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README.md
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license: apache-2.0
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tags:
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- biology
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- genomics
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- diffusion
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- single-cell
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- transcriptomics
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# PhenoSeq: Image-Conditioned Diffusion for Single-Cell Transcriptomics
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[](https://huggingface.co/Sentinal4D/PhenoSeq)
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PhenoSeq is a Gaussian diffusion model that **generates scGPT RNA-seq embeddings conditioned on ViT-L microscopy imaging features**. Given fluorescence microscopy images of a cell or well, it predicts a 512-dimensional scGPT embedding representing the transcriptomic state of individual cells — enabling image-to-transcriptome translation at single-cell resolution.
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python train.py --config config.yaml
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```
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Key hyperparameters (see [config.yaml](config.yaml)):
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| Hyperparameter | Value |
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| Batch size | 256 |
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| Learning rate | 1e-4 (cosine with warmup) |
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| Epochs | 5,000 (best at epoch 87) |
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| Model dim | 1,024 |
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| Attention heads | 8 |
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| Cross-attention layers | 6 |
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| Diffusion steps | 1,000 |
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| Schedule | Cosine |
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| EMA decay | 0.9999 |
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## Data
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Training and evaluation data come from the [scGeneScope](https://huggingface.co/datasets/altoslabs/scGeneScope) dataset (Altos Labs):
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## Citation
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If you use
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```bibtex
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@dataset{scgenescope,
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license: apache-2.0
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tags:
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- biology
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- diffusion
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- single-cell
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- transcriptomics
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# PhenoSeq: Image-Conditioned Diffusion for Single-Cell Transcriptomics
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[](https://huggingface.co/Sentinal4D/PhenoSeq)
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[](https://openreview.net/forum?id=ACHMa1e8J1)
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[](https://github.com/reednaidoo/PhenoSeq)
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PhenoSeq is a Gaussian diffusion model that **generates scGPT RNA-seq embeddings conditioned on ViT-L microscopy imaging features**. Given fluorescence microscopy images of a cell or well, it predicts a 512-dimensional scGPT embedding representing the transcriptomic state of individual cells — enabling image-to-transcriptome translation at single-cell resolution.
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python train.py --config config.yaml
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```
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## Data
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Training and evaluation data come from the [scGeneScope](https://huggingface.co/datasets/altoslabs/scGeneScope) dataset (Altos Labs):
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## Citation
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If you use PhenoSeq, please cite our paper:
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```bibtex
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@inproceedings{naidoo2026phenoseq,
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title = {Cell Painting Generates Single-Cell Transcriptomics via Conditional Diffusion},
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author = {Naidoo, Reed and Hu, Jingyu and Tripodi, Giuseppe and Bakal, Chris and Chakraborti, Tapabrata},
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booktitle = {ICML 2026 Workshop on Multi-modal Foundation Models and Large Language Models for Life Sciences (FM4LS)},
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year = {2026},
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url = {https://openreview.net/forum?id=ACHMa1e8J1}
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}
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```
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Please also cite the training dataset:
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```bibtex
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@dataset{scgenescope,
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