| { | |
| "problem_id": "Exam-01-T7", | |
| "header": "Efficiency of Oxidative Phosphorylation", | |
| "header_images": [], | |
| "subquestions": [ | |
| { | |
| "sub_id": "Exam-01-T7-1", | |
| "tags": [ | |
| "3.2" | |
| ], | |
| "text_open": "A molecular-level hypothesis has been proposed: for every $2e^-$ transferred through the electron-transfer chain, Complex III transports $2HSuc^-$ across the membrane. The opposing concentration gradients that must be overcome in the two transport events are approximately $1:7$ and $1:8$, respectively. Energy losses from other ion-transport processes at Complexes I and IV may be neglected. The inner-side condition is $pH=8$, the outer-side condition is $pH=5$, succinic acid has $pK_{a1,2}=4.2,5.6$, and the temperature is $310\\,\\mathrm{K}$.\nFor this redox reaction, $\\Delta_rG_m^{\\ominus *}\\approx-2.20\\times10^2\\ \\mathrm{kJ\\,mol^{-1}}$; take the magnitude of the available energy as $220\\ \\mathrm{kJ\\,mol^{-1}}$.\nATP synthase can transport only $HSuc^-$ ions on the outer side of the membrane; neither $H_2Suc$ nor $Suc^{2-}$ outside the membrane can pass through ATP synthase. Thus, of the energy stored in $H_2Suc$, $HSuc^-$, and $Suc^{2-}$ during the redox stage, only the fraction associated with external $HSuc^-$ can be utilized. On this basis, calculate $\\eta_{redox}$.", | |
| "text_images": [], | |
| "full_mark": 3, | |
| "rubric": [ | |
| "$\\eta_{redox} \\approx 0.709 \\times [1 - (RT ln 7 + RT ln 8)/220] \\approx 0.676$ (3 points)" | |
| ], | |
| "rubric_images": [] | |
| } | |
| ] | |
| } | |