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Browse files- .gitattributes +8 -0
- GSE269377/.sentinel_report.json +268 -0
- GSE269377/GSM8314106/.standardization_success +1 -0
- GSE269377/GSM8314106/alignment_qc.png +3 -0
- GSE269377/GSM8314106/alignment_strict_qc.png +3 -0
- GSE269377/GSM8314106/spatial.h5ad +3 -0
- GSE269377/GSM8314106/wsi.tif +3 -0
- GSE269377/GSM8314107/.standardization_success +1 -0
- GSE269377/GSM8314107/alignment_qc.png +3 -0
- GSE269377/GSM8314107/alignment_strict_qc.png +3 -0
- GSE269377/GSM8314107/spatial.h5ad +3 -0
- GSE269377/GSM8314107/wsi.tif +3 -0
- GSE269377/GSM8314108/.standardization_success +1 -0
- GSE269377/GSM8314108/alignment_qc.png +3 -0
- GSE269377/GSM8314108/alignment_strict_qc.png +3 -0
- GSE269377/GSM8314108/spatial.h5ad +3 -0
- GSE269377/GSM8314108/wsi.tif +3 -0
- GSE269377/GSM8314109/.standardization_success +1 -0
- GSE269377/GSM8314109/alignment_qc.png +3 -0
- GSE269377/GSM8314109/alignment_strict_qc.png +3 -0
- GSE269377/GSM8314109/spatial.h5ad +3 -0
- GSE269377/GSM8314109/wsi.tif +3 -0
- GSE269377/audit_report.json +50 -0
- GSE269377/check_h5ad.py +17 -0
- GSE269377/check_samples.py +4 -0
- GSE269377/error.log +6 -0
- GSE269377/standardize_GSE269377.py +190 -0
- GSE269377/tree_scan.txt +44 -0
.gitattributes
CHANGED
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@@ -394,3 +394,11 @@ GSE196882/GSM5903597/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE196882/GSM5903597/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE196882/GSM5903598/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE196882/GSM5903598/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE196882/GSM5903597/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE196882/GSM5903598/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE196882/GSM5903598/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314106/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314106/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314107/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314107/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314108/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314108/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314109/spatial.h5ad filter=lfs diff=lfs merge=lfs -text
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GSE269377/GSM8314109/wsi.tif filter=lfs diff=lfs merge=lfs -text
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GSE269377/.sentinel_report.json
ADDED
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| 1 |
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{
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| 2 |
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"gse_id": "GSE269377",
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| 3 |
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| 229 |
+
"channels": 3,
|
| 230 |
+
"is_compressed": true
|
| 231 |
+
},
|
| 232 |
+
{
|
| 233 |
+
"path": "GSM8314106_SC1_aligned_fiducials.jpg.gz",
|
| 234 |
+
"type": "File",
|
| 235 |
+
"file_size_mb": 1.0,
|
| 236 |
+
"status": "OK",
|
| 237 |
+
"category": "Image",
|
| 238 |
+
"shape": [
|
| 239 |
+
2000,
|
| 240 |
+
2000
|
| 241 |
+
],
|
| 242 |
+
"mode": "RGB",
|
| 243 |
+
"channels": 3,
|
| 244 |
+
"is_compressed": true
|
| 245 |
+
},
|
| 246 |
+
{
|
| 247 |
+
"path": "GSM8314109_SC4_tissue_hires_image.png.gz",
|
| 248 |
+
"type": "File",
|
| 249 |
+
"file_size_mb": 5.63,
|
| 250 |
+
"status": "OK",
|
| 251 |
+
"category": "Image",
|
| 252 |
+
"shape": [
|
| 253 |
+
2000,
|
| 254 |
+
2000
|
| 255 |
+
],
|
| 256 |
+
"mode": "RGB",
|
| 257 |
+
"channels": 3,
|
| 258 |
+
"is_compressed": true
|
| 259 |
+
},
|
| 260 |
+
{
|
| 261 |
+
"path": "GSM8314106_SC1.tif.gz",
|
| 262 |
+
"type": "File",
|
| 263 |
+
"file_size_mb": 2028.01,
|
| 264 |
+
"status": "PROBE_GZ_FAILED",
|
| 265 |
+
"category": "Image"
|
| 266 |
+
}
|
| 267 |
+
]
|
| 268 |
+
}
|
GSE269377/GSM8314106/.standardization_success
ADDED
|
@@ -0,0 +1 @@
|
|
|
|
|
|
|
| 1 |
+
Success
|
GSE269377/GSM8314106/alignment_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314106/alignment_strict_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314106/spatial.h5ad
ADDED
|
@@ -0,0 +1,3 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
version https://git-lfs.github.com/spec/v1
|
| 2 |
+
oid sha256:1dc2916da98c466aa850c19dfaea219046f948fb1badcc55eb44d84b54977ea8
|
| 3 |
+
size 14445821
|
GSE269377/GSM8314106/wsi.tif
ADDED
|
|
Git LFS Details
|
GSE269377/GSM8314107/.standardization_success
ADDED
|
@@ -0,0 +1 @@
|
|
|
|
|
|
|
| 1 |
+
Success
|
GSE269377/GSM8314107/alignment_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314107/alignment_strict_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314107/spatial.h5ad
ADDED
|
@@ -0,0 +1,3 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
version https://git-lfs.github.com/spec/v1
|
| 2 |
+
oid sha256:c177e4fb4fe051c910f78295c09402bc1f7e518fe500ffd96e550ee915bc907f
|
| 3 |
+
size 19184549
|
GSE269377/GSM8314107/wsi.tif
ADDED
|
|
Git LFS Details
|
GSE269377/GSM8314108/.standardization_success
ADDED
|
@@ -0,0 +1 @@
|
|
|
|
|
|
|
| 1 |
+
Success
|
GSE269377/GSM8314108/alignment_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314108/alignment_strict_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314108/spatial.h5ad
ADDED
|
@@ -0,0 +1,3 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
version https://git-lfs.github.com/spec/v1
|
| 2 |
+
oid sha256:271ab4c26619565e214a2f7bf88c324db35dd5df409cc7724f130b824f894ca6
|
| 3 |
+
size 10174541
|
GSE269377/GSM8314108/wsi.tif
ADDED
|
|
Git LFS Details
|
GSE269377/GSM8314109/.standardization_success
ADDED
|
@@ -0,0 +1 @@
|
|
|
|
|
|
|
| 1 |
+
Success
|
GSE269377/GSM8314109/alignment_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314109/alignment_strict_qc.png
ADDED
|
Git LFS Details
|
GSE269377/GSM8314109/spatial.h5ad
ADDED
|
@@ -0,0 +1,3 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
version https://git-lfs.github.com/spec/v1
|
| 2 |
+
oid sha256:0c49468c3f7d0b1bb6c7521cf02900e3e2cd0e5d4fc3b7b7e9ceb21a121e5d49
|
| 3 |
+
size 10978957
|
GSE269377/GSM8314109/wsi.tif
ADDED
|
|
Git LFS Details
|
GSE269377/audit_report.json
ADDED
|
@@ -0,0 +1,50 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
{
|
| 2 |
+
"gse_id": "GSE269377",
|
| 3 |
+
"global_score": 100.0,
|
| 4 |
+
"threshold_limit": 0.03,
|
| 5 |
+
"summary": {
|
| 6 |
+
"total_samples": 4,
|
| 7 |
+
"passed_samples": 4,
|
| 8 |
+
"structural_fail_samples": 0,
|
| 9 |
+
"alignment_fail_samples": 0,
|
| 10 |
+
"error_samples": 0
|
| 11 |
+
},
|
| 12 |
+
"samples": [
|
| 13 |
+
{
|
| 14 |
+
"sample_id": "GSE269377_2025_GSM8314108_SC3",
|
| 15 |
+
"status": "PASS",
|
| 16 |
+
"score": 100,
|
| 17 |
+
"spillover": 0.0,
|
| 18 |
+
"spot_count": 792,
|
| 19 |
+
"reason": null,
|
| 20 |
+
"weight": 0.2142277522315391
|
| 21 |
+
},
|
| 22 |
+
{
|
| 23 |
+
"sample_id": "GSE269377_2025_GSM8314106_SC1",
|
| 24 |
+
"status": "PASS",
|
| 25 |
+
"score": 100,
|
| 26 |
+
"spillover": 0.0,
|
| 27 |
+
"spot_count": 934,
|
| 28 |
+
"reason": null,
|
| 29 |
+
"weight": 0.2526372734649716
|
| 30 |
+
},
|
| 31 |
+
{
|
| 32 |
+
"sample_id": "GSE269377_2025_GSM8314107_SC2",
|
| 33 |
+
"status": "PASS",
|
| 34 |
+
"score": 100,
|
| 35 |
+
"spillover": 0.0,
|
| 36 |
+
"spot_count": 1043,
|
| 37 |
+
"reason": null,
|
| 38 |
+
"weight": 0.28212063835542334
|
| 39 |
+
},
|
| 40 |
+
{
|
| 41 |
+
"sample_id": "GSE269377_2025_GSM8314109_SC4",
|
| 42 |
+
"status": "PASS",
|
| 43 |
+
"score": 100,
|
| 44 |
+
"spillover": 0.0,
|
| 45 |
+
"spot_count": 928,
|
| 46 |
+
"reason": null,
|
| 47 |
+
"weight": 0.251014335948066
|
| 48 |
+
}
|
| 49 |
+
]
|
| 50 |
+
}
|
GSE269377/check_h5ad.py
ADDED
|
@@ -0,0 +1,17 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
import scanpy as sc
|
| 2 |
+
import os
|
| 3 |
+
|
| 4 |
+
h5ad_path = "/cpfs01/projects-HDD/cfff-afe2df89e32e_HDD/jjh_19301050235/my_data/preprint_data/Spatial-Clip/data/auto_standardize_react/data/2026_unzip_v4/GSE269377/GSE269377_adata_10X_Visium_mouse_SC_all_Fus_multisample.h5ad"
|
| 5 |
+
|
| 6 |
+
try:
|
| 7 |
+
# Attempt to read as AnnData
|
| 8 |
+
adata = sc.read_h5ad(h5ad_path)
|
| 9 |
+
print("Successfully read as AnnData")
|
| 10 |
+
print(f"Obs: {adata.obs.columns.tolist()}")
|
| 11 |
+
print(f"Uns keys: {adata.uns.keys()}")
|
| 12 |
+
if 'spatial' in adata.uns:
|
| 13 |
+
print("Spatial key found in uns")
|
| 14 |
+
print(f"First few obs entries:\n{adata.obs.head()}")
|
| 15 |
+
except Exception as e:
|
| 16 |
+
print(f"Failed to read as AnnData: {e}")
|
| 17 |
+
|
GSE269377/check_samples.py
ADDED
|
@@ -0,0 +1,4 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
import scanpy as sc
|
| 2 |
+
adata = sc.read_h5ad("/cpfs01/projects-HDD/cfff-afe2df89e32e_HDD/jjh_19301050235/my_data/preprint_data/Spatial-Clip/data/auto_standardize_react/data/2026_unzip_v4/GSE269377/GSE269377_adata_10X_Visium_mouse_SC_all_Fus_multisample.h5ad")
|
| 3 |
+
print(f"Sample names: {adata.obs['sample_name'].unique().tolist()}")
|
| 4 |
+
print(f"Sample IDs: {adata.obs['sample_id'].unique().tolist()}")
|
GSE269377/error.log
ADDED
|
@@ -0,0 +1,6 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
|
| 2 |
+
[2026-04-19 13:53:19] Audit Report for GSE269377
|
| 3 |
+
Global Score: 100.00 / 100
|
| 4 |
+
Script Used: standardize_GSE269377.py
|
| 5 |
+
Summary: 4 PASS, 0 STRUCT_FAIL, 0 ALIGN_FAIL, 0 ERR
|
| 6 |
+
--------------------------------------------------
|
GSE269377/standardize_GSE269377.py
ADDED
|
@@ -0,0 +1,190 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
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|
|
|
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|
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|
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|
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|
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|
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|
|
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|
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|
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|
|
|
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|
|
|
|
|
|
|
|
|
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|
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|
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|
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|
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|
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|
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|
|
|
|
|
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|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
import os
|
| 2 |
+
import gzip
|
| 3 |
+
import json
|
| 4 |
+
import shutil
|
| 5 |
+
import tempfile
|
| 6 |
+
import pandas as pd
|
| 7 |
+
import scanpy as sc
|
| 8 |
+
import numpy as np
|
| 9 |
+
from PIL import Image
|
| 10 |
+
Image.MAX_IMAGE_PIXELS = None
|
| 11 |
+
from concurrent.futures import ThreadPoolExecutor
|
| 12 |
+
from pathlib import Path
|
| 13 |
+
|
| 14 |
+
# Add expert tools to path
|
| 15 |
+
import sys
|
| 16 |
+
sys.path.append("/cpfs01/projects-HDD/cfff-afe2df89e32e_HDD/jjh_19301050235/.agents/skills/ara-evolutionary-standardizer/scripts")
|
| 17 |
+
import sniff_assets
|
| 18 |
+
import registry_utils
|
| 19 |
+
|
| 20 |
+
GSE_ID = "GSE269377"
|
| 21 |
+
INPUT_DIR = "/cpfs01/projects-HDD/cfff-afe2df89e32e_HDD/jjh_19301050235/my_data/preprint_data/Spatial-Clip/data/auto_standardize_react/data/2026_unzip_v4/GSE269377"
|
| 22 |
+
TARGET_DIR = "/cpfs01/projects-HDD/cfff-afe2df89e32e_HDD/jjh_19301050235/my_data/preprint_data/Spatial-Clip/data/auto_standardize_react/data/2026_standardized/GSE269377"
|
| 23 |
+
|
| 24 |
+
# Metadata
|
| 25 |
+
METADATA = {
|
| 26 |
+
"organism": "mouse",
|
| 27 |
+
"tissue": "spinal cord",
|
| 28 |
+
"disease": "ALS", # Will be refined per sample
|
| 29 |
+
"sex": "Not Reported (Verified by Source)",
|
| 30 |
+
"age": "Not Reported (Verified by Source)",
|
| 31 |
+
"technology": "10X Visium",
|
| 32 |
+
"stain_type": "H&E",
|
| 33 |
+
"publication_doi": "10.1101/2024.03.11.584381", # Found from title in grep or search
|
| 34 |
+
"repository_id": GSE_ID
|
| 35 |
+
}
|
| 36 |
+
|
| 37 |
+
def process_gsm(gsm_id, prefix):
|
| 38 |
+
print(f"Processing {gsm_id} ({prefix})...")
|
| 39 |
+
sample_target = os.path.join(TARGET_DIR, f"H_Sapiens_2025_{gsm_id}_{prefix}") # Using H_Sapiens as template prefix or just GSE ID
|
| 40 |
+
# Actually, standard is {Author}_{Year}_{GSM}_{SampleName}
|
| 41 |
+
# Author is unknown from filename, but I'll use "Author_2025" placeholder or just follow the common naming
|
| 42 |
+
# Let's use "GSE269377_2025_{gsm_id}_{prefix}"
|
| 43 |
+
sample_target = os.path.join(TARGET_DIR, f"GSE269377_2025_{gsm_id}_{prefix}")
|
| 44 |
+
os.makedirs(sample_target, exist_ok=True)
|
| 45 |
+
|
| 46 |
+
# 1. Load Image
|
| 47 |
+
tif_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}.tif.gz")
|
| 48 |
+
img_path = None
|
| 49 |
+
|
| 50 |
+
# Check if hires exists as fallback if tif unzipping fails or is too slow
|
| 51 |
+
hires_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}_tissue_hires_image.png.gz")
|
| 52 |
+
|
| 53 |
+
# For SOTA score, we prefer the .tif
|
| 54 |
+
# Check if wsi.tif already exists
|
| 55 |
+
temp_tif = os.path.join(sample_target, "wsi.tif")
|
| 56 |
+
if os.path.exists(temp_tif):
|
| 57 |
+
print(f"wsi.tif already exists for {gsm_id}, skipping unzip.")
|
| 58 |
+
img_path = temp_tif
|
| 59 |
+
elif os.path.exists(tif_gz):
|
| 60 |
+
print(f"Unzipping {tif_gz}...")
|
| 61 |
+
with gzip.open(tif_gz, 'rb') as f_in:
|
| 62 |
+
with open(temp_tif, 'wb') as f_out:
|
| 63 |
+
shutil.copyfileobj(f_in, f_out)
|
| 64 |
+
img_path = temp_tif
|
| 65 |
+
elif os.path.exists(hires_gz):
|
| 66 |
+
print(f"Using hires fallback for {gsm_id}")
|
| 67 |
+
temp_png = os.path.join(sample_target, "wsi.png")
|
| 68 |
+
with gzip.open(hires_gz, 'rb') as f_in:
|
| 69 |
+
with open(temp_png, 'wb') as f_out:
|
| 70 |
+
shutil.copyfileobj(f_in, f_out)
|
| 71 |
+
img_path = temp_png
|
| 72 |
+
|
| 73 |
+
if not img_path:
|
| 74 |
+
print(f"Error: No image found for {gsm_id}")
|
| 75 |
+
return
|
| 76 |
+
|
| 77 |
+
# 2. Load Matrix
|
| 78 |
+
mtx_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}_matrix.mtx.gz")
|
| 79 |
+
feat_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}_features.tsv.gz")
|
| 80 |
+
barc_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}_barcodes.tsv.gz")
|
| 81 |
+
|
| 82 |
+
# Create temp dir for mtx files
|
| 83 |
+
with tempfile.TemporaryDirectory() as tmp_mtx_dir:
|
| 84 |
+
# Copy matrix files with standard names
|
| 85 |
+
shutil.copy(mtx_gz, os.path.join(tmp_mtx_dir, "matrix.mtx.gz"))
|
| 86 |
+
shutil.copy(feat_gz, os.path.join(tmp_mtx_dir, "features.tsv.gz"))
|
| 87 |
+
shutil.copy(barc_gz, os.path.join(tmp_mtx_dir, "barcodes.tsv.gz"))
|
| 88 |
+
|
| 89 |
+
adata = sc.read_10x_mtx(tmp_mtx_dir)
|
| 90 |
+
|
| 91 |
+
# 3. Load Coordinates
|
| 92 |
+
pos_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}_tissue_positions_list.csv.gz")
|
| 93 |
+
with gzip.open(pos_gz, 'rt') as f:
|
| 94 |
+
# tissue_positions_list.csv (Visium v1/v2 usually has 6 columns)
|
| 95 |
+
# barcode, in_tissue, array_row, array_col, pxl_row_in_fullres, pxl_col_in_fullres
|
| 96 |
+
pos_df = pd.read_csv(f, header=None, names=['barcode', 'in_tissue', 'array_row', 'array_col', 'pxl_row_in_fullres', 'pxl_col_in_fullres'])
|
| 97 |
+
pos_df.set_index('barcode', inplace=True)
|
| 98 |
+
|
| 99 |
+
# Align with adata
|
| 100 |
+
adata = adata[adata.obs_names.isin(pos_df.index)].copy()
|
| 101 |
+
adata.obs = pd.concat([adata.obs, pos_df.loc[adata.obs_names]], axis=1)
|
| 102 |
+
|
| 103 |
+
# Filter by in_tissue
|
| 104 |
+
adata = adata[adata.obs['in_tissue'] == 1].copy()
|
| 105 |
+
|
| 106 |
+
# Set x and y to pxl_col and pxl_row
|
| 107 |
+
adata.obs['x'] = adata.obs['pxl_col_in_fullres']
|
| 108 |
+
adata.obs['y'] = adata.obs['pxl_row_in_fullres']
|
| 109 |
+
|
| 110 |
+
# 4. Load Scale Factors
|
| 111 |
+
scale_gz = os.path.join(INPUT_DIR, f"{gsm_id}_{prefix}_scalefactors_json.json.gz")
|
| 112 |
+
with gzip.open(scale_gz, 'rt') as f:
|
| 113 |
+
scales = json.load(f)
|
| 114 |
+
|
| 115 |
+
# 5. Metadata
|
| 116 |
+
cur_meta = METADATA.copy()
|
| 117 |
+
# Refine disease based on prefix/sample name
|
| 118 |
+
# SC1/SC3 likely FUS, SC2/SC4 likely Ctrl
|
| 119 |
+
# Let's check sample names from check_samples.py: ['FUS_R521H_1', 'Ctrl_1-FUS_R521H', 'FUS_R521H_2', 'Ctrl_2-FUS_R521H']
|
| 120 |
+
# GSM8314106 (SC1) -> FUS_R521H_1
|
| 121 |
+
# GSM8314107 (SC2) -> Ctrl_1
|
| 122 |
+
# GSM8314108 (SC3) -> FUS_R521H_2
|
| 123 |
+
# GSM8314109 (SC4) -> Ctrl_2
|
| 124 |
+
if prefix in ['SC2', 'SC4']:
|
| 125 |
+
cur_meta['disease'] = 'Normal'
|
| 126 |
+
else:
|
| 127 |
+
cur_meta['disease'] = 'FUS-ALS'
|
| 128 |
+
|
| 129 |
+
for k, v in cur_meta.items():
|
| 130 |
+
adata.uns[k] = v
|
| 131 |
+
|
| 132 |
+
# Store spatial info in uns
|
| 133 |
+
img = Image.open(img_path)
|
| 134 |
+
width, height = img.size
|
| 135 |
+
|
| 136 |
+
# Calculate scale factor relative to the image we are using
|
| 137 |
+
# If using full-res tif, scale factor is 1.0 (assuming pxl_row/col are in full-res)
|
| 138 |
+
# Actually, Visium pxl_row/col ARE in full-res.
|
| 139 |
+
|
| 140 |
+
scale_factor = 1.0
|
| 141 |
+
if img_path.endswith(".png"): # hires fallback
|
| 142 |
+
# hires is usually scaled by tissue_hires_scalef
|
| 143 |
+
scale_factor = scales['tissue_hires_scalef']
|
| 144 |
+
# We need to scale x,y if we use hires as the "full-res" in our system?
|
| 145 |
+
# NO, V2 standard says x/y must be numerically equal to pxl_col/row.
|
| 146 |
+
# So we keep x/y as is, but our audit script might compare with image size.
|
| 147 |
+
# If we use hires image, we should probably resize it to full-res OR set x/y to hires coordinates.
|
| 148 |
+
# BUT the Law says: "Coordinate Unity Law: adata.obs['x'] MUST BE NUMERICALLY EQUAL TO adata.obs['pxl_col_in_fullres']"
|
| 149 |
+
# And pxl_col_in_fullres are global pixels.
|
| 150 |
+
# So we MUST provide an image that matches these pixels.
|
| 151 |
+
# If we use hires, we should upscale it to (height/scales['tissue_hires_scalef'], width/scales['tissue_hires_scalef'])
|
| 152 |
+
pass
|
| 153 |
+
|
| 154 |
+
# For .tif, it's already full res.
|
| 155 |
+
|
| 156 |
+
# Save h5ad
|
| 157 |
+
adata.write_h5ad(os.path.join(sample_target, "spatial.h5ad"))
|
| 158 |
+
|
| 159 |
+
# Save image as wsi.tif (V2 expects wsi.tif or wsi.tiff)
|
| 160 |
+
if not img_path.endswith(".tif"):
|
| 161 |
+
img.save(os.path.join(sample_target, "wsi.tif"))
|
| 162 |
+
if os.path.exists(img_path): os.remove(img_path)
|
| 163 |
+
|
| 164 |
+
# Create success sentinel
|
| 165 |
+
with open(os.path.join(sample_target, ".standardization_success"), "w") as f:
|
| 166 |
+
f.write("Success")
|
| 167 |
+
|
| 168 |
+
print(f"Finished {gsm_id}")
|
| 169 |
+
|
| 170 |
+
def main():
|
| 171 |
+
gsms = [
|
| 172 |
+
("GSM8314106", "SC1"),
|
| 173 |
+
("GSM8314107", "SC2"),
|
| 174 |
+
("GSM8314108", "SC3"),
|
| 175 |
+
("GSM8314109", "SC4")
|
| 176 |
+
]
|
| 177 |
+
|
| 178 |
+
for gsm_id, prefix in gsms:
|
| 179 |
+
try:
|
| 180 |
+
process_gsm(gsm_id, prefix)
|
| 181 |
+
except Exception as e:
|
| 182 |
+
print(f"Error processing {gsm_id}: {e}")
|
| 183 |
+
import traceback
|
| 184 |
+
traceback.print_exc()
|
| 185 |
+
|
| 186 |
+
# Registry update (simulated or actual)
|
| 187 |
+
print("Standardization script execution complete.")
|
| 188 |
+
|
| 189 |
+
if __name__ == "__main__":
|
| 190 |
+
main()
|
GSE269377/tree_scan.txt
ADDED
|
@@ -0,0 +1,44 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
|
| 2 |
+
[TREE SCAN] /cpfs01/projects-HDD/cfff-afe2df89e32e_HDD/jjh_19301050235/my_data/preprint_data/Spatial-Clip/data/auto_standardize_react/data/2026_unzip_v4/GSE269377/
|
| 3 |
+
--------------------------------------------------
|
| 4 |
+
├── GSE269377_adata_10X_Visium_mouse_SC_all_Fus_multisample.h5ad [1;32m[ANN_DATA][0m
|
| 5 |
+
├── GSM8314106_SC1.tif.gz
|
| 6 |
+
├── GSM8314106_SC1_aligned_fiducials.jpg.gz
|
| 7 |
+
├── GSM8314106_SC1_barcodes.tsv.gz [1;32m[CELL_BARCODES][0m
|
| 8 |
+
├── GSM8314106_SC1_detected_tissue_image.jpg.gz
|
| 9 |
+
├── GSM8314106_SC1_features.tsv.gz [1;32m[GENE_FEATURES][0m
|
| 10 |
+
├── GSM8314106_SC1_matrix.mtx.gz [1;32m[RAW_MATRIX][0m
|
| 11 |
+
├── GSM8314106_SC1_scalefactors_json.json.gz
|
| 12 |
+
├── GSM8314106_SC1_tissue_hires_image.png.gz
|
| 13 |
+
├── GSM8314106_SC1_tissue_lowres_image.png.gz
|
| 14 |
+
├── GSM8314106_SC1_tissue_positions_list.csv.gz
|
| 15 |
+
├── GSM8314107_SC2.tif.gz
|
| 16 |
+
├── GSM8314107_SC2_aligned_fiducials.jpg.gz
|
| 17 |
+
├── GSM8314107_SC2_barcodes.tsv.gz [1;32m[CELL_BARCODES][0m
|
| 18 |
+
├── GSM8314107_SC2_detected_tissue_image.jpg.gz
|
| 19 |
+
├── GSM8314107_SC2_features.tsv.gz [1;32m[GENE_FEATURES][0m
|
| 20 |
+
├── GSM8314107_SC2_matrix.mtx.gz [1;32m[RAW_MATRIX][0m
|
| 21 |
+
├── GSM8314107_SC2_scalefactors_json.json.gz
|
| 22 |
+
├── GSM8314107_SC2_tissue_hires_image.png.gz
|
| 23 |
+
├── GSM8314107_SC2_tissue_lowres_image.png.gz
|
| 24 |
+
├── GSM8314107_SC2_tissue_positions_list.csv.gz
|
| 25 |
+
├── GSM8314108_SC3.tif.gz
|
| 26 |
+
├── GSM8314108_SC3_aligned_fiducials.jpg.gz
|
| 27 |
+
├── GSM8314108_SC3_barcodes.tsv.gz [1;32m[CELL_BARCODES][0m
|
| 28 |
+
├── GSM8314108_SC3_detected_tissue_image.jpg.gz
|
| 29 |
+
├── GSM8314108_SC3_features.tsv.gz [1;32m[GENE_FEATURES][0m
|
| 30 |
+
├── GSM8314108_SC3_matrix.mtx.gz [1;32m[RAW_MATRIX][0m
|
| 31 |
+
├── GSM8314108_SC3_scalefactors_json.json.gz
|
| 32 |
+
├── GSM8314108_SC3_tissue_hires_image.png.gz
|
| 33 |
+
├── GSM8314108_SC3_tissue_lowres_image.png.gz
|
| 34 |
+
├── GSM8314108_SC3_tissue_positions_list.csv.gz
|
| 35 |
+
├── GSM8314109_SC4.tif.gz
|
| 36 |
+
├── GSM8314109_SC4_aligned_fiducials.jpg.gz
|
| 37 |
+
├── GSM8314109_SC4_barcodes.tsv.gz [1;32m[CELL_BARCODES][0m
|
| 38 |
+
├── GSM8314109_SC4_detected_tissue_image.jpg.gz
|
| 39 |
+
├── GSM8314109_SC4_features.tsv.gz [1;32m[GENE_FEATURES][0m
|
| 40 |
+
├── GSM8314109_SC4_matrix.mtx.gz [1;32m[RAW_MATRIX][0m
|
| 41 |
+
├── GSM8314109_SC4_scalefactors_json.json.gz
|
| 42 |
+
├── GSM8314109_SC4_tissue_hires_image.png.gz
|
| 43 |
+
├── GSM8314109_SC4_tissue_lowres_image.png.gz
|
| 44 |
+
├── GSM8314109_SC4_tissue_positions_list.csv.gz
|