguerite Kinney, một nhà lãnh đạo có sức ảnh hưởng trong lĩnh vực điều dưỡng tim mạch, nêu bật tầm ảnh hưởng của bà đối với cuộc sống và sự nghiệp của những học sinh trước đây và những người mà bà đã hướng dẫn. Các ví dụ về sự xuất sắc của Tiến sĩ Kinney trong giảng dạy, lãnh đạo, học bổng và nghiên cứu, và tư duy sáng tạo trong ngành điều dưỡng được cung cấp."} +{"idx": 1940015, "en": "Family members, teachers, and colleagues are all influential in our socialization as professionals and nurse scientists. This article discusses lessons learned from mentors and facilitators during the various stages in my career, including clinical practice, graduate education, and academia. The value of postdoctoral experiences, whether formal or informal, in the pursuit of excellence is also discussed. Finally, the need to maintain a balanced quality of life is stressed in all phases of a career trajectory to facilitate attainment of our goals and the ability to maintain a standard of excellence.", "gemini": "Các thành viên gia đình, giáo viên và đồng nghiệp đều có ảnh hưởng đến quá trình xã hội hóa của chúng ta với tư cách là các chuyên gia và nhà khoa học điều dưỡng. Bài viết này thảo luận về những bài học kinh nghiệm từ những người cố vấn và những người tạo điều kiện trong các giai đoạn khác nhau trong sự nghiệp của tôi, bao gồm thực hành lâm sàng, giáo dục sau đại học và học viện. Giá trị của những kinh nghiệm sau tiến sĩ, dù chính thức hay không chính thức, trong việc theo đuổi sự xuất sắc cũng được thảo luận. Cuối cùng, nhu cầu duy trì chất lượng cuộc sống cân bằng được nhấn mạnh trong mọi giai đoạn của lộ trình sự nghiệp để tạo điều kiện đạt được các mục tiêu của chúng ta và khả năng duy trì tiêu chuẩn xuất sắc."} +{"idx": 1940016, "en": "This article presents the lessons learned from Dr Carolyn Murdaugh, a transformational leader in the field of cardiovascular nursing as well as the larger community of scientists and scholars. Dr Murdaugh has inspired us and empowered us with the love of learning, science, and caring for others and ourselves. We present aspects of Dr Murdaugh's leadership, including her abilities to create a vision, model professional commitment, the value of hard work, and the creation of balance.", "gemini": "Bài viết này trình bày những bài học kinh nghiệm từ Tiến sĩ Carolyn Murdaugh, một nhà lãnh đạo chuyển đổi trong lĩnh vực điều dưỡng tim mạch cũng như cộng đồng các nhà khoa học và học giả lớn hơn. Tiến sĩ Murdaugh đã truyền cảm hứng cho chúng tôi và trao quyền cho chúng tôi bằng tình yêu học hỏi, khoa học và sự quan tâm đến người khác và chính chúng tôi. Chúng tôi trình bày các khía cạnh về khả năng lãnh đạo của Tiến sĩ Murdaugh, bao gồm khả năng tạo ra tầm nhìn, mô hình cam kết chuyên nghiệp, giá trị của sự chăm chỉ và việc tạo ra sự cân bằng."} +{"idx": 1940017, "en": "Dr Quaal presents an account of her career as a cardiovascular clinical specialist from an early inspiration role model to the mentors who had an impact on her professional development. Insights and inspirations that form the conceptual framework for this article include preparatory pearls of wisdom, the stimulus of adversity, experiencing fulfillment, salient lessons learned, and viewing the journey of life with new eyes. Insights and inspirations originated from mentors ranging from a small Midwestern town upbringing to Baragwanath Hospital in South Africa. Dr Quaal reflects on nearly 25 years as a cardiovascular specialist and pays tribute to all who mentored her with their personal and professional wisdom.", "gemini": "Tiến sĩ Quaal trình bày một bản tường trình về sự nghiệp của bà với tư cách là một chuyên gia lâm sàng về tim mạch từ một hình mẫu truyền cảm hứng ban đầu đến những người cố vấn có ảnh hưởng đến sự phát triển chuyên môn của bà. Những hiểu biết sâu sắc và nguồn cảm hứng tạo nên khuôn khổ khái niệm cho bài viết này bao gồm những viên ngọc quý chuẩn bị cho sự khôn ngoan, sự kích thích của nghịch cảnh, trải nghiệm sự viên mãn, những bài học nổi bật đã học được và nhìn hành trình cuộc sống bằng con mắt mới. Những hiểu biết sâu sắc và nguồn cảm hứng bắt nguồn từ những người cố vấn, từ một thị trấn nhỏ ở miền Trung Tây đến Bệnh viện Baragwanath ở Nam Phi. Tiến sĩ Quaal suy ngẫm về gần 25 năm làm chuyên gia tim mạch và bày tỏ lòng biết ơn đối với tất cả những người đã hướng dẫn bà bằng sự khôn ngoan cá nhân và chuyên môn của họ."} +{"idx": 1940018, "en": "To be a transformational leader in nursing, one must have forever changed the course of our practice. This article highlights the qualities of a great leader, Susan J. Quaal, PhD, APRN, CVS, CCRN. Described are examples of Susan's incredible clinical expertise and also the attributes that make her such a dynamic leader in all domains of the clinical nurse specialist role: Practitioner, educator/mentor, consultant, leader/administrator, and researcher. Interwoven in this article, you will also find the threads of humility and charity that make Susan such an extraordinary human being and a blessing to all the lives she touches.", "gemini": "Để trở thành một nhà lãnh đạo chuyển đổi trong điều dưỡng, người ta phải mãi mãi thay đổi quá trình hành nghề của chúng ta. Bài viết này nêu bật những phẩm chất của một nhà lãnh đạo vĩ đại, Susan J. Quaal, Tiến sĩ, APRN, CVS, CCRN. Được mô tả là những ví dụ về chuyên môn lâm sàng đáng kinh ngạc của Susan và cả những thuộc tính khiến cô trở thành một nhà lãnh đạo năng động trong mọi lĩnh vực của vai trò chuyên gia điều dưỡng lâm sàng: Người hành nghề, nhà giáo dục/người cố vấn, nhà tư vấn, nhà lãnh đạo/quản trị viên và nhà nghiên cứu. Đan xen trong bài viết này, bạn cũng sẽ tìm thấy những sợi chỉ của sự khiêm tốn và lòng bác ái khiến Susan trở thành một con người phi thường và là một phước lành cho tất cả những cuộc đời mà cô ấy chạm đến."} +{"idx": 1940019, "en": "This paper could also be titled, \"Fortunately, I Was Not Electrocuted.\" Dr Winslow candidly discusses her bathing and toileting research as well as her more recent research. Because she believes that failure is a good teacher, she describes her blunders as well as her accomplishments. In addition, she provides criteria helpful in selecting a good clinical problem for study and stresses the importance of identifying and investigating nursing rituals. Approaches that enhance the likelihood of success in research, public speaking, publishing, and in life are also shared.", "gemini": "Bài báo này cũng có thể được đặt tiêu đề là \"May mắn thay, tôi đã không bị điện giật\". Tiến sĩ Winslow thẳng thắn thảo luận về nghiên cứu tắm rửa và vệ sinh của bà cũng như nghiên cứu gần đây hơn của bà. Bởi vì cô ấy tin rằng thất bại là một giáo viên giỏi, cô ấy mô tả những sai lầm cũng như những thành tựu của mình. Ngoài ra, cô ấy cung cấp các tiêu chí hữu ích trong việc lựa chọn một vấn đề lâm sàng tốt để nghiên cứu và nhấn mạnh tầm quan trọng của việc xác định và điều tra các nghi thức điều dưỡng. Những cách tiếp cận làm tăng khả năng thành công trong nghiên cứu, diễn thuyết trước công chúng, xuất bản và trong cuộc sống cũng được chia sẻ."} +{"idx": 1940020, "en": "This article describes the development of a mentoring relationship between the author and Elizabeth Winslow since it began in 1990. It includes numerous examples of ways in which the mentor, Dr Winslow, provided inspiration, support, and encouragement during high and low points of the author's professional development. The process by which Dr Winslow became the author's mentor and the nature of the current mentor/mentee relationship challenge commonly held beliefs about how mentoring relationships begin and unfold.", "gemini": "Bài viết này mô tả sự phát triển của mối quan hệ cố vấn giữa tác giả và Elizabeth Winslow kể từ khi nó bắt đầu vào năm 1990. Nó bao gồm nhiều ví dụ về những cách mà người cố vấn, Tiến sĩ Winslow, đã cung cấp cảm hứng, hỗ trợ và động viên trong những thời điểm thăng trầm của sự phát triển nghề nghiệp của tác giả. Quá trình mà Tiến sĩ Winslow trở thành cố vấn của tác giả và bản chất của mối quan hệ cố vấn/người được cố vấn hiện tại thách thức những niềm tin thường thấy về cách các mối quan hệ cố vấn bắt đầu và diễn ra."} +{"idx": 1940021, "en": "Current frontline antiepileptic drugs tend to fall into several cellular mechanistic categories, and these categories often correlate with the clinical spectrum of action of the various antiepileptic drugs. Many antiepileptic drugs effective in control of partial and generalized tonic-clonic seizures are use- and voltage-dependent blockers of sodium channels. This mechanism selectively dampens pathologic activation of sodium channels, without interacting with normal sodium channel function. Examples include phenytoin, carbamazepine, valproic acid, and lamotrigine. Many antiepileptic drugs effective in control of generalized absence seizures block low threshold calcium currents. Low threshold calcium channels are present in high densities in thalamic neurons, and these channels trigger regenerative bursts that drive normal and pathologic thalamocortical rhythms, including the spike wave discharges of absence seizures. Examples include ethosuximide, trimethadione, and methsuximide. Several antiepileptic drugs that have varying clinical actions interact with the gamma-amino-butyric acid (GABA)ergic system. Diazepam and clonazepam selectively augment function of a subset of GABAA receptors, and these drugs are broad-spectrum antiepileptic drugs. In contrast, barbiturates augment function of all types of GABAA receptors, and are ineffective in control of generalized absence seizures, but effective in control of many other seizure types. Tiagabine and vigabatrin enhance cerebrospinal levels of GABA by interfering with reuptake and degradation of GABA, respectively. These antiepileptic drugs are effective in partial seizures. Lamotrigine is effective against both partial and generalized seizures, including generalized absence seizures. Its sole documented cellular mechanism of action is sodium channel block, a mechanism shared by phenytoin and carbamazepine. These drugs are ineffective against absence seizures. Consequently, unless there are unique aspects to the sodium channel block by lamotrigine, it seems unlikely that this mechanism alone could explain its broad clinical efficacy. Therefore, lamotrigine may have as yet uncharacterized cellular actions, which could combine with its sodium channel blocking actions, to account for its broad clinical efficacy.", "gemini": "Các thuốc chống động kinh tiền tuyến hiện nay có xu hướng rơi vào một số loại cơ chế tế bào và các loại này thường tương quan với phổ tác dụng lâm sàng của các thuốc chống động kinh khác nhau. Nhiều loại thuốc chống động kinh có hiệu quả trong việc kiểm soát các cơn động kinh cục bộ và cơn động kinh toàn thể có cơn co cứng - mất trương lực là thuốc chẹn kênh natri phụ thuộc vào điện áp và sử dụng. Cơ chế này làm giảm chọn lọc hoạt hóa bệnh lý của các kênh natri mà không tương tác với chức năng bình thường của kênh natri. Ví dụ bao gồm phenytoin, carbamazepine, axit valproic và lamotrigine. Nhiều loại thuốc chống động kinh có hiệu quả trong việc kiểm soát các cơn động kinh vắng điển hình sẽ chặn dòng canxi ngưỡng thấp. Các kênh canxi ngưỡng thấp có mật độ cao trong các tế bào thần kinh ở đồi thị và các kênh này kích hoạt các xung tái tạo thúc đẩy nhịp đồi - vỏ não bình thường và bệnh lý, bao gồm cả các xung sóng gai của các cơn động kinh vắng. Ví dụ bao gồm ethosuximide, trimethadione và methsuximide. Một số loại thuốc chống động kinh có tác dụng lâm sàng khác nhau tương tác với hệ thống axit gamma-aminobutyric (GABA). Diazepam và clonazepam làm tăng chọn lọc chức năng của một nhóm các thụ thể GABAA và các loại thuốc này là thuốc chống động kinh phổ rộng. Ngược lại, barbiturat làm tăng chức năng của tất cả các loại thụ thể GABAA và không có hiệu quả trong việc kiểm soát các cơn động kinh vắng điển hình, nhưng có hiệu quả trong việc kiểm soát nhiều loại cơn động kinh khác. Tiagabine và vigabatrin làm tăng nồng độ GABA trong dịch não tủy bằng cách can thiệp vào quá trình tái hấp thu và phân hủy GABA. Các loại thuốc chống động kinh này có hiệu quả trong các cơn động kinh cục bộ. Lamotrigine có hiệu quả chống lại cả các cơn động kinh cục bộ và toàn thể, bao gồm cả các cơn động kinh vắng điển hình. Cơ chế hoạt động tế bào duy nhất được ghi nhận của nó là chẹn kênh natri, một cơ chế được phenytoin và carbamazepine dùng chung. Các loại thuốc này không có hiệu quả đối với các cơn động kinh vắng. Do đó, trừ khi có những khía cạnh độc đáo đối với thuốc chẹn kênh natri của lamotrigine, có vẻ như không có khả năng cơ chế này có thể giải thích được hiệu quả lâm sàng rộng rãi của nó. Do đó, lamotrigine có thể có những tác dụng tế bào chưa được mô tả, có thể kết hợp với các tác dụng chẹn kênh natri của nó để giải thích cho hiệu quả lâm sàng rộng rãi của nó."} +{"idx": 1940022, "en": "The pharmacokinetics of lamotrigine have been studied in single and multiple dose studies in animals, normal volunteers, and patients with epilepsy. Lamotrigine exhibits first-order linear pharmacokinetics. Lamotrigine is well absorbed with bioavailability approaching 100%. The absorption is unaffected by food and there is no first-pass metabolism. The volume of distribution is between 1.25 and 1.47 L/kg and protein binding is about 55%. The half-life of lamotrigine is between 24.1 and 35 hours in drug naive adults but may be altered by enzyme inducing and inhibiting drugs. Clinical trials demonstrated no evidence of autoinduction or saturable metabolism. Younger children (0.17 to 5 years) eliminate lamotrigine faster than older children (5 to 10 years). Children may be more prone to enzyme induction than adults.", "gemini": "Dược động học của lamotrigine đã được nghiên cứu trong các nghiên cứu liều đơn và nhiều liều ở động vật, tình nguyện viên khỏe mạnh và bệnh nhân động kinh. Lamotrigine biểu hiện dược động học tuyến tính bậc nhất. Lamotrigine được hấp thu tốt với sinh khả dụng đạt tới 100%. Quá trình hấp thu không bị ảnh hưởng bởi thức ăn và không có quá trình chuyển hóa lần đầu. Thể tích phân bố từ 1,25 đến 1,47 L/kg và liên kết protein khoảng 55%. Thời gian bán hủy của lamotrigine từ 24,1 đến 35 giờ ở những người lớn chưa dùng thuốc nhưng có thể bị thay đổi bởi các loại thuốc gây cảm ứng và ức chế enzym. Các thử nghiệm lâm sàng không chứng minh được bằng chứng về tự cảm ứng hoặc chuyển hóa bão hòa. Trẻ em nhỏ hơn (0,17 đến 5 tuổi) đào thải lamotrigine nhanh hơn trẻ lớn hơn (5 đến 10 tuổi). Trẻ em có thể dễ bị cảm ứng enzym hơn người lớn."} +{"idx": 1940023, "en": "Lamotrigine is a phenyltriazine derivative with anticonvulsant properties that initially was tested in adults with partial seizures. Pharmacokinetics in adults includes a volume of distribution of 1.0 to 1.3 L/kg. Plasma protein binding is 55% and none of the metabolites are active as anticonvulsants. The placebo-controlled studies evaluated the responder rate as the percent of patients with 50% or greater seizure reduction when compared to baseline assessment. The responder rate for 300 mg per day was 20% and 36% for 500 mg per day dosages. Adverse events were infrequent but the occurrence of rash appeared to correlate with rapid ascension dosing and comedication with valproic acid. Slow titration is required when initiating treatment with lamotrigine. Accumulating experience in adults suggests that lamotrigine has a broad spectrum of effect; data from further controlled trials should suggest other clinically important forms of epilepsy that respond to lamotrigine.", "gemini": "Lamotrigine là dẫn xuất phenyltriazine có đặc tính chống co giật, ban đầu được thử nghiệm ở người lớn mắc chứng động kinh cục bộ. Dược động học ở người lớn bao gồm thể tích phân bố từ 1,0 đến 1,3 L/kg. Liên kết protein huyết tương là 55% và không có chất chuyển hóa nào hoạt động như thuốc chống co giật. Các nghiên cứu có đối chứng giả dược đã đánh giá tỷ lệ đáp ứng là phần trăm bệnh nhân giảm cơn động kinh 50% trở lên khi so sánh với đánh giá ban đầu. Tỷ lệ đáp ứng cho liều 300 mg mỗi ngày là 20% và 36% cho liều 500 mg mỗi ngày. Các tác dụng phụ không thường xuyên nhưng tình trạng phát ban có vẻ tương quan với việc tăng liều nhanh và dùng chung với axit valproic. Cần tăng liều chậm khi bắt đầu điều trị bằng lamotrigine. Kinh nghiệm tích lũy ở người lớn cho thấy lamotrigine có phổ tác dụng rộng; dữ liệu từ các thử nghiệm có đối chứng khác nên gợi ý các dạng động kinh quan trọng về mặt lâm sàng khác đáp ứng với lamotrigine."} +{"idx": 1940024, "en": "Individual seizures are described as either partial (localization related) or generalized depending on whether a localized area of cerebral cortex is symptomatically involved sufficient to impart to the seizure a focal signature or whether sufficient cortex is involved sufficiently rapidly to appear as if generalized from the outset. This distinction is of some diagnostic and therapeutic importance. However, it has become evident that the prognosis of the patient's epilepsy is less a function of the seizure type than it is of the etiology and of the nature of the syndrome that the seizures represent. This approach is stressed in this chapter as is the regret that there is frequently a delay in the acknowledgment of the usefulness of a new drug in childhood epilepsies, as exemplified by lamotrigine.", "gemini": "Các cơn động kinh riêng lẻ được mô tả là cục bộ (liên quan đến vị trí) hoặc toàn thể tùy thuộc vào việc một vùng cục bộ của vỏ não có liên quan đến triệu chứng đủ để truyền cho cơn động kinh một dấu hiệu cục bộ hay đủ vỏ não liên quan đủ nhanh để xuất hiện như toàn thể ngay từ đầu. Sự khác biệt này có một số tầm quan trọng về chẩn đoán và điều trị. Tuy nhiên, đã trở nên rõ ràng rằng tiên lượng động kinh của bệnh nhân ít phụ thuộc vào loại cơn động kinh hơn là nguyên nhân và bản chất của hội chứng mà các cơn động kinh đại diện. Cách tiếp cận này được nhấn mạnh trong chương này cũng như sự hối tiếc rằng thường có sự chậm trễ trong việc thừa nhận tính hữu ích của một loại thuốc mới trong bệnh động kinh ở trẻ em, như trường hợp lamotrigine."} +{"idx": 1940025, "en": "Lennox-Gastaut syndrome a combination of various generalized seizures including atypical absences and tonic seizures with generalized slow spike waves and mental deterioration, is often difficult to distinguish from a subgroup of myoclonic-astatic epilepsy, other generalized epilepsy syndromes, and various symptomatic generalized epilepsies. Conventional antiepileptic medication is poorly effective in this condition, particularly because various types of seizures respond differently to each given drug. Lamotrigine is effective in the various types of generalized seizures and efficacy has been demonstrated in Lennox-Gastaut syndrome. Given the potential of major mental deterioration within a matter of months in this condition, and the need of slow dose escalation in order to prevent skin rash, lamotrigine should be administered as soon as the diagnosis of Lennox-Gastaut syndrome is suspected. In addition, there is growing evidence that lamotrigine is also most useful in the subgroup of myoclonic-astatic epilepsy beginning in childhood, and that these patients should benefit from the drug like those affected by Lennox-Gastaut syndrome, as soon as the diagnosis is suspected. However, this drug may worsen other cases of myoclonic-astatic epilepsy beginning in infancy. These clinical observations add to the evidence for the need of clear diagnostic work-up before appropriate drug therapy is decided in pediatric epilepsy.", "gemini": "Hội chứng Lennox-Gastaut là sự kết hợp của các cơn động kinh toàn thể khác nhau bao gồm các cơn vắng mặt không điển hình và các cơn co cứng với sóng gai chậm toàn thể và suy giảm trí tuệ, thường khó phân biệt với một nhóm nhỏ động kinh cơ giật-vắng ý thức, các hội chứng động kinh toàn thể khác và các cơn động kinh toàn thể triệu chứng khác nhau. Thuốc chống động kinh thông thường kém hiệu quả trong tình trạng này, đặc biệt là vì các loại cơn động kinh khác nhau phản ứng khác nhau với từng loại thuốc nhất định. Lamotrigine có hiệu quả đối với các loại cơn động kinh toàn thể khác nhau và hiệu quả đã được chứng minh trong hội chứng Lennox-Gastaut. Với khả năng suy giảm trí tuệ nghiêm trọng trong vòng vài tháng trong tình trạng này và nhu cầu tăng liều chậm để ngăn ngừa phát ban da, lamotrigine nên được dùng ngay khi nghi ngờ chẩn đoán hội chứng Lennox-Gastaut. Ngoài ra, ngày càng có nhiều bằng chứng cho thấy lamotrigine cũng hữu ích nhất trong nhóm nhỏ động kinh cơ giật-vắng ý thức bắt đầu ở trẻ em và những bệnh nhân này sẽ được hưởng lợi từ thuốc giống như những bệnh nhân mắc hội chứng Lennox-Gastaut, ngay khi nghi ngờ chẩn đoán. Tuy nhiên, loại thuốc này có thể làm trầm trọng thêm các trường hợp động kinh cơ giật-vắng ý thức khác bắt đầu từ thời thơ ấu. Những quan sát lâm sàng này bổ sung thêm bằng chứng về sự cần thiết của việc chẩn đoán rõ ràng trước khi quyết định dùng thuốc thích hợp trong bệnh động kinh ở trẻ em."} +{"idx": 1940026, "en": "Epilepsy occurs in more than 30% of persons with mental retardation and up to 50% of those with multiple handicaps living in institutional settings. Treatment of epilepsy in this population presents many unique challenges. Through careful adherence to basic principles of diagnosis, classification, and antiepileptic drug use, as many as 80% of individuals can be successfully managed with one- or two-drug therapy and enjoy positive outcomes in the areas of community integration and increased independence. Rational polypharmacy with the newer anticonvulsant drugs offers additional opportunity for improving outcomes for these individuals, especially with the use of felbamate and lamotrigine, where broad-spectrum efficacy and positive impact on psychosocial functioning is being demonstrated. Further systematic study of new drugs in this population is clearly warranted.", "gemini": "Động kinh xảy ra ở hơn 30% những người bị chậm phát triển trí tuệ và lên đến 50% những người bị nhiều khuyết tật sống trong các cơ sở nuôi dưỡng. Điều trị động kinh ở nhóm đối tượng này đặt ra nhiều thách thức riêng biệt. Thông qua việc tuân thủ chặt chẽ các nguyên tắc cơ bản về chẩn đoán, phân loại và sử dụng thuốc chống động kinh, có tới 80% cá nhân có thể được điều trị thành công bằng liệu pháp một hoặc hai loại thuốc và đạt được kết quả tích cực trong các lĩnh vực hòa nhập cộng đồng và tăng tính độc lập. Dược phẩm đa hợp lý với các loại thuốc chống co giật mới hơn mang lại cơ hội bổ sung để cải thiện kết quả cho những cá nhân này, đặc biệt là với việc sử dụng felbamate và lamotrigine, nơi hiệu quả phổ rộng và tác động tích cực đến chức năng tâm lý xã hội đang được chứng minh. Nghiên cứu có hệ thống hơn nữa về các loại thuốc mới trong nhóm đối tượng này rõ ràng là cần thiết."} +{"idx": 1940027, "en": "Lamotrigine is a new antiepileptic drug that may possess unique cognitive and behavioral characteristics. Although lamotrigine can produce neurobehavioral toxicity, it is generally well tolerated. In one study directly comparing lamotrigine to placebo as add-on therapy in patients with intractable epilepsy, no objective cognitive effects were observed in a limited neuropsychological battery. Several studies have demonstrated favorable effects of lamotrigine on psychological well-being that were not explained by simple effects on seizure frequency and severity. In direct comparisons with carbamazepine and phenytoin, lamotrigine has been reported to produce positive effects on quality of life scales of patient perception. In addition, positive behavioral effects have also been observed in two blinded studies and several open trials for patients with severe mental disability and refractory epilepsy. Future studies with more extensive neuropsychological assessments are needed to delineate the differential cognitive and behavioral effects of lamotrigine in epilepsy and psychiatric disorders.", "gemini": "Lamotrigine là một thuốc chống động kinh mới có thể sở hữu các đặc điểm nhận thức và hành vi độc đáo. Mặc dù lamotrigine có thể gây độc thần kinh, nhưng nhìn chung vẫn được dung nạp tốt. Trong một nghiên cứu so sánh trực tiếp lamotrigine với giả dược như một liệu pháp bổ sung ở những bệnh nhân mắc chứng động kinh dai dẳng, không có tác dụng nhận thức khách quan nào được quan sát thấy trong một loạt các xét nghiệm tâm lý thần kinh hạn chế. Một số nghiên cứu đã chứng minh tác dụng có lợi của lamotrigine đối với sức khỏe tâm lý mà không được giải thích bằng các tác dụng đơn giản đối với tần suất và mức độ nghiêm trọng của cơn động kinh. Trong các so sánh trực tiếp với carbamazepine và phenytoin, lamotrigine đã được báo cáo là có tác dụng tích cực đối với thang đánh giá chất lượng cuộc sống của bệnh nhân. Ngoài ra, các tác dụng tích cực về hành vi cũng đã được quan sát thấy trong hai nghiên cứu mù đôi và một số thử nghiệm mở cho những bệnh nhân mắc chứng khuyết tật tâm thần nghiêm trọng và động kinh kháng thuốc. Các nghiên cứu trong tương lai với các đánh giá tâm lý thần kinh sâu rộng hơn là cần thiết để mô tả các tác dụng nhận thức và hành vi khác biệt của lamotrigine trong động kinh và các rối loạn tâm thần."} +{"idx": 1940028, "en": "Lamotrigine, like all antiepileptic drugs, can be effective when used as monotherapy or adjunctive therapy. In general, adverse effects are reduced when monotherapy is employed. The most frequent adverse effect prompting withdrawal of lamotrigine is rash. This potentially life-threatening adverse effect occurs more frequently in children, is increased when a rapid dose titration schedule is employed, and is greater when lamotrigine, is prescribed in combination with valproate. The availability of lamotrigine and other antiepileptic drugs represents a major advance for the treatment of childhood epilepsy. The challenge in using all of the new antiepileptic drugs, including lamotrigine, is to balance the expected improved efficacy with the potentially serious adverse effects.", "gemini": "Lamotrigine, giống như tất cả các loại thuốc chống động kinh, có thể có hiệu quả khi được sử dụng như một liệu pháp đơn trị hoặc liệu pháp bổ trợ. Nhìn chung, các tác dụng phụ sẽ giảm khi sử dụng liệu pháp đơn trị. Tác dụng phụ thường gặp nhất khiến phải ngừng dùng lamotrigine là phát ban. Tác dụng phụ có khả năng đe dọa tính mạng này xảy ra thường xuyên hơn ở trẻ em, tăng lên khi sử dụng lịch chuẩn độ liều nhanh và lớn hơn khi lamotrigine được kê đơn kết hợp với valproate. Việc có sẵn lamotrigine và các loại thuốc chống động kinh khác là một tiến bộ lớn trong việc điều trị động kinh ở trẻ em. Thách thức khi sử dụng tất cả các loại thuốc chống động kinh mới, bao gồm cả lamotrigine, là cân bằng hiệu quả cải thiện dự kiến với các tác dụng phụ nghiêm trọng có thể xảy ra."} +{"idx": 1940029, "en": "Autosomal dominant North Carolina macular dystrophy (NCMD) or central areolar pigment epithelial dystrophy (CAPED) is an allelic disorder that maps to an approximately 7.2 cM interval between DNA markers at D6S424 and D6S1671 on 6q14-q16.2. The further refinement of the disease locus has been hindered by the lack of additional recombination events involving the critical region. In this study, we have identified three multigeneration families of German descent who express the NCMD phenotype. Genotyping was carried out with a series of markers spanning approximately 53 cM around the NCMD locus, MCDR1. Genetic linkage between the markers and the disease phenotype in each of the families could be shown. Disease associated haplotypes were constructed and provide evidence for an ancestral founder for the German NCMD families. This haplotype analysis suggests that a 4.0 cM interval flanked by markers at D6S249 and D6S475 harbours the gene causing NCMD, facilitating further positional cloning approaches.", "gemini": "Bệnh thoái hóa hoàng điểm Bắc Carolina trội trên nhiễm sắc thể thường (NCMD) hoặc bệnh thoái hóa biểu mô sắc tố quanh núm trung tâm (CAPED) là một rối loạn alen được lập bản đồ trong khoảng 7,2 cM giữa các dấu hiệu DNA ở D6S424 và D6S1671 trên 6q14-q16.2. Việc tinh chỉnh hơn nữa vị trí bệnh đã bị cản trở do thiếu các sự kiện tái tổ hợp bổ sung liên quan đến vùng quan trọng. Trong nghiên cứu này, chúng tôi đã xác định được ba gia đình nhiều thế hệ gốc Đức biểu hiện kiểu hình NCMD. Kiểu gen đã được thực hiện với một loạt các dấu hiệu trải dài khoảng 53 cM xung quanh vị trí NCMD, MCDR1. Liên kết di truyền giữa các dấu hiệu và kiểu hình bệnh ở mỗi gia đình có thể được chứng minh. Các kiểu gen liên quan đến bệnh đã được xây dựng và cung cấp bằng chứng cho một người sáng lập tổ tiên cho các gia đình NCMD người Đức. Phân tích kiểu gen này gợi ý rằng một khoảng 4,0 cM được bao quanh bởi các dấu hiệu ở D6S249 và D6S475 chứa gen gây ra NCMD, tạo điều kiện cho các phương pháp nhân bản định vị thêm."} +{"idx": 1940030, "en": "Dominant optic atrophy, Kjer type, is an autosomal dominant disorder causing progressive loss of visual acuity and colour vision from early childhood. The gene (OPA1) has variable expressivity, a penetrance of 0.98, and the locus has been localised to 3q28-29. We have genotyped nine British families with the disease using 12 polymorphic microsatellite markers from this region. Linkage and haplotype analysis shows the OPA1 gene to be located in a 2.3 cM interval between markers D3S1601 and D3S2748. One family showed no evidence of linkage with the chromosome 3 markers, suggesting for the first time that locus heterogeneity for this disease may exist, although exclusion for linkage is based on unaffected subjects. In addition, analysis of recombinants has enabled us to order the 12 markers along chromosome 3.", "gemini": "Teo thần kinh thị giác trội, loại Kjer, là một rối loạn trội trên nhiễm sắc thể thường gây mất dần thị lực và thị lực màu từ thời thơ ấu. Gen (OPA1) có khả năng biểu hiện khác nhau, độ thấm là 0,98 và vị trí gen đã được xác định tại 3q28-29. Chúng tôi đã tạo kiểu gen cho chín gia đình người Anh mắc bệnh này bằng cách sử dụng 12 dấu hiệu đa hình vi vệ tinh từ vùng này. Phân tích liên kết và kiểu gen cho thấy gen OPA1 nằm trong khoảng 2,3 cM giữa các dấu hiệu D3S1601 và D3S2748. Một gia đình không có bằng chứng liên kết với các dấu hiệu nhiễm sắc thể 3, lần đầu tiên gợi ý rằng tính không đồng nhất về vị trí gen đối với bệnh này có thể tồn tại, mặc dù việc loại trừ liên kết dựa trên những đối tượng không bị ảnh hưởng. Ngoài ra, phân tích các thể tái tổ hợp đã cho phép chúng tôi sắp xếp 12 dấu hiệu dọc theo nhiễm sắc thể 3."} +{"idx": 1940031, "en": "BACKGROUND The autosomal recessive limb-girdle muscular dystrophies (LGMDs) are a group of genetically heterogeneous muscle diseases characterised by progressive proximal limb muscle weakness. Six different loci have been mapped and pathogenetic mutations in the genes encoding the sarcoglycan complex components (alpha-, beta-, gamma-, and delta-sarcoglycan) have been documented. LGMD patients affected with primary \"sarcoglycanopathies\" are classified as LGMD2D, 2E, 2C, and 2F, respectively. METHODS A geographical area in north east Italy (2,319,147 inhabitants) was selected for a genetic epidemiological study on primary sarcoglycanopathies. Within the period 1982 to 1996, all patients living in this region and diagnosed with muscular dystrophy were seen at our centre. Immunohistochemical and immunoblot screening for alpha-sarcoglycan protein deficiency was performed on all muscle biopsies from patients with a progressive muscular dystrophy of unknown aetiology and normal dystrophin. Sarcoglycan mutation analyses were conducted on all patient muscle biopsies shown to have complete or partial absence of alpha-sarcoglycan immunostaining or a decreased quantity of alpha-sarcoglycan protein on immunoblotting. RESULTS Two hundred and four patient muscle biopsies were screened for alpha-sarcoglycan protein deficiency and 18 biopsies showed a deficiency. Pathogenetic mutations involving one gene for sarcoglycan complex components were identified in 13 patients: alpha-sarcoglycan in seven, beta-sarcoglycan in two, gamma-sarcoglycan in four, and none in the delta-sarcoglycan gene. The overall prevalence of primary sarcoglycanopathies, as of 31 December 1996, was estimated to be 5.6 x 10(-6) inhabitants. CONCLUSION The prevalence rate estimated in this study is the first to be obtained after biochemical and molecular genetic screening for sarcoglycan defects.", "gemini": "BỐI CẢNH Các bệnh loạn dưỡng cơ vòng chi-vai (LGMD) lặn trên nhiễm sắc thể thường là một nhóm các bệnh cơ di truyền không đồng nhất có đặc điểm là yếu cơ chi gần tiến triển. Sáu vị trí gen khác nhau đã được lập bản đồ và các đột biến gây bệnh trong các gen mã hóa các thành phần phức hợp sarcoglycan (alpha-, beta-, gamma- và delta-sarcoglycan) đã được ghi nhận. Bệnh nhân LGMD bị ảnh hưởng bởi \"bệnh sarcoglycan\" chính được phân loại lần lượt là LGMD2D, 2E, 2C và 2F. PHƯƠNG PHÁP Một khu vực địa lý ở đông bắc Ý (2.319.147 cư dân) đã được chọn cho một nghiên cứu dịch tễ học di truyền về bệnh sarcoglycan chính. Trong giai đoạn từ năm 1982 đến năm 1996, tất cả bệnh nhân sống trong khu vực này và được chẩn đoán mắc bệnh loạn dưỡng cơ đều được khám tại trung tâm của chúng tôi. Đã tiến hành sàng lọc miễn dịch mô hóa học và miễn dịch chấm đối với tình trạng thiếu protein alpha-sarcoglycan trên tất cả các sinh thiết cơ từ những bệnh nhân mắc bệnh loạn dưỡng cơ tiến triển có nguyên nhân chưa rõ và dystrophin bình thường. Các phân tích đột biến sarcoglycan đã được tiến hành trên tất cả các sinh thiết cơ của bệnh nhân cho thấy tình trạng thiếu hoàn toàn hoặc một phần miễn dịch alpha-sarcoglycan hoặc lượng protein alpha-sarcoglycan giảm trên miễn dịch chấm. KẾT QUẢ Hai trăm bốn sinh thiết cơ của bệnh nhân đã được sàng lọc tình trạng thiếu protein alpha-sarcoglycan và 18 sinh thiết cho thấy tình trạng thiếu hụt. Đã xác định được các đột biến gây bệnh liên quan đến một gen đối với các thành phần phức hợp sarcoglycan ở 13 bệnh nhân: alpha-sarcoglycan ở bảy bệnh nhân, beta-sarcoglycan ở hai bệnh nhân, gamma-sarcoglycan ở bốn bệnh nhân và không có trường hợp nào ở gen delta-sarcoglycan. Tỷ lệ mắc bệnh sarcoglycan chính nói chung, tính đến ngày 31 tháng 12 năm 1996, ước tính là 5,6 x 10(-6) cư dân. KẾT LUẬN Tỷ lệ mắc bệnh ước tính trong nghiên cứu này là tỷ lệ đầu tiên thu được sau khi sàng lọc di truyền sinh hóa và phân tử đối với các khiếm khuyết sarcoglycan."} +{"idx": 1940032, "en": "Holt-Oram syndrome is an autosomal dominant disorder characterised by radial ray and congenital heart defects. Recently, a gene for this disorder has been identified on chromosome 12q24.1, encoding a T box transcription factor. However, the functional role of the gene product is not completely understood. We present results of neurological, radiological, and muscle magnetic resonance imaging (MRI) investigations in 13 patients from eight unrelated families. Besides heart defects, clinical signs ranged from thenar abnormalities to bilateral phocomelia. The former were present in all patients. MRI showed hypoplasia of discrete muscles which clinically showed as non-progressive weakness. The structural pattern of residual muscles was normal on MRI, which together with normal muscular power, electromyography, and muscle enzyme investigations excluded a progressive neuromuscular disorder. The number and location of hypoplastic muscles correlated with the severity of skeletal involvement. Thus, patients with hypoplasia of large and proximal muscles had phocomelia, and those with mere intrinsic hand muscle hypoplasia had only a triphalangeal thumb or no skeletal malformation. On the basis of these observations, we conclude that disturbed fetal limb muscle development is involved in the bony malformations of the upper limbs.", "gemini": "Hội chứng Holt-Oram là một rối loạn trội trên nhiễm sắc thể thường có đặc điểm là khiếm khuyết tia hướng tâm và tim bẩm sinh. Gần đây, một gen gây ra rối loạn này đã được xác định trên nhiễm sắc thể 12q24.1, mã hóa một yếu tố phiên mã hộp T. Tuy nhiên, vai trò chức năng của sản phẩm gen vẫn chưa được hiểu rõ hoàn toàn. Chúng tôi trình bày kết quả điều tra cộng hưởng từ (MRI) cơ, thần kinh và X quang ở 13 bệnh nhân từ tám gia đình không có quan hệ họ hàng. Bên cạnh các khiếm khuyết về tim, các dấu hiệu lâm sàng dao động từ bất thường thenar đến chứng phocomelia hai bên. Các bất thường trước đây có mặt ở tất cả các bệnh nhân. MRI cho thấy tình trạng kém phát triển của các cơ riêng biệt, biểu hiện lâm sàng là tình trạng yếu không tiến triển. Mẫu cấu trúc của các cơ còn lại là bình thường trên MRI, cùng với sức cơ bình thường, điện cơ đồ và các xét nghiệm men cơ loại trừ rối loạn thần kinh cơ tiến triển. Số lượng và vị trí của các cơ kém phát triển tương quan với mức độ nghiêm trọng của tình trạng tổn thương xương. Do đó, những bệnh nhân bị kém phát triển các cơ lớn và gần thì bị chứng phocomelia, còn những bệnh nhân chỉ bị kém phát triển cơ bàn tay nội tại thì chỉ bị ngón tay cái ba đốt hoặc không bị dị tật xương. Trên cơ sở những quan sát này, chúng tôi kết luận rằng sự phát triển bất thường của cơ chi thai nhi có liên quan đến các dị tật xương ở chi trên."} +{"idx": 1940033, "en": "In this study, the stress state occurring at each trabecular due to three different types of dental implant is investigated by a homogenization technique, in which the trabecular structure is assumed to be composed of repeating hexagonal units. This technique helps make a proper material model of bone and to analyse such a non-homogeneous structure at the level of an individual microstructural unit. Stress analyses with the homogenization technique show a much higher stress level in the sponge bone, compared to those with conventional FEM. It also shows that even a minor lateral force results in crucial stresses in the dental implant system. The stress states of the mandible with a hemisphere-rooted implant and a wedge type implant show similar levels, while those with a rectangular-rooted implant result in higher stresses. It is suggested that the distance between the implant tip and cortical bone be kept far enough apart to prevent stress concentrations in the mandible.", "gemini": "Trong nghiên cứu này, trạng thái ứng suất xảy ra tại mỗi xương xốp do ba loại implant nha khoa khác nhau được nghiên cứu bằng kỹ thuật đồng nhất hóa, trong đó cấu trúc xương xốp được coi là bao gồm các đơn vị hình lục giác lặp lại. Kỹ thuật này giúp tạo ra một mô hình vật liệu thích hợp của xương và phân tích cấu trúc không đồng nhất như vậy ở cấp độ của một đơn vị vi cấu trúc riêng lẻ. Các phân tích ứng suất với kỹ thuật đồng nhất hóa cho thấy mức ứng suất cao hơn nhiều ở xương xốp, so với các phân tích với FEM thông thường. Nó cũng cho thấy rằng ngay cả một lực bên nhỏ cũng dẫn đến các ứng suất quan trọng trong hệ thống implant nha khoa. Trạng thái ứng suất của xương hàm có implant gốc bán cầu và implant dạng nêm cho thấy các mức tương tự, trong khi các implant có gốc hình chữ nhật dẫn đến ứng suất cao hơn. Người ta cho rằng khoảng cách giữa đầu implant và xương vỏ phải được giữ đủ xa để ngăn ngừa tập trung ứng suất ở xương hàm."} +{"idx": 1940034, "en": "We report on five patients presenting with features of two congenital disorders, DiGeorge syndrome (DGS) and CHARGE association. CHARGE association is usually sporadic and its origin is as yet unknown. Conversely, more than 90% of DGS patients are monosomic for the 22q11.2 chromosomal region. In each of the five patients, both cytogenetic and molecular analysis for the 22q11.2 region were normal. In view of the broad clinical spectrum and the likely genetic heterogeneity of both disorders, these cases are consistent with the extended phenotype of either DGS without 22q11.2 deletion or CHARGE association, especially as several features of CHARGE association have been reported in rare patients with 22q11.2 deletion association phenotypes. On the other hand, these could be novel cases of an independent association involving a complex defect of neural crest cells originating from the pharyngeal pouches.", "gemini": "Chúng tôi báo cáo về năm bệnh nhân có các đặc điểm của hai rối loạn bẩm sinh, hội chứng DiGeorge (DGS) và hội chứng CHARGE. Hội chứng CHARGE thường xảy ra lẻ tẻ và nguồn gốc của nó vẫn chưa được biết. Ngược lại, hơn 90% bệnh nhân DGS bị đơn nhiễm sắc thể vùng 22q11.2. Ở mỗi trong năm bệnh nhân, cả phân tích tế bào di truyền và phân tử đối với vùng 22q11.2 đều bình thường. Theo quan điểm về phổ lâm sàng rộng và khả năng di truyền không đồng nhất của cả hai rối loạn, các trường hợp này phù hợp với kiểu hình mở rộng của DGS mà không có mất đoạn 22q11.2 hoặc hội chứng CHARGE, đặc biệt là vì một số đặc điểm của hội chứng CHARGE đã được báo cáo ở những bệnh nhân hiếm gặp có kiểu hình liên quan đến mất đoạn 22q11.2. Mặt khác, đây có thể là những trường hợp mới của một hội chứng độc lập liên quan đến khiếm khuyết phức tạp của các tế bào mào thần kinh có nguồn gốc từ các túi hầu."} +{"idx": 1940035, "en": "Accurate clinical diagnosis of the spinocerebellar ataxias (SCAs) can be difficult because of overlap in phenotype with other disorders and variation in clinical manifestations. Six SCA loci have been mapped and four disease causing genes identified, in addition to the causative gene for Friedreich's ataxia (FA). All of the identified mutations are expansions of trinucleotide repeat tracts. The SCA2 and SCA6 genes were published recently. The extent of the normal CAG size ranges at these loci and the relative frequencies of the known causes of SCA in the UK are not known. This study first investigated the normal size ranges of the SCA2 and SCA6 loci by genotyping control populations of West African and South African subjects, since African populations generally show the greatest allelic diversity. We found one allele larger than the previously determined normal range for SCA2, and our results at the SCA6 locus agreed with the previously reported normal range. The second component of the study assessed the relative frequencies of the SCA1, 2, 3, and 6, DRPLA, and FA trinucleotide repeat mutations in 146 patients presenting with SCA-like symptoms referred to genetic diagnostic laboratories in the UK. We detected mutations in 14% of patients referred with a diagnosis of autosomal dominant SCA, and in 15% of patients referred with spinocerebellar ataxia where we did not have sufficient family history data available to allow categorisation as familial or sporadic cases. Friedreich's ataxia accounted for 3% of the latter category of cases in our sample, but the most common causes of SCA were SCA2 and SCA6.", "gemini": "Chẩn đoán lâm sàng chính xác các chứng mất điều hòa tiểu não (SCA) có thể khó khăn vì sự chồng chéo về kiểu hình với các rối loạn khác và sự thay đổi trong các biểu hiện lâm sàng. Sáu vị trí SCA đã được lập bản đồ và bốn gen gây bệnh đã được xác định, ngoài gen gây bệnh cho chứng mất điều hòa của Friedreich (FA). Tất cả các đột biến được xác định đều là sự mở rộng của các đoạn lặp lại ba nucleotide. Các gen SCA2 và SCA6 đã được công bố gần đây. Mức độ phạm vi kích thước CAG bình thường tại các vị trí này và tần suất tương đối của các nguyên nhân đã biết của SCA ở Vương quốc Anh không được biết. Nghiên cứu này đầu tiên đã điều tra các phạm vi kích thước bình thường của các vị trí SCA2 và SCA6 bằng cách phân tích kiểu gen các quần thể đối chứng là người Tây Phi và Nam Phi, vì quần thể người châu Phi thường biểu hiện sự đa dạng về alen lớn nhất. Chúng tôi đã tìm thấy một alen lớn hơn phạm vi bình thường đã xác định trước đó đối với SCA2 và kết quả của chúng tôi tại vị trí SCA6 phù hợp với phạm vi bình thường đã báo cáo trước đó. Thành phần thứ hai của nghiên cứu đánh giá tần suất tương đối của các đột biến lặp lại ba nucleotide SCA1, 2, 3 và 6, DRPLA và FA ở 146 bệnh nhân có các triệu chứng giống SCA được giới thiệu đến các phòng xét nghiệm chẩn đoán di truyền tại Vương quốc Anh. Chúng tôi đã phát hiện ra các đột biến ở 14% bệnh nhân được giới thiệu với chẩn đoán SCA trội trên nhiễm sắc thể thường và ở 15% bệnh nhân được giới thiệu với chứng mất điều hòa tiểu não mà chúng tôi không có đủ dữ liệu tiền sử gia đình để cho phép phân loại là các trường hợp gia đình hoặc lẻ tẻ. Chứng mất điều hòa của Friedreich chiếm 3% trong số các trường hợp sau trong mẫu của chúng tôi, nhưng nguyên nhân phổ biến nhất của SCA là SCA2 và SCA6."} +{"idx": 1940036, "en": "Eighty-six women fulfilling specific selection criteria were studied for germline mutations in two breast cancer susceptibility genes, BRCA1 and BRCA2, using the protein truncation test (PTT). Nine germline mutations were identified, six in BRCA1 and three in BRCA2. Of the six BRCA1 mutations, three have previously been described and three are new, and for BRCA2, one is a new mutation and the other two appear to occur at a site that has been described several times. Four kindreds were breast cancer families, one a breast/ovarian cancer family, and the sixth an ovarian cancer family. The three kindreds with BRCA2 mutations were classified as one breast/ovarian cancer family, one breast cancer family, and one family which harboured one early onset breast cancer patient and two melanoma patients. The mutations in BRCA1 were either insertions, deletions, or transitions which all resulted in a premature stop codon. Mutations in BRCA2 were all frameshift mutations as a result of either 2 or 4 bp deletions. Two BRCA2 mutations were identical, suggesting a Swiss founder effect which was confirmed by haplotype sharing. The 10% mutation detection rate is compatible with the relaxed criteria used for patient selection. Considering the relative ease with which coding sequences can be screened by PTT, this assay is useful as a first screen for BRCA1 and BRCA2 mutations.", "gemini": "Tám mươi sáu phụ nữ đáp ứng các tiêu chuẩn tuyển chọn cụ thể đã được nghiên cứu về đột biến dòng mầm trong hai gen dễ mắc ung thư vú, BRCA1 và BRCA2, bằng cách sử dụng xét nghiệm cắt cụt protein (PTT). Chín đột biến dòng mầm đã được xác định, sáu đột biến ở BRCA1 và ba đột biến ở BRCA2. Trong số sáu đột biến BRCA1, ba đột biến đã được mô tả trước đây và ba đột biến mới, còn đối với BRCA2, một đột biến mới và hai đột biến khác dường như xảy ra tại một vị trí đã được mô tả nhiều lần. Bốn gia đình có quan hệ họ hàng là gia đình mắc ung thư vú, một gia đình mắc ung thư vú/buồng trứng và gia đình thứ sáu là gia đình mắc ung thư buồng trứng. Ba gia đình có quan hệ họ hàng với các đột biến BRCA2 được phân loại là một gia đình mắc ung thư vú/buồng trứng, một gia đình mắc ung thư vú và một gia đình có một bệnh nhân ung thư vú khởi phát sớm và hai bệnh nhân mắc bệnh u hắc tố. Các đột biến ở BRCA1 là đột biến chèn, xóa hoặc chuyển đổi, tất cả đều dẫn đến codon dừng sớm. Các đột biến ở BRCA2 đều là đột biến dịch khung do xóa 2 hoặc 4 bp. Hai đột biến BRCA2 giống hệt nhau, cho thấy hiệu ứng người sáng lập Thụy Sĩ đã được xác nhận bằng cách chia sẻ kiểu gen. Tỷ lệ phát hiện đột biến 10% tương thích với các tiêu chí nới lỏng được sử dụng để lựa chọn bệnh nhân. Xét đến việc dễ dàng sàng lọc trình tự mã hóa bằng PTT, xét nghiệm này hữu ích như một sàng lọc đầu tiên cho các đột biến BRCA1 và BRCA2."} +{"idx": 1940037, "en": "More than 600 different CFTR (cystic fibrosis transmembrane conductance regulator) gene mutations have been identified so far that are considered to cause the fatal genetic disorder cystic fibrosis (CF). We have investigated 15 Arab children from 12 families, who were diagnosed as having CF, for mutations in the coding region and in the flanking intron sequences of the CFTR gene. Six different CFTR mutations were identified including two novel mutations, 1548delG in exon 10 and 406-2A-->G in intron 3. Prominent mutations were the splice mutation 3120 + 1G-->A (intron 16) followed by N1303K (exon 21) and 1548delG (exon 10). Most CF children were homozygotes who presented with a severe form of the disease including failure to thrive, recurrent chest infections, particularly with Pseudomonas aeruginosa, and frequent hospital admissions. Identification of the CFTR mutations facilitates molecular investigation of the disease and better understanding of its pathophysiology in Arab children, among whom CF is probably an underdiagnosed disease.", "gemini": "Cho đến nay, người ta đã xác định được hơn 600 đột biến gen CFTR (chất điều hòa dẫn truyền xuyên màng xơ nang) khác nhau được coi là nguyên nhân gây ra bệnh xơ nang (CF) là một rối loạn di truyền gây tử vong. Chúng tôi đã nghiên cứu 15 trẻ em Ả Rập từ 12 gia đình, được chẩn đoán mắc bệnh CF, về các đột biến trong vùng mã hóa và trong các trình tự intron bao quanh của gen CFTR. Sáu đột biến CFTR khác nhau đã được xác định bao gồm hai đột biến mới, 1548delG ở exon 10 và 406-2A-->G ở intron 3. Các đột biến nổi bật là đột biến ghép nối 3120 + 1G-->A (intron 16) tiếp theo là N1303K (exon 21) và 1548delG (exon 10). Hầu hết trẻ em mắc bệnh CF là kiểu gen đồng hợp tử biểu hiện bệnh ở dạng nghiêm trọng bao gồm chậm phát triển, nhiễm trùng ngực tái phát, đặc biệt là do Pseudomonas aeruginosa và thường xuyên phải nhập viện. Việc xác định các đột biến CFTR tạo điều kiện cho việc điều tra phân tử về bệnh và hiểu rõ hơn về sinh lý bệnh của bệnh ở trẻ em Ả Rập, trong số đó CF có thể là một căn bệnh chưa được chẩn đoán."} +{"idx": 1940038, "en": "Intrafamilial marriage is favoured by the Arab community in Israel, almost all of whom live in villages populated by a few (< 20) founding families. A previous study in Taibe, a large Arab village located 30 km from Tel Aviv, showed a significantly high malformation rate among infants of consanguineous parents. The present study examines the reproductive consequences of parental consanguinity in 610 families from the same village, selected retrospectively through infants routinely seen in the local well baby clinic. All mothers were interviewed with regard to previous pregnancy outcomes, including abortions, stillbirths, and neonatal or infant deaths, as well as the degree of consanguinity. In addition, we analysed the anthropometric measurements of the probands. The incidence of infant deaths was significantly higher in the inbred group (p < 0.001). No significant increase in fetal loss between the inbred and outbred groups was observed. There were no differences in anthropometric features, except for a lower birth weight in the consanguineous group (p < 0.035). This study, combined with our previous studies of the same population, indicates a prominent public health problem associated with consanguineous marriage in the Arab community and a need for specific genetic counselling.", "gemini": "Hôn nhân cận huyết được ưa chuộng bởi cộng đồng người Ả Rập ở Israel, hầu hết đều sống ở các ngôi làng có vài (< 20) gia đình sáng lập. Một nghiên cứu trước đây ở Taibe, một ngôi làng Ả Rập lớn nằm cách Tel Aviv 30 km, cho thấy tỷ lệ dị tật ở trẻ sơ sinh của cha mẹ cận huyết cao đáng kể. Nghiên cứu hiện tại xem xét hậu quả sinh sản của cận huyết của cha mẹ trong 610 gia đình từ cùng một ngôi làng, được lựa chọn hồi cứu thông qua trẻ sơ sinh thường xuyên được thăm khám tại phòng khám trẻ khỏe địa phương. Tất cả các bà mẹ đều được phỏng vấn về kết quả mang thai trước đó, bao gồm phá thai, thai chết lưu và tử vong ở trẻ sơ sinh hoặc trẻ nhỏ, cũng như mức độ cận huyết. Ngoài ra, chúng tôi đã phân tích các phép đo nhân trắc học của những người được thử nghiệm. Tỷ lệ tử vong ở trẻ sơ sinh cao hơn đáng kể ở nhóm cận huyết (p < 0,001). Không có sự gia tăng đáng kể nào về mất thai giữa nhóm cận huyết và nhóm không cận huyết. Không có sự khác biệt về các đặc điểm nhân trắc học, ngoại trừ cân nặng khi sinh thấp hơn ở nhóm cận huyết (p < 0,035). Nghiên cứu này, kết hợp với các nghiên cứu trước đây của chúng tôi về cùng một quần thể, chỉ ra một vấn đề sức khỏe cộng đồng nổi bật liên quan đến hôn nhân cận huyết trong cộng đồng người Ả Rập và nhu cầu tư vấn di truyền cụ thể."} +{"idx": 1940039, "en": "Meckel syndrome (MKS) is a lethal, autosomal recessive condition characterised by an occipital meningoencephalocele, enlarged kidneys with multicystic dysplasia, fibrotic changes of the liver in the portal area with ductal proliferation, and postaxial polydactyly. Recently, a MKS gene has been mapped to chromosome 17q21-q24 in Finnish families, with no evidence of locus heterogeneity in this population. Here, we report the exclusion of chromosome 17q21-q24 in eight typical MKS families of North African and Middle Eastern ancestry and provide evidence for genetic heterogeneity of this condition.", "gemini": "Hội chứng Meckel (MKS) là một tình trạng lặn trên nhiễm sắc thể thường gây tử vong, đặc trưng bởi não úng thủy màng não chẩm, thận to với loạn sản đa nang, thay đổi xơ hóa của gan ở vùng cửa với sự tăng sinh ống dẫn và đa ngón sau trục. Gần đây, một gen MKS đã được lập bản đồ đến nhiễm sắc thể 17q21-q24 trong các gia đình Phần Lan, không có bằng chứng về tính không đồng nhất của vị trí trong quần thể này. Tại đây, chúng tôi báo cáo việc loại trừ nhiễm sắc thể 17q21-q24 trong tám gia đình MKS điển hình có tổ tiên Bắc Phi và Trung Đông và cung cấp bằng chứng về tính không đồng nhất về mặt di truyền của tình trạng này."} +{"idx": 1940040, "en": "Peutz-Jeghers syndrome (PJS, MIM 175,2000) is a disease of autosomal dominant inheritance that is characterised by hamartomatous gastrointestinal polyps and mucocutaneous pigmentation. In addition to problems such as intussusception, PJS predisposes to cancers of several sites. The unusual combination of clinical features makes the identification of the defect underlying PJS particularly interesting. Recently, the PJS gene has been mapped to chromosome 19p13.", "gemini": "Hội chứng Peutz-Jeghers (PJS, MIM 175.200) là một bệnh di truyền trội trên nhiễm sắc thể thường, đặc trưng bởi các polyp đường tiêu hóa dạng u hạt và sắc tố niêm mạc da. Ngoài các vấn đề như lồng ruột, PJS còn gây ung thư ở một số vị trí. Sự kết hợp bất thường của các đặc điểm lâm sàng khiến việc xác định khiếm khuyết cơ bản của PJS trở nên đặc biệt thú vị. Gần đây, gen PJS đã được lập bản đồ đến nhiễm sắc thể 19p13."} +{"idx": 1940041, "en": "A 7 year old boy with minor facial anomalies, the Rieger eye malformation, reduced vision, genital anomalies, and severe mental retardation had deletion of the segment 4q24-->q26. His phenotypically normal father had a balanced insertion of that segment into the distal long arm of chromosome 6: 46,XY,ins(6;4)(q26;q24q26). Microsatellite loci flanking the RIEG gene on 4q25 were deleted giving indirect evidence of deletion of this locus. This finding and the normal ocular findings in the insertion carrier father show that haplotype insufficiency can cause the Rieger eye malformation.", "gemini": "Một bé trai 7 tuổi có dị tật khuôn mặt nhẹ, dị tật mắt Rieger, giảm thị lực, dị tật sinh dục và chậm phát triển trí tuệ nghiêm trọng đã bị mất đoạn 4q24-->q26. Người cha có kiểu hình bình thường của cậu bé có một đoạn chèn cân bằng của đoạn đó vào nhánh dài xa của nhiễm sắc thể 6: 46,XY,ins(6;4)(q26;q24q26). Các vị trí vệ tinh vi thể bao quanh gen RIEG trên 4q25 đã bị xóa, cung cấp bằng chứng gián tiếp về việc xóa vị trí này. Phát hiện này và các phát hiện về mắt bình thường ở người cha mang đoạn chèn cho thấy rằng sự thiếu hụt kiểu gen có thể gây ra dị tật mắt Rieger."} +{"idx": 1940042, "en": "Non-syndromal, recessive deafness (NSRD) is the most common form of inherited deafness or hearing impairment in humans. NSRD is genetically heterogeneous and it has been estimated that as many as 35 different loci may be involved. We report the mapping of a novel locus for autosomal recessive, non-syndromal deafness (DFNB16) in three consanguineous families originating from Pakistan and the Middle East. Using multipoint analysis (HOMOZ/MAPMAKER) a maximum combined lod score of 6.5 was obtained for the interval D15S1039-D15S123. Recombination events and haplotype analysis define a 12-14 cM critical region between the markers D15S1039 and D15S155 on chromosome 15q15-q21.", "gemini": "Điếc không có hội chứng, lặn (NSRD) là dạng điếc hoặc suy giảm thính lực di truyền phổ biến nhất ở người. NSRD có tính dị hợp về mặt di truyền và người ta ước tính rằng có tới 35 vị trí khác nhau có thể liên quan. Chúng tôi báo cáo việc lập bản đồ một vị trí mới cho điếc không có hội chứng, lặn trên nhiễm sắc thể thường (DFNB16) trong ba gia đình cận huyết có nguồn gốc từ Pakistan và Trung Đông. Sử dụng phân tích đa điểm (HOMOZ/MAPMAKER), điểm lod kết hợp tối đa là 6,5 thu được cho khoảng D15S1039-D15S123. Các sự kiện tái tổ hợp và phân tích kiểu gen xác định một vùng tới hạn 12-14 cM giữa các dấu hiệu D15S1039 và D15S155 trên nhiễm sắc thể 15q15-q21."} +{"idx": 1940043, "en": "We report a mother and son who each presented in infancy with hypotonia and global developmental delay. Subsequently, in both subjects, mild mental handicap was diagnosed in association with temporal lobe arachnoid cysts. Mendelian inheritance of this phenotype seems likely and macroscopic cerebral dysplasia in general may be underdiagnosed in people with familial, mild mental handicap.", "gemini": "Chúng tôi báo cáo một người mẹ và con trai, mỗi người đều biểu hiện ở thời kỳ trẻ sơ sinh với tình trạng trương lực cơ thấp và chậm phát triển toàn diện. Sau đó, ở cả hai đối tượng, tình trạng khuyết tật trí tuệ nhẹ đã được chẩn đoán liên quan đến các nang nhện thùy thái dương. Sự di truyền theo kiểu Mendel của kiểu hình này có vẻ có khả năng và nhìn chung, chứng loạn sản não vĩ mô có thể chưa được chẩn đoán ở những người bị khuyết tật trí tuệ nhẹ trong gia đình."} +{"idx": 1940044, "en": "The cases of a brother and sister with dentato-olivary dysplasia are described. Both had severe developmental delay, severe epilepsy of early onset, evolving hypertonic quadriplegia, and death in early childhood. Postmortem examination of the brain in one child showed a particular form of dentato-olivary dysplasia. These children show many features in common with previously described cases of this condition, but this is the first report of occurrence in sibs.", "gemini": "Các trường hợp của một anh trai và chị gái bị loạn sản răng-olivary được mô tả. Cả hai đều chậm phát triển nghiêm trọng, động kinh nghiêm trọng khởi phát sớm, liệt tứ chi tăng trương lực tiến triển và tử vong ở thời thơ ấu. Khám nghiệm tử thi não ở một trẻ cho thấy một dạng loạn sản răng-olivary đặc biệt. Những đứa trẻ này có nhiều đặc điểm chung với các trường hợp mắc tình trạng này đã được mô tả trước đây, nhưng đây là báo cáo đầu tiên về sự xuất hiện ở anh chị em ruột."} +{"idx": 1940045, "en": "The role of various gene loci was investigated in a family in which familial osteoarthritis (FOA), with onset at an early age, is transmitted as an autosomal dominant mendelian trait. The absence of clinical and radiographic signs of dysplasia and calcium pyrophosphate deposition disease (CPDD) indicates that the basic disease process in this family is osteoarthritis (OA). Genetic linkage analysis of 14 candidate genes resulted in the exclusion of 10 important genes (COL2A1, COL9A1, COL9A2, COL11A1, COL11A2, COMP, the CPDD region, CRTL-1, CRTM, and MMP3). Other relevant genes were not informative in this family. The candidate loci previously identified in FOA and heritable skeletal disorders associated with OA are clearly not involved in the development of the primary FOA phenotype in the family investigated, indicating genetic heterogeneity.", "gemini": "Vai trò của nhiều gen khác nhau đã được nghiên cứu trong một gia đình mà bệnh thoái hóa khớp gia đình (FOA), khởi phát ở độ tuổi sớm, được di truyền như một tính trạng trội trên nhiễm sắc thể thường theo kiểu mendel. Việc không có các dấu hiệu lâm sàng và chụp X quang về loạn sản và bệnh lắng đọng canxi pyrophosphate (CPDD) chỉ ra rằng quá trình bệnh cơ bản trong gia đình này là bệnh thoái hóa khớp (OA). Phân tích liên kết di truyền của 14 gen ứng cử đã loại trừ 10 gen quan trọng (COL2A1, COL9A1, COL9A2, COL11A1, COL11A2, COMP, vùng CPDD, CRTL-1, CRTM và MMP3). Các gen có liên quan khác không cung cấp thông tin trong gia đình này. Các gen ứng cử được xác định trước đây ở FOA và các rối loạn xương di truyền liên quan đến OA rõ ràng không liên quan đến sự phát triển của kiểu hình FOA nguyên phát trong gia đình được nghiên cứu, chỉ ra tính không đồng nhất về mặt di truyền."} +{"idx": 1940046, "en": "X chromosome inactivation is widely studied using DNA sequence polymorphisms and DNA methylation as a surrogate measure of inactivation, but the correlation of methylation with inactivation is not perfect. Thus, it may be better to study sequence polymorphisms expressed in the mRNA. A recent paper reported use of a silent C/T polymorphism at nt 1311 of the G6PD cDNA, and this polymorphism was reported to have a frequency of 40% in all ethnic groups. We have screened 218 English and 50 Iranian subjects by PCR and restriction digestion; 53/218 (24%) British and 22/50 (44%) Iranian subjects were heterozygous. Thus, X inactivation studies using this polymorphism may be useful in some populations, including Iran, but much less so in the UK.", "gemini": "Việc bất hoạt nhiễm sắc thể X được nghiên cứu rộng rãi bằng cách sử dụng đa hình trình tự DNA và metyl hóa DNA như một biện pháp thay thế để bất hoạt, nhưng mối tương quan của metyl hóa với bất hoạt không hoàn hảo. Do đó, có thể tốt hơn là nghiên cứu đa hình trình tự được biểu hiện trong mRNA. Một bài báo gần đây đã báo cáo việc sử dụng đa hình C/T im lặng ở nt 1311 của cDNA G6PD và đa hình này được báo cáo có tần số 40% ở tất cả các nhóm dân tộc. Chúng tôi đã sàng lọc 218 đối tượng người Anh và 50 đối tượng người Iran bằng PCR và tiêu hóa hạn chế; 53/218 (24%) người Anh và 22/50 (44%) người Iran là dị hợp tử. Do đó, các nghiên cứu bất hoạt X sử dụng đa hình này có thể hữu ích ở một số quần thể, bao gồm cả Iran, nhưng ít hơn nhiều ở Vương quốc Anh."} +{"idx": 1940047, "en": "Gonadal dysgenesis resulting in primary infertility is one of the most common features of Turner syndrome. There have been a number of cases described of pregnancy in 45,X subjects, but whether or not the fertility is associated with a 46,XX cell line in the germ cells is not known. We describe a 45,X/46,X,psu idic(Xq) female with normal fertility, in whom a cryptic 46,XX cell line was found in the germ cells.", "gemini": "Suy sinh dục dẫn đến vô sinh nguyên phát là một trong những đặc điểm phổ biến nhất của hội chứng Turner. Đã có một số trường hợp mang thai được mô tả ở những đối tượng 45,X, nhưng liệu khả năng sinh sản có liên quan đến dòng tế bào 46,XX trong các tế bào mầm hay không thì vẫn chưa được biết. Chúng tôi mô tả một phụ nữ 45,X/46,X,psu idic(Xq) có khả năng sinh sản bình thường, trong đó một dòng tế bào 46,XX bí ẩn được tìm thấy trong các tế bào mầm."} +{"idx": 1940048, "en": "A healthy, non-consanguineous couple had a son with complete situs inversus viscerum (including dextrocardia but without other cardiac defects), broad thumbs and big toes, postaxial polydactyly, average intelligence and length proportion of the extremities, and a normal face. The common cause of these defects may have a role in the origin of sidedness and symmetry in morphogenesis.", "gemini": "Một cặp đôi khỏe mạnh, không cận huyết đã sinh ra một bé trai bị đảo ngược hoàn toàn nội tạng (bao gồm cả chứng lệch tim nhưng không có các khuyết tật tim khác), ngón tay cái và ngón chân cái rộng, đa ngón ở trục sau, trí thông minh trung bình và tỷ lệ chiều dài các chi, và khuôn mặt bình thường. Nguyên nhân chung của các khuyết tật này có thể có vai trò trong nguồn gốc của sự thuận bên và tính đối xứng trong quá trình hình thái."} +{"idx": 1940049, "en": "The major cause of the different forms of hyperphenylalaninaemia (HPA) is mutations in the gene encoding phenylalanine hydroxylase (PAH). The aim of this study was to determine the mutations responsible for mild forms of HPA and to relate different clinical phenotypes of HPA patients to their PAH genotypes. Four \"mild\" mutations, including the most frequent A403V and R297H mutations, occurred exclusively in mild hyperphenylalaninaemia (MHP). Mutations A104D, R243Q, R241H, and Y414C were detected in patients with mild phenylketonuria (mild PKU) only. These results may be useful in establishing a molecular differential diagnosis for PAH deficiency in Poland.", "gemini": "Nguyên nhân chính gây ra các dạng tăng phenylalanin máu khác nhau (HPA) là đột biến ở gen mã hóa phenylalanine hydroxylase (PAH). Mục đích của nghiên cứu này là xác định các đột biến chịu trách nhiệm cho các dạng HPA nhẹ và liên hệ các kiểu hình lâm sàng khác nhau của bệnh nhân HPA với kiểu gen PAH của họ. Bốn đột biến \"nhẹ\", bao gồm các đột biến A403V và R297H phổ biến nhất, chỉ xảy ra ở tăng phenylalanin máu nhẹ (MHP). Các đột biến A104D, R243Q, R241H và Y414C chỉ được phát hiện ở những bệnh nhân mắc phenylketon niệu nhẹ (PKU nhẹ). Những kết quả này có thể hữu ích trong việc thiết lập chẩn đoán phân biệt phân tử đối với tình trạng thiếu PAH ở Ba Lan."} +{"idx": 1940050, "en": "Recent studies with transgenic animals have considerably advanced our knowledge of the roles of dystrophin and utrophin in both muscle and non-muscle tissues. Rigorous analyses of the roles of the various mdx mutations in mice, as well as the use of artificial transgenes in an mdx background, are beginning to define the functional importance of various regions of the dystrophin protein in normal muscle. Furthermore, recent biochemical analyses have revealed new insights into the role and organization of dystrophin at the membrane-cytoskeleton interface. Transgenic approaches have also revealed surprising and encouraging results with respect to utrophin. Against expectations, the long-awaited utrophin knockout mice have a remarkably mild phenotype with only subtle changes in neuromuscular junction architecture. On the other hand, mdx mice transgenic for a mini-utrophin construct showed rescue of the muscular dystrophy phenotype, clearly an encouraging finding with obvious therapeutic possibilities. These and other recent findings are discussed in the context of the structure and function of dystrophin and utrophin at the membrane-cytoskeleton interface.", "gemini": "Các nghiên cứu gần đây với động vật chuyển gen đã nâng cao đáng kể kiến thức của chúng ta về vai trò của dystrophin và utrophin trong cả mô cơ và mô không phải cơ. Các phân tích nghiêm ngặt về vai trò của các đột biến mdx khác nhau ở chuột, cũng như việc sử dụng các transgen nhân tạo trong nền mdx, đang bắt đầu xác định tầm quan trọng chức năng của các vùng khác nhau của protein dystrophin trong cơ bình thường. Hơn nữa, các phân tích sinh hóa gần đây đã tiết lộ những hiểu biết mới về vai trò và tổ chức của dystrophin tại giao diện màng-khung xương. Các phương pháp tiếp cận chuyển gen cũng đã tiết lộ những kết quả đáng ngạc nhiên và đáng khích lệ đối với utrophin. Trái với mong đợi, những con chuột loại gen utrophin được chờ đợi từ lâu có kiểu hình nhẹ đáng kể với chỉ những thay đổi nhỏ trong cấu trúc tiếp giáp thần kinh cơ. Mặt khác, những con chuột mdx chuyển gen cho một cấu trúc utrophin nhỏ đã cho thấy sự phục hồi kiểu hình loạn dưỡng cơ, rõ ràng là một phát hiện đáng khích lệ với các khả năng điều trị rõ ràng. Những phát hiện này và các phát hiện gần đây khác được thảo luận trong bối cảnh cấu trúc và chức năng của dystrophin và utrophin tại giao diện màng-khung xương."} +{"idx": 1940051, "en": "The purpose of this study was to investigate the initiation and time course of the regeneration process in fragments of skeletal muscle transplants as a function of muscle tissue age at implantation. The appearance of desmin occurs at the very beginning of myogenesis. The transgenic desmin nls lacZ mice used in the study bear a transgene in which the 1 kb DNA 5' regulatory sequence of the desmin gene is linked to a reporter gene coding for Escherichia coli beta-galactosidase. The desmin lacZ transgene labels muscle cells in which the desmin synthesis programme has commenced. We implanted pectoralis muscle fragments from fetal transgenic embryos and mature and old transgenic mice into mature non-transgenic mice. Early events of myogenesis occurring during regeneration started sooner in transplants from 4-month-old (day 3 post-implantation) muscle than in those from 24-month-old (day 5-6 post-implantation) muscle, and they lasted longer in those from young (day 17 post-implantation) than in those from old (day 14 post-implantation) muscle fragments. In adult muscle, transgene activation proceeded from the periphery toward the centre of the transplant. In transplants from fetal 18-day-old pectoralis, myotubes with transgene activity were observed from day 1 to day 19. Desmin immunoreactivity, which appeared about one day after transgene activation, was followed by myosin expression. In adult transplants, the continuity of laminin labelling was disrupted around degenerative fibres, illustrating alteration of the extracellular matrix. Our data suggest that satellite cells from old muscle tissue have lower proliferative capacity and/or less access to trophic substances released by the host (damaged fibres, vascularization) than those from fetal or young adult muscle.", "gemini": "Mục đích của nghiên cứu này là để tìm hiểu khởi đầu và tiến trình thời gian của quá trình tái sinh trong các mảnh ghép cơ xương như một chức năng của độ tuổi mô cơ khi cấy ghép. Sự xuất hiện của desmin xảy ra ngay từ đầu của quá trình sinh cơ. Chuột desmin nls lacZ chuyển gen được sử dụng trong nghiên cứu mang một gen chuyển gen trong đó trình tự điều hòa 1 kb DNA 5' của gen desmin được liên kết với một gen báo cáo mã hóa cho Escherichia coli beta-galactosidase. Gen chuyển gen desmin lacZ đánh dấu các tế bào cơ mà chương trình tổng hợp desmin đã bắt đầu. Chúng tôi đã cấy ghép các mảnh cơ ngực từ phôi chuyển gen và chuột chuyển gen trưởng thành và già vào chuột không chuyển gen trưởng thành. Các sự kiện sớm của quá trình sinh cơ xảy ra trong quá trình tái sinh bắt đầu sớm hơn ở các mảnh ghép từ cơ 4 tháng tuổi (ngày thứ 3 sau khi cấy ghép) so với cơ 24 tháng tuổi (ngày thứ 5-6 sau khi cấy ghép) và chúng kéo dài lâu hơn ở cơ trẻ (ngày 17 sau khi cấy ghép) so với cơ già (ngày 14 sau khi cấy ghép). Ở cơ trưởng thành, hoạt hóa gen chuyển gen tiến triển từ ngoại biên về phía trung tâm của mảnh ghép. Ở các mảnh ghép từ cơ ngực phôi 18 ngày tuổi, các ống cơ có hoạt tính gen chuyển gen được quan sát thấy từ ngày thứ 1 đến ngày thứ 19. Miễn dịch phản ứng desmin, xuất hiện khoảng một ngày sau khi hoạt hóa gen chuyển gen, tiếp theo là biểu hiện của myosin. Ở các mảnh ghép trưởng thành, tính liên tục của nhãn laminin bị phá vỡ xung quanh các sợi thoái hóa, minh họa sự thay đổi của ma trận ngoại bào. Dữ liệu của chúng tôi cho thấy các tế bào vệ tinh từ mô cơ già có khả năng sinh sản thấp hơn và/hoặc ít tiếp cận hơn với các chất dinh dưỡng do vật chủ giải phóng (các sợi bị tổn thương, mạch máu) so với cơ của thai nhi hoặc cơ trưởng thành trẻ."} +{"idx": 1940052, "en": "A unique property of smooth muscle is its ability to maintain force with a very low expenditure of energy. This characteristic is highly expressed in molluscan smooth muscles, such as the anterior byssus retractor muscle (ABRM) of Mytilus edulis, during a contractile state called 'catch'. Catch occurs following the initial activation of the muscle, and is characterized by prolonged force maintenance in the face of a low [Ca2+]i, high instantaneous stiffness, a very slow cross-bridge cycling rate, and low ATP usage. In the intact muscle, rapid relaxation (release of catch) is initiated by serotonin, and mediated by an increase in cAMP and activation of protein kinase A. We sought to determine which proteins undergo a change in phosphorylation on a time-course that corresponds to the release of catch in permeabilized ABRM. Only one protein consistently satisfied this criterion. This protein, having a molecular weight of approximately 600 kDa and a molar concentration about 30 times lower than the myosin heavy chain, showed an increase in phosphorylation during the release of catch. Under the mechanical conditions studied (rest, activation, catch, and release of catch), changes in phosphorylation of all other proteins, including myosin light chains, myosin heavy chain and paramyosin, are minimal compared with the cAMP-induced phosphorylation of the approximately 600 kDa protein. Under these conditions, somewhat less than one mole of phosphate is incorporated per mole of approximately 600 kDa protein. Inhibition of A kinase blocked both the cAMP-induced increase in phosphorylation of the protein and the release of catch. In addition, irreversible thiophosphorylation of the protein prevented the development of catch. In intact muscle, the degree of phosphorylation of the protein increases significantly when catch is released with serotonin. In muscles pre-treated with serotonin, a net dephosphorylation of the protein occurs when the muscle is subsequently put into catch. We conclude that the phosphorylation state of the approximately 600 kDa protein regulates catch.", "gemini": "Một đặc tính độc đáo của cơ trơn là khả năng duy trì lực với mức tiêu hao năng lượng rất thấp. Đặc điểm này được biểu hiện rõ ở cơ trơn của động vật thân mềm, chẳng hạn như cơ co cơ byssus trước (ABRM) của Mytilus edulis, trong trạng thái co được gọi là 'bắt'. Sự bắt xảy ra sau khi cơ được kích hoạt ban đầu và được đặc trưng bởi việc duy trì lực kéo dài khi đối mặt với [Ca2+]i thấp, độ cứng tức thời cao, tốc độ chu kỳ cầu nối chéo rất chậm và sử dụng ATP thấp. Ở cơ còn nguyên vẹn, sự giãn cơ nhanh chóng (giải phóng sự bắt) được khởi đầu bởi serotonin và được trung gian bởi sự gia tăng cAMP và hoạt hóa protein kinase A. Chúng tôi tìm cách xác định xem protein nào trải qua sự thay đổi về phosphoryl hóa theo tiến trình thời gian tương ứng với sự giải phóng sự bắt ở ABRM đã thấm. Chỉ có một protein liên tục đáp ứng tiêu chí này. Protein này, có trọng lượng phân tử khoảng 600 kDa và nồng độ mol thấp hơn khoảng 30 lần so với chuỗi nặng myosin, cho thấy sự gia tăng phosphoryl hóa trong quá trình giải phóng sự bắt. Trong các điều kiện cơ học được nghiên cứu (nghỉ ngơi, kích hoạt, bắt và giải phóng sự bắt), những thay đổi về phosphoryl hóa của tất cả các protein khác, bao gồm cả chuỗi nhẹ myosin, chuỗi nặng myosin và paramyosin, là tối thiểu so với phosphoryl hóa do cAMP gây ra của protein khoảng 600 kDa. Trong những điều kiện này, ít hơn một mol phosphat được kết hợp trên mỗi mol protein khoảng 600 kDa. Sự ức chế A kinase đã ngăn chặn cả sự gia tăng phosphoryl hóa của protein do cAMP gây ra và sự giải phóng sự bắt. Ngoài ra, sự thiophosphoryl hóa không thể đảo ngược của protein đã ngăn chặn sự phát triển của sự bắt. Ở cơ còn nguyên vẹn, mức độ phosphoryl hóa của protein tăng lên đáng kể khi sự bắt được giải phóng bằng serotonin. Ở những cơ được xử lý trước bằng serotonin, sự khử phosphoryl hóa ròng của protein xảy ra khi cơ sau đó bị bắt. Chúng tôi kết luận rằng trạng thái phosphoryl hóa của protein khoảng 600 kDa điều chỉnh sự bắt."} +{"idx": 1940053, "en": "To explain observed differences in the activation dependence of force redevelopment kinetics between cardiac and skeletal muscle, two numerical models of contractile regulation by Ca2+ were investigated. Ca2+ binding and force production were each modelled as two-state processes with forward and reverse rate constants taken from the literature. The first model incorporates four possible thin-filament states. In the second model Ca2+ is assumed not to dissociate from a thin-filament unit in the force-generating state, resulting in three states. The four-state model can account for the activation dependence of the rate constant of tension redevelopment (ktr) seen in skeletal muscle, without requiring that Ca2+ directly modulates the kinetics of any step in the cross-bridge cycle. Using identical kinetic parameters, the three-state model shows no activation dependence of ktr, consistent with our results in cardiac muscle. Following a step increase in [Ca2+], the rate of rise in tension (as described by the rate constant kCa) varies with the final [Ca2+] for both models, consistent with experimental results from skeletal and cardiac muscle. These numerical models demonstrate that experimental measurements thought to reveal changes in kinetic parameters may simply reflect coupling between the two kinetic processes of Ca2+ binding and force generation. Furthermore, the models present possible differences in the Ca2+ activation scheme between cardiac and skeletal muscle which can account for the contrasting activation dependencies of force redevelopment kinetics.", "gemini": "Để giải thích sự khác biệt quan sát được trong sự phụ thuộc vào hoạt hóa của động học tái phát triển lực giữa cơ tim và cơ xương, hai mô hình số của sự điều chỉnh co cơ bởi Ca2+ đã được nghiên cứu. Sự liên kết Ca2+ và sản xuất lực đều được mô hình hóa như các quá trình hai trạng thái với các hằng số tốc độ tiến và lùi lấy từ tài liệu. Mô hình đầu tiên kết hợp bốn trạng thái sợi mỏng có thể xảy ra. Trong mô hình thứ hai, Ca2+ được cho là không phân ly khỏi một đơn vị sợi mỏng ở trạng thái tạo lực, dẫn đến ba trạng thái. Mô hình bốn trạng thái có thể giải thích cho sự phụ thuộc vào hoạt hóa của hằng số tốc độ tái phát triển lực căng (ktr) được thấy ở cơ xương, mà không yêu cầu Ca2+ điều chỉnh trực tiếp động học của bất kỳ bước nào trong chu kỳ cầu nối chéo. Sử dụng các thông số động học giống hệt nhau, mô hình ba trạng thái không cho thấy sự phụ thuộc vào hoạt hóa của ktr, phù hợp với kết quả của chúng tôi ở cơ tim. Sau khi tăng bước trong [Ca2+], tốc độ tăng lực căng (như được mô tả bởi hằng số tốc độ kCa) thay đổi theo [Ca2+] cuối cùng cho cả hai mô hình, phù hợp với kết quả thực nghiệm từ cơ xương và cơ tim. Các mô hình số này chứng minh rằng các phép đo thực nghiệm được cho là tiết lộ những thay đổi về các thông số động học có thể chỉ đơn giản phản ánh sự ghép nối giữa hai quá trình động học là liên kết Ca2+ và tạo lực. Hơn nữa, các mô hình đưa ra những sự khác biệt có thể xảy ra trong lược đồ hoạt hóa Ca2+ giữa cơ tim và cơ xương có thể giải thích cho sự phụ thuộc vào hoạt hóa trái ngược nhau của động học tái phát triển lực."} +{"idx": 1940054, "en": "The effects of long lasting (4-5 weeks) nerve conduction block and denervation were compared by investigating contractile, morphological and histochemical properties of slow (soleus) and fast (EDL) rat skeletal muscles. The block was based on improved perfusion techniques of the sciatic nerve with a tetrodotoxin (TTX) solution delivered at doses adequate to obtain maximal effects in the muscles. The TTX-inactivated axons retained normal histological and physiological properties such as the ability to evoke full contractile responses, to regenerate, and to completely reinnervate muscle. In spite of their intact innervation or of their full reinnervation, the TTX-paralysed muscles underwent weight loss, fibre atrophy and reduction in force, output quantitatively indistinguishable from those following denervation. The same was true for all other contractile parameters tested, that is, twitch speed, twitch to tetanus ratio, post-tetanic potentiation, endurance, and fibre type composition. The results indicate the fundamental role of activity as a regulatory signal for muscle contractile properties, while they do not support the notion of a participation of chemical, activity-independent factors in this regulation.", "gemini": "Tác dụng của sự tắc nghẽn dẫn truyền thần kinh kéo dài (4-5 tuần) và sự hủy thần kinh đã được so sánh bằng cách nghiên cứu các đặc tính co bóp, hình thái và hóa mô học của các cơ xương chậm (cơ soleus) và nhanh (cơ EDL) của chuột. Sự tắc nghẽn dựa trên các kỹ thuật tưới máu được cải thiện của dây thần kinh hông bằng dung dịch tetrodotoxin (TTX) được cung cấp với liều lượng đủ để đạt được hiệu quả tối đa ở các cơ. Các sợi trục bị bất hoạt bởi TTX vẫn giữ được các đặc tính mô học và sinh lý bình thường như khả năng gây ra phản ứng co bóp hoàn toàn, khả năng tái sinh và khả năng phục hồi hoàn toàn cơ. Mặc dù sự chi phối thần kinh còn nguyên vẹn hoặc sự phục hồi thần kinh hoàn toàn, các cơ bị tê liệt do TTX vẫn bị giảm cân, teo cơ và giảm lực, về mặt số lượng không thể phân biệt được với những cơ bị hủy thần kinh. Điều tương tự cũng đúng đối với tất cả các thông số co bóp khác đã được kiểm tra, tức là tốc độ co giật, tỷ lệ co giật với co cứng, tăng cường sau co cứng, sức bền và thành phần loại sợi. Các kết quả cho thấy vai trò cơ bản của hoạt động như một tín hiệu điều chỉnh đối với các đặc tính co bóp của cơ, trong khi chúng không ủng hộ quan điểm cho rằng các yếu tố không phụ thuộc vào hoạt động, hóa học có sự tham gia vào quá trình điều chỉnh này."} +{"idx": 1940055, "en": "Using anion-exchange fast protein liquid chromatography, 14S dynein was separated into four fractions (designated 1-4). These fractions were distinguished with respect to polypeptide composition, and at least four unique heavy chains were identified. Each fraction was shown to exhibit ATPase activity. Fraction 2 has a specific activity 2-3 times greater than that of fractions 1, 3, and 4; the fractions showed a consistent trend of decreasing activity in the order 2 > 3 > 1 > 4. In all cases, the specific ATPase activity was reduced by high ionic strength, in contrast to 22S dynein, which was previously shown to exhibit increased activity under identical conditions. Electron microscopy analysis revealed that the four fractions of 14S dynein were structurally distinct. Fraction 1 comprises two globular head domains interconnected via two stems; fraction 2 consists of at least two clearly different globular structures; fraction 3 is a single globular head; and fraction 4 comprises three globular head domains interconnected by three stems to a basal structure. Further structural characterization using hydrodynamic techniques enabled a determination of mass and sedimentation coefficient for each fraction. Fraction 1 had a mass of 654 kDa and a sedimentation coefficient of 20.1 S. Fraction 2 had a variable mass due to association (616-966 kDa), and a sedimentation coefficient of 16.6 S, whereas fractions 3 and 4 had variable sedimentation coefficients but were of mass 701 kDa and 527 kDa respectively. Where possible, hydrodynamic parameters were utilized, in conjunction with electron microscopy data, to construct low-resolution hydrodynamic bead models to represent the fractions. Optimal models, which were consistent with all the available data, were produced for fractions 1 and 4. Bead modelling was also carried out for 22S dynein, using previously published data, to validate the 14S dynein modelling.", "gemini": "Sử dụng sắc ký lỏng protein nhanh trao đổi anion, dynein 14S được tách thành bốn phân đoạn (được chỉ định là 1-4). Các phân đoạn này được phân biệt về thành phần polypeptide và ít nhất bốn chuỗi nặng duy nhất đã được xác định. Mỗi phân đoạn đã được chứng minh là biểu hiện hoạt tính ATPase. Phân đoạn 2 có hoạt tính riêng gấp 2-3 lần so với phân đoạn 1, 3 và 4; các phân đoạn cho thấy xu hướng giảm hoạt động theo thứ tự 2 > 3 > 1 > 4. Trong mọi trường hợp, hoạt tính ATPase riêng biệt đã giảm do nồng độ ion cao, trái ngược với dynein 22S, trước đây đã được chứng minh là biểu hiện hoạt động tăng lên trong điều kiện giống hệt nhau. Phân tích kính hiển vi điện tử cho thấy bốn phân đoạn của dynein 14S có cấu trúc riêng biệt. Phân đoạn 1 bao gồm hai miền đầu hình cầu được kết nối với nhau thông qua hai thân; phân đoạn 2 bao gồm ít nhất hai cấu trúc hình cầu rõ ràng khác nhau; phân đoạn 3 là một đầu hình cầu đơn; và phân đoạn 4 bao gồm ba miền đầu hình cầu được kết nối với nhau bằng ba thân với một cấu trúc cơ bản. Đặc điểm cấu trúc sâu hơn bằng cách sử dụng các kỹ thuật thủy động lực học cho phép xác định khối lượng và hệ số lắng đọng cho mỗi phân đoạn. Phân đoạn 1 có khối lượng 654 kDa và hệ số lắng đọng là 20,1 S. Phân đoạn 2 có khối lượng thay đổi do liên kết (616-966 kDa) và hệ số lắng đọng là 16,6 S, trong khi phân đoạn 3 và 4 có hệ số lắng đọng thay đổi nhưng có khối lượng lần lượt là 701 kDa và 527 kDa. Khi có thể, các thông số thủy động lực học đã được sử dụng kết hợp với dữ liệu kính hiển vi điện tử để xây dựng các mô hình hạt thủy động lực học có độ phân giải thấp để biểu diễn các phân đoạn. Các mô hình tối ưu, phù hợp với tất cả các dữ liệu có sẵn, đã được tạo ra cho các phân đoạn 1 và 4. Mô hình hạt cũng được thực hiện đối với dynein 22S, sử dụng dữ liệu đã công bố trước đó, để xác thực mô hình dynein 14S."} +{"idx": 1940056, "en": "This case-control study assessed whether office workers who report more severe levels of musculoskeletal symptoms of the upper extremities demonstrate higher levels of keyforce in comparison to controls with less severe symptoms. Office workers reporting working on computer keyboards for four hours per day were classified as cases or controls based upon a median split on a Composite Symptom Severity score (cases = 23, controls = 25). Keyboard force and keying rate were measured during a 15-minute keyboarding task. Measures of task-related discomfort, muscular fatigue, pain, upper extremity symptoms, psychological distress and force were collected at baseline, post-keyboard task, and recovery. Ratings of perceived effort and task credibility were also obtained. Measures of work demands, perceived job stress, and upper extremity strength and flexibility were also collected. The results indicated group equivalence on reported work demands and upper extremity strength. Cases were more likely to receive a medical diagnosis of upper extremity cumulative trauma disorder, awaken from sleep due to symptoms, report higher levels of pain during work, experience greater impact of pain on function, and report higher workload pressure and lower support. Cases generated significantly higher keyboarding forces than controls, although both groups produced forces well above that required to operate the keyboard (4-5 times activation force). Cases reported higher levels of upper extremity symptoms and discomfort than controls, and these measures were highest after the keyboarding task for both groups. No significant correlation between keyforce and key rate was observed in either group. Results suggest that generation of excessive force while working on a computer keyboard may contribute to the severity of upper extremity symptoms. Clinically, the findings suggest that evaluating how an individual worker performs keyboarding tasks, or his or her workstyle, may be helpful in the management of these symptoms and disorders.", "gemini": "Nghiên cứu đối chứng trường hợp này đánh giá xem những nhân viên văn phòng báo cáo mức độ nghiêm trọng hơn của các triệu chứng cơ xương ở các chi trên có biểu hiện mức độ lực phím cao hơn so với nhóm đối chứng có các triệu chứng ít nghiêm trọng hơn hay không. Những nhân viên văn phòng báo cáo làm việc trên bàn phím máy tính bốn giờ mỗi ngày được phân loại là trường hợp hoặc đối chứng dựa trên điểm chia trung bình về điểm số mức độ nghiêm trọng của triệu chứng tổng hợp (trường hợp = 23, đối chứng = 25). Lực phím và tốc độ gõ phím được đo trong một tác vụ gõ phím 15 phút. Các biện pháp về khó chịu liên quan đến công việc, mệt mỏi cơ bắp, đau, các triệu chứng ở chi trên, đau khổ về mặt tâm lý và lực đã được thu thập khi bắt đầu, sau khi thực hiện tác vụ gõ phím và phục hồi. Xếp hạng về nỗ lực nhận thức và độ tin cậy của nhiệm vụ cũng được thu thập. Các biện pháp về nhu cầu công việc, căng thẳng công việc được nhận thức và sức mạnh và tính linh hoạt của chi trên cũng được thu thập. Kết quả cho thấy sự tương đương của nhóm về nhu cầu công việc được báo cáo và sức mạnh của chi trên. Các trường hợp có nhiều khả năng được chẩn đoán mắc chứng rối loạn chấn thương tích lũy ở chi trên, thức giấc khỏi giấc ngủ do các triệu chứng, báo cáo mức độ đau cao hơn trong khi làm việc, trải nghiệm tác động lớn hơn của cơn đau đối với chức năng và báo cáo áp lực khối lượng công việc cao hơn và sự hỗ trợ thấp hơn. Các trường hợp tạo ra lực gõ phím cao hơn đáng kể so với nhóm đối chứng, mặc dù cả hai nhóm đều tạo ra lực cao hơn nhiều so với lực cần thiết để vận hành bàn phím (lực kích hoạt gấp 4-5 lần). Các trường hợp báo cáo mức độ các triệu chứng và khó chịu ở chi trên cao hơn so với nhóm đối chứng và các biện pháp này cao nhất sau khi thực hiện tác vụ gõ phím đối với cả hai nhóm. Không thấy có mối tương quan đáng kể nào giữa lực phím và tốc độ phím ở cả hai nhóm. Kết quả cho thấy rằng việc tạo ra lực quá mức khi làm việc trên bàn phím máy tính có thể góp phần vào mức độ nghiêm trọng của các triệu chứng ở chi trên. Về mặt lâm sàng, những phát hiện này cho thấy rằng việc đánh giá cách một nhân viên thực hiện các tác vụ gõ phím hoặc phong cách làm việc của họ có thể hữu ích trong việc quản lý các triệu chứng và rối loạn này."} +{"idx": 1940057, "en": "This paper is a critical review of the clinical and cost outcome evaluation studies of multifactorial, comprehensive, cardiovascular risk management programs in worksites. A comprehensive international literature search conducted under the auspices of the National Heart, Lung and Blood Institute identified 17 articles based on 12 studies that examined the clinical outcomes of multifactorial, comprehensive programs. These articles were identified through MEDLINE, manual searches of recent journals, and through direct inquiries to worksite health promotion researchers. All studies were conducted between 1978 and 1995, with 1978 being the date of the first citation of a methodologically rigorous evaluation. Of the 12 research studies, only 8 utilized the worksite as both the unit of assignment and as the unit of analysis. None of the studies analyzed adequately for cost effectiveness. Given this limitation, this review briefly considers the relevant worksite research that has demonstrated cost outcomes. Worksite-based, multifactorial cardiovascular intervention programs reviewed for this article varied widely in the comprehensiveness, intensity, and duration of both the interventions and evaluations. Results from randomized trials suggest that providing opportunities for individualized, cardiovascular risk reduction counseling for high-risk employees within the context of comprehensive programming may be the critical component of an effective worksite intervention. Despite the many limitations of the current methodologies of the 12 studies, the majority of the research to date indicates the following: (1) favorable clinical and cost outcomes; (2) that more recent and more rigorously designed research tends to support rather than refute earlier and less rigorously designed studies; and (3) that rather than interpreting the methodological flaws and diversity as inherently negative, one may consider it as indicative of a robust phenomena evident in many types of worksites, with diverse employees, differing interventions, and varying degrees of methodological sophistication. Results of these studies reviewed provide both cautious optimism about the effectiveness of these worksite programs and insights regarding the essential components and characteristics of successful programs.", "gemini": "Bài báo này là một đánh giá phê bình về các nghiên cứu đánh giá kết quả lâm sàng và chi phí của các chương trình quản lý rủi ro tim mạch toàn diện, đa yếu tố tại nơi làm việc. Một cuộc tìm kiếm tài liệu quốc tế toàn diện được tiến hành dưới sự bảo trợ của Viện Tim, Phổi và Máu Quốc gia đã xác định được 17 bài báo dựa trên 12 nghiên cứu đã kiểm tra các kết quả lâm sàng của các chương trình toàn diện, đa yếu tố. Các bài báo này được xác định thông qua MEDLINE, tìm kiếm thủ công các tạp chí gần đây và thông qua các cuộc điều tra trực tiếp với các nhà nghiên cứu về thúc đẩy sức khỏe tại nơi làm việc. Tất cả các nghiên cứu đều được tiến hành trong khoảng thời gian từ năm 1978 đến năm 1995, với năm 1978 là ngày trích dẫn đầu tiên của một đánh giá có phương pháp nghiêm ngặt. Trong số 12 nghiên cứu, chỉ có 8 nghiên cứu sử dụng nơi làm việc làm đơn vị chỉ định và đơn vị phân tích. Không có nghiên cứu nào phân tích đầy đủ về hiệu quả chi phí. Với hạn chế này, bài đánh giá này xem xét một cách ngắn gọn các nghiên cứu về nơi làm việc có liên quan đã chứng minh được kết quả về chi phí. Các chương trình can thiệp tim mạch đa yếu tố tại nơi làm việc được xem xét cho bài báo này có sự khác biệt rất lớn về tính toàn diện, cường độ và thời gian của cả các can thiệp và đánh giá. Kết quả từ các thử nghiệm ngẫu nhiên cho thấy rằng việc cung cấp các cơ hội cho dịch vụ tư vấn giảm nguy cơ tim mạch cá nhân cho những nhân viên có nguy cơ cao trong bối cảnh lập trình toàn diện có thể là thành phần quan trọng của một biện pháp can thiệp hiệu quả tại nơi làm việc. Mặc dù có nhiều hạn chế về phương pháp luận hiện tại của 12 nghiên cứu, phần lớn các nghiên cứu cho đến nay chỉ ra những điều sau: (1) kết quả lâm sàng và chi phí thuận lợi; (2) rằng các nghiên cứu gần đây hơn và được thiết kế chặt chẽ hơn có xu hướng hỗ trợ hơn là bác bỏ các nghiên cứu trước đó và được thiết kế ít chặt chẽ hơn; và (3) rằng thay vì diễn giải các sai sót về phương pháp luận và sự đa dạng là tiêu cực, người ta có thể coi đó là dấu hiệu của một hiện tượng mạnh mẽ thể hiện ở nhiều loại nơi làm việc, với nhiều nhân viên khác nhau, các biện pháp can thiệp khác nhau và các mức độ tinh vi về phương pháp luận khác nhau. Kết quả của các nghiên cứu được xem xét này cung cấp cả sự lạc quan thận trọng về hiệu quả của các chương trình tại nơi làm việc này và những hiểu biết về các thành phần và đặc điểm thiết yếu của các chương trình thành công."} +{"idx": 1940058, "en": "In this article, we construct a ranking of occupations based upon the costs of job-related injuries and illnesses. Data are drawn from large nationally representative Bureau of Labor (BLS) data sets. Information is obtained on occupation and workers' compensation (WC) category of the injury or illness, which are then matched to information on costs. Six broad occupations and 413 specific (3-digit) occupations are ranked by total costs. Six broad and 223 specific occupations are ranked by costs per worker (average cost). Operators and laborers is the broad occupation category that contributes both the highest total and average cost. Specific occupations that contributed the most to total costs include heavy truck drivers, non-construction laborers, machine operators (not specified), occupations not classified, janitors, nursing orderlies, construction laborers, assemblers, retail sales workers (not elsewhere specified), miscellaneous machine operators, and carpenters. Occupations high on the average cost list include not-specified mechanics, general and construction laborers, press apprentices, welders, stone cutters, and warehouse workers. Although the BLS data are limited, they can be used to provide a preliminary look at which occupations are contributing the most and the least to the overall economic costs of occupational injuries and illnesses.", "gemini": "Trong bài viết này, chúng tôi xây dựng bảng xếp hạng các ngành nghề dựa trên chi phí thương tích và bệnh tật liên quan đến công việc. Dữ liệu được lấy từ các tập dữ liệu đại diện trên toàn quốc của Cục Lao động (BLS). Thông tin được thu thập về nghề nghiệp và danh mục bồi thường của công nhân (WC) về thương tích hoặc bệnh tật, sau đó được đối chiếu với thông tin về chi phí. Sáu ngành nghề rộng và 413 ngành nghề cụ thể (3 chữ số) được xếp hạng theo tổng chi phí. Sáu ngành nghề rộng và 223 ngành nghề cụ thể được xếp hạng theo chi phí cho mỗi công nhân (chi phí trung bình). Người vận hành và công nhân là nhóm nghề rộng đóng góp cả tổng chi phí và chi phí trung bình cao nhất. Các ngành nghề cụ thể đóng góp nhiều nhất vào tổng chi phí bao gồm tài xế xe tải hạng nặng, công nhân không làm việc trong ngành xây dựng, người vận hành máy móc (không xác định), nghề nghiệp không được phân loại, người gác cổng, người giúp việc điều dưỡng, công nhân xây dựng, thợ lắp ráp, nhân viên bán lẻ (không được chỉ định ở nơi khác), người vận hành máy móc khác và thợ mộc. Các ngành nghề có mức chi phí trung bình cao bao gồm thợ cơ khí không xác định, công nhân xây dựng và công nhân nói chung, học viên ép, thợ hàn, thợ cắt đá và công nhân kho. Mặc dù dữ liệu của BLS có hạn, nhưng chúng có thể được sử dụng để cung cấp cái nhìn sơ bộ về ngành nghề nào đóng góp nhiều nhất và ít nhất vào tổng chi phí kinh tế của thương tích và bệnh tật nghề nghiệp."} +{"idx": 1940059, "en": "The occupational and environmental medicine (OEM) gap in US medical education is widely recognized. In 1992, a federal initiative stimulated a primary care approach to improve residency training in South Carolina. This three-part report documents progress in designing and implementing an OEM curriculum, which is family medicine-centered. Each of the state's residency training programs participate in an ongoing Environmental Medicine Curriculum Committee effort. Part 1, discusses the needs assessment; Part 2, the five key elements of curriculum; and Part 3 details a clinical guide to the OEM patient.", "gemini": "Khoảng cách về y học nghề nghiệp và môi trường (OEM) trong giáo dục y khoa Hoa Kỳ được công nhận rộng rãi. Năm 1992, một sáng kiến của liên bang đã thúc đẩy một cách tiếp cận chăm sóc ban đầu để cải thiện đào tạo nội trú tại Nam Carolina. Báo cáo gồm ba phần này ghi lại tiến trình thiết kế và triển khai chương trình giảng dạy OEM, tập trung vào y học gia đình. Mỗi chương trình đào tạo nội trú của tiểu bang đều tham gia vào nỗ lực liên tục của Ủy ban Chương trình giảng dạy Y học Môi trường. Phần 1 thảo luận về đánh giá nhu cầu; Phần 2, năm yếu tố chính của chương trình giảng dạy; và Phần 3 trình bày chi tiết hướng dẫn lâm sàng cho bệnh nhân OEM."} +{"idx": 1940060, "en": "Part II of our three-part report examines five of the key elements for occupational and environmental medicine (OEM) training in family medicine residency. These were introduced by the Environmental Medicine Curriculum Committee (EMCC) faculty in South Carolina under a Department of Energy (DOE) grant to the Environmental Hazards Assessment Program (EHAP) of the Medical University of South Carolina, 1992-1997. Each element is being designed, tested, and updated by clinicians in the residency network. A pilot study of 100 third-year medical students conducted in 1996 suggests the difficulty in implementing OEM skills in current family medicine training.", "gemini": "Phần II trong báo cáo gồm ba phần của chúng tôi sẽ xem xét năm yếu tố chính để đào tạo y học nghề nghiệp và môi trường (OEM) trong chương trình nội trú y gia đình. Những yếu tố này đã được khoa Ủy ban chương trình giảng dạy y học môi trường (EMCC) ở Nam Carolina giới thiệu theo khoản tài trợ của Bộ năng lượng (DOE) cho Chương trình đánh giá mối nguy hiểm môi trường (EHAP) của Đại học Y khoa Nam Carolina, 1992-1997. Mỗi yếu tố đang được các bác sĩ lâm sàng trong mạng lưới nội trú thiết kế, thử nghiệm và cập nhật. Một nghiên cứu thí điểm trên 100 sinh viên y khoa năm thứ ba được tiến hành vào năm 1996 cho thấy khó khăn trong việc triển khai các kỹ năng OEM trong chương trình đào tạo y gia đình hiện tại."} +{"idx": 1940061, "en": "A four-prototype approach to the occupational and environmental medicine (OEM) patient in a busy primary care setting is described. A 2 x 2 table illustrates the two diagnostic, interrelated tasks during the outpatient, non-urgent visit: (a) sick? yes/no, and (b) exposed? yes/no. One may have the basic skills for task (a) but feel insecure for task (b). With OEM experience, creative use of resources (databases and consultants), and patient cooperation, a better balance between task (a) and task (b) can be achieved. The Environmental Medicine Curriculum Committee (EMCC) initiative described in Part I and Part II of this study has developed this patient-centered model to help the resident in training cope as he or she tries to deal with emerging patient concerns from the workplace and beyond. In November 1996, an expert panel of consultants representing OEM, public health, and family medicine endorsed the prototype OEM patient model for further development.", "gemini": "Một phương pháp tiếp cận bốn nguyên mẫu đối với bệnh nhân y học nghề nghiệp và môi trường (OEM) trong bối cảnh chăm sóc ban đầu bận rộn được mô tả. Một bảng 2 x 2 minh họa hai nhiệm vụ chẩn đoán có liên quan với nhau trong quá trình khám ngoại trú, không cấp cứu: (a) ốm? có/không và (b) có tiếp xúc? có/không. Người ta có thể có các kỹ năng cơ bản cho nhiệm vụ (a) nhưng cảm thấy không an toàn cho nhiệm vụ (b). Với kinh nghiệm OEM, việc sử dụng sáng tạo các nguồn lực (cơ sở dữ liệu và chuyên gia tư vấn) và sự hợp tác của bệnh nhân, có thể đạt được sự cân bằng tốt hơn giữa nhiệm vụ (a) và nhiệm vụ (b). Sáng kiến của Ủy ban chương trình giảng dạy y học môi trường (EMCC) được mô tả trong Phần I và Phần II của nghiên cứu này đã phát triển mô hình lấy bệnh nhân làm trung tâm này để giúp các bác sĩ nội trú trong quá trình đào tạo đối phó khi họ cố gắng giải quyết các mối quan tâm mới nổi của bệnh nhân từ nơi làm việc và hơn thế nữa. Vào tháng 11 năm 1996, một nhóm chuyên gia tư vấn đại diện cho OEM, y tế công cộng và y gia đình đã xác nhận mô hình bệnh nhân OEM nguyên mẫu để phát triển thêm."} +{"idx": 1940062, "en": "Questionnaire-based measures of function have been validated extensively in studies of chronic illness and work-related low back pain. These measures have only recently been developed for upper extremity disorders (UEDs), and there is little information on their utility in evaluation of injured workers. We developed the Upper Extremity Function Scale (UEFS), an eight-item, self-administered questionnaire, to measure the impacts of UEDs on function. This instrument was tested in a cohort of 108 patients with work-related UEDs and 165 patients with the carpal tunnel syndrome (CTS); both groups were enrolled in prospective follow-up studies. The UEFS demonstrated excellent psychometric properties, including good internal consistency (Cronbach's alpha > 0.83), relative absence of floor effects, and excellent convergent and discriminant validity, compared with measures of symptom severity and clinical findings. In the CTS group, the UEFS was more responsive to significant improvements over time than clinical measures such as grip and pinch strength. These data support the use of a self-reported functional scale as a measure of outcome in studies of work-related UEDs. Further investigations in working populations are needed to substantiate its utility in workers with UEDs who have not yet sought medical care.", "gemini": "Các biện pháp đánh giá chức năng dựa trên bảng câu hỏi đã được xác nhận rộng rãi trong các nghiên cứu về bệnh mãn tính và đau lưng dưới liên quan đến công việc. Các biện pháp này mới chỉ được phát triển gần đây cho các rối loạn chi trên (UED) và có rất ít thông tin về tiện ích của chúng trong việc đánh giá những người lao động bị thương. Chúng tôi đã phát triển Thang đánh giá chức năng chi trên (UEFS), một bảng câu hỏi tự quản lý gồm tám mục, để đo lường tác động của UED đối với chức năng. Công cụ này đã được thử nghiệm trên một nhóm 108 bệnh nhân mắc UED liên quan đến công việc và 165 bệnh nhân mắc hội chứng ống cổ tay (CTS); cả hai nhóm đều được ghi danh vào các nghiên cứu theo dõi triển vọng. UEFS đã chứng minh các đặc tính trắc nghiệm tâm lý tuyệt vời, bao gồm độ tin cậy nội bộ tốt (alpha Cronbach > 0,83), không có hiệu ứng sàn tương đối và độ tin cậy phân biệt và hội tụ tuyệt vời, so với các biện pháp về mức độ nghiêm trọng của triệu chứng và các phát hiện lâm sàng. Trong nhóm CTS, UEFS phản ứng tốt hơn với những cải thiện đáng kể theo thời gian so với các biện pháp lâm sàng như sức cầm và sức bóp. Những dữ liệu này hỗ trợ việc sử dụng thang đánh giá chức năng tự báo cáo như một biện pháp đánh giá kết quả trong các nghiên cứu về UED liên quan đến công việc. Cần có thêm các cuộc điều tra ở những người đang làm việc để chứng minh tính hữu ích của nó đối với những người lao động mắc UED nhưng chưa được chăm sóc y tế."} +{"idx": 1940063, "en": "Subject matter experts from 13 North American corporations provided detailed descriptions of the historical development and the current components and operations of their office ergonomics programs. Results were summarized across corporations and presented for the following programmatic topics: backgrounds of key people, initial awareness and preliminary needs assessment, program development, program implementation, program monitoring and evaluation, program components, education and training, workstation and job analysis, early identification of cases, case management, and alternate office environments. The subject matter experts also provided comments about the strengths of their programs, their advice to others, and lessons they learned. These observations suggested the need for an office ergonomics program, and possibly other occupational health programs, to fit into a corporation's culture and capitalize on its infrastructure. Most corporations used multidisciplinary task forces or teams to develop their programs. Communication, which included training, awareness, advertising, and feedback, was also an important issue. Flexibility and simplicity were important attributes of these programs. It is hoped that this descriptive information will be helpful to some occupational health managers interested in or concerned about managerial perspectives and skills related to the development and implementation of programs within their own corporations.", "gemini": "Các chuyên gia về vấn đề này từ 13 tập đoàn Bắc Mỹ đã cung cấp mô tả chi tiết về sự phát triển lịch sử cũng như các thành phần và hoạt động hiện tại của các chương trình công thái học văn phòng của họ. Kết quả được tóm tắt trên các tập đoàn và được trình bày cho các chủ đề lập trình sau: lý lịch của những người chủ chốt, nhận thức ban đầu và đánh giá nhu cầu sơ bộ, phát triển chương trình, triển khai chương trình, giám sát và đánh giá chương trình, các thành phần của chương trình, giáo dục và đào tạo, phân tích việc làm và trạm làm việc, xác định sớm các trường hợp, quản lý trường hợp và môi trường văn phòng thay thế. Các chuyên gia về vấn đề này cũng đưa ra các bình luận về điểm mạnh của các chương trình của họ, lời khuyên của họ cho người khác và những bài học mà họ đã học được. Những quan sát này cho thấy nhu cầu về một chương trình công thái học văn phòng, và có thể là các chương trình sức khỏe nghề nghiệp khác, phù hợp với văn hóa của một công ty và tận dụng cơ sở hạ tầng của công ty đó. Hầu hết các công ty đều sử dụng các nhóm hoặc lực lượng đặc nhiệm đa ngành để phát triển các chương trình của họ. Giao tiếp, bao gồm đào tạo, nhận thức, quảng cáo và phản hồi, cũng là một vấn đề quan trọng. Tính linh hoạt và đơn giản là những thuộc tính quan trọng của các chương trình này. Hy vọng rằng thông tin mô tả này sẽ hữu ích cho một số người quản lý sức khỏe nghề nghiệp quan tâm hoặc lo lắng về các kỹ năng và quan điểm quản lý liên quan đến việc phát triển và triển khai các chương trình trong các công ty của họ."} +{"idx": 1940064, "en": "The US Public Health Service (PHS) published new recommendations for preventing HIV seroconversion in health care workers suffering needle-stick injuries. In response, health care institutions across the United States are updating their internal protocols to meet the currently accepted standard of care in this area. We found the new guidelines lacking in critical definitions necessary to clarify practical applications in specific cases. We also found the recommendations of when to use one, two, or three drugs for post-exposure prophylaxis confusing. We report our university hospital's experience with needle-stick injuries over the past year. We propose modified guidelines that more finely stratify risk categories, define clinical variables which occur in practice, and simplify the algorithm for case management. The cost of managing our cases according to the modified guidelines are compared with management costs using the PHS guidelines. Potential costs of applying the PHS guidelines in our institution ranged from $68,994-$260,544; according to our modified guidelines, costs would range from $19,199-$54,749. We also present an analysis of the number of injured health care workers who need to be treated to prevent one case of seroconversion in the various risk stratifications.", "gemini": "Dịch vụ Y tế Công cộng Hoa Kỳ (PHS) đã công bố các khuyến nghị mới để ngăn ngừa nhiễm HIV ở những nhân viên y tế bị thương do kim tiêm. Để đáp lại, các cơ sở chăm sóc sức khỏe trên khắp Hoa Kỳ đang cập nhật các giao thức nội bộ của họ để đáp ứng tiêu chuẩn chăm sóc hiện được chấp nhận trong lĩnh vực này. Chúng tôi thấy các hướng dẫn mới còn thiếu các định nghĩa quan trọng cần thiết để làm rõ các ứng dụng thực tế trong các trường hợp cụ thể. Chúng tôi cũng thấy các khuyến nghị về thời điểm sử dụng một, hai hoặc ba loại thuốc để dự phòng sau phơi nhiễm rất khó hiểu. Chúng tôi báo cáo kinh nghiệm của bệnh viện trường đại học của chúng tôi về các trường hợp thương tích do kim tiêm trong năm qua. Chúng tôi đề xuất các hướng dẫn đã sửa đổi phân tầng các loại rủi ro một cách chi tiết hơn, xác định các biến số lâm sàng xảy ra trong thực tế và đơn giản hóa thuật toán để quản lý trường hợp. Chi phí quản lý các trường hợp của chúng tôi theo các hướng dẫn đã sửa đổi được so sánh với chi phí quản lý theo các hướng dẫn của PHS. Chi phí tiềm ẩn khi áp dụng các hướng dẫn của PHS tại cơ sở của chúng tôi dao động từ 68.994 đến 260.544 đô la; theo các hướng dẫn đã sửa đổi của chúng tôi, chi phí sẽ dao động từ 19.199 đến 54.749 đô la. Chúng tôi cũng trình bày một phân tích về số lượng nhân viên y tế bị thương cần được điều trị để ngăn ngừa một trường hợp nhiễm trùng ở các phân tầng rủi ro khác nhau."} +{"idx": 1940065, "en": "The purpose of this study was to establish a simple observation to help in distinguishing patients who are giving their maximal effort during computer-assisted grip strength measurements from those who are not. Preliminary observations had suggested that maximal effort with this grip-strength device was characterized by greater force in the radial digits than the ulnar digits and a small variability in force between individual grasps. These observations were then prospectively analyzed in the following groups: (a) 178 assembly workers tested during a routine screening at a computer manufacturing company and during pre-placement physicals; (b) 196 workers with a hand injury or cumulative trauma disorder who were seeking consultation for medical treatment; (c) 55 workers with a hand injury or cumulative trauma disorder being measured to determine their return to work status or impairment rating; and (d) 58 asymptomatic volunteers. The volunteers were instructed to perform the maximal grip in the same fashion as the other groups and then to repeat the test exerting a submaximal effort they estimated to be 50% of their own maximal effort. The results of this study demonstrated that there is a highly significant association, P < 0.001, between the presence of a \"total pattern\" (radial over ulnar force being less than 1.0, unilateral grip variation greater than 15%, and difference between the left and right grip variation greater than 5%) and the presence of submaximal effort during grip-strength measurements.", "gemini": "Mục đích của nghiên cứu này là thiết lập một quan sát đơn giản để giúp phân biệt những bệnh nhân đang cố gắng hết sức trong quá trình đo sức cầm nắm bằng máy tính với những bệnh nhân không cố gắng hết sức. Các quan sát sơ bộ cho thấy rằng nỗ lực tối đa với thiết bị đo sức cầm nắm này được đặc trưng bởi lực lớn hơn ở các ngón tay hướng ra ngoài so với các ngón tay hướng vào trong và sự thay đổi lực nhỏ giữa các lần cầm nắm riêng lẻ. Sau đó, các quan sát này đã được phân tích theo hướng tương lai trong các nhóm sau: (a) 178 công nhân lắp ráp được kiểm tra trong quá trình sàng lọc thường quy tại một công ty sản xuất máy tính và trong quá trình kiểm tra sức khỏe trước khi tuyển dụng; (b) 196 công nhân bị thương ở tay hoặc rối loạn chấn thương tích lũy đang tìm kiếm tư vấn để điều trị y tế; (c) 55 công nhân bị thương ở tay hoặc rối loạn chấn thương tích lũy được đo để xác định tình trạng trở lại làm việc hoặc mức độ suy yếu; và (d) 58 tình nguyện viên không có triệu chứng. Những người tình nguyện được hướng dẫn thực hiện việc cầm nắm tối đa theo cách giống như các nhóm khác và sau đó lặp lại bài kiểm tra bằng cách dùng một lực nhỏ hơn mà họ ước tính là 50% so với lực tối đa của chính họ. Kết quả của nghiên cứu này chứng minh rằng có mối liên hệ rất có ý nghĩa, P < 0,001, giữa sự hiện diện của một \"mô hình tổng thể\" (lực hướng ra ngoài trên lực hướng vào trong nhỏ hơn 1,0, sự thay đổi lực cầm nắm một bên lớn hơn 15% và sự khác biệt giữa sự thay đổi lực cầm nắm bên trái và bên phải lớn hơn 5%) và sự hiện diện của nỗ lực nhỏ hơn tối đa trong quá trình đo sức cầm nắm."} +{"idx": 1940066, "en": "An historical follow-up study of 528 model makers in a German automobile company found an unusual pattern of mortality among these workers that included an increased risk of total cancer and tumors of the stomach, genitourinary organs, and the brain (Becker et al, 1992). Because of small numbers of cases and problems with the validity of causes of death, we carried out a second follow-up using data from the official mortality statistics and extending the observation period by five years. The new results did not confirm the previous observations of an increased risk of total cancer, cancer of the genitourinary organs, and tumors of the brain but confirmed indications of an increased risk of stomach cancer and suggested for the first time that there may be an increased risk of cancer of the intestine. Though the small numbers of cases did not allow statistical confirmation of these findings, they are consistent with the results of other studies recently conducted in the United States and published in this journal.", "gemini": "Một nghiên cứu theo dõi theo thời gian có tính lịch sử về 528 người làm mô hình trong một công ty ô tô của Đức đã phát hiện ra một mô hình tử vong bất thường ở những công nhân này bao gồm nguy cơ ung thư nói chung và khối u ở dạ dày, cơ quan sinh dục tiết niệu và não tăng (Becker et al, 1992). Do số lượng ca ít và có vấn đề về tính hợp lệ của nguyên nhân tử vong, chúng tôi đã tiến hành theo dõi lần thứ hai bằng cách sử dụng dữ liệu từ số liệu thống kê tử vong chính thức và kéo dài thời gian quan sát thêm năm năm. Các kết quả mới không xác nhận những quan sát trước đó về nguy cơ ung thư nói chung, ung thư cơ quan sinh dục tiết niệu và khối u não tăng nhưng đã xác nhận các dấu hiệu về nguy cơ ung thư dạ dày tăng và lần đầu tiên đưa ra gợi ý rằng có thể có nguy cơ ung thư ruột tăng. Mặc dù số lượng ca ít không cho phép xác nhận thống kê những phát hiện này, nhưng chúng phù hợp với kết quả của các nghiên cứu khác gần đây được thực hiện ở Hoa Kỳ và được công bố trên tạp chí này."} +{"idx": 1940067, "en": "Washington's late night retail worker crime protection regulation, enforced by the state Occupational Safety and Health Administration (OSHA) program, was intended to prevent injuries by deterring violent crimes. We investigated whether the regulation was associated with businesses' violence prevention activities. We surveyed 1,516 employers at high risk of robbery, including gas stations, groceries, hotels, restaurants, and taverns, in 1995 to determine whether they had violence prevention training programs for their employees (a requirement of the standard). Awareness of the regulation was low (4.4%). Employers covered by the regulation were more likely to have programs (Odds Ratio [OR] = 1.4), as were those aware of a regulation (OR = 3.4). State OSHA plan contact (an inspection or consultation) was also associated with having a program (OR = 1.9). Despite low awareness of the standard, results suggested that regulatory efforts to protect high-risk employees were associated with employers' robbery and crime prevention activities.", "gemini": "Quy định bảo vệ tội phạm của nhân viên bán lẻ đêm muộn của Washington, được thực thi bởi chương trình Quản lý An toàn và Sức khỏe Nghề nghiệp của tiểu bang (OSHA), nhằm mục đích ngăn ngừa thương tích bằng cách ngăn chặn các tội phạm bạo lực. Chúng tôi đã điều tra xem quy định này có liên quan đến các hoạt động phòng chống bạo lực của doanh nghiệp hay không. Chúng tôi đã khảo sát 1.516 chủ lao động có nguy cơ bị cướp cao, bao gồm trạm xăng, cửa hàng tạp hóa, khách sạn, nhà hàng và quán rượu, vào năm 1995 để xác định xem họ có chương trình đào tạo phòng chống bạo lực cho nhân viên của mình hay không (một yêu cầu của tiêu chuẩn). Nhận thức về quy định này còn thấp (4,4%). Những người sử dụng lao động được bao phủ bởi quy định này có nhiều khả năng có các chương trình (Tỷ lệ cược [OR] = 1,4), cũng như những người nhận thức được một quy định (OR = 3,4). Liên hệ với kế hoạch OSHA của tiểu bang (kiểm tra hoặc tư vấn) cũng có liên quan đến việc có một chương trình (OR = 1,9). Mặc dù nhận thức về tiêu chuẩn còn thấp, nhưng kết quả cho thấy các nỗ lực quản lý để bảo vệ những nhân viên có nguy cơ cao có liên quan đến hoạt động phòng chống cướp và tội phạm của người sử dụng lao động."} +{"idx": 1940068, "en": "Artificial nutritional support may be supplied by several routes. Its many potential complications can be avoided if they are anticipated and help sought early from the nutrition team.", "gemini": "Có thể cung cấp hỗ trợ dinh dưỡng nhân tạo theo nhiều cách. Có thể tránh được nhiều biến chứng tiềm ẩn của nó nếu chúng được dự đoán trước và tìm kiếm sự trợ giúp sớm từ nhóm dinh dưỡng."} +{"idx": 1940069, "en": "This paper defines who needs a community-based rehabilitation service and the specific requirements of the four different client groups. A model is proposed whereby such a service might be delivered to those groups. It is possible that other specialties, for example rheumatology, might wish to develop similar models of care.", "gemini": "Bài báo này xác định những người cần dịch vụ phục hồi chức năng dựa vào cộng đồng và các yêu cầu cụ thể của bốn nhóm khách hàng khác nhau. Một mô hình được đề xuất theo đó dịch vụ như vậy có thể được cung cấp cho những nhóm đó. Có thể các chuyên khoa khác, chẳng hạn như thấp khớp học, có thể muốn phát triển các mô hình chăm sóc tương tự."} +{"idx": 1940070, "en": "A pathologist appointed by the coroner may feel that his or her role is to review the medical notes, perform a post-mortem, examination and then interpret the findings in the light of clinical information and any other information received from the coroner, and include in the clinico-pathological summary a cause of death. We believe that such an approach is not in accordance with the legal position relating to coroners' inquests. The coroner has no automatic right to see the medical notes (and neither does the coroner's pathologist); where there is, or may be, dispute as to the circumstances leading to death, the proper way for information in the medical record to be presented at the coroner's inquest is for the maker of any note to give oral evidence. Where the cause of death requires interpretation of the clinical history or knowledge of any circumstantial evidence, a pathologist should refrain from giving a cause of death; such a task is for the court, having heard all the evidence-medical or not-relating to the death.", "gemini": "Một bác sĩ giải phẫu bệnh được chỉ định bởi cảnh sát điều tra có thể cảm thấy rằng vai trò của mình là xem xét các ghi chú y tế, thực hiện khám nghiệm tử thi và sau đó diễn giải các phát hiện dựa trên thông tin lâm sàng và bất kỳ thông tin nào khác nhận được từ cảnh sát điều tra, và bao gồm trong tóm tắt lâm sàng-bệnh học nguyên nhân tử vong. Chúng tôi tin rằng cách tiếp cận như vậy không phù hợp với vị trí pháp lý liên quan đến cuộc điều tra của cảnh sát điều tra. Cảnh sát điều tra không có quyền tự động xem các ghi chú y tế (và bác sĩ giải phẫu bệnh của cảnh sát điều tra cũng không); trong trường hợp có hoặc có thể có tranh chấp về các tình tiết dẫn đến tử vong, cách thích hợp để trình bày thông tin trong hồ sơ y tế tại cuộc điều tra của cảnh sát điều tra là người lập bất kỳ ghi chú nào đều phải đưa ra bằng chứng bằng miệng. Trong trường hợp nguyên nhân tử vong đòi hỏi phải giải thích tiền sử lâm sàng hoặc hiểu biết về bất kỳ bằng chứng tình huống nào, bác sĩ giải phẫu bệnh nên tránh đưa ra nguyên nhân tử vong; một nhiệm vụ như vậy là dành cho tòa án, sau khi nghe tất cả các bằng chứng - y tế hoặc không liên quan đến cái chết."} +{"idx": 1940071, "en": "OBJECTIVE To improve the quality of the percutaneous tunnelled central venous catheter placement service for patients being treated for malignant disease. DESIGN A clinical nurse specialist was specially trained to insert percutaneous tunnelled central venous catheters according to predetermined guidelines. Catheters were inserted under local anaesthetic in the outpatient department or the ward. The quality of the service was analysed and compared with the pre-existing service provided by junior medical staff. SUBJECTS Two hundred adult patients with malignant disease seen between January 1995 and January 1996 at the Christie Hospital Trust. MAIN OUTCOME MEASURES Success of the procedure, insertion-related infection rates and waiting times compared to historical controls. RESULTS The rate of failed insertions fell from 20% to 3% with a concomitant reduction in surgical referrals; for 97% of patients waiting time was reduced to less than one working day compared with 80% previously. Line-related infection rates in the first thirty days following insertion fell from 10 episodes per 72 lines inserted to two episodes per 200 lines inserted. CONCLUSIONS Training and using a clinical nurse specialist has improved the quality of service and gives junior doctors more opportunity to become competent in the technique of central venous catheter placement. The introduction of guidelines has encouraged a standard approach that facilitates audit.", "gemini": "MỤC TIÊU Cải thiện chất lượng dịch vụ đặt ống thông tĩnh mạch trung tâm qua da có đường hầm cho bệnh nhân đang được điều trị bệnh ác tính. THIẾT KẾ Một y tá lâm sàng chuyên khoa đã được đào tạo đặc biệt để đặt ống thông tĩnh mạch trung tâm qua da có đường hầm theo các hướng dẫn được định trước. Ống thông được đặt dưới gây tê tại chỗ ở khoa ngoại trú hoặc khoa. Chất lượng dịch vụ được phân tích và so sánh với dịch vụ hiện có do nhân viên y tế cơ sở cung cấp. ĐỐI TƯỢNG Hai trăm bệnh nhân trưởng thành mắc bệnh ác tính được khám từ tháng 1 năm 1995 đến tháng 1 năm 1996 tại Christie Hospital Trust. CÁC BIỆN PHÁP ĐO KẾT QUẢ CHÍNH Tỷ lệ thành công của thủ thuật, tỷ lệ nhiễm trùng liên quan đến việc đặt và thời gian chờ so với các đối chứng trong quá khứ. KẾT QUẢ Tỷ lệ đặt thất bại giảm từ 20% xuống 3% cùng với việc giảm số lần chuyển đến khoa phẫu thuật; đối với 97% bệnh nhân, thời gian chờ đã giảm xuống dưới một ngày làm việc so với trước đây là 80%. Tỷ lệ nhiễm trùng liên quan đến đường truyền trong ba mươi ngày đầu sau khi đặt giảm từ 10 đợt trên 72 đường truyền được đặt xuống còn hai đợt trên 200 đường truyền được đặt. KẾT LUẬN Việc đào tạo và sử dụng một y tá lâm sàng chuyên khoa đã cải thiện chất lượng dịch vụ và mang lại cho các bác sĩ cơ hội tốt hơn để trở nên thành thạo trong kỹ thuật đặt ống thông tĩnh mạch trung tâm. Việc đưa ra các hướng dẫn đã khuyến khích một cách tiếp cận chuẩn giúp tạo điều kiện cho việc kiểm toán."} +{"idx": 1940072, "en": "OBJECTIVE To assess the skills and opinions of different grades of doctors and cardiac-trained nurses in interpreting electrocardiographic changes when deciding upon administration of thrombolysis to patients with chest pain. DESIGN A questionnaire was distributed to staff in several local hospitals. SUBJECTS AND METHODS Participants were asked to assess 30 electrocardiograms (ECGs) and determine whether they would prescribe thrombolytic therapy on the basis of each one, assuming an associated typical history of acute myocardial infarction (AMI) and no contra-indications to treatment. They were asked to return the questionnaire anonymously, stating only their position. RESULTS Of the 88 questionnaires, 61 were returned by 15 senior nurses, 10 house officers, 12 senior house officers, 10 medical registrars, eight consultant physicians and six consultant cardiologists. When electrocardiograms showed unequivocal evidence of acute myocardial infarction, all consultant cardiologists gave the correct answer, but only 75.5% of house officers diagnosed AMI. Cardiologists were most in favour of thrombolysis when left bundle branch block was present. Cardiac-trained nurses showed good decision-making skills. CONCLUSIONS Staff involved in assessment of patients with chest pain should have specific training in electrocardiographic diagnosis of myocardial infarction in order to minimise in-hospital delay when thrombolysis is indicated. The management of patients with left bundle branch block remains uncertain; cardiologists are more likely to recommend thrombolytic therapy than any of the other participants in the study.", "gemini": "MỤC TIÊU Đánh giá các kỹ năng và ý kiến của các bác sĩ và điều dưỡng được đào tạo về tim mạch ở các cấp độ khác nhau trong việc giải thích các thay đổi trên điện tâm đồ khi quyết định dùng thuốc tiêu sợi huyết cho bệnh nhân đau ngực. THIẾT KẾ Một bảng câu hỏi đã được phân phối cho nhân viên tại một số bệnh viện địa phương. ĐỐI TƯỢNG VÀ PHƯƠNG PHÁP Những người tham gia được yêu cầu đánh giá 30 điện tâm đồ (ECG) và xác định xem họ có kê đơn liệu pháp tiêu sợi huyết dựa trên từng điện tâm đồ hay không, giả sử có tiền sử điển hình của nhồi máu cơ tim cấp (AMI) và không có chống chỉ định điều trị. Họ được yêu cầu trả lại bảng câu hỏi ẩn danh, chỉ nêu chức vụ của họ. KẾT QUẢ Trong số 88 bảng câu hỏi, 61 bảng được trả lại bởi 15 điều dưỡng cao cấp, 10 bác sĩ nội trú, 12 bác sĩ nội trú cao cấp, 10 bác sĩ nội trú, tám bác sĩ chuyên khoa và sáu bác sĩ tim mạch. Khi điện tâm đồ cho thấy bằng chứng rõ ràng về nhồi máu cơ tim cấp, tất cả các bác sĩ tim mạch đều đưa ra câu trả lời đúng, nhưng chỉ có 75,5% bác sĩ nội trú chẩn đoán AMI. Các bác sĩ tim mạch ủng hộ liệu pháp tiêu sợi huyết nhất khi có block nhánh trái. Điều dưỡng được đào tạo về tim mạch cho thấy kỹ năng ra quyết định tốt. KẾT LUẬN Các nhân viên tham gia đánh giá bệnh nhân đau ngực nên được đào tạo cụ thể về chẩn đoán nhồi máu cơ tim bằng điện tâm đồ để giảm thiểu sự chậm trễ trong bệnh viện khi cần chỉ định thuốc tiêu sợi huyết. Việc quản lý bệnh nhân bị block nhánh trái vẫn chưa chắc chắn; các bác sĩ tim mạch có nhiều khả năng đề nghị liệu pháp tiêu sợi huyết hơn bất kỳ người tham gia nào khác trong nghiên cứu."} +{"idx": 1940073, "en": "The relationship of raised serum cholesterol to coronary atherosclerosis, the main pathophysiological substrate for most ischaemic myocardial events, is clear. Strokes have a more complex aetiology. Hypertension and smoking are strong risk factors for stroke, and their elimination is effective in stroke prevention, but cholesterol has been regarded historically as a poor predictor of stroke incidence. The reasons for this are analysed and the relevance of serum cholesterol to stroke prevention discussed.", "gemini": "Mối quan hệ giữa cholesterol huyết thanh tăng cao với xơ vữa động mạch vành, là nền tảng bệnh sinh chính cho hầu hết các biến cố nhồi máu cơ tim do thiếu máu cục bộ, là rất rõ ràng. Đột quỵ có nguyên nhân phức tạp hơn. Tăng huyết áp và hút thuốc là những yếu tố nguy cơ cao gây đột quỵ và việc loại bỏ chúng có hiệu quả trong việc phòng ngừa đột quỵ, nhưng trong lịch sử, cholesterol được coi là yếu tố dự báo kém về tỷ lệ đột quỵ. Các lý do cho điều này được phân tích và thảo luận về mối liên quan của cholesterol huyết thanh với việc phòng ngừa đột quỵ."} +{"idx": 1940074, "en": "Medical research workers are making increasing use of logistic regression analysis for binary and ordinal data. The purpose of this paper is to give a non-technical introduction to logistic regression models for ordinal response variables. We address issues such as the global concept and interpretation of logistic models, the model building procedure from a practical point of view, and the assessment of the model adequacy. For illustrative purposes we apply these methods to real data of a study investigating the association between glycosylated haemoglobin and retinopathy. We give some recommendations for the use and assessment of ordinal logistic regression models in medical research.", "gemini": "Các nhà nghiên cứu y khoa đang ngày càng sử dụng nhiều hơn phân tích hồi quy logistic cho dữ liệu nhị phân và thứ tự. Mục đích của bài báo này là cung cấp một phần giới thiệu không mang tính kỹ thuật về các mô hình hồi quy logistic cho các biến phản ứng thứ tự. Chúng tôi giải quyết các vấn đề như khái niệm toàn cầu và cách giải thích các mô hình logistic, quy trình xây dựng mô hình theo quan điểm thực tế và đánh giá tính đầy đủ của mô hình. Để minh họa, chúng tôi áp dụng các phương pháp này vào dữ liệu thực tế của một nghiên cứu điều tra mối liên quan giữa hemoglobin glycosyl hóa và bệnh võng mạc. Chúng tôi đưa ra một số khuyến nghị về việc sử dụng và đánh giá các mô hình hồi quy logistic thứ tự trong nghiên cứu y khoa."} +{"idx": 1940075, "en": "It is becoming increasingly clear that the control of self-reactivity involves peripheral mechanisms that supplement thymic negative selection. It is now generally accepted that T-cell activation depends upon both T-cell receptor engagement and the delivery of B7-mediated costimulation by specialized antigen presenting cells (APC). In contrast, failure to deliver B7-mediated costimulation can result in the induction of antigen-specific non-responsiveness. In physiological terms, costimulation-deficient antigen presentation is the prerogative of those cells that do not express B7 molecules, even during inflammatory conditions, such as tissue parenchymal cells. The consequences of such costimulation-deficient antigen presentation are illustrated by the allospecific tolerance that is observed in animal models of transplantation following the depletion of bone marrow-derived APC from an allograft. In this paper the possible role of antigen presentation by tissue parenchymal cells in the induction and maintenance of peripheral tolerance is discussed, with particular attention to the important contribution that the liver may make to these events.", "gemini": "Ngày càng rõ ràng rằng sự kiểm soát phản ứng tự thân liên quan đến các cơ chế ngoại vi bổ sung cho sự chọn lọc âm tính của tuyến ức. Hiện nay, người ta chấp nhận rộng rãi rằng sự hoạt hóa tế bào T phụ thuộc vào cả sự gắn kết thụ thể tế bào T và sự cung cấp sự đồng kích thích do B7 trung gian bởi các tế bào chuyên biệt trình diện kháng nguyên (APC). Ngược lại, việc không cung cấp sự đồng kích thích do B7 trung gian có thể dẫn đến việc gây ra tình trạng không đáp ứng đặc hiệu với kháng nguyên. Về mặt sinh lý, sự trình diện kháng nguyên thiếu đồng kích thích là đặc quyền của những tế bào không biểu hiện các phân tử B7, ngay cả trong các tình trạng viêm, chẳng hạn như các tế bào nhu mô mô. Hậu quả của sự trình diện kháng nguyên thiếu đồng kích thích như vậy được minh họa bằng tình trạng dung nạp đặc hiệu với allo quan sát thấy ở các mô hình ghép tạng trên động vật sau khi loại bỏ APC có nguồn gốc từ tủy xương khỏi ghép dị loại. Trong bài báo này, vai trò có thể có của việc trình diện kháng nguyên bởi các tế bào nhu mô mô trong việc gây ra và duy trì tình trạng dung nạp ngoại vi được thảo luận, đặc biệt chú ý đến sự đóng góp quan trọng mà gan có thể thực hiện đối với các sự kiện này."} +{"idx": 1940076, "en": "BACKGROUND This is a study of an outpatient programme for rehabilitation of patients with severe ventilatory impairment due to chronic obstructive pulmonary disease (COPD). Its main purpose was to assess the feasibility of such a programme and so no control group was included. METHODS The study included 44 patients (28 men) of mean age 66 years with COPD. They all had severe ventilatory impairment as defined by a forced expiratory volume in 1 second (FEV1) less than 40% of predicted. Initial assessment included a shuttle walking test, the Chronic Respiratory Disease Questionnaire (CRDQ), the Hospital Anxiety and Depression scale (HAD) and the Sickness Impact Profile (SIP). The patients then entered a 6-week outpatient programme during which they attended twice weekly for a 2 1/2 hour session. Assessment was repeated on completion of the study (the 3 1/2 month assessment) and at 6 months. RESULTS The shuttle walking distance improved significantly and was maintained at the improved level for 6 months. The improvement in all four dimensions of the CRDQ was statistically significant and reached clinical significance for fatigue and for mastery. On entry, a notable level of depression was found in 32% of patients, and of anxiety in 40%. There was significant reduction in both of these which was maintained at 6 months. There was no improvement in the SIP at 3 months, but significant improvement was found at 6 months. CONCLUSIONS This study shows that a successful outpatient programme can be conducted in patients with severe ventilatory impairment, and that apparent benefit in physical ability and in health-related quality of life can be achieved. The improvements were maintained at 6 months.", "gemini": "BỐI CẢNH Đây là một nghiên cứu về chương trình phục hồi chức năng ngoại trú cho những bệnh nhân suy giảm chức năng thông khí nghiêm trọng do bệnh phổi tắc nghẽn mạn tính (COPD). Mục đích chính của nghiên cứu là đánh giá tính khả thi của một chương trình như vậy nên không có nhóm đối chứng nào được đưa vào. PHƯƠNG PHÁP Nghiên cứu bao gồm 44 bệnh nhân (28 nam) có độ tuổi trung bình là 66 tuổi mắc COPD. Tất cả đều bị suy giảm chức năng thông khí nghiêm trọng được xác định bằng thể tích thở ra gắng sức trong 1 giây (FEV1) nhỏ hơn 40% so với dự đoán. Đánh giá ban đầu bao gồm một bài kiểm tra đi bộ đưa đón, Bản câu hỏi về bệnh hô hấp mạn tính (CRDQ), thang đánh giá lo âu và trầm cảm của bệnh viện (HAD) và Hồ sơ tác động bệnh tật (SIP). Sau đó, bệnh nhân tham gia một chương trình ngoại trú kéo dài 6 tuần, trong đó họ đến hai lần một tuần cho một buổi kéo dài 2 giờ 1/2. Đánh giá được lặp lại sau khi hoàn thành nghiên cứu (đánh giá 3 tháng 1/2) và sau 6 tháng. KẾT QUẢ Khoảng cách đi bộ đưa đón được cải thiện đáng kể và được duy trì ở mức cải thiện trong 6 tháng. Sự cải thiện ở cả bốn chiều của CRDQ có ý nghĩa thống kê và đạt ý nghĩa lâm sàng đối với tình trạng mệt mỏi và khả năng kiểm soát. Khi nhập viện, 32% bệnh nhân được phát hiện có mức độ trầm cảm đáng kể và 40% có lo lắng. Có sự giảm đáng kể ở cả hai loại này và được duy trì trong 6 tháng. Không có sự cải thiện nào trong SIP ở tháng thứ 3, nhưng sự cải thiện đáng kể được phát hiện ở tháng thứ 6. KẾT LUẬN Nghiên cứu này cho thấy rằng một chương trình ngoại trú thành công có thể được tiến hành ở những bệnh nhân suy giảm chức năng thông khí nghiêm trọng và có thể đạt được lợi ích rõ ràng về khả năng thể chất và chất lượng cuộc sống liên quan đến sức khỏe. Những cải thiện này được duy trì trong 6 tháng."} +{"idx": 1940077, "en": "Cytokines are soluble mediators of intercellular communication. They contribute to a chemical signalling language that regulates development, tissue repair, haematopoiesis, inflammation and the immune response. Potent cytokine polypeptides have pleiotropic activities and functional redundancy. They act in a complex network where one cytokine can influence the production of, and response to, many other cytokines. In the past 5 years this bewildering array of 100+ effector molecules and associated cell surface receptors has been simplified by study of three-dimensional cytokine and cytokine receptor structure: elucidation of convergent intracellular signalling pathways; and molecular genetics, especially targeted gene disruption to 'knockout' production of individual cytokines in mice. It is also now clear that much of the pathophysiology of infectious disease can be explained by the induction of cytokines and the subsequent cellular response. Cytokine and cytokine antagonists have also shown therapeutic potential in a number of chronic and acute diseases.", "gemini": "Cytokine là chất trung gian hòa tan của quá trình giao tiếp giữa các tế bào. Chúng góp phần tạo nên một ngôn ngữ truyền tín hiệu hóa học điều chỉnh sự phát triển, sửa chữa mô, tạo máu, viêm và phản ứng miễn dịch. Các polypeptide cytokine mạnh có hoạt động đa hiệu và dự phòng chức năng. Chúng hoạt động trong một mạng lưới phức tạp, trong đó một cytokine có thể ảnh hưởng đến quá trình sản xuất và phản ứng với nhiều cytokine khác. Trong 5 năm qua, mảng các phân tử tác dụng và thụ thể bề mặt tế bào liên quan khổng lồ gồm hơn 100 phân tử này đã được đơn giản hóa bằng cách nghiên cứu cấu trúc cytokine và thụ thể cytokine ba chiều: làm sáng tỏ các con đường truyền tín hiệu nội bào hội tụ; và di truyền phân tử, đặc biệt là phá vỡ gen mục tiêu để 'vô hiệu hóa' quá trình sản xuất cytokine riêng lẻ ở chuột. Hiện nay cũng đã rõ ràng rằng phần lớn các bệnh sinh lý của bệnh truyền nhiễm có thể được giải thích bằng cách gây ra cytokine và phản ứng tế bào sau đó. Cytokine và chất đối kháng cytokine cũng đã cho thấy tiềm năng điều trị ở một số bệnh mãn tính và cấp tính."} +{"idx": 1940078, "en": "Using examples predominantly drawn from study of the lymphocytic choriomeningitis virus (LCMV) model system, this review describes the mechanisms involved in control of virus infections by the cell-mediated immune response, and some of the different strategies viruses have evolved to evade such immune clearance so that they can persist in their hosts. The important role played by the CD8+ cytotoxic lymphocyte (CTL) response in clearance of many systemic virus infections is discussed; and it is emphasized that although CD8+ CTL are classically thought of as lymphocytes which mediate lysis of virus-infected target cells, the principal mechanism by which CD8+ T cells effect clearance of persistent and many acute virus infections via production of antiviral cytokines such as tumour necrosis factor-alpha and interferon-gamma, not via destruction of virus-producing cells. To avoid immune-mediated clearance, viruses frequently use a combination of several different strategies. These can be grouped into mechanisms for avoiding recognition by the immune response (such as establishing latent infections, replicating in immune-privileged sites, down-regulating the expression of immune recognition signals on the surface of infected cells, or undergoing antigenic variation); and mechanisms for suppressing the immune response. The latter include generalized immune suppression mechanisms, and strategies for more precisely disabling the specific immune response such as inducing tolerance or exhaustion of virus-specific CTL. The value of understanding both immune clearance mechanisms and viral evasion strategies in the rational design of immune-based therapies to combat persistent virus infections is discussed.", "gemini": "Sử dụng các ví dụ chủ yếu lấy từ nghiên cứu về hệ thống mô hình virus viêm màng não lympho bào (LCMV), bài tổng quan này mô tả các cơ chế liên quan đến việc kiểm soát nhiễm trùng vi-rút bằng phản ứng miễn dịch do tế bào trung gian và một số chiến lược khác nhau mà vi-rút đã phát triển để trốn tránh sự thanh thải miễn dịch như vậy để chúng có thể tồn tại trong vật chủ của chúng. Vai trò quan trọng của phản ứng tế bào lympho độc tế bào CD8+ (CTL) trong việc thanh thải nhiều nhiễm trùng do vi-rút toàn thân được thảo luận; và nhấn mạnh rằng mặc dù các tế bào CTL CD8+ được coi là các tế bào lympho có chức năng trung gian phá hủy các tế bào đích bị nhiễm vi-rút, cơ chế chính mà các tế bào T CD8+ thực hiện để thanh thải các nhiễm trùng do vi-rút dai dẳng và nhiều loại vi-rút cấp tính là thông qua sản xuất các cytokine kháng vi-rút như yếu tố hoại tử khối u alpha và interferon gamma, không phải thông qua việc phá hủy các tế bào sản xuất vi-rút. Để tránh sự thanh thải do miễn dịch, vi-rút thường sử dụng kết hợp một số chiến lược khác nhau. Những chiến lược này có thể được nhóm thành các cơ chế để tránh sự nhận biết của phản ứng miễn dịch (như thiết lập các nhiễm trùng tiềm ẩn, sao chép tại các vị trí có đặc quyền miễn dịch, điều chỉnh giảm sự biểu hiện của các tín hiệu nhận dạng miễn dịch trên bề mặt của các tế bào bị nhiễm hoặc trải qua biến đổi kháng nguyên); và các cơ chế để ức chế phản ứng miễn dịch. Các cơ chế sau bao gồm các cơ chế ức chế miễn dịch toàn thể và các chiến lược để vô hiệu hóa chính xác hơn phản ứng miễn dịch đặc hiệu như gây ra tình trạng dung nạp hoặc kiệt sức của các tế bào CTL đặc hiệu với vi-rút. Giá trị của việc hiểu cả cơ chế thanh thải miễn dịch và các chiến lược trốn tránh vi-rút trong thiết kế hợp lý các liệu pháp dựa trên miễn dịch để chống lại các nhiễm trùng do vi-rút dai dẳng được thảo luận."} +{"idx": 1940079, "en": "Gregor Mendel is considered to be the founding father of modern genetics, and his laws of inheritance have led to the successful analysis of rare monogenic diseases such as cystic fibrosis, Duchenne muscular dystrophy, familial hypercholesterolaemia, and many others. Francis Galton chose multifactorial inheritance as his starting point, and his methods of analysis have withstood the test of time. He used detailed family records to study the inherited tendency of complex traits between parents and offspring, and between identical and non-identical twins to refine the analysis, and devised new statistics to attempt to measure the extent of inheritance. For all these reasons, he can be considered the founding father of quantitative genetics.", "gemini": "Gregor Mendel được coi là cha đẻ của di truyền học hiện đại và các định luật di truyền của ông đã dẫn đến việc phân tích thành công các bệnh đơn gen hiếm như xơ nang, loạn dưỡng cơ Duchenne, tăng cholesterol máu gia đình và nhiều bệnh khác. Francis Galton đã chọn di truyền đa nhân tố làm điểm khởi đầu của mình và các phương pháp phân tích của ông đã vượt qua thử thách của thời gian. Ông đã sử dụng hồ sơ gia đình chi tiết để nghiên cứu xu hướng di truyền các đặc điểm phức tạp giữa cha mẹ và con cái, và giữa cặp song sinh cùng trứng và không cùng trứng để tinh chỉnh phân tích và đưa ra các số liệu thống kê mới để cố gắng đo lường mức độ di truyền. Vì tất cả những lý do này, ông có thể được coi là cha đẻ của di truyền học định lượng."} +{"idx": 1940080, "en": "Hepatitis C virus (HCV) is notable for the high rate of chronic infection, which occurs in nearly all individuals who become infected. Liver biopsies from individuals with chronic HCV infection are notable for the presence of numerous mononuclear cells, at least some of which are CD4+ and CD8+ T lymphocytes. The immune response to HCV is polyclonal and multispecific, both in terms of antibody and cellular immune responses. Individuals who recover from acute HCV infection appear to have quantitatively more vigorous CD4+ proliferative responses against one or more HCV proteins compared with those individuals who develop chronic disease. CD8+ responses are less well characterized, in part because of the technical difficulties involved in isolating and characterizing these cells. HCV-specific CTL can be readily isolated from the liver and PBMC of chronically infected individuals, and recognize multiple epitopes. Even individuals with the same HLA type do not consistently recognize the same epitope. Thus, there does not appear to be an immunodominant response on the CD8+ level in this infection. CD8+ cells do appear to play some role in limiting viral replication. These responses are insufficient to eradicate virus completely, however, and may cause liver injury once chronic infection is established. Cytokines produced by both CD4+ and CD8+ cells may play an important role in both inhibiting viral replication and causing liver injury. A better understanding of the role of cellular immunity in the pathogenesis of HCV infection may aid in the development of vaccines and immunotherapeutic intervention strategies.", "gemini": "Virus viêm gan C (HCV) đáng chú ý vì tỷ lệ nhiễm trùng mãn tính cao, xảy ra ở hầu hết những người bị nhiễm. Sinh thiết gan từ những người bị nhiễm HCV mãn tính có đặc điểm là sự hiện diện của nhiều tế bào đơn nhân, trong đó ít nhất một số tế bào là tế bào lympho T CD4+ và CD8+. Phản ứng miễn dịch với HCV là đa dòng và đa đặc hiệu, cả về mặt kháng thể và phản ứng miễn dịch tế bào. Những người phục hồi sau nhiễm HCV cấp tính có vẻ như có phản ứng tăng sinh CD4+ mạnh mẽ hơn về mặt định lượng đối với một hoặc nhiều protein HCV so với những người mắc bệnh mãn tính. Phản ứng CD8+ ít được đặc trưng rõ hơn, một phần là do những khó khăn về mặt kỹ thuật liên quan đến việc phân lập và đặc trưng hóa các tế bào này. Các tế bào CTL đặc hiệu với HCV có thể dễ dàng phân lập từ gan và PBMC của những người bị nhiễm mãn tính và nhận ra nhiều epitop. Ngay cả những cá thể có cùng kiểu HLA cũng không nhất quán nhận ra cùng một epitop. Do đó, có vẻ như không có phản ứng miễn dịch trội ở mức CD8+ trong nhiễm trùng này. Các tế bào CD8+ dường như đóng một số vai trò trong việc hạn chế sự sao chép của vi-rút. Tuy nhiên, những phản ứng này không đủ để tiêu diệt hoàn toàn vi-rút và có thể gây tổn thương gan sau khi nhiễm trùng mãn tính được thiết lập. Các cytokine do cả tế bào CD4+ và CD8+ sản xuất có thể đóng một vai trò quan trọng trong cả việc ức chế sự sao chép của vi-rút và gây tổn thương gan. Hiểu rõ hơn về vai trò của miễn dịch tế bào trong quá trình sinh bệnh của nhiễm HCV có thể hỗ trợ cho việc phát triển các loại vắc-xin và các chiến lược can thiệp miễn dịch."} +{"idx": 1940081, "en": "Serological and biochemical studies indicate that acute HBV infection is resolved in the context of an efficient cell-mediated immune (CMI) response, whereas, chronic infection is characterized by weak to undetectable CMI responses and relatively efficient humoral immunity. Because humoral immunity and CMI are regulated by different TH subsets, factors which influence the induction of TH1 vs TH2 cells specific for the HBV nucleocapsid antigens (HBcAg, HBeAg) were examined in a murine model system. The factors which affected the HBc/HBeAg-specific TH1/TH2-cell balance included: (1) the structure of the antigen (i.e. HBcAg vs HBeAg); (2) the host MHC and T-cell site recognized; (3) crossregulation between TH1 and TH2 cells; (4) T-cell tolerance, which is more complete in TH1 than in TH2 cells; (5) secreted HBeAg, which preferentially depletes TH1 cells; (6) the HBV-specific subset response could be skewed towards either TH1 or TH2 predominance by cytokine treatment in vivo. The results suggest that the balance between TH1 and TH2 cells specific for the HBc/HBeAgs may be relevant in acute and chronic HBV infections. Importantly, HBeAg-specific TH2 cells preferentially evade tolerance induction as compared to their TH1-cell counterparts. Because HBeAg may act as a tolerogen during the vertical transmission of chronic HBV infection and preferentially depletes TH1 cells in the circulation, the predominance of HBeAg-specific TH2 cells may influence the initiation or maintenance of the chronic carrier state. In this case, cytokine therapy designed to shift a TH2-like response toward TH1 predominance (i.e. IL-12) may be beneficial in the treatment of chronic HBV infection.", "gemini": "Các nghiên cứu huyết thanh và sinh hóa cho thấy nhiễm HBV cấp tính được giải quyết trong bối cảnh đáp ứng miễn dịch qua trung gian tế bào (CMI) hiệu quả, trong khi nhiễm trùng mạn tính được đặc trưng bởi đáp ứng CMI yếu đến không phát hiện được và miễn dịch dịch thể tương đối hiệu quả. Vì miễn dịch dịch thể và CMI được điều chỉnh bởi các phân nhóm TH khác nhau, các yếu tố ảnh hưởng đến việc cảm ứng các tế bào TH1 so với TH2 đặc hiệu cho các kháng nguyên nucleocapsid HBV (HBcAg, HBeAg) đã được kiểm tra trong một hệ thống mô hình chuột. Các yếu tố ảnh hưởng đến sự cân bằng tế bào TH1/TH2 đặc hiệu HBc/HBeAg bao gồm: (1) cấu trúc của kháng nguyên (tức là HBcAg so với HBeAg); (2) MHC của vật chủ và vị trí tế bào T được nhận dạng; (3) sự điều hòa chéo giữa các tế bào TH1 và TH2; (4) dung nạp tế bào T, dung nạp hoàn chỉnh hơn ở tế bào TH1 so với tế bào TH2; (5) HBeAg tiết ra, làm suy giảm ưu tiên các tế bào TH1; (6) đáp ứng phân nhóm đặc hiệu HBV có thể bị lệch về ưu thế TH1 hoặc TH2 bằng cách điều trị cytokine trong cơ thể sống. Kết quả cho thấy sự cân bằng giữa các tế bào TH1 và TH2 đặc hiệu cho HBc/HBeAg có thể có liên quan trong nhiễm trùng HBV cấp tính và mạn tính. Quan trọng là các tế bào TH2 đặc hiệu HBeAg ưu tiên né tránh cảm ứng dung nạp so với các tế bào TH1 tương ứng. Vì HBeAg có thể hoạt động như một chất gây dung nạp trong quá trình lây truyền theo chiều dọc của nhiễm trùng HBV mạn tính và làm suy giảm ưu tiên các tế bào TH1 trong tuần hoàn, nên sự ưu thế của các tế bào TH2 đặc hiệu HBeAg có thể ảnh hưởng đến sự khởi đầu hoặc duy trì trạng thái mang mầm bệnh mạn tính. Trong trường hợp này, liệu pháp cytokine được thiết kế để chuyển đổi đáp ứng giống TH2 sang ưu thế TH1 (tức là IL-12) có thể có lợi trong điều trị nhiễm trùng HBV mạn tính."} +{"idx": 1940082, "en": "A library of monoclonal antibodies (MoAbs) has been produced against a human hepatocellular carcinoma (HCC) cell line designated FOCUS in order to study the antigenic properties of transformed hepatocytes. Several monoclonal antibodies (MoAbs) were initially selected for study since they bound to antigens which were overexpressed in HCC tissues compared with the adjacent uninvolved normal liver counterpart; in addition, these MoAbs revealed low level antigen expression on other normal human tissues. Subsequently, HCC cell lines were metabolically labelled and the antigens further characterized by immunoprecipitation and Western blot analysis. If the MoAb recognized a primary linear epitope on a protein, cloning was performed using a lambda GT11 cDNA expression library prepared from the FOCUS HCC cell line. These studies characterized the HCC associated antigen(s) at the molecular level. This review illustrates the value of such an experimental approach to search for and identify HCC associated antigens and emphasizes the biological properties of novel proteins may be defined and characterized by these techniques. More important, our investigations have described unique proteins that may not only be important in the pathogenesis of HCC but also demonstrates how such antigen-antibody systems may be used to develop strategies for immunotargetting and gene therapy of HCC.", "gemini": "Một thư viện các kháng thể đơn dòng (MoAb) đã được sản xuất chống lại dòng tế bào ung thư biểu mô tế bào gan ở người (HCC) có tên là FOCUS để nghiên cứu các đặc tính kháng nguyên của tế bào gan đã chuyển dạng. Một số kháng thể đơn dòng (MoAb) ban đầu được chọn để nghiên cứu vì chúng liên kết với các kháng nguyên được biểu hiện quá mức trong các mô HCC so với mô gan bình thường không liên quan liền kề; ngoài ra, các MoAb này cho thấy biểu hiện kháng nguyên mức thấp trên các mô người bình thường khác. Sau đó, các dòng tế bào HCC được đánh dấu trao đổi chất và các kháng nguyên được đặc trưng thêm bằng phương pháp miễn dịch kết tủa và phân tích Western blot. Nếu MoAb nhận ra một epitop tuyến tính chính trên protein, thì việc nhân bản được thực hiện bằng cách sử dụng thư viện biểu hiện cDNA lambda GT11 được chuẩn bị từ dòng tế bào HCC FOCUS. Các nghiên cứu này đặc trưng cho các kháng nguyên liên quan đến HCC ở cấp độ phân tử. Đánh giá này minh họa giá trị của một phương pháp tiếp cận thực nghiệm như vậy để tìm kiếm và xác định các kháng nguyên liên quan đến HCC và nhấn mạnh rằng các đặc tính sinh học của các protein mới có thể được xác định và đặc trưng bằng các kỹ thuật này. Quan trọng hơn, các cuộc điều tra của chúng tôi đã mô tả các protein độc đáo mà không chỉ có thể quan trọng trong quá trình sinh bệnh của HCC mà còn chứng minh cách các hệ thống kháng nguyên-kháng thể như vậy có thể được sử dụng để phát triển các chiến lược nhắm mục tiêu miễn dịch và liệu pháp gen của HCC."} +{"idx": 1940083, "en": "In patients with hepatitis C who have cirrhosis the rate of sustained response following interferon therapy is less than half that of patients without cirrhosis. It has been suggested, however, that a higher dose regime in patients with cirrhosis may improve response. The results of a recent Australian study of cirrhotic patients who were given an intense interferon programme of 4.5 MIU daily for 24 weeks were compared with previous studies of patients with hepatitis C. In the Australian study, 14% of patients had a sustained response at 6 months after end of therapy. Of 11 studies of interferon response in chronic hepatitis C comparison of pretreatment variables showed considerable differences. Identification of predictors of response by univariate and multivariate analysis regularly indicated the importance of age and fibrosis. Analysis of six studies with either a poor (5% or less) or a reasonable (14-19%) sustained response rate to interferon in patients with cirrhosis suggested that a higher dose or longer duration of therapy was associated with better results. The experience of the Australian study, where 14% of patients had a sustained biochemical response to interferon and side-effects were reasonably tolerated with careful monitoring, suggests that future studies in cirrhosis should be carried out exploring higher doses and longer durations of therapy.", "gemini": "Ở những bệnh nhân viêm gan C bị xơ gan, tỷ lệ đáp ứng bền vững sau liệu pháp interferon thấp hơn một nửa so với những bệnh nhân không bị xơ gan. Tuy nhiên, có ý kiến cho rằng chế độ liều cao hơn ở những bệnh nhân bị xơ gan có thể cải thiện đáp ứng. Kết quả của một nghiên cứu gần đây của Úc về những bệnh nhân bị xơ gan được tiêm một chương trình interferon chuyên sâu 4,5 MIU mỗi ngày trong 24 tuần đã được so sánh với các nghiên cứu trước đây về những bệnh nhân bị viêm gan C. Trong nghiên cứu của Úc, 14% bệnh nhân có đáp ứng bền vững ở 6 tháng sau khi kết thúc điều trị. Trong số 11 nghiên cứu về đáp ứng interferon ở bệnh viêm gan C mạn tính, so sánh các biến tiền xử lý cho thấy có sự khác biệt đáng kể. Việc xác định các yếu tố dự báo đáp ứng bằng phân tích đơn biến và đa biến thường chỉ ra tầm quan trọng của tuổi và xơ hóa. Phân tích sáu nghiên cứu có tỷ lệ đáp ứng bền vững kém (5% hoặc ít hơn) hoặc hợp lý (14-19%) với interferon ở những bệnh nhân bị xơ gan cho thấy liều cao hơn hoặc thời gian điều trị dài hơn có liên quan đến kết quả tốt hơn. Kinh nghiệm của nghiên cứu tại Úc, trong đó 14% bệnh nhân có đáp ứng sinh hóa bền vững với interferon và các tác dụng phụ được dung nạp hợp lý với sự theo dõi cẩn thận, cho thấy rằng các nghiên cứu trong tương lai về bệnh xơ gan nên được thực hiện để khám phá liều cao hơn và thời gian điều trị dài hơn."} +{"idx": 1940084, "en": "Interleukin (IL)-12 is a pleiotropic cytokine produced by antigen-presenting cells in response to diverse stimuli. IL-12 is a key molecule in the regulation of host's immune responses. In particular, IL-12 influences the balance between the T-helper cells type 1 (TH1) and type 2 (TH2); it modulates macrophage responses through the control of interferon-gamma synthesis by TH1 cells; and, suppresses IgE class antibody production (has a suppressive effect on allergic reactions) and promotes a shift in the IgG subclasses. IL-12 enhances resistance to several infectious diseases, is a powerful antitumor agent in vivo, and acts as a vaccine adjuvant. The biological properties of IL-12 point to the potential therapeutic use in persistent hepatitis B virus and hepatitis C virus infection.", "gemini": "Interleukin (IL)-12 là một cytokine đa chức năng do các tế bào trình diện kháng nguyên sản xuất để đáp ứng với các kích thích khác nhau. IL-12 là một phân tử chính trong quá trình điều hòa phản ứng miễn dịch của vật chủ. Đặc biệt, IL-12 ảnh hưởng đến sự cân bằng giữa các tế bào T trợ giúp loại 1 (TH1) và loại 2 (TH2); nó điều chỉnh phản ứng của đại thực bào thông qua việc kiểm soát tổng hợp interferon gamma của các tế bào TH1; và ức chế sản xuất kháng thể lớp IgE (có tác dụng ức chế phản ứng dị ứng) và thúc đẩy sự thay đổi trong các phân lớp IgG. IL-12 tăng cường sức đề kháng với một số bệnh truyền nhiễm, là tác nhân chống khối u mạnh trong cơ thể sống và hoạt động như một tá dược vắc-xin. Các tính chất sinh học của IL-12 cho thấy tiềm năng sử dụng trong điều trị viêm gan B mạn tính và nhiễm virus viêm gan C."} +{"idx": 1940085, "en": "Clinico-epidemiological data show that the most severe forms of hepatitis C virus (HCV) associated liver disease occur in patients with multifactorial liver damage. We found that the prevalence of hepatitis B virus (HBV) markers in anti-HCV positive patients with cirrhosis complicated by hepatocellular carcinoma (HCC) is higher than in cirrhotics with comparable age and disease history, but without HCC. HBV can persist in integrated forms in HBsAg negative, anti-HBc positive individuals and we may speculate that in such patients concurrent liver pathogens, as HCV, could cause HCC more easily than in patients without previous exposure to HBV. Analysing the relations between age at HCC diagnosis and the different risk factors in consecutive HCC patients we found that patients with single hepatitis virus infections (HBsAg and/or anti-HCV positive) were of an older median age than patients with multiple hepatitis virus infections. We also studied patients with compensated cirrhosis and hepatitis virus infections. untreated or treated with interferon-alpha. The independent effect of treatment was analysed by matching groups with regard to all the other significant HCC risk factors. The overall relative HCC risk was three times higher (risk ratio 3.1) in untreated vs treated anti-HCV positive patients and more than six times higher (risk ratio 6.2) in untreated vs treated anti-HCV positive/anti-HBc negative patients. The difference between treated and untreated patients was not statistically significant in hepatitis B surface antigen carriers and in anti-HCV positive/anti-HBc positive patients. The evidence that HBV coinfection may worsen the course of liver cirrhosis in patients with chronic hepatitis C is intriguing, but it has important practical consequences. It warrants the identification of high risk patients with chronic hepatitis C who need to be treated as early as possible and patients who can still benefit from interferon-alpha therapy once cirrhosis has already been diagnosed.", "gemini": "Dữ liệu lâm sàng-dịch tễ học cho thấy các dạng viêm gan C (HCV) nặng nhất liên quan đến bệnh gan xảy ra ở những bệnh nhân bị tổn thương gan đa yếu tố. Chúng tôi thấy rằng tỷ lệ mắc các dấu ấn viêm gan B (HBV) ở những bệnh nhân dương tính với anti-HCV bị xơ gan có biến chứng ung thư biểu mô tế bào gan (HCC) cao hơn ở những bệnh nhân bị xơ gan có tuổi và tiền sử bệnh tương đương nhưng không bị HCC. HBV có thể tồn tại ở dạng tích hợp ở những cá thể âm tính với HBsAg, dương tính với anti-HBc và chúng tôi có thể suy đoán rằng ở những bệnh nhân như vậy, các tác nhân gây bệnh gan đồng thời, như HCV, có thể gây ra HCC dễ dàng hơn ở những bệnh nhân chưa từng tiếp xúc với HBV. Khi phân tích mối quan hệ giữa tuổi chẩn đoán HCC và các yếu tố nguy cơ khác ở những bệnh nhân HCC liên tiếp, chúng tôi thấy rằng bệnh nhân bị nhiễm một loại virus viêm gan (dương tính với HBsAg và/hoặc anti-HCV) có độ tuổi trung bình cao hơn so với bệnh nhân bị nhiễm nhiều loại virus viêm gan. Chúng tôi cũng đã nghiên cứu những bệnh nhân bị xơ gan mất bù và nhiễm virus viêm gan. chưa được điều trị hoặc được điều trị bằng interferon-alpha. Tác dụng độc lập của việc điều trị được phân tích bằng cách ghép các nhóm liên quan đến tất cả các yếu tố nguy cơ HCC quan trọng khác. Nguy cơ HCC tương đối chung cao gấp ba lần (tỷ lệ nguy cơ 3,1) ở những bệnh nhân dương tính với anti-HCV không được điều trị so với những bệnh nhân được điều trị và cao hơn sáu lần (tỷ lệ nguy cơ 6,2) ở những bệnh nhân dương tính với anti-HCV/âm tính với anti-HBc không được điều trị so với những bệnh nhân được điều trị. Sự khác biệt giữa bệnh nhân được điều trị và không được điều trị không có ý nghĩa thống kê ở những người mang kháng nguyên bề mặt viêm gan B và ở những bệnh nhân dương tính với anti-HCV/dương tính với anti-HBc. Bằng chứng cho thấy nhiễm trùng đồng thời với HBV có thể làm xấu đi quá trình xơ gan ở những bệnh nhân bị viêm gan C mạn tính là rất hấp dẫn, nhưng nó có những hậu quả thực tế quan trọng. Điều này bảo đảm việc xác định những bệnh nhân có nguy cơ cao bị viêm gan C mạn tính cần được điều trị càng sớm càng tốt và những bệnh nhân vẫn có thể được hưởng lợi từ liệu pháp interferon-alpha sau khi đã được chẩn đoán mắc bệnh xơ gan."} +{"idx": 1940086, "en": "Interleukin-12 is produced by antigen-presenting cells and regulates the balance between TH1/TH2 lymphocyte subsets, promoting cell-mediated immune reactions. Amongst patients with chronic hepatitis B undergoing interferon-alpha treatment, only those who clear hepatitis B virus show a substantial increase in the production of biologically active IL-12 and an inverse ratio between serum levels of IL-12p40 subunit and IL-12. The peak of serum IL-12 occurs after the hepatitis flare and precedes or coincides with the time of HBe seroconversion. These data indicate that IL-12 is an important element for establishing the host immune control on hepatitis B virus replication in patients with chronic hepatitis B virus infection.", "gemini": "Interleukin-12 được sản xuất bởi các tế bào trình diện kháng nguyên và điều chỉnh sự cân bằng giữa các phân nhóm tế bào lympho TH1/TH2, thúc đẩy các phản ứng miễn dịch qua trung gian tế bào. Trong số những bệnh nhân bị viêm gan B mạn tính đang được điều trị bằng interferon-alpha, chỉ những bệnh nhân loại trừ được virus viêm gan B mới có sự gia tăng đáng kể về sản xuất IL-12 có hoạt tính sinh học và tỷ lệ nghịch giữa nồng độ IL-12p40 trong huyết thanh và IL-12. Nồng độ IL-12 trong huyết thanh đạt đỉnh sau khi bùng phát viêm gan và trước hoặc trùng với thời điểm chuyển đổi huyết thanh HBe. Những dữ liệu này chỉ ra rằng IL-12 là một yếu tố quan trọng để thiết lập sự kiểm soát miễn dịch của vật chủ đối với sự sao chép của virus viêm gan B ở những bệnh nhân bị nhiễm virus viêm gan B mạn tính."} +{"idx": 1940087, "en": "Lectin binding is known to be able to elicit signalling events relevant for various aspects of cell physiology. The influence of lectin binding on melanoma cells remains relatively unexplored. The aim of our study was to investigate the in vitro effects of five plant lectins, namely peanut (PNA), wheat germ (WGA), concanavalin A (Con-A), Griffonia simplicifolia (GSA-IA4) and Phaseolus vulgaris (PHA-L) agglutinins, on the cell proliferation of melanoma cell lines (SK-MEL-28, HT-144 and C32) cultured in media supplemented with either 10% or 1% fetal calf serum (FCS). Cell proliferation was assessed by means of the tetrazolium derivative reduction (MTT) assay. Four lectin concentrations were tested, namely 0.05, 0.5, 5 and 50 micrograms/ml, in four experimental settings, namely 1, 3, 5 and 7 days after the addition of each lectin to the culture media. Determination of the cell gain compartment (percentage of cells in the S and G2 phases of the cell cycle) was done by means of digital cell image analysis assessed on Feulgen-stained nuclei. Our results demonstrated that of the five lectins under study, four had a globally significant dose-dependent toxic effect on melanoma cell proliferation. The fifth lectin, PNA, had a significant stimulatory effect on the C32 cell line. Low doses of lectins may produce a transient increase in cell proliferation. Increasing the FCS from 1% to 10% in the culture media significantly antagonized lectin-induced toxicity in the three cell lines. The cell kinetics measurements showed that the inhibition of cell growth was merely due to cell death. The present data strongly suggest that some lectins might influence the proliferation of melanoma cells. In addition, because lectins are present in our diet and are able to pass into the systemic circulation, we speculate that lectins may exert an influence on melanoma growth under clinical conditions.", "gemini": "Liên kết lectin được biết là có thể gây ra các sự kiện báo hiệu có liên quan đến các khía cạnh khác nhau của sinh lý tế bào. Ảnh hưởng của liên kết lectin đối với các tế bào u hắc tố vẫn còn tương đối chưa được khám phá. Mục đích của nghiên cứu của chúng tôi là điều tra các tác động trong ống nghiệm của năm loại lectin thực vật, cụ thể là đậu phộng (PNA), mầm lúa mì (WGA), concanavalin A (Con-A), Griffonia simplicifolia (GSA-IA4) và Phaseolus vulgaris (PHA-L) agglutinin, đối với sự tăng sinh tế bào của các dòng tế bào u hắc tố (SK-MEL-28, HT-144 và C32) được nuôi cấy trong môi trường bổ sung 10% hoặc 1% huyết thanh bê thai nhi (FCS). Sự tăng sinh tế bào được đánh giá bằng phương pháp thử nghiệm khử dẫn xuất tetrazolium (MTT). Bốn nồng độ lectin đã được thử nghiệm, cụ thể là 0,05, 0,5, 5 và 50 microgam/ml, trong bốn thiết lập thực nghiệm, cụ thể là 1, 3, 5 và 7 ngày sau khi thêm từng lectin vào môi trường nuôi cấy. Xác định ngăn tăng tế bào (phần trăm tế bào trong pha S và G2 của chu kỳ tế bào) được thực hiện bằng phương pháp phân tích hình ảnh tế bào kỹ thuật số được đánh giá trên các nhân nhuộm Feulgen. Kết quả của chúng tôi chứng minh rằng trong số năm loại lectin được nghiên cứu, bốn loại có tác dụng độc hại đáng kể phụ thuộc vào liều đối với sự tăng sinh tế bào u hắc tố. Loại lectin thứ năm, PNA, có tác dụng kích thích đáng kể đối với dòng tế bào C32. Liều thấp lectin có thể làm tăng tạm thời sự tăng sinh tế bào. Tăng FCS từ 1% lên 10% trong môi trường nuôi cấy đã đối kháng đáng kể độc tính do lectin gây ra ở ba dòng tế bào. Các phép đo động học tế bào cho thấy rằng sự ức chế tăng trưởng tế bào chỉ đơn thuần là do tế bào chết. Dữ liệu hiện tại cho thấy rõ ràng rằng một số lectin có thể ảnh hưởng đến sự tăng sinh của các tế bào u hắc tố. Ngoài ra, vì lectin có trong chế độ ăn uống của chúng ta và có thể đi vào tuần hoàn hệ thống, chúng tôi suy đoán rằng lectin có thể ảnh hưởng đến sự phát triển của u hắc tố trong các điều kiện lâm sàng."} +{"idx": 1940088, "en": "The cytotoxicity of the (-)- and (+)-isomers and the quinone metabolite gossypolone prepared from the naturally occurring polyphenolic dialdehyde gossypol were compared using two human melanoma cell lines (SK-mel-19 and SK-mel-28) with a similar growth rate, one melanotic (melanin content of 69 pg/cell) and one amelanotic (melanin content of 10 pg/cell). Results from two viability assays (MTT and flow cytometry) showed that the cytotoxicity of racemic gossypol was identical for both cell lines (50% inhibition of cell growth IC50 = 22 microM). Gossypolone at equimolar concentrations was inactive in the amelanotic cell line and as potent as racemic gossypol in the melanotic cell line. (-)-Gossypol was significantly more active in both cell lines compared with the (+)-isomer. The cytotoxic effect of (-)-gossypol was both concentration and time dependent. Under serum-free conditions, the cytotoxicity of both enantiomers was increased, suggesting that serum protein binding may play a role in the differential toxicity of these isomers in vitro. Morphological changes after exposure to (-)-gossypol included shrinkage and loss of adherence. Cell sensitivity to the (-)-isomer was five-fold greater (IC50 = 4 microM) using a clonogenic assay. At equimolar concentrations, (-)-gossypol was more cytotoxic to both cell lines than the clinically used drugs cisplatin, dacarbazine and melphalan. The results of this study suggest that (-)-gossypol may be of potential therapeutic benefit in melanoma patients.", "gemini": "Độc tính tế bào của các đồng phân (-) và (+) và chất chuyển hóa quinone gossypolone được chế biến từ gossypol dialdehyde polyphenolic tự nhiên đã được so sánh bằng cách sử dụng hai dòng tế bào u hắc tố ở người (SK-mel-19 và SK-mel-28) có tốc độ tăng trưởng tương tự, một dòng có sắc tố (hàm lượng melanin là 69 pg/tế bào) và một dòng không có sắc tố (hàm lượng melanin là 10 pg/tế bào). Kết quả từ hai xét nghiệm khả thi (MTT và đo lưu lượng tế bào) cho thấy độc tính tế bào của gossypol racemic là giống nhau đối với cả hai dòng tế bào (ức chế 50% tăng trưởng tế bào IC50 = 22 microM). Gossypolone ở nồng độ tương đương không hoạt động ở dòng tế bào không có sắc tố và có hiệu lực tương đương với gossypol racemic ở dòng tế bào có sắc tố. (-)-Gossypol hoạt động mạnh hơn đáng kể ở cả hai dòng tế bào so với đồng phân (+). Tác dụng gây độc tế bào của (-)-gossypol phụ thuộc vào cả nồng độ và thời gian. Trong điều kiện không có huyết thanh, độc tính tế bào của cả hai đồng phân đều tăng lên, cho thấy rằng sự liên kết protein huyết thanh có thể đóng một vai trò trong độc tính khác biệt của các đồng phân này trong ống nghiệm. Những thay đổi về mặt hình thái sau khi tiếp xúc với (-)-gossypol bao gồm co lại và mất độ bám dính. Độ nhạy của tế bào đối với đồng phân (-) lớn hơn gấp năm lần (IC50 = 4 microM) khi sử dụng xét nghiệm tạo khuẩn lạc. Ở nồng độ tương đương, (-)-gossypol gây độc tế bào nhiều hơn đối với cả hai dòng tế bào so với các loại thuốc được sử dụng trong lâm sàng là cisplatin, dacarbazine và melphalan. Kết quả của nghiên cứu này cho thấy rằng (-)-gossypol có thể có lợi ích điều trị tiềm năng ở những bệnh nhân mắc u hắc tố."} +{"idx": 1940089, "en": "We studied the response of mouse B16F10 and SK-MEL-28 and SK-MEL-1 human melanoma cell lines to treatment with 4-hydroxyanisole (4-HA), and attempted to relate the response to the dopa oxidase levels and the morphological characteristics of each cell line. Clear dose-response curves were observed after 24 h of treatment in each cell line, the 4-HA being more toxic to the B16F10 cells, with an ID50 value of 215 microM. This was much lower than that observed for the SK-MEL-28 and SK-MEL-1 cell lines (ID50 of 5.98 mM and 7.17 mM, respectively). There was a direct relationship between toxicity levels and dopa oxidase activity, since the highest specific activity was obtained for B16F10 (15.9 mU), while lower activity was registered for SK-MEL-28 (4.59 mU) and SK-MEL-1 (1.24 mU), which also showed lower 4-HA toxicity. Morphologically, we observed the typical characteristics of cellular injury, with swelling and dilation of the internal membranes and organelles, an increased number of vacuoles, and an increased number of abnormal multilamellar melanosomes or thick clumps of irregularly distributed melanin. On the other hand, we observed that the two cell lines with the lowest dopa oxidase activity contained more mature fully melanized melanosomes than B16F10, pointing to possible alterations in the melanosome transference mechanism and lower enzymatic activity in the mature melanosomes of these two human cell lines.", "gemini": "Chúng tôi đã nghiên cứu phản ứng của các dòng tế bào u hắc tố ở người B16F10 và SK-MEL-28 và SK-MEL-1 của chuột đối với phương pháp điều trị bằng 4-hydroxyanisole (4-HA) và cố gắng liên hệ phản ứng với mức độ dopa oxidase và đặc điểm hình thái của từng dòng tế bào. Các đường cong đáp ứng liều rõ ràng đã được quan sát thấy sau 24 giờ điều trị ở mỗi dòng tế bào, 4-HA độc hơn đối với các tế bào B16F10, với giá trị ID50 là 215 microM. Giá trị này thấp hơn nhiều so với giá trị quan sát được đối với các dòng tế bào SK-MEL-28 và SK-MEL-1 (ID50 lần lượt là 5,98 mM và 7,17 mM). Có mối quan hệ trực tiếp giữa mức độ độc tính và hoạt động của dopa oxidase, vì hoạt động đặc hiệu cao nhất thu được đối với B16F10 (15,9 mU), trong khi hoạt động thấp hơn được ghi nhận đối với SK-MEL-28 (4,59 mU) và SK-MEL-1 (1,24 mU), những tế bào này cũng cho thấy độc tính 4-HA thấp hơn. Về mặt hình thái, chúng tôi quan sát thấy các đặc điểm điển hình của tổn thương tế bào, với tình trạng sưng và giãn các màng và bào quan bên trong, số lượng không bào tăng lên và số lượng melanosome đa lớp bất thường hoặc các cục melanin phân bố không đều dày lên. Mặt khác, chúng tôi quan sát thấy rằng hai dòng tế bào có hoạt động dopa oxidase thấp nhất chứa nhiều melanosome trưởng thành có sắc tố hoàn toàn hơn B16F10, chỉ ra khả năng thay đổi trong cơ chế chuyển melanosome và hoạt động của enzym thấp hơn trong các melanosome trưởng thành của hai dòng tế bào ở người này."} +{"idx": 1940090, "en": "The influence of gonadal steroids on human melanoma still remains a controversial issue. The aim of our study was to investigate whether sex steroids may influence the biological characteristics of human melanoma. Such biological characteristics were monitored at the morphological level by means of computer-assisted microscope analysis of Feulgen-stained nuclei, which provides 28 quantitative variables describing the nucleus morphometry (size, anisonucleosis level) and chromatin pattern. This methodology was used to characterize the morphonuclear features in a series of 69 human melanomas (from formalin-fixed paraffin embedded tissues) including 28 male, 17 premenopausal and 24 postmenopausal female patients, and to investigate the effect of two sex steroids (5-alpha-dihydrotestosterone [DHT] and 17-beta-oestradiol [E2]) on three human melanoma in vitro models--the HT-144, SK-MEL-28 and C32 cell lines. The results show that the morphonuclear characteristics of melanoma originating from male and female patients are very distinct (P < 0.01). This difference is still more marked (P < 0.0005) when only premenopausal female patients are compared with male patients. The in vitro data show that both DHT and E2 are able to modify markedly (P < 0.001 to P < 0.0001) the nucleus morphometry and chromatin pattern of the three cell lines. Although the mechanism and the physiological outcome are still unknown, the present work shows that there is in vivo and in vitro evidence that the biological behaviour of human melanoma is influenced by sex steroids.", "gemini": "Ảnh hưởng của steroid sinh dục đối với bệnh u hắc tố ở người vẫn là một vấn đề gây tranh cãi. Mục đích của nghiên cứu này là nhằm tìm hiểu xem steroid giới tính có thể ảnh hưởng đến các đặc điểm sinh học của bệnh u hắc tố ở người hay không. Các đặc điểm sinh học như vậy được theo dõi ở mức độ hình thái bằng cách phân tích kính hiển vi có sự hỗ trợ của máy tính đối với nhân tế bào nhuộm Feulgen, cung cấp 28 biến số định lượng mô tả hình thái nhân tế bào (kích thước, mức độ dị hướng nhân) và kiểu nhiễm sắc. Phương pháp này được sử dụng để mô tả các đặc điểm hình thái nhân của một loạt 69 bệnh u hắc tố ở người (từ các mô nhúng parafin cố định bằng formalin) bao gồm 28 bệnh nhân nam, 17 bệnh nhân nữ tiền mãn kinh và 24 bệnh nhân nữ sau mãn kinh, và để nghiên cứu tác dụng của hai steroid giới tính (5-alpha-dihydrotestosterone [DHT] và 17-beta-oestradiol [E2]) trên ba mô hình u hắc tố ở người trong ống nghiệm - dòng tế bào HT-144, SK-MEL-28 và C32. Kết quả cho thấy các đặc điểm hình thái nhân của bệnh u hắc tố có nguồn gốc từ bệnh nhân nam và nữ rất khác biệt (P < 0,01). Sự khác biệt này thậm chí còn rõ rệt hơn (P < 0,0005) khi chỉ so sánh bệnh nhân nữ tiền mãn kinh với bệnh nhân nam. Dữ liệu trong ống nghiệm cho thấy cả DHT và E2 đều có thể làm thay đổi đáng kể (P < 0,001 đến P < 0,0001) hình thái nhân và kiểu nhiễm sắc của ba dòng tế bào. Mặc dù cơ chế và kết quả sinh lý vẫn chưa được biết, nhưng công trình hiện tại cho thấy có bằng chứng trong ống nghiệm và trong ống nghiệm rằng hành vi sinh học của bệnh u hắc tố ở người bị ảnh hưởng bởi steroid giới tính."} +{"idx": 1940091, "en": "Tumour hypoxia can lead to a decrease in the biological effectiveness of radiation and alkylating agents. Few data are available on oxygen tension (PO2) in melanoma. In 20 patients with past history of melanoma, PO2 was evaluated in normal tissues and suspected metastatic lesions (nodes and skin metastases). Oxygen tension was measured using a needle probe technique (KIMOC-6650 histograph, Eppendorf, Germany), the day before the surgical removal of the suspected metastatic lesion. Histological confirmation of the malignant origin of the removed lesion was obtained in 18 cases. In two cases invasion by the known melanoma was not seen histologically. The median PO2 for normal tissues was 40.5 mmHg. For tumours, the median PO2 was 11.6 mmHg, and it was 17.1 mmHg in nodes and 6.7 mmHg in skin metastases. Very low values (< 2 mmHg) accounted for 20% of the recorded values in nodes and 15% in skin metastases. When analysed according to the node size (< or > or = 3 cm in diameter), the median PO2 was 10.4 mmHg in large nodes (six patients) and 53.3 mmHg in small nodes (six patients). For the two non-tumoral lesions, the median PO2 values were 20.9 and 25.1 mmHg, with no values below 10 mmHg. Thus a decrease in PO2 values, probably corresponding to tumour hypoxia, was found in most of the metastatic tumours when compared with normal tissues. The prognostic value of these PO2 measurements in melanoma remains to be demonstrated in the tumour response to radiotherapy or alkylating agents. However, tumour hypoxia can already be investigated as a target for new treatment modalities in metastatic melanoma.", "gemini": "Tình trạng thiếu oxy ở khối u có thể dẫn đến giảm hiệu quả sinh học của bức xạ và các tác nhân alkyl hóa. Có ít dữ liệu về độ căng oxy (PO2) trong bệnh u hắc tố. Ở 20 bệnh nhân có tiền sử u hắc tố, PO2 được đánh giá ở các mô bình thường và các tổn thương di căn nghi ngờ (di căn hạch và di căn da). Độ căng oxy được đo bằng kỹ thuật đầu dò kim (histograph KIMOC-6650, Eppendorf, Đức), một ngày trước khi phẫu thuật cắt bỏ tổn thương di căn nghi ngờ. Xác nhận mô học về nguồn gốc ác tính của tổn thương đã cắt bỏ đã thu được ở 18 trường hợp. Ở hai trường hợp, không thấy sự xâm lấn của u hắc tố đã biết về mặt mô học. Độ PO2 trung bình đối với các mô bình thường là 40,5 mmHg. Đối với khối u, độ PO2 trung bình là 11,6 mmHg, và là 17,1 mmHg ở các hạch và 6,7 mmHg ở các di căn da. Các giá trị rất thấp (< 2 mmHg) chiếm 20% các giá trị được ghi nhận ở các hạch và 15% ở các di căn da. Khi phân tích theo kích thước hạch (< hoặc > hoặc = 3 cm đường kính), độ PO2 trung bình là 10,4 mmHg ở các hạch lớn (sáu bệnh nhân) và 53,3 mmHg ở các hạch nhỏ (sáu bệnh nhân). Đối với hai tổn thương không phải khối u, các giá trị PO2 trung bình lần lượt là 20,9 và 25,1 mmHg, không có giá trị nào dưới 10 mmHg. Do đó, tình trạng giảm giá trị PO2, có thể tương ứng với tình trạng thiếu oxy ở khối u, được tìm thấy ở hầu hết các khối u di căn khi so sánh với các mô bình thường. Giá trị tiên lượng của các phép đo PO2 này trong bệnh u hắc tố vẫn cần được chứng minh trong đáp ứng của khối u với xạ trị hoặc các tác nhân alkyl hóa. Tuy nhiên, tình trạng thiếu oxy ở khối u đã có thể được nghiên cứu như một mục tiêu cho các phương thức điều trị mới trong bệnh u hắc tố di căn."} +{"idx": 1940092, "en": "The atypical naevus is both a risk factor for and a precursor lesion of melanoma. Sunlight is known to be an important aetiological factor for melanoma. Whether solar exposure is also involved in the initiation of (atypical) naevi is an issue of current interest. We performed a cross-sectional study among 270 inhabitants in the cloudy Netherlands and 282 white Dutch immigrants of the tropical island Curaçao to investigate whether solar exposure plays a role in the development of atypical naevi. All participants were interviewed and underwent total skin examination; banal melanocytic naevi and atypical naevi were counted. There was no significant difference in the mean number of melanocytic naevi > or = 2 mm or > or = 5 mm between Curaçao and the Netherlands. Furthermore, there was no significant difference in the mean crude and age standardized prevalence of atypical naevi between the Netherlands and Curaçao. In both groups individuals with atypical naevi had significantly more total naevi. Concerning the role of sun exposure in the development of naevi in the Netherlands, we found that the total naevus count had a significant association with cumulative sun exposure before the age of 12 as well as with two or more painful sunburns before the age of 12. In Curaçao these relationships were not observed. In contrast, however, on Curaçao the presence of atypical naevi showed an association (odds ratio = 2.6, 95% confidence interval 1.1-6.0) with the highest level of cumulative sun exposure and with painful sunburns before the age of 12 (odds ratio = 2.6, 95% confidence interval 1.2-5.5). In the Dutch group these associations were not significant. We hypothesize that in the development of banal naevi there is an association between the total number of naevi and sun exposure only at low exposure levels; however, after overstepping a critical threshold a further association between melanocytic naevi and sun exposure is lacking. Sunlight exposure before the age of 12 plays a complex role: only very high exposure levels seem to contribute to the development of atypical naevi.", "gemini": "Nốt ruồi bất thường vừa là yếu tố nguy cơ vừa là tổn thương tiền thân của bệnh u hắc tố. Ánh sáng mặt trời được biết là một yếu tố nguyên nhân quan trọng đối với bệnh u hắc tố. Liệu việc tiếp xúc với ánh nắng mặt trời có liên quan đến sự khởi phát của các nốt ruồi (bất thường) hay không là một vấn đề được quan tâm hiện nay. Chúng tôi đã thực hiện một nghiên cứu cắt ngang trên 270 cư dân ở vùng đất nhiều mây Hà Lan và 282 người nhập cư da trắng Hà Lan từ đảo nhiệt đới Curaçao để điều tra xem việc tiếp xúc với ánh nắng mặt trời có đóng vai trò trong sự phát triển của các nốt ruồi bất thường hay không. Tất cả những người tham gia đều được phỏng vấn và trải qua quá trình khám da toàn diện; các nốt ruồi sắc tố thông thường và các nốt ruồi bất thường đã được đếm. Không có sự khác biệt đáng kể về số lượng trung bình các nốt ruồi sắc tố> hoặc = 2 mm hoặc> hoặc = 5 mm giữa Curaçao và Hà Lan. Hơn nữa, không có sự khác biệt đáng kể về tỷ lệ mắc các nốt ruồi bất thường thô và theo tiêu chuẩn tuổi trung bình giữa Hà Lan và Curaçao. Ở cả hai nhóm, những cá nhân có nốt ruồi bất thường có tổng số nốt ruồi nhiều hơn đáng kể. Liên quan đến vai trò của việc tiếp xúc với ánh nắng mặt trời trong sự phát triển của các nốt ruồi ở Hà Lan, chúng tôi thấy rằng tổng số nốt ruồi có liên quan đáng kể với việc tiếp xúc với ánh nắng mặt trời tích lũy trước 12 tuổi cũng như với hai lần trở lên bị cháy nắng đau đớn trước 12 tuổi. Ở Curaçao, những mối quan hệ này không được quan sát thấy. Tuy nhiên, ngược lại, ở Curaçao, sự hiện diện của các nốt ruồi bất thường cho thấy mối liên quan (tỷ lệ cược = 2,6, khoảng tin cậy 95% 1,1-6,0) với mức độ tiếp xúc với ánh nắng mặt trời tích lũy cao nhất và với tình trạng cháy nắng đau đớn trước 12 tuổi (tỷ lệ cược = 2,6, khoảng tin cậy 95% 1,2-5,5). Trong nhóm người Hà Lan, những mối liên quan này không đáng kể. Chúng tôi đưa ra giả thuyết rằng trong sự phát triển của các nốt ruồi thông thường, có mối liên quan giữa tổng số nốt ruồi và việc tiếp xúc với ánh nắng mặt trời chỉ ở mức độ tiếp xúc thấp; tuy nhiên, sau khi vượt qua ngưỡng quan trọng, mối liên quan giữa các nốt ruồi sắc tố và việc tiếp xúc với ánh nắng mặt trời sẽ không còn nữa. Việc tiếp xúc với ánh nắng mặt trời trước 12 tuổi đóng một vai trò phức tạp: chỉ những mức độ tiếp xúc rất cao dường như mới góp phần vào sự phát triển của các nốt ruồi bất thường."} +{"idx": 1940093, "en": "Isolated limb perfusion (ILP) has been reported as the most effective treatment for localized recurrent melanoma of the limbs. In terms of local and regional control with melphalan, complete remission is achieved in about 40% of patients, with frequent toxicity causing significant short-term disability in many and long-term incapacity in a few. Since 1989, several studies have reported success using a combination of fotemustine and dacarbazine (DTIC) in the systemic treatment of disseminated melanoma and brain metastasis. In May 1995, we began a pilot study with systemic DTIC 400 mg/m2 given intravenously about 4 h before ILP using fotemustine in an intra-arterial bolus of 100-150 mg/m2 as the perfusion agent. By July 1996, 10 patients had been included in the study. The results were evaluated in terms of the response rate (five complete responses and one partial response in the six patients who could be evaluated), local toxicity (nine patients with level I and one with level II, measured on the Wieberdink scale) and systemic toxicity (nine patients with level 0 and one with level 1, measured on the World Health Organization scale). Treatment was effective, with a similar response rate to that obtained with melphalan but with much lower toxicity, so this protocol may represent an innovation in loco-regional therapy and should be further investigated in a large trial.", "gemini": "Liệu pháp tưới chi đơn độc (ILP) đã được báo cáo là phương pháp điều trị hiệu quả nhất đối với bệnh u hắc tố cục bộ tái phát ở chi. Về mặt kiểm soát tại chỗ và khu vực bằng melphalan, tình trạng thuyên giảm hoàn toàn đạt được ở khoảng 40% bệnh nhân, với tình trạng nhiễm độc thường xuyên gây ra tình trạng khuyết tật đáng kể trong thời gian ngắn ở nhiều bệnh nhân và tình trạng mất khả năng lâu dài ở một số ít bệnh nhân. Kể từ năm 1989, một số nghiên cứu đã báo cáo thành công khi sử dụng sự kết hợp giữa fotemustine và dacarbazine (DTIC) trong điều trị toàn thân đối với bệnh u hắc tố lan tỏa và di căn não. Vào tháng 5 năm 1995, chúng tôi đã bắt đầu một nghiên cứu thí điểm với DTIC toàn thân 400 mg/m2 tiêm tĩnh mạch khoảng 4 giờ trước khi thực hiện ILP sử dụng fotemustine trong một liều lượng lớn động mạch là 100-150 mg/m2 dưới dạng tác nhân tưới. Đến tháng 7 năm 1996, 10 bệnh nhân đã được đưa vào nghiên cứu. Kết quả được đánh giá về tỷ lệ đáp ứng (năm đáp ứng hoàn toàn và một đáp ứng một phần ở sáu bệnh nhân có thể được đánh giá), độc tính tại chỗ (chín bệnh nhân ở mức I và một bệnh nhân ở mức II, được đo trên thang Wieberdink) và độc tính toàn thân (chín bệnh nhân ở mức 0 và một bệnh nhân ở mức 1, được đo trên thang của Tổ chức Y tế Thế giới). Điều trị có hiệu quả, với tỷ lệ đáp ứng tương tự như tỷ lệ đạt được bằng melphalan nhưng độc tính thấp hơn nhiều, vì vậy phác đồ này có thể đại diện cho một đổi mới trong liệu pháp tại chỗ và khu vực và nên được nghiên cứu thêm trong một thử nghiệm lớn."} +{"idx": 1940094, "en": "In order to estimate the therapeutic activity and tolerability of immunochemotherapy with recombinant interferon-alpha 2b (rIFN-alpha 2b) plus dacarbazine (DTIC), a study was carried out in 61 patients with cytologically and/or histologically confirmed metastatic malignant melanoma. The treatment regimen was as follows: rIFN-alpha 2b 2 x 10(6) IU intramuscularly on days 1 to 4, and DTIC 800 mg/m2 intravenously on day 5, repeated at 3-week intervals until the progression of the disease or, in the case of a complete response, for up to 6 months. The overall response rate was 28%-12% complete response (CR) and 16% partial response (PR). The median response duration was 10.9 months (CR 11.5 months, PR 9.3 months; P > 0.05). Responses occurred in soft tissue and lung metastases only. The median times to treatment failure for responding and non-responding patients were 10.9 and 3 months, respectively (P < 0.0001), and the median survival durations were 16.5 and 5.8 months, respectively (P < 0.0001). The stratification of the patients into a low-risk group (World Health Organization performance status [WHO PS] < or = 1 and soft tissue or lung metastases) and a high-risk group (WHO PS = 2 or disease localization other than skin, lymph nodes or lung) showed a significant advantage for the first group with respect to the response rate, median time to treatment failure and survival duration. A flu-like syndrome was recorded in 72% of patients, nausea and vomiting in 34%, haematological toxicity in 26%, hepatic toxicity in 5%, and neurotoxicity in 5%. In view of the results obtained in our study and those reported in the literature, IFN plus DTIC immunochemotherapy represents a reasonable treatment option, particularly for patients with soft tissue and lung metastases.", "gemini": "Để ước tính hoạt động điều trị và khả năng dung nạp của liệu pháp miễn dịch hóa học với interferon alpha 2b tái tổ hợp (rIFN-alpha 2b) cộng với dacarbazine (DTIC), một nghiên cứu đã được thực hiện trên 61 bệnh nhân mắc bệnh u hắc tố ác tính di căn được xác nhận bằng tế bào học và/hoặc mô học. Phác đồ điều trị như sau: rIFN-alpha 2b 2 x 10(6) IU tiêm bắp từ ngày 1 đến ngày 4 và DTIC 800 mg/m2 tiêm tĩnh mạch vào ngày 5, lặp lại ở các khoảng thời gian 3 tuần cho đến khi bệnh tiến triển hoặc trong trường hợp đáp ứng hoàn toàn, trong thời gian tối đa 6 tháng. Tỷ lệ đáp ứng chung là 28% -12% đáp ứng hoàn toàn (CR) và 16% đáp ứng một phần (PR). Thời gian đáp ứng trung bình là 10,9 tháng (CR 11,5 tháng, PR 9,3 tháng; P > 0,05). Đáp ứng chỉ xảy ra ở các mô mềm và di căn phổi. Thời gian trung bình đến khi điều trị thất bại ở những bệnh nhân đáp ứng và không đáp ứng lần lượt là 10,9 và 3 tháng (P < 0,0001) và thời gian sống sót trung bình lần lượt là 16,5 và 5,8 tháng (P < 0,0001). Việc phân tầng bệnh nhân thành nhóm có nguy cơ thấp (trạng thái hoạt động của Tổ chức Y tế Thế giới [WHO PS] < hoặc = 1 và di căn mô mềm hoặc phổi) và nhóm có nguy cơ cao (WHO PS = 2 hoặc vị trí bệnh ngoài da, hạch bạch huyết hoặc phổi) cho thấy lợi thế đáng kể cho nhóm đầu tiên về tỷ lệ đáp ứng, thời gian trung bình đến khi điều trị thất bại và thời gian sống sót. Hội chứng giống cúm được ghi nhận ở 72% bệnh nhân, buồn nôn và nôn ở 34%, độc tính về huyết học ở 26%, độc tính về gan ở 5% và độc tính thần kinh ở 5%. Theo kết quả thu được trong nghiên cứu của chúng tôi và những kết quả được báo cáo trong tài liệu, liệu pháp miễn dịch hóa học IFN cộng với DTIC là một lựa chọn điều trị hợp lý, đặc biệt đối với những bệnh nhân bị di căn mô mềm và phổi."} +{"idx": 1940095, "en": "The objective of this study was to determine the perceptions of primary school children about sun exposure and skin cancer, and the language they use about these issues, as a basis for the design of health promotional materials. In all, 2857 children in five European countries took part in the study and were compared with 641 Australian children participating in a similar study, since the latter have been exposed to more intensive health education about the sun. The 'draw and write' technique was used. In Europe the level of awareness about the risks of excessive sun exposure and the need to protect the skin was considerably lower than in Australia, although there was some variation within northern Europe. Amongst the European children acknowledging a need to protect the skin, the principal means of protection quoted was the use of suncreams, with inadequate awareness of the value of clothing, hats and shade. European children expressed greater approval of suntans than did the Australian children. Some methodological problems were encountered as a result of nuances in the languages involved, emphasizing difficulties in international research of this type.", "gemini": "Mục tiêu của nghiên cứu này là xác định nhận thức của trẻ em tiểu học về việc tiếp xúc với ánh nắng mặt trời và ung thư da, và ngôn ngữ mà các em sử dụng về những vấn đề này, như một cơ sở cho việc thiết kế các tài liệu tuyên truyền sức khỏe. Có tổng cộng 2857 trẻ em ở năm quốc gia châu Âu tham gia nghiên cứu và được so sánh với 641 trẻ em Úc tham gia một nghiên cứu tương tự, vì những trẻ em sau đã được tiếp xúc với chương trình giáo dục sức khỏe chuyên sâu hơn về ánh nắng mặt trời. Kỹ thuật 'vẽ và viết' đã được sử dụng. Ở châu Âu, mức độ nhận thức về những rủi ro khi tiếp xúc quá nhiều với ánh nắng mặt trời và sự cần thiết phải bảo vệ da thấp hơn đáng kể so với ở Úc, mặc dù có một số khác biệt trong khu vực Bắc Âu. Trong số những trẻ em châu Âu thừa nhận cần bảo vệ da, phương tiện bảo vệ chính được trích dẫn là sử dụng kem chống nắng, với nhận thức không đầy đủ về giá trị của quần áo, mũ và bóng râm. Trẻ em châu Âu bày tỏ sự chấp thuận đối với việc tắm nắng nhiều hơn trẻ em Úc. Một số vấn đề về phương pháp luận đã gặp phải do sự khác biệt tinh tế trong các ngôn ngữ liên quan, nhấn mạnh những khó khăn trong nghiên cứu quốc tế thuộc loại này."} +{"idx": 1940096, "en": "Fourteen drug free healthy volunteers and 22 psychiatric patients under psychotropic medication were phenotyped for their individual CYP2D6 activity using dextromethorphan as a probe drug. A solution containing 20 mg dextromethorphan was administered and blood was taken 60 min later for determination of dextromethorphan and metabolites in serum. For comparison, urine was collected over 8 h after ingestion of 20 mg dextromethorphan in a separate test. The CYP2D6 phenotype was determined from the ratio of dextromethorphan to dextrorphan. For genotyping, mutant alleles of the CYP2D6 gene were identified using allele-specific polymerase chain reactions. Genotyping revealed five poor metabolizers of CYP2D6. The others were extensive metabolizers. The ratio of dextromethorphan to dextrorphan ranged from 0.01-2.53 in serum and from 0.0007-4.252 in urine. Probit analysis of serum ratios revealed a bimodal distribution with an antimode at 0.126. According to this antimode, control subjects exhibited identical phenotypes and genotypes, whereas patients under paroxetine, moclobemide or metoprolol who had been genotyped as extensive metabolizers were poor metabolizer phenotypes. Administration of tricyclic antidepressants did not change the CYP2D6 phenotype. The serum assay was more rapid and more accurate than the standard urine approach. Therefore the determination of dextromethorphan and metabolites in serum could be advantageous to measure individual CYP2D6 activities in vivo and thus optimize the dosing of drugs metabolized by CYP2D6.", "gemini": "Mười bốn tình nguyện viên khỏe mạnh không dùng thuốc và 22 bệnh nhân tâm thần đang dùng thuốc hướng thần đã được phân loại kiểu hình hoạt động CYP2D6 của từng cá nhân bằng cách sử dụng dextromethorphan làm thuốc thăm dò. Một dung dịch chứa 20 mg dextromethorphan đã được dùng và lấy máu 60 phút sau đó để xác định dextromethorphan và các chất chuyển hóa trong huyết thanh. Để so sánh, nước tiểu đã được thu thập trong vòng 8 giờ sau khi uống 20 mg dextromethorphan trong một xét nghiệm riêng biệt. Kiểu hình CYP2D6 được xác định từ tỷ lệ dextromethorphan/dextrorphan. Đối với kiểu gen, các alen đột biến của gen CYP2D6 đã được xác định bằng cách sử dụng phản ứng chuỗi polymerase đặc hiệu alen. Kiểu gen cho thấy năm người chuyển hóa kém CYP2D6. Những người khác là những người chuyển hóa mạnh. Tỷ lệ dextromethorphan/dextrorphan dao động từ 0,01-2,53 trong huyết thanh và từ 0,0007-4,252 trong nước tiểu. Phân tích Probit về tỷ lệ huyết thanh cho thấy phân bố hai đỉnh với một đỉnh ngược tại 0,126. Theo đỉnh ngược này, các đối tượng đối chứng biểu hiện kiểu hình và kiểu gen giống hệt nhau, trong khi những bệnh nhân dùng paroxetine, moclobemide hoặc metoprolol đã được xác định kiểu gen là người chuyển hóa mạnh lại có kiểu hình chuyển hóa kém. Việc sử dụng thuốc chống trầm cảm ba vòng không làm thay đổi kiểu hình CYP2D6. Xét nghiệm huyết thanh nhanh hơn và chính xác hơn so với phương pháp xét nghiệm nước tiểu tiêu chuẩn. Do đó, việc xác định dextromethorphan và các chất chuyển hóa trong huyết thanh có thể có lợi để đo hoạt động CYP2D6 ở từng cá thể trong cơ thể sống và do đó tối ưu hóa liều dùng thuốc được CYP2D6 chuyển hóa."} +{"idx": 1940097, "en": "Previous studies of associations of metabolic polymorphisms with the occurrence of malignant brain tumors have suggested that there is a significantly increased risk of development of adult gliomas in individuals who carry a poor metabolizer CYP2D6 variant allele and the GSTT1 null genotype. To investigate this further, a population-based case control study of adult glioma in the San Francisco Bay area was conducted. Patients (n = 188) diagnosed with brain tumors and controls (n = 166) were enrolled using random digit dialing and were frequency matched for age, ethnicity and gender. Genotyping for the polymorphisms was performed using standard PCR-based techniques. The analysis of the data was restricted to Caucasians because the prevalence of these traits is known to vary by ethnicity. No overall association of either the GSTT1 null genotype or CYP2D6 homozygous variant PM genotype was observed with the occurrence of brain tumors. However, when stratified by histopathologic subtype, there was a significantly increased risk for oligodendroglioma associated with the GSTT1 null genotype, with an OR of 3.2 (95% CI 1.1-9.2). These data suggest that the GSTT1 polymorphism may play a role in the development of a subset of malignant brain tumors in adults, and indicate the need for further studies.", "gemini": "Các nghiên cứu trước đây về mối liên quan của các đa hình chuyển hóa với sự xuất hiện của các khối u não ác tính đã chỉ ra rằng có nguy cơ phát triển u thần kinh đệm ở người lớn tăng đáng kể ở những cá thể mang alen biến thể CYP2D6 chuyển hóa kém và kiểu gen GSTT1 null. Để nghiên cứu sâu hơn về vấn đề này, một nghiên cứu ca chứng dựa trên dân số về u thần kinh đệm ở người lớn tại khu vực Vịnh San Francisco đã được tiến hành. Bệnh nhân (n = 188) được chẩn đoán mắc khối u não và nhóm đối chứng (n = 166) đã được tuyển dụng bằng cách quay số ngẫu nhiên và được ghép tần suất theo độ tuổi, dân tộc và giới tính. Kiểu gen cho các đa hình đã được thực hiện bằng các kỹ thuật dựa trên PCR tiêu chuẩn. Phân tích dữ liệu chỉ giới hạn ở người da trắng vì tỷ lệ mắc các đặc điểm này được biết là khác nhau tùy theo dân tộc. Không có mối liên quan chung nào của kiểu gen GSTT1 null hoặc kiểu gen PM biến thể đồng hợp tử CYP2D6 với sự xuất hiện của khối u não. Tuy nhiên, khi phân tầng theo phân nhóm mô bệnh học, có nguy cơ mắc u thần kinh đệm tăng đáng kể liên quan đến kiểu gen GSTT1 null, với OR là 3,2 (95% CI 1,1-9,2). Những dữ liệu này cho thấy rằng đa hình GSTT1 có thể đóng một vai trò trong sự phát triển của một nhóm các khối u não ác tính ở người lớn và chỉ ra sự cần thiết phải nghiên cứu sâu hơn."} +{"idx": 1940098, "en": "The relationship between a dopamine D2 receptor genetic polymorphism at the Taq1 A locus and the level of D2 receptor binding was investigated in normal, middle aged to elderly subjects with no psychiatric or neurological disorders. D2 receptor binding was measured by autoradiography in the caudate, putamen and nucleus accumbens, using the specific D2 receptor ligand [3H]-raclopride. In a sample of 44 individuals, only one was homozygous for the A1 allele, 25 were homozygous for A2 and 18 were heterozygotes. The presence of one or two A1 alleles was associated with reduced D2 receptor binding in all areas of the striatum, reaching statistical significance in the ventral caudate and putamen (p = 0.01 and p = 0.044, respectively). This reduction was more marked in males than females, particularly in the putamen. A genetic predisposition to lower D2 receptor expression may increase susceptibility to neuroleptic medication or clinical symptoms that are associated with diseases involving dopaminergic pathology.", "gemini": "Mối quan hệ giữa đa hình gen thụ thể dopamine D2 tại locus Taq1 A và mức độ liên kết thụ thể D2 đã được nghiên cứu ở những đối tượng bình thường, trung niên đến cao tuổi không mắc các rối loạn tâm thần hoặc thần kinh. Liên kết thụ thể D2 được đo bằng phương pháp chụp ảnh tự động trong nhân đuôi, vỏ não và nhân accumbens, sử dụng phối tử thụ thể D2 đặc hiệu [3H]-raclopride. Trong một mẫu gồm 44 cá thể, chỉ có một người có kiểu gen đồng hợp tử alen A1, 25 người có kiểu gen đồng hợp tử A2 và 18 người có kiểu gen dị hợp tử. Sự có mặt của một hoặc hai alen A1 có liên quan đến việc giảm liên kết thụ thể D2 ở tất cả các vùng của thể vân, đạt ý nghĩa thống kê ở đuôi bụng và vỏ não (p = 0,01 và p = 0,044). Sự giảm này rõ rệt hơn ở nam giới so với nữ giới, đặc biệt là ở vỏ não. Khuynh hướng di truyền biểu hiện thụ thể D2 thấp hơn có thể làm tăng tính nhạy cảm với thuốc an thần kinh hoặc các triệu chứng lâm sàng liên quan đến các bệnh liên quan đến bệnh lý dopaminergic."} +{"idx": 1940099, "en": "A study of the metabolism of trimethylamine was carried out in 103 healthy Thai volunteers (70 men and 33 women) and it was found that under normal dietary conditions 84-100% of trimethylamine was excreted in the urine in its N-oxide form. Five propositi living in different parts of the country were identified as having deficiency in the N-oxidation of this tertiary amine, because they excreted only 8-35% of this chemical as trimethylamine N-oxide. This metabolic defect was also confirmed by the results of an oral trimethylamine (600 mg) challenge experiment in which all five propositi were found to excrete an even smaller percentage of trimethylamine as trimethylamine N-oxide in their urine. The results of a study of the families of the two proband individuals, as well as those members of their preceding generations under normal dietary conditions, are consistent with the view that the disorder or metabolic defect is inherited in a Mendelian fashion as an autosomal recessive trait, similar to that reported for white Caucasian subjects.", "gemini": "Một nghiên cứu về quá trình chuyển hóa của trimethylamine đã được thực hiện trên 103 tình nguyện viên người Thái khỏe mạnh (70 nam và 33 nữ) và thấy rằng trong điều kiện chế độ ăn uống bình thường, 84-100% trimethylamine được bài tiết trong nước tiểu dưới dạng N-oxide. Năm propositi sống ở các vùng khác nhau của đất nước được xác định là thiếu N-oxy hóa của amin bậc ba này, vì họ chỉ bài tiết 8-35% hóa chất này dưới dạng trimethylamine N-oxide. Khiếm khuyết về chuyển hóa này cũng được xác nhận bởi kết quả của một thử nghiệm thử thách trimethylamine đường uống (600 mg), trong đó cả năm propositi đều được phát hiện bài tiết một tỷ lệ phần trăm trimethylamine thậm chí còn nhỏ hơn dưới dạng trimethylamine N-oxide trong nước tiểu của họ. Kết quả nghiên cứu trên gia đình của hai cá thể proband, cũng như những thành viên trong các thế hệ trước của họ trong điều kiện chế độ ăn uống bình thường, phù hợp với quan điểm cho rằng rối loạn hoặc khiếm khuyết chuyển hóa được di truyền theo kiểu Mendelian như một đặc điểm lặn trên nhiễm sắc thể thường, tương tự như những gì đã báo cáo đối với đối tượng da trắng da trắng."} +{"idx": 1940100, "en": "Base of tongue, eyelid, and retropharyngeal lymph node were collected from three animals experimentally infected with rinderpest and utilised in a study comparing virus isolation with histopathology, immunohistochemistry, and in situ hybridisation to determine the usefulness of the latter three techniques as retrospective diagnostic aids for this disease. Virus isolation was positive for all nine samples. Histopathology was suggestive in all the tissues and definitive in some. Immunohistochemistry and in situ hybridisation highlighted the presence of rinderpest antigen of rinderpest nucleic acid in all of the sections. However, in situ hybridisation was more specific than immunohistochemistry.", "gemini": "Đáy lưỡi, mí mắt và hạch bạch huyết retropharyngeal được thu thập từ ba con vật bị nhiễm dịch tả trâu bò thực nghiệm và được sử dụng trong một nghiên cứu so sánh việc phân lập vi-rút với mô bệnh học, miễn dịch mô hóa học và lai tại chỗ để xác định tính hữu ích của ba kỹ thuật sau như là công cụ chẩn đoán hồi cứu cho bệnh này. Phân lập vi-rút là dương tính đối với cả chín mẫu. Mô bệnh học gợi ý trong tất cả các mô và dứt khoát ở một số mô. Miễn dịch mô hóa học và lai tại chỗ làm nổi bật sự hiện diện của kháng nguyên dịch tả trâu bò của axit nucleic dịch tả trâu bò trong tất cả các phần. Tuy nhiên, lai tại chỗ đặc hiệu hơn miễn dịch mô hóa học."} +{"idx": 1940101, "en": "Neonatal mortality is a severe welfare problem in the pig industry, with the majority of neonatal deaths occurring in those piglets born later in the litter. The purpose of this study was to assess the possible link between the release of beta-endorphin, hypoxia and birth order in newborn piglets. Blood samples were taken from the umbilical cord of piglets from 16 litters at either spontaneous or cloprostenol-induced parturition. The mean (SEM) beta-endorphin concentration in cord plasma of all piglets was 276.1 (90.28)pmol litre-1 which was significantly higher than the average concentration of 73.1 (18.54)pmol litre-1 in gilts. Neither beta-endorphin nor blood gas parameters were affected by cloprostenol. A significant (P > 0.01) peak concentration of beta-endorphin was found in the piglets born four-fifths of the way through the litter. There was a significant negative correlation (P > 0.05) between cord pH and beta-endorphin concentrations. Furthermore, the concentration of beta-endorphin was positively correlated with cord pCO2 (P < 0.01). These results indicate that the release of foetal beta-endorphin is associated with the degree of acidosis in the piglets during delivery.", "gemini": "Tỷ lệ tử vong ở trẻ sơ sinh là một vấn đề phúc lợi nghiêm trọng trong ngành chăn nuôi lợn, với phần lớn các trường hợp tử vong ở trẻ sơ sinh xảy ra ở những lợn con được sinh ra muộn hơn trong lứa. Mục đích của nghiên cứu này là để đánh giá mối liên hệ có thể có giữa việc giải phóng beta-endorphin, tình trạng thiếu oxy và thứ tự sinh ở lợn con sơ sinh. Các mẫu máu được lấy từ dây rốn của lợn con từ 16 lứa đẻ tự nhiên hoặc gây đẻ bằng cloprostenol. Nồng độ beta-endorphin trung bình (SEM) trong huyết tương dây rốn của tất cả lợn con là 276,1 (90,28) pmol lít-1, cao hơn đáng kể so với nồng độ trung bình là 73,1 (18,54) pmol lít-1 ở lợn nái. Cả beta-endorphin và các thông số khí máu đều không bị ảnh hưởng bởi cloprostenol. Một nồng độ đỉnh đáng kể (P> 0,01) của beta-endorphin được tìm thấy ở những lợn con được sinh ra ở bốn phần năm của lứa. Có một mối tương quan âm đáng kể (P> 0,05) giữa độ pH dây rốn và nồng độ beta-endorphin. Hơn nữa, nồng độ beta-endorphin có tương quan dương với pCO2 dây rốn (P <0,01). Những kết quả này chỉ ra rằng việc giải phóng beta-endorphin của thai nhi có liên quan đến mức độ nhiễm toan ở lợn con trong quá trình chuyển dạ."} +{"idx": 1940102, "en": "Validation of an assay for measurement of fructosamine in equine serum and plasma utilised blood samples collected from 24 British native breed ponies. The results indicated that fructosamine can be measured easily using an assay which is precise and accurate. Paired plasma and serum fructosamine measurements were highly correlated, however, greater variations were observed within serum compared with those in plasma. A reference range for fructosamine in plasma was calculated to be 256.9 +/- 60.6 mumol litre-1 (mean +/- 2 SD). In order to assess the fructosamine response following experimental cyathostome infection, nine British native breed ponies were allocated to one of three groups: Group 1 (3.9 million third-stage cyathostome larvae (L3) over a nine-week-period), Group 2 (3.15 million L3 over seven weeks) and Group 3 (uninfected controls). From four weeks prior to infection, blood was obtained, once weekly, from all animals for measurement of plasma fructosamine, plasma albumin and serum globulin concentrations. Plasma fructosamine concentrations decreased in all infected ponies post-infection due to enteric protein loss and/or altered protein composition and/or increased protein turnover.", "gemini": "Xác nhận xét nghiệm đo fructosamine trong huyết thanh và huyết tương ngựa sử dụng các mẫu máu được thu thập từ 24 chú ngựa giống bản địa Anh. Kết quả cho thấy có thể dễ dàng đo fructosamine bằng cách sử dụng xét nghiệm có độ chính xác và chính xác. Các phép đo fructosamine trong huyết tương và huyết thanh được ghép đôi có mối tương quan cao, tuy nhiên, các biến thể lớn hơn được quan sát thấy trong huyết thanh so với trong huyết tương. Một phạm vi tham chiếu cho fructosamine trong huyết tương được tính là 256,9 +/- 60,6 mumol lít-1 (trung bình +/- 2 SD). Để đánh giá đáp ứng fructosamine sau nhiễm trùng cyathostome thực nghiệm, chín chú ngựa giống bản địa Anh đã được phân bổ vào một trong ba nhóm: Nhóm 1 (3,9 triệu ấu trùng cyathostome giai đoạn ba (L3) trong khoảng thời gian chín tuần), Nhóm 2 (3,15 triệu L3 trong bảy tuần) và Nhóm 3 (nhóm đối chứng không bị nhiễm). Bốn tuần trước khi bị nhiễm trùng, máu được lấy, một lần một tuần, từ tất cả các động vật để đo nồng độ fructosamine trong huyết tương, nồng độ albumin trong huyết tương và nồng độ globulin trong huyết thanh. Nồng độ fructosamine trong huyết tương giảm ở tất cả những chú ngựa bị nhiễm sau khi nhiễm trùng do mất protein đường ruột và/hoặc thành phần protein thay đổi và/hoặc tăng sự chuyển hóa protein."} +{"idx": 1940103, "en": "We investigated fibrin(ogen)-related antigens in liver tissues of rabbits infected with rabbit haemorrhagic disease (RHD) virus. Fibrin(ogen)-related antigens were detected in the sinusoids of liver at six hours post infection (pi). At 18 hours pi, the antigens were clearly detected in the hepatocytes infiltrated with heterophils rather than in the sinusoid. In the rabbits that spontaneously died (30 hours pi), fibrin(ogen)-related antigens were detected in both degenerating and some intact hepatocytes. They were also expressed in the necrotic foci of hepatocytes infiltrated by heterophils. By immunoprecipitation fibrin(ogen)-related antigens were extracted from the infected liver homogenates and analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot analysis. There was an increase in fibrinogen and A alpha polypeptide chains in the liver homogenates from rabbits sacrificed at 18 and 24 hours pi, including those that died. Disseminated intravascular coagulation developed after progress of degeneration and necrosis of hepatocytes in RHD. It is assumed that the resultant consumption of fibrinogen triggers replenishment of fibrinogen by hepatocytes.", "gemini": "Chúng tôi đã nghiên cứu các kháng nguyên liên quan đến fibrin (ogen) trong mô gan của thỏ bị nhiễm vi-rút bệnh xuất huyết ở thỏ (RHD). Các kháng nguyên liên quan đến fibrin (ogen) được phát hiện trong các xoang gan sau sáu giờ sau khi nhiễm trùng (pi). Vào 18 giờ pi, các kháng nguyên được phát hiện rõ ràng trong các tế bào gan bị thâm nhiễm bởi bạch cầu trung tính chứ không phải trong xoang gan. Ở những con thỏ chết tự nhiên (30 giờ pi), các kháng nguyên liên quan đến fibrin (ogen) được phát hiện trong cả tế bào gan đang thoái hóa và một số tế bào gan còn nguyên vẹn. Chúng cũng được biểu hiện ở các ổ hoại tử của tế bào gan bị bạch cầu trung tính xâm nhập. Bằng cách kết tủa miễn dịch, các kháng nguyên liên quan đến fibrin (ogen) được chiết xuất từ ​​các dịch đồng nhất gan bị nhiễm và được phân tích bằng phương pháp điện di gel polyacrylamide natri dodecyl sulfat và phân tích Western blot. Có sự gia tăng chuỗi polypeptide fibrinogen và A alpha trong các dịch đồng nhất gan từ những con thỏ bị hy sinh ở 18 và 24 giờ pi, bao gồm cả những con đã chết. Nhiễm trùng nội mạch lan tỏa phát triển sau tiến trình thoái hóa và hoại tử tế bào gan ở RHD. Người ta cho rằng sự tiêu thụ fibrinogen kết quả kích hoạt việc bổ sung fibrinogen bởi các tế bào gan."} +{"idx": 1940104, "en": "This study has identified a naturally occurring, specific deficiency of a brush border aminopeptidase N (ApN) in the small intestines of five clinically healthy dogs. ApN activity in mucosal homogenates of dog small intestine was reduced significantly in deficient animals (13.4 (1.1) nmol min-1 mg-1 protein, n = 5, P < 0.002) compared to healthy control dogs (95.1 (6.7), n = 22). Alkaline phosphatase, gamma-glutamyl transferase, zinc-resistant alpha-glucosidase, maltase, sucrase and lactase in the ApN deficient dogs exhibited comparable activities to those in the control dogs. Microvillar membranes were analysed by one- and two-dimensional electrophoresis. ApN was represented by a single 145kDa band in all control dogs, identified by immunoblotting and immunoprecipitation. Protein maps from deficient dogs were normal apart from the virtual absence of an ApN spot and there were no apparent abnormalities in the glycosylation of microvillar proteins. The findings suggest that intestinal ApN deficiency in these dogs is a primary lesion involving diminished expression of an otherwise normal enzyme protein.", "gemini": "Nghiên cứu này đã xác định được một tình trạng thiếu hụt tự nhiên, cụ thể của aminopeptidase N (ApN) ở viền nhung mao ở ruột non của năm con chó khỏe mạnh về mặt lâm sàng. Hoạt tính ApN trong dịch đồng nhất niêm mạc ruột non của chó bị giảm đáng kể ở những động vật bị thiếu hụt (13,4 (1,1) nmol min-1 mg-1 protein, n = 5, P < 0,002) so với những con chó đối chứng khỏe mạnh (95,1 (6,7), n = 22). Phosphatase kiềm, gamma-glutamyl transferase, alpha-glucosidase kháng kẽm, maltase, sucrase và lactase ở những con chó thiếu hụt ApN biểu hiện hoạt tính tương đương với những con chó đối chứng. Màng vi nhung mao được phân tích bằng phương pháp điện di một và hai chiều. ApN được biểu diễn bằng một băng đơn 145kDa ở tất cả những con chó đối chứng, được xác định bằng phương pháp miễn dịch và kết tủa miễn dịch. Bản đồ protein từ những con chó bị thiếu hụt là bình thường ngoài việc hầu như không có điểm ApN và không có bất thường rõ ràng trong quá trình glycosyl hóa của các protein vi nhung mao. Những phát hiện này cho thấy tình trạng thiếu hụt ApN ở ruột ở những con chó này là tổn thương chính liên quan đến việc biểu hiện giảm của một loại protein enzyme bình thường."} +{"idx": 1940105, "en": "This study investigated the effects of testosterone on the febrile response of castrated rams to immunological challenge. Core temperature was recorded by radiotelemetry in wethers (n = 6) injected with lipopolysaccharide endotoxin or saline before and after androgen treatment. The number of cells expressing vasopressin mRNA in the brain region implicated in the anti-pyretic response, the bed nucleus of the stria terminalis, was determined by in situ hybridisation histochemistry. Endotoxin, but not androgen, increased vasopressin message (P < 0.05), and androgen also did not alter basal or febrile temperatures. Hence, these preliminary findings question whether the androgen-dependent anti-pyretic mechanism described in the male rat is of physiological significance in the ram.", "gemini": "Nghiên cứu này đã điều tra tác động của testosterone lên phản ứng sốt của cừu đực bị thiến đối với thử thách miễn dịch. Nhiệt độ lõi được ghi lại bằng phép đo từ xa ở cừu đực (n = 6) được tiêm nội độc tố lipopolysaccharide hoặc nước muối trước và sau khi điều trị bằng androgen. Số lượng tế bào biểu hiện mRNA vasopressin trong vùng não liên quan đến phản ứng hạ sốt, nhân nền của stria terminalis, được xác định bằng mô hóa học lai in situ. Nội độc tố, nhưng không phải androgen, làm tăng thông điệp vasopressin (P < 0,05) và androgen cũng không làm thay đổi nhiệt độ cơ bản hoặc sốt. Do đó, những phát hiện ban đầu này đặt ra câu hỏi liệu cơ chế hạ sốt phụ thuộc androgen được mô tả ở chuột đực có ý nghĩa sinh lý ở cừu đực hay không."} +{"idx": 1940106, "en": "The expression of macrophage surface markers that are likely to be important in antigen presentation and cell interactions was examined in normal sheep and those with clinical paratuberculosis. Immunohistological studies demonstrated that intestinal macrophages in diseased sheep expressed MHC class II, LFA-1 and CR4 antigens weakly compared with normal tissues. Reverse transcriptase-polymerase chain reaction analysis of MHC class II mRNA in intestinal whole tissue samples showed no significant difference between control and diseased groups. A reduction in molecules such as MHC class II and LFA-1 on the surface of infected macrophages could have implications for survival of the intracellular mycobacteria and the persistence of infection.", "gemini": "Biểu hiện của các dấu hiệu bề mặt đại thực bào có khả năng quan trọng trong trình bày kháng nguyên và tương tác tế bào đã được kiểm tra ở cừu bình thường và những con mắc bệnh paratuberculosis lâm sàng. Các nghiên cứu mô miễn dịch học chứng minh rằng các đại thực bào đường ruột ở cừu mắc bệnh biểu hiện MHC lớp II, LFA-1 và kháng nguyên CR4 yếu hơn so với các mô bình thường. Phân tích phản ứng chuỗi phiên mã ngược-polymerase của mRNA MHC lớp II trong các mẫu mô toàn bộ đường ruột không cho thấy sự khác biệt đáng kể giữa nhóm đối chứng và nhóm mắc bệnh. Sự giảm các phân tử như MHC lớp II và LFA-1 trên bề mặt đại thực bào bị nhiễm trùng có thể ảnh hưởng đến sự sống sót của vi khuẩn mycobacterium trong tế bào và sự tồn tại của nhiễm trùng."} +{"idx": 1940107, "en": "As a contribution to the description of the physiological response to stress in red deer (Cervus elaphus), the potential use of the intracellular glycolytic enzyme, lactate dehydrogenase (LDH) and its isoenzyme LDH-5, as a marker of muscle damage was assessed. The distribution of LDH isoenzymes within red deer tissues was similar to that shown in other ruminants: tissue characterisation showed isoenzyme LDH-5 to be particularly associated with skeletal muscle. High plasma concentrations of creatine kinase, a muscle-specific enzyme, were associated with both high total LDH activity and the percentage of LDH-5 activity in deer undergoing a potentially stressful procedure, transportation followed by simulated abattoir lairage, which is further evidence of the specificity of the isoenzyme for skeletal muscle damage. The activity of LDH was not correlated with the plasma concentration of cortisol, a widely used physiological measure of psychological stress. This may have been due to different time courses for release and the fact that, in the present study, the immediate pre-collection procedures may have induced a short-term stress response.", "gemini": "Là một đóng góp cho việc mô tả phản ứng sinh lý đối với căng thẳng ở hươu đỏ (Cervus elaphus), khả năng sử dụng enzym glycolytic nội bào, lactate dehydrogenase (LDH) và isoenzym LDH-5 của nó, như một dấu hiệu của tổn thương cơ đã được đánh giá. Sự phân bố của các isoenzym LDH trong các mô của hươu đỏ tương tự như ở các loài nhai lại khác: đặc điểm của mô cho thấy isoenzym LDH-5 đặc biệt liên quan đến cơ xương. Nồng độ creatine kinase trong huyết tương cao, một loại enzym đặc hiệu của cơ, có liên quan đến cả hoạt động LDH toàn phần cao và tỷ lệ hoạt động LDH-5 ở hươu trải qua một thủ thuật gây căng thẳng, vận chuyển theo sau là bãi chăn nuôi mô phỏng, đây là bằng chứng thêm về tính đặc hiệu của isoenzym đối với tổn thương cơ xương. Hoạt động của LDH không tương quan với nồng độ cortisol trong huyết tương, một thước đo sinh lý được sử dụng rộng rãi về căng thẳng tâm lý. Điều này có thể là do các quá trình thời gian khác nhau để giải phóng và thực tế là, trong nghiên cứu hiện tại, các thủ tục thu thập trước ngay lập tức có thể đã gây ra phản ứng căng thẳng trong thời gian ngắn."} +{"idx": 1940108, "en": "A competitive immunoassay to quantify the serum concentration of bovine haptoglobin (hp) using time resolved fluorescence was compared with an indirect method of hp assay (haemoglobin binding assay), using sera taken from healthy animals (n = 158), animals experimentally infected with Haemophilus somnus (n = 10) and from sick animals requiring veterinary treatment (n = 440). Upper limits of normality (for normal animals) were tentatively established for the immunoassay (2.1 micrograms ml-1) and for the haemoglobin binding assay (103 micrograms ml-1). The competitive immunoassay detected elevated hp in 62.5 per cent of field sera by comparison with only 19.2 per cent using the conventional haemoglobin binding assay. Serum albumin concentration did not correlate with hp although concentrations of globulins and copper did correlate. However, these parameters (serum globulin and copper) were found to be insensitive markers of inflammatory disease.", "gemini": "Một phương pháp miễn dịch cạnh tranh để định lượng nồng độ huyết thanh của haptoglobin ở bò (hp) sử dụng huỳnh quang phân giải thời gian đã được so sánh với một phương pháp gián tiếp của xét nghiệm hp (xét nghiệm liên kết hemoglobin), sử dụng huyết thanh lấy từ động vật khỏe mạnh (n = 158), động vật bị nhiễm Haemophilus somnus theo thực nghiệm (n = 10) và từ động vật bị bệnh cần điều trị thú y (n = 440). Giới hạn trên của bình thường (đối với động vật bình thường) đã được thiết lập tạm thời cho xét nghiệm miễn dịch (2,1 microgam ml-1) và xét nghiệm liên kết hemoglobin (103 microgam ml-1). Xét nghiệm miễn dịch cạnh tranh phát hiện hp tăng ở 62,5 phần trăm huyết thanh thực địa so với chỉ 19,2 phần trăm khi sử dụng xét nghiệm liên kết hemoglobin thông thường. Nồng độ albumin huyết thanh không tương quan với hp mặc dù nồng độ globulin và đồng tương quan. Tuy nhiên, những thông số này (globulin huyết thanh và đồng) được phát hiện là những dấu hiệu không nhạy của bệnh viêm."} +{"idx": 1940109, "en": "Amplification of multiple P multocida genomic DNA fragments by outwardly-directed primers based on the repetitive extragenic palindromic (REP) consensus sequence, generated complex profiles in a PCR-based fingerprinting method known as REP-PCR. Polymorphisms within REP-PCR profiles were used to characterise 38 isolates of P multocida. The high degree of homogeneity observed among haemorrhagic septicaemia (HS) strains of serotype B and E provided evidence of a disease-associated REP profile that may serve as a novel method for the identification of HS strains regardless of serotype. REP-PCR profiles of other P multocida serotypes were highly variable, illustrating the potential of this technique for the molecular fingerprinting of fowl cholera or atrophic rhinitis isolates. A specific amplified REP fragment was isolated and used to probe membrane-bound digested P multocida genomic DNA. Hybridisation patterns not only distinguished HS-causing isolates from non-HS P multocida, but also demonstrated a degree of relatedness between HS and HS-like strains.", "gemini": "Khuếch đại nhiều đoạn DNA bộ gen P multocida bằng các mồi hướng ra ngoài dựa trên trình tự đồng thuận palindromic ngoại gen lặp lại (REP), tạo ra các cấu hình phức tạp trong một phương pháp lấy dấu vân tay dựa trên PCR được gọi là REP-PCR. Đa hình trong các cấu hình REP-PCR được sử dụng để đặc trưng 38 phân lập P multocida. Mức độ đồng nhất cao được quan sát thấy giữa các chủng hoại huyết do xuất huyết (HS) huyết thanh B và E cung cấp bằng chứng về một cấu hình REP liên quan đến bệnh có thể đóng vai trò là một phương pháp mới để xác định các chủng HS bất kể huyết thanh. Các cấu hình REP-PCR của các huyết thanh P multocida khác rất đa dạng, minh họa tiềm năng của kỹ thuật này để lấy dấu vân tay phân tử của các phân lập dịch tả gà hoặc viêm mũi teo. Một đoạn REP khuếch đại cụ thể đã được phân lập và sử dụng để thăm dò DNA bộ gen P multocida đã tiêu hóa gắn màng. Các kiểu lai không chỉ phân biệt các phân lập gây HS với P multocida không gây HS mà còn chứng minh mức độ liên quan giữa các chủng HS và HS giống."} +{"idx": 1940110, "en": "A cross-sectional multicenter study was conducted to estimate the direct costs for each cause of spinal cord injury in the United States. Random samples of 227 new injuries and 508 persons 2-16 years postinjury were selected. Prospective data were collected during one year on all charges for emergency medical services, hospitalizations, attendant care, equipment, supplies, medications, environmental modifications, physician and outpatient services, nursing homes, household assistance, vocational rehabilitation, and miscellaneous items. In 1995 dollars, first year charges averaged $233,947 for vehicle crashes, $217,868 for violence, $295,643 for sports, $185,019 for falls and $208,762 for other causes. Recurring annual charges for each cause averaged $33,439, $17,275, $27,488, $26,238 and $23,510, respectively. Using average age at time of injury for each cause, a 2% real discount rate, and the most recent survival data from the National Spinal Cord Injury Statistical Center, average lifetime charges for each cause were $969,659, $613,345, $950,973, $630,453 and $673,749, respectively. Given an estimated 10,000 new cases of spinal cord injury occurring each year of which 35.9% are caused by vehicle crashes, 29.5% are caused by violence, 20.3% are caused by falls, 7.3% are caused by sports, and 7% result from other causes, annual aggregate direct costs of traumatic spinal cord injury in the United States are $3.48 billion for vehicle crashes, $1.81 billion for violence, $1.28 billion for falls, $694 million for sports and $472 million for other causes. Total direct costs for all causes of SCI in the United States are $7.736 billion.", "gemini": "Một nghiên cứu đa trung tâm cắt ngang đã được tiến hành để ước tính chi phí trực tiếp cho từng nguyên nhân gây chấn thương tủy sống ở Hoa Kỳ. Các mẫu ngẫu nhiên gồm 227 ca chấn thương mới và 508 người từ 2-16 năm sau chấn thương đã được chọn. Dữ liệu tương lai đã được thu thập trong một năm về tất cả các chi phí cho các dịch vụ y tế khẩn cấp, nhập viện, chăm sóc người phục vụ, thiết bị, vật tư, thuốc men, cải tạo môi trường, dịch vụ bác sĩ và ngoại trú, viện dưỡng lão, hỗ trợ hộ gia đình, phục hồi nghề nghiệp và các mặt hàng khác. Theo giá đô la năm 1995, chi phí năm đầu trung bình là 233.947 đô la cho tai nạn xe cộ, 217.868 đô la cho bạo lực, 295.643 đô la cho thể thao, 185.019 đô la cho té ngã và 208.762 đô la cho các nguyên nhân khác. Chi phí hàng năm định kỳ cho mỗi nguyên nhân trung bình là 33.439 đô la, 17.275 đô la, 27.488 đô la, 26.238 đô la và 23.510 đô la. Sử dụng độ tuổi trung bình tại thời điểm chấn thương cho từng nguyên nhân, tỷ lệ chiết khấu thực là 2% và dữ liệu sống sót gần đây nhất từ Trung tâm Thống kê Chấn thương Tủy sống Quốc gia, chi phí trung bình trọn đời cho từng nguyên nhân là 969.659 đô la, 613.345 đô la, 950.973 đô la, 630.453 đô la và 673.749 đô la. Với ước tính có khoảng 10.000 ca chấn thương tủy sống mới xảy ra mỗi năm, trong đó 35,9% là do tai nạn xe cộ, 29,5% là do bạo lực, 20,3% là do ngã, 7,3% là do thể thao và 7% là do các nguyên nhân khác, tổng chi phí trực tiếp hàng năm của chấn thương tủy sống do chấn thương ở Hoa Kỳ là 3,48 tỷ đô la cho tai nạn xe cộ, 1,81 tỷ đô la cho bạo lực, 1,28 tỷ đô la cho té ngã, 694 triệu đô la cho thể thao và 472 triệu đô la cho các nguyên nhân khác. Tổng chi phí trực tiếp cho tất cả các nguyên nhân gây SCI ở Hoa Kỳ là 7,736 tỷ đô la."} +{"idx": 1940111, "en": "Three stocks of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, were propagated in bovine endothelial cells in a serum-free culture medium. The Vosloo, Welgevonden and Senegal stocks were propagated for a period of more than 203, 134, and 43 days, respectively. Two of the C ruminantium stocks (Vosloo and Senegal) were also successfully initiated under serum-free culture conditions. The serum-free medium consisted of a modified HL-1 medium. The Senegal stock was successfully propagated in Dulbecco's modified Eagle's medium nutrient mixture Ham F-12 containing 10 per cent fetal bovine serum.", "gemini": "Ba chủng Cowdria ruminantium, tác nhân gây bệnh sốt tim ở động vật nhai lại trong nước, đã được nuôi cấy trong các tế bào nội mạc ở bò trong môi trường nuôi cấy không có huyết thanh. Các chủng Vosloo, Welgevonden và Senegal đã được nuôi cấy trong thời gian hơn 203, 134 và 43 ngày. Hai trong số các chủng C ruminantium (Vosloo và Senegal) cũng đã được khởi đầu thành công trong điều kiện nuôi cấy không có huyết thanh. Môi trường không có huyết thanh bao gồm môi trường HL-1 đã được sửa đổi. Chủng Senegal đã được nuôi cấy thành công trong hỗn hợp chất dinh dưỡng Eagle's của Dulbecco đã được sửa đổi, chứa 10 phần trăm huyết thanh bò thai nhi."} +{"idx": 1940112, "en": "Previous studies have estimated that between 25% and 45% of people with spinal cord injury report severe levels of chronic pain. Few studies have examined this longitudinally. This study examines the primary pain sites, intensity and variability of perceived pain in 76 patients, 6 weeks post injury and 45 patients from the same cohort, 8 year post discharge. Demographic information reveals a close similarity with the database (40,000) from Stover and Fine's cohort (1986). Data was assessed using visual analogue scales, measures were also taken of functional independence (FIM), emotional status and coping. At 6 weeks post injury, most pain is sited in the thoracic spine area, and in the upper and lower limbs. At 1 year post discharge, most pain is reported to be in the thoracic spine area, the lumbar region and the chest. Twenty-three per cent of the 6 week group reported that the intensity of their pain was severe, whilst at 1 year, 41% of the sample complained of severe pain. Factors associated with the pain at both time points were explored using correlational analyses. The emotional, functional and psychological factors that predict pain severity were explored using multiple regression analysis. Twenty-four per cent of those reporting moderate to severe pain at 6 weeks post injury were still reporting pain at 1 year post discharge. This study examines the relative contribution of psychological factors in reported pain.", "gemini": "Các nghiên cứu trước đây ước tính rằng từ 25% đến 45% những người bị chấn thương tủy sống báo cáo mức độ đau mãn tính nghiêm trọng. Một số ít nghiên cứu đã xem xét vấn đề này theo chiều dọc. Nghiên cứu này xem xét các vị trí đau chính, cường độ và mức độ thay đổi của cơn đau được cảm nhận ở 76 bệnh nhân, 6 tuần sau chấn thương và 45 bệnh nhân từ cùng nhóm, 8 năm sau khi xuất viện. Thông tin nhân khẩu học cho thấy có sự tương đồng chặt chẽ với cơ sở dữ liệu (40.000) từ nhóm nghiên cứu của Stover và Fine (1986). Dữ liệu được đánh giá bằng thang đánh giá tương tự trực quan, các biện pháp cũng được thực hiện về khả năng độc lập về chức năng (FIM), trạng thái cảm xúc và khả năng đối phó. Vào 6 tuần sau chấn thương, hầu hết các cơn đau đều nằm ở vùng cột sống ngực và ở các chi trên và dưới. Vào 1 năm sau khi xuất viện, hầu hết các cơn đau được báo cáo là ở vùng cột sống ngực, vùng thắt lưng và ngực. Hai mươi ba phần trăm trong nhóm 6 tuần báo cáo rằng cường độ đau của họ là nghiêm trọng, trong khi ở năm 1, 41% mẫu phàn nàn về cơn đau dữ dội. Các yếu tố liên quan đến cơn đau tại cả hai thời điểm đã được khám phá bằng cách sử dụng phân tích tương quan. Các yếu tố cảm xúc, chức năng và tâm lý dự đoán mức độ nghiêm trọng của cơn đau đã được khám phá bằng cách sử dụng phân tích hồi quy đa biến. Hai mươi bốn phần trăm những người báo cáo đau vừa đến dữ dội ở 6 tuần sau chấn thương vẫn báo cáo đau ở 1 năm sau khi xuất viện. Nghiên cứu này xem xét sự đóng góp tương đối của các yếu tố tâm lý trong cơn đau được báo cáo."} +{"idx": 1940113, "en": "It has been previously demonstrated that a Brucella melitensis high-temperature-requirement A (htrA) deletion mutant is more susceptible to oxidative killing in vitro than the parental strain and is attenuated in mice. To evaluate the contribution of the B melitensis HtrA protease to virulence in ruminants, the capacity of the B melitensis htrA mutant RWP5 to produce abortion in goats was compared to that of the virulent parental strain 16M. Experimental infection with strain 16M caused abortion in eight of 12 pregnant nannies, while none of the 12 nannies inoculated with RWP5 aborted. Furthermore, intramuscular injection of fetuses in utero with RWP5 led to colonisation of the fetus with subsequent colonisation of the nanny, but no abortion was observed. Nannies vaccinated with RWP5 showed complete protection against abortion when challenged with 16M during the third trimester of pregnancy. However, these animals were not protected from colonisation by 16M. The results presented here clearly indicate that the B melitensis htrA gene product contributes to pathogenesis in goats, but the utility of B melitensis htrA mutants as vaccines in this host appears to be limited.", "gemini": "Trước đây đã chứng minh rằng đột biến xóa gen A (htrA) yêu cầu nhiệt độ cao của Brucella melitensis dễ bị tiêu diệt do oxy hóa hơn trong ống nghiệm so với chủng gốc và bị suy yếu ở chuột. Để đánh giá sự đóng góp của protease HtrA của B melitensis vào độc lực ở động vật nhai lại, khả năng gây phá thai ở dê của đột biến htrA RWP5 của B melitensis đã được so sánh với khả năng gây phá thai của chủng gốc độc lực 16M. Nhiễm trùng thực nghiệm với chủng 16M gây phá thai ở tám trong số 12 con dê cái có thai, trong khi không có con nào trong số 12 con dê cái được tiêm RWP5 bị phá thai. Hơn nữa, tiêm bắp cho thai nhi trong tử cung bằng RWP5 dẫn đến tình trạng thai nhi bị nhiễm trùng sau đó là nhiễm trùng ở dê cái, nhưng không thấy phá thai. Những con dê cái được tiêm phòng bằng RWP5 cho thấy khả năng bảo vệ hoàn toàn chống lại phá thai khi được thử thách với 16M trong tam cá nguyệt thứ ba của thai kỳ. Tuy nhiên, những con vật này không được bảo vệ khỏi tình trạng nhiễm trùng do 16M. Các kết quả trình bày ở đây chỉ ra rõ ràng rằng sản phẩm gen htrA của B melitensis góp phần gây bệnh ở dê, nhưng tác dụng của đột biến htrA của B melitensis như vắc-xin ở vật chủ này dường như bị hạn chế."} +{"idx": 1940114, "en": "The in vitro activities of valnemulin (Econor) and two other antimicrobial agents were determined against recent field strains of Mycoplasma hyopneumoniae and Mycoplasma hyosynoviae using a broth microdilution method. Valnemulin showed exceptional activity against M hyopneumoniae (MIC90 0.0005 microgram ml-1) and M hyosynoviae (MIC range 0.0001 microgram ml-1 to 0.00025 microgram ml-1) field strains. Tiamulin was 100-fold less active (MIC90 0.05 microgram ml-1) and enrofloxacin 20-fold less active (MIC90 0.01 microgram ml-1) than valnemulin against M hyopneumoniae field isolates and 20-fold to 25-fold less active (MIC range 0.0025 microgram ml-1 to 0.005 microgram ml-1) and 400-fold to 500-fold less active (MIC range 0.05 microgram ml-1 to 0.1 microgram ml-1) respectively against M hyosynoviae field isolates. No significant resistance developed to valnemulin or tiamulin in the type strain of M hyopneumoniae (strain J) or in a recent field isolate (MEVT G23) exposed to 10 in vitro passages in broths containing these antibiotics. Only slight resistance to oxytetracycline was observed. High resistance to tylosin developed in both M hyopneumoniae strains within five to seven in vitro passages in tylosin-containing broth. Providing that similar results are obtained in vivo under field conditions, valnemulin may well prove to be effective in the treatment of enzootic pneumonia and acute polyarthritis in pigs.", "gemini": "Hoạt tính trong ống nghiệm của valnemulin (Econor) và hai loại thuốc kháng khuẩn khác đã được xác định đối với các chủng Mycoplasma hyopneumoniae và Mycoplasma hyosynoviae trên đồng ruộng gần đây bằng phương pháp pha loãng vi lượng dịch truyền. Valnemulin cho thấy hoạt tính đặc biệt đối với các chủng thực địa M hyopneumoniae (MIC90 0,0005 microgam ml-1) và M hyosynoviae (phạm vi MIC từ 0,0001 microgam ml-1 đến 0,00025 microgam ml-1). Tiamulin có hoạt tính thấp hơn 100 lần (MIC90 0,05 microgam ml-1) và enrofloxacin có hoạt tính thấp hơn 20 lần (MIC90 0,01 microgam ml-1) so với valnemulin đối với các phân lập thực địa M hyopneumoniae và thấp hơn từ 20 đến 25 lần (phạm vi MIC từ 0,0025 microgam ml-1 đến 0,005 microgam ml-1) và thấp hơn từ 400 đến 500 lần (phạm vi MIC từ 0,05 microgam ml-1 đến 0,1 microgam ml-1) tương ứng đối với các phân lập thực địa M hyosynoviae. Không có khả năng kháng đáng kể nào phát triển đối với valnemulin hoặc tiamulin ở chủng loại M hyopneumoniae (chủng J) hoặc ở một phân lập thực địa gần đây (MEVT G23) tiếp xúc với 10 đoạn trong ống nghiệm trong dịch truyền chứa các loại kháng sinh này. Chỉ quan sát thấy khả năng kháng oxytetracycline nhẹ. Khả năng kháng tylosin cao đã phát triển ở cả hai chủng M hyopneumoniae trong vòng từ năm đến bảy đoạn trong ống nghiệm trong dịch truyền chứa tylosin. Với điều kiện thu được kết quả tương tự trong ống nghiệm trong điều kiện thực tế, valnemulin cũng có thể chứng minh là có hiệu quả trong điều trị viêm phổi địa phương và viêm đa khớp cấp ở lợn."} +{"idx": 1940115, "en": "Blood glutathione (GSH) concentration and the activities of enzymes associated with glutathione metabolism, namely glucose-6-phosphate dehydrogenase (G6PD) and gamma-glutamyltransferase (gamma GT) were determined in thirteen intensively-fed Simmental young bulls at seven, 10, 12.5 and 15 months of age. When G6PD activity was highest, the GSH concentration had its lowest value and inversely. This suggests that NADPH is not completely spent on glutathione reduction. gamma GT activity increased significantly during fattening.", "gemini": "Nồng độ glutathione trong máu (GSH) và hoạt động của các enzym liên quan đến quá trình chuyển hóa glutathione, cụ thể là glucose-6-phosphate dehydrogenase (G6PD) và gamma-glutamyltransferase (gamma GT) đã được xác định ở mười ba con bê non Simmental được nuôi dưỡng chuyên sâu ở bảy, 10, 12,5 và 15 tháng tuổi. Khi hoạt động của G6PD cao nhất, nồng độ GSH có giá trị thấp nhất và ngược lại. Điều này cho thấy NADPH không được sử dụng hoàn toàn để khử glutathione. Hoạt động của gamma GT tăng đáng kể trong quá trình vỗ béo."} +{"idx": 1940116, "en": "While our understanding of aging and mortality in spinal cord injury is evolving, precise estimates are still not available for expectations of life and health following a spinal cord injury. In order to derive these estimates, information about mortality and health must be combined into a single estimate. Health expectancy estimates have been widely used in the literature of the last decade to try to understand the relationship between population health and survival, both in the general population and in special populations. This study brought the benefit of this methodology to the question of long-term survival following spinal cord injury. Specifically, the study aimed to calculate life and health expectancy in a population of spinal cord injured individuals; and to estimate the effect of factors associated with survival and health. The study involved a retrospective cohort, all of whom sustained a spinal cord injury between the ages of 25 and 34 years, and between 1945 and 1990. The study predicted a median survival time of 38 years post-injury, with 43% surviving at least 40 years. These findings suggest an increase in life expectancy of about 5 years over previous research on the same cohort. Factors affecting survival were age at injury, level and completeness of lesion. Expectations of health found in the present study are similar to those found in studies of the general population. This study showed seven remaining years of poor health expected at injury, and five remaining years expected at 40 years post injury, presumably occurring at the end of life.", "gemini": "Mặc dù hiểu biết của chúng ta về lão hóa và tử vong do chấn thương tủy sống đang phát triển, nhưng vẫn chưa có ước tính chính xác về kỳ vọng về cuộc sống và sức khỏe sau chấn thương tủy sống. Để có được những ước tính này, thông tin về tỷ lệ tử vong và sức khỏe phải được kết hợp thành một ước tính duy nhất. Các ước tính về tuổi thọ khỏe mạnh đã được sử dụng rộng rãi trong các tài liệu của thập kỷ qua để cố gắng hiểu mối quan hệ giữa sức khỏe của dân số và sự sống còn, cả ở dân số nói chung và ở các nhóm dân số đặc biệt. Nghiên cứu này đã mang lại lợi ích của phương pháp luận này cho câu hỏi về khả năng sống sót lâu dài sau chấn thương tủy sống. Cụ thể, nghiên cứu này nhằm tính toán tuổi thọ và tuổi thọ khỏe mạnh trong một quần thể những người bị chấn thương tủy sống; và ước tính tác động của các yếu tố liên quan đến sự sống còn và sức khỏe. Nghiên cứu liên quan đến một nhóm đối chứng hồi cứu, tất cả đều bị chấn thương tủy sống trong độ tuổi từ 25 đến 34 và trong khoảng thời gian từ năm 1945 đến năm 1990. Nghiên cứu dự đoán thời gian sống trung bình là 38 năm sau chấn thương, với 43% sống sót ít nhất 40 năm. Những phát hiện này cho thấy tuổi thọ tăng khoảng 5 năm so với nghiên cứu trước đây trên cùng một nhóm đối chứng. Các yếu tố ảnh hưởng đến sự sống còn là tuổi khi bị thương, mức độ và mức độ hoàn chỉnh của tổn thương. Kỳ vọng về sức khỏe được tìm thấy trong nghiên cứu hiện tại tương tự như những kỳ vọng được tìm thấy trong các nghiên cứu về dân số nói chung. Nghiên cứu này cho thấy bảy năm còn lại của sức khỏe kém dự kiến khi bị thương và năm năm còn lại dự kiến ở 40 năm sau khi bị thương, có lẽ xảy ra vào cuối đời."} +{"idx": 1940117, "en": "While objective measures of impairment, disability and handicap can serve as outcome measures for the providers of medical and vocational rehabilitation services, for persons with spinal cord injury (SCI) themselves the only relevant measure of quality of life (QOL) is their own judgment as to their well-being. Subjective QOL in persons with SCI has been measured as happiness, psychological well-being morale and life satisfaction. Various studies have reported inconclusive or contradictory findings, likely due to small sample size, sample composition, measures used and other methodological issues. A meta analysis was performed to try to resolve these apparent discrepancies. A total of 22 studies with an average sample size of 102, was retrieved. Information on the relationship between QOL and impairment, disability, and handicap, if provided, was abstracted. Findings include the following: persons with SCI tend to report lower subjective well-being than non-disabled people; the relationship between impairment and QOL is weak (mean correlation: -0.05: 95% confidence interval: -0.12 to 0.02), and generally not found to be statistically significant; the association between disability and QOL is somewhat stronger (mean r: -0.21; confidence interval: -0.27 to -0.14), but not found consistently; the relationship between QOL and (aspects of) handicap is strongest (range for mean r: -0.17 to -0.48), and fairly consistently found. The number of studies available is too small to make analysis of factors that explain contradictory findings possible. Further use of subjective QOL measures in research on long-term outcomes of SCI is recommended, in order to properly reflect the perspective of the patients/clients themselves.", "gemini": "Trong khi các biện pháp đánh giá khách quan về tình trạng suy giảm chức năng, khuyết tật và tàn tật có thể đóng vai trò là các biện pháp đánh giá kết quả đối với những người cung cấp dịch vụ phục hồi chức năng y tế và nghề nghiệp, thì đối với chính những người bị chấn thương tủy sống (SCI), biện pháp đánh giá duy nhất có liên quan đến chất lượng cuộc sống (QOL) là đánh giá của chính họ về tình trạng sức khỏe của mình. QOL chủ quan ở những người bị SCI được đánh giá là hạnh phúc, sức khỏe tâm lý, tinh thần và sự hài lòng trong cuộc sống. Nhiều nghiên cứu khác nhau đã đưa ra các phát hiện không thuyết phục hoặc mâu thuẫn, có thể là do quy mô mẫu nhỏ, thành phần mẫu, biện pháp sử dụng và các vấn đề về phương pháp luận khác. Một phân tích tổng hợp đã được thực hiện để cố gắng giải quyết những mâu thuẫn rõ ràng này. Tổng cộng 22 nghiên cứu với quy mô mẫu trung bình là 102 đã được thu thập. Thông tin về mối quan hệ giữa QOL và tình trạng suy giảm chức năng, khuyết tật và tàn tật, nếu có, đã được trích xuất. Các phát hiện bao gồm: những người bị SCI có xu hướng báo cáo mức độ hạnh phúc chủ quan thấp hơn những người không khuyết tật; mối quan hệ giữa tình trạng suy giảm chức năng và QOL là yếu (hệ số tương quan trung bình: -0,05: khoảng tin cậy 95%: -0,12 đến 0,02) và thường không được tìm thấy là có ý nghĩa thống kê; mối liên hệ giữa khuyết tật và QOL mạnh hơn một chút (r trung bình: -0,21; khoảng tin cậy: -0,27 đến -0,14), nhưng không được tìm thấy một cách nhất quán; mối quan hệ giữa QOL và (các khía cạnh của) tàn tật là mạnh nhất (phạm vi r trung bình: -0,17 đến -0,48) và được tìm thấy khá nhất quán. Số lượng các nghiên cứu có sẵn là quá nhỏ để có thể phân tích các yếu tố giải thích cho các phát hiện mâu thuẫn. Nên sử dụng thêm các biện pháp QOL chủ quan trong nghiên cứu về kết quả dài hạn của SCI để phản ánh đúng quan điểm của chính bệnh nhân/khách hàng."} +{"idx": 1940118, "en": "Six healthy, spayed female Labrador Retrievers (aged 5.5 to 11 years), kept under controlled conditions of exercise and housing, were fed a diet of 15 per cent fat, 24 per cent carbohydrate and 33 per cent protein for two weeks before and between each of three test diets, of differing fat to carbohydrate ratios (A 13:44, B 20:33, C 25:26 fat:carbohydrate gm per cent dry weight) given in a predetermined order for four weeks each. Fasting plasma lipoproteins and total fatty acids were measured weekly. In the fourth week of each test period post-prandial plasma lipoproteins were measured. Diets B and C increased total (P < 0.001) and LDL cholesterol (P < 0.05) whilst diet A increased HDL triglyceride (P < 0.05). Eicosapentaenoic acid (C20:5) increased on diets B and C, and oleic acid (C18:1) on diet A. Post-prandial hypertriglyceridaemia occurred on all diets. Chylomicron clearance was slower on higher fat diets. Relatively small dietary changes produced significant alterations of plasma lipids, lipoproteins and chylomicron clearance.", "gemini": "Sáu con chó săn Labrador cái khỏe mạnh, đã được cắt bỏ buồng trứng (tuổi từ 5,5 đến 11), được nuôi trong điều kiện kiểm soát về tập thể dục và nhà ở, được cho ăn chế độ ăn có 15% chất béo, 24% carbohydrate và 33% protein trong hai tuần trước và giữa mỗi ba chế độ ăn thử nghiệm, có tỷ lệ chất béo so với carbohydrate khác nhau (A 13:44, B 20:33, C 25:26 chất béo:carbohydrate gam phần trăm trọng lượng khô) được đưa ra theo thứ tự định trước trong bốn tuần mỗi loại. Lipoprotein huyết tương lúc đói và tổng lượng axit béo được đo hàng tuần. Trong tuần thứ tư của mỗi giai đoạn thử nghiệm, lipoprotein huyết tương sau ăn đã được đo. Chế độ ăn B và C làm tăng tổng lượng cholesterol (P < 0,001) và LDL (P < 0,05) trong khi chế độ ăn A làm tăng triglycerid HDL (P < 0,05). Axit eicosapentaenoic (C20:5) tăng trên chế độ ăn B và C, và axit oleic (C18:1) trên chế độ ăn A. Tăng triglycerid máu sau ăn xảy ra trên tất cả các chế độ ăn. Độ thanh thải chylomicron chậm hơn ở chế độ ăn nhiều chất béo. Những thay đổi nhỏ trong chế độ ăn đã tạo ra những thay đổi đáng kể đối với lipid huyết tương, lipoprotein và độ thanh thải chylomicron."} +{"idx": 1940119, "en": "The major purposes of this study were to assess the quality of life (QOL) of spinal cord injured patients, and to assess the possible factors affecting the QOL. The survey was conducted from 1992-1993 by mailed questionnaires to members of Spinal Cord Injury Association of the Republic of China. There were 347 quality responses with the mean age of 37.5 +/- 10.2 years old and the mean duration of illness of 7.8 +/- 6.8 years. The questionnaire included five domains, physical mobility, environment-transportation, psychosocial adjustment, education and economics for a total of 39 items. Each item contained a rating of 'importance' and 'satisfaction'. The quality of life index (QLI) was calculated by multiplying the satisfaction score with the importance score, then dividing by the possible highest score. The major results included: (1) the subjects had mild to moderate dissatisfaction with most items in five domains except psychosocial adjustment: (2) quality of life in those with complete tetraplegia (QLI = -0.41) and incomplete tetraplegia (QLI = -0.31) was significantly lower than that of those with complete paraplegia (QLI = -0.13) and incomplete paraplegia (QLI = -0.04); (3) both the severity of injury and the post-injury working status were the major factors affecting the life quality of spinal cord injured patients in Taiwan.", "gemini": "Mục đích chính của nghiên cứu này là đánh giá chất lượng cuộc sống (QOL) của bệnh nhân chấn thương tủy sống và đánh giá các yếu tố có thể ảnh hưởng đến QOL. Khảo sát được tiến hành từ năm 1992-1993 bằng cách gửi thư có kèm bảng câu hỏi đến các thành viên của Hiệp hội chấn thương tủy sống của Trung Hoa Dân Quốc. Có 347 phản hồi chất lượng với độ tuổi trung bình là 37,5 +/- 10,2 tuổi và thời gian mắc bệnh trung bình là 7,8 +/- 6,8 năm. Bảng câu hỏi bao gồm năm lĩnh vực, khả năng vận động, môi trường-giao thông, thích ứng tâm lý xã hội, giáo dục và kinh tế với tổng cộng 39 câu hỏi. Mỗi mục chứa một đánh giá về 'tầm quan trọng' và 'sự hài lòng'. Chỉ số chất lượng cuộc sống (QLI) được tính bằng cách nhân điểm hài lòng với điểm tầm quan trọng, sau đó chia cho điểm cao nhất có thể. Các kết quả chính bao gồm: (1) các đối tượng có mức độ không hài lòng từ nhẹ đến trung bình với hầu hết các mục trong năm lĩnh vực ngoại trừ thích ứng tâm lý xã hội: (2) chất lượng cuộc sống ở những người bị liệt tứ chi hoàn toàn (QLI = -0,41) và liệt tứ chi không hoàn toàn (QLI = -0,31) thấp hơn đáng kể so với những người bị liệt nửa người hoàn toàn (QLI = -0,13) và liệt nửa người không hoàn toàn (QLI = -0,04); (3) cả mức độ nghiêm trọng của chấn thương và tình trạng làm việc sau chấn thương là những yếu tố chính ảnh hưởng đến chất lượng cuộc sống của bệnh nhân chấn thương tủy sống ở Đài Loan."} +{"idx": 1940120, "en": "The Spinal Cord Independence Measure (SCIM) is a new disability scale developed specifically for patients with spinal cord lesions in order to make the functional assessments of patients with paraplegia or tetraplegia more sensitive to changes. The SCIM includes the following areas of function: self-care (subscore (0-20), respiration and sphincter management (0-40) and mobility (0-40). Each area is scored according to its proportional weight in these patients' general activity. The final score ranges from 0 to 100. This study was performed to evaluate the reliability of the SCIM and its sensitivity to functional changes in spinal cord lesion patients compared with the Functional Independence Measure (FIM). Thirty patients were included. Scores were recorded one week after admission and thereafter every month during hospitalization. Each area of function was assessed by a pair of staff members from the relevant discipline. The comparison of scores between each pair of rates revealed a remarkable consistency (r = 0.91-0.99; P < 0.0001; slope approximately 1; constant approximately 0). The total SCIM score (mean = 51, SD = 21) was lower than the total FIM score (mean = 87, SD = 23) owing to the difference in scale range structure and the relatively high cognitive scores of our patients; however, a relationship was noted between the scores of both scales (r = 0.85, P < 0.01). The SCIM was more sensitive than the FIM to changes in function of spinal cord lesion patients: the SCIM detected all the functional changes detected by the FIM total scoring, but the FIM missed 26% of the changes detected by the SCIM total scoring. The mean difference between consecutive scores was higher for the SCIM (P < 0.01). We conclude that the SCIM is a reliable disability scale and is more sensitive to changes in function in spinal cord lesion patients than the FIM. The SCIM when administered by a multidisciplinary team, may be a useful instrument for assessing changes in everyday performance in patients with spinal cord lesion.", "gemini": "Thang Đo Độc lập Tủy sống (SCIM) là thang đo khuyết tật mới được phát triển dành riêng cho bệnh nhân tổn thương tủy sống nhằm giúp đánh giá chức năng của bệnh nhân liệt nửa người hoặc liệt tứ chi nhạy cảm hơn với các thay đổi. SCIM bao gồm các lĩnh vực chức năng sau: tự chăm sóc (điểm phụ (0-20), quản lý hô hấp và cơ vòng (0-40) và khả năng vận động (0-40). Mỗi lĩnh vực được chấm điểm theo trọng số tỷ lệ của nó trong hoạt động chung của những bệnh nhân này. Điểm cuối cùng dao động từ 0 đến 100. Nghiên cứu này được thực hiện để đánh giá độ tin cậy của SCIM và độ nhạy của nó đối với các thay đổi chức năng ở bệnh nhân tổn thương tủy sống so với Thang Đo Độc lập Chức năng (FIM). Ba mươi bệnh nhân đã được đưa vào nghiên cứu. Điểm số được ghi lại một tuần sau khi nhập viện và sau đó là hàng tháng trong thời gian nằm viện. Mỗi lĩnh vực chức năng được đánh giá bởi một cặp nhân viên từ chuyên khoa có liên quan. Việc so sánh điểm số giữa từng cặp tỷ lệ cho thấy sự nhất quán đáng kể (r = 0,91-0,99; P < 0,0001; độ dốc khoảng 1; hằng số khoảng 0). Tổng điểm SCIM (trung bình = 51, độ lệch chuẩn = 21) thấp hơn tổng điểm FIM (trung bình = 87, độ lệch chuẩn = 23) do sự khác biệt về cấu trúc phạm vi thang điểm và điểm nhận thức tương đối cao của bệnh nhân của chúng tôi; tuy nhiên, có mối quan hệ giữa điểm số của cả hai thang điểm (r = 0,85, P < 0,01). SCIM nhạy hơn FIM đối với các thay đổi về chức năng của bệnh nhân tổn thương tủy sống: SCIM phát hiện ra tất cả các thay đổi chức năng được phát hiện bởi tổng điểm FIM, nhưng FIM bỏ sót 26% các thay đổi được phát hiện bởi tổng điểm SCIM. Độ chênh lệch trung bình giữa các điểm số liên tiếp cao hơn đối với SCIM (P < 0,01). Chúng tôi kết luận rằng SCIM là thang đo khuyết tật đáng tin cậy và nhạy hơn với các thay đổi về chức năng ở bệnh nhân tổn thương tủy sống so với FIM. SCIM khi được thực hiện bởi một nhóm đa ngành có thể là một công cụ hữu ích để đánh giá các thay đổi về hiệu suất hàng ngày ở bệnh nhân tổn thương tủy sống."} +{"idx": 1940121, "en": "Ten West African Dwarf (WAD) does and 12 Djallonké ewes were artificially infected with a West African strain of Trypanosoma congolense and observed over two years. The infected animals showed a chronic anaemia together with a persistent parasitaemia but very low mortality and increase in body weights was not significantly different from the control. In the infected sheep significant differences were found in offspring production at three and five months due to a higher mortality among the lambs. The infected goats had more abortions and stillbirths and period to first kidding, total number of parturitions, production at birth, one, three and five months were significantly different from the controls. A productivity index was calculated and revealed that a chronic T congolense infection significantly decreases the productivity of WAD goats during two years whereas in Djallonké sheep, the loss in productivity is recovered after one year. Although both species are regarded as trypanotolerant, the Djallonké sheep show a better tolerance to a chronic T congolense infection that the WAD goats.", "gemini": "Mười con cừu lùn Tây Phi (WAD) và 12 con cừu Djallonké được lây nhiễm nhân tạo bằng chủng Trypanosoma congolense của Tây Phi và được theo dõi trong hai năm. Những con vật bị nhiễm bệnh biểu hiện thiếu máu mãn tính cùng với ký sinh trùng dai dẳng nhưng tỷ lệ tử vong rất thấp và sự gia tăng trọng lượng cơ thể không khác biệt đáng kể so với nhóm đối chứng. Ở những con cừu bị nhiễm bệnh, có sự khác biệt đáng kể về sản lượng con ở ba và năm tháng do tỷ lệ tử vong cao hơn ở cừu con. Những con dê bị nhiễm bệnh có nhiều trường hợp phá thai và chết lưu hơn và thời gian mang thai đầu tiên, tổng số lần sinh nở, sản lượng khi sinh, một, ba và năm tháng khác biệt đáng kể so với nhóm đối chứng. Một chỉ số năng suất đã được tính toán và cho thấy rằng nhiễm trùng T congolense mãn tính làm giảm đáng kể năng suất của dê WAD trong hai năm trong khi ở cừu Djallonké, năng suất bị mất được phục hồi sau một năm. Mặc dù cả hai loài đều được coi là chịu được bệnh trypan, nhưng cừu Djallonké cho thấy khả năng chịu đựng tốt hơn đối với nhiễm trùng T congolense mãn tính so với dê WAD."} +{"idx": 1940122, "en": "Staphylococcal resistance to a variety of antimicrobial agents, defined as inhibition of bacteria growth in the presence of an antimicrobial agent, was approximately 75 per cent among coagulase-negative staphylococci isolated from milk and the teat skin of sheep. Resistance to broad spectrum antibiotics such as chloramphenicol and tetracycline as well as resistance to the combination of trimethoprim/sulphamethoxazole commonly used for the treatment of animals, was high in both groups of isolates. Four isolates of Staphylococcus epidermidis and three Staph xylosus isolated from the milk of dairy ewes were resistant to methicillin. Methicillin resistance is prevalent among human staphylococcal isolates and resistance to this antibiotic may reflect human handling of sheep.", "gemini": "Kháng thuốc tụ cầu đối với nhiều loại thuốc kháng khuẩn, được định nghĩa là ức chế sự phát triển của vi khuẩn khi có thuốc kháng khuẩn, ở mức khoảng 75 phần trăm đối với tụ cầu âm tính với coagulase được phân lập từ sữa và da núm vú của cừu. Kháng thuốc đối với các loại kháng sinh phổ rộng như chloramphenicol và tetracycline cũng như kháng thuốc đối với sự kết hợp trimethoprim/sulphamethoxazole thường được sử dụng để điều trị động vật, đều cao ở cả hai nhóm phân lập. Bốn phân lập tụ cầu biểu bì và ba phân lập tụ cầu xylosus được phân lập từ sữa của cừu sữa kháng methicillin. Kháng methicillin rất phổ biến ở các phân lập tụ cầu ở người và tình trạng kháng thuốc này có thể phản ánh việc con người xử lý cừu."} +{"idx": 1940123, "en": "We examined the gastric emptying (GE) of oral rehydration solutions (ORS) at rest and after exercise in four Standardbred horses. In one study isotonic, cold isotonic (5 degrees C), isotonic containing glucose and hypertonic fluid were tested at rest. In another study, isotonic fluid was given following a bout of treadmill exercise at 70 per cent VO2 max until exhaustion or at rest. In both studies, a single dose of 8 litres was given via nasogastric tube. GE and electrolyte concentrations (Na+, K+ and Cl-) of the stomach content were measured at 15 minutes intervals for one hour. In both studies, 90 per cent of the fluid was emptied within 15 minutes of administration. There was no treatment effect on electrolyte concentrations in either study but significant changes did occur over time. The data showed that GE is unlikely to significantly affect rehydration.", "gemini": "Chúng tôi đã kiểm tra việc làm rỗng dạ dày (GE) của dung dịch bù nước uống (ORS) khi nghỉ ngơi và sau khi tập thể dục ở bốn con ngựa giống Standardbred. Trong một nghiên cứu, chất lỏng đẳng trương, lạnh đẳng trương (5 độ C), đẳng trương chứa glucose và chất lỏng ưu trương đã được thử nghiệm khi nghỉ ngơi. Trong một nghiên cứu khác, chất lỏng đẳng trương được đưa ra sau một cơn tập thể dục trên máy chạy bộ ở mức 70% VO2 tối đa cho đến khi kiệt sức hoặc khi nghỉ ngơi. Trong cả hai nghiên cứu, một liều duy nhất là 8 lít được đưa qua ống thông mũi dạ dày. Nồng độ GE và chất điện giải (Na +, K + và Cl-) của dịch dạ dày được đo ở các khoảng thời gian 15 phút trong một giờ. Trong cả hai nghiên cứu, 90 phần trăm chất lỏng đã được làm rỗng trong vòng 15 phút sau khi dùng. Không có tác dụng điều trị nào đối với nồng độ chất điện giải trong cả hai nghiên cứu nhưng những thay đổi đáng kể đã xảy ra theo thời gian. Dữ liệu cho thấy GE không có khả năng ảnh hưởng đáng kể đến việc bù nước."} +{"idx": 1940124, "en": "Considering biomechanics of oral implants, both loading on the implant itself and the transferred load to the bone need our attention. Mastication induces vertical and transverse forces, which induce axial forces and bending moments and exert stress gradients in the implant as well as in the bone. By the use of strain gauges or piezo-electric force transducers, one succeeds in precise intra-oral force measurements which make it possible to study a wide range of varying conditions in implant dentistry. A key determinant of the success or failure of an oral implant is the way mechanical stresses are transferred to the surrounding bone. The load transfer from implants to surrounding bone depends on the type of loading, the bone-implant interface, the length and diameter of the implants, the implant shape, structure of the implant surface, the superstructure and the quality and quality of the surrounding bone. Finite element analyses indicate maximum stress concentrations in the contact area of the implants with the cortical bone and around the apex of the implants in the trabecular bone. Although the precise mechanisms are not fully understood, it is clear that there is an adaptive remodelling response of the surrounding bone to the current situation.", "gemini": "Khi xem xét cơ sinh học của cấy ghép răng miệng, cả tải trọng lên chính implant và tải trọng truyền đến xương đều cần được chú ý. Sự nhai gây ra lực thẳng đứng và lực ngang, gây ra lực dọc trục và mô men uốn và tạo ra các gradient ứng suất trong implant cũng như trong xương. Bằng cách sử dụng máy đo ứng suất hoặc đầu dò lực áp điện, người ta thành công trong việc đo lực trong miệng chính xác, giúp nghiên cứu nhiều điều kiện khác nhau trong nha khoa cấy ghép. Một yếu tố quyết định chính đến sự thành công hay thất bại của cấy ghép răng miệng là cách ứng suất cơ học được truyền đến xương xung quanh. Sự truyền tải trọng từ implant đến xương xung quanh phụ thuộc vào loại tải, giao diện xương-implant, chiều dài và đường kính của implant, hình dạng implant, cấu trúc bề mặt implant, phần cấu trúc trên và chất lượng xương xung quanh. Các phân tích phần tử hữu hạn chỉ ra nồng độ ứng suất tối đa ở vùng tiếp xúc của implant với xương vỏ và xung quanh đỉnh của implant trong xương xốp. Mặc dù các cơ chế chính xác vẫn chưa được hiểu đầy đủ, nhưng rõ ràng là có phản ứng cải tạo thích ứng của xương xung quanh với tình hình hiện tại."} +{"idx": 1940125, "en": "Polysomnography refers to the continuous monitoring of multiple neurophysiological and cardiorespiratory variables, usually over the course of a night, to study normal and disturbed sleep. Electroencephalographic, electrooculographic and electromyographic channels provide the basis for staging the recording into successive epochs of wakefulness and various non-Rapid-Eye-Movement (REM) and REM sleep. Detection of airflow at the nose and mouth by means of thermistors, and analysis of breathing patterns recorded from sensors placed around the ribcage and abdomen, sound recordings, the electrocardiogram and the combined application of several other measurement techniques allow the assessment of normal and abnormal physiological events in relation to sleep structure. Polysomnography has an important role in the diagnosis of disorders of sleep and wakefulness as well as in the assessment of treatment effects and in scientific investigations. Developments in digital signal processing have contributed to its widespread application in clinical practice and research.", "gemini": "Đa ký giấc ngủ đề cập đến việc theo dõi liên tục nhiều biến số thần kinh sinh lý và tim mạch, thường trong suốt một đêm, để nghiên cứu giấc ngủ bình thường và bị rối loạn. Các kênh điện não đồ, điện não đồ và điện cơ đồ cung cấp cơ sở để phân đoạn bản ghi thành các thời đại liên tiếp của trạng thái thức và các giai đoạn không chuyển động mắt nhanh (REM) và giấc ngủ REM. Phát hiện luồng không khí ở mũi và miệng bằng nhiệt kế và phân tích các kiểu thở được ghi lại từ các cảm biến đặt xung quanh lồng ngực và bụng, bản ghi âm thanh, điện tâm đồ và kết hợp áp dụng một số kỹ thuật đo lường khác cho phép đánh giá các sự kiện sinh lý bình thường và bất thường liên quan đến cấu trúc giấc ngủ. Đa ký giấc ngủ có vai trò quan trọng trong chẩn đoán các rối loạn về giấc ngủ và trạng thái thức cũng như trong đánh giá hiệu quả điều trị và trong các cuộc điều tra khoa học. Sự phát triển trong xử lý tín hiệu số đã góp phần vào ứng dụng rộng rãi của nó trong thực hành lâm sàng và nghiên cứu."} +{"idx": 1940126, "en": "The primary goal of an ambulatory monitoring of motor activities (AMMA) system is to document the occurrence of random and spontaneous motor activities (e.g., sitting, lying, standing, walking, running, etc.) of the ambulatory subject in natural environmental circumstances. Much progress has been made in recording fidelity, reduction in energy requirement, fixation of the accelerometers, equipment size and weight, memory capacity and data acquisition. At present, our laboratory is interested in developing an automated off-line AMMA-signal analysis system. The system has to take care of activity (wave) detection, recognition of onsets and endpoints of the various activities (waves), and computation of a set of relevant clinical parameters (e.g., total walking time, number of times rising from a chair, etc.) from long-term recorded data. Two methods are currently being used for computerizing the off-line analysis system: using an artificial neural network and using a set of selected features extracted from the input data. The present paper is aimed at the latter method. The method was successfully applied to long-term recorded data sets of eight male amputees and three other subjects. The primary results indicate that the method is a potentially useful too to computerize the off-line analysis system.", "gemini": "Mục tiêu chính của hệ thống theo dõi vận động cơ thể (AMMA) là ghi lại sự xuất hiện của các hoạt động vận động tự phát và ngẫu nhiên (ví dụ: ngồi, nằm, đứng, đi, chạy, v.v.) của đối tượng vận động trong hoàn cảnh môi trường tự nhiên. Đã có nhiều tiến bộ trong việc ghi lại độ trung thực, giảm nhu cầu năng lượng, cố định các máy đo gia tốc, kích thước và trọng lượng thiết bị, dung lượng bộ nhớ và thu thập dữ liệu. Hiện tại, phòng thí nghiệm của chúng tôi quan tâm đến việc phát triển một hệ thống phân tích tín hiệu AMMA tự động ngoại tuyến. Hệ thống phải đảm nhiệm việc phát hiện hoạt động (sóng), nhận dạng điểm bắt đầu và điểm kết thúc của các hoạt động khác nhau (sóng) và tính toán một tập hợp các thông số lâm sàng có liên quan (ví dụ: tổng thời gian đi bộ, số lần đứng dậy khỏi ghế, v.v.) từ dữ liệu được ghi lại trong thời gian dài. Hai phương pháp hiện đang được sử dụng để vi tính hóa hệ thống phân tích ngoại tuyến: sử dụng mạng nơ-ron nhân tạo và sử dụng một tập hợp các đặc điểm được chọn trích xuất từ dữ liệu đầu vào. Bài báo hiện tại hướng đến phương pháp sau. Phương pháp này đã được áp dụng thành công cho các tập dữ liệu được ghi lại trong thời gian dài của tám người bị cắt cụt chi và ba đối tượng khác. Kết quả chính cho thấy phương pháp này có khả năng hữu ích để vi tính hóa hệ thống phân tích ngoại tuyến."} +{"idx": 1940127, "en": "OBJECTIVE To find out and analyse the events which are expected to influence the future of Intensive Care Units (ICU). DESIGN Three round Delphi study. SETTING In a first preparation round 9 events were defined. In the two rating rounds an international panel of 60 experts heads of ICUs) estimated the time when the events may come true and whether they are desired or not. RESULTS Computer tools are desired and expected in the near future (before the year 2000) for audit, quality assurance, record keeping and telecommunication; complex closed loops and nursing robots will not be used in clinical routine before 2005, they are not desired.", "gemini": "MỤC TIÊU Tìm hiểu và phân tích các sự kiện dự kiến sẽ ảnh hưởng đến tương lai của Đơn vị Chăm sóc Đặc biệt (ICU). THIẾT KẾ Nghiên cứu Delphi 3 vòng. BỐI CẢNH Trong vòng chuẩn bị đầu tiên, 9 sự kiện đã được xác định. Trong hai vòng đánh giá, một hội đồng chuyên gia quốc tế gồm 60 chuyên gia đứng đầu các đơn vị ICU đã ước tính thời điểm các sự kiện có thể trở thành sự thật và có mong muốn hay không. KẾT QUẢ Các công cụ máy tính được mong muốn và dự kiến sẽ có trong tương lai gần (trước năm 2000) để kiểm toán, đảm bảo chất lượng, lưu giữ hồ sơ và viễn thông; các vòng khép kín phức tạp và robot điều dưỡng sẽ không được sử dụng trong hoạt động lâm sàng thường quy trước năm 2005, chúng không được mong muốn."} +{"idx": 1940128, "en": "Sarcoma may arise on unabsorbable foreign bodies in rodents and more rarely in man. Perforations of the implant reduce its carcinogenicity so that nitrocellulose filters of pore size 0.45 micron have failed to induce tumour. We examined whether increase of surface area would restore carcinogenesis to film with such pore size. Nitrocellulose filters of 25 mm diameter and pore size 0.45 micron were implanted singly, in pairs and in trios subcutaneously in 3 respective groups of BALB/c mice (total 97) and observed for sarcomagenesis for 100 weeks. No tumour arose on the singles (surface area 0.98 mm2), while 7 arose on the paired (1.96 mm2) at a mean of 54 weeks and 16 on the trios (2.95 mm2) at a mean of 46 weeks (differences significant at p > 0.01). A sufficiency of surface area restores carcinogenicity to perforated foreign surfaces in mice. Surface area is dominant over film perforation size in film sarcomagenesis.", "gemini": "Sarcoma có thể phát sinh trên các dị vật không hấp thụ được ở loài gặm nhấm và hiếm khi hơn ở người. Việc đục thủng vật cấy ghép làm giảm khả năng gây ung thư của vật đó, do đó các bộ lọc nitrocellulose có kích thước lỗ 0,45 micron không gây ra khối u. Chúng tôi đã kiểm tra xem việc tăng diện tích bề mặt có khôi phục khả năng gây ung thư cho màng lọc có kích thước lỗ như vậy hay không. Các bộ lọc nitrocellulose có đường kính 25 mm và kích thước lỗ 0,45 micron được cấy đơn lẻ, theo cặp và theo bộ ba dưới da ở 3 nhóm chuột BALB/c tương ứng (tổng cộng 97) và quan sát quá trình hình thành sarcoma trong 100 tuần. Không có khối u nào phát sinh ở những con đơn lẻ (diện tích bề mặt 0,98 mm2), trong khi 7 khối u phát sinh ở những con theo cặp (1,96 mm2) với thời gian trung bình là 54 tuần và 16 khối u ở những con theo bộ ba (2,95 mm2) với thời gian trung bình là 46 tuần (sự khác biệt có ý nghĩa ở p > 0,01). Một diện tích bề mặt đủ khôi phục khả năng gây ung thư cho các bề mặt lạ bị đục lỗ ở chuột. Diện tích bề mặt chiếm ưu thế hơn kích thước lỗ thủng trên màng lọc trong quá trình hình thành sarcoma ở màng lọc."} +{"idx": 1940129, "en": "The energy expenditure and heart rate of five Shetland ponies were measured during transport and compared with the values recorded while they were at rest and walking. Secondary aims of the study were to investigate whether there was any correlation between heart rate and energy expenditure and whether different orientations affected the values to different degrees. The measurements were recorded at one-minute intervals while the ponies were at rest, walking and being transported in four different orientations, on journeys lasting 30 minutes. There were significant increases (P < 0.001) in both heart rate and energy expenditure during transport which were similar to those associated with walking, and there was a strong correlation between the two measurements. There was no conclusive evidence that orientation affected the measurements, but it appeared that rear facing transport might have had the least effect.", "gemini": "Tỷ lệ tiêu thụ năng lượng và nhịp tim của năm con ngựa Shetland đã được đo trong quá trình vận chuyển và so sánh với các giá trị được ghi lại khi chúng nghỉ ngơi và đi bộ. Mục đích thứ cấp của nghiên cứu là để tìm hiểu xem có bất kỳ mối tương quan nào giữa nhịp tim và mức tiêu thụ năng lượng hay không và liệu các hướng khác nhau có ảnh hưởng đến các giá trị ở các mức độ khác nhau hay không. Các phép đo được ghi lại ở các khoảng thời gian một phút trong khi ngựa nghỉ ngơi, đi bộ và được vận chuyển theo bốn hướng khác nhau, trong các hành trình kéo dài 30 phút. Đã có sự gia tăng đáng kể (P < 0,001) ở cả nhịp tim và mức tiêu thụ năng lượng trong quá trình vận chuyển tương tự như những trường hợp liên quan đến việc đi bộ và có mối tương quan mạnh giữa hai phép đo. Không có bằng chứng kết luận rằng hướng ảnh hưởng đến các phép đo, nhưng có vẻ như vận chuyển quay về phía sau có thể có tác động ít nhất."} +{"idx": 1940130, "en": "Forty-five calves were assigned randomly to three groups of 15 at turnout (day 0) in the spring of their first grazing season on to separate paddocks on a pasture known to carry infective larvae of Dictyocaulus viviparus. Group 1 calves were left as untreated controls, group 2 were injected with doramectin at 200 micrograms/kg at turnout and again on day 56, and group 3 were treated with an intraruminal bolus containing ivermectin. From day 42 onwards group 1 calves developed parasitic bronchitis which required repeated treatment with levamisole. The two endectocide regimes controlled lungworm infection, although some calves in group 2 developed some coughing during the week before the second dose of doramectin. After the end of the grazing season and again in May of the following year, five cattle from each group were infected experimentally with lungworm larvae and slaughtered 28 days later for lungworm counts and measurements of length to be made. At both times group 1 calves were found to be largely resistant to reinfection; group 2 were slightly more susceptible although the differences from group 1 were not statistically significant. Group 3 calves were more susceptible with no significant difference in worm counts from naive infection controls.", "gemini": "Bốn mươi lăm con bê được chỉ định ngẫu nhiên vào ba nhóm gồm 15 con khi ra ngoài (ngày 0) vào mùa xuân của mùa chăn thả đầu tiên của chúng trên các bãi chăn thả riêng biệt trên đồng cỏ được biết là mang ấu trùng truyền nhiễm của Dictyocaulus viviparus. Nhóm 1 bê được để lại làm chứng không được điều trị, nhóm 2 được tiêm doramectin với liều 200 microgam/kg khi ra ngoài và một lần nữa vào ngày 56 và nhóm 3 được điều trị bằng viên thuốc đặt vào dạ dày chứa ivermectin. Từ ngày 42 trở đi, bê nhóm 1 phát triển viêm phế quản do ký sinh trùng cần phải điều trị nhiều lần bằng levamisole. Hai chế độ nội tiết tố kiểm soát nhiễm trùng giun phổi, mặc dù một số bê ở nhóm 2 bị ho trong tuần trước khi dùng liều doramectin thứ hai. Sau khi kết thúc mùa chăn thả và một lần nữa vào tháng 5 năm sau, năm con gia súc từ mỗi nhóm được nhiễm thử nghiệm bằng ấu trùng giun phổi và giết mổ 28 ngày sau đó để đếm giun phổi và đo chiều dài. Cả hai lần bê nhóm 1 đều được phát hiện có khả năng chống lại tái nhiễm; nhóm 2 dễ bị nhiễm hơn một chút mặc dù sự khác biệt so với nhóm 1 không có ý nghĩa thống kê. Bê nhóm 3 dễ bị nhiễm hơn mà không có sự khác biệt đáng kể về số lượng giun so với chứng nhiễm ngẫu nhiên."} +{"idx": 1940131, "en": "This paper describes the clinical and histopathological features of an outbreak of cutaneous squamous cell carcinomas in a flock of Berrichon du Cher sheep in south-western Spain. The outbreak was unusual because of the high prevalence recorded (15 to 18 per cent over the last two years) and because the majority of the lesions involved the eyelids. Adult and old sheep were the most commonly affected, and the largest numbers of cases were recorded in summer and autumn. The initial lesions were non-specific and consisted of erythema, hyperkeratosis, actinic keratosis, periocular dermatitis and conjunctivitis. The tumours grew slowly but progressively over a period of one to two years, with frequent complications by bacterial infections or secondary myiasis. Histopathological examination of the tumours revealed well-differentiated squamous cell carcinomas, surrounded by a moderate to abundant infiltrate of lymphocytes, plasma cells and macrophages. The overlying epidermis was frequently ulcerated and inflamed. The possible relationships between the high prevalence of the condition and the poorly pigmented skin of this breed, the extensive farming system employed and the exposure of the animals to ultraviolet radiation were analysed.", "gemini": "Bài báo này mô tả các đặc điểm lâm sàng và mô bệnh học của một đợt bùng phát ung thư biểu mô tế bào vảy ở da trong một đàn cừu Berrichon du Cher ở tây nam Tây Ban Nha. Đợt bùng phát này không bình thường vì tỷ lệ mắc cao được ghi nhận (từ 15 đến 18 phần trăm trong hai năm qua) và vì phần lớn các tổn thương liên quan đến mí mắt. Cừu trưởng thành và già là những con thường bị ảnh hưởng nhất, và số lượng trường hợp lớn nhất được ghi nhận vào mùa hè và mùa thu. Các tổn thương ban đầu không đặc hiệu và bao gồm ban đỏ, tăng sừng hóa, sừng hóa do ánh sáng mặt trời, viêm da quanh mắt và viêm kết mạc. Các khối u phát triển chậm nhưng tiến triển trong khoảng thời gian từ một đến hai năm, thường có biến chứng do nhiễm trùng vi khuẩn hoặc nhiễm trùng thứ phát. Kiểm tra mô bệnh học của các khối u cho thấy ung thư biểu mô tế bào vảy biệt hóa tốt, xung quanh có sự thâm nhiễm vừa phải đến nhiều tế bào lympho, tế bào plasma và đại thực bào. Lớp biểu bì bên trên thường bị loét và viêm. Các mối quan hệ có thể có giữa tỷ lệ mắc cao của tình trạng này và làn da ít sắc tố của giống này, hệ thống chăn thả rộng lớn được sử dụng và việc tiếp xúc của động vật với bức xạ cực tím đã được phân tích."} +{"idx": 1940132, "en": "Implantation of a transjugular intrahepatic portosystemic shunt (TIPS) is associated with a broad spectrum of acute and chronic complications. Data concerning incidence and prognosis of these complications are conflicting but of great importance toward defining the role of TIPS relative to other therapeutic options. We conducted a prospective, uncontrolled cohort study in 53 patients to assess incidence, management and clinical outcome of complications occurring after TIPS procedure. Mean follow-up was 21.1 +/- 9.0 months. Technique-related mortality was 2%; 9% of patients died within 30 days after TIPS procedure. The overall survival rate after 18 months was 74%. The overall incidence of primary hepatic encephalopathy (HE) within the first year was 25%, and 77% of episodes could be managed successfully by medical treatment or implantation of a reducing stent. The rate of patients without rebleeding after 18 months was 84%. Rebleeding was associated with shunt abnormalities, and the bleeding was controlled by revision of the stent. Two patients died of variceal hemorrhage. The cumulative incidence of shunt stenosis or occlusion was 47% after 18 months. The technical success rate of shunt revision was 97%. TIPS implantation is associated with a considerable risk of HE and shunt stenosis or occlusion. Nevertheless most episodes of HE can be managed by medical treatment or implantation of a reducing stent. Angiographic revision of the stent is successful in nearly all cases of stenosis or occlusion. We therefore conclude that TIPS implantation in combination with careful follow-up examinations constitutes effective medium-term treatment of portal hypertension in a considerable proportion of patients.", "gemini": "Việc cấy ghép đường nối cửa chủ trong gan qua tĩnh mạch cảnh (TIPS) có liên quan đến nhiều biến chứng cấp tính và mãn tính. Dữ liệu liên quan đến tỷ lệ mắc và tiên lượng của các biến chứng này còn mâu thuẫn nhưng lại có tầm quan trọng lớn đối với việc xác định vai trò của TIPS so với các lựa chọn điều trị khác. Chúng tôi đã tiến hành một nghiên cứu đoàn hệ không đối chứng, có triển vọng ở 53 bệnh nhân để đánh giá tỷ lệ mắc, cách xử trí và kết cục lâm sàng của các biến chứng xảy ra sau thủ thuật TIPS. Thời gian theo dõi trung bình là 21,1 +/- 9,0 tháng. Tỷ lệ tử vong liên quan đến kỹ thuật là 2%; 9% bệnh nhân tử vong trong vòng 30 ngày sau thủ thuật TIPS. Tỷ lệ sống sót chung sau 18 tháng là 74%. Tỷ lệ mắc bệnh não gan (HE) tiên phát trong năm đầu tiên là 25%, và 77% các đợt có thể được xử trí thành công bằng điều trị nội khoa hoặc cấy ghép stent giảm áp. Tỷ lệ bệnh nhân không tái chảy máu sau 18 tháng là 84%. Tái chảy máu có liên quan đến bất thường của đường nối và tình trạng chảy máu được kiểm soát bằng cách sửa đổi stent. Hai bệnh nhân tử vong vì xuất huyết giãn tĩnh mạch. Tỷ lệ mắc tắc nghẽn hoặc hẹp đường nối tích lũy là 47% sau 18 tháng. Tỷ lệ thành công về mặt kỹ thuật của việc sửa đổi đường nối là 97%. Cấy ghép TIPS có liên quan đến nguy cơ đáng kể về HE và hẹp hoặc tắc nghẽn đường nối. Tuy nhiên, hầu hết các đợt HE có thể được xử trí bằng điều trị nội khoa hoặc cấy ghép stent giảm áp. Sửa đổi đường nối bằng chụp mạch thành công trong hầu hết các trường hợp hẹp hoặc tắc nghẽn. Do đó, chúng tôi kết luận rằng cấy ghép TIPS kết hợp với các xét nghiệm theo dõi cẩn thận cấu thành phương pháp điều trị trung hạn hiệu quả đối với tăng áp lực tĩnh mạch cửa ở một tỷ lệ đáng kể bệnh nhân."} +{"idx": 1940133, "en": "Dysphagia, of a cardiac origin, is a generally accepted, yet rarely diagnosed symptom. A 84-year-old female patient with left atrial dilatation, presenting with dysphagia and weight loss, was treated for global heart failure. Esophagography revealed compression of the distal esophagus. Echocardiography showed a left atrial, right atrial and right ventricular enlargement. Esophageal manometry revealed hypotonic peristaltic contractions of the esophageal body and a lower than normal resting pressure of the lower esophageal sphincter. After medical treatment for myocardial insufficiency and of esophageal motility, the clinical signs of global heart failure were improved and the dysphagia disappeared. Following the exclusion of common causes of dysphagia in patients with heart failure, dysphagia due to left atrial dilatation should be considered.", "gemini": "Khó nuốt có nguồn gốc từ tim là một triệu chứng thường được chấp nhận nhưng lại ít khi được chẩn đoán. Một bệnh nhân nữ 84 tuổi bị giãn nhĩ trái, biểu hiện khó nuốt và sụt cân, được điều trị suy tim toàn thể. Chụp thực quản cho thấy tình trạng chèn ép thực quản xa. Siêu âm tim cho thấy tình trạng giãn nhĩ trái, nhĩ phải và thất phải. Đo áp lực thực quản cho thấy các cơn co thắt nhu động hạ huyết áp của thân thực quản và áp lực nghỉ của cơ thắt thực quản dưới thấp hơn bình thường. Sau khi điều trị nội khoa tình trạng suy cơ tim và nhu động thực quản, các dấu hiệu lâm sàng của suy tim toàn thể đã được cải thiện và tình trạng khó nuốt đã biến mất. Sau khi loại trừ các nguyên nhân phổ biến gây khó nuốt ở những bệnh nhân bị suy tim, cần xem xét tình trạng khó nuốt do giãn nhĩ trái."} +{"idx": 1940134, "en": "The design of the PCR system presented in this work is based on the knowledge of the molecular character of the crown gall disease. The virulence of Agrobacterium tumefaciens requires the presence of a big (up to 235,000 bp) plasmid Ti (pTi-tumour inducing plasmid). This plasmid carries the so-called T-DNA fragment (T-DNA-transferred DNA), which integrates into cell chromosomes of the infected plants and subsequently changes the plant morphology nad metabolism. In cannot be excluded that after T-DNA integration the presence of Agrobacterium is not necessary for the development of pathological changes. Thus, T-DNA is the only sign that must be present both in virulent bacteria and in infected plants in any stadium of the disease and even before the infection. This is why T-DNA was chosen as the target region for PCR amplification. Primers flanking a 220 bp fragment of one of the conservative regions responsible for Agrobacterium pathogenicity, namely tms2 gene coding for indolacetamide amidohydrolase (the second step of auxin biosynthesis) were designed as the optimal for PCR amplification. The PCR amplification reactions were performed for matrixes isolated from cultures of reference strains giving one predicted product for each sample. First attempts of T-DNA detection in infected soils and plants were performed. We hope that the presented new PCR system for Agrobacterium tumefaciens detection will help to fight the crown gall disease in the nearest future.", "gemini": "Thiết kế hệ thống PCR được trình bày trong công trình này dựa trên kiến thức về đặc tính phân tử của bệnh u sùi do vi khuẩn. Tính độc lực của Agrobacterium tumefaciens đòi hỏi phải có một plasmid Ti lớn (lên đến 235.000 bp) (pTi-plasmid gây u). Plasmid này mang đoạn T-DNA (T-DNA-DNA được chuyển), đoạn này tích hợp vào nhiễm sắc thể tế bào của cây bị nhiễm và sau đó làm thay đổi hình thái và quá trình trao đổi chất của cây. Không thể loại trừ rằng sau khi T-DNA tích hợp thì sự hiện diện của Agrobacterium không cần thiết cho sự phát triển của các thay đổi bệnh lý. Do đó, T-DNA là dấu hiệu duy nhất phải có mặt ở cả vi khuẩn độc lực và cây bị nhiễm ở bất kỳ giai đoạn nào của bệnh và thậm chí trước khi nhiễm trùng. Đây là lý do tại sao T-DNA được chọn làm vùng đích để khuếch đại PCR. Các đoạn mồi kẹp một đoạn 220 bp của một trong những vùng bảo tồn chịu trách nhiệm về độc lực của Agrobacterium, cụ thể là gen tms2 mã hóa cho indolacetamide amidohydrolase (bước thứ hai của quá trình sinh tổng hợp auxin) được thiết kế là tối ưu cho việc khuếch đại PCR. Các phản ứng khuếch đại PCR được thực hiện đối với các ma trận được phân lập từ các chủng tham chiếu, cho một sản phẩm dự đoán cho mỗi mẫu. Những nỗ lực đầu tiên để phát hiện T-DNA trong đất và cây bị nhiễm đã được thực hiện. Chúng tôi hy vọng rằng hệ thống PCR mới được trình bày để phát hiện Agrobacterium tumefaciens sẽ giúp chống lại bệnh u sùi trong tương lai gần."} +{"idx": 1940135, "en": "Various genera of sulphate reducing bacteria (SRB) have been found in the human digestive tract. It is suggested that some of SRB species may be responsible for the development of the clinical symptoms of ulcerative colitis and other disease of large intestine. Sulphasalazine (salicyl-azo-sulphapyridine, SAS) is commonly used to treat patients with ulcerative colitis and Crohn disease. Above 30 samples of faeces or biopsy specimens from 25 patients (age 45 +/- 14 years; M/F, 13/12) suffering from gastrointestinal disorders were used for isolation of Desulfovibrio desulfuricans species. The morphological, physiological and biochemical characteristics of isolated strains and also their susceptibility to SAS was determined. D. desulfuricans isolates were obtained from 5 amongst all patients assayed. Some abnormal, cigar-shaped cells were detected as accompanying the cells represented by rods, curved rods and vibrios. After strains purification, two types of colonies were present on the solid Postgate's medium B (containing lactate as a carbon source and sulphate for energy conservation): the black colonies growing in bulk of agar medium and the transparent, surface-growing mucous colonies. These two types of D. desulfuricans colonies may be a result of different iron availability for bacterial cells. High metabolic activity of strain was not always accompanied by the presence of H2S gas lock in the test tube, although the H2S odor was perceptible. All tested strains multiplied inconsiderably slowly in the presence of SAS at concentrations 10, 20, 40 and 60 mg/cm3. The growing concentrations of SAS did not cause a proportional decrease of the bacterial cells number. Taking into account the positive results of using SAS to treat patients with some colonic diseases and the indicated resistance to SAS of intestinal D. desulfuricans strains, it appears probable, that this SRB species isn't responsible for the development of mentioned diseases.", "gemini": "Nhiều chi vi khuẩn khử sulfat (SRB) đã được tìm thấy trong đường tiêu hóa của con người. Người ta cho rằng một số loài SRB có thể chịu trách nhiệm cho sự phát triển các triệu chứng lâm sàng của bệnh viêm loét đại tràng và các bệnh khác của ruột già. Sulphasalazine (salicyl-azo-sulphapyridine, SAS) thường được sử dụng để điều trị bệnh nhân bị viêm loét đại tràng và bệnh Crohn. Trên 30 mẫu phân hoặc mẫu sinh thiết từ 25 bệnh nhân (tuổi 45 +/- 14 năm; Nam/Nữ, 13/12) bị rối loạn tiêu hóa đã được sử dụng để phân lập các loài Desulfovibrio desulfuricans. Các đặc điểm về hình thái, sinh lý và sinh hóa của các chủng đã phân lập và cả tính nhạy cảm của chúng với SAS đã được xác định. Các phân lập D. desulfuricans đã thu được từ 5 trong số tất cả các bệnh nhân được xét nghiệm. Một số tế bào bất thường hình điếu xì gà đã được phát hiện đi kèm với các tế bào được biểu diễn bằng các thanh, thanh cong và phẩy khuẩn. Sau khi làm tinh khiết các chủng, có hai loại khuẩn lạc xuất hiện trên môi trường rắn Postgate B (chứa lactat làm nguồn cacbon và sulfat để bảo tồn năng lượng): các khuẩn lạc màu đen phát triển ở dạng khối trong môi trường thạch và các khuẩn lạc nhầy trong suốt phát triển trên bề mặt. Hai loại khuẩn lạc D. desulfuricans này có thể là kết quả của sự sẵn có sắt khác nhau cho các tế bào vi khuẩn. Hoạt động trao đổi chất cao của chủng không phải lúc nào cũng đi kèm với sự hiện diện của khóa khí H2S trong ống nghiệm, mặc dù mùi H2S có thể nhận thấy được. Tất cả các chủng được thử nghiệm đều nhân lên chậm đáng kể khi có SAS ở nồng độ 10, 20, 40 và 60 mg/cm3. Nồng độ SAS tăng không gây ra sự giảm tỷ lệ thuận của số lượng tế bào vi khuẩn. Có tính đến các kết quả khả quan của việc sử dụng SAS để điều trị bệnh nhân mắc một số bệnh đại tràng và khả năng kháng SAS của các chủng D. desulfuricans đường ruột, có vẻ có khả năng là loài SRB này không chịu trách nhiệm cho sự phát triển của các bệnh đã đề cập."} +{"idx": 1940136, "en": "Cell walls in the mycelium of Aspergillus niger strains sensitive or resistant to toxic molasses compounds growing in the presence of Spumol K were the object of the present studies. Although the inhibitory effect of Spumol K on the cell wall was noticed in studied mycelia the sensitive strains reacted more strongly. In sensitive strains in Spumol K presence lower content of cell wall was accomplished by higher amount of proteins but lower of lipids as well as structural polymers i.e. of glucans and chitin in this wall. A weaker synthesis of chitin and lower level of cell wall enzymes, especially chitinase, were observed in these strains. The described changes seem to be responsible for the growth inhibition and mycelium sinking in sensitive strains of A. niger.", "gemini": "Thành tế bào trong sợi nấm của các chủng Aspergillus niger nhạy cảm hoặc kháng với các hợp chất mật đường độc hại phát triển khi có mặt Spumol K là đối tượng của các nghiên cứu hiện tại. Mặc dù tác dụng ức chế của Spumol K lên thành tế bào đã được ghi nhận ở các sợi nấm được nghiên cứu, các chủng nhạy cảm phản ứng mạnh hơn. Ở các chủng nhạy cảm có sự hiện diện của Spumol K, hàm lượng thành tế bào thấp hơn đạt được với lượng protein cao hơn nhưng lượng lipid cũng như các polyme cấu trúc tức là glucan và chitin trong thành tế bào này thấp hơn. Sự tổng hợp chitin yếu hơn và mức thấp hơn của các enzym thành tế bào, đặc biệt là chitinase, đã được quan sát thấy ở các chủng này. Những thay đổi được mô tả dường như chịu trách nhiệm cho sự ức chế tăng trưởng và sợi nấm chìm ở các chủng nhạy cảm của A. niger."} +{"idx": 1940137, "en": "beta-glucosidase (EC 3.2.1.21) was isolated from the culture filtrate of Aspergillus niger IBT-90. The crude extracellular enzyme preparation was fractionated by six step purification procedure, (NH4)2SO4 precipitation, gel filtration on Bio-Gel P-10 and P-100, an ion-exchange chromatography on DEAE Bio-Gel A, yielding beta-glucosidase with an isoelectric point at pH 4.05. The enzyme was found to be a dimer with an apparent molecular weight of approximately 200 kDa as determined by size exclusion chromatography. It is composed of two apparently identical subunits of about 100 kDa (determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis). A. niger IBT-90 beta-glucosidase contains 33% carbohydrates. It is most active towards cellobiose at pH 4.8 and 65 degrees C. The enzyme sequentially splits off glucose units from non reducing ends of collodextrins. Kinetic studies on cellobiose and salicin hydrolysis, in concentration from 0.1 to 5.0 mM, resulted in non-linear Lineweaver-Burk and Hanes plots, whereas p-nitrophenyl-beta-D-glucopiranoside (pNPG) did not induce this type of effect. No metal ion is required for the enzyme catalytic activity. Hg2+ and N-bromosuccinimide (NBS) are its strong inhibitors. Glucono-delta-lactone and glucose are competitive inhibitors of the enzyme and glucono-delta-lactone is more potent of the two.", "gemini": "beta-glucosidase (EC 3.2.1.21) được phân lập từ dịch lọc nuôi cấy của Aspergillus niger IBT-90. Chế phẩm enzym ngoại bào thô được phân đoạn bằng quy trình tinh chế sáu bước, kết tủa (NH4)2SO4, lọc gel trên Bio-Gel P-10 và P-100, sắc ký trao đổi ion trên DEAE Bio-Gel A, tạo ra beta-glucosidase với điểm đẳng điện ở pH 4,05. Người ta thấy rằng enzym là một chất dimer có trọng lượng phân tử biểu kiến khoảng 200 kDa được xác định bằng sắc ký loại trừ kích thước. Nó bao gồm hai tiểu đơn vị dường như giống hệt nhau có trọng lượng khoảng 100 kDa (xác định bằng điện di gel polyacrylamide natri dodecyl sulfat). A. niger IBT-90 beta-glucosidase chứa 33% carbohydrate. Nó hoạt động mạnh nhất đối với cellobiose ở pH 4,8 và 65 độ C. Enzym tuần tự phân tách các đơn vị glucose ra khỏi các đầu không khử của collodextrin. Các nghiên cứu động học về quá trình thủy phân cellobiose và salicin, ở nồng độ từ 0,1 đến 5,0 mM, dẫn đến các đồ thị Lineweaver-Burk và Hanes phi tuyến tính, trong khi p-nitrophenyl-beta-D-glucopiranoside (pNPG) không gây ra loại hiệu ứng này. Không cần ion kim loại nào cho hoạt động xúc tác của enzym. Hg2+ và N-bromosuccinimide (NBS) là các chất ức chế mạnh của nó. Glucono-delta-lactone và glucose là các chất ức chế cạnh tranh của enzym và glucono-delta-lactone mạnh hơn trong hai chất này."} +{"idx": 1940138, "en": "Staphylococcal infections constitute one of the main problems associated with clinical applications of various prosthetic medical devices (biomaterials). As the magnitude of the infection risk depends often on the duration of device installation, and the incidence of infections is higher in skin-penetrating devices, we studied some parameters of specific immune response to staphylococcal antigens in mice subcutaneously (s.c.) implanted for three months with heparinized polyethylene (H-PE). Three weeks before the evaluation of immune response, mice (implanted and non-implanted) were s.c. infected with 10(7) of Staphylococcus aureus Cowan 1. The proliferation of lymph node cells was determined on the basis of 3H-thymidine incorporation in 3-days cultures stimulated with: staphylococcal lipoteichoic acid (LTA), protein A (SpA), alpha-toxin, or with phytohemagglutinin (PHA). Moreover, the levels of specific antibodies to staphylococcal antigens were determined in serum samples (ELISA against: LTA, SpA, alpha-toxin). The data obtained indicate that long-lasting implantation caused evident changes in proliferative activity of lymphocytes and humoral response to staphylococcal antigens. It enhances alpha-toxin and LTA stimulated proliferation of lymph node lymphocytes in vitro. In contrast, H-PE-implanted animals demonstrated a significant decrease in the production of anti-SpA IgG2a and IgG2b and increase in the synthesis of anti-LTA IgG1 antibodies.", "gemini": "Nhiễm trùng tụ cầu là một trong những vấn đề chính liên quan đến các ứng dụng lâm sàng của nhiều thiết bị y tế giả (vật liệu sinh học). Vì mức độ nguy cơ nhiễm trùng thường phụ thuộc vào thời gian lắp đặt thiết bị và tỷ lệ nhiễm trùng cao hơn ở các thiết bị xuyên da, chúng tôi đã nghiên cứu một số thông số về phản ứng miễn dịch đặc hiệu đối với kháng nguyên tụ cầu ở chuột được cấy dưới da (s.c.) trong ba tháng bằng polyethylene heparin hóa (H-PE). Ba tuần trước khi đánh giá phản ứng miễn dịch, chuột (đã cấy ghép và chưa cấy ghép) đã bị nhiễm s.c. với 10 (7) Staphylococcus aureus Cowan 1. Sự gia tăng của tế bào hạch bạch huyết được xác định trên cơ sở kết hợp 3H-thymidine trong nuôi cấy 3 ngày được kích thích bằng: axit lipoteichoic tụ cầu (LTA), protein A (SpA), alpha-toxin hoặc với phytohemagglutinin (PHA). Hơn nữa, nồng độ kháng thể đặc hiệu đối với kháng nguyên tụ cầu đã được xác định trong các mẫu huyết thanh (ELISA chống lại: LTA, SpA, alpha-toxin). Dữ liệu thu được chỉ ra rằng việc cấy ghép lâu dài gây ra những thay đổi rõ ràng trong hoạt động tăng sinh của tế bào lympho và phản ứng dịch thể đối với kháng nguyên tụ cầu. Nó làm tăng sự gia tăng alpha-toxin và LTA kích thích sự gia tăng của tế bào lympho hạch bạch huyết trong ống nghiệm. Ngược lại, động vật cấy ghép H-PE cho thấy sự giảm đáng kể trong sản xuất kháng thể IgG2a và IgG2b chống SpA và tăng tổng hợp kháng thể IgG1 chống LTA."} +{"idx": 1940139, "en": "New glycoprotein (CML) isolated from Chelidonium maius exhibits good antibacterial activity against methicillin resistant staphylococci and enterococci. It may constitute new antimicrobial agent against methicillin and vancomycin-resistant staphylococci as well as multiresistant enterococci.", "gemini": "Glycoprotein mới (CML) được phân lập từ Chelidonium maius cho thấy hoạt tính kháng khuẩn tốt đối với tụ cầu và enterococci kháng methicillin. Nó có thể cấu thành tác nhân chống vi khuẩn mới chống lại tụ cầu kháng methicillin và vancomycin cũng như enterococci đa kháng."} +{"idx": 1940140, "en": "In the enantioselective esterification of racemic naproxen with 4-(2-hydroxyethyl) morpholine by Lipase MY in organic solvents, a productivity improvement of the desired (S)-naproxen ester from 0.42 to 0.72 mM at the reaction time of 130 h was observed, when the surfactant bis (2-ethylhexyl) sodium sulfosuccinate (AOT) was added in the reaction mixture. The presence of a small amount of exogenously added water dramatically activated the enzyme in AOT/cyclohexane-reversed micelles. Desorption of the surfactant molecule from the enzyme mass and solubilization of the enzyme into reversed micelles were used to elucidate an existing maximum of the initial rate of (S)-naproxen synthesis with the water content. Moreover, the effects of alcohol and surfactant concentration on the enzyme activity are reported.", "gemini": "Trong quá trình este hóa chọn lọc quang học của naproxen racemic với 4- (2-hydroxyethyl) morpholine bằng Lipase MY trong dung môi hữu cơ, năng suất cải thiện của este (S) -naproxen mong muốn từ 0,42 đến 0,72 mM tại thời điểm phản ứng 130 h được quan sát thấy khi chất hoạt động bề mặt bis (2-ethylhexyl) natri sulfosuccinat (AOT) được thêm vào hỗn hợp phản ứng. Sự hiện diện của một lượng nhỏ nước được thêm vào bên ngoài đã kích hoạt mạnh mẽ enzyme trong micelle đảo ngược AOT / cyclohexane. Sự giải hấp phụ của phân tử chất hoạt động bề mặt từ khối lượng enzyme và sự hòa tan của enzyme vào micelle đảo ngược đã được sử dụng để làm sáng tỏ mức tối đa hiện có của tốc độ tổng hợp (S) -naproxen ban đầu với hàm lượng nước. Hơn nữa, các tác động của nồng độ rượu và chất hoạt động bề mặt đối với hoạt động của enzyme được báo cáo."} +{"idx": 1940141, "en": "Membrane fragments or membrane proteins within a lipid mixture were immobilized over metal electrodes. This procedure has been developed to study biological membranes without interference from cell machinery. To obtain a smooth hydrophilic biomembrane support and a mode of binding of the membrane, either a crosslinked gel or an aromatic polyamine-polymer doped with avidin was deposited at the metal electrode by electropolymerization. This layer (less than 10 nm thick) also served as a submembrane compartment. The facilitated glucose transporter (GLUT-1) purified from human erythrocytes was integrated into a lipid membrane containing artificial biotinylated lipids and reacted with the activated surface of the glucose sensitive electrode. It was demonstrated that the lipid layer was attached to the polymer-containing avidin and could only be removed by detergent extraction. The presence of an active membrane transporter was demonstrated by electrochemical detection of glucose in the submembrane compartment, and by inhibition of glucose transport with the specific inhibitor Cytochalasin-B.", "gemini": "Các mảnh màng hoặc protein màng trong hỗn hợp lipid được cố định trên các điện cực kim loại. Quy trình này đã được phát triển để nghiên cứu màng sinh học mà không bị nhiễu từ máy móc tế bào. Để có được một giá đỡ màng sinh học ưa nước mịn và một phương thức liên kết màng, một gel liên kết chéo hoặc một polyamine-polyme thơm được bổ sung avidin đã được lắng đọng tại điện cực kim loại bằng phương pháp điện trùng hợp. Lớp này (dày dưới 10 nm) cũng đóng vai trò như một khoang màng phụ. Chất vận chuyển glucose được tạo thuận lợi (GLUT-1) được tinh chế từ hồng cầu người đã được tích hợp vào màng lipid có chứa lipid biotin hóa nhân tạo và phản ứng với bề mặt hoạt động của điện cực nhạy glucose. Người ta đã chứng minh rằng lớp lipid được gắn vào avidin chứa polyme và chỉ có thể loại bỏ bằng cách chiết xuất chất tẩy rửa. Sự hiện diện của một chất vận chuyển màng hoạt động đã được chứng minh bằng cách phát hiện glucose điện hóa trong khoang màng phụ và bằng cách ức chế vận chuyển glucose với chất ức chế đặc hiệu Cytochalasin-B."} +{"idx": 1940142, "en": "In an attempt to produce the polyunsaturated fatty acid (PUFA)-enriched glycerides, commercially available Turkish anchovy oil (PUFA content of 27%), was hydrolyzed with 1,3-specific Rhizomucor miehei lipase. After the hydrolysis, the triglyceride (TG), diglyceride (DG), monoglyceride (MG), and free fatty acid (FFA) composition of the reaction mixture was determined, and fatty acid components of these fractions were analyzed. R. miehei lipase released PUFA extremely slowly, resulting in their accumulation in the TG and DG fractions, especially in TG. The PUFA content in the glyceride mixture (including TG, DG, and MG) increased as hydrolysis progressed. The effects of operational parameters (pH, temperature, time, and enzyme concentration) on the extent of hydrolysis were investigated. Based on these results, optimal reaction conditions were established. At optimal conditions (pH 4.0, 35 degrees C, 3 h, and enzyme concentration of 500 U/g oil), the level of PUFA in the glyceride mixture was raised to 40%. The individual TG and DG fractions contained 45 and 30% PUFA, respectively. Less than 2% of the total PUFA was lost in the FFA fraction.", "gemini": "Trong nỗ lực sản xuất các glycerid làm giàu axit béo không bão hòa đa (PUFA), dầu cá cơm Thổ Nhĩ Kỳ có sẵn trên thị trường (hàm lượng PUFA là 27%) đã được thủy phân bằng lipase Rhizomucor miehei đặc hiệu 1,3. Sau khi thủy phân, thành phần triglyceride (TG), diglyceride (DG), monoglyceride (MG) và axit béo tự do (FFA) của hỗn hợp phản ứng được xác định và các thành phần axit béo của các phân đoạn này được phân tích. Lipase R. miehei giải phóng PUFA cực kỳ chậm, dẫn đến sự tích tụ của chúng trong các phân đoạn TG và DG, đặc biệt là ở TG. Hàm lượng PUFA trong hỗn hợp glycerid (bao gồm TG, DG và MG) tăng lên khi quá trình thủy phân diễn ra. Các tác động của các thông số vận hành (pH, nhiệt độ, thời gian và nồng độ enzyme) đối với mức độ thủy phân đã được nghiên cứu. Dựa trên những kết quả này, các điều kiện phản ứng tối ưu đã được thiết lập. Ở các điều kiện tối ưu (pH 4,0, 35 độ C, 3 giờ và nồng độ enzyme là 500 U/g dầu), mức PUFA trong hỗn hợp glycerid được nâng lên 40%. Các phân đoạn TG và DG riêng lẻ chứa 45 và 30% PUFA. Ít hơn 2% tổng lượng PUFA bị mất trong phân đoạn FFA."} +{"idx": 1940143, "en": "Further characterization of an aspartyl protease from Mucor bacilliformis with milk-clotting activity was performed. An extinction coefficient, epsilon 278 cm = 1.61 mL/mg/cm, a molecular mass of 35,400 Da and a pI of 5.2 were determined. Proteolytic activity and kinetic parameters were evaluated by using the hexapeptide Leu-Ser-pNO2-Phe-Nle-Ala-Leu-OMe as the substrate. The effect of pH and temperature on peptide cleavage, as well as protease heat stability, was determined. Such properties, taken as a whole, indicate that the M. bacilliformis protease can be considered a potential substitute for bovine chymosin in cheese manufacture.", "gemini": "Đặc điểm tiếp theo của một aspartyl protease từ Mucor bacilliformis có hoạt tính đông sữa đã được thực hiện. Hệ số hấp thụ, epsilon 278 cm = 1,61 mL/mg/cm, khối lượng phân tử là 35.400 Da và pI là 5,2 đã được xác định. Hoạt tính proteolytic và các thông số động học đã được đánh giá bằng cách sử dụng hexapeptide Leu-Ser-pNO2-Phe-Nle-Ala-Leu-OMe làm chất nền. Tác động của pH và nhiệt độ đối với quá trình phân cắt peptide, cũng như độ ổn định nhiệt của protease, đã được xác định. Những tính chất như vậy, được coi là một tổng thể, chỉ ra rằng protease M. bacilliformis có thể được coi là chất thay thế tiềm năng cho chymosin ở bò trong sản xuất phô mai."} diff --git a/retranslated_vi_pubmed/chunk_194/error.json b/retranslated_vi_pubmed/chunk_194/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_194/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_195/completed.json b/retranslated_vi_pubmed/chunk_195/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..8ba7e0be639dc3dc5faeaccca6226de7d61f9191 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_195/completed.json @@ -0,0 +1,146 @@ +[ + 1950000, + 1950001, + 1950002, + 1950003, + 1950004, + 1950005, + 1950006, + 1950007, + 1950008, + 1950009, + 1950010, + 1950011, + 1950012, + 1950013, + 1950014, + 1950015, + 1950016, + 1950017, + 1950018, + 1950019, + 1950020, + 1950021, + 1950022, + 1950023, + 1950024, + 1950025, + 1950026, + 1950027, + 1950028, + 1950029, + 1950030, + 1950031, + 1950032, + 1950033, + 1950034, + 1950035, + 1950036, + 1950037, + 1950038, + 1950039, + 1950040, + 1950041, + 1950042, + 1950043, + 1950044, + 1950045, + 1950046, + 1950047, + 1950048, + 1950049, + 1950050, + 1950051, + 1950052, + 1950053, + 1950054, + 1950055, + 1950056, + 1950057, + 1950058, + 1950059, + 1950060, + 1950061, + 1950062, + 1950063, + 1950064, + 1950065, + 1950066, + 1950067, + 1950068, + 1950069, + 1950070, + 1950071, + 1950072, + 1950073, + 1950074, + 1950075, + 1950076, + 1950077, + 1950078, + 1950079, + 1950080, + 1950081, + 1950082, + 1950083, + 1950084, + 1950085, + 1950086, + 1950087, + 1950088, + 1950089, + 1950090, + 1950091, + 1950092, + 1950093, + 1950094, + 1950095, + 1950096, + 1950097, + 1950098, + 1950099, + 1950100, + 1950101, + 1950102, + 1950103, + 1950104, + 1950105, + 1950106, + 1950107, + 1950108, + 1950109, + 1950110, + 1950111, + 1950112, + 1950113, + 1950114, + 1950115, + 1950116, + 1950117, + 1950118, + 1950119, + 1950120, + 1950121, + 1950122, + 1950123, + 1950124, + 1950125, + 1950126, + 1950127, + 1950128, + 1950129, + 1950130, + 1950131, + 1950132, + 1950133, + 1950134, + 1950135, + 1950136, + 1950137, + 1950138, + 1950139, + 1950140, + 1950141, + 1950142, + 1950143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_195/data.jsonl b/retranslated_vi_pubmed/chunk_195/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..840fcf56abf956ad18547ec314004996cbdade2d --- /dev/null +++ b/retranslated_vi_pubmed/chunk_195/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1950000, "en": "OBJECTIVE To study insulin-like factor-I (IGF-I) levels in children and adolescents with connective tissue diseases (CTDs), compare them with values obtained in normal controls, and correlate them with age, sex, steroid treatment, and inflammatory parameters. METHODS A multicenter, cross-sectional study was performed in 3 Italian pediatric rheumatology centers. The subjects studied comprised 117 patients with juvenile arthritis (53 systemic, 25 pauciarticular and 17 polyarticular) and other CTDs (22), and 78 children without inflammatory conditions. IGF-I levels were measured by radioimmunoassay after acid-ethanol extraction. RESULTS Mean IGF-I serum levels were 167.6 ng/ml (+/- 132.5) in patients and 214.4 (+/- 142.8) in controls. A significant correlation was found between IGF-I levels and age in the controls (P = 0.001), but not in the patients. Covariance analysis with age as the covariate showed significantly lower IGF-I levels in the patient group (P = 0.001). No significant correlation was found between IGF-I levels and the total quantity of steroid taken. Multiple regression analysis showed that IGF-I levels were inversely correlated with the ESR (P = 0.0001) and positively correlated with age (P = 0.0002) and sex (P = 0.021) in the patient group. CONCLUSION IGF-I serum levels are decreased in patients with CTDs; inflammation could play a major role.", "gemini": "MỤC TIÊU Nghiên cứu nồng độ yếu tố giống insulin-I (IGF-I) ở trẻ em và thanh thiếu niên mắc bệnh mô liên kết (CTD), so sánh với các giá trị thu được ở nhóm đối chứng bình thường và tương quan với tuổi, giới tính, điều trị steroid và các thông số viêm. PHƯƠNG PHÁP Một nghiên cứu cắt ngang, đa trung tâm đã được thực hiện tại 3 trung tâm thấp khớp nhi khoa của Ý. Đối tượng nghiên cứu gồm 117 bệnh nhân mắc bệnh viêm khớp trẻ em (53 toàn thân, 25 ít khớp và 17 nhiều khớp) và các bệnh CTD khác (22) và 78 trẻ không có tình trạng viêm. Nồng độ IGF-I được đo bằng phương pháp miễn dịch phóng xạ sau khi chiết xuất axit-ethanol. KẾT QUẢ Nồng độ IGF-I huyết thanh trung bình là 167,6 ng/ml (+/- 132,5) ở bệnh nhân và 214,4 (+/- 142,8) ở nhóm đối chứng. Tương quan đáng kể được tìm thấy giữa nồng độ IGF-I và tuổi ở nhóm đối chứng (P = 0,001), nhưng không phải ở nhóm bệnh nhân. Phân tích hiệp phương sai với tuổi là biến hiệp phương sai cho thấy nồng độ IGF-I thấp hơn đáng kể ở nhóm bệnh nhân (P = 0,001). Không tìm thấy mối tương quan đáng kể nào giữa nồng độ IGF-I và tổng lượng steroid đã dùng. Phân tích hồi quy đa biến cho thấy nồng độ IGF-I tương quan nghịch với ESR (P = 0,0001) và tương quan thuận với tuổi (P = 0,0002) và giới tính (P = 0,021) ở nhóm bệnh nhân. KẾT LUẬN Nồng độ IGF-I huyết thanh giảm ở những bệnh nhân mắc bệnh CTD; tình trạng viêm có thể đóng vai trò chính."} +{"idx": 1950001, "en": "Although increased risk for autoimmune diseases has been documented in Turner's syndrome (TS), the involvement of juvenile rheumatoid arthritis (JRA) has rarely been reported. A detailed case description of JRA associated with Turner's syndrome is presented as the second such case report in the literature. The arthritis was diagnosed 8 years after its onset due to the confounding of its symptoms with those of TS.", "gemini": "Mặc dù nguy cơ mắc các bệnh tự miễn đã được ghi nhận trong hội chứng Turner (TS), nhưng tình trạng viêm khớp dạng thấp ở trẻ em (JRA) hiếm khi được báo cáo. Một mô tả trường hợp chi tiết về JRA liên quan đến hội chứng Turner được trình bày như báo cáo trường hợp thứ hai như vậy trong tài liệu. Viêm khớp được chẩn đoán sau 8 năm khởi phát do các triệu chứng của nó bị nhầm lẫn với các triệu chứng của TS."} +{"idx": 1950002, "en": "Pulmonary hemorrhage (PH) is a rare but potentially life-threatening manifestation of systemic lupus erythematosus (SLE). In this report we describe a 13 year old girl with PH as the sole presenting clinical manifestation of her SLE. Her serology was diagnostic of SLE and one year after presentation she developed arthritis. She had a rapid serologic but delayed clinical response to combination therapy of intravenous pulse methylprednisolone, pulse cyclophosphamide and daily prednisone. Awareness of the possibility of pulmonary hemorrhage as a presentation of SLE may aid in the diagnosis and early, aggressive management of this condition.", "gemini": "Xuất huyết phổi (PH) là một biểu hiện hiếm gặp nhưng có khả năng đe dọa tính mạng của bệnh lupus ban đỏ hệ thống (SLE). Trong báo cáo này, chúng tôi mô tả một bé gái 13 tuổi bị PH là biểu hiện lâm sàng duy nhất khi mắc SLE. Huyết thanh học của cô bé chẩn đoán là SLE và một năm sau khi biểu hiện, cô bé phát triển viêm khớp. Cô bé có đáp ứng huyết thanh học nhanh nhưng đáp ứng lâm sàng chậm với liệu pháp kết hợp methylprednisolone xung tĩnh mạch, cyclophosphamide xung và prednisone hàng ngày. Nhận thức về khả năng xuất huyết phổi như một biểu hiện của SLE có thể hỗ trợ chẩn đoán và quản lý tích cực, sớm tình trạng này."} +{"idx": 1950003, "en": "The purpose of this study was to review the results of trials assessing the association between the use of calcium channel blockers (CCBs) and mortality, myocardial infarction (MI), and cancer. Possible mechanisms of such relationships are discussed and recommendations regarding the use of CCBs made. Since 1995, 10 controversial studies have been published that associate the use of CCBs with an increased risk of mortality, MI, and cancer; these findings have caused widespread anxiety and frustration among patients and physicians. For health care professionals to properly advise patients, the facts surrounding this controversy should be reviewed. To do this, we reviewed and assessed English-language clinical studies, abstracts, editorials, and review articles pertaining to the use of CCBs and mortality, MI, and cancer in humans. The designs of ongoing prospective, randomized studies are discussed. Based on current published studies, the US Food and Drug Administration has agreed to a label warning against off-label use of short-acting nifedipine in patients with hypertension, acute MI, or nonvasospastic unstable angina. Practitioners should exercise caution when prescribing CCBs, especially to high-risk patients (e.g., those with congestive heart failure or clinical or subclinical coronary artery disease). When possible, long-acting CCBs should be used.", "gemini": "Mục đích của nghiên cứu này là xem xét kết quả của các thử nghiệm đánh giá mối liên quan giữa việc sử dụng thuốc chẹn kênh canxi (CCB) với tỷ lệ tử vong, nhồi máu cơ tim (MI) và ung thư. Các cơ chế có thể xảy ra của những mối quan hệ như vậy được thảo luận và đưa ra các khuyến nghị liên quan đến việc sử dụng CCB. Kể từ năm 1995, 10 nghiên cứu gây tranh cãi đã được công bố liên quan đến việc sử dụng CCB với nguy cơ tử vong, MI và ung thư tăng lên; những phát hiện này đã gây ra sự lo lắng và thất vọng trên diện rộng trong số bệnh nhân và bác sĩ. Để các chuyên gia chăm sóc sức khỏe tư vấn đúng cho bệnh nhân, cần xem xét các sự kiện xung quanh cuộc tranh cãi này. Để làm được điều này, chúng tôi đã xem xét và đánh giá các nghiên cứu lâm sàng, tóm tắt, bài xã luận và bài báo tổng quan bằng tiếng Anh liên quan đến việc sử dụng CCB và tỷ lệ tử vong, MI và ung thư ở người. Các thiết kế của các nghiên cứu ngẫu nhiên, có triển vọng đang được thảo luận. Dựa trên các nghiên cứu đã công bố hiện tại, Cục Quản lý Thực phẩm và Dược phẩm Hoa Kỳ đã đồng ý dán nhãn cảnh báo chống lại việc sử dụng nifedipine tác dụng ngắn không đúng nhãn ở những bệnh nhân bị tăng huyết áp, nhồi máu cơ tim cấp hoặc đau thắt ngực không co thắt. Các bác sĩ nên thận trọng khi kê đơn CCB, đặc biệt là đối với những bệnh nhân có nguy cơ cao (ví dụ: những người bị suy tim sung huyết hoặc bệnh động mạch vành lâm sàng hoặc cận lâm sàng). Khi có thể, nên sử dụng CCB tác dụng dài."} +{"idx": 1950004, "en": "The incidence of benign prostatic hyperplasia (BPH) is increasing, and an estimated one quarter of men older than 60 years have both BPH and hypertension. Medical management of BPH is now an effective alternative to surgery, and primary care physicians are treating more patients for this condition. Alpha-adrenergic blockers reduce the tone of prostatic smooth muscle, and doxazosin, a selective alpha-1 adrenergic blocker, is effective and well tolerated in the treatment of BPH. Doxazosin can be given in a convenient once-daily dose, and its efficacy is maintained in the long term. Patients with BPH who are already being treated for hypertension with doxazosin need no alteration in their dosage regimen, and doxazosin can be used as a single agent to treat both conditions. Selective alpha-adrenergic blockers are likely to be used increasingly by primary care physicians in the treatment of BPH.", "gemini": "Tỷ lệ tăng sản lành tính tuyến tiền liệt (BPH) đang gia tăng và ước tính có khoảng một phần tư nam giới trên 60 tuổi mắc cả BPH và tăng huyết áp. Quản lý y tế đối với BPH hiện là một phương pháp thay thế hiệu quả cho phẫu thuật và các bác sĩ chăm sóc chính đang điều trị nhiều bệnh nhân hơn cho tình trạng này. Thuốc chẹn alpha-adrenergic làm giảm trương lực cơ trơn tuyến tiền liệt và doxazosin, một thuốc chẹn alpha-1 adrenergic chọn lọc, có hiệu quả và được dung nạp tốt trong điều trị BPH. Doxazosin có thể được dùng với liều một lần mỗi ngày rất tiện lợi và hiệu quả của thuốc được duy trì trong thời gian dài. Những bệnh nhân bị BPH đã được điều trị tăng huyết áp bằng doxazosin không cần thay đổi chế độ dùng thuốc và doxazosin có thể được sử dụng như một tác nhân duy nhất để điều trị cả hai tình trạng. Thuốc chẹn alpha-adrenergic chọn lọc có khả năng sẽ được các bác sĩ chăm sóc chính sử dụng ngày càng nhiều trong điều trị BPH."} +{"idx": 1950005, "en": "Paget's disease of bone is a common disorder of unknown etiology characterized by increased bone remodeling and abnormal bone architecture. The pathologic process is initiated by an increase in osteoclast-mediated bone resorption, accompanied by a compensatory increase in bone formation. The increased bone remodeling results in a disorganized mosaic of woven and lamellar bone. This bone is highly vascular and gradually becomes enlarged and structurally weakened. Paget's disease is generally diagnosed in patients older than 40 years of age, usually as an incidental finding. The disease may be monostotic or polyostotic. The pelvis, femur, spine, tibia, skull, and humerus are most commonly involved. Most patients with Paget's disease are asymptomatic. Pain is the most common presenting symptom. Complications of the disease include bowing deformity of the long bones, fracture, and a variety of nerve compression syndromes. Malignant degeneration of Paget's disease is a rare complication. As safer, more effective therapies have become available, the indications for treatment and goals of therapy have changed. The difficult issue that clinicians are currently facing is whether to treat patients with asymptomatic disease. The progressive nature of the disease, the severity of its complications, its potential negative impact on quality of life, and the availability of therapy capable of controlling its activity have led many experts in the field to recommend treatment of asymptomatic patients who have active disease at sites where complications are likely to develop. There are, however, no data to prove that complications can be prevented by decreasing the rate of bone remodeling in Paget's disease, nor any data to define who is at risk for complications. Until more information is available, the management of patients with Paget's disease will continue to be based on clinical observation and theoretical considerations. This review examines the present understanding of Paget's disease, the rationale for the proposed indications for treatment and the goals of therapy.", "gemini": "Bệnh Paget xương là một rối loạn phổ biến có nguyên nhân chưa rõ, đặc trưng bởi sự tái tạo xương gia tăng và cấu trúc xương bất thường. Quá trình bệnh lý được khởi phát bởi sự gia tăng hủy xương do tế bào hủy xương, kèm theo sự gia tăng bù trừ trong quá trình hình thành xương. Sự tái tạo xương gia tăng dẫn đến một sự hỗn loạn của xương dệt và xương phiến. Loại xương này có mạch máu cao và dần dần trở nên to ra và yếu đi về mặt cấu trúc. Bệnh Paget thường được chẩn đoán ở những bệnh nhân trên 40 tuổi, thường là một phát hiện tình cờ. Bệnh có thể là đơn xương hoặc đa xương. Xương chậu, xương đùi, cột sống, xương chày, hộp sọ và xương cánh tay thường bị ảnh hưởng nhất. Hầu hết bệnh nhân mắc bệnh Paget không có triệu chứng. Đau là triệu chứng xuất hiện phổ biến nhất. Các biến chứng của bệnh bao gồm biến dạng cong của xương dài, gãy xương và nhiều hội chứng chèn ép thần kinh. Thoái hóa ác tính của bệnh Paget là một biến chứng hiếm gặp. Khi các phương pháp điều trị an toàn hơn, hiệu quả hơn đã có sẵn, các chỉ định điều trị và mục tiêu điều trị đã thay đổi. Vấn đề khó khăn mà các bác sĩ lâm sàng hiện đang phải đối mặt là có nên điều trị cho những bệnh nhân không có triệu chứng hay không. Tính chất tiến triển của bệnh, mức độ nghiêm trọng của các biến chứng, tác động tiêu cực tiềm tàng của nó đến chất lượng cuộc sống và sự sẵn có của liệu pháp có khả năng kiểm soát hoạt động của bệnh đã khiến nhiều chuyên gia trong lĩnh vực này khuyến nghị điều trị cho những bệnh nhân không có triệu chứng nhưng mắc bệnh ở những vị trí có khả năng phát triển biến chứng. Tuy nhiên, không có dữ liệu nào chứng minh rằng có thể ngăn ngừa các biến chứng bằng cách giảm tỷ lệ tái tạo xương trong bệnh Paget, cũng như không có dữ liệu nào xác định được ai có nguy cơ gặp biến chứng. Cho đến khi có thêm thông tin, việc quản lý bệnh nhân mắc bệnh Paget sẽ tiếp tục dựa trên quan sát lâm sàng và các cân nhắc về mặt lý thuyết. Bài tổng quan này xem xét hiểu biết hiện tại về bệnh Paget, cơ sở lý luận cho các chỉ định điều trị được đề xuất và các mục tiêu điều trị."} +{"idx": 1950006, "en": "Patients with seasonal or allergic bronchial asthma experience an immediate allergic response caused by allergen-specific immunoglobulin E-mediated histamine release. The release of histamine and other chemical mediators may trigger airway hyperresponsiveness and exaggerated bronchoconstriction, characteristic features of allergic bronchial asthma. Traditional antihistamines have demonstrated only moderate activity of short duration against this disease. In contrast, loratadine, a potent, nonsedating, histamine-1-receptor antagonist with activity in seasonal and perennial allergic rhinitis, has demonstrated effective control of asthma symptoms, improved pulmonary function, and long duration of action in patients with allergic bronchial asthma. This review summarizes preclinical evidence for the antiallergic activity of loratadine and the results of clinical studies with oral loratadine in patients with allergic bronchial asthma.", "gemini": "Bệnh nhân hen phế quản theo mùa hoặc hen phế quản do dị ứng có phản ứng dị ứng tức thì do giải phóng histamine do immunoglobulin E đặc hiệu với chất gây dị ứng. Sự giải phóng histamine và các chất trung gian hóa học khác có thể gây ra tình trạng tăng phản ứng của đường thở và co thắt phế quản quá mức, là những đặc điểm của hen phế quản do dị ứng. Các thuốc kháng histamin truyền thống chỉ có tác dụng vừa phải trong thời gian ngắn đối với bệnh này. Ngược lại, loratadine, một chất đối kháng thụ thể histamine-1 mạnh, không gây buồn ngủ, có hoạt tính trong viêm mũi dị ứng theo mùa và quanh năm, đã chứng minh hiệu quả kiểm soát các triệu chứng hen suyễn, cải thiện chức năng phổi và thời gian tác dụng kéo dài ở những bệnh nhân hen phế quản do dị ứng. Tổng quan này tóm tắt các bằng chứng tiền lâm sàng về hoạt tính chống dị ứng của loratadine và kết quả của các nghiên cứu lâm sàng với loratadine đường uống ở những bệnh nhân hen phế quản do dị ứng."} +{"idx": 1950007, "en": "To evaluate the efficacy and safety of human lymphoblastoid interferon treatment (interferon alfa) for patients with compensated cirrhosis caused by hepatitis C virus (HCV) infection, we randomly assigned 82 cirrhotic patients with chronic HCV infection (44 men, 38 women; mean age, 58.6 years) to two groups: 41 patients were treated with interferon alfa (480 million U over 6 months), and the other patients received no drug treatment. HCV RNA genotypes were determined by polymerase chain reaction (PCR) testing using type-specific primers. HCV RNA levels were measured by competitive PCR testing. No untreated patients eliminated HCV RNA from the serum or had a decrease in the level of alanine aminotransferase to normal during the observation period. Of the 34 patients who completed interferon alfa treatment, 6 (17.6%) who were considered complete responders eliminated HCV RNA from the serum by the end of treatment and sustained this elimination throughout a 6-month follow-up period. Complete responders constituted 6 (46.2%) of 13 patients with HCV RNA levels < or = 10(5) copies/50 microL, but none of the 21 patients with levels > 10(5) copies/50 microL were complete responders. Two (7.1%) of 28 patients with genotype 1b infection and 4 (66.7%) of 6 with genotype 2a were complete responders. Five patients withdrew because of interferon alfa-induced side effects (1 for thrombocytopenia, 3 for severe general malaise, and 1 for impotence), and 2 withdrew after being diagnosed with hepatocellular carcinoma. Hepatic failure did not occur in any treated patient in the present study. These findings indicate that interferon alfa treatment is useful for compensated cirrhosis caused by HCV infection if the HCV RNA levels are low and the infection is of genotype 2a.", "gemini": "Để đánh giá hiệu quả và độ an toàn của phương pháp điều trị bằng interferon lymphoblastoid ở người (interferon alfa) cho những bệnh nhân bị xơ gan mất bù do nhiễm virus viêm gan C (HCV), chúng tôi đã chỉ định ngẫu nhiên 82 bệnh nhân xơ gan bị nhiễm HCV mạn tính (44 nam, 38 nữ; độ tuổi trung bình 58,6 tuổi) thành hai nhóm: 41 bệnh nhân được điều trị bằng interferon alfa (480 triệu U trong 6 tháng) và những bệnh nhân khác không được điều trị bằng thuốc. Kiểu gen RNA HCV được xác định bằng xét nghiệm phản ứng chuỗi polymerase (PCR) sử dụng các mồi đặc hiệu loại. Nồng độ RNA HCV được đo bằng xét nghiệm PCR cạnh tranh. Không có bệnh nhân nào không được điều trị loại bỏ được RNA HCV khỏi huyết thanh hoặc có sự giảm nồng độ alanin aminotransferase xuống mức bình thường trong thời gian quan sát. Trong số 34 bệnh nhân hoàn thành quá trình điều trị bằng interferon alfa, có 6 bệnh nhân (17,6%) được coi là đáp ứng hoàn toàn đã loại bỏ được RNA HCV khỏi huyết thanh vào cuối thời gian điều trị và duy trì sự loại bỏ này trong suốt thời gian theo dõi 6 tháng. Những người đáp ứng hoàn toàn chiếm 6 (46,2%) trong số 13 bệnh nhân có nồng độ RNA HCV < hoặc = 10(5) bản sao/50 microL, nhưng không có bệnh nhân nào trong số 21 bệnh nhân có nồng độ > 10(5) bản sao/50 microL là người đáp ứng hoàn toàn. Hai (7,1%) trong số 28 bệnh nhân nhiễm kiểu gen 1b và 4 (66,7%) trong số 6 bệnh nhân nhiễm kiểu gen 2a là những người đáp ứng hoàn toàn. Năm bệnh nhân đã rút khỏi nghiên cứu vì tác dụng phụ do interferon alfa gây ra (1 bệnh nhân bị giảm tiểu cầu, 3 bệnh nhân bị suy nhược toàn thân nghiêm trọng và 1 bệnh nhân bị bất lực), và 2 bệnh nhân đã rút khỏi nghiên cứu sau khi được chẩn đoán mắc ung thư biểu mô tế bào gan. Suy gan không xảy ra ở bất kỳ bệnh nhân nào được điều trị trong nghiên cứu này. Những phát hiện này chỉ ra rằng điều trị bằng interferon alfa có hiệu quả đối với bệnh xơ gan mất bù do nhiễm HCV nếu nồng độ RNA HCV thấp và nhiễm trùng là kiểu gen 2a."} +{"idx": 1950008, "en": "Twenty patients with aplastic anemia underwent long-term administration (10 weeks) of recombinant human granulocyte colony-stimulating factor (rhG-CSF) in doses from 50 to 800 micrograms/m2 per day by intravenous infusion or 50 to 100 micrograms/m2 per day by subcutaneous injection and re-combinant human erythropoietin (rhEPO) in doses ranging from 2000 to 8000 IU/m2 per day by intravenous injection three times a week for at least 4 weeks. The goal was to evaluate whether therapy ameliorated pancytopenia in these patients as well as to determine its safety. All assessable patients showed a substantial increase in absolute neutrophil count, with a recovery of myeloid components (granulocyte series) in the bone marrow, after 2 to 10 weeks of treatment. An increase > 1.5 g/dL in hemoglobin (Hb) concentration was observed in 2 patients (10%). A decrease > 50% in red cell transfusion requirement was observed in 2 patients (10%). Seven patients showed recovery of neutropenia, anemia, and platelet count. In addition, there was no serious infection before or during therapy, and side effects were mild. Of the 20 patients, 3 showed a dramatic improvement in severe anemia after 10 weeks of treatment accompanying a recovery of erythroid components in the bone marrow. They no longer require red cell transfusions and have had normal Hb concentrations and normal ferrokinetics. These results indicate that long-term administration of rhG-CSF and rhEPO may benefit some patients with aplastic anemia. Further studies will be necessary to elucidate the mechanism by which rhGCSF and rhEPO stimulate hematopoiesis and improve hematologic abnormalities in these patients.", "gemini": "Hai mươi bệnh nhân bị thiếu máu bất sản đã được dùng thuốc kích thích tạo khuẩn lạc hạt bạch cầu của người tái tổ hợp (rhG-CSF) trong thời gian dài (10 tuần) với liều từ 50 đến 800 microgam/m2 mỗi ngày bằng cách truyền tĩnh mạch hoặc 50 đến 100 microgam/m2 mỗi ngày bằng cách tiêm dưới da và erythropoietin tái tổ hợp của người (rhEPO) với liều từ 2000 đến 8000 IU/m2 mỗi ngày bằng cách tiêm tĩnh mạch ba lần một tuần trong ít nhất 4 tuần. Mục tiêu là đánh giá xem liệu liệu pháp có cải thiện tình trạng giảm toàn thể các tế bào máu ở những bệnh nhân này cũng như xác định độ an toàn của liệu pháp. Tất cả các bệnh nhân có thể đánh giá đều cho thấy sự gia tăng đáng kể về số lượng bạch cầu trung tính tuyệt đối, với sự phục hồi của các thành phần tủy (dòng bạch cầu hạt) trong tủy xương, sau 2 đến 10 tuần điều trị. Sự gia tăng> 1,5 g/dL nồng độ hemoglobin (Hb) được quan sát thấy ở 2 bệnh nhân (10%). Sự giảm> 50% nhu cầu truyền hồng cầu được quan sát thấy ở 2 bệnh nhân (10%). Bảy bệnh nhân cho thấy sự phục hồi tình trạng giảm bạch cầu trung tính, thiếu máu và số lượng tiểu cầu. Ngoài ra, không có nhiễm trùng nghiêm trọng nào trước hoặc trong quá trình điều trị và các tác dụng phụ đều nhẹ. Trong số 20 bệnh nhân, 3 bệnh nhân cho thấy sự cải thiện đáng kể tình trạng thiếu máu nghiêm trọng sau 10 tuần điều trị đi kèm với sự phục hồi của các thành phần hồng cầu trong tủy xương. Họ không còn cần truyền hồng cầu và có nồng độ Hb bình thường và động học sắt bình thường. Những kết quả này chỉ ra rằng việc dùng rhG-CSF và rhEPO trong thời gian dài có thể có lợi cho một số bệnh nhân bị thiếu máu bất sản. Cần có thêm các nghiên cứu để làm sáng tỏ cơ chế mà rhGCSF và rhEPO kích thích quá trình tạo máu và cải thiện các bất thường về huyết học ở những bệnh nhân này."} +{"idx": 1950009, "en": "A common treatment regimen for patients with either insulin-dependent diabetes mellitus (IDDM) or non-insulin-dependent diabetes mellitus (NIDDM) is a combination of rapid-acting insulin and intermediate-acting insulin administered twice each day. It is usually recommended that regular human insulin be injected 30 to 45 minutes before a meal. In practice, patients often inject regular human insulin closer to mealtime, causing a higher post-prandial serum glucose level and an increased potential for hypoglycemia in the postabsorptive period. Insulin lispro, a rapid-acting insulin analogue, is best injected just before a meal because of its more rapid absorption and shorter duration of action. In 707 randomized patients, 379 with IDDM and 328 with NIDDM, we studied the effect of twice-daily insulin lispro or regular human insulin in combination with NPH human insulin (isophane insulin) on premeal, 2-hour postprandial, and bedtime glycemic control. Assessments were based on the results of a seven-point blood glucose profile, the insulin dose (by formulation and time of administration), the incidence and frequency of hypoglycemic episodes, and the glycated hemoglobin value. Treatment with insulin lispro resulted in lower postprandial glucose levels and smaller increases in glucose level after the morning and evening meals compared with treatment with regular human insulin. Overall glycemic control, frequency of hypoglycemic events, and total insulin dose were not different between the two groups. Insulin lispro in combination with NPH human insulin in a twice-per-day regimen allows injection closer to mealtime and improves post-prandial glycemic control without increasing the risk of hypoglycemia.", "gemini": "Một phác đồ điều trị thông thường cho bệnh nhân mắc bệnh đái tháo đường phụ thuộc insulin (IDDM) hoặc bệnh đái tháo đường không phụ thuộc insulin (NIDDM) là sự kết hợp giữa insulin tác dụng nhanh và insulin tác dụng trung gian được dùng hai lần mỗi ngày. Người ta thường khuyến cáo tiêm insulin người thường 30 đến 45 phút trước bữa ăn. Trong thực tế, bệnh nhân thường tiêm insulin người thường gần giờ ăn hơn, gây ra mức glucose huyết thanh sau ăn cao hơn và tăng khả năng hạ đường huyết trong giai đoạn sau hấp thu. Insulin lispro, một chất tương tự insulin tác dụng nhanh, được tiêm tốt nhất ngay trước bữa ăn vì hấp thu nhanh hơn và thời gian tác dụng ngắn hơn. Ở 707 bệnh nhân được phân ngẫu nhiên, 379 bệnh nhân mắc IDDM và 328 bệnh nhân mắc NIDDM, chúng tôi đã nghiên cứu tác dụng của insulin lispro hoặc insulin người thường dùng hai lần mỗi ngày kết hợp với insulin người NPH (insulin isophane) đối với kiểm soát đường huyết trước bữa ăn, sau ăn 2 giờ và trước khi đi ngủ. Đánh giá dựa trên kết quả của hồ sơ glucose máu bảy điểm, liều insulin (theo công thức và thời điểm dùng), tỷ lệ mắc và tần suất các cơn hạ đường huyết và giá trị hemoglobin glycosyl hóa. Điều trị bằng insulin lispro dẫn đến mức glucose sau ăn thấp hơn và mức tăng glucose nhỏ hơn sau bữa ăn sáng và tối so với điều trị bằng insulin người thường. Kiểm soát đường huyết toàn thể, tần suất các cơn hạ đường huyết và tổng liều insulin không khác nhau giữa hai nhóm. Insulin lispro kết hợp với insulin người NPH trong chế độ dùng hai lần mỗi ngày cho phép tiêm gần giờ ăn hơn và cải thiện kiểm soát đường huyết sau ăn mà không làm tăng nguy cơ hạ đường huyết."} +{"idx": 1950010, "en": "A total of 22 patients with acute ischemic stroke participated in two randomized, single-masked, placebo-controlled studies that evaluated the safety and pharmacokinetics of single escalating intravenous doses of lubeluzole. The first dose of study medication in all patients was given within 6 hours of the first sign of stroke onset. In the first study, 6 patients received a single 1-hour intravenous infusion of 5 mg of lubeluzole; 4 of these patients received an additional 10-mg dose 3 to 4 days later. Two additional patients received placebo. In the second study, 4 patients received a single 1-hour infusion of 10 mg of lubeluzole, and 2 patients received placebo. After a safety evaluation of the second study, 6 additional patients received 15 mg of lubeluzole, and 2 other patients received placebo. Lubeluzole had no clinically relevant effects on any cardiovascular variable compared with placebo. The majority of adverse experiences were mild to moderate and resolved during treatment. No unexpected electroencephalogram abnormalities were observed, and no evidence of epileptiform discharges was found in any of the patients. At the end of the infusion, plasma lubeluzole concentrations decayed biphasically, with mean distribution half-lives of 46.3 to 101.0 minutes and mean terminal half-lives of 20.8 to 27.7 hours. Comparisons of the dose-normalized value of the individual plasma concentrations at the end of the infusion and the total area under the curve from time 0 to infinity suggested that lubeluzole exhibited linear kinetics over the dose range evaluated in patients with ischemic stroke. In the small number of patients studied, lubeluzole's favorable safety profile was demonstrated by the lack of clinically relevant effects on cardiovascular variables and by neurologic examination and clinical laboratory findings.", "gemini": "Tổng cộng 22 bệnh nhân đột quỵ thiếu máu cục bộ cấp đã tham gia vào hai nghiên cứu ngẫu nhiên, mù đơn, có đối chứng giả dược để đánh giá tính an toàn và dược động học của các liều tiêm tĩnh mạch tăng dần của lubeluzole. Liều đầu tiên của thuốc nghiên cứu ở tất cả bệnh nhân được dùng trong vòng 6 giờ sau dấu hiệu đầu tiên của đột quỵ. Trong nghiên cứu đầu tiên, 6 bệnh nhân được truyền tĩnh mạch 1 giờ duy nhất 5 mg lubeluzole; 4 trong số những bệnh nhân này được dùng thêm liều 10 mg sau 3 đến 4 ngày. Hai bệnh nhân khác được dùng giả dược. Trong nghiên cứu thứ hai, 4 bệnh nhân được truyền tĩnh mạch 1 giờ duy nhất 10 mg lubeluzole và 2 bệnh nhân được dùng giả dược. Sau khi đánh giá độ an toàn của nghiên cứu thứ hai, 6 bệnh nhân khác được dùng 15 mg lubeluzole và 2 bệnh nhân khác được dùng giả dược. Lubeluzole không có tác dụng liên quan đến lâm sàng nào đối với bất kỳ biến số tim mạch nào so với giả dược. Phần lớn các tác dụng phụ đều nhẹ đến trung bình và hết trong quá trình điều trị. Không quan sát thấy bất kỳ bất thường nào về điện não đồ và không tìm thấy bằng chứng về các xung phóng điện dạng động kinh ở bất kỳ bệnh nhân nào. Vào cuối thời gian truyền, nồng độ lubeluzole trong huyết tương giảm theo hai pha, với thời gian bán hủy phân bố trung bình từ 46,3 đến 101,0 phút và thời gian bán hủy cuối trung bình từ 20,8 đến 27,7 giờ. So sánh giá trị chuẩn hóa liều của nồng độ huyết tương riêng lẻ vào cuối thời gian truyền và tổng diện tích dưới đường cong từ thời điểm 0 đến vô cùng cho thấy rằng lubeluzole biểu hiện động học tuyến tính trên phạm vi liều được đánh giá ở những bệnh nhân bị đột quỵ thiếu máu cục bộ. Ở số lượng nhỏ bệnh nhân được nghiên cứu, hồ sơ an toàn thuận lợi của lubeluzole đã được chứng minh bằng việc không có tác dụng liên quan đến lâm sàng đối với các biến số tim mạch và bằng các xét nghiệm lâm sàng và xét nghiệm cận lâm sàng về thần kinh."} +{"idx": 1950011, "en": "Health care decision-makers require more information on differences in compliance rates associated with alternative classes of antihypertensive drugs and the implications of these differences for health care utilization and costs. We examined medical claims data from the Pennsylvania Medicaid Management Information System to investigate compliance rates for four major antihypertensive drug classes (angiotensin-converting enzyme [ACE] inhibitors, beta-blockers, calcium antagonists, and diuretics) and the health care costs associated with noncompliance. Multivariate analysis was used to relate antihypertensive drug class with compliance and variations in compliance with health care costs. The highest estimated rates of compliance were associated with ACE inhibitors and calcium antagonists, and these rates were significantly greater than with beta-blockers and diuretics. Moreover, poor compliance was associated with higher health care costs. Efforts to increase compliance with antihypertensive drug therapy are needed to improve patient outcomes and reduce health care costs.", "gemini": "Những người ra quyết định chăm sóc sức khỏe cần có thêm thông tin về sự khác biệt về tỷ lệ tuân thủ liên quan đến các nhóm thuốc chống tăng huyết áp thay thế và ý nghĩa của những khác biệt này đối với việc sử dụng và chi phí chăm sóc sức khỏe. Chúng tôi đã kiểm tra dữ liệu yêu cầu bồi thường y tế từ Hệ thống thông tin quản lý Medicaid của Pennsylvania để điều tra tỷ lệ tuân thủ đối với bốn nhóm thuốc chống tăng huyết áp chính (thuốc ức chế men chuyển angiotensin [ACE], thuốc chẹn beta, thuốc đối kháng canxi và thuốc lợi tiểu) và chi phí chăm sóc sức khỏe liên quan đến việc không tuân thủ. Phân tích đa biến được sử dụng để liên hệ nhóm thuốc chống tăng huyết áp với sự tuân thủ và các biến thể về sự tuân thủ với chi phí chăm sóc sức khỏe. Tỷ lệ tuân thủ ước tính cao nhất có liên quan đến thuốc ức chế ACE và thuốc đối kháng canxi và tỷ lệ này cao hơn đáng kể so với thuốc chẹn beta và thuốc lợi tiểu. Hơn nữa, việc tuân thủ kém có liên quan đến chi phí chăm sóc sức khỏe cao hơn. Cần có những nỗ lực để tăng cường tuân thủ điều trị bằng thuốc chống tăng huyết áp nhằm cải thiện kết quả cho bệnh nhân và giảm chi phí chăm sóc sức khỏe."} +{"idx": 1950012, "en": "The purpose of this study was to assess medication compliance and to document side effects among a large number of Japanese patients receiving antihypertensive treatment. A total of 6289 patients being treated for hypertension returned completed questionnaires about their current blood pressure, therapy, side effects, and compliance with physicians' instructions. In addition, 4417 physicians returned completed questionnaires about their prescribing practices, side effects experienced by patients, and patient compliance. The antihypertensive agents they most often prescribed were calcium channel blockers, followed by angiotensin-converting enzyme inhibitors, beta-blockers, and diuretics. The proportion of patients who had well-controlled blood pressure, defined as systolic blood pressure < 160 mm Hg and diastolic blood pressure < 95 mm Hg, was similar regardless of the class of antihypertensive agent prescribed. Forty-nine percent of the patients with well-controlled blood pressure reported having at least one side effect while taking their current antihypertensive therapy, whereas a significantly greater percentage of patients (61%) with poorly controlled blood pressure reported side effects. Patients whose blood pressure was poorly controlled tended to have a higher incidence of most side effects than did those with well-controlled blood pressure. Also, the rate of intentional noncompliance was significantly higher in the group with poorly controlled blood pressure. In addition, the rate of noncompliance increased with the number of side effects reported. Although the causal relationship between side effects and non-compliance cannot be determined from this study, further investigation is warranted to better understand the impact these factors may have on overall cardiovascular morbidity and mortality.", "gemini": "Mục đích của nghiên cứu này là để đánh giá sự tuân thủ thuốc và ghi nhận các tác dụng phụ trong số lượng lớn bệnh nhân Nhật Bản đang điều trị tăng huyết áp. Tổng cộng 6289 bệnh nhân đang được điều trị tăng huyết áp đã trả lại các bảng câu hỏi đã hoàn thành về huyết áp hiện tại, liệu pháp, tác dụng phụ và sự tuân thủ hướng dẫn của bác sĩ. Ngoài ra, 4417 bác sĩ đã trả lại các bảng câu hỏi đã hoàn thành về các hoạt động kê đơn của họ, các tác dụng phụ mà bệnh nhân gặp phải và sự tuân thủ của bệnh nhân. Các thuốc chống tăng huyết áp mà họ thường kê đơn nhất là thuốc chẹn kênh canxi, tiếp theo là thuốc ức chế men chuyển angiotensin, thuốc chẹn beta và thuốc lợi tiểu. Tỷ lệ bệnh nhân có huyết áp được kiểm soát tốt, được định nghĩa là huyết áp tâm thu <160 mm Hg và huyết áp tâm trương <95 mm Hg, là tương tự nhau bất kể loại thuốc chống tăng huyết áp nào được kê đơn. Bốn mươi chín phần trăm bệnh nhân có huyết áp được kiểm soát tốt báo cáo có ít nhất một tác dụng phụ khi dùng liệu pháp chống tăng huyết áp hiện tại, trong khi tỷ lệ phần trăm lớn hơn đáng kể bệnh nhân (61%) có huyết áp kiểm soát kém báo cáo tác dụng phụ. Những bệnh nhân có huyết áp kiểm soát kém có xu hướng có tỷ lệ mắc hầu hết các tác dụng phụ cao hơn so với những bệnh nhân có huyết áp được kiểm soát tốt. Ngoài ra, tỷ lệ không tuân thủ cố ý cao hơn đáng kể ở nhóm có huyết áp kiểm soát kém. Ngoài ra, tỷ lệ không tuân thủ tăng theo số lượng tác dụng phụ được báo cáo. Mặc dù mối quan hệ nhân quả giữa tác dụng phụ và không tuân thủ không thể xác định được từ nghiên cứu này, nhưng cần phải điều tra thêm để hiểu rõ hơn về tác động của các yếu tố này đối với tỷ lệ mắc bệnh tim mạch và tử vong nói chung."} +{"idx": 1950013, "en": "Schizophrenia is arguably one of the most costly mental illnesses in terms of its impact on the economy, on the health system, and on patients and their families. This paper provides a framework for analyzing schizophrenia; this framework, in a limited data environment, generates estimates of the costs and outcomes of this disease based on location of treatment. This model differs from previous cost-of-illness studies in that it uses a Markov framework to estimate the incidence costs associated with schizophrenia based on a projected amount of a patient's lifetime spent in these treatment locations. The author believes this is the first time such a model has been developed and applied to estimating not only the community costs of schizophrenia but also the cost implications of treatment options and policy choices in Australia. Using a fundamental matrix solution, lifetime costs of illness are calculated and the principal cost drivers in schizophrenia treatment are identified.", "gemini": "Có thể lập luận rằng tâm thần phân liệt là một trong những bệnh tâm thần tốn kém nhất xét về tác động của nó đối với nền kinh tế, hệ thống y tế, bệnh nhân và gia đình của họ. Bài báo này cung cấp một khuôn khổ để phân tích bệnh tâm thần phân liệt; khuôn khổ này, trong một môi trường dữ liệu hạn chế, tạo ra các ước tính về chi phí và kết quả của căn bệnh này dựa trên địa điểm điều trị. Mô hình này khác với các nghiên cứu về chi phí bệnh tật trước đây ở chỗ nó sử dụng khuôn khổ Markov để ước tính chi phí mắc phải liên quan đến bệnh tâm thần phân liệt dựa trên số tiền dự kiến trong suốt cuộc đời của bệnh nhân tại các địa điểm điều trị này. Tác giả tin rằng đây là lần đầu tiên một mô hình như vậy được phát triển và áp dụng để ước tính không chỉ chi phí cộng đồng của bệnh tâm thần phân liệt mà còn cả những tác động về chi phí của các lựa chọn điều trị và chính sách tại Úc. Sử dụng một giải pháp ma trận cơ bản, chi phí suốt đời của bệnh tật được tính toán và các yếu tố thúc đẩy chi phí chính trong điều trị bệnh tâm thần phân liệt được xác định."} +{"idx": 1950014, "en": "The use of nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with a 2% to 4% annual incidence of serious gastrointestinal complications. These adverse clinical outcomes, and the strategies used to prevent their occurrence, translate into a significant economic burden. A decision-analysis model was constructed to contrast the 6-month costs associated with various approaches to preventing and managing NSAID-induced gastropathy and to evaluate the economic impact of two treatment regimens using fixed-dose formulations of diclofenac/misoprostol. After incorporating expected medical out-comes and predicted practice patterns, 6-month per-patient costs were derived from the model for each of five treatment regimens: (1) NSAID alone; (2) NSAID with a histamine2-receptor antagonist; (3) NSAID with coprescribed misoprostol; (4) diclofenac/misoprostol 50 mg/200 micrograms TID/BID; and (5) diclofenac/misoprostol 75 mg/200 micrograms BID. The combined diclofenac/misoprostol regimens demonstrated an 18.6% per-patient cost advantage compared with the combined NSAID regimens. Based on a 6-month period, this cost savings translated into a $214.00 per-patient overall cost savings ($1153.00 per patient for NSAID regimens versus $939.00 for diclofenac/misoprostol regimens). The magnitude of this difference was verified by Monte Carlo simulation. Despite the considerable cost difference, sensitivity analyses revealed that our model was robust and that no single variation substantially influenced the results. Given the lack of long-term prospective, comparative clinical-outcomes studies in this area, this decision analysis provides guidance to clinicians in developing a rational and cost-effective approach to the treatment of patients requiring chronic NSAID therapy.", "gemini": "Việc sử dụng các thuốc chống viêm không steroid (NSAID) có liên quan đến tỷ lệ mắc các biến chứng nghiêm trọng ở đường tiêu hóa từ 2% đến 4% hàng năm. Những kết quả lâm sàng bất lợi này và các chiến lược được sử dụng để ngăn ngừa chúng xảy ra sẽ tạo thành một gánh nặng kinh tế đáng kể. Một mô hình phân tích quyết định đã được xây dựng để đối chiếu các chi phí trong 6 tháng liên quan đến các phương pháp khác nhau để ngăn ngừa và kiểm soát bệnh dạ dày do NSAID gây ra và để đánh giá tác động kinh tế của hai chế độ điều trị sử dụng các chế phẩm liều cố định diclofenac/misoprostol. Sau khi kết hợp các kết quả y tế dự kiến và các mô hình thực hành được dự đoán, chi phí cho mỗi bệnh nhân trong 6 tháng được lấy từ mô hình cho mỗi chế độ điều trị trong số năm chế độ điều trị: (1) Chỉ NSAID; (2) NSAID với thuốc đối kháng thụ thể histamine2; (3) NSAID với misoprostol được kê kèm; (4) diclofenac/misoprostol 50 mg/200 microgam TID/BID; và (5) diclofenac/misoprostol 75 mg/200 microgam BID. Các chế độ diclofenac/misoprostol kết hợp cho thấy lợi thế về chi phí cho mỗi bệnh nhân là 18,6% so với các chế độ NSAID kết hợp. Dựa trên giai đoạn 6 tháng, khoản tiết kiệm chi phí này tương đương với khoản tiết kiệm chi phí chung cho mỗi bệnh nhân là 214,00 đô la (1153,00 đô la cho mỗi bệnh nhân đối với các chế độ NSAID so với 939,00 đô la cho các chế độ diclofenac/misoprostol). Quy mô của sự khác biệt này đã được xác minh bằng mô phỏng Monte Carlo. Bất chấp sự khác biệt đáng kể về chi phí, các phân tích độ nhạy cho thấy mô hình của chúng tôi rất mạnh mẽ và không có biến thể nào ảnh hưởng đáng kể đến kết quả. Do thiếu các nghiên cứu so sánh kết quả lâm sàng có triển vọng dài hạn trong lĩnh vực này, nên phân tích quyết định này cung cấp hướng dẫn cho các bác sĩ lâm sàng trong việc phát triển một phương pháp hợp lý và hiệu quả về chi phí để điều trị bệnh nhân cần dùng NSAID mạn tính."} +{"idx": 1950015, "en": "As policy makers explore ways to reform the Medicare system, the inevitable question of prescription drug coverage for the elderly will surface. This is an opportunity to revisit and balance the attractiveness of including a prescription benefit against the fear of increases in expenditures. Prudent examination of the costs and benefits of prescription drug coverage requires looking beyond pharmaceutical product costs.", "gemini": "Khi các nhà hoạch định chính sách khám phá các cách cải cách hệ thống Medicare, câu hỏi không thể tránh khỏi về phạm vi bảo hiểm thuốc theo toa cho người cao tuổi sẽ nổi lên. Đây là cơ hội để xem xét lại và cân bằng sức hấp dẫn của việc đưa vào một lợi ích theo toa so với nỗi sợ gia tăng chi phí. Việc xem xét thận trọng các chi phí và lợi ích của phạm vi bảo hiểm thuốc theo toa đòi hỏi phải xem xét vượt ra ngoài chi phí sản phẩm dược phẩm."} +{"idx": 1950016, "en": "The need to evaluate the effects of health technologies in clinical practice is increasingly important. In this article, we review the advantages and limitations of naturalistic randomized clinical trials (RCTs) and database analyses, the two primary methods for evaluating treatment effectiveness. Also, we comment on a newer research strategy, cross-design synthesis, which proposes the complementary use of both experimental RCTs and observational database methodologies to avoid the main weaknesses of each: respectively, the lack of external and internal validity. Finally, we propose a new strategy--randomized database studies--capable of generating results with an acceptable balance between internal and external validity. This strategy consists of the simultaneous use of both experimental and observational tools in the assessment of drugs' effectiveness. Randomization is essential to minimize comparison bias, and one possibility for such studies is that randomization modules could be included in computer-based patient records. Although we identify some of the difficulties in implementing the process, the progressive standardization of clinical practice and the development and widespread adoption of improved computer-based patient records could facilitate the use of randomized database studies as a new method of research.", "gemini": "Nhu cầu đánh giá tác dụng của các công nghệ y tế trong thực hành lâm sàng ngày càng trở nên quan trọng. Trong bài viết này, chúng tôi xem xét những ưu điểm và hạn chế của các thử nghiệm lâm sàng ngẫu nhiên theo tự nhiên (RCT) và phân tích cơ sở dữ liệu, hai phương pháp chính để đánh giá hiệu quả điều trị. Ngoài ra, chúng tôi nhận xét về một chiến lược nghiên cứu mới hơn, tổng hợp thiết kế chéo, đề xuất sử dụng bổ sung cả RCT thực nghiệm và phương pháp cơ sở dữ liệu quan sát để tránh các điểm yếu chính của từng phương pháp: tương ứng là thiếu giá trị bên ngoài và bên trong. Cuối cùng, chúng tôi đề xuất một chiến lược mới - các nghiên cứu cơ sở dữ liệu ngẫu nhiên - có khả năng tạo ra kết quả với sự cân bằng chấp nhận được giữa giá trị bên trong và bên ngoài. Chiến lược này bao gồm việc sử dụng đồng thời cả công cụ thực nghiệm và quan sát trong việc đánh giá hiệu quả của thuốc. Ngẫu nhiên là điều cần thiết để giảm thiểu sai lệch so sánh và một khả năng cho các nghiên cứu như vậy là các mô-đun ngẫu nhiên có thể được đưa vào hồ sơ bệnh nhân trên máy tính. Mặc dù chúng tôi xác định một số khó khăn trong việc triển khai quy trình, nhưng việc chuẩn hóa dần dần thực hành lâm sàng và sự phát triển và áp dụng rộng rãi các hồ sơ bệnh nhân trên máy tính được cải tiến có thể tạo điều kiện thuận lợi cho việc sử dụng các nghiên cứu cơ sở dữ liệu ngẫu nhiên như một phương pháp nghiên cứu mới."} +{"idx": 1950017, "en": "A patient suffering from semantic dementia is described who consistently demonstrated the preserved ability to support specific types of semantic judgements from visual, but not from verbal, input. In addition the representations accessed from visual input were found to trigger complex behavioural schemata, while with verbal materials the patients performed almost invariably at chance level. A preliminary description is given of the nature of visual semantic representations, and the privileged relationship between this modality of input and some aspects of semantic knowledge is also explored. The richness of the semantic representations accessed from visual input can be accommodated within the \"Multimodal Semantics\" framework; alternative views, derived from the Identification Semantics and the Organized Unitary Content Hypothesis, are also discussed.", "gemini": "Mô tả một bệnh nhân mắc chứng mất trí nhớ ngữ nghĩa, người luôn thể hiện khả năng được bảo tồn để hỗ trợ các loại phán đoán ngữ nghĩa cụ thể từ đầu vào trực quan, nhưng không phải từ đầu vào bằng lời nói. Ngoài ra, các biểu diễn truy cập từ đầu vào trực quan được phát hiện là kích hoạt các lược đồ hành vi phức tạp, trong khi với các tài liệu bằng lời nói, bệnh nhân gần như luôn thực hiện ở mức ngẫu nhiên. Một mô tả sơ bộ được đưa ra về bản chất của các biểu diễn ngữ nghĩa trực quan và mối quan hệ đặc quyền giữa phương thức đầu vào này và một số khía cạnh của kiến thức ngữ nghĩa cũng được khám phá. Sự phong phú của các biểu diễn ngữ nghĩa được truy cập từ đầu vào trực quan có thể được điều chỉnh trong khuôn khổ \"Ngữ nghĩa đa phương thức\"; các quan điểm thay thế, bắt nguồn từ Ngữ nghĩa nhận dạng và Giả thuyết nội dung thống nhất có tổ chức, cũng được thảo luận."} +{"idx": 1950018, "en": "We report a patient who showed a dissociation between impaired semantic memory and preserved autobiographic memory. M.N., a 56-year-old right-handed woman, developed a supra sellar meningioma and underwent multiple operations. Following Lineac radiation, necrosis occurred in the left temporal lobe. Magnetic Resonance Imaging revealed bilateral lesions in the temporal lobes and in the right basal frontal lobe. The patient's intellectual deterioration and anterograde amnesia were mild. Language was preserved, but for a subtle anomia. Autobiographic memory remained intact, while semantic memory for public events, historical figures, cultural items, knowledge of low frequency words and technical terms related to her profession was severely impaired. We hypothesize that bilateral lesions of the anterior half of the middle region of the temporal lobe plays a crucial role in causing deficits in semantic memory.", "gemini": "Chúng tôi báo cáo về một bệnh nhân cho thấy sự phân ly giữa trí nhớ ngữ nghĩa bị suy yếu và trí nhớ tự truyện được bảo tồn. M.N., một phụ nữ thuận tay phải 56 tuổi, đã phát triển một khối u màng não trên yên và đã trải qua nhiều ca phẫu thuật. Sau khi xạ trị bằng Lineac, hoại tử xảy ra ở thùy thái dương trái. Chụp cộng hưởng từ cho thấy tổn thương ở cả hai bên thùy thái dương và ở thùy trán nền bên phải. Bệnh nhân bị suy giảm trí tuệ và mất trí nhớ tiến triển nhẹ. Ngôn ngữ được bảo tồn, nhưng có một chứng mất tên nhẹ. Trí nhớ tự truyện vẫn còn nguyên vẹn, trong khi trí nhớ ngữ nghĩa về các sự kiện công cộng, nhân vật lịch sử, các vật phẩm văn hóa, kiến thức về các từ tần suất thấp và các thuật ngữ kỹ thuật liên quan đến nghề nghiệp của cô bị suy giảm nghiêm trọng. Chúng tôi đưa ra giả thuyết rằng các tổn thương ở cả hai bên của nửa trước vùng giữa của thùy thái dương đóng vai trò rất quan trọng trong việc gây ra tình trạng thiếu hụt trí nhớ ngữ nghĩa."} +{"idx": 1950019, "en": "This paper explores how one health care purchasing coalition in Minnesota, the Buyers Health Care Action Group (BHCAG), has taken an active role in restructuring its local health care market. BHCAG started with the belief that the consumer should be the motivating force in health care delivery. Unfortunately, providing consumers with the information and incentives they need to make informed, effective health care decisions has triggered numerous problems. This paper examines groups of providers who network to form care systems, and explores the roles of consumers, employers, and health plans in the current market. It identifies specific methods for gathering data and distributing information to the consumer, and discusses the problems associated with attempting to implement quality improvement, as well as the questions that arise when the market does not support those improvements.", "gemini": "Bài báo này khám phá cách một liên minh mua sắm chăm sóc sức khỏe ở Minnesota, Buyers Health Care Action Group (BHCAG), đã đóng vai trò tích cực trong việc tái cơ cấu thị trường chăm sóc sức khỏe địa phương của mình. BHCAG bắt đầu với niềm tin rằng người tiêu dùng nên là động lực thúc đẩy việc cung cấp dịch vụ chăm sóc sức khỏe. Thật không may, việc cung cấp cho người tiêu dùng thông tin và các ưu đãi mà họ cần để đưa ra các quyết định chăm sóc sức khỏe hiệu quả, có hiểu biết đã gây ra nhiều vấn đề. Bài báo này xem xét các nhóm nhà cung cấp kết nối mạng để hình thành hệ thống chăm sóc và khám phá vai trò của người tiêu dùng, người sử dụng lao động và các kế hoạch sức khỏe trên thị trường hiện tại. Nó xác định các phương pháp cụ thể để thu thập dữ liệu và phân phối thông tin đến người tiêu dùng, đồng thời thảo luận về các vấn đề liên quan đến việc cố gắng thực hiện cải tiến chất lượng, cũng như các câu hỏi nảy sinh khi thị trường không hỗ trợ những cải tiến đó."} +{"idx": 1950020, "en": "Using the California Public Employees' Retirement System (CalPERS) as an example, this paper illustrates how an employer can abandon the more traditional role of passive purchaser of health care benefits and become an active participant in cost containment and quality improvement in the health care industry. Through the use of the Health Plan Employer Data Information Set (HEDIS) 3.0 and other data collection and reporting methods, the communication gap between consumers, purchasers, health plans, and providers is narrowing. Increasingly, purchasers like CalPERS are becoming involved in quality improvement; they are recognizing the need to serve as intermediaries between consumers and health plans, to understand available data, and to disseminate that information to the consumer. The purchaser's impact on the health care community's business practices is growing. As a result, the collection and reporting of HEDIS data are now emerging as substantial fiduciary reporting responsibilities for health plans. This paper outlines a plan implemented in California for improving HEDIS data gathering and examines surveys, results, and problems encountered in improving and integrating health care information.", "gemini": "Sử dụng Hệ thống hưu trí của công nhân viên chức California (CalPERS) làm ví dụ, bài báo này minh họa cách một người sử dụng lao động có thể từ bỏ vai trò truyền thống hơn là người mua thụ động các quyền lợi chăm sóc sức khỏe và trở thành người tham gia tích cực vào việc kiểm soát chi phí và cải tiến chất lượng trong ngành chăm sóc sức khỏe. Thông qua việc sử dụng Bộ dữ liệu thông tin về người sử dụng lao động của Kế hoạch sức khỏe (HEDIS) 3.0 và các phương pháp thu thập và báo cáo dữ liệu khác, khoảng cách giao tiếp giữa người tiêu dùng, người mua, các kế hoạch sức khỏe và nhà cung cấp đang thu hẹp lại. Ngày càng có nhiều người mua như CalPERS tham gia vào việc cải tiến chất lượng; họ nhận ra nhu cầu đóng vai trò trung gian giữa người tiêu dùng và các kế hoạch sức khỏe, để hiểu dữ liệu có sẵn và truyền bá thông tin đó đến người tiêu dùng. Tác động của người mua đối với các hoạt động kinh doanh của cộng đồng chăm sóc sức khỏe đang ngày càng lớn. Do đó, việc thu thập và báo cáo dữ liệu HEDIS hiện đang nổi lên như một trách nhiệm báo cáo ủy thác đáng kể đối với các kế hoạch sức khỏe. Bài báo này phác thảo một kế hoạch được triển khai tại California để cải thiện việc thu thập dữ liệu HEDIS và xem xét các cuộc khảo sát, kết quả và các vấn đề gặp phải trong việc cải thiện và tích hợp thông tin chăm sóc sức khỏe."} +{"idx": 1950021, "en": "Ms D., a 76 year-old woman with a slowly progressive bilateral parietal lobe degeneration, showed an unusual variant of misreaching as yet unreported. When required to reach to a target in extrafoveal vision, she slavishly reached straight to the foveal fixation point instead (\"magnetic misreaching\"). Three dimensional recordings of limb movements to foveal and extrafoveal targets revealed that her reach endpoints were determined by the place she was looking, independent of the distance between target and fixation point. The sign was present in both hands. Magnetic misreaching differs from motor misbehaviours which follow frontal lobe damage. The neuropsychological and behavioural profile of Ms D., coupled with neuroimaging evidence suggest that magnetic misreaching is a manifestation of parietal lobe dysfunction.", "gemini": "Bà D., một phụ nữ 76 tuổi mắc chứng thoái hóa thùy đỉnh hai bên tiến triển chậm, đã biểu hiện một biến thể bất thường của chứng với sai sót khi với tới mà chưa được báo cáo. Khi được yêu cầu với tới một mục tiêu trong tầm nhìn ngoài hoàng điểm, bà đã ngoan cố với thẳng đến điểm cố định hoàng điểm thay vì (\"sai sót khi với tới do từ tính\"). Các bản ghi ba chiều về chuyển động của chi đến các mục tiêu hoàng điểm và ngoài hoàng điểm cho thấy điểm cuối với tới của bà được xác định bởi nơi bà nhìn, không phụ thuộc vào khoảng cách giữa mục tiêu và điểm cố định. Dấu hiệu này xuất hiện ở cả hai tay. Sai sót khi với tới do từ tính khác với các hành vi sai sót về vận động do tổn thương thùy trán. Hồ sơ hành vi và tâm lý thần kinh của bà D., cùng với bằng chứng chụp ảnh thần kinh cho thấy rằng sai sót khi với tới do từ tính là biểu hiện của rối loạn chức năng thùy đỉnh."} +{"idx": 1950022, "en": "In recent years, the Joint Commission on Accreditation of Healthcare Organizations (JCAHO) has expanded its traditional role as a leading evaluator of health care organizations to incorporate new attitudes and strategies in the areas of quality and performance improvement. This paper outlines plans for modifying the current accreditation process, and includes a proposition that accredited organizations actively participate in a quality measurement system and become involved in the process of selecting appropriate measures. The paper presents current and proposed methods for measuring performance standards, compares major accreditation systems, and explores the public's expectations for health care quality and the JCAHO's role in quality improvement and cost containment.", "gemini": "Trong những năm gần đây, Ủy ban chung về công nhận các tổ chức chăm sóc sức khỏe (JCAHO) đã mở rộng vai trò truyền thống của mình là tổ chức đánh giá hàng đầu các tổ chức chăm sóc sức khỏe để kết hợp các thái độ và chiến lược mới trong các lĩnh vực cải tiến chất lượng và hiệu suất. Bài báo này phác thảo các kế hoạch sửa đổi quy trình công nhận hiện tại và bao gồm đề xuất rằng các tổ chức được công nhận tích cực tham gia vào hệ thống đo lường chất lượng và tham gia vào quá trình lựa chọn các biện pháp phù hợp. Bài báo trình bày các phương pháp hiện tại và được đề xuất để đo lường các tiêu chuẩn hiệu suất, so sánh các hệ thống công nhận chính và khám phá kỳ vọng của công chúng đối với chất lượng chăm sóc sức khỏe và vai trò của JCAHO trong việc cải thiện chất lượng và kiềm chế chi phí."} +{"idx": 1950023, "en": "This paper explores the idea of accountability in the managed care arena and the need for employers to demand accountability if they are to establish themselves as customers. Four key principles for establishing greater accountability are outlined: addressing health care issues at the local level; focusing on quality of health care as well as cost; collecting data on quality; and creating an accountable health care system through value-based purchasing. A review of methods for establishing accountability when negotiating contracts with managed care organizations (MCOs) focuses on the significance of collecting and understanding claims data, defining performance standards, and establishing and using MCO incentives.", "gemini": "Bài báo này khám phá ý tưởng về trách nhiệm giải trình trong lĩnh vực chăm sóc được quản lý và nhu cầu các nhà tuyển dụng yêu cầu trách nhiệm giải trình nếu họ muốn khẳng định mình là khách hàng. Bốn nguyên tắc chính để thiết lập trách nhiệm giải trình lớn hơn được nêu ra: giải quyết các vấn đề chăm sóc sức khỏe ở cấp địa phương; tập trung vào chất lượng chăm sóc sức khỏe cũng như chi phí; thu thập dữ liệu về chất lượng; và tạo ra một hệ thống chăm sóc sức khỏe có trách nhiệm giải trình thông qua việc mua hàng dựa trên giá trị. Việc xem xét các phương pháp thiết lập trách nhiệm giải trình khi đàm phán hợp đồng với các tổ chức chăm sóc được quản lý (MCO) tập trung vào tầm quan trọng của việc thu thập và hiểu dữ liệu yêu cầu bồi thường, xác định các tiêu chuẩn hiệu suất và thiết lập và sử dụng các ưu đãi của MCO."} +{"idx": 1950024, "en": "On the surface, quality assurance and quality improvement appear to have similar objectives; however, the purpose of each is fundamentally different. This paper discusses these two frequently confused approaches to quality in health care, identifying the primary objective of each and clarifying which approach should be used for various situations. Past and present issues, including questions such as, \"What should a health plan's role be?\" and \"What does the public want?\" are explored using examples and study results. A California quality scoreboard project is also discussed, including the initial process of ranking plans and the difficulties encountered in attempting to publicize the data. Finally, areas of consensus and debate are addressed.", "gemini": "Trên bề mặt, đảm bảo chất lượng và cải tiến chất lượng có vẻ có các mục tiêu tương tự; tuy nhiên, mục đích của mỗi mục tiêu về cơ bản là khác nhau. Bài báo này thảo luận về hai cách tiếp cận thường bị nhầm lẫn này đối với chất lượng trong chăm sóc sức khỏe, xác định mục tiêu chính của mỗi cách tiếp cận và làm rõ cách tiếp cận nào nên được sử dụng cho các tình huống khác nhau. Các vấn đề trong quá khứ và hiện tại, bao gồm các câu hỏi như \"Vai trò của một kế hoạch chăm sóc sức khỏe là gì?\" và \"Công chúng muốn gì?\" được khám phá bằng cách sử dụng các ví dụ và kết quả nghiên cứu. Một dự án bảng điểm chất lượng của California cũng được thảo luận, bao gồm quá trình xếp hạng ban đầu và những khó khăn gặp phải khi cố gắng công khai dữ liệu. Cuối cùng, các lĩnh vực đồng thuận và tranh luận được giải quyết."} +{"idx": 1950025, "en": "Repetition of single words and pronounceable nonwords (pseudowords) was assessed in Alzheimer's Disease (AD) patients to evaluate how lexical phonological processing might be accomplished when semantic and conceptual knowledge is impaired. AD patients performed significantly worse than healthy elderly controls on all repetition tasks. However, repetition abilities and dementia severity were not correlated, and AD patients produced the same distribution of error types as controls. Furthermore, despite their semantic problems, AD patients, like controls, showed a significant advantage for repeating real words compared to pseudowords, even when repeating low frequency phonologically complex words whose meaning is not likely to have been retained. The results support the postulated existence of a lexical phonological system that is used to repeat both known and novel words and that processes linguistic information independent of its meaning.", "gemini": "Sự lặp lại của các từ đơn lẻ và các từ không thể phát âm được (từ giả) đã được đánh giá ở những bệnh nhân mắc bệnh Alzheimer (AD) để đánh giá cách xử lý âm vị từ vựng có thể được thực hiện như thế nào khi kiến thức ngữ nghĩa và khái niệm bị suy yếu. Những bệnh nhân mắc bệnh AD thực hiện kém hơn đáng kể so với những người cao tuổi khỏe mạnh trong tất cả các nhiệm vụ lặp lại. Tuy nhiên, khả năng lặp lại và mức độ nghiêm trọng của chứng mất trí nhớ không tương quan, và những bệnh nhân mắc bệnh AD tạo ra cùng một sự phân bố các loại lỗi như những người đối chứng. Hơn nữa, mặc dù có vấn đề về ngữ nghĩa, những bệnh nhân mắc bệnh AD, giống như những người đối chứng, đã cho thấy một lợi thế đáng kể khi lặp lại các từ thực so với các từ giả, ngay cả khi lặp lại các từ phức tạp về mặt âm vị có tần suất thấp mà ý nghĩa của chúng không có khả năng được giữ lại. Kết quả hỗ trợ cho sự tồn tại được giả định của một hệ thống âm vị từ vựng được sử dụng để lặp lại cả các từ đã biết và các từ mới và xử lý thông tin ngôn ngữ độc lập với ý nghĩa của nó."} +{"idx": 1950026, "en": "This paper discusses the role of the Agency for Health Care Policy and Research (AHCPR) in identifying and solving problems within the health care industry. It reviews current AHCPR activities and details the results of the Consumer Assessment of Health Plans Survey, the recent poll of how people in the United States make choices about health care. A discussion of present quality measurement tools, such as CONQUEST, a database of existing performance measures, includes an outline of plans for developing new measures of quality and outcomes. Also explored are ideas for improving quality measurements, potential problems and risks, and the public role in health services research.", "gemini": "Bài báo này thảo luận về vai trò của Cơ quan Chính sách và Nghiên cứu Chăm sóc Sức khỏe (AHCPR) trong việc xác định và giải quyết các vấn đề trong ngành chăm sóc sức khỏe. Nó xem xét các hoạt động hiện tại của AHCPR và nêu chi tiết kết quả của Khảo sát đánh giá của người tiêu dùng về các kế hoạch chăm sóc sức khỏe, cuộc thăm dò gần đây về cách mọi người ở Hoa Kỳ đưa ra lựa chọn về chăm sóc sức khỏe. Một cuộc thảo luận về các công cụ đo lường chất lượng hiện tại, chẳng hạn như CONQUEST, cơ sở dữ liệu về các biện pháp hiệu suất hiện có, bao gồm phác thảo các kế hoạch phát triển các biện pháp mới về chất lượng và kết quả. Ngoài ra còn khám phá các ý tưởng để cải thiện các phép đo chất lượng, các vấn đề và rủi ro tiềm ẩn và vai trò của công chúng trong nghiên cứu dịch vụ y tế."} +{"idx": 1950027, "en": "Patients with left temporal lobe epilepsy (LTLE) have been shown to demonstrate impaired categorization in semantic memory tasks leaving open the question whether this is due to problems with active categorization or to problems with consolidation/retrieval of relations. 35 patients with left or right temporal lobe epilepsy (LTLE, RTLE) and 20 healthy controls performed three verbal learning and memory tests differing in the relational distance between the items to be learned (unrelated, low associative, high associative). In order to separate the memory component in relational memory processing, we avoided problems in active categorization and perception of semantic relations by presenting category members already clustered in the order of category membership, and by checking whether the patients had actually noticed the categories. In controls and in patients with RTLE, both semantic conditions significantly improved the test performance. LTLE-patients showed the worst test performance independent of the test condition. Their performance improved under the high associative condition, but they did not at all profit from the low associative condition. Although the semantic tasks had no demand on active clustering and although all LTLE-patients noticed the relational properties very well, they could not make use of the offered clusters. They demonstrated significantly more \"item specific\" than \"relational\" encoding than the other groups. This was pronounced in the low associative condition but became evident also in the high associative condition. In contrast to the other groups, the order in recall (clustering) deteriorated with passing time in LTLE-patients. In conclusion the data provide evidence of impaired semantic relational processing in LTLE. The impairment depends on the relational properties of the material to be learned, in that it increases with the relational distance between the memory contents. Because the tasks did not demand active clustering, we can attribute this impairment to deficits in relational encoding/retrieval which would be consistent with current opinions of the role of temporolimbic structures in relational memory processing.", "gemini": "Những bệnh nhân bị động kinh thùy thái dương trái (LTLE) đã được chứng minh là biểu hiện sự phân loại kém trong các nhiệm vụ trí nhớ ngữ nghĩa khiến câu hỏi liệu điều này là do vấn đề với việc phân loại chủ động hay vấn đề với việc củng cố/truy xuất các mối quan hệ vẫn còn bỏ ngỏ. 35 bệnh nhân bị động kinh thùy thái dương trái hoặc phải (LTLE, RTLE) và 20 người đối chứng khỏe mạnh đã thực hiện ba bài kiểm tra học tập và ghi nhớ bằng lời khác nhau về khoảng cách quan hệ giữa các mục cần học (không liên quan, liên kết thấp, liên kết cao). Để tách thành phần trí nhớ trong quá trình xử lý trí nhớ quan hệ, chúng tôi đã tránh các vấn đề trong việc phân loại chủ động và nhận thức các mối quan hệ ngữ nghĩa bằng cách trình bày các thành viên của thể loại đã được nhóm theo thứ tự thành viên thể loại và bằng cách kiểm tra xem bệnh nhân có thực sự nhận ra các thể loại hay không. Ở nhóm đối chứng và ở những bệnh nhân bị RTLE, cả hai điều kiện ngữ nghĩa đều cải thiện đáng kể hiệu suất kiểm tra. Bệnh nhân LTLE cho thấy hiệu suất kiểm tra kém nhất không phụ thuộc vào điều kiện kiểm tra. Hiệu suất của họ được cải thiện trong điều kiện liên kết cao, nhưng họ hoàn toàn không hưởng lợi từ điều kiện liên kết thấp. Mặc dù các nhiệm vụ ngữ nghĩa không có nhu cầu về nhóm cụm chủ động và mặc dù tất cả bệnh nhân LTLE đều nhận thấy các đặc tính quan hệ rất tốt, nhưng họ không thể sử dụng các cụm được cung cấp. Họ chứng minh được nhiều mã hóa \"cụ thể theo mục\" hơn là mã hóa \"quan hệ\" hơn các nhóm khác. Điều này được thể hiện rõ trong điều kiện liên kết thấp nhưng cũng trở nên rõ ràng trong điều kiện liên kết cao. Trái ngược với các nhóm khác, thứ tự nhớ lại (nhóm cụm) xấu đi theo thời gian ở những bệnh nhân LTLE. Tóm lại, dữ liệu cung cấp bằng chứng về quá trình xử lý quan hệ ngữ nghĩa bị suy yếu ở LTLE. Sự suy giảm phụ thuộc vào các đặc tính quan hệ của tài liệu cần học, ở chỗ nó tăng lên theo khoảng cách quan hệ giữa các nội dung trí nhớ. Vì các nhiệm vụ không yêu cầu nhóm cụm chủ động, nên chúng tôi có thể quy sự suy giảm này cho những thiếu hụt trong việc mã hóa/truy xuất quan hệ, điều này sẽ phù hợp với quan điểm hiện tại về vai trò của các cấu trúc thái dương trong quá trình xử lý trí nhớ quan hệ."} +{"idx": 1950028, "en": "The Chronic Illness Management Research and Development Project (CIMRDP) was created as part of an effort to implement clinical process improvements in HealthSystems Minnesota, a vertically integrated care system in Minneapolis. CIMRDP's objective is to introduce sustainable improvements in the management of chronic illness. These types of quality improvement efforts require fundamental change at the organizational level, which often does not occur, and the programs do not continue beyond the initial stage. Using CIMRDP as a model for a sustainable chronic illness management program, this paper explores the process of implementing clinical guidelines for the management of chronic illness and contending with the complex problem of initiating change at an organizational level. It reviews factors critical to the success of such a program, including the use of focus groups to ensure a patient-centered approach, the advantage of hiring an outside consultant, the question of whether to accept external funding, and an evaluation outline of the six main tasks the program requires. Methods for marketing and integration are also presented.", "gemini": "Dự án Nghiên cứu và Phát triển Quản lý Bệnh mãn tính (CIMRDP) được tạo ra như một phần của nỗ lực thực hiện cải tiến quy trình lâm sàng tại HealthSystems Minnesota, một hệ thống chăm sóc được tích hợp theo chiều dọc tại Minneapolis. Mục tiêu của CIMRDP là đưa ra những cải tiến bền vững trong việc quản lý bệnh mãn tính. Các loại nỗ lực cải thiện chất lượng này đòi hỏi sự thay đổi cơ bản ở cấp độ tổ chức, điều này thường không xảy ra và các chương trình không tiếp tục sau giai đoạn đầu. Sử dụng CIMRDP làm mô hình cho một chương trình quản lý bệnh mãn tính bền vững, bài báo này khám phá quá trình triển khai các hướng dẫn lâm sàng để quản lý bệnh mãn tính và giải quyết vấn đề phức tạp về việc khởi xướng thay đổi ở cấp độ tổ chức. Nó xem xét các yếu tố quan trọng đối với sự thành công của một chương trình như vậy, bao gồm việc sử dụng các nhóm tập trung để đảm bảo cách tiếp cận lấy bệnh nhân làm trung tâm, lợi thế của việc thuê một cố vấn bên ngoài, câu hỏi có chấp nhận nguồn tài trợ bên ngoài hay không và phác thảo đánh giá sáu nhiệm vụ chính mà chương trình yêu cầu. Các phương pháp tiếp thị và tích hợp cũng được trình bày."} +{"idx": 1950029, "en": "Both behavioral and electrophysiological methods were used to assess altitudinal neglect. In the first experiment, 100 patients with neglect completed Albert's Barrage test. Most omissions were present in the lower left quadrant. In 16 patients, visual evoked potentials to stimuli in the four quadrants were separately recorded (Exp. 2). Latencies in the lower left quadrant were longer than those in the other quadrants. A third experiment provided electrophysiological normative data from 13 young normal subjects. Overall, the results showed that both the horizontal and vertical dimensions of space are affected in neglect patients.", "gemini": "Cả các phương pháp hành vi và điện sinh lý đều được sử dụng để đánh giá sự bỏ bê về độ cao. Trong thí nghiệm đầu tiên, 100 bệnh nhân bị bỏ bê đã hoàn thành bài kiểm tra Albert's Barrage. Hầu hết các trường hợp bỏ sót đều có ở góc phần tư dưới bên trái. Ở 16 bệnh nhân, các điện thế gây ra thị giác đối với các kích thích ở bốn góc phần tư được ghi riêng biệt (Exp. 2). Độ trễ ở góc phần tư dưới bên trái dài hơn ở các góc phần tư khác. Thí nghiệm thứ ba cung cấp dữ liệu chuẩn điện sinh lý từ 13 đối tượng bình thường trẻ tuổi. Nhìn chung, kết quả cho thấy cả chiều ngang và chiều dọc của không gian đều bị ảnh hưởng ở những bệnh nhân bị bỏ bê."} +{"idx": 1950030, "en": "Normal subjects were presented with dichotic pairs of sounds differing in pitch-timbre combination. Their task was to detect a fixed target sound that occurred randomly but equally often in the right and the left ear in two-thirds of the trials. Half the subjects performed this task with sounds produced by familiar natural instruments (violin, flute, guitar and drum), and the other subjects performed the same task but with the sounds played backwards, hence being less recognizable. Subjects were quicker and more accurate in discriminating forwards than backwards played sounds, hence exhibiting sensitivity to familiarity with musical instrument sounds. Recourse to this knowledge was, however, not associated to a shift in laterality. For both familiar and unfamiliar sounds, a robust left-ear advantage (LEA) was observed.", "gemini": "Những đối tượng bình thường được trình bày với các cặp âm thanh dichotic khác nhau về sự kết hợp cao độ-âm sắc. Nhiệm vụ của họ là phát hiện ra một âm thanh mục tiêu cố định xuất hiện ngẫu nhiên nhưng thường xuyên ở tai phải và tai trái trong hai phần ba số lần thử nghiệm. Một nửa số đối tượng thực hiện nhiệm vụ này với âm thanh do các nhạc cụ tự nhiên quen thuộc tạo ra (vĩ cầm, sáo, guitar và trống), và những đối tượng còn lại thực hiện cùng nhiệm vụ nhưng với âm thanh được phát ngược, do đó ít nhận ra hơn. Đối tượng nhanh hơn và chính xác hơn trong việc phân biệt âm thanh phát về phía trước so với âm thanh phát ngược, do đó thể hiện sự nhạy cảm với sự quen thuộc với âm thanh của nhạc cụ. Tuy nhiên, việc sử dụng kiến thức này không liên quan đến sự thay đổi về độ lệch bên. Đối với cả âm thanh quen thuộc và không quen thuộc, người ta quan sát thấy một lợi thế rõ ràng của tai trái (LEA)."} +{"idx": 1950031, "en": "The paper presents a single-case study of patient J.G. showing severe calculation problems (and also agraphia, finger agnosia, right-left disorientation and apraxia) after the surgery of a left parietal tumor. Although the patient completely lost conceptual knowledge of arithmetic, she preserved part of memorised fact knowledge (multiplications and some additions and subtractions). The study indicates that arithmetic facts can be represented at a superficial level without understanding of the operation performed. The study completes a valid double dissociation between two types of knowledge involved in calculation (memorised facts and conceptual knowledge).", "gemini": "Bài báo trình bày một nghiên cứu trường hợp duy nhất về bệnh nhân J.G. cho thấy các vấn đề tính toán nghiêm trọng (và cũng là chứng mất khả năng viết, mất nhận thức ngón tay, mất phương hướng phải-trái và mất khả năng vận động) sau khi phẫu thuật khối u đỉnh trái. Mặc dù bệnh nhân hoàn toàn mất kiến thức khái niệm về số học, nhưng cô ấy vẫn giữ được một phần kiến thức về sự kiện đã ghi nhớ (phép nhân và một số phép cộng và trừ). Nghiên cứu chỉ ra rằng các sự kiện số học có thể được biểu diễn ở cấp độ hời hợt mà không hiểu về phép tính được thực hiện. Nghiên cứu hoàn thành sự phân ly kép hợp lệ giữa hai loại kiến thức liên quan đến tính toán (sự kiện được ghi nhớ và kiến thức khái niệm)."} +{"idx": 1950032, "en": "This case study presents a 37 year old man, P.P., who shows evidence of both profound executive impairment and diencephalic amnesia associated with two discrete lesion sites following a Sub Arachnoid Haemorrhage. The executive impairment is unusual in type and severity, amounting to a gross impairment in the initiation and organisation of action. The thalamic lesion provides a rare example of diencephalic damage resulting in an extensive retrograde amnesia of the type that is often associated with Korsakoff's syndrome, but which in this case is not associated with prolonged alcohol abuse. Some of the problems caused by the co-occurrence of two such profound and disabling impairments are discussed.", "gemini": "Nghiên cứu trường hợp này trình bày một người đàn ông 37 tuổi, P.P., người biểu hiện bằng chứng về cả suy giảm chức năng điều hành sâu sắc và chứng mất trí nhớ vùng dưới đồi liên quan đến hai vị trí tổn thương riêng biệt sau xuất huyết dưới nhện. Suy giảm chức năng điều hành là bất thường về loại và mức độ nghiêm trọng, tương đương với sự suy giảm nghiêm trọng trong việc khởi xướng và tổ chức hành động. Tổn thương vùng đồi thị cung cấp một ví dụ hiếm hoi về tổn thương vùng dưới đồi dẫn đến chứng mất trí nhớ ngược dòng rộng rãi, loại chứng mất trí nhớ thường liên quan đến hội chứng Korsakoff, nhưng trong trường hợp này không liên quan đến tình trạng lạm dụng rượu kéo dài. Một số vấn đề do sự xuất hiện đồng thời của hai khiếm khuyết sâu sắc và gây tàn tật như vậy được thảo luận."} +{"idx": 1950033, "en": "In bacterial meningitis, forced deviation of the head and/or the eyes towards either the left or the right side reflects an ipsilateral focal inflammation of the brain. Also, the symptom of forced deviation is associated with a high fatality and sequelae rate. In this study it was observed that, among 2124 Dutch patients with bacterial meningitis, forced deviation was significantly more often to the right than to the left. Because the right hemisphere is dominant for motor functions in natural lefthanders, it was concluded that bacterial meningitis might have been a selection mechanism favouring the evolution of a dominance of righthandedness.", "gemini": "Trong viêm màng não do vi khuẩn, hiện tượng đầu và/hoặc mắt bị lệch về một bên trái hoặc phải phản ánh tình trạng viêm cục bộ ở cùng bên của não. Ngoài ra, triệu chứng lệch hướng cưỡng bức có liên quan đến tỷ lệ tử vong và di chứng cao. Trong nghiên cứu này, người ta quan sát thấy rằng, trong số 2124 bệnh nhân Hà Lan mắc viêm màng não do vi khuẩn, hiện tượng lệch hướng cưỡng bức thường xảy ra ở bên phải nhiều hơn bên trái. Vì bán cầu não phải là bán cầu chi phối các chức năng vận động ở những người thuận tay trái tự nhiên, nên có thể kết luận rằng viêm màng não do vi khuẩn có thể là cơ chế chọn lọc ủng hộ sự tiến hóa của sự thuận tay phải."} +{"idx": 1950034, "en": "A 58 year old patient (ES) suffered severe anterograde and retrograde amnesia following prolonged cardiac arrest with presumed hypoxic brain damage. Personally relevant autobiographical memory was severely impaired as was knowledge of public events. In contrast, knowledge of famous people was very well preserved. This findings has implications for the organisation of remote memory. Knowledge of people is clearly represented independently from autobiographical memory. We argue that this pattern of profound autobiographical amnesia may result from either multifocal neocortical damage (as in this case) or a failure of the \"thematic framework\" system involved in the active reconstruction of autobiographical memory, as hypothesised in another patient with thalamic infarction who showed a similar pattern of results (Hodges and McCarthy, 1993).", "gemini": "Một bệnh nhân 58 tuổi (ES) bị mất trí nhớ tiến triển và hồi tưởng nghiêm trọng sau khi bị ngừng tim kéo dài với chẩn đoán tổn thương não do thiếu oxy. Trí nhớ tự truyện có liên quan đến cá nhân bị suy giảm nghiêm trọng cũng như kiến thức về các sự kiện công cộng. Ngược lại, kiến thức về những người nổi tiếng được bảo tồn rất tốt. Những phát hiện này có ý nghĩa đối với việc tổ chức trí nhớ từ xa. Rõ ràng kiến thức về con người được biểu diễn độc lập với trí nhớ tự truyện. Chúng tôi cho rằng kiểu mất trí nhớ tự truyện sâu sắc này có thể là do tổn thương đa ổ ở vỏ não (như trong trường hợp này) hoặc do hệ thống \"khung chủ đề\" tham gia vào quá trình tái tạo tích cực trí nhớ tự truyện bị hỏng, như được giả thuyết ở một bệnh nhân khác bị nhồi máu vùng đồi thị cũng biểu hiện kiểu kết quả tương tự (Hodges và McCarthy, 1993)."} +{"idx": 1950035, "en": "A confabulating patient MM is described who, despite clear evidence of a dysexecutive syndrome, showed normal prospective and retrospective memory in everyday life and preserved autobiographical memory. He also performed well on many, but not all laboratory-based measures of learning and memory that were given. His confabulation typically involved going well-beyond the information he could genuinely recall, and was attributed to a defect in memory monitoring resulting from his frontal lobe damage. Implications for the role of \"stop rules\" in memory retrieval are discussed.", "gemini": "Mô tả một bệnh nhân bịa đặt MM, mặc dù có bằng chứng rõ ràng về hội chứng rối loạn chức năng điều hành, nhưng vẫn biểu hiện trí nhớ hướng tới tương lai và hồi tưởng bình thường trong cuộc sống hàng ngày và bảo tồn trí nhớ tự truyện. Ông cũng thực hiện tốt nhiều biện pháp đo lường khả năng học tập và trí nhớ dựa trên phòng thí nghiệm, nhưng không phải tất cả. Sự bịa đặt của ông thường liên quan đến việc vượt xa thông tin mà ông có thể thực sự nhớ lại, và được cho là do khiếm khuyết trong quá trình theo dõi trí nhớ do tổn thương thùy trán. Các tác động đối với vai trò của \"quy tắc dừng\" trong quá trình truy xuất trí nhớ được thảo luận."} +{"idx": 1950036, "en": "We report a patient (Y.Y.) with senile dementia of the Alzheimer's type. The patient read aloud some words composed of two or three kanji characters with errors applying typical pronunciations of each character and defined them in keeping with the mispronunciation if the pronunciation represented another real word. The results of single-word semantic priming in a lexical decision task, however, suggested normal recognition processes for these kanji-words, despite the patient's reading errors. We consider her reading to represent surface dyslexia in Japanese. We discuss these dissociations between reading responses and priming effects in the context of dual-route models of human reading, and conclude that the \"visual route\" (direct access from orthography to semantics and phonology) was not completely disrupted, but partially preserved and functioning in automatic access.", "gemini": "Chúng tôi báo cáo một bệnh nhân (Y.Y.) mắc chứng mất trí nhớ do bệnh Alzheimer. Bệnh nhân đọc to một số từ gồm hai hoặc ba ký tự kanji với lỗi khi áp dụng cách phát âm thông thường của từng ký tự và định nghĩa chúng theo cách phát âm sai nếu cách phát âm đó biểu thị một từ thực khác. Tuy nhiên, kết quả của việc chuẩn bị ngữ nghĩa từ đơn trong nhiệm vụ quyết định từ vựng cho thấy các quá trình nhận dạng bình thường đối với các từ kanji này, mặc dù bệnh nhân đọc sai. Chúng tôi coi việc đọc của cô ấy đại diện cho chứng khó đọc bề mặt ở tiếng Nhật. Chúng tôi thảo luận về những sự phân ly này giữa các phản ứng đọc và các hiệu ứng chuẩn bị trong bối cảnh các mô hình đọc kép của con người và kết luận rằng \"con đường thị giác\" (truy cập trực tiếp từ chính tả đến ngữ nghĩa và ngữ âm) không bị phá vỡ hoàn toàn, nhưng được bảo tồn một phần và hoạt động trong truy cập tự động."} +{"idx": 1950037, "en": "Platelet activation is central to the pathogenesis of hemostasis and arterial thrombosis. Platelet aggregation plays a major role in acute coronary artery diseases, myocardial infarction, unstable angina, and stroke. ADP is the first known and an important agonist for platelet aggregation. ADP not only causes primary aggregation of platelets but is also responsible for the secondary aggregation induced by ADP and other agonists. ADP also induces platelet shape change, secretion from storage granules, influx and intracellular mobilization of Ca2+, and inhibition of stimulated adenylyl cyclase activity. The ADP-receptor protein mediating ADP-induced platelet responses has neither been purified nor cloned. Therefore, signal transduction mechanisms underlying ADP-induced platelet responses either remain uncertain or less well understood. Recent contributions from chemists, biochemists, cell biologists, pharmacologists, molecular biologists, and clinical investigators have added considerably to and enhanced our knowledge of ADP-induced platelet responses. Although considerable efforts have been directed toward identifying and cloning the ADP-receptor, these have not been completely successful or without controversy. Considerable progress has been made toward understanding the mechanisms of ADP-induced platelet responses but disagreements persist. New drugs that do not mimic ADP have been found to inhibit fairly selectively ADP-induced platelet activation ex vivo. Drugs that mimic ADP and selectively act at the platelet ADP-receptor have been designed, synthesized, and evaluated for their therapeutic efficacy to block selectively ADP-induced platelet responses. This review examines in detail the developments that have taken place to identify the ADP-receptor protein and to better understand mechanisms underlying ADP-induced platelet responses to develop strategies for designing innovative drugs that block ADP-induced platelet responses by acting selectively at the ADP-receptor and/or by selectively interfering with components of ADP-induced platelet activation mechanisms.", "gemini": "Hoạt hóa tiểu cầu là trung tâm của quá trình sinh bệnh của quá trình cầm máu và huyết khối động mạch. Kết tập tiểu cầu đóng vai trò chính trong các bệnh động mạch vành cấp tính, nhồi máu cơ tim, đau thắt ngực không ổn định và đột quỵ. ADP là chất chủ vận đầu tiên được biết đến và quan trọng đối với quá trình kết tập tiểu cầu. ADP không chỉ gây ra sự kết tập tiểu cầu ban đầu mà còn chịu trách nhiệm cho sự kết tập thứ cấp do ADP và các chất chủ vận khác gây ra. ADP cũng gây ra sự thay đổi hình dạng tiểu cầu, tiết ra từ các hạt dự trữ, dòng vào và huy động nội bào Ca2+ và ức chế hoạt động adenyl cyclase được kích thích. Protein thụ thể ADP trung gian cho các phản ứng tiểu cầu do ADP gây ra vẫn chưa được tinh chế hoặc nhân bản. Do đó, các cơ chế truyền tín hiệu cơ bản cho các phản ứng tiểu cầu do ADP gây ra vẫn chưa chắc chắn hoặc ít được hiểu rõ. Những đóng góp gần đây từ các nhà hóa học, nhà sinh hóa học, nhà sinh vật học tế bào, nhà dược lý học, nhà sinh học phân tử và các nhà điều tra lâm sàng đã bổ sung đáng kể và nâng cao kiến thức của chúng ta về các phản ứng tiểu cầu do ADP gây ra. Mặc dù đã có những nỗ lực đáng kể hướng tới việc xác định và nhân bản thụ thể ADP, nhưng những nỗ lực này vẫn chưa hoàn toàn thành công hoặc không gây tranh cãi. Tiến bộ đáng kể đã đạt được trong việc hiểu các cơ chế của các phản ứng tiểu cầu do ADP gây ra nhưng vẫn còn những bất đồng. Người ta đã tìm thấy các loại thuốc mới không bắt chước ADP có tác dụng ức chế khá chọn lọc hoạt hóa tiểu cầu do ADP gây ra ở ngoại bào. Các loại thuốc bắt chước ADP và có tác dụng chọn lọc tại thụ thể ADP của tiểu cầu đã được thiết kế, tổng hợp và đánh giá về hiệu quả điều trị của chúng để ngăn chặn có chọn lọc các phản ứng tiểu cầu do ADP gây ra. Bài đánh giá này xem xét chi tiết các diễn biến đã diễn ra để xác định protein thụ thể ADP và hiểu rõ hơn các cơ chế cơ bản cho các phản ứng tiểu cầu do ADP gây ra nhằm phát triển các chiến lược thiết kế thuốc sáng tạo ngăn chặn các phản ứng tiểu cầu do ADP gây ra bằng cách tác động chọn lọc vào thụ thể ADP và/hoặc bằng cách can thiệp chọn lọc vào các thành phần của cơ chế hoạt hóa tiểu cầu do ADP gây ra."} +{"idx": 1950038, "en": "DNA replication is a complicated process that is largely regulated during stages of initiation. The Siman Virus 40 in vitro replication system has served as an excellent model for studies of the initiation of DNA replication, and its regulation, in eukaryotes. Initiation of SV40 replication requires a single viral protein termed T-antigen, all other proteins are supplied by the host. The recent determination of the solution structure of the T-antigen domain that recognizes the SV40 origin has provided significant insights into the initiation process. For example, it has afforded a clearer understanding of origin recognition, T-antigen oligomerization, and DNA unwinding. Furthermore, the Simian virus 40 in vitro replication system has been used to study nascent DNA formation in the vicinity of the viral origin of replication. Among the conclusions drawn from these experiments is that nascent DNA synthesis does not initiate in the core origin in vitro and that Okazaki fragment formation is complex. These and related studies demonstrate that significant progress has been made in understanding the initiation of DNA synthesis at the molecular level.", "gemini": "Nhân đôi DNA là một quá trình phức tạp được điều chỉnh phần lớn trong các giai đoạn khởi đầu. Hệ thống nhân đôi Siman Virus 40 trong ống nghiệm đã đóng vai trò là một mô hình tuyệt vời cho các nghiên cứu về quá trình khởi đầu nhân đôi DNA và sự điều chỉnh của nó ở eukaryote. Khởi đầu quá trình nhân đôi SV40 đòi hỏi một protein virus đơn được gọi là T-antigen, tất cả các protein khác đều do vật chủ cung cấp. Việc xác định gần đây cấu trúc dung dịch của miền T-antigen nhận ra nguồn gốc SV40 đã cung cấp những hiểu biết đáng kể về quá trình khởi đầu. Ví dụ, nó đã giúp hiểu rõ hơn về sự nhận dạng nguồn gốc, quá trình oligome hóa T-antigen và sự tháo xoắn DNA. Hơn nữa, hệ thống nhân đôi Simian virus 40 trong ống nghiệm đã được sử dụng để nghiên cứu quá trình hình thành DNA mới trong vùng lân cận với nguồn gốc nhân đôi của virus. Trong số các kết luận rút ra từ các thí nghiệm này là quá trình tổng hợp DNA mới không bắt đầu ở nguồn gốc lõi trong ống nghiệm và quá trình hình thành đoạn Okazaki rất phức tạp. Những nghiên cứu này và các nghiên cứu liên quan chứng minh rằng đã có những tiến bộ đáng kể trong việc hiểu quá trình khởi đầu tổng hợp DNA ở cấp độ phân tử."} +{"idx": 1950039, "en": "The activity of the hippocampus is modulated by a serotonergic projection from the midbrain. Corticosteroids regulate the activity of this raphe-hippocampal system in various ways. These effects are differentially mediated via two types of central corticosteroid receptor types, the high-affinity mineralocorticoid receptor (MR), and the lower affinity glucocorticoid receptor (GR). Under physiological fluctuations of corticosteroid concentrations, predominantly MR-mediated effects suppress the activity of the raphe-hippocampal system, notably serotonin (5-HT)1A receptor-related activity: 5-HT1A receptors are down-regulated, and the cellular response to 5-HT1A receptor activation is attenuated. Transiently increased concentrations of corticosteroids, as induced by stress, result in combined occupation of both MR and GR, and allow increased activity of the raphe-hippocampal system. Stimulatory actions of corticosteroids involving GR occupation include increased responsiveness of hippocampal neurons to 5-HT1A receptor stimulation, attenuated autoinhibition of 5-HT, and a permissive effect on stress-induced increases in 5-HT release. Under (pathological) conditions of chronically elevated corticosteroid concentrations, however, serotonergic neurotransmission is impaired. Human depression is an important example of a condition of combined hypercorticism and an apparent hypoactivity of serotonergic transmission. Deficiency of brain GR function may be genetically determined or acquired by stress. It is proposed that the balance of MR/GR activation can be altered by chronic (stress-related) changes of corticosteroid concentrations, in combination with glucocorticoid feedback resistance. Such an imbalance would lead to a relative dominance of MR-mediated suppressive effects on the activity of the raphe-hippocampal system, which may be a biologically relevant aspect of depression.", "gemini": "Hoạt động của hồi hải mã được điều chỉnh bởi một sự chiếu xạ serotonergic từ não giữa. Corticosteroid điều chỉnh hoạt động của hệ thống raphe-hồi hải mã này theo nhiều cách khác nhau. Những tác động này được trung gian khác nhau thông qua hai loại thụ thể corticosteroid trung ương, thụ thể mineralocorticoid ái lực cao (MR) và thụ thể glucocorticoid ái lực thấp hơn (GR). Theo sự dao động sinh lý của nồng độ corticosteroid, chủ yếu là các tác động trung gian bởi MR ức chế hoạt động của hệ thống raphe-hồi hải mã, đặc biệt là hoạt động liên quan đến thụ thể serotonin (5-HT)1A: thụ thể 5-HT1A bị điều hòa giảm và phản ứng của tế bào đối với hoạt động của thụ thể 5-HT1A bị suy yếu. Nồng độ corticosteroid tăng thoáng qua, như do căng thẳng gây ra, dẫn đến sự chiếm đóng kết hợp của cả MR và GR, và cho phép tăng hoạt động của hệ thống raphe-hồi hải mã. Các tác động kích thích của corticosteroid liên quan đến sự chiếm đóng GR bao gồm tăng khả năng đáp ứng của các tế bào thần kinh hồi hải mã đối với kích thích thụ thể 5-HT1A, ức chế tự ức chế 5-HT và tác dụng cho phép tăng giải phóng 5-HT do căng thẳng. Tuy nhiên, trong các tình trạng (bệnh lý) có nồng độ corticosteroid tăng cao mạn tính, sự dẫn truyền thần kinh serotonergic bị suy yếu. Trầm cảm ở người là một ví dụ quan trọng về tình trạng tăng tiết corticoid kết hợp với hoạt động kém rõ ràng của sự dẫn truyền serotonergic. Sự thiếu hụt chức năng GR của não có thể được xác định về mặt di truyền hoặc mắc phải do căng thẳng. Người ta đề xuất rằng sự cân bằng của hoạt động MR/GR có thể bị thay đổi do những thay đổi mạn tính (liên quan đến căng thẳng) của nồng độ corticosteroid, kết hợp với khả năng kháng phản hồi glucocorticoid. Sự mất cân bằng như vậy sẽ dẫn đến sự thống trị tương đối của các tác dụng ức chế do MR trung gian đối với hoạt động của hệ thống raphe-hồi hải mã, có thể là một khía cạnh có liên quan về mặt sinh học của bệnh trầm cảm."} +{"idx": 1950040, "en": "Schizophrenia is a human brain disease with well-defined symptoms and a lifelong disease course, but without a current biological explanation. Several observations implicate brain glutamatergic abnormalities in the pathophysiology of this illness. This evidence includes both human neurochemical and clinical pharmacologic data. Furthermore the psychotomimetic action of phencyclidine, the noncompetitive NMDA-sensitive glutamate receptor antagonist, suggests the association between human psychosis and NMDA receptor blockade. This paper reviews basic aspects of glutamatergic transmission in animal and human brain with particular attention to its putative role in schizophrenia. Consideration is given to other glutamate-related human brain diseases and their purported mechanisms. Evidence of glutamatergic abnormalities in schizophrenia is critically reviewed, including data using postmortem neurochemistry, in vivo human brain imaging, clinical pharmacology, and animal models. The current theoretical formulations based on these studies are articulated. We propose a \"working\" glutamate hypothesis of schizophrenia which postulates a diminished glutamatergic transmission in the hippocampal glutamate-mediated efferent pathways and cerebral dysfunction in the hippocampus and its target areas, especially the anterior cingulate cortex. Considerable work remains to be done in this area to formulate and test a comprehensive hypothesis.", "gemini": "Tâm thần phân liệt là một bệnh về não ở người có các triệu chứng được xác định rõ ràng và diễn biến suốt đời, nhưng hiện tại vẫn chưa có lời giải thích về mặt sinh học. Một số quan sát cho thấy những bất thường về glutamat ở não trong bệnh lý sinh của căn bệnh này. Bằng chứng này bao gồm cả dữ liệu dược lý lâm sàng và hóa thần kinh ở người. Hơn nữa, tác dụng gây loạn thần của phencyclidine, chất đối kháng thụ thể glutamate NMDA không cạnh tranh, cho thấy mối liên hệ giữa loạn thần ở người và sự phong tỏa thụ thể NMDA. Bài báo này xem xét các khía cạnh cơ bản của sự dẫn truyền glutamat ở não động vật và não người, đặc biệt chú ý đến vai trò được cho là của nó trong bệnh tâm thần phân liệt. Cần xem xét các bệnh não ở người liên quan đến glutamate khác và các cơ chế được cho là của chúng. Bằng chứng về những bất thường về glutamat trong bệnh tâm thần phân liệt được xem xét một cách phê phán, bao gồm dữ liệu sử dụng hóa thần kinh sau tử thi, hình ảnh não người in vivo, dược lý lâm sàng và mô hình động vật. Các công thức lý thuyết hiện tại dựa trên các nghiên cứu này được nêu rõ. Chúng tôi đề xuất một giả thuyết glutamate \"hoạt động\" về bệnh tâm thần phân liệt, giả thuyết này đưa ra rằng sự dẫn truyền glutamat giảm trong các đường dẫn xuất trung gian glutamate của hồi hải mã và rối loạn chức năng não ở hồi hải mã và các vùng đích của nó, đặc biệt là vỏ não vành trước. Vẫn còn rất nhiều việc phải làm trong lĩnh vực này để xây dựng và kiểm tra một giả thuyết toàn diện."} +{"idx": 1950041, "en": "Multiple lines of research have implicated the mesolimbic dopamine system in drug reward measured by either the drug self-administration or conditioned place preference paradigm. The present review summarizes recent work that examines the neuropharmacological mechanisms by which drugs impinge on this dopaminergic neural circuitry, as well as other systems that provide input and output circuits to the mesolimbic dopamine system. Studies examining the effect of selective agonist and antagonist drugs administered systemically have indicated that multiple neurotransmitters are involved, including dopamine, serotonin, acetylcholine, glutamate, GABA, and various peptides. Direct microinjection studies have also provided crucial evidence indicating that, in addition to the mesolimbic dopamine system, other structures play a role in drug reward, including the ventral pallidum, amygdala, hippocampus, hypothalamus, and pedunculopontine tegmental nucleus. GABAergic circuitry descending from the nucleus accumbens to the pedunculopontine tegmental nucleus via the ventral pallidum appears to be especially important in directing the behavioral sequelae associated with reward produced by various drugs of abuse. However, activation of the reward circuitry is achieved differently for various drugs of abuse. With amphetamine and cocaine, initiation of reward is controlled within the nucleus accumbens and prefrontal cortex, respectively. With opiates, initiation of reward involves the ventral tegmental area, nucleus accumbens, hippocampus, and hypothalamus. It is not clear presently if these multiple anatomical structures mediate opiate reward by converging on a single output system or multiple output systems.", "gemini": "Nhiều hướng nghiên cứu khác nhau đã chỉ ra hệ thống dopamine mesolimbic trong phần thưởng của thuốc được đo bằng cách tự dùng thuốc hoặc mô hình sở thích nơi có điều kiện. Bản tổng quan hiện tại tóm tắt các công trình gần đây kiểm tra các cơ chế dược lý thần kinh mà thuốc tác động lên mạch thần kinh dopaminergic này, cũng như các hệ thống khác cung cấp các mạch đầu vào và đầu ra cho hệ thống dopamine mesolimbic. Các nghiên cứu kiểm tra tác dụng của thuốc chủ vận và đối kháng chọn lọc được dùng toàn thân đã chỉ ra rằng nhiều chất dẫn truyền thần kinh có liên quan, bao gồm dopamine, serotonin, acetylcholine, glutamate, GABA và nhiều peptide khác nhau. Các nghiên cứu vi tiêm trực tiếp cũng cung cấp bằng chứng quan trọng chỉ ra rằng, ngoài hệ thống dopamine mesolimbic, các cấu trúc khác cũng đóng vai trò trong phần thưởng của thuốc, bao gồm hồi xanh bụng, hạnh nhân, hồi hải mã, vùng dưới đồi và nhân tegmental pedunculopontine. Mạch thần kinh GABAergic đi xuống từ nhân accumbens đến nhân tegmental pedunculopontine qua hồi xanh bụng dường như đặc biệt quan trọng trong việc định hướng các hậu quả về hành vi liên quan đến phần thưởng do nhiều loại thuốc gây nghiện tạo ra. Tuy nhiên, việc kích hoạt mạch thần kinh phần thưởng đạt được khác nhau đối với các loại thuốc gây nghiện khác nhau. Đối với amphetamine và cocaine, việc khởi phát phần thưởng được kiểm soát tương ứng trong nhân accumbens và vỏ não trước trán. Đối với thuốc phiện, việc khởi phát phần thưởng liên quan đến vùng tegmental bụng, nhân accumbens, hồi hải mã và vùng dưới đồi. Hiện tại vẫn chưa rõ liệu những cấu trúc giải phẫu đa dạng này có trung gian phần thưởng thuốc phiện bằng cách hội tụ vào một hệ thống đầu ra duy nhất hay nhiều hệ thống đầu ra hay không."} +{"idx": 1950042, "en": "Clinical intervention in neurological disorders is almost invariably achieved using chemical agents that act on neuromediator-related sites, suggesting that intercellular chemical signaling plays a major role in determining the properties of neural networks. A variety of microvoltammetric sensors and techniques have been developed over the last 25 years to study neuromediators in intact brain in vivo, and in isolated tissues, for animal models of behavior and disease. This review, with over 600 citations, considers the advantages and limitations of the different approaches, including progress in biosensor design, illustrated by studies on the neurochemical bases of a wide variety of behaviors.", "gemini": "Can thiệp lâm sàng trong các rối loạn thần kinh hầu như luôn đạt được bằng cách sử dụng các tác nhân hóa học tác động lên các vị trí liên quan đến chất dẫn truyền thần kinh, cho thấy tín hiệu hóa học giữa các tế bào đóng một vai trò chính trong việc xác định các đặc tính của mạng lưới thần kinh. Nhiều loại cảm biến và kỹ thuật vi vôn kế đã được phát triển trong 25 năm qua để nghiên cứu các chất dẫn truyền thần kinh trong não nguyên vẹn ở trạng thái sống và trong các mô riêng biệt, đối với các mô hình động vật về hành vi và bệnh tật. Đánh giá này, với hơn 600 trích dẫn, xem xét những ưu điểm và hạn chế của các phương pháp tiếp cận khác nhau, bao gồm tiến trình thiết kế cảm biến sinh học, được minh họa bằng các nghiên cứu về cơ sở hóa học thần kinh của nhiều loại hành vi."} +{"idx": 1950043, "en": "This review examines the consequences of social deprivation on brain chemistry and behavior on rats. Although social deprivation produces wide-ranging behavioral and neurochemical effects, it appears that these effects are determined by a number of factors, the most critical factor being the age or developmental stage during the period of deprivation. Roughly, the effects examined in this review may be separated into three major developmental stages and each is related to deprivation of specific types of social interaction: preweaning/neonatal, postweaning/adolescent, and adult. The effects of social deprivation during each of these stages appears to be neurochemically and behaviorally specific. However, much of the research to date has failed to examine deprivation during specific stages, often combining deprivation of different types. Nonetheless, these modifications of experience produce animals of differing phenotypes, which could be characterized as pathological in nature in many instances, and may model particular aspects of human psychopathologies or perhaps the propensity to develop those phenotypic features.", "gemini": "Đánh giá này xem xét hậu quả của việc tước đoạt xã hội đối với hóa học não và hành vi ở chuột. Mặc dù việc tước đoạt xã hội tạo ra những tác động rộng rãi về hành vi và hóa học thần kinh, nhưng có vẻ như những tác động này được xác định bởi một số yếu tố, yếu tố quan trọng nhất là tuổi hoặc giai đoạn phát triển trong thời kỳ bị tước đoạt. Nói chung, các tác động được xem xét trong đánh giá này có thể được chia thành ba giai đoạn phát triển chính và mỗi giai đoạn liên quan đến việc tước đoạt các loại tương tác xã hội cụ thể: trước khi cai sữa/sơ sinh, sau khi cai sữa/vị thành niên và người lớn. Các tác động của việc tước đoạt xã hội trong mỗi giai đoạn này có vẻ như là đặc hiệu về mặt hóa học thần kinh và hành vi. Tuy nhiên, phần lớn các nghiên cứu cho đến nay đã không xem xét việc tước đoạt trong các giai đoạn cụ thể, thường kết hợp việc tước đoạt các loại khác nhau. Tuy nhiên, những sửa đổi về kinh nghiệm này tạo ra những con vật có kiểu hình khác nhau, trong nhiều trường hợp có thể được đặc trưng là bệnh lý về bản chất, và có thể mô hình hóa các khía cạnh cụ thể của bệnh lý tâm thần ở người hoặc có lẽ là khuynh hướng phát triển các đặc điểm kiểu hình đó."} +{"idx": 1950044, "en": "The magnitudes of cerebral somatosensory evoked potentials (SEPs), following stimulation of cutaneous or muscle afferents in the upper limb, are reduced during active and passive movements of the fingers. The generalizability of such a movement effect was tested for lower limb events. We measured SEP magnitudes following activation of cutaneous (sural) and mixed (tibial) nerves during the flexion phase of active and passive rhythmic movements of the human lower limb. In eight volunteers, 150 SEPs per condition were recorded from Cz' referenced to Fpz'. Compared to stationary controls, both active and passive movements significantly depressed the early SEP components (P1-N1) [mean values, to 12.8%, 9.9% respectively for tibial nerve and to 29.6%, 25.6% for sural nerve stimulation, p < 0.05]. The attenuation was still observed when only one leg was moved and with stimulation at an earlier point in the flexion phase of movement. Visual fixation did not significantly affect P1-N1 amplitudes, compared to eyes closed. As previously shown, soleus H reflexes with stable M waves were significantly depressed during the movements (p < 0.05). The general construct may be that centripetal flow initiated from somatosensory receptors during limb movement leads to modulation of both spinal and cortical responses following large diameter cutaneous or muscle afferent activation.", "gemini": "Độ lớn của tiềm năng gợi ra cảm giác cơ thể ở não (SEP), sau khi kích thích các sợi hướng tâm trên da hoặc cơ ở chi trên, giảm trong quá trình vận động chủ động và thụ động của các ngón tay. Tính tổng quát của hiệu ứng vận động như vậy đã được kiểm tra đối với các sự kiện ở chi dưới. Chúng tôi đã đo độ lớn SEP sau khi kích hoạt các dây thần kinh trên da (sural) và hỗn hợp (mắt cá chân) trong giai đoạn gấp của các chuyển động nhịp nhàng chủ động và thụ động của chi dưới ở người. Ở tám tình nguyện viên, 150 SEP trên mỗi tình trạng đã được ghi lại từ Cz' được tham chiếu đến Fpz'. So với các đối chứng đứng yên, cả chuyển động chủ động và thụ động đều làm giảm đáng kể các thành phần SEP sớm (P1-N1) [giá trị trung bình, lần lượt là 12,8%, 9,9% đối với dây thần kinh chày và 29,6%, 25,6% đối với kích thích dây thần kinh sural, p < 0,05]. Sự suy giảm vẫn được quan sát thấy khi chỉ một chân được di chuyển và khi kích thích tại một điểm sớm hơn trong giai đoạn gấp của chuyển động. Sự cố định bằng mắt không ảnh hưởng đáng kể đến biên độ P1-N1, so với việc nhắm mắt. Như đã trình bày trước đây, phản xạ H của cơ soleus với sóng M ổn định đã giảm đáng kể trong quá trình vận động (p < 0,05). Cấu trúc chung có thể là dòng hướng tâm bắt đầu từ các thụ thể cảm giác cơ thể trong quá trình vận động chi dẫn đến sự điều chế cả phản ứng tủy sống và vỏ não sau khi kích hoạt các sợi hướng tâm cơ hoặc da có đường kính lớn."} +{"idx": 1950045, "en": "The effects of rubber factory environment on functional integrity of auditory pathway have been studied in forty rubber factory workers using Brainstem Auditory Evoked Potentials (BAEPs) technique to detect early subclinical impairments. Results indicate that 47 percent of the workers showed abnormalities in prolongations of either peak latencies or interpeak latencies when compared with age and sex matched control subjects not exposed to rubber factory environment. The percent distribution of abnormalities (ears affected) were in the order of extrusion and calendering (75%) > vulcanising (41.66%) > mixing (28.57%) > loading and dispatch (23.07%) > tubing (18.75%) sections of the factory. This incidence of abnormalities may be attributed to solvents being used in these units of rubber factory. These findings suggest that rubber factory environment does affect auditory pathway in the brainstem.", "gemini": "Ảnh hưởng của môi trường nhà máy cao su đến tính toàn vẹn chức năng của đường dẫn thính giác đã được nghiên cứu ở bốn mươi công nhân nhà máy cao su bằng kỹ thuật tiềm năng thính giác thân não (BAEP) để phát hiện những suy giảm cận lâm sàng sớm. Kết quả cho thấy 47 phần trăm công nhân cho thấy những bất thường trong thời gian kéo dài độ trễ đỉnh hoặc độ trễ giữa các đỉnh khi so sánh với các đối tượng kiểm soát phù hợp về tuổi và giới tính không tiếp xúc với môi trường nhà máy cao su. Tỷ lệ phân bố bất thường (tai bị ảnh hưởng) theo thứ tự là ép đùn và cán (75%)> lưu hóa (41,66%)> trộn (28,57%)> nạp và vận chuyển (23,07%)> ống (18,75%) các bộ phận của nhà máy. Tỷ lệ bất thường này có thể là do dung môi được sử dụng trong các đơn vị của nhà máy cao su. Những phát hiện này cho thấy môi trường nhà máy cao su thực sự ảnh hưởng đến đường dẫn thính giác ở thân não."} +{"idx": 1950046, "en": "Over the last few years, neurologists have been showing increasing interest in the study of the sympathetic skin response (SSR). In the present report we describe a simple method that permitted us to determine a wide variation of SSR in response to different stimuli such as respiration, deglutition, blinking, skeletal movements, biting, auditory or light stimuli, vocalization, and sphincter contraction. These results raise doubts about the role of SSR as a complementary diagnostic method.", "gemini": "Trong vài năm trở lại đây, các nhà thần kinh học ngày càng quan tâm đến nghiên cứu về phản ứng da giao cảm (SSR). Trong báo cáo hiện tại, chúng tôi mô tả một phương pháp đơn giản cho phép chúng tôi xác định sự thay đổi rộng rãi của SSR để đáp ứng với các kích thích khác nhau như hô hấp, nuốt, chớp mắt, cử động xương, cắn, kích thích thính giác hoặc ánh sáng, phát âm và co thắt cơ vòng. Những kết quả này làm dấy lên nghi ngờ về vai trò của SSR như một phương pháp chẩn đoán bổ sung."} +{"idx": 1950047, "en": "To determine whether there are disturbances of hippocampal volume asymmetry in schizophrenic patients, we obtained contiguous, 1-mm-thick magnetic resonance images in 28 males with chronic schizophrenia and in 28 age-matched healthy males. The schizophrenic patients showed a bilateral reduction in volume of the hippocampal formation (HF; left 7.0%; right 8.7%). This reduction was significantly associated with the severity of disorganization syndrome (P < 0.0005). A significant asymmetry in the HF volume was found in the control subjects (P = 0.006), but not in the patients (P = 0.40). There was a significant positive correlation between the asymmetry index and the patient's age at the onset of schizophrenia (r = 0.46, P = 0.01). Results indicate that a disturbance in the normal asymmetry of the HF may be a characteristic in schizophrenia, particularly in patients with an early onset of the illness.", "gemini": "Để xác định xem có sự rối loạn về tính không đối xứng về thể tích hồi hải mã ở những bệnh nhân mắc chứng tâm thần phân liệt hay không, chúng tôi đã thu được hình ảnh cộng hưởng từ liên tiếp, dày 1 mm ở 28 nam giới mắc chứng tâm thần phân liệt mạn tính và ở 28 nam giới khỏe mạnh cùng độ tuổi. Những bệnh nhân tâm thần phân liệt cho thấy giảm thể tích song phương của hồi hải mã (HF; trái 7,0%; phải 8,7%). Sự giảm này có liên quan đáng kể đến mức độ nghiêm trọng của hội chứng mất tổ chức (P < 0,0005). Một sự không đối xứng đáng kể về thể tích HF được tìm thấy ở các đối tượng kiểm soát (P = 0,006), nhưng không có ở những bệnh nhân (P = 0,40). Có một mối tương quan dương đáng kể giữa chỉ số không đối xứng và tuổi của bệnh nhân khi khởi phát bệnh tâm thần phân liệt (r = 0,46, P = 0,01). Kết quả cho thấy rằng sự rối loạn về tính không đối xứng bình thường của HF có thể là một đặc điểm ở bệnh tâm thần phân liệt, đặc biệt ở những bệnh nhân khởi phát bệnh sớm."} +{"idx": 1950048, "en": "F responses were recorded in 6 cases with SSP and in 22 normal controls. The present study confirmed that frequency, number of phases, F/M amplitude ratio and duration were significantly increased and CV of onset latencies was significantly reduced in cases with SSP. After intravenous injection of TRH (2 mg), all the parameters were altered toward normal sides. It was suggested that the hyperexcitability of motor neuron pool in SSP was stabilized by effects of TRH on injured pyramidal tracts and consequently abnormalities of F responses were improved. The Effect of TRH to correct the abnormal F responses in SSP might be consistent with effects of TRH to reduce spasticity in amyotrophic lateral sclerosis described previously.", "gemini": "Đáp ứng F được ghi nhận ở 6 trường hợp mắc SSP và 22 đối chứng bình thường. Nghiên cứu hiện tại xác nhận rằng tần số, số pha, tỷ lệ biên độ F/M và thời gian tăng đáng kể và CV của độ trễ khởi phát giảm đáng kể ở những trường hợp mắc SSP. Sau khi tiêm tĩnh mạch TRH (2 mg), tất cả các thông số đều thay đổi theo hướng bình thường. Người ta cho rằng sự tăng kích thích của nhóm tế bào thần kinh vận động ở SSP được ổn định bởi tác dụng của TRH lên các đường tháp bị tổn thương và do đó những bất thường của đáp ứng F được cải thiện. Tác dụng của TRH trong việc điều chỉnh các đáp ứng F bất thường ở SSP có thể phù hợp với tác dụng của TRH trong việc giảm co cứng trong bệnh xơ cứng teo cơ một bên đã được mô tả trước đây."} +{"idx": 1950049, "en": "We previously described a Swedish set of male schizophrenic monozygotic triplets. In this study the patients as well as their parents were further characterized. By high-resolution chromosomal analysis an extra band at chromosome 15p was found in all the triplets and the father. Microdissection, degenerate oligonucleotide-primed PCR (DOP-PCR) amplification and reverse painting indicates that the extra band probably contains only repetitive DNA sequences with no known effect on the phenotype. Magnetic resonance imaging (MRI) showed similar borderline ventricular enlargement and widened subarachnoid spaces over frontoparietal and basal regions as well as around the pituitary gland (empty sella) in all the triplets. The father also had widened subarachnoid spaces over the frontal and basal regions. The mother had an empty sella indicating widened subarachnoid spaces. All the boys also had a right-sided conductive hearing defect, probably due to malformation and fixation of the ossicular chain. The parents did not present any otological abnormalities. Neuropsychological assessment demonstrated similar marked reductions of attentional, mnestic, and executive functions in all the triplets, but the mother showed a normal pattern. Possible joint etiological mechanisms for the psychological and somatic abnormalities recorded in the triplets are discussed.", "gemini": "Trước đây chúng tôi đã mô tả một bộ ba đơn hợp tử nam mắc bệnh tâm thần phân liệt người Thụy Điển. Trong nghiên cứu này, bệnh nhân cũng như cha mẹ của họ được mô tả thêm. Bằng cách phân tích nhiễm sắc thể có độ phân giải cao, người ta phát hiện ra một dải thêm ở nhiễm sắc thể 15p ở tất cả những bộ ba và người cha. Giải phẫu vi phẫu, khuếch đại PCR mồi oligonucleotide thoái hóa (DOP-PCR) và sơn ngược cho thấy dải thêm có thể chỉ chứa các trình tự DNA lặp lại không có tác dụng đã biết đối với kiểu hình. Chụp cộng hưởng từ (MRI) cho thấy sự giãn nở não thất ranh giới tương tự và các khoảng dưới nhện rộng hơn ở vùng trán đỉnh và vùng đáy cũng như xung quanh tuyến yên (sella trống) ở tất cả những bộ ba. Người cha cũng có khoảng dưới nhện rộng hơn ở vùng trán và vùng đáy. Người mẹ có sella trống cho thấy khoảng dưới nhện rộng hơn. Tất cả các bé trai cũng bị khiếm khuyết về thính giác dẫn truyền bên phải, có thể là do dị tật và cố định của chuỗi xương con. Cha mẹ không biểu hiện bất kỳ bất thường nào về tai. Đánh giá tâm lý thần kinh cho thấy sự giảm đáng kể tương tự về chức năng chú ý, trí nhớ và điều hành ở tất cả những bộ ba, nhưng người mẹ lại biểu hiện mô hình bình thường. Các cơ chế nguyên nhân chung có thể có đối với các bất thường về tâm lý và thể chất được ghi nhận ở những bộ ba được thảo luận."} +{"idx": 1950050, "en": "The exteroceptive reflexes in the masseter muscles in patients with Parkinson's disease (PD) were examined. The reflex produced by 3 Hz mental nerve stimulation consisted of four negative peaks and three positive peaks in the unrectified recordings. The latencies of the peaks in patients with PD were the same as those in control subjects, except for the P2 component in the right masseter muscle. The amplitudes of N3-P3 and P3-N4 were smaller in patients with PD than those in control subjects. Interpeak latency of N3-N4 was shorter in PD patients. Exteroceptive suppression in the masseter muscles was also examined. The early component (ES1) of so-called exteroceptive suppression was normal in patients with PD. The duration and degree of the late component (ES2) of exteroceptive suppression decreased in patients with PD. According to the latencies, N1 and N2 speaks seem to be the onset and end of ESI; N3 and N3 peaks the onset and end of ES2, respectively. The early component did not show any significant abnormality. Reduced late component of exteroceptive reflex in patients with PD might be related to the abnormal motor control system of the facial muscles.", "gemini": "Các phản xạ ngoại cảm ở cơ cắn ở những bệnh nhân mắc bệnh Parkinson (PD) đã được kiểm tra. Phản xạ do kích thích thần kinh tinh thần 3 Hz bao gồm bốn đỉnh âm và ba đỉnh dương trong các bản ghi không chỉnh lưu. Độ trễ của các đỉnh ở những bệnh nhân mắc PD giống với những bệnh nhân đối chứng, ngoại trừ thành phần P2 ở cơ cắn bên phải. Biên độ của N3-P3 và P3-N4 nhỏ hơn ở những bệnh nhân mắc PD so với những bệnh nhân đối chứng. Độ trễ liên đỉnh của N3-N4 ngắn hơn ở những bệnh nhân mắc PD. Sự ức chế ngoại cảm ở cơ cắn cũng được kiểm tra. Thành phần sớm (ES1) của cái gọi là ức chế ngoại cảm là bình thường ở những bệnh nhân mắc PD. Thời gian và mức độ của thành phần muộn (ES2) của sự ức chế ngoại cảm giảm ở những bệnh nhân mắc PD. Theo độ trễ, các đỉnh N1 và N2 có vẻ là điểm bắt đầu và kết thúc của ESI; Các đỉnh N3 và N3 lần lượt là điểm bắt đầu và kết thúc của ES2. Thành phần sớm không biểu hiện bất kỳ bất thường đáng kể nào. Thành phần muộn giảm của phản xạ ngoại cảm ở những bệnh nhân mắc PD có thể liên quan đến hệ thống kiểm soát vận động bất thường của các cơ mặt."} +{"idx": 1950051, "en": "Adjunct bright-light therapy has been suggested to augment antidepressant drug treatment in patients with non-seasonal major depression. Side effects of the combined therapy have not been investigated thus far. Therefore, somatic complaints and side effects of combined therapy were evaluated in 28 patients with major depression (DSM-III-R) randomly assigned to either trimipramine or trimipramine and serially applied adjunct bright-light therapy. Response rates were comparable in both treatment groups and rates of newly emergent side effects during treatment were generally low. The most prominent unfavourable side effects of adjunct bright-light therapy as compared with trimipramine monotherapy were aggravated sedation, persisting restlessness, emerging sleep disturbance and decreased appetite as well as the worsening of vertigo. Discriminant analysis revealed that the combination of trimipramine with bright light results in a different side effect profile compared with drug monotherapy.", "gemini": "Liệu pháp bổ sung bằng ánh sáng mạnh đã được đề xuất để tăng cường điều trị bằng thuốc chống trầm cảm ở những bệnh nhân bị trầm cảm nặng không theo mùa. Tác dụng phụ của liệu pháp kết hợp cho đến nay vẫn chưa được nghiên cứu. Do đó, các triệu chứng về thể chất và tác dụng phụ của liệu pháp kết hợp đã được đánh giá ở 28 bệnh nhân bị trầm cảm nặng (DSM-III-R) được chỉ định ngẫu nhiên dùng trimipramine hoặc trimipramine và liệu pháp bổ sung bằng ánh sáng mạnh theo từng đợt. Tỷ lệ đáp ứng ở cả hai nhóm điều trị là tương đương và tỷ lệ tác dụng phụ mới xuất hiện trong quá trình điều trị nói chung là thấp. Các tác dụng phụ không mong muốn nổi bật nhất của liệu pháp bổ sung bằng ánh sáng mạnh so với liệu pháp đơn trị bằng trimipramine là tình trạng an thần trầm trọng hơn, tình trạng bồn chồn kéo dài, rối loạn giấc ngủ mới xuất hiện và chán ăn cũng như tình trạng chóng mặt trở nên tồi tệ hơn. Phân tích phân biệt cho thấy sự kết hợp của trimipramine với ánh sáng mạnh dẫn đến một hồ sơ tác dụng phụ khác so với liệu pháp đơn trị bằng thuốc."} +{"idx": 1950052, "en": "Electroencephalographical studies have disclosed correlations between topographical features of Fast Fourier Transformation maps and the severity of Alzheimer's disease (DAT). The object of the present study was to explore the relations of HMPAO-SPECT and quantitative EEG (qEEG) with the severity of dementia. Twenty-three patients were included in the study. Spectral and topographical EEG parameters were compared with global and regional cerebral blood flow, and with psychometric measures of clinical severity. None of the regions of interest of the SPECT scans were significantly correlated with clinical severity. Low values in delta- and theta bands, however, were related to high scores on the Mini-Mental-State examination (P < 0.01), whereas the Syndrome-Kurz test correlated inversely with the power values in the alpha and beta band. The global decrease in cerebral blood flow (CBF) was associated with a shift on the topographical alpha-centroids in the posterior direction (P < 0.01). In previous studies correlations between CBF and clinical severity were weak, indicating a high interindividual variance, or interactions with concomitant vascular lesions. Whereas SPECT is a well-established tool for the diagnosis of dementia, the present study indicates qEEG as a potential marker for the staging of the cognitive decline in DAT.", "gemini": "Các nghiên cứu điện não đồ đã tiết lộ mối tương quan giữa các đặc điểm địa hình của bản đồ Biến đổi Fourier nhanh và mức độ nghiêm trọng của bệnh Alzheimer (DAT). Mục đích của nghiên cứu hiện tại là khám phá mối quan hệ của HMPAO-SPECT và điện não đồ định lượng (qEEG) với mức độ nghiêm trọng của chứng mất trí. Hai mươi ba bệnh nhân đã được đưa vào nghiên cứu. Các thông số EEG theo phổ và địa hình được so sánh với lưu lượng máu não toàn cầu và khu vực, và với các biện pháp trắc nghiệm tâm lý về mức độ nghiêm trọng lâm sàng. Không có vùng nào được quan tâm của các lần quét SPECT có mối tương quan đáng kể với mức độ nghiêm trọng lâm sàng. Tuy nhiên, các giá trị thấp trong băng tần delta và theta có liên quan đến điểm số cao trong bài kiểm tra Trạng thái tinh thần thu nhỏ (P < 0,01), trong khi bài kiểm tra Syndrome-Kurz có mối tương quan nghịch với các giá trị công suất trong băng tần alpha và beta. Giảm lưu lượng máu não (CBF) toàn cầu có liên quan đến sự thay đổi về hướng sau của các trọng tâm alpha theo địa hình (P < 0,01). Trong các nghiên cứu trước, mối tương quan giữa CBF và mức độ nghiêm trọng lâm sàng là yếu, cho thấy có sự khác biệt lớn giữa các cá thể hoặc tương tác với các tổn thương mạch máu đi kèm. Trong khi SPECT là một công cụ được thiết lập tốt để chẩn đoán chứng mất trí, thì nghiên cứu hiện tại chỉ ra qEEG là một dấu hiệu tiềm năng để đánh giá giai đoạn suy giảm nhận thức trong DAT."} +{"idx": 1950053, "en": "This study objective was to verify the behavior of three portions of the abdominal rectus muscle through a quantitative analysis of the electromyographic signal in different types of abdominal exercises. Ten young male between 16 and 27 years old were studied and they had no previous history of muscle and joint illness. They were well-trained and did seven abdominal exercises chosen considering the types of contraction (isotonic and isometric) as well as the muscle fixation points. The electric activity of the superior, medium (above umbilicus) and inferior (below umbilicus) portions at the left side of the abdominal rectus muscle was taken using Beckman type surface mini-electrodes. The registers were collected from computerized 8-channel Nicholet electromyography equipment, model Viking II. The signals were quantified using the MVA (Maximum Volunteer Activity) software, being considered for analysis the values of RMS (Root Mean Square). The obtained data were submitted to a parametric analysis using the variance analysis (F test) and also the Tukey test, besides a descriptive graphic analysis starting from the average RMS values of each muscle portion. This study results suggest that for the majority of the subjects, the functional activities of the abdominal rectus muscle are performed with electric activity differences among their portions, showing a tendency of producing more electric activity in the superior portion than in the medium and inferior portions. The experiment also demonstrated an absence of a common behavior pattern in the three portions of the ten tested subjects.", "gemini": "Mục tiêu của nghiên cứu này là xác minh hành vi của ba phần của cơ trực tràng bụng thông qua phân tích định lượng tín hiệu điện cơ trong các loại bài tập bụng khác nhau. Mười nam thanh niên từ 16 đến 27 tuổi đã được nghiên cứu và họ không có tiền sử mắc bệnh về cơ và khớp. Họ được đào tạo bài bản và đã thực hiện bảy bài tập bụng được lựa chọn có tính đến các loại co cơ (đẳng trương và đẳng áp) cũng như các điểm cố định cơ. Hoạt động điện của các phần trên, giữa (trên rốn) và dưới (dưới rốn) ở bên trái của cơ trực tràng bụng đã được ghi lại bằng điện cực mini bề mặt loại Beckman. Các thanh ghi được thu thập từ thiết bị điện cơ đồ Nicholet 8 kênh được vi tính hóa, kiểu Viking II. Các tín hiệu được định lượng bằng phần mềm MVA (Hoạt động tình nguyện tối đa), được coi là để phân tích các giá trị RMS (Trung bình bình phương gốc). Dữ liệu thu được đã được đưa vào phân tích tham số sử dụng phân tích phương sai (kiểm định F) và kiểm định Tukey, bên cạnh phân tích đồ họa mô tả bắt đầu từ các giá trị RMS trung bình của từng phần cơ. Kết quả nghiên cứu này cho thấy rằng đối với phần lớn các đối tượng, các hoạt động chức năng của cơ trực tràng bụng được thực hiện với sự khác biệt về hoạt động điện giữa các phần của chúng, cho thấy xu hướng tạo ra nhiều hoạt động điện hơn ở phần trên so với phần giữa và phần dưới. Thí nghiệm cũng chứng minh không có một mô hình hành vi chung ở cả ba phần của mười đối tượng được thử nghiệm."} +{"idx": 1950054, "en": "Complex motor unit potentials (CMUPs) with satellites were recorded and analysed in 231 electromyograms of patients with spinal muscular atrophy (SMA), amyotrophic lateral sclerosis (ALS), Duchenne muscular dystrophy (DMD) and a healthy control group. In the control adult group only few CMUPs with satellites were found; no CMUP were found in children. In SMA and DMD patients the CMUPs were present with similar frequency but their morphology, i.e. shape, duration, amplitude of the main and satellite spike components and number of satellites was different. In ALS patients a significant difference between severely involved interosseous muscle and proximal muscles was found. The pathomechanism of CMUPs with satellites is different in myopathic and neurogenic processes, in both however they reflect remodelling of the motor unit. In myopathy they reflect muscle fiber diameter variability and distribution of preserved muscle fibers within the motor unit under study. In neurogenic lesion the CMUPs with satellites are the result of increasing desynchronisation during progressive de- and reinnervation.", "gemini": "Tiềm năng đơn vị vận động phức hợp (CMUP) có vệ tinh được ghi lại và phân tích trong 231 điện cơ đồ của bệnh nhân teo cơ tủy sống (SMA), xơ cứng teo cơ một bên (ALS), loạn dưỡng cơ Duchenne (DMD) và nhóm đối chứng khỏe mạnh. Trong nhóm người lớn đối chứng, chỉ tìm thấy một vài CMUP có vệ tinh; không tìm thấy CMUP nào ở trẻ em. Ở bệnh nhân SMA và DMD, CMUP có mặt với tần suất tương tự nhưng hình thái của chúng, tức là hình dạng, thời gian, biên độ của các thành phần gai chính và vệ tinh và số lượng vệ tinh thì khác nhau. Ở bệnh nhân ALS, người ta thấy có sự khác biệt đáng kể giữa cơ liên cốt bị tổn thương nặng và các cơ gần. Cơ chế bệnh sinh của CMUP có vệ tinh khác nhau ở các quá trình cơ và thần kinh, nhưng ở cả hai trường hợp đều phản ánh sự tái tạo của đơn vị vận động. Ở bệnh cơ, chúng phản ánh sự thay đổi đường kính sợi cơ và sự phân bố của các sợi cơ được bảo tồn trong đơn vị vận động đang nghiên cứu. Ở tổn thương thần kinh, CMUP có vệ tinh là kết quả của quá trình mất đồng bộ ngày càng tăng trong quá trình khử và tái tạo thần kinh."} +{"idx": 1950055, "en": "Physiological (heart rate, blood pressure, electrodermal activity), biochemical (epinephrine, norepinephrine, cortisol) and subjective parameters (self-rating score) of 33 patients with panic disorder (diagnoses according to DSM-III-R) before, during and after stress exposure were compared with those of healthy controls. As stressors a video containing frightening scenes (FS), mental arithmetic (MA), a video documenting a patient suffering from a panic attack (PA) and an improvised speech (IS) were applied. We found significantly higher baseline levels of electrodermal activity (EDA) and norepinephrine (NE) secretion and a subsequent further increase during stress exposure in panic disorder patients as compared with normal controls. The most potent stressors during the trial proved to be mental arithmetics and improvised speech, which was evident in both groups. The situation panic attack video appeared to be a \"panic disorder patient-specific\" stressor; here we noticed the most pronounced reactions in the patient group. Panic disorder patients had significantly higher self-rating scores of the parameters panicky feelings, anxiety and nervousness at the beginning and throughout the investigation. We conclude that panic disorder patients have a higher degree of activation compared with normal controls, which is evident regarding levels of electrodermal activity and norepinephrine secretion. Furthermore, the panic attack video appears to be a panic disorder patient-specific stressor.", "gemini": "Các thông số sinh lý (nhịp tim, huyết áp, hoạt động điện da), sinh hóa (epinephrine, norepinephrine, cortisol) và chủ quan (điểm tự đánh giá) của 33 bệnh nhân mắc chứng rối loạn hoảng sợ (chẩn đoán theo DSM-III-R) trước, trong và sau khi tiếp xúc với căng thẳng đã được so sánh với những thông số của nhóm đối chứng khỏe mạnh. Là những tác nhân gây căng thẳng, một đoạn video có chứa những cảnh đáng sợ (FS), số học tinh thần (MA), một đoạn video ghi lại cảnh một bệnh nhân đang lên cơn hoảng sợ (PA) và một bài phát biểu ngẫu hứng (IS) đã được áp dụng. Chúng tôi thấy mức hoạt động điện da (EDA) và tiết norepinephrine (NE) cơ bản cao hơn đáng kể và sau đó tăng thêm nữa trong quá trình tiếp xúc với căng thẳng ở những bệnh nhân mắc chứng rối loạn hoảng sợ so với những người đối chứng bình thường. Các tác nhân gây căng thẳng mạnh nhất trong quá trình thử nghiệm đã được chứng minh là số học tinh thần và bài phát biểu ngẫu hứng, điều này được thấy rõ ở cả hai nhóm. Video cơn hoảng sợ dường như là một tác nhân gây căng thẳng \"dành riêng cho bệnh nhân mắc chứng rối loạn hoảng sợ\"; ở đây chúng tôi nhận thấy những phản ứng rõ rệt nhất ở nhóm bệnh nhân. Những bệnh nhân mắc chứng rối loạn hoảng sợ có điểm tự đánh giá cao hơn đáng kể về các thông số cảm giác hoảng sợ, lo lắng và hồi hộp khi bắt đầu và trong suốt quá trình điều tra. Chúng tôi kết luận rằng những bệnh nhân mắc chứng rối loạn hoảng sợ có mức độ hoạt hóa cao hơn so với những người đối chứng bình thường, điều này được thể hiện rõ ở mức độ hoạt động điện da và tiết norepinephrine. Hơn nữa, video cơn hoảng sợ dường như là một tác nhân gây căng thẳng dành riêng cho bệnh nhân mắc chứng rối loạn hoảng sợ."} +{"idx": 1950056, "en": "A cross-sectional survey was undertaken to study the aging effect on platelet cytoplasmic membrane outer leaflet fluidity and Na+/H+ antiporter activity, and to investigate the relative influence of membrane order and age on the Na+/H+ exchanger. The study population consisted of 19 healthy subjects (age range 22-83 years, mean age 48.5 +/- 16.5 years; 12 females and 7 males). Washed platelets were used as neuronal models and as eventually contributing to brain pathology by aberrant secretion of their granule content. A significant positive correlation (r = 0.48, p < 0.05) was found between age and the membrane structural order, according to a second-degree equation. Sewall-Wright path coefficient analysis revealed that the age influence on Na+/H+ exchanger activity was unimportant (0.18), but those of cytoplasmic membrane outer leaflet fluidity and mean platelet volume were strong and positive (0.51 and 0.54, respectively). Women presented a marked standard deviation in the countertransporter results. In conclusion, decreased membrane outer leaflet fluidity and unchanged Na+/H+ antiporter activity may contribute to the process of normal brain aging.", "gemini": "Một cuộc khảo sát cắt ngang đã được tiến hành để nghiên cứu tác động của lão hóa đến tính lưu động của lớp ngoài màng tế bào chất tiểu cầu và hoạt động của chất vận chuyển Na+/H+, và để điều tra ảnh hưởng tương đối của trật tự màng và tuổi tác đến chất trao đổi Na+/H+. Đối tượng nghiên cứu bao gồm 19 đối tượng khỏe mạnh (tuổi từ 22-83, tuổi trung bình 48,5 +/- 16,5 năm; 12 nữ và 7 nam). Tiểu cầu đã rửa được sử dụng làm mô hình nơ-ron và cuối cùng góp phần vào bệnh lý não bằng cách tiết bất thường nội dung hạt của chúng. Một mối tương quan dương có ý nghĩa (r = 0,48, p < 0,05) đã được tìm thấy giữa tuổi và trật tự cấu trúc màng, theo một phương trình bậc hai. Phân tích hệ số đường dẫn Sewall-Wright cho thấy ảnh hưởng của tuổi đến hoạt động trao đổi Na+/H+ không quan trọng (0,18), nhưng ảnh hưởng của tính lưu động của lớp ngoài màng tế bào chất và thể tích tiểu cầu trung bình thì mạnh và dương (lần lượt là 0,51 và 0,54). Phụ nữ có độ lệch chuẩn đáng kể trong kết quả đối vận chuyển. Tóm lại, tính lưu động của lớp ngoài màng giảm và hoạt động của chất vận chuyển Na+/H+ không thay đổi có thể góp phần vào quá trình lão hóa não bình thường."} +{"idx": 1950057, "en": "In a two-wave community study in Munich, Germany, a representative sample of 402 people older than 85 years was restudied 1 year later. In the first cross section a total of 358 (89.1%) subjects were interviewed. One year later 263 (73.5%) subjects were reexamined. Several diagnostic systems were used. The probands showed a high prevalence of depression--nearly one quarter of the interviewees. In this extreme age group gender differences in depression were minimal. Depressive were distinctly impaired in daily life. Depressive probands were more often found in institutions than in private households. Depressives were high users of the medical care system, but very rarely treated by specialists. Need for care and certain specific especially threatening life events were identified as risk factors for depression.", "gemini": "Trong một nghiên cứu cộng đồng hai đợt ở Munich, Đức, một mẫu đại diện gồm 402 người trên 85 tuổi đã được nghiên cứu lại sau 1 năm. Trong lần cắt ngang đầu tiên, tổng cộng có 358 (89,1%) đối tượng được phỏng vấn. Một năm sau, 263 (73,5%) đối tượng được tái khám. Một số hệ thống chẩn đoán đã được sử dụng. Những người được thử nghiệm cho thấy tỷ lệ trầm cảm cao - gần một phần tư số người được phỏng vấn. Ở nhóm tuổi cực cao này, sự khác biệt về giới tính trong bệnh trầm cảm là rất nhỏ. Những người bị trầm cảm rõ ràng bị suy yếu trong cuộc sống hàng ngày. Những người được thử nghiệm bị trầm cảm thường được tìm thấy ở các cơ sở hơn là ở các hộ gia đình tư nhân. Những người bị trầm cảm là người sử dụng nhiều hệ thống chăm sóc y tế, nhưng rất hiếm khi được các bác sĩ chuyên khoa điều trị. Nhu cầu chăm sóc và một số sự kiện đe dọa đến tính mạng cụ thể đã được xác định là các yếu tố nguy cơ gây trầm cảm."} +{"idx": 1950058, "en": "The present study was performed in order to re-evaluate a previous report on motor nerve conduction measurement of the intercostal nerves (IN) by electrical stimulation (ES). In addition, it was aimed to compare ES with magnetic stimulation (MS). The 7th to 9th IN were investigated in 50 healthy volunteers, aged 20 to 80 years (mean age 35 +/- 16 years). The recording electrodes were placed over the cranial part of the rectus abdominal muscle. Stimulation was performed paravertebrally, and at a distance of 10 cm between stimulating and recording electrodes. The mean distal motor latency was slightly, but significantly longer after ES than MS (2.5 +/- 0.4 ms and 2.7 +/- 0.3 ms, respectively, p < 0.01). ES and MS resulted in similar mean motor nerve conduction velocities (NCV; 77 +/- 10 m/s and 79 +/- 10 m/s, respectively; p = 0.2). The amplitudes of the compound motor action potentials (CMAP) after distal stimulation versus proximal stimulation were significantly different in ES (2.5 vs. 1.9 mV; p < 0.01) and MS (3.6 vs. 1.6 mV; p < 0.001, resp.). In one subject, neither distal nor proximal ES evoked a CMAP. In eight subjects, proximal ES failed to elicit a CMAP. In all subjects, distal MS resulted in a visible CMAP, whereas in two subjects proximal MS was unsuccessful. Thus, MS proved to be significantly more effective in eliciting a CMAP (p < 0.05). Moreover, MS was agreed to be less unpleasant than ES. In summary, MS facilitates nerve conduction studies of the IN and is superior to ES regarding performance and acceptance.", "gemini": "Nghiên cứu hiện tại được thực hiện để đánh giá lại một báo cáo trước đây về phép đo dẫn truyền thần kinh vận động của các dây thần kinh liên sườn (IN) bằng kích thích điện (ES). Ngoài ra, mục đích là để so sánh ES với kích thích từ (MS). Các IN từ thứ 7 đến thứ 9 đã được nghiên cứu ở 50 tình nguyện viên khỏe mạnh, trong độ tuổi từ 20 đến 80 (tuổi trung bình 35 +/- 16 năm). Các điện cực ghi được đặt trên phần sọ của cơ bụng trực tràng. Kích thích được thực hiện ở vị trí cạnh đốt sống, và ở khoảng cách 10 cm giữa các điện cực kích thích và ghi. Độ trễ vận động xa trung bình dài hơn một chút, nhưng có ý nghĩa sau ES so với MS (lần lượt là 2,5 +/- 0,4 ms và 2,7 +/- 0,3 ms, p < 0,01). ES và MS dẫn đến vận tốc dẫn truyền thần kinh vận động trung bình tương tự (NCV; lần lượt là 77 +/- 10 m/giây và 79 +/- 10 m/giây; p = 0,2). Biên độ của các điện thế hoạt động vận động kép (CMAP) sau kích thích xa so với kích thích gần khác nhau đáng kể ở ES (2,5 so với 1,9 mV; p < 0,01) và MS (3,6 so với 1,6 mV; tương ứng p < 0,001). Ở một đối tượng, cả ES xa và gần đều không gây ra CMAP. Ở tám đối tượng, ES gần không tạo ra CMAP. Ở tất cả các đối tượng, MS xa dẫn đến CMAP có thể nhìn thấy được, trong khi ở hai đối tượng, MS gần không thành công. Do đó, MS đã được chứng minh là hiệu quả hơn đáng kể trong việc tạo ra CMAP (p < 0,05). Hơn nữa, MS được đồng ý là ít khó chịu hơn ES. Tóm lại, MS tạo điều kiện thuận lợi cho các nghiên cứu dẫn truyền thần kinh của IN và vượt trội hơn ES về hiệu suất và khả năng chấp nhận."} +{"idx": 1950059, "en": "This paper reviews the key issues presented during the Fourth International Conference on Schizophrenia, which was held in October 1996 in Vancouver, Canada. The main emphasis was placed on the problem of stigma, loneliness and work as well as on the necessity to further elucidate the physiopathology of schizophrenia. Some of the barriers discussed are unlikely to disappear from human societies in the short term with any possible cure for schizophrenia as they are part of any major long-term illness, of which there is a long and ever increasing list.", "gemini": "Bài báo này xem xét các vấn đề chính được trình bày trong Hội nghị quốc tế lần thứ tư về bệnh tâm thần phân liệt, được tổ chức vào tháng 10 năm 1996 tại Vancouver, Canada. Trọng tâm chính được đặt vào vấn đề kỳ thị, cô đơn và công việc cũng như sự cần thiết phải làm sáng tỏ thêm về sinh lý bệnh của bệnh tâm thần phân liệt. Một số rào cản được thảo luận không có khả năng biến mất khỏi các xã hội loài người trong thời gian ngắn với bất kỳ phương pháp chữa trị nào có thể có đối với bệnh tâm thần phân liệt vì chúng là một phần của bất kỳ bệnh lâu dài nghiêm trọng nào, trong đó có một danh sách dài và ngày càng tăng."} +{"idx": 1950060, "en": "The aim of this study was to describe mortality, mode of death and risk indicators for death during 5 years of follow-up among men and women coming to the emergency department with chest pain or other symptoms raising suspicion of acute myocardial infarction (AMI). During the 21 months of the study, all patients who came to the medical emergency department of one single hospital with chest pain or other symptoms suggestive of AMI were prospectively followed for 5 years. A total of 5362 patients came on 7157 occasions; men accounted for 55% of the admissions. The 5-year mortality rate was 25.6% for men compared with 25.7% for women. The women were older and had a higher prevalence of known congestive heart failure and hypertension, whereas the prevalence of previous myocardial infarction was higher in men. When correcting for dissimilarities in age and clinical history, male gender appeared as an independent predictor of death. In terms of mode of death men differed from wommore frequently dying at home, more frequently dying in association with ventricular fibrillation and less frequently dying in association with congestive heart failure. However, these differences were to some extent explained by differences in age. Independent risk indicators for death during 5 years of follow-up differed in men and women. It was concluded that in a consecutive series of patients with chest pain or other symptoms suggesting AMI in the emergency department, male gender was an independent risk indicator for death during a 5-year follow-up. This might be explained by a higher occurrence of coronary artery disease in men than in women in this patient population.", "gemini": "Mục đích của nghiên cứu này là mô tả tỷ lệ tử vong, phương thức tử vong và các chỉ số nguy cơ tử vong trong 5 năm theo dõi ở nam giới và phụ nữ đến khoa cấp cứu vì đau ngực hoặc các triệu chứng khác khiến nghi ngờ nhồi máu cơ tim cấp (AMI). Trong suốt 21 tháng nghiên cứu, tất cả bệnh nhân đến khoa cấp cứu y tế của một bệnh viện duy nhất vì đau ngực hoặc các triệu chứng khác gợi ý AMI đều được theo dõi trong 5 năm. Tổng cộng có 5362 bệnh nhân đến khám 7157 lần; nam giới chiếm 55% số lần nhập viện. Tỷ lệ tử vong 5 năm là 25,6% ở nam giới so với 25,7% ở nữ giới. Phụ nữ lớn tuổi hơn và có tỷ lệ mắc suy tim sung huyết và tăng huyết áp cao hơn, trong khi tỷ lệ nhồi máu cơ tim trước đó cao hơn ở nam giới. Khi hiệu chỉnh sự khác biệt về tuổi tác và tiền sử lâm sàng, giới tính nam xuất hiện như một yếu tố dự báo tử vong độc lập. Về phương thức tử vong, nam giới khác với nữ giới khi thường tử vong ở nhà, thường tử vong liên quan đến rung thất và ít tử vong liên quan đến suy tim sung huyết hơn. Tuy nhiên, những khác biệt này ở một mức độ nào đó được giải thích bởi sự khác biệt về tuổi tác. Các chỉ số nguy cơ tử vong độc lập trong 5 năm theo dõi khác nhau ở nam và nữ. Kết luận rằng trong một loạt các bệnh nhân liên tiếp bị đau ngực hoặc các triệu chứng khác gợi ý AMI tại khoa cấp cứu, giới tính nam là một chỉ số nguy cơ tử vong độc lập trong thời gian theo dõi 5 năm. Điều này có thể được giải thích bằng tỷ lệ mắc bệnh động mạch vành cao hơn ở nam giới so với nữ giới trong nhóm bệnh nhân này."} +{"idx": 1950061, "en": "Resuscitation (CPR) courses stress acquisition of psychomotor skills. The number of mannequins may limit the 'hands-on' time available for each trainee to practise CPR and impede acquisition of skill. This may occur because expensive, sophisticated mannequins are favoured over individual, simple mannequins. In a blind, prospective, controlled study we compared one-rescuer CPR skills of 165 trainees in two cohorts using their own individual light-weight torso mannequins (Actar 911 and Laerdal Little Anne) and a control cohort with four to five trainees sharing a sophisticated mannequin (Laerdal Recording Resusci Anne). No major significant differences (p = 0.18) were found when using the 'Berden scoring system'. Both the Actar 911 and the Little Anne were compatible with the Recording Resusci Anne. Trainees preferred the individual mannequins. We conclude that the results indicate that the use of individual mannequins in conjunction with a sophisticated mannequin neither results in trainees learning incorrect skills nor in significant improvement. Further analysis of the actual training in lay person CPR training courses and evaluation of course didactics to optimize training time appear indicated.", "gemini": "Các khóa học hồi sức tim phổi (CPR) nhấn mạnh việc tiếp thu các kỹ năng vận động. Số lượng ma-nơ-canh có thể hạn chế thời gian 'thực hành' mà mỗi học viên có thể thực hành CPR và cản trở việc tiếp thu kỹ năng. Điều này có thể xảy ra vì những ma-nơ-canh đắt tiền và tinh vi được ưa chuộng hơn những ma-nơ-canh đơn giản, riêng lẻ. Trong một nghiên cứu đối chứng, có kiểm soát, mù, chúng tôi đã so sánh các kỹ năng CPR của một nhân viên cứu hộ ở 165 học viên trong hai nhóm bằng cách sử dụng ma-nơ-canh thân nhẹ riêng của họ (Actar 911 và Laerdal Little Anne) và một nhóm đối chứng với bốn đến năm học viên chia sẻ một ma-nơ-canh tinh vi (Laerdal Recording Resusci Anne). Không có sự khác biệt đáng kể nào (p = 0,18) khi sử dụng 'hệ thống chấm điểm Berden'. Cả Actar 911 và Little Anne đều tương thích với Recording Resusci Anne. Các học viên thích những ma-nơ-canh riêng lẻ. Chúng tôi kết luận rằng các kết quả chỉ ra rằng việc sử dụng ma-nơ-canh riêng lẻ kết hợp với ma-nơ-canh tinh vi không dẫn đến việc học viên học các kỹ năng không chính xác cũng như không cải thiện đáng kể. Việc phân tích thêm về đào tạo thực tế trong các khóa đào tạo CPR cho người dân thường và đánh giá phương pháp giảng dạy của khóa học để tối ưu hóa thời gian đào tạo có vẻ được chỉ định."} +{"idx": 1950062, "en": "The objective of this study was to determine the methods of anaesthesia used for the reduction of distal radius fractures in adults in Scotland and to compare this with the UK situation. The method used was a telephone questionnaire of accident and emergency doctors in 25 Scottish hospitals dealing with trauma. Thirty-two per cent still use general anaesthesia routinely for these procedures despite its cost, complexity and need for admission in the majority of cases. Forty-four per cent now use the Bier's block technique of regional anaesthesia, with the attendant advantages of ease of use, rapid recovery and no need for admission in the majority of patients. Twelve per cent use intravenous sedation and 12% use haematoma blocks for manipulations. It is concluded that Bier's block may be the anaesthetic method of choice for the management of distal radius fractures both in efficiency and economic terms in Scotland. However, training for Bier's blocks needs to be standardized and improved.", "gemini": "Mục tiêu của nghiên cứu này là xác định các phương pháp gây mê được sử dụng để nắn gãy xương quay xa ở người lớn tại Scotland và so sánh phương pháp này với tình hình ở Vương quốc Anh. Phương pháp được sử dụng là bảng câu hỏi qua điện thoại với các bác sĩ cấp cứu tại 25 bệnh viện ở Scotland chuyên điều trị chấn thương. Ba mươi hai phần trăm vẫn sử dụng gây mê toàn thân thường quy cho các thủ thuật này mặc dù chi phí, phức tạp và cần phải nhập viện trong phần lớn các trường hợp. Bốn mươi bốn phần trăm hiện sử dụng kỹ thuật gây tê vùng Bier's block, với các lợi thế kèm theo là dễ sử dụng, phục hồi nhanh và không cần nhập viện ở phần lớn bệnh nhân. Mười hai phần trăm sử dụng thuốc an thần tĩnh mạch và 12% sử dụng khối tụ máu để thao tác. Kết luận rằng khối Bier có thể là phương pháp gây mê được lựa chọn để xử trí gãy xương quay xa cả về hiệu quả và kinh tế ở Scotland. Tuy nhiên, việc đào tạo về khối Bier cần được chuẩn hóa và cải thiện."} +{"idx": 1950063, "en": "It is well recognized by accident and emergency clinicians and the defence societies that radiological abnormalities are frequently missed by the clinician who first looks at the X-rays. These errors may be compounded if the X-rays are not reported by a radiologist. The X-rays may be reported but the report is not seen by the clinician. The report may be seen by the clinician, but a system for recalling the patient is not put into operation. The purpose of this article is to make the important point that false positive and false negative misses on X-rays need to be communicated to the accident and emergency department promptly so that appropriate action can be taken. We describe an efficient method of communication.", "gemini": "Các bác sĩ lâm sàng về tai nạn và cấp cứu cũng như các hội bảo vệ đều công nhận rằng các bác sĩ lâm sàng thường bỏ sót các bất thường về X quang khi lần đầu xem phim X quang. Những lỗi này có thể trở nên nghiêm trọng hơn nếu phim X quang không được bác sĩ chuyên khoa chẩn đoán hình ảnh báo cáo. Phim X quang có thể được báo cáo nhưng bác sĩ lâm sàng không xem báo cáo. Bác sĩ lâm sàng có thể đã xem báo cáo nhưng không đưa vào hoạt động hệ thống triệu hồi bệnh nhân. Mục đích của bài viết này là nêu ra điểm quan trọng rằng những trường hợp bỏ sót dương tính giả và âm tính giả trên phim X quang cần được thông báo kịp thời cho khoa tai nạn và cấp cứu để có thể thực hiện hành động thích hợp. Chúng tôi mô tả một phương pháp truyền đạt hiệu quả."} +{"idx": 1950064, "en": "Mass gatherings are special situations for which mass medical care must be preplanned. Acute emergencies occur at public gatherings and medical coverage on site has proven benefit. Responsibility of general plan, management of specific problems, transport planning, communications system, guidelines and protocols, special situations management, ancillary supports, sources of extra help for unforeseen needs are the most important items to consider. In mass gatherings the whole emergency medical service (EMS) planning and management has to depend on the emergency department direction, with its authority on all aspects of patient care in the EMS system. This report concerns the planning of EMS and of medical care in a situation at risk for mass casualties at the Formula I Grand Prix-Championship Racing 'San Marino' of Imola.", "gemini": "Các cuộc tụ tập đông người là những tình huống đặc biệt mà việc chăm sóc y tế đại chúng phải được lập kế hoạch trước. Các trường hợp cấp cứu xảy ra tại các cuộc tụ tập đông người và việc bảo vệ y tế tại chỗ đã được chứng minh là có lợi. Trách nhiệm của kế hoạch chung, quản lý các vấn đề cụ thể, lập kế hoạch vận chuyển, hệ thống thông tin liên lạc, hướng dẫn và giao thức, quản lý các tình huống đặc biệt, hỗ trợ phụ trợ, nguồn trợ giúp thêm cho các nhu cầu không lường trước là những vấn đề quan trọng nhất cần xem xét. Trong các cuộc tụ tập đông người, toàn bộ kế hoạch và quản lý dịch vụ y tế khẩn cấp (EMS) phải phụ thuộc vào sự chỉ đạo của khoa cấp cứu, với thẩm quyền của khoa này đối với mọi khía cạnh chăm sóc bệnh nhân trong hệ thống EMS. Báo cáo này liên quan đến việc lập kế hoạch EMS và chăm sóc y tế trong tình huống có nguy cơ xảy ra thương vong hàng loạt tại Giải đua xe Công thức 1 Grand Prix-Championship 'San Marino' của Imola."} +{"idx": 1950065, "en": "We have evaluated the level of satisfaction of patients assigned to a social worker whose role was to give information, support, advice, grief and shock counselling; and develop resource strategies for marginal peoples. Six hundred patients were admitted to the study; 300 of them were supervised by a social worker (group A) and the other 300 were not (group B). After the completion of the study, a questionnaire was sent to be filled in by group A and group B for later evaluation. We collected 200 completed questionnaires (33.3%). The most important areas were: 105 patients (94.5%) in group A vs. 73 patients (82%) (p < 0.005) consider the medical care good or very good. The waiting time was assessed as short or acceptable in 91 patients (81.9%) in group A vs. 55 patients (61.8%) in group B (p < 0.001). The psychosocial care was assessed as very good or good in 101 patients group A (90.9%) vs. 73 patients in group B (82%) (p < 0.5).", "gemini": "Chúng tôi đã đánh giá mức độ hài lòng của những bệnh nhân được chỉ định cho một nhân viên xã hội có vai trò cung cấp thông tin, hỗ trợ, tư vấn, tư vấn về đau buồn và sốc; và phát triển các chiến lược về nguồn lực cho những người dân thiểu số. Sáu trăm bệnh nhân đã được đưa vào nghiên cứu; 300 trong số họ được một nhân viên xã hội giám sát (nhóm A) và 300 người còn lại thì không (nhóm B). Sau khi hoàn thành nghiên cứu, một bảng câu hỏi đã được gửi đến để nhóm A và nhóm B điền vào để đánh giá sau. Chúng tôi đã thu thập được 200 bảng câu hỏi đã hoàn thành (33,3%). Các lĩnh vực quan trọng nhất là: 105 bệnh nhân (94,5%) ở nhóm A so với 73 bệnh nhân (82%) (p < 0,005) cho rằng dịch vụ chăm sóc y tế là tốt hoặc rất tốt. Thời gian chờ được đánh giá là ngắn hoặc chấp nhận được ở 91 bệnh nhân (81,9%) ở nhóm A so với 55 bệnh nhân (61,8%) ở nhóm B (p < 0,001). Việc chăm sóc tâm lý xã hội được đánh giá là rất tốt hoặc tốt ở 101 bệnh nhân nhóm A (90,9%) so với 73 bệnh nhân ở nhóm B (82%) (p < 0,5)."} +{"idx": 1950066, "en": "Previous studies have indicated that respiratory sound signals may contain information useful in the detection of lung diseases. In this study, measurement and recordings of respiratory sound signal segments were obtained in normal subjects (non-smokers) and smokers in both inspiration and expiration phases. By using the autoregressive (AR) method, it is possible to produce power spectra of respiratory sound signals in inspiration and expiration phases for smokers and non-smokers of each group. The selection of the AR model order of the respiratory sound signals is achieved using Akaike criterion. The AR model order of 9 is required for completely described respiration sound signal segments in inspiration and expiration phases for both groups. The power spectra in the smoker group show larger distinct peaks at lower frequencies as well as more harmonics in both inspiration and expiration phases compared to the power spectra of the non-smoker group. Another diagnostic indicator was derived from the relative position of poles of the AR model of respiratory sound signals. In all smokers it was found that the first, third and fourth poles were closer to a unit circle than those in non-smokers (P < 0.01). It seems that the use of these indicators may be useful as early diagnostic tool for lung diseases.", "gemini": "Các nghiên cứu trước đây đã chỉ ra rằng các tín hiệu âm thanh hô hấp có thể chứa thông tin hữu ích trong việc phát hiện các bệnh về phổi. Trong nghiên cứu này, các phép đo và bản ghi của các đoạn tín hiệu âm thanh hô hấp đã thu được ở những đối tượng bình thường (không hút thuốc) và những người hút thuốc trong cả giai đoạn hít vào và thở ra. Bằng cách sử dụng phương pháp tự hồi quy (AR), có thể tạo ra phổ công suất của các tín hiệu âm thanh hô hấp trong giai đoạn hít vào và thở ra đối với người hút thuốc và không hút thuốc của mỗi nhóm. Việc lựa chọn bậc mô hình AR của các tín hiệu âm thanh hô hấp đạt được bằng cách sử dụng tiêu chuẩn Akaike. Bậc mô hình AR là 9 là cần thiết để mô tả hoàn toàn các đoạn tín hiệu âm thanh hô hấp trong giai đoạn hít vào và thở ra đối với cả hai nhóm. Phổ công suất ở nhóm người hút thuốc cho thấy các đỉnh riêng biệt lớn hơn ở tần số thấp cũng như nhiều sóng hài hơn ở cả giai đoạn hít vào và thở ra so với phổ công suất của nhóm không hút thuốc. Một chỉ số chẩn đoán khác có được từ vị trí tương đối của các cực của mô hình AR của các tín hiệu âm thanh hô hấp. Ở tất cả những người hút thuốc, người ta thấy rằng cực thứ nhất, thứ ba và thứ tư gần với một vòng tròn đơn vị hơn so với những người không hút thuốc (P < 0,01). Có vẻ như việc sử dụng các chỉ số này có thể hữu ích như một công cụ chẩn đoán sớm cho các bệnh về phổi."} +{"idx": 1950067, "en": "With a view to normalization of the head orientation without the use of a fixation jig for the measurement of jaw movement employing an X-ray television, we have been developing an automatic head-positioning system, Racs-N, consisting of a head-position sensor and a robotized chair. The head position is monitored in 6 degrees of freedom (d.o.f.) by using a position-sensitive detector (PSD) camera-based photostereometry. If they are incorrect, the robotized chair rectifies them automatically by adjusting the position and orientation of the head support in 5 d.o.f. (except the vertical position). The rectified position, however, has a certain margin of error, due to the mobility of the head relative to the head support. To improve this, we employed an iterative correction in that the positioning is repeated until the determined position converges. In experiments employing several subjects, this particular correction was verified to converge by more than six iterations and to be effective for the reduction of head-position errors.", "gemini": "Với mục đích chuẩn hóa hướng đầu mà không cần sử dụng giá cố định để đo chuyển động hàm bằng cách sử dụng truyền hình X quang, chúng tôi đã phát triển một hệ thống định vị đầu tự động, Racs-N, bao gồm cảm biến vị trí đầu và ghế rô bốt. Vị trí đầu được theo dõi ở 6 bậc tự do (d.o.f.) bằng cách sử dụng phép đo ảnh lập thể dựa trên camera phát hiện vị trí (PSD). Nếu chúng không chính xác, ghế rô bốt sẽ tự động chỉnh sửa chúng bằng cách điều chỉnh vị trí và hướng của giá đỡ đầu ở 5 d.o.f. (trừ vị trí thẳng đứng). Tuy nhiên, vị trí được chỉnh sửa có một biên độ lỗi nhất định, do tính di động của đầu so với giá đỡ đầu. Để cải thiện điều này, chúng tôi đã sử dụng một phép hiệu chỉnh lặp lại ở chỗ việc định vị được lặp lại cho đến khi vị trí xác định hội tụ. Trong các thí nghiệm sử dụng nhiều đối tượng, phép hiệu chỉnh cụ thể này đã được xác minh là hội tụ sau hơn sáu lần lặp và có hiệu quả trong việc giảm lỗi vị trí đầu."} +{"idx": 1950068, "en": "Considering the management and safety of the insulin delivery route when a wearable artificial endocrine pancreas is applied to ambulatory diabetic patients on a long-term basis, we developed a s.c. insulin infusion algorithm by analyzing the dynamics of a s.c. injected short-acting insulin analog (Insulin Lispro) by a three-compartment model. Principally the insulin infusion algorithm was developed as a transfer function with the first-order delay in both proportional and derivative actions to blood glucose concentrations. The parameters for this algorithm were calculated to simulate a physiological plasma insulin profile as closely as possible. By applying this algorithm with regular insulin, diabetic patients showed a 2 h postprandial hyperglycemia and a delayed hyperinsulinemia, followed by hypoglycemic episodes 4-5 h after oral glucose load, just as observed in the computer simulation study. However, using Insulin Lispro, a near-physiological glycemic control (postprandial blood glucose of 153.1 +/- 8.3 mg/100 ml at 60 min and 90.3 +/- 7.1 mg/100 ml at 180 min, respectively) could be achieved without showing any delayed hyperinsulinemia or hypoglycemia. Daily glycemic excursions were also controlled near-physiologically and although the daily insulin requirement (731.7 +/- 160.5 mU/kg/day) was slightly higher, it was not significantly different from that with i.v. insulin infusion (622.3 +/- 142.6 mU/kg/day). These results indicate that the application of s.c. insulin infusion algorithm with Insulin Lispro is feasible for long-term glycemic control with a wearable artificial endocrine pancreas in ambulatory diabetic patients.", "gemini": "Xem xét việc quản lý và tính an toàn của đường truyền cung cấp insulin khi tuyến tụy nội tiết nhân tạo đeo được áp dụng cho bệnh nhân tiểu đường ngoại trú trong thời gian dài, chúng tôi đã phát triển một thuật toán truyền insulin s.c. bằng cách phân tích động lực học của chất tương tự insulin tác dụng ngắn tiêm s.c. (Insulin Lispro) theo mô hình ba ngăn. Về nguyên tắc, thuật toán truyền insulin được phát triển như một hàm truyền với độ trễ bậc nhất trong cả các tác động tỷ lệ và đạo hàm đối với nồng độ glucose trong máu. Các thông số cho thuật toán này được tính toán để mô phỏng hồ sơ insulin huyết tương sinh lý càng gần càng tốt. Bằng cách áp dụng thuật toán này với insulin thường, bệnh nhân tiểu đường biểu hiện tăng đường huyết sau ăn 2 giờ và tăng insulin huyết chậm, tiếp theo là các đợt hạ đường huyết 4-5 giờ sau khi uống glucose, giống như quan sát thấy trong nghiên cứu mô phỏng máy tính. Tuy nhiên, khi sử dụng Insulin Lispro, có thể đạt được kiểm soát đường huyết gần như sinh lý (đường huyết sau ăn là 153,1 +/- 8,3 mg/100 ml tại phút thứ 60 và 90,3 +/- 7,1 mg/100 ml tại phút thứ 180) mà không biểu hiện tăng insulin huyết chậm hoặc hạ đường huyết. Biến động đường huyết hàng ngày cũng được kiểm soát gần như sinh lý và mặc dù nhu cầu insulin hàng ngày (731,7 +/- 160,5 mU/kg/ngày) cao hơn một chút, nhưng không khác biệt đáng kể so với truyền insulin tĩnh mạch (622,3 +/- 142,6 mU/kg/ngày). Những kết quả này chỉ ra rằng việc áp dụng thuật toán truyền insulin s.c. với Insulin Lispro là khả thi để kiểm soát đường huyết dài hạn với tuyến tụy nội tiết nhân tạo đeo được ở bệnh nhân tiểu đường ngoại trú."} +{"idx": 1950069, "en": "Recently high quality ECG records have been obtained by means of microdot thermal printers, because of their numerous advantages. Usually they contain a shift register in the thermal head and the fast control of the dot locations to be heated is an important software problem. We implemented the TLP 480Z-N Gotemba thermal printer (made in Japan) in our ECG instruments, and elaborated two algorithms for lengthwise and crosswise printing on the paper. They use 'black and white' strips loaded into the shift register by 1-byte parallel data organization. The lengthwise mode can operate in real time, while the crosswise one is intended for memorized signals.", "gemini": "Gần đây, các bản ghi ECG chất lượng cao đã được thu được bằng máy in nhiệt vi điểm, vì chúng có nhiều ưu điểm. Thông thường, chúng chứa một thanh ghi dịch trong đầu nhiệt và việc kiểm soát nhanh vị trí các điểm cần làm nóng là một vấn đề phần mềm quan trọng. Chúng tôi đã triển khai máy in nhiệt TLP 480Z-N Gotemba (sản xuất tại Nhật Bản) trong các dụng cụ ECG của mình và xây dựng hai thuật toán để in theo chiều dọc và chiều ngang trên giấy. Chúng sử dụng các dải 'đen và trắng' được tải vào thanh ghi dịch theo tổ chức dữ liệu song song 1 byte. Chế độ theo chiều dọc có thể hoạt động theo thời gian thực, trong khi chế độ theo chiều ngang dành cho các tín hiệu được ghi nhớ."} +{"idx": 1950070, "en": "The purpose of the present investigation was to develop a mattress which was made of the new synthetic fibers called 'Shin-Gosen', and to determine its physical characteristics associated with pressure sores such as pressure distribution, temperature and humidity. The Shin-Gosen mattress consisted of three layers of elastic fibers made of polyester multifilaments, which were mediated by four layers of wave-like fabrics made of nylon monofilaments. The physical characteristics of the mattress were compared with (a) the conventional cotton hospital mattress and (b) the SORELESS MAT made of vacuole gel which effectively eliminated compression forces. The Shin-Gosen mattress was found to provide pressure relief effects similar to that of the SORELESS MAT the desired thermal insulation as well as that of the cotton mattress and a higher level of moisture vapor permeability. These excellent features will not only contribute to preventing pressure sores, but will also enable comfortable resting and sleeping.", "gemini": "Mục đích của cuộc điều tra hiện tại là phát triển một tấm nệm được làm bằng loại sợi tổng hợp mới có tên gọi là 'Shin-Gosen' và xác định các đặc điểm vật lý của nó liên quan đến các vết loét do tì đè như phân bố áp suất, nhiệt độ và độ ẩm. Tấm nệm Shin-Gosen bao gồm ba lớp sợi đàn hồi làm bằng sợi đa sợi polyester, được trung gian bởi bốn lớp vải dạng sóng làm bằng sợi đơn sợi nylon. Các đặc tính vật lý của tấm nệm đã được so sánh với (a) tấm nệm bệnh viện bằng bông thông thường và (b) TẤM SORELESS làm bằng gel chân không giúp loại bỏ hiệu quả các lực nén. Tấm nệm Shin-Gosen được phát hiện có tác dụng giảm áp lực tương tự như TẤM SORELESS, khả năng cách nhiệt mong muốn cũng như tấm nệm bông và khả năng thấm hơi ẩm cao hơn. Những tính năng tuyệt vời này không chỉ góp phần ngăn ngừa loét do tì đè mà còn giúp nghỉ ngơi và ngủ thoải mái."} +{"idx": 1950071, "en": "In this paper we present a brief overview of the growing concern to standardize definitions and terminology in meta-analysis. This tool has become inescapable in both drug research and therapeutic evaluation. The performed and published meta-analyses are increasing, as well as the variation in the meaning of the terms used in meta-analysis. In the second part of the paper we propose glossary of the most common terms used in reports of meta-analyses. The glossary has been written by only one group of scientists, the definitions are therefore proposed to the scientific community as working definitions, to be subject to reactions from leaders in meta-analysis.", "gemini": "Trong bài báo này, chúng tôi trình bày một bản tóm tắt ngắn gọn về mối quan tâm ngày càng tăng đối với các định nghĩa và thuật ngữ chuẩn trong phân tích tổng hợp. Công cụ này đã trở nên không thể thiếu trong cả nghiên cứu thuốc và đánh giá điều trị. Các phân tích tổng hợp được thực hiện và công bố ngày càng tăng, cũng như sự khác biệt về ý nghĩa của các thuật ngữ được sử dụng trong phân tích tổng hợp. Trong phần thứ hai của bài báo, chúng tôi đề xuất một bảng thuật ngữ của các thuật ngữ thông dụng nhất được sử dụng trong các báo cáo phân tích tổng hợp. Bảng thuật ngữ chỉ được viết bởi một nhóm các nhà khoa học, do đó các định nghĩa được đề xuất cho cộng đồng khoa học như định nghĩa làm việc, để chịu sự phản ứng từ các nhà lãnh đạo trong phân tích tổng hợp."} +{"idx": 1950072, "en": "The effects of NKH477, a water-soluble forskolin derivative, and dibutyryl-cyclic adenosine monophosphate (dbcAMP) on the release of adrenal catecholamines (CAs) in response to splanchnic nerve stimulation (SNS), acetylcholine (ACh), the nicotinic receptor stimulant 1,1-dimethyl-4-phenyl-piperazinium iodide (DMPP) and muscarine were examined in anesthetized dogs. NKH477, dbcAMP and the cholinergic agonists were infused and injected, respectively, into the adrenal gland intra-arterially. SNS (3 Hz) or injections of ACh (3 micrograms), DMPP (2 micrograms) and muscarine (2 micrograms) produced increases in CA output determined from adrenal venous blood. Both NKH477 infusion (0.3, 1 and 3 micrograms/min) and dbcAMP infusion (0.1, 0.3 and 1 mg/min) caused dose-dependent enhancement of the SNS-, ACh- and DMPP-induced increases in CA output, whereas they failed to affect the muscarine-induced increases in CA output. Neither NKH477 nor dbcAMP affected basal CA output. Cyclic AMP (cAMP) overflow determined from adrenal venous blood increased during NKH477 infusion. These results indicate that NKH477 and dbcAMP have facilitatory effects on adrenal CA release mediated by nicotinic receptors, but not by muscarinic receptors in the dog, and suggest the selective action of cAMP on nicotinic mechanism.", "gemini": "Tác dụng của NKH477, một dẫn xuất forskolin tan trong nước và dibutyryl-cyclic adenosine monophosphate (dbcAMP) đối với sự giải phóng catecholamin tuyến thượng thận (CA) đáp ứng với kích thích thần kinh tạng (SNS), acetylcholine (ACh), chất kích thích thụ thể nicotinic 1,1-dimethyl-4-phenyl-piperazinium iodide (DMPP) và muscarine đã được kiểm tra ở những con chó được gây mê. NKH477, dbcAMP và các chất chủ vận cholinergic lần lượt được truyền và tiêm vào động mạch tuyến thượng thận. SNS (3 Hz) hoặc tiêm ACh (3 microgam), DMPP (2 microgam) và muscarine (2 microgam) làm tăng lượng CA đầu ra được xác định từ máu tĩnh mạch tuyến thượng thận. Cả dịch truyền NKH477 (0,3, 1 và 3 microgam/phút) và dịch truyền dbcAMP (0,1, 0,3 và 1 mg/phút) đều gây ra sự tăng cường phụ thuộc vào liều đối với sự gia tăng CA do SNS, ACh và DMPP gây ra, trong khi chúng không ảnh hưởng đến sự gia tăng CA do muscarine gây ra. Cả NKH477 và dbcAMP đều không ảnh hưởng đến lượng CA cơ bản. Lượng cAMP tuần hoàn được xác định từ máu tĩnh mạch tuyến thượng thận tăng lên trong quá trình truyền NKH477. Những kết quả này chỉ ra rằng NKH477 và dbcAMP có tác dụng tạo thuận lợi cho việc giải phóng CA tuyến thượng thận thông qua thụ thể nicotinic, nhưng không phải thông qua thụ thể muscarinic ở chó và gợi ý về tác dụng chọn lọc của cAMP đối với cơ chế nicotinic."} +{"idx": 1950073, "en": "Sodium nitroprusside (SNP) significantly decreased basal dopamine (DA) release when rat striatal slices were incubated in a physiological medium deficient in antioxidants. Depolarization-induced DA release (KCl 25 mM), which was accompanied with a 85% decline in tissue DA levels, was also inhibited by SNP and hydroxylamine (HA). Contrary to these findings, SNP did not protect the slices against depolarization-induced DA depletion. With HA, moreover, tissue DA levels were found to be depleted more than the control levels, indicating that DA, which is released from or stored in the slices, might be converted to an undetectable product under these conditions. Supporting this conclusion, incubation of the DA standards with SNP caused a dose-dependent loss in DA levels, an effect that was reversed partially by oxyhemoglobin and inhibited completely by antioxidants. Consistently, both SNP and HA, but not L-arginine, significantly increased basal DA release when striatal slices were incubated in antioxidants-containing medium. These results indicate that nitric oxide (NO), which is generated from SNP and HA by different mechanisms, stimulates DA release from rat striatal slices. Observation of this effect, however, requires the presence of antioxidants in the medium.", "gemini": "Sodium nitroprusside (SNP) làm giảm đáng kể giải phóng dopamine (DA) cơ bản khi các lát cắt vân não của chuột được ủ trong môi trường sinh lý thiếu chất chống oxy hóa. Sự giải phóng DA do khử cực (KCl 25 mM), đi kèm với sự sụt giảm 85% nồng độ DA trong mô, cũng bị SNP và hydroxylamine (HA) ức chế. Trái ngược với những phát hiện này, SNP không bảo vệ các lát cắt khỏi sự suy giảm DA do khử cực. Hơn nữa, với HA, nồng độ DA trong mô được phát hiện là giảm nhiều hơn so với mức kiểm soát, chỉ ra rằng DA được giải phóng hoặc lưu trữ trong các lát cắt có thể được chuyển đổi thành một sản phẩm không thể phát hiện được trong những điều kiện này. Ủng hộ kết luận này, việc ủ các tiêu chuẩn DA với SNP gây ra mất DA phụ thuộc vào liều, một tác dụng bị oxyhemoglobin đảo ngược một phần và bị chất chống oxy hóa ức chế hoàn toàn. Tương tự, cả SNP và HA, nhưng không phải L-arginine, đều làm tăng đáng kể giải phóng DA cơ bản khi các lát cắt vân não được ủ trong môi trường có chứa chất chống oxy hóa. Những kết quả này chỉ ra rằng nitric oxide (NO), được tạo ra từ SNP và HA thông qua các cơ chế khác nhau, kích thích giải phóng DA từ các lát cắt vân não của chuột. Tuy nhiên, để quan sát được hiệu ứng này, cần có chất chống oxy hóa trong môi trường."} +{"idx": 1950074, "en": "Incubation of striatal slices with sodium nitroprusside (SNP) or hydroxylamine (HA) for 60 min caused a dose-dependent increase in dopamine (DA) release. This effect was inhibited completely by tetrodotoxin (TTX) (1 microM) if low concentrations of SNP (1 microM) or HA (10 and 100 microM) were tested. Although-higher concentration of SNP (10 and 100 microM) and HA (500 microM) were still effective in stimulating DA release, increases observed under these conditions were less than the values found in the absence of TTX. Verapamil (10 microM), but not omega-conotoxin (100 nM), significantly reduced DA release stimulated by high concentrations of SNP or HA. When verapamil was combined with TTX, moreover, SNP and HA failed to stimulate DA release. If striatal slices were incubated in the presence of nomifensine (10 microM), SNP and HA did not enhance DA release. SNP and HA-induced depletions in tissue DA levels were also protected by nomifensine. Inhibition of guanylate cyclase with 10 microM of methylene blue could not reduce the effects of NO-donors. SNP and HA also failed to alter endogenous glutamate release from striatal slices. Similarly, SNP and HA-induced increases in DA release were not affected by kynurenic acid and MK-801. These results indicate that NO-donors SNP and HA stimulate DA release by facilitating reverse DA transport. This effect seems to be dependent on the activation of both voltage dependent sodium channels and L-type of calcium channels. Results presented here also indicate that neither endogenous glutamate nor guanylate cyclase activation plays an intermediary role in stimulatory effects of NO-donors on DA release from rat striatal slices.", "gemini": "Việc ủ các lát cắt vân não với sodium nitroprusside (SNP) hoặc hydroxylamine (HA) trong 60 phút gây ra sự gia tăng giải phóng dopamine (DA) phụ thuộc vào liều. Hiệu ứng này bị tetrodotoxin (TTX) (1 microM) ức chế hoàn toàn nếu thử nghiệm ở nồng độ thấp của SNP (1 microM) hoặc HA (10 và 100 microM). Mặc dù nồng độ cao hơn của SNP (10 và 100 microM) và HA (500 microM) vẫn có hiệu quả trong việc kích thích giải phóng DA, nhưng sự gia tăng được quan sát thấy trong những điều kiện này thấp hơn các giá trị tìm thấy khi không có TTX. Verapamil (10 microM), nhưng không phải omega-conotoxin (100 nM), làm giảm đáng kể giải phóng DA được kích thích bởi nồng độ cao của SNP hoặc HA. Hơn nữa, khi verapamil được kết hợp với TTX, SNP và HA không kích thích giải phóng DA. Nếu các lát cắt vân não được ủ trong sự hiện diện của nomifensine (10 microM), SNP và HA không làm tăng giải phóng DA. Sự suy giảm do SNP và HA gây ra trong nồng độ DA của mô cũng được nomifensine bảo vệ. Ức chế guanylate cyclase với 10 microM xanh methylen không thể làm giảm tác dụng của các chất cho NO. SNP và HA cũng không làm thay đổi giải phóng glutamate nội sinh từ các lát cắt vân não. Tương tự, sự gia tăng giải phóng DA do SNP và HA gây ra không bị ảnh hưởng bởi axit kynurenic và MK-801. Những kết quả này chỉ ra rằng các chất cho NO là SNP và HA kích thích giải phóng DA bằng cách tạo thuận lợi cho quá trình vận chuyển DA ngược. Hiệu ứng này dường như phụ thuộc vào sự kích hoạt của cả kênh natri phụ thuộc vào điện áp và kênh canxi loại L. Các kết quả được trình bày ở đây cũng chỉ ra rằng cả glutamate nội sinh và hoạt hóa guanylate cyclase đều không đóng vai trò trung gian trong các tác dụng kích thích của các chất cho NO đối với giải phóng DA từ các lát cắt vân não của chuột."} +{"idx": 1950075, "en": "Genetically hypertensive rats (LH) of the Lyon strain, compared to their normo-tensive (LN) controls associate, in a unique manner, high blood pressure with increases in body weight and in plasma lipids and insulin/glucose ratio. The present work investigated the development of insulin resistance with age in this model. At the age of 22 and 52 weeks, LH and LN fasted male rats were submitted to an intravenous glucose tolerance test, allowing measurement of the elimination rate of the glucose and the area under the curve of the insulin response. Insulin sensitivity was calculated as the ratio of these two parameters. It was observed that insulin sensitivity coefficient decreased with age in all the animals and that LH rats did not significantly differ from LN controls (from 62.6 +/- 3.3 and 69.1 +/- 4 at 22 weeks to 42.1 +/- 4.4 and 49.5 +/- 12.8 at 52 weeks for LH and LN rats, respectively). It is concluded that 1) elevated plasma insulin/glucose ratio does not mean insulin resistance and 2) hypertension can develop without being associated, even in aged rats, to a true insulin resistance.", "gemini": "So với chuột đối chứng bình thường (LN), chuột tăng huyết áp di truyền (LH) thuộc dòng Lyon có liên quan theo một cách độc đáo, huyết áp cao với sự gia tăng trọng lượng cơ thể và lipid huyết tương và tỷ lệ insulin/glucose. Nghiên cứu hiện tại đã điều tra sự phát triển của tình trạng kháng insulin theo tuổi trong mô hình này. Ở độ tuổi 22 và 52 tuần, chuột đực LH và LN nhịn ăn đã được thử nghiệm dung nạp glucose tĩnh mạch, cho phép đo tốc độ đào thải glucose và diện tích dưới đường cong đáp ứng insulin. Độ nhạy insulin được tính bằng tỷ lệ của hai thông số này. Người ta quan sát thấy rằng hệ số độ nhạy insulin giảm theo tuổi ở tất cả các loài động vật và chuột LH không khác biệt đáng kể so với chuột đối chứng LN (từ 62,6 +/- 3,3 và 69,1 +/- 4 ở tuần thứ 22 đến 42,1 +/- 4,4 và 49,5 +/- 12,8 ở tuần thứ 52 đối với chuột LH và LN, tương ứng). Kết luận rằng 1) tỷ lệ insulin/glucose huyết tương tăng không có nghĩa là kháng insulin và 2) tăng huyết áp có thể phát triển mà không liên quan, ngay cả ở chuột già, đến tình trạng kháng insulin thực sự."} +{"idx": 1950076, "en": "Adenosine tri- and diphosphate (ATP and ADP) and their structural analogues stimulate insulin secretion from the isolated perfused rat pancreas, an effect mediated by P2Y-purinoceptor activation. Concerning the base moiety of the nucleotide, it was previously shown that purine but not pyrimidine nucleoside triphosphates were active and that substitution on purine C2 with the 2-methylthio group greatly enhanced the potency. In this study, we further analyze the consequences of ribose and polyphosphate chain modifications. Modifications in 2' and 3' position on the ribose led to a decrease in insulin response when bulky substitutions were made: indeed, 2'-deoxy ATP was similar in activity to ATP, whereas arylazido-aminopropionyl ATP (ANAPP3) was weakly effective and trinitrophenyl ATP (TNP-ATP) was inactive. Substitution on the gamma phosphorus of the triphosphate chain led to a decrease (gamma-anilide ATP) or no change (gamma-azido ATP) in potency; the replacement of the bridging oxygen between beta and gamma phosphorus by a peroxide group did not significantly change the activity, whereas substitution by a methylene group completely abolished stimulation of insulin secretion. As for the phosphorothioate analogues, adenosine-5'-O-(3-thiotriphosphate) (ATP gamma S) induced an insulin response similar to that produced by ATP, whereas adenosine-5'-O-(2-thiodiphosphate) (ADP beta S) was about 100-fold more potent than ATP, as previously shown. In conclusion, two structural features seem to have a strategic importance for increasing the insulin secretory activity of ATP analogues: substitution at the C2 position on the adenine ring of ATP and modifications of the polyphosphate chain at the level of the beta phosphorus.", "gemini": "Adenosine tri- và diphosphate (ATP và ADP) và các chất tương tự về mặt cấu trúc của chúng kích thích tiết insulin từ tuyến tụy của chuột được tưới máu riêng biệt, một tác dụng được trung gian bởi hoạt hóa thụ thể P2Y-purinoceptor. Về phần gốc bazơ của nucleotide, trước đây đã được chứng minh rằng các nucleoside triphosphate purine nhưng không phải pyrimidine có hoạt tính và sự thay thế ở purine C2 bằng nhóm 2-methylthio làm tăng đáng kể hiệu lực. Trong nghiên cứu này, chúng tôi phân tích thêm các hậu quả của các sửa đổi chuỗi ribose và polyphosphate. Các sửa đổi ở vị trí 2' và 3' trên ribose dẫn đến giảm đáp ứng insulin khi thực hiện các thay thế cồng kềnh: thực sự, ATP 2'-deoxy có hoạt tính tương tự như ATP, trong khi arylazido-aminopropionyl ATP (ANAPP3) có hiệu quả yếu và trinitrophenyl ATP (TNP-ATP) không hoạt động. Sự thay thế trên gamma phốt pho của chuỗi triphosphate dẫn đến giảm (gamma-anilide ATP) hoặc không thay đổi (gamma-azido ATP) về hiệu lực; việc thay thế oxy cầu nối giữa beta và gamma phốt pho bằng nhóm peroxide không làm thay đổi đáng kể hoạt động, trong khi thay thế bằng nhóm methylene thì hoàn toàn không còn kích thích tiết insulin. Đối với các chất tương tự phosphorothioate, adenosine-5'-O-(3-thiotriphosphate) (ATP gamma S) gây ra đáp ứng insulin tương tự như đáp ứng do ATP tạo ra, trong khi adenosine-5'-O-(2-thiodiphosphate) (ADP beta S) mạnh hơn ATP khoảng 100 lần, như đã trình bày trước đây. Tóm lại, hai đặc điểm cấu trúc dường như có tầm quan trọng chiến lược để tăng hoạt tính tiết insulin của các chất tương tự ATP: sự thay thế ở vị trí C2 trên vòng adenine của ATP và các sửa đổi chuỗi polyphosphate ở mức phốt pho beta."} +{"idx": 1950077, "en": "Yohimbine has been proposed for the treatment of neurogenic orthostatic hypotension; however, no controlled trial has been performed in experimental models of orthostatic hypotension or in patients with autonomic failure. The aim of the present study was to compare the effects of yohimbine (0.05 mg/kg, intravenously [i.v.]) and placebo (saline) in a new model of neurogenic orthostatic hypotension obtained by sinoaortic denervation (SAD) in chloralose-anaesthetized dogs. Blood pressure, heart rate, noradrenaline plasma levels and systolic blood pressure and heart rate short-term variabilities (calculated on low frequency [40-50 MHz] and high frequency [390-490 MHz] bands) were measured in supine position and after a 10 min 80 degrees head-up tilting. The drugs were administered in a double-blind cross-over randomized fashion. The head-up tilting performed in normal animals increased diastolic blood pressure (+12 +/- 4 mmHg), heart rate (+39 +/- 12 beats per minute [bpm]), the low frequency band of systolic blood pressure and noradrenaline plasma level, without changing systolic blood pressure or heart rate variability. In SAD dogs, a marked fall in systolic (-80 +/- 11 mmHg) and diastolic (-43 +/- 4 mmHg) blood pressures was observed within 1 min after placebo, without modification in heart rate, systolic blood pressure and heart rate short-term variabilities and noradrenaline plasma levels. In SAD dogs, yohimbine (0.05 mg/kg, i.v.) delayed the blood pressure fall elicited by head-up tilting, but failed to modify its magnitude. These results show that, in the model of orthostatic hypotension obtained by SAD, yohimbine, at an alpha 2-adrenoceptor selective dose (0.05 mg/kg), delays the fall in blood pressure elicited by head-up tilting. The effect of yohimbine can be explained by an increase in sympathetic tone.", "gemini": "Yohimbine đã được đề xuất để điều trị hạ huyết áp tư thế thần kinh; tuy nhiên, không có thử nghiệm có kiểm soát nào được thực hiện trên các mô hình hạ huyết áp tư thế thực nghiệm hoặc ở những bệnh nhân bị suy chức năng tự chủ. Mục đích của nghiên cứu hiện tại là so sánh tác dụng của yohimbine (0,05 mg/kg, tiêm tĩnh mạch [IV]) và giả dược (nước muối) trong một mô hình hạ huyết áp tư thế thần kinh mới thu được bằng cách cắt bỏ xoang động mạch chủ (SAD) ở chó gây mê bằng chloralose. Huyết áp, nhịp tim, nồng độ huyết tương noradrenaline và huyết áp tâm thu và biến đổi nhịp tim ngắn hạn (được tính trên các dải tần số thấp [40-50 MHz] và tần số cao [390-490 MHz]) được đo ở tư thế nằm ngửa và sau 10 phút nghiêng đầu lên 80 độ. Các loại thuốc được dùng theo cách ngẫu nhiên chéo mù đôi. Việc nghiêng đầu lên ở các động vật bình thường làm tăng huyết áp tâm trương (+12 +/- 4 mmHg), nhịp tim (+39 +/- 12 nhịp mỗi phút [bpm]), dải tần số thấp của huyết áp tâm thu và nồng độ huyết tương noradrenaline, mà không làm thay đổi huyết áp tâm thu hoặc biến đổi nhịp tim. Ở chó SAD, huyết áp tâm thu (-80 +/- 11 mmHg) và tâm trương (-43 +/- 4 mmHg) giảm rõ rệt trong vòng 1 phút sau khi dùng giả dược, mà không có sự thay đổi về nhịp tim, huyết áp tâm thu và biến đổi nhịp tim ngắn hạn và nồng độ huyết tương noradrenaline. Ở chó SAD, yohimbine (0,05 mg/kg, IV) làm chậm sự giảm huyết áp do nghiêng đầu lên, nhưng không làm thay đổi biên độ của nó. Những kết quả này cho thấy rằng, trong mô hình hạ huyết áp tư thế thu được bằng SAD, yohimbine, ở liều chọn lọc thụ thể alpha 2-adrenoceptor (0,05 mg/kg), làm chậm sự giảm huyết áp do nghiêng đầu lên. Tác dụng của yohimbine có thể được giải thích bằng cách tăng trương lực giao cảm."} +{"idx": 1950078, "en": "Bosentan (endothelin ETA/ETB antagonist), pinacidil (potassium channel opener) and nitroprusside (nitric oxide donor) were examined on isolated ring preparations of human intralobar pulmonary artery (3rd-5th generation, internal radius > or = 1 mm), rat main pulmonary artery (1st generation; internal radius > or = 1 mm) and rat intralobar pulmonary artery (3rd generation; internal radius 0.1-0.3 mm). The potency of endothelin-1 was the same in all three artery types. In human intralobar artery and rat main pulmonary artery, bosentan (3 and 10 microM) shifted the endothelin-1 concentration response curve to a higher concentration range (endothelin-1 concentration ratios, in human intralobar and rat main pulmonary artery, respectively: 3 microM bosentan, 4.5 and 8.1; 10 microM bosentan, 13.5 and 19.5), but caused no significant block of endothelin-1 in rat intralobar artery. The latter finding may be due to the reported presence of ETB receptors in rat intralobar arteries and the higher potency of bosentan on ETA than on ETB receptors. In contrast, the potencies of nitroprusside and pinacidil (relaxation of submaximal contractions to the thromboxane-mimetic, U46619) agreed on human and rat intralobar arteries but were 6 to 16-fold lower than on rat main pulmonary artery. We conclude that data obtained on pulmonary arteries from rats can be useful in predicting the effects of vasoactive drugs in human pulmonary arteries but selection of the most appropriate rat artery for study will depend on the drug group under investigation. For potassium channel openers and nitric oxide donors, good agreement between human and rat data will be found when using pulmonary arteries from the same anatomical location even though they differ markedly in size. In contrast, for endothelin antagonists, agreement is more likely to be found in arteries of comparable size, despite their different anatomical locations.", "gemini": "Bosentan (chất đối kháng thụ thể nội mô ETA/ETB), pinacidil (chất mở kênh kali) và nitroprusside (chất cung cấp nitric oxide) đã được kiểm tra trên các chế phẩm vòng cô lập của động mạch phổi thùy trong ở người (thế hệ thứ 3-5, bán kính bên trong > hoặc = 1 mm), động mạch phổi chính ở chuột (thế hệ thứ nhất; bán kính bên trong > hoặc = 1 mm) và động mạch phổi thùy trong ở chuột (thế hệ thứ 3; bán kính bên trong 0,1-0,3 mm). Hiệu lực của endothelin-1 là như nhau ở cả ba loại động mạch. Ở động mạch thùy trong ở người và động mạch phổi chính ở chuột, bosentan (3 và 10 microM) đã dịch chuyển đường cong đáp ứng nồng độ endothelin-1 sang phạm vi nồng độ cao hơn (tỷ lệ nồng độ endothelin-1, ở động mạch thùy trong ở người và động mạch phổi chính ở chuột, tương ứng: 3 microM bosentan, 4,5 và 8,1; 10 microM bosentan, 13,5 và 19,5), nhưng không gây ra sự chặn đáng kể endothelin-1 ở động mạch thùy trong ở chuột. Phát hiện sau có thể là do sự hiện diện đã được báo cáo của các thụ thể ETB ở động mạch thùy trong ở chuột và hiệu lực cao hơn của bosentan trên thụ thể ETA so với thụ thể ETB. Ngược lại, hiệu lực của nitroprusside và pinacidil (làm giãn các cơn co thắt dưới tối đa đối với chất bắt chước thromboxane, U46619) đồng ý ở động mạch thùy trong ở người và chuột nhưng thấp hơn 6 đến 16 lần so với động mạch phổi chính ở chuột. Chúng tôi kết luận rằng dữ liệu thu được trên động mạch phổi từ chuột có thể hữu ích trong việc dự đoán tác dụng của thuốc giãn mạch ở động mạch phổi ở người nhưng việc lựa chọn động mạch chuột thích hợp nhất để nghiên cứu sẽ phụ thuộc vào nhóm thuốc đang nghiên cứu. Đối với thuốc mở kênh kali và thuốc cung cấp nitric oxide, sẽ tìm thấy sự đồng ý tốt giữa dữ liệu ở người và chuột khi sử dụng động mạch phổi từ cùng một vị trí giải phẫu mặc dù chúng khác nhau đáng kể về kích thước. Ngược lại, đối với thuốc đối kháng endothelin, sự đồng ý có nhiều khả năng xảy ra ở các động mạch có kích thước tương đương, mặc dù vị trí giải phẫu của chúng khác nhau."} +{"idx": 1950079, "en": "Understanding the basic physiology of endocrinology and metabolism is important for the safe conduct of anesthesia of patients with endocrine disease. Full endocrine assessment and considered interpretation of the results is essential before anesthesia and surgery. In this chapter, we have covered the background physiology and the basic investigations that may be required to diagnose and direct treatment in order to optimize perioperative care.", "gemini": "Hiểu được sinh lý học cơ bản của nội tiết và chuyển hóa là rất quan trọng để thực hiện an toàn gây mê cho bệnh nhân mắc bệnh nội tiết. Đánh giá nội tiết đầy đủ và giải thích kết quả được cân nhắc là rất cần thiết trước khi gây mê và phẫu thuật. Trong chương này, chúng tôi đã đề cập đến sinh lý học nền và các xét nghiệm cơ bản có thể cần thiết để chẩn đoán và chỉ đạo điều trị nhằm tối ưu hóa chăm sóc quanh phẫu thuật."} +{"idx": 1950080, "en": "Transsphenoidal operations for pituitary tumors have dramatically decreased the morbidity of pituitary surgery in a group of patients with many potential anesthetic problems. Although a good preoperative endocrine workup and anticipation of problems are the key to ensuring a good outcome, it remains a challenge to the \"art\" of neuroanesthesia to produce a smooth and safe anesthetic.", "gemini": "Các phẫu thuật qua hố bướm đối với khối u tuyến yên đã làm giảm đáng kể tỷ lệ mắc bệnh của phẫu thuật tuyến yên ở một nhóm bệnh nhân có nhiều vấn đề gây mê tiềm ẩn. Mặc dù việc chuẩn bị nội tiết trước phẫu thuật tốt và dự đoán các vấn đề là chìa khóa để đảm bảo kết quả tốt, nhưng vẫn là một thách thức đối với \"nghệ thuật\" gây mê thần kinh để tạo ra một loại thuốc gây mê an toàn và suôn sẻ."} +{"idx": 1950081, "en": "Preoperative preparation for the patient with adrenal disorders is directed toward restoring the intravascular volume and the electrolyte concentrations to normal. Hypertension and hypokalemia may be controlled by restricting sodium intake and administration of the aldosterone antagonist spironolactone. Patients taking steroids preoperatively must be adequately replaced throughout the perioperative phase at times of maximum stress.", "gemini": "Việc chuẩn bị trước phẫu thuật cho bệnh nhân mắc các rối loạn tuyến thượng thận nhằm mục đích phục hồi thể tích nội mạch và nồng độ điện giải trở lại bình thường. Tăng huyết áp và hạ kali máu có thể được kiểm soát bằng cách hạn chế lượng natri nạp vào và dùng thuốc đối kháng aldosterone spironolactone. Bệnh nhân dùng steroid trước phẫu thuật phải được thay thế đầy đủ trong suốt giai đoạn quanh phẫu thuật tại thời điểm căng thẳng cao nhất."} +{"idx": 1950082, "en": "The management of patients with pheochromocytoma remains a challenge for the anesthetist despite the advent of new drugs and techniques. Safe anesthesia still depends on adequate preoperative preparation and assessment together with careful monitoring during the perioperative period.", "gemini": "Việc điều trị cho bệnh nhân mắc u tế bào ưa crôm vẫn là một thách thức đối với bác sĩ gây mê mặc dù đã có những loại thuốc và kỹ thuật mới. Gây mê an toàn vẫn phụ thuộc vào việc chuẩn bị và đánh giá trước phẫu thuật đầy đủ cùng với việc theo dõi cẩn thận trong suốt giai đoạn quanh phẫu thuật."} +{"idx": 1950083, "en": "BACKGROUND The prevalence of psychiatric disorders in nursing homes, which has not been studied before in Nigeria, is the subject of this cross-sectional study. METHOD Psychiatric disorders were assessed in 23 of the 29 residents of the two nursing homes in Lagos and diagnoses based on DSM-III-R criteria (APA, 1987) and AGECAT (Copeland et al., 1986) were made independently. RESULTS The commonest diagnoses were dementia in 11 out of 23 residents and depression in four residents. Overall prevalence rate of psychiatric disorders was 74%. CONCLUSION The present prevalence rate of psychiatric disorders is similar to those reported in similar institutions in the industrialized societies. This has implications for the care of the elderly in a non-industrialized society.", "gemini": "BỐI CẢNH Tỷ lệ mắc các rối loạn tâm thần ở viện dưỡng lão, chưa từng được nghiên cứu trước đây ở Nigeria, là chủ đề của nghiên cứu cắt ngang này. PHƯƠNG PHÁP Các rối loạn tâm thần đã được đánh giá ở 23 trong số 29 cư dân của hai viện dưỡng lão ở Lagos và chẩn đoán dựa trên tiêu chuẩn DSM-III-R (APA, 1987) và AGECAT (Copeland et al., 1986) được thực hiện độc lập. KẾT QUẢ Các chẩn đoán phổ biến nhất là chứng mất trí ở 11 trên 23 cư dân và trầm cảm ở bốn cư dân. Tỷ lệ mắc chung của các rối loạn tâm thần là 74%. KẾT LUẬN Tỷ lệ mắc các rối loạn tâm thần hiện tại tương tự như những tỷ lệ được báo cáo tại các cơ sở tương tự ở các xã hội công nghiệp. Điều này có ý nghĩa đối với việc chăm sóc người cao tuổi trong xã hội chưa công nghiệp."} +{"idx": 1950084, "en": "OBJECTIVES To evaluate whether the CAGE, MAST-G or an abnormally high MCV are effective screening instruments for alcohol misuse and if not to devise a new instrument. DESIGN Questionnaire survey of randomly selected patients. SETTING Acute admission wards of two district general hospitals. PERIOD 9 months. SUBJECTS Randomly selected patients, aged 65 and over, admitted as emergencies. MAIN OUTCOME MEASURES Excessive alcohol intake, alcohol dependence. RESULTS The sensitivities of the CAGE, MAST-G and an abnormally high MCV for excess alcohol intake and alcohol abuse are low, ranging from 0.13 to 0.54. CONCLUSION The results confirm previous UK studies which indicate that the CAGE and an abnormally high MCV are poor screening instruments for alcohol misuse in older patients and also indicate that the MAST-G is an insensitive screening instrument. A new two-step instrument with five questions in total was generated. This requires further validation.", "gemini": "MỤC TIÊU Đánh giá CAGE, MAST-G hoặc MCV cao bất thường có phải là công cụ sàng lọc hiệu quả đối với việc sử dụng rượu quá mức hay không và nếu không thì hãy đưa ra một công cụ mới. THIẾT KẾ Khảo sát bảng câu hỏi của những bệnh nhân được chọn ngẫu nhiên. BỐI CẢNH Các khoa cấp cứu của hai bệnh viện đa khoa quận. THỜI GIAN 9 tháng. ĐỐI TƯỢNG Những bệnh nhân được chọn ngẫu nhiên, từ 65 tuổi trở lên, được đưa vào cấp cứu. CÁC BIỆN PHÁP ĐO LƯỜNG KẾT QUẢ CHÍNH Uống rượu quá mức, nghiện rượu. KẾT QUẢ Độ nhạy của CAGE, MAST-G và MCV cao bất thường đối với việc uống rượu quá mức và lạm dụng rượu là thấp, dao động từ 0,13 đến 0,54. KẾT LUẬN Các kết quả xác nhận các nghiên cứu trước đây ở Vương quốc Anh chỉ ra rằng CAGE và MCV cao bất thường là các công cụ sàng lọc kém đối với việc sử dụng rượu quá mức ở những bệnh nhân lớn tuổi và cũng chỉ ra rằng MAST-G là một công cụ sàng lọc không nhạy. Một công cụ hai bước mới với tổng cộng năm câu hỏi đã được tạo ra. Điều này đòi hỏi phải xác nhận thêm."} +{"idx": 1950085, "en": "OBJECTIVE The principal aim of this pilot study was to evaluate the performance of a Gujarati version of the MMSE as a screening instrument for dementia. The effect of ethnicity on MMSE performance was also examined. DESIGN Two-stage cross-cultural survey. SETTING Elderly immigrant Gujarati and British-born white communities in Leicester. SUBJECTS First stage: 149 Gujaratis and 148 whites. Second stage: 27 Gujaratis and 42 whites. MEASURES English and Gujarati versions of the MMSE, validated against clinical diagnosis following psychiatric interview (ICD-10 criteria). RESULTS Mean MMSE scores were lower in the Gujarati group, but most of this difference was due to the effects of age, education and visual impairment. Ethnic group had an independent effect on three orientation items; when these were omitted, there was no difference in the mean MMSE scores of the groups after adjustment for age, education and visual impairment. The MMSE performed comparably in both groups as a screen for moderate-severe dementia, but was less efficient at detecting milder and less certain cases in the Gujarati group. The estimated prevalence of confirmed dementia was higher in the Gujarati group, but this was not statistically significant. CONCLUSIONS This Gujarati version of the MMSE performed adequately as a screen for dementia in this immigrant community population. Further evaluation of its performance is required in larger community samples, clinical samples and in native Indian Gujaratis.", "gemini": "MỤC TIÊU Mục tiêu chính của nghiên cứu thí điểm này là đánh giá hiệu quả của phiên bản MMSE tiếng Gujarati như một công cụ sàng lọc chứng mất trí. Hiệu ứng của dân tộc đối với hiệu suất của MMSE cũng được kiểm tra. THIẾT KẾ Khảo sát liên văn hóa hai giai đoạn. BỐI CẢNH Cộng đồng người Gujarati nhập cư lớn tuổi và người da trắng sinh ra ở Anh tại Leicester. ĐỐI TƯỢNG Giai đoạn đầu: 149 người Gujarati và 148 người da trắng. Giai đoạn hai: 27 người Gujarati và 42 người da trắng. CÁC BIỆN PHÁP Các phiên bản MMSE tiếng Anh và tiếng Gujarati, được xác nhận so với chẩn đoán lâm sàng sau phỏng vấn tâm thần (tiêu chuẩn ICD-10). KẾT QUẢ Điểm MMSE trung bình thấp hơn ở nhóm người Gujarati, nhưng hầu hết sự khác biệt này là do ảnh hưởng của tuổi tác, trình độ học vấn và khiếm thị. Nhóm dân tộc có ảnh hưởng độc lập đến ba mục định hướng; khi những mục này bị bỏ qua, không có sự khác biệt về điểm MMSE trung bình của các nhóm sau khi điều chỉnh tuổi tác, trình độ học vấn và khiếm thị. MMSE hoạt động tương đương ở cả hai nhóm như một công cụ sàng lọc chứng mất trí vừa đến nặng, nhưng kém hiệu quả hơn trong việc phát hiện các trường hợp nhẹ hơn và không chắc chắn hơn ở nhóm người Gujarati. Tỷ lệ mắc chứng mất trí được xác nhận ước tính cao hơn ở nhóm người Gujarati, nhưng điều này không có ý nghĩa thống kê. KẾT LUẬN Phiên bản MMSE tiếng Gujarati này hoạt động đầy đủ như một công cụ sàng lọc chứng mất trí trong cộng đồng dân nhập cư này. Cần đánh giá thêm hiệu suất của nó trong các mẫu cộng đồng lớn hơn, mẫu lâm sàng và ở người Gujarati bản địa Ấn Độ."} +{"idx": 1950086, "en": "OBJECTIVE This study aimed to: (i) replicate previous findings that women experience more strain and distress than men when caring for dementing spouses; (ii) explore what factors underlie this sex difference. DESIGN Two closely matched subsamples of spouse carers, husbands and wives respectively, were selected and the two groups compared. SETTING An urban psychogeriatric service in the UK. SUBJECTS 48 spouses of referred dementia cases. MEASURES Mental health was rated by the General Health Questionnaire, the Strain Scale and the Life Satisfaction Index. Various factors related to caregiving were assessed by the Problem Checklist, and a specially devised instrument to record caregiving task involvement, formal/informal support, objective burden, satisfaction with supports and attitudes to the relationship and to caregiving. RESULTS The wives' levels of strain and morale were significantly worse than husbands'. There were qualitative differences between husbands and wives in terms of caregiving tasks undertaken. Wives tolerated dementia sufferers depending on them less well. Wives were also more likely to wish to leave caregiving to someone else and cited more reasons for giving up. Subjects of both sexes displayed a strong tendency to view women as generally better suited to the caregiving role, and wives felt a greater obligation to care. CONCLUSIONS It is concluded that such attitudinal or social factors are the chief determinants of wives' higher levels of strain and distress.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm mục đích: (i) lặp lại những phát hiện trước đây rằng phụ nữ trải qua nhiều căng thẳng và đau khổ hơn nam giới khi chăm sóc những người bạn đời bị mất trí; (ii) khám phá những yếu tố nào là nguyên nhân gây ra sự khác biệt về giới tính này. THIẾT KẾ Hai nhóm phụ mẫu phù hợp chặt chẽ gồm những người chăm sóc bạn đời, lần lượt là chồng và vợ, đã được lựa chọn và hai nhóm được so sánh. BỐI CẢNH Một dịch vụ tâm thần học ở Anh. ĐỐI TƯỢNG 48 người bạn đời của các trường hợp mất trí được giới thiệu. CÁC BIỆN PHÁP Sức khỏe tâm thần được đánh giá bằng Bản câu hỏi sức khỏe chung, Thang đo căng thẳng và Chỉ số hài lòng cuộc sống. Các yếu tố khác nhau liên quan đến việc chăm sóc được đánh giá bằng Danh sách kiểm tra vấn đề và một công cụ được thiết kế đặc biệt để ghi lại sự tham gia vào nhiệm vụ chăm sóc, hỗ trợ chính thức/phi chính thức, gánh nặng khách quan, sự hài lòng với các hỗ trợ và thái độ đối với mối quan hệ và việc chăm sóc. KẾT QUẢ Mức độ căng thẳng và tinh thần của những người vợ tệ hơn đáng kể so với những người chồng. Có sự khác biệt về mặt định tính giữa chồng và vợ về các nhiệm vụ chăm sóc được thực hiện. Những người vợ ít chịu đựng được những người mắc chứng mất trí phụ thuộc vào họ. Những người vợ cũng có nhiều khả năng muốn giao việc chăm sóc cho người khác và nêu ra nhiều lý do hơn để từ bỏ. Những đối tượng ở cả hai giới đều có xu hướng mạnh mẽ coi phụ nữ nói chung phù hợp hơn với vai trò chăm sóc, và những người vợ cảm thấy có nghĩa vụ chăm sóc nhiều hơn. KẾT LUẬN Có thể kết luận rằng các yếu tố thái độ hoặc xã hội như vậy là yếu tố chính quyết định mức độ căng thẳng và đau khổ cao hơn của những người vợ."} +{"idx": 1950087, "en": "The observed mortality of 397 elderly patients with organic brain disorder, recruited to the Salford Psychiatric Case Register between 1 January 1968 and 31 December 1975 and followed up by record linkage to 31 December 1985, was compared with expected mortality. The overall SMR was 1.91. SMRs were significantly raised for all age groups beyond 65 years, being highest in the younger age-bands and declining almost linearly with age. SMRs were elevated for both men and women, during all calendar periods, for incident and prevalent cases, and throughout the whole follow-up period, being highest in the first year. While social class variations may account for up to 9% of the excess deaths, marital status and ethnic differences had little effect.", "gemini": "Tỷ lệ tử vong quan sát được của 397 bệnh nhân cao tuổi mắc chứng rối loạn não bộ hữu cơ, được tuyển dụng vào Sổ đăng ký ca bệnh tâm thần Salford trong khoảng thời gian từ ngày 1 tháng 1 năm 1968 đến ngày 31 tháng 12 năm 1975 và được theo dõi bằng cách liên kết hồ sơ đến ngày 31 tháng 12 năm 1985, đã được so sánh với tỷ lệ tử vong dự kiến. Tỷ lệ SMR chung là 1,91. Tỷ lệ SMR tăng đáng kể đối với tất cả các nhóm tuổi trên 65, cao nhất ở các nhóm tuổi trẻ hơn và giảm gần như tuyến tính theo độ tuổi. Tỷ lệ SMR tăng cao đối với cả nam và nữ, trong tất cả các giai đoạn lịch, đối với các trường hợp mới phát và hiện mắc, và trong suốt toàn bộ thời gian theo dõi, cao nhất là trong năm đầu tiên. Trong khi các biến thể về giai cấp xã hội có thể chiếm tới 9% số ca tử vong quá mức, thì tình trạng hôn nhân và khác biệt về dân tộc có ít ảnh hưởng."} +{"idx": 1950088, "en": "OBJECTIVES To investigate whether the presence of hippocampal atrophy (HCA) on MRI in Alzheimer's disease (AD) leads to a more rapid decline in cognitive function. To investigate whether cognitively unimpaired controls and depressed subjects with HCA are at higher risk than those without HCA of developing dementia. DESIGN A prospective follow-up of subjects from a previously reported MRI study. SETTING Melbourne, Australia. PARTICIPANTS Five controls with HCA and five age-matched controls without HCA, seven depressed subjects with HCA and seven without HCA, and 12 subjects with clinically diagnosed probable AD with HCA and 12 without HCA were studied. They were followed up at approximately 2 years with repeat cognitive testing, blind to initial diagnosis and MRI result. MEASURES HCA was rated by two radiologists blind to cognitive test score results. Cognitive assessment was by the Cambridge Cognitive Examination (CAMCOG). RESULTS No significant differences in rate of cognitive decline, mortality or progression to dementia were found between subjects with or without HCA. CONCLUSIONS HCA was not found to be a predictor of subsequent cognitive decline in this series.", "gemini": "MỤC TIÊU Để điều tra xem sự hiện diện của teo hồi hải mã (HCA) trên MRI trong bệnh Alzheimer (AD) có dẫn đến suy giảm nhanh hơn về chức năng nhận thức hay không. Để điều tra xem những đối tượng đối chứng không suy giảm nhận thức và những người trầm cảm mắc HCA có nguy cơ mắc chứng mất trí cao hơn những người không mắc HCA hay không. THIẾT KẾ Theo dõi triển vọng các đối tượng từ một nghiên cứu MRI đã được báo cáo trước đó. BỐI CẢNH Melbourne, Úc. NGƯỜI THAM GIA Năm đối chứng mắc HCA và năm đối chứng không mắc HCA cùng độ tuổi, bảy đối tượng trầm cảm mắc HCA và bảy đối tượng không mắc HCA, và 12 đối tượng được chẩn đoán lâm sàng là có khả năng mắc AD mắc HCA và 12 đối tượng không mắc HCA đã được nghiên cứu. Họ được theo dõi sau khoảng 2 năm với bài kiểm tra nhận thức lặp lại, không biết chẩn đoán ban đầu và kết quả MRI. CÁC BIỆN PHÁP HCA được đánh giá bởi hai bác sĩ vô tuyến không biết kết quả kiểm tra nhận thức. Đánh giá nhận thức được thực hiện bằng Bài kiểm tra nhận thức Cambridge (CAMCOG). KẾT QUẢ Không có sự khác biệt đáng kể về tốc độ suy giảm nhận thức, tử vong hoặc tiến triển thành mất trí giữa những đối tượng mắc HCA hoặc không mắc HCA. KẾT LUẬN HCA không được tìm thấy là yếu tố dự báo suy giảm nhận thức sau đó trong loạt nghiên cứu này."} +{"idx": 1950089, "en": "OBJECTIVES To determine the response to treatment of different subgroups within a sample of physically ill elderly depressed patients. DESIGN Acute geriatric medical inpatients with depression, randomly assigned to an 8-week double-blind placebo-controlled trial of fluoxetine. MAIN OUTCOME MEASURE Response rate as defined by the 17-item Hamilton Depression Rating Scale. RESULTS Data were analysed in subgroups according to diagnosed physical problems and concomitant medication. A logistic regression analysis was performed to identify subgroups valid for separate analysis. Those reaching at least 5 weeks of treatment showed a significant improvement compared with the placebo group if they had serious life-threatening disease, cerebrovascular disease, were not demented, or were either on no analgesics or on analgesics stronger than paracetamol. CONCLUSION While the response to treatment in these subgroups was encouraging, general physicians must not be led to believe that the answer to depression lies only in pharmacological intervention, just as the belief that the risk-benefit ratio of such treatment where indicated is too high must be discouraged.", "gemini": "MỤC TIÊU Xác định đáp ứng với điều trị của các phân nhóm khác nhau trong một mẫu bệnh nhân cao tuổi bị trầm cảm do bệnh lý. THIẾT KẾ Bệnh nhân nội trú cấp tính khoa lão khoa mắc chứng trầm cảm, được chỉ định ngẫu nhiên vào thử nghiệm đối chứng giả dược có kiểm soát trong 8 tuần bằng fluoxetine. BIỆN PHÁP ĐO KẾT QUẢ CHÍNH Tỷ lệ đáp ứng được xác định theo Thang đánh giá trầm cảm Hamilton 17 mục. KẾT QUẢ Dữ liệu được phân tích theo các phân nhóm theo các vấn đề sức khỏe được chẩn đoán và thuốc đi kèm. Một phân tích hồi quy logistic đã được thực hiện để xác định các phân nhóm hợp lệ cho phân tích riêng biệt. Những người đạt ít nhất 5 tuần điều trị cho thấy sự cải thiện đáng kể so với nhóm giả dược nếu họ mắc bệnh đe dọa tính mạng nghiêm trọng, bệnh mạch máu não, không bị mất trí hoặc không dùng thuốc giảm đau hoặc dùng thuốc giảm đau mạnh hơn paracetamol. KẾT LUẬN Mặc dù đáp ứng với điều trị ở những phân nhóm này là đáng khích lệ, nhưng các bác sĩ đa khoa không nên tin rằng câu trả lời cho chứng trầm cảm chỉ nằm ở can thiệp dược lý, cũng như niềm tin rằng tỷ lệ rủi ro - lợi ích của phương pháp điều trị như vậy khi được chỉ định là quá cao phải bị ngăn chặn."} +{"idx": 1950090, "en": "Chronic pancreatitis is a painful disease, ranging between uncomplicated courses with recurrent pain but longer pain-free intervals and complicated courses with constant intractable pain or frequent severe attacks of pain. Several factors, including intrapancreatic and extrapancreatic abnormalities, contribute to pain in chronic pancreatitis and their relative contribution may vary from patient to patient. The natural course of pain is modified by the use of analgesic drugs, by interventional treatment modalities, and by operative procedures. Since standards for the evaluation are lacking, treatment results have been evaluated following very different scales and protocols. Interdisciplinary controlled clinical trials comparing interventional and operative treatment are not yet available. For future studies, it seems necessary to recruit patients following a standardized clinically based staging system, to assess treatment results following standardized measures for pain and quality of life. For these purposes, such a staging system is proposed, and the application of the European Organization for Research and Treatment of Cancer (EORTC) quality of life (QLQ) questionnaire is suggested.", "gemini": "Viêm tụy mạn tính là một căn bệnh gây đau đớn, dao động giữa các diễn biến không biến chứng với cơn đau tái phát nhưng khoảng không đau kéo dài hơn và các diễn biến phức tạp với cơn đau dai dẳng không thể chịu đựng được hoặc các cơn đau dữ dội thường xuyên. Một số yếu tố, bao gồm các bất thường trong tụy và ngoài tụy, góp phần gây đau trong viêm tụy mạn tính và mức độ đóng góp tương đối của chúng có thể khác nhau tùy từng bệnh nhân. Diễn biến tự nhiên của cơn đau bị thay đổi do việc sử dụng thuốc giảm đau, phương thức điều trị can thiệp và các thủ thuật phẫu thuật. Do thiếu các tiêu chuẩn đánh giá nên kết quả điều trị đã được đánh giá theo các thang điểm và giao thức rất khác nhau. Các thử nghiệm lâm sàng có đối chứng liên ngành so sánh điều trị can thiệp và điều trị phẫu thuật vẫn chưa có. Đối với các nghiên cứu trong tương lai, có vẻ cần thiết phải tuyển dụng bệnh nhân theo hệ thống phân giai dựa trên lâm sàng đã chuẩn hóa, để đánh giá kết quả điều trị theo các biện pháp chuẩn hóa về cơn đau và chất lượng cuộc sống. Vì những mục đích này, một hệ thống phân giai như vậy được đề xuất và việc áp dụng bảng câu hỏi về chất lượng cuộc sống (QLQ) của Tổ chức nghiên cứu và điều trị ung thư châu Âu (EORTC) được gợi ý."} +{"idx": 1950091, "en": "CONCLUSION A dosage of 300 mg/d of allopurinol was not effective in reducing pain or improving activities of daily living in chronic pancreatitis. BACKGROUND Allopurinol prevents the generation of oxygen-derived free radicals by inhibiting xanthine oxidase. The purpose of this study was to determine whether allopurinol is effective in reducing pain of chronic pancreatitis. METHODS Thirteen patients with chronic pancreatitis who were experiencing abdominal pain requiring medication at least three times each week entered a randomized, double-blind, two-period crossover clinical trial. Patients evaluated their pain daily using a categorical pain intensity scale, numeric pain intensity scale, and a visual analog scale, and weekly completed a McGill Pain Questionnaire and activities of daily living (ADL) questionnaire. RESULTS The mean baseline score of pain was approx 50% of most severe pain in all scoring systems. There was no significant decrease in pain associated with allopurinol compared to the placebo (p = 0.24-0.75). In addition, there was no benefit in terms of ADL score associated with allopurinol compared with placebo (p = 0.32). Mean uric acid level was decreased by 1.15 mg/dL while patients were taking allopurinol, compared to when they were taking placebo (p = 0.007).", "gemini": "KẾT LUẬN Liều allopurinol 300 mg/ngày không có hiệu quả trong việc giảm đau hoặc cải thiện các hoạt động sinh hoạt hàng ngày ở bệnh nhân viêm tụy mạn tính. LÝ DO Allopurinol ngăn ngừa sự tạo ra các gốc tự do có nguồn gốc từ oxy bằng cách ức chế xanthine oxidase. Mục đích của nghiên cứu này là xác định xem allopurinol có hiệu quả trong việc giảm đau do viêm tụy mạn tính hay không. PHƯƠNG PHÁP Mười ba bệnh nhân viêm tụy mạn tính bị đau bụng và phải dùng thuốc ít nhất ba lần mỗi tuần đã tham gia thử nghiệm lâm sàng chéo hai giai đoạn, ngẫu nhiên, mù đôi. Bệnh nhân tự đánh giá cơn đau hàng ngày bằng thang cường độ đau theo danh mục, thang cường độ đau theo số và thang tương tự thị giác, và hoàn thành hàng tuần một bảng câu hỏi về cơn đau McGill và bảng câu hỏi về các hoạt động sinh hoạt hàng ngày (ADL). KẾT QUẢ Điểm đau cơ bản trung bình là khoảng 50% cơn đau dữ dội nhất trong tất cả các hệ thống chấm điểm. Không có sự giảm đau đáng kể nào liên quan đến allopurinol so với giả dược (p = 0,24-0,75). Ngoài ra, không có lợi ích nào về điểm ADL liên quan đến allopurinol so với giả dược (p = 0,32). Nồng độ axit uric trung bình giảm 1,15 mg/dL khi bệnh nhân dùng allopurinol, so với khi dùng giả dược (p = 0,007)."} +{"idx": 1950092, "en": "CONCLUSION In normal adult rats, administration of a low dose of loxiglumide for 7 d had no significant effect on exocrine and endocrine pancreatic function, whereas a high dose of loxiglumide decreased pancreatic enzyme output without inducing insulin resistance and diabetes mellitus. BACKGROUND There is a possibility that chronic administration of cholecystokinin receptor antagonists not only inhibits the growth of the pancreas but also alters exocrine and endocrine pancreatic function. METHODS Loxiglumide at a dose of 50, 100, or 200 mg/kg body weight, or the same volume of saline, was given by an orogastric tube twice daily for 7 d (13 successive times). Biochemical and functional changes were determined on d 8 at 24 h after the last administration of loxiglumide and 18 h fasting. Pancreatic exocrine and endocrine function was simultaneously determined following an intravenous injection of a mixed solution of 0.5 g/kg body weight glucose plus 100 ng/kg body weight cerulein. RESULTS Pancreatic weight and protein content were dose-dependently decreased by loxiglumide, whereas DNA content was decreased only by the highest dose of loxiglumide. Loxiglumide caused dose-dependent decreases in pancreatic fluid and protein output. Total pancreatic insulin content in rats treated with loxiglumide was not significantly different from that in the control rats. However, insulin concentration relative to DNA content was significantly increased in rats treated with 200 mg/kg body weight loxiglumide compared with that in other groups of rats. Glucose-stimulated insulin release was significantly low in rats treated with the highest dose of loxiglumide compared with that in other groups of rats, although there was no difference of serum glucose concentrations among these four groups of rats.", "gemini": "KẾT LUẬN Ở những con chuột trưởng thành bình thường, việc dùng một liều thấp loxiglumide trong 7 ngày không có tác dụng đáng kể đến chức năng ngoại tiết và nội tiết của tuyến tụy, trong khi một liều cao loxiglumide làm giảm lượng enzyme tụy tiết ra mà không gây kháng insulin và đái tháo đường. LÝ DO Có khả năng là việc dùng lâu dài các chất đối kháng thụ thể cholecystokinin không chỉ ức chế sự phát triển của tuyến tụy mà còn làm thay đổi chức năng ngoại tiết và nội tiết của tuyến tụy. PHƯƠNG PHÁP Loxiglumide với liều 50, 100 hoặc 200 mg/kg trọng lượng cơ thể, hoặc cùng thể tích dung dịch muối, được dùng qua ống thông dạ dày hai lần mỗi ngày trong 7 ngày (13 lần liên tiếp). Các thay đổi về mặt sinh hóa và chức năng được xác định vào ngày thứ 8, tức là 24 giờ sau lần dùng loxiglumide cuối cùng và nhịn ăn 18 giờ. Chức năng ngoại tiết và nội tiết của tuyến tụy được xác định đồng thời sau khi tiêm tĩnh mạch hỗn hợp gồm 0,5 g/kg trọng lượng cơ thể glucose cộng với 100 ng/kg trọng lượng cơ thể cerulein. KẾT QUẢ Trọng lượng và hàm lượng protein của tuyến tụy giảm phụ thuộc vào liều dùng loxiglumide, trong khi hàm lượng DNA chỉ giảm ở liều loxiglumide cao nhất. Loxiglumide gây ra tình trạng giảm tiết dịch tụy và protein phụ thuộc vào liều dùng. Tổng lượng insulin trong tụy ở những con chuột được điều trị bằng loxiglumide không khác biệt đáng kể so với những con chuột đối chứng. Tuy nhiên, nồng độ insulin so với hàm lượng DNA tăng đáng kể ở những con chuột được điều trị bằng loxiglumide 200 mg/kg trọng lượng cơ thể so với những con chuột trong các nhóm khác. Sự giải phóng insulin được kích thích bởi glucose thấp đáng kể ở những con chuột được điều trị bằng liều loxiglumide cao nhất so với những con chuột trong các nhóm khác, mặc dù không có sự khác biệt về nồng độ glucose trong huyết thanh giữa bốn nhóm chuột này."} +{"idx": 1950093, "en": "Four cases of pancreatic cancer associated with pancreatic stones were investigated clinicopathologically. In Case 1, pancreatic cancer was diagnosed 2 yr 9 mo after the diagnosis of pancreatolithiasis. Numerous large stones were present throughout the pancreas, and stones in the resected specimen were embedded in the tumor. Chronic inflammation, including inflammatory infiltration and fibrosis, were found around the pancreatic duct where the stones were present, both within and adjacent to the tumor, suggesting that chronic pancreatitis had been present for a protracted period. In Cases 2-4, a few small free stones were found in the dilated pancreatic ducts and a cyst upstream from the tumor, but there were no stones noted within the tumor. Microscopically, mild atrophy of the pancreatic parenchyma and fibrosis were seen. In each of these three cases, it was considered that the pancreatic cancer was the primary event, with the pancreatic stones arising as a secondary process. The pancreatic cancers associated with pancreatic stones were classified into two groups, one with pancreatic stones preceding the pancreatic cancer and the other three cases in which the reverse occurred.", "gemini": "Bốn trường hợp ung thư tụy liên quan đến sỏi tụy đã được nghiên cứu về mặt lâm sàng và bệnh học. Trong Trường hợp 1, ung thư tụy được chẩn đoán 2 năm 9 tháng sau khi chẩn đoán sỏi tụy. Có nhiều viên sỏi lớn xuất hiện khắp tụy và các viên sỏi trong mẫu vật cắt bỏ được nhúng vào khối u. Viêm mãn tính, bao gồm thâm nhiễm viêm và xơ hóa, được tìm thấy xung quanh ống tụy nơi có các viên sỏi, cả bên trong và bên cạnh khối u, cho thấy viêm tụy mãn tính đã tồn tại trong một thời gian dài. Trong các trường hợp 2-4, một vài viên sỏi nhỏ tự do được tìm thấy trong các ống tụy giãn và một nang ở thượng nguồn khối u, nhưng không có sỏi nào được ghi nhận trong khối u. Soi kính hiển vi cho thấy teo nhẹ nhu mô tụy và xơ hóa. Trong mỗi ba trường hợp này, người ta cho rằng ung thư tụy là sự kiện chính, với sỏi tụy phát sinh như một quá trình thứ phát. Ung thư tụy liên quan đến sỏi tụy được phân thành hai nhóm, một nhóm có sỏi tụy trước ung thư tụy và ba trường hợp còn lại xảy ra ngược lại."} +{"idx": 1950094, "en": "CONCLUSION Cu/Zn-SOD is present in pancreatic juice and tissue. Immunohistochemical studies reveal a localization of this enzyme in islet, duct, and centroacinar cells, but to a much lower extent in pancreatic acinar cells. BACKGROUND It is generally accepted that oxygen radicals are involved in the pathogenesis of acute and chronic pancreatitis. An imbalance of radical-generating and radical-scavenging processes is thought to lead to the damage of pancreatic acinar cells that initiate the autodigestion of the whole organ. METHODS We investigated the distribution pattern of the cytosolic radical-scavenging enzyme, copper/zinc-superoxide dismutase (Cu/Zn-SOD), in pancreatic juice and tissue. In patients with chronic pancreatitis or pancreatic malignancies, Cu-Zn-SOD was quantitated in different fractions of pancreatic juice by means of an enzyme immunoassay using two Cu/Zn-SOD-specific monoclonal antibodies. Cryostat or paraffin sections of pancreatic tissue were analyzed by immunohistochemical techniques. RESULTS We found this enzyme to be present in the first secretin-triggered fraction of endoscopically obtained pancreatic juice in concentrations similar to serum. In contrast, after cholecystokinin stimulation, only low levels could be found in pancreatic juice, indicating that this enzyme is not actively secreted. Interestingly, pancreatic juice of patients with chronic pancreatitis or pancreas tumor contained higher levels (25-29 ng/mL) of Cu/Zn-SOD than juice of controls without pancreatic diseases (15 ng/mL). Immunohistochemical studies of Cu/Zn-SOD in pancreatic tissue revealed a more intense staining of duct cells, islet cells, and centroacinar cells, whereas acinar cells showed almost no staining for Cu/Zn-SOD.", "gemini": "KẾT LUẬN Cu/Zn-SOD có trong dịch tụy và mô. Các nghiên cứu miễn dịch mô hóa học cho thấy sự định vị của enzyme này trong các tế bào đảo, ống và trung tâm nang, nhưng ở mức độ thấp hơn nhiều trong các tế bào tuyến tụy. BỐI CẢNH Người ta thường chấp nhận rằng các gốc oxy tham gia vào quá trình sinh bệnh của viêm tụy cấp và mãn tính. Sự mất cân bằng giữa các quá trình tạo gốc và loại bỏ gốc được cho là dẫn đến tổn thương các tế bào tuyến tụy khởi phát quá trình tự tiêu hóa của toàn bộ cơ quan. PHƯƠNG PHÁP Chúng tôi đã nghiên cứu mô hình phân bố của enzyme dọn gốc tế bào, đồng/kẽm-superoxide dismutase (Cu/Zn-SOD), trong dịch tụy và mô. Ở những bệnh nhân bị viêm tụy mãn tính hoặc ung thư tụy, Cu-Zn-SOD được định lượng trong các phân đoạn khác nhau của dịch tụy bằng phương pháp xét nghiệm miễn dịch enzym sử dụng hai kháng thể đơn dòng đặc hiệu Cu/Zn-SOD. Các lát cắt đông lạnh hoặc nhuộm parafin của mô tụy được phân tích bằng các kỹ thuật miễn dịch mô hóa học. KẾT QUẢ Chúng tôi phát hiện ra rằng enzyme này có trong phân đoạn đầu tiên do secretin kích hoạt của dịch tụy thu được bằng nội soi ở nồng độ tương tự như huyết thanh. Ngược lại, sau khi kích thích cholecystokinin, chỉ có thể tìm thấy nồng độ thấp trong dịch tụy, cho thấy enzyme này không được tiết ra tích cực. Điều thú vị là dịch tụy của bệnh nhân viêm tụy mãn tính hoặc khối u tụy chứa nồng độ Cu/Zn-SOD cao hơn (25-29 ng/mL) so với dịch của những người đối chứng không mắc bệnh tụy (15 ng/mL). Các nghiên cứu miễn dịch mô hóa học về Cu/Zn-SOD trong mô tụy cho thấy nhuộm đậm hơn ở các tế bào ống, tế bào đảo và tế bào trung tâm nang, trong khi các tế bào tuyến hầu như không nhuộm Cu/Zn-SOD."} +{"idx": 1950095, "en": "CONCLUSION These data show that pure pancreatic juice of AICP patients has a markedly defective antibacterial activity. This finding might be of potential clinical interest in the understanding of the pathophysiology of the disease. BACKGROUND The aim of the present study was to test the antibacterial activity of pure pancreatic juice in patients with chronic pancreatitis. METHODS The study group consisted of ten patients with ethanol-induced chronic pancreatitis (AICP) and seven control patients free of pancreatic disease. All subjects had recently undergone a secretin-pancreozymin pancreatic function test. After an overnight fast, through a side-viewing endoscope, selective pancreatic duct cannulation was performed. After secretin stimulation, pure pancreatic juice was obtained. Three fractions of different molecular weights were separated. Samples were incubated with 1-mL suspension of 10(5) Escherichia coli ATCC 25,922, and log10 of colony-forming units were counted. Experiments were repeated by grading pancreatic juice concentration, pH of the medium, and inoculum size. RESULTS No significant change of pH of pure pancreatic juice appeared between AICP and controls. Starting from 6-h observation, pure pancreatic juice of AICP patients showed a significant bacterial colonization vs controls (p < 0.01). A direct correlation appeared between bacterial colonization and either pH and dilution of pancreatic juice (p < 0.001). Antibacterial activity was independent of inoculum size, enzymatic activation or inhibition, and heat treatment. The fraction with 1000-10,000 molecular weight was the one endowed with antibacterial activity.", "gemini": "KẾT LUẬN Các dữ liệu này cho thấy dịch tụy tinh khiết của bệnh nhân AICP có hoạt tính kháng khuẩn bị khiếm khuyết rõ rệt. Phát hiện này có thể có giá trị lâm sàng tiềm tàng trong việc hiểu về bệnh sinh của bệnh. BỐI CẢNH Mục đích của nghiên cứu hiện tại là kiểm tra hoạt tính kháng khuẩn của dịch tụy tinh khiết ở những bệnh nhân bị viêm tụy mãn tính. PHƯƠNG PHÁP Nhóm nghiên cứu bao gồm mười bệnh nhân bị viêm tụy mãn tính do rượu (AICP) và bảy bệnh nhân đối chứng không mắc bệnh tụy. Tất cả các đối tượng gần đây đã trải qua xét nghiệm chức năng tụy secretin-pancreozymin. Sau khi nhịn ăn qua đêm, thông qua nội soi nhìn nghiêng, đã thực hiện đặt ống chọn lọc vào ống tụy. Sau khi kích thích secretin, dịch tụy tinh khiết đã được lấy. Ba phân đoạn có trọng lượng phân tử khác nhau đã được tách ra. Các mẫu được ủ với 1 mL dịch huyền phù 10(5) Escherichia coli ATCC 25.922 và đếm log10 của các đơn vị hình thành khuẩn lạc. Các thí nghiệm được lặp lại bằng cách phân loại nồng độ dịch tụy, độ pH của môi trường và kích thước mẫu cấy. KẾT QUẢ Không có sự thay đổi đáng kể về độ pH của dịch tụy tinh khiết giữa AICP và đối chứng. Bắt đầu từ quan sát 6 giờ, dịch tụy tinh khiết của bệnh nhân AICP cho thấy sự xâm khuẩn của vi khuẩn đáng kể so với đối chứng (p < 0,01). Có mối tương quan trực tiếp giữa sự xâm khuẩn của vi khuẩn và độ pH và độ pha loãng của dịch tụy (p < 0,001). Hoạt tính kháng khuẩn không phụ thuộc vào kích thước mẫu cấy, hoạt hóa hoặc ức chế enzym và xử lý nhiệt. Phân đoạn có trọng lượng phân tử 1000-10.000 là phân đoạn có hoạt tính kháng khuẩn."} +{"idx": 1950096, "en": "CONCLUSIONS These studies show for the first time the localization of a H+/peptide cotrasporter in nuclei of vascular smooth muscle cells and Schwann cells and its localization in lysosomes of the exocrine pancreas. It is likely that the transporter functions to move small peptides from the lysosome to the cytoplasm following intralysosomal protein degradation. The nature of the transporter function in the nucleus remains to be determined, including the possibility that peptide signaling molecules may be transmitted between nucleus and cytoplasm. BACKGROUND PEPT1 transports di- and tripeptides through plasma membranes. Peptides are cotransported with H+, thus deriving the energy for the active transport process from an electrochemical H+ gradient. The main regions in which PEPT1 has been thought to function are the plasma membranes of the small intestinal epithelial cells for absorption of protein digestion products and in the kidney tubules for recovery of small peptides from the glomerular filtrate. METHODS Pancreas was removed from rats and quick frozen with liquid nitrogen. Frozen sections were fixed in cold acetone. Sections were incubated with primary antibody against PEPT1, followed by a secondary antibody conjugated with fluorescein, then examined with a fluorescence microscope. RESULTS Three major structures were immunopositive with the antibody to PEPT1: the nuclei of smooth muscle cells in the wall of arterioles, the nuclei of Schwann cells in unmyelinated pancreatic nerves, and lysosomes in acinar cells.", "gemini": "KẾT LUẬN Các nghiên cứu này lần đầu tiên chỉ ra vị trí của một chất đồng vận chuyển H+/peptide trong nhân của các tế bào cơ trơn mạch máu và tế bào Schwann và vị trí của nó trong lysosome của tuyến tụy ngoại tiết. Có khả năng chất vận chuyển có chức năng di chuyển các peptide nhỏ từ lysosome đến tế bào chất sau quá trình phân hủy protein trong lysosome. Bản chất của chức năng vận chuyển trong nhân vẫn chưa được xác định, bao gồm khả năng các phân tử tín hiệu peptide có thể được truyền giữa nhân và tế bào chất. BỐI CẢNH PEPT1 vận chuyển đi- và tripeptide qua màng plasma. Peptide được vận chuyển đồng thời với H+, do đó lấy năng lượng cho quá trình vận chuyển tích cực từ độ dốc điện hóa H+. Các vùng chính mà người ta cho rằng PEPT1 hoạt động là màng plasma của các tế bào biểu mô ruột non để hấp thụ các sản phẩm tiêu hóa protein và trong ống thận để phục hồi các peptide nhỏ từ dịch lọc cầu thận. PHƯƠNG PHÁP Tuyến tụy được lấy ra khỏi chuột và đông lạnh nhanh bằng nitơ lỏng. Các phần đông lạnh được cố định trong acetone lạnh. Các phần được ủ với kháng thể chính chống lại PEPT1, sau đó là kháng thể thứ cấp được liên hợp với fluorescein, sau đó được kiểm tra bằng kính hiển vi huỳnh quang. KẾT QUẢ Ba cấu trúc chính có miễn dịch dương tính với kháng thể đối với PEPT1: nhân của các tế bào cơ trơn trong thành tiểu động mạch, nhân của các tế bào Schwann trong các dây thần kinh tụy không có myelin và lysosome trong các tế bào tuyến."} +{"idx": 1950097, "en": "The effect of MPP+, a dopaminergic neurotoxin, in mitochondrial membrane potential was investigated in dissociated cerebellar granule cells using rhodamine 123 and flow cytometry. MPP+ (1 mM) decreased the mitochondrial membrane potential by 30%. Antagonists of the NMDA receptor complex, such as MK-801 (IC50 value of 20.92 +/- 0.02 nM), 5,7-dichlorokynurenic acid (IC50 value of 6.46 +/- 1.06 microM) and D-AP5 (IC50 value of 8.29 +/- 0.63 microM), inhibited the action of MPP+. Neither NBQX, nor riluzole, nor desipramine modified the action of MPP+. Dibucaine restored the basal values of mitochondrial membrane potential altered by MPP+. Since, in the presence of NMDA, MPP+ antagonized the effect of this total agonist, it can be concluded that, in this preparation, MPP+ interacts with the NMDA receptor complex as a partial agonist. This interaction could be the result of an allosteric modulation of the NMDA receptor complex by MPP+. The decrease of mitochondrial membrane potential induced by MPP+ is antagonized by dibucaine, suggesting that this effect is mediated by an activation of phospholipase A2.", "gemini": "Tác dụng của MPP+, một chất độc thần kinh dopaminergic, đối với tiềm năng màng ty thể đã được nghiên cứu trong các tế bào hạt tiểu não phân ly bằng cách sử dụng rhodamine 123 và phương pháp đo lưu lượng tế bào. MPP+ (1 mM) làm giảm 30% tiềm năng màng ty thể. Các chất đối kháng của phức hợp thụ thể NMDA, như MK-801 (giá trị IC50 là 20,92 +/- 0,02 nM), axit 5,7-dichlorokynurenic (giá trị IC50 là 6,46 +/- 1,06 microM) và D-AP5 (giá trị IC50 là 8,29 +/- 0,63 microM), ức chế tác dụng của MPP+. Không có NBQX, riluzole hay desipramine làm thay đổi tác dụng của MPP+. Dibucaine phục hồi các giá trị cơ bản của tiềm năng màng ty thể bị MPP+ làm thay đổi. Vì khi có NMDA, MPP+ đối kháng với tác dụng của toàn bộ chất chủ vận này, có thể kết luận rằng trong chế phẩm này, MPP+ tương tác với phức hợp thụ thể NMDA như một chất chủ vận một phần. Tương tác này có thể là kết quả của sự điều biến dị lập thể của phức hợp thụ thể NMDA bởi MPP+. Sự giảm tiềm năng màng ty thể do MPP+ gây ra bị dibucaine đối kháng, cho thấy rằng tác dụng này được trung gian bởi sự hoạt hóa của phospholipase A2."} +{"idx": 1950098, "en": "The effect of enzyme-inhibiting adjuvants on L-DOPA + benserazide-induced contralateral turning in unilateral 6-hydroxydopamine (6-OHDA)-lesioned rats was studied. Both the number of turns and the duration of turning were examined. Inhibition of MAO-A with 10 mg/kg Ro 41-1049 increased both parameters; inhibition of COMT with 30 mg/kg Ro 40-7592 had a similar effect. In contrast, inhibition of MAO-B with 10 mg/kg Ro 19-6327 did not change turning behavior. A further potentiation of turning behavior was observed after the combined administration of both the MAO-A and COMT inhibitor. MAO-A inhibition in conjunction with MAO-B inhibition prolonged the duration of L-DOPA-induced turning but had no effect on the number of turns. However, in conjunction with COMT inhibition, 10 mg/kg of the MAO-B inhibitor, Ro 19-6327, significantly affected both the number and duration of turning behavior. An even further potentiation of turning behavior was observed after the combined administration of all three enzyme-inhibitors.", "gemini": "Nghiên cứu tác dụng của các chất bổ trợ ức chế enzym đối với L-DOPA + benserazide gây quay ngược bên đối diện ở chuột bị tổn thương 6-hydroxydopamine (6-OHDA) một bên. Cả số lần quay và thời gian quay đều được kiểm tra. Ức chế MAO-A với 10 mg/kg Ro 41-1049 làm tăng cả hai thông số; ức chế COMT với 30 mg/kg Ro 40-7592 có tác dụng tương tự. Ngược lại, ức chế MAO-B với 10 mg/kg Ro 19-6327 không làm thay đổi hành vi quay. Một sự thúc đẩy hành vi quay tiếp theo được quan sát thấy sau khi dùng kết hợp cả chất ức chế MAO-A và COMT. Ức chế MAO-A kết hợp với ức chế MAO-B kéo dài thời gian quay do L-DOPA gây ra nhưng không ảnh hưởng đến số lần quay. Tuy nhiên, kết hợp với ức chế COMT, 10 mg/kg chất ức chế MAO-B, Ro 19-6327, ảnh hưởng đáng kể đến cả số lần và thời gian quay. Một sự thúc đẩy hành vi quay thậm chí còn mạnh hơn nữa được quan sát thấy sau khi dùng kết hợp cả ba chất ức chế enzym."} +{"idx": 1950099, "en": "The present experiments were undertaken in order to examine mechanisms of action for reported interactions between the beta-blocker (-)-pindolol and serotonergic agents. It was found that pretreatment with (-)-pindolol (2 mg kg-1 s.c.) potentiated the stereotyped forward locomotion induced by the 5-HT2A/C receptor agonist DOI (0.125-1.0 mg kg-1 s.c.) in rats observed in an open-field arena. This (-)-pindolol/DOI-induced stereotyped forward locomotion was fully antagonized by the 5-HT2A/C receptor antagonist ritanserin (2 mg kg-1 s.c.), suggesting that (-)-pindolol enhances serotonin release, resulting i.a. in postsynaptic 5-HT2A/C receptor activation. This effect by (-)-pindolol is in all probability indirect since this compound lacks affinity for 5-HT2A/C receptors, and could be explained by reported antagonism of inhibitory serotonergic somato-dendritic 5-HT1A autoreceptors, although other possibilities related to 5-HT1B receptors or beta-adrenoceptors can not be excluded at this time. Furthermore, (-)-pindolol treatment also enhanced 5-HTP-induced (12.5-100 mg kg-1 i.p.) effects on spontaneous motor activity. These effects, however, were of smaller magnitude, and less consistent than those seen in combination with DOI.", "gemini": "Các thí nghiệm hiện tại được thực hiện để kiểm tra cơ chế tác động cho các tương tác đã báo cáo giữa thuốc chẹn beta (-)-pindolol và các tác nhân serotonergic. Người ta thấy rằng tiền xử lý bằng (-)-pindolol (2 mg kg-1 s.c.) làm tăng cường chuyển động tiến về phía trước theo kiểu khuôn mẫu do chất chủ vận thụ thể 5-HT2A/C DOI (0,125-1,0 mg kg-1 s.c.) gây ra ở chuột được quan sát thấy trong đấu trường mở. Chuyển động tiến về phía trước theo kiểu khuôn mẫu do (-)-pindolol/DOI gây ra này đã bị đối kháng hoàn toàn bởi chất đối kháng thụ thể 5-HT2A/C ritanserin (2 mg kg-1 s.c.), cho thấy (-)-pindolol làm tăng giải phóng serotonin, dẫn đến hoạt hóa thụ thể 5-HT2A/C sau synap. Tác dụng này của (-)-pindolol rất có thể là gián tiếp vì hợp chất này không có ái lực với thụ thể 5-HT2A/C và có thể được giải thích bằng sự đối kháng đã báo cáo của các thụ thể tự thân 5-HT1A somato-dendrit serotonergic ức chế, mặc dù các khả năng khác liên quan đến thụ thể 5-HT1B hoặc beta-adrenergic không thể loại trừ tại thời điểm này. Hơn nữa, điều trị bằng (-)-pindolol cũng làm tăng cường tác dụng của 5-HTP (12,5-100 mg kg-1 i.p.) đối với hoạt động vận động tự phát. Tuy nhiên, các tác dụng này có cường độ nhỏ hơn và ít nhất quán hơn so với những tác dụng thấy khi kết hợp với DOI."} +{"idx": 1950100, "en": "The study has shown an excitatory influence exerted by lateral habenula (LH) on hippocampal pyramidal cells. The modulatory influence is paradoxically serotonine-mediated; in fact all LH stimulation effects were abolished by intrahippocampal iontophoretic methysergide application. The data suggest the involvement of dorsal raphe nucleus. In fact, the dorsal raphe nucleus stimulation caused on hippocampus an expected inhibitory effect antagonized by intrahippocampal iontophoretic methysergide application. In the context of this neural structure we have highlighted a disinhibitory relation between two types of cells: slow serotonergic efferent neurones and fast GABAergic interneurones. The disinhibitory hypothesis is also supported by the following experimental tests performed on both slow and fast raphe cells: a) LH stimulation at low and high frequencies; b) iontophoretic administration of NMDA and GABA; c) LH stimulation during intraraphe iontophoretic injection of 2-APV (NMDA antagonist) and bicuculline (GABA antagonist).", "gemini": "Nghiên cứu đã chỉ ra ảnh hưởng kích thích do nhân dưới đồi bên (LH) tác động lên các tế bào hình chóp hồi. Ảnh hưởng điều chế này nghịch lý là do serotonin trung gian; trên thực tế, tất cả các tác dụng kích thích LH đều bị xóa bỏ do ứng dụng methysergide iontophoretic trong hồi. Dữ liệu cho thấy sự tham gia của nhân đường giữa lưng. Trên thực tế, kích thích nhân đường giữa lưng gây ra một tác dụng ức chế dự kiến trên hồi bị đối kháng bởi ứng dụng methysergide iontophoretic trong hồi. Trong bối cảnh của cấu trúc thần kinh này, chúng tôi đã làm nổi bật mối quan hệ giải ức chế giữa hai loại tế bào: tế bào thần kinh hướng tâm serotonergic chậm và tế bào thần kinh xen GABAergic nhanh. Giả thuyết giải ức chế cũng được hỗ trợ bởi các xét nghiệm thực nghiệm sau đây được thực hiện trên cả tế bào đường giữa lưng chậm và nhanh: a) Kích thích LH ở tần số thấp và cao; b) dùng iontophoretic NMDA và GABA; c) Kích thích LH trong quá trình tiêm iontophoretic 2-APV (chất đối kháng NMDA) và bicuculline (chất đối kháng GABA) vào trong đường giữa lưng."} +{"idx": 1950101, "en": "The effect of local administration of glutamate into the hypothalamic paraventricular nucleus (PVN) on the hypothalamo-pituitary adrenocortical (HPA) axis was studied in male rats. Glutamate caused CRH-41 depletion from the median eminence (ME) and a consequent rise in ACTH and corticosterone (CS) serum levels. In rats pretreated with systemic dexamethasone (dex) these effects were completely inhibited. The administration of the glucocorticoid receptor antagonist RU-38486 abolished the inhibitory effect of dex on the adrenocortical discharge. In addition, the depletion of hypothalamic norepinephrine (NE) and serotonin (5-HT) by specific neurotoxins administered into the ventral noradrenergic blundle or into the raphe nuclei respectively, inhibited the response of serum ACTH and CS following PVN glutamate administration. These data indicate that glutamate stimulated the HPA axis via the release of ME CRH-41 into the portal circulation. This response is steroid sensitive involving type II glucocorticoid receptors. Hypothalamic NE and 5-HT participate in the glutamate induced HPA axis activation.", "gemini": "Tác dụng của việc dùng glutamate tại chỗ vào nhân quanh thất dưới đồi (PVN) đối với trục hạ đồi-tuyến yên-vỏ thượng thận (HPA) đã được nghiên cứu trên chuột đực. Glutamate gây ra sự suy giảm CRH-41 từ eminentia giữa (ME) và làm tăng nồng độ ACTH và corticosterone (CS) trong huyết thanh. Ở những con chuột được xử lý trước bằng dexamethasone toàn thân (dex), những tác dụng này đã bị ức chế hoàn toàn. Việc dùng thuốc đối kháng thụ thể glucocorticoid RU-38486 đã xóa bỏ tác dụng ức chế của dex đối với sự phóng thích vỏ thượng thận. Ngoài ra, sự suy giảm norepinephrine (NE) và serotonin (5-HT) ở vùng dưới đồi do các chất độc thần kinh đặc hiệu được đưa vào bó sợi noradrenergic bụng hoặc vào nhân đường giữa lưng tương ứng, đã ức chế phản ứng của ACTH và CS trong huyết thanh sau khi dùng glutamate PVN. Các dữ liệu này chỉ ra rằng glutamate kích thích trục HPA thông qua việc giải phóng ME CRH-41 vào tuần hoàn cửa. Phản ứng này nhạy cảm với steroid liên quan đến thụ thể glucocorticoid loại II. NE và 5-HT ở vùng dưới đồi tham gia vào quá trình kích hoạt trục HPA do glutamate gây ra."} +{"idx": 1950102, "en": "The present study investigated the effect of dim white light on nocturnal plasma melatonin in males and females. Subjects were exposed to light between 2400 hr and 0100 hr. No significant gender differences were found with both 200 lux (p > 0.1) and 500 lux (p > 0.1) of light. Furthermore the amplitude of the melatonin rhythm was not significantly different with gender. This suggests that at low intensities the melatonin sensitivity to light is not differentially regulated between sexes.", "gemini": "Nghiên cứu hiện tại đã điều tra tác động của ánh sáng trắng mờ lên melatonin huyết tương vào ban đêm ở nam và nữ. Những người tham gia tiếp xúc với ánh sáng từ 2400 giờ đến 0100 giờ. Không có sự khác biệt đáng kể nào về giới tính được tìm thấy với cả 200 lux (p> 0,1) và 500 lux (p> 0,1) ánh sáng. Hơn nữa, biên độ của nhịp melatonin không khác biệt đáng kể về giới tính. Điều này cho thấy ở cường độ thấp, độ nhạy của melatonin đối với ánh sáng không được điều chỉnh khác nhau giữa các giới tính."} +{"idx": 1950103, "en": "In this study a chronic cerebral iron-loaded model was established by feeding mice with high iron diet. Data indicated that brain iron concentrations were significantly increased in iron-fed mice compared with those of controls. A significant increase in oxidized glutathione (GSSG), decrease in total glutathione (oxidized and reduced glutathione, GSSG + GSH), and therefore increase in the GSSG/(GSSG + GSH) ratios were observed in iron-loaded mice. Hydroxyl radical (.OH) levels in striatum and brainstem were also significantly increased. Excessive iron alone did not change either dopamine (DA) or lipid peroxidation (LPO) concentrations in striatum. However, a single injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg, i.p.) into the iron-loaded mice caused a great enhancement in all these biochemical abnormalities. These findings suggest that iron does induce oxidative stress, but not severely injury neurons per sc. Excessive iron accumulation in the brain, however, is a potential risk for neuronal damage, which may promote by triggering factor(s). This supports the hypothesis that excessive cerebral iron may contribute to the aetiology of Parkinson's disease (PD).", "gemini": "Trong nghiên cứu này, một mô hình động vật nạp sắt mạn tính trong não đã được thiết lập bằng cách cho chuột ăn chế độ ăn nhiều sắt. Dữ liệu chỉ ra rằng nồng độ sắt trong não tăng đáng kể ở những con chuột được cho ăn sắt so với nhóm đối chứng. Sự gia tăng đáng kể glutathione bị oxy hóa (GSSG), giảm glutathione toàn phần (glutathione bị oxy hóa và khử, GSSG + GSH) và do đó làm tăng tỷ lệ GSSG/(GSSG + GSH) được quan sát thấy ở những con chuột được nạp sắt. Nồng độ gốc hydroxyl (.OH) ở thể vân và thân não cũng tăng đáng kể. Chỉ riêng sắt dư thừa không làm thay đổi nồng độ dopamine (DA) hoặc lipid peroxy hóa (LPO) ở thể vân. Tuy nhiên, một lần tiêm 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg, i.p.) vào những con chuột được nạp sắt đã gây ra sự gia tăng đáng kể trong tất cả những bất thường về sinh hóa này. Những phát hiện này cho thấy sắt thực sự gây ra stress oxy hóa, nhưng không làm tổn thương nghiêm trọng các tế bào thần kinh. Tuy nhiên, sự tích tụ sắt quá mức trong não là một nguy cơ tiềm ẩn đối với tổn thương tế bào thần kinh, có thể thúc đẩy các yếu tố kích hoạt. Điều này hỗ trợ cho giả thuyết rằng sắt não dư thừa có thể góp phần vào nguyên nhân gây bệnh Parkinson (PD)."} +{"idx": 1950104, "en": "Depletion of reduced glutathione occurs in the substantia nigra in Parkinson's disease and in incidental Lewy body disease (presymptomatic Parkinson's disease) which may implicate oxidative stress in the neurodegenerative process. In this study mercury orange fluorescent staining and immunostaining with an antibody to reduced glutathione have been used to determine the distribution of reduced glutathione in the substantia nigra in Parkinson's disease compared with normal individuals. Mercury orange staining showed moderate background levels of fluorescence in the neuropil in both control and Parkinson's disease substantia nigra and localised reduced glutathione to the somata of melanized nigral neurons and glial elements of the neuropil. Neuronal nuclei revealed a relative lack of fluorescence after mercury orange staining. There was a significant depletion of reduced glutathione in surviving neurons in Parkinson's disease compared to nerve cell populations in control tissue. Mercury orange fluorescence indicated a high concentration of reduced glutathione in a subpopulation of non-neuronal cells, most likely astrocytes or microglia. Immunohistochemical examination of nigral tissue from the same Parkinson's disease and control patients with an antibody to glutathione showed staining in neuronal perikarya and axonodendritic processes of melanized nigral neurons which was generally most intense in control neurons. Moderately intense staining of the background neuropil, most prominent in control nigras, and staining of capillary walls was also detected. Intense staining was seen in cells with the morphological features of glial cells in both control and PD nigra. These data show a significant presence of reduced glutathione in the cell bodies and axons of nigral neurons. They are in agreement with biochemical studies showing depletion of reduced glutathione in substantia nigra in Parkinson's disease, and indicate a significant loss of neuronal reduced glutathione in surviving nigral neurons in Parkinson's disease.", "gemini": "Sự suy giảm glutathione khử xảy ra ở chất đen trong bệnh Parkinson và trong bệnh thể Lewy ngẫu nhiên (bệnh Parkinson trước triệu chứng) có thể liên quan đến stress oxy hóa trong quá trình thoái hóa thần kinh. Trong nghiên cứu này, nhuộm huỳnh quang màu cam thủy ngân và nhuộm miễn dịch bằng kháng thể với glutathione khử đã được sử dụng để xác định sự phân bố của glutathione khử trong chất đen ở bệnh Parkinson so với những người bình thường. Nhuộm màu cam thủy ngân cho thấy mức độ huỳnh quang nền vừa phải ở sợi thần kinh đệm ở cả chất đen đối chứng và chất đen của bệnh Parkinson và glutathione khử cục bộ ở thân tế bào của các tế bào thần kinh đen bị thoái hóa và các thành phần thần kinh đệm của sợi thần kinh đệm. Nhân tế bào thần kinh cho thấy sự thiếu huỳnh quang tương đối sau khi nhuộm màu cam thủy ngân. Có sự suy giảm đáng kể glutathione khử ở các tế bào thần kinh còn sống trong bệnh Parkinson so với quần thể tế bào thần kinh trong mô đối chứng. Huỳnh quang màu cam thủy ngân chỉ ra nồng độ glutathione khử cao ở một nhóm tế bào không phải tế bào thần kinh, rất có thể là tế bào hình sao hoặc tế bào microglia. Kiểm tra miễn dịch mô học về mô đen từ cùng một bệnh nhân mắc bệnh Parkinson và đối chứng bằng kháng thể với glutathione cho thấy nhuộm màu ở chu vi nhân tế bào thần kinh và các nhánh sợi trục của các tế bào thần kinh đen bị thoái hóa, thường mạnh nhất ở các tế bào thần kinh đối chứng. Nhuộm màu vừa phải của sợi thần kinh đệm nền, nổi bật nhất ở chất đen đối chứng và nhuộm màu thành mạch mao mạch cũng được phát hiện. Nhuộm màu mạnh được nhìn thấy ở các tế bào có đặc điểm hình thái của tế bào thần kinh đệm ở cả chất đen đối chứng và PD. Những dữ liệu này cho thấy sự hiện diện đáng kể của glutathione khử trong thân tế bào và sợi trục của các tế bào thần kinh đen. Chúng phù hợp với các nghiên cứu sinh hóa cho thấy sự suy giảm glutathione khử ở chất đen trong bệnh Parkinson và chỉ ra sự mất glutathione khử ở tế bào thần kinh đáng kể ở các tế bào thần kinh đen còn sống trong bệnh Parkinson."} +{"idx": 1950105, "en": "Recovery curves of the R2 component of the blink reflex have been studied in 10 control subjects and 13 parkinsonian patients both after ipsilateral paired stimulation of the supraorbital nerve and after index finger stimulation. In control subjects, both types of conditioning induced a comparable marked inhibition lasting more than 600 ms. In parkinsonian patients, inhibition was reduced after both conditionings. However, differences appeared in the magnitude of the changes: after paired stimulation, it was less significant (ANOVA and post-hoc Duncan's test: p = 0.04) than after index finger stimulation (p = 0.002). In that latter situation, the more marked reduction in inhibition is interpreted, in the light of current physiologic knowledge, by hypoactivity of the Nucleus Reticularis Giganto Cellularis (NRGC) which would make less efficient inhibitory interneurones in the trigemino-facial pathway. The results are thus compatible with the suggestion that NRGC is made indirectly less active in Parkinson's disease.", "gemini": "Đường cong phục hồi của thành phần R2 phản xạ chớp mắt đã được nghiên cứu ở 10 đối tượng chứng và 13 bệnh nhân parkinson sau khi kích thích ghép đôi cùng bên dây thần kinh trên ổ mắt và sau khi kích thích ngón trỏ. Ở đối tượng chứng, cả hai loại điều kiện đều gây ra sự ức chế đáng kể tương đương kéo dài hơn 600 ms. Ở bệnh nhân parkinson, sự ức chế giảm sau cả hai điều kiện. Tuy nhiên, sự khác biệt xuất hiện trong biên độ của những thay đổi: sau khi kích thích ghép đôi, nó ít có ý nghĩa hơn (ANOVA và kiểm định Duncan sau thử nghiệm: p = 0,04) so với sau khi kích thích ngón trỏ (p = 0,002). Trong tình huống sau đó, sự giảm ức chế rõ rệt hơn được giải thích, theo kiến thức sinh lý hiện tại, là do hoạt động kém của nhân lưới khổng lồ (NRGC) khiến các tế bào thần kinh ức chế ít hiệu quả hơn trong đường dẫn tam thoa-mặt. Do đó, kết quả tương thích với gợi ý rằng NRGC hoạt động kém đi gián tiếp trong bệnh Parkinson."} +{"idx": 1950106, "en": "The purpose of this study was to analyse the frequency of the different pathological perfusion patterns in SPECT in a clinical, unselected population of patients with Alzheimer's disease. In 91 patients and 16 control subjects regional cerebral blood flow (rCBF) was measured with Single Photon Emission Computed Tomography (SPECT) using 99mTc-hexa-methyl-propyleneamine oxime (HMPAO). 95% confidence intervals obtained from the perfusion values of the control subjects were used to define normal perfusion ranges. The frequency of perfusion deficits in the left frontal, temporal, parietal and occipital lobes were 62.2%, 60.4%. 70.3% and 23.1%, respectively. In the right hemisphere the corresponding values were 60.4%, 58.2%, 63.7% and 9.9%. With the exception of the occipital lobes these frequencies were not significantly different. The analysis of the perfusion pattern of each patient revealed 35 different combinations of lobes with perfusion deficits. The temporo-parietal perfusion deficits were not more frequent than the temporofrontal perfusion deficits. These results suggest that in the clinical routine a high variety of heterogeneous rCBF patterns have to be expected.", "gemini": "Mục đích của nghiên cứu này là phân tích tần suất của các kiểu tưới máu bệnh lý khác nhau trong SPECT ở một quần thể bệnh nhân mắc bệnh Alzheimer không được lựa chọn trên lâm sàng. Ở 91 bệnh nhân và 16 đối tượng chứng, lưu lượng máu não khu vực (rCBF) được đo bằng Chụp cắt lớp phát xạ một photon (SPECT) sử dụng 99mTc-hexa-methyl-propyleneamine oxime (HMPAO). Các khoảng tin cậy 95% thu được từ các giá trị tưới máu của đối tượng chứng được sử dụng để xác định phạm vi tưới máu bình thường. Tần suất thiếu hụt tưới máu ở thùy trán trái, thái dương, đỉnh và chẩm lần lượt là 62,2%, 60,4%. 70,3% và 23,1%. Ở bán cầu phải, các giá trị tương ứng là 60,4%, 58,2%, 63,7% và 9,9%. Ngoại trừ thùy chẩm, các tần suất này không khác biệt đáng kể. Phân tích kiểu tưới máu của từng bệnh nhân cho thấy 35 tổ hợp khác nhau của các thùy có thiếu hụt tưới máu. Thiếu hụt tưới máu thái dương-đỉnh không thường xuyên hơn thiếu hụt tưới máu thái dương-trán. Những kết quả này cho thấy rằng trong thực hành lâm sàng, người ta phải mong đợi có nhiều kiểu rCBF không đồng nhất."} +{"idx": 1950107, "en": "We compared CSF and serum levels, and the CST/serum ratio of alpha-tocopherol (vitamin E), measured by HPLC, in 44 apparently well-nourished patients with Alzheimer's disease (AD) and 37 matched controls. CSF and serum vitamin E levels were correlated, both in AD patients and in controls. The mean CSF and serum vitamin E levels were significantly lower in AD patients, and the CSF/serum ratio of AD patients did not differ significantly between the 2 study groups. CSF vitamin E levels did not correlate with age, age at onset, duration of the disease and score of the Minimental State Examination in the AD group. Weight and body mass index were significantly lower in AD patients than in controls. These results suggest that low CSF and serum vitamin E concentrations in AD patients could be related with a deficiency of dietary intake of vitamin E.", "gemini": "Chúng tôi đã so sánh nồng độ CSF và huyết thanh, và tỷ lệ CST/huyết thanh của alpha-tocopherol (vitamin E), được đo bằng HPLC, ở 44 bệnh nhân được chẩn đoán là mắc bệnh Alzheimer (AD) và 37 đối chứng phù hợp. Nồng độ vitamin E trong CSF và huyết thanh có tương quan, ở cả bệnh nhân AD và đối chứng. Nồng độ vitamin E trung bình trong CSF và huyết thanh thấp hơn đáng kể ở bệnh nhân AD và tỷ lệ CSF/huyết thanh của bệnh nhân AD không khác biệt đáng kể giữa 2 nhóm nghiên cứu. Nồng độ vitamin E trong CSF không tương quan với tuổi, tuổi khởi phát, thời gian mắc bệnh và điểm của Bài kiểm tra trạng thái tinh thần ở nhóm AD. Cân nặng và chỉ số khối cơ thể thấp hơn đáng kể ở bệnh nhân AD so với đối chứng. Những kết quả này cho thấy rằng nồng độ vitamin E trong CSF và huyết thanh thấp ở bệnh nhân AD có thể liên quan đến tình trạng thiếu hụt vitamin E trong chế độ ăn uống."} +{"idx": 1950108, "en": "We have developed a new procedure, including three affinity chromatography steps, micro-reversed phase high pressure liquid chromatography (mR-HPLC) and Western blotting/mass spectrometric analysis to study central nervous system (CNS) specific proteins in human cerebro spinal fluid (CSF) in order to find biochemical markers for neuronal and synaptic function and pathology in degenerative brain disorders. After the three affinity chromatography steps, intended to remove interfering serum proteins from CSF, mR-HPLC revealed four major peaks, which by both Western blotting and mass spectrometric analyses were found to correspond to beta 2-microglobulin, cystatin C, transthyretin (TTR) and asialotransferrin. When comparing these peaks in CSF from Alzheimer's disease (AD) patients and age-matched healthy controls, a reduction of the brain-specific TTR was found. Therefore we quantified TTR in CSF and serum samples from 8 patients with early onset AD (EAD), 18 patients with late onset AD (LAD), 8 patients with vascular dementia (VAD) and 18 healthy individuals using a nephelometric method. CSF-TTR was divided into barrier-dependent and barrier-independent TTR. The barrier-independent i.e. brain-specific TTR was significantly reduced in the EAD group compared to the controls. Transthyretin has been found to be present in the senile plaques in AD, and to specifically bind to beta/A4 protein, the major component of the amyloid deposits in AD. Therefore, the reduction of the transthyretin-isoform in CSF in AD may reflect an absorption of transthyretin to the amyloid deposits in the senile plaques.", "gemini": "Chúng tôi đã phát triển một quy trình mới, bao gồm ba bước sắc ký ái lực, sắc ký lỏng hiệu suất cao pha đảo ngược vi mô (mR-HPLC) và phân tích khối phổ/chuyển điện phương Tây để nghiên cứu các protein đặc hiệu của hệ thần kinh trung ương (CNS) trong dịch não tủy (CSF) ở người nhằm tìm ra các dấu hiệu sinh hóa cho chức năng và bệnh lý học của nơ-ron và synap trong các rối loạn thoái hóa não. Sau ba bước sắc ký ái lực, nhằm loại bỏ các protein huyết thanh gây nhiễu khỏi CSF, mR-HPLC cho thấy bốn đỉnh chính, mà cả phân tích chuyển điện phương Tây và khối phổ đều cho thấy tương ứng với beta 2-microglobulin, cystatin C, transthyretin (TTR) và asialotransferrin. Khi so sánh các đỉnh này trong CSF từ bệnh nhân mắc bệnh Alzheimer (AD) và những người đối chứng khỏe mạnh cùng độ tuổi, người ta thấy có sự giảm TTR đặc hiệu của não. Do đó, chúng tôi định lượng TTR trong CSF và các mẫu huyết thanh từ 8 bệnh nhân mắc AD khởi phát sớm (EAD), 18 bệnh nhân mắc AD khởi phát muộn (LAD), 8 bệnh nhân mắc chứng mất trí do mạch máu (VAD) và 18 cá nhân khỏe mạnh bằng phương pháp đo độ đục. CSF-TTR được chia thành TTR phụ thuộc vào hàng rào và TTR không phụ thuộc vào hàng rào. TTR không phụ thuộc vào hàng rào tức là TTR đặc hiệu của não giảm đáng kể ở nhóm EAD so với nhóm đối chứng. Người ta thấy Transthyretin có mặt trong các mảng bám già ở AD và liên kết đặc hiệu với protein beta/A4, thành phần chính của các lắng đọng amyloid ở AD. Do đó, sự giảm của dạng đồng phân transthyretin trong CSF ở AD có thể phản ánh sự hấp thụ transthyretin vào các lắng đọng amyloid trong các mảng bám già."} +{"idx": 1950109, "en": "Immunoreactivities of four subtypes of adenylyl cyclase (AC) (types I, II, IV and V/VI), and basal, forskolin- and Mn(2+)-stimulated AC activities with or without calcium and calmodulin (Ca2+/CaM) were estimated in parietal cortex membranes from cases with dementia of the Alzheimer type (DAT) and age-matched controls. Immunoreactivities of AC-I and AC-II were significantly decreased, but those of AC-IV and AC-V/VI did not change in DAT brains. There was a significant correlation of AC-I immunoreactivity with Ca2+/CaM-sensitive AC activity, but not with the Ca2+/CaM-insensitive activity. Ca2+/CaM-sensitive AC activity was significantly lower in DAT than in the control, indicating that impairment of Ca2+/CaM-sensitive AC-I is clearly involved in the pathophysiology of DAT.", "gemini": "Các phản ứng miễn dịch của bốn phân nhóm của adenylyl cyclase (AC) (các loại I, II, IV và V/VI) và các hoạt động AC cơ bản, được kích thích bởi forskolin và Mn(2+) có hoặc không có canxi và calmodulin (Ca2+/CaM) đã được ước tính trong màng vỏ não đỉnh từ các trường hợp mắc chứng mất trí loại Alzheimer (DAT) và những người đối chứng cùng độ tuổi. Các phản ứng miễn dịch của AC-I và AC-II giảm đáng kể, nhưng các phản ứng miễn dịch của AC-IV và AC-V/VI không thay đổi trong não DAT. Có một mối tương quan đáng kể giữa phản ứng miễn dịch AC-I với hoạt động AC nhạy cảm với Ca2+/CaM, nhưng không có mối tương quan với hoạt động không nhạy cảm với Ca2+/CaM. Hoạt động AC nhạy cảm với Ca2+/CaM thấp hơn đáng kể ở DAT so với nhóm đối chứng, cho thấy rằng sự suy giảm của AC-I nhạy cảm với Ca2+/CaM rõ ràng có liên quan đến bệnh sinh của DAT."} +{"idx": 1950110, "en": "In the present study, the actions of several compounds with different intrinsic activities and BDZ receptor selectivity were examined in two well established animal models of anxiety: the open field test (OFT) and Vogel's punished drinking text (VT). Full agonists at the BDZ GABAA receptor (midazolam and diazepam) showed anxiolytic-like effects in both tests; however, the doses necessary to disinhibit animal behavior controlled by fear were higher in the VT than in the OFT. None of the partial BDZ receptor agonists studied (bretazenil, Ro 19-8022 and abecarnil) diminished neophobia-like behavior of rats in the OFT, and their sedative influence on gross behavior prevailed. On the other hand, all three drugs produced a clear-cut anxiolytic effect in the VT. A selective BDZ, receptor subtype full agonist (zolpidem) had a similar profile of action to that of partial agonists with an even stronger sedative effect in the OFT. Alpidem (a selective BDZ1 receptor partial agonist) did not reveal any anxiolytic action in either test. Flumazenil (an antagonist at the BDZ-GABAA receptors) also produced no effect in the OFT, or the VT. An inverse BDZ receptor agonist, beta-carboline-3-carboxylate methyl ester (beta-CCM), evoked an anxiogenic-like response in the OFT, but not in the VT. In summary, it appeared that partial agonists and selective ligands at BDZ1 receptors revealed less advantageous anxiolytic-like action than did full allosteric GABAA receptor modulators. This study also indicates the test dependent profiles of action of BDZ-GABAA receptor ligands. It also indirectly suggests a different neurobiological background underlying the applied tests.", "gemini": "Trong nghiên cứu hiện tại, các tác động của một số hợp chất có các hoạt động nội tại và độ chọn lọc thụ thể BDZ khác nhau đã được kiểm tra trong hai mô hình động vật lo âu được thiết lập tốt: thử nghiệm vùng mở (OFT) và văn bản uống bị phạt của Vogel (VT). Các chất chủ vận đầy đủ tại thụ thể BDZ GABAA (midazolam và diazepam) cho thấy tác dụng giống như thuốc giải lo âu ở cả hai thử nghiệm; tuy nhiên, liều cần thiết để giải ức chế hành vi của động vật do sợ hãi cao hơn ở VT so với OFT. Không có chất chủ vận một phần nào của thụ thể BDZ được nghiên cứu (bretazenil, Ro 19-8022 và abecarnil) làm giảm hành vi sợ cái mới lạ của chuột trong OFT và ảnh hưởng gây an thần của chúng đối với hành vi tổng thể vẫn chiếm ưu thế. Mặt khác, cả ba loại thuốc đều tạo ra tác dụng giải lo âu rõ ràng trong VT. Một chất chủ vận đầy đủ của phân nhóm thụ thể BDZ, chọn lọc (zolpidem) có cấu hình tác dụng tương tự như các chất chủ vận một phần với tác dụng an thần thậm chí mạnh hơn ở OFT. Alpidem (một chất chủ vận một phần của thụ thể BDZ1 chọn lọc) không cho thấy bất kỳ tác dụng giải lo âu nào trong cả hai thử nghiệm. Flumazenil (một chất đối kháng tại các thụ thể BDZ-GABAA) cũng không tạo ra tác dụng nào trong OFT hoặc VT. Một chất chủ vận ngược của thụ thể BDZ, beta-carboline-3-carboxylate methyl este (beta-CCM), gây ra phản ứng giống như gây lo âu ở OFT, nhưng không ở VT. Tóm lại, có vẻ như các chất chủ vận một phần và các phối tử chọn lọc tại các thụ thể BDZ1 cho thấy tác dụng giống như giải lo âu ít có lợi hơn so với các chất điều biến thụ thể GABAA dị lập thể đầy đủ. Nghiên cứu này cũng chỉ ra các cấu hình tác dụng phụ thuộc vào thử nghiệm của các phối tử thụ thể BDZ-GABAA. Nó cũng gián tiếp gợi ý một nền tảng thần kinh sinh học khác nhau làm cơ sở cho các thử nghiệm được áp dụng."} +{"idx": 1950111, "en": "The aim of this study was to examine the effects of subchronic administration of psychoactive drugs on the alpha subunits of G proteins in the rat brain, and also to determine if different classes of psychoactive drugs share a common property, i.e., of altering levels of these proteins. For this purpose, we selected the psychoactive drugs desipramine and phenelzine (antidepressants), lithium (antimanic), alprazolam and buspirone (anxiolytics), and metachlorophenylpiperazine (anxiogenic). The levels of alpha subunits of G proteins (Gs, Gi 1/2, Gq/11) expressed in cortical, hippocampal, and cerebellar brain regions were studied by the Western blot technique. We observed that subchronic treatment with lithium significantly decreased, and with phenelzine significantly increased levels of Gi 1/2 alpha protein in the cortex and the hippocampus. On the other hand, buspirone significantly decreased levels of Gi 1/2 alpha protein only in the cerebellum. Other psychoactive drugs, however, namely desipramine, meta-chlorophenylpiperazine, and alprazolam, did not alter levels of Gs, Gi 1/2, or Gq/11 alpha proteins in any of the brain regions studied. Since other studies have shown the involvement of G proteins in the mechanism of action of psychoactive drugs, our results demonstrate that expressed protein levels of the alpha subunit of G proteins are not altered by all the psychoactive drugs.", "gemini": "Mục đích của nghiên cứu này là kiểm tra tác dụng của việc dùng thuốc hướng thần dưới mạn tính đối với các tiểu đơn vị alpha của protein G trong não chuột, và cũng để xác định xem các loại thuốc hướng thần khác nhau có cùng một đặc tính hay không, tức là làm thay đổi nồng độ của các protein này. Với mục đích này, chúng tôi đã chọn các loại thuốc hướng thần desipramine và phenelzine (thuốc chống trầm cảm), lithium (thuốc chống hưng cảm), alprazolam và buspirone (thuốc an thần) và metachlorophenylpiperazine (thuốc gây lo âu). Nồng độ các tiểu đơn vị alpha của protein G (Gs, Gi 1/2, Gq/11) được biểu hiện ở các vùng não vỏ, hồi hải mã và tiểu não được nghiên cứu bằng kỹ thuật Western blot. Chúng tôi quan sát thấy rằng điều trị dưới mạn tính bằng lithium làm giảm đáng kể, và điều trị bằng phenelzine làm tăng đáng kể nồng độ protein alpha Gi 1/2 ở vỏ não và hồi hải mã. Mặt khác, buspirone làm giảm đáng kể nồng độ protein alpha Gi 1/2 chỉ ở tiểu não. Tuy nhiên, các loại thuốc hướng thần khác, cụ thể là desipramine, meta-chlorophenylpiperazine và alprazolam, không làm thay đổi nồng độ protein alpha Gs, Gi 1/2 hoặc Gq/11 ở bất kỳ vùng não nào được nghiên cứu. Vì các nghiên cứu khác đã chỉ ra sự tham gia của protein G vào cơ chế hoạt động của thuốc hướng thần, nên kết quả của chúng tôi chứng minh rằng nồng độ protein biểu hiện của tiểu đơn vị alpha của protein G không bị thay đổi bởi tất cả các loại thuốc hướng thần."} +{"idx": 1950112, "en": "To examine whether serum excitatory amino acid concentrations change with clozapine treatment and whether these changes correlate with improvement in negative symptoms, serum excitatory amino acids were measured and clinical scales administered in seven subjects with schizophrenia before and after switching from conventional neuroleptics to clozapine. Clozapine treatment was associated with increased serum glutamate and aspartate concentrations. Clinical improvement was negatively correlated with baseline glycine concentrations. These results support the hypothesis that clozapine acts at least in part by increasing glutamatergic activity.", "gemini": "Để kiểm tra xem nồng độ axit amin kích thích trong huyết thanh có thay đổi khi điều trị bằng clozapine hay không và liệu những thay đổi này có tương quan với sự cải thiện các triệu chứng tiêu cực hay không, nồng độ axit amin kích thích trong huyết thanh đã được đo và các thang đánh giá lâm sàng được thực hiện ở bảy đối tượng mắc bệnh tâm thần phân liệt trước và sau khi chuyển từ thuốc an thần kinh thông thường sang clozapine. Điều trị bằng clozapine có liên quan đến việc tăng nồng độ glutamate và aspartate trong huyết thanh. Sự cải thiện lâm sàng có tương quan âm với nồng độ glycine ban đầu. Những kết quả này ủng hộ giả thuyết cho rằng clozapine hoạt động ít nhất một phần bằng cách tăng hoạt động glutamatergic."} +{"idx": 1950113, "en": "A promising strategy in the treatment of malignant gliomas involves the creation of Herpes Simplex Virus-thymidine kinase (HSV-tk) modified tumor cells. Some authors have observed complete tumor regression after ganciclovir (GCV) treatment of established gliomas transduced in vivo by the HSV-tk gene. Yet, further investigations did not confirm completely these results, even if confirmed the therapeutic potential of such a therapy. Using the rat C6 glioblastoma as a model of malignant brain tumor, we investigated the efficacy of in vivo and in vitro transduction of growing brain tumors with the HSV-tk gene, followed by GCV administration. The stereotactic injection into the left striatum of C6 cells mixed with retroviral producer cells and GCV treatment did not improve significantly the animal survival compared to controls. On the contrary, there was a significant prolongation of the survival of rats inoculated with C6 cells engineered in vitro to express the HSV-tk gene. Nevertheless, complete eradication of the tumors was not achieved. We also injected a group of six rats with a mixture of cells expressing the murine Interleukin-4 (IL-4) gene and C6 cells. IL-4 has been shown to produce the regression of experimental brain tumors. Our preliminary experience seems to confirm that hypothesis. Our results indicate that the outcome of HSV-tk gene therapy can be limited not only by low gene transfer but also by insufficient delivery of GCV to tumor cells. Combined strategies, based on contemporary transduction of HSV-tk and IL-4, may enhance the therapeutic perspectives of such a therapy.", "gemini": "Một chiến lược đầy hứa hẹn trong điều trị u thần kinh đệm ác tính liên quan đến việc tạo ra các tế bào khối u được biến đổi bằng virus Herpes Simplex-thymidine kinase (HSV-tk). Một số tác giả đã quan sát thấy khối u thoái triển hoàn toàn sau khi điều trị bằng ganciclovir (GCV) đối với các khối u thần kinh đệm đã được thiết lập được chuyển đổi trong cơ thể sống bằng gen HSV-tk. Tuy nhiên, các nghiên cứu sâu hơn đã không xác nhận hoàn toàn những kết quả này, ngay cả khi xác nhận tiềm năng điều trị của liệu pháp như vậy. Sử dụng u nguyên bào thần kinh đệm C6 ở chuột làm mô hình khối u não ác tính, chúng tôi đã nghiên cứu hiệu quả của việc chuyển đổi trong cơ thể sống và trong ống nghiệm các khối u não đang phát triển bằng gen HSV-tk, sau đó là dùng GCV. Tiêm định vị vào thể vân trái các tế bào C6 trộn với các tế bào sản xuất retrovirus và điều trị bằng GCV không cải thiện đáng kể tỷ lệ sống sót của động vật so với nhóm đối chứng. Ngược lại, có sự kéo dài đáng kể thời gian sống sót của những con chuột được tiêm các tế bào C6 được thiết kế trong ống nghiệm để biểu hiện gen HSV-tk. Tuy nhiên, khối u không được loại bỏ hoàn toàn. Chúng tôi cũng tiêm cho một nhóm sáu con chuột một hỗn hợp các tế bào biểu hiện gen Interleukin-4 (IL-4) của chuột và các tế bào C6. IL-4 đã được chứng minh là có tác dụng làm thoái triển các khối u não thực nghiệm. Kinh nghiệm ban đầu của chúng tôi dường như xác nhận giả thuyết đó. Kết quả của chúng tôi chỉ ra rằng kết quả của liệu pháp gen HSV-tk có thể bị hạn chế không chỉ do chuyển gen thấp mà còn do việc cung cấp GCV không đủ cho các tế bào khối u. Các chiến lược kết hợp, dựa trên việc chuyển đổi đồng thời HSV-tk và IL-4, có thể nâng cao triển vọng điều trị của liệu pháp như vậy."} +{"idx": 1950114, "en": "UNLABELLED RESEARCH OBJECT: This study intends to consider an encephalocele experimental model, obtained in embryonate eggs, treated in a post-neurulation phase with chemical teratogens. This study intends to point out possibility that pathogenic process, which have determined a malformation, is referable to an original defect of embryonic cranial coatings development and that neurulation defect is secondary. EXPERIMENTAL PLAN Chick and duck embryonate eggs have been used. They have been inoculated in their development phase with known chemical teratogens, as Dintoina and Blue Trypan. A controlling group has been inoculated with physiological solution and, then, it has been followed till the hatching. The experimental group has been undergone to artificial hatching, according to prearranged conditions and it has been analysed during different phases of its development. MEASURES Four cranioencephalic malformations have been obtained: three cases of encephalocele and one case of exencephalia. The individual pathological compounds have been studied under the morphological and histopathological profile. The skull base dimensions have been taken and then compared with the controlling group ones. CONCLUSIONS In the light of experimental data, some considerations have been undertaken considering the pathogenetic hypothesis findable in medical literature, stressing the possibility that encephalocele may be arranged as a post-neurulation defect and that, for taxonomic aims, it has to fit in a different group within cranial disraphims.", "gemini": "ĐỐI TƯỢNG NGHIÊN CỨU KHÔNG ĐƯỢC GÁN NHÃN: Nghiên cứu này dự định xem xét một mô hình thực nghiệm não úng thủy, thu được ở trứng phôi, được xử lý trong giai đoạn sau khi tạo nơron bằng các chất gây quái thai hóa học. Nghiên cứu này dự định chỉ ra khả năng quá trình gây bệnh, đã xác định một dị tật, có thể quy cho một khiếm khuyết ban đầu trong quá trình phát triển lớp phủ hộp sọ của phôi và khiếm khuyết tạo nơron là thứ phát. KẾ HOẠCH THỰC NGHIỆM Đã sử dụng trứng phôi gà và vịt. Chúng đã được tiêm chủng trong giai đoạn phát triển của chúng bằng các chất gây quái thai hóa học đã biết, như Dintoina và Blue Trypan. Một nhóm đối chứng đã được tiêm dung dịch sinh lý, sau đó được theo dõi cho đến khi nở. Nhóm thực nghiệm đã được ấp nhân tạo, theo các điều kiện đã sắp xếp trước và đã được phân tích trong các giai đoạn phát triển khác nhau của nó. CÁC BIỆN PHÁP Đã thu được bốn dị tật sọ não: ba trường hợp não úng thủy và một trường hợp não úng thủy. Các hợp chất bệnh lý riêng lẻ đã được nghiên cứu dưới hồ sơ hình thái và mô bệnh học. Kích thước nền sọ đã được lấy và sau đó so sánh với kích thước của nhóm đối chứng. KẾT LUẬN Theo dữ liệu thực nghiệm, một số cân nhắc đã được thực hiện xem xét giả thuyết về sinh bệnh học có thể tìm thấy trong tài liệu y khoa, nhấn mạnh khả năng não úng thủy có thể được sắp xếp như một khiếm khuyết sau khi tạo nơron và đối với mục đích phân loại, nó phải phù hợp với một nhóm khác nhau trong các dị tật sọ."} +{"idx": 1950115, "en": "BACKGROUND In this experimental study is illustrated an original model of cerebral asymmetric ischemia and reperfusion in the rat, induced by unilaterally elevating ICP and clamping the corresponding common carotid artery, that allows a direct comparison of the two brain hemispheres, one normal and the other ischemic, of the same animal. METHODS The experimental procedure consisted in grafting two screws through the skull on the right side of the sagittal suture, one of them being connected to a Queckenstedt manometer for monitoring ICP variations. A nitroprusside solution (1 mg/ml administered through the femoral vein at a flow rate of 0.103 ml/min) was infused to achieve a significant drop of MABP. At this time point, animals were subjected to 5 min of ischemia and 10 min of reperfusion induced by clamping and declamping the right common carotid artery. During the whole period of ischemia and reperfusion ICP and MABP were constantly monitored. In order to provide an outlook on the metabolic alterations of brain tissue occurring during ischemia and reperfusion phenomena, several biochemical parameters of cellular energy metabolism and of oxygen radical-induced membrane damage were determined by a sensitive and reproducible HPLC method on perchloric acid tissue extracts. RESULTS AND CONCLUSIONS The validity of the present model was supported by the finding of significant intrahemispheric differences in the concentration of several compounds considered as biochemical markers of tissue injury, such as adenosine 5'-triphosphate catabolites and malondialdehyde, this last indicating the damaging action of oxygen free radicals on cell membrane phospholipids.", "gemini": "BỐI CẢNH Trong nghiên cứu thực nghiệm này, mô tả một mô hình ban đầu về thiếu máu cục bộ và tái tưới máu não không đối xứng ở chuột, gây ra bằng cách nâng ICP đơn phương và kẹp động mạch cảnh chung tương ứng, cho phép so sánh trực tiếp hai bán cầu não, một bình thường và một thiếu máu cục bộ, của cùng một động vật. PHƯƠNG PHÁP Quy trình thực nghiệm bao gồm ghép hai vít xuyên hộp sọ ở bên phải của đường nối giữa xương đỉnh, một trong số chúng được kết nối với đồng hồ đo Queckenstedt để theo dõi các biến thể ICP. Dung dịch nitroprusside (1 mg/ml được đưa vào qua tĩnh mạch đùi với tốc độ dòng chảy là 0,103 ml/phút) được truyền để đạt được sự giảm đáng kể MABP. Tại thời điểm này, động vật được tiến hành thiếu máu cục bộ trong 5 phút và tái tưới máu trong 10 phút bằng cách kẹp và mở kẹp động mạch cảnh chung bên phải. Trong toàn bộ thời gian thiếu máu cục bộ và tái tưới máu, ICP và MABP được theo dõi liên tục. Để cung cấp triển vọng về các biến đổi chuyển hóa của mô não xảy ra trong quá trình thiếu máu cục bộ và tái tưới máu, một số thông số sinh hóa của quá trình chuyển hóa năng lượng của tế bào và tổn thương màng do gốc oxy gây ra đã được xác định bằng phương pháp HPLC nhạy và có thể tái tạo trên các mô chiết xuất bằng axit pecloric. KẾT QUẢ VÀ KẾT LUẬN Tính hợp lệ của mô hình hiện tại được hỗ trợ bởi phát hiện về sự khác biệt đáng kể giữa các bán cầu trong nồng độ của một số hợp chất được coi là dấu ấn sinh hóa của tổn thương mô, chẳng hạn như chất chuyển hóa adenosine 5'-triphosphate và malondialdehyde, chất cuối cùng này chỉ ra tác động gây hại của oxy gốc tự do trên phospholipid màng tế bào."} +{"idx": 1950116, "en": "BACKGROUND Scattered reports of literature suggest the hypothesis that patients suffering from a severe stenosis of extracranial carotid artery may present an increased rate of intracranial berry aneurysms caused by the hemodynamic stress on the side opposite to the stenosis, namely on physiological shunts. However, this hypothesis has never been verified upon a large and homogeneous series focused on the argument. MATERIALS AND METHODS We reviewed a consecutive series of 405 patients submitted to carotid endarterectomy for stenosis greater or equal to 70%. RESULTS Thirteen aneurysm were found in 11 patients (2.6%). Our patients showed a slight increase of incidence regards to general population (1%), maybe due to the large number of aged patients among our subpopulation. Preoperative TCD evaluation showed the presence of increased flow velocities in the physiological shunts, namely the anterior cerebral artery and the anterior communicating artery, in 65% of the patients, and angiography confirmed the redistribution of intracranial circulation. Nevertheless, according to aneurysm location, no statistically significant correlation (0.3 < p < 0.4) was found between the presence of an aneurysm and the values of velocity in these arteries. CONCLUSIONS As regards the etiology of berry aneurysms, these data suggest that increased hemodynamic stress per se is not sufficient to cause the origin of berry aneurysms. Maybe, a role is possible only if either congenital, or acquired and age-related factor, peculiar of cerebral arteries, coexist.", "gemini": "BỐI CẢNH Các báo cáo rải rác trong tài liệu khoa học gợi ý giả thuyết rằng những bệnh nhân mắc chứng hẹp động mạch cảnh ngoài sọ nghiêm trọng có thể có tỷ lệ phình động mạch quả mọng nội sọ tăng do căng thẳng huyết động ở phía đối diện với hẹp, cụ thể là trên các đường tắt sinh lý. Tuy nhiên, giả thuyết này chưa bao giờ được xác minh trên một loạt các nghiên cứu lớn và đồng nhất tập trung vào lập luận. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi đã xem xét một loạt 405 bệnh nhân liên tiếp được phẫu thuật nội mạc động mạch cảnh vì hẹp lớn hơn hoặc bằng 70%. KẾT QUẢ Đã tìm thấy mười ba phình động mạch ở 11 bệnh nhân (2,6%). Bệnh nhân của chúng tôi cho thấy tỷ lệ mắc bệnh tăng nhẹ so với dân số nói chung (1%), có thể là do số lượng lớn bệnh nhân lớn tuổi trong nhóm dân số phụ của chúng tôi. Đánh giá TCD trước phẫu thuật cho thấy sự hiện diện của vận tốc dòng chảy tăng trong các đường tắt sinh lý, cụ thể là động mạch não trước và động mạch thông trước, ở 65% bệnh nhân và chụp mạch máu đã xác nhận sự phân phối lại tuần hoàn nội sọ. Tuy nhiên, theo vị trí phình động mạch, không tìm thấy mối tương quan có ý nghĩa thống kê (0,3 < p < 0,4) giữa sự hiện diện của phình động mạch và các giá trị vận tốc ở các động mạch này. KẾT LUẬN Đối với nguyên nhân gây ra phình động mạch quả mọng, những dữ liệu này cho thấy căng thẳng huyết động tăng tự nó không đủ để gây ra nguồn gốc của phình động mạch quả mọng. Có thể, một vai trò chỉ có thể xảy ra nếu các yếu tố bẩm sinh, hoặc mắc phải và liên quan đến tuổi tác, đặc thù của động mạch não cùng tồn tại."} +{"idx": 1950117, "en": "MATERIALS AND METHODS This study comprises a total of 322 patients who were seen in our neurosurgical department as inpatients (N = 241) or outpatients (N = 81) for lumbar disk herniation. Patients presenting with major neurological deficits and/or symptoms reflecting lumbar stenosis were excluded. All patients were scheduled for conservative treatment which was based on bedrest for a minimum of two weeks. Thereafter, patients were assigned for further nonoperative or operative treatment. RESULTS According to our results only 49% of conservatively treated patients had to be operated on. At long-term follow-up with a mean observation time of 2 years as available in 125 patients, 93% of patients who did not undergo surgery were improved with respect to pain. Motor function had recovered in 60%, and sensory deficits in 58% of these cases. CONCLUSIONS In conclusion, conservative treatment is effective and in our opinion mandatory in the setting of sciatica caused by lumbar disc herniation except for patients presenting with severe, or progressive neurological deficits.", "gemini": "VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu này bao gồm tổng cộng 322 bệnh nhân đã được khám tại khoa phẫu thuật thần kinh của chúng tôi với tư cách là bệnh nhân nội trú (N = 241) hoặc bệnh nhân ngoại trú (N = 81) vì thoát vị đĩa đệm thắt lưng. Những bệnh nhân có các khiếm khuyết thần kinh lớn và/hoặc các triệu chứng phản ánh hẹp ống sống thắt lưng đã bị loại trừ. Tất cả bệnh nhân đều được lên lịch điều trị bảo tồn dựa trên chế độ nghỉ ngơi trên giường trong tối thiểu hai tuần. Sau đó, bệnh nhân được chỉ định điều trị không phẫu thuật hoặc phẫu thuật. KẾT QUẢ Theo kết quả của chúng tôi, chỉ có 49% bệnh nhân được điều trị bảo tồn phải phẫu thuật. Trong quá trình theo dõi dài hạn với thời gian quan sát trung bình là 2 năm như có ở 125 bệnh nhân, 93% bệnh nhân không phẫu thuật đã cải thiện về cơn đau. Chức năng vận động đã phục hồi ở 60% và các khiếm khuyết về cảm giác ở 58% các trường hợp này. KẾT LUẬN Tóm lại, điều trị bảo tồn có hiệu quả và theo chúng tôi là bắt buộc trong trường hợp đau thần kinh tọa do thoát vị đĩa đệm thắt lưng, ngoại trừ những bệnh nhân có các khiếm khuyết thần kinh nghiêm trọng hoặc tiến triển."} +{"idx": 1950118, "en": "Evolution of psychological disorders following head injury including memory disorders and other cognitive ones are common. The best known are psychiatric disturbances of various kind after lesions of frontal lobes. Cognitive, behavioural and emotional disorders are not usually seen in patients with bilateral temporal lesions. In our Department of Neurotraumatology we observed 4 patients with post-traumatic lesions localized bitemporally. They developed Kluver-Bucy syndrome--rarity in human pathology--with combination of three or more the following syndromes: increased oral activity, hypersexuality, hypermetamorphosis, memory disorders, placidity, loss of people recognition, bulimia. Several symptoms responded dramatically to carbamazepine. We conclude that it may be a useful agent in treatment of this unusual syndrome.", "gemini": "Sự tiến triển của các rối loạn tâm lý sau chấn thương đầu bao gồm các rối loạn trí nhớ và các rối loạn nhận thức khác là phổ biến. Các rối loạn tâm thần nổi tiếng nhất có nhiều loại khác nhau sau các tổn thương ở thùy trán. Các rối loạn nhận thức, hành vi và cảm xúc thường không thấy ở những bệnh nhân bị tổn thương thái dương hai bên. Tại Khoa chấn thương thần kinh của chúng tôi, chúng tôi đã quan sát thấy 4 bệnh nhân bị tổn thương sau chấn thương định vị ở thái dương. Họ phát triển hội chứng Kluver-Bucy--hiếm gặp trong bệnh lý của con người--với sự kết hợp của ba hoặc nhiều hội chứng sau: tăng hoạt động miệng, tăng ham muốn tình dục, tăng chuyển hóa, rối loạn trí nhớ, điềm tĩnh, mất khả năng nhận biết người, chứng ăn vô độ. Một số triệu chứng đã đáp ứng đáng kể với carbamazepine. Chúng tôi kết luận rằng đây có thể là một tác nhân hữu ích trong điều trị hội chứng bất thường này."} +{"idx": 1950119, "en": "Two cases of association between intracranial meningioma and cerebral artery aneurysm are reported. A 50-year-old woman had a petroclival meningioma with a carotid-ophthalmic artery aneurysm; the surgery was performed first for the aneurysm then for the meningioma with good results. The second patient, a 67-year-old woman, had an olfactory meningioma with a carotid-posterior communicating artery aneurysm treated successfully in the same procedure. The pertinent literature is reviewed and the localization of meningiomas and aneurysms, the surgical treatment and the results at distance are discussed. It seems advisable, when possible, the contemporaneous treatment of the two lesions.", "gemini": "Hai trường hợp liên quan giữa u màng não nội sọ và phình động mạch não được báo cáo. Một phụ nữ 50 tuổi bị u màng não đá chẩm với phình động mạch cảnh-mắt; phẫu thuật được thực hiện đầu tiên cho phình động mạch sau đó cho u màng não với kết quả tốt. Bệnh nhân thứ hai, một phụ nữ 67 tuổi, bị u màng não khứu giác với phình động mạch thông nối cảnh-hậu được điều trị thành công trong cùng một thủ thuật. Tài liệu có liên quan được xem xét và vị trí của u màng não và phình động mạch, phương pháp điều trị phẫu thuật và kết quả ở xa được thảo luận. Có vẻ như nên điều trị đồng thời hai tổn thương khi có thể."} +{"idx": 1950120, "en": "The occurrence of a meningioma within the jugular foramen is very rare. We present the case of a 33-year-old man who presented with five-year history of hoarseness and was found to have paralysis of lower four cranial nerves on the left. High resolution computed tomography showed a dumbbell shaped tumor of the left jugular foramen extending intracranially over the jugular tubercle and extracranially into the parapharyngeal space. The patient underwent staged neuro-otological surgery and the mass was removed subtotally with no recurrence in six years. Histopathologic diagnosis was transitional meningioma. Our review of the literature revealed 33 previously reported cases. Four of these cases had neurofibromatosis. All but one underwent some form of otolaryngologic surgery. Although twenty had an intracranial component to their tumor, only 11 had a craniotomy. The majority of the tumors were meningothelial meningiomas (60%). Only two cases were malignant meningiomas. We reviewed the clinical and radiological characteristics and summarized the benefits and pitfalls of existing surgical options.", "gemini": "Việc xuất hiện khối u màng não trong lỗ tĩnh mạch cảnh rất hiếm. Chúng tôi trình bày trường hợp của một người đàn ông 33 tuổi, người đã trình bày với tiền sử khàn giọng trong năm năm và được phát hiện bị liệt bốn dây thần kinh sọ não dưới bên trái. Chụp cắt lớp vi tính độ phân giải cao cho thấy một khối u hình quả tạ của lỗ tĩnh mạch cảnh trái kéo dài trong sọ qua củ tĩnh mạch cảnh và ngoài sọ vào khoang sau họng. Bệnh nhân đã trải qua phẫu thuật thần kinh-tai theo giai đoạn và khối u đã được cắt bỏ gần như hoàn toàn mà không tái phát trong sáu năm. Chẩn đoán mô bệnh học là u màng não chuyển tiếp. Đánh giá tài liệu của chúng tôi cho thấy 33 trường hợp đã được báo cáo trước đó. Bốn trong số những trường hợp này mắc bệnh u xơ thần kinh. Tất cả trừ một trường hợp đã trải qua một số hình thức phẫu thuật tai mũi họng. Mặc dù hai mươi trường hợp có thành phần trong sọ đối với khối u của họ, chỉ có 11 trường hợp được cắt sọ. Phần lớn các khối u là u màng não màng não (60%). Chỉ có hai trường hợp là u màng não ác tính. Chúng tôi đã xem xét các đặc điểm lâm sàng và hình ảnh học và tóm tắt các lợi ích và nhược điểm của các lựa chọn phẫu thuật hiện có."} +{"idx": 1950121, "en": "A 66-year-old man presented to our Department with trigeminal neuralgia of the third division of the right trigeminal nerve. He also complained of some difficulty in mastication because of a tumour mass in the right lower jaw. A brain CT scan, X-rays of the lower jaw and viscerocranium CT scan revealed a malignant schwannoma of the right Gasserian ganglion involving simultaneously the distal end of the right mandibular nerve. The patient was operated on and has undergone a course of external irradiation. Seven years later, he is living a normal life. The value of the preoperative diagnosis of this extremely rare pathology is discussed because it seems that this tumour is radiosensitive.", "gemini": "Một người đàn ông 66 tuổi đến Khoa của chúng tôi với chứng đau dây thần kinh tam thoa ở nhánh thứ ba của dây thần kinh tam thoa bên phải. Ông cũng phàn nàn về một số khó khăn trong việc nhai do khối u ở hàm dưới bên phải. Chụp CT não, chụp X-quang hàm dưới và chụp CT sọ mặt cho thấy khối u ác tính của hạch Gasserian bên phải liên quan đồng thời đến đầu tận cùng của dây thần kinh hàm dưới bên phải. Bệnh nhân đã được phẫu thuật và đã trải qua một quá trình xạ trị bên ngoài. Bảy năm sau, anh ấy đang sống một cuộc sống bình thường. Giá trị của chẩn đoán trước phẫu thuật về bệnh lý cực kỳ hiếm gặp này được thảo luận vì có vẻ như khối u này nhạy cảm với tia xạ."} +{"idx": 1950122, "en": "A case is reported of ruptured posterior communicating artery aneurysm presenting as a painful ophthalmoplegia syndrome (spheno-cavernous syndrome). This case suggests that there is need to carried out immediately neuroradiological studies when a painful ophthalmoplegia appears because of possible underlying intracranial aneurysm. This decisional algorithm should decrease the probability of aneurysmal rebleeding and increase the chance of oculomotor nerve function recovery.", "gemini": "Một trường hợp vỡ phình động mạch thông nối sau biểu hiện là hội chứng liệt vận nhãn đau (hội chứng sphenoid-xoang hang) đã được báo cáo. Trường hợp này gợi ý rằng cần phải tiến hành các nghiên cứu thần kinh ngay lập tức khi xuất hiện liệt vận nhãn đau do có thể có phình động mạch trong sọ tiềm ẩn. Thuật toán quyết định này sẽ làm giảm khả năng xuất huyết lại của phình động mạch và tăng cơ hội phục hồi chức năng của dây thần kinh vận nhãn."} +{"idx": 1950123, "en": "The case is reported of a 58 year-old man with left-sided trigeminal and glossopharyngeal neuralgia, left hemifacial spasm and arterial hypertension. Preoperative MRI angiography examination showed a massively ectatic left vertebral artery. At operation a multiple neurovascular compression, which is described, was discovered. Microvascular decompression was performed and postoperatively the patient had complete relief from the trigeminal and glossopharyngeal neuralgia, from the hemifacial spasm, and has sustained normal arterial pressure without medication. The value of MRI in diagnosing of neurovascular compression syndromes and pathophysiology of the \"so called\" arterial hypertension are discussed.", "gemini": "Báo cáo trường hợp một người đàn ông 58 tuổi bị đau dây thần kinh tam thoa và dây thần kinh hầu họng bên trái, co thắt nửa mặt trái và tăng huyết áp động mạch. Kiểm tra chụp mạch cộng hưởng từ trước mổ cho thấy động mạch đốt sống trái giãn nở rất nhiều. Trong khi phẫu thuật, người ta phát hiện ra tình trạng chèn ép thần kinh mạch máu nhiều lần, được mô tả. Giải nén mạch máu vi phẫu đã được thực hiện và sau phẫu thuật, bệnh nhân đã khỏi hoàn toàn đau dây thần kinh tam thoa và dây thần kinh hầu họng, co thắt nửa mặt và duy trì huyết áp bình thường mà không cần dùng thuốc. Giá trị của MRI trong chẩn đoán hội chứng chèn ép thần kinh mạch máu và bệnh sinh của \"cái gọi là\" tăng huyết áp động mạch được thảo luận."} +{"idx": 1950124, "en": "The authors describe a iatrogenic cerebrospinal fluid fistula into the pleural cavity, a rare and unusual complication of thoracic surgery. The importance of considering this diagnosis in patients who suffer from headache and altered mental status after thoracotomy and the early surgical repair of this potentially fatal complication are stressed.", "gemini": "Các tác giả mô tả một lỗ rò dịch não tủy do chấn thương vào khoang màng phổi, một biến chứng hiếm gặp và bất thường của phẫu thuật ngực. Tầm quan trọng của việc xem xét chẩn đoán này ở những bệnh nhân bị đau đầu và thay đổi trạng thái tinh thần sau phẫu thuật cắt bỏ thùy phổi và việc phẫu thuật sớm để khắc phục biến chứng có khả năng gây tử vong này được nhấn mạnh."} +{"idx": 1950125, "en": "A low cost method for providing continuous saline drip in bipolar diathermy is described, which use is adaptable to any type of standard bipolar forceps. The advantages of this system versus commercially available self-irrigating forceps are reported.", "gemini": "Một phương pháp chi phí thấp để cung cấp dịch muối nhỏ giọt liên tục trong phẫu thuật điện cực lưỡng cực được mô tả, có thể sử dụng được cho bất kỳ loại kìm lưỡng cực tiêu chuẩn nào. Các ưu điểm của hệ thống này so với kìm tự tưới có sẵn trên thị trường được báo cáo."} +{"idx": 1950126, "en": "Diagnosis of periodontal disease progression involves recording two probing attachment level measurements over an adequate time interval. A diagnostic instrument which exhibits less measurement variability allows for increased sensitivity and earlier disease detection. Traditionally, a manual probe with an occlusal stent of the cementoenamel junction (CEJ) as a reference landmark has been the method of choice. Automated probes that use an occlusal disk as the reference landmark have been developed as an alternative means of measure. The aim of this study was to compare the variability of these two probing methods. Four hundred eleven (411) interproximal sites in 46 untreated periodontitis patients were monitored by a single examiner over a 6-month period. Each site was measured on a monthly basis, first with an automated probe (AP) followed by a manual probe (MP) in combination with a custom-fabricated acrylic stent. Measurement variability of the two probing methods was also compared over a 7-day interval. The AP measurements were significantly more variable than the MP measurements (P < 0.001) when considering the variability between two passes at the same visit. Over the 6-month period, the MP measurements demonstrated significantly more variability than the AP measurements (P < 0.001). It was also noted that MP measurements exhibited more variability at sites with frequent bleeding during the 6 months of the study (P = 0.006). The results of this study demonstrate that AP may have less variability of attachment level measurements over a 6-month period and may be less influenced by local inflammatory changes. However, future comparison studies should include multiple examiners to reduce examiner bias and should alternate the probing method to reduce bias created by local tissue changes from multiple probings.", "gemini": "Chẩn đoán tiến triển bệnh nha chu liên quan đến việc ghi lại hai phép đo mức độ bám dính thăm dò trong một khoảng thời gian đầy đủ. Một dụng cụ chẩn đoán có độ biến thiên khi đo nhỏ hơn cho phép tăng độ nhạy và phát hiện bệnh sớm hơn. Theo truyền thống, đầu dò thủ công với máng cắn của mối nối men-xi măng (CEJ) làm mốc tham chiếu đã là phương pháp được lựa chọn. Đầu dò tự động sử dụng đĩa cắn làm mốc tham chiếu đã được phát triển như một phương tiện đo lường thay thế. Mục đích của nghiên cứu này là so sánh độ biến thiên của hai phương pháp thăm dò này. Bốn trăm mười một (411) vị trí kẽ răng ở 46 bệnh nhân viêm nha chu chưa được điều trị đã được một bác sĩ duy nhất theo dõi trong khoảng thời gian 6 tháng. Mỗi vị trí được đo hàng tháng, đầu tiên bằng đầu dò tự động (AP) sau đó bằng đầu dò thủ công (MP) kết hợp với máng acrylic tùy chỉnh. Độ biến thiên khi đo của hai phương pháp thăm dò cũng được so sánh trong khoảng thời gian 7 ngày. Các phép đo AP có độ biến thiên đáng kể hơn so với các phép đo MP (P < 0,001) khi xem xét độ biến thiên giữa hai lần đo trong cùng một lần khám. Trong khoảng thời gian 6 tháng, các phép đo MP cho thấy độ biến thiên đáng kể hơn so với các phép đo AP (P < 0,001). Người ta cũng lưu ý rằng các phép đo MP thể hiện nhiều biến thiên hơn ở các vị trí chảy máu thường xuyên trong 6 tháng nghiên cứu (P = 0,006). Kết quả của nghiên cứu này chứng minh rằng AP có thể có độ biến thiên ít hơn về các phép đo mức độ bám dính trong khoảng thời gian 6 tháng và có thể ít bị ảnh hưởng bởi các thay đổi viêm cục bộ. Tuy nhiên, các nghiên cứu so sánh trong tương lai nên bao gồm nhiều bác sĩ để giảm sai số của bác sĩ và nên thay đổi phương pháp thăm dò để giảm sai số do các thay đổi mô cục bộ từ nhiều lần thăm dò."} +{"idx": 1950127, "en": "This study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube-fed children and 24 healthy controls. Microbial identification was carried out using anaerobic culture and 16S rRNA-based PCR identification methods. Streptococcal and Actinomyces species were recovered from 100% and 76% of all subjects and averaged 66% and 11% of total cultivable organisms, respectively. In decreasing order of prevalence, Fusobacterium, enteric rods, Prevotella intermedia/Prevotella nigrescens, Capnocytophaga, Propionibacterium, yeasts, Actinobacillus actinomycetemcomitans, coagulase-negative Staphylococcus, Campylobacter rectus, Bacteroides forsythus, and Porphyromonas gingivalis were detected in 48% to 2% of the study subjects. The cultivable levels of these species varied widely among subjects. PCR detection showed C. rectus and Eikenella corrodens both to occur in 93% of the study subjects and to be the most prevalent putative periodontal pathogens examined. In decreasing order of prevalence, PCR identified Treponema denticola, A. actinomycetemcomitans, P. nigrescens, P. intermedia, B. forsythus, and P. gingivalis in 38% to 21% of the subjects studied. Tube-fed children and healthy controls exhibited similar subgingival microbial compositions. It appears from this study that oral food consumption is not a major determinant for the establishment of subgingival microbiota in children.", "gemini": "Nghiên cứu này đã kiểm tra tác động của việc tiêu thụ thức ăn qua đường miệng đối với tỷ lệ lưu hành và mức độ vi khuẩn và nấm men dưới nướu ở 20 trẻ em được nuôi bằng ống thông dạ dày và 24 trẻ đối chứng khỏe mạnh. Việc xác định vi sinh vật được thực hiện bằng phương pháp nuôi cấy kỵ khí và phương pháp xác định dựa trên PCR rRNA 16S. Các loài Streptococcus và Actinomyces được phục hồi từ 100% và 76% tất cả các đối tượng và trung bình chiếm lần lượt là 66% và 11% tổng số vi sinh vật có thể nuôi cấy được. Theo thứ tự giảm dần về tỷ lệ lưu hành, Fusobacterium, trực khuẩn ruột, Prevotella intermedia/Prevotella nigrescens, Capnocytophaga, Propionibacterium, nấm men, Actinobacillus actinomycetemcomitans, tụ cầu âm tính với coagulase, Campylobacter rectus, Bacteroides forsythus và Porphyromonas gingivalis đã được phát hiện ở 48% đến 2% đối tượng nghiên cứu. Mức độ có thể nuôi cấy của các loài này thay đổi rất nhiều giữa các đối tượng. Phát hiện PCR cho thấy C. rectus và Eikenella corrodens đều xuất hiện ở 93% đối tượng nghiên cứu và là những tác nhân gây bệnh nha chu được cho là phổ biến nhất. Theo thứ tự giảm dần về tỷ lệ lưu hành, PCR xác định được Treponema denticola, A. actinomycetemcomitans, P. nigrescens, P. intermedia, B. forsythus và P. gingivalis ở 38% đến 21% đối tượng được nghiên cứu. Trẻ em được nuôi bằng ống thông và trẻ đối chứng khỏe mạnh có thành phần vi sinh vật dưới nướu tương tự nhau. Nghiên cứu này cho thấy rằng việc tiêu thụ thức ăn qua đường miệng không phải là yếu tố chính quyết định sự hình thành hệ vi sinh vật dưới nướu ở trẻ em."} +{"idx": 1950128, "en": "The present study was designed to compare the amount and regional distribution of bone formation around hydroxyapatite (HA) implants in normal (control) rats with that of animals with diabetes mellitus (DM), induced by streptozotocin 2 weeks prior to implant placement. Calcein (CAL), alizarin complexone (AL), and tetracycline (TC) were injected on the 7th, 14th, and 21st days after implantation, respectively, and the rats were sacrificed on the 28th day after implantation. Seventy-microns undecalcified sections of the HA-bone interface in both groups were then prepared for confocal laser scanning microscopy (CLSM) observation. In both groups, bone formation developed from the HA surface to the endosteum, periosteum, or bone marrow. In the control group, around the HA close to the endosteum and periosteum, the new bone showed an extensive lamination pattern of three color layers (CAL, AL, and TC), but in the DM group the labeling density of TC on the 21st day was low. In contrast, on the lateral part of the HA surface (away from the endosteum and periosteum), there was considerably less bone formation in the control group, and in the DM group it was almost completely suppressed. These findings indicate that bone formation around the HA was initiated from the HA surface in the control group, while in the DM group, bone formation along the lateral part of the HA away from the endosteum and periosteum was almost completely suppressed. Furthermore, it is also suggested that in the new bone along the HA close to the endosteum and periosteum, only calcification on the 21st day was depressed.", "gemini": "Nghiên cứu hiện tại được thiết kế để so sánh lượng và phân bố khu vực của sự hình thành xương xung quanh các implant hydroxyapatite (HA) ở chuột bình thường (kiểm soát) với động vật bị đái tháo đường (ĐTĐ), được gây ra bởi streptozotocin 2 tuần trước khi đặt implant. Calcein (CAL), phức hợp alizarin (AL) và tetracycline (TC) được tiêm lần lượt vào ngày thứ 7, 14 và 21 sau khi cấy ghép và chuột được hy sinh vào ngày thứ 28 sau khi cấy ghép. Các phần không khử khoáng 70 micromet của giao diện xương-HA ở cả hai nhóm sau đó được chuẩn bị để quan sát bằng kính hiển vi quét laser cộng hưởng (CLSM). Ở cả hai nhóm, sự hình thành xương phát triển từ bề mặt HA đến màng xương, màng xương hoặc tủy xương. Ở nhóm đối chứng, xung quanh HA gần màng xương và màng xương, xương mới cho thấy kiểu phiến rộng ba lớp màu (CAL, AL và TC), nhưng ở nhóm ĐTĐ, mật độ đánh dấu của TC vào ngày thứ 21 thấp. Ngược lại, ở phần bên của bề mặt HA (xa màng xương và màng xương), sự hình thành xương ít hơn đáng kể ở nhóm đối chứng và ở nhóm ĐTĐ, nó gần như bị ức chế hoàn toàn. Những phát hiện này chỉ ra rằng sự hình thành xương xung quanh HA bắt đầu từ bề mặt HA ở nhóm đối chứng, trong khi ở nhóm ĐTĐ, sự hình thành xương dọc theo phần bên của HA xa màng xương và màng xương gần như bị ức chế hoàn toàn. Hơn nữa, người ta cũng cho rằng ở xương mới dọc theo HA gần màng xương và màng xương, chỉ có quá trình vôi hóa vào ngày thứ 21 bị ức chế."} +{"idx": 1950129, "en": "The main purpose of the study was to examine the anti-malodor properties of oxidizing lozenges, as compared to breath mints and chewing gum. Healthy, young adult volunteers (N = 123; mean age 24.5 years) were measured for oral malodor-related parameters (whole mouth odor measured by 2 judges; tongue dorsum posterior odor using the spoon test; volatile sulphide levels; salivary levels of cadaverine and putrescine; and 2 versions of an oral rinse test) on the first afternoon of the study. They were then assigned randomly to one of 6 groups (2 brands of breath mints, chewing gum with no active ingredients, regular and full-strength oxidizing lozenges, and a no-treatment control), and instructed to employ the treatment before bedtime, the next morning, and in the early afternoon 3 hours prior to measurements, which were carried out 24 hours following baseline measurements. Volunteers also estimated the level of their own whole mouth and tongue odors at baseline and post-treatment. The data showed that, among treatments, only the full-strength oxidizing lozenge significantly reduced tongue dorsum malodor, as determined by the spoon test. The full-strength lozenge also yielded a significant increase in the modified oral rinse test, presumably due, at least in part, to residual oxidizing activity retained in the oral cavity. Self-estimations of whole mouth and tongue malodor by volunteers were significantly correlated with corresponding-judge assessments, suggesting some degree of objectivity in assessing one's own oral malodor.", "gemini": "Mục đích chính của nghiên cứu là kiểm tra các đặc tính khử mùi hôi của viên ngậm oxy hóa, so với kẹo bạc hà và kẹo cao su. Các tình nguyện viên là người lớn trẻ tuổi khỏe mạnh (N = 123; tuổi trung bình 24,5 tuổi) được đo các thông số liên quan đến mùi hôi miệng (mùi toàn bộ miệng do 2 giám khảo đo; mùi mặt sau của lưỡi bằng cách sử dụng thìa thử; mức độ sunfua dễ bay hơi; mức độ cadaverine và putrescine trong nước bọt; và 2 phiên bản của bài kiểm tra súc miệng) vào buổi chiều đầu tiên của nghiên cứu. Sau đó, họ được phân ngẫu nhiên vào một trong 6 nhóm (2 nhãn hiệu kẹo bạc hà, kẹo cao su không có thành phần hoạt tính, viên ngậm oxy hóa thông thường và viên ngậm oxy hóa nồng độ đầy đủ và nhóm đối chứng không điều trị) và được hướng dẫn sử dụng phương pháp điều trị trước khi đi ngủ, sáng hôm sau, và vào đầu buổi chiều trước 3 giờ đo, được thực hiện sau 24 giờ so với các phép đo ban đầu. Các tình nguyện viên cũng ước tính mức độ mùi hôi miệng toàn bộ và lưỡi của chính họ trước khi điều trị và sau điều trị. Dữ liệu cho thấy, trong số các phương pháp điều trị, chỉ có viên ngậm oxy hóa nồng độ đầy đủ mới làm giảm đáng kể mùi hôi ở mặt sau của lưỡi, theo xác định của thìa thử. Viên ngậm nồng độ đầy đủ cũng mang lại sự gia tăng đáng kể trong bài kiểm tra súc miệng đã sửa đổi, có lẽ ít nhất một phần là do hoạt động oxy hóa còn lại trong khoang miệng. Tự đánh giá mùi hôi miệng toàn bộ và lưỡi của các tình nguyện viên có tương quan đáng kể với đánh giá của giám khảo tương ứng, cho thấy một mức độ khách quan nào đó trong việc đánh giá mùi hôi miệng của chính mình."} +{"idx": 1950130, "en": "Halitosis is known as unpleasant oral odor and is a health concern among the general public. Previously, we reported on a new portable monitor with a zinc-oxide, thin-film, semiconductor sensor which demonstrated simplicity of handling, high reproducibility and correspondence for organoleptic assessment. The results suggested its usefulness for the diagnosis of halitosis. Using the monitor, oral air samples of 94 subjects were measured in a field survey, and the values were compared with the organoleptic rates of corresponding samples assessed by two judges. A highly significant correlation (r = 0.824, P < 0.01) was demonstrated between the measures obtained by the two methods. The results suggest that the monitor is useful for not only a clinical study but also a field study of halitosis.", "gemini": "Hôi miệng được biết đến là mùi hôi khó chịu trong miệng và là mối quan tâm về sức khỏe của công chúng. Trước đây, chúng tôi đã báo cáo về một thiết bị giám sát cầm tay mới có cảm biến bán dẫn màng mỏng oxit kẽm, thiết bị này thể hiện tính đơn giản khi sử dụng, khả năng tái tạo cao và sự tương ứng để đánh giá bằng cảm quan. Kết quả cho thấy tính hữu ích của thiết bị này đối với chẩn đoán hôi miệng. Sử dụng thiết bị giám sát, các mẫu không khí trong miệng của 94 đối tượng đã được đo trong một cuộc khảo sát thực địa và các giá trị này được so sánh với tỷ lệ cảm quan của các mẫu tương ứng do hai giám khảo đánh giá. Một mối tương quan rất có ý nghĩa (r = 0,824, P < 0,01) đã được chứng minh giữa các phép đo thu được bằng hai phương pháp. Kết quả cho thấy thiết bị giám sát hữu ích không chỉ cho một nghiên cứu lâm sàng mà còn cho một nghiên cứu thực địa về hôi miệng."} +{"idx": 1950131, "en": "This paper addresses the diagnostic and therapeutic implications of increased probing depths. In general, in untreated and treated patients, when deep and shallow probing depths are compared, the data indicate that deep sites are associated with increased bleeding upon probing, elevated subgingival temperatures, higher levels of pathogens, more probing errors, a greater amount of infiltrated connective tissue, reduced ability to remove subgingival deposits with root planing, and diminished effectiveness of oral hygiene to alter the subgingival microbiota. Clinical trials demonstrate that probing depth is not a good predictor of future disease progression. However, deep sites are at greater risk of disease progression than shallow sites in untreated and treated patients. Furthermore, the deeper the probing depth, the greater the risk of future disease progression. Overall, the preponderance of evidence indicates that it is advantageous, but not always necessary, for patients to have shallow probing depths. With regards to surgical reduction of probing depths beyond that attained with non-surgical therapy, clinicians need to consider the advantages (e.g., ease of maintenance, reduced risk of disease progression) and disadvantages (e.g., root sensitivity, cosmetic defects) of treatment procedures. Since numerous variables require consideration (e.g., response to root planing, goals of therapy, acceptable level of risk for future disease progression), treatment decisions will vary depending on the patient and the desired clinical outcome at specific sites.", "gemini": "Bài báo này đề cập đến các tác động chẩn đoán và điều trị của việc tăng độ sâu thăm dò. Nói chung, ở những bệnh nhân chưa điều trị và đã điều trị, khi so sánh độ sâu thăm dò sâu và nông, dữ liệu chỉ ra rằng các vị trí sâu có liên quan đến tình trạng chảy máu nhiều hơn khi thăm dò, nhiệt độ dưới nướu tăng cao, mức độ mầm bệnh cao hơn, nhiều lỗi thăm dò hơn, số lượng mô liên kết xâm nhập nhiều hơn, khả năng loại bỏ cặn dưới nướu bằng dụng cụ cạo vôi răng giảm và hiệu quả vệ sinh răng miệng giảm để thay đổi hệ vi sinh vật dưới nướu. Các thử nghiệm lâm sàng chứng minh rằng độ sâu thăm dò không phải là yếu tố dự báo tốt về tiến triển bệnh trong tương lai. Tuy nhiên, các vị trí sâu có nguy cơ tiến triển bệnh cao hơn so với các vị trí nông ở những bệnh nhân chưa điều trị và đã điều trị. Hơn nữa, độ sâu thăm dò càng sâu thì nguy cơ tiến triển bệnh trong tương lai càng lớn. Nhìn chung, phần lớn các bằng chứng chỉ ra rằng có lợi, nhưng không phải lúc nào cũng cần thiết, để bệnh nhân có độ sâu thăm dò nông. Đối với việc giảm độ sâu thăm dò bằng phẫu thuật vượt quá mức đạt được bằng liệu pháp không phẫu thuật, các bác sĩ lâm sàng cần cân nhắc những ưu điểm (ví dụ: dễ bảo dưỡng, giảm nguy cơ tiến triển bệnh) và nhược điểm (ví dụ: răng nhạy cảm, khiếm khuyết thẩm mỹ) của các thủ thuật điều trị. Vì cần xem xét nhiều biến số (ví dụ: đáp ứng với việc cạo vôi răng, mục tiêu điều trị, mức độ rủi ro chấp nhận được đối với tiến triển bệnh trong tương lai), nên các quyết định điều trị sẽ khác nhau tùy thuộc vào bệnh nhân và kết quả lâm sàng mong muốn tại các vị trí cụ thể."} +{"idx": 1950132, "en": "The tissue penetration of azithromycin, the prototype of a new class of macrolide antibiotics named azalides, was studied in patients undergoing surgery for third-molar removal. Drug concentrations in plasma, saliva, and periodontal tissues were evaluated in 28 patients treated with azithromycin 500 mg/day per os for 3 consecutive days. Samples of blood, saliva, gingiva, and alveolar bone were collected during oral surgery, 12 hours, and 2.5, 4.5, and 6.5 days after the last dosing, and the azithromycin concentration was measured microbiologically by using Micrococcus luteus NCTC 8440 as the reference organism. The highest concentrations of azithromycin were observed 12 hours after the last dose in plasma, saliva, gingiva, and bone (0.33 +/- 0.04 mg/l, 2.14 +/- 0.30 mg/l, 6.47 +/- 0.57 mg/kg, and 1.86 +/- 0.15 mg/kg, respectively) and then declined gradually. However, consistent levels of the drug in saliva and periodontal tissues could be detected up to 6.5 days, indicating that azithromycin was retained in target tissues and fluids for a long time after the end of treatment. Among the samples examined, the highest concentration of azithromycin was found in the gingiva at each time studied. Moreover, the ratios of salivary or periodontal tissue levels versus plasma concentrations remained nearly unmodified from 12 hours up to 6.5 days. Overall, these results indicate a favorable disposition of azithromycin into saliva and periodontal tissues and suggest that this macrolide antibiotic represents a valuable option in the pharmacologic treatment of odontogenic infections.", "gemini": "Sự thâm nhập mô của azithromycin, nguyên mẫu của một nhóm kháng sinh macrolide mới được gọi là azalides, đã được nghiên cứu ở những bệnh nhân phẫu thuật cắt bỏ răng hàm thứ ba. Nồng độ thuốc trong huyết tương, nước bọt và mô nha chu được đánh giá ở 28 bệnh nhân được điều trị bằng azithromycin 500 mg/ngày uống trong 3 ngày liên tiếp. Các mẫu máu, nước bọt, nướu răng và xương ổ răng được thu thập trong quá trình phẫu thuật răng miệng, 12 giờ và 2,5, 4,5 và 6,5 ngày sau khi dùng liều cuối cùng, và nồng độ azithromycin được đo bằng phương pháp vi sinh học sử dụng Micrococcus luteus NCTC 8440 làm sinh vật tham chiếu. Nồng độ azithromycin cao nhất được quan sát thấy 12 giờ sau liều cuối cùng trong huyết tương, nước bọt, nướu răng và xương (lần lượt là 0,33 +/- 0,04 mg/l, 2,14 +/- 0,30 mg/l, 6,47 +/- 0,57 mg/kg và 1,86 +/- 0,15 mg/kg), sau đó giảm dần. Tuy nhiên, nồng độ thuốc ổn định trong nước bọt và mô nha chu có thể được phát hiện đến 6,5 ngày, cho thấy azithromycin được giữ lại trong mô và dịch đích trong một thời gian dài sau khi kết thúc điều trị. Trong số các mẫu được kiểm tra, nồng độ azithromycin cao nhất được tìm thấy ở nướu răng tại mỗi thời điểm nghiên cứu. Hơn nữa, tỷ lệ nồng độ nước bọt hoặc mô nha chu so với nồng độ huyết tương vẫn gần như không thay đổi từ 12 giờ đến 6,5 ngày. Nhìn chung, những kết quả này cho thấy sự phân bố thuận lợi của azithromycin vào nước bọt và mô nha chu và cho thấy rằng loại kháng sinh macrolide này là một lựa chọn có giá trị trong điều trị dược lý các bệnh nhiễm trùng nha khoa."} +{"idx": 1950133, "en": "The purpose of this investigation was to determine whether the presence of disease-associated bacteria in health-associated plaque correlated with susceptibility to periodontitis over time. Sites of periodontal health were identified in 65 adults. Six months later (recall 1), plaque was collected from sites that remained in periodontal health, and specific bacteria were detected using monoclonal antibodies in a microscopic assay. The spirochete morphogroup was identified by phase contrast microscopy. The relationship between detection at recall 1 and development of periodontitis over two successive 6-month intervals (recalls 2 and 3) was evaluated by means of logistic regression using generalized estimating equations (GEE), from which odds ratios (OR) were estimated and tested for significance. Significant relationships were defined as those having ORs with P < 0.05. Ninety-three of 1,032 sites developed signs of early periodontitis over the 12-month interval between recall 1 and recall 3. The spirochete morphogroup (OR = 3.13, P < 0.001) and pathogen-related oral spirochetes (PROS) (OR = 3.68, P < 0.001) were significantly associated with healthy sites that developed periodontitis. The association of Treponema socranskii was not significant (OR = 3.62, P = 0.0918). Odds ratios for Campylobacter rectus, Eikenella corrodens, and Porphyromonas gingivalis were less than 2.0 and not significant. Treponema denticola was not detected in health-associated plaque from stable health sites and was detected in only three sites that progressed to periodontitis. These findings indicate that the presence of PROS and some unidentified spirochetes in health-associated plaque is associated with increased susceptibility to periodontitis.", "gemini": "Mục đích của cuộc điều tra này là xác định xem sự hiện diện của vi khuẩn liên quan đến bệnh trong mảng bám liên quan đến sức khỏe có tương quan với tình trạng dễ mắc bệnh nha chu theo thời gian hay không. Các vị trí sức khỏe nha chu đã được xác định ở 65 người lớn. Sáu tháng sau (lần khám lại 1), mảng bám được thu thập từ các vị trí vẫn còn sức khỏe nha chu và các vi khuẩn cụ thể được phát hiện bằng cách sử dụng kháng thể đơn dòng trong xét nghiệm kính hiển vi. Nhóm hình thái xoắn khuẩn được xác định bằng kính hiển vi tương phản pha. Mối quan hệ giữa phát hiện tại lần khám lại 1 và sự phát triển của bệnh nha chu trong hai khoảng thời gian 6 tháng liên tiếp (lần khám lại 2 và 3) được đánh giá bằng phương pháp hồi quy logistic sử dụng các phương trình ước lượng tổng quát (GEE), từ đó tỷ lệ cược (OR) được ước tính và kiểm định độ có ý nghĩa. Các mối quan hệ có ý nghĩa được định nghĩa là những mối quan hệ có OR có P < 0,05. Chín mươi ba trong số 1.032 vị trí đã phát triển các dấu hiệu của bệnh nha chu sớm trong khoảng thời gian 12 tháng giữa lần khám lại 1 và lần khám lại 3. Nhóm hình thái xoắn khuẩn (OR = 3,13, P < 0,001) và xoắn khuẩn miệng liên quan đến mầm bệnh (PROS) (OR = 3,68, P < 0,001) có liên quan đáng kể đến các vị trí khỏe mạnh phát triển bệnh nha chu. Sự liên quan của Treponema socranskii không có ý nghĩa (OR = 3,62, P = 0,0918). Tỷ lệ cược đối với Campylobacter rectus, Eikenella corrodens và Porphyromonas gingivalis nhỏ hơn 2,0 và không có ý nghĩa. Treponema denticola không được phát hiện trong mảng bám liên quan đến sức khỏe từ các vị trí sức khỏe ổn định và chỉ được phát hiện ở ba vị trí tiến triển thành bệnh nha chu. Những phát hiện này chỉ ra rằng sự hiện diện của PROS và một số xoắn khuẩn chưa xác định trong mảng bám liên quan đến sức khỏe có liên quan đến tình trạng dễ mắc bệnh nha chu hơn."} +{"idx": 1950134, "en": "An in vitro comparative study was conducted in order to evaluate the effects of an air-abrasive system on dental implant surfaces. Eight new titanium dental implants, four standard machined implants (machined group), and four standard plasma-sprayed implants (plasma-sprayed group) were selected for investigation. Both neck and body surfaces of the implants were analyzed. Each pair of implants in each group was treated as follows: the spray of the air-abrasive unit was applied to each area for 5 seconds on the first implant and 15 seconds on the second implant. A total of 24 areas were observed: 16 test implants and 8 controls. Scanning electron photomicrographs were analyzed by 3 examiners using a category rating scale (kappa = 0.594). The images were also computerized for texture analysis. The results indicate that a single air-powder abrasive treatment of the dental implants selected for this study modified their exposed surfaces. After treatment, the threaded neck surface of a machined group implant was least affected, whereas the body was the most altered. In the plasma-sprayed group, comparisons between implant surfaces showed little change. In the machined group, more change was observed in both neck and body areas. In all specimens, a 5-second exposure to the air-powder abrasive did not induce deep changes in the surfaces. A 15-second exposure modified all the specimen surfaces. Further studies are needed to evaluate the effect of these changes on the biological osseointegration process.", "gemini": "Một nghiên cứu so sánh trong ống nghiệm đã được tiến hành để đánh giá tác động của hệ thống phun khí lên bề mặt implant nha khoa. Tám implant nha khoa bằng titan mới, bốn implant gia công tiêu chuẩn (nhóm gia công) và bốn implant phun plasma tiêu chuẩn (nhóm phun plasma) đã được chọn để nghiên cứu. Cả bề mặt cổ và thân của implant đều được phân tích. Mỗi cặp implant trong mỗi nhóm được xử lý như sau: phun của bộ phận phun khí được áp dụng cho mỗi vùng trong 5 giây trên implant đầu tiên và 15 giây trên implant thứ hai. Tổng cộng 24 vùng được quan sát: 16 implant thử nghiệm và 8 implant đối chứng. Ảnh chụp điện tử quét được phân tích bởi 3 giám định viên sử dụng thang đánh giá theo thể loại (kappa = 0,594). Các hình ảnh cũng được vi tính hóa để phân tích kết cấu. Kết quả cho thấy một phương pháp xử lý mài mòn không khí-bột duy nhất của các implant nha khoa được chọn cho nghiên cứu này đã làm thay đổi bề mặt tiếp xúc của chúng. Sau khi xử lý, bề mặt cổ có ren của implant nhóm gia công bị ảnh hưởng ít nhất, trong khi thân bị thay đổi nhiều nhất. Trong nhóm phun plasma, sự so sánh giữa các bề mặt implant cho thấy ít thay đổi. Ở nhóm gia công, sự thay đổi được quan sát nhiều hơn ở cả vùng cổ và vùng thân. Ở tất cả các mẫu, phơi nhiễm 5 giây với chất mài mòn không khí-bột không gây ra những thay đổi sâu ở bề mặt. Phơi nhiễm 15 giây đã làm thay đổi tất cả các bề mặt mẫu. Cần có thêm các nghiên cứu để đánh giá tác động của những thay đổi này đối với quá trình tích hợp xương sinh học."} +{"idx": 1950135, "en": "The inter-incisal distance and dimension of the interdental papilla between the mandibular incisors were examined in cyclosporin A (CSA) fed rats over 6 weeks. Rats in the test group received CSA daily in mineral oil by gastric feeding (30 mg/kg body weight); the control group received mineral oil only. The inter-incisal distance and gingival dimensions, including bucco-lingual width and vertical height, were assessed biweekly from alginate impressed stone models. Animals were sacrificed at the end of the study and tissue sections were obtained from the anterior region of the mandible for histopathological evaluation. Both the inter-incisal distance and the dimension of the interdental papilla were significantly greater in CSA-exposed animals compared to control. The significant alteration appeared earlier in the papillary dimensions than that in the interdental distance. Particular histopathological alterations of the soft and hard tissues of the periodontium were observed in CSA-exposed animals. Within limitations of the study, we suggest that the CSA-induced gingival overgrowth may offer an active force contributing to observed tooth movement, however, remarkable alveolar remodeling should be considered as an undetermined factor for the movement.", "gemini": "Khoảng cách giữa các răng cửa và kích thước của nhú giữa các răng ở răng cửa hàm dưới được kiểm tra ở những con chuột được cho ăn cyclosporin A (CSA) trong hơn 6 tuần. Những con chuột trong nhóm thử nghiệm được cho ăn CSA hàng ngày trong dầu khoáng bằng cách cho ăn qua dạ dày (30 mg/kg trọng lượng cơ thể); nhóm đối chứng chỉ được cho ăn dầu khoáng. Khoảng cách giữa các răng cửa và kích thước nướu, bao gồm chiều rộng má-lưỡi và chiều cao thẳng đứng, được đánh giá hai tuần một lần từ các mô hình bằng đá được tạo hình bằng alginate. Động vật được hy sinh vào cuối nghiên cứu và các phần mô được lấy từ vùng trước của xương hàm dưới để đánh giá mô bệnh học. Khoảng cách giữa các răng cửa và kích thước của nhú giữa các răng đều lớn hơn đáng kể ở những động vật tiếp xúc với CSA so với nhóm đối chứng. Sự thay đổi đáng kể xuất hiện sớm hơn ở kích thước nhú hơn là ở khoảng cách giữa các răng. Những thay đổi mô bệnh học đặc biệt của các mô mềm và cứng của nha chu được quan sát thấy ở những động vật tiếp xúc với CSA. Trong phạm vi hạn chế của nghiên cứu, chúng tôi cho rằng sự phát triển quá mức của nướu do CSA gây ra có thể tạo ra một lực chủ động góp phần vào chuyển động của răng được quan sát thấy, tuy nhiên, sự tái tạo ổ răng đáng kể nên được coi là một yếu tố chưa xác định đối với chuyển động."} +{"idx": 1950136, "en": "This paper is one of three in a series prepared by the Research, Science and Therapy Committee of The American Academy of Periodontology and is intended for the information of the dental profession. It represents the position of the Academy regarding the current state of knowledge about treatment of gingivitis and periodontitis. The other papers are entitled The Etiology and Pathogenesis of Periodontal Diseases and Diagnosis of Periodontal Diseases.", "gemini": "Bài báo này là một trong ba bài trong một loạt bài do Ủy ban Nghiên cứu, Khoa học và Trị liệu của Học viện Nha chu Hoa Kỳ chuẩn bị và nhằm cung cấp thông tin cho ngành nha khoa. Bài viết này thể hiện quan điểm của Học viện về tình trạng kiến thức hiện tại về điều trị viêm nướu và viêm nha chu. Các bài báo khác có tựa đề Nguyên nhân và sinh bệnh học của bệnh nha chu và Chẩn đoán bệnh nha chu."} +{"idx": 1950137, "en": "A very large number of biologically active substances are released into the circulation under conditions of endotoxaemia and sepsis. One of the most important of these is nitric oxide. Under these conditions nitric oxide is produced through an induced enzyme (nitric oxide synthase) in a variety of tissues and the nitric oxide so generated is largely responsible for the loss of vascular reactivity, which occurs under these conditions, for the resulting unrelenting hypotension associated with the hypodynamic phase of septic shock. Nitric oxide also contributes to the myocardial depression in this condition. The question as to whether it is a worthwhile therapeutic approach to inhibit nitric oxide synthase is discussed with particular reference to the generation of inhibitors selective for the induced form of the enzyme. This approach has certain benefits but may also be detrimental. The fact that nitric oxide is not the key mediator involved in ultimate mortality in this condition is suggested by the failure to improve mortality in iNOS knockout mice given endotoxin.", "gemini": "Một số lượng rất lớn các chất có hoạt tính sinh học được giải phóng vào hệ tuần hoàn trong tình trạng nhiễm độc nội sinh và nhiễm trùng huyết. Một trong những chất quan trọng nhất trong số này là nitric oxide. Trong những điều kiện này, nitric oxide được sản sinh thông qua một loại enzyme được tạo ra (nitric oxide synthase) trong nhiều loại mô và nitric oxide được tạo ra như vậy phần lớn chịu trách nhiệm cho việc mất phản ứng mạch máu, xảy ra trong những điều kiện này, đối với tình trạng hạ huyết áp dai dẳng liên quan đến giai đoạn giảm động của sốc nhiễm trùng. Nitric oxide cũng góp phần gây ức chế cơ tim trong tình trạng này. Câu hỏi đặt ra là liệu có nên ức chế nitric oxide synthase như một biện pháp điều trị có giá trị hay không được thảo luận với sự tham khảo đặc biệt đến việc tạo ra các chất ức chế có chọn lọc đối với dạng enzyme được tạo ra. Cách tiếp cận này có một số lợi ích nhưng cũng có thể gây bất lợi. Thực tế là nitric oxide không phải là chất trung gian chính liên quan đến tỷ lệ tử vong cuối cùng trong tình trạng này được gợi ý bởi việc không cải thiện được tỷ lệ tử vong ở những con chuột loại trừ iNOS được dùng nội độc tố."} +{"idx": 1950138, "en": "Cytokines are an essential component in those mechanisms that depress renal function in response to inflammatory diseases of the kidney. We have addressed the role of tumor necrosis factor (TNF) in mediating changes in renal function by isolating a nephron segment, the medullary thick ascending limb of Henle's loop (mTAL), and studying the effects of TNF on ion transport via changes in the activity of two epoxygenases, cyclooxygenase (COX) and cytochrome P450 (CYP450) monooxygenase. This nephron segment generates 20-hydroxyeicosatetraenoic acid (20-HETE) as a principal arachidonate metabolite. However, when challenged with lipopolysaccharide (LPS), the mTAL expresses a considerable capacity to metabolize arachidonic acid (AA) via a COX pathway to form PGE2.", "gemini": "Cytokine là một thành phần thiết yếu trong các cơ chế làm giảm chức năng thận để đáp ứng với các bệnh viêm ở thận. Chúng tôi đã giải quyết vai trò của yếu tố hoại tử khối u (TNF) trong việc trung gian các thay đổi trong chức năng thận bằng cách phân lập một phân đoạn nephron, nhánh lên dày tủy của quai Henle (mTAL) và nghiên cứu các tác động của TNF lên quá trình vận chuyển ion thông qua các thay đổi trong hoạt động của hai epoxygenase, cyclooxygenase (COX) và cytochrome P450 (CYP450) monooxygenase. Phân đoạn nephron này tạo ra axit 20-hydroxyeicosatetraenoic (20-HETE) như một chất chuyển hóa arachidonate chính. Tuy nhiên, khi bị thách thức với lipopolysaccharide (LPS), mTAL thể hiện khả năng đáng kể trong việc chuyển hóa axit arachidonic (AA) thông qua một con đường COX để tạo thành PGE2."} +{"idx": 1950139, "en": "The available data supporting the role of oxygen radicals (OR0) as fundamental--though not unique--mediators in the pathogenesis of sepsis and septic shock are reviewed. The main questions are 1) Are there any signs of OR0 overgeneration during sepsis and septic shock? 2) Are there any indirect signs of OR0 damage during sepsis and septic shock? 3) Does OR0 inactivation affect the evolution and severity of sepsis and septic shock? 4) May the pathophysiology of sepsis and septic shock be viewed as a consequence of OR0 overgeneration? All these questions might receive affirmative answers; thus the relationships of OR0 with other mediators of sepsis and septic shock are discussed. It emerges that all the molecules which are being considered as mediators of sepsis and septic shock (cytokines, endothelins, platelet activating factor, complement) may act on granulocytes and other phagocytic cells to stimulate OR0 production. There are also very close relationships with nitric oxide, another free radical. The data in favour of an antioxidant therapy of sepsis and septic shock in humans are too few to be conclusive.", "gemini": "Dữ liệu có sẵn hỗ trợ vai trò của các gốc oxy (OR0) như các chất trung gian cơ bản - mặc dù không phải là duy nhất - trong quá trình sinh bệnh của nhiễm trùng huyết và sốc nhiễm trùng được xem xét lại. Các câu hỏi chính là 1) Có bất kỳ dấu hiệu nào của quá trình tạo ra OR0 trong quá trình nhiễm trùng huyết và sốc nhiễm trùng không? 2) Có bất kỳ dấu hiệu gián tiếp nào của tổn thương OR0 trong quá trình nhiễm trùng huyết và sốc nhiễm trùng không? 3) Việc bất hoạt OR0 có ảnh hưởng đến quá trình tiến triển và mức độ nghiêm trọng của nhiễm trùng huyết và sốc nhiễm trùng không? 4) Liệu bệnh sinh lý của nhiễm trùng huyết và sốc nhiễm trùng có thể được coi là hậu quả của quá trình tạo ra OR0 không? Tất cả những câu hỏi này đều có thể nhận được câu trả lời khẳng định; do đó, mối quan hệ của OR0 với các chất trung gian khác của nhiễm trùng huyết và sốc nhiễm trùng được thảo luận. Kết quả là tất cả các phân tử đang được coi là chất trung gian của nhiễm trùng huyết và sốc nhiễm trùng (cytokine, endothelins, yếu tố hoạt hóa tiểu cầu, bổ sung) đều có thể tác động lên bạch cầu hạt và các tế bào thực bào khác để kích thích sản xuất OR0. Ngoài ra còn có mối quan hệ rất chặt chẽ với oxit nitric, một gốc tự do khác. Dữ liệu ủng hộ liệu pháp chống oxy hóa nhiễm trùng huyết và sốc nhiễm trùng ở người còn quá ít để có thể kết luận."} +{"idx": 1950140, "en": "Leukotrienes have been identified in various pathophysiologies. The leukotrienes LTB4 and LTC4 are assigned to inflammation. 5-lipoxygenase inhibitors which inhibit the synthesis of LTA4 being the precursor of both LTB4 and LTC4 appear to have only a limited antiinflammatory potential. 5-lipoxygenase inhibitors are represented by direct and indirect inhibitors, the latter competing with substrate transfer from the five-lipoxygenase activating protein (FLAP) to the 5-lipoxygenase enzyme. 5-lipoxygenase inhibition under experimental condition results in inhibition of edema formation, neutrophil infiltration, smooth muscle contraction after antigen challenge and prevention of early and late allergic reactions. Only in the cysteinyl-leukotriene-driven pathophysiology of allergic asthma and allergic rhinitis 5-lipoxygenase inhibition appears to provide symptomatic relief. Yet, the overall-antiinflammatory effect in man in far less than expected, but may be outweighed by the nearly total lack of any side effects of 5-lipoxygenase inhibition per se.", "gemini": "Leukotrienes đã được xác định trong nhiều bệnh lý sinh lý khác nhau. Các leukotrienes LTB4 và LTC4 được chỉ định cho tình trạng viêm. Các chất ức chế 5-lipoxygenase ức chế quá trình tổng hợp LTA4 là tiền chất của cả LTB4 và LTC4 dường như chỉ có tiềm năng chống viêm hạn chế. Các chất ức chế 5-lipoxygenase được biểu thị bằng các chất ức chế trực tiếp và gián tiếp, chất sau cạnh tranh với quá trình chuyển chất nền từ protein hoạt hóa năm lipoxygenase (FLAP) sang enzyme 5-lipoxygenase. Ức chế 5-lipoxygenase trong điều kiện thực nghiệm dẫn đến ức chế sự hình thành phù nề, thâm nhiễm bạch cầu trung tính, co cơ trơn sau khi tiếp xúc với kháng nguyên và ngăn ngừa phản ứng dị ứng sớm và muộn. Chỉ trong bệnh lý sinh lý do cysteinyl-leukotriene gây ra của bệnh hen suyễn dị ứng và viêm mũi dị ứng, ức chế 5-lipoxygenase dường như mang lại hiệu quả làm giảm triệu chứng. Tuy nhiên, tác dụng chống viêm toàn thể ở người ít hơn nhiều so với dự kiến, nhưng có thể bị ảnh hưởng bởi việc gần như hoàn toàn không có bất kỳ tác dụng phụ nào của việc ức chế 5-lipoxygenase."} +{"idx": 1950141, "en": "Acute respiratory distress syndrome (ARDS) is a condition characterized by a high permeability oedema due to loss of the integrity of the alveolo-capillary barrier with impairment of normal surfactant function, resulting in an increased collapse tendency of the alveoli. Mechanical ventilation on such alveoli with repeated alveolar collapse and subsequent reexpansion results in severe lung parenchymal injury and may induce further surfactant impairment. This cam be prevented by maintaining alveolar volume at end-expiration by means of sufficient levels of positive end-expiratory pressure (PEEP). Recent evidence from experimental studies has shown that ventilator modes which allow end-expiratory collapse can induce bacterial translocation from the lung into the bloodstream and trigger the release of inflammatory mediators, which can also be presented by maintaining end-expiratory alveolar volume. These data suggest that the interaction between surfactant changes and mechanical ventilation may play a role in the transition of ARDS into the systematic inflammatory disease process of multiple system organ failure (MSOF).", "gemini": "Hội chứng suy hô hấp cấp tính (ARDS) là một tình trạng đặc trưng bởi phù thũng có tính thấm cao do mất tính toàn vẹn của hàng rào phế nang mao mạch với sự suy giảm chức năng chất hoạt động bề mặt bình thường, dẫn đến xu hướng xẹp phế nang tăng lên. Thông khí cơ học trên các phế nang như vậy với tình trạng xẹp phế nang lặp đi lặp lại và tái nở sau đó dẫn đến tổn thương nhu mô phổi nghiêm trọng và có thể gây suy giảm chất hoạt động bề mặt hơn nữa. Điều này có thể được ngăn ngừa bằng cách duy trì thể tích phế nang khi kết thúc thì thở ra bằng các mức áp lực cuối thì thở ra dương tính (PEEP) đủ. Các bằng chứng gần đây từ các nghiên cứu thực nghiệm đã chỉ ra rằng các chế độ thở máy cho phép xẹp phế nang khi kết thúc thì thở ra có thể gây ra sự dịch chuyển vi khuẩn từ phổi vào máu và kích hoạt giải phóng các chất trung gian gây viêm, cũng có thể được biểu hiện bằng cách duy trì thể tích phế nang khi kết thúc thì thở ra. Những dữ liệu này gợi ý rằng sự tương tác giữa những thay đổi của chất hoạt động bề mặt và thông khí cơ học có thể đóng vai trò trong quá trình chuyển đổi ARDS thành quá trình bệnh viêm hệ thống của suy đa cơ quan (MSOF)."} +{"idx": 1950142, "en": "Posttrauma adult respiratory distress syndrome (ARDS) and sepsis initiate complex humoral and cellular inflammatory responses that initially effect the microvascular system, but rapidly extend to involve and modulate the solid organ metabolic response. It is discussed whether the interaction between these cellular processes and the organs which they involve appear to be initiated by the trauma induced oxygen debt.", "gemini": "Hội chứng suy hô hấp cấp tính (ARDS) ở người lớn sau chấn thương và nhiễm trùng khởi phát các phản ứng viêm tế bào và dịch thể phức tạp, ban đầu ảnh hưởng đến hệ thống mạch máu nhỏ, nhưng nhanh chóng mở rộng để liên quan và điều chỉnh phản ứng trao đổi chất của cơ quan đặc. Người ta thảo luận xem liệu sự tương tác giữa các quá trình tế bào này và các cơ quan mà chúng liên quan có vẻ như được khởi phát bởi nợ oxy do chấn thương gây ra hay không."} +{"idx": 1950143, "en": "The hemodynamic consequences of both spontaneous and positive-pressure ventilation may be profound and may have opposite effects on cardiovascular stability in differing patient populations. Thus, no firm rules apply as to the specific response that will be seen in all patients and under all conditions. Some generalities, however, are probably reasonable. In patients with markedly increased work of breathing, hypervolemia, or impaired LV pump function, the institution of mechanical ventilatory support can be lifesaving because of its ability to support the cardiovascular system, independent of any beneficial effects that mechanical ventilation may have on gas exchange. In patients with decreased pulmonary elastic recoil, increased pulmonary vascular resistance, hypovolemia, or airflow obstruction, the institution of mechanical ventilatory support may induce cardiovascular instability, which, if not corrected, can lead to total cardiovascular collapse. Similarly, withdrawal of ventilatory support invariably increases intrathoracic blood volume and LV afterload and can be thought of as a type of cardiovascular stress test. Patients who pass this test easily can usually be successfully weaned from mechanical ventilatory support, whereas those who fail often are not ready to be weaned. Some patients who fail weaning trials do so because of the cardiovascular effects of spontaneous ventilation, not because the work of breathing is too great. Identification of such patients early on may improve their treatment by directing supportive therapies toward cardiovascular rather than ventilatory endpoints. However, in many situations, it will be difficult to single out a primary process determining cardiovascular instability, because multiple factors are compounded to create the observed situation and the patient's response to initiation of ventilatory support or weaning. Thus, the clinician is left with a series of therapeutic options, which if depending on the patient's response, suggest specific origins of the ventilatory and cardiovascular dysfunction. In that regard, the initiation and withdrawal of ventilatory support can be seen as a ventilatory probe into the determinants of cardiovascular homeostasis in the ventilatory-dependent patient.", "gemini": "Hậu quả về huyết động học của cả thông khí tự phát và thông khí áp lực dương có thể rất sâu sắc và có thể có tác dụng ngược lại đối với sự ổn định tim mạch ở các quần thể bệnh nhân khác nhau. Do đó, không có quy tắc chắc chắn nào áp dụng cho phản ứng cụ thể sẽ được thấy ở tất cả bệnh nhân và trong mọi điều kiện. Tuy nhiên, một số khái quát có thể là hợp lý. Ở những bệnh nhân có công thở tăng đáng kể, tăng thể tích máu hoặc chức năng bơm LV bị suy yếu, việc thiết lập hỗ trợ thông khí cơ học có thể cứu sống bệnh nhân vì khả năng hỗ trợ hệ thống tim mạch của nó, không phụ thuộc vào bất kỳ tác dụng có lợi nào mà thông khí cơ học có thể có đối với trao đổi khí. Ở những bệnh nhân giảm độ đàn hồi phổi, tăng sức cản mạch phổi, giảm thể tích máu hoặc tắc nghẽn đường thở, việc thiết lập hỗ trợ thông khí cơ học có thể gây ra tình trạng không ổn định tim mạch, nếu không được điều chỉnh, có thể dẫn đến suy tim mạch hoàn toàn. Tương tự như vậy, việc ngừng hỗ trợ thông khí luôn làm tăng thể tích máu trong lồng ngực và hậu gánh LV và có thể được coi là một loại xét nghiệm gắng sức tim mạch. Những bệnh nhân vượt qua bài kiểm tra này một cách dễ dàng thường có thể cai máy thở thành công, trong khi những bệnh nhân không vượt qua thường chưa sẵn sàng để cai máy. Một số bệnh nhân không cai máy thành công là do các tác động tim mạch của thông khí tự phát, không phải do công thở quá lớn. Việc xác định những bệnh nhân như vậy sớm có thể cải thiện việc điều trị của họ bằng cách hướng các liệu pháp hỗ trợ đến các điểm cuối về tim mạch chứ không phải thông khí. Tuy nhiên, trong nhiều tình huống, sẽ rất khó để xác định một quá trình chính xác định tình trạng không ổn định tim mạch, vì nhiều yếu tố được kết hợp để tạo ra tình trạng được quan sát thấy và phản ứng của bệnh nhân khi bắt đầu hỗ trợ thông khí hoặc cai máy. Do đó, bác sĩ lâm sàng còn lại một loạt các lựa chọn điều trị, tùy thuộc vào phản ứng của bệnh nhân, gợi ý nguồn gốc cụ thể của tình trạng rối loạn thông khí và tim mạch. Về vấn đề đó, việc bắt đầu và ngừng hỗ trợ thông khí có thể được coi là một thăm dò thông khí vào các yếu tố quyết định cân bằng nội môi tim mạch ở bệnh nhân phụ thuộc vào thông khí. Về mặt đó, việc bắt đầu và ngừng hỗ trợ thông khí có thể được coi là một thăm dò thông khí vào các yếu tố quyết định cân bằng nội môi tim mạch ở bệnh nhân phụ thuộc vào thông khí."} diff --git a/retranslated_vi_pubmed/chunk_195/error.json b/retranslated_vi_pubmed/chunk_195/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_195/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_196/completed.json b/retranslated_vi_pubmed/chunk_196/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..df9aae4b0736e33d9442620e275972a68c37dbf1 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_196/completed.json @@ -0,0 +1,146 @@ +[ + 1960000, + 1960001, + 1960002, + 1960003, + 1960004, + 1960005, + 1960006, + 1960007, + 1960008, + 1960009, + 1960010, + 1960011, + 1960012, + 1960013, + 1960014, + 1960015, + 1960016, + 1960017, + 1960018, + 1960019, + 1960020, + 1960021, + 1960022, + 1960023, + 1960024, + 1960025, + 1960026, + 1960027, + 1960028, + 1960029, + 1960030, + 1960031, + 1960032, + 1960033, + 1960034, + 1960035, + 1960036, + 1960037, + 1960038, + 1960039, + 1960040, + 1960041, + 1960042, + 1960043, + 1960044, + 1960045, + 1960046, + 1960047, + 1960048, + 1960049, + 1960050, + 1960051, + 1960052, + 1960053, + 1960054, + 1960055, + 1960056, + 1960057, + 1960058, + 1960059, + 1960060, + 1960061, + 1960062, + 1960063, + 1960064, + 1960065, + 1960066, + 1960067, + 1960068, + 1960069, + 1960070, + 1960071, + 1960072, + 1960073, + 1960074, + 1960075, + 1960076, + 1960077, + 1960078, + 1960079, + 1960080, + 1960081, + 1960082, + 1960083, + 1960084, + 1960085, + 1960086, + 1960087, + 1960088, + 1960089, + 1960090, + 1960091, + 1960092, + 1960093, + 1960094, + 1960095, + 1960096, + 1960097, + 1960098, + 1960099, + 1960100, + 1960101, + 1960102, + 1960103, + 1960104, + 1960105, + 1960106, + 1960107, + 1960108, + 1960109, + 1960110, + 1960111, + 1960112, + 1960113, + 1960114, + 1960115, + 1960116, + 1960117, + 1960118, + 1960119, + 1960120, + 1960121, + 1960122, + 1960123, + 1960124, + 1960125, + 1960126, + 1960127, + 1960128, + 1960129, + 1960130, + 1960131, + 1960132, + 1960133, + 1960134, + 1960135, + 1960136, + 1960137, + 1960138, + 1960139, + 1960140, + 1960141, + 1960142, + 1960143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_196/data.jsonl b/retranslated_vi_pubmed/chunk_196/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..80ab87e2bde49a7195a996a69ee828aeb0a20fd5 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_196/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1960000, "en": "Cytokines are hypothesized to play a central role in the pathophysiology of IgG-mediated hemolytic transfusion reactions (HTRs), and deeper understanding is required for improving therapy for these events. After establishing well-defined mouse models of HTRs, we tested whether cytokines were involved. Red blood cells (RBCs) from human glycophorin A transgenic (hGPA-Tg) or wild-type (WT) mice were transfused into non-Tg recipients passively immunized with monoclonal antibodies (Mabs). Only transfusions of incompatible RBCs induced IgG-mediated HTRs, exemplified by rapid clearance and hemoglobinuria. Very high plasma levels of monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6), and lower levels of tumor necrosis factor-alpha (TNF-alpha), were induced after incompatible transfusion. No significant changes in IL-10, IL-12, or interferon-gamma (IFN-gamma) levels were observed. The proinflammatory cytokines elaborated in this in vivo mouse model are also implicated in the systemic inflammatory response syndrome (SIRS) and confirm the hypothesis that cytokine storm occurs as a result of HTRs.", "gemini": "Người ta đưa ra giả thuyết rằng các cytokine đóng vai trò trung tâm trong sinh lý bệnh của các phản ứng truyền máu tan máu do IgG (HTR) và cần hiểu sâu hơn để cải thiện liệu pháp cho các sự kiện này. Sau khi thiết lập các mô hình chuột HTR được xác định rõ, chúng tôi đã kiểm tra xem các cytokine có liên quan hay không. Các tế bào hồng cầu (RBC) từ chuột chuyển gen glycophorin A của người (hGPA-Tg) hoặc chuột kiểu hoang dã (WT) được truyền vào những người nhận không phải Tg được miễn dịch thụ động bằng các kháng thể đơn dòng (Mabs). Chỉ truyền các RBC không tương thích mới gây ra HTR do IgG, được chứng minh bằng sự thanh thải nhanh chóng và huyết sắc niệu. Nồng độ protein hấp dẫn chemoattractant đơn bào-1 (MCP-1) và interleukin-6 (IL-6) trong huyết tương rất cao và nồng độ yếu tố hoại tử khối u-alpha (TNF-alpha) thấp hơn được gây ra sau khi truyền không tương thích. Không quan sát thấy thay đổi đáng kể nào về nồng độ IL-10, IL-12 hoặc interferon-gamma (IFN-gamma). Các cytokine gây viêm được xây dựng trong mô hình chuột in vivo này cũng có liên quan đến hội chứng đáp ứng viêm toàn thân (SIRS) và xác nhận giả thuyết rằng cơn bão cytokine xảy ra do HTR."} +{"idx": 1960001, "en": "We have recently described a new form of light chain deposition disease (LCDD) presenting as a severe cystic lung disorder requiring lung transplantation. There was no bone marrow plasma cell proliferation. Because of the absence of disease recurrence after bilateral lung transplantation and of serum-free light chain ratio normalization after the procedure, we hypothesized that monoclonal light chain synthesis occurred within the lung. The aim of this study was to look for the monoclonal B-cell component in 3 patients with cystic lung LCDD. Histologic examination of the explanted lungs showed diffuse nonamyloid kappa light chain deposits associated with a mild lymphoid infiltrate composed of aggregates of small CD20(+), CD5(-), CD10(-) B lymphocytes reminiscent of bronchus-associated lymphoid tissue. Using polymerase chain reaction (PCR), we identified a dominant B-cell clone in the lung in the 3 studied patients. The clonal expansion of each patient shared an unmutated antigen receptor variable region sequence characterized by the use of IGHV4-34 and IGKV1 subgroups with heavy and light chain CDR3 sequences of more than 80% amino acid identity, a feature evocative of an antigen-driven process. Combined with clinical and biologic data, our results strongly argue for a new antigen-driven primary pulmonary lymphoproliferative disorder.", "gemini": "Gần đây, chúng tôi đã mô tả một dạng mới của bệnh lắng đọng chuỗi nhẹ (LCDD) biểu hiện là một rối loạn phổi nang nghiêm trọng đòi hỏi phải ghép phổi. Không có sự tăng sinh tế bào plasma tủy xương. Do không có bệnh tái phát sau khi ghép phổi hai bên và tỷ lệ chuỗi nhẹ không có huyết thanh bình thường hóa sau thủ thuật, chúng tôi đưa ra giả thuyết rằng sự tổng hợp chuỗi nhẹ đơn dòng xảy ra trong phổi. Mục đích của nghiên cứu này là tìm thành phần tế bào B đơn dòng ở 3 bệnh nhân mắc LCDD phổi nang. Kiểm tra mô học của phổi đã cắt bỏ cho thấy các lắng đọng chuỗi nhẹ kappa không phải amiloid khuếch tán liên quan đến thâm nhiễm lympho nhẹ bao gồm các tập hợp tế bào lympho B nhỏ CD20 (+), CD5 (-), CD10 (-) gợi nhớ đến mô lympho liên quan đến phế quản. Sử dụng phản ứng chuỗi polymerase (PCR), chúng tôi đã xác định một dòng tế bào B trội trong phổi ở 3 bệnh nhân được nghiên cứu. Sự mở rộng dòng của mỗi bệnh nhân có chung trình tự vùng biến đổi thụ thể kháng nguyên chưa đột biến đặc trưng bởi việc sử dụng các phân nhóm IGHV4-34 và IGKV1 với các trình tự CDR3 chuỗi nặng và nhẹ có hơn 80% tính đồng nhất về axit amin, một đặc điểm gợi lên quá trình do kháng nguyên thúc đẩy. Kết hợp với dữ liệu lâm sàng và sinh học, kết quả của chúng tôi cho thấy mạnh mẽ về một rối loạn tăng sinh lympho phổi nguyên phát mới do kháng nguyên thúc đẩy."} +{"idx": 1960002, "en": "Emerging data suggest a critical role for bone marrow angiogenesis in hematologic malignancies. The angiopoietin/Tie ligand-receptor system is an essential regulator of this process. We evaluated whether circulating angiopoietin-2 (Ang-2) is a predictor for the probability of disease-free survival (DFS) in allogeneic hematopoietic stem cell transplantation (allo-HSCT) for high-risk acute myeloid leukemia or myelodysplastic syndrome. Ang-2 was measured by enzyme-linked immunosorbent assay in serum from 20 healthy controls and 90 patients with acute myeloid leukemia or myelodysplastic syndrome before conditioning for HSCT. Circulating Ang-2 was elevated in patients (median, 2.21 ng/mL; range, 0.18-48.84 ng/mL) compared with controls (median, 0.87 ng/mL; range, 0.27-4.51 ng/mL; P < .001). Multivariate analyses confirmed the independent prognostic impact of Ang-2 (hazard ratio [HR] = 2.46; 95% confidence interval [CI], 1.27-4.76, P = .005), percentage of bone marrow infiltration (HR = 1.14; 95% CI, 1.01-1.29, P = .033), and chemotherapy cycles before HSCT (HR = 1.38; 95% CI, 1.01-1.08, P = .048). Regression tree analysis detected optimal cutoff values for Ang-2 and recursively identified bone marrow blasts and Ang-2 as the best predictors for DFS. Because few predictors for DFS exist in the setting of allo-HSCT, Ang-2 may be used as a readily available powerful biomarker to pre-estimate DFS and may open new perspectives for risk-adapted treatment of high-risk myeloid malignancies.", "gemini": "Dữ liệu mới nổi cho thấy vai trò quan trọng của quá trình tạo mạch tủy xương trong các bệnh ác tính về huyết học. Hệ thống phối tử-thụ thể angiopoietin / Tie là một bộ điều chỉnh thiết yếu của quá trình này. Chúng tôi đã đánh giá xem angiopoietin-2 (Ang-2) lưu hành có phải là yếu tố dự báo khả năng sống sót không có bệnh (DFS) trong ghép tế bào gốc tạo máu đồng loại (allo-HSCT) đối với bệnh bạch cầu cấp tính nguy cơ cao hoặc hội chứng loạn sản tủy xương hay không. Ang-2 được đo bằng xét nghiệm miễn dịch liên kết enzyme trong huyết thanh của 20 người đối chứng khỏe mạnh và 90 bệnh nhân mắc bệnh bạch cầu cấp tính hoặc hội chứng loạn sản tủy xương trước khi điều trị HSCT. Ang-2 lưu hành tăng cao ở những bệnh nhân (trung bình, 2,21 ng / mL; phạm vi, 0,18-48,84 ng / mL) so với nhóm đối chứng (trung bình, 0,87 ng / mL; phạm vi, 0,27-4,51 ng / mL; P <0,001). Các phân tích đa biến xác nhận tác động tiên lượng độc lập của Ang-2 (tỷ lệ nguy cơ [HR] = 2,46; khoảng tin cậy 95% [CI], 1,27-4,76, P = 0,005), tỷ lệ thâm nhiễm tủy xương (HR = 1,14; 95% CI, 1,01-1,29, P = 0,033) và các chu kỳ hóa trị trước HSCT (HR = 1,38; 95% CI, 1,01-1,08, P = 0,048). Phân tích cây hồi quy phát hiện các giá trị ngắt tối ưu cho Ang-2 và tái phát hiện các vụ nổ tủy xương và Ang-2 là những yếu tố dự báo tốt nhất cho DFS. Vì có rất ít yếu tố dự báo DFS trong bối cảnh allo-HSCT, nên Ang-2 có thể được sử dụng như một sinh học chỉ điểm mạnh mẽ có sẵn để ước tính trước DFS và có thể mở ra những triển vọng mới cho phương pháp điều trị thích ứng với rủi ro của các bệnh ác tính tủy cấp cao."} +{"idx": 1960003, "en": "High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE (VCS)/Ge-1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.", "gemini": "Tính bổ sung cao giữa các phân tử microRNA (miRNA) của thực vật và mục tiêu RNA thông tin của chúng được cho là gây ra sự im lặng, chủ yếu thông qua quá trình phân cắt nội nhân. Chúng tôi đã phân lập được các đột biến Arabidopsis bị khiếm khuyết trong hoạt động của miRNA. Phân tích của họ cung cấp bằng chứng rằng sự im lặng được hướng dẫn bởi miRNA của thực vật có một thành phần ức chế dịch mã lan rộng có thể tách biệt về mặt di truyền khỏi quá trình phân cắt nội nhân. Chúng tôi tiếp tục chỉ ra rằng điều tương tự cũng đúng đối với sự im lặng được trung gian bởi quần thể RNA can thiệp nhỏ (siRNA). Sự ức chế dịch mã có hiệu lực một phần bởi các protein ARGONAUTE AGO1 và AGO10. Nó cũng đòi hỏi hoạt động của enzyme cắt vi ống katanin, liên quan đến động lực học của bộ xương tế bào trong hoạt động của miRNA, như được gợi ý gần đây từ các nghiên cứu trên động vật. Cũng như ở động vật, thành phần khử mũ VARICOSE (VCS)/Ge-1 là cần thiết để ức chế dịch mã bởi miRNA, điều này cho thấy các cơ chế cơ bản ở hai vương quốc có liên quan."} +{"idx": 1960004, "en": "Binary pulsar systems are superb probes of stellar and binary evolution and the physics of extreme environments. In a survey with the Arecibo telescope, we have found PSR J1903+0327, a radio pulsar with a rotational period of 2.15 milliseconds in a highly eccentric (e = 0.44) 95-day orbit around a solar mass (M(middle dot in circle)) companion. Infrared observations identify a possible main-sequence companion star. Conventional binary stellar evolution models predict neither large orbital eccentricities nor main-sequence companions around millisecond pulsars. Alternative formation scenarios involve recycling a neutron star in a globular cluster, then ejecting it into the Galactic disk, or membership in a hierarchical triple system. A relativistic analysis of timing observations of the pulsar finds its mass to be 1.74 +/- 0.04 M solar symbol, an unusually high value.", "gemini": "Các hệ nhị phân pulsar là những đầu dò tuyệt vời về sự tiến hóa của sao và nhị phân và vật lý của các môi trường khắc nghiệt. Trong một cuộc khảo sát với kính thiên văn Arecibo, chúng tôi đã tìm thấy PSR J1903+0327, một sao xung vô tuyến có chu kỳ quay là 2,15 mili giây trong quỹ đạo 95 ngày có độ lệch tâm cao (e = 0,44) xung quanh một ngôi sao đồng hành có khối lượng mặt trời (M(dấu chấm giữa trong vòng tròn)). Các quan sát hồng ngoại xác định một ngôi sao đồng hành có thể là sao dãy chính. Các mô hình tiến hóa sao nhị phân thông thường không dự đoán độ lệch tâm quỹ đạo lớn cũng như các ngôi sao đồng hành dãy chính xung quanh các sao xung mili giây. Các kịch bản hình thành thay thế liên quan đến việc tái chế một ngôi sao neutron trong một cụm cầu, sau đó đẩy nó vào đĩa Ngân hà hoặc thành viên trong một hệ ba phân cấp. Phân tích tương đối luận về các quan sát thời gian của sao xung cho thấy khối lượng của nó là 1,74 +/- 0,04 M biểu tượng mặt trời, một giá trị cao bất thường."} +{"idx": 1960005, "en": "The Shallow Radar (SHARAD) on the Mars Reconnaissance Orbiter has imaged the internal stratigraphy of the north polar layered deposits of Mars. Radar reflections within the deposits reveal a laterally continuous deposition of layers, which typically consist of four packets of finely spaced reflectors separated by homogeneous interpacket regions of nearly pure ice. The packet/interpacket structure can be explained by approximately million-year periodicities in Mars' obliquity or orbital eccentricity. The observed approximately 100-meter maximum deflection of the underlying substrate in response to the ice load implies that the present-day thickness of an equilibrium elastic lithosphere is greater than 300 kilometers. Alternatively, the response to the load may be in a transient state controlled by mantle viscosity. Both scenarios probably require that Mars has a subchondritic abundance of heat-producing elements.", "gemini": "Radar nông (SHARAD) trên tàu quỹ đạo trinh sát sao Hỏa đã chụp ảnh địa tầng bên trong của các lớp trầm tích phân lớp cực bắc của sao Hỏa. Các phản xạ radar bên trong các lớp trầm tích cho thấy sự lắng đọng theo chiều ngang của các lớp, thường bao gồm bốn gói các phản xạ cách đều nhau được phân tách bởi các vùng giữa các gói đồng nhất gần như là băng tinh khiết. Cấu trúc gói/giữa các gói có thể được giải thích bằng các chu kỳ khoảng một triệu năm trong độ nghiêng hoặc độ lệch tâm quỹ đạo của sao Hỏa. Độ lệch tối đa khoảng 100 mét được quan sát thấy của lớp nền bên dưới để phản ứng với tải trọng băng cho thấy rằng độ dày hiện tại của thạch quyển đàn hồi cân bằng lớn hơn 300 km. Mặt khác, phản ứng với tải trọng có thể ở trạng thái tạm thời được kiểm soát bởi độ nhớt của lớp phủ. Cả hai kịch bản có thể đều yêu cầu sao Hỏa có hàm lượng các nguyên tố sinh nhiệt dưới mức chondrit."} +{"idx": 1960006, "en": "The cellular DNA damage response (DDR) is initiated by the rapid recruitment of repair factors to the site of DNA damage to form a multiprotein repair complex. How the repair complex senses damaged DNA and then activates the DDR is not well understood. We show that prolonged binding of DNA repair factors to chromatin can elicit the DDR in an ATM (ataxia telangiectasia mutated)- and DNAPK (DNA-dependent protein kinase)-dependent manner in the absence of DNA damage. Targeting of single repair factors to chromatin revealed a hierarchy of protein interactions within the repair complex and suggests amplification of the damage signal. We conclude that activation of the DDR does not require DNA damage and stable association of repair factors with chromatin is likely a critical step in triggering, amplifying, and maintaining the DDR signal.", "gemini": "Phản ứng tổn thương DNA tế bào (DDR) được khởi phát bởi sự tuyển dụng nhanh chóng các yếu tố sửa chữa đến vị trí tổn thương DNA để tạo thành phức hợp sửa chữa đa protein. Cách thức phức hợp sửa chữa cảm nhận DNA bị hư hỏng và sau đó kích hoạt DDR vẫn chưa được hiểu rõ. Chúng tôi chỉ ra rằng sự liên kết kéo dài của các yếu tố sửa chữa DNA với chromatin có thể gây ra DDR theo cách phụ thuộc ATM (rối loạn giãn mạch máu não) và DNAPK (kinase protein phụ thuộc DNA) khi không có tổn thương DNA. Việc nhắm mục tiêu các yếu tố sửa chữa đơn lẻ vào chromatin đã tiết lộ một hệ thống phân cấp các tương tác protein trong phức hợp sửa chữa và cho thấy sự khuếch đại tín hiệu tổn thương. Chúng tôi kết luận rằng việc kích hoạt DDR không yêu cầu DNA bị hư hỏng và sự liên kết ổn định của các yếu tố sửa chữa với chromatin có khả năng là một bước quan trọng trong việc kích hoạt, khuếch đại và duy trì tín hiệu DDR."} +{"idx": 1960007, "en": "OBJECTIVE Pathological angiogenesis is an integral component of many diseases. Antiangiogenesis and vascular targeting are therefore promising new therapeutic principles. However, few endothelial-specific putative drug targets have been identified, and information is still limited about endothelial-specific molecular processes. Here we aimed at determining the endothelial cell-specific core transcriptome in vivo. METHODS AND RESULTS Analysis of publicly available microarray data identified a mixed vascular/lung cluster of 132 genes that correlated with known endothelial markers. Filtering against kidney glomerular/nonglomerular and brain vascular/nonvascular microarray profiles separated contaminating lung markers, leaving 58 genes with broad and specific microvascular expression. More than half of these have not previously been linked to endothelial functions or studied in detail before. The endothelial cell-specific expression of a selected subset of these, Eltd1, Gpr116, Ramp2, Slc9a3r2, Slc43a3, Rasip1, and NM_023516, was confirmed by real-time quantitative polymerase chain reaction and/or immunohistochemistry. CONCLUSIONS We have used a combination of publicly available and own microarray data to identify 58 gene transcripts with broad yet specific expression in microvascular endothelium. Most of these have unknown functions, but many of them are predicted to be cell surface expressed or implicated in cell signaling processes and should therefore be explored as putative microvascular drug targets.", "gemini": "MỤC TIÊU Sự hình thành mạch máu bệnh lý là một thành phần không thể thiếu của nhiều bệnh. Do đó, chống hình thành mạch máu và nhắm mục tiêu mạch máu là những nguyên tắc điều trị mới đầy hứa hẹn. Tuy nhiên, rất ít mục tiêu thuốc tiềm tàng đặc hiệu đối với nội mạc được xác định và thông tin về các quá trình phân tử đặc hiệu đối với nội mạc vẫn còn hạn chế. Ở đây, chúng tôi nhằm mục đích xác định phiên mã cốt lõi đặc hiệu của tế bào nội mô trong cơ thể sống. PHƯƠNG PHÁP VÀ KẾT QUẢ Phân tích dữ liệu microarray có sẵn công khai cho thấy một nhóm mạch máu/phổi hỗn hợp gồm 132 gen có tương quan với các dấu ấn nội mô đã biết. Việc lọc so với các cấu hình microarray cầu thận/không cầu thận và mạch não/không mạch máu đã tách các dấu ấn phổi bị nhiễm, để lại 58 gen có biểu hiện vi mạch rộng và đặc hiệu. Hơn một nửa trong số này trước đây chưa từng được liên kết với các chức năng nội mô hoặc được nghiên cứu chi tiết trước đây. Biểu hiện đặc hiệu của tế bào nội mô của một tập hợp con được chọn trong số các gen này, Eltd1, Gpr116, Ramp2, Slc9a3r2, Slc43a3, Rasip1 và NM_023516, đã được xác nhận bằng phản ứng chuỗi polymerase định lượng thời gian thực và/hoặc miễn dịch mô hóa học. KẾT LUẬN Chúng tôi đã sử dụng kết hợp dữ liệu microarray có sẵn công khai và của riêng chúng tôi để xác định 58 bản phiên mã gen có biểu hiện rộng nhưng đặc hiệu trong nội mô vi mạch. Hầu hết trong số này có chức năng chưa biết, nhưng nhiều chức năng trong số chúng được dự đoán là được biểu hiện trên bề mặt tế bào hoặc liên quan đến các quá trình truyền tín hiệu tế bào và do đó nên được khám phá như các mục tiêu thuốc vi mạch tiềm tàng."} +{"idx": 1960008, "en": "The oocyte-independent source for the generation of pluripotent stem cells is among the ultimate goals in regenerative medicine. We report that on exposure to mouse embryonic stem cell (mESC) extracts, reversibly permeabilized NIH3T3 cells undergo dedifferentiation followed by stimulus-induced redifferentiation into multiple lineage cell types. Genome-wide expression profiling revealed significant differences between NIH3T3 control and ESC extract-treated NIH3T3 cells including the reactivation of ESC-specific transcripts. Epigenetically, ESC extracts induced CpG demethylation of Oct4 promoter, hyperacetylation of histones 3 and 4, and decreased lysine 9 (K-9) dimethylation of histone 3. In mouse models of surgically induced hindlimb ischemia or acute myocardial infarction transplantation of reprogrammed NIH3T3 cells significantly improved postinjury physiological functions and showed anatomic evidence of engraftment and transdifferentiation into skeletal muscle, endothelial cell, and cardiomyocytes. These data provide evidence for the generation of functional multipotent stem-like cells from terminally differentiated somatic cells without the introduction of retroviral mediated transgenes or ESC fusion.", "gemini": "Nguồn không phụ thuộc vào tế bào trứng để tạo ra các tế bào gốc đa năng là một trong những mục tiêu cuối cùng trong y học tái tạo. Chúng tôi báo cáo rằng khi tiếp xúc với chiết xuất tế bào gốc phôi chuột (mESC), các tế bào NIH3T3 được thấm qua một cách thuận nghịch sẽ trải qua quá trình khử biệt hóa sau đó là tái biệt hóa do kích thích thành nhiều loại tế bào dòng. Hồ sơ biểu hiện trên toàn bộ hệ gen cho thấy sự khác biệt đáng kể giữa tế bào NIH3T3 đối chứng và tế bào NIH3T3 được xử lý bằng chiết xuất ESC bao gồm việc tái hoạt hóa các bản phiên mã đặc hiệu ESC. Về mặt biểu sinh, chiết xuất ESC gây ra quá trình khử methyl hóa CpG của chất xúc tác Oct4, acetyl hóa quá mức của histone 3 và 4 và làm giảm dimethyl hóa lysine 9 (K-9) của histone 3. Trong các mô hình chuột bị thiếu máu chi sau do phẫu thuật hoặc nhồi máu cơ tim cấp tính, việc cấy ghép các tế bào NIH3T3 được lập trình lại đã cải thiện đáng kể các chức năng sinh lý sau chấn thương và cho thấy bằng chứng giải phẫu về sự ghép và chuyển hóa thành cơ xương, tế bào nội mô và tế bào cơ tim. Những dữ liệu này cung cấp bằng chứng cho thấy sự tạo ra các tế bào gốc đa năng chức năng từ các tế bào soma biệt hóa cuối cùng mà không cần đưa vào các transgen do retrovirus trung gian hoặc hợp nhất ESC."} +{"idx": 1960009, "en": "The metal binding protein metallothionein (MT) is a target for nitric oxide (NO), causing release of bound zinc that affects myogenic reflex in systemic resistance vessels. Here, we investigate a role for NO-induced zinc release in pulmonary vasoregulation. We show that acute hypoxia causes reversible constriction of intraacinar arteries (<50 microm/L) in isolated perfused mouse lung (IPL). We further demonstrate that isolated pulmonary (but not aortic) endothelial cells constrict in hypoxia. Hypoxia also causes NO-dependent increases in labile zinc in mouse lung endothelial cells and endothelium of IPL. The latter observation is dependent on MT because it is not apparent in IPL of MT(-/-) mice. Data from NO-sensitive fluorescence resonance energy transfer-based reporters support hypoxia-induced NO production in pulmonary endothelium. Furthermore, hypoxic constriction is blunted in IPL of MT(-/-) mice and in wild-type mice, or rats, treated with the zinc chelator N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN), suggesting a role for chelatable zinc in modulating HPV. Finally, the NO donor DETAnonoate causes further vasoconstriction in hypoxic IPL in which NO vasodilatory pathways are inhibited. Collectively, these data suggest that zinc thiolate signaling is a component of the effects of acute hypoxia-mediated NO biosynthesis and that this pathway may contribute to constriction in the pulmonary vasculature.", "gemini": "Protein liên kết kim loại metallothionein (MT) là một mục tiêu của oxit nitric (NO), gây ra sự giải phóng kẽm liên kết ảnh hưởng đến phản xạ cơ ở các mạch máu ngoại biên. Ở đây, chúng tôi nghiên cứu vai trò của việc giải phóng kẽm do NO gây ra trong quá trình điều hòa mạch máu phổi. Chúng tôi chỉ ra rằng tình trạng thiếu oxy cấp tính gây co thắt có hồi phục các động mạch trong tiểu phế quản (<50 microm/L) trong phổi chuột được tưới máu riêng biệt (IPL). Hơn nữa, chúng tôi chứng minh rằng các tế bào nội mô phổi riêng biệt (nhưng không phải tế bào nội mô động mạch chủ) co lại trong tình trạng thiếu oxy. Tình trạng thiếu oxy cũng gây ra sự gia tăng kẽm không ổn định phụ thuộc vào NO trong các tế bào nội mô phổi chuột và nội mô của IPL. Quan sát sau phụ thuộc vào MT vì nó không xuất hiện ở IPL của chuột MT(-/-). Dữ liệu từ các chất báo cáo dựa trên sự truyền năng lượng cộng hưởng huỳnh quang nhạy cảm với NO hỗ trợ quá trình sản xuất NO do thiếu oxy trong nội mô phổi. Hơn nữa, sự co thắt do thiếu oxy bị giảm trong IPL của chuột MT(-/-) và ở chuột hoặc chuột cống hoang dã được điều trị bằng chất tạo phức kẽm N,N,N',N'-tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN), cho thấy vai trò của kẽm có thể tạo phức trong việc điều chế HPV. Cuối cùng, chất cho NO DETAnonoate gây ra sự co mạch hơn nữa ở IPL thiếu oxy trong đó các con đường giãn mạch NO bị ức chế. Nhìn chung, những dữ liệu này cho thấy tín hiệu kẽm thiolate là một thành phần của các tác động của quá trình sinh tổng hợp NO do thiếu oxy cấp tính và con đường này có thể góp phần vào sự co thắt ở hệ mạch phổi."} +{"idx": 1960010, "en": "Foam cells are a hallmark of atherosclerosis. However, it is unclear whether foam cell formation per se protects against atherosclerosis or fuels it. In this study, we investigated the role of adipose differentiation-related protein (ADFP), a major lipid droplet protein (LDP), in the regulation of foam cell formation and atherosclerosis. We show that ADFP expression facilitates foam cell formation induced by modified lipoproteins in mouse macrophages in vitro. We show further that Adfp gene inactivation in apolipoprotein E-deficient (ApoE(-/-)) mice reduces the number of lipid droplets in foam cells in atherosclerotic lesions and protects the mice against atherosclerosis. Moreover, transplantation of ADFP-null bone marrow-derived cells effectively attenuated atherosclerosis in ApoE(-/-) mice. Deficiency of ADFP did not cause a detectable compensatory increase in the other PAT domain proteins in macrophages in vitro or in vivo. Mechanistically, ADFP enables the macrophage to maintain its lipid content by hindering lipid efflux. We detected no significant difference in lesion composition or in multiple parameters of inflammation in macrophages or in their phagocytic activity between mice with and without ADFP. In conclusion, Adfp inactivation in ApoE(-/-) background protects against atherosclerosis and appears to be a relatively pure model of impaired foam cell formation.", "gemini": "Các tế bào bọt là đặc điểm của bệnh xơ vữa động mạch. Tuy nhiên, vẫn chưa rõ liệu sự hình thành tế bào bọt có tự bảo vệ chống lại bệnh xơ vữa động mạch hay thúc đẩy bệnh này. Trong nghiên cứu này, chúng tôi đã nghiên cứu vai trò của protein liên quan đến sự biệt hóa của mô mỡ (ADFP), một protein giọt lipid chính (LDP), trong quá trình điều hòa sự hình thành tế bào bọt và bệnh xơ vữa động mạch. Chúng tôi chỉ ra rằng sự biểu hiện của ADFP tạo điều kiện thuận lợi cho sự hình thành tế bào bọt do lipoprotein biến đổi gây ra ở các đại thực bào chuột trong ống nghiệm. Chúng tôi chỉ ra thêm rằng việc bất hoạt gen Adfp ở những con chuột bị thiếu apolipoprotein E (ApoE(-/-)) làm giảm số lượng giọt lipid trong các tế bào bọt ở các tổn thương xơ vữa động mạch và bảo vệ những con chuột này khỏi bệnh xơ vữa động mạch. Hơn nữa, việc cấy ghép các tế bào có nguồn gốc từ tủy xương không có ADFP làm giảm hiệu quả bệnh xơ vữa động mạch ở chuột ApoE(-/-). Sự thiếu hụt ADFP không gây ra sự gia tăng bù đáng kể các protein miền PAT khác trong các đại thực bào trong ống nghiệm hoặc trong cơ thể sống. Về mặt cơ chế, ADFP giúp đại thực bào duy trì hàm lượng lipid của nó bằng cách cản trở dòng chảy lipid. Chúng tôi không phát hiện thấy sự khác biệt đáng kể về thành phần tổn thương hoặc trong nhiều thông số về tình trạng viêm ở các đại thực bào hoặc trong hoạt động thực bào của chúng giữa những con chuột có và không có ADFP. Tóm lại, việc bất hoạt Adfp trong nền ApoE(-/-) bảo vệ chống lại bệnh xơ vữa động mạch và dường như là một mô hình tương đối thuần túy về sự hình thành tế bào bọt bị suy yếu."} +{"idx": 1960011, "en": "Notch signaling is critically important for proper architecture of the vascular system, and mutations in NOTCH3 are associated with CADASIL, a stroke and dementia syndrome with vascular smooth muscle cell (VSMC) dysfunction. In this report, we link Notch signaling to platelet-derived growth factor (PDGF) signaling, a key determinant of VSMC biology, and show that PDGF receptor (PDGFR)-beta is a novel immediate Notch target gene. PDGFR-beta expression was upregulated by Notch ligand induction or by activated forms of the Notch receptor. Moreover, upregulation of PDGFR-beta expression in response to Notch activation critically required the Notch signal integrator CSL. In primary VSMCs, PDGFR-beta expression was robustly upregulated by Notch signaling, leading to an augmented intracellular response to PDGF stimulation. In newborn Notch3-deficient mice, PDGFR-beta expression was strongly reduced in the VSMCs that later develop an aberrant morphology. In keeping with this, PDGFR-beta upregulation in response to Notch activation was reduced also in Notch3-deficient embryonic stem cells. Finally, in VSMCs from a CADASIL patient carrying a NOTCH3 missense mutation, upregulation of PDGFR-beta mRNA and protein in response to ligand-induced Notch activation was significantly reduced. In sum, these data reveal a hierarchy for 2 important signaling systems, Notch and PDGF, in the vasculature and provide insights into how dysregulated Notch signaling perturbs VSMC differentiation and function.", "gemini": "Tín hiệu Notch cực kỳ quan trọng đối với cấu trúc thích hợp của hệ thống mạch máu và các đột biến ở NOTCH3 có liên quan đến CADASIL, một hội chứng đột quỵ và mất trí nhớ do rối loạn chức năng tế bào cơ trơn mạch máu (VSMC). Trong báo cáo này, chúng tôi liên kết tín hiệu Notch với tín hiệu yếu tố tăng trưởng có nguồn gốc từ tiểu cầu (PDGF), một yếu tố quyết định chính của sinh học VSMC và chỉ ra rằng thụ thể PDGF (PDGFR)-beta là một gen đích Notch tức thời mới. Sự biểu hiện của PDGFR-beta được điều chỉnh tăng lên do sự cảm ứng của phối tử Notch hoặc bởi các dạng hoạt hóa của thụ thể Notch. Hơn nữa, sự điều chỉnh tăng lên của biểu hiện PDGFR-beta để đáp ứng với sự hoạt hóa của Notch đòi hỏi rất cần chất tích hợp tín hiệu Notch CSL. Trong VSMC sơ cấp, biểu hiện PDGFR-beta được điều chỉnh tăng lên mạnh mẽ bởi tín hiệu Notch, dẫn đến phản ứng nội bào tăng lên đối với kích thích PDGF. Ở chuột thiếu Notch3 mới sinh, biểu hiện PDGFR-beta bị giảm mạnh ở các VSMC sau này phát triển hình thái bất thường. Phù hợp với điều này, sự điều chỉnh tăng lên của PDGFR-beta để đáp ứng với sự hoạt hóa của Notch cũng bị giảm ở các tế bào gốc phôi thiếu Notch3. Cuối cùng, ở VSMC từ một bệnh nhân CADASIL mang đột biến sai nghĩa NOTCH3, sự điều chỉnh tăng lên của mRNA và protein PDGFR-beta để đáp ứng với sự hoạt hóa Notch do phối tử gây ra đã giảm đáng kể. Tóm lại, những dữ liệu này tiết lộ một hệ thống phân cấp cho 2 hệ thống tín hiệu quan trọng, Notch và PDGF, trong hệ mạch máu và cung cấp thông tin chi tiết về cách tín hiệu Notch bị rối loạn làm nhiễu loạn sự biệt hóa và chức năng của VSMC."} +{"idx": 1960012, "en": "Phenotypic switching of smooth muscle cells (SMCs) plays a key role in vascular proliferative diseases. We previously showed that Krüppel-like factor 4 (Klf4) suppressed SMC differentiation markers in cultured SMCs. Here, we derive mice deficient for Klf4 by conditional gene ablation and analyze their vascular phenotype following carotid injury. Klf4 expression was rapidly induced in SMCs of control mice after vascular injury but not in Klf4-deficient mice. Injury-induced repression of SMC differentiation markers was transiently delayed in Klf4-deficient mice. Klf4 mutant mice exhibited enhanced neointimal formation in response to vascular injury caused by increased cellular proliferation in the media but not an altered apoptotic rate. Consistent with these findings, cultured SMCs overexpressing Klf4 showed reduced cellular proliferation, in part, through the induction of the cell cycle inhibitor, p21(WAF1/Cip1) via increased binding of Klf4 and p53 to the p21(WAF1/Cip1) promoter/enhancer. In vivo chromatin immunoprecipitation assays also showed increased Klf4 binding to the promoter/enhancer regions of the p21(WAF1/Cip1) gene and SMC differentiation marker genes following vascular injury. Taken together, we have demonstrated that Klf4 plays a critical role in regulating expression of SMC differentiation markers and proliferation of SMCs in vivo in response to vascular injury.", "gemini": "Sự chuyển đổi kiểu hình của các tế bào cơ trơn (SMC) đóng vai trò chính trong các bệnh tăng sinh mạch. Trước đây chúng tôi đã chỉ ra rằng yếu tố giống Krüppel-4 (Klf4) ức chế các dấu hiệu phân biệt SMC trong các SMC nuôi cấy. Ở đây, chúng tôi tạo ra những con chuột thiếu Klf4 bằng cách loại bỏ gen có điều kiện và phân tích kiểu hình mạch máu của chúng sau chấn thương động mạch cảnh. Biểu hiện Klf4 được tạo ra nhanh chóng ở các SMC của chuột đối chứng sau chấn thương mạch máu nhưng không phải ở chuột thiếu Klf4. Sự ức chế do chấn thương gây ra đối với các dấu hiệu phân biệt SMC bị chậm lại tạm thời ở những con chuột thiếu Klf4. Chuột đột biến Klf4 biểu hiện sự hình thành tân nội mạc tăng cường đáp ứng với chấn thương mạch máu do tăng sinh tế bào ở lớp giữa nhưng không làm thay đổi tỷ lệ chết theo chương trình. Phù hợp với những phát hiện này, các SMC nuôi cấy biểu hiện quá mức Klf4 cho thấy sự tăng sinh tế bào giảm, một phần thông qua sự tạo ra chất ức chế chu kỳ tế bào, p21(WAF1/Cip1) thông qua sự liên kết tăng lên của Klf4 và p53 với chất thúc đẩy/tăng cường p21(WAF1/Cip1). Các xét nghiệm kết tủa miễn dịch chromatin trong ống nghiệm cũng cho thấy sự liên kết Klf4 tăng lên với các vùng thúc đẩy/tăng cường của gen p21(WAF1/Cip1) và các gen đánh dấu phân biệt SMC sau chấn thương mạch máu. Tổng hợp lại, chúng tôi đã chứng minh rằng Klf4 đóng vai trò quan trọng trong việc điều chỉnh biểu hiện của các dấu hiệu phân biệt SMC và sự tăng sinh của SMC trong ống nghiệm để đáp ứng với chấn thương mạch máu."} +{"idx": 1960013, "en": "BACKGROUND AND PURPOSE Falls are an important issue in older people. We aimed to determine the incidence, circumstances, and predictors of falls in patients with recent acute stroke. METHODS The Auckland Regional Community Stroke (ARCOS) study was a prospective population-based stroke incidence study conducted in Auckland, New Zealand (NZ) during 2002 to 2003. Among 6-month survivors, the location and consequences of any falls were ascertained by self-report as part of a structured interview. Multivariable logistic regression was used to establish associations between risk factors and \"any\" and \"injurious\" falls. RESULTS Of 1104 stroke survivors who completed an interview, 407 (37%) reported at least 1 fall, 151 (37% of fallers, 14% of stroke survivors) sustained an injury that required medical treatment, and 31 (8% of fallers, 3% of stroke survivors) sustained a fracture. The majority of falls occurred indoors at home. Independent factors associated with falls were depressive symptoms, disability, previous falls, and older age. For injurious falls, the positively associated factors were female sex and NZ/European ethnicity and dependence before the stroke, whereas higher levels of activity and normal cognition were negatively associated factors. CONCLUSIONS Falls are common after stroke, and their predictive factors are similar to those for older people in general. Falls prevention programs require implementation in stroke services.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Ngã là một vấn đề quan trọng ở người cao tuổi. Chúng tôi muốn xác định tỷ lệ mắc, hoàn cảnh và các yếu tố dự báo ngã ở những bệnh nhân mới bị đột quỵ cấp. PHƯƠNG PHÁP Nghiên cứu Đột quỵ cộng đồng khu vực Auckland (ARCOS) là một nghiên cứu về tỷ lệ mắc đột quỵ dựa trên dân số được tiến hành tại Auckland, New Zealand (NZ) trong giai đoạn 2002 đến 2003. Trong số những người sống sót sau 6 tháng, vị trí và hậu quả của bất kỳ lần ngã nào đều được xác định bằng cách tự báo cáo như một phần của cuộc phỏng vấn có cấu trúc. Phân tích hồi quy logistic đa biến được sử dụng để thiết lập mối liên hệ giữa các yếu tố rủi ro và \"bất kỳ\" và \"gây thương tích\" lần ngã. KẾT QUẢ Trong số 1104 người sống sót sau đột quỵ đã hoàn thành cuộc phỏng vấn, 407 (37%) báo cáo ít nhất 1 lần ngã, 151 (37% người ngã, 14% người sống sót sau đột quỵ) bị thương tích cần điều trị y tế và 31 (8% người ngã, 3% người sống sót sau đột quỵ) bị gãy xương. Hầu hết các trường hợp ngã xảy ra trong nhà. Các yếu tố độc lập liên quan đến ngã là các triệu chứng trầm cảm, khuyết tật, ngã trước đó và tuổi cao. Đối với những lần ngã gây thương tích, các yếu tố liên quan tích cực là giới tính nữ và dân tộc NZ/châu Âu và phụ thuộc trước khi đột quỵ, trong khi mức độ hoạt động cao hơn và nhận thức bình thường là các yếu tố liên quan tiêu cực. KẾT LUẬN Ngã là phổ biến sau đột quỵ và các yếu tố dự báo của chúng tương tự như đối với người cao tuổi nói chung. Các chương trình phòng ngừa ngã cần được triển khai trong các dịch vụ đột quỵ."} +{"idx": 1960014, "en": "BACKGROUND AND PURPOSE Gradient recalled echo MRI (GRE) has been shown to be as accurate as CT for the detection of acute intracerebral hemorrhage (ICH). However, because of the differences in the signal parameter being detected, apparent hemorrhage size is expected to vary by imaging modality, with GRE providing larger volumes attributable to susceptibility effects. METHODS Image data from patients participating in 3 ICH studies were retrospectively reviewed. Patients with acute ICH were included if (1) concurrent MRI and CT were performed within 72 hours of symptom onset, and (2) each modality was performed within 240 minutes of each other. ICH volumes were calculated using a semiautomated image analysis program. The least squares method was used to develop a conversion equation based on a linear regression of GRE volume on CT volume. RESULTS Thirty-six patients met inclusion criteria. MRI was performed first in 18, CT first in 18. Mean hemorrhage volume was 25.2 cc (range 0.1 to 83.9 cc) on CT and 32.7 cc (range 0.1 to 98.7 cc) measured on GRE. A linear relationship defined by CT Volume=GRE Volume*0.8 (Spearman's correlation coefficient=0.992, P<0.001) was derived. CONCLUSIONS Acute ICH volumes as measured on GRE pulse sequences are consistently larger than CT volumes. A simple mathematical conversion model has been developed: CT volume=0.8*GRE volume. This formula can be used in studies using both imaging modalities, across different studies, or to track ICH growth over time independent of imaging modality in an individual patient.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Đã chứng minh rằng MRI hồi âm gradient (GRE) chính xác như CT trong việc phát hiện xuất huyết não trong (ICH) cấp tính. Tuy nhiên, do sự khác biệt về thông số tín hiệu được phát hiện, kích thước xuất huyết biểu kiến được dự kiến sẽ thay đổi theo phương thức hình ảnh, với GRE cung cấp khối lượng lớn hơn do các hiệu ứng nhạy cảm. PHƯƠNG PHÁP Dữ liệu hình ảnh từ những bệnh nhân tham gia 3 nghiên cứu ICH đã được xem xét lại một cách hồi cứu. Những bệnh nhân bị ICH cấp tính được đưa vào nếu (1) MRI và CT đồng thời được thực hiện trong vòng 72 giờ sau khi khởi phát triệu chứng và (2) mỗi phương thức được thực hiện trong vòng 240 phút sau mỗi phương thức. Thể tích ICH được tính bằng chương trình phân tích hình ảnh bán tự động. Phương pháp bình phương nhỏ nhất được sử dụng để phát triển một phương trình chuyển đổi dựa trên hồi quy tuyến tính của thể tích GRE trên thể tích CT. KẾT QUẢ Ba mươi sáu bệnh nhân đáp ứng các tiêu chuẩn đưa vào. MRI được thực hiện trước ở 18 bệnh nhân, CT được thực hiện trước ở 18 bệnh nhân. Thể tích xuất huyết trung bình là 25,2 cc (phạm vi từ 0,1 đến 83,9 cc) trên CT và 32,7 cc (phạm vi từ 0,1 đến 98,7 cc) được đo trên GRE. Một mối quan hệ tuyến tính được xác định bởi Thể tích CT = Thể tích GRE * 0,8 (hệ số tương quan của Spearman = 0,992, P < 0,001) đã được phát hiện. KẾT LUẬN Thể tích ICH cấp tính được đo trên các chuỗi xung GRE luôn lớn hơn thể tích CT. Một mô hình chuyển đổi toán học đơn giản đã được phát triển: Thể tích CT = 0,8 * Thể tích GRE. Công thức này có thể được sử dụng trong các nghiên cứu sử dụng cả hai phương thức hình ảnh, trên các nghiên cứu khác nhau hoặc để theo dõi sự phát triển của ICH theo thời gian độc lập với phương thức hình ảnh ở một bệnh nhân cụ thể."} +{"idx": 1960015, "en": "Although platelets induce lung inflammation, leading to acute lung injury (ALI), the extent of platelet-endothelial cell (EC) interactions remains poorly understood. Here, in a ventilation-stress model of lung inflammation, we show that platelet-EC interactions are important. We obtained freshly isolated lung endothelial cells (FLECs) from isolated, blood-perfused rat lungs exposed to ventilation at low tidal volume (LV) or stress-inducing high tidal volume (HV). Immunofluorescence and immunoprecipitation studies revealed HV-induced increases in cell-surface von Willebrand factor (vWf) expression on FLEC. This increased expression was inhibited by platelet removal from the lung perfusion and by including a P-selectin-blocking antibody in the lung perfusion. The expression was also blocked in lungs from P-selectin knockout (P sel(-/-)) mice perfused with autologous blood, but not with heterologous wild-type blood containing P-selectin-expressing platelets. These findings indicate that in ventilation stress, platelets transfer vWf to the EC surface and that platelet P-selectin plays a critical role in this transfer. Further evidence for such intercellular transfers was the HV-induced FLEC expressions of platelet glycoprotein 1b and of platelet P-selectin. We conclude that in ventilation stress, platelets deposit leukocyte- and platelet-binding proteins on the EC surface, thereby establishing the proinflammatory phenotype of the vascular lining.", "gemini": "Mặc dù tiểu cầu gây viêm phổi, dẫn đến tổn thương phổi cấp tính (ALI), nhưng mức độ tương tác giữa tiểu cầu và tế bào nội mô (EC) vẫn chưa được hiểu rõ. Ở đây, trong mô hình căng thẳng thông khí gây viêm phổi, chúng tôi chỉ ra rằng các tương tác tiểu cầu-EC rất quan trọng. Chúng tôi đã thu được các tế bào nội mô phổi phân lập tươi (FLEC) từ phổi chuột được tưới máu và phân lập, tiếp xúc với thông khí ở thể tích khí lưu thông thấp (LV) hoặc thể tích khí lưu thông cao gây căng thẳng (HV). Các nghiên cứu miễn dịch huỳnh quang và kết tủa miễn dịch cho thấy HV làm tăng biểu hiện của yếu tố von Willebrand (vWf) trên bề mặt tế bào trên FLEC. Sự gia tăng biểu hiện này bị ức chế bởi việc loại bỏ tiểu cầu khỏi quá trình tưới máu phổi và bằng cách đưa vào kháng thể chẹn P-selectin trong quá trình tưới máu phổi. Biểu hiện này cũng bị chặn ở phổi của chuột loại bỏ gen P-selectin (P sel(-/-)) được tưới máu bằng máu tự thân, nhưng không phải bằng máu đồng loại có chứa tiểu cầu biểu hiện P-selectin. Những phát hiện này chỉ ra rằng trong căng thẳng thông khí, tiểu cầu chuyển vWf đến bề mặt EC và P-selectin của tiểu cầu đóng vai trò quan trọng trong quá trình chuyển này. Một bằng chứng khác cho sự chuyển giao giữa các tế bào như vậy là biểu hiện glycoprotein 1b của tiểu cầu và P-selectin của tiểu cầu do HV gây ra trên FLEC. Chúng tôi kết luận rằng trong căng thẳng thông khí, tiểu cầu lắng đọng các protein liên kết tiểu cầu và bạch cầu trên bề mặt EC, do đó tạo ra kiểu hình tiền viêm của lớp lót mạch máu."} +{"idx": 1960016, "en": "Streptococcus pneumoniae is the leading pathogen of community-acquired pneumonia and is a main cause of infectious deaths. However, little is known about host-pathogen interaction in human lung tissue. We tested the hypothesis that human alveolar macrophages (AMs) and alveolar epithelial cells (AECs) are important for initiating the host response against S. pneumoniae, and we evaluated the role of Toll-like receptor (TLR) 2, TLR4, and p38 mitogen-activated protein kinase (MAPK) signaling in the inflammatory response after pneumococcal infection. We established a novel model of acute S. pneumoniae infection using vital human lung specimens. In situ hybridization analysis showed that S. pneumoniae DNA was detected in 80 to 90% of AMs and 15 to 30% of AECs after in vitro infection accompanied by increased expression of inflammatory cytokines. Enhanced phosphorylation of p38 MAPK and increased TLR2 and 4 mRNA expression were observed in infected lung tissue. Thirty to fifty percent of AMs and 10 to 20% of AECs showed evidence of apoptosis 24 hours after pneumococcal infection. After macrophage deactivation with Clodronate/liposomes, infected lung tissue exhibited a significantly decreased release of inflammatory mediators. Inhibition of p38 MAPK signaling markedly reduced inflammatory cytokine release from human lungs, whereas TLR2 blockade revealed only minor effects. AMs are central resident immune cells during S. pneumoniae infection and are the main source of early proinflammatory cytokine release. p38 MAPK holds a major role in pathogen-induced pulmonary cytokine release and is a potential molecular target to modulate overwhelming lung inflammation.", "gemini": "Streptococcus pneumoniae là tác nhân gây bệnh chính của viêm phổi mắc phải trong cộng đồng và là nguyên nhân chính gây tử vong do nhiễm trùng. Tuy nhiên, người ta biết rất ít về sự tương tác giữa vật chủ và mầm bệnh ở mô phổi người. Chúng tôi đã kiểm tra giả thuyết rằng các đại thực bào phế nang (AM) và các tế bào biểu mô phế nang (AEC) của người rất quan trọng để khởi phát phản ứng của vật chủ chống lại S. pneumoniae và chúng tôi đã đánh giá vai trò của thụ thể giống Toll (TLR) 2, TLR4 và mitogen- hoạt hóa protein kinase (MAPK) p38 báo hiệu trong phản ứng viêm sau khi nhiễm phế cầu khuẩn. Chúng tôi đã thiết lập một mô hình mới về nhiễm trùng S. pneumoniae cấp tính bằng cách sử dụng các mẫu phổi người còn sống. Phân tích lai in situ cho thấy DNA S. pneumoniae được phát hiện ở 80 đến 90% AM và 15 đến 30% AEC sau khi nhiễm in vitro kèm theo biểu hiện tăng các cytokine gây viêm. Sự phosphoryl hóa tăng cường của p38 MAPK và biểu hiện mRNA TLR2 và 4 tăng lên được quan sát thấy ở mô phổi bị nhiễm bệnh. Ba mươi đến năm mươi phần trăm AM và 10 đến 20% AEC cho thấy bằng chứng của quá trình apoptosis 24 giờ sau khi nhiễm phế cầu khuẩn. Sau khi vô hiệu hóa đại thực bào bằng Clodronate/liposome, mô phổi bị nhiễm bệnh biểu hiện giải phóng các chất trung gian gây viêm giảm đáng kể. Việc ức chế tín hiệu p38 MAPK làm giảm đáng kể giải phóng cytokine gây viêm từ phổi người, trong khi sự phong bế TLR2 chỉ cho thấy những tác động nhỏ. AM là các tế bào miễn dịch trú trung tâm trong quá trình nhiễm S. pneumoniae và là nguồn chính giải phóng cytokine tiền viêm sớm. p38 MAPK đóng vai trò chính trong giải phóng cytokine phổi do tác nhân gây bệnh và là mục tiêu phân tử tiềm năng để điều chỉnh tình trạng viêm phổi quá mức."} +{"idx": 1960017, "en": "The nose is an attractive source of airway epithelial cells, particularly in populations in which bronchoscopy may not be possible. However, substituting nasal cells for bronchial epithelial cells in the study of airway inflammation depends upon comparability of responses, and evidence for this is lacking. Our objective was to determine whether nasal epithelial cell inflammatory mediator release and receptor expression reflect those of bronchial epithelial cells. Paired cultures of undifferentiated nasal and bronchial epithelial cells were obtained from brushings from 35 subjects, including 5 children. Cells were subject to morphologic and immunocytochemical assessment. Mediator release from resting and cytokine-stimulated cell monolayers was determined, as was cell surface receptor expression. Nasal and bronchial cells had identical epithelial morphology and uniform expression of cytokeratin 19. There were no differences in constitutive expression of CD44, intercellular adhesion molecule-1, alphavbeta3, and alphavbeta5. Despite significantly higher constitutive release of IL-8, IL-6, RANTES (regulated on activation, normal T cell expressed and secreted), and matrix metalloproteinase (MMP)-9 from nasal compared with bronchial cells, the increments in release of all studied mediators in response to stimulation with IL-1beta and TNF-alpha were similar, and there were significant positive correlations between nasal and bronchial cell secretion of IL-6, RANTES, vascular endothelial growth factor, monocyte chemoattractant protein-1, MMP-9, and tissue inhibitor of metalloproteinase-1. Despite differences in absolute mediator levels, the responses of nasal and bronchial epithelial cells to cytokine stimulation were similar, expression of relevant surface receptors was comparable, and there were significant correlations between nasal and bronchial cell mediator release. Therefore, nasal epithelial cultures constitute an accessible surrogate for studying lower airway inflammation.", "gemini": "Mũi là một nguồn cung cấp các tế bào biểu mô đường thở hấp dẫn, đặc biệt là ở những quần thể mà nội soi phế quản có thể không thực hiện được. Tuy nhiên, việc thay thế các tế bào mũi bằng các tế bào biểu mô phế quản trong nghiên cứu về tình trạng viêm đường thở phụ thuộc vào khả năng so sánh các phản ứng và bằng chứng cho điều này còn thiếu. Mục tiêu của chúng tôi là xác định xem giải phóng chất trung gian gây viêm của tế bào biểu mô mũi và biểu hiện thụ thể có phản ánh các tế bào biểu mô phế quản hay không. Các nuôi cấy ghép đôi các tế bào biểu mô mũi và phế quản chưa biệt hóa được lấy từ các lần chải của 35 đối tượng, bao gồm 5 trẻ em. Các tế bào được đánh giá về mặt hình thái và miễn dịch tế bào hóa học. Sự giải phóng chất trung gian từ các lớp tế bào đơn lớp đang nghỉ ngơi và được kích thích bằng cytokine đã được xác định, cũng như biểu hiện thụ thể bề mặt tế bào. Các tế bào mũi và phế quản có hình thái biểu mô giống hệt nhau và biểu hiện đồng nhất của cytokeratin 19. Không có sự khác biệt về biểu hiện cấu thành của CD44, phân tử liên kết tế bào gian bào-1, alphavbeta3 và alphavbeta5. Mặc dù giải phóng cấu thành cao hơn đáng kể của IL-8, IL-6, RANTES (được điều chỉnh khi hoạt hóa, tế bào T bình thường biểu hiện và tiết ra) và metalloproteinase ma trận (MMP)-9 từ các tế bào mũi so với các tế bào phế quản, nhưng sự gia tăng giải phóng tất cả các chất trung gian đã nghiên cứu để đáp ứng với sự kích thích bằng IL-1beta và TNF-alpha là tương tự nhau và có mối tương quan dương đáng kể giữa tiết IL-6, RANTES, yếu tố tăng trưởng nội mô mạch máu, protein hấp dẫn bạch cầu đơn nhân-1, MMP-9 và chất ức chế mô của metalloproteinase-1 của tế bào mũi và phế quản. Mặc dù có sự khác biệt về mức chất trung gian tuyệt đối, nhưng phản ứng của các tế bào biểu mô mũi và phế quản đối với kích thích cytokine là tương tự nhau, biểu hiện của các thụ thể bề mặt có liên quan là tương đương nhau và có mối tương quan đáng kể giữa giải phóng chất trung gian của tế bào mũi và phế quản. Do đó, các nuôi cấy biểu mô mũi tạo thành một vật thay thế dễ tiếp cận để nghiên cứu tình trạng viêm đường thở dưới."} +{"idx": 1960018, "en": "Elucidating the underlying transcriptional control of pluripotent cells is necessary for the development of new methods of inducing and maintaining pluripotent cells in vitro. Three transcription factors, Nanog, Oct4, and Sox2, have been reported to form a feedforward circuit promoting pluripotent cell self-renewal in embryonic stem cells (ESC). Previously, we found that a transcriptional repressor activity of Tcf3, a DNA-binding effector of Wnt signaling, reduced Nanog promoter activity and Nanog levels in mouse embryonic stem cells (mESC). The objective of this study was to determine the scope of Tcf3 effects on gene expression and self-renewal beyond the regulation of Nanog levels. We show that Tcf3 acts broadly on a genome-wide scale to reduce the levels of several promoters of self-renewal (Nanog, Tcl1, Tbx3, Esrrb) while not affecting other ESC genes (Oct4, Sox2, Fgf4). Comparing effects of Tcf3 ablation with Oct4 or Nanog knockdown revealed that Tcf3 counteracted effects of both Nanog and Oct4. Interestingly, the effects of Tcf3 were more strongly correlated with Oct4 than with Nanog, despite the normal levels of Oct4 in TCF3-/- mESC. The deranged gene expression allowed TCF3-/- mESC self-renewal even in the absence of leukemia inhibitory factor and delayed differentiation in embryoid bodies. These findings identify Tcf3 as a cell-intrinsic inhibitor of pluripotent cell self-renewal that functions by limiting steady-state levels of self-renewal factors. Disclosure of potential conflicts of interest is found at the end of this article.", "gemini": "Việc làm sáng tỏ sự kiểm soát phiên mã cơ bản của các tế bào đa năng là cần thiết để phát triển các phương pháp mới để gây ra và duy trì các tế bào đa năng trong ống nghiệm. Ba yếu tố phiên mã, Nanog, Oct4 và Sox2, đã được báo cáo là tạo thành một mạch phản hồi thúc đẩy sự tự tái tạo của tế bào đa năng trong các tế bào gốc phôi (ESC). Trước đây, chúng tôi phát hiện ra rằng hoạt động ức chế phiên mã của Tcf3, một tác nhân liên kết DNA của tín hiệu Wnt, làm giảm hoạt động của trình thúc đẩy Nanog và mức độ Nanog trong các tế bào gốc phôi chuột (mESC). Mục tiêu của nghiên cứu này là xác định phạm vi tác động của Tcf3 lên biểu hiện gen và sự tự tái tạo vượt ra ngoài sự điều chỉnh mức độ Nanog. Chúng tôi chỉ ra rằng Tcf3 hoạt động rộng rãi trên quy mô toàn bộ hệ gen để giảm mức độ của một số trình thúc đẩy sự tự tái tạo (Nanog, Tcl1, Tbx3, Esrrb) trong khi không ảnh hưởng đến các gen ESC khác (Oct4, Sox2, Fgf4). So sánh tác động của việc loại bỏ Tcf3 với việc loại bỏ Oct4 hoặc Nanog cho thấy Tcf3 chống lại tác động của cả Nanog và Oct4. Điều thú vị là các tác động của Tcf3 có mối tương quan mạnh hơn với Oct4 so với Nanog, mặc dù mức độ Oct4 bình thường ở TCF3-/- mESC. Biểu hiện gen bị rối loạn cho phép TCF3-/- mESC tự tái tạo ngay cả khi không có yếu tố ức chế bệnh bạch cầu và trì hoãn sự biệt hóa trong các thể phôi. Những phát hiện này xác định Tcf3 là chất ức chế nội tại tế bào đối với sự tự tái tạo của tế bào đa năng hoạt động bằng cách giới hạn mức độ ổn định của các yếu tố tự tái tạo. Việc tiết lộ các xung đột lợi ích tiềm ẩn được tìm thấy ở cuối bài viết này."} +{"idx": 1960019, "en": "The isolation of human embryonic and somatic stem cells of different types has made it possible to design novel gene and cell replacement therapies. Vectors derived from retro/lentiviruses are used to stably introduce genes into stem cells and their progeny. However, the permissivity to retroviral infection varies among cell types. We previously showed that hematopoietic stem cells are poorly permissive to human immunodeficiency virus (HIV)-derived vectors and that pharmacological inhibition of the proteasome strongly enhances gene transfer. Here we report that the proteasome limits lentiviral gene transfer in all stem cell types tested, including embryonic, mesenchymal, and neural, of both human and mouse origin. Remarkably, this inhibitory activity was sharply reduced upon differentiation of the stem cells, suggesting that it represents a novel feature of the stem cell/immature progenitor phenotype. Proteasome-mediated inhibition was specific for lentiviral vectors and occurred at a postentry infection step. It was not mediated by activation of nuclear factor-kappaB, a major signaling pathway modulated by the proteasome, and did not correlate with high proteasome activity. Interaction of the virion core with cyclophilin A was required to maximize the effect of proteasome inhibitor on the infection pathway. These findings are relevant to uncover new mediators of HIV gene transfer and help in designing more effective protocols for the genetic modification of stem cells. Disclosure of potential conflicts of interest is found at the end of this article.", "gemini": "Việc phân lập các tế bào gốc phôi và tế bào gốc sinh dưỡng của con người thuộc các loại khác nhau đã giúp có thể thiết kế các liệu pháp thay thế gen và tế bào mới. Các vectơ có nguồn gốc từ retro/lentivirus được sử dụng để đưa gen vào các tế bào gốc và các tế bào con của chúng một cách ổn định. Tuy nhiên, khả năng cho phép nhiễm retrovirus khác nhau giữa các loại tế bào. Trước đây, chúng tôi đã chỉ ra rằng các tế bào gốc tạo máu kém cho phép các vectơ có nguồn gốc từ vi-rút gây suy giảm miễn dịch ở người (HIV) và việc ức chế proteasome bằng dược lý làm tăng đáng kể quá trình chuyển gen. Ở đây chúng tôi báo cáo rằng proteasome hạn chế quá trình chuyển gen lentivirus ở tất cả các loại tế bào gốc đã thử nghiệm, bao gồm phôi, trung mô và thần kinh, có nguồn gốc từ cả người và chuột. Đáng chú ý, hoạt động ức chế này giảm mạnh khi các tế bào gốc biệt hóa, cho thấy rằng nó đại diện cho một đặc điểm mới của kiểu hình tiền thân chưa trưởng thành/tế bào gốc. Sự ức chế do proteasome trung gian là đặc hiệu đối với các vectơ lentivirus và xảy ra ở bước nhiễm trùng sau khi xâm nhập. Nó không được trung gian bởi sự hoạt hóa của yếu tố hạt nhân kappaB, một con đường truyền tín hiệu chính được điều chỉnh bởi proteasome, và không tương quan với hoạt động proteasome cao. Sự tương tác của lõi virion với cyclophilin A là cần thiết để tối đa hóa tác dụng của chất ức chế proteasome trên con đường lây nhiễm. Những phát hiện này có liên quan đến việc khám phá ra các chất trung gian mới của quá trình chuyển gen HIV và giúp thiết kế các giao thức hiệu quả hơn để biến đổi gen của các tế bào gốc. Việc tiết lộ các xung đột lợi ích tiềm ẩn được tìm thấy ở cuối bài viết này."} +{"idx": 1960020, "en": "During bacterial infection, the bone marrow hematopoietic activity shifts toward granulocyte production, which is critical for host defenses. Along with this enhancement of granulopoiesis, the bone marrow also increases its release of hematopoietic precursors. At the present time, little is known about the commitment of hematopoietic precursor cells, including hematopoietic stem cells and progenitors, in this response. To investigate the hematopoietic precursor cell response to bacterial infection, bacteremia was established in Balb/c mice by i.v. injection of Escherichia coli. Bacteremia caused a 10-fold increase in the number of lineage (lin)-c-kit+Sca-1+ cells in the bone marrow. This dramatic expansion of the lin-c-kit+Sca-1+ cell pool resulted from both increased mitosis of these cells and inversion from lin-c-kit+Sca-1- cell phenotype. Lipopolysaccharide, tumor necrosis factor-alpha, and interleukin-6 were potent factors capable of mediating phenotypic inversion of lin-c-kit+Sca-1- cells. Cells in the expanded lin-c-kit+Sca-1+ cell pool contained more colony-forming unit-granulocyte/macrophage. Mobilization of lin-c-kit+Sca-1+ cells into the circulation was significantly enhanced following bacteremia. These results demonstrate that the lin-c-kit+Sca-1+ cell population in the bone marrow constitutes a key component of the host defense response to bacteremia. Functional modifications of these primitive hematopoietic precursors are critical for enhancing granulocyte production following bacterial infection.", "gemini": "Trong quá trình nhiễm khuẩn, hoạt động tạo máu của tủy xương chuyển sang sản xuất bạch cầu hạt, điều này rất quan trọng đối với khả năng phòng vệ của vật chủ. Cùng với sự tăng cường sinh bạch cầu hạt này, tủy xương cũng tăng giải phóng các tiền thân tạo máu. Hiện tại, người ta biết rất ít về sự cam kết của các tế bào tiền thân tạo máu, bao gồm cả tế bào gốc tạo máu và các tế bào tiền thân, trong phản ứng này. Để điều tra phản ứng của tế bào tiền thân tạo máu đối với nhiễm khuẩn, tình trạng nhiễm khuẩn huyết đã được thiết lập ở chuột Balb/c bằng cách tiêm tĩnh mạch Escherichia coli. Nhiễm khuẩn huyết gây ra sự gia tăng gấp 10 lần số lượng tế bào dòng (lin)-c-kit+Sca-1+ trong tủy xương. Sự mở rộng đáng kể này của nhóm tế bào lin-c-kit+Sca-1+ là kết quả của cả quá trình nguyên phân tăng lên của các tế bào này và sự đảo ngược từ kiểu hình tế bào lin-c-kit+Sca-1-. Lipopolysaccharide, yếu tố hoại tử khối u-alpha và interleukin-6 là những yếu tố mạnh có khả năng trung gian sự đảo ngược kiểu hình của các tế bào lin-c-kit+Sca-1-. Các tế bào trong nhóm tế bào lin-c-kit+Sca-1+ mở rộng chứa nhiều hơn đơn vị tạo khuẩn lạc-bạch cầu hạt/đại thực bào. Sự huy động các tế bào lin-c-kit+Sca-1+ vào tuần hoàn được tăng cường đáng kể sau khi bị nhiễm khuẩn huyết. Những kết quả này chứng minh rằng quần thể tế bào lin-c-kit+Sca-1+ trong tủy xương là một thành phần chính của phản ứng phòng vệ của vật chủ đối với tình trạng nhiễm khuẩn huyết. Các sửa đổi chức năng của các tiền thân tạo máu nguyên thủy này rất quan trọng để tăng cường sản xuất bạch cầu hạt sau khi nhiễm khuẩn."} +{"idx": 1960021, "en": "Derived from Buddhism, mindfulness is a unique approach for understanding human suffering and happiness that has attracted rapidly growing interest among health care professionals. In this article I describe current thinking about the concept of mindfulness and elaborate on why and how mindfulness-based interventions have potential within the context of geriatric medicine and gerontology. Upon reviewing definitions and models of the concept, I give attention to the unique role that the body plays in cultivating mindfulness and the advantages that this focus has for older adults because they have aging biological systems and may experience chronic disease, pain, and disability. In the final section I discuss why mindfulness may be particularly useful in promoting physical activity among older adults and how physical activity may be used as a vehicle to promote mindfulness.", "gemini": "Xuất phát từ Phật giáo, chánh niệm là một phương pháp tiếp cận độc đáo để hiểu về nỗi khổ và hạnh phúc của con người, đã thu hút sự quan tâm ngày càng lớn trong số các chuyên gia chăm sóc sức khỏe. Trong bài viết này, tôi mô tả cách suy nghĩ hiện tại về khái niệm chánh niệm và trình bày chi tiết về lý do tại sao và làm thế nào các can thiệp dựa trên chánh niệm có tiềm năng trong bối cảnh y học lão khoa và lão khoa học. Khi xem xét các định nghĩa và mô hình của khái niệm, tôi chú ý đến vai trò độc đáo mà cơ thể đóng trong việc nuôi dưỡng chánh niệm và những lợi thế mà trọng tâm này mang lại cho người lớn tuổi vì họ có hệ thống sinh học lão hóa và có thể mắc các bệnh mãn tính, đau đớn và khuyết tật. Trong phần cuối, tôi thảo luận về lý do tại sao chánh niệm có thể đặc biệt hữu ích trong việc thúc đẩy hoạt động thể chất ở người lớn tuổi và làm thế nào hoạt động thể chất có thể được sử dụng như một phương tiện để thúc đẩy chánh niệm."} +{"idx": 1960022, "en": "This article uses a retrospective approach to critique the research base underlying the nursing home culture-change movement-an effort to radically transform the nation's nursing homes by delivering resident-directed care and empowering staff. The article traces the development of the movement from its inception 10 years ago to 2005, when the Centers for Medicare and Medicaid Services implemented its own initiative to support the movement, thus giving it new momentum, to the present day. This historical overview provides context for a proposed research agenda aimed at strengthening the movement's empirical base, thereby facilitating culture-change interventions as well as helping the movement navigate the next step in its evolution.", "gemini": "Bài viết này sử dụng phương pháp tiếp cận hồi cứu để phê bình cơ sở nghiên cứu đằng sau phong trào thay đổi văn hóa viện dưỡng lão - một nỗ lực nhằm thay đổi hoàn toàn các viện dưỡng lão của quốc gia bằng cách cung cấp dịch vụ chăm sóc theo chỉ đạo của cư dân và trao quyền cho nhân viên. Bài viết lần theo sự phát triển của phong trào từ khi bắt đầu cách đây 10 năm đến năm 2005, khi Trung tâm Dịch vụ Medicare và Medicaid triển khai sáng kiến riêng của mình để hỗ trợ phong trào, do đó tạo động lực mới cho phong trào cho đến ngày nay. Tổng quan lịch sử này cung cấp bối cảnh cho một chương trình nghị sự nghiên cứu được đề xuất nhằm mục đích củng cố cơ sở thực nghiệm của phong trào, do đó tạo điều kiện thuận lợi cho các can thiệp thay đổi văn hóa cũng như giúp phong trào điều hướng bước tiếp theo trong quá trình phát triển của mình."} +{"idx": 1960023, "en": "PURPOSE Remembering medical treatment information may be particularly taxing for older cancer patients, but to our knowledge this ability has never been assessed in this specific age group only. Our purpose in this study was to investigate older cancer patients' recall of information after patient education preceding chemotherapy. DESIGN AND METHODS We constructed a recall questionnaire consisting of multiple-choice questions, completion items, and open-ended questions related to information about treatment and recommendations on how to handle side effects. Immediately after a nursing consultation preceding chemotherapy treatment, 69 older patients (M = 71.8 years, SD = 4.1) completed the questionnaire. We checked recall against the actual communication in video recordings of the consultations. RESULTS On average, 82.2 items were discussed during the consultations. The mean percentage of information recalled correctly was 23.2% for open-ended questions, 68.0% for completion items, and 80.2% for multiple-choice questions. IMPLICATIONS Older cancer patients are confronted with a lot of information. Recall of information strongly depended on question format; especially active reproduction appeared to be poor. To improve treatment outcomes, it is important that cancer patients are able to actively retrieve knowledge about how to prevent and recognize adverse side effects and that this is checked by the health professional. We make suggestions on how to make information more memorable for older cancer patients.", "gemini": "MỤC ĐÍCH Ghi nhớ thông tin điều trị y tế có thể đặc biệt khó khăn đối với bệnh nhân ung thư lớn tuổi, nhưng theo hiểu biết của chúng tôi, khả năng này chưa bao giờ được đánh giá chỉ ở nhóm tuổi cụ thể này. Mục đích của chúng tôi trong nghiên cứu này là điều tra khả năng nhớ lại thông tin của bệnh nhân ung thư lớn tuổi sau khi giáo dục bệnh nhân trước khi hóa trị. THIẾT KẾ VÀ PHƯƠNG PHÁP Chúng tôi đã xây dựng một bảng câu hỏi nhớ lại bao gồm các câu hỏi trắc nghiệm, các mục hoàn thành và các câu hỏi mở liên quan đến thông tin về phương pháp điều trị và các khuyến nghị về cách xử lý các tác dụng phụ. Ngay sau khi tư vấn điều dưỡng trước khi điều trị hóa trị, 69 bệnh nhân lớn tuổi (M = 71,8 tuổi, SD = 4,1) đã hoàn thành bảng câu hỏi. Chúng tôi đã kiểm tra khả năng nhớ lại so với nội dung giao tiếp thực tế trong các bản ghi video của các buổi tư vấn. KẾT QUẢ Trung bình, 82,2 mục đã được thảo luận trong các buổi tư vấn. Tỷ lệ phần trăm trung bình của thông tin được nhớ lại chính xác là 23,2% đối với câu hỏi mở, 68,0% đối với câu hỏi hoàn thành và 80,2% đối với câu hỏi trắc nghiệm. Ý NGHĨA Bệnh nhân ung thư lớn tuổi phải đối mặt với rất nhiều thông tin. Khả năng nhớ lại thông tin phụ thuộc rất nhiều vào định dạng câu hỏi; đặc biệt là khả năng tái tạo tích cực dường như kém. Để cải thiện kết quả điều trị, điều quan trọng là bệnh nhân ung thư phải có khả năng chủ động tìm kiếm kiến thức về cách phòng ngừa và nhận biết các tác dụng phụ bất lợi và điều này được chuyên gia y tế kiểm tra. Chúng tôi đưa ra các đề xuất về cách làm cho thông tin đáng nhớ hơn đối với bệnh nhân ung thư lớn tuổi."} +{"idx": 1960024, "en": "PURPOSE Emphasis on consumer-centered care for frail and institutionalized older adults has increased the development and adaptation of surveys for this population. Conventional methods used to pretest survey items fail to investigate underlying sources of measurement error. However, the use of the cognitive interview (CI), a method for studying how respondents answer survey items, is not well established or documented in this population. This study demonstrates how CIs can be used to improve questionnaires intended for nursing home residents. DESIGN AND METHODS CIs were conducted with 29 nursing home residents in order to identify potential problems with prospective survey items. We used scripted probes to standardize the interviews and adapted the Question Appraisal System to enumerate and classify the problems discovered. RESULTS We fielded between one and five versions of each item in an iterative process that identified 61 item-specific problems. Additionally, residents' cognitive responses suggested that some screened their answers on the basis of perceived physical and environmental limitations, and some had difficulty answering items about preferences that fluctuate day to day. These findings led us to modify the items and response set to simplify the respondents' cognitive task. IMPLICATIONS This study illustrates how CI techniques can be used to understand residents' comprehension of and response to survey items.", "gemini": "MỤC ĐÍCH Việc nhấn mạnh vào chăm sóc lấy người tiêu dùng làm trung tâm đối với người cao tuổi yếu ớt và được đưa vào viện dưỡng lão đã làm tăng sự phát triển và điều chỉnh các cuộc khảo sát cho đối tượng này. Các phương pháp thông thường được sử dụng để thử nghiệm trước các mục khảo sát không điều tra được các nguồn gốc tiềm ẩn của lỗi đo lường. Tuy nhiên, việc sử dụng phỏng vấn nhận thức (CI), một phương pháp để nghiên cứu cách người trả lời trả lời các mục khảo sát, chưa được thiết lập hoặc ghi chép đầy đủ trong đối tượng này. Nghiên cứu này chứng minh cách CI có thể được sử dụng để cải thiện các bảng câu hỏi dành cho cư dân viện dưỡng lão. THIẾT KẾ VÀ PHƯƠNG PHÁP CI đã được thực hiện với 29 cư dân viện dưỡng lão để xác định các vấn đề tiềm ẩn với các mục khảo sát tiềm năng. Chúng tôi đã sử dụng các câu hỏi thăm dò có kịch bản để chuẩn hóa các cuộc phỏng vấn và điều chỉnh Hệ thống đánh giá câu hỏi để liệt kê và phân loại các vấn đề được phát hiện. KẾT QUẢ Chúng tôi đã đưa ra từ một đến năm phiên bản của mỗi mục trong một quá trình lặp đi lặp lại đã xác định được 61 vấn đề cụ thể của từng mục. Ngoài ra, các phản ứng nhận thức của cư dân cho thấy một số người đã sàng lọc câu trả lời của họ trên cơ sở nhận thức về những hạn chế về thể chất và môi trường, và một số người gặp khó khăn khi trả lời các câu hỏi về sở thích thay đổi từng ngày. Những phát hiện này khiến chúng tôi phải sửa đổi các mục và bộ câu trả lời để đơn giản hóa nhiệm vụ nhận thức của người trả lời. Ý NGHĨA Nghiên cứu này minh họa cách các kỹ thuật CI có thể được sử dụng để hiểu được khả năng hiểu và phản hồi của cư dân đối với các mục khảo sát."} +{"idx": 1960025, "en": "PURPOSE As consensus emerges concerning the need to extend publicly funded home- and community-based services that support the independence of seniors, studies have reported the efficacy and cost effectiveness of assistive technology (AT). This article presents the latest available national AT expenditure and participation trends (1999-2002) for Medicaid 1915(c) waivers, the largest Medicaid home- and community-based service program. DESIGN AND METHODS We collected annually reported Centers for Medicare and Medicaid Form 372 data from state officials for each waiver providing AT for the period from 1999 to 2002. Descriptive statistics examined trends in national participation and expenditures, interstate variations in participation and expenditures, and differences in provision between elderly persons and persons with developmental disabilities. RESULTS Although we report a rise in the number of waivers providing AT, there has been much slower participant growth compared with the broader waiver program, and there is wide interstate variation in waiver AT provision. Not only do most waivers with AT serve persons with developmental disabilities, AT spending for that target group is almost twice that for aged or disabled waiver participants. IMPLICATIONS This study highlights three policy concerns: first, the large interstate variations in AT provision in Medicaid waivers may signal access problems in some states; second, policy choices in some states may favor Medicaid spending on AT for the developmental disability population over that for the elderly population; and third, data limitations prevent a comparable state-by-state analysis of Medicare AT provision.", "gemini": "MỤC ĐÍCH Khi sự đồng thuận nổi lên liên quan đến nhu cầu mở rộng các dịch vụ hỗ trợ sự độc lập của người cao tuổi do nhà nước tài trợ và dựa trên cộng đồng, các nghiên cứu đã báo cáo về hiệu quả và hiệu quả về chi phí của công nghệ hỗ trợ (AT). Bài viết này trình bày các xu hướng chi tiêu và tham gia AT quốc gia mới nhất (1999-2002) đối với các miễn trừ Medicaid 1915(c), chương trình dịch vụ dựa trên cộng đồng và tại nhà của Medicaid lớn nhất. THIẾT KẾ VÀ PHƯƠNG PHÁP Chúng tôi đã thu thập dữ liệu Biểu mẫu 372 của Trung tâm Medicare và Medicaid được các quan chức tiểu bang báo cáo hàng năm đối với mỗi đơn miễn trừ cung cấp AT trong giai đoạn từ 1999 đến 2002. Thống kê mô tả đã xem xét các xu hướng về tham gia và chi tiêu quốc gia, sự khác biệt giữa các tiểu bang về tham gia và chi tiêu, và sự khác biệt trong cung cấp giữa người cao tuổi và người khuyết tật về phát triển. KẾT QUẢ Mặc dù chúng tôi báo cáo rằng số lượng đơn miễn trừ cung cấp AT đã tăng lên, nhưng tốc độ tăng trưởng của người tham gia chậm hơn nhiều so với chương trình miễn trừ rộng hơn và có sự khác biệt lớn giữa các tiểu bang trong việc cung cấp AT miễn trừ. Hầu hết các đơn miễn trừ có AT không chỉ phục vụ những người khuyết tật về phát triển mà chi tiêu cho AT cho nhóm đối tượng đó còn gần gấp đôi so với những người tham gia miễn trừ là người già hoặc khuyết tật. Ý NGHĨA Nghiên cứu này nêu bật ba mối quan tâm về chính sách: thứ nhất, sự khác biệt lớn giữa các tiểu bang về cung cấp AT trong các đơn miễn trừ của Medicaid có thể báo hiệu các vấn đề về tiếp cận ở một số tiểu bang; thứ hai, các lựa chọn chính sách ở một số tiểu bang có thể ưu tiên chi tiêu Medicaid cho AT cho đối tượng khuyết tật về phát triển hơn là đối tượng người cao tuổi; và thứ ba, những hạn chế về dữ liệu ngăn cản việc phân tích so sánh giữa các tiểu bang về việc cung cấp AT của Medicare."} +{"idx": 1960026, "en": "PURPOSE The purposes of this study were (a) to identify cognitive abilities and other factors related to successful completion of training for computer-based tasks that simulated real jobs and (b) to create a brief assessment battery useful in assessing older adults for these kinds of jobs. DESIGN AND METHODS Participants from three age groups (young, middle-aged, and older) completed a battery of cognitive measures. They then trained on one of three computer-based tasks that simulated actual jobs and were asked to perform the tasks for 3 days. We recorded whether they completed training and whether and how well they did the tasks. In a series of logistic regressions, we evaluated the ability of a subset of cognitive measures drawn from a larger battery to predict participants' ability to successfully complete training and go on to task performance. RESULTS Results confirmed theory-based expectations that measures of domain knowledge, crystallized intelligence, memory, and psychomotor speed would predict success in computer-based activities. A brief battery was able to predict older adults' successful completion of training for one task but was less useful for another. IMPLICATIONS A brief battery of cognitive measures may be useful in evaluating individuals for job selection. Different measures are related to job-related criteria depending on task and group evaluated, although it was not possible to identify a reduced battery for one task. The specific cognitive abilities related to participants' success have implications for task and interface design for the elderly population.", "gemini": "MỤC ĐÍCH Mục đích của nghiên cứu này là (a) xác định các khả năng nhận thức và các yếu tố khác liên quan đến việc hoàn thành thành công khóa đào tạo các nhiệm vụ trên máy tính mô phỏng công việc thực tế và (b) tạo ra một bộ đánh giá ngắn hữu ích để đánh giá những người lớn tuổi cho các loại công việc này. THIẾT KẾ VÀ PHƯƠNG PHÁP Những người tham gia từ ba nhóm tuổi (trẻ, trung niên và già) đã hoàn thành một loạt các biện pháp nhận thức. Sau đó, họ được đào tạo về một trong ba nhiệm vụ trên máy tính mô phỏng các công việc thực tế và được yêu cầu thực hiện các nhiệm vụ trong 3 ngày. Chúng tôi ghi lại liệu họ có hoàn thành khóa đào tạo hay không và liệu họ có thực hiện các nhiệm vụ tốt như thế nào. Trong một loạt các hồi quy logistic, chúng tôi đã đánh giá khả năng của một tập hợp con các biện pháp nhận thức được rút ra từ một bộ lớn hơn để dự đoán khả năng hoàn thành khóa đào tạo thành công của người tham gia và tiếp tục thực hiện nhiệm vụ. KẾT QUẢ Kết quả xác nhận các kỳ vọng dựa trên lý thuyết rằng các biện pháp về kiến thức về lĩnh vực, trí thông minh đã được kết tinh, trí nhớ và tốc độ vận động sẽ dự đoán thành công trong các hoạt động trên máy tính. Một bộ câu hỏi ngắn đã có thể dự đoán được việc những người lớn tuổi hoàn thành khóa đào tạo thành công cho một nhiệm vụ nhưng lại ít hữu ích hơn cho nhiệm vụ khác. Ý NGHĨA Một bộ câu hỏi ngắn về các biện pháp nhận thức có thể hữu ích trong việc đánh giá các cá nhân để lựa chọn công việc. Các biện pháp khác nhau có liên quan đến các tiêu chí liên quan đến công việc tùy thuộc vào nhiệm vụ và nhóm được đánh giá, mặc dù không thể xác định được một bộ câu hỏi giảm cho một nhiệm vụ. Các khả năng nhận thức cụ thể liên quan đến thành công của người tham gia có ý nghĩa đối với thiết kế nhiệm vụ và giao diện cho đối tượng người cao tuổi."} +{"idx": 1960027, "en": "PURPOSE Although migration decision making is central to understanding later-life migration, the critical step between migration intentions and mobility outcomes has received only limited empirical attention. We address two questions: How often are intended moves actuated? What factors condition the likelihood that mobility intentions will be actuated? DESIGN AND METHODS We employ data from the 1994-2002 Health and Retirement Study, which is a nationally representative panel targeting households containing persons aged 53 to 63 years at baseline. Event-history techniques are used to examine the link between reported mobility intentions at baseline and mobility outcomes across the study period, net of relevant controls. We conduct separate household-level analyses for couple and noncouple households and recognize three types of moves: local, family oriented, and nonlocal. RESULTS Findings confirm the utility of mobility expectations as a predictor of future mobility. More importantly, results highlight the complex nature of later-life mobility. The actuation of mobility intentions appears to operate differently in couple than in noncouple households. Moreover, our findings suggest that the role of several key variables depends on the type of move under consideration. IMPLICATIONS The ability to identify potential \"retirement migrants\" may be of practical importance for state and local government officials as well as developers interested in recruiting or retaining young-old residents. Our study offers insight on the interpretation of stated mobility intentions. Moreover, consistent with early theoretical work in the field, our analysis suggests that empirical studies must account for heterogeneity among older movers in order to avoid misleading results.", "gemini": "MỤC ĐÍCH Mặc dù việc ra quyết định di cư đóng vai trò trung tâm trong việc hiểu về tình trạng di cư ở giai đoạn sau của cuộc đời, nhưng bước quan trọng giữa ý định di cư và kết quả di cư chỉ nhận được sự quan tâm thực nghiệm hạn chế. Chúng tôi giải quyết hai câu hỏi: Ý định chuyển đi thường xuyên được thực hiện như thế nào? Những yếu tố nào quyết định khả năng thực hiện ý định di chuyển? THIẾT KẾ VÀ PHƯƠNG PHÁP Chúng tôi sử dụng dữ liệu từ Nghiên cứu về Sức khỏe và Nghỉ hưu 1994-2002, đây là một nhóm đại diện toàn quốc nhắm vào các hộ gia đình có người từ 53 đến 63 tuổi khi bắt đầu. Các kỹ thuật về lịch sử sự kiện được sử dụng để kiểm tra mối liên hệ giữa các ý định di chuyển được báo cáo khi bắt đầu và kết quả di chuyển trong suốt thời gian nghiên cứu, không bao gồm các biện pháp kiểm soát có liên quan. Chúng tôi tiến hành các phân tích riêng ở cấp hộ gia đình đối với các hộ gia đình có cặp đôi và không có cặp đôi và nhận ra ba loại di chuyển: địa phương, theo định hướng gia đình và không phải địa phương. KẾT QUẢ Các phát hiện xác nhận tính hữu ích của kỳ vọng di chuyển như một yếu tố dự đoán khả năng di chuyển trong tương lai. Quan trọng hơn, kết quả làm nổi bật bản chất phức tạp của khả năng di chuyển ở giai đoạn sau của cuộc đời. Việc thực hiện ý định di chuyển dường như diễn ra khác nhau ở các hộ gia đình có cặp đôi so với các hộ gia đình không có cặp đôi. Hơn nữa, những phát hiện của chúng tôi cho thấy rằng vai trò của một số biến chính phụ thuộc vào loại di chuyển đang xem xét. Ý NGHĨA Việc có khả năng xác định được những \"người di cư nghỉ hưu\" tiềm năng có thể có tầm quan trọng thực tế đối với các quan chức chính quyền tiểu bang và địa phương cũng như những nhà phát triển có hứng thú trong việc tuyển dụng hoặc giữ chân những cư dân trẻ tuổi. Nghiên cứu của chúng tôi đưa ra cái nhìn sâu sắc về cách giải thích các ý định di chuyển đã nêu. Hơn nữa, phù hợp với công trình lý thuyết ban đầu trong lĩnh vực này, phân tích của chúng tôi cho thấy rằng các nghiên cứu thực nghiệm phải tính đến tính không đồng nhất giữa những người di chuyển lớn tuổi để tránh đưa ra kết quả gây hiểu lầm."} +{"idx": 1960028, "en": "PURPOSE Researchers know little about the physical performance ability of residential care/assisted living (RC/AL) residents and its relationship to adverse outcomes such as fracture, nursing home placement, functional decline, and death. The purposes of this article are to (a) describe the functional characteristics of RC/AL residents, (b) examine the relationships between resident- and facility-level characteristics and physical performance, and (c) determine the predictive value of physical performance for adverse outcomes. DESIGN AND METHODS Data came from 1,791 residents in 189 RC/AL facilities participating in the Collaborative Studies of Long-Term Care. At baseline, residents were tested on four performance measures (grip strength, chair rise, balance, and walking speed), and other resident- and facility-level information was collected. Adverse outcomes were measured over 1 year. RESULTS Average grip strength was 14 +/- 7 kg, 61% of residents walked <0.6 m/s (M = 0.41 m/s), 26% could perform five chair rises, and only 19% could perform a tandem stand for a least 1 s. Multivariable analyses showed that more cognitive and functional impairment, depressive symptoms and comorbid conditions, and for-profit ownership were associated with poorer physical performance. Controlling for individual characteristics, we found that better performance on the four physical performance measures was associated with a reduced risk of nursing home placement, fracture, and decline in function over 1 year. IMPLICATIONS Simple performance measures identify modifiable functional deficits and suggest targeted interventions to prolong independent mobility and aging in place in RC/AL facilities.", "gemini": "MỤC ĐÍCH Các nhà nghiên cứu biết rất ít về khả năng hoạt động thể chất của những người cư trú tại cơ sở chăm sóc tại nhà/sinh hoạt hỗ trợ (RC/AL) và mối quan hệ của nó với các kết cục bất lợi như gãy xương, chuyển đến viện dưỡng lão, suy giảm chức năng và tử vong. Mục đích của bài viết này là (a) mô tả các đặc điểm chức năng của những người cư trú tại RC/AL, (b) kiểm tra mối quan hệ giữa các đặc điểm ở cấp độ cư dân và cơ sở với hoạt động thể chất và (c) xác định giá trị dự đoán của hoạt động thể chất đối với các kết cục bất lợi. THIẾT KẾ VÀ PHƯƠNG PHÁP Dữ liệu thu được từ 1.791 cư dân tại 189 cơ sở RC/AL tham gia Nghiên cứu Cộng tác về Chăm sóc Dài hạn. Khi bắt đầu, những người cư trú được kiểm tra bốn biện pháp đánh giá hiệu suất (sức nắm tay, đứng dậy khỏi ghế, thăng bằng và tốc độ đi bộ) và thu thập các thông tin khác về cư dân và cơ sở. Các kết cục bất lợi được đo trong vòng 1 năm. KẾT QUẢ Sức nắm tay trung bình là 14 +/- 7 kg, 61% cư dân đi bộ <0,6 m/giây (M = 0,41 m/giây), 26% có thể đứng dậy khỏi ghế năm lần và chỉ 19% có thể đứng song song trong ít nhất 1 giây. Các phân tích đa biến cho thấy suy giảm nhận thức và chức năng nhiều hơn, các triệu chứng trầm cảm và các tình trạng bệnh đi kèm, và sở hữu vì mục đích lợi nhuận có liên quan đến hoạt động thể chất kém hơn. Kiểm soát các đặc điểm cá nhân, chúng tôi thấy rằng hiệu suất tốt hơn ở bốn biện pháp đánh giá hoạt động thể chất có liên quan đến việc giảm nguy cơ chuyển đến viện dưỡng lão, gãy xương và suy giảm chức năng trong hơn 1 năm. Ý NGHĨA Các biện pháp đánh giá hiệu suất đơn giản xác định được các khiếm khuyết về chức năng có thể sửa đổi và gợi ý các biện pháp can thiệp có mục tiêu để kéo dài khả năng vận động độc lập và già hóa tại chỗ trong các cơ sở RC/AL."} +{"idx": 1960029, "en": "PURPOSE The purpose of this study was to estimate hospital and long-term-care costs resulting from falls in long-term-care facilities (LTCFs). DESIGN AND METHODS The study used a retrospective, pre/post with comparison group design. We used matching, based on propensity scores, to control for baseline differences between fallers and non-fallers. We estimated residents' propensity to fall from demographics, comorbidities, and reimbursement in the pre-period. The matched sample included 1,130 fallers and 1,130 non-fallers who were residents of a large, multifacility long-term-care chain. Cost estimates were based on information in the Minimum Data Set and were defined as hospital and LTCF reimbursements. We estimated fall-related costs as the difference between changes in costs for fallers and non-fallers from the pre- to post-period. RESULTS Fallers were substantially more likely to suffer fractures and hospitalizations in the post-period than were non-fallers. Fall-related LTCF and hospital costs were $6,259 (95% confidence interval = $2,034-$10,484) per resident per year. About 60% of this amount was attributable to higher hospitalization costs. Fallers were more likely to be discharged to hospitals or to die. IMPLICATIONS Falls in LTCFs are associated with costs of about $6,200 per resident per year. These results provide baseline estimates that one may use to estimate the cost-effectiveness of interventions to reduce fall rates.", "gemini": "MỤC ĐÍCH Mục đích của nghiên cứu này là ước tính chi phí bệnh viện và chăm sóc dài hạn do ngã tại các cơ sở chăm sóc dài hạn (LTCF). THIẾT KẾ VÀ PHƯƠNG PHÁP Nghiên cứu sử dụng thiết kế hồi cứu, trước/sau có nhóm so sánh. Chúng tôi sử dụng phương pháp ghép nối, dựa trên điểm khuynh hướng, để kiểm soát sự khác biệt ban đầu giữa những người bị ngã và không bị ngã. Chúng tôi ước tính khuynh hướng ngã của cư dân dựa trên thông tin nhân khẩu học, bệnh đi kèm và hoàn trả trong giai đoạn trước. Mẫu ghép nối bao gồm 1.130 người bị ngã và 1.130 người không bị ngã, là cư dân của một chuỗi chăm sóc dài hạn đa cơ sở lớn. Ước tính chi phí dựa trên thông tin trong Bộ Dữ liệu Tối thiểu và được định nghĩa là các khoản hoàn trả cho bệnh viện và LTCF. Chúng tôi ước tính chi phí liên quan đến ngã là sự khác biệt giữa sự thay đổi chi phí cho những người bị ngã và không bị ngã từ giai đoạn trước đến giai đoạn sau. KẾT QUẢ Những người bị ngã có khả năng bị gãy xương và nhập viện cao hơn đáng kể trong giai đoạn sau so với những người không bị ngã. Chi phí LTCF và bệnh viện liên quan đến ngã là 6.259 đô la (khoảng tin cậy 95% = 2.034-10.484 đô la) cho mỗi cư dân mỗi năm. Khoảng 60% số tiền này là do chi phí nhập viện cao hơn. Những người bị ngã có nhiều khả năng được xuất viện đến bệnh viện hoặc tử vong. Ý NGHĨA Việc ngã tại LTCF có liên quan đến chi phí khoảng 6.200 đô la cho mỗi cư dân mỗi năm. Những kết quả này cung cấp ước tính cơ sở mà người ta có thể sử dụng để ước tính hiệu quả về chi phí của các biện pháp can thiệp nhằm giảm tỷ lệ ngã."} +{"idx": 1960030, "en": "PURPOSE Older adults are less likely than any other age group to use the federal Food Stamp Program. The personal and social costs of elderly diet insufficiency include disease exacerbation, depression, and increased hospitalization. In order to improve targeting and outreach efforts, this study identifies the characteristics of eligible older Americans who are not receiving food stamps and assesses the validity of the Andersen behavioral model for predicting impoverished older adults food stamp use. DESIGN AND METHODS We conducted a secondary analysis of the 2003 American Community Survey, which is a nationally representative survey with a response rate of 96.7%. We restricted our study subsample to the 14,724 impoverished American citizens who were aged 65 years and older. We used bivariate and logistic regression analyses to compare the 2,796 food stamp recipients with the 11,928 nonrecipients. RESULTS One in five impoverished older American citizens had received foods stamps in the preceding year. Female respondents, renters, younger respondents, disabled individuals, and those who received Supplemental Security Income or welfare were more likely to receive food stamps. The pseudo-R-square value indicated that the Andersen Behavioral Model explained 28% of the model's variability. IMPLICATIONS Improved targeting is needed to enhance older adults' participation rates. Mobile and satellite food stamp offices in lower income neighborhoods and other innovative outreach programs that collaborate with community partners could also improve access. With the vast majority of impoverished older adults not receiving food stamps, strategies such as these are extremely important to rectify this situation among the most vulnerable group of older Americans.", "gemini": "MỤC ĐÍCH Người lớn tuổi ít có khả năng sử dụng Chương trình tem phiếu thực phẩm liên bang hơn bất kỳ nhóm tuổi nào khác. Các chi phí cá nhân và xã hội của việc ăn uống không đủ ở người cao tuổi bao gồm tình trạng bệnh trầm trọng hơn, trầm cảm và tăng số lần nhập viện. Để cải thiện các nỗ lực nhắm mục tiêu và tiếp cận, nghiên cứu này xác định các đặc điểm của những người Mỹ cao tuổi đủ điều kiện không nhận được tem phiếu thực phẩm và đánh giá tính hợp lệ của mô hình hành vi Andersen để dự đoán việc sử dụng tem phiếu thực phẩm của những người lớn tuổi nghèo khó. THIẾT KẾ VÀ PHƯƠNG PHÁP Chúng tôi đã tiến hành phân tích thứ cấp Khảo sát cộng đồng Hoa Kỳ năm 2003, đây là một cuộc khảo sát đại diện toàn quốc với tỷ lệ phản hồi là 96,7%. Chúng tôi hạn chế mẫu nghiên cứu của mình đối với 14.724 công dân Hoa Kỳ nghèo khó từ 65 tuổi trở lên. Chúng tôi đã sử dụng phân tích hồi quy nhị biến và hồi quy logistic để so sánh 2.796 người nhận tem phiếu thực phẩm với 11.928 người không nhận. KẾT QUẢ Một trong năm công dân Mỹ cao tuổi nghèo khó đã nhận được tem phiếu thực phẩm trong năm trước. Những người trả lời là nữ, người thuê nhà, người trả lời trẻ hơn, người khuyết tật và những người nhận Thu nhập an sinh bổ sung hoặc phúc lợi có nhiều khả năng nhận được tem phiếu thực phẩm hơn. Giá trị pseudo-R-square cho thấy Mô hình hành vi Andersen giải thích được 28% biến động của mô hình. Ý NGHĨA Cần cải thiện việc nhắm mục tiêu để tăng tỷ lệ tham gia của người lớn tuổi. Các văn phòng tem phiếu thực phẩm lưu động và vệ tinh ở các khu phố có thu nhập thấp và các chương trình tiếp cận sáng tạo khác hợp tác với các đối tác cộng đồng cũng có thể cải thiện khả năng tiếp cận. Với phần lớn người lớn tuổi nghèo khó không nhận được tem phiếu thực phẩm, các chiến lược như thế này cực kỳ quan trọng để khắc phục tình trạng này trong nhóm người Mỹ cao tuổi dễ bị tổn thương nhất."} +{"idx": 1960031, "en": "PURPOSE The purpose of this study was to identify relationships between medical, functional, economic, oral health, social, religious, and psychological factors and under-eating in homebound older adults. The focus of the study was on identifying potentially modifiable factors amenable to social and behavioral interventions. DESIGN AND METHODS A total of 230 homebound older adults who were currently receiving home health services participated in interviews in their homes using a questionnaire to assess eating behaviors and factors that could possibly affect those eating behaviors. Interviewers measured height and weight, and participants completed three 24-hr dietary recalls. RESULTS The mean age of participants was 79.1 years. The sample comprised 78% women and 38% African Americans. We found that 70% of participants were under-eating, defined as not consuming enough calories to maintain their current body weight. Participants who were at higher risk of under-eating included men, those receiving either infrequent care or very frequent care by a caregiver, those who had been hospitalized prior to receipt of home health services, and those with a higher body mass index. IMPLICATIONS Findings from the study have implications for both practice and policy. Experts must develop evidence-based interventions targeted at under-eating in this particularly vulnerable and growing population of homebound older adults. This study provides an initial foundation for the development of targeted evidence-based behavioral nutritional interventions that are noninvasive and cost effective.", "gemini": "MỤC ĐÍCH Mục đích của nghiên cứu này là xác định mối quan hệ giữa các yếu tố y tế, chức năng, kinh tế, sức khỏe răng miệng, xã hội, tôn giáo và tâm lý với tình trạng ăn uống không đủ ở những người lớn tuổi tại nhà. Trọng tâm của nghiên cứu là xác định các yếu tố có khả năng thay đổi có thể chấp nhận được đối với các biện pháp can thiệp xã hội và hành vi. THIẾT KẾ VÀ PHƯƠNG PHÁP Tổng cộng 230 người lớn tuổi tại nhà hiện đang nhận dịch vụ chăm sóc sức khỏe tại nhà đã tham gia phỏng vấn tại nhà của họ bằng cách sử dụng bảng câu hỏi để đánh giá hành vi ăn uống và các yếu tố có thể ảnh hưởng đến các hành vi ăn uống đó. Người phỏng vấn đã đo chiều cao và cân nặng, và những người tham gia đã hoàn thành ba lần thu hồi chế độ ăn uống trong 24 giờ. KẾT QUẢ Độ tuổi trung bình của những người tham gia là 79,1 tuổi. Mẫu bao gồm 78% phụ nữ và 38% người Mỹ gốc Phi. Chúng tôi thấy rằng 70% những người tham gia đang ăn uống không đủ, được định nghĩa là không tiêu thụ đủ calo để duy trì cân nặng hiện tại của họ. Những người tham gia có nguy cơ ăn uống không đủ cao hơn bao gồm nam giới, những người nhận được sự chăm sóc không thường xuyên hoặc chăm sóc rất thường xuyên của người chăm sóc, những người đã nhập viện trước khi nhận được dịch vụ chăm sóc sức khỏe tại nhà và những người có chỉ số khối cơ thể cao hơn. Ý NGHĨA Những phát hiện từ nghiên cứu có ý nghĩa đối với cả hoạt động thực hành và chính sách. Các chuyên gia phải phát triển các biện pháp can thiệp dựa trên bằng chứng nhắm vào tình trạng ăn uống không đủ ở nhóm người lớn tuổi tại nhà dễ bị tổn thương và đang gia tăng này. Nghiên cứu này cung cấp nền tảng ban đầu cho việc phát triển các biện pháp can thiệp dinh dưỡng hành vi dựa trên bằng chứng có mục tiêu, không xâm lấn và hiệu quả về chi phí."} +{"idx": 1960032, "en": "PURPOSE We sought to determine how women aged 80 years or older value different preventive health measures compared to women aged 65 to 79 years. DESIGN AND METHODS We surveyed 107 women aged 80 years or older and 93 women aged 65 to 79 years; we randomly selected all of them from a large academic primary care practice. We measured perceived importance and priority placed on different preventive health measures, including screening tests; counseling on healthy lifestyle and geriatric health issues; immunizations; and recommendations for over-the-counter prevention medications. RESULTS Of the 200 women, 28.5% were aged 80 to 84 and 25.0% were aged 85 years or older. The majority of the women were non-Hispanic White (65.5%), had private insurance (82.0%), and were in good health condition (52.0%). Women aged between 65 and 79 were more likely than women aged 80 or older to consider screening tests and exercise counseling essential or very important to maintaining their health. Women aged 80 or older did not value any preventive health measure more highly than did younger women. Women who were 65 to 79 years of age ranked mammography screening as their most valued preventive health measure, with five of their top six measures being screening tests. Women who were 85 years of age or older prioritized flu shots, recommendations for aspirin, and then mammography screening. IMPLICATIONS Screening tests and exercise counseling are more highly valued by women aged 65 to 79 years than by women aged 80 years or older. Regardless of age, mammography screening is prioritized over other preventive health measures. Understanding how older women value different preventive health measures may help clinicians improve their preventive health counseling.", "gemini": "MỤC ĐÍCH Chúng tôi tìm cách xác định phụ nữ từ 80 tuổi trở lên đánh giá các biện pháp phòng ngừa sức khỏe khác nhau như thế nào so với phụ nữ từ 65 đến 79 tuổi. THIẾT KẾ VÀ PHƯƠNG PHÁP Chúng tôi đã khảo sát 107 phụ nữ từ 80 tuổi trở lên và 93 phụ nữ từ 65 đến 79 tuổi; chúng tôi đã chọn ngẫu nhiên tất cả họ từ một cơ sở chăm sóc chính học thuật lớn. Chúng tôi đo lường mức độ quan trọng được nhận thức và ưu tiên đặt vào các biện pháp phòng ngừa sức khỏe khác nhau, bao gồm các xét nghiệm sàng lọc; tư vấn về lối sống lành mạnh và các vấn đề sức khỏe tuổi già; tiêm chủng; và các khuyến nghị về thuốc phòng ngừa không kê đơn. KẾT QUẢ Trong số 200 phụ nữ, 28,5% từ 80 đến 84 tuổi và 25,0% từ 85 tuổi trở lên. Phần lớn phụ nữ là người da trắng không phải gốc Tây Ban Nha (65,5%), có bảo hiểm tư nhân (82,0%) và có tình trạng sức khỏe tốt (52,0%). Phụ nữ từ 65 đến 79 tuổi có nhiều khả năng hơn phụ nữ từ 80 tuổi trở lên coi các xét nghiệm sàng lọc và tư vấn tập thể dục là thiết yếu hoặc rất quan trọng để duy trì sức khỏe của họ. Phụ nữ từ 80 tuổi trở lên không đánh giá cao bất kỳ biện pháp phòng ngừa sức khỏe nào hơn phụ nữ trẻ hơn. Những phụ nữ từ 65 đến 79 tuổi xếp hạng chụp nhũ ảnh là biện pháp phòng ngừa sức khỏe mà họ đánh giá cao nhất, với năm trong sáu biện pháp hàng đầu của họ là xét nghiệm sàng lọc. Những phụ nữ từ 85 tuổi trở lên ưu tiên tiêm phòng cúm, khuyến cáo dùng aspirin, sau đó là chụp nhũ ảnh. Ý NGHĨA Các xét nghiệm sàng lọc và tư vấn tập thể dục được phụ nữ từ 65 đến 79 tuổi đánh giá cao hơn so với phụ nữ từ 80 tuổi trở lên. Bất kể tuổi tác, chụp nhũ ảnh được ưu tiên hơn các biện pháp phòng ngừa sức khỏe khác. Hiểu được cách phụ nữ lớn tuổi đánh giá các biện pháp phòng ngừa sức khỏe khác nhau có thể giúp các bác sĩ lâm sàng cải thiện tư vấn phòng ngừa sức khỏe của họ."} +{"idx": 1960033, "en": "PURPOSE Despite the documented benefits of physical activity, it remains difficult to motivate older adults to start and maintain regular physical activity. This study tested an innovative intervention for mobilizing older adults into a neighborhood-based walking program. DESIGN AND METHODS Researchers recruited a total of 260 healthy but insufficiently active adults aged 65 to 74 years and randomly selected from the Australian electoral roll from 30 Perth metropolitan neighborhoods. Social cognitive theory guided the design of the program. Researchers collected both qualitative and quantitative data to inform the development, together with ongoing process evaluation. RESULTS A total of 65% of participants completed the program. Their mean weekly walking time for recreation increased by about 100 min, and 80% of participants reported that they would continue to walk twice per week upon program completion. IMPLICATIONS This practical program is potentially effective and sustainable with respect to mobilizing physically inactive older people.", "gemini": "MỤC ĐÍCH Mặc dù các lợi ích của hoạt động thể chất đã được chứng minh, nhưng vẫn khó để thúc đẩy người cao tuổi bắt đầu và duy trì hoạt động thể chất thường xuyên. Nghiên cứu này đã thử nghiệm một biện pháp can thiệp sáng tạo để huy động người cao tuổi tham gia chương trình đi bộ tại khu phố. THIẾT KẾ VÀ PHƯƠNG PHÁP Các nhà nghiên cứu đã tuyển dụng tổng cộng 260 người lớn khỏe mạnh nhưng ít vận động ở độ tuổi từ 65 đến 74 và được chọn ngẫu nhiên từ danh sách cử tri của Úc từ 30 khu vực đô thị Perth. Lý thuyết nhận thức xã hội hướng dẫn thiết kế chương trình. Các nhà nghiên cứu đã thu thập cả dữ liệu định tính và định lượng để cung cấp thông tin cho quá trình phát triển, cùng với quá trình đánh giá liên tục. KẾT QUẢ Tổng cộng 65% người tham gia đã hoàn thành chương trình. Thời gian đi bộ trung bình hàng tuần của họ để giải trí tăng khoảng 100 phút và 80% người tham gia báo cáo rằng họ sẽ tiếp tục đi bộ hai lần một tuần sau khi hoàn thành chương trình. Ý NGHĨA Chương trình thực tế này có khả năng hiệu quả và bền vững đối với việc huy động những người cao tuổi ít vận động."} +{"idx": 1960034, "en": "Most people with arthritis are not regularly active. Understanding what factors influence exercise is essential for designing programs to increase participation. The objective of this study was to examine the correlates of exercise in people with arthritis. Using a cross-sectional design, sociodemographic, health-related, and psychosocial variables were collected from community-dwelling individuals with arthritis (N = 141). Associations with exercise level were examined with bivariate statistics (ANOVAs, chi-squares) and logistic-regression analyses. Exercisers were less likely than nonexercisers and insufficiently active people to report that arthritis negatively affected their physical and social functioning, and they reported more positive affect and greater self-efficacy (p < .05). Exercisers also reported less pain than nonexercisers (p < .05). In multiple logistic-regression analyses, self-efficacy and physical limitations remained independent predictors of exercise. The results suggest the need to target exercise self-efficacy when designing exercise interventions. Results also suggest the need to tailor exercise programs to individuals' physical limitations.", "gemini": "Hầu hết những người bị viêm khớp đều không hoạt động thường xuyên. Việc hiểu được những yếu tố nào ảnh hưởng đến việc tập thể dục là rất cần thiết để thiết kế các chương trình nhằm tăng cường sự tham gia. Mục tiêu của nghiên cứu này là kiểm tra mối tương quan của việc tập thể dục ở những người bị viêm khớp. Sử dụng thiết kế cắt ngang, các biến nhân khẩu học, sức khỏe và tâm lý xã hội được thu thập từ những người mắc bệnh viêm khớp sống trong cộng đồng (N = 141). Các mối liên quan với mức độ tập thể dục đã được kiểm tra bằng số liệu thống kê hai biến (ANOVA, chi bình phương) và phân tích hồi quy logistic. Những người tập thể dục ít có khả năng báo cáo rằng viêm khớp ảnh hưởng tiêu cực đến chức năng thể chất và xã hội của họ hơn những người không tập thể dục và những người ít vận động, và họ báo cáo rằng tình cảm tích cực hơn và hiệu quả bản thân cao hơn (p < .05). Những người tập thể dục cũng báo cáo rằng họ ít đau hơn những người không tập thể dục (p < .05). Trong nhiều phân tích hồi quy logistic, hiệu quả bản thân và hạn chế về thể chất vẫn là những yếu tố dự báo độc lập về việc tập thể dục. Kết quả cho thấy cần phải nhắm mục tiêu vào hiệu quả bản thân khi thiết kế các biện pháp can thiệp tập thể dục. Kết quả cũng cho thấy cần phải điều chỉnh các chương trình tập thể dục theo những hạn chế về thể chất của từng cá nhân."} +{"idx": 1960035, "en": "This study examined whether features of the built environment and residents' perceptions of neighborhood walkability, safety, and social cohesion were associated with self-reported physical activity (PA) and community-based activity among a sample of 190 older adults (mean age 74) residing in 8 Denver neighborhoods. Neighborhood walking audits were conducted to assess walkability and social capital. In multilevel analyses, a few walkability variables including curb cuts, crosswalks, and density of retail predicted greater frequency of walking for errands (p < .05), but mean frequency of walking for errands for this sample was low (<1/wk). Contrary to expectations, total PA and community-based activity were highest in neighborhoods with fewer walkability variables but higher respondent perceptions of safety and social cohesion (p < .01). For seniors, the importance of characteristics of the built environment in promoting PA and general activity engagement might be secondary to attributes of the social environment that promote safety and social cohesion.", "gemini": "Nghiên cứu này đã kiểm tra xem các đặc điểm của môi trường được xây dựng và nhận thức của cư dân về khả năng đi bộ, sự an toàn và sự gắn kết xã hội của khu phố có liên quan đến hoạt động thể chất tự báo cáo (PA) và hoạt động dựa vào cộng đồng trong số 190 người cao tuổi (tuổi trung bình 74) cư trú tại 8 khu phố Denver hay không. Các cuộc kiểm toán đi bộ tại khu phố đã được tiến hành để đánh giá khả năng đi bộ và vốn xã hội. Trong các phân tích đa cấp, một số biến số về khả năng đi bộ bao gồm cắt lề đường, vạch qua đường và mật độ bán lẻ dự đoán tần suất đi bộ để làm việc vặt cao hơn (p < .05), nhưng tần suất đi bộ trung bình để làm việc vặt đối với mẫu này là thấp (<1/tuần). Trái với mong đợi, tổng PA và hoạt động dựa vào cộng đồng là cao nhất ở những khu phố có ít biến số về khả năng đi bộ nhưng người trả lời nhận thức cao hơn về sự an toàn và gắn kết xã hội (p < .01). Đối với người cao tuổi, tầm quan trọng của các đặc điểm của môi trường được xây dựng trong việc thúc đẩy PA và sự tham gia hoạt động chung có thể là thứ yếu so với các thuộc tính của môi trường xã hội thúc đẩy sự an toàn và gắn kết xã hội."} +{"idx": 1960036, "en": "The aim of this study was to analyze differences in biomechanical parameters between elderly and control participants when stepping up, to evaluate control of balance. Eleven control and 14 elderly participants performed a step from an initial static posture onto a 7-cm-high force plate. For the spontaneous-velocity condition, elderly participants performed a slower progression velocity than control participants. Elderly participants spent proportionally more time in stance phase, with a corresponding decrease in swing phase, than the control participants, irrespective of movement velocity. In contrast, at spontaneous velocity the parameters related to ground-reaction force (GRF) showed that anteroposterior and mediolateral forces at toe-off of the support limb and the slope of vertical force during weight transfer were significantly smaller for the elderly than for control participants. These GRF parameters depended on the stepping-up velocity. The elderly develop a spatiotemporal strategy and reduced movement velocity to control support balance.", "gemini": "Mục đích của nghiên cứu này là phân tích sự khác biệt về các thông số cơ sinh học giữa những người cao tuổi và những người tham gia nhóm đối chứng khi bước lên, để đánh giá khả năng kiểm soát thăng bằng. Mười một người tham gia nhóm đối chứng và 14 người cao tuổi đã thực hiện một bước từ tư thế tĩnh ban đầu lên một tấm lực kế cao 7 cm. Đối với điều kiện vận tốc tự phát, những người cao tuổi thực hiện vận tốc tiến triển chậm hơn những người tham gia nhóm đối chứng. Những người cao tuổi dành nhiều thời gian hơn theo tỷ lệ ở giai đoạn đứng, với sự giảm tương ứng ở giai đoạn vung, so với những người tham gia nhóm đối chứng, bất kể vận tốc chuyển động. Ngược lại, ở vận tốc tự phát, các thông số liên quan đến lực phản ứng mặt đất (GRF) cho thấy rằng các lực trước sau và lực giữa trong ngoài ở ngón chân của chi hỗ trợ và độ dốc của lực thẳng đứng trong quá trình truyền trọng lượng nhỏ hơn đáng kể đối với những người cao tuổi so với những người tham gia nhóm đối chứng. Các thông số GRF này phụ thuộc vào vận tốc bước lên. Người cao tuổi phát triển một chiến lược không gian thời gian và giảm vận tốc chuyển động để kiểm soát sự cân bằng hỗ trợ."} +{"idx": 1960037, "en": "The purpose of this study was to investigate the combined impact of obesity and physical activity (PA) on the health of older adults. Pedometer-determined steps/d, body-mass index (BMI), resting blood pressure, and fasting glucose (FG) were assessed in 137 older adults (69.0 +/- 8.9 yr). The active group (>4,227 steps/d) had lower systolic blood pressure (SBP; p = .001), diastolic blood pressure (DBP; p = .028), and FG (p < .001) than the inactive group (< or = 4,227 steps/d). The normal-BMI group (18.5-24.9 kg/m2) had lower SBP (p < .001) and DBP (p = .01) than the obese group (> or =30 kg/m2). There were no differences in SBP (p = .963) or DBP (p = 1.0) between active obese and inactive normal-BMI groups. The active obese group, however, had a more favorable FG than the inactive normal-BMI group (chi 2 = 18.9, df = 3, p = .001). Efforts to increase PA of older adults should receive the same priority as reducing obesity to improve BP and FG levels.", "gemini": "Mục đích của nghiên cứu này là điều tra tác động kết hợp của béo phì và hoạt động thể chất (PA) đối với sức khỏe của người cao tuổi. Các bước được xác định bằng máy đếm bước/ngày, chỉ số khối cơ thể (BMI), huyết áp nghỉ và glucose lúc đói (FG) đã được đánh giá ở 137 người cao tuổi (69,0 +/- 8,9 tuổi). Nhóm hoạt động (> 4.227 bước/ngày) có huyết áp tâm thu (SBP; p = 0,001), huyết áp tâm trương (DBP; p = 0,028) và FG (p <0,001) thấp hơn so với nhóm không hoạt động (<= 4.227 bước/ngày). Nhóm BMI bình thường (18,5-24,9 kg/m2) có SBP (p <0,001) và DBP (p = 0,01) thấp hơn so với nhóm béo phì (> hoặc = 30 kg/m2). Không có sự khác biệt về SBP (p = 0,963) hoặc DBP (p = 1,0) giữa nhóm béo phì hoạt động và nhóm BMI bình thường không hoạt động. Tuy nhiên, nhóm béo phì hoạt động có FG thuận lợi hơn so với nhóm BMI bình thường không hoạt động (chi 2 = 18,9, df = 3, p = 0,001). Những nỗ lực tăng cường PA của người cao tuổi nên được ưu tiên ngang bằng với việc giảm béo phì để cải thiện mức BP và FG."} +{"idx": 1960038, "en": "The primary purpose of this study was to investigate the accuracy of the activPAL physical activity monitor in measuring step number and cadence in older adults. Two pedometers (New-Lifestyles Digi-Walker SW-200 and New-Lifestyles NL2000) used in clinical practice to count steps were simultaneously evaluated. Observation was the criterion measure. Twenty-one participants (65-87 yr old) recruited from community-based exercise classes walked on a treadmill at 5 speeds (0.67, 0.90, 1.12, 1.33, and 1.56 m/s) and outdoors at 3 self-selected speeds (slow, normal, and fast). The absolute percentage error of the activPAL was <1% for all treadmill and outdoor conditions for measuring steps and cadence. With the exception of the slowest treadmill speed, the NL-2000 error was <2%. The SW-200 was the least accurate device, particularly at slower walking speeds. The activPAL monitor accurately recorded step number and cadence. Combined with its ability to identify primary postures, the activPAL might be a useful and versatile device for measuring activity in older adults.", "gemini": "Mục đích chính của nghiên cứu này là điều tra độ chính xác của máy theo dõi hoạt động thể chất activPAL trong việc đo số bước và nhịp độ ở người cao tuổi. Hai máy đếm bước (New-Lifestyles Digi-Walker SW-200 và New-Lifestyles NL2000) được sử dụng trong thực hành lâm sàng để đếm bước đã được đánh giá đồng thời. Quan sát là biện pháp tiêu chuẩn. Hai mươi mốt người tham gia (65-87 tuổi) được tuyển dụng từ các lớp tập thể dục dựa vào cộng đồng đã đi bộ trên máy chạy bộ với 5 tốc độ (0,67, 0,90, 1,12, 1,33 và 1,56 m/giây) và ngoài trời với 3 tốc độ tự chọn (chậm, bình thường và nhanh). Sai số phần trăm tuyệt đối của activPAL là <1% đối với tất cả các điều kiện trên máy chạy bộ và ngoài trời để đo bước và nhịp độ. Ngoại trừ tốc độ máy chạy bộ chậm nhất, sai số của NL-2000 là <2%. SW-200 là thiết bị kém chính xác nhất, đặc biệt là ở tốc độ đi bộ chậm hơn. Máy theo dõi activPAL ghi lại chính xác số bước và nhịp độ. Kết hợp với khả năng xác định tư thế chính, activPAL có thể là một thiết bị hữu ích và linh hoạt để đo hoạt động ở người cao tuổi."} +{"idx": 1960039, "en": "The Exercise Assessment and Screening for You (EASY) is a tool developed to help older individuals, their health care providers, and exercise professionals identify different types of exercise and physical activity regimens that can be tailored to meet the existing health conditions, illnesses, or disabilities of older adults. The EASY tool includes 6 screening questions that were developed based on an expert roundtable and follow-up panel activities. The philosophy behind the EASY is that screening should be a dynamic process in which participants learn to appreciate the importance of engaging in regular exercise, attending to health changes, recognizing a full range of signs and symptoms that might indicate potentially harmful events, and becoming familiar with simple safety tips for initiating and progressively increasing physical activity patterns. Representing a paradigm shift from traditional screening approaches that focus on potential risks of exercising, this tool emphasizes the benefits of exercise and physical activity for all individuals.", "gemini": "Đánh giá và sàng lọc tập thể dục dành cho bạn (EASY) là một công cụ được phát triển để giúp những người lớn tuổi, nhà cung cấp dịch vụ chăm sóc sức khỏe và các chuyên gia tập thể dục của họ xác định các loại bài tập và chế độ hoạt động thể chất khác nhau có thể được điều chỉnh để đáp ứng các tình trạng sức khỏe hiện tại, bệnh tật hoặc khuyết tật của người lớn tuổi. Công cụ EASY bao gồm 6 câu hỏi sàng lọc được phát triển dựa trên hoạt động hội thảo chuyên gia và hoạt động nhóm tiếp theo. Triết lý đằng sau EASY là sàng lọc nên là một quá trình năng động trong đó những người tham gia học cách đánh giá cao tầm quan trọng của việc tham gia tập thể dục thường xuyên, chú ý đến những thay đổi về sức khỏe, nhận ra đầy đủ các dấu hiệu và triệu chứng có thể chỉ ra các sự kiện có khả năng gây hại và trở nên quen thuộc với các mẹo an toàn đơn giản để bắt đầu và tăng dần các kiểu hoạt động thể chất. Đại diện cho sự thay đổi mô hình từ các phương pháp sàng lọc truyền thống tập trung vào các rủi ro tiềm ẩn của việc tập thể dục, công cụ này nhấn mạnh những lợi ích của việc tập thể dục và hoạt động thể chất đối với tất cả các cá nhân."} +{"idx": 1960040, "en": "Fatigue affects the capacity of muscles to generate forces and is associated with characteristic changes in EMG signals. It may also influence interjoint and intermuscular coordination. To understand better the global effects of fatigue on multijoint movement, we studied movement kinematics and EMG changes in healthy volunteers asked to hammer repetitively. Movement kinematics and the activity of 20 muscles of the arm, trunk, and leg were recorded before and after subjects became fatigued (as measured using a Borg scale). When fatigue was reached, maximal grip strength and elbow range of motion decreased while the EMG amplitude of the contralateral external oblique muscle was increased. Fatigue did not affect shoulder and wrist kinematics or movement frequency. Results suggest that fatigue influences motion at both local and global levels. Specifically, interjoint and intermuscular coordination adapt to compensate for local effects of fatigue and to maintain key movement characteristics, such as the trajectory of the end effector and the movement frequency. Nonlocal compensations may be a focus of future studies of how fatigue affects complex movements such as those typically performed in the workplace.", "gemini": "Mệt mỏi ảnh hưởng đến khả năng tạo ra lực của cơ và có liên quan đến những thay đổi đặc trưng trong các tín hiệu EMG. Nó cũng có thể ảnh hưởng đến sự phối hợp giữa các khớp và giữa các cơ. Để hiểu rõ hơn về những tác động toàn cầu của mệt mỏi đối với chuyển động của nhiều khớp, chúng tôi đã nghiên cứu về động học chuyển động và những thay đổi EMG ở những tình nguyện viên khỏe mạnh được yêu cầu đập búa liên tục. Động học chuyển động và hoạt động của 20 cơ ở cánh tay, thân và chân đã được ghi lại trước và sau khi các đối tượng trở nên mệt mỏi (được đo bằng thang Borg). Khi đạt đến trạng thái mệt mỏi, sức nắm tối đa và phạm vi chuyển động của khuỷu tay giảm trong khi biên độ EMG của cơ liên sườn ngoài đối bên tăng lên. Mệt mỏi không ảnh hưởng đến động học của vai và cổ tay hoặc tần suất chuyển động. Kết quả cho thấy mệt mỏi ảnh hưởng đến chuyển động ở cả cấp độ cục bộ và toàn cầu. Cụ thể, sự phối hợp giữa các khớp và giữa các cơ thích nghi để bù đắp cho các tác động cục bộ của mệt mỏi và để duy trì các đặc điểm chuyển động chính, chẳng hạn như quỹ đạo của bộ phận cuối và tần suất chuyển động. Sự bù trừ không cục bộ có thể là trọng tâm của các nghiên cứu trong tương lai về cách mệt mỏi ảnh hưởng đến các chuyển động phức tạp như những chuyển động thường được thực hiện tại nơi làm việc."} +{"idx": 1960041, "en": "Individuals with multiple sclerosis (MS) often have poor balance control that is especially apparent during dynamic tasks such as gait initiation (GI). The purpose of this study was to investigate how balance symptoms due to MS alter spatiotemporal variables, coordination, and temporal margins within the stability boundary during gait initiation. Twelve women with MS (Expanded Disability Status Scale [EDSS] mean = 4.0, SD = 1.4) and 12 women without MS (control group) initiated gait at their preferred speed. MS participants attained a slower anterior velocity because of smaller anterior center of mass displacements and took longer to complete the initiation of gait than the control group. MS participants exhibited a smaller posterior shift in center of pressure during GI and stepped with a longer dual support time than the control group. However, these changes may be due to differences in initiation velocity. Relative timing analysis showed invariance in postural and locomotor phases of gait initiation between groups. The MS group showed different coordination between anterior-posterior and medio-lateral center of pressure components while increasing temporal margins to the posterior and lateral stability boundaries in comparison with the control group. Overall, during gait initiation at their preferred speed the MS participants adopted a functional strategy that produces lower speed and reduced proximity to the stability boundaries prior to stepping.", "gemini": "Những người mắc bệnh đa xơ cứng (MS) thường có khả năng kiểm soát thăng bằng kém, điều này đặc biệt rõ ràng trong các nhiệm vụ động như bắt đầu đi bộ (GI). Mục đích của nghiên cứu này là để điều tra xem các triệu chứng mất cân bằng do MS làm thay đổi các biến số không gian-thời gian, sự phối hợp và biên độ thời gian trong ranh giới ổn định như thế nào trong quá trình bắt đầu đi bộ. Mười hai phụ nữ mắc MS (Thang trạng thái khuyết tật mở rộng [EDSS] trung bình = 4,0, SD = 1,4) và 12 phụ nữ không mắc MS (nhóm đối chứng) bắt đầu đi bộ với tốc độ ưa thích của họ. Những người tham gia MS đạt được vận tốc trước chậm hơn do dịch chuyển trọng tâm trước nhỏ hơn và mất nhiều thời gian hơn để hoàn thành việc bắt đầu đi bộ so với nhóm đối chứng. Những người tham gia MS biểu hiện sự dịch chuyển về phía sau nhỏ hơn ở trọng tâm trong quá trình GI và bước với thời gian hỗ trợ kép dài hơn so với nhóm đối chứng. Tuy nhiên, những thay đổi này có thể là do sự khác biệt về vận tốc khởi đầu. Phân tích thời gian tương đối cho thấy sự bất biến ở các giai đoạn tư thế và vận động của quá trình bắt đầu đi bộ giữa các nhóm. Nhóm MS cho thấy sự phối hợp khác nhau giữa các thành phần trọng tâm trước-sau và giữa-ngoài trong khi tăng biên độ thời gian đến các ranh giới ổn định phía sau và bên so với nhóm đối chứng. Nhìn chung, trong quá trình bắt đầu đi bộ với tốc độ ưa thích của họ, những người tham gia MS đã áp dụng một chiến lược chức năng tạo ra tốc độ thấp hơn và giảm khoảng cách đến các ranh giới ổn định trước khi bước."} +{"idx": 1960042, "en": "The execution of a fast aimed movement takes less time when it is performed in isolation than when it is followed by a movement to a second target. The most recent explanation of this so-called one-target advantage (OTA) is the movement integration hypothesis. The first movement is slowed down to enable a neuromuscular integration of the first and second movements so that a presumably smooth transition between the two movements can take place. The present study shows that the time increase underlying the OTA does not happen just before the transition takes place, but is located in the initial part of the first movement element of the two-tap sequence. It is discussed how such a temporal occurrence relates to the transition of the first movement to the second.", "gemini": "Việc thực hiện một chuyển động nhanh có mục tiêu mất ít thời gian hơn khi nó được thực hiện một cách đơn lẻ so với khi nó được theo sau bởi một chuyển động đến một mục tiêu thứ hai. Giải thích gần đây nhất về cái gọi là lợi thế một mục tiêu (OTA) này là giả thuyết tích hợp chuyển động. Chuyển động đầu tiên bị chậm lại để cho phép tích hợp thần kinh cơ của chuyển động đầu tiên và thứ hai để có thể diễn ra quá trình chuyển đổi trơn tru giữa hai chuyển động. Nghiên cứu hiện tại cho thấy sự gia tăng thời gian cơ bản của OTA không chỉ xảy ra ngay trước khi quá trình chuyển đổi diễn ra, mà nằm ở phần đầu của yếu tố chuyển động đầu tiên của chuỗi hai lần chạm. Người ta đã thảo luận về cách thức xảy ra tạm thời như vậy liên quan đến quá trình chuyển đổi của chuyển động đầu tiên sang chuyển động thứ hai."} +{"idx": 1960043, "en": "This study evaluated the effect of context on the reaching performance of the unaffected arm and postural control while standing in patients with right cerebral vascular accidents (RCVA) and in healthy adults. Fifteen subjects with RCVA and sixteen healthy subjects performed tasks with the right hand under two conditions while standing. One condition involved moving coins forward on a table as far as possible (concrete task) and the other reaching forward without a target (abstract task). Forward reaching distance, forward displacement and lateral shift of center of pressure (CoP), and weight distribution were the dependent variables. The RCVA and control groups achieved significantly greater reaching distances in the concrete task than in the abstract one. The RCVA group showed significantly less lateral shift of the CoP and placed more weight on the affected leg in the concrete than the abstract task, whereas the control group made a greater lateral shift in the concrete task and had a similar mean ratio of weight distribution during both tasks. The results demonstrate that a functional application of task targets may favorably modulate both reaching and posture performance and exert various positive affects on postural control. Such applications may have a place in the therapeutic recovery efforts for patients afflicted with stroke.", "gemini": "Nghiên cứu này đánh giá tác động của bối cảnh đến hiệu suất với tới của cánh tay không bị ảnh hưởng và kiểm soát tư thế khi đứng ở những bệnh nhân bị tai biến mạch máu não phải (RCVA) và ở người lớn khỏe mạnh. Mười lăm đối tượng mắc RCVA và mười sáu đối tượng khỏe mạnh đã thực hiện các nhiệm vụ bằng tay phải trong hai điều kiện khi đứng. Một điều kiện liên quan đến việc di chuyển các đồng xu về phía trước trên bàn càng xa càng tốt (nhiệm vụ cụ thể) và nhiệm vụ còn lại là với tới phía trước mà không có mục tiêu (nhiệm vụ trừu tượng). Khoảng cách với tới về phía trước, độ dịch chuyển về phía trước và độ dịch chuyển bên của trọng tâm (CoP) và phân bố trọng lượng là các biến phụ thuộc. Các nhóm RCVA và nhóm đối chứng đạt được khoảng cách với tới lớn hơn đáng kể trong nhiệm vụ cụ thể so với nhiệm vụ trừu tượng. Nhóm RCVA cho thấy độ dịch chuyển bên của CoP ít hơn đáng kể và đặt nhiều trọng lượng hơn lên chân bị ảnh hưởng trong nhiệm vụ cụ thể so với nhiệm vụ trừu tượng, trong khi nhóm đối chứng thực hiện độ dịch chuyển bên lớn hơn trong nhiệm vụ cụ thể và có tỷ lệ phân bố trọng lượng trung bình tương tự trong cả hai nhiệm vụ. Kết quả chứng minh rằng việc áp dụng chức năng của các mục tiêu nhiệm vụ có thể điều chỉnh thuận lợi cả hiệu suất với tới và tư thế và tạo ra nhiều ảnh hưởng tích cực khác nhau đến kiểm soát tư thế. Các ứng dụng như vậy có thể có vị trí trong các nỗ lực phục hồi điều trị cho những bệnh nhân bị đột quỵ."} +{"idx": 1960044, "en": "To assess the postural strategies developed by patients after total hip arthroplasty (THA), 14 patients were measured 12 days after surgery. The respective role played by both sound and prosthetic legs and the compensatory mechanisms were assessed through a separate measure of the center-of-pressure (CP) trajectories under each foot. The movements of the center-of-gravity (CG) were estimated from those of the resultant CP to determine postural performance. The postural behavior was compared with those of a group of age-matched healthy subjects required to adopt a slightly asymmetrical weight distribution. Patient results indicate greater movements for both plantar and resultant CP displacements, principally along the antero-posterior (AP) axis, a decreased contribution of the hip mechanisms in the production of CP displacements along the medio-lateral (ML) axis, greater resultant CP and CG movements along the AP axis and increased differences between CP and CG along both ML and AP axes. The postural specificity of the THA patients appears to be due to a global sensorimotor impairment that alters the control of the loading-unloading mechanism at the hip level.", "gemini": "Để đánh giá các chiến lược về tư thế do bệnh nhân phát triển sau khi thay khớp háng toàn phần (THA), 14 bệnh nhân đã được đo sau 12 ngày phẫu thuật. Vai trò tương ứng của cả chân lành và chân giả và các cơ chế bù trừ đã được đánh giá thông qua một biện pháp riêng biệt về các quỹ đạo trọng tâm (CP) dưới mỗi bàn chân. Các chuyển động của trọng tâm (CG) được ước tính từ các chuyển động của CP kết quả để xác định hiệu suất tư thế. Hành vi tư thế được so sánh với hành vi của một nhóm đối tượng khỏe mạnh có độ tuổi tương ứng được yêu cầu áp dụng phân phối trọng lượng hơi không đối xứng. Kết quả của bệnh nhân cho thấy các chuyển động lớn hơn đối với cả các dịch chuyển CP về phía gan bàn chân và CP kết quả, chủ yếu dọc theo trục trước sau (AP), sự đóng góp giảm của các cơ chế hông trong việc tạo ra các dịch chuyển CP dọc theo trục giữa bên (ML), các chuyển động CP và CG kết quả lớn hơn dọc theo trục AP và sự khác biệt gia tăng giữa CP và CG dọc theo cả trục ML và AP. Tính đặc hiệu về tư thế của bệnh nhân THA dường như là do suy giảm cảm giác vận động toàn cầu làm thay đổi cơ chế tải-dỡ ở mức hông."} +{"idx": 1960045, "en": "We explored possible effects of negative covariation among finger forces in multifinger accurate force production tasks on the classical Fitts's speed-accuracy trade-off. Healthy subjects performed cyclic force changes between pairs of targets \"as quickly and accurately as possible.\" Tasks with two force amplitudes and six ratios of force amplitude to target size were performed by each of the four fingers of the right hand and four finger combinations. There was a close to linear relation between movement time and the log-transformed ratio of target amplitude to target size across all finger combinations. There was a close to linear relation between standard deviation of force amplitude and movement time. There were no differences between the performance of either of the two \"radial\" fingers (index and middle) and the multifinger tasks. The \"ulnar\" fingers (little and ring) showed higher indices of variability and longer movement times as compared with both \"radial\" fingers and multifinger combinations. We conclude that potential effects of the negative covariation and also of the task-sharing across a set of fingers are counterbalanced by an increase in individual finger force variability in multifinger tasks as compared with single-finger tasks. The results speak in favor of a feed-forward model of multifinger synergies. They corroborate a hypothesis that multifinger synergies are created not to improve overall accuracy, but to allow the system larger flexibility, for example to deal with unexpected perturbations and concomitant tasks.", "gemini": "Chúng tôi đã khám phá những tác động có thể có của sự biến thiên nghịch giữa các lực ngón tay trong các nhiệm vụ tạo lực chính xác bằng nhiều ngón tay đối với sự đánh đổi tốc độ-độ chính xác cổ điển của Fitts. Những đối tượng khỏe mạnh đã thực hiện các thay đổi lực tuần hoàn giữa các cặp mục tiêu \"nhanh và chính xác nhất có thể\". Các nhiệm vụ với hai biên độ lực và sáu tỷ lệ biên độ lực so với kích thước mục tiêu được thực hiện bởi mỗi ngón tay trong số bốn ngón tay của bàn tay phải và bốn tổ hợp ngón tay. Có mối quan hệ gần như tuyến tính giữa thời gian chuyển động và tỷ lệ biên độ mục tiêu đã được chuyển đổi logarit so với kích thước mục tiêu trên tất cả các tổ hợp ngón tay. Có mối quan hệ gần như tuyến tính giữa độ lệch chuẩn của biên độ lực và thời gian chuyển động. Không có sự khác biệt giữa hiệu suất của bất kỳ ngón tay \"hướng tâm\" nào (trỏ và giữa) và các nhiệm vụ nhiều ngón tay. Các ngón tay \"hướng tâm\" (út và áp út) cho thấy chỉ số biến động cao hơn và thời gian chuyển động dài hơn so với cả ngón tay \"hướng tâm\" và tổ hợp nhiều ngón tay. Chúng tôi kết luận rằng các tác động tiềm ẩn của sự biến thiên nghịch và cả việc chia sẻ nhiệm vụ trên một tập hợp các ngón tay được cân bằng bởi sự gia tăng biến động lực ngón tay riêng lẻ trong các nhiệm vụ nhiều ngón tay so với các nhiệm vụ một ngón tay. Các kết quả ủng hộ một mô hình tiến triển của sự phối hợp nhiều ngón tay. Chúng xác nhận giả thuyết rằng sự phối hợp nhiều ngón tay được tạo ra không phải để cải thiện độ chính xác tổng thể mà để cho phép hệ thống linh hoạt hơn, ví dụ như để đối phó với những nhiễu động bất ngờ và các nhiệm vụ đồng thời."} +{"idx": 1960046, "en": "We describe a brain-computer interface for controlling a humanoid robot directly using brain signals obtained non-invasively from the scalp through electroencephalography (EEG). EEG has previously been used for tasks such as controlling a cursor and spelling a word, but it has been regarded as an unlikely candidate for more complex forms of control owing to its low signal-to-noise ratio. Here we show that by leveraging advances in robotics, an interface based on EEG can be used to command a partially autonomous humanoid robot to perform complex tasks such as walking to specific locations and picking up desired objects. Visual feedback from the robot's cameras allows the user to select arbitrary objects in the environment for pick-up and transport to chosen locations. Results from a study involving nine users indicate that a command for the robot can be selected from four possible choices in 5 s with 95% accuracy. Our results demonstrate that an EEG-based brain-computer interface can be used for sophisticated robotic interaction with the environment, involving not only navigation as in previous applications but also manipulation and transport of objects.", "gemini": "Chúng tôi mô tả một giao diện não-máy tính để điều khiển một rô bốt hình người trực tiếp bằng các tín hiệu não thu được không xâm lấn từ da đầu thông qua điện não đồ (EEG). EEG trước đây đã được sử dụng cho các nhiệm vụ như điều khiển con trỏ và đánh vần một từ, nhưng nó được coi là ứng cử viên không thích hợp cho các hình thức điều khiển phức tạp hơn do tỷ lệ tín hiệu trên nhiễu thấp. Ở đây chúng tôi chỉ ra rằng bằng cách tận dụng những tiến bộ trong robot học, một giao diện dựa trên EEG có thể được sử dụng để ra lệnh cho một rô bốt hình người bán tự động thực hiện các nhiệm vụ phức tạp như đi bộ đến các địa điểm cụ thể và nhặt các vật thể mong muốn. Phản hồi hình ảnh từ camera của rô bốt cho phép người dùng chọn các vật thể tùy ý trong môi trường để nhặt và vận chuyển đến các địa điểm đã chọn. Kết quả từ một nghiên cứu liên quan đến chín người dùng chỉ ra rằng một lệnh cho rô bốt có thể được chọn từ bốn lựa chọn có thể trong 5 giây với độ chính xác 95%. Kết quả của chúng tôi chứng minh rằng một giao diện não-máy tính dựa trên EEG có thể được sử dụng để tương tác với rô bốt tinh vi với môi trường, không chỉ bao gồm điều hướng như trong các ứng dụng trước đây mà còn bao gồm cả thao tác và vận chuyển các vật thể."} +{"idx": 1960047, "en": "BACKGROUND Urinary incontinence is an annoying, uncomfortable and unpleasant condition affecting the elderly. The problem of bedwetting and other urinary complaints are common in rest homes. OBJECTIVE Our study aimed to determine the efficiency of bladder training and Kegel exercises for older women living in a rest home. METHODS This is an experimental prospective research study. Through a randomization process, 25 women were included in the treatment group, and another 25 were included in the control group. Participants were living in a rest home for women aged older than 65 years with urinary complaints. The pretreatment interview form, Quality of Life Scale, Mini-Mental Test, Rankin Scale, daily urinary forms and pad tests were administered to the treatment and control groups. Bladder training and Kegel exercises were given to the treatment group for 6-8 weeks. The second evaluation was performed 8 weeks after treatment, and the last evaluation was carried out 6 months after treatment. RESULTS The average age of the treatment group was 78.88 +/- 4.80 years, and the average age of the control group 79.44 +/- 5.32 years. Urgency, frequency and nocturia were common complaints. Pretreatment, 8-week and 6-month evaluations revealed that the amount of urinary incontinence with urgency, frequency and nocturia complaints statistically and significantly decreased in the treatment group compared to the control group. In the pad test results, a statistically significant decrease was observed in the treatment group compared to the control group. A significant increase in pelvic floor strength was observed in the treatment group compared to the control group upon all evaluations. CONCLUSION Behavioral therapy can be used easily as an effective treatment for urinary incontinence in elderly women living at a rest home.", "gemini": "BỐI CẢNH Tiểu không tự chủ là một tình trạng khó chịu, không thoải mái và khó chịu ảnh hưởng đến người cao tuổi. Vấn đề đái dầm và các vấn đề về tiết niệu khác thường gặp ở các viện dưỡng lão. MỤC TIÊU Nghiên cứu của chúng tôi nhằm xác định hiệu quả của bài tập luyện bàng quang và bài tập Kegel đối với phụ nữ lớn tuổi sống tại viện dưỡng lão. PHƯƠNG PHÁP Đây là một nghiên cứu nghiên cứu triển vọng thực nghiệm. Thông qua quá trình chọn ngẫu nhiên, 25 phụ nữ được đưa vào nhóm điều trị và 25 phụ nữ khác được đưa vào nhóm đối chứng. Những người tham gia đang sống tại viện dưỡng lão dành cho phụ nữ từ 65 tuổi trở lên có các vấn đề về tiết niệu. Biểu mẫu phỏng vấn trước khi điều trị, Thang chất lượng cuộc sống, Bài kiểm tra trí tuệ mini, Thang Rankin, biểu mẫu tiết niệu hàng ngày và bài kiểm tra miếng lót đã được thực hiện cho nhóm điều trị và nhóm đối chứng. Bài tập luyện bàng quang và bài tập Kegel đã được thực hiện cho nhóm điều trị trong 6-8 tuần. Đánh giá thứ hai được thực hiện 8 tuần sau khi điều trị và đánh giá cuối cùng được thực hiện 6 tháng sau khi điều trị. KẾT QUẢ Độ tuổi trung bình của nhóm điều trị là 78,88 +/- 4,80 tuổi và độ tuổi trung bình của nhóm đối chứng là 79,44 +/- 5,32 tuổi. Đi tiểu gấp, tiểu nhiều lần và tiểu đêm là những phàn nàn phổ biến. Đánh giá trước khi điều trị, 8 tuần và 6 tháng cho thấy lượng tiểu không tự chủ khi đi tiểu gấp, tiểu nhiều lần và tiểu đêm giảm đáng kể về mặt thống kê ở nhóm điều trị so với nhóm đối chứng. Trong kết quả kiểm tra miếng lót, có sự giảm đáng kể về mặt thống kê ở nhóm điều trị so với nhóm đối chứng. Một sự gia tăng đáng kể về sức mạnh sàn chậu đã được quan sát thấy ở nhóm điều trị so với nhóm đối chứng khi đánh giá tất cả. KẾT LUẬN Liệu pháp hành vi có thể dễ dàng được sử dụng như một phương pháp điều trị hiệu quả cho chứng tiểu không tự chủ ở phụ nữ cao tuổi sống tại viện dưỡng lão."} +{"idx": 1960048, "en": "BACKGROUND Mortality of severe sepsis and septic shock is unacceptably high. Adsorptive removal of endotoxin may interrupt the inflammatory cascade triggered by lipopolysaccharide. METHODS Prospective feasibility study with plasma separation and adsorption (PSA) treatment using a novel arginine-coated adsorber column was performed in a tertiary care gastroenterological intensive care unit. RESULTS 10 patients with severe sepsis/septic shock (median APACHE II score: 27, hospital mortality 40%) were treated with PSA on 5 consecutive days. There were no serious adverse events. No patient died during the treatment period. During treatment sessions, mean arterial pressure and cardiac power index increased while vasopressors could be reduced. Advanced oxidation protein products and in vitro pro- apoptotic activity of plasma decreased. We could not demonstrate any changes in endotoxin levels. CONCLUSIONS PSA resulted in a reduction of indicators of oxidative stress and pro-apoptotic activity of the plasma and an improvement in hemodynamic parameters, suggesting increased myocardial contractility and reduced septic vasodilation.", "gemini": "BỐI CẢNH Tỷ lệ tử vong do nhiễm trùng huyết nặng và sốc nhiễm trùng là không thể chấp nhận được. Việc loại bỏ nội độc tố bằng phương pháp hấp phụ có thể làm gián đoạn quá trình viêm do lipopolysaccharide gây ra. PHƯƠNG PHÁP Nghiên cứu khả thi có triển vọng với phương pháp điều trị tách huyết tương và hấp phụ (PSA) sử dụng một cột hấp phụ tráng arginine mới đã được thực hiện tại một khoa chăm sóc tích cực tiêu hóa bậc ba. KẾT QUẢ 10 bệnh nhân bị nhiễm trùng huyết nặng/sốc nhiễm trùng (điểm APACHE II trung bình: 27, tỷ lệ tử vong tại bệnh viện 40%) đã được điều trị bằng PSA trong 5 ngày liên tiếp. Không có tác dụng phụ nghiêm trọng nào xảy ra. Không có bệnh nhân nào tử vong trong thời gian điều trị. Trong các đợt điều trị, huyết áp động mạch trung bình và chỉ số công suất tim đã tăng trong khi có thể giảm thuốc vận mạch. Các sản phẩm oxy hóa protein nâng cao và hoạt động gây chết tế bào theo kiểu chết rụng theo chương trình trong ống nghiệm của huyết tương đã giảm. Chúng tôi không thể chứng minh bất kỳ thay đổi nào về nồng độ nội độc tố. KẾT LUẬN PSA dẫn đến việc giảm các chỉ số về stress oxy hóa và hoạt động gây chết tế bào theo kiểu chết rụng theo chương trình của huyết tương và cải thiện các thông số huyết động, cho thấy khả năng co bóp cơ tim tăng lên và giảm giãn mạch do nhiễm trùng."} +{"idx": 1960049, "en": "BACKGROUND Malnutrition is a predictor of cardiovascular disease in dialysis patients, but the mechanisms remain unknown. We investigated links between nutritional markers and arterial stiffness in continuous ambulatory peritoneal dialysis patients. METHODS We evaluated the relationship between arterial stiffness evaluated by pulse-wave velocity (PWV) and four estimates of nutritional status (serum albumin, handgrip strength [HGS], subjective global assessment [SGA], and bioelectrical impedance analysis phase angle [PA]) in 124 PD patients. RESULTS Malnourished patients exhibited a significantly higher PWV than those classified as well-nourished by SGA (p < 0.05). Furthermore, PWV correlated negatively with albumin, HGS and PA (p < 0.001, respectively). PWV was also correlated with age, systolic blood pressure, and C-reactive protein. In multivariate regression analysis, albumin, HGS, SGA and PA were each independently associated with PWV after adjustment. CONCLUSIONS The significant association between each nutritional marker and PWV in PD patients was independent of inflammation and diabetic state, suggesting that malnutrition may contribute to vascular dysfunction.", "gemini": "BỐI CẢNH Suy dinh dưỡng là một yếu tố dự báo bệnh tim mạch ở bệnh nhân chạy thận nhân tạo, nhưng cơ chế vẫn chưa được biết rõ. Chúng tôi đã nghiên cứu mối liên quan giữa các dấu hiệu dinh dưỡng và độ cứng động mạch ở những bệnh nhân chạy thận nhân tạo màng bụng liên tục. PHƯƠNG PHÁP Chúng tôi đã đánh giá mối quan hệ giữa độ cứng động mạch được đánh giá bằng vận tốc sóng xung (PWV) và bốn ước tính về tình trạng dinh dưỡng (albumin huyết thanh, sức cầm tay [HGS], đánh giá toàn cầu theo chủ quan [SGA] và góc pha phân tích trở kháng điện sinh học [PA]) ở 124 bệnh nhân PD. KẾT QUẢ Những bệnh nhân suy dinh dưỡng biểu hiện PWV cao hơn đáng kể so với những bệnh nhân được phân loại là đủ dinh dưỡng theo SGA (p < 0,05). Hơn nữa, PWV tương quan âm với albumin, HGS và PA (tương ứng là p < 0,001). PWV cũng tương quan với tuổi, huyết áp tâm thu và protein phản ứng C. Trong phân tích hồi quy đa biến, albumin, HGS, SGA và PA đều có liên quan độc lập với PWV sau khi điều chỉnh. KẾT LUẬN Mối liên hệ đáng kể giữa từng dấu hiệu dinh dưỡng và PWV ở bệnh nhân PD không phụ thuộc vào tình trạng viêm và tiểu đường, cho thấy suy dinh dưỡng có thể góp phần gây ra rối loạn chức năng mạch máu."} +{"idx": 1960050, "en": "BACKGROUND The aim of this study was to assess the effects of percutaneous transluminal angioplasty with stenting on cerebral vasoreactivity in carotid stenosis (CS). METHODS We studied the changes in the middle cerebral artery using transcranial Doppler and the breath-holding index (BHI) after hypercapnia in 33 patients with CS (15 symptomatic, 18 asymptomatic) before and 1 day and 1 month after stenting. RESULTS One day after stenting, the BHI significantly increased (p < 0.01) on the previously stenotic side in all patients. One month after stenting, the BHI was significantly higher on the contralateral side of asymptomatic (p < 0.05) and symptomatic patients (p < 0.01). CONCLUSION Percutaneous transluminal angioplasty with stenting results in increasing improvement close to normalization of impaired cerebral vasoreactivity in patients with symptomatic and asymptomatic high-grade CS.", "gemini": "BỐI CẢNH Mục đích của nghiên cứu này là đánh giá tác dụng của nong mạch qua da có đặt stent lên phản ứng co mạch não ở hẹp động mạch cảnh (CS). PHƯƠNG PHÁP Chúng tôi đã nghiên cứu những thay đổi ở động mạch não giữa bằng cách sử dụng siêu âm Doppler xuyên sọ và chỉ số nín thở (BHI) sau khi tăng CO2 ở 33 bệnh nhân bị CS (15 triệu chứng, 18 không triệu chứng) trước và 1 ngày và 1 tháng sau khi đặt stent. KẾT QUẢ Một ngày sau khi đặt stent, BHI tăng đáng kể (p < 0,01) ở bên trước đó bị hẹp ở tất cả bệnh nhân. Một tháng sau khi đặt stent, BHI cao hơn đáng kể ở bên đối diện của bệnh nhân không triệu chứng (p < 0,05) và bệnh nhân có triệu chứng (p < 0,01). KẾT LUẬN Nong mạch qua da có đặt stent giúp cải thiện đáng kể gần với mức bình thường phản ứng co mạch não bị suy yếu ở những bệnh nhân bị CS cấp độ cao có triệu chứng và không triệu chứng."} +{"idx": 1960051, "en": "BACKGROUND Many hospitalised patients with a transient ischaemic attack (TIA) or minor stroke develop subtle cognitive disorders and emotional problems a few weeks after discharge, and are dissatisfied with the care they have received, even with specialised stroke care programmes. Therefore, an individualised stroke care programme was developed to match the therapy with the personal care needs. In this pilot study we evaluated the feasibility of the rehabilitation programme, the satisfaction of stroke patients with this programme, and the factors associated with satisfaction. METHODS Consecutive patients who were hospitalised with a TIA or minor stroke, with no apparent residual deficits, were eligible. Occupational and neuropsychological screening was applied 4-6 weeks after discharge, and therapy was provided when necessary. After 6 months, satisfaction with care (Satisfaction-With-Stroke-Care-19), was assessed as the primary outcome. Secondary outcomes were quality of life (QoL), disability, handicap, anxiety and depression. RESULTS 42 patients with a TIA or minor stroke participated in the programme and the follow-up. Of all the participants, 71% were satisfied with the care they have received after discharge. Male patients were more satisfied than female patients. Dissatisfied patients were less able to perform the activities of daily living, had a lower QoL and more depressive symptoms. After regression analysis, only the Barthel Index was independently associated with satisfaction. CONCLUSIONS In this pilot study, our individualised stroke care programme seems to have improved patient satisfaction. Ability to perform daily activities is associated with satisfaction. A randomised controlled trial is needed to confirm the effectiveness of this rehabilitation programme.", "gemini": "BỐI CẢNH Nhiều bệnh nhân nhập viện bị thiếu máu não thoáng qua (TIA) hoặc đột quỵ nhẹ phát triển các rối loạn nhận thức tinh tế và các vấn đề về cảm xúc vài tuần sau khi xuất viện và không hài lòng với sự chăm sóc mà họ nhận được, ngay cả với các chương trình chăm sóc đột quỵ chuyên khoa. Do đó, một chương trình chăm sóc đột quỵ cá nhân hóa đã được phát triển để phù hợp với liệu pháp với nhu cầu chăm sóc cá nhân. Trong nghiên cứu thí điểm này, chúng tôi đã đánh giá tính khả thi của chương trình phục hồi chức năng, sự hài lòng của bệnh nhân đột quỵ với chương trình này và các yếu tố liên quan đến sự hài lòng. PHƯƠNG PHÁP Những bệnh nhân liên tiếp nhập viện vì TIA hoặc đột quỵ nhẹ, không có thiếu hụt nào còn sót lại rõ ràng, đủ điều kiện tham gia. Kiểm tra nghề nghiệp và thần kinh được áp dụng 4-6 tuần sau khi xuất viện và liệu pháp được cung cấp khi cần thiết. Sau 6 tháng, sự hài lòng với việc chăm sóc (Hài lòng-Với-Chăm sóc-Đột quỵ-19), được đánh giá là kết quả chính. Các kết quả thứ cấp là chất lượng cuộc sống (QoL), khuyết tật, tàn tật, lo lắng và trầm cảm. KẾT QUẢ 42 bệnh nhân bị TIA hoặc đột quỵ nhẹ đã tham gia chương trình và theo dõi. Trong số tất cả những người tham gia, 71% hài lòng với sự chăm sóc mà họ nhận được sau khi xuất viện. Bệnh nhân nam hài lòng hơn bệnh nhân nữ. Những bệnh nhân không hài lòng ít có khả năng thực hiện các hoạt động trong cuộc sống hàng ngày, có chất lượng cuộc sống thấp hơn và có nhiều triệu chứng trầm cảm hơn. Sau khi phân tích hồi quy, chỉ có Chỉ số Barthel có liên quan độc lập với sự hài lòng. KẾT LUẬN Trong nghiên cứu thí điểm này, chương trình chăm sóc đột quỵ cá nhân hóa của chúng tôi dường như đã cải thiện sự hài lòng của bệnh nhân. Khả năng thực hiện các hoạt động hàng ngày có liên quan đến sự hài lòng. Cần một thử nghiệm có đối chứng ngẫu nhiên để xác nhận hiệu quả của chương trình phục hồi chức năng này."} +{"idx": 1960052, "en": "BACKGROUND This study was designed to investigate the relationship between transient neurological deterioration and changes in cerebral perfusion after superficial temporal artery/middle cerebral artery (STA-MCA) bypass. METHODS Clinical characteristics of the patients with postoperative transient neurological deterioration were analyzed in 120 STA-MCA bypass procedures for ischemic cerebral diseases, such as atherosclerotic disease and moyamoya disease. Serial brain single-photon emission computed tomography (SPECT) was performed on 25 patients before surgery and on the third and tenth postoperative days. The hemispheric perfusion on SPECT was calculated semi-quantitatively using statistical parametric mapping and a probabilistic brain atlas. RESULTS Among 120 procedures, there were 4 permanent postoperative neurological deficits (3.3%) and 20 postoperative transient neurologic deteriorations with unknown etiology (17%). In 25 patients analyzed for postoperative perfusion changes, cerebral perfusion increased on the third postoperative day and the increased perfusion negatively correlated with preoperative perfusion (correlation coefficient = -0.77; p < 0.001). Temporal changes and the location of transient hyperperfusion on SPECT correlated with the time course and neurological deficits associated with postoperative transient neurological deterioration in 3 patients. CONCLUSIONS Postoperative transient relative hyperperfusion is a common phenomenon and may be one of the causes of postoperative transient neurological deterioration after STA-MCA bypass surgery for ischemic cerebral diseases.", "gemini": "BỐI CẢNH Nghiên cứu này được thiết kế để điều tra mối quan hệ giữa tình trạng thần kinh xấu đi thoáng qua và những thay đổi về tưới máu não sau khi bắc cầu động mạch thái dương nông/động mạch não giữa (STA-MCA). PHƯƠNG PHÁP Các đặc điểm lâm sàng của những bệnh nhân bị suy giảm thần kinh thoáng qua sau phẫu thuật đã được phân tích trong 120 thủ thuật bắc cầu STA-MCA đối với các bệnh não thiếu máu cục bộ, chẳng hạn như bệnh xơ vữa động mạch và bệnh moyamoya. Chụp cắt lớp phát xạ một photon não (SPECT) được thực hiện trên 25 bệnh nhân trước khi phẫu thuật và vào ngày thứ ba và thứ mười sau phẫu thuật. Tưới máu bán cầu trên SPECT được tính bán định lượng bằng cách lập bản đồ tham số thống kê và atlas não xác suất. KẾT QUẢ Trong số 120 thủ thuật, có 4 trường hợp thiếu hụt thần kinh vĩnh viễn sau phẫu thuật (3,3%) và 20 trường hợp suy giảm thần kinh thoáng qua sau phẫu thuật có nguyên nhân chưa rõ (17%). Ở 25 bệnh nhân được phân tích về những thay đổi tưới máu sau phẫu thuật, tưới máu não tăng vào ngày thứ ba sau phẫu thuật và tưới máu tăng tương quan âm với tưới máu trước phẫu thuật (hệ số tương quan = -0,77; p < 0,001). Những thay đổi về thời gian và vị trí của tình trạng tưới máu quá mức thoáng qua trên SPECT tương quan với diễn tiến thời gian và các thiếu hụt thần kinh liên quan đến tình trạng suy giảm thần kinh thoáng qua sau phẫu thuật ở 3 bệnh nhân. KẾT LUẬN Tình trạng tưới máu quá mức tương đối thoáng qua sau phẫu thuật là một hiện tượng phổ biến và có thể là một trong những nguyên nhân gây ra tình trạng suy giảm thần kinh thoáng qua sau phẫu thuật sau phẫu thuật bắc cầu STA-MCA đối với các bệnh não thiếu máu cục bộ."} +{"idx": 1960053, "en": "Metformin-associated lactic acidosis is a rare but serious condition and potentially even more hazardous during pregnancy. We reported a case of lactic acidosis in a 28-year-old pregnant woman (gravida 3, para 0, abortion 2, ante-partum 22 weeks) after ingestion of 39.50 g (approximately 80 tablets) metformin in a suicide attempt. She had no pre-existing systemic illness. Analysis of arterial blood gases revealed a high anion gap (28.1 mEq/l) and a normal osmol gap (8 mEq/l) metabolic acidosis. Other etiologies of high anion gap and normal osmol gap metabolic acidosis were excluded by laboratory investigation. The patient was treated on an emergency basis and received aggressive fluid management, intravenous sodium bicarbonate (1 mEq/kg) and activated charcoal, orally. The fetal condition was monitored intensively. The fetus was delivered smoothly via vaginal delivery in a healthy state at the 38th gestational week. Clinical follow-up over the next 2 years confirmed no congenital abnormality. We present a case of successful management of metformin-associated lactic acidosis during pregnancy, treated simply, with intravenous sodium bicarbonate and intensive fetal monitoring. This relatively noninvasive method is an effective treatment option. However, hemodialysis still has a valuable role in the management of acidosis which proves refractory to conservative treatment, such as that described.", "gemini": "Nhiễm toan lactic liên quan đến metformin là một tình trạng hiếm gặp nhưng nghiêm trọng và thậm chí có thể nguy hiểm hơn trong thời kỳ mang thai. Chúng tôi đã báo cáo một trường hợp nhiễm toan lactic ở một phụ nữ mang thai 28 tuổi (mang thai 3, sinh 0, phá thai 2, trước sinh 22 tuần) sau khi uống 39,50 g (khoảng 80 viên) metformin để tự tử. Cô ấy không có bệnh toàn thân nào trước đó. Phân tích khí máu động mạch cho thấy chênh lệch anion cao (28,1 mEq/l) và chênh lệch thẩm thấu bình thường (8 mEq/l) nhiễm toan chuyển hóa. Các nguyên nhân khác gây ra chênh lệch anion cao và chênh lệch thẩm thấu bình thường nhiễm toan chuyển hóa đã được loại trừ bằng xét nghiệm trong phòng thí nghiệm. Bệnh nhân được điều trị cấp cứu và được truyền dịch tích cực, natri bicarbonate tiêm tĩnh mạch (1 mEq/kg) và than hoạt tính dạng uống. Tình trạng của thai nhi được theo dõi chặt chẽ. Thai nhi được sinh ra suôn sẻ qua ngả âm đạo trong tình trạng khỏe mạnh ở tuần thai thứ 38. Theo dõi lâm sàng trong 2 năm tiếp theo xác nhận không có bất thường bẩm sinh. Chúng tôi trình bày một trường hợp quản lý thành công nhiễm toan lactic liên quan đến metformin trong thời kỳ mang thai, được điều trị đơn giản bằng natri bicarbonate tiêm tĩnh mạch và theo dõi chặt chẽ tình trạng của thai nhi. Phương pháp tương đối không xâm lấn này là một lựa chọn điều trị hiệu quả. Tuy nhiên, thẩm phân máu vẫn có vai trò quan trọng trong việc điều trị tình trạng nhiễm toan không đáp ứng với điều trị bảo tồn, như trường hợp đã mô tả."} +{"idx": 1960054, "en": "Although primary cilia are increasingly recognized to play sensory roles in several cellular systems, their role in vascular smooth muscle cells (VSMCs) has not been defined. We examined in situ position/orientation of primary cilia and ciliary proteins in VSMCs and tested the hypothesis that primary cilia of VSMCs exert sensory functions. By immunofluorescence and electron microscopic imaging, primary cilia of VSMCs were positioned with their long axis aligned at 58.3 degrees angle in relation to the cross-sectional plane of the artery, projecting into the extracellular matrix (ECM). Polycystin-1, polycystin-2 and alpha 3- and beta1-integrins are present in cilia. In scratch wound experiments, the majority of cilia were repositioned to the cell-wound interface. Such repositioning was largely abolished by a beta1-integrin blocker. Moreover, compared to non-ciliated/deciliated cells, ciliated VSMCs showed more efficient migration in wound repair. Lastly, when directly stimulated with collagen (an ECM component and cognate ligand for alpha 3beta1-integrins) or induced ciliary deflection, VSMCs responded with a rise in [Ca(2+)](i) that is dependent on the presence of cilia. Taken together, primary cilia of VSMCs are preferentially oriented, possess proteins critical for cell-ECM interaction and mechanosensing and respond to ECM protein and mechanical stimulations. These observations suggest a role for primary cilia in mechanochemical sensing in vasculature.", "gemini": "Mặc dù vai trò cảm giác của lông mao nguyên phát ngày càng được công nhận trong một số hệ thống tế bào, nhưng vai trò của chúng trong các tế bào cơ trơn mạch máu (VSMC) vẫn chưa được xác định. Chúng tôi đã kiểm tra vị trí/định hướng tại chỗ của lông mao nguyên phát và protein lông mao trong VSMC và kiểm tra giả thuyết rằng lông mao nguyên phát của VSMC thực hiện chức năng cảm giác. Bằng phương pháp miễn dịch huỳnh quang và chụp ảnh kính hiển vi điện tử, lông mao nguyên phát của VSMC được định vị với trục dài của chúng được căn chỉnh ở góc 58,3 độ so với mặt cắt ngang của động mạch, nhô ra vào ma trận ngoại bào (ECM). Polycystin-1, polycystin-2 và alpha 3- và beta1-integrin có trong lông mao. Trong các thí nghiệm trầy xước vết thương, phần lớn lông mao được định vị lại thành giao diện vết thương tế bào. Sự định vị lại như vậy phần lớn bị chặn bởi chất chặn beta1-integrin. Hơn nữa, so với các tế bào không có lông mao/đã rụng lông mao, các VSMC có lông mao cho thấy khả năng di chuyển hiệu quả hơn trong quá trình sửa chữa vết thương. Cuối cùng, khi được kích thích trực tiếp bằng collagen (thành phần ECM và phối tử nhận biết cho alpha 3beta1-integrin) hoặc gây lệch lông mao, VSMC phản ứng bằng cách tăng [Ca(2+)](i) phụ thuộc vào sự hiện diện của lông mao. Tổng hợp lại, lông mao nguyên phát của VSMC có định hướng ưu tiên, sở hữu các protein quan trọng đối với tương tác tế bào-ECM và cảm biến cơ học và phản ứng với protein ECM và kích thích cơ học. Những quan sát này gợi ý vai trò của lông mao nguyên phát trong cảm biến cơ học hóa học trong mạch máu."} +{"idx": 1960055, "en": "BACKGROUND/AIMS De novo coronary atherosclerosis may be involved in the poor prognosis after percutaneous coronary artery intervention (PCI) in hemodialysis patients. We aimed to clarify the factors associated with de novocoronary lesion in this population. METHODS We enrolled 106 patients on hemodialysis (72 men, 34 women; mean age, 65.4 +/- 8.9 years), who had firstly received PCI with bare-metal stents for single coronary lesions and undergone follow-up coronary angiography (CAG) 6 months thereafter. Coronary lesion with stenosis of >50% diameter that was newly recognized at follow-up CAG was defined as de novo coronary stenosis. The values of biochemical parameters were determined as the means of several measurements between PCI and follow-up CAG. RESULTS Follow-up CAG revealed de novo coronary stenosis in 40 (37.7%) of the 106 hemodialysis patients who had received PCI. Stepwise multiple logistic regression analysis showed that de novo coronary lesions were strongly associated with homeostasis model assessment insulin resistance index (HOMA-IR; 1 mM x [microU/ml]: odds ratio, 7.312; p =0.001). This significant association of HOMA-IR with de novo coronary stenosis was recognized in the diabetic and nondiabetic subgroups. CONCLUSIONS Insulin resistance may be involved in the progression of nonculprit coronary atherosclerosis after PCI in hemodialysis patients.", "gemini": "BỐI CẢNH/MỤC TIÊU Xơ vữa động mạch vành mới có thể liên quan đến tiên lượng kém sau can thiệp động mạch vành qua da (PCI) ở bệnh nhân chạy thận nhân tạo. Chúng tôi nhằm mục đích làm rõ các yếu tố liên quan đến tổn thương động mạch vành mới ở nhóm bệnh nhân này. PHƯƠNG PHÁP Chúng tôi đã tuyển dụng 106 bệnh nhân chạy thận nhân tạo (72 nam, 34 nữ; tuổi trung bình, 65,4 +/- 8,9), những người đã được can thiệp PCI lần đầu bằng stent kim loại trần cho các tổn thương động mạch vành đơn lẻ và đã được chụp động mạch vành theo dõi (CAG) 6 tháng sau đó. Tổn thương động mạch vành có hẹp đường kính >50% mới được phát hiện tại CAG theo dõi được định nghĩa là hẹp động mạch vành mới. Các giá trị của các thông số sinh hóa được xác định là giá trị trung bình của một số phép đo giữa PCI và CAG theo dõi. KẾT QUẢ CAG theo dõi cho thấy hẹp động mạch vành mới ở 40 (37,7%) trong số 106 bệnh nhân chạy thận nhân tạo đã được can thiệp PCI. Phân tích hồi quy logistic đa biến từng bước cho thấy các tổn thương động mạch vành mới có liên quan chặt chẽ với chỉ số kháng insulin của đánh giá mô hình cân bằng nội môi (HOMA-IR; 1 mM x [microU/ml]: tỷ lệ nguy cơ, 7,312; p = 0,001). Sự liên quan đáng kể này của HOMA-IR với hẹp động mạch vành mới được ghi nhận ở cả nhóm bệnh nhân tiểu đường và không tiểu đường. KẾT LUẬN Kháng insulin có thể liên quan đến sự tiến triển của xơ vữa động mạch vành không phải nguyên nhân sau can thiệp PCI ở bệnh nhân chạy thận nhân tạo."} +{"idx": 1960056, "en": "BACKGROUND To review our experience in the treatment of deep neck abscesses, including analysis of the contributing factors related to the life-threatening complications and the effects of empiric antibiotics on the outcomes. METHODS A retrospective study and statistical analysis of patients with deep neck abscesses treated at Chang Gung Memorial Hospital between April 2000 and April 2006. RESULTS A total of 105 patients were enrolled in this study, including 66 males and 39 females with age ranging from 18 to 93 years. The result of logistic regression showed that old age, patients with underlying systemic diseases (p<0.05) and ineffective empiric antibiotics (p<0.01) had statistically significant correlation with life-threatening complications. When the age was older than 65 years, the empiric antibiotics were not effective, the way of drainage of the abscess was transcervical incision, the initial deep neck abscess involved more than 1 space, or the patient had underlying systemic diseases or complications, the duration of hospital stay tended to be longer (p <0.01). CONCLUSIONS Even when adequately draining the abscess, the treatment of deep neck abscess in old-age patients more than 65 years, or the patients with ineffective empiric antibiotics or underlying systemic diseases should be more aggressive because life-threatening complications happen more frequently.", "gemini": "BỐI CẢNH Nhằm đánh giá lại kinh nghiệm của chúng tôi trong điều trị áp xe cổ sâu, bao gồm phân tích các yếu tố góp phần liên quan đến các biến chứng đe dọa tính mạng và tác động của kháng sinh kinh nghiệm đến kết quả điều trị. PHƯƠNG PHÁP Một nghiên cứu hồi cứu và phân tích thống kê về những bệnh nhân bị áp xe cổ sâu được điều trị tại Bệnh viện Tưởng niệm Trường Cung trong khoảng thời gian từ tháng 4 năm 2000 đến tháng 4 năm 2006. KẾT QUẢ Tổng cộng có 105 bệnh nhân được đưa vào nghiên cứu này, bao gồm 66 nam và 39 nữ với độ tuổi từ 18 đến 93. Kết quả hồi quy logistic cho thấy tuổi cao, bệnh nhân có bệnh toàn thân tiềm ẩn (p < 0,05) và kháng sinh kinh nghiệm không hiệu quả (p < 0,01) có mối tương quan có ý nghĩa thống kê với các biến chứng đe dọa tính mạng. Khi tuổi cao hơn 65, kháng sinh kinh nghiệm không hiệu quả, cách dẫn lưu áp xe là rạch xuyên cổ, áp xe cổ sâu ban đầu liên quan đến nhiều hơn 1 khoảng trống hoặc bệnh nhân có bệnh toàn thân hoặc biến chứng tiềm ẩn, thời gian nằm viện có xu hướng dài hơn (p < 0,01). KẾT LUẬN Ngay cả khi dẫn lưu áp xe đầy đủ, việc điều trị áp xe cổ sâu ở bệnh nhân cao tuổi trên 65 tuổi hoặc bệnh nhân dùng kháng sinh kinh nghiệm không hiệu quả hoặc có bệnh toàn thân tiềm ẩn nên tích cực hơn vì các biến chứng đe dọa tính mạng xảy ra thường xuyên hơn."} +{"idx": 1960057, "en": "Estrogen-induced proliferation in estrogen receptor (ER)-positive breast cancer cells is primarily mediated through two distinct intracellular receptors, ER alpha and ER beta. Although tumor necrosis factor alpha (TNF alpha) and E2/ER alpha are known to exert opposing effects on cell proliferation in MCF-7 cells, the mechanism by which TNFalpha antagonizes E2/ER alpha-mediated cell proliferation is not well understood. The present study suggests that reduced cell survival in response to TNF alpha treatment in MCF-7 cells may be associated with the down-regulation of ER alpha protein. The decrease in ER alpha protein level was accompanied by an inhibition of ER alpha gene transcription. Cell viability was decreased synergistically by the combined treatment with ER alpha-siRNA and TNF alpha. Furthermore, pretreatment of cells with the PI3-kinase (PI3K)/Akt inhibitor, LY294002, markedly enhanced TNF alpha-induced down-regulation of the ER alpha protein, suggesting that the PI3K/Akt pathway might be involved in control of the ER alpha level. Moreover, down-regulation of ER alpha by TNF alpha was not inhibited in cells that were pretreated with the proteasome inhibitors, MG132 and MG152, which suggests that proteasome-dependent proteolysis does not significantly influence TNF alpha-induced down-regulation of ER alpha protein. In contrast, the effect of the PI3K/Akt inhibitor on ER alpha was blocked in cells that were treated with LY294002 in the presence of the proteasome inhibitors. Collectively, our findings show that the TNF alpha may partly regulate the growth of MCF-7 breast cancer cells through the down-regulation of ER alpha expression, which is primarily mediated by a PI3K/Akt signaling.", "gemini": "Sự gia tăng do estrogen gây ra trong các tế bào ung thư vú dương tính với thụ thể estrogen (ER) chủ yếu được trung gian thông qua hai thụ thể nội bào riêng biệt, ER alpha và ER beta. Mặc dù yếu tố hoại tử khối u alpha (TNF alpha) và E2/ER alpha được biết là có tác dụng đối nghịch nhau đối với sự tăng sinh tế bào trong các tế bào MCF-7, cơ chế mà TNFalpha đối kháng với sự tăng sinh tế bào do E2/ER alpha trung gian vẫn chưa được hiểu rõ. Nghiên cứu hiện tại cho thấy rằng sự sống còn của tế bào giảm đi để đáp ứng với điều trị TNF alpha trong các tế bào MCF-7 có thể liên quan đến sự điều hòa giảm protein ER alpha. Sự giảm mức protein ER alpha đi kèm với sự ức chế phiên mã gen ER alpha. Khả năng sống của tế bào bị giảm theo hiệp đồng bởi sự kết hợp điều trị với ER alpha-siRNA và TNF alpha. Hơn nữa, tiền xử lý các tế bào bằng chất ức chế PI3-kinase (PI3K)/Akt, LY294002, làm tăng đáng kể sự điều hòa giảm của TNF alpha gây ra đối với protein ER alpha, cho thấy rằng con đường PI3K/Akt có thể liên quan đến việc kiểm soát mức ER alpha. Hơn nữa, sự điều hòa giảm ER alpha bởi TNF alpha không bị ức chế trong các tế bào được tiền xử lý bằng chất ức chế proteasome, MG132 và MG152, điều này cho thấy rằng sự phân giải protein phụ thuộc proteasome không ảnh hưởng đáng kể đến sự điều hòa giảm protein ER alpha do TNF alpha gây ra. Ngược lại, tác dụng của chất ức chế PI3K/Akt đối với ER alpha bị chặn trong các tế bào được xử lý bằng LY294002 khi có chất ức chế proteasome. Nhìn chung, những phát hiện của chúng tôi cho thấy rằng TNF alpha có thể điều chỉnh một phần sự phát triển của các tế bào ung thư vú MCF-7 thông qua sự điều hòa giảm biểu hiện ER alpha, chủ yếu được trung gian bởi tín hiệu PI3K/Akt."} +{"idx": 1960058, "en": "Microsatellites or simple sequence repeats (SSR) are widely distributed in eukaryotic genomes and are informative genetic markers. Despite many advantages of SSR markers such as a high degree of allelic polymorphisms, co-dominant inheritance, multi-allelism, and genome-wide coverage in various plant species, they also have shortcomings such as low polymorphic rates between genetically close lines, especially in Capsicum annuum. We developed an alternative technique to SSR by normalizing and alternating anchored primers in random amplified microsatellite polymorphisms (RAMP). This technique, designated reverse random amplified microsatellite polymorphism (rRAMP), allows the detection of nucleotide variation in the 3' region flanking an SSR using normalized anchored and random primer combinations. The reproducibility and frequency of polymorphic loci in rRAMP was vigorously enhanced by translocation of the 5' anchor of repeat sequences to the 3' end position and selective use of moderate arbitrary primers. In our study, the PCR banding pattern of rRAMP was highly dependent on the frequency of repeat motifs and primer combinations with random primers. Linkage analysis showed that rRAMP markers were well scattered on an intra-specific pepper map. Based on these results, we suggest that this technique is useful for studying genetic diversity, molecular fingerprinting, and rapidly constructing molecular maps for diverse plant species.", "gemini": "Các vi vệ tinh hoặc các đoạn lặp lại trình tự đơn giản (SSR) được phân bố rộng rãi trong bộ gen của sinh vật nhân chuẩn và là các dấu hiệu di truyền có giá trị thông tin. Mặc dù có nhiều ưu điểm của các dấu hiệu SSR như mức độ đa hình alen cao, di truyền đồng trội, đa alen và phạm vi phủ sóng toàn bộ bộ gen ở nhiều loài thực vật khác nhau, chúng cũng có những khuyết điểm như tỷ lệ đa hình thấp giữa các dòng gần về mặt di truyền, đặc biệt là ở Capsicum annuum. Chúng tôi đã phát triển một kỹ thuật thay thế cho SSR bằng cách chuẩn hóa và thay thế các đoạn mồi neo trong các đa hình vi vệ tinh khuếch đại ngẫu nhiên (RAMP). Kỹ thuật này, được gọi là đa hình vi vệ tinh khuếch đại ngẫu nhiên đảo ngược (rRAMP), cho phép phát hiện sự biến đổi nucleotide ở vùng 3' bao quanh SSR bằng cách sử dụng các đoạn mồi neo chuẩn hóa và các tổ hợp đoạn mồi ngẫu nhiên. Khả năng tái tạo và tần suất của các vị trí đa hình trong rRAMP được tăng cường mạnh mẽ bằng cách chuyển đoạn neo 5' của trình tự lặp lại sang vị trí đầu 3' và sử dụng chọn lọc các đoạn mồi tùy ý vừa phải. Trong nghiên cứu của chúng tôi, kiểu dải PCR của rRAMP phụ thuộc rất nhiều vào tần suất của các mô típ lặp lại và các tổ hợp đoạn mồi với các đoạn mồi ngẫu nhiên. Phân tích liên kết cho thấy các dấu hiệu rRAMP được phân tán tốt trên bản đồ ớt trong loài. Dựa trên những kết quả này, chúng tôi cho rằng kỹ thuật này hữu ích cho việc nghiên cứu sự đa dạng di truyền, dấu vân tay phân tử và nhanh chóng xây dựng các bản đồ phân tử cho các loài thực vật đa dạng."} +{"idx": 1960059, "en": "Several lines of evidence indicate that the oxidative modification of protein and the subsequent accumulation of the modified proteins have been found in cells during aging, oxidative stress, and in various pathological states including premature diseases, muscular dystrophy, rheumatoid arthritis, and atherosclerosis. The important agents that give rise to the modification of a protein may be represented by reactive aldehydic intermediates, such as ketoaldehydes, 2-alkenals and 4-hydroxy-2-alkenals. These reactive aldehydes are considered important mediators of cell damage due to their ability to covalently modify biomolecules, which can disrupt important cellular functions and can cause mutations. Furthermore, the adduction of aldehydes to apolipoprotein B in low-density lipoproteins (LDL) has been strongly implicated in the mechanism by which LDL is converted to an atherogenic form that is taken up by macrophages, leading to the formation of foam cells. During the search for an endogenous inducer of cyclooxygenase-2 (COX-2), an inducible isoform responsible for high levels of prostaglandin production during inflammation and immune responses, 4-hydroxy-2-noennal (HNE), one of the most representative lipid peroxidation product, has been identified as the potential inducer of COX-2. In addition, the following study on the molecular mechanism of the COX-2 induction by HNE has unequivocally established that a serum component, which is eventually identified to be denatured LDL, is essential for COX-2 induction. Here I review current understanding of the mechanisms by which HNE in cooperation with the serum component activates gene expression of COX-2.", "gemini": "Một số bằng chứng cho thấy rằng sự biến đổi oxy hóa của protein và sự tích tụ sau đó của các protein đã biến đổi được tìm thấy trong các tế bào trong quá trình lão hóa, stress oxy hóa và trong các trạng thái bệnh lý khác nhau bao gồm các bệnh sớm, loạn dưỡng cơ, viêm khớp dạng thấp và xơ vữa động mạch. Các tác nhân quan trọng gây ra sự biến đổi của protein có thể được biểu thị bằng các chất trung gian aldehyt phản ứng, chẳng hạn như ketoaldehyd, 2-alkenal và 4-hydroxy-2-alkenal. Các aldehyt phản ứng này được coi là chất trung gian quan trọng gây tổn thương tế bào do khả năng biến đổi sinh học các phân tử sinh học, có thể phá vỡ các chức năng quan trọng của tế bào và có thể gây ra đột biến. Hơn nữa, sự kết hợp của các aldehyt với apolipoprotein B trong lipoprotein tỷ trọng thấp (LDL) có liên quan chặt chẽ đến cơ chế mà LDL được chuyển đổi thành dạng gây xơ vữa động mạch được đại thực bào hấp thụ, dẫn đến sự hình thành các tế bào bọt. Trong quá trình tìm kiếm chất gây cảm ứng nội sinh của cyclooxygenase-2 (COX-2), một dạng đồng phân cảm ứng chịu trách nhiệm cho mức sản xuất prostaglandin cao trong quá trình viêm và phản ứng miễn dịch, 4-hydroxy-2-noennal (HNE), một trong những sản phẩm peroxy hóa lipid tiêu biểu nhất, đã được xác định là chất gây cảm ứng COX-2 tiềm năng. Ngoài ra, nghiên cứu sau đây về cơ chế phân tử của sự cảm ứng COX-2 bởi HNE đã xác lập rõ ràng rằng một thành phần huyết thanh, cuối cùng được xác định là LDL đã biến tính, là rất cần thiết cho sự cảm ứng COX-2. Ở đây, tôi xem xét sự hiểu biết hiện tại về các cơ chế mà HNE hợp tác với thành phần huyết thanh kích hoạt biểu hiện gen của COX-2."} +{"idx": 1960060, "en": "Plants are continually exposed to a variety of potentially pathogenic microbes, and the interactions between plants and pathogenic invaders determine the outcome, disease or disease resistance. To defend themselves, plants have developed a sophisticated immune system. Unlike animals, however, they do not have specialized immune cells and, thus all plant cells appear to have the innate ability to recognize pathogens and turn on an appropriate defense response. Using genetic, genomic and biochemical methods, tremendous advances have been made in understanding how plants recognize pathogens and mount effective defenses. The primary immune response is induced by microbe-associated molecular patterns (MAMPs). MAMP receptors recognize the presence of probable pathogens and evoke defense. In the co-evolution of plant-microbe interactions, pathogens gained the ability to make and deliver effector proteins to suppress MAMP-induced defense responses. In response to effector proteins, plants acquired R-proteins to directly or indirectly monitor the presence of effector proteins and activate an effective defense response. In this review we will describe and discuss the plant immune responses induced by two types of elicitors, PAMPs and effector proteins.", "gemini": "Cây liên tục tiếp xúc với nhiều loại vi khuẩn có khả năng gây bệnh và sự tương tác giữa cây và những kẻ xâm lược gây bệnh quyết định kết quả là bệnh hay sức đề kháng với bệnh. Để tự vệ, thực vật đã phát triển một hệ thống miễn dịch tinh vi. Tuy nhiên, không giống như động vật, chúng không có các tế bào miễn dịch chuyên biệt và do đó, tất cả các tế bào thực vật dường như đều có khả năng bẩm sinh là nhận ra các tác nhân gây bệnh và kích hoạt phản ứng phòng vệ thích hợp. Sử dụng các phương pháp di truyền, gen và sinh hóa, những tiến bộ to lớn đã đạt được trong việc hiểu cách thực vật nhận ra các tác nhân gây bệnh và tạo ra hàng phòng thủ hiệu quả. Phản ứng miễn dịch chính được gây ra bởi các kiểu mẫu phân tử liên kết với vi khuẩn (MAMP). Các thụ thể MAMP nhận ra sự hiện diện của các tác nhân gây bệnh có thể có và kích hoạt phản ứng phòng vệ. Trong quá trình tiến hóa chung của tương tác thực vật-vi khuẩn, các tác nhân gây bệnh đã có khả năng tạo ra và cung cấp các protein tác động để ức chế các phản ứng phòng vệ do MAMP gây ra. Để đáp ứng với các protein tác động, thực vật đã có được các protein R để theo dõi trực tiếp hoặc gián tiếp sự hiện diện của các protein tác động và kích hoạt phản ứng phòng vệ hiệu quả. Trong bài đánh giá này, chúng tôi sẽ mô tả và thảo luận về các phản ứng miễn dịch của thực vật được gây ra bởi hai loại tác nhân gây bệnh, PAMP và protein tác động."} +{"idx": 1960061, "en": "S-glutathionylation is a reversible post-translational modification that continues to gain eminence as a redox regulatory mechanism of protein activity and associated cellular functions. Many diverse cellular proteins such as transcription factors, adhesion molecules, enzymes, and cytokines are reported to undergo glutathionylation, although the functional impact has been less well characterized. De-glutathionylation is catalyzed specifically and efficiently by glutaredoxin (GRx, aka thioltransferase), and facile reversibility is critical in determining the physiological relevance of glutathionylation as a means of protein regulation. Thus, studies with cohesive themes addressing both the glutathionylation of proteins and the corresponding impact of GRx are especially useful in advancing understanding. Reactive oxygen species (ROS) and redox regulation are well accepted as playing a role in inflammatory processes, such as leukostasis and the destruction of foreign particles by macrophages. We discuss in this review the current implications of GRx and/or glutathionylation in the inflammatory response and in diseases associated with chronic inflammation, namely diabetes, atherosclerosis, inflammatory lung disease, cancer, and Alzheimer's disease, and in viral infections.", "gemini": "S-glutathionylation là một sửa đổi sau dịch mã có thể đảo ngược, tiếp tục nổi bật như một cơ chế điều chỉnh hoạt động của protein và các chức năng tế bào liên quan thông qua quá trình oxy hóa khử. Nhiều loại protein tế bào khác nhau như yếu tố phiên mã, phân tử bám dính, enzyme và cytokine được báo cáo là trải qua quá trình glutathionylation, mặc dù tác động về mặt chức năng vẫn chưa được mô tả rõ ràng. Quá trình khử glutathionylation được xúc tác một cách đặc hiệu và hiệu quả bởi glutaredoxin (GRx, còn gọi là thioltransferase), và khả năng đảo ngược dễ dàng rất quan trọng trong việc xác định tính liên quan về mặt sinh lý của quá trình glutathionylation như một phương tiện điều hòa protein. Do đó, các nghiên cứu với các chủ đề gắn kết giải quyết cả quá trình glutathionylation của protein và tác động tương ứng của GRx đặc biệt hữu ích trong việc thúc đẩy sự hiểu biết. Các loài oxy phản ứng (ROS) và quá trình điều hòa oxy hóa khử được chấp nhận rộng rãi là đóng vai trò trong các quá trình viêm, chẳng hạn như tình trạng λευκώ và sự phá hủy các hạt lạ bởi các đại thực bào. Trong bài đánh giá này, chúng tôi thảo luận về những tác động hiện tại của GRx và/hoặc quá trình glutathionylation trong phản ứng viêm và trong các bệnh liên quan đến tình trạng viêm mãn tính, cụ thể là bệnh tiểu đường, xơ vữa động mạch, bệnh phổi viêm, ung thư và bệnh Alzheimer, cũng như trong các bệnh nhiễm virus."} +{"idx": 1960062, "en": "Burkholderia pseudomallei is the causative agent of melioidosis, a tropical infection of humans and other animals. The bacterium is an intracellular pathogen that can escape from endosomes into the host cytoplasm, where it replicates and infects adjacent cells. We investigated the role played by autophagy in the intracellular survival of B. pseudomallei in phagocytic and non-phagocytic cell lines. Autophagy was induced in response to B. pseudomallei invasion of murine macrophage (RAW 264.7) cells and a proportion of the bacteria co-localized with the autophagy effector protein LC3, a marker for autophagosome formation. Pharmacological stimulation of autophagy in RAW 264.7 and murine embryonic fibroblast (MEF) cell lines resulted in increased co-localization of B. pseudomallei with LC3 while basal levels of co-localization could be abrogated using inhibitors of the autophagic pathway. Furthermore, induction of autophagy decreased the intracellular survival of B. pseudomallei in these cell lines, but bacterial survival was not affected in MEF cell lines deficient in autophagy. Treatment of infected macrophages with chloramphenicol increased the proportion of bacteria within autophagosomes indicating that autophagic evasion is an active process relying on bacterial protein synthesis. Consistent with this hypothesis, we identified a B. pseudomallei type III secreted protein, BopA, which plays a role in mediating bacterial evasion of autophagy. We conclude that the autophagic pathway is a component of the innate defense system against invading B. pseudomallei, but which the bacteria can actively evade. However, when autophagy is pharmacologically induced using rapamycin, bacteria are actively sequestered in autophagosomes, ultimately decreasing their survival.", "gemini": "Burkholderia pseudomallei là tác nhân gây bệnh melioidosis, một bệnh nhiễm trùng nhiệt đới ở người và động vật khác. Vi khuẩn này là một tác nhân gây bệnh nội bào có thể thoát khỏi các thể nội bào vào tế bào chất của vật chủ, nơi nó sao chép và lây nhiễm vào các tế bào liền kề. Chúng tôi đã nghiên cứu vai trò của autophagy trong sự sống sót của B. pseudomallei bên trong tế bào trong các dòng tế bào thực bào và không thực bào. Autophagy được gây ra để đáp ứng với sự xâm nhập của B. pseudomallei vào các tế bào đại thực chuột (RAW 264.7) và một tỷ lệ vi khuẩn được định vị cùng với protein tác động autophagy LC3, một dấu hiệu cho sự hình thành thể tự thực. Kích thích dược lý autophagy trong các dòng tế bào RAW 264.7 và nguyên bào sợi phôi chuột (MEF) dẫn đến sự định vị cùng nhau tăng lên của B. pseudomallei với LC3 trong khi mức cơ bản của sự định vị cùng nhau có thể bị loại bỏ bằng cách sử dụng các chất ức chế con đường tự thực. Hơn nữa, việc gây ra autophagy làm giảm sự sống sót của B. pseudomallei bên trong tế bào trong các dòng tế bào này, nhưng sự sống sót của vi khuẩn không bị ảnh hưởng trong các dòng tế bào MEF bị thiếu autophagy. Việc điều trị các đại thực bào bị nhiễm bằng chloramphenicol làm tăng tỷ lệ vi khuẩn trong các thể tự thực, cho thấy rằng quá trình trốn tránh tự thực là một quá trình chủ động dựa vào quá trình tổng hợp protein của vi khuẩn. Phù hợp với giả thuyết này, chúng tôi đã xác định được một loại protein tiết loại III của B. pseudomallei, BopA, đóng vai trò trung gian trong quá trình vi khuẩn trốn tránh tự thực. Chúng tôi kết luận rằng con đường tự thực là một thành phần của hệ thống phòng vệ bẩm sinh chống lại sự xâm nhập của B. pseudomallei, nhưng vi khuẩn có thể chủ động trốn tránh. Tuy nhiên, khi autophagy được gây ra bằng dược lý sử dụng rapamycin, vi khuẩn sẽ bị cô lập chủ động trong các thể tự thực, cuối cùng làm giảm sự sống sót của chúng."} +{"idx": 1960063, "en": "BACKGROUND This study was designed to investigate the characteristics, control rate, and factors affecting BP control in hypertensive patients treated by primary physicians in Korea. METHODS The study was a multicenter, nationwide, cross-sectional, population-based survey conducted to identify the current status of hypertension treatment in Korean patients. A direct mail survey was sent to a random sample of 800 primary-care clinics across the nation. A total of 529 physicians in 510 clinics agreed to participate in this study (63.8%). Among the recruited 13,452 patients, 13,184 were included in the analysis. RESULTS BP was controlled in 6,723 patients (51.0%). However, the control rate was lower in patients with diabetes (21.6%) or chronic kidney disease (CKD) (19.9%). Furthermore, the mean systolic and diastolic BP levels were higher in patients with diabetes and/or CKD. The mean BP level was higher in patients undergoing combination therapy than those undergoing single-drug therapy. Male gender, cardiovascular comorbidities, long duration of hypertension, and unhealthy lifestyle were associated with poor control of hypertension. In multivariate analysis, diabetes (odds ratio: 5.57; 95% confidence interval: 5.05-6.13) and CKD (odds ratio: 3.40; 95% confidence interval: 2.54-4.54) were the most significant independent factors related to poor BP control. CONCLUSION BP control is still largely unsatisfactory, especially in patients with diabetes and kidney disease, who could benefit the most from effective BP control. The reasons for poor BP control and high BP levels in those patients need to be investigated to improve BP control in Korea.", "gemini": "BỐI CẢNH Nghiên cứu này được thiết kế để điều tra các đặc điểm, tỷ lệ kiểm soát và các yếu tố ảnh hưởng đến việc kiểm soát huyết áp ở bệnh nhân tăng huyết áp được điều trị bởi các bác sĩ đa khoa tại Hàn Quốc. PHƯƠNG PHÁP Nghiên cứu là một cuộc khảo sát cắt ngang, dựa trên dân số, trên toàn quốc, đa trung tâm được tiến hành để xác định tình trạng hiện tại của việc điều trị tăng huyết áp ở bệnh nhân Hàn Quốc. Một cuộc khảo sát qua thư trực tiếp đã được gửi đến một mẫu ngẫu nhiên gồm 800 phòng khám chăm sóc ban đầu trên toàn quốc. Tổng cộng có 529 bác sĩ ở 510 phòng khám đồng ý tham gia nghiên cứu này (63,8%). Trong số 13.452 bệnh nhân được tuyển dụng, có 13.184 bệnh nhân được đưa vào phân tích. KẾT QUẢ Huyết áp được kiểm soát ở 6.723 bệnh nhân (51,0%). Tuy nhiên, tỷ lệ kiểm soát thấp hơn ở những bệnh nhân bị tiểu đường (21,6%) hoặc bệnh thận mãn tính (CKD) (19,9%). Hơn nữa, mức huyết áp tâm thu và huyết áp tâm trương trung bình cao hơn ở những bệnh nhân bị tiểu đường và/hoặc CKD. Mức huyết áp trung bình cao hơn ở những bệnh nhân đang điều trị kết hợp so với những bệnh nhân đang điều trị bằng một loại thuốc. Giới tính nam, bệnh tim mạch đi kèm, thời gian bị tăng huyết áp kéo dài và lối sống không lành mạnh có liên quan đến việc kiểm soát huyết áp kém. Trong phân tích đa biến, tiểu đường (tỷ lệ cược: 5,57; khoảng tin cậy 95%: 5,05-6,13) và CKD (tỷ lệ cược: 3,40; khoảng tin cậy 95%: 2,54-4,54) là những yếu tố độc lập quan trọng nhất liên quan đến việc kiểm soát huyết áp kém. KẾT LUẬN Việc kiểm soát huyết áp vẫn chưa đạt yêu cầu, đặc biệt là ở những bệnh nhân bị tiểu đường và bệnh thận, những người có thể hưởng lợi nhiều nhất từ việc kiểm soát huyết áp hiệu quả. Cần phải điều tra lý do kiểm soát huyết áp kém và mức huyết áp cao ở những bệnh nhân này để cải thiện việc kiểm soát huyết áp ở Hàn Quốc."} +{"idx": 1960064, "en": "BACKGROUND Elevated night time/daytime blood pressure (BP) ratios are associated with cardiovascular morbidity and mortality. We evaluated the associations between sleep/awake BP ratios and sleep disturbances. METHODS Sleep disturbances were assessed by in-home actigraphy and diary measures for nine nights, and polysomnography (PSG) for two nights; ambulatory BP was measured for at least 48 h. Participants were 186 middle-aged African-American and Caucasian men and women who were free from prevalent myocardial infarction, stroke, history of interventional cardiology procedures, diabetes, and diagnosed apnea or other sleep disorders. RESULTS Results showed that the greater the sleep/wake ratios of BP, the more fragmented the sleep, the greater the proportion in stage 1 (light) sleep and the smaller the proportion in rapid eye movement (REM) sleep, and the greater the number of arousals from sleep. These results were independent of age, race, gender, Framingham Risk status, cardiovascular medications, body mass index, and apnea/hypopnea index. Indicators of psychosocial stress were not greater among those with higher sleep/wake BP ratios. CONCLUSIONS Findings are consistent with the hypothesis that elevated night time/daytime pressure may be a consequence of poor sleep.", "gemini": "BỐI CẢNH Tỷ lệ huyết áp (BP) ban đêm/ban ngày cao hơn có liên quan đến bệnh tim mạch và tử vong. Chúng tôi đã đánh giá mối liên quan giữa tỷ lệ huyết áp khi ngủ/thức và các rối loạn giấc ngủ. PHƯƠNG PHÁP Các rối loạn giấc ngủ được đánh giá bằng phương pháp đo hoạt động trong nhà và nhật ký trong chín đêm, và bằng phương pháp chụp điện não đồ (PSG) trong hai đêm; huyết áp lưu động được đo trong ít nhất 48 giờ. Những người tham gia là 186 nam và nữ trung niên người Mỹ gốc Phi và người da trắng không mắc nhồi máu cơ tim, đột quỵ, tiền sử can thiệp tim mạch, tiểu đường và được chẩn đoán là ngưng thở khi ngủ hoặc các rối loạn giấc ngủ khác. KẾT QUẢ Kết quả cho thấy tỷ lệ huyết áp khi ngủ/thức càng lớn thì giấc ngủ càng rời rạc, tỷ lệ ở giai đoạn 1 (ngủ nhẹ) càng lớn và tỷ lệ ở giai đoạn chuyển động mắt nhanh (REM) càng nhỏ và số lần thức giấc càng nhiều. Những kết quả này không phụ thuộc vào tuổi tác, chủng tộc, giới tính, tình trạng nguy cơ Framingham, thuốc tim mạch, chỉ số khối cơ thể và chỉ số ngưng thở/giảm thông khí. Các chỉ số căng thẳng tâm lý xã hội không cao hơn ở những người có tỷ lệ huyết áp khi ngủ/thức cao hơn. KẾT LUẬN Các phát hiện phù hợp với giả thuyết rằng áp lực ban đêm/ban ngày cao hơn có thể là hậu quả của giấc ngủ kém."} +{"idx": 1960065, "en": "The export of quality-controlled mRNAs across the nuclear membrane for translation is mediated by a set of core proteins that are conserved throughout eukaryotes and have been best-characterized in Saccharomyces cerevisiae. The increased genomic complexity that arose during metazoan evolution, however, has resulted in increased systematic complexity that is reflected in the presence of additional metazoan mRNA export factors. In some cases, metazoans encode families of closely-related factors whereas in fungi, a single homolog is present. An exciting new study examines metazoan mRNA export from a global perspective through the use of a genome-wide RNA interference screen in Drosophila melanogaster. This screen identified several novel factors that contribute to mRNA export while reaffirming the strong evolutionary conservation that exists between Drosophila and yeast homologs that are essential for mRNA export. The study also showed that several factors were specifically required for the export of an intron-containing transcript but not for one lacking an intron. Taken together, this study underscores the value of genomic approaches for understanding complex biological processes.", "gemini": "Việc xuất các mRNA được kiểm soát chất lượng qua màng nhân để dịch mã được trung gian bởi một tập hợp các protein cốt lõi được bảo tồn trong suốt quá trình tiến hóa của sinh vật nhân chuẩn và đã được đặc trưng tốt nhất ở Saccharomyces cerevisiae. Tuy nhiên, sự phức tạp về bộ gen gia tăng phát sinh trong quá trình tiến hóa của động vật đa bào đã dẫn đến sự phức tạp có hệ thống gia tăng được phản ánh trong sự hiện diện của các yếu tố xuất mRNA động vật đa bào bổ sung. Trong một số trường hợp, động vật đa bào mã hóa các họ các yếu tố có liên quan chặt chẽ trong khi ở nấm, chỉ có một đồng đẳng duy nhất. Một nghiên cứu mới thú vị đã xem xét quá trình xuất mRNA của động vật đa bào từ góc độ toàn cầu thông qua việc sử dụng sàng lọc can thiệp RNA trên toàn bộ hệ gen ở Drosophila melanogaster. Màn hình này đã xác định một số yếu tố mới góp phần vào quá trình xuất mRNA trong khi khẳng định lại sự bảo tồn tiến hóa mạnh mẽ giữa các đồng đẳng Drosophila và nấm men rất cần thiết cho quá trình xuất mRNA. Nghiên cứu cũng chỉ ra rằng một số yếu tố được yêu cầu cụ thể để xuất một bản sao chứa intron nhưng không phải đối với một bản sao không có intron. Tổng hợp lại, nghiên cứu này nhấn mạnh giá trị của các phương pháp tiếp cận bộ gen để hiểu các quá trình sinh học phức tạp."} +{"idx": 1960066, "en": "BACKGROUND Failure to maintain weight losses in lifestyle change programs continues to be a major problem and warrants investigation of innovative approaches to weight control. OBJECTIVE The goal of this study was to compare two novel group interventions, both aimed at improving weight loss maintenance, with a control group. METHODS AND PROCEDURES A total of 103 women lost weight on a meal replacement-supplemented diet and were then randomized to one of three conditions for the 14-week maintenance phase: cognitive-behavioral treatment (CBT); CBT with an enhanced food monitoring accuracy (EFMA) program; or these two interventions plus a reduced energy density eating (REDE) program. Assessments were conducted periodically through an 18-month postintervention. Outcome measures included weight and self-reported dietary intake. Data were analyzed using completers only as well as baseline-carried-forward imputation. RESULTS Participants lost an average of 7.6 +/- 2.6 kg during the weight loss phase and 1.8 +/- 2.3 kg during the maintenance phase. Results do not suggest that the EFMA intervention was successful in improving food monitoring accuracy. The REDE group decreased the energy density (ED) of their diets more so than the other two groups. However, neither the REDE nor the EFMA condition showed any advantage in weight loss maintenance. All groups regained weight between 6- and 18-month follow-ups. DISCUSSION Although no incremental weight maintenance benefit was observed in the EFMA or EFMA + REDE groups, the improvement in the ED of the REDE group's diet, if shown to be sustainable in future studies, could have weight maintenance benefits.", "gemini": "HOÀN CẢNH Việc không duy trì được cân nặng sau khi giảm cân trong các chương trình thay đổi lối sống vẫn tiếp tục là một vấn đề lớn và cần phải điều tra các phương pháp tiếp cận sáng tạo để kiểm soát cân nặng. MỤC TIÊU Mục tiêu của nghiên cứu này là so sánh hai biện pháp can thiệp nhóm mới, đều nhằm cải thiện việc duy trì giảm cân, với một nhóm đối chứng. PHƯƠNG PHÁP VÀ THỦ TỤC Tổng cộng 103 phụ nữ đã giảm cân bằng chế độ ăn bổ sung thay thế bữa ăn và sau đó được phân ngẫu nhiên vào một trong ba điều kiện cho giai đoạn duy trì 14 tuần: điều trị nhận thức hành vi (CBT); CBT với chương trình tăng cường độ chính xác theo dõi thực phẩm (EFMA); hoặc hai biện pháp can thiệp này cộng với chương trình ăn giảm mật độ năng lượng (REDE). Đánh giá được tiến hành định kỳ trong vòng 18 tháng sau khi can thiệp. Các biện pháp kết quả bao gồm cân nặng và lượng thức ăn tự báo cáo. Dữ liệu được phân tích chỉ bằng cách hoàn thành cũng như bằng cách ước tính chuyển tiếp cơ sở. KẾT QUẢ Những người tham gia đã giảm trung bình 7,6 +/- 2,6 kg trong giai đoạn giảm cân và 1,8 +/- 2,3 kg trong giai đoạn duy trì. Kết quả không cho thấy rằng can thiệp EFMA thành công trong việc cải thiện độ chính xác theo dõi thực phẩm. Nhóm REDE giảm mật độ năng lượng (ED) trong chế độ ăn của họ nhiều hơn hai nhóm còn lại. Tuy nhiên, cả điều kiện REDE và EFMA đều không cho thấy bất kỳ lợi thế nào trong việc duy trì giảm cân. Tất cả các nhóm đều tăng cân trở lại trong quá trình theo dõi từ 6 đến 18 tháng. THẢO LUẬN Mặc dù không quan sát thấy lợi ích duy trì cân nặng gia tăng nào ở nhóm EFMA hoặc EFMA + REDE, nhưng việc cải thiện ED trong chế độ ăn của nhóm REDE, nếu được chứng minh là bền vững trong các nghiên cứu trong tương lai, có thể mang lại lợi ích duy trì cân nặng."} +{"idx": 1960067, "en": "OBJECTIVE The suckling period is one potentially \"critical\" period during which nutritional intake may permanently \"program\" metabolism to promote increased adult body weight and insulin resistance in later life. This study determined whether fructose introduced during the suckling period altered body weight and induced changes in fatty acid transport leading to insulin resistance in adulthood in rats. METHODS AND PROCEDURES Pups were randomly assigned to one of four diets: suckle controls (SCs), rat milk substitute formula (Rat Milk Substitute), fructose-containing formula (Fructose), or galactose-containing formula (Galactose). Starting at weaning, all pups received the same diet; at 8 weeks of age, half of the SC rats began ingesting a diet containing 65% kcal fructose (SC-Fructose). This continued until animals were 12 weeks old and the study ended. RESULTS At weeks 8, 10, and 11, the Fructose group weighed more than SC and SC-Fructose groups (P < 0.05). At weeks 8 and 10 of age, the Fructose group had significantly higher insulin concentrations vs. rats in the SC-Fructose group. (3)H-Palmitate transport into vesicles from hind limb skeletal muscle was higher in Fructose vs. SC rats (P < 0.05). CD36 expression was increased in the sarcolemma but not in whole tissue homogenates from skeletal muscle from Fructose rats (P < 0.05) suggesting a redistribution of this protein associated with fatty acid uptake across the plasma membrane. This change in subcellular localization of CD36 is associated with insulin resistance in muscle. DISCUSSION Consuming fructose during suckling may result in lifelong changes in body weight, insulin secretion, and fatty acid transport involving CD36 in muscle and ultimately promote insulin resistance.", "gemini": "MỤC TIÊU Giai đoạn bú là một giai đoạn \"quan trọng\" mà trong đó lượng dinh dưỡng nạp vào có thể \"lập trình\" vĩnh viễn quá trình trao đổi chất để thúc đẩy tăng cân ở người lớn và kháng insulin ở giai đoạn sau của cuộc đời. Nghiên cứu này xác định xem fructose được đưa vào trong giai đoạn bú có làm thay đổi trọng lượng cơ thể và gây ra những thay đổi trong quá trình vận chuyển axit béo dẫn đến kháng insulin ở chuột trưởng thành hay không. PHƯƠNG PHÁP VÀ THỦ TỤC Các chú chuột con được phân ngẫu nhiên vào một trong bốn chế độ ăn: đối chứng bú (SC), công thức thay thế sữa chuột (Rat Milk Substitute), công thức có chứa fructose (Fructose) hoặc công thức có chứa galactose (Galactose). Bắt đầu từ khi cai sữa, tất cả các chú chuột con đều được cho ăn cùng một chế độ ăn; ở tuần thứ 8, một nửa số chuột SC bắt đầu ăn chế độ ăn có chứa 65% kcal fructose (SC-Fructose). Điều này tiếp tục cho đến khi những con vật được 12 tuần tuổi và nghiên cứu kết thúc. KẾT QUẢ Vào tuần thứ 8, 10 và 11, nhóm Fructose nặng hơn nhóm SC và SC-Fructose (P < 0,05). Vào tuần thứ 8 và 10 tuổi, nhóm Fructose có nồng độ insulin cao hơn đáng kể so với chuột trong nhóm SC-Fructose. (3) H-Palmitate vận chuyển vào túi từ cơ xương chi sau cao hơn ở chuột Fructose so với chuột SC (P < 0,05). Biểu hiện CD36 tăng lên ở màng sarcolemma nhưng không tăng ở toàn bộ mô đồng nhất từ cơ xương của chuột Fructose (P < 0,05), cho thấy sự tái phân phối của protein này liên quan đến sự hấp thụ axit béo qua màng huyết tương. Sự thay đổi này trong vị trí dưới tế bào của CD36 có liên quan đến tình trạng kháng insulin ở cơ. THẢO LUẬN Tiêu thụ fructose trong thời kỳ bú có thể dẫn đến những thay đổi suốt đời về trọng lượng cơ thể, tiết insulin và vận chuyển axit béo liên quan đến CD36 trong cơ và cuối cùng thúc đẩy tình trạng kháng insulin."} +{"idx": 1960068, "en": "The aim of this study was to test the hypothesis of an association between the -30G>A polymorphism of the promoter of the glucokinase gene and the prevalence and incidence of obesity. We studied the -30G>A polymorphism of the glucokinase gene promoter in 981 persons, of whom 866 were seen again 6 years later. All the persons underwent an oral glucose-tolerance test and the BMI (weight/height(2)) was recorded. The -30G>A polymorphism of the glucokinase gene promoter was studied using RFLP-PCR. At the initial study, the probability of having a BMI > or =25 in carriers of the A allele was significantly lower than expected by chance (odds ratio (OR) = 0.63; 95% confidence interval (CI) = 0.456-0.885). In those persons with a BMI > or =30 at the first study, the probability at 6 years of losing weight (reaching a BMI < 30) was greater in carriers of the A allele (OR = 0.22; 95% CI = 0.087-0.576). The increase in weight over these 6 years, taken as a continuous variable, was significantly less only in those persons who were originally obese (P = 0.018). In conclusion, in a population from southern Spain, carriers of the A allele of the -30G>A polymorphism in the promoter of the glucokinase gene had a lower risk for obesity and the likelihood of losing weight was greater in those obese persons who had the A allele (GA or AA).", "gemini": "Mục đích của nghiên cứu này là kiểm tra giả thuyết về mối liên quan giữa đa hình -30G>A của vùng khởi động của gen glucokinase và tỷ lệ mắc và tỷ lệ béo phì. Chúng tôi đã nghiên cứu đa hình -30G>A của vùng khởi động gen glucokinase ở 981 người, trong đó 866 người được gặp lại sau 6 năm. Tất cả những người này đều được thực hiện xét nghiệm dung nạp glucose đường uống và ghi lại chỉ số BMI (cân nặng/chiều cao(2)). Đa hình -30G>A của vùng khởi động gen glucokinase được nghiên cứu bằng phương pháp RFLP-PCR. Ở nghiên cứu ban đầu, xác suất có BMI> hoặc = 25 ở những người mang alen A thấp hơn đáng kể so với dự kiến (tỷ lệ chênh lệch (OR) = 0,63; khoảng tin cậy 95% (CI) = 0,456-0,885). Ở những người có BMI> hoặc = 30 ở nghiên cứu đầu tiên, xác suất giảm cân sau 6 năm (đạt BMI < 30) cao hơn ở những người mang alen A (OR = 0,22; 95% CI = 0,087-0,576). Tăng cân trong 6 năm này, được coi là một biến liên tục, chỉ thấp hơn đáng kể ở những người ban đầu bị béo phì (P = 0,018). Tóm lại, trong quần thể ở miền nam Tây Ban Nha, những người mang alen A của đa hình -30G>A ở vùng khởi động của gen glucokinase có nguy cơ béo phì thấp hơn và khả năng giảm cân cao hơn ở những người béo phì có alen A (GA hoặc AA)."} +{"idx": 1960069, "en": "To examine weight-related differences in eating behaviors and nutrition of preschool-aged children, the influence of maternal eating behavior on the child's eating behavior, and sex-related differences in the transmission of eating behaviors. A total of 142 mothers of children aged 3-6 years participated. Maternal and child's eating behaviors as well as child's food consumption were assessed using questionnaires completed by mothers. Maternal BMI and child's standardized BMI (BMI-SDS) were also calculated. More than half of the mothers were obese. Multiple regression analysis was used to predict eating behavior of the children by mothers' variables. Overweight children scored higher in external eating, food responsiveness, and speed of eating than normal-weight children, whereas children of overweight mothers showed higher amounts of emotional eating than children of normal-weight mothers. Maternal emotional eating (R(2) = 0.19, P < 0.001) and mother's BMI (R(2) = 0.07, P < 0.05) positively predicted emotional eating of sons. Maternal emotional eating (R(2) = 0.19, P < 0.01) completely mediated the relation between mother's BMI and emotional eating of sons. For mother-daughter dyads, no such relation was found. The tested model shows sex-related differences in the transmission of maternal eating behavior which is discussed as being related to the development and maintenance of obesity.", "gemini": "Để kiểm tra sự khác biệt liên quan đến cân nặng trong hành vi ăn uống và dinh dưỡng của trẻ em trong độ tuổi mẫu giáo, ảnh hưởng của hành vi ăn uống của mẹ đối với hành vi ăn uống của trẻ và sự khác biệt liên quan đến giới tính trong quá trình truyền tải hành vi ăn uống. Tổng cộng có 142 bà mẹ của trẻ em từ 3-6 tuổi tham gia. Hành vi ăn uống của mẹ và trẻ cũng như lượng thức ăn tiêu thụ của trẻ được đánh giá bằng các bảng câu hỏi do các bà mẹ hoàn thành. Chỉ số BMI của mẹ và BMI chuẩn hóa (BMI-SDS) của trẻ cũng được tính toán. Hơn một nửa số bà mẹ bị béo phì. Phân tích hồi quy bội đã được sử dụng để dự đoán hành vi ăn uống của trẻ em theo các biến số của mẹ. Trẻ em thừa cân có điểm cao hơn về ăn uống bên ngoài, khả năng phản ứng với thức ăn và tốc độ ăn so với trẻ em có cân nặng bình thường, trong khi trẻ em của các bà mẹ thừa cân biểu hiện ăn uống theo cảm xúc nhiều hơn so với trẻ em của các bà mẹ có cân nặng bình thường. Ăn uống theo cảm xúc của mẹ (R(2) = 0,19, P < 0,001) và BMI của mẹ (R(2) = 0,07, P < 0,05) dự đoán tích cực việc ăn uống theo cảm xúc của con trai. Ăn uống theo cảm xúc của mẹ (R(2) = 0,19, P < 0,01) hoàn toàn có mối quan hệ trung gian giữa BMI của mẹ và việc ăn uống theo cảm xúc của con trai. Đối với các cặp mẹ-con gái, không tìm thấy mối quan hệ như vậy. Mô hình được kiểm tra cho thấy sự khác biệt liên quan đến giới tính trong quá trình truyền tải hành vi ăn uống của mẹ, được thảo luận là có liên quan đến sự phát triển và duy trì tình trạng béo phì."} +{"idx": 1960070, "en": "There is a pressing need for robust imaging markers to assist in the development of drugs for the treatment of atherosclerosis. Conventional imaging methods provide quantitative and morphological data but may be inadequate for assessing a new generation of therapies that modify plaque biology directly. Here, we compare the main imaging modalities used to image atherosclerosis in the clinical-trial setting, and assess their ability to predict clinical outcomes for a given sample size. We consider how emerging molecular and cellular imaging techniques could offer the possibility to quantify changes in biological function at the level of the plaque, even without gross structural change.", "gemini": "Có nhu cầu cấp thiết về các dấu hiệu hình ảnh mạnh mẽ để hỗ trợ phát triển thuốc điều trị xơ vữa động mạch. Các phương pháp hình ảnh thông thường cung cấp dữ liệu định lượng và hình thái nhưng có thể không đủ để đánh giá một thế hệ liệu pháp mới có khả năng sửa đổi trực tiếp sinh học của mảng bám. Tại đây, chúng tôi so sánh các phương thức hình ảnh chính được sử dụng để chụp ảnh xơ vữa động mạch trong bối cảnh thử nghiệm lâm sàng và đánh giá khả năng dự đoán kết quả lâm sàng của chúng đối với một kích thước mẫu nhất định. Chúng tôi xem xét cách các kỹ thuật hình ảnh phân tử và tế bào mới nổi có thể cung cấp khả năng định lượng các thay đổi về chức năng sinh học ở cấp độ mảng bám, ngay cả khi không có thay đổi cấu trúc tổng thể."} +{"idx": 1960071, "en": "The assembly of large and complex organelles, such as the bacterial flagellum, poses the formidable problem of coupling temporal gene expression to specific stages of the organelle-assembly process. The discovery that levels of the bacterial flagellar regulatory protein FlgM are controlled by its secretion from the cell in response to the completion of an intermediate flagellar structure (the hook-basal body) was only the first of several discoveries of unique mechanisms that coordinate flagellar gene expression with assembly. In this Review, we discuss this mechanism, together with others that also coordinate gene regulation and flagellar assembly in Gram-negative bacteria.", "gemini": "Việc lắp ráp các bào quan lớn và phức tạp, chẳng hạn như roi của vi khuẩn, đặt ra vấn đề khó khăn là ghép nối sự biểu hiện gen tạm thời với các giai đoạn cụ thể của quá trình lắp ráp bào quan. Phát hiện ra rằng mức độ của protein điều hòa roi của vi khuẩn FlgM được kiểm soát bởi quá trình tiết ra khỏi tế bào để đáp ứng với sự hoàn thành của cấu trúc roi trung gian (thân móc-gốc) chỉ là phát hiện đầu tiên trong số một số cơ chế độc đáo phối hợp biểu hiện gen roi với lắp ráp. Trong bài Đánh giá này, chúng tôi thảo luận về cơ chế này, cùng với những cơ chế khác cũng phối hợp điều hòa gen và lắp ráp roi ở vi khuẩn Gram âm."} +{"idx": 1960072, "en": "The embryonic programme 'epithelial-mesenchymal transition' (EMT) is thought to promote malignant tumour progression. The transcriptional repressor zinc-finger E-box binding homeobox 1 (ZEB1) is a crucial inducer of EMT in various human tumours, and was recently shown to promote invasion and metastasis of tumour cells. Here, we report that ZEB1 directly suppresses transcription of microRNA-200 family members miR-141 and miR-200c, which strongly activate epithelial differentiation in pancreatic, colorectal and breast cancer cells. Notably, the EMT activators transforming growth factor beta2 and ZEB1 are the predominant targets downregulated by these microRNAs. These results indicate that ZEB1 triggers an microRNA-mediated feedforward loop that stabilizes EMT and promotes invasion of cancer cells. Alternatively, depending on the environmental trigger, this loop might switch and induce epithelial differentiation, and thus explain the strong intratumorous heterogeneity observed in many human cancers.", "gemini": "Chương trình phôi 'chuyển đổi biểu mô-trung mô' (EMT) được cho là thúc đẩy tiến triển của khối u ác tính. Hộp kìm kẹp E-box liên kết kẽm ngón tay 1 (ZEB1) là một chất gây cảm ứng quan trọng của EMT trong nhiều khối u ở người và gần đây đã được chứng minh là thúc đẩy sự xâm lấn và di căn của các tế bào khối u. Ở đây, chúng tôi báo cáo rằng ZEB1 trực tiếp ức chế phiên mã của các thành viên họ microRNA-200 miR-141 và miR-200c, kích hoạt mạnh mẽ sự biệt hóa biểu mô ở các tế bào ung thư tuyến tụy, đại trực tràng và vú. Đáng chú ý, các chất hoạt hóa EMT biến đổi yếu tố tăng trưởng beta2 và ZEB1 là các mục tiêu chính bị ức chế bởi các microRNA này. Những kết quả này chỉ ra rằng ZEB1 kích hoạt một vòng phản hồi tiến tiếp do microRNA trung gian giúp ổn định EMT và thúc đẩy sự xâm lấn của các tế bào ung thư. Ngoài ra, tùy thuộc vào tác nhân kích hoạt môi trường, vòng lặp này có thể chuyển đổi và gây ra sự biệt hóa biểu mô, do đó giải thích tính không đồng nhất mạnh trong khối u được quan sát thấy ở nhiều loại ung thư ở người."} +{"idx": 1960073, "en": "The positive transcription elongation factor b (P-TEFb) is a heterodimeric complex composed of cyclin-dependent kinase 9 and its regulator cyclin T1/2. It stimulates transcription elongation by phosphorylation of serine 2 residues in the carboxy-terminal domain of polymerase II. 7SK RNA and HEXIM proteins can antagonize transcriptional stimulation by sequestering P-TEFb in a catalytically inactive ribonucleoprotein (RNP). Here, we show that the previously uncharacterized La-related protein 7 (LARP7) has a role in 7SK-mediated regulation of transcription. LARP7 binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA and is an integral part of the 7SK RNP. On stimulation, LARP7 remains associated with 7SK RNA, whereas P-TEFb is released. Interestingly, reduction of LARP7 by RNA interference enhances transcription from cellular polymerase II promoters, as well as a TAT-dependent HIV-1 promoter. Thus, LARP7 is a negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system.", "gemini": "Yếu tố kéo dài phiên mã dương tính b (P-TEFb) là một phức hợp dị hợp tử bao gồm kinase phụ thuộc cyclin 9 và chất điều hòa cyclin T1/2 của nó. Nó kích thích kéo dài phiên mã bằng cách phosphoryl hóa các gốc serine 2 trong miền đầu cuối carboxyl của polymerase II. Protein 7SK RNA và HEXIM có thể đối kháng sự kích thích phiên mã bằng cách cô lập P-TEFb trong một ribonucleoprotein (RNP) không hoạt động về mặt xúc tác. Ở đây, chúng tôi chỉ ra rằng protein 7 liên quan đến La (LARP7) trước đây chưa được mô tả có vai trò trong quá trình điều hòa phiên mã do 7SK trung gian. LARP7 liên kết với đoạn U giàu 3' được bảo tồn cao của 7SK RNA và là một phần không thể thiếu của 7SK RNP. Khi được kích thích, LARP7 vẫn liên kết với 7SK RNA, trong khi P-TEFb được giải phóng. Điều thú vị là, việc giảm LARP7 bằng cách can thiệp RNA làm tăng phiên mã từ các promotor polymerase II của tế bào, cũng như một promotor HIV-1 phụ thuộc TAT. Do đó, LARP7 là một chất điều hòa phiên mã âm tính của các gen polymerase II, hoạt động thông qua hệ thống 7SK RNP."} +{"idx": 1960074, "en": "Programmed cell death or apoptosis is required for the patterning and development of multicellular organisms. However, apoptosis is a difficult process to measure because the dead cells are rapidly degraded by their neighbors within a few hours. The post-caspase activation events that determine whether a cell will undergo apoptosis remain elusive. Here we report that apoptosis-specific nuclear events that occur before DNA fragmentation can be distinguished by monitoring the histone H1 status. In both mammals and Drosophila, dying cells failed to be immunolabeled with an anti-H1 monoclonal antibody, AE-4. Real-time imaging of caspase activation and H1 dynamics in mammalian neural cells revealed that H1 changed its location in the nucleus after caspase activation. In addition, the timing of this re-localization was largely dependent on the apoptotic stimulus used. From the staining patterns of AE-4 and anti-active caspase-3 antibodies, cells undergoing the transition from caspase activation to the apoptotic H1 change could be identified as H1-positive caspase-activated cells, providing a novel criterion for early apoptosis and making it possible to characterize caspase-activated cells in tissues. On the basis of these staining patterns, we found that many olfactory sensory neurons in the developing mouse olfactory epithelium showed sustained caspase activity without the H1 change, suggesting a unique caspase function in these neurons.", "gemini": "Cái chết tế bào đã lập trình hoặc apoptosis là cần thiết cho quá trình tạo mẫu và phát triển của các sinh vật đa bào. Tuy nhiên, apoptosis là một quá trình khó đo lường vì các tế bào chết bị phân hủy nhanh chóng bởi các tế bào lân cận trong vòng vài giờ. Các sự kiện kích hoạt sau caspase quyết định xem một tế bào có trải qua quá trình apoptosis hay không vẫn còn khó nắm bắt. Ở đây, chúng tôi báo cáo rằng các sự kiện hạt nhân đặc hiệu của apoptosis xảy ra trước khi phân mảnh DNA có thể được phân biệt bằng cách theo dõi trạng thái histone H1. Ở cả động vật có vú và Drosophila, các tế bào chết không được dán nhãn miễn dịch bằng kháng thể đơn dòng kháng H1, AE-4. Hình ảnh thời gian thực về quá trình kích hoạt caspase và động lực H1 trong các tế bào thần kinh có vú cho thấy H1 đã thay đổi vị trí của nó trong nhân sau khi caspase được kích hoạt. Ngoài ra, thời gian tái định vị này phụ thuộc phần lớn vào kích thích apoptosis được sử dụng. Từ các kiểu nhuộm của kháng thể AE-4 và kháng caspase-3 hoạt động, các tế bào trải qua quá trình chuyển đổi từ kích hoạt caspase sang thay đổi H1 của apoptosis có thể được xác định là các tế bào hoạt hóa caspase dương tính với H1, cung cấp một tiêu chí mới cho quá trình apoptosis sớm và giúp có thể đặc trưng các tế bào hoạt hóa caspase trong mô. Trên cơ sở các kiểu nhuộm này, chúng tôi thấy rằng nhiều tế bào thần kinh cảm giác khứu giác trong biểu mô khứu giác của chuột đang phát triển cho thấy hoạt động caspase liên tục mà không có sự thay đổi H1, cho thấy một chức năng caspase độc đáo ở các tế bào thần kinh này."} +{"idx": 1960075, "en": "Apoptosis plays a crucial role in brain development by ensuring that only appropriately growing, migrating, and synapse-forming neurons and their associated glial cells survive. This process involves an intimate relationship between cell-cell interactions and developmental cues and is further impacted by environmental stress during neurogenesis and disease. Oligodendrocytes (OLs), the major myelin-forming cells in the central nervous system, largely form after this wave of neurogenesis but also show a selective vulnerability to cell death stimuli depending on their stage of development. This can affect not only embryonic and early postnatal brain formation but also the response to demyelinating pathologies. In the present review, we discuss the stage-specific sensitivity of OL lineage cells to damage-induced death and how this might impact myelin survival and regeneration during injury or disease.", "gemini": "Chết rụng tế bào đóng vai trò quan trọng trong quá trình phát triển não bằng cách đảm bảo chỉ những tế bào thần kinh phát triển, di chuyển và hình thành khớp thần kinh thích hợp cùng với các tế bào thần kinh đệm liên quan mới tồn tại. Quá trình này liên quan đến mối quan hệ chặt chẽ giữa các tương tác tế bào-tế bào và các tín hiệu phát triển, đồng thời cũng bị ảnh hưởng bởi căng thẳng môi trường trong quá trình sinh thần kinh và bệnh tật. Các tế bào ít gai (OL), là các tế bào tạo myelin chính trong hệ thần kinh trung ương, phần lớn hình thành sau đợt sinh thần kinh này nhưng cũng biểu hiện tính dễ bị tổn thương chọn lọc đối với các kích thích gây chết tế bào tùy thuộc vào giai đoạn phát triển của chúng. Điều này không chỉ có thể ảnh hưởng đến quá trình hình thành não ở giai đoạn phôi và sau sinh sớm mà còn ảnh hưởng đến phản ứng đối với các bệnh lý mất myelin. Trong bài tổng quan hiện tại, chúng tôi thảo luận về độ nhạy cảm theo giai đoạn của các tế bào dòng OL đối với cái chết do tổn thương gây ra và cách thức mà điều này có thể ảnh hưởng đến sự sống sót và tái tạo myelin trong quá trình chấn thương hoặc bệnh tật."} +{"idx": 1960076, "en": "The epidermis, the outer layer of the skin composed of keratinocytes, is a stratified epithelium that functions as a barrier to protect the organism from dehydration and external insults. The epidermis develops depending on the transcription factor p63, a member of the p53 family of transcription factors. p63 is strongly expressed in the innermost basal layer where epithelial cells with high clonogenic and proliferative capacity reside. Deletion of p63 in mice results in a dramatic loss of all keratinocytes and loss of stratified epithelia, probably due to a premature proliferative rundown of the stem and transient amplifying cells. Here we report that microRNA (miR)-203 is induced in vitro in primary keratinocytes in parallel with differentiation. We found that miR-203 specifically targets human and mouse p63 3'-UTRs and not SOCS-3, despite bioinformatics alignment between miR-203 and SOCS-3 3'-UTR. We also show that miR-203 overexpression in proliferating keratinocytes is not sufficient to induce full epidermal differentiation in vitro. In addition, we demonstrate that miR-203 is downregulated during the epithelial commitment of embryonic stem cells, and that overexpression of miR-203 in rapidly proliferating human primary keratinocytes significantly reduces their clonogenic capacity. The results suggest that miR-203, by regulating the DeltaNp63 expression level, is a key molecule controlling the p63-dependent proliferative potential of epithelial precursor cells both during keratinocyte differentiation and in epithelial development. In addition, we have shown that miR-203 can regulate DeltaNp63 levels upon genotoxic damage in head and neck squamous cell carcinoma cells, thus controlling cell survival.", "gemini": "Lớp biểu bì, lớp ngoài của da được tạo thành từ các tế bào sừng, là một biểu mô phân tầng có chức năng như một hàng rào bảo vệ cơ thể khỏi tình trạng mất nước và các tác nhân gây hại bên ngoài. Lớp biểu bì phát triển tùy thuộc vào yếu tố phiên mã p63, một thành viên của họ yếu tố phiên mã p53. p63 được biểu hiện mạnh ở lớp đáy trong cùng, nơi cư trú các tế bào biểu mô có khả năng sinh bản sao và tăng sinh cao. Việc xóa p63 ở chuột dẫn đến mất đột ngột tất cả các tế bào sừng và mất biểu mô phân tầng, có thể là do sự suy giảm tăng sinh sớm của các tế bào gốc và các tế bào khuếch đại tạm thời. Ở đây chúng tôi báo cáo rằng microRNA (miR)-203 được gây ra trong ống nghiệm ở các tế bào sừng nguyên phát song song với quá trình biệt hóa. Chúng tôi phát hiện ra rằng miR-203 nhắm mục tiêu cụ thể vào 3'-UTR của p63 ở người và chuột chứ không phải SOCS-3, mặc dù có sự liên kết sinh tin học giữa miR-203 và SOCS-3 3'-UTR. Chúng tôi cũng chỉ ra rằng sự biểu hiện quá mức miR-203 ở các tế bào sừng đang tăng sinh là không đủ để gây ra sự biệt hóa hoàn toàn của biểu bì trong ống nghiệm. Ngoài ra, chúng tôi chứng minh rằng miR-203 bị giảm điều hòa trong quá trình cam kết biểu mô của các tế bào gốc phôi và sự biểu hiện quá mức của miR-203 ở các tế bào sừng nguyên phát của người đang tăng sinh nhanh chóng làm giảm đáng kể khả năng sinh bản sao của chúng. Kết quả cho thấy rằng miR-203, bằng cách điều chỉnh mức biểu hiện DeltaNp63, là một phân tử chính kiểm soát tiềm năng tăng sinh phụ thuộc p63 của các tế bào tiền thân biểu mô trong quá trình biệt hóa tế bào sừng và phát triển biểu mô. Ngoài ra, chúng tôi đã chỉ ra rằng miR-203 có thể điều chỉnh mức DeltaNp63 sau tổn thương gen độc ở các tế bào ung thư biểu mô vảy đầu và cổ, do đó kiểm soát sự sống còn của tế bào."} +{"idx": 1960077, "en": "Striated skeletal is subject to nonlethal cycles of atrophy in response to a variety of physiological and pathological stimuli, including: starvation, disuse, denervation and inflammation. These cells can also undergo cell death in response to appropriate developmental signals or specific pathological insults. Most of the insights gained into the control of vertebrate skeletal muscle atrophy and death have resulted from experimental interventions rather than natural processes. In contrast, the intersegmental muscles (ISMs) of moths are giant cells that initiate sequential and distinct programs of atrophy and death at the end of metamorphosis as a normal component of development. This model has provided fundamental information about the control, biochemistry, molecular biology and anatomy of naturally occurring atrophy and death in vivo. The ISMs have provided a good complement to studies in vertebrates and may provide insights into clinically relevant disorders.", "gemini": "Cơ vân có vạch chịu các chu kỳ teo cơ không gây tử vong để đáp ứng với nhiều kích thích sinh lý và bệnh lý, bao gồm: đói, không sử dụng, cắt thần kinh và viêm. Các tế bào này cũng có thể trải qua quá trình chết tế bào để đáp ứng với các tín hiệu phát triển thích hợp hoặc các tác nhân gây bệnh lý cụ thể. Hầu hết các hiểu biết thu được về quá trình kiểm soát teo cơ vân và tử vong ở động vật có xương sống đều bắt nguồn từ các can thiệp thực nghiệm chứ không phải từ các quá trình tự nhiên. Ngược lại, các cơ liên đốt (ISM) của bướm đêm là các tế bào khổng lồ khởi phát các chương trình teo cơ và tử vong tuần tự và riêng biệt vào cuối quá trình biến thái như một thành phần bình thường của quá trình phát triển. Mô hình này đã cung cấp thông tin cơ bản về quá trình kiểm soát, sinh hóa, sinh học phân tử và giải phẫu của quá trình teo cơ và tử vong tự nhiên xảy ra trong cơ thể sống. Các ISM đã cung cấp sự bổ sung tốt cho các nghiên cứu ở động vật có xương sống và có thể cung cấp hiểu biết sâu sắc về các rối loạn có liên quan về mặt lâm sàng."} +{"idx": 1960078, "en": "PURPOSE To determine whether multipurpose solutions, widely used for contact lens disinfections, could be at the origin of ocular pathologies (contact lens intolerance and ocular infections). METHODS An observational cohort study (questionnaire analysis) was carried out to estimate the number of contact lens wearers, type of infection, and type of lens care regimen used by patients. Besides, multipurpose solutions cytotoxicity (necrosis and apoptosis) was evaluated on a conjunctival cell line using cytofluorometry. RESULTS In the general population, 59% of contact lens wearers use multipurpose solutions whereas 35% use oxidative products. Of the questioned contact lens wearers with ocular infections, 80% used multipurpose solutions. Multipurpose solutions are therefore not efficient enough against microorganisms, and cannot be considered as disinfectant solutions but only as preservatives. However, preservatives are known to be toxic to ocular surface, so apoptosis induced by multipurpose solutions could lead to ocular surface diseases. Our cytofluorometry study allowed us to demonstrate that contact lens multipurpose solutions containing preservatives are cytotoxic through caspase 3 induction, chromatin condensation and P2X7 cell-death receptor activation, in contrast with unpreserved sterile saline solutions that were found inert. CONCLUSIONS Multipurpose solutions seem to be preservative but not disinfecting solutions. They are not adapted to the final rinse of contact lenses because of apoptosis induction. It could explain part of lens intolerance.", "gemini": "MỤC ĐÍCH Xác định xem các dung dịch đa năng, được sử dụng rộng rãi để khử trùng kính áp tròng, có thể là nguyên nhân gây ra các bệnh lý về mắt (không dung nạp kính áp tròng và nhiễm trùng mắt) hay không. PHƯƠNG PHÁP Một nghiên cứu theo dõi nhóm (phân tích bảng câu hỏi) đã được thực hiện để ước tính số người đeo kính áp tròng, loại nhiễm trùng và loại chế độ chăm sóc kính áp tròng mà bệnh nhân sử dụng. Bên cạnh đó, độc tính tế bào của các dung dịch đa năng (hoại tử và chết theo chương trình) đã được đánh giá trên dòng tế bào kết mạc bằng phương pháp đo lưu lượng tế bào. KẾT QUẢ Trong dân số nói chung, 59% người đeo kính áp tròng sử dụng dung dịch đa năng trong khi 35% sử dụng các sản phẩm oxy hóa. Trong số những người đeo kính áp tròng được hỏi có nhiễm trùng mắt, 80% sử dụng dung dịch đa năng. Do đó, các dung dịch đa năng không đủ hiệu quả để chống lại vi sinh vật và không thể được coi là dung dịch khử trùng mà chỉ là chất bảo quản. Tuy nhiên, chất bảo quản được biết là có độc đối với bề mặt mắt, vì vậy quá trình chết theo chương trình do các dung dịch đa năng gây ra có thể dẫn đến các bệnh về bề mặt mắt. Nghiên cứu đo lưu lượng tế bào của chúng tôi cho phép chúng tôi chứng minh rằng các dung dịch đa năng cho kính áp tròng có chứa chất bảo quản có độc tính tế bào thông qua quá trình cảm ứng caspase 3, ngưng tụ chromatin và hoạt hóa thụ thể chết tế bào P2X7, ngược lại với các dung dịch nước muối vô trùng không chứa chất bảo quản được tìm thấy là trơ. KẾT LUẬN Các dung dịch đa năng có vẻ như là chất bảo quản nhưng không phải là dung dịch khử trùng. Chúng không thích hợp để rửa sạch kính áp tròng vì gây ra quá trình chết theo chương trình. Điều này có thể giải thích một phần tình trạng không dung nạp kính áp tròng."} +{"idx": 1960079, "en": "PURPOSE To investigate the incidence of asystole during strabismus surgery. DESIGN Retrospective, noncomparative interventional case series. METHODS Six months to 80 years of age of 3628 consecutive patients who underwent strabismus surgery from October 1994 to May 2007 were enrolled. RESULTS Four patients (0.11%) under general anaesthesia showed asystole during strabismus surgery. All four were adults of 28, 32, 50, and 53 years of age. Two patients had hypertension and three had preoperative electrocardiographic abnormalities. Three patients had previously undergone uneventful strabismus surgery. CONCLUSIONS Asystole is likely to be encountered during strabismus surgery with an incidence of ca. 0.11%.", "gemini": "MỤC ĐÍCH Để điều tra tỷ lệ ngừng tim trong quá trình phẫu thuật lác. THIẾT KẾ Nghiên cứu ca can thiệp hồi cứu, không so sánh. PHƯƠNG PHÁP Sáu tháng đến 80 tuổi của 3628 bệnh nhân liên tiếp đã phẫu thuật lác từ tháng 10 năm 1994 đến tháng 5 năm 2007 đã được tuyển dụng. KẾT QUẢ Bốn bệnh nhân (0,11%) dưới gây mê toàn thân đã bị ngừng tim trong quá trình phẫu thuật lác. Cả bốn đều là người lớn từ 28, 32, 50 và 53 tuổi. Hai bệnh nhân bị tăng huyết áp và ba bệnh nhân có bất thường điện tâm đồ trước phẫu thuật. Ba bệnh nhân trước đó đã trải qua phẫu thuật lác mà không có biến cố nào. KẾT LUẬN Có khả năng gặp phải tình trạng ngừng tim trong quá trình phẫu thuật lác với tỷ lệ khoảng 0,11%."} +{"idx": 1960080, "en": "AIM To evaluate the effectiveness of vitreous surgery with direct central retinal artery massage for the treatment of central retinal artery occlusion (CRAO). METHODS Vitreous surgery with direct central retinal artery massage was performed on 10 consecutive patients with acute CRAO. After standard 3 port pars plana vitrectomy, a specially designed probe was used to massage the central retinal artery on the optic nerve head or within the optic nerve or both. The best-corrected visual acuity was measured and fundus photograph was taken before operation, at 24, 48 h and weekly intervals for at least 1 month postoperatively. RESULTS Circulation was restored immediately during the operation in four cases, gradually since the first day after operation in four cases. There was no change in the remaining two cases, among which, central retinal vein occlusion occurred in one case 5 days later. No other complications occurred. At 2 months postoperatively, visual acuity had improved for three or more lines in six cases (60%), and remained the same in the rest of the cases. CONCLUSIONS Vitreous surgery with direct central retinal artery massage seems to be an effective and relatively safe treatment for CRAO", "gemini": "MỤC ĐÍCH Đánh giá hiệu quả của phẫu thuật dịch kính kết hợp xoa bóp trực tiếp động mạch trung tâm võng mạc trong điều trị tắc động mạch trung tâm võng mạc (CRAO). PHƯƠNG PHÁP Phẫu thuật dịch kính kết hợp xoa bóp trực tiếp động mạch trung tâm võng mạc được thực hiện trên 10 bệnh nhân liên tiếp bị CRAO cấp tính. Sau khi cắt dịch kính ba cổng tiêu chuẩn, một đầu dò được thiết kế đặc biệt đã được sử dụng để xoa bóp động mạch trung tâm võng mạc trên đầu dây thần kinh thị giác hoặc bên trong dây thần kinh thị giác hoặc cả hai. Đo thị lực được hiệu chỉnh tốt nhất và chụp ảnh đáy mắt trước khi phẫu thuật, lúc 24, 48 giờ và các khoảng thời gian hàng tuần trong ít nhất 1 tháng sau phẫu thuật. KẾT QUẢ Tuần hoàn được phục hồi ngay trong khi phẫu thuật ở bốn trường hợp, dần dần từ ngày đầu tiên sau phẫu thuật ở bốn trường hợp. Không có thay đổi nào ở hai trường hợp còn lại, trong đó có một trường hợp bị tắc tĩnh mạch trung tâm võng mạc sau 5 ngày. Không có biến chứng nào khác xảy ra. Ở thời điểm 2 tháng sau phẫu thuật, thị lực đã cải thiện ở ba dòng trở lên ở sáu trường hợp (60%) và vẫn giữ nguyên ở các trường hợp còn lại. KẾT LUẬN Phẫu thuật dịch kính kết hợp xoa bóp trực tiếp động mạch trung tâm võng mạc có vẻ như là một phương pháp điều trị hiệu quả và tương đối an toàn cho CRAO"} +{"idx": 1960081, "en": "Mast cells can promote inflammation and other tissue changes in IgE-associated allergic disorders, as well as in certain innate and adaptive immune responses that are thought to be independent of IgE. However, mast cells can also have anti-inflammatory and immunosuppressive functions. Here, we review the evidence that mast cells can have negative, as well as positive, immunomodulatory roles in vivo, and we propose that mast cells can both enhance and later suppress certain features of an immune response.", "gemini": "Các tế bào mast có thể thúc đẩy tình trạng viêm và các thay đổi khác của mô trong các rối loạn dị ứng liên quan đến IgE, cũng như trong một số đáp ứng miễn dịch bẩm sinh và thích ứng được cho là không phụ thuộc vào IgE. Tuy nhiên, các tế bào mast cũng có thể có chức năng chống viêm và ức chế miễn dịch. Ở đây, chúng tôi xem xét bằng chứng cho thấy các tế bào mast có thể có vai trò điều hòa miễn dịch tiêu cực cũng như tích cực trong cơ thể sống và chúng tôi đề xuất rằng các tế bào mast có thể vừa tăng cường vừa ức chế một số đặc điểm nhất định của phản ứng miễn dịch."} +{"idx": 1960082, "en": "Our past research has focused on identifying an effective carrier composed of histidine and lysine for delivery of nucleic acid into cells. For this purpose, we developed histidine-lysine-rich (HK) polymers with specific sequences and branching. We have found that branched HK polymers in complex with Raf-1 siRNA markedly decreased Raf-1 mRNA and induced apoptosis in cell lines in vitro. The primary focus of the present study was to determine an effective carrier to deliver siRNA systemically to tumor xenografts. After comparing HK:Raf-1 polyplexes for their in-vivo efficacy, we investigated in greater detail whether one of these polymers, H3K(+H)4b, in complex with Raf-1 siRNA, inhibited the growth of MDA-MB-435 xenografts. H3K(+H)4b is a four-branched HK peptide whose predominant repeating sequence within the terminal arm is -HHHK-. After the first tail-vein injection in a mouse model, there was a statistically significant reduction in tumor size between the H3K(+H)4b:Raf-1 siRNA-treated and the control groups (P<0.01). By the third injection, there was nearly a 50% reduction in the Raf-1 siRNA-treated group compared to the control siRNA-treated or -untreated group. Consistent with a significant effect of the HK:Raf-1 polyplex on the tumor, there were marked histological changes, increased apoptosis and fewer vessels in the Raf-1 siRNA-treated group. Raf-1 protein within the tumor was significantly decreased after treatment with the HK:Raf-1 siRNA polyplex compared to the control treatment groups. Despite the striking effect on the tumor by the HK Raf-1 siRNA, there was little evidence of toxicity in normal tissues with this therapy. By harnessing the ability to modify the amino-acid sequence and branching of HK polymers, we expect continued development of HK polymers as in-vivo carriers of siRNA.", "gemini": "Nghiên cứu trước đây của chúng tôi tập trung vào việc xác định một chất mang hiệu quả bao gồm histidine và lysine để đưa axit nucleic vào tế bào. Với mục đích này, chúng tôi đã phát triển các polyme histidine-lysine giàu (HK) với các trình tự và nhánh cụ thể. Chúng tôi đã phát hiện ra rằng các polyme HK phân nhánh phức hợp với siRNA Raf-1 làm giảm đáng kể mRNA Raf-1 và gây apoptosis ở các dòng tế bào trong ống nghiệm. Mục tiêu chính của nghiên cứu hiện tại là xác định một chất mang hiệu quả để đưa siRNA vào khối u ghép dị loại một cách toàn thân. Sau khi so sánh các phức hợp HK:Raf-1 về hiệu quả trong cơ thể sống, chúng tôi đã nghiên cứu chi tiết hơn xem một trong những polyme này, H3K(+H)4b, phức hợp với siRNA Raf-1, có ức chế sự phát triển của khối u ghép dị loại MDA-MB-435 hay không. H3K(+H)4b là một peptide HK bốn nhánh có trình tự lặp lại chiếm ưu thế trong nhánh đầu cuối là -HHHK-. Sau lần tiêm tĩnh mạch đầu tiên ở mô hình chuột, đã có sự giảm đáng kể về mặt thống kê về kích thước khối u giữa nhóm được điều trị bằng siRNA H3K(+H)4b:Raf-1 và nhóm đối chứng (P < 0,01). Đến lần tiêm thứ ba, đã có sự giảm gần 50% ở nhóm được điều trị bằng siRNA Raf-1 so với nhóm được điều trị bằng siRNA đối chứng hoặc không được điều trị. Phù hợp với tác dụng đáng kể của phức hợp HK:Raf-1 đối với khối u, đã có những thay đổi mô học rõ rệt, tăng apoptosis và ít mạch máu hơn ở nhóm được điều trị bằng siRNA Raf-1. Protein Raf-1 trong khối u giảm đáng kể sau khi điều trị bằng phức hợp siRNA HK:Raf-1 so với các nhóm điều trị đối chứng. Mặc dù siRNA HK Raf-1 có tác dụng đáng kể đối với khối u, nhưng có rất ít bằng chứng về độc tính ở các mô bình thường với liệu pháp này. Bằng cách tận dụng khả năng sửa đổi trình tự axit amin và nhánh của polyme HK, chúng tôi kỳ vọng sẽ tiếp tục phát triển các polyme HK như chất mang siRNA trong cơ thể sống."} +{"idx": 1960083, "en": "We investigated the effects of p21(waf1/cip1) gene overexpression in human laryngeal squamous carcinoma cells HEp-2 lacking p53 protein expression on apoptosis induction upon the treatment with two commonly used chemotherapeutic agents, cisplatin and methotrexate. For that purpose, we employed cDNA arrays and qPCR to monitor gene expression upon treatment with AdCMV-p21 alone or in combination with the chemotherapeutic compounds. We found that p21(waf1/cip1) gene overexpression provoked apoptosis of HEp-2 through the induction of the TNFRSF9 gene and activation of caspase 7. In addition, we have proved that p21(waf1/cip1) can assume a dual role in apoptosis in the same cell system depending on the chemotherapeutic agent: its overexpression enhances apoptosis in cisplatin-treated cells and attenuates apoptotic signals in methotrexate-treated cells. The observed dual role of p21(waf1/cip1) was in direct correlation with the modulation of caspases 3 and 7 activation and changes in the expression of GADD45a gene. The results presented herein encourage future use of targeted p21(waf1/cip1) gene therapy in cancer treatment in a well-defined therapeutic and genetic context.", "gemini": "Chúng tôi đã nghiên cứu các tác động của sự biểu hiện quá mức gen p21(waf1/cip1) ở các tế bào ung thư biểu mô vảy thanh quản của người HEp-2 thiếu biểu hiện protein p53 khi gây apoptosis sau khi điều trị bằng hai loại thuốc hóa trị thường dùng là cisplatin và methotrexate. Với mục đích đó, chúng tôi đã sử dụng mảng cDNA và qPCR để theo dõi biểu hiện gen sau khi điều trị bằng AdCMV-p21 đơn lẻ hoặc kết hợp với các hợp chất hóa trị. Chúng tôi thấy rằng sự biểu hiện quá mức của gen p21(waf1/cip1) gây ra apoptosis của HEp-2 thông qua quá trình gây ra gen TNFRSF9 và kích hoạt caspase 7. Ngoài ra, chúng tôi đã chứng minh rằng p21(waf1/cip1) có thể đảm nhận vai trò kép trong quá trình apoptosis ở cùng một hệ thống tế bào tùy thuộc vào tác nhân hóa trị: sự biểu hiện quá mức của nó làm tăng apoptosis ở các tế bào được điều trị bằng cisplatin và làm giảm các tín hiệu apoptosis ở các tế bào được điều trị bằng methotrexate. Vai trò kép được quan sát của p21(waf1/cip1) có mối tương quan trực tiếp với sự điều hòa hoạt hóa caspase 3 và 7 và những thay đổi trong biểu hiện của gen GADD45a. Các kết quả được trình bày ở đây khuyến khích sử dụng liệu pháp gen p21(waf1/cip1) được nhắm mục tiêu trong tương lai để điều trị ung thư trong bối cảnh di truyền và điều trị được xác định rõ."} +{"idx": 1960084, "en": "IgA class switching is the process whereby B cells acquire the expression of IgA, the most abundant antibody isotype in mucosal secretions. IgA class switching occurs via both T-cell-dependent and T-cell-independent pathways, and the antibody targets both pathogenic and commensal microorganisms. This Review describes recent advances indicating that innate immune recognition of microbial signatures at the epithelial-cell barrier is central to the selective induction of mucosal IgA class switching. In addition, the mechanisms of IgA class switching at follicular and extrafollicular sites within the mucosal environment are summarized. A better understanding of these mechanisms may help in the development of more effective mucosal vaccines.", "gemini": "Chuyển đổi lớp IgA là quá trình mà tế bào B có được biểu hiện IgA, loại kháng thể phong phú nhất trong dịch tiết niêm mạc. Chuyển đổi lớp IgA xảy ra thông qua cả con đường phụ thuộc tế bào T và không phụ thuộc tế bào T, và kháng thể nhắm vào cả vi sinh vật gây bệnh và cộng sinh. Tổng quan này mô tả những tiến bộ gần đây chỉ ra rằng sự nhận diện miễn dịch bẩm sinh các dấu hiệu của vi khuẩn tại hàng rào tế bào biểu mô là trung tâm của quá trình cảm ứng chọn lọc chuyển đổi lớp IgA niêm mạc. Ngoài ra, các cơ chế chuyển đổi lớp IgA tại các vị trí nang và ngoại nang trong môi trường niêm mạc được tóm tắt. Hiểu rõ hơn về các cơ chế này có thể giúp phát triển vắc-xin niêm mạc hiệu quả hơn."} +{"idx": 1960085, "en": "Selective gene transfer to tumor is critical in cancer gene therapy. We previously used ionizing radiation to improve adenovirus uptake in intrahepatic tumors but liver cytotoxicity associated with the viral administration still occurred. Here, we explore the potential of radiation for improving gene delivery by a virus-mimicking nanoparticle, transferrin (Tf)-cationic liposome-DNA complex (Tf-lipoplex). Transduction by Tf-lipoplex was highly efficient in various cell lines and further increased by radiation in a dose- and time-dependent manner. This radiation induction, which was associated with an increase in Tf-lipoplex uptake (3- to 4-folds in hepatocytes WB and lung cancer cells, LLC1), was absent when a Tf-deficient complex was used or abolished by the presence of free Tf, suggesting that Tf receptor (TfR) interaction is required for radiation induction. Radiation (10-20 Gy) markedly induced transgene (LacZ) expression in LLC1 xenografts (3.5- to 7.4-folds), correlating with increased plasmid content and TfR expression in irradiated tumors. Moreover, Tf-lipoplex-mediated gene expression was not observed in the liver or other normal tissues regardless of radiation treatment. We conclude that radiation improves Tf-lipoplex gene delivery selectively to tumor cells both in vitro and in vivo. Our findings may provide insight in developing ligand-specific lipoplex for molecularly targeted cancer gene therapy.", "gemini": "Truyền gen chọn lọc vào khối u rất quan trọng trong liệu pháp gen ung thư. Trước đây, chúng tôi đã sử dụng bức xạ ion hóa để cải thiện sự hấp thụ adenovirus trong các khối u trong gan nhưng độc tính với gan liên quan đến việc sử dụng virus vẫn xảy ra. Tại đây, chúng tôi khám phá tiềm năng của bức xạ trong việc cải thiện việc đưa gen vào bằng một hạt nano bắt chước virus, phức hợp DNA-liposome cation-transferrin (Tf) (Tf-lipoplex). Quá trình chuyển nạp gen bằng Tf-lipoplex có hiệu quả cao ở nhiều dòng tế bào khác nhau và tăng thêm nữa nhờ bức xạ theo cách phụ thuộc vào liều và thời gian. Sự cảm ứng bức xạ này, liên quan đến sự gia tăng hấp thu Tf-lipoplex (3 đến 4 lần ở tế bào gan WB và tế bào ung thư phổi, LLC1), không có khi sử dụng phức hợp thiếu Tf hoặc bị loại bỏ bởi sự hiện diện của Tf tự do, cho thấy tương tác thụ thể Tf (TfR) là cần thiết cho cảm ứng bức xạ. Bức xạ (10-20 Gy) gây ra biểu hiện transgene (LacZ) rõ rệt ở khối ghép dị loài LLC1 (3,5 đến 7,4 lần), tương quan với hàm lượng plasmid và biểu hiện TfR tăng lên trong các khối u được chiếu xạ. Hơn nữa, biểu hiện gen do Tf-lipoplex trung gian không được quan sát thấy ở gan hoặc các mô bình thường khác bất kể điều trị bằng bức xạ. Chúng tôi kết luận rằng bức xạ cải thiện việc đưa gen Tf-lipoplex một cách chọn lọc vào các tế bào khối u cả trong ống nghiệm và trong cơ thể sống. Những phát hiện của chúng tôi có thể cung cấp thông tin chi tiết trong việc phát triển lipoplex đặc hiệu với phối tử cho liệu pháp gen ung thư nhắm mục tiêu phân tử."} +{"idx": 1960086, "en": "An instrument is demonstrated that is capable of three-dimensional (3D) vasculature imaging and pO(2) quantification with high spatial resolution. The instrument combines two-photon (2P) microscopy with phosphorescence quenching to measure pO(2). The instrument was demonstrated by performing depth-resolved microvascular pO(2) measurements of rat cortical vessels down to 120 microm below the surface. 2P excitation of porphyrin was confirmed, and measured pO(2) values were consistent with previously published data for normoxic and hyperoxic conditions. The ability to perform 3D pO(2) measurements using optical techniques will allow researchers to overcome existing limitations imposed by polarographic electrodes, magnetic resonance techniques, and surface-only pO(2) measurement techniques.", "gemini": "Một dụng cụ được chứng minh có khả năng tạo ảnh mạch máu ba chiều (3D) và định lượng pO(2) với độ phân giải không gian cao. Dụng cụ này kết hợp kính hiển vi hai photon (2P) với sự dập tắt phát quang để đo pO(2). Dụng cụ này đã được chứng minh bằng cách thực hiện các phép đo pO(2) vi mạch có độ sâu của các mạch máu vỏ não của chuột xuống đến 120 microm dưới bề mặt. Sự kích thích 2P của porphyrin đã được xác nhận và các giá trị pO(2) được đo được phù hợp với dữ liệu đã công bố trước đó đối với các điều kiện normoxic và hyperoxic. Khả năng thực hiện các phép đo pO(2) 3D bằng các kỹ thuật quang học sẽ cho phép các nhà nghiên cứu vượt qua những hạn chế hiện tại do các điện cực phân cực, kỹ thuật cộng hưởng từ và các kỹ thuật đo pO(2) chỉ trên bề mặt áp đặt."} +{"idx": 1960087, "en": "We study the combined effects of dynamic gain saturation and two-photon absorption on the amplification of short pulses in semiconductor optical amplifiers and show that two-photon absorption can saturate the amplifier gain and limit the output pulse energies even for amplifiers with large gain saturation energies. We discuss the upper limits for the pulse energies obtainable from semiconductor optical amplifiers in the presence of two-photon absorption and show that for single transverse mode waveguide amplifiers these upper limits can range from values as small as a few picojoules to several hundred picojoules for pulse widths in the 0.5 ps to 20 ps range, respectively.", "gemini": "Chúng tôi nghiên cứu các hiệu ứng kết hợp của độ bão hòa độ lợi động và hấp thụ hai photon đối với sự khuếch đại các xung ngắn trong các bộ khuếch đại quang bán dẫn và chỉ ra rằng sự hấp thụ hai photon có thể làm bão hòa độ lợi của bộ khuếch đại và giới hạn năng lượng xung đầu ra ngay cả đối với các bộ khuếch đại có năng lượng bão hòa độ lợi lớn. Chúng tôi thảo luận về các giới hạn trên đối với năng lượng xung có thể thu được từ các bộ khuếch đại quang bán dẫn khi có sự hấp thụ hai photon và chỉ ra rằng đối với các bộ khuếch đại dẫn sóng chế độ ngang đơn, các giới hạn trên này có thể dao động từ các giá trị nhỏ như vài picojoule đến vài trăm picojoule đối với chiều rộng xung trong phạm vi từ 0,5 ps đến 20 ps."} +{"idx": 1960088, "en": "A diode-pumped Q-switched Tm(3+),Ho(3+)-codoped double-clad silica fiber laser is reported providing average powers of up to 12.3 W at 2072.7 nm and stable pulse widths of only 45 ns and repetition rates in the range 10-100 kHz. Instabilities in pulse shape occur at nonoptimum cavity alignment and were found to be linked to the mechanism responsible for self mode locking.", "gemini": "Một laser sợi quang hai lớp phủ được bơm điốt Q được báo cáo cung cấp công suất trung bình lên đến 12,3 W ở bước sóng 2072,7 nm và chiều rộng xung ổn định chỉ 45 ns và tốc độ lặp lại trong phạm vi 10-100 kHz. Sự bất ổn định về hình dạng xung xảy ra ở sự căn chỉnh khoang không tối ưu và được phát hiện là có liên quan đến cơ chế chịu trách nhiệm cho chế độ tự khóa."} +{"idx": 1960089, "en": "We propose a new technique to realize an optical time lens for ultrafast temporal processing that is based on four-wave mixing in a silicon nanowaveguide. The demonstrated time lens produces more than 100 pi of phase shift, which is not readily achievable using electro-optic phase modulators. Using this method we demonstrate 20x magnification of a signal consisting of two 3 ps pulses, which allows for temporal measurements using a detector with a 20 GHz bandwidth. Our technique offers the capability of ultrafast temporal characterization and processing in a chip-scale device.", "gemini": "Chúng tôi đề xuất một kỹ thuật mới để tạo ra thấu kính thời gian quang học cho xử lý thời gian siêu nhanh dựa trên trộn bốn sóng trong một nanodây dẫn sóng silicon. Thấu kính thời gian được chứng minh tạo ra hơn 100 pi độ lệch pha, điều này không dễ đạt được khi sử dụng bộ điều chế pha điện quang. Sử dụng phương pháp này, chúng tôi chứng minh độ phóng đại 20x của tín hiệu bao gồm hai xung 3 ps, cho phép thực hiện các phép đo thời gian bằng máy dò có băng thông 20 GHz. Kỹ thuật của chúng tôi cung cấp khả năng đặc tính và xử lý thời gian siêu nhanh trong một thiết bị có kích thước chip."} +{"idx": 1960090, "en": "We demonstrate a single-core multiply doped fiber that, when pumped with a single pump source of approximately 800 nm, emits a more than two-octaves-spanning fluorescence spectrum ranging from 365 to 2300 nm. The fiber preform is manufactured from granulated oxides, and the core is doped with five different rare earths. At a pump power of 250 mW the total emitted power is 34 microW; given a core diameter of 6.5 microm and a numerical aperture of 0.1, the radiance exceeds 3 kW sr(-1) cm(-2). We also demonstrate direct diode pumping of the fiber.", "gemini": "Chúng tôi chứng minh một sợi quang đa tạp chất lõi đơn, khi được bơm bằng một nguồn bơm đơn khoảng 800 nm, phát ra phổ huỳnh quang kéo dài hơn hai quãng tám từ 365 đến 2300 nm. Sợi tiền chế được sản xuất từ các oxit dạng hạt, và lõi được tạp chất với năm loại đất hiếm khác nhau. Ở công suất bơm là 250 mW, tổng công suất phát ra là 34 microW; với đường kính lõi là 6,5 microm và độ mở số là 0,1, độ rạng vượt quá 3 kW sr(-1) cm(-2). Chúng tôi cũng chứng minh việc bơm diode trực tiếp của sợi."} +{"idx": 1960091, "en": "The singlet excited-state lifetime of a terpyridyl platinum(II) pentynyl complex was determined to be 268+/-87 ps by fitting femtosecond transient absorption data, the triplet excited-state lifetime was found to be 62 ns by fitting nanosecond transient absorption decay data, and the triplet quantum yield was measured to be 0.16. A ground-state absorption cross section of 2.5 x 10(-19) cm(2) at 532 nm was deduced from UV-vis absorption data. Excited-state absorption cross sections of 3.5 x 10(-17) cm(2) (singlet) and 4.5 x 10(-17) cm(2) (triplet) were obtained by using a five-level dynamic model to fit open-aperture Z scans at picosecond and nanosecond pulse widths and a variety of pulse energies.", "gemini": "Tuổi thọ trạng thái kích thích đơn của phức hợp pentynyl terpyridyl bạch kim (II) được xác định là 268+/-87 ps bằng cách khớp dữ liệu hấp thụ thoáng qua femto giây, tuổi thọ trạng thái kích thích ba trạng thái được tìm thấy là 62 ns bằng cách khớp dữ liệu phân rã hấp thụ thoáng qua nano giây và năng suất lượng tử ba trạng thái được đo là 0,16. Mặt cắt hấp thụ trạng thái cơ bản là 2,5 x 10(-19) cm(2) tại 532 nm được suy ra từ dữ liệu hấp thụ UV-vis. Các mặt cắt hấp thụ trạng thái kích thích là 3,5 x 10(-17) cm(2) (trạng thái đơn) và 4,5 x 10(-17) cm(2) (trạng thái ba) thu được bằng cách sử dụng mô hình động năm mức để khớp các phép quét Z khẩu độ mở ở độ rộng xung pico giây và nano giây và nhiều năng lượng xung khác nhau."} +{"idx": 1960092, "en": "We study experimentally and numerically the nonlinear scattering of wave packets by local multisite guiding centers embedded in a continuous dielectric medium as a function of the input power and angle of incidence. The extent of trapping into the linear modes of different sites is manipulated as a function of both the input power and the angle of incidence, demonstrating power-controlled switching of nonlinear trapping by local photonic potentials.", "gemini": "Chúng tôi nghiên cứu theo thực nghiệm và số về sự tán xạ phi tuyến của các gói sóng bởi các trung tâm dẫn hướng đa vị trí cục bộ được nhúng trong môi trường điện môi liên tục như một hàm của công suất đầu vào và góc tới. Mức độ bẫy vào các chế độ tuyến tính của các vị trí khác nhau được điều khiển như một hàm của cả công suất đầu vào và góc tới, chứng minh sự chuyển đổi được điều khiển bằng công suất của bẫy phi tuyến tính bởi các thế quang tử cục bộ."} +{"idx": 1960093, "en": "We have succeeded in achieving independent control of the repetition rate and optical frequency of a pulse laser by employing a regenerative mode-locking technique. By adopting a voltage-controlled microwave phase shifter or an optical delay line in a regenerative feedback loop we can control the repetition rate of the laser without directly disturbing the optical frequencies. We experimentally show how this independent control can be realized by employing a 40 GHz harmonically and regeneratively mode-locked fiber laser.", "gemini": "Chúng tôi đã thành công trong việc đạt được khả năng kiểm soát độc lập tốc độ lặp và tần số quang của một laze xung bằng cách sử dụng kỹ thuật khóa chế độ tái sinh. Bằng cách sử dụng bộ dịch pha vi sóng điều khiển điện áp hoặc đường trễ quang trong vòng phản hồi tái sinh, chúng tôi có thể kiểm soát tốc độ lặp của laze mà không làm nhiễu trực tiếp đến các tần số quang. Chúng tôi trình bày thí nghiệm về cách kiểm soát độc lập này có thể được thực hiện bằng cách sử dụng laze sợi được khóa chế độ tái sinh và điều hòa bậc 40 GHz."} +{"idx": 1960094, "en": "A novel configuration of stationary Fourier transform infrared (FTIR) spectrometer is presented. Contrary to classic configurations, the interferometer is directly integrated in the focal plane array (FPA) during its process of fabrication. A first, to the best of our knowledge, demonstration of the spectrometric function has been achieved departing from a well-known structure of an HgCdTe photodetector. We show that the obtained FTIR-FPA can be described by intrinsic parameters such as an optical path difference and a so-called spectrometric efficiency. First experimental results are presented.", "gemini": "Một cấu hình mới của máy quang phổ hồng ngoại biến đổi Fourier (FTIR) tĩnh được trình bày. Trái ngược với các cấu hình cổ điển, giao thoa kế được tích hợp trực tiếp vào mảng tiêu cự (FPA) trong quá trình chế tạo. Lần đầu tiên, theo hiểu biết của chúng tôi, việc trình diễn chức năng quang phổ đã đạt được xuất phát từ cấu trúc nổi tiếng của máy dò ảnh HgCdTe. Chúng tôi chỉ ra rằng FTIR-FPA thu được có thể được mô tả bằng các thông số nội tại như chênh lệch đường quang và cái được gọi là hiệu suất quang phổ. Các kết quả thử nghiệm đầu tiên được trình bày."} +{"idx": 1960095, "en": "A short-pulse, two-color Yb:fiber laser system has been developed for mid-infrared generation. To date, 20 microW of average power at a wavelength of approximately 18 microm is generated by difference-frequency mixing 300 mW average power from the two-color Yb:fiber amplifier. The mid-infrared power was not limited by two-photon absorption, allowing it to be scaled by increasing the amplifier power.", "gemini": "Một hệ thống laser sợi Yb hai màu xung ngắn đã được phát triển để tạo ra tia hồng ngoại giữa. Đến nay, 20 microW công suất trung bình ở bước sóng khoảng 18 microm được tạo ra bằng cách trộn tần số chênh lệch 300 mW công suất trung bình từ bộ khuếch đại sợi Yb hai màu. Công suất hồng ngoại giữa không bị giới hạn bởi sự hấp thụ hai photon, cho phép nó được mở rộng bằng cách tăng công suất bộ khuếch đại."} +{"idx": 1960096, "en": "We demonstrate high-fidelity optical arbitrary waveform generation with 5 GHz waveform switching via time-domain multiplexing. Compact, integrated waveform shapers based on silica arrayed-waveguide grating pairs with 10 GHz channel spacing are used to shape (line-by-line) two different waveforms from the output of a 10-mode x 10 GHz optical frequency comb generator. Characterization of the time multiplexer's complex transfer function (amplitude and phase) by frequency-resolved optical gating permits compensation of its impact on the switched waveforms and matching of the measured and target waveforms to better than G'=5%.", "gemini": "Chúng tôi trình bày tạo dạng sóng tùy ý quang học có độ trung thực cao với chuyển đổi dạng sóng 5 GHz thông qua ghép kênh miền thời gian. Các bộ định hình dạng sóng tích hợp nhỏ gọn dựa trên các cặp lưới nhiễu xạ hướng dẫn sóng sắp xếp silica với khoảng cách kênh 10 GHz được sử dụng để định hình (từng dòng một) hai dạng sóng khác nhau từ đầu ra của bộ tạo lược tần số quang học 10 chế độ x 10 GHz. Đặc tính của hàm truyền phức tạp của bộ ghép kênh thời gian (biên độ và pha) bằng cách đóng cổng quang có độ phân giải tần số cho phép bù đắp tác động của nó lên các dạng sóng được chuyển đổi và khớp các dạng sóng đo được và dạng sóng mục tiêu tốt hơn G'=5%."} +{"idx": 1960097, "en": "We demonstrate a mode-locked all-normal-dispersion ytterbium-doped fiber laser constructed with polarization-maintaining fibers. Spectral filtering of a chirped pulse in the cavity, along with a semiconductor saturable absorber, produce self-starting femtosecond mode-locked operation with large normal dispersion. Environmentally stable generation of 2 nJ and 300 fs pulses is achieved.", "gemini": "Chúng tôi trình bày một laser sợi pha tạp ytterbium khóa chế độ phân tán hoàn toàn bình thường được chế tạo bằng các sợi duy trì phân cực. Lọc phổ của xung có độ chớp trong khoang, cùng với bộ hấp thụ bão hòa bán dẫn, tạo ra hoạt động khóa chế độ femto giây tự khởi động với độ phân tán bình thường lớn. Đạt được tạo xung 2 nJ và 300 fs ổn định về mặt môi trường."} +{"idx": 1960098, "en": "Based on the perturbative series representation of a complex-source-point spherical wave an expression for cylindrically symmetrical complex-argument Laguerre-Gauss beams of radial order n is derived. This description acquires the accuracy up to any order of diffraction angle, and its first three corrected terms are in accordance with those given by Seshadri [Opt. Lett.27, 1872 (2002)] based on the virtual source method. Numerical results show that on the beam axis the number of orders of nonvanishing nonparaxial corrections is equal to n. Meanwhile a higher radial mode number n leads to a smaller convergent domain of radius.", "gemini": "Dựa trên biểu diễn chuỗi nhiễu loạn của sóng cầu điểm nguồn phức hợp, biểu thức cho chùm tia Laguerre-Gauss đối xứng hình trụ có bậc xuyên tâm n được suy ra. Mô tả này đạt được độ chính xác lên đến bất kỳ bậc nào của góc nhiễu xạ và ba số hạng hiệu chỉnh đầu tiên của nó phù hợp với các số hạng do Seshadri đưa ra [Opt. Lett.27, 1872 (2002)] dựa trên phương pháp nguồn ảo. Kết quả số cho thấy rằng trên trục chùm, số bậc của các hiệu chỉnh không phải trục không biến mất bằng n. Trong khi đó, số chế độ xuyên tâm n cao hơn dẫn đến miền hội tụ bán kính nhỏ hơn."} +{"idx": 1960099, "en": "We present, for the first time based on our knowledge, a novel in-fiber optical phase modulator that is inherently broadband, efficient, and polarization-maintaining (PM). The modulator is a 25 mm long section of a D-shaped cladding PM fiber with half of its silica cladding replaced with an electro-optic (EO) silicone gel. A phase modulation of more than pi rad has been demonstrated. We describe techniques that enable the long-distance exposure of the fiber core, microfabrication of on-fiber electrodes, a low refractive index EO cladding material for replacing silica fiber cladding, and accurate phase measurement utilizing a Sagnac interferometer.", "gemini": "Dựa trên kiến thức của chúng tôi, chúng tôi lần đầu tiên trình bày một bộ điều chế pha quang học trong sợi mới vốn có băng thông rộng, hiệu quả và duy trì phân cực (PM). Bộ điều chế là một đoạn dài 25 mm của sợi PM có lớp vỏ hình chữ D với một nửa lớp vỏ silica được thay thế bằng gel silicon điện quang (EO). Một điều chế pha lớn hơn pi rad đã được chứng minh. Chúng tôi mô tả các kỹ thuật cho phép tiếp xúc từ xa lõi sợi, chế tạo vi điện cực trên sợi, vật liệu lớp vỏ EO có chiết suất thấp để thay thế lớp vỏ sợi silica và đo pha chính xác bằng cách sử dụng giao thoa kế Sagnac."} +{"idx": 1960100, "en": "We apply the screw triangle method (STM), which was conventionally used in the field of mechanism design, to design a prism to produce a specified image orientation change (IOC). Compared to existing methods [Tsai and Lin, Appl. Opt.45, 3951 (2006); Appl. Opt.46, 3087 (2007)], the proposed method is both simpler and more efficient, since its derivations are essentially vector-based calculations. The validity of the proposed approach is demonstrated via an illustrative example.", "gemini": "Chúng tôi áp dụng phương pháp tam giác vít (STM), vốn thường được sử dụng trong lĩnh vực thiết kế cơ chế, để thiết kế lăng kính nhằm tạo ra sự thay đổi hướng ảnh (IOC) được chỉ định. So với các phương pháp hiện có [Tsai và Lin, Appl. Opt.45, 3951 (2006); Appl. Opt.46, 3087 (2007)], phương pháp được đề xuất vừa đơn giản hơn vừa hiệu quả hơn, vì các phép tính của nó về cơ bản là các phép tính dựa trên vectơ. Tính hợp lệ của phương pháp tiếp cận được đề xuất được chứng minh thông qua một ví dụ minh họa."} +{"idx": 1960101, "en": "We show that the degree of light-speed control in a semiconductor optical amplifier can be significantly extended by the introduction of optical filtering. We achieve a phase shift of approximately 150 degrees at 19 GHz modulation frequency, corresponding to a several-fold increase of the absolute phase shift as well as the achievable bandwidth. We show good quantitative agreement with numerical simulations, including the effects of population oscillations and four-wave mixing, and provide a simple physical explanation based on an analytical perturbation approach.", "gemini": "Chúng tôi chỉ ra rằng mức độ kiểm soát tốc độ ánh sáng trong bộ khuếch đại quang bán dẫn có thể được mở rộng đáng kể bằng cách đưa vào bộ lọc quang học. Chúng tôi đạt được độ dịch pha khoảng 150 độ ở tần số điều chế 19 GHz, tương ứng với độ dịch pha tuyệt đối tăng gấp nhiều lần cũng như băng thông có thể đạt được. Chúng tôi chỉ ra sự nhất quán định lượng tốt với các mô phỏng số, bao gồm cả các hiệu ứng của dao động dân số và trộn bốn sóng, và đưa ra một giải thích vật lý đơn giản dựa trên phương pháp nhiễu loạn phân tích."} +{"idx": 1960102, "en": "We report on a single-shot micropatterning of an organic polymer achieved by ablation with demagnifying projection using a Ne-like Zn 21.2 nm soft x-ray laser. A nickel mesh with a period of 100 microm was approximately 10x demagnified and imprinted on poly(methyl methacrylate) via direct ablation. The quality of the ablated microstructure was found to be mainly dependent on the quality of the projected mask. This first demonstration (to our knowledge) of single-shot projection, single-step lithography illustrates the potential of soft x-ray lasers for the direct patterning of materials with a resolution scalable down to submicrometer domain.", "gemini": "Chúng tôi báo cáo về vi mô đơn phát của một polyme hữu cơ đạt được bằng cách cắt bỏ với phép chiếu phóng đại sử dụng tia laser tia X mềm Zn 21,2 nm giống Ne. Một lưới niken có chu kỳ 100 microm đã được phóng đại khoảng 10 lần và in trên poly (methyl methacrylate) thông qua cắt bỏ trực tiếp. Chất lượng của vi cấu trúc bị cắt bỏ được phát hiện chủ yếu phụ thuộc vào chất lượng của mặt nạ được chiếu. Biểu hiện đầu tiên này (theo hiểu biết của chúng tôi) về phép chiếu một phát, quang khắc một bước minh họa tiềm năng của tia laser tia X mềm để tạo mẫu trực tiếp các vật liệu với độ phân giải có thể mở rộng xuống miền dưới micrômet."} +{"idx": 1960103, "en": "Theoretical bit error rates of the differential phase-shift keying format with various regeneration schemes is presented. A comparison with ideal regeneration reveals that averaging the phase noise of adjacent bits is found to eliminate most penalties that are induced by phase noise.", "gemini": "Tỷ lệ lỗi bit lý thuyết của định dạng dịch pha khóa với các chương trình tái tạo khác nhau được trình bày. So sánh với tái tạo lý tưởng cho thấy việc trung bình độ nhiễu pha của các bit liền kề được phát hiện để loại bỏ hầu hết các hình phạt do nhiễu pha gây ra."} +{"idx": 1960104, "en": "A near-field microscope coupled with a near-infrared (NIR) supercontinuum source is developed and applied to characterize optical modes in a three-dimensional (3D) woodpile photonic crystal (PC) possessing a NIR partial bandgap. Spatially resolved near-field intensity distributions under different illumination wavelengths demonstrate that the electric fields preferentially dwell in the polymer rods or in the gaps between rods, respectively, for frequencies below or above the stop gap, as predicted by the 3D finite-difference time-domain modeling. Near-field microspectroscopy further reveals that the position-dependent band-edge effect plays an important role in PC-based all-optical integrated devices.", "gemini": "Một kính hiển vi trường gần kết hợp với nguồn siêu liên tục gần hồng ngoại (NIR) được phát triển và áp dụng để đặc trưng các chế độ quang học trong tinh thể photonic gỗ ba chiều (3D) (PC) sở hữu một dải tần một phần NIR. Phân bố cường độ trường gần được giải quyết không gian dưới các bước sóng chiếu sáng khác nhau chứng minh rằng các trường điện ưu tiên nằm trong các thanh polyme hoặc trong các khoảng trống giữa các thanh, tương ứng, đối với các tần số dưới hoặc trên khoảng dừng, như được dự đoán bởi mô hình miền thời gian hữu hạn 3D. Quang phổ vi trường gần hơn nữa cho thấy rằng hiệu ứng cạnh dải phụ thuộc vào vị trí đóng một vai trò quan trọng trong các thiết bị tích hợp hoàn toàn quang học dựa trên PC."} +{"idx": 1960105, "en": "The spin profile of a fiber is usually optimized to reduce transmission impairments caused by polarization-mode dispersion (PMD). In this paper, we show that fiber-optic-based plug-and-play quantum-key distribution systems using polarization modulation and fibers with a spin profile optimal for PMD may suffer from a large Faraday rotation induced by the geomagnetic field. We show that, for periodic spin patterns of small periods, the Faraday rotation is minimum when no spin is applied to the fiber.", "gemini": "Hồ sơ vòng quay của một sợi quang thường được tối ưu hóa để giảm các khiếm khuyết truyền dẫn gây ra bởi sự phân tán chế độ phân cực (PMD). Trong bài báo này, chúng tôi chỉ ra rằng các hệ thống phân phối khóa lượng tử cắm và chạy dựa trên sợi quang sử dụng điều chế phân cực và các sợi có hồ sơ vòng quay tối ưu cho PMD có thể bị ảnh hưởng bởi sự quay Faraday lớn do từ trường địa từ gây ra. Chúng tôi chỉ ra rằng, đối với các mẫu vòng quay định kỳ có chu kỳ nhỏ, độ quay Faraday là nhỏ nhất khi không có vòng quay nào được áp dụng cho sợi quang."} +{"idx": 1960106, "en": "We demonstrate a novel technique for coupling extreme-ultraviolet (XUV) harmonic radiation out of a femtosecond enhancement cavity. We use a small-period diffraction grating etched directly into the surface of a dielectric mirror. For the fundamental light, this element acts as a high reflector. For harmonic wavelengths, it acts as a diffraction grating, coupling XUV radiation out of the cavity. Using this method, we observed the third through twenty-first odd harmonics with a dramatic increase in usable power over previous results of high-harmonic generation at high repetition rates.", "gemini": "Chúng tôi trình bày một kỹ thuật mới để ghép bức xạ sóng hài cực tím (XUV) ra khỏi một khoang tăng cường femtosecond. Chúng tôi sử dụng một mạng nhiễu xạ chu kỳ nhỏ được khắc trực tiếp vào bề mặt của một gương điện môi. Đối với ánh sáng cơ bản, thành phần này hoạt động như một bộ phản xạ cao. Đối với các bước sóng hài, nó hoạt động như một mạng nhiễu xạ, ghép bức xạ XUV ra khỏi khoang. Sử dụng phương pháp này, chúng tôi đã quan sát thấy từ sóng hài lẻ thứ ba đến thứ hai mươi mốt với sự gia tăng đáng kể về công suất có thể sử dụng so với các kết quả trước đây về tạo sóng hài cao ở tốc độ lặp lại cao."} +{"idx": 1960107, "en": "We demonstrate parabolic optical pulse generation by manipulating the intensity and phase of individual longitudinal modes of a 40 GHz picosecond optical pulse train in the spectral domain. Bright and dark parabolic pulses were generated from a 40 GHz mode-locked fiber laser using a 64-channel arrayed waveguide grating pulse shaper. The obtained parabolic pulse, which can easily generate a linear chirping, is useful for a number of applications to optical signal processing applications, including pulse compression and time-domain optical Fourier transformation.", "gemini": "Chúng tôi trình bày việc tạo xung quang parabolic bằng cách thao tác cường độ và pha của các chế độ dọc riêng lẻ của một chuỗi xung quang picosecond 40 GHz trong miền phổ. Các xung parabolic sáng và tối được tạo ra từ laser sợi khóa chế độ 40 GHz bằng bộ định hình xung mạng dẫn sóng xếp hàng 64 kênh. Xung parabolic thu được, có thể dễ dàng tạo ra một tiếng kêu tuyến tính, hữu ích cho một số ứng dụng xử lý tín hiệu quang, bao gồm nén xung và biến đổi Fourier quang miền thời gian."} +{"idx": 1960108, "en": "A simple refractive index sensor based on a Michelson interferometer in a single-mode fiber is constructed and demonstrated. The sensor consists of a single symmetrically abrupt taper region in a short piece of single-mode fiber that is terminated by approximately 500 nm thick gold coating. The sensitivity of the new sensor is similar to that of a long-period-grating-type sensor, and its ease of fabrication offers a low-cost alternative to current sensing applications.", "gemini": "Một cảm biến chiết suất đơn giản dựa trên giao thoa kế Michelson trong sợi đơn mode được chế tạo và chứng minh. Cảm biến bao gồm một vùng thuôn nhọn đối xứng duy nhất trong một đoạn sợi đơn mode ngắn được kết thúc bằng lớp phủ vàng dày khoảng 500 nm. Độ nhạy của cảm biến mới tương tự như độ nhạy của cảm biến loại mạng dài kỳ và dễ chế tạo, cung cấp một giải pháp thay thế giá rẻ cho các ứng dụng cảm biến hiện tại."} +{"idx": 1960109, "en": "Spectral phase ripple associated with novel dispersive devices can distort broadband optical signals. We present a digital postprocessing algorithm to correct for this distortion by exploiting the static deterministic nature of the ripple. This algorithm is demonstrated with empirical data for several systems employing chirped fiber Bragg gratings (CFBGs). We employ this technique in a photonic time-stretch system incorporating CFBGs, improving the signal fidelity by 9 dB. Simulations and experiments show that this algorithm, which can be reduced to a simple interpolation and matrix multiplication, also mitigates additive noise. We see that the act of distortion correction yields signal fidelity superior to that of an ideal dispersive element.", "gemini": "Gợn pha phổ liên quan đến các thiết bị phân tán mới có thể làm méo tín hiệu quang băng rộng. Chúng tôi trình bày một thuật toán xử lý hậu kỳ kỹ thuật số để hiệu chỉnh độ méo này bằng cách khai thác bản chất xác định tĩnh của gợn. Thuật toán này được chứng minh với dữ liệu thực nghiệm cho một số hệ thống sử dụng mạng quang sợi Bragg có tần số quét (CFBG). Chúng tôi sử dụng kỹ thuật này trong hệ thống kéo dài thời gian quang học kết hợp CFBG, cải thiện độ trung thực của tín hiệu lên 9 dB. Các mô phỏng và thí nghiệm cho thấy thuật toán này, có thể được giảm xuống thành phép nội suy và phép nhân ma trận đơn giản, cũng làm giảm nhiễu cộng. Chúng tôi thấy rằng hành động hiệu chỉnh độ méo mang lại độ trung thực của tín hiệu cao hơn độ trung thực của tín hiệu của một phần tử phân tán lý tưởng."} +{"idx": 1960110, "en": "A novel all-diode-pumped master oscillator power amplifier system based on Yb:YAG crystal rods has been developed. It consists of a Q-switched oscillator delivering 3 mJ, 6.4 ns pulses at a 10 Hz repetition rate and an additional four-pass amplifier, which boosts the output energy to 220 mJ, while a close to TEM(00) beam quality could be observed. Additionally a simulation of the amplification was written that allows for further scaling considerations.", "gemini": "Một hệ thống khuếch đại công suất dao động chính được bơm toàn bộ bằng diode mới dựa trên các thanh tinh thể Yb:YAG đã được phát triển. Nó bao gồm một dao động chuyển đổi Q cung cấp các xung 3 mJ, 6,4 ns ở tốc độ lặp lại 10 Hz và một bộ khuếch đại bốn lần bổ sung, giúp tăng năng lượng đầu ra lên 220 mJ, trong khi có thể quan sát thấy chất lượng chùm gần với TEM(00). Ngoài ra, một mô phỏng khuếch đại đã được viết cho phép xem xét khả năng mở rộng hơn nữa."} +{"idx": 1960111, "en": "The exigencies of high-concentration photovoltaics motivate optics that (1) obviate the need for optical bonds, (2) exhibit maximal optical tolerance, (3) are not damaged at off-axis orientation, and (4) allow convenient location of the solar cell and heat sink. We show that dual-mirror unfolded aplanats can satisfy all these criteria. Lens enhancement improves compactness and, with millimeter-scale cells, concentrator depth is only a few centimeters, amenable to precise large-volume fabrication.", "gemini": "Những yêu cầu cấp thiết của quang điện tập trung cao thúc đẩy quang học (1) loại bỏ nhu cầu về liên kết quang học, (2) thể hiện dung sai quang học tối đa, (3) không bị hư hỏng ở phương ngoài trục và (4) cho phép vị trí thuận tiện của pin mặt trời và tản nhiệt. Chúng tôi chỉ ra rằng các aplanat mở ra gương kép có thể đáp ứng tất cả các tiêu chí này. Cải tiến ống kính giúp cải thiện độ nhỏ gọn và với các ô kích thước milimet, độ sâu của bộ tập trung chỉ là vài cm, phù hợp với chế tạo khối lượng lớn chính xác."} +{"idx": 1960112, "en": "Narrowband localized wave packets that are nondispersing and nondiffracting in one transverse dimension are characterized in anomalously dispersive media by means of a Fourier approach. Depending on the group velocity, waves with a dispersion relationship characterized by real wavenumbers can be O or X waves, while we also find waves with evanescent wavenumbers.", "gemini": "Các gói sóng cục bộ hẹp không phân tán và không nhiễu xạ trong một chiều ngang được đặc trưng trong môi trường phân tán bất thường bằng phương pháp Fourier. Tùy thuộc vào vận tốc nhóm, các sóng có mối quan hệ phân tán được đặc trưng bởi số sóng thực có thể là sóng O hoặc X, trong khi chúng ta cũng tìm thấy các sóng có số sóng biến mất."} +{"idx": 1960113, "en": "We address the properties of two-dimensional surface solitons supported by the interface of a waveguide array whose nonlinearity is periodically modulated. When the nonlinearity strength reaches its minima at the points where the linear refractive index attains its maxima, we found that nonlinear surface waves exist and can be made stable only within a limited band of input energy flows and for lattice depths exceeding a lower threshold.", "gemini": "Chúng tôi giải quyết các đặc tính của soliton bề mặt hai chiều được hỗ trợ bởi giao diện của một mảng ống dẫn sóng có tính phi tuyến được điều chế theo chu kỳ. Khi cường độ phi tuyến đạt giá trị cực tiểu tại các điểm mà chỉ số khúc xạ tuyến tính đạt giá trị cực đại, chúng tôi thấy rằng sóng bề mặt phi tuyến tồn tại và chỉ có thể ổn định trong một dải giới hạn của luồng năng lượng đầu vào và đối với độ sâu mạng lưới vượt quá ngưỡng thấp hơn."} +{"idx": 1960114, "en": "The autocorrelation of a modulated coherent light source is used as a probe pulse in a time-domain interferometry scheme. With respect to conventional techniques, higher flexibility in selecting the shape of the probe pulse can be achieved by simply acting on the modulation parameters. The complex amplitude of short pulses propagating through a generic optical device can be directly measured, with no need for fast sampling and time synchronization. The potentialities of the technique are shown by reporting measurements of amplitude distortion, group delay, and frequency chirp of optical pulses transmitted through integrated ring resonators.", "gemini": "Tự tương quan của một nguồn sáng liên kết được điều chế được sử dụng làm xung thăm dò trong một sơ đồ giao thoa miền thời gian. Đối với các kỹ thuật thông thường, có thể đạt được tính linh hoạt cao hơn trong việc lựa chọn hình dạng của xung thăm dò chỉ bằng cách tác động vào các thông số điều chế. Biên độ phức hợp của các xung ngắn lan truyền qua một thiết bị quang học chung có thể được đo trực tiếp mà không cần lấy mẫu nhanh và đồng bộ thời gian. Tiềm năng của kỹ thuật này được thể hiện bằng cách báo cáo các phép đo về độ méo biên độ, độ trễ nhóm và sự dịch tần của các xung quang truyền qua các cộng hưởng vòng tích hợp."} +{"idx": 1960115, "en": "To be legible in high-ambient light conditions, organic light-emitting-diode displays should be optically designed to have a minimal reflectance without significantly affecting their overall efficiency. We demonstrate the use of an anode consisting of a partially absorbing metal layer and a multilayer distributed Bragg reflector to simultaneously absorb rather than reflect incoming light and to take advantage of a weak microcavity effect in the diode to improve light outcoupling.", "gemini": "Để có thể đọc được trong điều kiện ánh sáng xung quanh cao, màn hình điốt phát sáng hữu cơ phải được thiết kế quang học để có độ phản xạ tối thiểu mà không ảnh hưởng đáng kể đến hiệu suất tổng thể của chúng. Chúng tôi chứng minh việc sử dụng cực dương bao gồm lớp kim loại hấp thụ một phần và phản xạ Bragg phân tán nhiều lớp để đồng thời hấp thụ thay vì phản xạ ánh sáng tới và tận dụng hiệu ứng vi khoang yếu trong điốt để cải thiện sự ghép nối ánh sáng ra."} +{"idx": 1960116, "en": "A wavelength-tuned signal-processing approach is proposed for enabling direct unambiguous temperature measurement in a free-space targeted single-crystal silicon carbide (SiC) temperature sensor. The approach simultaneously exploits the 6H SiC fundamental Sellmeier equation-based wavelength-sensitive refractive index change in combination with the classic temperature-dependent refractive index change and the material thermal-expansion path-length change to encode SiC chip temperature with wavelength. Presently, the technique is useful for fast coarse temperature measurement as demonstrated from room temperature to 1000 degrees C using a 10-peak count wavelength-tuned measurement with a 0.31 nm total wavelength change. This coarse technique can be combined with the previously presented two-wavelength signal-processing temperature measurement approach to simultaneously deliver a wide temperature range and a high-resolution temperature sensor. Applications for the sensor range from power plants to materials processing facilities.", "gemini": "Một phương pháp xử lý tín hiệu được điều chỉnh theo bước sóng được đề xuất để cho phép đo nhiệt độ trực tiếp và không gây nhầm lẫn trong cảm biến nhiệt độ silicon carbide (SiC) tinh thể đơn mục tiêu không gian tự do. Phương pháp này đồng thời khai thác sự thay đổi chỉ số khúc xạ nhạy cảm với bước sóng dựa trên phương trình Sellmeier cơ bản của SiC 6H kết hợp với sự thay đổi chỉ số khúc xạ phụ thuộc nhiệt độ cổ điển và sự thay đổi độ dài đường dẫn do giãn nở nhiệt của vật liệu để mã hóa nhiệt độ chip SiC bằng bước sóng. Hiện tại, kỹ thuật này hữu ích cho phép đo nhiệt độ thô nhanh như đã chứng minh từ nhiệt độ phòng đến 1000 độ C bằng cách sử dụng phép đo điều chỉnh bước sóng đếm 10 đỉnh với tổng thay đổi bước sóng 0,31 nm. Kỹ thuật thô này có thể được kết hợp với phương pháp đo nhiệt độ xử lý tín hiệu hai bước sóng đã trình bày trước đó để đồng thời cung cấp dải nhiệt độ rộng và cảm biến nhiệt độ có độ phân giải cao. Các ứng dụng cho cảm biến này trải rộng từ nhà máy điện đến các cơ sở chế biến vật liệu."} +{"idx": 1960117, "en": "We report the observation of surface solitons in chirped semi-infinite waveguide arrays whose waveguides exhibit exponentially decreasing refractive indices. We show that the power threshold for surface wave formation decreases with an increase of the array chirp and that for sufficiently large chirp values linear surface modes are supported.", "gemini": "Chúng tôi báo cáo quan sát về soliton bề mặt trong các mảng dẫn sóng bán vô hạn có độ chirped có các dẫn sóng thể hiện chiết suất giảm theo cấp số nhân. Chúng tôi chỉ ra rằng ngưỡng công suất để hình thành sóng bề mặt giảm khi độ chirped của mảng tăng và đối với các giá trị độ chirped đủ lớn, các chế độ bề mặt tuyến tính được hỗ trợ."} +{"idx": 1960118, "en": "We report a dual-modal device capable of sequential acquisition of Raman spectroscopy (RS) and optical coherence tomography (OCT) along a common optical axis. The device enhances application of both RS and OCT by precisely guiding RS acquisition with OCT images while also compensating for the lack of molecular specificity in OCT with the biochemical specificity of RS. We characterize the system performance and demonstrate the capability to identify structurally ambiguous features within an OCT image with RS in a scattering phantom, guide acquisition of RS from a localized malignancy in ex vivo breast tissue, and perform in vivo tissue analysis of a scab.", "gemini": "Chúng tôi báo cáo một thiết bị chế độ kép có khả năng thu nhận tuần tự phổ Raman (RS) và cắt lớp quang học (OCT) dọc theo một trục quang chung. Thiết bị này tăng cường ứng dụng của cả RS và OCT bằng cách hướng dẫn chính xác quá trình thu nhận RS bằng hình ảnh OCT đồng thời bù đắp cho việc thiếu đặc hiệu phân tử trong OCT bằng đặc hiệu sinh hóa của RS. Chúng tôi mô tả đặc điểm hiệu suất của hệ thống và chứng minh khả năng xác định các đặc điểm mơ hồ về mặt cấu trúc trong một hình ảnh OCT bằng RS trong một hình ảnh ảo tán xạ, hướng dẫn thu nhận RS từ một khối u ác tính tại chỗ trong mô vú ngoại vi và thực hiện phân tích mô trong cơ thể của một cục ghỉ."} +{"idx": 1960119, "en": "We propose and demonstrate a novel Ge photodetector on silicon-on-insulator based on a junction field effect transistor structure, where the field-effect transistor gate is replaced by a Ge island with no contact on it. Light incident on the Ge switches on the device by altering the conductance of the Si channel through secondary photoconductivity. The device's sensitivity is also enhanced by a vast reduction in parasitic capacitance. In cw measurements, proof-of-concept detectors exhibit up to a 33% change in Si channel conductance by absorbing only 200 nW of power at 1.55 microm. In addition, pulsed response tests have shown that rise times as low as 40 ps can be achieved.", "gemini": "Chúng tôi đề xuất và trình bày một máy dò ảnh Ge mới trên silicon trên chất cách điện dựa trên cấu trúc bóng bán dẫn hiệu ứng trường nối, trong đó cổng bóng bán dẫn hiệu ứng trường được thay thế bằng một hòn đảo Ge không tiếp xúc với nó. Ánh sáng chiếu vào Ge bật thiết bị bằng cách thay đổi độ dẫn của kênh Si thông qua độ dẫn quang thứ cấp. Độ nhạy của thiết bị cũng được tăng cường nhờ giảm đáng kể điện dung ký sinh. Trong các phép đo cw, các máy dò minh chứng cho khái niệm thể hiện sự thay đổi lên đến 33% trong độ dẫn của kênh Si bằng cách chỉ hấp thụ 200 nW công suất ở 1,55 microm. Ngoài ra, các thử nghiệm đáp ứng xung đã chỉ ra rằng có thể đạt được thời gian tăng thấp tới 40 ps."} +{"idx": 1960120, "en": "Passive mode locking of a diode-pumped Yb(3+):KYF(4) laser is demonstrated using a semiconductor saturable absorber mirror. A high-stability, transform-limited pulse train with a repetition rate of 57 MHz is generated. Solitonlike pulses with maximum average output power of 250 mW, minimum pulse duration of 170 fs, and rms time jitter of 360 fs were obtained.", "gemini": "Khóa chế độ thụ động của laser Yb(3+):KYF(4) bơm diode được chứng minh bằng cách sử dụng gương hấp thụ bão hòa bán dẫn. Một chuỗi xung giới hạn biến đổi, độ ổn định cao với tốc độ lặp lại 57 MHz được tạo ra. Các xung giống soliton với công suất đầu ra trung bình tối đa là 250 mW, thời lượng xung tối thiểu là 170 fs và độ lệch thời gian rms là 360 fs đã thu được."} +{"idx": 1960121, "en": "We propose a hybrid multiplexing method for volume holographic correlators based on the combination of orthogonal random phase modulation of an object beam and two-dimensional angular multiplexing of a reference beam. This method implements storing multiple groups of library images in a single storage location and outputting multiple groups of correlation spots by switching the modulation phase masks. Three experiments are designed to test the validity of this method.", "gemini": "Chúng tôi đề xuất một phương pháp ghép kênh lai cho bộ tương quan ảnh ba chiều dựa trên sự kết hợp giữa điều chế pha ngẫu nhiên trực giao của chùm vật và ghép kênh góc hai chiều của chùm tham chiếu. Phương pháp này thực hiện việc lưu trữ nhiều nhóm ảnh thư viện trong một vị trí lưu trữ duy nhất và xuất ra nhiều nhóm điểm tương quan bằng cách chuyển đổi mặt nạ pha điều chế. Ba thí nghiệm được thiết kế để kiểm tra tính hợp lệ của phương pháp này."} +{"idx": 1960122, "en": "Two object-dependent filters (an amplitude and a phase filter) are used in the object plane on the iterative calculation of a kinoform instead of a single (phase) filter as usual. The amplitude filter is a system of weight coefficients that vary in the process of iterations and control the amplitude of an input object. The advantages of the proposed method over other ones are confirmed by computer-based experiments. It is found that the method is most efficient for binary objects.", "gemini": "Hai bộ lọc phụ thuộc vào vật thể (bộ lọc biên độ và bộ lọc pha) được sử dụng trong mặt phẳng vật thể trên phép tính lặp của kinoform thay vì một bộ lọc (pha) duy nhất như thông thường. Bộ lọc biên độ là một hệ số trọng số thay đổi trong quá trình lặp và kiểm soát biên độ của một vật thể đầu vào. Những ưu điểm của phương pháp được đề xuất so với các phương pháp khác được xác nhận bằng các thí nghiệm trên máy tính. Người ta thấy rằng phương pháp này hiệu quả nhất đối với các đối tượng nhị phân."} +{"idx": 1960123, "en": "Single-mode lasing from a coupled asymmetric microcavity is achieved. By coupling two size mismatched circular microrings to form a coupled asymmetric microcavity, multi-whispering-gallery modes are successfully suppressed and single-frequency laser emission is robustly obtained. Moreover, the laser emits in four directions, and each beam has a divergence of only 6.6 degrees . It is demonstrated further that this single-frequency coupled microcavity laser can be easily integrated with planar lightwave circuits. We provide an easily accessible approach to achieve a single-frequency laser from microcavity lasers operating on whispering-gallery modes.", "gemini": "Đạt được phát xạ laser một chế độ từ một vi hốc bất đối xứng được ghép nối. Bằng cách ghép nối hai vòng tròn vi kích thước không khớp để tạo thành một vi hốc bất đối xứng được ghép nối, các chế độ phòng thì thầm đa thành công bị triệt tiêu và phát xạ laser tần số đơn được thu được một cách mạnh mẽ. Hơn nữa, laser phát ra theo bốn hướng và mỗi chùm chỉ lệch 6,6 độ. Hơn nữa, chứng minh rằng laser vi hốc ghép tần số đơn này có thể dễ dàng tích hợp với các mạch sóng ánh sáng phẳng. Chúng tôi cung cấp một phương pháp dễ tiếp cận để đạt được laser tần số đơn từ laser vi hốc hoạt động trên các chế độ phòng thì thầm."} +{"idx": 1960124, "en": "We demonstrate rapid fabrication of submicrometer-diameter pores in borosilicate glass using femtosecond laser machining and subsequent wet-etch techniques. This approach allows direct and repeatable fabrication of high-quality pores with diameters of 400-800 nm. Such small pores coupled with the desirable electrical and chemical properties of glass enable sensitive resistive-pulse analysis to determine the size and concentration of macromolecules and nanoparticles. Plasma-enhanced chemical vapor deposition allows further reduction of pore diameters to below 300 nm.", "gemini": "Chúng tôi trình bày chế tạo nhanh các lỗ có đường kính dưới micrômet trong thủy tinh borosilicate bằng cách sử dụng gia công laser femtosecond và các kỹ thuật khắc ướt tiếp theo. Phương pháp này cho phép chế tạo trực tiếp và lặp lại các lỗ có chất lượng cao với đường kính 400-800 nm. Các lỗ nhỏ như vậy kết hợp với các tính chất điện và hóa học mong muốn của thủy tinh cho phép phân tích xung điện trở nhạy để xác định kích thước và nồng độ của các phân tử vĩ mô và hạt nano. Sự lắng đọng hơi hóa học được tăng cường bằng plasma cho phép giảm đường kính lỗ xuống dưới 300 nm."} +{"idx": 1960125, "en": "A dual-imaging modality is demonstrated for high-resolution quantitative imaging of local cerebral blood flow in the rat cortex by combining simultaneous spectral-domain Doppler optical coherence tomography (SDOCT) and full-field laser speckle contrast imaging (LSCI). Preliminary studies in tissue flow phantom and cocaine-induced cerebral blood flow changes indicated that by correlating coregistered cortical arterial blood flow, the relative measurement of flow changes by LSCI could be accurately calibrated by the absolute flow imaging provided by SDOCT (least square fit, r(2) approximately 0.96). Quantitative LSCI of cerebral blood flow is crucial to the quantitative analyses of the spatiotemporal hemodynamics of functional brain activations and thus improved understanding of neural process.", "gemini": "Một phương thức hình ảnh kép được chứng minh để chụp ảnh định lượng độ phân giải cao về lưu lượng máu não cục bộ ở vỏ não chuột bằng cách kết hợp chụp cắt lớp quang học Doppler miền phổ đồng thời (SDOCT) và hình ảnh tương phản đốm laser trường đầy đủ (LSCI). Các nghiên cứu sơ bộ về mô hình lưu lượng mô và những thay đổi lưu lượng máu não do cocaine gây ra chỉ ra rằng bằng cách liên quan đến lưu lượng máu động mạch vỏ não được đăng ký đồng thời, phép đo tương đối về những thay đổi lưu lượng bằng LSCI có thể được hiệu chuẩn chính xác bằng hình ảnh lưu lượng tuyệt đối do SDOCT cung cấp (phù hợp ít nhất, r (2) khoảng 0,96). LSCI định lượng lưu lượng máu não rất quan trọng đối với các phân tích định lượng về huyết động học không gian thời gian của hoạt động chức năng của não và do đó hiểu rõ hơn về quá trình thần kinh."} +{"idx": 1960126, "en": "We recently introduced matched filtering in the context of astronomical Shack-Hartmann wavefront sensing with elongated sodium laser beacons [Appl. Opt. 45, 6568 (2006)]. Detailed wave optics Monte Carlo simulations implementing this technique for the Thirty Meter Telescope dual conjugate adaptive optics system have, however, revealed frequent bursts of degraded closed loop residual wavefront error [Proc. SPIE 6272, 627236 (2006)]. The origin of this problem is shown to be related to laser guide star jitter on the sky that kicks the filter out of its linear dynamic range, which leads to bursts of nonlinearities that are reconstructed into higher-order wavefront aberrations, particularly coma and trifoil for radially elongated subaperture spots. An elegant reformulation of the algorithm is proposed to extend its dynamic range using a set of linear constraints while preserving its improved noise rejection and Monte Carlo performance results are reported that confirm the benefits of the method.", "gemini": "Gần đây, chúng tôi đã giới thiệu bộ lọc khớp trong bối cảnh cảm biến mặt sóng Shack-Hartmann thiên văn với các đèn hiệu laser natri kéo dài [Appl. Opt. 45, 6568 (2006)]. Tuy nhiên, các mô phỏng Monte Carlo quang học sóng chi tiết triển khai kỹ thuật này cho hệ thống quang thích ứng liên hợp kép của Kính thiên văn ba mươi mét đã phát hiện ra các vụ nổ thường xuyên của lỗi mặt sóng dư vòng kín bị suy giảm [Proc. SPIE 6272, 627236 (2006)]. Nguồn gốc của vấn đề này được chứng minh là liên quan đến độ rung của ngôi sao dẫn hướng laser trên bầu trời, làm cho bộ lọc thoát khỏi phạm vi động tuyến tính của nó, dẫn đến các vụ nổ phi tuyến tính được tái tạo thành quang sai mặt sóng bậc cao hơn, đặc biệt là hôn mê và ba lá cho các điểm phụ khẩu kéo dài theo bán kính. Một sự cải tiến thuật toán thanh lịch được đề xuất để mở rộng phạm vi động của nó bằng cách sử dụng một tập hợp các ràng buộc tuyến tính trong khi vẫn giữ nguyên khả năng chống nhiễu được cải thiện và các kết quả hiệu suất Monte Carlo được báo cáo để xác nhận những lợi ích của phương pháp này."} +{"idx": 1960127, "en": "Vector ticks possess a unique system that enables them to digest large amounts of host blood and to transmit various animal and human pathogens, suggesting the existence of evolutionally acquired proteolytic mechanisms. We report here the molecular and reverse genetic characterization of a multifunctional cysteine protease, longipain, from the babesial parasite vector tick Haemaphysalis longicornis. Longipain shares structural similarity with papain-family cysteine proteases obtained from invertebrates and vertebrates. Endogenous longipain was mainly expressed in the midgut epithelium and was specifically localized at lysosomal vacuoles and possibly released into the lumen. Its expression was up-regulated by host blood feeding. Enzymatic functional assays using in vitro and in vivo substrates revealed that longipain hydrolysis occurs over a broad range of pH and temperature. Haemoparasiticidal assays showed that longipain dose-dependently killed tick-borne Babesia parasites, and its babesiacidal effect occurred via specific adherence to the parasite membranes. Disruption of endogenous longipain by RNA interference revealed that longipain is involved in the digestion of the host blood meal. In addition, the knockdown ticks contained an increased number of parasites, suggesting that longipain exerts a killing effect against the midgut-stage Babesia parasites in ticks. Our results suggest that longipain is essential for tick survival, and may have a role in controlling the transmission of tick-transmittable Babesia parasites.", "gemini": "Ve vectơ sở hữu một hệ thống độc đáo cho phép chúng tiêu hóa một lượng lớn máu vật chủ và truyền nhiều loại mầm bệnh ở động vật và người, cho thấy sự tồn tại của các cơ chế phân giải protein có được theo quá trình tiến hóa. Chúng tôi báo cáo ở đây đặc điểm phân tử và di truyền ngược của một protease cysteine đa chức năng, longipain, từ ve ký sinh trùng Babesia Haemaphysalis longicornis. Longipain có cấu trúc tương tự với các protease cysteine họ papain thu được từ động vật không xương sống và động vật có xương sống. Longipain nội sinh chủ yếu được biểu hiện ở biểu mô ruột giữa và được định vị cụ thể tại các túi lysosome và có thể được giải phóng vào lòng ống. Sự biểu hiện của nó được điều chỉnh tăng lên khi ăn máu vật chủ. Các xét nghiệm chức năng bằng enzym sử dụng chất nền trong ống nghiệm và trong cơ thể sống cho thấy quá trình thủy phân longipain xảy ra trong phạm vi pH và nhiệt độ rộng. Các xét nghiệm tiêu diệt ký sinh trùng máu cho thấy longipain giết chết ký sinh trùng Babesia do ve truyền theo liều lượng phụ thuộc, và tác dụng tiêu diệt ký sinh trùng của nó xảy ra thông qua sự bám dính cụ thể vào màng ký sinh trùng. Sự phá vỡ longipain nội sinh bằng can thiệp RNA cho thấy longipain tham gia vào quá trình tiêu hóa bữa ăn máu của vật chủ. Ngoài ra, những con ve bị hạ gục có số lượng ký sinh trùng tăng lên, cho thấy longipain có tác dụng tiêu diệt ký sinh trùng Babesia giai đoạn ruột giữa ở ve. Kết quả của chúng tôi cho thấy longipain rất cần thiết cho sự sống còn của ve và có thể đóng vai trò trong việc kiểm soát quá trình truyền ký sinh trùng Babesia có thể truyền qua ve."} +{"idx": 1960128, "en": "Patients with valvulopathy have the highest risk to develop infective endocarditis (IE), although the relationship between valvulopathy and IE is not clearly understood. Q fever endocarditis, an IE due to Coxiella burnetii, is accompanied by immune impairment. Patients with valvulopathy exhibited increased levels of circulating apoptotic leukocytes, as determined by the measurement of active caspases and nucleosome determination. The binding of apoptotic cells to monocytes and macrophages, the hosts of C. burnetii, may be responsible for the immune impairment observed in Q fever endocarditis. Apoptotic lymphocytes (AL) increased C. burnetii replication in monocytes and monocyte-derived macrophages in a cell-contact dependent manner, as determined by quantitative PCR and immunofluorescence. AL binding induced a M2 program in monocytes and macrophages stimulated with C. burnetii as determined by a cDNA chip containing 440 arrayed sequences and functional tests, but this program was in part different in monocytes and macrophages. While monocytes that had bound AL released high levels of IL-10 and IL-6, low levels of TNF and increased CD14 expression, macrophages that had bound AL released high levels of TGF-beta1 and expressed mannose receptor. The neutralization of IL-10 and TGF-beta1 prevented the replication of C. burnetii due to the binding of AL, suggesting that they were critically involved in bacterial replication. In contrast, the binding of necrotic cells to monocytes and macrophages led to C. burnetii killing and typical M1 polarization. Finally, interferon-gamma corrected the immune deactivation induced by apoptotic cells: it prevented the replication of C. burnetii and re-directed monocytes and macrophages toward a M1 program, which was deleterious for C. burnetii. We suggest that leukocyte apoptosis associated with valvulopathy may be critical for the pathogenesis of Q fever endocarditis by deactivating immune cells and creating a favorable environment for bacterial persistence.", "gemini": "Bệnh nhân mắc bệnh van tim có nguy cơ mắc bệnh viêm nội tâm mạc nhiễm trùng (IE) cao nhất, mặc dù mối quan hệ giữa bệnh van tim và IE vẫn chưa được hiểu rõ. Viêm nội tâm mạc do sốt Q, một loại IE do Coxiella burnetii gây ra, đi kèm với tình trạng suy giảm miễn dịch. Bệnh nhân mắc bệnh van tim biểu hiện mức bạch cầu chết theo chương trình tăng lên, được xác định bằng cách đo các caspase hoạt động và xác định nucleosome. Sự gắn kết của các tế bào chết theo chương trình với các tế bào đơn nhân và đại thực bào, vật chủ của C. burnetii, có thể chịu trách nhiệm cho tình trạng suy giảm miễn dịch được quan sát thấy trong viêm nội tâm mạc do sốt Q. Tế bào lympho chết theo chương trình (AL) làm tăng sinh C. burnetii ở các tế bào đơn nhân và đại thực bào có nguồn gốc từ tế bào đơn nhân theo cách phụ thuộc vào tiếp xúc tế bào, được xác định bằng PCR định lượng và miễn dịch huỳnh quang. Liên kết AL gây ra chương trình M2 ở các tế bào đơn nhân và đại thực bào được kích thích với C. burnetii như được xác định bằng chip cDNA chứa 440 trình tự sắp xếp và các xét nghiệm chức năng, nhưng chương trình này ở một phần khác nhau ở các tế bào đơn nhân và đại thực bào. Trong khi các tế bào đơn nhân đã liên kết AL giải phóng nồng độ IL-10 và IL-6 cao, nồng độ TNF thấp và biểu hiện CD14 tăng lên, các đại thực bào đã liên kết AL giải phóng nồng độ TGF-beta1 cao và thụ thể mannose biểu hiện. Việc trung hòa IL-10 và TGF-beta1 ngăn chặn sự sinh sôi của C. burnetii do liên kết AL, cho thấy chúng có liên quan chặt chẽ đến sự sinh sôi của vi khuẩn. Ngược lại, sự liên kết của các tế bào hoại tử với các tế bào đơn nhân và đại thực bào dẫn đến tiêu diệt C. burnetii và phân cực M1 điển hình. Cuối cùng, interferon-gamma đã hiệu chỉnh tình trạng bất hoạt miễn dịch do các tế bào chết theo chương trình gây ra: nó ngăn chặn sự sinh sôi của C. burnetii và tái định hướng các tế bào đơn nhân và đại thực bào theo chương trình M1, có hại cho C. burnetii. Chúng tôi cho rằng apoptosis bạch cầu liên quan đến bệnh van tim có thể rất quan trọng đối với bệnh sinh của viêm nội tâm mạc do sốt Q bằng cách vô hiệu hóa các tế bào miễn dịch và tạo ra môi trường thuận lợi cho vi khuẩn tồn tại."} +{"idx": 1960129, "en": "The pseudorabies virus (PRV) Us9 protein plays a central role in targeting viral capsids and glycoproteins to axons of dissociated sympathetic neurons. As a result, Us9 null mutants are defective in anterograde transmission of infection in vivo. However, it is unclear how Us9 promotes axonal sorting of so many viral proteins. It is known that the glycoproteins gB, gC, gD and gE are associated with lipid raft microdomains on the surface of infected swine kidney cells and monocytes, and are directed into the axon in a Us9-dependent manner. In this report, we determined that Us9 is associated with lipid rafts, and that this association is critical to Us9-mediated sorting of viral structural proteins. We used infected non-polarized and polarized PC12 cells, a rat pheochromocytoma cell line that acquires many of the characteristics of sympathetic neurons in the presence of nerve growth factor (NGF). In these cells, Us9 is highly enriched in detergent-resistant membranes (DRMs). Moreover, reducing the affinity of Us9 for lipid rafts inhibited anterograde transmission of infection from sympathetic neurons to epithelial cells in vitro. We conclude that association of Us9 with lipid rafts is key for efficient targeting of structural proteins to axons and, as a consequence, for directional spread of PRV from pre-synaptic to post-synaptic neurons and cells of the mammalian nervous system.", "gemini": "Protein Us9 của virus pseudorabies (PRV) đóng vai trò trung tâm trong việc nhắm mục tiêu vỏ capsid và glycoprotein của virus vào các sợi trục của các tế bào thần kinh giao cảm đã phân ly. Do đó, các đột biến mất Us9 bị khiếm khuyết trong quá trình truyền nhiễm ngược dòng trong cơ thể sống. Tuy nhiên, vẫn chưa rõ Us9 thúc đẩy quá trình phân loại sợi trục của nhiều protein virus như thế nào. Người ta biết rằng các glycoprotein gB, gC, gD và gE liên kết với các vi miền bè lipid trên bề mặt của các tế bào thận lợn và các tế bào đơn nhân bị nhiễm bệnh, và được hướng vào sợi trục theo cách phụ thuộc vào Us9. Trong báo cáo này, chúng tôi xác định rằng Us9 liên kết với bè lipid và sự liên kết này rất quan trọng đối với quá trình phân loại protein cấu trúc của virus do Us9 trung gian. Chúng tôi đã sử dụng các tế bào PC12 không phân cực và phân cực bị nhiễm bệnh, một dòng tế bào u tế bào ưa crôm của chuột cống có được nhiều đặc điểm của các tế bào thần kinh giao cảm khi có yếu tố tăng trưởng thần kinh (NGF). Trong các tế bào này, Us9 được làm giàu rất nhiều trong màng kháng chất tẩy rửa (DRM). Hơn nữa, việc giảm ái lực của Us9 đối với bè lipid đã ức chế quá trình truyền nhiễm ngược dòng từ các tế bào thần kinh giao cảm đến các tế bào biểu mô trong ống nghiệm. Chúng tôi kết luận rằng sự liên kết của Us9 với bè lipid là chìa khóa để nhắm mục tiêu hiệu quả các protein cấu trúc vào các sợi trục và do đó, để lây lan định hướng PRV từ các tế bào thần kinh và tế bào trước synap đến sau synap của hệ thần kinh động vật có vú."} +{"idx": 1960130, "en": "The model plant species Arabidopsis thaliana is successful at colonizing land that has recently undergone human-mediated disturbance. To investigate the prehistoric spread of A. thaliana, we applied approximate Bayesian computation and explicit spatial modeling to 76 European accessions sequenced at 876 nuclear loci. We find evidence that a major migration wave occurred from east to west, affecting most of the sampled individuals. The longitudinal gradient appears to result from the plant having spread in Europe from the east approximately 10,000 years ago, with a rate of westward spread of approximately 0.9 km/year. This wave-of-advance model is consistent with a natural colonization from an eastern glacial refugium that overwhelmed ancient western lineages. However, the speed and time frame of the model also suggest that the migration of A. thaliana into Europe may have accompanied the spread of agriculture during the Neolithic transition.", "gemini": "Loài thực vật mô hình Arabidopsis thaliana thành công trong việc xâm chiếm vùng đất gần đây đã trải qua sự xáo trộn do con người gây ra. Để nghiên cứu sự lây lan thời tiền sử của A. thaliana, chúng tôi đã áp dụng tính toán Bayes gần đúng và mô hình không gian rõ ràng cho 76 mẫu vật châu Âu được giải trình tự tại 876 vị trí hạt nhân. Chúng tôi tìm thấy bằng chứng cho thấy một làn sóng di cư lớn đã xảy ra từ đông sang tây, ảnh hưởng đến hầu hết các cá thể được lấy mẫu. Độ dốc theo chiều dọc dường như là kết quả của việc cây lan rộng ở châu Âu từ phía đông cách đây khoảng 10.000 năm, với tốc độ lan rộng về phía tây là khoảng 0,9 km/năm. Mô hình sóng tiến này phù hợp với sự xâm chiếm tự nhiên từ một nơi trú ẩn băng hà phía đông đã áp đảo các dòng dõi phía tây cổ đại. Tuy nhiên, tốc độ và khung thời gian của mô hình cũng cho thấy rằng quá trình di cư của A. thaliana vào châu Âu có thể đi kèm với sự phát triển của nền nông nghiệp trong quá trình chuyển đổi thời kỳ đồ đá mới."} +{"idx": 1960131, "en": "Although acute lung injury (ALI) is a common complication of severe malaria, little is known about the underlying molecular basis of lung dysfunction. Animal models have provided powerful insights into the pathogenesis of severe malaria syndromes such as cerebral malaria (CM); however, no model of malaria-induced lung injury has been definitively established. This study used bronchoalveolar lavage (BAL), histopathology and gene expression analysis to examine the development of ALI in mice infected with Plasmodium berghei ANKA (PbA). BAL fluid of PbA-infected C57BL/6 mice revealed a significant increase in IgM and total protein prior to the development of CM, indicating disruption of the alveolar-capillary membrane barrier-the physiological hallmark of ALI. In contrast to sepsis-induced ALI, BAL fluid cell counts remained constant with no infiltration of neutrophils. Histopathology showed septal inflammation without cellular transmigration into the alveolar spaces. Microarray analysis of lung tissue from PbA-infected mice identified a significant up-regulation of expressed genes associated with the gene ontology categories of defense and immune response. Severity of malaria-induced ALI varied in a panel of inbred mouse strains, and development of ALI correlated with peripheral parasite burden but not CM susceptibility. Cd36(-/-) mice, which have decreased parasite lung sequestration, were relatively protected from ALI. In summary, parasite burden and CD36-mediated sequestration in the lung are primary determinants of ALI in experimental murine malaria. Furthermore, differential susceptibility of mouse strains to malaria-induced ALI and CM suggests that distinct genetic determinants may regulate susceptibility to these two important causes of malaria-associated morbidity and mortality.", "gemini": "Mặc dù tổn thương phổi cấp tính (ALI) là một biến chứng phổ biến của bệnh sốt rét nặng, nhưng người ta vẫn biết rất ít về cơ sở phân tử tiềm ẩn của rối loạn chức năng phổi. Các mô hình động vật đã cung cấp những hiểu biết sâu sắc về quá trình sinh bệnh của các hội chứng sốt rét nặng như sốt rét não (CM); tuy nhiên, không có mô hình nào về tổn thương phổi do sốt rét gây ra được thiết lập một cách chắc chắn. Nghiên cứu này sử dụng rửa phế quản phế nang (BAL), mô bệnh học và phân tích biểu hiện gen để kiểm tra sự phát triển của ALI ở những con chuột bị nhiễm Plasmodium berghei ANKA (PbA). Dịch BAL của những con chuột C57BL/6 bị nhiễm PbA cho thấy sự gia tăng đáng kể IgM và protein toàn phần trước khi phát triển CM, chỉ ra sự phá vỡ hàng rào màng mao mạch phế nang - đặc điểm sinh lý của ALI. Trái ngược với ALI do nhiễm trùng huyết, số lượng tế bào dịch BAL vẫn không đổi mà không có sự thâm nhiễm của bạch cầu trung tính. Mô bệnh học cho thấy tình trạng viêm vách ngăn mà không có sự di chuyển của tế bào vào các khoang phế nang. Phân tích mảng gen của mô phổi từ những con chuột bị nhiễm PbA đã xác định được sự điều hòa đáng kể các gen biểu hiện liên quan đến các loại gen bảo vệ và đáp ứng miễn dịch. Mức độ nghiêm trọng của ALI do sốt rét gây ra khác nhau ở một nhóm các dòng chuột cận huyết, và sự phát triển của ALI có tương quan với gánh nặng ký sinh trùng ngoại vi nhưng không phải là tình trạng dễ mắc CM. Những con chuột Cd36(-/-), có sự giảm lưu giữ ký sinh trùng trong phổi, được bảo vệ tương đối khỏi ALI. Tóm lại, gánh nặng ký sinh trùng và sự lưu giữ do CD36 trung gian trong phổi là những yếu tố quyết định chính của ALI trong bệnh sốt rét ở chuột thực nghiệm. Hơn nữa, tình trạng dễ mắc bệnh khác nhau của các dòng chuột đối với ALI do sốt rét và CM cho thấy rằng các yếu tố di truyền riêng biệt có thể điều chỉnh tình trạng dễ mắc phải hai nguyên nhân quan trọng này gây ra bệnh tật và tử vong liên quan đến sốt rét."} +{"idx": 1960132, "en": "Infection with the human gastric pathogen Helicobacter pylori is associated with a spectrum of diseases including gastritis, peptic ulcers, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue lymphoma. The cytotoxin-associated gene A (CagA) protein of H. pylori, which is translocated into host cells via a type IV secretion system, is a major risk factor for disease development. Experiments in gastric tissue culture cells have shown that once translocated, CagA activates the phosphatase SHP-2, which is a component of receptor tyrosine kinase (RTK) pathways whose over-activation is associated with cancer formation. Based on CagA's ability to activate SHP-2, it has been proposed that CagA functions as a prokaryotic mimic of the eukaryotic Grb2-associated binder (Gab) adaptor protein, which normally activates SHP-2. We have developed a transgenic Drosophila model to test this hypothesis by investigating whether CagA can function in a well-characterized Gab-dependent process: the specification of photoreceptors cells in the Drosophila eye. We demonstrate that CagA expression is sufficient to rescue photoreceptor development in the absence of the Drosophila Gab homologue, Daughter of Sevenless (DOS). Furthermore, CagA's ability to promote photoreceptor development requires the SHP-2 phosphatase Corkscrew (CSW). These results provide the first demonstration that CagA functions as a Gab protein within the tissue of an organism and provide insight into CagA's oncogenic potential. Since many translocated bacterial proteins target highly conserved eukaryotic cellular processes, such as the RTK signaling pathway, the transgenic Drosophila model should be of general use for testing the in vivo function of bacterial effector proteins and for identifying the host genes through which they function.", "gemini": "Nhiễm khuẩn do vi khuẩn gây bệnh ở dạ dày Helicobacter pylori liên quan đến nhiều bệnh bao gồm viêm dạ dày, loét dạ dày tá tràng, ung thư biểu mô tuyến dạ dày và u lympho mô liên quan đến niêm mạc dạ dày. Protein gen A liên quan đến độc tố tế bào (CagA) của H. pylori, được chuyển vào tế bào vật chủ thông qua hệ thống tiết loại IV, là yếu tố nguy cơ chính gây ra bệnh. Các thí nghiệm trên tế bào nuôi cấy mô dạ dày cho thấy khi được chuyển vào, CagA sẽ kích hoạt phosphatase SHP-2, một thành phần của các thụ thể tyrosine kinase (RTK) có sự hoạt hóa quá mức liên quan đến sự hình thành ung thư. Dựa trên khả năng kích hoạt SHP-2 của CagA, người ta cho rằng CagA hoạt động như một chất bắt chước prokaryotic của protein gắn kết liên quan đến Grb2 (Gab) của eukaryote, vốn thường kích hoạt SHP-2. Chúng tôi đã phát triển một mô hình Drosophila chuyển gen để kiểm tra giả thuyết này bằng cách tìm hiểu xem CagA có thể hoạt động trong một quá trình phụ thuộc Gab đã được biết rõ hay không: sự xác định các tế bào thụ quang trong mắt Drosophila. Chúng tôi chứng minh rằng biểu hiện CagA là đủ để cứu vãn sự phát triển của thụ quang khi không có đồng đẳng Gab của Drosophila, Daughter of Sevenless (DOS). Hơn nữa, khả năng thúc đẩy sự phát triển của thụ quang của CagA đòi hỏi phosphatase SHP-2 Corkscrew (CSW). Những kết quả này cung cấp bằng chứng đầu tiên cho thấy CagA hoạt động như một protein Gab trong mô của một cơ thể và cung cấp thông tin chi tiết về tiềm năng gây ung thư của CagA. Vì nhiều protein vi khuẩn được chuyển vào nhắm vào các quá trình tế bào eukaryote được bảo tồn cao, chẳng hạn như con đường truyền tín hiệu RTK, nên mô hình Drosophila chuyển gen sẽ có tác dụng chung để kiểm tra chức năng trong cơ thể sống của các protein tác dụng của vi khuẩn và để xác định các gen vật chủ mà chúng hoạt động thông qua đó."} +{"idx": 1960133, "en": "Repeat-proteins are made up of near repetitions of 20- to 40-amino acid stretches. These polypeptides usually fold up into non-globular, elongated architectures that are stabilized by the interactions within each repeat and those between adjacent repeats, but that lack contacts between residues distant in sequence. The inherent symmetries both in primary sequence and three-dimensional structure are reflected in a folding landscape that may be analyzed as a quasi-one-dimensional problem. We present a general description of repeat-protein energy landscapes based on a formal Ising-like treatment of the elementary interaction energetics in and between foldons, whose collective ensemble are treated as spin variables. The overall folding properties of a complete \"domain\" (the stability and cooperativity of the repeating array) can be derived from this microscopic description. The one-dimensional nature of the model implies there are simple relations for the experimental observables: folding free-energy (DeltaG(water)) and the cooperativity of denaturation (m-value), which do not ordinarily apply for globular proteins. We show how the parameters for the \"coarse-grained\" description in terms of foldon spin variables can be extracted from more detailed folding simulations on perfectly funneled landscapes. To illustrate the ideas, we present a case-study of a family of tetratricopeptide (TPR) repeat proteins and quantitatively relate the results to the experimentally observed folding transitions. Based on the dramatic effect that single point mutations exert on the experimentally observed folding behavior, we speculate that natural repeat proteins are \"poised\" at particular ratios of inter- and intra-element interaction energetics that allow them to readily undergo structural transitions in physiologically relevant conditions, which may be intrinsically related to their biological functions.", "gemini": "Các protein lặp lại được tạo thành từ các đoạn lặp lại gần của 20-40 axit amin. Các polypeptide này thường gấp lại thành các kiến trúc thuôn dài, không hình cầu được ổn định bởi các tương tác trong mỗi lần lặp lại và giữa các lần lặp lại liền kề, nhưng thiếu các tiếp xúc giữa các phần còn lại trong chuỗi. Các đối xứng vốn có trong cả trình tự chính và cấu trúc ba chiều được phản ánh trong một bối cảnh gấp có thể được phân tích như một bài toán gần như một chiều. Chúng tôi trình bày mô tả chung về các bối cảnh năng lượng protein lặp lại dựa trên cách xử lý tương tự như Ising chính thức về năng lượng tương tác cơ bản trong và giữa các foldon, có thể coi tập hợp chung của chúng là các biến spin. Các đặc tính gấp chung của một \"miền\" hoàn chỉnh (độ ổn định và tính hợp tác của mảng lặp lại) có thể được bắt nguồn từ mô tả vi mô này. Bản chất một chiều của mô hình ngụ ý rằng có các mối quan hệ đơn giản đối với các quan sát thực nghiệm: năng lượng tự do gấp (DeltaG(nước)) và tính hợp tác của quá trình biến tính (giá trị m), vốn thường không áp dụng cho các protein hình cầu. Chúng tôi chỉ ra cách các tham số cho mô tả \"hạt thô\" theo các biến spin foldon có thể được trích xuất từ các mô phỏng gấp chi tiết hơn trên các bối cảnh hoàn hảo. Để minh họa các ý tưởng, chúng tôi trình bày một nghiên cứu trường hợp về họ các protein lặp lại tetratricopeptide (TPR) và liên hệ định lượng các kết quả với các chuyển đổi gấp được quan sát bằng thực nghiệm. Dựa trên hiệu ứng đáng kể mà các đột biến điểm đơn gây ra đối với hành vi gấp được quan sát bằng thực nghiệm, chúng tôi suy đoán rằng các protein lặp lại tự nhiên được \"chuẩn bị\" ở các tỷ lệ cụ thể của năng lượng tương tác giữa các phần tử và bên trong phần tử, cho phép chúng dễ dàng trải qua các chuyển đổi cấu trúc trong các điều kiện có liên quan đến sinh lý, vốn có thể liên quan chặt chẽ đến chức năng sinh học của chúng."} +{"idx": 1960134, "en": "Reconstructions of cellular metabolism are publicly available for a variety of different microorganisms and some mammalian genomes. To date, these reconstructions are \"genome-scale\" and strive to include all reactions implied by the genome annotation, as well as those with direct experimental evidence. Clearly, many of the reactions in a genome-scale reconstruction will not be active under particular conditions or in a particular cell type. Methods to tailor these comprehensive genome-scale reconstructions into context-specific networks will aid predictive in silico modeling for a particular situation. We present a method called Gene Inactivity Moderated by Metabolism and Expression (GIMME) to achieve this goal. The GIMME algorithm uses quantitative gene expression data and one or more presupposed metabolic objectives to produce the context-specific reconstruction that is most consistent with the available data. Furthermore, the algorithm provides a quantitative inconsistency score indicating how consistent a set of gene expression data is with a particular metabolic objective. We show that this algorithm produces results consistent with biological experiments and intuition for adaptive evolution of bacteria, rational design of metabolic engineering strains, and human skeletal muscle cells. This work represents progress towards producing constraint-based models of metabolism that are specific to the conditions where the expression profiling data is available.", "gemini": "Các bản tái tạo quá trình trao đổi chất của tế bào hiện có sẵn công khai cho nhiều loại vi sinh vật khác nhau và một số bộ gen của động vật có vú. Cho đến nay, các bản tái tạo này là \"quy mô bộ gen\" và cố gắng bao gồm tất cả các phản ứng ngụ ý bởi chú thích bộ gen, cũng như các phản ứng có bằng chứng thực nghiệm trực tiếp. Rõ ràng, nhiều phản ứng trong tái tạo quy mô bộ gen sẽ không hoạt động trong các điều kiện cụ thể hoặc trong một loại tế bào cụ thể. Các phương pháp để điều chỉnh các bản tái tạo toàn bộ bộ gen toàn diện này thành các mạng cụ thể theo ngữ cảnh sẽ hỗ trợ mô hình hóa in silico có tính dự đoán cho một tình huống cụ thể. Chúng tôi trình bày một phương pháp có tên là Gene Inactivity Moderated by Metabolism and Expression (GIMME) để đạt được mục tiêu này. Thuật toán GIMME sử dụng dữ liệu biểu hiện gen định lượng và một hoặc nhiều mục tiêu trao đổi chất được giả định trước để tạo ra bản tái tạo cụ thể theo ngữ cảnh phù hợp nhất với dữ liệu có sẵn. Hơn nữa, thuật toán cung cấp điểm số không nhất quán định lượng chỉ ra mức độ nhất quán của một tập hợp dữ liệu biểu hiện gen với một mục tiêu trao đổi chất cụ thể. Chúng tôi chỉ ra rằng thuật toán này tạo ra các kết quả phù hợp với các thí nghiệm sinh học và trực giác về sự tiến hóa thích nghi của vi khuẩn, thiết kế hợp lý các chủng kỹ thuật chuyển hóa và tế bào cơ xương của người. Công trình này thể hiện tiến trình hướng tới việc tạo ra các mô hình trao đổi chất dựa trên ràng buộc cụ thể đối với các điều kiện có sẵn dữ liệu lập hồ sơ biểu hiện."} +{"idx": 1960135, "en": "As modeling of changes in backbone conformation still lacks a computationally efficient solution, we developed a discretisation of the conformational states accessible to the protein backbone similar to the successful rotamer approach in side chains. The BriX fragment database, consisting of fragments from 4 to 14 residues long, was realized through identification of recurrent backbone fragments from a non-redundant set of high-resolution protein structures. BriX contains an alphabet of more than 1,000 frequently observed conformations per peptide length for 6 different variation levels. Analysis of the performance of BriX revealed an average structural coverage of protein structures of more than 99% within a root mean square distance (RMSD) of 1 Angstrom. Globally, we are able to reconstruct protein structures with an average accuracy of 0.48 Angstrom RMSD. As expected, regular structures are well covered, but, interestingly, many loop regions that appear irregular at first glance are also found to form a recurrent structural motif, albeit with lower frequency of occurrence than regular secondary structures. Larger loop regions could be completely reconstructed from smaller recurrent elements, between 4 and 8 residues long. Finally, we observed that a significant amount of short sequences tend to display strong structural ambiguity between alpha helix and extended conformations. When the sequence length increases, this so-called sequence plasticity is no longer observed, illustrating the context dependency of polypeptide structures.", "gemini": "Vì mô hình hóa những thay đổi trong cấu hình xương sống vẫn thiếu một giải pháp hiệu quả về mặt tính toán, chúng tôi đã phát triển một phương pháp rời rạc hóa các trạng thái cấu hình có thể tiếp cận được với xương sống protein tương tự như phương pháp rotamer thành công trong các chuỗi bên. Cơ sở dữ liệu đoạn BriX, bao gồm các đoạn từ 4 đến 14 phần còn lại dài, đã được thực hiện thông qua việc xác định các đoạn xương sống tái diễn từ một tập hợp không trùng lặp các cấu trúc protein có độ phân giải cao. BriX chứa một bảng chữ cái gồm hơn 1.000 cấu hình được quan sát thường xuyên trên mỗi độ dài peptide cho 6 mức độ biến thể khác nhau. Phân tích hiệu suất của BriX cho thấy độ phủ cấu trúc trung bình của các cấu trúc protein là hơn 99% trong khoảng cách bình phương trung bình căn bậc hai (RMSD) là 1 Angstrom. Nhìn chung, chúng tôi có thể tái tạo các cấu trúc protein với độ chính xác trung bình là 0,48 Angstrom RMSD. Như dự kiến, các cấu trúc thông thường được phủ tốt, nhưng điều thú vị là nhiều vùng vòng lặp có vẻ không đều ở cái nhìn đầu tiên cũng được tìm thấy tạo thành một họa tiết cấu trúc tái diễn, mặc dù tần suất xuất hiện thấp hơn các cấu trúc thứ cấp thông thường. Các vùng vòng lặp lớn hơn có thể được tái tạo hoàn toàn từ các phần tử tái diễn nhỏ hơn, dài từ 4 đến 8 phần còn lại. Cuối cùng, chúng tôi quan sát thấy rằng một lượng đáng kể các trình tự ngắn có xu hướng hiển thị sự mơ hồ về mặt cấu trúc mạnh mẽ giữa cấu hình xoắn alpha và cấu hình mở rộng. Khi độ dài trình tự tăng lên, cái gọi là tính dẻo của trình tự này không còn được quan sát thấy nữa, minh họa cho sự phụ thuộc vào ngữ cảnh của các cấu trúc polypeptide."} +{"idx": 1960136, "en": "In eutherian mammals, embryonic growth and survival is dependent on the formation of the placenta, an organ that facilitates the efficient exchange of oxygen, nutrients, and metabolic waste between the maternal and fetal blood supplies. Key to the placenta's function is the formation of its vascular labyrinth, a series of finely branched vessels whose molecular ontogeny remains largely undefined. In this report, we demonstrate that HOXA13 plays an essential role in labyrinth vessel formation. In the absence of HOXA13 function, placental endothelial cell morphology is altered, causing a loss in vessel wall integrity, edema of the embryonic blood vessels, and mid-gestational lethality. Microarray analysis of wild-type and mutant placentas revealed significant changes in endothelial gene expression profiles. Notably, pro-vascular genes, including Tie2 and Foxf1, exhibited reduced expression in the mutant endothelia, which also exhibited elevated expression of genes normally expressed in lymphatic or sinusoidal endothelia. ChIP analysis of HOXA13-DNA complexes in the placenta confirmed that HOXA13 binds the Tie2 and Foxf1 promoters in vivo. In vitro, HOXA13 binds sequences present in the Tie2 and Foxf1 promoters with high affinity (K(d) = 27-42 nM) and HOXA13 can use these bound promoter regions to direct gene expression. Taken together, these findings demonstrate that HOXA13 directly regulates Tie2 and Foxf1 in the placental labyrinth endothelia, providing a functional explanation for the mid-gestational lethality exhibited by Hoxa13 mutant embryos as well as a novel transcriptional program necessary for the specification of the labyrinth vascular endothelia.", "gemini": "Ở động vật có vú thuộc nhóm thú có nhau thai, sự phát triển và sự sống của phôi phụ thuộc vào sự hình thành của nhau thai, một cơ quan tạo điều kiện thuận lợi cho sự trao đổi hiệu quả oxy, chất dinh dưỡng và chất thải trao đổi chất giữa nguồn cung cấp máu của mẹ và thai nhi. Chìa khóa cho chức năng của nhau thai là sự hình thành của mê cung mạch máu, một loạt các mạch máu phân nhánh nhỏ mà quá trình hình thành phân tử vẫn chưa được xác định phần lớn. Trong báo cáo này, chúng tôi chứng minh rằng HOXA13 đóng vai trò thiết yếu trong quá trình hình thành mạch máu mê cung. Trong trường hợp không có chức năng HOXA13, hình thái tế bào nội mô nhau thai bị thay đổi, gây mất tính toàn vẹn của thành mạch, phù nề các mạch máu của phôi và tử vong giữa thời kỳ mang thai. Phân tích mảng gen của nhau thai kiểu hoang dã và đột biến cho thấy những thay đổi đáng kể trong các cấu hình biểu hiện gen nội mô. Đáng chú ý, các gen tiền mạch máu, bao gồm Tie2 và Foxf1, biểu hiện giảm ở nội mô đột biến, trong đó cũng biểu hiện cao các gen thường được biểu hiện ở nội mô bạch huyết hoặc xoang. Phân tích ChIP của các phức hợp HOXA13-DNA trong nhau thai xác nhận rằng HOXA13 liên kết với các vùng khởi động Tie2 và Foxf1 trong cơ thể sống. Trong ống nghiệm, HOXA13 liên kết với các trình tự có trong các vùng khởi động Tie2 và Foxf1 với ái lực cao (K(d) = 27-42 nM) và HOXA13 có thể sử dụng các vùng khởi động liên kết này để chỉ đạo biểu hiện gen. Tổng hợp lại, những phát hiện này chứng minh rằng HOXA13 điều chỉnh trực tiếp Tie2 và Foxf1 trong nội mô mê cung nhau thai, đưa ra lời giải thích về mặt chức năng cho sự tử vong giữa thai kỳ do phôi đột biến Hoxa13 biểu hiện cũng như một chương trình phiên mã mới cần thiết cho đặc điểm của nội mô mạch máu mê cung."} +{"idx": 1960137, "en": "In much of sub-Saharan Africa, the mosquito Anopheles gambiae is the main vector of the major human malaria parasite, Plasmodium falciparum. Convenient laboratory studies have identified mosquito genes that affect positively or negatively the developmental cycle of the model rodent parasite, P. berghei. Here, we use transcription profiling and reverse genetics to explore whether five disparate mosquito gene regulators of P. berghei development are also pertinent to A. gambiae/P. falciparum interactions in semi-natural conditions, using field isolates of this parasite and geographically related mosquitoes. We detected broadly similar albeit not identical transcriptional responses of these genes to the two parasite species. Gene silencing established that two genes affect similarly both parasites: infections are hindered by the intracellular local activator of actin cytoskeleton dynamics, WASP, but promoted by the hemolymph lipid transporter, ApoII/I. Since P. berghei is not a natural parasite of A. gambiae, these data suggest that the effects of these genes have not been drastically altered by constant interaction and co-evolution of A. gambiae and P. falciparum; this conclusion allowed us to investigate further the mode of action of these two genes in the laboratory model system using a suite of genetic tools and infection assays. We showed that both genes act at the level of midgut invasion during the parasite's developmental transition from ookinete to oocyst. ApoII/I also affects the early stages of oocyst development. These are the first mosquito genes whose significant effects on P. falciparum field isolates have been established by direct experimentation. Importantly, they validate for semi-field human malaria transmission the concept of parasite antagonists and agonists.", "gemini": "Ở phần lớn khu vực cận Sahara châu Phi, muỗi Anopheles gambiae là vật chủ chính của ký sinh trùng sốt rét ở người, Plasmodium falciparum. Các nghiên cứu trong phòng thí nghiệm thuận tiện đã xác định các gen muỗi ảnh hưởng tích cực hoặc tiêu cực đến chu kỳ phát triển của ký sinh trùng gặm nhấm mô hình, P. berghei. Ở đây, chúng tôi sử dụng hồ sơ phiên mã và di truyền học ngược để khám phá xem năm gen điều hòa gen muỗi khác nhau của sự phát triển của P. berghei cũng có liên quan đến tương tác A. gambiae/P. falciparum trong các điều kiện bán tự nhiên hay không, sử dụng các phân lập thực địa của ký sinh trùng này và các loài muỗi có liên quan về mặt địa lý. Chúng tôi phát hiện ra những phản ứng phiên mã tương tự nhau nhưng không giống hệt nhau của các gen này đối với hai loài ký sinh trùng. Việc làm gen im lặng đã xác định rằng hai gen ảnh hưởng tương tự đến cả hai ký sinh trùng: nhiễm trùng bị cản trở bởi chất hoạt hóa cục bộ trong tế bào của động lực cytoskeleton actin, WASP, nhưng được thúc đẩy bởi chất vận chuyển lipid huyết tương, ApoII/I. Vì P. berghei không phải là ký sinh trùng tự nhiên của A. gambiae, dữ liệu này cho thấy rằng tác dụng của các gen này không bị thay đổi đáng kể do sự tương tác liên tục và tiến hóa đồng thời của A. gambiae và P. falciparum; kết luận này cho phép chúng tôi điều tra thêm chế độ hoạt động của hai gen này trong hệ thống mô hình phòng thí nghiệm bằng cách sử dụng một bộ công cụ di truyền và xét nghiệm nhiễm trùng. Chúng tôi đã chỉ ra rằng cả hai gen đều hoạt động ở mức độ xâm nhập ruột giữa trong quá trình chuyển đổi phát triển của ký sinh trùng từ ookinete sang nang trứng. ApoII/I cũng ảnh hưởng đến các giai đoạn đầu của sự phát triển nang trứng. Đây là những gen muỗi đầu tiên có tác dụng đáng kể đối với các phân lập thực địa P. falciparum đã được xác định bằng cách thử nghiệm trực tiếp. Quan trọng hơn, chúng xác nhận khái niệm về chất đối kháng và chất chủ vận ký sinh trùng đối với quá trình lây truyền sốt rét ở người bán thực địa."} +{"idx": 1960138, "en": "Mutations in the PAX6 gene have been implicated in aniridia, a congenital malformation of the eye with severe hypoplasia of the iris. However, not all aniridia cases can be explained by mutations in the PAX6 gene. The purpose of this study was to enhance the molecular diagnosis of aniridia using multiplex ligation-dependent probe amplification (MLPA). Total genomic DNA was isolated from peripheral blood of 70 unrelated probands affected with aniridia. Polymerase chain reaction (PCR) was performed followed by automated bidirectional sequencing. Additionally, MLPA was performed. We identified 24 different point mutations in the PAX6 gene in 34 patients after sequencing. In eight additional patients, we identified a deletion of one or more exons of the PAX6 gene or in the 3' regulatory region of the PAX6 gene using MLPA. This work demonstrates the necessity to screen for larger deletions in the region of the PAX6 gene in addition to the sequencing of exons in the PAX6 gene. The mutation detection rate will increase from 49% to 60%. This shows that MLPA substantially enhances the molecular diagnosis of aniridia.", "gemini": "Các đột biến ở gen PAX6 đã được chứng minh là liên quan đến chứng aniridia, một dị tật bẩm sinh của mắt với tình trạng kém phát triển nghiêm trọng của mống mắt. Tuy nhiên, không phải tất cả các trường hợp aniridia đều có thể được giải thích bằng các đột biến ở gen PAX6. Mục đích của nghiên cứu này là tăng cường chẩn đoán phân tử về bệnh aniridia bằng cách sử dụng khuếch đại đầu dò phụ thuộc vào gắn kết đa điểm (MLPA). Tổng DNA của bộ gen được phân lập từ máu ngoại vi của 70 bệnh nhân không có quan hệ huyết thống bị ảnh hưởng bởi chứng aniridia. Phản ứng chuỗi polymerase (PCR) được thực hiện tiếp theo là giải trình tự hai chiều tự động. Ngoài ra, MLPA đã được thực hiện. Chúng tôi đã xác định được 24 đột biến điểm khác nhau ở gen PAX6 ở 34 bệnh nhân sau khi giải trình tự. Ở tám bệnh nhân khác, chúng tôi đã xác định được một đoạn xóa một hoặc nhiều exon của gen PAX6 hoặc trong vùng điều hòa 3' của gen PAX6 bằng cách sử dụng MLPA. Nghiên cứu này chứng minh sự cần thiết phải sàng lọc các đoạn xóa lớn hơn trong vùng gen PAX6 ngoài việc giải trình tự các exon trong gen PAX6. Tỷ lệ phát hiện đột biến sẽ tăng từ 49% lên 60%. Điều này cho thấy MLPA làm tăng đáng kể chẩn đoán phân tử về chứng aniridia."} +{"idx": 1960139, "en": "PURPOSE The aim of the study was to characterize the underlying mutation in a consanguineous family having cataracts. METHODS Family D having congenital cataracts was treated at the University Eye Clinics at Giessen (Germany). Lens material from surgeries was collected, immediately frozen at -80 degrees C, and used for cDNA production. Blood was taken from the proband and available family members. Polymerase chain reaction (PCR)-amplified DNA fragments were characterized by sequencing and restriction digestion. RESULTS The proband, AD, has a dense, triangular nuclear cataract. The parents are consanguineous, and the mother and grandmother suffer from a discrete, symmetric opacity of the fetal lens nucleus. The proband's lens cDNA showed a homozygous insertion of one G after position 776 of the GJA8 gene, leading to a frame shift and 123 novel amino acids. The homozygous mutation was confirmed in the genomic DNA and is also present in the cataract-operated brother of the proband; all other family members investigated were heterozygous. The mutation could not be detected in 96 healthy controls from Germany. CONCLUSIONS The ins776G mutation most likely causes a recessive triangular cataract with variable expressivity of a weak phenotype in heterozygotes.", "gemini": "MỤC ĐÍCH Mục đích của nghiên cứu là xác định đặc điểm đột biến tiềm ẩn trong một gia đình cận huyết có bệnh đục thủy tinh thể. PHƯƠNG PHÁP Gia đình D mắc bệnh đục thủy tinh thể bẩm sinh đã được điều trị tại Phòng khám mắt của Đại học Giessen (Đức). Vật liệu thủy tinh thể từ các ca phẫu thuật đã được thu thập, đông lạnh ngay ở nhiệt độ -80 độ C và được sử dụng để sản xuất cDNA. Máu được lấy từ bệnh nhân và các thành viên gia đình có sẵn. Các đoạn DNA được khuếch đại bằng phản ứng chuỗi polymerase (PCR) được đặc trưng bằng giải trình tự và tiêu hóa hạn chế. KẾT QUẢ Bệnh nhân AD có đục thủy tinh thể nhân hình tam giác dày đặc. Cha mẹ là cận huyết, mẹ và bà bị đục thủy tinh thể nhân hình tròn đối xứng. cDNA thủy tinh thể của bệnh nhân cho thấy một đoạn chèn đồng hợp tử một G sau vị trí 776 của gen GJA8, dẫn đến dịch khung đọc và 123 axit amin mới. Đột biến đồng hợp tử đã được xác nhận trong DNA của bộ gen và cũng có mặt ở anh trai bị đục thủy tinh thể đã được phẫu thuật của bệnh nhân; tất cả các thành viên gia đình khác được điều tra đều là dị hợp tử. Đột biến không thể phát hiện thấy ở 96 người đối chứng khỏe mạnh từ Đức. KẾT LUẬN Đột biến ins776G rất có thể gây ra bệnh đục thủy tinh thể tam giác lặn với mức độ biểu hiện khác nhau của kiểu hình yếu ở những người dị hợp tử."} +{"idx": 1960140, "en": "PURPOSE Exfoliation syndrome (XFS) is characterized by an accumulation of abnormal extracellular material in the anterior part of the eye that frequently leads to increased intraocular pressure and glaucomatous optic neuropathy. Recently, two non-synonymous polymorphisms (rs1048661 G>T and rs3825942 G>A) of lysyl oxidase-like protein 1 (LOXL1), a monoamine oxidase that catalyzes the polymerization of tropoelastin to elastin, were found to be associated with increased risk for XFS and exfoliation glaucoma (XFG). The aim of the present study was to investigate the role of these LOXL1 variants in a Central European cohort of Caucasian patients with XFG. METHODS The present case-control study comprised of 167 unrelated patients with XFG and 170 control subjects. Genotyping of the LOXL1 rs1048661 and rs3825942 polymorphisms was done using polymerase chain reaction. RESULTS The frequency of allele G of rs1048661 as well as rs3825942 was significantly higher in patients than in controls (rs1048661: 0.841 in patients versus 0.669; p<0.001; rs3825942: 0.994 in patients versus 0.817; p<0.001). Odds ratios of 52.1 (95% confidence interval [CI]: 13.85-195.6) and 14.67 (95% CI: 3.81-56.2), respectively, were calculated for the two high-risk haplotypes GG and TG compared to the haplotype GA. CONCLUSIONS Our data confirm the previously reported association between LOXL1 polymorphisms and XFG and extend our knowledge to a Central European population.", "gemini": "MỤC ĐÍCH Hội chứng bong vảy (XFS) được đặc trưng bởi sự tích tụ vật liệu ngoại bào bất thường ở phần trước của mắt, thường dẫn đến tăng nhãn áp và bệnh lý thần kinh thị giác tăng nhãn áp. Gần đây, hai đa hình không đồng nghĩa (rs1048661 G>T và rs3825942 G>A) của protein giống lysyl oxidase-1 (LOXL1), một monoamine oxidase xúc tác quá trình trùng hợp của tropoelastin thành elastin, được phát hiện có liên quan đến nguy cơ mắc XFS và tăng nhãn áp bong vảy (XFG). Mục đích của nghiên cứu hiện tại là điều tra vai trò của các biến thể LOXL1 này trong một nhóm bệnh nhân người da trắng ở Trung Âu mắc XFG. PHƯƠNG PHÁP Nghiên cứu ca đối chứng hiện tại bao gồm 167 bệnh nhân không có quan hệ huyết thống mắc XFG và 170 đối tượng đối chứng. Kiểu gen của các đa hình LOXL1 rs1048661 và rs3825942 được thực hiện bằng phản ứng chuỗi polymerase. KẾT QUẢ Tần số alen G của rs1048661 cũng như rs3825942 cao hơn đáng kể ở bệnh nhân so với nhóm đối chứng (rs1048661: 0,841 ở bệnh nhân so với 0,669; p < 0,001; rs3825942: 0,994 ở bệnh nhân so với 0,817; p < 0,001). Tỷ lệ tỷ số chênh lệch là 52,1 (khoảng tin cậy 95% [CI]: 13,85-195,6) và 14,67 (95% CI: 3,81-56,2), tương ứng, được tính cho hai kiểu gen rủi ro cao GG và TG so với kiểu gen GA. KẾT LUẬN Dữ liệu của chúng tôi xác nhận mối liên quan đã được báo cáo trước đây giữa các đa hình LOXL1 và XFG và mở rộng kiến thức của chúng tôi cho một quần thể Trung Âu."} +{"idx": 1960141, "en": "Sorghum (Sorghum bicolor (L.) Moench) is a subject of plant genomics research based on its importance as one of the world's leading cereal crops, a biofuels crop of high and growing importance, a progenitor of one of the world's most noxious weeds, and a botanical model for many tropical grasses with complex genomes. A rich history of genome analysis, culminating in the recent complete sequencing of the genome of a leading inbred, provides a foundation for invigorating progress toward relating sorghum genes to their functions. Further characterization of the genomes other than Saccharinae cereals may shed light on mechanisms, levels, and patterns of evolution of genome size and structure, laying the foundation for further study of sugarcane and other economically important members of the group.", "gemini": "Lúa miến (Sorghum bicolor (L.) Moench) là đối tượng nghiên cứu về di truyền học thực vật dựa trên tầm quan trọng của nó như một trong những loại cây lương thực hàng đầu thế giới, một loại cây trồng làm nhiên liệu sinh học có tầm quan trọng cao và ngày càng tăng, là tổ tiên của một trong những loại cỏ dại nguy hiểm nhất thế giới và là mô hình thực vật cho nhiều loại cỏ nhiệt đới có bộ gen phức tạp. Lịch sử phân tích bộ gen phong phú, đỉnh cao là quá trình giải trình tự hoàn chỉnh gần đây của bộ gen của một giống thuần chủng hàng đầu, cung cấp nền tảng để thúc đẩy tiến trình liên quan đến gen lúa miến với chức năng của chúng. Việc mô tả đặc điểm thêm về các bộ gen khác ngoài các loại ngũ cốc Saccharinae có thể làm sáng tỏ các cơ chế, mức độ và kiểu tiến hóa của kích thước và cấu trúc bộ gen, đặt nền tảng cho việc nghiên cứu sâu hơn về mía và các thành viên quan trọng khác về mặt kinh tế của nhóm."} +{"idx": 1960142, "en": "Although N-acetylcysteine (NAC) has been shown to be neuroprotective for traumatic brain injury (TBI), the mechanisms for this beneficial effect are still poorly understood. Cerebral inflammation plays an important role in the pathogenesis of secondary brain injury after TBI. However, it has not been investigated whether NAC modulates TBI-induced cerebral inflammatory response. In this work, we investigated the effect of NAC administration on cortical expressions of nuclear factor kappa B (NF-kappaB) and inflammatory proteins such as interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and intercellular adhesion molecule-1 (ICAM-1) after TBI. As a result, we found that NF-kappaB, proinflammatory cytokines, and ICAM-1 were increased in all injured animals. In animals given NAC post-TBI, NF-kappaB, IL-1beta, TNF-alpha, and ICAM-1 were decreased in comparison to vehicle-treated animals. Measures of IL-6 showed no change after NAC treatment. NAC administration reduced brain edema, BBB permeability, and apoptotic index in the injured brain. The results suggest that post-TBI NAC administration may attenuate inflammatory response in the injured rat brain, and this may be one mechanism by which NAC ameliorates secondary brain damage following TBI.", "gemini": "Mặc dù N-acetylcysteine (NAC) đã được chứng minh là có tác dụng bảo vệ thần kinh đối với chấn thương sọ não (TBI), nhưng cơ chế gây ra tác dụng có lợi này vẫn chưa được hiểu rõ. Viêm não đóng vai trò quan trọng trong quá trình sinh bệnh của chấn thương não thứ phát sau TBI. Tuy nhiên, vẫn chưa có nghiên cứu nào tìm hiểu xem NAC có điều chỉnh phản ứng viêm não do TBI gây ra hay không. Trong nghiên cứu này, chúng tôi đã điều tra tác dụng của việc dùng NAC lên biểu hiện của yếu tố hạt nhân kappa B (NF-kappaB) ở vỏ não và các protein gây viêm như interleukin-1beta (IL-1beta), yếu tố hoại tử khối u-alpha (TNF-alpha), interleukin-6 (IL-6) và phân tử bám dính giữa các tế bào-1 (ICAM-1) sau TBI. Kết quả là, chúng tôi thấy rằng NF-kappaB, cytokine tiền viêm và ICAM-1 tăng ở tất cả các động vật bị thương. Ở những động vật được dùng NAC sau TBI, NF-kappaB, IL-1beta, TNF-alpha và ICAM-1 giảm so với những động vật được điều trị bằng dung môi. Các biện pháp của IL-6 không thay đổi sau khi điều trị bằng NAC. Việc dùng NAC làm giảm phù não, tính thấm BBB và chỉ số tế bào chết theo chương trình ở não bị thương. Kết quả cho thấy rằng việc dùng NAC sau TBI có thể làm giảm phản ứng viêm ở não chuột bị thương và đây có thể là một cơ chế mà NAC cải thiện tổn thương não thứ phát sau TBI."} +{"idx": 1960143, "en": "There has been much interest in CpG islands (CGIs), clusters of CpG dinucleotides in GC-rich regions, because they are considered gene markers and involved in gene regulation. To date, there has been no genome-wide analysis of CGIs in the fish genome. We first evaluated the performance of three popular CGI identification algorithms in four fish genomes (tetraodon, stickleback, medaka, and zebrafish). Our results suggest that Takai and Jones' (2002) algorithm is most suitable for comparative analysis of CGIs in the fish genome. Then, we performed a systematic analysis of CGIs in the four fish genomes using Takai and Jones' algorithm, compared to other vertebrate genomes. We found that both the number of CGIs and the CGI density vary greatly among these genomes. Remarkably, each fish genome presents a distinct distribution of CGI density with some genomic factors (e.g., chromosome size and chromosome GC content). These findings are helpful for understanding evolution of fish genomes and the features of fish CGIs.", "gemini": "Có rất nhiều sự quan tâm đến các đảo CpG (CGI), các cụm dinucleotide CpG trong các vùng giàu GC, vì chúng được coi là dấu hiệu gen và có liên quan đến điều hòa gen. Cho đến nay, vẫn chưa có phân tích nào trên toàn bộ hệ gen về CGI trong hệ gen cá. Đầu tiên, chúng tôi đã đánh giá hiệu suất của ba thuật toán xác định CGI phổ biến trong bốn bộ gen cá (cá nóc, cá bống gai, cá medaka và cá ngựa vằn). Kết quả của chúng tôi cho thấy rằng thuật toán của Takai và Jones (2002) phù hợp nhất để phân tích so sánh CGI trong bộ gen cá. Sau đó, chúng tôi đã thực hiện phân tích hệ thống CGI trong bốn bộ gen cá bằng thuật toán của Takai và Jones, so sánh với các bộ gen của động vật có xương sống khác. Chúng tôi thấy rằng cả số lượng CGI và mật độ CGI đều thay đổi rất nhiều giữa các bộ gen này. Đáng chú ý, mỗi bộ gen cá đều thể hiện sự phân bố mật độ CGI riêng biệt với một số yếu tố về bộ gen (ví dụ: kích thước nhiễm sắc thể và hàm lượng GC của nhiễm sắc thể). Những phát hiện này rất hữu ích để hiểu về sự tiến hóa của bộ gen cá và các đặc điểm của CGI ở cá."} diff --git a/retranslated_vi_pubmed/chunk_196/error.json b/retranslated_vi_pubmed/chunk_196/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_196/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_197/completed.json b/retranslated_vi_pubmed/chunk_197/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..590d381748ae69c8bd0af5aef617d96fa9211fe8 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_197/completed.json @@ -0,0 +1,146 @@ +[ + 1970000, + 1970001, + 1970002, + 1970003, + 1970004, + 1970005, + 1970006, + 1970007, + 1970008, + 1970009, + 1970010, + 1970011, + 1970012, + 1970013, + 1970014, + 1970015, + 1970016, + 1970017, + 1970018, + 1970019, + 1970020, + 1970021, + 1970022, + 1970023, + 1970024, + 1970025, + 1970026, + 1970027, + 1970028, + 1970029, + 1970030, + 1970031, + 1970032, + 1970033, + 1970034, + 1970035, + 1970036, + 1970037, + 1970038, + 1970039, + 1970040, + 1970041, + 1970042, + 1970043, + 1970044, + 1970045, + 1970046, + 1970047, + 1970048, + 1970049, + 1970050, + 1970051, + 1970052, + 1970053, + 1970054, + 1970055, + 1970056, + 1970057, + 1970058, + 1970059, + 1970060, + 1970061, + 1970062, + 1970063, + 1970064, + 1970065, + 1970066, + 1970067, + 1970068, + 1970069, + 1970070, + 1970071, + 1970072, + 1970073, + 1970074, + 1970075, + 1970076, + 1970077, + 1970078, + 1970079, + 1970080, + 1970081, + 1970082, + 1970083, + 1970084, + 1970085, + 1970086, + 1970087, + 1970088, + 1970089, + 1970090, + 1970091, + 1970092, + 1970093, + 1970094, + 1970095, + 1970096, + 1970097, + 1970098, + 1970099, + 1970100, + 1970101, + 1970102, + 1970103, + 1970104, + 1970105, + 1970106, + 1970107, + 1970108, + 1970109, + 1970110, + 1970111, + 1970112, + 1970113, + 1970114, + 1970115, + 1970116, + 1970117, + 1970118, + 1970119, + 1970120, + 1970121, + 1970122, + 1970123, + 1970124, + 1970125, + 1970126, + 1970127, + 1970128, + 1970129, + 1970130, + 1970131, + 1970132, + 1970133, + 1970134, + 1970135, + 1970136, + 1970137, + 1970138, + 1970139, + 1970140, + 1970141, + 1970142, + 1970143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_197/data.jsonl b/retranslated_vi_pubmed/chunk_197/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..1a1c98b39631c3a0f44be506346e7edb4d5a88fc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_197/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1970000, "en": "A 3 year study of the infiltration of Lot River water into a well field located in an adjacent gravel and clay alluvial aquifer was conducted to assess the importance of organic matter regarding the redox processes which influence groundwater quality in a positive (denitrification) or negative (Mn dissolution) manner. Chloride was used to quantify the mixing of river water with groundwater. According to modeling with PHREEQC, the biodegradation of the infiltrated dissolved organic carbon (DOCi) is not sufficient to explain the observed consequences of the redox reactions (dissolved O2 depletion, denitrification, Mn dissolution). Another electron donor source must therefore be involved: we propose solid organic carbon (SOC) as a likely candidate, if made available for degradation by prior hydrolysis. Its contribution to redox reactions could be significant (30-80% of the total organic carbon consumed by redox reactions during river bank filtration). We show here also that even though the first few meters of infiltration are highly reactive, significant redox reactions can take place further in the aquifer, possibly affecting groundwater quality away from the river bank.", "gemini": "Một nghiên cứu trong 3 năm về sự xâm nhập của nước sông Lot vào một vùng giếng nằm trong một tầng chứa nước phù sa sỏi và đất sét liền kề đã được tiến hành để đánh giá tầm quan trọng của chất hữu cơ liên quan đến các quá trình oxy hóa khử ảnh hưởng đến chất lượng nước ngầm theo hướng tích cực (khử nitrat) hoặc tiêu cực (hòa tan Mn). Clorua được sử dụng để định lượng sự trộn lẫn của nước sông với nước ngầm. Theo mô hình hóa với PHREEQC, sự phân hủy sinh học của carbon hữu cơ hòa tan xâm nhập (DOCi) là không đủ để giải thích hậu quả quan sát được của phản ứng oxy hóa khử (hết O2 hòa tan, khử nitrat, hòa tan Mn). Do đó, phải có một nguồn cung cấp điện tử khác: chúng tôi đề xuất carbon hữu cơ rắn (SOC) là một ứng cử viên có khả năng, nếu được phân hủy bằng thủy phân trước đó. Sự đóng góp của nó vào các phản ứng oxy hóa khử có thể rất đáng kể (30-80% tổng lượng carbon hữu cơ được tiêu thụ bởi các phản ứng oxy hóa khử trong quá trình lọc bờ sông). Chúng tôi cũng chỉ ra ở đây rằng mặc dù vài mét đầu tiên của sự xâm nhập có phản ứng cao, nhưng các phản ứng oxy hóa khử đáng kể có thể diễn ra xa hơn trong tầng chứa nước, có thể ảnh hưởng đến chất lượng nước ngầm cách xa bờ sông."} +{"idx": 1970001, "en": "Bacterial spore determination by terbium(III)-dipicolinate luminescence has been reported by several investigators. We collected spore samples with a cyclone and extracted dipicolinic acid (DPA) in-line with hot aqueous dodecylamine, added Tb(III) in a continuous-flow system and detected the Tb(III)-DPA with a gated liquid core waveguide fluorescence detector with a flashlamp excitation source. The absolute limit of detection (LOD) for the system was equivalent to 540 B. subtilis spores (for a 1.8 m3 sample volume (t = 2 h, Q = 15 L/min), concentration LOD is 0.3 spores/L air). Extant literature suggests that, from office to home settings, viable spore concentrations range from 0.1 to 10 spores/L; however, these data have never been validated. Previously reported semiautomated instrumentation had an LOD of 50 spores/L. The present system was tested at five different location settings in Lubbock, Texas. The apparent bacterial spore concentrations ranged from 9 to 700 spores/L and only occasionally exhibited the same trend as the simultaneously monitored total optical particle counts in the > or = 0.5 microm size fraction. However, because the apparent spore counts sometimes were very large relative to the 0.5+ microm size particle counts, we investigated potential positive interferences. We show that aromatic acids are very likely large interferents. This interference typically constitutes approximately 70% of the signal and can be as high as 95%. It can be completely removed by prewashing the particles.", "gemini": "Một số nhà nghiên cứu đã báo cáo về việc xác định bào tử vi khuẩn bằng phát quang terbium(III)-dipicolinate. Chúng tôi đã thu thập các mẫu bào tử bằng lốc xoáy và chiết xuất axit dipicolinic (DPA) trực tuyến với dodecylamine nước nóng, thêm Tb(III) vào hệ thống dòng chảy liên tục và phát hiện Tb(III)-DPA bằng máy dò huỳnh quang lõi chất lỏng có cổng với nguồn kích thích đèn flash. Giới hạn phát hiện tuyệt đối (LOD) cho hệ thống tương đương với 540 bào tử B. subtilis (đối với thể tích mẫu 1,8 m3 (t = 2 h, Q = 15 L/phút), LOD nồng độ là 0,3 bào tử/L không khí). Các tài liệu hiện có cho thấy, từ văn phòng đến nhà, nồng độ bào tử khả thi dao động từ 0,1 đến 10 bào tử/L; tuy nhiên, những dữ liệu này chưa bao giờ được xác thực. Các thiết bị bán tự động được báo cáo trước đây có LOD là 50 bào tử/L. Hệ thống hiện tại đã được thử nghiệm tại năm địa điểm khác nhau ở Lubbock, Texas. Nồng độ bào tử vi khuẩn biểu kiến dao động từ 9 đến 700 bào tử/L và chỉ thỉnh thoảng biểu hiện cùng xu hướng với số lượng hạt quang học tổng số được theo dõi đồng thời trong phân đoạn kích thước lớn hơn hoặc bằng 0,5 microm. Tuy nhiên, vì số lượng bào tử biểu kiến đôi khi rất lớn so với số lượng hạt kích thước 0,5+ microm, chúng tôi đã điều tra các nhiễu tích cực tiềm ẩn. Chúng tôi chỉ ra rằng các axit thơm rất có thể là chất gây nhiễu lớn. Sự nhiễu này thường chiếm khoảng 70% tín hiệu và có thể lên tới 95%. Nó có thể được loại bỏ hoàn toàn bằng cách rửa sạch các hạt trước."} +{"idx": 1970002, "en": "Three high volume septic systems in Ontario, Canada, were examined to assess the potential for onsite wastewatertreatment systems to release pharmaceutical compounds to the environment and to evaluate the mobility of these compounds in receiving aquifers. Wastewater samples were collected from the septic tanks, and groundwater samples were collected below and down gradient of the infiltration beds and analyzed for a suite of commonly used pharmaceutical and trace organic compounds. The septic tank samples contained elevated concentrations of several pharmaceutical compounds. Ten of the 12 compounds analyzed were detected in groundwater at one or more sites at concentrations in the low ng L(-1) to low microg L(-1) range. Large differences among the sites were observed in both the number of detections and the concentrations of the pharmaceutical compounds. Of the compounds analyzed, ibuprofen, gemfibrozil, and naproxen were observed to be transported atthe highest concentrations and greatest distances from the infiltration source areas, particularly in anoxic zones of the plumes.", "gemini": "Ba hệ thống tự hoại khối lượng lớn ở Ontario, Canada, đã được kiểm tra để đánh giá tiềm năng của các hệ thống xử lý nước thải tại chỗ trong việc thải các hợp chất dược phẩm ra môi trường và đánh giá khả năng di động của các hợp chất này trong các tầng chứa nước tiếp nhận. Các mẫu nước thải được thu thập từ các bể tự hoại và các mẫu nước ngầm được thu thập bên dưới và theo hướng dốc của các luống thấm và được phân tích để tìm ra một loạt các hợp chất hữu cơ vi lượng và dược phẩm được sử dụng phổ biến. Các mẫu bể tự hoại có chứa nồng độ cao của một số hợp chất dược phẩm. Mười trong số 12 hợp chất được phân tích đã được phát hiện trong nước ngầm tại một hoặc nhiều địa điểm với nồng độ trong phạm vi ng L(-1) thấp đến microg L(-1) thấp. Sự khác biệt lớn giữa các địa điểm được quan sát thấy ở cả số lần phát hiện và nồng độ của các hợp chất dược phẩm. Trong số các hợp chất được phân tích, ibuprofen, gemfibrozil và naproxen được quan sát là được vận chuyển ở nồng độ cao nhất và khoảng cách xa nhất từ các khu vực nguồn thấm, đặc biệt là ở các vùng thiếu oxy của các đám mây."} +{"idx": 1970003, "en": "In this study we have evaluated the role of wildfires on concentrations of fine particle (d < 2.5 microm) organic carbon (OC) and particulate mass (PM2.5) in the Western United States for the period 1988-2004. To do this, we examined the relationship between mean summer PM2.5 and OC concentrations at 39 IMPROVE sites with a database of fires developed from federal fire reports. The gridded database of area burned was used to generate a database of biomass fuel burned using ecosystem-specific fuel loads. The OC, PM2.5, and fire data were evaluated for five regions: Northern Rocky Mountains (Region 1), Central Rocky Mountains (Region 2), Southwest (Region 3), California (Region 4), and Pacific Northwest (Region 5). In Regions 1, 2, and 5, we found good correlations of seasonal mean PM2.5 concentrations among the sites within each region. This indicates that a common influence was important in determining the PM concentration at all sites across each region. In Regions 1 and 2, we found a significant correlation between PM2.5 and both the area burned and biomassfuel burned in each region. This relationship is statistically significant using either the area burned or fuel burned, but the correlations are stronger using the biomass fuel burned. In all five regions we found a statistically significant relationship between biomass burned and organic carbon. Using these relationships, we can estimate the amount of PM2.5 due to fires in each region during summer. For the Regions 1 through 5, the average summer-long enhancement of PM2.5 due to fires is 1.84, 1.09, 0.61, 0.81, and 1.21 microg/m3, respectively, and approximately twice these values during large fire years.", "gemini": "Trong nghiên cứu này, chúng tôi đã đánh giá vai trò của cháy rừng đối với nồng độ hạt mịn (d < 2,5 microm) carbon hữu cơ (OC) và khối lượng hạt (PM2.5) ở miền Tây Hoa Kỳ trong giai đoạn 1988-2004. Để thực hiện việc này, chúng tôi đã kiểm tra mối quan hệ giữa nồng độ PM2.5 và OC trung bình vào mùa hè tại 39 địa điểm của IMPROVE với cơ sở dữ liệu về các vụ cháy được phát triển từ các báo cáo hỏa hoạn của liên bang. Cơ sở dữ liệu lưới về diện tích bị cháy đã được sử dụng để tạo ra cơ sở dữ liệu về nhiên liệu sinh khối bị đốt cháy bằng cách sử dụng tải trọng nhiên liệu riêng cho từng hệ sinh thái. Dữ liệu về OC, PM2.5 và hỏa hoạn đã được đánh giá cho năm khu vực: Dãy núi Rocky phía Bắc (Khu vực 1), Dãy núi Rocky trung tâm (Khu vực 2), Tây Nam (Khu vực 3), California (Khu vực 4) và Tây Bắc Thái Bình Dương (Khu vực 5). Ở các Khu vực 1, 2 và 5, chúng tôi thấy có mối tương quan tốt về nồng độ PM2.5 trung bình theo mùa giữa các địa điểm trong mỗi khu vực. Điều này chỉ ra rằng một tác động chung rất quan trọng trong việc xác định nồng độ PM tại tất cả các địa điểm trên mỗi khu vực. Ở các Khu vực 1 và 2, chúng tôi thấy có mối tương quan đáng kể giữa PM2.5 và cả diện tích bị cháy và nhiên liệu sinh khối bị cháy ở mỗi khu vực. Mối quan hệ này có ý nghĩa thống kê khi sử dụng diện tích bị cháy hoặc nhiên liệu bị cháy, nhưng mối tương quan mạnh hơn khi sử dụng nhiên liệu sinh khối bị cháy. Ở cả năm khu vực, chúng tôi thấy có mối quan hệ có ý nghĩa thống kê giữa sinh khối bị cháy và carbon hữu cơ. Sử dụng các mối quan hệ này, chúng ta có thể ước tính lượng PM2.5 do cháy rừng ở mỗi khu vực trong mùa hè. Đối với các Khu vực từ 1 đến 5, sự gia tăng PM2.5 trung bình trong suốt mùa hè do cháy rừng lần lượt là 1,84, 1,09, 0,61, 0,81 và 1,21 microg/m3 và gấp khoảng hai lần các giá trị này trong những năm có cháy rừng lớn."} +{"idx": 1970004, "en": "The effect of soil organic status on copper impact was investigated by means of a microcosm study carried out on a vineyard soil that had been amended with varying types of organic matter during a previous long-term field experiment. Soil microcosms were contaminated at 250 mg Cu kg(-1) and incubated for 35 days. Copper distribution and dynamics were assessed in the solid matrix by a sequential extraction procedure and in the soil solution by measuring total and free exchangeable copper concentrations. Copper bioavailability was also measured with a whole-cell biosensor. Modifications of microbial communities were assessed by means of biomass-C measurements and characterization of genetic structure using ARISA (automated-ribosomal-intergenic-spacer-analysis). The results showed that copper distribution, speciation, and bioavailability are strongly different between organically amended and nonamended soils. Surprisingly, in solution, bioavailable copper correlated with total copper but not with free copper. Similarly the observed differential copper impact on micro-organisms suggested that organic matter controlled copper toxicity. Bacterial-ARISA modifications also correlated with the estimated metal bioavailability and corresponded to the enrichment of the Actinobacteria. Contrarily, biomass-C and fungal-ARISA measurements did not relate trivially to copper speciation and bioavailability, suggesting that the specific composition of the indigenous-soil communities controls its sensitivity to this metal.", "gemini": "Tác động của tình trạng hữu cơ của đất đối với tác động của đồng đã được nghiên cứu bằng một nghiên cứu vi mô được thực hiện trên đất vườn nho đã được bổ sung các loại chất hữu cơ khác nhau trong một thí nghiệm thực địa dài hạn trước đó. Các vi mô đất bị ô nhiễm ở mức 250 mg Cu kg(-1) và được ủ trong 35 ngày. Phân phối và động lực của đồng được đánh giá trong ma trận rắn bằng quy trình chiết tuần tự và trong dung dịch đất bằng cách đo nồng độ đồng trao đổi tự do và tổng số. Khả dụng sinh học của đồng cũng được đo bằng cảm biến sinh học toàn tế bào. Những thay đổi của quần xã vi khuẩn được đánh giá bằng cách đo sinh khối-C và đặc tính cấu trúc di truyền bằng cách sử dụng ARISA (phân tích khoảng cách gen giữa riboxome tự động). Kết quả cho thấy sự phân bố, hình thái và khả dụng sinh học của đồng rất khác nhau giữa đất được bổ sung hữu cơ và đất không được bổ sung hữu cơ. Đáng ngạc nhiên là trong dung dịch, đồng có khả dụng sinh học tương quan với tổng lượng đồng nhưng không phải với đồng tự do. Tương tự như vậy, tác động khác biệt của đồng đối với vi sinh vật cho thấy rằng chất hữu cơ kiểm soát độc tính của đồng. Các sửa đổi ARISA của vi khuẩn cũng tương quan với khả dụng sinh học của kim loại ước tính và tương ứng với sự làm giàu của Actinobacteria. Ngược lại, các phép đo sinh khối-C và ARISA của nấm không liên quan tầm thường đến hình thái và khả dụng sinh học của đồng, điều này cho thấy rằng thành phần cụ thể của các quần xã đất bản địa kiểm soát độ nhạy của nó đối với kim loại này."} +{"idx": 1970005, "en": "Deposition behaviors of carboxylate-modified polystyrene latex microspheres (five sizes ranging from 0.1 to 2.0 microm) were examined in packed porous media, impinging jet, and porous media-packed flow chamber systems under a variety of environmentally relevant ionic strength and flow conditions in the presence of an energy barrier to deposition. Temporally constant deposition rate coefficients were observed for all microsphere sizes under baseline conditions, whereas temporal increases in colloid deposition rate coefficients (ripening) occurred for all microsphere sizes in response to slight increases in solution ionic strength and slight decreases in fluid velocity. This transition from \"clean bed\" deposition to ripening was triggered by relatively subtle changes in solution chemistry and fluid velocity. Direct observation of colloid deposition in a flow chamber packed with porous media revealed that colloidal aggregates formed at grain-to-grain contacts in the porous media. The absence of ripening in an impinging jet system (unbounded flat surface) examined under equivalent conditions to the packed porous media further indicated that colloid aggregation was driven by the funneling of fluid into the grain-to-grain contacts. Comparison of colloid breakthrough in porous media comprised of smooth-spherical versus angular grains demonstrated that the propensity to trigger ripening increased with the number and length of grain-to-grain contacts.", "gemini": "Hành vi bám dính của các vi cầu latex polystyrene biến tính bằng cacboxylat (năm kích thước từ 0,1 đến 2,0 microm) đã được kiểm tra trong các hệ thống phương tiện xốp đóng gói, tia phun va chạm và buồng dòng chảy chứa phương tiện xốp đóng gói trong nhiều điều kiện dòng chảy và cường độ ion có liên quan đến môi trường khi có rào cản năng lượng đối với sự bám dính. Hệ số tốc độ bám dính không đổi theo thời gian đã được quan sát thấy đối với tất cả các kích thước vi cầu trong các điều kiện cơ bản, trong khi sự gia tăng theo thời gian về hệ số tốc độ bám dính của keo (chín) xảy ra đối với tất cả các kích thước vi cầu để đáp ứng với sự gia tăng nhẹ về cường độ ion của dung dịch và sự giảm nhẹ về vận tốc chất lỏng. Sự chuyển đổi này từ sự bám dính của \"lớp phủ sạch\" sang chín được kích hoạt bởi những thay đổi tương đối tinh tế trong hóa học của dung dịch và vận tốc chất lỏng. Quan sát trực tiếp sự bám dính của keo trong buồng dòng chảy chứa phương tiện xốp đóng gói cho thấy rằng các tập hợp keo hình thành tại các tiếp điểm hạt-hạt trong phương tiện xốp. Việc không chín trong hệ thống tia phun va chạm (bề mặt phẳng không giới hạn) được kiểm tra trong các điều kiện tương đương với phương tiện xốp đóng gói càng chỉ ra rằng sự kết tụ keo được thúc đẩy bởi sự dẫn hướng chất lỏng vào các tiếp điểm hạt-hạt. So sánh sự đột phá của keo trong phương tiện xốp bao gồm các hạt hình cầu mịn so với các hạt góc cạnh cho thấy rằng khả năng gây chín tăng lên theo số lượng và chiều dài của các tiếp điểm hạt-hạt."} +{"idx": 1970006, "en": "Copper retention by ferrihydrite, leaf compost, and montmorillonite was studied over 8 months in systems that emulate a natural soil where different solid phases compete for Cu through a common solution in a compartmentalized batch reactor. Copper speciation in solution (total dissolved, DPASV-labile, and free) and exchangeable and total Cu in individual solid phases were determined. Organic carbon in solution (DOC) and that retained by the mineral phases were also determined. Cu sorption reached steady-state after 4 months and accounted for 80% of the Cu initially added to the system (0.15 mg L(-1)). The remaining 20% stayed in solution as nonlabile (82.8%), labile (17%), and free (0.2%) Cu species. Copper sorption followed the order organic matter > silicate clays > iron oxides. Within each solid phase, exchangeable Cu was < or = 10% of the total Cu sorbed. DOC reached steady state (22 mg L(-1)) after 4 months and seemed to control Cu solubility and sorption behavior by the formation of soluble Cu-DOC complexes and by sorbing onto the mineral phases. DOC sorption onto ferrihydrite prevented Cu retention by this solid phase. Using a multicomponent system and 8 months equilibrations, we were able to capture some of the more important aspects of the complexity of soil environments bytaking into account diffusion processes and competition among solid- and solution-phase soil constituents in the retention of a metal cation.", "gemini": "Sự giữ đồng của ferrihydrit, phân trộn lá và montmorillonit đã được nghiên cứu trong 8 tháng trong các hệ thống mô phỏng đất tự nhiên, trong đó các pha rắn khác nhau cạnh tranh Cu thông qua một dung dịch chung trong lò phản ứng mẻ được ngăn thành từng ngăn. Các dạng đồng trong dung dịch (tổng số hòa tan, DPASV-có thể phân hủy và tự do) và đồng có thể trao đổi và tổng số đồng trong từng pha rắn đã được xác định. Cacbon hữu cơ trong dung dịch (DOC) và lượng cacbon hữu cơ được giữ lại bởi các pha khoáng cũng được xác định. Sự hấp phụ Cu đạt trạng thái ổn định sau 4 tháng và chiếm 80% lượng Cu ban đầu được thêm vào hệ thống (0,15 mg L(-1)). 20% còn lại tồn tại trong dung dịch dưới dạng các loài Cu không ổn định (82,8%), không ổn định (17%) và tự do (0,2%). Sự hấp phụ đồng tuân theo thứ tự vật chất hữu cơ> sét silicat> oxit sắt. Trong mỗi pha rắn, đồng có thể trao đổi or = 30 mM, 80% of the U was reduced to U(IV), with transient reoxidation at about 150 days. Mn(III,IV) oxides were completely reduced to Mn(II) in sediments infused with OC > or = 3 mM. However, Fe remained largely unreduced in all sediment columns, showing that Fe(III) can persist as an electron acceptor in reducing sediments over long times. This result in combination with the complete reduction of all other potential electron acceptors supports the hypothesis that the reactive Fe(III) fraction was responsible for reoxidizing U(IV).", "gemini": "Trạng thái oxy hóa của urani trong trầm tích rất quan trọng vì độ hòa tan của nguyên tố độc hại và phóng xạ này đối với loài U(VI) lớn hơn nhiều so với loài U(IV). Do đó, việc thao tác khử oxy hóa để thúc đẩy quá trình kết tủa UO2 đang được quan tâm như một phương pháp phục hồi trầm tích bị nhiễm U. Sự có mặt của oxit Fe và Mn trong trầm tích ở nồng độ cao hơn nhiều so với U cũng đòi hỏi phải hiểu về trạng thái khử oxy hóa của chúng. Nghiên cứu này được tiến hành để xác định những thay đổi về trạng thái oxy hóa của U, Fe và Mn trong trầm tích bị nhiễm U từ Phòng thí nghiệm quốc gia Oak Ridge. Trạng thái oxy hóa của các nguyên tố này được đo theo thời gian thực và không phá hủy bằng cách sử dụng phổ hấp thụ tia X trên các cột trầm tích được cung cấp nước ngầm tổng hợp có chứa cacbon hữu cơ (OC, 0, 3, 10, 30 và 100 mM OC dưới dạng lactat) trong hơn 400 ngày. Trong trầm tích được cung cấp OC> hoặc = 30 mM, 80% U đã bị khử thành U(IV), với sự oxy hóa lại tạm thời sau khoảng 150 ngày. Các oxit Mn(III,IV) đã bị khử hoàn toàn thành Mn(II) trong trầm tích được truyền OC> hoặc = 3 mM. Tuy nhiên, Fe vẫn phần lớn không bị khử trong tất cả các cột trầm tích, cho thấy rằng Fe(III) có thể tồn tại như một chất nhận electron trong quá trình khử trầm tích trong thời gian dài. Kết quả này kết hợp với sự khử hoàn toàn của tất cả các chất nhận electron tiềm năng khác hỗ trợ cho giả thuyết rằng phần Fe(III) phản ứng chịu trách nhiệm cho quá trình oxy hóa lại U(IV)."} +{"idx": 1970008, "en": "The debromination pathways of seven polybrominated diphenyl ethers (PBDEs) by three different cultures of anaerobic dehalogenating bacteria were investigated using comprehensive two-dimensional gas chromatography (GC x GC). The congeners analyzed were the five major components of the industrially used octa-BDE mixture (octa-BDEs 196, 203, and 197, hepta-BDE 183, and hexa-BDE 153) as well as the two most commonly detected PBDEs in the environment, penta-BDE 99 and tetra-BDE 47. Among the dehalogenating cultures evaluated in this study were a trichloroethene-enriched consortium containing multiple Dehalococcoides species, and two pure cultures, Dehalobacter restrictus PER-K23 and Desulfitobacterium hafniense PCP-1. PBDE samples were analyzed by GC x GC coupled to an electron capture detector to maximize separation and identification of the product congeners. All studied congeners were debrominated to some extent by the three cultures and all exhibited similar debromination pathways with preferential removal of para and meta bromines. Debromination of the highly brominated congeners was extremely slow, with usually less than 10% of nM concentrations of PBDEs transformed after three months. In contrast, debromination of the lesser brominated congeners, such as penta 99 and tetra 47, was faster, with some cultures completely debrominating nM levels of tetra 47 within weeks.", "gemini": "Các con đường khử brom của bảy ete diphênyl polybrom (PBDE) bởi ba loại vi khuẩn khử halogen kỵ khí khác nhau đã được nghiên cứu bằng phương pháp sắc ký khí hai chiều toàn diện (GC x GC). Các đồng phân được phân tích là năm thành phần chính của hỗn hợp octa-BDE được sử dụng trong công nghiệp (octa-BDE 196, 203 và 197, hepta-BDE 183 và hexa-BDE 153) cũng như hai PBDE được phát hiện phổ biến nhất trong môi trường, penta-BDE 99 và tetra-BDE 47. Trong số các loại vi khuẩn khử halogen được đánh giá trong nghiên cứu này có một nhóm giàu trichloroethene chứa nhiều loài Dehalococcoides và hai loại vi khuẩn tinh khiết, Dehalobacter restrictus PER-K23 và Desulfitobacterium hafniense PCP-1. Các mẫu PBDE được phân tích bằng GC x GC kết hợp với máy dò bắt điện tử để tối đa hóa quá trình tách và xác định các đồng phân sản phẩm. Tất cả các đồng phân được nghiên cứu đều bị khử brom ở một mức độ nào đó bởi ba loại vi khuẩn và tất cả đều biểu hiện các con đường khử brom tương tự với sự loại bỏ ưu tiên các brom para và meta. Quá trình khử brom của các đồng phân có độ brom hóa cao diễn ra cực kỳ chậm, thường dưới 10% nồng độ nM của PBDE được chuyển hóa sau ba tháng. Ngược lại, quá trình khử brom của các đồng phân có độ brom hóa thấp hơn, chẳng hạn như penta 99 và tetra 47, diễn ra nhanh hơn, với một số loại vi khuẩn khử brom hoàn toàn nồng độ nM của tetra 47 trong vài tuần."} +{"idx": 1970009, "en": "Existing toxicity data indicate that industrial-scale production of C60 fullerene poses a potential threat to the environment. Evaluating the environmental impact of C60 requires careful characterization of its physicochemical properties in the natural aqueous environment. Our study aims to determine the effects of aquatic natural organic matter (NOM) on the physicochemical properties of aqueous C60 nanoparticles, nC60. Stable nC60 suspensions were formed using three different solvent exchange protocols. They were thoroughly characterized for particle size, morphology, and electrophoretic mobility in the absence or presence of two model NOM components, Suwannee River humic acid and fulvic acid. NOM caused disaggregation of nC60 crystals and aggregates under typical solution conditions of natural water, leading to significant changes in particle size and morphology. Such effect increased with increasing NOM concentration. The changes in nC060 size and morphology strongly depended on the nC60 formation pathway. Results from this study indicate that NOM may play a critical role in the transport and toxicity of C60 in the natural aqueous environment.", "gemini": "Dữ liệu độc tính hiện có chỉ ra rằng việc sản xuất fullerene C60 ở quy mô công nghiệp gây ra mối đe dọa tiềm tàng đối với môi trường. Việc đánh giá tác động môi trường của C60 đòi hỏi phải mô tả cẩn thận các tính chất lý hóa của nó trong môi trường nước tự nhiên. Nghiên cứu của chúng tôi nhằm xác định tác động của vật chất hữu cơ tự nhiên trong nước (NOM) đến các tính chất lý hóa của các hạt nano C60 trong nước, nC60. Các huyền phù nC60 ổn định được hình thành bằng cách sử dụng ba giao thức trao đổi dung môi khác nhau. Chúng được mô tả đầy đủ về kích thước hạt, hình thái và độ di động điện di khi không có hoặc có hai thành phần NOM mẫu, axit humic sông Suwannee và axit fulvic. NOM gây ra sự tách rời của các tinh thể và tập hợp nC60 trong các điều kiện dung dịch điển hình của nước tự nhiên, dẫn đến những thay đổi đáng kể về kích thước và hình thái hạt. Hiệu ứng như vậy tăng lên khi nồng độ NOM tăng. Những thay đổi về kích thước và hình thái nC060 phụ thuộc rất nhiều vào con đường hình thành nC60. Kết quả từ nghiên cứu này chỉ ra rằng NOM có thể đóng vai trò quan trọng trong quá trình vận chuyển và độc tính của C60 trong môi trường nước tự nhiên."} +{"idx": 1970010, "en": "The competitive binding of Cd2+ and Zn2+ by the phytochelatin (gamma-Glu-Cys)4-Gly (PC4) has been examined using several techniques. Electrospray ionization mass spectrometry is used to determine the stoichiometries of the complexes, while voltammetric data analyzed by multivariate curve resolution with alternating least squares allows one to not only trace the displacement induced by Cd2+ or Zn2+ in the binding of Zn2+ or Cd2+, respectively, by PC4, but also to obtain a complete overview of the processes involved. Isothermal titration calorimetry is used to determine the related binding and thermodynamic parameters. Results obtained via these different techniques are compared and discussed below. The formation of ternary CdZn(PC4) and Cd2Zn(PC4) complexes was observed.", "gemini": "Sự liên kết cạnh tranh của Cd2+ và Zn2+ bởi phytochelatin (gamma-Glu-Cys)4-Gly (PC4) đã được kiểm tra bằng một số kỹ thuật. Phổ khối điện tử phun được sử dụng để xác định tỷ lượng của các phức chất, trong khi dữ liệu voltam được phân tích bằng độ phân giải đường cong đa biến với các bình phương tối thiểu xen kẽ cho phép người ta không chỉ theo dõi sự dịch chuyển do Cd2+ hoặc Zn2+ gây ra trong liên kết của Zn2+ hoặc Cd2+, tương ứng, bởi PC4, mà còn để có được cái nhìn tổng quan hoàn chỉnh về các quá trình liên quan. Nhiệt lượng kế đẳng nhiệt được sử dụng để xác định liên kết liên quan và các thông số nhiệt động lực học. Kết quả thu được thông qua các kỹ thuật khác nhau này được so sánh và thảo luận dưới đây. Sự hình thành của các phức hợp CdZn(PC4) và Cd2Zn(PC4) bậc ba đã được quan sát."} +{"idx": 1970011, "en": "Because Pb is one of the most toxic elements and is found as a major contaminant in mining environments, this study aims to identify the distribution of this element in host phases issued from the alteration of mine wastes. The sampling location was a former mine near Oakland, California (USA). This mine was once a source of sulfide minerals from which sulfuric acid was made. The material discussed in this paper was collected in iron hardpans that were formed within the waste rock pile resulting from the excavation work. In most contaminated environments (soils, mine waste), secondary metal-bearing phases arising from alteration processes are usually fine-grained (from 10 microm to less than 1 microm) and highly heterogeneous, requiring the use of micron-scale techniques. We performed micro-Raman spectroscopy, microscanning X-ray diffraction (SXRD), and microextended X-ray near edge spectroscopy (XANES) to determine the relationships between Pb and a Ba/Fe-rich host phase. Micro-Raman spectroscopy suggests that Pb is preferentially incorporated into barite rather than goethite. Results from micro-Raman experiments show the high sensitivity of this analytical tool to the incorporation of Pb into barite by being especially sensitive to the variations of the S-O bond and showing the characteristic bands due to the contribution of Pb. This association is confirmed and is well-illustrated by micro-SXRD mineral species maps showing the correlation between Pb and barite. Microfocused XANES indicates that Pb is present as Pb2+, agreeing with the in situ physicochemical parameters.", "gemini": "Vì Pb là một trong những nguyên tố độc hại nhất và được tìm thấy là chất gây ô nhiễm chính trong môi trường khai thác, nghiên cứu này nhằm xác định sự phân bố của nguyên tố này trong các pha chủ từ sự biến đổi của chất thải khai thác. Địa điểm lấy mẫu là một mỏ cũ gần Oakland, California (Hoa Kỳ). Mỏ này từng là nguồn cung cấp khoáng chất sunfua để sản xuất axit sunfuric. Vật liệu được thảo luận trong bài báo này được thu thập trong các lớp cứng sắt được hình thành bên trong đống đá thải do công tác khai thác. Trong hầu hết các môi trường bị ô nhiễm (đất, chất thải khai thác), các pha mang kim loại thứ cấp phát sinh từ quá trình biến đổi thường có hạt mịn (từ 10 microm đến nhỏ hơn 1 microm) và rất không đồng nhất, đòi hỏi phải sử dụng các kỹ thuật ở cấp độ micron. Chúng tôi đã thực hiện phổ Raman vi mô, nhiễu xạ tia X quét vi mô (SXRD) và phổ XANES gần cạnh mở rộng vi mô (XANES) để xác định mối quan hệ giữa Pb và pha chủ giàu Ba/Fe. Phổ Raman vi mô cho thấy Pb được kết hợp ưu tiên vào barit hơn là goethit. Kết quả từ các thí nghiệm Raman vi mô cho thấy độ nhạy cao của công cụ phân tích này đối với sự kết hợp của Pb vào barit bằng cách đặc biệt nhạy với các biến thể của liên kết S-O và cho thấy các dải đặc trưng do sự đóng góp của Pb. Sự liên kết này được xác nhận và được minh họa rõ ràng bằng bản đồ loài khoáng vật SXRD vi mô cho thấy mối tương quan giữa Pb và barit. XANES lấy nét vi mô chỉ ra rằng Pb có mặt dưới dạng Pb2+, phù hợp với các thông số lý hóa tại chỗ."} +{"idx": 1970012, "en": "Nanometer scale pores are ubiquitous in porous geologic media (soils and sediments). Sorption of organic contaminants in micropores (< or = 2 nm) can inhibittheir hydrolytic transformation due to the limited availability of reactive water within hydrophobic micropore spaces. As a test case, we studied the dehydrohalogenation of 2,2-dichloropropane (2,2-DCP) sorbed in the micropores of several model mineral solids. In the micropores of a hydrophobic dealuminated Y zeolite, CBV-780, 2,2-DCP dehydrohalogenation proceeded significantly slower than in bulk aqueous solution and eventually stopped. This was attributed to the depletion of reactive water molecules in the micropore spaces. The 2,2-DCP sorbed in the micropores of more hydrophilic solids (aquifer sediment, aquifer sand, and silica gel) also transformed slower than in aqueous solution, and the reaction no longer followed first-order kinetics. Results of transport modeling support that reactive contaminants sorbed in microporous minerals can be preserved over geological time scales under conditions that limit desorption. This study shows that hydrophobic micropores in geological media may act as an important sink for anthropogenic organic contaminants in the subsurface, and that sorption in micropores may significantly increase the persistence of the sorbed contaminants.", "gemini": "Các lỗ rỗng kích thước nanomet có ở khắp mọi nơi trong môi trường địa chất xốp (đất và trầm tích). Sự hấp phụ các chất gây ô nhiễm hữu cơ trong các lỗ rỗng nhỏ (< hoặc = 2 nm) có thể ức chế quá trình biến đổi thủy phân của chúng do khả năng tiếp cận hạn chế của nước phản ứng trong các không gian lỗ rỗng nhỏ kỵ nước. Là một trường hợp thử nghiệm, chúng tôi đã nghiên cứu quá trình khử hydrohalogen của 2,2-dicloropropan (2,2-DCP) được hấp phụ trong các lỗ rỗng nhỏ của một số khoáng chất mô hình rắn. Trong các lỗ rỗng nhỏ của zeolit Y đã khử nhôm kỵ nước, CBV-780, quá trình khử hydrohalogen 2,2-DCP diễn ra chậm hơn đáng kể so với trong dung dịch nước khối và cuối cùng dừng lại. Điều này được cho là do sự cạn kiệt các phân tử nước phản ứng trong các không gian lỗ rỗng nhỏ. 2,2-DCP được hấp phụ trong các lỗ rỗng nhỏ của các chất rắn ưa nước hơn (trầm tích tầng chứa nước, cát tầng chứa nước và silica gel) cũng biến đổi chậm hơn trong dung dịch nước, và phản ứng không còn tuân theo động học bậc nhất. Kết quả của mô hình vận chuyển hỗ trợ rằng các chất gây ô nhiễm phản ứng được hấp phụ trong các khoáng chất có lỗ rỗng nhỏ có thể được bảo toàn trong thang thời gian địa chất trong các điều kiện hạn chế sự giải hấp. Nghiên cứu này cho thấy các lỗ rỗng nhỏ kỵ nước trong môi trường địa chất có thể đóng vai trò là một bồn chứa quan trọng đối với các chất gây ô nhiễm hữu cơ do con người gây ra ở dưới bề mặt, và sự hấp phụ trong các lỗ rỗng nhỏ có thể làm tăng đáng kể sự tồn tại của các chất gây ô nhiễm được hấp phụ."} +{"idx": 1970013, "en": "The interaction of trace organic contaminants with bulk organic matter has implications for the transport and behavior of organic trace contaminants within the aquatic environment as well as water and wastewater treatment processes. Partition coefficients (K(OM)) of the steroidal trace organic contaminant estradiol were quantified for environmentally relevant concentrations of bulk organic matter (12.5 mg C/L) using a full mass balance form of solid-phase microextraction (SPME). The results indicated that the method is successful and can be used at environmental concentrations. Estradiol had the greatest affinity for bulk organic matter that contained phenolic and benzoic acid ester groups, namely tannic acid, compared to organics containing predominately carboxylic functional groups. The solution chemistry (pH) was found to influence the interaction, as estradiol had a lower affinity for negatively charged and hydrophilic bulk organic matter. The partition coefficients determined using SPMEwere consistentwith partition coefficients derived using solubility enhancement and fluorescence quenching measurements, confirming that SPME is a powerful technique to quantify the affinity of estradiol for low concentrations of bulk organic matter and trace contaminants. Further, this novel method can be applied to a range of trace contaminants.", "gemini": "Sự tương tác của các chất gây ô nhiễm hữu cơ dạng vết với vật chất hữu cơ khối có ý nghĩa đối với quá trình vận chuyển và hành vi của các chất gây ô nhiễm hữu cơ dạng vết trong môi trường nước cũng như các quy trình xử lý nước và nước thải. Các hệ số phân bố (K(OM)) của chất gây ô nhiễm hữu cơ dạng vết steroid estradiol đã được định lượng đối với nồng độ vật chất hữu cơ khối có liên quan đến môi trường (12,5 mg C/L) bằng cách sử dụng dạng cân bằng khối đầy đủ của phương pháp vi chiết pha rắn (SPME). Kết quả chỉ ra rằng phương pháp này thành công và có thể được sử dụng ở nồng độ môi trường. Estradiol có ái lực lớn nhất đối với vật chất hữu cơ khối có chứa các nhóm este axit phenolic và benzoic, cụ thể là axit tannic, so với các chất hữu cơ chủ yếu chứa các nhóm chức cacboxylic. Hóa học của dung dịch (pH) được phát hiện là ảnh hưởng đến sự tương tác, vì estradiol có ái lực thấp hơn đối với vật chất hữu cơ khối mang điện tích âm và ưa nước. Các hệ số phân bố xác định bằng SPME phù hợp với các hệ số phân bố thu được bằng cách sử dụng các phép đo tăng cường độ hòa tan và dập tắt huỳnh quang, xác nhận rằng SPME là một kỹ thuật mạnh mẽ để định lượng ái lực của estradiol đối với nồng độ thấp của vật chất hữu cơ khối và các chất gây ô nhiễm dạng vết. Hơn nữa, phương pháp mới này có thể được áp dụng cho nhiều chất gây ô nhiễm dạng vết."} +{"idx": 1970014, "en": "Single solute adsorption and coadsorption of As(III) and As(V) onto hydrous ferric oxide (HFO), oxidation of As(III), and extraction efficiencies were measured in 0.2 atm O2. Oxidation was negligible for single-adsorbate experiments, but significant oxidation was observed in the presence of As(V) and HFO. Single-adsorbate As(III) or As(V) were incompletely extracted (0.5 M NaOH for 20 min), but all As was recovered in coadsorbate experiments. Single-adsorbate data were well-simulated using published surface complexation models, but those models (calibrated for single-adsorbate results) provided poor fits for coadsorbate experiments. An amended model accurately simulated single- and coadsorbate results. Model predictions of significant change in As(III) surface complex speciation in coadsorbate experiments was confirmed using zeta potential measurements. Our results demonstrate that mobility of arsenic in groundwater and removal in engineered treatment systems are more complicated when both As(III) and As(V) are present than anticipated based on single-adsorbate experimental results.", "gemini": "Sự hấp phụ chất tan đơn và sự hấp phụ đồng thời của As(III) và As(V) lên oxit sắt ngậm nước (HFO), quá trình oxy hóa As(III) và hiệu suất chiết xuất đã được đo trong 0,2 atm O2. Quá trình oxy hóa không đáng kể đối với các thí nghiệm hấp phụ đơn chất, nhưng quá trình oxy hóa đáng kể đã được quan sát thấy khi có sự hiện diện của As(V) và HFO. As(III) hoặc As(V) hấp phụ đơn chất không được chiết xuất hoàn toàn (0,5 M NaOH trong 20 phút), nhưng tất cả As đều được thu hồi trong các thí nghiệm hấp phụ đồng thời. Dữ liệu hấp phụ đơn chất được mô phỏng tốt bằng cách sử dụng các mô hình phức hợp bề mặt đã công bố, nhưng các mô hình đó (hiệu chuẩn cho kết quả hấp phụ đơn chất) lại không phù hợp với các thí nghiệm hấp phụ đồng thời. Một mô hình đã sửa đổi mô phỏng chính xác kết quả hấp phụ đơn chất và đồng thời. Dự đoán của mô hình về sự thay đổi đáng kể trong quá trình hình thành phức hợp bề mặt As(III) trong các thí nghiệm hấp phụ đồng thời đã được xác nhận bằng cách sử dụng các phép đo điện thế zeta. Kết quả của chúng tôi chứng minh rằng tính di động của asen trong nước ngầm và quá trình loại bỏ trong các hệ thống xử lý kỹ thuật phức tạp hơn khi cả As(III) và As(V) đều có mặt so với dự đoán dựa trên kết quả thí nghiệm hấp phụ đơn chất."} +{"idx": 1970015, "en": "As greater quantities of carbon nanotubes (CNTs) enter the environment, they will have an increasingly important effect on the availability and transport of aqueous contaminants. As a consequence of purification, deliberate surface functionalization, and/or exposure to oxidizing agents after release to the environment, CNTs often contain surface oxides (i.e., oxygen containing functional groups). To probe the influence that surface oxides exert on CNT sorption properties, multiwalled CNTs (MWCNTs) with varying oxygen concentrations were studied with respect to their sorption properties toward naphthalene. For pristine (as-received) MWCNTs, the sorption capacity was intermediate between that of a natural char and a granular activated carbon. Sorption data also reveal that a linear relationship exists between the oxygen content of MWCNTs and their maximum adsorption capacity for naphthalene, with 10% surface oxygen concentration resulting in a roughly 70% decrease in maximum adsorption capacity. The relative distribution of sorption energies, as characterized by Freundlich isotherm exponents was, however, unaffected by oxidation. Thus, the data are consistent with the idea that incorporated surface oxides create polar regions that reduce the surface area available for naphthalene sorption. These results highlight the important role of surface chemistry in controlling the environmental properties of CNTs.", "gemini": "Khi một lượng lớn các ống nano các-bon (CNT) xâm nhập vào môi trường, chúng sẽ có tác động ngày càng quan trọng đến tính sẵn có và quá trình vận chuyển các chất gây ô nhiễm trong nước. Do quá trình tinh chế, chức năng hóa bề mặt có chủ đích và/hoặc tiếp xúc với các chất oxy hóa sau khi thải ra môi trường, CNT thường chứa các oxit bề mặt (tức là các nhóm chức năng chứa oxy). Để thăm dò ảnh hưởng của các oxit bề mặt lên các đặc tính hấp phụ của CNT, các CNT nhiều thành (MWCNT) có nồng độ oxy khác nhau đã được nghiên cứu liên quan đến các đặc tính hấp phụ của chúng đối với naphthalene. Đối với MWCNT nguyên chất (như đã nhận được), khả năng hấp phụ nằm ở mức trung bình giữa than tự nhiên và than hoạt tính dạng hạt. Dữ liệu hấp phụ cũng cho thấy có mối quan hệ tuyến tính giữa hàm lượng oxy của MWCNT và khả năng hấp phụ tối đa của chúng đối với naphthalene, với nồng độ oxy bề mặt 10% dẫn đến giảm khoảng 70% khả năng hấp phụ tối đa. Tuy nhiên, sự phân bố tương đối của năng lượng hấp phụ, được đặc trưng bởi các số mũ đẳng nhiệt Freundlich, không bị ảnh hưởng bởi quá trình oxy hóa. Do đó, dữ liệu phù hợp với ý tưởng rằng các oxit bề mặt được đưa vào tạo ra các vùng phân cực làm giảm diện tích bề mặt có sẵn để hấp phụ naphthalene. Những kết quả này làm nổi bật vai trò quan trọng của hóa học bề mặt trong việc kiểm soát các đặc tính môi trường của CNT."} +{"idx": 1970016, "en": "Cellulose degradation under alkaline conditions is of relevance to the mobility of many cations of the transition metal, lanthanide, and actinide series in the geosphere because strong complexants such as isosaccharinic acids, 3-deoxy-2-C-hydroxymethyl-D-erythro-pentonic acid (alpha-ISA) and 3-deoxy-2-C-hydroxymethyl-D-threo-pentonic acid (beta-ISA) may be formed. In the context of the long-term safety of cementitious repositories for low- and intermediate-level radioactive waste, where large amounts of cellulose may be present, the question of the time scales needed for the complete degradation of cellulose is important. The present paper reports the results of a 12 year study of the degradation of four different cellulosic materials (pure cellulose, tissue, cotton, paper) in an artificial cement pore water under anaerobic conditions at approximately 25 degrees C. The observed reaction characteristics can be divided into a fast reaction phase (2-3 years), dominated by the stepwise conversion of terminal glucose monomeric units to alpha-ISA and beta-ISA, and a very slow reaction phase during which the same products were found. The slow rate of the alkaline degradation of cellulose during this second reaction phase shows that previous kinetic models of cellulose degradation did not adequately describe the long-term behavior under alkaline conditions and need to be reassessed. It is postulated that a previously unknown mechanism by which crystalline or inaccessible reducing end groups of the polysaccharide chain become temporarily susceptible to alkaline attack is responsible for the slow rate of cellulose degradation.", "gemini": "Sự phân hủy xenluloza trong điều kiện kiềm có liên quan đến tính di động của nhiều cation của kim loại chuyển tiếp, lantanit và actinide trong địa cầu vì các chất phức mạnh như axit isosaccharinic, axit 3-deoxy-2-C-hydroxymethyl-D-erythro-pentonic (alpha-ISA) và axit 3-deoxy-2-C-hydroxymethyl-D-threo-pentonic (beta-ISA) có thể được hình thành. Trong bối cảnh an toàn lâu dài của các kho chứa xi măng cho chất thải phóng xạ mức độ thấp và trung bình, nơi có thể có nhiều xenluloza, thì câu hỏi về thang thời gian cần thiết để phân hủy hoàn toàn xenluloza là rất quan trọng. Bài báo hiện tại báo cáo kết quả của một nghiên cứu kéo dài 12 năm về sự phân hủy của bốn vật liệu xenluloza khác nhau (xenluloza tinh khiết, mô, bông, giấy) trong nước lỗ rỗng xi măng nhân tạo trong điều kiện yếm khí ở khoảng 25 độ C. Các đặc tính phản ứng quan sát được có thể được chia thành một pha phản ứng nhanh (2-3 năm), chi phối bởi quá trình chuyển đổi từng bước các đơn vị monome glucose đầu cuối thành alpha-ISA và beta-ISA, và một pha phản ứng rất chậm trong đó cùng các sản phẩm được tìm thấy. Tốc độ chậm của quá trình phân hủy xenluloza trong điều kiện kiềm trong pha phản ứng thứ hai này cho thấy các mô hình động học trước đây về sự phân hủy xenluloza không mô tả đầy đủ hành vi dài hạn trong điều kiện kiềm và cần được đánh giá lại. Người ta đưa ra giả thuyết rằng một cơ chế trước đây chưa được biết, theo đó các nhóm đầu khử tinh thể hoặc không tiếp cận được của chuỗi polysaccharide tạm thời dễ bị tấn công bởi kiềm, chịu trách nhiệm cho tốc độ phân hủy xenluloza chậm."} +{"idx": 1970017, "en": "The geochemistry of artificially metal contaminated sediments prepared using three methods of metal-spiking was compared in this study. Marine sediments with a gradient of Cu and Zn concentrations were prepared by direct-spiking without and with pH-adjustment to pH 7, and also by serial dilution of direct-spiked sediment (4000 microg g(-1), pH 7 adjusted) with uncontaminated sediment. Porewater concentrations of Cu, Zn, Fe, and Mn in direct-spiked sediments without pH adjustment were orders of magnitude higher than the equivalent sediments adjusted to pH 7 or those prepared by the serial dilution method. Despite pH-adjustment, porewater Cu and Zn concentrations of direct-spiked sediment remained higher than concentrations observed within metal-contaminated natural sediment. The serial dilution of metal-spiked, pH-adjusted sediment substantially decreased Cu and Zn partitioning to the dissolved phase, and minimized the variation of potential competitive ions (H+, Fe2+, Mn2+) over the entire gradient of spiked Cu and Zn concentrations. Metal concentration gradients created using serial dilution of Cu- and Zn-spiked, pH-adjusted sediments produced porewater Cu or Zn, Fe, and Mn concentrations that resemble sediment-porewater partitioning (Cu, Zn, Fe, Mn) typical of metal-contaminated natural sediments. This method is recommended for whole-sediment toxicology studies.", "gemini": "Trong nghiên cứu này, địa hóa học của trầm tích bị ô nhiễm kim loại nhân tạo được chế tạo bằng ba phương pháp tăng đột biến kim loại đã được so sánh. Trầm tích biển có nồng độ Cu và Zn theo dạng gradient đã được chế tạo bằng cách tăng đột biến trực tiếp mà không có và có điều chỉnh pH xuống pH 7, và cũng bằng cách pha loãng tuần tự trầm tích tăng đột biến trực tiếp (4000 microg g(-1), pH 7 đã điều chỉnh) với trầm tích không bị ô nhiễm. Nồng độ Cu, Zn, Fe và Mn trong nước lỗ rỗng ở trầm tích tăng đột biến trực tiếp mà không điều chỉnh pH cao hơn nhiều lần so với các trầm tích tương đương được điều chỉnh đến pH 7 hoặc những trầm tích được chế tạo bằng phương pháp pha loãng tuần tự. Mặc dù đã điều chỉnh pH, nồng độ Cu và Zn trong nước lỗ rỗng của trầm tích tăng đột biến trực tiếp vẫn cao hơn nồng độ quan sát được trong trầm tích tự nhiên bị ô nhiễm kim loại. Việc pha loãng tuần tự trầm tích được tăng đột biến kim loại, điều chỉnh pH làm giảm đáng kể sự phân bố Cu và Zn vào pha hòa tan và giảm thiểu sự thay đổi của các ion cạnh tranh tiềm tàng (H+, Fe2+, Mn2+) trên toàn bộ gradient nồng độ Cu và Zn tăng đột biến. Các gradient nồng độ kim loại được tạo ra bằng cách pha loãng tuần tự các trầm tích có tăng đột biến Cu và Zn, điều chỉnh pH tạo ra nồng độ Cu hoặc Zn, Fe và Mn trong nước lỗ rỗng giống với sự phân bố trầm tích-nước lỗ rỗng (Cu, Zn, Fe, Mn) điển hình của trầm tích tự nhiên bị ô nhiễm kim loại. Phương pháp này được khuyến nghị cho các nghiên cứu về độc tính của toàn bộ trầm tích."} +{"idx": 1970018, "en": "Lead oxide (PbO2) can be an important form of lead mineral scale occurring in some water distribution systems. It is believed to be formed by the oxidation of lead-containing plumbing materials by free chlorine. Its reactivity in water, however, has not been well studied. Iodide is also found in source drinking waters, albeit at low concentrations. Consideration of thermodynamics suggests that iodide can be oxidized by PbO2. In this investigation, iodide ion was used as a probe compound to study the reduction of PbO2 and the formation of iodoform, which has been predicted to be a carcinogen, in the presence of natural organic matter (NOM). The reduction of PbO2 by iodide can be expressed as PbO2 + 31(-) + 4H+ --> Pb(2+) + I3(-) + 2H2O, and the reaction kinetics has been determined in this study. In the presence of NOM, I3- reacts with NOM to form iodoform and its concentration is proportional to the NOM concentration. Our results indicate that PbO2 is a very powerful oxidant and can possibly serve as an oxidant reservoir for the formation of iodinated disinfection byproduct through a novel reaction pathway.", "gemini": "Oxit chì (PbO2) có thể là một dạng quan trọng của cặn khoáng chì xảy ra trong một số hệ thống phân phối nước. Người ta cho rằng nó được hình thành do quá trình oxy hóa các vật liệu ống nước chứa chì bằng clo tự do. Tuy nhiên, khả năng phản ứng của nó trong nước vẫn chưa được nghiên cứu kỹ. Iodua cũng được tìm thấy trong nguồn nước uống, mặc dù ở nồng độ thấp. Xem xét về nhiệt động lực học cho thấy iodua có thể bị oxy hóa bởi PbO2. Trong nghiên cứu này, ion iodua được sử dụng như một hợp chất thăm dò để nghiên cứu quá trình khử PbO2 và sự hình thành iodoform, được dự đoán là chất gây ung thư, khi có mặt chất hữu cơ tự nhiên (NOM). Phản ứng khử PbO2 bằng iodua có thể được biểu diễn như sau: PbO2 + 31(-) + 4H+ --> Pb(2+) + I3(-) + 2H2O và động học phản ứng đã được xác định trong nghiên cứu này. Khi có mặt NOM, I3- phản ứng với NOM để tạo thành iodoform và nồng độ của nó tỷ lệ thuận với nồng độ NOM. Kết quả của chúng tôi chỉ ra rằng PbO2 là một chất oxy hóa rất mạnh và có thể đóng vai trò như một nguồn dự trữ chất oxy hóa cho quá trình hình thành sản phẩm phụ khử trùng có chứa iod thông qua một con đường phản ứng mới."} +{"idx": 1970019, "en": "Environmental ranking of refrigerants is of need in many instances. The aim is to assess the relative environmental hazard posed by 40 refrigerants, including those used in the past, those presently used, and some proposed substitutes. Ranking is based upon ozone depletion potential, global warming potential, and atmospheric lifetime and is achieved by applying the Hasse diagram technique, a mathematical method that allows us to assess order relationships of chemicals. The refrigerants are divided into 13 classes, of which the chlorofluorocarbons, hydrofluorocarbons, hydrochlorofluorocarbons, hydrofluoroethers, and hydrocarbons contain the largest number of single substances. The dominance degree, a method for measuring order relationships among classes, is discussed and applied to the 13 refrigerant classes. The results show that some hydrofluoroethers are as problematic as the hydrofluorocarbons. Hydrocarbons and ammonia are the least problematic refrigerants with respect to the three environmental properties.", "gemini": "Xếp hạng tác nhân làm lạnh dựa trên các tiêu chí môi trường là điều cần thiết trong nhiều trường hợp. Mục đích là đánh giá mối nguy hiểm tương đối đối với môi trường do 40 chất làm lạnh gây ra, bao gồm cả những chất đã sử dụng trong quá khứ, những chất hiện đang sử dụng và một số chất thay thế được đề xuất. Xếp hạng dựa trên tiềm năng làm suy giảm tầng ozone, tiềm năng làm nóng toàn cầu và thời gian tồn tại trong khí quyển và được thực hiện bằng cách áp dụng kỹ thuật sơ đồ Hasse, một phương pháp toán học cho phép chúng ta đánh giá các mối quan hệ trật tự của các hóa chất. Các chất làm lạnh được chia thành 13 loại, trong đó các hợp chất cloflorocacbon, hợp chất hydroflorocacbon, hợp chất hydrochlorofluorocacbon, hợp chất hydrofluoroete và hydrocarbon chứa nhiều chất đơn nhất nhất. Mức độ chiếm ưu thế, một phương pháp để đo các mối quan hệ trật tự giữa các lớp, được thảo luận và áp dụng cho 13 lớp chất làm lạnh. Kết quả cho thấy một số hợp chất hydrofluoroete có vấn đề như các hợp chất hydrofluorocacbon. Hydrocarbon và amoniac là những chất làm lạnh ít có vấn đề nhất đối với ba đặc tính của môi trường."} +{"idx": 1970020, "en": "Light membranes composed of single-walled carbon nanotubes (SWNTs) can serve as efficient nanoscale vessels for encapsulation of tetrafluoromethane at 300 K and operating external pressure of 1 bar. We use grand canonical Monte Carlo simulation for modeling of CF4 encapsulation at 300 K and pressures up to 2 bar. We find that the amount of adsorbed CF4 strongly depends on the pore size in nanotubes; at 1 bar the most efficient nanotubes for volumetric storage have size R = 0.68 nm. This size corresponds to the (10,10) armchair nanotubes produced nowadays in large quantities. For mass storage (i.e., weight %) the most efficient nanotubes have size R = 1.02 nm corresponding to (15,15) armchair nanotubes. They are better adsorbents than currently used activated carbons and zeolites, reaching approximately equal to 2.4 mol kg(-1) of CF4, whereas, the best activated carbon Carbosieve G molecular sieve can adsorb 1.7 mol kg(-1) of CF4 at 300 K and 1 bar. We demonstrate that the high enthalpy of adsorption cannot be used as an only measure of storage efficiency. The optimal balance between the binding energy (i.e., enthalpy of adsorption) and space available for the accommodation of molecules (i.e., presence of inaccessible pore volume) is a key for encapsulation of van der Walls molecules. Our systematic computational study gives the clear direction in the timely problem of control emission of CF4 and other perfluorocarbons into atmosphere.", "gemini": "Các màng mỏng được tạo thành từ các ống nano cacbon thành một lớp (SWNT) có thể đóng vai trò như các bình chứa nano hiệu quả để đóng gói tetrafluorometan ở 300 K và áp suất bên ngoài hoạt động là 1 bar. Chúng tôi sử dụng mô phỏng Monte Carlo chuẩn lớn để mô hình hóa quá trình đóng gói CF4 ở 300 K và áp suất lên đến 2 bar. Chúng tôi thấy rằng lượng CF4 được hấp phụ phụ thuộc mạnh vào kích thước lỗ trong ống nano; ở 1 bar, các ống nano hiệu quả nhất để lưu trữ thể tích có kích thước R = 0,68 nm. Kích thước này tương ứng với các ống nano hình ghế bành (10,10) được sản xuất với số lượng lớn hiện nay. Đối với lưu trữ khối lượng (tức là trọng lượng %), các ống nano hiệu quả nhất có kích thước R = 1,02 nm tương ứng với các ống nano hình ghế bành (15,15). Chúng là chất hấp phụ tốt hơn so với than hoạt tính và zeolit hiện đang được sử dụng, đạt khoảng 2,4 mol kg(-1) CF4, trong khi đó, sàng phân tử than hoạt tính tốt nhất Carbosieve G có thể hấp phụ 1,7 mol kg(-1) CF4 ở 300 K và 1 bar. Chúng tôi chứng minh rằng nhiệt độ hấp phụ cao không thể được sử dụng làm thước đo duy nhất về hiệu quả lưu trữ. Sự cân bằng tối ưu giữa năng lượng liên kết (tức là nhiệt độ hấp phụ) và không gian có sẵn để chứa các phân tử (tức là sự hiện diện của thể tích lỗ không thể tiếp cận) là chìa khóa để đóng gói các phân tử van der Walls. Nghiên cứu tính toán có hệ thống của chúng tôi đưa ra hướng đi rõ ràng trong vấn đề kiểm soát phát thải CF4 và các perfluorocarbon khác vào khí quyển kịp thời."} +{"idx": 1970021, "en": "Abrasion tests were conducted using a brake dynamometer to determine the antimony (Sb) emission factor originating from automobiles. Abrasion dusts from commercially available brake pads (nonasbestos organic type) were emitted into an enclosed chamber under various braking conditions in terms of initial driving speed and deceleration. Suspended dusts inside the chamber were collected on a quartz fiber filter and weighed. From the experimental data, dust emission could be regressed as a function of the initial kinetic energy loading and the braking time. Using the regression function, the emission factors of brake abrasion dusts under the typical braking conditions (initial driving speed; 50 km/h, deceleration; 1.0 m/s2) were calculated as 5.8 mg/braking/car for PM10 and 3.9 mg/braking/car for PM2.5. The elemental composition of the collected dusts indicated that the fraction originating from disk wear contributed to approximately 30% of the suspended dusts. From these analytical results, it was concluded that the Sb emission factors originating from automobiles were approximately 32 microg Sb/braking/car for PM10 and 22 microg Sb/ braking/car for PM2.5. These essential data will contribute to the modeling of atmospheric Sb concentration alongside roadways and also to the better understanding of Sb source apportionment.", "gemini": "Các thử nghiệm mài mòn đã được tiến hành bằng cách sử dụng máy đo lực hãm để xác định hệ số phát thải antimon (Sb) có nguồn gốc từ ô tô. Bụi mài mòn từ má phanh thương mại (loại hữu cơ không amiăng) được phát thải vào một buồng kín trong các điều kiện phanh khác nhau về tốc độ lái ban đầu và độ giảm tốc. Bụi lơ lửng bên trong buồng được thu trên bộ lọc sợi thạch anh và cân. Từ dữ liệu thực nghiệm, lượng bụi phát thải có thể hồi quy theo hàm tải động năng ban đầu và thời gian phanh. Sử dụng hàm hồi quy, các hệ số phát thải của bụi mài mòn phanh trong các điều kiện phanh thông thường (tốc độ lái ban đầu; 50 km/h, độ giảm tốc; 1,0 m/s2) được tính là 5,8 mg/phanh/xe đối với PM10 và 3,9 mg/phanh/xe đối với PM2.5. Thành phần nguyên tố của bụi thu được chỉ ra rằng phần có nguồn gốc từ mài mòn đĩa đóng góp vào khoảng 30% bụi lơ lửng. Từ những kết quả phân tích này, có thể kết luận rằng các hệ số phát thải Sb có nguồn gốc từ ô tô xấp xỉ 32 microg Sb/phanh/xe đối với PM10 và 22 microg Sb/phanh/xe đối với PM2.5. Những dữ liệu thiết yếu này sẽ góp phần vào việc lập mô hình nồng độ Sb trong khí quyển dọc theo các tuyến đường và cũng giúp hiểu rõ hơn về sự phân bổ nguồn Sb."} +{"idx": 1970022, "en": "A dynamic snowpack module was implemented in the Danish Eulerian Hemispheric Model Persistant Organic Pollutants (DEHM-POP), an atmospheric chemistry-transport model designed to study the environmental fate of persistent organic pollutants in the Northern Hemisphere. The role of the snowpack on the fate of alpha-hexachlorocyclohexane (alpha-HCH) was investigated by making simulations both with and without the formation of a snowpack and comparing model results with data from 21 air monitoring sites. The inclusion of a dynamic snowpack module in the DEHM-POP model generally improves the fit between modeled and observed alpha-HCH air concentrations for the winter and spring seasons and the overall correlation coefficient between predicted and observed concentrations are improved at 8 of the sites. The predicted snowpack concentrations are in good agreement with the few available snow measurements from the Arctic. The presence of a snowpack increases surface boundary layer air concentrations of alpha-HCH at midlatitudes, while the effect is more pronounced in the Arctic due to the longer periods of snow cover. The results indicate that the snowpack module in DEHM-POP acts as a fast-exchanging temporary storage medium for alpha-HCH, as significant fractions were rapidly revolatilized back into the atmosphere following deposition with snowfall, although the current parametrization for vapor-exchange probably over emphasizes this process. Nonetheless, increased air concentrations observed between March and May (\"spring maximum events\"; SME) at several high latitude monitoring stations are also predicted by the model. The model results indicate that the SMEs are associated with the revolatilization of previously deposited chemical from the snowpack, following a reduction in the capacity of the snowpack to retain alpha-HCH with increasing temperatures toward the end of the winter period, rather than the actual melting of the snowpack. The SMEs are not predicted at all the Arctic monitoring sites by the model, and the significance of the snowpack in controlling these in the model is, therefore, open to question given the uncertainties in the snow-air partition coefficient (K(sa)) and the reliance of the model on a one-layer snowpack rather than a multilayered snowpack.", "gemini": "Một mô-đun lớp tuyết năng động đã được triển khai trong Mô hình ô nhiễm hữu cơ dai bán cầu Eulerian của Đan Mạch (DEHM-POP), một mô hình vận chuyển hóa học trong khí quyển được thiết kế để nghiên cứu số phận của ô nhiễm hữu cơ dai trong môi trường ở Bắc bán cầu. Vai trò của lớp tuyết đối với số phận của alpha-hexachlorocyclohexane (alpha-HCH) đã được nghiên cứu bằng cách thực hiện mô phỏng có và không có sự hình thành của lớp tuyết và so sánh kết quả mô hình với dữ liệu từ 21 địa điểm giám sát không khí. Việc đưa mô-đun lớp tuyết động vào mô hình DEHM-POP thường cải thiện sự phù hợp giữa nồng độ không khí alpha-HCH được mô hình hóa và quan sát được vào các mùa đông và xuân và hệ số tương quan tổng thể giữa nồng độ dự đoán và quan sát được cải thiện ở 8 địa điểm. Nồng độ lớp tuyết dự đoán phù hợp với một số ít phép đo tuyết có sẵn từ Bắc Cực. Sự hiện diện của lớp tuyết làm tăng nồng độ không khí của alpha-HCH ở tầng ranh giới bề mặt ở vĩ độ trung bình, trong khi hiệu ứng này rõ rệt hơn ở Bắc Cực do thời gian tuyết phủ dài hơn. Kết quả chỉ ra rằng mô-đun lớp tuyết trong DEHM-POP hoạt động như một môi trường lưu trữ tạm thời trao đổi nhanh đối với alpha-HCH, vì một phần đáng kể đã nhanh chóng được bay hơi trở lại bầu khí quyển sau khi lắng đọng cùng với tuyết rơi, mặc dù tham số hóa hiện tại để trao đổi hơi có thể nhấn mạnh quá mức quá trình này. Tuy nhiên, nồng độ không khí tăng được quan sát thấy từ tháng 3 đến tháng 5 (\"các sự kiện cực đại vào mùa xuân\"; SME) tại một số trạm giám sát ở vĩ độ cao cũng được mô hình dự đoán. Kết quả mô hình chỉ ra rằng các SME có liên quan đến sự bay hơi trở lại của hóa chất lắng đọng trước đó từ lớp tuyết, sau khi khả năng giữ lại alpha-HCH của lớp tuyết giảm do nhiệt độ tăng vào cuối thời kỳ mùa đông, chứ không phải do sự tan chảy thực tế của lớp tuyết. Các SME không được mô hình dự đoán tại tất cả các địa điểm giám sát ở Bắc Cực và do đó, tầm quan trọng của lớp tuyết trong việc kiểm soát các SME trong mô hình vẫn còn là câu hỏi mở do có sự không chắc chắn trong hệ số phân chia tuyết-không khí (K(sa)) và mô hình dựa vào lớp tuyết một lớp thay vì lớp tuyết nhiều lớp."} +{"idx": 1970023, "en": "In this work, total smooth air-water interfacial areas were measured for a series of nine natural and model sandy porous media as a function of water saturation using synchrotron X-ray microtomography. Interfacial areas decreased linearly with water satuation, while the estimated maximum interfacial area compared favorably to the media geometric surface areas. Importantly, relative interfacial area (i.e., normalized by geometric surface area) versus water saturation plots for all media collapsed into a single linear cluster (r2 = 0.93), suggesting that geometric surface area is an important, and perhaps sufficient, descriptor of sandy media that governs total smooth interfacial area-water saturation relationships. Measured relationships were used to develop an empirical model for estimating interfacial area-water saturation relationships for sandy porous media. Model-based interfacial area estimates for independent media were generally slightly higher than interfacial areas measured using aqueous-phase interfacial tracer methods, which may indicate that microtomography captures regions of the air-water interface that are not accessible to aqueous-phase interfacial tracers. The empirical model presented here requires only average particle diameter and porosity as input parameters and can be used to readily estimate air-water interfacial area-water saturation relationships for sandy porous media.", "gemini": "Trong công trình này, tổng diện tích giao diện không khí-nước mịn đã được đo cho một loạt chín loại phương tiện xốp cát tự nhiên và mô hình như một hàm của độ bão hòa nước bằng phương pháp chụp cắt lớp vi tính X quang đồng bộ. Diện tích giao diện giảm tuyến tính theo độ bão hòa nước, trong khi diện tích giao diện tối đa ước tính so sánh thuận lợi với diện tích bề mặt hình học của phương tiện. Quan trọng hơn, diện tích giao diện tương đối (tức là chuẩn hóa theo diện tích bề mặt hình học) so với các đồ thị độ bão hòa nước cho tất cả các phương tiện đã sụp đổ thành một cụm tuyến tính duy nhất (r2 = 0,93), cho thấy rằng diện tích bề mặt hình học là một mô tả quan trọng và có lẽ đủ của phương tiện cát chi phối tổng mối quan hệ diện tích giao diện mịn-độ bão hòa nước. Các mối quan hệ được đo đã được sử dụng để phát triển một mô hình thực nghiệm để ước tính các mối quan hệ diện tích giao diện-độ bão hòa nước cho các phương tiện xốp cát. Các ước tính diện tích giao diện dựa trên mô hình cho các phương tiện độc lập thường cao hơn một chút so với diện tích giao diện được đo bằng các phương pháp đánh dấu giao diện pha nước, điều này có thể chỉ ra rằng vi chụp cắt lớp ghi lại các vùng giao diện không khí-nước không thể tiếp cận được với các chất đánh dấu giao diện pha nước. Mô hình thực nghiệm được trình bày ở đây chỉ yêu cầu đường kính hạt trung bình và độ xốp làm tham số đầu vào và có thể được sử dụng để dễ dàng ước tính các mối quan hệ diện tích giao diện không khí-nước-độ bão hòa nước cho các phương tiện xốp cát."} +{"idx": 1970024, "en": "Using the pyrolysis-gas chromatography-mass spectrometry and off-line pyrolysis/silylation methods for lignites from three Miocene brown coal basins of Poland resulted in the characterization of many organic compounds, including dominant cellulose degradation products such as levoglucosan, 1,6-anhydro-beta-D-glucofuranose, and 1,4:3,6-dianhydroglucopyranose. Levoglucosan is a general source-specific tracer for wood smoke in the atmosphere and recent sediments. The presence of unusually high levels of this compound in brown coal pyrolysates suggests that a portion of this compound concentration in some airsheds may originate from lignite combustion. On the other hand, nonglucose anhydrosaccharides, in particular, mannosan and galactosan, typical of hemicellulose, are not detected in those lignite pyrolysates investigated. This indicates that mannosan and galactosan are better specific tracers for combustion of contemporary biomass in those regions were the utilization of brown coals containing fossilized cellulose is important.", "gemini": "Việc sử dụng phương pháp sắc ký khí nhiệt phân-phổ khối và phương pháp nhiệt phân/silyl hóa ngoại tuyến đối với lignit từ ba lưu vực than nâu Miocene của Ba Lan đã dẫn đến việc xác định đặc tính của nhiều hợp chất hữu cơ, bao gồm các sản phẩm phân hủy cellulose chiếm ưu thế như levoglucosan, 1,6-anhydro-beta-D-glucofuranose và 1,4:3,6-dianhydroglucopyranose. Levoglucosan là một chất chỉ thị đặc hiệu theo nguồn chung cho khói gỗ trong khí quyển và trầm tích gần đây. Sự hiện diện của nồng độ cao bất thường của hợp chất này trong các sản phẩm nhiệt phân than nâu cho thấy một phần nồng độ hợp chất này trong một số vùng không khí có thể có nguồn gốc từ quá trình đốt lignit. Mặt khác, các anhydrosaccharide không phải glucose, cụ thể là mannosan và galactosan, đặc trưng của hemicellulose, không được phát hiện trong các sản phẩm nhiệt phân lignit đã nghiên cứu. Điều này chỉ ra rằng mannosan và galactosan là các chất chỉ thị đặc hiệu tốt hơn cho quá trình đốt sinh khối đương đại ở những khu vực mà việc sử dụng than nâu chứa cellulose hóa thạch là quan trọng."} +{"idx": 1970025, "en": "The dry ice sowing experiment (DISE) consisted in adding dry ice to a lake and monitoring the subsequent evasion of carbon dioxide (CO2). DISE allowed us to evaluate two approaches commonly used for measuring aquatic CO2 diffusive fluxes: the boundary layer equation (BLE) from Cole and Caraco (1998) and a particular model of static chamber (SC). CO2 evasion measurements with both approaches were compared to CO2 mass budgets as a relative reference to define their recovery coefficients (p). p for the BLE and the SC over the whole measurement period were 101 +/- 14% and 115 +/- 56%, respectively. Results from discrete sampling intervals revealed that the BLE generally provided estimations in good agreement (80-130%) with the mass budgets during both daytime and nighttime. Variations in p for the BLE were related to wind speed and, consequently, piston velocity (k600). The SC overestimated CO2 evasion during daytime (149 +/- 39%), and underestimated it during nighttime (57 +/- 18%). Variations in p for the SC were related to k600, stemming mainly from the alteration of the air/ water temperature gradient.", "gemini": "Thí nghiệm gieo băng khô (DISE) bao gồm việc thêm băng khô vào hồ và theo dõi quá trình thoát khí carbon dioxide (CO2) sau đó. DISE cho phép chúng tôi đánh giá hai phương pháp thường được sử dụng để đo thông lượng khuếch tán CO2 dưới nước: phương trình lớp ranh giới (BLE) từ Cole và Caraco (1998) và một mô hình cụ thể của buồng tĩnh (SC). Các phép đo thoát khí CO2 bằng cả hai phương pháp đã được so sánh với ngân sách khối lượng CO2 như một tài liệu tham khảo tương đối để xác định hệ số thu hồi của chúng (p). p cho BLE và SC trong toàn bộ thời gian đo lần lượt là 101 +/- 14% và 115 +/- 56%. Kết quả từ các khoảng lấy mẫu rời rạc cho thấy BLE thường cung cấp các ước tính phù hợp tốt (80-130%) với ngân sách khối lượng vào cả ban ngày và ban đêm. Các biến thể trong p đối với BLE có liên quan đến tốc độ gió và do đó, tốc độ pít-tông (k600). SC đã ước tính quá cao lượng thoát khí CO2 vào ban ngày (149 +/- 39%) và ước tính thấp hơn vào ban đêm (57 +/- 18%). Các biến thể trong p đối với SC có liên quan đến k600, chủ yếu bắt nguồn từ sự thay đổi của gradien nhiệt độ không khí/nước."} +{"idx": 1970026, "en": "Even without access to the electrical grid, a flow-through sampler (FTS) can collect gaseous and particle-bound semivolatile organic compounds (SOCs) from large volumes of air by turning into the wind and having the wind blow through a porous sampling medium. To test its performance under field conditions, a FTS and a traditional pumped high volume air sampler, both using polyurethane foam (PUF) as sampling medium, were codeployed at the campus of the University of Toronto Scarborough from August 2006 to June 2007. Quantitative relationships between the wind speed outside the sampler and after passage through the PUF were established and allow the accurate estimation of sampling volumes under conditions of low and high wind speed. Polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) were quantified in the samples taken by both air samplers. Separate analysis of seven PUF disks arranged sequentially within the FTS, confirm that even relatively volatile SOCs do not experience serious break-through. Theoretical plate number analysis of the break-through curves yields an understanding of the effect of temperature and wind speed on FTS sampling efficiency, and reveals different behavior of gaseous and particle-bound-compounds on the PUF. Air concentrations of PCBs and PAHs obtained with the FTS compare favorably with those obtained by averaging the concentrations of several 24 h active high volume samples taken during the same time period.", "gemini": "Ngay cả khi không có quyền truy cập vào lưới điện, một máy lấy mẫu dòng chảy (FTS) vẫn có thể thu thập các hợp chất hữu cơ bán dễ bay hơi (SOC) dạng khí và dạng hạt từ lượng lớn không khí bằng cách quay theo hướng gió và để gió thổi qua môi trường lấy mẫu xốp. Để kiểm tra hiệu suất của nó trong điều kiện thực tế, một FTS và một máy lấy mẫu không khí thể tích cao được bơm theo cách truyền thống, cả hai đều sử dụng bọt polyurethane (PUF) làm môi trường lấy mẫu, đã được triển khai cùng lúc tại khuôn viên của Đại học Toronto Scarborough từ tháng 8 năm 2006 đến tháng 6 năm 2007. Các mối quan hệ định lượng giữa tốc độ gió bên ngoài máy lấy mẫu và sau khi đi qua PUF đã được thiết lập và cho phép ước tính chính xác thể tích lấy mẫu trong điều kiện tốc độ gió thấp và cao. Biphenyl polychlor (PCB) và hydrocarbon thơm đa vòng (PAH) đã được định lượng trong các mẫu lấy bằng cả hai máy lấy mẫu không khí. Phân tích riêng biệt bảy đĩa PUF được sắp xếp tuần tự trong FTS xác nhận rằng ngay cả các SOC tương đối dễ bay hơi cũng không bị phá vỡ nghiêm trọng. Phân tích số đĩa lý thuyết của các đường cong phá vỡ mang lại sự hiểu biết về ảnh hưởng của nhiệt độ và tốc độ gió đối với hiệu quả lấy mẫu FTS và cho thấy hành vi khác nhau của các hợp chất dạng khí và dạng hạt trên PUF. Nồng độ PCB và PAH trong không khí thu được bằng FTS tương đương với nồng độ thu được bằng cách tính trung bình nồng độ của một số mẫu thể tích cao hoạt động 24 giờ được lấy trong cùng thời gian."} +{"idx": 1970027, "en": "Two adsorbent solid phase microextraction (SPME) fibers, 70 microm Carbowax divinylbenzene (CW/DVB) and 65 microm polydimethylsiloxane divinylbenzene (PDMS/DVB), were selected for the analysis of several target analytes (phenols, phosphates, phthalates, polycyclic aromatic hydrocarbons, and chlorinated pesticides) identified by the USGS in surface waters. Detection limits for standards ranged from 0.1 to 1 ng/mL for the CW/ DVB fiber and 0.1 to 2 ng/mL for the PDMS/DVB fiber for 20 of the analytes. The remaining analytes were not extracted because their polarity precluded their partition to the solid phase of the SPMEfiber. Groundwater and treated water samples collected from wells in northern New Jersey were then sampled for the USGS analytes by the SPME method as well as a modified version of EPA 525.5 using C-18 bonded solid phase extraction columns. Nine of the USGS analytes-bisphenol A, bis(2-ethylhexyl) phthalate, butylated hydroxytoluene, butlyated hydroxyanisole, diethyltoulamide, diethyl phthalate, bis(2-ethylhexyl) adipate, 1,4-dichlorobenzene, and triphenyl phosphate-were detected in groundwater samples using the CW/ DVB fiber.", "gemini": "Hai sợi vi chiết rắn pha hấp phụ (SPME), Carbowax divinylbenzene (CW/DVB) 70 microm và polydimethylsiloxane divinylbenzene (PDMS/DVB) 65 microm, đã được chọn để phân tích một số chất phân tích mục tiêu (phenol, phosphat, phthalat, hydrocarbon thơm đa vòng và thuốc trừ sâu clo) do USGS xác định trong nước mặt. Giới hạn phát hiện đối với các tiêu chuẩn dao động từ 0,1 đến 1 ng/mL đối với sợi CW/DVB và 0,1 đến 2 ng/mL đối với sợi PDMS/DVB đối với 20 chất phân tích. Các chất phân tích còn lại không được chiết xuất vì tính phân cực của chúng ngăn cản sự phân chia của chúng vào pha rắn của sợi SPME. Các mẫu nước ngầm và nước đã xử lý được thu thập từ các giếng ở phía bắc New Jersey sau đó được lấy mẫu cho các chất phân tích của USGS bằng phương pháp SPME cũng như một phiên bản đã sửa đổi của EPA 525.5 sử dụng các cột chiết pha rắn liên kết C-18. Chín chất phân tích của USGS - bisphenol A, bis (2-ethylhexyl) phthalate, butylated hydroxytoluene, butlyated hydroxyanisole, diethyltoulamide, diethyl phthalate, bis (2-ethylhexyl) adipate, 1,4-dichlorobenzene và triphenyl phosphate - đã được phát hiện trong các mẫu nước ngầm sử dụng sợi CW/DVB."} +{"idx": 1970028, "en": "Tylosin is a widely used macrolide antibiotic for therapeutics and growth promotion in swine, beef cattle, and poultry production. Through various routes such as manure application, emission, inappropriate disposal, etc., tylosin enters the environment. The fate of tylosin in the environment is not yet fully understood. In this study, two photoreaction products of tylosin in water were identified as isotylosin A alcohol (E,Z) and isotylosin A aldol (E,Z). Tylosin A, B, C, D, isotylosin A alcohol, and isotylosin A aldol were purified, and immunological cross-reactivities of these tylosin-related compounds were tested with a specificity of 26% for tylosin B, 19% for tylosin C, 106% for tylosin D, 121% for isotylosin A alcohol, and 46% for isotylosin A aldol, compared to 100% for tylosin A. Competitive direct enzyme-linked immunosorbent assay (ELISA) for tylosin detection in water was compared with a high-performance liquid chromatography (HPLC) method by analyzing the same water samples from a study of tylosin dissipation in water. ELISA kits detect the other tylosin-related compounds besides tylosin A, which can result in differences in tylosin determination in water.", "gemini": "Tylosin là một loại kháng sinh macrolide được sử dụng rộng rãi để điều trị và thúc đẩy tăng trưởng ở lợn, gia súc thịt và gia cầm. Thông qua các con đường khác nhau như bón phân, phát thải, thải bỏ không đúng cách, v.v., tylosin xâm nhập vào môi trường. Số phận của tylosin trong môi trường vẫn chưa được hiểu đầy đủ. Trong nghiên cứu này, hai sản phẩm phản ứng quang của tylosin trong nước được xác định là isotylosin A alcohol (E, Z) và isotylosin A aldol (E, Z). Tylosin A, B, C, D, isotylosin A alcohol và isotylosin A aldol đã được tinh chế và phản ứng chéo miễn dịch của các hợp chất liên quan đến tylosin này đã được thử nghiệm với độ đặc hiệu là 26% đối với tylosin B, 19% đối với tylosin C, 106% đối với tylosin D, 121% đối với isotylosin A alcohol và 46% đối với isotylosin A aldol, so với 100% đối với tylosin A. Xét nghiệm miễn dịch liên kết enzyme trực tiếp cạnh tranh (ELISA) để phát hiện tylosin trong nước đã được so sánh với phương pháp sắc ký lỏng hiệu năng cao (HPLC) bằng cách phân tích cùng một mẫu nước từ một nghiên cứu về sự tiêu tán của tylosin trong nước. Bộ dụng cụ ELISA phát hiện các hợp chất liên quan đến tylosin khác ngoài tylosin A, có thể dẫn đến sự khác biệt trong việc xác định tylosin trong nước."} +{"idx": 1970029, "en": "Based on the theory of stochastic resonance, a new algorithm was presented to enhance the signal-to-noise ratio (SNR) of weak HPLC/UV signals of triclocarban in water samples with a new method for optimizing the system parameters. The method was applied to experimental weak signals of HPLC/ UV, which was embedded in the noise background, and the SNR was enhanced greatly. The algorithm was employed to detect triclocarban residue in water with solid-phase extraction--high-performance liquid chromatography. The limit of detection and the limit of quantification were improved from 10 ng L(-1) and 50 ng L(-1) to 1 ng L(-1) and 5 ng L(9-1), respectively. The results showed an excellent quantitative relationship between concentrations and chromatographic responses.", "gemini": "Dựa trên lý thuyết cộng hưởng ngẫu nhiên, một thuật toán mới đã được trình bày để tăng tỷ lệ tín hiệu trên nhiễu (SNR) của tín hiệu HPLC/UV yếu của triclocarban trong mẫu nước với phương pháp mới để tối ưu hóa các thông số hệ thống. Phương pháp này được áp dụng cho các tín hiệu yếu trong thí nghiệm của HPLC/UV, được nhúng trong nền nhiễu và SNR được tăng lên rất nhiều. Thuật toán đã được sử dụng để phát hiện dư lượng triclocarban trong nước bằng phương pháp chiết pha rắn - sắc ký lỏng hiệu năng cao. Giới hạn phát hiện và giới hạn định lượng đã được cải thiện từ 10 ng L(-1) và 50 ng L(-1) xuống còn 1 ng L(-1) và 5 ng L(9-1), tương ứng. Kết quả cho thấy mối quan hệ định lượng tuyệt vời giữa nồng độ và đáp ứng sắc ký."} +{"idx": 1970030, "en": "Diesel exhaust contains numerous toxic substances that show different modes of action such as triggering aryl hydrocarbon receptor (AhR)-mediated pathways. We investigated AhR-mediated activity of exhaust generated by a heavy-duty diesel engine operated with or without iron- or copper/iron-catalyzed diesel particulate filters (DPFs). AhR agonists were quantified using the DR-CALUX reporter gene assay (exposure of cells for 24 h). We found 54-60 ng 2,3,7,8-tetrachlorodibenzo-p-dioxin CALUX equivalents (TCDD-CEQs) per m3 of exhaust in unfiltered samples and 6-16 ng TCDD-CEQ m3 in DPF-treated samples. DPF applications decreased TCDD-CEQ concentrations by almost 90%. Concentrations of known AhR agonists were determined with GC/HRMS and converted to TCDD-CEQ concentrations using compound-specific relative potency values. The analyzed nine polycyclic aromatic hydrocarbons (PAHs) and the 172,3,7,8-chlorinated dibenzodioxins/furans (23,7,8-PCDD/Fs) contributed only marginally (0.6-1.6%) to the total agonist concentration. However, both DPFs also decreased concentrations of individual PAHs by 7(0-80%. Variation of the assay exposure time (8, 24, 48,72, and 96 h) revealed that AhR-mediated activity decreased over time and reached a plateau after 72 h, which was most likely due to biotransformation of AhR agonists by the exposed H4IIE cells. At the plateau, we measured 1-2 ng TCDD-CEQ m(-3) in both an unfiltered and a filtered exhaust sample. Our findings show that DPFs are a promising technology to detoxify diesel exhaust regarding compounds with AhR-mediated activity.", "gemini": "Khí thải động cơ diesel chứa nhiều chất độc hại có các cơ chế hoạt động khác nhau như kích hoạt các con đường do thụ thể hydrocarbon aryl (AhR) trung gian. Chúng tôi đã nghiên cứu hoạt động do AhR trung gian của khí thải do động cơ diesel hạng nặng tạo ra khi vận hành có hoặc không có bộ lọc hạt diesel (DPF) xúc tác bằng sắt hoặc đồng/sắt. Các chất chủ vận AhR được định lượng bằng xét nghiệm gen phóng viên DR-CALUX (tiếp xúc tế bào trong 24 giờ). Chúng tôi đã tìm thấy 54-60 ng 2,3,7,8-tetrachlorodibenzo-p-dioxin CALUX tương đương (TCDD-CEQ) trên m3 khí thải trong các mẫu không lọc và 6-16 ng TCDD-CEQ m3 trong các mẫu xử lý bằng DPF. Các ứng dụng DPF làm giảm nồng độ TCDD-CEQ gần 90%. Nồng độ của các chất chủ vận AhR đã biết được xác định bằng GC/HRMS và chuyển đổi thành nồng độ TCDD-CEQ bằng cách sử dụng các giá trị hiệu lực tương đối riêng cho từng hợp chất. Chín hydrocarbon thơm đa vòng (PAH) được phân tích và 172,3,7,8-chlorinated dibenzodioxin/furan (23,7,8-PCDD/F) chỉ đóng góp không đáng kể (0,6-1,6%) vào tổng nồng độ chất chủ vận. Tuy nhiên, cả hai DPF cũng làm giảm nồng độ của từng PAH từ 7 (0-80%. Sự thay đổi thời gian tiếp xúc xét nghiệm (8, 24, 48,72 và 96 giờ) cho thấy hoạt động do AhR trung gian giảm dần theo thời gian và đạt đến mức ổn định sau 72 giờ, điều này rất có thể là do quá trình chuyển hóa sinh học của các chất chủ vận AhR bởi các tế bào H4IIE tiếp xúc. Ở mức ổn định, chúng tôi đã đo được 1-2 ng TCDD-CEQ m(-3) trong cả mẫu khí thải đã lọc và chưa lọc. Những phát hiện của chúng tôi cho thấy DPF là một công nghệ đầy hứa hẹn để giải độc khí thải động cơ diesel liên quan đến các hợp chất có hoạt động do AhR trung gian."} +{"idx": 1970031, "en": "Previous field studies on in situ bioremediation of uranium-contaminated groundwater in an aquifer in Rifle, Colorado identified two distinct phases following the addition of acetate to stimulate microbial respiration. In phase I, Geobacter species are the predominant organisms, Fe(III) is reduced, and microbial reduction of soluble U(VI) to insoluble U(IV) removes uranium from the groundwater. In phase II, Fe(III) is depleted, sulfate is reduced, and sulfate-reducing bacteria predominate. Long-term monitoring revealed an unexpected third phase during which U(VI) removal continues even after acetate additions are stopped. All three of these phases were successfully reproduced in flow-through sediment columns. When sediments from the third phase were heat sterilized, the capacity for U(VI) removal was lost. In the live sediments U(VI) removed from the groundwater was recovered as U(VI) in the sediments. This contrasts to the recovery of U(IV) in sediments resulting from the reduction of U(VI) to U(IV) during the Fe(III) reduction phase in acetate-amended sediments. Analysis of 16S rRNA gene sequences in the sediments in which U(VI) was being adsorbed indicated that members of the Firmicutes were the predominant organisms whereas no Firmicutes sequences were detected in background sediments which did not have the capacity to sorb U(VI), suggesting that the U(VI) adsorption might be due to the presence of these living organisms or at least their intact cell components. This unexpected enhanced adsorption of U(VI) onto sediments following the stimulation of microbial growth in the subsurface may potentially enhance the cost effectiveness of in situ uranium bioremediation.", "gemini": "Các nghiên cứu thực địa trước đây về biện pháp phục hồi sinh học tại chỗ đối với nước ngầm bị nhiễm urani tại một tầng chứa nước ở Rifle, Colorado đã xác định hai giai đoạn riêng biệt sau khi bổ sung axetat để kích thích quá trình hô hấp của vi khuẩn. Trong giai đoạn I, loài Geobacter là sinh vật chiếm ưu thế, Fe(III) bị khử và quá trình khử vi sinh vật của U(VI) hòa tan thành U(IV) không hòa tan loại bỏ urani khỏi nước ngầm. Trong giai đoạn II, Fe(III) bị cạn kiệt, sunfat bị khử và vi khuẩn khử sunfat chiếm ưu thế. Theo dõi lâu dài cho thấy một giai đoạn thứ ba bất ngờ trong đó quá trình loại bỏ U(VI) vẫn tiếp tục ngay cả sau khi dừng bổ sung axetat. Cả ba giai đoạn này đều được tái tạo thành công trong các cột trầm tích dòng chảy. Khi các trầm tích từ giai đoạn thứ ba được khử trùng bằng nhiệt, khả năng loại bỏ U(VI) bị mất. Trong các trầm tích sống, U(VI) loại bỏ khỏi nước ngầm được thu hồi dưới dạng U(VI) trong các trầm tích. Điều này trái ngược với việc thu hồi U(IV) trong các trầm tích do quá trình khử U(VI) thành U(IV) trong giai đoạn khử Fe(III) trong các trầm tích được bổ sung axetat. Phân tích trình tự gen rRNA 16S trong các trầm tích mà U(VI) đang được hấp phụ chỉ ra rằng các thành viên của Firmicutes là sinh vật chiếm ưu thế trong khi không phát hiện thấy trình tự Firmicutes trong các trầm tích nền không có khả năng hấp phụ U(VI), điều này cho thấy sự hấp phụ U(VI) có thể là do sự hiện diện của các sinh vật sống này hoặc ít nhất là các thành phần tế bào còn nguyên vẹn của chúng. Sự hấp phụ U(VI) tăng cường bất ngờ này lên các trầm tích sau khi kích thích sự phát triển của vi khuẩn trong lòng đất có khả năng làm tăng hiệu quả về chi phí của biện pháp phục hồi sinh học urani tại chỗ."} +{"idx": 1970032, "en": "Cyanide is commonly found in electroplating, mining, coal gasification, and petroleum refining effluents, which require treatment before being discharged. Cyanide in effluents exists either as free cyanide or as a metal complex. The kinetics of the oxidation of weak-acid dissociable cyanides by an environmentally friendly oxidant, ferrate(VI) (Fe(VI)O4(2-), Fe(VI)), were studied as a function of pH (9.1-10.5) and temperature (15-45 degrees C) using a stopped-flow technique. The weak-acid dissociable cyanides were Cd(CN)4(2-) and Ni(CN)4(2-), and the rate-laws for the oxidation may be -d[Fe(VI)]/dt = k[Fe(VI)][M(CN)4(2-)]n where n = 0.5 and 1 for Cd(CN)4(2-) and Ni(CN)4(2-), respectively. The rates decreased with increasing pH and were mostly related to a decrease in concentration of the reactive protonated Fe(VI) species, HFeO4(-). The stoichiometries with Fe(VI) were determined to be: 4HFeO4(-) + M(CN)4(2-) + 6H2O --> 4Fe(OH)3 + M(2+) + 4NCO(-) + O2 + 4OH(-). Mechanisms are proposed that agree with the observed reaction rate-laws and stoichiometries of the oxidation of weak-acid dissociable cyanides by Fe(VI). Results indicate that Fe(VI) is effective in removing cyanide in coke oven plant effluent, where organics are also present.", "gemini": "Xyanua thường được tìm thấy trong mạ điện, khai thác mỏ, khí hóa than và nước thải lọc dầu, cần được xử lý trước khi thải ra ngoài. Xyanua trong nước thải tồn tại dưới dạng xyanua tự do hoặc phức hợp kim loại. Động học của quá trình oxy hóa các xyanua phân ly axit yếu bằng chất oxy hóa thân thiện với môi trường, ferrat(VI) (Fe(VI)O4(2-), Fe(VI)), đã được nghiên cứu như một hàm của pH (9,1-10,5) và nhiệt độ (15-45 độ C) sử dụng kỹ thuật dòng dừng. Các xyanua phân ly axit yếu là Cd(CN)4(2-) và Ni(CN)4(2-), và định luật tốc độ cho quá trình oxy hóa có thể là -d[Fe(VI)]/dt = k[Fe(VI)][M(CN)4(2-)]n trong đó n = 0,5 và 1 cho Cd(CN)4(2-) và Ni(CN)4(2-), tương ứng. Tốc độ giảm khi pH tăng và chủ yếu liên quan đến sự giảm nồng độ của các loài Fe(VI) được proton hóa phản ứng, HFeO4(-). Phương pháp xác định tỷ lượng với Fe(VI) được xác định là: 4HFeO4(-) + M(CN)4(2-) + 6H2O --> 4Fe(OH)3 + M(2+) + 4NCO(-) + O2 + 4OH(-). Các cơ chế được đề xuất phù hợp với định luật tốc độ phản ứng quan sát được và phương pháp xác định tỷ lượng của quá trình oxy hóa các xyanua phân ly axit yếu bằng Fe(VI). Kết quả chỉ ra rằng Fe(VI) có hiệu quả trong việc loại bỏ xyanua trong nước thải của nhà máy lò nung than cốc, nơi cũng có các chất hữu cơ."} +{"idx": 1970033, "en": "Coupling of methanogenic and methanotrophic catabolisms was performed in a single-stage technology equipped with a water electrolysis cell placed in the effluent recirculation loop. The electrolysis-generated hydrogen served as an electron donor for both bicarbonate reduction into CH4 and reductive dechlorination, while the O2 and CH4, supported the cometabolic oxidation of chlorinated intermediates left over by the tetrachloroethylene (PCE) transformation. The electrolytical methanogenic/methanotrophic coupled (eMaMoC) process was tested in a laboratory-scale setup at PCE loads ranging from 5 to 50 micromol/L(rx) x d (inlet concentrations from 4 to 11 mg/L), and at various hydraulic residence times (HRT). Degradation followed essentially a reductive dechlorination pathway from PCE to cis-1,2-dichloroethene (DCE), and an oxidative pathway from DCE to CO2. PCE reductive dechlorination to DCE was consistently over 98% while a maximum oxidative DCE mineralization of 89% was obtained at a load of 4.3 micromol PCE/ L(rx) x d and an HRT of 6 days. Controlling dissolved oxygen concentrations within a relatively low range (2-3 mg/L) seemed instrumental to sustain the overall degradation capacity. Degradation kinetics were further evaluated: the apparent half-saturation constant (K(s)) had to be set relatively high (29 microM) for the simulated data to best fit the experimental ones. In spite of such kinetic limitations, the eMaMoC system, while fueled by water electrolysis, was effective in building and sustaining a functional methanogenic/methanotrophic consortium capable of significant PCE mineralization in a single-stage process. Hence, degradation standards are within reach so long as the methanotrophic DCE-oxidizing potential, including substrate affinity, are optimized and HRT accordingly adjusted.", "gemini": "Sự kết hợp của quá trình dị hóa metan và metan dưỡng được thực hiện trong một công nghệ một giai đoạn được trang bị một tế bào điện phân nước đặt trong vòng tuần hoàn nước thải. Hiđrô sinh ra từ quá trình điện phân đóng vai trò là chất cho điện tử cho cả quá trình khử bicacbonat thành CH4 và khử clo, trong khi O2 và CH4 hỗ trợ quá trình oxy hóa cộng sinh của các chất trung gian có clo còn sót lại do quá trình chuyển hóa tetrachloroetylen (PCE). Quá trình kết hợp metan/metan dưỡng điện phân (eMaMoC) đã được thử nghiệm trong một thiết lập quy mô phòng thí nghiệm ở tải lượng PCE trong khoảng từ 5 đến 50 micromol/L(rx) x d (nồng độ đầu vào từ 4 đến 11 mg/L) và ở các thời gian lưu thủy lực (HRT) khác nhau. Sự phân hủy về cơ bản theo con đường khử clo từ PCE đến cis-1,2-dichloroetilen (DCE) và một con đường oxy hóa từ DCE đến CO2. Quá trình khử clo PCE thành DCE luôn trên 98% trong khi quá trình khoáng hóa DCE oxy hóa tối đa đạt 89% ở tải lượng 4,3 micromol PCE/ L(rx) x d và HRT là 6 ngày. Việc kiểm soát nồng độ oxy hòa tan trong phạm vi tương đối thấp (2-3 mg/L) dường như đóng vai trò quan trọng trong việc duy trì khả năng phân hủy tổng thể. Động học phân hủy được đánh giá thêm: hằng số bán bão hòa biểu kiến (K(s)) phải được đặt tương đối cao (29 microM) để dữ liệu mô phỏng phù hợp nhất với dữ liệu thực nghiệm. Bất chấp những hạn chế về động học như vậy, hệ thống eMaMoC, trong khi được cung cấp năng lượng bằng điện phân nước, vẫn có hiệu quả trong việc xây dựng và duy trì một hợp quần metan/metan dưỡng chức năng có khả năng khoáng hóa PCE đáng kể trong một quy trình một giai đoạn. Do đó, các tiêu chuẩn phân hủy nằm trong tầm với miễn là tiềm năng oxy hóa DCE metan dưỡng, bao gồm ái lực với chất nền, được tối ưu hóa và HRT được điều chỉnh cho phù hợp."} +{"idx": 1970034, "en": "This paper presents for the first time the investigation of TiO2 photocatalysis for the removal of pesticides in gas phase. Dichlorvos was used as a model pesticide, and experiments were carried out using both static and dynamic reaction systems to explore the different aspects of the process. Thus, adsorption, reaction kinetics, and the influence of several operational parameters such as relative humidity (RH), inlet concentration, flow rate, and association of TiO2 with activated carbon (AC) were all examined in detail. Furthermore, a special attention was devoted to the analysis of reaction products by means of various analytical techniques such as Fourier transform infrared spectroscopy, automated thermal desorption technique coupled to gas chromatography-mass spectrometry instrument, gas chromatography equipped with a pulse discharge helium photoionization detector, and ion chromatography. The results showed an immediate and total removal of dichlorvos at ppbv levels (50-350 ppbv) along with a high mineralization extent (50-85%) into harmless final products (CO2, HCl, PO43-). Moreover, RH was found to significantly affectthe mineralization extent and the formation of reaction intermediates. On the basis of identification data, direct charge transfer and chlorine radical (Cl*) attack were shown to play a key role in the reaction mechanism at low RH, whereas at high RH, HO* radicals were the predominant active species.", "gemini": "Bài báo này lần đầu tiên trình bày nghiên cứu về quang xúc tác TiO2 để loại bỏ thuốc trừ sâu ở pha khí. Dichlorvos được sử dụng như một loại thuốc trừ sâu mô hình và các thí nghiệm được thực hiện bằng cách sử dụng cả hệ thống phản ứng tĩnh và động để khám phá các khía cạnh khác nhau của quá trình. Do đó, sự hấp phụ, động học phản ứng và ảnh hưởng của một số thông số vận hành như độ ẩm tương đối (RH), nồng độ đầu vào, tốc độ dòng chảy và sự kết hợp của TiO2 với than hoạt tính (AC) đều được kiểm tra chi tiết. Hơn nữa, một sự chú ý đặc biệt đã được dành cho việc phân tích các sản phẩm phản ứng bằng các kỹ thuật phân tích khác nhau như phổ hồng ngoại biến đổi Fourier, kỹ thuật giải hấp nhiệt tự động kết hợp với thiết bị sắc ký khí-phổ khối, sắc ký khí được trang bị máy dò ion hóa quang phổ helium xung và sắc ký ion. Kết quả cho thấy sự loại bỏ ngay lập tức và hoàn toàn dichlorvos ở mức ppbv (50-350 ppbv) cùng với mức độ khoáng hóa cao (50-85%) thành các sản phẩm cuối vô hại (CO2, HCl, PO43-). Hơn nữa, RH được phát hiện có ảnh hưởng đáng kể đến mức độ khoáng hóa và sự hình thành các chất trung gian phản ứng. Trên cơ sở dữ liệu nhận dạng, quá trình truyền điện tích trực tiếp và sự tấn công của gốc clo (Cl*) đã được chứng minh đóng vai trò chính trong cơ chế phản ứng ở RH thấp, trong khi ở RH cao, gốc HO* là loài hoạt động chiếm ưu thế."} +{"idx": 1970035, "en": "Addition of molasses and urea was tested as a means of stimulating microbial urea hydrolysis in the Eastern Snake River Plain Aquifer in Idaho. Ureolysis is an integral component of a novel remediation approach for divalent trace metal and radionuclide contaminants in groundwater and associated geomedia, where the contaminants are immobilized by coprecipitation in calcite. Generation of carbonate alkalinity from ureolysis promotes calcite precipitation. In calcite-saturated aquifers, this represents a potential long-term contaminant sequestration mechanism. In a single-well experiment, dilute molasses was injected three times over two weeks to promote overall microbial growth, followed by one urea injection. With molasses addition, total cell numbers in the groundwater increased 1-2 orders of magnitude. Estimated ureolysis rates in recovered groundwater samples increased from < 0.1 to > 25 nmol L(-1) hr(-1). A quantitative PCR assay for the bacterial ureC gene indicated that urease gene numbers increased up to 170 times above pre-injection levels. Following urea injection, calcite precipitates were recovered. Estimated values for an in situ first order ureolysis rate constant ranged from 0.016 to 0.057 d(-1). Although collateral impacts such as reduced permeability were observed, overall results indicated the viability of manipulating biogeochemical processes to promote contaminant sequestration.", "gemini": "Việc bổ sung mật mía và urê đã được thử nghiệm như một phương tiện kích thích quá trình thủy phân urê của vi khuẩn trong tầng chứa nước Eastern Snake River Plain ở Idaho. Ureolysis là một thành phần không thể thiếu của một phương pháp khắc phục mới đối với các chất gây ô nhiễm kim loại vết có hóa trị hai và chất phóng xạ trong nước ngầm và địa hóa liên quan, trong đó các chất gây ô nhiễm được cố định bằng cách đồng kết tủa trong canxit. Sự tạo ra tính kiềm cacbonat từ ureolysis thúc đẩy quá trình kết tủa canxit. Trong các tầng chứa nước bão hòa canxit, đây đại diện cho một cơ chế cô lập chất gây ô nhiễm tiềm tàng lâu dài. Trong một thí nghiệm một giếng, mật mía pha loãng được tiêm ba lần trong hai tuần để thúc đẩy sự phát triển chung của vi khuẩn, sau đó là một lần tiêm urê. Với việc bổ sung mật mía, tổng số tế bào trong nước ngầm tăng 1-2 bậc độ lớn. Tốc độ ureolysis ước tính trong các mẫu nước ngầm thu hồi tăng từ < 0,1 lên> 25 nmol L(-1) giờ(-1). Một xét nghiệm PCR định lượng đối với gen ureC của vi khuẩn cho thấy số lượng gen urease tăng tới 170 lần so với mức trước khi tiêm. Sau khi tiêm urê, các kết tủa canxit đã được thu hồi. Các giá trị ước tính cho hằng số tốc độ ureolysis bậc nhất tại chỗ dao động từ 0,016 đến 0,057 d(-1). Mặc dù các tác động phụ như giảm độ thấm đã được quan sát thấy, nhưng kết quả tổng thể cho thấy tính khả thi của việc thao tác các quá trình địa hóa sinh để thúc đẩy quá trình cô lập chất gây ô nhiễm."} +{"idx": 1970036, "en": "Microporous coconut-based activated carbon was impregnated with solutions of ammonium metatungstate or ammonium molybdate and then calcined in air in order to convert the salts into their corresponding oxides. The surface of those materials was characterized using adsorption of nitrogen, potentiometric titration, Fourier-transform infrared spectroscopy, X-ray diffraction, and thermal analysis. The results indicated a significant increase in surface acidity related to the presence of tungsten or molybdenum oxides. On the materials obtained, adsorption of ammonia from either dry or moist air was carried out. The oxides distributed on the surface provided Lewis and/or Brønsted centers for interactions with ammonia molecules or ammonium ions. Water on the surface of carbon or in the gas phase increased the amount of ammonia adsorbed via involvement of Brønsted-type interactions and/or by leading to the formation of molybdate or tungstate salts on the surface. Although the amount of ammonia adsorbed is closely related to the number of moles of oxides and their acidic centers, the carbon surface also contributes to the adsorption via providing small pores where ammonia can be dissolved in the water film.", "gemini": "Than hoạt tính xốp có nguồn gốc từ dừa được tẩm dung dịch amoni metatungstat hoặc amoni molybdat và sau đó được nung trong không khí để chuyển các muối thành các oxit tương ứng. Bề mặt của những vật liệu đó được đặc trưng bằng cách hấp phụ nitơ, chuẩn độ điện thế, quang phổ hồng ngoại biến đổi Fourier, nhiễu xạ tia X và phân tích nhiệt. Kết quả cho thấy sự gia tăng đáng kể về độ axit bề mặt liên quan đến sự hiện diện của các oxit vonfram hoặc molypden. Trên các vật liệu thu được, quá trình hấp phụ amoniac từ không khí khô hoặc ẩm đã được thực hiện. Các oxit phân bố trên bề mặt cung cấp các trung tâm Lewis và/hoặc Brønsted để tương tác với các phân tử amoniac hoặc ion amoni. Nước trên bề mặt cacbon hoặc trong pha khí làm tăng lượng amoniac được hấp phụ thông qua sự tham gia của các tương tác kiểu Brønsted và/hoặc bằng cách dẫn đến sự hình thành các muối molybdat hoặc tungstat trên bề mặt. Mặc dù lượng amoniac được hấp phụ có liên quan chặt chẽ đến số mol oxit và các trung tâm axit của chúng, nhưng bề mặt cacbon cũng góp phần vào quá trình hấp phụ thông qua việc cung cấp các lỗ nhỏ nơi amoniac có thể hòa tan trong màng nước."} +{"idx": 1970037, "en": "An emerging technology for the removal of N-nitrosodimethylamine (NDMA) from drinking and groundwater is reductive destruction using noble metal catalysts and hydrogen gas as a reducing agent. Bimetallic palladium-indium (Pd-In) supported on alumina combines the ability of Into activate NDMA with the hydrogen activating properties of Pd. This study examined the effect of In addition to a commercial 5% Pd by weight on gamma-Al2O3 catalyst on the efficacy of NDMA reduction. The pseudo-first-order rate constant increased proportionately to In loading from 0.057 h(-1) for 0% In to a maximum of 0.25 h(-1) for 1% In and then decreased with additional in loading. Data suggest that hydrogen activation occurred only on Pd surfaces and In activated NDMA 20 times more effectively than Pd on a mass basis. The rate-limiting factor was NDMA activation for In loadings below 1%. The decrease at higher loadings is interpreted as In blocking pore spaces and limiting access to Pd sites, suggesting monatomic hydrogen limitation. The only products detected were dimethylamine and ammonium with carbon and nitrogen balances in excess of 92%, consistent with a mechanism involving reductive N-N bond cleavage. Results from this study serve as a basis for optimizing bimetallic catalysts for treating NDMA contaminated waters.", "gemini": "Một công nghệ mới nổi để loại bỏ N-nitrosodimethylamine (NDMA) khỏi nước uống và nước ngầm là phá hủy khử bằng chất xúc tác kim loại quý và khí hydro làm chất khử. Palađi-indi (Pd-In) kim loại kép được hỗ trợ trên alumina kết hợp khả năng của Into kích hoạt NDMA với các tính chất hoạt hóa hydro của Pd. Nghiên cứu này đã kiểm tra tác dụng của việc bổ sung In vào chất xúc tác gamma-Al2O3 5% Pd theo trọng lượng đối với hiệu quả khử NDMA. Hằng số tỷ lệ phản ứng bậc pseudo thứ nhất tăng tỷ lệ thuận với lượng In nạp từ 0,057 h(-1) đối với 0% In đến tối đa 0,25 h(-1) đối với 1% In và sau đó giảm khi nạp thêm In. Dữ liệu cho thấy quá trình hoạt hóa hydro chỉ xảy ra trên bề mặt Pd và In kích hoạt NDMA hiệu quả hơn Pd 20 lần theo khối lượng. Yếu tố giới hạn tốc độ là quá trình hoạt hóa NDMA đối với lượng In nạp dưới 1%. Sự giảm ở tải trọng cao hơn được giải thích là In chặn các khoảng trống của lỗ và hạn chế khả năng tiếp cận các vị trí Pd, cho thấy sự hạn chế của hydro đơn nguyên tử. Các sản phẩm duy nhất được phát hiện là dimetylamin và amoni với cân bằng cacbon và nitơ vượt quá 92%, phù hợp với cơ chế liên quan đến sự cắt đứt liên kết N-N khử. Kết quả từ nghiên cứu này làm cơ sở để tối ưu hóa chất xúc tác kim loại kép để xử lý nước bị nhiễm NDMA."} +{"idx": 1970038, "en": "Adverse selection is a significant contributor to market failure in secondary personal computer (PC) markets. Signaling can act as a potential solution to adverse selection and facilitate superior remarketing of second-hand PCs. Signaling is a means whereby usage information can be utilized to enhance consumer perception of both value and utility of used PCs and, therefore, promote lifetime extension for these systems. This can help mitigate a large portion of the environmental impact associated with PC system manufacture. In this paper, the computer buying and selling behavior of consumers is characterized via a survey of 270 Irish residential users. Results confirm the existence of adverse selection in the Irish market with 76% of potential buyers being unwilling to purchase and 45% of potential vendors being unwilling to sell a used PC. The so-called \"closet affect\" is also apparent with 78% of users storing their PC after use has ceased. Results also indicate that consumers place a higher emphasis on specifications when considering a second-hand purchase. This contradicts their application needs which are predominantly Internet and word-processing/spreadsheet/presentation applications, 88% and 60% respectively. Finally, a market solution utilizing self monitoring and reporting technology (SMART) sensors for the purpose of real time usage monitoring is proposed, that can change consumer attitudes with regard to second-hand computer equipment.", "gemini": "Lựa chọn bất lợi là một yếu tố quan trọng góp phần vào sự thất bại của thị trường trong các thị trường máy tính cá nhân (PC) thứ cấp. Tín hiệu có thể đóng vai trò là một giải pháp tiềm năng cho việc lựa chọn bất lợi và tạo điều kiện tiếp thị lại vượt trội cho các PC đã qua sử dụng. Tín hiệu là một phương tiện mà thông tin sử dụng có thể được sử dụng để nâng cao nhận thức của người tiêu dùng về cả giá trị và tiện ích của PC đã qua sử dụng và do đó, thúc đẩy việc kéo dài tuổi thọ cho các hệ thống này. Điều này có thể giúp giảm thiểu một phần lớn tác động môi trường liên quan đến việc sản xuất hệ thống PC. Trong bài báo này, hành vi mua và bán máy tính của người tiêu dùng được mô tả thông qua một cuộc khảo sát 270 người dùng dân cư ở Ireland. Kết quả xác nhận sự tồn tại của việc lựa chọn bất lợi trên thị trường Ireland với 76% người mua tiềm năng không muốn mua và 45% người bán tiềm năng không muốn bán PC đã qua sử dụng. Cái gọi là \"hiệu ứng tủ quần áo\" cũng rõ ràng khi 78% người dùng lưu trữ PC của họ sau khi ngừng sử dụng. Kết quả cũng chỉ ra rằng người tiêu dùng chú trọng nhiều hơn vào các thông số kỹ thuật khi cân nhắc mua hàng đã qua sử dụng. Điều này trái ngược với nhu cầu ứng dụng của họ, chủ yếu là các ứng dụng Internet và xử lý văn bản/bảng tính/trình bày, lần lượt là 88% và 60%. Cuối cùng, một giải pháp thị trường sử dụng các cảm biến công nghệ tự giám sát và báo cáo (SMART) cho mục đích giám sát mức sử dụng thời gian thực được đề xuất, có thể thay đổi thái độ của người tiêu dùng đối với thiết bị máy tính đã qua sử dụng."} +{"idx": 1970039, "en": "Living plants transport substantial amounts of organic material into the soil. This process, called rhizodeposition, provides the substrate for the rhizospheric microbial community. In this study, a laboratory-scale sediment microbial fuel cell, of which the anode is positioned in the rhizosphere of the rice plants, is used to microbially oxidize the plant-derived organics. An electrical current was generated through the in situ oxidation of rhizodeposits from living rice plants. The electrical power output of a sediment microbial fuel cell was found to be a factor 7 higher in the presence of actively growing plants. This process offers the potential of light-driven power generation from living plants in a nondestructive way. Sustainable power productions up to 330 W ha(-1) could be attributed to the oxidation of the plant-derived compounds.", "gemini": "Cây sống vận chuyển một lượng lớn vật chất hữu cơ vào đất. Quá trình này, được gọi là lắng đọng rễ, cung cấp chất nền cho cộng đồng vi khuẩn rễ. Trong nghiên cứu này, một tế bào nhiên liệu vi sinh vật trầm tích ở quy mô phòng thí nghiệm, trong đó cực dương được đặt ở vùng rễ của cây lúa, được sử dụng để oxy hóa vi sinh vật các chất hữu cơ có nguồn gốc từ thực vật. Một dòng điện được tạo ra thông qua quá trình oxy hóa tại chỗ các chất lắng đọng rễ từ cây lúa sống. Công suất điện của một tế bào nhiên liệu vi sinh vật trầm tích được phát hiện cao hơn gấp 7 lần khi có sự phát triển mạnh mẽ của cây. Quá trình này mang lại tiềm năng tạo ra năng lượng từ ánh sáng từ cây sống theo cách không phá hủy. Sản lượng điện bền vững lên đến 330 W ha(-1) có thể được quy cho quá trình oxy hóa các hợp chất có nguồn gốc từ thực vật."} +{"idx": 1970040, "en": "This paper presents advantages of using a boron-doped diamond (BDD) electrode for hydrogen production and wastewater treatment in a single electrochemical cell. Results indicated that the BDD electrode possessed the widest known electrochemical window, allowing new possibilities for both anodic and cathodic reactions to simultaneously take place. The BDD electrode exhibited high anodic potential, generating high oxidation state radicals that facilitated oxidation of toxic waste organic compounds such as 4-nitrophenols. In contrast, because of widening of potential windows, the rate of hydrogen evolution at the cathode was significantly increased. Time-on-stream concentrations of reaction intermediates were monitored to elucidate mechanism involved in 4-nitrophenol oxidation. Spalling, fouling, or reduction in the thickness of thin-film diamond coating was not observed. Overall, the BDD electrode exhibits unique properties including chemical inertness, anticorrosion, and extended service life. These properties are especially important in wastewater treatment. Economic advantages were attributed to the low cost and long duration BDD electrode and the valuable hydrogen byproduct produced. Analysis has shown that technology associated with the BDD electrode could be effectively implemented with minimum energy input and capital requirements. When combined with solar energy and fuel cells, electrochemical wastewater processing can become energy efficient and cost-effective.", "gemini": "Bài báo này trình bày những lợi thế của việc sử dụng điện cực kim cương pha tạp bo (BDD) để sản xuất hydro và xử lý nước thải trong một tế bào điện hóa đơn. Kết quả chỉ ra rằng điện cực BDD sở hữu cửa sổ điện hóa rộng nhất đã biết, cho phép khả năng mới cho cả phản ứng anot và catot diễn ra đồng thời. Điện cực BDD thể hiện điện thế anot cao, tạo ra các gốc trạng thái oxy hóa cao tạo thuận lợi cho quá trình oxy hóa các hợp chất hữu cơ độc hại như 4-nitrophenol. Ngược lại, do cửa sổ điện thế mở rộng, tốc độ giải phóng hydro ở catot tăng lên đáng kể. Nồng độ trung gian phản ứng theo thời gian được theo dõi để làm sáng tỏ cơ chế liên quan đến quá trình oxy hóa 4-nitrophenol. Không quan sát thấy hiện tượng bong tróc, bám bẩn hoặc giảm độ dày của lớp phủ kim cương màng mỏng. Nhìn chung, điện cực BDD thể hiện các tính chất độc đáo bao gồm trơ về mặt hóa học, chống ăn mòn và tuổi thọ cao. Những tính chất này đặc biệt quan trọng trong xử lý nước thải. Những lợi thế về mặt kinh tế là do chi phí thấp và thời gian sử dụng lâu dài của điện cực BDD và sản phẩm phụ hydro có giá trị được tạo ra. Phân tích đã chỉ ra rằng công nghệ liên quan đến điện cực BDD có thể được triển khai hiệu quả với mức đầu tư vốn và năng lượng đầu vào tối thiểu. Khi kết hợp với năng lượng mặt trời và tế bào nhiên liệu, quá trình xử lý nước thải điện hóa có thể trở nên hiệu quả về năng lượng và tiết kiệm chi phí."} +{"idx": 1970041, "en": "The thermal decomposition of CO2 to CO and O2 is a potential route for the consumption and utilization of CO2. However, this reaction is limited by both the thermodynamic equilibrium and the kinetic barrier. In this study, we reported an innovative catalytic process to decompose CO2 in an oxygen-permeation membrane reactor packed with a mixed-conducting oxide supported noble metal catalyst, or Pd/SrCo0.4Fe0.5Zr0.1O3-delta (Pd/ SCFZ), which is of high activity in the decomposition of CO2 into CO and O2. Pd/SCFZ catalyst was prepared by incipient wetness impregnation of the SCFZ powders with an aqueous solution of PdCl2, and the CO2 sorption/desorption property was examined by in situ Fourier transform infrared spectroscopy and temperature-programmed desorption-mass spectrometry technologies. It was shown that there appeared a typical of bridged carbonyls (Pd-CO) on the surface of the Pd/SCFZ catalyst formed after CO2 decomposition. Both CO2 and CO could be detected in the species desorbed from Pd/SCFZ catalyst, which implied that the Pd/SCFZ catalyst could effectively activate the CO2 molecule. During the catalytic process, furthermore, the activity of the Pd/SCFZ catalyst can self-regenerate by removing the produced lattice oxygen through the dense oxygen-permeable ceramic membrane. At 900 degrees C, this catalytic process attains 100% of CO formation selectivity at 15.8% of CO2 conversions.", "gemini": "Phản ứng phân hủy nhiệt CO2 thành CO và O2 là một con đường tiềm năng để tiêu thụ và sử dụng CO2. Tuy nhiên, phản ứng này bị hạn chế bởi cả cân bằng nhiệt động lực học và rào cản động học. Trong nghiên cứu này, chúng tôi đã báo cáo một quy trình xúc tác sáng tạo để phân hủy CO2 trong lò phản ứng màng thấm oxy được đóng gói bằng chất xúc tác kim loại quý được hỗ trợ bởi oxit dẫn hỗn hợp hoặc Pd/SrCo0.4Fe0.5Zr0.1O3-delta (Pd/SCFZ), có hoạt tính cao trong quá trình phân hủy CO2 thành CO và O2. Chất xúc tác Pd/SCFZ được chế tạo bằng cách tẩm ướt ban đầu bột SCFZ bằng dung dịch nước của PdCl2 và tính chất hấp phụ/giải hấp CO2 được kiểm tra bằng công nghệ quang phổ hồng ngoại biến đổi Fourier tại chỗ và công nghệ quang phổ khối nhiệt độ lập trình-giải hấp. Kết quả cho thấy xuất hiện một loại cacbonyl nối cầu (Pd-CO) điển hình trên bề mặt chất xúc tác Pd/SCFZ hình thành sau quá trình phân hủy CO2. Cả CO2 và CO đều có thể được phát hiện trong các loài giải hấp từ chất xúc tác Pd/SCFZ, điều này ngụ ý rằng chất xúc tác Pd/SCFZ có thể kích hoạt hiệu quả phân tử CO2. Hơn nữa, trong quá trình xúc tác, hoạt tính của chất xúc tác Pd/SCFZ có thể tự tái tạo bằng cách loại bỏ oxy mạng lưới được tạo ra thông qua màng gốm thấm oxy dày đặc. Ở 900 độ C, quá trình xúc tác này đạt được 100% độ chọn tạo thành CO ở 15,8% chuyển đổi CO2."} +{"idx": 1970042, "en": "The manufacturing of modern semiconductor devices involves a complex set of nanoscale fabrication processes that are energy and resource intensive, and generate significant waste. It is important to understand and reduce the environmental impacts of semiconductor manufacturing because these devices are ubiquitous components in electronics. Furthermore, the fabrication processes used in the semiconductor industry are finding increasing application in other products, such as microelectromechanical systems (MEMS), flat panel displays, and photovoltaics. In this work we develop a library of typical gate-to-gate materials and energy requirements, as well as emissions associated with a complete set of fabrication process models used in manufacturing a modern microprocessor. In addition, we evaluate upstream energy requirements associated with chemicals and materials using both existing process life cycle assessment (LCA) databases and an economic input-output (EIO) model. The result is a comprehensive data set and methodology that may be used to estimate and improve the environmental performance of a broad range of electronics and other emerging applications that involve nano and micro fabrication.", "gemini": "Việc sản xuất các thiết bị bán dẫn hiện đại liên quan đến một tập hợp phức tạp các quy trình chế tạo ở quy mô nano, đòi hỏi nhiều năng lượng và tài nguyên, đồng thời tạo ra nhiều chất thải đáng kể. Điều quan trọng là phải hiểu và giảm tác động môi trường của quá trình sản xuất chất bán dẫn vì những thiết bị này là thành phần phổ biến trong các thiết bị điện tử. Hơn nữa, các quy trình chế tạo được sử dụng trong ngành công nghiệp bán dẫn đang ngày càng được ứng dụng trong các sản phẩm khác, chẳng hạn như hệ thống vi cơ điện tử (MEMS), màn hình phẳng và quang điện. Trong công trình này, chúng tôi phát triển một thư viện về các vật liệu cổng-cổng thông thường và các yêu cầu về năng lượng, cũng như các khí thải liên quan đến một tập hợp hoàn chỉnh các mô hình quy trình chế tạo được sử dụng trong sản xuất vi xử lý hiện đại. Ngoài ra, chúng tôi đánh giá các yêu cầu năng lượng đầu vào liên quan đến hóa chất và vật liệu bằng cách sử dụng cả cơ sở dữ liệu đánh giá vòng đời quy trình (LCA) hiện có và mô hình đầu vào-đầu ra (EIO) về kinh tế. Kết quả là một tập dữ liệu và phương pháp toàn diện có thể được sử dụng để ước tính và cải thiện hiệu suất môi trường của nhiều loại thiết bị điện tử và các ứng dụng mới nổi khác liên quan đến chế tạo nano và vi mô."} +{"idx": 1970043, "en": "Species sensitivity distributions (SSD) require a large number of measured toxicity values to define a hazard level protective of multiple species. This investigation comprehensively evaluated the accuracy of SSDs generated from toxicity values predicted from interspecies correlation estimation (ICE) models. ICE models are log-log correlations of multiple chemical toxicity values for a pair of species that allow the toxicity of multiple species to be predicted from a single measured acute toxicity value for a surrogate species. ICE SSDs were generated using four surrogate species (fathead minnow, Pimephales promelas; rainbow trout, Oncorhynchus mykiss; sheepshead minnow, Cyprinodon varigatus; and water flea, Daphnia magna). ICE-based hazard concentrations (HC5s) from the 5th percentile of the log-logistic distribution of toxicity values were compared to HC5s determined from the acute toxicity of 55 chemicals from the United States Environmental Protection Agency Ambient Water Quality Criteria (AWQC). Measured fish and invertebrate acute toxicity data and HC5s from the AWQC data sets were compared to ICE-based HC5s. Surrogate species choice was found to be an important consideration in developing predictive HC5s. These results illustrated that fish predict fish betterthan invertebrates and D. magna predicted invertebrates better than most fish. For example, a mixed model of predicted fish and invertebrates from fathead minnow and D. magna as surrogate species provided predictive relationships with an average factor of 3.0 (+/- 6.7) over 7 orders of toxic magnitude and several chemical classes (HC5(predicted)/HC5(measured)). The application of ICE models is recommended as a valid approach for generating SSDs and hazard concentrations for chemicals with limited toxicity data.", "gemini": "Phân bố độ nhạy của loài (SSD) đòi hỏi một số lượng lớn các giá trị độc tính được đo để xác định mức độ nguy hại bảo vệ nhiều loài. Cuộc điều tra này đã đánh giá toàn diện độ chính xác của SSD được tạo ra từ các giá trị độc tính được dự đoán từ các mô hình ước tính tương quan giữa các loài (ICE). Các mô hình ICE là các tương quan log-log của nhiều giá trị độc tính hóa học đối với một cặp loài cho phép dự đoán độc tính của nhiều loài từ một giá trị độc tính cấp tính được đo đối với một loài thay thế. SSD của ICE được tạo ra bằng cách sử dụng bốn loài thay thế (cá tuế đầu bẹt, Pimephales promelas; cá hồi cầu vồng, Oncorhynchus mykiss; cá tuế đầu đen, Cyprinodon varigatus; và bọ nước, Daphnia magna). Nồng độ nguy hiểm dựa trên ICE (HC5) từ phần trăm thứ 5 của phân phối log-logistic của các giá trị độc tính được so sánh với HC5 được xác định từ độc tính cấp tính của 55 hóa chất từ Tiêu chí chất lượng nước xung quanh của Cơ quan bảo vệ môi trường Hoa Kỳ (AWQC). Dữ liệu độc tính cấp tính của cá và động vật không xương sống được đo và HC5 từ các tập dữ liệu của AWQC được so sánh với HC5 dựa trên ICE. Lựa chọn loài thay thế được phát hiện là một cân nhắc quan trọng trong việc phát triển HC5 dự đoán. Những kết quả này minh họa rằng cá dự đoán cá tốt hơn động vật không xương sống và D. magna dự đoán động vật không xương sống tốt hơn hầu hết các loài cá. Ví dụ: một mô hình hỗn hợp về cá và động vật không xương sống được dự đoán từ cá tuế đầu bẹt và D. magna làm loài thay thế cung cấp các mối quan hệ dự đoán với hệ số trung bình là 3,0 (+/- 6,7) trên 7 bậc độ lớn độc hại và một số lớp hóa chất (HC5(dự đoán)/HC5(đo)). Việc áp dụng các mô hình ICE được khuyến nghị là một cách tiếp cận hợp lệ để tạo ra SSD và nồng độ nguy hiểm cho các hóa chất có dữ liệu độc tính hạn chế."} +{"idx": 1970044, "en": "The electrically conductive adhesives (ECA) are on the verge of a breakthrough as reliable interconnection materials for electronic components. As the ban of lead (Pb) in the electronics industry becomes a reality, the ECA's could be attractive overall alternatives to high melting point (HMP) Pb-based solder pastes. Environmental life cycle assessment (LCA) was used to estimate trade-offs between the energy use and the potential toxicity of two future types of ECA's and one HMP Pb-based. The probability is around 90% that the overall CO2 emissions from an ECA based on a tin-bismuth alloy are lower than for a silver-epoxy based ECA, whereas the probability is about 80% that the cumulative energy demand would be lower. It is more uncertain whether the tin-bismuth ECA would contribute to less CO2, or consume less energy, than a HMP Pb-based solder paste. Moreover, for the impact categories contributing to the life-cycle impact assessment method based on end point modeling (LIME) damage category of human health, the tin-bismuth ECA shows a 25 times lower score, and a silver-epoxy based ECA shows an 11 times lower score than the HMP Pb-based solder paste. In order to save resources and decrease CO2 emissions it is recommended to increase the collection and recycling of printed board assemblies using silver-epoxy based ECA.", "gemini": "Các chất kết dính dẫn điện (ECA) đang trên bờ vực của một bước đột phá với tư cách là vật liệu kết nối đáng tin cậy cho các thành phần điện tử. Khi lệnh cấm chì (Pb) trong ngành công nghiệp điện tử trở thành hiện thực, ECA có thể là những lựa chọn thay thế toàn diện hấp dẫn cho kem hàn gốc chì (HMP) có điểm nóng chảy cao. Đánh giá vòng đời môi trường (LCA) đã được sử dụng để ước tính sự đánh đổi giữa việc sử dụng năng lượng và độc tính tiềm ẩn của hai loại ECA trong tương lai và một loại HMP gốc chì. Xác suất khoảng 90% là tổng lượng phát thải CO2 từ ECA dựa trên hợp kim thiếc-bismuth thấp hơn so với ECA dựa trên bạc-epoxy, trong khi xác suất khoảng 80% là nhu cầu năng lượng tích lũy sẽ thấp hơn. Không chắc chắn hơn liệu ECA thiếc-bismuth sẽ góp phần giảm CO2 hay tiêu thụ ít năng lượng hơn so với kem hàn gốc chì HMP. Hơn nữa, đối với các loại tác động góp phần vào phương pháp đánh giá tác động vòng đời dựa trên danh mục thiệt hại mô hình điểm cuối (LIME) về sức khỏe con người, ECA thiếc-bismuth cho thấy điểm số thấp hơn 25 lần và ECA dựa trên bạc-epoxy cho thấy điểm số thấp hơn 11 lần so với kem hàn gốc chì HMP. Để tiết kiệm tài nguyên và giảm phát thải CO2, nên tăng cường thu gom và tái chế các cụm bảng mạch in sử dụng ECA dựa trên bạc-epoxy."} +{"idx": 1970045, "en": "Carbon nanotubes comprise a class of nanomaterials having demonstrated promise for broad ranges of potential applications. Because of difficulties associated with quantifying these materials in environmental media, however, their behaviors therein and associated potential risks are yet largely unknown. To address this problem, a modified chemical vapor deposition process employing carbon-14 labeled methane was used to synthesize single- and multi-walled carbon nanotubes. The labeled nanotubes and a representative polynuclear hydrocarbon, pyrene, were then individually spiked to identical soil samples. The uptake and depuration behaviors of the spiked materials by the earthworm Eisenia foetida, a potential entry point to terrestrial food chains, were then assessed. Bioaccumulation factors determined for the nanotubes were almost 2 orders of magnitude smaller than those measured for pyrene, indicating that purified carbon nanotubes, unlike pyrene, are neither readily absorbed into organism tissues nor manifest equilibrium partitioning thereto.", "gemini": "Các ống nano carbon bao gồm một lớp vật liệu nano đã chứng minh được triển vọng cho nhiều ứng dụng tiềm năng. Tuy nhiên, do những khó khăn liên quan đến việc định lượng các vật liệu này trong môi trường, hành vi của chúng và các rủi ro tiềm ẩn liên quan vẫn chưa được biết đến nhiều. Để giải quyết vấn đề này, một quy trình lắng đọng hơi hóa học đã được sửa đổi sử dụng metan đánh dấu carbon-14 đã được sử dụng để tổng hợp các ống nano carbon thành một và nhiều lớp. Sau đó, các ống nano được đánh dấu và một hydrocarbon polynuclear đại diện, pyren, được thêm riêng vào các mẫu đất giống hệt nhau. Sau đó, hành vi hấp thụ và thanh lọc các vật liệu được thêm vào của giun đất Eisenia foetida, một điểm vào tiềm năng đối với chuỗi thức ăn trên cạn, đã được đánh giá. Các yếu tố tích lũy sinh học được xác định đối với các ống nano nhỏ hơn gần 2 bậc độ lớn so với các yếu tố được đo đối với pyren, cho thấy rằng các ống nano carbon tinh khiết, không giống như pyren, không dễ dàng hấp thụ vào mô cơ thể hoặc biểu hiện phân chia cân bằng đối với chúng."} +{"idx": 1970046, "en": "Perfluorinated compounds (PFCs), notably perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA), have been reported in human blood. Furthermore, the occurrence of PFCs in the blood of newborn babies, coupled with the need to study the potential association of PFC exposure with birth outcomes in neonates, suggests the need for determining the sources and magnitude of exposure in infants. In this study, nine PFCs were measured in 45 human breast milk samples collected in 2004 from Massachusetts, U.S.A. PFOS and PFOA were the predominant PFCs found at mean concentrations of 131 and 43.8 pg/mL, respectively. Comparison of the ratio of PFOS to PFOA in human milk with the ratios published for human serum from the U.S. female population suggested preferential partitioning of PFOA to milk. Concentrations of PFOA were significantly higher in the milk of mothers nursing for the first time (n = 34) than in the milk of mothers who have previously nursed (n = 8). Based on the estimated body weight and milk intake, the average and highest daily intakes of total PFCs by infants were 23.5 and 87.1 ng/kg bw, respectively. We found that the daily ingestion rates of PFOS and PFOA did not exceed the tolerable daily intake recommended by the U.K. Food Standards Agency. This is the first study to measure the occurrence of PFCs in human milk from the U.S.A.", "gemini": "Các hợp chất perfluorinat (PFC), đáng chú ý là perfluorooctanesulfonat (PFOS) và axit perfluorooctanoic (PFOA), đã được báo cáo có trong máu người. Hơn nữa, sự xuất hiện của PFC trong máu của trẻ sơ sinh, cùng với nhu cầu nghiên cứu mối liên hệ tiềm ẩn giữa phơi nhiễm PFC với kết quả sinh ở trẻ sơ sinh, cho thấy cần phải xác định các nguồn và mức độ phơi nhiễm ở trẻ sơ sinh. Trong nghiên cứu này, chín PFC đã được đo trong 45 mẫu sữa mẹ của người được thu thập vào năm 2004 từ Massachusetts, Hoa Kỳ. PFOS và PFOA là những PFC chiếm ưu thế với nồng độ trung bình lần lượt là 131 và 43,8 pg/mL. So sánh tỷ lệ PFOS với PFOA trong sữa mẹ với các tỷ lệ được công bố đối với huyết thanh của phụ nữ Hoa Kỳ cho thấy sự phân chia ưu tiên của PFOA vào sữa. Nồng độ PFOA cao hơn đáng kể trong sữa của những bà mẹ cho con bú lần đầu (n = 34) so với sữa của những bà mẹ đã cho con bú trước đó (n = 8). Dựa trên trọng lượng cơ thể ước tính và lượng sữa nạp vào, lượng PFC tổng hợp trung bình và cao nhất hàng ngày của trẻ sơ sinh lần lượt là 23,5 và 87,1 ng/kg bw. Chúng tôi thấy rằng tỷ lệ hấp thụ hàng ngày của PFOS và PFOA không vượt quá lượng hấp thụ hàng ngày được khuyến nghị của Cơ quan Tiêu chuẩn Thực phẩm Vương quốc Anh. Đây là nghiên cứu đầu tiên đo lường sự xuất hiện của PFC trong sữa mẹ từ Hoa Kỳ."} +{"idx": 1970047, "en": "The short-term (3 months) dynamics of bioavailable copper (Cu) species was determined in soils amended with various amounts of manure and Cu. Bioavailable Cu species were operationally defined as those species that were able to induce gene expression in a Cu-specific Pseudomonas fluorescens biosensor. Biosensor measurements were backed by analysis of total Cu in soil and of total Cu and free Cu2+ ion activity in solution. Cu bioavailability relative to the total Cu concentration increased dramatically with increasing Cu loading of manure and with increasing manure amendment to soil. In both cases, the immediate increase in bioavailability could be explained in part by increased Cu concentration in solution and in part by an increased bioavailability of dissolved Cu species. In contrast to Cu bioavailability, Cu2+ ion activity decreased progressively with increasing manure loading. Cu bioavailability declined rapidly during the weeks after manure amendment concomitant with a marked slow-down of C mineralization indicating a shift from initially bioavailable Cu-dissolved organic matter (Cu-DOM) complexes to nonavailable Cu-DOM complexes over time. Our data do not support the conventional view of metal bioavailability being primarily related to the free metal ion activity and strongly suggest differential bioavailability of Cu-DOM complexes in manure-amended soils.", "gemini": "Động lực ngắn hạn (3 tháng) của các loài đồng (Cu) có thể hấp thụ sinh học đã được xác định trong đất được bổ sung lượng phân và Cu khác nhau. Các loài Cu có thể hấp thụ sinh học được xác định theo hoạt động là các loài có khả năng gây ra biểu hiện gen trong một cảm biến sinh học Pseudomonas fluorescens đặc hiệu Cu. Các phép đo cảm biến sinh học được hỗ trợ bằng phân tích tổng lượng Cu trong đất và tổng lượng Cu và hoạt độ ion Cu2+ tự do trong dung dịch. Khả dụng sinh học của Cu so với nồng độ Cu tổng thể tăng đáng kể khi tăng tải Cu của phân và tăng lượng phân bổ sung vào đất. Trong cả hai trường hợp, sự gia tăng khả dụng sinh học ngay lập tức có thể được giải thích một phần là do tăng nồng độ Cu trong dung dịch và một phần là do tăng khả dụng sinh học của các loài Cu hòa tan. Ngược lại với khả dụng sinh học của Cu, hoạt độ ion Cu2+ giảm dần khi tăng tải phân. Khả dụng sinh học của Cu giảm nhanh chóng trong những tuần sau khi bổ sung phân cùng với sự chậm lại rõ rệt của quá trình khoáng hóa C, cho thấy sự thay đổi từ các phức hợp vật chất hữu cơ hòa tan Cu (Cu-DOM) có thể hấp thụ sinh học ban đầu sang các phức hợp Cu-DOM không có sẵn theo thời gian. Dữ liệu của chúng tôi không ủng hộ quan điểm thông thường cho rằng khả dụng sinh học của kim loại chủ yếu liên quan đến hoạt độ ion kim loại tự do và cho thấy rõ ràng khả dụng sinh học khác biệt của các phức hợp Cu-DOM trong đất được bổ sung phân."} +{"idx": 1970048, "en": "The effects of accumulated Se on the reproductive success and larval development of cutthroat trout (Oncorhynchus clarki lewis,) collected from a site of active coal mining in British Columbia were assessed. Eggs from 12 fish from an exposed site (Clode Pond) and 16 from a reference site (O'Rourke Lake) were field-collected and reared in the laboratory. Egg Se concentrations ranged from 12.3 to 16.7 and 11.8 to 140.0 microg/g dry weight (dw) from fish collected at the reference and exposed sites, respectively. Other studies, including those with this species, have not shown Se to affect egg viability; however, in the present study, eggs with Se concentrations > 86.3 microg/g dw were not successfully fertilized or were nonviable at fertilization, while eggs with concentrations > 46.8 and < 75.4 microg/g dw were fertilized (96% reached the eyed stage) but did not produce viable fry. A significant positive relationship between egg Se concentration and alevin mortality was observed. Deformities were analyzed in surviving fry which developed from eggs with Se concentrations between 11.8 and 20.6 microg/g dw. No relationship between Se concentration in eggs and deformities or edema was found in this range, suggesting that the no-effect threshold for deformity is > 20.6 microg/g dw. The present data, in conjunction with the data from several other studies in temperate fish, suggest that current Se thresholds are conservative for cold-water fish.", "gemini": "Đã đánh giá tác động của Se tích lũy đối với sự sinh sản thành công và sự phát triển ấu trùng của cá hồi cắt cổ họng (Oncorhynchus clarki lewis) thu thập từ một địa điểm khai thác than đang hoạt động ở British Columbia. Trứng từ 12 con cá từ một địa điểm tiếp xúc (Clode Pond) và 16 con từ một địa điểm tham chiếu (Hồ O'Rourke) đã được thu thập tại thực địa và nuôi trong phòng thí nghiệm. Nồng độ Se trong trứng dao động từ 12,3 đến 16,7 và 11,8 đến 140,0 microg/g trọng lượng khô (dw) từ cá thu được ở địa điểm tham chiếu và địa điểm tiếp xúc. Các nghiên cứu khác, bao gồm cả những nghiên cứu về loài này, đã không chỉ ra rằng Se ảnh hưởng đến khả năng sống của trứng; tuy nhiên, trong nghiên cứu hiện tại, những quả trứng có nồng độ Se> 86,3 microg/g dw không được thụ tinh thành công hoặc không có khả năng sống khi thụ tinh, trong khi những quả trứng có nồng độ> 46,8 và <75,4 microg/g dw đã được thụ tinh (96% đạt giai đoạn có mắt) nhưng không tạo ra cá bột khả thi. Một mối quan hệ tích cực đáng kể giữa nồng độ Se trong trứng và tỷ lệ tử vong của cá bột đã được quan sát thấy. Những dị tật đã được phân tích ở cá bột sống sót phát triển từ những quả trứng có nồng độ Se từ 11,8 đến 20,6 microg/g dw. Không tìm thấy mối quan hệ nào giữa nồng độ Se trong trứng và dị tật hoặc phù nề trong phạm vi này, điều này cho thấy ngưỡng không có tác dụng đối với dị tật là> 20,6 microg/g dw. Dữ liệu hiện tại, kết hợp với dữ liệu từ một số nghiên cứu khác về cá ôn đới, cho thấy rằng ngưỡng Se hiện tại là bảo thủ đối với cá nước lạnh."} +{"idx": 1970049, "en": "An oral health promotion programme and study are currently being carried out in 9 nursery schools with children at high risk for cavities located in the deprived neighbourhoods of the city of Clermont-Ferrand (France). The programme's objective is to promote the improvement of tooth brushing habits (2 times/day) and general oral hygiene associated with the use of fluoridated toothpastes. Dental care and dental hygiene visits are also encouraged. In 2005-2006 the programme was implemented in 4 schools; another group of 5 schools constituted a control group. After one year, an evaluation of the process, the programme's effectiveness and short-term outcomes was conducted. The majority of the parents, teachers and children participated in the program but their involvement was limited, with active participation being difficult to achieve. The programme was positive in terms of its prevention component and objective. A significant improvement in children's oral hygiene habits was obtained in the programme group as compared to the control group. However, the care and treatment needs of the children in both groups were not covered, given that one child out of four remained with untreated lesions at the end of the year. These results suggest that here are many barriers that prevent children's access to care which need to be addressed.", "gemini": "Một chương trình và nghiên cứu thúc đẩy sức khỏe răng miệng hiện đang được thực hiện tại 9 trường mẫu giáo với trẻ em có nguy cơ sâu răng cao nằm ở các khu dân cư nghèo của thành phố Clermont-Ferrand (Pháp). Mục tiêu của chương trình là thúc đẩy việc cải thiện thói quen đánh răng (2 lần/ngày) và vệ sinh răng miệng nói chung kết hợp với việc sử dụng kem đánh răng có chứa fluoride. Các chuyến thăm chăm sóc nha khoa và vệ sinh răng miệng cũng được khuyến khích. Trong năm 2005-2006, chương trình đã được triển khai tại 4 trường; một nhóm 5 trường khác được lập thành nhóm đối chứng. Sau một năm, một đánh giá về quá trình, hiệu quả của chương trình và kết quả trong thời gian ngắn đã được tiến hành. Phần lớn phụ huynh, giáo viên và trẻ em đã tham gia chương trình nhưng sự tham gia của họ còn hạn chế, khó đạt được sự tham gia tích cực. Chương trình mang tính tích cực về mặt phòng ngừa và mục tiêu của nó. Một sự cải thiện đáng kể về thói quen vệ sinh răng miệng của trẻ em đã đạt được trong nhóm chương trình so với nhóm đối chứng. Tuy nhiên, nhu cầu chăm sóc và điều trị của trẻ em trong cả hai nhóm vẫn chưa được đáp ứng, vì một trong bốn trẻ vẫn còn các tổn thương chưa được điều trị vào cuối năm. Những kết quả này cho thấy rằng có nhiều rào cản ngăn cản trẻ em tiếp cận dịch vụ chăm sóc cần được giải quyết."} +{"idx": 1970050, "en": "The aim of this study was to explore and document the perceptions of health professionals regarding parental participation in the fight against child malaria, specifically with regard to fever management, in Benin (West Africa). The findings indicate that community participation in Malaria control in general, and in the case of children in particular, is essential due to the contributing factors surrounding the disease. This form of participation is different from the one recommended and practiced within the structure of the National Health system. Deep-seated, thorough reform of health care program planning is necessary in order to contribute to the improvement of the population's participation in this process. The concept of health promotion was used to frame and shed light on the analysis of the proposed recommendations. Parents' participation, one that integrates their perceptions and depictions of child fever, is not currently a reality in Benin; the study revealed that the participants seek out and request the development of skills necessary that could enable them to better contribute to the fight against malaria.", "gemini": "Mục đích của nghiên cứu này là khám phá và ghi lại nhận thức của các chuyên gia y tế về sự tham gia của cha mẹ trong cuộc chiến chống lại bệnh sốt rét ở trẻ em, đặc biệt là về cách kiểm soát sốt, tại Benin (Tây Phi). Các phát hiện chỉ ra rằng sự tham gia của cộng đồng vào việc kiểm soát bệnh sốt rét nói chung, và đối với trẻ em nói riêng, là điều cần thiết do các yếu tố góp phần gây ra căn bệnh này. Hình thức tham gia này khác với hình thức được khuyến nghị và thực hành trong cấu trúc của hệ thống Y tế Quốc gia. Cần cải cách sâu rộng, toàn diện chương trình chăm sóc sức khỏe để góp phần nâng cao sự tham gia của người dân vào quá trình này. Khái niệm thúc đẩy sức khỏe đã được sử dụng để đóng khung và làm sáng tỏ việc phân tích các khuyến nghị được đề xuất. Sự tham gia của cha mẹ, sự tham gia tích hợp nhận thức và mô tả của họ về sốt ở trẻ em, hiện không phải là hiện thực ở Benin; nghiên cứu cho thấy rằng những người tham gia tìm kiếm và yêu cầu phát triển các kỹ năng cần thiết có thể giúp họ đóng góp tốt hơn vào cuộc chiến chống lại bệnh sốt rét."} +{"idx": 1970051, "en": "Mortality rates are an important source of data for French public health observatories. These data are exhaustive, permanent, and available at various geographical levels. Nevertheless, the data are biased to some extent, namely due to the lack of a detailed description of the cause of death on the death certificate, and the causes of death are not systematically recorded when a forensic investigation has been carried out (\"unknown causes\"). This generates in particular an underestimation of suicide mortality in France. We studied these biases in south-eastern France for the five-year period 1995-1999, and the evolution of them between 1988-1992 and 1995-1999. In comparison with the national level statistics, deaths with an imprecise cause were more frequently recorded in south-eastern France (+30%) as was for those whose cause was unknown or non-declared (+100% in the most eastern part of the area). Thus, indicators of suicide mortality should be interpreted with caution, and compared and considered in relationship to the rate of deaths linked to an \"unknown\" cause. The article includes a discussion of the extent to which the transfer of data from institutes of forensic medicine to the French epidemiological centre of the medical causes of death (Inserm CepiDC), and physicians' practices play a role.", "gemini": "Tỷ lệ tử vong là một nguồn dữ liệu quan trọng đối với các đài quan sát sức khỏe cộng đồng của Pháp. Những dữ liệu này là toàn diện, lâu dài và có sẵn ở nhiều cấp độ địa lý khác nhau. Tuy nhiên, dữ liệu có một số thiên lệch, cụ thể là do thiếu mô tả chi tiết về nguyên nhân tử vong trên giấy chứng tử và nguyên nhân tử vong không được ghi chép một cách có hệ thống khi đã tiến hành điều tra pháp y (\"nguyên nhân không xác định\"). Điều này đặc biệt tạo ra sự đánh giá thấp tỷ lệ tử vong do tự tử ở Pháp. Chúng tôi đã nghiên cứu những sự thiên lệch này ở đông nam nước Pháp trong giai đoạn năm năm 1995-1999 và sự tiến triển của chúng trong giai đoạn 1988-1992 và 1995-1999. So với số liệu thống kê ở cấp quốc gia, số ca tử vong có nguyên nhân không rõ ràng được ghi nhận nhiều hơn ở đông nam nước Pháp (+ 30%) cũng như những ca tử vong có nguyên nhân không rõ hoặc không được kê khai (+ 100% ở phần đông nhất của khu vực). Do đó, các chỉ số về tỷ lệ tử vong do tự tử cần được giải thích một cách thận trọng, so sánh và xem xét liên quan đến tỷ lệ tử vong có nguyên nhân \"không xác định\". Bài báo có thảo luận về mức độ chuyển dữ liệu từ các viện y học pháp y sang trung tâm dịch tễ học Pháp về các nguyên nhân tử vong do y tế (Inserm CepiDC) và vai trò của các bác sĩ."} +{"idx": 1970052, "en": "Formative supervision is a quality and performance tool based on evaluation and adult training techniques. The 2004 cholera outbreak in Douala (Cameroon) presented a critical problem in terms of quality of care; formative supervision emerged as the choice of instrument developed as a key response and solution. After a chronological qualitative description of how the supervision team and system were constituted, established and organized, the results are presented: strengthening infrastructure, equipment and organisation; improving the quality of care, hygiene, communication, and management. The system requires capacity building for sustainability in order to also be eventually extended to other health districts and other health sector activities, on the condition that the necessary resources can be mobilized.", "gemini": "Giám sát hình thành là một công cụ về chất lượng và hiệu suất dựa trên các kỹ thuật đánh giá và đào tạo người lớn. Đợt bùng phát dịch tả năm 2004 tại Douala (Cameroon) đã nêu ra một vấn đề nghiêm trọng về chất lượng chăm sóc; giám sát hình thành nổi lên như sự lựa chọn của công cụ được phát triển như một giải pháp và phản ứng chính. Sau khi mô tả định tính theo trình tự thời gian về cách thành lập, thiết lập và tổ chức nhóm và hệ thống giám sát, các kết quả được trình bày: tăng cường cơ sở hạ tầng, trang thiết bị và tổ chức; nâng cao chất lượng chăm sóc, vệ sinh, giao tiếp và quản lý. Hệ thống này đòi hỏi phải xây dựng năng lực để duy trì tính bền vững để cuối cùng cũng có thể mở rộng sang các quận y tế khác và các hoạt động khác của ngành y tế, với điều kiện có thể huy động được các nguồn lực cần thiết."} +{"idx": 1970053, "en": "This experiment evaluated the development and implementation of 5 pilot health education activities using a comprehensive and population-based approach in general medicine practice and in pharmacies. These interventions involved 185 patients, and they were developed and carried out by 35 general practitioners and pharmacists in 5 sites in France between 2001 and 2004. Health professionals often demonstrated and argued for a comprehensive approach to health: positive vision of health, integration of different aspects of health, and personalized, tailored efforts. Pregnant women, adolescents, and elderly people have certain specificities that must be taken into account in a population-based approach. The activities were based on simple techniques using interview guidelines and informational documents to aid and support both expressiveness and the professional-patient relationship.", "gemini": "Thí nghiệm này đã đánh giá sự phát triển và triển khai của 5 hoạt động giáo dục sức khỏe thí điểm bằng cách sử dụng một cách tiếp cận toàn diện và dựa trên dân số trong thực hành y học nói chung và trong các hiệu thuốc. Các can thiệp này liên quan đến 185 bệnh nhân và được 35 bác sĩ đa khoa và dược sĩ phát triển và thực hiện tại 5 địa điểm ở Pháp từ năm 2001 đến năm 2004. Các chuyên gia y tế thường trình bày và tranh luận về một cách tiếp cận toàn diện đối với sức khỏe: quan điểm tích cực về sức khỏe, tích hợp các khía cạnh khác nhau của sức khỏe và các nỗ lực được cá nhân hóa, phù hợp. Phụ nữ mang thai, thanh thiếu niên và người cao tuổi có một số đặc điểm cụ thể cần được tính đến trong cách tiếp cận dựa trên dân số. Các hoạt động dựa trên các kỹ thuật đơn giản sử dụng hướng dẫn phỏng vấn và tài liệu thông tin để hỗ trợ và hỗ trợ cả khả năng diễn đạt và mối quan hệ bác sĩ-bệnh nhân."} +{"idx": 1970054, "en": "The article aims to present critical elements to support inter-cultural reflection based upon a sociological reference framework. This necessitates engagement in a process of exchange that takes local context into account. This approach, using a dynamic relationship of interaction, provides opportunity for re-balancing, adjustment, and monitoring and regulation of the implementation of health promotion programmes. Finally, it also allows for better supervision and driving change for action on the social determinants of health as well as the step by step development of a more equitable ethical core group in health. This approach is based on the validity and relevance of the skills transfer and on the conditions of its implementation.", "gemini": "Bài viết này nhằm trình bày các yếu tố quan trọng để hỗ trợ sự phản ánh giữa các nền văn hóa dựa trên một khuôn khổ tham chiếu về mặt xã hội học. Điều này đòi hỏi phải tham gia vào một quá trình trao đổi có tính đến bối cảnh địa phương. Phương pháp tiếp cận này, sử dụng mối quan hệ tương tác động, tạo cơ hội để cân bằng lại, điều chỉnh, theo dõi và điều chỉnh việc thực hiện các chương trình thúc đẩy sức khỏe. Cuối cùng, phương pháp này cũng cho phép giám sát và thúc đẩy thay đổi tốt hơn cho hành động đối với các yếu tố xã hội quyết định sức khỏe cũng như sự phát triển từng bước của một nhóm cốt lõi về đạo đức công bằng hơn trong lĩnh vực y tế. Phương pháp tiếp cận này dựa trên tính hợp lệ và sự phù hợp của việc chuyển giao kỹ năng và các điều kiện thực hiện."} +{"idx": 1970055, "en": "The third generation of the regional healthcare organization plan (Sros III) proposes to develop the organisation of healthcare and its management according to evolution of its activities and the populations concerned. At the time of a strategic analysis of SROS III (what we refer to as its perinatal period), the question is whether promoters can move from an approach based on accessibility (egalitarian equity) to a needs-based approach (differential equity), which although more complicated in to apply and implement, was found be much better adapted to healthcare users. The research is derived from an analysis of documents from November 2004 to November 2006. A university public hospital developed the data which supported a proposal to shift from level I to level II. This proposition was retained in the territory's medical plan authorized by the regional health authorities. Health professionals and the architects of the healthcare plan have the capacity to new organizations responsible for taking into account the activities and healthcare needs of the population in order to initiate and establish differential equity.", "gemini": "Thế hệ thứ ba của kế hoạch tổ chức chăm sóc sức khỏe khu vực (Sros III) đề xuất phát triển tổ chức chăm sóc sức khỏe và quản lý của tổ chức này theo sự phát triển của các hoạt động và đối tượng dân số liên quan. Vào thời điểm phân tích chiến lược của SROS III (mà chúng tôi gọi là giai đoạn chu sinh của kế hoạch này), câu hỏi đặt ra là liệu những người thúc đẩy có thể chuyển từ cách tiếp cận dựa trên khả năng tiếp cận (công bằng bình đẳng) sang cách tiếp cận dựa trên nhu cầu (công bằng khác biệt) hay không, mặc dù cách tiếp cận này phức tạp hơn trong việc áp dụng và triển khai, nhưng được thấy là phù hợp hơn nhiều với những người sử dụng dịch vụ chăm sóc sức khỏe. Nghiên cứu được bắt nguồn từ việc phân tích các tài liệu từ tháng 11 năm 2004 đến tháng 11 năm 2006. Một bệnh viện công của trường đại học đã phát triển dữ liệu hỗ trợ đề xuất chuyển từ cấp độ I sang cấp độ II. Đề xuất này được giữ lại trong kế hoạch y tế của lãnh thổ được các cơ quan y tế khu vực cho phép. Các chuyên gia y tế và những người xây dựng kế hoạch chăm sóc sức khỏe có năng lực đối với các tổ chức mới chịu trách nhiệm tính đến các hoạt động và nhu cầu chăm sóc sức khỏe của dân số để khởi xướng và thiết lập công bằng khác biệt."} +{"idx": 1970056, "en": "Acute renal failure is still associated with a high mortality rate among intensive care patients, despite improvements in renal replacement therapies. The available intermittent and continuous renal replacement therapy techniques all have their own advantages and disadvantages. The best time to start renal replacement therapy and various other practical aspects of these techniques still need to be clarified. For these reasons, daily consultation between intensivists and nephrologists is necessary to select the best technique for the ICU patient with acute renal failure, based on the hemodynamic status, biological variables (uremia, coagulation, acid-base balance, electrolytes) and effects on other organ systems (cognitive state, pericarditis, myopathy). In the future, new markers of renal dysfunction may facilitate treatment choices.", "gemini": "Suy thận cấp vẫn liên quan đến tỷ lệ tử vong cao ở những bệnh nhân chăm sóc đặc biệt, mặc dù các liệu pháp thay thế thận đã được cải thiện. Tất cả các kỹ thuật thay thế thận liên tục và ngắt quãng hiện có đều có những ưu điểm và nhược điểm riêng. Thời điểm tốt nhất để bắt đầu liệu pháp thay thế thận và nhiều khía cạnh thực tế khác của các kỹ thuật này vẫn cần được làm rõ. Vì những lý do này, cần phải có sự tham vấn hàng ngày giữa các bác sĩ hồi sức và bác sĩ chuyên khoa thận để lựa chọn kỹ thuật tốt nhất cho bệnh nhân ICU bị suy thận cấp, dựa trên tình trạng huyết động, các biến số sinh học (urê máu, đông máu, cân bằng kiềm toan, chất điện giải) và tác động đến các hệ cơ quan khác (trạng thái nhận thức, viêm màng ngoài tim, bệnh cơ). Trong tương lai, các dấu hiệu mới của rối loạn chức năng thận có thể tạo điều kiện thuận lợi cho việc lựa chọn phương pháp điều trị."} +{"idx": 1970057, "en": "This article summarizes the contribution of the Department of Nephrology, Dialysis, and renal Transplantation to the research in the field of haemodialysis during the last twenty years. The most important contributions are devoted, on the one hand, to the infections and immune defenses of the dialysis patient, on the other hand, to the biocompatibility of the materials used for haemodialysis and to the adverse reactions of the patient to these materials.", "gemini": "Bài báo này tóm tắt những đóng góp của Khoa Thận, Thận nhân tạo và Ghép thận vào công trình nghiên cứu trong lĩnh vực thẩm phân máu trong hai mươi năm qua. Những đóng góp quan trọng nhất tập trung vào một mặt là các bệnh nhiễm trùng và cơ chế miễn dịch của bệnh nhân thẩm phân, mặt khác là khả năng tương hợp sinh học của các vật liệu được sử dụng để thẩm phân máu và các phản ứng có hại của bệnh nhân đối với những vật liệu này."} +{"idx": 1970058, "en": "The World Kidney Day offers a crucial opportunity to the Belgian associations of nephrologists to inform public and medical communities about the global problem of chronic kidney disease (CKD). According to epidemiological data from GNFB/ NBVN registries in our country, cardiovascular diseases and diabetes 2 are responsible for an increasing amount of CKD cases with pejorative evolution to end-stage renal disease requiring cost-effective renal replacement therapies (haemodialysis, peritoneal dialysis or renal transplantation). In these patients, the high level of comorbidities and the late referral to the nephrologists are recognized as enhanced morbidity and mortality factors. These facts underline the need for the nephrologists to stimulate collaboration with general practitioners and specialists from other disciplines in order to set up systematic screening procedures and early follow-up of patients at risk of developing CKD. These procedures should contribute to reduce the progression rate of renal function impairment.", "gemini": "Ngày Thận thế giới mang đến một cơ hội quan trọng cho các hiệp hội bác sĩ chuyên khoa thận của Bỉ để thông tin cho cộng đồng và các cộng đồng y tế về vấn đề toàn cầu về bệnh thận mãn tính (CKD). Theo dữ liệu dịch tễ học từ các sổ đăng ký GNFB/NBVN tại quốc gia chúng tôi, các bệnh tim mạch và bệnh tiểu đường loại 2 gây ra một lượng lớn các trường hợp CKD với diễn biến xấu đến giai đoạn cuối của bệnh thận, đòi hỏi các liệu pháp thay thế thận hiệu quả về mặt chi phí (thẩm phân máu, thẩm phân phúc mạc hoặc ghép thận). Ở những bệnh nhân này, mức độ bệnh đi kèm cao và việc chuyển đến bác sĩ chuyên khoa thận muộn được công nhận là các yếu tố làm tăng tỷ lệ mắc bệnh và tử vong. Những thực tế này nhấn mạnh nhu cầu các bác sĩ chuyên khoa thận phải thúc đẩy sự hợp tác với các bác sĩ đa khoa và các chuyên gia từ các chuyên ngành khác để thiết lập các quy trình sàng lọc có hệ thống và theo dõi sớm những bệnh nhân có nguy cơ mắc CKD. Các quy trình này sẽ góp phần làm giảm tỷ lệ tiến triển của suy giảm chức năng thận."} +{"idx": 1970059, "en": "Since 1965, more than 2000 renal transplantations (including more than 100 living-donor transplantations) have been performed at the University of Brussels. An end-stage renal disease patient candidate to renal transplantation will be therefore followed from his enrolment on the waiting list to the long-term post-transplant period. Improvement in the outcome of renal transplantation is achieved due to better knowledge in many fields of medicine, such as immunology, infectious disease, metabolic diseases (hyperlipemia, diabetes mellitus), pharmacology, use of immunosuppressive regimen, a more adequate cardiovascular prevention and treatment. If the best results were achieved with kidneys from living donors, the graft survival rate at the University of Brussels was nearly 80% for the last period (2000-2006). Unfortunately, renal transplantation cannot cure certain comorbid conditions and even may promote them: infectious diseases, neoplasia, metabolic disorders (e.a diabetes mellitus, hyperlipemia). Many efforts have to be done to develop less toxic and more immune selective therapeutic strategies. Living donation and extension of the pool of cadaveric donors will reduce the length of time spent on the waiting list and will significantly impact on mortality and morbidity after kidney transplantation.", "gemini": "Kể từ năm 1965, hơn 2000 ca ghép thận (bao gồm hơn 100 ca ghép từ người hiến tặng đang sống) đã được thực hiện tại Đại học Brussels. Do đó, một bệnh nhân mắc bệnh thận giai đoạn cuối là ứng cử viên ghép thận sẽ được theo dõi từ khi đăng ký vào danh sách chờ đến giai đoạn dài hạn sau ghép. Việc cải thiện kết quả ghép thận đạt được là nhờ kiến thức tốt hơn trong nhiều lĩnh vực y học, chẳng hạn như miễn dịch học, bệnh truyền nhiễm, bệnh chuyển hóa (tăng lipid máu, đái tháo đường), dược lý học, sử dụng chế độ ức chế miễn dịch, dự phòng và điều trị tim mạch đầy đủ hơn. Nếu đạt được kết quả tốt nhất với thận từ người hiến tặng đang sống, thì tỷ lệ sống sót của ghép tại Đại học Brussels đạt gần 80% trong giai đoạn cuối (2000-2006). Thật không may, ghép thận không thể chữa khỏi một số tình trạng bệnh đi kèm nhất định và thậm chí có thể thúc đẩy chúng: các bệnh truyền nhiễm, tân sinh, các rối loạn chuyển hóa (ví dụ đái tháo đường, tăng lipid máu). Cần phải có nhiều nỗ lực để phát triển các chiến lược điều trị ít độc hại hơn và chọn lọc miễn dịch hơn. Việc hiến tặng khi còn sống và mở rộng nhóm người hiến tặng tử thi sẽ làm giảm thời gian nằm trong danh sách chờ và sẽ tác động đáng kể đến tỷ lệ tử vong và mắc bệnh sau khi ghép thận."} +{"idx": 1970060, "en": "Recent developments in basic and translational immunology open new exciting perspectives for the induction of transplantation tolerance in the clinic. However, a number of hurdles still need to be overcome before immunosuppressive drugs can be safely withdrawn in solid organ transplant recipients. With this background, the European Commission recently launched several initiatives to tackle unmet needs in transplantation medicine. Herein, we focus attention on the RISET project, an ongoing collaborative effort across the European Union aiming at minimization of immunosuppression on the basis of validated biomarkers.", "gemini": "Những tiến bộ gần đây trong miễn dịch học cơ bản và chuyển dịch mở ra những triển vọng thú vị mới cho việc gây ra tình trạng dung nạp ghép trong lâm sàng. Tuy nhiên, vẫn cần phải vượt qua một số rào cản trước khi có thể ngừng thuốc ức chế miễn dịch một cách an toàn ở những người nhận ghép tạng đặc. Với bối cảnh này, Ủy ban Châu Âu gần đây đã đưa ra một số sáng kiến để giải quyết các nhu cầu chưa được đáp ứng trong y học ghép tạng. Ở đây, chúng tôi tập trung sự chú ý vào dự án RISET, một nỗ lực hợp tác đang diễn ra trên khắp Liên minh Châu Âu nhằm mục đích giảm thiểu ức chế miễn dịch trên cơ sở các dấu ấn sinh học đã được xác nhận."} +{"idx": 1970061, "en": "After a short historical background of the Laboratory, the main research topics--renal toxicology, physiopathology of renal interstitial fibrosis and hormonology--are described in the perspective of a partnership between research clinicians and full time scientists. National as well as international scientific collaborations underline the need of combining expertises, stimulating also the training of youngest colleagues to the experimental approach of their future discipline.", "gemini": "Sau một bối cảnh lịch sử ngắn gọn về Phòng thí nghiệm, các chủ đề nghiên cứu chính--độc tính thận, sinh lý bệnh của xơ kẽ mô kẽ thận và nội tiết học--được mô tả theo quan điểm hợp tác giữa các bác sĩ lâm sàng nghiên cứu và các nhà khoa học toàn thời gian. Sự hợp tác khoa học trong nước cũng như quốc tế nhấn mạnh nhu cầu kết hợp chuyên môn, đồng thời thúc đẩy đào tạo những đồng nghiệp trẻ nhất tiếp cận thực nghiệm về chuyên ngành tương lai của họ."} +{"idx": 1970062, "en": "The government has adopted the five outcomes of Every Child Matters as guiding principles for all those caring for and working with children. One of the ways in which efforts are being made to help children achieve good physical health is to encourage them to eat 'five fruit and vegetables a day'. This article sets out the case that practitioners can help children achieve good mental health by encouraging parents and those who care for children to give them at least'five praises a day'. Babies are predisposed from birth to make close social and emotional attachments with their main caregivers, and typically receive generous and loving admiration and appreciation. However, we know that some parents may not understand how infants and toddlers continue to need active nurturing attention, praise and positive messages from those who care for them as they grow. The authors seek to develop their inter-professional campaign to extend the 'five fruit and vegetables a day' maxim to include'five praises a day' for children. Health visitors are uniquely placed to help parents, to explain and encourage the contribution that praise and positive feedback make toward children's general wellbeing and sound mental health.", "gemini": "Chính phủ đã áp dụng năm kết quả của Every Child Matters làm nguyên tắc chỉ đạo cho tất cả những người chăm sóc và làm việc với trẻ em. Một trong những cách mà các nỗ lực đang được thực hiện để giúp trẻ em đạt được sức khỏe thể chất tốt là khuyến khích chúng ăn 'năm loại trái cây và rau mỗi ngày'. Bài viết này nêu rõ trường hợp mà các học viên có thể giúp trẻ em đạt được sức khỏe tinh thần tốt bằng cách khuyến khích cha mẹ và những người chăm sóc trẻ em dành cho chúng ít nhất 'năm lời khen mỗi ngày'. Trẻ sơ sinh có khuynh hướng bẩm sinh tạo ra sự gắn bó xã hội và tình cảm chặt chẽ với những người chăm sóc chính của mình và thường nhận được sự ngưỡng mộ và đánh giá cao một cách hào phóng và yêu thương. Tuy nhiên, chúng ta biết rằng một số phụ huynh có thể không hiểu rằng trẻ sơ sinh và trẻ mới biết đi vẫn tiếp tục cần sự chú ý nuôi dưỡng tích cực, lời khen ngợi và những thông điệp tích cực từ những người chăm sóc chúng khi chúng lớn lên. Các tác giả tìm cách phát triển chiến dịch liên ngành của mình để mở rộng câu châm ngôn 'năm loại trái cây và rau mỗi ngày' bao gồm 'năm lời khen mỗi ngày' dành cho trẻ em. Các nhân viên y tế có vị trí đặc biệt để giúp đỡ cha mẹ, giải thích và khuyến khích sự đóng góp mà lời khen ngợi và phản hồi tích cực mang lại đối với sức khỏe tổng quát và sức khỏe tinh thần tốt của trẻ em."} +{"idx": 1970063, "en": "Influenza is an important cause of morbidity and mortality, particularly in certain high-risk groups and the elderly. However, uptake rates of influenza vaccination by people in high-risk groups have been low. The aim of this study was to explore knowledge and views about the influenza vaccination in adults under 65 years with a long-term condition, in order to identify influences on people's decisions to be immunised and thereby improve uptake rates of the vaccination. Recommendation from a healthcare professional and the perception of being in a high-risk group were major factors in deciding to accept the influenza vaccination. The risk of side effects appeared to play a far smaller part in the decision-making process among the individuals in this study group than in the older age groups studied previously by other researchers.", "gemini": "Cúm là một nguyên nhân quan trọng gây bệnh tật và tử vong, đặc biệt là ở một số nhóm có nguy cơ cao và người cao tuổi. Tuy nhiên, tỷ lệ tiêm phòng cúm ở những người trong nhóm nguy cơ cao vẫn còn thấp. Mục đích của nghiên cứu này là tìm hiểu kiến thức và quan điểm về vắc-xin phòng cúm ở những người lớn dưới 65 tuổi mắc bệnh mãn tính, nhằm xác định những ảnh hưởng đến quyết định tiêm chủng của mọi người và do đó cải thiện tỷ lệ tiêm chủng của vắc-xin. Khuyến cáo từ chuyên gia chăm sóc sức khỏe và nhận thức về việc bản thân thuộc nhóm nguy cơ cao là những yếu tố chính trong việc quyết định chấp nhận tiêm vắc-xin cúm. Rủi ro về tác dụng phụ dường như đóng một phần nhỏ hơn nhiều trong quá trình ra quyết định ở những cá nhân trong nhóm nghiên cứu này so với những nhóm tuổi lớn hơn đã được các nhà nghiên cứu khác nghiên cứu trước đây."} +{"idx": 1970064, "en": "Despite the widespread use of screening for postnatal depression using the Edinburgh Postnatal Depression Scale (EPDS) in England, little is known about the views of those healthcare professionals (HPs) who administer it. The current study aimed to address this, using a qualitative methodology to access the perceptions of HPs toward screening using this tool. A total of 19 semi-structured interviews were conducted in one primary care Trust (PCT). The results were analysed in the context of recommendations made by Unite/CPHVA and the National Screening Committee (NSC). Although training was provided in the PCT, some HPs had screened before being trained. All participants felt that the EPDS was useful, but for some this was only as a way to introduce the subject of postnatal depression. While some thought the tool was good generally, others reported problems with it, particularly in relation to item 10 on self harm. We found large differences in screening practice related to timing, environment and repeat screenings. We have made suggestions to alter practice so that it both meets recommendations made by Unite/CPHVA and the NSC, and ensures women receive the same standard of care across the PCT.", "gemini": "Mặc dù việc sàng lọc trầm cảm sau sinh bằng Thang trầm cảm sau sinh Edinburgh (EPDS) được sử dụng rộng rãi ở Anh, nhưng người ta vẫn biết rất ít về quan điểm của những chuyên gia chăm sóc sức khỏe (HP) thực hiện sàng lọc này. Nghiên cứu hiện tại nhằm giải quyết vấn đề này, sử dụng phương pháp định tính để tiếp cận nhận thức của các nhân viên y tế đối với việc sàng lọc bằng công cụ này. Tổng cộng 19 cuộc phỏng vấn bán cấu trúc đã được thực hiện tại một Quỹ chăm sóc sức khỏe ban đầu (PCT). Các kết quả đã được phân tích trong bối cảnh các khuyến nghị được đưa ra bởi Unite/CPHVA và Ủy ban sàng lọc quốc gia (NSC). Mặc dù đã được đào tạo tại PCT, một số nhân viên y tế đã sàng lọc trước khi được đào tạo. Tất cả những người tham gia đều cảm thấy rằng EPDS hữu ích, nhưng đối với một số người thì đây chỉ là cách để giới thiệu chủ đề trầm cảm sau sinh. Trong khi một số người cho rằng công cụ này nói chung là tốt, những người khác lại báo cáo có vấn đề với công cụ này, đặc biệt là liên quan đến mục 10 về tự làm hại. Chúng tôi thấy sự khác biệt lớn trong thực hành sàng lọc liên quan đến thời gian, môi trường và sàng lọc lại. Chúng tôi đã đưa ra các đề xuất để thay đổi thực hành để đáp ứng các khuyến nghị của Unite/CPHVA và NSC, đồng thời đảm bảo rằng phụ nữ nhận được cùng một tiêu chuẩn chăm sóc trên toàn PCT."} +{"idx": 1970065, "en": "In December 2007 the European Medicines Agency (EMEA) approved panitumumab (Vectibix) for the treatment of metastatic colorectal cancer. Panitumumab has a conditional approval as monotherapy for the treatment of patients with EGFR-expressing tumours with non-mutated (wild-type) K-ras genes after failure of standard chemotherapy regimens. This specific subgroup of patients showed a more favourable outcome in a controlled, open-label, randomized phase III study. Patients treated with panitumumab plus best supportive care had a significantly prolonged progression-free survival compared to patients receiving best supportive care alone.", "gemini": "Vào tháng 12 năm 2007, Cơ quan Dược phẩm Châu Âu (EMEA) đã chấp thuận panitumumab (Vectibix) để điều trị ung thư đại trực tràng di căn. Panitumumab được chấp thuận có điều kiện như một liệu pháp đơn trị để điều trị bệnh nhân ung thư biểu hiện EGFR với gen K-ras không đột biến (kiểu hoang dã) sau khi thất bại các phác đồ hóa trị liệu chuẩn. Nhóm bệnh nhân đặc biệt này cho thấy kết quả thuận lợi hơn trong một nghiên cứu giai đoạn III ngẫu nhiên, nhãn mở có đối chứng. Những bệnh nhân được điều trị bằng panitumumab cộng với chăm sóc hỗ trợ tốt nhất có thời gian sống không tiến triển kéo dài đáng kể so với những bệnh nhân chỉ được chăm sóc hỗ trợ tốt nhất."} +{"idx": 1970066, "en": "Hantaviruses are enveloped single-stranded ribonucleic acid (RNA) viruses that belong to the family Bunyaviridae. They are widely distributed and maintained in populations of specific insectivores or rodent host species (mice or rats). In their natural host, hantaviruses develop persistent and asymptomatic infections with lifelong virus shedding in excreta. Transmission to humans occurs via direct contact with faeces, urine and saliva of infected rodents, in particular by inhaling virus contaminated aerosol. There are a variety of virus types that occur in different geographic areas, dependent on the natural distribution of the host. Unlike asymptomatic infection in rodents, several types of hantaviruses cause acute febrile diseases in humans, known as haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). In Germany, human hantavirus infections frequently represent as nephropathia epidemica (NE), a generally mild HFRS form with influenza-like symptoms such as fever, myalgia, headache and gastrointestinal disorders. Kidney failure, a characteristic sign of hantavirus infections, is also common. German NE cases are primarily caused by Puumala viruses (PUUV) which naturally persist in the bank vole, a mouse species that is widely distributed in the whole area of Germany. During 2005 and 2007, in several regions of Southern and Western Germany there was an unexpected increase in PUUV-associated NE cases. Currently, 1687 symptomatic hantavirus infections have been reported until the end of 2007, which is the highest number of hantavirus diseases in Germany since the introduction of the German infection protective law (Deutsches Infektionsschutzgesetz) in 2001. Treatment of hantavirus infections is mainly supportive and involves intensive medical care. A specific antiviral therapy with ribavirin has been shown to be successful for treatment of HFRS patients in the early stage of disease. In Germany, there is no licensed vaccine applicable for the prevention of hantavirus diseases. Anti-infectious measures are, therefore, regarded to be the most effective strategy to prevent illnesses due to these emerging RNA viruses.", "gemini": "Hantavirus là virus ARN sợi đơn có vỏ bọc thuộc họ Bunyaviridae. Chúng phân bố rộng rãi và tồn tại trong quần thể các loài côn trùng ăn thịt hoặc loài gặm nhấm vật chủ đặc hiệu (chuột hoặc chuột cống). Ở vật chủ tự nhiên của chúng, hantavirus phát triển thành các bệnh nhiễm trùng dai dẳng và không triệu chứng với việc virus thải ra suốt đời trong phân. Việc lây truyền sang người xảy ra thông qua tiếp xúc trực tiếp với phân, nước tiểu và nước bọt của các loài gặm nhấm bị nhiễm bệnh, đặc biệt là hít phải khí dung có chứa virus. Có nhiều loại virus khác nhau xuất hiện ở các khu vực địa lý khác nhau, tùy thuộc vào sự phân bố tự nhiên của vật chủ. Không giống như nhiễm trùng không triệu chứng ở loài gặm nhấm, một số loại hantavirus gây ra các bệnh sốt cấp tính ở người, được gọi là sốt xuất huyết có hội chứng thận (HFRS) và hội chứng phổi do hantavirus (HPS). Ở Đức, các bệnh nhiễm hantavirus ở người thường biểu hiện dưới dạng bệnh thận dịch tễ (NE), một dạng HFRS thường nhẹ với các triệu chứng giống như cúm như sốt, đau cơ, nhức đầu và rối loạn tiêu hóa. Suy thận, một dấu hiệu đặc trưng của các bệnh nhiễm hantavirus, cũng phổ biến. Các trường hợp NE của Đức chủ yếu do virus Puumala (PUUV) gây ra, loại virus này tồn tại tự nhiên ở chuột đồng ngân hàng, một loài chuột phân bố rộng rãi trên toàn lãnh thổ Đức. Trong năm 2005 và 2007, ở một số vùng của miền Nam và Tây Đức, có sự gia tăng bất ngờ các trường hợp NE liên quan đến PUUV. Hiện tại, tính đến cuối năm 2007, đã có 1687 trường hợp nhiễm hantavirus có triệu chứng được báo cáo, đây là số ca mắc bệnh hantavirus cao nhất ở Đức kể từ khi ban hành luật bảo vệ chống nhiễm trùng của Đức (Deutsches Infektionsschutzgesetz) vào năm 2001. Điều trị các bệnh nhiễm hantavirus chủ yếu là hỗ trợ và bao gồm chăm sóc y tế tích cực. Một liệu pháp kháng vi-rút đặc hiệu với ribavirin đã được chứng minh là thành công trong điều trị bệnh nhân HFRS ở giai đoạn đầu của bệnh. Ở Đức, không có vắc-xin được cấp phép áp dụng để phòng ngừa các bệnh do hantavirus. Do đó, các biện pháp chống nhiễm trùng được coi là chiến lược hiệu quả nhất để ngăn ngừa bệnh tật do các loại virus RNA mới nổi này gây ra."} +{"idx": 1970067, "en": "This is the first in a two-part unit on caring for patients with burns. It focuses on the two main formulas used to produce calculations for fluid resuscitation.", "gemini": "Đây là phần đầu tiên trong loạt bài gồm hai phần về cách chăm sóc bệnh nhân bị bỏng. Bài viết tập trung vào hai công thức chính được sử dụng để tính toán bù dịch."} +{"idx": 1970068, "en": "Properties of a drug for cutaneous application strongly depend on its vehicle. The appropriate excipient should be chosen according to the skin disease and site of application. There are several products for the treatment of atopic dermatitis, which differ in their content of water, lipids and emulsifiers, thus affecting the penetration into the skin. Therefore, individually produced drugs according to the NRF exhibit the optimal features for an individual drug therapy.", "gemini": "Tính chất của thuốc dùng ngoài da phụ thuộc rất nhiều vào tá dược của thuốc. Tá dược thích hợp nên được lựa chọn theo bệnh ngoài da và vị trí dùng thuốc. Có một số sản phẩm điều trị viêm da dị ứng, khác nhau về hàm lượng nước, lipid và chất nhũ hóa, do đó ảnh hưởng đến khả năng thẩm thấu vào da. Do đó, các loại thuốc được sản xuất riêng theo NRF thể hiện các đặc điểm tối ưu cho từng liệu pháp dùng thuốc."} +{"idx": 1970069, "en": "Lower-limb ulceration occurs frequently in patients with rheumatoid arthritis and poses challenges for nurses in terms of assessment and management. Jill Firth examines the underlying aetiology of ulceration in this client group and the implications for nursing practice.", "gemini": "Loét chi dưới thường xảy ra ở những bệnh nhân bị viêm khớp dạng thấp và gây ra những thách thức cho các y tá về việc đánh giá và quản lý. Jill Firth kiểm tra nguyên nhân cơ bản của loét ở nhóm khách hàng này và những tác động đối với hoạt động điều dưỡng."} +{"idx": 1970070, "en": "In the last issue of Chronic Wound Management, Rose Cooper explained the antimicrobial effects of honey (Cooper, 2008). In this article Julie Betts explains how honey is used in practice.", "gemini": "Trong số báo cuối cùng của Tạp chí quản lý vết thương mạn tính, Rose Cooper đã giải thích về tác dụng kháng khuẩn của mật ong (Cooper, 2008). Trong bài viết này, Julie Betts giải thích cách sử dụng mật ong trong thực tế."} +{"idx": 1970071, "en": "beta-Arrestins have important roles in the regulation of seven-transmembrane receptors (7TMRs). Smoothened (Smo) is a 7TMR that mediates effects of Hedgehog on developmental processes and whose dysregulation may cause tumorigenesis. beta-Arrestins are required for endocytosis of Smo and signaling to Gli transcription factors. In mammalian cells, Smo-dependent signaling requires translocation to primary cilia. We demonstrated that beta-arrestins mediate the activity-dependent interaction of Smo and the kinesin motor protein Kif3A. This multimeric complex localized to primary cilia and was disrupted in cells transfected with beta-arrestin small interfering RNA. beta-Arrestin 1 or beta-arrestin 2 depletion prevented the localization of Smo to primary cilia and the Smo-dependent activation of Gli. These results suggest roles for beta-arrestins in mediating the intracellular transport of a 7TMR to its obligate subcellular location for signaling.", "gemini": "beta-Arrestin có vai trò quan trọng trong việc điều hòa các thụ thể xuyên màng bảy (7TMR). Smoothened (Smo) là một 7TMR trung gian cho các tác động của Hedgehog đối với các quá trình phát triển và sự rối loạn điều hòa của nó có thể gây ra ung thư. beta-Arrestin là cần thiết cho quá trình nội bào của Smo và truyền tín hiệu đến các yếu tố phiên mã Gli. Trong các tế bào động vật có vú, tín hiệu phụ thuộc Smo đòi hỏi phải chuyển vị đến lông mao sơ cấp. Chúng tôi đã chứng minh rằng beta-arrestin trung gian cho sự tương tác phụ thuộc hoạt động của Smo và protein vận động kinesin Kif3A. Phức hợp đa phân này định vị tại lông mao sơ cấp và bị phá vỡ trong các tế bào được chuyển gen với RNA can thiệp nhỏ beta-arrestin. Sự suy giảm beta-Arrestin 1 hoặc beta-arrestin 2 ngăn cản sự định vị của Smo đến lông mao sơ cấp và sự hoạt hóa Gli phụ thuộc Smo. Những kết quả này cho thấy vai trò của beta-arrestin trong việc trung gian vận chuyển nội bào của 7TMR đến vị trí dưới tế bào bắt buộc của nó để truyền tín hiệu."} +{"idx": 1970072, "en": "The absorption of atmospheric carbon dioxide (CO2) into the ocean lowers the pH of the waters. This so-called ocean acidification could have important consequences for marine ecosystems. To better understand the extent of this ocean acidification in coastal waters, we conducted hydrographic surveys along the continental shelf of western North America from central Canada to northern Mexico. We observed seawater that is undersaturated with respect to aragonite upwelling onto large portions of the continental shelf, reaching depths of approximately 40 to 120 meters along most transect lines and all the way to the surface on one transect off northern California. Although seasonal upwelling of the undersaturated waters onto the shelf is a natural phenomenon in this region, the ocean uptake of anthropogenic CO2 has increased the areal extent of the affected area.", "gemini": "Sự hấp thụ carbon dioxide (CO2) trong khí quyển vào đại dương làm giảm độ pH của nước. Sự axit hóa đại dương này có thể gây ra hậu quả quan trọng đối với hệ sinh thái biển. Để hiểu rõ hơn về mức độ axit hóa đại dương này ở vùng nước ven biển, chúng tôi đã tiến hành các cuộc khảo sát thủy văn dọc theo thềm lục địa của Bắc Mỹ từ miền trung Canada đến miền bắc Mexico. Chúng tôi quan sát thấy nước biển không bão hòa đối với aragonit trồi lên các phần lớn của thềm lục địa, đạt độ sâu khoảng 40 đến 120 mét dọc theo hầu hết các đường chuyển và kéo dài đến bề mặt trên một đường chuyển ngoài khơi miền bắc California. Mặc dù sự trồi lên theo mùa của các vùng nước không bão hòa lên thềm là một hiện tượng tự nhiên trong khu vực này, nhưng lượng CO2 do con người hấp thụ đã làm tăng diện tích vùng bị ảnh hưởng."} +{"idx": 1970073, "en": "The multiprotein mTORC1 protein kinase complex is the central component of a pathway that promotes growth in response to insulin, energy levels, and amino acids and is deregulated in common cancers. We find that the Rag proteins--a family of four related small guanosine triphosphatases (GTPases)--interact with mTORC1 in an amino acid-sensitive manner and are necessary for the activation of the mTORC1 pathway by amino acids. A Rag mutant that is constitutively bound to guanosine triphosphate interacted strongly with mTORC1, and its expression within cells made the mTORC1 pathway resistant to amino acid deprivation. Conversely, expression of a guanosine diphosphate-bound Rag mutant prevented stimulation of mTORC1 by amino acids. The Rag proteins do not directly stimulate the kinase activity of mTORC1, but, like amino acids, promote the intracellular localization of mTOR to a compartment that also contains its activator Rheb.", "gemini": "Phức hợp protein kinase mTORC1 đa protein là thành phần trung tâm của một con đường thúc đẩy sự tăng trưởng để đáp ứng với insulin, mức năng lượng và axit amin và bị mất điều hòa trong các bệnh ung thư phổ biến. Chúng tôi thấy rằng các protein Rag - một họ gồm bốn guanosine triphosphatase nhỏ liên quan (GTPase) - tương tác với mTORC1 theo cách nhạy cảm với axit amin và cần thiết để kích hoạt con đường mTORC1 bởi axit amin. Một đột biến Rag liên kết liên tục với guanosine triphosphate tương tác mạnh với mTORC1 và sự biểu hiện của nó trong tế bào làm cho con đường mTORC1 trở nên kháng với sự thiếu hụt axit amin. Ngược lại, sự biểu hiện của một đột biến Rag liên kết với guanosine diphosphate ngăn cản sự kích thích mTORC1 bởi axit amin. Các protein Rag không kích thích trực tiếp hoạt động kinase của mTORC1, nhưng giống như axit amin, thúc đẩy định vị nội bào của mTOR đến một ngăn cũng chứa chất hoạt hóa Rheb của nó."} +{"idx": 1970074, "en": "Mammals are metagenomic in that they are composed of not only their own gene complements but also those of all of their associated microbes. To understand the coevolution of the mammals and their indigenous microbial communities, we conducted a network-based analysis of bacterial 16S ribosomal RNA gene sequences from the fecal microbiota of humans and 59 other mammalian species living in two zoos and in the wild. The results indicate that host diet and phylogeny both influence bacterial diversity, which increases from carnivory to omnivory to herbivory; that bacterial communities codiversified with their hosts; and that the gut microbiota of humans living a modern life-style is typical of omnivorous primates.", "gemini": "Động vật có vú là siêu gen vì chúng được tạo thành không chỉ từ các thành phần gen của chính chúng mà còn từ tất cả các vi khuẩn liên quan của chúng. Để hiểu được sự tiến hóa đồng thời của động vật có vú và các cộng đồng vi khuẩn bản địa của chúng, chúng tôi đã tiến hành phân tích dựa trên mạng lưới các trình tự gen RNA ribosome 16S của vi khuẩn từ hệ vi sinh vật đường ruột của con người và 59 loài động vật có vú khác sống trong hai sở thú và trong tự nhiên. Kết quả chỉ ra rằng chế độ ăn của vật chủ và nguồn gốc loài đều ảnh hưởng đến sự đa dạng của vi khuẩn, tăng từ động vật ăn thịt đến động vật ăn tạp đến động vật ăn cỏ; rằng các cộng đồng vi khuẩn tiến hóa đồng thời với vật chủ của chúng; và hệ vi sinh vật đường ruột của con người sống theo lối sống hiện đại là điển hình của loài linh trưởng ăn tạp."} +{"idx": 1970075, "en": "Taguchi et al. (Reports, 20 July 2007, p. 369) reported that mice heterozygous for a null mutation in insulin receptor substrate-2 (Irs2) display a 17% increase in median life span. However, using the same mouse model, we find no evidence for life-span extension and suggest that the findings of Taguchi et al. were due to atypical life-span profiles in their study animals.", "gemini": "Taguchi và cộng sự (Báo cáo, ngày 20 tháng 7 năm 2007, trang 369) đã báo cáo rằng những con chuột có kiểu gen dị hợp tử đối với đột biến mất chức năng ở chất nền thụ thể insulin-2 (Irs2) biểu hiện tuổi thọ trung bình tăng 17%. Tuy nhiên, khi sử dụng cùng một mô hình chuột, chúng tôi không tìm thấy bằng chứng về việc kéo dài tuổi thọ và cho rằng những phát hiện của Taguchi và cộng sự là do hồ sơ tuổi thọ không điển hình ở các động vật nghiên cứu của họ."} +{"idx": 1970076, "en": "Virtually all nonequilibrium electron transfers on Earth are driven by a set of nanobiological machines composed largely of multimeric protein complexes associated with a small number of prosthetic groups. These machines evolved exclusively in microbes early in our planet's history yet, despite their antiquity, are highly conserved. Hence, although there is enormous genetic diversity in nature, there remains a relatively stable set of core genes coding for the major redox reactions essential for life and biogeochemical cycles. These genes created and coevolved with biogeochemical cycles and were passed from microbe to microbe primarily by horizontal gene transfer. A major challenge in the coming decades is to understand how these machines evolved, how they work, and the processes that control their activity on both molecular and planetary scales.", "gemini": "Hầu như tất cả các quá trình truyền electron không cân bằng trên Trái đất đều được thúc đẩy bởi một tập hợp các máy nano sinh học chủ yếu bao gồm các phức hợp protein đa phân tử liên kết với một số ít nhóm chức năng. Những cỗ máy này tiến hóa độc quyền trong vi khuẩn vào giai đoạn đầu trong lịch sử hành tinh của chúng ta, mặc dù chúng có tính cổ xưa nhưng lại được bảo tồn rất cao. Do đó, mặc dù có sự đa dạng di truyền rất lớn trong tự nhiên, vẫn còn một tập hợp các gen cốt lõi tương đối ổn định mã hóa cho các phản ứng oxy hóa khử chính cần thiết cho sự sống và chu trình địa hóa sinh. Những gen này đã tạo ra và cùng tiến hóa với các chu trình địa hóa sinh và được truyền từ vi khuẩn này sang vi khuẩn khác chủ yếu thông qua quá trình truyền gen theo chiều ngang. Một thách thức lớn trong những thập kỷ tới là hiểu cách những cỗ máy này tiến hóa, cách chúng hoạt động và các quá trình kiểm soát hoạt động của chúng ở cả cấp độ phân tử và hành tinh."} +{"idx": 1970077, "en": "The biogeographic variation of life has predominantly been studied using taxonomy, but this focus is changing. There is a resurging interest in understanding patterns in the distribution not only of taxa but also of the traits those taxa possess. Patterns of trait variation shed light on fundamental questions in biology, including why organisms live where they do and how they will respond to environmental change. Technological advances such as environmental genomics place microbial ecology in a unique position to move trait-based biogeography forward. We anticipate that as trait-based biogeography continues to evolve, micro- and macroorganisms will be studied in concert, establishing a science that is informed by and relevant to all domains of life.", "gemini": "Sự biến đổi về mặt địa sinh học của sự sống chủ yếu được nghiên cứu bằng phân loại học, nhưng trọng tâm này đang thay đổi. Có một sự quan tâm mới trong việc hiểu các mô hình phân bố không chỉ của các đơn vị phân loại mà còn của các đặc điểm mà các đơn vị phân loại đó sở hữu. Các mô hình biến đổi đặc điểm làm sáng tỏ các câu hỏi cơ bản trong sinh học, bao gồm lý do tại sao các sinh vật sống ở nơi chúng sống và chúng sẽ phản ứng như thế nào với những thay đổi của môi trường. Những tiến bộ công nghệ như hệ gen học môi trường đưa sinh thái học vi sinh vật vào một vị trí độc nhất để thúc đẩy địa sinh học dựa trên đặc điểm. Chúng tôi dự đoán rằng khi địa sinh học dựa trên đặc điểm tiếp tục phát triển, các vi sinh vật và vĩ sinh vật sẽ được nghiên cứu cùng nhau, thiết lập một khoa học được thông tin và có liên quan đến tất cả các lĩnh vực của sự sống."} +{"idx": 1970078, "en": "The oceans harbor a tremendous diversity of marine microbes. Different functional groups of bacteria, archaea, and protists arise from this diversity to dominate various habitats and drive globally important biogeochemical cycles. Explanations for the distribution of microbial taxa and their associated activity often focus on resource availability and abiotic conditions. However, the continual reshaping of communities by mortality, allelopathy, symbiosis, and other processes shows that community interactions exert strong selective pressure on marine microbes. Deeper exploration of microbial interactions is now possible via molecular prospecting and taxon-specific experimental approaches. A holistic outlook that encompasses the full array of selective pressures on individuals will help elucidate the maintenance of microbial diversity and the regulation of biogeochemical reactions by planktonic communities.", "gemini": "Các đại dương chứa một sự đa dạng to lớn các vi khuẩn biển. Các nhóm chức năng khác nhau của vi khuẩn, vi khuẩn cổ và sinh vật nguyên sinh phát sinh từ sự đa dạng này để thống trị các môi trường sống khác nhau và thúc đẩy các chu trình địa hóa sinh quan trọng trên toàn cầu. Các giải thích về sự phân bố của các loài vi khuẩn và hoạt động liên quan của chúng thường tập trung vào tính sẵn có của tài nguyên và các điều kiện phi sinh học. Tuy nhiên, việc định hình lại liên tục các cộng đồng thông qua tử vong, dị ứng, cộng sinh và các quá trình khác cho thấy các tương tác cộng đồng tạo ra áp lực chọn lọc mạnh mẽ đối với các vi khuẩn biển. Giờ đây, có thể khám phá sâu hơn các tương tác của vi khuẩn thông qua việc thăm dò phân tử và các phương pháp thực nghiệm cụ thể theo loài. Một quan điểm toàn diện bao gồm toàn bộ các áp lực chọn lọc đối với các cá thể sẽ giúp làm sáng tỏ việc duy trì sự đa dạng của vi khuẩn và sự điều chỉnh các phản ứng địa hóa sinh học của các cộng đồng sinh vật phù du."} +{"idx": 1970079, "en": "Sub-sea-floor sediments may contain two-thirds of Earth's total prokaryotic biomass. However, this has its basis in data extrapolation from ~500-meter to 4-kilometer depths, whereas the deepest documented prokaryotes are from only 842 meters. Here, we provide evidence for low concentrations of living prokaryotic cells in the deepest (1626 meters below the sea floor), oldest (111 million years old), and potentially hottest (~100 degrees C) marine sediments investigated. These Newfoundland margin sediments also have DNA sequences related to thermophilic and/or hyperthermophilic Archaea. These form two unique clusters within Pyrococcus and Thermococcus genera, suggesting unknown, uncultured groups are present in deep, hot, marine sediments (~54 degrees to 100 degrees C). Sequences of anaerobic methane-oxidizing Archaea were also present, suggesting a deep biosphere partly supported by methane. These findings demonstrate that the sub-sea-floor biosphere extends to at least 1600 meters below the sea floor and probably deeper, given an upper temperature limit for prokaryotic life of at least 113 degrees C and increasing thermogenic energy supply with depth.", "gemini": "Các trầm tích dưới đáy biển có thể chứa tới hai phần ba tổng lượng sinh khối prokaryotic của Trái đất. Tuy nhiên, điều này có cơ sở từ dữ liệu ngoại suy từ độ sâu ~500 mét đến 4 km, trong khi các prokaryotic được ghi nhận sâu nhất chỉ từ 842 mét. Ở đây, chúng tôi cung cấp bằng chứng về nồng độ thấp các tế bào prokaryotic sống ở độ sâu nhất (1626 mét dưới đáy biển), lâu đời nhất (111 triệu năm tuổi) và có khả năng nóng nhất (~100 độ C) trong các trầm tích biển được nghiên cứu. Các trầm tích ngoài khơi Newfoundland này cũng có trình tự DNA liên quan đến vi khuẩn cổ ưa nhiệt và/hoặc ưa nhiệt độ cực cao. Chúng tạo thành hai nhóm độc đáo trong các chi Pyrococcus và Thermococcus, cho thấy các nhóm chưa biết, chưa nuôi cấy có mặt trong các trầm tích biển sâu, nóng (~54 độ đến 100 độ C). Trình tự của vi khuẩn cổ oxy hóa metan kỵ khí cũng có mặt, cho thấy một tầng sinh quyển sâu được hỗ trợ một phần bởi metan. Những phát hiện này chứng minh rằng tầng sinh quyển dưới đáy biển mở rộng đến ít nhất 1600 mét dưới đáy biển và có thể sâu hơn, với giới hạn nhiệt độ trên đối với sự sống của prokaryotic là ít nhất 113 độ C và nguồn cung cấp năng lượng sinh nhiệt tăng theo độ sâu."} +{"idx": 1970080, "en": "Viruses shape microbial community structure and function by altering the fitness of their hosts and by promoting genetic exchange. The complexity of most natural ecosystems has precluded detailed studies of virus-host interactions. We reconstructed virus and host bacterial and archaeal genome sequences from community genomic data from two natural acidophilic biofilms. Viruses were matched to their hosts by analyzing spacer sequences that occur among clustered regularly interspaced short palindromic repeats (CRISPRs) that are a hallmark of virus resistance. Virus population genomic analyses provided evidence that extensive recombination shuffles sequence motifs sufficiently to evade CRISPR spacers. Only the most recently acquired spacers match coexisting viruses, which suggests that community stability is achieved by rapid but compensatory shifts in host resistance levels and virus population structure.", "gemini": "Virus định hình cấu trúc và chức năng của cộng đồng vi khuẩn bằng cách thay đổi thể lực của vật chủ và bằng cách thúc đẩy trao đổi gen. Sự phức tạp của hầu hết các hệ sinh thái tự nhiên đã ngăn cản các nghiên cứu chi tiết về tương tác giữa virus và vật chủ. Chúng tôi đã tái tạo trình tự bộ gen của virus và vi khuẩn vật chủ và vi khuẩn cổ từ dữ liệu bộ gen cộng đồng từ hai màng sinh học ưa axit tự nhiên. Virus được ghép nối với vật chủ của chúng bằng cách phân tích các trình tự giãn cách xảy ra giữa các đoạn lặp lại palindromic ngắn được xen kẽ theo cụm (CRISPR) là đặc điểm của khả năng kháng virus. Các phân tích bộ gen quần thể virus cung cấp bằng chứng cho thấy sự tái tổ hợp rộng rãi xáo trộn các mô típ trình tự đủ để trốn tránh các đoạn giãn cách CRISPR. Chỉ những đoạn giãn cách mới nhất được thu được mới khớp với các loại virus cùng tồn tại, điều này cho thấy sự ổn định của cộng đồng đạt được thông qua sự thay đổi nhanh chóng nhưng bù trừ trong mức độ kháng của vật chủ và cấu trúc quần thể virus."} +{"idx": 1970081, "en": "Sodium exhibits a pronounced minimum of the melting temperature at approximately 118 gigapascals and 300 kelvin. Using single-crystal high-pressure diffraction techniques, we found that the minimum of the sodium melting curve is associated with a concentration of seven different crystalline phases. Slight changes in pressure and/or temperature induce transitions between numerous structural modifications, several of which are highly complex. The complexity of the phase behavior above 100 gigapascals suggests extraordinary liquid and solid states of sodium at extreme conditions and has implications for other seemingly simple metals.", "gemini": "Natri thể hiện nhiệt độ nóng chảy tối thiểu rõ rệt ở khoảng 118 gigapascal và 300 kelvin. Sử dụng các kỹ thuật nhiễu xạ áp suất cao đơn tinh thể, chúng tôi thấy rằng nhiệt độ nóng chảy tối thiểu của natri liên quan đến nồng độ của bảy pha tinh thể khác nhau. Những thay đổi nhỏ về áp suất và/hoặc nhiệt độ sẽ gây ra các chuyển đổi giữa nhiều biến đổi cấu trúc, một số trong số đó rất phức tạp. Tính phức tạp của hành vi pha trên 100 gigapascal cho thấy trạng thái rắn và lỏng bất thường của natri ở các điều kiện khắc nghiệt và có ý nghĩa đối với các kim loại khác có vẻ đơn giản."} +{"idx": 1970082, "en": "Nitric oxide acts substantially in cellular signal transduction through stimulus-coupled S-nitrosylation of cysteine residues. The mechanisms that might subserve protein denitrosylation in cellular signaling remain uncharacterized. Our search for denitrosylase activities focused on caspase-3, an exemplar of stimulus-dependent denitrosylation, and identified thioredoxin and thioredoxin reductase in a biochemical screen. In resting human lymphocytes, thioredoxin-1 actively denitrosylated cytosolic caspase-3 and thereby maintained a low steady-state amount of S-nitrosylation. Upon stimulation of Fas, thioredoxin-2 mediated denitrosylation of mitochondria-associated caspase-3, a process required for caspase-3 activation, and promoted apoptosis. Inhibition of thioredoxin-thioredoxin reductases enabled identification of additional substrates subject to endogenous S-nitrosylation. Thus, specific enzymatic mechanisms may regulate basal and stimulus-induced denitrosylation in mammalian cells.", "gemini": "Oxit nitric hoạt động đáng kể trong quá trình chuyển tín hiệu tế bào thông qua quá trình S-nitrosyl hóa các gốc cysteine được kích thích bởi kích thích. Các cơ chế có thể phục vụ cho quá trình khử nitơ protein trong quá trình truyền tín hiệu tế bào vẫn chưa được xác định. Cuộc tìm kiếm các hoạt động khử nitơ của chúng tôi tập trung vào caspase-3, một ví dụ điển hình về quá trình khử nitơ phụ thuộc vào kích thích, và xác định thioredoxin và thioredoxin reductase trong quá trình sàng lọc sinh hóa. Trong tế bào lympho người nghỉ ngơi, thioredoxin-1 chủ động khử nitơ caspase-3 trong tế bào chất và do đó duy trì lượng S-nitrosyl hóa ở trạng thái ổn định thấp. Khi kích thích Fas, thioredoxin-2 trung gian quá trình khử nitơ của caspase-3 liên kết với ty thể, một quá trình cần thiết cho hoạt hóa caspase-3 và thúc đẩy quá trình apoptosis. Việc ức chế thioredoxin-thioredoxin reductase cho phép xác định các chất nền bổ sung phải chịu quá trình S-nitrosyl hóa nội sinh. Do đó, các cơ chế enzym cụ thể có thể điều chỉnh quá trình khử nitơ cơ bản và do kích thích gây ra trong các tế bào động vật có vú."} +{"idx": 1970083, "en": "Materials are typically ductile at higher temperatures and become brittle at lower temperatures. In contrast to the typical ductile-to-brittle transition behavior of body-centered cubic (bcc) steels, we observed an inverse temperature dependence of toughness in an ultrahigh-strength bcc steel with an ultrafine elongated ferrite grain structure that was processed by a thermomechanical treatment without the addition of a large amount of an alloying element. The enhanced toughness is attributed to a delamination that was a result of crack branching on the aligned {100} cleavage planes in the bundles of the ultrafine elongated ferrite grains strengthened by nanometer-sized carbides. In the temperature range from 60 degrees to -60 degrees C, the yield strength was greater, leading to the enhancement of the toughness.", "gemini": "Vật liệu thường dẻo ở nhiệt độ cao hơn và trở nên giòn ở nhiệt độ thấp hơn. Trái ngược với hành vi chuyển đổi từ dẻo sang giòn điển hình của thép lập phương tâm khối (bcc), chúng tôi quan sát thấy sự phụ thuộc nhiệt độ nghịch đảo của độ dai trong thép bcc có độ bền cực cao với cấu trúc hạt ferit kéo dài cực mịn được gia công bằng xử lý nhiệt cơ mà không cần thêm một lượng lớn nguyên tố hợp kim. Độ dai tăng cường được cho là do sự phân tách lớp là kết quả của sự phân nhánh vết nứt trên các mặt phẳng phân cắt {100} thẳng hàng trong các bó hạt ferit kéo dài cực mịn được gia cố bằng các carbide kích thước nanomet. Trong phạm vi nhiệt độ từ 60 độ đến -60 độ C, độ bền chảy lớn hơn, dẫn đến tăng độ dai."} +{"idx": 1970084, "en": "Mineral deposits on the martian surface can elucidate ancient environmental conditions on the planet. Opaline silica deposits (as much as 91 weight percent SiO2) have been found in association with volcanic materials by the Mars rover Spirit. The deposits are present both as light-toned soils and as bedrock. We interpret these materials to have formed under hydrothermal conditions and therefore to be strong indicators of a former aqueous environment. This discovery is important for understanding the past habitability of Mars because hydrothermal environments on Earth support thriving microbial ecosystems.", "gemini": "Các khoáng chất lắng đọng trên bề mặt sao Hỏa có thể làm sáng tỏ các điều kiện môi trường cổ đại trên hành tinh này. Các mỏ silica opal (nhiều đến 91 phần trăm khối lượng SiO2) đã được phát hiện có liên quan đến các vật liệu núi lửa bởi tàu thám hiểm Spirit của sao Hỏa. Các mỏ này có mặt ở dạng đất có tông màu nhạt và dạng nền đá. Chúng tôi diễn giải các vật liệu này được hình thành trong điều kiện thủy nhiệt và do đó là những chỉ báo mạnh mẽ về một môi trường nước trước đây. Khám phá này rất quan trọng để hiểu được khả năng sinh sống trong quá khứ của sao Hỏa vì các môi trường thủy nhiệt trên Trái đất hỗ trợ các hệ sinh thái vi sinh vật phát triển mạnh."} +{"idx": 1970085, "en": "Amphitheater-headed canyons have been used as diagnostic indicators of erosion by groundwater seepage, which has important implications for landscape evolution on Earth and astrobiology on Mars. Of perhaps any canyon studied, Box Canyon, Idaho, most strongly meets the proposed morphologic criteria for groundwater sapping because it is incised into a basaltic plain with no drainage network upstream, and approximately 10 cubic meters per second of seepage emanates from its vertical headwall. However, sediment transport constraints, 4He and 14C dates, plunge pools, and scoured rock indicate that a megaflood (greater than 220 cubic meters per second) carved the canyon about 45,000 years ago. These results add to a growing recognition of Quaternary catastrophic flooding in the American northwest, and may imply that similar features on Mars also formed by floods rather than seepage erosion.", "gemini": "Các hẻm núi có đầu hình đấu trường đã được sử dụng như những chỉ báo chẩn đoán về sự xói mòn do nước ngầm rỉ ra, điều này có ý nghĩa quan trọng đối với quá trình tiến hóa cảnh quan trên Trái đất và sinh học vũ trụ trên sao Hỏa. Trong số tất cả các hẻm núi đã nghiên cứu, có lẽ Hẻm núi Box, Idaho, đáp ứng mạnh mẽ nhất các tiêu chí về hình thái được đề xuất cho sự rỉ nước ngầm vì nó được khắc vào một đồng bằng bazan không có mạng lưới thoát nước ở thượng nguồn và khoảng 10 mét khối nước ngầm mỗi giây tỏa ra từ vách đầu thẳng đứng của nó. Tuy nhiên, các ràng buộc về vận chuyển trầm tích, ngày tháng 4He và 14C, các hố xoáy và đá bị bào mòn cho thấy một trận đại hồng thủy (lớn hơn 220 mét khối mỗi giây) đã tạo ra hẻm núi cách đây khoảng 45.000 năm. Những kết quả này bổ sung vào sự công nhận ngày càng tăng về trận đại hồng thủy thảm khốc ở phía tây bắc nước Mỹ trong kỷ Đệ tứ, và có thể ngụ ý rằng các đặc điểm tương tự trên sao Hỏa cũng được hình thành do lũ lụt chứ không phải do sự xói mòn do rỉ nước."} +{"idx": 1970086, "en": "Earth's lowermost mantle has thermal, chemical, and mineralogical complexities that require precise seismological characterization. Stacking, migration, and modeling of over 10,000 P and S waves that traverse the deep mantle under the Cocos plate resolve structures above the core-mantle boundary. A small -0.07 +/- 0.15% decrease of P wave velocity (Vp) is accompanied by a 1.5 +/- 0.5% increase in S wave velocity (V(s)) near a depth of 2570 km. Bulk-sound velocity [Vb = (Vp2 - 4/3Vs2)1/2] decreases by -1.0 +/- 0.5% at this depth. Transition of the primary lower-mantle mineral, (Mg(1-x-y) Fe(x)Al(y))(Si,Al)O3 perovskite, to denser post-perovskite is expected to have a negligible effect on the bulk modulus while increasing the shear modulus by approximately 6%, resulting in local anticorrelation of Vb and Vs anomalies; this behavior explains the data well.", "gemini": "Lớp phủ dưới cùng của Trái đất có độ phức tạp về nhiệt, hóa học và khoáng vật đòi hỏi phải có đặc điểm địa chấn chính xác. Việc xếp chồng, di chuyển và mô hình hóa hơn 10.000 sóng P và S đi qua lớp phủ sâu bên dưới mảng Cocos giải quyết các cấu trúc phía trên ranh giới lõi-lớp phủ. Một sự giảm nhỏ -0,07 +/- 0,15% vận tốc sóng P (Vp) đi kèm với sự gia tăng 1,5 +/- 0,5% vận tốc sóng S (V(s)) gần độ sâu 2570 km. Vận tốc âm thanh khối lượng [Vb = (Vp2 - 4/3Vs2)1/2] giảm -1,0 +/- 0,5% ở độ sâu này. Sự chuyển đổi của khoáng vật chính của lớp phủ dưới, perovskite (Mg(1-x-y) Fe(x)Al(y))(Si,Al)O3, thành hậu perovskite đặc hơn dự kiến sẽ có tác động không đáng kể đến mô đun khối trong khi tăng mô đun cắt khoảng 6%, dẫn đến sự tương quan cục bộ nghịch đảo của các bất thường Vb và Vs; hành vi này giải thích tốt dữ liệu."} +{"idx": 1970087, "en": "When food is plentiful, circadian rhythms of animals are powerfully entrained by the light-dark cycle. However, if animals have access to food only during their normal sleep cycle, they will shift most of their circadian rhythms to match the food availability. We studied the basis for entrainment of circadian rhythms by food and light in mice with targeted disruption of the clock gene Bmal1, which lack circadian rhythmicity. Injection of a viral vector containing the Bmal1 gene into the suprachiasmatic nuclei of the hypothalamus restored light-entrainable, but not food-entrainable, circadian rhythms. In contrast, restoration of the Bmal1 gene only in the dorsomedial hypothalamic nucleus restored the ability of animals to entrain to food but not to light. These results demonstrate that the dorsomedial hypothalamus contains a Bmal1-based oscillator that can drive food entrainment of circadian rhythms.", "gemini": "Khi thức ăn dồi dào, nhịp sinh học của động vật bị cuốn theo chu kỳ sáng tối. Tuy nhiên, nếu động vật chỉ tiếp cận được thức ăn trong chu kỳ ngủ bình thường của chúng, chúng sẽ chuyển phần lớn nhịp sinh học của mình để phù hợp với khả năng tiếp cận thức ăn. Chúng tôi đã nghiên cứu cơ sở để cuốn theo nhịp sinh học bằng thức ăn và ánh sáng ở những con chuột bị phá vỡ gen đồng hồ Bmal1, không có nhịp sinh học. Tiêm một vectơ vi-rút chứa gen Bmal1 vào nhân trên thị giác dưới của vùng dưới đồi đã phục hồi nhịp sinh học có thể cuốn theo ánh sáng, nhưng không phải nhịp sinh học có thể cuốn theo thức ăn. Ngược lại, việc phục hồi gen Bmal1 chỉ trong nhân dưới đồi lưng giữa đã phục hồi khả năng cuốn theo thức ăn của động vật nhưng không phải theo ánh sáng. Những kết quả này chứng minh rằng vùng dưới đồi lưng giữa chứa một dao động dựa trên Bmal1 có thể thúc đẩy nhịp sinh học cuốn theo thức ăn."} +{"idx": 1970088, "en": "BACKGROUND AND PURPOSE The objective of this study was to create guidelines for the use of strengthening exercises in the management of adult patients (>18 years of age) with fibromyalgia (FM), as defined by the 1990 American College of Rheumatology criteria. METHODS Following Cochrane Collaboration methods, the Ottawa Methods Group found and synthesized evidence from comparative controlled trials and formed the Ottawa Panel, with nominated experts from key stakeholder organizations. The Ottawa Panel then developed criteria for grading the recommendations based on experimental design (I for randomized controlled trials, II for nonrandomized studies) and strength of evidence (A, B, C+, C, D+, D, or D-). From the rigorous literature search, 5 randomized controlled trials were selected. Statistical analysis was based on Cochrane Collaboration methods. Continuous data were calculated with weighted mean differences between the intervention and control groups, and dichotomous data were analyzed with relative risks. Clinical improvement was calculated using absolute benefit and relative difference in change from baseline. Clinical significance was attained when an improvement of 15% relative to a control was found. RESULTS There were 5 positive recommendations: 2 grade A and 3 grade C+. All 5 were of clinical benefit. DISCUSSION AND CONCLUSION The Ottawa Panel recommends strengthening exercises for the management of fibromyalgia as a result of the emerging evidence (grades A, B, and C+, although most trials were rated low quality) shown in the literature.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Mục tiêu của nghiên cứu này là tạo ra các hướng dẫn sử dụng các bài tập tăng cường sức mạnh trong việc quản lý bệnh nhân trưởng thành (>18 tuổi) bị đau xơ cơ (FM), theo định nghĩa của tiêu chuẩn của Cao đẳng thấp khớp Hoa Kỳ năm 1990. PHƯƠNG PHÁP Theo các phương pháp của Cochrane Collaboration, Nhóm phương pháp Ottawa đã tìm thấy và tổng hợp bằng chứng từ các thử nghiệm có đối chứng so sánh và thành lập Ban Ottawa, với các chuyên gia được đề cử từ các tổ chức có liên quan chính. Sau đó, Ban Ottawa đã phát triển các tiêu chí để phân loại các khuyến nghị dựa trên thiết kế thử nghiệm (I đối với các thử nghiệm có đối chứng ngẫu nhiên, II đối với các nghiên cứu không ngẫu nhiên) và sức mạnh của bằng chứng (A, B, C+, C, D+, D hoặc D-). Từ quá trình tìm kiếm tài liệu nghiêm ngặt, 5 thử nghiệm có đối chứng ngẫu nhiên đã được chọn. Phân tích thống kê dựa trên các phương pháp của Cochrane Collaboration. Dữ liệu liên tục được tính bằng chênh lệch trung bình có trọng số giữa nhóm can thiệp và nhóm đối chứng và dữ liệu phân đôi được phân tích bằng rủi ro tương đối. Cải thiện lâm sàng được tính bằng lợi ích tuyệt đối và chênh lệch tương đối khi thay đổi so với ban đầu. Ý nghĩa lâm sàng đạt được khi tìm thấy sự cải thiện 15% so với đối chứng. KẾT QUẢ Có 5 khuyến nghị tích cực: 2 loại A và 3 loại C+. Cả 5 đều có lợi ích lâm sàng. THẢO LUẬN VÀ KẾT LUẬN Ban Ottawa khuyến nghị các bài tập tăng cường sức mạnh để quản lý đau xơ cơ do bằng chứng mới nổi (hạng A, B và C+, mặc dù hầu hết các thử nghiệm được đánh giá là chất lượng thấp) được thể hiện trong tài liệu."} +{"idx": 1970089, "en": "Angiotensin II activates two distinct receptors, the angiotensin II receptors type 1 (AT(1)) and type 2 (AT(2)). In rodents, two AT(1) subtypes were identified (AT(1a) and AT(1b)). To determine receptor-specific functions and possible angiotensin II effects independent of its three known receptors we generated mice deficient in either one of the angiotensin II receptors, in two, or in all three (triple knockouts). Triple knockouts were vital and fertile, but survival was impaired. Hypotension and renal histological abnormalities in triple knockouts were comparable to those in mice lacking both AT(1) subtypes. All combinations lacking AT(1a) were distinguished by reduced heart rate. AT(1a) deletion impaired the in vivo pressor response to angiotensin II bolus injection, whereas deficiency for AT(1b) and/or AT(2) had no effect. However, the additional lack of AT(1b) in AT(1a)-deficient mice further impaired the vasoconstrictive capacity of angiotensin II. Although general vasoconstrictor properties were not changed, angiotensin II failed to alter blood pressure in triple knockouts, indicating that there are no other receptors involved in direct angiotensin II pressor effects. Our data identify mice deficient in all three angiotensin II receptors as an ideal tool to better understand the structure and function of the renin-angiotensin system and to search for angiotensin II effects independent of AT(1) and AT(2).", "gemini": "Angiotensin II kích hoạt hai thụ thể riêng biệt, thụ thể angiotensin II loại 1 (AT (1)) và loại 2 (AT (2)). Ở loài gặm nhấm, hai phân nhóm AT (1) đã được xác định (AT (1a) và AT (1b)). Để xác định các chức năng riêng của thụ thể và các tác dụng có thể có của angiotensin II độc lập với ba thụ thể đã biết của nó, chúng tôi đã tạo ra những con chuột bị thiếu một trong hai thụ thể angiotensin II, ở hai hoặc ở cả ba (loại bỏ ba lần). Những con chuột bị loại bỏ ba lần vẫn sống và sinh sản, nhưng khả năng sống sót bị suy yếu. Tụt huyết áp và bất thường về mô học thận ở những con chuột bị loại bỏ ba lần tương đương với những con chuột thiếu cả hai phân nhóm AT (1). Tất cả các tổ hợp không có AT (1a) đều được phân biệt bằng nhịp tim giảm. Xóa AT (1a) làm suy yếu phản ứng tăng huyết áp in vivo đối với tiêm bolus angiotensin II, trong khi thiếu AT (1b) và/hoặc AT (2) không có tác dụng. Tuy nhiên, việc thiếu thêm AT (1b) ở những con chuột thiếu AT (1a) làm suy yếu thêm khả năng co mạch của angiotensin II. Mặc dù các đặc tính co mạch nói chung không thay đổi, angiotensin II không làm thay đổi huyết áp ở những con chuột bị loại bỏ ba lần, cho thấy không có thụ thể nào khác tham gia vào các tác dụng tăng huyết áp trực tiếp của angiotensin II. Dữ liệu của chúng tôi xác định những con chuột bị thiếu cả ba thụ thể angiotensin II là một công cụ lý tưởng để hiểu rõ hơn về cấu trúc và chức năng của hệ renin-angiotensin và để tìm kiếm các tác dụng của angiotensin II độc lập với AT (1) và AT (2)."} +{"idx": 1970090, "en": "Identifying ecologically differentiated populations within complex microbial communities remains challenging, yet is critical for interpreting the evolution and ecology of microbes in the wild. Here we describe spatial and temporal resource partitioning among Vibrionaceae strains coexisting in coastal bacterioplankton. A quantitative model (AdaptML) establishes the evolutionary history of ecological differentiation, thus revealing populations specific for seasons and life-styles (combinations of free-living, particle, or zooplankton associations). These ecological population boundaries frequently occur at deep phylogenetic levels (consistent with named species); however, recent and perhaps ongoing adaptive radiation is evident in Vibrio splendidus, which comprises numerous ecologically distinct populations at different levels of phylogenetic differentiation. Thus, environmental specialization may be an important correlate or even trigger of speciation among sympatric microbes.", "gemini": "Việc xác định các quần thể được phân biệt về mặt sinh thái trong các cộng đồng vi khuẩn phức tạp vẫn còn là một thách thức, nhưng lại rất quan trọng để diễn giải quá trình tiến hóa và sinh thái của vi khuẩn trong tự nhiên. Ở đây, chúng tôi mô tả sự phân chia tài nguyên theo không gian và thời gian giữa các chủng Vibrionaceae cùng tồn tại trong vi khuẩn phù du ven biển. Một mô hình định lượng (AdaptML) thiết lập lịch sử tiến hóa của sự phân biệt về mặt sinh thái, do đó tiết lộ các quần thể cụ thể cho các mùa và lối sống (kết hợp các liên kết sống tự do, hạt hoặc động vật phù du). Các ranh giới quần thể sinh thái này thường xảy ra ở các mức độ phát sinh loài sâu (tương thích với các loài được đặt tên); tuy nhiên, sự bức xạ thích nghi gần đây và có lẽ vẫn đang diễn ra là bằng chứng trong Vibrio splendidus, bao gồm nhiều quần thể sinh thái riêng biệt ở các mức độ phân biệt về mặt phát sinh loài khác nhau. Do đó, sự chuyên biệt hóa về mặt môi trường có thể là một tương quan quan trọng hoặc thậm chí là yếu tố kích hoạt quá trình hình thành loài giữa các vi khuẩn cùng loài."} +{"idx": 1970091, "en": "BACKGROUND Generation of physiologically active vascular beds by delivery of combinations of growth factors offers promise for vascular gene therapy. METHODS AND RESULTS In a mesenteric model of physiological angiogenesis, combining endothelial nitric oxide synthase (eNOS) (and hence NO production) with VEGF and angiopoietin-1 overexpression resulted in a more functional vascular phenotype than growth factor administration alone. eNOS gene delivery upregulated eNOS, VEGF, and Ang-1 to similar levels as gene transfer with VEGF or Ang-1. eNOS overexpression resulted in neovascularization to a similar extent as VEGF and Ang-1 combined, but not by sprouting angiogenesis. Whereas combining Ang-1 and VEGF increased both exchange vessels and conduit vessels, neither growth factor nor eNOS alone resulted in vessels with smooth muscle cell (SMC) coverage. In contrast, combining all three generated microvessels with SMCs (arteriolar genesis) and further increased functional vessels. Use of a vasodilator, prazosin, in combination with Ang1 and VEGF, but not alone, also generated SMC-positive vessels. CONCLUSIONS Coexpression of eNOS, VEGF, and Ang-1 results in a more mature vascularization of connective tissue, and generates new arterioles as well as new capillaries, and provides a more physiological therapeutic approach than single growth factor administration, by combining hemodynamic forces with growth factors.", "gemini": "BỐI CẢNH Việc tạo ra các mạch máu hoạt động về mặt sinh lý bằng cách cung cấp kết hợp các yếu tố tăng trưởng mang lại triển vọng cho liệu pháp gen mạch máu. PHƯƠNG PHÁP VÀ KẾT QUẢ Trong mô hình sinh lý học của quá trình sinh mạch, việc kết hợp synthase oxit nitric nội mô (eNOS) (và do đó là sản xuất NO) với VEGF và biểu hiện quá mức angiopoietin-1 dẫn đến kiểu hình mạch máu chức năng hơn so với chỉ dùng yếu tố tăng trưởng. Cung cấp gen eNOS đã điều chỉnh tăng eNOS, VEGF và Ang-1 ở mức độ tương tự như chuyển gen với VEGF hoặc Ang-1. Biểu hiện quá mức eNOS dẫn đến sự tân mạch hóa ở mức độ tương tự như kết hợp VEGF và Ang-1, nhưng không phải bằng cách sinh mạch chồi. Trong khi kết hợp Ang-1 và VEGF làm tăng cả mạch trao đổi và mạch dẫn, thì không có yếu tố tăng trưởng hoặc eNOS nào chỉ tạo ra các mạch có phủ tế bào cơ trơn (SMC). Ngược lại, kết hợp cả ba yếu tố này tạo ra các mạch máu nhỏ có SMC (sinh động mạch) và làm tăng thêm các mạch máu chức năng. Sử dụng thuốc giãn mạch, prazosin, kết hợp với Ang1 và VEGF, nhưng không phải đơn lẻ, cũng tạo ra các mạch máu có SMC dương tính. KẾT LUẬN Việc biểu hiện đồng thời eNOS, VEGF và Ang-1 dẫn đến quá trình mạch máu hóa mô liên kết trưởng thành hơn và tạo ra các tiểu động mạch cũng như mao mạch mới, đồng thời cung cấp phương pháp điều trị sinh lý hơn so với việc chỉ sử dụng yếu tố tăng trưởng, bằng cách kết hợp các lực huyết động với các yếu tố tăng trưởng."} +{"idx": 1970092, "en": "OBJECTIVE Acute coronary diseases are characterized by elevated levels of circulating platelet-leukocyte complexes, raising the possibility that proinflammatory processes might be initiated in leukocytes after platelet adhesion. Here we examined the mechanism of platelet binding to polymorphonuclear leukocytes, monocytes, and monocyte subsets and investigated the potential functional consequences of monocyte binding to minimally activated or thrombin-activated platelets. METHODS AND RESULTS In this article, we describe key differences in terms of stability of PSGL-1-mediated interaction of platelets with monocytes and polymorphonuclear leukocytes and a small but significant difference in platelet binding to monocyte subsets (CD14(high) and CD14(low)/HLA-DR(high)). We also report differential effects of platelet binding on monocyte functional responses between minimally and thrombin-activated platelets. In particular, monocyte CD11b expression and release of proinflammatory cytokines, like interleukin 1beta and tumor necrosis factor alpha, were significantly upregulated on adhesion of stimulated platelets, whereas unstimulated platelets had no effect. Moreover, binding of unstimulated, but not of thrombin-activated, platelets to monocytes had no impact on NF-kappaB activity, monocyte migration, and induction of apoptosis in the absence of survival factors. CONCLUSIONS Our data suggest that in the absence of overt activation, PSGL-1-P-selectin-dependent platelet binding to monocytes represents a normal physiological process with little impact on the potential of monocytes to cause vascular injury.", "gemini": "MỤC TIÊU Các bệnh động mạch vành cấp tính được đặc trưng bởi nồng độ phức hợp tiểu cầu-bạch cầu lưu hành tăng cao, làm tăng khả năng các quá trình tiền viêm có thể bắt đầu ở bạch cầu sau khi tiểu cầu kết dính. Ở đây, chúng tôi đã kiểm tra cơ chế liên kết tiểu cầu với bạch cầu đa nhân trung tính, đơn bào và các phân nhóm đơn bào và nghiên cứu các hậu quả chức năng tiềm tàng của việc liên kết đơn bào với tiểu cầu được hoạt hóa tối thiểu hoặc hoạt hóa bằng thrombin. PHƯƠNG PHÁP VÀ KẾT QUẢ Trong bài báo này, chúng tôi mô tả những điểm khác biệt chính về sự ổn định của tương tác do PSGL-1 trung gian của tiểu cầu với các đơn bào và bạch cầu đa nhân trung tính và một sự khác biệt nhỏ nhưng có ý nghĩa về sự liên kết tiểu cầu với các phân nhóm đơn bào (CD14 (cao) và CD14 (thấp) / HLA-DR (cao)). Chúng tôi cũng báo cáo các hiệu ứng khác nhau của liên kết tiểu cầu đối với các phản ứng chức năng của đơn bào giữa tiểu cầu được hoạt hóa tối thiểu và tiểu cầu được hoạt hóa bằng thrombin. Đặc biệt, biểu hiện CD11b của đơn bào và giải phóng các cytokine tiền viêm, như interleukin 1beta và yếu tố hoại tử khối u alpha, đã được điều chỉnh tăng đáng kể khi kết dính tiểu cầu được kích thích, trong khi tiểu cầu không được kích thích không có tác dụng. Hơn nữa, sự liên kết của tiểu cầu không được kích thích, nhưng không phải tiểu cầu được hoạt hóa bằng thrombin, với các đơn bào không ảnh hưởng đến hoạt động của NF-kappaB, sự di chuyển của đơn bào và quá trình gây apoptosis khi không có các yếu tố sống còn. KẾT LUẬN Dữ liệu của chúng tôi cho thấy rằng khi không có sự hoạt hóa rõ ràng, sự liên kết tiểu cầu phụ thuộc PSGL-1-P-selectin với các đơn bào đại diện cho một quá trình sinh lý bình thường có ít tác động đến khả năng gây tổn thương mạch máu của các đơn bào."} +{"idx": 1970093, "en": "OBJECTIVE The aim of this study was to examine the endothelial distribution and activity of selected PKC isoforms in coronary vessels with respect to their functional impact on endothelial permeability under the experimental conditions relevant to diabetes. METHODS AND RESULTS En face immunohistochemistry demonstrated a significant increase of PKC(betaII) and decrease of PKCdelta expression in coronary arterial endothelium of Zucker diabetic rats. To test whether changes in PKC expression alter endothelial barrier properties, we measured the transcellular electric resistance in human coronary microvascular endothelial monolayers and found that either PKC(betaII) overexpression or PKCdelta inhibition disrupted the cell-cell adhesive barrier. Three-dimensional fluorescence microscopy revealed that hyperpermeability was caused by altered PKC activity in association with distinct translocation of PKC(betaII) to the cell-cell junction and PKCdelta localization to the cytosol. Further analyses in fractionated endothelial lysates confirmed the differential redistribution of these isozymes. Additionally, FRET analysis of PKC subcellular dynamics demonstrated a high PKC(betaII) activity at the cell surface and junction, whereas PKCdelta activity is concentrated in intracellular membrane organelles. CONCLUSIONS Taken together, these data suggest that PKC(betaII) and PKCdelta counter-regulate coronary endothelial barrier properties by targeting distinctive subcellular sites. Imbalanced PKC(betaII)/PKCdelta expression and activity may contribute to endothelial hyperpermeability and coronary dysfunction in diabetes.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu này là kiểm tra sự phân bố nội mô và hoạt động của các isoform PKC được chọn trong các mạch vành liên quan đến tác động chức năng của chúng lên tính thấm nội mô trong các điều kiện thử nghiệm có liên quan đến bệnh tiểu đường. PHƯƠNG PHÁP VÀ KẾT QUẢ Miễn dịch mô hóa học en face chứng minh sự gia tăng đáng kể của PKC(betaII) và giảm biểu hiện PKCdelta trong nội mô động mạch vành của chuột mắc bệnh tiểu đường Zucker. Để kiểm tra xem những thay đổi về biểu hiện PKC có làm thay đổi các đặc tính hàng rào nội mô hay không, chúng tôi đã đo điện trở điện xuyên tế bào trong các lớp nội mô vi mạch vành của người và thấy rằng sự biểu hiện quá mức của PKC(betaII) hoặc ức chế PKCdelta đều phá vỡ hàng rào kết dính tế bào-tế bào. Kính hiển vi huỳnh quang ba chiều cho thấy tính thấm quá mức là do hoạt động PKC thay đổi liên quan đến sự chuyển vị riêng biệt của PKC(betaII) đến mối nối tế bào-tế bào và vị trí PKCdelta đến tế bào chất. Các phân tích thêm trong các chất phân giải nội mô đã xác nhận sự phân phối lại khác biệt của các isozyme này. Ngoài ra, phân tích FRET về động lực dưới tế bào của PKC chứng minh hoạt động PKC(betaII) cao ở bề mặt tế bào và mối nối, trong khi hoạt động PKCdelta tập trung ở các bào quan màng trong tế bào. KẾT LUẬN Kết hợp lại, những dữ liệu này cho thấy PKC(betaII) và PKCdelta điều chỉnh ngược lại các đặc tính hàng rào nội mô vành bằng cách nhắm vào các vị trí dưới tế bào riêng biệt. Biểu hiện và hoạt động mất cân bằng của PKC(betaII)/PKCdelta có thể góp phần vào tính thấm quá mức của nội mô và rối loạn chức năng vành trong bệnh tiểu đường."} +{"idx": 1970094, "en": "OBJECTIVE Discoidin domain receptor 2 (DDR2) plays potential roles in the regulation of collagen turnover mediated by smooth muscle cells (SMCs) in atherosclerosis. Little is known about the function of DDR2 in vascular system. We investigated whether inhibition of DDR2 by small interfering RNA (siRNA) can reduce neointimal formation after arterial injury. METHODS AND RESULTS SMCs from thoracic aorta of adult Wistar rats were cultured. The carotid artery from adult Wistar rats was injured by balloon catheter. DDR2 significantly increased migration and proliferation of SMCs. DDR2 siRNA inhibited 86% of DDR2 protein expression in cultured SMCs. DDR2 protein and mRNA expression significantly increased at 14 days after carotid injury. DDR2 siRNA significantly reduced DDR2 protein and mRNA expression induced by balloon injury. The immunohistochemical stain demonstrated that DDR2 siRNA decreased MMP2 protein labeling induced by balloon injury, a pattern similar to that of DDR2 protein labeling. Neointimal area was significantly increased after carotid injury and was significantly reduced by DDR2 siRNA. CONCLUSIONS DDR2 increases migration and proliferation of SMCs, and expression of DDR2 in carotid artery significantly increases after injury. DDR2 siRNA attenuates neointimal formation after carotid injury. DDR2 may play a pivotal role in the pathogenesis of neointimal thickening after mechanical injury.", "gemini": "MỤC TIÊU thụ thể miền Discoidin 2 (DDR2) đóng vai trò tiềm năng trong việc điều chỉnh sự chuyển hóa collagen do các tế bào cơ trơn (SMC) trong xơ vữa động mạch. Người ta biết rất ít về chức năng của DDR2 trong hệ thống mạch máu. Chúng tôi đã nghiên cứu xem liệu việc ức chế DDR2 bằng RNA can thiệp nhỏ (siRNA) có thể làm giảm sự hình thành tân nội mạc sau chấn thương động mạch hay không. PHƯƠNG PHÁP VÀ KẾT QUẢ Các SMC từ động mạch chủ ngực của chuột Wistar trưởng thành đã được nuôi cấy. Động mạch cảnh từ chuột Wistar trưởng thành bị thương do ống thông bóng. DDR2 làm tăng đáng kể sự di chuyển và tăng sinh của SMC. DDR2 siRNA ức chế 86% biểu hiện protein DDR2 trong các SMC được nuôi cấy. Biểu hiện protein DDR2 và mRNA tăng đáng kể sau 14 ngày bị thương động mạch cảnh. DDR2 siRNA làm giảm đáng kể biểu hiện protein DDR2 và mRNA do chấn thương bóng gây ra. Nhuộm miễn dịch mô đã chứng minh rằng DDR2 siRNA làm giảm nhãn protein MMP2 do chấn thương bóng gây ra, một kiểu tương tự như nhãn protein DDR2. Diện tích tân nội mạc tăng đáng kể sau chấn thương động mạch cảnh và giảm đáng kể bởi DDR2 siRNA. KẾT LUẬN DDR2 làm tăng sự di chuyển và tăng sinh của SMC và biểu hiện của DDR2 trong động mạch cảnh tăng đáng kể sau chấn thương. DDR2 siRNA làm giảm sự hình thành tân nội mạc sau chấn thương động mạch cảnh. DDR2 có thể đóng một vai trò quan trọng trong quá trình sinh bệnh của sự dày lên tân nội mạc sau chấn thương cơ học."} +{"idx": 1970095, "en": "OBJECTIVE We used genetically engineered mouse hematopoietic progenitor cells (HPCs) to investigate the therapeutic effects of human apoAI on atherosclerosis in apoE(-/-) mice. METHODS AND RESULTS Lentiviral constructs expressing either human apoAI (LV-apoAI) or green fluorescent protein (LV-GFP) cDNA under a macrophage specific promoter (CD68) were generated and used for ex vivo transduction of mouse HPCs and macrophages. The transduction efficiency was >25% for HPCs and >70% for macrophages. ApoAI was found in the macrophage culture media, mostly associated with the HDL fraction. Interestingly, a significant increase in mRNA and protein levels for ATP binding cassette A1 (ABCA1) and ABCG1 were found in apoAI-expressing macrophages after acLDL loading. Expression of apoAI significantly increased cholesterol efflux in wild-type and apoE(-/-) macrophages. HPCs transduced with LV-apoAI ex vivo and then transplanted into apoE(-/-) mice caused a 50% reduction in atherosclerotic lesion area compared to GFP controls, without influencing plasma HDL-C levels. CONCLUSIONS Lentiviral transduction of apoAI into HPCs reduces atherosclerosis in apoE(-/-) mice. Expression of apoAI in macrophages improves cholesterol trafficking in wild-type apoE-producing macrophages and causes upregulation of ABCA1 and ABCG1. These novel observations set the stage for a cell therapy approach to atherosclerosis regression, exploiting the cooperation between apoE and apoAI to maximize cholesterol exit from the plaque.", "gemini": "MỤC TIÊU Chúng tôi đã sử dụng các tế bào tiền thân tạo máu (HPC) của chuột được biến đổi gen để nghiên cứu các tác dụng điều trị của apoAI của người đối với xơ vữa động mạch ở chuột apoE(-/-). PHƯƠNG PHÁP VÀ KẾT QUẢ Các cấu trúc lentivirus biểu hiện cDNA apoAI của người (LV-apoAI) hoặc protein huỳnh quang xanh lục (LV-GFP) theo một chất thúc đẩy đặc hiệu của đại thực bào (CD68) đã được tạo ra và được sử dụng để chuyển đổi ngoài cơ thể các HPC và đại thực bào của chuột. Hiệu quả chuyển đổi là >25% đối với HPC và >70% đối với đại thực bào. ApoAI được tìm thấy trong môi trường nuôi cấy đại thực bào, chủ yếu liên quan đến phân đoạn HDL. Điều thú vị là người ta đã tìm thấy sự gia tăng đáng kể về mức mRNA và protein đối với ATP liên kết với cassette A1 (ABCA1) và ABCG1 trong các đại thực bào biểu hiện apoAI sau khi tải acLDL. Biểu hiện của apoAI làm tăng đáng kể lượng cholesterol thoát ra ở các đại thực bào kiểu hoang dã và apoE(-/-). Các HPC được chuyển đổi bằng LV-apoAI ngoài cơ thể và sau đó được cấy ghép vào chuột apoE(-/-) đã làm giảm 50% diện tích tổn thương xơ vữa động mạch so với nhóm đối chứng GFP, mà không ảnh hưởng đến nồng độ HDL-C trong huyết tương. KẾT LUẬN Chuyển đổi lentivirus apoAI thành HPC làm giảm xơ vữa động mạch ở chuột apoE(-/-). Biểu hiện của apoAI trong các đại thực bào cải thiện quá trình vận chuyển cholesterol trong các đại thực bào sản xuất apoE kiểu hoang dã và gây ra sự điều chỉnh tăng ABCA1 và ABCG1. Những quan sát mới này thiết lập giai đoạn cho một phương pháp điều trị bằng tế bào để hồi phục xơ vữa động mạch, khai thác sự hợp tác giữa apoE và apoAI để tối đa hóa sự thoát cholesterol khỏi mảng bám."} +{"idx": 1970096, "en": "Stem or progenitor cell-based strategies to combat ischemic heart disease and myocardial infarction, whether autologous transplantation or stimulation of resident populations, not only require detailed insight into transdifferentiation potential and functional coupling, but the efficacy of this approach is underpinned by the need to induce appropriate migration and homing to the site of injury. This review focuses on existing insights into the trafficking of stem cells in the context of cardiac regenerative therapy, with particular focus on the wide variety of potential sources of cells, critical factors that may regulate their migration, and how extrapolating from embryonic stem/progenitor cell behavior during cardiogenesis may reveal pathways implicit in the adult heart postinjury.", "gemini": "Các chiến lược dựa trên tế bào gốc hoặc tế bào tiền thân để chống lại bệnh tim thiếu máu cục bộ và nhồi máu cơ tim, cho dù là ghép tự thân hay kích thích quần thể trú ngụ, không chỉ đòi hỏi hiểu biết chi tiết về tiềm năng biệt hóa chéo và sự kết hợp chức năng, mà hiệu quả của phương pháp này còn được củng cố bởi nhu cầu gây ra sự di chuyển thích hợp và định vị đến vị trí tổn thương. Đánh giá này tập trung vào những hiểu biết hiện có về quá trình vận chuyển của các tế bào gốc trong bối cảnh liệu pháp tái tạo tim, đặc biệt tập trung vào nhiều nguồn tế bào tiềm năng, các yếu tố quan trọng có thể điều chỉnh quá trình di chuyển của chúng và cách ngoại suy từ hành vi của tế bào gốc/tiền thân phôi trong quá trình tạo tim có thể tiết lộ các con đường ngụ ý trong tim người trưởng thành sau chấn thương."} +{"idx": 1970097, "en": "The Notch signaling pathway has been demonstrated to play a critical role during mammalian cardiac development based on recent findings from gene-targeted mice. In addition, mutations in the Notch signaling pathway have been associated with human congenital heart defects such as Alagille syndrome, bicuspid aortic valve disease, calcification of the heart valves, and ventricular septal defects. Recently, it was demonstrated that Notch activation in the endocardium regulates ventricular myocardial development and that the Notch downstream target genes Hey1 and Hey2 are required for the establishment of the atrioventricular canal myocardial boundary. The Notch pathway has previously been implicated in regulating endothelial-to-mesenchymal transition during development of the heart valves, and recent reports further dissect the role of individual Notch downstream target genes during this process. In addition, a role for the Notch pathway during cardiac neural crest cell development has been identified, which provides a potential mechanism for the findings seen in Alagille syndrome. This review focuses on recently reported findings that elucidate mechanisms regulated by the Notch pathway during ventricular, atrioventricular canal, and outflow tract development.", "gemini": "Con đường truyền tín hiệu Notch đã được chứng minh đóng vai trò quan trọng trong quá trình phát triển tim ở động vật có vú dựa trên những phát hiện gần đây từ chuột được nhắm mục tiêu gen. Ngoài ra, các đột biến trong con đường truyền tín hiệu Notch có liên quan đến các khuyết tật tim bẩm sinh ở người như hội chứng Alagille, bệnh van động mạch chủ hai lá, vôi hóa van tim và các khuyết tật vách liên thất. Gần đây, người ta đã chứng minh rằng sự hoạt hóa Notch ở nội mạc điều chỉnh sự phát triển cơ tim thất và các gen đích hạ nguồn Notch là Hey1 và Hey2 là cần thiết để thiết lập ranh giới cơ tim của ống nhĩ thất. Con đường Notch trước đây đã được chứng minh là có liên quan đến việc điều chỉnh quá trình chuyển đổi nội mô thành trung mô trong quá trình phát triển van tim và các báo cáo gần đây tiếp tục phân tích vai trò của từng gen đích hạ nguồn Notch trong quá trình này. Ngoài ra, một vai trò của con đường Notch trong quá trình phát triển tế bào mào thần kinh tim đã được xác định, cung cấp một cơ chế tiềm năng cho những phát hiện thấy được trong hội chứng Alagille. Đánh giá này tập trung vào những phát hiện được báo cáo gần đây làm sáng tỏ các cơ chế được điều chỉnh bởi con đường Notch trong quá trình phát triển thất, ống nhĩ thất và đường thoát."} +{"idx": 1970098, "en": "POEMS syndrome is a rare plasma cell disorder characterized by peripheral neuropathy, monoclonal gammopathy, and high levels of serum vascular endothelial growth factor, the pathogenesis of which remains unclear. A unique feature of this syndrome is that the proliferating monoclonal plasma cells are essentially lambda-restricted. Here we determined complete nucleotide sequences of monoclonal immunoglobulin lambda light chain (IGL) variable regions in 11 patients with POEMS syndrome. The V-region of the Ig lambda gene of all 11 patients was restricted to the V lambda 1 subfamily. Searching for homologies with IGL germlines revealed that 2 germlines, IGLV1-44*01 (9/11) and IGLV1-40*01 (2/10), were identified, with an average homology of 91.1%. The IGLJ3*02 gene was used in 11 of 11 re-arrangements with an average homology of 92.2%. These data suggest that the highly restricted use of IGL V lambda 1 germlines plays an important role in the pathogenesis of POEMS syndrome.", "gemini": "Hội chứng POEMS là một rối loạn tế bào plasma hiếm gặp đặc trưng bởi bệnh thần kinh ngoại biên, bệnh lý globulin đơn dòng và nồng độ cao yếu tố tăng trưởng nội mô mạch máu trong huyết thanh, cơ chế sinh bệnh của hội chứng này vẫn chưa rõ ràng. Một đặc điểm riêng biệt của hội chứng này là các tế bào plasma đơn dòng tăng sinh về cơ bản bị hạn chế lambda. Ở đây chúng tôi xác định trình tự nucleotide hoàn chỉnh của chuỗi biến đổi nhẹ immunoglobulin lambda đơn dòng (IGL) ở 11 bệnh nhân mắc hội chứng POEMS. Vùng V của gen Ig lambda của tất cả 11 bệnh nhân đều bị hạn chế ở phân họ V lambda 1. Tìm kiếm sự đồng đẳng với các phôi IGL cho thấy 2 phôi, IGLV1-44*01 (9/11) và IGLV1-40*01 (2/10), đã được xác định, với mức đồng đẳng trung bình là 91,1%. Gen IGLJ3*02 được sử dụng trong 11/11 lần sắp xếp lại với mức đồng đẳng trung bình là 92,2%. Những dữ liệu này cho thấy việc sử dụng rất hạn chế các phôi IGL V lambda 1 đóng vai trò quan trọng trong cơ chế sinh bệnh của hội chứng POEMS."} +{"idx": 1970099, "en": "B-cell chronic lymphocytic leukemia (B-CLL) is a lymphoproliferative disorder characterized by the surface expression of CD20, CD5 antigens, as well as the receptor CD40. Activation of CD40 by its ligand (CD40L) induces proliferation and rescues the cells from spontaneous and chemotherapy-induced apoptosis. CD40 activation also induces secretion of cytokines, such as IL-6, IL-10, TNF-alpha, IL-8, and GM-CSF, which are involved in tumor cell survival, migration, and interaction with cells in the tumor microenvironment. Here we demonstrate that in primary B-CLL tumor cells, the novel antagonist anti-CD40 monoclonal antibody, HCD122, inhibits CD40L-induced activation of signaling pathways, proliferation and survival, and secretion of cytokines. Furthermore, HCD122 is also a potent mediator of antibody-dependent cellular cytotoxicity (ADCC), lysing B-CLL cells more efficiently than rituximab in vitro, despite a significantly higher number of cell surface CD20 binding sites compared with CD40. Unlike rituximab, however, HCD122 (formerly CHIR-12.12) does not internalize upon binding to the cells. Our data suggest that HCD122 may inhibit B-CLL growth by blocking CD40 signaling and by ADCC-mediated cell lysis.", "gemini": "Bệnh bạch cầu lymphocytic mãn tính tế bào B (B-CLL) là một rối loạn tăng sinh lympho đặc trưng bởi sự biểu hiện trên bề mặt của các kháng nguyên CD20, CD5, cũng như thụ thể CD40. Sự hoạt hóa của CD40 bởi phối tử của nó (CD40L) gây ra sự tăng sinh và giải cứu các tế bào khỏi quá trình apoptosis tự phát và do hóa trị liệu gây ra. Sự hoạt hóa của CD40 cũng gây ra sự tiết ra các cytokine, chẳng hạn như IL-6, IL-10, TNF-alpha, IL-8 và GM-CSF, có liên quan đến sự sống còn của tế bào khối u, di chuyển và tương tác với các tế bào trong vi môi trường khối u. Ở đây chúng tôi chứng minh rằng trong các tế bào khối u B-CLL nguyên phát, kháng thể đơn dòng kháng CD40 mới lạ, HCD122, ức chế sự hoạt hóa của các con đường truyền tín hiệu do CD40L gây ra, sự tăng sinh và sống sót, và sự tiết ra các cytokine. Hơn nữa, HCD122 cũng là một chất trung gian mạnh mẽ của tế bào độc tế bào phụ thuộc vào kháng thể (ADCC), tiêu hủy các tế bào B-CLL hiệu quả hơn rituximab trong ống nghiệm, mặc dù có số lượng vị trí liên kết CD20 trên bề mặt tế bào cao hơn đáng kể so với CD40. Tuy nhiên, không giống như rituximab, HCD122 (trước đây là CHIR-12.12) không nội tại hóa khi liên kết với các tế bào. Dữ liệu của chúng tôi cho thấy HCD122 có thể ức chế sự phát triển của B-CLL bằng cách chặn tín hiệu CD40 và bằng quá trình phân giải tế bào do ADCC."} +{"idx": 1970100, "en": "Residual vein thrombosis (RVT) indicates a prothrombotic state and is useful for evaluating the optimal duration of oral anticoagulant treatment (OAT). Patients with a first episode of deep vein thrombosis, treated with OAT for 3 months, were managed according to RVT findings. Those with RVT were randomized to either stop or continue anticoagulants for 9 additional months, whereas in those without RVT, OAT was stopped. Outcomes were recurrent venous thromboembolism and/or major bleeding. Residual thrombosis was detected in 180 (69.8%) of 258 patients; recurrent events occurred in 27.2% of those who discontinued (25/92; 15.2% person-years) and 19.3% of those who continued OAT (17/88; 10.1% person-years). The relative adjusted hazard ratio (HR) was 1.58 (95% confidence interval [CI], 0.85-2.93; P = .145). Of the 78 (30.2%) patients without RVT, only 1 (1.3%; 0.63% person-years) had a recurrence. The adjusted HR of patients with RVT versus those without was 24.9 (95% CI, 3.4-183.6; P = .002). One major bleeding event (1.1%; 0.53% person-years) occurred in patients who stopped and 2 occurred (2.3%; 1.1% person-years) in those who continued OAT. Absence of RVT identifies a group of patients at very low risk for recurrent thrombosis who can safely stop OAT. This trial was registered at http://www.ClinicalTrials.gov as no. NCT00438230.", "gemini": "Thuyên tắc tĩnh mạch còn lại (RVT) chỉ ra tình trạng tăng đông và hữu ích để đánh giá thời gian điều trị thuốc chống đông đường uống (OAT) tối ưu. Những bệnh nhân bị huyết khối tĩnh mạch sâu lần đầu, được điều trị bằng OAT trong 3 tháng, được quản lý theo các phát hiện của RVT. Những bệnh nhân bị RVT được chỉ định ngẫu nhiên ngừng hoặc tiếp tục dùng thuốc chống đông trong 9 tháng tiếp theo, trong khi ở những bệnh nhân không bị RVT, OAT đã bị dừng lại. Kết quả là huyết khối tắc mạch tĩnh mạch tái phát và/hoặc chảy máu nghiêm trọng. Thuyên tắc còn lại được phát hiện ở 180 (69,8%) trong số 258 bệnh nhân; các biến cố tái phát xảy ra ở 27,2% những người ngừng thuốc (25/92; 15,2% người-năm) và 19,3% những người tiếp tục dùng OAT (17/88; 10,1% người-năm). Tỷ lệ nguy cơ điều chỉnh tương đối (HR) là 1,58 (khoảng tin cậy 95% [CI], 0,85-2,93; P = .145). Trong số 78 (30,2%) bệnh nhân không bị RVT, chỉ có 1 (1,3%; 0,63% người-năm) bị tái phát. HR được điều chỉnh của những bệnh nhân bị RVT so với những bệnh nhân không bị RVT là 24,9 (95% CI, 3,4-183,6; P = .002). Một biến cố chảy máu nghiêm trọng (1,1%; 0,53% người-năm) xảy ra ở những bệnh nhân ngừng thuốc và 2 biến cố xảy ra (2,3%; 1,1% người-năm) ở những bệnh nhân tiếp tục dùng OAT. Việc không có RVT xác định một nhóm bệnh nhân có nguy cơ tái phát huyết khối rất thấp, những người có thể ngừng OAT một cách an toàn. Thử nghiệm này đã được đăng ký tại http://www.ClinicalTrials.gov với số. NCT00438230."} +{"idx": 1970101, "en": "We evaluated the impact of World Health Organization (WHO) classification and WHO classification-based Prognostic Scoring System (WPSS) on the outcome of patients with myelodysplastic syndrome (MDS) who underwent allogeneic stem cell transplantation (allo-SCT) between 1990 and 2006. Five-year overall survival (OS) was 80% in refractory anemias, 57% in refractory cytopenias, 51% in refractory anemia with excess blasts 1 (RAEB-1), 28% in RAEB-2, and 25% in acute leukemia from MDS (P = .001). Five-year probability of relapse was 9%, 22%, 24%, 56%, and 53%, respectively (P < .001). Five-year transplant-related mortality (TRM) was 14%, 39%, 38%, 34%, and 44%, respectively (P = .24). In multivariate analysis, WHO classification showed a significant effect on OS (P = .017) and probability of relapse (P = .01); transfusion dependency was associated with a reduced OS (P = .01) and increased TRM (P = .037), whereas WPSS showed a prognostic significance on both OS (P = .001) and probability of relapse (P < .001). In patients without excess blasts, multilineage dysplasia and transfusion dependency affected OS (P = .001 and P = .009, respectively), and were associated with an increased TRM (P = .013 and P = .031, respectively). In these patients, WPSS identified 2 groups with different OS and TRM. These data suggest that WHO classification and WPSS have a relevant prognostic value in posttransplantation outcome of MDS patients.", "gemini": "Chúng tôi đã đánh giá tác động của phân loại Tổ chức Y tế Thế giới (WHO) và Hệ thống chấm điểm tiên lượng dựa trên phân loại WHO (WPSS) đối với kết cục của những bệnh nhân mắc hội chứng loạn sản tủy (MDS) đã trải qua ghép tế bào gốc đồng loại (allo-SCT) từ năm 1990 đến 2006. Tỷ lệ sống chung sau 5 năm (OS) là 80% ở tình trạng thiếu máu khó chữa, 57% ở tình trạng giảm tế bào máu khó chữa, 51% ở tình trạng thiếu máu khó chữa với lượng nổ thừa 1 (RAEB-1), 28% ở RAEB-2 và 25% ở bệnh bạch cầu cấp tính từ MDS (P = .001). Xác suất tái phát sau 5 năm lần lượt là 9%, 22%, 24%, 56% và 53% (P < .001). Tỷ lệ tử vong liên quan đến ghép sau 5 năm (TRM) lần lượt là 14%, 39%, 38%, 34% và 44% (P = .24). Trong phân tích đa biến, phân loại của WHO cho thấy tác động đáng kể đến OS (P = .017) và khả năng tái phát (P = .01); sự phụ thuộc vào truyền máu có liên quan đến việc giảm OS (P = .01) và tăng TRM (P = .037), trong khi WPSS cho thấy ý nghĩa tiên lượng trên cả OS (P = .001) và khả năng tái phát (P < .001). Ở những bệnh nhân không có lượng nổ thừa, loạn sản đa dòng và phụ thuộc vào truyền máu ảnh hưởng đến OS (P = .001 và P = .009) và có liên quan đến việc tăng TRM (P = .013 và P = .031). Ở những bệnh nhân này, WPSS xác định 2 nhóm có OS và TRM khác nhau. Những dữ liệu này cho thấy phân loại của WHO và WPSS có giá trị tiên lượng có liên quan đến kết cục sau ghép của bệnh nhân MDS."} +{"idx": 1970102, "en": "Glioblastoma multiforme (GBM) is the most frequent primary brain tumor in adults. Prognosis is poor. Using a series of 214 GBM patients, we observed an effect of the variant 5,10-methylenetetrahydrofolate reductase (MTHFR) c.677C>T on overall survival. This effect was strongest in patients younger than 60 years at diagnosis (overall survival, median +/- SE: genotype CC, 13 +/- 1 months; CT, 11 +/- 2 months; TT, 7 +/- 3 months; multivariate Cox regression analysis, Wald = 8.58, p = 0.007). In addition, the MTHFR genotype significantly influenced the overall survival of patients with a postoperative Karnofsky score >70 (CC, 12 +/- 2 months; CT, 11 +/- 1 months; TT, 10 +/- 4 months; Wald = 5.89, p = 0.015). These data suggest the MTHFR c.677C>T variant is a risk factor for survival in GBM patients.", "gemini": "U nguyên bào đa hình dạng sao (GBM) là khối u não nguyên phát thường gặp nhất ở người lớn. Tiên lượng kém. Sử dụng một loạt 214 bệnh nhân GBM, chúng tôi quan sát thấy tác dụng của biến thể 5,10-methylenetetrahydrofolate reductase (MTHFR) c.677C>T đối với sự sống sót chung. Tác dụng này mạnh nhất ở những bệnh nhân dưới 60 tuổi khi chẩn đoán (sống sót chung, trung bình +/- SE: kiểu gen CC, 13 +/- 1 tháng; CT, 11 +/- 2 tháng; TT, 7 +/- 3 tháng; phân tích hồi quy Cox đa biến, Wald = 8,58, p = 0,007). Ngoài ra, kiểu gen MTHFR ảnh hưởng đáng kể đến sự sống sót chung của những bệnh nhân có điểm Karnofsky sau phẫu thuật >70 (CC, 12 +/- 2 tháng; CT, 11 +/- 1 tháng; TT, 10 +/- 4 tháng; Wald = 5,89, p = 0,015). Những dữ liệu này cho thấy biến thể MTHFR c.677C>T là một yếu tố nguy cơ đối với sự sống sót ở những bệnh nhân GBM."} +{"idx": 1970103, "en": "A phase I trial was conducted to determine the maximum tolerated dose (MTD) of temozolomide given in combination with lomustine in newly diagnosed pediatric patients with high-grade gliomas. Response was assessed following two courses of therapy at the MTD. Temozolomide was administered to cohorts of patients at doses of 100, 125, 160, or 200 mg/m(2) on days 1-5, along with 90 mg/m(2) lomustine on day 1. Two courses of lomustine/temozolomide were given prior to radiation therapy (RT) and up to six courses were administered afterward. Thirty-two patients were enrolled. Dose-limiting myelosuppression was seen in two of three patients enrolled at the 200 mg/m(2) dose level. One of 14 patients in the expanded MTD cohort (160 mg/m(2)) experienced dose-limiting thrombocytopenia. After two courses at the MTD, one patient with a 5-mm enhancing nodule postoperatively had a complete response, one patient with a large residual temporal lobe glioblastoma had a partial response, and eight patients had stable disease. Several patients developed transient radiographic worsening after completing RT. Median 1- and 2-year overall survivals at the MTD were 60% +/- 13% and 40% +/- 13% with a median of 17.6 months. Thirteen of 20 patients (65%) who underwent MRI scans within 6 months prior to death developed metastatic disease. In conclusion, when administered with 90 mg/m(2) lomustine on day 1, the MTD of temozolomide is 160 mg/m(2)/day x 5. Radiographic changes following RT make determination of early tumor progression difficult. Metastatic disease is common prior to death.", "gemini": "Một thử nghiệm giai đoạn I đã được tiến hành để xác định liều tối đa dung nạp (MTD) của temozolomide dùng kết hợp với lomustine ở những bệnh nhi mới được chẩn đoán mắc u thần kinh đệm cấp độ cao. Đáp ứng được đánh giá sau hai đợt điều trị ở MTD. Temozolomide được dùng cho các nhóm bệnh nhân với liều 100, 125, 160 hoặc 200 mg/m(2) vào các ngày 1-5, cùng với 90 mg/m(2) lomustine vào ngày 1. Hai đợt lomustine/temozolomide được dùng trước xạ trị (RT) và tối đa sáu đợt được dùng sau đó. Ba mươi hai bệnh nhân được ghi danh. Ức chế tủy có giới hạn liều được thấy ở hai trong số ba bệnh nhân được ghi danh ở mức liều 200 mg/m(2). Một trong số 14 bệnh nhân trong nhóm MTD mở rộng (160 mg/m(2)) bị giảm tiểu cầu có giới hạn liều. Sau hai đợt ở MTD, một bệnh nhân có nốt tăng cường 5 mm sau phẫu thuật có đáp ứng hoàn toàn, một bệnh nhân có u nguyên bào thần kinh đệm thùy thái dương còn sót lại lớn có đáp ứng một phần và tám bệnh nhân có bệnh ổn định. Một số bệnh nhân phát triển tình trạng xấu đi thoáng qua trên phim chụp X quang sau khi hoàn thành RT. Tỷ lệ sống sót chung sau 1 và 2 năm ở MTD lần lượt là 60% +/- 13% và 40% +/- 13% với thời gian sống trung bình là 17,6 tháng. Mười ba trong số 20 bệnh nhân (65%) đã chụp MRI trong vòng 6 tháng trước khi tử vong phát triển bệnh di căn. Tóm lại, khi dùng với 90 mg/m(2) lomustine vào ngày 1, MTD của temozolomide là 160 mg/m(2)/ngày x 5. Những thay đổi trên phim chụp X quang sau RT khiến việc xác định tiến triển khối u sớm trở nên khó khăn. Bệnh di căn thường gặp trước khi tử vong."} +{"idx": 1970104, "en": "Vascular smooth muscle cell (VSMC) accumulation is implicated in plaque development. In contrast, VSMC apoptosis is implicated in plaque rupture, coagulation, vessel remodeling, medial atrophy, aneurysm formation, and calcification. Although VSMC apoptosis accompanies multiple pathologies, there is little proof of direct causality, particularly with the low levels of VSMC apoptosis seen in vivo. Using a mouse model of inducible VSMC-specific apoptosis, we demonstrate that low-level VSMC apoptosis during either atherogenesis or within established plaques of apolipoprotein (Apo)E(-/-) mice accelerates plaque growth by two-fold, associated with features of plaque vulnerability including a thin fibrous cap and expanded necrotic core. Chronic VSMC apoptosis induced development of calcified plaques in younger animals and promoted calcification within established plaques. In addition, VSMC apoptosis induced medial expansion, associated with increased elastic lamina breaks, and abnormal matrix deposition reminiscent of cystic medial necrosis in humans. VSMC apoptosis prevented outward remodeling associated with atherosclerosis resulting in marked vessel stenosis. We conclude that VSMC apoptosis is sufficient to accelerate atherosclerosis, promote plaque calcification and medial degeneration, prevent expansive remodeling, and promote stenosis in atherosclerosis.", "gemini": "Sự tích tụ của tế bào cơ trơn mạch máu (VSMC) liên quan đến sự phát triển mảng bám. Ngược lại, quá trình apoptosis của VSMC liên quan đến vỡ mảng bám, đông máu, tái tạo mạch máu, teo lớp giữa, hình thành phình động mạch và vôi hóa. Mặc dù quá trình apoptosis của VSMC đi kèm với nhiều bệnh lý, nhưng có ít bằng chứng về nguyên nhân trực tiếp, đặc biệt là với mức độ apoptosis của VSMC thấp được thấy trong cơ thể sống. Sử dụng mô hình chuột apoptosis đặc hiệu của VSMC có thể gây ra, chúng tôi chứng minh rằng quá trình apoptosis của VSMC ở mức độ thấp trong quá trình hình thành xơ vữa động mạch hoặc trong các mảng bám đã được thiết lập ở những con chuột apolipoprotein (Apo)E(-/-) làm tăng gấp đôi sự phát triển của mảng bám, liên quan đến các đặc điểm của mảng bám dễ bị tổn thương bao gồm lớp xơ mỏng và nhân hoại tử mở rộng. Quá trình apoptosis mạn tính của VSMC gây ra sự phát triển của các mảng bám vôi hóa ở những động vật trẻ hơn và thúc đẩy sự vôi hóa trong các mảng bám đã được thiết lập. Ngoài ra, quá trình apoptosis của VSMC gây ra sự giãn nở lớp giữa, liên quan đến việc tăng vỡ lớp đàn hồi và lắng đọng ma trận bất thường gợi nhớ đến hoại tử lớp giữa dạng nang ở người. Quá trình apoptosis của VSMC ngăn ngừa sự tái tạo hướng ra ngoài liên quan đến xơ vữa động mạch dẫn đến hẹp mạch rõ rệt. Chúng tôi kết luận rằng quá trình apoptosis của VSMC là đủ để đẩy nhanh quá trình xơ vữa động mạch, thúc đẩy sự vôi hóa của mảng bám và thoái hóa lớp giữa, ngăn ngừa sự tái tạo mở rộng và thúc đẩy hẹp trong xơ vữa động mạch."} +{"idx": 1970105, "en": "Platelet hyperactivity associated with hyperlipidemia may contribute to development of a prothrombotic state. We previously showed that oxidized low-density lipoprotein (oxLDL) formed in the setting of hyperlipidemia and atherosclerosis activated platelets in a CD36-dependent manner. We now show that mitogen-activated protein kinase c-Jun N-terminal kinase (JNK)2 and its upstream activator MKK4 were phosphorylated in platelets exposed to oxLDL. Using apoE(-/-) mice as a model of hyperlipidemia, we showed that JNK was constitutively phosphorylated in platelets in a CD36-dependent manner. Inhibition of src kinase activity reduced JNK phosphorylation by oxLDL. Immunoprecipitations revealed that active phosphorylated forms of src kinases Fyn and Lyn were recruited to CD36 in platelets exposed to oxLDL. Pharmacological inhibition of the mitogen-activated protein kinase JNK or src family kinases abolished platelet activation by oxLDL in vitro. Using a murine carotid artery thrombosis model we demonstrated CD36-dependent phosphorylation of platelet JNK within thrombi. Furthermore, pharmacological inhibition of JNK prolonged thrombosis times in wild-type but not cd36-null mice in vivo. These findings suggest that a specific CD36-dependent signaling pathway is required for platelet activation by oxLDL and may provide insights related to development of novel antiplatelet therapies more relevant to atherothrombosis than to normal hemostasis.", "gemini": "Tăng hoạt tính tiểu cầu liên quan đến tăng lipid máu có thể góp phần vào sự phát triển của tình trạng huyết khối. Trước đây, chúng tôi đã chỉ ra rằng lipoprotein tỷ trọng thấp bị oxy hóa (oxLDL) được hình thành trong bối cảnh tăng lipid máu và xơ vữa động mạch làm hoạt hóa tiểu cầu theo cách phụ thuộc CD36. Bây giờ chúng tôi chỉ ra rằng protein kinase hoạt hóa mitogen c-Jun N-terminal kinase (JNK) 2 và chất hoạt hóa thượng nguồn của nó là MKK4 đã được phosphoryl hóa ở tiểu cầu tiếp xúc với oxLDL. Sử dụng chuột apoE(-/-) làm mô hình tăng lipid máu, chúng tôi đã chỉ ra rằng JNK được phosphoryl hóa liên tục ở tiểu cầu theo cách phụ thuộc CD36. Ức chế hoạt tính của src kinase làm giảm quá trình phosphoryl hóa JNK bởi oxLDL. Kết tủa miễn dịch cho thấy các dạng phosphoryl hóa hoạt động của src kinase Fyn và Lyn được tuyển dụng đến CD36 ở tiểu cầu tiếp xúc với oxLDL. Ức chế dược lý protein kinase hoạt hóa mitogen JNK hoặc src kinase làm mất hoạt động tiểu cầu do oxLDL trong ống nghiệm. Sử dụng mô hình huyết khối động mạch cảnh ở chuột, chúng tôi đã chứng minh quá trình phosphoryl hóa JNK tiểu cầu phụ thuộc CD36 trong huyết khối. Hơn nữa, ức chế dược lý JNK kéo dài thời gian huyết khối ở chuột kiểu hoang dã nhưng không phải ở chuột thiếu cd36 trong cơ thể sống. Những phát hiện này cho thấy một con đường truyền tín hiệu phụ thuộc CD36 cụ thể là cần thiết cho hoạt động tiểu cầu do oxLDL và có thể cung cấp thông tin chi tiết liên quan đến sự phát triển các liệu pháp chống tiểu cầu mới có liên quan nhiều hơn đến huyết khối động mạch hơn là cầm máu bình thường."} +{"idx": 1970106, "en": "Leupaxin is a LIM domain-containing adapter protein belonging to the paxillin family that has been previously reported to be preferentially expressed in hematopoietic cells. Herein, we identified leupaxin in a screen for focal adhesion kinase binding partners in aortic smooth muscle, and we show that leupaxin is enriched in human and mouse vascular smooth muscle and that leupaxin expression is dynamically regulated during development. In addition, our studies reveal that leupaxin can undergo cytoplasmic/nuclear shuttling and functions as an serum response factor cofactor in the nucleus. We found that leupaxin forms a complex with serum response factor and associates with CArG-containing regions of smooth muscle promoters and that ectopic expression of leupaxin induces smooth muscle marker gene expression in both 10T1/2 cells and rat aortic smooth muscle cells. Subsequent studies indicated that enhanced focal adhesion kinase activity (induced by fibronectin or expression of constitutively active focal adhesion kinase) attenuates the nuclear accumulation of leupaxin and limits the ability of leupaxin to enhance serum response factor-dependent gene transcription. Thus, these studies indicate that modulation of the subcellular localization of serum response factor cofactors is 1 mechanism by which extracellular matrix-dependent signals may regulate phenotypic switching of smooth muscle cells.", "gemini": "Leupaxin là một protein thích ứng chứa miền LIM thuộc họ paxillin đã được báo cáo trước đây là được biểu hiện ưu tiên trong các tế bào tạo máu. Ở đây, chúng tôi đã xác định leupaxin trong quá trình sàng lọc các đối tác liên kết với kinase gắn kết tiêu điểm ở cơ trơn động mạch chủ và chúng tôi chỉ ra rằng leupaxin được làm giàu trong cơ trơn mạch máu ở người và chuột và biểu hiện leupaxin được điều hòa động trong quá trình phát triển. Ngoài ra, các nghiên cứu của chúng tôi cho thấy leupaxin có thể trải qua sự vận chuyển tế bào chất/nhân và hoạt động như một yếu tố đồng yếu tố đáp ứng huyết thanh trong nhân. Chúng tôi thấy rằng leupaxin tạo thành một phức hợp với yếu tố đáp ứng huyết thanh và liên kết với các vùng chứa CArG của các chất thúc đẩy cơ trơn và biểu hiện leupaxin lạc chỗ gây ra biểu hiện gen đánh dấu cơ trơn ở cả tế bào 10T1/2 và tế bào cơ trơn động mạch chủ của chuột. Các nghiên cứu tiếp theo chỉ ra rằng hoạt động kinase gắn kết tiêu điểm tăng cường (do fibronectin hoặc biểu hiện kinase gắn kết tiêu điểm hoạt động liên tục gây ra) làm giảm sự tích tụ leupaxin trong nhân và hạn chế khả năng tăng cường phiên mã gen phụ thuộc yếu tố đáp ứng huyết thanh của leupaxin. Do đó, các nghiên cứu này chỉ ra rằng điều chế vị trí dưới tế bào của các yếu tố đồng yếu tố đáp ứng huyết thanh là 1 cơ chế mà các tín hiệu phụ thuộc ma trận ngoại bào có thể điều chỉnh sự chuyển đổi kiểu hình của các tế bào cơ trơn."} +{"idx": 1970107, "en": "Mice deficient in the organic solute transporter (Ost)-alpha subunit of the heteromeric organic solute and steroid transporter, Ostalpha-Ostbeta, were generated and were found to be viable and fertile but exhibited small intestinal hypertrophy and growth retardation. Bile acid pool size and serum levels were decreased by more than 60% in Ostalpha-/- mice, whereas fecal bile acid excretion was unchanged, suggesting a defect in intestinal bile acid absorption. In support of this hypothesis, when [3H]taurocholic acid or [3H]estrone 3-sulfate were administered into the ileal lumen, absorption was lower in Ostalpha-/- mice. Interestingly, serum cholesterol and triglyceride levels were also approximately 15% lower in Ostalpha-/- mice, an effect that may be related to the impaired intestinal bile acid absorption. After intraperitoneal administration of [3H]estrone 3-sulfate or [3H]dehydroepiandrosterone sulfate, Ostalpha-/- mice had higher levels of radioactivity in their liver and urinary bladder and less in the duodenum, indicating altered hepatic, renal, and intestinal disposition. Loss of Ostalpha was associated with compensatory changes in the expression of several genes involved in bile acid homeostasis, including an increase in the multidrug resistance-associated protein 3, (Mrp3)/Abcc3, an alternate basolateral bile acid export pump, and a decrease in cholesterol 7alpha-hydroxylase, Cyp7a1, the rate-limiting enzyme in bile acid synthesis. The latter finding may be explained by increased ileal expression of fibroblast growth factor 15 (Fgf15), a negative regulator of hepatic Cyp7a1 transcription. Overall, these findings provide direct support for the hypothesis that Ostalpha-Ostbeta is a major basolateral transporter of bile acids and conjugated steroids in the intestine, kidney, and liver.", "gemini": "Chuột thiếu tiểu đơn vị alpha của chất vận chuyển chất hữu cơ (Ost), Ostalpha-Ostbeta, của chất vận chuyển steroid và chất hữu cơ dị hợp, Ostalpha-Ostbeta, đã được tạo ra và được phát hiện là khả thi và sinh sản nhưng biểu hiện chứng phì đại ruột non và chậm phát triển. Kích thước bể axit mật và nồng độ huyết thanh giảm hơn 60% ở chuột Ostalpha-/- trong khi bài tiết axit mật trong phân không thay đổi, điều này cho thấy sự khiếm khuyết trong quá trình hấp thụ axit mật ở ruột. Để hỗ trợ cho giả thuyết này, khi axit [3H]taurocholic hoặc [3H]estrone 3-sulfate được đưa vào lòng hồi tràng, sự hấp thụ ở chuột Ostalpha-/- thấp hơn. Điều thú vị là nồng độ cholesterol và triglyceride trong huyết thanh cũng thấp hơn khoảng 15% ở chuột Ostalpha-/-, một tác dụng có thể liên quan đến sự hấp thụ axit mật ở ruột bị suy yếu. Sau khi tiêm phúc mạc [3H]estrone 3-sulfate hoặc [3H]dehydroepiandrosterone sulfate, chuột Ostalpha-/- có nồng độ phóng xạ cao hơn ở gan và bàng quang và ít hơn ở tá tràng, cho thấy sự thay đổi về tình trạng gan, thận và ruột. Sự mất Ostalpha liên quan đến những thay đổi bù trừ trong biểu hiện của một số gen liên quan đến cân bằng axit mật, bao gồm sự gia tăng protein liên quan đến kháng đa thuốc 3 (Mrp3)/Abcc3, một bơm xuất axit mật đáy bên thay thế và sự giảm hydroxylase cholesterol 7alpha, Cyp7a1, là enzyme giới hạn tốc độ trong quá trình tổng hợp axit mật. Phát hiện sau này có thể được giải thích bằng sự gia tăng biểu hiện yếu tố tăng trưởng nguyên bào sợi 15 (Fgf15) ở hồi tràng, một chất điều hòa tiêu cực của phiên mã Cyp7a1 ở gan. Nhìn chung, những phát hiện này cung cấp bằng chứng trực tiếp cho giả thuyết rằng Ostalpha-Ostbeta là một chất vận chuyển đáy bên chính của axit mật và steroid liên hợp ở ruột, thận và gan."} +{"idx": 1970108, "en": "In cirrhosis, hepatic venous pressure gradient is used to measure portal venous and sinusoidal pressures, as well as drug-induced decreases of elevated pressures. The aim of this study was to investigate the influence of hepatic arterial flow (HAF) changes on portal venous perfusion (PVPP) and wedged hepatic venous pressure (WHVP). Normal and CCl4-cirrhotic rats were subjected to a bivascular liver perfusion with continuous measurements of PVPP, WHVP, and hepatic arterial perfusion pressure. Flow-pressure curves were performed with the use of different flows either through the portal vein (PVF: 20-32 ml/min) or HAF (5-15 ml/min). Increases in HAF lead to significant absolute and relative increases in PVPP (P = 0.002) and WHVP (P < 0.001). Absolute changes in HAF correlated to absolute changes in PVPP (cirrhosis: r = 0.64, P < 0.001; control: r = 0.67, P < 0.001) and WHVP (cirrhosis: r = 0.71, P < 0.001; control: r = 0.82, P < 0.001). Changes in PVPP correlated to changes in WHVP due to changes in PVF only in cirrhosis (r = 0.75, P < 0.001), whereas changes in HAF correlated in both cirrhosis (r = 0.92, P < 0.001) and control (r = 0.77, P < 0.001). In conclusion, increases and decreases in HAF lead to respective changes in PVPP and WHVP. This suggests a direct influence of HAF on PVPP and WHVP most likely due to changes in sinusoidal perfusion.", "gemini": "Trong xơ gan, độ chênh áp tĩnh mạch gan được sử dụng để đo áp lực tĩnh mạch cửa và áp lực xoang, cũng như sự giảm áp lực tăng do thuốc gây ra. Mục đích của nghiên cứu này là điều tra ảnh hưởng của những thay đổi về lưu lượng động mạch gan (HAF) đến tưới máu tĩnh mạch cửa (PVPP) và áp lực tĩnh mạch gan nêm (WHVP). Chuột bình thường và chuột xơ gan CCl4 được tiến hành tưới máu gan hai mạch với các phép đo liên tục về PVPP, WHVP và áp lực tưới máu động mạch gan. Các đường cong lưu lượng-áp lực được thực hiện bằng cách sử dụng các lưu lượng khác nhau qua tĩnh mạch cửa (PVF: 20-32 ml/phút) hoặc HAF (5-15 ml/phút). Sự gia tăng HAF dẫn đến sự gia tăng tuyệt đối và tương đối đáng kể về PVPP (P = 0,002) và WHVP (P < 0,001). Những thay đổi tuyệt đối về HAF tương quan với những thay đổi tuyệt đối về PVPP (xơ gan: r = 0,64, P < 0,001; đối chứng: r = 0,67, P < 0,001) và WHVP (xơ gan: r = 0,71, P < 0,001; đối chứng: r = 0,82, P < 0,001). Những thay đổi về PVPP tương quan với những thay đổi về WHVP do những thay đổi về PVF chỉ ở xơ gan (r = 0,75, P < 0,001), trong khi những thay đổi về HAF tương quan ở cả xơ gan (r = 0,92, P < 0,001) và đối chứng (r = 0,77, P < 0,001). Tóm lại, sự gia tăng và giảm HAF dẫn đến những thay đổi tương ứng về PVPP và WHVP. Điều này gợi ý rằng HAF có ảnh hưởng trực tiếp đến PVPP và WHVP rất có thể là do những thay đổi về tưới máu xoang."} +{"idx": 1970109, "en": "Heme oxygenase overexpression or exogenous carbon monoxide (CO) protects against hepatocyte apoptosis and fulminant hepatitis. The prevention of hepatocyte apoptosis by CO has been shown to require activation of NF-kappaB. The purpose of these investigations was to determine the mechanism of CO-induced hepatocyte NF-kappaB activation and protection against apoptosis. Primary rat or mouse hepatocytes and Hep3B cells were utilized. CO exposure was performed at 250 parts per million. Main outcome measures included cell viability, reactive oxygen species (ROS) generation, and changes in the levels of the intracellular antioxidants glutathione and ascorbate. Western blotting was performed for phospho-Akt, total Akt, and IkappaBalpha. NF-kappaB activation was determined by electrophoretic mobility shift assay and luciferase reporter assays. We found that CO treatment of hepatocytes prevents spontaneous apoptosis and leads to an increase in ROS production in association with Akt phosphorylation and IkappaB degradation. CO did not increase ROS production in respiration-deficient (rho0) Hep3B cells. Both Akt phosphorylation and IkappaB degradation can be inhibited by the addition of antioxidants. Furthermore, CO-induced NF-kappaB activation is reversed by phosphatidylinositol 3-kinase (PI3-K) inhibitor (LY294002) or antioxidants. Additionally, prevention of spontaneous hepatocyte apoptosis by CO is reversed by PI3-K inhibition and antioxidants. In conclusion, these data implicate a survival pathway of CO-induced ROS, Akt phosphorylation, and NF-kappaB activation in cultured hepatocytes. This pathway may prove to be important in maintenance of hepatic function in both physiological and pathophysiological conditions.", "gemini": "Sự biểu hiện quá mức của heme oxygenase hoặc carbon monoxide ngoại sinh (CO) bảo vệ tế bào gan khỏi chết theo chương trình và viêm gan tối cấp. Việc ngăn ngừa tế bào gan chết theo chương trình bằng CO đã được chứng minh là đòi hỏi phải kích hoạt NF-kappaB. Mục đích của các cuộc điều tra này là xác định cơ chế hoạt hóa NF-kappaB của tế bào gan do CO gây ra và bảo vệ chống lại quá trình chết theo chương trình. Tế bào gan chính của chuột hoặc chuột và tế bào Hep3B đã được sử dụng. Phơi nhiễm CO được thực hiện ở mức 250 phần triệu. Các biện pháp kết quả chính bao gồm khả năng sống của tế bào, tạo ra các loài oxy phản ứng (ROS) và những thay đổi về nồng độ của các chất chống oxy hóa nội bào glutathione và ascorbate. Điện di Western được thực hiện đối với phospho-Akt, Akt toàn phần và IkappaBalpha. Hoạt hóa NF-kappaB được xác định bằng phương pháp dịch chuyển độ linh động điện di và phương pháp thử nghiệm luciferase. Chúng tôi phát hiện ra rằng xử lý CO đối với tế bào gan ngăn ngừa quá trình chết theo chương trình tự phát và dẫn đến sự gia tăng sản xuất ROS liên quan đến quá trình phosphoryl hóa Akt và thoái hóa IkappaB. CO không làm tăng sản xuất ROS trong các tế bào Hep3B thiếu hô hấp (rho0). Cả quá trình phosphoryl hóa Akt và thoái hóa IkappaB đều có thể bị ức chế bằng cách bổ sung chất chống oxy hóa. Hơn nữa, hoạt hóa NF-kappaB do CO gây ra bị đảo ngược bởi chất ức chế phosphatidylinositol 3-kinase (PI3-K) (LY294002) hoặc chất chống oxy hóa. Ngoài ra, việc ngăn ngừa tế bào gan chết theo chương trình tự phát bằng CO bị đảo ngược bởi chất ức chế PI3-K và chất chống oxy hóa. Tóm lại, những dữ liệu này liên quan đến một con đường sống sót của ROS do CO gây ra, quá trình phosphoryl hóa Akt và hoạt hóa NF-kappaB trong tế bào gan nuôi cấy. Con đường này có thể chứng tỏ là quan trọng trong việc duy trì chức năng gan trong cả các tình trạng sinh lý và bệnh lý."} +{"idx": 1970110, "en": "To study the regulation of fenestrations by vascular endothelial growth factor in liver sinusoidal endothelial cells, SK Hep1 cells were transfected with green fluorescence protein (GFP)-actin and GFP-caveolin-1. SK Hep1 cells had pores; some of which appeared to be fenestrations (diameter 55 +/- 28 nm, porosity 2.0 +/- 1.4%), rudimentary sieve plates, bristle-coated micropinocytotic vesicles and expressed caveolin-1, von Willebrand factor, vascular endothelial growth factor receptor-2, endothelial nitric oxide synthase and clathrin, but not CD31. There was avid uptake of formaldehyde serum albumin, consistent with endocytosis. Vascular endothelial growth factor caused an increase in porosity to 4.8 +/- 2.6% (P < 0.01) and pore diameter to 104 +/- 59 nm (P < 0.001). GFP-actin was expressed throughout the cells, whereas GFP-caveolin-1 had a punctate appearance; both responded to vascular endothelial growth factor by contraction toward the nucleus over hours in parallel with the formation of fenestrations. SK Hep1 cells resemble liver sinusoidal endothelial cells, and the vascular endothelial growth factor-induced formation of fenestration-like pores is preceded by contraction of actin cytoskeleton and attached caveolin-1 toward the nucleus.", "gemini": "Để nghiên cứu sự điều hòa của các lỗ hổng do yếu tố tăng trưởng nội mạc mạch máu ở các tế bào nội mạc xoang gan, các tế bào SK Hep1 được chuyển gen với protein huỳnh quang xanh (GFP)-actin và GFP-caveolin-1. Các tế bào SK Hep1 có lỗ chân lông; một số trong số chúng dường như là lỗ hổng (đường kính 55 +/- 28 nm, độ xốp 2,0 +/- 1,4%), các tấm sàng thô sơ, các túi vi nang micropinocytotic phủ lông và biểu hiện caveolin-1, yếu tố von Willebrand, thụ thể yếu tố tăng trưởng nội mạc mạch máu-2, nitric oxit synthase nội mạc và clathrin, nhưng không phải CD31. Có sự hấp thụ mạnh mẽ huyết thanh albumin formaldehyde, phù hợp với quá trình nội bào. Yếu tố tăng trưởng nội mạc mạch máu gây ra sự gia tăng độ xốp lên 4,8 +/- 2,6% (P < 0,01) và đường kính lỗ lên 104 +/- 59 nm (P < 0,001). GFP-actin được biểu hiện trong toàn bộ tế bào, trong khi GFP-caveolin-1 có dạng chấm; cả hai đều phản ứng với yếu tố tăng trưởng nội mạc mạch máu bằng cách co lại về phía nhân trong nhiều giờ song song với sự hình thành các lỗ hổng. Các tế bào SK Hep1 giống với các tế bào nội mạc xoang gan, và sự hình thành các lỗ giống như lỗ hổng do yếu tố tăng trưởng nội mạc mạch máu gây ra trước đó là sự co lại của bộ xương tế bào actin và caveolin-1 gắn vào về phía nhân."} +{"idx": 1970111, "en": "Hypospadias is one of the most common congenital anomalies in the United States, occurring in approximately 1 in 125 live male births. Embryological studies have demonstrated that, depending on where the urethral development arrests, the meatal opening can be anywhere along the shaft of the penis or, in more severe forms, within the scrotum or in the perineum. Currently, the only available treatment is surgery. If left uncorrected, especially in its severe form, there is risk of infertility and psychological effects, such as avoidance of intimate relationships. The cause of hypospadias is largely unknown; however, current epidemiology and laboratory studies have shed new light into the etiology of hypospadias. With recent advancements in molecular biology and microarray technology, it appears that hypospadias is potentially related to disrupted gene expression. Specifically, some of the environmental chemicals are acting as antiandrogens and interfere directly with the action of testosterone-related gene expression. In this paper, we briefly review the normal development of male external genitalia and the prevalence and environmental risk factors related to hypospadias. In addition, we discuss some of the recent laboratory findings that contribute to our current understanding of this disease.", "gemini": "Thiểu sản dương vật là một trong những dị tật bẩm sinh phổ biến nhất ở Hoa Kỳ, xảy ra ở khoảng 1/125 trẻ em trai được sinh ra. Các nghiên cứu phôi thai đã chứng minh rằng, tùy thuộc vào vị trí phát triển của niệu đạo bị dừng lại, lỗ mở niệu đạo có thể nằm ở bất kỳ vị trí nào dọc theo trục của dương vật hoặc ở dạng nghiêm trọng hơn, nằm trong bìu hoặc ở tầng sinh môn. Hiện tại, phương pháp điều trị duy nhất có sẵn là phẫu thuật. Nếu không được điều chỉnh, đặc biệt là ở dạng nghiêm trọng, có nguy cơ vô sinh và các tác động về mặt tâm lý, chẳng hạn như tránh các mối quan hệ thân mật. Nguyên nhân gây ra thiểu sản dương vật phần lớn vẫn chưa được biết; tuy nhiên, các nghiên cứu dịch tễ học và phòng thí nghiệm hiện nay đã làm sáng tỏ nguyên nhân gây ra thiểu sản dương vật. Với những tiến bộ gần đây trong công nghệ sinh học phân tử và mảng vi mô, có vẻ như thiểu sản dương vật có khả năng liên quan đến sự gián đoạn biểu hiện gen. Cụ thể, một số hóa chất trong môi trường hoạt động như chất kháng androgen và can thiệp trực tiếp vào hoạt động biểu hiện gen liên quan đến testosterone. Trong bài báo này, chúng tôi xem xét tóm tắt quá trình phát triển bình thường của bộ phận sinh dục ngoài của nam giới và tỷ lệ mắc bệnh cũng như các yếu tố nguy cơ liên quan đến môi trường đối với thiểu sản dương vật. Ngoài ra, chúng tôi thảo luận về một số phát hiện gần đây trong phòng thí nghiệm góp phần vào sự hiểu biết hiện tại của chúng tôi về căn bệnh này."} +{"idx": 1970112, "en": "Understanding the dynamics of spermatogenesis is central to clinical andrology or to probing environmental effects on human testes. This review considers what is known about renewal and proliferation of spermatogonia, how germ cells are organized in cellular associations constituting the cycle of the seminiferous epithelium, relative frequencies of cellular associations, durations of the cycle of the seminiferous epithelium and spermatogenesis, and measurement of daily sperm production. Daily sperm production (DSP) per testis tends to decline with advancing age. Regardless of age, there is substantial loss of potential sperm from degeneration of spermatocytes, but not spermatids. DSP per gram testis parenchyma or DSP per testis cannot be predicted on the basis of testis size or age of a man. The review shows why our 1960s data base is neither robust nor precise and suggests how deficiencies might be rectified. New cellular associations should be defined, with none representing >15% of the cycle of the seminiferous epithelium. Then determine when A(pale)-spermatogonia become committed to proliferate or how many mitotic divisions occur thereafter. Restudy the duration of spermatogenesis because the accepted value might be in error by approximately 6 days. Restudying human spermatogenesis will benefit clinicians, toxicologists, and epidemiologists probing testis function by direct evaluations or indirectly via evaluations of quantity and quality of sperm ejaculated. It also will benefit scientists interested in renewal and proliferation of spermatogonia, or a spermatogonium as a prototype stem cell.", "gemini": "Hiểu được động lực của quá trình sinh tinh là trọng tâm của nam khoa lâm sàng hoặc để thăm dò các tác động của môi trường lên tinh hoàn của con người. Đánh giá này xem xét những gì được biết về sự tái tạo và tăng sinh của tinh nguyên bào, cách các tế bào mầm được tổ chức trong các liên kết tế bào cấu thành chu kỳ của biểu mô ống sinh tinh, tần suất tương đối của các liên kết tế bào, thời gian của chu kỳ biểu mô ống sinh tinh và quá trình sinh tinh, và phép đo sản xuất tinh trùng hàng ngày. Sản lượng tinh trùng hàng ngày (DSP) trên mỗi tinh hoàn có xu hướng giảm theo tuổi tác. Bất kể tuổi tác, vẫn có sự mất mát đáng kể tinh trùng tiềm ẩn do thoái hóa của tế bào tinh bào, nhưng không phải tế bào tinh trùng. Không thể dự đoán DSP trên mỗi gam nhu mô tinh hoàn hoặc DSP trên mỗi tinh hoàn dựa trên kích thước tinh hoàn hoặc tuổi của nam giới. Đánh giá cho thấy tại sao cơ sở dữ liệu những năm 1960 của chúng tôi không mạnh cũng không chính xác và gợi ý cách khắc phục những thiếu sót. Nên xác định các liên kết tế bào mới, không có liên kết nào chiếm > 15% chu kỳ biểu mô ống sinh tinh. Sau đó xác định thời điểm tinh nguyên bào A (nhạt) bắt đầu tăng sinh hoặc có bao nhiêu lần phân chia nguyên phân xảy ra sau đó. Nghiên cứu lại thời gian sinh tinh vì giá trị được chấp nhận có thể sai lệch khoảng 6 ngày. Nghiên cứu lại quá trình sinh tinh ở người sẽ có lợi cho các bác sĩ lâm sàng, nhà độc học và nhà dịch tễ học thăm dò chức năng tinh hoàn bằng cách đánh giá trực tiếp hoặc gián tiếp thông qua việc đánh giá số lượng và chất lượng tinh trùng xuất tinh. Nó cũng sẽ có lợi cho các nhà khoa học quan tâm đến sự tái tạo và tăng sinh của tinh nguyên bào, hoặc tinh nguyên bào như một tế bào gốc nguyên mẫu."} +{"idx": 1970113, "en": "Progesterone induces a fast transient calcium influx in human sperm though the activation of nongenomic receptors. During sperm capacitation, a complex process required for sperm to be able to fertilize the egg, the calcium influx induced by progesterone is enhanced. Sperm capacitation is mediated by an increase in cAMP content and subsequent protein kinase A (PKA) activation. In this work, we examined the effect of increasing intracellular cAMP on the calcium influx induced by progesterone in noncapacitated human sperm. To do this, sperm were exposed to the phosphodiesterase inhibitor papaverine for 5 minutes, a treatment that increased both the cAMP content and the PKA activity several-fold. The calcium influx induced by progesterone was increased by papaverine to levels close to those found in capacitated sperm. This effect was partially inhibited by H89 (48%) and by genistein (41%), and the sum of both inhibitors reduced the stimulating effect of papaverine by 89%. The inhibitory effect of genistein on the progesterone-induced calcium influx could be related to its capability to inhibit the papaverine-stimulated increase in cAMP content and PKA activity. The results presented here suggest that the calcium influx induced by progesterone is up-regulated by the PKA activity.", "gemini": "Progesterone gây ra dòng canxi thoáng qua nhanh ở tinh trùng người mặc dù sự hoạt hóa của các thụ thể không liên quan đến bộ gen. Trong quá trình trưởng thành của tinh trùng, một quá trình phức tạp cần thiết để tinh trùng có thể thụ tinh cho trứng, dòng canxi do progesterone gây ra được tăng cường. Quá trình trưởng thành của tinh trùng được trung gian bởi sự gia tăng hàm lượng cAMP và hoạt hóa protein kinase A (PKA) sau đó. Trong nghiên cứu này, chúng tôi đã kiểm tra tác dụng của việc tăng cAMP nội bào đối với dòng canxi do progesterone gây ra ở tinh trùng người chưa trưởng thành. Để làm được điều này, tinh trùng đã được tiếp xúc với chất ức chế phosphodiesterase papaverine trong 5 phút, một phương pháp điều trị làm tăng cả hàm lượng cAMP và hoạt động của PKA gấp nhiều lần. Dòng canxi do progesterone gây ra đã được papaverine tăng lên đến mức gần bằng mức tìm thấy ở tinh trùng đã trưởng thành. Hiệu ứng này bị ức chế một phần bởi H89 (48%) và genistein (41%), và tổng của cả hai chất ức chế này làm giảm hiệu ứng kích thích của papaverine xuống 89%. Tác dụng ức chế của genistein đối với dòng canxi do progesterone gây ra có thể liên quan đến khả năng ức chế sự gia tăng hàm lượng cAMP và hoạt động của PKA do papaverine kích thích. Các kết quả trình bày ở đây cho thấy rằng dòng canxi do progesterone gây ra được điều hòa tăng lên bởi hoạt động của PKA."} +{"idx": 1970114, "en": "The UBE2B gene encodes ubiquitin-conjugating enzyme, which is involved in DNA repair. Ube2b knockout mice were found to be infertile because of structural abnormality of sperm. However, there is no genetic study on the role of the UBE2B gene in human fertility; therefore, the present investigation was designed to study genetic variations in the UBE2B gene and its role in human male infertility. Sequence analyses of the UBE2B gene in 530 infertile (350 azoospermic, 105 oligoasthenoteratozoospermic, and 75 oligoasthenozoospermic) and 300 fertile control men revealed the presence of 5 substitution single-nucleotide polymorphisms (SNPs) in 221 individuals (199 infertile [37.5%] and 22 fertile [7.3%] men). Of these, 2 (g.5197:T>G; g.9157:A>G) of the 5 substitutions were novel and observed only in infertile men. Distribution of haplotypes TA, TG, GA, and GG are not uniform between the patient and the control group of this study. Interestingly, our study suggests that the haplotype TG conferred significantly increased risk for male infertility (odds ratio = 5.07, 95% CI = 1.29-23.29, p = .007). In silico analysis of SNPs that were specific to infertile men predicted that these SNPs lead to defective splicing by destroying or creating the potential binding site of splicing factors or causing alteration in predicted regulatory sequences. In the light of the above, our study suggests that the UBE2B gene is associated with male infertility in Indian men, hence, providing evidence for additional genetic factors for male infertility.", "gemini": "Gen UBE2B mã hóa enzyme liên hợp ubiquitin, có liên quan đến sửa chữa DNA. Chuột bị loại gen Ube2b được phát hiện là vô sinh do bất thường về cấu trúc tinh trùng. Tuy nhiên, không có nghiên cứu di truyền nào về vai trò của gen UBE2B trong khả năng sinh sản của con người; do đó, cuộc điều tra hiện tại được thiết kế để nghiên cứu các biến thể di truyền trong gen UBE2B và vai trò của nó trong tình trạng vô sinh ở nam giới. Phân tích trình tự của gen UBE2B ở 530 nam giới vô sinh (350 bị vô tinh trùng, 105 bị thiểu năng tinh trùng và 75 bị thiểu năng tinh trùng) và 300 nam giới có khả năng sinh sản cho thấy sự hiện diện của 5 đa hình một nucleotide thay thế (SNP) ở 221 cá thể (199 vô sinh [37,5%] và 22 có khả năng sinh sản [7,3%] nam giới). Trong số này, 2 (g.5197: T> G; g.9157: A> G) trong số 5 lần thay thế là mới và chỉ được quan sát thấy ở những người đàn ông vô sinh. Sự phân bố của các kiểu gen TA, TG, GA và GG không thống nhất giữa nhóm bệnh nhân và nhóm đối chứng trong nghiên cứu này. Điều thú vị là nghiên cứu của chúng tôi cho thấy kiểu gen TG làm tăng đáng kể nguy cơ vô sinh ở nam giới (tỷ lệ chênh lệch = 5,07, CI 95% = 1,29-23,29, p = 0,007). Phân tích in silico của các SNP đặc hiệu đối với những người đàn ông vô sinh dự đoán rằng các SNP này dẫn đến sự ghép nối khiếm khuyết bằng cách phá hủy hoặc tạo ra vị trí liên kết tiềm năng của các yếu tố ghép nối hoặc gây ra sự thay đổi trong các trình tự điều hòa được dự đoán. Theo quan điểm trên, nghiên cứu của chúng tôi cho thấy rằng gen UBE2B có liên quan đến tình trạng vô sinh ở nam giới Ấn Độ, do đó, cung cấp bằng chứng về các yếu tố di truyền bổ sung cho tình trạng vô sinh ở nam giới."} +{"idx": 1970115, "en": "To address the role of PKC isoforms in hyperglycemia-induced apoptosis and malformations in the embryos of diabetic pregnancies, expression of PKCalpha, beta1, beta 2, gamma, delta, epsilon, and zeta was examined in the neural tube of rat embryos and showed to overlap with the regions of increased apoptosis. Levels of activated (phosphorylated) PKCalpha , beta2, and delta were increased in the embryos of diabetic dams whereas those of PKCepsilon and zeta were decreased when compared with those in control groups. Cytosolic phospholipase A(2) (cPLA(2)) was also activated. Blocking the activity of PKCalpha , beta2, and delta using isoform-specific inhibitors in embryos cultured in hyperglycemia (40 mM) reduced malformation rates when compared with those in untreated hyperglycemic and euglycemic (8.3 mM) groups. These observations demonstrate that PKCalpha, beta2, and delta play an essential role in diabetic embryopathy. Activation of cPLA(2) was also decreased, suggesting that PKCs mediate the hyperglycemic effects through the cPLA(2)-phospholipid peroxidation pathway.", "gemini": "Để giải quyết vai trò của các đồng dạng PKC trong quá trình apoptosis do tăng đường huyết và dị tật ở phôi của thai kỳ tiểu đường, biểu hiện của PKCalpha, beta1, beta 2, gamma, delta, epsilon và zeta đã được kiểm tra trong ống thần kinh của phôi chuột và cho thấy chồng chéo với các vùng tăng apoptosis. Nồng độ PKCalpha, beta2 và delta hoạt động (bị phosphoryl hóa) tăng lên ở phôi của những con chuột mẹ bị tiểu đường trong khi nồng độ PKCepsilon và zeta giảm khi so sánh với những con trong nhóm đối chứng. Phospholipase A (2) tế bào chất (cPLA (2)) cũng được hoạt hóa. Việc chặn hoạt động của PKCalpha, beta2 và delta bằng cách sử dụng các chất ức chế đặc hiệu đồng dạng ở phôi được nuôi cấy trong tình trạng tăng đường huyết (40 mM) làm giảm tỷ lệ dị tật khi so sánh với những nhóm tăng đường huyết không được điều trị và đường huyết bình thường (8,3 mM). Những quan sát này chứng minh rằng PKCalpha, beta2 và delta đóng vai trò thiết yếu trong bệnh lý phôi thai do tiểu đường. Sự hoạt hóa của cPLA (2) cũng giảm, cho thấy rằng PKC trung gian các tác động tăng đường huyết thông qua con đường peroxy hóa lipid cPLA (2)."} +{"idx": 1970116, "en": "Pregnancy is a physiological state that involves a significant decrease in uterine vascular tone and an increase in uterine blood flow, which is mediated in part by steroid hormones, including estrogen, progesterone, and cortisol. Previous studies have demonstrated the involvement of these hormones in the regulation of uterine artery contractility through signaling pathways specific to the endothelium and the vascular smooth muscle. Alterations in endothelial nitric oxide synthase expression and activity, nitric oxide production, and expression of enzymes involved in PGI(2) production contribute to the uterine artery endothelium-specific responses. Steroid hormones also have an effect on calcium-activated potassium channel activity, PKC signaling pathway and myogenic tone, and alterations in pharmacomechanical coupling in the uterine artery smooth muscle. This review addresses current understanding of the molecular mechanisms by which steroid hormones including estrogen, progesterone, and cortisol modulate uterine artery contractility to alter uterine blood flow during pregnancy with an emphasis on the pregnant ewe model.", "gemini": "Mang thai là trạng thái sinh lý liên quan đến sự giảm đáng kể trương lực mạch tử cung và tăng lưu lượng máu tử cung, một phần do các hormone steroid, bao gồm estrogen, progesterone và cortisol, trung gian. Các nghiên cứu trước đây đã chứng minh sự tham gia của các hormone này trong quá trình điều hòa khả năng co bóp của động mạch tử cung thông qua các con đường truyền tín hiệu đặc hiệu đối với nội mạc và cơ trơn mạch máu. Sự thay đổi trong biểu hiện và hoạt động của nitric oxide synthase nội mạc, sản xuất nitric oxide và biểu hiện của các enzyme liên quan đến sản xuất PGI (2) góp phần vào các phản ứng đặc hiệu của nội mạc động mạch tử cung. Các hormone steroid cũng có tác dụng lên hoạt động của kênh kali được canxi kích hoạt, con đường truyền tín hiệu PKC và trương lực cơ và sự thay đổi trong sự ghép nối dược cơ ở cơ trơn động mạch tử cung. Đánh giá này đề cập đến sự hiểu biết hiện tại về các cơ chế phân tử mà các hormone steroid bao gồm estrogen, progesterone và cortisol điều chỉnh khả năng co bóp của động mạch tử cung để thay đổi lưu lượng máu tử cung trong thời kỳ mang thai với sự nhấn mạnh vào mô hình cừu mang thai."} +{"idx": 1970117, "en": "The aim of this prospective paired cohort study is to elucidate the impact of ovarian stimulation with recombinant follicle-stimulating hormone in combination with gonadotropin-releasing hormone antagonist on the endometrial transcriptome. Oocyte donors underwent endometrial biopsy during the implantation window of the nonstimulated cycle and following ovarian stimulation with recombinant follicle-stimulating hormone and gonadotropin-releasing hormone antagonist but no luteal progesterone supplementation (n = 4). Microarray analysis showed 142 genes to be significantly upregulated and 98 significantly downregulated. Significantly upregulated genes included those sequencing for the chemokine ligand CXCL 13, the Dickkopf homolog, steroidogenic acute regulatory protein, and homeobox C6. Also upregulated were genes inhibited by progesterone, such as insulin-like growth factor binding protein 5. In conclusion, ovarian stimulation with follicle-stimulating hormone and gonadotropin-releasing hormone antagonist dysregulates the expression of many genes involved in cell adhesion, T-cell receptor signaling, and regulation of signal transduction. These data suggest that dysregulation of the endometrial transcriptome in the stimulated cycle is not fully attributable to supraphysiological sex steroid levels at the folliculo-luteal transition.", "gemini": "Mục tiêu của nghiên cứu theo dõi theo cặp triển vọng này là làm sáng tỏ tác động của kích thích buồng trứng bằng hormone kích thích nang tái tổ hợp kết hợp với chất đối kháng hormone giải phóng gonadotropin lên bản sao mã nội mạc tử cung. Những người hiến noãn đã được sinh thiết nội mạc tử cung trong thời gian làm tổ của chu kỳ không được kích thích và sau khi kích thích buồng trứng bằng hormone kích thích nang tái tổ hợp và chất đối kháng hormone giải phóng gonadotropin nhưng không bổ sung progesterone hoàng thể (n = 4). Phân tích mảng gen cho thấy 142 gen được điều hòa tăng lên đáng kể và 98 gen được điều hòa giảm đáng kể. Các gen được điều hòa tăng lên đáng kể bao gồm các gen giải trình tự cho phối tử chemokine CXCL 13, đồng đẳng Dickkopf, protein điều hòa cấp tính steroidogenic và homeobox C6. Các gen bị ức chế bởi progesterone, chẳng hạn như protein liên kết yếu tố tăng trưởng giống insulin 5, cũng được điều hòa tăng lên. Tóm lại, kích thích buồng trứng bằng hormone kích thích nang và chất đối kháng hormone giải phóng gonadotropin làm mất điều hòa biểu hiện của nhiều gen liên quan đến sự kết dính tế bào, tín hiệu thụ thể tế bào T và điều hòa truyền tín hiệu. Những dữ liệu này cho thấy sự mất điều hòa của bản sao mã nội mạc tử cung trong chu kỳ được kích thích không hoàn toàn là do nồng độ steroid sinh dục trên mức sinh lý ở giai đoạn chuyển tiếp nang hoàng thể."} +{"idx": 1970118, "en": "The regulatory mechanisms underlying myometrial smooth muscle contractility during labor are poorly understood. The authors therefore investigated the transcriptional profile of the changes that occur in the human myometrium at term pregnancy when compared with that at labor. Microarray technology was used to identify differentially expressed genes in human myometrium at labor. Real-time fluorescence reversetranscriptase polymerase chain reaction (RT-PCR) was subsequently performed to verify the microarray data. Semiquantitative RT-PCR, Western blotting, and microscopy methodologies were also used. Certain novel genes were found to be upregulated in human myometrium at labor. Of these, PSCDBP, TLR2, TWIST1 , FLJ35382, andRGS12 have not been previously characterized or identified in human myometrium. EDNRB is the other novel labor-associated gene whose reported expression is also upregulated at labor. All 6 genes were expressed on human myometrial smooth muscle cells. These novel upregulated genes are involved in multiple pathways that may be associated with a variety of cellular processes including inflammation, transcriptional regulation, and intracellular signaling.", "gemini": "Các cơ chế điều hòa cơ sở cho khả năng co cơ trơn tử cung trong quá trình chuyển dạ vẫn chưa được hiểu rõ. Do đó, các tác giả đã nghiên cứu hồ sơ phiên mã của những thay đổi xảy ra ở cơ tử cung của người ở thai kỳ đủ tháng khi so sánh với thai kỳ chuyển dạ. Công nghệ mảng gen đã được sử dụng để xác định các gen biểu hiện khác biệt ở cơ tử cung của người trong quá trình chuyển dạ. Sau đó, phản ứng chuỗi polymerase phiên mã ngược huỳnh quang thời gian thực (RT-PCR) được thực hiện để xác minh dữ liệu mảng gen. Các phương pháp RT-PCR bán định lượng, nhuộm màu Western và kính hiển vi cũng được sử dụng. Một số gen mới đã được phát hiện là được điều hòa tăng lên ở cơ tử cung của người trong quá trình chuyển dạ. Trong số đó, PSCDBP, TLR2, TWIST1, FLJ35382 và RGS12 chưa được đặc trưng hoặc xác định trước đây ở cơ tử cung của người. EDNRB là một gen mới liên quan đến chuyển dạ khác có biểu hiện được báo cáo cũng được điều hòa tăng lên trong quá trình chuyển dạ. Cả 6 gen đều được biểu hiện trên các tế bào cơ trơn tử cung của người. Các gen mới được điều hòa tăng lên này có liên quan đến nhiều con đường có thể liên quan đến nhiều quá trình của tế bào bao gồm viêm, điều hòa phiên mã và truyền tín hiệu nội bào."} +{"idx": 1970119, "en": "The objective of this study is to describe the effects of oral mifepristone administration on saliva levels of estradiol, estriol, progesterone, and cortisol in women with postdates pregnancy. As an adjunct to a randomized controlled trial comparing 200 mg oral mifepristone to placebo for cervical ripening and labor induction in women with pregnancies greater than 41 weeks' gestation, saliva samples were obtained before drug administration and every 6 hours thereafter for 24 hours. Estradiol, estriol, progesterone, and cortisol levels were measured by radioimmunoassay. Ninety-seven participants received mifepristone, and 83 received placebo. Saliva steroid hormone data were available for 71 mifepristone-and 60 placebo-treated women. Mean baseline saliva estradiol, estriol, progesterone, and cortisol levels were similar between study groups. At 24 hours after study medication administration, saliva estradiol, estriol, progesterone, and cortisol levels in the mifepristone group were significantly elevated compared with baseline. There was no significant change in hormone levels in the placebo group. Oral mifepristone significantly increased saliva estradiol, estriol, progesterone, and cortisol compared with placebo. This may reflect mifepristone's antiglucocorticoid properties. These hormone elevations may contribute to the mechanism by which mifepristone causes cervical ripening and increases myometrial activity.", "gemini": "Mục tiêu của nghiên cứu này là mô tả tác dụng của việc dùng mifepristone đường uống lên nồng độ estradiol, estriol, progesterone và cortisol trong nước bọt ở những phụ nữ mang thai quá ngày. Như một phần bổ sung cho một thử nghiệm đối chứng ngẫu nhiên so sánh mifepristone uống 200 mg với giả dược để làm chín cổ tử cung và gây chuyển dạ ở những phụ nữ mang thai trên 41 tuần, các mẫu nước bọt đã được lấy trước khi dùng thuốc và cứ 6 giờ sau đó trong 24 giờ. Nồng độ estradiol, estriol, progesterone và cortisol được đo bằng phương pháp miễn dịch phóng xạ. Chín mươi bảy người tham gia đã dùng mifepristone và 83 người dùng giả dược. Dữ liệu về hormone steroid trong nước bọt có sẵn cho 71 phụ nữ dùng mifepristone và 60 phụ nữ dùng giả dược. Nồng độ estradiol, estriol, progesterone và cortisol trung bình ban đầu trong nước bọt tương tự nhau giữa các nhóm nghiên cứu. Sau 24 giờ dùng thuốc nghiên cứu, nồng độ estradiol, estriol, progesterone và cortisol trong nước bọt ở nhóm dùng mifepristone tăng đáng kể so với ban đầu. Không có sự thay đổi đáng kể nào về nồng độ hormone ở nhóm dùng giả dược. Mifepristone đường uống làm tăng đáng kể nồng độ estradiol, estriol, progesterone và cortisol trong nước bọt so với giả dược. Điều này có thể phản ánh đặc tính chống glucocorticoid của mifepristone. Sự gia tăng hormone này có thể góp phần vào cơ chế mà mifepristone gây ra sự chín của cổ tử cung và làm tăng hoạt động của tử cung."} +{"idx": 1970120, "en": "Observations from the authors' laboratory suggest a physiological role for increased cyclin G1 protein levels in human uterine leiomyoma. The hypothesis of the present study is that the strategic modulation of cyclin G1 by antisense technology will inhibit the survival of in vitro-grown uterine leiomyoma cells. Cultured uterine leiomyoma cells were transfected with cyclin G1 ribbon-type antisense oligonucleotide (cyclin G1 RiAS) to effectively reduce cyclin G1 expression. Cell viability, in situ terminal deoxyuridine nick end-labeling (TUNEL) assay, flow cytometry, DNA fragmentation, and expression of cell cycle regulatory-related proteins were evaluated by Western blot. Antisense oligonucleotides compromised uterine leiomyoma cell viability and inducted apoptosis in a caspase-independent mechanism. In situ TUNEL and DNA fragmentation revealed apoptosis induction, and fluorescent-activated cell sorting analysis showed increased sub-G1-phase cells. Furthermore, abrogation of cyclin G1 enhanced p53 accumulation, phosphorylation of p53 at Ser-15 residue, and increased expression of cyclin-dependent kinase inhibitors p21 and p27. These data imply that cyclin G1 expression is associated with growth promotion and the potential utility and novelty of using ribbon-type antisense oligonucleotides as a gene therapy strategy to treat human uterine leiomyoma.", "gemini": "Các quan sát từ phòng thí nghiệm của các tác giả cho thấy một vai trò sinh lý đối với mức protein cyclin G1 tăng trong u cơ tử cung ở người. Giả thuyết của nghiên cứu hiện tại là sự điều chỉnh chiến lược cyclin G1 bằng công nghệ antisense sẽ ức chế sự sống sót của các tế bào u cơ tử cung được nuôi trong ống nghiệm. Các tế bào u cơ tử cung được nuôi cấy đã được chuyển nạp oligonucleotide antisense loại ruy băng cyclin G1 (cyclin G1 RiAS) để giảm hiệu quả biểu hiện cyclin G1. Độ sống của tế bào, xét nghiệm đánh dấu tận cùng deoxyuridine tại chỗ (TUNEL), đo lưu lượng tế bào, phân mảnh DNA và biểu hiện của các protein liên quan đến điều hòa chu kỳ tế bào đã được đánh giá bằng Western blot. Các oligonucleotide antisense làm giảm độ sống của tế bào u cơ tử cung và gây apoptosis theo cơ chế không phụ thuộc caspase. TUNEL tại chỗ và phân mảnh DNA cho thấy quá trình gây apoptosis, và phân tích phân loại tế bào hoạt hóa huỳnh quang cho thấy sự gia tăng các tế bào pha dưới G1. Hơn nữa, việc loại bỏ cyclin G1 làm tăng sự tích tụ p53, quá trình phosphoryl hóa p53 tại gốc Ser-15 và tăng biểu hiện của các chất ức chế kinase phụ thuộc cyclin p21 và p27. Những dữ liệu này ngụ ý rằng biểu hiện cyclin G1 có liên quan đến việc thúc đẩy tăng trưởng và tính hữu ích tiềm tàng và tính mới của việc sử dụng oligonucleotide antisense loại ruy băng làm chiến lược điều trị gen để điều trị u cơ tử cung ở người."} +{"idx": 1970121, "en": "The authors investigated the influence of pregnancy and gender on the density of trigeminal and sympathetic perivascular nerves in posterior cerebral arteries (PCA) and the reactivity to norepinephrine and calcitonin gene-related peptide (CGRP). PCAs were isolated from nonpregnant, late-pregnant, postpartum, and male rats, mounted and pressurized on an arteriograph chamber to obtain concentration-response curves to norepinephrine and CGRP. Arteries were immunostained for CGRP-, tyrosine hydroxylase-, and protein gene product 9.5 (PGP 9.5)-containing perivascular nerves, and nerve density was determined morphologically. Pregnancy had a trophic effect on trigeminal perivascular innervation (P < .01 vs male); however, this was not accompanied by a change in reactivity to CGRP. Sympathetic and PGP 9.5 nerve densities were not altered by pregnancy or gender, and there were no differences in reactivity to norepinephrine. Together, these results suggest that the increase in trigeminal innervation during pregnancy is more related to nociception than in controlling resting cerebral blood flow.", "gemini": "Các tác giả đã nghiên cứu ảnh hưởng của thai kỳ và giới tính đến mật độ của các dây thần kinh quanh mạch máu giao cảm và dây sinh ba ở động mạch não sau (PCA) và khả năng phản ứng với norepinephrine và peptide liên quan đến gen calcitonin (CGRP). Các PCA được lấy từ những con chuột không mang thai, mang thai muộn, sau sinh và chuột đực, được gắn và tạo áp lực trên buồng động mạch để có được các đường cong đáp ứng nồng độ với norepinephrine và CGRP. Các động mạch được nhuộm miễn dịch đối với các dây thần kinh quanh mạch máu chứa CGRP-, tyrosine hydroxylase- và sản phẩm gen protein 9,5 (PGP 9,5) và mật độ dây thần kinh được xác định về mặt hình thái. Thai kỳ có tác dụng dinh dưỡng đối với sự chi phối quanh mạch máu của dây sinh ba (P < 0,01 so với nam); tuy nhiên, điều này không đi kèm với sự thay đổi khả năng phản ứng với CGRP. Mật độ dây thần kinh giao cảm và PGP 9,5 không bị thay đổi bởi thai kỳ hoặc giới tính, và không có sự khác biệt về khả năng phản ứng với norepinephrine. Kết hợp lại, những kết quả này cho thấy rằng sự gia tăng sự chi phối của dây sinh ba trong thai kỳ có liên quan nhiều hơn đến cảm giác đau hơn là kiểm soát lưu lượng máu não khi nghỉ ngơi."} +{"idx": 1970122, "en": "Matrix metalloproteinases (MMPs) are now known to process a broad spectrum of cell surface molecules and to function in several important biological processes. Testing for differences in gene expression in human placental chorionic villi in the absence or presence of labor, using cDNA microarray analysis, revealed that labor was associated with increased expression of MMP-1 gene expression in 5 placentas collected after term normal spontaneous deliveries compared with 5 placentas collected after term nonlaboring cesarean deliveries. Fibronectin 1 and collagen XVII, 2 other proteins involved in the homeostasis of the extracellular matrix, were also found to be upregulated in labor. MMP-1 was further tested in individual samples and found to be consistently overexpressed in labor. While previous microarray analyses have focused on either uterine tissue or the fetal membranes, the data presented here indicate for the first time that placental chorionic villus genes are likely to affect the initiation of parturition through altered processing of cell surface molecules by MMP-1.", "gemini": "Hiện nay, người ta đã biết rằng các metalloproteinase ma trận (MMP) xử lý một loạt các phân tử bề mặt tế bào và hoạt động trong một số quá trình sinh học quan trọng. Kiểm tra sự khác biệt về biểu hiện gen ở nhung mao màng đệm nhau thai ở người khi không có hoặc có chuyển dạ, sử dụng phân tích mảng cDNA, cho thấy chuyển dạ có liên quan đến sự gia tăng biểu hiện gen MMP-1 ở 5 nhau thai thu thập được sau khi sinh tự nhiên đủ tháng so với 5 nhau thai thu thập được sau khi sinh mổ không chuyển dạ đủ tháng. Fibronectin 1 và collagen XVII, 2 protein khác liên quan đến cân bằng nội môi của ma trận ngoại bào, cũng được phát hiện là tăng lên trong quá trình chuyển dạ. MMP-1 đã được thử nghiệm thêm ở các mẫu riêng lẻ và thấy rằng nó luôn được biểu hiện quá mức trong quá trình chuyển dạ. Trong khi các phân tích mảng trước đây tập trung vào mô tử cung hoặc màng thai nhi, thì dữ liệu được trình bày ở đây chỉ ra lần đầu tiên rằng các gen nhung mao màng đệm nhau thai có khả năng ảnh hưởng đến quá trình chuyển dạ thông qua quá trình xử lý thay đổi các phân tử bề mặt tế bào bởi MMP-1."} +{"idx": 1970123, "en": "Low-frequency activity within the oculomotor system helps bridge sensation and action. Given ocular stability, low-frequency activity sustained by some neurons within the intermediate and deep superior colliculus (dSC) is assumed to be separated from motor output. However, the dSC is an orienting structure and the influence of low-frequency dSC activity at other effectors remains untested. We studied this by simultaneously recording activity from saccade-related dSC neurons and electromyographic (EMG) activity from neck muscles that turn the head. Monkeys performed a gap-saccade paradigm with varying levels of reward expectancy. Despite head restraint and even for relatively small target eccentricities (50 Hz, whereas in TA, one of four odorants produced a modest increment <40 Hz. LFP oscillations were clearly modulated by odorants during the TA task, increasing their oscillatory power at frequencies centered at 20 Hz and decreasing power at frequencies >50 Hz. Our results indicate that firing rate responses of MT cells in awake animals are behaviorally modulated with inhibition being a prominent feature of this modulation. The occurrence of oscillatory patterns in single- and multiunitary discharge is also related to stimulation and behavioral context, while the oscillatory patterns of the neuronal population showed a strong dependence on odorant stimulation.", "gemini": "Các chất tạo mùi gây ra sự điều chế cụ thể tốc độ bắn của các tế bào có tua/có chùm (MT) trong bóng khứu giác (OB) của động vật có vú, gây ra các kiểu thời gian phóng điện của nơ-ron được nhúng trong điện thế trường địa phương dao động (LFP). Trong khi hầu hết các nghiên cứu đã kiểm tra trên động vật được gây mê, thì người ta vẫn biết rất ít về tốc độ bắn và các kiểu thời gian của các đơn vị OB và hoạt động của quần thể ở động vật có vú khi tỉnh táo. Chúng tôi đã kiểm tra tốc độ bắn và hoạt động dao động của các tế bào MT và tín hiệu LFP ở chuột khi thực hiện hai nhiệm vụ khứu giác: tiếp xúc thụ động (PE) và phân biệt lựa chọn hai lựa chọn thay thế (TA). Các phản ứng ức chế MT chiếm ưu thế trong nhiệm vụ TA (76,5%), trong khi các phản ứng kích thích MT chiếm ưu thế trong nhiệm vụ PE (59,2%). Phóng điện có nhịp điệu trong phạm vi 12 đến 100 Hz được tìm thấy ở 79,0 và 68,9% các tế bào MT trong các nhiệm vụ PE và TA. Hầu hết các chất tạo mùi có trong nhiệm vụ PE làm tăng các xung nhịp ở tần số >50 Hz, trong khi trong TA, một trong bốn chất tạo mùi tạo ra mức tăng khiêm tốn <40 Hz. Các dao động LFP được điều chế rõ ràng bởi các chất tạo mùi trong nhiệm vụ TA, làm tăng công suất dao động của chúng ở các tần số tập trung ở 20 Hz và giảm công suất ở các tần số >50 Hz. Kết quả của chúng tôi chỉ ra rằng các phản ứng tốc độ bắn của các tế bào MT ở động vật khi tỉnh táo được điều chế theo hành vi, với sự ức chế là một đặc điểm nổi bật của sự điều chế này. Sự xuất hiện của các kiểu dao động trong xung nhịp đơn lẻ và đa đơn vị cũng liên quan đến kích thích và bối cảnh hành vi, trong khi các kiểu dao động của quần thể nơ-ron cho thấy sự phụ thuộc mạnh vào kích thích khứu giác."} +{"idx": 1970132, "en": "Previous neurophysiological studies of the frontal eye field (FEF) in monkeys have focused on its role in saccade target selection and gaze shift control. It has been argued that FEF neurons indicate the locations of behaviorally significant visual stimuli and are not inherently sensitive to specific features of the visual stimuli per se. Here, for the first time, we directly examined single cell responses to simple, two-dimensional shapes and found that shape selectivity exists in a substantial number of FEF cells during a passive fixation task or during the sample, delay (memory), and eye movement periods in a delayed match to sample (DMTS) task. Our data demonstrate that FEF neurons show sensory and mnemonic selectivity for stimulus shape features whether or not they are behaviorally significant for the task at hand. We also investigated the extent and localization of activation in the FEF using a variety of shape stimuli defined by static or dynamic cues employing functional magentic resonance imaging (fMRI) in anesthetized and paralyzed monkeys. Our fMRI results support the electrophysiological findings by showing significant FEF activation for a variety of shape stimuli and cues in the absence of attentional and motor processing. This shape selectivity in FEF is comparable to previous reports in the ventral pathway, inviting a reconsideration of the functional organization of the visual system.", "gemini": "Các nghiên cứu trước đây về sinh lý thần kinh của vùng nhãn cầu trán (FEF) ở loài khỉ tập trung vào vai trò của vùng này trong việc lựa chọn mục tiêu chuyển động mắt và kiểm soát chuyển động nhìn. Người ta cho rằng các tế bào thần kinh FEF chỉ ra vị trí của các kích thích thị giác có ý nghĩa về mặt hành vi và bản thân chúng không nhạy cảm với các đặc điểm cụ thể của các kích thích thị giác. Ở đây, lần đầu tiên, chúng tôi trực tiếp kiểm tra phản ứng của tế bào đơn lẻ đối với các hình dạng hai chiều đơn giản và phát hiện ra rằng tính chọn lọc hình dạng tồn tại trong một số lượng đáng kể các tế bào FEF trong nhiệm vụ cố định thụ động hoặc trong các giai đoạn mẫu, trì hoãn (trí nhớ) và chuyển động mắt trong một nhiệm vụ khớp trễ với mẫu (DMTS). Dữ liệu của chúng tôi chứng minh rằng các tế bào thần kinh FEF thể hiện tính chọn lọc về cảm giác và trí nhớ đối với các đặc điểm hình dạng của kích thích cho dù chúng có ý nghĩa về mặt hành vi đối với nhiệm vụ trong tầm tay hay không. Chúng tôi cũng đã điều tra mức độ và vị trí kích hoạt trong FEF bằng cách sử dụng nhiều loại kích thích hình dạng được xác định bởi các tín hiệu tĩnh hoặc động sử dụng hình ảnh cộng hưởng từ chức năng (fMRI) ở những con khỉ bị gây mê và liệt. Kết quả fMRI của chúng tôi hỗ trợ các phát hiện về điện sinh lý bằng cách chỉ ra sự kích hoạt FEF đáng kể đối với nhiều loại kích thích hình dạng và tín hiệu khi không có sự chú ý và xử lý vận động. Tính chọn lọc hình dạng này trong FEF tương đương với các báo cáo trước đây trong đường dẫn bụng, đưa ra lời mời xem xét lại tổ chức chức năng của hệ thống thị giác."} +{"idx": 1970133, "en": "It is well established that parkinsonian syndrome is associated with alterations in the temporal pattern of neuronal activity and local field potentials in the basal ganglia (BG). An increase in synchronized oscillations has been observed in different BG nuclei in parkinsonian patients and animal models of this disease. However, the mechanisms underlying this phenomenon remain unclear. This study investigates the functional connectivity in the cortex-BG network of a rodent model of Parkinson's disease. Single neurons and local field potentials were simultaneously recorded in the motor cortex, the striatum, and the substantia nigra pars reticulata (SNr) of freely moving rats, and high-voltage spindles (HVSs) were used to compare signal transmission before and after dopaminergic depletion. It is shown that dopaminergic lesion results in a significant enhancement of oscillatory synchronization in the BG: the coherence between pairs of structures increased significantly and the percentage of oscillatory auto- and cross-correlograms. HVS episodes were also more numerous and longer. These changes were associated with a shortening of the latency of SNr response to cortical activation, from 40.5 +/- 4.8 to 10.2 +/- 1.07 ms. This result suggests that, in normal conditions, SNr neurons are likely to be driven by late inputs from the indirect pathway; however, after the lesion, their shorter latency also indicates an overactivation of the hyperdirect pathway. This study confirms that neuronal signal transmission is altered in the BG after dopamine depletion but also provides qualitative evidence for these changes at the cellular level.", "gemini": "Người ta đã xác định rõ rằng hội chứng parkinson liên quan đến những thay đổi trong kiểu thời gian của hoạt động nơ-ron và điện thế trường cục bộ ở hạch nền (BG). Sự gia tăng dao động đồng bộ đã được quan sát thấy ở các nhân BG khác nhau ở bệnh nhân parkinson và các mô hình động vật mắc bệnh này. Tuy nhiên, cơ chế cơ bản của hiện tượng này vẫn chưa rõ ràng. Nghiên cứu này điều tra kết nối chức năng trong mạng lưới vỏ não-BG của một mô hình động vật mắc bệnh Parkinson. Các tế bào thần kinh đơn lẻ và điện thế trường cục bộ được ghi đồng thời ở vỏ não vận động, thể vân và phần lưới chất đen (SNr) của những con chuột di chuyển tự do và các trục chính điện áp cao (HVS) được sử dụng để so sánh truyền tín hiệu trước và sau khi cạn kiệt dopamin. Người ta thấy rằng tổn thương dopamin dẫn đến sự tăng cường đáng kể sự đồng bộ dao động trong BG: sự kết hợp giữa các cặp cấu trúc tăng lên đáng kể và tỷ lệ phần trăm tự động dao động và liên kết chéo. Các tập HVS cũng nhiều hơn và dài hơn. Những thay đổi này liên quan đến việc rút ngắn thời gian phản ứng SNr với sự kích hoạt vỏ não, từ 40,5 +/- 4,8 đến 10,2 +/- 1,07 ms. Kết quả này cho thấy, trong điều kiện bình thường, các tế bào thần kinh SNr có khả năng bị điều khiển bởi các đầu vào trễ từ đường gián tiếp; tuy nhiên, sau khi tổn thương, thời gian tiềm ẩn ngắn hơn của chúng cũng cho thấy sự hoạt động quá mức của đường siêu trực tiếp. Nghiên cứu này xác nhận rằng truyền tín hiệu nơ-ron bị thay đổi trong BG sau khi cạn kiệt dopamine nhưng cũng cung cấp bằng chứng định tính cho những thay đổi này ở cấp độ tế bào."} +{"idx": 1970134, "en": "The neonatal period is critical for seizure susceptibility, and neocortical networks are central in infantile epilepsies. We report that application of 4-aminopyridine (4-AP) to immature (P6-P9) neocortical slices generates layer-specific interictal seizures (IISs) that transform after recurrent seizures to ictal seizures (ISs). During IISs, cell-attached recordings show action potentials in interneurons and pyramidal cells in L5/6 and interneurons but not pyramidal neurons in L2/3. However, L2/3 pyramidal neurons also fire during ISs. Using single N-methyl-d-aspartate (NMDA) channel recordings for measuring the cell resting potential (Em), we show that transition from IISs to ISs is associated with a gradual Em depolarization of L2/3 and L5/6 pyramidal neurons that enhances their excitability. Bumetanide, a NKCC1 co-transporter antagonist, inhibits generation of IISs and prevents their transformation to ISs, indicating the role excitatory GABA in epilepsies. Therefore deep layer neurons are more susceptible to seizures than superficial ones. The initiating phase of seizures is characterized by IISs generated in L5/6 and supported by activation of both L5/6 interneurons and pyramidal cells. IISs propagate to L2/3 via activation of L2/3 interneurons but not pyramidal cells, which are mostly quiescent at this phase. In superficial layers, a persistent increase in excitability of pyramidal neurons caused by Em depolarization is associated with a transition from largely confined GABAergic IIS to ictal events that entrain the entire neocortex.", "gemini": "Giai đoạn sơ sinh rất quan trọng đối với tình trạng dễ bị động kinh, và các mạng lưới vỏ não mới là trung tâm trong bệnh động kinh ở trẻ sơ sinh. Chúng tôi báo cáo rằng việc sử dụng 4-aminopyridine (4-AP) cho các lát cắt vỏ não mới chưa trưởng thành (P6-P9) tạo ra các cơn động kinh liên vùng (IIS) đặc hiệu theo lớp, chuyển thành các cơn động kinh (IS) sau các cơn động kinh tái phát. Trong quá trình IIS, các bản ghi gắn tế bào cho thấy tiềm hành động ở các tế bào thần kinh trung gian và các tế bào hình chóp ở L5/6 và các tế bào thần kinh trung gian nhưng không phải các tế bào hình chóp ở L2/3. Tuy nhiên, các tế bào hình chóp L2/3 cũng hoạt động trong quá trình IS. Sử dụng các bản ghi kênh N-methyl-d-aspartate (NMDA) đơn lẻ để đo điện thế nghỉ của tế bào (Em), chúng tôi thấy rằng quá trình chuyển đổi từ IIS sang IS liên quan đến sự khử cực Em dần dần của các tế bào hình chóp L2/3 và L5/6, làm tăng khả năng kích thích của chúng. Bumetanide, một chất đối kháng đồng vận chuyển NKCC1, ức chế sự tạo ra IIS và ngăn chặn sự chuyển đổi của chúng thành IS, chỉ ra vai trò của GABA kích thích trong bệnh động kinh. Do đó, các tế bào lớp sâu dễ bị động kinh hơn các tế bào nông. Giai đoạn khởi phát của động kinh được đặc trưng bởi IIS được tạo ra ở L5/6 và được hỗ trợ bởi sự kích hoạt của cả tế bào thần kinh trung gian L5/6 và các tế bào hình chóp. IIS lan truyền đến L2/3 thông qua sự kích hoạt của các tế bào thần kinh trung gian L2/3 nhưng không phải các tế bào hình chóp, hầu hết đều ở trạng thái tĩnh lặng ở giai đoạn này. Ở các lớp nông, sự gia tăng liên tục về khả năng kích thích của các tế bào hình chóp do sự khử cực Em liên quan đến sự chuyển đổi từ IIS GABAergic bị giới hạn phần lớn sang các sự kiện động kinh kéo theo toàn bộ vỏ não mới."} +{"idx": 1970135, "en": "GABA depolarizes immature cortical neurons. However, whether GABA excites immature neocortical neurons and drives network oscillations as in other brain structures remains controversial. Excitatory actions of GABA depend on three fundamental parameters: the resting membrane potential (Em), reversal potential of GABA (E(GABA)), and threshold of action potential generation (Vthr). We have shown recently that conventional invasive recording techniques provide an erroneous estimation of these parameters in immature neurons. In this study, we used noninvasive single N-methyl-d-aspartate and GABA channel recordings in rodent brain slices to measure both Em and E(GABA) in the same neuron. We show that GABA strongly depolarizes pyramidal neurons and interneurons in both deep and superficial layers of the immature neocortex (P2-P10). However, GABA generates action potentials in layer 5/6 (L5/6) but not L2/3 pyramidal cells, since L5/6 pyramidal cells have more depolarized resting potentials and more hyperpolarized Vthr. The excitatory GABA transiently drives oscillations generated by L5/6 pyramidal cells and interneurons during development (P5-P12). The NKCC1 co-transporter antagonist bumetanide strongly reduces [Cl(-)]i, GABA-induced depolarization, and network oscillations, confirming the importance of GABA signaling. Thus a strong GABA excitatory drive coupled with high intrinsic excitability of L5/6 pyramidal neurons and interneurons provide a powerful mechanism of synapse-driven oscillatory activity in the rodent neocortex in vitro. In the companion paper, we show that the excitatory GABA drives layer-specific seizures in the immature neocortex.", "gemini": "GABA khử cực các nơ-ron vỏ não chưa trưởng thành. Tuy nhiên, liệu GABA có kích thích các nơ-ron tân vỏ não chưa trưởng thành và thúc đẩy dao động mạng như ở các cấu trúc não khác vẫn còn gây tranh cãi. Các hoạt động kích thích của GABA phụ thuộc vào ba thông số cơ bản: điện thế màng nghỉ (Em), điện thế đảo ngược của GABA (E(GABA)) và ngưỡng tạo thế hoạt động (Vthr). Gần đây, chúng tôi đã chỉ ra rằng các kỹ thuật ghi xâm lấn thông thường cung cấp ước tính sai về các thông số này ở các nơ-ron chưa trưởng thành. Trong nghiên cứu này, chúng tôi đã sử dụng các bản ghi kênh GABA và N-methyl-d-aspartate đơn không xâm lấn ở các lát não gặm nhấm để đo cả Em và E(GABA) trong cùng một nơ-ron. Chúng tôi chỉ ra rằng GABA khử cực mạnh các nơ-ron hình chóp và các tế bào thần kinh đệm trong cả các lớp sâu và lớp nông của tân vỏ não chưa trưởng thành (P2-P10). Tuy nhiên, GABA tạo ra các thế hoạt động ở các tế bào hình chóp lớp 5/6 (L5/6) nhưng không phải ở các tế bào hình chóp L2/3, vì các tế bào hình chóp L5/6 có điện thế nghỉ khử cực hơn và Vthr siêu phân cực hơn. GABA kích thích thúc đẩy tạm thời các dao động do các tế bào hình chóp L5/6 và các tế bào thần kinh đệm tạo ra trong quá trình phát triển (P5-P12). Thuốc đối kháng đồng vận chuyển NKCC1 bumetanide làm giảm mạnh [Cl(-)]i, khử cực do GABA gây ra và dao động mạng, xác nhận tầm quan trọng của tín hiệu GABA. Do đó, một động lực kích thích GABA mạnh kết hợp với khả năng kích thích nội tại cao của các tế bào hình chóp L5/6 và các tế bào thần kinh đệm cung cấp một cơ chế mạnh mẽ của hoạt động dao động do khớp thần kinh thúc đẩy trong tân vỏ não gặm nhấm trong ống nghiệm. Trong bài báo đi kèm, chúng tôi chỉ ra rằng GABA kích thích thúc đẩy các cơn động kinh đặc hiệu theo lớp ở tân vỏ não chưa trưởng thành."} +{"idx": 1970136, "en": "In vertebrate auditory systems, specialized combination-sensitive neurons analyze complex vocal signals by integrating information across multiple frequency bands. We studied combination-sensitive interactions in neurons of the inferior colliculus (IC) of awake mustached bats, using intracellular somatic recording with sharp electrodes. Facilitated combinatorial neurons are coincidence detectors, showing maximum facilitation when excitation from low- and high-frequency stimuli coincide. Previous work showed that facilitatory interactions originate in the IC, require both low and high frequency-tuned glycinergic inputs, and are independent of glutamatergic inputs. These results suggest that glycinergic inputs evoke facilitation through either postinhibitory rebound or direct depolarizing mechanisms. However, in 35 of 36 facilitated neurons, we observed no evidence of low frequency-evoked transient hyperpolarization or depolarization that was closely related to response facilitation. Furthermore, we observed no evidence of shunting inhibition that might conceal inhibitory inputs. Since these facilitatory interactions originate in IC neurons, the results suggest that inputs underlying facilitation are electrically segregated from the soma. We also recorded inhibitory combinatorial interactions, in which low frequency sounds suppress responses to higher frequency signals. In 43% of 118 neurons, we observed low frequency-evoked hyperpolarizations associated with combinatorial inhibition. For these neurons, we conclude that low frequency-tuned inhibitory inputs terminate on neurons primarily excited by high-frequency signals; these inhibitory inputs may create or enhance inhibitory combinatorial interactions. In the remainder of inhibited combinatorial neurons (57%), we observed no evidence of low frequency-evoked hyperpolarizations, consistent with observations that inhibitory combinatorial responses may originate in lateral lemniscal nuclei.", "gemini": "Trong các hệ thống thính giác của động vật có xương sống, các nơ-ron nhạy cảm với sự kết hợp chuyên biệt phân tích các tín hiệu giọng nói phức tạp bằng cách tích hợp thông tin trên nhiều băng tần. Chúng tôi đã nghiên cứu các tương tác nhạy cảm với sự kết hợp ở các nơ-ron của đồi dưới (IC) của những con dơi có ria mép khi tỉnh táo, bằng cách sử dụng ghi nội bào soma với các điện cực sắc. Các nơ-ron kết hợp được tạo điều kiện thuận lợi là các máy dò trùng hợp, cho thấy sự tạo điều kiện thuận lợi tối đa khi sự kích thích từ các kích thích tần số thấp và tần số cao trùng hợp. Các công trình trước đây cho thấy các tương tác tạo điều kiện thuận lợi có nguồn gốc từ IC, đòi hỏi cả đầu vào glycinergic được điều chỉnh ở tần số thấp và tần số cao và không phụ thuộc vào đầu vào glutamatergic. Những kết quả này gợi ý rằng các đầu vào glycinergic gợi ra sự tạo điều kiện thuận lợi thông qua cơ chế phục hồi sau ức chế hoặc khử cực trực tiếp. Tuy nhiên, ở 35 trong số 36 nơ-ron được tạo điều kiện thuận lợi, chúng tôi không quan sát thấy bằng chứng nào về sự siêu phân cực hoặc khử cực thoáng qua do tần số thấp gây ra có liên quan chặt chẽ đến sự tạo điều kiện thuận lợi cho phản ứng. Hơn nữa, chúng tôi không quan sát thấy bằng chứng nào về sự ức chế phân luồng có thể che giấu các đầu vào ức chế. Vì các tương tác tạo điều kiện thuận lợi này có nguồn gốc từ các nơ-ron IC, nên các kết quả cho thấy rằng các đầu vào tạo điều kiện thuận lợi được phân tách về mặt điện khỏi soma. Chúng tôi cũng ghi lại các tương tác kết hợp ức chế, trong đó âm thanh tần số thấp ức chế phản ứng với các tín hiệu tần số cao hơn. Ở 43% trong số 118 nơ-ron, chúng tôi quan sát thấy sự siêu phân cực do tần số thấp gây ra liên quan đến sự ức chế kết hợp. Đối với những nơ-ron này, chúng tôi kết luận rằng các đầu vào ức chế được điều chỉnh ở tần số thấp kết thúc ở các nơ-ron chủ yếu được kích thích bởi các tín hiệu tần số cao; các đầu vào ức chế này có thể tạo ra hoặc tăng cường các tương tác kết hợp ức chế. Ở phần còn lại của các nơ-ron kết hợp bị ức chế (57%), chúng tôi không quan sát thấy bằng chứng về sự siêu phân cực do tần số thấp gây ra, phù hợp với các quan sát cho thấy các phản ứng kết hợp ức chế có thể có nguồn gốc từ các nhân lemniscal bên."} +{"idx": 1970137, "en": "This study was designed to differentiate between two models of motor lateralization: \"feedback corrections\" and dynamic dominance. Whereas the feedback correction hypothesis suggests that handedness reflects a dominant hemisphere advantage for visual-mediated correction processes, dynamic dominance proposes that each hemisphere has become specialized for distinct aspects of control. This model suggests that the dominant hemisphere is specialized for controlling task dynamics, as required for coordinating efficient trajectories, and the nondominant hemisphere is specialized for controlling limb impedance, as required for maintaining stable postures. To differentiate between these two models, we examined whether visuomotor corrections are mediated differently for the nondominant and dominant arms. Participants performed targeted reaches in a virtual reality environment in which visuomotor rotations occurred in two directions that elicited corrections with different coordination requirements. The feedback correction model predicts a dominant arm advantage for the timing and accuracy of corrections in both directions. Dynamic dominance predicts that correction timing and accuracy will be similar for both arms, but that interlimb differences in the quality of corrections will depend on the coordination requirements, and thus, direction of corrections. Our results indicated that correction time and accuracy did not depend on arm. However, correction quality, as reflected by trajectory curvature, depended on both arm and rotation direction. Nondominant trajectories were systematically more curvilinear than dominant trajectories for corrections with the highest coordination requirement. These results support the dynamic dominance hypothesis.", "gemini": "Nghiên cứu này được thiết kế để phân biệt giữa hai mô hình về sự thuận tay vận động: \"sửa lỗi phản hồi\" và sự thống trị động. Trong khi giả thuyết sửa lỗi phản hồi cho rằng sự thuận tay phản ánh lợi thế của bán cầu não trội đối với các quá trình hiệu chỉnh do thị giác trung gian, sự thống trị động đề xuất rằng mỗi bán cầu não đã trở nên chuyên biệt cho các khía cạnh kiểm soát riêng biệt. Mô hình này cho rằng bán cầu não trội chuyên về kiểm soát động lực học nhiệm vụ, như được yêu cầu để phối hợp các quỹ đạo hiệu quả, và bán cầu não không trội chuyên về kiểm soát trở kháng của chi, như được yêu cầu để duy trì tư thế ổn định. Để phân biệt giữa hai mô hình này, chúng tôi đã kiểm tra xem các hiệu chỉnh thị giác vận động có được trung gian khác nhau đối với cánh tay không trội và cánh tay trội hay không. Những người tham gia đã thực hiện các lần với mục tiêu trong môi trường thực tế ảo, trong đó các vòng quay thị giác vận động xảy ra theo hai hướng gây ra các hiệu chỉnh với các yêu cầu phối hợp khác nhau. Mô hình hiệu chỉnh phản hồi dự đoán lợi thế của cánh tay trội về thời gian và độ chính xác của các hiệu chỉnh theo cả hai hướng. Sự thống trị động dự đoán rằng thời gian và độ chính xác của hiệu chỉnh sẽ tương tự nhau đối với cả hai cánh tay, nhưng sự khác biệt giữa các chi về chất lượng của các hiệu chỉnh sẽ phụ thuộc vào các yêu cầu phối hợp và do đó, hướng của các hiệu chỉnh. Kết quả của chúng tôi chỉ ra rằng thời gian và độ chính xác của hiệu chỉnh không phụ thuộc vào cánh tay. Tuy nhiên, chất lượng hiệu chỉnh, được phản ánh bằng độ cong quỹ đạo, phụ thuộc vào cả cánh tay và hướng quay. Các quỹ đạo không trội có hệ thống cong hơn các quỹ đạo trội đối với các hiệu chỉnh có yêu cầu phối hợp cao nhất. Những kết quả này hỗ trợ cho giả thuyết về sự thống trị động."} +{"idx": 1970138, "en": "Behavioral experiments indicate that central pathways that process otolith-ocular and perceptual information have adaptive capabilities. Because polarization vectors of otolith afferents are directly related to the electro-mechanical properties of the hair cell bundle, it is unlikely that they change their direction of excitation. This indicates that the adaptation must take place in central pathways. Here we demonstrate for the first time that otolith polarization vectors of canal-otolith convergent neurons in the vestibular nuclei have adaptive capability. A total of 10 vestibular-only and vestibular-plus-saccade neurons were recorded extracellularly in two monkeys before and after they were in side-down positions for 2 h. The spatial characteristics of the otolith input were determined from the response vector orientation (RVO), which is the projection of the otolith polarization vector, onto the head horizontal plane. The RVOs had no specific orientation before animals were in side-down positions but moved toward the gravitational axis after the animals were tilted for extended periods. Vector reorientations varied from 0 to 109 degrees and were linearly related to the original deviation of the RVOs from gravity in the position of adaptation. Such reorientation of central polarization vectors could provide the basis for changes in perception and eye movements related to prolonged head tilts relative to gravity or in microgravity.", "gemini": "Các thí nghiệm về hành vi cho thấy các đường dẫn trung tâm xử lý thông tin về mắt-tiền đình và nhận thức có khả năng thích ứng. Vì các vectơ phân cực của các sợi hướng tâm tiền đình liên quan trực tiếp đến các tính chất điện cơ của bó tế bào lông nên không có khả năng chúng thay đổi hướng kích thích của chúng. Điều này chỉ ra rằng sự thích ứng phải diễn ra ở các đường dẫn trung tâm. Ở đây, chúng tôi chứng minh lần đầu tiên rằng các vectơ phân cực tiền đình của các nơ-ron hội tụ tiền đình-ống bán khuyên ở nhân tiền đình có khả năng thích ứng. Tổng cộng 10 nơ-ron chỉ tiền đình và tiền đình cộng với chuyển động mắt nhanh đã được ghi lại ngoại bào ở hai con khỉ trước và sau khi chúng ở tư thế nằm ngửa trong 2 giờ. Các đặc điểm không gian của đầu vào tiền đình được xác định từ hướng vectơ đáp ứng (RVO), là phép chiếu của vectơ phân cực tiền đình, lên mặt phẳng ngang của đầu. Các RVO không có hướng cụ thể trước khi các động vật ở tư thế nằm ngửa nhưng di chuyển về phía trục trọng lực sau khi các động vật bị nghiêng trong thời gian dài. Các định hướng lại vectơ thay đổi từ 0 đến 109 độ và có liên quan tuyến tính với độ lệch ban đầu của các RVO so với trọng lực ở vị trí thích ứng. Sự định hướng lại của các vectơ phân cực trung tâm như vậy có thể cung cấp cơ sở cho những thay đổi về nhận thức và chuyển động mắt liên quan đến việc nghiêng đầu kéo dài so với trọng lực hoặc trong điều kiện vi trọng lực."} +{"idx": 1970139, "en": "A number of studies have been interpreted to support the view that the inferior olive climbing fibers send periodic signals to the cerebellum to time and pace behavior. In a direct test of this hypothesis in macaques performing nonperiodic tasks, we analyzed continuous recordings of complex spikes from the lateral cerebellar hemisphere. We found no periodicity outside of a 100-ms relative refractory period.", "gemini": "Một số nghiên cứu đã được giải thích để hỗ trợ quan điểm cho rằng các sợi leo ô liu dưới gửi tín hiệu định kỳ đến tiểu não để định thời gian và nhịp độ cho hành vi. Trong một thử nghiệm trực tiếp về giả thuyết này ở loài khỉ thực hiện các nhiệm vụ không định kỳ, chúng tôi đã phân tích các bản ghi liên tục các xung phức từ bán cầu tiểu não bên. Chúng tôi không tìm thấy tính chu kỳ nào nằm ngoài chu kỳ chịu lửa tương đối 100 ms."} +{"idx": 1970140, "en": "Antisaccades are known to show greater variable error and also a systematic hypometria in their amplitude compared with visually guided prosaccades. In this study, we examined whether their accuracy in direction (as opposed to amplitude) also showed a systematic error. We had human subjects perform prosaccades and antisaccades to goals located at a variety of polar angles. In the first experiment, subjects made prosaccades or antisaccades to one of eight equidistant locations in each block, whereas in the second, they made saccades to one of two equidistant locations per block. In the third, they made antisaccades to one of two locations at different distances but with the same polar angle in each block. Regardless of block design, the results consistently showed a saccadic systematic error, in that oblique antisaccades (but not prosaccades) requiring unequal vertical and horizontal vector components were deviated toward the 45 degrees diagonal meridians. This finding could not be attributed to range effects in either Cartesian or polar coordinates. A perceptual origin of the diagonal effect is suggested by similar systematic errors in other studies of memory-guided manual reaching or perceptual estimation of direction, and may indicate a common spatial bias when there is uncertain information about spatial location.", "gemini": "Antisaccades được biết là biểu hiện lỗi thay đổi lớn hơn và cũng là một hiện tượng hạ huyết áp hệ thống về biên độ của chúng so với các prosaccades được hướng dẫn bằng thị giác. Trong nghiên cứu này, chúng tôi đã kiểm tra xem độ chính xác về hướng (trái ngược với biên độ) của chúng cũng có biểu hiện lỗi hệ thống hay không. Chúng tôi đã yêu cầu những người tham gia thực hiện các prosaccades và antisaccades đến các mục tiêu nằm ở nhiều góc cực khác nhau. Trong thí nghiệm đầu tiên, đối tượng thực hiện prosaccades hoặc antisaccades đến một trong tám vị trí cách đều nhau trong mỗi khối, trong khi ở thí nghiệm thứ hai, họ thực hiện các chuyển động mắt nhanh đến một trong hai vị trí cách đều nhau trên mỗi khối. Trong thí nghiệm thứ ba, họ thực hiện các antisaccades đến một trong hai vị trí ở các khoảng cách khác nhau nhưng có cùng góc cực trong mỗi khối. Bất kể thiết kế khối nào, kết quả đều nhất quán cho thấy một lỗi hệ thống theo chuyển động mắt nhanh, ở chỗ các antisaccades chéo (nhưng không phải prosaccades) yêu cầu các thành phần vectơ dọc và ngang không bằng nhau bị lệch về phía các kinh tuyến chéo 45 độ. Phát hiện này không thể được quy cho các hiệu ứng phạm vi trong hệ tọa độ Descartes hoặc hệ tọa độ cực. Nguồn gốc nhận thức của hiệu ứng đường chéo được gợi ý bởi các lỗi hệ thống tương tự trong các nghiên cứu khác về việc với tay theo hướng dẫn của trí nhớ hoặc ước tính hướng nhận thức, và có thể chỉ ra một sai lệch không gian chung khi có thông tin không chắc chắn về vị trí không gian."} +{"idx": 1970141, "en": "Generalization from single-case designs can be achieved by replicating individual studies across different experimental units and settings. When replications are available, their findings can be summarized using effect size measurements and integrated through meta-analyses. Several procedures are available for quantifying the magnitude of treatment effect in N = 1 designs, and some of them are studied in this article. Monte Carlo simulations were used to generate different data patterns (trend, level change, and slope change). The experimental conditions simulated were defined by the degrees of serial dependence and phase length. Out of all the effect size indices studied, the percentage of nonoverlapping data and standardized mean difference proved to be less affected by autocorrelation and to perform better for shorter data series. The regression-based procedures proposed specifically for single-case designs did not differentiate between data patterns as well as did simpler indices.", "gemini": "Có thể đạt được sự khái quát từ các thiết kế trường hợp đơn lẻ bằng cách sao chép các nghiên cứu riêng lẻ trên các đơn vị và bối cảnh thử nghiệm khác nhau. Khi có bản sao, có thể tóm tắt các phát hiện của chúng bằng cách sử dụng phép đo kích thước hiệu ứng và tích hợp thông qua phân tích tổng hợp. Có một số thủ tục để định lượng độ lớn của hiệu ứng điều trị trong các thiết kế N = 1 và một số trong số chúng được nghiên cứu trong bài báo này. Các mô phỏng Monte Carlo đã được sử dụng để tạo ra các mẫu dữ liệu khác nhau (xu hướng, thay đổi mức độ và thay đổi độ dốc). Các điều kiện thử nghiệm được mô phỏng được xác định bởi các mức độ phụ thuộc tuần tự và chiều dài pha. Trong số tất cả các chỉ số kích thước hiệu ứng được nghiên cứu, tỷ lệ dữ liệu không chồng chéo và chênh lệch trung bình chuẩn hóa được chứng minh là ít bị ảnh hưởng bởi tự tương quan và hoạt động tốt hơn đối với các chuỗi dữ liệu ngắn hơn. Các thủ tục dựa trên hồi quy được đề xuất cụ thể cho các thiết kế trường hợp đơn lẻ không phân biệt giữa các mẫu dữ liệu cũng như các chỉ số đơn giản hơn."} +{"idx": 1970142, "en": "Apolipoprotein E (apoE) plays important roles in lipid homeostasis, anti-inflammation, and host defense. Since tissue apoE mRNA levels have been reported to decrease during inflammatory responses, we were surprised to find that plasma apoE levels were significantly elevated during septic infections in both humans and mice. This apparent paradox was also observed during lipopolysaccharide-induced acute inflammation in mice: plasma levels of apoE increased up to 4-fold despite sharply decreased apoE gene expression in the liver, macrophages, and extrahepatic tissues. We hypothesized that apoE levels were augmented by decreased plasma clearance. Our analysis revealed that apoE associated principally with HDL in mice and that apoE was cleared from the circulation principally via LDL receptors. The acute inflammatory response decreased LDL receptor expression in the liver and significantly reduced the rate of apoE clearance. In contrast, the same inflammatory stimuli increased LDL receptor expression in macrophages. Our results define a novel acute phase mechanism that increases circulating apoE levels as apoE production decreases. Diminished hepatic LDL receptor expression may thus cooperate with elevated LDL receptor expression in macrophages to facilitate the forward transport of apoE and its associated lipids to these key defense cells.", "gemini": "Apolipoprotein E (apoE) đóng vai trò quan trọng trong cân bằng lipid, chống viêm và bảo vệ vật chủ. Vì nồng độ mRNA apoE trong mô đã được báo cáo là giảm trong quá trình đáp ứng viêm, chúng tôi rất ngạc nhiên khi thấy rằng nồng độ apoE trong huyết tương tăng đáng kể trong quá trình nhiễm trùng nhiễm trùng ở cả người và chuột. Nghịch lý rõ ràng này cũng được quan sát thấy trong quá trình viêm cấp tính do lipopolysaccharide gây ra ở chuột: nồng độ apoE trong huyết tương tăng tới 4 lần mặc dù biểu hiện gen apoE giảm mạnh ở gan, đại thực bào và các mô ngoài gan. Chúng tôi đưa ra giả thuyết rằng nồng độ apoE tăng lên là do giảm độ thanh thải trong huyết tương. Phân tích của chúng tôi cho thấy apoE chủ yếu liên kết với HDL ở chuột và apoE được thanh thải khỏi tuần hoàn chủ yếu thông qua các thụ thể LDL. Phản ứng viêm cấp tính làm giảm biểu hiện thụ thể LDL ở gan và làm giảm đáng kể tốc độ thanh thải apoE. Ngược lại, cùng một kích thích viêm làm tăng biểu hiện thụ thể LDL ở đại thực bào. Kết quả của chúng tôi xác định một cơ chế pha cấp tính mới làm tăng nồng độ apoE lưu hành khi sản xuất apoE giảm. Do đó, biểu hiện thụ thể LDL ở gan giảm có thể hợp tác với biểu hiện thụ thể LDL tăng ở đại thực bào để tạo điều kiện thuận lợi cho quá trình vận chuyển apoE và các lipid liên quan đến các tế bào phòng thủ chính này."} +{"idx": 1970143, "en": "To study various polyhydroxy-alkaloid glycosidase inhibitors, 16 groups of 3 mice were dosed using osmotic minipumps with swainsonine (0, 0.1, 1, and 10 mg/kg/day), castanospermine, and calystegines A(3), B(2), and C(1) (1, 10, and 100 mg/kg/day). After 28 days, the mice were euthanized, necropsied, and examined using light and electron microscopy. The high-dose swainsonine-treated mice developed neurologic disease with neuro-visceral vacuolation typical of locoweed poisoning. Castanospermine- and calystegines-treated mice were clinically normal; however, high-dose castanospermine-treated mice had thyroid, renal, hepatic, and skeletal myocyte vacuolation. Histochemically, swainsonine- and castanospermine-induced vacuoles contained mannose-rich oligosaccharides. High-dose calystegine A(3)-treated mice had increased numbers of granulated cells in the hepatic sinusoids. Electron microscopy, lectin histochemistry, and immunohistochemistry suggest these are pit cells (specialized NK cells). Histochemically, the granules contain glycoproteins or oligosaccharides with abundant terminal N-acetylglucosamine residues. Other calystegine-treated mice were histologically normal. These findings indicate that swainsonine produced lesions similar to locoweed, castanospermine caused vacuolar changes with minor changes in glycogen metabolism, and only calystegine A(3) produced minimal hepatic changes. These also suggest that in mice calystegines and castanospermine are less toxic than swainsonine, and as rodents are relatively resistant to disease, they are poor models to study such induced storage diseases.", "gemini": "Để nghiên cứu các chất ức chế glycosidase polyhydroxy-alkaloid khác nhau, 16 nhóm gồm 3 con chuột đã được dùng liều bằng máy bơm thẩm thấu với swainsonine (0, 0,1, 1 và 10 mg / kg / ngày), castanospermine và calystegines A (3), B (2) và C (1) (1, 10 và 100 mg / kg / ngày). Sau 28 ngày, những con chuột được gây chết êm, khám nghiệm tử thi và kiểm tra bằng kính hiển vi quang học và điện tử. Những con chuột được điều trị bằng swainsonine liều cao phát triển bệnh thần kinh với hiện tượng rỗng nội tạng thần kinh điển hình của ngộ độc locoweed. Những con chuột được điều trị bằng castanospermine và calystegines về mặt lâm sàng là bình thường; tuy nhiên, những con chuột được điều trị bằng castanospermine liều cao có hiện tượng rỗng tuyến giáp, thận, gan và tế bào cơ xương. Về mặt mô học, các không bào do swainsonine và castanospermine gây ra chứa oligosacarit giàu mannose. Những con chuột được điều trị bằng calystegine A (3) liều cao có số lượng tế bào hạt tăng lên trong các xoang gan. Kính hiển vi điện tử, mô học lectin và miễn dịch mô học cho thấy đây là tế bào hố (tế bào NK chuyên biệt). Về mặt mô học, các hạt chứa glycoprotein hoặc oligosacarit với nhiều gốc N-acetylglucosamine đầu cuối. Những con chuột được điều trị bằng calystegine khác về mặt mô học là bình thường. Những phát hiện này chỉ ra rằng swainsonine gây ra các tổn thương tương tự như locoweed, castanospermine gây ra những thay đổi về không bào với những thay đổi nhỏ trong quá trình chuyển hóa glycogen và chỉ có calystegine A (3) gây ra những thay đổi tối thiểu ở gan. Những điều này cũng cho thấy rằng ở chuột, calystegines và castanospermine ít độc hơn swainsonine và vì loài gặm nhấm tương đối đề kháng với bệnh, chúng là những mô hình kém để nghiên cứu các bệnh lưu trữ do cảm ứng như vậy."} diff --git a/retranslated_vi_pubmed/chunk_197/error.json b/retranslated_vi_pubmed/chunk_197/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_197/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_198/completed.json b/retranslated_vi_pubmed/chunk_198/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..afdb55bb3e66e681025d0912b8cf9c4d15e96a3a --- /dev/null +++ b/retranslated_vi_pubmed/chunk_198/completed.json @@ -0,0 +1,146 @@ +[ + 1980000, + 1980001, + 1980002, + 1980003, + 1980004, + 1980005, + 1980006, + 1980007, + 1980008, + 1980009, + 1980010, + 1980011, + 1980012, + 1980013, + 1980014, + 1980015, + 1980016, + 1980017, + 1980018, + 1980019, + 1980020, + 1980021, + 1980022, + 1980023, + 1980024, + 1980025, + 1980026, + 1980027, + 1980028, + 1980029, + 1980030, + 1980031, + 1980032, + 1980033, + 1980034, + 1980035, + 1980036, + 1980037, + 1980038, + 1980039, + 1980040, + 1980041, + 1980042, + 1980043, + 1980044, + 1980045, + 1980046, + 1980047, + 1980048, + 1980049, + 1980050, + 1980051, + 1980052, + 1980053, + 1980054, + 1980055, + 1980056, + 1980057, + 1980058, + 1980059, + 1980060, + 1980061, + 1980062, + 1980063, + 1980064, + 1980065, + 1980066, + 1980067, + 1980068, + 1980069, + 1980070, + 1980071, + 1980072, + 1980073, + 1980074, + 1980075, + 1980076, + 1980077, + 1980078, + 1980079, + 1980080, + 1980081, + 1980082, + 1980083, + 1980084, + 1980085, + 1980086, + 1980087, + 1980088, + 1980089, + 1980090, + 1980091, + 1980092, + 1980093, + 1980094, + 1980095, + 1980096, + 1980097, + 1980098, + 1980099, + 1980100, + 1980101, + 1980102, + 1980103, + 1980104, + 1980105, + 1980106, + 1980107, + 1980108, + 1980109, + 1980110, + 1980111, + 1980112, + 1980113, + 1980114, + 1980115, + 1980116, + 1980117, + 1980118, + 1980119, + 1980120, + 1980121, + 1980122, + 1980123, + 1980124, + 1980125, + 1980126, + 1980127, + 1980128, + 1980129, + 1980130, + 1980131, + 1980132, + 1980133, + 1980134, + 1980135, + 1980136, + 1980137, + 1980138, + 1980139, + 1980140, + 1980141, + 1980142, + 1980143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_198/data.jsonl b/retranslated_vi_pubmed/chunk_198/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..693dcc9ebc7ffc2dae2f11299b929f57a59e7d6e --- /dev/null +++ b/retranslated_vi_pubmed/chunk_198/data.jsonl @@ -0,0 +1,144 @@ +{"idx": 1980000, "en": "Extramedullary plasmacytomas are plasma cell tumors that arise outside of the bone marrow. They account for approximately 3% of plasma cell neoplasms and are most frequently located in the head and neck region. Five months after undergoing cholecystectomy, a 69-year-old patient presented with the pain under the right costal margin and a 12 kg weight loss. Computed tomography of the abdomen demonstrated irregular, vascular mass in the gallbladder fossa that dents towards the duodenum and the pylorus and lowers caudally to the hepatic flexure. His laboratory tests indicated normocytic anemia and showed elevated sedimentation rate. During operative procedure, a tumorous mass in the gallbladder fossa was found, inseparable of the peritoneum of the hepatoduodenal ligament and the IVb liver segment. Histopathological examination and immunohistochemical staining determined the diagnosis of the plasmacytoma. Total resection of the tumor was achieved and after 24-month follow-up patient showed no signs of local recurrence or dissemination of the disease.", "gemini": "U tủy ngoài tủy là khối u tế bào plasma phát sinh ngoài tủy xương. Chúng chiếm khoảng 3% các khối u tân sinh tế bào plasma và thường gặp nhất ở vùng đầu và cổ. Năm tháng sau khi cắt túi mật, một bệnh nhân 69 tuổi đến khám vì đau dưới bờ sườn phải và sụt 12 kg. Chụp cắt lớp vi tính ổ bụng cho thấy khối u mạch máu không đều ở hố túi mật, ấn vào tá tràng và môn vị và hạ xuống góc gan. Xét nghiệm trong phòng thí nghiệm của ông cho thấy thiếu máu hồng cầu bình thường và tốc độ lắng hồng cầu tăng. Trong quá trình phẫu thuật, người ta phát hiện thấy một khối u ở hố túi mật, không thể tách rời khỏi phúc mạc của dây chằng gan tá tràng và phân gan IVb. Kiểm tra mô bệnh học và nhuộm miễn dịch mô hóa học xác định chẩn đoán u tủy. Toàn bộ khối u đã được cắt bỏ và sau 24 tháng theo dõi, bệnh nhân không có dấu hiệu tái phát tại chỗ hoặc di căn của bệnh."} +{"idx": 1980001, "en": "Ascending colon cancer as a colocutaneous fistula to the abdominal wall at the site of a previous postoperative scar is extremely rare. A 69 year old male presented with five day history of pain and foul smelling discharge from right subcostal scar from elective cholecystectomy performed 8 years ago. Last three days, he had fever up to 39 degrees C, with mild pain in right upper abdominal quadrant without vomiting, diarrhea, bloody stools or weight loss. Computed tomography, with peroral contrast, revealed extralumination into abdominal wall with several fistulas reaching the skin. Emergency median laparotomy found infiltrating tumor of ascending colon into abdominal wall. A right hemicolectomy and complete thickness abdominal wall excision (7 x 10 cm) was performed. The abdominal wall defect was too extensive for primary closure and two 20 x 20 cm moist gauzes were placed to cover the defect and were fixed with stitches to the skin. On second postoperative day, due to contamination, porcine dermal collagen implant was placed intraperitoneally. Such emergency presentations and therapeutic options are discussed.", "gemini": "Ung thư đại tràng lên tạo thành lỗ rò đại tràng da ở thành bụng tại vị trí sẹo sau mổ trước đó là cực kỳ hiếm gặp. Một nam giới 69 tuổi đến khám với tiền sử đau và dịch tiết có mùi hôi từ sẹo hạ sườn phải do cắt túi mật theo chỉ định cách đây 8 năm. Ba ngày gần đây, ông bị sốt lên đến 39 độ C, đau nhẹ ở góc bụng trên bên phải mà không kèm theo nôn, tiêu chảy, phân có máu hoặc sụt cân. Chụp cắt lớp vi tính có cản quang qua đường miệng cho thấy có hình ảnh thoát dịch ra ngoài thành bụng với nhiều lỗ rò thông ra da. Phẫu thuật nội soi ổ bụng cấp cứu thấy khối u xâm lấn đại tràng lên vào thành bụng. Đã tiến hành cắt đại tràng phải và cắt bỏ toàn bộ độ dày thành bụng (7 x 10 cm). Khiếm khuyết thành bụng quá rộng để đóng trực tiếp nên đã đặt hai miếng gạc ẩm 20 x 20 cm để che khiếm khuyết và cố định bằng chỉ khâu vào da. Vào ngày hậu phẫu thứ hai, do bị nhiễm trùng nên đã đặt miếng ghép collagen da lợn vào trong ổ bụng. Các biểu hiện cấp cứu và lựa chọn điều trị như vậy được thảo luận."} +{"idx": 1980002, "en": "Traumatic brain injuries represent a major cause of death and disability. We present a case of a 47-year-old patient who sustained a severe brain injury after being assaulted with a handle of an axe. The patient underwent numerous surgeries by various specialists during several months. Following a few failed attempts to cover the skull defects, the vacuum-assisted closure system had been utilized with great success in healing of her complex head wound. Traumatic brain injury requires great effort and collaboration in order to rehabilitate people to the most independent level of functioning possible.", "gemini": "Chấn thương sọ não là nguyên nhân chính gây tử vong và tàn tật. Chúng tôi trình bày trường hợp của một bệnh nhân 47 tuổi bị chấn thương não nghiêm trọng sau khi bị hành hung bằng cán rìu. Bệnh nhân đã trải qua nhiều cuộc phẫu thuật do nhiều chuyên gia khác nhau thực hiện trong nhiều tháng. Sau một vài lần cố gắng che phủ các khuyết hổng sọ không thành công, hệ thống đóng vết thương hỗ trợ chân không đã được sử dụng rất thành công trong việc chữa lành vết thương phức tạp ở đầu của cô ấy. Chấn thương sọ não đòi hỏi nhiều nỗ lực và sự hợp tác để phục hồi chức năng cho mọi người ở mức độ độc lập nhất có thể."} +{"idx": 1980003, "en": "The aim of the paper was to evaluate current emergency contraception (EC) methods and policies in order to implement lessons learned and maximize potential population impact while introducing dedicated EC pills in Croatia. Literature search for potential reasons for EC failing to show positive population impact and detecting actionable points to be implemented in national guidelines. Six potential reasons for ECs failure to show population impact were evaluated and four actionable points were detected: low use of EC compared to the numbers of risk events, low awareness on EC in general population, differences in efficacy of EC methods and EC vailability. In order to ensure EC's population impact in Croatia it is of a critical relevance to establish continuous education programs for population of women at risk. When recommending an EC method, superior efficacy must be a key decision-making criteria therefore cooper IUD and ulipristal acetate should be our primary options. Counseling is a critical step to ensure maximal efficacy of the EC method, but also to encourage future use of regular contraceptives. Finally, national ECP dispension protocol is needed to close the loop from effective women screening, prompt yet appropriate ECP administration/dispensing towards structured follow up after EC pills intake.", "gemini": "Mục đích của bài báo là đánh giá các phương pháp và chính sách tránh thai khẩn cấp (EC) hiện tại để thực hiện các bài học kinh nghiệm và tối đa hóa tác động tiềm tàng đối với dân số trong khi giới thiệu các loại thuốc tránh thai khẩn cấp chuyên dụng tại Croatia. Tìm kiếm tài liệu để tìm ra các lý do tiềm ẩn khiến EC không cho thấy tác động tích cực đến dân số và phát hiện các điểm có thể hành động cần được thực hiện trong các hướng dẫn quốc gia. Sáu lý do tiềm ẩn khiến EC không cho thấy tác động đến dân số đã được đánh giá và bốn điểm có thể hành động đã được phát hiện: tỷ lệ sử dụng EC thấp so với số lượng các sự kiện có nguy cơ, nhận thức về EC thấp trong dân số nói chung, sự khác biệt về hiệu quả của các phương pháp EC và khả năng cung cấp EC. Để đảm bảo tác động của EC đối với dân số ở Croatia, việc thiết lập các chương trình giáo dục liên tục cho nhóm phụ nữ có nguy cơ là rất quan trọng. Khi khuyến nghị một phương pháp EC, hiệu quả cao hơn phải là tiêu chí ra quyết định chính do đó vòng tránh thai bằng đồng và ulipristal acetate nên là lựa chọn chính của chúng tôi. Tư vấn là một bước quan trọng để đảm bảo hiệu quả tối đa của phương pháp EC, mà còn khuyến khích sử dụng các biện pháp tránh thai thường xuyên trong tương lai. Cuối cùng, cần có giao thức phân phối ECP quốc gia để khép lại vòng lặp từ việc sàng lọc phụ nữ hiệu quả, quản lý/phân phối ECP kịp thời nhưng phù hợp cho đến việc theo dõi có cấu trúc sau khi uống thuốc tránh thai khẩn cấp."} +{"idx": 1980004, "en": "The method for the \"Determination of Vitamins D2 and D3 in Infant Formula and Adult Nutritionals by Ultra-Pressure Liquid Chromatography with Tandem Mass Spectrometry Detection (UPLC-MS/MS)\" was adopted as AOAC Official First Action during the \"Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting\" held June 29, 2011. During the meeting, an Expert Review Panel (ERP) evaluated the available validation information against standard method performance requirements (SMPRs) articulated by stakeholders. The method, approved by the ERP, is applicable for the determination of vitamin D (total vitamins D2 and D3). A range of products had been tested during a single-laboratory validation study. The products included butter, National Institute of Standards and Technology SRM 1849, eggs, cheese, yogurt, ready-to-eat cereal, bread, mushrooms, and tuna. The testing of the method established linearity in the range of 0.005-50 microg/mL. The recovery range was 93.4-100.9% for vitamin D2 and 102.4-106.2% for vitamin D3. The LOD and LOQ for vitamin D2 were reported as 0.20 and 0.61 microgl100 g, respectively; for vitamin D3, the reported values were 0.47 and 1.44 microg/100 g, respectively. The method met the SMPRs set by the Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN). It was, therefore, decided that the method was appropriate for Official First Action Method status.", "gemini": "Phương pháp \"Xác định Vitamin D2 và D3 trong Sữa công thức dành cho trẻ sơ sinh và Dinh dưỡng người lớn bằng Sắc ký lỏng siêu áp suất với Phát hiện khối phổ song song (UPLC-MS/MS)\" đã được thông qua làm Hành động đầu tiên chính thức của AOAC trong \"Phát triển tiêu chuẩn và Hài hòa hóa quốc tế: Cuộc họp giữa năm của AOAC INTERNATIONAL\" được tổ chức vào ngày 29 tháng 6 năm 2011. Trong cuộc họp, một Hội đồng đánh giá chuyên gia (ERP) đã đánh giá thông tin xác thực có sẵn so với các yêu cầu về hiệu suất phương pháp tiêu chuẩn (SMPR) do các bên liên quan nêu ra. Phương pháp do ERP chấp thuận được áp dụng để xác định vitamin D (tổng lượng vitamin D2 và D3). Một loạt các sản phẩm đã được thử nghiệm trong một nghiên cứu xác thực của một phòng thí nghiệm duy nhất. Các sản phẩm bao gồm bơ, Viện Tiêu chuẩn và Công nghệ Quốc gia SRM 1849, trứng, pho mát, sữa chua, ngũ cốc ăn liền, bánh mì, nấm và cá ngừ. Việc thử nghiệm phương pháp đã thiết lập tính tuyến tính trong phạm vi 0,005-50 microg/mL. Phạm vi thu hồi là 93,4-100,9% đối với vitamin D2 và 102,4-106,2% đối với vitamin D3. LOD và LOQ đối với vitamin D2 lần lượt được báo cáo là 0,20 và 0,61 microgl100 g; đối với vitamin D3, các giá trị được báo cáo lần lượt là 0,47 và 1,44 microg/100 g. Phương pháp đáp ứng các SMPR do Hội đồng bên liên quan về Sữa công thức dành cho trẻ sơ sinh và Dinh dưỡng người lớn (SPIFAN) đặt ra. Do đó, người ta đã quyết định rằng phương pháp này phù hợp với trạng thái Phương pháp hành động đầu tiên chính thức."} +{"idx": 1980005, "en": "During the \"Standards Development and International Harmonization: AOAC INTERNATIONAL Mid-Year Meeting\" held on June 29, 2011, an Expert Review Panel (ERP) on behalf of AOAC INTERNATIONAL adopted the method \"Simultaneous Determination of Vitamins D2 and D3 by LC-MS/MS in Infant Formula and Adult Nutritionals\" as an AOAC Official First Action method. Vitamins D2 and D3 are extracted from the sample using pentane-ether; the extract is collected and dried under nitrogen. Vitamin D is separated from interfering compounds using UPLC, and quantitated using tandem mass spectrometry (MS/MS). Preliminary data showed the intermediate precision ranged from 3.34-8.05% and an accuracy range of 98.5-111% over the samples tested for vitamin D3. For vitamin D2, the intermediate precision ranged from 2.37-5.45% and accuracy ranged from 96.4-104% over the four matrixes evaluated. The analytical range for the method is bounded by the concentrations of the working standards, 21-270 ng/mL, and is equivalent to 0.168-2.16 mcg/100 g in ready-to-feed product. The practical method quantitation limit is 0.168 mcg/100 g product with method detection limit of 60 ng/100 g product. The ERP reviewed the data and determined that the performance characteristics of the method met the standard method performance requirements, and therefore the method was granted First Action status.", "gemini": "Trong \"Phát triển tiêu chuẩn và Hài hòa hóa quốc tế: Cuộc họp giữa năm của AOAC INTERNATIONAL\" được tổ chức vào ngày 29 tháng 6 năm 2011, một Hội đồng đánh giá chuyên gia (ERP) thay mặt cho AOAC INTERNATIONAL đã thông qua phương pháp \"Xác định đồng thời Vitamin D2 và D3 bằng LC-MS/MS trong Sữa công thức dành cho trẻ sơ sinh và Dinh dưỡng người lớn\" làm phương pháp Hành động đầu tiên chính thức của AOAC. Vitamin D2 và D3 được chiết xuất khỏi mẫu bằng pentane-ether; chiết xuất được thu thập và sấy khô dưới nitơ. Vitamin D được tách khỏi các hợp chất gây nhiễu bằng UPLC và định lượng bằng khối phổ song song (MS/MS). Dữ liệu sơ bộ cho thấy độ chính xác trung gian dao động từ 3,34-8,05% và độ chính xác trong khoảng 98,5-111% đối với các mẫu được thử nghiệm đối với vitamin D3. Đối với vitamin D2, độ chính xác trung gian dao động từ 2,37-5,45% và độ chính xác dao động từ 96,4-104% trên bốn ma trận được đánh giá. Phạm vi phân tích của phương pháp được giới hạn bởi nồng độ của các tiêu chuẩn làm việc, 21-270 ng/mL và tương đương với 0,168-2,16 mcg/100 g trong sản phẩm pha sẵn. Giới hạn định lượng phương pháp thực tế là 0,168 mcg/100 g sản phẩm với giới hạn phát hiện của phương pháp là 60 ng/100 g sản phẩm. ERP đã xem xét dữ liệu và xác định rằng các đặc điểm hiệu suất của phương pháp đáp ứng các yêu cầu về hiệu suất phương pháp tiêu chuẩn và do đó phương pháp này được cấp trạng thái Hành động đầu tiên."} +{"idx": 1980006, "en": "A method for the routine determination of 5'-mononucleotides (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate, and cytidine 5'-monophosphate) in infant formula and adult nutritionals is described. After sample dissolution and addition of internal standard, potential interferences were removed by anion-exchange SPE followed by HPLC-UV analysis. Single-laboratory validation performance parameters include recovery (92-101%) and repeatability (1.0-2.3% RSD). The method was approved for Official First Action status by an AOAC expert review panel.", "gemini": "Một phương pháp xác định thường quy 5'-mononucleotide (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate và cytidine 5'-monophosphate) trong sữa công thức dành cho trẻ sơ sinh và dinh dưỡng dành cho người lớn được mô tả. Sau khi hòa tan mẫu và thêm chuẩn nội, các tạp chất tiềm ẩn đã được loại bỏ bằng SPE trao đổi anion tiếp theo là phân tích HPLC-UV. Các thông số hiệu suất xác nhận của phòng thí nghiệm đơn bao gồm độ thu hồi (92-101%) và độ lặp lại (1,0-2,3% RSD). Phương pháp này đã được một nhóm chuyên gia đánh giá của AOAC chấp thuận cho trạng thái Hành động đầu tiên chính thức."} +{"idx": 1980007, "en": "Official Method 2011.21 is for the quantitation of the following nucleotides: adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), uridine 5'-monophosphate (UMP), cytidine 5'-monophosphate (CMP), and inosine 5'-monophosphate (IMP) in infant formula and adult/pediatric nutritional formula. It uses hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC-MS/MS). Preparation of the internal standards was conducted using centrifugal ultrafiltration and the standards are AMP- (13)C10, (15)N5; GMP-(13)C10, (15)N5; UMP-(13)C9, (15)N2; and15 CMP- (13)C9, (15)N3. Data were collected by using multiple reaction monitoring of the product ions of protonated molecules of the five nucleotides generated by positive-electrospray ionization. The HILIC conditions were conducted with ammonium formate (30 mmol/L) in water (pH 2.5, adjusted with formic acid) and methanol. The LOD and LOQ of the standard solution were 0.005-0.01 and 0.01-0.03 microg/mL, respectively. Recovery data were collected for intraday and interday testing and ranged from 98.1 to 108.9% with an RSD of 0.7-5.4%. The analytical range of the method is between 0.04 to 5 microg/mL for standard solution.", "gemini": "Phương pháp chính thức 2011.21 dùng để định lượng các nucleotide sau: adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP), uridine 5'-monophosphate (UMP), cytidine 5'-monophosphate (CMP) và inosine 5'-monophosphate (IMP) trong sữa công thức dành cho trẻ sơ sinh và sữa công thức dinh dưỡng dành cho người lớn/trẻ em. Phương pháp này sử dụng sắc ký lỏng tương tác ưa nước với phép đo phổ khối song song (HILIC-MS/MS). Việc chuẩn bị các chuẩn nội được tiến hành bằng cách sử dụng siêu lọc ly tâm và các chuẩn là AMP- (13)C10, (15)N5; GMP-(13)C10, (15)N5; UMP-(13)C9, (15)N2; và15 CMP- (13)C9, (15)N3. Dữ liệu được thu thập bằng cách sử dụng giám sát phản ứng đa của các ion sản phẩm của các phân tử có proton của năm nucleotide được tạo ra bởi ion hóa điện phun dương. Các điều kiện HILIC được tiến hành với amoni formiat (30 mmol/L) trong nước (pH 2,5, điều chỉnh bằng axit formic) và metanol. LOD và LOQ của dung dịch chuẩn lần lượt là 0,005-0,01 và 0,01-0,03 microg/mL. Dữ liệu thu hồi được thu thập cho thử nghiệm trong ngày và giữa các ngày và dao động từ 98,1 đến 108,9% với RSD là 0,7-5,4%. Phạm vi phân tích của phương pháp nằm trong khoảng từ 0,04 đến 5 microg/mL đối với dung dịch chuẩn."} +{"idx": 1980008, "en": "With the development of methods for obtaining chiral compounds as potential drugs, there is also need to develop analytical methods for the separation of both enantiomers. Keeping in mind that the physical and chemical properties of both enantiomers are identical, their different nature will only be revealed in a chiral environment that is appropriately designed. Physicochemical systems can be used to predict the differences in biological activity of both enantiomers. The complexity of the problem requires the use of additional tools, which are various chemometric methods. This paper reviews the application of chemometry in the analysis of chiral drugs and discusses the effects of a combination of chromatographic, electrophoretic, and spectroscopic analysis (UV-Vis absorption spectroscopy, and near-IR spectroscopy aided by cyclodextrin inclusion complexes) with chemometrics for improving the methods of enantioseparation (experimental design), explaining the mechanisms of behavior and chiral recognition (quantitative structure-enantioselective retention relationships) and indicating chiral purity (enantiomeric excess).", "gemini": "Với sự phát triển của các phương pháp để thu được các hợp chất chiral là thuốc tiềm năng, cũng cần phát triển các phương pháp phân tích để tách cả hai đồng phân đối quang. Cần lưu ý rằng các tính chất vật lý và hóa học của cả hai đồng phân đối quang là giống hệt nhau, bản chất khác nhau của chúng sẽ chỉ được bộc lộ trong môi trường chiral được thiết kế phù hợp. Các hệ thống vật lý hóa học có thể được sử dụng để dự đoán sự khác biệt về hoạt tính sinh học của cả hai đồng phân đối quang. Tính phức tạp của vấn đề đòi hỏi phải sử dụng các công cụ bổ sung, đó là các phương pháp hóa học khác nhau. Bài báo này xem xét ứng dụng của hóa học trong phân tích thuốc chiral và thảo luận về tác động của sự kết hợp giữa phân tích sắc ký, điện di và quang phổ (phổ hấp thụ UV-Vis và phổ hồng ngoại gần được hỗ trợ bởi các phức hợp bao gồm cyclodextrin) với hóa học để cải thiện các phương pháp phân tách đối quang (thiết kế thử nghiệm), giải thích các cơ chế hành vi và nhận dạng chiral (mối quan hệ giữ lại chọn lọc cấu trúc định lượng) và chỉ ra độ tinh khiết quang học (thặng dư đồng phân)."} +{"idx": 1980009, "en": "A quantitative structure-activity relationship (QSAR) relates quantitative chemical structure attributes (molecular descriptors) to a biological activity. QSAR studies have now become attractive in drug discovery and development because their application can save substantial time and human resources. Several parameters are important in the prediction ability of a QSAR model. On the one hand, different statistical methods may be applied to check the linear or nonlinear behavior of a data set. On the other hand, feature selection techniques are applied to decrease the model complexity, to decrease the overfitting/overtraining risk, and to select the most important descriptors from the often more than 1000 calculated. The selected descriptors are then linked to a biological activity of the corresponding compound by means of a mathematical model. Different modeling techniques can be applied, some of which explicitly require a feature selection. A QSAR model can be useful in the design of new compounds with improved potency in the class under study. Only molecules with a predicted interesting activity will be synthesized. In the feature selection problem, a learning algorithm is faced with the problem of selecting a relevant subset of features upon which to focus attention, while ignoring the rest. Up to now, many feature selection techniques, such as genetic algorithms, forward selection, backward elimination, stepwise regression, and simulated annealing have been used extensively. Swarm intelligence optimizations, such as ant colony optimization and partial swarm optimization, which are feature selection techniques usually simulated based on animal and insect life behavior to find the shortest path between a food source and their nests, recently are also involved in QSAR studies. This review paper provides an overview of different feature selection techniques applied in QSAR modeling.", "gemini": "Mối quan hệ định lượng giữa cấu trúc và hoạt tính (QSAR) liên quan đến các thuộc tính cấu trúc hóa học định lượng (mô tả phân tử) với một hoạt tính sinh học. Các nghiên cứu QSAR hiện đã trở nên hấp dẫn trong việc khám phá và phát triển thuốc vì ứng dụng của chúng có thể tiết kiệm đáng kể thời gian và nguồn nhân lực. Một số thông số rất quan trọng trong khả năng dự đoán của mô hình QSAR. Một mặt, các phương pháp thống kê khác nhau có thể được áp dụng để kiểm tra hành vi tuyến tính hoặc phi tuyến tính của một tập dữ liệu. Mặt khác, các kỹ thuật chọn đặc trưng được áp dụng để giảm độ phức tạp của mô hình, giảm rủi ro quá mức/huấn luyện quá mức và chọn các mô tả quan trọng nhất từ hơn 1000 mô tả được tính toán. Các mô tả được chọn sau đó được liên kết với hoạt động sinh học của hợp chất tương ứng bằng phương tiện của một mô hình toán học. Có thể áp dụng các kỹ thuật lập mô hình khác nhau, một số kỹ thuật yêu cầu rõ ràng phải chọn đặc trưng. Mô hình QSAR có thể hữu ích trong việc thiết kế các hợp chất mới có hiệu lực được cải thiện trong nhóm đang nghiên cứu. Chỉ những phân tử có hoạt tính thú vị được dự đoán mới được tổng hợp. Trong vấn đề lựa chọn đặc trưng, thuật toán học máy phải đối mặt với vấn đề lựa chọn một tập hợp con các đặc trưng có liên quan để tập trung chú ý, trong khi bỏ qua các đặc trưng còn lại. Cho đến nay, nhiều kỹ thuật chọn đặc trưng, như thuật toán di truyền, chọn tiến, loại bỏ lùi, hồi quy từng bước và ủ mô phỏng đã được sử dụng rộng rãi. Các tối ưu hóa trí thông minh của đàn, chẳng hạn như tối ưu hóa đàn kiến và tối ưu hóa đàn cục bộ, là các kỹ thuật chọn đặc trưng thường được mô phỏng dựa trên hành vi của động vật và côn trùng để tìm đường ngắn nhất giữa nguồn thức ăn và tổ của chúng, gần đây cũng đã tham gia vào các nghiên cứu QSAR. Bài báo đánh giá này cung cấp tổng quan về các kỹ thuật chọn đặc trưng khác nhau được áp dụng trong mô hình hóa QSAR."} +{"idx": 1980010, "en": "The calibration of an analytical method is a very important part of its development, and only the proper statistical and chemometric evaluation of the results, together with understanding this process, allows good results. The purpose of this minireview is to call the reader's attention to the major problems in calibration: curvilinearity, heteroscedasticity, presence of outliers, transformation of results, and distribution and autocorrelation of residuals. The common misunderstandings and mistakes are emphasized to inform the reader. Additionally, the computational package \"quantchem\" for GNU R environment, allowing full and automatic calibration evaluation, is presented.", "gemini": "Việc hiệu chuẩn một phương pháp phân tích là một phần rất quan trọng trong quá trình phát triển của nó và chỉ có đánh giá thống kê và hóa học thích hợp đối với kết quả, cùng với việc hiểu quá trình này, mới cho phép có kết quả tốt. Mục đích của bài đánh giá nhỏ này là để thu hút sự chú ý của người đọc đến các vấn đề chính trong hiệu chuẩn: độ cong, độ dị phương sai, sự hiện diện của các giá trị ngoại lai, chuyển đổi kết quả và phân phối và tự tương quan của phần dư. Những hiểu lầm và sai lầm phổ biến được nhấn mạnh để thông báo cho người đọc. Ngoài ra, gói tính toán \"quantchem\" cho môi trường GNU R, cho phép đánh giá hiệu chuẩn đầy đủ và tự động, được trình bày."} +{"idx": 1980011, "en": "In recent times, there has been a growing interest in artificial neural networks, which are a rough simulation of the information processing ability of the human brain, as modern and vastly sophisticated computational techniques. This interest has also been reflected in the pharmaceutical sciences. This paper presents a review of articles on the subject of the application of neural networks as effective tools assisting the solution of various problems in science and the pharmaceutical industry, especially those characterized by multivariate and nonlinear dependencies. After a short description of theoretical background and practical basics concerning the computations performed by means of neural networks, the most important pharmaceutical applications of neural networks, with suitable references, are demonstrated. The huge role played by neural networks in pharmaceutical analysis, pharmaceutical technology, and searching for the relationships between the chemical structure and the properties of newly synthesized compounds as candidates for drugs is discussed.", "gemini": "Trong thời gian gần đây, đã có sự quan tâm ngày càng tăng đối với mạng nơ-ron nhân tạo, là một mô phỏng thô sơ về khả năng xử lý thông tin của bộ não con người, như các kỹ thuật tính toán hiện đại và vô cùng tinh vi. Sự quan tâm này cũng được phản ánh trong khoa học dược phẩm. Bài báo này trình bày một đánh giá về các bài báo về chủ đề ứng dụng mạng nơ-ron như các công cụ hiệu quả hỗ trợ giải quyết các vấn đề khác nhau trong khoa học và ngành công nghiệp dược phẩm, đặc biệt là các vấn đề được đặc trưng bởi các mối phụ thuộc đa biến và phi tuyến tính. Sau một mô tả ngắn về nền tảng lý thuyết và các kiến thức cơ bản thực tế liên quan đến các phép tính được thực hiện bằng mạng nơ-ron, các ứng dụng dược phẩm quan trọng nhất của mạng nơ-ron, với các tài liệu tham khảo phù hợp, được chứng minh. Vai trò to lớn của mạng nơ-ron trong phân tích dược phẩm, công nghệ dược phẩm và tìm kiếm mối quan hệ giữa cấu trúc hóa học và các tính chất của các hợp chất mới tổng hợp làm ứng cử viên cho thuốc được thảo luận."} +{"idx": 1980012, "en": "Chemometrics is the application of statistical and mathematical methods to analytical data to permit maximum collection and extraction of useful information. The utility of chemometric techniques as tools enabling multidimensional calibration of selected spectroscopic, electrochemical, and chromatographic methods is demonstrated. Application of this approach mainly for interpretation of UV-Vis and near-IR (NIR) spectra, as well as for data obtained by other instrumental methods, makes identification and quantitative analysis of active substances in complex mixtures possible, especially in the analysis of pharmaceutical preparations present in the market. Such analytical work is carried out by the use of advanced chemical instruments and data processing, which has led to a need for advanced methods to design experiments, calibrate instruments, and analyze the resulting data. The purpose of this review is to describe various chemometric methods in combination with UV-Vis spectrophotometry, NIR spectroscopy, fluorescence spectroscopy, electroanalysis, chromatographic separation, and flow-injection analysis for the analysis of drugs in pharmaceutical preparations. Theoretical and practical aspects are described with pharmaceutical examples of chemometric applications. This review will concentrate on gaining an understanding of how chemometrics can be useful in the modern analytical laboratory. A selection of the most challenging problems faced in pharmaceutical analysis is presented, the potential for chemometrics is considered, and some consequent implications for utilization are discussed. The reader can refer to the citations wherever appropriate.", "gemini": "Hóa trắc là ứng dụng của các phương pháp thống kê và toán học vào dữ liệu phân tích để cho phép thu thập và trích xuất tối đa thông tin hữu ích. Tiện ích của các kỹ thuật hóa trắc như các công cụ cho phép hiệu chuẩn đa chiều của các phương pháp quang phổ, điện hóa và sắc ký được lựa chọn đã được chứng minh. Việc áp dụng phương pháp tiếp cận này chủ yếu để giải thích phổ UV-Vis và cận hồng ngoại (NIR), cũng như đối với dữ liệu thu được bằng các phương pháp dụng cụ khác, giúp có thể định danh và phân tích định lượng các hoạt chất trong hỗn hợp phức tạp, đặc biệt là trong phân tích dược phẩm chế phẩm có mặt trên thị trường. Công việc phân tích như vậy được thực hiện bằng cách sử dụng các dụng cụ hóa học tiên tiến và xử lý dữ liệu, điều này dẫn đến nhu cầu về các phương pháp tiên tiến để thiết kế thí nghiệm, hiệu chuẩn dụng cụ và phân tích dữ liệu thu được. Mục đích của bài đánh giá này là mô tả các phương pháp hóa trắc khác nhau kết hợp với phép đo quang phổ UV-Vis, quang phổ NIR, quang phổ huỳnh quang, phân tích điện, tách sắc ký và phân tích dòng chảy để phân tích thuốc trong các chế phẩm dược phẩm. Các khía cạnh lý thuyết và thực tế được mô tả với các ví dụ về dược phẩm của các ứng dụng hóa trắc. Đánh giá này sẽ tập trung vào việc hiểu cách hóa trắc có thể hữu ích trong phòng thí nghiệm phân tích hiện đại. Một số vấn đề thách thức nhất gặp phải trong phân tích dược phẩm được trình bày, tiềm năng của hóa trắc được xem xét và một số hệ quả liên quan đến việc sử dụng được thảo luận. Người đọc có thể tham khảo các trích dẫn bất cứ khi nào thấy phù hợp."} +{"idx": 1980013, "en": "This paper presents an assessment of the possibility of using topological indexes and related structural descriptors in TLC, HPLC, GC, micellar electrokinetic capillary chromatography (MEKC), and capillary zone electrophoresis (CZE) techniques. Every work quoted is a new aspect of the use of topological indexes. Data from the scientific literature indicate that the topological indexes discussed can be used to predict and describe retention and lipophilic parameters obtained by TLC, HPLC, GC, MEKC, and CZE for different classes of organic compounds including drugs. The results presented indicate that the topological indexes and other structural descriptors have significance in analytical investigations of organic compounds. This review indicates that further investigations on the application of the topological indexes in TLC, HPLC, GC, MEKC, and CZE are justifiable.", "gemini": "Bài báo này trình bày đánh giá về khả năng sử dụng các chỉ số tôpô và các mô tả cấu trúc liên quan trong các kỹ thuật TLC, HPLC, GC, sắc ký mao quản điện động micelle (MEKC) và sắc ký điện động mao quản vùng (CZE). Mỗi công trình được trích dẫn là một khía cạnh mới của việc sử dụng các chỉ số tôpô. Dữ liệu từ các tài liệu khoa học chỉ ra rằng các chỉ số tôpô được thảo luận có thể được sử dụng để dự đoán và mô tả các thông số giữ lại và ưa dầu thu được bằng TLC, HPLC, GC, MEKC và CZE đối với các lớp hợp chất hữu cơ khác nhau bao gồm cả thuốc. Các kết quả trình bày cho thấy các chỉ số tôpô và các mô tả cấu trúc khác có ý nghĩa trong các nghiên cứu phân tích các hợp chất hữu cơ. Đánh giá này chỉ ra rằng các nghiên cứu sâu hơn về ứng dụng của các chỉ số tôpô trong TLC, HPLC, GC, MEKC và CZE là hợp lý."} +{"idx": 1980014, "en": "This paper presents a preliminary study in building discriminant models from solid-state NMR spectrometry data to detect the presence of acetaminophen in over-the-counter pharmaceutical formulations. The dataset, containing 11 spectra of pure substances and 21 spectra of various formulations, was processed by partial least squares discriminant analysis (PLS-DA). The model found coped with the discrimination, and its quality parameters were acceptable. It was found that standard normal variate preprocessing had almost no influence on unsupervised investigation of the dataset. The influence of variable selection with the uninformative variable elimination by PLS method was studied, reducing the dataset from 7601 variables to around 300 informative variables, but not improving the model performance. The results showed the possibility to construct well-working PLS-DA models from such small datasets without a full experimental design.", "gemini": "Bài báo này trình bày một nghiên cứu sơ bộ về việc xây dựng các mô hình phân biệt từ dữ liệu phổ NMR trạng thái rắn để phát hiện sự hiện diện của acetaminophen trong các chế phẩm dược phẩm không kê đơn. Bộ dữ liệu, bao gồm 11 phổ của các chất tinh khiết và 21 phổ của các chế phẩm khác nhau, đã được xử lý bằng phân tích phân biệt bình phương cực tiểu một phần (PLS-DA). Mô hình được tìm thấy đã đối phó với sự phân biệt và các thông số chất lượng của nó là chấp nhận được. Người ta thấy rằng tiền xử lý biến chuẩn chuẩn gần như không có ảnh hưởng đến quá trình điều tra không giám sát đối với bộ dữ liệu. Ảnh hưởng của việc lựa chọn biến với việc loại bỏ biến không thông tin bằng phương pháp PLS đã được nghiên cứu, giảm bộ dữ liệu từ 7601 biến xuống còn khoảng 300 biến thông tin, nhưng không cải thiện hiệu suất của mô hình. Kết quả cho thấy khả năng xây dựng các mô hình PLS-DA hoạt động tốt từ các bộ dữ liệu nhỏ như vậy mà không cần thiết kế thí nghiệm đầy đủ."} +{"idx": 1980015, "en": "The photodegradation of moclobemide was studied in methanolic media. Ultra-HPLC (UHPLC)/MS/MS analysis proved decomposition to 4-chlorobenzamide as a major degradation product and small amounts of Ro 16-3177 (4-chloro-N-[2-[(2-hydroxyethyl)amino] ethyl]benzamide) and 2-[(4-chlorobenzylidene)amino]-N-[2-ethoxyethenyl]ethenamine. The methanolic solution was investigated spectrophotometrically in the UV region, registering the spectra during 30 min of degradation. Using reference spectra and a multivariate chemometric method (multivariate curve resolution-alternating least squares), the spectra were resolved and concentration profiles were obtained. The obtained results were in good agreement with a quantitative approach, with UHPLC-diode array detection as the reference method.", "gemini": "Sự phân hủy quang của moclobemide đã được nghiên cứu trong môi trường metanol. Phân tích UHPLC (UHPLC)/MS/MS chứng minh sự phân hủy thành 4-clorobenzamit là sản phẩm phân hủy chính và một lượng nhỏ Ro 16-3177 (4-clo-N-[2-[(2-hydroxyethyl)amino]etyl]benzamit) và 2-[(4-clobenzylidene)amino]-N-[2-etoxyethenyl]ethenamin. Dung dịch metanol được nghiên cứu bằng phương pháp quang phổ trong vùng UV, ghi phổ trong 30 phút phân hủy. Sử dụng phổ tham chiếu và phương pháp hóa học đa biến (phân giải đường cong đa biến-bình phương tối thiểu luân phiên), các phổ được phân giải và thu được các hồ sơ nồng độ. Các kết quả thu được phù hợp tốt với phương pháp định lượng, với phương pháp dò mảng điốt UHPLC làm phương pháp tham chiếu."} +{"idx": 1980016, "en": "A reflectance near-infrared (RNIR) spectroscopy method was developed for simultaneous determination of chondroitin (CH), glucosamine (GO), and ascorbic acid (AS) in capsule powder. A simple preparation of the sample was done by grinding, sieving, and compression of the powder sample for improving RNIR spectra. Partial least squares (PLS-1 and PLS-2) was successfully applied to quantify the three components in the studied mixture using information included in RNIR spectra in the 4240-9780 cm(-1) range. The calibration model was developed with the three drug concentrations ranging from 50 to 150% of the labeled amount. The calibration models using pure standards were evaluated by internal validation, cross-validation, and external validation using synthetic and pharmaceutical preparations. The proposed method was applied for analysis of two pharmaceutical products. Both pharmaceutical products had the same active principle and similar excipients, but with different nominal concentration values. The results of the proposed method were compared with the results of a pharmacopoeial method for the same pharmaceutical products. No significant differences between the results were found. The standard error of prediction was 0.004 for CH, 0.003 for GO, and 0.005 for AS. The correlation coefficient was 0.9998 for CH, 0.9999 for GO, and 0.9997 for AS. The highly accurate and precise RNIR method can be used for QC of pharmaceutical products.", "gemini": "Một phương pháp quang phổ phản xạ gần hồng ngoại (RNIR) đã được phát triển để xác định đồng thời chondroitin (CH), glucosamin (GO) và axit ascorbic (AS) trong bột viên nang. Một cách chế biến mẫu đơn giản được thực hiện bằng cách nghiền, rây và nén mẫu bột để cải thiện phổ RNIR. Bình phương tối thiểu từng phần (PLS-1 và PLS-2) đã được áp dụng thành công để định lượng ba thành phần trong hỗn hợp được nghiên cứu bằng cách sử dụng thông tin có trong phổ RNIR trong phạm vi 4240-9780 cm(-1). Mô hình hiệu chuẩn được phát triển với ba nồng độ thuốc trong khoảng từ 50 đến 150% lượng ghi nhãn. Các mô hình hiệu chuẩn sử dụng các tiêu chuẩn tinh khiết đã được đánh giá bằng cách xác nhận nội bộ, xác nhận chéo và xác nhận bên ngoài bằng cách sử dụng các chế phẩm tổng hợp và dược phẩm. Phương pháp đề xuất được áp dụng để phân tích hai sản phẩm dược phẩm. Cả hai sản phẩm dược phẩm đều có cùng nguyên tắc hoạt động và tá dược tương tự, nhưng có giá trị nồng độ danh nghĩa khác nhau. Kết quả của phương pháp đề xuất đã được so sánh với kết quả của phương pháp dược điển đối với cùng các sản phẩm dược phẩm đó. Không tìm thấy sự khác biệt đáng kể giữa các kết quả. Sai số chuẩn của dự đoán là 0,004 đối với CH, 0,003 đối với GO và 0,005 đối với AS. Hệ số tương quan là 0,9998 đối với CH, 0,9999 đối với GO và 0,9997 đối với AS. Phương pháp RNIR có độ chính xác và chính xác cao có thể được sử dụng để kiểm soát chất lượng các sản phẩm dược phẩm."} +{"idx": 1980017, "en": "Studies are constantly being conducted on the elaboration of efficient methods to confirm the compatibility of active pharmaceutical ingredients (APIs) and excipients, since medicinal products, apart from their APIs, also contain numerous excipients that not only have important functions in pharmaceutical preparations but can also initiate or participate in interactions with drug substances, which eventually lead to a decline in drug quality. With this in mind, research was undertaken to evaluate two of the most often applied pattern recognition methods, hierarchical cluster analysis (HCA) and principal component analysis (PCA), as supporting techniques in the identification of potential physicochemical interactions that may occur during the preformulation of solid dosage forms. The investigation performed with the use of baclofen and selected excipients has shown that with thermogravimetric analysis, HCA and PCA fulfill their role as supporting techniques in the interpretation of the data obtained. Based on these methods, it is possible to detect incompatibilities between baclofen and excipients, and the data obtained concur strongly with the results of differential scanning calorimetry and IR spectrometry analyses.", "gemini": "Các nghiên cứu liên tục được tiến hành về việc xây dựng các phương pháp hiệu quả để xác nhận tính tương thích của các thành phần dược phẩm hoạt tính (API) và tá dược, vì các sản phẩm thuốc, ngoài API của chúng, còn chứa nhiều tá dược không chỉ có các chức năng quan trọng trong các chế phẩm dược phẩm mà còn có thể khởi phát hoặc tham gia vào các tương tác với các chất thuốc, cuối cùng dẫn đến chất lượng thuốc giảm. Với mục đích này, nghiên cứu đã được tiến hành để đánh giá hai phương pháp nhận dạng mẫu được áp dụng thường xuyên nhất, phân tích cụm phân cấp (HCA) và phân tích thành phần chính (PCA), như các kỹ thuật hỗ trợ trong việc xác định các tương tác vật lý hóa học tiềm ẩn có thể xảy ra trong quá trình tiền bào chế các dạng bào chế rắn. Cuộc điều tra thực hiện với việc sử dụng baclofen và các tá dược đã chọn đã chỉ ra rằng với phân tích nhiệt trọng, HCA và PCA hoàn thành vai trò của chúng như các kỹ thuật hỗ trợ trong việc giải thích dữ liệu thu được. Dựa trên các phương pháp này, có thể phát hiện ra sự không tương thích giữa baclofen và tá dược và dữ liệu thu được rất phù hợp với kết quả phân tích nhiệt lượng vi sai và quang phổ hồng ngoại."} +{"idx": 1980018, "en": "In this study, the RP-HPLC method was investigated for the separation of citalopram and its four impurities by use of statistical experimental design. Initially, the influence of different experimental conditions (buffer pH, flow rate, and column temperature) on the chromatographic behavior of citalopram and its four impurities was investigated by use of partial least squares regression (PLSR) and multilayer perceptron (MLP) artificial neural networks (ANNs) trained by back-propagation. The developed models and the corresponding response surface plots were used to select the optimal HPLC conditions, buffer pH 7.0, flow rate 1.0 mL/ min, and column temperature 25 degrees C, for an efficient separation of citalopram and its four impurities. The elaborated HPLC method was found to be linear, specific, sensitive, precise, accurate, and robust. Retention times of citalopram and its impurities, obtained with the developed HPLC method, and the computed molecular parameters of the examined compounds were used in a quantitative structure retention relationship (QSRR) study. The PLSR and ANN algorithms were applied for the development of the QSRR methods. The MLP-two layers-ANN-QSRR model with root mean square error of prediction 0.105 and r(2) (observed versus predicted) 0.978 was selected. Since many different reaction conditions are applied for the synthesis of citalopram, different impurities and degradation products can be formed. Therefore, the developed QSRR model can be extended to the prediction of the retention times with the other citalopram impurities, degradation products, and metabolites.", "gemini": "Trong nghiên cứu này, phương pháp RP-HPLC đã được nghiên cứu để tách citalopram và bốn tạp chất của nó bằng cách sử dụng thiết kế thí nghiệm thống kê. Ban đầu, ảnh hưởng của các điều kiện thử nghiệm khác nhau (pH đệm, tốc độ dòng chảy và nhiệt độ cột) đối với hành vi sắc ký của citalopram và bốn tạp chất của nó đã được nghiên cứu bằng cách sử dụng hồi quy bình phương toàn phần (PLSR) và mạng nơ-ron nhân tạo đa lớp nhận thức (MLP) được đào tạo bằng cách truyền ngược. Các mô hình đã phát triển và các biểu đồ bề mặt đáp ứng tương ứng đã được sử dụng để lựa chọn các điều kiện HPLC tối ưu, pH đệm 7,0, tốc độ dòng chảy 1,0 mL / phút và nhiệt độ cột 25 độ C, để tách hiệu quả citalopram và bốn tạp chất của nó. Phương pháp HPLC được xây dựng được tìm thấy là tuyến tính, đặc hiệu, nhạy, chính xác, chính xác và mạnh mẽ. Thời gian lưu của citalopram và các tạp chất của nó, thu được bằng phương pháp HPLC đã phát triển và các thông số phân tử được tính toán của các hợp chất được kiểm tra đã được sử dụng trong nghiên cứu mối quan hệ giữ lại cấu trúc định lượng (QSRR). Các thuật toán PLSR và ANN đã được áp dụng để phát triển các phương pháp QSRR. Mô hình MLP-hai lớp-ANN-QSRR với lỗi bình phương trung bình của dự đoán là 0,105 và r (2) (quan sát so với dự đoán) 0,978 đã được chọn. Vì nhiều điều kiện phản ứng khác nhau được áp dụng cho quá trình tổng hợp citalopram, nên có thể hình thành các tạp chất và sản phẩm phân hủy khác nhau. Do đó, mô hình QSRR đã phát triển có thể được mở rộng để dự đoán thời gian lưu với các tạp chất citalopram khác, sản phẩm phân hủy và chất chuyển hóa."} +{"idx": 1980019, "en": "The relationships between experimental and computational descriptors of antihistamine drugs were studied using principal component analysis (PCA). Empirical data came from UV and IR spectroscopic measurements. Nonempirical data, such as structural molecular descriptors and chromatographic data, were obtained from HyperChem software. Another objective was to test whether the parameters used as independent variables (nonempirical and empirical-spectroscopic) could lead to attaining classification similar to that developed on the basis of the chromatographic parameters. To arrive at the answer to the question, a matrix of 18x49 data, including HPLC and UV and IR spectroscopic data, together with molecular modeling studies, was evaluated by the PCA method. The obtained clusters of drugs were consistent with the drugs' chemical structure classification. Moreover, the PCA method applied to the HPLC retention data and structural descriptors allowed for classification of the drugs according to their pharmacological properties; hence it may potentially help limit the number of biological assays in the search for new drugs.", "gemini": "Các mối quan hệ giữa các mô tả tính toán và thực nghiệm của thuốc kháng histamine đã được nghiên cứu bằng cách sử dụng phân tích thành phần chính (PCA). Dữ liệu thực nghiệm đến từ các phép đo quang phổ UV và IR. Dữ liệu không thực nghiệm, chẳng hạn như các mô tả phân tử cấu trúc và dữ liệu sắc ký, thu được từ phần mềm HyperChem. Một mục tiêu khác là kiểm tra xem các thông số được sử dụng làm biến độc lập (không thực nghiệm và quang phổ thực nghiệm) có thể dẫn đến phân loại tương tự như phân loại được phát triển trên cơ sở các thông số sắc ký hay không. Để đi đến câu trả lời cho câu hỏi, một ma trận dữ liệu 18x49, bao gồm dữ liệu quang phổ HPLC và UV và IR, cùng với các nghiên cứu mô hình phân tử, đã được đánh giá bằng phương pháp PCA. Các nhóm thuốc thu được phù hợp với phân loại cấu trúc hóa học của thuốc. Hơn nữa, phương pháp PCA áp dụng cho dữ liệu lưu giữ HPLC và các mô tả cấu trúc cho phép phân loại các loại thuốc theo các đặc tính dược lý của chúng; do đó, nó có khả năng giúp hạn chế số lượng xét nghiệm sinh học trong quá trình tìm kiếm các loại thuốc mới."} +{"idx": 1980020, "en": "Fifteen essential oils of pharmaceutical grade were fingerprinted by five techniques: TLC, GC, HPLC, attenuated total reflectance FTIR spectroscopy, and differential scanning calorimetry (DSC). Denoising and baseline removal was found to be a crucial step for correct comparative analysis. Standardization of the signal was not necessary in the presented case; however, it should be considered and checked in each case. Due to small variance explained by first two principal components (below 50%) and outlying observations, the main analysis was performed by Euclidean dendrograms. It was found that almost all techniques besides DSC find real chemical similarities; however, DSC can be used as an additional tool. The similarities among the five techniques were also compared and discussed.", "gemini": "Mười lăm loại tinh dầu có dược tính đã được xác định bằng năm kỹ thuật: TLC, GC, HPLC, quang phổ hồng ngoại phản xạ toàn phần suy yếu và nhiệt lượng kế quét vi sai (DSC). Loại bỏ nhiễu và đường cơ sở được phát hiện là một bước quan trọng để phân tích so sánh chính xác. Việc chuẩn hóa tín hiệu là không cần thiết trong trường hợp được trình bày; tuy nhiên, nó nên được xem xét và kiểm tra trong từng trường hợp. Do phương sai nhỏ được giải thích bởi hai thành phần chính đầu tiên (dưới 50%) và các quan sát ngoại lai, nên phân tích chính được thực hiện bằng sơ đồ phân nhánh Euclid. Người ta thấy rằng hầu hết tất cả các kỹ thuật ngoài DSC đều tìm thấy những điểm tương đồng về mặt hóa học thực sự; tuy nhiên, DSC có thể được sử dụng như một công cụ bổ sung. Những điểm tương đồng giữa năm kỹ thuật cũng được so sánh và thảo luận."} +{"idx": 1980021, "en": "The quantification of prednisone in tablets was performed using partial least squares (PLS) models based on FTIR-attenuated total reflection (ATR) and FT-Raman spectra. To compare the predictive ability of these models, the relative standard error of prediction (RSEP) values were calculated. In the case of prednisone determination from the FT-Raman data, RSEP values of 3.1 and 3.2% for the calibration and validation data sets were obtained. For FTIR-ATR models, which were constructed using five spectra for each sample, these errors amounted to 2.6 and 2.9%, respectively. Four commercial products containing 1, 5, 10, and 20 mg prednisone/tablet were quantified. Concentrations derived from the elaborated models correlated strongly with the results of reference analyses and with the declared values (in parentheses). The analyses gave recoveries of 100.0-101.6% (100.1-103.0%) and 98.1-103.2% (100.4-102.9%) for FTIR-ATR and FT-Raman data, respectively. A successful quantification of prednisolone in tablets containing 5 mg active ingredient/tablet was also performed using the PLS model, which was based on FTIR-ATR spectra, with a recovery of 99.8 (98.8%). Both reported spectroscopic techniques can be used as fast and convenient alternatives to the standard pharmacopeial methods of prednisone and prednisolone quantification in solid dosage forms. However, in the case of FTIR-ATR spectroscopy, it is necessary to repeat measurements several times to obtain sufficiently low quantification errors.", "gemini": "Việc định lượng prednisone trong viên nén được thực hiện bằng cách sử dụng các mô hình bình phương cực tiểu một phần (PLS) dựa trên quang phổ phản xạ toàn phần suy yếu FTIR (ATR) và FT-Raman. Để so sánh khả năng dự đoán của các mô hình này, các giá trị sai số chuẩn tương đối của dự đoán (RSEP) đã được tính toán. Trong trường hợp xác định prednisone từ dữ liệu FT-Raman, các giá trị RSEP là 3,1 và 3,2% cho các tập dữ liệu hiệu chuẩn và xác thực đã thu được. Đối với các mô hình FTIR-ATR, được xây dựng bằng cách sử dụng năm phổ cho mỗi mẫu, các lỗi này lần lượt là 2,6 và 2,9%. Bốn sản phẩm thương mại chứa 1, 5, 10 và 20 mg prednisone/viên đã được định lượng. Nồng độ thu được từ các mô hình đã xây dựng có mối tương quan mạnh với kết quả phân tích tham chiếu và với các giá trị đã khai báo (trong ngoặc đơn). Các phân tích cho thấy tỷ lệ thu hồi là 100,0-101,6% (100,1-103,0%) và 98,1-103,2% (100,4-102,9%) đối với dữ liệu FTIR-ATR và FT-Raman. Việc định lượng thành công prednisolone trong viên nén chứa 5 mg hoạt chất/viên cũng được thực hiện bằng cách sử dụng mô hình PLS, dựa trên phổ FTIR-ATR, với tỷ lệ thu hồi là 99,8 (98,8%). Cả hai kỹ thuật quang phổ đã báo cáo đều có thể được sử dụng làm phương pháp thay thế nhanh chóng và tiện lợi cho các phương pháp dược điển chuẩn để định lượng prednisone và prednisolone trong các dạng bào chế rắn. Tuy nhiên, trong trường hợp quang phổ FTIR-ATR, cần phải lặp lại các phép đo nhiều lần để thu được các lỗi định lượng đủ thấp."} +{"idx": 1980022, "en": "This paper deals with the systematic comparison of extraction of major volatile aromatic compounds (VACs) of citronella grass and lemongrass by classical microhydrodistillation (MHD), as well as modern accelerated solvent extraction (ASE). Sixteen VACs were identified by GC/MS. GC-flame ionization detection was used for the quantification of five VACs (citronellal, citronellol, geraniol, citral, and eugenol) to compare the extraction efficiency of the two different methods. Linear range, LOD, and LOQ were calculated for the five VACs. Intraday and interday precisions for the analysis of VACs were determined for each sample. The extraction recovery, as calculated by a spiking experiment with known standards of VACs, by ASE and MHD ranged from 64.9 to 91.2% and 74.3 to 95.2%, respectively. The extraction efficiency of the VACs was compared for three solvents of varying polarities (hexane, dichloromethane, and methanol), seven different temperatures (ranging from 40 to 160 degrees C, with a gradual increment of 20 degrees C), five time periods (from 1 to 10 min), and three cycles (1, 2, and 3 repeated extractions). Optimum extraction yields of VACs were obtained when extractions were carried out for 7 min with dichloromethane and two extraction cycles at 120 degrees C. The results showed that the ASE technique is more efficient than MHD, as it results in improved yields and significant reduction in extraction time with automated extraction capabilities.", "gemini": "Bài báo này đề cập đến việc so sánh có hệ thống về việc chiết xuất các hợp chất thơm dễ bay hơi chính (VAC) của cỏ sả chanh và cỏ hương bài bằng phương pháp chưng cất vi mô cổ điển (MHD) cũng như phương pháp chiết xuất dung môi tăng tốc hiện đại (ASE). Mười sáu VAC đã được xác định bằng GC/MS. Phương pháp phát hiện ion hóa ngọn lửa GC đã được sử dụng để định lượng năm VAC (citronellal, citronellol, geraniol, citral và eugenol) để so sánh hiệu quả chiết xuất của hai phương pháp khác nhau. Phổ tuyến tính, LOD và LOQ đã được tính toán cho năm VAC. Độ chính xác trong ngày và giữa các ngày đối với việc phân tích VAC đã được xác định cho từng mẫu. Tỷ lệ thu hồi chiết xuất, được tính bằng thí nghiệm tăng cường với các tiêu chuẩn VAC đã biết, bằng ASE và MHD lần lượt trong khoảng từ 64,9 đến 91,2% và 74,3 đến 95,2%. Hiệu suất chiết xuất của VAC đã được so sánh đối với ba dung môi có độ phân cực khác nhau (hexan, dichloromethane và methanol), bảy nhiệt độ khác nhau (dao động từ 40 đến 160 độ C, tăng dần 20 độ C), năm khoảng thời gian (từ 1 đến 10 phút) và ba chu kỳ (1, 2 và 3 lần chiết xuất lặp lại). Năng suất chiết xuất VAC tối ưu thu được khi tiến hành chiết xuất trong 7 phút với dichloromethane và hai chu kỳ chiết xuất ở 120 độ C. Kết quả cho thấy kỹ thuật ASE hiệu quả hơn MHD, vì nó mang lại năng suất được cải thiện và giảm đáng kể thời gian chiết xuất với khả năng chiết xuất tự động."} +{"idx": 1980023, "en": "A new application of continuous wavelet transform (CWT) to overlapping peaks in a chromatogram was developed for the quantitative analysis of amiloride hydrochloride (AML) and hydrochlorothiazide (HCT) in tablets. Chromatographic analysis was done by using an ACQUITY ultra-performance LC (UPLC) BEH C18 column (50 x 2.1 mm id, 1.7 pm particle size) and a mobile phase consisting of methanol-0.1 M acetic acid (21 + 79, v/v) at a constant flow rate of 0.3 mL/min with diode array detection at 274 nm. The overlapping chromatographic peaks of the calibration set consisting of AML and HCT mixtures were recorded rapidly by using an ACQUITY UPLC H-Class system. The overlapping UPLC data vectors of AML and HCT drugs and their samples were processed by CWT signal processing methods. The calibration graphs for AML and HCT were computed from the relationship between concentration and areas of chromatographic CWT peaks. The applicability and validity of the improved UPLC-CWT approaches were confirmed by recovery studies and the standard addition technique. The proposed UPLC-CWT methods were applied to the determination of AML and HCT in tablets. The experimental results indicated that the suggested UPLC-CWT signal processing provides accurate and precise results for industrial QC and quantitative evaluation of AML-HCT tablets.", "gemini": "Một ứng dụng mới của biến đổi wavelet liên tục (CWT) đối với các đỉnh chồng lên nhau trong sắc ký đồ đã được phát triển để phân tích định lượng amilorid hydroclorid (AML) và hydrochlorothiazid (HCT) trong viên nén. Phân tích sắc ký được thực hiện bằng cách sử dụng cột ACQUITY ultra-performance LC (UPLC) BEH C18 (50 x 2,1 mm id, kích thước hạt 1,7 pm) và pha động gồm methanol-axit axetic 0,1 M (21 + 79, v/v) ở tốc độ dòng không đổi là 0,3 mL/phút với máy dò mảng diode ở bước sóng 274 nm. Các đỉnh sắc ký chồng lên nhau của bộ hiệu chuẩn gồm hỗn hợp AML và HCT được ghi lại nhanh chóng bằng cách sử dụng hệ thống ACQUITY UPLC H-Class. Các vectơ dữ liệu UPLC chồng lên nhau của thuốc AML và HCT và mẫu của chúng được xử lý bằng phương pháp xử lý tín hiệu CWT. Đồ thị hiệu chuẩn cho AML và HCT được tính toán từ mối quan hệ giữa nồng độ và diện tích các đỉnh CWT sắc ký. Khả năng áp dụng và tính hợp lệ của các phương pháp UPLC-CWT được cải tiến đã được xác nhận bằng các nghiên cứu thu hồi và kỹ thuật chuẩn độ. Các phương pháp UPLC-CWT được đề xuất đã được áp dụng để xác định AML và HCT trong viên nén. Kết quả thực nghiệm chỉ ra rằng phương pháp xử lý tín hiệu UPLC-CWT được đề xuất cung cấp kết quả chính xác và chính xác cho QC công nghiệp và đánh giá định lượng viên nén AML-HCT."} +{"idx": 1980024, "en": "Triterpenoid saponins are a class of glycosides with a wide range of bioactivities, which make them interesting research candidates. Zygophyllum coccineum is an Egyptian desert plant rich in triterpenoid saponins. Reviewing the relevant literature, no data concerning the HPLC or ultra-performance LC (UPLC) analysis of Zygophyllum content were found. This paper presents two methods, HPLC-UV and UPLC-UV-evaporative light scattering detector (ELSD)/MS, for the simultaneous determination of 10 compounds in the alcohol extract of Z. coccineum. The HPLC method uses a C18 column and water-acetonitrile (both containing 0.1% trifluoroacetic acid) gradient system. The separation was achieved within 32 min. The developed UPLC method simultaneously detects and quantifies the 10 compounds using an Acquity UPLC BEH Shield RP18 column and reagent alcohol-acetonitrile (80/20, v/v) and water (both containing 0.5% formic acid) gradient system within 14 min with UV, ELS, and MS detectors. The methods were used to analyze another species, Z. simplex, and results revealed a great variation between the secondary metabolite pattern of both species.", "gemini": "Saponin triterpenoid là một nhóm glycoside có nhiều hoạt tính sinh học, khiến chúng trở thành ứng cử viên nghiên cứu thú vị. Zygophyllum coccineum là một loại cây sa mạc của Ai Cập rất giàu saponin triterpenoid. Khi xem xét các tài liệu có liên quan, không tìm thấy dữ liệu nào liên quan đến phân tích HPLC hoặc LC hiệu suất siêu cao (UPLC) về hàm lượng Zygophyllum. Bài báo này trình bày hai phương pháp, HPLC-UV và UPLC-UV-máy dò tán xạ ánh sáng bay hơi (ELSD)/MS, để xác định đồng thời 10 hợp chất trong chiết xuất cồn của Z. coccineum. Phương pháp HPLC sử dụng cột C18 và hệ thống gradient nước-acetonitril (cả hai đều chứa 0,1% axit trifluoroaxetic). Phân tách đạt được trong vòng 32 phút. Phương pháp UPLC được phát triển phát hiện và định lượng đồng thời 10 hợp chất bằng cách sử dụng cột Acquity UPLC BEH Shield RP18 và hệ thống gradient phản ứng rượu-acetonitril (80/20, v/v) và nước (cả hai đều chứa 0,5% axit formic) trong vòng 14 phút với máy dò UV, ELS và MS. Các phương pháp này đã được sử dụng để phân tích một loài khác, Z. simplex, và kết quả cho thấy sự khác biệt lớn giữa kiểu chuyển hóa thứ cấp của cả hai loài."} +{"idx": 1980025, "en": "A fast, low-cost, convenient, and especially sensitive voltammetric screening approach for the study of the antioxidant properties of isoquercitrin and pedalitin from Pterogyne nitens is suggested in this work. These flavonoids were investigated for their redox properties using cyclic voltammetry in nonaqueous media using N,N-dimethylformamide and tetrabutylammonium tetrafluorborate as the supporting electrolyte, a glassy carbon working electrode, A6(see symbol in text)AgCI reference electrode, and Pt bare wire counter electrode. The comparative analysis of the activity of rutin has also been carried out. Moreover, combining HPLC with an electrochemical detector allowed qualitative and quantitative detection of micromolecules (e.g., isoquercitrin and pedalitin) that showed antioxidant activities. These results were then correlated to the inhibition of beta-carotene bleaching determined by TLC autographic assay and to structural features of the flavonoids.", "gemini": "Trong công trình này, một cách tiếp cận sàng lọc voltammetric nhanh chóng, chi phí thấp, thuận tiện và đặc biệt nhạy để nghiên cứu các đặc tính chống oxy hóa của isoquercitrin và pedalitin từ Pterogyne nitens đã được đề xuất. Các flavonoid này đã được nghiên cứu về tính chất oxy hóa khử của chúng bằng phương pháp voltammetry vòng trong môi trường không chứa nước sử dụng N,N-dimethylformamide và tetrabutylammonium tetrafluorborate làm chất điện phân hỗ trợ, điện cực làm việc bằng carbon thủy tinh, điện cực tham chiếu A6 (xem ký hiệu trong văn bản) AgCI và điện cực đối diện bằng dây trần Pt. Phân tích so sánh hoạt tính của rutin cũng đã được thực hiện. Hơn nữa, việc kết hợp HPLC với máy dò điện hóa cho phép phát hiện định tính và định lượng các phân tử nhỏ (ví dụ: isoquercitrin và pedalitin) có hoạt tính chống oxy hóa. Những kết quả này sau đó được liên hệ với sự ức chế quá trình tẩy màu beta-caroten được xác định bằng phương pháp tự động TLC và với các đặc điểm cấu trúc của flavonoid."} +{"idx": 1980026, "en": "A new extraction method for the analysis of the volatile fraction of white and red wines has been developed and validated. A dehydration step with MgSO4 separated an aroma compounds-rich alcoholic-glycerine layer. Spiked samples showed good recoveries in the range between 75 and 120% with CVmax% of 17, except for 2-phenylethanol and y-butyrolactone for which recoveries in red wines were under 60%, and for monoethylsuccinate, where recoveries averaged 50 and 60% in white and red wines, respectively. Method repeatability and intermediate precision showed good CVmax% with minimum and maximum values between 7.7 and 24, and between 18.7 and 25.0, respectively. The average determination coefficients were greater than 0.99 with CVmax% of 13. The instrumental LOD and LOQ were, in all cases, under 0.05 mg/L, except for 2,3 butanediol (0.20 mg/L). Overall, the presence of wine matrix affected aroma compounds responses in GC/MS analysis. These observations indicate that the use of a matrix-matched calibration curve is mandatory to obtain reliable quantitative data.", "gemini": "Một phương pháp chiết xuất mới để phân tích phần dễ bay hơi của rượu vang trắng và đỏ đã được phát triển và xác nhận. Một bước mất nước với MgSO4 tách ra một lớp rượu-glycerin giàu hợp chất hương thơm. Các mẫu có pha thêm chất chuẩn cho thấy khả năng thu hồi tốt trong khoảng từ 75 đến 120% với CVmax% là 17, ngoại trừ 2-phenylethanol và y-butyrolactone mà khả năng thu hồi trong rượu vang đỏ dưới 60% và đối với monoethylsuccinate, khả năng thu hồi trung bình là 50 và 60% trong rượu vang trắng và đỏ. Độ lặp lại của phương pháp và độ chính xác trung gian cho thấy CVmax% tốt với các giá trị tối thiểu và tối đa lần lượt là 7,7 và 24 và 18,7 và 25,0. Hệ số xác định trung bình lớn hơn 0,99 với CVmax% là 13. LOD và LOQ của thiết bị, trong mọi trường hợp, đều dưới 0,05 mg/L, ngoại trừ 2,3 butanediol (0,20 mg/L). Nhìn chung, sự hiện diện của ma trận rượu ảnh hưởng đến phản ứng của các hợp chất hương thơm trong phân tích GC/MS. Những quan sát này chỉ ra rằng việc sử dụng đường chuẩn khớp với ma trận là bắt buộc để có được dữ liệu định lượng đáng tin cậy."} +{"idx": 1980027, "en": "A collaborative study was conducted to evaluate the performance of the VITEK 2 Gram-negative (GN) Identification card for use with the VITEK 2 automated microbial identification system. The GN test card is used in the identification of fermenting and nonfermenting Gram-negative bacilli, including the select agent organisms Brucella melitensis, Francisella tularensis, Burkholderia mallei, B. pseudomallei, and Yersinia pestis. The VITEK 2 GN card is based on 47 biochemical tests measuring carbon source utilization, inhibition and resistance, and enzymatic activities. A total of 20 laboratories representing government, industry, and private testing facilities throughout the United States participated. In this study, 720 Gram-negative inclusivity isolates were analyzed by the GN Identification method. Of the 720 well-characterized isolates, 707 were identified correctly, 0 were misidentified, 0 were unidentified, and 13 were not characterized as a Gram-negative organism. Additionally, 120 isolates exclusive of fermenting and nonfermenting Gram-negative bacilli were screened by Gram stain. A total of 117 isolates were correctly excluded. Three organisms were incorrectly characterized by Gram stain procedures, resulting in incorrect analysis and misidentification by VITEK 2 GN. The VITEK 2 GN identification method is an acceptable automated method for the rapid identification of Gram-negative bacteria.", "gemini": "Một nghiên cứu hợp tác đã được tiến hành để đánh giá hiệu suất của thẻ định danh VITEK 2 Gram âm (GN) để sử dụng với hệ thống định danh vi sinh vật tự động VITEK 2. Thẻ thử GN được sử dụng để định danh trực khuẩn Gram âm lên men và không lên men, bao gồm cả các tác nhân chọn lọc Brucella melitensis, Francisella tularensis, Burkholderia mallei, B. pseudomallei và Yersinia pestis. Thẻ VITEK 2 GN dựa trên 47 xét nghiệm sinh hóa đo lường việc sử dụng nguồn carbon, ức chế và kháng thuốc, và hoạt động của enzym. Tổng cộng có 20 phòng thí nghiệm đại diện cho các cơ sở thử nghiệm của chính phủ, công nghiệp và tư nhân trên khắp Hoa Kỳ đã tham gia. Trong nghiên cứu này, 720 phân lập bao gồm Gram âm đã được phân tích bằng phương pháp định danh GN. Trong số 720 phân lập được đặc trưng tốt, 707 được định danh chính xác, 0 bị định danh sai, 0 không xác định và 13 không được đặc trưng là sinh vật Gram âm. Ngoài ra, 120 phân lập không bao gồm trực khuẩn Gram âm lên men và không lên men đã được sàng lọc bằng phương pháp nhuộm Gram. Tổng cộng có 117 phân lập đã được loại trừ chính xác. Ba sinh vật đã được đặc trưng không chính xác bằng các quy trình nhuộm Gram, dẫn đến phân tích không chính xác và định danh sai bằng VITEK 2 GN. Phương pháp định danh VITEK 2 GN là một phương pháp tự động được chấp nhận để định danh nhanh vi khuẩn Gram âm."} +{"idx": 1980028, "en": "European Council Regulation 104/2000 states that fishery products must be labeled to indicate commercial designation of species, the production method, and the catch area. Therefore, traceability of seafood implies knowledge of the species offered to retail and their origin. Ensis siliqua is a bivalve intensively fished in Europe and sold in fresh and canned forms. Although several published methods clearly differentiate Ensis genus species, none of those assess the origin of the commercial samples. In the present study, a microsatellite marker (Esi-UDC3055F) was developed to establish the catch area of E. siliqua samples. Amplification yielded a fragment of 275 or 302 base pairs, depending on whether they were Iberian or Irish populations. The usefulness of this method was also assessed in commercial samples. The results of this study provide a reliable methodology for the identification of catch area in European E. siliqua commercial samples. The coupling of this methodology with existing techniques for razor clam species identification provides a powerful tool for traceability and labeling enforcement.", "gemini": "Quy định 104/2000 của Hội đồng Châu Âu nêu rõ rằng các sản phẩm thủy sản phải được dán nhãn để chỉ định tên thương mại của loài, phương pháp sản xuất và khu vực đánh bắt. Do đó, khả năng truy xuất nguồn gốc của hải sản ngụ ý kiến thức về các loài được bán lẻ và nguồn gốc của chúng. Ensis siliqua là một loài nhuyễn thể hai mảnh vỏ được đánh bắt nhiều ở Châu Âu và được bán dưới dạng tươi và đóng hộp. Mặc dù một số phương pháp đã công bố phân biệt rõ ràng các loài thuộc chi Ensis, nhưng không có phương pháp nào đánh giá nguồn gốc của các mẫu thương mại. Trong nghiên cứu hiện tại, một dấu hiệu vi vệ tinh (Esi-UDC3055F) đã được phát triển để thiết lập khu vực đánh bắt của các mẫu E. siliqua. Sự khuếch đại tạo ra một đoạn 275 hoặc 302 cặp bazơ, tùy thuộc vào việc chúng là quần thể Iberia hay Ireland. Tính hữu dụng của phương pháp này cũng đã được đánh giá trong các mẫu thương mại. Kết quả của nghiên cứu này cung cấp một phương pháp đáng tin cậy để xác định khu vực đánh bắt trong các mẫu thương mại E. siliqua của Châu Âu. Việc kết hợp phương pháp này với các kỹ thuật hiện có để xác định loài nghêu dao cạo cung cấp một công cụ mạnh mẽ để truy xuất nguồn gốc và thực thi dán nhãn."} +{"idx": 1980029, "en": "The liverwort, Porella vernicosa complex produces a very hot tasting polygodial, a drimane-type sesquiterpene dialdehyde. The same compound has been isolated from two ferns, Thelypteris hispidula and Blechnum fluviatile, as well as from the higher plants Polygonum hydropiper, P. hydropiper f. purpurascens (Polygonaceae), Cinnamosma, Caspicodendron, Canella and Warburgia species (Canellaceae), and Pseudowintera colorata, Tasmannia lanceolata, Drimys and Zygogynum species (Winteraceae). In addition, the liverworts and higher plants which elaborate polygodial and its related pungent drimane dials contain a small amount of alpha-tocopherol, gamma-tocopherol or delta-tocotrienol. The present paper gives the results of a comparative study on the drimane-type sesquiterpenoids in some liverworts, ferns and higher plants, and the role of tocopherols in these plant groups.", "gemini": "Rêu tản Porella vernicosa phức hợp sản xuất một polygodial có vị rất cay, một dialdehyd sesquiterpen loại drimane. Hợp chất tương tự đã được phân lập từ hai loài dương xỉ, Thelypteris hispidula và Blechnum fluviatile, cũng như từ các loài thực vật bậc cao Polygonum hydropiper, P. hydropiper f. purpurascens (Polygonaceae), Cinnamosma, Caspicodendron, Canella và Warburgia (Canellaceae), và Pseudowintera colorata, Tasmannia lanceolata, Drimys và Zygogynum (Winteraceae). Ngoài ra, rêu tản và thực vật bậc cao tạo ra polygodial và các loại drimane cay liên quan của nó chứa một lượng nhỏ alpha-tocopherol, gamma-tocopherol hoặc delta-tocotrienol. Bài báo hiện tại đưa ra kết quả của một nghiên cứu so sánh về các sesquiterpenoid loại drimane trong một số loài rêu tản, dương xỉ và thực vật bậc cao, và vai trò của tocopherol trong các nhóm thực vật này."} +{"idx": 1980030, "en": "The determination of trace elements in food and soil samples by atomic absorption spectrometry was investigated. A coprecipitation procedure with holmium hydroxide was used for separation-preconcentration of trace elements. Trace amounts of copper(II), manganese(II), cobalt(II), nickel(ll), chromium(lll), iron(Ill), cadmium(ll), and lead(ll) ions were coprecipitated with holmium hydroxide in 2.0 M NaOH medium. The optimum conditions for the coprecipitation process were investigated for several commonly tested experimental parameters, such as amount of coprecipitant, effect of standing time, centrifugation rate and time, and sample volume. The precision, based on replicate analysis, was lower than 10% for the analytes. In order to verify the accuracy of the method, the certified reference materials BCR 141 R calcareous loam soil and CRM 025-050 soil were analyzed. The procedure was successfully applied for separation and preconcentration of the investigated ions in various food and soil samples. An amount of the solid samples was decomposed with 15 mL concentrated hydrochloric acid-concentrated nitric acid (3 + 1). The preconcentration procedure was then applied to the final solutions. The concentration of trace elements in samples was determined by atomic absorption spectrometry.", "gemini": "Đã nghiên cứu việc xác định các nguyên tố vi lượng trong mẫu thực phẩm và đất bằng phương pháp quang phổ hấp thụ nguyên tử. Một quy trình kết tủa đồng với hydroxit holmi được sử dụng để tách-làm giàu trước các nguyên tố vi lượng. Các lượng vi lượng của các ion đồng(II), mangan(II), coban(II), niken(ll), crom(lll), sắt(Ill), cadimi(ll) và chì(ll) đã được kết tủa đồng với hydroxit holmi trong môi trường NaOH 2,0 M. Các điều kiện tối ưu cho quá trình kết tủa đồng đã được nghiên cứu đối với một số thông số thử nghiệm thường được thử nghiệm, chẳng hạn như lượng chất kết tủa đồng, hiệu ứng của thời gian đứng, tốc độ và thời gian ly tâm và thể tích mẫu. Độ chính xác, dựa trên phân tích lặp lại, thấp hơn 10% đối với các chất phân tích. Để xác minh độ chính xác của phương pháp, các vật liệu tham chiếu được chứng nhận là đất mùn vôi BCR 141 R và đất CRM 025-050 đã được phân tích. Quy trình này đã được áp dụng thành công để tách và làm giàu trước các ion được nghiên cứu trong nhiều mẫu thực phẩm và đất khác nhau. Một lượng mẫu rắn đã được phân hủy bằng 15 mL axit clohydric cô đặc - axit nitric cô đặc (3 + 1). Sau đó, quy trình làm giàu trước được áp dụng cho các dung dịch cuối cùng. Nồng độ các nguyên tố vi lượng trong mẫu được xác định bằng phương pháp quang phổ hấp thụ nguyên tử."} +{"idx": 1980031, "en": "From the dichloromethane extract of Nepeta sorgerae, the isolation and structure elucidation are now reported of a new isopimarane diterpenoid, named sorgerolone, and two known triterpenoids, oleanolic acid and ursolic acid. Antioxidant activity of the extracts and the isolated terpenoids was determined by the DPPH free radical scavenging and lipid peroxidation inhibition (beta-carotene bleaching) methods. Anticholinesterase activity of the extracts and isolates was investigated by Ellman's method against AChE and BChE enzymes. Although the antioxidant activity results were low, the AChE enzyme inhibition of the extracts and terpenoids was very promising.", "gemini": "Từ chiết xuất dichloromethan của Nepeta sorgerae, hiện đã báo cáo việc phân lập và giải thích cấu trúc của một diterpenoid isopimarane mới, có tên là sorgerolone và hai triterpenoid đã biết, axit oleanolic và axit ursolic. Hoạt tính chống oxy hóa của các chiết xuất và terpenoid phân lập được xác định bằng phương pháp thu dọn gốc tự do DPPH và ức chế quá trình peroxy hóa lipid (tẩy trắng beta-caroten). Hoạt tính chống cholinesterase của các chiết xuất và phân lập đã được nghiên cứu bằng phương pháp Ellman đối với các enzyme AChE và BChE. Mặc dù kết quả hoạt tính chống oxy hóa thấp, nhưng ức chế enzyme AChE của các chiết xuất và terpenoid rất hứa hẹn."} +{"idx": 1980032, "en": "The SDIX RapidChek Listeria F.A.S.T. test system was validated against the U.S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) cultural reference method for the detection of Listeria species on stainless steel, plastic, rubber, and painted concrete. The SDIX method uses a proprietary RapidChek Listeria enrichment media for a one-step, 24-40 h enrichment at 30 degrees C, and detects Listeria on an immunochromatographic lateral flow device in 10 min. Different Listeria species were used to spike each of the environmental surfaces. Environmental surfaces were spiked at levels ranging from 50 to 400 CFU/surface (1 in.2 swabs for painted concrete, 4 in.(2) for sponge). A total of 120 spiked samples were tested by the SDIX method at 24 and 40 h and the cultural reference method. Total confirmed positives were 49, 54, and 48 for the SDIX 24 h method, the SDIX 40 h method, and the USDA-FSIS cultural reference method, respectively. Nonspiked samples from all environmental surfaces were reported as negative for Listeria spp. by all methods. The overall Chi square was 0.017 (P = 0.104) and 0.611 (P= 0.566) after a 24 and 40 h enrichment, respectively, indicating that the test method was equivalent in performance to the reference method at both enrichment times. The SDIX method was evaluated for the detection of 50 Listeria and 35 non-Listeria bacterial strains. All 50 Listeria strains were detected by the method (100% sensitivity). Five out of 35 non-Listeria species gave light test signals when grown in nonselective broth culture and tested undiluted. However, when grown in the RapidChek Listeria F.A.S.T. proprietary media, only one bacterial strain (Staphylococcus aureus) was detected, giving a very low test signal (97% specificity). The method was shown to be robust toward several alterations in testing and storage conditions.", "gemini": "Hệ thống xét nghiệm SDIX RapidChek Listeria F.A.S.T. đã được xác nhận so với phương pháp tham chiếu nuôi cấy của Bộ Nông nghiệp Hoa Kỳ - Cơ quan Kiểm tra và An toàn Thực phẩm (USDA-FSIS) để phát hiện các loài Listeria trên thép không gỉ, nhựa, cao su và bê tông sơn. Phương pháp SDIX sử dụng môi trường làm giàu Listeria RapidChek độc quyền để làm giàu một bước, 24-40 giờ ở 30 độ C và phát hiện Listeria trên thiết bị dòng chảy bên miễn dịch sắc ký trong 10 phút. Các loài Listeria khác nhau đã được sử dụng để tăng cường mỗi bề mặt môi trường. Các bề mặt môi trường được tăng cường ở các mức từ 50 đến 400 CFU/bề mặt (tăm bông 1 in.2 đối với bê tông sơn, 4 in.(2) đối với miếng bọt biển). Tổng cộng 120 mẫu tăng cường đã được thử nghiệm bằng phương pháp SDIX ở thời điểm 24 và 40 giờ và phương pháp tham chiếu nuôi cấy. Tổng số dương tính được xác nhận là 49, 54 và 48 đối với phương pháp SDIX 24 giờ, phương pháp SDIX 40 giờ và phương pháp tham chiếu nuôi cấy USDA-FSIS. Các mẫu không tăng cường từ tất cả các bề mặt môi trường đều được báo cáo là âm tính với Listeria spp. theo mọi phương pháp. Tổng số Chi bình phương là 0,017 (P = 0,104) và 0,611 (P = 0,566) sau khi làm giàu 24 và 40 giờ, tương ứng, chỉ ra rằng phương pháp thử tương đương về hiệu suất với phương pháp tham chiếu tại cả hai thời điểm làm giàu. Phương pháp SDIX đã được đánh giá để phát hiện 50 chủng vi khuẩn Listeria và 35 chủng không phải Listeria. Tất cả 50 chủng Listeria đều được phát hiện bằng phương pháp này (độ nhạy 100%). Năm trong số 35 loài không phải Listeria cho tín hiệu thử nghiệm nhẹ khi nuôi trong môi trường nuôi cấy không chọn lọc và thử nghiệm không pha loãng. Tuy nhiên, khi nuôi trong môi trường độc quyền RapidChek Listeria F.A.S.T., chỉ phát hiện được một chủng vi khuẩn (Staphylococcus aureus), cho tín hiệu thử nghiệm rất thấp (độ đặc hiệu 97%). Phương pháp này đã được chứng minh là mạnh mẽ đối với một số thay đổi về điều kiện thử nghiệm và bảo quản."} +{"idx": 1980033, "en": "New chemometric approaches based on the application of partial least squares (PLS) and principal component regression (PCR) algorithms with fractional wavelet transform (FWT) and continuous wavelet transform (CWT) are proposed for the spectrophotometric multicomponent determination of thiamine hydrochloride (B1), pyridoxine hydrochloride (B6), and lidocaine hydrochloride (LID) in ampules without any separation step. In this study PLS and PCR techniques were applied to the raw spectral data, FWT-coefficients, and FWT-CWT-coefficients. These calibration models were labeled as Raw-PLS and Raw-PCR, FWT-PLS and FWT-PCR, and FWT-CWT-PLS and FWT-CWT-PCR, respectively. A new ultra-performance liquid chromatographic (UPLC) method was developed for the comparison of the results obtained by applying the chemometric calibration methods. Chromatographic separation and determination of B1, B6, and LID in ampules were performed on an Acquity UPLC BEH C18 column (50x2.1 mm id, 1.7 pm particle size) using gradient elution with a mobile phase consisting of methanol and 0.01 M HCI at a constant flow rate of 0.6 mL/min. These combined chemometric calibrations and UPLC were validated by analyzing various ternary mixtures, B1, B6, and LID. The proposed chemometric approaches (signal processing-multivariate calibrations) and UPLC method were applied to the quantitative multicomponent analysis of marketed ampules containing the vitamins B1 and B6 with LID.", "gemini": "Các phương pháp hóa học mới dựa trên ứng dụng của thuật toán bình phương cực tiểu từng phần (PLS) và hồi quy thành phần chính (PCR) với biến đổi wavelet phân số (FWT) và biến đổi wavelet liên tục (CWT) được đề xuất để xác định nhiều thành phần bằng phương pháp quang phổ của thiamin hydroclorid (B1), pyridoxin hydroclorid (B6) và lidocain hydroclorid (LID) trong ống thuốc mà không cần bất kỳ bước tách nào. Trong nghiên cứu này, các kỹ thuật PLS và PCR đã được áp dụng cho dữ liệu phổ thô, hệ số FWT và hệ số FWT-CWT. Các mô hình hiệu chuẩn này được dán nhãn là Raw-PLS và Raw-PCR, FWT-PLS và FWT-PCR, và FWT-CWT-PLS và FWT-CWT-PCR. Một phương pháp sắc ký lỏng hiệu suất cực cao (UPLC) mới đã được phát triển để so sánh các kết quả thu được bằng cách áp dụng các phương pháp hiệu chuẩn hóa học. Phân tách sắc ký và xác định B1, B6 và LID trong ống thuốc được thực hiện trên cột Acquity UPLC BEH C18 (50x2,1 mm id, kích thước hạt 1,7 pm) sử dụng phương pháp giải elution gradient với pha động gồm methanol và 0,01 M HCl ở tốc độ dòng không đổi 0,6 mL/phút. Các hiệu chuẩn hóa học kết hợp và UPLC này đã được xác nhận bằng cách phân tích nhiều hỗn hợp ba thành phần, B1, B6 và LID. Các phương pháp hóa học được đề xuất (hiệu chuẩn đa biến xử lý tín hiệu) và phương pháp UPLC đã được áp dụng để phân tích định lượng nhiều thành phần của các ống thuốc được bán trên thị trường có chứa vitamin B1 và B6 với LID."} +{"idx": 1980034, "en": "A new conversional synthesis of the ABC ring system of taxoids from the C19-diterpenoid alkaloid lycoctonine was developed in 6 steps with 2% overall yield. The distinctive features of the conversion include pinacol rearrangement, enlargement of ring B, and opening of a four-membered ring.", "gemini": "Một phương pháp chuyển đổi mới của hệ vòng ABC của taxoid từ ancaloit diterpenoid C19 lycoctonine đã được phát triển trong 6 bước với tổng năng suất 2%. Các đặc điểm riêng biệt của quá trình chuyển đổi bao gồm sự sắp xếp lại pinacol, mở rộng vòng B và mở vòng bốn thành viên."} +{"idx": 1980035, "en": "A simple and sensitive method has been developed for preconcentration and determination of genistein in soybeans. This method is based on cloud point extraction (CPE) of genistein from soybeans using ethylene glycol monoalkyl ether (Genapol X-080) as a nonionic surfactant. The concentration of extracted genistein was determined by HPLC with a UV detector. Optimum experimental conditions were established. With 5% Genapol X-080 (v/v), a liquid/solid ratio of 25:1 mL/g, and ultrasonic-assisted extraction at 40 degrees C for 45 min, the extraction percentage of genistein reached its highest value. The preconcentration factor for genistein was about 16.5. The RSD for seven replicate measurements and the LOD were +/- 4.45% and 15.0 ng/mL, respectively. CPE is simple, inexpensive, and suitable for extraction of genistein from soybean. It uses environmentally friendly surfactants and offers a convenient alternative to more conventional extraction systems.", "gemini": "Một phương pháp đơn giản và nhạy đã được phát triển để cô lập trước và xác định genistein trong đậu nành. Phương pháp này dựa trên chiết xuất điểm đám mây (CPE) của genistein từ đậu nành sử dụng ete monoalkyl glycol (Genapol X-080) làm chất hoạt động bề mặt không ion. Nồng độ genistein được chiết xuất được xác định bằng HPLC với máy dò UV. Các điều kiện thử nghiệm tối ưu đã được thiết lập. Với 5% Genapol X-080 (v/v), tỷ lệ lỏng/rắn là 25:1 mL/g và chiết xuất bằng siêu âm ở 40 độ C trong 45 phút, tỷ lệ chiết xuất genistein đạt giá trị cao nhất. Hệ số cô lập trước cho genistein là khoảng 16,5. RSD cho bảy phép đo lặp lại và LOD lần lượt là +/- 4,45% và 15,0 ng/mL. CPE đơn giản, không tốn kém và phù hợp để chiết xuất genistein từ đậu nành. Nó sử dụng chất hoạt động bề mặt thân thiện với môi trường và cung cấp một phương pháp thay thế thuận tiện cho các hệ thống chiết xuất thông thường hơn."} +{"idx": 1980036, "en": "A simple method using LC/MS/MS was developed and validated to determine residues of malachite green (MG) and leucomalachite green (LMG) in fish fillet. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique was used to perform the sample preparation. The optimal extraction and cleanup conditions were established using an experimental design. The validation parameters obtained to determine both MG and LMG complied with the requirements established by regulatory agencies for the presence of such substances in fish, which establish that the method must attain, at least, a minimum required performance limit of 2 ng/g. The accuracy values ranged between 95 and 107%, and the precision values were lower than 11.2%. The method was used in the analysis of tilapia samples (n = 20) commercialized in Campinas, SP, Brazil. None of the samples presented detectable levels of MG or LMG residues.", "gemini": "Một phương pháp đơn giản sử dụng LC/MS/MS đã được phát triển và xác nhận để xác định dư lượng xanh malachite (MG) và xanh leucomalachite (LMG) trong phi lê cá. Một kỹ thuật QuEChERS (Nhanh, Dễ dàng, Rẻ tiền, Hiệu quả, Chắc chắn và An toàn) đã được sử dụng để thực hiện việc chuẩn bị mẫu. Các điều kiện chiết xuất và làm sạch tối ưu đã được thiết lập bằng cách sử dụng thiết kế thử nghiệm. Các thông số xác nhận thu được để xác định cả MG và LMG đều tuân thủ các yêu cầu do các cơ quan quản lý thiết lập về sự hiện diện của các chất như vậy trong cá, theo đó phương pháp phải đạt được ít nhất là giới hạn hiệu suất tối thiểu bắt buộc là 2 ng/g. Các giá trị độ chính xác dao động trong khoảng từ 95 đến 107% và các giá trị độ chính xác thấp hơn 11,2%. Phương pháp này đã được sử dụng trong quá trình phân tích các mẫu cá rô phi (n = 20) được bán trên thị trường tại Campinas, SP, Brazil. Không có mẫu nào có mức dư lượng MG hoặc LMG có thể phát hiện được."} +{"idx": 1980037, "en": "Antiproliferative bioassay-guided fractionation of the ethanol extract of the endemic Malagasy Rubiaceous plant Tarenna grevei led to the isolation of two new antiproliferative oxygenated oleanane triterpene saponins. The structures of the two active compounds were elucidated as 23-hydroxylongispinogenin 3-O-beta-D-glucopyranoside (1) and longispinogenin 3-O-beta-D-glucopyranosyl (1 --> 2)-beta-D-glucopyranoside (3) by analyses of their spectral data including 1D- and 2D-NMR spectroscopy and chemical evidence. Compounds 1 and 3 displayed moderate antiproliferative activity against the A2780 ovarian cancer cell line with IC50 values of 7.6 and 4 microM, respectively.", "gemini": "Phân đoạn theo hướng dẫn sinh học chống tăng sinh của chiết xuất ethanol từ cây Rubiaceous đặc hữu của Madagascar Tarenna grevei đã dẫn đến việc phân lập hai saponin triterpen oleanane oxy hóa chống tăng sinh mới. Cấu trúc của hai hợp chất hoạt tính được làm sáng tỏ là 23-hydroxylongispinogenin 3-O-beta-D-glucopyranoside (1) và longispinogenin 3-O-beta-D-glucopyranosyl (1 --> 2)-beta-D-glucopyranoside (3) bằng cách phân tích dữ liệu quang phổ của chúng bao gồm quang phổ NMR 1D và 2D và bằng chứng hóa học. Các hợp chất 1 và 3 cho thấy hoạt tính chống tăng sinh vừa phải đối với dòng tế bào ung thư buồng trứng A2780 với giá trị IC50 lần lượt là 7,6 và 4 microM."} +{"idx": 1980038, "en": "An approach based on the difference (deltaab = deltaa - deltab) between 1H NMR chemical shifts (deltaa, deltab) of the geminal protons of oxymethylene (H2-26) (delta(ab) = < 0.2 for 25R; delta(ab) = > 0.5 for 25S) is proposed for ascertaining 25R/25S orientation of the 27-methyl group for (22R)-spirostane-type steroidal sapogenins and steroidal saponins. These studies suggested the 25R-orientation of the 27-Me group for the steroidal saponins isolated by Temraz et al. from Tribulus alatus.", "gemini": "Một phương pháp tiếp cận dựa trên sự khác biệt (deltaab = deltaa - deltab) giữa các dịch chuyển hóa học NMR 1H (deltaa, deltab) của các proton geminal của oxymethylene (H2-26) (delta(ab) = < 0,2 đối với 25R; delta(ab) = > 0,5 đối với 25S) được đề xuất để xác định hướng 25R/25S của nhóm 27-methyl đối với sapogenin steroid và saponin steroid loại (22R)-spirostane. Các nghiên cứu này cho thấy hướng 25R của nhóm 27-Me đối với saponin steroid được Temraz và cộng sự phân lập từ Tribulus alatus."} +{"idx": 1980039, "en": "Thirty three C19-diterpenoid alkaloids, twenty-two prepared from known C19-diterpenoid alkaloids and eleven isolated from Aconitum and Delphinium spp. were evaluated for their cardiac activity in the isolated bullfrog heart assay. Among them, eleven compounds exhibited cardiac activity, with average rate of amplitude increase in the range of 16-118%. Compound 7, mesaconine (17), hypaconine (25), and beiwutinine (26) exhibited strong cardiac activities relative to the reference drug. The structure-activity relationship data acquired indicated that an alpha-hydroxyl group at C-15, a hydroxyl group at C-8, an alpha-methoxyl or hydroxyl group at C-1, and a secondary amine or N-methyl group in ring A are important structure features necessary for the cardiac activities of the aconitine-type C19-diterpenoid alkaloids without any ester groups. In addition, an alpha-hydroxyl group at C-3 is also helpful for the cardiac activity of these alkaloids.", "gemini": "Ba mươi ba ancaloit diterpenoid C19, hai mươi hai ancaloit diterpenoid C19 đã biết và mười một ancaloit được phân lập từ Aconitum và Delphinium spp. đã được đánh giá hoạt tính tim của chúng trong xét nghiệm tim ếch cô lập. Trong số đó, mười một hợp chất biểu hiện hoạt tính tim, với tốc độ trung bình tăng biên độ trong khoảng 16-118%. Hợp chất 7, mesaconin (17), hypaconin (25) và beiwutinine (26) biểu hiện hoạt tính tim mạnh so với thuốc tham chiếu. Dữ liệu mối quan hệ cấu trúc-hoạt động thu được chỉ ra rằng một nhóm alpha-hydroxyl ở C-15, một nhóm hydroxyl ở C-8, một nhóm alpha-methoxyl hoặc hydroxyl ở C-1 và một amin bậc hai hoặc nhóm N-methyl trong vòng A là các đặc điểm cấu trúc quan trọng cần thiết cho hoạt động tim của các ancaloit diterpenoid C19 loại aconitin mà không có bất kỳ nhóm este nào. Ngoài ra, một nhóm alpha-hydroxyl ở C-3 cũng có lợi cho hoạt động tim của các ancaloit này."} +{"idx": 1980040, "en": "An SPE method for selective separation-preconcentration of Cu(ll), Zn(II), Ni(II), and Fe(III) on multiwalled carbon nanotubes (MWCNTs) modified by glutaric dihydrazide prior to flame atomic absorption spectrometric determination was investigated. The adsorption was achieved quantitatively on MWCNTs at pH 5.0, and then the retained metal ions on the adsorbent were eluted with 1 M HNO3. The effects of analytical parameters including pH of the solution, eluent type, sample volume, and matrix ions were investigated for optimization of the presented procedure. The adsorption capacity of the adsorbent at optimum conditions was found to be 33.6, 29.2, 22.1, and 36.0 mg/g for Cu(ll), Zn(ll), Ni(ll), and Fe(lll), respectively. The LOD values of the method were 0.21, 0.11, 0.24, and 0.27 microg/L for Cu(ll), Zn(ll), Ni(ll), and Fe(lll), respectively. The RSDs were lower than 3.01%. The method was applied for the determination of analytes in soil, river water, and wastewater samples with satisfactory results.", "gemini": "Một phương pháp SPE để tách-làm giàu trước có chọn lọc Cu(ll), Zn(II), Ni(II) và Fe(III) trên các ống nano cacbon nhiều thành (MWCNT) được sửa đổi bằng glutaric dihydrazid trước khi xác định bằng phương pháp quang phổ hấp thụ nguyên tử ngọn lửa đã được nghiên cứu. Sự hấp phụ đạt được định lượng trên MWCNT ở pH 5,0, sau đó các ion kim loại được giữ lại trên chất hấp phụ được rửa giải bằng 1 M HNO3. Các hiệu ứng của các thông số phân tích bao gồm pH của dung dịch, loại dung môi rửa giải, thể tích mẫu và các ion ma trận đã được nghiên cứu để tối ưu hóa quy trình được trình bày. Khả năng hấp phụ của chất hấp phụ ở điều kiện tối ưu được tìm thấy là 33,6, 29,2, 22,1 và 36,0 mg/g đối với Cu(ll), Zn(ll), Ni(ll) và Fe(lll), tương ứng. Các giá trị LOD của phương pháp là 0,21, 0,11, 0,24 và 0,27 microg/L đối với Cu(ll), Zn(ll), Ni(ll) và Fe(lll), tương ứng. Các giá trị RSD thấp hơn 3,01%. Phương pháp này được áp dụng để xác định các chất phân tích trong đất, nước sông và mẫu nước thải với kết quả đạt yêu cầu."} +{"idx": 1980041, "en": "Treatment of a 7,17-seco-type C19-diterpenoid alkaloid (3), prepared from deltaline (8), with triethylamine in either DMF or TEG (triethylene glycol) at 120 degrees C provided two interesting compounds 6 and 7. The structures of compounds 3, 6, and 7 were established based on extensive interpretations of their 1D and 2D NMR data. Compound 6, a lycoctonine-type C19-diterpenoid alkaloid, can be transformed from alkaloid 3 via Grob fragmentation, Prins reaction, and intramolecular disproportionation. The mechanism of the formation of compound 6 was confirmed by deuteration experiments. Product 7 was formed through a pinacol-like rearrangement of alkaloid 3.", "gemini": "Xử lý ancaloit diterpenoid C19 loại 7,17-seco (3), được điều chế từ deltaline (8), với triethylamin trong DMF hoặc TEG (triethylene glycol) ở nhiệt độ 120 độ C tạo ra hai hợp chất thú vị 6 và 7. Cấu trúc của các hợp chất 3, 6 và 7 được thiết lập dựa trên các diễn giải mở rộng về dữ liệu NMR 1D và 2D của chúng. Hợp chất 6, một ancaloit diterpenoid C19 loại lycoctonine, có thể được chuyển hóa từ ancaloit 3 thông qua sự phân mảnh Grob, phản ứng Prins và sự phân hủy không cân đối trong phân tử. Cơ chế hình thành hợp chất 6 đã được xác nhận bằng các thí nghiệm đơteri hóa. Sản phẩm 7 được hình thành thông qua sự sắp xếp lại giống như pinacol của ancaloit 3."} +{"idx": 1980042, "en": "Bioassay-guided fractionation of the cytotoxic ethanol extract of Cryptocarya chinensis has led to the isolation of 11 compounds, including two phenanthroindolizidine alkaloids [(-)-antofine (1) and dehydroantofine (2)], five pavine alkaloids (3-7), and four proaporphine alkaloids (8-11). The structures of the isolated compounds were determined by means of NMR spectroscopic methods, and supported by HRMS and optical rotation data. Compounds 1 and 2 showed cytotoxic activity against four cancer cell lines, L1210, P388, A549, and HCT-8, with 1 being the most potent against A549 and HCT-8 with EC50 values of 0.002 and 0.001 microg/mL, respectively. In addition, 2 is first reported to exhibit significant anti-HIV activity.", "gemini": "Phân đoạn hướng dẫn sinh học của chiết xuất ethanol độc tế bào của Cryptocarya chinensis đã dẫn đến việc phân lập 11 hợp chất, bao gồm hai ancaloit phenanthroindolizidine [(-)-antofine (1) và dehydroantofine (2)], năm ancaloit pavine (3-7) và bốn ancaloit proaporphine (8-11). Cấu trúc của các hợp chất được phân lập được xác định bằng phương pháp quang phổ NMR và được hỗ trợ bởi dữ liệu HRMS và độ quay quang. Các hợp chất 1 và 2 cho thấy hoạt tính độc tế bào đối với bốn dòng tế bào ung thư, L1210, P388, A549 và HCT-8, trong đó 1 là hợp chất mạnh nhất đối với A549 và HCT-8 với giá trị EC50 lần lượt là 0,002 và 0,001 microg/mL. Ngoài ra, 2 là hợp chất đầu tiên được báo cáo có hoạt tính chống HIV đáng kể."} +{"idx": 1980043, "en": "A new indole alkaloid of the pyridocarbazole type, named 6N-hydroxy-olivacine, and two known compounds, 2N-oxide-olivacine and olivacine, were isolated from roots of Peschiera affinis. The structures of the compounds were determined by spectroscopic {IR and extensive NMR (COSY, HMQC, HMBCand NOESY)} and EIMS analysis.", "gemini": "Một ancaloit indole mới thuộc loại pyridocarbazole, được đặt tên là 6N-hydroxy-olivacine và hai hợp chất đã biết, 2N-oxide-olivacine và olivacine, đã được phân lập từ rễ của Peschiera affinis. Cấu trúc của các hợp chất được xác định bằng phương pháp quang phổ {IR và NMR mở rộng (COSY, HMQC, HMBC và NOESY)} và phân tích EIMS."} +{"idx": 1980044, "en": "A new indole alkaloid, 11-hydroxypolyneuridine, was isolated from Vinca major subsp. major L. and the known indole alkaloids vallesiachotamine and isovallesiachotamine from Vinca minor L. This is the first report on the alkaloids of both Vinca species growing in Turkey; vallesiachotamine and isovallesiachotamine were isolated from a Vinca species for the first time. V. minor may be considered as a new source for these two alkaloids due to their occurrence in high amount in the aerial parts of the plant. The alkaloid extracts of the two Vinca species were found to have high lipid peroxidation inhibitory and DPPH radical scavenging activities. Anticholinesterase activity of the extracts was also very strong.", "gemini": "Một ancaloit indole mới, 11-hydroxypolyneuridine, đã được phân lập từ Vinca major subsp. major L. và các ancaloit indole đã biết là vallesiachotamine và isovallesiachotamine từ Vinca minor L. Đây là báo cáo đầu tiên về ancaloit của cả hai loài Vinca phát triển ở Thổ Nhĩ Kỳ; vallesiachotamine và isovallesiachotamine đã được phân lập từ một loài Vinca lần đầu tiên. V. minor có thể được coi là một nguồn mới cho hai ancaloit này do chúng xuất hiện với hàm lượng cao trong các bộ phận trên không của cây. Chiết xuất ancaloit của hai loài Vinca được phát hiện có hoạt tính ức chế quá trình peroxy hóa lipid và hoạt tính chống gốc tự do DPPH cao. Hoạt tính chống cholinesterase của các chiết xuất cũng rất mạnh."} +{"idx": 1980045, "en": "19-OH-(-)-eburnamonine 1 is a new indole alkaloid isolated from Bonafusia macrocalyx. A natural derivative from (-)-eburnamonine for which absolute configuration and conformation has been determined by making use of residual dipolar couplings enhanced NMR, circular dichroism spectra and high-level computations.", "gemini": "19-OH-(-)-eburnamonine 1 là một loại ancaloit indole mới được phân lập từ Bonafusia macrocalyx. Một dẫn xuất tự nhiên từ (-)-eburnamonine cấu hình tuyệt đối và cấu dạng đã được xác định bằng cách sử dụng các phép ghép đôi lưỡng cực còn lại tăng cường NMR, phổ dị sắc tròn và các phép tính cấp cao."} +{"idx": 1980046, "en": "Five new nitrogenous compounds were isolated from the Malayan Alstonia angustifolia and their structures determined based on interpretation of spectroscopic data.", "gemini": "Năm hợp chất nitơ mới đã được phân lập từ Alstonia angustifolia của Malaysia và cấu trúc của chúng được xác định dựa trên diễn giải dữ liệu quang phổ."} +{"idx": 1980047, "en": "A synthesis of a new alkaloid-fullerene conjugate (1) is reported. The reaction was carried out by photoinduced [3+2] cycloaddition of the Alstonia indole alkaloid, 6,7-seco-angustilobine B (2), to fullerene[C60] (3) under aerobic conditions. The major monoaddition photoadduct (1) was characterized unambiguously by UV, IR, MALDI-TOFMS and NMR experiments. A mechanism highlighted by sequential photoinduced electron transfer andradical recombination pathways is also proposed. No significant enhancement in inhibition against M. tuberculosis H37Rv was observed for 1 compared with its parent compounds 2 and 3.", "gemini": "Một tổng hợp của một liên hợp ancaloit-fullerene mới (1) được báo cáo. Phản ứng được thực hiện bằng cách quang kích [3+2] cycloaddition của ancaloit indole Alstonia, 6,7-seco-angustilobine B (2), với fullerene [C60] (3) trong điều kiện hiếu khí. Sản phẩm quang cộng chính (1) được đặc trưng một cách rõ ràng bằng các thí nghiệm UV, IR, MALDI-TOFMS và NMR. Một cơ chế được làm nổi bật bởi quá trình truyền electron quang kích tuần tự và các con đường tái kết hợp gốc cũng được đề xuất. Không có sự tăng cường đáng kể nào trong việc ức chế M. tuberculosis H37Rv được quan sát thấy đối với 1 so với các hợp chất mẹ của nó là 2 và 3."} +{"idx": 1980048, "en": "We describe here the isolation of three flavone 6-C-glycosides from the leaves of Anthurium andraeanum, The two new flavones were identified through detailed spectroscopic analysis as 4\"'-(3,4-dimethoxycinnamoyl)-embinin (2) and 4\"'-ferruloyl-embinin (3).", "gemini": "Chúng tôi mô tả ở đây về việc phân lập ba flavone 6-C-glycosides từ lá của Anthurium andraeanum, Hai flavone mới được xác định thông qua phân tích quang phổ chi tiết là 4\"'-(3,4-dimethoxycinnamoyl)-embinin (2) và 4\"'-ferruloyl-embinin (3)."} +{"idx": 1980049, "en": "Chamomile (Matricaria chamomilla L.), tarragon (Artemisia dracunculus L.) and Mexican arnica (Heterotheca inuoides) are common compositae spices and herbs found in the US market. They contain flavonoids and hydroxycinnamates that are potentially beneficial to human health. A standardized LC-PDA-ESI/MS profiling method was used to identify 51 flavonoids and 17 hydroxycinnamates. Many of the identifications were confirmed with authentic standards or through references in the literature or the laboratory's database. More than half of the phenol compounds for each spice had not been previously reported. The phenolic profile can be used for plant authentication and to correlate with biological activities.", "gemini": "Hoa cúc (Matricaria chamomilla L.), ngải giấm (Artemisia dracunculus L.) và arnica Mexico (Heterotheca inuoides) là những loại gia vị và thảo mộc thông thường có mặt trên thị trường Hoa Kỳ. Chúng chứa flavonoid và hydroxycinnamat có khả năng có lợi cho sức khỏe con người. Một phương pháp lập hồ sơ LC-PDA-ESI / MS chuẩn hóa đã được sử dụng để xác định 51 flavonoid và 17 hydroxycinnamat. Nhiều bản sắc đã được xác nhận bằng các tiêu chuẩn xác thực hoặc thông qua các tài liệu tham khảo trong tài liệu hoặc cơ sở dữ liệu của phòng thí nghiệm. Hơn một nửa các hợp chất phenol cho mỗi loại gia vị trước đây chưa được báo cáo. Hồ sơ phenol có thể được sử dụng để xác thực thực vật và để tương quan với các hoạt động sinh học."} +{"idx": 1980050, "en": "A methanolic extract from aerial parts of Polygonum hyrcanicum (Polygonaceae) showed high activity against Trypanosoma brucei rhodesiense (IC50 = 3.7 microg/mL). Bioassay-guided fractionation of the extract resulted in isolation of cinnamoylphenethyl amides, including N-trans-caffeoyltyramine (1), N-trans-p-coumaroyltyramine (7), and N-trans-feruloyltyramine (8) as the main active constituents (IC50s ranging from 2.2 to 13.3 microM). Some structurally related, but less active compounds, such as cannabisin B (2), tyrosol (3), p-coumaric acid (4), ferulic acid (5), and N-cis-feruloyltyramine (6) were also identified, along with N-trans-3,4-dimethoxycinnamoyldopamine (9). Cytotoxicity of the active compounds in L6 cells was determined, and selectivity indices (SI) of 7.9 to 33.4 were calculated.", "gemini": "Một chiết xuất methanol từ các bộ phận trên không của Polygonum hyrcanicum (Polygonaceae) cho thấy hoạt tính cao chống lại Trypanosoma brucei rhodesiense (IC50 = 3,7 microg / mL). Phân đoạn hướng dẫn sinh học của chiết xuất dẫn đến việc phân lập các amit cinnamoylphenethyl, bao gồm N-trans-caffeoyltyramine (1), N-trans-p-coumaroyltyramine (7) và N-trans-feruloyltyramine (8) là các thành phần hoạt động chính (IC50 dao động từ 2,2 đến 13,3 microM). Một số hợp chất có cấu trúc liên quan, nhưng ít hoạt động hơn, chẳng hạn như cannabisin B (2), tyrosol (3), axit p-coumaric (4), axit ferulic (5) và N-cis-feruloyltyramine (6) cũng được xác định, cùng với N-trans-3,4-dimethoxycinnamoyldopamine (9). Độc tính tế bào của các hợp chất hoạt động trong tế bào L6 đã được xác định và các chỉ số chọn lọc (SI) từ 7,9 đến 33,4 đã được tính toán."} +{"idx": 1980051, "en": "Decoctions of the aerial parts of either Bupleurum rigidum or B. fruticescens are equally used in certain parts of Spain for the treatment of topical and musculoskeletal inflammations. In the present paper, their phytochemical profile and pharmacological value has been compared. After chromatographic and spectral analyses we could establish the presence of rutin and absence of chlorogenic acid in B. fruticescens, whilst the contrary applies to B. rigidum, providing a means to chemically differentiate extracts and dry materials from the two species. Their free radical scavenging and antiperoxidative activities were similar, with B. fruticescens being more active overall. The infusions of both Bupleurum species also showed similar anti-inflammatory activity when tested by NF-kappaB assay (40% and 42% at 60 microg x mL(-1)), as well as in a hexosaminidase exocytosis assay (30% at 50 microg x mL(-1)). Antimigratory effects on rat melanoma B16F10 showed significant activity for both infusions, with B. rigidum twice as potent as B. fruticescens, the activity of the latter not being fully explained by its content of rutin. Taking all these results together, we can conclude that, in the selected experimental models, there exist an in vitro bioequivalence of the infusions from both species, which is in agreement with the majority of ethnopharmacological reports.", "gemini": "Thuốc sắc từ phần trên không của Bupleurum rigidum hoặc B. fruticescens được sử dụng như nhau ở một số vùng của Tây Ban Nha để điều trị tình trạng viêm tại chỗ và cơ xương. Trong bài báo này, đặc điểm hóa học và giá trị dược lý của chúng đã được so sánh. Sau khi phân tích sắc ký và quang phổ, chúng tôi có thể xác định sự hiện diện của rutin và không có axit chlorogenic trong B. fruticescens, trong khi ngược lại đối với B. rigidum, cung cấp phương tiện để phân biệt chiết xuất và vật liệu khô về mặt hóa học từ hai loài. Các hoạt động chống gốc tự do và chống peroxy hóa của chúng là tương tự nhau, với B. fruticescens hoạt động mạnh hơn nhìn chung. Thuốc sắc của cả hai loài Bupleurum cũng cho thấy hoạt động chống viêm tương tự khi được thử nghiệm bằng xét nghiệm NF-kappaB (40% và 42% ở 60 microg x mL(-1)), cũng như trong xét nghiệm ngoại bào hexosaminidase (30% ở 50 microg x mL(-1)). Các tác dụng chống di trú trên khối u ác tính B16F10 ở chuột cho thấy hoạt động đáng kể đối với cả hai loại thuốc sắc, với B. rigidum mạnh gấp đôi B. fruticescens, hoạt động của B. fruticescens không được giải thích đầy đủ bằng hàm lượng rutin của nó. Tổng hợp tất cả các kết quả này, chúng ta có thể kết luận rằng, trong các mô hình thực nghiệm được chọn, có sự tương đương sinh học trong ống nghiệm của các loại thuốc sắc từ cả hai loài, điều này phù hợp với phần lớn các báo cáo dân tộc dược lý."} +{"idx": 1980052, "en": "Chemical investigation of the stem bark of Saraca indica has resulted in the isolation of a new lignan glycoside, saracoside, along with four known lignan glycosides lyoniside, icariside E3, (+)5'-methoxyisolarciresinol-9'-O-beta-D-glucopyranoside and nudiposide, and a phenolic glucopyranoside, 3,4,5-trimethoxyphenyl-beta-D-glucopyranoside, which has been isolated for the first time from this species. The isolated lignan glycosides exhibit potent DNA topoisomerase IB inhibitory activity.", "gemini": "Nghiên cứu hóa học về vỏ thân cây Saraca indica đã phân lập được một loại glycosid lignan mới, saracoside, cùng với bốn loại glycosid lignan đã biết là lyoniside, icariside E3, (+) 5'-methoxyisolarciresinol-9'-O-beta-D-glucopyranoside và nudiposide, và một loại glycosid phenolic glucopyranoside, 3,4,5-trimethoxyphenyl-beta-D-glucopyranoside, lần đầu tiên được phân lập từ loài này. Các glycosid lignan được phân lập thể hiện hoạt tính ức chế topoisomerase IB của DNA mạnh mẽ."} +{"idx": 1980053, "en": "The basidiomycete Lentinus strigellus was cultivated in three different culture media and the secondary metabolites produced under different culture conditions were isolated and identified. When cultivated in a liquid medium with peptone, L. strigellus afforded the benzopyrans, 2,2-dimethyl-6-methoxychroman-4-one, 4-hydroxy-2,2-dimethyl-6-methoxychromane and (3R,4S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. The indole alkaloid echinuline and the anthraquinone fiscione, both unprecedented for the genus Lentinus, were isolated from the mycelium of the fungus. When cultured in Czapek medium enriched with potato broth, the fungus afforded the same benzopyrans except (3S,4S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. Panepoxydone and isopanepoxydone were also isolated when the microorganism was grown in Czapek medium.", "gemini": "Nấm tản Lentinus strigellus được nuôi cấy trong ba môi trường nuôi cấy khác nhau và các chất chuyển hóa thứ cấp được sản xuất trong các điều kiện nuôi cấy khác nhau đã được phân lập và xác định. Khi được nuôi cấy trong môi trường lỏng có pepton, L. strigellus tạo ra các benzopyran, 2,2-dimethyl-6-methoxychroman-4-one, 4-hydroxy-2,2-dimethyl-6-methoxychromane và (3R, 4S) -3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. Alkaloid indole echinuline và anthraquinone fiscione, cả hai đều chưa từng có đối với chi Lentinus, được phân lập từ sợi nấm của nấm. Khi được nuôi cấy trong môi trường Czapek bổ sung nước dùng khoai tây, nấm tạo ra cùng các benzopyran ngoại trừ (3S, 4S) -3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. Panepoxydone và isopanepoxydone cũng được phân lập khi vi sinh vật được nuôi cấy trong môi trường Czapek."} +{"idx": 1980054, "en": "Phytochemical studies of the leaves and rhizomes of Paraboea pa niculata (Gesneriaceae) are reported for the first time. Three phenylethanoid glycosides were isolated and characterized as 3,4-dihydroxyphenethyl-(3\"-O-beta-D-apiofuranosyl)-beta-D-glucopyranoside, calceoralarioside E, and acteoside. These isolates exhibited weak cytotoxic activity against the K-562 cell line with a 50% of cell killing rate of 40.18 microM, 27.05 microM, and 27.24 microM, respectively. In the DPPH free radical scavenging assay, their IC50 values were determined as 75.89 microM, 25.00 microM, and 26.04 microM, respectively.", "gemini": "Các nghiên cứu hóa thực vật về lá và thân rễ của cây Paraboea paniculata (Gesneriaceae) được báo cáo lần đầu tiên. Ba loại glycosid phenylethanoid đã được phân lập và xác định là 3,4-dihydroxyphenethyl-(3\"-O-beta-D-apiofuranosyl)-beta-D-glucopyranoside, calceoralarioside E và acteoside. Các chất phân lập này thể hiện hoạt tính tế bào độc yếu đối với dòng tế bào K-562 với tỷ lệ tiêu diệt tế bào 50% lần lượt là 40,18 microM, 27,05 microM và 27,24 microM. Trong xét nghiệm dọn gốc tự do DPPH, giá trị IC50 của chúng được xác định lần lượt là 75,89 microM, 25,00 microM và 26,04 microM."} +{"idx": 1980055, "en": "Villarinol (1), a new alkenoyloxy alkenol metabolite, has been isolated from the dichloromethane extract of Villaria odorata, an endemic Rubiaceae Philippine plant, along with the known compounds stigmasterol and 4-hydroxybenzaldehyde. The structure of 1 was elucidated on the basis of spectroscopic and spectrometric studies. The extracts of V. odorata exhibited moderate inhibition against Mycobacterium tuberculosis H37Rv, based on the colorimetric microplate alamar blue assay.", "gemini": "Villarinol (1), một chất chuyển hóa alkenoyloxy alkenol mới, đã được phân lập từ chiết xuất dichloromethane của Villaria odorata, một loài thực vật đặc hữu của họ Rubiaceae ở Philippines, cùng với các hợp chất đã biết là stigmasterol và 4-hydroxybenzaldehyde. Cấu trúc của 1 được làm sáng tỏ trên cơ sở các nghiên cứu quang phổ và phổ. Chiết xuất của V. odorata cho thấy khả năng ức chế vừa phải đối với Mycobacterium tuberculosis H37Rv, dựa trên xét nghiệm alamar blue vi mô màu."} +{"idx": 1980056, "en": "Two new ceramides were isolated from the bulbs of Lepidaploa cotoneaster (Willd. ex Spreng.) H. Rob. [Vernonia cotoneaster (Willd. ex Spreng.) Less.)], in addition to germanicol, beta-sitosterol, stigmasterol, 3-beta-O-beta-D-glucopyranosyl-sitosterol, lupeol, lupeoyl acetate and tiliroside. The structures of the new compounds were elucidated by spectral techniques (MS, 1H NMR, 13C NMR, HSQC, HMBC, DEPT, and TOCSY) and were compared with data reported in literature, and were established as 2S*,2'R*,3S*,4R*,11E)-N-[2'-hydroxyhenicosanoyl]-2-amino-nonadec-11-ene-1,3,4-triol (1) and (2S*,2'R*,3S*,4R*,8E)-N-[2'-hydroxytricosanoyl]-2-amino-nonadec-8-ene-1,3,4-triol (2). To establish the structure and to locate the double bond, the methyl ester of the fatty acid and dimethyl disulfide (DMDS) derivatives were prepared for both ceramides.", "gemini": "Hai ceramide mới đã được phân lập từ củ của cây Lepidaploa cotoneaster (Willd. ex Spreng.) H. Rob. [Vernonia cotoneaster (Willd. ex Spreng.) Less.)], ngoài germanicol, beta-sitosterol, stigmasterol, 3-beta-O-beta-D-glucopyranosyl-sitosterol, lupeol, lupeoyl acetate và tiliroside. Cấu trúc của các hợp chất mới được làm sáng tỏ bằng các kỹ thuật quang phổ (MS, 1H NMR, 13C NMR, HSQC, HMBC, DEPT và TOCSY) và được so sánh với dữ liệu được báo cáo trong tài liệu, và được xác định là 2S*,2'R*,3S*,4R*,11E)-N-[2'-hydroxyhenicosanoyl]-2-amino-nonadec-11-ene-1,3,4-triol (1) và (2S*,2'R*,3S*,4R*,8E)-N-[2'-hydroxytricosanoyl]-2-amino-nonadec-8-ene-1,3,4-triol (2). Để thiết lập cấu trúc và xác định vị trí liên kết đôi, este metyl của axit béo và dẫn xuất dimethyl disulfide (DMDS) đã được chuẩn bị cho cả hai ceramide."} +{"idx": 1980057, "en": "From the seeds of Alisma orientale, cis-aconitic anhydride ethyl ester and cis-2,4,5-trihydroxycinnamic acid were isolated, together with nine known phenolic compounds and a megastigmane sesquiterpene. All compounds are reported for the first time from Alisma species.", "gemini": "Từ hạt của Alisma orientale, este ethyl của cis-aconitic anhydride và axit cis-2,4,5-trihydroxycinnamic đã được phân lập, cùng với chín hợp chất phenolic đã biết và một sesquiterpene megastigmane. Tất cả các hợp chất được báo cáo lần đầu tiên từ loài Alisma."} +{"idx": 1980058, "en": "Alpinia katsumadai Hayata (Zingiberaceae) has been used as an anti-emetic medicine and to treat gastric disorders in Oriental Medicine. Previous phytochemical investigations of this plant have resulted in the isolation of various diarylheptanoids, kavalactones, flavonoids, stilbenes, monoterpenes, and sesquiterpenes. Some of these compounds have antioxidant, anti-emetic, antiviral, and cytoprotective effects. This review paper discusses the structural characterization of the chemical constituents of A. katsumadai, as well as the biological activity of pure constituents of this plant material.", "gemini": "Alpinia katsumadai Hayata (Zingiberaceae) đã được sử dụng như một loại thuốc chống nôn và để điều trị các rối loạn dạ dày trong Đông y. Các cuộc điều tra về thực vật học trước đây của loại cây này đã dẫn đến việc phân lập ra nhiều loại diarylheptanoid, kavalactone, flavonoid, stilben, monoterpen và sesquiterpen. Một số hợp chất này có tác dụng chống oxy hóa, chống nôn, kháng vi-rút và bảo vệ tế bào. Bài báo tổng quan này thảo luận về đặc điểm cấu trúc của các thành phần hóa học của A. katsumadai, cũng như hoạt tính sinh học của các thành phần tinh khiết của vật liệu thực vật này."} +{"idx": 1980059, "en": "The measurement of nitric oxide in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells is used as a model for evaluating the anti-inflammatory or chemopreventive potential of substances. Thienodolin, isolated from a Streptomyces sp. derived from Chilean marine sediment, inhibited nitric oxide production in LPS-stimulated RAW 264.7 cells (IC50 = 17.2 +/- 1.2 microM). At both the mRNA and protein levels, inducible nitric oxide synthase (iNOS) was suppressed in a dose-dependent manner. Mitogen-activated protein kinases (MAPKs), one major upstream signaling pathway involved in the transcription of iNOS, were not affected by treatment of thienodolin. However, the compound blocked the degradation of IkappaBa resulting in inhibition of NF-kappaB p65 nuclear translocation, and inhibited the phosphorylation of signal transducers and activators of transcription 1 (STAT1) at Tyr701. This study supports further exploration of thienodolin as a potential therapeutic agent with a unique mechanistic activity.", "gemini": "Việc đo nitric oxide trong các tế bào RAW 264.7 được kích thích bởi lipopolysaccharide (LPS) được sử dụng như một mô hình để đánh giá tiềm năng chống viêm hoặc phòng ngừa ung thư của các chất. Thienodolin, được phân lập từ một loài Streptomyces có nguồn gốc từ trầm tích biển của Chile, đã ức chế sản xuất nitric oxide trong các tế bào RAW 264.7 được kích thích bởi LPS (IC50 = 17,2 +/- 1,2 microM). Ở cả mức mRNA và protein, nitric oxide synthase (iNOS) có thể gây ra được ức chế theo cách phụ thuộc vào liều lượng. Các kinase hoạt hóa protein mitogen (MAPK), một con đường truyền tín hiệu chính ở thượng nguồn liên quan đến quá trình phiên mã của iNOS, không bị ảnh hưởng bởi tác dụng của thienodolin. Tuy nhiên, hợp chất này đã ngăn chặn sự thoái hóa của IkappaBa dẫn đến ức chế dịch chuyển hạt nhân NF-kappaB p65 và ức chế quá trình phosphoryl hóa các bộ chuyển đổi tín hiệu và chất hoạt hóa phiên mã 1 (STAT1) tại Tyr701. Nghiên cứu này hỗ trợ việc khám phá thêm thienodolin như một tác nhân điều trị tiềm năng với hoạt tính cơ chế độc đáo."} +{"idx": 1980060, "en": "An inventory of Sumatran medicinal plants, and reports on their chemistry and conservation carried out in the last three decades are reviewed.", "gemini": "Đã xem xét một bản kiểm kê các loại cây thuốc ở Sumatra và các báo cáo về hóa học và bảo tồn của chúng được thực hiện trong ba thập kỷ qua."} +{"idx": 1980061, "en": "It is generally accepted by all concerned that modern pharmaceuticals will remain out of reach of many people and 'health for all' may only be realized by the use of adequately assessed herbal products. Mankind has been using herbal medicine for healing right from the beginning of human civilization. With the advent of 'modern medicine' herbal products have been looked down upon, especially by western societies. Yet, in recent times, use of herbal medicine for heathcare has increased steadily all over the world. However, serious concerns are being realized regarding the safety, claimed efficacy and quality of herbal products used as herbal medicine, nutraceuticals, health food and cosmetics. Although herbal products are generally considered safe due to their age-old usage, significant side effects have been reported for many herbal products, including herbal medicine. Accidental contamination and intentional adulteration are considered as primary reasons for the side effects. The historical perspective and the philosophy of herbal medical practice along with its present status in the light of present day science have been reviewed and included in the present article. Assurance of safety by identification of contaminants and assessment of toxicity has been outlined. Assessment of claimed efficacy of herbal medicine is difficult due to its holistic approach. Practical ways of assessing efficacy of herbal medicine by adapting the methodologies used for modern pharmaceutical are described. The maintenance of standard of herbal medicine has been stressed and pragmatic approaches of assuring quality of herbal medicine by using modern tools of fingerprinting the chemical profile of herbal medicine are discussed. As much of the traditional herbal medical knowledge is scattered around the world at the family and community levels, and more so in the indigeneous people, the knowledge base is continuously being lost and so needs immediate documentation. Difficulties in documentation due to concerns of Intellectual Property Rights (IPR) have been highlighted.", "gemini": "Tất cả những người có liên quan đều chấp nhận rằng dược phẩm hiện đại sẽ vẫn nằm ngoài tầm với của nhiều người và 'sức khỏe cho mọi người' chỉ có thể đạt được bằng cách sử dụng các sản phẩm thảo dược được đánh giá đầy đủ. Loài người đã sử dụng thảo dược để chữa bệnh ngay từ đầu nền văn minh nhân loại. Với sự ra đời của 'y học hiện đại', các sản phẩm thảo dược đã bị coi thường, đặc biệt là ở các xã hội phương Tây. Tuy nhiên, trong thời gian gần đây, việc sử dụng thảo dược để chăm sóc sức khỏe đã tăng đều đặn trên toàn thế giới. Tuy nhiên, những lo ngại nghiêm trọng đang được nhận ra liên quan đến tính an toàn, hiệu quả được tuyên bố và chất lượng của các sản phẩm thảo dược được sử dụng làm thuốc thảo dược, thực phẩm chức năng, thực phẩm sức khỏe và mỹ phẩm. Mặc dù các sản phẩm thảo dược thường được coi là an toàn do được sử dụng từ lâu đời, nhưng đã có báo cáo về tác dụng phụ đáng kể đối với nhiều sản phẩm thảo dược, bao gồm cả thuốc thảo dược. Nhiễm bẩn ngẫu nhiên và cố ý làm giả được coi là nguyên nhân chính gây ra các tác dụng phụ. Quan điểm lịch sử và triết lý của thực hành y học thảo dược cùng với tình trạng hiện tại của nó dưới góc độ khoa học hiện đại đã được xem xét và đưa vào bài viết hiện tại. Đảm bảo an toàn bằng cách xác định chất gây ô nhiễm và đánh giá độc tính đã được nêu trong bài viết. Đánh giá hiệu quả được tuyên bố của thuốc thảo dược là rất khó khăn do cách tiếp cận toàn diện của nó. Các cách thực tế để đánh giá hiệu quả của thuốc thảo dược bằng cách áp dụng các phương pháp được sử dụng cho dược phẩm hiện đại được mô tả. Việc duy trì tiêu chuẩn của thuốc thảo dược đã được nhấn mạnh và các cách tiếp cận thực dụng để đảm bảo chất lượng của thuốc thảo dược bằng cách sử dụng các công cụ hiện đại để lập dấu vân tay cho hồ sơ hóa học của thuốc thảo dược được thảo luận. Vì phần lớn kiến thức y học thảo dược truyền thống nằm rải rác trên khắp thế giới ở cấp độ gia đình và cộng đồng, và nhiều hơn nữa ở người bản địa, nên cơ sở kiến thức liên tục bị mất và do đó cần được ghi chép lại ngay lập tức. Những khó khăn trong việc ghi chép do lo ngại về Quyền sở hữu trí tuệ (SHTT) đã được nêu bật."} +{"idx": 1980062, "en": "We describe the case of a patient with bilateral thalamic lesions due to brain infarcts in the paramedian thalamic artery territories. The patient demonstrated symptoms of apathy (e.g., loss of initiative and interest in others, poor motivation, flattened affect). Neuropsychological assessment 3 and 5 years post-infarct revealed severe deficits in verbal and non-verbal immediate and delayed memory, attention, and executive functioning, with minimal improvement over time. Also, he demonstrated difficulties in social cognition (i.e., perception of facial expressions of others and of sarcasm). These findings are discussed and interpreted in light of current theories regarding the neurobiological substrate of apathy.", "gemini": "Chúng tôi mô tả trường hợp của một bệnh nhân bị tổn thương thị giác hai bên do nhồi máu não ở vùng động mạch thị giác trung bình. Bệnh nhân biểu hiện các triệu chứng của chứng lãnh đạm (ví dụ: mất sáng kiến và hứng thú với người khác, động lực kém, tình cảm phẳng). Đánh giá thần kinh học 3 và 5 năm sau nhồi máu cho thấy tình trạng thiếu hụt nghiêm trọng về trí nhớ ngay lập tức và chậm trễ bằng lời nói và không bằng lời nói, sự chú ý và chức năng điều hành, với sự cải thiện tối thiểu theo thời gian. Ngoài ra, anh ta còn gặp khó khăn trong nhận thức xã hội (tức là nhận thức về biểu hiện khuôn mặt của người khác và sự mỉa mai). Những phát hiện này được thảo luận và diễn giải theo các lý thuyết hiện tại liên quan đến cơ chất thần kinh sinh học của chứng lãnh đạm."} +{"idx": 1980063, "en": "There is an increasingly acrimonious debate surrounding the use of anchored mesh in prolapse surgery. It is evident that clinicians and researchers working with this technology are under pressure from the public, from lawyers, regulators and colleagues. There is a risk that rapidly changing societal standards, championed by colleagues, lawyers and bureaucrats, will interfere with professional independence to such a degree that an entire new technology is lost before there has been time for clinical research to assess risks and benefits properly, before we learn which patients stand to benefit most, and before we get a chance to optimise implant design.", "gemini": "Có một cuộc tranh luận ngày càng gay gắt xung quanh việc sử dụng lưới neo trong phẫu thuật sa tạng. Rõ ràng là các bác sĩ lâm sàng và các nhà nghiên cứu làm việc với công nghệ này đang chịu áp lực từ công chúng, từ luật sư, cơ quan quản lý và các đồng nghiệp. Có nguy cơ các tiêu chuẩn xã hội thay đổi nhanh chóng, được các đồng nghiệp, luật sư và quan chức ủng hộ, sẽ can thiệp vào sự độc lập chuyên môn đến mức một công nghệ hoàn toàn mới bị mất trước khi có thời gian để nghiên cứu lâm sàng đánh giá đúng rủi ro và lợi ích, trước khi chúng ta biết được những bệnh nhân nào sẽ được hưởng lợi nhiều nhất và trước khi chúng ta có cơ hội tối ưu hóa thiết kế cấy ghép."} +{"idx": 1980064, "en": "In the early 2000s, the Iranian stem cell research and technology had a relatively strong start that benefited from religious blessings, political and public support, as well as scientific endeavors on the part of non-governmental and public research organizations and universities. Later on, it developed a dynamic niche market of public, private start-up, and spin-off companies and organizations that pioneered in the Islamic world in terms of ISI papers, clinical trials, and cell therapy. However, at present, it faces new challenges stemming from the insufficient finance and a comprehensive law and regulation structure to keep its momentum. To remedy this situation, the scientific community and other stakeholders need to have a series of shared long-time goals and try to build consensus on how to achieve them through nationally approved policy documents.", "gemini": "Vào đầu những năm 2000, nghiên cứu và công nghệ tế bào gốc của Iran có khởi đầu tương đối mạnh mẽ nhờ được sự ban phước của tôn giáo, sự ủng hộ của chính trị và công chúng, cũng như các nỗ lực khoa học của các tổ chức nghiên cứu phi chính phủ, công cộng và các trường đại học. Sau đó, nó phát triển một thị trường ngách năng động gồm các công ty và tổ chức khởi nghiệp tư nhân, công cộng và các công ty, tổ chức phụ trợ tiên phong trong thế giới Hồi giáo về mặt các bài báo ISI, thử nghiệm lâm sàng và liệu pháp tế bào. Tuy nhiên, hiện tại, nó phải đối mặt với những thách thức mới bắt nguồn từ việc tài chính không đủ và một cấu trúc luật pháp và quy định toàn diện để duy trì đà phát triển của nó. Để khắc phục tình trạng này, cộng đồng khoa học và các bên liên quan khác cần có một loạt các mục tiêu chung lâu dài và cố gắng xây dựng sự đồng thuận về cách đạt được các mục tiêu đó thông qua các văn kiện chính sách được chấp thuận trên toàn quốc."} +{"idx": 1980065, "en": "Evaluation of public policies with uncertain economic outcomes should consider society's preferences regarding risk. However, the preference models used in most integrated assessment models, including those commonly used to inform climate policy, do not adequately characterize the risk attitudes revealed by typical investment decisions. Here, we adopt an empirical approach to risk preference description using international historical data on investment returns and the occurrence of rare economic disasters. We improve on earlier analyses by employing a hierarchical Bayesian inference procedure that allows for nation-specific estimates of both disaster probabilities and preference parameters. This provides a stronger test of the underlying investment model than provided by previous calibrations and generates some compelling hypotheses for further study. Specifically, results suggest that society is substantially more averse to risk than typically assumed in integrated assessment models. In addition, there appear to be systematic differences in risk preferences among nations. These results are likely to have important implications for policy recommendations: higher aversion to risk increases the precautionary value of taking action to avoid low-probability, high-impact outcomes. However, geographically variable attitudes toward risk indicate that this precautionary value could vary widely across nations, thereby potentially complicating the negotiation of transboundary agreements focused on risk reduction.", "gemini": "Đánh giá các chính sách công có kết quả kinh tế không chắc chắn nên xem xét sở thích của xã hội liên quan đến rủi ro. Tuy nhiên, các mô hình sở thích được sử dụng trong hầu hết các mô hình đánh giá tích hợp, bao gồm cả các mô hình thường được sử dụng để thông báo chính sách khí hậu, không đủ đặc trưng cho thái độ rủi ro được thể hiện bởi các quyết định đầu tư thông thường. Ở đây, chúng tôi áp dụng phương pháp tiếp cận thực nghiệm để mô tả sở thích rủi ro bằng cách sử dụng dữ liệu lịch sử quốc tế về lợi nhuận đầu tư và sự xuất hiện của các thảm họa kinh tế hiếm gặp. Chúng tôi cải thiện các phân tích trước đó bằng cách sử dụng quy trình suy luận Bayes phân cấp cho phép ước tính xác suất thảm họa và các thông số sở thích cụ thể của từng quốc gia. Điều này cung cấp một bài kiểm tra mạnh mẽ hơn về mô hình đầu tư cơ bản so với các hiệu chuẩn trước đó và tạo ra một số giả thuyết hấp dẫn để nghiên cứu thêm. Cụ thể, kết quả cho thấy rằng xã hội có thái độ tránh rủi ro nhiều hơn đáng kể so với thông thường được giả định trong các mô hình đánh giá tích hợp. Ngoài ra, có vẻ như có sự khác biệt có hệ thống về sở thích rủi ro giữa các quốc gia. Những kết quả này có khả năng có ý nghĩa quan trọng đối với các khuyến nghị về chính sách: thái độ tránh rủi ro cao hơn làm tăng giá trị phòng ngừa của việc hành động để tránh những kết quả có xác suất thấp, tác động cao. Tuy nhiên, thái độ đối với rủi ro khác nhau về mặt địa lý chỉ ra rằng giá trị phòng ngừa này có thể thay đổi rất nhiều giữa các quốc gia, do đó có khả năng làm phức tạp việc đàm phán các thỏa thuận xuyên biên giới tập trung vào việc giảm rủi ro."} +{"idx": 1980066, "en": "Ligand exchange reactions are widely used for imparting new functionality on or integrating nanoparticles into devices. Thiolate-for-thiolate ligand exchange in monolayer protected gold nanoclusters has been used for over a decade; however, a firm structural basis of this reaction has been lacking. Herein, we present the first single-crystal X-ray structure of a partially exchanged Au(102)(p-MBA)(40)(p-BBT)(4) (p-MBA = para-mercaptobenzoic acid, p-BBT = para-bromobenzene thiol) with p-BBT as the incoming ligand. The crystal structure shows that 2 of the 22 symmetry-unique p-MBA ligand sites are partially exchanged to p-BBT under the initial fast kinetics in a 5 min timescale exchange reaction. Each of these ligand-binding sites is bonded to a different solvent-exposed Au atom, suggesting an associative mechanism for the initial ligand exchange. Density functional theory calculations modeling both thiol and thiolate incoming ligands postulate a mechanistic pathway for thiol-based ligand exchange. The discrete modification of a small set of ligand binding sites suggests Au(102)(p-MBA)(44) as a powerful platform for surface chemical engineering.", "gemini": "Các phản ứng trao đổi phối tử được sử dụng rộng rãi để truyền chức năng mới hoặc tích hợp các hạt nano vào các thiết bị. Trao đổi phối tử thiol-thiol trong các nano cụm vàng được bảo vệ bởi lớp đơn đã được sử dụng trong hơn một thập kỷ; tuy nhiên, vẫn thiếu một cơ sở cấu trúc vững chắc cho phản ứng này. Tại đây, chúng tôi trình bày cấu trúc tinh thể tia X đơn đầu tiên của Au(102)(p-MBA)(40)(p-BBT)(4) (p-MBA = axit para-mercaptobenzoic, p-BBT = para-bromobenzene thiol) được trao đổi một phần với p-BBT làm phối tử đến. Cấu trúc tinh thể cho thấy 2 trong số 22 vị trí phối tử p-MBA có tính đối xứng duy nhất được trao đổi một phần thành p-BBT theo động học nhanh ban đầu trong phản ứng trao đổi theo thang thời gian 5 phút. Mỗi vị trí liên kết phối tử này được liên kết với một nguyên tử Au tiếp xúc với dung môi khác nhau, cho thấy cơ chế liên hợp cho quá trình trao đổi phối tử ban đầu. Các tính toán lý thuyết hàm mật độ mô hình hóa cả phối tử thiol và thiolate đến đưa ra một con đường cơ học cho quá trình trao đổi phối tử dựa trên thiol. Việc sửa đổi riêng biệt một tập hợp nhỏ các vị trí liên kết phối tử cho thấy Au(102)(p-MBA)(44) là một nền tảng mạnh mẽ cho kỹ thuật hóa học bề mặt."} +{"idx": 1980067, "en": "Efficiency of intracoronary (IC) adenoviral vector transfection is impaired by the vascular endothelium. Ischemia and substances that increase vascular permeability (sodium nitroprusside, nitroglycerin) may augment adenoviral vector transfection efficiency (TE). We tested whether TE of adenoviral vector following IC infusion is improved by nitrates or by ischemia. Fluoroscopically guided angioplasty balloon catheters occluded the coronary artery in Yorkshire pigs and delivered adenoviral type 5 vector encoding the luciferase gene (Ad5Luc, 10(11) viral particles). TE (luciferase activity) was minimal and was not augmented by IC co-administration of 50 μg/min sodium nitroprusside to nonischemic myocardium. Two (but not one) 3-min episodes of occlusion tended to increase luciferase activity (p=0.06), and luciferase activity was further increased by IC co-administration of nitroglycerin (p<0.001). After 75 min of coronary artery occlusion, luciferase activity was greater than with shorter periods of ischemia, and was significantly greater in the ischemia-reperfused zone compared to the border zone 3 and 14 days after infusion; there was no transfection in nonischemic myocardium. IC delivery of Ad5Luc into post-ischemic myocardium caused no local inflammation or hemodynamic instability. We conclude that the uptake of IC Ad5 to ischemic reperfused myocardium validates use of IC Ad5 delivery protocols in future human gene therapy trials in patients following myocardial ischemia.", "gemini": "Hiệu quả của quá trình chuyển gen vectơ adenovirus trong động mạch vành (IC) bị suy giảm bởi nội mạc mạch máu. Tình trạng thiếu máu cục bộ và các chất làm tăng tính thấm của mạch máu (natri nitroprusside, nitroglycerin) có thể làm tăng hiệu quả chuyển gen vectơ adenovirus (TE). Chúng tôi đã kiểm tra xem TE của vectơ adenovirus sau khi truyền IC có được cải thiện bằng nitrat hay thiếu máu cục bộ không. Các ống thông bóng tạo hình mạch được hướng dẫn bằng nội soi huỳnh quang đã làm tắc động mạch vành ở lợn Yorkshire và đưa vectơ loại 5 adenovirus mã hóa gen luciferase (Ad5Luc, 10(11) hạt virus). TE (hoạt tính luciferase) là tối thiểu và không được tăng cường bởi sự đồng quản lý IC 50 μg/phút natri nitroprusside với cơ tim không thiếu máu cục bộ. Hai (nhưng không phải một) cơn tắc nghẽn trong 3 phút có xu hướng làm tăng hoạt tính luciferase (p = 0,06) và hoạt tính luciferase được tăng cường thêm bằng cách đồng quản lý IC nitroglycerin (p < 0,001). Sau 75 phút tắc động mạch vành, hoạt tính luciferase lớn hơn so với thời gian thiếu máu cục bộ ngắn hơn và lớn hơn đáng kể ở vùng tái tưới máu thiếu máu cục bộ so với vùng biên 3 và 14 ngày sau khi truyền; không có quá trình chuyển gen ở cơ tim không thiếu máu cục bộ. Việc truyền IC Ad5Luc vào cơ tim tái tưới máu sau thiếu máu cục bộ không gây ra tình trạng viêm cục bộ hoặc mất ổn định huyết động. Chúng tôi kết luận rằng sự hấp thu IC Ad5 vào cơ tim tái tưới máu thiếu máu cục bộ xác nhận việc sử dụng các giao thức truyền IC Ad5 trong các thử nghiệm liệu pháp gen ở người trong tương lai ở những bệnh nhân sau khi thiếu máu cục bộ cơ tim."} +{"idx": 1980068, "en": "Boron, a nearest-neighbor of carbon, is possibly the second element that can possess free-standing flat monolayer structures, evidenced by recent successful synthesis of single-walled and multiwalled boron nanotubes (MWBNTs). From an extensive structural search using the first-principles particle-swarm optimization (PSO) global algorithm, two boron monolayers (α(1)- and β(1)-sheet) are predicted to be the most stable α- and β-types of boron sheets, respectively. Both boron sheets possess greater cohesive energies than the state-of-the-art two-dimensional boron structures (by more than 60 meV/atom based on density functional theory calculation using PBE0 hybrid functional), that is, the α-sheet previously predicted by Tang and Ismail-Beigi and the g(1/8)- and g(2/15)-sheets (both belonging to the β-type) recently reported by Yakobson and co-workers. Moreover, the PBE0 calculation predicts that the α-sheet is a semiconductor, while the α(1)-, β(1)-, g(1/8)-, and g(2/15)-sheets are all metals. When two α(1) monolayers are stacked on top each other, the bilayer α(1)-sheet remains flat with an optimal interlayer distance of ~3.62 Å, which is close to the measured interlayer distance (~3.2 Å) in MWBNTs.", "gemini": "Bo, một nguyên tố gần nhất của cacbon, có thể là nguyên tố thứ hai có thể sở hữu các cấu trúc lớp đơn phẳng tự do, bằng chứng là sự tổng hợp thành công gần đây các ống nano bo một vách và nhiều vách (MWBNT). Từ một quá trình tìm kiếm cấu trúc mở rộng bằng thuật toán toàn cục tối ưu hóa đàn hạt (PSO) nguyên lý đầu tiên, hai lớp đơn bo (tấm α(1)- và β(1)) được dự đoán là các loại tấm bo α- và β-ổn định nhất, tương ứng. Cả hai tấm bo đều sở hữu năng lượng gắn kết lớn hơn các cấu trúc bo hai chiều hiện đại (hơn 60 meV/nguyên tử dựa trên tính toán lý thuyết hàm mật độ sử dụng hàm số lai PBE0), tức là tấm α trước đây được Tang và Ismail-Beigi dự đoán và tấm g(1/8)- và g(2/15)- (cả hai đều thuộc loại β) gần đây được Yakobson và cộng sự báo cáo. Hơn nữa, phép tính PBE0 dự đoán rằng tấm α là chất bán dẫn, trong khi các tấm α(1)-, β(1)-, g(1/8)- và g(2/15)- đều là kim loại. Khi hai lớp đơn α(1) được xếp chồng lên nhau, lớp đơn α(1) hai lớp vẫn phẳng với khoảng cách giữa các lớp tối ưu là ~3,62 Å, gần với khoảng cách giữa các lớp được đo (~3,2 Å) trong MWBNT."} +{"idx": 1980069, "en": "AIMS The aim of our research was to isolate the compounds from the metabolites of Raoultella ornithinolytica with the activity against Candida albicans and to analyse the action of the compounds on the metabolic activity and morphology of the fungus cells. METHODS AND RESULTS The effect of active protein fractions on the cell morphology, growth, and metabolic activity of C. albicans was analysed under a light microscope with Nomarski contrast and after staining with calcofluor-white. The LIVE/DEAD Yeast Viability Kit F-7030 FUN 1 was used for determination of C. albicans metabolic activity. The biomolecules obtained after isolation by ion exchange chromatography were further fractionated by Sephadex G-50 medium gel filtration. Then, after molecular sieve, the fractions were analysed by FTIR and SERS Spectroscopy. A subfraction was isolated from the antifungal protein fraction above 100 kDa. The active subfraction identified as the glyco-protein complex caused a decrease in the metabolic activity and morphological changes of C. albicans cells. CONCLUSIONS The glyco-protein complex obtained from metabolites of bacteria Raoultella ornithinolytica possesses antifungal activity against C. albicans and shows minimal toxicity (1%) against fibroblasts. SIGNIFICANCE AND IMPACT OF THE STUDY Studies on the glyco-protein complex obtained from earthworm gut bacteria R. ornithinolytica can lead to their application in biological fungicide and pharmaceutical industry.", "gemini": "MỤC TIÊU Mục tiêu nghiên cứu của chúng tôi là phân lập các hợp chất từ các chất chuyển hóa của Raoultella ornithinolytica có hoạt tính chống lại Candida albicans và phân tích tác dụng của các hợp chất đối với hoạt động trao đổi chất và hình thái của các tế bào nấm. PHƯƠNG PHÁP VÀ KẾT QUẢ Tác dụng của các phân đoạn protein hoạt động đối với hình thái tế bào, sự phát triển và hoạt động trao đổi chất của C. albicans đã được phân tích dưới kính hiển vi quang học với độ tương phản Nomarski và sau khi nhuộm bằng calcofluor-trắng. Bộ dụng cụ xác định khả năng sống của nấm men LIVE/DEAD F-7030 FUN 1 được sử dụng để xác định hoạt động trao đổi chất của C. albicans. Các phân tử sinh học thu được sau khi phân lập bằng sắc ký trao đổi ion được tiếp tục phân đoạn bằng phương pháp lọc gel Sephadex G-50. Sau đó, sau khi lọc sàng phân tử, các phân đoạn được phân tích bằng quang phổ FTIR và SERS. Một phân đoạn nhỏ đã được phân lập từ phân đoạn protein kháng nấm có kích thước trên 100 kDa. Phân đoạn nhỏ hoạt động được xác định là phức hợp glyco-protein gây ra sự giảm hoạt động trao đổi chất và những thay đổi về hình thái của các tế bào C. albicans. KẾT LUẬN Phức hợp glyco-protein thu được từ các chất chuyển hóa của vi khuẩn Raoultella ornithinolytica có hoạt tính kháng nấm đối với C. albicans và thể hiện độc tính tối thiểu (1%) đối với nguyên bào sợi. Ý NGHĨA VÀ TÁC ĐỘNG CỦA NGHIÊN CỨU Các nghiên cứu về phức hợp glyco-protein thu được từ vi khuẩn đường ruột giun đất R. ornithinolytica có thể dẫn đến ứng dụng của chúng trong ngành công nghiệp thuốc trừ nấm sinh học và dược phẩm."} +{"idx": 1980070, "en": "Evolution proceeds unceasingly in all biological populations. It is clear that climate-driven evolution has molded plants in deep time and within extant populations. However, it is less certain whether adaptive evolution can proceed sufficiently rapidly to maintain the fitness and demographic stability of populations subjected to exceptionally rapid contemporary climate change. Here, we consider this question, drawing on current evidence on the rate of plant range shifts and the potential for an adaptive evolutionary response. We emphasize advances in understanding based on theoretical studies that model interacting evolutionary processes, and we provide an overview of quantitative genetic approaches that can parameterize these models to provide more meaningful predictions of the dynamic interplay between genetics, demography and evolution. We outline further research that can clarify both the adaptive potential of plant populations as climate continues to change and the role played by ongoing adaptation in their persistence.", "gemini": "Sự tiến hóa diễn ra không ngừng trong tất cả các quần thể sinh học. Rõ ràng là sự tiến hóa do khí hậu thúc đẩy đã định hình thực vật trong thời gian dài và trong các quần thể hiện có. Tuy nhiên, vẫn chưa chắc chắn liệu sự tiến hóa thích nghi có thể diễn ra đủ nhanh để duy trì sự phù hợp và sự ổn định về mặt nhân khẩu học của các quần thể phải chịu sự thay đổi khí hậu đương đại cực kỳ nhanh chóng hay không. Ở đây, chúng tôi xem xét câu hỏi này, dựa trên các bằng chứng hiện tại về tốc độ thay đổi phạm vi thực vật và tiềm năng của phản ứng tiến hóa thích nghi. Chúng tôi nhấn mạnh những tiến bộ trong việc hiểu biết dựa trên các nghiên cứu lý thuyết mô hình hóa các quá trình tiến hóa tương tác và chúng tôi cung cấp một cái nhìn tổng quan về các phương pháp di truyền định lượng có thể tham số hóa các mô hình này để đưa ra những dự đoán có ý nghĩa hơn về sự tương tác động giữa di truyền học, nhân khẩu học và tiến hóa. Chúng tôi phác thảo thêm các nghiên cứu có thể làm rõ cả tiềm năng thích nghi của các quần thể thực vật khi khí hậu tiếp tục thay đổi và vai trò của sự thích nghi đang diễn ra trong sự tồn tại của chúng."} +{"idx": 1980071, "en": "With increasing age, people experience more difficulties with suppressing irrelevant information, which may have a major impact on cognitive functioning. The extent of decline of inhibitory functions with age is highly variable between individuals. In this study, we used ERPs and phase locking analyses to investigate neural correlates of this variability in inhibition between individuals. Older and younger participants performed a selective attention task in which relevant and irrelevant information was presented simultaneously. The participants were split into high and low performers based on their level of inhibition inefficiency, that is, the slowing of RTs induced by information that participants were instructed to ignore. P1 peak amplitudes were larger in low performers than in high performers, indicating that low performers were less able to suppress the processing of irrelevant stimuli. Phase locking analyses were used as a measure of functional connectivity. Efficient inhibition in both age groups was related to the increased functional connectivity in the alpha band between frontal and occipito-parietal ROIs in the prestimulus interval. In addition, increased power in the alpha band in occipito-parietal ROIs was related to better inhibition both before and after stimulus onset. Phase locking in the upper beta band before and during stimulus presentation between frontal and occipito-parietal ROIs was related to a better performance in older participants only, suggesting that this is an active compensation mechanism employed to maintain adequate performance. In addition, increased top-down modulation and increased power in the alpha band appears to be a general mechanism facilitating inhibition in both age groups.", "gemini": "Càng lớn tuổi, mọi người càng gặp nhiều khó khăn trong việc ngăn chặn thông tin không liên quan, điều này có thể ảnh hưởng lớn đến chức năng nhận thức. Mức độ suy giảm chức năng ức chế theo tuổi tác rất khác nhau giữa các cá nhân. Trong nghiên cứu này, chúng tôi đã sử dụng ERP và phân tích khóa pha để điều tra mối tương quan về thần kinh của sự thay đổi này trong quá trình ức chế giữa các cá nhân. Những người tham gia lớn tuổi và trẻ tuổi đã thực hiện một nhiệm vụ chú ý có chọn lọc trong đó thông tin có liên quan và không liên quan được trình bày đồng thời. Những người tham gia được chia thành những người có hiệu suất cao và thấp dựa trên mức độ ức chế kém hiệu quả của họ, tức là làm chậm thời gian phản ứng do thông tin mà người tham gia được hướng dẫn bỏ qua. Biên độ đỉnh P1 lớn hơn ở những người có hiệu suất thấp so với những người có hiệu suất cao, cho thấy những người có hiệu suất thấp kém hơn trong việc ngăn chặn quá trình xử lý các kích thích không liên quan. Phân tích khóa pha được sử dụng như một thước đo kết nối chức năng. Sự ức chế hiệu quả ở cả hai nhóm tuổi có liên quan đến sự gia tăng kết nối chức năng trong băng tần alpha giữa các vùng quan tâm trước trán và chẩm đỉnh trong khoảng thời gian trước kích thích. Ngoài ra, công suất tăng lên trong băng tần alpha ở các vùng quan tâm chẩm đỉnh có liên quan đến sự ức chế tốt hơn cả trước và sau khi kích thích bắt đầu. Khóa pha trong băng tần beta trên trước và trong khi trình bày kích thích giữa các vùng quan tâm trước trán và chẩm đỉnh chỉ liên quan đến hiệu suất tốt hơn ở những người tham gia lớn tuổi, điều này cho thấy đây là một cơ chế bù đắp tích cực được sử dụng để duy trì hiệu suất đầy đủ. Ngoài ra, tăng điều chế từ trên xuống và tăng công suất trong băng tần alpha dường như là một cơ chế chung tạo điều kiện ức chế ở cả hai nhóm tuổi."} +{"idx": 1980072, "en": "Failed knowledge recall attempts are sometimes accompanied by a strong feeling of imminent success, giving rise to a \"tip-of-the-tongue\" (TOT) experience. Similar to successful retrieval (i.e., the Know state, K), a TOT commences with strong cue familiarity but involves only partial retrieval of related information. We sought to characterize the cognitive processes and temporal dynamics of these retrieval states and to extend the applicability of previous findings about TOT to the auditory modality. Participants heard 3-sec initial segments of popular songs and were asked to recall their names. EEG was recorded while participants indicated their retrieval state via button press. Stimulus-locked analyses revealed a significant early left fronto-central difference between TOT and K, at 300-550 msec postcue onset. Post hoc analysis revealed that, in this time window, TOT also differed from DK (Don't Know) responses, which themselves were similar to the K responses. This finding indicates that neural processes, which may reflect strategy selection, ease of semantic processing, familiarity-related processes, or conflict monitoring, are indicative of the fate of our knowledge judgments long before we actually execute them.", "gemini": "Những nỗ lực hồi tưởng kiến thức không thành công đôi khi đi kèm với cảm giác thành công sắp xảy ra, dẫn đến trải nghiệm \"đến tận cùng lưỡi\" (TOT). Tương tự như việc truy xuất thành công (tức là trạng thái Biết, K), TOT bắt đầu với sự quen thuộc với tín hiệu mạnh mẽ nhưng chỉ liên quan đến việc truy xuất một phần thông tin liên quan. Chúng tôi tìm cách mô tả các quá trình nhận thức và động lực thời gian của các trạng thái truy xuất này và mở rộng khả năng áp dụng các phát hiện trước đây về TOT vào phương thức thính giác. Những người tham gia đã nghe các đoạn đầu dài 3 giây của các bài hát phổ biến và được yêu cầu nhớ lại tên bài hát. Điện não đồ được ghi lại trong khi những người tham gia chỉ ra trạng thái truy xuất của họ thông qua nút bấm. Các phân tích khóa kích thích cho thấy sự khác biệt rõ rệt giữa TOT và K ở vùng trán trái phía trước, trong khoảng 300-550 ms sau khi bắt đầu tín hiệu. Phân tích sau thử nghiệm cho thấy, trong cửa sổ thời gian này, TOT cũng khác với các phản ứng DK (Không biết), bản thân các phản ứng này tương tự như phản ứng K. Phát hiện này chỉ ra rằng các quá trình thần kinh, có thể phản ánh sự lựa chọn chiến lược, dễ xử lý ngữ nghĩa, các quá trình liên quan đến sự quen thuộc hoặc theo dõi xung đột, biểu thị số phận của các phán đoán kiến thức của chúng ta rất lâu trước khi chúng ta thực sự thực hiện chúng."} +{"idx": 1980073, "en": "The therapeutic efficiency of mangiferin is restricted by its low intestinal permeability. In order to improve the oral absorption of mangiferin, potential of enhancers, including TPGS, sodium deoxycholate and Carbopol 974P, were investigated in a series of in vivo experiments. After administration of mangiferin at a dose of 30 mg/kg combining with sodium deoxycholate, the bioavailability of mangiferin increased four-fold, and this may be due to sodium deoxycholate weakening the compactness between lecithin molecules and increased the paracellular permeability. When Carbopol 974P (100 mg/kg) was combined with mangiferin, the oral bioavailability of it increased seven-fold compared with the control group, and this may be related to the mucoadhesive properties of Carbopol 974P and paracellular drug permeation. However, following coadministration of TPGS (50 mg/kg), the oral absorption of mangiferin increased slightly compared with that of the control group (p > 0.05). The increased oral absorption by the three coexcipients was in the order of Carbopol 974P > sodium deoxycholate > TPGS. The absolute bioavailability of mangiferin in the three different doses following oral administration were evaluated based on the AUC(0-t) of the intravenous dose and there was no increase from low doses to high doses (25 mg/kg to 100 mg/kg). The above results show that the low absorption of mangiferin was due to presence of a narrow absorption window, which may also exist in these compounds, which have structures similar to mangiferin including three fused aromatic rings with polyphenolic hydroxyl groups. Bioadhesion polymers are effective enhancers of the absorption of mangiferin in the gastrointestinal tract.", "gemini": "Hiệu quả điều trị của mangiferin bị hạn chế bởi tính thấm ruột thấp. Để cải thiện khả năng hấp thụ qua đường uống của mangiferin, tiềm năng của các chất tăng cường, bao gồm TPGS, natri deoxycholate và Carbopol 974P, đã được nghiên cứu trong một loạt các thí nghiệm in vivo. Sau khi dùng mangiferin với liều 30 mg/kg kết hợp với natri deoxycholate, khả dụng sinh học của mangiferin tăng gấp bốn lần, và điều này có thể là do natri deoxycholate làm suy yếu sự chặt chẽ giữa các phân tử lecithin và tăng tính thấm của tế bào. Khi kết hợp Carbopol 974P (100 mg/kg) với mangiferin, khả dụng sinh học qua đường uống của nó tăng gấp bảy lần so với nhóm đối chứng, và điều này có thể liên quan đến các đặc tính kết dính niêm mạc của Carbopol 974P và sự thẩm thấu thuốc qua tế bào. Tuy nhiên, sau khi dùng đồng thời TPGS (50 mg/kg), khả năng hấp thụ qua đường uống của mangiferin tăng nhẹ so với nhóm đối chứng (p > 0,05). Sự gia tăng hấp thụ qua đường uống bởi ba tá dược là theo thứ tự Carbopol 974P > natri deoxycholate > TPGS. Khả dụng sinh học tuyệt đối của mangiferin ở ba liều khác nhau sau khi dùng đường uống được đánh giá dựa trên AUC(0-t) của liều tiêm tĩnh mạch và không có sự gia tăng từ liều thấp đến liều cao (25 mg/kg đến 100 mg/kg). Các kết quả trên cho thấy sự hấp thụ thấp của mangiferin là do sự hiện diện của cửa sổ hấp thụ hẹp, cũng có thể tồn tại ở những hợp chất này, có cấu trúc tương tự như mangiferin bao gồm ba vòng thơm hợp nhất với nhóm hydroxyl polyphenol. Các polyme kết dính sinh học là chất tăng cường hiệu quả hấp thụ mangiferin trong đường tiêu hóa."} +{"idx": 1980074, "en": "BACKGROUND The different settings of the automatic algorithm in the Carto system (Carto XP, Biosense Webster, Diamond Bar, CA, USA) used for detecting complex fractionated electrograms (CFEs) during atrial fibrillation (AF) may influence the identification of the fragmented electrograms. OBJECTIVES We aimed to evaluate the impact of the different parameters on the detection of CFEs and the efficacy of the substrate modification after pulmonary vein isolation (PVI). METHODS A total of 1,159 electrograms were analyzed from 11 consecutive patients (age = 56 ± 12 years). The effect of the different algorithm factors, such as the high-voltage thresholds (0.12, 0.25, 0.5, 20 mV), detection algorithms (average complex interval [ACI] vs interval confidence level), and recording duration (2.5 seconds vs 5 seconds), on the disparities of the CFEs was investigated. RESULTS The proportion of the different grades of CFEs depended on the detection algorithm and recording duration. The high-voltage threshold would not affect the consistency of the CFEs irrespective of the different settings of the detection algorithm or recording duration. High-grade CFEs were most consistent with an ACI algorithm and recording duration of 5 seconds (Cronbach's alpha = 0.952). Ablation consisting of a PVI and high-grade CFE sites converted AF directly to sinus rhythm in eight of 11 patients or into atrial tachycardia in one of 11. CONCLUSIONS The distribution and consistency of the CFE detection depended on the detection algorithm and recording duration, but not on the high-voltage threshold. Under the ACI algorithm and a recording duration of 5 seconds, high-grade CFE sites remained highest consistency.", "gemini": "HOÀN CẢNH Các cài đặt khác nhau của thuật toán tự động trong hệ thống Carto (Carto XP, Biosense Webster, Diamond Bar, CA, Hoa Kỳ) được sử dụng để phát hiện các điện đồ phân đoạn phức tạp (CFE) trong rung nhĩ (AF) có thể ảnh hưởng đến việc xác định các điện đồ phân đoạn. MỤC TIÊU Chúng tôi nhằm mục đích đánh giá tác động của các thông số khác nhau đối với việc phát hiện CFE và hiệu quả của việc sửa đổi cơ chất sau khi cô lập tĩnh mạch phổi (PVI). PHƯƠNG PHÁP Tổng cộng 1.159 điện đồ đã được phân tích từ 11 bệnh nhân liên tiếp (tuổi = 56 ± 12 năm). Tác động của các yếu tố thuật toán khác nhau, chẳng hạn như ngưỡng điện áp cao (0,12, 0,25, 0,5, 20 mV), thuật toán phát hiện (khoảng tin cậy trung bình [ACI] so với mức độ tin cậy khoảng) và thời gian ghi (2,5 giây so với 5 giây), đối với sự chênh lệch của CFE đã được nghiên cứu. KẾT QUẢ Tỷ lệ các mức độ CFE khác nhau phụ thuộc vào thuật toán phát hiện và thời gian ghi. Ngưỡng điện áp cao sẽ không ảnh hưởng đến tính nhất quán của CFE bất kể các cài đặt khác nhau của thuật toán phát hiện hoặc thời gian ghi. CFE cấp cao nhất phù hợp nhất với thuật toán ACI và thời gian ghi là 5 giây (alpha Cronbach = 0,952). Phá hủy bao gồm PVI và các vị trí CFE cấp cao đã chuyển AF trực tiếp thành nhịp xoang ở tám trong số 11 bệnh nhân hoặc thành nhịp nhanh nhĩ ở một trong số 11 bệnh nhân. KẾT LUẬN Sự phân bố và tính nhất quán của phát hiện CFE phụ thuộc vào thuật toán phát hiện và thời gian ghi, nhưng không phụ thuộc vào ngưỡng điện áp cao. Theo thuật toán ACI và thời gian ghi là 5 giây, các vị trí CFE cấp cao vẫn có độ nhất quán cao nhất."} +{"idx": 1980075, "en": "AIMS The study aims to determine the clinical outcomes of aripiprazole treatment in adolescents and young adults with schizophrenia spectrum disorders. METHODS This was a 24-week, observational, prospective study. Patients with schizophrenia spectrum disorders were prescribed a daily dose of 5 mg to 30 mg of aripiprazole. Effectiveness was assessed by the change from baseline in psychotic symptoms and quality of life. RESULTS Forty-two patients with a mean age of 18.1 ± 3.7 years were recruited. Eighteen were experiencing the first episode of psychosis (FEP), whereas the remaining 24 were non-FEP. Psychotic symptoms, but not quality of life, improved globally from baseline scores by the endpoint of the study (effect size = 0.44). Compared with non-FEP patients, FEP patients had greater improvements (effect size = 0.45) in some clinical outcome dimensions during the 24-week aripiprazole treatment. CONCLUSION We observed significant improvements of medium effect sizes in psychotic symptoms of patients with schizophrenia spectrum disorders within a naturalistic clinical setting, especially for FEP patients.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm xác định kết quả lâm sàng của việc điều trị bằng aripiprazole ở thanh thiếu niên và người trẻ tuổi mắc các rối loạn phổ tâm thần phân liệt. PHƯƠNG PHÁP Đây là một nghiên cứu quan sát, có triển vọng, kéo dài 24 tuần. Bệnh nhân mắc các rối loạn phổ tâm thần phân liệt được kê đơn liều hàng ngày từ 5 mg đến 30 mg aripiprazole. Hiệu quả được đánh giá bằng sự thay đổi từ mức cơ bản về các triệu chứng loạn thần và chất lượng cuộc sống. KẾT QUẢ Đã tuyển dụng bốn mươi hai bệnh nhân có độ tuổi trung bình là 18,1 ± 3,7. Mười tám người đang trải qua cơn loạn thần đầu tiên (FEP), trong khi 24 người còn lại không phải FEP. Các triệu chứng loạn thần, nhưng không phải chất lượng cuộc sống, đã được cải thiện toàn cầu từ các điểm cơ bản vào thời điểm kết thúc nghiên cứu (kích thước hiệu ứng = 0,44). So với những bệnh nhân không mắc FEP, những bệnh nhân mắc FEP có sự cải thiện lớn hơn (kích thước hiệu ứng = 0,45) ở một số chiều kích kết quả lâm sàng trong quá trình điều trị bằng aripiprazole trong 24 tuần. KẾT LUẬN Chúng tôi đã quan sát thấy sự cải thiện đáng kể về kích thước hiệu ứng trung bình đối với các triệu chứng loạn thần ở những bệnh nhân mắc các rối loạn phổ tâm thần phân liệt trong bối cảnh lâm sàng tự nhiên, đặc biệt là đối với bệnh nhân FEP."} +{"idx": 1980076, "en": "AIM Increasing numbers of older people are admitted to hospital as medical emergencies. They are a heterogeneous population with uncertain trajectories and outcomes. Our aim was to retrospectively characterize subgroups of older inpatients based on their acuity trajectories. METHODS This was a single-center patient series from St James's Hospital Dublin, Ireland (2002-2010). The Medical Admissions Risk System (MARS) score was used to classify a sample of 14,607 patients aged ≥ 65 years, from admission to end of episode, into four trajectory groups: (i) static high acuity (group 1); (ii) static low acuity (group 2); (iii) inpatient deterioration (group 3); and (iv) inpatient improvement (group 4). K-means cluster analysis was used for the classification. RESULTS Group 1 (4.1%): median length of stay (LOS) 7.4 days, 23.6% used intensive care, mortality rate 79.2%; sepsis and renal failure were the dominant features. Group 2 (76.6%): median LOS 8.0 days, 5.2% used intensive care, mortality rate 9.5%; younger age, low comorbidity and diseases of non-vital organs were predominant. Group 3 (7.6%): median LOS 17.2 days, 17.4% used intensive care, mortality rate 76.1%; high comorbidity and sepsis/respiratory disease featured. Group 4 (11.7%): median LOS 12.1 days, 12.8% used intensive care, mortality rate 22.7%; sepsis and renal/metabolic disease were frequent, and comorbidity levels were intermediate. CONCLUSIONS In older acute medical inpatients, the outcome seemed more driven by specific diagnoses (such as sepsis and renal failure) and comorbidity, than by age. Using the MARS score to retrospectively categorize older inpatients might help to understand their heterogeneity and promote the design of appropriate care pathways.", "gemini": "MỤC ĐÍCH Ngày càng có nhiều người cao tuổi được đưa vào bệnh viện vì các trường hợp cấp cứu y tế. Họ là một nhóm dân số không đồng nhất với các diễn biến và kết cục không chắc chắn. Mục đích của chúng tôi là mô tả lại các nhóm bệnh nhân nội trú cao tuổi dựa trên các diễn biến cấp tính của họ. PHƯƠNG PHÁP Đây là một loạt bệnh nhân tại một trung tâm từ Bệnh viện St James's Dublin, Ireland (2002-2010). Điểm số Hệ thống rủi ro nhập viện y khoa (MARS) được sử dụng để phân loại một mẫu gồm 14.607 bệnh nhân từ 65 tuổi trở lên, từ khi nhập viện đến cuối đợt, thành bốn nhóm diễn biến: (i) cấp tính cao tĩnh (nhóm 1); (ii) cấp tính thấp tĩnh (nhóm 2); (iii) suy giảm bệnh nhân nội trú (nhóm 3); và (iv) cải thiện bệnh nhân nội trú (nhóm 4). Phân tích cụm K-means được sử dụng để phân loại. KẾT QUẢ Nhóm 1 (4,1%): thời gian nằm viện trung bình (LOS) 7,4 ngày, 23,6% sử dụng chăm sóc đặc biệt, tỷ lệ tử vong 79,2%; nhiễm trùng huyết và suy thận là những đặc điểm nổi bật. Nhóm 2 (76,6%): LOS trung bình 8,0 ngày, 5,2% sử dụng chăm sóc đặc biệt, tỷ lệ tử vong 9,5%; tuổi trẻ hơn, bệnh đi kèm ít và các bệnh không ảnh hưởng đến các cơ quan quan trọng là chủ yếu. Nhóm 3 (7,6%): LOS trung bình 17,2 ngày, 17,4% sử dụng chăm sóc đặc biệt, tỷ lệ tử vong 76,1%; bệnh đi kèm nhiều và nhiễm trùng huyết/bệnh đường hô hấp có đặc điểm. Nhóm 4 (11,7%): LOS trung bình 12,1 ngày, 12,8% sử dụng chăm sóc đặc biệt, tỷ lệ tử vong 22,7%; nhiễm trùng huyết và bệnh thận/chuyển hóa thường gặp và mức độ bệnh đi kèm ở mức trung bình. KẾT LUẬN Ở những bệnh nhân nội trú cấp tính cao tuổi, kết cục có vẻ bị chi phối nhiều hơn bởi các chẩn đoán cụ thể (như nhiễm trùng huyết và suy thận) và bệnh đi kèm, hơn là do tuổi tác. Việc sử dụng điểm MARS để phân loại lại các bệnh nhân nội trú cao tuổi có thể giúp hiểu được tính không đồng nhất của họ và thúc đẩy việc thiết kế các lộ trình chăm sóc phù hợp."} +{"idx": 1980077, "en": "INTRODUCTION Diabetes is a condition that requires adequate care to ensure ideal outcomes. One need is for proper post-discharge follow-up care to reduce unnecessary hospital re-admissions. This care is more difficult in US rural areas due to lower physician and resource availability. The purpose of this analysis was to examine US urban-rural differences in 30 day post-discharge physician follow-up care. METHODS This analysis utilized data from 2005 Medicare claims files, merged with county-level information from the area resource file. Beneficiaries with diabetes and with a hospitalization served as the study population. Differences in hospitalization rates and 30 day physician follow-up care were estimated across levels of rurality. Multi-level multivariate models estimated the factors that significantly contributed to obtaining such care. RESULTS Approximately 90% of the study population had a follow-up physician visit within 30 days; this rate was lower among rural beneficiaries. Adjusted estimates indicated that beneficiaries in rural areas were not less likely to obtain a follow-up visit. Factors associated with obtaining a follow up included having addition comorbidities, being female or White, and living in the US Northeast. CONCLUSIONS This analysis found evidence that rural Medicare beneficiaries were less likely to obtain post-discharge physician follow-up visits within 30 days. The adjusted result indicate that other factors such as personal demographic and illness characteristics are more predictive of this behavior than the rural location itself. More research is needed to identify why these specific factors are associated with visit behavior, and how to design interventions to improve these rates.", "gemini": "GIỚI THIỆU Bệnh tiểu đường là một tình trạng cần được chăm sóc đầy đủ để đảm bảo kết quả lý tưởng. Một nhu cầu là chăm sóc theo dõi sau khi xuất viện thích hợp để giảm tình trạng tái nhập viện không cần thiết. Việc chăm sóc này khó khăn hơn ở các vùng nông thôn của Hoa Kỳ do tình trạng thiếu bác sĩ và nguồn lực. Mục đích của phân tích này là kiểm tra sự khác biệt giữa thành thị và nông thôn của Hoa Kỳ trong việc theo dõi bác sĩ sau khi xuất viện 30 ngày. PHƯƠNG PHÁP Phân tích này sử dụng dữ liệu từ các tệp yêu cầu bồi thường của Medicare năm 2005, được kết hợp với thông tin cấp quận từ tệp tài nguyên khu vực. Những người hưởng lợi mắc bệnh tiểu đường và phải nhập viện được coi là quần thể nghiên cứu. Sự khác biệt về tỷ lệ nhập viện và việc theo dõi bác sĩ sau 30 ngày được ước tính trên các cấp độ nông thôn. Các mô hình đa biến đa cấp ước tính các yếu tố đóng góp đáng kể vào việc có được sự chăm sóc như vậy. KẾT QUẢ Khoảng 90% dân số nghiên cứu đã có một cuộc khám bác sĩ theo dõi trong vòng 30 ngày; tỷ lệ này thấp hơn ở những người thụ hưởng ở nông thôn. Các ước tính điều chỉnh chỉ ra rằng những người thụ hưởng ở các vùng nông thôn không có khả năng khám theo dõi ít hơn. Các yếu tố liên quan đến việc khám theo dõi bao gồm có thêm bệnh đi kèm, là nữ hoặc da trắng và sống ở vùng Đông Bắc Hoa Kỳ. KẾT LUẬN Phân tích này tìm thấy bằng chứng cho thấy những người hưởng lợi từ Medicare ở nông thôn ít có khả năng khám theo dõi của bác sĩ sau khi xuất viện trong vòng 30 ngày. Kết quả điều chỉnh chỉ ra rằng các yếu tố khác như đặc điểm nhân khẩu học cá nhân và bệnh tật có khả năng dự đoán hành vi này hơn là bản thân địa điểm ở nông thôn. Cần nhiều nghiên cứu hơn để xác định lý do tại sao các yếu tố cụ thể này có liên quan đến hành vi khám và cách thiết kế các biện pháp can thiệp để cải thiện các tỷ lệ này."} +{"idx": 1980078, "en": "AIM The aim of the study was to describe seizure outcome following surgery for focal extratemporal epilepsy and identify factors associated with prolonged postsurgical freedom from seizures. METHOD In this retrospective cohort study, children with drug-resistant focal extratemporal epilepsy were treated surgically and followed up in a single tertiary care centre between 1997 and 2008. RESULTS Eighty children were identified for inclusion in the study (42 males, 38 females; median age 9y 1mo, range 3mo-18y 7mo). The aetiology was identified as focal cortical dysplasia (n=37), low-grade tumour (n=22), tuberous sclerosis (n=9), or non-specific (n=12). Children were followed for a median of 3 years 1 month (range 8mo-10y 7mo) after surgery. Overall, at last follow-up, 50% of the children had been completely seizure free since surgery (Engel class Ia); of these 40 individuals, 15 had discontinued all antiepileptic drugs. Several presurgical factors were associated with a favourable outcome. However, after controlling for confounding factors, aetiology appeared to be the only determinant of long-term seizure outcome as non-specific lesion pathology was associated with seizure recurrence (hazard ratio 10.43; 95% confidence interval 3.26-33.39). INTERPRETATION In 50% of cases, children with surgically treated drug-resistant extratemporal epilepsies have an excellent long-term outcome. The aetiology of the epileptogenic lesion appears to be the only significant determinant of surgical outcome in this population of children. It is difficult to correctly identify non-specific pathology on presurgical magnetic resonance imaging.", "gemini": "MỤC TIÊU Mục tiêu của nghiên cứu này là mô tả kết cục cơn động kinh sau phẫu thuật động kinh ngoại thái dương khu trú và xác định các yếu tố liên quan đến việc không lên cơn động kinh kéo dài sau phẫu thuật. PHƯƠNG PHÁP Trong nghiên cứu đoàn hệ hồi cứu này, trẻ em mắc chứng động kinh ngoại thái dương khu trú kháng thuốc đã được phẫu thuật và theo dõi tại một trung tâm chăm sóc sức khỏe chuyên khoa trong giai đoạn từ năm 1997 đến năm 2008. KẾT QUẢ Có tám mươi trẻ em được xác định đủ điều kiện tham gia nghiên cứu (42 trẻ nam, 38 trẻ nữ; độ tuổi trung bình là 9 tuổi 1 tháng, dao động từ 3 tháng đến 18 tuổi 7 tháng). Nguyên nhân được xác định là loạn sản vỏ não khu trú (n = 37), khối u cấp độ thấp (n = 22), xơ củ (n = 9) hoặc không đặc hiệu (n = 12). Trẻ em được theo dõi trong thời gian trung bình là 3 năm 1 tháng (dao động từ 8 tháng đến 10 năm 7 tháng) sau phẫu thuật. Nhìn chung, tại lần theo dõi cuối cùng, 50% trẻ em đã không lên cơn động kinh kể từ sau phẫu thuật (lớp Engel Ia); trong số 40 cá nhân này, 15 cá nhân đã ngừng sử dụng tất cả các loại thuốc chống động kinh. Một số yếu tố trước phẫu thuật có liên quan đến kết cục thuận lợi. Tuy nhiên, sau khi kiểm soát các yếu tố gây nhiễu, nguyên nhân dường như là yếu tố duy nhất quyết định kết cục cơn động kinh dài hạn vì bệnh lý tổn thương không đặc hiệu có liên quan đến tái phát cơn động kinh (tỷ lệ nguy cơ 10,43; khoảng tin cậy 95% 3,26-33,39). GIẢI THÍCH Trong 50% trường hợp, trẻ em được phẫu thuật điều trị chứng động kinh ngoại thái dương kháng thuốc có kết cục dài hạn tuyệt vời. Nguyên nhân gây ra tổn thương gây động kinh dường như là yếu tố quyết định duy nhất có ý nghĩa đối với kết cục phẫu thuật ở nhóm trẻ em này. Thật khó để xác định chính xác bệnh lý không đặc hiệu trên hình ảnh cộng hưởng từ trước phẫu thuật."} +{"idx": 1980079, "en": "BACKGROUND AND OBJECTIVE Idiopathic pulmonary fibrosis (IPF) is the commonest idiopathic interstitial pneumonia and carries a poor prognosis. Epidemiological evidence suggests that patients with IPF have an increased risk of developing lung cancer. Carcinoembryonic antigen (CEA) has a close association with epithelial malignancy. The aim of this study was to evaluate serum CEA concentrations in patients with IPF and to perform correlation with pulmonary function. METHODS Serum CEA concentrations were measured by two-site sequential chemiluminescent immunometric assay in 41 non-smoking patients with IPF. Patients with a history of gastrointestinal tract malignancy or other disorder known to be associated with raised serum CEA were excluded. RESULTS A total of 41 patients were evaluated. The mean (±standard deviation) age of patients was 73 ± 7 years. The mean (±standard deviation) forced vital capacity was 88 ± 20% of predicted, and the mean (±standard deviation) diffusing factor for carbon monoxide (DLco) was 52 ± 19% of predicted. Twenty-one (51%) patients had a serum CEA concentration higher than upper limit of the normal range (0-5 ng/mL). CEA concentration was significantly negatively correlated with lung function (P = 0.005; R(2) = 0.20 for forced vital capacity and P = 0.006; R(2) = 0.20 for DLco). Raised CEA level also correlated significantly with the extent of fibrosis. A lung biopsy specimen from a patient with IPF demonstrated strong staining for CEA in metaplastic epithelium lining the honeycombed cysts and respiratory bronchioles. CONCLUSIONS Serum CEA concentration is elevated in approximately half of patients with IPF and is correlated with disease severity. Immunohistochemical staining reveals that CEA localizes to metaplastic epithelium lining honeycombed bronchioles.", "gemini": "BỐI CẢNH VÀ MỤC TIÊU Xơ phổi vô căn (IPF) là bệnh viêm phổi kẽ vô căn phổ biến nhất và có tiên lượng xấu. Bằng chứng dịch tễ học cho thấy những bệnh nhân mắc IPF có nguy cơ phát triển ung thư phổi cao hơn. Kháng nguyên phôi thai (CEA) có liên quan chặt chẽ với ác tính biểu mô. Mục tiêu của nghiên cứu này là đánh giá nồng độ CEA trong huyết thanh ở những bệnh nhân mắc IPF và thực hiện tương quan với chức năng phổi. PHƯƠNG PHÁP Nồng độ CEA trong huyết thanh được đo bằng xét nghiệm miễn dịch phát quang tuần tự tại hai vị trí ở 41 bệnh nhân không hút thuốc mắc IPF. Những bệnh nhân có tiền sử mắc bệnh ác tính ở đường tiêu hóa hoặc các rối loạn khác được biết là có liên quan đến việc tăng CEA trong huyết thanh đã bị loại trừ. KẾT QUẢ Tổng cộng có 41 bệnh nhân được đánh giá. Độ tuổi trung bình (± độ lệch chuẩn) của bệnh nhân là 73 ± 7 tuổi. Sức sống phổi gắng sức trung bình (± độ lệch chuẩn) là 88 ± 20% so với dự đoán và hệ số khuếch tán trung bình (± độ lệch chuẩn) đối với carbon monoxide (DLco) là 52 ± 19% so với dự đoán. Hai mươi mốt (51%) bệnh nhân có nồng độ CEA trong huyết thanh cao hơn giới hạn trên của phạm vi bình thường (0-5 ng/mL). Nồng độ CEA có tương quan âm đáng kể với chức năng phổi (P = 0,005; R(2) = 0,20 đối với sức sống phổi gắng sức và P = 0,006; R(2) = 0,20 đối với DLco). Mức CEA tăng cũng tương quan đáng kể với mức độ xơ hóa. Một mẫu sinh thiết phổi từ một bệnh nhân mắc IPF cho thấy nhuộm màu CEA mạnh ở biểu mô hóa sản lót các nang hình tổ ong và các tiểu phế quản hô hấp. KẾT LUẬN Nồng độ CEA trong huyết thanh tăng cao ở khoảng một nửa số bệnh nhân mắc IPF và có tương quan với mức độ nghiêm trọng của bệnh. Nhuộm miễn dịch mô học cho thấy CEA định vị ở biểu mô hóa sản lót các tiểu phế quản hình tổ ong."} +{"idx": 1980080, "en": "Repeated administration of partial agonist of benzodiazepine receptors Ro 19-8022 (a derivative of quinolizine class) does not elicit withdrawal in adult rats. Our older data demonstrated that single injection of Ro 19-2088 to immature rats induces increased sensitivity to convulsant action of pentylenetetrazol as a withdrawal phenomenon. To know if repeated administration of the partial agonist has the same effect we injected rats at postnatal days 7 to 11 with an anticonvulsant dose of Ro 19-8022 (0.5 mg/kg i.p.) and tested them 24 h, 48 h and 4 days after the last injection. Repeated administration of Ro 19-8022 resulted also in an increased sensitivity to convulsant action of pentylenetetrazol in immature rats (higher incidence and severity of seizures). This effect was significant 24 h after the last injection but only outlined 48 h after administration. No signs of hypersensitivity were seen at 4-day interval. There is a difference between immature and adult brain in an appearance of withdrawal symptom after administration of the partial agonist of benzodiazepine receptors Ro 19-8022.", "gemini": "Việc dùng nhiều lần chất chủ vận một phần của thụ thể benzodiazepine Ro 19-8022 (một dẫn xuất của lớp quinolizine) không gây ra tình trạng cai ở chuột trưởng thành. Dữ liệu trước đây của chúng tôi chứng minh rằng tiêm một lần Ro 19-2088 cho chuột chưa trưởng thành gây ra tình trạng nhạy cảm tăng lên với tác dụng gây co giật của pentylenetetrazol như một hiện tượng cai. Để biết liệu việc dùng nhiều lần chất chủ vận một phần có tác dụng tương tự hay không, chúng tôi đã tiêm cho chuột từ ngày thứ 7 đến ngày thứ 11 sau sinh bằng một liều chống co giật của Ro 19-8022 (0,5 mg/kg i.p.) và thử nghiệm chúng sau 24 giờ, 48 giờ và 4 ngày sau lần tiêm cuối cùng. Việc dùng nhiều lần Ro 19-8022 cũng dẫn đến tình trạng nhạy cảm tăng lên với tác dụng gây co giật của pentylenetetrazol ở chuột chưa trưởng thành (tỷ lệ và mức độ nghiêm trọng của các cơn động kinh cao hơn). Tác dụng này có ý nghĩa sau 24 giờ sau lần tiêm cuối cùng nhưng chỉ được phác thảo sau 48 giờ sau khi dùng. Không có dấu hiệu quá mẫn nào được thấy ở khoảng thời gian 4 ngày. Có sự khác biệt giữa não chưa trưởng thành và não trưởng thành về sự xuất hiện của triệu chứng cai sau khi dùng chất chủ vận một phần của thụ thể benzodiazepine Ro 19-8022."} +{"idx": 1980081, "en": "Adeno-associated virus (AAV)-based vectors have gained increasing attention as gene delivery vehicles in basic and preclinical studies as well as in human gene therapy trials. Especially for the latter two-for both safety and therapeutic efficacy reasons-a detailed characterization of all relevant parameters of the vector preparation is essential. Two important parameters that are routinely used to analyze recombinant AAV vectors are (1) the titer of viral particles containing a (recombinant) viral genome and (2) the purity of the vector preparation, most commonly assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining. An important, third parameter, the titer of total viral particles, that is, the combined titer of both genome-containing and empty viral capsids, is rarely determined. Here, we describe a simple and inexpensive method that allows the simultaneous assessment of both vector purity and the determination of the total viral particle titer. This method, which was validated by comparison with established methods to determine viral particle titers, is based on the fact that Coomassie Brilliant Blue, when bound to proteins, fluoresces in the infrared spectrum. Viral samples are separated by SDS-PAGE followed by Coomassie Brilliant Blue staining and gel analysis with an infrared laser-scanning device. In combination with a protein standard, our method allows the rapid and accurate determination of viral particle titers simultaneously with the assessment of vector purity.", "gemini": "Các vectơ dựa trên virus liên kết với adeno (AAV) ngày càng được quan tâm như là các phương tiện đưa gen trong các nghiên cứu cơ bản và tiền lâm sàng cũng như trong các thử nghiệm liệu pháp gen trên người. Đặc biệt đối với hai lý do sau - vì lý do an toàn và hiệu quả điều trị - việc mô tả chi tiết tất cả các thông số có liên quan của chế phẩm vectơ là rất cần thiết. Hai thông số quan trọng thường được sử dụng để phân tích các vectơ AAV tái tổ hợp là (1) nồng độ hạt virus chứa bộ gen virus (tái tổ hợp) và (2) độ tinh khiết của chế phẩm vectơ, thường được đánh giá bằng phương pháp điện di gel polyacrylamide dodecyl sulfat natri (SDS-PAGE) sau đó nhuộm bạc. Một thông số thứ ba quan trọng, nồng độ của tổng số hạt virus, tức là nồng độ kết hợp của cả các vỏ bọc virus chứa bộ gen và rỗng, hiếm khi được xác định. Ở đây, chúng tôi mô tả một phương pháp đơn giản và không tốn kém cho phép đánh giá đồng thời cả độ tinh khiết của vectơ và xác định nồng độ tổng số hạt virus. Phương pháp này, đã được xác nhận bằng cách so sánh với các phương pháp đã được thiết lập để xác định nồng độ hạt virus, dựa trên thực tế là Coomassie Brilliant Blue, khi liên kết với protein, sẽ phát huỳnh quang trong quang phổ hồng ngoại. Các mẫu virus được tách bằng SDS-PAGE sau đó nhuộm Coomassie Brilliant Blue và phân tích gel bằng thiết bị quét laser hồng ngoại. Kết hợp với một tiêu chuẩn protein, phương pháp của chúng tôi cho phép xác định nhanh chóng và chính xác nồng độ hạt virus đồng thời với việc đánh giá độ tinh khiết của vectơ."} +{"idx": 1980082, "en": "Alzheimer's disease (AD) is the most common progressive age-related dementia in the elderly and the fourth major cause of disability and mortality in that population. The disease is pathologically characterized by deposition of β-amyloid plaques neurofibrillary tangles in the brain. Current strategies for the treatment of AD are symptomatic only. As such, they are less than efficacious in terms of significantly slowing or halting the underlying pathophysiological progression of the disease. Modulation by cell therapy may be new promising disease-modifying therapy. Recently, we showed reduction in amyloid-β (Aβ) levels/β-amyloid plaques and associated astrocytosis following low-dose infusions of mononuclear human umbilical cord blood cells (HUCBCs). Our current study extended our previous findings by examining cognition via (1) the rotarod test, (2) a 2-day version of the radial-arm water maze test, and (3) a subsequent observation in an open pool platform test to characterize the effects of monthly peripheral HUCBC infusion (1×10(6) cells/μL) into the transgenic PSAPP mouse model of cerebral amyloidosis (bearing mutant human APP and presenilin-1 transgenes) from 6 to 12 months of age. We show that HUCBC therapy correlates with decreased (1) cognitive impairment, (2) Aβ levels/β-amyloid plaques, (3) amyloidogenic APP processing, and (4) reactive microgliosis after a treatment of 6 or 10 months. As such, this report lays the groundwork for an HUCBC therapy as potentially novel alternative to oppose AD at the disease-modifying level.", "gemini": "Bệnh Alzheimer (AD) là chứng mất trí liên quan đến tuổi tiến triển phổ biến nhất ở người cao tuổi và là nguyên nhân chính thứ tư gây ra tình trạng khuyết tật và tử vong trong nhóm dân số đó. Về mặt bệnh học, bệnh này được đặc trưng bởi sự lắng đọng các mảng β-amyloid và các đám rối sợi thần kinh trong não. Các chiến lược hiện tại để điều trị bệnh AD chỉ mang tính triệu chứng. Do đó, chúng ít hiệu quả hơn về mặt làm chậm đáng kể hoặc ngăn chặn sự tiến triển bệnh lý sinh học tiềm ẩn của bệnh. Điều chỉnh bằng liệu pháp tế bào có thể là liệu pháp sửa đổi bệnh mới đầy hứa hẹn. Gần đây, chúng tôi đã chứng minh được sự giảm nồng độ amyloid-β (Aβ)/các mảng β-amyloid và tình trạng tế bào hình sao liên quan sau khi truyền liều thấp các tế bào máu cuống rốn người đơn nhân (HUCBC). Nghiên cứu hiện tại của chúng tôi mở rộng các phát hiện trước đó của chúng tôi bằng cách kiểm tra nhận thức thông qua (1) thử nghiệm rotarod, (2) phiên bản kéo dài 2 ngày của thử nghiệm mê cung nước cánh tay hướng tâm và (3) quan sát tiếp theo trong thử nghiệm nền tảng bể bơi mở để mô tả tác dụng của truyền HUCBC ngoại vi hàng tháng (1×10(6) tế bào/μL) vào mô hình chuột PSAPP chuyển gen của chứng bệnh não do tinh bột (mang gen APP đột biến của người và gen chuyển gen presenilin-1) từ 6 đến 12 tháng tuổi. Chúng tôi chứng minh rằng liệu pháp HUCBC tương quan với sự giảm (1) suy giảm nhận thức, (2) nồng độ Aβ/các mảng β-amyloid, (3) quá trình xử lý APP sinh amyloid và (4) tình trạng vi glia phản ứng sau khi điều trị 6 hoặc 10 tháng. Do đó, báo cáo này đặt nền tảng cho liệu pháp HUCBC như một phương pháp thay thế mới có khả năng chống lại bệnh AD ở cấp độ sửa đổi bệnh."} +{"idx": 1980083, "en": "PURPOSE To validate the activities and participation components of The International Classification of Functioning, Disability and Health (ICF). METHODS In this cross-sectional study, 113 Finnish community-dwelling persons with MS were assessed using a semi-structured interview provided by the Canadian Occupational Performance Measure (COPM) to capture participants' self-perceived problems in everyday activities and participation. Problems were linked to the ICF categories. RESULTS Participants identified 527 of the most important occupational performance problems. They covered all chapters of the ICF Activities and Participation components. Forty-one categories out of a total 53 ICF activities and participation categories of the Comprehensive ICF Core Set and four out of five categories of the Brief ICF Core Set were reported on by the participants. The most common category in this sample, 'd920 Recreation and leisure' (145 problems/27.5%), is not included in the Brief ICF Core Set. CONCLUSIONS Most, but not all, ICF activities and participation categories of the ICF Core Sets for MS could be confirmed from the perspective of persons with MS. It is worth considering to add category 'd920 Recreation and leisure' to the Brief ICF Core Set.", "gemini": "MỤC ĐÍCH Xác thực các thành phần hoạt động và tham gia của Phân loại quốc tế về chức năng, khuyết tật và sức khỏe (ICF). PHƯƠNG PHÁP Trong nghiên cứu cắt ngang này, 113 người mắc bệnh đa xơ cứng sống trong cộng đồng ở Phần Lan đã được đánh giá bằng cách sử dụng một cuộc phỏng vấn bán cấu trúc do Đo lường hiệu suất nghề nghiệp của Canada (COPM) cung cấp để nắm bắt các vấn đề mà người tham gia tự nhận thấy trong các hoạt động và sự tham gia hàng ngày. Các vấn đề được liên kết với các danh mục ICF. KẾT QUẢ Những người tham gia đã xác định 527 vấn đề về hiệu suất nghề nghiệp quan trọng nhất. Họ bao gồm tất cả các chương của các thành phần Hoạt động và Tham gia của ICF. Bốn mươi mốt danh mục trong tổng số 53 danh mục hoạt động và tham gia của ICF trong Bộ lõi ICF Toàn diện và bốn trong số năm danh mục của Bộ lõi ICF Tóm tắt đã được những người tham gia báo cáo. Danh mục phổ biến nhất trong mẫu này, 'd920 Giải trí và thư giãn' (145 vấn đề/27,5%), không được đưa vào Bộ lõi ICF Tóm tắt. KẾT LUẬN Hầu hết, nhưng không phải tất cả, các danh mục hoạt động và tham gia của ICF trong Bộ lõi ICF dành cho bệnh đa xơ cứng có thể được xác nhận từ góc độ của những người mắc bệnh đa xơ cứng. Cần cân nhắc thêm danh mục 'd920 Giải trí và thư giãn' vào Bộ lõi ICF Tóm tắt."} +{"idx": 1980084, "en": "BACKGROUND Nurse preceptors are vital linchpins, supporting the transition of new graduates to practicing registered nurses (RNs). This research clarifies similarities and differences among preceptors and nonpreceptors in an established statewide preceptor program. METHODS A secondary analysis of relicensure data from all nurses working in Vermont hospitals in two years (2005 and 2009) was undertaken. RESULTS There were no statistical differences by basic degree type, highest degree, RN experience, years in the current job, job satisfaction, and intention to leave. Statistically significant differences that persisted over time included: (1) being less likely to be employed per diem; (2) work site population density; and (3) being more likely to be enrolled in nursing education programs. CONCLUSION Given the lack of obvious identifiers, organizations would benefit from a systemized approach to preceptor identification and development. Regulators, who hold the clear social and legal mandate for patient safety, must also be an enabling force toward change.", "gemini": "BỐI CẢNH Các cố vấn y tá là những mắt xích quan trọng, hỗ trợ quá trình chuyển đổi từ những sinh viên mới tốt nghiệp thành những y tá hành nghề đã đăng ký (RN). Nghiên cứu này làm rõ những điểm giống và khác nhau giữa các cố vấn và những người không phải cố vấn trong một chương trình cố vấn toàn tiểu bang đã được thiết lập. PHƯƠNG PHÁP Một phân tích thứ cấp về dữ liệu tái cấp phép từ tất cả các y tá làm việc tại các bệnh viện Vermont trong hai năm (2005 và 2009) đã được tiến hành. KẾT QUẢ Không có sự khác biệt thống kê nào theo loại bằng cơ bản, bằng cấp cao nhất, kinh nghiệm RN, số năm làm việc hiện tại, sự hài lòng trong công việc và ý định nghỉ việc. Những khác biệt có ý nghĩa thống kê vẫn tồn tại theo thời gian bao gồm: (1) ít có khả năng được tuyển dụng theo ngày; (2) mật độ dân số tại nơi làm việc; và (3) có nhiều khả năng được ghi danh vào các chương trình giáo dục điều dưỡng. KẾT LUẬN Do thiếu các đặc điểm nhận dạng rõ ràng, các tổ chức sẽ được hưởng lợi từ một cách tiếp cận có hệ thống để xác định và phát triển cố vấn. Các cơ quan quản lý, những người nắm giữ nhiệm vụ pháp lý và xã hội rõ ràng đối với sự an toàn của bệnh nhân, cũng phải là một lực lượng thúc đẩy sự thay đổi."} +{"idx": 1980085, "en": "Advanced practice registered nurses (APRNs) have the unique potential to affect the changing needs of health care in the United States, but are restricted in care provision by varying state regulations and reimbursement policies. Although research shows APRN care to be safe, cost-effective, and of high quality, most medical professional organizations continue to oppose the removal of scope-of-practice barriers, citing patient safety concerns. Nursing organizations at the state and national levels have already begun to invest the time and resources needed for policy change. However, empirical evidence of APRN quality of care must be shared with policymakers, funding entities, and the public. Additionally, support must be garnered from the public and other health care disciplines. Scope-of-practice policy change will occur through the emergence of strong individuals within nursing professional organizations and the joining together of organizations to form one voice.", "gemini": "Các y tá hành nghề nâng cao (APRN) có tiềm năng độc đáo để ảnh hưởng đến những nhu cầu thay đổi của chăm sóc sức khỏe tại Hoa Kỳ, nhưng bị hạn chế trong việc cung cấp dịch vụ chăm sóc do các quy định của tiểu bang và chính sách hoàn trả khác nhau. Mặc dù nghiên cứu cho thấy dịch vụ chăm sóc của APRN là an toàn, hiệu quả về chi phí và chất lượng cao, hầu hết các tổ chức chuyên nghiệp y tế vẫn tiếp tục phản đối việc xóa bỏ các rào cản về phạm vi hành nghề, với lý do lo ngại về sự an toàn của bệnh nhân. Các tổ chức điều dưỡng ở cấp tiểu bang và quốc gia đã bắt đầu đầu tư thời gian và nguồn lực cần thiết cho việc thay đổi chính sách. Tuy nhiên, bằng chứng thực nghiệm về chất lượng chăm sóc của APRN phải được chia sẻ với các nhà hoạch định chính sách, các tổ chức tài trợ và công chúng. Ngoài ra, cần phải nhận được sự hỗ trợ từ công chúng và các chuyên ngành chăm sóc sức khỏe khác. Thay đổi chính sách phạm vi hành nghề sẽ xảy ra thông qua sự xuất hiện của những cá nhân mạnh mẽ trong các tổ chức chuyên nghiệp điều dưỡng và sự liên kết của các tổ chức để tạo thành một tiếng nói."} +{"idx": 1980086, "en": "BACKGROUND Today's clinicians have different levels of knowledge and skill related to evidence-based practice, depending on their educational background, level of experience, and interest. This multidisciplinary study assessed nurses' baseline and posteducation practice, attitudes, and knowledge/skills regarding evidence-based practice. METHODS A descriptive pre- and postsurvey design study evaluated clinical staff's practice, attitudes, and knowledge/skills regarding evidence-based practice with the Clinical Effectiveness and Evidence-Based Practice Questionnaire. RESULTS A total of 327 participants (24%) completed the presurvey and 282 (20%) completed the postsurvey. No statistically significant changes were found in practice, attitudes, and knowledge/skills after the online education. In the multivariate analysis, online education was not a significant predictor of practice, attitudes, or knowledge/skills regarding evidence-based practice; graduate educational degree, formal evidence-based practice classes, and registered nurse status were statistically significant positive predictors. CONCLUSION Administering self-learning online modules may not be the most effective method for expanding evidence-based practice abilities and knowledge/skills of nurses.", "gemini": "BỐI CẢNH Các bác sĩ lâm sàng ngày nay có các mức độ kiến thức và kỹ năng khác nhau liên quan đến thực hành dựa trên bằng chứng, tùy thuộc vào trình độ học vấn, kinh nghiệm và sở thích của họ. Nghiên cứu đa ngành này đã đánh giá thực hành cơ sở và sau giáo dục, thái độ và kiến thức/kỹ năng của các y tá liên quan đến thực hành dựa trên bằng chứng. PHƯƠNG PHÁP Một nghiên cứu thiết kế tiền và hậu khảo sát mô tả đã đánh giá thực hành, thái độ và kiến thức/kỹ năng của đội ngũ lâm sàng liên quan đến thực hành dựa trên bằng chứng bằng Bản câu hỏi về hiệu quả lâm sàng và thực hành dựa trên bằng chứng. KẾT QUẢ Tổng cộng có 327 người tham gia (24%) đã hoàn thành khảo sát trước và 282 người (20%) đã hoàn thành khảo sát sau. Không tìm thấy sự thay đổi có ý nghĩa thống kê nào trong thực hành, thái độ và kiến thức/kỹ năng sau chương trình giáo dục trực tuyến. Trong phân tích đa biến, giáo dục trực tuyến không phải là yếu tố dự đoán có ý nghĩa đối với thực hành, thái độ hoặc kiến thức/kỹ năng liên quan đến thực hành dựa trên bằng chứng; bằng cấp giáo dục sau đại học, các lớp thực hành dựa trên bằng chứng chính thức và trạng thái y tá đã đăng ký là những yếu tố dự đoán tích cực có ý nghĩa thống kê. KẾT LUẬN Việc quản lý các mô-đun trực tuyến tự học có thể không phải là phương pháp hiệu quả nhất để mở rộng khả năng thực hành dựa trên bằng chứng và kiến thức/kỹ năng của các y tá."} +{"idx": 1980087, "en": "OBJECTIVES The effects of Okada Purifying Therapy (OPT), a form of subtle energy (biofield) therapy that originated in Japan, were investigated. Electroencephalograms and the Profile of Mood States scores were measured using a crossover design during OPT and placebo sessions. PARTICIPANTS Nineteen (19) healthy Japanese adults (mean age±standard deviation: 40.8±11.2 years; 10 females) with no previous experience of biofield therapy participated in this study. METHODS Each session lasted 15 minutes. A single-blind, randomized design with a protocol consisting of regular cycles with eyes open followed by eyes closed was used. The power spectral value was calculated in θ (4.0-7.9 Hz), α (8.0-12.9 Hz), and β (13.0-29.9 Hz) frequency ranges. RESULTS The power spectral value of the α band at F(p1), F(p2), F(7), F(z), F(8), C(3), C(z), C(4), and P(z) increased significantly in the OPT session compared with the placebo session. Mood state was improved after both sessions, and no significant difference was found between the two sessions. CONCLUSIONS OPT was more effective in increasing α waves in the frontal and central cortex than a placebo treatment.", "gemini": "MỤC TIÊU Các tác động của Liệu pháp thanh lọc Okada (OPT), một dạng liệu pháp năng lượng tinh tế (biofield) có nguồn gốc từ Nhật Bản, đã được nghiên cứu. Điện não đồ và Điểm số Hồ sơ trạng thái tâm trạng đã được đo bằng thiết kế chéo trong các phiên OPT và giả dược. THAM GIA Mười chín (19) người lớn Nhật Bản khỏe mạnh (tuổi trung bình ± độ lệch chuẩn: 40,8 ± 11,2 tuổi; 10 nữ) chưa từng có kinh nghiệm về liệu pháp biofield đã tham gia nghiên cứu này. PHƯƠNG PHÁP Mỗi phiên kéo dài 15 phút. Một thiết kế ngẫu nhiên, mù đơn với giao thức bao gồm các chu kỳ đều đặn với mắt mở theo sau là mắt nhắm đã được sử dụng. Giá trị phổ công suất được tính trong các dải tần θ (4,0-7,9 Hz), α (8,0-12,9 Hz) và β (13,0-29,9 Hz). KẾT QUẢ Giá trị phổ công suất của dải α tại F (p1), F (p2), F (7), F (z), F (8), C (3), C (z), C (4) và P (z) tăng đáng kể trong phiên OPT so với phiên giả dược. Trạng thái tâm trạng được cải thiện sau cả hai phiên và không có sự khác biệt đáng kể nào được tìm thấy giữa hai phiên. KẾT LUẬN OPT có hiệu quả hơn trong việc tăng sóng α ở vỏ não trước trán và trung tâm so với phương pháp điều trị giả dược."} +{"idx": 1980088, "en": "OBJECTIVES The purpose of this investigation was to analyze epidemiological patterns, clinical features and the management of odontogenic infections in patients undergoing treatment in a dental emergency outpatient care unit. STUDY DESIGN A retrospective analysis of 58 161 case records of patients presenting to an emergency outpatient unit in Hamburg, Germany between 2000-2007 was performed. From this pool, patients with odontogenic infections were identified using an ICD-10 code, analyzing age, gender, medical co-morbidities, duration of pain, ratio of infiltrates/abscesses, affected teeth, management of infection and administered antibiotics. RESULTS Of the 58 161 patients, 5357 (9.2%) were identified as having odontogenic infections, with 2689 (50.2%) inflammatory infiltrates and 2668 (49.8%) abscesses. Mean age was 34.8 ± 16.8 years. As the primary site of odontogenic infection, the most significantly affected teeth were the maxillary and mandibular first molars. Patients in age-group 20-29 years (25.1%) utilized the emergency care unit more frequently than other age groups. Clindamycin was the most frequently administered antibiotic. CONCLUSIONS Early recognition, diagnosis and management of odontogenic infections are requisite for avoiding or minimizing the development of potential complications. Strategies and evidence-based protocols should be developed within the dental ambulatory care sector, advancing interdisciplinary cooperation between general dentists and oral or maxillofacial surgeons.", "gemini": "MỤC TIÊU Mục đích của cuộc điều tra này là phân tích các mô hình dịch tễ học, các đặc điểm lâm sàng và cách quản lý các bệnh nhiễm trùng răng miệng ở những bệnh nhân đang điều trị tại đơn vị cấp cứu nha khoa ngoại trú. THIẾT KẾ NGHIÊN CỨU Một phân tích hồi cứu về 58.161 hồ sơ bệnh án của những bệnh nhân đến khám tại một khoa cấp cứu ngoại trú ở Hamburg, Đức trong giai đoạn 2000-2007 đã được thực hiện. Từ nhóm này, những bệnh nhân bị nhiễm trùng răng miệng đã được xác định bằng mã ICD-10, phân tích tuổi, giới tính, bệnh đi kèm về mặt y tế, thời gian đau, tỷ lệ thâm nhiễm / áp xe, răng bị ảnh hưởng, cách quản lý nhiễm trùng và kháng sinh được sử dụng. KẾT QUẢ Trong số 58.161 bệnh nhân, 5357 (9,2%) được xác định là bị nhiễm trùng răng miệng, trong đó có 2689 (50,2%) là thâm nhiễm viêm và 2668 (49,8%) là áp xe. Độ tuổi trung bình là 34,8 ± 16,8 tuổi. Là vị trí chính của nhiễm trùng răng miệng, những chiếc răng bị ảnh hưởng nhiều nhất là răng hàm lớn thứ nhất trên và dưới. Bệnh nhân trong nhóm tuổi 20-29 (25,1%) sử dụng đơn vị cấp cứu thường xuyên hơn các nhóm tuổi khác. Clindamycin là loại kháng sinh được sử dụng thường xuyên nhất. KẾT LUẬN Nhận biết sớm, chẩn đoán và quản lý các bệnh nhiễm trùng răng miệng là điều cần thiết để tránh hoặc giảm thiểu sự phát triển của các biến chứng tiềm ẩn. Các chiến lược và giao thức dựa trên bằng chứng nên được phát triển trong lĩnh vực chăm sóc ngoại trú nha khoa, thúc đẩy sự hợp tác liên ngành giữa các nha sĩ đa khoa và bác sĩ phẫu thuật răng hàm mặt."} +{"idx": 1980089, "en": "Monodisperse 11 nm indium tin oxide (ITO) nanocrystals (NCs) were synthesized by thermal decomposition of indium acetylacetonate, In(acac)(3), and tin bis(acetylacetonate)dichloride, Sn(acac)(2)Cl(2), at 270 °C in 1-octadecene with oleylamine and oleic acid as surfactants. Dispersed in hexane, these ITO NCs were spin-cast on centimeter-wide glass substrates, forming uniform ITO NC assemblies with root-mean-square roughness of 2.9 nm. The assembly thickness was controlled by ITO NC concentrations in hexane and rotation speeds of the spin coater. Via controlled thermal annealing at 300 °C for 6 h under Ar and 5% H(2), the ITO NC assemblies became conductive and transparent with the 146 nm-thick assembly showing 5.2 × 10(-3) Ω·cm (R(s) = 356 Ω/sq) resistivity and 93% transparency in the visible spectral range--the best values ever reported for ITO NC assemblies prepared from solution phase processes. The stable hexane dispersion of ITO NCs was also readily spin-cast on polyimide (T(g) ~360 °C), and the resultant ITO assembly exhibited a comparable conductivity and transparency to the assembly on a glass substrate. The reported synthesis and assembly provide a promising solution to the fabrication of transparent and conducting ITO NCs on flexible substrates for optoelectronic applications.", "gemini": "Các tinh thể nano oxit thiếc indium (ITO) monodisperse 11 nm (NC) được tổng hợp bằng cách phân hủy nhiệt của indium acetylacetonate, In (acac) (3) và thiếc bis (acetylacetonate) dichloride, Sn (acac) (2) Cl (2), ở 270 ° C trong 1-octadecene với oleylamine và axit oleic là chất hoạt động bề mặt. Phân tán trong hexane, các NC ITO này được quay ly tâm trên các chất nền thủy tinh rộng một cm, tạo thành các cụm NC ITO đồng nhất với độ nhám trung bình của căn bậc hai là 2,9 nm. Độ dày của cụm được kiểm soát bởi nồng độ NC ITO trong hexane và tốc độ quay của máy quay ly tâm. Thông qua quá trình ủ nhiệt có kiểm soát ở 300 ° C trong 6 giờ dưới Ar và 5% H (2), các cụm NC ITO trở nên dẫn điện và trong suốt với cụm dày 146 nm cho thấy điện trở suất 5,2 × 10 (-3) Ω · cm (R (s) = 356 Ω / sq) và độ trong suốt 93% trong dải quang phổ khả kiến - các giá trị tốt nhất từng được báo cáo cho các cụm NC ITO được chế tạo từ các quy trình pha dung dịch. Phân tán hexane ổn định của NC ITO cũng được quay ly tâm dễ dàng trên polyimide (T (g) ~ 360 ° C) và cụm ITO kết quả cho thấy độ dẫn điện và độ trong suốt tương đương với cụm trên chất nền thủy tinh. Quá trình tổng hợp và lắp ráp được báo cáo cung cấp một giải pháp đầy hứa hẹn cho việc chế tạo các NC ITO trong suốt và dẫn điện trên các chất nền linh hoạt cho các ứng dụng quang điện tử."} +{"idx": 1980090, "en": "The first direct borylation of a C-H bond at the 4-position of pyrene was achieved using [Ir(COD)Cl](2)/dtbpy as the catalyst precursor and B(2)pin(2) as the boron source. The position-related photophysical properties of pyrene derivatives are reported.", "gemini": "Phản ứng borylation trực tiếp đầu tiên của liên kết C-H ở vị trí 4 của pyrene đã đạt được bằng cách sử dụng [Ir(COD)Cl](2)/dtbpy làm tiền chất xúc tác và B(2)pin(2) làm nguồn boron. Các tính chất quang lý liên quan đến vị trí của các dẫn xuất pyrene được báo cáo."} +{"idx": 1980091, "en": "AIM Growing numbers of newborns are saved from HIV infection through increased access to mother-to-child transmission prevention programmes. The maternally derived humoral immunity of these children might be impaired, both in terms of quantity and in terms of quality, with consequences for the timing of immunization against measles. METHODS A cell-ELISA technique compared the neutralizing activity on Edmonston strain measles virus of sera from 1- to 4-month-old infants. Ten serum specimens came from noninfected infants of HIV-infected mothers and another 10 from infants of healthy mothers. The sera were matched for the level of conventional ELISA measles antibodies. RESULTS Reflecting infection of the Vero cells by non-neutralized virus, optical density values were significantly higher for the sera from the children of the HIV-infected mothers than for those of the noninfected mothers (p < 0.001). CONCLUSION Maternally derived protection against measles may be impaired by the mother's HIV infection, relating to the quality rather than to the quantity of transplacental antibodies. Selective, early immunization with live attenuated measles vaccine should be evaluated in noninfected children of HIV-1-infected mothers.", "gemini": "AIM Ngày càng có nhiều trẻ sơ sinh được cứu khỏi nhiễm HIV nhờ tăng cường tiếp cận các chương trình phòng ngừa lây truyền từ mẹ sang con. Miễn dịch dịch thể có nguồn gốc từ mẹ của những trẻ này có thể bị suy yếu, cả về số lượng và chất lượng, với hậu quả là thời điểm tiêm chủng vắc-xin phòng ngừa bệnh sởi. PHƯƠNG PHÁP Kỹ thuật tế bào ELISA so sánh hoạt tính trung hòa trên vi-rút sởi chủng Edmonston của huyết thanh từ trẻ sơ sinh từ 1 đến 4 tháng tuổi. Mười mẫu huyết thanh lấy từ trẻ sơ sinh không bị nhiễm của các bà mẹ nhiễm HIV và 10 mẫu khác từ trẻ sơ sinh của các bà mẹ khỏe mạnh. Các huyết thanh được ghép nối với mức độ kháng thể sởi ELISA thông thường. KẾT QUẢ Phản ánh sự nhiễm trùng của các tế bào Vero do vi-rút không trung hòa, giá trị mật độ quang học cao hơn đáng kể đối với huyết thanh từ trẻ em của các bà mẹ nhiễm HIV so với huyết thanh của các bà mẹ không nhiễm HIV (p < 0,001). KẾT LUẬN Sự bảo vệ có nguồn gốc từ mẹ chống lại bệnh sởi có thể bị suy yếu do nhiễm HIV của mẹ, liên quan đến chất lượng chứ không phải số lượng kháng thể nhau thai. Nên đánh giá việc tiêm chủng sớm có chọn lọc bằng vắc-xin sởi giảm độc lực sống ở trẻ em không bị nhiễm của những bà mẹ nhiễm HIV-1."} +{"idx": 1980092, "en": "The term Postpolio Syndrome (PPS) is used to describe new and late manifestations of poliomyelitis that occur later in life in polio survivors. Polio had been eradicated in the United Kingdom (UK) and most of Europe, although this is not the case in all countries. Research in this area has tended to focus upon the impact of polio and PPS on health status and functional health rather than its overall effect on people's lives. This study's two main aims were to explore the ways in which polio and PPS in the UK has affected the respondents' lives and to ascertain their views about how the quality of life could be improved. The two questions were as follows: (1) How has the health of people with polio and PPS affected their quality of life? (2) What would people with polio and PPS change to improve their quality of life? Deductive content analysis using existing qualitative data from a cross-sectional survey of 336 returned questionnaires from persons with polio and PPS was carried out. The average age of the participants was 54 years. Our research found that polio survivors valued social occupations and participation in family life. Our research has also shown that healthcare professionals still do not understand polio and PPS and this lack of understanding influences their clients' quality of life. Finances and accessibility of environments also influence participation in chosen occupations. Rehabilitation programmes for people with polio and PPS need to be targeted towards maintaining and improving accessible environments and participation in chosen occupations, and healthcare professionals need to ensure that persons with polio and PPS are referred to persons with specific expertise in this area.", "gemini": "Thuật ngữ Hội chứng hậu bại liệt (PPS) được sử dụng để mô tả các biểu hiện mới và muộn của bệnh bại liệt xảy ra ở những người sống sót sau bại liệt. Bệnh bại liệt đã được xóa sổ ở Vương quốc Anh (UK) và hầu hết các nước Châu Âu, mặc dù không phải ở tất cả các quốc gia. Nghiên cứu trong lĩnh vực này có xu hướng tập trung vào tác động của bệnh bại liệt và PPS đối với tình trạng sức khỏe và sức khỏe chức năng hơn là tác động tổng thể của nó đối với cuộc sống của mọi người. Hai mục tiêu chính của nghiên cứu này là khám phá những cách mà bệnh bại liệt và PPS ở Vương quốc Anh đã ảnh hưởng đến cuộc sống của những người trả lời và để xác định quan điểm của họ về cách cải thiện chất lượng cuộc sống. Hai câu hỏi như sau: (1) Sức khỏe của những người mắc bệnh bại liệt và PPS đã ảnh hưởng đến chất lượng cuộc sống của họ như thế nào? (2) Những người mắc bệnh bại liệt và PPS sẽ thay đổi những gì để cải thiện chất lượng cuộc sống của họ? Phân tích nội dung diễn dịch sử dụng dữ liệu định tính hiện có từ một cuộc khảo sát cắt ngang gồm 336 bảng câu hỏi đã trả lời từ những người mắc bệnh bại liệt và PPS đã được thực hiện. Độ tuổi trung bình của những người tham gia là 54 tuổi. Nghiên cứu của chúng tôi phát hiện ra rằng những người sống sót sau bệnh bại liệt coi trọng các công việc xã hội và sự tham gia vào cuộc sống gia đình. Nghiên cứu của chúng tôi cũng chỉ ra rằng các chuyên gia chăm sóc sức khỏe vẫn chưa hiểu về bệnh bại liệt và PPS và sự thiếu hiểu biết này ảnh hưởng đến chất lượng cuộc sống của khách hàng của họ. Tài chính và khả năng tiếp cận môi trường cũng ảnh hưởng đến sự tham gia vào các nghề đã chọn. Các chương trình phục hồi chức năng cho những người mắc bệnh bại liệt và PPS cần hướng đến mục tiêu duy trì và cải thiện môi trường dễ tiếp cận và tham gia vào các nghề đã chọn, và các chuyên gia chăm sóc sức khỏe cần đảm bảo rằng những người mắc bệnh bại liệt và PPS được giới thiệu đến những người có chuyên môn cụ thể trong lĩnh vực này."} +{"idx": 1980093, "en": "Photodynamic therapy (PDT) has been originally developed for cancer treatment, but recently, it has been successfully employed against microorganisms, including fungi. Chromoblastomycosis is a subcutaneous fungal infection that is recalcitrant to conventional antifungal drug therapy. The most frequent species involved are Foncecaea pedrosoi and Cladophialophora carrionii. The present study aimed to verify the efficacy in vitro of PDT employing methylene blue (MB) as a photosensitiser and Light emmiting diode (LED) (InGaAl) as the light source. Methylene blue at the concentrations of 16, 32 and 64 μg/mL and LED (InGalP) were employed for 15 min against spores of two isolates of F. pedrosoi and two isolates of C. carrionii. The spores were plated on Sabouraud Dextrose agar and the number of colony forming units was counted after 7-10 days of incubation at 37 °C. The PDT with MB and LED was efficient in reducing the growth of all samples tested. Better results were obtained for the concentration of 32 μg/mL of MB. The treatment proved to be highly effective in killing the samples of F. pedrosoi and Cladophialophora pedrosoi tested in vitro. PDT arises as a promising alternative for the treatment of this subcutaneous infection.", "gemini": "Liệu pháp quang động (PDT) ban đầu được phát triển để điều trị ung thư, nhưng gần đây, nó đã được sử dụng thành công để chống lại các vi sinh vật, bao gồm cả nấm. Chromoblastomycosis là một bệnh nhiễm nấm dưới da rất khó điều trị bằng thuốc chống nấm thông thường. Các loài thường gặp nhất là Foncecaea pedrosoi và Cladophialophora carrionii. Nghiên cứu hiện tại nhằm xác minh hiệu quả trong ống nghiệm của PDT sử dụng xanh methylen (MB) như một chất nhạy sáng và điốt phát sáng (LED) (InGaAl) làm nguồn sáng. Xanh methylen ở nồng độ 16, 32 và 64 μg/mL và LED (InGalP) được sử dụng trong 15 phút để chống lại bào tử của hai phân lập F. pedrosoi và hai phân lập C. carrionii. Các bào tử được cấy trên thạch Sabouraud Dextrose và số đơn vị hình thành khuẩn lạc được đếm sau 7-10 ngày ủ ở 37 °C. PDT với MB và LED có hiệu quả trong việc giảm sự phát triển của tất cả các mẫu được thử nghiệm. Kết quả tốt hơn thu được ở nồng độ 32 μg/mL MB. Phương pháp điều trị này tỏ ra rất hiệu quả trong việc tiêu diệt các mẫu F. pedrosoi và Cladophialophora pedrosoi được thử nghiệm trong ống nghiệm. PDT xuất hiện như một phương pháp thay thế đầy hứa hẹn cho việc điều trị nhiễm trùng dưới da này."} +{"idx": 1980094, "en": "AIM Sarcopenia-related falls and fractures are becoming an emerging problem as a result of rapid aging worldwide. We aimed to investigate the prevalence of sarcopenia by estimating the muscle mass of the arms and legs of patients with and without hip fracture. METHODS This cross-sectional study examined 357 patients immediately after a hip fracture (the HF group) and in 2511 patients from an outpatient clinic who did not have a hip fracture (the NF group) at single institution in Japan. We carried out whole-body dual energy X-ray absorptiometry to analyze body composition with skeletal muscle mass index (SMI; lean mass/height(2)) and bone mineral density (BMD). We carried out stepwise logistic regression analysis to determine the factors associated with a hip fracture. RESULTS Lower appendicular SMI (P < 0.001), leg SMI (P < 0.001), and higher prevalence of sarcopenia (P < 0.001) were observed in the HF group after controlling for age and sex. The arm SMI was similar in both groups (P > 0.95). In multivariate analysis, the presence of sarcopenia, older age and lower BMD were associated with the occurrence of a hip fracture (OR 1.476, P = 0.002; OR 1.103, P < 0.001; OR 0.082, P < 0.001; respectively). CONCLUSION This study showed a higher prevalence of sarcopenia and more reduced leg muscle mass in patients after a hip fracture than in the outclinic patients who did not have hip fractures. The results imply sarcopenia can be a risk factor for a hip fracture.", "gemini": "MỤC TIÊU Ngã và gãy xương liên quan đến chứng teo cơ đang trở thành một vấn đề mới nổi do tình trạng già hóa nhanh chóng trên toàn thế giới. Chúng tôi nhằm mục đích điều tra tỷ lệ mắc chứng teo cơ bằng cách ước tính khối lượng cơ của cánh tay và chân của bệnh nhân có và không bị gãy xương hông. PHƯƠNG PHÁP Nghiên cứu cắt ngang này đã kiểm tra 357 bệnh nhân ngay sau khi bị gãy xương hông (nhóm HF) và 2511 bệnh nhân từ một phòng khám ngoại trú không bị gãy xương hông (nhóm NF) tại một cơ sở duy nhất ở Nhật Bản. Chúng tôi đã tiến hành đo hấp thụ tia X năng lượng kép toàn thân để phân tích thành phần cơ thể với chỉ số khối lượng cơ xương (SMI; khối lượng nạc/chiều cao (2)) và mật độ khoáng xương (BMD). Chúng tôi đã tiến hành phân tích hồi quy logistic từng bước để xác định các yếu tố liên quan đến gãy xương hông. KẾT QUẢ Chỉ số SMI phụ thuộc phụ thuộc thấp hơn (P < 0,001), chỉ số SMI chân (P < 0,001) và tỷ lệ mắc chứng teo cơ cao hơn (P < 0,001) được quan sát thấy ở nhóm HF sau khi kiểm soát tuổi và giới tính. Chỉ số SMI cánh tay tương tự nhau ở cả hai nhóm (P> 0,95). Trong phân tích đa biến, sự hiện diện của chứng teo cơ, tuổi cao hơn và BMD thấp hơn có liên quan đến việc xảy ra gãy xương hông (OR 1,476, P = 0,002; OR 1,103, P < 0,001; OR 0,082, P < 0,001; tương ứng). KẾT LUẬN Nghiên cứu này cho thấy tỷ lệ mắc chứng teo cơ cao hơn và khối lượng cơ chân giảm nhiều hơn ở những bệnh nhân sau khi bị gãy xương hông so với những bệnh nhân ngoại trú không bị gãy xương hông. Kết quả ngụ ý rằng chứng teo cơ có thể là một yếu tố nguy cơ gây gãy xương hông."} +{"idx": 1980095, "en": "OBJECTIVE To examine associations between community residential segregation by income and race/ethnicity, and the supply of federally qualified health centers (FQHCs) in urban areas. DATA SOURCES AND STUDY SETTING Area Resource File (2000-2007) linked with 2000 U.S. Census on U.S. metropolitan counties (N = 1,786). STUDY DESIGN We used logistic and negative binomial regression models with state-level fixed effects to examine how county-level characteristics in 2000 are associated with the presence of FQHCs in 2000, and with the increase in FQHCs from 2000 to 2007. Income and racial/ethnic residential segregation were measured by poverty and the non-white dissimilarity indices, respectively. Covariates included measures of federal criteria for medically underserved areas/populations. PRINCIPAL FINDINGS Counties with a high non-white dissimilarity index and a high percentage of minorities were more likely to have an FQHC in 2000. When we examined the addition of new FQHCs from 2000 to 2007, the effects of both poverty and non-white dissimilarity indices were positive and significant. CONCLUSIONS Residential segregation likely produces geographic segregation of health services, such that provider maldistribution may explain the association between residential segregation and FQHC supply. Metropolitan areas that fail to achieve greater integration of poor and minority communities may require FQHCs to compensate for provider shortages.", "gemini": "MỤC TIÊU Kiểm tra mối liên quan giữa sự phân biệt chủng tộc/dân tộc và thu nhập theo nơi cư trú trong cộng đồng và nguồn cung cấp các trung tâm y tế đủ điều kiện của liên bang (FQHC) ở các khu vực đô thị. NGUỒN DỮ LIỆU VÀ THIẾT LẬP NGHIÊN CỨU Tệp tài nguyên khu vực (2000-2007) được liên kết với Điều tra dân số Hoa Kỳ năm 2000 về các quận đô thị của Hoa Kỳ (N = 1.786). THIẾT KẾ NGHIÊN CỨU Chúng tôi đã sử dụng các mô hình hồi quy logistic và nhị thức âm với các hiệu ứng cố định cấp tiểu bang để kiểm tra cách các đặc điểm cấp quận vào năm 2000 có liên quan đến sự hiện diện của FQHC vào năm 2000 và với sự gia tăng FQHC từ năm 2000 đến năm 2007. Segregate chủng tộc/dân tộc và thu nhập theo nơi cư trú được đo lường bằng các chỉ số nghèo đói và bất đồng về chủng tộc/dân tộc. Các biến phụ bao gồm các biện pháp theo tiêu chí của liên bang đối với các khu vực/dân số thiếu dịch vụ y tế. KẾT QUẢ CHÍNH Các quận có chỉ số bất đồng về chủng tộc/dân tộc cao và tỷ lệ người dân tộc thiểu số cao có nhiều khả năng có FQHC vào năm 2000. Khi chúng tôi kiểm tra việc bổ sung các FQHC mới từ năm 2000 đến năm 2007, các tác động của cả chỉ số nghèo đói và bất đồng về chủng tộc/dân tộc đều là tích cực và có ý nghĩa. KẾT LUẬN Phân biệt chủng tộc theo nơi cư trú có khả năng tạo ra sự phân biệt về mặt địa lý đối với các dịch vụ y tế, do đó sự phân bổ không đều của các nhà cung cấp có thể giải thích mối liên quan giữa sự phân biệt chủng tộc theo nơi cư trú và nguồn cung cấp FQHC. Các khu vực đô thị không đạt được sự hòa nhập lớn hơn của các cộng đồng nghèo và dân tộc thiểu số có thể yêu cầu FQHC để bù đắp cho tình trạng thiếu nhà cung cấp."} +{"idx": 1980096, "en": "We have previously demonstrated that contemporary St. Jude devices (pacemakers and implantable cardioverter-defibrillators [ICDs]; St. Jude Medical, Sylmar, CA, USA) are designed to generate an extended postventricular atrial refractory period (PVARP) of 475 ms at the termination of conventional automatic mode switching (AMS) in response to atrial tachyarrhythmias . This response may cause functional atrial undersensing . A similar PVARP response unrelated to conventional AMS was found in four St. Jude devices (three ICDs and one pacemaker) whenever a nontracking pacing mode switched to a tracking DDD(R) mode. PVARP extension and functional atrial undersensing were observed when the VOO, VVI, and the DDI(R) modes (unrelated to conventional AMS) switched to the DDD(R) mode . In one patient the switch from the OOO mode (in the programmed noise reversion mode) to the DDD mode occurred after cessation of electromagnetic interference disturbing the ventricular channel. In this case PVARP extension was seen only in the corresponding markers because no P waves occurred coincidentally with the extended PVARP. The PVARP extension caused by a mode switch to the tracking function was designed to prevent sensing of a retrograde P wave on the first cycle of the reestablished tracking mode. The observed functional atrial undersensing is a normal manifestation of device function and must not be misinterpreted as a true atrial undersensing problem.", "gemini": "Chúng tôi đã chứng minh trước đây rằng các thiết bị St. Jude hiện đại (máy tạo nhịp tim và máy khử rung tim cấy ghép [ICD]; St. Jude Medical, Sylmar, CA, Hoa Kỳ) được thiết kế để tạo ra thời kỳ trơ trễ nhĩ sau thất kéo dài (PVARP) là 475 ms khi kết thúc chuyển đổi chế độ tự động thông thường (AMS) để đáp ứng với các nhịp nhanh trên thất. Phản ứng này có thể gây ra tình trạng cảm nhận nhĩ không đầy đủ chức năng. Một phản ứng PVARP tương tự không liên quan đến AMS thông thường được tìm thấy ở bốn thiết bị St. Jude (ba ICD và một máy tạo nhịp tim) bất cứ khi nào chế độ tạo nhịp không theo dõi chuyển sang chế độ theo dõi DDD(R). Mở rộng PVARP và cảm nhận nhĩ không đầy đủ chức năng được quan sát thấy khi các chế độ VOO, VVI và DDI(R) (không liên quan đến AMS thông thường) chuyển sang chế độ DDD(R). Ở một bệnh nhân, sự chuyển đổi từ chế độ OOO (ở chế độ đảo ngược nhiễu được lập trình) sang chế độ DDD xảy ra sau khi chấm dứt nhiễu điện từ làm nhiễu kênh thất. Trong trường hợp này, sự mở rộng PVARP chỉ được nhìn thấy ở các dấu hiệu tương ứng vì không có sóng P nào xảy ra trùng hợp với PVARP mở rộng. Sự mở rộng PVARP do chuyển đổi chế độ sang chức năng theo dõi được thiết kế để ngăn chặn việc cảm nhận sóng P ngược dòng ở chu kỳ đầu tiên của chế độ theo dõi được thiết lập lại. Việc cảm nhận nhĩ không đầy đủ chức năng được quan sát thấy là biểu hiện bình thường của chức năng thiết bị và không được hiểu sai là vấn đề cảm nhận nhĩ không đầy đủ thực sự."} +{"idx": 1980097, "en": "AIMS Arabidopsis thaliana l- and d-cysteine desulfhydrases (AtLCD and AtDCD) are two important H(2) S-generating enzymes. This study determined the effects of H(2) S derived from AtLCD and AtDCD on cadmium (Cd) toxicity in Escherichia coli. METHODS AND RESULTS AtLCD and AtDCD were cloned into pET28a vectors and transformed into wild-type E. coli strain BL21(DE3), named BL21(LCD) and BL21(DCD). In the induced BL21(LCD) and BL21(DCD) compared with wild type, significantly higher H(2) S generation rates were observed. Additionally, higher survival rates, reduced contents of malondialdehyde (MDA) and hydrogen peroxide (H(2) O(2)), decreased activities of superoxide dismutase and catalase under 220 μmol l(-1) Cd stress were noted. We obtained similar results in the wild type treated with NaHS, a H(2) S donor. The above changes were substantially counteracted by the mixture of ammonia and pyruvic acid potassium (NH(3) + C(3) H(3) KO(3)), a synthetic inhibitor of H(2) S. CONCLUSIONS AtLCD and AtDCD catalyse the H(2) S production, generating an ameliorating effect against Cd-induced oxidative stress and resulting in E. coli resistance to Cd toxicity. SIGNIFICANCE AND IMPACT OF THE STUDY H(2) S as a gasotransmitter is certified to have an ameliorating effect against Cd toxicity, thus providing information for further research regarding the role of H(2) S in regulating resistance to the heavy metal stress in organisms.", "gemini": "MỤC TIÊU Arabidopsis thaliana l- và d-cysteine desulfhydrases (AtLCD và AtDCD) là hai loại enzyme tạo ra H(2) S quan trọng. Nghiên cứu này xác định tác động của H(2) S có nguồn gốc từ AtLCD và AtDCD đối với độc tính của cadmium (Cd) ở Escherichia coli. PHƯƠNG PHÁP VÀ KẾT QUẢ AtLCD và AtDCD đã được nhân bản vào các vectơ pET28a và chuyển đổi thành chủng E. coli BL21(DE3) kiểu hoang dã, có tên là BL21(LCD) và BL21(DCD). Trong BL21(LCD) và BL21(DCD) được cảm ứng so với kiểu hoang dã, người ta quan sát thấy tỷ lệ tạo ra H(2) S cao hơn đáng kể. Ngoài ra, tỷ lệ sống sót cao hơn, giảm hàm lượng malondialdehyde (MDA) và hydro peroxide (H(2) O(2)), giảm hoạt động của superoxide dismutase và catalase dưới ứng suất Cd 220 μmol l(-1). Chúng tôi thu được kết quả tương tự ở kiểu hoang dã được xử lý bằng NaHS, một chất cho H(2) S. Những thay đổi trên về cơ bản đã được hỗn hợp amoniac và kali pyruvic (NH(3) + C(3) H(3) KO(3)), một chất ức chế tổng hợp của H(2) S, chống lại đáng kể. KẾT LUẬN AtLCD và AtDCD xúc tác quá trình sản xuất H(2) S, tạo ra tác dụng cải thiện đối với stress oxy hóa do Cd gây ra và dẫn đến khả năng chống lại độc tính của Cd ở E. coli. Ý NGHĨA VÀ TÁC ĐỘNG CỦA NGHIÊN CỨU H(2) S dưới dạng khí truyền tin được chứng nhận có tác dụng cải thiện đối với độc tính của Cd, do đó cung cấp thông tin cho các nghiên cứu tiếp theo về vai trò của H(2) S trong việc điều chỉnh khả năng chống lại stress kim loại nặng trong các sinh vật."} +{"idx": 1980098, "en": "Podoplanin is a platelet aggregation-inducing factor associated with tumor metastasis, malignant progression, and cancer stem cells. We produced a rat-human chimeric anti-podoplanin mAb, NZ-8, from rat anti-podoplanin mAb (NZ-1). Although both NZ-1 and NZ-8 possess high binding affinities and high neutralizing activities of platelet aggregation, the antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity of NZ-8 were much higher than NZ-1. Furthermore, both NZ-1 and NZ-8 inhibited the growth of podoplanin-expressing tumors in vivo. Both NZ-1 and NZ-8 also suppressed hematogenous metastasis of podoplanin-expressing tumors. These results suggest that anti-podoplanin mAbs suppressed hematogenous metastasis by both neutralization and antibody-dependent cellular cytotoxicity/complement-dependent cytotoxicity activities. Targeting therapy to podoplanin-expressing tumors should be useful as a novel immunotherapy.", "gemini": "Podoplanin là một yếu tố gây ra kết tập tiểu cầu liên quan đến di căn khối u, tiến triển ác tính và tế bào gốc ung thư. Chúng tôi đã tạo ra một mAb anti-podoplanin chimeric chuột-người, NZ-8, từ mAb anti-podoplanin chuột (NZ-1). Mặc dù cả NZ-1 và NZ-8 đều có ái lực liên kết cao và hoạt tính trung hòa cao đối với kết tập tiểu cầu, nhưng độc tính tế bào phụ thuộc kháng thể và độc tính tế bào phụ thuộc bổ sung của NZ-8 cao hơn nhiều so với NZ-1. Hơn nữa, cả NZ-1 và NZ-8 đều ức chế sự phát triển của các khối u biểu hiện podoplanin trong cơ thể sống. Cả NZ-1 và NZ-8 cũng ức chế di căn qua đường máu của các khối u biểu hiện podoplanin. Những kết quả này cho thấy rằng mAb anti-podoplanin ức chế di căn qua đường máu bằng cả hoạt động trung hòa và hoạt động độc tính tế bào phụ thuộc kháng thể/độc tính tế bào phụ thuộc bổ sung. Liệu pháp nhắm mục tiêu vào các khối u biểu hiện podoplanin nên hữu ích như một phương pháp miễn dịch mới."} +{"idx": 1980099, "en": "BACKGROUND Congenital erythropoietic porphyria (CEP) is an autosomal recessive cutaneous porphyria caused by decreased activity of uroporphyrinogen III synthase (UROS). Its predominant characteristics include bullous cutaneous photosensitivity to visible light from early infancy, progressive photomutilation and chronic haemolytic anaemia. Due to its rarity and genetic heterogeneity, clinical phenotypes are unclear and its impact on health-related quality of life (HRQoL) has not been previously assessed. OBJECTIVES To define comprehensively CEP phenotypes and assess their impact on HRQoL, and to correlate these factors with laboratory parameters. METHODS A single observer assessed patients with CEP from four European countries. RESULTS Twenty-seven unrelated patients with CEP, aged between 7.6 and 65 years, participated in the study. The patients came from the U.K. (17), France (4), Switzerland (4) and Germany (2). Additional data were obtained for two deceased patients. Newly characterized features of CEP include acute-onset cutaneous and noncutaneous symptoms immediately following sunlight exposure, and pink erythematous facial papules. There was a lack of consistent genotype-phenotype correlation in CEP. The main poor prognostic factors in CEP are the early age of disease onset and haematological complications. CONCLUSIONS CEP is a multisystem disease; cutaneous, ocular, oral and skeletal manifestations also contribute to disease severity and impact on HRQoL, in addition to the haematological complications. The rarity of the disease can lead to delayed diagnosis. The lack of consistent genotype-phenotype correlation in CEP suggests a contribution to phenotype from other factors, such as environment, patients' photoprotective behaviour and genes other than UROS. There is currently an unmet need for multidisciplinary management of patients with CEP.", "gemini": "BỐI CẢNH Bệnh porphyria tạo hồng cầu bẩm sinh (CEP) là một bệnh porphyria da do gen lặn trên NST thường gây ra do hoạt động giảm của uroporphyrinogen III synthase (UROS). Đặc điểm nổi bật của bệnh bao gồm nhạy cảm với ánh sáng da có mụn nước đối với ánh sáng nhìn thấy từ thời thơ ấu, tự làm tổn thương da do ánh sáng tiến triển và thiếu máu tan máu mạn tính. Do tính hiếm gặp và tính không đồng nhất về mặt di truyền, kiểu hình lâm sàng không rõ ràng và tác động của bệnh đối với chất lượng cuộc sống liên quan đến sức khỏe (HRQoL) trước đây chưa được đánh giá. MỤC TIÊU Xác định toàn diện các kiểu hình CEP và đánh giá tác động của chúng đối với HRQoL, đồng thời liên hệ các yếu tố này với các thông số xét nghiệm. PHƯƠNG PHÁP Một người quan sát duy nhất đã đánh giá những bệnh nhân mắc CEP từ bốn quốc gia châu Âu. KẾT QUẢ Hai mươi bảy bệnh nhân mắc CEP không có quan hệ họ hàng, trong độ tuổi từ 7,6 đến 65, đã tham gia nghiên cứu. Những bệnh nhân này đến từ Anh (17), Pháp (4), Thụy Sĩ (4) và Đức (2). Dữ liệu bổ sung đã được thu thập đối với hai bệnh nhân đã tử vong. Các đặc điểm mới được xác định của CEP bao gồm các triệu chứng ngoài da và không ngoài da khởi phát cấp tính ngay sau khi tiếp xúc với ánh nắng mặt trời và các sẩn hồng ban đỏ trên mặt. Không có mối tương quan kiểu gen-kiểu hình nhất quán ở CEP. Các yếu tố tiên lượng xấu chính ở CEP là tuổi khởi phát bệnh sớm và các biến chứng về huyết học. KẾT LUẬN CEP là một bệnh đa hệ thống; các biểu hiện trên da, mắt, miệng và xương cũng góp phần làm bệnh nặng hơn và ảnh hưởng đến HRQoL, ngoài các biến chứng về huyết học. Tính hiếm gặp của bệnh có thể dẫn đến chẩn đoán muộn. Việc thiếu mối tương quan kiểu gen-kiểu hình nhất quán ở CEP cho thấy sự đóng góp vào kiểu hình từ các yếu tố khác, chẳng hạn như môi trường, hành vi bảo vệ da của bệnh nhân và các gen khác ngoài UROS. Hiện tại, vẫn chưa đáp ứng được nhu cầu về việc quản lý đa chuyên khoa đối với những bệnh nhân mắc CEP."} +{"idx": 1980100, "en": "To extend past research on interpersonal communication and campaign effects, we hypothesized that anti-drug mass media campaign message exposure indirectly affects visiting anti- and pro-drug websites through targeted parent-child and friend-to-friend communication against drugs, as well as through having drug-related discussions during organized group activities. Second, we posited that engaging in anti-drug interpersonal communication indirectly affects adolescents' drug use through two intervening variables: visiting anti-drug websites and visiting pro-drug websites. Using self-reported longitudinal data from 2,749 youth, we found that as youth reported higher levels of anti-drug mass media campaign message exposure, they were more likely to talk to friends about the bad consequences of drugs, how to avoid drugs, and anti-drug ads. In turn, however, they were more likely to visit pro-drug websites, and subsequently, to smoke cigarettes.", "gemini": "Để mở rộng nghiên cứu trước đây về giao tiếp giữa các cá nhân và hiệu ứng chiến dịch, chúng tôi đưa ra giả thuyết rằng việc tiếp xúc với thông điệp chiến dịch truyền thông đại chúng chống ma túy ảnh hưởng gián tiếp đến việc truy cập các trang web ủng hộ và phản đối ma túy thông qua giao tiếp có mục tiêu giữa cha mẹ và con cái và giữa bạn bè với nhau về chống ma túy, cũng như thông qua việc thảo luận về ma túy trong các hoạt động nhóm có tổ chức. Thứ hai, chúng tôi đưa ra giả thuyết rằng việc tham gia giao tiếp giữa các cá nhân về chống ma túy ảnh hưởng gián tiếp đến việc sử dụng ma túy của thanh thiếu niên thông qua hai biến can thiệp: truy cập các trang web chống ma túy và truy cập các trang web ủng hộ ma túy. Sử dụng dữ liệu theo chiều dọc tự báo cáo từ 2.749 thanh thiếu niên, chúng tôi thấy rằng khi thanh thiếu niên báo cáo mức độ tiếp xúc với thông điệp chiến dịch truyền thông đại chúng chống ma túy cao hơn, thì khả năng các em nói chuyện với bạn bè về hậu quả xấu của ma túy, cách tránh ma túy và quảng cáo chống ma túy cao hơn. Tuy nhiên, ngược lại, các em có nhiều khả năng truy cập các trang web ủng hộ ma túy và sau đó là hút thuốc lá."} +{"idx": 1980101, "en": "AIM The present study aimed to clarify the factors affecting early psychiatric intervention for patients with first-episode psychosis in Japan. METHODS A postal questionnaire survey was conducted in 346 members of the Association of Family of Psychosis Patients, in Mie, Japan. RESULTS The questionnaire was completed by 138 respondents. The mean interval between a suspected first episode of psychosis and the first visit to a medical institution was 18.4 months (SD: 40.0, range: 0-336 months). Forty-five respondents consulted a family member first and required a mean duration of 9.8 months (SD: 11.2) before their first visit; this was significantly shorter than those who did not. Eighteen respondents consulted their class teacher at school first and required a mean duration of 40.2 months (SD: 63.0) before their first visit; this was significantly longer (P < 0.05) than those who did not. When loss of sleep or appetite was present, the mean duration before their first visit was 9.3 months (SD: 12.1), which was significantly shorter (P < 0.05) than the time required by those who did not. Logistic regression analysis using the first episode-first visit interval as an independent variable, and independent variables such as age, gender of the patient and family member, and loss of sleep, revealed that variables relating to the respondent's notion about the psychosis were mitigating factors (e.g. 'prejudice-causing' and 'nothing to do with me'). CONCLUSION Prejudice towards psychosis and poor awareness about mental illnesses are two factors that may affect early psychiatric intervention.", "gemini": "MỤC ĐÍCH Nghiên cứu hiện tại nhằm làm rõ các yếu tố ảnh hưởng đến can thiệp tâm thần sớm cho những bệnh nhân mắc chứng loạn thần đầu tiên ở Nhật Bản. PHƯƠNG PHÁP Một cuộc khảo sát bằng phiếu hỏi qua thư đã được tiến hành ở 346 thành viên của Hiệp hội Gia đình bệnh nhân loạn thần tại Mie, Nhật Bản. KẾT QUẢ Có 138 người trả lời đã hoàn thành bảng câu hỏi. Khoảng thời gian trung bình giữa một đợt loạn thần đầu tiên nghi ngờ và lần khám đầu tiên tại cơ sở y tế là 18,4 tháng (SD: 40,0, phạm vi: 0-336 tháng). Bốn mươi lăm người trả lời đã tham khảo ý kiến của một thành viên gia đình trước và cần thời gian trung bình là 9,8 tháng (SD: 11,2) trước lần khám đầu tiên; khoảng thời gian này ngắn hơn đáng kể so với những người không tham khảo ý kiến. Mười tám người trả lời đã tham khảo ý kiến của giáo viên chủ nhiệm tại trường trước và cần thời gian trung bình là 40,2 tháng (SD: 63,0) trước lần khám đầu tiên; khoảng thời gian này dài hơn đáng kể (P <0,05) so với những người không tham khảo ý kiến. Khi mất ngủ hoặc chán ăn, thời gian trung bình trước lần khám đầu tiên là 9,3 tháng (SD: 12,1), ngắn hơn đáng kể (P <0,05) so với thời gian cần thiết của những người không bị mất ngủ hoặc chán ăn. Phân tích hồi quy logistic sử dụng khoảng thời gian đợt đầu tiên-lần khám đầu tiên làm biến độc lập và các biến độc lập như tuổi, giới tính của bệnh nhân và thành viên gia đình, mất ngủ cho thấy các biến liên quan đến quan niệm của người trả lời về chứng loạn thần là các yếu tố làm giảm nhẹ (ví dụ: 'gây định kiến' và 'không liên quan đến tôi'). KẾT LUẬN Định kiến đối với chứng loạn thần và nhận thức kém về các bệnh tâm thần là hai yếu tố có thể ảnh hưởng đến việc can thiệp tâm thần sớm."} +{"idx": 1980102, "en": "Fungal cell adhesion molecules are critical for the attachment of cells to each other and to surfaces and in pathogens contribute to virulence. Fungal adhesins are typically heavily glycosylated. The impact of protein glycosylation on the function and regulation of adhesion glycoproteins is not clear. We examined the role of protein glycosylation on the adherence properties of the major adhesion molecule Muc1/Flo11 in the budding yeast Saccharomyces cerevisiae. Using a conditional mutant required for an early step in protein glycosylation, pmi40-101, we show that the glycosylation of Flo11 is required for invasive growth and biofilm/mat formation. Underglycosylated Flo11 was not defective in cell-surface localization or binding to wild-type cells in trans. However, wild-type Flo11 was defective for binding to the surface of cells undergoing a glycosylation stress. Shed Flo11 and other shed glycoproteins (Msb2 and Hkr1) were extremely stable with half-lives on the order of days. The glycosylation of Flo11 contributed to its stability. Moreover, the overall balance between Flo11 production, shedding, and turnover favored accumulation of the shed protein over time. Our findings may be applicable to fungal adhesion molecules in other species including pathogens.", "gemini": "Các phân tử bám dính tế bào nấm rất quan trọng đối với sự bám dính của các tế bào với nhau và với các bề mặt và ở các tác nhân gây bệnh góp phần làm tăng độc lực. Các adhesin nấm thường được glycosyl hóa mạnh. Tác động của glycosyl hóa protein lên chức năng và điều hòa của glycoprotein bám dính vẫn chưa rõ ràng. Chúng tôi đã xem xét vai trò của glycosyl hóa protein đối với các đặc tính bám dính của phân tử bám dính chính Muc1/Flo11 trong nấm men nảy chồi Saccharomyces cerevisiae. Sử dụng một đột biến có điều kiện cần thiết cho một bước đầu trong quá trình glycosyl hóa protein, pmi40-101, chúng tôi chỉ ra rằng glycosyl hóa của Flo11 là cần thiết cho sự phát triển xâm lấn và sự hình thành màng sinh học/mat. Flo11 thiếu glycosyl hóa không bị khiếm khuyết trong quá trình định vị bề mặt tế bào hoặc liên kết với các tế bào kiểu hoang dã trong quá trình chuyển đổi. Tuy nhiên, Flo11 kiểu hoang dã bị khiếm khuyết trong quá trình liên kết với bề mặt của các tế bào đang trải qua quá trình căng thẳng glycosyl hóa. Flo11 đã rụng và các glycoprotein khác đã rụng (Msb2 và Hkr1) cực kỳ ổn định với thời gian bán hủy theo thứ tự ngày. Glycosyl hóa của Flo11 góp phần vào sự ổn định của nó. Hơn nữa, sự cân bằng tổng thể giữa quá trình sản xuất Flo11, rụng và luân chuyển ủng hộ sự tích tụ của protein rụng theo thời gian. Những phát hiện của chúng tôi có thể áp dụng cho các phân tử bám dính nấm ở các loài khác bao gồm cả các tác nhân gây bệnh."} +{"idx": 1980103, "en": "• Soil aggregate stability is an important ecosystem property that is altered by anthropogenic disturbance. Yet, the generalization of these alterations and the identification of the main contributors are limited by the absence of cross-site comparisons and the application of inconsistent methodologies across regions. • We assessed aggregate stability in paired remnant and post-disturbance grasslands across California, shortgrass and tallgrass prairies, and in manipulative experiments of plant composition and soil microbial inoculation. • Grasslands recovering from anthropogenic disturbance consistently had lower aggregate stability than remnants. Across all grasslands, non-native plant diversity was significantly associated with reduced soil aggregate stability. A negative effect of non-native plants on aggregate stability was also observed in a mesocosm experiment comparing native and non-native plants from California grasslands. Moreover, an inoculation study demonstrated that the degradation of the microbial community also contributes to the decline in soil aggregate stability in disturbed grasslands. • Anthropogenic disturbance consistently reduced water-stable aggregates. The stability of aggregates was reduced by non-native plants and the degradation of the native soil microbial community. This latter effect might contribute to the sustained decline in aggregate stability following anthropogenic disturbance. Further exploration is advocated to understand the generality of these potential mechanisms.", "gemini": "• Độ ổn định của tổng hợp đất là một đặc tính quan trọng của hệ sinh thái bị thay đổi bởi sự xáo trộn do con người gây ra. Tuy nhiên, việc khái quát các thay đổi này và việc xác định những tác nhân chính gây ra chúng bị hạn chế bởi sự thiếu so sánh giữa các địa điểm và việc áp dụng các phương pháp không nhất quán giữa các vùng. • Chúng tôi đã đánh giá độ ổn định của tổng hợp trong các đồng cỏ còn sót lại và sau khi bị xáo trộn ở California, đồng cỏ ngắn và đồng cỏ cao, và trong các thí nghiệm thao tác về thành phần thực vật và tiêm chủng vi sinh vật đất. • Các đồng cỏ phục hồi sau sự xáo trộn do con người gây ra luôn có độ ổn định tổng hợp thấp hơn so với các đồng cỏ còn sót lại. Trong tất cả các đồng cỏ, sự đa dạng của các loài thực vật không phải bản địa có liên quan đáng kể đến việc giảm độ ổn định tổng hợp của đất. Tác động tiêu cực của các loài thực vật không phải bản địa đối với độ ổn định tổng hợp cũng được quan sát thấy trong một thí nghiệm mesocosm so sánh các loài thực vật bản địa và không phải bản địa từ các đồng cỏ ở California. Hơn nữa, một nghiên cứu tiêm chủng đã chứng minh rằng sự suy thoái của quần xã vi sinh vật cũng góp phần làm giảm độ ổn định tổng hợp của đất ở các đồng cỏ bị xáo trộn. • Sự xáo trộn do con người gây ra luôn làm giảm các tổng hợp ổn định với nước. Độ ổn định của các tổng hợp bị giảm do các loài thực vật không phải bản địa và sự suy thoái của quần xã vi sinh vật đất bản địa. Hiệu ứng sau này có thể góp phần vào sự suy giảm kéo dài độ ổn định tổng hợp sau sự xáo trộn do con người gây ra. Cần có thêm các cuộc khám phá để hiểu tính phổ biến của các cơ chế tiềm năng này."} +{"idx": 1980104, "en": "OBJECTIVE Depression and pain are often co-morbid. Temporomandibular disorders (TMD) include facial pain as one main symptom. Reports are lacking on the association between chronic facial pain and earlier depressiveness. The aim of the study was to investigate whether depressiveness increases the risk for chronic facial pain in a longitudinal population-based study. MATERIALS AND METHODS Subjects included in the Northern Finland Birth Cohort 1966 (n = 5696) answered a questionnaire on facial pain and depressiveness using the Symptom Checklist-25 depression sub-scale at the age of 31 years. In addition, reported depression diagnosed by a doctor was enquired about. Three years later a sub-sample of the cohort, including 63 cases with chronic facial pain and 85 pain-free controls, was formed based on the question concerning facial pain. RESULTS Of the chronic facial pain cases 17.5% and of the pain-free controls 7.1% were depressive 3 years earlier at baseline (p = 0.050, χ(2) test, crude OR = 2.8, 95% CI = 1.0-8.0). Of the chronic facial pain cases 6.3% and of the pain-free controls 1.2% reported having had diagnosed depression (p = 0.085, crude OR = 5.7, 95% CI = 0.6-52.2). After adjusting the gender, the association between depressiveness reported at the baseline and chronic facial pain was significant (OR = 4.2, 95% CI = 1.1-16.2). When widespread pain was included in the analysis, the association was not significant. CONCLUSIONS Depressiveness increases the risk for chronic facial pain in a 3-year follow-up. This association seems to be mediated through widespread pain.", "gemini": "MỤC TIÊU Trầm cảm và đau thường đi kèm với nhau. Rối loạn thái dương hàm (TMD) bao gồm đau mặt là một triệu chứng chính. Còn thiếu các báo cáo về mối liên quan giữa đau mặt mãn tính và tình trạng trầm cảm trước đó. Mục đích của nghiên cứu là tìm hiểu xem tình trạng trầm cảm có làm tăng nguy cơ đau mặt mãn tính trong một nghiên cứu theo dõi dựa trên dân số hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Các đối tượng tham gia Đội nghiên cứu sinh năm 1966 ở miền Bắc Phần Lan (n = 5696) đã trả lời một bảng câu hỏi về đau mặt và tình trạng trầm cảm bằng cách sử dụng thang đánh giá trầm cảm Symptom Checklist-25 khi họ 31 tuổi. Ngoài ra, còn có câu hỏi về tình trạng trầm cảm được bác sĩ chẩn đoán. Ba năm sau, một mẫu nhỏ của nhóm nghiên cứu, bao gồm 63 trường hợp đau mặt mãn tính và 85 đối chứng không đau, đã được hình thành dựa trên câu hỏi về đau mặt. KẾT QUẢ Trong số các trường hợp đau mặt mãn tính, 17,5% và trong số các đối chứng không đau, 7,1% bị trầm cảm 3 năm trước khi bắt đầu nghiên cứu (p = 0,050, kiểm tra χ(2), OR thô = 2,8, 95% CI = 1,0-8,0). Trong số các trường hợp đau mặt mãn tính, 6,3% và trong số các đối chứng không đau, 1,2% báo cáo đã được chẩn đoán mắc chứng trầm cảm (p = 0,085, OR thô = 5,7, 95% CI = 0,6-52,2). Sau khi điều chỉnh giới tính, mối liên quan giữa tình trạng trầm cảm được báo cáo khi bắt đầu nghiên cứu và đau mặt mãn tính là có ý nghĩa (OR = 4,2, 95% CI = 1,1-16,2). Khi đau lan rộng được đưa vào phân tích, mối liên quan này không có ý nghĩa. KẾT LUẬN Tình trạng trầm cảm làm tăng nguy cơ đau mặt mãn tính trong thời gian theo dõi 3 năm. Mối liên quan này dường như được trung gian thông qua cơn đau lan rộng."} +{"idx": 1980105, "en": "The authors describe here a unique case of contiguous, synchronous meningioma and lymphoma in the spinal column. Both tumors were present at the same vertebral level, one intradural and the other extradural. A patient presented with bilateral leg pain, acute weakness, and sensory loss in the lower extremities. Magnetic resonance imaging revealed an intradural mass at T6-7 with ambiguous boundaries relative to the thecal sac and compressing the spinal cord. The patient underwent resection of the epidural and intradural mass at T6-7. Histopathology revealed the epidural specimen to be a double-hit B-cell lymphoma and the intradural mass to be a transitional meningioma. Postoperatively, the patient did well, with an immediate return of strength and sensation. A postoperative MR image showed complete resection of the intradural mass. The authors suggest that biopsy may be prudent in patients with known systemic lymphoma presenting with a spinal lesion that has unclear boundaries relative to the thecal sac prior to commencing radiation and chemotherapy.", "gemini": "Các tác giả mô tả ở đây một trường hợp duy nhất về u màng não và u lympho đồng thời, tiếp giáp nhau ở cột sống. Cả hai khối u đều xuất hiện ở cùng một mức đốt sống, một khối ở trong màng cứng và khối kia ở ngoài màng cứng. Một bệnh nhân đến khám với triệu chứng đau hai chân, yếu cấp tính và mất cảm giác ở các chi dưới. Chụp cộng hưởng từ cho thấy một khối u trong màng cứng ở T6-7 với ranh giới mơ hồ so với bao màng cứng và chèn ép tủy sống. Bệnh nhân đã được cắt bỏ khối u ngoài màng cứng và trong màng cứng ở T6-7. Giải phẫu bệnh lý cho thấy mẫu vật ngoài màng cứng là u lympho tế bào B tấn công kép và khối u trong màng cứng là u màng não chuyển tiếp. Sau phẫu thuật, bệnh nhân tiến triển tốt, sức mạnh và cảm giác trở lại ngay lập tức. Hình ảnh chụp MR sau phẫu thuật cho thấy khối u trong màng cứng đã được cắt bỏ hoàn toàn. Các tác giả cho rằng sinh thiết có thể là thận trọng ở những bệnh nhân bị u lympho toàn thân đã biết biểu hiện bằng tổn thương cột sống có ranh giới không rõ ràng so với bao màng cứng trước khi bắt đầu xạ trị và hóa trị."} +{"idx": 1980106, "en": "BACKGROUND Fractionated technology has revolutionized laser therapy. With the success of initial devices, several fractionated lasers have appeared on the market. Claims of superiority have made device choice difficult for physicians and patients. MATERIALS AND METHODS Twelve subjects were treated with fractionated ablative lasers (10,600-nm carbon dioxide and 2790-nm yttrium scandium gallium garnet) in this institutional review board-approved trial. Each face was divided into four quadrants, and each quadrant was randomly treated using one of four lasers. Clinical experience was used to optimize settings. Two patients submitted biopsies from each quadrant immediately after treatment. Patients and blinded investigators assessed pain during treatment and post-treatment improvement in photoaging (measured by rhytides, lentigines, texture, and pore size) using a five-point scale. RESULTS All devices resulted in statistical improvement in photoaging in all patients, but no device was statistically significantly superior. No statistically significant difference was found in pain scores. All patients reported satisfaction 1 month after treatment. Three patients experienced adverse reactions. Histologically, there were no statistically significant differences between devices. CONCLUSIONS Fractionated ablative lasers reliably result in improvement in photoaging. Despite marketing claims, no statistically significant differences were found in outcomes, pain during treatment, or histologic findings. Even with experienced users, significant adverse reactions are possible.", "gemini": "BỐI CẢNH Công nghệ phân đoạn đã cách mạng hóa liệu pháp laser. Với sự thành công của các thiết bị ban đầu, một số laser phân đoạn đã xuất hiện trên thị trường. Các tuyên bố về tính ưu việt đã khiến việc lựa chọn thiết bị trở nên khó khăn đối với các bác sĩ và bệnh nhân. VẬT LIỆU VÀ PHƯƠNG PHÁP Mười hai đối tượng đã được điều trị bằng laser cắt bỏ phân đoạn (carbon dioxide 10.600 nm và granat yttrium scandium gallium 2790 nm) trong thử nghiệm đã được hội đồng đánh giá của tổ chức này chấp thuận. Mỗi khuôn mặt được chia thành bốn phần tư và mỗi phần tư được điều trị ngẫu nhiên bằng một trong bốn loại laser. Kinh nghiệm lâm sàng được sử dụng để tối ưu hóa cài đặt. Hai bệnh nhân đã nộp sinh thiết từ mỗi phần tư ngay sau khi điều trị. Bệnh nhân và các nhà điều tra mù đã đánh giá mức độ đau trong quá trình điều trị và cải thiện tình trạng lão hóa da sau điều trị (được đo bằng nếp nhăn, đốm đồi mồi, kết cấu và kích thước lỗ chân lông) bằng thang điểm năm điểm. KẾT QUẢ Tất cả các thiết bị đều dẫn đến sự cải thiện đáng kể về tình trạng lão hóa da ở tất cả bệnh nhân, nhưng không có thiết bị nào vượt trội hơn đáng kể về mặt thống kê. Không tìm thấy sự khác biệt có ý nghĩa thống kê về điểm đau. Tất cả bệnh nhân đều báo cáo hài lòng sau 1 tháng điều trị. Ba bệnh nhân gặp phản ứng có hại. Về mặt mô học, không có sự khác biệt có ý nghĩa thống kê giữa các thiết bị. KẾT LUẬN Laser cắt bỏ phân đoạn mang lại hiệu quả cải thiện tình trạng lão hóa da đáng tin cậy. Bất chấp các tuyên bố tiếp thị, không tìm thấy sự khác biệt có ý nghĩa thống kê về kết quả, mức độ đau trong quá trình điều trị hoặc các phát hiện mô học. Ngay cả với những người dùng có kinh nghiệm, vẫn có thể xảy ra các phản ứng có hại đáng kể."} +{"idx": 1980107, "en": "Polymeric delivery systems have been extensively studied to achieve localized and controlled release of protein drugs. However, it is still challenging to control the release of multiple protein drugs in distinct stages according to the progress of disease or treatment. This study successfully demonstrates that multiple protein drugs can be released from aptamer-functionalized hydrogels with adjustable release rates at predetermined time points using complementary sequences (CSs) as biomolecular triggers. Because both aptamer-protein interactions and aptamer-CS hybridization are sequence-specific, aptamer-functionalized hydrogels constitute a promising polymeric delivery system for the programmable release of multiple protein drugs to treat complex human diseases.", "gemini": "Các hệ thống phân phối polyme đã được nghiên cứu rộng rãi để đạt được sự giải phóng cục bộ và kiểm soát các loại thuốc protein. Tuy nhiên, việc kiểm soát sự giải phóng nhiều loại thuốc protein ở các giai đoạn riêng biệt theo tiến trình bệnh hoặc điều trị vẫn còn là một thách thức. Nghiên cứu này chứng minh thành công rằng nhiều loại thuốc protein có thể được giải phóng khỏi hydrogel chức năng hóa aptamer với tốc độ giải phóng có thể điều chỉnh tại các thời điểm đã định trước bằng cách sử dụng trình tự bổ sung (CS) làm chất kích hoạt sinh học. Vì cả tương tác aptamer-protein và lai hóa aptamer-CS đều là đặc hiệu theo trình tự, nên hydrogel chức năng hóa aptamer tạo thành một hệ thống phân phối polyme đầy hứa hẹn để giải phóng theo chương trình nhiều loại thuốc protein để điều trị các bệnh phức tạp ở người."} +{"idx": 1980108, "en": "CONTEXT Despite significant economic development in many parts of the world, upwards of 150 million children are short for their age. Epidemiologic and clinical data suggest that stunting increases the risk for nutrition-related chronic diseases (NRCD), a particular problem for transitional countries as dietary and environmental shifts that accompany development are also reported to increase the risk for NRCD. OBJECTIVE AND METHODS This paper reviews studies on the causes and long-term effects of poor nutrition as well as data from clinical studies of growth retardation and metabolism and body composition that may explain the link between poor growth and later risk for NRCD. RESULTS Growth retardation is the physical outcome of a complex interaction of socio- economic factors during key periods of development. Growth-retarded children may present with metabolic or body fat distribution profiles that predispose them to NRCD, a risk factor that may be compounded by consuming a diet consistent with the 'nutrition transition'. CONCLUSION It is important to continue studying the long-term physiological changes following recovery from under-nutrition, especially in countries undergoing a 'nutrition transition'. Estimates suggest that, within 20-30 years, ∼ 2/3 of the world population will reside in urban areas. The potential economic fallout of continued poor nutrition, poor growth and changing diets and activity patterns will be great given the healthcare costs and social problems associated with NRCD.", "gemini": "NGỮ CẢNH Mặc dù có sự phát triển kinh tế đáng kể ở nhiều nơi trên thế giới, nhưng vẫn có tới 150 triệu trẻ em bị thấp còi so với độ tuổi. Dữ liệu dịch tễ học và lâm sàng cho thấy tình trạng còi cọc làm tăng nguy cơ mắc các bệnh mãn tính liên quan đến dinh dưỡng (NRCD), một vấn đề đặc biệt đối với các quốc gia đang chuyển đổi vì chế độ ăn uống và những thay đổi về môi trường đi kèm với sự phát triển cũng được báo cáo là làm tăng nguy cơ mắc NRCD. MỤC TIÊU VÀ PHƯƠNG PHÁP Bài báo này xem xét các nghiên cứu về nguyên nhân và tác động lâu dài của tình trạng dinh dưỡng kém cũng như dữ liệu từ các nghiên cứu lâm sàng về tình trạng chậm phát triển và chuyển hóa và thành phần cơ thể có thể giải thích mối liên hệ giữa tình trạng tăng trưởng kém và nguy cơ mắc NRCD sau này. KẾT QUẢ Chậm phát triển là kết quả vật lý của sự tương tác phức tạp của các yếu tố kinh tế xã hội trong các giai đoạn phát triển quan trọng. Trẻ em chậm phát triển có thể biểu hiện với các đặc điểm phân bố chất béo trong cơ thể hoặc chuyển hóa khiến trẻ dễ mắc NRCD, một yếu tố nguy cơ có thể gia tăng do chế độ ăn uống phù hợp với 'chuyển đổi dinh dưỡng'. KẾT LUẬN Điều quan trọng là phải tiếp tục nghiên cứu những thay đổi sinh lý học lâu dài sau khi phục hồi tình trạng suy dinh dưỡng, đặc biệt là ở những quốc gia đang trải qua 'chuyển đổi dinh dưỡng'. Các ước tính cho thấy rằng, trong vòng 20-30 năm nữa, ∼ 2/3 dân số thế giới sẽ sinh sống ở khu vực đô thị. Hậu quả kinh tế tiềm ẩn của tình trạng dinh dưỡng kém kéo dài, tăng trưởng kém và chế độ ăn uống cũng như các kiểu hoạt động thay đổi sẽ rất lớn do chi phí chăm sóc sức khỏe và các vấn đề xã hội liên quan đến NRCD."} +{"idx": 1980109, "en": "The asymmetric allylic alkenylation of Morita-Baylis-Hillman (MBH) carbonates with N-itaconimides as nucleophiles has been developed using a commercially available Cinchona alkaloid catalyst. A variety of multifunctional chiral α-methylene-β-maleimide esters were attained in moderate to excellent yields (up to 99%) and good to excellent enantioselectivities (up to 91% ee). The origin of the regio- and stereoselectivity was verified by DFT methods. Calculated geometries and relative energies of various transition states strongly support the observed regio- and enantioselectivity.", "gemini": "Phản ứng alken hóa allylic không đối xứng của cacbonat Morita-Baylis-Hillman (MBH) với N-itaconimid làm chất ái nhân đã được phát triển bằng cách sử dụng chất xúc tác alkaloid Cinchona có sẵn trên thị trường. Nhiều este α-methylene-β-maleimide đa chức đã đạt được với hiệu suất từ trung bình đến tuyệt vời (lên đến 99%) và độ chọn lọc quang học từ tốt đến tuyệt vời (lên đến 91% ee). Nguồn gốc của tính chọn lọc vùng và tính chọn lọc lập thể đã được xác minh bằng phương pháp DFT. Các hình học được tính toán và năng lượng tương đối của các trạng thái chuyển tiếp khác nhau hỗ trợ mạnh mẽ cho tính chọn lọc vùng và tính chọn lọc lập thể đã quan sát được."} +{"idx": 1980110, "en": "The measurement of patients' satisfaction is increasingly becoming legal and ethical duty of healthcare providers worldwide. Till now, there were no validated, widely available Polish scales to assess patients' satisfaction with nursing care. The aim of this study was to assess the experiences and satisfaction with nursing care of patients hospitalized in surgical and nonsurgical wards in Poland using Polish version of the Newcastle Satisfaction with Nursing Scale. This is a multicentre, cross-sectional, descriptive study. Eight hospitals were qualified to participate in a convenience manner. The correlations between variables were analysed using the Mann-Whitney or the Kruskal-Wallis tests. In the case of a significant correlation, the Dunn's test was used to conduct multiple comparisons for the group of variables. A significance of α = 0.05 was assumed for the tests. A total 918 patients participated in the study, and 787 (85.72%) questionnaires were correctly filled out. The average result on the 'experience' scale was 73.22 (Me 73.07) and on the 'satisfaction' scale, 74.98 (Me 76.31). Education levels did not affect the experiences of nursing care levels p = 0.2204 and satisfaction with nursing care p = 0.1075. Patient age had a statistically significant impact on the results of the 'experiences of nursing care' scale p = 0.0005 and the 'satisfaction with nursing' scale p = 0.0194. The experiences of nursing care (p = 0.0002) and patients satisfaction (p = 0.0000) were significantly higher in surgical wards than in nonsurgical wards. The experiences of nursing care were significantly lower in the university hospital than in provincial hospitals (p = 0.0374) and district hospitals (p = 0.0183). A comparison of patient satisfaction with nursing in various hospitals shows that patients were most satisfied in district hospitals (average 78.10, Me 82.89), followed by provincial hospitals (average 72.11, Me 76.31) and the university hospital (average 70.64, Me 71.05).", "gemini": "Việc đo lường sự hài lòng của bệnh nhân ngày càng trở thành nghĩa vụ pháp lý và đạo đức của các nhà cung cấp dịch vụ chăm sóc sức khỏe trên toàn thế giới. Cho đến nay, vẫn chưa có thang điểm được xác thực, phổ biến rộng rãi ở Ba Lan để đánh giá mức độ hài lòng của bệnh nhân đối với dịch vụ chăm sóc điều dưỡng. Mục đích của nghiên cứu này là đánh giá kinh nghiệm và sự hài lòng đối với dịch vụ chăm sóc điều dưỡng của bệnh nhân nằm viện tại các khoa ngoại và nội khoa ở Ba Lan bằng cách sử dụng phiên bản tiếng Ba Lan của Thang đo sự hài lòng của Newcastle đối với dịch vụ chăm sóc điều dưỡng. Đây là một nghiên cứu mô tả, cắt ngang, đa trung tâm. Tám bệnh viện được đủ điều kiện để tham gia theo cách thuận tiện. Các mối tương quan giữa các biến được phân tích bằng các kiểm định Mann-Whitney hoặc Kruskal-Wallis. Trong trường hợp có mối tương quan đáng kể, kiểm định Dunn đã được sử dụng để thực hiện nhiều so sánh cho nhóm các biến. Một ý nghĩa của α = 0,05 được giả định cho các kiểm định. Tổng cộng có 918 bệnh nhân tham gia nghiên cứu và 787 (85,72%) bảng câu hỏi được điền chính xác. Kết quả trung bình trên thang đo 'kinh nghiệm' là 73,22 (Me 73,07) và trên thang đo 'hài lòng' là 74,98 (Me 76,31). Trình độ học vấn không ảnh hưởng đến kinh nghiệm về mức độ chăm sóc điều dưỡng p = 0,2204 và sự hài lòng đối với dịch vụ chăm sóc điều dưỡng p = 0,1075. Tuổi của bệnh nhân có tác động đáng kể về mặt thống kê đến kết quả của thang đo 'kinh nghiệm về dịch vụ chăm sóc điều dưỡng' p = 0,0005 và thang đo 'hài lòng đối với dịch vụ chăm sóc điều dưỡng' p = 0,0194. Kinh nghiệm về dịch vụ chăm sóc điều dưỡng (p = 0,0002) và sự hài lòng của bệnh nhân (p = 0,0000) cao hơn đáng kể ở các khoa ngoại trú so với các khoa nội trú. Kinh nghiệm về dịch vụ chăm sóc điều dưỡng thấp hơn đáng kể ở bệnh viện đại học so với các bệnh viện tỉnh (p = 0,0374) và bệnh viện huyện (p = 0,0183). So sánh sự hài lòng của bệnh nhân đối với dịch vụ chăm sóc điều dưỡng tại các bệnh viện khác nhau cho thấy bệnh nhân hài lòng nhất ở các bệnh viện huyện (trung bình 78,10, Me 82,89), tiếp theo là các bệnh viện tỉnh (trung bình 72,11, Me 76,31) và bệnh viện đại học (trung bình 70,64, Me 71,05)."} +{"idx": 1980111, "en": "In children, optimal timing of liver transplantation (LT) is crucial, but reliable prognostic tools for chronic liver diseases are scarce. We assessed the predictive value of galactose half-life (Gal½) for LT or death. A retrospective search of hospital database 2003-2010 revealed 92 consecutive children with chronic liver disease (36 biliary atresia) whose liver function was assessed with Gal½ measurement. Gal½, follow-up data, and liver biochemistry were recorded and pediatric/model for end-stage liver disease (P/MELD) scores calculated. Patients listed for LT or those who died within 1 year of the Gal½ measurement (Group 1) were compared to those surviving without listing (Group 2). Predictive value of Gal½ and P/MELD for listing for LT was assessed with area under the receiver operating characteristic curve (AUROC) analysis. Group 1 had markedly increased median Gal½ [17.0 (interquartile range 12.5-28.5) min] and higher P/MELD [13 (-1-23)] compared with group 2, [10.5 (9.5-12.5) min and -1 (-8-8); P < 0.001 for both]. Both Gal½ and P/MELD (P < 0.001) predicted listing or death with respective AUROCs of 0.808 (95% CI 0.704-0.913) and 0.780 (0.676-0.890), and 85% sensitivity and 69% specificity for Gal½≥12.0 min. Gal½ is a useful tool when evaluating 1-year prognosis in children with chronic liver disease.", "gemini": "Ở trẻ em, thời điểm tối ưu để ghép gan (LT) rất quan trọng, nhưng các công cụ tiên lượng đáng tin cậy cho các bệnh gan mạn lại rất khan hiếm. Chúng tôi đã đánh giá giá trị dự đoán của thời gian bán hủy galactose (Gal½) đối với LT hoặc tử vong. Việc tìm kiếm hồi cứu cơ sở dữ liệu bệnh viện từ năm 2003-2010 đã phát hiện ra 92 trẻ em liên tiếp mắc bệnh gan mạn (36 trẻ bị teo đường mật bẩm sinh) có chức năng gan được đánh giá bằng phép đo Gal½. Gal½, dữ liệu theo dõi và sinh hóa gan đã được ghi lại và điểm số bệnh gan giai đoạn cuối cho nhi khoa/mô hình (P/MELD) được tính toán. Những bệnh nhân được liệt kê để ghép LT hoặc những bệnh nhân tử vong trong vòng 1 năm kể từ khi đo Gal½ (Nhóm 1) được so sánh với những bệnh nhân sống sót mà không được liệt kê (Nhóm 2). Giá trị dự đoán của Gal½ và P/MELD đối với việc liệt kê ghép LT đã được đánh giá bằng diện tích dưới đường cong đặc trưng của người nhận (AUROC). Nhóm 1 có giá trị Gal½ trung bình tăng đáng kể [17,0 (khoảng cách giữa các tứ phân vị 12,5-28,5) phút] và P/MELD cao hơn [13 (-1-23)] so với nhóm 2, [10,5 (9,5-12,5) phút và -1 (-8-8); P < 0,001 cho cả hai]. Cả Gal½ và P/MELD (P < 0,001) đều dự đoán được việc liệt kê hoặc tử vong với AUROC tương ứng là 0,808 (95% CI 0,704-0,913) và 0,780 (0,676-0,890) và độ nhạy 85% và độ đặc hiệu 69% đối với Gal½≥12,0 phút. Gal½ là một công cụ hữu ích khi đánh giá tiên lượng 1 năm ở trẻ em mắc bệnh gan mạn."} +{"idx": 1980112, "en": "OBJECTIVE Hospital care for blacks is concentrated among a small number of hospitals and whether they have worse outcomes across common medical conditions is unknown. DATA SOURCE We used the 2007 100% Medicare file to calculate 30- and 90-day mortality rates for white and black patients admitted for acute myocardial infarction (AMI), congestive heart failure (CHF), or pneumonia. STUDY DESIGN We ranked all hospitals in the country by their proportion of discharged black patients and identified the top 10 percent of these hospitals as black serving. We examined race-specific adjusted mortality rates and adjusted for differences in hospital characteristics. PRINCIPAL FINDINGS At 30 days, black-serving hospitals had, compared with nonblack-serving hospitals, similar mortality for AMI, lower mortality for CHF, and higher mortality for pneumonia. At 90 days, mortality was higher at black-serving hospitals for both AMI and pneumonia and comparable for CHF compared with nonblack-serving hospitals. White patients had worse outcomes at black-serving hospitals for two conditions at 30 days and all three conditions at 90 days. Blacks also had worse outcomes at black-serving hospitals. CONCLUSIONS Hospitals with a high proportion of black patients had worse outcomes than other hospitals for both their white and black elderly patients.", "gemini": "MỤC TIÊU Việc chăm sóc bệnh nhân da đen tại bệnh viện tập trung ở một số ít bệnh viện và không rõ liệu họ có kết quả tệ hơn đối với các tình trạng bệnh lý phổ biến hay không. NGUỒN DỮ LIỆU Chúng tôi đã sử dụng hồ sơ Medicare 100% năm 2007 để tính tỷ lệ tử vong sau 30 và 90 ngày đối với bệnh nhân da trắng và da đen nhập viện vì nhồi máu cơ tim cấp (AMI), suy tim sung huyết (CHF) hoặc viêm phổi. THIẾT KẾ NGHIÊN CỨU Chúng tôi xếp hạng tất cả các bệnh viện trong cả nước theo tỷ lệ bệnh nhân da đen xuất viện và xác định 10 phần trăm bệnh viện hàng đầu trong số những bệnh viện này là bệnh viện phục vụ người da đen. Chúng tôi đã xem xét tỷ lệ tử vong được điều chỉnh theo chủng tộc và điều chỉnh theo sự khác biệt về đặc điểm của bệnh viện. KẾT LUẬN CHÍNH Ở thời điểm 30 ngày, các bệnh viện phục vụ người da đen có tỷ lệ tử vong tương tự đối với AMI, tỷ lệ tử vong thấp hơn đối với CHF và tỷ lệ tử vong cao hơn đối với viêm phổi so với các bệnh viện không phục vụ người da đen. Ở thời điểm 90 ngày, tỷ lệ tử vong cao hơn ở các bệnh viện phục vụ người da đen đối với cả AMI và viêm phổi và tương đương đối với CHF so với các bệnh viện không phục vụ người da đen. Bệnh nhân da trắng có kết quả tệ hơn tại các bệnh viện phục vụ người da đen đối với hai tình trạng bệnh lý ở thời điểm 30 ngày và cả ba tình trạng bệnh lý ở thời điểm 90 ngày. Người da đen cũng có kết quả tệ hơn tại các bệnh viện phục vụ người da đen. KẾT LUẬN Các bệnh viện có tỷ lệ bệnh nhân da đen cao có kết quả tệ hơn so với các bệnh viện khác đối với cả bệnh nhân da trắng và da đen lớn tuổi."} +{"idx": 1980113, "en": "Cyanobacteria act as primary producers of carbon and nitrogen in nutrient-poor ecosystems such as mangroves. This important group of microorganisms plays a critical role in sustaining the productivity of mangrove ecosystems, but the structure and function of cyanobacteria assemblages can be perturbed by anthropogenic influences. The aim of this work was to assess the community structure and ecological drivers that influence the cyanobacterial community harboured in two Brazilian mangrove soils, and examine the long-term effects of oil contamination on these keystone species. Community fingerprinting results showed that, although cyanobacterial communities are distinct between the two mangroves, the structure and diversity of the assemblages exhibit similar responses to environmental gradients. In each ecosystem, cyanobacteria occupying near-shore areas were similar in composition, indicating importance of marine influences for structuring the community. Analysis of 16S rRNA sequences revealed the presence of diverse cyanobacterial communities in mangrove sediments, with clear differences among mangrove habitats along a transect from shore to forest. While near-shore sites in both mangroves were mainly occupied by Prochlorococcus and Synechococcus genera, sequences retrieved from other mangrove niches were mainly affiliated with uncultured cyanobacterial 16S rRNA. The most intriguing finding was the large number of potentially novel cyanobacteria 16S rRNA sequences obtained from a previously oil-contaminated site. The abundance of cyanobacterial 16S rRNA sequences observed in sites with a history of oil contamination was significantly lower than in the unimpacted areas. This study emphasized the role of environmental drivers in determining the structure of cyanobacterial communities in mangrove soils, and suggests that anthropogenic impacts may also act as ecological filters that select cyanobacterial taxa. These results are an important contribution to our understanding of the composition and relative abundance of previously poorly described cyanobacterial assemblages in mangrove ecosystems.", "gemini": "Vi khuẩn lam đóng vai trò là sinh vật sản xuất chính về cacbon và nitơ trong các hệ sinh thái nghèo chất dinh dưỡng như rừng ngập mặn. Nhóm vi sinh vật quan trọng này đóng vai trò quan trọng trong việc duy trì năng suất của hệ sinh thái rừng ngập mặn, nhưng cấu trúc và chức năng của quần xã vi khuẩn lam có thể bị ảnh hưởng bởi tác động của con người. Mục đích của nghiên cứu này là đánh giá cấu trúc cộng đồng và các động lực sinh thái ảnh hưởng đến cộng đồng vi khuẩn lam trú ngụ trong hai loại đất rừng ngập mặn của Brazil và kiểm tra tác động lâu dài của ô nhiễm dầu đối với các loài then chốt này. Kết quả lấy dấu vân tay cộng đồng cho thấy, mặc dù các cộng đồng vi khuẩn lam khác biệt giữa hai rừng ngập mặn, nhưng cấu trúc và sự đa dạng của các quần xã này có phản ứng tương tự với các gradient môi trường. Trong mỗi hệ sinh thái, các vi khuẩn lam chiếm các khu vực gần bờ có thành phần tương tự, cho thấy tầm quan trọng của ảnh hưởng của biển đối với việc cấu trúc cộng đồng. Phân tích trình tự rRNA 16S cho thấy sự hiện diện của các cộng đồng vi khuẩn lam đa dạng trong trầm tích rừng ngập mặn, với sự khác biệt rõ ràng giữa các môi trường sống của rừng ngập mặn dọc theo một đường cắt ngang từ bờ đến rừng. Trong khi các địa điểm gần bờ ở cả hai rừng ngập mặn chủ yếu bị các chi Prochlorococcus và Synechococcus chiếm giữ, thì các trình tự thu được từ các hốc rừng ngập mặn khác chủ yếu liên kết với rRNA 16S của vi khuẩn lam chưa được nuôi cấy. Phát hiện thú vị nhất là số lượng lớn các trình tự rRNA 16S của vi khuẩn lam có khả năng mới thu được từ một địa điểm trước đây bị ô nhiễm dầu. Sự phong phú của các trình tự rRNA 16S của vi khuẩn lam được quan sát thấy ở các địa điểm có tiền sử ô nhiễm dầu thấp hơn đáng kể so với các khu vực không bị ảnh hưởng. Nghiên cứu này nhấn mạnh vai trò của các động lực môi trường trong việc xác định cấu trúc của các cộng đồng vi khuẩn lam trong đất rừng ngập mặn và cho thấy rằng tác động của con người cũng có thể đóng vai trò như các bộ lọc sinh thái chọn lọc các loài vi khuẩn lam. Những kết quả này là một đóng góp quan trọng vào sự hiểu biết của chúng ta về thành phần và độ phong phú tương đối của các quần xã vi khuẩn lam trước đây được mô tả kém trong hệ sinh thái rừng ngập mặn."} +{"idx": 1980114, "en": "BACKGROUND Delayed hypersensitivity (HS) reactions to potentiated sulfonamide antimicrobials occur in both dogs and humans, and involve an intermediate hydroxylamine metabolite that is detoxified by cytochrome b(5) and NADH cytochrome b(5) reductase. HYPOTHESIS/OBJECTIVES We hypothesized that polymorphisms in the genes (CYB5A and CYB5R3) encoding these 2 enzymes would be associated with risk of sulfonamide HS in dogs. ANIMALS A total of 18 dogs with delayed HS to potentiated sulfonamide antimicrobials and 16 dogs that tolerated (TOL) a therapeutic course of these drugs without adverse effect. METHODS CYB5A and CYB5R3 were sequenced from canine liver, and the promoter, exons, and 3' untranslated regions of both genes were resequenced from genomic DNA obtained from all dogs. RESULTS Multiple polymorphisms were found in both genes. When controlled for multiple comparisons, the 729GG variant in CYB5R3 was significantly overrepresented in dogs with sulfonamide HS (78% of dogs), compared to TOL dogs (31%; P = .003). CONCLUSIONS AND CLINICAL IMPORTANCE The CYB5R3 729GG variant may contribute to the risk of sulfonamide HS in dogs. Functional characterization of this polymorphism, as well as genotyping in a larger number of HS and TOL dogs, is warranted.", "gemini": "PHẢN ỨNG NHẠY CẢM CHẬM (HS) ĐỐI VỚI THUỐC KHÁNG KHUẨN SULFONAMID ĐƯỢC TĂNG CƯỜNG XẢY RA Ở CẢ CHÓ VÀ NGƯỜI, VÀ LIÊN QUAN ĐẾN CHẤT CHUYỂN HÓA TRUNG GIAN HYDROXYLAMINE ĐƯỢC CHUYỂN HÓA BỞI CYTOCHROME B(5) VÀ NADH CYTOCHROME B(5) REDUCTASE. GIẢ THUYẾT/MỤC TIÊU Chúng tôi giả thuyết rằng các đa hình trong các gen (CYB5A và CYB5R3) mã hóa 2 enzym này sẽ liên quan đến nguy cơ HS sulfonamid ở chó. ĐỘNG VẬT Tổng cộng có 18 con chó bị HS chậm với thuốc kháng khuẩn sulfonamid được tăng cường và 16 con chó dung nạp (TOL) một đợt điều trị bằng các loại thuốc này mà không có tác dụng phụ. PHƯƠNG PHÁP CYB5A và CYB5R3 được giải trình tự từ gan chó, và vùng khởi động, exon và vùng 3' không được dịch của cả hai gen được giải trình tự lại từ DNA bộ gen thu được từ tất cả các con chó. KẾT QUẢ Đã tìm thấy nhiều đa hình ở cả hai gen. Khi được kiểm soát để so sánh nhiều lần, biến thể 729GG trong CYB5R3 có tỷ lệ biểu hiện quá mức đáng kể ở những con chó bị HS sulfonamid (78% số chó), so với những con chó TOL (31%; P = 0,003). KẾT LUẬN VÀ TẦM QUAN TRỌNG LÂM SÀNG Biến thể CYB5R3 729GG có thể góp phần vào nguy cơ HS sulfonamid ở chó. Cần đặc tính hóa chức năng của đa hình này, cũng như tạo kiểu gen ở nhiều chó HS và TOL hơn."} +{"idx": 1980115, "en": "AIMS The ability of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing plant growth-promoting bacterial (PGPB) endophytes Pseudomonas fluorescens YsS6 and Pseudomonas migulae 8R6, their ACC deaminase minus mutants and the rhizospheric plant growth-promoting bacterium Pseudomonas putida UW4 to delay the senescence of mini carnation cut flowers was assessed. METHODS AND RESULTS Fresh cut flowers were incubated with either a bacterial cell suspension, the ethylene precursor ACC, the ethylene inhibitor l-α-(aminoethoxyvinyl)-glycine or 0·85% NaCl at room temperature for 11 days. Levels of flower senescence were recorded every other day. To verify the presence of endophytes inside the plant tissues, scanning electron microscopy was performed. Among all treatments, flowers treated with wild-type ACC deaminase-containing endophytic strains exhibited the most significant delay in flower senescence, while flowers treated with the ACC deaminase minus mutants senesced at a rate similar to the control. Flowers treated with Ps. putida UW4 senesced more rapidly than untreated control flowers. CONCLUSION The only difference between wild-type and mutant bacterial endophytes was ACC deaminase activity so that it may be concluded that this enzyme is directly responsible for the significant delay in flower senescence. Despite containing ACC deaminase activity, Ps. putida UW4 is not taken up by the cut flowers and therefore has no effect on prolonging their shelf life. SIGNIFICANCE AND IMPACT OF THE STUDY The world-wide cut flower industry currently uses expensive and potentially environmentally dangerous chemical inhibitors of ethylene to prolong the shelf life of cut flowers. The use of PGPB endophytes with ACC deaminase activity has the potential to replace the chemicals that are currently used by the cut flower industry.", "gemini": "MỤC TIÊU Khả năng của vi khuẩn nội sinh thúc đẩy sinh trưởng thực vật (PGPB) chứa 1-aminocyclopropan-1-carboxylate (ACC) deaminase Pseudomonas fluorescens YsS6 và Pseudomonas migulae 8R6, các đột biến âm tính ACC deaminase của chúng và vi khuẩn thúc đẩy sinh trưởng thực vật rễ Pseudomonas putida UW4 để trì hoãn quá trình lão hóa của hoa cẩm chướng mini đã được đánh giá. PHƯƠNG PHÁP VÀ KẾT QUẢ Hoa tươi cắt cành được ủ với huyền phù tế bào vi khuẩn, tiền chất ethylen ACC, chất ức chế ethylen l-α-(aminoethoxyvinyl)-glycine hoặc 0,85% NaCl ở nhiệt độ phòng trong 11 ngày. Mức độ lão hóa của hoa được ghi lại cách ngày. Để xác minh sự hiện diện của vi khuẩn nội sinh bên trong mô thực vật, đã tiến hành kính hiển vi điện tử quét. Trong số tất cả các phương pháp xử lý, những bông hoa được xử lý bằng các chủng vi khuẩn nội sinh chứa ACC deaminase kiểu hoang dã cho thấy sự chậm trễ đáng kể nhất trong quá trình lão hóa của hoa, trong khi những bông hoa được xử lý bằng các đột biến âm tính ACC deaminase bị lão hóa với tốc độ tương tự như nhóm đối chứng. Những bông hoa được xử lý bằng Ps. putida UW4 bị lão hóa nhanh hơn những bông hoa đối chứng chưa được xử lý. KẾT LUẬN Sự khác biệt duy nhất giữa vi khuẩn nội sinh kiểu hoang dã và đột biến là hoạt động ACC deaminase để có thể kết luận rằng enzym này chịu trách nhiệm trực tiếp cho sự chậm trễ đáng kể trong quá trình lão hóa của hoa. Mặc dù chứa hoạt động ACC deaminase, Ps. putida UW4 không được hoa cắt hấp thụ và do đó không có tác dụng kéo dài thời hạn sử dụng của chúng. Ý NGHĨA VÀ TÁC ĐỘNG CỦA NGHIÊN CỨU Ngành công nghiệp hoa cắt cành trên toàn thế giới hiện đang sử dụng các chất ức chế hóa học ethylen đắt tiền và có khả năng gây nguy hiểm cho môi trường để kéo dài thời hạn sử dụng của hoa cắt cành. Việc sử dụng vi khuẩn nội sinh PGPB có hoạt động ACC deaminase có khả năng thay thế các hóa chất hiện đang được ngành công nghiệp hoa cắt cành sử dụng."} +{"idx": 1980116, "en": "The management of myelodysplastic syndrome (MDS) remains challenging. We performed a phase I/II study to evaluate the safety and efficacy of decitabine in patients with MDS in Japan. Patients with MDS with red cell transfusion dependence or 5-30% blasts in marrow and with an International Prognostic Scoring System score of intermediate-1 or higher were eligible. Patients received intravenous decitabine at 15 or 20 mg/m(2) daily for 5 days every 4 weeks. A total of 37 patients were enrolled. Three patients received 15 mg/m(2) and experienced no dose limiting toxicity during the first cycle. Thirty-four patients received 20 mg/m(2) . Grade 3 or greater non-hematologic toxicities included cerebral infarction (n = 1), subdural hematoma (n = 1), elevated blood glucose (n = 1), and pulmonary hypertension (n = 1). At 20 mg/m(2) , complete response, partial response, and hematologic improvement were observed in 7 (20.6%), 2 (5.9%), and 7 (20.6%) patients, respectively. Complete cytogenetic response was observed in 30% of evaluable 20 patients. The median number of cycles to clinical response was 4 (range 4-8), and duration of remission was 474+ days (range 294-598+). The 2-year rate of acute myeloid leukemia-free survival was 52%. Correlative studies revealed hypomethylation in multiple genes in peripheral blood cells after treatment. Hypomethylation was generally more profound in CD15 + peripheral blood cells, which reflects myeloid cells, than in peripheral blood mononuclear cells. In summary, decitabine was safe and demonstrated efficacy in Japanese patients with high-risk MDS. This trial was registered at ClinicalTrials.gov (NCT00796003).", "gemini": "Việc quản lý hội chứng loạn sản tủy (MDS) vẫn còn nhiều thách thức. Chúng tôi đã thực hiện một nghiên cứu giai đoạn I/II để đánh giá tính an toàn và hiệu quả của decitabine ở những bệnh nhân mắc MDS tại Nhật Bản. Những bệnh nhân mắc MDS phụ thuộc truyền hồng cầu hoặc 5-30% tế bào nổ trong tủy và có điểm Hệ thống chấm điểm tiên lượng quốc tế từ trung gian-1 trở lên đủ điều kiện tham gia. Bệnh nhân được truyền tĩnh mạch decitabine với liều 15 hoặc 20 mg/m(2) hàng ngày trong 5 ngày cứ sau 4 tuần. Tổng cộng có 37 bệnh nhân được ghi danh. Ba bệnh nhân được dùng liều 15 mg/m(2) và không gặp độc tính giới hạn liều trong chu kỳ đầu tiên. Ba mươi bốn bệnh nhân được dùng liều 20 mg/m(2). Các độc tính không phải về huyết học ở mức độ 3 trở lên bao gồm nhồi máu não (n = 1), tụ máu dưới màng cứng (n = 1), tăng đường huyết (n = 1) và tăng huyết áp phổi (n = 1). Ở liều 20 mg/m(2), đáp ứng hoàn toàn, đáp ứng một phần và cải thiện huyết học lần lượt được quan sát thấy ở 7 (20,6%), 2 (5,9%) và 7 (20,6%) bệnh nhân. Đáp ứng tế bào di truyền hoàn toàn được quan sát thấy ở 30% trong số 20 bệnh nhân có thể đánh giá. Số chu kỳ trung bình để đáp ứng lâm sàng là 4 (khoảng 4-8) và thời gian thuyên giảm là 474+ ngày (khoảng 294-598+). Tỷ lệ sống không mắc bệnh bạch cầu cấp tính sau 2 năm là 52%. Các nghiên cứu tương quan cho thấy tình trạng giảm methyl hóa ở nhiều gen trong các tế bào máu ngoại vi sau khi điều trị. Giảm methyl hóa thường sâu hơn ở các tế bào máu ngoại vi CD15 +, phản ánh các tế bào tủy, so với các tế bào đơn nhân máu ngoại vi. Tóm lại, decitabine an toàn và đã chứng minh hiệu quả ở những bệnh nhân Nhật Bản mắc MDS có nguy cơ cao. Thử nghiệm này đã được đăng ký tại ClinicalTrials.gov (NCT00796003)."} +{"idx": 1980117, "en": "The cichlid fishes in the East African Great Lakes are a prime model system for the study of adaptive radiation. Therefore, the availability of an elaborate phylogenetic framework is an important prerequisite. Previous phylogenetic hypotheses on East African cichlids are mainly based on mitochondrial and/or fragment-based markers, and, to date, no taxon-rich phylogeny exists that is based on multilocus DNA sequence data. Here, we present the design of an extensive new primer set (24 nuclear makers) for East African cichlids that will be used for multilocus phylogenetic analyses in the future. The primers are designed to work for both Sanger sequencing and next-generation sequencing with the 454 technology. As a proof of principle, we validate these primers in a phylogenetically representative set of 16 cichlid species from Lake Tanganyika and main river systems in the area and provide a basic evaluation of the markers with respect to marker length and diversity indices.", "gemini": "Các loài cá rô phi ở Hồ lớn Đông Phi là một hệ thống mô hình chính để nghiên cứu bức xạ thích nghi. Do đó, việc có sẵn một khung hệ thống phát sinh loài chi tiết là một điều kiện tiên quyết quan trọng. Các giả thuyết về phát sinh loài trước đây trên các loài cá rô phi Đông Phi chủ yếu dựa trên các dấu hiệu dựa trên ty thể và/hoặc dựa trên đoạn, và cho đến nay, không có phát sinh loài nào giàu đơn vị phân loại dựa trên dữ liệu trình tự DNA đa gen. Ở đây, chúng tôi trình bày thiết kế của một bộ mồi mới mở rộng (24 nhà sản xuất hạt nhân) cho các loài cá rô phi Đông Phi sẽ được sử dụng cho các phân tích phát sinh loài đa gen trong tương lai. Các mồi được thiết kế để hoạt động cho cả giải trình tự Sanger và giải trình tự thế hệ tiếp theo với công nghệ 454. Để chứng minh nguyên lý, chúng tôi xác nhận các mồi này trong một tập hợp đại diện về mặt phát sinh loài gồm 16 loài cá rô phi từ Hồ Tanganyika và các hệ thống sông chính trong khu vực và cung cấp đánh giá cơ bản về các dấu hiệu liên quan đến chiều dài dấu hiệu và các chỉ số đa dạng."} +{"idx": 1980118, "en": "This article compares two national surveys carried out through the most commonly used procedures in Italy: CATI (computer-assisted telephone interviews) and SAQ-FI (self-answered questionnaires following interviews). Both surveys ask two identical questions concerning sensitive sexual behavior: early age at first intercourse and same-sex attraction. The SAQ-FI survey had both unit non-response and item non-response rates much lower than the CATI survey. Moreover, in the CATI survey, the groups with highest item non-response rates were also the groups with the lowest proportions of early intercourse and homosexual attraction. In addition, a differential analysis of the respondents produced diverse results for the two surveys. This is especially true of results by gender for same-sex attraction: Such behavior is more common among men (3.1%) than women (2.9%), according to the CATI survey, whereas the opposite is true of the SAQ-FI survey (6.1% of men vs. 7.7% women). In Italy at the beginning of the 21st century, CATI surveys reveal a lower level of early intercourse and same-sex attraction than SAQ-FI surveys. This article argues that the CATI survey underestimates the true level of these sensitive sexual behaviors in the Italian population.", "gemini": "Bài viết này so sánh hai cuộc khảo sát quốc gia được thực hiện thông qua các thủ tục được sử dụng phổ biến nhất ở Ý: CATI (phỏng vấn qua điện thoại hỗ trợ máy tính) và SAQ-FI (câu hỏi tự trả lời sau phỏng vấn). Cả hai cuộc khảo sát đều đặt hai câu hỏi giống hệt nhau liên quan đến hành vi tình dục nhạy cảm: tuổi đầu quan hệ tình dục và hấp dẫn đồng giới. Khảo sát SAQ-FI có tỷ lệ không trả lời đơn vị và tỷ lệ không trả lời mục thấp hơn nhiều so với khảo sát CATI. Hơn nữa, trong khảo sát CATI, các nhóm có tỷ lệ không trả lời mục cao nhất cũng là những nhóm có tỷ lệ quan hệ tình dục sớm và hấp dẫn đồng giới thấp nhất. Ngoài ra, một phân tích khác biệt về những người trả lời đã đưa ra những kết quả khác nhau cho hai cuộc khảo sát. Điều này đặc biệt đúng đối với kết quả theo giới tính đối với sự hấp dẫn đồng giới: Hành vi như vậy phổ biến hơn ở nam giới (3,1%) so với phụ nữ (2,9%), theo khảo sát CATI, trong khi điều ngược lại đúng với khảo sát SAQ-FI (6,1% nam giới so với 7,7% phụ nữ). Ở Ý vào đầu thế kỷ 21, các cuộc khảo sát CATI cho thấy mức độ quan hệ tình dục sớm và hấp dẫn đồng giới thấp hơn so với các cuộc khảo sát SAQ-FI. Bài viết này lập luận rằng cuộc khảo sát CATI đánh giá thấp mức độ thực sự của những hành vi tình dục nhạy cảm này trong dân số Ý."} +{"idx": 1980119, "en": "AIMS To study the antimicrobial resistance of coagulase-negative staphylococci (CoNS) in animals. METHODS AND RESULTS In the present study, a total of 87 CoNS recovered from food animals were characterized by antimicrobial susceptibility testing, resistance gene identification and conjugation. Of the seven species studied, Staphylococcus lentus, Staphylococcus sciuri, Staphylococcus xylosus and Staphylococcus haemolyticus accounted for over 96% of the isolates. In addition to β-lactam resistance (100%), high percentages of CoNS were resistant to tetracycline (67·8%), erythromycin (36·7%), clindamycin (27·5%) and quinopristin/dalfopristin (14·9%). Importantly, 47 (54%) isolates were resistant to at least three antimicrobial classes, including six CoNS resistant to six antimicrobial classes. The common genes for the above-mentioned resistance phenotypes were mec(A), tet(M), erm(A) and vga(A)(LC) , which were identified from 68·7%, 61%, 56·2% and 69·2% of the isolates, respectively. tet(M) was conjugatively transferable from 10 tetracycline-resistant CoNS to a Enterococcus strain, underlining the potential of antimicrobial resistance transfer from Staphylococcus to the commensal bacteria in human. CONCLUSIONS Multidrug resistance and resistance to non-β-lactam antimicrobials are common in CoNS in animals. SIGNIFICANCE AND IMPACT OF THE STUDY The data improve our understanding on the extent to which CoNS contribute to the dissemination of antimicrobial resistance in the food production environment.", "gemini": "MỤC ĐÍCH Nghiên cứu khả năng kháng thuốc của tụ cầu âm tính với coagulase (CoNS) ở động vật. PHƯƠNG PHÁP VÀ KẾT QUẢ Trong nghiên cứu hiện tại, tổng cộng 87 CoNS thu được từ động vật thực phẩm đã được đặc trưng bằng xét nghiệm nhạy cảm với thuốc kháng khuẩn, xác định gen kháng thuốc và liên hợp. Trong số bảy loài được nghiên cứu, Staphylococcus lentus, Staphylococcus sciuri, Staphylococcus xylosus và Staphylococcus haemolyticus chiếm hơn 96% các phân lập. Ngoài khả năng kháng β-lactam (100%), tỷ lệ phần trăm cao CoNS đã kháng tetracycline (67,8%), erythromycin (36,7%), clindamycin (27,5%) và quinopristin/dalfopristin (14,9%). Điều quan trọng là 47 (54%) phân lập đã kháng ít nhất ba loại thuốc kháng khuẩn, bao gồm sáu CoNS kháng sáu loại thuốc kháng khuẩn. Các gen phổ biến cho các kiểu hình kháng thuốc đã đề cập ở trên là mec(A), tet(M), erm(A) và vga(A)(LC), được xác định từ 68,7%, 61%, 56,2% và 69,2% các phân lập, tương ứng. tet(M) có thể liên hợp từ 10 CoNS kháng tetracycline sang một chủng Enterococcus, làm nổi bật khả năng chuyển gen kháng thuốc từ Staphylococcus sang vi khuẩn cộng sinh ở người. KẾT LUẬN Kháng thuốc đa và kháng thuốc kháng khuẩn không phải β-lactam phổ biến ở CoNS ở động vật. Ý NGHĨA VÀ TÁC ĐỘNG CỦA NGHIÊN CỨU Các dữ liệu cải thiện sự hiểu biết của chúng tôi về mức độ mà CoNS góp phần vào việc phát tán khả năng kháng thuốc trong môi trường sản xuất thực phẩm."} +{"idx": 1980120, "en": "BACKGROUND Previous work indicated that psychosocial factors (depression and somatization) are more strongly associated with symptom severity and weight loss in functional dyspepsia (FD) than gastric sensorimotor function. However, there is conflicting evidence regarding the association of these etiopathogenetic factors with Rome III symptom-based subgroups in FD [epigastric pain syndrome (EPS), postprandial distress syndrome (PDS)]. We aimed to test whether gastric sensitivity and emptying, depression, and somatization are differentially associated with empirically derived functional gastroduodenal disorders (FGD) symptom factors in one comprehensive model. METHODS In 259 tertiary care FD patients, we studied gastric sensorimotor function with barostat and gastric emptying breath test. Depression, somatization, and FGD symptoms were measured using self-report questionnaires. Confirmatory factor analysis (CFA) on 7 FGD symptoms was used to determine the fit of a latent variable structure based on Rome III symptom-based subgroups. Structural equation modeling (SEM) was used to test the putative relationships of the symptom factors with gastric sensorimotor function, depression, and somatization. KEY RESULTS The results of the CFA show a good fit [C(min) /DF = 1.54, CFI(comparative fit index) = 0.97] for the three-factor solution based on Rome III subgroups. The SEM also fitted the data well (C(min) /DF = 1.24, CFI = 0.98) and demonstrated that gastric sensitivity and depression are associated with PDS and nausea and vomiting. Gastric emptying is uniquely associated with EPS and somatization is strongly associated with all three symptom factors. CONCLUSIONS & INFERENCES Confirmatory factor analysis confirms the existence of three FGD symptom factors, corresponding to Rome III symptom-based subgroups. The SEM results suggest that different psychobiological mechanisms may play a role in these subgroups.", "gemini": "BỐI CẢNH Các công trình trước đây chỉ ra rằng các yếu tố tâm lý xã hội (trầm cảm và biểu hiện cơ thể) có liên quan chặt chẽ hơn với mức độ nghiêm trọng của triệu chứng và sụt cân trong chứng khó tiêu chức năng (FD) so với chức năng cảm giác vận động dạ dày. Tuy nhiên, có bằng chứng trái ngược nhau về mối liên quan của các yếu tố bệnh sinh này với các phân nhóm dựa trên triệu chứng Rome III trong FD [hội chứng đau thượng vị (EPS), hội chứng đau bụng sau ăn (PDS)]. Chúng tôi muốn kiểm tra xem độ nhạy và làm rỗng dạ dày, trầm cảm và biểu hiện cơ thể có liên quan khác nhau với các yếu tố triệu chứng rối loạn chức năng dạ dày tá tràng (FGD) thu được theo kinh nghiệm trong một mô hình toàn diện hay không. PHƯƠNG PHÁP Đối với 259 bệnh nhân FD được chăm sóc tại tuyến ba, chúng tôi đã nghiên cứu chức năng cảm giác vận động dạ dày bằng máy đo áp lực và xét nghiệm thở làm rỗng dạ dày. Trầm cảm, biểu hiện cơ thể và các triệu chứng FGD được đo bằng bảng câu hỏi tự báo cáo. Phân tích nhân tố xác nhận (CFA) trên 7 triệu chứng FGD được sử dụng để xác định sự phù hợp của cấu trúc biến tiềm ẩn dựa trên các phân nhóm dựa trên triệu chứng Rome III. Mô hình phương trình cấu trúc (SEM) được sử dụng để kiểm tra các mối quan hệ giả định của các yếu tố triệu chứng với chức năng cảm giác vận động dạ dày, trầm cảm và biểu hiện cơ thể. KẾT QUẢ CHÍNH Kết quả của CFA cho thấy sự phù hợp tốt [C(min) / DF = 1,54, CFI (chỉ số phù hợp so sánh) = 0,97] đối với giải pháp ba nhân tố dựa trên các phân nhóm Rome III. SEM cũng phù hợp tốt với dữ liệu (C(min) / DF = 1,24, CFI = 0,98) và chứng minh rằng độ nhạy của dạ dày và trầm cảm có liên quan đến PDS và buồn nôn và nôn. Làm rỗng dạ dày có liên quan duy nhất với EPS và biểu hiện cơ thể có liên quan chặt chẽ với cả ba yếu tố triệu chứng. KẾT LUẬN & ĐỀ XUẤT Phân tích nhân tố xác nhận khẳng định sự tồn tại của ba yếu tố triệu chứng FGD, tương ứng với các phân nhóm dựa trên triệu chứng Rome III. Kết quả SEM cho thấy rằng các cơ chế tâm sinh học khác nhau có thể đóng vai trò trong các phân nhóm này."} +{"idx": 1980121, "en": "OBJECTIVE To synthesize evidence on the accuracy of Medicaid reporting across state and federal surveys. DATA SOURCES All available validation studies. STUDY DESIGN Compare results from existing research to understand variation in reporting across surveys. DATA COLLECTION METHODS Synthesize all available studies validating survey reports of Medicaid coverage. PRINCIPAL FINDINGS Across all surveys, reporting some type of insurance coverage is better than reporting Medicaid specifically. Therefore, estimates of uninsurance are less biased than estimates of specific sources of coverage. The CPS stands out as being particularly inaccurate. CONCLUSIONS Measuring health insurance coverage is prone to some level of error, yet survey overstatements of uninsurance are modest in most surveys. Accounting for all forms of bias is complex. Researchers should consider adjusting estimates of Medicaid and uninsurance in surveys prone to high levels of misreporting.", "gemini": "MỤC TIÊU Tổng hợp bằng chứng về tính chính xác của báo cáo Medicaid trên các cuộc khảo sát của tiểu bang và liên bang. NGUỒN DỮ LIỆU Tất cả các nghiên cứu xác nhận có sẵn. THIẾT KẾ NGHIÊN CỨU So sánh kết quả từ các nghiên cứu hiện có để hiểu sự khác biệt trong báo cáo trên các cuộc khảo sát. PHƯƠNG PHÁP THU THẬP DỮ LIỆU Tổng hợp tất cả các nghiên cứu có sẵn xác nhận các báo cáo khảo sát về phạm vi bảo hiểm Medicaid. KẾT LUẬN CHÍNH Trên tất cả các cuộc khảo sát, việc báo cáo một số loại bảo hiểm tốt hơn so với báo cáo cụ thể về Medicaid. Do đó, ước tính về tình trạng không có bảo hiểm ít bị sai lệch hơn so với ước tính về các nguồn bảo hiểm cụ thể. CPS nổi bật vì đặc biệt không chính xác. KẾT LUẬN Đo lường phạm vi bảo hiểm y tế dễ xảy ra một số lỗi, nhưng việc báo cáo quá mức về tình trạng không có bảo hiểm trong hầu hết các cuộc khảo sát là khiêm tốn. Việc giải thích cho tất cả các hình thức thiên vị là phức tạp. Các nhà nghiên cứu nên xem xét việc điều chỉnh các ước tính về Medicaid và tình trạng không có bảo hiểm trong các cuộc khảo sát dễ xảy ra tình trạng báo cáo sai cao."} +{"idx": 1980122, "en": "Despite the possible occurrence of metal nanoparticles in the environment due to the discharge of engineered nanoparticles and the natural transformation of metal ions into metal nanoparticles, little is known about the transformation mechanism, fates, behaviors, and effects of these nanoparticles in the environment. Here, we show that dissolved organic matter (DOM) in environmental waters can mediate the reduction of ionic Ag and Au to their metallic nanoparticles under natural sunlight, suggesting that this process may be general for metals with high reduction potential. We demonstrated that the reduction was mediated by superoxide from photoirradiation of the phenol group in DOM, and the dissolved O(2) significantly enhanced the formation of Ag nanoparticles. These results imply that previous knowledge about O(2)-induced dissolution and its effect on persistence of Ag nanoparticles should be reconsidered in a sunlit DOM-rich aqueous environment. This study can also shed light on understanding possible natural sources of Ag and Au nanoparticles in the aquatic environment, which is possibly critical in the supergene enrichment of Ag and Au.", "gemini": "Mặc dù có thể xảy ra các hạt nano kim loại trong môi trường do sự giải phóng các hạt nano được thiết kế và sự chuyển đổi tự nhiên của các ion kim loại thành các hạt nano kim loại, nhưng người ta vẫn biết rất ít về cơ chế chuyển đổi, số phận, hành vi và tác động của các hạt nano này trong môi trường. Ở đây, chúng tôi chỉ ra rằng vật chất hữu cơ hòa tan (DOM) trong nước môi trường có thể trung gian quá trình khử Ag và Au ion thành các hạt nano kim loại của chúng dưới ánh sáng mặt trời tự nhiên, cho thấy rằng quá trình này có thể phổ biến đối với các kim loại có điện thế khử cao. Chúng tôi chứng minh rằng quá trình khử được trung gian bởi siêu oxit từ quá trình chiếu sáng phenol trong DOM và O(2) hòa tan làm tăng đáng kể sự hình thành các hạt nano Ag. Những kết quả này ngụ ý rằng kiến thức trước đây về quá trình hòa tan do O(2) gây ra và tác động của nó đối với sự tồn tại của các hạt nano Ag nên được xem xét lại trong môi trường nước giàu DOM có ánh sáng mặt trời. Nghiên cứu này cũng có thể làm sáng tỏ việc hiểu các nguồn tự nhiên có thể có của các hạt nano Ag và Au trong môi trường nước, có thể rất quan trọng trong quá trình làm giàu siêu gen của Ag và Au."} +{"idx": 1980123, "en": "AIM To evaluate the influence of adhesive point dimension and splint type on the rigidity of wire-composite splints in vitro. MATERIALS AND METHODS A custom-made artificial model was used. The two central incisors served as injured teeth (degrees of loosening III and II) and the two lateral incisors as non-injured teeth (physiological mobility). Horizontal and vertical tooth mobilities were investigated before and after splinting with the Periotest(®) method; the percent change was taken as the relative splint effect. Teeth were splinted with three types of wire-composite splints: Dentaflex (0.45 mm), Strengtheners (0.8 × 1.8 mm), and Dentaflex completely covered with composite. Four adhesive point dimensions (2, 3, 4, and 5 mm) were evaluated. Normal distribution was tested with the Kolmogorov-Smirnov test. Differences were evaluated with the anova and post hoc tests for pair-wise comparisons. Significance level was set at 0.05. RESULTS The adhesive point dimension did not influence splint rigidity, in general ( P  = 0.288). Significant effects were found in non-injured teeth with the Dentaflex ( P  < 0.001) and in injured teeth with the Strengtheners ( P  < 0.001). The Strengtheners splint rigidity increased significantly with increasing adhesive point dimensions. The three splints showed significantly different effects at 5-mm adhesive point dimension ( P  < 0.001). CONCLUSION Splint rigidity for injured teeth was influenced by adhesive point dimension only when splinting with Strengtheners. We recommend adapting splint rigidity by selecting different wires and reducing the adhesive point dimension to a minimum. Dentaflex can be used for flexible splinting, Strengtheners, and composite covered Dentaflex for rigid splinting.", "gemini": "MỤC ĐÍCH Đánh giá ảnh hưởng của kích thước điểm kết dính và loại máng nhai đến độ cứng của máng nhai dây-composite trong ống nghiệm. VẬT LIỆU VÀ PHƯƠNG PHÁP Một mô hình nhân tạo được làm riêng đã được sử dụng. Hai răng cửa giữa đóng vai trò là răng bị tổn thương (mức độ lung lay III và II) và hai răng cửa bên đóng vai trò là răng không bị tổn thương (khả năng vận động sinh lý). Độ vận động của răng theo chiều ngang và chiều dọc đã được nghiên cứu trước và sau khi nẹp bằng phương pháp Periotest(®); thay đổi phần trăm được coi là hiệu ứng nẹp tương đối. Răng được nẹp bằng ba loại máng nhai dây-composite: Dentaflex (0,45 mm), Strengtheners (0,8 × 1,8 mm) và Dentaflex được phủ hoàn toàn bằng composite. Bốn kích thước điểm kết dính (2, 3, 4 và 5 mm) đã được đánh giá. Phân phối chuẩn được kiểm tra bằng kiểm tra Kolmogorov-Smirnov. Sự khác biệt được đánh giá bằng các bài kiểm tra anova và kiểm định sau khi kiểm tra từng cặp. Mức ý nghĩa được đặt ở mức 0,05. KẾT QUẢ Kích thước điểm kết dính không ảnh hưởng đến độ cứng của máng nhai, nói chung (P = 0,288). Tác động đáng kể được tìm thấy ở răng không bị tổn thương với Dentaflex (P < 0,001) và ở răng bị tổn thương với Strengtheners (P < 0,001). Độ cứng của máng nhai Strengtheners tăng đáng kể khi kích thước điểm kết dính tăng. Ba máng nhai cho thấy tác động khác nhau đáng kể ở kích thước điểm kết dính 5 mm (P < 0,001). KẾT LUẬN Độ cứng của máng nhai đối với răng bị tổn thương chỉ bị ảnh hưởng bởi kích thước điểm kết dính khi nẹp bằng Strengtheners. Chúng tôi khuyên bạn nên điều chỉnh độ cứng của máng nhai bằng cách chọn các loại dây khác nhau và giảm kích thước điểm kết dính xuống mức tối thiểu. Dentaflex có thể được sử dụng để nẹp linh hoạt, Strengtheners và Dentaflex phủ composite để nẹp cứng."} +{"idx": 1980124, "en": "• To our knowledge, to date, no nonempirical method exists to measure reverse, low or high sap flux density. Moreover, existing sap flow methods require destructive wood core measurements to determine sapwood water content, necessary to convert heat velocity to sap flux density, not only damaging the tree, but also neglecting seasonal variability in sapwood water content. • Here, we present a nonempirical heat-pulse-based method and coupled sensor which measure temperature changes around a linear heater in both axial and tangential directions after application of a heat pulse. By fitting the correct heat conduction-convection equation to the measured temperature profiles, the heat velocity and water content of the sapwood can be determined. • An identifiability analysis and validation tests on artificial and real stem segments of European beech (Fagus sylvatica L.) confirm the applicability of the method, leading to accurate determinations of heat velocity, water content and hence sap flux density. • The proposed method enables sap flux density measurements to be made across the entire natural occurring sap flux density range of woody plants. Moreover, the water content during low flows can be determined accurately, enabling a correct conversion from heat velocity to sap flux density without destructive core measurements.", "gemini": "• Theo hiểu biết của chúng tôi, cho đến nay, không có phương pháp không kinh nghiệm nào để đo mật độ dòng nhựa ngược, thấp hoặc cao. Hơn nữa, các phương pháp dòng nhựa hiện có yêu cầu đo lõi gỗ phá hủy để xác định hàm lượng nước của gỗ dác, cần thiết để chuyển đổi vận tốc nhiệt thành mật độ dòng nhựa, không chỉ làm hỏng cây mà còn bỏ qua sự thay đổi theo mùa trong hàm lượng nước của gỗ dác. • Tại đây, chúng tôi trình bày một phương pháp dựa trên xung nhiệt không kinh nghiệm và cảm biến kết hợp đo những thay đổi nhiệt độ xung quanh một bộ phận gia nhiệt tuyến tính theo cả hướng trục và hướng tiếp tuyến sau khi áp dụng xung nhiệt. Bằng cách kết hợp phương trình dẫn nhiệt-đối lưu chính xác với các hồ sơ nhiệt độ đã đo, có thể xác định được vận tốc nhiệt và hàm lượng nước của gỗ dác. • Một phân tích khả năng nhận dạng và các bài kiểm tra xác thực trên các đoạn thân cây nhân tạo và thực của cây sồi châu Âu (Fagus sylvatica L.) xác nhận khả năng áp dụng của phương pháp, dẫn đến việc xác định chính xác vận tốc nhiệt, hàm lượng nước và do đó là mật độ dòng nhựa. • Phương pháp được đề xuất cho phép đo mật độ dòng nhựa trong toàn bộ phạm vi mật độ dòng nhựa tự nhiên của cây thân gỗ. Hơn nữa, có thể xác định chính xác hàm lượng nước trong quá trình dòng chảy thấp, cho phép chuyển đổi chính xác từ vận tốc nhiệt thành mật độ dòng nhựa mà không cần đo lõi phá hủy."} +{"idx": 1980125, "en": "BACKGROUND Kangaroo Mother Care (KMC) supports parents' role at the neonatal intensive care unit (NICU). To enhance parents' provision of KMC, it is essential to obtain knowledge of what parents perceive as supportive factors and barriers regarding their opportunities to perform KMC. AIM To identify factors that parents of preterm infants perceived as supportive factors or barriers for their performance of KMC and to explore the timing of and reasons for parents' discontinuation of KMC. METHODS A descriptive study performed at two NICUs in Sweden with 76 mothers and 74 fathers of preterm infants born at gestational ages ranging from 28 to 33 weeks. Data on infant characteristics were obtained from the infants' medical records. A questionnaire, based on scientific literature and the researchers' clinical experience, was completed by the mothers and the fathers separately, shortly after the infant's discharge from the hospital. The data were analyzed with qualitative content analysis and descriptive statistic. RESULTS Four categories were identified in parents' responses regarding support and barriers for their performance of KMC: Parent related factors, Time, Infants related factors and The NICU and home environment. The hospital staff and environment were described by the parents as both supportive and barriers for their application of KMC. Some mothers described the infants' feeding process as an obstacle to KMC. Sleeping with the infant skin-to-skin in the same position throughout the night could be difficult, as an uncomfortable sleeping position caused insufficient sleep. A majority of both mothers and fathers continued providing their infant with KMC to some extent after discharge. CONCLUSION Interventions for enhancing parents' opportunities for performing KMC should address both hospital staff attitudes and practices and the NICU environment.", "gemini": "LÝ LỊCH Chăm sóc mẹ Kangaroo (KMC) hỗ trợ vai trò của cha mẹ tại khoa chăm sóc tích cực sơ sinh (NICU). Để nâng cao khả năng cung cấp KMC của cha mẹ, điều cần thiết là phải có kiến thức về những gì cha mẹ coi là các yếu tố hỗ trợ và rào cản liên quan đến cơ hội thực hiện KMC của họ. MỤC ĐÍCH Xác định các yếu tố mà cha mẹ của trẻ sinh non coi là yếu tố hỗ trợ hoặc rào cản đối với việc thực hiện KMC và khám phá thời điểm và lý do khiến cha mẹ ngừng KMC. PHƯƠNG PHÁP Một nghiên cứu mô tả được thực hiện tại hai NICU ở Thụy Điển với 76 bà mẹ và 74 ông bố của trẻ sinh non được sinh ra ở tuổi thai từ 28 đến 33 tuần. Dữ liệu về đặc điểm của trẻ sơ sinh được lấy từ hồ sơ bệnh án của trẻ. Một bảng câu hỏi dựa trên các tài liệu khoa học và kinh nghiệm lâm sàng của các nhà nghiên cứu đã được các bà mẹ và các ông bố hoàn thành riêng biệt, ngay sau khi trẻ sơ sinh xuất viện. Dữ liệu được phân tích bằng phương pháp phân tích nội dung định tính và thống kê mô tả. KẾT QUẢ Bốn loại đã được xác định trong phản hồi của cha mẹ liên quan đến sự hỗ trợ và rào cản đối với việc thực hiện KMC của họ: Các yếu tố liên quan đến cha mẹ, Thời gian, Các yếu tố liên quan đến trẻ sơ sinh và Môi trường NICU và gia đình. Các bậc cha mẹ mô tả nhân viên và môi trường bệnh viện vừa là sự hỗ trợ vừa là rào cản đối với việc áp dụng KMC của họ. Một số bà mẹ mô tả quá trình cho trẻ sơ sinh bú như một trở ngại đối với KMC. Ngủ với trẻ sơ sinh da kề da ở cùng một tư thế trong suốt đêm có thể khó khăn, vì tư thế ngủ không thoải mái khiến trẻ không ngủ đủ giấc. Phần lớn các bà mẹ và các ông bố vẫn tiếp tục cung cấp KMC cho trẻ sơ sinh ở một mức độ nào đó sau khi xuất viện. KẾT LUẬN Các biện pháp can thiệp nhằm nâng cao cơ hội thực hiện KMC của cha mẹ nên giải quyết cả thái độ và hành vi của nhân viên bệnh viện và môi trường NICU."} +{"idx": 1980126, "en": "Amalgam has been used in the restoration of structurally compromised posterior teeth for many years. When placing large amalgam restorations, replacement of weak cusps with restorative material is recommended to prevent tooth fracture. This recommendation can be modified with new guidelines using modern adhesive techniques. Semidirect and indirect inlay/onlay composite restorations have progressively replaced amalgam restorations over the past 20 years. Lately, single visit direct resin-bonded composite (RBC) restorations have also been used as a viable alternative to conventional indirect restorations. This paper is intended to introduce a step-by-step protocol for the direct restoration of structurally compromised posterior teeth using RBCs with stress-reducing protocols.", "gemini": "Amalgam đã được sử dụng trong phục hồi răng hàm sau bị tổn thương về mặt cấu trúc trong nhiều năm. Khi đặt phục hồi amalgam lớn, khuyến cáo thay thế các mấu yếu bằng vật liệu phục hồi để ngăn ngừa gãy răng. Khuyến cáo này có thể được sửa đổi bằng các hướng dẫn mới sử dụng các kỹ thuật kết dính hiện đại. Phục hồi composite khảm/lớp phủ bán trực tiếp và gián tiếp đã dần thay thế phục hồi amalgam trong 20 năm qua. Gần đây, phục hồi composite liên kết nhựa trực tiếp một lần cũng đã được sử dụng như một giải pháp thay thế khả thi cho phục hồi gián tiếp thông thường. Bài báo này nhằm giới thiệu một giao thức từng bước để phục hồi trực tiếp răng hàm sau bị tổn thương về mặt cấu trúc bằng RBC với các giao thức giảm ứng suất."} +{"idx": 1980127, "en": "BACKGROUND AND PURPOSE To evaluate a dipstick algorithm for urinary tract colonization, prior to high-dose corticosteroid treatment in acute relapses of multiple sclerosis (MS). METHODS Prospective cohort study of 267 consecutive patients with MS relapses requiring corticosteroid treatment in a hospital-based, ambulatory, acute MS relapse clinic. A total of 18 participants met the exclusion criteria, leaving 249 for analysis. Main outcome measures were urinary dipstick sensitivity, specificity, positive predictive value, negative predictive value and safety of antibiotic co-treatment with high-dose corticosteroids. RESULTS Significant bacteriuria (≥10(5) colonies ml) rate in this population was 11% (95% CI, 7.1-14.9). Specificity and sensitivity of positive leucocyte esterase or nitrite were 78% and 65%. Negative predictive value of urine dipstick was 96%. No clinical adverse events occurred in the 3% (95% CI, 0.9-5.1) of patients with a false-negative dipstick. Eighteen per cent of patients were unnecessarily treated with antibiotics for 48 h. CONCLUSION Urinary dipstick testing allows for rapid and safe management of patients suffering from an acute MS relapse. The algorithm is conservative, and future work is needed to reduce the false-positive rate.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Đánh giá thuật toán que thử đối với tình trạng nhiễm trùng đường tiết niệu, trước khi điều trị corticosteroid liều cao trong các đợt tái phát cấp tính của bệnh đa xơ cứng (MS). PHƯƠNG PHÁP Nghiên cứu theo dõi tiến triển của 267 bệnh nhân liên tiếp bị tái phát MS cần điều trị corticosteroid tại phòng khám ngoại trú, cấp tính, tái phát MS tại bệnh viện. Tổng cộng có 18 người tham gia đáp ứng tiêu chuẩn loại trừ, còn lại 249 người được phân tích. Các biện pháp kết quả chính là độ nhạy, độ đặc hiệu, giá trị dự đoán dương tính, giá trị dự đoán âm tính của que thử nước tiểu và tính an toàn của việc điều trị đồng thời bằng kháng sinh với corticosteroid liều cao. KẾT QUẢ Tỷ lệ vi khuẩn niệu đáng kể (≥10(5) vi khuẩn/ml) trong nhóm dân số này là 11% (95% CI, 7,1-14,9). Độ đặc hiệu và độ nhạy của este leucocyte dương tính hoặc nitrit là 78% và 65%. Giá trị dự đoán âm tính của que thử nước tiểu là 96%. Không xảy ra bất kỳ tác dụng phụ lâm sàng nào ở 3% (95% CI, 0,9-5,1) bệnh nhân có que thử âm tính giả. Mười tám phần trăm bệnh nhân được điều trị không cần thiết bằng kháng sinh trong 48 giờ. KẾT LUẬN Xét nghiệm que thử nước tiểu cho phép quản lý nhanh chóng và an toàn cho những bệnh nhân bị tái phát MS cấp tính. Thuật toán này bảo thủ và cần có thêm nghiên cứu trong tương lai để giảm tỷ lệ dương tính giả."} +{"idx": 1980128, "en": "To improve the efficiency and to investigate the molecular determinants that direct substrate specificity of chlorocatechol 2,3-dioxygenase CbzE(GJ31) , several mutant enzymes were constructed. Loci for substitutions of amino acids were selected by sequence comparisons as well as by homology modelling of known chlorocatechol 2,3-dioxygenases (CbzE(BASF) , CbzE(SK1) and CbzE(16-6A)). Activity measurements with various catechols showed that most of the modifications influenced activity only to a minor degree. The amino acid at position 154 seems to be located at a non-important position in the enzyme with minor extension into the substrate tunnel. Similarly, the change of related amino acids such as D95E and Y223F did not influence the catalysis since both residues are far away from the catalytic centre and the substrate tunnel. Even the modification of isoleucine to threonine in position 310, located at the outer substrate tunnel, showed a significant alteration of activities. Position 196 seems to be of higher relevance since the modification of valine to alanine, i.e. the reduction of the side-chain, produced much alteration. The amino acid is located at the interface of inner to outer substrate tunnel. CbzE(V196A) showed high relative k(cat) for 3-chlorocatechol. A pronounced increase in activity for 3-chlorocatechol resulted by the change from alanine to valine and from aspartic acid to glycine laying in the outer substrate tunnel at position 211 and 212 respectively.", "gemini": "Để cải thiện hiệu quả và nghiên cứu các yếu tố quyết định phân tử hướng đến tính đặc hiệu của chất nền của chlorocatechol 2,3-dioxygenase CbzE(GJ31), một số enzyme đột biến đã được xây dựng. Các vị trí thay thế axit amin được lựa chọn bằng cách so sánh trình tự cũng như bằng cách lập mô hình đồng đẳng của các chlorocatechol 2,3-dioxygenase đã biết (CbzE(BASF), CbzE(SK1) và CbzE(16-6A)). Các phép đo hoạt động với nhiều loại catechol khác nhau cho thấy hầu hết các sửa đổi chỉ ảnh hưởng đến hoạt động ở mức độ nhỏ. Axit amin ở vị trí 154 có vẻ như nằm ở vị trí không quan trọng trong enzyme với phần mở rộng nhỏ vào đường hầm chất nền. Tương tự như vậy, sự thay đổi của các axit amin liên quan như D95E và Y223F không ảnh hưởng đến quá trình xúc tác vì cả hai chất đều ở xa tâm xúc tác và đường hầm chất nền. Ngay cả việc sửa đổi isoleucine thành threonine ở vị trí 310, nằm ở đường hầm chất nền bên ngoài, cũng cho thấy sự thay đổi đáng kể về hoạt động. Vị trí 196 có vẻ có liên quan cao hơn vì việc sửa đổi valine thành alanine, tức là giảm chuỗi bên, đã tạo ra nhiều thay đổi. Axit amin nằm ở giao diện của đường hầm chất nền bên trong đến bên ngoài. CbzE(V196A) cho thấy k(cat) tương đối cao đối với 3-chlorocatechol. Hoạt động tăng rõ rệt đối với 3-chlorocatechol do sự thay đổi từ alanine thành valine và từ axit aspartic thành glycine nằm ở đường hầm chất nền bên ngoài ở vị trí 211 và 212."} +{"idx": 1980129, "en": "Warm ischemia-reperfusion injury related to donation after cardiac death donors is a crucial and inevitable issue. As surfactant function is known to deteriorate during warm ischemia, we hypothesized that surfactant inhalation during warm ischemia would mitigate warm ischemia-reperfusion injury. We used an isolated rat lung perfusion model. The rats were divided into three groups: sham, control, and surfactant. In the control and surfactant groups, cardiac arrest was induced by ventricular fibrillation. Ventilation was restarted 110 min later; subsequently, the lungs were flushed, and heart and lung block was recovered. In the surfactant group, a natural bovine surfactant Surfacten(®) was inhaled for 3 min at the end of warm ischemia. Then, the lungs were reperfused for 80 min. Surfactant inhalation significantly improved graft functions, effectively increased lung tissue ATP levels, and significantly decreased mRNA levels of IL-6 and IL-6/IL-10 ratio at the end of reperfusion. Histologically, lungs in the surfactant group showed fewer signs of interstitial edema and hemorrhage, and significantly less neutrophilic infiltration than those in the control group. Our results indicated that surfactant inhalation in the last phase of warm ischemia maintained lung tissue energy levels and prevented cytokine production, resulting in the alleviation of warm ischemia-reperfusion injury.", "gemini": "Tổn thương thiếu máu cục bộ-tái tưới máu ấm liên quan đến hiến tặng sau khi chết tim là một vấn đề quan trọng và không thể tránh khỏi. Vì chức năng chất hoạt động bề mặt được biết là xấu đi trong quá trình thiếu máu cục bộ ấm, chúng tôi đưa ra giả thuyết rằng việc hít chất hoạt động bề mặt trong quá trình thiếu máu cục bộ ấm sẽ làm giảm tổn thương thiếu máu cục bộ-tái tưới máu ấm. Chúng tôi đã sử dụng mô hình tưới máu phổi cô lập của chuột. Những con chuột được chia thành ba nhóm: giả, đối chứng và chất hoạt động bề mặt. Ở nhóm đối chứng và nhóm chất hoạt động bề mặt, ngừng tim được gây ra bởi rung thất. Thông khí được khởi động lại 110 phút sau đó; sau đó, phổi được rửa sạch và khối tim và phổi được phục hồi. Ở nhóm chất hoạt động bề mặt, chất hoạt động bề mặt bò tự nhiên Surfacten(®) được hít vào trong 3 phút vào cuối giai đoạn thiếu máu cục bộ ấm. Sau đó, phổi được tái tưới máu trong 80 phút. Việc hít chất hoạt động bề mặt cải thiện đáng kể chức năng ghép, làm tăng hiệu quả nồng độ ATP của mô phổi và làm giảm đáng kể nồng độ mRNA của IL-6 và tỷ lệ IL-6/IL-10 vào cuối giai đoạn tái tưới máu. Về mặt mô học, phổi ở nhóm chất hoạt động bề mặt cho thấy ít dấu hiệu phù kẽ và xuất huyết hơn và thâm nhiễm bạch cầu trung tính ít hơn đáng kể so với nhóm đối chứng. Kết quả của chúng tôi chỉ ra rằng việc hít chất hoạt động bề mặt trong giai đoạn cuối của thiếu máu cục bộ ấm duy trì mức năng lượng của mô phổi và ngăn ngừa sản xuất cytokine, dẫn đến giảm tổn thương thiếu máu cục bộ-tái tưới máu ấm."} +{"idx": 1980130, "en": "OBJECTIVE To examine the association between bodyweight status and provision of population-based prevention services. DATA SOURCES The National Association of City and County Health Officials 2005 Profile survey data, linked with two cross-sections of the Behavioral Risk Factor Surveillance System (BRFSS) survey in 2004 and 2005. STUDY DESIGN Multilevel logistic regressions were used to examine the association between provision of obesity-prevention services and the change in risk of being obese or morbidly obese among BRFSS respondents. The estimation sample was stratified by sex. Low-income samples were also examined. Falsification tests were used to determine whether there is counterevidence. PRINCIPAL FINDINGS Provision of population-based obesity-prevention services within the jurisdiction of local health departments and specifically those provided by the local health departments are associated with reduced risks of obesity and morbid obesity from 2004 to 2005. The magnitude of the association appears to be stronger among low-income populations and among women. Results of the falsification tests provide additional support of the main findings. CONCLUSIONS Population-based obesity-prevention services may be useful in containing the obesity epidemic.", "gemini": "MỤC TIÊU Kiểm tra mối liên quan giữa tình trạng cân nặng và việc cung cấp các dịch vụ phòng ngừa dựa trên dân số. NGUỒN DỮ LIỆU Dữ liệu khảo sát Hồ sơ năm 2005 của Hiệp hội các quan chức y tế thành phố và quận quốc gia, được liên kết với hai mặt cắt ngang của cuộc khảo sát Hệ thống giám sát yếu tố nguy cơ hành vi (BRFSS) vào năm 2004 và 2005. THIẾT KẾ NGHIÊN CỨU Các hồi quy logistic đa cấp được sử dụng để kiểm tra mối liên quan giữa việc cung cấp các dịch vụ phòng ngừa béo phì và sự thay đổi nguy cơ béo phì hoặc béo phì bệnh hoạn ở những người trả lời BRFSS. Mẫu ước tính được phân tầng theo giới tính. Các mẫu có thu nhập thấp cũng được kiểm tra. Các bài kiểm tra làm sai lệch được sử dụng để xác định xem có bằng chứng phản biện hay không. KẾT LUẬN CHÍNH Cung cấp các dịch vụ phòng ngừa béo phì dựa trên dân số trong phạm vi quyền hạn của các sở y tế địa phương và đặc biệt là các dịch vụ do các sở y tế địa phương cung cấp có liên quan đến việc giảm nguy cơ béo phì và béo phì bệnh hoạn từ năm 2004 đến năm 2005. Quy mô của mối liên quan có vẻ mạnh hơn ở những người có thu nhập thấp và ở phụ nữ. Kết quả của các bài kiểm tra làm sai lệch cung cấp thêm bằng chứng hỗ trợ cho những phát hiện chính. KẾT LUẬN Các dịch vụ phòng ngừa béo phì dựa trên dân số có thể hữu ích trong việc ngăn chặn đại dịch béo phì."} +{"idx": 1980131, "en": "Dabbling ducks, particularly Mallards (Anas platyrhynchos) have been frequently and consistently reported to play a pivotal role as a reservoir of low pathogenic avian influenza viruses (AIV). From October 2006 to November 2008, hand-raised Mallard ducks kept at a pond in an avifaunistically rich area of Southern Germany served as sentinel birds in the AIV surveillance programme in Germany. The pond was regularly visited by several species of dabbling ducks. A flock of sentinel birds, consisting of the same 16 individual birds during the whole study period, was regularly tested virologically and serologically for AIV infections. Swab samples were screened by RT-qPCR and, if positive, virus was isolated in embryonated chicken eggs. Serum samples were tested by the use of competitive ELISA and hemagglutinin inhibition (HI) assay. Sequences of full-length hemagglutinin (HA) and neuraminidase (NA) genes were phylogenetically analysed. Four episodes of infections with Eurasian-type AIV occurred in August (H6N8), October/November (H3N2, H2N3) 2007, in January (H3N2) and September (H3N8) 2008. The HA and NA genes of the H3N2 viruses of October 2007 and January 2008 were almost identical rendering the possibility of a re-introduction of that virus from the environment of the sentinel flock highly likely. The HA of the H3N8 virus of September 2008 belonged to a different cluster. As a correlate of the humoral immune response, titres of nucleocapsid protein-specific antibodies fluctuated in correlation with the course of AIV infection episodes. However, no specific systemic response of hemagglutination inhibiting antibodies could be demonstrated even if homologous viral antigens were used. Besides being useful as early indicators for the circulation of influenza viruses in a specific region, the sentinel ducks also contributed to gaining insights into the ecobiology of AIV infection in aquatic wild birds.", "gemini": "Vịt dabbling, đặc biệt là vịt trời (Anas platyrhynchos) đã thường xuyên và liên tục được báo cáo đóng vai trò quan trọng như một ổ chứa virus cúm gia cầm độc lực thấp (AIV). Từ tháng 10 năm 2006 đến tháng 11 năm 2008, những con vịt trời nuôi bằng tay được nuôi tại một ao ở một khu vực giàu chim ở miền Nam nước Đức đóng vai trò là chim gác trong chương trình giám sát AIV tại Đức. Ao thường xuyên có sự ghé thăm của một số loài vịt dabbling. Một đàn chim gác, bao gồm cùng 16 cá thể chim trong suốt thời gian nghiên cứu, được kiểm tra thường xuyên về mặt vi rút học và huyết thanh học đối với các bệnh nhiễm AIV. Các mẫu tăm bông được sàng lọc bằng RT-qPCR và nếu dương tính, vi-rút sẽ được phân lập trong trứng gà có phôi. Các mẫu huyết thanh được kiểm tra bằng cách sử dụng ELISA cạnh tranh và xét nghiệm ức chế ngưng kết hồng cầu (HI). Trình tự của các gen hemagglutinin (HA) và neuraminidase (NA) toàn phần được phân tích về mặt phát sinh chủng loài. Bốn đợt nhiễm trùng AIV týp Á-Âu xảy ra vào tháng 8 (H6N8), tháng 10/tháng 11 (H3N2, H2N3) năm 2007, vào tháng 1 (H3N2) và tháng 9 (H3N8) năm 2008. Các gen HA và NA của vi-rút H3N2 tháng 10 năm 2007 và tháng 1 năm 2008 gần như giống hệt nhau, khiến khả năng tái xâm nhập vi-rút đó từ môi trường của đàn chim gác là rất cao. HA của vi-rút H3N8 tháng 9 năm 2008 thuộc về một nhóm khác. Là một tương quan của phản ứng miễn dịch dịch thể, nồng độ kháng thể đặc hiệu với protein nucleocapsid dao động tương quan với diễn biến của các đợt nhiễm trùng AIV. Tuy nhiên, không thể chứng minh được phản ứng toàn thân đặc hiệu nào của các kháng thể ức chế ngưng kết hồng cầu ngay cả khi sử dụng các kháng nguyên vi-rút đồng loại. Bên cạnh việc hữu ích như những chỉ báo sớm về sự lưu hành của vi-rút cúm ở một khu vực cụ thể, những con vịt gác cũng góp phần giúp hiểu sâu hơn về sinh thái của bệnh nhiễm AIV ở các loài chim hoang dã thủy sinh."} +{"idx": 1980132, "en": "Ecosystems provide services necessary for the livelihoods and well-being of people. Quantifying and mapping supplies and demands of ecosystem services is essential for continuous monitoring of such services to support decision-making. Area-wide and spatially explicit mapping of ecosystem services based on extensive ground surveys is restricted to local scales and limited due to high costs. In contrast, remote sensing provides reliable area-wide data for quantifying and mapping ecosystem services at comparatively low costs, and with the option of fast, frequent, and continuous observations for monitoring. In this paper, we review relevant remote sensing systems, sensor types, and methods applicable in quantifying selected provisioning and regulatory services. Furthermore, opportunities, challenges, and future prospects in using remote sensing for supporting ecosystem services' quantification and mapping are discussed.", "gemini": "Các hệ sinh thái cung cấp các dịch vụ cần thiết cho sinh kế và sức khỏe của người dân. Việc định lượng và lập bản đồ cung và cầu của các dịch vụ hệ sinh thái là rất cần thiết để theo dõi liên tục các dịch vụ đó nhằm hỗ trợ cho việc ra quyết định. Việc lập bản đồ các dịch vụ hệ sinh thái trên toàn khu vực và theo không gian dựa trên các cuộc khảo sát thực địa mở rộng bị hạn chế ở quy mô địa phương và bị hạn chế do chi phí cao. Ngược lại, viễn thám cung cấp dữ liệu đáng tin cậy trên toàn khu vực để định lượng và lập bản đồ các dịch vụ hệ sinh thái với chi phí tương đối thấp và có tùy chọn quan sát nhanh, thường xuyên và liên tục để theo dõi. Trong bài báo này, chúng tôi xem xét các hệ thống viễn thám có liên quan, các loại cảm biến và các phương pháp có thể áp dụng để định lượng các dịch vụ cung cấp và điều tiết đã chọn. Hơn nữa, các cơ hội, thách thức và triển vọng trong tương lai trong việc sử dụng viễn thám để hỗ trợ định lượng và lập bản đồ các dịch vụ hệ sinh thái được thảo luận."} +{"idx": 1980133, "en": "Atomistic molecular dynamics simulations of proteins in aqueous solution are still limited to the multinanosecond time scale and multinanometer range by computational cost. Combining atomic solutes with a supra-molecular solvent model in hybrid fine-grained/coarse-grained (FG/CG) simulations allows atomic detail in the region of interest while being computationally more efficient. A recent comparison of the properties of four proteins in CG water versus FG water showed the preservation of the secondary and tertiary structure with a computational speed-up of at least an order of magnitude. However, an increased occurrence of hydrogen bonds between side chains was observed due to a lack of hydrogen-bonding partners in the supra-molecular solvent. Here, the introduction of a FG water layer around the protein to recover the hydrogen-bonding pattern of the atomistic simulations is studied. Three layer thicknesses of 0.2, 0.4, and 0.8 nm are considered. A layer thickness of 0.8 nm is found sufficient to recover the behavior of the proteins in the atomistic simulations, whereas the hybrid simulation is still three times more efficient than the atomistic one and the cutoff radius for nonbonded interactions could be increased from 1.4 to 2.0 nm.", "gemini": "Mô phỏng động lực học phân tử nguyên tử của protein trong dung dịch nước vẫn còn giới hạn ở thang thời gian nhiều nano giây và phạm vi nhiều nanomet do chi phí tính toán. Kết hợp các chất tan nguyên tử với mô hình dung môi siêu phân tử trong các mô phỏng hạt mịn/hạt thô (FG/CG) lai cho phép chi tiết nguyên tử trong khu vực quan tâm trong khi hiệu quả hơn về mặt tính toán. Một so sánh gần đây về các đặc tính của bốn protein trong nước CG so với nước FG cho thấy sự bảo toàn cấu trúc bậc hai và bậc ba với tốc độ tính toán nhanh hơn ít nhất một bậc độ lớn. Tuy nhiên, người ta đã quan sát thấy sự gia tăng liên kết hydro giữa các chuỗi bên do thiếu các đối tác liên kết hydro trong dung môi siêu phân tử. Ở đây, việc đưa vào một lớp nước FG xung quanh protein để phục hồi kiểu liên kết hydro của các mô phỏng nguyên tử được nghiên cứu. Ba độ dày lớp là 0,2, 0,4 và 0,8 nm được xem xét. Độ dày lớp 0,8 nm được thấy là đủ để phục hồi hành vi của các protein trong các mô phỏng nguyên tử, trong khi mô phỏng lai vẫn hiệu quả hơn ba lần so với mô phỏng nguyên tử và bán kính cắt cho các tương tác không liên kết có thể tăng từ 1,4 lên 2,0 nm."} +{"idx": 1980134, "en": "BACKGROUND Little is known about health providers' attitudes toward visual inspection with acetic acid (VIA) and cryotherapy in the prevention of cervical cancer, as most research in Latin America and the Caribbean (LAC) has examined attitudes of the general population. This study describes attitudes of Bolivian health professionals toward new technologies for cervical cancer prevention, focusing on VIA and cryotherapy. METHODS Between February 2011 and March 2012, we surveyed 7 nurses and 35 physicians who participated in 5-day workshops on VIA and cryotherapy conducted in Bolivia. Multiple choice and open-ended questions were used to assess participants' acceptability of these procedures and the feasibility of their implementation in the context of perceived barriers for the early detection of cervical cancer in this country. RESULTS Most believed that cultural factors represent the main barrier for the early detection of cervical cancer (70%), although all stated that VIA and cryotherapy would be accepted by women, citing the advantages of VIA over cytology for this belief. Most also believed their colleagues would accept VIA and cryotherapy (71%) and that VIA should replace Pap testing (61%), reiterating the advantages of VIA for these beliefs. Those who believed the contrary expressed a general resistance to change associated with an already existing cytology program and national norms prioritizing Pap testing. CONCLUSIONS Most participants had favorable attitudes toward VIA and cryotherapy; however, a sizable minority cited challenges to their adoption by colleagues and believed VIA should not replace cytology. This report can inform the development of strategies to expand the use of alternative cervical cancer screening methods in LAC and Bolivia.", "gemini": "BỐI CẢNH Người ta biết rất ít về thái độ của các nhà cung cấp dịch vụ y tế đối với việc kiểm tra trực quan bằng axit axetic (VIA) và liệu pháp đông lạnh trong việc phòng ngừa ung thư cổ tử cung, vì hầu hết các nghiên cứu ở Mỹ Latinh và Caribe (LAC) đã xem xét thái độ của dân chúng nói chung. Nghiên cứu này mô tả thái độ của các chuyên gia y tế Bolivia đối với các công nghệ mới để phòng ngừa ung thư cổ tử cung, tập trung vào VIA và liệu pháp đông lạnh. PHƯƠNG PHÁP Trong khoảng thời gian từ tháng 2 năm 2011 đến tháng 3 năm 2012, chúng tôi đã khảo sát 7 y tá và 35 bác sĩ đã tham gia các hội thảo kéo dài 5 ngày về VIA và liệu pháp đông lạnh được tiến hành tại Bolivia. Các câu hỏi trắc nghiệm và câu hỏi mở đã được sử dụng để đánh giá mức độ chấp nhận của những người tham gia đối với các thủ thuật này và khả năng thực hiện của họ trong bối cảnh các rào cản nhận thức đối với việc phát hiện sớm ung thư cổ tử cung ở quốc gia này. KẾT QUẢ Hầu hết đều tin rằng các yếu tố văn hóa là rào cản chính đối với việc phát hiện sớm ung thư cổ tử cung (70%), mặc dù tất cả đều nêu rõ rằng VIA và liệu pháp đông lạnh sẽ được phụ nữ chấp nhận, trích dẫn những ưu điểm của VIA so với tế bào học vì niềm tin này. Hầu hết cũng tin rằng các đồng nghiệp của họ sẽ chấp nhận VIA và liệu pháp đông lạnh (71%) và VIA nên thay thế xét nghiệm Pap (61%), nhắc lại những ưu điểm của VIA đối với những niềm tin này. Những người tin ngược lại bày tỏ sự phản đối chung đối với sự thay đổi liên quan đến chương trình tế bào học đã có và các chuẩn mực quốc gia ưu tiên xét nghiệm Pap. KẾT LUẬN Hầu hết những người tham gia đều có thái độ thuận lợi đối với VIA và liệu pháp đông lạnh; tuy nhiên, một số ít đáng kể đã nêu ra những thách thức đối với việc áp dụng của các đồng nghiệp và tin rằng VIA không nên thay thế tế bào học. Báo cáo này có thể cung cấp thông tin cho việc phát triển các chiến lược nhằm mở rộng việc sử dụng các phương pháp sàng lọc ung thư cổ tử cung thay thế ở LAC và Bolivia."} +{"idx": 1980135, "en": "Individual chromatin molecules contain valuable genetic and epigenetic information. To date, there have not been reliable techniques available for the controlled stretching and manipulation of individual chromatin fragments for high-resolution imaging and analysis of these molecules. We report the controlled stretching of single chromatin fragments extracted from two different cancerous cell types (M091 and HeLa) characterized through fluorescence microscopy and atomic force microscopy (AFM). Our method combines soft lithography with molecular stretching to form ordered arrays of more than 250,000 individual chromatin fragments immobilized into a beads-on-a-string structure on a solid transparent support. Using fluorescence microscopy and AFM, we verified the presence of histone proteins after the stretching and transfer process.", "gemini": "Các phân tử chromatin riêng lẻ chứa thông tin di truyền và biểu sinh có giá trị. Cho đến nay, vẫn chưa có các kỹ thuật đáng tin cậy nào có sẵn để kéo căng và thao tác có kiểm soát các đoạn chromatin riêng lẻ để hình ảnh và phân tích độ phân giải cao các phân tử này. Chúng tôi báo cáo về việc kéo căng có kiểm soát các đoạn chromatin đơn lẻ được chiết xuất từ hai loại tế bào ung thư khác nhau (M091 và HeLa) được đặc trưng thông qua kính hiển vi huỳnh quang và kính hiển vi lực nguyên tử (AFM). Phương pháp của chúng tôi kết hợp thạch bản mềm với kéo căng phân tử để tạo thành các mảng có trật tự gồm hơn 250.000 đoạn chromatin riêng lẻ được cố định thành cấu trúc hạt trên dây trên giá đỡ trong suốt rắn. Sử dụng kính hiển vi huỳnh quang và AFM, chúng tôi đã xác minh sự hiện diện của protein histone sau quá trình kéo căng và chuyển."} +{"idx": 1980136, "en": "BACKGROUND Wound closure after excision is commonly done with sutures or staples. A new sutureless innovative wound closure system is available for sutureless skin closure. OBJECTIVE To evaluate wound healing, patient comfort, and cosmetic results of a foil flip-over system for excision of small skin lesion. MATERIALS AND METHODS Patients presenting to the department of Dermatology of Erasmus University Medical Center, Rotterdam, The Netherlands for skin surgery during a 1.5-year period were prospectively studied. Key outcome measures were wound healing, patient comfort, and cosmetic results. Three independent physicians scored photographs of the scars. Evaluation tools used were comfort and body image questionnaires and visual analogue scales. RESULTS Ninety-six patients with 103 lesions were included in our study. The surgeon scored wound healing as excellent or good in 96%. No wound infections occurred. Ninety-two percent of patients scored removal of the system as comfortable. Median patient grade of scar after 1 month was 8 out of 10 points (interquartile range [IQR] 7-9). Median independent physician grade of photographs of the scars was 7.7 (IQR 7.1-8.0). CONCLUSION Sutureless foil flip-over is promising, with excellent patient comfort characteristics and good to excellent cosmetic results.", "gemini": "BỐI CẢNH Việc đóng vết thương sau khi cắt bỏ thường được thực hiện bằng chỉ khâu hoặc kim bấm. Một hệ thống đóng vết thương không cần khâu mới hiện có để đóng da không cần khâu. MỤC TIÊU Đánh giá quá trình lành vết thương, sự thoải mái của bệnh nhân và kết quả thẩm mỹ của hệ thống lật giấy bạc để cắt bỏ tổn thương da nhỏ. VẬT LIỆU VÀ PHƯƠNG PHÁP Bệnh nhân đến khoa Da liễu của Trung tâm Y tế Đại học Erasmus, Rotterdam, Hà Lan để phẫu thuật da trong thời gian 1,5 năm đã được nghiên cứu có triển vọng. Các biện pháp kết quả chính là lành vết thương, sự thoải mái của bệnh nhân và kết quả thẩm mỹ. Ba bác sĩ độc lập đã chấm điểm ảnh chụp vết sẹo. Các công cụ đánh giá được sử dụng là bảng câu hỏi về sự thoải mái và hình ảnh cơ thể và thang đo tương tự thị giác. KẾT QUẢ Chín mươi sáu bệnh nhân với 103 tổn thương đã được đưa vào nghiên cứu của chúng tôi. Bác sĩ phẫu thuật đánh giá vết thương lành là xuất sắc hoặc tốt ở mức 96%. Không xảy ra nhiễm trùng vết thương. Chín mươi hai phần trăm bệnh nhân đánh giá việc loại bỏ hệ thống là thoải mái. Điểm trung bình của bệnh nhân về sẹo sau 1 tháng là 8 trên 10 điểm (khoảng tứ phân vị [IQR] 7-9). Điểm trung bình của bác sĩ độc lập về ảnh chụp vết sẹo là 7,7 (IQR 7,1-8,0). KẾT LUẬN Lật giấy bạc không cần khâu rất hứa hẹn, với đặc điểm thoải mái cho bệnh nhân tuyệt vời và kết quả thẩm mỹ từ tốt đến xuất sắc."} +{"idx": 1980137, "en": "Porcine reproductive and respiratory syndrome virus (PRRSV) can persist in different organs of infected pigs, which suggests a failure in the immune response. Antigen-presenting cells (APCs) play a pivotal role in the induction of effective T- and B-cell responses. In this study, we investigated the changes in the different APC subpopulations and T- and B-cell counts in the tonsil, retropharyngeal and mediastinal lymph nodes of pigs experimentally infected with a European PRRSV field isolate. Our results demonstrated that the expression of S100, SWC3, HLA-DR molecule and CD3 was diminished in the studied organs throughout the study, observing a significant negative correlation between viral antigen and HLA-DR expression in both retropharyngeal and mediastinal lymph nodes. In contrast, λ-light chains showed an increase during the study. Taking all into account, after PRRSV infection, no enhancement in the number of APCs and T cells was observed, suggesting an impairment of the immune function which may allow the persistence of PRRSV into the organism.", "gemini": "Virus gây hội chứng sinh sản và hô hấp ở lợn (PRRSV) có thể tồn tại ở các cơ quan khác nhau của lợn bị nhiễm, điều này cho thấy sự thất bại trong phản ứng miễn dịch. Các tế bào trình diện kháng nguyên (APC) đóng vai trò quan trọng trong việc tạo ra các phản ứng tế bào T và tế bào B hiệu quả. Trong nghiên cứu này, chúng tôi đã điều tra những thay đổi trong các phân nhóm APC khác nhau và số lượng tế bào T và tế bào B ở amidan, hạch bạch huyết retropharyngeal và trung thất của lợn bị nhiễm thực nghiệm bằng một phân lập thực địa PRRSV châu Âu. Kết quả của chúng tôi chứng minh rằng sự biểu hiện của phân tử S100, SWC3, HLA-DR và CD3 đã giảm ở các cơ quan được nghiên cứu trong suốt quá trình nghiên cứu, quan sát thấy mối tương quan âm đáng kể giữa kháng nguyên vi rút và biểu hiện HLA-DR ở cả hạch bạch huyết retropharyngeal và trung thất. Ngược lại, chuỗi nhẹ λ cho thấy sự gia tăng trong quá trình nghiên cứu. Xét đến tất cả các yếu tố, sau khi nhiễm PRRSV, không quan sát thấy sự gia tăng số lượng APC và tế bào T, điều này cho thấy sự suy giảm chức năng miễn dịch có thể cho phép PRRSV tồn tại trong cơ thể."} +{"idx": 1980138, "en": "Plant species exhibit a range of tolerances to low temperatures, and these constitute a major determinant of their geographical distribution and use as crops. When tolerance is insufficient, either chilling or freezing injuries result. A variety of mechanisms are employed to evade the ravages of extreme or sub-optimal temperatures. Many of these involve cold-responsive gene expression and require that the drop in temperature is first sensed by the plant. Despite intensive research over the last 100 yr or longer, we still cannot easily answer the question of how plants sense low temperature. Over recent years, genomic and post-genomic approaches have produced a wealth of information relating to the sequence of events leading from cold perception to appropriate and useful responses. However, there are also crucial and significant gaps in the pathways constructed from these data. We describe the literature pertaining to the current understanding of cold perception, signalling and regulation of low-temperature-responsive gene expression in higher plants, raising some of the key questions that still intrigue plant biologists today and that could be targets for future work. Our review focuses on the control of gene expression in the pathways leading from cold perception to chilling and freezing tolerance.", "gemini": "Các loài thực vật biểu hiện một loạt các mức chịu đựng đối với nhiệt độ thấp và đây là yếu tố quyết định chính đến sự phân bố địa lý và việc sử dụng chúng làm cây trồng. Khi khả năng chịu đựng không đủ, có thể dẫn đến tình trạng thương tổn do lạnh hoặc đóng băng. Có nhiều cơ chế được sử dụng để tránh khỏi sự tàn phá của nhiệt độ khắc nghiệt hoặc không tối ưu. Nhiều cơ chế trong số này liên quan đến biểu hiện gen đáp ứng lạnh và yêu cầu sự sụt giảm nhiệt độ trước tiên phải được cây cảm nhận. Mặc dù đã có những nghiên cứu chuyên sâu trong hơn 100 năm trở lại đây, chúng ta vẫn không thể dễ dàng trả lời câu hỏi làm thế nào mà thực vật cảm nhận được nhiệt độ thấp. Trong những năm gần đây, các phương pháp tiếp cận về hệ gen và hậu hệ gen đã tạo ra rất nhiều thông tin liên quan đến trình tự các sự kiện dẫn từ nhận thức lạnh đến các phản ứng thích hợp và hữu ích. Tuy nhiên, cũng có những lỗ hổng quan trọng và đáng kể trong các con đường được xây dựng từ các dữ liệu này. Chúng tôi mô tả các tài liệu liên quan đến sự hiểu biết hiện tại về nhận thức lạnh, tín hiệu và điều hòa biểu hiện gen đáp ứng nhiệt độ thấp ở thực vật bậc cao, nêu ra một số câu hỏi chính vẫn khiến các nhà sinh học thực vật ngày nay thích thú và có thể là mục tiêu cho các công trình nghiên cứu trong tương lai. Đánh giá của chúng tôi tập trung vào việc kiểm soát biểu hiện gen trong các con đường dẫn từ nhận thức lạnh đến khả năng chịu lạnh và đóng băng."} +{"idx": 1980139, "en": "The main surgical changes in kidney procurement, preparation, and transplantation procedures occurred 20 years ago and were undertaken despite the inability to design randomized studies. The objective was to assess the evolution of vascular complications after kidney transplantation in a setting of surgical preventive measures in a historical series. A monocentric series of 3129 consecutive kidney transplantations performed over 3 decades was reviewed. The occurrence of arterial or venous thromboses, stenoses, and aneurysms was analyzed in relation with kidney procurement, preparation, and transplantation techniques. Vascular complications occurred in 13.5% of the recipients with a mean 3-year decrease in kidney graft function. The transplantation of a right kidney without renal vein extension, multiple renal arteries, ex vivo vascular repairs, and end-to-end arterial anastomoses were the unfavorable surgical vascular factors. It was possible to manage Transplant Renal Artery Stenosis (TRAS) nonsurgically in 80% of the cases. The prevention of vascular complications begins from the time of organ procurement by skilled surgeons. The aims of organ preparation are to evaluate the vascular risk, select the organs, and to simplify the anatomical constraints of vascular implantations. The three surgical steps of kidney transplantation are determinant in postoperative vascular complications and the duration of graft function.", "gemini": "Những thay đổi phẫu thuật chính trong các thủ thuật lấy, chuẩn bị và ghép thận đã diễn ra cách đây 20 năm và được thực hiện mặc dù không thể thiết kế các nghiên cứu ngẫu nhiên. Mục tiêu là đánh giá sự tiến triển của các biến chứng mạch máu sau khi ghép thận trong bối cảnh các biện pháp phòng ngừa phẫu thuật trong một loạt các trường hợp có tiền sử. Một loạt các ca ghép thận liên tiếp gồm 3129 ca được thực hiện trong hơn 3 thập kỷ đã được xem xét. Xảy ra huyết khối động mạch hoặc tĩnh mạch, hẹp và phình động mạch được phân tích liên quan đến các kỹ thuật lấy, chuẩn bị và ghép thận. Biến chứng mạch máu xảy ra ở 13,5% người nhận với chức năng ghép thận giảm trung bình sau 3 năm. Việc ghép thận phải mà không kéo dài tĩnh mạch thận, nhiều động mạch thận, sửa chữa mạch máu ngoài cơ thể và nối động mạch tận cùng là những yếu tố mạch máu phẫu thuật không thuận lợi. Có thể quản lý Hẹp động mạch thận ghép (TRAS) không phẫu thuật ở 80% các trường hợp. Phòng ngừa biến chứng mạch máu bắt đầu từ thời điểm lấy nội tạng bởi các bác sĩ phẫu thuật có tay nghề. Mục đích của việc chuẩn bị nội tạng là đánh giá rủi ro mạch máu, lựa chọn các cơ quan và đơn giản hóa các ràng buộc giải phẫu của việc cấy ghép mạch máu. Ba bước phẫu thuật ghép thận là yếu tố quyết định đến các biến chứng mạch máu sau phẫu thuật và thời gian hoạt động của ghép."} +{"idx": 1980140, "en": "While agricultural residue is considered as a near-term feedstock option for cellulosic biofuels, its sustainability must be evaluated by taking water into account. This study aims to analyze the county-level water footprint for four biofuel pathways in the United States, including bioethanol generated from corn grain, stover, wheat straw, and biodiesel from soybean. The county-level blue water footprint of ethanol from corn grain, stover, and wheat straw shows extremely wide variances with a national average of 31, 132, and 139 L of water per liter biofuel (L(w)/L(bf)), and standard deviation of 133, 323, and 297 L(w)/L(bf), respectively. Soybean biodiesel production results in a blue water footprint of 313 L(w)/L(bf) on the national average with standard deviation of 894 L(w)/L(bf). All biofuels show a greater green water footprint than the blue one. This work elucidates how diverse spatial resolutions affect biofuel water footprints, which can provide detailed insights into biofuels' implications on local water sustainability.", "gemini": "Trong khi dư lượng nông nghiệp được coi là một lựa chọn nguồn nguyên liệu gần cho nhiên liệu sinh học xenluloza, tính bền vững của nó phải được đánh giá bằng cách tính đến nước. Nghiên cứu này nhằm phân tích dấu chân nước ở cấp quận cho bốn con đường nhiên liệu sinh học ở Hoa Kỳ, bao gồm bioethanol được tạo ra từ hạt ngô, rơm rạ, rơm lúa mì và biodiesel từ đậu nành. Dấu chân nước xanh ở cấp quận đối với ethanol từ hạt ngô, rơm rạ và rơm lúa mì cho thấy sự chênh lệch cực kỳ lớn với mức trung bình toàn quốc là 31, 132 và 139 L nước trên một lít nhiên liệu sinh học (L(w)/L(bf)) và độ lệch chuẩn là 133, 323 và 297 L(w)/L(bf), tương ứng. Sản xuất biodiesel từ đậu nành dẫn đến dấu chân nước xanh là 313 L(w)/L(bf) trên mức trung bình toàn quốc với độ lệch chuẩn là 894 L(w)/L(bf). Tất cả các nhiên liệu sinh học đều cho thấy dấu chân nước xanh lớn hơn dấu chân nước xanh. Công trình này làm sáng tỏ cách các độ phân giải không gian khác nhau ảnh hưởng đến dấu chân nước của nhiên liệu sinh học, có thể cung cấp những hiểu biết chi tiết về tác động của nhiên liệu sinh học đối với tính bền vững của nguồn nước tại địa phương."} +{"idx": 1980141, "en": "In somatic cell nuclear transfer (SCNT) procedures, exquisite enucleation of the recipient oocyte is critical to cloning efficiency. The purpose of this study was to compare the effects of two enucleation systems, Hoechst staining and UV irradiation (hereafter, irradiation group) and Oosight imaging (hereafter, Oosight group), on the in vitro production of bovine SCNT embryos. In the Oosight group, the apoptotic index (2.8 ± 0.5 vs. 7.3 ± 1.2) was lower, and the fusion rate (75.6% vs. 62.9%), cleavage rate (78.0% vs. 63.7%), blastocyst rate (40.2% vs. 29.2%), and total cell number (128.3±4.8 vs. 112.2 ± 7.6) were higher than those in the irradiation group (all p<0.05). The overall efficiency after SCNT was twice as high in the Oosight group as that in the irradiation group (p<0.05). The relative mRNA expression levels of Oct4, Nanog, Interferon-tau, and Dnmt3A were higher and those of Caspase-3 and Hsp70 were lower in the Oosight group compared with the irradiation group (p<0.05). This is the first report to show the positive effect of the Oosight imaging system on molecular gene expression in the SCNT embryo. The Oosight imaging system may become the preferred choice for enucleation because it is less detrimental to the developmental potential of bovine SCNT embryos.", "gemini": "Trong các quy trình chuyển nhân tế bào soma (SCNT), việc loại bỏ nhân chính xác của tế bào trứng nhận là rất quan trọng đối với hiệu quả nhân bản. Mục đích của nghiên cứu này là so sánh các tác động của hai hệ thống loại bỏ nhân, nhuộm Hoechst và chiếu tia UV (sau đây gọi là nhóm chiếu xạ) và chụp ảnh Oosight (sau đây gọi là nhóm Oosight), đối với việc sản xuất phôi SCNT bò trong ống nghiệm. Trong nhóm Oosight, chỉ số tế bào chết theo chương trình (2,8 ± 0,5 so với 7,3 ± 1,2) thấp hơn và tỷ lệ hợp nhất (75,6% so với 62,9%), tỷ lệ phân cắt (78,0% so với 63,7%), tỷ lệ phôi nang (40,2% so với 29,2%) và tổng số tế bào (128,3 ± 4,8 so với 112,2 ± 7,6) cao hơn so với nhóm chiếu xạ (tất cả p < 0,05). Hiệu quả tổng thể sau SCNT cao gấp đôi ở nhóm Oosight so với nhóm chiếu xạ (p < 0,05). Mức biểu hiện mRNA tương đối của Oct4, Nanog, Interferon-tau và Dnmt3A cao hơn và Caspase-3 và Hsp70 thấp hơn ở nhóm Oosight so với nhóm chiếu xạ (p < 0,05). Đây là báo cáo đầu tiên cho thấy tác động tích cực của hệ thống chụp ảnh Oosight đối với biểu hiện gen phân tử trong phôi SCNT. Hệ thống chụp ảnh Oosight có thể trở thành lựa chọn ưu tiên để loại bỏ nhân vì nó ít gây hại hơn đến tiềm năng phát triển của phôi SCNT bò."} +{"idx": 1980142, "en": "BACKGROUND AND PURPOSE The autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS) is an early-onset neurodegenerative disorder caused by mutations in the SACS gene. The disease, first described in Canadian families from Québec, is characterized by cerebellar ataxia, pyramidal tract involvement and peripheral neuropathy. METHODS Analysis of SACS gene allowed the identification of 14 patients with ARSACS from 13 unrelated Italian families. Clinical phenotype, gene mutations and magnetic resonance imaging (MRI) findings were analysed. RESULTS We found 16 novel SACS gene mutations, including a large in-frame deletion. The age at onset was in infancy, but one patient presented the first symptoms at age 32. Progression of the disease was variable, and increased muscle tone was mostly recognized in later stages. Structural MRI showed atrophy of the superior cerebellar vermis, a bulky pons exhibiting T2-hypointense stripes, identified as the corticospinal tract (CST), thinning of the corpus callosum and a rim of T2-hyperintensity around the thalami in 100% of cases. The presence of iron or other paramagnetic substances was excluded. Diffusion tensor imaging (DTI) revealed grossly over-represented transverse pontine fibres (TPF), which prevented reconstruction of the CST at this level (100% of cases). In all patients, significant microstructural alterations were found in the supratentorial white matter of forceps, cingulum and superior longitudinal fasciculus. CONCLUSIONS Our findings further enlarge the genetic spectrum of SACS mutations and widen the study of clinical phenotype. MRI characteristics indicate that pontine changes and supratentorial abnormalities are diagnostic. The over-representation of TPF on DTI suggests a developmental component in the pathogenesis of the disease.", "gemini": "BỐI CẢNH VÀ MỤC ĐÍCH Bệnh thoái hóa thần kinh thể cứng đầu chi nhiễm sắc thể thường trội của Charlevoix-Saguenay (ARSACS) là một rối loạn thoái hóa thần kinh khởi phát sớm do đột biến ở gen SACS. Bệnh này, lần đầu tiên được mô tả ở các gia đình người Canada từ Québec, được đặc trưng bởi chứng mất điều hòa tiểu não, tổn thương đường tháp và bệnh thần kinh ngoại biên. PHƯƠNG PHÁP Phân tích gen SACS cho phép xác định 14 bệnh nhân mắc ARSACS từ 13 gia đình không có quan hệ họ hàng ở Ý. Kiểu hình lâm sàng, đột biến gen và kết quả chụp cộng hưởng từ (MRI) đã được phân tích. KẾT QUẢ Chúng tôi đã tìm thấy 16 đột biến gen SACS mới, bao gồm một đoạn xóa lớn trong khung. Tuổi khởi phát là thời thơ ấu, nhưng một bệnh nhân biểu hiện các triệu chứng đầu tiên ở tuổi 32. Quá trình tiến triển của bệnh rất khác nhau và tăng trương lực cơ chủ yếu được nhận thấy ở giai đoạn sau. MRI cấu trúc cho thấy teo vermis tiểu não trên, cầu não cồng kềnh có các sọc giảm tín hiệu T2, được xác định là đường corticospinal (CST), mỏng thể chai và một vành tăng tín hiệu T2 xung quanh đồi thị ở 100% trường hợp. Sự hiện diện của sắt hoặc các chất thuận từ khác đã bị loại trừ. Chụp ảnh tensor khuếch tán (DTI) cho thấy các sợi ngang cầu (TPF) được biểu diễn quá mức, ngăn cản việc tái tạo CST ở mức này (100% trường hợp). Ở tất cả bệnh nhân, các biến đổi vi cấu trúc đáng kể được tìm thấy ở chất trắng trên lều của kìm não, vành đai và bó dọc trên. KẾT LUẬN Các phát hiện của chúng tôi mở rộng thêm phổ di truyền của các đột biến SACS và mở rộng nghiên cứu về kiểu hình lâm sàng. Các đặc điểm của MRI chỉ ra rằng những thay đổi ở cầu não và bất thường trên lều là chẩn đoán. Sự biểu diễn quá mức của TPF trên DTI gợi ý rằng có thành phần phát triển trong quá trình sinh bệnh của bệnh."} +{"idx": 1980143, "en": "OBJECTIVE To estimate the incremental effects of transitions in electronic health record (EHR) system capabilities on hospital process quality. DATA SOURCE Hospital Compare (process quality), Health Information and Management Systems Society Analytics (EHR use), and Inpatient Prospective Payment System (hospital characteristics) for 2006-2010. STUDY SETTING Hospital EHR systems were categorized into five levels (Level_0 to Level_4) based on use of eight clinical applications. Level_3 systems can meet 2011 EHR \"meaningful use\" objectives. Process quality was measured as composite scores on a 100-point scale for heart attack, heart failure, pneumonia, and surgical care infection prevention. Statistical analyses were conducted using fixed effects linear panel regression model for all hospitals, hospitals stratified on condition-specific baseline quality, and for large hospitals. PRINCIPAL FINDINGS Among all hospitals, implementing Level_3 systems yielded an incremental 0.35-0.49 percentage point increase in quality (over Level_2) across three conditions. Hospitals in bottom quartile of baseline quality increased 1.16-1.61 percentage points across three conditions for reaching Level_3. However, transitioning to Level_4 yielded an incremental decrease of 0.90-1.0 points for three conditions among all hospitals and 0.65-1.78 for bottom quartile hospitals. CONCLUSIONS Hospitals transitioning to EHR systems capable of meeting 2011 meaningful use objectives improved process quality, and lower quality hospitals experienced even higher gains. However, hospitals that transitioned to more advanced systems saw quality declines.", "gemini": "MỤC TIÊU Ước tính các tác động gia tăng của quá trình chuyển đổi trong các khả năng của hệ thống hồ sơ sức khỏe điện tử (EHR) đối với chất lượng quy trình tại bệnh viện. NGUỒN DỮ LIỆU So sánh bệnh viện (chất lượng quy trình), Hệ thống phân tích của Hiệp hội quản lý và thông tin y tế (sử dụng EHR) và Hệ thống thanh toán tiền trả cho bệnh nhân nội trú (đặc điểm bệnh viện) trong giai đoạn 2006-2010. CÀI ĐẶT NGHIÊN CỨU Các hệ thống EHR của bệnh viện được phân loại thành năm cấp độ (Cấp độ_0 đến Cấp độ_4) dựa trên việc sử dụng tám ứng dụng lâm sàng. Các hệ thống cấp độ_3 có thể đáp ứng các mục tiêu \"sử dụng có ý nghĩa\" của EHR năm 2011. Chất lượng quy trình được đo bằng điểm tổng hợp trên thang điểm 100 cho bệnh tim, suy tim, viêm phổi và phòng ngừa nhiễm trùng chăm sóc phẫu thuật. Các phân tích thống kê được tiến hành bằng cách sử dụng mô hình hồi quy tuyến tính hiệu ứng cố định cho tất cả các bệnh viện, các bệnh viện được phân tầng theo chất lượng cơ sở cụ thể theo từng tình trạng và đối với các bệnh viện lớn. KẾT LUẬN CHÍNH Trong số tất cả các bệnh viện, việc triển khai các hệ thống Cấp độ_3 đã mang lại mức tăng gia tăng 0,35-0,49 phần trăm về chất lượng (so với Cấp độ_2) trên ba tình trạng. Các bệnh viện trong tứ phân vị cuối của chất lượng cơ sở đã tăng 1,16-1,61 phần trăm trên ba tình trạng để đạt đến Cấp độ_3. Tuy nhiên, việc chuyển sang Cấp độ_4 đã dẫn đến mức giảm gia tăng 0,90-1,0 điểm cho ba tình trạng trong số tất cả các bệnh viện và 0,65-1,78 đối với các bệnh viện tứ phân vị cuối. KẾT LUẬN Các bệnh viện chuyển sang các hệ thống EHR có khả năng đáp ứng các mục tiêu sử dụng có ý nghĩa của năm 2011 đã cải thiện chất lượng quy trình và các bệnh viện có chất lượng thấp hơn thậm chí còn đạt được mức tăng cao hơn. Tuy nhiên, các bệnh viện chuyển sang các hệ thống tiên tiến hơn đã chứng kiến chất lượng suy giảm."} diff --git a/retranslated_vi_pubmed/chunk_198/error.json b/retranslated_vi_pubmed/chunk_198/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_198/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_199/completed.json b/retranslated_vi_pubmed/chunk_199/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..c07ab20a12bd3ea242d4e5029c6b45feabddf7a0 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_199/completed.json @@ -0,0 +1,143 @@ +[ + 1990000, + 1990001, + 1990002, + 1990003, + 1990004, + 1990005, + 1990006, + 1990007, + 1990008, + 1990009, + 1990010, + 1990011, + 1990012, + 1990013, + 1990014, + 1990015, + 1990016, + 1990017, + 1990018, + 1990019, + 1990020, + 1990021, + 1990022, + 1990023, + 1990024, + 1990025, + 1990026, + 1990027, + 1990028, + 1990029, + 1990030, + 1990031, + 1990032, + 1990033, + 1990034, + 1990035, + 1990036, + 1990037, + 1990038, + 1990039, + 1990040, + 1990041, + 1990042, + 1990043, + 1990044, + 1990045, + 1990046, + 1990047, + 1990048, + 1990049, + 1990050, + 1990051, + 1990052, + 1990053, + 1990054, + 1990055, + 1990056, + 1990057, + 1990058, + 1990059, + 1990060, + 1990061, + 1990062, + 1990063, + 1990064, + 1990065, + 1990066, + 1990067, + 1990068, + 1990069, + 1990070, + 1990071, + 1990072, + 1990073, + 1990074, + 1990075, + 1990076, + 1990077, + 1990078, + 1990079, + 1990080, + 1990081, + 1990082, + 1990083, + 1990084, + 1990085, + 1990086, + 1990087, + 1990088, + 1990089, + 1990090, + 1990091, + 1990092, + 1990093, + 1990094, + 1990095, + 1990096, + 1990097, + 1990098, + 1990102, + 1990103, + 1990104, + 1990105, + 1990106, + 1990107, + 1990108, + 1990109, + 1990110, + 1990111, + 1990112, + 1990113, + 1990114, + 1990115, + 1990116, + 1990117, + 1990118, + 1990119, + 1990120, + 1990121, + 1990122, + 1990123, + 1990124, + 1990125, + 1990126, + 1990127, + 1990128, + 1990129, + 1990130, + 1990131, + 1990132, + 1990133, + 1990134, + 1990135, + 1990136, + 1990137, + 1990138, + 1990139, + 1990140, + 1990141, + 1990142, + 1990143 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_199/data.jsonl b/retranslated_vi_pubmed/chunk_199/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..e7121a4cc04700cd478689be7abe5d668be845f2 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_199/data.jsonl @@ -0,0 +1,141 @@ +{"idx": 1990000, "en": "How can physical systems of the brain, explain a psychological phenomenon such as personality? Personality is an emergent property of the brain as such it requires interacting elements that generate a whole. Per definition a physical system is a compound whole made of interacting interdependent elements. The brain is composed of multiple levels of elements ranging from single neurons interconnected by axons dendrites and synapses, up to brain regions and neural network ensembles connected by multiple modalities, from direct physical pathways to synchronized functional connectivity. Today we know that the brain develops and wires according to the influences of its environment, this is called \"experience dependent plasticity\" and follows Hebbian-like algorithms. Such process \"embeds\" into the brain internal representations in the form of physical attractor configurations distributed within the brain neural-networks. Development entails formation of personal individual-specific network configurations found recently as resting-state networks or \"default-mode networks.\" These internal configurations represent the outer world to us and determine the way we perceive it and react to it. In other words these internal configurations determine our personality styles. The internal representations continuously adapt to the changing worlds offering good adaptability and effective functionality in our changing environments. Personality disorders are reconceptualized in terms of altered disturbed mal-developed default-mode-networks, such that the internal representations are biased, limited, fixated and non-adaptive. In this context therapy of personality disorders can be reconceptualized as experience-depended plasticity therapy.", "gemini": "Làm thế nào mà các hệ thống vật lý của não bộ có thể giải thích được một hiện tượng tâm lý như tính cách? Tính cách là một đặc tính mới nổi của não bộ vì vậy nó đòi hỏi các yếu tố tương tác tạo ra một tổng thể. Theo định nghĩa, một hệ thống vật lý là một tổng thể phức hợp được tạo thành từ các yếu tố phụ thuộc lẫn nhau. Bộ não bao gồm nhiều cấp độ các yếu tố, từ các tế bào thần kinh đơn lẻ được kết nối với nhau bằng các sợi trục, các sợi nhánh và các khớp thần kinh, cho đến các vùng não và các nhóm mạng lưới thần kinh được kết nối với nhau bằng nhiều phương thức, từ các đường dẫn vật lý trực tiếp đến kết nối chức năng được đồng bộ hóa. Ngày nay chúng ta biết rằng não bộ phát triển và kết nối theo những ảnh hưởng của môi trường, điều này được gọi là \"tính dẻo theo kinh nghiệm\" và tuân theo các thuật toán giống như Hebbian. Quá trình như vậy \"nhúng\" vào não các biểu diễn bên trong dưới dạng các cấu hình hấp dẫn vật lý được phân bố trong các mạng lưới thần kinh của não. Sự phát triển đòi hỏi sự hình thành các cấu hình mạng lưới riêng biệt cho từng cá nhân, được tìm thấy gần đây là các mạng lưới trạng thái nghỉ hoặc \"mạng lưới chế độ mặc định\". Các cấu hình bên trong này đại diện cho thế giới bên ngoài đối với chúng ta và xác định cách chúng ta nhận thức và phản ứng với thế giới đó. Nói cách khác, những cấu hình bên trong này xác định phong cách tính cách của chúng ta. Các biểu diễn bên trong liên tục thích ứng với thế giới thay đổi, mang lại khả năng thích ứng tốt và chức năng hiệu quả trong môi trường thay đổi của chúng ta. Các rối loạn nhân cách được định nghĩa lại theo nghĩa là các mạng lưới chế độ mặc định bị thay đổi, rối loạn, phát triển kém, do đó các biểu diễn bên trong bị thiên lệch, hạn chế, cố định và không thích ứng. Trong bối cảnh này, liệu pháp về các rối loạn nhân cách có thể được định nghĩa lại là liệu pháp dẻo theo kinh nghiệm."} +{"idx": 1990001, "en": "The mental representation of one's own body does not necessarily correspond to the physical body. For instance, a dissociation between perceived and actual reach-ability has been shown, that is, individuals perceive that they can reach objects that are out of grasp. We presented participants with 3D pictures of objects located at four different distances, namely near-reaching space, actual-reaching space, perceived-reaching space and non-reaching space. Immediately after they were presented with function, manipulation, observation or pointing verbs and were required to judge if the verb was compatible with the object. Participants were faster with function and manipulation verbs than with observation and pointing verbs. Strikingly, with both function and manipulation verbs participants were faster when objects were presented in actual than the perceived reaching space. These findings suggest that our knowledge of the world is implicitly built online through behaviour, and is not necessarily reflected in explicit estimates or conscious representations.", "gemini": "Biểu diễn tinh thần về cơ thể của chính mình không nhất thiết phải tương ứng với cơ thể vật lý. Ví dụ, sự tách biệt giữa khả năng với được và thực tế với được đã được chứng minh, tức là các cá nhân nhận thấy rằng họ có thể với tới các vật thể nằm ngoài tầm với. Chúng tôi đã trình bày cho những người tham gia các bức ảnh 3D về các vật thể được đặt ở bốn khoảng cách khác nhau, cụ thể là không gian với gần, không gian với thực tế, không gian với được và không gian không với được. Ngay sau khi họ được trình bày với chức năng, thao tác, quan sát hoặc động từ chỉ, họ được yêu cầu đánh giá xem động từ đó có tương thích với vật thể hay không. Những người tham gia nhanh hơn với các động từ chức năng và thao tác so với các động từ quan sát và chỉ. Đáng ngạc nhiên là với cả động từ chức năng và thao tác, người tham gia nhanh hơn khi các vật thể được trình bày ở không gian với thực tế hơn là không gian với được. Những phát hiện này cho thấy rằng kiến thức của chúng ta về thế giới được xây dựng trực tuyến một cách ngầm định thông qua hành vi và không nhất thiết được phản ánh trong các ước tính rõ ràng hoặc các biểu diễn có ý thức."} +{"idx": 1990002, "en": "Recent studies have shown the importance of integrating multisensory information in the body representation for constituting self-consciousness. However, one idea that has received only scant attention is that our body representation is also constituted by knowledge of bodily visual characteristics (i.e. 'what I look like'). Here in two experiments we used a full body crossmodal congruency task in which visual distractors were presented on a photograph of the participant, another person, who was either familiar or unfamiliar, or an object. Results revealed that during the 'self-condition' CCEs were enhanced compared to the 'other condition'. The CCE was similar for unfamiliar and familiar others. CCEs for the object condition were significantly smaller. The results show that presentation of an irrelevant image of a body affects multimodal processing and that the effect is enhanced when that image is of the self. The results hold intriguing implications for body representation in social situations.", "gemini": "Các nghiên cứu gần đây đã chỉ ra tầm quan trọng của việc tích hợp thông tin đa giác quan trong biểu diễn cơ thể để tạo nên ý thức về bản thân. Tuy nhiên, một ý tưởng chỉ nhận được ít sự chú ý là biểu diễn cơ thể của chúng ta cũng được tạo nên bởi kiến thức về các đặc điểm thị giác của cơ thể (tức là 'tôi trông như thế nào'). Ở đây trong hai thí nghiệm, chúng tôi đã sử dụng một nhiệm vụ phù hợp đa phương thức toàn thân trong đó các yếu tố gây nhiễu thị giác được trình bày trên một bức ảnh của người tham gia, một người khác, người quen hoặc không quen biết hoặc một vật thể. Kết quả cho thấy rằng trong 'điều kiện tự thân' CCE được tăng cường so với 'điều kiện khác'. CCE tương tự nhau đối với những người khác quen thuộc và không quen thuộc. CCE đối với điều kiện đối tượng nhỏ hơn đáng kể. Kết quả cho thấy rằng việc trình bày một hình ảnh không liên quan của cơ thể ảnh hưởng đến quá trình xử lý đa phương thức và hiệu ứng này được tăng cường khi hình ảnh đó là của chính bản thân người đó. Kết quả có những hàm ý hấp dẫn đối với biểu diễn cơ thể trong các tình huống xã hội."} +{"idx": 1990003, "en": "Gadolinium-based nanoparticles are novel objects with interesting physical properties, allowing their use for diagnostic and therapeutic applications. Gadolinium-based nanoparticles were imaged following intravenous injection in healthy rats and rats grafted with 9L gliosarcoma tumors using magnetic resonance imaging and scintigraphic imaging. Quantitative biodistribution using gamma-counting of each sampled organ confirmed that these nanoparticles were rapidly cleared essentially by renal excretion. Accumulation of these nanoparticles in 9L gliosarcoma tumors implanted in the rat brain was quantitated. This passive and long-duration accumulation of gadolinium-based nanoparticles in tumor, which is related to disruption of the blood-brain barrier, is in good agreement with the use of these nanoparticles as radiosensitizers for brain tumors.", "gemini": "Các hạt nano gốc gadolinium là những vật thể mới lạ có các tính chất vật lý thú vị, cho phép sử dụng chúng cho các ứng dụng chẩn đoán và điều trị. Các hạt nano gốc gadolinium đã được chụp ảnh sau khi tiêm tĩnh mạch ở chuột khỏe mạnh và chuột được ghép khối u gliosarcoma 9L bằng cách sử dụng chụp cộng hưởng từ và chụp cắt lớp hạt nhân. Phân bố sinh học định lượng bằng cách đếm gamma của từng cơ quan được lấy mẫu đã xác nhận rằng các hạt nano này được đào thải nhanh chóng chủ yếu bằng cách bài tiết qua thận. Sự tích tụ của các hạt nano này trong các khối u gliosarcoma 9L được cấy ghép vào não chuột đã được định lượng. Sự tích tụ thụ động và kéo dài này của các hạt nano gốc gadolinium trong khối u, liên quan đến sự phá vỡ hàng rào máu não, phù hợp với việc sử dụng các hạt nano này làm chất phóng xạ cho khối u não."} +{"idx": 1990004, "en": "The failure to understand the mechanisms of biomaterial-associated thrombosis prevents us from improving the blood compatibility of stents and mechanical heart valves. Blood-material interactions trigger a complex series of events and anticoagulant and anti-platelet therapies are needed to reduce the risks of thrombotic complications with most cardiovascular materials. While material interaction with platelets has been widely studied, little is currently known on material-induced leukocyte activation in the presence of shear. In vitro experiments were performed to assess the effect of flow on blood cell activation induced by medical grade metals, ST316L and TiAl6V4. Blood was circulated in flow chambers preloaded with or without metal wires at shear rates of 100, 500, and 1500 s⁻¹. Platelet and leukocyte activation, leukocyte-platelet aggregation, and tissue factor expression on monocytes were measured by flow cytometry. Metal surfaces were characterized by scanning electron microscopy. Under physiological shear rates, no significant platelet microparticle formation was observed. However, significant CD11b up-regulation, leukocyte-platelet aggregates, and tissue factor expression were observed at 100 s⁻¹. As shear rate increased to 1500 s⁻¹, leukocyte activation reduced to control values. TiAl6V4-induced leukocyte activation was generally lower than that of ST316L. Adhesion significantly decreased with increasing shear rate to 1500 s⁻¹. In blood, increase within physiological shear rates led to a significant reduction in in vitro material-induced leukocyte activation, suggesting that difference between material biocompatibility may be better identified at low shear rates or under pathological shear conditions.", "gemini": "Việc không hiểu được các cơ chế của huyết khối liên quan đến vật liệu sinh học khiến chúng ta không thể cải thiện khả năng tương thích máu của stent và van tim cơ học. Các tương tác máu-vật liệu kích hoạt một loạt các sự kiện phức tạp và cần sử dụng liệu pháp chống đông máu và chống tiểu cầu để giảm nguy cơ biến chứng huyết khối với hầu hết các vật liệu tim mạch. Trong khi tương tác vật liệu với tiểu cầu đã được nghiên cứu rộng rãi, thì hiện tại người ta vẫn biết rất ít về hoạt hóa bạch cầu do vật liệu gây ra khi có sự trượt. Các thí nghiệm trong ống nghiệm đã được thực hiện để đánh giá tác động của dòng chảy đối với hoạt hóa tế bào máu do các kim loại cấp y tế, ST316L và TiAl6V4 gây ra. Máu được lưu thông trong các buồng dòng chảy được nạp trước với hoặc không có dây kim loại ở tốc độ trượt là 100, 500 và 1500 s⁻¹. Hoạt hóa tiểu cầu và bạch cầu, kết tập tiểu cầu-bạch cầu và biểu hiện yếu tố mô trên các tế bào đơn nhân được đo bằng phương pháp đo lưu lượng tế bào. Các bề mặt kim loại được đặc trưng bằng kính hiển vi điện tử quét. Theo tốc độ trượt sinh lý, không quan sát thấy sự hình thành tiểu phân tiểu cầu có ý nghĩa. Tuy nhiên, sự điều hòa tăng CD11b, các tập hợp tiểu cầu-bạch cầu và biểu hiện yếu tố mô đã được quan sát thấy ở mức 100 s⁻¹. Khi tốc độ trượt tăng lên 1500 s⁻¹, hoạt hóa bạch cầu giảm xuống các giá trị kiểm soát. Hoạt hóa bạch cầu do TiAl6V4 gây ra thường thấp hơn so với ST316L. Độ bám dính giảm đáng kể khi tăng tốc độ trượt lên 1500 s⁻¹. Trong máu, sự gia tăng trong phạm vi trượt sinh lý dẫn đến giảm đáng kể hoạt hóa bạch cầu do vật liệu trong ống nghiệm, cho thấy rằng sự khác biệt giữa khả năng tương thích sinh học của vật liệu có thể được xác định tốt hơn ở tốc độ trượt thấp hoặc trong điều kiện trượt bệnh lý."} +{"idx": 1990005, "en": "Protein polymer-based hydrogels have shown potential for tissue engineering applications, but require biocompatibility testing for in vivo use. Enzymatically crosslinked protein polymer-based hydrogels were tested in vitro and in vivo to evaluate their biocompatibility. Endotoxins present in the hydrogel were removed by Trition X-114 phase separation. The reduction of endotoxins decreased TNF-α production by a macrophage cell line in vitro; however, significant inflammatory response was still present compared to collagen control gels. A branched PEG molecule and dexamethasone were added to the hydrogel to reduce the response. In vitro testing showed a decrease in the TNF-α levels with the addition of dexamethasone. In vivo implantations into the epididymal fat pad of C57/BL6 mice, however, indicated a decreased inflammatory mediated immune response with a hydrogel treated with both PEGylation and endotoxin reduction. This study demonstrates the importance of endotoxin testing and removal in determining the biocompatibility of biomaterials.", "gemini": "Các hydrogel dựa trên polyme protein đã cho thấy tiềm năng ứng dụng trong kỹ thuật mô, nhưng cần phải kiểm tra khả năng tương thích sinh học để sử dụng trong cơ thể sống. Các hydrogel dựa trên polyme protein liên kết chéo bằng enzym đã được thử nghiệm trong ống nghiệm và trong cơ thể sống để đánh giá khả năng tương thích sinh học của chúng. Các nội độc tố có trong hydrogel đã được loại bỏ bằng phương pháp tách pha Trition X-114. Việc giảm nội độc tố làm giảm sản xuất TNF-α của dòng tế bào đại thực bào trong ống nghiệm; tuy nhiên, đáp ứng viêm đáng kể vẫn có so với gel collagen đối chứng. Một phân tử PEG phân nhánh và dexamethasone đã được thêm vào hydrogel để giảm đáp ứng. Kiểm tra trong ống nghiệm cho thấy mức TNF-α giảm khi bổ sung dexamethasone. Tuy nhiên, việc cấy ghép trong cơ thể sống vào lớp mỡ mào tinh hoàn của chuột C57/BL6 cho thấy đáp ứng miễn dịch do viêm giảm với hydrogel được xử lý bằng cả PEG hóa và giảm nội độc tố. Nghiên cứu này chứng minh tầm quan trọng của việc kiểm tra và loại bỏ nội độc tố trong việc xác định khả năng tương thích sinh học của vật liệu sinh học."} +{"idx": 1990006, "en": "Integrated fixed-film activated sludge systems (IFFAS) may achieve year-round nitrification or gain additional treatment capacity due to the presence of both flocs and biofilms, and the potential for multiple redox states and long solids retention time. Flocs and biofilms are distinctive microbial structures and characterization of the physicochemical and structural properties of these may provide insight into their respective roles in wastewater treatment and contaminant removal in IFFAS. Flocs and biofilms were examined from five different pilot media systems being evaluated for potential full scale implementation at a large municipal wastewater treatment plant. Flocs and biofilms within the same system possessed different surface characteristics; flocs were found to have a higher negative surface charge (-0.35 to -0.65 meq./g VSS) and are more hydrophobic (60%-75%) than biofilms (-0.05 to -0.07 meq/g VSS; 19-34%). The EPS content of flocs was significantly higher (range of 2.1-4.5 folds) than that of biofilms. In floc-derived extracellular polymeric substances (EPS), protein (PN) was clearly dominant; whereas in biofilm-derived EPS, PN and polysaccharide (PS) were present in approximately equal proportions. Biofilm EPS had a higher proportion of DNA when compared to flocs. Biofilm growth was preferential on the protected internal surfaces of the media. Colonization of the external surfaces of the media was evident by the presence of small microcolonies. The structural heterogeneity of the biofilms examined was supported by observed differences in biomass content, thickness and roughness of biofilm surface. The biofilm on the interior surface of media was found to be patchy with clusters of cells connected by an irregular arrangement of interconnecting EPS projections. Biofilm thickness ranged between 139 μm and 253 μm. The pattern of oxygen penetration is expected to be complex. Nitrifiers and denitrifiers were predominantly associated with the biofilms, and the latter were found to be dispersed throughout the film and arranged in micro-clusters, suggesting partial oxygen penetration.", "gemini": "Các hệ thống bùn hoạt tính màng cố định tích hợp (IFFAS) có thể đạt được quá trình nitrat hóa quanh năm hoặc tăng thêm khả năng xử lý do sự hiện diện của cả bông cặn và màng sinh học, và khả năng có nhiều trạng thái oxy hóa khử và thời gian giữ chất rắn lâu. Bông cặn và màng sinh học là các cấu trúc vi sinh vật đặc biệt và việc xác định các tính chất lý hóa và cấu trúc của chúng có thể cung cấp cái nhìn sâu sắc về vai trò tương ứng của chúng trong xử lý nước thải và loại bỏ chất gây ô nhiễm trong IFFAS. Bông cặn và màng sinh học đã được kiểm tra từ năm hệ thống phương tiện thí điểm khác nhau đang được đánh giá để triển khai toàn diện tiềm năng tại một nhà máy xử lý nước thải đô thị lớn. Bông cặn và màng sinh học trong cùng một hệ thống sở hữu các đặc tính bề mặt khác nhau; bông cặn được phát hiện có điện tích bề mặt âm cao hơn (-0,35 đến -0,65 meq./g VSS) và kỵ nước hơn (60%-75%) so với màng sinh học (-0,05 đến -0,07 meq/g VSS; 19-34%). Hàm lượng EPS của bông cặn cao hơn đáng kể (trong khoảng 2,1-4,5 lần) so với màng sinh học. Trong các chất ngoại bào có nguồn gốc từ bông cặn (EPS), protein (PN) chiếm ưu thế rõ ràng; trong khi trong EPS có nguồn gốc từ màng sinh học, PN và polysaccharide (PS) có tỷ lệ gần bằng nhau. EPS màng sinh học có tỷ lệ DNA cao hơn so với bông cặn. Sự phát triển của màng sinh học được ưu tiên trên các bề mặt bên trong được bảo vệ của phương tiện. Sự xâm chiếm các bề mặt bên ngoài của phương tiện được thể hiện bằng sự hiện diện của các vi khuẩn lạc nhỏ. Tính không đồng nhất về cấu trúc của các màng sinh học được kiểm tra được hỗ trợ bởi sự khác biệt quan sát được về hàm lượng sinh khối, độ dày và độ nhám của bề mặt màng sinh học. Màng sinh học trên bề mặt bên trong của phương tiện được phát hiện là không đồng đều với các cụm tế bào được kết nối bởi một sự sắp xếp không đều của các phần nhô EPS kết nối. Độ dày của màng sinh học dao động trong khoảng từ 139 μm đến 253 μm. Mô hình thâm nhập oxy dự kiến sẽ phức tạp. Các vi khuẩn nitrat hóa và vi khuẩn khử nitrat chủ yếu liên quan đến màng sinh học và các vi khuẩn sau được phát hiện phân tán khắp màng và sắp xếp thành các vi cụm, cho thấy sự thâm nhập oxy một phần."} +{"idx": 1990007, "en": "Lung transplant recipients (LTRs) are prone to medical complications and emergencies due to the transplanted organ being in constant direct contact with the environment and the need for life-long profound immunosuppression (IS). As a result of these specific circumstances, the medical and surgical management of LTRs frequently differs from usual standard care. Therefore, we outline here some of the principles we take into account when dealing with the most frequent medical emergencies encountered in our lung transplant cohort in Zurich. The main topics dealt with are: diagnostics and treatment of infections, gastrointestinal emergencies, IS and other medication issues as well as work-up of unclear inflammatory signs and peri-operative precautions in LTRs. Early post-operative transplant complications, rare medical emergencies and surgical problems are not covered. Our report is intended to help internists and pulmonologists new to the field to obtain a better understanding of the peculiarities of LTRs and their management.", "gemini": "Những người nhận ghép phổi (LTR) dễ gặp các biến chứng y tế và trường hợp cấp cứu do cơ quan được ghép liên tục tiếp xúc trực tiếp với môi trường và cần phải ức chế miễn dịch sâu suốt đời (IS). Do những hoàn cảnh cụ thể này, việc quản lý y tế và phẫu thuật của LTR thường khác với cách chăm sóc tiêu chuẩn thông thường. Do đó, chúng tôi phác thảo ở đây một số nguyên tắc mà chúng tôi tính đến khi xử lý các trường hợp cấp cứu y tế thường gặp nhất trong nhóm ghép phổi của chúng tôi tại Zurich. Các chủ đề chính được đề cập là: chẩn đoán và điều trị nhiễm trùng, các trường hợp cấp cứu về đường tiêu hóa, IS và các vấn đề về thuốc khác cũng như giải quyết các dấu hiệu viêm không rõ ràng và các biện pháp phòng ngừa trước và trong phẫu thuật ở LTR. Các biến chứng ghép sau phẫu thuật sớm, các trường hợp cấp cứu y tế hiếm gặp và các vấn đề phẫu thuật không được đề cập. Báo cáo của chúng tôi nhằm giúp các bác sĩ nội khoa và bác sĩ chuyên khoa phổi mới vào nghề hiểu rõ hơn về những đặc điểm riêng của LTR và cách quản lý của họ."} +{"idx": 1990008, "en": "An increase in the number of AMPA-type glutamate receptors (AMPARs) is critical for long-term potentiation (LTP), synaptic plasticity regarded as a basal mechanism of learning and memory. However, when and how each type of AMPAR reaches the postsynaptic membrane remain unclear. We have developed experimental methods to form postsynaptic-like membrane (PSLM) on a glass surface to precisely visualize the location and movement of receptors. We observed fluorescence-labeled AMPAR subunits (GluA1-3) around PSLM with total internal reflection fluorescence microscopy. The increases of GluA1, 2, and 3 in PSLM showed different time courses after LTP induction. GluA1 increased first, and was exocytosed to the periphery of PSLM soon after LTP induction. GluA2 and GluA3 initially decreased, and then increased. Exocytosis of GluA2 and GluA3 occurred primarily in non-PSLM, and later than exocytosis of GluA1. Thus, GluA1-3 appear to increase in the postsynaptic membrane through distinct pathways during LTP.", "gemini": "Sự gia tăng số lượng thụ thể glutamate loại AMPA (AMPAR) rất quan trọng đối với sự tăng cường lâu dài (LTP), tính dẻo của khớp thần kinh được coi là cơ chế cơ bản của việc học và trí nhớ. Tuy nhiên, thời điểm và cách thức từng loại AMPAR tiếp cận màng sau synap vẫn chưa rõ ràng. Chúng tôi đã phát triển các phương pháp thực nghiệm để tạo màng giống màng sau synap (PSLM) trên bề mặt thủy tinh để hình dung chính xác vị trí và chuyển động của các thụ thể. Chúng tôi quan sát thấy các tiểu đơn vị AMPAR được gắn huỳnh quang (GluA1-3) xung quanh PSLM bằng kính hiển vi huỳnh quang phản xạ toàn phần bên trong. Sự gia tăng của GluA1, 2 và 3 trong PSLM cho thấy các quá trình thời gian khác nhau sau khi gây LTP. GluA1 tăng đầu tiên, và được xuất bào đến ngoại vi của PSLM ngay sau khi gây LTP. GluA2 và GluA3 ban đầu giảm, sau đó tăng. Sự xuất bào của GluA2 và GluA3 xảy ra chủ yếu ở ngoài PSLM và muộn hơn so với sự xuất bào của GluA1. Do đó, GluA1-3 dường như tăng lên ở màng sau synap thông qua các con đường riêng biệt trong quá trình LTP."} +{"idx": 1990009, "en": "Chromosomal instability is a key feature in cancer progression. Recently we have reported that BRCA1 regulates the transcription of several genes in prostate cancer, including ATM (ataxia telangiectasia mutated). Although it is well accepted that ATM is a pivotal mediator in genotoxic stress, it is unknown whether ATM transcription is regulated during the molecular response to DNA damage. Here we investigate ATM transcription regulation in human prostate tumor PC3 cell line. We have found that doxorubicin and mitoxantrone repress ATM transcription in PC3 cells but etoposide and methotrexate do not affect ATM expression. We have demonstrated that BRCA1 binds to ATM promoter and after doxorubicin exposure, it is released. BRCA1 overexpression increases ATM transcription and this enhancement is abolished by BRCA1 depletion. Moreover, BRCA1-BRCT domain loss impairs the ability of BRCA1 to regulate ATM promoter activity, strongly suggesting that BRCT domain is essential for ATM regulation by BRCA1. BRCA1-overexpressing PC3 cells exposed to KU55933 ATM kinase inhibitor showed significant decreased ATM promoter activity compared to untreated cells, suggesting that ATM transcriptional regulation by BRCA1 is partially mediated by the ATM kinase activity. In addition, we have demonstrated E2F1 binding to ATM promoter before and after doxorubicin exposure. E2F1 overexpression diminishes ATM transcription after doxorubicin exposure which is impaired by E2F1 dominant negative mutants. Finally, the co-regulator of transcription CtIP increases ATM transcription. CtIP increases ATM transcription. Altogether, BRCA1/E2F1/CtIP binding to ATM promoter activates ATM transcription. Doxorubicin exposure releases BRCA1 and CtIP from ATM promoter still keeping E2F1 recruited and, in turn, represses ATM expression.", "gemini": "Sự bất ổn nhiễm sắc thể là một đặc điểm chính trong tiến triển ung thư. Gần đây, chúng tôi đã báo cáo rằng BRCA1 điều chỉnh phiên mã của một số gen trong ung thư tuyến tiền liệt, bao gồm ATM (ataxia telangiectasia đột biến). Mặc dù người ta đã chấp nhận rằng ATM là một trung gian chính trong stress gen độc, nhưng không biết liệu phiên mã ATM có được điều chỉnh trong quá trình đáp ứng phân tử với tổn thương DNA hay không. Ở đây, chúng tôi điều tra quá trình điều chỉnh phiên mã ATM trong dòng tế bào u tuyến tiền liệt PC3 của người. Chúng tôi đã phát hiện ra rằng doxorubicin và mitoxantrone ức chế phiên mã ATM trong các tế bào PC3 nhưng etoposide và methotrexate không ảnh hưởng đến biểu hiện ATM. Chúng tôi đã chứng minh rằng BRCA1 liên kết với vùng khởi động ATM và sau khi tiếp xúc với doxorubicin, nó sẽ được giải phóng. Sự biểu hiện quá mức của BRCA1 làm tăng phiên mã ATM và sự tăng cường này bị loại bỏ do sự suy giảm BRCA1. Hơn nữa, mất miền BRCA1-BRCT làm suy yếu khả năng điều chỉnh hoạt động của vùng khởi động ATM của BRCA1, cho thấy mạnh mẽ rằng miền BRCT rất cần thiết cho quá trình điều chỉnh ATM của BRCA1. Các tế bào PC3 biểu hiện quá mức BRCA1 tiếp xúc với chất ức chế kinase ATM KU55933 cho thấy hoạt động của vùng khởi động ATM giảm đáng kể so với các tế bào chưa được xử lý, cho thấy rằng quá trình điều chỉnh phiên mã ATM của BRCA1 được trung gian một phần bởi hoạt động của kinase ATM. Ngoài ra, chúng tôi đã chứng minh sự liên kết của E2F1 với vùng khởi động ATM trước và sau khi tiếp xúc với doxorubicin. Sự biểu hiện quá mức của E2F1 làm giảm phiên mã ATM sau khi tiếp xúc với doxorubicin bị suy yếu bởi các đột biến âm tính trội của E2F1. Cuối cùng, chất đồng điều chỉnh phiên mã CtIP làm tăng phiên mã ATM. CtIP làm tăng phiên mã ATM. Nhìn chung, sự liên kết của BRCA1/E2F1/CtIP với vùng khởi động ATM sẽ kích hoạt phiên mã ATM. Tiếp xúc với doxorubicin giải phóng BRCA1 và CtIP khỏi vùng khởi động ATM vẫn giữ E2F1 được tuyển dụng và do đó, ức chế biểu hiện ATM."} +{"idx": 1990010, "en": "Cohesion is essential for the identification of sister chromatids and for the biorientation of chromosomes until their segregation. Here, we have demonstrated that an RNA-binding motif protein encoded on the X chromosome (RBMX) plays an essential role in chromosome morphogenesis through its association with chromatin, but not with RNA. Depletion of RBMX by RNA interference (RNAi) causes the loss of cohesin from the centromeric regions before anaphase, resulting in premature chromatid separation accompanied by delocalization of the shugoshin complex and outer kinetochore proteins. Cohesion defects caused by RBMX depletion can be detected as early as the G2 phase. Moreover, RBMX associates with the cohesin subunits, Scc1 and Smc3, and with the cohesion regulator, Wapl. RBMX is required for cohesion only in the presence of Wapl, suggesting that RBMX is an inhibitor of Wapl. We propose that RBMX is a cohesion regulator that maintains the proper cohesion of sister chromatids.", "gemini": "Sự gắn kết là rất cần thiết để nhận dạng các nhiễm sắc thể chị em và để định hướng sinh học của nhiễm sắc thể cho đến khi chúng phân ly. Ở đây, chúng tôi đã chứng minh rằng một protein có chủ đề liên kết RNA được mã hóa trên nhiễm sắc thể X (RBMX) đóng vai trò thiết yếu trong quá trình hình thái nhiễm sắc thể thông qua sự liên kết của nó với chromatin, nhưng không phải với RNA. Sự suy giảm RBMX do sự can thiệp của RNA (RNAi) gây ra sự mất đi của cohesin khỏi các vùng tâm động trước kỳ sau, dẫn đến sự tách biệt sớm của nhiễm sắc thể đi kèm với sự dịch chuyển của phức hợp shugoshin và các protein động học bên ngoài. Các khuyết tật gắn kết do sự suy giảm RBMX có thể được phát hiện sớm nhất là ở pha G2. Hơn nữa, RBMX liên kết với các tiểu đơn vị cohesin, Scc1 và Smc3, và với bộ điều chỉnh gắn kết, Wapl. RBMX chỉ cần thiết cho sự gắn kết khi có Wapl, cho thấy rằng RBMX là chất ức chế Wapl. Chúng tôi đề xuất rằng RBMX là một bộ điều chỉnh gắn kết duy trì sự gắn kết thích hợp của các nhiễm sắc thể chị em."} +{"idx": 1990011, "en": "Inhibition of NEDD8-activating enzyme (NAE) has emerged as a highly promising approach to treat cancer through the adenosine sulfamate analog MLN4924. Here, we show that selective pressure results in HCT116 colorectal carcinoma cells with decreased MLN4924 sensitivity and identify a single-nucleotide transition that changes alanine 171 to threonine (A171T) of the NAE subunit UBA3. This reduces the enzyme's affinity for MLN4924 and ATP while increasing NEDD8 activation at physiological ATP concentrations. Expression of UBA3 A171T is sufficient to decrease MLN4924 sensitivity of naive HCT116 cells, indicating that it is a dominant suppressor of MLN4924-mediated cell death. Our data suggest that the on-target potency of MLN4924 selects for a point mutation in NAE that overcomes the molecule's inhibitory effects, allowing cancer cell survival.", "gemini": "Việc ức chế enzyme hoạt hóa NEDD8 (NAE) đã nổi lên như một phương pháp rất hứa hẹn để điều trị ung thư thông qua chất tương tự adenosine sulfamate MLN4924. Ở đây, chúng tôi chỉ ra rằng áp lực chọn lọc dẫn đến các tế bào ung thư biểu mô đại trực tràng HCT116 có độ nhạy với MLN4924 giảm và xác định một sự chuyển đổi nucleotide đơn thay đổi alanine 171 thành threonine (A171T) của tiểu đơn vị NAE UBA3. Điều này làm giảm ái lực của enzyme đối với MLN4924 và ATP trong khi tăng hoạt hóa NEDD8 ở nồng độ ATP sinh lý. Biểu hiện của UBA3 A171T đủ để làm giảm độ nhạy với MLN4924 của các tế bào HCT116 ngây thơ, chỉ ra rằng đó là chất ức chế trội của quá trình chết tế bào do MLN4924 trung gian. Dữ liệu của chúng tôi cho thấy rằng hiệu lực trên đích của MLN4924 chọn lọc một đột biến điểm trong NAE khắc phục được các tác dụng ức chế của phân tử, cho phép tế bào ung thư sống sót."} +{"idx": 1990012, "en": "Microglia become activated by multiple types of damage in the nervous system and play essential roles in neuronal pathologies. However, how microglia transform into reactive phenotypes is poorly understood. Here, we identify the transcription factor interferon regulatory factor 8 (IRF8) as a critical regulator of reactive microglia. Within the spinal cord, IRF8 expression was normally low; however, the expression was markedly upregulated in microglia, but not in neurons or astrocytes, after peripheral nerve injury (PNI). IRF8 overexpression in cultured microglia promoted the transcription of genes associated with reactive states; conversely, IRF8 deficiency prevented these gene expressions in the spinal cord following PNI. Furthermore, IRF8-deficient mice were resistant to neuropathic pain, a common sequela of PNI, and transferring IRF8-overexpressing microglia spinally to normal mice produced pain. Therefore, IRF8 may activate a program of gene expression that transforms microglia into a reactive phenotype. Our findings provide a newly observed mechanism for microglial activation.", "gemini": "Vi glia hoạt hóa bởi nhiều loại tổn thương trong hệ thần kinh và đóng vai trò thiết yếu trong các bệnh lý thần kinh. Tuy nhiên, cách vi glia chuyển đổi thành kiểu hình phản ứng vẫn chưa được hiểu rõ. Ở đây, chúng tôi xác định yếu tố phiên mã interferon điều hòa yếu tố 8 (IRF8) là một chất điều hòa quan trọng của vi glia phản ứng. Trong tủy sống, biểu hiện IRF8 thường thấp; tuy nhiên, biểu hiện này tăng rõ rệt ở vi glia, nhưng không phải ở tế bào thần kinh hoặc tế bào hình sao, sau khi tổn thương dây thần kinh ngoại biên (PNI). Sự biểu hiện quá mức của IRF8 trong vi glia nuôi cấy thúc đẩy phiên mã các gen liên quan đến trạng thái phản ứng; ngược lại, sự thiếu hụt IRF8 ngăn chặn các biểu hiện gen này ở tủy sống sau PNI. Hơn nữa, những con chuột thiếu IRF8 có khả năng chống lại cơn đau thần kinh, một di chứng phổ biến của PNI, và việc truyền vi glia biểu hiện quá mức IRF8 vào tủy sống cho những con chuột bình thường gây ra đau. Do đó, IRF8 có thể kích hoạt một chương trình biểu hiện gen biến đổi vi glia thành kiểu hình phản ứng. Những phát hiện của chúng tôi cung cấp một cơ chế mới được quan sát thấy cho quá trình hoạt hóa vi glia."} +{"idx": 1990013, "en": "The synthesis of nucleotides in the body is centrally controlled by the liver, via salvage or de novo synthesis. We reveal a pervasive circadian influence on hepatic nucleotide metabolism, from rhythmic gene expression of rate-limiting enzymes to oscillating nucleotide metabolome in wild-type (WT) mice. Genetic disruption of the hepatic clock leads to aberrant expression of these enzymes, together with anomalous nucleotide rhythms, such as constant low levels of ATP with an excess in uric acid, the degradation product of purines. These results clearly demonstrate that the hepatic circadian clock orchestrates nucleotide synthesis and degradation. This circadian metabolome timetable, obtained using state-of-the-art capillary electrophoresis time-of-flight mass spectrometry, will guide further investigations in nucleotide metabolism-related disorders.", "gemini": "Việc tổng hợp các nucleotide trong cơ thể được kiểm soát tập trung bởi gan, thông qua quá trình cứu hộ hoặc tổng hợp de novo. Chúng tôi tiết lộ một ảnh hưởng phổ biến của nhịp sinh học đến quá trình chuyển hóa nucleotide ở gan, từ biểu hiện gen nhịp nhàng của các enzyme giới hạn tốc độ đến quá trình chuyển hóa nucleotide dao động ở chuột kiểu hoang dã (WT). Sự gián đoạn di truyền của đồng hồ gan dẫn đến biểu hiện bất thường của các enzyme này, cùng với nhịp nucleotide bất thường, chẳng hạn như mức ATP thấp liên tục với lượng dư axit uric, sản phẩm phân hủy của purin. Những kết quả này chứng minh rõ ràng rằng đồng hồ sinh học ở gan điều phối quá trình tổng hợp và phân hủy nucleotide. Bảng thời gian chuyển hóa nhịp sinh học này, thu được bằng cách sử dụng phổ khối thời gian bay điện di mao quản hiện đại, sẽ hướng dẫn các cuộc điều tra sâu hơn về các rối loạn liên quan đến quá trình chuyển hóa nucleotide."} +{"idx": 1990014, "en": "The PI3K-Akt-mTORC1 axis contributes to the activation, survival, and proliferation of CD4(+) T cells upon stimulation through TCR and CD28. Here, we demonstrate that the suppression of this axis by deletion of p85α or PI3K/mTORC1 inhibitors as well as T cell-specific deletion of raptor, an essential component of mTORC1, impairs Th17 differentiation in vitro and in vivo in a S6K1/2-dependent fashion. Inhibition of PI3K-Akt-mTORC1-S6K1 axis impairs the downregulation of Gfi1, a negative regulator of Th17 differentiation. Furthermore, we demonstrate that S6K2, a nuclear counterpart of S6K1, is induced by the PI3K-Akt-mTORC1 axis, binds RORγ, and carries RORγ to the nucleus. These results point toward a pivotal role of PI3K-Akt-mTORC1-S6K1/2 axis in Th17 differentiation.", "gemini": "Trục PI3K-Akt-mTORC1 góp phần vào quá trình hoạt hóa, tồn tại và tăng sinh của tế bào T CD4(+) khi được kích thích thông qua TCR và CD28. Ở đây, chúng tôi chứng minh rằng việc ức chế trục này bằng cách xóa p85α hoặc chất ức chế PI3K/mTORC1 cũng như xóa raptor đặc hiệu tế bào T, một thành phần thiết yếu của mTORC1, làm suy yếu sự biệt hóa Th17 trong ống nghiệm và trong cơ thể sống theo cách phụ thuộc vào S6K1/2. Việc ức chế trục PI3K-Akt-mTORC1-S6K1 làm suy yếu quá trình điều hòa giảm Gfi1, một chất điều hòa tiêu cực của sự biệt hóa Th17. Hơn nữa, chúng tôi chứng minh rằng S6K2, một đối tác hạt nhân của S6K1, được trục PI3K-Akt-mTORC1 cảm ứng, liên kết với RORγ và mang RORγ vào nhân. Những kết quả này chỉ ra vai trò quan trọng của trục PI3K-Akt-mTORC1-S6K1/2 trong sự biệt hóa Th17."} +{"idx": 1990015, "en": "Brains have to decide whether and how to respond to detected stimuli based on complex sensory input. The vinegar fly Drosophila melanogaster evaluates food sources based on olfactory cues. Here, we performed a behavioral screen using the vinegar fly and established the innate valence of 110 odorants. Our analysis of neuronal activation patterns evoked by attractive and aversive odorants suggests that even though the identity of odorants is coded by the set of activated receptors, the main representation of odorant valence is formed at the output level of the antennal lobe. The topographic clustering within the antennal lobe of valence-specific output neurons resembles a corresponding domain in the olfactory bulb of mice. The basal anatomical structure of the olfactory circuit between insects and vertebrates is known to be similar; our study suggests that the representation of odorant valence is as well.", "gemini": "Bộ não phải quyết định có nên phản ứng với các kích thích được phát hiện hay không và phản ứng như thế nào dựa trên đầu vào cảm giác phức tạp. Ruồi giấm Drosophila melanogaster đánh giá nguồn thức ăn dựa trên các tín hiệu khứu giác. Ở đây, chúng tôi đã thực hiện sàng lọc hành vi bằng cách sử dụng ruồi giấm và thiết lập giá trị bẩm sinh của 110 chất tạo mùi. Phân tích của chúng tôi về các kiểu kích hoạt của nơron do các chất tạo mùi hấp dẫn và gây khó chịu gợi ý rằng mặc dù bản chất của các chất tạo mùi được mã hóa bởi tập hợp các thụ thể được kích hoạt, nhưng biểu hiện chính của giá trị mùi được hình thành ở mức đầu ra của thùy ăng ten. Sự tập hợp theo địa hình bên trong thùy ăng ten của các nơron đầu ra đặc hiệu về giá trị giống với một miền tương ứng trong bóng khứu giác của chuột. Cấu trúc giải phẫu cơ bản của mạch khứu giác giữa côn trùng và động vật có xương sống được biết là tương tự nhau; nghiên cứu của chúng tôi cho thấy rằng biểu hiện của giá trị mùi cũng vậy."} +{"idx": 1990016, "en": "A pilot-scale integrated membrane-aerated biofilm reactor (MABR) system, consisted of hydrolysis/acidification pretreatment, MABR process and activated carbon adsorption post-processing, was designed to treat the high-loading mixed pharmaceutical wastewater. A study of MABR process was conducted to investigate the effect of aeration condition, circulation flow rate and water quality on performance over 260 days. The performances of these processes were evaluated by the removal efficiency of COD, BOD(5), turbidity, NH(4)(+)-N and TN. MABR process could effectively remove above 90% of COD and 98% of ammonia. The capacities per unit volume of MABR could reach up to 1311 gCOD/m(3)d and 48.2 gNH(4)(+)-N/m(3)d with single membrane aeration, and the oxygen utilization rate could be as high as 45%. After post-processing, the effluent of integrated treatment MABR system kept stable with COD below 200 mg/L and NH(4)(+)-N below 3 mg/L.", "gemini": "Một hệ thống lò phản ứng màng sinh học sục khí tích hợp quy mô thí điểm (MABR), bao gồm tiền xử lý thủy phân/axit hóa, quy trình MABR và xử lý sau bằng hấp phụ than hoạt tính, đã được thiết kế để xử lý nước thải dược phẩm hỗn hợp có tải lượng cao. Một nghiên cứu về quy trình MABR đã được tiến hành để điều tra ảnh hưởng của điều kiện sục khí, lưu lượng tuần hoàn và chất lượng nước đến hiệu suất trong hơn 260 ngày. Hiệu suất của các quy trình này được đánh giá bằng hiệu suất loại bỏ COD, BOD(5), độ đục, NH(4)(+)-N và TN. Quy trình MABR có thể loại bỏ hiệu quả trên 90% COD và 98% amoniac. Công suất trên một đơn vị thể tích của MABR có thể đạt tới 1311 gCOD/m(3)d và 48,2 gNH(4)(+)-N/m(3)d với sục khí màng đơn và tỷ lệ sử dụng oxy có thể cao tới 45%. Sau khi xử lý sau, nước thải của hệ thống MABR xử lý tích hợp vẫn ổn định với COD dưới 200 mg/L và NH(4)(+)-N dưới 3 mg/L."} +{"idx": 1990017, "en": "The connection between the Hedgehog (HH) pathway and cholesterol has been recognized since the early days that shaped our current understanding of this unique pathway. Cholesterol and related lipids are intricately linked to HH signaling: from the role of cholesterol in HH biosynthesis to the modulation of HH signal reception and transduction by other sterols, passing by the phylogenetic relationships among many components of the HH pathway that resemble or contain lipid-binding domains. Here I review the connections between HH signaling, cholesterol and its derivatives and analyze the potential implications for HH-dependent cancers.", "gemini": "Mối liên hệ giữa con đường Hedgehog (HH) và cholesterol đã được công nhận từ những ngày đầu hình thành nên sự hiểu biết hiện tại của chúng ta về con đường độc đáo này. Cholesterol và các lipid liên quan có liên quan chặt chẽ đến tín hiệu HH: từ vai trò của cholesterol trong quá trình sinh tổng hợp HH đến việc điều chế quá trình tiếp nhận và truyền tín hiệu HH bởi các sterol khác, đi qua các mối quan hệ phát sinh loài giữa nhiều thành phần của con đường HH giống hoặc chứa các miền liên kết lipid. Ở đây, tôi xem xét các kết nối giữa tín hiệu HH, cholesterol và các dẫn xuất của nó và phân tích các tác động tiềm tàng đối với các loại ung thư phụ thuộc HH."} +{"idx": 1990018, "en": "Oxysterols/oxysterol receptors have been shown to modulate several immune cell subsets, such as macrophages, T-cells and B-cells, neutrophils and dendritic cells (DCs). They participate in the control of several pathologic processes, that is, infectious diseases, atherosclerosis and autoimmunity. Moreover, some oxysterols have also been shown to favor tumor progression by dampening the antitumor immune response. The cellular responses generated by oxysterols depend on the engagement of Liver X Receptor (LXR) α and/or β isoforms, which induce activation of target genes or trans-repression of pro-inflammatory gene transcription. Recently, some reports have described a different mechanism of action of oxysterols, mediated by the engagement of G-Protein Coupled Receptors. Here, we summarize LXR-dependent and LXR-independent responses of oxysterols on immune cells with possible effects on tumor development.", "gemini": "Oxysterol/ thụ thể oxysterol đã được chứng minh là điều hòa một số phân nhóm tế bào miễn dịch, chẳng hạn như đại thực bào, tế bào T và tế bào B, bạch cầu trung tính và tế bào trình diện kháng nguyên (DC). Chúng tham gia kiểm soát một số quá trình bệnh lý, tức là bệnh truyền nhiễm, xơ vữa động mạch và tự miễn dịch. Hơn nữa, một số oxysterol cũng đã được chứng minh là ủng hộ sự tiến triển của khối u bằng cách làm giảm phản ứng miễn dịch chống khối u. Các phản ứng của tế bào do oxysterol tạo ra phụ thuộc vào sự gắn kết của các đồng dạng thụ thể X gan (LXR) α và/hoặc β, gây ra sự hoạt hóa của các gen đích hoặc sự ức chế chuyển gen của gen gây viêm. Gần đây, một số báo cáo đã mô tả một cơ chế hoạt động khác của oxysterol, được trung gian bởi sự gắn kết của các thụ thể liên hợp protein G. Ở đây, chúng tôi tóm tắt các phản ứng phụ thuộc LXR và không phụ thuộc LXR của oxysterol trên các tế bào miễn dịch với các tác động có thể xảy ra đối với sự phát triển khối u."} +{"idx": 1990019, "en": "In order to meet the challenges of our current medical need to address infections caused by highly resistant pathogens, we propose the use of superiority trial designs. The proposed trials may or may not be statistically powered. All require extensive preclinical justification. The designs could use either historical or active controls. For historically controlled trials we propose two approaches for defining the control response rate to therapy; (1) the use of pharmacometrics from modern trials and (2) the use of a concurrent observational study. Designs for active controlled trials could be (1) standard of care+test vs. standard of care alone or (2) standard of care vs. test article. The second approach requires extensive justification to show that the test article will be of sufficient efficacy to allow ethical use as a single agent.", "gemini": "Để đáp ứng những thách thức của nhu cầu y tế hiện tại của chúng ta nhằm giải quyết các bệnh nhiễm trùng do các tác nhân gây bệnh có khả năng kháng thuốc cao, chúng tôi đề xuất sử dụng các thiết kế thử nghiệm vượt trội. Các thử nghiệm được đề xuất có thể hoặc không có đủ năng lực thống kê. Tất cả đều yêu cầu chứng minh tiền lâm sàng mở rộng. Các thiết kế có thể sử dụng các đối chứng trong quá khứ hoặc hiện tại. Đối với các thử nghiệm có đối chứng trong quá khứ, chúng tôi đề xuất hai cách tiếp cận để xác định tỷ lệ đáp ứng của đối chứng với liệu pháp; (1) sử dụng dược động học từ các thử nghiệm hiện đại và (2) sử dụng một nghiên cứu quan sát đồng thời. Thiết kế cho các thử nghiệm có đối chứng chủ động có thể là (1) tiêu chuẩn chăm sóc + thử nghiệm so với chỉ tiêu chuẩn chăm sóc hoặc (2) tiêu chuẩn chăm sóc so với bài kiểm tra. Cách tiếp cận thứ hai đòi hỏi phải chứng minh rộng rãi để chỉ ra rằng bài kiểm tra sẽ có hiệu quả đủ để cho phép sử dụng một cách hợp lý như một tác nhân duy nhất."} +{"idx": 1990020, "en": "The 3 study objectives were to compare the activity levels of older people who had received a restorative home care service with those of people who had received \"usual\" home care, explore the predictors of physical activity in these 2 groups, and determine whether either group met the minimum recommended activity levels for their age group. A questionnaire was posted to 1,490 clients who had been referred for a home care service between 2006 and 2009. Older people who had received a restorative care service were more active than those who had received usual care (p = .049), but service group did not predict activity levels when other variables were adjusted for in a multiple regression. Younger individuals who were in better physical condition, with good mobility and no diagnosis of depression, were more likely to be active. Investigation of alternatives to the current exercise component of the restorative program is needed.", "gemini": "3 mục tiêu nghiên cứu là so sánh mức độ hoạt động của những người cao tuổi đã nhận được dịch vụ chăm sóc tại nhà phục hồi chức năng với những người đã nhận được dịch vụ chăm sóc tại nhà \"thông thường\", khám phá các yếu tố dự báo hoạt động thể chất ở 2 nhóm này và xác định xem có nhóm nào đáp ứng được mức hoạt động tối thiểu được khuyến nghị cho nhóm tuổi của họ hay không. Một bảng câu hỏi đã được gửi đến 1.490 khách hàng đã được giới thiệu đến dịch vụ chăm sóc tại nhà trong khoảng thời gian từ năm 2006 đến năm 2009. Những người cao tuổi đã nhận được dịch vụ chăm sóc phục hồi chức năng hoạt động nhiều hơn những người đã nhận được dịch vụ chăm sóc thông thường (p = 0,049), nhưng nhóm dịch vụ không dự đoán mức độ hoạt động khi các biến khác được điều chỉnh trong hồi quy đa. Những người trẻ tuổi có thể trạng tốt hơn, khả năng vận động tốt và không được chẩn đoán mắc chứng trầm cảm có khả năng hoạt động nhiều hơn. Cần nghiên cứu các phương án thay thế cho thành phần tập thể dục hiện tại của chương trình phục hồi chức năng."} +{"idx": 1990021, "en": "BACKGROUND Although donepezil, an acetylcholinesterase inhibitor, has been proved to be effective in ameliorating cognitive impairment in Parkinson's disease with dementia (PDD), the responsiveness of patients to donepezil therapy varies. [5-(11)C-methoxy]donepezil, the radiolabeled form of donepezil, is a ligand for positron emission tomography (PET), which can be exploited for the quantitative analysis of donepezil binding to acetylcholinesterase and for cholinergic imaging. OBJECTIVES To investigate the deficits of the cholinergic system in the brain in PDD and its association with response to donepezil therapy. METHODS Twelve patients with PDD and 13 normal control subjects underwent [5-(11)C-methoxy]donepezil-PET imaging. For patients with PDD, daily administration of donepezil was started after [5-(11)C-methoxy]donepezil-PET imaging and continued for 3 months. RESULTS In the PDD group, the mean total distribution volume of the cerebral cortices was 22.7% lower than that of the normal control group. The mean total distribution volume of the patients with PDD was significantly correlated with improvement of visuoperceptual function after 3 months of donepezil therapy. CONCLUSION The results suggest that donepezil therapy is more effective in patients with less decrease in acetylcholinesterase, a binding site of donepezil, at least in the specific cognitive domain.", "gemini": "BỐI CẢNH Mặc dù donepezil, một chất ức chế acetylcholinesterase, đã được chứng minh là có hiệu quả trong việc cải thiện suy giảm nhận thức trong bệnh Parkinson có sa sút trí tuệ (PDD), nhưng khả năng đáp ứng của bệnh nhân với liệu pháp donepezil lại khác nhau. [5-(11)C-methoxy]donepezil, dạng đánh dấu phóng xạ của donepezil, là một phối tử cho chụp cắt lớp phát xạ positron (PET), có thể được khai thác để phân tích định lượng liên kết donepezil với acetylcholinesterase và để chụp ảnh cholinergic. MỤC TIÊU Để nghiên cứu sự thiếu hụt của hệ cholinergic trong não ở PDD và mối liên quan của nó với đáp ứng với liệu pháp donepezil. PHƯƠNG PHÁP Mười hai bệnh nhân mắc PDD và 13 đối tượng đối chứng bình thường đã được chụp ảnh [5-(11)C-methoxy]donepezil-PET. Đối với những bệnh nhân mắc PDD, việc dùng donepezil hàng ngày được bắt đầu sau khi chụp ảnh [5-(11)C-methoxy]donepezil-PET và tiếp tục trong 3 tháng. KẾT QUẢ Ở nhóm PDD, thể tích phân bố trung bình của vỏ não thấp hơn 22,7% so với nhóm đối chứng bình thường. Thể tích phân bố trung bình của bệnh nhân PDD có mối tương quan đáng kể với sự cải thiện chức năng thị giác sau 3 tháng điều trị donepezil. KẾT LUẬN Kết quả cho thấy liệu pháp donepezil có hiệu quả hơn ở những bệnh nhân giảm ít hơn acetylcholinesterase, một vị trí liên kết của donepezil, ít nhất là trong phạm vi nhận thức cụ thể."} +{"idx": 1990022, "en": "Fever is a major component of the host's defense against infection. Inadequate febrile response can predispose an individual to the deleterious effects of infection. Neonatal exposure to infectious agents such as bacterial lipopolysaccharide (LPS) permanently dampens the adult febrile response. Whether prenatal immune challenge alters febrile response during adulthood is still not known. In the present study, LPS (100 µg/kg, i.p.) or pyrogen-free saline was administered to pregnant rats on either gestation day (GD) 12, 15 or 19 and the febrile response of their respective adult offspring was monitored. During adulthood (>70 days old), the rats born to LPS-injected dams on GD15 displayed a significantly attenuated febrile response to LPS (50 µg/kg, i.p.) compared to their control counterparts born to dams given saline on GD15. Immune challenge during either early (GD12) or late (GD19) pregnancy did not have a significant impact on fever in the adult offspring. Immune challenge on GD15, but not on GD12 or 19, heightened the plasma corticosterone response to a subsequent LPS injection to the adult offspring but did not have a significant effect on their basal plasma corticosterone levels. Finally, LPS-induced COX-2 in the fever-controlling regions of the hypothalamus was significantly reduced in the adult rats born to dams given LPS on GD15 compared to their counterparts born to dams given saline on GD15. Such COX-2 reduction was not observed in the adult offspring born to dams given LPS on either GD12 or 19. Taken together, these data suggest that a single immune challenge during a critical window of pregnancy alters the neuroimmune response in adult offspring.", "gemini": "Sốt là một thành phần chính trong hệ thống phòng vệ của vật chủ chống lại nhiễm trùng. Phản ứng sốt không đầy đủ có thể khiến một cá thể dễ bị ảnh hưởng có hại của nhiễm trùng. Tiếp xúc với các tác nhân truyền nhiễm như lipopolysaccharide (LPS) của vi khuẩn ở thời kỳ sơ sinh sẽ làm giảm vĩnh viễn phản ứng sốt ở người lớn. Liệu thử thách miễn dịch trước sinh có làm thay đổi phản ứng sốt ở tuổi trưởng thành hay không vẫn chưa được biết. Trong nghiên cứu hiện tại, LPS (100 µg/kg, i.p.) hoặc dung dịch muối không gây sốt đã được dùng cho những con chuột mang thai vào ngày mang thai (GD) 12, 15 hoặc 19 và phản ứng sốt của con trưởng thành tương ứng của chúng được theo dõi. Trong thời kỳ trưởng thành (>70 ngày tuổi), những con chuột được sinh ra từ những con mẹ được tiêm LPS vào GD15 biểu hiện phản ứng sốt giảm đáng kể đối với LPS (50 µg/kg, i.p.) so với những con đối chứng được sinh ra từ những con mẹ được tiêm dung dịch muối vào GD15. Thử thách miễn dịch trong thời kỳ đầu mang thai (GD12) hoặc cuối (GD19) không có tác động đáng kể đến cơn sốt ở con trưởng thành. Thử thách miễn dịch vào GD15, nhưng không phải vào GD12 hoặc 19, làm tăng phản ứng corticosterone trong huyết tương đối với một mũi tiêm LPS tiếp theo ở con trưởng thành nhưng không có tác động đáng kể đến nồng độ corticosterone cơ bản trong huyết tương của chúng. Cuối cùng, COX-2 do LPS gây ra ở các vùng kiểm soát sốt của vùng dưới đồi giảm đáng kể ở những con chuột trưởng thành được sinh ra từ những con mẹ được tiêm LPS vào GD15 so với những con được sinh ra từ những con mẹ được tiêm dung dịch muối vào GD15. Sự giảm COX-2 như vậy không được quan sát thấy ở những con trưởng thành được sinh ra từ những con mẹ được tiêm LPS vào GD12 hoặc 19. Kết hợp lại, những dữ liệu này cho thấy rằng một thử thách miễn dịch duy nhất trong một thời kỳ quan trọng của thai kỳ làm thay đổi phản ứng miễn dịch thần kinh ở con trưởng thành."} +{"idx": 1990023, "en": "Syntactic operations in language and music are well established and known to be linked in cognitive and neuroanatomical terms. What remains a matter of debate is whether the notion of syntax also applies to human actions and how those may be linked to syntax in language and music. The present electroencephalography (EEG) study explored syntactic processes during the observation, motor programming, and execution of musical actions. Therefore, expert pianists watched and imitated silent videos of a hand playing 5-chord sequences in which the last chord was syntactically congruent or incongruent with the preceding harmonic context. 2-chord sequences that diluted the syntactic predictability of the last chord (by reducing the harmonic context) served as a control condition. We assumed that behavioural and event-related potential (ERP) effects (i.e., differences between congruent and incongruent trials) that were significantly stronger in the 5-chord compared to the 2-chord sequences are related to syntactic processing. According to this criterion, the present results show an influence of syntactic context on ERPs related to (i) action observation and (ii) the motor programming for action imitation, as well as (iii) participants' execution times and accuracy. In particular, the occurrence of electrophysiological indices of action inhibition and reprogramming when an incongruent chord had to be imitated implies that the pianist's motor system anticipated (and revoked) the congruent chord during action observation. Notably, this well-known anticipatory potential of the motor system seems to be strongly based upon the observer's music-syntactic knowledge, thus suggesting the \"embodied\" processing of musical syntax. The combined behavioural and electrophysiological data show that the notion of musical syntax not only applies to the auditory modality but transfers--in trained musicians--to a \"grammar of musical action\".", "gemini": "Các hoạt động cú pháp trong ngôn ngữ và âm nhạc đã được thiết lập tốt và được biết là có liên quan về mặt nhận thức và giải phẫu thần kinh. Vấn đề vẫn còn gây tranh cãi là liệu khái niệm cú pháp cũng áp dụng cho hành động của con người hay không và những hành động đó có thể liên quan đến cú pháp trong ngôn ngữ và âm nhạc như thế nào. Nghiên cứu điện não đồ (EEG) hiện tại đã khám phá các quá trình cú pháp trong quá trình quan sát, lập trình vận động và thực hiện các hành động âm nhạc. Do đó, các nghệ sĩ piano chuyên nghiệp đã xem và bắt chước các video im lặng về một bàn tay chơi các chuỗi hợp âm 5 nốt trong đó hợp âm cuối cùng phù hợp hoặc không phù hợp với ngữ cảnh hòa âm trước đó. Các chuỗi hợp âm 2 nốt làm giảm khả năng dự đoán cú pháp của hợp âm cuối cùng (bằng cách giảm ngữ cảnh hòa âm) đóng vai trò là điều kiện kiểm soát. Chúng tôi cho rằng các hiệu ứng tiềm năng liên quan đến hành vi và sự kiện (ERP) (tức là sự khác biệt giữa các thử nghiệm phù hợp và không phù hợp) mạnh hơn đáng kể trong chuỗi 5 hợp âm so với chuỗi 2 hợp âm có liên quan đến xử lý cú pháp. Theo tiêu chuẩn này, các kết quả hiện tại cho thấy ảnh hưởng của ngữ cảnh cú pháp trên ERP liên quan đến (i) quan sát hành động và (ii) lập trình vận động để bắt chước hành động, cũng như (iii) thời gian thực hiện và độ chính xác của người tham gia. Đặc biệt, sự xuất hiện của các chỉ số điện sinh lý về ức chế hành động và lập trình lại khi một hợp âm không phù hợp phải được bắt chước ngụ ý rằng hệ thống vận động của nghệ sĩ piano đã dự đoán (và thu hồi) hợp âm phù hợp trong quá trình quan sát hành động. Đáng chú ý, tiềm năng dự đoán được biết đến này của hệ thống vận động dường như dựa chủ yếu vào kiến thức cú pháp âm nhạc của người quan sát, do đó gợi ý quá trình xử lý \"thể hiện\" cú pháp âm nhạc. Dữ liệu hành vi và điện sinh lý kết hợp cho thấy rằng khái niệm cú pháp âm nhạc không chỉ áp dụng cho phương thức thính giác mà còn chuyển sang \"ngữ pháp hành động âm nhạc\" ở những nhạc sĩ được đào tạo."} +{"idx": 1990024, "en": "OBJECTIVE To compare the characteristics of acute mastoiditis in children in different age groups in order to identify risk groups and risk factors for acute mastoiditis. METHODS Records for all children aged 0-16 years treated for acute mastoiditis during 1993-2007 at 33 Ear, Nose and Throat departments in Sweden were reviewed retrospectively according to defined criteria for acute mastoiditis. RESULTS A total of 678 cases fulfilled the inclusion criteria. Acute mastoiditis was most common in children younger than two years of age and this group was characterized by less prior history of other diseases and ear diseases, fewer visits to health care centers and less antibiotic treatment before admission, shorter duration of symptoms before admission, hospitalization for fewer days and lower frequency of complications and mastoidectomies. These children also showed a higher incidence of clinical findings, increased inflammatory markers such as fever and heightened counts of C-reactive protein and white blood cells compared with older children. They also tested positive for significantly more samples of Streptococcus pneumoniae while the older children more often exhibited growth of Streptococcus pyogenes or Pseudomonas aeruginosa or no microbial growth. CONCLUSIONS The characteristics of pediatric acute mastoiditis differed significantly between age groups. Acute mastoiditis was most common in children younger than two years of age. They showed more rapid progress of symptoms and more distinct signs of acute mastoiditis. This is probably the reason why parents rapidly seek medical care for small children and hospital treatment thus starts earlier in the youngest children, which may in turn explain the excellent outcome. This study showed that younger children have neither more severe acute mastoiditis nor more complications than older ones. The differences between age groups suggest that there are distinctions in the pathophysiology behind the onset and course of acute mastoiditis in younger and older children.", "gemini": "MỤC TIÊU So sánh các đặc điểm của viêm xương chũm cấp ở trẻ em ở các nhóm tuổi khác nhau để xác định các nhóm nguy cơ và các yếu tố nguy cơ gây viêm xương chũm cấp. PHƯƠNG PHÁP Hồ sơ bệnh án của tất cả trẻ em từ 0-16 tuổi được điều trị viêm xương chũm cấp trong giai đoạn 1993-2007 tại 33 khoa Tai, Mũi và Họng ở Thụy Điển đã được xem xét hồi cứu theo các tiêu chuẩn xác định đối với viêm xương chũm cấp. KẾT QUẢ Tổng cộng có 678 trường hợp đáp ứng các tiêu chuẩn tuyển chọn. Viêm xương chũm cấp phổ biến nhất ở trẻ em dưới hai tuổi và nhóm này được đặc trưng bởi tiền sử mắc các bệnh khác và các bệnh về tai ít hơn, ít đến các trung tâm chăm sóc sức khỏe hơn và ít được điều trị bằng kháng sinh trước khi nhập viện, thời gian biểu hiện triệu chứng trước khi nhập viện ngắn hơn, thời gian nằm viện ít ngày hơn và tần suất các biến chứng và phẫu thuật cắt xương chũm thấp hơn. Những trẻ này cũng biểu hiện tỷ lệ các triệu chứng lâm sàng cao hơn, các dấu hiệu viêm tăng chẳng hạn như sốt và số lượng protein C phản ứng và bạch cầu tăng so với trẻ lớn hơn. Chúng cũng có kết quả xét nghiệm dương tính với nhiều mẫu Streptococcus pneumoniae hơn trong khi trẻ lớn hơn thường biểu hiện sự phát triển của Streptococcus pyogenes hoặc Pseudomonas aeruginosa hoặc không có sự phát triển của vi khuẩn. KẾT LUẬN Các đặc điểm của viêm xương chũm cấp ở trẻ em khác nhau đáng kể giữa các nhóm tuổi. Viêm xương chũm cấp phổ biến nhất ở trẻ em dưới hai tuổi. Chúng biểu hiện tiến triển nhanh hơn các triệu chứng và các dấu hiệu rõ ràng hơn của viêm xương chũm cấp. Đây có thể là lý do tại sao cha mẹ nhanh chóng tìm kiếm sự chăm sóc y tế cho trẻ nhỏ và do đó điều trị tại bệnh viện bắt đầu sớm hơn ở trẻ nhỏ nhất, từ đó có thể giải thích kết quả tuyệt vời. Nghiên cứu này cho thấy trẻ nhỏ không bị viêm xương chũm cấp nghiêm trọng hơn hoặc có nhiều biến chứng hơn trẻ lớn. Sự khác biệt giữa các nhóm tuổi cho thấy có sự khác biệt về sinh lý bệnh đằng sau sự khởi phát và diễn biến của viêm xương chũm cấp ở trẻ nhỏ và trẻ lớn."} +{"idx": 1990025, "en": "OBJECTIVE The aim of this study is to evaluate endoscopic assisted sublabial and buccolabial incision approach as treatment option for Juvenile nasopharyngeal angiofibroma (JNA) with extensive infratemporal fossa (ITF) extension. METHODS Seven patients diagnosed as JNA with extensive ITF extension and underwent surgery at our department between 2006 and 2010 were retrospectively reviewed. All patients underwent diagnostic arteriography followed by embolization preoperatively and intratumor injection with N-butyl cyanoacrylate (NBCA). Endoscopic assisted sublabial and buccolabial incision approach was used to remove the tumors. RESULTS Complete resections of the tumors were achieved in all patients. Mean blood loss was 700 ml. The follow-up period ranged from 9 to 20 months, with a mean of 14.3 months. One patient had a recurrent tumor six months after operation. CONCLUSIONS Endoscopic assisted sublabial and buccolabial incision is an optional approach to remove JNA with extensive ITF extension.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu này là đánh giá phương pháp tiếp cận rạch dưới môi và má hỗ trợ nội soi như một lựa chọn điều trị cho u sợi mạch máu vòm hầu ở trẻ em (JNA) với phần mở rộng hố thái dương (ITF) rộng. PHƯƠNG PHÁP Bảy bệnh nhân được chẩn đoán là JNA với phần mở rộng ITF rộng và đã phẫu thuật tại khoa của chúng tôi từ năm 2006 đến năm 2010 đã được xem xét hồi cứu. Tất cả bệnh nhân đều được chụp động mạch chẩn đoán sau đó là nút mạch trước phẫu thuật và tiêm vào khối u bằng N-butyl cyanoacrylate (NBCA). Phương pháp tiếp cận rạch dưới môi và má hỗ trợ nội soi đã được sử dụng để cắt bỏ khối u. KẾT QUẢ Tất cả bệnh nhân đều cắt bỏ hoàn toàn khối u. Lượng máu mất trung bình là 700 ml. Thời gian theo dõi dao động từ 9 đến 20 tháng, trung bình là 14,3 tháng. Một bệnh nhân bị tái phát khối u sáu tháng sau khi phẫu thuật. KẾT LUẬN Rạch dưới môi và má hỗ trợ nội soi là một phương pháp tiếp cận tùy chọn để cắt bỏ JNA với phần mở rộng ITF rộng."} +{"idx": 1990026, "en": "OBJECTIVE This study evaluated whether a change in the content of matrix microvesicles might occur at the preatherosclerotic stage. METHODS Applying quantitative electron microscopic and immunohistochemical analyses, two areas of grossly normal segments of the thoracic aorta were compared: atherosclerosis-prone (AP) areas, situated at the dorsal aspect of the aorta along the rows of intercostal branch origins, and atherosclerosis-resistant (AR) areas, situated at the corresponding sites of the ventral aspect of the aorta. RESULTS The electron microscopic analysis showed that there were 1.4 times more microvesicles in AP areas than AR areas (p = 0.019). It was found that matrix microvesicles originated as a result of blebbing and shedding of surface membranes of smooth muscle cells. A quantitative analysis of the expression of ADP-ribosylation factor 6 (ARF6), which is known to be involved in membrane trafficking and microvesicle formation, showed that ARF6 expression was 1.3 times higher in AP areas than that in AR areas (p = 0.006). There was a positive correlation between the content of matrix microparticles and the expression of ARF6 by intimal smooth muscle cells (r = 0.61; p < 0.0001). CONCLUSION The present study supports the concept that alterations of the arterial intima occur at the predisease stage.", "gemini": "MỤC TIÊU Nghiên cứu này đánh giá xem sự thay đổi về thành phần của các túi nhỏ trong ma trận có thể xảy ra ở giai đoạn tiền xơ vữa động mạch hay không. PHƯƠNG PHÁP Áp dụng các phân tích định lượng về kính hiển vi điện tử và miễn dịch mô hóa học, hai vùng của các đoạn bình thường của động mạch chủ ngực được so sánh: các vùng dễ bị xơ vữa động mạch (AP), nằm ở mặt lưng của động mạch chủ dọc theo các hàng nguồn nhánh liên sườn và vùng kháng xơ vữa động mạch (AR), nằm ở các vị trí tương ứng của mặt bụng của động mạch chủ. KẾT QUẢ Phân tích kính hiển vi điện tử cho thấy có nhiều túi nhỏ hơn 1,4 lần ở vùng AP so với vùng AR (p = 0,019). Người ta thấy rằng các túi nhỏ trong ma trận bắt nguồn từ sự phồng rộp và bong tróc màng bề mặt của các tế bào cơ trơn. Một phân tích định lượng về sự biểu hiện của yếu tố ribosyl hóa ADP 6 (ARF6), được biết là có liên quan đến quá trình vận chuyển màng và hình thành túi nhỏ, cho thấy biểu hiện ARF6 cao hơn 1,3 lần ở vùng AP so với vùng AR (p = 0,006). Có mối tương quan tích cực giữa hàm lượng các hạt nhỏ trong ma trận và biểu hiện ARF6 của các tế bào cơ trơn nội mạc (r = 0,61; p < 0,0001). KẾT LUẬN Nghiên cứu hiện tại ủng hộ khái niệm rằng các biến đổi của lớp nội mạc động mạch xảy ra ở giai đoạn tiền bệnh."} +{"idx": 1990027, "en": "This off-line study aims to assess the performance of five classifiers commonly used in the brain-computer interface (BCI) community, when applied to a gaze-independent P300-based BCI. In particular, we compared the results of four linear classifiers and one nonlinear: Fisher's linear discriminant analysis (LDA), stepwise linear discriminant analysis (SWLDA), Bayesian linear discriminant analysis (BLDA), linear support vector machine (LSVM) and Gaussian supported vector machine (GSVM). Moreover, different values for the decimation of the training dataset were tested. The results were evaluated both in terms of accuracy and written symbol rate with the data of 19 healthy subjects. No significant differences among the considered classifiers were found. The optimal decimation factor spanned a range from 3 to 24 (12 to 94 ms long bins). Nevertheless, performance on individually optimized classification parameters is not significantly different from a classification with general parameters (i.e. using an LDA classifier, about 48 ms long bins).", "gemini": "Nghiên cứu ngoại tuyến này nhằm đánh giá hiệu suất của năm bộ phân loại thường được sử dụng trong cộng đồng giao diện não-máy tính (BCI), khi áp dụng cho BCI dựa trên P300 độc lập với ánh nhìn. Cụ thể, chúng tôi đã so sánh kết quả của bốn bộ phân loại tuyến tính và một bộ phi tuyến tính: phân tích phân biệt tuyến tính của Fisher (LDA), phân tích phân biệt tuyến tính từng bước (SWLDA), phân tích phân biệt tuyến tính Bayesian (BLDA), máy vectơ hỗ trợ tuyến tính (LSVM) và máy vectơ hỗ trợ Gauss (GSVM). Hơn nữa, các giá trị khác nhau để giảm tập dữ liệu huấn luyện đã được kiểm tra. Kết quả được đánh giá cả về độ chính xác và tốc độ ký hiệu được viết bằng dữ liệu của 19 đối tượng khỏe mạnh. Không tìm thấy sự khác biệt đáng kể giữa các bộ phân loại được xem xét. Hệ số giảm tối ưu trải dài trong phạm vi từ 3 đến 24 (thùng dài 12 đến 94 ms). Tuy nhiên, hiệu suất trên các thông số phân loại được tối ưu hóa riêng lẻ không khác biệt đáng kể so với phân loại với các thông số chung (tức là sử dụng bộ phân loại LDA, thùng dài khoảng 48 ms)."} +{"idx": 1990028, "en": "AIM To determine the incidence of umbilical cord prolapse, the characteristics of the population, and to evaluate its management and the neonatal prognosis. MATERIAL AND METHODS Ninety-three cases of cord prolapse that occurred between January 1986 and December 2009 at our level III labour ward were studied retrospectively. RESULTS The incidence of cord prolapse was 0.18%. It occurred in 66.7% of cases in multiparous patients, in 19.4% of cases in twin pregnancies, and in 41.9% of cases in breech presentations. In 34.4% of cases, the gestational age was less than 37 weeks. Birth occurred vaginally in 33.3% of cases with a delivery time interval significantly less than for caesarean sections (P<0.001). At complete cervical dilation, more than three quarter of patients delivered vaginally. Vaginal birth was significantly more frequent in case of breech (P=0.009) and second twin (P=0.03). Parity did not influence birth route. Neonates with a birth weight less than 2500 g (30.1%) had significantly more frequently an Apgar score less than 7 at 5 min (P=0.02), a higher rate of transfer to intensive care (P<0.001) and a longer hospital stay (P=0.002). We report six neonatal deaths (6.5%). Neonatal status was not influenced by the time interval for delivery. CONCLUSION Umbilical cord prolapse is still nowadays a serious complication of pregnancy, responsible for a significant rate of neonatal mortality. The aim in case of cord prolapse is to obtain fetal delivery the quickest way possible so as to improve the neonatal outcome. In some particular obstetrical situations such as breech presentations and second twin deliveries, birth occurs faster if performed vaginally as shown by our case study.", "gemini": "MỤC ĐÍCH Xác định tỷ lệ sa dây rốn, đặc điểm của quần thể và đánh giá cách xử trí cũng như tiên lượng ở trẻ sơ sinh. CHẤT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu hồi cứu 93 trường hợp sa dây rốn xảy ra từ tháng 1 năm 1986 đến tháng 12 năm 2009 tại khoa sản cấp III của chúng tôi. KẾT QUẢ Tỷ lệ sa dây rốn là 0,18%. Tình trạng này xảy ra ở 66,7% trường hợp ở những bệnh nhân đa thai, 19,4% trường hợp ở những thai đôi và 41,9% trường hợp ở ngôi ngược. Ở 34,4% trường hợp, tuổi thai dưới 37 tuần. Sinh ngả âm đạo ở 33,3% trường hợp với khoảng thời gian chuyển dạ ngắn hơn đáng kể so với sinh mổ (P < 0,001). Khi cổ tử cung mở hết, hơn ba phần tư bệnh nhân sinh ngả âm đạo. Sinh ngả âm đạo thường xuyên hơn đáng kể trong trường hợp ngôi ngược (P = 0,009) và song thai thứ hai (P = 0,03). Số lần sinh không ảnh hưởng đến đường sinh. Trẻ sơ sinh có cân nặng khi sinh dưới 2500 g (30,1%) thường xuyên có điểm Apgar dưới 7 ở phút thứ 5 (P = 0,02), tỷ lệ chuyển đến khoa chăm sóc đặc biệt cao hơn (P < 0,001) và thời gian nằm viện lâu hơn (P = 0,002). Chúng tôi báo cáo có sáu trẻ sơ sinh tử vong (6,5%). Tình trạng của trẻ sơ sinh không bị ảnh hưởng bởi khoảng thời gian chuyển dạ. KẾT LUẬN Sa dây rốn cho đến nay vẫn là một biến chứng nghiêm trọng của thai kỳ, gây ra tỷ lệ tử vong ở trẻ sơ sinh đáng kể. Mục đích trong trường hợp sa dây rốn là thực hiện chuyển dạ nhanh nhất có thể để cải thiện kết cục ở trẻ sơ sinh. Trong một số tình huống sản khoa đặc biệt như ngôi ngược và chuyển dạ song thai thứ hai, sinh ngả âm đạo diễn ra nhanh hơn như nghiên cứu trường hợp của chúng tôi đã chỉ ra."} +{"idx": 1990029, "en": "Self-assembled nanostructures of α,ω-dihexylsexithiophene (DH6T) formed by spreading DH6T solutions onto freshly cleaved mica surface were studied by atomic force microscopy. The effects of solvent and concentration on the nanostructures of DH6T molecules were studied. Flat, well-ordered, and platelet-like domains were observed on mica surfaces after treatment with various polar solvent solutions of DH6T. These domains form a uniform film with height of 2.4 ± 0.2 nm, which is consistent with a 45° tilt in the molecular conformation of DH6T on mica surfaces. The formation mechanism of these multilayers is discussed in detail.", "gemini": "Các cấu trúc nano tự lắp ráp của α,ω-dihexylsexithiophene (DH6T) được hình thành bằng cách trải các dung dịch DH6T lên bề mặt mica mới tách đã được nghiên cứu bằng kính hiển vi lực nguyên tử. Các tác động của dung môi và nồng độ lên các cấu trúc nano của phân tử DH6T đã được nghiên cứu. Các miền phẳng, có trật tự tốt và giống như tiểu cầu đã được quan sát thấy trên bề mặt mica sau khi xử lý bằng nhiều dung dịch dung môi phân cực của DH6T. Các miền này tạo thành một màng đồng nhất có chiều cao 2,4 ± 0,2 nm, phù hợp với độ nghiêng 45° trong cấu hình phân tử của DH6T trên bề mặt mica. Cơ chế hình thành của các lớp đa lớp này được thảo luận chi tiết."} +{"idx": 1990030, "en": "Preeclampsia is a pregnancy-specific syndrome mainly characterized by hypertensive disorder and proteinuria after gestational weeks 20. So far the etiology of preeclampsia remains unclear. We previously reported that preeclamptic placentas exhibited decreased mRNA expression and hypermethylation in promoter region of the paternally imprinted H19 gene compared with normal placentas. H19 has recently been identified to encode the precursor of miR-675, indicating a possible novel functional pathway of the imprinting gene. The aim of the present study was to identify the roles of H19 gene via miR-675 pathway in human trophoblast cells, and to figure out the involvement of this pathway in pathogenesis of preeclampsia. Knockdown of H19 gene or inhibition of miR-675 exhibited similar proliferation-promoting effect in human trophoblastic JEG-3 cells. Target gene prediction in combination with luciferase assay revealed that miR-675 could directly downregulate Nodal Modulator 1 (NOMO1) protein expression by binding to 3'-UTR sequence of NOMO1. Overexpression of NOMO1 in JEG-3 cells could rescue miR-675-surppressed cell proliferation and phosphorylation of Smad2, while Nodal had additive effect with miR-675 in suppression cell proliferation and activation of Smad2. In early-onset preeclamptic placentas, expression levels of H19 gene and miR-675 were appreciably lower, while NOMO1 protein level was higher than those in normal placentas. Taken together, our data suggested that H19 gene could inhibit human trophoblast cell proliferation via encoding miR-675 that targeted NOMO1, and aberrantly lowered expression of H19 in placenta may participate in the excessive proliferation of trophoblast cells observed in early-onset severe preeclampsia by downregulating miR-675 which targets NOMO1 and interferes with Nodal signaling.", "gemini": "Tiền sản giật là một hội chứng đặc hiệu của thai kỳ, chủ yếu đặc trưng bởi rối loạn tăng huyết áp và protein niệu sau tuần thứ 20 của thai kỳ. Cho đến nay, nguyên nhân gây ra tiền sản giật vẫn chưa rõ ràng. Trước đây, chúng tôi đã báo cáo rằng nhau thai tiền sản giật biểu hiện giảm biểu hiện mRNA và tăng metyl hóa ở vùng khởi động của gen H19 được đóng dấu cha mẹ so với nhau thai bình thường. Gần đây, H19 đã được xác định là mã hóa tiền chất của miR-675, chỉ ra một con đường chức năng mới có thể có của gen đóng dấu. Mục đích của nghiên cứu hiện tại là xác định vai trò của gen H19 thông qua con đường miR-675 trong các tế bào nuôi dưỡng bào thai của người và tìm ra sự liên quan của con đường này trong sinh bệnh học của tiền sản giật. Việc hạ gục gen H19 hoặc ức chế miR-675 đều biểu hiện tác dụng thúc đẩy tăng sinh tương tự trong các tế bào JEG-3 nuôi dưỡng bào thai của người. Dự đoán gen đích kết hợp với xét nghiệm luciferase cho thấy miR-675 có thể trực tiếp điều chỉnh giảm biểu hiện protein Nodal Modulator 1 (NOMO1) bằng cách liên kết với trình tự 3'-UTR của NOMO1. Sự biểu hiện quá mức của NOMO1 trong các tế bào JEG-3 có thể cứu vãn sự tăng sinh tế bào bị miR-675 ức chế và quá trình phosphoryl hóa Smad2, trong khi Nodal có tác dụng cộng gộp với miR-675 trong việc ức chế tăng sinh tế bào và hoạt hóa Smad2. Ở nhau thai tiền sản giật khởi phát sớm, mức biểu hiện của gen H19 và miR-675 thấp hơn đáng kể, trong khi mức protein NOMO1 cao hơn so với nhau thai bình thường. Kết hợp lại, dữ liệu của chúng tôi cho thấy gen H19 có thể ức chế sự tăng sinh tế bào nuôi dưỡng bào thai của người thông qua việc mã hóa miR-675 nhắm vào NOMO1, và sự biểu hiện thấp bất thường của H19 trong nhau thai có thể tham gia vào sự tăng sinh quá mức của các tế bào nuôi dưỡng bào thai được quan sát thấy trong tiền sản giật nặng khởi phát sớm bằng cách điều chỉnh giảm miR-675 nhắm vào NOMO1 và can thiệp vào tín hiệu Nodal."} +{"idx": 1990031, "en": "Guided by miRNAs, RNAi plays an important role in virus-host interactions by fine-tuning gene expression. Many viral and cellular miRNAs are involved in virus infection, though no comprehensive general model for miRNAs derived from invertebrate DNA viruses exists for their function in eukaryotic systems, despite extensive research on miRNAs. To address this issue, the miRNAs from shrimp white spot syndrome virus (WSSV), a DNA virus with a 305 kb double-stranded circular DNA genome, were characterized. Based on WSSV miRNA microarray and northern blot analyses, WSSV was shown to possess the capacity to encode 40 distinct viral miRNAs, a miRNA content roughly 360 times greater than that of humans. These findings suggested that the high content of viral miRNAs might greatly contribute to viral variability in response selective pressures in the host environment. Transcription analysis revealed that 80% of WSSV miRNAs were expressed during early stages of viral infection, indicating their importance in initial infective processes. Additionally, biogenesis of viral miRNAs was demonstrated to be dependent on host Drosha and Dicer 1, mediated by Ago 1, and viral miRNAs, including WSSV-miR211 and WSSV-miR212, were required for successful WSSV infection. During WSSV infection, numerous viral genes were likely targeted by WSSV miRNAs. The current study presented the first comprehensive view of viral miRNAs encoded by an invertebrate DNA virus, providing insight into the molecular events of virus-host interactions.", "gemini": "Được hướng dẫn bởi các miRNA, RNAi đóng một vai trò quan trọng trong tương tác giữa vi-rút và vật chủ bằng cách điều chỉnh chính xác biểu hiện gen. Nhiều miRNA của vi-rút và tế bào tham gia vào quá trình nhiễm vi-rút, mặc dù không có mô hình chung toàn diện nào cho các miRNA có nguồn gốc từ vi-rút DNA không xương sống tồn tại đối với chức năng của chúng trong các hệ thống nhân chuẩn, mặc dù đã có nhiều nghiên cứu sâu rộng về miRNA. Để giải quyết vấn đề này, các miRNA từ vi-rút hội chứng đốm trắng ở tôm (WSSV), một loại vi-rút DNA có bộ gen DNA vòng kép 305 kb, đã được đặc trưng. Dựa trên các phân tích microarray miRNA của WSSV và chuẩn độ miền bắc, WSSV đã được chứng minh là có khả năng mã hóa 40 miRNA của vi-rút riêng biệt, hàm lượng miRNA cao hơn khoảng 360 lần so với ở người. Những phát hiện này cho thấy rằng hàm lượng miRNA của vi-rút cao có thể đóng góp rất lớn vào khả năng biến đổi của vi-rút để đáp ứng áp lực chọn lọc trong môi trường vật chủ. Phân tích phiên mã cho thấy 80% miRNA của WSSV được biểu hiện trong giai đoạn đầu của quá trình nhiễm vi-rút, cho thấy tầm quan trọng của chúng trong các quá trình nhiễm trùng ban đầu. Ngoài ra, quá trình sinh tổng hợp miRNA của vi-rút đã được chứng minh là phụ thuộc vào Drosha và Dicer 1 của vật chủ, được trung gian bởi Ago 1, và các miRNA của vi-rút, bao gồm WSSV-miR211 và WSSV-miR212, là cần thiết để nhiễm WSSV thành công. Trong quá trình nhiễm WSSV, nhiều gen của vi-rút có khả năng bị nhắm mục tiêu bởi các miRNA của WSSV. Nghiên cứu hiện tại trình bày cái nhìn toàn diện đầu tiên về các miRNA của vi-rút được mã hóa bởi một loại vi-rút DNA không xương sống, cung cấp cái nhìn sâu sắc về các sự kiện phân tử của tương tác giữa vi-rút và vật chủ."} +{"idx": 1990032, "en": "Correct codon-anticodon pairing promotes translational fidelity, with these interactions greatly facilitated by modified nucleosides found in tRNA. We hypothesized that wobble uridine modifications catalyzed by tRNA methyltransferase 9 (Trm9) are essential for translational fidelity. In support, we have used phenotypic, reporter and protein-based assays to demonstrate increased translational infidelity in trm9Δ Saccharomyces cerevisiae cells. Codon reengineering studies suggest that Trm9-catalyzed tRNA modifications promote fidelity during the translation of specific genes, those rich in arginine and glutamic acid codons from mixed boxes. Using quantitative tRNA modification analysis, we determined that trm9Δ cells are only deficient in 2 of 23 tRNA modifications, with those 2, 5-methoxycarbonylmethyluridine (mcm ( 5) U) and 5-methoxycarbonylmethyl-2-thiouridine (mcm ( 5) s ( 2) U), classified as key determinants of translational fidelity. We also show that in the absence of mcm ( 5) U and mcm ( 5) s ( 2) U, the resulting translational infidelity promotes protein errors and activation of unfolded protein and heat shock responses. These data support a model in which Trm9-catalyzed tRNA modifications promote fidelity during the translation of specific transcripts, with decreased wobble base modification leading to translational infidelity, protein errors and activation of protein stress response pathways.", "gemini": "Sự ghép cặp codon-anticodon chính xác thúc đẩy độ trung thực của quá trình dịch mã, với các tương tác này được tạo điều kiện thuận lợi rất nhiều bởi các nucleoside được sửa đổi có trong tRNA. Chúng tôi giả thuyết rằng các sửa đổi uridine dao động do tRNA methyltransferase 9 (Trm9) xúc tác là rất cần thiết cho độ trung thực của quá trình dịch mã. Để hỗ trợ, chúng tôi đã sử dụng các xét nghiệm dựa trên kiểu hình, báo cáo và protein để chứng minh sự không trung thực của quá trình dịch mã tăng lên trong các tế bào trm9Δ Saccharomyces cerevisiae. Các nghiên cứu tái tạo mã codon cho thấy các sửa đổi tRNA do Trm9 xúc tác thúc đẩy độ trung thực trong quá trình dịch mã của các gen cụ thể, những gen giàu codon arginine và axit glutamic từ các hộp hỗn hợp. Sử dụng phân tích sửa đổi tRNA định lượng, chúng tôi xác định rằng các tế bào trm9Δ chỉ thiếu 2 trong số 23 sửa đổi tRNA, với 2 sửa đổi đó là 5-methoxycarbonylmethyluridine (mcm (5) U) và 5-methoxycarbonylmethyl-2-thiouridine (mcm (5) s (2) U), được phân loại là các yếu tố quyết định chính của độ trung thực của quá trình dịch mã. Chúng tôi cũng chỉ ra rằng khi không có mcm (5) U và mcm (5) s (2) U, sự không trung thực của quá trình dịch mã kết quả sẽ thúc đẩy các lỗi protein và kích hoạt các phản ứng protein chưa gấp và sốc nhiệt. Các dữ liệu này hỗ trợ một mô hình trong đó các sửa đổi tRNA do Trm9 xúc tác thúc đẩy độ trung thực trong quá trình dịch mã của các bản phiên mã cụ thể, với việc giảm sửa đổi bazơ dao động dẫn đến sự không trung thực của quá trình dịch mã, lỗi protein và kích hoạt các con đường phản ứng căng thẳng protein."} +{"idx": 1990033, "en": "The inclusion of a potent TLR4 immune potentiator to a recombinant antigen vaccine formulation enhances both the magnitude and the breadth of the engendered immune response. One such immune potentiator (TLR4 agonist E6020) was evaluated with recombinant Men B antigens delivered in MF59 sub-micron adjuvant emulsion. The ability of this formulation to enhance serum antibody and bactercidal titers was investigated. The co-delivery of E6020 within MF59 enhanced both the serum and bactericidal titers for Men B antigens and for Men B antigens combined with Men ACWY-CRM conjugate vaccine. The delivery of TLR4 agonist within MF59 emulsion oil droplets leads to a more potent response in comparison to the TLR4 when admixed with MF59 emulsion.", "gemini": "Việc đưa một chất tăng cường miễn dịch TLR4 mạnh vào công thức vắc-xin kháng nguyên tái tổ hợp làm tăng cả cường độ và phạm vi của phản ứng miễn dịch được tạo ra. Một chất tăng cường miễn dịch như vậy (chất chủ vận TLR4 E6020) đã được đánh giá với các kháng nguyên Men B tái tổ hợp được đưa vào nhũ tương tá dược dưới micron MF59. Khả năng của công thức này trong việc tăng cường kháng thể huyết thanh và nồng độ diệt khuẩn đã được nghiên cứu. Việc đồng cung cấp E6020 trong các giọt dầu nhũ tương MF59 làm tăng cả nồng độ huyết thanh và diệt khuẩn đối với các kháng nguyên Men B và đối với các kháng nguyên Men B kết hợp với vắc-xin liên hợp Men ACWY-CRM. Việc đưa chất chủ vận TLR4 vào trong các giọt dầu nhũ tương MF59 dẫn đến phản ứng mạnh hơn so với TLR4 khi trộn với nhũ tương MF59."} +{"idx": 1990034, "en": "Sipuleucel-T (Provenge) (Sip-T) is first -in class as a therapeutic autologous vaccine approved for the treatment of men with asymptomatic or minimally symptomatic castrate-resistant metastatic prostate cancer. This product is the culmination of decades of basic immunological and prostate cancer investigations and 13 y of clinical trial investigations. Sip-T represents a paradigm shift in cancer therapeutics and represents the first approved autologous therapeutic cancer vaccine, which has demonstrated a survival benefit. The potential benefit of this product is the excellent risk to benefit ratio, which will allow for the combination of this approach with other more toxic therapies. The favorable risk to benefit will also afford the opportunity for trials investigating this product earlier in the disease state and in combination with local therapies. The ability to target more localized or lower volume disease will maximize the therapeutic benefit over a longer period of time. The novelty of the platform of this approach could be used to treat any cancer with a tumor-specific cell surface target. The main product of Sip-T is the re-infusion of a patient's antigen presenting cells from leukapheresis after ex-vivo exposure to a chimeric protein of human GM-CSF and PAP. In metastatic CRPC patients three infusions of these activated cells over a month lead to statistically significant 4.1 mo increase in median survival and a 22.5% reduction in risk of death. The main side effect from this re-infusion of activated immune cells is a \"flu-like\" syndrome that includes chills, fatigue, fevers, back pain, nausea, joints aches and headaches in decreasing order of frequency. Immune monitoring during the clinical trials also demonstrated a specific cellular and antibody immune response, suggesting the proposed mechanism of adoptive immunotherapy to PAP was behind this survival benefit. This product also serves as a proof of principle for targeted immunotherapy for others cancers with defined cell surface markers. In summary, the approval of Sip-T based on a survival benefit and very tolerable safety profile will 1) enhance our ability to care for men with advanced prostate cancer, 2) allow for further investigations of this approach in combination with others therapies with different mechanisms of action and non-overlapping toxicities, and 3) allow further investigations earlier in the course of the disease.", "gemini": "Sipuleucel-T (Provenge) (Sip-T) là sản phẩm đầu tiên trong nhóm vắc-xin tự thân trị liệu được chấp thuận để điều trị cho nam giới mắc ung thư tuyến tiền liệt di căn kháng thiến không có triệu chứng hoặc có triệu chứng tối thiểu. Sản phẩm này là sự đúc kết của nhiều thập kỷ nghiên cứu về miễn dịch học cơ bản và ung thư tuyến tiền liệt và 13 năm nghiên cứu thử nghiệm lâm sàng. Sip-T đại diện cho sự thay đổi mô hình trong điều trị ung thư và là vắc-xin ung thư tự thân trị liệu đầu tiên được chấp thuận, đã chứng minh được lợi ích về khả năng kéo dài sự sống. Lợi ích tiềm tàng của sản phẩm này là tỷ lệ rủi ro trên lợi ích tuyệt vời, cho phép kết hợp phương pháp tiếp cận này với các liệu pháp độc hại hơn khác. Tỷ lệ rủi ro trên lợi ích thuận lợi cũng sẽ mang lại cơ hội cho các thử nghiệm nghiên cứu sản phẩm này sớm hơn trong tình trạng bệnh và kết hợp với các liệu pháp tại chỗ. Khả năng nhắm mục tiêu vào bệnh cục bộ hơn hoặc khối lượng bệnh ít hơn sẽ tối đa hóa lợi ích điều trị trong thời gian dài hơn. Tính mới lạ của nền tảng của phương pháp tiếp cận này có thể được sử dụng để điều trị bất kỳ loại ung thư nào có mục tiêu bề mặt tế bào đặc hiệu của khối u. Sản phẩm chính của Sip-T là truyền lại các tế bào trình diện kháng nguyên của bệnh nhân từ quá trình lọc bạch cầu sau khi tiếp xúc ngoài cơ thể với protein ghép nối của GM-CSF và PAP của người. Ở những bệnh nhân CRPC di căn, ba lần truyền những tế bào hoạt hóa này trong một tháng dẫn đến sự gia tăng đáng kể về mặt thống kê là 4,1 tháng về thời gian sống trung bình và giảm 22,5% nguy cơ tử vong. Tác dụng phụ chính từ việc truyền lại các tế bào miễn dịch hoạt hóa này là hội chứng \"giống cúm\" bao gồm ớn lạnh, mệt mỏi, sốt, đau lưng, buồn nôn, đau khớp và nhức đầu theo thứ tự tần suất giảm dần. Việc theo dõi miễn dịch trong các thử nghiệm lâm sàng cũng chứng minh được phản ứng miễn dịch tế bào và kháng thể đặc hiệu, cho thấy cơ chế đề xuất của liệu pháp miễn dịch thụ động đối với PAP là nguyên nhân dẫn đến lợi ích về khả năng kéo dài sự sống này. Sản phẩm này cũng đóng vai trò như một bằng chứng về nguyên tắc cho liệu pháp miễn dịch có mục tiêu đối với các bệnh ung thư khác có các dấu hiệu bề mặt tế bào được xác định. Tóm lại, việc chấp thuận Sip-T dựa trên lợi ích về khả năng kéo dài sự sống và hồ sơ an toàn rất dung nạp sẽ 1) tăng cường khả năng chăm sóc của chúng tôi đối với những người đàn ông mắc bệnh ung thư tuyến tiền liệt tiến triển, 2) cho phép nghiên cứu sâu hơn về phương pháp tiếp cận này kết hợp với các liệu pháp khác có cơ chế tác động khác nhau và độc tính không chồng chéo, và 3) cho phép nghiên cứu sâu hơn sớm hơn trong quá trình của bệnh."} +{"idx": 1990035, "en": "In non-mandatory vaccination policies, individual choice can be a major driver of vaccine uptake. Choice thereby influences whether public health targets can be achieved. Individual vaccinating decisions can be influenced by perceptions of vaccine risks or infection risks. There is also the potential for non-vaccinators to strategically 'free-ride' on herd immunity provided by vaccinators. This strategic interaction between individuals generates a social dilemma--a conflict between self-interest and what is best for the group as a whole. Game theory and related mathematical approaches that couple mechanistic models of vaccinating decisions with mechanistic models of disease spread can capture this social dilemma and address relevant questions. The past decade has seen significant growth in the theoretical literature developing and analyzing such models. Here, we argue that using these models to address specific public health challenges will require more work that integrates information from empirical studies into the development and validation of such models, as well as more collaboration between mathematical modelers, psychologists, economists and public health experts.", "gemini": "Trong các chính sách tiêm chủng không bắt buộc, lựa chọn của cá nhân có thể là động lực chính thúc đẩy việc tiêm vắc-xin. Do đó, sự lựa chọn ảnh hưởng đến việc có thể đạt được các mục tiêu y tế công cộng hay không. Quyết định tiêm chủng của cá nhân có thể bị ảnh hưởng bởi nhận thức về rủi ro của vắc-xin hoặc rủi ro nhiễm trùng. Ngoài ra, những người không tiêm vắc-xin có khả năng \"ăn theo\" miễn dịch cộng đồng do những người tiêm vắc-xin cung cấp một cách chiến lược. Tương tác chiến lược này giữa các cá nhân tạo ra một tình thế tiến thoái lưỡng nan về mặt xã hội - một cuộc xung đột giữa lợi ích cá nhân và lợi ích tốt nhất cho toàn thể nhóm. Lý thuyết trò chơi và các phương pháp toán học liên quan kết hợp các mô hình cơ học về quyết định tiêm vắc-xin với các mô hình cơ học về sự lây lan của bệnh có thể nắm bắt được tình thế tiến thoái lưỡng nan về mặt xã hội này và giải quyết các câu hỏi có liên quan. Trong thập kỷ qua, đã có sự phát triển đáng kể trong các tài liệu lý thuyết về việc phát triển và phân tích các mô hình như vậy. Tại đây, chúng tôi lập luận rằng việc sử dụng các mô hình này để giải quyết các thách thức cụ thể về sức khỏe cộng đồng sẽ đòi hỏi nhiều công việc hơn nữa tích hợp thông tin từ các nghiên cứu thực nghiệm vào quá trình phát triển và xác thực các mô hình như vậy, cũng như tăng cường hợp tác giữa các nhà mô hình toán học, nhà tâm lý học, nhà kinh tế học và các chuyên gia y tế công cộng."} +{"idx": 1990036, "en": "Dengue, endemic in Puerto Rico, is a major public health problem. Vaccines are thought the best means to prevent dengue because vector control alone has been largely ineffective. We implemented qualitative studies in 2006 and 2010 to determine the acceptability of conducting placebo-controlled dengue vaccine efficacy trials in Puerto Rican children. Key informant interviews and focus groups with parents and children were conducted in municipalities with high dengue incidence. We used structured open-ended questions to determine motivators and attitudes regarding vaccine trial participation. Knowledge about dengue risk and prevention, and knowledge, attitudes, and beliefs regarding vaccines and vaccine trials were assessed. Using grounded theory, we conducted content analysis and established categories and sub-categories of participant responses. All participants were knowledgeable about dengue prevention and perceived children as most affected age groups. Participants were aware of vaccines benefits and they thought a vaccine could prevent dengue. However, most would not allow their children to participate in a placebo-controlled vaccine trial. Barriers included lack of trust in new vaccines and vaccine trial procedures; fear of developing dengue or side effects from the vaccine and lack of information about candidate dengue vaccines. Participants thought information, including results of previous trials might overcome barriers to participation. Motivators for participation were altruism, protection from dengue, free medical attention, and compensation for transportation and participation. Parents would consider children participation if accurate vaccine trial information is provided.", "gemini": "Sốt xuất huyết, bệnh đặc hữu ở Puerto Rico, là một vấn đề sức khỏe cộng đồng lớn. Vắc-xin được coi là phương tiện tốt nhất để phòng ngừa sốt xuất huyết vì chỉ kiểm soát véc-tơ thì phần lớn là không hiệu quả. Chúng tôi đã triển khai các nghiên cứu định tính vào năm 2006 và 2010 để xác định mức độ chấp nhận tiến hành các thử nghiệm hiệu quả vắc-xin sốt xuất huyết có đối chứng giả dược ở trẻ em Puerto Rico. Các cuộc phỏng vấn người cung cấp thông tin chính và các nhóm tập trung với cha mẹ và trẻ em đã được tiến hành tại các thành phố có tỷ lệ mắc sốt xuất huyết cao. Chúng tôi đã sử dụng các câu hỏi mở có cấu trúc để xác định động lực và thái độ liên quan đến việc tham gia thử nghiệm vắc-xin. Kiến thức về nguy cơ và phòng ngừa sốt xuất huyết, cũng như kiến thức, thái độ và niềm tin liên quan đến vắc-xin và thử nghiệm vắc-xin đã được đánh giá. Sử dụng lý thuyết cơ bản, chúng tôi đã tiến hành phân tích nội dung và thiết lập các danh mục và tiểu danh mục về phản hồi của người tham gia. Tất cả những người tham gia đều có hiểu biết về phòng ngừa sốt xuất huyết và coi trẻ em là nhóm tuổi bị ảnh hưởng nhiều nhất. Những người tham gia nhận thức được lợi ích của vắc-xin và họ nghĩ rằng vắc-xin có thể ngăn ngừa sốt xuất huyết. Tuy nhiên, hầu hết sẽ không cho phép con mình tham gia thử nghiệm vắc-xin có đối chứng giả dược. Các rào cản bao gồm thiếu tin tưởng vào vắc-xin mới và các thủ tục thử nghiệm vắc-xin; sợ mắc sốt xuất huyết hoặc tác dụng phụ của vắc-xin và thiếu thông tin về các loại vắc-xin sốt xuất huyết ứng viên. Những người tham gia cho rằng thông tin, bao gồm cả kết quả của các thử nghiệm trước đó có thể vượt qua các rào cản tham gia. Động lực tham gia là lòng vị tha, bảo vệ khỏi sốt xuất huyết, được chăm sóc y tế miễn phí và được bồi thường cho việc đi lại và tham gia. Cha mẹ sẽ xem xét việc cho con tham gia nếu được cung cấp thông tin chính xác về thử nghiệm vắc-xin."} +{"idx": 1990037, "en": "Neisseria meningitidis serogroup B (MnB) is a significant cause of invasive meningococcal disease, but no broadly protective vaccine is yet approved. We assessed the safety and immunogenicity of a bivalent MnB vaccine composed of lipidated subfamily A and B variants of recombinant LP2086 (rLP2086, also known as factor H binding protein, fHBP). Forty-eight adults, ages 18-40 y, were randomized to receive 60, 120 or 200 μg of the bivalent rLP2086 vaccine or control at 0, 2 and 6 mo. Immunogenicity was assessed by rLP2086-specific immunoglobulin G (IgG) geometric mean titers for subfamily A and B proteins. Safety was determined by laboratory assessments of blood and urine and by reporting of solicited and unsolicited adverse events (AEs). The bivalent rLP2086 vaccine elicited high IgG titers following the second and third vaccination at all dose levels. In each of the four study arms, 11 of the 12 participating subjects reported ≥ 1 AE, and no serious AEs were reported. Local and systemic reactions were mainly mild to moderate. Laboratory abnormalities (including increased sodium, decreased neutrophils, and proteinuria) were not associated with clinical events and were not considered to be related to the study vaccine. Vaccinations were generally well-tolerated. Strong IgG antibody responses and the absence of clinically significant laboratory abnormalities support further development of the bivalent rLP2086 vaccine (www.clinicaltrials.gov; identifier: NCT00879814).", "gemini": "Neisseria meningitidis nhóm huyết thanh B (MnB) là một nguyên nhân đáng kể gây ra bệnh não mô cầu xâm lấn, nhưng hiện vẫn chưa có vắc-xin bảo vệ rộng rãi nào được chấp thuận. Chúng tôi đã đánh giá tính an toàn và khả năng sinh miễn dịch của vắc-xin MnB hai giá trị gồm các biến thể lipidated của phân họ A và B của LP2086 tái tổ hợp (rLP2086, còn được gọi là protein liên kết yếu tố H, fHBP). Bốn mươi tám người lớn, độ tuổi từ 18-40, được phân ngẫu nhiên để nhận 60, 120 hoặc 200 μg vắc-xin rLP2086 hai giá trị hoặc đối chứng vào thời điểm 0, 2 và 6 tháng. Khả năng sinh miễn dịch được đánh giá bằng giá trị trung bình hình học của immunoglobulin G (IgG) đặc hiệu rLP2086 đối với protein phân họ A và B. Tính an toàn được xác định bằng các đánh giá trong phòng thí nghiệm về máu và nước tiểu và bằng cách báo cáo các tác dụng phụ (AE) được yêu cầu và không được yêu cầu. Vắc-xin rLP2086 hai giá trị tạo ra giá trị IgG cao sau lần tiêm thứ hai và thứ ba ở tất cả các mức liều. Trong mỗi bốn nhánh nghiên cứu, 11 trong số 12 đối tượng tham gia đã báo cáo ≥ 1 AE và không có AE nghiêm trọng nào được báo cáo. Các phản ứng tại chỗ và toàn thân chủ yếu ở mức nhẹ đến trung bình. Các bất thường về xét nghiệm (bao gồm tăng natri, giảm bạch cầu trung tính và protein niệu) không liên quan đến các sự kiện lâm sàng và không được coi là liên quan đến vắc-xin nghiên cứu. Việc tiêm chủng nói chung được dung nạp tốt. Phản ứng kháng thể IgG mạnh và không có bất thường về xét nghiệm có ý nghĩa lâm sàng hỗ trợ việc phát triển thêm vắc-xin rLP2086 hai giá trị (www.clinicaltrials.gov; mã số: NCT00879814)."} +{"idx": 1990038, "en": "This study evaluated safety, tolerability and immunogenicity of intradermal (ID) trivalent inactivated split influenza vaccine, with a lower antigen content (9 mcg HA per strain) than the conventional intramuscular one (15 mcg), in HIV-1-infected adults younger than 60 years. A total of 54 HIV-1-positive participants were enrolled and randomly assigned to receive a single dose of either ID-administered low-antigen-content split inactivated vaccine or intramuscularly-administered (IM) standard-dose inactivated split vaccine. Subjects were provided with a diary to monitor any local and/or systemic reactions to the vaccine for 7 days following vaccination. Serum samples were collected before, 28 days and 90 days after immunization. The plasma HIV-RNA and CD4+ T-lymphocyte count were checked at day 0 and day 90. Serum hemagglutination-inhibition (HI) activity for the three influenza strains included in the vaccine composition was measured to assess the antibody response at one month and 3 months after vaccination. Both vaccines showed optimal safety and tolerability profiles. All the three Committee for Medicinal Products for Human Use immunogenicity criteria for vaccine approval in adults younger than 60 were met by both vaccines against A(H1N1) and A(H3N2) viruses. Both vaccines met mean-fold-increase and seroprotection criteria but failed seroconversion criteria against B virus. No difference in terms of post-vaccination geometric mean titers, mean fold increase, seroprotection and seroconversion rates were found comparing ID and IM vaccines. In conclusion, the recently available low-antigen-content ID vaccine is safe, well-tolerated and as immunogenic as IM standard-dose influenza vaccine.", "gemini": "Nghiên cứu này đánh giá tính an toàn, khả năng dung nạp và sinh miễn dịch của vắc-xin cúm chia tách bất hoạt ba giá trị trong da (ID), có hàm lượng kháng nguyên thấp hơn (9 mcg HA trên mỗi chủng) so với vắc-xin tiêm bắp thông thường (15 mcg), ở người lớn nhiễm HIV-1 dưới 60 tuổi. Tổng cộng 54 người tham gia dương tính với HIV-1 đã được tuyển dụng và được chỉ định ngẫu nhiên để nhận một liều vắc-xin phân tách bất hoạt có hàm lượng kháng nguyên thấp do ID tiêm hoặc vắc-xin phân tách bất hoạt liều chuẩn do tiêm bắp (IM). Những người tham gia được cung cấp một cuốn nhật ký để theo dõi bất kỳ phản ứng tại chỗ và/hoặc toàn thân nào đối với vắc-xin trong 7 ngày sau khi tiêm chủng. Các mẫu huyết thanh được thu thập trước, 28 ngày và 90 ngày sau khi tiêm chủng. Lượng HIV-RNA trong huyết tương và số lượng tế bào lympho T CD4+ được kiểm tra vào ngày 0 và ngày 90. Hoạt động ức chế ngưng kết hồng cầu (HI) trong huyết thanh đối với ba chủng cúm có trong thành phần vắc-xin được đo để đánh giá đáp ứng kháng thể sau một tháng và 3 tháng sau khi tiêm chủng. Cả hai loại vắc-xin đều cho thấy hồ sơ an toàn và khả năng dung nạp tối ưu. Cả hai loại vắc-xin đều đáp ứng cả ba tiêu chí về khả năng sinh miễn dịch của Ủy ban sản phẩm thuốc dùng cho người để chấp thuận vắc-xin ở người lớn dưới 60 tuổi đối với vi-rút A (H1N1) và A (H3N2). Cả hai loại vắc-xin đều đáp ứng các tiêu chí tăng gấp bội trung bình và bảo vệ huyết thanh nhưng không đáp ứng các tiêu chí chuyển đổi huyết thanh đối với vi-rút B. Không có sự khác biệt về giá trị trung bình hình học sau tiêm chủng, tăng gấp bội trung bình, tỷ lệ bảo vệ huyết thanh và chuyển đổi huyết thanh khi so sánh vắc-xin ID và IM. Tóm lại, vắc-xin ID có hàm lượng kháng nguyên thấp mới có sẵn gần đây là an toàn, được dung nạp tốt và có khả năng sinh miễn dịch như vắc-xin cúm liều chuẩn IM."} +{"idx": 1990039, "en": "OBJECTIVE In addition to increased risks for aneurysm-related death, previous studies have determined that all-cause mortality in abdominal aortic aneurysm (AAA) patients is excessive and equivalent to that associated with coronary heart disease. These studies largely preceded the current era of coronary heart disease risk factor management, however, and no recent study has examined contemporary mortality associated with early AAA disease (aneurysm diameter between 3 and 5 cm). As part of an ongoing natural history study of AAA, we report the mortality risk associated with presence of early disease. METHODS Participants were recruited from three distinct health care systems in Northern California between 2006 and 2011. Aneurysm diameter, demographic information, comorbidities, medication history, and plasma for biomarker analysis were collected at study entry. Survival status was determined at follow-up. Data were analyzed with t-tests or χ(2) tests where appropriate. Freedom from death was calculated via Cox proportional hazards modeling; the relevance of individual predictors on mortality was determined by log-rank test. RESULTS The study enrolled 634 AAA patients; age 76.4 ± 8.0 years, aortic diameter 3.86 ± 0.7 cm. Participants were mostly male (88.8%), not current smokers (81.6%), and taking statins (76.7%). Mean follow-up was 2.1 ± 1.0 years. Estimated 1- and 3-year survival was 98.2% and 90.9%, respectively. Factors independently associated with mortality included larger aneurysm size (hazard ratio, 2.12; 95% confidence interval, 1.26-3.57 for diameter >4.0 cm) and diabetes (hazard ratio, 2.24; 95% confidence interval, 1.12-4.47). After adjusting for patient-level factors, health care system independently predicted mortality. CONCLUSIONS Contemporary all-cause mortality for patients with early AAA disease is lower than that previously reported. Further research is warranted to determine important factors that contribute to improved survival in early AAA disease.", "gemini": "MỤC TIÊU Ngoài nguy cơ tử vong do phình động mạch tăng lên, các nghiên cứu trước đây đã xác định rằng tỷ lệ tử vong do mọi nguyên nhân ở những bệnh nhân phình động mạch chủ bụng (AAA) là quá mức và tương đương với tỷ lệ liên quan đến bệnh tim mạch vành. Tuy nhiên, phần lớn các nghiên cứu này đã diễn ra trước thời đại hiện tại của việc quản lý các yếu tố nguy cơ bệnh tim mạch vành và không có nghiên cứu gần đây nào xem xét tỷ lệ tử vong đương thời liên quan đến bệnh AAA giai đoạn đầu (đường kính phình động mạch từ 3 đến 5 cm). Là một phần của nghiên cứu tiền sử tự nhiên đang diễn ra của AAA, chúng tôi báo cáo nguy cơ tử vong liên quan đến sự hiện diện của bệnh giai đoạn đầu. PHƯƠNG PHÁP Những người tham gia được tuyển dụng từ ba hệ thống chăm sóc sức khỏe riêng biệt ở Bắc California từ năm 2006 đến 2011. Đường kính phình động mạch, thông tin nhân khẩu học, bệnh đi kèm, tiền sử dùng thuốc và huyết tương để phân tích sinh học được thu thập khi vào nghiên cứu. Tình trạng sống sót được xác định khi theo dõi. Dữ liệu được phân tích bằng các bài kiểm tra t hoặc χ(2) khi thích hợp. Tỷ lệ không tử vong được tính thông qua mô hình rủi ro tỷ lệ Cox; sự liên quan của từng yếu tố dự báo đối với tỷ lệ tử vong được xác định bằng kiểm tra log-rank. KẾT QUẢ Nghiên cứu đã tuyển dụng 634 bệnh nhân AAA; tuổi 76,4 ± 8,0, đường kính động mạch chủ 3,86 ± 0,7 cm. Những người tham gia chủ yếu là nam giới (88,8%), không hút thuốc hiện tại (81,6%) và dùng statin (76,7%). Thời gian theo dõi trung bình là 2,1 ± 1,0 năm. Tỷ lệ sống sót ước tính sau 1 và 3 năm lần lượt là 98,2% và 90,9%. Các yếu tố có liên quan độc lập với tỷ lệ tử vong bao gồm kích thước phình động mạch lớn hơn (tỷ lệ nguy cơ, 2,12; khoảng tin cậy 95%, 1,26-3,57 đối với đường kính> 4,0 cm) và bệnh tiểu đường (tỷ lệ nguy cơ, 2,24; khoảng tin cậy 95%, 1,12-4,47). Sau khi điều chỉnh theo các yếu tố cấp độ bệnh nhân, hệ thống chăm sóc sức khỏe dự đoán độc lập tỷ lệ tử vong. KẾT LUẬN Tỷ lệ tử vong do mọi nguyên nhân đương thời đối với những bệnh nhân mắc bệnh AAA giai đoạn đầu thấp hơn so với báo cáo trước đây. Cần tiến hành nghiên cứu thêm để xác định các yếu tố quan trọng góp phần cải thiện tỷ lệ sống sót ở bệnh AAA giai đoạn đầu."} +{"idx": 1990040, "en": "A 66-year-old man with an abdominal aortic aneurysm previously repaired with an endovascular stent graft presented to our facility with worsening midabdominal and back pain. Previous postoperative surveillance computed tomography scans were unremarkable, showing excellent stent-wall apposition and a shrinking aneurysm sac; however, imaging done on his arrival identified a contained rupture at the level of the celiac artery containing a perforating suprarenal stent. We repaired this rupture with a surgeon-modified fenestrated stent graft. To our knowledge, this is the first reported case of penetration of the native aorta by a suprarenal stent in the absence of infection or trauma.", "gemini": "Một người đàn ông 66 tuổi bị phình động mạch chủ bụng trước đây đã được sửa chữa bằng ghép stent nội mạch đã đến cơ sở của chúng tôi với tình trạng đau lưng và bụng giữa ngày càng trầm trọng. Chụp cắt lớp vi tính giám sát sau phẫu thuật trước đó không có gì đáng chú ý, cho thấy sự tiếp giáp thành stent tuyệt vời và túi phình động mạch co lại; tuy nhiên, hình ảnh chụp khi ông đến đã xác định vị trí vỡ ở mức động mạch celiac chứa một stent xuyên thận thượng. Chúng tôi đã sửa chữa vết vỡ này bằng ghép stent có lỗ do bác sĩ phẫu thuật sửa đổi. Theo chúng tôi biết, đây là trường hợp đầu tiên được báo cáo về tình trạng xuyên động mạch chủ tự nhiên bởi một stent thận thượng khi không có nhiễm trùng hoặc chấn thương."} +{"idx": 1990041, "en": "A series 6-aryl-pyrrolo[2,3-d]pyrimidine-4-amines (43 compounds), some of which are epidermal growth factor tyrosine kinase inhibitors, were tested for their protozoal toxicity using an environmental Tetrahymena strain as model organism. The protozoacidal activity of the analogues was found to be highly dependent on a 4-hydroxyl group at the 6-aryl ring, and a chiral 1-phenylethanamine substituent in position 4. Further, the potency was affected by the aromatic substitution pattern of the phenylethanamine: the unsubstituted, the meta-fluoro and the para-bromo substituted derivatives had the lowest minimum protozoacidal concentrations (8-16 μg/mL). Surprisingly, both enantiomers were found to have high potency suggesting that this compound class could have several modes of action. No correlation was found between the compounds protozoacidal activity and the in vitro epidermal growth factor receptor tyrosine kinase inhibitory potency. This suggests that the observed antimicrobial effects are related to other targets. Testing towards a panel of kinases indicated several alternative modes of action.", "gemini": "Một loạt 6-aryl-pyrrolo[2,3-d]pyrimidine-4-amine (43 hợp chất), một số trong số đó là chất ức chế tyrosine kinase của yếu tố tăng trưởng biểu bì, đã được thử nghiệm độc tính với động vật nguyên sinh bằng cách sử dụng chủng Tetrahymena môi trường làm sinh vật mô hình. Hoạt tính diệt động vật nguyên sinh của các chất tương tự được phát hiện phụ thuộc rất nhiều vào nhóm 4-hydroxyl ở vòng 6-aryl và chất thế 1-phenylethanamine ở vị trí 4. Hơn nữa, hiệu lực bị ảnh hưởng bởi kiểu thế thơm của phenylethanamine: các dẫn xuất thế không, meta-flo và para-brom có nồng độ diệt động vật nguyên sinh tối thiểu thấp nhất (8-16 μg/mL). Đáng ngạc nhiên là cả hai đồng phân đều có hiệu lực cao, điều này cho thấy lớp hợp chất này có thể có một số cơ chế hoạt động. Không tìm thấy mối tương quan nào giữa hoạt tính diệt động vật nguyên sinh của các hợp chất và hiệu lực ức chế tyrosine kinase của thụ thể yếu tố tăng trưởng biểu bì trong ống nghiệm. Điều này cho thấy các tác dụng kháng khuẩn quan sát được có liên quan đến các mục tiêu khác. Thử nghiệm đối với một nhóm kinase cho thấy một số cơ chế hoạt động thay thế."} +{"idx": 1990042, "en": "During cell division, the molecular motor Eg5 crosslinks overlapping antiparallel microtubules and pushes them apart to separate mitotic spindle poles. Dynein has been proposed as a direct antagonist of Eg5 at the spindle equator, pulling on antiparallel microtubules and favoring spindle collapse. Some of the experiments supporting this hypothesis relied on endpoint quantifications of spindle phenotypes rather than following individual cell fates over time. Here, we present a mathematical model and proof-of-principle experiments to demonstrate that endpoint quantifications can be fundamentally misleading because they overestimate defective phenotypes. Indeed, live-cell imaging reveals that, while depletion of dynein or the dynein binding protein Lis1 enables spindle formation in presence of an Eg5 inhibitor, the activities of dynein and Eg5 cannot be titrated against each other. Thus, dynein most likely antagonizes Eg5 indirectly by exerting force at different spindle locations rather than through a simple push-pull mechanism at the spindle equator.", "gemini": "Trong quá trình phân chia tế bào, động cơ phân tử Eg5 liên kết ngang các vi ống song song ngược chiều và đẩy chúng ra xa nhau để tách các cực của thoi vô sắc. Dynein đã được đề xuất là một chất đối kháng trực tiếp của Eg5 tại xích đạo của thoi, kéo các vi ống song song ngược chiều và tạo điều kiện cho thoi sụp đổ. Một số thí nghiệm hỗ trợ cho giả thuyết này dựa vào định lượng điểm cuối của kiểu hình thoi thay vì theo dõi số phận của từng tế bào theo thời gian. Ở đây, chúng tôi trình bày một mô hình toán học và các thí nghiệm chứng minh nguyên lý để chứng minh rằng định lượng điểm cuối có thể gây hiểu lầm về cơ bản vì chúng đánh giá quá cao các kiểu hình khiếm khuyết. Thật vậy, hình ảnh tế bào sống cho thấy rằng, trong khi sự cạn kiệt dynein hoặc protein liên kết dynein Lis1 cho phép hình thành thoi khi có chất ức chế Eg5, các hoạt động của dynein và Eg5 không thể được chuẩn độ với nhau. Do đó, dynein rất có thể đối kháng với Eg5 một cách gián tiếp bằng cách tác động lực tại các vị trí khác nhau của thoi chứ không phải thông qua cơ chế đẩy kéo đơn giản ở xích đạo của thoi."} +{"idx": 1990043, "en": "The cell wall is a defining structural feature of the bacterial subkingdom. However, most bacteria are capable of mutating into a cell-wall-deficient \"L-form\" state, requiring remarkable physiological and structural adaptations. L-forms proliferate by an unusual membrane deformation and scission process that is independent of the conserved and normally essential FtsZ based division machinery, and which may provide a model for the replication of primitive cells. Candidate gene screening revealed no requirement for the cytoskeletal systems that might actively drive membrane deformation or scission. Instead, we uncovered a crucial role for branched-chain fatty acid (BCFA) synthesis. BCFA-deficient mutants grow and undergo pulsating shape changes, but membrane scission fails, abolishing the separation of progeny cells. The failure in scission is associated with a reduction in membrane fluidity. The results identify a step in L-form proliferation and demonstrate that purely biophysical processes may have been sufficient for proliferation of primitive cells.", "gemini": "Thành tế bào là một đặc điểm cấu trúc xác định của phân ngành vi khuẩn. Tuy nhiên, hầu hết các vi khuẩn đều có khả năng đột biến thành trạng thái \"hình chữ L\" thiếu thành tế bào, đòi hỏi phải có những thích nghi sinh lý và cấu trúc đáng kể. Các dạng chữ L sinh sôi bằng một quá trình biến dạng và phân hạch màng bất thường, không phụ thuộc vào bộ máy phân chia dựa trên FtsZ được bảo tồn và thường thiết yếu, và có thể cung cấp một mô hình cho sự sao chép của các tế bào nguyên thủy. Sàng lọc gen ứng viên cho thấy không yêu cầu các hệ thống khung xương tế bào có thể chủ động thúc đẩy biến dạng hoặc phân hạch màng. Thay vào đó, chúng tôi phát hiện ra một vai trò quan trọng đối với quá trình tổng hợp axit béo mạch nhánh (BCFA). Các đột biến thiếu BCFA phát triển và trải qua những thay đổi hình dạng theo xung, nhưng sự phân hạch màng không thành công, làm mất sự tách biệt của các tế bào con. Sự thất bại trong phân hạch có liên quan đến việc giảm tính lưu động của màng. Kết quả xác định một bước trong quá trình tăng sinh dạng chữ L và chứng minh rằng các quá trình hoàn toàn sinh học có thể đủ cho sự tăng sinh của các tế bào nguyên thủy."} +{"idx": 1990044, "en": "To study the CD8(+) T cell response against a mouse γ-herpes virus, we generated K(b)-MHV-68-ORF8(604-612)RAG(-/-) CD8(+) T cell receptor transnuclear (TN) mice as a source of virus-specific CD8(+) T cells. K(b)-ORF8-Tet(+) CD8(+) T cells, expanded in the course of a resolving MHV-68 infection, served as a source of nucleus donors. Various in vivo and ex vivo assay criteria demonstrated the fine specificity and functionality of TN cells. TN cells proliferated extensively in response to viral infection, helped control viral burden, and exhibited a phenotype similar to that of endogenous K(b)-ORF8-Tet(+) cells. When compared to OT-1 cells, TN cells displayed distinct properties in response to lymphopenia and cognate antigen stimulation, which may be attributable to the affinity of the TCR expressed by the TN cells. The availability of MHV-68-specific CD8(+) TCR TN mice provides a new tool for investigating aspects of host-pathogen interactions unique to γ-herpes viruses.", "gemini": "Để nghiên cứu phản ứng của tế bào T CD8(+) chống lại vi-rút γ-herpes ở chuột, chúng tôi đã tạo ra chuột thụ thể tế bào T CD8(+) K(b)-MHV-68-ORF8(604-612)RAG(-/-) chuyển nhân (TN) làm nguồn tế bào T CD8(+) đặc hiệu với vi-rút. Tế bào T CD8(+) K(b)-ORF8-Tet(+), được mở rộng trong quá trình nhiễm MHV-68 đang giải quyết, đóng vai trò là nguồn cung cấp các tế bào cho nhân. Nhiều tiêu chí đánh giá in vivo và ex vivo đã chứng minh tính đặc hiệu và chức năng tốt của các tế bào TN. Các tế bào TN tăng sinh mạnh mẽ để đáp ứng với nhiễm trùng vi-rút, giúp kiểm soát gánh nặng vi-rút và biểu hiện kiểu hình tương tự như các tế bào K(b)-ORF8-Tet(+) nội sinh. Khi so sánh với các tế bào OT-1, các tế bào TN thể hiện các đặc tính riêng biệt để đáp ứng với tình trạng giảm bạch cầu và kích thích kháng nguyên nhận biết, có thể là do ái lực của TCR được các tế bào TN biểu hiện. Sự sẵn có của chuột TN TCR CD8(+) đặc hiệu MHV-68 cung cấp một công cụ mới để nghiên cứu các khía cạnh của tương tác vật chủ-bệnh nguyên độc đáo đối với vi-rút γ-herpes."} +{"idx": 1990045, "en": "mRNAs encoding polarity and secretion factors (POLs) target the incipient bud site in yeast for localized translation during division. In pheromone-treated cells we now find that these mRNAs are also localized to the yeast-mating projection (shmoo) tip. However, in contrast to the budding program, neither the She2 nor She3 proteins are involved. Instead, the Scp160 RNA-binding protein binds POL and mating pathway mRNAs and regulates their spatial distribution in a Myo4- and cortical ER-dependent fashion. RNA binding by Scp160 is stimulated by activation of Gpa1, the G protein α subunit regulated by the pheromone receptor, and is required for pheromone gradient sensing, as well as subsequent chemotropic growth and cell-cell mating. These effects are incurred independently of obvious changes in translation; thus, mRNA trafficking is required for chemotropism and completion of the mating program. This is, to our knowledge, the first demonstration of ligand-activated RNA targeting in the development of a simple eukaryote.", "gemini": "Các mRNA mã hóa các yếu tố phân cực và tiết (POL) nhắm vào vị trí nảy chồi mới hình thành trong nấm men để dịch cục bộ trong quá trình phân chia. Trong các tế bào được xử lý bằng pheromone, chúng tôi hiện thấy rằng các mRNA này cũng được định vị ở đầu mấu giao phối của nấm men (shmoo). Tuy nhiên, trái ngược với chương trình nảy chồi, không có protein She2 hoặc She3 nào tham gia. Thay vào đó, protein liên kết RNA Scp160 liên kết với POL và các mRNA của con đường giao phối và điều chỉnh sự phân bố không gian của chúng theo kiểu phụ thuộc vào Myo4 và ER vỏ não. Liên kết RNA của Scp160 được kích thích bởi sự hoạt hóa của Gpa1, tiểu đơn vị α của protein G được điều chỉnh bởi thụ thể pheromone, và là cần thiết để cảm nhận gradient pheromone, cũng như sự phát triển hướng hóa học và giao phối tế bào-tế bào tiếp theo. Những tác động này xảy ra độc lập với những thay đổi rõ ràng trong quá trình dịch; do đó, vận chuyển mRNA là cần thiết cho hướng hóa học và hoàn thành chương trình giao phối. Theo hiểu biết của chúng tôi, đây là minh chứng đầu tiên về sự nhắm mục tiêu RNA được kích hoạt bởi phối tử trong quá trình phát triển của một sinh vật nhân chuẩn đơn giản."} +{"idx": 1990046, "en": "Most vertebrate embryos break symmetry by a cilia-driven leftward flow during neurulation. In the frog Xenopus asymmetric expression of the ion pump ATP4a was reported at the 4-cell stage. The \"ion-flux\" model postulates that symmetry is broken flow-independently through an ATP4-generated asymmetric voltage gradient that drives serotonin through gap junctions to one side of the embryo. Here, we show that ATP4a is symmetrically expressed. Gene knockdown or pharmacological inhibition compromised organ situs, asymmetric marker gene expression, and leftward flow. The gastrocoel roof plate (GRP), where flow in frog occurs, revealed fewer, shortened, and misaligned cilia. Foxj1, a master control gene of motile cilia, was downregulated in the superficial mesoderm, from which the GRP develops. Specifically, ATP4 was required for Wnt/β-catenin-regulated Foxj1 induction and Wnt/PCP-dependent cilia polarization. Our work argues for evolutionary conservation of symmetry breakage in the vertebrates.", "gemini": "Hầu hết các phôi động vật có xương sống phá vỡ tính đối xứng bằng một dòng chảy lệch về bên trái do lông mao trong quá trình tạo thành ống thần kinh. Trong ếch Xenopus, biểu hiện không đối xứng của bơm ion ATP4a đã được báo cáo ở giai đoạn 4 tế bào. Mô hình \"dòng ion\" giả định rằng tính đối xứng bị phá vỡ một cách độc lập với dòng chảy thông qua một gradient điện thế không đối xứng do ATP4 tạo ra, thúc đẩy serotonin đi qua các mối nối khe đến một bên của phôi. Ở đây, chúng tôi chỉ ra rằng ATP4a được biểu hiện đối xứng. Việc hạ gục gen hoặc ức chế dược lý làm tổn hại đến vị trí cơ quan, biểu hiện gen đánh dấu không đối xứng và dòng chảy lệch về bên trái. Mái vòm tấm mái dạ dày (GRP), nơi dòng chảy ở ếch xảy ra, cho thấy ít lông mao hơn, ngắn hơn và không thẳng hàng. Foxj1, một gen kiểm soát chính của lông mao vận động, bị giảm điều hòa ở trung mô bề mặt, nơi phát triển GRP. Cụ thể, ATP4 là cần thiết cho sự cảm ứng Foxj1 được điều chỉnh bởi Wnt/β-catenin và phân cực lông mao phụ thuộc Wnt/PCP. Công trình của chúng tôi lập luận cho sự bảo tồn tiến hóa của sự phá vỡ tính đối xứng ở động vật có xương sống."} +{"idx": 1990047, "en": "Synaptic NMDA receptors (NMDARs) are crucial for neural coding and plasticity. However, little is known about the adaptive function of extrasynaptic NMDARs occurring mainly on dendritic shafts. Here, we find that in CA1 pyramidal neurons, back-propagating action potentials (bAPs) recruit shaft NMDARs exposed to ambient glutamate. In contrast, spine NMDARs are \"protected,\" under baseline conditions, from such glutamate influences by peri-synaptic transporters: we detect bAP-evoked Ca(2+) entry through these receptors upon local synaptic or photolytic glutamate release. During theta-burst firing, NMDAR-dependent Ca(2+) entry either downregulates or upregulates an h-channel conductance (G(h)) of the cell depending on whether synaptic glutamate release is intact or blocked. Thus, the balance between activation of synaptic and extrasynaptic NMDARs can determine the sign of G(h) plasticity. G(h) plasticity in turn regulates dendritic input probed by local glutamate uncaging. These results uncover a metaplasticity mechanism potentially important for neural coding and memory formation.", "gemini": "Các thụ thể NMDA (NMDAR) ở synap rất quan trọng đối với mã hóa và tính dẻo của nơ-ron. Tuy nhiên, người ta biết rất ít về chức năng thích nghi của các NMDAR ngoài synap chủ yếu xảy ra trên thân cây phát triển. Ở đây, chúng tôi thấy rằng ở các tế bào thần kinh hình chóp CA1, các điện thế hoạt động ngược dòng (bAP) tuyển dụng các NMDAR thân cây tiếp xúc với glutamate xung quanh. Ngược lại, các NMDAR gai \"được bảo vệ\", trong điều kiện cơ bản, khỏi những ảnh hưởng của glutamate như vậy bởi các chất vận chuyển quanh synap: chúng tôi phát hiện thấy sự xâm nhập Ca(2+) do bAP gây ra thông qua các thụ thể này khi giải phóng glutamate tại chỗ ở synap hoặc quang phân. Trong quá trình bắn xung theo nhịp theta, sự xâm nhập Ca(2+) phụ thuộc NMDAR hoặc làm giảm hoặc tăng cường độ dẫn điện của kênh h (G(h)) của tế bào tùy thuộc vào việc giải phóng glutamate ở synap còn nguyên vẹn hay bị chặn. Do đó, sự cân bằng giữa sự hoạt hóa của các NMDAR synap và ngoài synap có thể xác định dấu hiệu của tính dẻo G(h). Tính dẻo của G(h) lần lượt điều chỉnh đầu vào của phát triển được thăm dò bằng cách giải phóng glutamate tại chỗ. Những kết quả này khám phá ra một cơ chế siêu dẻo có khả năng quan trọng đối với mã hóa nơ-ron và hình thành trí nhớ."} +{"idx": 1990048, "en": "Developing organisms have evolved a wide range of mechanisms for coping with recurrent environmental challenges. How they cope with rare or unforeseen challenges is, however, unclear as are the implications to their unchallenged offspring. Here, we investigate these questions by confronting the development of the fly, D. melanogaster, with artificial tissue distributions of toxic stress that are not expected to occur during fly development. We show that under a wide range of toxic scenarios, this challenge can lead to modified development that may coincide with increased tolerance to an otherwise lethal condition. Part of this response was mediated by suppression of Polycomb group genes, which in turn leads to derepression of developmental regulators and their expression in new domains. Importantly, some of the developmental alterations were epigenetically inherited by subsequent generations of unchallenged offspring. These results show that the environment can induce alternative patterns of development that are stable across multiple generations.", "gemini": "Các sinh vật đang phát triển đã tiến hóa một loạt các cơ chế để đối phó với những thách thức tái diễn của môi trường. Tuy nhiên, cách chúng đối phó với những thách thức hiếm gặp hoặc không lường trước được thì vẫn chưa rõ ràng cũng như những tác động đến thế hệ con cháu không bị thách thức của chúng. Ở đây, chúng tôi nghiên cứu những câu hỏi này bằng cách đối đầu với sự phát triển của loài ruồi giấm, D. melanogaster, với sự phân bố mô nhân tạo của căng thẳng độc hại mà không được dự kiến sẽ xảy ra trong quá trình phát triển của ruồi. Chúng tôi chỉ ra rằng trong nhiều trường hợp ngộ độc, thách thức này có thể dẫn đến sự phát triển bị thay đổi có thể trùng hợp với khả năng chịu đựng tăng lên đối với một tình trạng gây tử vong. Một phần của phản ứng này được trung gian bởi sự ức chế các gen nhóm Polycomb, từ đó dẫn đến sự giải ức chế các chất điều hòa phát triển và sự biểu hiện của chúng trong các miền mới. Điều quan trọng là một số thay đổi về phát triển đã được di truyền theo kiểu biểu sinh bởi các thế hệ con cháu không bị thách thức tiếp theo. Những kết quả này cho thấy rằng môi trường có thể gây ra các kiểu phát triển thay thế ổn định qua nhiều thế hệ."} +{"idx": 1990049, "en": "During evolution segments of homeothermic genomes underwent a GC content increase. Our analyses reveal that two exon-intron architectures have evolved from an ancestral state of low GC content exons flanked by short introns with a lower GC content. One group underwent a GC content elevation that abolished the differential exon-intron GC content, with introns remaining short. The other group retained the overall low GC content as well as the differential exon-intron GC content, and is associated with longer introns. We show that differential exon-intron GC content regulates exon inclusion level in this group, in which disease-associated mutations often lead to exon skipping. This group's exons also display higher nucleosome occupancy compared to flanking introns and exons of the other group, thus \"marking\" them for spliceosomal recognition. Collectively, our results reveal that differential exon-intron GC content is a previously unidentified determinant of exon selection and argue that the two GC content architectures reflect the two mechanisms by which splicing signals are recognized: exon definition and intron definition.", "gemini": "Trong quá trình tiến hóa, các đoạn của bộ gen đẳng nhiệt đã trải qua sự gia tăng hàm lượng GC. Các phân tích của chúng tôi tiết lộ rằng hai cấu trúc exon-intron đã tiến hóa từ trạng thái tổ tiên của các exon có hàm lượng GC thấp được bao bọc bởi các intron ngắn có hàm lượng GC thấp hơn. Một nhóm đã trải qua sự gia tăng hàm lượng GC đã xóa bỏ hàm lượng GC exon-intron khác biệt, với các intron vẫn còn ngắn. Nhóm còn lại vẫn giữ hàm lượng GC thấp tổng thể cũng như hàm lượng GC exon-intron khác biệt và có liên quan đến các intron dài hơn. Chúng tôi chỉ ra rằng hàm lượng GC exon-intron khác biệt điều chỉnh mức độ đưa vào exon trong nhóm này, trong đó các đột biến liên quan đến bệnh thường dẫn đến việc bỏ qua exon. Các exon của nhóm này cũng biểu hiện mức độ chiếm giữ nucleosome cao hơn so với các intron bao quanh và các exon của nhóm kia, do đó \"đánh dấu\" chúng để nhận dạng bằng spliceosomal. Nhìn chung, kết quả của chúng tôi cho thấy rằng hàm lượng GC exon-intron khác biệt là một yếu tố xác định chưa từng được biết đến trước đây đối với sự lựa chọn exon và lập luận rằng hai cấu trúc hàm lượng GC phản ánh hai cơ chế mà tín hiệu ghép nối được nhận dạng: định nghĩa exon và định nghĩa intron."} +{"idx": 1990050, "en": "A small-sized sonochemical reactor in which the absolute value of the sound pressure amplitude can be estimated from the vibration velocity of the transducer was investigated. The sound pressure distribution in the reactor and the relationship between the vibration velocity and the sound pressure amplitude were derived through Helmholtz wave equation. The reactor consists of a bolt-clamped Langevin transducer and a rectangular cell with a tungsten reflector. A 3λ/4-standing-wave-field was generated in the reactor to simplify the sound pressure distribution. The sound pressure distribution was measured from the optical refractive index change of water using a laser interferometer. The experimental and theoretical results showed a good agreement in the absolute value of the sound pressure amplitude, and it was confirmed that the sound pressure in the sonochemical reactor can be estimated from the input current of the vibrator.", "gemini": "Một lò phản ứng sóng siêu âm có kích thước nhỏ trong đó giá trị tuyệt đối của biên độ áp suất âm có thể được ước tính từ vận tốc rung của đầu dò đã được nghiên cứu. Sự phân bố áp suất âm trong lò phản ứng và mối quan hệ giữa vận tốc rung và biên độ áp suất âm đã được suy ra thông qua phương trình sóng Helmholtz. Lò phản ứng bao gồm một đầu dò Langevin kẹp bu lông và một ô hình chữ nhật có phản xạ vonfram. Một trường sóng dừng 3λ/4 đã được tạo ra trong lò phản ứng để đơn giản hóa sự phân bố áp suất âm. Sự phân bố áp suất âm được đo từ sự thay đổi chiết suất quang của nước bằng cách sử dụng giao thoa kế laser. Kết quả thực nghiệm và lý thuyết cho thấy sự phù hợp tốt về giá trị tuyệt đối của biên độ áp suất âm và đã xác nhận rằng áp suất âm trong lò phản ứng sóng siêu âm có thể được ước tính từ dòng điện đầu vào của máy rung."} +{"idx": 1990051, "en": "OBJECTIVE Breast cancer is a heterogeneous neoplasm. Distinct subtypes of breast cancer have been defined, suggesting the existence of molecular differences contributing to their clinical outcomes. However, the molecular differences between human epidermal growth factor receptor 2 (HER2)-positive and HER2-negative breast cancer tumors remain unclear. The aim of this study was to identify a gene expression profile for breast tumors based on their HER2 status. METHODS The HER2 status was determined by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) in 54 breast tumor samples. Using Affymetrix microarray data from these breast tumors, we established the expression profiling of breast cancer based on HER2 IHC and FISH results. To validate microarray experiment data, real-time quantitative reverse transcription-PCR was performed. RESULTS We found significant differences between the HER2-positive and HER2-negative breast tumor samples, which included overexpression of HER2, other genes located on 17q12 and genes functionally related to migration. CONCLUSION Our study shows the potential of integrated genomic profiling to shed light on the molecular knowledge of HER2-positive breast tumors.", "gemini": "MỤC TIÊU Ung thư vú là một khối u ác tính không đồng nhất. Các phân nhóm ung thư vú riêng biệt đã được xác định, cho thấy sự tồn tại của các khác biệt về phân tử góp phần vào kết quả lâm sàng của chúng. Tuy nhiên, sự khác biệt về phân tử giữa các khối u ung thư vú dương tính với thụ thể yếu tố tăng trưởng biểu bì ở người 2 (HER2) và âm tính với HER2 vẫn chưa rõ ràng. Mục đích của nghiên cứu này là xác định hồ sơ biểu hiện gen của các khối u vú dựa trên tình trạng HER2 của chúng. PHƯƠNG PHÁP Tình trạng HER2 được xác định bằng phương pháp miễn dịch mô hóa học (IHC) và lai tại chỗ huỳnh quang (FISH) trong 54 mẫu khối u vú. Sử dụng dữ liệu mảng vi mô Affymetrix từ các khối u vú này, chúng tôi đã thiết lập hồ sơ biểu hiện của ung thư vú dựa trên kết quả IHC và FISH của HER2. Để xác nhận dữ liệu thí nghiệm mảng vi mô, phản ứng chuỗi phiên mã ngược định lượng thời gian thực đã được thực hiện. KẾT QUẢ Chúng tôi đã tìm thấy sự khác biệt đáng kể giữa các mẫu khối u vú dương tính với HER2 và âm tính với HER2, bao gồm sự biểu hiện quá mức của HER2, các gen khác nằm trên 17q12 và các gen có liên quan chức năng đến di chuyển. KẾT LUẬN Nghiên cứu của chúng tôi cho thấy tiềm năng của hồ sơ giải trình tự gen tích hợp để làm sáng tỏ kiến thức phân tử về các khối u vú dương tính với HER2."} +{"idx": 1990052, "en": "BACKGROUND The substituted cathinone compound known as mephedrone (4-methylmethcathinone; 4-MMC) has become popular with recreational users of psychomotor-stimulant compounds. Only recently have the first preclinical studies provided information about this drug in the scientific literature; nevertheless, media reports have led to drug control actions in the UK and across several US states. Rodent studies indicate that 4-MMC exhibits neuropharmacological similarity to 3,4-methylenedioxymethamphetamine (MDMA) and prompt investigation of the thermoregulatory, cardiac and locomotor effects of 4-MMC. This study focuses on the role of ambient temperature, which has been shown to shift the effects of MDMA from hyperthermic to hypothermic. METHODS Male Sprague-Dawley rats were monitored after subcutaneous administration of 4-MMC (1.0-5.6 mg/kg) using an implantable radiotelemetry system under conditions of low (20 °C) and high (30 °C) ambient temperature. RESULTS A pharmacokinetic study found a T(max) of 0.25 h and a C(max) of 1206 ng/ml after 5.6 mg/kg 4-MMC. A dose-dependent reduction of body temperature was produced by 4-MMC at 20 °C but there was no temperature change at 30 °C. Increased locomotor activity was observed after 4-MMC administration under both ambient temperatures, however, significantly more activity was observed at 30 °C. Heart rate was slowed by 1.0 and 5.6 mg/kg 4-MMC at 20°C, and was slower in the 30 °C vs. 20 °C condition across all treatments. CONCLUSION These results show that the cathinone analog 4-MMC exhibits in vivo thermoregulatory properties that are distinct from those produced by MDMA.", "gemini": "BỐI CẢNH Hợp chất cathinone thay thế được gọi là mephedrone (4-methylmethcathinone; 4-MMC) đã trở nên phổ biến với những người sử dụng các hợp chất kích thích tâm vận động vì mục đích giải trí. Chỉ mới gần đây, các nghiên cứu tiền lâm sàng đầu tiên đã cung cấp thông tin về loại thuốc này trong tài liệu khoa học; tuy nhiên, các báo cáo của phương tiện truyền thông đã dẫn đến các hành động kiểm soát thuốc ở Vương quốc Anh và trên nhiều tiểu bang của Hoa Kỳ. Các nghiên cứu trên loài gặm nhấm chỉ ra rằng 4-MMC thể hiện sự tương đồng về dược lý thần kinh với 3,4-methylenedioxymethamphetamine (MDMA) và thúc đẩy quá trình điều tra về tác dụng điều hòa nhiệt, tim mạch và vận động của 4-MMC. Nghiên cứu này tập trung vào vai trò của nhiệt độ môi trường, đã được chứng minh là làm thay đổi tác dụng của MDMA từ tăng thân nhiệt sang hạ thân nhiệt. PHƯƠNG PHÁP Chuột Sprague-Dawley đực được theo dõi sau khi tiêm dưới da 4-MMC (1,0-5,6 mg/kg) bằng hệ thống đo từ xa cấy ghép trong điều kiện nhiệt độ môi trường thấp (20 °C) và cao (30 °C). KẾT QUẢ Một nghiên cứu dược động học cho thấy T(max) là 0,25 giờ và C(max) là 1206 ng/ml sau khi dùng 5,6 mg/kg 4-MMC. 4-MMC tạo ra sự giảm nhiệt độ cơ thể phụ thuộc vào liều ở 20 °C nhưng không có sự thay đổi nhiệt độ ở 30 °C. Hoạt động vận động tăng lên đã được quan sát thấy sau khi dùng 4-MMC ở cả hai nhiệt độ môi trường, tuy nhiên, hoạt động nhiều hơn đáng kể ở 30 °C. Nhịp tim chậm lại do 1,0 và 5,6 mg/kg 4-MMC ở 20 °C và chậm hơn ở điều kiện 30 °C so với 20 °C trên tất cả các phương pháp điều trị. KẾT LUẬN Các kết quả này cho thấy chất tương tự cathinone 4-MMC thể hiện các đặc tính điều hòa nhiệt trong cơ thể khác biệt với các đặc tính do MDMA tạo ra."} +{"idx": 1990053, "en": "An estimated 25 million people in the US alone rely on implanted medical devices, ∼2.5 million implanted within the nervous system. Even though many devices perform adequately for years, the host response to medical devices often severely limits tissue integration and long-term performance. This host response is believed to be particularly limiting in the case of intracortical microelectrodes, where it has been shown that glial cell encapsulation and localized neuronal cell loss accompany intracortical microelectrode implantation. Since neuronal ensembles must be within ∼50 µm of the electrode to obtain neuronal spikes and local field potentials, developing a better understanding of the molecular and cellular environment at the device-tissue interface has been the subject of significant research. Unfortunately, immunohistochemical studies of scar maturation in correlation to device function have been inconclusive. Therefore, here we present a detailed quantitative study of the cellular events and the stability of the blood-brain barrier (BBB) following intracortical microelectrode implantation and cortical stab injury in a chronic survival model. We found two distinctly inverse multiphasic profiles for neuronal survival in device-implanted tissue compared to stab-injured animals. For chronically implanted animals, we observed a biphasic paradigm between blood-derived/trauma-induced and CNS-derived inflammatory markers driving neurodegeneration at the interface. In contrast, stab injured animals demonstrated a CNS-mediated neurodegenerative environment. Collectively these data provide valuable insight to the possibility of multiple roles of chronic neuroinflammatory events on BBB disruption and localized neurodegeneration, while also suggesting the importance to consider multiphasic neuroinflammatory kinetics in the design of therapeutic strategies for stabilizing neural interfaces.", "gemini": "Ước tính chỉ riêng tại Hoa Kỳ đã có 25 triệu người dựa vào các thiết bị y tế cấy ghép, ∼2,5 triệu thiết bị được cấy ghép trong hệ thần kinh. Mặc dù nhiều thiết bị hoạt động tốt trong nhiều năm, nhưng phản ứng của vật chủ đối với các thiết bị y tế thường hạn chế nghiêm trọng sự tích hợp của mô và hiệu suất lâu dài. Người ta tin rằng phản ứng của vật chủ này đặc biệt hạn chế trong trường hợp các vi điện cực nội sọ, nơi đã chứng minh rằng sự bao bọc của tế bào thần kinh đệm và mất tế bào thần kinh cục bộ đi kèm với việc cấy ghép vi điện cực nội sọ. Vì các nhóm tế bào thần kinh phải cách điện cực ∼50 µm để thu được các xung thần kinh và điện thế trường địa phương, nên việc hiểu rõ hơn về môi trường phân tử và tế bào tại giao diện thiết bị-mô đã trở thành chủ đề của nhiều nghiên cứu quan trọng. Thật không may, các nghiên cứu miễn dịch mô hóa học về quá trình sẹo trưởng thành có liên quan đến chức năng của thiết bị vẫn chưa đưa ra kết luận. Do đó, tại đây chúng tôi trình bày một nghiên cứu định lượng chi tiết về các sự kiện tế bào và sự ổn định của hàng rào máu não (BBB) sau khi cấy ghép vi điện cực nội sọ và chấn thương đâm vỏ não trong mô hình sống sót mạn tính. Chúng tôi đã tìm thấy hai hồ sơ đa pha ngược nhau rõ rệt về khả năng sống sót của tế bào thần kinh ở mô cấy ghép thiết bị so với động vật bị thương do đâm. Đối với động vật được cấy ghép mạn tính, chúng tôi đã quan sát thấy một mô hình hai pha giữa các dấu hiệu viêm do chấn thương/do máu và các dấu hiệu viêm có nguồn gốc từ CNS thúc đẩy thoái hóa thần kinh tại giao diện. Ngược lại, động vật bị thương do đâm đã chứng minh một môi trường thoái hóa thần kinh do CNS. Cùng nhau, những dữ liệu này cung cấp cái nhìn sâu sắc có giá trị về khả năng xảy ra nhiều vai trò của các sự kiện viêm thần kinh mạn tính đối với sự phá vỡ BBB và thoái hóa thần kinh cục bộ, đồng thời cũng gợi ý tầm quan trọng của việc xem xét động học viêm thần kinh đa pha trong thiết kế các chiến lược điều trị để ổn định các giao diện thần kinh."} +{"idx": 1990054, "en": "Both chronic subdural hematoma and arachnoid cysts are common lesions in neurosurgical practice. Arachnoid cysts are a well-known predisposing factor for chronic subdural hematoma. Here, we present a 12-year-old taekwondo athlete with chronic subdural hematoma associated with arachnoid cysts. The chronic subdural hematoma was evacuated through 2 burr holes and the patient was discharged in good condition. To our knowledge, this is the first case of chronic subdural hematoma with associated arachnoid cysts in a taekwondo athlete. We also review the literature on sports-related chronic subdural hematomas associated with arachnoid cysts in children.", "gemini": "Cả tụ máu dưới màng cứng mạn tính và nang nhện đều là những tổn thương thường gặp trong thực hành phẫu thuật thần kinh. Nang nhện là một yếu tố thuận lợi gây tụ máu dưới màng cứng mạn tính. Ở đây, chúng tôi trình bày một vận động viên taekwondo 12 tuổi mắc tụ máu dưới màng cứng mạn tính liên quan đến nang nhện. Tụ máu dưới màng cứng mạn tính đã được dẫn lưu qua 2 lỗ khoan và bệnh nhân được xuất viện trong tình trạng tốt. Theo chúng tôi biết, đây là trường hợp đầu tiên bị tụ máu dưới màng cứng mạn tính liên quan đến nang nhện ở một vận động viên taekwondo. Chúng tôi cũng xem xét các tài liệu về tụ máu dưới màng cứng mạn tính liên quan đến thể thao liên quan đến nang nhện ở trẻ em."} +{"idx": 1990055, "en": "BACKGROUND Patients with calcium pyrophosphate deposition disease sometimes require a continuous therapy. Nonsteroidal anti-inflammatory drugs, colchicine, and glucocorticoids are commonly used with good results, but occasionally, these are ineffective, contraindicated, or not tolerated. We present our experience with methotrexate (MTX) in refractory CPP arthritis. METHODS An observational study to describe and evaluate the use of MTX in refractory calcium pyrophosphate deposition disease was undertaken. Ten patients were included. Treatment response was evaluated by the physician (excellent, good, medium, poor, or absent response) and the patient as measured on a 10-cm visual analog scale (0 = no effect; 10 = complete resolution of symptoms). Adverse effects were recorded on all patients. RESULTS Five patients presented a persistent polyarthritis, 3 presented a persistent oligoarthritis, and 2 presented a very frequently recurring monoarthritis. Median MTX evaluation by patients on visual analog scale was 7.4, and physicians considered excellent (n = 2), good (n = 5), or medium (n = 3). The safety profile was generally good, but MTX was discontinued in 2 patients because of adverse effects (transient bone marrow aplasia and elevation of liver enzymes). CONCLUSIONS Methotrexate could be an effective and safe option for patients with refractory CPP arthritis, but additional studies are necessary to determine to what extent and for which patients MTX is effective.", "gemini": "BỐI CẢNH Bệnh nhân mắc bệnh lắng đọng pyrophosphate canxi đôi khi cần phải điều trị liên tục. Thuốc chống viêm không steroid, colchicine và glucocorticoid thường được sử dụng với kết quả tốt, nhưng đôi khi, những thuốc này không hiệu quả, chống chỉ định hoặc không dung nạp. Chúng tôi trình bày kinh nghiệm của mình với methotrexate (MTX) trong viêm khớp CPP khó chữa. PHƯƠNG PHÁP Một nghiên cứu quan sát để mô tả và đánh giá việc sử dụng MTX trong bệnh lắng đọng pyrophosphate canxi khó chữa đã được tiến hành. Mười bệnh nhân đã được đưa vào. Đáp ứng điều trị được bác sĩ đánh giá (tuyệt vời, tốt, trung bình, kém hoặc không đáp ứng) và bệnh nhân đánh giá trên thang tương tự thị giác 10 cm (0 = không có tác dụng; 10 = hết hoàn toàn các triệu chứng). Tác dụng phụ được ghi nhận ở tất cả bệnh nhân. KẾT QUẢ Năm bệnh nhân bị viêm đa khớp dai dẳng, 3 bệnh nhân bị viêm ít khớp dai dẳng và 2 bệnh nhân bị viêm một khớp tái phát rất thường xuyên. Đánh giá MTX trung bình của bệnh nhân trên thang tương tự thị giác là 7,4 và các bác sĩ coi là tuyệt vời (n = 2), tốt (n = 5) hoặc trung bình (n = 3). Hồ sơ an toàn nhìn chung là tốt, nhưng MTX đã bị ngừng ở 2 bệnh nhân do tác dụng phụ (thiếu máu cục bộ xương tạm thời và tăng men gan). KẾT LUẬN Methotrexate có thể là một lựa chọn hiệu quả và an toàn cho những bệnh nhân bị viêm khớp CPP khó chữa, nhưng cần có thêm các nghiên cứu để xác định mức độ hiệu quả của MTX đối với những bệnh nhân nào."} +{"idx": 1990056, "en": "A 54-year-old woman with recurrent episodes of bilateral knee arthritis was admitted several times to the Department of Rheumatology. She was diagnosed to have chronic calcium pyrophosphate crystal arthritis. Interleukin 1β (IL-1β) plays a central role in the pathogenesis of inflammation induced by calcium pyrophosphate crystals, and IL-1β blockade may be an effective treatment in patients with severe chronic calcium pyrophosphate crystal arthritis. Because of the short effect of treatment with corticosteroids and the frequent attacks of arthritis, the patient was treated with the IL-1 receptor antagonist anakinra. She responded well after 1 week with normalization of inflammation. Eight months after the initiation of treatment, the patient has had no relapses, although we were unable to withdraw the anakinra.", "gemini": "Một phụ nữ 54 tuổi bị các đợt viêm khớp gối tái phát đã được nhập viện nhiều lần vào Khoa thấp khớp. Bà được chẩn đoán mắc bệnh viêm khớp tinh thể pyrophosphate canxi mạn tính. Interleukin 1β (IL-1β) đóng vai trò trung tâm trong quá trình sinh bệnh của tình trạng viêm do tinh thể pyrophosphate canxi gây ra và việc phong bế IL-1β có thể là một phương pháp điều trị hiệu quả ở những bệnh nhân bị viêm khớp tinh thể pyrophosphate canxi mạn tính nặng. Do tác dụng ngắn của việc điều trị bằng corticosteroid và các cơn viêm khớp thường xuyên, bệnh nhân đã được điều trị bằng chất đối kháng thụ thể IL-1 anakinra. Bà đã đáp ứng tốt sau 1 tuần với việc tình trạng viêm trở lại bình thường. Tám tháng sau khi bắt đầu điều trị, bệnh nhân không tái phát mặc dù chúng tôi không thể ngừng dùng anakinra."} +{"idx": 1990057, "en": "In Afghanistan in mid June 2011, 2 US Marines developed reactive arthritis manifested by conjunctivitis, urethritis, arthritis, and circinate balanitis. Their symptoms were preceded by an outbreak in their unit of gastroenteritis caused by Shigella species after ingesting contaminated chicken. Gastroenteritis has plagued military operations for millennia. This report emphasizes that personnel can develop reactive arthritis after bouts of gastrointestinal infections that are common in deployed environments. It is highly recommended to maintain vigilance in keeping reactive arthritis on the differential diagnosis in deployed personnel after bouts of gastroenteritis.", "gemini": "Vào giữa tháng 6 năm 2011 tại Afghanistan, 2 lính thủy đánh bộ Hoa Kỳ đã phát triển viêm khớp phản ứng biểu hiện bằng viêm kết mạc, viêm niệu đạo, viêm khớp và viêm quy đầu hình tròn. Các triệu chứng của họ trước đó là một đợt bùng phát viêm dạ dày ruột do vi khuẩn Shigella gây ra sau khi ăn phải thịt gà bị nhiễm bẩn. Viêm dạ dày ruột đã gây ra nhiều đau khổ cho các hoạt động quân sự trong hàng thiên niên kỷ. Báo cáo này nhấn mạnh rằng nhân viên có thể phát triển viêm khớp phản ứng sau các cơn nhiễm trùng đường tiêu hóa phổ biến trong môi trường triển khai. Rất nên duy trì cảnh giác trong việc giữ cho viêm khớp phản ứng trên chẩn đoán phân biệt ở những người triển khai sau các cơn viêm dạ dày ruột."} +{"idx": 1990058, "en": "Pachydermodactyly is a superficial benign fibromatosis of unknown etiology; it is rare, more frequent in adolescent males, and characterized by painless swelling of the proximal interphalangeal joints(PIP) of the hands. Histologic examination of the skin shows epidermal hyperplasia and increased number of dermal fibroblasts and collagen fibers.We report the case of a 16-year-old adolescent boy who presented swelling of the lateral and dorsal regions of all the metacarpophalangeal and PIP joints of the left hand and PIP and metacarpophalangeal joints of the second and fifth fingers of the right hand, with 3 years of evolution and no arthritis or functional impairment. Results of complementary diagnostic examinations were normal, with the exception of hand ultra sound that showed skin thickening, without synovial proliferation or joint effusion. Skin biopsy confirmed pachydermodactyly. The patient under went aesthetic surgery with good outcome, without recurrence.This rare condition should be distinguished from idiopathic juvenile arthritis and other entities such as knuckle pads syndrome.", "gemini": "Pachydermodactyly là một bệnh xơ hóa lành tính nông không rõ nguyên nhân; nó rất hiếm, thường gặp hơn ở nam thanh thiếu niên và đặc trưng bởi tình trạng sưng không đau ở các khớp liên ngón gần (PIP) của bàn tay. Kiểm tra mô học của da cho thấy tăng sản biểu bì và tăng số lượng nguyên bào sợi da và sợi collagen. Chúng tôi báo cáo trường hợp một bé trai vị thành niên 16 tuổi bị sưng ở vùng bên và vùng lưng của tất cả các khớp bàn ngón tay và PIP của bàn tay trái và các khớp PIP và khớp bàn ngón tay của ngón tay thứ hai và thứ năm của bàn tay phải, với 3 năm tiến triển và không bị viêm khớp hoặc suy giảm chức năng. Kết quả của các xét nghiệm chẩn đoán bổ sung là bình thường, ngoại trừ siêu âm bàn tay cho thấy da dày lên, không có sự tăng sinh màng hoạt dịch hoặc tràn dịch khớp. Sinh thiết da đã xác nhận pachydermodactyly. Bệnh nhân đã trải qua phẫu thuật thẩm mỹ với kết quả tốt, không tái phát. Tình trạng hiếm gặp này nên được phân biệt với viêm khớp tự phát ở trẻ em và các thực thể khác như hội chứng đệm khớp ngón tay."} +{"idx": 1990059, "en": "As is so often the case, a molecule gets named for its first identified activity or apparent role and then that initial name sticks, even as new and perhaps fundamentally different activities emerge from later studies. It is the special power of evolution that takes a certain activity and then uses it over and over again in pursuit of apparently disparate goals in a maturing or mature organism. In general terms, transforming growth factor β (TGF-β) is intimately involved in a variety of differentiation and growth inhibition processes, in apoptosis, and in deposition of the extracellular matrix. Initially identified in its role in oncogenesis, TGF-β is now implicated in a number of vascular and rheumatologic disorders, perhaps most notably the scleroderma. TGF-β has been identified as a powerful influence in angiogenesis, wound healing, joint inflammation, tumor growth and metastasis, and, of course, immunoregulation. So \"what is in a name?\" A rose by any other name would smell as sweet and would still be immunologically active, even if the name is \"misleading.\"", "gemini": "Như thường lệ, một phân tử được đặt tên theo hoạt động đầu tiên được xác định hoặc vai trò rõ ràng của nó và sau đó cái tên ban đầu đó vẫn tồn tại, ngay cả khi các hoạt động mới và có lẽ về cơ bản khác nhau xuất hiện từ các nghiên cứu sau này. Đó là sức mạnh đặc biệt của quá trình tiến hóa khi thực hiện một hoạt động nhất định và sau đó sử dụng nó nhiều lần để theo đuổi các mục tiêu dường như khác biệt trong một cơ thể trưởng thành hoặc trưởng thành. Nói chung, yếu tố tăng trưởng chuyển đổi β (TGF-β) có liên quan chặt chẽ đến nhiều quá trình biệt hóa và ức chế tăng trưởng, trong quá trình apoptosis và lắng đọng ma trận ngoại bào. Ban đầu được xác định trong vai trò gây ung thư, TGF-β hiện liên quan đến một số rối loạn mạch máu và thấp khớp, có lẽ đáng chú ý nhất là xơ cứng bì. TGF-β đã được xác định là một ảnh hưởng mạnh mẽ đến quá trình hình thành mạch máu, lành vết thương, viêm khớp, phát triển và di căn khối u, và tất nhiên là điều hòa miễn dịch. Vì vậy, \"tên gọi có ý nghĩa gì?\" Một bông hồng với bất kỳ tên nào khác cũng sẽ có mùi thơm như vậy và vẫn hoạt động miễn dịch, ngay cả khi cái tên đó là \"gây hiểu lầm\"."} +{"idx": 1990060, "en": "We present an unusual case of a 26-year-old man with muscular polyarteritis nodosa (PAN) with severe calf pain and gait disturbance. Magnetic resonance imaging of the lower limbs demonstrated highly increased signal intensity in both soleus muscles and the lateral head of the left gastrocnemius muscle. Biopsies of the soleus muscle showed acute necrotizing arteritis. The calf pain and limited range of motion of ankle dorsiflexion subsided from day 1 on administration of oral corticosteroid at high dosage and were completely resolved by 4 months. After tapering corticosteroid to 10 mg, symptoms recurred. A combined regimen of immunosuppressants was found to maintain symptomatic relief.Muscular PAN should be included in the differential diagnosis of a patient presenting with symptoms of acute or subacute calf pain. Although this muscular PAN was so far been benign, complete remission of the underlying process may be difficult to achieve.", "gemini": "Chúng tôi trình bày một trường hợp bất thường của một người đàn ông 26 tuổi mắc bệnh đa động mạch dạng nốt (PAN) có đau bắp chân dữ dội và rối loạn dáng đi. Chụp cộng hưởng từ các chi dưới cho thấy cường độ tín hiệu tăng cao ở cả hai cơ soleus và phần đầu bên của cơ gastrocnemius trái. Sinh thiết cơ soleus cho thấy viêm động mạch hoại tử cấp tính. Đau bắp chân và phạm vi chuyển động hạn chế của sự gấp mu bàn chân giảm dần từ ngày 1 khi dùng corticosteroid đường uống liều cao và được giải quyết hoàn toàn sau 4 tháng. Sau khi giảm corticosteroid xuống 10 mg, các triệu chứng tái phát. Một chế độ phối hợp thuốc ức chế miễn dịch được phát hiện để duy trì tình trạng giảm triệu chứng. PAN cơ phải được đưa vào chẩn đoán phân biệt của bệnh nhân có các triệu chứng đau bắp chân cấp tính hoặc bán cấp. Mặc dù PAN cơ này cho đến nay vẫn lành tính, nhưng việc thuyên giảm hoàn toàn quá trình cơ bản có thể khó đạt được."} +{"idx": 1990061, "en": "Henoch-Schönlein purpura (HSP) is considered a benign disease of children. We report a severe case of HSP in an adult causing renal failure and gastrointestinal (GI) hemorrhage. Despite aggressive treatment with corticosteroids, cyclophosphamide, and plasmapheresis, the patient developed massive GI bleeding from 2 Dieulafoy lesions and died weeks after bowel resection. Although uncommon, when massive GI hemorrhage occurs, actively bleeding Dieulafoy lesions, although uncommon, should be suspected and evaluated early.", "gemini": "Ban xuất huyết Henoch-Schönlein (HSP) được coi là một bệnh lành tính ở trẻ em. Chúng tôi báo cáo một trường hợp HSP nghiêm trọng ở người lớn gây suy thận và xuất huyết đường tiêu hóa (GI). Mặc dù đã điều trị tích cực bằng corticosteroid, cyclophosphamide và huyết tương, bệnh nhân vẫn bị chảy máu đường tiêu hóa ồ ạt từ 2 tổn thương Dieulafoy và tử vong vài tuần sau khi cắt bỏ ruột. Mặc dù không phổ biến, nhưng khi xảy ra xuất huyết đường tiêu hóa ồ ạt, các tổn thương Dieulafoy chảy máu tích cực, mặc dù không phổ biến, nên được nghi ngờ và đánh giá sớm."} +{"idx": 1990062, "en": "Cytokinins are phytohormones that regulate diverse developmental processes throughout the life of a plant. trans-Zeatin, kinetin, benzyladenine and dihydrozeatin are adenine-type cytokinins that are perceived by the AHK cytokinin receptors. Endogenous cytokinin levels are critical for regulating plant development. To manipulate intracellular cytokinin levels, caged cytokinins were designed on the basis of the crystal structure of the AHK4 cytokinin receptor. The caged cytokinin was photolyzed to release the cytokinin molecule inside the cells and induce cytokinin-responsive gene expression. The uncaging of intracellular caged cytokinins demonstrated that cytokinin-induced root growth inhibition can be manipulated with photo-irradiation. This caged cytokinin system could be a powerful tool for cytokinin biology.", "gemini": "Cytokinin là các phytohormone điều chỉnh các quá trình phát triển đa dạng trong suốt vòng đời của cây. trans-Zeatin, kinetin, benzyladenine và dihydrozeatin là các cytokinin loại adenin được thụ thể cytokinin AHK nhận biết. Nồng độ cytokinin nội sinh rất quan trọng để điều chỉnh sự phát triển của cây. Để thao tác các mức cytokinin trong tế bào, cytokinin lồng được thiết kế trên cơ sở cấu trúc tinh thể của thụ thể cytokinin AHK4. Cytokinin lồng được quang phân để giải phóng phân tử cytokinin bên trong tế bào và gây ra biểu hiện gen đáp ứng với cytokinin. Việc giải phóng cytokinin lồng trong tế bào cho thấy sự ức chế sinh trưởng rễ do cytokinin gây ra có thể được thao tác bằng cách chiếu sáng. Hệ thống cytokinin lồng này có thể là một công cụ mạnh mẽ cho sinh học cytokinin."} +{"idx": 1990063, "en": "Metronidazole thiosalicylate conjugates were synthesized and crystallised in order to discover new molecules having better efficacy than therapeutically administered drug metronidazole, used against Entamoeba histolytica. The three compounds (4-6) showed lower IC(50) values than metronidazole on HM1:IMSS strain of E. histolytica and displayed low cytotoxicity on MCF-7 cell line. In order to get an insight into the mechanisms of action of these compounds, a homology model of E. histolytica thioredoxin reductase (EhTHRase) was constructed and molecular docking was performed into the binding pocket to identify the nature of interactions. The docking studies suggest that the improved inhibitory activity of the newly synthesised metronidazole analogues could be due to involvement of the additional hydrophobic interactions in the binding mode. The result of the present study indicates the molecular fragments that play an essential role in improving the antiamoebic activity.", "gemini": "Các liên hợp metronidazol thiosalicylat được tổng hợp và kết tinh để khám phá các phân tử mới có hiệu quả tốt hơn so với thuốc metronidazol được dùng để điều trị Entamoeba histolytica. Ba hợp chất (4-6) cho thấy giá trị IC(50) thấp hơn metronidazol trên chủng HM1: IMSS của E. histolytica và thể hiện độc tính tế bào thấp trên dòng tế bào MCF-7. Để hiểu sâu hơn về cơ chế hoạt động của các hợp chất này, một mô hình đồng đẳng của enzyme thioredoxin reductase (EhTHRase) của E. histolytica đã được xây dựng và thực hiện ghép nối phân tử vào túi liên kết để xác định bản chất của các tương tác. Các nghiên cứu ghép nối cho thấy rằng hoạt động ức chế được cải thiện của các chất tương tự metronidazol mới tổng hợp có thể là do sự tham gia của các tương tác kỵ nước bổ sung trong chế độ liên kết. Kết quả của nghiên cứu hiện tại chỉ ra các mảnh phân tử đóng vai trò thiết yếu trong việc cải thiện hoạt động diệt amip."} +{"idx": 1990064, "en": "A series of N-(1-methyl-1H-indol-3-yl)methyleneamines and eight new 3,3-diaryl-4-(1-methyl-1H-indol-3-yl)azetidin-2-ones have been synthesized and screened for their antileishmanial activity against Leishmania major. 3,3-Diaryl-4-(1-methyl-1H-indol-3-yl)azetidin-2-ones have been synthesized by the Staudinger's ketene-imine cycloaddition employing two 2-diazo-1,2-diarylethanones as the precursors of diarylketenes. A marked improvement in anti-parasitic activity is observed by transformation of the methyleneamines to azetidin-2-ones in seven out of eight compounds. Two compounds displayed antileishmanial activity comparable to that of the clinically used antileshmanial drug, amphotericine B.", "gemini": "Một loạt các N-(1-methyl-1H-indol-3-yl)methyleneamin và tám loại 3,3-diaryl-4-(1-methyl-1H-indol-3-yl)azetidin-2-on mới đã được tổng hợp và sàng lọc hoạt tính diệt Leishmania của chúng đối với Leishmania major. 3,3-Diaryl-4-(1-methyl-1H-indol-3-yl)azetidin-2-on đã được tổng hợp bằng phản ứng cycloaddition keten-imin của Staudinger sử dụng hai 2-diazo-1,2-diarylethanon làm tiền chất của diarylketen. Hoạt tính diệt ký sinh trùng được cải thiện rõ rệt nhờ chuyển đổi methyleneamin thành azetidin-2-on ở bảy trong số tám hợp chất. Hai hợp chất thể hiện hoạt tính diệt Leishmania tương đương với thuốc diệt Leishmania được sử dụng trong lâm sàng, amphotericine B."} +{"idx": 1990065, "en": "A novel series of N-aryl pyrrolidinonyl oxadiazoles were identified as mGluR5 positive allosteric modulators (PAMs). Optimization of the initial lead compound 6a led to the identification of the 12c (-) enantiomer as a potent compound with acceptable in vitro clearance, CYP, hERG and PK properties. Para substituted N-aryl pyrrolidinonyl oxadiazoles are mGluR5 PAMs while the meta and ortho substituted N-aryl pyrrolidinonyl oxadiazoles are negative allosteric modulators (NAMs). Para fluoro substitution on the N-aryl group and meta chloro or methyl substituents on the aryl oxadiazole moiety are optimal for mGluR5 PAM efficacy. The existence of an exquisitely sensitive 'PAM to NAM switch' within this chemotype making it challenging for simultaneous optimization of potency and drug-like properties.", "gemini": "Một loạt oxadiazol pyrrolidinonyl N-aryl mới được xác định là chất điều biến dị lập dương tính mGluR5 (PAM). Tối ưu hóa hợp chất chính 6a ban đầu đã dẫn đến việc xác định đồng phân quang học (-) 12c là một hợp chất mạnh với độ thanh thải trong ống nghiệm, CYP, hERG và các đặc tính PK có thể chấp nhận được. Oxadiazol pyrrolidinonyl N-aryl thay thế para là PAM mGluR5 trong khi oxadiazol pyrrolidinonyl N-aryl thay thế meta và ortho là chất điều biến dị lập âm tính (NAM). Sự thay thế fluoro para trên nhóm N-aryl và các chất thế clo hoặc methyl meta trên nhóm aryl oxadiazol là tối ưu cho hiệu quả PAM mGluR5. Sự tồn tại của một 'công tắc PAM thành NAM' cực kỳ nhạy trong kiểu gen này khiến việc tối ưu hóa đồng thời hiệu lực và các đặc tính giống thuốc trở nên khó khăn."} +{"idx": 1990066, "en": "A new series of fluorinated pyrazoles, 4a-e, were synthesized in good to excellent overall yields (65-82%) from the corresponding chalcones, 3a-e, by ultrasonic irradiation. The newly synthesized compounds were characterized and screened for their in vitro anti-bacterial, anti-fungal, and anti-tubercular activities against Mycobacterium tuberculosis H(37)Rv.", "gemini": "Một loạt các pyrazol được fluor hóa mới, 4a-e, đã được tổng hợp với tổng năng suất từ tốt đến tuyệt vời (65-82%) từ các chalcone tương ứng, 3a-e, bằng cách chiếu sóng siêu âm. Các hợp chất mới được tổng hợp đã được đặc trưng và sàng lọc hoạt tính kháng khuẩn, kháng nấm và chống lao trong ống nghiệm đối với Mycobacterium tuberculosis H(37)Rv."} +{"idx": 1990067, "en": "Twenty five Imine resveratrol analogues (IRAs) were synthesized, replacing the C=C bond in resveratrol with CN bond, as well as substitution modifications on aromatic rings. Radical scavenging activities against DPPH, along with singlet oxygen quenching capacities were evaluated, and further confirmed using density functional theory calculations (DFT). It was found that IRAs bearing ortho-OH on B ring have better radical scavenging activities against DPPH than resveratrol, these compounds were also found to be effective (1)O(2) quenchers. Theoretical studies on the reaction mechanism of these compounds with (1)O(2) suggest that the 1,3-addition to a double bond with a -OH group with the formation of allylic hydroperoxide is the most probable reaction route.", "gemini": "Hai mươi lăm chất tương tự resveratrol Imine (IRA) đã được tổng hợp, thay thế liên kết C=C trong resveratrol bằng liên kết CN, cũng như các sửa đổi thế trên các vòng thơm. Các hoạt tính dọn gốc tự do đối với DPPH, cùng với khả năng làm tắt oxy đơn phân tử đã được đánh giá và được xác nhận thêm bằng các tính toán lý thuyết hàm mật độ (DFT). Người ta thấy rằng các IRA mang ortho-OH trên vòng B có hoạt tính dọn gốc tự do đối với DPPH tốt hơn resveratrol, những hợp chất này cũng được phát hiện là chất làm tắt (1)O(2) hiệu quả. Các nghiên cứu lý thuyết về cơ chế phản ứng của các hợp chất này với (1)O(2) cho thấy rằng phản ứng cộng 1,3 vào liên kết đôi với nhóm -OH với sự hình thành hydroperoxide allylic là con đường phản ứng có khả năng xảy ra nhất."} +{"idx": 1990068, "en": "Soluble oligomers of human islet amyloid polypeptide (h-IAPP) are believed to be the pathogenic species for type 2 diabetes mellitus. In search of the peptide-cleavage agent cleaving oligomers of h-IAPP with low affinity for polymeric aggregates of h-IAPP, a chemical library was constructed by using the Ugi condensation. From the library, a Co(III) complex was discovered to cleave soluble oligomers of h-IAPP in the presence of polymeric aggregates of h-IAPP without being captured by the aggregates considerably. The peptide-cleavage agent inhibited apoptosis of INS-1 cell by h-IAPP even in the presence of preformed polymeric aggregates of h-IAPP. This suggests that target-selective peptide-cleavage agents may be applied clinically not only to diabetes but also to various other amyloid diseases.", "gemini": "Oligomer hòa tan của polypeptide amyloid tiểu đảo tụy ở người (h-IAPP) được cho là loài gây bệnh đối với bệnh đái tháo đường týp 2. Trong quá trình tìm kiếm tác nhân phân cắt peptide cắt các oligomer của h-IAPP có ái lực thấp đối với các tập hợp polyme của h-IAPP, một thư viện hóa học đã được xây dựng bằng cách sử dụng ngưng tụ Ugi. Từ thư viện, một phức hợp Co(III) đã được phát hiện để cắt các oligomer hòa tan của h-IAPP khi có sự hiện diện của các tập hợp polyme của h-IAPP mà không bị các tập hợp này bắt giữ đáng kể. Tác nhân phân cắt peptide ức chế quá trình apoptosis của tế bào INS-1 bởi h-IAPP ngay cả khi có sự hiện diện của các tập hợp polyme h-IAPP đã được hình thành trước. Điều này cho thấy rằng các tác nhân phân cắt peptide chọn lọc mục tiêu có thể được áp dụng lâm sàng không chỉ đối với bệnh tiểu đường mà còn đối với nhiều bệnh amyloid khác."} +{"idx": 1990069, "en": "Recent findings showing a relation between mutations in the Na(V)1.7 channel in humans and altered pain sensation has contributed to increase the attractiveness of this ion channel as target for development of potential analgesics. Amido chromanes 1 and 2 were identified as blockers of the Na(V)1.7 channel and analogues with modifications of the 5-substituent and the carboxamide part of the molecule were prepared to establish the structure-activity relationship. Compounds 13 and 29 with good overall in vitro and in vivo rat PK profile were identified. Furthermore, 29 showed in vivo efficacy in a nociceptive pain model.", "gemini": "Những phát hiện gần đây cho thấy mối quan hệ giữa các đột biến trong kênh Na(V)1.7 ở người và cảm giác đau thay đổi đã góp phần làm tăng sức hấp dẫn của kênh ion này như một mục tiêu để phát triển các thuốc giảm đau tiềm năng. Các hợp chất Amido chromane 1 và 2 được xác định là chất chẹn kênh Na(V)1.7 và các chất tương tự với sửa đổi nhóm thế 5 và nhóm cacboxamit của phân tử đã được chuẩn bị để thiết lập mối quan hệ cấu trúc-hoạt động. Các hợp chất 13 và 29 có hồ sơ PK ở chuột in vitro và in vivo tổng thể tốt đã được xác định. Hơn nữa, 29 cho thấy hiệu quả in vivo trong mô hình đau do đau."} +{"idx": 1990070, "en": "Androgen receptor (AR) is crucial for transcriptional signaling in prostate cancers. The anti-cancer activity of protein kinase CK2 (formerly called casein kinase 2)-specific small molecule inhibitors have been reported in several cancers including prostate cancers. The orally available CX4945, a potent and selective small molecule inhibitor of CK2, has advanced into human clinical trials and has exhibited strong anti-tumor activity. The inhibition of CK2 leads to a down-regulation of the AR-dependent transcription, but the functional relevance of CX4945 to AR-dependent transcription in AR-positive LNCap cells has not been studied yet. Our observation of inhibitory effects of CX4945 on the expression or phosphorylation levels of CK2α, Akt and anti-apoptotic molecules including IAP family members agreed with a previous study showing the effect of CK2 inhibition in cancer cells. This study also provides novel information on the impact of CX4945 in the inhibition of AR-dependent transcriptional activation in LNCap cells via its down-regulation. Pharmacologic inhibition experiment revealed that CX4945 could exhibit its anti-cancer activity in LNCap cells via the independent inhibitions of AR and Akt-survivin signalings.", "gemini": " thụ thể androgen (AR) rất quan trọng đối với tín hiệu phiên mã trong ung thư tuyến tiền liệt. Hoạt động chống ung thư của các chất ức chế phân tử nhỏ đặc hiệu của protein kinase CK2 (trước đây gọi là casein kinase 2) đã được báo cáo trong một số loại ung thư bao gồm cả ung thư tuyến tiền liệt. CX4945 có sẵn qua đường uống, một chất ức chế phân tử nhỏ mạnh và chọn lọc của CK2, đã tiến triển vào các thử nghiệm lâm sàng trên người và đã thể hiện hoạt động chống khối u mạnh. Sự ức chế CK2 dẫn đến sự điều chỉnh xuống của phiên mã phụ thuộc AR, nhưng sự liên quan về mặt chức năng của CX4945 với phiên mã phụ thuộc AR trong các tế bào LNCap dương tính với AR vẫn chưa được nghiên cứu. Quan sát của chúng tôi về tác dụng ức chế của CX4945 đối với mức độ biểu hiện hoặc phosphoryl hóa của CK2α, Akt và các phân tử chống apoptosis bao gồm các thành viên của họ IAP phù hợp với một nghiên cứu trước đó cho thấy tác dụng của ức chế CK2 trong các tế bào ung thư. Nghiên cứu này cũng cung cấp thông tin mới về tác động của CX4945 trong việc ức chế hoạt hóa phiên mã phụ thuộc AR trong các tế bào LNCap thông qua sự điều chỉnh xuống của nó. Thí nghiệm ức chế dược lý cho thấy CX4945 có thể thể hiện hoạt động chống ung thư của nó trong các tế bào LNCap thông qua các ức chế độc lập của các tín hiệu AR và Akt-survivin."} +{"idx": 1990071, "en": "Novel simplified side chains of the potent RNA polymerase inhibitor etnangien were designed, synthesized and evaluated for antibacterial activity against Gram-positive bacteria and one Gram-negative bacterium.", "gemini": "Các chuỗi bên đơn giản mới của chất ức chế RNA polymerase mạnh etnangien đã được thiết kế, tổng hợp và đánh giá hoạt tính kháng khuẩn đối với vi khuẩn Gram dương và một vi khuẩn Gram âm."} +{"idx": 1990072, "en": "Necroptosis is a regulated caspase-independent cell death pathway with morphological features resembling passive non-regulated necrosis. Several diverse structure classes of necroptosis inhibitors have been reported to date, including a series of 3,3a,4,5-tetrahydro-2H-benz[g]indazoles (referred to as the Nec-3 series) displaying potent activity in cellular assays. However, evaluation of the tricyclic necroptosis inhibitor's stability in mouse liver microsomes indicated that they were rapidly degraded. A structure-activity relationship (SAR) study of this compound series revealed that increased liver microsomal stability could be accomplished by modification of the pendent phenyl ring and by introduction of a hydrophilic substituent (i.e., α-hydroxyl) to the acetamide at the 2-position of the tricyclic ring without significantly compromising necroptosis inhibitory activity. Further increases in microsomal stability could be achieved by utilizing the 5,5-dioxo-3-phenyl-2,3,3a,4-tetrahydro-[1]benzothiopyrano[4,3-c]pyrazoles. However, in this case necroptosis inhibitory activity was not maintained. Overall, these results provide a strategy for generating potent and metabolically stable tricyclic necrostatin analogs (e.g., 33, LDN-193191) potentially suitable for in vivo studies.", "gemini": "Hoại tử tế bào theo chương trình là một con đường chết tế bào không phụ thuộc caspase được điều hòa với các đặc điểm hình thái giống như hoại tử thụ động không được điều hòa. Cho đến nay, một số lớp cấu trúc khác nhau của chất ức chế hoại tử tế bào theo chương trình đã được báo cáo, bao gồm một loạt các 3,3a,4,5-tetrahydro-2H-benz[g]indazol (được gọi là loạt Nec-3) thể hiện hoạt tính mạnh trong các xét nghiệm tế bào. Tuy nhiên, đánh giá độ ổn định của chất ức chế hoại tử tế bào theo chương trình ba vòng trong các vi thể gan chuột cho thấy chúng bị phân hủy nhanh chóng. Một nghiên cứu về mối quan hệ cấu trúc-hoạt động (SAR) của loạt hợp chất này cho thấy có thể đạt được độ ổn định của vi thể gan tăng lên bằng cách sửa đổi vòng phenyl lơ lửng và bằng cách đưa vào một chất thế ưa nước (tức là α-hydroxyl) vào acetamid ở vị trí thứ 2 của vòng ba vòng mà không làm giảm đáng kể hoạt tính ức chế hoại tử tế bào theo chương trình. Có thể đạt được độ ổn định của vi thể cao hơn nữa bằng cách sử dụng các 5,5-dioxo-3-phenyl-2,3,3a,4-tetrahydro-[1]benzothiopyrano[4,3-c]pyrazol. Tuy nhiên, trong trường hợp này, hoạt tính ức chế hoại tử tế bào theo chương trình không được duy trì. Nhìn chung, những kết quả này cung cấp một chiến lược để tạo ra các chất tương tự necrostatin ba vòng mạnh và ổn định về mặt chuyển hóa (ví dụ: 33, LDN-193191) có khả năng phù hợp cho các nghiên cứu in vivo."} +{"idx": 1990073, "en": "This letter describes the synthesis and biological evaluation of a novel series of radioiodinated oxindole (OI) derivatives for detecting neurofibrillary tangles (NFTs) in the brains of patients with Alzheimer's disease (AD). In binding experiments in vitro, 2-oxindole (2-OI) and 3-oxindole (3-OI) derivatives showed affinity for tau aggregates. The 3-OI derivative 14 showed the highest affinity of these derivatives. In biodistribution experiments using normal mice, the OI derivatives displayed good uptake (2.4-2.5%ID/g at 2 min) and clearance from the brain with time (0.6-1.4%ID/g at 30 min). In fluorescence staining experiments using AD brain sections, 14 clearly stained NFTs. 3-OI may serve as a new molecular scaffold for developing novel NFT imaging agents.", "gemini": "Thư này mô tả quá trình tổng hợp và đánh giá sinh học của một loạt các dẫn xuất oxindole (OI) phóng xạ mới để phát hiện các đám rối sợi thần kinh (NFT) trong não của bệnh nhân mắc bệnh Alzheimer (AD). Trong các thí nghiệm liên kết in vitro, các dẫn xuất 2-oxindole (2-OI) và 3-oxindole (3-OI) cho thấy ái lực đối với các tập hợp tau. Dẫn xuất 3-OI 14 cho thấy ái lực cao nhất trong số các dẫn xuất này. Trong các thí nghiệm phân bố sinh học sử dụng chuột bình thường, các dẫn xuất OI cho thấy sự hấp thu tốt (2,4-2,5%ID/g ở phút thứ 2) và đào thải khỏi não theo thời gian (0,6-1,4%ID/g ở phút thứ 30). Trong các thí nghiệm nhuộm huỳnh quang sử dụng các lát não AD, 14 nhuộm rõ ràng các NFT. 3-OI có thể đóng vai trò là một khung phân tử mới để phát triển các tác nhân tạo ảnh NFT mới."} +{"idx": 1990074, "en": "In the present communication we prepared a series of six 4-hydroxycoumarin derivatives, isosters of quercetin, recognized as an antioxidant natural compound, with the aim of evaluating the antitrypanosomal activity against Trypanosoma cruzi, the parasite responsible for Chagas disease, and the antioxidant properties. We have used the 4-hydroxycoumarin moiety (compound 1) as the molecular template for the synthesis of compounds 2-7. These derivates have shown moderate trypanocidal activity. However they have been proved to be good antioxidants. In particular compound 7 is the most active antioxidant and it is, therefore, a potential candidate for a successful employment in conditions characterized by free radicals overproduction.", "gemini": "Trong thông báo hiện tại, chúng tôi đã điều chế một loạt sáu dẫn xuất 4-hydroxycoumarin, các đồng phân cấu tạo của quercetin, được công nhận là một hợp chất tự nhiên chống oxy hóa, với mục đích đánh giá hoạt tính chống trypanosoma đối với Trypanosoma cruzi, ký sinh trùng gây bệnh Chagas và các đặc tính chống oxy hóa. Chúng tôi đã sử dụng nhóm 4-hydroxycoumarin (hợp chất 1) làm khuôn mẫu phân tử để tổng hợp các hợp chất 2-7. Các dẫn xuất này đã cho thấy hoạt tính diệt trypanocidal vừa phải. Tuy nhiên, chúng đã được chứng minh là chất chống oxy hóa tốt. Đặc biệt hợp chất 7 là chất chống oxy hóa hoạt động mạnh nhất và do đó, nó là một ứng cử viên tiềm năng để sử dụng thành công trong các tình trạng đặc trưng bởi tình trạng sản xuất quá mức các gốc tự do."} +{"idx": 1990075, "en": "A series of Pictet-Spengler condensation derivatives (tetrahydro-β-carbolines) was designed, synthesized and evaluated for lethality against a panel of seven cancer cell lines. Seven compounds (2a, 13, 20, 21, 27, 29 and 34) showed lethality in at least five cell lines. Among these, compound 27 showed a unique selectivity towards oncogenic-RAS expressing BJ-TERT/LT/ST/RAS(V12) tumor cells, compared to non-transformed BJ-TERT cells. Further investigation revealed that 27 induces cell death without activation of caspases. This represents a useful new probe of non-apoptotic cell death and oncogenic-RAS synthetic lethality.", "gemini": "Một loạt các dẫn xuất ngưng tụ Pictet-Spengler (tetrahydro-β-carbolines) được thiết kế, tổng hợp và đánh giá về khả năng gây chết đối với một nhóm bảy dòng tế bào ung thư. Bảy hợp chất (2a, 13, 20, 21, 27, 29 và 34) cho thấy khả năng gây chết ở ít nhất năm dòng tế bào. Trong số đó, hợp chất 27 cho thấy tính chọn lọc độc đáo đối với tế bào khối u BJ-TERT/LT/ST/RAS(V12) biểu hiện oncogenic-RAS, so với tế bào BJ-TERT không chuyển đổi. Các nghiên cứu sâu hơn cho thấy 27 gây chết tế bào mà không kích hoạt caspase. Điều này đại diện cho một đầu dò mới hữu ích về chết tế bào không theo chương trình và gây chết tổng hợp oncogenic-RAS."} +{"idx": 1990076, "en": "Phosphoinositide 3-kinase (PI3K) is an important target in oncology due to the deregulation of the PI3K/Akt signaling pathway in a wide variety of tumors. A series of 4-amino-6-methyl-1,3,5-triazine sulfonamides were synthesized and evaluated as inhibitors of PI3K. The synthesis, in vitro biological activities, pharmacokinetic and in vivo pharmacodynamic profiling of these compounds are described. The most promising compound from this investigation (compound 3j) was found to be a pan class I PI3K inhibitor with a moderate (>10-fold) selectivity over the mammalian target of rapamycin (mTOR) in the enzyme assay. In a U87 MG cellular assay measuring phosphorylation of Akt, compound 3j displayed low double digit nanomolar IC(50) and exhibited good oral bioavailability in rats (F(oral)=63%). Compound 3j also showed a dose dependent reduction in the phosphorylation of Akt in a U87 tumor pharmacodynamic model with a plasma EC(50)=193 nM (91 ng/mL).", "gemini": "Phosphoinositide 3-kinase (PI3K) là một mục tiêu quan trọng trong ung thư do sự mất điều hòa của con đường truyền tín hiệu PI3K/Akt trong nhiều loại khối u. Một loạt các sulfonamid 4-amino-6-methyl-1,3,5-triazine đã được tổng hợp và đánh giá như các chất ức chế PI3K. Bài báo mô tả quá trình tổng hợp, hoạt động sinh học trong ống nghiệm, dược động học và lập hồ sơ dược lực học trong cơ thể của các hợp chất này. Hợp chất triển vọng nhất từ cuộc điều tra này (hợp chất 3j) được phát hiện là chất ức chế PI3K lớp I chảo với tính chọn lọc vừa phải (>10 lần) đối với mục tiêu của động vật có vú đối với rapamycin (mTOR) trong xét nghiệm enzyme. Trong xét nghiệm tế bào U87 MG để đo sự phosphoryl hóa của Akt, hợp chất 3j cho thấy IC(50) nanomol hai chữ số thấp và thể hiện khả dụng sinh học đường uống tốt ở chuột (F(uống)=63%). Hợp chất 3j cũng cho thấy sự giảm phụ thuộc liều lượng trong quá trình phosphoryl hóa Akt trong mô hình dược lực học khối u U87 với EC(50) huyết tương=193 nM (91 ng/mL)."} +{"idx": 1990077, "en": "BACKGROUND/AIMS Disorders of sex development (DSD) are congenital conditions in which chromosomal, gonadal, or anatomic sex development is atypical. Optimal management is patient- and family-centered and delivered by interdisciplinary teams. The present pilot study elicits concerns held by important stakeholders on issues affecting young patients with DSD and their families. METHODS Content from focus groups with expert clinicians (pediatric urologists (n = 7), pediatric endocrinologists (n = 10), mental health professionals (n = 4), DSD patient advocates (n = 4), and interviews with parents of DSD-affected children (newborn to 6 years; n = 11) was coded and content-analyzed to identify health-related quality of life issues. RESULTS Key stressors varied across stakeholder groups. In general, family-centered issues were noted more than child-centered. In the child-centered domain, providers worried more about physical functioning; family and advocates emphasized gender concerns and body image. In the family-centered domain, parental concerns about medication management outweighed those of providers. Advocates reported more stressors regarding communication/information than other stakeholders. CONCLUSION Variability exists across stakeholder groups in the key concerns affecting young children/families with DSD. Interdisciplinary DSD healthcare team development should account for varying perspectives when counseling families and planning treatment.", "gemini": "BỐI CẢNH/MỤC ĐÍCH Rối loạn phát triển giới tính (DSD) là tình trạng bẩm sinh trong đó sự phát triển giới tính về nhiễm sắc thể, tuyến sinh dục hoặc giải phẫu là không điển hình. Quản lý tối ưu là lấy bệnh nhân và gia đình làm trung tâm và được thực hiện bởi các nhóm liên ngành. Nghiên cứu thí điểm hiện nay nêu ra những lo ngại của các bên liên quan quan trọng về các vấn đề ảnh hưởng đến bệnh nhân trẻ mắc DSD và gia đình của họ. PHƯƠNG PHÁP Nội dung từ các nhóm tập trung với các bác sĩ lâm sàng chuyên gia (bác sĩ tiết niệu nhi khoa (n = 7), bác sĩ nội tiết nhi khoa (n = 10), chuyên gia sức khỏe tâm thần (n = 4), người ủng hộ bệnh nhân DSD (n = 4) và phỏng vấn với cha mẹ của trẻ em mắc DSD (trẻ sơ sinh đến 6 tuổi; n = 11) đã được mã hóa và phân tích nội dung để xác định các vấn đề về chất lượng cuộc sống liên quan đến sức khỏe. KẾT QUẢ Các tác nhân gây căng thẳng chính khác nhau giữa các nhóm bên liên quan. Nhìn chung, các vấn đề lấy gia đình làm trung tâm được ghi nhận nhiều hơn các vấn đề lấy trẻ em làm trung tâm. Trong phạm vi lấy trẻ em làm trung tâm, các nhà cung cấp lo lắng nhiều hơn về chức năng thể chất; gia đình và những người ủng hộ nhấn mạnh đến các mối quan tâm về giới tính và hình ảnh cơ thể. Trong phạm vi lấy gia đình làm trung tâm, mối quan tâm của cha mẹ về việc quản lý thuốc lớn hơn mối quan tâm của các nhà cung cấp. Những người ủng hộ báo cáo nhiều tác nhân gây căng thẳng liên quan đến giao tiếp/thông tin hơn các bên liên quan khác. KẾT LUẬN Sự khác biệt tồn tại giữa các nhóm bên liên quan về những mối quan tâm chính ảnh hưởng đến trẻ nhỏ/gia đình mắc DSD. Sự phát triển của nhóm chăm sóc sức khỏe DSD liên ngành nên tính đến các quan điểm khác nhau khi tư vấn cho gia đình và lập kế hoạch điều trị."} +{"idx": 1990078, "en": "Gender-based violence is a key determinant of HIV infection among women in South Africa as elsewhere. However, research has not examined potential mediating processes to explain the link between experiencing abuse and engaging in HIV sexual risk behavior. Previous studies suggest that alcohol use and mental health problems may explain how gender-based violence predicts sexual risk. In a prospective study, we examined whether lifetime history of gender-based violence indirectly affects future sexual risk behavior through alcohol use, depression and post-traumatic stress disorder (PTSD) in a high-risk socio-environmental context. We recruited a cohort of 560 women from alcohol drinking venues in a Cape Town, South African township. Participants completed computerized interviews at baseline and 4 months later. We tested prospective mediating associations between gender-based violence, alcohol use, depression, PTSD, and sexual risk behavior. There was a significant indirect effect of gender-based violence on sexual risk behavior through alcohol use, but not mental health problems. Women who were physically and sexually abused drank more, which in turn predicted more unprotected sex. We did not find a mediated relationship between alcohol use and sexual risk behavior through the experience of recent abuse or mental health problems. Alcohol use explains the link between gender-based violence and sexual risk behavior among women attending drinking venues in Cape Town, South Africa. Efforts to reduce HIV risk in South Africa by addressing gender-based violence must also address alcohol use.", "gemini": "Bạo lực dựa trên giới là một yếu tố quyết định chính đối với nhiễm HIV ở phụ nữ ở Nam Phi cũng như các nơi khác. Tuy nhiên, nghiên cứu chưa xem xét các quá trình trung gian tiềm ẩn để giải thích mối liên hệ giữa việc bị lạm dụng và tham gia vào hành vi tình dục có nguy cơ lây nhiễm HIV. Các nghiên cứu trước đây cho thấy rằng việc sử dụng rượu và các vấn đề về sức khỏe tâm thần có thể giải thích cách bạo lực dựa trên giới dự đoán nguy cơ tình dục. Trong một nghiên cứu triển vọng, chúng tôi đã xem xét liệu tiền sử bạo lực dựa trên giới có ảnh hưởng gián tiếp đến hành vi tình dục có nguy cơ trong tương lai thông qua việc sử dụng rượu, trầm cảm và rối loạn căng thẳng sau chấn thương (PTSD) trong bối cảnh xã hội - môi trường có nguy cơ cao hay không. Chúng tôi đã tuyển dụng một nhóm gồm 560 phụ nữ từ các địa điểm uống rượu tại một thị trấn Cape Town, Nam Phi. Những người tham gia đã hoàn thành các cuộc phỏng vấn bằng máy tính khi bắt đầu và 4 tháng sau đó. Chúng tôi đã kiểm tra các mối liên hệ trung gian triển vọng giữa bạo lực dựa trên giới, sử dụng rượu, trầm cảm, PTSD và hành vi tình dục có nguy cơ. Có một tác động gián tiếp đáng kể của bạo lực dựa trên giới đối với hành vi tình dục có nguy cơ thông qua việc sử dụng rượu, nhưng không phải các vấn đề về sức khỏe tâm thần. Những phụ nữ bị bạo hành về thể chất và tình dục uống nhiều hơn, và điều này dự đoán nhiều hơn về quan hệ tình dục không được bảo vệ. Chúng tôi không tìm thấy mối quan hệ trung gian giữa việc sử dụng rượu và hành vi tình dục có nguy cơ thông qua trải nghiệm bị lạm dụng gần đây hoặc các vấn đề về sức khỏe tâm thần. Việc sử dụng rượu giải thích mối liên hệ giữa bạo lực dựa trên giới và hành vi tình dục có nguy cơ ở những phụ nữ đến các địa điểm uống rượu ở Cape Town, Nam Phi. Những nỗ lực nhằm giảm nguy cơ HIV ở Nam Phi bằng cách giải quyết vấn đề bạo lực dựa trên giới cũng phải giải quyết vấn đề sử dụng rượu."} +{"idx": 1990079, "en": "Concerns regarding sleep disorders in Hmong immigrants in the US emerged when an astonishingly high mortality rate of Sudden Unexplained Nocturnal Death Syndrome (SUNDS) was documented in Hmong men. Stress, genetics, and cardiac abnormalities interacting with disordered sleep were hypothesized as contributing factors to SUNDS. Most recently, sleep apnea has been implicated in nighttime deaths of Brugada Syndrome. This syndrome is thought to comprise a spectrum of sudden cardiac death disorders, including SUNDS. However, little research since has placed SUNDS in its context of Hmong cultural beliefs, health, or the prevalence of other sleep disorders. Because the epidemiology of sleep disorders and terrifying nighttime experiences in Hmong is poorly documented, we investigated the prevalence and correlates of sleep apnea, rapid eye movement (REM) sleep stage related disorders, and insomnia in 3 population-based samples (collected from 1996 to 2001) comprising 747 Hmong immigrants in Wisconsin. Participants were questioned on sleep problems, cultural beliefs, health, and other factors. A random subsample (n = 37) underwent in-home polysomnography to investigate sleep apnea prevalence. Self-report and laboratory findings were compared with similarly collected data from the Wisconsin Sleep Cohort (WSC) study (n = 1170), a population-based longitudinal study of sleep. The results inform a unique Hmong sleep disorder profile of a high prevalence of sleep apnea, sleep paralysis, and other REM-related sleep abnormalities as well the interaction of culturally related nighttime stressors with these sleep problems. For example, experiences of dab tsog (frightening night spirit pressing on chest) was prevalent and related to sleep apnea indicators, sleep paralysis, nightmares, hypnogogic hallucinations, and insomnia. Understanding the role of sleep disorders and the cultural mechanisms that may trigger or condition response to them could ultimately provide a basis for screening and intervention to reduce the adverse health and emotional consequences of these conditions in Hmong.", "gemini": "Mối quan tâm liên quan đến các rối loạn giấc ngủ ở những người nhập cư Hmong ở Hoa Kỳ xuất hiện khi tỷ lệ tử vong cao đáng kinh ngạc do Hội chứng tử vong đột ngột không rõ nguyên nhân về đêm (SUNDS) được ghi nhận ở nam giới Hmong. Căng thẳng, di truyền và bất thường về tim tương tác với giấc ngủ rối loạn được đưa ra giả thuyết là những yếu tố góp phần gây ra SUNDS. Gần đây nhất, chứng ngưng thở khi ngủ đã liên quan đến tử vong về đêm của Hội chứng Brugada. Hội chứng này được cho là bao gồm một loạt các rối loạn tử vong tim đột ngột, bao gồm cả SUNDS. Tuy nhiên, ít nghiên cứu nào kể từ đó đặt SUNDS vào bối cảnh về niềm tin văn hóa, sức khỏe hoặc tỷ lệ mắc các rối loạn giấc ngủ khác của người Hmong. Do dịch tễ học của các rối loạn giấc ngủ và những trải nghiệm kinh hoàng về đêm ở người Hmong chưa được ghi chép đầy đủ, chúng tôi đã điều tra tỷ lệ mắc và mối tương quan của chứng ngưng thở khi ngủ, các rối loạn liên quan đến giai đoạn ngủ chuyển động mắt nhanh (REM) và chứng mất ngủ trong 3 mẫu dựa trên dân số (thu thập từ năm 1996 đến năm 2001) bao gồm 747 người nhập cư Hmong ở Wisconsin. Những người tham gia được hỏi về các vấn đề về giấc ngủ, niềm tin văn hóa, sức khỏe và các yếu tố khác. Một nhóm mẫu ngẫu nhiên (n = 37) đã trải qua đa ký giấc ngủ tại nhà để điều tra tỷ lệ mắc chứng ngưng thở khi ngủ. Các phát hiện tự báo cáo và trong phòng thí nghiệm đã được so sánh với dữ liệu được thu thập tương tự từ nghiên cứu Wisconsin Sleep Cohort (WSC) (n = 1170), một nghiên cứu theo chiều dọc dựa trên dân số về giấc ngủ. Các kết quả cho biết một hồ sơ rối loạn giấc ngủ độc đáo của người Hmong có tỷ lệ mắc chứng ngưng thở khi ngủ, chứng liệt ngủ và các bất thường về giấc ngủ liên quan đến REM khác cao cũng như sự tương tác của các yếu tố gây căng thẳng về đêm có liên quan đến văn hóa với những vấn đề về giấc ngủ này. Ví dụ, trải nghiệm dab tsog (linh hồn đêm đáng sợ đè lên ngực) rất phổ biến và liên quan đến các chỉ số ngưng thở khi ngủ, liệt ngủ, ác mộng, ảo giác thôi miên và mất ngủ. Hiểu được vai trò của các rối loạn giấc ngủ và các cơ chế văn hóa có thể kích hoạt hoặc điều kiện phản ứng với chúng cuối cùng có thể cung cấp cơ sở để sàng lọc và can thiệp nhằm giảm hậu quả bất lợi về sức khỏe và cảm xúc của những tình trạng này ở người Hmong."} +{"idx": 1990080, "en": "BACKGROUND/AIMS Ketamine has been reported to exert anti-inflammatory effects on astrocytes stimulated by lipopolysaccharide (LPS) in vitro and in vivo. However, the mechanism has not been elicited clearly. The aim of this study was to investigate the effects of ketamine on TLR4 expression and NF-ĸB-p65 phosphorylation, as well as the production of proinflammatory cytokines in LPS challenged astrocytes. METHODS Astrocytes were stimulated with LPS (1µg/ ml) in the absence and presence of various concentrations of ketamine (10, 100, 1000µM). The concentrations of IL-1β, IL-6 and TNF-α were measured by ELISA, the expression of glial fibrillary acidic protein (GFAP) in astrocytes was detected by immunofluorescence staining, the level of phosphorylated NF-ĸB p65 and the expression of TLR4 were detected by western blotting. RESULTS LPS increased TLR4 expression and the phosphorylation of NF-ĸB p65 subunit as well as GFAP expression and the production of IL-1β, IL-6 and TNF-α in cultured astrocytes. Ketamine (100 and 1000 µM) reduced the expression of GFAP and the production of these proinflammatory cytokines, inhibited the expression of TLR4 and attenuated the phosphorylation of NF-ĸB p65 in astrocytes challenged by LPS. CONCLUSION The inhibitory effects of ketamine on LPS-induced astrocytes activation and inflammation response may be mediated by suppressing NF-ĸB activation through reducing the expression of TLR4.", "gemini": "BỐI CẢNH/MỤC ĐÍCH Ketamine đã được báo cáo là có tác dụng chống viêm trên tế bào hình sao được kích thích bởi lipopolysaccharide (LPS) trong ống nghiệm và trong cơ thể sống. Tuy nhiên, cơ chế này vẫn chưa được làm rõ. Mục đích của nghiên cứu này là để điều tra các tác động của ketamine lên biểu hiện TLR4 và phosphoryl hóa NF-ĸB-p65, cũng như sự sản xuất các cytokine tiền viêm trong các tế bào hình sao bị LPS tấn công. PHƯƠNG PHÁP Các tế bào hình sao được kích thích bằng LPS (1µg/ml) khi không có và có sự hiện diện của nhiều nồng độ ketamine khác nhau (10, 100, 1000µM). Nồng độ IL-1β, IL-6 và TNF-α được đo bằng ELISA, biểu hiện của protein axit sợi gai (GFAP) trong tế bào hình sao được phát hiện bằng phương pháp nhuộm miễn dịch huỳnh quang, mức độ phosphoryl hóa NF-ĸB p65 và biểu hiện của TLR4 được phát hiện bằng phương pháp blotting. KẾT QUẢ LPS làm tăng biểu hiện TLR4 và phosphoryl hóa tiểu đơn vị NF-ĸB p65 cũng như biểu hiện GFAP và sản xuất IL-1β, IL-6 và TNF-α trong các tế bào hình sao được nuôi cấy. Ketamine (100 và 1000 µM) làm giảm biểu hiện GFAP và sản xuất các cytokine tiền viêm này, ức chế biểu hiện TLR4 và làm giảm phosphoryl hóa NF-ĸB p65 trong các tế bào hình sao bị LPS tấn công. KẾT LUẬN Các tác dụng ức chế của ketamine đối với sự hoạt hóa tế bào hình sao do LPS gây ra và đáp ứng viêm có thể được trung gian thông qua việc ức chế hoạt hóa NF-ĸB bằng cách giảm biểu hiện TLR4."} +{"idx": 1990081, "en": "OBJECTIVE The purpose of this study was to investigate the expression of metabolism-related proteins such as Glut-1 and carbonic anhydrase IX (CAIX) according to breast cancer molecular subtype. METHODS We generated a tissue microarray of 276 breast cancer patients and performed immunohistochemical staining for known metabolism-related proteins, which were evaluated according to the molecular subtype. RESULTS The expression of IGF-1, MIF, and HIF-1α was correlated with the HER-2 type (p < 0.05). Glut-1 overexpression and CAIX expression were associated with TNBC type, especially with basal-like type, high histologic grade, estrogen receptor negativity, and progesterone receptor negativity (p < 0.05). The expression of Glut-1 and CAIX was correlated with statistical significance (p < 0.001). CONCLUSION We identified different patterns of expression of metabolism-related proteins according to the molecular subtypes of breast cancer defined by surrogate immunohistochemistry. Increased expression of HIF-1α, IGF-1, and MIF was noted in HER-2 type breast cancer and increased expression of Glut-1 and CAIX was noted in TNBC type breast cancer, especially in the basal-like subtype, which exhibited a glycolytic and acid-resistant tumor phenotype.", "gemini": "MỤC TIÊU Mục đích của nghiên cứu này là tìm hiểu biểu hiện của các protein liên quan đến chuyển hóa như Glut-1 và carbonic anhydrase IX (CAIX) theo phân nhóm phân tử ung thư vú. PHƯƠNG PHÁP Chúng tôi đã tạo ra một mảng mô của 276 bệnh nhân ung thư vú và tiến hành nhuộm miễn dịch hóa mô đối với các protein liên quan đến chuyển hóa đã biết, được đánh giá theo phân nhóm phân tử. KẾT QUẢ Biểu hiện của IGF-1, MIF và HIF-1α có tương quan với loại HER-2 (p < 0,05). Sự biểu hiện quá mức của Glut-1 và biểu hiện của CAIX có liên quan đến loại TNBC, đặc biệt là loại giống cơ bản, độ biệt hóa mô học cao, thụ thể estrogen âm tính và thụ thể progesterone âm tính (p < 0,05). Biểu hiện của Glut-1 và CAIX có tương quan với ý nghĩa thống kê (p < 0,001). KẾT LUẬN Chúng tôi đã xác định các kiểu biểu hiện khác nhau của các protein liên quan đến chuyển hóa theo các phân nhóm phân tử của ung thư vú được xác định bằng phương pháp miễn dịch hóa mô học thay thế. Sự biểu hiện tăng lên của HIF-1α, IGF-1 và MIF được ghi nhận ở ung thư vú loại HER-2 và sự biểu hiện tăng lên của Glut-1 và CAIX được ghi nhận ở ung thư vú loại TNBC, đặc biệt là ở phân nhóm giống cơ bản, biểu hiện kiểu hình khối u ưa glycolytic và kháng axit."} +{"idx": 1990082, "en": "In general, Japanese and Caucasians differ in their response to alcohol. To investigate these differences the alcohol clamping method can be used. This strictly controlled infusion regimen provides a reliable tool to study contrasts in central nervous system (CNS) effects and/or alcohol disposition. In this study, twelve Japanese and twelve Caucasian healthy volunteers received two concentrations of intravenous alcohol or placebo using the alcohol clamp. Infusion rates during the steady state phase were used to compare alcohol clearance between the subgroups. Central nervous system (CNS) effects were frequently measured throughout the clamp. On average, significantly lower amounts of alcohol were needed to maintain similar stable concentrations in the Japanese group. However, these differences disappeared when values were corrected for lean body mass. The most pronounced pharmacodynamic differences between the groups were observed on body sway and on the visual analogue scale for subjective alcohol effects, mainly at the highest dose level. The alcohol clamp seems a useful method to compare differences in alcohol metabolism between groups. Some CNS effects of alcohol differed clearly between Japanese and Caucasians, but others did not, even though alcohol levels were stable and similar between the two groups.", "gemini": "Nhìn chung, người Nhật Bản và người da trắng có phản ứng khác nhau với rượu. Để nghiên cứu những khác biệt này, có thể sử dụng phương pháp kẹp rượu. Chế độ truyền được kiểm soát chặt chẽ này cung cấp một công cụ đáng tin cậy để nghiên cứu sự tương phản trong tác dụng của hệ thần kinh trung ương (CNS) và/hoặc sự phân bố của rượu. Trong nghiên cứu này, mười hai người Nhật Bản và mười hai tình nguyện viên khỏe mạnh người da trắng đã nhận được hai nồng độ rượu tiêm tĩnh mạch hoặc giả dược bằng cách sử dụng kẹp rượu. Tốc độ truyền trong giai đoạn ổn định được sử dụng để so sánh độ thanh thải rượu giữa các nhóm phụ. Các tác dụng của hệ thần kinh trung ương (CNS) thường được đo trong suốt quá trình kẹp. Trung bình, cần một lượng rượu thấp hơn đáng kể để duy trì nồng độ ổn định tương tự ở nhóm người Nhật. Tuy nhiên, những khác biệt này biến mất khi các giá trị được hiệu chỉnh theo khối lượng cơ nạc. Sự khác biệt về dược động học rõ rệt nhất giữa các nhóm được quan sát thấy ở độ lắc lư cơ thể và trên thang tương tự thị giác đối với các tác dụng chủ quan của rượu, chủ yếu ở mức liều cao nhất. Kẹp rượu dường như là một phương pháp hữu ích để so sánh sự khác biệt về chuyển hóa rượu giữa các nhóm. Một số tác dụng của rượu đối với CNS khác nhau rõ ràng giữa người Nhật Bản và người da trắng, nhưng một số khác thì không, mặc dù nồng độ rượu ổn định và tương tự nhau giữa hai nhóm."} +{"idx": 1990083, "en": "Sequestration and diffusion of three polycyclic aromatic hydrocarbons (PAHs) in seven Chinese soils were investigated for up to 200 days in sterile soil microcosms as functions of soil property and aging time. The aging of the PAHs, assessed using a mild extractant that removes primarily the labile fraction, showed a biphasic behavior. The rapid diffusion from labile to nonlabile domains was mainly dependent upon the distribution of meso- and micropore fraction and total organic carbon content. Meanwhile, the slow diffusion was found to decrease with the increase of the content of soil organic carbon, particularly of hard organic carbon (p < 0.01) and the meso- and micropore fraction, as well as with the increasing molecular size of PAHs. This work offers evidence that analyses of organic carbon fractionation and porosity are important to adequately assess the mechanistic basis of sequestration and diffusion of organic contaminants in soils.", "gemini": "Sự cô lập và khuếch tán của ba hydrocarbon thơm đa vòng (PAH) trong bảy loại đất của Trung Quốc đã được nghiên cứu trong tối đa 200 ngày trong các vi mô đất vô trùng như các chức năng của tính chất đất và thời gian lão hóa. Sự lão hóa của PAH, được đánh giá bằng cách sử dụng một chất chiết xuất nhẹ chủ yếu loại bỏ phần không bền, cho thấy hành vi hai pha. Sự khuếch tán nhanh từ miền không bền sang miền không bền chủ yếu phụ thuộc vào sự phân bố của phần trung bình và vi lỗ và hàm lượng carbon hữu cơ toàn phần. Trong khi đó, sự khuếch tán chậm được phát hiện giảm khi hàm lượng carbon hữu cơ trong đất tăng lên, đặc biệt là carbon hữu cơ cứng (p < 0,01) và phần trung bình và vi lỗ, cũng như khi kích thước phân tử của PAH tăng lên. Công trình này đưa ra bằng chứng cho thấy các phân tích về phân đoạn carbon hữu cơ và độ xốp rất quan trọng để đánh giá đầy đủ cơ sở cơ học của sự cô lập và khuếch tán của các chất gây ô nhiễm hữu cơ trong đất."} +{"idx": 1990084, "en": "Sixteen soil samples were collected from the central Tibetan Plateau (CTP). The soil concentrations of polybrominated diphenyl ethers (PBDEs) in CTP were analyzed. The detected 42 congeners were divided into light, intermediate and heavy fractions. In addition to the various minerals, other soil properties were also characterized, including the content of soil organic carbon (SOC) and the particle size distribution. The clay content is positively related to the intermediate fraction of the PBDEs and negatively related to the light and heavy fractions. Similar correlations were observed for SOC and the fine-particle fraction (size < 2 μm). The coefficient of determination (r(2)) associated with a linear regression indicated that the clays were more highly correlated with the fractional pattern of the PBDEs than with the other properties, such as SOC and the fine-particle fraction. The values of r(2) between clays and three fractions of PBDEs are 0.70, 0.69 and 0.58.", "gemini": "Mười sáu mẫu đất đã được thu thập từ Cao nguyên Tây Tạng (CTP). Nồng độ polybrominated diphenyl ete (PBDE) trong đất ở CTP đã được phân tích. 42 đồng phân được phát hiện được chia thành các phân đoạn nhẹ, trung bình và nặng. Ngoài các khoáng chất khác nhau, các tính chất khác của đất cũng được đặc trưng, bao gồm hàm lượng cacbon hữu cơ trong đất (SOC) và phân bố kích thước hạt. Hàm lượng đất sét có mối quan hệ thuận với phân đoạn trung gian của PBDE và có mối quan hệ nghịch với phân đoạn nhẹ và nặng. Các mối tương quan tương tự đã được quan sát thấy đối với SOC và phân đoạn hạt mịn (kích thước < 2 μm). Hệ số xác định (r (2)) liên quan đến hồi quy tuyến tính chỉ ra rằng đất sét có mối tương quan cao hơn với kiểu phân đoạn của PBDE so với các tính chất khác, chẳng hạn như SOC và phân đoạn hạt mịn. Các giá trị r (2) giữa đất sét và ba phân đoạn của PBDE là 0,70, 0,69 và 0,58."} +{"idx": 1990085, "en": "This paper addresses the effects of gaseous nitric acid (HNO(3)) and ozone (O(3)), two important air pollutants, on six lichen species with different morphological, ecological, and biological characteristics. The treatment chambers were set up in a factorial design consisting of control chambers, chambers fumigated with HNO(3), with O(3), and with HNO(3) and O(3), together. Each species showed a different sensitivity to the fumigations, reflecting the physiological variation among species. Our results clearly indicate that HNO(3) is a strong phytotoxin to many lichens, and that O(3) alone has little effect on the measured parameters. The combined fumigation effects of HNO(3) and O(3) were not significantly different from HNO(3) alone.", "gemini": "Bài báo này đề cập đến tác động của axit nitric dạng khí (HNO (3)) và ozone (O (3)), hai chất ô nhiễm không khí quan trọng, đối với sáu loài địa y có đặc điểm hình thái, sinh thái và sinh học khác nhau. Các buồng xử lý được thiết lập theo thiết kế giai thừa bao gồm buồng đối chứng, buồng hun khói bằng HNO (3), bằng O (3) và bằng HNO (3) và O (3) cùng nhau. Mỗi loài biểu hiện một độ nhạy khác nhau đối với quá trình hun khói, phản ánh sự khác biệt về mặt sinh lý giữa các loài. Kết quả của chúng tôi chỉ ra rõ ràng rằng HNO (3) là một chất độc thực vật mạnh đối với nhiều loài địa y và bản thân O (3) có ít tác động đến các thông số đã đo được. Các tác động hun khói kết hợp của HNO (3) và O (3) không khác biệt đáng kể so với riêng HNO (3)."} +{"idx": 1990086, "en": "The study investigated physiological responses during 2-km walking at a certain intensity of a previously performed maximal exercise test where moderate perceived exertion was reported. Twenty seniors were examined by an incremental walking treadmill test to obtain maximal oxygen uptake (VO2max). A submaximal 2-km walking test was applied 1 wk later. The corresponding moderate perceived exertion (4 on the CR-10 scale) during the VO2max test was applied to the 2-km treadmill test. Moderate exertion (mean rating of perceived exertion [RPE]: 4 ± 1) led to 76% ± 8% of VO2max and 79% ± 6% of maximal heart rate. RPE values drifted with a significant time effect (p = .001, η(p) = .58) during the 2-km test from 3 ± 0.7 to 4.6 ± 0.8. Total energy expenditure (EE) was 3.3 ± 0.5 kcal/kg. No gender differences in ventilatory, heart-rate, or EE data occurred. Brisk walking at moderate RPE of 3-5 would lead to a beneficial physiological response during endurance training and a weekly EE of nearly 1,200 kcal when exercising 5 times/wk for 30 min.", "gemini": "Nghiên cứu đã điều tra các phản ứng sinh lý trong quá trình đi bộ 2 km ở một cường độ nhất định của bài kiểm tra gắng sức tối đa đã thực hiện trước đó, trong đó có báo cáo về gắng sức cảm nhận được ở mức độ vừa phải. Hai mươi người cao tuổi đã được kiểm tra bằng bài kiểm tra đi bộ trên máy chạy bộ gia tăng để có được lượng oxy hấp thụ tối đa (VO2max). Một bài kiểm tra đi bộ dưới tối đa 2 km đã được áp dụng sau 1 tuần. Nỗ lực cảm nhận được ở mức độ vừa phải tương ứng (4 trên thang CR-10) trong bài kiểm tra VO2max đã được áp dụng cho bài kiểm tra máy chạy bộ 2 km. Nỗ lực vừa phải (đánh giá trung bình về nỗ lực cảm nhận được [RPE]: 4 ± 1) dẫn đến 76% ± 8% VO2max và 79% ± 6% nhịp tim tối đa. Các giá trị RPE thay đổi theo thời gian có ý nghĩa (p = .001, η (p) = .58) trong bài kiểm tra 2 km từ 3 ± 0,7 đến 4,6 ± 0,8. Tổng năng lượng tiêu hao (EE) là 3,3 ± 0,5 kcal / kg. Không có sự khác biệt về giới tính trong dữ liệu về thông khí, nhịp tim hoặc EE. Đi bộ nhanh với RPE vừa phải từ 3-5 sẽ dẫn đến phản ứng sinh lý có lợi trong quá trình luyện tập sức bền và EE hàng tuần gần 1.200 kcal khi tập thể dục 5 lần / tuần trong 30 phút."} +{"idx": 1990087, "en": "This paper investigates the extent of socioeconomic inequalities in antenatal care use and related medical procedures in Brazil and India, which represent transition economies with contrasting geographical and sociocultural composition and health care provision. Concentration indices and regression analyses applied on recent Demographic Health Survey data reveal high and proportionate distribution of antenatal coverage in Brazil, whereas the Indian case present problems of both scale and equity. Inequalities in access to four or more antenatal visits are significantly pronounced in India, and in Brazil the differences are significant only for those who had six or more visits. Brazil's universal healthcare model which proved effective in promoting equitable distribution of antenatal care could be implemented in India. Future interventions should emphasis quality of care in monitoring essential antenatal services especially targeting the poor and deprived communities.", "gemini": "Bài báo này điều tra mức độ bất bình đẳng kinh tế xã hội trong việc sử dụng chăm sóc trước sinh và các thủ thuật y tế liên quan ở Brazil và Ấn Độ, đại diện cho các nền kinh tế chuyển đổi có thành phần địa lý, văn hóa xã hội và cung cấp dịch vụ chăm sóc sức khỏe tương phản. Các chỉ số tập trung và phân tích hồi quy được áp dụng trên dữ liệu Khảo sát sức khỏe nhân khẩu gần đây cho thấy sự phân bổ tỷ lệ thuận và cao về phạm vi chăm sóc trước sinh ở Brazil, trong khi trường hợp Ấn Độ nêu ra vấn đề về cả quy mô và công bằng. Sự bất bình đẳng trong việc tiếp cận bốn hoặc nhiều lần khám trước sinh được thể hiện rõ rệt ở Ấn Độ, và ở Brazil, sự khác biệt chỉ đáng kể đối với những người đã khám sáu lần trở lên. Mô hình chăm sóc sức khỏe toàn dân của Brazil đã chứng minh hiệu quả trong việc thúc đẩy phân phối công bằng dịch vụ chăm sóc trước sinh có thể được triển khai ở Ấn Độ. Các biện pháp can thiệp trong tương lai nên nhấn mạnh vào chất lượng chăm sóc trong việc theo dõi các dịch vụ chăm sóc trước sinh thiết yếu, đặc biệt là nhắm vào các cộng đồng nghèo và khó khăn."} +{"idx": 1990088, "en": "At present Denmark has no central database holding records of equine health and disease. Nor have attitudes to the establishment of a national database, and the concerns it raises, been investigated in a systematic way. The objective of the present study was to assess the attitudes and concerns of potential database stakeholders. Attitudes to participation, and the location, financing and management of the proposed database, together with any concerns it raises, were investigated in a questionnaire study of 13 potential stakeholder groups in Denmark (in total, 1581 questionnaire recipients and 717 respondents): equine veterinarians, researchers, veterinary students, animal welfare organization representatives, horse owners, trainers, farriers, representatives from authorities, ordinary citizens, and representatives of laboratories, and insurance, medical equipment and pharmaceutical companies. Proportions were calculated for pre-categorised responses, and supplementary attitudes were extracted from qualitative responses. Eighty-six percent of respondents stated a positive interest in providing data for the database; the percentage for veterinarians was 90%. Data contribution was regarded as feasible by many of the stakeholder groups; this willingness to provide data, however, depended on the implementation of a very user-friendly system. Requirements included an electronic, simple, and time-efficient data reporting system. Most respondents felt the database should be financed via horse owners through mandatory contributions linked to each horse. Disagreement appeared to arise over data ownership, accessibility, and location. These and other issues need further elaboration in order to find a solution that is acceptable for all stakeholders. It may be advisable to collect and store data in more than one database.", "gemini": "Hiện tại, Đan Mạch không có cơ sở dữ liệu trung tâm lưu giữ hồ sơ về sức khỏe và bệnh tật của ngựa. Thái độ đối với việc thành lập một cơ sở dữ liệu quốc gia và những lo ngại mà nó nêu ra cũng chưa được điều tra một cách có hệ thống. Mục tiêu của nghiên cứu hiện tại là đánh giá thái độ và mối quan tâm của các bên liên quan đến cơ sở dữ liệu tiềm năng. Thái độ đối với việc tham gia, địa điểm, tài chính và quản lý cơ sở dữ liệu được đề xuất, cùng với bất kỳ mối quan tâm nào mà nó nêu ra, đã được điều tra trong một nghiên cứu bảng câu hỏi của 13 nhóm bên liên quan tiềm năng tại Đan Mạch (tổng cộng là 1581 người nhận bảng câu hỏi và 717 người trả lời): bác sĩ thú y về ngựa, nhà nghiên cứu, sinh viên thú y, đại diện tổ chức phúc lợi động vật, chủ sở hữu ngựa, huấn luyện viên, thợ rèn, đại diện từ các cơ quan chức năng, công dân bình thường và đại diện của các phòng thí nghiệm, bảo hiểm, thiết bị y tế và các công ty dược phẩm. Tỷ lệ được tính cho các phản hồi được phân loại trước và các thái độ bổ sung được trích xuất từ các phản hồi định tính. Tám mươi sáu phần trăm số người được hỏi cho biết họ quan tâm tích cực đến việc cung cấp dữ liệu cho cơ sở dữ liệu; tỷ lệ phần trăm đối với bác sĩ thú y là 90%. Nhiều nhóm bên liên quan coi việc đóng góp dữ liệu là khả thi; tuy nhiên, sự sẵn lòng cung cấp dữ liệu này phụ thuộc vào việc triển khai một hệ thống rất thân thiện với người dùng. Các yêu cầu bao gồm hệ thống báo cáo dữ liệu điện tử, đơn giản và tiết kiệm thời gian. Hầu hết những người được hỏi đều cảm thấy cơ sở dữ liệu nên được tài trợ thông qua chủ sở hữu ngựa thông qua các khoản đóng góp bắt buộc liên quan đến từng con ngựa. Sự bất đồng dường như phát sinh xung quanh quyền sở hữu, khả năng truy cập và vị trí dữ liệu. Những vấn đề này và các vấn đề khác cần được giải quyết thêm để tìm ra giải pháp được tất cả các bên liên quan chấp nhận. Có thể nên thu thập và lưu trữ dữ liệu trong nhiều cơ sở dữ liệu."} +{"idx": 1990089, "en": "Hydroxylated polybrominated diphenyl ethers (HO-PBDEs) pose potential ecological risks due to their endocrine disrupting effects and extensive sources. It is of great importance to know their environmental transformation for the purpose of ecological risk assessment. Photodegradation is an important transformation pathway of HO-PBDEs. As HO-PBDEs ionize in natural waters, the photochemical reactivities of both neutral and anionic HO-PBDEs need to be unveiled. In this study, six HO-PBDEs were selected as model compounds. Their direct photolysis rate constants (k(d)), quantum yields (Φ), light absorptions (A), second-order reaction rate constants with (1)O(2) (k(1O2)) and (·)OH (k(OH)) at different pH were determined, as well as these parameters for the neutral and anionic HO-PBDEs. The k(d), Φ, A, k(1O2) and k(OH) for the anions are much higher than those for the neutral molecules and vary with the bromination degree. Molecular parameters computed with the density functional theory (DFT) were employed to construct structure-reactivity equations. The ether bond strength, the frontier molecular orbital energy and the charge distribution were found to be the intrinsic structural characters governing the photochemical reactivities. The half-lives range from 0.7 to 60.1h for the photodegradation including the direct photolysis and the reactions with (1)O(2) and ()OH in surface waters at 45°N latitude under the continuous solar irradiation of sunny noon on 15 July. Direct photolysis is the dominant pathway. The photochemical reactivities of other HO-PBDEs at a given pH can also be estimated based on the structure-reactivity equations, which is important for the ecological risk assessment of HO-PBDEs.", "gemini": "Các ete diphenyl polybrom hóa hydroxyl hóa (HO-PBDE) gây ra những rủi ro sinh thái tiềm tàng do tác động phá vỡ nội tiết và các nguồn rộng lớn của chúng. Việc biết về sự chuyển đổi môi trường của chúng là rất quan trọng cho mục đích đánh giá rủi ro sinh thái. Phân hủy quang học là một con đường chuyển đổi quan trọng của HO-PBDE. Vì HO-PBDE ion hóa trong nước tự nhiên, nên phản ứng quang hóa của cả HO-PBDE trung tính và anion cần được làm sáng tỏ. Trong nghiên cứu này, sáu HO-PBDE được chọn làm hợp chất mô hình. Hằng số tốc độ quang phân trực tiếp của chúng (k(d)), năng suất lượng tử (Φ), hấp thụ ánh sáng (A), hằng số tốc độ phản ứng bậc hai với (1) O (2) (k (1O2)) và (·) OH (k (OH)) ở các giá trị pH khác nhau đã được xác định, cũng như các thông số này đối với HO-PBDE trung tính và anion. k (d), Φ, A, k (1O2) và k (OH) đối với các anion cao hơn nhiều so với các phân tử trung tính và thay đổi theo mức độ brom hóa. Các thông số phân tử được tính bằng lý thuyết hàm mật độ (DFT) được sử dụng để xây dựng các phương trình phản ứng cấu trúc. Cường độ liên kết ete, năng lượng quỹ đạo phân tử biên và phân bố điện tích được phát hiện là các đặc tính cấu trúc nội tại chi phối phản ứng quang hóa. Thời gian bán hủy dao động từ 0,7 đến 60,1 giờ đối với quá trình phân hủy quang học bao gồm cả quang phân trực tiếp và phản ứng với (1) O (2) và () OH trong nước mặt ở vĩ độ 45 ° Bắc dưới sự chiếu xạ liên tục của mặt trời vào buổi trưa ngày 15 tháng 7. Quang phân trực tiếp là con đường chiếm ưu thế. Phản ứng quang hóa của các HO-PBDE khác ở một giá trị pH nhất định cũng có thể được ước tính dựa trên các phương trình phản ứng cấu trúc, điều này rất quan trọng đối với việc đánh giá rủi ro sinh thái của HO-PBDE."} +{"idx": 1990090, "en": "In this study we evaluated the hyperglycemic and hyperlipidemic effects of chlorpyrifos (CPF) after an acute exposure in rats. The mechanisms involved in hyperglycemia induced by CPF were studied. A single dose of CPF (50 mg kg(-1), subcutaneous, s.c.) was administered to overnight-fasted rats. Glucose and corticosterone levels, lipid status and paraoxonase (PON1) activity were determined in plasma of rats. Cardiovascular risk factors and the atherogenic index were calculated. Glycogen levels, tyrosine aminotransferase (TAT) and glucose-6-phosphatase (G6Pase) activities were determined in livers of rats. Cerebral acetylcholinesterase (AChE) activity was also determined. CPF caused an increase in glucose and glycogen levels as well as in TAT and G6Pase activities. The CPF exposure caused an increase in corticosterone levels, an inhibition of AChE activity and a reduction of PON1 activity. Regarding the lipid status, CPF induced an increase in triglycerides (TG) and low-density lipoprotein-cholesterol (LDL) levels and a decrease in high-density lipoprotein (HDL) levels associated with an increase of cardiovascular risk factors and the atherogenic index. The present study demonstrated that a single CPF administration caused hyperglycemia and hyperlipidemia in rats. The activation of the gluconeogenesis pathway, probably elicited by hypercorticosteronemia, is involved in the hyperglycemic effect of CPF in rats.", "gemini": "Trong nghiên cứu này, chúng tôi đã đánh giá tác dụng tăng đường huyết và tăng lipid máu của chlorpyrifos (CPF) sau khi tiếp xúc cấp tính ở chuột. Các cơ chế liên quan đến tình trạng tăng đường huyết do CPF gây ra đã được nghiên cứu. Một liều duy nhất CPF (50 mg kg(-1), tiêm dưới da, s.c.) đã được dùng cho những con chuột nhịn ăn qua đêm. Nồng độ glucose và corticosterone, tình trạng lipid và hoạt động paraoxonase (PON1) đã được xác định trong huyết tương của chuột. Các yếu tố nguy cơ tim mạch và chỉ số gây xơ vữa động mạch đã được tính toán. Nồng độ glycogen, hoạt động của tyrosine aminotransferase (TAT) và glucose-6-phosphatase (G6Pase) đã được xác định trong gan của chuột. Hoạt động của acetylcholinesterase (AChE) ở não cũng đã được xác định. CPF gây ra sự gia tăng nồng độ glucose và glycogen cũng như hoạt động của TAT và G6Pase. Sự tiếp xúc với CPF gây ra sự gia tăng nồng độ corticosterone, ức chế hoạt động của AChE và làm giảm hoạt động của PON1. Về tình trạng lipid, CPF gây ra sự gia tăng nồng độ triglyceride (TG) và cholesterol lipoprotein tỷ trọng thấp (LDL) và làm giảm nồng độ lipoprotein tỷ trọng cao (HDL) liên quan đến sự gia tăng các yếu tố nguy cơ tim mạch và chỉ số gây xơ vữa động mạch. Nghiên cứu hiện tại chứng minh rằng một lần dùng CPF gây ra tình trạng tăng đường huyết và tăng lipid máu ở chuột. Sự kích hoạt của con đường tân đường niệu, có thể do tình trạng tăng tiết corticosterone gây ra, có liên quan đến tác dụng tăng đường huyết của CPF ở chuột."} +{"idx": 1990091, "en": "In order to characterize the effect of vegetation on performance of constructed wetlands (CWs) treating low and high chlorinated hydrocarbon, two pilot-scale horizontal subsurface flow (HSSF) CWs (planted with Phragmites australis and unplanted) treating sulphate rich groundwater contaminated with MCB (monochlorobenzene, as a low chlorinated hydrocarbon), (about 10 mg L(-1)), and PCE (perchloroethylene, as a high chlorinated hydrocarbon), (about 2 mg L(-1)), were examined. With mean MCB inflow load of 299 mg m(-2) d(-1), the removal rate was 58 and 208 mg m(-2) d(-1) in the unplanted and planted wetland, respectively, after 4 m from the inlet. PCE was almost completely removed in both wetlands with mean inflow load of 49 mg m(-2) d(-1). However, toxic metabolites cis-1,2-DCE (dichloroethene) and VC (vinyl chloride) accumulated in the unplanted wetland; up to 70% and 25% of PCE was dechlorinated to cis-1,2-DCE and VC after 4 m from the inlet, respectively. Because of high sulphate concentration (around 850 mg L(-1)) in the groundwater, the plant derived organic carbon caused sulphide formation (up to 15 mg L(-1)) in the planted wetland, which impaired the MCB removal but not statistically significant. The results showed significant enhancement of vegetation on the removal of the low chlorinated hydrocarbon MCB, which is probably due to the fact that aerobic MCB degraders are benefited from the oxygen released by plant roots. Vegetation also stimulated completely dechlorination of PCE due to plant derived organic carbon, which is potentially to provide electron donor for dechlorination process. The plant derived organic carbon also stimulated dissimilatory sulphate reduction, which subsequently have negative effect on MCB removal.", "gemini": "Để mô tả tác động của thảm thực vật đến hiệu suất của các vùng đất ngập nước nhân tạo (CW) xử lý hydrocarbon clo hóa ở mức thấp và cao, hai CW dòng chảy ngầm ngang quy mô thí điểm (HSSF) (trồng Phragmites australis và không trồng) xử lý nước ngầm giàu sulfat bị nhiễm MCB (monoclobenzen, là hydrocarbon clo hóa ở mức thấp), (khoảng 10 mg L(-1)) và PCE (perchloroetylen, là hydrocarbon clo hóa ở mức cao), (khoảng 2 mg L(-1)), đã được kiểm tra. Với lượng MCB vào trung bình là 299 mg m(-2) d(-1), tỷ lệ loại bỏ là 58 và 208 mg m(-2) d(-1) ở vùng đất ngập nước không trồng và có trồng, tương ứng, sau 4 m từ đầu vào. PCE đã được loại bỏ gần như hoàn toàn ở cả hai vùng đất ngập nước với lượng vào trung bình là 49 mg m(-2) d(-1). Tuy nhiên, các chất chuyển hóa độc hại cis-1,2-DCE (dichloroethene) và VC (vinyl clorua) tích tụ trong vùng đất ngập nước không trồng; lên đến 70% và 25% PCE đã bị khử clo thành cis-1,2-DCE và VC sau 4 m từ đầu vào, tương ứng. Do nồng độ sulfat cao (khoảng 850 mg L(-1)) trong nước ngầm, carbon hữu cơ có nguồn gốc từ thực vật gây ra sự hình thành sunfua (lên đến 15 mg L(-1)) trong vùng đất ngập nước có trồng, làm suy yếu quá trình loại bỏ MCB nhưng không có ý nghĩa thống kê. Kết quả cho thấy sự tăng cường đáng kể của thảm thực vật đối với việc loại bỏ hydrocarbon clo hóa ở mức thấp MCB, có thể là do các chất phân hủy MCB hiếu khí được hưởng lợi từ oxy do rễ cây giải phóng. Thảm thực vật cũng kích thích quá trình khử clo hoàn toàn PCE do carbon hữu cơ có nguồn gốc từ thực vật, có khả năng cung cấp chất cho điện tử cho quá trình khử clo. Carbon hữu cơ có nguồn gốc từ thực vật cũng kích thích quá trình khử sunfat không đồng hóa, sau đó có tác động tiêu cực đến quá trình loại bỏ MCB."} +{"idx": 1990092, "en": "Few currently used agricultural pesticides are routinely monitored for in the environment. Even if concentrations are known, sediment LC(50) values are often lacking for common sediment toxicity testing species. To help fill this data gap, sediments in California's Central Valley were tested for nine hydrophobic pesticides seldom analyzed: abamectin, diazinon, dicofol, fenpropathrin, indoxacarb, methyl parathion, oxyfluorfen, propargite, and pyraclostrobin. Most were detected, but rarely at concentrations acutely toxic to Hyalella azteca or Chironomus dilutus. Only abamectin, fenpropathrin, and methyl parathion were found at concentrations of potential concern, and only in one or two samples. One-quarter of over 100 samples from agriculture-affected waterways exhibited toxicity, and in three-fourths of the toxic samples, pyrethroids exceeded concentrations expected to cause toxicity. The pyrethroid Bi-fen-thrin in particular, as well as lambda-cyhalothrin, cypermethrin, esfenvalerate, permethrin, and the organophosphate chlorpyrifos, were primarily responsible for the observed toxicity, rather than the more novel analytes, despite the fact that much of the sampling targeted areas of greatest use of the novel pesticides.", "gemini": "Ít thuốc trừ sâu nông nghiệp hiện đang được sử dụng được theo dõi thường xuyên trong môi trường. Ngay cả khi biết nồng độ, các giá trị LC(50) của trầm tích thường không có đối với các loài thử độc tính trầm tích phổ biến. Để giúp lấp đầy khoảng trống dữ liệu này, các trầm tích ở Thung lũng Trung tâm California đã được thử nghiệm đối với chín loại thuốc trừ sâu kỵ nước hiếm khi được phân tích: abamectin, diazinon, dicofol, fenpropathrin, indoxacarb, methyl parathion, oxyfluorfen, propargite và pyraclostrobin. Hầu hết đều được phát hiện, nhưng hiếm khi ở nồng độ độc cấp tính đối với Hyalella azteca hoặc Chironomus dilutus. Chỉ có abamectin, fenpropathrin và methyl parathion được tìm thấy ở nồng độ đáng quan tâm và chỉ trong một hoặc hai mẫu. Một phần tư trong số hơn 100 mẫu từ các tuyến đường thủy bị ảnh hưởng bởi nông nghiệp đã biểu hiện độc tính và trong ba phần tư số mẫu độc hại, pyrethroid vượt quá nồng độ dự kiến gây độc. Đặc biệt là pyrethroid Bi-fen-thrin, cũng như lambda-cyhalothrin, cypermethrin, esfenvalerate, permethrin và chlorpyrifos organophosphat, là nguyên nhân chính gây ra độc tính quan sát được, chứ không phải các chất phân tích mới hơn, mặc dù thực tế là phần lớn các khu vực lấy mẫu là nơi sử dụng nhiều nhất các loại thuốc trừ sâu mới."} +{"idx": 1990093, "en": "Fish gills are target organs for waterborne metal ions and this work aimed to investigate the effects of waterborne Ni(2+) (10, 25 and 50 mg L(-1)) on goldfish gills. A special focus was on the relationship between Ni uptake and the homeostasis of reactive oxygen species (ROS) in the gills, the tissue, in direct contact with the metal pollutant. Ni-accumulation in the gills occurred as a function of exposure concentration (R(2)=0.98). The main indices of oxidative stress, namely carbonyl proteins (CP) and lipid peroxides (LOOH), decreased by 21-33% and 21-24%, as well as the activities of principal antioxidant enzymes superoxide dismutase and glutathione-dependent peroxidase, by 29-47% and 41-46%, respectively, in gills of Ni-exposed fish. One of the main players in the antioxidant defense of gills seems to be catalase, which increased by 23-53% in Ni-treated fish, and low molecular mass thiol-containing compounds (L-SH), exceeding untreated controls by 73-105% after fish exposure to 10-50 mg L(-1) of Ni(2+). The increased level of L-SH, mainly represented by reduced glutathione, was supported by enhanced activities of glutathione reductase (by 27-38%), glutathione-S-transferase (56-141%) and glucose-6-phosphate dehydrogenase (by 96-117%) and demonstrates the ability of the antioxidant system of gills to resist Ni-induced oxidative stress.", "gemini": "Mang cá là cơ quan đích của các ion kim loại trong nước và công trình này nhằm mục đích nghiên cứu các tác động của Ni(2+) trong nước (10, 25 và 50 mg L(-1)) lên mang cá vàng. Trọng tâm đặc biệt là mối quan hệ giữa sự hấp thụ Ni và cân bằng nội môi của các loài oxy phản ứng (ROS) trong mang, mô tiếp xúc trực tiếp với kim loại ô nhiễm. Sự tích tụ Ni trong mang xảy ra như một hàm của nồng độ phơi nhiễm (R(2)=0,98). Các chỉ số chính của stress oxy hóa, cụ thể là protein carbonyl (CP) và lipid peroxide (LOOH), giảm 21-33% và 21-24%, cũng như hoạt động của các enzym chống oxy hóa chính là superoxide dismutase và glutathione-dependent peroxidase, giảm lần lượt 29-47% và 41-46% trong mang của cá tiếp xúc với Ni. Một trong những tác nhân chính trong cơ chế chống oxy hóa của mang dường như là catalase, tăng 23-53% ở cá được xử lý bằng Ni và các hợp chất chứa thiol có khối lượng phân tử thấp (L-SH), vượt quá đối chứng không xử lý 73-105% sau khi cá tiếp xúc với 10-50 mg L(-1) Ni(2+). Mức L-SH tăng, chủ yếu được biểu hiện bằng glutathione khử, được hỗ trợ bởi hoạt động tăng cường của glutathione reductase (27-38%), glutathione-S-transferase (56-141%) và glucose-6-phosphate dehydrogenase (96-117%) và chứng minh khả năng của hệ thống chống oxy hóa của mang để chống lại stress oxy hóa do Ni gây ra."} +{"idx": 1990094, "en": "We report a 35-year-old woman presenting a stroke-like episode with transitory aphasia followed by generalized tonic-clonic seizures. She had severe hearing loss and suffered from frequent episodes of migraine. Although a brain MRI disclosed a T2-hyperintense lesion in the left parietal lobe, she had hardly any long-term sequela. Exercise intolerance, myalgias and limb-girdle muscle weakness indicated a slowly progressive myopathy. Extra-neurological features included short stature, and secondary amenorrhea with low gonadotropin levels, indicating secondary hypogonadism. However, she had three mutation-free, healthy children by ovarian stimulation. A muscle biopsy showed ragged-red, cytochrome c oxidase-negative fibers, and an isolated defect of cytochrome c oxidase activity in muscle mitochondria. Sequence analysis of muscle mtDNA revealed a previously unreported heteroplasmic m.6597C>A transversion in the MTCOI gene, encoding subunit I of cytochrome c oxidase, corresponding to p.Q232K aminoacid change. Analysis on transmitochondrial cybrids demonstrated that the mutation is indeed associated with COX deficiency, i.e. pathogenic.", "gemini": "Chúng tôi báo cáo một phụ nữ 35 tuổi biểu hiện một cơn giống như đột quỵ với chứng mất ngôn ngữ thoáng qua sau đó là các cơn co giật toàn thể. Cô ấy bị mất thính lực nặng và thường xuyên bị chứng đau nửa đầu. Mặc dù chụp MRI não cho thấy tổn thương tăng tín hiệu T2 ở thùy đỉnh trái, nhưng cô ấy hầu như không có di chứng nào về lâu dài. Không dung nạp gắng sức, đau cơ và yếu cơ thắt lưng chi cho thấy bệnh cơ tiến triển chậm. Các đặc điểm ngoài thần kinh bao gồm vóc dáng thấp, và vô kinh thứ phát với nồng độ gonadotropin thấp, chỉ ra tình trạng suy sinh dục thứ phát. Tuy nhiên, cô đã có ba đứa con khỏe mạnh không đột biến bằng cách kích thích buồng trứng. Sinh thiết cơ cho thấy các sợi cơ bị rách, không có cytochrome c oxidase và một khiếm khuyết riêng biệt về hoạt động của cytochrome c oxidase trong ty thể cơ. Phân tích trình tự mtDNA cơ cho thấy một chuyển đoạn m.6597C>A dị hợp tử chưa được báo cáo trước đây trong gen MTCOI, mã hóa tiểu đơn vị I của cytochrome c oxidase, tương ứng với sự thay đổi axit amin p.Q232K. Phân tích trên các tế bào lai ty thể truyền dẫn chứng minh rằng đột biến thực sự liên quan đến tình trạng thiếu COX, tức là gây bệnh."} +{"idx": 1990095, "en": "Clinical experience supports a critical role for nutrition in patients with spinal muscular atrophy (SMA). Three-day dietary intake records were analyzed for 156 visits in 47 SMA type I patients, 25 males and 22 females, ages 1month to 13years (median 9.8months) and compared to dietary reference intakes for gender and age along with anthropometric measures and dual-energy X-ray absorptiometry (DEXA) data. Using standardized growth curves, twelve patients met criteria for failure to thrive (FTT) with weight for age <3rd percentile; eight met criteria based on weight for height. Percentage of body fat mass was not correlated with weight for height and weight for age across percentile categories. DEXA analysis further demonstrated that SMA type I children have higher fat mass and lower fat free mass than healthy peers (p<0.001). DEXA and dietary analysis indicates a strong correlation with magnesium intake and bone mineral density (r=0.65, p<0.001). Average caloric intake for 1-3years old was 68.8±15.8kcal/kg - 67% of peers' recommended intake. Children with SMA type I may have lower caloric requirements than healthy age-matched peers, increasing risk for over and undernourished states and deficiencies of critical nutrients. Standardized growth charts may overestimate FTT status in SMA type I.", "gemini": "Kinh nghiệm lâm sàng chứng minh vai trò quan trọng của dinh dưỡng đối với bệnh nhân teo cơ tủy sống (SMA). Hồ sơ lượng thức ăn nạp vào trong ba ngày đã được phân tích cho 156 lần khám ở 47 bệnh nhân SMA typ I, 25 nam và 22 nữ, độ tuổi từ 1 tháng đến 13 tuổi (trung vị 9,8 tháng) và so sánh với lượng thức ăn tham khảo theo giới tính và độ tuổi cùng với các biện pháp nhân trắc học và dữ liệu đo hấp thụ tia X năng lượng kép (DEXA). Sử dụng các đường cong tăng trưởng chuẩn hóa, mười hai bệnh nhân đáp ứng các tiêu chí không phát triển (FTT) với cân nặng theo tuổi < phần trăm thứ 3; tám bệnh nhân đáp ứng các tiêu chí dựa trên cân nặng theo chiều cao. Tỷ lệ phần trăm khối lượng mỡ cơ thể không tương quan với cân nặng theo chiều cao và cân nặng theo tuổi trên các loại phần trăm. Phân tích DEXA tiếp tục chứng minh rằng trẻ em SMA typ I có khối lượng mỡ cao hơn và khối lượng không có mỡ thấp hơn so với những trẻ khỏe mạnh cùng lứa tuổi (p < 0,001). Phân tích DEXA và chế độ ăn uống cho thấy có mối tương quan mạnh với lượng magie nạp vào và mật độ khoáng xương (r = 0,65, p < 0,001). Lượng calo trung bình nạp vào đối với trẻ từ 1-3 tuổi là 68,8 ± 15,8 kcal/kg - 67% lượng khuyến nghị cho trẻ cùng lứa tuổi. Trẻ em mắc SMA typ I có thể có nhu cầu calo thấp hơn so với trẻ khỏe mạnh cùng lứa tuổi, làm tăng nguy cơ thiếu hụt và thừa dinh dưỡng cũng như thiếu các chất dinh dưỡng quan trọng. Biểu đồ tăng trưởng chuẩn hóa có thể đánh giá quá cao tình trạng FTT ở SMA typ I."} +{"idx": 1990096, "en": "The main diagnostic feature of congenital fibre type disproportion is that type 1 fibres are consistently smaller than type 2 fibres in the absence of other histological abnormalities. Mutations in the TPM3, RYR1 and ACTA1 genes are the most common established genetic causes. There has been one previous report of congenital fibre type disproportion due to a mutation in TPM2, although some atypical histological features were present. We present two cases in which novel de novo missense mutations in TPM2 are associated with marked fibre size disproportion. The finding of typical histological changes of congenital fibre type disproportion in association with a p.Ser61Pro mutation confirms that TPM2 can cause typical congenital fibre type disproportion. Although not seen on light microscopy studies, protein inclusions typical of small 'caps' were found on electron microscopy in a second patient with a p.Ala155Val mutation in TPM2. This case emphasises the importance of electron microscopy in patients with presumed congenital fibre type disproportion, to exclude the presence of caps, nemaline bodies or minicores, which, if present, may be very helpful in guiding genetic analysis.", "gemini": "Đặc điểm chẩn đoán chính của tình trạng mất cân đối loại sợi bẩm sinh là các sợi loại 1 luôn nhỏ hơn các sợi loại 2 khi không có các bất thường về mô học khác. Các đột biến ở gen TPM3, RYR1 và ACTA1 là những nguyên nhân di truyền phổ biến nhất đã được xác định. Đã có một báo cáo trước đây về tình trạng mất cân đối loại sợi bẩm sinh do đột biến ở TPM2, mặc dù một số đặc điểm mô học không điển hình đã có mặt. Chúng tôi trình bày hai trường hợp đột biến sai nghĩa mới xuất hiện ở TPM2 có liên quan đến tình trạng mất cân đối kích thước sợi rõ rệt. Phát hiện thấy những thay đổi mô học điển hình của tình trạng mất cân đối loại sợi bẩm sinh liên quan đến đột biến p.Ser61Pro xác nhận rằng TPM2 có thể gây ra tình trạng mất cân đối loại sợi bẩm sinh điển hình. Mặc dù không nhìn thấy trên các nghiên cứu kính hiển vi quang học, nhưng các thể vùi protein điển hình của các \"nắp\" nhỏ đã được tìm thấy trên kính hiển vi điện tử ở một bệnh nhân thứ hai có đột biến p.Ala155Val ở TPM2. Trường hợp này nhấn mạnh tầm quan trọng của kính hiển vi điện tử ở những bệnh nhân được cho là bị mất cân đối loại sợi bẩm sinh, để loại trừ sự hiện diện của nắp, thể nemaline hoặc nhân nhỏ, nếu có, có thể rất hữu ích trong việc hướng dẫn phân tích di truyền."} +{"idx": 1990097, "en": "The temperature-size rule (TSR) is an intraspecific phenomenon describing the phenotypic plastic response of an organism size to the temperature: individuals reared at cooler temperatures mature to be larger adults than those reared at warmer temperatures. The TSR is ubiquitous, affecting >80% species including uni- and multicellular groups. How the TSR is established has received attention in multicellular organisms, but not in unicells. Further, conceptual models suggest the mechanism of size change to be different in these two groups. Here, we test these theories using the protist Cyclidium glaucoma. We measure cell sizes, along with population growth during temperature acclimation, to determine how and when the temperature-size changes are achieved. We show that mother and daughter sizes become temporarily decoupled from the ratio 2:1 during acclimation, but these return to their coupled state (where daughter cells are half the size of the mother cell) once acclimated. Thermal acclimation is rapid, being completed within approximately a single generation. Further, we examine the impact of increased temperatures on carrying capacity and total biomass, to investigate potential adaptive strategies of size change. We demonstrate no temperature effect on carrying capacity, but maximum supported biomass to decrease with increasing temperature.", "gemini": "Quy tắc nhiệt độ-kích thước (TSR) là một hiện tượng trong loài mô tả phản ứng dẻo kiểu hình của kích thước cơ thể với nhiệt độ: những cá thể được nuôi ở nhiệt độ mát hơn sẽ trưởng thành thành những con trưởng thành lớn hơn những con được nuôi ở nhiệt độ ấm hơn. TSR có ở khắp mọi nơi, ảnh hưởng đến >80% các loài bao gồm cả các nhóm đơn bào và đa bào. Cách thức TSR được thiết lập đã nhận được sự chú ý ở các sinh vật đa bào, nhưng không phải ở các sinh vật đơn bào. Hơn nữa, các mô hình khái niệm cho thấy cơ chế thay đổi kích thước ở hai nhóm này là khác nhau. Ở đây, chúng tôi kiểm tra các lý thuyết này bằng cách sử dụng sinh vật đơn bào Cyclidium glaucoma. Chúng tôi đo kích thước tế bào, cùng với sự tăng trưởng của quần thể trong quá trình thích nghi với nhiệt độ, để xác định cách thức và thời điểm đạt được những thay đổi về nhiệt độ-kích thước. Chúng tôi chỉ ra rằng kích thước của mẹ và con gái tạm thời không còn tỷ lệ 2:1 trong quá trình thích nghi, nhưng chúng trở lại trạng thái ghép nối của chúng (nơi các tế bào con có kích thước bằng một nửa tế bào mẹ) sau khi thích nghi. Sự thích nghi với nhiệt diễn ra nhanh chóng, hoàn thành trong khoảng một thế hệ. Hơn nữa, chúng tôi kiểm tra tác động của nhiệt độ tăng lên khả năng chịu đựng và tổng sinh khối, để nghiên cứu các chiến lược thích nghi tiềm năng của sự thay đổi kích thước. Chúng tôi chứng minh không có tác động của nhiệt độ đối với khả năng chịu đựng, nhưng sinh khối tối đa được hỗ trợ giảm khi nhiệt độ tăng."} +{"idx": 1990098, "en": "Hutchinson's fundamental niche, defined by the physical and biological environments in which an organism can thrive in the absence of inter-species interactions, is an important theoretical concept in ecology. However, little is known about the overlap between the fundamental niche and the set of conditions species inhabit in nature, and about natural variation in fundamental niche shape and its change as species adapt to their environment. Here, we develop a custom-made dual gradient apparatus to map a cross-section of the fundamental niche for several marine bacterial species within the genus Vibrio based on their temperature and salinity tolerance, and compare tolerance limits to the environment where these species commonly occur. We interpret these niche shapes in light of a conceptual model comprising five basic niche shapes. We find that the fundamental niche encompasses a much wider set of conditions than those strains typically inhabit, especially for salinity. Moreover, though the conditions that strains typically inhabit agree well with the strains' temperature tolerance, they are negatively correlated with the strains' salinity tolerance. Such relationships can arise when the physiological response to different stressors is coupled, and we present evidence for such a coupling between temperature and salinity tolerance. Finally, comparison with well-documented ecological range in V. vulnificus suggests that biotic interactions limit the occurrence of this species at low-temperature-high-salinity conditions. Our findings highlight the complex interplay between the ecological, physiological and evolutionary determinants of niche morphology, and caution against making inferences based on a single ecological factor.", "gemini": "Ngách cơ bản của Hutchinson, được xác định bởi các môi trường vật lý và sinh học mà một sinh vật có thể phát triển mạnh trong điều kiện không có tương tác giữa các loài, là một khái niệm lý thuyết quan trọng trong sinh thái học. Tuy nhiên, người ta biết rất ít về sự chồng lấn giữa ngách cơ bản và tập hợp các điều kiện mà các loài cư trú trong tự nhiên, và về sự biến đổi tự nhiên về hình dạng ngách cơ bản và sự thay đổi của nó khi các loài thích nghi với môi trường của chúng. Ở đây, chúng tôi phát triển một thiết bị độ dốc kép được thiết kế riêng để lập bản đồ một mặt cắt ngang của ngách cơ bản cho một số loài vi khuẩn biển trong chi Vibrio dựa trên khả năng chịu nhiệt độ và độ mặn của chúng, và so sánh giới hạn chịu đựng với môi trường mà các loài này thường xuất hiện. Chúng tôi diễn giải những hình dạng ngách này dưới góc độ của một mô hình khái niệm bao gồm năm hình dạng ngách cơ bản. Chúng tôi thấy rằng ngách cơ bản bao gồm một tập hợp các điều kiện rộng hơn nhiều so với những chủng thường sinh sống, đặc biệt là đối với độ mặn. Hơn nữa, mặc dù các điều kiện mà các chủng thường sinh sống phù hợp với khả năng chịu nhiệt của các chủng, nhưng chúng lại tương quan nghịch với khả năng chịu mặn của các chủng. Những mối quan hệ như vậy có thể phát sinh khi phản ứng sinh lý đối với các tác nhân gây căng thẳng khác nhau được kết hợp, và chúng tôi đưa ra bằng chứng cho sự kết hợp như vậy giữa khả năng chịu nhiệt và độ mặn. Cuối cùng, so sánh với phạm vi sinh thái được ghi chép rõ ràng ở V. vulnificus cho thấy rằng các tương tác sinh học hạn chế sự xuất hiện của loài này ở điều kiện nhiệt độ thấp-độ mặn cao. Những phát hiện của chúng tôi làm nổi bật sự tương tác phức tạp giữa các yếu tố quyết định về hình thái ngách sinh thái, sinh lý và tiến hóa, và cảnh báo không nên đưa ra suy luận dựa trên một yếu tố sinh thái duy nhất."} +{"idx": 1990102, "en": "Mild cognitive impairment (MCI) is considered as a transition phase between normal aging and Alzheimer's disease (AD). MCI confers an increased risk of developing AD, although the state is heterogeneous with several possible outcomes, including even improvement back to normal cognition. We sought to determine the serum metabolomic profiles associated with progression to and diagnosis of AD in a prospective study. At the baseline assessment, the subjects enrolled in the study were classified into three diagnostic groups: healthy controls (n=46), MCI (n=143) and AD (n=47). Among the MCI subjects, 52 progressed to AD in the follow-up. Comprehensive metabolomics approach was applied to analyze baseline serum samples and to associate the metabolite profiles with the diagnosis at baseline and in the follow-up. At baseline, AD patients were characterized by diminished ether phospholipids, phosphatidylcholines, sphingomyelins and sterols. A molecular signature comprising three metabolites was identified, which was predictive of progression to AD in the follow-up. The major contributor to the predictive model was 2,4-dihydroxybutanoic acid, which was upregulated in AD progressors (P=0.0048), indicating potential involvement of hypoxia in the early AD pathogenesis. This was supported by the pathway analysis of metabolomics data, which identified upregulation of pentose phosphate pathway in patients who later progressed to AD. Together, our findings primarily implicate hypoxia, oxidative stress, as well as membrane lipid remodeling in progression to AD. Establishment of pathogenic relevance of predictive biomarkers such as ours may not only facilitate early diagnosis, but may also help identify new therapeutic avenues.", "gemini": "Suy giảm nhận thức nhẹ (MCI) được coi là giai đoạn chuyển tiếp giữa quá trình lão hóa bình thường và bệnh Alzheimer (AD). MCI làm tăng nguy cơ mắc AD, mặc dù tình trạng này không đồng nhất với một số kết quả có thể xảy ra, bao gồm cả sự cải thiện trở lại nhận thức bình thường. Chúng tôi tìm cách xác định các cấu hình chuyển hóa huyết thanh liên quan đến tiến triển và chẩn đoán AD trong một nghiên cứu triển vọng. Khi đánh giá ban đầu, những đối tượng tham gia nghiên cứu được phân loại thành ba nhóm chẩn đoán: nhóm đối chứng khỏe mạnh (n = 46), MCI (n = 143) và AD (n = 47). Trong số các đối tượng MCI, 52 người tiến triển thành AD trong quá trình theo dõi. Phương pháp chuyển hóa toàn diện đã được áp dụng để phân tích các mẫu huyết thanh ban đầu và liên kết các cấu hình chuyển hóa với chẩn đoán ban đầu và trong quá trình theo dõi. Ở giai đoạn đầu, bệnh nhân AD được đặc trưng bởi giảm phospholipid ether, phosphatidylcholine, sphingomyelin và sterol. Một dấu hiệu phân tử bao gồm ba chất chuyển hóa đã được xác định, có khả năng dự đoán tiến triển thành AD trong quá trình theo dõi. Đóng góp chính cho mô hình dự đoán là axit 2,4-dihydroxybutanoic, được điều chỉnh tăng ở những người tiến triển thành AD (P = 0,0048), chỉ ra sự liên quan tiềm ẩn của tình trạng thiếu oxy trong quá trình sinh bệnh AD sớm. Điều này được hỗ trợ bởi phân tích con đường của dữ liệu chuyển hóa, xác định sự điều chỉnh tăng của con đường pentose phosphate ở những bệnh nhân sau này tiến triển thành AD. Cùng nhau, những phát hiện của chúng tôi chủ yếu liên quan đến tình trạng thiếu oxy, stress oxy hóa, cũng như tái tạo lipid màng trong quá trình tiến triển thành AD. Việc thiết lập sự liên quan về mặt bệnh sinh của các dấu ấn sinh học dự đoán như của chúng tôi không chỉ có thể tạo điều kiện thuận lợi cho việc chẩn đoán sớm mà còn có thể giúp xác định các phương pháp điều trị mới."} +{"idx": 1990103, "en": "Currently used antidepressants elevate monoamine levels in the synaptic cleft. There is good reason to assume that this is not the only source for antidepressant therapeutic activities and that secondary downstream effects may be relevant for alleviating symptoms of depression. We attempted to elucidate affected biochemical pathways downstream of monoamine reuptake inhibition by interrogating metabolomic profiles in DBA/2Ola mice after chronic paroxetine treatment. Metabolomic changes were investigated using gas chromatography-mass spectrometry profiling and group differences were analyzed by univariate and multivariate statistics. Pathways affected by antidepressant treatment were related to energy metabolism, amino acid metabolism and hormone signaling. The identified pathways reveal further antidepressant therapeutic action and represent targets for drug development efforts. A comparison of the central nervous system with blood plasma metabolite alterations identified GABA, galactose-6-phosphate and leucine as biomarker candidates for assessment of antidepressant treatment effects in the periphery.", "gemini": "Thuốc chống trầm cảm hiện đang sử dụng làm tăng nồng độ monoamin trong khe synap. Có lý do chính đáng để cho rằng đây không phải là nguồn duy nhất cho các hoạt động điều trị chống trầm cảm và các tác dụng phụ thứ cấp có thể có liên quan đến việc làm giảm các triệu chứng trầm cảm. Chúng tôi đã cố gắng làm sáng tỏ các con đường sinh hóa bị ảnh hưởng sau khi ức chế tái hấp thu monoamin bằng cách thẩm vấn các cấu hình chuyển hóa ở chuột DBA / 2Ola sau khi điều trị paroxetine mạn tính. Các thay đổi chuyển hóa đã được nghiên cứu bằng cách lập hồ sơ quang phổ khối sắc ký khí và các nhóm khác biệt đã được phân tích bằng thống kê đơn biến và đa biến. Các con đường bị ảnh hưởng bởi điều trị bằng thuốc chống trầm cảm có liên quan đến quá trình trao đổi chất năng lượng, quá trình trao đổi chất axit amin và tín hiệu hormone. Các con đường được xác định cho thấy tác dụng điều trị chống trầm cảm xa hơn và đại diện cho các mục tiêu cho các nỗ lực phát triển thuốc. So sánh hệ thần kinh trung ương với các biến đổi chuyển hóa huyết tương cho thấy GABA, galactose-6-phosphate và leucine là các ứng cử viên dấu ấn sinh học để đánh giá hiệu quả điều trị chống trầm cảm ở ngoại vi."} +{"idx": 1990104, "en": "Childhood maltreatment, through epigenetic modification of the glucocorticoid receptor gene (NR3C1), influences the hypothalamic-pituitary-adrenal axis (HPA axis). We investigated whether childhood maltreatment and its severity were associated with increased methylation of the exon 1(F) NR3C1 promoter, in 101 borderline personality disorder (BPD) and 99 major depressive disorder (MDD) subjects with, respectively, a high and low rate of childhood maltreatment, and 15 MDD subjects with comorbid post-traumatic stress disorder (PTSD). Childhood sexual abuse, its severity and the number of type of maltreatments positively correlated with NR3C1 methylation (P=6.16 × 10(-8), 5.18 × 10(-7) and 1.25 × 10(-9), respectively). In BPD, repetition of abuses and sexual abuse with penetration correlated with a higher methylation percentage. Peripheral blood might therefore serve as a proxy for environmental effects on epigenetic processes. These findings suggest that early life events may permanently impact on the HPA axis though epigenetic modifications of the NR3C1. This is a mechanism by which childhood maltreatment may lead to adulthood psychopathology.", "gemini": "Việc ngược đãi trẻ em, thông qua sửa đổi biểu sinh của gen thụ thể glucocorticoid (NR3C1), ảnh hưởng đến trục hạ đồi-tuyến yên-thượng thận (trục HPA). Chúng tôi đã điều tra xem việc ngược đãi trẻ em và mức độ nghiêm trọng của nó có liên quan đến việc tăng methyl hóa vùng khởi động exon 1 (F) NR3C1 hay không, ở 101 đối tượng rối loạn nhân cách ranh giới (BPD) và 99 đối tượng mắc chứng rối loạn trầm cảm chính (MDD), tương ứng với tỷ lệ ngược đãi trẻ em cao và thấp, và 15 đối tượng MDD mắc chứng rối loạn căng thẳng sau sang chấn (PTSD) đồng mắc. Lạm dụng tình dục trẻ em, mức độ nghiêm trọng của nó và số loại ngược đãi có tương quan thuận với mức độ methyl hóa NR3C1 (P = 6,16 × 10 (-8), 5,18 × 10 (-7) và 1,25 × 10 (-9), tương ứng). Ở BPD, việc lặp lại hành vi lạm dụng và lạm dụng tình dục có xâm nhập có tương quan với tỷ lệ methyl hóa cao hơn. Do đó, máu ngoại vi có thể đóng vai trò là sự thay thế cho các tác động của môi trường đối với các quá trình biểu sinh. Những phát hiện này cho thấy các sự kiện đầu đời có thể ảnh hưởng vĩnh viễn đến trục HPA thông qua các sửa đổi biểu sinh của NR3C1. Đây là cơ chế mà ngược đãi trẻ em có thể dẫn đến bệnh lý tâm thần ở tuổi trưởng thành."} +{"idx": 1990105, "en": "Stem cell-based regenerative therapy is considered a promising cellular therapeutic approach for the patients with incurable brain diseases. Mesenchymal stem cells (MSCs) represent an attractive cell source for regenerative medicine strategies for the treatment of the diseased brain. Previous studies have shown that these cells improve behavioral deficits in animal models of neurological disorders such as Parkinson's and Huntington's diseases. In the current study, we examined the capability of intracerebral human MSCs transplantation (medial pre-frontal cortex) to prevent the social impairment displayed by mice after withdrawal from daily phencyclidine (PCP) administration (10 mg kg(-1) daily for 14 days). Our results show that MSCs transplantation significantly prevented the PCP-induced social deficit, as assessed by the social preference test. In contrast, the PCP-induced social impairment was not modified by daily clozapine treatment. Tissue analysis revealed that the human MSCs survived in the mouse brain throughout the course of the experiment (23 days). Significantly increased cortical brain-derived neurotrophic factor levels were observed in the MSCs-treated group as compared with sham-operated controls. Furthermore, western blot analysis revealed that the ratio of phosphorylated Akt to Akt was significantly elevated in the MSCs-treated mice compared with the sham controls. Our results demonstrate that intracerebral transplantation of MSCs is beneficial in attenuating the social deficits induced by sub-chronic PCP administration. We suggest a novel therapeutic approach for the treatment of schizophrenia-like negative symptoms in animal models of the disorder.", "gemini": "Liệu pháp tái tạo dựa trên tế bào gốc được coi là một phương pháp điều trị bằng tế bào đầy hứa hẹn cho những bệnh nhân mắc các bệnh não không thể chữa khỏi. Tế bào gốc trung mô (MSC) đại diện cho một nguồn tế bào hấp dẫn cho các chiến lược y học tái tạo để điều trị não bị bệnh. Các nghiên cứu trước đây đã chỉ ra rằng những tế bào này cải thiện các khiếm khuyết về hành vi ở các mô hình động vật mắc các rối loạn thần kinh như bệnh Parkinson và bệnh Huntington. Trong nghiên cứu hiện tại, chúng tôi đã kiểm tra khả năng cấy ghép MSCs của người vào não (vỏ não trước trán giữa) để ngăn ngừa tình trạng suy giảm xã hội ở chuột sau khi cai phencyclidine (PCP) hàng ngày (10 mg kg(-1) hàng ngày trong 14 ngày). Kết quả của chúng tôi cho thấy cấy ghép MSCs ngăn ngừa đáng kể tình trạng thiếu hụt xã hội do PCP gây ra, được đánh giá bằng bài kiểm tra sở thích xã hội. Ngược lại, tình trạng suy giảm xã hội do PCP gây ra không bị thay đổi bởi điều trị clozapine hàng ngày. Phân tích mô cho thấy MSCs của người sống sót trong não chuột trong suốt quá trình thử nghiệm (23 ngày). Nồng độ yếu tố dinh dưỡng thần kinh có nguồn gốc từ vỏ não tăng lên đáng kể được quan sát thấy ở nhóm được điều trị bằng MSCs so với nhóm đối chứng giả phẫu. Hơn nữa, phân tích vết thấm cho thấy tỷ lệ Akt phosphoryl hóa so với Akt tăng lên đáng kể ở những con chuột được điều trị bằng MSCs so với nhóm đối chứng giả. Kết quả của chúng tôi chứng minh rằng cấy ghép MSCs vào não có lợi trong việc làm giảm các khiếm khuyết xã hội do dùng PCP mạn tính gây ra. Chúng tôi đề xuất một phương pháp điều trị mới để điều trị các triệu chứng tiêu cực giống như tâm thần phân liệt ở các mô hình động vật của rối loạn này."} +{"idx": 1990106, "en": "Mineralocorticoid (MR) and glucocorticoid receptors (GR) are abundantly expressed in the limbic brain and mediate cortisol effects on the stress-response and behavioral adaptation. Dysregulation of the stress response impairs adaptation and is a risk factor for depression, which is twice as abundant in women than in men. Because of the importance of MR for appraisal processes underlying the initial phase of the stress response we investigated whether specific MR haplotypes were associated with personality traits that predict the risk of depression. We discovered a common gene variant (haplotype 2, frequency ∼0.38) resulting in enhanced MR activity. Haplotype 2 was associated with heightened dispositional optimism in study 1 and with less hopelessness and rumination in study 2. Using data from a large genome-wide association study we then established that haplotype 2 was associated with a lower risk of depression. Interestingly, all effects were restricted to women. We propose that common functional MR haplotypes are important determinants of inter-individual variability in resilience to depression in women by differentially mediating cortisol effects on the stress system.", "gemini": "Các thụ thể mineralocorticoid (MR) và glucocorticoid (GR) được biểu hiện rất nhiều trong não vùng limbic và trung gian tác dụng của cortisol đối với phản ứng căng thẳng và thích nghi hành vi. Rối loạn điều hòa phản ứng căng thẳng làm suy yếu khả năng thích nghi và là yếu tố nguy cơ gây trầm cảm, phổ biến gấp đôi ở phụ nữ so với nam giới. Do tầm quan trọng của MR đối với các quá trình đánh giá cơ sở cho giai đoạn đầu của phản ứng căng thẳng, chúng tôi đã điều tra xem các kiểu gen MR cụ thể có liên quan đến các đặc điểm tính cách dự đoán nguy cơ trầm cảm hay không. Chúng tôi phát hiện ra một biến thể gen phổ biến (kiểu gen 2, tần số ∼0,38) dẫn đến hoạt động MR tăng cường. Kiểu gen 2 có liên quan đến khuynh hướng lạc quan cao hơn trong nghiên cứu 1 và ít tuyệt vọng và suy nghĩ tiêu cực hơn trong nghiên cứu 2. Sử dụng dữ liệu từ một nghiên cứu liên kết toàn bộ hệ gen lớn, sau đó chúng tôi thiết lập rằng kiểu gen 2 có liên quan đến nguy cơ trầm cảm thấp hơn. Điều thú vị là tất cả các tác động đều chỉ giới hạn ở phụ nữ. Chúng tôi đề xuất rằng các kiểu gen MR chức năng phổ biến là những yếu tố quyết định quan trọng đối với sự thay đổi giữa các cá thể về khả năng phục hồi đối với chứng trầm cảm ở phụ nữ bằng cách trung gian khác nhau tác dụng của cortisol lên hệ thống căng thẳng."} +{"idx": 1990107, "en": "The role of the immune system in neuropsychiatric diseases, including autism spectrum disorder (ASD), has long been hypothesized. This hypothesis has mainly been supported by family cohort studies and the immunological abnormalities found in ASD patients, but had limited findings in genetic association testing. Two cross-disorder genetic association tests were performed on the genome-wide data sets of ASD and six autoimmune disorders. In the polygenic score test, we examined whether ASD risk alleles with low effect sizes work collectively in specific autoimmune disorders and show significant association statistics. In the genetic variation score test, we tested whether allele-specific associations between ASD and autoimmune disorders can be found using nominally significant single-nucleotide polymorphisms. In both tests, we found that ASD is probabilistically linked to ankylosing spondylitis (AS) and multiple sclerosis (MS). Association coefficients showed that ASD and AS were positively associated, meaning that autism susceptibility alleles may have a similar collective effect in AS. The association coefficients were negative between ASD and MS. Significant associations between ASD and two autoimmune disorders were identified. This genetic association supports the idea that specific immunological abnormalities may underlie the etiology of autism, at least in a number of cases.", "gemini": "Vai trò của hệ thống miễn dịch trong các bệnh thần kinh, bao gồm rối loạn phổ tự kỷ (ASD), từ lâu đã được đưa ra giả thuyết. Giả thuyết này chủ yếu được hỗ trợ bởi các nghiên cứu nhóm gia đình và các bất thường về miễn dịch được tìm thấy ở những bệnh nhân mắc ASD, nhưng có những phát hiện hạn chế trong xét nghiệm liên kết di truyền. Hai xét nghiệm liên kết di truyền chéo rối loạn đã được thực hiện trên các tập dữ liệu toàn bộ hệ gen của ASD và sáu rối loạn tự miễn. Trong bài kiểm tra điểm số đa gen, chúng tôi đã kiểm tra xem các alen nguy cơ ASD có kích thước hiệu ứng thấp có hoạt động tập thể trong các rối loạn tự miễn cụ thể hay không và cho thấy số liệu thống kê liên kết đáng kể. Trong bài kiểm tra điểm số biến thể di truyền, chúng tôi đã kiểm tra xem có thể tìm thấy các liên kết đặc hiệu alen giữa ASD và các rối loạn tự miễn bằng cách sử dụng các đa hình nucleotide đơn có ý nghĩa danh nghĩa hay không. Trong cả hai thử nghiệm, chúng tôi thấy rằng ASD có khả năng liên quan đến viêm cột sống dính khớp (AS) và đa xơ cứng (MS). Hệ số liên kết cho thấy ASD và AS có liên quan tích cực, nghĩa là các alen nhạy cảm với tự kỷ có thể có tác dụng tập thể tương tự ở AS. Hệ số liên kết là âm giữa ASD và MS. Các mối liên quan đáng kể giữa ASD và hai rối loạn tự miễn đã được xác định. Liên kết di truyền này hỗ trợ cho ý tưởng rằng các bất thường về miễn dịch cụ thể có thể là nguyên nhân gây ra bệnh tự kỷ, ít nhất là trong một số trường hợp."} +{"idx": 1990108, "en": "Segmental testicular infarctions are a rare disease with a low prevalence and few cases have been reported in the literature. We present a 26-year-old male without any relevant medical history who consulted at the Urology Department due to mild pain in the right testicle over the last month. He had no previous trauma or acute testicular pain. Partial orchiectomy was performed through an inguinal incision with removal of lesions and reconstruction of the testicular parenchyma. Histological examination revealed testicular infarction with no presence of neoplastic cells.", "gemini": "Nhồi máu tinh hoàn theo từng phân đoạn là một bệnh hiếm gặp với tỷ lệ mắc thấp và có ít trường hợp được báo cáo trong tài liệu. Chúng tôi trình bày một nam giới 26 tuổi không có bất kỳ tiền sử bệnh lý nào có liên quan đã đến khám tại Khoa Tiết niệu do đau nhẹ ở tinh hoàn phải trong tháng vừa qua. Anh ta không bị chấn thương trước đó hoặc đau tinh hoàn cấp tính. Cắt tinh hoàn một phần được thực hiện thông qua một vết rạch bẹn với việc cắt bỏ các tổn thương và tái tạo nhu mô tinh hoàn. Kiểm tra mô học cho thấy nhồi máu tinh hoàn không có sự hiện diện của các tế bào tân sinh."} +{"idx": 1990109, "en": "Increasing evidence suggests that epigenetic factors have critical roles in gene regulation in neuropsychiatric disorders and in aging, both of which are typically associated with a wide range of gene expression abnormalities. Here, we have used chromatin immunoprecipitation-qPCR to measure levels of acetylated histone H3 at lysines 9/14 (ac-H3K9K14), two epigenetic marks associated with transcriptionally active chromatin, at the promoter regions of eight schizophrenia-related genes in n=82 postmortem prefrontal cortical samples from normal subjects and those with schizophrenia and bipolar disorder. We find that promoter-associated ac-H3K9K14 levels are correlated with gene expression levels, as measured by real-time qPCR for several genes, including, glutamic acid decarboxylase 1 (GAD1), 5-hydroxytryptamine receptor 2C (HTR2C), translocase of outer mitochondrial membrane 70 homolog A (TOMM70A) and protein phosphatase 1E (PPM1E). Ac-H3K9K14 levels of several of the genes tested were significantly negatively associated with age in normal subjects and those with bipolar disorder, but not in subjects with schizophrenia, whereby low levels of histone acetylation were observed in early age and throughout aging. Consistent with this observation, significant hypoacetylation of H3K9K14 was detected in young subjects with schizophrenia when compared with age-matched controls. Our results demonstrate that gene expression changes associated with psychiatric disease and aging result from epigenetic mechanisms involving histone acetylation. We further find that treatment with a histone deacetylase (HDAC) inhibitor alters the expression of several candidate genes for schizophrenia in mouse brain. These findings may have therapeutic implications for the clinical use of HDAC inhibitors in psychiatric disorders.", "gemini": "Ngày càng có nhiều bằng chứng cho thấy các yếu tố di truyền có vai trò quan trọng trong quá trình điều hòa gen ở các rối loạn tâm thần kinh và trong quá trình lão hóa, cả hai đều thường liên quan đến nhiều bất thường về biểu hiện gen. Ở đây, chúng tôi đã sử dụng kết tủa miễn dịch chromatin-qPCR để đo mức độ histone H3 acetylated ở lysine 9/14 (ac-H3K9K14), hai dấu hiệu di truyền liên quan đến chromatin hoạt động phiên mã, tại các vùng khởi động của tám gen liên quan đến tâm thần phân liệt trong n=82 mẫu vỏ não trước trán sau tử thi từ những đối tượng bình thường và những người mắc bệnh tâm thần phân liệt và rối loạn lưỡng cực. Chúng tôi thấy rằng mức độ ac-H3K9K14 liên quan đến vùng khởi động có tương quan với mức độ biểu hiện gen, được đo bằng qPCR thời gian thực đối với một số gen, bao gồm, glutamate decarboxylase 1 (GAD1), thụ thể 5-hydroxytryptamine 2C (HTR2C), translocase của màng ty thể ngoài 70 đồng dạng A (TOMM70A) và protein phosphatase 1E (PPM1E). Mức độ Ac-H3K9K14 của một số gen được thử nghiệm có liên quan đáng kể đến tuổi ở những người bình thường và những người mắc chứng rối loạn lưỡng cực, nhưng không phải ở những người mắc bệnh tâm thần phân liệt, theo đó mức độ acetylation histone thấp được quan sát thấy ở độ tuổi sớm và trong suốt quá trình lão hóa. Phù hợp với quan sát này, tình trạng giảm acetylation đáng kể của H3K9K14 đã được phát hiện ở những đối tượng trẻ mắc bệnh tâm thần phân liệt khi so sánh với những người đối chứng có độ tuổi phù hợp. Kết quả của chúng tôi chứng minh rằng những thay đổi biểu hiện gen liên quan đến bệnh tâm thần và lão hóa là kết quả của các cơ chế di truyền liên quan đến quá trình acetylation histone. Hơn nữa, chúng tôi thấy rằng việc điều trị bằng chất ức chế histone deacetylase (HDAC) làm thay đổi biểu hiện của một số gen ứng viên cho bệnh tâm thần phân liệt ở não chuột. Những phát hiện này có thể có ý nghĩa điều trị đối với việc sử dụng lâm sàng chất ức chế HDAC trong các rối loạn tâm thần."} +{"idx": 1990110, "en": "Neuromedin S (NMS), a peptide structurally related to NMU, has been identified in the mammalian heart tissues. However to date, any role of NMS in cardiomyocytes and the relevant mechanisms still remain unknown. In this study, we identified a novel functional role of NMS in modulating L-type Ca(2+) channels in adult rat ventricular myocytes, in which NMU type 2 receptors (NMUR2), but not NMUR1, are endogenously expressed. We found that NMS at 0.1 µM reversibly increased I(Ba) by ~29.7%. Intracellular infusion of GDP-β-S or a selective antibody raised against the G(i)-protein blocked the stimulatory effects of NMS. The classical and novel protein kinase C (nPKC) antagonist calphostin C or chelerythrine chloride, as well as the phospholipase C (PLC) inhibitor U73122, abolished NMS responses, whereas a classical PKC antagonist Gö6976 or a PKA antagonist PKI 5-24 had no such effects. Pretreatment of cells with PKC-δ specific inhibitor rottlerin or intracellular application of a PKC-δ-derived inhibitory peptide, δV1-1, abolished NMS responses, while an inactive control peptide had no effects. In summary, NMS acting through NMUR2 increases I(Ba) via a G(i)α-protein-dependent PKC-δ pathway in rat ventricular myocytes.", "gemini": "Neuromedin S (NMS), một peptide có cấu trúc liên quan đến NMU, đã được xác định trong các mô tim của động vật có vú. Tuy nhiên, cho đến nay, bất kỳ vai trò nào của NMS trong tế bào cơ tim và các cơ chế liên quan vẫn chưa được biết. Trong nghiên cứu này, chúng tôi đã xác định một vai trò chức năng mới của NMS trong việc điều hòa các kênh Ca(2+) loại L ở tế bào cơ tim thất của chuột trưởng thành, trong đó thụ thể NMU loại 2 (NMUR2), nhưng không phải NMUR1, được biểu hiện nội sinh. Chúng tôi thấy rằng NMS ở 0,1 µM làm tăng I(Ba) một cách thuận nghịch khoảng 29,7%. Truyền nội bào GDP-β-S hoặc một kháng thể chọn lọc chống lại protein G(i) đã ngăn chặn các tác dụng kích thích của NMS. Chất đối kháng protein kinase C (nPKC) cổ điển và mới calphostin C hoặc chelerythrine chloride, cũng như chất ức chế phospholipase C (PLC) U73122, đã xóa bỏ các phản ứng NMS, trong khi chất đối kháng PKC cổ điển Gö6976 hoặc chất đối kháng PKA PKI 5-24 không có tác dụng như vậy. Xử lý trước các tế bào bằng chất ức chế đặc hiệu PKC-δ rottlerin hoặc ứng dụng nội bào của một peptide ức chế có nguồn gốc từ PKC-δ, δV1-1, đã xóa bỏ các phản ứng NMS, trong khi một peptide đối chứng không hoạt động không có tác dụng. Tóm lại, NMS hoạt động thông qua NMUR2 làm tăng I(Ba) thông qua con đường PKC-δ phụ thuộc protein G(i)α trong tế bào cơ tim thất của chuột."} +{"idx": 1990111, "en": "BACKGROUND Although endobronchial ultrasound (EBUS)-guided transbronchial biopsy (TBB) has been shown to increase the diagnostic yield over conventional bronchoscopic techniques, an important issue regarding the optimal number of biopsy specimens required has not been thoroughly investigated. OBJECTIVES We sought to examine whether the number of biopsy specimens taken was associated with the diagnostic yield of EBUS-guided TBB and, if this was the case, to determine the optimal number of specimens required for the maximum diagnostic yield in peripheral pulmonary lesions. METHODS The medical records of patients undergoing EBUS-guided TBB for the diagnosis of peripheral pulmonary lesions from 2008 to 2010 were retrospectively reviewed. The association of clinical and radiological features, including the number of biopsy specimens, with the diagnostic yield was analysed. RESULTS A total of 384 patients were included for analysis. The overall diagnostic yield of EBUS-guided TBB was 73%, and the only factor influencing the diagnostic yield was the position of the probe. Patients in which the EBUS probe was placed within the lesions had a significantly higher yield (85%) than those in which the probe was adjacent to or outside the lesions (38%; p < 0.001). When the number of biopsy specimens was determined based on their adequacy, it was an insignificant factor in predicting the diagnostic yield. CONCLUSIONS Probe position independently predicts the diagnostic yield of EBUS-guided TBB. In real-world practice, the optimal number of biopsy specimens should be decided on a case-by-case basis.", "gemini": "LÝ LỊCH Mặc dù sinh thiết xuyên phế quản (TBB) có hướng dẫn siêu âm nội phế quản (EBUS) đã được chứng minh là làm tăng hiệu suất chẩn đoán so với các kỹ thuật soi phế quản thông thường, nhưng một vấn đề quan trọng liên quan đến số lượng mẫu sinh thiết tối ưu cần thiết vẫn chưa được nghiên cứu kỹ lưỡng. MỤC TIÊU Chúng tôi tìm cách kiểm tra xem số lượng mẫu sinh thiết có liên quan đến hiệu suất chẩn đoán của TBB có hướng dẫn EBUS hay không và nếu đúng như vậy, hãy xác định số lượng mẫu tối ưu cần thiết để đạt được hiệu suất chẩn đoán tối đa đối với các tổn thương phổi ngoại vi. PHƯƠNG PHÁP Các hồ sơ bệnh án của những bệnh nhân trải qua TBB có hướng dẫn EBUS để chẩn đoán các tổn thương phổi ngoại vi từ năm 2008 đến năm 2010 đã được xem xét lại một cách hồi cứu. Sự liên quan của các đặc điểm lâm sàng và hình ảnh, bao gồm số lượng mẫu sinh thiết, với hiệu suất chẩn đoán đã được phân tích. KẾT QUẢ Tổng cộng có 384 bệnh nhân được đưa vào phân tích. Hiệu suất chẩn đoán chung của TBB có hướng dẫn EBUS là 73% và yếu tố duy nhất ảnh hưởng đến hiệu suất chẩn đoán là vị trí của đầu dò. Những bệnh nhân có đầu dò EBUS được đặt trong các tổn thương có hiệu suất cao hơn đáng kể (85%) so với những bệnh nhân có đầu dò nằm cạnh hoặc bên ngoài các tổn thương (38%; p < 0,001). Khi số lượng mẫu sinh thiết được xác định dựa trên tính đầy đủ của chúng, thì đó là một yếu tố không đáng kể trong việc dự đoán hiệu suất chẩn đoán. KẾT LUẬN Vị trí đầu dò dự đoán độc lập hiệu suất chẩn đoán của TBB có hướng dẫn EBUS. Trong thực tế, số lượng mẫu sinh thiết tối ưu nên được quyết định theo từng trường hợp cụ thể."} +{"idx": 1990112, "en": "Globally, more than 1000 tonnes of titanium (Ti) is implanted into patients in the form of biomedical devices on an annual basis. Ti is perceived to be 'biocompatible' owing to the presence of a robust passive oxide film (approx. 4 nm thick) at the metal surface. However, surface deterioration can lead to the release of Ti ions, and particles can arise as the result of wear and/or corrosion processes. This surface deterioration can result in peri-implant inflammation, leading to the premature loss of the implanted device or the requirement for surgical revision. Soft tissues surrounding commercially pure cranial anchorage devices (bone-anchored hearing aid) were investigated using synchrotron X-ray micro-fluorescence spectroscopy and X-ray absorption near edge structure. Here, we present the first experimental evidence that minimal load-bearing Ti implants, which are not subjected to macroscopic wear processes, can release Ti debris into the surrounding soft tissue. As such debris has been shown to be pro-inflammatory, we propose that such distributions of Ti are likely to effect to the service life of the device.", "gemini": "Trên toàn cầu, hơn 1000 tấn titan (Ti) được cấy ghép vào bệnh nhân dưới dạng thiết bị y sinh hàng năm. Ti được coi là 'tương thích sinh học' do sự hiện diện của một lớp oxit thụ động mạnh mẽ (dày khoảng 4 nm) trên bề mặt kim loại. Tuy nhiên, sự suy giảm bề mặt có thể dẫn đến giải phóng các ion Ti và các hạt có thể phát sinh do quá trình mài mòn và/hoặc ăn mòn. Sự suy giảm bề mặt này có thể dẫn đến tình trạng viêm quanh implant, dẫn đến mất sớm thiết bị cấy ghép hoặc yêu cầu phẫu thuật sửa đổi. Các mô mềm xung quanh các thiết bị neo chèn sọ tinh khiết thương mại (máy trợ thính neo xương) đã được nghiên cứu bằng phương pháp quang phổ huỳnh quang vi tia X đồng bộ và cấu trúc hấp thụ tia X gần cạnh. Tại đây, chúng tôi trình bày bằng chứng thực nghiệm đầu tiên cho thấy các implant Ti chịu tải tối thiểu, không phải chịu các quá trình mài mòn vĩ mô, có thể giải phóng các mảnh vụn Ti vào mô mềm xung quanh. Vì các mảnh vụn như vậy đã được chứng minh là gây viêm, chúng tôi đề xuất rằng sự phân bố Ti như vậy có khả năng ảnh hưởng đến tuổi thọ của thiết bị."} +{"idx": 1990113, "en": "Patterns of species interactions affect the dynamics of food webs. An important component of species interactions that is rarely considered with respect to food webs is the strengths of interactions, which may affect both structure and dynamics. In natural systems, these strengths are variable, and can be quantified as probability distributions. We examined how variation in strengths of interactions can be described hierarchically, and how this variation impacts the structure of species interactions in predator-prey networks, both of which are important components of ecological food webs. The stable isotope ratios of predator and prey species may be particularly useful for quantifying this variability, and we show how these data can be used to build probabilistic predator-prey networks. Moreover, the distribution of variation in strengths among interactions can be estimated from a limited number of observations. This distribution informs network structure, especially the key role of dietary specialization, which may be useful for predicting structural properties in systems that are difficult to observe. Finally, using three mammalian predator-prey networks (two African and one Canadian) quantified from stable isotope data, we show that exclusion of link-strength variability results in biased estimates of nestedness and modularity within food webs, whereas the inclusion of body size constraints only marginally increases the predictive accuracy of the isotope-based network. We find that modularity is the consequence of strong link-strengths in both African systems, while nestedness is not significantly present in any of the three predator-prey networks.", "gemini": "Các mô hình tương tác giữa các loài ảnh hưởng đến động lực của lưới thức ăn. Một thành phần quan trọng của các tương tác giữa các loài hiếm khi được xem xét liên quan đến lưới thức ăn là cường độ tương tác, có thể ảnh hưởng đến cả cấu trúc và động lực. Trong các hệ thống tự nhiên, những cường độ này là biến đổi và có thể được định lượng thành phân phối xác suất. Chúng tôi đã kiểm tra cách biến đổi cường độ tương tác có thể được mô tả theo hệ thống phân cấp và cách biến đổi này tác động đến cấu trúc của các tương tác giữa các loài trong mạng lưới con mồi-động vật ăn thịt, cả hai đều là thành phần quan trọng của lưới thức ăn sinh thái. Tỷ lệ đồng vị ổn định của các loài động vật ăn thịt và con mồi có thể đặc biệt hữu ích để định lượng sự biến đổi này và chúng tôi chỉ ra cách các dữ liệu này có thể được sử dụng để xây dựng các mạng lưới con mồi-động vật ăn thịt theo xác suất. Hơn nữa, có thể ước tính sự phân bố của biến đổi cường độ giữa các tương tác từ một số lượng giới hạn các quan sát. Sự phân bố này thông báo cấu trúc mạng, đặc biệt là vai trò chính của sự chuyên biệt về chế độ ăn, có thể hữu ích để dự đoán các đặc tính cấu trúc trong các hệ thống khó quan sát. Cuối cùng, sử dụng ba mạng lưới con mồi-động vật ăn thịt ở động vật có vú (hai ở Châu Phi và một ở Canada) được định lượng từ dữ liệu đồng vị ổn định, chúng tôi chỉ ra rằng việc loại trừ tính biến đổi cường độ liên kết dẫn đến ước tính sai về tính lồng nhau và tính mô đun trong lưới thức ăn, trong khi việc đưa vào các ràng buộc về kích thước cơ thể chỉ làm tăng biên độ dự đoán của mạng dựa trên đồng vị một cách không đáng kể. Chúng tôi thấy rằng tính mô đun là hậu quả của cường độ liên kết mạnh trong cả hai hệ thống ở Châu Phi, trong khi tính lồng nhau không có mặt đáng kể trong bất kỳ mạng lưới con mồi-động vật ăn thịt nào trong ba mạng lưới."} +{"idx": 1990114, "en": "Light absorption by carotenoids is known to vary substantially with the shape or conformation of the pigment molecule induced by the molecular environment, but the role of interactions between carotenoid pigments and the proteins to which they are bound, and the resulting impact on organismal coloration, remain unclear. Here, we present a spectroscopic investigation of feathers from the brilliant red scarlet ibis (Eudocimus ruber, Threskiornithidae), the orange-red summer tanager (Piranga rubra, Cardinalidae) and the violet-purple feathers of the white-browed purpletuft (Iodopleura isabellae, Tityridae). Despite their striking differences in colour, all three of these feathers contain canthaxanthin (β,β-carotene-4,4'-dione) as their primary pigment. Reflectance and resonance Raman (rR) spectroscopy were used to investigate the induced molecular structural changes and carotenoid-protein interactions responsible for the different coloration in these plumage samples. The results demonstrate a significant variation between species in the peak frequency of the strong ethylenic vibration (ν(1)) peak in the rR spectra, the most significant of which is found in I. isabellae feathers and is correlated with a red-shift in canthaxanthin absorption that results in violet reflectance. Neither polarizability of the protein environment nor planarization of the molecule upon binding can entirely account for the full extent of the colour shift. Therefore, we suggest that head-to-tail molecular alignment (i.e. J-aggregation) of the protein-bound carotenoid molecules is an additional factor.", "gemini": "Sự hấp thụ ánh sáng của carotenoid được biết là thay đổi đáng kể tùy theo hình dạng hoặc cấu hình của phân tử sắc tố do môi trường phân tử gây ra, nhưng vai trò của các tương tác giữa sắc tố carotenoid và các protein mà chúng liên kết, và tác động kết quả lên màu sắc của sinh vật, vẫn chưa rõ ràng. Ở đây, chúng tôi trình bày một nghiên cứu quang phổ về lông vũ từ loài ibis đỏ tươi rực rỡ (Eudocimus ruber, Threskiornithidae), loài tanager mùa hè màu cam đỏ (Piranga rubra, Cardinalidae) và lông vũ màu tím của loài chim đầu tím lông mày trắng (Iodopleura isabellae, Tityridae). Mặc dù có sự khác biệt rõ rệt về màu sắc, cả ba loại lông vũ này đều chứa canthaxanthin (β,β-caroten-4,4'-dione) là sắc tố chính của chúng. Phản xạ và quang phổ cộng hưởng Raman (rR) đã được sử dụng để nghiên cứu các thay đổi cấu trúc phân tử được tạo ra và các tương tác carotenoid-protein chịu trách nhiệm cho sự khác biệt về màu sắc trong các mẫu lông vũ này. Kết quả chứng minh sự thay đổi đáng kể giữa các loài về tần số đỉnh của đỉnh rung ethylen mạnh (ν(1)) trong quang phổ rR, trong đó đáng kể nhất được tìm thấy ở lông vũ I. isabellae và có tương quan với sự dịch chuyển đỏ trong hấp thụ canthaxanthin dẫn đến phản xạ màu tím. Cả độ phân cực của môi trường protein cũng như sự phẳng của phân tử khi liên kết đều không thể giải thích hoàn toàn về mức độ dịch chuyển màu. Do đó, chúng tôi cho rằng sự liên kết phân tử đầu đuôi (tức là sự kết hợp J) của các phân tử carotenoid liên kết protein là một yếu tố bổ sung."} +{"idx": 1990115, "en": "A number of biological processes, such as embryo development, cancer metastasis or wound healing, rely on cells moving in concert. The mechanisms leading to the emergence of coordinated motion remain however largely unexplored. Although biomolecular signalling is known to be involved in most occurrences of collective migration, the role of physical and mechanical interactions has only been recently investigated. In this study, a versatile framework for cell motility is implemented in silico in order to study the minimal requirements for the coordination of a group of epithelial cells. We find that cell motility and cell-cell mechanical interactions are sufficient to generate a broad array of behaviours commonly observed in vitro and in vivo. Cell streaming, sheet migration and susceptibility to leader cells are examples of behaviours spontaneously emerging from these simple assumptions, which might explain why collective effects are so ubiquitous in nature. The size of the population and its confinement appear, in particular, to play an important role in the coordination process. In all cases, the complex response of the population can be predicted from the knowledge of the correlation length of the velocity field measured in the bulk of the epithelial layer. This analysis provides also new insights into cancer metastasis and cell sorting, suggesting, in particular, that collective invasion might result from an emerging coordination in a system where single cells are mechanically unable to invade.", "gemini": "Một số quá trình sinh học, chẳng hạn như phát triển phôi, di căn ung thư hoặc chữa lành vết thương, phụ thuộc vào sự di chuyển của các tế bào theo hòa hợp. Tuy nhiên, các cơ chế dẫn đến sự xuất hiện của chuyển động phối hợp vẫn chưa được khám phá nhiều. Mặc dù tín hiệu sinh học được biết là có liên quan đến hầu hết các trường hợp di chuyển tập thể, nhưng vai trò của các tương tác vật lý và cơ học chỉ mới được nghiên cứu gần đây. Trong nghiên cứu này, một khuôn khổ linh hoạt cho khả năng vận động của tế bào được triển khai trong silico để nghiên cứu các yêu cầu tối thiểu để phối hợp một nhóm các tế bào biểu mô. Chúng tôi thấy rằng khả năng vận động của tế bào và các tương tác cơ học tế bào-tế bào là đủ để tạo ra một loạt các hành vi thường được quan sát thấy trong ống nghiệm và trong cơ thể sống. Dòng tế bào, di chuyển theo tấm và khả năng tiếp nhận các tế bào dẫn đầu là những ví dụ về hành vi tự phát phát sinh từ những giả định đơn giản này, có thể giải thích tại sao các hiệu ứng tập thể lại phổ biến trong tự nhiên như vậy. Đặc biệt, kích thước của quần thể và sự giới hạn của nó đóng một vai trò quan trọng trong quá trình phối hợp. Trong mọi trường hợp, phản ứng phức tạp của quần thể có thể được dự đoán từ kiến thức về độ dài tương quan của trường vận tốc được đo trong phần lớn lớp biểu mô. Phân tích này cũng cung cấp những hiểu biết mới về di căn ung thư và phân loại tế bào, đặc biệt cho thấy rằng sự xâm lấn tập thể có thể là kết quả của sự phối hợp mới nổi trong một hệ thống mà các tế bào đơn lẻ không có khả năng xâm lấn về mặt cơ học."} +{"idx": 1990116, "en": "By determining the most common English words and phrases since the beginning of the sixteenth century, we obtain a unique large-scale view of the evolution of written text. We find that the most common words and phrases in any given year had a much shorter popularity lifespan in the sixteenth century than they had in the twentieth century. By measuring how their usage propagated across the years, we show that for the past two centuries, the process has been governed by linear preferential attachment. Along with the steady growth of the English lexicon, this provides an empirical explanation for the ubiquity of Zipf's law in language statistics and confirms that writing, although undoubtedly an expression of art and skill, is not immune to the same influences of self-organization that are known to regulate processes as diverse as the making of new friends and World Wide Web growth.", "gemini": "Bằng cách xác định các từ và cụm từ tiếng Anh phổ biến nhất kể từ đầu thế kỷ XVI, chúng tôi có được một cái nhìn toàn cảnh độc đáo về sự tiến hóa của văn bản viết. Chúng tôi thấy rằng các từ và cụm từ phổ biến nhất trong bất kỳ năm nào đều có tuổi thọ phổ biến ngắn hơn nhiều vào thế kỷ XVI so với thế kỷ XX. Bằng cách đo cách sử dụng của chúng lan truyền qua nhiều năm, chúng tôi chỉ ra rằng trong hai thế kỷ qua, quá trình này đã được điều chỉnh bởi sự gắn kết ưu tiên tuyến tính. Cùng với sự phát triển ổn định của từ vựng tiếng Anh, điều này cung cấp một lời giải thích thực nghiệm cho tính phổ biến của định luật Zipf trong thống kê ngôn ngữ và xác nhận rằng văn bản, mặc dù chắc chắn là một biểu hiện của nghệ thuật và kỹ năng, nhưng không miễn nhiễm với những ảnh hưởng của sự tự tổ chức được biết là điều chỉnh các quá trình đa dạng như việc kết bạn mới và sự phát triển của World Wide Web."} +{"idx": 1990117, "en": "BACKGROUND In clinical randomized controlled trials (RCTs), decompressive surgery (DS) for malignant middle cerebral artery (MMCA) infarcts leads to a 50% absolute risk reduction in mortality, and improves the 1-year functional outcome. The reproducibility of these results in routine practice has never been evaluated. The purpose of this study was to test the hypothesis that the results of DS for MMCA in practice are similar to those observed in the surgical group of RCTs. METHODS We prospectively included the first 31 patients who underwent DS for MMCA. They were screened based on similar criteria as in the meta-analysis. The primary outcome was a modified Rankin Scale (mRS) score of ≤4, and secondary outcomes were mRS of ≤3 and death at 1 year. RESULTS Thirty-one patients underwent DS for MMCA. The 1-year mRS was ≤4 in 22 patients (71.0%) and ≤3 in 16 (51.6%). Seven patients died (22.6%). CONCLUSION This observational study showed that DS for MMCA in a center without previous experience provides similar results as those obtained in the surgical arm of RCTs.", "gemini": "BỐI CẢNH Trong các thử nghiệm lâm sàng ngẫu nhiên có đối chứng (RCT), phẫu thuật giảm áp (DS) đối với nhồi máu động mạch não giữa ác tính (MMCA) dẫn đến giảm 50% nguy cơ tử vong tuyệt đối và cải thiện kết quả chức năng sau 1 năm. Tính khả thi của những kết quả này trong thực hành thường quy chưa bao giờ được đánh giá. Mục đích của nghiên cứu này là kiểm tra giả thuyết rằng kết quả của DS đối với MMCA trong thực hành tương tự như những kết quả quan sát được ở nhóm phẫu thuật của RCT. PHƯƠNG PHÁP Chúng tôi đã đưa vào nghiên cứu theo dõi 31 bệnh nhân đầu tiên đã trải qua DS đối với MMCA. Họ được sàng lọc dựa trên các tiêu chí tương tự như trong phân tích tổng hợp. Kết quả chính là điểm Thang Rankin đã sửa đổi (mRS) ≤4 và các kết quả phụ là mRS ≤3 và tử vong sau 1 năm. KẾT QUẢ Ba mươi mốt bệnh nhân đã trải qua DS đối với MMCA. mRS sau 1 năm là ≤4 ở 22 bệnh nhân (71,0%) và ≤3 ở 16 bệnh nhân (51,6%). Bảy bệnh nhân đã tử vong (22,6%). KẾT LUẬN Nghiên cứu quan sát này cho thấy DS đối với MMCA tại một trung tâm chưa có kinh nghiệm trước đó mang lại kết quả tương tự như kết quả thu được ở nhóm phẫu thuật của RCT."} +{"idx": 1990118, "en": "Clinicians routinely assess scapular position and motion of the symptomatic shoulder taking as reference for the contralateral asymptomatic side. A different positioning between sides (scapular asymmetry) is often assumed as pathological, however, the symmetry of scapular kinematics in healthy individuals is yet to be demonstrated. This study tested the hypothesis of scapular symmetry during arm elevation. The 3-dimensional scapular positioning of the dominant and non-dominant shoulders of fourteen healthy young adults was simultaneously measured by a 6 degrees of freedom electromagnetic tracking device at three positions of arm elevation: rest, hands on hips, and 90° of shoulder abduction with internal rotation. The scapula on the dominant shoulder showed greater retraction (P < 0.001; η(2)(p) = 0.68) and upward rotation (P < 0.001; η(2)(p) = 0.70) at all positions of arm elevation. From rest to 90° of shoulder abduction, the mean (±SD) amount of scapular angular displacement was, respectively for dominant and non-dominant shoulders, 7.2° (±7.8°) and 7.2° (±4.4°) for retraction, 17.4° (±5.1°) and 17.8° (±6.4°) for upward rotation, and 3.8° (±3.6°) and 0.9° (±3.6°) for posterior tilting. These findings suggest that scapular positioning on the thorax are not the same despite the observation of an identical kinematic pattern during arm elevation. This should be taken into consideration when comparing scapular position and motion of symptomatic and contralateral shoulders.", "gemini": "Các bác sĩ lâm sàng thường đánh giá vị trí xương vai và chuyển động của vai có triệu chứng, lấy vai bên đối diện không có triệu chứng làm tham chiếu. Một vị trí khác nhau giữa các bên (vai không cân xứng) thường được coi là bệnh lý, tuy nhiên, tính đối xứng của động học xương vai ở những cá thể khỏe mạnh vẫn chưa được chứng minh. Nghiên cứu này đã kiểm tra giả thuyết về tính đối xứng của xương vai trong quá trình nâng cánh tay. Vị trí xương vai 3 chiều của vai thuận và vai không thuận của mười bốn người trẻ khỏe đã được đo đồng thời bằng thiết bị theo dõi điện từ 6 độ tự do ở ba vị trí nâng cánh tay: nghỉ, chống tay vào hông và 90° cánh tay dạng ra ngoài với xoay trong. Xương vai ở vai thuận cho thấy độ thụt vào lớn hơn (P < 0,001; η(2)(p) = 0,68) và độ xoay lên lớn hơn (P < 0,001; η(2)(p) = 0,70) ở mọi vị trí nâng cánh tay. Từ trạng thái nghỉ đến 90° cánh tay dạng ra ngoài, độ dịch chuyển góc xương vai trung bình (±SD) lần lượt là đối với vai thuận và vai không thuận, 7,2° (±7,8°) và 7,2° (±4,4°) đối với độ thụt vào, 17,4° (±5,1°) và 17,8° (±6,4°) đối với độ xoay lên, và 3,8° (±3,6°) và 0,9° (±3,6°) đối với độ nghiêng sau. Những phát hiện này cho thấy rằng vị trí xương vai trên ngực không giống nhau mặc dù quan sát thấy một kiểu động học giống hệt nhau trong quá trình nâng cánh tay. Điều này cần được cân nhắc khi so sánh vị trí và chuyển động của xương vai giữa vai có triệu chứng và vai đối bên."} +{"idx": 1990119, "en": "Protozoan parasites cause severe morbidity and mortality in humans worldwide, especially in developing countries where access to chemotherapeutic agents is limited. Although parasites initially evoke a robust immune response, subsequent immunity fails to clear infection, ultimately leading to the chronic stage. This enigmatic situation was initially addressed in chronic viral models, where T cells lose their function, a phenomenon referred to as 'exhaustion'. However, recent studies demonstrate that this paradigm can be extended to protozoan diseases as well, although with notable differences. These studies have revealed that T cell responses generated against Toxoplasma gondii, Plasmodium sp., and Leishmania sp. can become dysfunctional. This review discusses T cell exhaustion in parasitic infection, mechanisms of development, and a possible role in disease outcome.", "gemini": "Ký sinh trùng nguyên sinh gây bệnh tật và tử vong nghiêm trọng ở người trên toàn thế giới, đặc biệt là ở các nước đang phát triển nơi khả năng tiếp cận các tác nhân hóa trị liệu bị hạn chế. Mặc dù ban đầu ký sinh trùng gây ra phản ứng miễn dịch mạnh mẽ, nhưng khả năng miễn dịch sau đó không thể loại trừ nhiễm trùng, cuối cùng dẫn đến giai đoạn mãn tính. Tình trạng khó hiểu này ban đầu được giải quyết trong các mô hình vi-rút mãn tính, trong đó tế bào T mất chức năng, một hiện tượng được gọi là 'kiệt sức'. Tuy nhiên, các nghiên cứu gần đây chứng minh rằng mô hình này cũng có thể mở rộng sang các bệnh do ký sinh trùng nguyên sinh, mặc dù có những điểm khác biệt đáng chú ý. Các nghiên cứu này đã tiết lộ rằng các phản ứng tế bào T được tạo ra chống lại Toxoplasma gondii, Plasmodium sp. và Leishmania sp. có thể trở nên rối loạn chức năng. Bài tổng quan này thảo luận về tình trạng kiệt sức của tế bào T trong nhiễm trùng ký sinh trùng, cơ chế phát triển và vai trò có thể có trong kết cục của bệnh."} +{"idx": 1990120, "en": "In domestic pigs strict control measures and the use of gene-deleted marker vaccines resulted in the elimination of pseudorabies virus (PrV) infections in many parts of Europe and North America. In free-roaming feral pigs and wild boar populations, however, serological surveys and monitoring in The Americas, Europe and North Africa provided serological and virological evidence that PrV is more widely distributed than previously assumed. Thus, there is a constant risk of spillover of PrV infection from wild pig populations to domestic animals which could require intervention to limit the infection in wild pigs. To investigate whether oral immunization of wild boar by live-attenuated PrV could be an option, wild boar and domestic pigs were orally immunized with 2×10(6) TCID(50) of the attenuated live PrV vaccine strain Bartha supplied either with a syringe or within a blister, and subsequently intranasally challenged with 10(6) TCID(50) of the highly virulent PrV strain NIA-3. Oral immunization with live-attenuated PrV was able to confer protection against clinical signs in wild boar and against transmission of challenge virus to naïve contact animals. Only two vaccinated domestic pigs developed neurological signs after challenge infection. Our results demonstrate that oral immunization against PrV infection in wild boar is possible. In case increasing PrV infection rates in wild boar may enhance the risk for spillover into domestic pig populations, oral immunization of wild boar against PrV in endemic areas might be a feasible control strategy.", "gemini": "Ở những con lợn trong nước, các biện pháp kiểm soát nghiêm ngặt và việc sử dụng vắc-xin đánh dấu gen đã xóa được loại trừ các bệnh nhiễm vi-rút giả dại (PrV) ở nhiều khu vực của Châu Âu và Bắc Mỹ. Tuy nhiên, ở những đàn lợn hoang và lợn rừng chạy rông, các cuộc khảo sát huyết thanh và giám sát ở Châu Mỹ, Châu Âu và Bắc Phi đã cung cấp bằng chứng huyết thanh và vi-rút học cho thấy PrV phân bố rộng hơn so với trước đây. Do đó, luôn có nguy cơ lây lan bệnh nhiễm PrV từ các đàn lợn hoang sang động vật nuôi trong nước, có thể cần can thiệp để hạn chế sự lây nhiễm ở lợn hoang. Để tìm hiểu xem liệu việc tiêm chủng bằng vắc-xin PrV sống giảm độc lực qua đường miệng cho lợn rừng có thể là một lựa chọn hay không, lợn rừng và lợn trong nước đã được tiêm chủng bằng vắc-xin PrV sống giảm độc lực Bartha với liều 2 × 10 (6) TCID (50) bằng ống tiêm hoặc trong vỉ thuốc, và sau đó thử nghiệm bằng cách nhỏ mũi 10 (6) TCID (50) của chủng PrV độc lực cao NIA-3. Tiêm chủng bằng vắc-xin PrV sống giảm độc lực qua đường miệng có thể bảo vệ lợn rừng khỏi các dấu hiệu lâm sàng và chống lại sự lây truyền vi-rút thử thách sang các động vật tiếp xúc chưa được tiêm vắc-xin. Chỉ có hai con lợn trong nước được tiêm vắc-xin phát triển các dấu hiệu thần kinh sau khi thử thách nhiễm trùng. Kết quả của chúng tôi chứng minh rằng việc tiêm chủng bằng vắc-xin qua đường miệng để chống lại bệnh nhiễm PrV ở lợn rừng là khả thi. Trong trường hợp tỷ lệ nhiễm PrV ngày càng tăng ở lợn rừng có thể làm tăng nguy cơ lây lan sang đàn lợn trong nước, thì việc tiêm chủng bằng vắc-xin qua đường miệng cho lợn rừng chống lại PrV ở các khu vực lưu hành có thể là một chiến lược kiểm soát khả thi."} +{"idx": 1990121, "en": "OBJECTIVES This study investigated white matter alterations in the corpus callosum (CC) and centrum semiovale (CSO), using diffusion tensor imaging and magnetization transfer imaging, in participants with severe traumatic brain injury (TBI) and related these changes to processing speed measures. PARTICIPANTS AND METHODS Fourteen adult participants with severe TBI underwent neuroimaging and assessment, using the Symbol Digit Modalities Test and Trail-Making Test, Part B, at approximately 6 months postinjury. Thirteen demographically similar, neurologically intact adults were imaged for comparison. RESULTS The TBI group demonstrated lower fractional anisotropy (FA) for the right CSO and higher apparent diffusion coefficient (ADC) for the CSO bilaterally than the control group. Lower FA and higher ADC were noted in all CC regions. Magnetization transfer imaging revealed smaller magnetization transfer ratios (MTRs) in the right and left CSO and CC genu and splenium. Written Symbol Digit Modalities Test performance was related to right CSO FA, bilateral CSO ADC, CC FA, and right CSO MTR, whereas oral Symbol Digit Modalities Test was related to right CSO FA, ADC, and MTR. Trail-Making Test, Part B, was related to right CSO FA and MTR. CONCLUSIONS Advanced neuroimaging modalities such as diffusion tensor imaging and magnetization transfer imaging demonstrate significant alterations in white matter, which are related to processing speed. These techniques may be useful in quantifying the extent of injury even in normal appearing white matter after TBI.", "gemini": "MỤC TIÊU Nghiên cứu này đã điều tra những thay đổi về chất trắng ở thể chai (CC) và chất trắng trung tâm (CSO), sử dụng phương pháp chụp ảnh tensor khuếch tán và chụp ảnh truyền từ, ở những người tham gia bị chấn thương sọ não nghiêm trọng (TBI) và liên hệ những thay đổi này với các biện pháp về tốc độ xử lý. NGƯỜI THAM GIA VÀ PHƯƠNG PHÁP Mười bốn người tham gia trưởng thành bị TBI nghiêm trọng đã được chụp ảnh thần kinh và đánh giá, sử dụng Bài kiểm tra các phương thức chữ số biểu tượng và Bài kiểm tra vẽ đường mòn, Phần B, vào khoảng 6 tháng sau chấn thương. Mười ba người lớn khỏe mạnh về thần kinh, có thông tin nhân khẩu học tương tự đã được chụp ảnh để so sánh. KẾT QUẢ Nhóm TBI có độ dị hướng phân đoạn (FA) thấp hơn đối với CSO bên phải và hệ số khuếch tán biểu kiến (ADC) cao hơn đối với CSO ở cả hai bên so với nhóm đối chứng. FA thấp hơn và ADC cao hơn được ghi nhận ở tất cả các vùng CC. Chụp ảnh truyền từ cho thấy tỷ lệ truyền từ (MTR) nhỏ hơn ở CSO bên phải và bên trái và đầu gối và thân của CC. Hiệu suất của Bài kiểm tra các phương thức chữ số biểu tượng bằng văn bản có liên quan đến FA CSO bên phải, ADC CSO ở cả hai bên, FA CC và MTR CSO bên phải, trong khi Bài kiểm tra các phương thức chữ số biểu tượng bằng miệng có liên quan đến FA, ADC và MTR CSO bên phải. Bài kiểm tra vẽ đường mòn, Phần B, có liên quan đến FA và MTR CSO bên phải. KẾT LUẬN Các phương thức chụp ảnh thần kinh tiên tiến như chụp ảnh tensor khuếch tán và chụp ảnh truyền từ chứng minh những thay đổi đáng kể về chất trắng, có liên quan đến tốc độ xử lý. Các kỹ thuật này có thể hữu ích trong việc định lượng mức độ chấn thương ngay cả ở chất trắng có vẻ bình thường sau TBI."} +{"idx": 1990122, "en": "The preparation of high-yield influenza H5N1 vaccine strains is challenging for researchers and manufacturers. Here, we used reverse genetics to generate a high-yield avian influenza vaccine strain based on a novel avian influenza virus. A high-yield attenuated recombinant H5N3 virus (rH5N3-DL) was prepared from the HA gene of A/Goose/Anhui/08 (H5N1), modified by deletion of the multiple basic amino acids at the cleavage site, the NA gene from A/Duck/Germany/1215/73 (H2N3), and the six internal genes from the high-yield A/Goose/Dalian/3/01 (H9N2) virus. rH5N3-DL grew to high HA titers (1:2048) in eggs, eight times those of the parental H5N1 virus, and four times higher than that of rH5N3-PR8 (six internal genes from the high-yield PR8). Infection tests demonstrated that rH5N3-DL was avirulent in chickens, chicken embryos, and mice. rH5N3-DL-vaccinated chickens were fully protected against the morbidity and mortality of a lethal challenge with homologous A/Goose/Anhui/08, but only 80% of chickens were protected after challenge with heterologous A/Goose/Guangdong/1/96. The N3 neuraminidase marker distinguishes rH5N3-DL-vaccinated from H5N1-infected animals. rH5N3-DL is thus a promising vaccine candidate to combat highly pathogenic avian influenza virus infections. The A/Goose/Dalian/3/01 virus could be a promising candidate as providing internal genes donors with high-yield properties in reverse-genetics system and might be applicable for future avian influenza vaccine development.", "gemini": "Việc chuẩn bị các chủng vắc-xin cúm H5N1 có năng suất cao là một thách thức đối với các nhà nghiên cứu và nhà sản xuất. Ở đây, chúng tôi đã sử dụng di truyền học ngược để tạo ra một chủng vắc-xin cúm gia cầm năng suất cao dựa trên một loại vi-rút cúm gia cầm mới. Một loại vi-rút H5N3 tái tổ hợp giảm độc lực năng suất cao (rH5N3-DL) đã được tạo ra từ gen HA của A/Goose/Anhui/08 (H5N1), được sửa đổi bằng cách xóa nhiều axit amin bazơ tại vị trí cắt, gen NA từ A/Duck/Germany/1215/73 (H2N3) và sáu gen bên trong từ vi-rút A/Goose/Dalian/3/01 (H9N2) năng suất cao. rH5N3-DL phát triển đến nồng độ HA cao (1:2048) trong trứng, gấp tám lần so với vi-rút H5N1 của cha mẹ và cao hơn bốn lần so với rH5N3-PR8 (sáu gen bên trong từ PR8 năng suất cao). Các xét nghiệm nhiễm trùng chứng minh rằng rH5N3-DL không độc đối với gà, phôi gà và chuột. Những con gà được tiêm vắc-xin rH5N3-DL được bảo vệ hoàn toàn khỏi bệnh tật và tử vong do thử thách gây tử vong với vi-rút A/Goose/Anhui/08 đồng loại, nhưng chỉ có 80% gà được bảo vệ sau khi thử thách với vi-rút A/Goose/Guangdong/1/96 khác loại. Đánh dấu neuraminidase N3 phân biệt động vật được tiêm vắc-xin rH5N3-DL với động vật bị nhiễm H5N1. Do đó, rH5N3-DL là một ứng cử viên vắc-xin đầy hứa hẹn để chống lại các bệnh nhiễm vi-rút cúm gia cầm có độc lực cao. Vi-rút A/Goose/Dalian/3/01 có thể là một ứng cử viên đầy hứa hẹn khi cung cấp các gen bên trong cho các đặc tính năng suất cao trong hệ thống di truyền ngược và có thể được áp dụng cho việc phát triển vắc-xin cúm gia cầm trong tương lai."} +{"idx": 1990123, "en": "OBJECTIVE To improve oral interpretation of metaphors by patients with traumatic brain injury (TBI). DESIGN Both single subject experimental design and group analysis. SETTING Patients' homes. PARTICIPANTS Eight adult patients with moderate to severe traumatic brain injury sustained 3 to 20 years before testing. INTERVENTION The Metaphor Training Program consisted typically of 10 baseline sessions, 3 to 9 1-hour sessions of structured intervention, and 10 posttraining baseline sessions. Training used extensive practice with simple graphic displays to illustrate semantic associations. MAIN OUTCOME MEASURES Quality of orally produced metaphor interpretation and accuracy of line orientation judgments served as dependent measures obtained during baseline, training, posttraining, and at a 3- to 4-month follow-up. Untrained line orientation judgments provided a control measure. RESULTS Group data showed significant improvement in metaphor interpretation but not in line orientation. Six of 8 patients individually demonstrated significant improvement in metaphor interpretation. Gains persisted for 3 of the 6 patients at the 3- to 4-month follow-up. CONCLUSION The Metaphor Training Program can improve cognitive-communication performance for individuals with moderate to severe traumatic brain injury. Results support the potential for treating patients' residual cognitive-linguistic deficits.", "gemini": "MỤC TIÊU Cải thiện khả năng diễn giải ẩn dụ bằng lời của những bệnh nhân bị chấn thương sọ não (TBI). THIẾT KẾ Thiết kế thử nghiệm đối tượng đơn lẻ và phân tích nhóm. BỐI CẢNH Nhà của bệnh nhân. NGƯỜI THAM GIA Tám bệnh nhân trưởng thành bị chấn thương sọ não từ trung bình đến nặng trong vòng 3 đến 20 năm trước khi thử nghiệm. CAN THIỆP Chương trình đào tạo ẩn dụ thường bao gồm 10 buổi cơ sở, 3 đến 9 buổi can thiệp có cấu trúc kéo dài 1 giờ và 10 buổi cơ sở sau đào tạo. Đào tạo sử dụng thực hành mở rộng với các màn hình đồ họa đơn giản để minh họa các liên tưởng ngữ nghĩa. CÁC BIỆN PHÁP KẾT QUẢ CHÍNH Chất lượng diễn giải ẩn dụ được tạo ra bằng lời và độ chính xác của các đánh giá định hướng đường thẳng đóng vai trò là các biện pháp phụ thuộc thu được trong quá trình đánh giá cơ sở, đào tạo, sau đào tạo và theo dõi sau 3 đến 4 tháng. Các đánh giá định hướng đường thẳng chưa được đào tạo cung cấp biện pháp kiểm soát. KẾT QUẢ Dữ liệu nhóm cho thấy sự cải thiện đáng kể trong việc diễn giải ẩn dụ nhưng không phải trong định hướng đường thẳng. Sáu trong số 8 bệnh nhân được cải thiện đáng kể về khả năng diễn giải ẩn dụ. Mức tăng vẫn duy trì ở 3 trong số 6 bệnh nhân khi theo dõi sau 3 đến 4 tháng. KẾT LUẬN Chương trình đào tạo ẩn dụ có thể cải thiện hiệu suất giao tiếp nhận thức cho những người bị chấn thương sọ não từ trung bình đến nặng. Kết quả hỗ trợ tiềm năng điều trị các khiếm khuyết về nhận thức-ngôn ngữ còn sót lại của bệnh nhân."} +{"idx": 1990124, "en": "Few epidemiological studies have examined the potential protective effects of physical activity on insomnia. The authors thus evaluated the association between physical activity and insomnia in a large population-based study in Shizuoka, Japan. Individual data were obtained from participants in an ongoing cohort study. A total of 14,001 older residents who completed questionnaires were followed for 3 yr. Of these, 10,211 and 3,697 participants were eligible for the cross-sectional and longitudinal analyses, respectively. The authors obtained information about the frequency of physical activity and insomnia. Then, the adjusted odds ratios and 95% confidence intervals between physical activity and insomnia were estimated. Habitual physical activity was related to lower prevalence of insomnia. Frequent physical activity also reduced the incidence of insomnia, especially difficulty maintaining sleep. For elderly people with sufficient mobility and no preexisting disease, high-frequency physical activity (e.g., 5 or more days/wk) may help reduce insomnia.", "gemini": "Ít nghiên cứu dịch tễ học nào xem xét các tác động bảo vệ tiềm tàng của hoạt động thể chất đối với chứng mất ngủ. Do đó, các tác giả đã đánh giá mối liên quan giữa hoạt động thể chất và chứng mất ngủ trong một nghiên cứu lớn dựa trên dân số tại Shizuoka, Nhật Bản. Dữ liệu cá nhân được lấy từ những người tham gia vào một nghiên cứu nhóm đang diễn ra. Tổng cộng có 14.001 cư dân lớn tuổi đã hoàn thành bảng câu hỏi được theo dõi trong 3 năm. Trong số những người này, có 10.211 và 3.697 người tham gia đủ điều kiện để phân tích theo chiều ngang và theo chiều dọc. Các tác giả đã thu thập thông tin về tần suất hoạt động thể chất và chứng mất ngủ. Sau đó, tỷ lệ cược đã điều chỉnh và khoảng tin cậy 95% giữa hoạt động thể chất và chứng mất ngủ được ước tính. Hoạt động thể chất thường xuyên có liên quan đến tỷ lệ mắc chứng mất ngủ thấp hơn. Hoạt động thể chất thường xuyên cũng làm giảm tỷ lệ mắc chứng mất ngủ, đặc biệt là khó duy trì giấc ngủ. Đối với người cao tuổi có khả năng vận động tốt và không mắc bệnh trước đó, hoạt động thể chất có tần suất cao (ví dụ: 5 ngày/tuần trở lên) có thể giúp giảm chứng mất ngủ."} +{"idx": 1990125, "en": "OBJECTIVE To examine the differential effect of neurobehavioral impairments (cognitive, behavioral, and social) on family functioning, family roles, and psychological distress in male versus female caregivers of relatives with severe traumatic brain injury (TBI). DESIGN Structural equation modeling with multigroup analysis conducted in a cross-sectional sample to test an established theoretical model. PARTICIPANTS An aggregated sample of 122 caregivers (46 male, 76 female) of people with severe TBI. The sample comprised 64 spouses and 58 parents (29 parental couples) of 93 persons with TBI. MEASURES Neurobehavioral Problem Checklist; Family Assessment Device; and Brief Symptom Inventory. RESULTS Structural equation modeling showed that the proposed model had acceptable fit indices for the combined sample. Multigroup analysis indicated that both male and female caregivers (i) responded similarly to the neurobehavioral impairments experienced by the injured relative and (ii) reported behavior having a direct effect on family functioning, which, in turn, increased psychological distress. However, the effect of disrupted family functioning was more influential on the level of distress in male caregivers than in female caregivers. CONCLUSION Evidence was found for gender-specific pathways underlying the psychological distress of male versus female caregivers. Such findings can assist in tailoring family support strategies so that they cater for caregivers of both genders.", "gemini": "MỤC TIÊU Kiểm tra sự khác biệt về tác động của các khiếm khuyết về hành vi thần kinh (nhận thức, hành vi và xã hội) đối với chức năng gia đình, vai trò gia đình và đau khổ về mặt tâm lý ở những người chăm sóc nam so với nữ của những người thân bị chấn thương sọ não (TBI) nghiêm trọng. THIẾT KẾ Mô hình phương trình cấu trúc với phân tích đa nhóm được tiến hành trong một mẫu cắt ngang để kiểm tra một mô hình lý thuyết đã được thiết lập. NGƯỜI THAM GIA Một mẫu tổng hợp gồm 122 người chăm sóc (46 nam, 76 nữ) của những người bị TBI nghiêm trọng. Mẫu bao gồm 64 người phối ngẫu và 58 cha mẹ (29 cặp cha mẹ) của 93 người bị TBI. CÁC BIỆN PHÁP Danh sách kiểm tra vấn đề hành vi thần kinh; Thiết bị đánh giá gia đình; và Bản kiểm kê triệu chứng ngắn gọn. KẾT QUẢ Mô hình phương trình cấu trúc cho thấy mô hình được đề xuất có các chỉ số phù hợp chấp nhận được cho mẫu kết hợp. Phân tích đa nhóm chỉ ra rằng cả những người chăm sóc nam và nữ (i) phản ứng tương tự đối với các khiếm khuyết về hành vi thần kinh mà người thân bị thương gặp phải và (ii) báo cáo hành vi có tác động trực tiếp đến chức năng gia đình, từ đó làm tăng đau khổ về mặt tâm lý. Tuy nhiên, tác động của chức năng gia đình bị gián đoạn có ảnh hưởng lớn hơn đến mức độ đau khổ ở những người chăm sóc nam so với những người chăm sóc nữ. KẾT LUẬN Đã tìm thấy bằng chứng về các con đường riêng biệt theo giới tính làm nền tảng cho sự đau khổ về mặt tâm lý của những người chăm sóc nam so với nữ. Những phát hiện như vậy có thể hỗ trợ trong việc điều chỉnh các chiến lược hỗ trợ gia đình để đáp ứng cho những người chăm sóc của cả hai giới."} +{"idx": 1990126, "en": "BACKGROUND increased provision of orthogeriatric expertise for patients with femoral fractures has led to implementation of 'Do Not Attempt Resuscitation' (DNAR) decisions prior to anaesthesia and surgery for fixation of their fractures. Review and modification of the DNAR decision by the medical team is necessary before surgery and is recommended by guidelines from the Association of Anaesthetists of Great Britain and Ireland (AAGBI) and the General Medical Council. METHODS over a 17-month period, DNAR decisions were already present or were implemented for the first time in 22 patients prior to scheduled surgical fixation of their femoral fractures. Data were collected prospectively on each patient's management, including modification of their DNAR decision, and outcome at 30 days and 1 year. RESULTS two patients died prior to surgery. In eight of the 20 patients who underwent surgical fixation, there was no documentation regarding the status of the DNAR decision in the perioperative period. The 30-day mortality rate for those undergoing surgery was 15% (3/20). At 1 year, eight patients survived with six living in their own homes. CONCLUSIONS despite the favourable outcomes for hip fracture patients with pre-existing DNAR decisions, this audit showed inadequate review and documentation of the DNAR decision in advance of surgery.", "gemini": "BỐI CẢNH việc cung cấp chuyên môn chỉnh hình ngày càng tăng cho những bệnh nhân bị gãy xương đùi đã dẫn đến việc thực hiện các quyết định \"Không cố gắng hồi sức\" (DNAR) trước khi gây mê và phẫu thuật để cố định các vết gãy của họ. Đội ngũ y tế cần phải xem xét và sửa đổi quyết định DNAR trước khi phẫu thuật và được khuyến nghị bởi các hướng dẫn từ Hiệp hội bác sĩ gây mê của Vương quốc Anh và Ireland (AAGBI) và Hội đồng y khoa chung. PHƯƠNG PHÁP trong khoảng thời gian 17 tháng, các quyết định DNAR đã có hoặc được thực hiện lần đầu tiên ở 22 bệnh nhân trước khi phẫu thuật cố định gãy xương đùi theo lịch trình. Dữ liệu được thu thập có triển vọng về việc quản lý của từng bệnh nhân, bao gồm cả việc sửa đổi quyết định DNAR của họ và kết quả sau 30 ngày và 1 năm. KẾT QUẢ hai bệnh nhân đã tử vong trước khi phẫu thuật. Trong số 20 bệnh nhân đã phẫu thuật cố định, có tám bệnh nhân không có hồ sơ về tình trạng quyết định DNAR trong giai đoạn quanh phẫu thuật. Tỷ lệ tử vong trong vòng 30 ngày đối với những bệnh nhân được phẫu thuật là 15% (3/20). Sau 1 năm, tám bệnh nhân vẫn sống và sáu bệnh nhân sống tại nhà riêng. KẾT LUẬN bất chấp kết quả thuận lợi đối với những bệnh nhân gãy xương hông có quyết định DNAR trước đó, cuộc kiểm toán này cho thấy việc xem xét và ghi chép không đầy đủ về quyết định DNAR trước khi phẫu thuật."} +{"idx": 1990127, "en": "BACKGROUND STOPP/START was formulated to identify potentially inappropriate prescribing (PIP) and potential prescribing omissions (PPOs) in older people. The purpose of this study was to determine the prevalence of PIP and PPO in older Irish patients in residential care using STOPP/START. METHODS data were collected prospectively from seven publicly funded nursing homes within the Munster Region of Ireland over 3 weeks. Data recorded included: current medication, current medical conditions, previous medical conditions, biochemistry, sex and age. STOPP/START was applied to each patient record. RESULTS of the 313 patients recruited, 74.4% (233) were female, mean age (± SD) 84.4 (± 7.5) years. The total number of medicines prescribed was 2,555 [range: 1-16; median: 8 (IQR 6-10 )]. STOPP identified 329 instances of PIP in 187 (59.8%) patients and START identified 199 PPOs in 132 (42.2%) patients. The number of medicines prescribed was positively associated with PIP identified by STOPP (rs = 0.303, P < 0.01). Age, sex and the number of medicines prescribed were not associated with prescribing omissions using START. CONCLUSIONS a high proportion of patients recruited were prescribed at least one potentially inappropriate medicine, or had an omission of a clinically indicated medicine. Incorporating these tools into every-day practice could play a pivotal role in improving prescribing in this cohort.", "gemini": "BỐI CẢNH STOPP/START được xây dựng để xác định kê đơn không phù hợp có thể xảy ra (PIP) và các trường hợp bỏ sót kê đơn có thể xảy ra (PPO) ở người cao tuổi. Mục đích của nghiên cứu này là xác định tỷ lệ mắc PIP và PPO ở bệnh nhân lớn tuổi người Ireland đang được chăm sóc tại nhà bằng cách sử dụng STOPP/START. PHƯƠNG PHÁP dữ liệu được thu thập có triển vọng từ bảy viện dưỡng lão do nhà nước tài trợ trong khu vực Munster của Ireland trong hơn 3 tuần. Dữ liệu được ghi lại bao gồm: thuốc hiện tại, tình trạng bệnh hiện tại, tình trạng bệnh trước đó, sinh hóa, giới tính và tuổi tác. STOPP/START được áp dụng cho hồ sơ bệnh án của từng bệnh nhân. KẾT QUẢ trong số 313 bệnh nhân được tuyển dụng, 74,4% (233) là nữ, tuổi trung bình (± SD) là 84,4 (± 7,5) tuổi. Tổng số loại thuốc được kê đơn là 2.555 [phạm vi: 1-16; trung vị: 8 (IQR 6-10)]. STOPP xác định 329 trường hợp PIP ở 187 (59,8%) bệnh nhân và START xác định 199 trường hợp PPO ở 132 (42,2%) bệnh nhân. Số loại thuốc được kê đơn có liên quan tích cực với PIP được STOPP xác định (rs = 0,303, P < 0,01). Tuổi tác, giới tính và số loại thuốc được kê đơn không liên quan đến việc bỏ sót kê đơn khi sử dụng START. KẾT LUẬN một tỷ lệ lớn bệnh nhân được tuyển dụng được kê đơn ít nhất một loại thuốc có khả năng không phù hợp hoặc bị bỏ sót một loại thuốc được chỉ định lâm sàng. Việc kết hợp các công cụ này vào thực hành hàng ngày có thể đóng vai trò quan trọng trong việc cải thiện việc kê đơn trong nhóm bệnh nhân này."} +{"idx": 1990128, "en": "Coronary artery disease, one of the leading causes of worldwide adult mortality, is most commonly atherosclerotic in pathogenesis. Nonatherosclerotic etiologies are quite rare. In the latter category, infective arteritis or infective vasculitis of the coronary arteries is a very rare but well-recognized subtype, usually discovered at autopsy. In this article, we present the clinicopathologic necropsy data of 10 patients in whom infective coronary arteritis was the leading cause of death. Among the 10 cases, the male/female ratio was 6:4, and with the exception of a 2-year-old female child, all the other patients were adults with an age range of 26 to 59 years. Of the 10 cases, 6 had infective endocarditis along with history of rheumatic heart disease in 3 patients, whereas 2 other patients had strong clinical suspicion of bacteremia or septicemia. The remaining 2 cases had preexisting coronary atherosclerosis with a history of stent placement in 1 of them. All our cases showed on histopathology acute obliterative inflammatory infiltrate consisting mainly of neutrophils along with bacterial colonies (in most of them) involving the epicardial and intramural coronary arteries. To the best of our knowledge, this is the largest series of infective coronary arteritis to be reported in the world.", "gemini": "Bệnh động mạch vành, một trong những nguyên nhân hàng đầu gây tử vong ở người lớn trên toàn thế giới, thường gặp nhất là do bệnh xơ vữa động mạch. Nguyên nhân không do xơ vữa động mạch khá hiếm gặp. Trong thể loại sau, viêm động mạch nhiễm trùng hoặc viêm mạch nhiễm trùng ở động mạch vành là một phân nhóm rất hiếm nhưng được công nhận, thường được phát hiện khi khám nghiệm tử thi. Trong bài viết này, chúng tôi trình bày dữ liệu khám nghiệm tử thi lâm sàng bệnh học của 10 bệnh nhân mà viêm động mạch vành nhiễm trùng là nguyên nhân tử vong hàng đầu. Trong số 10 trường hợp, tỷ lệ nam/nữ là 6:4, và ngoại trừ một trẻ em gái 2 tuổi, tất cả các bệnh nhân khác đều là người lớn trong độ tuổi từ 26 đến 59. Trong số 10 trường hợp, 6 trường hợp bị viêm nội tâm mạc nhiễm trùng cùng với tiền sử bệnh tim thấp ở 3 bệnh nhân, trong khi 2 bệnh nhân khác có nghi ngờ lâm sàng mạnh về tình trạng nhiễm khuẩn huyết hoặc nhiễm trùng huyết. 2 trường hợp còn lại có bệnh xơ vữa động mạch vành từ trước với tiền sử đặt stent ở 1 trường hợp. Tất cả các trường hợp của chúng tôi đều cho thấy trên mô bệnh học thâm nhiễm viêm tắc cấp tính chủ yếu bao gồm các tế bào bạch cầu trung tính cùng với các khuẩn lạc vi khuẩn (ở hầu hết các trường hợp) liên quan đến các động mạch vành ngoài tim và trong thành tim. Theo hiểu biết của chúng tôi, đây là loạt trường hợp viêm động mạch vành nhiễm trùng lớn nhất được báo cáo trên thế giới."} +{"idx": 1990129, "en": "OBJECTIVE We conducted a case-control study to examine if short-term glucose control is related to healthcare-associated bloodstream infections (BSI), urinary tract infections (UTI), and pneumonia in hospitalized adults with diabetes. SETTING AND PATIENTS We analyzed 205 BSI, 510 UTI, and 109 pneumonia cases and 989, 2463, and 543 controls matched by age, sex and hospital stay seen at a large healthcare system in Manhattan from 2006 to 2008. METHODS We examined whether infection risk was associated with serum glucose measured at admission and within 2 days to infection, using conditional logistic regression. Co-morbidities, immunosuppressive medications, prior hospitalizations, and insertion of indwelling devices were considered as potential confounders. RESULTS Admission glucose level was not associated with infection. Glucose levels of ≥ 110 mg/dL measured within 2 days to infection were associated with BSI (Odds ratios from 2.04 to 2.67). Glucose level of ≥ 180 mg/dL was associated with pneumonia (Odds ratio=2.30). Decrease in glucose levels from admission to the infection was greater for controls than for infected cases. CONCLUSION Healthcare-associated BSI and pneumonia were associated with glucose levels prior to infection diagnosis, but not with glucose levels at admission. Persistently high glucose level could be an indication of an underlying undiagnosed infection.", "gemini": "MỤC TIÊU Chúng tôi đã tiến hành một nghiên cứu ca chứng để xem xét liệu kiểm soát glucose trong thời gian ngắn có liên quan đến nhiễm trùng huyết liên quan đến chăm sóc sức khỏe (BSI), nhiễm trùng đường tiết niệu (UTI) và viêm phổi ở những người lớn nhập viện mắc bệnh tiểu đường hay không. HOÀN CẢNH VÀ BỆNH NHÂN Chúng tôi đã phân tích 205 trường hợp BSI, 510 trường hợp UTI và 109 trường hợp viêm phổi và 989, 2463 và 543 ca đối chứng được ghép theo độ tuổi, giới tính và thời gian nằm viện được nhìn thấy tại một hệ thống chăm sóc sức khỏe lớn ở Manhattan từ năm 2006 đến 2008. PHƯƠNG PHÁP Chúng tôi đã kiểm tra xem nguy cơ nhiễm trùng có liên quan đến lượng glucose trong huyết thanh được đo khi nhập viện và trong vòng 2 ngày sau khi nhiễm trùng hay không, bằng cách sử dụng hồi quy logistic có điều kiện. Các bệnh đi kèm, thuốc ức chế miễn dịch, nhập viện trước đó và việc lắp các thiết bị nội trú được coi là các yếu tố gây nhiễu tiềm ẩn. KẾT QUẢ Nồng độ glucose khi nhập viện không liên quan đến nhiễm trùng. Nồng độ glucose ≥ 110 mg / dL được đo trong vòng 2 ngày sau khi nhiễm trùng có liên quan đến BSI (Tỷ lệ cược từ 2,04 đến 2,67). Nồng độ glucose ≥ 180 mg / dL có liên quan đến viêm phổi (Tỷ lệ cược = 2,30). Giảm nồng độ glucose từ khi nhập viện đến khi nhiễm trùng lớn hơn đối với các ca đối chứng so với các ca bị nhiễm trùng. KẾT LUẬN BSI và viêm phổi liên quan đến chăm sóc sức khỏe có liên quan đến nồng độ glucose trước khi chẩn đoán nhiễm trùng, nhưng không liên quan đến nồng độ glucose khi nhập viện. Nồng độ glucose cao liên tục có thể là dấu hiệu của một nhiễm trùng tiềm ẩn chưa được chẩn đoán."} +{"idx": 1990130, "en": "BACKGROUND Matrine is one of the major alkaloids extracted from Sophora flavescens and has been used clinically for breast cancer with notable therapeutic efficacy in China. However, the mechanisms are still largely unknown. METHODS Cell viability was analyzed by MTT assay. After MCF-7 cells were treated with matrine for 48h, apoptosis was detected by flow cytometry, TUNEL assay and transmission electron microscopy, and the cell cycle distribution was also analyzed by flow cytometry. Further, the expression of PTEN, pAkt, Akt, pBad, Bad, p21(/WAF1/CIP1), and p27(/KIP1) was determined by Western blot. Changes of miR-21 level were quantified by real-time RT-PCR. After miR-21 was transfected in MCF-7 cells, PTEN protein level was measured by Western blot. RESULTS Matrine inhibited MCF-7 cell growth in a concentration-and time-dependent manner, by inducing apoptosis and cell cycle arrest at G(1)/S phase. Matrine up-regulated PTEN by downregulating miR-21 which in turn dephosphorylated Akt, resulting in accumulation of Bad, p21(/WAF1/CIP1) and p27(/KIP1). CONCLUSION Our study unraveled, for the first time, the ability of matrine to suppress breast cancer growth and elucidated the miR-21/PTEN/Akt pathway as a signaling mechanism for the anti-cancer action of matrine. Our findings also reinforce the notion that miRNAs can act as mediators of the therapeutic efficacy of natural medicines.", "gemini": "BỐI CẢNH Matrine là một trong những alkaloid chính được chiết xuất từ Sophora flavescens và đã được sử dụng lâm sàng cho bệnh ung thư vú với hiệu quả điều trị đáng chú ý ở Trung Quốc. Tuy nhiên, các cơ chế vẫn chưa được biết đến nhiều. PHƯƠNG PHÁP Khả năng sống của tế bào được phân tích bằng xét nghiệm MTT. Sau khi các tế bào MCF-7 được xử lý bằng matrine trong 48 giờ, quá trình apoptosis được phát hiện bằng phương pháp đo lưu lượng tế bào, xét nghiệm TUNEL và kính hiển vi điện tử truyền qua, và sự phân bố chu kỳ tế bào cũng được phân tích bằng phương pháp đo lưu lượng tế bào. Hơn nữa, biểu hiện của PTEN, pAkt, Akt, pBad, Bad, p21 (/ WAF1 / CIP1) và p27 (/ KIP1) được xác định bằng phương pháp Western blot. Những thay đổi của mức độ miR-21 được định lượng bằng phương pháp RT-PCR thời gian thực. Sau khi miR-21 được chuyển vào tế bào MCF-7, mức độ protein PTEN được đo bằng phương pháp Western blot. KẾT QUẢ Matrine ức chế sự phát triển của tế bào MCF-7 theo cách phụ thuộc vào nồng độ và thời gian, bằng cách gây ra quá trình apoptosis và bắt giữ chu kỳ tế bào ở pha G (1) / S. Matrine điều hòa PTEN bằng cách hạ điều hòa miR-21, từ đó khử phosphoryl hóa Akt, dẫn đến tích tụ Bad, p21 (/ WAF1 / CIP1) và p27 (/ KIP1). KẾT LUẬN Nghiên cứu của chúng tôi lần đầu tiên làm sáng tỏ khả năng của matrine trong việc ức chế sự phát triển của ung thư vú và làm sáng tỏ con đường miR-21 / PTEN / Akt như một cơ chế truyền tín hiệu cho tác dụng chống ung thư của matrine. Những phát hiện của chúng tôi cũng củng cố quan điểm cho rằng miRNA có thể hoạt động như chất trung gian trong hiệu quả điều trị của các loại thuốc tự nhiên."} +{"idx": 1990131, "en": "A genome-wide association study, conducted among women of European ancestry, has identified two single-nucleotide polymorphisms (SNPs) rs4415084 (T>C) and rs10941679 (A>G) at chromosome 5p12 were associated with risk of breast cancer, suggesting that genetic variants in this region may have a role in the development of breast cancer. To investigate the associations between SNPs at 5p12 and risk of breast cancer in the Chinese population, we conducted a fine-mapping in 5p12 using a haplotype-tagging SNP approach and genotyped these SNPs with a case-control study consisting of 878 cases and 900 controls. We found that the two risk SNPs reported in the European population were neither associated with breast cancer risk in our Chinese population, nor did the fine-mapping SNPs after controlling multiple comparison.", "gemini": "Một nghiên cứu liên kết toàn bộ hệ gen, được thực hiện trên những phụ nữ có tổ tiên là người châu Âu, đã xác định được hai đa hình nucleotide đơn (SNP) rs4415084 (T> C) và rs10941679 (A> G) ở nhiễm sắc thể 5p12 có liên quan đến nguy cơ ung thư vú, cho thấy rằng các biến thể di truyền ở vùng này có thể có vai trò trong sự phát triển của ung thư vú. Để nghiên cứu mối liên quan giữa các SNP ở 5p12 và nguy cơ ung thư vú ở quần thể Trung Quốc, chúng tôi đã tiến hành lập bản đồ chi tiết ở 5p12 bằng cách sử dụng phương pháp SNP gắn thẻ kiểu gen và định kiểu gen các SNP này bằng một nghiên cứu ca chứng bao gồm 878 ca và 900 ca đối chứng. Chúng tôi phát hiện ra rằng hai SNP nguy cơ được báo cáo ở quần thể châu Âu không liên quan đến nguy cơ ung thư vú ở quần thể Trung Quốc của chúng tôi, cũng như các SNP lập bản đồ chi tiết sau khi kiểm soát nhiều phép so sánh."} +{"idx": 1990132, "en": "In the nonrecombining region of the Y-chromosome, there are single-nucleotide polymorphisms (Y-SNPs) that establish haplogroups with particular geographical origins (European, African, Native American, etc.). The complex process of admixture that gave rise to the majority of the current Mexican population (~93%), known as Mestizos, can be examined with Y-SNPs to establish their paternal ancestry and population structure. We analyzed 18 Y-SNPs in 659 individuals from 10 Mexican-Mestizo populations from different regions of the country. In the total population sample, paternal ancestry was predominately European (64.9%), followed by Native American (30.8%) and African (4.2%). However, the European ancestry was prevalent in the north and west (66.7-95%) and, conversely, Native American ancestry increased in the center and southeast (37-50%), whereas the African ancestry was low and relatively homogeneous (0-8.8%). Although this paternal landscape concurs with previous studies based on genome-wide SNPs and autosomal short tandem repeats (STRs), this pattern contrasts with the maternal ancestry, mainly of Native American origin, based on maternal lineages haplogroups. In agreement with historical records, these results confirm a strong gender-biased admixture history between European males and Native American females that gave rise to Mexican-Mestizos. Finally, pairwise comparisons and analysis of molecular variance tests demonstrated significant population structure (F(ST)=4.68%; P<0.00005), delimiting clusters that were geographically defined as the following: north-west, center-south and southeast.", "gemini": "Trong vùng không tái tổ hợp của nhiễm sắc thể Y, có các đa hình một nucleotide (Y-SNP) thiết lập các nhóm đơn bội có nguồn gốc địa lý cụ thể (châu Âu, châu Phi, thổ dân châu Mỹ, v.v.). Quá trình pha trộn phức tạp tạo nên phần lớn dân số Mexico hiện tại (~ 93%), được gọi là người Mestizos, có thể được kiểm tra bằng Y-SNP để thiết lập nguồn gốc tổ tiên và cấu trúc dân số của họ. Chúng tôi đã phân tích 18 Y-SNP ở 659 cá thể từ 10 quần thể người Mestizo Mexico từ các vùng khác nhau của đất nước. Trong tổng số mẫu dân số, nguồn gốc tổ tiên là người châu Âu chiếm ưu thế (64,9%), tiếp theo là người Mỹ bản địa (30,8%) và người châu Phi (4,2%). Tuy nhiên, nguồn gốc tổ tiên là người châu Âu chiếm ưu thế ở phía bắc và phía tây (66,7-95%) và ngược lại, nguồn gốc tổ tiên là người Mỹ bản địa tăng lên ở trung tâm và đông nam (37-50%), trong khi nguồn gốc tổ tiên là người châu Phi thấp và tương đối đồng nhất (0-8,8%). Mặc dù bức tranh về nguồn gốc tổ tiên này phù hợp với các nghiên cứu trước đây dựa trên các SNP trên toàn bộ hệ gen và các lặp lại tuần tự ngắn trên nhiễm sắc thể thường (STR), nhưng mô hình này trái ngược với nguồn gốc tổ tiên là người mẹ, chủ yếu có nguồn gốc từ người Mỹ bản địa, dựa trên các nhóm đơn bội theo dòng mẹ. Phù hợp với các ghi chép lịch sử, những kết quả này xác nhận một lịch sử pha trộn thiên vị giới tính mạnh mẽ giữa nam giới châu Âu và phụ nữ bản địa châu Mỹ, dẫn đến sự ra đời của người Mestizos Mexico. Cuối cùng, các so sánh từng cặp và phân tích các xét nghiệm độ biến động phân tử đã chứng minh cấu trúc dân số đáng kể (F(ST) = 4,68%; P < 0,00005), phân định các cụm được xác định về mặt địa lý như sau: tây bắc, trung nam và đông nam."} +{"idx": 1990133, "en": "Neutral lipid storage disease with myopathy (NLSDM) referred to those neutral lipid storage disease (NLSD) patients with myopathy but without ichthyosis. Recently, NLSDM has been attributed to mutations in the PNPLA2 gene. Until now, 19 patients with PNPLA2 mutations have been reported. In the present study, we describe the clinical and genetic findings in three Chinese patients with NLSDM. Sequence analysis of PNPLA2 gene was performed. In our patients we identified four novel mutations in the PNPLA2 gene including two splicing mutations. The identification and study of mutations found in PNPLA2 is also particularly important to define the clinical spectrum and genotype-phenotype correlations of the PNPLA2 gene.", "gemini": "Bệnh tích trữ lipid trung tính với bệnh cơ (NLSDM) dùng để chỉ những bệnh nhân mắc bệnh tích trữ lipid trung tính (NLSD) có bệnh cơ nhưng không bị bệnh vảy cá. Gần đây, NLSDM đã được quy cho các đột biến trong gen PNPLA2. Cho đến nay, đã có 19 bệnh nhân có đột biến PNPLA2 được báo cáo. Trong nghiên cứu hiện tại, chúng tôi mô tả các phát hiện lâm sàng và di truyền ở ba bệnh nhân người Trung Quốc mắc NLSDM. Phân tích trình tự gen PNPLA2 đã được thực hiện. Ở những bệnh nhân của chúng tôi, chúng tôi đã xác định được bốn đột biến mới trong gen PNPLA2 bao gồm hai đột biến ghép nối. Việc xác định và nghiên cứu các đột biến được tìm thấy trong PNPLA2 cũng đặc biệt quan trọng để xác định phổ lâm sàng và mối tương quan kiểu gen-kiểu hình của gen PNPLA2."} +{"idx": 1990134, "en": "Traditional approaches for gene mapping from candidate gene studies to positional cloning strategies have been applied for Mendelian disorders. Since 2005, next-generation sequencing (NGS) technologies are improving as rapid, high-throughput and cost-effective approaches to fulfill medical sciences and research demands. Using NGS, the underlying causative genes are directly distinguished via a systematic filtering, in which the identified gene variants are checked for novelty and functionality. During the past 2 years, the role of more than 100 genes has been distinguished in rare Mendelian disorders by means of whole-exome sequencing (WES). Combination of WES with traditional approaches, consistent with linkage analysis, has had the greatest impact on those disorders following autosomal mode of inheritance; in more than 60 identified genes, the causal variants have been transmitted at homozygous or compound heterozygous state. Recent literatures focusing on identified new causal genes in Mendelian disorders using WES are reviewed in the present survey.", "gemini": "Các phương pháp tiếp cận truyền thống để lập bản đồ gen từ các nghiên cứu gen ứng viên đến các chiến lược nhân bản định vị đã được áp dụng cho các rối loạn theo kiểu Mendel. Kể từ năm 2005, các công nghệ giải trình tự thế hệ tiếp theo (NGS) đang được cải thiện như các phương pháp nhanh chóng, thông lượng cao và tiết kiệm chi phí để đáp ứng nhu cầu nghiên cứu và khoa học y tế. Sử dụng NGS, các gen gây bệnh cơ bản được phân biệt trực tiếp thông qua quá trình lọc có hệ thống, trong đó các biến thể gen được xác định được kiểm tra về tính mới lạ và chức năng. Trong 2 năm qua, vai trò của hơn 100 gen đã được phân biệt trong các rối loạn theo kiểu Mendel hiếm gặp bằng phương pháp giải trình tự toàn bộ exome (WES). Sự kết hợp của WES với các phương pháp tiếp cận truyền thống, phù hợp với phân tích liên kết, đã có tác động lớn nhất đối với những rối loạn theo kiểu di truyền trội trên nhiễm sắc thể thường; trong hơn 60 gen được xác định, các biến thể gây bệnh đã được truyền ở trạng thái đồng hợp tử hoặc dị hợp tử kép. Các tài liệu gần đây tập trung vào các gen gây bệnh mới được xác định trong các rối loạn theo kiểu Mendel sử dụng WES được xem xét trong khảo sát hiện tại."} +{"idx": 1990135, "en": "Using functional magnetic resonance imaging, we show for the first time that levels of musical expertise stepwise modulate higher order brain functioning. This suggests that degree of training intensity drives such cerebral plasticity. Participants (non-musicians, amateurs, and expert musicians) listened to a comprehensive set of specifically composed string quartets with hierarchically manipulated endings. In particular, we implemented 2 irregularities at musical closure that differed in salience but were both within the tonality of the piece (in-key). Behavioral sensitivity scores (d') of both transgressions perfectly separated participants according to their level of musical expertise. By contrasting brain responses to harmonic transgressions against regular endings, functional brain imaging data showed compelling evidence for stepwise modulation of brain responses by both violation strength and expertise level in a fronto-temporal network hosting universal functions of working memory and attention. Additional independent testing evidenced an advantage in visual working memory for the professionals, which could be predicted by musical training intensity. The here introduced findings of brain plasticity demonstrate the progressive impact of musical training on cognitive brain functions that may manifest well beyond the field of music processing.", "gemini": "Sử dụng hình ảnh cộng hưởng từ chức năng, chúng tôi lần đầu tiên chứng minh rằng các cấp độ chuyên môn âm nhạc điều chỉnh từng bước chức năng não cấp cao hơn. Điều này cho thấy rằng mức độ cường độ đào tạo thúc đẩy tính dẻo của não. Những người tham gia (người không phải nhạc sĩ, người nghiệp dư và nhạc sĩ chuyên nghiệp) đã nghe một bộ tứ tấu đàn dây được sáng tác riêng với phần kết được thao tác theo thứ bậc. Đặc biệt, chúng tôi đã triển khai 2 điểm bất thường khi kết thúc âm nhạc có sự khác biệt về độ nổi bật nhưng đều nằm trong cung bậc của tác phẩm (in-key). Điểm số nhạy cảm về hành vi (d') của cả hai vi phạm đều tách biệt hoàn toàn những người tham gia theo trình độ chuyên môn âm nhạc của họ. Bằng cách đối chiếu các phản ứng của não đối với các vi phạm về hòa âm với các kết thúc thông thường, dữ liệu hình ảnh chức năng của não cho thấy bằng chứng thuyết phục về sự điều chỉnh từng bước các phản ứng của não theo cả mức độ vi phạm và mức độ chuyên môn trong mạng lưới trán thái lưu trữ các chức năng phổ quát của trí nhớ làm việc và sự chú ý. Các thử nghiệm độc lập bổ sung đã chứng minh lợi thế về trí nhớ làm việc trực quan đối với các chuyên gia, có thể được dự đoán bằng cường độ đào tạo âm nhạc. Những phát hiện được giới thiệu ở đây về tính dẻo của não chứng minh tác động tiến triển của đào tạo âm nhạc đối với các chức năng nhận thức của não có thể biểu hiện rõ ràng vượt ra ngoài lĩnh vực xử lý âm nhạc."} +{"idx": 1990136, "en": "The neuropeptide vasopressin is a key molecular mediator of social behavior in animals and humans, implicated in anxiety and autism. Social recognition, the ability to assess the familiarity of others, is essential for appropriate social interactions and enhanced by vasopressin; however, the neural mechanisms mediating this effect in humans are unknown. Using functional magnetic resonance imaging (fMRI) and an implicit social recognition matching task, we employed a double-blinded procedure in which 20 healthy male volunteers self-administered 40 UI of vasopressin or placebo intranasally, 45 min before performing the matching task in the scanner. In a random-effects fMRI analysis, we show that vasopressin induces a regionally specific alteration in a key node of the theory of mind network, the left temporoparietal junction, identifying a neurobiological mechanism for prosocial neuropeptide effects in humans that suggests novel treatment strategies.", "gemini": "Neuropeptide vasopressin là một chất trung gian phân tử chính của hành vi xã hội ở động vật và con người, có liên quan đến lo âu và tự kỷ. Nhận dạng xã hội, khả năng đánh giá mức độ quen thuộc của người khác, là điều cần thiết cho các tương tác xã hội phù hợp và được vasopressin tăng cường; tuy nhiên, cơ chế thần kinh trung gian tác dụng này ở người vẫn chưa được biết rõ. Sử dụng hình ảnh cộng hưởng từ chức năng (fMRI) và nhiệm vụ khớp nhận dạng xã hội ngầm, chúng tôi đã sử dụng một quy trình mù đôi trong đó 20 nam tình nguyện viên khỏe mạnh tự dùng 40 UI vasopressin hoặc giả dược qua đường mũi, 45 phút trước khi thực hiện nhiệm vụ khớp trong máy quét. Trong phân tích fMRI hiệu ứng ngẫu nhiên, chúng tôi chỉ ra rằng vasopressin gây ra sự thay đổi đặc hiệu theo khu vực ở một nút chính của mạng lý thuyết về tâm trí, điểm nối thái dương đỉnh trái, xác định một cơ chế thần kinh sinh học cho các tác dụng neuropeptide ủng hộ xã hội ở người, gợi ý các chiến lược điều trị mới."} +{"idx": 1990137, "en": "BACKGROUND Pleural empyema can be subdivided into 3 stages: exudative, multiloculated, and organizing. In the absence of clear septation, antibiotics plus simple drainage of pleural fluid is often sufficient treatment, whereas clear septation often requires more invasive treatment. OBJECTIVES The aim of this study was to report our experience and analyze the safety and efficacy of medical thoracoscopy in patients with multiloculated and organizing empyema. METHODS We performed a retrospective study reviewing the files of patients referred for empyema and treated by medical thoracoscopy at our department from July 2005 to February 2011. RESULTS A total of 41 patients with empyema were treated by medical thoracoscopy; empyema was free flowing in 9 patients (22%), multiloculated in 24 patients (58.5%), and organized in 8 patients (19.5%). Medical thoracoscopy was considered successful without further intervention in 35 of 41 patients (85.4%): all of the 9 patients with free-flowing fluid, 22 of the 24 patients with multiloculated empyema (91.7%), and only 4 of the 8 patients with organizing effusion (50%). CONCLUSIONS Our study confirms that multiloculated pleural empyema could safely and successfully be treated with medical thoracoscopy while organizing empyema can be resistant to drainage with medical thoracoscopy, requiring video-assisted thoracic surgery or open surgical decortications; among this population, the presence of separate 'pockets' not in apparent communication with each other often leads to a surgical approach.", "gemini": "BỐI CẢNH Tràn dịch màng phổi có thể được chia thành 3 giai đoạn: xuất dịch, đa nang và tổ chức. Trong trường hợp không có vách ngăn rõ ràng, kháng sinh cộng với dẫn lưu dịch màng phổi đơn giản thường là đủ để điều trị, trong khi vách ngăn rõ ràng thường đòi hỏi điều trị xâm lấn hơn. MỤC TIÊU Mục đích của nghiên cứu này là báo cáo kinh nghiệm của chúng tôi và phân tích tính an toàn và hiệu quả của nội soi màng phổi bằng phương pháp nội khoa ở những bệnh nhân bị tràn dịch màng phổi đa nang và tổ chức. PHƯƠNG PHÁP Chúng tôi đã thực hiện một nghiên cứu hồi cứu xem xét hồ sơ bệnh án của những bệnh nhân được chuyển đến vì tràn dịch màng phổi và được điều trị bằng nội soi màng phổi bằng phương pháp nội khoa tại khoa của chúng tôi từ tháng 7 năm 2005 đến tháng 2 năm 2011. KẾT QUẢ Tổng cộng 41 bệnh nhân bị tràn dịch màng phổi được điều trị bằng nội soi màng phổi bằng phương pháp nội khoa; tràn dịch màng phổi chảy tự do ở 9 bệnh nhân (22%), đa nang ở 24 bệnh nhân (58,5%) và có tổ chức ở 8 bệnh nhân (19,5%). Nội soi màng phổi bằng phương pháp nội khoa được coi là thành công mà không cần can thiệp thêm ở 35 trong số 41 bệnh nhân (85,4%): tất cả 9 bệnh nhân có dịch chảy tự do, 22 trong số 24 bệnh nhân bị tràn dịch màng phổi đa nang (91,7%) và chỉ 4 trong số 8 bệnh nhân bị tràn dịch có tổ chức (50%). KẾT LUẬN Nghiên cứu của chúng tôi xác nhận rằng tràn dịch màng phổi đa nang có thể được điều trị an toàn và thành công bằng nội soi màng phổi bằng phương pháp nội khoa trong khi tràn dịch màng phổi có tổ chức có thể kháng với việc dẫn lưu bằng nội soi màng phổi bằng phương pháp nội khoa, đòi hỏi phải phẫu thuật nội soi lồng ngực hỗ trợ video hoặc cắt bỏ màng phổi phẫu thuật mở; trong nhóm bệnh nhân này, sự hiện diện của các 'túi' riêng biệt không liên quan rõ ràng với nhau thường dẫn đến phương pháp phẫu thuật."} +{"idx": 1990138, "en": "Thymic carcinoids are a rare entity that may be associated with endocrine diseases like Cushing's syndrome or multiple endocrine neoplasia syndrome type I (MEN1). These tumors represent 4% of anterior mediastinal tumors and are characterized by their very aggressive behavior. We present the case of a patient with a previous MEN 1 diagnosis in whom, during the follow up of his disease, a thoracic image compatible with thymic carcinoid was detected. After an extended thymectomy that included peri-thymic fat resection, the clinical diagnosis was confirmed. A follow up examination 14 months later revealed a suspicious lesion that suggested local recurrence, therefore the patient was reoperated on. The pathology report of this surgery indicated post-radiation fibrosis. Likewise, we present a review of the current diagnostic and therapeutic management of patients with MEN1 syndrome who are diagnosed with thymic carcinoid.", "gemini": "Ung thư biểu mô tuyến ức là một thực thể hiếm có thể liên quan đến các bệnh nội tiết như hội chứng Cushing hoặc hội chứng tân sinh nội tiết đa dạng loại I (MEN1). Những khối u này chiếm 4% khối u trung thất trước và được đặc trưng bởi hành vi rất hung hăng của chúng. Chúng tôi trình bày trường hợp của một bệnh nhân được chẩn đoán MEN 1 trước đó, trong quá trình theo dõi bệnh của mình, đã phát hiện ra một hình ảnh trên ngực tương thích với ung thư biểu mô tuyến ức. Sau khi phẫu thuật cắt tuyến ức mở rộng bao gồm cắt bỏ mỡ quanh tuyến ức, chẩn đoán lâm sàng đã được xác nhận. Một cuộc kiểm tra tiếp theo 14 tháng sau cho thấy một tổn thương đáng ngờ gợi ý tái phát tại chỗ, do đó bệnh nhân đã được phẫu thuật lại. Báo cáo giải phẫu bệnh của cuộc phẫu thuật này cho thấy xơ hóa sau xạ trị. Tương tự như vậy, chúng tôi trình bày một đánh giá về chẩn đoán và điều trị hiện tại của những bệnh nhân mắc hội chứng MEN1 được chẩn đoán mắc ung thư biểu mô tuyến ức."} +{"idx": 1990139, "en": "BACKGROUND/AIMS It was the aim of this study to determine if intraoperative digital dilatation of the closed internal cervical ostium affects the healing of the uterine scar after a cesarean section (CS). METHODS In this randomized, prospective study, 94 term pregnant patients who elected to have a CS were assigned to two groups: intraoperative digital dilatation of the internal cervical ostium (group I, n = 48) or no dilatation (group II, n = 46). Vaginal ultrasound was used to check the integrity of the cesarean scar at the uterine incision site 40-42 days after the operation. We calculated the healing ratio based on ultrasonographic results and compared the groups as to the amount of incomplete healing. RESULTS The frequency of abnormal scarring was significantly higher in group II (14.3 vs. 40.6%; p < 0.05). The mean healing ratio was significantly higher in group I (0.95 ± 0.12) than in group II (0.87 ± 0.1; p < 0.05). In the multiple logistic regression model, digital dilatation remained the most significant factor affecting abnormal scar formation (odds ratio = 7.7). CONCLUSION Digital dilatation of a closed cervix in elective CS might lower the incidence of abnormal scarring of the uterine incision.", "gemini": "MỤC TIÊU NGHIÊN CỨU Mục đích của nghiên cứu này là xác định xem việc nong mở kỹ thuật số nội soi cổ tử cung trong lúc mổ có ảnh hưởng đến quá trình lành sẹo tử cung sau khi sinh mổ (CS) hay không. PHƯƠNG PHÁP Trong nghiên cứu này, 94 bệnh nhân mang thai đủ tháng đã chọn sinh mổ được chia thành hai nhóm: nhóm nong mở kỹ thuật số nội soi cổ tử cung trong lúc mổ (nhóm I, n = 48) hoặc không nong mở (nhóm II, n = 46). Siêu âm qua ngả âm đạo được sử dụng để kiểm tra tính toàn vẹn của sẹo mổ tại vị trí rạch tử cung 40-42 ngày sau phẫu thuật. Chúng tôi đã tính toán tỷ lệ lành dựa trên kết quả siêu âm và so sánh các nhóm về mức độ lành không hoàn toàn. KẾT QUẢ Tỷ lệ sẹo bất thường cao hơn đáng kể ở nhóm II (14,3 so với 40,6%; p < 0,05). Tỷ lệ lành trung bình cao hơn đáng kể ở nhóm I (0,95 ± 0,12) so với nhóm II (0,87 ± 0,1; p < 0,05). Trong mô hình hồi quy logistic đa biến, nong mở kỹ thuật số vẫn là yếu tố quan trọng nhất ảnh hưởng đến sự hình thành sẹo bất thường (tỷ lệ chênh lệch = 7,7). KẾT LUẬN Việc nong mở kỹ thuật số cổ tử cung khép kín trong sinh mổ theo yêu cầu có thể làm giảm tỷ lệ sẹo bất thường ở vết rạch tử cung."} +{"idx": 1990140, "en": "Omega-3 fatty acids have been proposed as an adjuvant treatment option in psychiatric disorders. Given their other health benefits and their relative lack of toxicity, teratogenicity and side effects, they may be particularly useful in children and in females of child-bearing age, especially during pregnancy and postpartum. A comprehensive mechanistic understanding of their effects is needed. Here we report translational studies demonstrating the phenotypic normalization and gene expression effects of dietary omega-3 fatty acids, specifically docosahexaenoic acid (DHA), in a stress-reactive knockout mouse model of bipolar disorder and co-morbid alcoholism, using a bioinformatic convergent functional genomics approach integrating animal model and human data to prioritize disease-relevant genes. Additionally, to validate at a behavioral level the novel observed effects on decreasing alcohol consumption, we also tested the effects of DHA in an independent animal model, alcohol-preferring (P) rats, a well-established animal model of alcoholism. Our studies uncover sex differences, brain region-specific effects and blood biomarkers that may underpin the effects of DHA. Of note, DHA modulates some of the same genes targeted by current psychotropic medications, as well as increases myelin-related gene expression. Myelin-related gene expression decrease is a common, if nonspecific, denominator of neuropsychiatric disorders. In conclusion, our work supports the potential utility of omega-3 fatty acids, specifically DHA, for a spectrum of psychiatric disorders such as stress disorders, bipolar disorder, alcoholism and beyond.", "gemini": "Axit béo Omega-3 đã được đề xuất như một lựa chọn điều trị bổ trợ trong các rối loạn tâm thần. Với những lợi ích sức khỏe khác và tương đối không độc hại, gây quái thai và tác dụng phụ, chúng có thể đặc biệt hữu ích ở trẻ em và phụ nữ trong độ tuổi sinh đẻ, đặc biệt là trong thời kỳ mang thai và sau sinh. Cần có sự hiểu biết toàn diện về cơ chế tác dụng của chúng. Ở đây chúng tôi báo cáo các nghiên cứu chuyển dịch chứng minh sự bình thường hóa kiểu hình và tác dụng biểu hiện gen của axit béo omega-3 trong chế độ ăn uống, đặc biệt là axit docosahexaenoic (DHA), trong một mô hình chuột loại trừ phản ứng căng thẳng của rối loạn lưỡng cực và nghiện rượu đồng mắc, sử dụng phương pháp tiếp cận giải trình tự gen chức năng hội tụ sinh học tích hợp mô hình động vật và dữ liệu của con người để ưu tiên các gen liên quan đến bệnh. Ngoài ra, để xác nhận ở cấp độ hành vi các tác dụng mới được quan sát thấy về việc giảm tiêu thụ rượu, chúng tôi cũng đã thử nghiệm tác dụng của DHA trên một mô hình động vật độc lập, chuột thích rượu (P), một mô hình động vật nghiện rượu đã được thiết lập tốt. Các nghiên cứu của chúng tôi phát hiện ra sự khác biệt về giới tính, tác dụng đặc hiệu theo vùng não và các dấu ấn sinh học trong máu có thể hỗ trợ cho các tác dụng của DHA. Lưu ý rằng DHA điều chỉnh một số gen tương tự như các loại thuốc hướng thần hiện tại, cũng như làm tăng biểu hiện gen liên quan đến myelin. Giảm biểu hiện gen liên quan đến myelin là một mẫu số chung, nếu không muốn nói là không đặc hiệu, của các rối loạn thần kinh. Tóm lại, công trình của chúng tôi hỗ trợ tiềm năng hữu ích của axit béo omega-3, đặc biệt là DHA, đối với một loạt các rối loạn tâm thần như rối loạn căng thẳng, rối loạn lưỡng cực, nghiện rượu và hơn thế nữa."} +{"idx": 1990141, "en": "A 74-year-old woman with left hemiparesis due to a previous stroke presented with the sudden development of cognitive impairment and episodic complex visual hallucinations as manifestations of nonconvulsive status epilepticus (NCSE). Neuropsychological tests performed during NCSE demonstrated deficits encompassing several cognitive domains. The short-lasting complex visual hallucinations were stereotypical, vivid, and accompanied by intense fear. The seizure activity in the right temporo-parieto-occipital region, as documented by SISCOM and electroencephalogram (EEG) recording, was thought to be due to an unusual clinical presentation of NCSE. Subcortical cerebromalacia likely blocked the propagation route, restricting the ictal activities within this area.", "gemini": "Một phụ nữ 74 tuổi bị liệt nửa người bên trái do đột quỵ trước đó đã xuất hiện tình trạng suy giảm nhận thức đột ngột và ảo giác thị giác phức tạp từng cơn như biểu hiện của trạng thái động kinh không co giật (NCSE). Các xét nghiệm tâm lý thần kinh được thực hiện trong NCSE cho thấy các khiếm khuyết bao gồm một số lĩnh vực nhận thức. Các ảo giác thị giác phức tạp kéo dài trong thời gian ngắn là rập khuôn, sống động và kèm theo nỗi sợ hãi dữ dội. Hoạt động co giật ở vùng thái dương - đỉnh - chẩm bên phải, như được ghi lại bằng SISCOM và điện não đồ (EEG), được cho là do biểu hiện lâm sàng bất thường của NCSE. Viêm mạch não bán cấp dưới vỏ có khả năng chặn đường lan truyền, hạn chế hoạt động động kinh trong khu vực này."} +{"idx": 1990142, "en": "OBJECTIVE To review cost-of-illness studies (COI) and economic evaluations (EE) conducted for medically unexplained symptoms and to analyze their methods and results. METHODS We searched the databases PubMed, PsycINFO and National Health Service Economic Evaluations Database of the University of York. Cost data were inflated to 2006 using country-specific gross domestic product inflators and converted to 2006 USD purchasing power parities. RESULTS We identified 5 COI and 8 EE, of which 6 were cost-minimization analyses and 2 were cost-effectiveness analyses. All studies used patient level data collected between 1980 and 2004 and were predominantly conducted in the USA (n = 10). COI found annual excess health care costs of somatizing patients between 432 and 5,353 USD in 2006 values. Indirect costs were estimated by only one EE and added up to about 18,000 USD per year. In EE, educational interventions for physicians as well as cognitive-behavioral therapy approaches for patients were evaluated. For both types of interventions, effectiveness was either shown within EE or by previous studies. Most EE found (often insignificant) cost reductions resulting from the interventions, but only two studies explicitly combined changes in costs with data on effectiveness to cost-effectiveness ratios (ratio of additional costs to additional effects). CONCLUSIONS Medically unexplained symptoms cause relevant annual excess costs in health care that are comparable to mental health problems like depression or anxiety disorders and which may be reduced by interventions targeting physicians as well as patients. More extensive research on indirect costs and cost-effectiveness is needed.", "gemini": "MỤC TIÊU Xem xét các nghiên cứu về chi phí bệnh tật (COI) và đánh giá kinh tế (EE) được tiến hành đối với các triệu chứng không giải thích được về mặt y học và phân tích các phương pháp và kết quả của chúng. PHƯƠNG PHÁP Chúng tôi đã tìm kiếm các cơ sở dữ liệu PubMed, PsycINFO và Cơ sở dữ liệu đánh giá kinh tế dịch vụ y tế quốc gia của Đại học York. Dữ liệu chi phí được điều chỉnh theo giá năm 2006 bằng cách sử dụng các công cụ điều chỉnh tổng sản phẩm quốc nội theo từng quốc gia và chuyển đổi sang sức mua tương đương năm 2006 bằng đô la Mỹ. KẾT QUẢ Chúng tôi đã xác định được 5 COI và 8 EE, trong đó 6 COI là phân tích chi phí tối thiểu và 2 COI là phân tích hiệu quả chi phí. Tất cả các nghiên cứu đều sử dụng dữ liệu cấp độ bệnh nhân được thu thập từ năm 1980 đến năm 2004 và chủ yếu được tiến hành tại Hoa Kỳ (n = 10). COI phát hiện ra chi phí chăm sóc sức khỏe vượt mức hàng năm của bệnh nhân có triệu chứng cơ thể hóa từ 432 đến 5.353 đô la Mỹ theo giá trị năm 2006. Chi phí gián tiếp chỉ được ước tính bởi một EE và lên tới khoảng 18.000 đô la Mỹ mỗi năm. Trong EE, các can thiệp giáo dục cho bác sĩ cũng như các phương pháp trị liệu nhận thức hành vi cho bệnh nhân đã được đánh giá. Đối với cả hai loại can thiệp, hiệu quả đã được thể hiện trong EE hoặc bởi các nghiên cứu trước đó. Hầu hết các EE đều phát hiện ra (thường không đáng kể) việc giảm chi phí do các can thiệp mang lại, nhưng chỉ có hai nghiên cứu kết hợp rõ ràng những thay đổi về chi phí với dữ liệu về hiệu quả theo tỷ lệ hiệu quả chi phí (tỷ lệ chi phí bổ sung so với hiệu ứng bổ sung). KẾT LUẬN Các triệu chứng không giải thích được về mặt y học gây ra chi phí vượt mức hàng năm có liên quan trong chăm sóc sức khỏe, tương đương với các vấn đề sức khỏe tâm thần như trầm cảm hoặc rối loạn lo âu và có thể giảm được bằng các can thiệp nhắm vào cả bác sĩ và bệnh nhân. Cần có nhiều nghiên cứu sâu hơn về chi phí gián tiếp và hiệu quả chi phí."} +{"idx": 1990143, "en": "While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need for domestic dogs, we investigated sequence data provided by the published 7.6X dog genome for novel short tandem repeat markers that met our criteria for sensitivity, stability, robustness, polymorphic information content, and ease of scoring. Fifteen unlinked tetranucleotide repeat markers were selected from a pool of 3113 candidate markers and assembled with a sex-linked marker into a multiplex capable of generating a full profile with as little as 60pg of nuclear DNA. An accompanying allelic ladder was assembled and sequenced to obtain detailed repeat motif data. Validation was carried out according to SWGDAM guidelines, and the DogFiler panel has been integrated into forensic casework and accepted in courts across the U.S. Applying various formulae for calculating random match probabilities for inbred populations, estimates for this panel of markers have proven to be comparable to those obtained in human forensic genetics. The DogFiler panel and the associated allelic ladder represent the first published non-human profiling system to fully address all SWGDAM recommendations.", "gemini": "Trong khi việc phân tích DNA của con người là trọng tâm của các nỗ lực hợp tác quy mô lớn, thì việc phân tích DNA pháp y không phải của con người lại không được hưởng lợi từ các nguồn tài trợ và sự phối hợp nỗ lực tương tự. Do đó, việc phát triển các bảng đánh dấu chuẩn, thang bậc alen và dữ liệu trình tự đặc hiệu alen tương đương với những dữ liệu đã thiết lập cho di truyền pháp y của con người đã bị tụt hậu. Để đáp ứng nhu cầu đó đối với chó nhà, chúng tôi đã nghiên cứu dữ liệu trình tự do bộ gen chó 7,6X đã công bố để tìm các dấu hiệu lặp lại tándem ngắn mới đáp ứng các tiêu chí của chúng tôi về độ nhạy, độ ổn định, độ mạnh, nội dung thông tin đa hình và dễ chấm điểm. Mười lăm dấu hiệu lặp lại tứ nucleotide không liên kết đã được chọn từ một nhóm 3113 dấu hiệu ứng viên và lắp ráp với một dấu hiệu liên kết giới tính thành một đa hợp có khả năng tạo ra một hồ sơ đầy đủ chỉ với 60pg DNA hạt nhân. Một thang bậc alen đi kèm đã được lắp ráp và giải trình tự để có được dữ liệu mô típ lặp lại chi tiết. Việc xác nhận được thực hiện theo hướng dẫn của SWGDAM và bảng DogFiler đã được tích hợp vào công tác pháp y và được chấp nhận tại các tòa án trên khắp Hoa Kỳ. Áp dụng các công thức khác nhau để tính xác suất trùng khớp ngẫu nhiên cho các quần thể cận huyết, ước tính cho bảng các dấu hiệu này đã được chứng minh là tương đương với những ước tính thu được trong di truyền pháp y của con người. Bảng DogFiler và thang bậc alen liên quan đại diện cho hệ thống lập hồ sơ không phải của con người đầu tiên được công bố để giải quyết đầy đủ tất cả các khuyến nghị của SWGDAM."} diff --git a/retranslated_vi_pubmed/chunk_199/error.json b/retranslated_vi_pubmed/chunk_199/error.json new file mode 100644 index 0000000000000000000000000000000000000000..033b60d85cf5ddd21e4162d78d1dcd8dccf8a3e9 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_199/error.json @@ -0,0 +1,5 @@ +[ + 1990099, + 1990100, + 1990101 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_2/completed.json b/retranslated_vi_pubmed/chunk_2/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..ff6fbee873f651b653595d0c57121affe3820112 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_2/completed.json @@ -0,0 +1,9999 @@ +[ + 20000, + 20001, + 20002, + 20003, + 20004, + 20005, + 20006, + 20007, + 20008, + 20009, + 20010, + 20011, + 20012, + 20013, + 20014, + 20015, + 20016, + 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treatment options in patients with advanced EGFR-mutant non-small cell lung cancer. Eventual resistance develops with multiple mechanisms identifiable both upon repeat biopsy and in plasma circulating tumor DNA. The T790M gatekeeper mutation is responsible for almost 60% of cases. A number of third-generation TKIs are in clinical development, and osimertinib has been approved by the US Food and Drug Administration for the treatment of patients with EGFR T790M mutant lung cancer after failure of initial EGFR kinase therapy. Resistance mechanisms are being identified to these novel agents, and the treatment landscape of EGFR-mutant lung cancer continues to evolve. The sequence of EGFR TKIs may change in the future and combination therapies targeting resistance appear highly promising.", "gemini": "Các đột biến tăng độ nhạy ở thụ thể yếu tố tăng trưởng biểu bì (EGFR) có thể dự đoán đáp ứng với thuốc ức chế tyrosine kinase EGFR (TKI) và cả TKI thế hệ đầu tiên và thế hệ thứ hai đều có sẵn như là các lựa chọn điều trị一线 ở những bệnh nhân ung thư phổi không phải tế bào nhỏ đột biến EGFR tiến triển. Cuối cùng, tình trạng kháng thuốc phát triển với nhiều cơ chế có thể xác định được cả khi sinh thiết lại và trong DNA khối u lưu hành trong huyết tương. Đột biến gác cổng T790M chịu trách nhiệm cho gần 60% các trường hợp. Một số TKI thế hệ thứ ba đang trong quá trình phát triển lâm sàng và osimertinib đã được Cục Quản lý Thực phẩm và Dược phẩm Hoa Kỳ chấp thuận để điều trị bệnh nhân ung thư phổi đột biến EGFR T790M sau khi thất bại của liệu pháp kinase EGFR ban đầu. Các cơ chế kháng thuốc đang được xác định đối với các tác nhân mới này và bối cảnh điều trị ung thư phổi đột biến EGFR tiếp tục phát triển. Trình tự TKI EGFR có thể thay đổi trong tương lai và các liệu pháp kết hợp nhắm vào tình trạng kháng thuốc có vẻ rất hứa hẹn."} +{"idx": 2000001, "en": "Bacterial obligate intracellular parasites are clinically significant animal and human pathogens. Central to the biology of these organisms is their level of adaptation to intracellular replication niches associated with physicochemical and nutritional constraints. While most bacterial pathogens can adapt to a wide range of environments, severe niche restriction-an inability to thrive in diverse environments-is a hallmark of bacterial obligate intracellular parasites. Herein the physicochemical and nutritional factors underlying the physiological basis for niche restriction in the zoonotic bacterial obligate intracellular parasite and Q fever agent Coxiella burnetii are characterized. Additionally, these factors are reviewed in the context of C. burnetii evolution and continued (patho) adaptation. C. burnetii replication was strictly dependent on a combination of moderately acidic pH, reduced oxygen tension, and presence of carbon dioxide. Of macronutrients, amino acids alone support replication under physicochemically favorable conditions. In addition to utilizing gluconeogenic substrates for replication, C. burnetii can also utilize glucose to generate biomass. A mutant with a disruption in the gene pckA, encoding phosphoenolpyruvate carboxykinase (PEPCK), the first committed step in gluconeogenesis, could be complemented chemically by the addition of glucose. Disruption of pckA resulted in a moderate glucose-dependent growth defect during infection of cultured host cells. Although, C. burnetii has the theoretical capacity to synthesize essential core metabolites via glycolysis and gluconeogenesis, amino acid auxotrophy essentially restricts C. burnetii replication to a niche providing ample access to amino acids. Overall, the described combination of physiochemical and nutritional growth requirements are strong indicators for why C. burnetii favors an acidified phagolysosome-derived vacuole in respiring tissue for replication.", "gemini": "Ký sinh trùng nội bào bắt buộc của vi khuẩn là các tác nhân gây bệnh có ý nghĩa lâm sàng ở động vật và người. Điểm trung tâm trong sinh học của những sinh vật này là mức độ thích nghi của chúng với các hốc nhân bản nội bào liên quan đến các ràng buộc vật lý hóa học và dinh dưỡng. Trong khi hầu hết các tác nhân gây bệnh do vi khuẩn có thể thích nghi với nhiều môi trường khác nhau, thì sự hạn chế hốc nghiêm ngặt - không có khả năng phát triển mạnh trong các môi trường đa dạng - là một đặc điểm của ký sinh trùng nội bào bắt buộc của vi khuẩn. Ở đây, các yếu tố vật lý hóa học và dinh dưỡng là cơ sở sinh lý cho sự hạn chế hốc ở ký sinh trùng nội bào bắt buộc của vi khuẩn động vật và tác nhân gây sốt Q Coxiella burnetii được đặc trưng. Ngoài ra, các yếu tố này được xem xét trong bối cảnh tiến hóa của C. burnetii và sự thích nghi (bệnh lý) liên tục. Sự sao chép của C. burnetii phụ thuộc chặt chẽ vào sự kết hợp của pH hơi axit, độ căng oxy giảm và sự có mặt của carbon dioxide. Trong số các chất dinh dưỡng đa lượng, chỉ có axit amin hỗ trợ sự sao chép trong các điều kiện thuận lợi về mặt vật lý hóa học. Ngoài việc sử dụng các chất nền sinh đường để sao chép, C. burnetii cũng có thể sử dụng glucose để tạo ra sinh khối. Một đột biến làm gián đoạn gen pckA, mã hóa phosphoenolpyruvate carboxykinase (PEPCK), bước đầu tiên được cam kết trong quá trình sinh đường, có thể được bổ sung hóa học bằng cách bổ sung glucose. Sự gián đoạn của pckA dẫn đến khuyết tật tăng trưởng phụ thuộc glucose vừa phải trong quá trình nhiễm trùng các tế bào vật chủ được nuôi cấy. Mặc dù vậy, C. burnetii có khả năng lý thuyết để tổng hợp các chất chuyển hóa cốt lõi thiết yếu thông qua quá trình đường phân và sinh đường, nhưng sự thiếu hụt axit amin về cơ bản hạn chế sự sao chép của C. burnetii thành một hốc cung cấp đầy đủ axit amin. Nhìn chung, sự kết hợp được mô tả của các yêu cầu tăng trưởng vật lý hóa học và dinh dưỡng là những chỉ số mạnh mẽ cho lý do tại sao C. burnetii thích một không bào có nguồn gốc từ phagolysosome có tính axit trong mô hô hấp để sao chép."} +{"idx": 2000002, "en": "Continuous stage conversion and swift changes in the antigenic repertoire in response to acquired immunity are hallmarks of complex eukaryotic pathogens, including Plasmodium species, the causative agents of malaria. Efficient elimination of Plasmodium liver stages prior to blood infection is one of the most promising malaria vaccine strategies. Here, we describe different genetically arrested parasites (GAPs) that have been engineered in Plasmodium berghei, P. yoelii and P. falciparum and compare their vaccine potential. A better understanding of the immunological mechanisms of prime and boost by arrested sporozoites and experimental strategies to enhance vaccine efficacy by further engineering existing GAPs into a more immunogenic form hold promise for continuous improvements of GAP-based vaccines. A critical hurdle for vaccines that elicit long-lasting protection against malaria, such as GAPs, is safety and efficacy in vulnerable populations. Vaccine research should focus on solutions toward turning malaria into a vaccine-preventable disease, which would offer an exciting new path of malaria control.", "gemini": "Sự chuyển đổi giai đoạn liên tục và những thay đổi nhanh chóng trong thành phần kháng nguyên để đáp ứng với khả năng miễn dịch mắc phải là những đặc điểm của các tác nhân gây bệnh eukaryote phức tạp, bao gồm các loài Plasmodium, tác nhân gây bệnh sốt rét. Loại bỏ hiệu quả các giai đoạn gan của Plasmodium trước khi nhiễm trùng máu là một trong những chiến lược vắc-xin sốt rét hứa hẹn nhất. Ở đây, chúng tôi mô tả các ký sinh trùng bị bắt giữ về mặt di truyền (GAP) khác nhau đã được thiết kế trong Plasmodium berghei, P. yoelii và P. falciparum và so sánh tiềm năng vắc-xin của chúng. Hiểu rõ hơn về các cơ chế miễn dịch của vắc-xin mồi và tăng cường bằng các sporozoit bị bắt giữ và các chiến lược thử nghiệm để tăng cường hiệu quả của vắc-xin bằng cách thiết kế thêm các GAP hiện có thành dạng sinh miễn dịch hơn hứa hẹn cải tiến liên tục các vắc-xin dựa trên GAP. Một rào cản quan trọng đối với các vắc-xin tạo ra khả năng bảo vệ lâu dài chống lại bệnh sốt rét, chẳng hạn như GAP, là tính an toàn và hiệu quả ở những đối tượng dễ bị tổn thương. Nghiên cứu vắc-xin nên tập trung vào các giải pháp hướng đến mục tiêu biến bệnh sốt rét thành bệnh có thể phòng ngừa bằng vắc-xin, điều này sẽ mở ra một con đường mới thú vị để kiểm soát bệnh sốt rét."} +{"idx": 2000003, "en": "New biomarkers are needed for monitoring the effectiveness of treatment for visceral leishmaniasis (VL). They might also improve the detection of the asymptomatic population in Leishmania-endemic areas. This paper examines the IL-2, IFN-γ, IFN-γ-induced protein 10 (IP-10), and monokine-induced-by-IFN-γ (MIG) levels in whole blood-stimulated in vitro with soluble Leishmania antigen (SLA)-taken from asymptomatic individuals and patients treated for VL living in a post-outbreak (Leishmania infantum) area in Spain, and in an endemic (Leishmania donovani) area of Bangladesh. IP-10 was found to be an accurate global marker of asymptomatic subjects with positive cellular/humoral tests, while MIG was found to be a better marker of contact with L. donovani than IL-2 but no for those with L. infantum. Determining IP-10, MIG, and IFN-γ levels proved useful in monitoring the cellular immune response following treatment for active disease caused by L. infantum.", "gemini": "Cần có các dấu ấn sinh học mới để theo dõi hiệu quả điều trị bệnh kala-azar nội tạng (VL). Chúng cũng có thể cải thiện việc phát hiện dân số không có triệu chứng ở các khu vực lưu hành Leishmania. Bài báo này xem xét mức IL-2, IFN-γ, protein 10 do IFN-γ gây ra (IP-10) và monokine do IFN-γ gây ra (MIG) trong toàn bộ máu được kích thích trong ống nghiệm với kháng nguyên Leishmania hòa tan (SLA) - lấy từ những người không có triệu chứng và bệnh nhân được điều trị VL sống ở khu vực hậu bùng phát (Leishmania infantum) ở Tây Ban Nha và ở khu vực lưu hành (Leishmania donovani) của Bangladesh. IP-10 được phát hiện là một dấu hiệu toàn cầu chính xác của những đối tượng không có triệu chứng với các xét nghiệm tế bào/dịch thể dương tính, trong khi MIG được phát hiện là dấu hiệu tiếp xúc với L. donovani tốt hơn IL-2 nhưng không đối với những người mắc L. infantum. Xác định mức IP-10, MIG và IFN-γ tỏ ra hữu ích trong việc theo dõi đáp ứng miễn dịch tế bào sau khi điều trị bệnh hoạt động do L. infantum gây ra."} +{"idx": 2000004, "en": "Bacteria have acquired multiple systems to expose proteins on their surface, release them in the extracellular environment or even inject them into a neighboring cell. Protein secretion has a high adaptive value and secreted proteins are implicated in many functions, which are often essential for bacterial fitness. Several secreted proteins or secretion machineries have been extensively studied as potential drug targets. It is therefore important to identify the secretion substrates, to understand how they are specifically recognized by the secretion machineries, and how transport through these machineries occurs. The purpose of this review is to provide an overview of the biochemical, genetic and imaging tools that have been developed to evaluate protein secretion in a qualitative or quantitative manner. After a brief overview of the different tools available, we will illustrate their advantages and limitations through a discussion of some of the current open questions related to protein secretion. We will start with the question of the identification of secreted proteins, which for many bacteria remains a critical initial step toward a better understanding of their interactions with the environment. We will then illustrate our toolbox by reporting how these tools have been applied to better understand how substrates are recognized by their cognate machinery, and how secretion proceeds. Finally, we will highlight recent approaches that aim at investigating secretion in real time, and in complex environments such as a tissue or an organism.", "gemini": "Vi khuẩn đã có được nhiều hệ thống để phơi bày protein trên bề mặt của chúng, giải phóng chúng vào môi trường ngoại bào hoặc thậm chí tiêm chúng vào tế bào lân cận. Quá trình tiết protein có giá trị thích nghi cao và các protein tiết ra có liên quan đến nhiều chức năng, thường thiết yếu cho sự thích nghi của vi khuẩn. Một số protein tiết ra hoặc máy móc tiết ra đã được nghiên cứu rộng rãi như các mục tiêu thuốc tiềm năng. Do đó, điều quan trọng là phải xác định các chất nền tiết ra, để hiểu cách chúng được các máy móc tiết ra nhận dạng cụ thể và cách vận chuyển qua các máy móc này diễn ra như thế nào. Mục đích của bài đánh giá này là cung cấp một cái nhìn tổng quan về các công cụ sinh hóa, di truyền và hình ảnh đã được phát triển để đánh giá quá trình tiết protein theo cách định tính hoặc định lượng. Sau một cái nhìn tổng quan ngắn gọn về các công cụ khác nhau có sẵn, chúng tôi sẽ minh họa những ưu điểm và hạn chế của chúng thông qua việc thảo luận về một số câu hỏi mở hiện tại liên quan đến quá trình tiết protein. Chúng tôi sẽ bắt đầu với câu hỏi về việc xác định các protein tiết ra, đối với nhiều loại vi khuẩn vẫn là một bước ban đầu quan trọng để hiểu rõ hơn về sự tương tác của chúng với môi trường. Sau đó, chúng tôi sẽ minh họa hộp công cụ của mình bằng cách báo cáo cách các công cụ này đã được áp dụng để hiểu rõ hơn về cách các chất nền được máy móc nhận dạng và quá trình tiết ra diễn ra như thế nào. Cuối cùng, chúng tôi sẽ nêu bật các phương pháp tiếp cận gần đây nhằm mục đích nghiên cứu quá trình tiết ra theo thời gian thực và trong các môi trường phức tạp như mô hoặc cơ thể."} +{"idx": 2000005, "en": "Knowledge of the transcriptional responses of vector-borne pathogens at the vector-pathogen interface is critical for understanding disease transmission. Borrelia (Borreliella) burgdorferi, the causative agent of Lyme disease in the United States, is transmitted by the bite of infected Ixodes sp. ticks. It is known that B. burgdorferi has altered patterns of gene expression during tick acquisition, persistence and transmission. Recently, we and others have discovered in vitro expression of RNAs found internal, overlapping, and antisense to annotated open reading frames in the B. burgdorferi genome. However, there is a lack of molecular genetic tools for B. burgdorferi for quantitative, strand-specific, comparative analysis of these transcripts in distinct environments such as the arthropod vector. To address this need, we have developed a dual luciferase reporter system to quantify B. burgdorferi promoter activities in a strand-specific manner. We demonstrate that constitutive expression of a B. burgdorferi codon-optimized Renilla reniformis luciferase gene (rluc ) allows normalization of the activity of a promoter of interest when fused to the B. burgdorferi codon-optimized Photinus pyralis luciferase gene (fluc) on the same plasmid. Using the well characterized, differentially regulated, promoters for flagellin (flaBp), outer surface protein A (ospAp) and outer surface protein C (ospCp), we document the efficacy of the dual luciferase system for quantitation of promoter activities during in vitro growth and in infected ticks. Cumulatively, the dual luciferase method outlined herein is the first dual reporter system for B. burgdorferi, providing a novel and highly versatile approach for strand-specific molecular genetic analyses.", "gemini": "Kiến thức về phản ứng phiên mã của các mầm bệnh do véc tơ truyền tại giao diện véc tơ-mầm bệnh rất quan trọng để hiểu về quá trình lây truyền bệnh. Borrelia (Borreliella) burgdorferi, tác nhân gây bệnh Lyme ở Hoa Kỳ, được truyền qua vết cắn của ve Ixodes sp. bị nhiễm bệnh. Người ta biết rằng B. burgdorferi đã thay đổi các kiểu biểu hiện gen trong quá trình tiếp nhận, tồn tại và lây truyền của ve. Gần đây, chúng tôi và những người khác đã phát hiện ra biểu hiện trong ống nghiệm của RNA được tìm thấy bên trong, chồng chéo và ngược nghĩa với các khung đọc mở được chú thích trong bộ gen B. burgdorferi. Tuy nhiên, vẫn thiếu các công cụ di truyền phân tử đối với B. burgdorferi để phân tích so sánh định lượng, theo mạch cụ thể của các bản sao này trong các môi trường riêng biệt như véc tơ chân đốt. Để giải quyết nhu cầu này, chúng tôi đã phát triển một hệ thống báo cáo luciferase kép để định lượng hoạt động của chất thúc đẩy B. burgdorferi theo cách cụ thể theo mạch. Chúng tôi chứng minh rằng biểu hiện cấu thành của gen luciferase Renilla reniformis được tối ưu hóa theo mã di truyền B. burgdorferi (rluc) cho phép chuẩn hóa hoạt động của chất thúc đẩy được quan tâm khi hợp nhất với gen luciferase Photinus pyralis được tối ưu hóa theo mã di truyền B. burgdorferi (fluc) trên cùng một plasmid. Sử dụng các chất thúc đẩy được điều chỉnh khác biệt, được đặc trưng tốt cho flagellin (flaBp), protein bề mặt ngoài A (ospAp) và protein bề mặt ngoài C (ospCp), chúng tôi ghi lại hiệu quả của hệ thống luciferase kép để định lượng hoạt động của chất thúc đẩy trong quá trình phát triển trong ống nghiệm và trong ve bị nhiễm bệnh. Về mặt tích lũy, phương pháp luciferase kép được nêu ở đây là hệ thống báo cáo kép đầu tiên đối với B. burgdorferi, cung cấp một phương pháp tiếp cận mới và rất linh hoạt cho các phân tích di truyền phân tử cụ thể theo mạch."} +{"idx": 2000006, "en": "One strategy to develop the next generation of tuberculosis vaccines is to construct subunit vaccines based on T cell antigens. In this study, we have evaluated the vaccine potential of a fusion protein combining EsxB, EsxD, EsxG, EsxU, and EsxM of Mycobacterium tuberculosis (M. tb). This recombinant protein, named BM, was expressed in and purified from Escherichia coli. Immunization of C57BL/6 mice with purified BM protein formulated in Freund's incomplete adjuvant induced the production of Th1 cytokines (IFN-γ, TNF, and IL-2) and multifunctional CD4+ T cells. Vaccination of BALB/c mice with BM protein followed by intravenous challenge with Mycobacterium bovis BCG resulted in better levels of protection than the two leading antigens, Ag85A and PPE18. Taken together, these results indicate that BM is a protective antigen. Future studies to combine BM with other antigens and evaluate its effectiveness as a booster of BCG or as a therapeutic vaccine are warranted.", "gemini": "Một chiến lược để phát triển thế hệ vắc-xin chống lao tiếp theo là xây dựng các loại vắc-xin tiểu đơn vị dựa trên các kháng nguyên tế bào T. Trong nghiên cứu này, chúng tôi đã đánh giá tiềm năng vắc-xin của một protein tổng hợp kết hợp EsxB, EsxD, EsxG, EsxU và EsxM của Mycobacterium tuberculosis (M. tb). Protein tái tổ hợp này, có tên là BM, được biểu hiện và tinh chế từ Escherichia coli. Việc tiêm chủng protein BM tinh khiết cho chuột C57BL/6 được pha chế trong tá dược không hoàn chỉnh của Freund đã gây ra sự sản sinh các cytokine Th1 (IFN-γ, TNF và IL-2) và các tế bào CD4+ T đa chức năng. Việc tiêm vắc-xin protein BM cho chuột BALB/c tiếp theo là thử thách tiêm tĩnh mạch với Mycobacterium bovis BCG đã mang lại mức độ bảo vệ tốt hơn so với hai kháng nguyên hàng đầu, Ag85A và PPE18. Kết hợp lại, những kết quả này chỉ ra rằng BM là một kháng nguyên bảo vệ. Các nghiên cứu trong tương lai để kết hợp BM với các kháng nguyên khác và đánh giá hiệu quả của nó như một chất tăng cường BCG hoặc như một vắc-xin điều trị là cần thiết."} +{"idx": 2000007, "en": "Ticks are vectors of pathogens affecting human and animal health worldwide. Nevertheless, the ecological and evolutionary interactions between ticks, hosts, and pathogens are largely unknown. Here, we integrated a framework to evaluate the associations of the tick Ixodes ricinus with its hosts and environmental niches that impact pathogen circulation. The analysis of tick-hosts association suggested that mammals and lizards were the ancestral hosts of this tick species, and that a leap to Aves occurred around 120 M years ago. The signature of the environmental variables over the host's phylogeny revealed the existence of two clades of vertebrates diverging along a temperature and vegetation split. This is a robust proof that the tick probably experienced a colonization of new niches by adapting to a large set of new hosts, Aves. Interestingly, the colonization of Aves as hosts did not increase significantly the ecological niche of I. ricinus, but remarkably Aves are super-spreaders of pathogens. The disparate contribution of Aves to the tick-host-pathogen networks revealed that I. ricinus evolved to maximize habitat overlap with some hosts that are super-spreaders of pathogens. These results supported the hypothesis that large host networks are not a requirement of tick survival but pathogen circulation. The biological cost of tick adaptation to non-optimal environmental conditions might be balanced by molecular mechanisms triggered by the pathogens that we have only begun to understand.", "gemini": "Ve là vật trung gian truyền bệnh gây ảnh hưởng đến sức khỏe của con người và động vật trên toàn thế giới. Tuy nhiên, các tương tác sinh thái và tiến hóa giữa ve, vật chủ và mầm bệnh phần lớn vẫn chưa được biết rõ. Ở đây, chúng tôi đã tích hợp một khuôn khổ để đánh giá các mối liên quan của loài ve Ixodes ricinus với vật chủ và các hốc sinh thái có tác động đến sự lưu hành của mầm bệnh. Phân tích mối liên quan giữa ve và vật chủ cho thấy động vật có vú và thằn lằn là vật chủ tổ tiên của loài ve này và loài này đã nhảy sang loài Aves khoảng 120 triệu năm trước. Dấu hiệu của các biến số môi trường trên quá trình phát sinh loài của vật chủ đã tiết lộ sự tồn tại của hai nhánh động vật có xương sống phân kỳ theo sự phân chia nhiệt độ và thảm thực vật. Đây là một bằng chứng chắc chắn cho thấy loài ve có thể đã trải qua quá trình xâm chiếm các hốc sinh thái mới bằng cách thích nghi với một tập hợp lớn các vật chủ mới, Aves. Điều thú vị là việc xâm chiếm Aves làm vật chủ không làm tăng đáng kể hốc sinh thái của I. ricinus, nhưng đáng chú ý là Aves là loài siêu lây lan mầm bệnh. Sự đóng góp không đồng đều của Aves vào các mạng lưới ve-vật chủ-mầm bệnh cho thấy I. ricinus đã tiến hóa để tối đa hóa sự chồng lấn môi trường sống với một số vật chủ là loài siêu lây lan mầm bệnh. Những kết quả này ủng hộ giả thuyết rằng các mạng lưới vật chủ lớn không phải là yêu cầu để ve tồn tại mà là sự lưu hành của mầm bệnh. Chi phí sinh học của việc ve thích nghi với các điều kiện môi trường không tối ưu có thể được cân bằng bằng các cơ chế phân tử do các tác nhân gây bệnh kích hoạt mà chúng ta mới chỉ bắt đầu hiểu."} +{"idx": 2000008, "en": "Giardia is a highly prevalent, understudied protistan parasite causing significant diarrheal disease worldwide. Its life cycle consists of two stages: infectious cysts ingested from contaminated food or water sources, and motile trophozoites that colonize and attach to the gut epithelium, later encysting to form new cysts that are excreted into the environment. Current understanding of parasite physiology in the host is largely inferred from transcriptomic studies using Giardia grown axenically or in co-culture with mammalian cell lines. The dearth of information about the diversity of host-parasite interactions occurring within distinct regions of the gastrointestinal tract has been exacerbated by a lack of methods to directly and non-invasively interrogate disease progression and parasite physiology in live animal hosts. By visualizing Giardia infections in the mouse gastrointestinal tract using bioluminescent imaging (BLI) of tagged parasites, we recently showed that parasites colonize the gut in high-density foci. Encystation is initiated in these foci throughout the entire course of infection, yet how the physiology of parasites within high-density foci in the host gut differs from that of cells in laboratory culture is unclear. Here we use BLI to precisely select parasite samples from high-density foci in the proximal intestine to interrogate in vivo Giardia gene expression in the host. Relative to axenic culture, we noted significantly higher expression (>10-fold) of oxidative stress, membrane transporter, and metabolic and structural genes associated with encystation in the high-density foci. These differences in gene expression within parasite foci in the host may reflect physiological changes associated with high-density growth in localized regions of the gut. We also identified and verified six novel cyst-specific proteins, including new components of the cyst wall that were highly expressed in these foci. Our in vivo transcriptome data support an emerging view that parasites encyst early in localized regions in the gut, possibly as a consequence of nutrient limitation, and also impact local metabolism and physiology.", "gemini": "Giardia là một loại ký sinh trùng nguyên sinh có tỷ lệ lưu hành cao, ít được nghiên cứu gây ra bệnh tiêu chảy đáng kể trên toàn thế giới. Vòng đời của nó bao gồm hai giai đoạn: nang truyền nhiễm được ăn vào từ nguồn thức ăn hoặc nước bị ô nhiễm và các trophozoit vận động xâm chiếm và bám vào biểu mô ruột, sau đó tạo thành nang mới được thải ra môi trường. Hiểu biết hiện tại về sinh lý học của ký sinh trùng ở vật chủ phần lớn được suy ra từ các nghiên cứu phiên mã sử dụng Giardia được nuôi cấy đơn độc hoặc trong nuôi cấy chung với các dòng tế bào động vật có vú. Sự thiếu thông tin về tính đa dạng của các tương tác vật chủ-ký sinh trùng xảy ra trong các vùng riêng biệt của đường tiêu hóa đã trở nên trầm trọng hơn do thiếu các phương pháp để thẩm vấn trực tiếp và không xâm lấn về tiến triển của bệnh và sinh lý học của ký sinh trùng ở vật chủ động vật sống. Bằng cách hình dung các bệnh nhiễm Giardia trong đường tiêu hóa của chuột bằng cách sử dụng hình ảnh phát quang sinh học (BLI) của các ký sinh trùng được gắn thẻ, gần đây chúng tôi đã chỉ ra rằng các ký sinh trùng xâm chiếm ruột ở các ổ mật độ cao. Việc tạo nang được bắt đầu ở các ổ này trong suốt quá trình nhiễm trùng, nhưng cách thức sinh lý học của các ký sinh trùng trong các ổ mật độ cao trong ruột vật chủ khác với các tế bào trong nuôi cấy trong phòng thí nghiệm vẫn chưa rõ ràng. Ở đây, chúng tôi sử dụng BLI để lựa chọn chính xác các mẫu ký sinh trùng từ các ổ mật độ cao ở ruột gần để thẩm vấn biểu hiện gen của Giardia trong cơ thể vật chủ. So với nuôi cấy đơn độc, chúng tôi ghi nhận biểu hiện cao hơn đáng kể (>10 lần) của các gen liên quan đến stress oxy hóa, chất vận chuyển màng, chuyển hóa và cấu trúc liên quan đến việc tạo nang ở các ổ mật độ cao. Những khác biệt về biểu hiện gen trong các ổ ký sinh trùng ở vật chủ có thể phản ánh những thay đổi về sinh lý liên quan đến sự phát triển mật độ cao ở các vùng cục bộ của ruột. Chúng tôi cũng đã xác định và xác minh sáu protein đặc hiệu của nang mới, bao gồm các thành phần mới của thành nang có biểu hiện cao trong các ổ này. Dữ liệu phiên mã trong cơ thể sống của chúng tôi hỗ trợ cho quan điểm mới nổi rằng các ký sinh trùng tạo nang sớm ở các vùng cục bộ trong ruột, có thể là do thiếu chất dinh dưỡng và cũng ảnh hưởng đến quá trình trao đổi chất và sinh lý cục bộ."} +{"idx": 2000009, "en": "BACKGROUND This randomized, parallel-group, non-blinded study was designed to determine the role of fibular fixation in the treatment outcomes of combined distal tibia and fibula fractures. MATERIALS AND METHODS Sixty patients with distal tibial and fibular fractures were randomly divided in two groups of case and controls. In the case group, fibula was fixed prior to the fixation of tibia. In the control group, tibia was fixed without fibular fixation. Primary outcomes were varus-valgus angulation, anterior-posterior angulation, union and side effects. Follow-up visit and radiographs were taken 2 and 4 weeks as well as 3, 6 and 9 months after surgery. RESULTS During the follow-up, 11 out of 60 patients in case and control groups were excluded. We recruited 24 and 25 patients in the case and control group, respectively. Intramedullary nailing was used in 8 patients of case and 11 patients of control group. Plate and screw were used in 16 patients in the case and 14 patients in the control group. Varus/valgus and anterior-posterior angulation were not statistically significant between two groups (P ≥ 0.05). The frequency of tibial and fibula union after 1, 3, 6 and 9 months in case and controls groups were not statistically significant (P ≥ 0.05). The frequency of nonunion of tibia and fibula, infection and nerve injury in studied groups were not statistically significant (P ≥ 0.05). CONCLUSION We did not observe any significant improvement using fibular fixation in the treatment outcomes of tibia distal fractures.", "gemini": "BỐI CẢNH Nghiên cứu ngẫu nhiên, nhóm song song, không mù đôi này được thiết kế để xác định vai trò của cố định xương mác trong kết quả điều trị gãy xương chày xa và xương mác kết hợp. VẬT LIỆU VÀ PHƯƠNG PHÁP Sáu mươi bệnh nhân bị gãy xương chày xa và xương mác được chia ngẫu nhiên thành hai nhóm trường hợp và đối chứng. Ở nhóm trường hợp, xương mác được cố định trước khi cố định xương chày. Ở nhóm đối chứng, xương chày được cố định mà không cố định xương mác. Các kết cục chính là góc lệch ngoài-trong, góc lệch trước-sau, sự liền xương và tác dụng phụ. Chụp X-quang và thăm khám theo dõi được thực hiện sau 2 và 4 tuần cũng như sau 3, 6 và 9 tháng sau phẫu thuật. KẾT QUẢ Trong quá trình theo dõi, 11 trong số 60 bệnh nhân ở nhóm trường hợp và đối chứng đã được loại trừ. Chúng tôi đã tuyển dụng lần lượt 24 và 25 bệnh nhân vào nhóm trường hợp và nhóm đối chứng. Đối với 8 bệnh nhân trong nhóm trường hợp và 11 bệnh nhân trong nhóm đối chứng, có sử dụng nẹp tủy. Đối với 16 bệnh nhân trong nhóm trường hợp và 14 bệnh nhân trong nhóm đối chứng, có sử dụng vít và nẹp. Góc lệch ngoài/trong và góc lệch trước-sau không có ý nghĩa thống kê giữa hai nhóm (P ≥ 0,05). Tần suất liền xương chày và xương mác sau 1, 3, 6 và 9 tháng ở nhóm trường hợp và nhóm đối chứng không có ý nghĩa thống kê (P ≥ 0,05). Tần suất không liền xương chày và xương mác, nhiễm trùng và tổn thương thần kinh ở các nhóm nghiên cứu không có ý nghĩa thống kê (P ≥ 0,05). KẾT LUẬN Chúng tôi không quan sát thấy bất kỳ cải thiện đáng kể nào khi sử dụng cố định xương mác trong kết quả điều trị gãy xương chày xa."} +{"idx": 2000010, "en": "BACKGROUND No previous study exists to evaluate serum phosphorus (Ph) level as a predictor of the need to mechanical ventilation (MV). This study was designed to determine the predictive ability of admission serum Ph level on MV in patients admitted in Intensive Care Unit (ICU). MATERIALS AND METHODS This prospective study was conducted on 100 patients (>16 years old), admitted to our ICU over 1-year. Patients were classified into two groups according to the days of the need to MV. Group A: Patients who required equal or <5 days MV, and Group B: Patients who required more than 5 days of MV. We measured total serum Ph concentrations at the times of ICU admission, connecting to the ventilator and weaning from the ventilator. RESULTS There were significant differences between serum Ph concentration on admission to ICU (Group A: 3.39 ± 0.39 mg/dl, Group B: 2.89 ± 0.31 mg/dl, P < 0.001), at the time of connecting to ventilator (Group A: 2.49 ± 0.38 mg/dl, Group B: 2.25 ± 0.26 mg/dl, P = 0.004) and weaning from ventilator (Group A: 3.42 ± 0.33 mg/dl, Group B: 2.98 ± 0.34 mg/dl, P < 0.001) between two groups. Duration of ICU stay in Group A was 6.08 ± 1.48 days and in Group B was 15.35 ± 6.45, this difference was significant (P < 0.001). We found the best cut-off point of 3.07 for serum Ph concentration to predict the longer duration of MV. CONCLUSION According to the results of our study, hypophosphatemia may increase the need to MV. Therefore, monitoring serum Ph level is a good prognostic factor to predict the need to ventilation.", "gemini": "BỐI CẢNH Chưa có nghiên cứu trước đây nào đánh giá nồng độ phốt pho (Ph) trong huyết thanh như một yếu tố dự báo nhu cầu phải thở máy (MV). Nghiên cứu này được thiết kế để xác định khả năng dự đoán nồng độ Ph huyết thanh khi nhập viện đối với MV ở những bệnh nhân được đưa vào Khoa chăm sóc đặc biệt (ICU). VẬT LIỆU VÀ PHƯƠNG PHÁP Nghiên cứu triển vọng này được tiến hành trên 100 bệnh nhân (>16 tuổi) được đưa vào ICU của chúng tôi trong hơn 1 năm. Bệnh nhân được phân loại thành hai nhóm theo số ngày cần thở máy. Nhóm A: Bệnh nhân cần thở máy bằng hoặc dưới 5 ngày và Nhóm B: Bệnh nhân cần thở máy trên 5 ngày. Chúng tôi đã đo nồng độ Ph huyết thanh toàn phần tại thời điểm nhập viện ICU, kết nối với máy thở và cai máy thở. KẾT QUẢ Có sự khác biệt đáng kể giữa nồng độ Ph huyết thanh khi nhập viện ICU (Nhóm A: 3,39 ± 0,39 mg/dl, Nhóm B: 2,89 ± 0,31 mg/dl, P < 0,001), tại thời điểm kết nối với máy thở (Nhóm A: 2,49 ± 0,38 mg/dl, Nhóm B: 2,25 ± 0,26 mg/dl, P = 0,004) và cai máy thở (Nhóm A: 3,42 ± 0,33 mg/dl, Nhóm B: 2,98 ± 0,34 mg/dl, P < 0,001) giữa hai nhóm. Thời gian nằm ICU ở Nhóm A là 6,08 ± 1,48 ngày và ở Nhóm B là 15,35 ± 6,45, sự khác biệt này là có ý nghĩa (P < 0,001). Chúng tôi tìm thấy điểm cắt tối ưu là 3,07 đối với nồng độ Ph huyết thanh để dự đoán thời gian thở máy dài hơn. KẾT LUẬN Theo kết quả nghiên cứu của chúng tôi, tình trạng hạ phosphat máu có thể làm tăng nhu cầu thở máy. Do đó, theo dõi nồng độ Ph huyết thanh là một yếu tố tiên lượng tốt để dự đoán nhu cầu thở máy."} +{"idx": 2000011, "en": "OBJECTIVES To assess the optimal length of follow-up for patients undergoing both open and minimally invasive pyeloplasties to ensure prompt detection of a recurrent obstruction. There are no standard guidelines on ideal follow-up and imaging post-pediatric pyeloplasty currently. METHODS A retrospective chart review identified 264 patients (<18 years old) who underwent pyeloplasty for ureteropelvic junction obstruction between April 2002 and December 2014. Ultrasound was obtained every 3-4 months for the first year following pyeloplasty and thereafter at discretion of treating physician. Patient characteristics including symptoms and imaging were reviewed. RESULTS Of the 264 patients, 72% were male with mean age of 51 months and follow-up of 26.8 months. Approximately 73% followed up to 3 years. Fourteen patients (5.3%) had a recurrent obstruction. Among the failures, 85% were diagnosed and underwent successful redo pyeloplasty within 3 years. Six infants had a recurrence (43% of all unsuccessful surgeries) and were diagnosed within 3 years of the initial surgery. Patients undergoing a minimally invasive procedure were less likely to be followed for more than 3 years compared to an open procedure (p < 0.001). Patients with severe hydronephrosis preoperatively were followed longer (p = 0.031). Age at surgery and type of surgical approach (p < 0.01) were significant predictors of length of follow-up in a negative binomial regression. CONCLUSION Based on the results, a minimum of 3 years of follow-up is necessary to detect the majority of recurrent obstructions. Those patients who have higher than average lengths of follow-up tend to be younger and/or underwent an open surgical approach.", "gemini": "MỤC TIÊU Đánh giá thời gian theo dõi tối ưu cho những bệnh nhân trải qua cả phẫu thuật tạo hình bể thận mở và ít xâm lấn để đảm bảo phát hiện kịp thời tình trạng tắc nghẽn tái phát. Hiện tại, không có hướng dẫn chuẩn nào về theo dõi và chẩn đoán hình ảnh lý tưởng sau phẫu thuật tạo hình bể thận ở trẻ em. PHƯƠNG PHÁP Xem xét biểu đồ hồi cứu đã xác định được 264 bệnh nhân (<18 tuổi) đã trải qua phẫu thuật tạo hình bể thận do tắc nghẽn chỗ nối niệu quản-bể thận từ tháng 4 năm 2002 đến tháng 12 năm 2014. Siêu âm được thực hiện 3-4 tháng một lần trong năm đầu tiên sau phẫu thuật tạo hình bể thận và sau đó theo quyết định của bác sĩ điều trị. Các đặc điểm của bệnh nhân bao gồm các triệu chứng và hình ảnh đã được xem xét. KẾT QUẢ Trong số 264 bệnh nhân, 72% là nam giới với độ tuổi trung bình là 51 tháng và thời gian theo dõi là 26,8 tháng. Khoảng 73% theo dõi đến 3 năm. Mười bốn bệnh nhân (5,3%) bị tắc nghẽn tái phát. Trong số những ca thất bại, 85% được chẩn đoán và trải qua phẫu thuật tạo hình bể thận thành công trong vòng 3 năm. Sáu trẻ sơ sinh bị tái phát (chiếm 43% tổng số ca phẫu thuật không thành công) và được chẩn đoán trong vòng 3 năm kể từ ca phẫu thuật ban đầu. Những bệnh nhân trải qua một thủ thuật ít xâm lấn ít có khả năng được theo dõi trong hơn 3 năm so với một thủ thuật mở (p < 0,001). Những bệnh nhân bị giãn thận nặng trước phẫu thuật được theo dõi lâu hơn (p = 0,031). Tuổi phẫu thuật và loại phương pháp phẫu thuật (p < 0,01) là những yếu tố dự báo đáng kể về thời gian theo dõi trong hồi quy nhị thức âm. KẾT LUẬN Dựa trên kết quả, cần tối thiểu 3 năm theo dõi để phát hiện phần lớn các trường hợp tắc nghẽn tái phát. Những bệnh nhân có thời gian theo dõi dài hơn mức trung bình có xu hướng trẻ hơn và/hoặc trải qua phương pháp phẫu thuật mở."} +{"idx": 2000012, "en": "Feeding difficulties such as dysphagia, coughing, choking, or vomiting during meals, slow eating, oral aversion, food refusal, and stressful mealtimes are common in children with repaired esophageal atresia (EA) and the reasons for this are often multifactorial. The aim of this review is to describe the possible underlying mechanisms contributing to feeding difficulties in patients with EA and approaches to management. Underlying mechanisms for these feeding difficulties include esophageal dysphagia, oropharyngeal dysphagia and aspiration, and aversions related to prolonged gastrostomy tube feeding. The initial diagnostic evaluation for feeding difficulties in a patient with EA may involve an esophagram, videofluoroscopic imaging or fiberoptic endoscopic evaluation during swallowing, upper endoscopy with biopsies, pH-impedance testing, and/or esophageal motility studies. The main goal of management is to reduce the factors contributing to feeding difficulties and may include reducing esophageal stasis, maximizing reflux therapies, treating underlying lung disease, dilating strictures, and altering feeding methods, routes, or schedules.", "gemini": "Những khó khăn khi ăn như khó nuốt, ho, nghẹn hoặc nôn trong bữa ăn, ăn chậm, biếng ăn, từ chối thức ăn và thời gian ăn căng thẳng là phổ biến ở trẻ em bị teo thực quản (EA) đã được phục hồi và lý do cho điều này thường là đa yếu tố. Mục đích của bài đánh giá này là mô tả các cơ chế tiềm ẩn có thể góp phần gây ra khó khăn khi ăn ở những bệnh nhân bị EA và các cách tiếp cận để quản lý. Các cơ chế tiềm ẩn gây ra những khó khăn khi ăn này bao gồm khó nuốt thực quản, khó nuốt và hít sặc ở hầu họng và tình trạng biếng ăn liên quan đến việc nuôi ăn qua ống thông dạ dày kéo dài. Đánh giá chẩn đoán ban đầu về khó khăn khi ăn ở bệnh nhân bị EA có thể bao gồm chụp thực quản, chụp X-quang huỳnh quang hoặc đánh giá nội soi bằng sợi quang trong khi nuốt, nội soi trên có sinh thiết, thử nghiệm pH-trở kháng và/hoặc nghiên cứu nhu động thực quản. Mục tiêu chính của việc quản lý là giảm các yếu tố góp phần gây khó khăn khi ăn và có thể bao gồm giảm tình trạng ứ đọng thực quản, tối đa hóa liệu pháp trào ngược, điều trị bệnh phổi tiềm ẩn, nong giãn các chỗ hẹp và thay đổi phương pháp cho ăn, đường cho ăn hoặc lịch trình cho ăn."} +{"idx": 2000013, "en": "Esophageal dysmotility is almost universal after esophageal atresia (EA) repair and is mainly related to the developmental anomaly of the esophagus. Esophageal dysmotility is involved in the pathophysiology of numerous symptoms and comorbidities associated with EA such as gastroesophageal reflux disease, aspiration and respiratory complications, and symptoms of dysphagia and feeding disorders. High-resolution esophageal manometry (HREM) has facilitated the characterization of the dysmotility, but there is an incomplete correlation between symptoms and manometrical patterns. Impedance coupled to HREM should help to predict the clinical outcome and therefore personalize patient management. Nowadays, the management of esophageal dysmotility in patients with EA is essentially based on treatment of associated inflammation related to peptic or eosinophilic esophagitis.", "gemini": "Rối loạn vận động thực quản hầu như là phổ biến sau khi phục hồi teo thực quản (EA) và chủ yếu liên quan đến dị tật phát triển của thực quản. Rối loạn vận động thực quản liên quan đến bệnh sinh của nhiều triệu chứng và bệnh đi kèm liên quan đến EA như trào ngược dạ dày thực quản, các biến chứng về hô hấp và hít sặc, và các triệu chứng khó nuốt và rối loạn ăn uống. Đo áp lực thực quản độ phân giải cao (HREM) đã tạo điều kiện thuận lợi cho việc mô tả đặc điểm của rối loạn vận động, nhưng có sự tương quan không đầy đủ giữa các triệu chứng và các kiểu đo áp lực. Trở kháng kết hợp với HREM sẽ giúp dự đoán kết quả lâm sàng và do đó cá nhân hóa việc quản lý bệnh nhân. Ngày nay, việc quản lý rối loạn vận động thực quản ở những bệnh nhân bị EA về cơ bản dựa trên việc điều trị tình trạng viêm liên quan đến viêm thực quản do peptic hoặc bạch cầu ái toan."} +{"idx": 2000014, "en": "Even if European Union (EU) Member States are obliged to implement EU Directives 2000/54/EC on the protection of workers from risks related to exposure to biological agents at work, national biosafety regulations and practices varied from country to country. In fact, EU legislation on biological agents and genetically modified microorganisms is often not specific enough to ensure harmonization leading to difficulties in implementation for most laboratories. In the same way, biosecurity is a relatively new concept and a few EU Member States are known to have introduced national laboratory biosecurity legislation. In France, recent regulations have reinforced biosafety/biosecurity in containment level 3 (CL-3) laboratories but they concern a specific list of pathogens with no correlation in other European Members States. The objective of this review was to summarize European biosafety/biosecurity measures concerning CL-3 facilities focusing on French specificities. Essential requirements needed to preserve efficient biosafety measures when manipulating risk group 3 biological agents are highlighted. In addition, International, European and French standards related to containment laboratory planning, operation or biosafety equipment are described to clarify optimal biosafety and biosecurity requirements.", "gemini": "Ngay cả khi các quốc gia thành viên Liên minh Châu Âu (EU) có nghĩa vụ thực hiện Chỉ thị 2000/54/EC của EU về việc bảo vệ người lao động khỏi các rủi ro liên quan đến việc tiếp xúc với tác nhân sinh học tại nơi làm việc, các quy định và thực hành về an toàn sinh học quốc gia vẫn khác nhau giữa các quốc gia. Trên thực tế, luật của EU về tác nhân sinh học và vi sinh vật biến đổi gen thường không đủ cụ thể để đảm bảo sự hài hòa dẫn đến khó khăn trong việc triển khai đối với hầu hết các phòng thí nghiệm. Tương tự như vậy, an ninh sinh học là một khái niệm tương đối mới và một số quốc gia thành viên EU được biết là đã ban hành luật về an ninh sinh học phòng thí nghiệm quốc gia. Tại Pháp, các quy định gần đây đã tăng cường an toàn sinh học/an ninh sinh học ở các phòng thí nghiệm cấp độ kiểm soát 3 (CL-3) nhưng chúng chỉ liên quan đến một danh sách cụ thể các tác nhân gây bệnh mà không có mối tương quan nào ở các quốc gia thành viên Châu Âu khác. Mục tiêu của bài đánh giá này là tóm tắt các biện pháp an toàn sinh học/an ninh sinh học của Châu Âu liên quan đến các cơ sở CL-3 tập trung vào các đặc điểm cụ thể của Pháp. Các yêu cầu thiết yếu cần thiết để duy trì các biện pháp an toàn sinh học hiệu quả khi thao tác với các tác nhân sinh học nhóm rủi ro 3 được nêu bật. Ngoài ra, các tiêu chuẩn quốc tế, châu Âu và Pháp liên quan đến quy hoạch phòng thí nghiệm kiểm soát, vận hành hoặc thiết bị an toàn sinh học được mô tả để làm rõ các yêu cầu tối ưu về an toàn sinh học và an ninh sinh học."} +{"idx": 2000015, "en": "The interaction of volatile corrosion inhibitors (VCI), caprylate salt derivatives from amines, with zinc metallic surfaces is assessed by density functional theory (DFT) computer simulations, electrochemical impedance (EIS) measurements and humid chamber tests. The results obtained by the different methods were compared, and linear correlations were obtained between theoretical and experimental data. The correlations between experimental and theoretical results showed that the molecular size is the determining factor in the inhibition efficiency. The models used and experimental results indicated that dicyclohexylamine caprylate is the most efficient inhibitor.", "gemini": "Tương tác của chất ức chế ăn mòn dễ bay hơi (VCI), dẫn xuất muối caprylate từ amin, với bề mặt kim loại kẽm được đánh giá bằng mô phỏng máy tính lý thuyết hàm mật độ (DFT), phép đo trở kháng điện hóa (EIS) và thử nghiệm buồng ẩm. Các kết quả thu được bằng các phương pháp khác nhau đã được so sánh và có được mối tương quan tuyến tính giữa dữ liệu lý thuyết và thực nghiệm. Mối tương quan giữa kết quả thực nghiệm và lý thuyết cho thấy kích thước phân tử là yếu tố quyết định hiệu quả ức chế. Các mô hình được sử dụng và kết quả thực nghiệm chỉ ra rằng caprylate dicyclohexylamine là chất ức chế hiệu quả nhất."} +{"idx": 2000016, "en": "OBJECTIVE Traditional medical school curricula have not addressed fertility awareness-based methods (FABMs) of family planning. The objective of this study was to assess (1) 3-year medical students' knowledge of FABMs of family planning, (2) their confidence in utilizing that knowledge in patient care, and (3) to implement focused education on FABMs to improve knowledge and confidence. METHODS Third-year medical students at one institution in the United States were given a 10-question assessment at the beginning of their OB-GYN rotation. Two lectures about FABMs and their clinical applications were given during the rotation. Students were given the same questions at the end of the rotation. Each questionnaire consisted of eight questions to assess a student's knowledge of FABMs and two questions to assess the student's confidence in sharing and utilizing that information in a clinical setting. McNemar's test was used to analyze the data. RESULTS Two hundred seventy-seven students completed a pretest questionnaire and 196 students completed the posttest questionnaire. Medical knowledge improved from an initial test score of 38.99% to final test score of 53.57% (p < 0.05). Confidence in sharing FABM information with patients (0 = very uncomfortable; 5 = very comfortable) improved from 1.51 to 3.00 (p < 0.05). Confidence in utilizing FABM to diagnose and treat gynecologic/reproductive problems (0 = not very confident and 5 = very confident) improved from 1.01 to 3.15 (p < 0.05). CONCLUSION Medical schools may not include FABMs in OB-GYN curriculum; however, to patients, these methods remain a sought after and valid form of family planning. This study shows that brief, focused education can increase medical students' knowledge of and confidence with FABMs of family planning.", "gemini": "MỤC TIÊU Chương trình giảng dạy y khoa truyền thống chưa đề cập đến các phương pháp lập kế hoạch gia đình dựa trên nhận thức về khả năng sinh sản (FABM). Mục tiêu của nghiên cứu này là đánh giá (1) kiến thức của sinh viên y khoa năm thứ 3 về các phương pháp lập kế hoạch gia đình dựa trên FABM, (2) sự tự tin của họ khi sử dụng kiến thức đó trong việc chăm sóc bệnh nhân và (3) triển khai giáo dục tập trung về FABM để cải thiện kiến thức và sự tự tin. PHƯƠNG PHÁP Sinh viên y khoa năm thứ ba tại một cơ sở ở Hoa Kỳ đã được đánh giá 10 câu hỏi vào đầu đợt luân phiên OB-GYN của họ. Hai bài giảng về FABM và các ứng dụng lâm sàng của chúng đã được đưa ra trong quá trình luân phiên. Học sinh được đưa ra các câu hỏi tương tự vào cuối đợt luân phiên. Mỗi bảng câu hỏi bao gồm tám câu hỏi để đánh giá kiến thức của học sinh về FABM và hai câu hỏi để đánh giá sự tự tin của học sinh khi chia sẻ và sử dụng thông tin đó trong môi trường lâm sàng. Thử nghiệm McNemar được sử dụng để phân tích dữ liệu. KẾT QUẢ Hai trăm bảy mươi bảy sinh viên đã hoàn thành bảng câu hỏi tiền kiểm tra và 196 sinh viên đã hoàn thành bảng câu hỏi sau kiểm tra. Kiến thức y khoa được cải thiện từ điểm kiểm tra ban đầu là 38,99% lên điểm kiểm tra cuối cùng là 53,57% (p < 0,05). Sự tự tin khi chia sẻ thông tin FABM với bệnh nhân (0 = rất không thoải mái; 5 = rất thoải mái) đã được cải thiện từ 1,51 lên 3,00 (p < 0,05). Sự tự tin khi sử dụng FABM để chẩn đoán và điều trị các vấn đề phụ khoa / sinh sản (0 = không tự tin lắm và 5 = rất tự tin) đã được cải thiện từ 1,01 lên 3,15 (p < 0,05). KẾT LUẬN Các trường y có thể không đưa FABM vào chương trình giảng dạy OB-GYN; tuy nhiên, đối với bệnh nhân, các phương pháp này vẫn là một hình thức lập kế hoạch gia đình hợp lệ và được tìm kiếm. Nghiên cứu này cho thấy rằng giáo dục ngắn gọn, tập trung có thể nâng cao kiến thức và sự tự tin của sinh viên y khoa đối với các phương pháp lập kế hoạch gia đình dựa trên FABM."} +{"idx": 2000017, "en": "End-stage renal disease (ESRD) is characterized by the progressive deterioration of renal function that may compromise different tissues and organs. The major treatment indicated for patients with ESRD is kidney transplantation. However, the shortage of available organs, as well as the high rate of organ rejection, supports the need for new therapies. Thus, the implementation of tissue bioengineering to organ regeneration has emerged as an alternative to traditional organ transplantation. Decellularization of organs with chemical, physical, and/or biological agents generates natural scaffolds, which can serve as basis for tissue reconstruction. The recellularization of these scaffolds with different cell sources, such as stem cells or adult differentiated cells, can provide an organ with functionality and no immune response after in vivo transplantation on the host. Several studies have focused on improving these techniques, but until now, there is no optimal decellularization method for the kidney available yet. Herein, an overview of the current literature for kidney decellularization and whole-organ recellularization is presented, addressing the pros and cons of the actual techniques already developed, the methods adopted to evaluate the efficacy of the procedures, and the challenges to be overcome in order to achieve an optimal protocol.", "gemini": "Bệnh thận giai đoạn cuối (ESRD) được đặc trưng bởi sự suy giảm chức năng thận tiến triển có thể ảnh hưởng đến các mô và cơ quan khác nhau. Phương pháp điều trị chính được chỉ định cho bệnh nhân ESRD là ghép thận. Tuy nhiên, tình trạng thiếu các cơ quan có sẵn, cũng như tỷ lệ thải ghép cao, thúc đẩy nhu cầu về các phương pháp điều trị mới. Do đó, việc triển khai kỹ thuật mô để tái tạo cơ quan đã nổi lên như một phương pháp thay thế cho ghép tạng truyền thống. Quá trình khử tế bào của các cơ quan bằng các tác nhân hóa học, vật lý và / hoặc sinh học tạo ra các khung tự nhiên, có thể làm cơ sở cho việc tái tạo mô. Việc tái tạo tế bào của các khung này bằng các nguồn tế bào khác nhau, chẳng hạn như tế bào gốc hoặc tế bào biệt hóa ở người lớn, có thể cung cấp cho cơ quan chức năng và không có phản ứng miễn dịch sau khi cấy ghép vào vật chủ. Một số nghiên cứu đã tập trung vào việc cải thiện các kỹ thuật này, nhưng cho đến nay, vẫn chưa có phương pháp khử tế bào tối ưu cho thận. Tại đây, chúng tôi trình bày tổng quan về các tài liệu hiện có về khử tế bào thận và tái tạo toàn bộ cơ quan, đề cập đến ưu và nhược điểm của các kỹ thuật thực tế đã được phát triển, các phương pháp được áp dụng để đánh giá hiệu quả của các thủ thuật và những thách thức cần phải vượt qua để đạt được một giao thức tối ưu."} +{"idx": 2000018, "en": "Eosinophils are differentiated granulocytes that are recruited from the bone marrow to sites of inflammation via the vascular system. Allergic asthma is characterized by the presence of large numbers of eosinophils in the lungs and airways. Due to their capacity to rapidly release inflammatory mediators such as cytokines, chemokines, growth factors, and cytotoxic granule proteins upon stimulation, eosinophils play a critical role in pro-inflammatory processes in allergen-exposed lungs. Identifying key players and understanding the molecular mechanisms directing eosinophil trafficking and recruitment to inflamed airways is a key to developing therapeutic strategies to limit their influx. Recent studies have brought to light the important role of glycans and glycan binding proteins in regulating recruitment of eosinophils. In addition to the role of previously identified eosinophil- and endothelial-expressed adhesion molecules in mediating eosinophil trafficking and recruitment to the inflamed airways, studies have also indicated a role for galectins (galectin-3) in this process. Galectins are mammalian lectins expressed by various cell types including eosinophils. Intracellularly, they can regulate biological processes such as cell motility. Extracellularly, galectins interact with β-galactosides in cell surface-expressed glycans to regulate cellular responses like production of inflammatory mediators, cell adhesion, migration, and apoptosis. Eosinophils express galectins intracellularly or on the cell surface where they interact with cell surface glycoconjugate receptors. Depending on the type (galectin-1, -3, etc.) and location (extracellular or intracellular, endogenous or exogenously delivered), galectins differentially regulate eosinophil recruitment, activation, and apoptosis and thus exert a pro- or anti-inflammatory outcome. Here, we have reviewed information pertaining to galectins (galectin-1, -3 -9, and -10) that are expressed by eosinophils themselves and/or other cells that play a role in eosinophil recruitment and function in the context of allergic asthma and their potential use as disease biomarkers or therapeutic targets for immunomodulation.", "gemini": "Eosinophil là các hạt bạch cầu biệt hóa được tuyển dụng từ tủy xương đến các vị trí viêm thông qua hệ thống mạch máu. Hen dị ứng được đặc trưng bởi sự hiện diện của một số lượng lớn eosinophil trong phổi và đường hô hấp. Do khả năng giải phóng nhanh chóng các chất trung gian gây viêm như cytokine, chemokine, yếu tố tăng trưởng và protein hạt tế bào gây độc khi được kích thích, eosinophil đóng vai trò quan trọng trong các quá trình tiền viêm ở phổi tiếp xúc với chất gây dị ứng. Xác định những tác nhân chính và hiểu được các cơ chế phân tử chỉ đạo sự di chuyển và tuyển dụng eosinophil đến đường hô hấp bị viêm là chìa khóa để phát triển các chiến lược điều trị nhằm hạn chế sự xâm nhập của chúng. Các nghiên cứu gần đây đã làm sáng tỏ vai trò quan trọng của glycan và protein liên kết glycan trong việc điều hòa sự tuyển dụng eosinophil. Ngoài vai trò của các phân tử bám dính được biểu hiện trên tế bào nội mô và eosinophil đã được xác định trước đó trong việc trung gian sự di chuyển và tuyển dụng eosinophil đến đường hô hấp bị viêm, các nghiên cứu cũng chỉ ra vai trò của galectin (galectin-3) trong quá trình này. Galectin là lectin ở động vật có vú được biểu hiện bởi nhiều loại tế bào khác nhau bao gồm cả eosinophil. Trong tế bào, chúng có thể điều hòa các quá trình sinh học như vận động tế bào. Ngoài tế bào, galectin tương tác với β-galactosid trong glycan được biểu hiện trên bề mặt tế bào để điều hòa các phản ứng của tế bào như sản xuất chất trung gian gây viêm, bám dính tế bào, di chuyển và apoptosis. Eosinophil biểu hiện galectin trong tế bào hoặc trên bề mặt tế bào, nơi chúng tương tác với các thụ thể glycoconjugate trên bề mặt tế bào. Tùy thuộc vào loại (galectin-1, -3, v.v.) và vị trí (ngoài tế bào hoặc trong tế bào, nội sinh hoặc ngoại sinh), galectin điều hòa sự tuyển dụng, hoạt hóa và apoptosis của eosinophil khác nhau và do đó tạo ra kết quả chống hoặc chống viêm. Tại đây, chúng tôi đã xem xét thông tin liên quan đến galectin (galectin-1, -3 -9 và -10) được biểu hiện bởi chính eosinophil và/hoặc các tế bào khác đóng vai trò trong việc tuyển dụng và chức năng eosinophil trong bối cảnh hen dị ứng và tiềm năng sử dụng chúng làm sinh học đánh dấu bệnh hoặc mục tiêu điều trị để điều hòa miễn dịch."} +{"idx": 2000019, "en": "Congenital heart defects (CHDs) affect approximately 1% of live births and are a major source of childhood morbidity and mortality even in countries with advanced healthcare systems. Along with phenotypic heterogeneity, the underlying etiology of CHDs is multifactorial, involving genetic, epigenetic, and/or environmental contributors. Clear dissection of the underlying mechanism is a powerful step to establish individualized therapies. However, the majority of CHDs are yet to be clearly diagnosed for the underlying genetic and environmental factors, and even less with effective therapies. Although the survival rate for CHDs is steadily improving, there is still a significant unmet need for refining diagnostic precision and establishing targeted therapies to optimize life quality and to minimize future complications. In particular, proper identification of disease associated genetic variants in humans has been challenging, and this greatly impedes our ability to delineate gene-environment interactions that contribute to the pathogenesis of CHDs. Implementing a systematic multileveled approach can establish a continuum from phenotypic characterization in the clinic to molecular dissection using combined next-generation sequencing platforms and validation studies in suitable models at the bench. Key elements necessary to advance the field are: first, proper delineation of the phenotypic spectrum of CHDs; second, defining the molecular genotype/phenotype by combining whole-exome sequencing and transcriptome analysis; third, integration of phenotypic, genotypic, and molecular datasets to identify molecular network contributing to CHDs; fourth, generation of relevant disease models and multileveled experimental investigations. In order to achieve all these goals, access to high-quality biological specimens from well-defined patient cohorts is a crucial step. Therefore, establishing a CHD BioCore is an essential infrastructure and a critical step on the path toward precision child health cardiovascular medicine.", "gemini": "Các khuyết tật tim bẩm sinh (CHD) ảnh hưởng đến khoảng 1% trẻ sinh ra sống và là nguyên nhân chính gây tử vong và bệnh tật ở trẻ em ngay cả ở những quốc gia có hệ thống chăm sóc sức khỏe tiên tiến. Cùng với tính không đồng nhất về kiểu hình, nguyên nhân cơ bản của CHD là đa yếu tố, liên quan đến các yếu tố di truyền, biểu sinh và/hoặc môi trường. Phân tích rõ ràng cơ chế cơ bản là một bước mạnh mẽ để thiết lập các liệu pháp cá nhân hóa. Tuy nhiên, phần lớn các CHD vẫn chưa được chẩn đoán rõ ràng về các yếu tố di truyền và môi trường cơ bản, và thậm chí ít hơn với các liệu pháp hiệu quả. Mặc dù tỷ lệ sống sót của CHD đang được cải thiện ổn định, nhưng vẫn còn nhu cầu chưa được đáp ứng đáng kể về việc tinh chỉnh độ chính xác chẩn đoán và thiết lập các liệu pháp nhắm mục tiêu để tối ưu hóa chất lượng cuộc sống và giảm thiểu các biến chứng trong tương lai. Đặc biệt, việc xác định đúng các biến thể di truyền liên quan đến bệnh ở người là một thách thức lớn và điều này cản trở rất nhiều khả năng mô tả các tương tác gen-môi trường góp phần vào quá trình sinh bệnh của CHD. Việc triển khai một phương pháp tiếp cận đa cấp có hệ thống có thể thiết lập một sự liên tục từ đặc điểm kiểu hình trong lâm sàng đến phân tích phân tử bằng cách sử dụng kết hợp các nền tảng giải trình tự thế hệ tiếp theo và các nghiên cứu xác thực trong các mô hình phù hợp tại phòng thí nghiệm. Các yếu tố chính cần thiết để phát triển lĩnh vực này là: thứ nhất, mô tả đúng phổ kiểu hình của CHD; thứ hai, xác định kiểu gen/kiểu hình phân tử bằng cách kết hợp giải trình tự toàn bộ exome và phân tích bản sao; thứ ba, tích hợp dữ liệu kiểu hình, kiểu gen và phân tử để xác định mạng lưới phân tử góp phần vào CHD; thứ tư, tạo ra các mô hình bệnh liên quan và các cuộc điều tra thực nghiệm đa cấp. Để đạt được tất cả các mục tiêu này, việc tiếp cận các mẫu vật sinh học chất lượng cao từ các nhóm bệnh nhân được xác định rõ là một bước rất quan trọng. Do đó, việc thành lập CHD BioCore là một cơ sở hạ tầng thiết yếu và là một bước quan trọng trên con đường hướng tới y học tim mạch nhi khoa chính xác."} +{"idx": 2000020, "en": "Diabetic cardiomyopathy (DCM) or diabetes-induced cardiac dysfunction is a direct consequence of uncontrolled metabolic syndrome and is widespread in US population and worldwide. Despite of the heterogeneous and distinct features of DCM, the clinical relevance of DCM is now becoming established. DCM progresses to pathological cardiac remodeling with the higher risk of heart attack and subsequent heart failure in diabetic patients. In this review, we emphasize lipid substrate quality and the phenotypic, metabolic, and biochemical stressors of DCM in the rodent and human pathophysiology. We discuss lipoxygenase signaling in the inflammatory pathway with multiple contributing and confounding factors leading to DCM. Additionally, emerging biochemical pathways are emphasized to make progress toward therapeutic advancement to treat DCM.", "gemini": "Bệnh cơ tim do đái tháo đường (DCM) hay rối loạn chức năng tim do đái tháo đường là hậu quả trực tiếp của hội chứng chuyển hóa không kiểm soát và phổ biến ở dân số Hoa Kỳ và trên toàn thế giới. Bất chấp các đặc điểm không đồng nhất và khác biệt của DCM, nhưng hiện nay liên quan lâm sàng của DCM đã được thiết lập. DCM tiến triển thành tình trạng tái cấu trúc tim bệnh lý với nguy cơ cao bị đau tim và suy tim sau đó ở bệnh nhân tiểu đường. Trong bài đánh giá này, chúng tôi nhấn mạnh đến chất lượng cơ chất lipid và các yếu tố gây căng thẳng về kiểu hình, chuyển hóa và sinh hóa của DCM trong sinh lý bệnh ở động vật gặm nhấm và ở người. Chúng tôi thảo luận về tín hiệu lipoxygenase trong con đường viêm với nhiều yếu tố góp phần và gây nhiễu dẫn đến DCM. Ngoài ra, các con đường sinh hóa mới nổi được nhấn mạnh để tiến tới tiến bộ trong điều trị để điều trị DCM."} +{"idx": 2000021, "en": "Antimicrobial resistance in livestock is a matter of general concern. To develop hygiene measures and methods for resistance prevention and control, epidemiological studies on a population level are needed to detect factors associated with antimicrobial resistance in livestock holdings. In general, regression models are used to describe these relationships between environmental factors and resistance outcome. Besides the study design, the correlation structures of the different outcomes of antibiotic resistance and structural zero measurements on the resistance outcome as well as on the exposure side are challenges for the epidemiological model building process. The use of appropriate regression models that acknowledge these complexities is essential to assure valid epidemiological interpretations. The aims of this paper are (i) to explain the model building process comparing several competing models for count data (negative binomial model, quasi-Poisson model, zero-inflated model, and hurdle model) and (ii) to compare these models using data from a cross-sectional study on antibiotic resistance in animal husbandry. These goals are essential to evaluate which model is most suitable to identify potential prevention measures. The dataset used as an example in our analyses was generated initially to study the prevalence and associated factors for the appearance of cefotaxime-resistant Escherichia coli in 48 German fattening pig farms. For each farm, the outcome was the count of samples with resistant bacteria. There was almost no overdispersion and only moderate evidence of excess zeros in the data. Our analyses show that it is essential to evaluate regression models in studies analyzing the relationship between environmental factors and antibiotic resistances in livestock. After model comparison based on evaluation of model predictions, Akaike information criterion, and Pearson residuals, here the hurdle model was judged to be the most appropriate model.", "gemini": "Kháng thuốc ở gia súc là vấn đề đáng quan tâm chung. Để phát triển các biện pháp vệ sinh và phương pháp phòng ngừa và kiểm soát tình trạng kháng thuốc, cần tiến hành các nghiên cứu dịch tễ học ở cấp quần thể để phát hiện các yếu tố liên quan đến tình trạng kháng thuốc ở các trang trại chăn nuôi gia súc. Nói chung, các mô hình hồi quy được sử dụng để mô tả mối quan hệ giữa các yếu tố môi trường và kết quả kháng thuốc. Bên cạnh thiết kế nghiên cứu, các cấu trúc tương quan của các kết quả khác nhau về tình trạng kháng thuốc và các phép đo bằng không về cấu trúc đối với kết quả kháng thuốc cũng như về phía phơi nhiễm là những thách thức đối với quá trình xây dựng mô hình dịch tễ học. Việc sử dụng các mô hình hồi quy phù hợp để thừa nhận những phức tạp này là rất cần thiết để đảm bảo các diễn giải dịch tễ học có giá trị. Mục tiêu của bài báo này là (i) giải thích quá trình xây dựng mô hình bằng cách so sánh một số mô hình cạnh tranh cho dữ liệu đếm (mô hình nhị thức âm, mô hình quasi-Poisson, mô hình tăng cường bằng không và mô hình rào cản) và (ii) so sánh các mô hình này bằng cách sử dụng dữ liệu từ một nghiên cứu cắt ngang về tình trạng kháng thuốc ở ngành chăn nuôi. Những mục tiêu này là rất cần thiết để đánh giá mô hình nào phù hợp nhất để xác định các biện pháp phòng ngừa tiềm năng. Bộ dữ liệu được sử dụng làm ví dụ trong các phân tích của chúng tôi ban đầu được tạo ra để nghiên cứu tỷ lệ mắc và các yếu tố liên quan đến sự xuất hiện của vi khuẩn Escherichia coli kháng cefotaxime ở 48 trang trại nuôi lợn vỗ béo của Đức. Đối với mỗi trang trại, kết quả là số lượng mẫu có vi khuẩn kháng thuốc. Gần như không có sự phân tán quá mức và chỉ có bằng chứng vừa phải về số không thừa trong dữ liệu. Các phân tích của chúng tôi cho thấy rằng việc đánh giá các mô hình hồi quy trong các nghiên cứu phân tích mối quan hệ giữa các yếu tố môi trường và tình trạng kháng thuốc ở gia súc là rất cần thiết. Sau khi so sánh mô hình dựa trên đánh giá dự đoán của mô hình, tiêu chuẩn thông tin Akaike và các giá trị dư Pearson, mô hình rào cản được đánh giá là mô hình phù hợp nhất."} +{"idx": 2000022, "en": "Flame retardants are a wide group of chemicals used by the industry to avoid combustion of materials. These substances are commonly found in plastics, electronic equipment, fabrics, and in many other everyday articles. Subsequently, ubiquitous environmental contamination by these common chemical is frequently reported. In the present study, we have evaluated the level of exposure to polychlorinated biphenyls (PCBs), brominated diphenyl ethers (BDEs), and organophosphorous flame retardants (OPFRs) in pet cats through the analysis of their serum. We also analyzed the level exposure to such chemicals in a series of 20 cat owners, trying to disclose the role of pet cats as sentinel species of human exposure to FRs. Our results showed that PCBs, banned 40 years ago, showed the lowest levels of exposure, followed by BDEs-banned recently. Congeners PCB-138 and PCB-180 were detected in ≥50% of the series, while BDE-47 was detected in near 90% of the pet cats. On the other hand, the highest levels were that of OPFRs, whose pattern of detection was similar to that observed in humans, thus suggesting a potential role of cats as a sentinel species for human exposure to these currently used FRs. Six out of 11 OPFRs determined [2-ethylhexyldiphenyl phosphate, tributylphosphate, triisobutylphosphate, triphenylphosphate, tris (2-chloroethyl) phosphate, and tris (2-chloroisopropyl) phosphate] were detected in 100% of the samples. It will be interesting to perform future studied aimed to elucidating the potential toxicological effects of these highly detected chemicals both, in cats and humans.", "gemini": "Chất chống cháy là một nhóm hóa chất rộng được ngành công nghiệp sử dụng để tránh vật liệu bị cháy. Những chất này thường được tìm thấy trong nhựa, thiết bị điện tử, vải và nhiều vật dụng hàng ngày khác. Do đó, tình trạng ô nhiễm môi trường phổ biến bởi hóa chất thông thường này thường xuyên được báo cáo. Trong nghiên cứu hiện tại, chúng tôi đã đánh giá mức độ phơi nhiễm với polychlorinated biphenyls (PCB), brominated diphenyl ethers (BDE) và chất chống cháy organophosphorous (OPFR) ở mèo cưng thông qua việc phân tích huyết thanh của chúng. Chúng tôi cũng đã phân tích mức độ phơi nhiễm với các hóa chất như vậy ở một loạt 20 chủ sở hữu mèo, cố gắng tiết lộ vai trò của mèo cưng như loài cảnh báo về việc con người tiếp xúc với FR. Kết quả của chúng tôi cho thấy PCB, bị cấm cách đây 40 năm, cho thấy mức độ phơi nhiễm thấp nhất, tiếp theo là BDE bị cấm gần đây. Các đồng loại PCB-138 và PCB-180 được phát hiện ở ≥50% của loạt, trong khi BDE-47 được phát hiện ở gần 90% mèo cưng. Mặt khác, mức độ cao nhất là OPFR, có kiểu phát hiện tương tự như kiểu quan sát thấy ở người, do đó gợi ý vai trò tiềm năng của mèo như một loài cảnh báo về việc con người tiếp xúc với các FR hiện đang sử dụng này. Sáu trong số 11 OPFR được xác định [2-ethylhexyldiphenyl phosphate, tributylphosphate, triisobutylphosphate, triphenylphosphate, tris (2-chloroethyl) phosphate và tris (2-chloroisopropyl) phosphate] được phát hiện ở 100% mẫu. Sẽ rất thú vị khi thực hiện các nghiên cứu trong tương lai nhằm làm sáng tỏ các tác động độc hại tiềm ẩn của những hóa chất được phát hiện nhiều này ở cả mèo và người."} +{"idx": 2000023, "en": "Vpr is an HIV-1 accessory protein that plays numerous roles during viral replication, and some of which are cell type dependent. To test the hypothesis that HIV-1 tropism extends beyond the envelope into the vpr gene, studies were performed to identify the associations between coreceptor usage and Vpr variation in HIV-1-infected patients. Colinear HIV-1 Env-V3 and Vpr amino acid sequences were obtained from the LANL HIV-1 sequence database and from well-suppressed patients in the Drexel/Temple Medicine CNS AIDS Research and Eradication Study (CARES) Cohort. Genotypic classification of Env-V3 sequences as X4 (CXCR4-utilizing) or R5 (CCR5-utilizing) was used to group colinear Vpr sequences. To reveal the sequences associated with a specific coreceptor usage genotype, Vpr amino acid sequences were assessed for amino acid diversity and Jensen-Shannon divergence between the two groups. Five amino acid alphabets were used to comprehensively examine the impact of amino acid substitutions involving side chains with similar physiochemical properties. Positions 36, 37, 41, 89, and 96 of Vpr were characterized by statistically significant divergence across multiple alphabets when X4 and R5 sequence groups were compared. In addition, consensus amino acid switches were found at positions 37 and 41 in comparisons of the R5 and X4 sequence populations. These results suggest an evolutionary link between Vpr and gp120 in HIV-1-infected patients.", "gemini": "Vpr là một protein phụ của HIV-1 đóng nhiều vai trò trong quá trình sao chép vi-rút và một số vai trò phụ thuộc vào loại tế bào. Để kiểm tra giả thuyết rằng tính hướng đích của HIV-1 mở rộng ra ngoài lớp vỏ vào gen vpr, các nghiên cứu đã được thực hiện để xác định mối liên quan giữa việc sử dụng đồng thụ thể và biến thể Vpr ở những bệnh nhân nhiễm HIV-1. Trình tự axit amin Env-V3 và Vpr của HIV-1 được lấy từ cơ sở dữ liệu trình tự HIV-1 của LANL và từ những bệnh nhân được ức chế tốt trong Nghiên cứu và xóa bỏ AIDS ở hệ thần kinh trung ương của Drexel/Temple Medicine (CARES). Phân loại kiểu gen của trình tự Env-V3 là X4 (sử dụng CXCR4) hoặc R5 (sử dụng CCR5) được sử dụng để nhóm các trình tự Vpr cùng tuyến. Để phát hiện các trình tự liên quan đến kiểu gen sử dụng đồng thụ thể cụ thể, các trình tự axit amin Vpr được đánh giá về tính đa dạng của axit amin và độ phân kỳ Jensen-Shannon giữa hai nhóm. Năm bảng chữ cái axit amin đã được sử dụng để kiểm tra toàn diện tác động của sự thay thế axit amin liên quan đến các chuỗi bên có tính chất lý hóa học tương tự. Các vị trí 36, 37, 41, 89 và 96 của Vpr được đặc trưng bởi sự phân kỳ có ý nghĩa thống kê trên nhiều bảng chữ cái khi so sánh các nhóm trình tự X4 và R5. Ngoài ra, sự chuyển đổi axit amin đồng thuận được tìm thấy ở các vị trí 37 và 41 trong các so sánh giữa các quần thể trình tự R5 và X4. Những kết quả này gợi ý một mối liên hệ tiến hóa giữa Vpr và gp120 ở những bệnh nhân nhiễm HIV-1."} +{"idx": 2000024, "en": "117 postmenopausal women were divided into Normal, Bone loss (BL), and Osteoporosis group. Compared with Normal group (120.96 ± 43.18 μg/L), the serum ferritin (Fer) in BL (223.37 ± 130.27 μg/L) and Osteoporosis group (307.50 ± 161.48 μg/L) was significantly increased (p < 0.05). Fer level was negatively correlated with BMD (p < 0.01). TRACP levels in Osteoporosis group (4.37 ± 1.69 U/L) were significantly higher than Normal group (4.10 ± 1.60 U/L, p < 0.05). ALP levels in Osteoporosis group (112.06 ± 62.05 U/L) were significantly upregulated compared with Normal group (80.22 ± 14.94 U/L, p < 0.05). β-CTX and PINP were the degradation products of type I collagen. β-CTX levels in Osteoporosis group (667.90 ± 316.55 ng/L) were significantly increased compared with Normal group (406.06 ± 112.12 ng/L, p < 0.05). PINP levels in Osteoporosis group (78.03 ± 37.31 μg/L) were significantly higher than Normal group (37.60 ± 13.17 μg/L, p < 0.01). More importantly, there was a positive correlation between serum Fer and PINP (p < 0.01). Serum Fer showed a positive correlation of serum β-CTX (p < 0.01). The overloaded iron improved the degradation of type I collagen.", "gemini": "117 phụ nữ sau mãn kinh được chia thành nhóm Bình thường, Mất xương (BL) và Loãng xương. So với nhóm Bình thường (120,96 ± 43,18 μg/L), thì ferritin huyết thanh (Fer) ở nhóm BL (223,37 ± 130,27 μg/L) và nhóm Loãng xương (307,50 ± 161,48 μg/L) tăng đáng kể (p < 0,05). Nồng độ Fer có tương quan nghịch với BMD (p < 0,01). Nồng độ TRACP ở nhóm Loãng xương (4,37 ± 1,69 U/L) cao hơn đáng kể so với nhóm Bình thường (4,10 ± 1,60 U/L, p < 0,05). Nồng độ ALP ở nhóm Loãng xương (112,06 ± 62,05 U/L) tăng đáng kể so với nhóm Bình thường (80,22 ± 14,94 U/L, p < 0,05). β-CTX và PINP là các sản phẩm thoái hóa của collagen loại I. Nồng độ β-CTX ở nhóm Loãng xương (667,90 ± 316,55 ng/L) tăng đáng kể so với nhóm Bình thường (406,06 ± 112,12 ng/L, p < 0,05). Nồng độ PINP ở nhóm Loãng xương (78,03 ± 37,31 μg/L) cao hơn đáng kể so với nhóm Bình thường (37,60 ± 13,17 μg/L, p < 0,01). Quan trọng hơn, có tương quan thuận giữa Fer huyết thanh và PINP (p < 0,01). Fer huyết thanh có tương quan thuận với β-CTX huyết thanh (p < 0,01). Sắt quá tải làm cải thiện quá trình thoái hóa collagen loại I."} +{"idx": 2000025, "en": "Root canal sealers with antimicrobial activity are highly beneficial; therefore, their antimicrobial properties could be improved by incorporation of antimicrobial agents. In the present study, the release of the quaternary ammonium compounds from endodontic sealers admixed with either benzalkonium chloride (BC) or cetylpyridinium chloride (CPC) at loadings of 2% wt was monitored. The effect of these additives on the compressive strengths and their release from the sealers was determined after 1 and 4 weeks. All of the materials studied were found to be capable of releasing antimicrobial additive in useful quantities. The release of CPC occurred to a statistically significant greater extent than BC for all materials. The addition of both BC and CPC generally decreased the compressive strength of all the endodontic sealers, with the exception of CPC in AH Plus, where the compressive strength was significantly increased. This suggests that, for these endodontic sealers, the antimicrobial additives alter the setting chemistry. AH Plus is an epoxy-based material cured with an amine, and in this case the increase in compressive strength with CPC is attributed to an enhanced cure reaction with this system. In all other cases, the additive inhibited the cure reaction to a greater or lesser extent.", "gemini": "Chất trám ống tủy có hoạt tính kháng khuẩn rất có lợi; do đó, có thể cải thiện các đặc tính kháng khuẩn của chúng bằng cách kết hợp các tác nhân kháng khuẩn. Trong nghiên cứu hiện tại, việc giải phóng các hợp chất amoni bậc bốn từ chất trám nha khoa được trộn với benzalkonium chloride (BC) hoặc cetylpyridinium chloride (CPC) với nồng độ 2% trọng lượng đã được theo dõi. Tác động của các chất phụ gia này đến độ bền nén và sự giải phóng của chúng khỏi chất trám đã được xác định sau 1 và 4 tuần. Tất cả các vật liệu được nghiên cứu đều có khả năng giải phóng chất phụ gia kháng khuẩn với số lượng hữu ích. Sự giải phóng CPC xảy ra ở mức độ lớn hơn đáng kể về mặt thống kê so với BC đối với tất cả các vật liệu. Việc bổ sung cả BC và CPC thường làm giảm độ bền nén của tất cả các chất trám nha khoa, ngoại trừ CPC trong AH Plus, trong đó độ bền nén tăng đáng kể. Điều này cho thấy rằng, đối với các chất trám nha khoa này, các chất phụ gia kháng khuẩn làm thay đổi quá trình đông kết. AH Plus là vật liệu gốc epoxy được đông kết bằng amin, và trong trường hợp này, sự gia tăng độ bền nén với CPC được cho là do phản ứng đông kết tăng cường với hệ thống này. Trong mọi trường hợp khác, chất phụ gia ức chế phản ứng đông kết ở mức độ lớn hơn hoặc nhỏ hơn."} +{"idx": 2000026, "en": "OBJECTIVES To assess the impact of combining low-tube voltage acquisition with iterative reconstruction (IR) techniques on the iodine dose in coronary CTA. METHODS Three minipigs underwent CCTA to compare a standard of care protocol with two alternative study protocols combining low-tube voltage and low iodine dose with IR. Image quality was evaluated objectively by the CT value, signal-to-noise ratio (SNR), and contrast-to-noise ratio (CNR) in the main coronary arteries and aorta and subjectively by expert reading. Statistics were performed by Mann-Whitney U test and Chi-square analysis. RESULTS Despite reduced iodine dose, both study protocols maintained CT values, SNR, and CNR compared to the standard of care protocol. Expert readings confirmed these findings; all scans were perceived to be of at least diagnostically acceptable quality on all evaluated parameters allowing image interpretation. No statistical differences were observed (all p values > 0.11), except for streak artifacts (p = 0.02) which were considered to be more severe, although acceptable, with the 80 kVp protocol. CONCLUSIONS Reduced tube voltage in combination with IR allows a total iodine dose reduction between 37 and 50%, by using contrast media with low iodine concentrations of 200 and 160 mg I/mL, while maintaining image quality.", "gemini": "MỤC TIÊU Đánh giá tác động của việc kết hợp thu nhận điện áp ống thấp với các kỹ thuật tái tạo lặp (IR) đối với liều iốt trong CTA động mạch vành. PHƯƠNG PHÁP Ba con lợn nhỏ đã trải qua CCTA để so sánh một giao thức chăm sóc tiêu chuẩn với hai giao thức nghiên cứu thay thế kết hợp điện áp ống thấp và liều iốt thấp với IR. Chất lượng hình ảnh được đánh giá khách quan bằng giá trị CT, tỷ lệ tín hiệu trên nhiễu (SNR) và tỷ lệ tương phản trên nhiễu (CNR) ở các động mạch vành chính và động mạch chủ và đánh giá chủ quan bằng cách đọc của chuyên gia. Thống kê được thực hiện bằng kiểm tra Mann-Whitney U và phân tích Chi-square. KẾT QUẢ Mặc dù liều iốt giảm, cả hai giao thức nghiên cứu đều duy trì các giá trị CT, SNR và CNR so với giao thức chăm sóc tiêu chuẩn. Các kết quả đọc của chuyên gia đã xác nhận những phát hiện này; tất cả các ảnh chụp đều được coi là có chất lượng ít nhất là có thể chấp nhận được về mặt chẩn đoán trên tất cả các thông số được đánh giá cho phép giải thích hình ảnh. Không có sự khác biệt thống kê nào được quan sát (tất cả các giá trị p > 0,11), ngoại trừ các hiện vật vệt (p = 0,02) được coi là nghiêm trọng hơn, mặc dù có thể chấp nhận được, với giao thức 80 kVp. KẾT LUẬN Giảm điện áp ống kết hợp với IR cho phép giảm tổng liều iốt từ 37 đến 50%, bằng cách sử dụng thuốc cản quang có nồng độ iốt thấp là 200 và 160 mg I/mL, trong khi vẫn duy trì chất lượng hình ảnh."} +{"idx": 2000027, "en": "Jatropha curcas cake, a by-product of biodiesel production, is rich in protein and has potential to be used in livestock feed; however, the presence of antinutritional factors and phorbol esters limits its use. Thus, this study investigated toxicological and reproductive effects in male Wistar rats after subchronic exposure to J. curcas cake subjected to detoxification procedures. Rats were divided into seven groups (n = 10) and treated for 60 days. The control group received commercial feed, while experimental groups received a diet containing 5% J. curcas cake nonhydrolyzed or hydrolyzed with 5 M NaOH. The cakes were unwashed or washed with ethanol or water and were autoclaved at 121°C for 30 minutes. Alkaline hydrolysis combined with ethanol washing decreased the phorbol ester concentration in the cake by 98%. Histopathological findings included diffuse degeneration of the liver and edema around the pulmonary vessels in the nonhydrolyzed groups. In addition, nontreated females mated with males of nonhydrolyzed unwashed group showed a decreased number of live fetuses and an increased placental weight. There were no signs of toxicity in rats given hydrolyzed cakes washed and unwashed, indicating that alkaline hydrolysis associated with heat treatment is an efficient method for detoxification of the J. curcas cake.", "gemini": "Bánh dầu Jatropha curcas, một sản phẩm phụ của quá trình sản xuất biodiesel, giàu protein và có tiềm năng sử dụng làm thức ăn chăn nuôi; tuy nhiên, sự hiện diện của các yếu tố kháng dinh dưỡng và este phorbol hạn chế việc sử dụng nó. Do đó, nghiên cứu này đã điều tra các tác động về độc tính và sinh sản ở chuột Wistar đực sau khi tiếp xúc bán mãn tính với bánh dầu J. curcas đã qua các quy trình giải độc. Chuột được chia thành bảy nhóm (n = 10) và được xử lý trong 60 ngày. Nhóm đối chứng được cho ăn thức ăn thương mại, trong khi các nhóm thực nghiệm được cho ăn chế độ ăn có chứa 5% bánh dầu J. curcas chưa thủy phân hoặc đã thủy phân bằng NaOH 5M. Các loại bánh dầu này không được rửa hoặc được rửa bằng etanol hoặc nước và được hấp tiệt ở 121°C trong 30 phút. Thủy phân kiềm kết hợp với rửa bằng etanol làm giảm nồng độ este phorbol trong bánh dầu xuống 98%. Các phát hiện mô bệnh học bao gồm thoái hóa lan tỏa ở gan và phù quanh các mạch máu phổi ở các nhóm không thủy phân. Ngoài ra, những con cái chưa được xử lý giao phối với những con đực thuộc nhóm không thủy phân chưa rửa cho thấy số lượng thai nhi sống giảm và trọng lượng nhau thai tăng. Không có dấu hiệu ngộ độc ở những con chuột được cho ăn bánh dầu đã thủy phân đã rửa và chưa rửa, cho thấy phương pháp thủy phân kiềm kết hợp với xử lý nhiệt là một phương pháp hiệu quả để giải độc bánh dầu J. curcas."} +{"idx": 2000028, "en": "This study reports the activity induced by (1E,4E)-2-methyl-1,5-bis(4-nitrophenyl)penta-1,4-dien-3-one (A3K2A3) against Trypanosoma cruzi. This compound showed trypanocidal activity against the multiplicative epimastigote and amastigote forms of this protozoan, with IC50 values of 1.99 ± 0.17 and 1.20 ± 0.16 μM, respectively, and EC50 value of 15.57 ± 0.34 μM against trypomastigotes. The combination of A3K2A3 with benznidazole or ketoconazole demonstrated strong synergism, increasing effectiveness against trypomastigotes or epimastigotes of T. cruzi. In addition, the drug combination of A3K2A3 with benznidazole or ketoconazole on LLCMK2 cells demonstrated an antagonist effect, which resulted in greater protection of the cells from drug damage. The combination of the compound with fluconazole was not effective. Transmission and scanning electron micrographs showed changes on parasites, mainly in the cytoplasmatic membrane, nucleus, mitochondrion, and Golgi complex, and a large increase in the number of autophagosome-like structures and lipid-storage bodies, accompanied by volume reduction and rounding of the parasite. A3K2A3 might be a promising compound against T. cruzi.", "gemini": "Nghiên cứu này báo cáo hoạt động được gây ra bởi (1E,4E)-2-methyl-1,5-bis(4-nitrophenyl)penta-1,4-dien-3-one (A3K2A3) chống lại Trypanosoma cruzi. Hợp chất này cho thấy hoạt tính diệt trypanocidal đối với các dạng epimastigote và amastigote nhân lên của loại nguyên sinh động vật này, với giá trị IC50 lần lượt là 1,99 ± 0,17 và 1,20 ± 0,16 μM và giá trị EC50 là 15,57 ± 0,34 μM đối với trypomastigotes. Sự kết hợp của A3K2A3 với benznidazole hoặc ketoconazole cho thấy sự hiệp đồng mạnh mẽ, tăng hiệu quả chống lại trypomastigotes hoặc epimastigotes của T. cruzi. Ngoài ra, sự kết hợp thuốc của A3K2A3 với benznidazole hoặc ketoconazole trên các tế bào LLCMK2 cho thấy tác dụng đối kháng, dẫn đến khả năng bảo vệ tế bào khỏi tác hại của thuốc tốt hơn. Sự kết hợp của hợp chất với fluconazole không có hiệu quả. Ảnh chụp kính hiển vi điện tử quét và truyền qua cho thấy những thay đổi trên ký sinh trùng, chủ yếu ở màng tế bào chất, nhân, ty thể và phức hợp Golgi, và số lượng lớn các cấu trúc giống như autophagosome và các thể dự trữ lipid tăng lên, kèm theo giảm thể tích và làm tròn ký sinh trùng. A3K2A3 có thể là một hợp chất đầy hứa hẹn chống lại T. cruzi."} +{"idx": 2000029, "en": "AIMS Type 1 diabetes (T1D) is often associated with early microvascular complications. Previous studies demonstrated that increased systolic (SAP) and diastolic arterial blood pressures (DAP) are linked to microvascular morbidity in T1D. The aim of the study was to investigate the predictive role of neutrophil gelatinase-associated lipocalin (NGAL) in unravelling early cardio-renal dysfunction in T1D. METHODS Two T1D patient groups participating in two-centre prospective cohorts were studied. Group A consisted of 57 participants aged 13.9 years (SD: 3.1) and group B consisted of 59 patients aged 28.0 years (SD: 4.4). Forty-nine healthy children [age: 10.5 years (SD: 6.6)] and 18 healthy adults [age 27.7 years (SD: 4.2)] served as controls. Serum concentrations of NGAL (ELISA) were determined, and SAP and DAP were examined (SAP and DAP also expressed as z-scores in the younger group). RESULTS NGAL correlated positively with SAP in both patient groups (P = 0.020 and P = 0.031, resp.) and SAP z-score (P = 0.009) (group A) and negatively with eGFR in both groups (P < 0.001 and P < 0.001, resp.). CONCLUSIONS NGAL may be proposed as a biomarker of early renal dysfunction even in nonalbuminuric T1D patients, since it was strongly associated with renal function decline and increasing systolic arterial pressure even at prehypertensive range in people with T1D, in a broad age range.", "gemini": "MỤC ĐÍCH Bệnh tiểu đường týp 1 (T1D) thường liên quan đến các biến chứng vi mạch sớm. Các nghiên cứu trước đây đã chứng minh rằng huyết áp động mạch tâm thu (SAP) và huyết áp động mạch tâm trương (DAP) tăng liên quan đến bệnh lý vi mạch ở T1D. Mục đích của nghiên cứu này là điều tra vai trò dự đoán của lipocalin liên quan đến gelatinase của bạch cầu trung tính (NGAL) trong việc làm sáng tỏ rối loạn chức năng tim thận sớm ở T1D. PHƯƠNG PHÁP Hai nhóm bệnh nhân T1D tham gia vào các nhóm nghiên cứu triển vọng hai trung tâm đã được nghiên cứu. Nhóm A bao gồm 57 người tham gia ở độ tuổi 13,9 (SD: 3,1) và nhóm B bao gồm 59 bệnh nhân ở độ tuổi 28,0 (SD: 4,4). Bốn mươi chín trẻ em khỏe mạnh [tuổi: 10,5 (SD: 6,6)] và 18 người lớn khỏe mạnh [tuổi 27,7 (SD: 4,2)] làm đối chứng. Nồng độ NGAL trong huyết thanh (ELISA) đã được xác định và SAP và DAP đã được kiểm tra (SAP và DAP cũng được biểu thị dưới dạng điểm z ở nhóm trẻ hơn). KẾT QUẢ NGAL tương quan dương với SAP ở cả hai nhóm bệnh nhân (P = 0,020 và P = 0,031, tương ứng) và điểm z của SAP (P = 0,009) (nhóm A) và tương quan âm với eGFR ở cả hai nhóm (P < 0,001 và P < 0,001, tương ứng). KẾT LUẬN NGAL có thể được đề xuất như một dấu ấn sinh học của rối loạn chức năng thận sớm ngay cả ở những bệnh nhân T1D không có albumin niệu, vì nó có liên quan chặt chẽ với sự suy giảm chức năng thận và tăng huyết áp tâm thu ngay cả ở phạm vi tăng huyết áp ở những người mắc T1D, trong độ tuổi rộng."} +{"idx": 2000030, "en": "BACKGROUND Matrix-assisted autologous chondrocyte transplantation (MACT) is a surgical treatment option for articular cartilage lesions of the knee joint. PURPOSE To investigate mid- to long-term clinical outcomes of MACT in the patellofemoral (PF) and tibiofemoral (TF) joints. STUDY DESIGN Systematic review; Level of evidence, 4. METHODS A systematic review was performed by searching PubMed, Embase, and the Cochrane Library to find studies evaluating minimum 5-year clinical outcomes of patients undergoing MACT in the knee joint. Search terms used were knee, matrix, and autologous chondrocyte. Patients were evaluated based on treatment failure rates, magnetic resonance imaging, and subjective outcome scores. Study methodology was assessed using the Modified Coleman Methodology Score (MCMS). RESULTS Ten studies (two level 1, one level 2, one level 3, and six level 4 evidence) were identified that met inclusion and exclusion criteria, for a total of 442 TF patients and 136 PF patients. Treatment failure occurred in 9.7% of all patients, including 4.7% of PF patients and 12.4% of TF patients (P = .037). Weighted averages of subjective outcome scores, including Knee injury and Osteoarthritis Outcome Score, Short Form-36 Health Survey, and Tegner scores, improved from baseline to latest follow-up in both TF and PF patients. The mean MCMS was found to be 57.4, with a standard deviation of 18.5. CONCLUSION Patients undergoing MACT in the knee show favorable mid- to long-term clinical outcomes. A significantly higher treatment failure rate was found in patients undergoing MACT in the TF joint compared with the PF joint.", "gemini": "BỐI CẢNH Ghép sụn tự thân hỗ trợ ma trận (MACT) là một lựa chọn điều trị phẫu thuật cho các tổn thương sụn khớp của khớp gối. MỤC ĐÍCH Nghiên cứu kết quả lâm sàng trung hạn đến dài hạn của MACT ở khớp bánh chè đùi (PF) và khớp gối chày (TF). THIẾT KẾ NGHIÊN CỨU Đánh giá có hệ thống; Mức độ bằng chứng, 4. PHƯƠNG PHÁP Đã thực hiện đánh giá có hệ thống bằng cách tìm kiếm PubMed, Embase và Thư viện Cochrane để tìm các nghiên cứu đánh giá kết quả lâm sàng tối thiểu 5 năm của những bệnh nhân trải qua MACT ở khớp gối. Các thuật ngữ tìm kiếm được sử dụng là đầu gối, ma trận và tế bào sụn tự thân. Bệnh nhân được đánh giá dựa trên tỷ lệ thất bại điều trị, chụp cộng hưởng từ và điểm kết quả chủ quan. Phương pháp nghiên cứu được đánh giá bằng cách sử dụng Điểm phương pháp Coleman đã sửa đổi (MCMS). KẾT QUẢ Đã xác định được mười nghiên cứu (hai mức 1, một mức 2, một mức 3 và sáu mức 4 bằng chứng) đáp ứng tiêu chí đưa vào và loại trừ, với tổng số 442 bệnh nhân TF và 136 bệnh nhân PF. Thất bại điều trị xảy ra ở 9,7% trong số tất cả bệnh nhân, bao gồm 4,7% bệnh nhân PF và 12,4% bệnh nhân TF (P = 0,037). Điểm trung bình có trọng số của điểm kết quả chủ quan, bao gồm Điểm kết quả chấn thương đầu gối và thoái hóa khớp, Khảo sát sức khỏe dạng ngắn 36 và điểm Tegner, được cải thiện từ ban đầu đến lần theo dõi gần đây nhất ở cả bệnh nhân TF và PF. Điểm MCMS trung bình được tìm thấy là 57,4, với độ lệch chuẩn là 18,5. KẾT LUẬN Bệnh nhân trải qua MACT ở đầu gối cho thấy kết quả lâm sàng trung hạn đến dài hạn thuận lợi. Tỷ lệ thất bại điều trị cao hơn đáng kể được tìm thấy ở những bệnh nhân trải qua MACT ở khớp TF so với khớp PF."} +{"idx": 2000031, "en": "Sclerosing cholangitis and cholestatic jaundice secondary to metastatic disease is a rare complication. We report a rare case of secondary sclerosing cholangitis (SSC) due to lymphatic spread from ovarian cancer with complete resolution after chemotherapy. The diagnosis of SSC from metastatic ovarian cancer was clinically challenging, as endoscopic retrograde cholangiopancreatography revealed irregular hepatic ducts consistent with sclerosing cholangitis, but it did not identify any malignant cells. The final diagnosis was made with liver biopsy revealing high-grade metastatic Mullerian carcinoma. The patient responded well to chemotherapy and is in remission. A timely diagnosis is important and can lead to complete resolution of the disease.", "gemini": "Viêm đường mật xơ hóa và vàng da ứ mật thứ phát do bệnh di căn là một biến chứng hiếm gặp. Chúng tôi báo cáo một trường hợp hiếm gặp về viêm đường mật xơ hóa thứ phát (SSC) do ung thư buồng trứng lan truyền theo đường bạch huyết với tình trạng phục hồi hoàn toàn sau hóa trị. Việc chẩn đoán SSC từ ung thư buồng trứng di căn là một thách thức về mặt lâm sàng, vì chụp ngược dòng mật tụy nội soi cho thấy các ống gan không đều phù hợp với viêm đường mật xơ hóa, nhưng không phát hiện thấy bất kỳ tế bào ác tính nào. Chẩn đoán cuối cùng được đưa ra bằng sinh thiết gan cho thấy ung thư biểu mô Mullerian di căn cấp độ cao. Bệnh nhân đáp ứng tốt với hóa trị và đang trong thời gian thuyên giảm. Chẩn đoán kịp thời rất quan trọng và có thể dẫn đến phục hồi hoàn toàn căn bệnh."} +{"idx": 2000032, "en": "We analyzed hematopoietic phenotypes in Peromyscus leucopus (PL) mice at young (2-9 months), middle (22-23 months) and old (33-46 months) ages aimed at characterizing age-associated changes in this unique rodent species. We found a significantly higher number of monocytes in old PL mice in peripheral blood, and higher proportions of CD44+ cells in blood, spleen and bone marrow in old PL mice than in middle and young counterparts. We conclude that elevated blood monocyte counts and up-regulated hematopoietic cell CD44 expression are two useful aging biomarkers for PL mice.", "gemini": "Chúng tôi đã phân tích kiểu hình tạo máu ở chuột Peromyscus leucopus (PL) ở độ tuổi trẻ (2-9 tháng), trung niên (22-23 tháng) và già (33-46 tháng) nhằm mục đích mô tả những thay đổi liên quan đến tuổi ở loài gặm nhấm độc đáo này. Chúng tôi phát hiện ra số lượng tế bào đơn nhân cao hơn đáng kể ở chuột PL già trong máu ngoại vi và tỷ lệ tế bào CD44+ cao hơn trong máu, lá lách và tủy xương ở chuột PL già so với các đối tượng trung niên và trẻ. Chúng tôi kết luận rằng số lượng tế bào đơn nhân trong máu tăng cao và biểu hiện CD44 của tế bào tạo máu được điều chỉnh tăng lên là hai dấu ấn sinh học lão hóa hữu ích đối với chuột PL."} +{"idx": 2000033, "en": "Cytosolic Ca2+ is a universal second messenger that is involved in many processes throughout the body, including the regulation of cell growth/cell division, apoptosis, and the secretion of both ions, and macromolecules. Tobacco smoke exerts multiple effects on airway epithelia and we have previously shown that Kentucky reference cigarette smoke exposure elevated the second messenger Ca2+, leading to dysfunctional ion secretion. In this study, we tested whether little cigar and commercial cigarette smoke exposure exerts similar effects on intracellular Ca2+ levels. Indeed, Swisher Sweets, Captain Black, and Cheyenne little cigars, as well as Camel, Marlboro, and Newport cigarettes, triggered a comparable increase in intracellular Ca2+ as seen with Kentucky reference cigarettes in human bronchial epithelia. We also found that Kentucky reference cigarette smoke exposure caused increases in Ca2+ in HEK293T cells and that similar increases in Ca2+ were seen with the tobacco smoke metabolites 1-NH2-naphthalene, formaldehyde, nicotine, and nicotine-derived nitrosamine ketone. Given the large number of physiological processes governed by changes in cytosolic Ca2+, our data suggest that Ca2+ signaling is a useful and reproducible assay that can be used to probe the propensity of tobacco products and their constituents to cause toxicity.", "gemini": "Cytosolic Ca2+ là một chất truyền tin thứ hai phổ biến liên quan đến nhiều quá trình trên khắp cơ thể, bao gồm điều hòa sự tăng trưởng tế bào/phân chia tế bào, quá trình chết rụng tế bào theo chương trình và quá trình tiết ra cả ion và đại phân tử. Khói thuốc lá gây ra nhiều tác động lên biểu mô đường thở và trước đây chúng tôi đã chỉ ra rằng việc tiếp xúc với khói thuốc lá tham chiếu Kentucky làm tăng chất truyền tin thứ hai Ca2+, dẫn đến quá trình tiết ion rối loạn chức năng. Trong nghiên cứu này, chúng tôi đã kiểm tra xem khói thuốc lá xì gà nhỏ và thương mại có gây ra những tác động tương tự đối với nồng độ Ca2+ trong tế bào hay không. Thật vậy, xì gà nhỏ Swisher Sweets, Captain Black và Cheyenne, cũng như thuốc lá Camel, Marlboro và Newport, đã kích hoạt sự gia tăng tương đương Ca2+ trong tế bào như khi thấy với thuốc lá tham chiếu Kentucky trong biểu mô phế quản của người. Chúng tôi cũng phát hiện ra rằng việc tiếp xúc với khói thuốc lá tham chiếu Kentucky gây ra sự gia tăng Ca2+ trong các tế bào HEK293T và sự gia tăng tương tự Ca2+ cũng được thấy với các chất chuyển hóa khói thuốc lá là 1-NH2-naphthalene, formaldehyde, nicotine và nicotine-dẫn xuất nitrosamine ketone. Với số lượng lớn các quá trình sinh lý được điều chỉnh bởi những thay đổi trong Ca2+ tế bào chất, dữ liệu của chúng tôi cho thấy rằng tín hiệu Ca2+ là một xét nghiệm hữu ích và có thể tái tạo được, có thể được sử dụng để thăm dò xu hướng của các sản phẩm thuốc lá và các thành phần của chúng gây ra độc tính."} +{"idx": 2000034, "en": "Introduction: Given the known association between sugar-sweetened beverage (SSB) intake and poorer health, we instituted an educational curriculum to reduce student consumption of SSBs. Methods: The program included third- to fifth-grade students. A simple demonstration using teaspoons of sugar or small candies showed students the quantity of added sugar in common beverages. This amount of sugar was compared to the daily limit recommended by the American Heart Association. Key principles were reinforced over a 4-month period. Anonymous beverage recall surveys were distributed to 213 students at baseline and 211 students 6 months after exposure to the curriculum. Primary endpoints included evaluation of SSB, real fruit juice (RFJ), diet soda, and water servings in the last 24 hours. Results: The proportion of children consuming 2 or more beverages daily decreased from 8.9% to 4.3% (P = .0546) for diet soda, from 70.0% to 58.3% (P = .0123) for SSB + RFJ, and from 60.1% to 47.4% (P = .0087) for SSB. At baseline, students reported an average consumption of 3.5 SSB, 4.5 SSB + RFJ, 0.4 diet soda, and 3.3 water servings per day. At 6 months after exposure, the average daily beverage consumption decreased to 2.7 servings per day for SSB (P = .014), 3.8 for SSB + RFJ (P = .039), and 0.2 for diet soda (P = .027). Water consumption increased from 3.3 to 3.6 servings per day (P = .075). Discussion: Our data suggest grade school students are receptive to information about the adverse effects of SSBs on health. Adding similar educational programs to elementary school curriculum may help reduce long-term SSB consumption.", "gemini": "Mở đầu: Với mối liên quan đã biết giữa việc tiêu thụ đồ uống có đường (SSB) và sức khỏe kém hơn, chúng tôi đã thiết lập một chương trình giáo dục để giảm lượng SSB tiêu thụ của học sinh. Phương pháp: Chương trình bao gồm học sinh từ lớp ba đến lớp năm. Một cuộc trình diễn đơn giản sử dụng thìa đường hoặc kẹo nhỏ cho học sinh thấy lượng đường bổ sung trong các loại đồ uống thông thường. Lượng đường này được so sánh với giới hạn hàng ngày do Hiệp hội Tim mạch Hoa Kỳ khuyến nghị. Các nguyên tắc chính được củng cố trong khoảng thời gian 4 tháng. Các cuộc khảo sát thu hồi đồ uống ẩn danh đã được phân phát cho 213 học sinh lúc ban đầu và 211 học sinh sau 6 tháng tiếp xúc với chương trình giảng dạy. Các điểm cuối chính bao gồm đánh giá SSB, nước ép trái cây thật (RFJ), nước ngọt ăn kiêng và khẩu phần nước trong 24 giờ qua. Kết quả: Tỷ lệ trẻ em tiêu thụ 2 hoặc nhiều đồ uống mỗi ngày giảm từ 8,9% xuống 4,3% (P = .0546) đối với nước ngọt ăn kiêng, từ 70,0% xuống 58,3% (P = .0123) đối với SSB + RFJ và từ 60,1% xuống 47,4% (P = .0087) đối với SSB. Khi bắt đầu, học sinh báo cáo mức tiêu thụ trung bình là 3,5 SSB, 4,5 SSB + RFJ, 0,4 nước ngọt ăn kiêng và 3,3 khẩu phần nước mỗi ngày. Sau 6 tháng tiếp xúc, mức tiêu thụ đồ uống trung bình hàng ngày giảm xuống còn 2,7 khẩu phần mỗi ngày đối với SSB (P = .014), 3,8 đối với SSB + RFJ (P = .039) và 0,2 đối với nước ngọt ăn kiêng (P = .027). Lượng nước tiêu thụ tăng từ 3,3 lên 3,6 khẩu phần mỗi ngày (P = .075). Thảo luận: Dữ liệu của chúng tôi cho thấy học sinh tiểu học tiếp thu thông tin về những tác động xấu của SSB đối với sức khỏe. Việc bổ sung các chương trình giáo dục tương tự vào chương trình giảng dạy của trường tiểu học có thể giúp giảm lượng SSB tiêu thụ trong thời gian dài."} +{"idx": 2000035, "en": "Pediatric hypertension is a risk for adult cardiovascular disease, making early detection important. The prevalence of pediatric essential hypertension is rising due to the increased prevalence of obesity. Though guidelines for screening, diagnosis, evaluation, and management are available, there are barriers to accurate diagnosis of pediatric hypertension, including lack of knowledge and complexity of blood pressure standards. We aimed to gain insights into reasons for low rates of diagnosis and treatment from primary care providers. As part of a multisite randomized controlled trial, we interviewed 8 providers in a community health center network. We used a grounded theoretical approach to analyze transcripts. Providers reflected on numerous barriers to diagnosis, management, and follow-up; recommendations for educational content; and how community health center systems can be improved. Findings informed development of a multifaceted intervention. Despite lack of training on essential hypertension, providers were comfortable recommending lifestyle changes to promote healthier weight and reduced blood pressure.", "gemini": "Tăng huyết áp ở trẻ em là nguy cơ mắc bệnh tim mạch ở người lớn, vì vậy việc phát hiện sớm rất quan trọng. Tỷ lệ tăng huyết áp vô căn ở trẻ em đang gia tăng do tình trạng béo phì ngày càng phổ biến. Mặc dù có hướng dẫn về sàng lọc, chẩn đoán, đánh giá và điều trị, nhưng vẫn có những rào cản đối với việc chẩn đoán chính xác tăng huyết áp ở trẻ em, bao gồm việc thiếu kiến thức và sự phức tạp của các tiêu chuẩn huyết áp. Chúng tôi đã đặt mục tiêu tìm hiểu những lý do khiến tỷ lệ chẩn đoán và điều trị thấp từ các nhà cung cấp dịch vụ chăm sóc chính. Là một phần của thử nghiệm đối chứng ngẫu nhiên đa địa điểm, chúng tôi đã phỏng vấn 8 nhà cung cấp dịch vụ trong một mạng lưới trung tâm y tế cộng đồng. Chúng tôi đã sử dụng phương pháp lý thuyết cơ bản để phân tích các bản ghi. Các nhà cung cấp đã phản ánh về nhiều rào cản đối với việc chẩn đoán, quản lý và theo dõi; các khuyến nghị về nội dung giáo dục; và làm thế nào để cải thiện các hệ thống trung tâm y tế cộng đồng. Những phát hiện này đã cung cấp thông tin để phát triển một biện pháp can thiệp đa phương diện. Mặc dù thiếu đào tạo về tăng huyết áp vô căn, nhưng các nhà cung cấp cảm thấy thoải mái khi khuyến nghị thay đổi lối sống để thúc đẩy cân nặng khỏe mạnh hơn và giảm huyết áp."} +{"idx": 2000036, "en": "Oromandibular dystonia (OMD) is a rare focal neurological disorder that affects mouth, face, and jaws. This comprehensive literature review aimed to summarize the current evidence for etiology, diagnosis, and management of OMD and assess the possibility of dental origin of the disease and dental treatment plans for these patients. Different online databases namely PubMed, Google scholar, and Scopus were searched. The keywords \"oromandibular dystonia\", \"orofaciomandibular dystonia\", \"orofacial-buccal dystonia\", \"lingual dystonia\", \"jaw dystonia\", \"cranial dystonia\", and \"adult-onset facial dystonia\" were searched in the title and abstract of publications from 1970 to 2016. The inclusion criterion was the dental etiology and/or dental treatment. Out of 1260 articles, only 37 articles met the inclusion criteria. OMD can be caused or exacerbated through different dental treatments within which anyone is likely to be involved due to various reasons. Some novel methods employed to relieve this syndrome have led to certain cure or improvement of symptoms in several cases. OMD patients may refer to dentists with involuntary jaw movements and intraoral presentations. Thus, the dentists should be aware of the symptoms and signs and refer the suspicious cases. Dentists should also be familiar with special considerations when managing OMD patients.", "gemini": "Rối loạn trương lực cơ hàm mặt (OMD) là một rối loạn thần kinh cục bộ hiếm gặp ảnh hưởng đến miệng, mặt và hàm. Mục đích của tổng quan tài liệu toàn diện này là tóm tắt các bằng chứng hiện tại về bệnh nguyên, chẩn đoán và xử trí OMD và đánh giá khả năng bệnh có nguồn gốc từ răng và các kế hoạch điều trị nha khoa cho những bệnh nhân này. Nhiều cơ sở dữ liệu trực tuyến khác nhau cụ thể là PubMed, Google Scholar và Scopus đã được tìm kiếm. Các từ khóa \"rối loạn trương lực cơ hàm mặt\", \"rối loạn trương lực cơ hàm mặt\", \"rối loạn trương lực cơ hàm mặt\", \"rối loạn trương lực cơ lưỡi\", \"rối loạn trương lực cơ hàm\", \"rối loạn trương lực cơ sọ\" và \"rối loạn trương lực cơ mặt khởi phát ở người lớn\" đã được tìm kiếm trong tiêu đề và tóm tắt các ấn phẩm từ năm 1970 đến năm 2016. Tiêu chuẩn đưa vào là bệnh nguyên về răng và/hoặc điều trị nha khoa. Trong số 1260 bài báo, chỉ có 37 bài báo đáp ứng các tiêu chuẩn đưa vào. OMD có thể do nhiều phương pháp điều trị nha khoa khác nhau gây ra hoặc làm trầm trọng thêm, trong đó bất kỳ ai cũng có khả năng bị ảnh hưởng do nhiều lý do khác nhau. Một số phương pháp mới được sử dụng để làm giảm hội chứng này đã dẫn đến việc chữa khỏi hoặc cải thiện các triệu chứng ở một số trường hợp. Bệnh nhân OMD có thể gặp nha sĩ với các cử động hàm không tự chủ và biểu hiện trong miệng. Do đó, nha sĩ cần nhận biết các triệu chứng và dấu hiệu và giới thiệu các trường hợp nghi ngờ. Nha sĩ cũng cần nắm rõ các cân nhắc đặc biệt khi xử trí bệnh nhân OMD."} +{"idx": 2000037, "en": "STATEMENT OF THE PROBLEM The bond strength of composites with different adhesive systems with dentin is an important factor in long term durability of composite restorations. The effect of titanium tetrafluoride (TiF4) as anti caries agent and sodium hypochlorite (NaOCl) as disinfectant on the shear bond of nanofilled and silorane based composite resins have not been investigated in previous studies. PURPOSE This study was conducted to determine bond strength between dentin and two composite systems, by means of shear bond test using TiF4 and NaOCl. MATERIALS AND METHOD Middle dentin of 60 intact extracted maxillary premolar teeth were exposed by sectioning the crowns at a depth of 2mm from central groove and parallel to the occlusal surface. Standardized smear layer was created using a 600-grit silicon carbide paper and then samples were embedded in acrylic resin blocks. Then the samples were randomly divided into 6 \\groups summarized as Group I: Z350, Group II: Z350+ NaOCl, Group III: Z350+ TiF4, Group IV: P90, Group V: P90+ NaOCl, Group VI: P90+ TiF4 according to manufacturer's instruction. Then samples were subjected to shear bond strength (SBS) test using universal testing machine and data were analyzed using ANOVA and Tukey tests (p< 0.05). RESULTS Application of 5% NaOCl caused a significant decrease in SBS of nanofilled composite resin (p= 0.004), and also silorane based composite resin (p= 0.006). Application of 4% TiF4 caused a significant increase in SBS of silorane based composite resin (p= 0.001). The effect of TiF4 on nanofilled composite was not statistically significant. CONCLUSION Using TiF4 has a positive effect on increasing the shear bond while NaOCl has negative effect on bond strength.", "gemini": "TÓM TẮT VẤN ĐỀ Độ bền liên kết của vật liệu composite với các hệ thống kết dính khác nhau với ngà răng là một yếu tố quan trọng trong độ bền lâu dài của phục hình composite. Tác dụng của titan tetrafluoride (TiF4) như một tác nhân chống sâu răng và natri hypochlorite (NaOCl) như một chất khử trùng đối với liên kết cắt của nhựa composite có chứa nano và dựa trên siloran chưa được nghiên cứu trong các nghiên cứu trước đây. MỤC ĐÍCH Nghiên cứu này được tiến hành để xác định độ bền liên kết giữa ngà răng và hai hệ thống composite, bằng phương pháp kiểm tra liên kết cắt sử dụng TiF4 và NaOCl. VẬT LIỆU VÀ PHƯƠNG PHÁP Ngà răng giữa của 60 răng tiền hàm trên đã nhổ nguyên vẹn được lộ ra bằng cách cắt thân răng ở độ sâu 2mm tính từ rãnh trung tâm và song song với mặt nhai. Lớp bôi bẩn chuẩn hóa được tạo ra bằng cách sử dụng giấy silicon carbide có độ nhám 600, sau đó các mẫu vật được nhúng vào các khối nhựa acrylic. Sau đó, các mẫu vật được chia ngẫu nhiên thành 6 nhóm được tóm tắt như Nhóm I: Z350, Nhóm II: Z350+ NaOCl, Nhóm III: Z350+ TiF4, Nhóm IV: P90, Nhóm V: P90+ NaOCl, Nhóm VI: P90+ TiF4 theo hướng dẫn của nhà sản xuất. Sau đó, các mẫu vật được thử nghiệm độ bền liên kết cắt (SBS) bằng máy thử vạn năng và dữ liệu được phân tích bằng các phép thử ANOVA và Tukey (p< 0,05). KẾT QUẢ Việc sử dụng 5% NaOCl gây giảm đáng kể SBS của nhựa composite có chứa nano (p= 0,004) và cả nhựa composite dựa trên siloran (p= 0,006). Việc sử dụng 4% TiF4 làm tăng đáng kể SBS của nhựa composite dựa trên siloran (p= 0,001). Tác dụng của TiF4 đối với composite có chứa nano không có ý nghĩa thống kê. KẾT LUẬN Sử dụng TiF4 có tác dụng tích cực trong việc tăng liên kết cắt trong khi NaOCl có tác dụng tiêu cực đến độ bền liên kết."} +{"idx": 2000038, "en": "Perceptions of people living with chronic illness change over time, contributing to health-related stress that necessitates coping skills. Paterson's shifting perspectives model provides an explanation of chronically ill people's variations in attention to their symptoms. In this qualitative study, 20 people with fibromyalgia living in a rural setting were interviewed in 2013 with the aim of gaining insight into their experiences and the meaning-making associated with their chronic condition. Analysis of the interview data categorized five recurrent, or common, themes: experiences of loss, feelings of fear and uncertainty, influence of stress, stigmatization of the disease, and coping through courage. Difficulties attendant to losses, distress, and stigma associated with this chronic condition led the participants to report poor health-related quality of life. The study findings can be useful across clinical settings to nurses and other health care providers in understanding those diagnosed with fibromyalgia and their care needs.", "gemini": "Nhận thức của những người sống chung với bệnh mãn tính thay đổi theo thời gian, góp phần gây ra căng thẳng liên quan đến sức khỏe, đòi hỏi phải có các kỹ năng đối phó. Mô hình quan điểm thay đổi của Paterson đưa ra lời giải thích về sự thay đổi trong việc chú ý đến các triệu chứng của những người mắc bệnh mãn tính. Trong nghiên cứu định tính này, 20 người mắc bệnh đau xơ cơ sống ở vùng nông thôn đã được phỏng vấn vào năm 2013 với mục đích tìm hiểu sâu hơn về kinh nghiệm và ý nghĩa liên quan đến tình trạng mãn tính của họ. Phân tích dữ liệu phỏng vấn đã phân loại năm chủ đề phổ biến hoặc thường gặp: trải nghiệm mất mát, cảm giác sợ hãi và không chắc chắn, ảnh hưởng của căng thẳng, kỳ thị bệnh tật và đối phó bằng lòng can đảm. Những khó khăn liên quan đến mất mát, đau khổ và kỳ thị liên quan đến tình trạng mãn tính này khiến những người tham gia báo cáo chất lượng cuộc sống liên quan đến sức khỏe kém. Các phát hiện của nghiên cứu có thể hữu ích trong các bối cảnh lâm sàng đối với y tá và các nhà cung cấp dịch vụ chăm sóc sức khỏe khác trong việc hiểu những người được chẩn đoán mắc bệnh đau xơ cơ và nhu cầu chăm sóc của họ."} +{"idx": 2000039, "en": "STATEMENT OF THE PROBLEM Most of the soft tissue calcifications within the head and neck region might not be accompanied by clinical symptoms but may indicate some pathological conditions. PURPOSE The aim of this research was to determine the prevalence of soft tissue calcifications in cone beam computed tomography (CBCT) images of mandibular region. MATERIALS AND METHOD In this cross sectional study the CBCT images of 602 patients including 294 men and 308 women with mean age 41.38±15.18 years were evaluated regarding the presence, anatomical location; type (single or multiple) and size of soft tissue calcification in mandibular region. All CBCT images were acquired by NewTom VGi scanner. Odds ratio and chi-square tests were used for data analysis and p< 0.05 was considered to be statistically significant. RESULTS 156 out of 602 patients had at least one soft tissue calcification in their mandibular region (25.9%. of studied population with mean age 51.7±18.03 years). Men showed significantly higher rate of soft tissue calcification than women (30.3% vs. 21.8%). Soft tissue calcification was predominantly seen at posterior region of the mandible (88%) and most of them were single (60.7%). The prevalence of soft tissue calcification increased with age. Most of the detected soft tissue calcifications were smaller than 3mm (90%). CONCLUSION Soft tissue calcifications in mandibular area were a relatively common finding especially in posterior region and more likely to happen in men and in older age group.", "gemini": "TÓM TẮT VẤN ĐỀ Hầu hết các canxi hóa mô mềm trong vùng đầu và cổ có thể không đi kèm với các triệu chứng lâm sàng nhưng có thể biểu hiện một số tình trạng bệnh lý. MỤC ĐÍCH Mục đích của nghiên cứu này là xác định tỷ lệ mắc canxi hóa mô mềm trong hình ảnh chụp cắt lớp chùm tia hình nón (CBCT) của vùng xương hàm. VẬT LIỆU VÀ PHƯƠNG PHÁP Trong nghiên cứu cắt ngang này, các hình ảnh CBCT của 602 bệnh nhân bao gồm 294 nam và 308 nữ với độ tuổi trung bình là 41,38 ± 15,18 tuổi đã được đánh giá về sự hiện diện, vị trí giải phẫu; loại (đơn hoặc nhiều) và kích thước của canxi hóa mô mềm ở vùng xương hàm. Tất cả các hình ảnh CBCT đều được chụp bằng máy quét NewTom VGi. Tỷ lệ cược và kiểm định chi bình phương được sử dụng để phân tích dữ liệu và p < 0,05 được coi là có ý nghĩa thống kê. KẾT QUẢ 156 trong số 602 bệnh nhân có ít nhất một canxi hóa mô mềm ở vùng xương hàm (25,9%. dân số được nghiên cứu với độ tuổi trung bình là 51,7 ± 18,03 tuổi). Nam giới có tỷ lệ canxi hóa mô mềm cao hơn đáng kể so với nữ giới (30,3% so với 21,8%). Canxi hóa mô mềm chủ yếu được nhìn thấy ở vùng sau của xương hàm (88%) và hầu hết trong số chúng là đơn lẻ (60,7%). Tỷ lệ mắc canxi hóa mô mềm tăng theo tuổi. Hầu hết các canxi hóa mô mềm được phát hiện có kích thước nhỏ hơn 3mm (90%). KẾT LUẬN Canxi hóa mô mềm ở vùng xương hàm là một phát hiện tương đối phổ biến đặc biệt ở vùng sau và có nhiều khả năng xảy ra ở nam giới và nhóm tuổi lớn hơn."} +{"idx": 2000040, "en": "STATEMENT OF THE PROBLEM Recently allogenic mesenchymal stem cells are proposed to have multipotential progenitor cell capabilities to differentiate into cementoblasts, osteoblasts, and periodontal ligament fibroblasts. PURPOSE The aim of the present study was to compare the efficacy of human umbilical stem cells cultured on polylactic acid (PLA), polyglycolic acid (PGA) membrane with PLA/PGA membrane alone in the treatment of multiple gingival recession defects. MATERIALS AND METHOD A total number of 14 cases of multiple gingival recession (Miller's Class I or II) located in the anterior region were randomly selected and divided into test (stem cells in combination with PLA/PGA membrane) and control group (PLA/PGA membrane alone). Clinical parameters including gingival recession, probing pocket depth, clinical attachment level, and width of keratinized gingiva were recorded at baseline, and at 6 months postoperative. RESULTS At baseline, there was 2.28 mm and 2.14mm mean gingival recession at 16 sites and 14 sites in test and control groups respectively. At 6 months post-surgery, test group showed 1.57 mm mean reduction of gingival recession indicating 66% root coverage, while the control group showed 1.24mm mean reduction of gingival recession indicating 57% root coverage. CONCLUSION In the present study, the stem cell with PLA/PGA membrane showed significantly higher mean root coverage compared to only PLA/PGA membrane group.", "gemini": "TÓM TẮT VẤN ĐỀ Gần đây, các tế bào gốc trung mô dị loại được đề xuất có khả năng tiền thân đa năng để biệt hóa thành nguyên bào xi măng, nguyên bào xương và nguyên bào sợi dây chằng nha chu. MỤC ĐÍCH Mục đích của nghiên cứu hiện tại là so sánh hiệu quả của các tế bào gốc dây rốn người nuôi cấy trên màng axit polylactic (PLA), màng axit polyglycolic (PGA) với chỉ màng PLA/PGA trong điều trị nhiều khuyết hổng tụt nướu. VẬT LIỆU VÀ PHƯƠNG PHÁP Tổng cộng 14 trường hợp tụt nướu nhiều (Miller loại I hoặc II) nằm ở vùng trước được chọn ngẫu nhiên và chia thành nhóm thử nghiệm (tế bào gốc kết hợp với màng PLA/PGA) và nhóm đối chứng (chỉ màng PLA/PGA). Các thông số lâm sàng bao gồm tụt nướu, độ sâu túi thăm dò, mức bám dính lâm sàng và chiều rộng nướu đã sừng hóa được ghi lại tại thời điểm ban đầu và sau 6 tháng phẫu thuật. KẾT QUẢ Vào thời điểm ban đầu, có 2,28 mm và 2,14 mm tụt nướu trung bình tại 16 vị trí và 14 vị trí ở nhóm thử nghiệm và nhóm đối chứng tương ứng. Sau 6 tháng phẫu thuật, nhóm thử nghiệm cho thấy giảm trung bình 1,57 mm tụt nướu cho thấy che phủ chân răng 66%, trong khi nhóm đối chứng cho thấy giảm trung bình 1,24 mm tụt nướu cho thấy che phủ chân răng 57%. KẾT LUẬN Trong nghiên cứu hiện tại, tế bào gốc với màng PLA/PGA cho thấy độ che phủ chân răng trung bình cao hơn đáng kể so với chỉ nhóm màng PLA/PGA."} +{"idx": 2000041, "en": "STATEMENT OF THE PROBLEM Dental caries in infants and young children is prevalent worldwide; its magnitude and associated factors vary between communities because of cultural and social economic differences. No such information was available for pre-school children in Mbeya city. PURPOSE To determine dental caries status and associated factors in 2 to 4-year-old children in Mbeya city. MATERIALS AND METHOD A cross sectional study was conducted among 525 children aged 2-4 years and their parents/caregivers. Caries was assessed using caries assessment spectrum and treatment index, oral hygiene by visual inspection for visible plaque on index teeth, and dietary and oral hygiene habits by a questionnaire. Kappa statistics was used to test reliability of study instruments, χ2-test and logistic regression was employed for studying associations. RESULTS Caries free children for dmft1, dmft2 and dmft3 were 79.8%, 83.8% and 94.7% and caries experience was 0.49 (1.23), 0.4 (1.14) and 0.10 (0.53) respectively. Older age [(OR =2.722 (1.617-4.582) p=< 0.001)]; and frequent consumption of factory made sugary foods/snacks at age 1-2 years [(OR=3.061 (1.188-7.887) p=0.021] were associated with caries. Prolonged breastfeeding for more than 1 year and breastfeeding at night had no association with dental caries. CONCLUSION The prevalence of dental caries was very low. Older age and frequent consumption of factory made sugary foods at age 1-2 years were associated with higher odds of developing dental caries. Prolonged breasfeeding and breastfeeding at night had no association with dental caries. Prevention of dental caries should be instituted as soon as primary teeth start erupting, especially through discouraging consumption of factory made sugary foods/snacks.", "gemini": "TÓM TẮT VẤN ĐỀ Sâu răng ở trẻ sơ sinh và trẻ nhỏ rất phổ biến trên toàn thế giới; mức độ và các yếu tố liên quan khác nhau giữa các cộng đồng do sự khác biệt về văn hóa và kinh tế xã hội. Không có thông tin nào như vậy đối với trẻ mẫu giáo ở thành phố Mbeya. MỤC ĐÍCH Xác định tình trạng sâu răng và các yếu tố liên quan ở trẻ em từ 2 đến 4 tuổi ở thành phố Mbeya. VẬT LIỆU VÀ PHƯƠNG PHÁP Một nghiên cứu cắt ngang đã được tiến hành trên 525 trẻ em từ 2-4 tuổi và cha mẹ/người chăm sóc của chúng. Sâu răng được đánh giá bằng phổ đánh giá sâu răng và chỉ số điều trị, vệ sinh răng miệng bằng cách kiểm tra trực quan mảng bám có thể nhìn thấy trên răng chỉ số và thói quen vệ sinh răng miệng và chế độ ăn uống bằng bảng câu hỏi. Thống kê Kappa được sử dụng để kiểm tra độ tin cậy của các công cụ nghiên cứu, kiểm định χ2 và hồi quy logistic được sử dụng để nghiên cứu các mối liên quan. KẾT QUẢ Trẻ em không sâu răng đối với dmft1, dmft2 và dmft3 lần lượt là 79,8%, 83,8% và 94,7% và kinh nghiệm sâu răng lần lượt là 0,49 (1,23), 0,4 (1,14) và 0,10 (0,53). Trẻ lớn tuổi [(OR = 2,722 (1,617-4,582) p = <0,001)]; và thường xuyên tiêu thụ thực phẩm/đồ ăn nhẹ có đường do nhà máy sản xuất ở độ tuổi 1-2 [(OR = 3,061 (1,188-7,887) p = 0,021] có liên quan đến sâu răng. Cho con bú kéo dài hơn 1 năm và cho con bú vào ban đêm không liên quan đến sâu răng. KẾT LUẬN Tỷ lệ sâu răng rất thấp. Trẻ lớn tuổi và thường xuyên tiêu thụ thực phẩm có đường do nhà máy sản xuất ở độ tuổi 1-2 có tỷ lệ mắc sâu răng cao hơn. Cho con bú kéo dài và cho con bú vào ban đêm không liên quan đến sâu răng. Cần thiết lập biện pháp phòng ngừa sâu răng ngay khi răng sữa bắt đầu mọc, đặc biệt là thông qua việc không khuyến khích tiêu thụ thực phẩm/đồ ăn nhẹ có đường do nhà máy sản xuất."} +{"idx": 2000042, "en": "STATEMENT OF THE PROBLEM Hearing impaired children have a problem in understanding and comprehending with dental treatments. Visual language is the sensible answer of how to improve communication with them. PURPOSE To evaluate the applicability of dental sign language in Hearing impaired children in relieving anxiety during stressful dental treatment by improving their means of communication. MATERIALS AND METHOD This randomized clinical trial was carried out in the Department of Pedodontics and Preventive Dentistry which included 40 Hearing Impaired children meeting inclusion criteria. The selected children were randomly divided into the study and control group comprising of 20 each. In the control group, initial oral examination and dental treatment (oral prophylaxis and class I restoration) were performed without the use of dental sign language. In the study group, the dental sign language specific to dental treatment was educated and during their subsequent visit to the dental clinic after dental sign language reinforcement, oral prophylaxis and class I restoration were done. Subjective and objective measurements of anxiety were recorded for both groups using facial image scale (FIS), pulse oximeter and electronic blood pressure apparatus to compare for correlation. The obtained data were subjected to statistical analysis using unpaired t-test. RESULTS There was a statistically significant reduction in the anxiety levels (p< 0.05) in the study group compared to the control group. CONCLUSION Dental sign language was effective in reducing the level of anxiety in children who are hard of hearing. Dental sign language was able to improve behavior positively during dental treatment and may also aid in developing a positive dental attitude among children who are hard of hearing.", "gemini": "TÓM TẮT VẤN ĐỀ Trẻ em khiếm thính gặp vấn đề trong việc hiểu và tiếp thu các phương pháp điều trị nha khoa. Ngôn ngữ hình ảnh là giải pháp hợp lý để cải thiện giao tiếp với trẻ em khiếm thính. MỤC ĐÍCH Đánh giá tính ứng dụng của ngôn ngữ ký hiệu nha khoa ở trẻ em khiếm thính trong việc giảm lo lắng trong quá trình điều trị nha khoa căng thẳng bằng cách cải thiện phương tiện giao tiếp của trẻ. VẬT LIỆU VÀ PHƯƠNG PHÁP Thử nghiệm lâm sàng ngẫu nhiên này được thực hiện tại Khoa Nha khoa Nhi và Nha khoa Dự phòng bao gồm 40 trẻ em khiếm thính đáp ứng tiêu chuẩn tuyển chọn. Những trẻ được chọn được chia ngẫu nhiên thành nhóm nghiên cứu và nhóm đối chứng, mỗi nhóm gồm 20 trẻ. Ở nhóm đối chứng, khám miệng ban đầu và điều trị nha khoa (vệ sinh răng miệng và phục hồi lớp I) được thực hiện mà không sử dụng ngôn ngữ ký hiệu nha khoa. Ở nhóm nghiên cứu, ngôn ngữ ký hiệu nha khoa dành riêng cho điều trị nha khoa đã được giáo dục và trong lần tái khám tiếp theo tại phòng khám nha khoa sau khi củng cố ngôn ngữ ký hiệu nha khoa, vệ sinh răng miệng và phục hồi lớp I đã được thực hiện. Các phép đo chủ quan và khách quan về lo lắng đã được ghi lại cho cả hai nhóm bằng cách sử dụng thang hình ảnh khuôn mặt (FIS), máy đo oxy xung và máy đo huyết áp điện tử để so sánh mối tương quan. Dữ liệu thu được đã được phân tích thống kê bằng cách sử dụng kiểm định t không ghép đôi. KẾT QUẢ Có sự giảm đáng kể về mặt thống kê ở mức độ lo lắng (p < 0,05) ở nhóm nghiên cứu so với nhóm đối chứng. KẾT LUẬN Ngôn ngữ ký hiệu nha khoa có hiệu quả trong việc giảm mức độ lo lắng ở trẻ em khiếm thính. Ngôn ngữ ký hiệu nha khoa có thể cải thiện hành vi tích cực trong quá trình điều trị nha khoa và cũng có thể giúp phát triển thái độ nha khoa tích cực ở trẻ em khiếm thính."} +{"idx": 2000043, "en": "STATEMENT OF THE PROBLEM Root canal therapy should not simply be the extirpation of the pulp and widening of the canal. But one should also focus on how to completely remove the loosely-attached smear layer because it has adverse effects on the final outcome of the treatment. PURPOSE This study compared the efficacy of Etidronic acid, SmearClear and MTAD to remove the smear layer created during instrumentation in different regions of the root canal. MATERIALS AND METHOD Fifty single-rooted mandibular premolars were decoronated from the cementoenamel junction and instrumented using the ProTaper universal rotary file system along with copious irrigation by 1.0% sodium hypochlorite and distilled water. On the basis of the type of chelating agent used for irrigation, samples (n=10) were then randomized into five groups as: Group I- 9% etidronic acid, Group II- 18% etidronic acid, Group III- SmearClear, Group IV- MTAD and Group V- normal saline. Subsequent to irrigation, all samples were rinsed, dried and sectioned longitudinally for evaluation of the smear layer removal under scanning electron microscope (2000X). Data were statistically analyzed by two-way analysis of variance and Tukey's post hoc test with statistical significance set at p< 0.5. RESULTS The result showed that SmearClear was the most efficient in removing the smear layer. However, etidronic acid was found inferior than both SmearClear and MTAD. CONCLUSION Chelators are essential for complete smear layer removal in association with organic solvent.", "gemini": "TÓM TẮT VẤN ĐỀ Liệu pháp điều trị tủy không chỉ đơn thuần là cắt bỏ tủy và nới rộng ống tủy. Nhưng người ta cũng nên tập trung vào cách loại bỏ hoàn toàn lớp bôi trơn bám lỏng lẻo vì nó có tác động xấu đến kết quả cuối cùng của quá trình điều trị. MỤC ĐÍCH Nghiên cứu này so sánh hiệu quả của axit Etidronic, SmearClear và MTAD trong việc loại bỏ lớp bôi trơn được tạo ra trong quá trình dụng cụ ở các vùng khác nhau của ống tủy. VẬT LIỆU VÀ PHƯƠNG PHÁP Năm mươi răng tiền hàm dưới một chân răng đã được cắt bỏ từ mối nối men-xi măng và dụng cụ bằng hệ thống giũa quay ProTaper universal cùng với việc tưới rửa nhiều bằng natri hypoclorit 1,0% và nước cất. Trên cơ sở loại chất tạo phức được sử dụng để tưới rửa, các mẫu (n = 10) sau đó được phân ngẫu nhiên thành năm nhóm là: Nhóm I- axit etidronic 9%, Nhóm II- axit etidronic 18%, Nhóm III- SmearClear, Nhóm IV- MTAD và Nhóm V- nước muối sinh lý. Sau khi tưới rửa, tất cả các mẫu đều được rửa sạch, sấy khô và cắt theo chiều dọc để đánh giá việc loại bỏ lớp bôi trơn dưới kính hiển vi điện tử quét (2000X). Dữ liệu được phân tích thống kê bằng phân tích phương sai hai chiều và kiểm định Tukey sau thử nghiệm với độ tin cậy thống kê được đặt ở p < 0,5. KẾT QUẢ Kết quả cho thấy SmearClear là chất hiệu quả nhất trong việc loại bỏ lớp bôi trơn. Tuy nhiên, axit etidronic được phát hiện kém hiệu quả hơn cả SmearClear và MTAD. KẾT LUẬN Chất tạo phức rất cần thiết để loại bỏ hoàn toàn lớp bôi trơn kết hợp với dung môi hữu cơ."} +{"idx": 2000044, "en": "STATEMENT OF THE PROBLEM Use of traditional medicine to relieve human sufferings has a very long history. The effects of these plants in wound curing and subsequently making the best mucosa patch for treatment of oral ulcers is still under investigation. PURPOSE The main goal of present research work is to assess the efficacy of Myrtus communis L., Camellia sinensis L. and Zataria multiflora Boiss. on oral ulcer recovery process in rats. MATERIALS AND METHOD In this study, 60 healthy adult male rats in 5 groups were investigated. A wound with 2 mm diameter was punched into the hard palate of each rat. For topical application, a mucosa patch of materials or blank was packed into the wound. Histological samples were harvested on post injury days 2, 4, 6, and 8. RESULTS This study showed that there were no significant differences between groups in the reduction of weight. Comparison of clinical wound size showed that group Myrtus communis L had the greatest reduction in wound size on days 4, 6 and 8, which was significantly different from the other groups. The highest thickness of epithelium was observed in groups Myrtus communis L and Camellia sinensis L on days 6 and 8. Group Myrtus communis L showed the highest values on days 6 and 8. This study showed a lower mononuclear cell counts in group Myrtus communis L on days 6 and 8 compared to other groups which was statistically significant. CONCLUSION The results of the present study showed that Myrtus communis L. has significant effects on oral wound healing processes. These favorable results might introduce a new group of material or medicine derived from this plant.", "gemini": "TÓM TẮT VẤN ĐỀ Sử dụng y học cổ truyền để giảm đau cho con người đã có lịch sử rất lâu đời. Tác dụng của các loại cây này trong việc chữa lành vết thương và sau đó tạo ra miếng dán niêm mạc tốt nhất để điều trị loét miệng vẫn đang được nghiên cứu. MỤC ĐÍCH Mục tiêu chính của công trình nghiên cứu hiện tại là đánh giá hiệu quả của Myrtus communis L., Camellia sinensis L. và Zataria multiflora Boiss. về quá trình phục hồi loét miệng ở chuột. VẬT LIỆU VÀ PHƯƠNG PHÁP Trong nghiên cứu này, 60 con chuột đực trưởng thành khỏe mạnh ở 5 nhóm đã được nghiên cứu. Một vết thương có đường kính 2 mm được đục vào vòm miệng cứng của mỗi con chuột. Để bôi tại chỗ, một miếng dán niêm mạc làm từ vật liệu hoặc miếng dán trắng được nhét vào vết thương. Các mẫu mô học được thu thập vào các ngày sau chấn thương 2, 4, 6 và 8. KẾT QUẢ Nghiên cứu này cho thấy không có sự khác biệt đáng kể giữa các nhóm về việc giảm cân nặng. So sánh kích thước vết thương lâm sàng cho thấy nhóm Myrtus communis L có mức giảm kích thước vết thương lớn nhất vào các ngày 4, 6 và 8, có sự khác biệt đáng kể so với các nhóm khác. Độ dày biểu mô cao nhất được quan sát thấy ở các nhóm Myrtus communis L và Camellia sinensis L vào các ngày 6 và 8. Nhóm Myrtus communis L cho thấy các giá trị cao nhất vào các ngày 6 và 8. Nghiên cứu này cho thấy số lượng tế bào đơn nhân thấp hơn ở nhóm Myrtus communis L vào các ngày 6 và 8 so với các nhóm khác có ý nghĩa thống kê. KẾT LUẬN Kết quả của nghiên cứu hiện tại cho thấy Myrtus communis L. có tác dụng đáng kể đến quá trình lành vết thương ở miệng. Những kết quả thuận lợi này có thể giới thiệu một nhóm vật liệu hoặc thuốc mới có nguồn gốc từ loại cây này."} +{"idx": 2000045, "en": "STATEMENT OF THE PROBLEM Evaluation of the color stability of materials used for the fabrication of the base of complete and partial removable dentures provides important information in relation to their clinical service. The color stability of nylon-based polymers as denture bases, compared to that of acrylic resins, has not been extensively evaluated since to date. PURPOSE The aim of the present in vitro study was to evaluate the color stability of conventional polymethyl methacrylate and nylon-based polymer materials used for the fabrication of the base of complete and partial removable dentures. MATERIALS AND METHOD Twenty samples were evaluated in the present in vitro study. One nylon-based polymer and one heat-cured acrylic resin were seleted for the purpose of the study. Ten disk samples, measuring 10 mm in diameter and 2 mm in thickness, were prepared from each material according to the manufacturers' instructions. The samples were evaluted with the CIELAB sysem using spectrophtometery at baseline and after irradiation with UV light for 100 and 200 hours and thermocycling. One-way ANOVA and post hoc paired t-test were used to compare the color stability of the two groups. Statistical significance was set at p< 0.05. RESULTS After 100 hours of irradiation with UV light and thermocycling, color changes were tangible in both materials. After 200 hours of irradiation with UV light and thermocycling, both materials exhibited a decrease in glaze. In general, chromatic changes in the acrylic resin were more noticeable. CONCLUSION Accelerated aging affected the color stability of the acrylic resin and nylon-based polymer, p< 0.05 for Δa200 and Δb200 and ΔL100; however, at the end of the study both materials exhibited clinically acceptable color stability (ΔE<3.7).", "gemini": "TÓM TẮT VẤN ĐỀ Đánh giá độ bền màu của vật liệu được sử dụng để chế tạo nền của răng giả tháo lắp hoàn toàn và bán phần cung cấp thông tin quan trọng liên quan đến dịch vụ lâm sàng của chúng. Độ bền màu của vật liệu polyme nền nylon làm răng giả so với vật liệu nhựa acrylic vẫn chưa được đánh giá rộng rãi cho đến nay. MỤC ĐÍCH Mục đích của nghiên cứu trong ống nghiệm hiện tại là đánh giá độ bền màu của polyme methacrylate polymetyl thông thường và polyme nền nylon được sử dụng để chế tạo nền của răng giả tháo lắp hoàn toàn và bán phần. VẬT LIỆU VÀ PHƯƠNG PHÁP Hai mươi mẫu vật đã được đánh giá trong nghiên cứu trong ống nghiệm hiện tại. Một loại polyme nền nylon và một loại nhựa acrylic đóng rắn bằng nhiệt đã được lựa chọn cho mục đích của nghiên cứu. Mười mẫu đĩa có đường kính 10 mm và độ dày 2 mm được chế tạo từ mỗi vật liệu theo hướng dẫn của nhà sản xuất. Các mẫu vật được đánh giá bằng hệ thống CIELAB sử dụng phương pháp đo quang phổ ở thời điểm ban đầu và sau khi chiếu sáng bằng tia UV trong 100 và 200 giờ và chu kỳ nhiệt. Phân tích phương sai một chiều và kiểm định t ghép cặp sau khi kiểm định được sử dụng để so sánh độ bền màu của hai nhóm. Độ tin cậy thống kê được đặt ở mức p < 0,05. KẾT QUẢ Sau 100 giờ chiếu sáng bằng tia UV và chu kỳ nhiệt, sự thay đổi màu sắc đã được nhìn thấy rõ ở cả hai vật liệu. Sau 200 giờ chiếu sáng bằng tia UV và chu kỳ nhiệt, cả hai vật liệu đều cho thấy độ bóng giảm. Nhìn chung, sự thay đổi sắc độ của nhựa acrylic dễ nhận thấy hơn. KẾT LUẬN Sự lão hóa nhanh chóng ảnh hưởng đến độ bền màu của nhựa acrylic và polyme nền nylon, p < 0,05 đối với Δa200 và Δb200 và ΔL100; tuy nhiên, vào cuối nghiên cứu, cả hai vật liệu đều cho thấy độ bền màu có thể chấp nhận được về mặt lâm sàng (ΔE < 3,7)."} +{"idx": 2000046, "en": "STATEMENT OF THE PROBLEM Sinonasal papilloma (SNP) is a rare benign lesion characterized by high recurrence rate and malignant transformation. PURPOSE This study aimed to investigate the prevalence of human papilloma virus (HPV) infection in these lesions in South of Iran. MATERIALS AND METHOD In this cross sectional retrospective study, a total of 41 patients, 38 SNP and 3 SNP/Squamous cell carcinoma cases, from 2007 to 2014 were studied. Human papilloma virus (HPV) DNA detection was performed by nested PCR method and positive cases were analyzed for high risk HPV-16 and HPV-18. RESULTS HPV was detected in 31.7%; HPV- 16 in 4.9% and HPV 18 was not detected at all. Dysplastic epithelium was detected in 53% that was not associated with HPV. Three cases were accompanied with malignant transformation that HPV genome was detected in only one case and none of them were positive for HPV16 /18 genomic DNA. CONCLUSION Current research suggests that HPV may be involved in the development of SNP. But the high risk HPV is not important in malignant transformation. More studies are needed to elucidate the possible etiologic mechanism between HPV, inverted papilloma, and squamous cell carcinoma.", "gemini": "TÓM TẮT VẤN ĐỀ U nhú mũi xoang (SNP) là một tổn thương lành tính hiếm gặp có đặc điểm là tỷ lệ tái phát cao và chuyển dạng thành ác tính. MỤC ĐÍCH Nghiên cứu này nhằm mục đích điều tra tỷ lệ nhiễm virus papilloma ở người (HPV) ở những tổn thương này ở miền Nam Iran. VẬT LIỆU VÀ PHƯƠNG PHÁP Trong nghiên cứu hồi cứu cắt ngang này, tổng cộng 41 bệnh nhân, 38 trường hợp SNP và 3 trường hợp SNP/ung thư biểu mô tế bào vảy, từ năm 2007 đến năm 2014 đã được nghiên cứu. Phát hiện DNA của virus papilloma ở người (HPV) được thực hiện bằng phương pháp PCR lồng nhau và các trường hợp dương tính được phân tích về HPV-16 và HPV-18 có nguy cơ cao. KẾT QUẢ HPV được phát hiện ở 31,7%; HPV-16 ở 4,9% và HPV 18 không được phát hiện ở tất cả. Biểu mô loạn sản được phát hiện ở 53% không liên quan đến HPV. Ba trường hợp đi kèm với chuyển dạng ác tính mà bộ gen HPV chỉ được phát hiện ở một trường hợp và không trường hợp nào trong số chúng dương tính với DNA của bộ gen HPV16 / 18. KẾT LUẬN Nghiên cứu hiện tại cho thấy rằng HPV có thể liên quan đến sự phát triển của SNP. Nhưng HPV nguy cơ cao không quan trọng trong quá trình chuyển dạng ác tính. Cần nhiều nghiên cứu hơn để làm sáng tỏ cơ chế nguyên nhân có thể xảy ra giữa HPV, u nhú đảo ngược và ung thư biểu mô tế bào vảy."} +{"idx": 2000047, "en": "Solitary fibrous tumor has a wide spectrum of histopathologic features and many tumors show similar microscopic features. This similarity poses diagnostic challenges to the pathologists and immunohistochemical analysis is required in many cases. Moreover, it is a rare entity in orofacial region which consequently would make its diagnosis more challenging in oral cavity. The knowledge of various microscopic patterns of this tumor contributes to a proper diagnosis and prevents unnecessary treatment. This study reports a case of solitary fibrous tumor in the retromolar pad area and discusses its various histological features and differential diagnoses.", "gemini": "U sợi đơn độc có nhiều đặc điểm mô bệnh học và nhiều khối u có các đặc điểm vi mô tương tự nhau. Sự tương đồng này gây ra những thách thức về chẩn đoán đối với các nhà bệnh học và trong nhiều trường hợp cần phải phân tích miễn dịch mô. Hơn nữa, đây là một thực thể hiếm gặp ở vùng mặt miệng, do đó sẽ khiến việc chẩn đoán trở nên khó khăn hơn trong khoang miệng. Kiến thức về các mô hình vi mô khác nhau của khối u này góp phần vào chẩn đoán đúng và ngăn ngừa điều trị không cần thiết. Nghiên cứu này báo cáo một trường hợp u sợi đơn độc ở vùng đệm sau răng và thảo luận về các đặc điểm mô học và chẩn đoán phân biệt khác nhau của nó."} +{"idx": 2000048, "en": "Taurodontism is a relatively rare variance with a very low incidence in primary dentition and only a few cases have been reported in the literature. It stands a challenge when these teeth require pulp therapy. This case report illustrates the oral rehabilitation of multiple primary molars with taurodontism involving both the maxillary and mandibular arches, which is a rare occurrence.", "gemini": "Taurodontism là một biến thể tương đối hiếm với tỷ lệ mắc rất thấp ở răng sữa và chỉ có một số ít trường hợp được báo cáo trong các tài liệu. Nó trở thành một thách thức khi những chiếc răng này cần điều trị tủy. Báo cáo trường hợp này minh họa phục hồi răng miệng của nhiều răng hàm sữa bị taurodontism liên quan đến cả cung hàm trên và cung hàm dưới, đây là một trường hợp hiếm gặp."} +{"idx": 2000049, "en": "BACKGROUND In parallel with the obesity epidemic, liver transplantation for nonalcoholic steatohepatitis (NASH) is increasing dramatically in North America. Although survival outcomes are similar to other etiologies, liver transplantation in the NASH population has been associated with significantly increased resource utilization. We sought to compare outcomes between live donor liver transplantation (LDLT) and deceased donor liver transplantation (DDLT) at a high volume North American transplant center, with a particular focus on resource utilization. METHODS The study population consists of primary liver transplants performed for NASH at Toronto General Hospital from 2000 to 2014. Recipient characteristics, perioperative outcomes, graft and patient survivals, and resource utilization were compared for LDLT versus DDLT. RESULTS A total of 176 patients were included in the study (48 LDLT vs 128 DDLT). LDLT recipients had a lower model for end-stage liver disease score and were less frequently hospitalized prior to transplant. Estimated blood loss and early markers of graft injury were lower for LDLT. LDLT recipients had a significantly shorter hospitalization (intensive care unit, postoperative, and total hospitalization). CONCLUSIONS LDLT for NASH facilitates transplantation of patients at a less severe stage of disease, which appears to promote a faster postoperative recovery with less resource utilization.", "gemini": "BỐI CẢNH Song song với đại dịch béo phì, ghép gan do viêm gan nhiễm mỡ không do rượu (NASH) đang gia tăng đáng kể ở Bắc Mỹ. Mặc dù kết quả sống sót tương tự như các nguyên nhân khác, nhưng việc ghép gan ở những người mắc NASH có liên quan đến việc sử dụng nhiều nguồn lực hơn đáng kể. Chúng tôi tìm cách so sánh kết quả giữa ghép gan từ người hiến tặng sống (LDLT) và ghép gan từ người hiến tặng đã chết (DDLT) tại một trung tâm ghép tạng lớn ở Bắc Mỹ, với trọng tâm đặc biệt là việc sử dụng nguồn lực. PHƯƠNG PHÁP Dân số nghiên cứu bao gồm những ca ghép gan chính được thực hiện cho NASH tại Bệnh viện Đa khoa Toronto từ năm 2000 đến 2014. Các đặc điểm của người nhận, kết quả trong thời gian phẫu thuật, ghép và khả năng sống sót của bệnh nhân và việc sử dụng nguồn lực đã được so sánh cho LDLT so với DDLT. KẾT QUẢ Tổng cộng có 176 bệnh nhân được đưa vào nghiên cứu (48 LDLT so với 128 DDLT). Những người nhận LDLT có điểm mô hình bệnh gan giai đoạn cuối thấp hơn và ít thường xuyên phải nhập viện trước khi ghép. Lượng máu mất ước tính và các dấu hiệu tổn thương ghép sớm thấp hơn đối với LDLT. Những người nhận LDLT có thời gian nằm viện đáng kể ngắn hơn (đơn vị chăm sóc đặc biệt, hậu phẫu và tổng thời gian nằm viện). KẾT LUẬN LDLT cho NASH tạo điều kiện thuận lợi cho việc ghép bệnh nhân ở giai đoạn bệnh ít nghiêm trọng hơn, điều này dường như thúc đẩy quá trình phục hồi sau phẫu thuật nhanh hơn với việc sử dụng ít nguồn lực hơn."} +{"idx": 2000050, "en": "BACKGROUND Activins A and B, members of the TGF-β superfamily, are produced as part of the physiological response to tissue damage and the resulting proinflammatory response. Given that lung allograft reperfusion results in an inflammatory response, it is likely that the activins and their binding protein follistatin will form part of the regulatory response. There is a need to document the response of these proteins to allograft reperfusion to determine if there is a role for the use of follistatin to control the biological actions of the activins because some of these are potentially damaging. METHODS Serum from 48 consecutive patients undergoing lung transplantation (LTx) was collected at 2, 6, 12, and 26 weeks post-LTx. The serum levels of activin A and B and follistatin were measured by enzyme-linked immunosorbent assay and specific radioimmunoassays and compared with clinical events. RESULTS Serum activin A and B levels were at the upper limit of the normal ranges at 2 weeks post-LTx decreasing thereafter to 12 weeks post-LTx (P < 0.05). In contrast, serum follistatin levels were unchanged between 2 and 12 weeks, with a late significant increase at 24 week post-LTx (P < 0.01). Patients with primary graft dysfunction had lower serum follistatin levels (7.7 vs 9.5 ng/mL; P = 0.04) and a higher activin A/follistatin ratio (13.1 vs 10.4; P = 0.02) at 2 weeks post-LTx. CONCLUSIONS Activin and follistatin levels vary with time form LTX and reflect a proinflammatory environment. Future studies will elucidate associations with chronic lung allograft dysfunction and the therapeutic potential of exogenous follistatin administration.", "gemini": "BỐI CẢNH Activin A và B, các thành viên của siêu họ TGF-β, được sản xuất như một phần của phản ứng sinh lý đối với tổn thương mô và phản ứng viêm sau đó. Do tưới máu lại ghép phổi dẫn đến phản ứng viêm, nên có khả năng các activin và protein liên kết follistatin của chúng sẽ tạo thành một phần của phản ứng điều hòa. Cần phải ghi lại phản ứng của các protein này đối với tưới máu lại ghép để xác định xem có vai trò nào của việc sử dụng follistatin để kiểm soát các hoạt động sinh học của activin hay không vì một số trong số này có khả năng gây hại. PHƯƠNG PHÁP Huyết thanh từ 48 bệnh nhân liên tiếp được ghép phổi (LTx) được thu thập vào tuần thứ 2, 6, 12 và 26 sau LTx. Nồng độ activin A và B và follistatin trong huyết thanh được đo bằng xét nghiệm miễn dịch hấp thụ liên kết với enzyme và xét nghiệm miễn dịch phóng xạ đặc hiệu và so sánh với các biến cố lâm sàng. KẾT QUẢ Nồng độ activin A và B trong huyết thanh ở mức giới hạn trên của phạm vi bình thường sau 2 tuần sau LTx, sau đó giảm xuống còn 12 tuần sau LTx (P < 0,05). Ngược lại, nồng độ follistatin trong huyết thanh không thay đổi giữa tuần thứ 2 và tuần thứ 12, với sự gia tăng đáng kể muộn ở tuần thứ 24 sau LTx (P < 0,01). Bệnh nhân bị rối loạn chức năng ghép chính có nồng độ follistatin trong huyết thanh thấp hơn (7,7 so với 9,5 ng/mL; P = 0,04) và tỷ lệ activin A/follistatin cao hơn (13,1 so với 10,4; P = 0,02) sau 2 tuần sau LTx. KẾT LUẬN Nồng độ activin và follistatin thay đổi theo thời gian sau LTX và phản ánh một môi trường viêm. Các nghiên cứu trong tương lai sẽ làm sáng tỏ mối liên quan với rối loạn chức năng ghép phổi mạn tính và tiềm năng điều trị của việc dùng follistatin ngoại sinh."} +{"idx": 2000051, "en": "BACKGROUND The interplay between viral infection and alloimmunity is known to influence the fate of transplanted organs. Clarifying how local virus-associated inflammation/injury and antiviral immunity can alter host alloimmune responses in transplantation remains a critical question. METHODS We used a mouse model of polyomavirus (PyV) infection and kidney transplantation to investigate the roles of direct viral pathology, the antiviral immune response, and alloimmunity in the pathogenesis of PyV-associated allograft injury. We have previously shown that an effective primary T cell response is required in PyV-associated graft injury. RESULTS Here we show that the transfer of primed antidonor, but not antiviral, T cells results in PyV-associated allograft injury. In further studies, we use a surrogate minor antigen model (ovalbumin) and show that only antidonor specific T cells and not antiviral specific T cells are sufficient to mediate injury. Lastly, we demonstrate that local but not systemic virus-mediated inflammation and injury within the graft itself are required. CONCLUSIONS These data suggest that in this mouse model, the predominant mechanism of allograft injury in PyV-associated injury is due to an augmented alloimmune T cell response driven by virus-induced inflammation/injury within the graft. These studies highlight the important interplay between viral infection and alloimmunity in a model system.", "gemini": "BỐI CẢNH Sự tương tác giữa nhiễm virus và miễn dịch dị ứng được biết là ảnh hưởng đến số phận của các cơ quan được ghép. Việc làm sáng tỏ cách viêm/tổn thương liên quan đến virus tại chỗ và miễn dịch chống vi-rút có thể làm thay đổi đáp ứng miễn dịch dị ứng của vật chủ trong ghép vẫn là một câu hỏi quan trọng. PHƯƠNG PHÁP Chúng tôi đã sử dụng một mô hình chuột nhiễm polyomavirus (PyV) và ghép thận để nghiên cứu vai trò của bệnh lý do virus trực tiếp, đáp ứng miễn dịch chống vi-rút và miễn dịch dị ứng trong sinh bệnh học của tổn thương ghép liên quan đến PyV. Trước đây, chúng tôi đã chỉ ra rằng cần có đáp ứng tế bào T sơ cấp hiệu quả trong tổn thương ghép liên quan đến PyV. KẾT QUẢ Ở đây chúng tôi chỉ ra rằng việc truyền tế bào T chống lại người hiến tặng đã được mồi, nhưng không phải tế bào T chống vi-rút, dẫn đến tổn thương ghép liên quan đến PyV. Trong các nghiên cứu sâu hơn, chúng tôi sử dụng một mô hình kháng nguyên phụ thay thế (ovalbumin) và chỉ ra rằng chỉ có tế bào T đặc hiệu chống lại người hiến tặng chứ không phải tế bào T đặc hiệu chống vi-rút mới đủ để gây ra tổn thương. Cuối cùng, chúng tôi chứng minh rằng viêm và tổn thương do virus gây ra tại chỗ nhưng không phải toàn thân trong chính bản ghép là cần thiết. KẾT LUẬN Dữ liệu này cho thấy rằng trong mô hình chuột này, cơ chế tổn thương ghép chiếm ưu thế trong tổn thương liên quan đến PyV là do đáp ứng tế bào T miễn dịch dị ứng tăng lên do viêm/tổn thương do virus gây ra trong ghép. Các nghiên cứu này làm nổi bật sự tương tác quan trọng giữa nhiễm virus và miễn dịch dị ứng trong một hệ thống mô hình."} +{"idx": 2000052, "en": "BACKGROUND Cardiovascular disease (CVD) is a major cause of mortality among kidney transplant recipients (KTRs). These patients have a high prevalence of risk factors, such as hypertension, diabetes, and dyslipidemia. Despite regular medical care, few of them reach the recommended therapeutic targets. The objective of this study is to describe KTRs' perspectives on CVD and related risk factors, as well as their priorities for posttransplant care. METHODS Twenty-six KTRs participated in a semistructured interview about their personal experience and offered their perspectives on CVD risk factors posttransplant. The interview was digitally recorded and the transcripts were analyzed using a thematic and content methodology. RESULTS CVD and related risk factors appear to be underestimated and trivialized. Only 2 of 26 patients identified CVD prevention and treatment as a priority. The most important posttransplant priorities identified by patients were related to immunosuppressive drugs (13 of 26), posttransplant follow-up (10) and graft survival (9). However, 21 of 26 patients stated they wanted to be better informed about posttransplant CVD risk factors. CONCLUSIONS CVD and related risk factors are not a priority for KTRs, and the importance of CVD is underestimated and trivialized. KTRs did recommend that tailored information be provided by various professionals and at several points in the transplantation process. This knowledge will help us develop a new approach to increase awareness of posttransplant CVD and related risk factors.", "gemini": "BỐI CẢNH Bệnh tim mạch (CVD) là nguyên nhân chính gây tử vong ở những người nhận ghép thận (KTR). Những bệnh nhân này có tỷ lệ mắc các yếu tố nguy cơ cao, chẳng hạn như tăng huyết áp, tiểu đường và rối loạn lipid máu. Mặc dù được chăm sóc y tế thường xuyên, nhưng rất ít người đạt được mục tiêu điều trị được khuyến cáo. Mục tiêu của nghiên cứu này là mô tả quan điểm của KTR về CVD và các yếu tố nguy cơ liên quan, cũng như các ưu tiên của họ đối với việc chăm sóc sau ghép. PHƯƠNG PHÁP Hai mươi sáu KTR đã tham gia trả lời phỏng vấn bán cấu trúc về kinh nghiệm cá nhân của họ và đưa ra quan điểm của họ về các yếu tố nguy cơ CVD sau ghép. Cuộc phỏng vấn đã được ghi âm kỹ thuật số và các bản ghi được phân tích bằng phương pháp nội dung và chủ đề. KẾT QUẢ CVD và các yếu tố nguy cơ liên quan dường như bị đánh giá thấp và coi nhẹ. Chỉ có 2 trong số 26 bệnh nhân xác định phòng ngừa và điều trị CVD là ưu tiên hàng đầu. Các ưu tiên quan trọng nhất sau ghép mà bệnh nhân xác định có liên quan đến thuốc ức chế miễn dịch (13 trong số 26), theo dõi sau ghép (10) và sự sống còn của ghép (9). Tuy nhiên, 21 trong số 26 bệnh nhân cho biết họ muốn được cung cấp thông tin tốt hơn về các yếu tố nguy cơ CVD sau ghép. KẾT LUẬN CVD và các yếu tố nguy cơ liên quan không phải là ưu tiên đối với KTR, và tầm quan trọng của CVD bị đánh giá thấp và coi nhẹ. KTR đã khuyến nghị rằng thông tin phù hợp nên được cung cấp bởi nhiều chuyên gia khác nhau và tại một số thời điểm trong quá trình ghép. Kiến thức này sẽ giúp chúng tôi phát triển một phương pháp mới để nâng cao nhận thức về CVD sau ghép và các yếu tố nguy cơ liên quan."} +{"idx": 2000053, "en": "BACKGROUND Prostaglandins are often administered after liver transplantation (LT) to diminish ischemia-reperfusion injury (IRI), to favor liver recovery and to prevent vascular thrombosis. Possible beneficial effects in adult liver recipients are controversial, but the single existing pediatric small case series shows no significant impact of prostaglandin administration after LT. The purpose of this study was to analyze the effect of the prostaglandin dinoprostone in pediatric liver recipients. METHODS A retrospective analysis of 41 children (<16 years) who underwent LT between March 2008 and December 2013 was performed. Dinoprostone was administered at a rate from 0.1 to a maximum of 0.6 μg/kg per hour immediately after LT and for a maximum of 5 days. Effect of dinoprostone on post-LT IRI and hepatic function up to 60 postoperative days and number of hypotensive episodes were analyzed. RESULTS The median cumulative dose of dinoprostone was 28 μg/kg (interquartile range, 23.2). Dinoprostone had no significant effect on post-LT liver function tests and factor V levels at any of the administered dosages. There was no significant association between the total quantity of vasopressor given and the number of hypotensive episodes observed in 8 patients. One patient showed a short-lasting hypotension, possibly related to the administration of dinoprostone. CONCLUSIONS This study did not show, at any dosage between 0.1 and 0.6 μg/kg per hour, any differences in beneficial or harmful effects of high- or low-dose dinoprostone administered immediately after pediatric LT on markers of IRI, hepatic function, or hypotension.", "gemini": "BỐI CẢNH Prostaglandin thường được dùng sau ghép gan (LT) để làm giảm tổn thương thiếu máu cục bộ tái tưới máu (IRI), thúc đẩy gan phục hồi và ngăn ngừa huyết khối mạch. Các tác dụng có lợi có thể ở người lớn nhận gan vẫn còn gây tranh cãi, nhưng loạt ca bệnh nhỏ ở nhi khoa hiện có duy nhất không cho thấy tác động đáng kể của việc dùng prostaglandin sau LT. Mục đích của nghiên cứu này là phân tích tác dụng của prostaglandin dinoprostone ở những người nhận gan nhi khoa. PHƯƠNG PHÁP Đã tiến hành phân tích hồi cứu đối với 41 trẻ em (<16 tuổi) đã ghép gan trong thời gian từ tháng 3 năm 2008 đến tháng 12 năm 2013. Dinoprostone được dùng với liều từ 0,1 đến tối đa 0,6 μg/kg mỗi giờ ngay sau LT và trong tối đa 5 ngày. Tác dụng của dinoprostone đối với IRI sau LT và chức năng gan lên đến 60 ngày sau phẫu thuật và số đợt hạ huyết áp đã được phân tích. KẾT QUẢ Liều tích lũy trung bình của dinoprostone là 28 μg/kg (khoảng cách giữa các tứ phân vị, 23,2). Dinoprostone không có tác dụng đáng kể đối với các xét nghiệm chức năng gan sau LT và mức độ yếu tố V ở bất kỳ liều dùng nào. Không có mối liên hệ đáng kể nào giữa tổng lượng thuốc vận mạch được dùng và số đợt hạ huyết áp được quan sát thấy ở 8 bệnh nhân. Một bệnh nhân bị hạ huyết áp trong thời gian ngắn, có thể liên quan đến việc dùng dinoprostone. KẾT LUẬN Nghiên cứu này không chỉ ra, ở bất kỳ liều dùng nào từ 0,1 đến 0,6 μg/kg mỗi giờ, bất kỳ sự khác biệt nào về tác dụng có lợi hoặc có hại của dinoprostone liều cao hoặc liều thấp được dùng ngay sau LT nhi khoa đối với các dấu hiệu của IRI, chức năng gan hoặc hạ huyết áp."} +{"idx": 2000054, "en": "BACKGROUND Hepatoblastoma is the most common primary malignant liver tumor in children and is usually diagnosed during the first 3 years of life. Overall survival has increased 50% due to chemotherapeutic schemes, expertise surgery centers, and liver transplantation. METHODS A retrospective collection of data was performed from pediatric patients with diagnosis of hepatoblastoma. Variables included demographic, diagnostic tools and histological classification; chemotherapy and surgical treatment; and outcomes and patient survival. The PRETEXT classification was applied, which included the risk evaluation, and according to the medical criterion in an individualized way, underwent resection or transplant. The morbidity of patients was evaluated by the Clavien-Dindo classification. Statistical analysis was performed according to the distribution of data and the survival analysis was carried out using the Kaplan-Meier method. RESULTS The patients (n = 16) were divided in a resection group (n = 8) and a transplant group (n = 8). The median age at the time of diagnosis was 13.5 months. The motive for the initial consultation was the discovery of a mass; all patients had high levels of α-fetoprotein and an imaging study. Ten of 16 patients required chemotherapy before the surgical procedure. In the resection group, 5 of 8 patients were classified as Clavien I and 4 of 8 patients of the transplant group were classified as Clavien II. Patient survival at 30 months was 100% in the resection group and 65% in the liver transplantation group. CONCLUSIONS To our knowledge, this is the first case report of pediatric patients with hepatoblastoma and liver resection or transplant in Colombia and Latin America. Our results are comparable with the series worldwide, showing that resection and transplant increase the survival of the pediatric patients with hepatoblastoma. It is important to advocate for an increase of reporting in the scientific literature in Latin America.", "gemini": "BỐI CẢNH U gan nguyên bào là khối u gan ác tính nguyên phát phổ biến nhất ở trẻ em và thường được chẩn đoán trong 3 năm đầu đời. Tỷ lệ sống sót chung đã tăng 50% nhờ các phác đồ hóa trị liệu, chuyên môn phẫu thuật và ghép gan. PHƯƠNG PHÁP Đã tiến hành thu thập dữ liệu hồi cứu từ những bệnh nhi được chẩn đoán mắc u gan nguyên bào. Các biến bao gồm thông tin nhân khẩu học, công cụ chẩn đoán và phân loại mô học; hóa trị liệu và điều trị phẫu thuật; và kết quả cũng như khả năng sống sót của bệnh nhân. Phân loại PRETEXT đã được áp dụng, bao gồm đánh giá rủi ro và theo tiêu chuẩn y khoa theo cách cá nhân hóa, đã được cắt bỏ hoặc ghép. Tỷ lệ mắc bệnh của bệnh nhân được đánh giá theo phân loại Clavien-Dindo. Phân tích thống kê được thực hiện theo phân bố dữ liệu và phân tích khả năng sống sót được thực hiện bằng phương pháp Kaplan-Meier. KẾT QUẢ Bệnh nhân (n = 16) được chia thành nhóm cắt bỏ (n = 8) và nhóm ghép (n = 8). Độ tuổi trung bình tại thời điểm chẩn đoán là 13,5 tháng. Lý do khám ban đầu là phát hiện khối u; tất cả bệnh nhân đều có nồng độ alpha-fetoprotein cao và một nghiên cứu hình ảnh. Mười trong số 16 bệnh nhân cần hóa trị trước khi phẫu thuật. Trong nhóm cắt bỏ, 5/8 bệnh nhân được phân loại là Clavien I và 4/8 bệnh nhân của nhóm ghép được phân loại là Clavien II. Tỷ lệ sống sót của bệnh nhân sau 30 tháng là 100% ở nhóm cắt bỏ và 65% ở nhóm ghép gan. KẾT LUẬN Theo hiểu biết của chúng tôi, đây là báo cáo trường hợp đầu tiên về bệnh nhi mắc u gan nguyên bào và cắt bỏ hoặc ghép gan ở Colombia và Châu Mỹ Latinh. Kết quả của chúng tôi tương đương với các loạt nghiên cứu trên toàn thế giới, cho thấy cắt bỏ và ghép làm tăng khả năng sống sót của bệnh nhi mắc u gan nguyên bào. Điều quan trọng là phải ủng hộ việc tăng cường báo cáo trên các tạp chí khoa học ở Châu Mỹ Latinh."} +{"idx": 2000055, "en": "BACKGROUND Previous analyses of the United States transplant database regarding long-term outcomes in kidney transplantation have shown minimal improvement in the rate of long-term graft loss. This study sought to analyze intermediate-term outcomes and graft function at 6 months in kidney transplantation in adult living and deceased donor recipients in the last decade. METHODS Survival analysis was performed based on the year of transplant between 6 months and 3 years' posttransplant. The Chronic Kidney Disease Epidemiology Collaboration estimated glomerular filtration rate (eGFR) was determined at 6 months. RESULTS The unadjusted graft survival between 6 months and 3 years improved significantly in the latter half of the decade in both deceased and living donor kidney recipients. Cox analysis showed a 33% reduction in the rate of graft loss and that the improvement in graft survival was due to similar improvements in both death-censored graft and death with graft function survival. A 10% improvement in median eGFR occurred despite worsening donor demographics over time in both donor types. This improvement in eGFR and graft survival occurred in association with a consolidation of chronic discharge immunosuppression from a variety of combinations to over 85% of recipients receiving tacrolimus and mycophenolate derivative immunosuppression. CONCLUSIONS In the latter half of last decade graft survival improved in adult kidney transplant recipients. The improvement in graft survival occurred in temporal association with an improvement in median eGFR at 6 months and consolidation of discharge immunosuppression in most patients to tacrolimus and mycophenolate derivatives.", "gemini": "BỐI CẢNH Các phân tích trước đây về cơ sở dữ liệu ghép tạng của Hoa Kỳ liên quan đến kết quả dài hạn trong ghép thận đã cho thấy sự cải thiện tối thiểu về tỷ lệ mất ghép dài hạn. Nghiên cứu này tìm cách phân tích kết quả trung gian và chức năng ghép sau 6 tháng ở những người nhận ghép thận từ người hiến tặng sống và đã chết ở người lớn trong thập kỷ qua. PHƯƠNG PHÁP Phân tích khả năng sống sót được thực hiện dựa trên năm ghép giữa 6 tháng và 3 năm sau ghép. Tỷ lệ lọc cầu thận ước tính của Tổ chức hợp tác dịch tễ học bệnh thận mãn tính (eGFR) được xác định sau 6 tháng. KẾT QUẢ Tỷ lệ sống sót của ghép chưa điều chỉnh giữa 6 tháng và 3 năm đã được cải thiện đáng kể trong nửa cuối thập kỷ ở cả những người nhận thận từ người hiến tặng đã chết và đang sống. Phân tích Cox cho thấy tỷ lệ mất ghép giảm 33% và sự cải thiện về khả năng sống sót của ghép là do những cải thiện tương tự ở cả ghép kiểm duyệt tử vong và tử vong với khả năng sống sót của ghép. eGFR trung bình cải thiện 10% mặc dù thông tin nhân khẩu học của người hiến tặng ngày càng xấu đi theo thời gian ở cả hai loại người hiến tặng. Sự cải thiện về eGFR và khả năng sống sót của ghép này xảy ra liên quan đến việc hợp nhất ức chế miễn dịch sau xuất viện mạn tính từ nhiều loại kết hợp đến hơn 85% người nhận ức chế miễn dịch tacrolimus và dẫn xuất mycophenolate. KẾT LUẬN Trong nửa cuối thập kỷ trước, khả năng sống sót của ghép đã được cải thiện ở những người nhận ghép thận ở người lớn. Sự cải thiện khả năng sống sót của ghép xảy ra liên quan về mặt thời gian với sự cải thiện eGFR trung bình sau 6 tháng và hợp nhất ức chế miễn dịch sau xuất viện ở hầu hết bệnh nhân thành dẫn xuất tacrolimus và mycophenolate."} +{"idx": 2000056, "en": "Previous research has indicated that the cyclic AMP (cAMP) signal transduction system plays an important role in the predisposition to and development of ethanol abuse in humans. Our laboratory has demonstrated that ethanol is capable of enhancing adenylyl cyclase (AC) activity. This effect is AC isoform-specific; type 7 AC (AC7) is most enhanced by ethanol. Therefore, we hypothesized that the expression of a specific AC isoform will play a role on the effect of ethanol on cAMP regulated gene expression. We employed NIH 3T3 cells transfected with AC7 or AC3 as a model system. To evaluate ethanol's effects on cAMP regulated gene expression, a luciferase reporter gene driven by a cAMP inducing artificial promoter was utilized. Stimulation of AC activity leads to an increase in the reporter gene activity. This increase was enhanced in the presence of ethanol in cells expressing AC7, while cells expressing AC3 did not respond to ethanol. cAMP reporter gene expression was increased in the presence of 8-bromo-cAMP; this expression was not enhanced by ethanol. These observations are consistent with our hypothesis. The basal level of CREB phosphorylation was high and did not change by cAMP stimulation or in the presence of ethanol. However, there were significant changes in the TORC3 amount in nuclei depending on stimulation conditions. The results suggest that nuclear translocation of TORC3 plays a more important role than CREB phosphorylation in the observed changes in the cAMP driven reporter gene activity.", "gemini": "Các nghiên cứu trước đây đã chỉ ra rằng hệ thống truyền tín hiệu cAMP (cAMP) đóng vai trò quan trọng trong khuynh hướng và sự phát triển của tình trạng lạm dụng rượu ở người. Phòng thí nghiệm của chúng tôi đã chứng minh rằng rượu có khả năng tăng cường hoạt động của adenylyl cyclase (AC). Hiệu ứng này là đặc hiệu đối với isoform AC; AC7 loại 7 được rượu tăng cường nhiều nhất. Do đó, chúng tôi đưa ra giả thuyết rằng sự biểu hiện của một isoform AC cụ thể sẽ đóng vai trò vào tác dụng của rượu đối với biểu hiện gen do cAMP điều hòa. Chúng tôi sử dụng các tế bào NIH 3T3 được chuyển gen với AC7 hoặc AC3 làm hệ thống mô hình. Để đánh giá tác dụng của rượu đối với biểu hiện gen do cAMP điều hòa, một gen báo cáo luciferase được điều khiển bởi một chất thúc đẩy nhân tạo gây ra cAMP đã được sử dụng. Kích thích hoạt động AC dẫn đến tăng hoạt động của gen báo cáo. Sự gia tăng này được tăng cường khi có sự hiện diện của rượu trong các tế bào biểu hiện AC7, trong khi các tế bào biểu hiện AC3 không phản ứng với rượu. Biểu hiện gen báo cáo cAMP tăng lên khi có 8-bromo-cAMP; biểu hiện này không được rượu tăng cường. Những quan sát này phù hợp với giả thuyết của chúng tôi. Mức cơ bản của quá trình phosphoryl hóa CREB là cao và không thay đổi khi kích thích cAMP hoặc khi có rượu. Tuy nhiên, có những thay đổi đáng kể về lượng TORC3 trong nhân tùy thuộc vào điều kiện kích thích. Kết quả cho thấy sự chuyển vị vào nhân của TORC3 đóng vai trò quan trọng hơn quá trình phosphoryl hóa CREB trong những thay đổi được quan sát thấy về hoạt động của gen báo cáo do cAMP thúc đẩy."} +{"idx": 2000057, "en": "T follicular helper (Tfh) CD4 cells are crucial providers of B cell help during adaptive immune responses. A circulating population of CD4 T cells, termed cTfh, have similarity to lymphoid Tfh, can provide B cell help, and responded to influenza vaccination. However, it is unclear whether human vaccination-induced cTfh respond in an antigen-specific manner and whether they form long-lasting memory. Here, we identified a cTfh population that expressed multiple T cell activation markers and could be readily identified by coexpression of ICOS and CD38. This subset expressed more Bcl-6, c-Maf, and IL-21 than other blood CD4 subsets. Influenza vaccination induced a strong response in the ICOS+CD38+ cTfh at day 7, and this population included hemagglutinin-specific cells by tetramer staining and antigen-stimulated Activation Induced Marker (AIM) expression. Moreover, TCRB sequencing identified a clonal response in ICOS+CD38+ cTfh that correlated strongly with the increased circulating ICOS+CD38+ cTfh frequency and the circulating plasmablast response. In subjects who received successive annual vaccinations, a recurrent oligoclonal response was identified in the ICOS+CD38+ cTfh subset at 7 days after every vaccination. These oligoclonal responses in ICOS+CD38+ cTfh after vaccination persisted in the ICOS-CD38- cTfh repertoire in subsequent years, suggesting clonal maintenance in a memory reservoir in the more-stable ICOS-CD38- cTfh subset. These data highlight the antigen-specificity, lineage relationships and memory properties of human cTfh responses to vaccination, providing new avenues for tracking and monitoring cTfh responses during infection and vaccination in humans.", "gemini": "Các tế bào T hỗ trợ nang T (Tfh) CD4 là những nhà cung cấp quan trọng về sự trợ giúp của tế bào B trong các phản ứng miễn dịch thích ứng. Một quần thể tế bào T CD4 lưu hành, được gọi là cTfh, có điểm tương đồng với Tfh bạch huyết, có thể cung cấp sự trợ giúp của tế bào B và phản ứng với vắc-xin cúm. Tuy nhiên, không rõ liệu cTfh do vắc-xin ở người có phản ứng theo cách đặc hiệu với kháng nguyên hay không và liệu chúng có tạo ra trí nhớ lâu dài hay không. Ở đây, chúng tôi xác định được một quần thể cTfh biểu hiện nhiều dấu hiệu hoạt hóa tế bào T và có thể dễ dàng nhận dạng bằng sự đồng biểu hiện của ICOS và CD38. Tập hợp con này biểu hiện nhiều Bcl-6, c-Maf và IL-21 hơn các tập hợp con CD4 trong máu khác. Vắc-xin cúm gây ra phản ứng mạnh ở cTfh ICOS+CD38+ vào ngày thứ 7 và quần thể này bao gồm các tế bào đặc hiệu với hemagglutinin bằng nhuộm tetrame và biểu hiện Dấu hiệu hoạt hóa do kháng nguyên kích thích (AIM). Hơn nữa, giải trình tự TCRB xác định được phản ứng vô tính ở cTfh ICOS+CD38+ có tương quan mạnh với tần số cTfh ICOS+CD38+ lưu hành tăng lên và phản ứng tế bào tương bào lưu hành. Ở những đối tượng được tiêm vắc-xin hàng năm liên tiếp, một phản ứng vô tính tái phát đã được xác định ở tập hợp con cTfh ICOS+CD38+ sau 7 ngày sau mỗi lần tiêm vắc-xin. Những phản ứng vô tính này ở cTfh ICOS+CD38+ sau khi tiêm vắc-xin vẫn tồn tại trong các tế bào cTfh ICOS-CD38- trong những năm tiếp theo, cho thấy sự duy trì vô tính trong một hồ chứa trí nhớ trong tập hợp con cTfh ICOS-CD38- ổn định hơn. Các dữ liệu này làm nổi bật tính đặc hiệu của kháng nguyên, mối quan hệ dòng dõi và các đặc tính trí nhớ của phản ứng cTfh của con người đối với vắc-xin, cung cấp những hướng đi mới để theo dõi và giám sát phản ứng cTfh trong quá trình nhiễm trùng và tiêm vắc-xin ở người."} +{"idx": 2000058, "en": "The study of how the human brain develops has always been a challenge and an interest to the scientific community. In recent years, new evidence has suggested that many neuropsychiatric disorders may originate from aberrations early in development. This discovery necessitates the application of methodologies that make possible the investigation of human brain development in vivo and across the lifespan. In this commentary, we present evidence that the advent of structural neuroimaging has specifically and significantly contributed critical information about the developmental trajectories of postnatal human brain development that would otherwise not have been possible. We believe that this is particularly relevant to present day research as it has become increasingly clear that growth trajectories within the brain might serve as an endophenotype for a number of factors, ranging from IQ to psychiatric illness. We highlight seminal early works that helped to jumpstart the field of developmental neuroimaging and which inspired incredible new advances in neuroimaging methodologies that are being developed and applied in the field today.", "gemini": "Việc nghiên cứu về cách phát triển của não người luôn là một thách thức và là mối quan tâm của cộng đồng khoa học. Trong những năm gần đây, bằng chứng mới đã chỉ ra rằng nhiều rối loạn tâm thần kinh có thể bắt nguồn từ những bất thường trong giai đoạn đầu phát triển. Khám phá này đòi hỏi phải áp dụng các phương pháp luận giúp có thể điều tra sự phát triển của não người trong cơ thể sống và trong suốt vòng đời. Trong bài bình luận này, chúng tôi trình bày bằng chứng cho thấy sự ra đời của kỹ thuật chụp ảnh thần kinh cấu trúc đã đóng góp cụ thể và đáng kể thông tin quan trọng về quỹ đạo phát triển của sự phát triển não ở người sau sinh mà nếu không thì sẽ không thể thực hiện được. Chúng tôi tin rằng điều này đặc biệt có liên quan đến nghiên cứu ngày nay vì ngày càng rõ ràng rằng các quỹ đạo tăng trưởng trong não có thể đóng vai trò là một kiểu hình cuối cho một số yếu tố, từ IQ đến bệnh tâm thần. Chúng tôi nêu bật những công trình ban đầu có ảnh hưởng giúp khởi động lĩnh vực chụp ảnh thần kinh phát triển và truyền cảm hứng cho những tiến bộ mới đáng kinh ngạc trong các phương pháp chụp ảnh thần kinh đang được phát triển và áp dụng trong lĩnh vực này ngày nay."} +{"idx": 2000059, "en": "Deep brain stimulation (DBS) has proven to be an effective treatment for neurologic disorders such as Parkinson's disease, and is currently being investigated as a therapy for psychiatric diseases such as addiction, major depressive disorder, and obsessive compulsive disorder. In this commentary, we review and discuss the findings presented in the Letter to the Editor entitled \"Attitudes towards treating addiction with deep brain stimulation,\" written by Ali et al1. The survey presented in this Letter reported general approval for examining the effects of DBS on addictive disorders in a clinical trial, but highlighted critical areas of concern including informed consent, patient autonomy, appropriate medical practice, passing of clinical trial milestones, and implications on law enforcement.", "gemini": "Kích thích não sâu (DBS) đã được chứng minh là một phương pháp điều trị hiệu quả cho các rối loạn thần kinh như bệnh Parkinson và hiện đang được nghiên cứu như một liệu pháp cho các bệnh tâm thần như nghiện ngập, rối loạn trầm cảm chính và rối loạn ám ảnh cưỡng chế. Trong bài bình luận này, chúng tôi xem xét và thảo luận về những phát hiện được trình bày trong Thư gửi Biên tập viên có tiêu đề \"Thái độ đối với việc điều trị nghiện bằng kích thích não sâu\", do Ali et al1 viết. Cuộc khảo sát được trình bày trong Thư này báo cáo sự chấp thuận chung đối với việc kiểm tra tác dụng của DBS đối với các rối loạn gây nghiện trong một thử nghiệm lâm sàng, nhưng nêu bật các lĩnh vực quan tâm quan trọng bao gồm sự đồng ý được thông báo, quyền tự chủ của bệnh nhân, thực hành y tế thích hợp, việc vượt qua các mốc thử nghiệm lâm sàng và tác động đến việc thực thi pháp luật."} +{"idx": 2000060, "en": "OBJECTIVES Lupus flares are triggered by environmental agents that cause oxidative stress, but the mechanisms involved are unclear. The flares are characterized by oxidative modifications of proteins by 4-hydroxynonenals, malondialdehydes, carbonyls and nitration. These modifications have been proposed to induce and perpetuate lupus flares by \"altered self\" mechanisms. An epigenetically altered CD4+CD28+ T cell subset, caused at least in part by nitration of T cell signaling molecules, is found in patients with active lupus, and nitrated T cells are sufficient to cause lupus-like autoimmunity in animal models. The relation of protein 4-hydroxynonenals, malondialdehydes, carbonyls and nitration to lupus flares though, is unknown. We tested if the size of the epigenetically altered subset is related to disease activity and one or more of these oxidative modifications in lupus patients. We also tested the relationship between subset size, disease activity and the same oxidative modifications in Sjogren's syndrome, another autoimmune disease also associated with oxidative stress and characterized by anti-nuclear antibodies and the presence of the subset. METHODS Lupus flare severity was quantitated using the Systemic Lupus Erythematosus Disease Activity Index, and Sjogren's flare severity using the European Sjogren's Syndrome Disease Activity Index. Subset size was determined by flow cytometry. Protein modifications were determined by ELISA. RESULTS Only protein nitration correlated with the size of the subset in lupus and Sjogren's syndrome. CONCLUSIONS These results support a role for protein nitration in subset size and lupus flare severity. Protein nitration may also contribute to autoantibody formation in Sjogren's syndrome.", "gemini": "MỤC TIÊU Các đợt bùng phát lupus được kích hoạt bởi các tác nhân môi trường gây ra stress oxy hóa, nhưng các cơ chế liên quan vẫn chưa rõ ràng. Các đợt bùng phát được đặc trưng bởi sự biến đổi oxy hóa của protein bởi 4-hydroxynonenals, malondialdehydes, carbonyls và nitrat hóa. Những biến đổi này đã được đề xuất để gây ra và duy trì các đợt bùng phát lupus bằng các cơ chế \"tự thay đổi\". Một phân nhóm tế bào T CD4+CD28+ bị thay đổi về mặt biểu sinh, ít nhất một phần là do nitrat hóa các phân tử truyền tín hiệu của tế bào T, được tìm thấy ở những bệnh nhân lupus đang hoạt động và các tế bào T bị nitrat hóa đủ để gây ra tình trạng tự miễn giống lupus ở các mô hình động vật. Tuy nhiên, mối quan hệ của protein 4-hydroxynonenals, malondialdehydes, carbonyls và nitrat hóa với các đợt bùng phát lupus vẫn chưa được biết rõ. Chúng tôi đã kiểm tra xem kích thước của phân nhóm bị thay đổi về mặt biểu sinh có liên quan đến hoạt động của bệnh và một hoặc nhiều hơn những biến đổi oxy hóa này ở bệnh nhân lupus hay không. Chúng tôi cũng đã kiểm tra mối quan hệ giữa kích thước phân nhóm, hoạt động của bệnh và các biến đổi oxy hóa tương tự ở hội chứng Sjogren, một bệnh tự miễn khác cũng liên quan đến stress oxy hóa và được đặc trưng bởi các kháng thể kháng nhân và sự hiện diện của phân nhóm. PHƯƠNG PHÁP Mức độ nghiêm trọng của đợt bùng phát lupus được định lượng bằng Chỉ số hoạt động bệnh lupus ban đỏ hệ thống và mức độ nghiêm trọng của đợt bùng phát Sjogren bằng Chỉ số hoạt động bệnh hội chứng Sjogren của Châu Âu. Kích thước phân nhóm được xác định bằng phương pháp đo lưu lượng tế bào. Các biến đổi protein được xác định bằng ELISA. KẾT QUẢ Chỉ có nitrat hóa protein tương quan với kích thước của phân nhóm ở lupus và hội chứng Sjogren. KẾT LUẬN Các kết quả này ủng hộ vai trò của nitrat hóa protein trong kích thước phân nhóm và mức độ nghiêm trọng của đợt bùng phát lupus. Nitrat hóa protein cũng có thể góp phần vào sự hình thành tự kháng thể ở hội chứng Sjogren."} +{"idx": 2000061, "en": "Layered zeolite precursors were successfully exfoliated by brief shearing or sonication with the assistance of commercially available telechelic liquid polybutadienes at room temperature. The exfoliated zeolite nanosheets can form a stable suspension in an organic solvent, providing exciting potential for the fabrication of zeolite membranes, composite materials and hierarchical zeolites.", "gemini": "Các tiền chất zeolit nhiều lớp đã được tẩy thành công bằng cách cắt ngắn hoặc siêu âm với sự hỗ trợ của các chất lỏng polybutadien telechelic có sẵn trên thị trường ở nhiệt độ phòng. Các nanosheet zeolit đã tẩy có thể tạo thành một hệ huyền phù ổn định trong dung môi hữu cơ, tạo ra tiềm năng thú vị cho việc chế tạo màng zeolit, vật liệu composite và zeolit phân cấp."} +{"idx": 2000062, "en": "Herein, CO2 chemisorption on SrTiO3(001) surfaces is studied using ab initio calculations to establish new chemical sensing mechanisms. It was found that CO2 adsorption opens the band gap of the material. However, the mechanisms are different: the CO2 adsorption on the TiO2-terminated surface neutralizes the surface states at the valence band (VB) maximum, whereas for the SrO-terminated surface it suppresses the conduction band (CB) minimum. For the TiO2-terminated surface, the effect is explained by the passivation of dangling bonds, whereas for the SrO-terminated surface, the suppression is caused by surface relaxation. Modulation of the VB states implies a more direct change in charge distribution, and thus, the induced change in the band gap is more prominent at the TiO2 termination. Further, it has been shown that both CO2 adsorption energy and surface band gap are strongly dependent on CO2 coverage, suggesting that the observed effect can be utilized in sensing applications for a wide range of CO2 concentrations.", "gemini": "Ở đây, sự hấp phụ hóa học CO2 trên bề mặt SrTiO3(001) được nghiên cứu bằng các phép tính ab initio để thiết lập các cơ chế cảm biến hóa học mới. Người ta thấy rằng sự hấp phụ CO2 mở ra khoảng trống băng của vật liệu. Tuy nhiên, các cơ chế là khác nhau: sự hấp phụ CO2 trên bề mặt kết thúc TiO2 trung hòa các trạng thái bề mặt ở mức tối đa của dải hóa trị (VB), trong khi đối với bề mặt kết thúc SrO, nó ức chế mức tối thiểu của dải dẫn (CB). Đối với bề mặt kết thúc TiO2, hiệu ứng này được giải thích bằng sự thụ động của các liên kết treo, trong khi đối với bề mặt kết thúc SrO, sự ức chế là do sự giãn nở bề mặt. Sự điều chế các trạng thái VB ngụ ý một sự thay đổi trực tiếp hơn trong phân bố điện tích, do đó, sự thay đổi được tạo ra trong khoảng trống băng là nổi bật hơn ở phần kết thúc TiO2. Hơn nữa, đã được chứng minh rằng cả năng lượng hấp phụ CO2 và khoảng trống băng bề mặt đều phụ thuộc mạnh vào độ phủ CO2, cho thấy rằng hiệu ứng quan sát được có thể được sử dụng trong các ứng dụng cảm biến cho nhiều nồng độ CO2."} +{"idx": 2000063, "en": "In situ generated cationic zirconium complexes stabilized by amine-bridged bis(phenolato) ligands have been developed to catalyse C(sp3)-H addition of alkyl pyridines to olefins, which are the first examples of group 4 metal based catalysts in this transformation. Ligand-controlled regioselectivity was observed, which was verified by DFT study.", "gemini": "Các phức chất zirconium cation được tạo ra tại chỗ được ổn định bởi các phối tử bis(phenolato) được nối cầu amin đã được phát triển để xúc tác cho phản ứng cộng C(sp3)-H của alkyl pyridin với olefin, đây là những ví dụ đầu tiên về chất xúc tác dựa trên kim loại nhóm 4 trong quá trình chuyển hóa này. Độ chọn lọc vùng được kiểm soát bởi phối tử đã được quan sát, điều này đã được xác minh bằng nghiên cứu DFT."} +{"idx": 2000064, "en": "Carpatizine (1), a new bridged oxazine derivative, was isolated from a marine-derived Streptomyces strain SNE-011. The structure was fully determined by spectroscopic analysis, ECD calculations and chemical methods. A plausible non-enzymatic reaction mechanism from daryamide D leading to carpatizine was presented, which was confirmed by chemical transformation.", "gemini": "Carpatizine (1), một dẫn xuất oxazine nối cầu mới, đã được phân lập từ chủng Streptomyces nguồn gốc từ biển SNE-011. Cấu trúc được xác định đầy đủ bằng phân tích quang phổ, tính toán ECD và các phương pháp hóa học. Một cơ chế phản ứng không có enzyme hợp lý từ daryamide D dẫn đến carpatizine đã được trình bày, điều này đã được xác nhận bằng chuyển đổi hóa học."} +{"idx": 2000065, "en": "Here we report the innovation of using hexagonal boron nitride (h-BN) nanosheets as the first background-free matrix among the full laser pulse energy range for the analysis and imaging of small molecule metabolites and as an adsorbent for the enrichment of samples. Due to its totally free background and high efficiency as an adsorbent and matrix, h-BN has promising prospects in metabolomics, MALDI imaging, and environmental analysis.", "gemini": "Ở đây chúng tôi báo cáo về sự đổi mới khi sử dụng các tấm nano nitride bo lục giác (h-BN) làm ma trận không nền đầu tiên trong toàn bộ dải năng lượng xung laser để phân tích và hình ảnh các chất chuyển hóa phân tử nhỏ và làm chất hấp phụ để làm giàu mẫu. Do nền hoàn toàn không có và hiệu suất cao như một chất hấp phụ và ma trận, h-BN có triển vọng đầy hứa hẹn trong lĩnh vực chuyển hóa, hình ảnh MALDI và phân tích môi trường."} +{"idx": 2000066, "en": "Iron (Fe) is required for plant health, but it can also be toxic when present in excess. Therefore, Fe levels must be tightly controlled. The Arabidopsis thaliana E3 ligase BRUTUS (BTS) is involved in the negative regulation of the Fe deficiency response and we show here that the two A. thaliana BTS paralogs, BTS LIKE1 (BTSL1) and BTS LIKE2 (BTSL2) encode proteins that act redundantly as negative regulators of the Fe deficiency response. Loss of both of these E3 ligases enhances tolerance to Fe deficiency. We further generated a triple mutant with loss of both BTS paralogs and a partial loss of BTS expression that exhibits even greater tolerance to Fe-deficient conditions and increased Fe accumulation without any resulting Fe toxicity effects. Finally, we identified a mutant carrying a novel missense mutation of BTS that exhibits an Fe deficiency response in the root when grown under both Fe-deficient and Fe-sufficient conditions, leading to Fe toxicity when plants are grown under Fe-sufficient conditions.", "gemini": "Sắt (Fe) là cần thiết cho sức khỏe của cây, nhưng nó cũng có thể trở nên độc hại khi có quá nhiều. Do đó, mức Fe phải được kiểm soát chặt chẽ. Men ligase E3 BRUTUS (BTS) của Arabidopsis thaliana tham gia vào quá trình điều hòa tiêu cực của phản ứng thiếu Fe và chúng tôi chỉ ra ở đây rằng hai paralog BTS của A. thaliana, BTS LIKE1 (BTSL1) và BTS LIKE2 (BTSL2) mã hóa các protein hoạt động dự phòng như các chất điều hòa tiêu cực của phản ứng thiếu Fe. Sự mất mát của cả hai men ligase E3 này làm tăng khả năng chịu đựng đối với tình trạng thiếu Fe. Hơn nữa, chúng tôi đã tạo ra một đột biến ba với sự mất mát của cả hai paralog BTS và một phần mất biểu hiện BTS biểu hiện khả năng chịu đựng thậm chí còn lớn hơn đối với các điều kiện thiếu Fe và tăng tích lũy Fe mà không có bất kỳ tác dụng độc hại nào của Fe. Cuối cùng, chúng tôi đã xác định được một đột biến mang đột biến sai nghĩa mới của BTS biểu hiện phản ứng thiếu Fe ở rễ khi được nuôi cấy trong cả điều kiện thiếu Fe và đủ Fe, dẫn đến độc tính Fe khi cây được nuôi cấy trong điều kiện đủ Fe."} +{"idx": 2000067, "en": "Long single-walled carbon nanotubes, with lengths >10 μm, can be spontaneously dissolved by stirring in a sodium naphthalide N,N-dimethylacetamide solution, yielding solutions of individualised nanotubide ions at concentrations up to 0.74 mg mL-1. This process was directly compared to ultrasonication and found to be less damaging while maintaining greater intrinsic length, with increased individualisation, yield, and concentration. Nanotubide solutions were spun into fibres using a new reactive coagulation process, which covalently grafts a poly(vinyl chloride) matrix to the nanotubes directly at the point of fibre formation. The grafting process insulated the nanotubes electrically, significantly enhancing the dielectric constant to 340% of the bulk polymer. For comparison, samples were prepared using both Supergrowth nanotubes and conventional shorter commercial single-walled carbon nanotubes. The resulting nanocomposites showed similar, high loadings (ca. 20 wt%), but the fibres formed with Supergrowth nanotubes showed significantly greater failure strain (up to ∼25%), and hence more than double the toughness (30.8 MJ m-3), compared to composites containing typical ∼1 μm SWCNTs.", "gemini": "Các ống nano cacbon dài một thành, có chiều dài> 10 μm, có thể tự hòa tan bằng cách khuấy trong dung dịch natri naphthalide N,N-dimethylacetamide, tạo ra các dung dịch ion nanotubide riêng lẻ ở nồng độ lên đến 0,74 mg mL-1. Quá trình này được so sánh trực tiếp với siêu âm và thấy rằng ít gây hại hơn trong khi vẫn duy trì chiều dài cố hữu lớn hơn, với sự cá thể hóa, năng suất và nồng độ tăng lên. Các dung dịch nanotubide được kéo thành sợi bằng cách sử dụng một quy trình đông tụ phản ứng mới, quy trình này ghép trực tiếp ma trận poly (vinyl clorua) vào các ống nano ngay tại điểm hình thành sợi. Quá trình ghép cách điện các ống nano, làm tăng đáng kể hằng số điện môi lên 340% so với polyme khối. Để so sánh, các mẫu đã được chuẩn bị bằng cả ống nano Supergrowth và ống nano cacbon thành một lớp thương mại thông thường ngắn hơn. Các nanocomposite thu được cho thấy tải trọng cao tương tự (khoảng 20 wt%), nhưng các sợi được hình thành với ống nano Supergrowth cho thấy độ biến dạng phá hủy lớn hơn đáng kể (lên đến ∼25%), và do đó độ dẻo dai cao hơn gấp đôi (30,8 MJ m-3), so với các composite chứa điển hình ∼1 μm SWCNT."} +{"idx": 2000068, "en": "For the development of K+-responsive RNA aptamers, we proposed a new general strategy that makes use of a G-quadruplex formation in response to K+. This is the first report of developing an RNA aptamer that demonstrates ON/OFF switching of its target-binding activity by sensing the addition/removal of K+.", "gemini": "Để phát triển các aptamer RNA đáp ứng K+, chúng tôi đề xuất một chiến lược chung mới sử dụng sự hình thành G-quadruplex để đáp ứng với K+. Đây là báo cáo đầu tiên về việc phát triển một aptamer RNA chứng minh sự chuyển đổi bật/tắt hoạt động liên kết mục tiêu của nó bằng cách cảm nhận sự bổ sung/loại bỏ K+."} +{"idx": 2000069, "en": "We have discovered a rare example of a non-LDH cationic inorganic layered material [Cu4(OH)6][O3S(CH2)4SO3]. Its in situ exfoliation achieves record high adsorption capacities for chromate and dichromate. This phenomenon is ascribed to the combination of the strong host-anion electrostatic interactions and the high surface area of our cuprate material, which overcomes the memory effect problem of LDHs. Moreover, our material also selectively captures CrVI with a negligible decrease in capacity when non-toxic, monovalent anions are present.", "gemini": "Chúng tôi đã phát hiện ra một ví dụ hiếm hoi về vật liệu vô cơ xếp lớp cation không phải LDH [Cu4(OH)6][O3S(CH2)4SO3]. Sự tẩy lớp tại chỗ của nó đạt được khả năng hấp phụ cao kỷ lục đối với cromat và đicromat. Hiện tượng này được quy cho sự kết hợp của các tương tác tĩnh điện chủ-anion mạnh và diện tích bề mặt cao của vật liệu cuprat của chúng tôi, giúp khắc phục vấn đề hiệu ứng bộ nhớ của LDH. Hơn nữa, vật liệu của chúng tôi cũng bắt giữ CrVI một cách chọn lọc với sự giảm không đáng kể về khả năng khi có các anion đơn hóa trị không độc hại."} +{"idx": 2000070, "en": "The amorphous state plays a key role in porous coordination polymer and metal-organic framework phase transitions. We investigate a crystalline-to-amorphous-to-amorphous-to-crystalline (CAAC) phase transition in a Zn based coordination polymer, by X-ray absorption fine structure (XAFS) and X-ray pair distribution function (PDF) analysis. We show that the system shows two distinct amorphous phases upon heating. The first involves a reversible transition to a desolvated form of the original network, followed by an irreversible transition to an intermediate phase which has elongated Zn-I bonds.", "gemini": "Trạng thái vô định hình đóng vai trò quan trọng trong quá trình chuyển pha của polyme phối hợp xốp và khung kim loại hữu cơ. Chúng tôi nghiên cứu quá trình chuyển pha từ tinh thể sang vô định hình sang vô định hình sang tinh thể (CAAC) trong một polyme phối hợp gốc Zn, bằng phân tích cấu trúc hấp thụ tia X tinh tế (XAFS) và hàm phân bố cặp tia X (PDF). Chúng tôi chỉ ra rằng hệ thống cho thấy hai pha vô định hình riêng biệt khi gia nhiệt. Đầu tiên liên quan đến quá trình chuyển đổi có thể đảo ngược sang dạng tách dung môi của mạng ban đầu, sau đó là quá trình chuyển đổi không thể đảo ngược sang pha trung gian có liên kết Zn-I kéo dài."} +{"idx": 2000071, "en": "Atmospheric aerosol particles of sizes less than 2.5 microns affect public health in cities. Understanding the fine structures of aerosol particles is important to decipher their source. Here, we use a novel spectroscopic and mechanical microscopy technique of 10 nm spatial resolution to reveal the nanoscale structures of individual aerosol particles.", "gemini": "Các hạt aerosol trong khí quyển có kích thước nhỏ hơn 2,5 micron ảnh hưởng đến sức khỏe cộng đồng ở các thành phố. Việc hiểu được cấu trúc tinh vi của các hạt aerosol rất quan trọng để giải mã nguồn gốc của chúng. Ở đây, chúng tôi sử dụng một kỹ thuật kính hiển vi quang phổ và cơ học mới có độ phân giải không gian 10 nm để tiết lộ cấu trúc ở cấp độ nano của từng hạt aerosol."} +{"idx": 2000072, "en": "Although manganese (Mn) is critical for the proper functioning of various metabolic enzymes and cofactors, excess Mn in the brain causes neurotoxicity. While the exact transport mechanism of Mn has not been fully understood, several importers and exporters for Mn have been identified over the past decade. In addition to Mn-specific transporters, it has been demonstrated that iron transporters can mediate Mn transport in the brain and peripheral tissues. However, while the expression of iron transporters is regulated by body iron stores, whether or not disorders of iron metabolism modify Mn homeostasis has not been systematically discussed. The present review will provide an update on the role of altered iron status in the transport and toxicity of Mn.", "gemini": "Mặc dù mangan (Mn) rất quan trọng đối với hoạt động bình thường của nhiều enzyme và cofactor chuyển hóa, nhưng lượng Mn dư thừa trong não gây ra độc tính thần kinh. Trong khi cơ chế vận chuyển chính xác của Mn vẫn chưa được hiểu đầy đủ, một số chất nhập khẩu và xuất khẩu đối với Mn đã được xác định trong thập kỷ qua. Ngoài các chất vận chuyển Mn đặc hiệu, người ta đã chứng minh rằng các chất vận chuyển sắt có thể trung gian vận chuyển Mn trong não và các mô ngoại biên. Tuy nhiên, trong khi biểu hiện của các chất vận chuyển sắt được điều chỉnh bởi các kho dự trữ sắt trong cơ thể, thì các rối loạn chuyển hóa sắt có làm thay đổi cân bằng Mn hay không vẫn chưa được thảo luận một cách có hệ thống. Bài đánh giá hiện tại sẽ cung cấp thông tin cập nhật về vai trò của tình trạng sắt bị thay đổi trong quá trình vận chuyển và độc tính của Mn."} +{"idx": 2000073, "en": "Biomineralisation is essential for biology. Specialist proteins use peptide motifs that catalyse mineral deposition into nano-to-microscale inorganic materials. Unlike in native proteins, the motifs incorporated into self-assembled fibres can persistently propagate on the microscopic scale enabling empirically defined silica nanostructures. Herein we show that the two main modes of motif templating - linear and orthogonal - in self-assembling, fibre-forming peptide sequences effectively silicify protein fibres. We show that the mere charge and morphology of protein fibres are not sufficient for silica deposition, but it is the synergy between fibrillogenesis and silica-specific motifs regularly spaced in fibres that ensures silica templating, regardless of the relative orientation of the motifs.", "gemini": "Khoáng hóa sinh học rất cần thiết cho sinh học. Các protein chuyên biệt sử dụng các mô típ peptide xúc tác sự lắng đọng khoáng chất thành các vật liệu vô cơ ở quy mô nano đến vi mô. Không giống như trong các protein bản địa, các mô típ được kết hợp vào các sợi tự lắp ráp có thể liên tục lan truyền ở quy mô vi mô cho phép các cấu trúc nano silic được xác định theo kinh nghiệm. Tại đây, chúng tôi chỉ ra rằng hai chế độ chính của mô típ tạo mẫu - tuyến tính và trực giao - trong các trình tự peptide tạo sợi tự lắp ráp làm silic hóa hiệu quả các sợi protein. Chúng tôi chỉ ra rằng điện tích và hình thái của các sợi protein không đủ để lắng đọng silica, nhưng đó là sự hiệp đồng giữa quá trình tạo sợi và các mô típ đặc hiệu của silica được phân bố đều trong các sợi đảm bảo tạo mẫu silica, bất kể hướng tương đối của các mô típ."} +{"idx": 2000074, "en": "A pesticide vapor sensor was developed using an agarose gel-based chip containing a nanopore sensing system. Vaporized omethoate was detected by the absorption into the gel, the complex formation with a DNA aptamer, and its obstruction at the nanopore. This strategy is applicable to other vapors, expanding the versatility of nanopore sensors.", "gemini": "Một cảm biến hơi thuốc trừ sâu đã được phát triển bằng cách sử dụng một chip dựa trên gel agarose chứa hệ thống cảm biến nanopore. Omethoate bay hơi được phát hiện bằng cách hấp thụ vào gel, phức hợp hình thành với aptamer DNA và sự cản trở của nó tại nanopore. Chiến lược này có thể áp dụng cho các loại hơi khác, mở rộng tính linh hoạt của các cảm biến nanopore."} +{"idx": 2000075, "en": "Previously, we showed that an oligodeoxynucleotide with AAAG repeats (AAAG ODN) rescued mice from fatal acute lung injury (ALI) induced by influenza virus and inhibited production of tumor necrosis factor-α (TNF-α) in the injured lungs. However, the underlying mechanisms remain to be elucidated. Upon the bioinformatic analysis revealing that the AAAG ODN is consensus to interferon regulatory factor 5 (IRF5) binding site in the cis-regulatory elements of proinflammatory cytokines, we tried to explore whether the AAAG ODN could attenuate burn injury induced systemic inflammatory responses via inhibiting IRF5 pathway. Using the mouse model with sterile systemic inflammation induced by burn injury, we found that AAAG ODN prolonged the life span of the mice, decreased the expression of IRF5 at injured skin, reduced the production of TNF-α and IL-6 in blood and injured skin, and attenuated the ALI. Furthermore, AAAG ODN could bind IRF5 and inhibit the nuclear translocation of IRF5 in THP-1 cells. The data suggested that the AAAG ODN could act as a cytoplasmic decoy capable of interfering the function of IRF5, and be developed as a drug candidate for the treatment of inflammatory diseases.", "gemini": "Trước đây, chúng tôi đã chứng minh rằng một oligodeoxynucleotide với các đoạn lặp lại AAAG (AAAG ODN) đã cứu những con chuột khỏi tình trạng tổn thương phổi cấp tính gây tử vong (ALI) do vi-rút cúm gây ra và ức chế sản xuất yếu tố hoại tử khối u-α (TNF-α) ở phổi bị tổn thương. Tuy nhiên, các cơ chế cơ bản vẫn chưa được làm sáng tỏ. Sau khi phân tích sinh tin học cho thấy AAAG ODN thống nhất với vị trí liên kết của yếu tố điều hòa interferon 5 (IRF5) trong các yếu tố điều hòa cis của cytokine tiền viêm, chúng tôi đã cố gắng khám phá xem AAAG ODN có thể làm giảm đáp ứng viêm toàn thân do bỏng gây ra thông qua việc ức chế con đường IRF5 hay không. Sử dụng mô hình chuột bị viêm toàn thân vô trùng do bỏng gây ra, chúng tôi thấy rằng AAAG ODN kéo dài tuổi thọ của chuột, làm giảm biểu hiện của IRF5 ở da bị tổn thương, làm giảm sản xuất TNF-α và IL-6 trong máu và da bị tổn thương, và làm giảm ALI. Hơn nữa, AAAG ODN có thể liên kết với IRF5 và ức chế sự dịch chuyển hạt nhân của IRF5 trong tế bào THP-1. Dữ liệu cho thấy rằng AAAG ODN có thể hoạt động như một mồi nhử trong tế bào chất có khả năng can thiệp vào chức năng của IRF5 và được phát triển như một ứng cử viên thuốc để điều trị các bệnh viêm."} +{"idx": 2000076, "en": "Pyrrolizidine alkaloids (PAs) are among the most potent phytotoxins widely distributed in plant species around the world. PA is one of the major causes responsible for the development of hepatic sinusoidal obstruction syndrome (HSOS) and exerts hepatotoxicity via metabolic activation to form the reactive metabolites, which bind with cellular proteins to generate pyrrole-protein adducts, leading to hepatotoxicity. PA N-oxides coexist with their corresponding PAs in plants with varied quantities, sometimes even higher than that of PAs, but the toxicity of PA N-oxides remains unclear. The current study unequivocally identified PA N-oxides as the sole or predominant form of PAs in 18 Gynura segetum herbal samples ingested by patients with liver damage. For the first time, PA N-oxides were recorded to induce HSOS in human. PA N-oxide-induced hepatotoxicity was further confirmed on mice orally dosed of herbal extract containing 170 μmol PA N-oxides/kg/day, with its hepatotoxicity similar to but potency much lower than the corresponding PAs. Furthermore, toxicokinetic study after a single oral dose of senecionine N-oxide (55 μmol/kg) on rats revealed the toxic mechanism that PA N-oxides induced hepatotoxicity via their biotransformation to the corresponding PAs followed by the metabolic activation to form pyrrole-protein adducts. The remarkable differences in toxicokinetic profiles of PAs and PA N-oxides were found and attributed to their significantly different hepatotoxic potency. The findings of PA N-oxide-induced hepatotoxicity in humans and rodents suggested that the contents of both PAs and PA N-oxides present in herbs and foods should be regulated and controlled in use.", "gemini": "Ancaloit pyrrolizidine (PA) là một trong những độc tố thực vật mạnh nhất phân bố rộng rãi trong các loài thực vật trên khắp thế giới. PA là một trong những nguyên nhân chính gây ra hội chứng tắc nghẽn xoang gan (HSOS) và gây độc cho gan thông qua quá trình hoạt hóa chuyển hóa để tạo thành các chất chuyển hóa phản ứng, liên kết với protein tế bào để tạo thành các chất kết hợp pyrrole-protein, dẫn đến độc tính cho gan. PA N-oxit cùng tồn tại với PA tương ứng của chúng trong thực vật với số lượng khác nhau, đôi khi thậm chí cao hơn PA, nhưng độc tính của PA N-oxit vẫn chưa rõ ràng. Nghiên cứu hiện tại đã xác định rõ ràng PA N-oxit là dạng duy nhất hoặc chiếm ưu thế của PA trong 18 mẫu thảo dược Gynura segetum mà bệnh nhân bị tổn thương gan đã ăn vào. Lần đầu tiên, PA N-oxit được ghi nhận gây ra HSOS ở người. Độc tính với gan do PA N-oxit gây ra đã được xác nhận thêm trên chuột được uống chiết xuất thảo dược có chứa 170 μmol PA N-oxit/kg/ngày, với độc tính với gan tương tự nhưng độc lực thấp hơn nhiều so với PA tương ứng. Hơn nữa, nghiên cứu độc động học sau một liều uống duy nhất senecionine N-oxit (55 μmol/kg) trên chuột đã tiết lộ cơ chế độc hại mà PA N-oxit gây ra độc tính cho gan thông qua quá trình chuyển hóa sinh học của chúng thành PA tương ứng sau đó là quá trình hoạt hóa chuyển hóa để tạo thành các chất kết hợp pyrrole-protein. Sự khác biệt đáng kể về hồ sơ độc động học của PA và PA N-oxit đã được tìm thấy và được quy cho độc lực gây độc cho gan khác biệt đáng kể của chúng. Những phát hiện về độc tính với gan do PA N-oxit gây ra ở người và động vật gặm nhấm cho thấy rằng hàm lượng PA và PA N-oxit có trong thảo mộc và thực phẩm nên được kiểm soát và kiểm soát khi sử dụng."} +{"idx": 2000077, "en": "BACKGROUND Even though most patients who undergo resection of pancreatic adenocarcinoma have T3 disease with extra-pancreatic tumor extension, T3 disease is not currently classified by tumor size. The aim of this study was to modify the current TNM classification of pancreatic adenocarcinoma to reflect the influence of tumor size. METHODS A total of 847 consecutive pancreatectomy patients were recruited from multiple centers. Optimum tumor size cutoff values were calculated by receiver operating characteristics analysis for tumors limited to the pancreas (T1/2) and for T3 tumors. In our modified TNM classification, stage II was divided into stages IIA (T3aN0M0), IIB (T3bN0M0), and IIC (T1-3bN1M0) using tumor size cutoff values. The usefulness of the new classification was compared with that of the current classification using Akaike's information criterion (AIC). RESULTS The optimum tumor size cutoff value distinguishing T1 and T2 was 2 cm, while T3 was divided into T3a and T3b at a tumor size of 3 cm. The median survival time of the stages IIA, IIB, and IIC were 44.7, 27.6, and 20.3 months, respectively. There were significant differences of survival between stages IIA and IIB (P = 0.02) and between stages IIB and IIC (P = 0.03). The new classification showed better performance compared with the current classification based on the AIC value. CONCLUSIONS This proposed new TNM classification reflects the influence of tumor size in patients with extra-pancreatic tumor extension (T3 disease), and the classification is useful for predicting mortality.", "gemini": "LÝ LỊCH Mặc dù hầu hết bệnh nhân cắt bỏ ung thư biểu mô tuyến tụy đều mắc bệnh T3 với tình trạng khối u lan ra ngoài tuyến tụy, nhưng bệnh T3 hiện không được phân loại theo kích thước khối u. Mục đích của nghiên cứu này là sửa đổi phân loại TNM hiện tại của ung thư biểu mô tuyến tụy để phản ánh ảnh hưởng của kích thước khối u. PHƯƠNG PHÁP Tổng cộng 847 bệnh nhân cắt tụy liên tiếp đã được tuyển dụng từ nhiều trung tâm. Các giá trị ngưỡng kích thước khối u tối ưu được tính bằng phân tích đặc điểm hoạt động của máy thu cho các khối u giới hạn ở tuyến tụy (T1/2) và đối với các khối u T3. Trong phân loại TNM đã sửa đổi của chúng tôi, giai đoạn II được chia thành giai đoạn IIA (T3aN0M0), IIB (T3bN0M0) và IIC (T1-3bN1M0) bằng cách sử dụng các giá trị ngưỡng kích thước khối u. Tính hữu ích của phân loại mới đã được so sánh với phân loại hiện tại bằng cách sử dụng tiêu chuẩn thông tin của Akaike (AIC). KẾT QUẢ Giá trị ngưỡng kích thước khối u tối ưu để phân biệt T1 và T2 là 2 cm, trong khi T3 được chia thành T3a và T3b ở kích thước khối u là 3 cm. Thời gian sống sót trung bình của các giai đoạn IIA, IIB và IIC lần lượt là 44,7, 27,6 và 20,3 tháng. Có sự khác biệt đáng kể về tỷ lệ sống sót giữa giai đoạn IIA và IIB (P = 0,02) và giữa giai đoạn IIB và IIC (P = 0,03). Phân loại mới cho thấy hiệu suất tốt hơn so với phân loại hiện tại dựa trên giá trị AIC. KẾT LUẬN Phân loại TNM mới được đề xuất này phản ánh ảnh hưởng của kích thước khối u ở những bệnh nhân bị khối u lan ra ngoài tuyến tụy (bệnh T3) và phân loại này hữu ích để dự đoán tỷ lệ tử vong."} +{"idx": 2000078, "en": "The aim of this study was to assess the effect of peloid on pain, functionality, daily life activities, and quality of life of lateral epicondylitis (LE) patients. In this randomized, controlled, single-blind study, 75 patients who were diagnosed with chronic LE were enrolled to the study. Patients were randomized into two groups using the random number table. The patients in the first group (group 1) (n = 33), were given lateral epicondylitis band (LEB) (during the day for 6 weeks) + peloid therapy (five consecutive days a week for 2 weeks), and the second group (group 2) (n = 32), received LEB treatment alone. The patients were assessed by using Patient Rated Tennis Elbow Evaluation (PRTEE) and Nottingham Health Profile (NHP). The data were obtained before treatment (W0), immediately after treatment (W2), and 1 month after treatment (W6). In analysis of the collected data, the Wilcoxon signed rank test for intra-group comparisons and Mann-Whitney U test for comparisons between groups were used. Both in groups 1 and 2, there was a statistically significant improvement in all the evaluation parameters at W2 and W6 when compared to W0 (p < 0.05). In comparison of difference scores between groups, although there was no statistically significant difference between the two groups at W2 when compared to W0 (p > 0.05), a statistically significant difference was found in favor of group 1 for all the evaluation parameters at W6 (p < 0.05). Our results have shown that peloid treatment could be effective in providing improvement in pain relief, function, daily life activities, and quality of life in LE patients.", "gemini": "Mục đích của nghiên cứu này là đánh giá tác dụng của peloid đối với cơn đau, chức năng, các hoạt động trong cuộc sống hàng ngày và chất lượng cuộc sống của bệnh nhân viêm mỏm ngoài lồi (LE). Trong nghiên cứu ngẫu nhiên, có đối chứng, mù đơn này, 75 bệnh nhân được chẩn đoán mắc bệnh LE mạn tính đã được đưa vào nghiên cứu. Bệnh nhân được chia ngẫu nhiên thành hai nhóm bằng cách sử dụng bảng số ngẫu nhiên. Những bệnh nhân ở nhóm thứ nhất (nhóm 1) (n = 33), được dùng băng viêm mỏm ngoài lồi (LEB) (vào ban ngày trong 6 tuần) + liệu pháp peloid (năm ngày liên tiếp một tuần trong 2 tuần), và nhóm thứ hai (nhóm 2) (n = 32), chỉ được điều trị bằng LEB. Bệnh nhân được đánh giá bằng cách sử dụng Đánh giá khuỷu tay chơi quần vợt do bệnh nhân đánh giá (PRTEE) và Hồ sơ sức khỏe Nottingham (NHP). Dữ liệu được thu thập trước khi điều trị (W0), ngay sau khi điều trị (W2) và 1 tháng sau khi điều trị (W6). Trong phân tích dữ liệu thu thập được, kiểm định Wilcoxon signed rank được sử dụng cho các so sánh trong nhóm và kiểm định Mann-Whitney U được sử dụng cho các so sánh giữa các nhóm. Ở cả nhóm 1 và 2, có sự cải thiện có ý nghĩa thống kê về tất cả các thông số đánh giá tại W2 và W6 khi so sánh với W0 (p < 0,05). Khi so sánh điểm chênh lệch giữa các nhóm, mặc dù không có sự khác biệt có ý nghĩa thống kê giữa hai nhóm tại W2 khi so sánh với W0 (p > 0,05), nhưng có sự khác biệt có ý nghĩa thống kê có lợi cho nhóm 1 đối với tất cả các thông số đánh giá tại W6 (p < 0,05). Kết quả của chúng tôi đã chỉ ra rằng phương pháp điều trị bằng peloid có thể có hiệu quả trong việc cải thiện giảm đau, chức năng, các hoạt động trong cuộc sống hàng ngày và chất lượng cuộc sống ở bệnh nhân LE."} +{"idx": 2000079, "en": "AIMS While lifestyle modification is known to offer several metabolic benefits, there is paucity of comprehensive data on changes in biomarkers of adiposity, inflammation as well as gut hormones. We investigated these biomarkers in overweight/obese individuals with prediabetes randomized to either 4 months of a lifestyle improvement program or standard care and followed them up for a year. METHODS Participants [standard care and intervention arm (n = 75 each)] were randomly selected from the Diabetes Community Lifestyle Improvement Program trial. Glycemic and lipid control and anthropometric measurements were assessed by standard protocols. Adipokines, inflammatory markers and gut hormones were measured using multiplex and standard ELISA kits. RESULTS Along with modest benefits in primary outcomes (glycemic and lipid control and weight reduction), participants in the intervention group showed significant reductions (p < 0.001) in plasma levels of leptin (17.6%), TNF-α (35%), IL-6 (33.3%), MCP-1 (22.3%) and PYY (28.3%) and increased levels of adiponectin (33.1%) and ghrelin (23.6%) at the end of 4 months of lifestyle intervention. The changes were independent of weight and persisted even at 1 year of follow-up. In contrast, participants from the standard care arm did not show any statistically significant improvements on the above parameters. CONCLUSIONS Participants who underwent an intensive lifestyle improvement program showed metabolic benefits as well as favorable beneficial changes in systemic levels of adipokines, cytokines and gut hormones, not only during the intervention period, but also during 12-month follow-up period.", "gemini": "MỤC ĐÍCH Mặc dù việc thay đổi lối sống được biết là mang lại một số lợi ích về mặt chuyển hóa, nhưng vẫn còn thiếu dữ liệu toàn diện về những thay đổi trong các dấu ấn sinh học của tình trạng béo phì, viêm cũng như các hormone đường ruột. Chúng tôi đã nghiên cứu các dấu ấn sinh học này ở những người thừa cân/béo phì mắc tiền tiểu đường được phân ngẫu nhiên vào chương trình cải thiện lối sống trong 4 tháng hoặc chăm sóc tiêu chuẩn và theo dõi họ trong một năm. PHƯƠNG PHÁP Những người tham gia [chăm sóc tiêu chuẩn và nhóm can thiệp (n = 75 mỗi nhóm)] được lựa chọn ngẫu nhiên từ thử nghiệm Chương trình cải thiện lối sống cộng đồng về bệnh tiểu đường. Kiểm soát lipid và đường huyết và các phép đo nhân trắc được đánh giá theo các giao thức chuẩn. Adipokine, dấu ấn viêm và hormone đường ruột được đo bằng bộ dụng cụ ELISA tiêu chuẩn và đa hợp. KẾT QUẢ Cùng với những lợi ích khiêm tốn về các kết quả chính (kiểm soát lipid và đường huyết và giảm cân), những người tham gia trong nhóm can thiệp cho thấy sự giảm đáng kể (p < 0,001) về nồng độ leptin trong huyết tương (17,6%), TNF-α (35%), IL-6 (33,3%), MCP-1 (22,3%) và PYY (28,3%) và tăng nồng độ adiponectin (33,1%) và ghrelin (23,6%) sau 4 tháng can thiệp về lối sống. Những thay đổi này không phụ thuộc vào cân nặng và vẫn tồn tại ngay cả sau 1 năm theo dõi. Ngược lại, những người tham gia trong nhóm chăm sóc tiêu chuẩn không cho thấy bất kỳ cải thiện có ý nghĩa thống kê nào về các thông số trên. KẾT LUẬN Những người tham gia thực hiện chương trình cải thiện lối sống chuyên sâu đã cho thấy những lợi ích về mặt chuyển hóa cũng như những thay đổi có lợi về mặt hệ thống ở mức adipokine, cytokine và hormone đường ruột, không chỉ trong thời gian can thiệp mà còn trong thời gian theo dõi 12 tháng."} +{"idx": 2000080, "en": "Avian influenza viruses (AIV) are of great socioeconomic and health concern, notably in Southeast Asia where highly pathogenic strains, such as highly pathogenic avian influenza (HPAI) H5N1 and other H5 and H7 AIVs, continue to occur. Wild bird migrants are often implicated in the maintenance and spread of AIV. However, little systematic surveillance of wild birds has been conducted in Southeast Asia to evaluate whether the prevalence of AIV in wild birds is higher than in other parts of the world where HPAI outbreaks occur less frequently. Across Bangladesh, we randomly sampled a total of 3585 wild and domestic birds to assess the prevalence of AIV and antibodies against AIV and compared these with prevalence levels found in other endemic and non-endemic countries. Our study showed that both resident and migratory wild birds in Bangladesh do not have a particularly elevated AIV prevalence and AIV sero-prevalence compared to wild birds from regions in the world where H5N1 is not endemic and fewer AIV outbreaks in poultry occur. Like elsewhere, notably wild birds of the orders Anseriformes were identified as the main wild bird reservoir, although we found exceptionally high sero-prevalence in one representative of the order Passeriformes, the house crow (Corvus splendens), importantly living on offal from live bird markets. This finding, together with high sero- and viral prevalence levels of AIV in domestic birds, suggests that wild birds are not at the base of the perpetuation of AIV problems in the local poultry sector, but may easily become victim to AIV spill back from poultry into some species of wild birds, potentially assisting in further spread of the virus.", "gemini": "Virus cúm gia cầm (AIV) là mối quan tâm lớn về mặt kinh tế xã hội và sức khỏe, đặc biệt là ở Đông Nam Á, nơi các chủng virus có độc lực cao, chẳng hạn như cúm gia cầm có độc lực cao (HPAI) H5N1 và các loại AIV H5 và H7 khác, vẫn tiếp tục xảy ra. Các loài chim di trú hoang dã thường có liên quan đến việc duy trì và lây lan AIV. Tuy nhiên, rất ít hoạt động giám sát có hệ thống đối với các loài chim hoang dã được tiến hành ở Đông Nam Á để đánh giá xem tỷ lệ lưu hành của AIV ở các loài chim hoang dã có cao hơn ở các khu vực khác trên thế giới nơi các đợt bùng phát HPAI xảy ra ít thường xuyên hơn hay không. Trên khắp Bangladesh, chúng tôi đã lấy mẫu ngẫu nhiên tổng cộng 3585 loài chim hoang dã và gia cầm để đánh giá tỷ lệ lưu hành của AIV và kháng thể chống lại AIV, đồng thời so sánh chúng với mức độ lưu hành được tìm thấy ở các quốc gia đặc hữu và không đặc hữu khác. Nghiên cứu của chúng tôi cho thấy rằng cả chim hoang trú và chim di trú ở Bangladesh đều không có tỷ lệ lưu hành AIV và tỷ lệ huyết thanh AIV đặc biệt cao so với chim hoang dã từ các khu vực trên thế giới nơi H5N1 không lưu hành và ít xảy ra các đợt bùng phát AIV ở gia cầm hơn. Giống như ở những nơi khác, đặc biệt là các loài chim hoang thuộc bộ Ngỗng được xác định là ổ chứa chính của loài chim hoang dã, mặc dù chúng tôi phát hiện ra tỷ lệ huyết thanh đặc biệt cao ở một đại diện của bộ Sẻ, quạ nhà (Corvus splendens), chủ yếu sống bằng thức ăn thừa từ các chợ chim sống. Phát hiện này, cùng với tỷ lệ lưu hành huyết thanh và vi-rút cao của AIV ở gia cầm, cho thấy rằng chim hoang dã không phải là cơ sở của vấn đề duy trì AIV trong ngành gia cầm địa phương, nhưng có thể dễ dàng trở thành nạn nhân của việc AIV lây lan ngược từ gia cầm vào một số loài chim hoang dã, có khả năng hỗ trợ lây lan vi-rút xa hơn."} +{"idx": 2000081, "en": "We performed a literature review in order to improve our understanding of how landscape and climate drivers affect HCPS outbreaks. Anthropogenic landscape changes such as forest loss, fragmentation and agricultural land uses are related with a boost in hantavirus reservoir species abundance and hantavirus prevalence in tropical areas, increasing HCPS risk. Additionally, higher precipitation, especially in arid regions, favors an increase in vegetational biomass, which augments the resources for reservoir rodents, also increasing HCPS risk. Although these relationships were observed, few studies described it so far, and the ones that did it are concentrated in few places. To guide future research on this issue, we build a conceptual model relating landscape and climate variables with HCPS outbreaks and identified research opportunities. We point out the need for studies addressing the effects of landscape configuration, temperature and the interaction between climate and landscape variables. Critical landscape thresholds are also highly relevant, once HCPS risk transmission can increase rapidly above a certain degree of landscape degradation. These studies could be relevant to implement preventive measures, creating landscapes that can mitigate disease spread risk.", "gemini": "Chúng tôi đã thực hiện một đánh giá tài liệu để nâng cao hiểu biết của chúng tôi về cách các yếu tố thúc đẩy cảnh quan và khí hậu ảnh hưởng đến sự bùng phát HCPS. Những thay đổi về cảnh quan do con người gây ra như mất rừng, chia cắt và sử dụng đất nông nghiệp có liên quan đến sự gia tăng số lượng loài chứa hantavirus và tỷ lệ lưu hành hantavirus ở các khu vực nhiệt đới, làm tăng nguy cơ HCPS. Ngoài ra, lượng mưa cao hơn, đặc biệt là ở các vùng khô cằn, tạo điều kiện thuận lợi cho sự gia tăng sinh khối thực vật, làm tăng nguồn tài nguyên cho loài gặm nhấm chứa mầm bệnh, đồng thời cũng làm tăng nguy cơ HCPS. Mặc dù các mối quan hệ này đã được quan sát thấy, nhưng rất ít nghiên cứu mô tả về nó cho đến nay và những nghiên cứu đã thực hiện đều tập trung ở một số ít địa điểm. Để hướng dẫn các nghiên cứu trong tương lai về vấn đề này, chúng tôi xây dựng một mô hình khái niệm liên quan đến các biến cảnh quan và khí hậu với các đợt bùng phát HCPS và xác định các cơ hội nghiên cứu. Chúng tôi chỉ ra nhu cầu nghiên cứu giải quyết các tác động của cấu hình cảnh quan, nhiệt độ và sự tương tác giữa các biến khí hậu và cảnh quan. Các ngưỡng cảnh quan quan trọng cũng rất có liên quan, vì nguy cơ lây truyền HCPS có thể tăng nhanh trên một mức độ suy thoái cảnh quan nhất định. Những nghiên cứu này có thể có liên quan đến việc thực hiện các biện pháp phòng ngừa, tạo ra cảnh quan có thể giảm thiểu nguy cơ lây lan bệnh."} +{"idx": 2000082, "en": "Myocardial extracellular volume fraction (ECV) reflecting diffuse myocardial fibrosis can be measured with T1 mapping cardiovascular magnetic resonance (CMR) before and after the application of a gadolinium-based extracellular contrast agent. The equilibrium between blood and myocardium contrast concentration required for ECV measurements can be obtained with a primed contrast infusion (equilibrium contrast-CMR). We hypothesized that equilibrium can also be achieved with a single contrast bolus to accurately measure diffuse myocardial fibrosis in patients with congenital heart disease (CHD). Healthy controls (n = 17; median age 24.0 years) and patients with CHD (n = 19; 25.0 years) were prospectively enrolled. Using modified Look-Locker inversion recovery T1 mapping before, 15 min after bolus injection, and during constant infusion of gadolinium-DOTA, T1 values were obtained for blood pool and myocardium of the left ventricle (LV), the interventricular septum (IVS), and the right ventricle (RV) in a single midventricular plane in short axis or in transverse orientation. ECV of LV, IVS and RV by bolus-only and bolus-infusion correlated significantly in CHD patients (r = 0.94, 0.95, and 0.74; p < 0.01, respectively) and healthy controls (r = 0.96, 0.89, and 0.64; p < 0.05, respectively). Bland-Altman plots revealed no significant bias between the techniques for any of the analyzed regions. ECV of LV and RV myocardium measured by bolus-only T1 mapping agrees well with bolus-infusion measurements in patients with CHD. The use of a bolus-only approach facilitates the integration of ECV measurements into existing CMR imaging protocols, allowing for assessment of diffuse myocardial fibrosis in CHD in clinical routine.", "gemini": "Thể tích ngoại bào cơ tim (ECV) phản ánh xơ hóa cơ tim khuếch tán có thể được đo bằng phương pháp lập bản đồ T1 cộng hưởng từ tim mạch (CMR) trước và sau khi sử dụng thuốc cản quang ngoại bào dựa trên gadolinium. Sự cân bằng giữa nồng độ thuốc cản quang trong máu và cơ tim cần thiết để đo ECV có thể đạt được bằng cách truyền thuốc cản quang đã chuẩn độ (CMR thuốc cản quang cân bằng). Chúng tôi đưa ra giả thuyết rằng sự cân bằng cũng có thể đạt được bằng một liều thuốc cản quang duy nhất để đo chính xác tình trạng xơ hóa cơ tim khuếch tán ở những bệnh nhân mắc bệnh tim bẩm sinh (CHD). Những người đối chứng khỏe mạnh (n = 17; độ tuổi trung bình 24,0 năm) và bệnh nhân mắc CHD (n = 19; 25,0 năm) đã được tuyển dụng có chủ đích. Sử dụng phương pháp lập bản đồ T1 đảo ngược Look-Locker đã sửa đổi trước, 15 phút sau khi tiêm thuốc và trong quá trình truyền liên tục gadolinium-DOTA, các giá trị T1 thu được cho nhóm máu và cơ tim của tâm thất trái (LV), vách liên thất (IVS) và tâm thất phải (RV) trên một mặt phẳng giữa tâm thất ở trục ngắn hoặc theo hướng ngang. ECV của LV, IVS và RV bằng phương pháp chỉ tiêm thuốc và truyền thuốc có tương quan đáng kể ở bệnh nhân CHD (r = 0,94, 0,95 và 0,74; p < 0,01) và những người đối chứng khỏe mạnh (r = 0,96, 0,89 và 0,64; p < 0,05). Các biểu đồ Bland-Altman cho thấy không có độ lệch đáng kể giữa các kỹ thuật đối với bất kỳ vùng nào được phân tích. ECV của cơ tim LV và RV được đo bằng phương pháp lập bản đồ T1 chỉ tiêm thuốc phù hợp với các phép đo truyền thuốc ở những bệnh nhân mắc CHD. Việc sử dụng phương pháp chỉ tiêm thuốc tạo điều kiện thuận lợi cho việc tích hợp các phép đo ECV vào các giao thức chụp CMR hiện có, cho phép đánh giá tình trạng xơ hóa cơ tim khuếch tán ở CHD trong hoạt động lâm sàng."} +{"idx": 2000083, "en": "A profit function for a typical commercial farm of intensive guinea pig production was designed. The simulated farm contained 86 cages with a ratio of 7:1 females/males, with continuous mating. Kits were weaned at 15 days of age and slaughtered for meat production at 90 days of age. The absolute (EW) and relative economic weights of the main traits were calculated. The highest EW were kits produced per kindling (US$25), kits weaned per kindling (US$22), kits born alive per kindling (US$20), and the number of kindlings per female and year (US$12). Profit, returns, and costs per female and year were US$15, 68, and 53, respectively. Returns came from the production of young guinea pigs and discarded reproductive adults for meat production, 90 and 10% of the total returns. The highest costs were feeding and labor, 44 and 23% of the total cost. The EW and profit did not substantially change when simulating variations of ±20% in the prices of kilograms of fattening feed and kilograms of live weight of guinea pig, showing their robustness to future variations in market prices or to variations in prices between countries. The results obtained highlight the importance of the feeding costs in the guinea pig meat production.", "gemini": "Một hàm lợi nhuận cho một trang trại thương mại điển hình sản xuất nhiều lợn guinea đã được thiết kế. Trang trại mô phỏng chứa 86 chuồng với tỷ lệ 7:1 con cái/con đực, giao phối liên tục. Thỏ con được cai sữa ở độ tuổi 15 ngày và giết mổ để sản xuất thịt ở độ tuổi 90 ngày. Trọng số kinh tế tuyệt đối (EW) và tương đối của các đặc điểm chính đã được tính toán. EW cao nhất là số thỏ con sinh ra trên một lứa (25 đô la Mỹ), số thỏ con cai sữa trên một lứa (22 đô la Mỹ), số thỏ con sinh ra sống trên một lứa (20 đô la Mỹ) và số lứa trên một con cái và một năm (12 đô la Mỹ). Lợi nhuận, lợi tức và chi phí trên một con cái và một năm lần lượt là 15, 68 và 53 đô la Mỹ. Lợi tức đến từ việc sản xuất lợn guinea non và những con trưởng thành sinh sản bị loại bỏ để sản xuất thịt, chiếm 90 và 10% tổng lợi tức. Chi phí cao nhất là thức ăn và nhân công, chiếm 44 và 23% tổng chi phí. EW và lợi nhuận không thay đổi đáng kể khi mô phỏng các biến động ± 20% về giá của một kg thức ăn vỗ béo và giá của một kg lợn guinea sống, cho thấy khả năng chống chịu của chúng đối với các biến động trong tương lai về giá thị trường hoặc sự khác biệt về giá giữa các quốc gia. Kết quả thu được nhấn mạnh tầm quan trọng của chi phí thức ăn trong sản xuất thịt lợn guinea."} +{"idx": 2000084, "en": "PURPOSE To compare the survival benefit and safety of the low power cumulative and traditional high intensity focused ultrasound (HIFU) for locally advanced pancreatic cancer. METHOD We retrospectively analyzed 38 patients with locally advanced, inoperable, stage III pancreatic patients received HIFU treatment between January 2008 and April 2014 in the Department of Surgery, the Second Affiliated Hospital, Zhejiang University, School of Medicine. 11 of them received the low power cumulative HIFU treatment, while other 27 received the traditional HIFU treatment. The HIFU device used was the FEP-BY02 (Yuande Biomedical Engineering Co. Ltd, Beijing, China). Serum biochemistry and adverse events were assessed before and after treatment. All the patients were followed up until death. The survival rate and adverse events of two groups were compared. RESULTS In 38 patients, the baseline characteristics including gender, age, Karnofsky performance status (KPS) score, tumor location of two groups were generally well balanced (P > 0.05). The median overall survival (OS) for low power cumulative HIFU group was 10.3 months (95% CI, 6.3-14.3 months), which is significantly longer than traditional HIFU group with 6.0 months (95% CI, 5.2-6.8 months) (P = 0.018). In low power cumulative HIFU group, the 6-month and 12-month survival rates were higher than traditional group, 100% v.s 44.4%, 11.1% v.s 36.4%, respectively. The adverse events in both groups include abdominal pain, fever, C-reactive protein (CRP) elevated. The incidence was lower in low power cumulative HIFU group, however, without statistical significance. CONCLUSION The low power cumulative HIFU treatment showed a statistical significance in survival benefit with better safety profile compared to the traditional HIFU treatment in patients with locally advanced pancreatic cancer.", "gemini": "MỤC ĐÍCH So sánh lợi ích sống còn và tính an toàn của sóng siêu âm hội tụ cường độ thấp tích lũy và sóng siêu âm hội tụ cường độ cao truyền thống (HIFU) đối với ung thư tuyến tụy tiến triển tại chỗ. PHƯƠNG PHÁP Chúng tôi đã phân tích hồi cứu 38 bệnh nhân ung thư tuyến tụy giai đoạn III tiến triển tại chỗ, không thể phẫu thuật đã được điều trị bằng HIFU từ tháng 1 năm 2008 đến tháng 4 năm 2014 tại Khoa Phẫu thuật, Bệnh viện trực thuộc thứ hai, Đại học Chiết Giang, Khoa Y. 11 trong số họ được điều trị bằng HIFU tích lũy năng lượng thấp, trong khi 27 người khác được điều trị bằng HIFU truyền thống. Thiết bị HIFU được sử dụng là FEP-BY02 (Yuande Biomedical Engineering Co. Ltd, Bắc Kinh, Trung Quốc). Sinh hóa huyết thanh và các tác dụng phụ được đánh giá trước và sau khi điều trị. Tất cả các bệnh nhân được theo dõi cho đến khi tử vong. Tỷ lệ sống sót và các tác dụng phụ của hai nhóm được so sánh. KẾT QUẢ Trong số 38 bệnh nhân, các đặc điểm cơ bản bao gồm giới tính, tuổi, điểm trạng thái hoạt động Karnofsky (KPS), vị trí khối u của hai nhóm nói chung cân bằng tốt (P> 0,05). Sự sống sót toàn thể (OS) trung bình đối với nhóm HIFU tích lũy năng lượng thấp là 10,3 tháng (95% CI, 6,3-14,3 tháng), dài hơn đáng kể so với nhóm HIFU truyền thống với 6,0 tháng (95% CI, 5,2-6,8 tháng) (P = 0,018). Trong nhóm HIFU tích lũy năng lượng thấp, tỷ lệ sống sót sau 6 tháng và 12 tháng cao hơn nhóm truyền thống, lần lượt là 100% so với 44,4%, 11,1% so với 36,4%. Các tác dụng phụ ở cả hai nhóm bao gồm đau bụng, sốt, protein phản ứng C (CRP) tăng cao. Tỷ lệ mắc bệnh thấp hơn ở nhóm HIFU tích lũy năng lượng thấp, tuy nhiên không có ý nghĩa thống kê. KẾT LUẬN Điều trị HIFU tích lũy năng lượng thấp cho thấy có ý nghĩa thống kê về lợi ích sống còn với hồ sơ an toàn tốt hơn so với điều trị HIFU truyền thống ở những bệnh nhân ung thư tuyến tụy tiến triển tại chỗ."} +{"idx": 2000085, "en": "Since the discovery that anaerobic methanotrophic archaea (ANME) are involved in the anaerobic oxidation of methane coupled to sulfate reduction in marine sediments, different primers and probes specifically targeting the 16S rRNA gene of these archaea have been developed. Microbial investigation of the different ANME subtypes (ANME-1; ANME-2a, b, and c; and ANME-3) was mainly done in sediments where specific subtypes of ANME were highly enriched and methanogenic cell numbers were low. In different sediments with higher archaeal diversity and abundance, it is important that primers and probes targeting different ANME subtypes are very specific and do not detect other ANME subtypes or methanogens that are also present. In this study, primers and probes that were regularly used in AOM studies were tested in silico on coverage and specificity. Most of the previously developed primers and probes were not specific for the ANME subtypes, thereby not reflecting the actual ANME population in complex samples. Selected primers that showed good coverage and high specificity for the subclades ANME-1, ANME-2a/b, and ANME-2c were thoroughly validated using quantitative polymerase chain reaction (qPCR). From these qPCR tests, only certain combinations seemed suitable for selective amplification. After optimization of these primer sets, we obtained valid primer combinations for the selective detection and quantification of ANME-1, ANME-2a/b, and ANME-2c in samples where different ANME subtypes and possibly methanogens could be present. As a result of this work, we propose a standard workflow to facilitate selection of suitable primers for qPCR experiments on novel environmental samples.", "gemini": "Kể từ khi phát hiện ra rằng vi khuẩn cổ ưa khí metan (ANME) có liên quan đến quá trình oxy hóa metan kỵ khí kết hợp với quá trình khử sulfat trong trầm tích biển, các loại mồi và đầu dò khác nhau nhắm vào gen rRNA 16S của các vi khuẩn cổ này đã được phát triển. Nghiên cứu vi sinh vật về các phân nhóm ANME khác nhau (ANME-1; ANME-2a, b và c; và ANME-3) chủ yếu được thực hiện trong các trầm tích có các phân nhóm ANME cụ thể được làm giàu cao và số lượng tế bào sinh metan thấp. Trong các trầm tích khác nhau có sự đa dạng và phong phú của vi khuẩn cổ cao hơn, điều quan trọng là các loại mồi và đầu dò nhắm vào các phân nhóm ANME khác nhau phải rất đặc hiệu và không phát hiện ra các phân nhóm ANME khác hoặc các vi khuẩn sinh metan cũng có mặt. Trong nghiên cứu này, các loại mồi và đầu dò được sử dụng thường xuyên trong các nghiên cứu AOM đã được thử nghiệm in silico về độ phủ và độ đặc hiệu. Hầu hết các loại mồi và đầu dò đã phát triển trước đó không đặc hiệu đối với các phân nhóm ANME, do đó không phản ánh quần thể ANME thực tế trong các mẫu phức tạp. Các loại mồi được lựa chọn có độ phủ tốt và độ đặc hiệu cao đối với các phân nhóm ANME-1, ANME-2a/b và ANME-2c đã được xác nhận kỹ lưỡng bằng phản ứng chuỗi polymerase định lượng (qPCR). Từ các xét nghiệm qPCR này, chỉ một số kết hợp nhất định có vẻ phù hợp để khuếch đại chọn lọc. Sau khi tối ưu hóa các bộ mồi này, chúng tôi đã thu được các kết hợp mồi hợp lệ để phát hiện và định lượng chọn lọc ANME-1, ANME-2a/b và ANME-2c trong các mẫu có thể có các phân nhóm ANME khác nhau và có thể là các vi khuẩn sinh metan. Kết quả của công trình này, chúng tôi đề xuất một quy trình làm việc chuẩn để tạo điều kiện thuận lợi cho việc lựa chọn các loại mồi phù hợp cho các thí nghiệm qPCR trên các mẫu môi trường mới."} +{"idx": 2000086, "en": "Tryptophan dimers (TDs) are an important class of natural products with diverse bioactivities and share conserved biosynthetic pathways. We report the identification of a partial gene cluster (spm) responsible for the biosynthesis of a class of unusual TDs with non-planar skeletons including spiroindimicins (SPMs), indimicins (IDMs), and lynamicins (LNMs) from the deep-sea derived Streptomyces sp. SCSIO 03032. Bioinformatics analysis, targeted gene disruptions, and heterologous expression studies confirmed the involvement of the spm gene cluster in the biosynthesis of SPM/IDM/LNMs, and revealed the indispensable roles for the halogenase/reductase pair SpmHF, the amino acid oxidase SpmO, and the chromopyrrolic acid (CPA) synthase SpmD, as well as the positive regulator SpmR and the putative transporter SpmA. However, the spm gene cluster was unable to confer a heterologous host the ability to produce SPM/IDM/LNMs. In addition, the P450 enzyme SpmP and the monooxygenase SpmX2 were found to be non-relevant to the biosynthesis of SPM/IDM/LNMs. Sequence alignment and structure modeling suggested the lack of key conserved amino acid residues in the substrate-binding pocket of SpmP. Furthermore, feeding experiments in the non-producing ΔspmO mutant revealed several biosynthetic precursors en route to SPMs, indicating that key enzymes responsible for the biosynthesis of SPMs should be encoded by genes outside of the identified spm gene cluster. Finally, the biosynthetic pathways of SPM/IDM/LNMs are proposed to lay a basis for further insights into their intriguing biosynthetic machinery.", "gemini": "Các chất đồng phân tryptophan (TD) là một nhóm quan trọng các sản phẩm tự nhiên có hoạt tính sinh học đa dạng và có chung con đường sinh tổng hợp. Chúng tôi báo cáo việc xác định một cụm gen một phần (spm) chịu trách nhiệm cho quá trình sinh tổng hợp một lớp các TD bất thường có bộ khung không phẳng bao gồm spiroindimicin (SPM), indimicin (IDM) và lynamicin (LNM) từ Streptomyces sp. SCSIO 03032 có nguồn gốc từ biển sâu. Phân tích tin sinh học, phá vỡ gen mục tiêu và các nghiên cứu biểu hiện không đồng loại đã xác nhận sự tham gia của cụm gen spm vào quá trình sinh tổng hợp SPM/IDM/LNM và tiết lộ vai trò không thể thiếu của cặp halogenase/reductase SpmHF, amino acid oxidase SpmO và chromopyrrolic acid (CPA) synthase SpmD, cũng như chất điều hòa dương tính SpmR và chất vận chuyển giả định SpmA. Tuy nhiên, cụm gen spm không thể cung cấp cho vật chủ không đồng loại khả năng sản xuất SPM/IDM/LNM. Ngoài ra, enzyme P450 SpmP và monooxygenase SpmX2 được phát hiện không liên quan đến quá trình sinh tổng hợp SPM/IDM/LNM. Căn chỉnh trình tự và mô hình hóa cấu trúc cho thấy thiếu các amino acid bảo tồn chính trong túi liên kết cơ chất của SpmP. Hơn nữa, các thí nghiệm cho ăn ở đột biến ΔspmO không sản xuất đã phát hiện ra một số tiền chất sinh tổng hợp trên đường đến SPM, chỉ ra rằng các enzyme chính chịu trách nhiệm cho quá trình sinh tổng hợp SPM phải được mã hóa bởi các gen nằm ngoài cụm gen spm đã xác định. Cuối cùng, các con đường sinh tổng hợp SPM/IDM/LNM được đề xuất để đặt cơ sở cho những hiểu biết sâu hơn về hệ thống sinh tổng hợp hấp dẫn của chúng."} +{"idx": 2000087, "en": "Microbial polyhydroxyalkanoates (PHA) are a family of biopolyesters with properties similar to petroleum plastics such as polyethylene (PE) or polypropylene (PP). Polyhydroxybutyrate (PHB) is the most common PHA known so far. Clustered regularly interspaced short palindromic repeats interference (CRISPRi), a technology recently developed to control gene expression levels in eukaryotic and prokaryotic genomes, was employed to regulate PHB synthase activity influencing PHB synthesis. Recombinant Escherichia coli harboring an operon of three PHB synthesis genes phaCAB cloned from Ralstonia eutropha, was transformed with various single guided RNA (sgRNA with its guide sequence of 20-23 bases) able to bind to various locations of the PHB synthase PhaC, respectively. Depending on the binding location and the number of sgRNA on phaC, CRISPRi was able to control the phaC transcription and thus PhaC activity. It was found that PHB content, molecular weight, and polydispersity were approximately in direct and reverse proportion to the PhaC activity, respectively. The higher the PhaC activity, the more the intracellular PHB accumulation, yet the less the PHB molecular weights and the wider the polydispersity. This study allowed the PHB contents to be controlled in the ranges of 1.47-75.21% cell dry weights, molecular weights from 2 to 6 millions Dalton and polydispersity of 1.2 to 1.43 in 48 h shake flask studies. This result will be very important for future development of ultrahigh molecular weight PHA useful to meet high strength application requirements.", "gemini": "Polyhydroxyalkanoat vi khuẩn (PHA) là một họ các biopolyeste có các tính chất tương tự như nhựa dầu mỏ như polyetylen (PE) hoặc polypropylen (PP). Polyhydroxybutyrat (PHB) là PHA phổ biến nhất được biết đến cho đến nay. Lặp lại palindromic ngắn xen kẽ có cụm thường xuyên (CRISPRi), một công nghệ gần đây được phát triển để kiểm soát mức độ biểu hiện gen trong bộ gen của eukaryote và prokaryote, đã được sử dụng để điều chỉnh hoạt động của PHB synthase ảnh hưởng đến quá trình tổng hợp PHB. Escherichia coli tái tổ hợp chứa một operon gồm ba gen tổng hợp PHB phaCAB được nhân bản từ Ralstonia eutropha, đã được chuyển đổi bằng nhiều RNA hướng dẫn đơn (sgRNA với trình tự hướng dẫn của nó gồm 20-23 bazơ) có khả năng liên kết với các vị trí khác nhau của PHB synthase PhaC, tương ứng. Tùy thuộc vào vị trí liên kết và số lượng sgRNA trên phaC, CRISPRi có thể kiểm soát phiên mã phaC và do đó là hoạt động của PhaC. Người ta thấy rằng hàm lượng PHB, trọng lượng phân tử và sự đa phân tán tương ứng gần đúng tỷ lệ thuận và tỷ lệ nghịch với hoạt động của PhaC. Hoạt động của PhaC càng cao thì sự tích tụ PHB trong tế bào càng nhiều, nhưng trọng lượng phân tử PHB càng ít và sự đa phân tán càng rộng. Nghiên cứu này cho phép kiểm soát hàm lượng PHB trong phạm vi 1,47-75,21% trọng lượng khô của tế bào, trọng lượng phân tử từ 2 đến 6 triệu Dalton và sự đa phân tán từ 1,2 đến 1,43 trong các nghiên cứu lắc bình 48 giờ. Kết quả này sẽ rất quan trọng cho sự phát triển trong tương lai của PHA có trọng lượng phân tử cực cao hữu ích để đáp ứng các yêu cầu ứng dụng cường độ cao."} +{"idx": 2000088, "en": "Penicillium roqueforti produces several prenylated indole alkaloids, including roquefortine C and clavine alkaloids. The first step in the biosynthesis of roquefortine C is the prenylation of tryptophan-derived dipeptides by a dimethylallyltryptophan synthase, specific for roquefortine biosynthesis (roquefortine prenyltransferase). A second dimethylallyltryptophan synthase, DmaW2, different from the roquefortine prenyltransferase, has been studied in this article. Silencing the gene encoding this second dimethylallyltryptophan synthase, dmaW2, proved that inactivation of this gene does not prevent the production of roquefortine C, but suppresses the formation of other indole alkaloids. Mass spectrometry studies have identified these compounds as isofumigaclavine A, the pathway final product and prenylated intermediates. The silencing does not affect the production of mycophenolic acid and andrastin A. A bioinformatic study of the genome of P. roqueforti revealed that DmaW2 (renamed IfgA) is a prenyltransferase involved in isofumigaclavine A biosynthesis encoded by a gene located in a six genes cluster (cluster A). A second three genes cluster (cluster B) encodes the so-called yellow enzyme and enzymes for the late steps for the conversion of festuclavine to isofumigaclavine A. The yellow enzyme contains a tyrosine-181 at its active center, as occurs in Neosartorya fumigata, but in contrast to the Clavicipitaceae fungi. A complete isofumigaclavines A and B biosynthetic pathway is proposed based on the finding of these studies on the biosynthesis of clavine alkaloids.", "gemini": "Penicillium roqueforti sản xuất một số alkaloid indole được prenyl hóa, bao gồm roquefortine C và alkaloid clavine. Bước đầu tiên trong quá trình sinh tổng hợp roquefortine C là quá trình prenyl hóa các dipeptit có nguồn gốc từ tryptophan của một dimethylallyltryptophan synthase, đặc hiệu cho quá trình sinh tổng hợp roquefortine (roquefortine prenyltransferase). Một dimethylallyltryptophan synthase thứ hai, DmaW2, khác với roquefortine prenyltransferase, đã được nghiên cứu trong bài viết này. Việc làm im lặng gen mã hóa dimethylallyltryptophan synthase thứ hai này, dmaW2, đã chứng minh rằng việc bất hoạt gen này không ngăn cản quá trình sản xuất roquefortine C, nhưng ức chế sự hình thành các alkaloid indole khác. Các nghiên cứu phổ khối đã xác định các hợp chất này là isofumigaclavine A, sản phẩm cuối của con đường và các chất trung gian được prenyl hóa. Sự im lặng không ảnh hưởng đến quá trình sản xuất axit mycophenolic và andrastin A. Một nghiên cứu tin sinh học về bộ gen của P. roqueforti cho thấy DmaW2 (được đổi tên thành IfgA) là một prenyltransferase tham gia vào quá trình sinh tổng hợp isofumigaclavine A được mã hóa bởi một gen nằm trong một cụm sáu gen (cụm A). Một cụm ba gen thứ hai (cụm B) mã hóa cái gọi là enzym vàng và các enzym cho các bước cuối cùng để chuyển đổi festuclavine thành isofumigaclavine A. Enzym vàng chứa tyrosine-181 ở trung tâm hoạt động của nó, như xảy ra ở Neosartorya fumigata, nhưng ngược lại với nấm Clavicipitaceae. Một con đường sinh tổng hợp isofumigaclavines A và B hoàn chỉnh được đề xuất dựa trên phát hiện của các nghiên cứu này về quá trình sinh tổng hợp các alkaloid clavine."} +{"idx": 2000089, "en": "In recent years, there has been a marked increase in the number of approved therapies that increase survival of patients with castration-resistant prostate cancer. Current treatment guidelines provide therapeutic management recommendations, but these are primarily based on clinical factors such as performance status or site of metastasis (bone vs. visceral), and not on underlying molecular or cellular features of disease that may predict response. The ability to tailor treatment based on molecular or cellular features of disease could potentially reduce the occurrence of unnecessary side effects and ineffective treatments, and thereby reduce both direct and indirect medical costs. As such, it is important to identify and validate new prognostic and predictive molecular biomarkers that can be used to direct cancer treatment. This review will focus on existing and potential biomarkers in the context of castration-resistant prostate cancer management and discuss the need for continued discovery and validation of new biomarkers and biomarker panels for prostate cancer.", "gemini": "Trong những năm gần đây, đã có sự gia tăng đáng kể về số lượng các liệu pháp được chấp thuận giúp tăng khả năng sống sót của bệnh nhân ung thư tuyến tiền liệt kháng thiến. Các hướng dẫn điều trị hiện tại cung cấp các khuyến nghị về quản lý điều trị, nhưng chủ yếu dựa trên các yếu tố lâm sàng như tình trạng hoạt động hoặc vị trí di căn (xương so với nội tạng), chứ không dựa trên các đặc điểm phân tử hoặc tế bào cơ bản của bệnh có thể dự đoán được đáp ứng. Khả năng điều chỉnh phương pháp điều trị dựa trên các đặc điểm phân tử hoặc tế bào của bệnh có khả năng làm giảm nguy cơ xảy ra các tác dụng phụ không cần thiết và các phương pháp điều trị không hiệu quả, do đó làm giảm cả chi phí y tế trực tiếp và gián tiếp. Do đó, điều quan trọng là phải xác định và xác nhận các dấu ấn sinh học phân tử tiên lượng và dự đoán mới có thể được sử dụng để chỉ đạo điều trị ung thư. Bài đánh giá này sẽ tập trung vào các dấu ấn sinh học hiện có và tiềm năng trong bối cảnh quản lý ung thư tuyến tiền liệt kháng thiến và thảo luận về nhu cầu tiếp tục phát hiện và xác nhận các dấu ấn sinh học mới và nhóm dấu ấn sinh học cho ung thư tuyến tiền liệt."} +{"idx": 2000090, "en": "Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system (CNS) in young adults that has serious negative socioeconomic effects. In addition to symptoms caused by CNS pathology, the majority of MS patients frequently exhibit gastrointestinal dysfunction, which was previously either explained by the presence of spinal cord lesions or not directly linked to the autoimmune etiology of the disease. Here, we studied the enteric nervous system (ENS) in a B cell- and antibody-dependent mouse model of MS by immunohistochemistry and electron microscopy at different stages of the disease. ENS degeneration was evident prior to the development of CNS lesions and the onset of neurological deficits in mice. The pathology was antibody mediated and caused a significant decrease in gastrointestinal motility, which was associated with ENS gliosis and neuronal loss. We identified autoantibodies against four potential target antigens derived from enteric glia and/or neurons by immunoprecipitation and mass spectrometry. Antibodies against three of the target antigens were also present in the plasma of MS patients as confirmed by ELISA. The analysis of human colon resectates provided evidence of gliosis and ENS degeneration in MS patients compared to non-MS controls. For the first time, this study establishes a pathomechanistic link between the well-established autoimmune attack on the CNS and ENS pathology in MS, which might provide a paradigm shift in our current understanding of the immunopathogenesis of the disease with broad diagnostic and therapeutic implications.", "gemini": "Đa xơ cứng (MS) là một bệnh tự miễn mạn tính của hệ thần kinh trung ương (CNS) ở những người trẻ tuổi có tác động kinh tế xã hội tiêu cực nghiêm trọng. Ngoài các triệu chứng do bệnh lý CNS gây ra, phần lớn bệnh nhân MS thường biểu hiện rối loạn tiêu hóa, trước đây được giải thích là do có tổn thương tủy sống hoặc không liên quan trực tiếp đến nguyên nhân tự miễn của bệnh. Ở đây, chúng tôi đã nghiên cứu hệ thần kinh ruột (ENS) trong mô hình chuột phụ thuộc vào tế bào B và kháng thể của MS bằng phương pháp miễn dịch mô hóa học và kính hiển vi điện tử ở các giai đoạn khác nhau của bệnh. Sự thoái hóa ENS đã rõ ràng trước khi phát triển các tổn thương CNS và khởi phát các khiếm khuyết về thần kinh ở chuột. Bệnh lý học được trung gian bởi kháng thể và gây ra tình trạng giảm đáng kể nhu động đường tiêu hóa, liên quan đến chứng loạn dưỡng thần kinh ENS và mất tế bào thần kinh. Chúng tôi đã xác định các tự kháng thể chống lại bốn kháng nguyên đích tiềm năng có nguồn gốc từ tế bào thần kinh đệm ruột và/hoặc tế bào thần kinh bằng phương pháp kết tủa miễn dịch và phổ khối. Kháng thể chống lại ba kháng nguyên đích cũng có trong huyết tương của bệnh nhân MS như đã xác nhận bằng phương pháp ELISA. Phân tích các mẫu cắt bỏ đại tràng của người đã cung cấp bằng chứng về chứng loạn dưỡng thần kinh và thoái hóa ENS ở bệnh nhân MS so với những người đối chứng không mắc MS. Lần đầu tiên, nghiên cứu này thiết lập mối liên hệ về cơ chế bệnh sinh giữa tình trạng tấn công tự miễn đã được xác định rõ trên CNS và bệnh lý ENS ở MS, điều này có thể tạo ra sự thay đổi mô hình trong hiểu biết hiện tại của chúng ta về sự sinh bệnh miễn dịch của bệnh với những tác động rộng rãi về chẩn đoán và điều trị."} +{"idx": 2000091, "en": "The kinetics of reactions of cobalamin (II) and cobinamide (II) with sulfur dioxide was studied by UV-visible (UV-vis) spectroscopy. Reaction results in oxidation of Co(II) center and involves two aquated SO2 moieties. The final product is suggested to be complex Co(III)-S2O 4•- . The absence of corrin ring modifications during the reactions was proved.", "gemini": "Động học của phản ứng của cobalamin (II) và cobinamid (II) với lưu huỳnh đioxit đã được nghiên cứu bằng phương pháp quang phổ UV-nhìn thấy (UV-vis). Kết quả phản ứng trong quá trình oxy hóa trung tâm Co(II) và liên quan đến hai nhóm SO2 ngậm nước. Sản phẩm cuối cùng được cho là phức hợp Co(III)-S2O 4•-. Sự vắng mặt của các sửa đổi vòng corrin trong quá trình phản ứng đã được chứng minh."} +{"idx": 2000092, "en": "Calcium (Ca2+) plays essential roles in generative reproduction of angiosperms, but the sites and mechanisms of Ca2+ storage and mobilization during pollen-pistil interactions have not been fully defined. Both external and internal Ca2+ stores are likely important during male gametophyte communication with the sporophytic and gametophytic cells within the pistil. Given that calreticulin (CRT), a Ca2+-buffering protein, is able to bind Ca2+ reversibly, it can serve as a mobile store of easily releasable Ca2+ (so called an exchangeable Ca2+) in eukaryotic cells. CRT has typical endoplasmic reticulum (ER) targeting and retention signals and resides primarily in the ER. However, localization of this protein outside the ER has also been revealed in both animal and plant cells, including Golgi/dictyosomes, nucleus, plasma membrane/cell surface, plasmodesmata, and even extracellular matrix. These findings indicate that CRT may function in a variety of different cell compartments and specialized structures. We have recently shown that CRT is highly expressed and accumulated in the ER of plant cells involved in pollen-pistil interactions in Petunia, and we proposed an essential role for CRT in intracellular Ca2+ storage and mobilization during the key reproductive events. Here, we demonstrate that both CRT and exchangeable Ca2+ are localized in the intra/extracellular peripheries of highly specialized plant cells, such as the pistil transmitting tract cells, pollen tubes, nucellus cells surrounding the embryo sac, and synergids. Based on our present results, we propose that extracellularly located CRT is also involved in Ca2+ storage and mobilization during sexual reproduction of angiosperms.", "gemini": "Canxi (Ca2+) đóng vai trò thiết yếu trong quá trình sinh sản hữu tính của thực vật hạt kín, nhưng các vị trí và cơ chế lưu trữ và huy động Ca2+ trong quá trình tương tác giữa phấn hoa và nhụy vẫn chưa được xác định đầy đủ. Cả kho dự trữ Ca2+ bên ngoài và bên trong đều có thể quan trọng trong quá trình giao tiếp của giao tử đực với các tế bào giao tử và bào tử trong nhụy. Do calreticulin (CRT), một protein đệm Ca2+, có khả năng liên kết thuận nghịch với Ca2+, nên nó có thể đóng vai trò như một kho dự trữ di động của Ca2+ dễ giải phóng (còn gọi là Ca2+ có thể trao đổi) trong các tế bào nhân chuẩn. CRT có các tín hiệu nhắm mục tiêu và giữ lại lưới nội chất điển hình (ER) và chủ yếu nằm trong ER. Tuy nhiên, vị trí của protein này bên ngoài ER cũng đã được phát hiện ở cả tế bào động vật và thực vật, bao gồm Golgi/dictyosome, nhân, màng tế bào/bề mặt tế bào, plasmodesmata và thậm chí cả ma trận ngoại bào. Những phát hiện này chỉ ra rằng CRT có thể hoạt động trong nhiều ngăn tế bào và cấu trúc chuyên biệt khác nhau. Gần đây, chúng tôi đã chứng minh rằng CRT được biểu hiện và tích tụ nhiều trong ER của các tế bào thực vật tham gia vào quá trình tương tác giữa phấn hoa và nhụy ở cây Petunia, và chúng tôi đề xuất một vai trò thiết yếu của CRT trong quá trình lưu trữ và huy động Ca2+ trong tế bào trong các sự kiện sinh sản chính. Ở đây, chúng tôi chứng minh rằng cả CRT và Ca2+ có thể trao đổi đều nằm ở ngoại biên trong/ngoài tế bào của các tế bào thực vật chuyên biệt cao, chẳng hạn như các tế bào đường dẫn truyền nhụy, ống phấn, tế bào nucellus xung quanh túi phôi và tế bào hiệp đồng. Dựa trên kết quả hiện tại của chúng tôi, chúng tôi đề xuất rằng CRT nằm ngoài tế bào cũng tham gia vào quá trình lưu trữ và huy động Ca2+ trong quá trình sinh sản hữu tính của thực vật hạt kín."} +{"idx": 2000093, "en": "The impact of selected metallophthalocyanines, featuring diverse molecular structure, upon the fluidity of liposome membranes was studied using the spin label EPR technique. The \"mono\"-type MPc's (M = Zn, Sn; Pc = C32H16N8 is the phthalocyanine ligand) and sandwich LnPc2 complexes (Ln = Nd, Sm, Gd) were explored. Liposomes were obtained in a sonication process, from egg yolk lecithin (EYL) in water. TEMPO and 16-DOXYL spin labels were used to monitor the peripheral and central part of the lipid double layer, respectively, which allowed to localize the phthalocyanine additive within the bilayer, as well as to perform independent measurements of changes in fluidity upon addition thereof. All the complexes tested were found to increase the fluidity in the middle of the lipid bilayer. However, at the water-lipid interface the LnPc2 compounds showed a relative small effect upon the phospholipids' arrangement, whereas in the case of ZnPc and SnPc it was found much more pronounced. EPR results were supplemented by measurements of static electrical charge, the investigated phthalocyanines may potentially feed into the membrane thus affecting its stability.", "gemini": "Tác động của các metallophthalocyanine đã chọn, có cấu trúc phân tử đa dạng, lên tính lưu động của màng liposome đã được nghiên cứu bằng kỹ thuật EPR nhãn spin. Các MPc loại \"mono\" (M = Zn, Sn; Pc = C32H16N8 là phối tử phthalocyanine) và các phức hợp sandwich LnPc2 (Ln = Nd, Sm, Gd) đã được khám phá. Liposome thu được trong quá trình siêu âm, từ lecithin lòng đỏ trứng (EYL) trong nước. Nhãn spin TEMPO và 16-DOXYL được sử dụng để theo dõi phần ngoại vi và phần trung tâm của lớp đôi lipid, cho phép xác định vị trí của chất phụ gia phthalocyanine trong lớp đôi, cũng như thực hiện các phép đo độc lập về những thay đổi về tính lưu động sau khi bổ sung chất đó. Tất cả các phức hợp được thử nghiệm đều làm tăng tính lưu động ở giữa lớp đôi lipid. Tuy nhiên, ở giao diện nước-lipid, các hợp chất LnPc2 cho thấy tác động tương đối nhỏ lên sự sắp xếp của phospholipid, trong khi trong trường hợp ZnPc và SnPc, tác động này được thấy rõ hơn nhiều. Kết quả EPR được bổ sung bằng các phép đo điện tích tĩnh, các phthalocyanine được nghiên cứu có khả năng đi vào màng do đó ảnh hưởng đến độ ổn định của màng."} +{"idx": 2000094, "en": "The importance of the dipeptidyl peptidase 4 (DPP4) gene family in regulating critical biochemical pathways continues to emerge. The two most well-studied members of the family, DPP4 and fibroblast activation protein (FAP), have been investigated both as therapeutic targets for disease and as diagnostic biomarkers. The interest in DPP4 and FAP as potential disease biomarkers has been driven primarily by observations of altered expression profiles in inflammatory diseases and cancer. Furthermore, the stability and persistence of soluble DPP4 and FAP in the serum make them attractive candidate serology markers. This review summarises investigations into DPP4 and FAP as biomarkers of autoimmune disease, gut inflammation, psychosomatic disorders and malignancy and discusses their potential likelihood as clinically useful tools.", "gemini": "Tầm quan trọng của họ gen dipeptidyl peptidase 4 (DPP4) trong việc điều hòa các con đường sinh hóa quan trọng vẫn tiếp tục xuất hiện. Hai thành viên được nghiên cứu tốt nhất của họ gen này, DPP4 và protein hoạt hóa nguyên bào sợi (FAP), đã được nghiên cứu như là các mục tiêu điều trị bệnh và là các dấu ấn sinh học chẩn đoán. Sự quan tâm đến DPP4 và FAP như các dấu ấn sinh học bệnh tiềm năng chủ yếu được thúc đẩy bởi các quan sát về hồ sơ biểu hiện bị thay đổi trong các bệnh viêm và ung thư. Hơn nữa, độ ổn định và tính bền vững của DPP4 hòa tan và FAP trong huyết thanh khiến chúng trở thành các dấu ấn huyết thanh ứng cử viên hấp dẫn. Tổng quan này tóm tắt các cuộc điều tra về DPP4 và FAP như các dấu ấn sinh học của bệnh tự miễn, viêm ruột, rối loạn tâm lý và ác tính và thảo luận về khả năng tiềm ẩn của chúng như các công cụ hữu ích về mặt lâm sàng."} +{"idx": 2000095, "en": "Artificial lipid bilayers have been extensively studied as models that mimic natural membranes (biomimetic membranes). Several attempts of biomimetic membranes inserting ubiquinone (UQ) have been performed to enlighten which the position of UQ in the lipid layer is, although obtaining contradictory results. In this work, pure components (DPPC and UQ) and DPPC:UQ mixtures have been studied using surface pressure-area isotherms and Langmuir-Blodgett (LB) films of the same compounds have been transferred onto solid substrates being topographically characterized on mica using atomic force microscopy and electrochemically on indium tin oxide slides. DPPC:UQ mixtures present less solid-like physical state than pure DPPC indicating a higher-order degree for the latter. UQ influences considerably DPPC during the fluid state, but it is mainly expelled after the phase transition at [Formula: see text] 26 mN·m^-1 for the 5:1 ratio and at [Formula: see text] 21 mN·m^-1 for lower UQ content. The thermodynamic studies confirm the stability of the DPPC:UQ mixtures before that event, although presenting a non-ideal behaviour. The results indicate that UQ position can be tuned by means of the surface pressure applied to obtain LB films and the UQ initial content. The UQ positions in the biomimetic membrane are distinguished by their formal potential: UQ located in \"diving\" position with the UQ placed in the DPPC matrix in direct contact with the electrode surface ( -0.04±0.02 V), inserted between lipid chains without contact to the substrate ( 0.00±0.01 V) and parallel to the substrate, above the lipid chains ( 0.09±0.02 V).", "gemini": "Các lớp đôi lipid nhân tạo đã được nghiên cứu rộng rãi như các mô hình bắt chước màng tự nhiên (màng sinh học). Một số nỗ lực của màng sinh học chèn ubiquinone (UQ) đã được thực hiện để làm sáng tỏ vị trí của UQ trong lớp lipid, mặc dù thu được kết quả trái ngược nhau. Trong công trình này, các thành phần tinh khiết (DPPC và UQ) và hỗn hợp DPPC:UQ đã được nghiên cứu bằng cách sử dụng các đường đẳng nhiệt diện tích áp suất bề mặt và các màng Langmuir-Blodgett (LB) của các hợp chất tương tự đã được chuyển lên các chất nền rắn được đặc trưng về mặt địa hình trên mica bằng kính hiển vi lực nguyên tử và điện hóa trên các phiến oxit thiếc indium. Các hỗn hợp DPPC:UQ có trạng thái vật lý giống chất rắn ít hơn DPPC tinh khiết, cho thấy cấp bậc cao hơn đối với chất sau. UQ ảnh hưởng đáng kể đến DPPC trong trạng thái lỏng, nhưng chủ yếu bị đẩy ra sau quá trình chuyển pha ở [Công thức: xem văn bản] 26 mN·m^-1 đối với tỷ lệ 5:1 và ở [Công thức: xem văn bản] 21 mN·m^-1 đối với hàm lượng UQ thấp hơn. Các nghiên cứu nhiệt động lực học xác nhận tính ổn định của hỗn hợp DPPC:UQ trước sự kiện đó, mặc dù biểu hiện hành vi không lý tưởng. Kết quả cho thấy vị trí UQ có thể được điều chỉnh bằng áp suất bề mặt được áp dụng để thu được màng LB và hàm lượng UQ ban đầu. Các vị trí UQ trong màng sinh học được phân biệt bằng thế thức của chúng: UQ nằm ở vị trí \"lặn\" với UQ được đặt trong ma trận DPPC tiếp xúc trực tiếp với bề mặt điện cực ( -0,04±0,02 V), được chèn giữa các chuỗi lipid mà không tiếp xúc với chất nền ( 0,00±0,01 V) và song song với chất nền, phía trên các chuỗi lipid ( 0,09±0,02 V)."} +{"idx": 2000096, "en": "Ferredoxins are soluble iron sulphur proteins which function as electron donors in a number of metabolic pathways in a broad range of organisms. In photosynthetic organisms, PETF, or ferredoxin 1 (FDX1), is the most studied ferredoxin due to its essential role in photosynthesis, where it transfers electrons from photosystem I to ferredoxin-NADP+ oxidoreductase. However, PETF can also transfer electrons to a large number of other proteins. One important PETF electron acceptor found in green microalgae is the biologically and biotechnologically important [FeFe]-hydrogenase HYDA, which catalyses the production of molecular hydrogen (H2) from protons and electrons. The interaction between PETF and HYDA is of considerable interest, as PETF is the primary electron donor to HYDA and electron supply is one of the main limiting factors for H2 production on a commercial scale. Although there is no three dimensional structure of the PETF-HYDA complex available, protein variants, nuclear magnetic resonance titration studies, molecular dynamics and modelling have provided considerable insight into the residues essential for forming and maintaining the interaction. In this review, we discuss the most recent findings with regard to ferredoxin-HYDA interactions and the evolution of the various Chlamydomonas reinhardtii ferredoxin isoforms. Finally, we provide an outlook on new PETF-based biotechnological approaches for improved H2 production efficiencies.", "gemini": "Ferredoxin là các protein sắt lưu huỳnh hòa tan có chức năng như các chất cho điện tử trong một số con đường chuyển hóa ở nhiều loại sinh vật. Ở các sinh vật quang hợp, PETF hoặc ferredoxin 1 (FDX1) là ferredoxin được nghiên cứu nhiều nhất do vai trò thiết yếu của nó trong quá trình quang hợp, nơi nó truyền các điện tử từ hệ thống quang hợp I sang ferredoxin-NADP+ oxidoreductase. Tuy nhiên, PETF cũng có thể truyền các điện tử đến rất nhiều protein khác. Một thụ thể điện tử PETF quan trọng được tìm thấy trong tảo lục là [FeFe] -hydrogenase HYDA có tầm quan trọng về mặt sinh học và công nghệ sinh học, có tác dụng xúc tác quá trình sản xuất hydro phân tử (H2) từ các proton và điện tử. Sự tương tác giữa PETF và HYDA rất được quan tâm, vì PETF là chất cho điện tử chính cho HYDA và nguồn cung cấp điện tử là một trong những yếu tố chính hạn chế sản xuất H2 ở quy mô thương mại. Mặc dù không có cấu trúc ba chiều nào của phức hợp PETF-HYDA, các biến thể protein, các nghiên cứu chuẩn độ cộng hưởng từ hạt nhân, động học phân tử và mô hình hóa đã cung cấp cái nhìn sâu sắc đáng kể về các chất còn lại cần thiết để hình thành và duy trì sự tương tác. Trong bài đánh giá này, chúng tôi thảo luận về những phát hiện mới nhất liên quan đến tương tác ferredoxin-HYDA và sự tiến hóa của các dạng đồng phân ferredoxin Chlamydomonas reinhardtii khác nhau. Cuối cùng, chúng tôi đưa ra triển vọng về các phương pháp tiếp cận công nghệ sinh học mới dựa trên PETF để cải thiện hiệu quả sản xuất H2."} +{"idx": 2000097, "en": "PURPOSE Physical activity can enhance quality of life in cancer survivors, but this conclusion is based largely on research linking moderate-to-vigorous physical activity with quality of life. Light-intensity physical activity may be more feasible than more strenuous exercise for many older cancer survivors. This study reports a secondary analysis of baseline data from a lifestyle behavior intervention trial and examines the hypothesis that older cancer survivors who engage in more light-intensity physical activity, independent of moderate-to-vigorous activity, will report better mental quality of life. METHODS Older (≥65 years), overweight or obese breast, prostate, or colorectal cancer survivors (n = 641, 54% female) self-reported their physical activity and mental quality of life (i.e., mental health, emotional role functioning, vitality, and social role functioning from the Short-Form 36 Health Status Survey) as a part of the RENEW trial baseline assessment. Analysis of covariance was used to test hypotheses. RESULTS For older women (but not men), light physical activity was positively associated with mental quality of life after adjusting for moderate-to-vigorous physical activity. Light physical activity that involved social participation appeared to be responsible for this association. For older men (but not women), moderate-to-vigorous physical activity was positively associated with mental quality of life. CONCLUSIONS Some activity appears to be better than none for important dimensions of mental quality of life. Experimental research is needed to test the hypothesis that older cancer survivors should strive to avoid inactivity regardless of whether they are able to engage in moderate-to-vigorous physical activity.", "gemini": "MỤC ĐÍCH Hoạt động thể chất có thể nâng cao chất lượng cuộc sống cho những người sống sót sau ung thư, nhưng kết luận này chủ yếu dựa trên nghiên cứu liên kết hoạt động thể chất vừa phải đến mạnh mẽ với chất lượng cuộc sống. Hoạt động thể chất cường độ nhẹ có thể khả thi hơn so với các bài tập gắng sức hơn đối với nhiều người cao tuổi sống sót sau ung thư. Nghiên cứu này báo cáo một phân tích thứ cấp về dữ liệu ban đầu từ một thử nghiệm can thiệp hành vi lối sống và kiểm tra giả thuyết rằng những người cao tuổi sống sót sau ung thư tham gia nhiều hơn vào hoạt động thể chất cường độ nhẹ, không phụ thuộc vào hoạt động vừa phải đến mạnh mẽ, sẽ báo cáo chất lượng cuộc sống về mặt tinh thần tốt hơn. PHƯƠNG PHÁP Những người cao tuổi (≥65 tuổi), thừa cân hoặc béo phì, ung thư vú, tuyến tiền liệt hoặc trực tràng (n = 641, 54% nữ) tự báo cáo hoạt động thể chất và chất lượng cuộc sống về mặt tinh thần của họ (tức là sức khỏe tâm thần, chức năng vai trò tình cảm, sức sống và chức năng vai trò xã hội từ Khảo sát tình trạng sức khỏe ngắn gọn 36) như một phần của đánh giá cơ sở thử nghiệm RENEW. Phân tích hiệp phương sai được sử dụng để kiểm tra các giả thuyết. KẾT QUẢ Đối với phụ nữ lớn tuổi (nhưng không phải nam giới), hoạt động thể chất nhẹ có liên quan tích cực đến chất lượng cuộc sống về mặt tinh thần sau khi điều chỉnh hoạt động thể chất vừa phải đến mạnh mẽ. Hoạt động thể chất nhẹ liên quan đến sự tham gia xã hội dường như chịu trách nhiệm cho mối liên hệ này. Đối với nam giới lớn tuổi (nhưng không phải nữ giới), hoạt động thể chất vừa phải đến mạnh mẽ có liên quan tích cực đến chất lượng cuộc sống về mặt tinh thần. KẾT LUẬN Một số hoạt động dường như tốt hơn không đối với các chiều hướng quan trọng của chất lượng cuộc sống về mặt tinh thần. Cần có nghiên cứu thực nghiệm để kiểm tra giả thuyết rằng những người cao tuổi sống sót sau ung thư nên cố gắng tránh tình trạng không hoạt động bất kể họ có khả năng tham gia hoạt động thể chất vừa phải đến mạnh mẽ hay không."} +{"idx": 2000098, "en": "Physical activity is emerging from epidemiologic research as a lifestyle factor that may improve survival from colorectal, breast, and prostate cancers. However, there is considerably less evidence relating physical activity to cancer recurrence and the biologic mechanisms underlying this association remain unclear. Cancer patients are surviving longer than ever before, and fear of cancer recurrence is an important concern. Herein, we provide an overview of the current epidemiologic evidence relating physical activity to cancer recurrence. We review the biologic mechanisms most commonly researched in the context of physical activity and cancer outcomes, and, using the example of colorectal cancer, we explore hypothesized mechanisms through which physical activity might intervene in the colorectal recurrence pathway. Our review highlights the importance of considering pre-diagnosis and post-diagnosis activity, as well as cancer stage and timing of recurrence, in epidemiologic studies. In addition, more epidemiologic research is needed with cancer recurrence as a consistently defined outcome studied separately from survival. Future mechanistic research using randomized controlled trials, specifically those demonstrating the exercise responsiveness of hypothesized mechanisms in early stages of carcinogenesis, are needed to inform recommendations about when to exercise and to anticipate additive or synergistic effects with other preventive behaviors or treatments.", "gemini": "Hoạt động thể chất đang nổi lên từ nghiên cứu dịch tễ học như một yếu tố lối sống có thể cải thiện tỷ lệ sống sót sau ung thư đại trực tràng, ung thư vú và ung thư tuyến tiền liệt. Tuy nhiên, có ít bằng chứng liên quan đến hoạt động thể chất với tái phát ung thư và các cơ chế sinh học cơ bản của mối liên hệ này vẫn chưa rõ ràng. Bệnh nhân ung thư đang sống lâu hơn bao giờ hết và nỗi sợ ung thư tái phát là một mối quan tâm quan trọng. Trong bài viết này, chúng tôi cung cấp một cái nhìn tổng quan về các bằng chứng dịch tễ học hiện tại liên quan đến hoạt động thể chất với tái phát ung thư. Chúng tôi xem xét các cơ chế sinh học được nghiên cứu phổ biến nhất trong bối cảnh hoạt động thể chất và kết quả ung thư, và sử dụng ví dụ về ung thư đại trực tràng, chúng tôi khám phá các cơ chế được giả định thông qua đó hoạt động thể chất có thể can thiệp vào quá trình tái phát ung thư đại trực tràng. Đánh giá của chúng tôi nhấn mạnh tầm quan trọng của việc xem xét hoạt động trước chẩn đoán và sau chẩn đoán, cũng như giai đoạn ung thư và thời điểm tái phát, trong các nghiên cứu dịch tễ học. Ngoài ra, cần có thêm nghiên cứu dịch tễ học với tái phát ung thư như một kết cục được xác định nhất quán, được nghiên cứu riêng biệt với tỷ lệ sống sót. Nghiên cứu cơ chế trong tương lai sử dụng các thử nghiệm có đối chứng ngẫu nhiên, đặc biệt là những thử nghiệm chứng minh khả năng đáp ứng với bài tập của các cơ chế được giả định trong giai đoạn đầu của quá trình gây ung thư, là cần thiết để đưa ra các khuyến nghị về thời điểm tập thể dục và dự đoán các tác động cộng gộp hoặc hiệp đồng với các hành vi hoặc phương pháp điều trị dự phòng khác."} +{"idx": 2000099, "en": "PURPOSE To compare intravitreal ranibizumab and dexamethasone implant in patients with macular edema (ME) secondary to central retinal vein occlusion (CRVO). METHODS Participants were 42 treatment naive patients with ME due to CRVO, who received either intravitreal 0.5 mg ranibizumab (n = 25) or intravitreal 0.7 mg dexamethasone implant (n = 17). The main outcomes included the mean change in best corrected visual acuity (BCVA) and central subfield thickness (CST) at month 12 compared to baseline in the two groups. RESULTS At month 12, there was no statistically significant difference in BCVA and CST change between the two groups. However, there was recurrence in ME at month 5 in the dexamethasone group. CONCLUSIONS Both ranibizumab and dexamethasone implant were found to be safe and effective at the 12-month follow-up in patients with ME secondary to CRVO. Since there was a recurrence in ME at month 5 in the dexamethasone group, we suggested that intravitreal injection of dexamethasone implant should be potentially administered sooner than 6 months.", "gemini": "MỤC ĐÍCH So sánh thuốc cấy ghép ranibizumab và dexamethasone nội nhãn ở những bệnh nhân bị phù hoàng điểm (ME) thứ phát do tắc tĩnh mạch trung tâm võng mạc (CRVO). PHƯƠNG PHÁP Người tham gia là 42 bệnh nhân chưa từng điều trị bị ME do CRVO, những người đã nhận được thuốc ranibizumab 0,5 mg nội nhãn (n = 25) hoặc thuốc cấy ghép dexamethasone 0,7 mg nội nhãn (n = 17). Các kết quả chính bao gồm sự thay đổi trung bình về thị lực được hiệu chỉnh tốt nhất (BCVA) và độ dày dưới trường trung tâm (CST) ở tháng thứ 12 so với giá trị ban đầu ở hai nhóm. KẾT QUẢ Vào tháng thứ 12, không có sự khác biệt có ý nghĩa thống kê về thay đổi BCVA và CST giữa hai nhóm. Tuy nhiên, có sự tái phát ME ở tháng thứ 5 trong nhóm dexamethasone. KẾT LUẬN Cả thuốc cấy ghép ranibizumab và dexamethasone đều được thấy là an toàn và hiệu quả trong thời gian theo dõi 12 tháng ở những bệnh nhân bị ME thứ phát do CRVO. Vì có sự tái phát ME ở tháng thứ 5 trong nhóm dexamethasone, chúng tôi đề xuất rằng nên tiêm thuốc cấy ghép dexamethasone nội nhãn sớm hơn 6 tháng."} +{"idx": 2000100, "en": "Chronic cerebral hypoperfusion (CCH) evokes mild cognitive impairment (MCI) and contributes to the progression of vascular dementia and Alzheimer's disease (AD). How CCH induces these neurodegenerative processes that may spread along the synaptic network and whether they are detectable at the synaptic proteome level of the cerebral cortex remains to be established. In the present study, we report the synaptic protein changes in the cerebral cortex after stepwise bilateral common carotid artery occlusion (BCCAO) induced CCH in the rat. The occlusions were confirmed with magnetic resonance angiography 5 weeks after the surgery. Synaptosome fractions were prepared using sucrose gradient centrifugation from cerebral cortex dissected 7 weeks after the occlusion. The synaptic protein differences between the sham operated and CCH groups were analyzed with label-free nanoUHPLC-MS/MS. We identified 46 proteins showing altered abundance due to CCH. In particular, synaptic protein and lipid metabolism, as well as GABA shunt-related proteins showed increased while neurotransmission and synaptic assembly-related proteins showed decreased protein level changes in CCH rats. Protein network analysis of CCH-induced protein alterations suggested the importance of increased synaptic apolipoprotein E (APOE) level as a consequence of CCH. Therefore, the change in APOE level was confirmed with Western blotting. The identified synaptic protein changes would precede the onset of dementia-like symptoms in the CCH model, suggesting their importance in the development of vascular dementia.", "gemini": "Giảm tưới máu não mạn tính (CCH) gây ra suy giảm nhận thức nhẹ (MCI) và góp phần vào sự tiến triển của chứng mất trí mạch máu và bệnh Alzheimer (AD). Vẫn chưa xác định được cách CCH gây ra các quá trình thoái hóa thần kinh này có thể lan truyền dọc theo mạng lưới synap hay liệu chúng có thể phát hiện được ở cấp độ proteome synap của vỏ não hay không. Trong nghiên cứu hiện tại, chúng tôi báo cáo những thay đổi về protein synap ở vỏ não sau khi tắc động mạch cảnh chung hai bên từng bước (BCCAO) gây ra CCH ở chuột. Việc tắc nghẽn đã được xác nhận bằng chụp động mạch cộng hưởng từ 5 tuần sau phẫu thuật. Các phân đoạn synaptosome được chuẩn bị bằng cách ly tâm gradien sucrose từ vỏ não được mổ 7 tuần sau khi tắc nghẽn. Sự khác biệt về protein synap giữa các nhóm giả phẫu và CCH đã được phân tích bằng nanoUHPLC-MS / MS không có nhãn. Chúng tôi đã xác định được 46 protein cho thấy sự thay đổi về số lượng do CCH. Đặc biệt, protein synap và chuyển hóa lipid, cũng như các protein liên quan đến đường phân lưu GABA cho thấy sự gia tăng trong khi các protein liên quan đến truyền thần kinh và lắp ráp synap cho thấy sự thay đổi mức protein giảm ở chuột CCH. Phân tích mạng lưới protein của các biến đổi protein do CCH gây ra cho thấy tầm quan trọng của việc tăng mức apolipoprotein E (APOE) synap như một hậu quả của CCH. Do đó, sự thay đổi về mức APOE đã được xác nhận bằng phương pháp Western blotting. Những thay đổi về protein synap được xác định sẽ đi trước sự khởi phát của các triệu chứng giống như chứng mất trí trong mô hình CCH, cho thấy tầm quan trọng của chúng trong sự phát triển của chứng mất trí mạch máu."} +{"idx": 2000101, "en": "PURPOSE To report the results of long-term topical treatment with bevacizumab (Avastin) 5 mg/mL eyedrops in a case of aniridia-related neovacularization of the cornea. METHODS Interventional case report. A female patient with aniridia had a decrease in the best corrected visual acuity from 0.32 to 0.02 in the OS over the course of 4 years, secondary to central corneal neovascularization and epithelial breakdown. Vision in the OD was 0.2. In 2008, at age 28, a shared decision was made to start off-label treatment with bevacizumab eyedrops 0.5% in both eyes. After 9 years, the visual acuity in the OD remained stable, with stability of the macropannus and maintenance of central corneal clarity. In the OS, the central corneal neovascularization regressed, the epithelium regained its clarity, and after cataract surgery visual acuity was regained to 0.32. RESULTS After 9 years of treatment with topical bevacizumab, vision acuity is comparable to the situation of 12 years previously: Visual acuity remained stable in the ODS. CONCLUSION In a young patient with progressive corneal neovascularization secondary to aniridia, stability of central corneal neovascularization was obtained and corneal clarity was preserved by adding a daily drop of bevacizumab 5 mg/mL. No adverse events occurred. Vessel growth was inhibited, and as such, the progression of the natural history of the patient's disease was halted. More clinical study with longer follow-up is needed to investigate the applicability of treatment with topical VEGF inhibitors in aniridia.", "gemini": "MỤC ĐÍCH Báo cáo kết quả điều trị tại chỗ dài hạn bằng thuốc nhỏ mắt bevacizumab (Avastin) 5 mg/mL trong một trường hợp tân mạch hóa giác mạc liên quan đến chứng aniridia. PHƯƠNG PHÁP Báo cáo trường hợp can thiệp. Một bệnh nhân nữ bị aniridia đã giảm thị lực được hiệu chỉnh tốt nhất từ 0,32 xuống 0,02 ở OS trong quá trình 4 năm, do tân mạch hóa giác mạc trung tâm và biểu mô bị phá vỡ. Thị lực ở OD là 0,2. Năm 2008, ở tuổi 28, một quyết định chung đã được đưa ra để bắt đầu điều trị ngoại nhãn bằng thuốc nhỏ mắt bevacizumab 0,5% ở cả hai mắt. Sau 9 năm, thị lực ở OD vẫn ổn định, với sự ổn định của macropannus và duy trì độ trong của giác mạc trung tâm. Ở OS, tân mạch hóa giác mạc trung tâm đã thoái triển, biểu mô lấy lại độ trong và sau phẫu thuật đục thủy tinh thể, thị lực đã phục hồi đến 0,32. KẾT QUẢ Sau 9 năm điều trị bằng bevacizumab tại chỗ, thị lực tương đương với tình trạng của 12 năm trước: Thị lực vẫn ổn định ở ODS. KẾT LUẬN Ở một bệnh nhân trẻ bị tân mạch hóa giác mạc tiến triển thứ phát do chứng aniridia, sự ổn định của tân mạch hóa giác mạc trung tâm đã đạt được và độ trong của giác mạc được bảo tồn bằng cách nhỏ một giọt bevacizumab 5 mg/mL hàng ngày. Không có tác dụng phụ nào xảy ra. Sự phát triển của mạch máu đã bị ức chế và do đó, sự tiến triển của quá trình bệnh tự nhiên của bệnh nhân đã dừng lại. Cần có nhiều nghiên cứu lâm sàng hơn với thời gian theo dõi dài hơn để điều tra khả năng áp dụng của phương pháp điều trị bằng chất ức chế VEGF tại chỗ trong chứng aniridia."} +{"idx": 2000102, "en": "PURPOSE To provide preliminary data about efficacy and safety of plasma rich in growth factors (PRGF) eye-drops in neurotrophic keratitis (NK) and to analyze the possible influence of certain variables on treatment outcomes. METHODS This retrospective study included patients with stages 2-3 of NK treated with PRGF eye-drops. Primary endpoint was the resolution time of corneal ulcer defect. Outcome measures including percentage of ulcer closure at 4 weeks, Ocular Surface Disease Index (OSDI), Best-Corrected Visual Acuity (BCVA) and Visual Analogue Scale (VAS) were also evaluated before and after treatment with PRGF. The influence of some patients' clinical variables on results was assessed. Safety assessment was also performed reporting all adverse events. RESULTS Thirty-eight treated eyes in a total of thirty-one patients were evaluated, of which five cases had no prior response to autologous serum treatment. Most cases (97.4%) achieved the complete resolution of corneal defect/ulcer. Mean resolution time was 11.4 weeks (SD = 13.7). A statistical significant (p < 0.05) reduction in OSDI (60.9%), VAS frequency (59.9%), VAS severity (57.0%) and improvement in BCVA (52.8%) was observed. The results were stratified according to the pathology stage and to the identified potential effect modifiers variables. Only one adverse event was reported in one patient (2.6%). CONCLUSIONS PRGF eye-drops could be a safe and effective therapeutic option for patients with stages 2-3 of NK, showing high rates of corneal defect/ulcer resolution in short times, either in reducing signs and symptoms of NK, and therefore preventing the progression of NK to greater ocular complications.", "gemini": "MỤC ĐÍCH Cung cấp dữ liệu sơ bộ về hiệu quả và độ an toàn của thuốc nhỏ mắt giàu huyết tương có yếu tố tăng trưởng (PRGF) trong viêm giác thần kinh (NK) và phân tích tác động có thể có của một số biến số nhất định đến kết quả điều trị. PHƯƠNG PHÁP Nghiên cứu hồi cứu này bao gồm những bệnh nhân bị NK giai đoạn 2-3 được điều trị bằng thuốc nhỏ mắt PRGF. Điểm cuối chính là thời gian phục hồi khiếm khuyết loét giác mạc. Các biện pháp kết quả bao gồm tỷ lệ phần trăm liền loét ở tuần thứ 4, Chỉ số bệnh bề mặt nhãn cầu (OSDI), Độ sắc nét thị lực được hiệu chỉnh tốt nhất (BCVA) và Thang đo tương tự thị giác (VAS) cũng được đánh giá trước và sau khi điều trị bằng PRGF. Ảnh hưởng của một số biến số lâm sàng của bệnh nhân đến kết quả đã được đánh giá. Đánh giá độ an toàn cũng được thực hiện bằng cách báo cáo tất cả các tác dụng phụ. KẾT QUẢ Đã đánh giá ba mươi tám mắt được điều trị ở tổng số ba mươi mốt bệnh nhân, trong đó năm trường hợp trước đó không có đáp ứng với phương pháp điều trị huyết thanh tự thân. Hầu hết các trường hợp (97,4%) đạt được độ phục hồi hoàn toàn khiếm khuyết/loét giác mạc. Thời gian phục hồi trung bình là 11,4 tuần (SD = 13,7). Đã quan sát thấy sự giảm đáng kể về mặt thống kê (p < 0,05) trong OSDI (60,9%), tần suất VAS (59,9%), mức độ nghiêm trọng của VAS (57,0%) và cải thiện trong BCVA (52,8%). Kết quả được phân tầng theo giai đoạn bệnh lý và theo các biến số điều chỉnh hiệu ứng tiềm ẩn đã xác định. Chỉ có một tác dụng phụ được báo cáo ở một bệnh nhân (2,6%). KẾT LUẬN Thuốc nhỏ mắt PRGF có thể là một lựa chọn điều trị an toàn và hiệu quả cho những bệnh nhân bị NK giai đoạn 2-3, cho thấy tỷ lệ phục hồi khiếm khuyết/loét giác mạc cao trong thời gian ngắn, bằng cách giảm các dấu hiệu và triệu chứng của NK, do đó ngăn ngừa sự tiến triển của NK thành các biến chứng về mắt lớn hơn."} +{"idx": 2000103, "en": "PURPOSE The aim was to assess the repeatability of Fourier transfom analysis of videokeratographic data using Pentacam in normal (CG), keratoconic (KC) and post-CXL (CXL) corneas. METHODS This was a prospective, clinic-based, observational study. One randomly selected eye from all study participants was included in the analysis: 62 normal eyes (CG group), 33 keratoconus eyes (KC group), while 34 eyes, which had already received CXL treatment, formed the CXL group. Fourier analysis of keratometric data were obtained using Pentacam, by two different operators within each of two sessions. Precision, repeatability and Intraclass Correlation Coefficient (ICC), were calculated for evaluating intrassesion and intersession repeatability for the following parameters: Spherical Component (SphRmin, SphEcc), Maximum Decentration (Max Dec), Regular Astigmatism, and Irregularitiy (Irr). Bland-Altman analysis was used for assessing interobserver repeatability. RESULTS All parameters were presented to be repeatable, reliable and reproductible in all groups. Best intrasession and intersession repeatability and reliability were detected for parameters SphRmin, SphEcc and Max Dec parameters for both operators using ICC (intrasession: ICC > 98%, intersession: ICC > 94.7%) and within subject standard deviation. Best precision and lowest range of agreement was found for the SphRmin parameter (CG: 0.05, KC: 0.16, and CXL: 0.2) in all groups, while the lowest repeatability, reliability and reproducibility was detected for the Irr parameter. CONCLUSIONS The Pentacam system provides accurate measurements of Fourier tranform keratometric data. A single Pentacam scan will be sufficient for most clinical applications.", "gemini": "MỤC ĐÍCH Mục đích là đánh giá khả năng lặp lại của phân tích Fourier transfom về dữ liệu videokeratographic bằng Pentacam ở giác mạc bình thường (CG), hình nón (KC) và sau CXL (CXL). PHƯƠNG PHÁP Đây là một nghiên cứu quan sát, dựa trên lâm sàng, có triển vọng. Một mắt được chọn ngẫu nhiên từ tất cả những người tham gia nghiên cứu đã được đưa vào phân tích: 62 mắt bình thường (nhóm CG), 33 mắt hình nón (nhóm KC), trong khi 34 mắt đã được điều trị CXL tạo thành nhóm CXL. Phân tích Fourier của dữ liệu keratometric thu được bằng cách sử dụng Pentacam, bởi hai nhà vận hành khác nhau trong mỗi hai phiên. Độ chính xác, khả năng lặp lại và Hệ số tương quan nội lớp (ICC) đã được tính toán để đánh giá khả năng lặp lại trong phiên và giữa các phiên đối với các thông số sau: Thành phần hình cầu (SphRmin, SphEcc), Độ lệch tâm tối đa (Max Dec), Loạn thị đều và Độ bất thường (Irr). Phân tích Bland-Altman đã được sử dụng để đánh giá khả năng lặp lại giữa các quan sát viên. KẾT QUẢ Tất cả các thông số đều được trình bày là có thể lặp lại, đáng tin cậy và tái tạo được ở tất cả các nhóm. Khả năng lặp lại và độ tin cậy trong phiên và giữa các phiên tốt nhất đã được phát hiện đối với các thông số SphRmin, SphEcc và Max Dec đối với cả hai nhà vận hành sử dụng ICC (trong phiên: ICC> 98%, giữa các phiên: ICC> 94,7%) và trong độ lệch chuẩn của đối tượng. Độ chính xác tốt nhất và phạm vi đồng thuận thấp nhất được tìm thấy đối với thông số SphRmin (CG: 0,05, KC: 0,16 và CXL: 0,2) ở tất cả các nhóm, trong khi khả năng lặp lại, độ tin cậy và khả năng tái tạo thấp nhất được phát hiện đối với thông số Irr. KẾT LUẬN Hệ thống Pentacam cung cấp các phép đo chính xác về dữ liệu keratometric của Fourier tranform. Một lần quét Pentacam sẽ đủ cho hầu hết các ứng dụng lâm sàng."} +{"idx": 2000104, "en": "This review was created based on a translation of the Japanese review written in the Japanese Journal of Artificial Organs in 2015 (Vol.44, No. 3, pp.130-135), with some modifications regarding several references published in 2015 or later.", "gemini": "Bản đánh giá này được tạo ra dựa trên bản dịch từ bản đánh giá tiếng Nhật được viết trên Tạp chí Nội tạng nhân tạo của Nhật Bản năm 2015 (Tập 44, Số 3, trang 130-135), với một số sửa đổi liên quan đến một số tài liệu tham khảo được xuất bản vào năm 2015 hoặc sau đó."} +{"idx": 2000105, "en": "In this study, the variations in concentrations of some disinfection chemicals such as cyanuric acid (CyA), free chlorine (FC), and residual chlorine (RC), which affect human health, were investigated in the water of swimming pools. In addition, quality parameters such as temperature, pH, and total alkalinity (TA) in 44 swimming pools located in the city center and districts of Canakkale, Turkey, were examined in the summer period. While FC and RC amounts were analyzed using tablet tests with N-N-diethyl phenylenediamine (DPD 1) and potassium iodine (DPD 3) using the colorimetric method in the comparator, TA and CyA levels were measured with a photometric method. Temperature and pH were measured using YSI 556 MPS. Levels of CyA, FC, RC, pH, temperature, and TA varied between 0.00 and 725.0 mg L-1 (108.0 ± 111.4 mg L-1), 0.00 and 5.00 mg L-1 (1.60 ± 0.962 mg L-1), 0 and 0.55 mg L-1 (0.087 ± 0.059), 6.40 and 8.20 mg L-1 (7.30 ± 0.038 mg L-1), 22.0 and 32.0 °C (27.6 ± 1.45 °C), and 0.00 and 391.0 mg L-1 (129.3 ± 89.0), respectively. The findings were compared to standard limit values of the Ministry of Health of the Turkish Republic and other countries. Not only maximum concentrations of CyA but also the average concentrations exceeded the standard limit values of different countries in July and August with high tourism activity in Turkey. Although there is no problem in view of average values of other quality parameters, there are some problems in view of the maximum values in pool waters compared to standard limit values.", "gemini": "Trong nghiên cứu này, những biến động về nồng độ của một số hóa chất khử trùng như axit xyanuric (CyA), clo tự do (FC) và clo dư (RC), ảnh hưởng đến sức khỏe con người, đã được điều tra trong nước hồ bơi. Ngoài ra, các thông số chất lượng như nhiệt độ, pH và tổng độ kiềm (TA) trong 44 hồ bơi nằm ở trung tâm thành phố và các quận của Canakkale, Thổ Nhĩ Kỳ, đã được kiểm tra vào mùa hè. Trong khi lượng FC và RC được phân tích bằng các xét nghiệm viên nén với N-N-diethyl phenylenediamine (DPD 1) và kali iốt (DPD 3) sử dụng phương pháp so màu trong máy so màu, thì nồng độ TA và CyA được đo bằng phương pháp quang trắc. Nhiệt độ và pH được đo bằng YSI 556 MPS. Nồng độ CyA, FC, RC, pH, nhiệt độ và TA dao động trong khoảng từ 0,00 đến 725,0 mg L-1 (108,0 ± 111,4 mg L-1), 0,00 đến 5,00 mg L-1 (1,60 ± 0,962 mg L-1), 0 và 0,55 mg L-1 (0,087 ± 0,059), 6,40 và 8,20 mg L-1 (7,30 ± 0,038 mg L-1), 22,0 và 32,0 °C (27,6 ± 1,45 °C) và 0,00 và 391,0 mg L-1 (129,3 ± 89,0), tương ứng. Những phát hiện này được so sánh với các giá trị giới hạn tiêu chuẩn của Bộ Y tế Cộng hòa Thổ Nhĩ Kỳ và các quốc gia khác. Không chỉ nồng độ tối đa của CyA mà cả nồng độ trung bình đều vượt quá các giá trị giới hạn tiêu chuẩn của các quốc gia khác vào tháng 7 và tháng 8 với hoạt động du lịch cao ở Thổ Nhĩ Kỳ. Mặc dù không có vấn đề gì về giá trị trung bình của các thông số chất lượng khác, nhưng có một số vấn đề về giá trị tối đa trong nước hồ bơi so với các giá trị giới hạn tiêu chuẩn."} +{"idx": 2000106, "en": "Assessments of watershed-based restoration efforts are rare but are essential for the science of stream restoration to advance. We conducted a watershed scale assessment of Abram Creek before and after implementation of a watershed-based plan designed to maximize ecological recovery from acid mine drainage (AMD) impairment. We surveyed water chemistry, physical habitat, benthic macroinvertebrates, and fish community structure in three stream types: AMD-impacted (14 streams), AMD-treated (13 streams), and unimpaired reference (4 streams). We used in-stream measurements to quantify ecological loss from AMD, the amount of ecological recovery expected through remediation, and the observed degree of post-treatment recovery. Sites impaired by AMD improved in water quality with AMD treatment. Dissolved metals and acidity declined significantly in treated streams, but sulfate and specific conductance did not. Likewise, sites impaired by AMD improved in bio-condition scores with AMD treatment. EPT genera increased significantly but were lower compared to unimpaired streams. We found fish at nine treated sites that had none before treatment. Community-level analyses indicated improved but altered assemblages with AMD treatment. Analysis of pre-treatment conditions indicated that only 30% of the historic fishery remained. Remediation was expected to recover 66% of the historic fishery value, and assessment of post-treatment conditions indicates that 52% of the historic fishery has been recovered after 3 years. Developing expected endpoints for restoration outcomes provides a tool to objectively evaluate successes and can guide adaptive management strategies.", "gemini": "Đánh giá các nỗ lực phục hồi dựa trên lưu vực là rất hiếm nhưng rất cần thiết để khoa học phục hồi dòng chảy tiến triển. Chúng tôi đã tiến hành đánh giá quy mô lưu vực của Abram Creek trước và sau khi thực hiện một kế hoạch dựa trên lưu vực được thiết kế để tối đa hóa sự phục hồi sinh thái khỏi tình trạng suy giảm do thoát nước axit mỏ (AMD). Chúng tôi đã khảo sát hóa học nước, môi trường sống vật lý, động vật không xương sống đáy và cấu trúc quần xã cá ở ba loại dòng suối: bị AMD tác động (14 dòng suối), được xử lý AMD (13 dòng suối) và tham chiếu không bị suy giảm (4 dòng suối). Chúng tôi đã sử dụng các phép đo trong dòng suối để định lượng sự mất mát sinh thái do AMD, lượng phục hồi sinh thái dự kiến thông qua cải tạo và mức độ phục hồi sau xử lý quan sát được. Các địa điểm bị suy giảm bởi AMD được cải thiện về chất lượng nước nhờ xử lý AMD. Kim loại hòa tan và độ axit giảm đáng kể ở các dòng suối được xử lý, nhưng sunfat và độ dẫn điện riêng không giảm. Tương tự như vậy, các địa điểm bị suy giảm bởi AMD được cải thiện về điểm trạng thái sinh học nhờ xử lý AMD. Các chi EPT tăng đáng kể nhưng thấp hơn so với các dòng suối không bị suy giảm. Chúng tôi đã tìm thấy cá ở chín địa điểm được xử lý mà trước đó không có. Các phân tích ở cấp độ cộng đồng cho thấy các tập hợp được cải thiện nhưng đã thay đổi nhờ xử lý AMD. Phân tích các điều kiện trước khi xử lý chỉ ra rằng chỉ còn 30% nghề cá lịch sử. Cải tạo dự kiến sẽ phục hồi 66% giá trị nghề cá lịch sử và đánh giá các điều kiện sau xử lý cho thấy 52% nghề cá lịch sử đã được phục hồi sau 3 năm. Việc phát triển các điểm cuối mong đợi cho kết quả phục hồi cung cấp một công cụ để đánh giá khách quan những thành công và có thể hướng dẫn các chiến lược quản lý thích ứng."} +{"idx": 2000107, "en": "PURPOSE This article examines the outcome of radioactive iodine ablation therapy for thyroid cancer in high-risk patients and investigates background factors influencing ablation failure. MATERIALS AND METHODS We included 91 patients in this retrospective analysis and evaluated the ablation success rate. Successful ablation was defined as the absence of visible iodine-131 (I-131) accumulation in the thyroid bed after whole-body scans and thyroglobulin levels <2 ng/ml in a TSH-stimulated state after ablation. We extracted data on patients' age, sex, I-131 dose, pathology, resection stump findings, tumor T category and thyroglobulin levels, which could affect ablation outcome. RESULTS Successful ablation was achieved in only 14 patients (15.4%). Pre-ablation serum thyroglobulin levels were significantly higher in the ablation failure group than in the success group (P < 0.001), while no significant differences were found for other factors between the groups. Furthermore, thyroglobulin levels >10 ng/ml were significantly related to ablation failure after multivariate analysis (odds ratio 27.2; 95% confidence interval 2.469-299.7; P = 0.007). CONCLUSION The ablation success rate was very low because of high thyroglobulin levels, even with high-dose I-131. High-risk patients, especially those with high thyroglobulin levels (>10 ng/ml), are unlikely to reach levels low enough to meet successful ablation criteria.", "gemini": "MỤC ĐÍCH Bài viết này xem xét kết quả của liệu pháp cắt bỏ tuyến giáp bằng iốt phóng xạ đối với bệnh ung thư tuyến giáp ở những bệnh nhân có nguy cơ cao và điều tra các yếu tố nền ảnh hưởng đến sự thất bại của việc cắt bỏ. VẬT LIỆU VÀ PHƯƠNG PHÁP Chúng tôi đã đưa 91 bệnh nhân vào phân tích hồi cứu này và đánh giá tỷ lệ cắt bỏ thành công. Cắt bỏ thành công được định nghĩa là không có sự tích tụ iốt-131 (I-131) có thể nhìn thấy được ở vùng tuyến giáp sau khi quét toàn thân và nồng độ thyroglobulin <2 ng/ml trong trạng thái kích thích TSH sau khi cắt bỏ. Chúng tôi đã trích xuất dữ liệu về tuổi, giới tính, liều I-131, bệnh lý, phát hiện gốc cắt bỏ, loại T khối u và nồng độ thyroglobulin của bệnh nhân, những yếu tố có thể ảnh hưởng đến kết quả cắt bỏ. KẾT QUẢ Chỉ có 14 bệnh nhân (15,4%) cắt bỏ thành công. Nồng độ thyroglobulin huyết thanh trước khi cắt bỏ cao hơn đáng kể ở nhóm cắt bỏ thất bại so với nhóm thành công (P < 0,001), trong khi không tìm thấy sự khác biệt đáng kể nào về các yếu tố khác giữa các nhóm. Hơn nữa, nồng độ thyroglobulin> 10 ng/ml có liên quan đáng kể đến sự thất bại của việc cắt bỏ sau phân tích đa biến (tỷ lệ cược 27,2; khoảng tin cậy 95% 2,469-299,7; P = 0,007). KẾT LUẬN Tỷ lệ cắt bỏ thành công rất thấp do nồng độ thyroglobulin cao, ngay cả khi sử dụng I-131 liều cao. Những bệnh nhân có nguy cơ cao, đặc biệt là những bệnh nhân có nồng độ thyroglobulin cao (> 10 ng/ml), không có khả năng đạt được mức đủ thấp để đáp ứng các tiêu chí cắt bỏ thành công."} +{"idx": 2000108, "en": "This Q methodological study was conducted to examine the perspectives of health professionals in providing sexual and reproductive health (SRH) care to refugee and migrant women. Forty-seven health professionals rank-ordered 42 statements and commented on their rankings in subsequent open-ended questions. A bi-person factor analysis was performed and factors were extracted according to the centroid method with a varimax rotation. Seven factors each with a distinct and meaningful viewpoint were identified. These factors are: \"Communication difficulties-hurdles to counselling\", \"Lack of access to culturally appropriate care\", \"Navigating SRH care\", \"Cultural constraints on effective communication\", \"Effects of the lack of cultural competency\", \"Impacts of low income and language barrier\" and \"SRH services are accessible, but not culturally relevant\". A more culturally adaptive healthcare model that considers refugee and migrant women's linguistic, cultural and socio-economic backgrounds; and engages health professionals on an ongoing process of building cultural competency is central to improve SRH access to these women.", "gemini": "Nghiên cứu phương pháp Q này được tiến hành để kiểm tra quan điểm của các chuyên gia y tế trong việc cung cấp dịch vụ chăm sóc sức khỏe tình dục và sinh sản (SRH) cho phụ nữ tị nạn và di cư. Bốn mươi bảy chuyên gia y tế đã xếp hạng 42 tuyên bố và bình luận về thứ hạng của họ trong các câu hỏi mở tiếp theo. Một phân tích nhân tố hai người đã được thực hiện và các nhân tố được trích xuất theo phương pháp trọng tâm với phép quay varimax. Bảy nhân tố, mỗi nhân tố có một quan điểm riêng biệt và có ý nghĩa đã được xác định. Các yếu tố này là: \"Khó khăn trong giao tiếp - rào cản đối với tư vấn\", \"Thiếu khả năng tiếp cận dịch vụ chăm sóc phù hợp về mặt văn hóa\", \"Dẫn đường chăm sóc SRH\", \"Ràng buộc văn hóa đối với giao tiếp hiệu quả\", \"Ảnh hưởng của việc thiếu năng lực văn hóa\", \"Tác động của thu nhập thấp và rào cản ngôn ngữ\" và \"Các dịch vụ SRH có thể tiếp cận được, nhưng không phù hợp về mặt văn hóa\". Một mô hình chăm sóc sức khỏe thích ứng hơn về mặt văn hóa, xem xét bối cảnh ngôn ngữ, văn hóa và kinh tế xã hội của phụ nữ tị nạn và di cư; và việc thu hút các chuyên gia y tế vào quá trình liên tục xây dựng năng lực văn hóa là trọng tâm để cải thiện khả năng tiếp cận SRH đối với những phụ nữ này."} +{"idx": 2000109, "en": "BACKGROUND Hepatitis B virus (HBV) and hepatitis C virus (HCV) infections are widespread health problems all over the world and have high morbidity and mortality. Hemodialysis patients are more frequently exposed to these viruses as they have poor immune system and frequently undergo parenteral interventions. The vaccination against HBV prevents infection and it has been recommended for the prevention of HBV infection in all susceptible dialysis patients. This study aimed to determine the seroprevalence of HBV and HCV infections and analyzed the factors affecting inadequate response to HBV vaccine in dialysis patients. METHODS The data for 584 dialysis patients that were followed up at seven dialysis centers were analyzed. The patients received four doses of 40 μg recombinant hepatitis B vaccine at 0, 1, 2, and 6 months and were tested for anti-HBs titer after one month of completion of vaccination. If patients showed a titer of anti-HBs <10 IU/mL, an additional 40 μg in four vaccine doses was administered immediately and they were retested for the anti-HBs following the same schedule. The patients were divided into two groups: responders and non-responders. RESULTS HBsAg and anti-HCV seroprevalence was 3.4% and 10.3%, respectively. After vaccination schedule, 264 (83.5%) patients had antibody response to HBV vaccine and 52 (16.5%) had no response. Hepatitis B vaccine unresponsiveness is more common in the patients with hepatitis C positivity (p = 0.011), BMI >30 (p = 0.019), over the age of 65 years (p = 0.009), and duration of dialysis treatment >5 years (p = 0.001). There was no statistically significant difference between gender, causes of renal disease, erythropoietin treatment, and the type of dialysis. CONCLUSION Hepatitis C infection, obesity, being elderly, and having long hemodialysis period reduced the hepatitis B vaccination response in hemodialysis patients.", "gemini": "BỐI CẢNH Nhiễm vi-rút viêm gan B (HBV) và vi-rút viêm gan C (HCV) là những vấn đề sức khỏe phổ biến trên toàn thế giới và có tỷ lệ mắc bệnh và tử vong cao. Bệnh nhân chạy thận nhân tạo thường xuyên tiếp xúc với những loại vi-rút này vì họ có hệ thống miễn dịch kém và thường xuyên phải can thiệp qua đường tiêm. Việc tiêm vắc-xin phòng ngừa HBV giúp ngăn ngừa nhiễm trùng và được khuyến cáo để phòng ngừa nhiễm HBV ở tất cả những bệnh nhân chạy thận nhân tạo dễ mắc bệnh. Nghiên cứu này nhằm xác định tỷ lệ huyết thanh dương tính của nhiễm HBV và HCV và phân tích các yếu tố ảnh hưởng đến đáp ứng không đầy đủ với vắc-xin HBV ở bệnh nhân chạy thận nhân tạo. PHƯƠNG PHÁP Dữ liệu của 584 bệnh nhân chạy thận nhân tạo được theo dõi tại bảy trung tâm chạy thận nhân tạo đã được phân tích. Các bệnh nhân đã được tiêm bốn liều vắc-xin viêm gan B tái tổ hợp 40 μg tại thời điểm 0, 1, 2 và 6 tháng và được xét nghiệm định lượng anti-HBs sau một tháng hoàn thành tiêm chủng. Nếu bệnh nhân có giá trị định lượng anti-HBs <10 IU/mL, sẽ được tiêm thêm 4 liều vắc-xin 40 μg ngay lập tức và được xét nghiệm lại anti-HBs theo cùng một lịch trình. Các bệnh nhân được chia thành hai nhóm: nhóm đáp ứng và nhóm không đáp ứng. KẾT QUẢ Tỷ lệ huyết thanh dương tính với HBsAg và anti-HCV lần lượt là 3,4% và 10,3%. Sau lịch tiêm chủng, 264 (83,5%) bệnh nhân có đáp ứng kháng thể với vắc-xin HBV và 52 (16,5%) không có đáp ứng. Tỷ lệ không đáp ứng với vắc-xin viêm gan B phổ biến hơn ở những bệnh nhân dương tính với viêm gan C (p = 0,011), BMI> 30 (p = 0,019), trên 65 tuổi (p = 0,009) và thời gian điều trị chạy thận nhân tạo > 5 năm (p = 0,001). Không có sự khác biệt có ý nghĩa thống kê giữa giới tính, nguyên nhân gây bệnh thận, điều trị erythropoietin và loại chạy thận nhân tạo. KẾT LUẬN Nhiễm vi-rút viêm gan C, béo phì, lớn tuổi và thời gian chạy thận nhân tạo kéo dài làm giảm đáp ứng tiêm vắc-xin viêm gan B ở bệnh nhân chạy thận nhân tạo."} +{"idx": 2000110, "en": "The C9orf72 repeat expansion (RE) is one of the most frequent causative mutations of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). However, it is still unclear how the C9orf72 RE can lead to a heterogeneous phenotype. Several reports have shown the coexistence of mutations in multiple ALS/FTD causative genes in the same family, suggesting an oligogenic etiology for ALS and FTD. Our aim was to investigate this phenomenon in an Italian group of ALS/FTD pedigrees carrying the C9orf72 RE. We included 11 subjects from 11 pedigrees with ALS/FTD and the C9orf72 RE. Mutation screening of FUS, SOD1 and TARDBP genes was performed by direct sequencing. A dementia-specific custom-designed targeted next-generation sequencing panel was used for screening dementia-associated genes mutations. We found genetic variants in additional ALS or dementia-related genes in four pedigrees, including the p.V47A variant in the TYROBP gene. As a group, double mutation carriers displayed a tendency toward a younger age at onset and a higher frequency of positive familiar history and of parkinsonism. Our observation supports the hypothesis that the co-presence of mutations in different genes may be relevant for the clinical expression of ALS/FTD and of their oligogenic nature.", "gemini": "Sự mở rộng lặp lại C9orf72 (RE) là một trong những đột biến gây bệnh thường gặp nhất của bệnh xơ cứng teo cơ một bên (ALS) và chứng mất trí trán thái dương (FTD). Tuy nhiên, vẫn chưa rõ làm thế nào C9orf72 RE có thể dẫn đến kiểu hình không đồng nhất. Một số báo cáo đã chỉ ra sự đồng thời tồn tại của các đột biến ở nhiều gen gây ALS/FTD trong cùng một gia đình, gợi ý về nguyên nhân di truyền học cho ALS và FTD. Mục tiêu của chúng tôi là nghiên cứu hiện tượng này trong một nhóm phả hệ ALS/FTD của Ý mang C9orf72 RE. Chúng tôi đã đưa vào 11 đối tượng từ 11 phả hệ mắc ALS/FTD và C9orf72 RE. Kiểm tra đột biến của các gen FUS, SOD1 và TARDBP được thực hiện bằng phương pháp giải trình tự trực tiếp. Một bảng giải trình tự mục tiêu thế hệ tiếp theo được thiết kế riêng cho chứng mất trí đã được sử dụng để sàng lọc các đột biến gen liên quan đến chứng mất trí. Chúng tôi đã tìm thấy các biến thể di truyền ở các gen liên quan đến ALS hoặc chứng mất trí khác trong bốn phả hệ, bao gồm biến thể p.V47A trong gen TYROBP. Nhìn chung, những người mang đột biến kép có xu hướng khởi phát bệnh ở độ tuổi trẻ hơn và có tần suất tiền sử gia đình mắc bệnh và bệnh Parkinson cao hơn. Quan sát của chúng tôi ủng hộ giả thuyết rằng sự đồng hiện của các đột biến ở các gen khác nhau có thể có liên quan đến biểu hiện lâm sàng của ALS/FTD và bản chất di truyền học của chúng."} +{"idx": 2000111, "en": "Mutations or structural genomic alterations of the X-chromosomal gene ARHGEF9 have been described in male and female patients with intellectual disability. Hyperekplexia and epilepsy were observed to a variable degree, but incompletely described. Here, we expand the phenotypic spectrum of ARHGEF9 by describing a large Ethiopian-Jewish family with epilepsy and intellectual disability. The four affected male siblings, their unaffected parents and two unaffected female siblings were recruited and phenotyped. Parametric linkage analysis was performed using SNP microarrays. Variants from exome sequencing in two affected individuals were confirmed by Sanger sequencing. All affected male siblings had febrile seizures from age 2-3 years and intellectual disability. Three developed afebrile seizures between age 7-17 years. Three showed focal seizure semiology. None had hyperekplexia. A novel ARHGEF9 variant (c.967G>A, p.G323R, NM_015185.2) was hemizygous in all affected male siblings and heterozygous in the mother. This family reveals that the phenotypic spectrum of ARHGEF9 is broader than commonly assumed and includes febrile seizures and focal epilepsy with intellectual disability in the absence of hyperekplexia or other clinically distinguishing features. Our findings suggest that pathogenic variants in ARHGEF9 may be more common than previously assumed in patients with intellectual disability and mild epilepsy.", "gemini": "Các đột biến hoặc các biến đổi cấu trúc bộ gen của gen ARHGEF9 nhiễm sắc thể giới tính X đã được mô tả ở bệnh nhân nam và nữ bị khuyết tật trí tuệ. Tình trạng tăng phản xạ giật mình và động kinh được quan sát ở các mức độ khác nhau, nhưng không được mô tả đầy đủ. Ở đây, chúng tôi mở rộng phổ biểu hiện kiểu hình của ARHGEF9 bằng cách mô tả một gia đình Do Thái-Ethiopia lớn bị động kinh và khuyết tật trí tuệ. Bốn anh em trai bị ảnh hưởng, cha mẹ không bị ảnh hưởng và hai chị gái không bị ảnh hưởng đã được tuyển dụng và lập biểu hiện kiểu hình. Phân tích liên kết tham số được thực hiện bằng cách sử dụng mảng vi SNP. Các biến thể từ giải trình tự toàn bộ exome ở hai cá thể bị ảnh hưởng đã được xác nhận bằng giải trình tự Sanger. Tất cả các anh em trai bị ảnh hưởng đều bị co giật do sốt từ 2-3 tuổi và khuyết tật trí tuệ. Ba người phát triển các cơn động kinh không sốt trong độ tuổi từ 7-17. Ba người biểu hiện triệu chứng động kinh cục bộ. Không ai bị tăng phản xạ giật mình. Một biến thể ARHGEF9 mới (c.967G>A, p.G323R, NM_015185.2) là hemizygot ở tất cả các anh em trai bị ảnh hưởng và dị hợp tử ở người mẹ. Gia đình này cho thấy rằng phổ biểu hiện kiểu hình của ARHGEF9 rộng hơn so với thông thường và bao gồm các cơn động kinh do sốt và động kinh cục bộ với khuyết tật trí tuệ khi không có tăng phản xạ giật mình hoặc các đặc điểm phân biệt lâm sàng khác. Những phát hiện của chúng tôi cho thấy rằng các biến thể gây bệnh ở ARHGEF9 có thể phổ biến hơn so với trước đây ở những bệnh nhân bị khuyết tật trí tuệ và động kinh nhẹ."} +{"idx": 2000112, "en": "The objective of this study was to evaluate large coverage magnetic resonance neurography (MRN) in chronic inflammatory demyelinating polyneuropathy (CIDP). In this prospective study, 18 patients with CIDP and 18 healthy controls were examined by a standardized MRN protocol at 3 T. Lumbosacral plexus was imaged by a T2-weighted 3D sequence and peripheral nerves of the upper and lower extremity by axial T2-weighted turbo spin-echo sequences. Lesions were characterized by nerve cross-sectional area (CSA) and T2-weighted signal (nT2). Additionally, T2 relaxometry of the sciatic nerve was performed using a multi-spin-echo sequence. All patients received a complementary electrophysiological exam. Patients with CIDP exhibited increased nerve CSA and nT2 compared to controls (p < 0.05) in a proximally predominating pattern. Receiver operating characteristic analysis revealed the best diagnostic accuracy for CSA of the lumbosacral plexus (AUC = 0.88) and nT2 of the sciatic nerve (AUC = 0.88). CSA correlated with multiple electrophysiological parameters of demyelinating neuropathy (F wave latency, nerve conduction velocity) of sciatic and median nerve, while nT2 only correlated with F wave latency of sciatic and not median nerve. T2 relaxometry indicated that MR signal increase in CIDP was due to an increase in proton-spin-density (p < 0.05), and not due to the increase in T2 relaxation time. Both nT2 and CSA might aid in the diagnosis of CIDP, but CSA correlates more robustly with established electrophysiological parameters for CIDP. Since the best diagnostic accuracy was shown for proximal nerve locations, MRN may be a useful complementary tool in selected CIDP cases.", "gemini": "Mục tiêu của nghiên cứu này là đánh giá kỹ thuật chụp thần kinh cộng hưởng từ (MRN) phủ sóng rộng trong bệnh đa dây thần kinh viêm mất myelin mạn tính (CIDP). Trong nghiên cứu triển vọng này, 18 bệnh nhân bị CIDP và 18 người đối chứng khỏe mạnh đã được kiểm tra bằng giao thức MRN chuẩn hóa ở 3 T. Búi thần kinh thắt lưng cùng được chụp bằng chuỗi 3D có trọng số T2 và các dây thần kinh ngoại biên của chi trên và chi dưới bằng các chuỗi hồi chuyển hồi âm nhanh có trọng số T2 theo trục. Các tổn thương được đặc trưng bởi diện tích mặt cắt ngang (CSA) của dây thần kinh và tín hiệu có trọng số T2 (nT2). Ngoài ra, kỹ thuật đo độ giãn T2 của dây thần kinh tọa đã được thực hiện bằng cách sử dụng chuỗi đa hồi chuyển hồi âm. Tất cả bệnh nhân đều được kiểm tra điện sinh lý bổ sung. Bệnh nhân bị CIDP biểu hiện CSA và nT2 của dây thần kinh tăng lên so với nhóm đối chứng (p < 0,05) theo kiểu chủ yếu ở gần. Phân tích đặc điểm hoạt động của máy thu cho thấy độ chính xác chẩn đoán tốt nhất đối với CSA của đám rối thắt lưng cùng (AUC = 0,88) và nT2 của dây thần kinh tọa (AUC = 0,88). CSA tương quan với nhiều thông số điện sinh lý của bệnh lý thần kinh mất myelin (độ trễ sóng F, vận tốc dẫn truyền thần kinh) của dây thần kinh tọa và dây thần kinh giữa, trong khi nT2 chỉ tương quan với độ trễ sóng F của dây thần kinh tọa chứ không phải dây thần kinh giữa. Đo độ giãn T2 chỉ ra rằng sự gia tăng tín hiệu MR trong CIDP là do sự gia tăng mật độ spin proton (p < 0,05), chứ không phải do sự gia tăng thời gian giãn T2. Cả nT2 và CSA đều có thể hỗ trợ chẩn đoán CIDP, nhưng CSA tương quan chặt chẽ hơn với các thông số điện sinh lý đã được thiết lập cho CIDP. Vì độ chính xác chẩn đoán tốt nhất được thể hiện ở các vị trí dây thần kinh gần, nên MRN có thể là một công cụ bổ sung hữu ích trong các trường hợp CIDP được chọn."} +{"idx": 2000113, "en": "We report three affected members, a mother and her two children, of a non-consanguineous Irish family who presented with a suspected autosomal dominant spinocerebellar ataxia characterized by early motor delay, poor coordination, gait ataxia, and dysarthria. Whole exome sequencing identified a novel missense variant (c.106C>T; p.[Arg36Cys]) in the suppressor domain of type 1 inositol 1,4,5-trisphosphate receptor gene (ITPR1) as the cause of the disorder, resulting in a molecular diagnosis of spinocerebellar ataxia type 29. In the absence of grandparental DNA, microsatellite genotyping of healthy family members was used to confirm the de novo status of the ITPR1 variant in the affected mother, which supported pathogenicity. The Arg36Cys variant exhibited a significantly higher IP3-binding affinity than wild-type (WT) ITPR1 and drastically changed the property of the intracellular Ca2+ signal from a transient to a sigmoidal pattern, supporting a gain-of-function disease mechanism. To date, ITPR1 mutation has been associated with a loss-of-function effect, likely due to reduced Ca2+ release. This is the first gain-of-function mechanism to be associated with ITPR1-related SCA29, providing novel insights into how enhanced Ca2+ release can also contribute to the pathogenesis of this neurological disorder.", "gemini": "Chúng tôi báo cáo ba thành viên bị ảnh hưởng, một người mẹ và hai đứa con của một gia đình người Ireland không cận huyết thống, những người xuất hiện với nghi ngờ chứng mất điều hòa tiểu não trội trên nhiễm sắc thể thường đặc trưng bởi chậm phát triển vận động sớm, phối hợp kém, dáng đi không vững và nói khó. Giải trình tự toàn bộ exome xác định một biến thể sai nghĩa mới (c.106C>T; p.[Arg36Cys]) trong miền ức chế của thụ thể inositol 1,4,5-trisphosphate loại 1 (ITPR1) là nguyên nhân gây ra rối loạn, dẫn đến chẩn đoán phân tử là chứng mất điều hòa tiểu não loại 29. Trong trường hợp không có DNA của ông bà, kiểu gen vi vệ tinh của các thành viên gia đình khỏe mạnh đã được sử dụng để xác nhận trạng thái de novo của biến thể ITPR1 ở người mẹ bị ảnh hưởng, điều này hỗ trợ cho tính gây bệnh. Biến thể Arg36Cys biểu hiện ái lực liên kết IP3 cao hơn đáng kể so với ITPR1 kiểu hoang dã (WT) và thay đổi mạnh mẽ tính chất của tín hiệu Ca2+ trong tế bào từ kiểu thoáng qua sang kiểu hình sin, hỗ trợ cơ chế gây bệnh tăng chức năng. Cho đến nay, đột biến ITPR1 đã được liên kết với tác dụng mất chức năng, có thể là do giải phóng Ca2+ giảm. Đây là cơ chế tăng chức năng đầu tiên được liên kết với SCA29 liên quan đến ITPR1, cung cấp những hiểu biết mới về cách giải phóng Ca2+ tăng cường cũng có thể góp phần vào quá trình sinh bệnh của rối loạn thần kinh này."} +{"idx": 2000114, "en": "The marijuana amotivational syndrome posits that cannabis use fosters apathy through the depletion of motivation-based constructs such as self-efficacy. The current study pursued a two-round design to rule out concomitant risk factors responsible for the connection from marijuana intake to lower general self-efficacy. College students (N = 505) completed measures of marijuana use, demographics (age, gender, and race), personality (extraversion, agreeableness, conscientiousness, openness, and neuroticism), other substance use (alcohol and tobacco), and general self-efficacy (initiative, effort, and persistence) in two assessments separated by a month. Hierarchical regression models found that marijuana use forecasted lower initiative and persistence, even after statistically ruling out 13 pertinent baseline covariates including demographics, personality traits, alcohol use, tobacco use, and self-efficacy subscales. A cross-lagged panel model involving initiative, effort, persistence, alcohol use, cigarette use, and marijuana use sought to unravel the temporal precedence of processes. Results showed that only marijuana (but not alcohol or tobacco) intake significantly and longitudinally prompted lower initiative and persistence. Furthermore, in the same model, the opposite temporal direction of events from lower general self-efficacy subscales to marijuana use was untenable. Findings provide partial support for the marijuana amotivational syndrome, underscore marijuana as a risk factor for decreased general self-efficacy, and offer implications and insights for marijuana prevention and future research.", "gemini": "Hội chứng mất động lực do cần sa đưa ra giả thuyết rằng việc sử dụng cần sa thúc đẩy sự thờ ơ thông qua sự cạn kiệt các cấu trúc dựa trên động lực như hiệu quả bản thân. Nghiên cứu hiện tại theo đuổi một thiết kế hai vòng để loại trừ các yếu tố rủi ro đồng thời chịu trách nhiệm cho mối liên hệ từ việc sử dụng cần sa đến hiệu quả bản thân chung thấp hơn. Sinh viên đại học (N = 505) đã hoàn thành các biện pháp sử dụng cần sa, thông tin nhân khẩu (tuổi, giới tính và chủng tộc), tính cách (hướng ngoại, dễ chịu, tận tâm, cởi mở và hay lo lắng), sử dụng chất khác (rượu và thuốc lá) và hiệu quả bản thân chung (sáng kiến, nỗ lực và kiên trì) trong hai lần đánh giá cách nhau một tháng. Các mô hình hồi quy phân cấp cho thấy việc sử dụng cần sa dự báo sáng kiến và sự kiên trì thấp hơn, ngay cả sau khi loại trừ về mặt thống kê 13 biến phụ cơ sở có liên quan bao gồm thông tin nhân khẩu, đặc điểm tính cách, sử dụng rượu, sử dụng thuốc lá và các thang đo phụ về hiệu quả bản thân. Một mô hình bảng chéo liên quan đến sáng kiến, nỗ lực, kiên trì, sử dụng rượu, sử dụng thuốc lá và sử dụng cần sa nhằm mục đích làm sáng tỏ thứ tự thời gian của các quá trình. Kết quả cho thấy chỉ có lượng cần sa (nhưng không phải rượu hoặc thuốc lá) mới có ý nghĩa và theo chiều dọc thúc đẩy sáng kiến và sự kiên trì thấp hơn. Hơn nữa, trong cùng một mô hình, hướng thời gian ngược lại của các sự kiện từ các thang đo phụ về hiệu quả bản thân chung thấp hơn đến việc sử dụng cần sa là không thể chấp nhận được. Các phát hiện cung cấp sự hỗ trợ một phần cho hội chứng mất động lực do cần sa, nhấn mạnh cần sa là một yếu tố rủi ro dẫn đến giảm hiệu quả bản thân chung và đưa ra những hàm ý và hiểu biết sâu sắc cho việc phòng ngừa cần sa và nghiên cứu trong tương lai."} +{"idx": 2000115, "en": "This introduction examines major issues and challenges as presented in this special issue of Prevention Science, \"Challenges to the Dissemination and Implementation of Evidence Based Prevention Interventions for Diverse Populations.\" We describe the Fidelity-Adaptation Dilemma that became the origin of dynamic tensions in prevention science. It generated controversies and debates and new perspectives on the dissemination and implementation of evidence-based interventions (EBIs) within diverse populations. The five articles in this special issue address many of these challenges. These dynamic tensions have culminated in a reframing of this dilemma that now argues that fidelity and adaptation are both equally important imperatives. These five articles also examine the abiding challenges of engagement and sustainability. Also, two commentaries from expert research investigators reflect on these five articles and their contributions to prevention science. The current introduction describes this \"second generation\" of EBIs as they may contribute towards a reduction of the health disparities and inequities that disproportionately affect sectors of the major ethnocultural populations in the USA. We also present a systems analysis of approaches and challenges in the dissemination and implementation of EBIs within the contemporary integrative care environment. Finally, we mention an initiative for, \"building a science of intervention adaptation,\" that proposes systematic research and the creation of an archive of scientific data on the benefits and problems of intervention adaptation. Collectively, these new directions can integrate scientific rigor and sensitivity to cultural factors, for enhancing the effectiveness and reach of this second generation of evidence-based interventions with diverse ethnocultural populations.", "gemini": "Phần giới thiệu này xem xét các vấn đề và thách thức chính như được trình bày trong số đặc biệt này của Khoa học phòng ngừa, \"Những thách thức đối với việc phổ biến và triển khai các biện pháp can thiệp phòng ngừa dựa trên bằng chứng cho các nhóm dân số đa dạng\". Chúng tôi mô tả Nghịch lý về lòng trung thành và sự thích ứng đã trở thành nguồn gốc của những căng thẳng năng động trong khoa học phòng ngừa. Nó đã tạo ra những tranh cãi và tranh luận cũng như những góc nhìn mới về việc phổ biến và triển khai các biện pháp can thiệp dựa trên bằng chứng (EBI) trong các nhóm dân số đa dạng. Năm bài báo trong số đặc biệt này giải quyết nhiều trong số những thách thức này. Những căng thẳng năng động này đã lên đến đỉnh điểm trong việc định hình lại tình thế tiến thoái lưỡng nan này, hiện cho rằng cả lòng trung thành và sự thích ứng đều là những mệnh lệnh quan trọng như nhau. Năm bài báo này cũng xem xét những thách thức lâu dài về sự tham gia và tính bền vững. Ngoài ra, hai bình luận từ các nhà nghiên cứu chuyên gia phản ánh về năm bài báo này và những đóng góp của họ cho khoa học phòng ngừa. Phần giới thiệu hiện tại mô tả \"thế hệ thứ hai\" của EBI này vì chúng có thể đóng góp vào việc giảm sự chênh lệch và bất bình đẳng về sức khỏe ảnh hưởng không cân xứng đến các khu vực của các nhóm dân tộc văn hóa chính ở Hoa Kỳ. Chúng tôi cũng trình bày một phân tích hệ thống về các cách tiếp cận và thách thức trong việc phổ biến và triển khai EBI trong môi trường chăm sóc tích hợp đương đại. Cuối cùng, chúng tôi đề cập đến một sáng kiến, \"xây dựng khoa học về sự thích ứng của biện pháp can thiệp\", đề xuất nghiên cứu có hệ thống và tạo ra một kho lưu trữ dữ liệu khoa học về lợi ích và vấn đề của sự thích ứng của biện pháp can thiệp. Nhìn chung, những hướng đi mới này có thể tích hợp tính nghiêm ngặt khoa học và sự nhạy cảm với các yếu tố văn hóa, nhằm nâng cao hiệu quả và phạm vi tiếp cận của thế hệ thứ hai này của các biện pháp can thiệp dựa trên bằng chứng với các nhóm dân tộc văn hóa đa dạng."} +{"idx": 2000116, "en": "Primary care practices have increasingly adopted the patient-centered medical home (PCMH) model and often adapted quality improvement efforts to fit local context. This paper implemented a modified framework for understanding adaptations in the context of primary care PCMH transformation efforts. We combined an adaptations model by Stirman et al. that categorized adaptations to evidence-based interventions in research studies with dimensions from the RE-AIM framework, as well as items specific to PCMH. The resulting constructs were translated into a \"plain English\" adaptations interview. We conducted interviews with 27 practices and used resulting descriptive categories to inform exploratory analyses of the relationships between adaptation characteristics and improvement outcomes in PCMH domains of team-based care and data capacity. Practices most commonly focused on development and use of disease registries and enhancements to team-based care (not disease-specific outcomes). Adaptations were common, with practices most frequently making changes to format or personnel. Adaptations were most often intended to increase effectiveness and based on pragmatic considerations. Generally similar adaptation themes emerged across different content topics (registry and quality improvement team). Adaptations initiated or carried out by the entire team or made in early to middle stages of the project were most related to outcome measures of team-based care and data capacity. This paper extends adaptation models from specific interventions in research studies to PCMH quality improvement efforts. Despite limitations, the PCMH Adaptations Model provided a useful framework to understand adaptations in this context.", "gemini": "Các hoạt động chăm sóc chính ngày càng áp dụng mô hình nhà y tế lấy bệnh nhân làm trung tâm (PCMH) và thường điều chỉnh các nỗ lực cải thiện chất lượng cho phù hợp với bối cảnh địa phương. Bài báo này đã triển khai một khuôn khổ đã sửa đổi để hiểu các sự thích ứng trong bối cảnh các nỗ lực chuyển đổi PCMH chăm sóc chính. Chúng tôi đã kết hợp một mô hình thích ứng của Stirman cộng sự đã phân loại các sự thích ứng với các biện pháp can thiệp dựa trên bằng chứng trong các nghiên cứu với các chiều từ khuôn khổ RE-AIM, cũng như các mục cụ thể cho PCMH. Các cấu trúc kết quả đã được dịch thành một cuộc phỏng vấn về sự thích ứng \"tiếng Anh đơn giản\". Chúng tôi đã tiến hành phỏng vấn với 27 phòng khám và sử dụng các danh mục mô tả kết quả để thông báo cho các phân tích thăm dò về mối quan hệ giữa các đặc điểm thích ứng và kết quả cải thiện trong các lĩnh vực PCMH về chăm sóc theo nhóm và năng lực dữ liệu. Các hoạt động thường tập trung nhất vào việc phát triển và sử dụng sổ đăng ký bệnh và nâng cao khả năng chăm sóc theo nhóm (không phải kết quả cụ thể của bệnh). Sự thích ứng là phổ biến, với các hoạt động thường xuyên nhất là thay đổi định dạng hoặc nhân sự. Sự thích ứng thường có mục đích tăng hiệu quả và dựa trên những cân nhắc thực tế. Nhìn chung, các chủ đề thích ứng tương tự xuất hiện trên các chủ đề nội dung khác nhau (sổ đăng ký và nhóm cải tiến chất lượng). Các sự thích ứng do toàn bộ nhóm khởi xướng hoặc thực hiện hoặc được thực hiện trong giai đoạn đầu đến giữa của dự án có liên quan nhiều nhất đến các biện pháp kết quả về chăm sóc theo nhóm và năng lực dữ liệu. Bài báo này mở rộng các mô hình thích ứng từ các biện pháp can thiệp cụ thể trong các nghiên cứu sang các nỗ lực cải thiện chất lượng PCMH. Mặc dù có những hạn chế, Mô hình thích ứng PCMH đã cung cấp một khuôn khổ hữu ích để hiểu các sự thích ứng trong bối cảnh này."} +{"idx": 2000117, "en": "Concentration of Cd, Cr, Ni, and Pb were determined in peppermint leaves (Folium Menthae piperitae), chamomile blossom (Anthodium Chamomillae), and their infusions by atomic absorption spectrometry (AAS) after microwave digestion of plants samples. Peppermint and chamomile preparations by five different producers were purchased at various pharmacies in Poland. Infusions were made from herbs according to prescription for patients, provided by the producer of medicine on the package. Results show that in exam of medicinal plants the content of cadmium exceeds World Health Organization (WHO) standards. The highest level of extraction was observed for Ni (50-70% of the total content in herb), Cr (28-36%), Cd (14-16%), and the lowest for Pb (about 6%). The calculated daily intake all analyzed elements was very low. Phytotherapy with using of these herbs is safe for health of patient even in case of long time therapy.", "gemini": "Nồng độ Cd, Cr, Ni và Pb được xác định trong lá bạc hà (Folium Menthae piperitae), hoa cúc (Anthodium Chamomillae) và dịch truyền của chúng bằng phương pháp quang phổ hấp thụ nguyên tử (AAS) sau khi tiêu hủy bằng lò vi sóng các mẫu thực vật. Các chế phẩm bạc hà và hoa cúc của năm nhà sản xuất khác nhau đã được mua tại các hiệu thuốc khác nhau ở Ba Lan. Dịch truyền được làm từ các loại thảo mộc theo đơn thuốc cho bệnh nhân, do nhà sản xuất thuốc cung cấp trên bao bì. Kết quả cho thấy khi kiểm tra các loại dược liệu thì hàm lượng cadimi vượt quá tiêu chuẩn của Tổ chức Y tế Thế giới (WHO). Mức chiết xuất cao nhất được quan sát thấy đối với Ni (50-70% tổng hàm lượng trong thảo mộc), Cr (28-36%), Cd (14-16%) và thấp nhất đối với Pb (khoảng 6%). Lượng hấp thụ hàng ngày được tính toán của tất cả các nguyên tố được phân tích đều rất thấp. Liệu pháp thảo dược bằng cách sử dụng các loại thảo mộc này là an toàn cho sức khỏe của bệnh nhân ngay cả trong trường hợp điều trị lâu dài."} +{"idx": 2000118, "en": "Fresh Mashui orange samples were pretreated with microwave digestion using an HNO3-H2O2 system. The levels of Mg, K, Ca, Fe, Mn, Cu, Zn, As, Cd, and Pb in the seeds, pulp, and peel were then determined using inductively coupled plasma mass spectrometry (ICP-MS) combined with collision cell technology (CCT) and kinetic energy discrimination (KED). The standard curve coefficient of determinations of the ten tested elements were between 0.9995 and 0.9999. The instrument detection limit was between 0.112 ng/L and 3.05 ng/mL. The method detection limit was between 0.0281 and 763 ng/g. The average recovery rate was between 85.0 and 117%. The current results showed that Mashui oranges are rich in three elements, namely Mg, K, and Ca. The concentrations of K and Ca were significantly higher than that of Mg in the peel. The content of K was the highest in the seeds. Fe, Mn, Cu, and Zn had the second highest concentrations, and Fe was the highest in the seeds, while Cu was the lowest in the peel. As, Cd, and Pb (hazardous elements) had the lowest concentrations of all the tested elements.", "gemini": "Các mẫu cam Mashui tươi đã được xử lý sơ bộ bằng cách tiêu hủy bằng lò vi sóng sử dụng hệ thống HNO3-H2O2. Sau đó, nồng độ Mg, K, Ca, Fe, Mn, Cu, Zn, As, Cd và Pb trong hạt, cùi và vỏ được xác định bằng phương pháp phổ khối lượng plasma ghép cảm ứng (ICP-MS) kết hợp với công nghệ tế bào va chạm (CCT) và phân biệt năng lượng động học (KED). Hệ số đường chuẩn xác định của mười nguyên tố được thử nghiệm nằm trong khoảng 0,9995 và 0,9999. Giới hạn phát hiện của thiết bị nằm trong khoảng 0,112 ng/L và 3,05 ng/mL. Giới hạn phát hiện của phương pháp nằm trong khoảng 0,0281 và 763 ng/g. Tỷ lệ thu hồi trung bình nằm trong khoảng 85,0 và 117%. Kết quả hiện tại cho thấy cam Mashui rất giàu ba nguyên tố là Mg, K và Ca. Nồng độ K và Ca cao hơn đáng kể so với Mg trong vỏ. Hàm lượng K cao nhất trong hạt. Fe, Mn, Cu và Zn có nồng độ cao thứ hai, trong đó Fe cao nhất trong hạt, trong khi Cu thấp nhất trong vỏ. As, Cd và Pb (các nguyên tố nguy hiểm) có nồng độ thấp nhất trong tất cả các nguyên tố được thử nghiệm."} +{"idx": 2000119, "en": "Toxic heavy metal contamination in Chinese edible herbs has raised a worldwide concern. In this study, heavy metals in Epimedii Folium, an edible medicinal plant in China, were quantitatively analyzed. Variations of heavy metals in different species, in various organs (i.e., leaves, stems, and roots), in wild-growing and cultivated plants, and in 35 market samples of Epimedii Folium, were systematically investigated. In all of Epimedium samples, Hg (mercury) was not detectable (0.00 μg/g). Four species, Epimedium pubescens, Epimedium sagittatum, Epimedium brevicornu, and Epimedium wushanense, were found to contain Cu (copper) and Pb (lead). And contents of Cu and Pb in E. brevicornu were significantly higher than those in other species (P < 0.01). In wild-growing and cultivated Epimedium plants, Cd (cadmium) and As (arsenic) were not detectable, and concentrations of Cu and Pb in wild-growing plants were significantly higher than those in cultivated plants (P < 0.01). Cd was not detectable in leaves, roots, and stems, while organ specificity was apparent in the distribution of Cu, As, and Pb. And the highest levels of Cu and Pb were observed in roots and leaves, respectively. In Chinese markets, several samples of Epimedii Folium contained excessive Cu, Cd, As, and Pb beyond the national permissible limits. In summary, there was a large variation of heavy metals among Epimedii Folium samples, and Cu and Pb were the most important heavy metals contaminating the edible medicinal plant. Application of Epimedii Folium to drug and food industries will need to focus more on toxic heavy metal contamination.", "gemini": "Ô nhiễm kim loại nặng độc hại trong các loại thảo dược ăn được của Trung Quốc đã gây ra mối quan tâm trên toàn thế giới. Trong nghiên cứu này, kim loại nặng trong Epimedii Folium, một loại dược liệu ăn được ở Trung Quốc, đã được phân tích định lượng. Sự biến đổi của kim loại nặng ở các loài khác nhau, ở các cơ quan khác nhau (tức là lá, thân và rễ), ở cây mọc hoang và cây trồng, và trong 35 mẫu Epimedii Folium trên thị trường đã được nghiên cứu một cách có hệ thống. Trong tất cả các mẫu Epimedium, không phát hiện thấy Hg (thủy ngân) (0,00 μg/g). Bốn loài Epimedium pubescens, Epimedium sagittatum, Epimedium brevicornu và Epimedium wushanense được phát hiện có chứa Cu (đồng) và Pb (chì). Và hàm lượng Cu và Pb trong E. brevicornu cao hơn đáng kể so với các loài khác (P < 0,01). Ở cây Epimedium mọc hoang và trồng, không phát hiện thấy Cd (caдми) và As (asen), và nồng độ Cu và Pb ở cây mọc hoang cao hơn đáng kể so với cây trồng (P < 0,01). Không phát hiện thấy Cd trong lá, rễ và thân, trong khi tính đặc hiệu của cơ quan biểu hiện rõ ràng trong sự phân bố của Cu, As và Pb. Và nồng độ Cu và Pb cao nhất được quan sát thấy ở rễ và lá tương ứng. Trên thị trường Trung Quốc, một số mẫu Epimedii Folium có hàm lượng Cu, Cd, As và Pb vượt quá giới hạn cho phép của quốc gia. Tóm lại, có sự biến đổi lớn về kim loại nặng giữa các mẫu Epimedii Folium, và Cu và Pb là những kim loại nặng quan trọng nhất gây ô nhiễm cho loại dược liệu ăn được này. Việc ứng dụng Epimedii Folium vào ngành dược và thực phẩm sẽ cần tập trung nhiều hơn vào vấn đề ô nhiễm kim loại nặng độc hại."} +{"idx": 2000120, "en": "Provision of feed containing arsenic may cause intestinal flora imbalance and consequently, the dysfunction of immunological protection of the cock. To understand the intricate tuning of immune responses in the intestinal tract elicited by subchronic arsenism, a cock model (1-day-old Hy-line cocks) was established by subjecting cocks to different environmentally relevant concentrations of arsenic in the diet for 90 days. Intestinal cytokine and immunoglobulin (Ig) messenger RNA (mRNA) expression levels were determined using real-time PCR on days 30, 60, and 90. Results showed that, compared to those of the control groups, the mRNA levels of interleukin (IL)-1β, IL-2, IL-4, and interferon (IFN)-γ displayed increases on day 30 then decreases on days 60 and 90 dose-dependently in every tissue. Except for the decrease in the jejunum, the mRNA levels of IL-6 and IL-8 were increased in the duodenum, ileum, cecum, and rectum. However, the mRNA levels of IL-12β decreased in every tissue and every time point compared to those of the control groups. In contrast, chicks showed considerably higher expression levels of IgA, IgM, and IgG after exposure to arsenic. These results demonstrated that immune strategies of cocks were disturbed when suffered from subchronic arsenism, at least on the intestinal level.", "gemini": "Việc cung cấp thức ăn có chứa asen có thể gây mất cân bằng hệ vi khuẩn đường ruột và do đó, làm suy giảm chức năng bảo vệ miễn dịch của gà trống. Để hiểu được sự điều chỉnh phức tạp của phản ứng miễn dịch trong đường ruột do bệnh arsenism mạn tính gây ra, một mô hình gà trống (gà trống Hy-line 1 ngày tuổi) đã được thiết lập bằng cách cho gà trống tiếp xúc với các nồng độ asen khác nhau có liên quan đến môi trường trong thức ăn trong 90 ngày. Nồng độ biểu hiện RNA thông tin (mRNA) của cytokine đường ruột và immunoglobulin (Ig) được xác định bằng phản ứng PCR theo thời gian thực vào các ngày 30, 60 và 90. Kết quả cho thấy, so với nhóm đối chứng, nồng độ mRNA của interleukin (IL)-1β, IL-2, IL-4 và interferon (IFN)-γ tăng vào ngày 30 sau đó giảm vào các ngày 60 và 90 theo liều lượng phụ thuộc vào từng mô. Ngoại trừ sự giảm ở ruột non, nồng độ mRNA của IL-6 và IL-8 tăng ở tá tràng, hồi tràng, manh tràng và trực tràng. Tuy nhiên, nồng độ mRNA của IL-12β giảm ở mọi mô và mọi thời điểm so với nhóm đối chứng. Ngược lại, gà con biểu hiện nồng độ IgA, IgM và IgG cao hơn đáng kể sau khi tiếp xúc với asen. Những kết quả này chứng minh rằng các chiến lược miễn dịch của gà trống bị rối loạn khi bị bệnh arsenism mạn tính, ít nhất là ở mức độ đường ruột."} +{"idx": 2000121, "en": "Vaccines have been successful in reducing the mortality and morbidity, but most of them are delivered by intramuscular or intravenous route. They are associated with pain to the baby and bring lot of anxiety for the parents. There has been a marked increase in the number of injections required in first two years of life for completing the vaccination schedule. Hence, there is a need to have a painless vaccine delivery system. Numerous new routes of vaccination like, oral, nasal and transdermal routes are being tried. Oral polio and intranasal influenza have already been a success. Other newer approaches like edible vaccines, nasal sprays, dry powder preparations, jet injectors, microneedles and nanopatches are promising in delivering painless vaccines. Many of them are under clinical trials. These vaccine delivery systems will not only be painless but also cost effective, safe and easy to administer in mass population. They may be devoid of the need of cold chain. Painless delivery system will ensure better compliance to vaccination schedule.", "gemini": "Vắc-xin đã thành công trong việc giảm tỷ lệ tử vong và mắc bệnh, nhưng hầu hết chúng đều được đưa vào cơ thể bằng đường tiêm bắp hoặc tiêm tĩnh mạch. Chúng gây đau cho trẻ và mang lại nhiều lo lắng cho cha mẹ. Số lượng mũi tiêm cần thiết trong hai năm đầu đời để hoàn thành lịch tiêm chủng đã tăng đáng kể. Do đó, cần có hệ thống tiêm vắc-xin không đau. Nhiều đường tiêm vắc-xin mới như đường uống, đường mũi và đường thấm qua da đang được thử nghiệm. Vắc-xin bại liệt uống và vắc-xin cúm dạng xịt mũi đã thành công. Các phương pháp tiếp cận mới hơn khác như vắc-xin ăn được, thuốc xịt mũi, chế phẩm bột khô, máy tiêm phản lực, kim siêu nhỏ và miếng dán nano hứa hẹn trong việc cung cấp vắc-xin không đau. Nhiều loại trong số chúng đang được thử nghiệm lâm sàng. Các hệ thống tiêm vắc-xin này không chỉ không đau mà còn tiết kiệm chi phí, an toàn và dễ sử dụng cho đông đảo người dân. Chúng có thể không cần đến chuỗi lạnh. Hệ thống tiêm không đau sẽ đảm bảo tuân thủ tốt hơn lịch tiêm chủng."} +{"idx": 2000122, "en": "Retinoblastoma represents 3% of all childhood cancers, and is the most common intraocular malignancy of childhood. It is fatal, if untreated. White eye reflex, also known as leukocoria, is the commonest sign, followed by strabismus. The pediatricians have a very important role to play in the diagnosis of this relatively rare, but easily detectable tumor. Early diagnosis yields better results. The management of retinoblastoma has gradually evolved over the past few decades, with an aim to not only preserve life and eye, but also optimize residual vision. The treatment of retinoblastoma is multimodal, with chemotherapy, focal treatment including trans-pupillary thermotherapy, cryotherapy and laser photocoagulation, radiation therapy and surgery, all playing a vital role. Intravenous chemotherapy has been the mainstay of treatment for the past two decades, and still continues to be the most extensively used eye-saving modality of treatment. Periocular and intravitreal chemotherapy have specific indications in the management of retinoblastoma. Intra-arterial chemotherapy has emerged as a promising alternative for advanced and refractory retinoblastoma, both as a primary and secondary therapy. Recent advances in genetics of retinoblastoma have also helped in improving the overall clinical management of this malignancy.", "gemini": "U nguyên bào võng mạc chiếm 3% trong số tất cả các bệnh ung thư ở trẻ em và là loại u ác tính trong nhãn cầu phổ biến nhất ở trẻ em. Đây là căn bệnh gây tử vong nếu không được điều trị. Dấu hiệu phổ biến nhất là phản xạ mắt trắng, còn được gọi là bệnh bạch cầu, tiếp theo là lác mắt. Các bác sĩ nhi khoa có vai trò rất quan trọng trong việc chẩn đoán loại u tương đối hiếm nhưng dễ phát hiện này. Chẩn đoán sớm mang lại kết quả tốt hơn. Việc điều trị u nguyên bào võng mạc đã dần phát triển trong vài thập kỷ qua, với mục đích không chỉ bảo toàn tính mạng và mắt mà còn tối ưu hóa thị lực còn lại. Việc điều trị u nguyên bào võng mạc là đa phương thức, bao gồm hóa trị, điều trị tại chỗ bao gồm nhiệt trị xuyên đồng tử, liệu pháp lạnh và quang đông laser, xạ trị và phẫu thuật, tất cả đều đóng vai trò quan trọng. Hóa trị tĩnh mạch đã trở thành phương pháp điều trị chính trong hai thập kỷ qua và vẫn tiếp tục là phương pháp điều trị cứu mắt được sử dụng rộng rãi nhất. Hóa trị quanh mắt và nội dịch kính có những chỉ định cụ thể trong việc điều trị u nguyên bào võng mạc. Hóa trị nội động mạch đã nổi lên như một phương pháp thay thế đầy hứa hẹn cho u nguyên bào võng mạc tiến triển và kháng trị, cả như một liệu pháp chính và thứ phát. Những tiến bộ gần đây trong di truyền học của u nguyên bào võng mạc cũng đã giúp cải thiện việc quản lý lâm sàng tổng thể của bệnh ác tính này."} +{"idx": 2000123, "en": "Management of congenital diaphragmatic hernia (CDH) begins soon after it is detected, whether antenatally or postnatally. Assessment of the severity of the condition, associated congenital anomalies, maternal health and related issues, weight of the fetus/baby, mode of delivery, timing of delivery, immediate appropriate management of the baby with CDH at birth, appropriate utilization of available treatment modalities as well as infrastructure of the treating institute have an impact on the outcome of the neonate. Survival without significant long-term/permanent morbidity is considered as good outcome. With advances in antenatal diagnosis, several legal and ethical considerations have cropped up. While on one hand there are proponents of early antenatal diagnosis and medical termination of pregnancy (MTP), on the other hand there are several socio-cultural groups who look upon human life as precious and argue against MTP. There is an ongoing ethical battle between maternal vs. fetal rights; there is no way to put a lid on the controversy whether the mother be allowed to choose in favor of MTP after being aware of the anomalous fetus or, we must attempt to save every fetus irrespective of the antenatal diagnosis of life-threatening anomalies. Notwithstanding, appropriate assessment of the condition, thorough counseling and sound evidence-based decisions could avert ethical dilemma in most cases. This review article provides information about the various choices available in the diagnostic and treatment armamentarium, though it should be kept in mind that the entire spectrum of management strategies may not be universally available.", "gemini": "Việc xử trí thoát vị hoành bẩm (CDH) bắt đầu ngay sau khi phát hiện, bất kể là trước sinh hay sau sinh. Đánh giá mức độ nghiêm trọng của tình trạng, các dị tật bẩm sinh liên quan, sức khỏe của mẹ và các vấn đề liên quan, cân nặng của thai nhi/trẻ sơ sinh, phương pháp sinh, thời điểm sinh, xử trí thích hợp ngay lập tức cho trẻ sơ sinh bị CDH khi sinh, sử dụng thích hợp các phương thức điều trị có sẵn cũng như cơ sở hạ tầng của cơ sở điều trị có tác động đến kết quả của trẻ sơ sinh. Sống sót mà không có tình trạng bệnh tật lâu dài/vĩnh viễn đáng kể được coi là kết quả tốt. Với sự tiến bộ trong chẩn đoán trước sinh, một số cân nhắc về mặt pháp lý và đạo đức đã nảy sinh. Trong khi một mặt có những người ủng hộ chẩn đoán trước sinh sớm và chấm dứt thai kỳ (MTP), mặt khác có một số nhóm xã hội-văn hóa coi trọng sự sống của con người và phản đối MTP. Có một cuộc chiến về đạo đức đang diễn ra giữa quyền của mẹ so với quyền của thai nhi; không có cách nào để chấm dứt tranh cãi về việc liệu người mẹ có được phép lựa chọn ủng hộ MTP sau khi biết về thai nhi bất thường hay không, hoặc chúng ta phải cố gắng cứu mọi thai nhi bất kể chẩn đoán trước sinh về các dị tật đe dọa tính mạng. Tuy nhiên, việc đánh giá đúng tình trạng, tư vấn kỹ lưỡng và các quyết định dựa trên bằng chứng hợp lý có thể tránh được tình trạng tiến thoái lưỡng nan về mặt đạo đức trong hầu hết các trường hợp. Bài tổng quan này cung cấp thông tin về các lựa chọn khác nhau có sẵn trong chẩn đoán và điều trị, mặc dù cần lưu ý rằng toàn bộ các chiến lược quản lý có thể không có sẵn ở khắp mọi nơi."} +{"idx": 2000124, "en": "The term meningocele manqué (MM) was coined in 1972 to describe a broad range of surgical findings characterized by intradural bands tethering neural structures to the dorsal dura. Over the following decades, reports continued to lump intradural tethering bands associated with a variety of comorbidities under the umbrella term MM. In more recent years, disorders previously called MM have been identified as embryologically distinct and were reclassified. While this sectioning continues, there remains a set of intradural tethering disorders for which no better term than MM exists. Herein, we comprehensively review the literature surrounding MM, including embryological disorders, clinical, radiographic, and surgical presentation, as well as alternative classification methods and MM treatment.", "gemini": "Thuật ngữ meningocele manqué (MM) được đặt ra vào năm 1972 để mô tả một loạt các phát hiện phẫu thuật đặc trưng bởi các dải nội sọ cột chặt các cấu trúc thần kinh vào màng cứng lưng. Trong những thập kỷ tiếp theo, các báo cáo tiếp tục tập hợp các dải cột chặt nội sọ liên quan đến nhiều bệnh đi kèm dưới thuật ngữ chung là MM. Trong những năm gần đây, các rối loạn trước đây được gọi là MM đã được xác định là khác biệt về mặt phôi thai và được phân loại lại. Trong khi việc phân loại này vẫn tiếp tục, vẫn còn một nhóm các rối loạn cột chặt nội sọ mà không có thuật ngữ nào tốt hơn MM. Trong bài viết này, chúng tôi xem xét toàn diện các tài liệu xung quanh MM, bao gồm các rối loạn phôi thai, biểu hiện lâm sàng, hình ảnh học và phẫu thuật, cũng như các phương pháp phân loại thay thế và điều trị MM."} +{"idx": 2000125, "en": "The objective of this study was to assess the prostate cancer screening practices of Vermont primary care physicians and compare them with a prior study in 2001. An electronic survey was created and emailed to all currently practicing primary care physicians in Vermont. Data was stratified by practice length, practice location, university affiliation, and internal medicine versus family practice. Surveys were received from 123 (27.2%) primary care physicians. 27.7% of physicians in practice <10 years recommended prostate specific antigen (PSA) testing, compared with 55.9% of those practicing ≥10 years (p = 0.006). Of those who modified their recommendations in the past 5 years, 96.1% reported that the United States Preventive Services Task Force (USPSTF) 2012 statement influenced them. Respondents who continued to use PSA testing were less likely to stop screening after age 80 compared with those surveyed in 2001 (51% in 2014 vs. 74% in 2001; p <0.001). Primary care physicians in practice for 10 or more years were more likely to recommend PSA-based screening than those in practice for less time. The USPSTF statement discouraging PSA-based screening for prostate cancer has had significant penetrance among Vermont primary care physicians.", "gemini": "Mục tiêu của nghiên cứu này là đánh giá các hoạt động sàng lọc ung thư tuyến tiền liệt của các bác sĩ chăm sóc chính tại Vermont và so sánh chúng với một nghiên cứu trước đó vào năm 2001. Một cuộc khảo sát điện tử đã được tạo và gửi qua email cho tất cả các bác sĩ chăm sóc chính hiện đang hành nghề tại Vermont. Dữ liệu được phân tầng theo thời gian hành nghề, địa điểm hành nghề, liên kết với trường đại học và nội khoa so với hành nghề gia đình. Các cuộc khảo sát đã nhận được từ 123 (27,2%) bác sĩ chăm sóc chính. 27,7% bác sĩ hành nghề <10 năm khuyến nghị xét nghiệm kháng nguyên đặc hiệu tuyến tiền liệt (PSA), so với 55,9% bác sĩ hành nghề ≥10 năm (p = 0,006). Trong số những người đã sửa đổi khuyến nghị của mình trong 5 năm qua, 96,1% báo cáo rằng tuyên bố năm 2012 của Lực lượng đặc nhiệm dịch vụ phòng ngừa Hoa Kỳ (USPSTF) đã ảnh hưởng đến họ. Những người trả lời tiếp tục sử dụng xét nghiệm PSA ít có khả năng ngừng sàng lọc sau 80 tuổi so với những người được khảo sát vào năm 2001 (51% vào năm 2014 so với 74% vào năm 2001; p <0,001). Các bác sĩ chăm sóc chính hành nghề trong 10 năm trở lên có nhiều khả năng khuyến nghị sàng lọc dựa trên PSA hơn những người hành nghề trong thời gian ngắn hơn. Tuyên bố của USPSTF khuyến cáo không nên sàng lọc dựa trên PSA đối với ung thư tuyến tiền liệt đã có sự thâm nhập đáng kể trong số các bác sĩ chăm sóc chính tại Vermont."} +{"idx": 2000126, "en": "Chronic dietary exposure to the cyanobacterial toxin β-N-methylamino-L-alanine (BMAA) triggers neuropathology in non-human primates, providing support for the theory that BMAA causes a fatal neurodegenerative illness among the indigenous Chamorro people of Guam. However, since there are two stereoisomers of BMAA, it is important to know if both can occur in nature, and if so, what role they might play in disease causation. As a first step, we analysed both BMAA enantiomers in cyanobacteria, cycads, and in mammals orally dosed with L-BMAA, to determine if enantiomeric changes could occur in vivo. BMAA in cyanobacteria and cycads was found only as the L-enantiomer. However, while the L-enantiomer in mammals was little changed after digestion, we detected a small pool of D-BMAA in the liver (12.5%) of mice and in the blood plasma of vervets (3.6%). Chiral analysis of cerebrospinal fluid of vervets and hindbrain of mice showed that the free BMAA in the central nervous system was the D-enantiomer. In vitro toxicity investigations with D-BMAA showed toxicity, mediated through AMPA rather than NMDA receptors. These findings raise important considerations concerning the neurotoxicity of BMAA and its relationship to neurodegenerative disease.", "gemini": "Tiếp xúc lâu dài với độc tố vi khuẩn lam β-N-methylamino-L-alanine (BMAA) trong chế độ ăn uống gây ra bệnh lý thần kinh ở các loài linh trưởng không phải người, cung cấp bằng chứng cho thấy BMAA gây ra một căn bệnh thoái hóa thần kinh gây tử vong ở người dân bản địa Chamorro ở Guam. Tuy nhiên, vì có hai đồng phân lập thể của BMAA nên điều quan trọng là phải biết liệu cả hai đều có thể xảy ra trong tự nhiên hay không và nếu có, chúng có thể đóng vai trò gì trong việc gây bệnh. Bước đầu tiên, chúng tôi đã phân tích cả hai đồng phân quang học BMAA trong vi khuẩn lam, cây họ tuế và ở động vật có vú được uống L-BMAA để xác định xem sự thay đổi đồng phân quang học có thể xảy ra trong cơ thể sống hay không. BMAA trong vi khuẩn lam và cây họ tuế chỉ được tìm thấy ở dạng đồng phân L. Tuy nhiên, trong khi đồng phân L ở động vật có vú hầu như không thay đổi sau khi tiêu hóa, chúng tôi phát hiện ra một lượng nhỏ D-BMAA trong gan (12,5%) của chuột và trong huyết tương của khỉ vervet (3,6%). Phân tích độ quang học của dịch não tủy của khỉ vervet và não sau của chuột cho thấy BMAA tự do trong hệ thần kinh trung ương là đồng phân D. Các nghiên cứu độc tính trong ống nghiệm với D-BMAA cho thấy độc tính, được trung gian thông qua thụ thể AMPA chứ không phải thụ thể NMDA. Những phát hiện này nêu lên những cân nhắc quan trọng liên quan đến độc tính của BMAA đối với thần kinh và mối quan hệ của nó với bệnh thoái hóa thần kinh."} +{"idx": 2000127, "en": "Life history theory predicts a trade-off between male sexual trait expression and immunocompetence. Using burying beetles, Nicrophorus vespilloides, as a model, we investigated the relationship between male immune function, sex pheromone emission, and attractiveness under field conditions. In the first experiment, we tested whether there is a positive correlation between immune capacity, sex pheromone characteristics (quantity, relative composition, and time invested in pheromone emission), and male attractiveness. As a measurement of immune capacity, we used an individual's encapsulation ability against a novel antigen. In the second experiment, we specifically examined whether a trade-off between chemical trait expression and immune function existed. To this end, we challenged the immune system and measured the subsequent investment in sex pheromone emission and the attractiveness of the male under field conditions. We found that a male's immunocompetence was neither related to the emission of the male's sex pheromone nor to its attractiveness in the field. Furthermore, none of the immune-challenge treatments affected the subsequent investment in pheromone emission or number of females attracted. However, we showed that the same males that emitted a high quantity of their sex pheromone in the laboratory were able to attract more females in the field. Our data suggest that the chemical signal is not a reliable predictor of a male's immunocompetence but rather is a general important fitness-related trait, with a higher emission of the sex pheromone measured in the laboratory directly affecting the attractiveness of a male under field conditions.", "gemini": "Lý thuyết lịch sử cuộc sống dự đoán sự đánh đổi giữa biểu hiện đặc điểm tình dục của nam giới và khả năng miễn dịch. Sử dụng bọ cánh cứng chôn cất, Nicrophorus vespilloides, làm mô hình, chúng tôi đã nghiên cứu mối quan hệ giữa chức năng miễn dịch của nam giới, phát xạ pheromone tình dục và sức hấp dẫn trong điều kiện thực tế. Trong thí nghiệm đầu tiên, chúng tôi đã kiểm tra xem có mối tương quan tích cực giữa khả năng miễn dịch, đặc điểm pheromone tình dục (số lượng, thành phần tương đối và thời gian đầu tư vào phát xạ pheromone) và sức hấp dẫn của nam giới hay không. Là phép đo khả năng miễn dịch, chúng tôi đã sử dụng khả năng đóng nang của một cá thể chống lại một kháng nguyên mới. Trong thí nghiệm thứ hai, chúng tôi đã kiểm tra cụ thể xem có sự đánh đổi giữa biểu hiện đặc điểm hóa học và chức năng miễn dịch hay không. Để đạt được mục đích này, chúng tôi đã thử thách hệ thống miễn dịch và đo lường sự đầu tư tiếp theo vào phát xạ pheromone tình dục và sức hấp dẫn của con đực trong điều kiện thực tế. Chúng tôi thấy rằng khả năng miễn dịch của một con đực không liên quan đến việc phát ra pheromone tình dục của con đực cũng như sức hấp dẫn của nó trong thực tế. Hơn nữa, không có phương pháp điều trị thử thách miễn dịch nào ảnh hưởng đến khoản đầu tư tiếp theo vào phát xạ pheromone hoặc số lượng con cái bị thu hút. Tuy nhiên, chúng tôi đã chỉ ra rằng những con đực phát ra lượng pheromone tình dục cao trong phòng thí nghiệm có thể thu hút nhiều con cái hơn ngoài thực tế. Dữ liệu của chúng tôi cho thấy rằng tín hiệu hóa học không phải là một yếu tố dự đoán đáng tin cậy về khả năng miễn dịch của một con đực mà đúng hơn là một đặc điểm quan trọng liên quan đến thể lực, với lượng pheromone tình dục cao hơn được đo trong phòng thí nghiệm ảnh hưởng trực tiếp đến sức hấp dẫn của một con đực trong điều kiện thực tế."} +{"idx": 2000128, "en": "The purpose of this review article is to summarize the views presented in the Hippocratic Corpus of Medicine regarding injuries of the spine, their effect on the spinal cord and the optimal treatment modulus. It is shown that the treatment dilemmas of ancient times in these injuries remain modern since Hippocrates suggested that conservative treatment and letting nature take its course is preferable over a devastating surgical treatment if the spinal cord structural integrity is not compromised. There is also a detailed account of the conservative treatment suggested in the Hippocratic Corpus concerning devices and method of closed spinal relocations.", "gemini": "Mục đích của bài tổng quan này là tóm tắt các quan điểm được trình bày trong Tập hợp Y học Hippocrates về chấn thương cột sống, tác động của chúng lên tủy sống và mô-đun điều trị tối ưu. Điều này cho thấy rằng những khó khăn trong điều trị thời cổ đại đối với những chấn thương này vẫn còn hiện đại vì Hippocrates cho rằng điều trị bảo tồn và để tự nhiên diễn biến là tốt hơn so với phẫu thuật tàn phá nếu tính toàn vẹn về cấu trúc của tủy sống không bị tổn hại. Ngoài ra còn có một mô tả chi tiết về phương pháp điều trị bảo tồn được đề xuất trong Tập hợp Hippocrates liên quan đến các thiết bị và phương pháp phục hồi cột sống kín."} +{"idx": 2000129, "en": "E-cadherin is a transmembrane glycoprotein that facilitates inter-cellular adhesion in the epithelium. The ectodomain of the native structure is comprised of five repeated immunoglobulin-like domains. All E-cadherin crystal structures show the protein in one of three alternative conformations: a monomer, a strand-swapped trans homodimer and the so-called X-dimer, which is proposed to be a kinetic intermediate to forming the strand-swapped trans homodimer. However, previous studies have indicated that even once the trans strand-swapped dimer is formed, the complex is highly dynamic and the E-cadherin monomers may reorient relative to each other. Here, molecular dynamics simulations have been used to investigate the stability and conformational flexibility of the human E-cadherin trans strand-swapped dimer. In four independent, 100 ns simulations, the dimer moved away from the starting structure and converged to a previously unreported structure, which we call the Y-dimer. The Y-dimer was present for over 90% of the combined simulation time, suggesting that it represents a stable conformation of the E-cadherin dimer in solution. The Y-dimer conformation is stabilised by interactions present in both the trans strand-swapped dimer and X-dimer crystal structures, as well as additional interactions not found in any E-cadherin dimer crystal structures. The Y-dimer represents a previously unreported, stable conformation of the human E-cadherin trans strand-swapped dimer and suggests that the available crystal structures do not fully capture the conformations that the human E-cadherin trans homodimer adopts in solution.", "gemini": "E-cadherin là một glycoprotein xuyên màng tạo điều kiện cho sự kết dính giữa các tế bào trong biểu mô. Ngoại miền của cấu trúc bản địa bao gồm năm miền giống immunoglobulin được lặp lại. Tất cả các cấu trúc tinh thể của E-cadherin đều cho thấy protein ở một trong ba dạng thay thế: một thể đơn phân, một thể đồng hợp tử chuyển đổi sợi và cái gọi là X-dimer, được đề xuất là một chất trung gian động học để tạo thành thể đồng hợp tử chuyển đổi sợi. Tuy nhiên, các nghiên cứu trước đây đã chỉ ra rằng ngay cả khi thể dị hợp tử chuyển đổi sợi được hình thành, phức hợp vẫn có tính động cao và các đơn phân E-cadherin có thể định hướng lại so với nhau. Ở đây, mô phỏng động học phân tử đã được sử dụng để nghiên cứu tính ổn định và tính linh hoạt về mặt cấu hình của thể dị hợp tử chuyển đổi sợi E-cadherin của người. Trong bốn mô phỏng độc lập, 100 ns, thể dị hợp tử di chuyển ra khỏi cấu trúc ban đầu và hội tụ thành một cấu trúc chưa từng được báo cáo trước đây, mà chúng tôi gọi là thể Y. Thể Y có mặt trong hơn 90% thời gian mô phỏng kết hợp, cho thấy rằng nó đại diện cho một cấu hình ổn định của thể dị hợp tử E-cadherin trong dung dịch. Cấu hình thể Y được ổn định bởi các tương tác có trong cả thể dị hợp tử chuyển đổi sợi và cấu trúc tinh thể X-dimer, cũng như các tương tác bổ sung không tìm thấy trong bất kỳ cấu trúc tinh thể thể dị hợp tử E-cadherin nào. Thể Y đại diện cho một cấu hình ổn định, chưa từng được báo cáo trước đây của thể dị hợp tử chuyển đổi sợi E-cadherin của người và cho thấy rằng các cấu trúc tinh thể có sẵn không nắm bắt đầy đủ các cấu hình mà thể đồng hợp tử chuyển đổi sợi E-cadherin của người áp dụng trong dung dịch."} +{"idx": 2000130, "en": "The propagation, or movement, of a conformational kink along a linear structure can occur in capture, transfer, or multireflection modes. Here we use a model for two-component bistable polymer molecules with energetically non-equivalent stable states to model the propagation of the DNA transcription bubble via movement of a non-linear longitudinal stretching region along the DNA chain. We show that under certain conditions the longitudinal excitation can act as a reflector for a conformational kink and, furthermore, that conformational switching may propagate in a multireflection mode alongside conformational kink transition and conformational kink trapping.", "gemini": "Sự lan truyền hoặc chuyển động của một nếp gấp cấu hình dọc theo một cấu trúc tuyến tính có thể xảy ra ở chế độ bắt giữ, truyền hoặc phản xạ nhiều lần. Ở đây, chúng tôi sử dụng một mô hình cho các phân tử polyme bistable hai thành phần với các trạng thái ổn định không tương đương về mặt năng lượng để mô hình hóa sự lan truyền của bong bóng phiên mã DNA thông qua chuyển động của một vùng kéo dài theo chiều dọc phi tuyến tính dọc theo chuỗi DNA. Chúng tôi chỉ ra rằng trong một số điều kiện nhất định, sự kích thích theo chiều dọc có thể đóng vai trò như một bộ phản xạ cho một nếp gấp cấu hình và hơn nữa, sự chuyển đổi cấu hình có thể lan truyền theo chế độ phản xạ nhiều lần cùng với quá trình chuyển đổi nếp gấp cấu hình và giữ nếp gấp cấu hình."} +{"idx": 2000131, "en": "Interactions of carbon nanotubes (CNTs) and blood proteins are of interest for nanotoxicology and nanomedicine. It is believed that the interactions of blood proteins and glycoproteins with CNTs may have important biological effects. In spite of many experimental studies of single-walled carbon nanotubes (SWCNT) and glycoproteins with different methods, little is known about the atomistic details of their association process or of structural alterations occurring in adsorbed glycoproteins. In this study, we have applied molecular dynamics simulation to investigate the interaction of follicle stimulating hormone (hFSH) with SWCNT. The aim of this work is to investigate possible mechanisms of nanotoxicity at a molecular level. We present details of the molecular dynamics, structure, and free energy of binding of hFSH on the surface of SWCNT. We find that hFSH in aqueous solution strongly adsorbs onto SWCNT via their concave surface as evidenced by high binding free energies for residues in both protein subunits. It was found that hydrophobic, π-cation, and π-π stacking interactions are the main driving forces for the adsorption of the protein at the nanotube surface.", "gemini": "Tương tác của các ống nano cacbon (CNT) và protein máu rất được quan tâm trong lĩnh vực độc chất nano và y học nano. Người ta tin rằng các tương tác của protein máu và glycoprotein với CNT có thể có những ảnh hưởng sinh học quan trọng. Mặc dù có nhiều nghiên cứu thực nghiệm về ống nano cacbon thành vách đơn (SWCNT) và glycoprotein bằng các phương pháp khác nhau, nhưng người ta vẫn biết rất ít về các chi tiết nguyên tử của quá trình liên kết hoặc các thay đổi về cấu trúc xảy ra ở các glycoprotein được hấp phụ. Trong nghiên cứu này, chúng tôi đã áp dụng mô phỏng động học phân tử để nghiên cứu sự tương tác của hormone kích thích nang trứng (hFSH) với SWCNT. Mục đích của công trình này là nghiên cứu các cơ chế có thể xảy ra của độc tính nano ở cấp độ phân tử. Chúng tôi trình bày chi tiết về động học phân tử, cấu trúc và năng lượng tự do liên kết của hFSH trên bề mặt SWCNT. Chúng tôi thấy rằng hFSH trong dung dịch nước hấp phụ mạnh vào SWCNT thông qua bề mặt lõm của chúng, bằng chứng là năng lượng tự do liên kết cao đối với các phần tử trong cả hai tiểu đơn vị protein. Người ta thấy rằng các tương tác xếp chồng kỵ nước, π-cation và π-π là những lực thúc đẩy chính cho sự hấp phụ của protein tại bề mặt ống nano."} +{"idx": 2000132, "en": "The objective assessments of left ventricular (LV) and right ventricular (RV) ejection fractions (EFs) are the main important tasks of routine cardiovascular magnetic resonance (CMR). Over the years, CMR has emerged as the reference standard for the evaluation of biventricular morphology and function. However, changes in EF may occur in the late stages of the majority of cardiac diseases, and being a measure of global function, it has limited sensitivity for identifying regional myocardial impairment. On the other hand, current wall motion evaluation is done on a subjective basis and subjective, qualitative analysis has a substantial error rate. In an attempt to better quantify global and regional LV function; several techniques, to assess myocardial deformation, have been developed, over the past years. The aim of this review is to provide a comprehensive compendium of all the CMR techniques to assess myocardial deformation parameters as well as the application in different clinical scenarios.", "gemini": "Các đánh giá khách quan về phân suất tống máu thất trái (LV) và thất phải (RV) (EF) là nhiệm vụ quan trọng chính của cộng hưởng từ tim mạch thường quy (CMR). Trong nhiều năm qua, CMR đã nổi lên như tiêu chuẩn tham chiếu để đánh giá hình thái và chức năng của cả hai tâm thất. Tuy nhiên, những thay đổi về EF có thể xảy ra ở giai đoạn muộn của hầu hết các bệnh tim và là một biện pháp đánh giá chức năng toàn cầu, nó có độ nhạy hạn chế để xác định suy giảm cơ tim khu vực. Mặt khác, đánh giá chuyển động thành tim hiện tại được thực hiện trên cơ sở chủ quan và phân tích định tính, chủ quan có tỷ lệ lỗi đáng kể. Trong nỗ lực định lượng tốt hơn chức năng LV toàn cầu và khu vực; một số kỹ thuật để đánh giá biến dạng cơ tim đã được phát triển trong những năm qua. Mục đích của bài đánh giá này là cung cấp một bản tóm tắt toàn diện về tất cả các kỹ thuật CMR để đánh giá các thông số biến dạng cơ tim cũng như ứng dụng trong các tình huống lâm sàng khác nhau."} +{"idx": 2000133, "en": "BACKGROUND Meckel-Gruber syndrome (MKS, OMIM #607361) is a rare pre- or perinatal lethal autosomal recessive ciliopathy caused by mutations in at least 12 known genes. It has a clinical and genetic overlap with other viable ciliopathies, especially Joubert syndrome and Joubert syndrome-related disorders. MKS is characterized by multicystic kidney dysplasia, central nervous system malformations (usually occipital encephalocele), ductal plate malformation of the liver, and postaxial polydactyly. CASE DIAGNOSIS We identified a homozygous mutation in TMEM67 (MKS3) in a fetus affected by MKS; however, only the mother was a carrier of the respective mutation. Genotyping with polymorphic microsatellite markers and single nucleotide polymorphism (SNP) array revealed a maternal uniparental disomy (UPD) of the entire chromosome 8 (upd(8)mat), harboring TMEM67. CONCLUSIONS This is the first reported case of UPD as a cause of MKS. The possible underlying mechanisms for uniparental disomy (UPD) are reviewed. Even if rare, awareness of UPD and comprehensive work-up in the case of unexpected homozygosity for a recessive mutation is essential for accurate genetic counseling and assessment of the risk of recurrence.", "gemini": "BỐI CẢNH Hội chứng Meckel-Gruber (MKS, OMIM # 607361) là một bệnh lý lông mao lặn trên nhiễm sắc thể thường gây tử vong trước hoặc trong thời kỳ chuyển dạ hiếm gặp do đột biến ở ít nhất 12 gen đã biết. Nó có sự chồng chéo về mặt lâm sàng và di truyền với các bệnh lý lông mao khác có thể sống được, đặc biệt là hội chứng Joubert và các rối loạn liên quan đến hội chứng Joubert. MKS được đặc trưng bởi loạn sản thận đa nang, dị dạng hệ thần kinh trung ương (thường là thoát vị não chẩm), dị dạng mảng ống của gan và đa ngón sau trục. CHẨN ĐOÁN TRƯỜNG HỢP Chúng tôi đã xác định được một đột biến đồng hợp tử ở TMEM67 (MKS3) ở một thai nhi bị ảnh hưởng bởi MKS; tuy nhiên, chỉ có người mẹ là người mang đột biến tương ứng. Phân tích kiểu gen với các dấu hiệu đa hình vi vệ tinh và mảng đơn nucleotide đa hình (SNP) cho thấy một nhiễm sắc thể đơn bội của mẹ (UPD) của toàn bộ nhiễm sắc thể 8 (upd (8) mat), chứa TMEM67. KẾT LUẬN Đây là trường hợp được báo cáo đầu tiên về UPD là nguyên nhân gây ra MKS. Các cơ chế tiềm ẩn có thể xảy ra đối với nhiễm sắc thể đơn bội (UPD) được xem xét. Mặc dù hiếm gặp, việc nhận thức về UPD và quá trình kiểm tra toàn diện trong trường hợp đồng hợp tử bất ngờ đối với một đột biến lặn là rất cần thiết để tư vấn di truyền chính xác và đánh giá nguy cơ tái phát."} +{"idx": 2000134, "en": "Amniotic fluid (AF) contains a heterogeneous population of cells that have been identified to possess pluripotent and progenitor-like characteristics. These cells have been applied in various regenerative medicine applications ranging from in vitro cell differentiation to tissue engineering to cellular therapies for different organs including the heart, the liver, the lung, and the kidneys. In this review, we examine the different methodologies used for the derivation of amniotic fluid stem cells and renal progenitors, and their application in renal repair and regeneration. Moreover, we discuss the recent achievements and newly emerging challenges in our understanding of their biology, their immunoregulatory characteristics, and their paracrine-mediated therapeutic potential for the treatment of acute and chronic kidney diseases.", "gemini": "Dịch ối (AF) chứa một quần thể tế bào không đồng nhất được xác định là có các đặc điểm giống như đa năng và tiền thân. Các tế bào này đã được ứng dụng trong nhiều ứng dụng y học tái tạo khác nhau, từ biệt hóa tế bào trong ống nghiệm đến kỹ thuật mô đến liệu pháp tế bào cho các cơ quan khác nhau bao gồm tim, gan, phổi và thận. Trong bài đánh giá này, chúng tôi xem xét các phương pháp khác nhau được sử dụng để lấy tế bào gốc dịch ối và tiền thân thận, và ứng dụng của chúng trong sửa chữa và tái tạo thận. Hơn nữa, chúng tôi thảo luận về những thành tựu gần đây và những thách thức mới nổi trong việc hiểu biết về sinh học của chúng, các đặc điểm điều hòa miễn dịch của chúng và tiềm năng điều trị thông qua trung gian cận tiết của chúng đối với việc điều trị các bệnh thận cấp tính và mãn tính."} +{"idx": 2000135, "en": "Richness and abundance of facultative symbionts vary strongly with aphid species and genotype, symbiont strain, host plant, biogeography, and a number of abiotic factors. Despite indications that aphids in the same ecological niche show similar levels of facultative symbiont richness, existing reports do not consider the potential role of host plants on aphid microbial community. Little is known about how oligophagy and polyphagy may be influenced by secondary symbiont distribution, mainly because studies on secondary symbiont diversity are biased towards polyphagous aphids from the Northern Hemisphere. Here, we demonstrate the richness and abundance of the most common aphid-associated facultative symbionts in two tropical aphid species, the oligophagous Aphis (Toxoptera) citricidus (Kirkaldy) (Hemiptera: Aphididae) and the polyphagous Aphis aurantii (Boyer de Fonscolombe) (Hemiptera: Aphididae). Aphis citricidus is restricted to Citrus sp. host plants and closely related genera, whereas A. aurantii successfully exploits a wide variety of host plants from different families. Both were collected in the same ecological niche and our data basically indicated the same richness of secondary symbionts, but the abundance at which secondary symbionts occurred was very distinct between the two species. Spiroplasma was the most abundant facultative symbiont associated with A. citricidus and A. aurantii in the ecological niche studied. Single and multiple secondary symbiont infections were observed, but diversity of multiple infections was particularly high in A. citricidus. We discuss our findings and suggest hypotheses to explain causes and consequences of the differences in secondary symbiont diversity observed between these two aphid species.", "gemini": "Sự phong phú và dồi dào của các cộng sinh tùy ý thay đổi mạnh mẽ tùy theo loài rệp và kiểu gen, chủng cộng sinh, cây chủ, địa sinh học và một số yếu tố phi sinh học. Mặc dù có những dấu hiệu cho thấy rệp trong cùng một hốc sinh thái cho thấy mức độ phong phú của cộng sinh tùy ý tương tự nhau, nhưng các báo cáo hiện có không xem xét vai trò tiềm tàng của cây chủ đối với cộng đồng vi sinh của rệp. Người ta biết rất ít về cách ăn ít và ăn nhiều có thể bị ảnh hưởng bởi sự phân bố của cộng sinh thứ cấp, chủ yếu là do các nghiên cứu về sự đa dạng của cộng sinh thứ cấp có xu hướng thiên về các loài rệp ăn nhiều ở Bán cầu Bắc. Tại đây, chúng tôi chứng minh sự phong phú và dồi dào của các cộng sinh tùy ý phổ biến nhất liên quan đến rệp ở hai loài rệp nhiệt đới, loài rệp ăn ít Aphis (Toxoptera) citricidus (Kirkaldy) (Hemiptera: Aphididae) và loài rệp ăn nhiều Aphis aurantii (Boyer de Fonscolombe) (Hemiptera: Aphididae). Aphis citricidus chỉ giới hạn ở cây chủ Citrus sp. và các chi có họ hàng gần, trong khi A. aurantii khai thác thành công nhiều loại cây chủ khác nhau từ các họ khác nhau. Cả hai đều được thu thập trong cùng một hốc sinh thái và dữ liệu của chúng tôi về cơ bản chỉ ra cùng mức độ phong phú của cộng sinh thứ cấp, nhưng mức độ dồi dào mà cộng sinh thứ cấp xuất hiện rất khác biệt giữa hai loài. Spiroplasma là cộng sinh tùy ý dồi dào nhất liên quan đến A. citricidus và A. aurantii trong hốc sinh thái được nghiên cứu. Nhiễm trùng cộng sinh thứ cấp đơn lẻ và nhiều lần đã được quan sát thấy, nhưng sự đa dạng của các nhiễm trùng nhiều lần đặc biệt cao ở A. citricidus. Chúng tôi thảo luận về những phát hiện của mình và đưa ra các giả thuyết để giải thích nguyên nhân và hậu quả của sự khác biệt về sự đa dạng của cộng sinh thứ cấp được quan sát thấy giữa hai loài rệp này."} +{"idx": 2000136, "en": "INTRODUCTION Colorectal surgeries (CRS) have one of the highest rates of surgical site infections (SSIs) with rates 15 to >30%. Prevention \"bundles\" or sets of evidence-based interventions are structured ways to improve patient outcomes. The aim sof this study is to evaluate CRS SSI prevention bundles, bundle components, and implementation and compliance strategies. METHODS A meta-analysis of studies with pre- and post-implementation data was conducted to assess the impact of bundles on SSI rates (superficial, deep, and organ/space). Subgroup analysis of bundle components identified optimal bundle designs. RESULTS Thirty-five studies (51,413 patients) were identified and 23 (17,557 patients) were included in the meta-analysis. A SSI risk reduction of 40% (p < 0.001) was noted with 44% for superficial SSI (p < 0.001) and 34% for organ/space (p = 0.048). Bundles with sterile closure trays (58.6 vs 33.1%), MBP with oral antibiotics (55.4 vs 31.8%), and pre-closure glove changes (56.9 vs 28.5%) had significantly greater SSI risk reduction. CONCLUSION Bundles can effectively reduce the risk of SSIs after CRS, by fostering a cohesive environment, standardization, and reduction in operative variance. If implemented successfully and complied with, bundles can become vital to improving patients' surgical quality of care.", "gemini": "GIỚI THIỆU Các cuộc phẫu thuật trực tràng (CRS) có tỷ lệ nhiễm trùng tại vị trí phẫu thuật (SSI) cao nhất với tỷ lệ từ 15 đến> 30%. Các \"gói\" phòng ngừa hoặc các nhóm can thiệp dựa trên bằng chứng là những cách có cấu trúc để cải thiện kết quả cho bệnh nhân. Mục đích của nghiên cứu này là đánh giá các gói phòng ngừa CRS SSI, các thành phần của gói và các chiến lược triển khai và tuân thủ. PHƯƠNG PHÁP Một phân tích tổng hợp các nghiên cứu có dữ liệu trước và sau khi triển khai đã được tiến hành để đánh giá tác động của các gói đối với tỷ lệ SSI (bề ngoài, sâu và cơ quan/khoang). Phân tích nhóm con của các thành phần gói đã xác định thiết kế gói tối ưu. KẾT QUẢ Ba mươi lăm nghiên cứu (51.413 bệnh nhân) đã được xác định và 23 (17.557 bệnh nhân) được đưa vào phân tích tổng hợp. Giảm nguy cơ SSI 40% (p <0,001) được ghi nhận với 44% đối với SSI nông (p <0,001) và 34% đối với cơ quan/khoang (p = 0,048). Các gói có khay đóng vô trùng (58,6 so với 33,1%), MBP với kháng sinh đường uống (55,4 so với 31,8%) và thay găng tay trước khi đóng (56,9 so với 28,5%) có khả năng giảm nguy cơ SSI đáng kể. KẾT LUẬN Các gói có thể làm giảm hiệu quả nguy cơ SSI sau CRS bằng cách thúc đẩy môi trường gắn kết, chuẩn hóa và giảm sự thay đổi trong quá trình phẫu thuật. Nếu được triển khai thành công và tuân thủ, các gói có thể trở nên quan trọng để cải thiện chất lượng chăm sóc phẫu thuật của bệnh nhân."} +{"idx": 2000137, "en": "PURPOSE AND OBJECTIVE To test the hypothesis that a rectal and bladder preparation protocol is associated with an increase in prostate cancer specific survival (PCSS), clinical disease free survival (CDFS) and biochemical disease free survival (BDFS). PATIENTS AND METHODS From 1999 to 2012, 1080 prostate cancer (PCa) patients were treated with three-dimensional conformal radiotherapy (3DCRT). Of these patients, 761 were treated with an empty rectum and comfortably full bladder (RBP) preparation protocol, while for 319 patients no rectal/bladder preparation (NRBP) protocol was adopted. RESULTS Compared with NRBP patients, patients with RBP had significantly higher BDFS (64% vs 48% at 10 years, respectively), CDFS (81% vs 70.5% at 10 years, respectively) and PCSS (95% vs 88% at 10 years, respectively) (log-rank test p < 0.001). Multivariate analysis (MVA) indicated for all treated patients and intermediate high-risk patients that the Gleason score (GS) and the rectal and bladder preparation were the most important prognostic factors for PCSS, CDFS and BDFS. With regard to high- and very high-risk patients, GS, RBP, prostate cancer staging and RT dose were predictors of PCSS, CDFS and BDFS in univariate analysis (UVA). CONCLUSION We found strong evidence that rectal and bladder preparation significantly decreases biochemical and clinical failures and the probability of death from PCa in patients treated without daily image-guided prostate localization, presumably since patients with RBP are able to maintain a reproducibly empty rectum and comfortably full bladder across the whole treatment compared with NRPB patients.", "gemini": "MỤC ĐÍCH VÀ MỤC TIÊU Kiểm tra giả thuyết rằng phác đồ chuẩn bị trực tràng và bàng quang có liên quan đến sự gia tăng tỷ lệ sống sót đặc hiệu ung thư tuyến tiền liệt (PCSS), tỷ lệ sống không có bệnh lâm sàng (CDFS) và tỷ lệ sống không có bệnh sinh hóa (BDFS). BỆNH NHÂN VÀ PHƯƠNG PHÁP Từ năm 1999 đến năm 2012, 1080 bệnh nhân ung thư tuyến tiền liệt (PCa) đã được điều trị bằng xạ trị theo khuôn ba chiều (3DCRT). Trong số những bệnh nhân này, 761 bệnh nhân được điều trị bằng phác đồ chuẩn bị trực tràng rỗng và bàng quang đầy thoải mái (RBP), trong khi 319 bệnh nhân không áp dụng phác đồ chuẩn bị trực tràng/bàng quang (NRBP). KẾT QUẢ So với bệnh nhân NRBP, bệnh nhân RBP có BDFS cao hơn đáng kể (lần lượt là 64% so với 48% sau 10 năm), CDFS (lần lượt là 81% so với 70,5% sau 10 năm) và PCSS (lần lượt là 95% so với 88% sau 10 năm) (kiểm tra log-rank p <0,001). Phân tích đa biến (MVA) chỉ ra đối với tất cả các bệnh nhân được điều trị và bệnh nhân có nguy cơ trung bình cao, điểm Gleason (GS) và chuẩn bị trực tràng và bàng quang là các yếu tố tiên lượng quan trọng nhất đối với PCSS, CDFS và BDFS. Đối với bệnh nhân có nguy cơ cao và rất cao, GS, RBP, giai đoạn ung thư tuyến tiền liệt và liều RT là các yếu tố dự báo PCSS, CDFS và BDFS trong phân tích đơn biến (UVA). KẾT LUẬN Chúng tôi tìm thấy bằng chứng mạnh mẽ cho thấy chuẩn bị trực tràng và bàng quang làm giảm đáng kể các thất bại về mặt sinh hóa và lâm sàng cũng như khả năng tử vong do PCa ở những bệnh nhân được điều trị mà không có định vị tuyến tiền liệt được hướng dẫn bằng hình ảnh hàng ngày, có lẽ là do bệnh nhân RBP có thể duy trì trực tràng rỗng có thể tái tạo và bàng quang đầy thoải mái trong toàn bộ quá trình điều trị so với bệnh nhân NRPB."} +{"idx": 2000138, "en": "OBJECTIVE This study aimed to investigate the advantages of recently developed cardiac imaging techniques of fat-water separation and feature tracking to characterize better individuals with chronic myocardial infarction (MI). MATERIALS AND METHODS Twenty patients who had a previous MI underwent CMR imaging. The study protocol included routine cine and late gadolinium enhancement (LGE) technique. In addition, mDixon LGE imaging was performed in every patient. Left ventricular (LV) circumferential (EccLV) and radial (ErrLV) strain were calculated using dedicated software (CMR42, Circle, Calgary, Canada). The extent of global scar was measured in LGE and fat-water separated images to compare conventional and recent CMR imaging techniques. RESULTS The infarct size derived from conventional LGE and fat-water separated images was similar. However, detection of lipomatous metaplasia was only possible with mDixon imaging. Subjects with fat deposition demonstrated a significantly smaller percentage of fibrosis than those without fat (10.68 ± 5.07% vs. 13.83 ± 6.30%; p = 0.005). There was no significant difference in EccLV or ErrLV between myocardial segments containing fibrosis only and fibrosis with fat. However, EccLV and ErrLV values were significantly higher in myocardial segments adjacent to fibrosis with fat deposition than in those adjacent to LGE only. CONCLUSIONS Advanced CMR imaging ensures more detailed tissue characterization in patients with chronic MI without a relevant increase in imaging and post-processing time. Fatty metaplasia may influence regional myocardial deformation especially in the myocardial segments adjacent to scar tissue. A simplified and shortened myocardial viability CMR protocol might be useful to better characterize and stratify patients with chronic MI.", "gemini": "MỤC TIÊU Nghiên cứu này nhằm mục đích tìm hiểu những ưu điểm của các kỹ thuật chụp ảnh tim mới phát triển là tách nước-mỡ và theo dõi tính năng để mô tả tốt hơn những cá nhân bị nhồi máu cơ tim mạn tính (MI). VẬT LIỆU VÀ PHƯƠNG PHÁP Hai mươi bệnh nhân đã từng bị nhồi máu cơ tim đã được chụp cộng hưởng từ (CMR). Giao thức nghiên cứu bao gồm kỹ thuật chụp động mạch vành thường quy và tăng cường gadolinium muộn (LGE). Ngoài ra, chụp LGE mDixon đã được thực hiện ở mọi bệnh nhân. Độ căng theo chu vi thất trái (EccLV) và hướng tâm (ErrLV) được tính toán bằng phần mềm chuyên dụng (CMR42, Circle, Calgary, Canada). Mức độ tổn thương toàn cầu được đo bằng hình ảnh LGE và hình ảnh tách nước-mỡ để so sánh các kỹ thuật chụp CMR thông thường và mới. KẾT QUẢ Kích thước nhồi máu thu được từ hình ảnh LGE thông thường và hình ảnh tách nước-mỡ là tương tự nhau. Tuy nhiên, chỉ có thể phát hiện ra loạn sản mỡ bằng cách chụp hình mDixon. Những đối tượng có sự lắng đọng mỡ cho thấy tỷ lệ xơ hóa nhỏ hơn đáng kể so với những đối tượng không có mỡ (10,68 ± 5,07% so với 13,83 ± 6,30%; p = 0,005). Không có sự khác biệt đáng kể về EccLV hoặc ErrLV giữa các phân đoạn cơ tim chỉ chứa xơ hóa và xơ hóa có mỡ. Tuy nhiên, giá trị EccLV và ErrLV cao hơn đáng kể ở các phân đoạn cơ tim tiếp giáp với xơ hóa có lắng đọng mỡ so với các phân đoạn chỉ tiếp giáp với LGE. KẾT LUẬN Chụp CMR tiên tiến đảm bảo mô tả mô chi tiết hơn ở những bệnh nhân bị nhồi máu cơ tim mạn tính mà không làm tăng đáng kể thời gian chụp và xử lý hậu kỳ. Loạn sản mỡ có thể ảnh hưởng đến sự biến dạng cơ tim khu vực, đặc biệt là ở các phân đoạn cơ tim tiếp giáp với mô sẹo. Một giao thức CMR đánh giá khả năng sống của cơ tim được đơn giản hóa và rút ngắn có thể hữu ích để mô tả và phân tầng tốt hơn những bệnh nhân bị nhồi máu cơ tim mạn tính."} +{"idx": 2000139, "en": "PURPOSE Cyclosporine A (CyA), a potent immunosuppressive agent used in renal transplantation, has a narrow therapeutic window and a large variability in blood concentrations. This study aimed to develop a population pharmacokinetic (PPK) model of CyA in living-donor renal transplant patients at a single center and identify factors influencing CyA pharmacokinetics (PK). METHODS A total of 660 points (preoperative) and 4785 points (postoperative) of blood concentration data from 98 patients who underwent renal transplantation were used. Pre- and postoperative CyA model structure and PPK parameters were separately estimated with a non-linear mixed-effect model, and subsequently, covariate analysis of postoperative data were comprehensively estimated, including preoperative PK parameters. RESULTS A two-compartment model with first-order absorption and absorption lag time was selected in this study. Aspartate aminotransferase, body surface area (BSA), pretransplant area under the whole blood concentration-time curve/dose, and postoperative days were identified as the covariates on oral clearance. BSA was selected as a covariate of the distribution volume of the central compartment. In addition, diabetes mellitus was selected as a covariate of the first-order absorption rate. CONCLUSIONS This PPK study used the largest number of blood concentration data among previous reports of living-donor renal transplant patients. Moreover, all patients received the same immunosuppressive regimen in a single center. Therefore, the validity of the selected covariates is reliable with high precision. The developed PPK model and selected covariates provide useful information about factors influencing CyA PK and greatly contributes to the identification of the most suitable dosing regimen for CyA.", "gemini": "MỤC ĐÍCH Cyclosporine A (CyA), một tác nhân ức chế miễn dịch mạnh được sử dụng trong ghép thận, có cửa sổ điều trị hẹp và nồng độ trong máu thay đổi lớn. Nghiên cứu này nhằm mục đích xây dựng mô hình dược động học quần thể (PPK) của CyA ở những bệnh nhân ghép thận từ người cho sống tại một trung tâm duy nhất và xác định các yếu tố ảnh hưởng đến dược động học (PK) của CyA. PHƯƠNG PHÁP Tổng cộng có 660 điểm (trước phẫu thuật) và 4785 điểm (sau phẫu thuật) dữ liệu nồng độ máu từ 98 bệnh nhân đã ghép thận được sử dụng. Cấu trúc mô hình CyA trước và sau phẫu thuật và các thông số PPK được ước tính riêng biệt bằng mô hình hiệu ứng hỗn hợp phi tuyến tính, và sau đó, phân tích hiệp biến của dữ liệu sau phẫu thuật được ước tính toàn diện, bao gồm các thông số PK trước phẫu thuật. KẾT QUẢ Một mô hình hai ngăn với sự hấp thụ bậc nhất và thời gian trễ hấp thụ đã được chọn trong nghiên cứu này. Aspartate aminotransferase, diện tích bề mặt cơ thể (BSA), diện tích dưới đường cong nồng độ toàn phần trong máu/liều trước khi ghép và ngày sau phẫu thuật được xác định là các hiệp biến về độ thanh thải qua đường uống. BSA được chọn làm hiệp biến của thể tích phân bố của ngăn trung tâm. Ngoài ra, đái tháo đường được chọn làm hiệp biến của tốc độ hấp thụ bậc nhất. KẾT LUẬN Nghiên cứu PPK này đã sử dụng số lượng dữ liệu nồng độ máu lớn nhất trong số các báo cáo trước đây về những bệnh nhân ghép thận từ người cho sống. Hơn nữa, tất cả bệnh nhân đều được áp dụng cùng một chế độ ức chế miễn dịch tại một trung tâm duy nhất. Do đó, tính hợp lệ của các hiệp biến được chọn là đáng tin cậy với độ chính xác cao. Mô hình PPK đã phát triển và các hiệp biến được chọn cung cấp thông tin hữu ích về các yếu tố ảnh hưởng đến PK của CyA và đóng góp rất lớn vào việc xác định chế độ dùng thuốc phù hợp nhất cho CyA."} +{"idx": 2000140, "en": "Although catheter ablation is a standard treatment for pediatric arrhythmias, there are no consensus guidelines for follow-up care. This study describes the variation in post-ablation practices identified through a survey of the pediatric and congenital electrophysiology society (PACES). Pediatric and congenital electrophysiology society members were invited to participate in an online survey of post-ablation practices in September 2014. Survey questions targeted routine post-ablation practices for three common arrhythmia substrates: atrioventricular nodal reentry tachycardia, concealed accessory pathways (AP), and manifest APs. Significant practice variation was defined as <90% concordance among respondents. There were 70 respondents from 67 centers, 29 (41%) in practice for <10 years. Uniform practices included aspirin after left side ablation by 65 (93%), immediate post-procedure ECG by 63 (90%), and performance of outpatient follow-up in 69 (99%) including ECG in 97-100% depending on substrate. The majority, 57 (81%), have standardized follow-up independent of substrate. Post-procedural observation is highly variable, with 25 (36%) discharging patients on the day of ablation, 22 (33%) observing patients in hospital overnight, and 21 (30%) basing hospitalization on pre-defined criteria. Immediate post-procedure echo is performed after all ablations in only 16 (23%). Discharge from outpatient care occurs at a median time of 12 months for each arrhythmia substrate. Common post-ablation practices are evident among pediatric electrophysiologists. However, they report significant variation in post-procedure monitoring practices and testing. The rationale for these variances, and their impact on costs and outcomes, should be defined.", "gemini": "Mặc dù cắt đốt bằng ống thông là phương pháp điều trị tiêu chuẩn cho chứng loạn nhịp tim ở trẻ em, nhưng không có hướng dẫn nào thống nhất về việc chăm sóc theo dõi. Nghiên cứu này mô tả sự khác biệt trong các phương pháp thực hành sau cắt đốt được xác định thông qua một cuộc khảo sát của Hiệp hội điện sinh lý nhi khoa và bẩm sinh (PACES). Các thành viên của Hiệp hội điện sinh lý nhi khoa và bẩm sinh được mời tham gia một cuộc khảo sát trực tuyến về các phương pháp thực hành sau cắt đốt vào tháng 9 năm 2014. Các câu hỏi khảo sát nhắm vào các phương pháp thực hành sau cắt đốt thường quy cho ba cơ chất loạn nhịp phổ biến: nhịp tim tái nhập nút nhĩ thất, đường dẫn phụ ẩn (AP) và AP biểu hiện. Sự khác biệt đáng kể trong thực hành được định nghĩa là sự nhất quán <90% giữa những người trả lời. Có 70 người trả lời từ 67 trung tâm, 29 (41%) hành nghề <10 năm. Các phương pháp thực hành thống nhất bao gồm aspirin sau khi cắt đốt bên trái bởi 65 (93%), điện tâm đồ ngay sau thủ thuật bởi 63 (90%) và thực hiện theo dõi ngoại trú ở 69 (99%) bao gồm điện tâm đồ ở mức 97-100% tùy thuộc vào cơ chất. Phần lớn, 57 (81%), có tiêu chuẩn theo dõi độc lập với cơ chất. Quan sát sau thủ thuật rất khác nhau, với 25 (36%) bệnh nhân xuất viện vào ngày cắt đốt, 22 (33%) theo dõi bệnh nhân tại bệnh viện qua đêm và 21 (30%) dựa vào các tiêu chí định sẵn để nhập viện. Siêu âm ngay sau thủ thuật chỉ được thực hiện sau khi cắt đốt ở 16 (23%). Xuất viện khỏi dịch vụ chăm sóc ngoại trú diễn ra ở thời điểm trung bình là 12 tháng đối với mỗi cơ chất loạn nhịp. Các phương pháp thực hành sau cắt đốt phổ biến là hiển nhiên ở các bác sĩ điện sinh lý nhi khoa. Tuy nhiên, họ báo cáo sự khác biệt đáng kể trong các phương pháp thực hành và xét nghiệm theo dõi sau thủ thuật. Cần xác định lý do cho những sai lệch này và tác động của chúng đối với chi phí và kết quả."} +{"idx": 2000141, "en": "PURPOSE Recurrence is a major clinical problem in patients with pulmonary embolism and can affect mortality. The decision to discontinue treatment is important for recurrence and is based on patients' clinical features as well as certain blood parameters. Our aim in this study was to evaluate whether mean platelet volume (MPV) and platelet distribution width (PDW) have utility as new predictive parameters for recurrence and mortality in pulmonary embolism. MATERIALS AND METHODS A total of 440 patients with pulmonary embolism underwent computed tomography, Doppler ultrasonography, and echocardiography before and at the conclusion of treatment. Thrombocyte count, MPV, PDW, and D-dimer parameters were also evaluated at the same time points. RESULTS MPV and PDW were significantly higher in deceased patients (8.8 ± 1.2 fl and 17.4 ± 0.8) compared to surviving patients (7.7 ± 0.9 fl and 17 ± 0.9) (p < 0.0001). Initial MPV and PDW were also significantly higher in patients with recurrence (8.4 ± 0.7 vs 7.6 ± 0.8 fl, p < 0.0001 and 17.3 ± 0.8 vs 16.9 ± 0.9, p = 0.002, respectively) than in patients without recurrence. At the end of treatment, MPV was still higher in patients with recurrence compared to patients without recurrence (8.7 ± 0.5 and 7.5 ± 0.7 fl, respectively, p < 0.0001). MPV values over 8.05 fl at the end of treatment predicted recurrence with 91% sensitivity and 77% specificity. CONCLUSION MPV seems to be an indicator of recurrence in pulmonary embolism and may have utility in the prediction of recurrence. Elevated MPV can also be used to predict mortality in pulmonary embolism.", "gemini": "MỤC ĐÍCH Tái phát là một vấn đề lâm sàng chính ở những bệnh nhân thuyên tắc phổi và có thể ảnh hưởng đến tỷ lệ tử vong. Quyết định ngừng điều trị rất quan trọng đối với tình trạng tái phát và dựa trên các đặc điểm lâm sàng của bệnh nhân cũng như một số thông số về máu nhất định. Mục đích của chúng tôi trong nghiên cứu này là đánh giá xem thể tích tiểu cầu trung bình (MPV) và độ rộng phân bố tiểu cầu (PDW) có hữu ích như những thông số dự đoán mới cho tình trạng tái phát và tử vong ở bệnh thuyên tắc phổi hay không. VẬT LIỆU VÀ PHƯƠNG PHÁP Tổng cộng 440 bệnh nhân thuyên tắc phổi đã được chụp cắt lớp vi tính, siêu âm Doppler và siêu âm tim trước và sau khi kết thúc điều trị. Các thông số đếm tiểu cầu, MPV, PDW và D-dimer cũng được đánh giá tại cùng thời điểm. KẾT QUẢ MPV và PDW cao hơn đáng kể ở những bệnh nhân tử vong (8,8 ± 1,2 fl và 17,4 ± 0,8) so với những bệnh nhân sống sót (7,7 ± 0,9 fl và 17 ± 0,9) (p < 0,0001). MPV và PDW ban đầu cũng cao hơn đáng kể ở những bệnh nhân tái phát (8,4 ± 0,7 so với 7,6 ± 0,8 fl, p < 0,0001 và 17,3 ± 0,8 so với 16,9 ± 0,9, p = 0,002) so với những bệnh nhân không tái phát. Vào cuối quá trình điều trị, MPV vẫn cao hơn ở những bệnh nhân tái phát so với những bệnh nhân không tái phát (lần lượt là 8,7 ± 0,5 và 7,5 ± 0,7 fl, p < 0,0001). Các giá trị MPV trên 8,05 fl vào cuối quá trình điều trị dự đoán tình trạng tái phát với độ nhạy 91% và độ đặc hiệu 77%. KẾT LUẬN MPV dường như là một chỉ báo về tình trạng tái phát ở bệnh thuyên tắc phổi và có thể có ích trong việc dự đoán tình trạng tái phát. MPV tăng cao cũng có thể được sử dụng để dự đoán tử vong ở bệnh thuyên tắc phổi."} +{"idx": 2000142, "en": "Cystic fibrosis (CF) is the most frequent lethal genetic disorder among Caucasians, but is considered to be a very rare disease in Chinese population. Here, we present an 11-year-old Chinese CF patient with disseminated bronchiectasis and salty sweat, for whom exon sequencing followed by multiplex ligation-dependent probe amplification analysis of the CFTR gene was applied for mutation screening. A homozygous deletion involving exon 20 of CFTR was observed in the patient's genome. Molecular characterization of the breakpoints indicated that both alleles of this locus had an identical novel complex rearrangement (c.3140-454_c.3367+249del931ins13, p.R1048_G1123del), leading to an in-frame removal of 76 amino acid residues in the second transmembrane domains of the CFTR protein. Although a same haplotype containing this complex rearrangement was observed on both of the maternal and paternal alleles, the parents denied any blood relationship as far as they know. Genome-wide homozygosity mapping was performed through SNP microarray and only a single homozygous interval of ~14.1 Mb at chromosome 7 containing the CFTR gene was observed, indicating the possible origin of the deletion from a common ancestor many generations ago. This study expands the mutation spectrum of CFTR in patients of Chinese origin and further emphasizes the necessity of MLPA analysis in mutation screening for CF patients.", "gemini": "Xơ nang (CF) là rối loạn di truyền gây tử vong phổ biến nhất ở người da trắng, nhưng được coi là một căn bệnh rất hiếm gặp ở người Trung Quốc. Ở đây, chúng tôi trình bày một bệnh nhân CF người Trung Quốc 11 tuổi bị giãn phế quản lan tỏa và mồ hôi mặn, người đã áp dụng giải trình tự exon tiếp theo là phân tích khuếch đại thăm dò phụ thuộc vào liên kết đa điểm của gen CFTR để sàng lọc đột biến. Một đột biến mất đoạn đồng hợp liên quan đến exon 20 của CFTR đã được quan sát thấy trong bộ gen của bệnh nhân. Đặc điểm phân tử của điểm gãy chỉ ra rằng cả hai alen của locus này đều có sự sắp xếp lại phức tạp mới giống hệt nhau (c.3140-454_c.3367+249del931ins13, p.R1048_G1123del), dẫn đến việc loại bỏ 76 phần tử axit amin trong khung ở miền xuyên màng thứ hai của protein CFTR. Mặc dù cùng một kiểu gen chứa sự sắp xếp lại phức tạp này đã được quan sát thấy trên cả hai alen của mẹ và cha, nhưng cha mẹ phủ nhận bất kỳ mối quan hệ huyết thống nào theo như họ biết. Bản đồ đồng hợp toàn bộ hệ gen đã được thực hiện thông qua mảng vi mô SNP và chỉ quan sát thấy một khoảng đồng hợp duy nhất khoảng 14,1 Mb ở nhiễm sắc thể 7 chứa gen CFTR, chỉ ra nguồn gốc có thể của đột biến xóa từ một tổ tiên chung nhiều thế hệ trước. Nghiên cứu này mở rộng phổ đột biến của CFTR ở những bệnh nhân gốc Hoa và nhấn mạnh thêm về sự cần thiết của phân tích MLPA trong sàng lọc đột biến cho bệnh nhân CF."} +{"idx": 2000143, "en": "Land use and land cover patterns are shaped by the interplay of human and ecological processes. Thus, heterogeneous cultural landscapes have developed, delivering multiple ecosystem services. To guarantee human well-being, the development of land use types has to be evaluated. Scenario development and land use and land cover change models are well-known tools for assessing future landscape changes. However, as social and ecological systems are inextricably linked, land use-related management decisions are difficult to identify. The concept of social-ecological resilience can thereby provide a framework for understanding complex interlinkages on multiple scales and from different disciplines. In our study site (Stubai Valley, Tyrol/Austria), we applied a sequence of steps including the characterization of the social-ecological system and identification of key drivers that influence farmers' management decisions. We then developed three scenarios, i.e., \"trend\", \"positive\" and \"negative\" future development of farming conditions and assessed respective future land use changes. Results indicate that within the \"trend\" and \"positive\" scenarios pluri-activity (various sources of income) prevents considerable changes in land use and land cover and promotes the resilience of farming systems. Contrarily, reductions in subsidies and changes in consumer behavior are the most important key drivers in the negative scenario and lead to distinct abandonment of grassland, predominantly in the sub-alpine zone of our study site. Our conceptual approach, i.e., the combination of social and ecological methods and the integration of local stakeholders' knowledge into spatial scenario analysis, resulted in highly detailed and spatially explicit results that can provide a basis for further community development recommendations.", "gemini": "Các mô hình sử dụng đất và che phủ đất được hình thành bởi sự tương tác giữa các quá trình của con người và sinh thái. Do đó, các cảnh quan văn hóa không đồng nhất đã phát triển, mang lại nhiều dịch vụ hệ sinh thái. Để đảm bảo phúc lợi của con người, cần phải đánh giá sự phát triển của các loại hình sử dụng đất. Phát triển kịch bản và các mô hình thay đổi sử dụng đất và che phủ đất là những công cụ nổi tiếng để đánh giá những thay đổi cảnh quan trong tương lai. Tuy nhiên, vì các hệ thống xã hội và sinh thái có mối liên hệ chặt chẽ với nhau nên rất khó để xác định các quyết định quản lý liên quan đến sử dụng đất. Do đó, khái niệm về khả năng phục hồi xã hội-sinh thái có thể cung cấp một khuôn khổ để hiểu các mối liên kết phức tạp trên nhiều quy mô và từ các ngành khác nhau. Tại địa điểm nghiên cứu của chúng tôi (Thung lũng Stubai, Tyrol/Áo), chúng tôi đã áp dụng một chuỗi các bước bao gồm mô tả hệ thống xã hội-sinh thái và xác định các động lực chính ảnh hưởng đến các quyết định quản lý của nông dân. Sau đó, chúng tôi đã phát triển ba kịch bản, tức là \"xu hướng\", \"tích cực\" và \"tiêu cực\" về sự phát triển trong tương lai của các điều kiện canh tác và đánh giá các thay đổi sử dụng đất tương ứng trong tương lai. Kết quả cho thấy rằng trong các kịch bản \"xu hướng\" và \"tích cực\", hoạt động đa dạng (nhiều nguồn thu nhập) ngăn ngừa những thay đổi đáng kể trong việc sử dụng đất và che phủ đất và thúc đẩy khả năng phục hồi của các hệ thống canh tác. Ngược lại, việc cắt giảm trợ cấp và thay đổi hành vi của người tiêu dùng là những động lực chính quan trọng nhất trong kịch bản tiêu cực và dẫn đến việc từ bỏ đồng cỏ rõ rệt, chủ yếu ở vùng cận núi tại địa điểm nghiên cứu của chúng tôi. Tiếp cận theo khái niệm của chúng tôi, tức là sự kết hợp giữa các phương pháp xã hội và sinh thái và việc tích hợp kiến thức của các bên liên quan tại địa phương vào phân tích kịch bản không gian, đã mang lại kết quả rất chi tiết và rõ ràng về mặt không gian, có thể cung cấp cơ sở cho các khuyến nghị phát triển cộng đồng trong tương lai."} diff --git a/retranslated_vi_pubmed/chunk_200/error.json b/retranslated_vi_pubmed/chunk_200/error.json new file mode 100644 index 0000000000000000000000000000000000000000..0637a088a01e8ddab3bf3fa98dbe804cbde1a0dc --- /dev/null +++ b/retranslated_vi_pubmed/chunk_200/error.json @@ -0,0 +1 @@ +[] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_201/completed.json b/retranslated_vi_pubmed/chunk_201/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..904354c6b345ad0325963927c75b9c4ea6122d3a --- /dev/null +++ b/retranslated_vi_pubmed/chunk_201/completed.json @@ -0,0 +1,9990 @@ +[ + 2010000, + 2010001, + 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0000000000000000000000000000000000000000..cd24c0a251faa3eba736011178129f2e872c2b82 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_212/data.jsonl @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:57730c93f334360208430c56c9e56ab22ea8fb0b1f75f67565085707ec78d310 +size 29821436 diff --git a/retranslated_vi_pubmed/chunk_212/error.json b/retranslated_vi_pubmed/chunk_212/error.json new file mode 100644 index 0000000000000000000000000000000000000000..b17c6417bfd3ebec2f32b0897b166dc3fc7b3618 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_212/error.json @@ -0,0 +1,8 @@ +[ + 2124204, + 2124684, + 2126946, + 2126947, + 2128304, + 2128443 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_213/completed.json b/retranslated_vi_pubmed/chunk_213/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..dcae13ae33c657d5d1b575b81ea848ffe4aa6907 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_213/completed.json @@ -0,0 +1,9996 @@ 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0000000000000000000000000000000000000000..96ea4120484a707b611b4be158ee31cef8a28df3 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_213/data.jsonl @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:8ab3bdb1b50fa1ce094a8d757a24a6e129ca01d8b7c8a19ba96aac936c74dc84 +size 28821262 diff --git a/retranslated_vi_pubmed/chunk_213/error.json b/retranslated_vi_pubmed/chunk_213/error.json new file mode 100644 index 0000000000000000000000000000000000000000..059d8c3348cd044a99b72088e8d5c634d939abc1 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_213/error.json @@ -0,0 +1,8 @@ +[ + 2135995, + 2138101, + 2139508, + 2139509, + 2139623, + 2139758 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_214/completed.json b/retranslated_vi_pubmed/chunk_214/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..a0639a610915c04277df69ba1ef1843da3d26949 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_214/completed.json @@ -0,0 +1,9990 @@ 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a/retranslated_vi_pubmed/chunk_215/data.jsonl b/retranslated_vi_pubmed/chunk_215/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..39e481d17d12256de7f22e4229e77ffa2662e372 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_215/data.jsonl @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:efe593c5324a16caad13c33d2d7f2d7db6558108cbea799ae04c00abc5b08b23 +size 30934320 diff --git a/retranslated_vi_pubmed/chunk_215/error.json b/retranslated_vi_pubmed/chunk_215/error.json new file mode 100644 index 0000000000000000000000000000000000000000..46a1ab600a2d4c1be043b3594527fb3c36244d75 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_215/error.json @@ -0,0 +1,5 @@ +[ + 2153347, + 2153737, + 2156375 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_216/completed.json b/retranslated_vi_pubmed/chunk_216/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..6e139c073de8bb6327311268096a9da1867f2c73 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a/retranslated_vi_pubmed/chunk_218/data.jsonl b/retranslated_vi_pubmed/chunk_218/data.jsonl new file mode 100644 index 0000000000000000000000000000000000000000..b07e01028bd8adf6c9158c81678566987e8042cd --- /dev/null +++ b/retranslated_vi_pubmed/chunk_218/data.jsonl @@ -0,0 +1,3 @@ +version https://git-lfs.github.com/spec/v1 +oid sha256:626c82f3b75e567deda5924110a18742c564ca1135b5b044b6e6e32e951a5e7e +size 31677711 diff --git a/retranslated_vi_pubmed/chunk_218/error.json b/retranslated_vi_pubmed/chunk_218/error.json new file mode 100644 index 0000000000000000000000000000000000000000..3a02076ff11f366b26a0c55ecb6e7b397d58dc66 --- /dev/null +++ b/retranslated_vi_pubmed/chunk_218/error.json @@ -0,0 +1,5 @@ +[ + 2182670, + 2184228, + 2187762 +] \ No newline at end of file diff --git a/retranslated_vi_pubmed/chunk_219/completed.json b/retranslated_vi_pubmed/chunk_219/completed.json new file mode 100644 index 0000000000000000000000000000000000000000..dec2588295501a1e910e88724a59b65ead9ad4fa 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