FUNCTION: Transcriptional repressor of genes encoding both fructose and glucose metabolic enzymes such as phosphofructokinase (PFK) or 2-keto- 3-deoxy-D-gluconate kinase (KDGK) during growth on glycerol. Required for the glycerol-mediated repression of pfkB-specific transcripts, but not needed for the high-levels of the pfkB-specific transcript present when cells are grown on fructose.


FUNCTION: Secreted XEG1-like protein that has lost enzyme activity but binds to host soybean apoplastic glucanase inhibitor protein GIP1 more tightly than does XEG1, thus it outcompetes XEG1 for GIP1 binding and frees functional XEG1 to support P.sojae infection (PubMed:28082413). With XEG1, is required to elevate apoplastic sugar during P.sojae infection (PubMed:28082413). SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the glycosyl hydrolase 12 (cellulase H) family.


FUNCTION: May be required for ribosome biogenesis. SUBCELLULAR LOCATION: Nucleus, nucleolus.


FUNCTION: Binds the chemical odorant, 2-isobutyl-3-methoxypyrazine. SIMILARITY: Belongs to the calycin superfamily. Lipocalin family.


SUBCELLULAR LOCATION: Cell inner membrane; Single-pass membrane protein. SIMILARITY: Belongs to the NapC/NirT/NrfH family.


FUNCTION: Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T- cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T- cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity (By similarity). SUBCELLULAR LOCATION: Early endosome membrane Recycling endosome membrane Late endosome Cytoplasmic vesicle, autophagosome Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Note=Localization to the endosome membrane is mediated via its interaction with WASHC2. SIMILARITY: Belongs to the WASH1 family.


FUNCTION: Vacuolar effluxer which mediate the efflux of amino acids resulting from autophagic degradation. The release of autophagic amino acids allows the maintenance of protein synthesis and viability during nitrogen starvation (By similarity). SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein Note=Vacuole and punctate structures. SIMILARITY: Belongs to the ATG22 family.


FUNCTION: Ferric reductase responsible for reducing extracellular iron and copper prior to import. Catalyzes the reductive uptake of Fe(3+)- salts and Fe(3+) bound to catecholate or hydroxamate siderophores. Fe(3+) is reduced to Fe(2+), which then dissociates from the siderophore and can be imported by the high-affinity Fe(2+) transport complex in the plasma membrane. Also participates in Cu(2+) reduction and Cu(+) uptake (By similarity). Involved in maintenance of cell wall integrity (CWI), mitochondrial function, and interaction between the pathogen and the host. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the ferric reductase (FRE) family.


SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the GRP transporter (TC 2.A.7.5) family.


FUNCTION: Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains. The RanBP2-type zinc finger (NZF) specifically recognizes Lys- 63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2: this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (By similarity). Involved in heart development (PubMed:20493459). SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein Cytoplasm, cytosol Note=Following IL1 stimulation, translocation occurs from the membrane to cytosol.


FUNCTION: Involved in the export and assembly of the SefA fimbrial subunit. SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the fimbrial export usher family.


FUNCTION: Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors. FUNCTION: Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3- phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense (By similarity). SUBCELLULAR LOCATION: Cytoplasm Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein Endosome membrane; Peripheral membrane protein Endoplasmic reticulum membrane; Peripheral membrane protein Mitochondrion membrane; Peripheral membrane protein Cytoplasmic vesicle, autophagosome Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. SUBCELLULAR LOCATION: [Beclin-1-C 37 kDa]: Mitochondrion. SUBCELLULAR LOCATION: [Beclin-1-C 35 kDa]: Mitochondrion Nucleus Cytoplasm. SIMILARITY: Belongs to the beclin family.


FUNCTION: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. SUBCELLULAR LOCATION: Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle. SIMILARITY: Belongs to the NPY family.


FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the diphthine synthase family.


FUNCTION: Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3- oxosteroids. It plays a central role in sexual differentiation and androgen physiology. SUBCELLULAR LOCATION: Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the steroid 5-alpha reductase family.


FUNCTION: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways. SIMILARITY: Belongs to the SsuE family.


FUNCTION: Binds directly to 26S ribosomal RNA. SIMILARITY: Belongs to the universal ribosomal protein uL11 family.


SIMILARITY: Belongs to the CCDC22 family.


FUNCTION: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Involved in cytokinesis. May play a role in HCV RNA replication. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). SUBCELLULAR LOCATION: Cytoplasm Cytoplasm, cytoskeleton, spindle Chromosome, centromere, kinetochore Cleavage furrow Midbody Cell projection, cilium, flagellum Note=In metaphase cells, localized within the microtubule spindle. At the metaphase plate, in close apposition to the kinetochores of the congressed chromosomes. In cells undergoing cytokinesis, localized to the midbody, the ingressing cleavage furrow, and the central spindle. Found in the sperm annulus (PubMed:25588830). SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.


FUNCTION: Transports S-adenosylmethionine. SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the drug/metabolite transporter (DMT) superfamily. 10 TMS drug/metabolite exporter (DME) (TC 2.A.7.3) family.


FUNCTION: Involved in sesquiterpene lactone biosynthesis. Produces exclusively (+)-germacrene A. SIMILARITY: Belongs to the terpene synthase family.


FUNCTION: RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. M6A demethylation by FTO affects mRNA expression and stability. Also able to demethylate m6A in U6 small nuclear RNA (snRNA). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA. Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping. Also acts as a tRNA demethylase by removing N(1)- methyladenine from various tRNAs. Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single- stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3- methylcytosine. Ability to repair alkylated DNA and RNA is however unsure in vivo. Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation. Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (By similarity). Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (By similarity). SUBCELLULAR LOCATION: Nucleus Nucleus speckle Cytoplasm Note=Localizes mainly in the nucleus, where it is able to demethylate N(6)-methyladenosine (m6A) and N(6),2'- O-dimethyladenosine cap (m6A(m)) in U6 small nuclear RNA (snRNA), N(1)- methyladenine from tRNAs and internal m6A in mRNAs. In the cytoplasm, mediates demethylation of m6A and m6A(m) in mRNAs and N(1)- methyladenine from tRNAs. SIMILARITY: Belongs to the fto family.


FUNCTION: Has a role in telomere length regulation and telomere end protection. Acts as an inhibitor of telomerase loading through its interaction with CDC13. SUBCELLULAR LOCATION: Nucleus Chromosome, telomere.


FUNCTION: Methylates the carboxyl group of several gibberellins (GAs). Substrate preference is GA9 > GA20 > GA3 > GA4 > GA34 > GA51 > GA1 > GA19 > GA12. No activity with diterpenes abietic acid and ent-kaurenoic acid. SIMILARITY: Belongs to the methyltransferase superfamily. Type-7 methyltransferase family. SABATH subfamily.


FUNCTION: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor (By similarity). SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the insulin family.


FUNCTION: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation (By similarity). SUBCELLULAR LOCATION: Nucleus Chromosome, centromere Note=Associates with centromeric constitutive heterochromatin. SIMILARITY: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.


FUNCTION: Transcriptional regulator. Regulates a number of genes involved in wing development including activation of net and bs and repression of rho and kni and controls vein-intervein patterning during wing development. Required for correct expression of a number of homeotic genes including Scr in the first leg imaginal disk and Ubx in the third leg imaginal disk and haltere disks. Required for stabilization of the histone-lysine N-methyltransferase trr and for trimethylation of 'Lys-4' of histone H3. Plays a role in maintenance of transcriptionally active chromatin through down-regulation of histone H1 hyperphosphorylation. SUBCELLULAR LOCATION: Nucleus Chromosome Note=Accumulates on salivary gland polytene chromosomes.


FUNCTION: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length. SIMILARITY: Belongs to the GreA/GreB family. GreB subfamily.


FUNCTION: May act as a substrate-specific adapter of an E3 ubiquitin- protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.


FUNCTION: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Hydrolyze 1-acyl-2-lyso-sn-glycero-3- phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3- phosphocholine (lyso-platelet-activating factor). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the neutral sphingomyelinase family.


FUNCTION: Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the BBP/SF1 family.


FUNCTION: Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT) activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin (CL), a key step in CL remodeling (PubMed:15152008). Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (PubMed:15152008). Also exhibits 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol- 3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn- glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Possesses lysophosphatidylinositol acyltransferase (LPIAT) activity (PubMed:20668164). Possesses lysophosphatidylglycerol acyltransferase (LPGAT) activity (By similarity). Required for establishment of the hematopoietic and endothelial lineages (PubMed:17675553). SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.


FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. SUBCELLULAR LOCATION: Late endosome membrane; Peripheral membrane protein Note=Recruited to the endosomal membrane in a VPS4A-dependent fashion. SIMILARITY: Belongs to the VPS37 family.


SUBCELLULAR LOCATION: Cytoplasm Nucleus. SIMILARITY: Belongs to the SAP18 family.


FUNCTION: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. SUBCELLULAR LOCATION: Mitochondrion inner membrane. SIMILARITY: Belongs to the cytochrome c oxidase subunit 5C family.


FUNCTION: Catalyzes the reduction of NADP(+) with F420H(2) via hydride transfer, and the reverse reaction, i.e. the reduction of F420 with NADPH. Probably functions in the regeneration of NADPH required in biosynthetic reactions. SIMILARITY: Belongs to the F420-dependent NADP reductase family.


FUNCTION: Transcription factor that is recruited by AHR1 to the promoters of genes involved in biofilm formation, which include several key adhesion genes. Plays an important role in cell adhesion, hyphal growth and virulence. Implicated in the regulation of opaque-phase- specific gene expression. SUBCELLULAR LOCATION: Nucleus.


FUNCTION: Bifunctional enzyme that catalyzes the decarboxylation of L- phenylalanine to produce 2-phenylethylamine, which is then oxidized to form 2-phenylacetaldehyde, a constituent of floral scent in petals (PubMed:16766535, PubMed:17928708). 2-phenylacetaldehyde is a precursor of 2-phenylethanol, another constituent of floral scent in petals (PubMed:16766535, PubMed:17928708). SIMILARITY: Belongs to the group II decarboxylase family.


SIMILARITY: Belongs to the XFP family.


FUNCTION: Transcriptional coactivator of the p300/CBP-mediated transcription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region. SUBCELLULAR LOCATION: Nucleus Note=Colocalizes with EP300 in dot-like structures. SIMILARITY: Belongs to the CITED family.


SIMILARITY: Belongs to the FAM167 (SEC) family.


FUNCTION: Has antimicrobial activity. SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the frog skin active peptide (FSAP) family. Dermaseptin subfamily.


FUNCTION: Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of OPG118 and OPG133, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase. SUBCELLULAR LOCATION: Virion Note=All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome. This is necessary because viral early mRNAs are synthesized within minutes after virus entry into the cell and are extruded through pores in the core particle. SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 19 kDa subunit family.


FUNCTION: Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SUBCELLULAR LOCATION: Lysosome.


FUNCTION: In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane porin. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity). SUBCELLULAR LOCATION: Periplasm.


FUNCTION: Catalyzes the dehydration of D-mannonate. Has no detectable activity with a panel of 70 other acid sugars (in vitro). SIMILARITY: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily.


FUNCTION: Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchor proteins (GPI-AP). Has phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI. Required for the shedding of the GPI-AP TDGF1, but not CFC1, at the cell surface. Shedding of TDGF1 modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Also indirectly involved in the translocation of RAC1 from the cytosol to the plasma membrane by maintaining the steady state amount of CAV1-enriched plasma membrane subdomains, stabilizing RAC1 at the plasma membrane (PubMed:27835684). In contrast to myomaker (TMEM8C), has no fusogenic activity (PubMed:26858401). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein Lysosome membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the TMEM8 family.


FUNCTION: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of SSC1 to substrate proteins and the association of SSC1 with TIM44. SUBCELLULAR LOCATION: Mitochondrion matrix. SIMILARITY: Belongs to the GrpE family.


FUNCTION: Exhibits both phospholipase A1/2 and acyltransferase activities (By similarity). Shows phospholipase A1 (PLA1) and A2 (PLA2) activity, catalyzing the calcium-independent release of fatty acids from the sn-1 or sn-2 position of glycerophospholipids (PubMed:21880860). Shows O-acyltransferase activity, catalyzing the transfer of a fatty acyl group from glycerophospholipid to the hydroxyl group of lysophospholipid (By similarity). Shows N-acyltransferase activity, catalyzing the calcium-independent transfer of a fatty acyl group at the sn-1 position of phosphatidylcholine (PC) and other glycerophospholipids to the primary amine of phosphatidylethanolamine (PE), forming N-acylphosphatidylethanolamine (NAPE), which serves as precursor for N-acylethanolamines (NAEs) (By similarity). SUBCELLULAR LOCATION: [Isoform 1]: Membrane; Single-pass membrane protein Cytoplasm. SUBCELLULAR LOCATION: [Isoform 2]: Nucleus Cytoplasm. SIMILARITY: Belongs to the H-rev107 family.


FUNCTION: May be involved in cell wall biosynthesis. May act as a fucosyltransferase. SUBCELLULAR LOCATION: Golgi apparatus. SIMILARITY: Belongs to the glycosyltransferase 37 family.


FUNCTION: Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: In the N-terminal section; belongs to the sulfate adenylyltransferase family. SIMILARITY: In the C-terminal section; belongs to the APS kinase family.


FUNCTION: Rho GTPase-activating protein involved in the signal transduction pathway (By similarity). Regulator of the contractile vacuole network as well as involved in driving vacuole emptying. SUBCELLULAR LOCATION: Cytoplasm Contractile vacuole Note=Localized to tubules of the contractile network.


FUNCTION: Probably important in the regulation of neuronal excitability. May underlie a potassium current involved in regulating the excitability of sensory cells of the cochlea. SUBCELLULAR LOCATION: Basal cell membrane; Multi-pass membrane protein. Note=Situated at the basal membrane of cochlear outer hair cells. SIMILARITY: Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.4/KCNQ4 sub-subfamily.


FUNCTION: Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development (By similarity). SIMILARITY: Belongs to the RCAN family.


FUNCTION: The VP3 protein is one of the five proteins (with VP1, VP4, VP6 and VP7) which form the inner capsid of the virus. SUBCELLULAR LOCATION: Virion. SIMILARITY: Belongs to the orbivirus VP3 family.


FUNCTION: Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import. SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the CbiN family.


SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.


FUNCTION: Displays bipolar ssDNA and dsDNA unwinding activities that require the same core catalytic residues for unwinding in either direction, the 3'-5' direction being more robust. SIMILARITY: Belongs to the herpesviridae oribp family.


FUNCTION: Negative regulator of sphingolipid synthesis. FUNCTION: Negative regulator of sphingolipid synthesis. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the ORM family.


FUNCTION: Catalyzes the oxidation of (S)-lactate (L-lactate) to pyruvate with subsequent transfer of electrons to cytochrome c (PubMed:11914072). Is involved in the utilization of (S)-lactate as a sole source of carbon for growth (PubMed:3004948). Can also use ferricyanide as an electron acceptor in vitro (PubMed:4593578, PubMed:3004948). SUBCELLULAR LOCATION: Mitochondrion intermembrane space. SIMILARITY: In the N-terminal section; belongs to the cytochrome b5 family. SIMILARITY: In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.


FUNCTION: Maintains high levels of reduced glutathione (GSH) in the cytosol by reducing glutathione disulfide (oxidized glutathione or GSSG) (PubMed:16678813). The amount of GSH may affect the determination of cell fate (PubMed:16678813). SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.


FUNCTION: Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde. SIMILARITY: Belongs to the PTPS family. QueD subfamily.


FUNCTION: Snake venom phospholipase A2 (PLA2) that significantly inhibits ADP-induced platelet aggregation in platelet-rich plasma of human, rabbit and guinea pig. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the phospholipase A2 family. Group II subfamily. D49 sub-subfamily.


FUNCTION: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. May have a role in DNA repair mechanism and support vegetative growth of the cells. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. SIMILARITY: Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.


FUNCTION: 2-oxoglutarate-dependent dioxygenase; part of the gene cluster that mediates the biosynthesis of pneumocandins, lipohexapeptides of the echinocandin family that prevent fungal cell wall formation by non-competitive inhibition of beta-1,3-glucan synthase (PubMed:27705900). The 10,12-dimethylmyristoyl side chain is synthesized by the reducing polyketide synthase gloL/GLPKS4 (PubMed:27494047). The thioesterase gloN/GLHYD exclusively interacts with gloL/GLPKS4 to maintain turnover of the polyketide side chain (PubMed:27494047). The 10R,12S-dimethylmyristic acid is then transferred to the first thiolation domain of the nonribosomal peptide synthetase gloA/GLNRPS4 by the acyl-AMP ligase gloD/GLligase, followed by its acylation to L-ornithine to trigger elongation of the cyclic hexapeptide (PubMed:27494047). L-ornithine, 4R-hydroxyl-L-proline (generated from L-proline by the dioxygenase gloF/GLOXY2), 3S-hydroxyl- L-homotyrosine (generated by gloG/GLHtyB, gloH/GLHtyA, gloI/GLHtyC, gloJ/GLHtyD and hydroxylated at C-3 by the dioxygenase gloM/GLOXY1), 3R-hydroxyl-L-glutamine (generated from L-glutamine probably by the dioxygenase gloE/GLOXY3) and 3S-hydroxyl-L-proline (generated from L- proline by the dioxygenase gloF/GLOXY2 to yield pneumocandin B0), or 3S-hydroxyl-4S-methyl-L-proline (generated from L-leucine by the dioxygenase gloC/GLOXY4 to yield pneumocandin A0) are sequentially added to the growing chain (PubMed:25270390, PubMed:25879325, PubMed:25527531). The last C domain of gloA/GLNRPS4 is proposed to be responsible for cyclization by condensation to form the peptide bond between L-ornithine and 3S-hydroxyl-4S-methyl-L-proline (for pneumocandin A0) or 3S-hydroxyl-L-proline (for pneumocandin B0). Finally, the subsequent C-4 hydroxylation of 3S-hydroxyl-L-homotyrosine and L-ornithine dihydroxylation at C-4 and C-5 are performed by the cytochrome P450 monooxygenases gloP/GLP450-1 and gloO/GLP450-2, respectively (PubMed:25879325). SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase family.


FUNCTION: A component of the complex of water-borne protein pheromones that stimulates attraction and mating behavior. SUBCELLULAR LOCATION: Secreted.


SIMILARITY: Belongs to the LOB domain-containing protein family.


FUNCTION: Plays a role in the bacterium-induced formation of multinucleated giant cell (MNGC), which is formed after host cell fusion, as well as in the intercellular spreading of bacteria and in the induction of apoptosis in macrophages. May act in concert with other effector proteins to induce fusion of host cell membranes (By similarity). SUBCELLULAR LOCATION: Secreted Host membrane Note=Secreted via the bsa type III secretion system, and probably inserted into host membranes. SIMILARITY: Belongs to the invasin protein B family.


FUNCTION: Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF- ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. SUBCELLULAR LOCATION: Mitochondrion matrix. SIMILARITY: Belongs to the ETF alpha-subunit/FixB family.


FUNCTION: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle. SUBCELLULAR LOCATION: Cytoplasm Nucleus, nucleolus. SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.


FUNCTION: Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway (By similarity). SUBCELLULAR LOCATION: Nucleus, nucleolus. SIMILARITY: Belongs to the RRP36 family.


FUNCTION: Uniporter that mediates the transport of the stereospecific L-phenylalanine, L-methionine and L-branched-chain amino acids, between the extracellular space and the cytoplasm and may control the transepithelial (re)absorption of neutral amino acid in kidney and small intestine. The transport activity is mediated through facilitated diffusion and is sodium ions-, chloride ions- and pH-independent. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein Basolateral cell membrane Note=Located at the basolateral membrane in the small intestine enterocytes, kidney proximal tubule, thick ascending limb and, to a minor extent, of distal convoluted tubule epithelial cells. SIMILARITY: Belongs to the SLC43A transporter (TC 2.A.1.44) family.


FUNCTION: Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells (PubMed:20154091, PubMed:20937701, PubMed:24520163, PubMed:26774822). Acts by promoting the exchange of GDP to GTP, converting inactive GDP- bound RAB35 into its active GTP-bound form (PubMed:20154091, PubMed:20937701). Plays a role in clathrin-mediated endocytosis (PubMed:20154091). Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity (PubMed:26774822). Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells (PubMed:26774822). SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasmic vesicle, clathrin-coated vesicle.


FUNCTION: F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the ATPase C chain family.


FUNCTION: Converts the aldose L-fucose into the corresponding ketose L- fuculose. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the L-fucose isomerase family.


SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein. SIMILARITY: Belongs to the cortexin family.


FUNCTION: Component of the spliceosome and rRNA processing machinery. In association with the spliceosomal U4/U6.U5 tri-snRNP particle, required for splicing of pre-mRNA (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the SPP381 family.


SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein Spore coat. Note=Associated with the inner face of spore coat. SIMILARITY: Belongs to the SCAMP family.


FUNCTION: Involved in the proteasome-dependent degradation of fructose- 1,6-bisphosphatase. SUBCELLULAR LOCATION: Cytoplasm Nucleus. SIMILARITY: Belongs to the FYV10 family.


FUNCTION: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'- 5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities. SIMILARITY: Belongs to the DNA repair enzymes AP/ExoA family.


FUNCTION: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium. SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side Cell projection Cytoplasm, cytoskeleton Nucleus Note=Colocalizes with MPP1 in non-myelin-forming Schwann cells. Binds with DCAF1 in the nucleus. The intramolecular association of the FERM domain with the C-terminal tail promotes nuclear accumulation. The unphosphorylated form accumulates predominantly in the nucleus while the phosphorylated form is largely confined to the non-nuclear fractions (By similarity).


FUNCTION: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the eukaryotic ribosomal protein eL30 family.


FUNCTION: Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr). The two antagonistic activities of HprK/P are regulated by several intracellular metabolites, which change their concentration in response to the absence or presence of rapidly metabolisable carbon sources (glucose, fructose, etc.) in the growth medium. Also phosphorylates/dephosphorylates the HPr-like catabolite repression protein crh on a specific serine residue. Therefore, by controlling the phosphorylation state of HPr and crh, HPrK/P is a sensor enzyme that plays a major role in the regulation of carbon metabolism and sugar transport: it mediates carbon catabolite repression (CCR), and regulates PTS-catalyzed carbohydrate uptake and inducer exclusion (By similarity). SIMILARITY: Belongs to the HPrK/P family.


FUNCTION: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the CybS family.


SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the DNAJC25 family.


FUNCTION: Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response (PubMed:9448728, PubMed:10438452). The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes (PubMed:10438452). This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (By similarity). Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3- series prostaglandins (By similarity). In an alternative pathway of prostanoid biosynthesis, converts 2-arachidonoyl lysophopholipids to prostanoid lysophopholipids, which are then hydrolyzed by intracellular phospholipases to release free prostanoids (By similarity). Metabolizes 2-arachidonoyl glycerol yielding the glyceryl ester of PGH2, a process that can contribute to pain response (By similarity). Plays a role in the generation of resolution phase interaction products (resolvins) during both sterile and infectious inflammation (By similarity). Metabolizes docosahexaenoate (DHA, C22:6(n-3)) to 17R-HDHA, a precursor of the D-series resolvins (RvDs) (By similarity). As a component of the biosynthetic pathway of E-series resolvins (RvEs), converts eicosapentaenoate (EPA, C20:5(n-3)) primarily to 18S-HEPE that is further metabolized by ALOX5 and LTA4H to generate 18S-RvE1 and 18S- RvE2 (By similarity). In vascular endothelial cells, converts docosapentaenoate (DPA, C22:5(n-3)) to 13R-HDPA, a precursor for 13- series resolvins (RvTs) shown to activate macrophage phagocytosis during bacterial infection (By similarity). In activated leukocytes, contributes to oxygenation of hydroxyeicosatetraenoates (HETE) to diHETES (5,15-diHETE and 5,11-diHETE) (By similarity). During neuroinflammation, plays a role in neuronal secretion of specialized preresolving mediators (SPMs) 15R-lipoxin A4 that regulates phagocytic microglia (By similarity). Can also use linoleate (LA, (9Z,12Z)- octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)- HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products (PubMed:9448728). SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein Endoplasmic reticulum membrane; Peripheral membrane protein Nucleus inner membrane; Peripheral membrane protein Nucleus outer membrane; Peripheral membrane protein Note=Detected on the lumenal side of the endoplasmic reticulum and nuclear envelope. SIMILARITY: Belongs to the prostaglandin G/H synthase family.


FUNCTION: Catalyzes the exchange of L-arginine for agmatine. The arginine uptake by the bacterium in the macrophage may be a virulence factor against the host innate immune response. SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2) family.


FUNCTION: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+) excretion. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit G family.


FUNCTION: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3- hydroxy-4-methylpentanoate (2-isopropylmalate). SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.


FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. SIMILARITY: Belongs to the Wnt family.


FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state (PubMed:17325221). Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the type II topoisomerase GyrB family.


FUNCTION: Nucleoside diphosphate sugar hydrolase that hydrolyzes GDP- mannose as its preferred substrate, yielding GMP and mannose-1- phosphate. SIMILARITY: Belongs to the Nudix hydrolase family. NudK subfamily.


FUNCTION: Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane. SUBCELLULAR LOCATION: Mitochondrion Note=Mitoribosomes are tethered to the mitochondrial inner membrane and spatially aligned with the membrane insertion machinery through two distinct membrane contact sites, formed by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein MBA1. SIMILARITY: Belongs to the bacterial ribosomal protein bS6 family.


FUNCTION: Catalyzes the conversion of taxusin to 2-alpha-hydroxytaxusin in taxol biosynthesis (PubMed:15178487). Catalyzes the conversion of 7- beta-hydroxytaxusin to 2-alpha-7-beta-hydroxytaxusin in taxol biosynthesis (PubMed:15178487). SUBCELLULAR LOCATION: Microsome membrane; Single-pass membrane protein. SIMILARITY: Belongs to the cytochrome P450 family.


FUNCTION: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (By similarity). Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides (By similarity). Also has phospholipase activity, and can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity) (By similarity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH (By similarity). Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis (By similarity). Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (By similarity). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate (By similarity). SUBCELLULAR LOCATION: Cytoplasm Lysosome Note=Also found in lung secretory organelles (lamellar bodies). SIMILARITY: Belongs to the peroxiredoxin family. Prx6 subfamily.


FUNCTION: Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the eukaryotic ribosomal protein eL15 family.


FUNCTION: Suppressor of bem1/bud5. SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein. SIMILARITY: Belongs to the serine esterase family.


FUNCTION: Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and LsrD permeases (By similarity). SUBCELLULAR LOCATION: Periplasm. SIMILARITY: Belongs to the bacterial solute-binding protein 2 family.


FUNCTION: Probable ion channel. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the TMC family.


FUNCTION: Catalyzes the ligation of benzoate and CoA to form benzoyl- CoA at the expense of ATP. The enzyme also ligates 2-aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivatives. SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily.


FUNCTION: Calcium selective cation channel that mediates Ca(2+) uptake in various tissues, including the intestine (PubMed:11097838, PubMed:11278579, PubMed:11248124, PubMed:15184369, PubMed:23612980, PubMed:29258289). Important for normal Ca(2+) ion homeostasis in the body, including bone and skin (By similarity). The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification (PubMed:15184369). Inactivation includes both a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism; the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV6 sub-subfamily.


FUNCTION: [Isoform 1]: Efficiently hydrolyzes cAMP. FUNCTION: Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes. FUNCTION: [Isoform 2]: Efficiently hydrolyzes cAMP. SUBCELLULAR LOCATION: [Isoform 2]: Cytoplasm, cytosol Membrane; Peripheral membrane protein. SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.


FUNCTION: Cysteine protease that plays an essential role in host liquefaction to facilitate horizontal transmission of the virus. May participate in the degradation of foreign protein expressed by the baculovirus system (By similarity). SIMILARITY: Belongs to the peptidase C1 family.


FUNCTION: Promotes both transcription and replication of genomic RNA. Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. May interact with host RNA polymerase II thereby changing its template requirement from DNA to RNA. RNA pol II complex would then acts as an RNA-directed RNA polymerase, and transcribe and replicate HDV genome (By similarity). SUBCELLULAR LOCATION: Virion. Host nucleus. SIMILARITY: Belongs to the hepatitis delta antigen family.


FUNCTION: Probable transcription factor, required for migration of the hermaphrodite-specific motor neurons (HSNs) from the tail to the gonad primordium during HSN cell differentiation (PubMed:8224840, PubMed:10049362). Required for phasmid neuron development (PubMed:8224840). Required to specify the pi-cell fate of ventral uterine precursor cell (VU) cells (PubMed:17573066). FUNCTION: [Isoform b]: Dispensable for anchor cell (AC) invasion and for preventing AC proliferation. FUNCTION: [Isoform a]: Probable transcription factor, involved in lin- 12 (Notch)-dependent anchor cell (AC) and ventral uterine (VU) precursor cell fate specification and in AC invasion (PubMed:17215301, PubMed:17573066, PubMed:32203506). Prevents AC proliferation after AC cell specification by repressing lin-12 expression (PubMed:32203506). May form a positive feedback loop, together with the transcription factor fos-1, that maintains mutual high levels of expression and so activates AC invasion (PubMed:32203506). SUBCELLULAR LOCATION: Nucleus.


FUNCTION: Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system.


FUNCTION: Acts as a positive regulator of apoptosis. May also act as a regulator of cellular reactive oxygen species. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.


FUNCTION: Catalyzes the dehydration of trans-3-hydroxy-L-proline to delta-1-pyrroline-2-carboxylate (Pyr2C). SIMILARITY: Belongs to the proline racemase family.


FUNCTION: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein; Extracellular side. SIMILARITY: Belongs to the peptidase M10A family.


FUNCTION: Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. Acts as an adapter that recruits the DOA4 deubiquitinase to the endosomes, leading to deubiquitination of cargo proteins prior to the lumenal sequestration. Its association to the endosomes depends on SNF7 and its dissociation requires VPS4. Interacts functionally with the Pkc1p- mitogen-activated protein kinase pathway. SUBCELLULAR LOCATION: Cytoplasm. Endosome.


SIMILARITY: In the N-terminal section; belongs to the ChdC family.


FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.


FUNCTION: Key enzyme for ketone body catabolism (By similarity). Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production (PubMed:11327867, PubMed:17718512) (Probable). Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl- CoA (PubMed:11327867). Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (PubMed:17718512). SUBCELLULAR LOCATION: Mitochondrion. SIMILARITY: Belongs to the 3-oxoacid CoA-transferase family.


FUNCTION: Converts fumarylacetoacetate to acetoacetate and fumarate (PubMed:22980205). Involved in tyrosine catabolic pathway. Catalyzes the final step in the tyrosine degradation pathway (PubMed:22980205, PubMed:23743712, PubMed:27097641). SIMILARITY: Belongs to the FAH family.


FUNCTION: Essential arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA). Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins SmD1 and SmD3 (By similarity). SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family. PRMT7 subfamily.


FUNCTION: May be involved in cadmium resistance. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the cornifelin family.


FUNCTION: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. SUBCELLULAR LOCATION: Cytoplasm Nucleus Cytoplasm, cytosol Note=Can form fiber-like subcellular structures termed 'cytoophidia' in response to intracellular guanine- nucleotide depletion. SIMILARITY: Belongs to the IMPDH/GMPR family.


FUNCTION: Binds lactose. May participate in the apoptosis of adipocytes. SUBCELLULAR LOCATION: Nucleus.


FUNCTION: [Double-strand-break repair protein rad21 homolog]: As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions (PubMed:11509732). The cohesin complex may also play a role in spindle pole assembly during mitosis (PubMed:11590136). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter (PubMed:25575569). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity). FUNCTION: [64-kDa C-terminal product]: May promote apoptosis. SUBCELLULAR LOCATION: [64-kDa C-terminal product]: Cytoplasm, cytosol Nucleus. SUBCELLULAR LOCATION: [Double-strand-break repair protein rad21 homolog]: Nucleus Nucleus matrix Chromosome Chromosome, centromere Cytoplasm, cytoskeleton, spindle pole Note=Associates with chromatin (PubMed:11590136, PubMed:11073952). Before prophase, scattered along chromosome arms (PubMed:11073952). During prophase and prometaphase, most cohesins dissociate from the arms of condensing chromosome, possibly through PLK1-mediated phosphorylation (PubMed:11931760). A small amount of cohesin remains in centromeric regions and is removed from chromosomes only at the onset of anaphase. At anaphase, cleavage by separase/ESPL1 leads to the dissociation of cohesin from chromosomes and chromosome separation (PubMed:11073952, PubMed:11509732). SIMILARITY: Belongs to the rad21 family.


FUNCTION: Catalyzes a trans-dehydration via an enolate intermediate. Is involved in both the catabolism of quinate and the biosynthesis of aromatic amino acids. SIMILARITY: Belongs to the type-II 3-dehydroquinase family.


FUNCTION: A metamorphic protein which reversibly switches between an inactive tetrameric fold and a rare, thioredoxin-like monomeric fold (KaiB(fs)). KaiB(fs) binds phospho-KaiC, KaiA and CikA. KaiA and CikA compete for binding to KaiB(fs), and KaiB(fs) and SasA compete for binding to KaiC, thus the clock oscillator and output signal pathway are tightly coupled. FUNCTION: Key component of the KaiABC oscillator complex, which constitutes the main circadian regulator in cyanobacteria. Complex composition changes during the circadian cycle to control KaiC phosphorylation. KaiA stimulates KaiC autophosphorylation, while KaiB sequesters KaiA, leading to KaiC autodephosphorylation. Phospho-Ser-431 KaiC accumulation triggers binding of KaiB to form the KaiB(6):KaiC(6) complex, leading to changes in output regulators CikA and SasA. KaiB switches to a thioredoxin-like fold (KaiB(fs)) when bound to KaiC. KaiB(6):KaiC(6) formation exposes a site for KaiA binding that sequesters KaiA from KaiC, making the KaiC(6):KaiB(6):KaiA(12) complex that results in KaiC autodephosphorylation. SIMILARITY: Belongs to the KaiB family.


FUNCTION: Activates expression of the rhaSR operon in response to L- rhamnose. SUBCELLULAR LOCATION: Cytoplasm.


FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.


FUNCTION: Responsible for synthesis of murine T-lymphocyte CT antigen. Can transfer N-acetylgalactosamine moiety from UDP-GalNAc to the low molecular weight acceptor 3'-sialyl-N-acetyllactosamine, to form a non- reducing terminal tetrasaccharide Sda blood group structure. SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein. SIMILARITY: Belongs to the glycosyltransferase 2 family.


FUNCTION: Activates acetoacetate to acetoacetyl-CoA. SUBCELLULAR LOCATION: Cytoplasm, cytosol. SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.


FUNCTION: Has bactericidal activity. SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the beta-defensin family.


FUNCTION: Plays a role in the regulation of cell shape and polarity (By similarity). Plays a role in cellular protein transport, including protein transport away from primary cilia (By similarity). Neuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus (By similarity). Competes with SIAH1 for binding GAPDH (By similarity). Does not regulate lysosomal morphology and distribution (PubMed:14668488). Binds to RAB10 following LRRK2-mediated RAB10 phosphorylation which leads to inhibition of ciliogenesis (PubMed:30398148). SUBCELLULAR LOCATION: Cytoplasm, cytosol Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasm, cytoskeleton, cilium basal body. SIMILARITY: Belongs to the RILPL family.


FUNCTION: Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (PubMed:34715025). May play an important role for the maintenance of myelin-axon integrity (By similarity). May affect intracellular Ca(2+) homeostasis (By similarity). SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme Cytoplasm Cytoplasm, cytoskeleton.


FUNCTION: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity). Activated by the signaling pathway regulator NMI which acts as damage- associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (By similarity). SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein Early endosome Cell projection, ruffle Note=Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis. Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation. SIMILARITY: Belongs to the Toll-like receptor family.


FUNCTION: Triggers stress-induced chloroplast degradation, independently of autophagy and senescence-associated vacuoles (PubMed:25538186). After targeting to the chloroplast, triggers its destabilization and subsequent disassembly, inducing the formation of CV-containing vesicles (CCVs) carrying stromal proteins, envelope membrane proteins, and thylakoid membrane proteins which are released from the chloroplasts and mobilized to the vacuole for proteolysis (PubMed:25538186). SUBCELLULAR LOCATION: Plastid, chloroplast membrane; Single-pass membrane protein Plastid, chloroplast thylakoid membrane; Single-pass membrane protein Plastid, chloroplast envelope Vacuole Vesicle Note=Present in vesicle-like spots observed in destabilized chloroplasts; these CV-containing vesicles (CCVs) carrying stromal proteins, envelope membrane proteins, and thylakoid membrane proteins translocate later to the cytosol before being mobilized to the vacuole for proteolysis.


FUNCTION: Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein Golgi apparatus membrane; Single-pass type I membrane protein Note=Recycles between endoplasmic reticulum and Golgi. SIMILARITY: Belongs to the EMP24/GP25L family.


FUNCTION: Carrier of the growing fatty acid chain in fatty acid biosynthesis (PubMed:1907568). Accessory and non-catalytic subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain (PubMed:1907568, PubMed:10852722, PubMed:18721790). Accessory protein, of the core iron-sulfur cluster (ISC) assembly complex, that regulates, in association with LYRM4, the stability and the cysteine desulfurase activity of NFS1 and participates in the [2Fe-2S] clusters assembly on the scaffolding protein ISCU (By similarity). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (By similarity). SUBCELLULAR LOCATION: Mitochondrion. SIMILARITY: Belongs to the acyl carrier protein (ACP) family.


FUNCTION: Corepressor targeting diverse transcription regulators. Has dehydrogenase activity. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.


FUNCTION: Proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (By similarity). SUBCELLULAR LOCATION: Mitochondrion membrane; Multi-pass membrane protein Mitochondrion inner membrane.


FUNCTION: Calcium, potassium:sodium antiporter that transports 1 Ca(2+) and 1 K(+) in exchange for 4 Na(+). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.


FUNCTION: Inclusion membrane protein probably involved in early modification events of the chlamydial inclusion. SUBCELLULAR LOCATION: Secreted Host vacuole, host pathogen-containing vacuole, host pathogen-containing vacuole membrane; Multi-pass membrane protein Note=Secreted, probably by a type III secretion system (Probable). Localized in the inclusion membrane (PubMed:10447885, PubMed:11207546, PubMed:26416906). Inclusion membrane staining is punctate (PubMed:10447885). The C-terminus faces the host cytosol (PubMed:11207546).


FUNCTION: Carrier protein involved in the D-alanylation of lipoteichoic acid (LTA). The loading of thioester-linked D-alanine onto DltC is catalyzed by D-alanine--D-alanyl carrier protein ligase DltA. The DltC- carried D-alanyl group is further transferred to cell membrane phosphatidylglycerol (PG) by forming an ester bond, probably catalyzed by DltD. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the DltC family.


FUNCTION: Blocker of A-type voltage-gated potassium channels of cerebellar granular cells. May also inhibit Kv4/KCND when coexpressed with DPP6 or DPP10. The occlusion of the outer entry of the K(+) conducting pore is partially reversible and affects both open and closed channels. It shares the same target in rat brain than BmTX3 (AC Q8I0L5) and AmmTX3 (AC P60208). Recombinant toxin inhibits mKv1.3/KCNA3 channel (IC(50)=0.49 nM) (PubMed:22580271). SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 15 subfamily.


FUNCTION: Responsible for synthesis of pseudouridine from uracil-2457 in 23S ribosomal RNA. SIMILARITY: Belongs to the pseudouridine synthase RsuA family.


SIMILARITY: Belongs to the MTFR1 family.


FUNCTION: Component of the set3 histone deacetylase complex which is involved in chromatin remodeling and transcription regulation, such as repression of the sporulation gene program. SUBCELLULAR LOCATION: Cytoplasm Nucleus.


SIMILARITY: To M.jannaschii MJ0977.


FUNCTION: E3 proteins seem to be dispensable for virus growth in tissue culture cells. They are potentially important for virus growth under special conditions; E3 region may help adenoviruses to evade the immune surveillance of the host. SIMILARITY: Belongs to the adenoviridae E3_20 family.


FUNCTION: The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH). SUBCELLULAR LOCATION: Mitochondrion. SIMILARITY: Belongs to the GcvP family.


FUNCTION: Stimulates insulin release from beta cells at a concentration of 1 nM and release of glucagon-like peptide 1 (GLP-1) from enteroendocrine cells in vitro. Reduces secretion of interferon gamma from peritoneal cells in a mouse model. Has no inhibitory activity against Gram-positive bacterium B.megaterium Bm11 or Gram-negative bacterium E.coli ATCC 25922 at concentrations of up to 100 uM. Has no hemolytic activity against mouse erythrocytes. SUBCELLULAR LOCATION: Secreted.


FUNCTION: Confers resistance to fosfomycin and deoxycholate. SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein. SIMILARITY: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily.


FUNCTION: Snake venom zinc metalloproteinase that cleaves both alpha- and beta-chains of fibrinogen, but not the gamma-chain (PubMed:29761254). SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the venom metalloproteinase (M12B) family. P-II subfamily. P-IIa sub-subfamily.


FUNCTION: Major beta-glucosidase activating oleuropein into a potent protein cross-linking agent (PubMed:25697790, PubMed:28483880). No activity with rutin, luteolin or p-nitrophenyl-beta-glucopyranoside as substrates (PubMed:25697790). SUBCELLULAR LOCATION: Nucleus Note=Distinctive compartmentalization of the oleuropein/OeGLU dual partner is a requisite to ensure a correct defense system response and to prevent the autotoxicity. SIMILARITY: Belongs to the glycosyl hydrolase 1 family.


FUNCTION: General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA) (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the transcriptional coactivator PC4 family.


FUNCTION: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)- enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity (PubMed:30057030). It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2 (PubMed:29290614). SUBCELLULAR LOCATION: Mitochondrion matrix. SIMILARITY: Belongs to the type IA topoisomerase family.


SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.


FUNCTION: Probable O-linked N-acetylglucosamine transferase (OGT) involved in various processes such as gibberellin (GA) signaling pathway. OGTs catalyze the addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Probably acts by adding O-linked sugars to yet unknown proteins (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the glycosyltransferase 41 family. O-GlcNAc transferase subfamily.


FUNCTION: Prenyltransferase; part of the gene cluster that mediates the biosynthesis of dibenzodioxocinones such as pestalotiollide B, a novel class of inhibitors against cholesterol ester transfer protein (CEPT) (PubMed:31474098). The biosynthesis initiates from condensation of acetate and malonate units catalyzed by the non-reducing PKS pks8/GME11356. Pks8/GME11356 lacks a thioesterase (TE) domain, which is important to the cyclizing of the third ring of atrochrysone carboxylic acid, and the esterase GME11355 might play the role of TE and catalyzes the cyclization reaction of the C ring. The lactamase-like protein GME11357 (or other beta-lactamases in Pestalotiopsis microspora) probably hydrolyzes the thioester bond between the ACP of pks8/GME11356 and the intermediate to release atrochrysone carboxylic acid, which is spontaneously dehydrates to form endocrocin anthrone. Endocrocin anthrone is further converted to emodin via the endocrocin intermediate. Emodin is then oxidized by several enzymes such as the Baeyer-Villiger oxidase GME11358, the oxidoreductase GME11367, the short chain dehydrogenase/reductase GME11373, as well as by other oxidoreductases from the cluster, to modify the A and C rings and open the B ring, and finally yield monodictyphenone. The prenyltransferase GME11375 may catalyze the addition reaction between the C5 side chains and the carbon bone of dibenzodioxocinones. The remaining biochemical reactions to the final product dibenzodioxocinones should be methylation catalyzed by methyltransferase GME11366 and reduction and lactonization reaction catalyzed by a series of oxidordeuctases (Probable). SIMILARITY: Belongs to the tryptophan dimethylallyltransferase family.


FUNCTION: Snake venom phospholipase A2 (PLA2) that exhibits medium anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC(50) is 90 nM). PLA2 catalyzes the calcium- dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the phospholipase A2 family. Group II subfamily. D49 sub-subfamily.


FUNCTION: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the arylamine N-acetyltransferase family.


SIMILARITY: Belongs to the TMEM121 family.


FUNCTION: Involved in the transfer of copper ion to the apo form O- aminophenol oxidase GriF in the grixazone biosynthetic pathway. SIMILARITY: Belongs to the melC1 family.


FUNCTION: Class-II terpene synthase that synthesizes 8-hydroxy-copalyl diphosphate. Involved in the biosynthesis of cis-abienol, a labdane diterpene that can be used as synthesis precursor of ambergris substitution fragance products. SUBCELLULAR LOCATION: Plastid, chloroplast. SIMILARITY: Belongs to the terpene synthase family.


FUNCTION: Methyltransferase that performs automethylation (By similarity). No other methyl-accepting substrate has been identified yet (By similarity). Component of the velvet transcription factor complex that acts as a global regulator for secondary metabolite gene expression (PubMed:23390613, PubMed:24909838). Required for the expression of VEL1 (PubMed:23390613). Regulates expression of the carbohydrate-active enzyme gene clusters, but does not act by directly modulating H3K4 or H3K9 methylation (PubMed:22554051). Required for conidiation (PubMed:22554051). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the methyltransferase superfamily. LaeA methyltransferase family.


FUNCTION: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3 (By similarity). SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the natriuretic peptide family.


FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. SIMILARITY: Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.


FUNCTION: Required for endoplasmic reticulum integrity. Regulates the distribution of TOR1A between the endoplasmic reticulum and the nuclear envelope as well as induces TOR1A, TOR1B and TOR3A ATPase activity. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane. SIMILARITY: Belongs to the TOR1AIP family.


FUNCTION: Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self- renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. FUNCTION: [Isoform 2]: Acts as a regulator of myogenesis in cooperation with HDGFL2 (PubMed:32459350). Mediates the interaction of HDGFL2 with the BAF complex (PubMed:32459350). HDGFL2-DPF3a activate myogenic genes by increasing chromatin accessibility through recruitment of SMARCA4/BRG1/BAF190A (ATPase subunit of the BAF complex) to myogenic gene promoters (PubMed:32459350). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the requiem/DPF family.


FUNCTION: Essential cell division protein. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein Note=Localizes to the division septum. SIMILARITY: Belongs to the FtsQ/DivIB family. FtsQ subfamily.


FUNCTION: May play an important role in cardiac development and function. May regulate cardiac conduction and the function of the gap junction protein GJA1. May contribute to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (PubMed:26403541). Regulates mitochondrial respiration and mitochondrial DNA copy number maintenance (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein Mitochondrion inner membrane; Multi-pass membrane protein Note=Localizes at the intercalated disk in ventricular tissue and cardiomyocytes.


FUNCTION: Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. SIMILARITY: Belongs to the profilin family.


FUNCTION: Required for required for normal vacuolar morphology and for vacuolar protein transport. Also required for endosome-to-Golgi protein transport. SUBCELLULAR LOCATION: Endosome membrane; Multi-pass membrane protein.


FUNCTION: Probable regulator of exocrine pancreas development. SIMILARITY: Belongs to the PPDPF family.


FUNCTION: DNA-dependent RNA polymerase that catalyzes the transcription of viral genes. SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase family.


FUNCTION: Transcriptional repressor that binds to the promoter region of Mpv17l isoform M-LP short and regulates its age-dependent and heat- induced expression (PubMed:20231359). By regulating Mpv17l expression, contributes to the regulation of genes involved in H(2)O(2) metabolism and the mitochondrial apoptotic cascade (PubMed:20231359) (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.


FUNCTION: Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the CC2D1 family.


FUNCTION: Catalyzes the addition of the first of nine mannose moieties to form a dolichol-lipid linked oligosaccharide intermediate required for proper N-linked glycosylation. SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type II membrane protein. SIMILARITY: Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.


FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions. SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.


FUNCTION: NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L- methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2- thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes. SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. SIMILARITY: Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.


FUNCTION: Part of the ABC transporter complex PhnCDE involved in phosphonates import. Responsible for energy coupling to the transport system. SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. SIMILARITY: Belongs to the ABC transporter superfamily. Phosphonates importer (TC 3.A.1.9.1) family.


FUNCTION: A P subtype restriction enzyme that recognizes the double- stranded sequence 5'-GGNNCC-3' and cleaves after N-3.


FUNCTION: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes (By similarity). SUBCELLULAR LOCATION: Mitochondrion. SIMILARITY: Belongs to the prokaryotic/mitochondrial release factor family. Mitochondrion-specific ribosomal protein mL62 subfamily.


FUNCTION: Omega-agatoxins are antagonists of voltage-gated calcium channels (Cav). Causes rapid general flaccid paralysis followed by death in 10-30 minutes when injected in mice at dose levels of 5 ug per mouse. SUBCELLULAR LOCATION: Secreted. SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 03 (type II/III omega-agtx) subfamily.


SIMILARITY: Belongs to the prickle / espinas / testin family.


SUBCELLULAR LOCATION: Plastid, chloroplast. SIMILARITY: Belongs to the bacterial ribosomal protein bL33 family.


FUNCTION: Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex. Also involved in the regulation of telomere length. SUBCELLULAR LOCATION: Nucleus. SIMILARITY: Belongs to the IES6 family.


SIMILARITY: Belongs to the FBPase class 3 family.


FUNCTION: May regulate immune response to the intracellular capsid in acting as a T-cell tolerogen, by having an immunoregulatory effect which prevents destruction of infected cells by cytotoxic T-cells. This immune regulation may predispose to chronicity during perinatal infections and prevent severe liver injury during adult infections. SUBCELLULAR LOCATION: Secreted Host nucleus. SIMILARITY: Belongs to the orthohepadnavirus precore antigen family.


SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase family.


FUNCTION: Ribosome-bound, Hsp70-type chaperone that assists in the cotranslational folding of newly synthesized proteins in the cytosol. Stimulates folding by interacting with nascent chains, binding to short, largely hydrophobic sequences exposed by unfolded proteins, thereby stabilizing longer, more slowly translated, and aggregation- prone nascent polypeptides and domains that cannot fold stably until fully synthesized. The Hsp70-protein substrate interaction depends on ATP-binding and on allosteric regulation between the NBD and the SBD. The ATP-bound state is characterized by a fast exchange rate of substrate (low affinity state), while in the ADP-bound state exchange is much slower (high affinity state). During the Hsp70 cycle, the chaperone switches between the ATP-bound state (open conformation) and the ADP-bound state (closed conformation) by major conformational rearrangements involving mainly the lid domain. Ssb cooperates with a specific Hsp40/Hsp70 co-chaperone termed the ribosome-associated complex (RAC), which stimulates the ATPase activity of the ribosome- associated pool of Ssbs and switches it to the high affinity substrate binding state. Hsp110 chaperone SSE1 and FES1 act as nucleotide exchange factors that cause substrate release. SUBCELLULAR LOCATION: Cytoplasm Note=Associated with translating ribosomes. SIMILARITY: Belongs to the heat shock protein 70 family. Ssb-type Hsp70 subfamily.


SIMILARITY: Belongs to the NUT family.


FUNCTION: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Probable component of kinase complex containing nonC and recruited to stalled ribosomes (By similarity). SIMILARITY: Belongs to the SMG8 family.


FUNCTION: O-acyltransferase; part of the gene cluster that mediates the biosynthesis of calidodehydroaustin, a fungal meroterpenoid (PubMed:28233494, PubMed:29076725). The first step of the pathway is the synthesis of 3,5-dimethylorsellinic acid by the polyketide synthase ausA (PubMed:28233494). 3,5-dimethylorsellinic acid is then prenylated by the polyprenyl transferase ausN (PubMed:28233494). Further epoxidation by the FAD-dependent monooxygenase ausM and cyclization by the probable terpene cyclase ausL lead to the formation of protoaustinoid A (By similarity). Protoaustinoid A is then oxidized to spiro-lactone preaustinoid A3 by the combined action of the FAD-binding monooxygenases ausB and ausC, and the dioxygenase ausE (By similarity). Acid-catalyzed keto-rearrangement and ring contraction of the tetraketide portion of preaustinoid A3 by ausJ lead to the formation of preaustinoid A4 (By similarity). The aldo-keto reductase ausK, with the help of ausH, is involved in the next step by transforming preaustinoid A4 into isoaustinone which is in turn hydroxylated by the P450 monooxygenase ausI to form austinolide (By similarity). The cytochrome P450 monooxygenase ausG modifies austinolide to austinol (By similarity). Austinol is further acetylated to austin by the O- acetyltransferase ausP, which spontaneously changes to dehydroaustin (PubMed:28233494). The cytochrome P450 monooxygenase ausR then converts dehydroaustin is into 7-dehydrodehydroaustin (PubMed:28233494). The hydroxylation catalyzed by ausR permits the O-acetyltransferase ausQ to add an additional acetyl group to the molecule, leading to the formation of acetoxydehydroaustin (PubMed:28233494). The short chain dehydrogenase ausT catalyzes the reduction of the double bond present between carbon atoms 1 and 2 to convert 7-dehydrodehydroaustin into 1,2-dihydro-7-hydroxydehydroaustin (PubMed:28233494). AusQ catalyzes not only an acetylation reaction but also the addition of the PKS ausV diketide product to 1,2-dihydro-7-hydroxydehydroaustin, forming precalidodehydroaustin (PubMed:28233494). Finally, the iron/alpha- ketoglutarate-dependent dioxygenase converts precalidodehydroaustin into calidodehydroaustin (PubMed:28233494). SIMILARITY: Belongs to the plant acyltransferase family.


FUNCTION: Acts in maintaining the cortical microtubules organization essential for anisotropic cell growth. SIMILARITY: Belongs to the SPIRAL1 family.


FUNCTION: Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation. Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates. Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN- beta production. Recruits also A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways. Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling. As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis. Upon NBR1 recruitment to the SQSTM1- ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation. SUBCELLULAR LOCATION: Cytoplasm Mitochondrion Preautophagosomal structure Cytoplasmic vesicle, autophagosome.


FUNCTION: May catalyze the ATP-dependent phosphorylation of lipids other than diacylglycerol (DAG). In fact, is not able to exhibit diacylglycerol kinase activity in vitro. SIMILARITY: Belongs to the diacylglycerol/lipid kinase family.


FUNCTION: Heme transporter that regulates intracellular heme availability through the endosomal or lysosomal compartment (PubMed:18418376). In macrophages of the reticuloendothelial system, is the heme transporter for heme-iron recycling. Essential for macrophage iron homeostasis, transports heme from the phagolysosome to the cytoplasm during erythrophagocytosis (EP) (PubMed:23395172). SUBCELLULAR LOCATION: Endosome membrane; Multi-pass membrane protein Lysosome membrane; Multi-pass membrane protein Cytoplasmic vesicle, phagosome membrane; Multi-pass membrane protein Note=In macrophages, specifically localizes to the phagolysosomal membranes during erythrophagocytosis. SIMILARITY: Belongs to the HRG family.


FUNCTION: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain. SUBCELLULAR LOCATION: [Isoform 2]: Secreted. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.


FUNCTION: Catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation of sulfur-containing amino acids. SUBCELLULAR LOCATION: Mitochondrion intermembrane space.


FUNCTION: mRNA-binding protein required for maternal mRNA storage, translation and degradation during oocyte maturation (PubMed:27913641). Probably promotes formation of some phase-separated membraneless compartment that stores maternal mRNAs in oocytes: acts by undergoing liquid-liquid phase separation upon binding to maternal mRNAs (By similarity). Binds to the 3'-UTR of zona pellucida mRNAs, inhibiting their translation (PubMed:27913641). SUBCELLULAR LOCATION: Cytoplasm, Cytoplasmic ribonucleoprotein granule. SIMILARITY: Belongs to the ZAR1 family.


FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. SIMILARITY: Belongs to the diphthine synthase family.


SUBCELLULAR LOCATION: Bacterial flagellum basal body. SUBCELLULAR LOCATION: Bacterial flagellum basal body. SIMILARITY: Belongs to the flagella basal body rod proteins family.


FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. SIMILARITY: Belongs to the complex I 49 kDa subunit family.


SIMILARITY: Belongs to the eukaryotic ribosomal protein eL20 family.


FUNCTION: Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity). FUNCTION: Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation. SIMILARITY: Belongs to the universal ribosomal protein uS3 family.


FUNCTION: Transcriptional activator of glycolytic gene expression, such as enolase genes (ENO1 and ENO2), glyceraldehyde-3-phosphate dehydrogenase gene (TDH), phosphoglycerate kinase (PGK1), phosphoglycerate mutase (PGM1), pyruvate kinase (PYK1) and triosephosphate isomerase (TPI1) genes. Binds DNA on E-box motifs: 5'- CANNTG-3'. SUBCELLULAR LOCATION: Nucleus.