Blame for data/Supplementary_Data/Supplementary_Data_1_expert_curation.csv

"Entry","UniProt_ID","Length_aa","p_JAY","Retained","Judge_label","Curator_score","Specificity","Information","Truncated","SwissProt_description","ProtTale_prediction","Curator_notes"
"1","TOS1_CANAL","467","0.2486","no","Yes","4","Equally specific","Less informative","no","Probable circularly permuted 1,3-beta-glucanase involved in cell wall modification through beta-1,3-glucan network alterations such as increased branching or remodeling. Plays a role in engulfment by host macrophages.","Secreted beta-glucanase involved in the degradation of plant cell wall polysaccharides. Hydrolyzes 1,3- and 1,4-beta-glucans.","Swissprot describes cell wall modification by branching/remodeling, which is reinforcement. Prediction describes degradation."
"2","FUMC_SCHMA","488","0.2159","no","not-Yes","2","Equally specific","Less informative","no","Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism.","Catalyzes both the decarboxylation of 2-oxoglutarate and the formation of succinate from oxaloacetate, generating pyruvate and CO(2).","Both describe TCA-cycle intermediates, but no shared mechanism."
"3","OXDA_RASEM","368","0.2575","no","Yes","5","Equally specific","Less informative","no","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients. Unusually, has high activity on D-glutamate.","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Same description, but ""Unusually, has high activity on D-glutamate."" in Swissprot only"
"4","BLC14_ECOLX","291","0.2286","no","Yes","4","Equally specific","Less informative","no","Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, second, and third- generation cephalosporins. Has cefotaxime-hydrolyzing activity.","Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first and second- generation cephalosporins. Has penicillinase activity.","""Has cefotaxime-hydrolyzing activity."" Swissprot, ""Has penicillinase activity."" prediction. Prediction omits 3rd gen activity."
"5","FAE1_PENPR","280","0.2713","no","not-Yes","2","More specific","More informative","yes","Involved in degradation of plant cell walls. Hydrolyzes the feruloyl-arabinose ester bond in arabinoxylans, and the feruloyl- galactose ester bond in pectin.","Secreted lipase involved in Dandruff and seborrheic dermatitis (D/SD) probably via lipase-mediated breakdown of sebaceous lipids and release of irritating free fatty acids. Shows activity against monoglyceride and diglyceride substrates but not triolein. Able to cleave oleic acid from monoolein and diolein, and oleic acid from triolein. Able to hydrolyze p-nitrophenyl palmitate and p-nitrophenyl laurate, but not p-nitrophenyl palmitoleate or p-nitrophenyl stearate. Able to cleave the ester bonds of olive oil","Both describe hydrolases, but different types (feruloyl esterase vs. lipase), pathways, ."
"6","OXDD_OCTVU","336","0.2611","no","not-Yes","3","Less specific","Less informative","no","Selectively catalyzes the oxidative deamination of acidic amino acids. Suppresses the level of D-aspartate in the brain, an amino acid that can act as an agonist for glutamate receptors. Protects the organism from the toxicity of D-amino acids. May also function in the intestine.","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Swissprot describes ""selective"" catalysis, prediction describes ""broad substrate specificity"""
"7","CUBA_PSICU","343","0.1666","no","Yes","5","Equally specific","Less informative","yes","Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to various sesquiterpenes, including cubebol as the major product and beta-copaene, delta-cadinene, germacrene D, germacrene D-4-ol, sativene, tau-muurolol, beta-cubebene and beta- elemene as minor compounds, along with minute amounts of further sesquiterpenoids.","Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to various sesquiterpenes, including alpha-copaene, beta-copaene, beta-caryophyllene, gamma-cadinene, delta-cadinene, alpha-humulene, beta-selinene, alpha-cadinol, alpha-cadinol-9-ol, alpha-cadinol-10-ol, alpha-cadinol-11-ol, alpha-cadinol-12-ol, alpha-cadinol-13-ol, alpha-cadinol-1","Swissprot describes major product, prediction does not. Both describe sesquiterpenes."
"8","E2609_XANAC","431","0.2612","no","not-Yes","2","More specific","More informative","no","Effector protein that targets host peptidoglycan and acts as a peptidoglycan hydrolase. It contributes to direct killing of adjacent bacterial cells, thereby providing a competitive growth advantage for Xanthomonas in mixed bacterial communities.","Pore-forming component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria. The C-terminal 250 amino acids of this protein form a pore that allows passive diffusion of small molecules through the membrane. The full-length protein also has GTPase-activating protein (GAP) activity.","Both describe effector proteins, and agree on biological role. Swissprot describes peptidogylcan hydrolase, prediction describes CDI effector."
"9","GUAB1_MYCS2","478","0.166","no","not-Yes","3","Equally specific","Equally informative","no","Involved in the purine-salvage pathway. Catalyzes the NADPH-dependent conversion of GMP to IMP. Is not essential for viability, but may contribute to the regulation of the purine nucleotide pool by recycling GMP to IMP.","Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.","Both describe nucleotide salvage pathway catalysis, but different roles."
"10","AGLTA_ASPNC","555","0.1926","no","Yes","5","Equally specific","Less informative","yes","Transfers a segment of a (1->4)-alpha-D-glucan to an acceptor oligosaccharide; maltose and larger maltooligosaccharides are the most efficient acceptor substrates, though it also has some activity on alpha(1,3)-linked oligosaccharides. Also has a low hydrolyzing activity on starch, producing short oligosaccharides. Contributes to cell wall polysaccharide biosynthesis and cell wall assembly.","Transfers a segment of a (1->4)-alpha-D-glucan to an acceptor oligosaccharide; can transfer from alpha-1,3-1,4-1,4-1,4-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-1,6-","Prediction incomplete? Swissprot description contains more detail, and prediction seems incomplete. However, both describe exact same core function."
"11","AGLTB_ASPNC","549","0.2245","no","not-Yes","2","Equally specific","Less informative","no","Transfers a segment of a (1->4)-alpha-D-glucan to an acceptor oligosaccharide; maltose and larger maltooligosaccharides are the most efficient acceptor substrates, though it also has very limited activity on alpha(1,3)-linked oligosaccharides. Also has a low hydrolyzing activity on starch, producing short oligosaccharides. Contributes to cell wall polysaccharide biosynthesis and cell wall assembly.","Cleaves the terminal alpha 1,4-linked mannose residue from high-mannose type oligosaccharides present on glycoprotein and glycolipid substrates. Involved in the biosynthesis of high-mannose type oligosaccharides (Man(5-9)GlcNAc(2)) present in N- and O-linked glycoproteins.","Both act on oligosaccharides. Swissprot describes transfer, prediction describes cleavage."
"12","DMDC_ROSNI","591","0.2788","no","not-Yes","3","Equally specific","Equally informative","yes","Involved in the assimilation of dimethylsulphoniopropionate (DMSP), an important compound in the fixation of carbon in marine phytoplankton, by mediating the conversion of 3-(methylthio)propanoyl- CoA (MMPA-CoA) to 3-(methylthio)acryloyl-CoA (MTA-CoA).","Acyl-CoA dehydrogenase that catalyzes the dehydrogenation of 3-hydroxybutyryl-CoA to butyryl-CoA. Is also able to catalyze the dehydrogenation of butyryl-CoA, propionyl-CoA and isovaleryl-CoA, but with lower efficiency. Catalyzes the dehydrogenation of short-chain acyl-CoAs with decreasing efficiency in the following order: 3-hydroxybutyryl-CoA > butyryl-CoA > propionyl-CoA > isovaleryl-CoA > valeryl-CoA > acetoacetyl-CoA = acetyl-CoA","Shared mechanism (acyl-CoA dehydrogenation), but no substrate overlap."
"13","CPSKS_PHYPA","881","0.1576","no","Yes","3","More specific","Less informative","yes","Bifunctional copalyl diphosphate/kaurene synthase involved in the biosynthesis of labdane-related diterpenoids (LRDs) natural products such as ent-beyerene, an antimicrobial compound. Supports the conversion of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CDP). Also catalyzes the subsequent cyclization of ent-CDP into many diterpenes, including ent-kaur-16-ene as the major product, and ent-beyerene, ent-sandaracopimaradiene, ent-kaur-15-ene (ent- isokaurene) and 16-hydroxy-ent-kaurene (ent-16-alpha-hydroxy-kaurene) as minor products.","Involved in the biosynthesis of ent-kaurene diterpenoids natural products such as miltiradiene, miltiradien-10-ol, miltiradien-10-yl acetate, miltiradien-10-ol acetate, miltiradiene and miltiradien-10-ol acetate, which exhibit a range of biological activities including antimicrobial (Probable). Miltiradiene is probably converted to miltiradien-10-ol and miltiradien-10-yl acetate by a cytochrome P450 monooxygenase (Probable","Both describe diterpene synthase, products with antimicrobial relevance, and the same diterpenoid superfamily. They describe different specific branches."
"14","CP260_SORC5","394","0.1662","no","not-Yes","3","Less specific","Less informative","no","Hydroxylase that can catalyze the in vitro conversion of the sesquiterpenoid nootkatone, a natural organic compound produced by some plants, to at least five hydrophilic products. The native ferredoxin reductase FdR_B and either Fdx2 or Fdx8 ferredoxins can act as the redox partners for the conversion of nootkatone.","Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.","Prediction describes a less specific enzyme of the same class and function."
"15","IYD_THENN","186","0.227","no","not-Yes","1","Equally specific","Equally informative","no","Catalyzes the dehalogenation of halotyrosines such as 3- bromo-L-tyrosine, 3-chloro-L-tyrosine, 3-iodo-L-tyrosine and 3,5- diiodo-L-tyrosine. Activity towards 2-iodophenol is weak.","Transforms 4-hydroxy-2-ketovalerate to (4R)-4-hydroxy-4-methyl-2- oxopentanoate [FAD-dependent]. Can also convert (4R,S)-4-hydroxy-4-methyl-2- oxopent-2-enoic acid to 4-hydroxy-4-methyl-2,4-pentadienoic acid [FAD-dependent]. Cannot use pyruvate as substrate.",""
"16","OXDD_TALTH","370","0.1803","no","Yes","3","Less specific","Equally informative","no","Selectively catalyzes the oxidative deamination of acidic amino acids. Protects the organism from the toxicity of D-amino acids. Enables the organism to utilize D- amino acids as a source of nutrients.","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Swissprot describes ""selective"" catalysis, prediction describes ""broad substrate specificity"""
"17","OXDD_RAT","341","0.2634","no","not-Yes","3","Less specific","Less informative","no","Selectively catalyzes the oxidative deamination of acidic amino acids. Suppresses the level of D-aspartate in the brain, an amino acid that can act as an agonist for glutamate receptors. Protects the organism from the toxicity of D-amino acids. May also function in the intestine.","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Swissprot describes ""selective"" catalysis, prediction describes ""broad substrate specificity"""
"18","CYS_STREN","367","0.1951","no","not-Yes","1","Equally specific","Less informative","no","Diterpene synthase that catalyzes the conversion of geranylgeranyl diphosphate (GGPP) to cattleyene. Cannot use geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylfarnesyl diphosphate (GFPP).","Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) into (+)-delta-cadinene via a 1,10-cyclization.","Swissprot description explicitly contradicts prediction (CANNOT use FPP according to Swissprot, converts FPP accordign to prediction)."
"19","DHOM_SULTO","304","0.2446","no","Yes","5","Equally specific","Equally informative","no","Catalyzes the conversion of L-aspartate-beta-semialdehyde (L- Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of threonine and methionine from aspartate.","Catalyzes the conversion of L-aspartate-beta-semialdehyde (L- Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of threonine and methionine from aspartate.","Exact same description."
"20","PUR8_SCHMA","480","0.2625","no","not-Yes","1","Equally specific","Equally informative","no","Catalyzes conversion of succinyladenosine monophosphate (SAMP) to AMP and fumarate on the purine salvage pathway.","Catalyzes the transfer of an acetyl group from acetyl-CoA to L-aspartate to produce 4-acetyl-L- aspartate and ATP. Can also catalyze the transfer of an acetyl group from acetyl-CoA to D-aspartate to produce 4-acetyl-D-aspartate and 2 ATP.",""
"21","AAE1_CANSA","720","0.2692","no","not-Yes","2","More specific","Less informative","no","Involved in the biosynthesis of cannabinoid-related terpenophenolic natural products, which have pharmacological activity. Acyl-activating enzyme that catalyzes the conversion of hexanoic acid to hexanoyl-CoA, precursor of the cannabinoid pathway. Can also activate other fatty acids including heptanoate, octanoate and nonanoate.","Catalyzes the formation of 2-oxoadipate-4-phosphate (OAP) from acetyl-CoA and ATP. Also catalyzes the ATP-dependent synthesis of 2-oxoadipate from malonyl-CoA and ATP.","Both are CoA synthases, but disagree on substrates and products."
"22","B12GT_IGNAJ","716","0.2825","no","not-Yes","1","Equally specific","Less informative","yes","Glycosyltransferase acting on beta-1,2-glucooligosaccharides. Catalyzes the transfer of a glucosyl residue from the non-reducing end of a 1,2-beta-D-glucan to a glucose residue of an acceptor molecule, forming a beta-1,2-glucosidic bond. The beta-1,2-linked glucose dimer sophorose is the preferred donor in vitro. Has a very broad specificity for the acceptor and can act on various aryl- and alkyl- glucosides. Does not show any hydrolytic activity.","Catalyzes the hydrolysis of terminal (1->4)-beta-D-galactosyl residues in beta-D-galactosides, galactomannans and galactooligosaccharides (GOS) to produce D-galactose and D-galactobiose, which can be used as substrates by the colonic microbiota. Gets its name from its ability to hydrolyze galacto-alpha-1,4-D-galactose (lacto-N-biose), galacto-alpha-1,3-D-galactose (lacto-N-triose) and galacto","Both describe cabohydrate-active enzymes but contain an explicit contradiction."
"23","CGDA_EUBC6","290","0.1436","no","not-Yes","1","More specific","Equally informative","no","Carbon-carbon bond-cleaving enzyme which participates in the metabolism of C-glycosides. Acts on the C6-glycosylated compounds 3''-dehydroisoorientin (3''-oxo- homoorientin) and 3''-dehydroisovitexin (3''-oxo-isovitexin).","Involved in the catabolism of D-xylose, a major component of hemicelluloses. Catalyzes the dehydration of D-xylulose-5-phosphate to form 2-dehydro-3-deoxy-D-arabino-heptose (DAH).",""
"24","DHOM_PYRHO","319","0.2795","no","Yes","5","Equally specific","Less informative","no","Catalyzes the conversion of L-aspartate-beta-semialdehyde (L- Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of threonine and methionine from aspartate. Utilizes NADH but not NADPH as coenzyme.","Catalyzes the conversion of L-aspartate-beta-semialdehyde (L- Asa) to L-homoserine (L-Hse), the third step in the biosynthesis of threonine and methionine from aspartate.","Same description, but Swissprot includes an additional description ""Utilizes NADH but not NADPH as coenzyme."""
"25","CAPV_PSEU5","333","0.2892","no","not-Yes","2","More specific","Less informative","no","Effector phospholipase of a CBASS antiviral system. CBASS (cyclic oligonucleotide-based antiphage signaling system) provides immunity against bacteriophages. The CD- NTase protein (CdnA) synthesizes cyclic nucleotides in response to infection; these serve as specific second messenger signals. The signals activate a diverse range of effectors, leading to bacterial cell death and thus abortive phage infection. A type II-A(GA) CBASS system.","Probable lipolytic enzyme involved in the biosynthesis of the antibiotic polyketide bacillaene. Catalyzes the hydrolysis of the ester bond of the polyketide synthase (PKS)- derived (R)-3-hydroxymyristoyl-ACP by releasing (R)-3-hydroxymyristoyl-CoA.",""
"26","LAN1_PYRDE","540","0.2089","no","not-Yes","3","Less specific","Equally informative","yes","Sterol 14-alpha demethylase; part of the gene cluster that mediates the biosynthesis of tetrahydropyranyl antifungal agent lanomycin that acts as an inhibitor of CYP51 and blocks the ergosterol biosynthesis. Sterol 14-alpha-demethylase plays a critical role in the biosynthesis of ergosterol, the major sterol component in fungal membranes that participates in a variety of functions. Acts as a self-resistant CYP51 that contains mutations found in CYP51s isolated from azole resistance strains and that is not inhibited by the final product of the cluster, lanomycin.","Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of the indole diterpenes penitrems. Within the pathway, PC-2 catalyzes the oxidation of penitrem D to penitrem E. The geranylgeranyl diphosphate (GGPP) synthase penG catalyzes the first step in penitrem biosynthesis via conversion of farnesyl pyrophosphate and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP). Condensation of indole-3-glycerol phosphate with GGPP by penC then forms 3-geranylgeranylindole (3-","Both belong to Cytochrome P450 superfamily, but act on different substrates."
"27","OASD_RHOJR","490","0.1719","no","not-Yes","1","Equally specific","Equally informative","no","Dehydrogenase involved in the degradation of steroids. Catalyzes the introduction of a double bond between the C4 and C5 atoms of 3-keto-(5alpha)-steroids. Catalyzes the conversion of 5alpha- androst-1-ene-3,17-dione to androsta-1,4-diene-3,17-dione, and probably the conversion of 5alpha-androstan-3,17-dione to androst-4-ene-3,17- dione.","Involved in the degradation of 3-methylglutaconic acid. Catalyzes the retro-aldol cleavage of 3- methylglutaconyl-CoA to form (3R)-2-hydroxyisovalerate and acetoacetyl-CoA. Also able to catalyze the retro- aldol cleavage of 2-hydroxyisocaproyl-CoA to form (3R)-2-hydroxyisocaproate and acetoacetyl-CoA.",""
"28","TSOXA_CORS9","965","0.2411","no","not-Yes","1","Equally specific","Less informative","no","In the presence of tetrahydrofolate, catalyzes the oxidative demethylation of sarcosine to yield glycine, 5,10- methylenetetrahydrofolate and hydrogen peroxide. In the absence of tetrahydrofolate, catalyzes the oxidative demethylation of sarcosine to yield glycine, formaldehyde and hydrogen peroxide. Can also use N- methyl-L-alanine and N-ethyl-L-glycine. Is very specific for oxygen as an acceptor.","Catalyzes the dehydration of trans-3-hydroxy-L-proline (t3LHyp) and trans-4-hydroxy-L-proline (t4LHyp) to Delta(1)-pyrroline-2-carboxylate (Pyr2C). Also acts on cis-3-hydroxy-L-proline (c3LHyp) and cis-4-hydroxy-L-proline (c4LHyp).",""
"29","TSOXB_CORS9","405","0.0931","no","not-Yes","2","Equally specific","Less informative","no","In the presence of tetrahydrofolate, catalyzes the oxidative demethylation of sarcosine to yield glycine, 5,10- methylenetetrahydrofolate and hydrogen peroxide. In the absence of tetrahydrofolate, catalyzes the oxidative demethylation of sarcosine to yield glycine, formaldehyde and hydrogen peroxide. Can also use N- methyl-L-alanine and N-ethyl-L-glycine. Is very specific for oxygen as an acceptor.","The cytochrome b551 component of the succinate dehydrogenase (SDH) complex catalyzes the transfer of electrons from succinate to the flavoprotein subunit SDH of the complex. The complex is involved in the electron transfer between the mitochondrial respiratory chain and the oxidative phosphorylation system.","Both are involved in oxidation-reduction pathways."
"30","CAPDH_SYNY3","398","0.208","no","not-Yes","3","Equally specific","Less informative","no","Dehydrogenase involved in the biosynthesis of spermidine via the carboxyaminopropylagmatine (CAPA) pathway. Catalyzes the reductive condensation of agmatine and L-aspartate-beta- semialdehyde (ASA) into CAPA. Shows activity toward putrescine and 1,3-diaminopropane, but the catalytic efficiency is three to four orders of magnitude lower than that for agmatine. Cannot use cadaverine or spermidine.","Involved in the degradation of (S)-2,3-butanediol. Catalyzes the NAD(P)H-dependent reduction of 4-hydroxybenzoate (4HB) to 1,2-dihydroxy-3-methylbutanoate (DHMB). Can also use 3-hydroxybenzoate (3HB) and 2-hydroxybenzoate (2HB) as substrates.","Both describe dehydrogenases with the same co-factors, but different roles in unrelated pathways."
"31","CLS_TRYB2","639","0.2365","no","not-Yes","3","Equally specific","Less informative","no","Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. CL is a key phospholipid in mitochondrial membranes and plays important roles in maintaining the functional integrity and dynamics of mitochondria under both optimal and stress conditions.","Involved in phosphatidylglycerol biosynthesis. Catalyzes the transfer of the phosphatidylglycerol moiety from phosphatidylglycerol phosphate (PGP) to the 1-OH group of the sn-2 acyl group of glycerol-3-phosphate.","Both describe phosphatidyl group transfer in glycerophospholipid biosynthesis, but not the same part of the pathway."
"32","ACAD9_DROME","639","0.2986","no","not-Yes","2","More specific","Equally informative","no","As part of the MCIA complex, primarily participates in the assembly of the mitochondrial complex I and therefore plays a role in oxidative phosphorylation.","Catalyzes the oxidation of malate to oxaloacetate. May play a role in glucose and fatty acid metabolism, as well as in the antioxidant response.",""
"33","FAD1_CANGA","304","0.293","no","not-Yes","1","Equally specific","Equally informative","no","Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.","Catalyzes the synthesis of L-glutamate from 2-oxoglutarate and 2 molecules of ATP.",""
"34","THS_METMP","543","0.2673","no","Yes","3","Less specific","Equally informative","no","Molecular chaperone; binds unfolded polypeptides and stimulates protein folding in a nucleotide-dependent manner. Cooperative ATP hydrolysis in each ring closes the lid, which is required for substrate folding. ATP and AMP-PNP allow substrate refolding but ADP does not. Hydrolyzes CTP as well as ATP; UTP and GTP are poorer substrates.","Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.","Both describe molecular chaperones and cooperative ATP hydrolysis, but describe different contexts."
"35","PHYA_PENOX","461","0.2391","no","not-Yes","3","Equally specific","Equally informative","no","Catalyzes the phosphate monoester hydrolysis of phytic acid (myo-inositol hexakisphosphate), which results in the stepwise formation of myo-inositol pentakis-, tetrakis-, tris-, bis-, and monophosphates, as well as the liberation of inorganic phosphate. Myo-inositol 2-monophosphate is the end product.","Hydrolyzes phosphodiester bonds of nucleoside 5'-phosphates, yielding nucleoside 5'-monophosphates and a free purine or pyrimidine nucleoside. Prefers inosine 5'- monophosphate (IMP) to adenosine 5'-monophosphate (AMP), guanosine 5'-monophosphate (GMP) and cytidine 5'-monophosphate (cGMP) as substrates.","Both are hydrolases, but cleave different bonds."
"36","AURH_STRTU","406","0.149","no","not-Yes","3","Equally specific","Equally informative","no","Bifunctional cytochrome P450 protein involved in the biosynthesis of the antibiotic aureothin, a nitroaryl polyketide metabolite with antifungal, cytotoxic and insecticidal activities. Catalyzes the hydroxylation of luteothin (also called deoxyaureothin), leading to the formation of the intermediate (7R)-7-hydroxydeoxyaureothin, followed by the formation of the aureothin tetrahydrofuran ring, the final step in the biosynthesis of aureothin.","Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vitro, catalyzes the hydroxylation at position C-10 of 10-deoxycarminomycin and 10-deoxydaunorubicin to yield 10-hydroxycarminomycin and 10-hydroxydaunorubicin, respectively.","Both are Cytochrome P450 monooxygenases, but describe different roles, substrates, and products."
"37","PHYA_NEUCR","509","0.1652","no","not-Yes","2","Less specific","Less informative","no","Catalyzes the phosphate monoester hydrolysis of phytic acid (myo-inositol hexakisphosphate), which results in the stepwise formation of myo-inositol pentakis-, tetrakis-, tris-, bis-, and monophosphates, as well as the liberation of inorganic phosphate. Myo-inositol 2-monophosphate is the end product. Is also able to dephosphorylate the classic acid phosphatase substrate p-nitrophenyl phosphate.","Catalyzes the hydrolysis of inorganic polyphosphate (polyP) chains of many hundreds of phosphate residues to shorter lengths. Can use a broad spectrum of polyP substrates from a few hundreds of phosphate residues to at least 2000 phosphate residues.","Both are hydrolases that release phosphate, but different substrate and bond chemistry."
"38","DAO_GLUPR","326","0.2059","no","Yes","5","Equally specific","More informative","no","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity.","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Same description, but prediction contains additional statement on broader role."
"39","HYLB_ENTFA","1004","0.2797","no","Yes","3","Equally specific","Equally informative","no","Degrades hyaluronic acid (HA) and chondroitin sulfate (CS) A in vitro. Is not active against heparin sodium salt (HS). Involved in the pathogenesis of vancomycin-resistant E.faecalis infections. Contributes to attenuation of the lipopolysaccharide (LPS)-mediated nuclear factor (NF)-kappa-B activation assayed in the mouse RAW-Blue reporter macrophages.","Degrades glycosaminoglycans (GAGs) such as chondroitin-4- sulfate, dermatan sulfate and heparan sulfate. Does not have beta-N-acetylglucosaminidase activity. Has a high preference for chondroitin-4- sulfate over dermatan sulfate and heparan sulfate. Also hydrolyzes chondroitin-6- sulfate, chondroitin-8- sulfate and chondroitin-9-sulfate. Does not have beta-N-acetylglucosaminidase activity.","Both degrade GAGs, but differ downstream."
"40","CRTA_RUBGE","256","0.2276","no","not-Yes","1","Equally specific","Less informative","no","Involved in the biosynthesis of the carotenoids spheroidene and spirilloxanthin. Catalyzes the introduction of one keto group at the C-2 position of spheroidene and two keto groups at the C-2 and C-2' positions of spirilloxanthin.","Catalyzes the ring opening of trans-3-hydroxy-cis-2-methylcycloprop-1-ene-1-carboxylate (MCP) to produce 3-hydroxy-2-methylpropenyl-1-phosphate (HMP-P).",""
"41","TPX2_PLAF7","216","0.2284","no","Yes","5","Equally specific","More informative","no","Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.","Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.","Same description, but prediction contains additional statement on broader role."
"42","ALF1_TOXGO","363","0.2154","no","not-Yes","2","Equally specific","Less informative","no","Plays a key role in glycolysis by catalyzing the cleavage of fructose 1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Forms a bridge between cell surface adhesins and the actin cytoskeleton. Required for parasite invasion of host cells.","Catalyzes the isomerization of 5-deoxy-alpha-D-xylulose-5- phosphate (DXP) to xylulose 5-phosphate (XylP), the first committed step in xylulose 5- phosphate biosynthesis.","Both manipulate 5-carbon and 6-carbon phosphorylated sugars, but in very different ways."
"43","RT4I1_DROME","413","0.2013","no","Yes","5","Equally specific","More informative","no","NAD(P)H oxidoreductase. Involved in the ubiquinone biosynthetic pathway.","NAD(P)H oxidoreductase involved in the ubiquinone biosynthetic pathway. Required for the O-methyltransferase activity of COQ3. Able to catalyze the oxidoreduction of 3-methoxy-4-hydroxy-6-(all-trans-polyprenylphosphoryl)benzoate (MOBP) to 3-methoxy-4-hydroxy-6-(polyprenylphospho)benzoate (MOBPB) in the ubiquinone biosynthetic pathway.","Same description, but prediction contains additional statement on broader role and pathway."
"44","KAD1_DROME","229","0.2542","no","not-Yes","3","Equally specific","Equally informative","no","Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.","Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo purine and pyrimidine nucleotide biosynthesis.","Both are NMP kinases, Swissprot describes adenine-specific enzyme, while prediction describes pyrimidine-specific."
"45","PAT_SACS2","160","0.1861","no","not-Yes","1","More specific","Less informative","no","Modulates activity of albA1, the major archaeal DNA compaction protein, by decreasing albA1's nucleic acid binding affinity through acetylation of 'Lys-16'.","Catalyzes the hydrolysis of N-(5'-phosphoribosyl)-ATP to form N-(5'-phosphoribosyl)-5'-AMP.",""
"46","AMDA_AGRTU","517","0.2658","no","not-Yes","2","Less specific","Less informative","no","Hydrolase that can catalyze the degradation of ethyl carbamate (also called urethane), a probable human carcinogen widely found in alcoholic beverages. Can also use methyl carbamate, butyl carbamate, acetamide and urea. Also catalyzes the enantioselective hydrolysis of 2-phenylpropionamide, alpha-chlorophenylacetamide, 2- methyl-3-phenylpropionamide and alpha-methoxyphenylacetamide to the corresponding acids. Is inactive on benzamide and L- glutamine.","Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln).",""
"47","BLC8_CITAM","291","0.2501","no","Yes","4","Equally specific","Less informative","no","Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first, third and fourth- generation cephalosporins. Has cefotaxime-hydrolyzing activity. Inactive against cephalosporin antibiotic, cefoxitin, and the carbapenem, imipenem.","Extended-spectrum beta-lactamase (ESBL) which confers resistance to penicillins, as well as first and third generation cephalosporins. Has penicillinase activity.","""Has cefotaxime-hydrolyzing activity."" Swissprot, ""Has penicillinase activity."" prediction. Prediction omits 4th gen activity."
"48","ENDUB_DROME","322","0.2189","no","not-Yes","1","Less specific","Less informative","no","Endoribonuclease that cleaves single-stranded RNAs at uridylates and releases products that have 2'-3'-cyclic phosphate termini. Preferentially cleaves single stranded RNA at poly-U sites with CU, UC and AU sites cleaved less efficiently. May target mRNAs encoding proteins involved in lipid metabolism to regulate their expression. Regulates levels of TBPH protein, but not mRNA, by an as yet unknown mechanism. Important for neuronal development or function.","Endoplasmic reticulum (ER)-bound glycosylphosphatidylinositol-N- acetylglucosaminyltransferase that catalyzes the transfer of an N-acetylglucosamine residue from UDP-N-acetyl-D-glucosamine to a serine or threonine residue and is involved in the remodeling of N-glycans.",""
"49","KDM8_DROME","401","0.2563","no","not-Yes","1","Equally specific","More informative","no","Bifunctional enzyme that acts both as an endopeptidase and 2- oxoglutarate-dependent monooxygenase. May be involved in regulation of behavior and circadian rhythms.","Probable ADP-ribosylase that may play a role in response to DNA damage. May play a role in DNA damage response by mediating ADP-ribosylation of histones H2A and H2AX. ADP-ribosylation of histones is a key step in the DNA damage response by promoting DNA repair and early events of cellular differentiation. Acts as a suppressor of apoptosis.",""
"50","NSH1_CAEEL","338","0.2308","no","not-Yes","4","More specific","Equally informative","yes","Catalyzes the hydrolysis of the N-glycosidic bond of commonly occurring purine and pyrimidine nucleosides into ribose and the base, with decreasing activity, in the order: adenosine, guanosine, inosine, xanthosine, cytidine, uridine.","Catalyzes the hydrolysis of the C-N bond of purine or pyrimidine nucleoside 5'-monophosphates. Is active toward inosine 5'-phosphate (InMP), deoxyinosine 5'-phosphate (dInMP), guanosine 5'-phosphate (GuMP), deoxyguanosine 5'-phosphate (dGuMP), hypoxanthine 5'-phosphate (HxMP), xanthosine 5'-phosphate (XanMP), adenine 5'- phosphate (AdoMP), guanine 5'",""
"51","PMDHL_AERPE","402","0.1349","no","Yes","5","Equally specific","Equally informative","no","Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5- phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).","Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5- phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).","Exact same description."
"52","CGS1_TOBAC","540","0.1988","no","not-Yes","1","Equally specific","Less informative","no","Catalyzes the first committed step of methionine (Met) biosynthesis. Catalyzes the formation of L-cystathionine from homoserine esters and L-cysteine, via a gamma-replacement reaction.","Catalyzes the formation of 5-aminolevulinate from glycine and succinyl-CoA in a two-step reaction.",""
"53","CTMA_CASD6","565","0.1687","no","not-Yes","2","Equally specific","Less informative","no","Involved in the degradation of the cyclic monoterpene limonene. Catalyzes the oxidation of limonene at the primary methyl group, forming perillyl alcohol. Hydroxylates the R- and S-enantiomers to their respective enantiomeric form of perillyl alcohol at a similar rate. Native CtmAB oxidizes a wide range of monocyclic monoterpenes containing the allylic methyl group motif (1-methyl- cyclohex-1-ene). Can also catalyze the reverse reaction, the reduction of perillyl alcohol to limonene, but with lower efficiency. Cannot use molecular oxygen as an electron acceptor. The natural electron acceptor is likely a heterodimeric electron transfer flavoprotein (ETF) (Probable).","Involved in the biosynthesis of the yellow-orange carotenoid staphyloxanthin, which plays a role in the virulence via its protective function against oxidative stress. Catalyzes the oxidation of the double bond between C-7 and C-8 of polyketide carotenoids such as zeaxanthin and antheraxanthin to the corresponding 3,8-didehydro derivatives (3,8-didehydroanthraxanthin and 3,8-didehydrozeaxanthin respectively).","Both are oxidoreductases that act on same subtrate derivatives, but do not agree on actual function."
"54","BGBGP_PAEBO","769","0.2821","no","not-Yes","3","Equally specific","Less informative","yes","Glycoside phosphorylase that catalyzes the reversible phosphorolysis of 3-O-beta-D-glucosyl-D-glucuronate into D-glucuronic acid and alpha-D-glucose 1-phosphate. Cannot phosphorolyze cellobionic acid and laminaribiose. In the reverse direction, using alpha-D-glucose 1-phosphate as a donor substrate, the enzyme acts on D-glucuronate and its artificial derivative p-nitrophenyl-beta-D-glucuronide. The apparent catalytic efficiency towards p-nitrophenyl-beta-D-glucuronide is approximately 5-fold higher than that towards D-glucuronic acid. Is probably involved in the metabolism of oligosaccharides containing the 3-O-beta-D-glucopyranosyl-beta-D- glucuronide structure released from bacterial and plant acidic carbohydrates.","Catalyzes the reversible phosphorolysis of chitobiose (N,N'- diacetylchitobiose or (GlcNAc)(2)) into N-acetyl-alpha-D-glucosamine 1- phosphate (GlcNAc-1-P) and N-acetyl-D-glucosamine (GlcNAc) with inversion of the anomeric configuration. Also catalyzes the reversible phosphorolysis of N,N',N''- triacetylchitotriose ((GlcNAc)(3)) into GlcNAc-1- phosphate (GlcNAc-1-P), GlcNAc-1- phosphate and Glc","Diverge on specific role."
"55","ABF43_HUMIN","481","0.2952","no","not-Yes","2","Equally specific","Equally informative","no","Alpha-L-arabinofuranosidase specific for the cleavage of alpha-1,3-linkage. Shows high activity against 4- nitrophenyl alpha-L-arabinofuranoside, debranched arabinan, and sugar beet arabinan.","Endo-1,5-alpha-L-arabinanase involved in degradation of pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan.","Endo- vs. exo- acting."
"56","EPOXH_BOSS2","288","0.181","no","not-Yes","2","More specific","Equally informative","yes","Catalyzes the hydrolysis of various epoxides into diols. In vitro, shows the strongest activity toward the linear aliphatic epoxide compounds, since it shows strong activity toward (R)-epichlorohydrin, (S)-epichlorohydrin, and 1,2-epoxy-9-decene, but very weak activity toward (R)-styrene oxide, (S)-styrene oxide, and 3,4-epoxy-1- cyclohexene.","Catalyzes the hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Shows highest activity towards 4-chlorobenzoate (4-ClBA) and 4-bromobenzoate (4-BrBA), followed by 4-iodobenzoate (4-IBA), 3-chlorobenzoate (3-ClBA), 3-bromobenzoate (3-BrBA), 2-chlorobenzoate (2-ClBA), 2-brom","Both hydrolases, but different types."
"57","EPOXH_CABSO","329","0.1626","no","not-Yes","2","More specific","Less informative","no","Catalyzes the hydrolysis of various epoxides into diols. In vitro, shows the strongest activity toward aromatic and cyclic aliphatic epoxide compounds, since it shows strong activity toward (R)- styrene oxide, (S)-styrene oxide, and 3,4-epoxy-1-cyclohexene, but very weak activity toward (R)-epichlorohydrin, (S)-epichlorohydrin, and 1,2- epoxy-9-decene.","Involved in the biosynthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Catalyzes the hydrolysis of the C-C bond between C-2 and C-3 of 4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) to yield isopentenyl diphosphate (IPP).","Both hydrolases, but different types."
"58","XHPG_XENHO","266","0.1247","no","not-Yes","2","Equally specific","Equally informative","no","Involved in the biosynthetic pathway of pyrrolizwilline, a pyrrolizidine alkaloid. Catalyzes the N-deacetylation of pyrrolizixenacetamide.","Involved in the biosynthesis of the siderophore enterobactin (enterochelin). Catalyzes the hydrolysis of 5-dehydro-2,3- dihydroxymuconate semialdehyde (DHMS) to yield 2,3-dihydroxymuconate semialdehyde (DHMSA).","Both hydrolases, but different types."
"59","PNAE1_TABIB","263","0.1569","no","not-Yes","2","Less specific","Equally informative","yes","Hydrolase involved in iboga and aspidosperma monoterpenoid indole alkaloids (MIAs, e.g. ibogaine, coronaridine and voacangine) biosynthesis pathway from 19E-geissoschizine, psychoactive compounds likely to be used in the treatment of opioid dependence as anti- addiction agents. Catalyzes the conversion of (-)- voacangine to de-ester-(-)-voacangine which spontaneously converts to (-)-ibogaine on heating. May also convert (-)- coronaridine to de-ester-(-)-coronaridine, but spontaneous conversion to (-)-ibogamine is not observed.","Hydrolase; part of the gene cluster that mediates the biosynthesis of gramillins A and B, bicyclic lipopeptides that induce cell death in maize leaves but not in wheat leaves. The nonribosomal peptide synthetase GRA1 incorporates respectively a glutamic adic (Glu), a leucine (Leu), a serine (Ser), a hydroxyglutamine (HOGln), a 2-amino decanoic acid, and 2 cysteins (CysB and CysA) (Probable). The biosynthesis of 2-amino decanoic acid incorporated in gramillins could be initiated","Prediction incomplete? Both hydrolases, but different types/reactions."
"60","IDI_CANSA","287","0.1115","no","not-Yes","1","Equally specific","Equally informative","no","Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).","Catalyzes the hydrolysis of a carbon-carbon bond at an alpha-position of an acyl group in an acyl-CoA ester, leading to the formation of free fatty acids and acetoacetyl-CoA.",""
"61","HDR_CANSA","464","0.2011","no","not-Yes","1","Equally specific","Less informative","no","Enzyme of the plastid non-mevalonate pathway for isoprenoid biosynthesis that converts 1-hydroxy-2-methyl-2-(E)-butenyl 4- diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is essential for chloroplast development.","N(6)-(pyridoxal phosphate)-L-lysine reductase (PLPR) that catalyzes the NADPH-dependent reduction of N(6)- (pyridoxal phosphate)-L-lysine to form N(6)-(pyridoxal phosphate)-L-lysine mononucleotide, the first step of de novo lysine biosynthesis.",""
"62","ADHB_ASPFN","428","0.1673","no","not-Yes","2","More specific","Less informative","no","Zinc-type alcohol dehydrogenase involved in development, secondary metabolism, pathogenicity, and stress response. Specifically controls the formation of sclerotia and the biosynthesis of aflatoxin. Contribute to seed colonization of A flavus on host maize seed.","Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in a degradation pathway of D-glucose that does not involve intermediate formation of fructose 1,6-bisphosphate.","Both dehydrogenases, but with distinct roles."
"63","GUN5_MICS2","514","0.2514","no","not-Yes","3","Equally specific","Equally informative","no","Endoglucanase that exhibits highest activity toward barley beta-glucan, lower activity toward carboxymethyl cellulose (CMC-Na), and marginal activity toward laminarin and xylan.","Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.","Both glyccoside hydrolases that degrade plant polysaccharides, but differ in substrates (also different sub-categories). Swissprot description mentions activity towards xylan, but 'marginal.'"
"64","CGCS_COLGL","760","0.2366","no","not-Yes","3","Less specific","Equally informative","yes","Bifunctional terpene synthase that converts dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP) into colleterpenol as a single product. The C-terminal prenyltransferase (PT) domain of CgCS catalyzes formation of hexaprenyl diphosphate (HexPP), whereas the N-terminal terpene cyclase (TC) domain catalyzes the cyclization of HexPP to colleterpenol.","Bifunctional terpene synthase; part of the gene cluster that mediates the biosynthesis of the diterpene ent-pimara-8(14),15-diene (PD). Within the cluster, the alpha-amyrin synthase alpha-AS is responsible for the formation of pimara-8(14),15-diene from farnesyl diphosphate (FPP). The next step is the oxidation of pimara-8(14),15-diene by the oxidase PDO to yield pimara-8(14),1","Both bifunctional terpene synthases, but differ downstream."
"65","GH1_SAPOF","449","0.2078","no","not-Yes","1","Less specific","Less informative","yes","Component of the oleanane-type triterpene saponins (e.g. saponarioside A and saponarioside B) biosynthetic pathway, leading to the production of natural products with detergent properties used as traditional sources of soap. Beta-glycosidase that catalyzes the transfer of a modified glucose moiety to QA-triFRXX to produce QA-triF(Q)RXX via the elongation of the C-28 sugar chain with a D-quinovose.","Hydrolyzes p-nitrophenyl-beta-D-glucoside, p-nitrophenyl-beta-D-galactoside, p-nitrophenyl-beta-D-xyloside and p-nitrophenyl-beta-D-mannoside. Does not hydrolyze p-nitrophenyl-beta-D-fucoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-glucuronide, p-nitrophenyl-beta-D-xyloside, p-nitrophenyl-beta-D-galactoside, p-nitrophenyl-beta-D-arabinoside, p-nitrophenyl-beta-D-xyl",""
"66","GT732_SAPOF","490","0.2892","no","not-Yes","3","Equally specific","Less informative","no","Component of the oleanane-type triterpene saponins (e.g. saponarioside A and saponarioside B) biosynthetic pathway, leading to the production of natural products with detergent properties used as traditional sources of soap. A glycosyltransferase that mediates the conversion of QA-triFRX to QA-triFRXX via the elongation of the C-28 sugar chain with a D-xylose.","UDP-glycosyltransferase involved in the biosynthesis of cucurbitacin. Catalyzes the transfer of the 4-O-glucosyl moiety from UDP-glucose to the C-3 hydroxyl group of naringenin chalcone to yield naringenin 4-O- beta-D- glucoside.","Both UDP-hlycosyltransferases in plant metabolism, but describe distinct roles."
"67","BAHD1_SAPOF","459","0.2336","no","not-Yes","2","Equally specific","Less informative","no","Component of the oleanane-type triterpene saponins (e.g. saponarioside A and saponarioside B) biosynthetic pathway, leading to the production of natural products with detergent properties used as traditional sources of soap. An acyltransferase that catalyzes the transfer of an acyl moiety to QA-triF(Q)RXX to produce saponarioside B.","Hydroxycinnamoyl transferase that catalyzes the transfer of an acyl from p-coumaryol-CoA to feruloyl-CoA, caffeoyl-CoA, and sinapoyl-CoA. Can use only p-coumaryol-CoA as acyl acceptor.","Both describe acetyltransferases, but distinct sub-categories and different pathways."
"68","SDR1_SAPOF","302","0.1578","no","not-Yes","2","Less specific","Less informative","no","Component of the oleanane-type triterpene saponins (e.g. saponarioside A and saponarioside B) biosynthetic pathway, leading to the production of natural products with detergent properties used as traditional sources of soap. A dehydrogenase/reductase that, together with UGT74CD1, mediates the conversion of QA-tri to QA-triF; UGT74CD1 may transfer 4-keto-6-deoxy- glucose to QA-tri, which is in turn reduced to D-fucose by SDR1, thus leading to QA-triF formation via the initiation of the C-28 sugar chain.","Short-chain dehydrogenase/reductase involved in lipid metabolism. Catalyzes the reduction of a range of saturated and monounsaturated aldehydes and ketones. Catalyzes the reduction of 4-hydroxynonenal (4-HNE) into 4-hydroxyhexenal. In vitro, catalyzes the reduction of 2,4-dinitrophenylhydrazine (DNPH) to 2,4-dinitrophenylamine (DNP).","Both describe short-chain dehydrogenase/reductase, but differ in specific functions and biological role."
"69","UXNAS_BACCN","321","0.1784","no","not-Yes","1","Equally specific","Less informative","no","Decarboxylase involved in the biosynthesis of the nucleotide- sugar UDP-N-acetylxylosamine (UDP-XylNAc). Catalyzes the NAD-dependent decarboxylation of UDP-N-acetylglucosaminuronic acid (UDP-GlcNAcA) to UDP-XylNAc. Cannot use other UDP- uronates, such as UDP-glucuronic acid (UDP-GlcA) and UDP-galacturonic acid (UDP-GalA).","Catalyzes the conversion of UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UDP-MurNAc-L-Ala-D-Glu) to UDP-N-acetylmuramoyl-L-alanyl-D-alanyl-D-glutamate (UDP-L-alanyl-D-alanyl-D-glutamate).","Although they both describe UDP-linked molecules, they do not share any functional similarities."
"70","PA2A_LACAO","122","0.2256","no","Yes","5","Equally specific","Equally informative","no","PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. In vivo, is non- lethal to mice when intravenously injected up to a concentration of 30 ug, however does show significant edematogenic activity at the injection site.","Snake venom phospholipase A2 (PLA2) that inhibits platelet aggregation. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.","Same description of the function. Only differ in additional information, which is also not conflicting."
"71","OXDA_MAIZE","386","0.2685","no","not-Yes","1","Less specific","More informative","no","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Monooxygenase; part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C. Within the cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin production. The other FUS cluster members are not essential for fusarin C biosynthesis.",""
"72","OXDD_KOMPG","375","0.2004","no","Yes","3","Less specific","Equally informative","no","Selectively catalyzes the oxidative deamination of acidic amino acids. Protects the organism from the toxicity of D-amino acids. Enables the organism to utilize D- amino acids as a source of nutrients. Enables the organism to utilize D-aspartate as a nitrogen source.","Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity. Enables the organism to utilize D-amino acids as a source of nutrients.","Swissprot describes ""selective"" catalysis, prediction describes ""broad substrate specificity"""
"73","GFSG_STRHA","278","0.2027","no","not-Yes","3","More specific","More informative","yes","Methylase required for synthesis of the 16-membered macrolide antibiotics FD-891 and FD-892. In vitro uses S-adenosyl-L-methionine to methylate a number of biosynthetic intermediates in the synthesis of FD-891.","O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the mycotoxin citrinin, a hepato-nephrotoxic compound to humans due to inhibition of respiration complex III. The pathway begins with the synthesis of a keto-aldehyde intermediate by the citrinin PKS (pksCT) from successive condensations of 4 malonyl-CoA units, presumably with a simple acetyl-CoA starter unit. Release of the keto-aldehyde intermediate is consistent with the presence of the C-terminal reductive release domain. pksCT is also able to catalyze the reverse reaction, i.e. catalyze the","Share mechanism and process, but differ in substrate specificty and product."
"74","PAMT_CAPAN","459","0.1402","no","not-Yes","3","Equally specific","Less informative","no","Involved in the biosynthesis of capsaicinoids natural products, pungent alkaloids synthesized from phenylpropanoid intermediates in the placental tissue of chili pepper fruit acting as repellant on herbivorous mammals and conferring spiciness to hot peppers. Can transfer an amine from vanillylamine to pyruvate forming vanillin and L-alanine.","Catalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to use SAM as an amino donor.","Both aminotransferases, share same cofactor, but differ in role."
"75","FDHA2_METMP","676","0.258","no","Yes","5","Equally specific","Less informative","no","Catalyzes the oxidation of formate to carbon dioxide, with coenzyme F420 as the electron acceptor. In vitro can also use methyl viologen as electron acceptor.","Catalyzes the oxidation of formate to carbon dioxide, with coenzyme F420 as the electron acceptor.","Same function description. Swissprot includes additional info on in vitro."
"76","DCI1_PYRO7","280","0.1694","no","not-Yes","1","More specific","More informative","no","Peroxisomal di-isomerase that is involved in fatty acid metabolism enzyme by converting 3,5-dienoyl-CoAs to the corresponding 2,4-dienoyl-CoAs. Involved in fatty acid beta- oxidation, which is important for lipid droplets degradation and infectious growth.","Enoyl-CoA hydratase; part of the gene clusters that mediate the biosynthesis of the host-selective toxins (HSTs) AF-toxins responsible for Alternaria black spot of strawberry disease by the strawberry pathotype. AF-toxin I and III are valine derivatives of 2,3-dyhydroxy-isovaleric acid and 2-hydroxy-isovaleric acid respectively, while AF II is an isoleucine derivative of 2- hydroxy-valeric acid. These derivatives are bound to a 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid",""
"77","CHHY1_CAEEL","458","0.2963","no","not-Yes","3","Equally specific","Less informative","no","Endo-beta-galactosaminidase that specifically hydrolyzes chondroitin, releasing GlcUA-beta-(1->3)-GalNAc-beta-(1->4)-GlcUA-beta- (1->3)-GalNAc as the main product. Also hydrolyzes to a lesser extent chondroitin sulfates (CS-A, CS-C) and hyaluronic acid. May regulate the function of chondroitin in cell division.","Hyaluronidase catalyzes the hydrolysis of hyaluronic acid (HA), an anionic, non-sulfated glycosaminoglycan composed of D-glucuronic acid and N-acetyl-D-glucosamine residues (Probable). Hydrolyzes low molecular weight hyaluronan (HA) into smaller fragments that are not biologically active (Probable).","Both glycoside hydrolases, but differ in roles. While substrates overlap, they are different."
"78","MET2_CRYN9","528","0.1585","no","not-Yes","3","More specific","More informative","yes","Commits homoserine to the methionine biosynthesis pathway by catalyzing its O-acetylation.","N-acetyltransferase; part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties. L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism. The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by the","Both describe O-acetylation of homoserine by FUB3, but prediction describes it within the pathway, rather than the function being annotated."
"79","MAOM_CRYN9","600","0.177","no","not-Yes","3","Equally specific","Less informative","no","NAD-dependent mitochondrial malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate.","This enzyme catalyzes the reversible NADP-dependent decarboxylation of (S)-2- oxoglutarate to succinate and CO(2).","Swissprot describes NAD-dependent, prediction describes NADP-dependent. Both describe decarboxylation within the same context, but mention different reactants."
"80","EXLYS_PBCV1","649","0.2037","no","not-Yes","1","Equally specific","Equally informative","no","During viral entry, involved in the degradation of the host cell wall at the site of attachment.","Promotes colloidosmotic lysis by binding to the midgut epithelial cells of lepidopteran insects.",""
"81","VPAT_PBCV1","197","0.2962","no","not-Yes","2","Equally specific","Less informative","no","Acetylates polyamines such as spermine, spermidine, cadaverine, homospermidine and putrescine (the latter with low efficiency). May play a role in the regulation of polyamine catabolism in the host during viral replication.","Catalyzes the transfer of an acetyl group from acetyl-CoA to the sulfoquinovose component of the sulfolipid biosynthesis pathway.","Both acetyl transfers, differ on functional group."
"82","TRWD_ECOLX","358","0.2941","no","not-Yes","4","Less specific","Less informative","no","Component of the Trw type IV secretion system (T4SS), which is involved in bacterial conjugation of plasmid R388, a conjugative plasmid that confers resistance to sulfonamide and trimethoprim. The TrwD subunit provides energy for pilus biogenesis and substrate transfer. It shows ATPase activity. Also functions together with TrwK/VirB4 to induce a structural change in the pilin, suggesting a role for TrwD as a modulator of TrwK/VirB4 pilin dislocase activity. It may act as a molecular switch between pilus biogenesis and substrate transport. Interacts with the membranous systems to build up the transport assemblage that facilitates DNA transfer.","Component of the VirB/VirD4 type IV secretion system (T4SS), which translocates transfer DNA (T-DNA) and virulence effector proteins into plant host cells, leading to crown gall disease. The VirB11 subunit is essential for virulence. It provides energy for pilus biogenesis and substrate transfer. It binds and hydrolyzes ATP.","Both describe Type IV secretion system (T4SS) VirB11-family ATPase and the same molecular mechanism, but they emphasize different variants of the system."
"83","CLAER_LACPN","217","0.1755","no","not-Yes","1","Equally specific","Less informative","yes","Is involved in a saturation metabolic pathway of polyunsaturated fatty acids, that detoxifies unsaturated fatty acids and generates hydroxy fatty acids, oxo fatty acids, conjugated fatty acids such as conjugated linoleic acids (CLAs), and partially saturated trans-fatty acids as intermediates. CLA-ER catalyzes the saturation of the carbon-carbon double bond in 10-oxo-(11E)-octadecenoate to produce 10-oxooctadecanoate, during linoleate metabolism. As part of the gut microbiome, this enzyme modifies host fatty acid composition and is expected to improve human health by altering lipid metabolism related to the onset of metabolic syndrome.","Involved in the reduction of nitroaromatic compounds. Component of a two-enzyme system that catalyzes the oxidation of nitroaromatic compounds such as 2,4,6-trinitrophenol (2,4,6-TNP) and 2,4-dinitrophenol (2,4-DNP) to the corresponding nitroaromatic acids, i.e. 2,4,6-trinitrotoluene (TNT) and 2,4-dinitrophenol (DNP) to nitroprusside. The nitroreductases AcnB and Ac",""
"84","PRP_SYNY3","133","0.2949","no","not-Yes","3","Equally specific","Equally informative","no","Probably functions in the periplasm as an electron carrier with moderate lateral mobility.","Plays a role in the biogenesis of c-type cytochromes. Functioned probably in heme biosynthesis.","Both describe periplasmic c-type-cytochrome system equally generally, but differ in role."
"85","FDHB_NEOTH","707","0.2521","no","not-Yes","1","Equally specific","Less informative","no","Component of a dehydrogenase that catalyzes the NADP- dependent reduction of CO(2) to formate, the first step in the synthesis of the methyl group of acetate during synthesis of acetate from CO(2). In vitro, can use methyl viologen and benzyl viologen in addition to its natural electron acceptor.","Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CMEP) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP).","Swissprot describes dehydrogenase, but prediction describes lyase."
"86","RHMD_SPHSS","397","0.2119","no","not-Yes","1","Equally specific","More informative","no","Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy- L-rhamnonate (KDR). Also shows activity with L- lyxonate and L-mannonate, with much lower catalytic efficiency. Catalyzes the third step in an alternative pathway for rhamnose utilization that does not involve phosphorylated intermediates.","Isomerase that catalyzes the reversible interconversion of alpha-D-glucose 1-phosphate (Glc-1-P) and beta-D-glucose 1-phosphate (Glc-1-P). Is required for the maintenance of glucose homeostasis. Is also able to act as a D-gluconol-1-phosphate isomerase (D-gluconol-1-P isomerase), but this activity is very low and it is unlikely to play a physiological role in vivo.",""
"87","MCP3_TRYB2","261","0.1605","no","not-Yes","1","Less specific","Equally informative","yes","Promiscuous robust Ala-tRNA deacylase that lacks tRNA discrimination. Deacylates mischarged Ala-tRNA(Pro), Ala-tRNA(Leu) and cognate Ala-tRNA(Ala) with similar efficiencies; more slowly deacylates Ala-tRNA(Thr) and Ala- tRNA(Trp). Uses a size-exclusion based mechanism for deacylation and discriminates against larger amino acids: moderately hydrolyzes Ser-tRNA(Pro) and Gly-tRNA(Pro) and is unable to edit Leu- tRNA(Pro).","Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes",""
"88","KDSR_ASPFU","367","0.2502","no","not-Yes","2","Equally specific","Equally informative","no","Catalyzes the reduction of 3'-oxosphinganine (3- ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.","Catalyzes the reduction of 2-keto-3-deoxy-D-gluconate (KDO) to 2-keto-3-deoxy-D-galactonate (KDG) with NADPH as the electron donor. Can also use NADH as the electron donor.","Both reduction enzymes, but differ in substrates and products."
"89","GLYC_ASPFU","471","0.2383","no","Yes","1","Equally specific","Less informative","no","Catalyzes the interconversion of serine and glycine. Essential for viability and required for virulence in a murine model of established pulmonary infection.","Catalyzes the reversible interconversion of alpha-ketoisovalerate and beta- ketoisovalerate.",""
"90","ENLYS_BP263","486","0.1978","no","not-Yes","2","Equally specific","Less informative","no","Endolysin that degrades host peptidoglycans and participates in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. The N-acetyl- muramidase activity cleaves between N-acetylmuramic acid and N- acetylglucosamine bonds.","Autolysins are involved in the degradation of proteins present in the cell supernatant. Hydrolyzes the bond between the N-terminal amino group of an amino acid and the epsilon-amino group of aspartate or glutamate.","Swissprot describes endolysin, but prediction describes autolysin."
"91","DSD1_DICDI","395","0.2766","no","not-Yes","1","Equally specific","Less informative","no","Catalyzes the conversion of D-serine to pyruvate and ammonia. Plays a role in D-serine detoxification.","Catalyzes the reversible transamination of delta-aminolevulinate (ALA) and glycine to form porphobilinogen (PBG).",""
"92","NDX4_TRYB2","294","0.2435","no","not-Yes","1","Less specific","More informative","yes","Decapping enzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Can also remove GDP from unmethylated capped RNA, but is less active at a mature cap 4 end and thus may function as a surveillance enzyme to decap mRNAs lacking either N7 guanine methylation or ribose 2'O-methylation at nucleosides 2, 3 and 4.","Acts as a negative regulator of the DNA damage response. Involved in the regulation of the cell cycle and the DNA damage response. Plays a role in the regulation of the cell cycle in G2/M phase. Plays a role in the maintenance of genome stability. Plays a role in the regulation of the cell cycle in G2/M phase in response to DNA damage. Acts as a negative regulator of the p53/TP53 pathway in response to DNA damage. Acts as a negative regulator of the p53/TP53 pathway in response to replication stress. Acts as a negative",""
"93","NHMT_EMENI","558","0.2823","no","not-Yes","1","Less specific","More informative","yes","Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation at 'His- 1', which protects the side-chain from oxidative damage. Methylates lytic polysaccharide monooxygenases (LPMOs) destined for secretion, including AN4702.","Alpha-ketoglutarate-dependent dioxygenase; part of the gene cluster that mediates the biosynthesis of the lipopeptide antibiotics leucinostatins that show extensive biological activity, including antimalarial, antiviral, antibacterial, antifungal, and antitumor activities, as well as phytotoxic. Leucinostatin A contains nine amino acid residues, including the unusual amino acid 4-methyl-L-proline (MePro), 2-amino-6-hydroxy-4-methyl-8-oxodecanoic acid (AHyMeOA), 3- hydroxyleucine (HyLeu), alpha-aminoisobutyric acid",""
"94","PR5X_SOLLC","230","0.2944","no","not-Yes","3","Less specific","Less informative","no","Antifungal protein. May bind to beta-glucans and have beta-1,3-D-glucanase activity.","May be involved in protecting the plant body against pathogen attack.","They are both vague, but prediction is vaguer."
"95","ARGV_CORGL","254","0.2642","no","not-Yes","3","Equally specific","Equally informative","no","Part of the ABC transporter complex ArgTUV involved in L- arginine import. May also transport L-citrulline. Probably responsible for energy coupling to the transport system (Probable).","Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system.","Both parts of ABC transporter complex, agree on context, but differ in role."
"96","MIFH1_ONCVO","115","0.1279","no","not-Yes","1","More specific","More informative","yes","Tautomerization of the methyl ester of L-dopachrome. Inhibits migration of human peripheral blood mononuclear cells.","Tartrate-utilizing enzyme; part of the gene clusters that mediate the biosynthesis of AM-toxins, host-selective toxins (HSTs) causing Alternaria blotch on apple, a worldwide distributed disease (Probable). AM-toxins are cyclic depsipeptides containing the 3 residues 2-hydroxy-isovaleric acid (2-HIV), dehydroalanine, L-alanine which are common for all 3 AM-toxins I to III. The fourth precursor is L-alpha-amino-methoxyphenyl-valeric acid (L-Amv) for AM",""
"97","ENG1_HALH5","1020","0.2667","no","Yes","5","Equally specific","Less informative","no","Cleaves internal linkages in 1,3-beta-glucan. May contribute to plant biomass degradation.","Cleaves internal linkages in 1,3-beta-glucan.","Same description of function. Swissprot description includes additional sentence on broader context."
"98","CRLS1_STRCO","215","0.2942","no","not-Yes","1","Equally specific","Less informative","no","Catalyzes the synthesis of cardiolipin (CL) (diphosphatidylglycerol) by specifically transferring a phosphatidyl group from CDP-diacylglycerol to phosphatidylglycerol (PG).","Catalyzes the transfer of phosphatidylglycerol (PG), a major phospholipid in the inner membrane, to the 3'-hydroxyl group of nucleoside triphosphates.","Although they overlap on phosphatidylglycerol (PG), they describe entirely different functions, substrates, and reactions."
"99","FAD1_ARATH","497","0.1966","no","not-Yes","1","Equally specific","Less informative","no","Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Does not exhibit FAD diphosphatase activity but may stably bind FAD non- catalytically.","Catalyzes the hydrolysis of 6-aminopurine riboside 5'-phosphate (6-NPRP) to yield 6- aminopurine 5'-phosphate and ribose 1-phosphate.",""
"100","PDMB_SPHYB","176","0.1982","no","not-Yes","2","Equally specific","Less informative","no","Part of the multicomponent N,N-dimethyl phenylurea N- demethylase responsible for the initial N-demethylation step during the bacterial metabolism of N,N-dimethyl-substituted phenylurea herbicides. Catalyzes the mono-N-demethylation of N,N-dimethyl- substituted phenylurea herbicides to their mono-N-demethylated derivatives. Is active on isoproturon (IPU), chlorotoluron, metoxuron, monoron, diuron, fluometuron and fenuron, but cannot transform the N-methoxy-N-methyl-substituted herbicides.","Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-(1R,2S)-dihydroxy-1,2- dihydronaphthalene. The alpha subunit has a structural role in the holoenzyme.","Both describe parts of multicomponent bacterial oxygenase complexes, but do not agree on substrate, specific function, or role."
"101","AMYG_PHOAM","339","0.2251","no","not-Yes","4","Equally specific","More informative","yes","Geranylgeranyl pyrophosphate synthase; part of the gene cluster that mediates the biosynthesis of a yet unidentified indole diterpene. AmyG catalyzes the first step in indole diterpene biosynthesis by producing geranylgeranyl diphosphate (GGPP).","Geranylgeranyl pyrophosphate synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe geranylgeranyl pyrophosphate synthase, but diff downstream."
"102","SOLAP_PAEBO","768","0.2289","no","not-Yes","2","Less specific","Less informative","no","Catalyzes the reversible phosphorolysis of solabiose. Catalyzes the phosphorolysis and synthesis of solabiose through a sequential bi-bi mechanism involving the formation of a ternary complex. Is probably involved in the metabolism of solabiose released from solabiose-containing compounds.","Catalyzes the hydrolysis of glucose-1-phosphate (G1P) into glucose and phosphate. Is specific for G1P, it is not able to hydrolyze glucose-6-phosphate (G6P) or 2-phospho-D-glycerate (2-PGA).","Both mention glucose phosphate chemistry, but different activites. May actually be contradictory."
"103","PBOC_ASPUT","470","0.2973","no","not-Yes","3","Less specific","Equally informative","yes","O-acetyltransferase; part of the gene cluster that mediates the biosynthesis of protubonine B, a hydroxylated and diacetylated cyclo-L-Trp-L-Leu derivative. Within the pathway, pboC catalyzes the acetylation of protubonine D at the hydroxy group to produce protubonine C. The first step of the protubonine B synthesis is performed by the nonribosomal peptide synthetase pboA that catalyzes the formation of cyclo-L-Trp-L-Leu by condensing L-Leu with L-Trp. The flavin-dependent monooxygenase pboD is responsible for hydroxylation at C-3 of the indole ring and subsequent formation of the pyrrolidine ring, leadind to protubonine D. Protubonine D is further diacetylated by two acetyltransferases, pboB and pboC, to form the final product protubonine B via protubonine C.","O-acetyltransferase; part of the gene cluster that mediates the biosynthesis of pyranonigrins, a family of antioxidative compounds. The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase that condenses 6 malonyl-CoA units to an acetyl starter unit, to form a 1,3,5-trioxotetradecane-6,8-dienyl-ACP. The enoyl reductase (ER) domain of pynA is likely to be functional during the first two rounds of polyketide chain extension, to generate the saturated C-C","Both describe O-acetyltransferase in biosynthesis, but different reactions."
"104","ETPM_ESCWE","403","0.2531","no","not-Yes","3","More specific","Less informative","yes","O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the epipolythiodiketopiperazine melinacidin IV, a major compound produced during pathogenesis. Acromyrmex leafcutter ants form a tripartite superorganismal mutualism with the fungal cultivar Leucoagaricus gongylophorus and a defensive actinobacterial symbiont Pseudonocardia. Melinacidin IV inhibits the ant defensive actinobacterial symbiont Pseudonocardia. The nonribosomal peptide synthetase etpP is speculated to be responsible for condensation of amino acids to form the carbon skeleton of melinacidin IV, whereas the cluster-specific tailoring enzymes are involved in further modifications leading to the production of melinacidin IV (Probable).","O-methyltransferase; part of the gene cluster that mediates the biosynthesis of pyranonigrins, a family of antioxidative compounds. The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase that condenses 6 malonyl-CoA units to an acetyl starter unit, to form a 1,3,5-trioxotetradecane-6,8-dienyl-ACP. The enoyl reductase (ER) domain of pynA is likely to be functional during the first two rounds of polyketide chain extension, to generate the saturated C-C bonds","Both O-methyltransferases, but differ downstream."
"105","ERTE_PAES7","419","0.1428","no","not-Yes","2","Equally specific","More informative","no","Amidohydrolase involved in the utilization of ergothioneine, a naturally occurring histidine analog. Catalyzes the conversion of N-carbamoyl-L-glutamic acid to glutamic acid, ammonia and CO(2), the fifth (last) step of the pathway.","Involved in the catabolism of purine nucleotides. Catalyzes the hydrolysis of all three major types of purine substrates: N(1)-methylpurine, N(1)- methyluracil and N(1)-methyladenosine. It can also use N(1)-acetylpurine, N(1)-acetyluracil and N(1)-acetyladenosine as substrates, with a lower efficiency. It can also use N(1)-formylpurine, N(1)-formyluracil and N(1)-formyladenosine as substrates, with a lower efficiency.",""
"106","ERTD_PAES7","456","0.2578","no","not-Yes","3","Equally specific","Less informative","no","Amidohydrolase involved in the utilization of ergothioneine, a naturally occurring histidine analog. Catalyzes the hydrolysis of hydantoin-5-propionic acid to N-carbamoylglutamate, the fourth step of the pathway.","Catalyzes the conversion of allantoin (5-ureidohydantoin) to allantoic acid by hydrolytic cleavage of the five-member hydantoin ring.","Both describe amidohydrolases, but different reactions."
"107","UAMDH_CAMJU","407","0.2771","no","not-Yes","3","Less specific","More informative","no","Dehydrogenase involved in the biosynthesis of capsular polysaccharides. Catalyzes the NAD(+)-dependent oxidation of UDP-N-acetyl-D-mannosamine (UDP-ManNAc) to UDP-N-acetyl-D- mannosaminuronic acid (UDP-ManNAcA).","Catalyzes the conversion of UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) to UDP-N- acetylmannosamine (UDP-ManNAc), the first step in the biosynthesis of N-acetylglucosamine-6-phosphate (GlcNAc-6-P), the precursor of N-acetylglucosamine, the major constituent of the cell wall.","Both describe the same pathway and function (biosynthesis of surface glycans), but different steps (reactions)."
"108","CDPGS_CAMJU","335","0.1417","no","not-Yes","1","Equally specific","More informative","no","NADP-dependent oxidoreductase involved in the biosynthesis of capsular polysaccharides. Catalyzes the reduction of CDP-6-D-fructose with NADPH to generate CDP-6-D-glucitol.","Catalyzes the irreversible conversion of 3'-deoxy-D-manno-2'- octulosonate (KDO) to phospho-N-acetylglucosamine (PGlcNAc) within the biosynthetic pathway of 2-keto-3-deoxy-D-gluconate (KDG). Catalyzes the reversible conversion of KDO and CTP into CDP-2-keto-3-deoxy-6-phospho-D-gluconate (CDP-KDO).",""
"109","NRP14_CYTMA","573","0.27","no","not-Yes","3","Less specific","Less informative","no","Acyl-CoA ligase; part of the biosynthetic gene cluster 14 that mediates the production of a yet unidentified mycotoxin that contributes to virulence.","Catalyzes the activation of fatty acids by CoA to form acyl-CoA, the first step in fatty acid degradation or biosynthesis.","Swissprot as 'ligase', prediction describes Acyl-CoA ligase action. Swissprot describes specific context."
"110","BDS_ALLAB","363","0.1703","no","not-Yes","1","Equally specific","Less informative","no","Diterpene synthase that catalyzes the conversion of geranylgeranyl diphosphate (GGPP) to bonnadiene. Cannot use geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylfarnesyl diphosphate (GFPP).","Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) to yield the bicyclogermacrene (BGC) precursor germacrene D via a 1,10-cyclization.","Explicitly contradicts Swissprot description."
"111","PMS_ALLAB","339","0.1987","no","not-Yes","1","Equally specific","Less informative","no","Diterpene synthase that catalyzes the conversion of geranylgeranyl diphosphate (GGPP) to phomopsene, a diterpene previously reported from the fungus P.amygdali. Phomopsene is the main product, but the enzyme can also produce allokutznerene (about 50% of phomopsene production activity) and traces of spiroviolene. Cannot use geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylfarnesyl diphosphate (GFPP).","Terpene cyclase that catalyzes the cyclization of (2E,6E)- farnesyl diphosphate (FPP) to produce the sesquiterpene beta-caryophyllene. Is also able to use (2E)-geranyl diphosphate (GPP) as substrate for sesquiterpene synthesis, but not geranylgeranyl diphosphate (GGPP).","Explicitly contradicts Swissprot description."
"112","THIOL_METTL","392","0.1632","no","not-Yes","3","Equally specific","Equally informative","no","Catalyzes the condensation of two acetyl-coA molecules into acetoacetyl-CoA. Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids.","Component of the BbsAB thiolase complex, which catalyzes the thiolytic cleavage of (S)-2-butenoyl-CoA to form (2R,3S)-2,3-dihydroxybutanoyl-CoA, the intermediate in fatty acid synthesis. The BbsA subunit catalyzes the transfer of the thioester moiety from CoA to the carrier protein (CP) domain of BbsB to form the covalent thioester bond.","Differ in specific role."
"113","LP9B_BOTFB","322","0.2984","no","Yes","5","Equally specific","Equally informative","no","Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 and C4 oxidation products (Probable). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (Probable).","Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.","Exact same description."
"114","LAD_AURME","368","0.2203","no","not-Yes","1","More specific","More informative","no","Plays a key role in liamocins biosynthesis by providing the arabinol moity that is linked to 3,5-dihydroxydecanoic acid (provided by the HR-PKS PKS1) via ester bond formation catalyzed by the esterase EST1.","Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose via a non-phosphorylative variant of the Entner-Doudoroff pathway.",""
"115","CHOD_ACIBA","556","0.1795","no","not-Yes","2","More specific","Equally informative","no","Bifunctional enzyme that catalyzes the oxidation and isomerization of cholesterol to cholestenone (cholest-4-en-3-one), an initial step in the cholesterol degradation process.","FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C. Within the cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin production. The other FUS cluster members are not essential for fusarin C biosynthesis.","Both FAD-dependent monooxygenase, but differ downstream."
"116","PENIA_PENPA","372","0.2307","no","not-Yes","3","Less specific","Less informative","no","Sesquiterpene cyclase; part of the gene cluster that mediates the biosynthesis of penifulvin A, a potent insecticidal sesquiterpene that features a [5.5.5.6]dioxafenestrane ring. Within the pathway, peniA catalyzes the first step and generates the angular triquinane scaffold silphinene via cyclization of the linear farnesyl pyrophosphate (FPP). The cytochrome P450 monooxygenase peniB and the flavin-dependent monooxygenase peniC then catalyze a series of oxidation reactions to transform silphinene into penifulvin A.","Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) to yield the bicyclic sesquiterpene (+)-gamma-cadinene via a 1,10-cyclization, which requires the abstraction of the pyrophosphate from FPP to yield a (+)-germacradienyl cation. The only accepted substrate is farnesyl diphosphate (FPP).","Both describe sesquiterpene cyclase and FPP as a substrate, but they describe different cyclization mechanisms and products."
"117","AB62C_PENPR","328","0.2146","no","not-Yes","3","Equally specific","Less informative","no","Alpha-L-arabinofuranosidase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Debranches beta-cellulose by removing 1,3 monosubstituted arabinose.","Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.","Both describe action on xylan and the same context, but describe different specific enzymes that act on different bonds."
"118","CUBB_PSICU","344","0.1946","no","not-Yes","4","Equally specific","Less informative","yes","Terpene cyclase that catalyzes the cyclization of (2E,6E)- farnesyl diphosphate (FPP) to produce the sesquiterpene (3R,6E)- nerolidol. Is also able to use (2E)-geranyl diphosphate (GPP) as substrate to produce the monoterpene (R)-linalool.","Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to various sesquiterpenes, including alpha-copaene, beta-copaene, beta-caryophyllene, gamma-cadinene, delta-cadinene, delta-3-carene, germacrene D, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol, alpha-cadinol,","Both terpene cyclases that produce sesquiterpenes through the same mechanism, but differ in product description."
"119","AB62A_PENPR","384","0.2009","no","not-Yes","2","Less specific","Less informative","no","Alpha-L-arabinofuranosidase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Releases L-arabinose from arabinoxylan.","Involved in the degradation of plant cell wall polysaccharides. Hydrolyzes (1->4)-beta-D-glucan, (1->3)-beta-D-glucan and (1->6)-beta-D-glucan.","Both describe glycoside hydrolase involved in the breakdown of structural plant cell wall polysaccharides, but do not agree on the actual function or targets (pentose/arabinose vs. hexose/glucose)."
"120","AKS2_ALSSC","450","0.2486","no","not-Yes","3","Equally specific","Equally informative","no","Acyltransferase involved in the biosynthesis of akuammilan monoterpene indole alkaloids (MIAs) natural products, components with various biological properties such as antidiabetic, antibacterial, anti-inflammatory, anticancer, and antimalarial activities. Catalyzes the conversion of rhazimol to akuammiline.","Acetyltransferase involved in the biosynthesis of limonoids triterpene natural products such as azadirachtin, an antifeedant widely used as bioinsecticide, and possessing many medicinal applications including anti-cancer, anti-malarial, anti-rheumatic, antibacterial, anti-inflammatory, anti-pyretic and diuretic effects. Catalyzes the formation of 7-O-acetyl-7-O-acetylnimbinin from 7-O-acetylnimbinin and acetyl-CoA.","Both perform the same function (transferring an acetyl/acyl moiety), but differ in specific role."
"121","APHA_CRYP1","504","0.2021","no","not-Yes","3","More specific","More informative","yes","Acid phosphatase involved in the regulation of fungal phenotypic traits and virulence in C.parasitica.","Phosphatase that catalyzes the hydrolysis of phosphoanhydride bonds of a variety of phosphoanhydrides, including phosphoenolpyruvate (PEP), beta-glycerophosphate (beta-GP), adenosine 5'- phosphosulfate (APS), and inorganic pyrophosphate (PPi). Has no activity toward phosphoethanolamine (PE), phospho-L-serine (PS), phospho-L-aspartate (PA), phospho-L-threonine (PT), and phospho-L-histidine (PH). May be involved in regulating the intracellular levels of","Both phosphatases, differ in role and context."
"122","CHIT2_DREDA","773","0.2372","no","Yes","4","Equally specific","Equally informative","no","GPI-anchored chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Required to reshape the cell wall at the sites where cell wall remodeling and/or cell wall maturation actively take place such as sites of conidia formation. Involved in trapping nematodes.","GPI-anchored chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Required to reshape the cell wall at the sites where cell fusion occurs during mating.","Same function description, but mention different biological contexts."
"123","GCDH_DREDA","385","0.2277","no","not-Yes","2","Equally specific","Less informative","no","Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L- hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Involved in trapping nematodes.","Involved in the degradation of short-chain fatty acids (SCFA) such as acetate, propionate and butyrate. Catalyzes the reversible conversion of 2-methylpropanoate (isobutanoate) to 4-methyl-2-oxopentanoate. Is also able to catalyze the reversible conversion of 2-methylbutanoate (isovalerate) to 4-methyl-2-oxopentanoate and 2-methylbutanoyl-CoA to 4-methyl-2-oxopentanoyl-CoA.","Both act on specialized carboxylic acid derivatives, but differ in mechanism, role, and context."
"124","CHIT1_DREDA","949","0.2432","no","Yes","4","Equally specific","Equally informative","no","Chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Involved in trapping nematodes.","Secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Participates in the infection process and plays a role in cell separation.","Very similar description, but disagree on broader biological context only."
"125","MANC_DREDA","400","0.2788","no","not-Yes","2","Equally specific","Equally informative","no","Probable endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Involved in trapping nematodes.","Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.","Both describe endo-acting glycoside hydrolase, but different substrates."
"126","HODMB_ECTM1","309","0.2158","no","not-Yes","2","Equally specific","Less informative","yes","Component of the heme-dependent oxidative N-demethylase (HODM) enzyme, that catalyzes the NADPH-dependent oxidation of dimethylamine (DMA) to methylamine (MA) and formaldehyde. Functions in bacterial methylated amine catabolism, linking alkylamine oxidation to the tetrahydrofolate C1 pool. The beta subunit of HODM binds FMN and a 2Fe-2S cluster, and likely reduces the ferric heme iron of the alpha subunit to ferrous using NADPH.","Involved in the degradation of 2,4-dichlorophenol (2,4-DCP). Catalyzes the reduction of 2,4-DCP to 2,4-dihydro-2,4-dichlorobenzoate (2,4-DH-2,4-DCP). It can also reduce 2,4,6-trichlorophenol (2,4,6-DCP) to 2,4,6-dihydro-2,4,6-trichlorobenzoate (2,4,6-DH-2,4,6","Both describe the same general function (bacterial catabolic oxidoreductase/reductase components) but describe different chemical reactions, pathways, and target substrates."
"127","PLYA_SCLS1","329","0.2905","no","Yes","5","Equally specific","Equally informative","no","Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins. Favors pectate, the anion, over pectin, the methyl ester.","Pectinolytic enzyme consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Among pectinolytic enzymes, pectin lyase is the most important in depolymerization of pectin, since it cleaves internal glycosidic bonds of highly methylated pectins. Favors pectate, the anion, over pectin, the methyl ester.","Exact same description."
"128","FDRB_SORC5","244","0.2915","no","not-Yes","3","Equally specific","Less informative","no","Transports electrons between NADPH and ferredoxin. Can transfer electrons to ferredoxins Fdx2 and Fdx8. Prefers NADPH to NADH.","Transports electrons between flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD).","Both describe oxidoreductases in flavoprotein electron transfer modules, but differ in roles and substrates."
"129","HASG_ASPFC","468","0.1705","no","not-Yes","3","Equally specific","Less informative","yes","FAD-linked oxidoreductase; part of the gene cluster that mediates the biosynthesis of hexadehydro-astechrome (HAS), a tryptophan-derived iron(III)-complex that acts as a virulence factor in infected mice. Within the pathway, hasG converts the prenyl to a methylbutadienyl side chain. The HAS biosynthesis begins with the synthesis of a tethered Trp-Ala dipeptide by the NRPS hasD. The 7-dimethylallyltryptophan synthase hasE then catalyzes the prenylation of the hasD-tethered tryptophan or the resulting tethered Trp-Ala dipeptide at the C-7 position of the indole moiety. HAS biosynthesis continues via tethered intermediates with the succesive actions of the cytochrome P450 monooxygenase hasH, the O- methyltransferase hasC, and the FAD-linked oxidoreductase hasG. The resulting O-methylated diketopiperazine is then released from hasD. Finally, three O-methylated diketopiperazine molecules assemble in a trimeric complex with Fe(III) to produce hexadehydro-astechrome.","FAD-linked oxidoreductase; part of the gene cluster that mediates the biosynthesis of squalestatin S1 (SQS1, also known as zaragozic acid A), a heavily oxidized fungal polyketide that offers potent cholesterol lowering activity by targeting squalene synthase (SS). SQS1 is composed of a 2,8- dioxobicyclic[3.2.1]octane-3,4,5-tricarboxyclic acid core that is connected to two lipophilic polyketide arms. These initial steps feature the priming of an unusual benzoic acid starter unit onto","Both FAD-linked oxidoreductases in BGC, but differ downstream."
"130","LP9C_PODAN","287","0.2244","no","Yes","5","Equally specific","Equally informative","no","Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 or C4 oxidation products (Probable). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (Probable).","Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C1 or C4 oxidation products. Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate.","Exact same description."
"131","KSL1_RICCO","738","0.2015","no","not-Yes","4","More specific","More informative","no","Diterpene cyclase involved in the biosynthesis of labdane- related diterpenoids (LRDs) natural products. Catalyzes the cyclization of ent-CDP into ent-kaurene.","Involved in the biosynthesis of ent-kaurene diterpenoids natural products, such as miltiradiene, a major component of the essential oil of mint and other aromatic plants, and potential bioactive compounds. Catalyzes the conversion of ent-copalyl diphosphate (ent-CPP) and ent-kaurene diphosphate (ent-KPP) into ent-kaurene (ent-K) and ent-kaurenoic acid, respectively.","Agree on reaction, pathway, but prediction is more specific."
"132","MRSB_PSESY","387","0.1555","no","not-Yes","1","Less specific","Equally informative","no","Aminotransferase involved in the formation of the rare amino acid 3-methylarginine (MeArg), which is used as a potent antibiotic against the closely related soybean pathogen P.syringae pv. glycinea. Probably catalyzes transamination from the donor L- aspartate to 5-guanidino-3-methyl-2-oxopentanoic acid, generating 3- methylarginine (Probable).","Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3- dihydroxybenzoyl)-serine. Enterobactin is capable of transporting iron from the environment into the cell, and thus plays an important role in maintaining iron homeostasis.",""
"133","HPS_STRCL","321","0.2012","no","not-Yes","3","Equally specific","Equally informative","no","Terpene synthase that catalyzes the conversion of geranylgeranyl diphosphate (GGPP) into a mixture of diterpenes, including hydropyrene (HP), hydropyrenol (HPol), isoelisabethatriene and traces of isoelisabethatriene B. Hydropyrene is the main product. Some other diterpenoids are also produced in very low quantities.","Catalyzes the conversion of (2E,6E)-farnesyl diphosphate (FPP) to yield the bicyclic sesquiterpene (1R,6S,7S)-(-)-(E)-beta-caryophyllene via a 1,10-cyclization, which requires the abstraction of the pyrophosphate from FPP to yield a (E)-beta-caryophyllene cation.","Both describe terpene cyclase in the same context, but differ in specific role."
"134","FGL2_GIBZE","408","0.2991","no","Yes","4","Equally specific","Equally informative","yes","Secreted mono- and diacylglycerol lipase involved in plant virulence. Has a substrate preference for p- nitrophenyl esters with a carbon chain length of C10 (p-nitrophenyl caprate).","Secreted lipase that catalyzes the hydrolysis of triacylglycerols to free fatty acids and mono- and diacylglycerols as well as the hydrolysis of phosphatidylethanolamines to phosphatidic acid. Is able to hydrolyze the synthetic substrate p-nitrophenyl palmitate (PNP-C16:0) as well as the natural substrates triacylglycerol 1,3-dioleoylglycerol (1,3-DOG), triacylglycerol 1,3-dioleoylglycerol (1,3-DOG) and triacylglycerol 1,2-dioleoylglycerol (1,2-","Both describe ligase degrading glycerol lipids, but disagree on the sub-type of the substrate (mono/diacyl vs. triacyl/phospholipid)."
"135","FGL4_GIBZE","556","0.2052","no","Yes","3","Equally specific","Equally informative","yes","Secreted lipase involved in plant virulence. Has a substrate preference for p-nitrophenyl esters with a carbon chain length of C12 (p-nitrophenyl laureate).","Secreted lipase that catalyzes the hydrolysis of triacylglycerols into free fatty acids and glycerol, and of diacylglycerols into free fatty acids and monoacylglycerols. Can also hydrolyze p-nitrophenyl palmitate (p-NPP) and 4- methylumbelliferyl palmitate (4-MUP), but not 4-methylumbelliferyl oleate, 4-methylumbelliferyl stearate, 4-methylumbelliferyl laurate, 4-methylumbelliferyl myristate, 4-methylumbelliferyl palmitoleate,","Both describe secreted lipase hydrolyzing acylglycerol substrates, but Swissprot description specifically mentions preference for C12 chains, and prediction avoids C12 chains and prefers C16 chains."
"136","FGL5_GIBZE","535","0.2085","no","Yes","4","Equally specific","Equally informative","yes","Secreted lipase involved in plant virulence. Has a substrate preference for p-nitrophenyl esters with a carbon chain length of C8 (p-nitrophenyl caprylate).","Secreted lipase that is able to hydrolyze triacylglycerols into free fatty acids and glycerol. Is also able to hydrolyze 4-methylumbelliferyl acetate, 4- methylumbelliferyl butyrate, 4-methylumbelliferyl hexanoate, 4-methylumbelliferyl heptanoate, 4-methylumbelliferyl laurate, 4-methylumbelliferyl myristate, 4-methylumbelliferyl palmitate, 4-methylumbelliferyl stearate, 4-methylumbelliferyl oleate and 4-","Both describe secreted lipase hydrolyzing glycerol-based lipids and processing synthetic ester substrates. Prediction description mentions more diverse targets, and Swissprot mentions different specificity."
"137","SIDF_BEAB2","455","0.2989","no","not-Yes","2","Equally specific","Equally informative","yes","Hydroxyornithine transacylase; part of the gene cluster that mediates the biosynthesis of at least 11 siderophores, including beauverichelin A, dimerumic acid (DA), Na-dimethyl coprogen (NADC), eleutherazine B, ferricrocin (FC), fusarinine A, fusarinine C (FsC), metachelin A, mevalonolactone, rhodotorulic acid (RA) and tenellin. This cocktail of siderophores for iron metabolism is essential for virulence, and more specifically for the fungal virulence in penetrating through the host cuticle. Siderophore synthesis is also involved in conidial germination under iron-deficient conditions. For biosynthesis of fusarinine C, the transacylase SIDF transfers anhydromevalonyl to N(5)-hydroxyornithine. The required anhydromevalonyl-CoA moiety is derived from mevalonate by CoA ligation and dehydration catalyzed by SIDI and sidH respectively.","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of fumitremorgins, indole alkaloids that carry not only intriguing chemical structures, but also interesting biological and pharmacological activities. The biosynthesis of fumitremorgin-type alkaloids begins by condensation of the two amino acids L-tryptophan and L-proline to brevianamide F, catalyzed by the non-ribosomal peptide synthetase ftmA. Brevianamide F is then prenylated by the prenyltransferase ftmPT1/ftmB in the presence of dimethylallyl diphosphate, resulting in the formation of tryprostatin B. The","Both mention action on amino acids in fungal biosynthetic pathways, but perform different functions."
"138","KHSE_CRYN9","357","0.2288","no","not-Yes","3","Equally specific","Equally informative","no","Commits homoserine to the threonine biosynthesis pathway by catalyzing its O-phosphorylation.","Catalyzes the phosphorylation of N-acetyl-beta-alanine to N-acetyl-beta-alanine phosphate.","Both describe amino acid kinases, but different substrates and pathways."
"139","ARGM_STREL","369","0.1505","no","not-Yes","1","Less specific","Less informative","yes","Aminotransferase involved in the formation of the rare amino acid 3-methylarginine (MeArg), which is incorporated into the peptidyl nucleoside antibiotic arginomycin. Catalyzes two rounds of transamination: the transfer of the amino group from L- arginine to 2-oxoglutarate to give glutamate and 5-guanidino-2- oxopentanoic acid, which will be methylated by ArgN. Then, ArgM specifically catalyzes transamination from the donor L- aspartate to the 5-guanidino-3-methyl-2-oxopentanoic acid produced by ArgN, generating the final product, 3-methylarginine. Cannot use arginine analogs, such as D-arginine, L-homoarginine and N- methylarginine for the first transamination.","Involved in the biosynthesis of 2-amino-4-methoxy-6-nitro-7,8-dihydro-8-oxononanoate (ANM), a substrate required for the synthesis of the bacterial coenzyme A. Catalyzes the conversion of 4-carboxy-2-hydroxymuconate-6-semialdehyde (CHMS) to 2-amino-4-methoxy-6-nitro-7,8-dihydro-8-oxononanoate (ANM) and of 4-carboxy-2- hydroxymuconate-6-phosphate (",""
"140","PHNY_GIMM8","260","0.2418","no","not-Yes","1","Equally specific","Less informative","no","Part of an oxidative pathway for utilization of methylphosphonic acid as a phosphate source. Catalyzes the conversion of methylphosphonic acid to hydroxymethylphosphonic acid. Is specific for the hydroxylation of methylphosphonate.","Involved in the biosynthesis of the osmoprotectant glycine betaine (GB). Catalyzes the epimerization of L-carnitine, which is an intermediate in the biosynthesis of GB.",""
"141","3BHD_KLEAE","337","0.1823","no","not-Yes","1","Equally specific","Equally informative","no","Catalyzes the degradation of estradiol into estrone. Can also degrade testosterone.","Involved in the biosynthesis of the osmoprotectant glycine betaine, which is active as a stress protectant. Catalyzes the second step in the biosynthesis of glycine betaine, the NAD(P)H-dependent reductive activation of choline.",""
"142","CLADC_LACPN","281","0.1573","no","not-Yes","2","Equally specific","Equally informative","yes","Is involved in a saturation metabolic pathway of polyunsaturated fatty acids, that detoxifies unsaturated fatty acids and generates hydroxy fatty acids, oxo fatty acids, conjugated fatty acids such as conjugated linoleic acids (CLAs), and partially saturated trans-fatty acids as intermediates. CLA-DC catalyzes the migration of the carbon-carbon double bond in 10-oxo-(12Z)-octadecenoate to produce 10-oxo-(11E)-octadecenoate, during linoleate metabolism. As part of the gut microbiome, this enzyme modifies host fatty acid composition and is expected to improve human health by altering lipid metabolism related to the onset of metabolic syndrome.","Isomerase; part of the gene cluster that mediates the biosynthesis of squalestatin S1 (SQS1, also known as zaragozic acid A), a heavily oxidized fungal polyketide that offers potent cholesterol lowering activity by targeting squalene synthase (SS). SQS1 is composed of a 2,8- dioxobicyclic[3.2.1]octane-3,4,5-tricarboxyclic acid core that is connected to two lipophilic polyketide arms. These initial steps feature the priming of an unusual benzoic acid starter unit onto the highly reducing","Both describe isomerase, but different substrates, pathways, roles, and contexts."
"143","STSC_STRE0","299","0.272","no","not-Yes","2","Less specific","More informative","yes","Sesterterpene cyclase, which converts geranylfarnesyl diphosphate (GFPP) into the terpenes somaliensene A and somaliensene B.","Bifunctional polyketide synthase; part of the core atranone cluster (CAC) which products are predicted to catalyze most or all steps of mycotoxin atranone synthesis, starting from geranylgeranyl pyrophosphate (GGPP). The initial cyclization of GGPP to dolabellane is probably performed by the terpene cyclase ATR13. The Baeyer-Villiger oxidation near the end of the atranone synthesis, which converts atranones D and E to atranones F and G is predicted to be catalyzed by the monooxygenase ATR8. Of the CAC's other predicted gene","Both describe isoprenoid cyclization, but differ in substrates, pathways, and roles."
"144","CHCC2_SINCY","590","0.1819","no","not-Yes","2","Equally specific","Equally informative","no","Desaturase involved in a cyclohexanecarboxylate (CHCA) degradation pathway. Probably catalyzes the conversion of 4-oxocyclohexenecarboxylate to 4- oxocyclohex-2,5-dienecarboxylate, which is spontaneously isomerized to 4-hydroxybenzoate (4-HBA).","Involved in the degradation of 3,5-dihydroxybenzoate (3,5-DHB). Catalyzes the oxidation of 3,5-DHB to 3,6-dihydroxybenzoate (3,6-DHB). Also catalyzes the isomerization of 3,5-DHB to 2,4-dihydroxybenzoate (2,4-DHB) and 3,6-DHB to 3,7-dihydroxybenzoate (3,7-DHB).","Both are oxidoreductases that target carboxylated structures in degradation, but differ in catalytic mechanisms."
"145","PNBA_NOCS0","247","0.2224","no","Yes","3","Equally specific","More informative","yes","Nitroreductase involved in the degradation of nitroaromatic compounds. Catalyzes the conversion of 4- nitrobenzoate to 4-hydroxylaminobenzoate.","Catalyzes the NAD(P)H-dependent reduction of nitroaromatic compounds such as 2,4-dinitrotoluene (DNT), 2,4,6-trinitrotoluene (TNT) and 1,3,5-trinitro-1,3,5-triazine (RDX). In addition, it can also reduce other nitroaromatics such as 2,4-dinitrochlorobenzene (DNCB), 2,4,6-trinitrophenol (TNP), 2,4-","Both are nitroreductases that act on similar compounds, but differ in substrate specificity."
"146","SDR_MELAZ","271","0.1502","no","not-Yes","1","Less specific","Less informative","no","Oxidoreductase involved in the biosynthesis of limonoids triterpene natural products such as azadirachtin, an antifeedant widely used as bioinsecticide, and possessing many medicinal applications including anti-tumoral, anti-malarial, anti-rheumatic, antibacterial, anti-inflammatory, anti-pyretic and diuretic effects. Catalyzes the oxidation of 21-O-acetyl-isomeliandiol to (21S)-21- acetoxyl-apo-melianone.","Involved in the biosynthesis of gibberellins (GAs), diterpenes that play an important role in plant development. Catalyzes the conversion of ent-copalyl diphosphate (ent-CPP) to ent-kaurene diphosphate (ent-KPP).",""
"147","OYE_CABCO","674","0.1253","no","not-Yes","2","More specific","Equally informative","yes","A member of the 2-enoate reductase subfamily of old yellow enzymes (OYE) able to reduce alpha/beta alkenes near electron- withdrawing groups as well as perform oxidative demethylation chemistry. Prefers NADH over NADPH as cosubstrate. May play a role in osmotic stress response in situ (Probable).","NADH-dependent oxidoreductase with a broad substrate specificity. Catalyzes the reduction of 3-hydroxyacyl-CoA and (R)-2-hydroxyacyl-CoA to 3-oxoacyl-CoA and (R)-2-hydroxyacyl-CoA, respectively. Can catalyze the reduction of 3-ketoacyl- CoA to 3-oxoacyl-CoA, but not the reverse reaction. Also catalyzes the reduction of 2-ketoacyl-CoA to 2-hydroxyacyl-CoA, but not the reverse reaction. Shows low activity with (S)-2-hydroxyacyl","Both are NADH-dependent oxidoreductases, but function differently (different bonds)."
"148","PLA2_CALPY","162","0.2858","no","Yes","5","Equally specific","Equally informative","no","PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.","PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.","Exact same description."
"149","ABPB_ASPTN","379","0.2862","no","not-Yes","1","Equally specific","Equally informative","no","Trans-prenyltransferase that acts in both the aspulvinones and butyrolactones pathways. Prenylates aspulvinone E and butyrolactone II to yield repectively aspulvinone H and butyrolactone I.","Involved in the alkaloid biosynthesis. Catalyzes the 10-hydroxylation of the indole moiety of tryptamine to form 5-hydroxytryptamine.",""
"150","AOIO_ASPOR","398","0.2235","no","Yes","3","Equally specific","More informative","yes","O-methyltransferase; part of the gene cluster that mediates the biosynthesis of a methylated derivative of known natural products orthosporin and diaporthin. Does not seem to be involved in the biosynthesis of the identified final product of the pathway and its function has still to be determined.","O-methyltransferase; part of the gene cluster that mediates the biosynthesis of azaphilones, a class of fungal metabolites characterized by a highly oxygenated pyrano-quinone bicyclic core and exhibiting a broad range of bioactivities. In the first step, the non-reducing polyketide synthase azaA forms the hexaketide precursor from successive condensations of five malonyl-CoA units, presumably with a simple acetyl-CoA starter unit. The reactive polyketide chain then undergoes a PT- mediated C2-C7 cyclization to afford the aromatic ring and is eventually released as an aldehyde through the R-domain. The","Both are O-methyltransferases, but synthesize different products."
"151","PYLD_METBF","263","0.2364","no","not-Yes","1","Equally specific","Equally informative","yes","Catalyzes the ultimate step of the pyrrolysine biosynthesis pathway by converting the isopeptide (3R)-3-methyl-D-ornithyl-N(6)-L- lysine to the 22nd proteinogenic amino acid. Is able to use surrogate substrates such as (3R)-D-ornithyl-N(6)-L-lysine in vitro.","Bifunctional enzyme that catalyzes two of the steps in the biosynthesis of the branched-chain amino acids (BCAA) leucine, isoleucine and valine. Binds pyridoxal 5'-phosphate (PLP) but does not catalyze its condensation with acetoacetyl-CoA to form 2-acetolactate. Catalyzes the alpha-amination of 2-acetolactate to form (2S,3S)-2,3-dihydroxy-3-methylbutanoate ((2S,3S)-2,3-dihydroxy-3-methylbutanoyl-",""
"152","OLSC_RHITR","281","0.2131","no","not-Yes","1","Equally specific","Less informative","no","Involved in the biosynthesis of ornithine lipids (OLs), which are phosphorus-free membrane lipids. Catalyzes the hydroxylation at the 2 position of the secondary fatty acid of OL. Contributes to symbiotic performance and acid tolerance.","Catalyzes the hydrolysis of long-chain fatty acyl-coenzyme A (acyl-CoA) to yield a fatty acid and an acyl-CoA. This is the first step in the degradation of sphingolipids such as ceramide which contain a long-chain fatty acid chain.","Swissprot describes biosynthesis, but prediction describes hydrolysis. Explicit contradiction."
"153","AURI_STRTU","230","0.2668","no","not-Yes","3","Equally specific","More informative","no","Methyltransferase involved in the biosynthesis of the antibiotic aureothin, a nitroaryl polyketide metabolite with antifungal, cytotoxic and insecticidal activities. Catalyzes the methylation of demethylluteothin to luteothin (also called deoxyaureothin). Is specific for its gamma- pyrone substrate, and does not act on the alpha-pyrone isomer.","O-methyltransferase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The first step of the pathway is the synthesis of 6-methylsalicylic acid methyl ester (6-MSAME) by the polyketide synthase aumA. 6-MSAME is then converted to protoarthripenoid A by the cytochrome P450 monooxygenase aumB. Protoarthripenoid A is further oxidized to arthripenoid A by the cytochrome P450 monooxygenase aumC. The FAD-dependent monooxygenase aumD","Both are methyltransferases with same substrate class, but differ in role. and pathway."
"154","SMOA_AGRFC","174","0.1901","no","not-Yes","4","Equally specific","Less informative","no","Part of the sulfoquinovose monooxygenase (sulfo-SMO) pathway, a D-sulfoquinovose degradation pathway that enables the complete utilization of all carbons within sulfoquinovose (SQ) with concomitant production of inorganic sulfite. Catalyzes the NADH- dependent reduction of FMN. FMNH(2) is then transferred to the sulfoquinovose monooxygenase SmoC.","Catalyzes the reduction of FMN to FMNH2 which is then used to reduce pyrimidine N(1)- mononucleotide (mN(1)N) to give pyrimidine N(1)- nucleoside. Can also reduce pyrimidine N(3)- mononucleotide (mN(3)N) to pyrimidine N(3)-nucleotide (N(3)N).","Only differ in downstream (secondary) fate of the product."
"155","BGLR_STRA5","599","0.2347","no","not-Yes","1","Equally specific","Less informative","yes","Displays beta-glucuronidase activity with the artificial substrate p-nitrophenyl-beta-D-glucuronide (PNPG). Is likely capable of scavenging glucuronate from a range of chemically distinct xenobiotic and endobiotic glucuronides present in the gastrointestinal (GI) tract, to be able to utilize these diverse sources of carbon. As part of the GI microbiome, this enzyme would be able to reactivate glucuronide drug conjugates, such reactivated compounds can significantly damage the GI tract.","Component of a beta-galactosidase. Acts on galacto-beta-(1->3)-galacto-beta-(1->6)-galacto-beta-(1->4)-D-galactose (lacto-triose), galacto-beta-(1->4)-galacto-beta-(1->6)-galacto-beta-(1->4)-D-galactose (lacto-tetraose) and galacto-beta-(1->6)-galacto-beta-(1",""
"156","WBGN_ECOLX","290","0.2035","no","not-Yes","1","Equally specific","Less informative","no","Involved in the biosynthesis of the lipopolysaccharide (LPS) O-antigen region. Catalyzes the transfer of colitose from GDP-colitose to the galactose residue of beta-Gal-(1->3)-GlcNAc (lacto-N-biose) via an alpha1,2-linkage. Is specific for beta-Gal-(1->3)-GlcNAc, but can use GDP-L-fucose as the sugar donor with almost the same efficiency as GDP-L-colitose.","Involved in the biosynthesis of 3-deoxy-D-manno-octulosonate (Kdo). Catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 3-keto-group of 2-keto-3-deoxy-D-gluconate, yielding 2,3-diketo-2-deoxy-D-gluconate (Kdo).",""
"157","CHNC_ACISS","300","0.2885","no","not-Yes","1","Equally specific","More informative","yes","Hydrolase involved in the degradation of cyclohexanol to adipate. Catalyzes the conversion of hexano-6-lactone (epsilon-caprolactone) to 6-hydroxyhexanoate (Probable).","Acyltransferase; part of the Fusarium detoxification of benzoxazolinone cluster 2 (FDB2) involved in the degradation of benzoxazolinones produced by the host plant. Maize, wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their inherent instability once released, spontaneously degrade to",""
"158","CHND_ACISS","352","0.2422","no","not-Yes","3","Less specific","Equally informative","no","Dehydrogenase involved in the degradation of cyclohexanol to adipate. Catalyzes the conversion of 6- hydroxyhexanoate to 6-oxohexanoate.","Catalyzes the NAD(P)H-dependent reduction of a range of aldehydes and ketones. In vitro also catalyzes the reduction of 4-nitrobenzaldehyde to 4-aminobenzoate.","Both describe the same reversible reaction, but different directions."
"159","LIPA_PENCY","285","0.2466","no","Yes","4","Equally specific","More informative","yes","Secreted alkaline lipase that hydrolyzes acylglycerol lipids such as triacylglycerols and consequently releases free fatty acid. Is able to hydrolyze tributyrin (1,2,3-tributyryl- glycerin).","Secreted lipase involved in Dandruff and seborrheic dermatitis (D/SD) probably via lipase-mediated breakdown of sebaceous lipids and release of irritating free fatty acids. Shows activity against monoglyceride and diglyceride substrates, but not triolein. Able to cleave oleic acid from 1,2-dioleoyl-sn-glycero-3-phosphocholine (18:2-POPC), a major component of cell membranes. Has no activity against 1,2-dioleoyl-sn-glycero-3-phosphoethanol","Both secreted lipases acting on glycerol-based lipids, but highlight different substrate specificites."
"160","BMP2_PSEL2","406","0.2131","no","not-Yes","1","Equally specific","More informative","no","Brominase involved in the biosynthesis of polybrominated aromatic organic compounds. Catalyzes three successive rounds of bromination of pyrrolyl-S-Bmp1 to produce mono-, di- and tribromopyrrolyl-S-Bmp1.","Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3- dihydroxymalonyl)-L-aspartate. Catalyzes the transfer of a phenylacetyl moiety from L-phenylacetyl-S-adenosylmethionine (PS-AdoMet) to 3,4-dihydroxybenzoate (3,4-DHB) to generate 4,5-dihydroxybenzoate (4,5-DHB). Can use L-phenylacetyl-S-adenosylmethionine (PS-A",""
"161","ZBD1_GIBM7","364","0.146","no","not-Yes","3","Equally specific","Less informative","yes","Zinc-binding dehydrogenase that acts as a genetic repressor of fumonisin production. With the ABC-type transporter ABC3, is involved in tolerance to pyrrocidines, inhibitors of growth of Gibberella moniliformis produced by the competitor fungus Sarocladium zeae. Under pyrrocidine challenge, abundant ZBD1 sequesters zinc, and decreases zinc availability in the cell, thereby inhibiting fumonisin biosynthesis via competitive inhibition of FUM21 (Probable). ZBD1 enzyme is then active and abundantly present in the cytoplasm where its enoyl reductase domain may detoxify pyrrocidine A by reducing its double-bonded lactam ring to yield pyrrocidine B, at which point the ABC-type transporter ABC3 can then effectively pump pyrrocidine B out of the cell (Probable).","Trans-enoyl reductase; part of the gene cluster that mediates the biosynthesis of oxaleimides, cytotoxic compounds containing an unusual disubstituted succinimide moiety. The first step of the pathway is provided by the HR-PKS poxF that serves in a new mode of collaborative biosynthesis with the PKS-NRPS poxE, by providing the olefin containing amino acid substrate via the synthesis of an ACP- bound dec-4-enoate. The cytochrome P450 monooxygenase poxM-catalyzed oxidation at the alpha-position creates the enzyme-bound 2-hydroxydec-4-enoyl-","Both describe trans-enoyl reductase within fungal secondary metabolism, but they describe different roles."
"162","AACB_ASPA1","375","0.2717","no","Yes","3","Equally specific","Equally informative","yes","N-methyltransferase; part of the gene cluster that mediates the biosynthesis of the diketopiperazine (DKP) aspkyncin. AacB mediates the synthesis of the unnatural amino acid N-methyl-L-alanine via methylation of L-alanine. Aspkyncin is produced by the NRPS aacA via condensation of the two unusual amino-acids N-methyl-L-alanine and L-kynurenine (L-Kyn). N- methyl-L-alanine originates from the methylation of L-alanine by the methyltransferase aacB whereas L-kynurenine is produced by the indoleamine-2,3-dioxygenase (IDO) that catalyzes the ring-opening oxidation of L-Trp to form N-formyl-l-kynurenine, followed by subsequent hydrolyzation to L-kynurenine (L-Kyn) through an endogenous L-kynurenine formamidase (Probable).","N-methyltransferase; part of the gene cluster that mediates the biosynthesis of the tetrahydroxanthone dimer secalonic acid D. The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase AacuL (Probable). The atrochrysone carboxyl ACP thioesterase AacuM then breaks the thioester bond and releases the atrochrysone carboxylic acid from AacuL (Probable). Atrochrysone carboxylic acid is decarboxylated by the decarboxylase AacuI, and oxidized by the anthrone oxygenase AacuG to yield em","Both N-methyltransferases, but differ in product and synthesis pathway."
"163","TPS10_DICPU","356","0.2008","no","not-Yes","4","More specific","Less informative","no","Terpene synthase that converts its substrate farnesyl diphosphate (FPP) into several unidentified sesquiterpenes. TPS10 also converts geranylgeranyl diphosphate (GGPP) into the diterpene beta-araneosene.","Terpene synthase that converts its substrate farnesyl diphosphate (FPP) into the sesquiterpene beta-caryophyllene.","Swissprot mentiones products as unidentified sesquiterpenes, prediction as specific sesquiterpene."
"164","CAPC_ORNRH","283","0.1835","no","not-Yes","1","Equally specific","Less informative","no","Reductase involved in the biosynthesis of capnine, a sulfonolipid present in the outer membrane of gliding Bacteroidetes and essential for gliding motility. Catalyzes the reduction of 3-dehydrocapnine to capnine.","Involved in the biosynthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Catalyzes the conversion of (S)-3-methyl-2-oxopentanoate to (S)-3-methyl-2-oxopent-4-enoate.",""
"165","VVIB_VIRVR","333","0.227","no","not-Yes","1","Equally specific","Equally informative","no","Terpene cyclase that cyclizes farnesyl diphosphate (FPP) to drim-8-ene diphosphate used by vviA as the native prenyl donor to catalyze the prenylation of D-mannitol on C-3'-OH to afford sporulositol A.","Cell division factor that enhances FtsZ-ring assembly. Directly interacts with FtsZ and promotes bundling of FtsZ protofilaments, with a reduction in FtsZ GTPase activity.",""
"166","ISRC_AMPFL","482","0.2161","no","not-Yes","3","Equally specific","Equally informative","yes","Acyltransferase; part of the gene cluster that mediates the biosynthesis of isariins (ISRs), cyclohexadepsipeptides that contain unusual nonproteinogenic amino acid-building blocks and possess a range of antiviral, antibacterial, and other activities. The isariin synthetase isrA possesses six modules but lacks an A domain in module 1. Based on the structures of ISRs, module 1 selects a high saturated polyketide as the starter unit. The PKS isrD probably generates the polyketide residue, which is activated by the CoA ligase isrB, and transferred by the acyltransferase isrC to the PCP1/T1 domain in module 1 (Probable).","O-acyltransferase; part of the gene cluster that mediates the biosynthesis of pyranonigrins, a family of antioxidative compounds. The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase that condenses 6 malonyl-CoA units to an acetyl starter unit, to form a 1,3,5-trioxotetradecane-6,8-dienyl-ACP. The enoyl reductase (ER) domain of pynA is likely to be functional during the first two rounds of polyketide chain extension, to generate the saturated C-C","Both describe acyltransferase in BGC, but differ in pathways and products."
"167","P5CR2_AMPFL","313","0.2393","no","not-Yes","3","Less specific","Less informative","yes","Pyrroline-5-carboxylate reductase involved in the biosynthesis of (3S)-methyl-L-proline, one of the building blocks od destruxins (DTXs), cyclohexadepsipeptides that contain unusual nonproteinogenic amino acid-building blocks and possess a range of antiviral, antibacterial, and other activities. The (3S)-methyl-L-proline building block is required for biosynthesis of DTXs including felinotoxins A to E, roseotoxins A to C, [3-Me-Pro] destruxin E chlorohydrin, as well as isaridins (ISDs) B and D. The 2-oxoglutarate-dependent dioxygenase detxE uses L-isoleucine as substrate to produce (4S,5S)-4-methyl-delta1-pyrroline- 5-carboxylate, whereas the pyrroline-5-carboxylate reductases 1 and/or 2 perform the reduction of (4S,5S)-4-methyl-delta1-pyrroline-5- carboxylate to (3S)-methyl-L-proline.","Oxidoreductase; part of the gene cluster that mediates the biosynthesis of the lipopeptide antibiotics leucinostatins that show extensive biological activities, including antimalarial, antiviral, antibacterial, antifungal, and antitumor activities, as well as phytotoxic. Leucinostatin A contains nine amino acid residues, including the unusual amino acid 4-methyl-L-proline (MePro), 2-amino-6-hydroxy-4-methyl-8-oxodecanoic acid (AHyMeOA), 3- hydroxyleucine (HyLeu), alpha-aminoisobutyric acid (AIB),","Both reductases and generally agree on function (although prediction is much less specific), but differ in pathway, role, substrate sub-type."
"168","MAND_DREDA","441","0.1487","no","not-Yes","2","Equally specific","Equally informative","no","Probable endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Involved in trapping nematodes.","Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.","Both describe endo-acting glycoside hydrolase, but different substrates."
"169","BAM_TRIV3","377","0.178","no","not-Yes","1","More specific","More informative","yes","Digests glycogen releasing maltose units.","Alpha-galactosidase that specifically cleaves terminal alpha-1,2-linked galactose residues from N-glycans in glycoproteins. Involved in the degradation of complex N-glycans during N-glycosylation processing. Catalyzes the hydrolysis of terminal alpha-1,2-linked galactose residues from N-glycans in glycoproteins such as alpha-dystroglycan (DAG1), laminin G domain- containing proteins (LMNG1, LMNG2, LMNG3 and LMNG4), transferrin receptor 1 (TFR1) and integrin beta-1 subunit (ITGB1).",""
"170","CEL1_SCLS1","389","0.2009","no","not-Yes","3","More specific","Equally informative","no","Endoglucanase that plays an important role in biomass degradation. Binds onto plant cell walls to participate in the hydrolysis of cellulose.","Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.","Both descriptions identify an endo-acting glycoside hydrolase targeting plant cell walls, but differ in target polymers."
"171","FGR4_GIBZE","150","0.1556","no","not-Yes","1","Equally specific","Less informative","yes","Probable cyclase; part of the gene cluster that mediates the biosynthesis of the tetraketides fugralins such as linear fugralin A and cyclic fugralin B, volatile compounds that play a role in the asexual reproductive cycle but are not involved in pathogenicity. Fugralin B is similar to fugralin A except for a cyclization between the carboxylic acid C-8 and the alcohol on C-4 resulting in a six membered lactone ring, probably catalyzed by the cyclase FGR4 (Probable). One of the key features of fugralins is the presence of a double methyl group, which is only rarely encountered in fungal secondary metabolites. As the fugralins cluster does not contain an independent methyltransferase, the PKS FGR1 is probably responsible for adding two methyl groups to the same carbon atom (Probable). The exact role of the individual cluster genes remains unknown and further work is needed to unravel the biosynthetic pathway (Probable).","Scytalone dehydratase-like protein; part of the gene cluster that mediates the biosynthesis of sordarin and hypoxysordarin, glycoside antibiotics with a unique tetracyclic diterpene aglycone structure. First, the geranylgeranyl diphosphate synthase sdnC constructs GGDP from farnesyl diphosphate and isopentenyl diphosphate. The diterpene cyclase sdnA then catalyzes the cyclization of GGDP to afford cycloaraneosene. Cycloaraneosene is then hydroxylated four times by the putative cytochrome P450 monooxygenases sdnB, sdnE, sdnF",""
"172","MPSD_MACPH","396","0.1632","no","Yes","3","Equally specific","Equally informative","yes","Diels-Alderase; part of the gene cluster that mediates the biosynthesis of macrophasetins, 3-decalinoyltetramic acids (DTAs) which feature a tetramate (pyrrolidine-2,4-dione) unit connected to a decalin fragment and that have potent bioactivities. The PKS- NRPS mpsA together with its associated enoylreductase partner mpsG incorporate one unit of acetyl-CoA, seven units of malonyl-CoA, and one unit of L-alanine to assemble the linear tetramic acid intermediate corresponding to the backbone of macrophasetins. Without the Diels-Alderase mpsD, the mpsA/G product can undergo the non-enzymatic intramolecular Diels-Alder (IMDA) reaction to generate both macrophasetin A and macrophasetin B. Catalyzed by mpsD, the linear tetramic acid intermediate is thoroughly converted to macrophasetin A via the endo-IMDA reaction in a regioselective and stereoselective manner. Finally, the cytochrome P450 monooxygenase mpsF catalyzes the hydroxylation at C20 to yield the end product macrophasetin C.","Diels-Alderase; part of the gene cluster that mediates the biosynthesis of oxaleimides, cytotoxic compounds containing an unusual disubstituted succinimide moiety. The first step of the pathway is provided by the HR-PKS poxF that serves in a new mode of collaborative biosynthesis with the PKS-NRPS poxE, by providing the olefin containing amino acid substrate via the synthesis of an ACP- bound dec-4-enoate. The cytochrome P450 monooxygenase poxM-catalyzed oxidation at the alpha-position creates the enzyme-bound 2-hydroxydec-4-","Both Diels-Alderase, agree somewhat on pathway, differ in specific roles."
"173","ARGN_STREL","337","0.152","no","not-Yes","2","Equally specific","Equally informative","yes","S-adenosyl-L-methionine-dependent methyltransferase involved in the formation of the rare amino acid 3-methylarginine (MeArg), which is incorporated into the peptidyl nucleoside antibiotic arginomycin. Transfers the methyl group from S-adenosyl-L- methionine into 5-guanidino-2-oxopentanoate acid to yield 5-guanidino- 3-methyl-2-oxopentanoate, a precursor of MeArg.","Involved in the biosynthesis of the anthracycline antibiotic daunomycin which is used in the treatment of cancer. Catalyzes the O-demethylation of 2,3- dihydroxy-1,4-naphtoquinone (2,3-DHNQ) to yield 2,3-dihydroxy-1,4-naphtoquinone (2,3-DHNQ). Also able to catalyze the O-methylation of 2,3-DHNQ to yield 2,3-dimethoxy-1,4-naphtoquinone (2,3-DM","Both SAM-dependent methyltransferases, but Swissprot describes C-methylation, and prediction describes O-methylation/demethylation (different function and role)."
"174","CL12B_MYCTU","151","0.2313","no","not-Yes","2","Equally specific","Equally informative","no","Probable cellulase. Can hydrolyze barley beta-glucan in vitro. Could be important for the survival of M.tuberculosis in the environment, perhaps in amoebal hosts.","Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose.","Both describe endo-acting glycoside hydrolases acting on plant cell walls, but describe different specific targets."
"175","3BHD_MYCNE","366","0.2204","no","not-Yes","1","More specific","More informative","yes","Catalyzes the degradation of testosterone into androstenedione.","NADP-dependent dehydrogenase; part of the gene cluster that mediates the biosynthesis of the tetrahydropyranyl antifungal agent restricticin that acts as an inhibitor of CYP51 and blocks the ergosterol biosynthesis. The highly reducing polyketide synthase rstn3, the short chain dehydrogenase rstn4, the cyclase rstn5, the FAD-dependent monooxygenase rstn6 and the enoylreductase rstn7 are required to generate the first stable intermediate desmethylrestrictinol. Rstn3 with rstn7 biosynthesize the first polyketide chain intermediate that is reduced by rstn4, followed by",""
"176","LTBC_ASPLT","285","0.1596","no","Yes","3","More specific","Equally informative","yes","Probable methyltransferase; part of the gene cluster that mediates the biosynthesis of luteodienoside A, a glycosylated polyketide consisting of an unusual 1-O-beta-D-glucopyranosyl-myo- inositol (glucinol) ester of 3-hydroxy-2,2,4-trimethylocta-4,6-dienoic acid. The HR-PKS ltbA produces the trimethylated polyketide chain from acetyl-CoA, malonyl-CoA and S-adenosylmethionine (SAM), and the ltbA cAT domain then uses glucinol produced by the glycosyltransferase ltbB as an offloading substrate to release luteodienoside A. Since ltbA and ltbB are sufficient for the biosynthesis of luteodienoside A, the functions of the methyltransferase ltbC and the FAD-binding monooxygenase ltbD within the pathway remain obscur.","Probable O-methyltransferase; part of the gene cluster that mediates the biosynthesis of pyranonigrins, a family of antioxidative compounds. The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase that condenses 6 malonyl-CoA units to an acetyl starter unit, to form a 1,3,5-trioxotetradecane-6,8-dienyl-ACP. The enoyl reductase (ER) domain of pynA is likely to be functional during the first two rounds of polyketide chain extension, to generate the saturated C-","Both are methyltransferases with same substrate class, but differ in role and pathway."
"177","DHGRD_NOVAD","304","0.1976","no","not-Yes","1","Equally specific","Less informative","no","Part of the alkanesulfonate monooxygenase (sulfo-ASMO) pathway, a D-sulfoquinovose degradation pathway that enables the complete utilization of all carbons within sulfoquinovose (SQ) with concomitant production of inorganic sulfite (Ref.2). Catalyzes the NADP-dependent reduction of 6-dehydro-D-glucose to D-glucose (Ref.2). Can also catalyze the reversible reaction, the formation of 6-dehydro- D-glucose from D-glucose in the presence of NADP(+) (Ref.2).","Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the transfer of the methyl group from S-adenosyl-L- methionine to the aldehyde group of betaine aldehyde and is thus involved in the final step in the biosynthesis of glycine betaine.",""
"178","PNBA_PSEPU","227","0.2067","no","not-Yes","2","Equally specific","More informative","yes","Nitroreductase involved in the degradation of nitroaromatic compounds. Catalyzes the conversion of 4- nitrobenzoate to 4-hydroxylaminobenzoate. Required for the catabolism of 4-nitrotoluene.","Component of the naphthalene dioxygenase system, which catalyzes the O2-dependent cleavage of the C-C bond in the naphthalene ring system to yield catechol as a product. In vitro, can also catalyze the O2-dependent cleavage of 1,4-naphthoquinone (1,4-NQ) and 2,3-dihydroxynaphthalene (2,3-DHN), but not of 1,2-dihydroxynaphthalene (1,2-DHN), 1,3-dihydroxynaphthalene (1,3-DHN), 1","Both describe oxidoreductases acting on aromatic compounds, but describe different functions, substrates, roles, and pathways."
"179","PRIA_KLEP7","731","0.7157","yes","Yes","5","Equally specific","Equally informative","no","Initiates the restart of stalled replication forks, which reloads the replicative helicase on sites other than the origin of replication. Recognizes and binds to abandoned replication forks and remodels them to uncover a helicase loading site. Promotes assembly of the primosome at these replication forks.","Initiates the restart of stalled replication forks, which reloads the replicative helicase on sites other than the origin of replication. Recognizes and binds to abandoned replication forks and remodels them to uncover a helicase loading site. Promotes assembly of the primosome at these replication forks.","Exact similar text."
"180","PLK_GIAIC","678","0.7459","yes","not-Yes","3","More specific","Equally informative","yes","Involved in cell cycle. Involved in cell division. Involved in cytokinesis. Involved in flagella biogenesis and in regulation of flagella length in interphase. Involved in formation of median bodies during interphase. Phosphorylates Kin-13 in vitro. Likely regulates microtubule (MT) depolymerizing activity of Kin-13.","Serine/threonine-protein kinase that plays a role in a variety of mitotic and meiotic processes such as chromosome condensation, sister chromatid segregation, homologous recombination, DNA damage repair and meiosis I. Required for efficient homologous recombination repair of double-strand breaks (DSBs) at meiosis I by mediating phosphorylation of the RAD51 recombinase and RIF1, an essential component of the meiotic recombination machinery. Also required for sister chromatid segregation during meiosis I by mediating phosphorylation of the MAD2L1 subunit of the anaphase-promoting complex (APC/C), promoting the degradation of MAD2L1 by the APC","Both describe kinase in cell division, but Swissprot describes cytokinesis, and prediction chromosome condensation. (Different roles, similar context)"
"181","AMFR_DANRE","620","0.9112","yes","Yes","4","Equally specific","Less informative","no","E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins. May participate in the final step of endoplasmic reticulum-associated degradation (ERAD). Required for proper lipid homeostasis.","E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.","Both describe E3 ubiquitin-protein ligase, Swissprot just adds substrate specificity."
"182","KAPB_ARTOA","519","0.7177","yes","not-Yes","2","More specific","More informative","yes","Functions downstream of adenylate cyclase to regulate trap- development for nematode capture.","Serine/threonine-protein kinase involved in the regulation of cell growth, proliferation and survival. Phosphorylates and activates MAP2K1/MKK1 and MAP2K2/MKK2, leading to activation of the MAP kinase/ERK (MAPK/ERK) signaling pathway. Phosphorylates and activates MAP3K5/ASK1, leading to activation of the MAPK/ERK signaling pathway. Phosphorylates and activates MAP3K7/ASK2, leading to activation of the MAPK/ERK signaling pathway. Phosphorylates and activates MAP3K8/JNK1, leading to","Both describe kinases that act in signal transduction, but describe entirely different signaling pathways."
"183","TRI55_MOUSE","545","0.8826","yes","Yes","3","Equally specific","Equally informative","yes","E3 ubiquitin ligase that plays an important role in regulating cardiac development and contractility, muscle growth, metabolism, and fiber-type differentiation. Acts as a critical factor that regulates cardiomyocyte size during development in concert with TRIM63 by regulating E2F1-mediated gene expression. Plays a role in apoptosis induction in cardiomyocytes by promoting ubiquitination of the DUSP1 phosphatase. Promotes non-canonical NF-kappa-B signaling and B-cell-mediated immune responses by mediating NFKB2 'Lys-48'-linked ubiquitination and processing. In turn, NFKB2 is further processed by valosin-containing protein/VCP, an ATPase that mediates ubiquitin-dependent protein degradation by the proteasome. May play a role in preventing macrophages from producing inflammatory factors and migrating by downregulating the level of nuclear NF-kappa-B subunit RELA. Modifies also PPARG via polyubiquitination and accelerates PPARG proteasomal degradation to inhibit its activity.","E3 ubiquitin-protein ligase that plays a role in the regulation of innate immunity and inflammatory response. Acts as a positive regulator of the NLRP3 inflammasome by mediating 'Lys-48'-linked polyubiquitination and proteasomal degradation of NLRC4. Acts as a negative regulator of the cGAS-STING pathway by promoting 'Lys- 63'-linked polyubiquitination and degradation of STING1. Acts as a positive regulator of NF-kappa-B signaling by mediating 'Lys-48'-linked polyubiquitination and degradation of RELA. Acts as a negative regulator","Both describe E3 ubiquitin ligase, but different substrates and contexts (cardiac development vs. immunity). Share general category and mechanism but disagree on role."
"184","CMT1_TRYB2","324","0.7372","yes","not-Yes","3","Equally specific","Less informative","no","Recapping enzyme that methylates the guanine N7 position of hypermethylated GpppRNA to regenerate a mature cap structure on decapped mRNA. Can also methylate GpppG and GpppA; the RNA component is not strictly essential for catalysis.","S-adenosyl-L-methionine-dependent protein-lysine N- methyltransferase that methylates elongation factor 1-alpha (EF1-alpha) at 'Lys-37'.","Both are SAM-dependent methyltransferases, but Swissprot describes a nucleoside/nucleic acid methyltransferase, and prediction a protein-lysine methyltransferase. Disagreement on role, rather than function."
"185","MARC3_CAEEL","431","0.7933","yes","Yes","3","Equally specific","Equally informative","no","E3 ubiquitin-protein ligase which positively regulates the fast polyspermy block during fertilization, preventing entry of more than one sperm into the oocyte. After fertilization, required in the zygote for the selective degradation of a subset of maternal membrane proteins including cav-1, chs-1 and rme-2, probably by mediating their K63-linked polyubiquitination.","E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of E74 alpha/RNF43, a component of the PRC1 polycomb group (PcG) complex. Plays a role in the negative regulation of T-cell receptor (TCR)- mediated activation of NF-kappa-B.","Both describe E3 ubiquitin ligase, but different roles and downstream signals."
"186","CBL_CHICK","903","0.7924","yes","Yes","4","More specific","More informative","yes","E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Involved in regulation of apoptosis in duodenal epithelial cells.","Protein-protein E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in an ATP-dependent manner and is involved in protein degradation through ubiquitination and subsequent proteasomal degradation of target proteins. Plays a role in the negative regulation of the Ras/MAPK signaling pathway. Plays a role in Ras-induced apoptosis. Plays a role in the negative regulation of the Ras/MAPK signaling pathway. Plays a role in the negative regulation of the Ras/MAPK signaling pathway. Plays a role in the negative regulation of the Ras/MAPK signaling pathway. Plays a role in the negative regulation of the","Prediction describes specific mechanism, while Swissprot does not. Repeated description in prediction text."
"187","DPP9_DANRE","885","0.7119","yes","not-Yes","4","Less specific","Less informative","no","Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Acts as a key inhibitor of the NLRP1 inflammasome.","Dipeptidyl-peptidase-like protein which may play a role in the regulation of cell wall integrity.","Swissprot description includes exact mechanism, while prediction does not."
"188","ZN488_DANRE","502","0.7359","yes","not-Yes","4","Less specific","Equally informative","no","Transcriptional repressor. May have histone methyltransferase activity (Probable). Negatively regulates shh signaling activity in pMN progenitor cells which prevents their switch from motor neuron to oligodendrocyte precursor cell production. Independently of shh activity, also regulates oligodendrocyte formation.","Probable regulator of transcription. Has been implicated in a variety of biological processes, including cell proliferation, differentiation and apoptosis, and in the regulation of the glucocorticoid receptor (NR3C1). May inhibit the transcriptional activity of EP300 by competing for the same binding site on EP300.","Same mechanism, differ in downstream targets."
"189","RGYRA_NANEQ","701","0.7097","yes","Yes","3","Equally specific","Less informative","yes","Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. Binds to single-stranded DNA, transiently cleaves and then rejoins the end, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Probably involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair or for DNA protection (Probable). Reconstituted holoenzyme binds dsDNA a bit better than ssDNA, this subunit preferentially binds ssDNA. In isolation this subunit relaxes negatively-supercoiled DNA, and stimulates the endogenous ATPase activity of the RgyB subunit.","Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally,","Both describe the same mechanism, but Swissprot mentions introducing a positive supercoil and prediction removing DNA supercoils."
"190","DDX31_PLAF7","899","0.7198","yes","not-Yes","4","More specific","Equally informative","no","Has DNA helicase activity and may also have RNA helicase activity; the DNA helicase direction was not determined. Shows ssDNA and RNA dependent ATPase activity.","ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.","Both describe ATP-dependent nucleic acid helicase, but Swissprot additionally describes DNA helicase activity."
"191","TOP3_SACS2","668","0.7598","yes","Yes","4","Equally specific","Less informative","yes","Relaxes negatively supercoiled (SC) DNA in an ATP-independent manner at 75 degrees Celsius. From 50-85 degrees Celsius relaxes negatively SC DNA; at up to 100 degrees Celsius it unlinks catenated DNA to form circular double-stranded (ds)DNA molecules. The promotion or prevention of DNA linking/unlinking is temperature-dependent in vitro and probably depends on other factors in vivo. One study found single-stranded binding protein (SSB) does not enhance its activity , while another study found it does. Binds single-stranded (ss)DNA and less well to Holliday junction (HJ) or forked DNA. Anneals complementary oligonucleotides to form HJ structures.","Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally,","Both describe type I DNA topoisomerase, but prediction describes the mechanism, and Swissprot describes in vitro activity."
"192","UBA7_MOUSE","977","0.7832","yes","not-Yes","3","Less specific","Equally informative","yes","E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferons stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation. Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus or rotavirus.","E1-like enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy and pexophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E1-like reaction of the ATG12-ATG5 conjugation system that forms the ATG10-ATG12 complex: the ATG12-ATG5 conjugate functions as an E1-like enzyme that mediates the ATP-dependent transfer of ATG10","Both describe the same function, but differ in specific roles."
"193","PSUG_DICD3","316","0.799","yes","Yes","5","Equally specific","Less informative","no","Catalyzes the reversible cleavage of pseudouridine 5'- phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway. Part of an operon that could be involved in the biosynthesis of the blue pigment indigoidine, which is implicated in pathogenicity and protection from oxidative stress.","Catalyzes the reversible cleavage of pseudouridine 5'- phosphate (PsiMP) to ribose 5-phosphate and uracil. Functions biologically in the cleavage direction, as part of a pseudouridine degradation pathway.","Exactly similar description, but Swissprot description includes additional statement broader biological context."
"194","RECG_THEMA","780","0.7675","yes","Yes","5","Equally specific","Less informative","no","Plays a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has replication fork (Y-DNA) regression activity, unwinds stalled or blocked replication forks to make a HJ that can be resolved. Has a DNA unwinding activity characteristic of a DNA helicase with 3'-5' polarity. Might be a DNA translocase rather than a bona fide helicase (Probable).","Plays a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has replication fork regression activity, unwinds stalled or blocked replication forks to make a HJ that can be resolved. Has a DNA unwinding activity characteristic of a DNA helicase with 3'-5' polarity.","Exactly similar description, but Swissprot description includes additional statement on probable role."
"195","NLEG1_CITRO","194","0.7659","yes","Yes","4","Less specific","Less informative","no","Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is probably an E3 ubiquitin-protein ligase that interferes with the host's ubiquitination pathway and targets host proteins for proteasomal degradation (Probable). Mice infected with a strain of bacteria deleted for this gene have less severe diarrhea.","Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin-protein ligase that probably targets host proteins for ubiquitination.","Swissprot describes it to target host proteins for degradation, while prediction describes it to target host proteins for ubiquitination."
"196","DUBU3_SIMNZ","743","0.7345","yes","not-Yes","2","Equally specific","Less informative","no","Effector protein secreted into the host cytoplasm that counteracts ubiquitin-based antibacterial defenses. Shows deubiquitinase activity with ubiquitin model substrates but is hardly active against di-ubiquitin chains, meaning that this enzyme might be a substrate-directed deubiquitinase rather than a chain-cleaving enzyme.","E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.","Swissprot describes DUB, while prediction describes ubiquitin ligase. Prediction does not explicitly negate Swissprot activity, but asserts the reverse function."
"197","NPRS_SCHMA","1227","0.2906","no","not-Yes","1","Equally specific","Equally informative","no","Catalyzes the condensation of beta-alanine with tryptamine to form beta-alanyl-tryptamine (BATT). Beta-alanyl- tryptamine is an essential pheromone produced by the male that stimulates female sexual development during pairing.","Acts as a receptor for the polyketide synthase (PKS)-derived fungal secondary metabolite fusicoccin (FUS). Binds FUS with high affinity and mediates its nuclear localization via interaction with importin alpha. May play a role in FUS biosynthesis or regulation.",""
"198","HDMA_ASPFU","1081","0.2229","no","not-Yes","2","More specific","More informative","no","H3K4 demethylase-like protein. Might not act as a H3K4 demethylase or is not the major H3K4 demethylase since its deletion does not affect whole genome H3K4 methylation.","Histone methyltransferase that trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.","Swissprot mentions ""demethylase-like"", and prediction mentions ""methyltransferase"". Swissprot also mentions ""might not act as H3K4 demethylase,"" so does not explicitly contradict prediction."
"199","PLYL_THEST","1240","0.2983","no","not-Yes","2","Equally specific","Less informative","no","Cleaves polygalacturonate or partially methylated pectin. When assayed on polygalacturonate or on pectin, it releases monogalacturonate as the principal product.","Lectin that specifically recognizes and binds to the non-reducing end of poly-beta-1,6-D-glucuronic acid.","Swissprot describes cleaving enzyme, and prediction describes a lectin (binding protein). Both describe same substrate."
"200","TAS1_BOTB1","1711","0.206","no","not-Yes","3","Equally specific","Equally informative","yes","Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein. TAS1 alone is sufficient for TeA synthesis via the condensation of isoleucine (Ile) with acetoacetyl-CoA by the N-terminal NRPS module and subsequent cyclization conducted by the C-terminal KS domain.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe polyketide synthase (share function and core chemistry), but differ in specific roles (Swissprot describes TAS1, prediction SAT)."
"201","STMB_ASPUT","2059","0.2398","no","not-Yes","3","Equally specific","Equally informative","yes","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of stromemycin, a depside C-glucoside with two unsaturated C9 side chains belonging to aromatic polyketide glycosides. The HR-PKS stmA and the NR-PKS stmB act as scaffold-generating enzymes responsible for the biosynthesis of the polyketide skeleton bininalkenylresorcylic acid. StmA condenses on acetyl-CoA starter unit with 4 malonyl-CoA units and the stmB uses 3 more malonyl-CoA units and catalyzes the depside bond formation. The glycoytransferase stmC then acts as the tailoring enzyme responsible for 3-C-glucosylation of bininalkenylresorcylic acid to yield stromemycin.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe NR-PKS, but describe synthesis of different products."
"202","STMA_ASPUT","2367","0.2611","no","not-Yes","3","Equally specific","Equally informative","yes","Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of stromemycin, a depside C-glucoside with two unsaturated C9 side chains belonging to aromatic polyketide glycosides. The HR-PKS stmA and the NR-PKS stmB act as scaffold-generating enzymes responsible for the biosynthesis of the polyketide skeleton bininalkenylresorcylic acid. StmA condenses on acetyl-CoA starter unit with 4 malonyl-CoA units and the stmB uses 3 more malonyl-CoA units and catalyzes the depside bond formation. The glycoytransferase stmC then acts as the tailoring enzyme responsible for 3-C-glucosylation of bininalkenylresorcylic acid to yield stromemycin.","Highly reducing polyketide synthase (HR-PKS); part of the gene cluster that mediates the biosynthesis of the indole diterpenes penitrems. Within the pathway, ptmC is responsible for the cyclization of prepenitrem D to yield penitrem B. The geranylgeranyl diphosphate (GGPP) synthase ptmG catalyzes the first step in penitrem biosynthesis via conversion of farnesyl pyrophosphate and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP). Condensation of indole-3-glycerol phosphate with GGPP by ptmC then forms 3","Both describe HR-PKS, but describe synthesis of different products."
"203","PKS1_AURME","2190","0.2262","no","not-Yes","3","Equally specific","Equally informative","yes","Highly-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of liamocins, glycolipids (also called heavy oils) composed of a single mannitol or arabitol headgroup linked to either three, four or even six 3,5-dihydroxydecanoic ester tail- groups. Within the pathway, PKS1 is responsible for biosynthesis of 3,5-dihydroxydecanoic acid from acetyl-CoA and malonyl- CoA. A phosphopantetheine transferase (PPTase) activates the HR-PKS. The esterase EST1 then catalyzes ester bond formation between 3,5-dihydroxydecanoic acid and mannitol (provided by the mannitol-1-phosphate 5-dehydrogenase and the NADP-dependent mannitol dehydrogenase) or arabinol (provided by the L- arabinitol 4-dehydrogenase).","Highly reducing polyketide synthase (HR-PKS); part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the at","Both describe HR-PKS, but describe synthesis of different products."
"204","VERA_PENPO","2566","0.2726","no","not-Yes","3","Equally specific","Less informative","yes","Highly reducing polyketide synthase (HR-PKS); part of the gene cluster that mediates the biosynthesis of the neurotoxin verrucosidin, a methylated alpha-pyrone polyketide that inhibits oxidative phosphorylation in mitochondria and thereby causes neurological diseases. The carbon backbone of verrucosidin is synthesized by the HR-PKS verA, and further modified by the other verrucodidin cluster enzymes (Probable).","Highly reducing polyketide synthase (HR-PKS); part of the gene cluster that mediates the biosynthesis of the indole diterpenes penitrems. Within the pathway, ptmC is responsible for the cyclization of prepenitrem D to yield penitrem B. The geranylgeranyl diphosphate (GGPP) synthase ptmG catalyzes the first step in penitrem biosynthesis via conversion of farnesyl pyrophosphate and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP). Condensation of indole-3-glycerol phosphate with GGPP by ptmC then forms 3","Both describe HR-PKS, but describe synthesis of different products."
"205","HASD_ASPFC","2273","0.2644","no","not-Yes","3","Less specific","Less informative","yes","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of hexadehydro-astechrome (HAS), a tryptophan-derived iron(III)-complex that acts as a virulence factor in infected mice. Within the pathway, the NRPS condenses tryptophan and alanine to produce the Trp-Ala dipeptide. The 7-dimethylallyltryptophan synthase hasE then catalyzes the prenylation of the hasD-tethered tryptophan or the resulting tethered Trp-Ala dipeptide at the C-7 position of the indole moiety. HAS biosynthesis continues via tethered intermediates with the succesive actions of the cytochrome P450 monooxygenase hasH, the O- methyltransferase hasC, and the FAD-linked oxidoreductase hasG. The resulting O-methylated diketopiperazine is then released from hasD. Finally, three O-methylated diketopiperazine molecules assemble in a trimeric complex with Fe(III) to produce hexadehydro-astechrome.","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of squalestatin S1 (SQS1, also known as zaragozic acid A), a heavily oxidized fungal polyketide that offers potent cholesterol lowering activity by targeting squalene synthase (SS). SQS1 is composed of a 2,8- dioxobicyclic[3.2.1]octane-3,4,5-tricarboxyclic acid core that is connected to two lipophilic polyketide arms. These initial steps feature the priming of an unusual benzoic acid starter unit onto","Both describe NRPS, but describe synthesis of different products."
"206","NORB_STRON","2174","0.2291","no","not-Yes","3","Equally specific","Equally informative","no","Component of a type I modular polyketide synthase (PKS) that generates the backbone of the antibiotic spectinabilin (also known as neoaureothin), a nitroaryl-substituted polyketide metabolite. This PKS system accepts the unusual starter unit 4- nitrobenzoyl-CoA and extends it by 6 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA.","Involved in the biosynthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. The first step in bacillaene biosynthesis is the formation of the polyketide backbone by condensation of one acetyl-CoA starter unit with 7 malonyl-CoA units. Polyketides are derived from acetyl-CoA in bacillaene biosynthesis.","Swissprot describes cis-AT PKS (spectinabilin synthesis), while prediction describes trans AT PKS (bacillaene synthesis)."
"207","NORC_STRON","2266","0.2672","no","not-Yes","2","Equally specific","More informative","yes","Component of a type I modular polyketide synthase (PKS) that generates the backbone of the antibiotic spectinabilin (also known as neoaureothin), a nitroaryl-substituted polyketide metabolite. This PKS system accepts the unusual starter unit 4- nitrobenzoyl-CoA and extends it by 6 molecules of (S)-methylmalonyl-CoA and a single molecule of malonyl-CoA.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Swissprot describes cis-AT PKS (spectinabilin synthesis), while prediction describes NR-PKS. Both are Type I polyketide synthases, but differ in function."
"208","SIDD_BEAB2","1843","0.2365","no","not-Yes","3","Equally specific","Equally informative","yes","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of at least 11 siderophores, including beauverichelin A, dimerumic acid (DA), Na-dimethyl coprogen (NADC), eleutherazine B, ferricrocin (FC), fusarinine A, fusarinine C (FsC), metachelin A, mevalonolactone, rhodotorulic acid (RA) and tenellin. This cocktail of siderophores for iron metabolism is essential for virulence, and more specifically for the fungal virulence in penetrating through the host cuticle. Siderophore synthesis is also involved in conidial germination under iron-deficient conditions. SIDC catalyzes the assembly of ferricrocin whereas SIDD catalyzes the assembly of fusarinine C.","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties. L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism. The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by","Both describe NRPS, but describe synthesis of different products."
"209","PREU3_PREIS","2523","0.2991","no","not-Yes","3","More specific","Less informative","yes","Non-reducing polyketide synthase; part of a gene cluster that mediates the biosynthesis of a yet unidentified natural product (Probable). The first step in the pathway is performed by Preu3 that condenses one acetyl-CoA starter unit with 3 malonyl-CoA units. Preu3 also catalyzes one methylation step to produce 3-methylorsellinate, an intermediate that exhibits significant antibacterial activities against methicillin-resistant Staphylococcus aureus, multidrug-resistant Enterococcus faecalis, multidrug-resistant Enterococcus faecium, and multidrug-resistant Staphylococcus epidermidis.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe NR-PKS, but describe synthesis of different products."
"210","PKS91_EMENI","2568","0.2406","no","not-Yes","3","More specific","Equally informative","no","Highly reducing polyketide synthase; part of a cluster that mediates the biosynthesis of a yet undetermined secondary metabolite (Probable). With esterase AN6793, produces a pathway intermediate compound with molecular weight 258.","Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the mycotoxin fusarin C. Within the cluster, FUS1, FUS2, FUS8 and FUS9 are sufficient for fusarin production. The other FUS cluster members are not essential for fusarin C biosynthesis.","Both describe NR-PKS, but describe synthesis of different products."
"211","AGAO_MICTH","1175","0.2534","no","not-Yes","3","More specific","More informative","yes","Endo-type beta-agarase, which degrades agarose and agarose oligosaccharides more polymerized than hexamers to yield neoagarohexaose (NA6) as the main product, with lesser amounts of neoagarotetraose (NA4) and neoagarobiose (NA2).","Catalyzes the endohydrolysis of the (1->3)-alpha-L-glucosidic linkages in alpha-L-rhamnogalacturonan I (RG-I), a major component of plant cell walls. RG-I is one of the most abundant cell wall polysaccharides in plants and is composed of alpha-(1->4)-linked rhamnogalacturonan I (RG-I) and alpha-(1->3)- and alpha-(1->4)-linked rhamnogalacturonan II (RG-II). RG-","Both are endoglycoside hydrolases that cleave internal glycosidic links, but disagree on the type of polysaccharide and substrates."
"212","HYLA_ENTFA","1372","0.2391","no","not-Yes","2","More specific","Less informative","no","Has a very modest degradation activity against heparin sodium salt (HS) in vitro. Involved in the pathogenesis of vancomycin- resistant E.faecalis infections.","Polysaccharide lyase that catalyzes the endolytic cleavage of beta-1,4-glucosidic linkages in laminarin and other beta-1,3-D-glucan substrates. Can also hydrolyze the beta-1,3-D-glucosidic linkages in carboxymethylcellulose (CMC) and in the synthetic substrate 4-methylumbelliferyl-beta-D-glucopyranoside (MUG).","Both degrade polysaccharides, but do not describe any further shared reaction or mechanism. Shared function is overly general."
"213","CTSY_SPOGL","1093","0.2217","no","not-Yes","1","Equally specific","Equally informative","no","Glycosyltransferase involved, together with CtsZ, in the conversion of alpha-1,4-glucan into a cyclic tetrasaccharide (CTS) constructed from four alpha-glucopyranosyl residues. Catalyzes the alpha-(1->3) transfer of the isomaltosyl moiety of alpha-isomaltosyl-(1->4)-D-maltotriose to another alpha-isomaltosyl-(1->4)-D-maltotriose, resulting in alpha-isomaltosyl- (1->3)-alpha-isomaltosyl-alpha-(1->4)-maltotriose formation. In addition, the enzyme catalyzes the intramolecular cyclization of the product, generating the cyclic tetrasaccharide cyclobis-(1->6)-alpha-nigerosyl.","Alpha-glucosidases, also called alpha-1,4-glucanases or alpha-1,3-glucanases, cleave the non-reducing end alpha-1,3-linked glucosyl residues from the non-reducing end of glucans. They play a role in the breakdown of certain alpha-glucans present in the diet, for example the disaccharide sucrose.","While both act on alpha-glucans, Swissprot describes building (transferase), while preiction describes breaking down (hydrolase). Explicit contradictions."
"214","TOXH_COCCA","1547","0.2359","no","not-Yes","3","Less specific","Equally informative","yes","Fatty acid synthase alpha subunit, part of the diffuse TOX2 gene cluster that mediates the biosynthesis of the HC-toxin, cyclic tetrapeptide of structure cyclo(D-Pro-L-Ala-D-Ala-L-Aeo), where Aeo stands for 2-amino-9,10-epoxi-8-oxodecanoic acid. HC- toxin is a determinant of specificity and virulence in the interaction between the producing fungus and its host, maize. TOXH contribute to the synthesis of the decanoic backbone of 2- amino-9,10-epoxi-8-oxodecanoic acid, an essential precursor for the production of the major forms of HC-toxin by the non-ribosomal peptide synthetase HTS1 (Probable).","Fatty acid synthase alpha subunit; part of the gene cluster that mediates the biosynthesis of squalestatin S1 (SQS1, also known as zaragozic acid A), a heavily oxidized fungal polyketide that offers potent cholesterol lowering activity by targeting squalene synthase (SS). SQS1 is composed of a 2,8- dioxobicyclic[3.2.1]octane-3,4,5-tricarboxyclic acid core that is connected to two lipophilic polyketide arms. These initial steps feature the priming of an unusual benzoic acid starter unit","Both sFAS subunits, but describe synthesis of different products."
"215","TAS1_GLOTA","1558","0.2607","no","not-Yes","3","Equally specific","Equally informative","yes","Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein. TAS1 alone is sufficient for TeA synthesis via the condensation of isoleucine (Ile) with acetoacetyl-CoA by the N-terminal NRPS module and subsequent cyclization conducted by the C-terminal KS domain.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe polyketide synthase (share function and core chemistry), but differ in specific roles (Swissprot describes TAS1, prediction SAT)."
"216","LYS11_PENEN","1116","0.2731","no","Yes","4","Equally specific","More informative","no","Probable chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Might be involved in manipulation of host defenses for successful infection (Probable).","Secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Participates in the infection process and directly acts in the penetration process of the host cuticle.",""
"217","PKS39_CYTMA","2584","0.2494","no","not-Yes","4","More specific","More informative","yes","Highly reducing polyketide synthase; part of the biosynthetic gene cluster 39 that is not involved in virulence.","Highly reducing polyketide synthase (HR-PKS); part of the gene cluster that mediates the biosynthesis of the indole diterpenes penitrems. Within the pathway, ptmJ catalyzes the last step by converting the 13,14-epoxide of penitrem C to yield penitrem A. The geranylgeranyl diphosphate (GGPP) synthase ptmG catalyzes the first step in penitrem biosynthesis via conversion of farnesyl pyrophosphate and isopentyl pyrophosphate into geranylgeranyl pyrophosphate (GGPP). Condensation of indole-3-glycerol phosphate with","Same enzyme, prediction provides specific context/pathway."
"218","AACA_ASPA1","1733","0.2978","no","not-Yes","3","Less specific","Equally informative","yes","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of the diketopiperazine (DKP) aspkyncin. AacA catalyzes the condensation of two unusual amino-acids, N-methyl-L-alanine and L-kynurenine (L-Kyn), via the nucleophilic attack of the secondary amine group of N-methyl-L-alanine on the thioester of N-methyl-L-alanine-L-Kyn-S-T2, resulting in the formation of aspkyncin. N-methyl-L-alanine originates from the methylation of L-alanine by the methyltransferase aacB whereas L-kynurenine is produced by the indoleamine-2,3-dioxygenase (IDO) that catalyzes the ring-opening oxidation of L-Trp to form N-formyl-l- kynurenine, followed by subsequent hydrolyzation to L-kynurenine (L- Kyn) through an endogenous L-kynurenine formamidase (Probable).","Nonribosomal peptide synthetase; part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties. L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism. The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by","Same enzyme, different pathways and products."
"219","HIPS_NEONM","1678","0.217","no","not-Yes","3","Equally specific","Less informative","yes","PKS-NRPS hybrid synthetase; part of the gene cluster that mediates the fungal bioluminescence cycle. Performs the biosynthesis of hispidin from caffeic acid by two cycles of addition of malonyl units followed by lactonization. The fungal bioluminescence cycle begins with the hispidin synthetase that catalyzes the formation of hispidin which is further hydroxylated by the hispidin-3-hydroxylase, yielding the fungal luciferin 3- hydroxyhispidin. The luciferase then produces an endoperoxide as a high-energy intermediate with decomposition that yields oxyluciferin (also known as caffeoylpyruvate) and light emission. Oxyluciferin can be recycled to caffeic acid by caffeoylpyruvate hydrolase (Probable).","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both extend polyketide chains, but Swissprot describes hybrid PKS-NRPS and prediction describes NR-PKS (distinct sub-classes)."
"220","EXGAL_BIFL2","1513","0.2789","no","not-Yes","2","Equally specific","Less informative","yes","Involved in the type II arabinogalactan (AG) side chains degradation. Specifically releases the non-reducing terminal beta-1,6-galactobiose (beta-1,6-Gal2) from both dearabinosylated larch AG and polymeric beta-1,6-galactan chains by an exo-mode of action. Shows lower activity with larch AG, and very weak activity with dearabinosylated gum arabic, gum arabic and potato galactan. Can probably release beta-1,6- Gal2 from the internal side chains of type II AG.","Involved in the degradation of galactomannan. Catalyzes the release of beta-D-galactofuranosyl residues from alpha-D-galactofuranosyl-beta-D-mannopyranosyl (1->6)-beta-D-glucopyranosyl-(1->4)-alpha-D-galactofuranosyl-(1->6)-beta-D-mannopyranosyl (1->6)-beta-D-glucopyranosyl-(1->4)-alpha-D-galactofuranosyl-(1->6)-beta-D","Both describe glycoside hydrolases, but different sub-categories (Swissprot is galactopyranosidase, prediction is galactofuranosidase)."
"221","ISRD_AMPFL","2544","0.2692","no","not-Yes","3","Equally specific","Less informative","yes","Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of isariins (ISRs), cyclohexadepsipeptides that contain unusual nonproteinogenic amino acid-building blocks and possess a range of antiviral, antibacterial, and other activities. The isariin synthetase isrA possesses six modules but lacks an A domain in module 1. Based on the structures of ISRs, module 1 selects a high saturated polyketide as the starter unit. The PKS isrD probably generates the polyketide residue, which is activated by the CoA ligase isrB, and transferred by the acyltransferase isrC to the PCP1/T1 domain in module 1 (Probable).","Highly reducing polyketide synthase (HR-PKS); part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the at","Both describe HR-PKS, but describe synthesis of different products."
"222","GDE_TRIV3","1469","0.2595","no","not-Yes","3","More specific","Equally informative","no","Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation. Exhibits debranching activity towards phosphorylase limit dextrin.","D-maltase involved in the degradation of maltodextrins. Catalyzes the reversible hydrolysis of (1->4)-alpha-D-glucosidic bonds in alpha-D- and alpha-L-maltose, alpha-D- and alpha-D-malto-oligomers, and alpha-D- and alpha-D-malto-tetramers. Is also able to hydrolyze alpha-D- and alpha-D-malto-oligomers with a degree of polymerization (DP) higher than 4.","Both describe enzymes that degrade alpha-glucans, but different sub-categories (Swissprot is debranching enzyme, prediction is D-maltase)."
"223","TAS1_CORMM","1582","0.1868","no","not-Yes","3","Equally specific","Equally informative","yes","Hybrid PKS-NRPS synthetase that mediates the biosynthesis of the toxin tenuazonic acid (TeA), an inhibitor of protein biosynthesis on ribosomes by suppressing the release of new protein. TAS1 alone is sufficient for TeA synthesis via the condensation of isoleucine (Ile) with acetoacetyl-CoA by the N-terminal NRPS module and subsequent cyclization conducted by the C-terminal KS domain.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe polyketide synthase (share function and core chemistry), but differ in specific roles (Swissprot describes TAS1, prediction SAT)."
"224","PREU6_PREIS","2197","0.2197","no","Yes","3","Equally specific","Less informative","yes","Non-reducing polyketide synthase; part of a gene cluster that mediates the biosynthesis of a yet unidentified natural product (Probable). The first step in the pathway is performed by Preu6 that condenses 2 acetyl-CoA starter units with 6 malonyl-CoA units to produce lecanoric acid (LA), also known as orsellinate depside, an intermediate that has significant antifungal activity against the plant pathogen Botryosphaeria berengeriana. The biosynthesis probably occurs via the formation of 2 orsellinate intermediates fused together by the C-terminal thioesterase (TE) domain that finally releases lecanoric acid (Probable).","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe NR-PKS, but describe synthesis of different products."
"225","PREU4_PREIS","2151","0.2214","no","Yes","3","More specific","More informative","yes","Non-reducing polyketide synthase; part of a gene cluster that mediates the biosynthesis of a yet unidentified natural product (Probable). The first step in the pathway is performed by Preu4 that produces two major yellow-colored compounds which structures are not stable and hard to be characterized.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both describe NR-PKS, but describe synthesis of different products."
"226","PREU1_PREIS","2388","0.222","no","Yes","4","More specific","More informative","yes","Highly reducing polyketide synthase; part of a gene cluster that mediates the biosynthesis of a yet unidentified natural product.","Highly reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both HR-PKS. Swissprot description states it acts on unidentified natural product, while prediction states specific products."
"227","AOIG_ASPOR","2121","0.2067","no","Yes","3","Equally specific","Equally informative","yes","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of a methylated derivative of known natural products orthosporin and diaporthin. AoiG catalyzes the biosynthesis of the hexaketide isocoumarin scaffold, via condensation of one acetyl-CoA starter unit with 6 malonyl-CoA units. An oxidoreductase that has still to be identified catalyzes the stereospecific reduction of the carbonyl moiety of the hexaketide isocoumarin scaffold to generate the S-configured secondary alcohol at C-11 of orthosporin. The methyltrasferase aoiF then catalyzes the biotransformation of not only orthosporin to diaporthin but also diaporthin to the final product, by performing a tandem methylation of the polyketide core.","Non-reducing polyketide synthase; part of the gene cluster that mediates the biosynthesis of the meroterpenoids arthripenoids. The pathway begins with the HR-PKS atnH that catalyzes two chain-extension steps to form a reduced triketide, which then primes the SAT domain in the NR-PKS atnG to initiate three more cycles of extension to give a linear hexaketide corresponding to the polyketide part of arthripenoids. The FAD-dependent monooxygenase atnJ then performs an oxidative decarboxylation at C11 of the atnH/atnG product","Both NR-PKS, but describe synthesis of different products."
"228","UBP32_MOUSE","1604","0.8768","yes","Yes","3","Equally specific","More informative","yes","Deubiquitinase that can remove conjugated ubiquitin from target proteins, such as RAB7A and LAMTOR1. Acts as a positive regulator of the mTORC1 signaling by mediating deubiquitination of LAMTOR1, thereby promoting the association between LAMTOR1 and the lysosomal V-ATPase complex and subsequent activation of the mTORC1 complex.","Deubiquitinating enzyme involved in the regulation of different processes such as DNA damage response, cell cycle progression, autophagy, ER stress-induced autophagy, innate immune response, and proteasomal degradation. Regulates the proteasomal degradation of p53/TP53, probably by regulating the stability of p53/TP53 protein. Regulates the proteasomal degradation of RNF125, an E3 ubiquitin ligase that mediates K63-linked polyubiquitination of target proteins. Plays a role in ER stress-induced autophagy by regulating the stability of ATG16L1. Acts as a negative regulator of the innate immune response by","Incomplete prediction text?"
"229","MAPKL_TOXGM","1298","0.7011","yes","not-Yes","4","More specific","Equally informative","no","Probable serine/threonine-protein kinase (Probable). Plays a role in coordinated progression of cytokinesis. Restricts duplication of centrosome core structures to one per cycle and ensures proper formation and number of new daughter parasites.","Serine/threonine-protein kinase that phosphorylates 'Ser-2' and 'Ser-5' of the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A and POLR2B. Acts as a negative regulator of transcription by RNA polymerase II. Required for normal embryonic development.","Both describe serine/threonine-protein kinase, but disagree on context/role."
"230","ATG4_TOXGO","3753","0.7736","yes","not-Yes","1","Less specific","Equally informative","no","Putative peptidase that may process C-terminus of ATG8, possibly to regulate ATG8 membrane association. Required for apicoplast biogenesis. Required for maintaining mitochondrial network integrity. Plays a role in intracellular multiplication of tachyzoites.","Essential component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup98-Nup107-160 subcomplex may play a role in stress response.","Swissprot describes a protease, and prediction describes a structural protein."
"231","USP9Y_MOUSE","2556","0.7729","yes","not-Yes","4","More specific","More informative","no","Deubiquitinase that mediates deubiquitination of target proteins. May stabilize target proteins that are important for male germ cell development.","Ubiquitin-protein isopeptidase that specifically cleaves polyubiquitin chains linked through K1 (UBE1), K2 (UBE2A), K21 (UBE2I) and K48 (UBE2L3). Acts as a negative regulator of brassinosteroid (BR) signaling.","Exact same enzyme, but Swissprot describes role in male germ cell development, and prediction describes plant signaling."
"232","LTN1_NEUCR","1600","0.9124","yes","Yes","4","Equally specific","Less informative","no","E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non- stop proteins) and other translation arrest products induced by poly- lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.","E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Ubiquitination leads to polyubiquitination of RPS19 which recruits RPS19 and RPL26 for extraction from the stalled ribosome and subsequent proteasomal degradation.","Describe same pathway, differ in downstream processes."