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R
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# anova.code.r options(echo=F) # Code to do 2-way anova data <- matrix( scan("anova.input", quiet=T), ncol=3, byrow=T, dimnames=list(NULL, c("stack", "class", "spikes"))) Aov <- aov(spikes ~ stack * class, data.frame(data)) cat(round(summary(Aov)[[1]][1:3,5], dig=3), "\n")
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R
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library(cowplot) plot_grid(spontaneous_gi, MPTP_gi, pff_gi,aso_gi, nrow=1, rel_widths = c(1,1,1,1)) spontaneous_gi <- spontaneous_gi + theme(legend.position = "none") aso_gi <- aso_gi + theme(legend.position = "none") spontaneous_gi+theme(legend.position="right")
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R
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mutate_cond <- function(.data, condition, ..., envir = parent.frame()) { # this function is like mutate but only acts on the rows satisfying the condition # condition <- eval(substitute(condition), .data, envir) .data[condition, ] <- .data[condition, ] %>% mutate(...) .data }
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R
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# Setup the directory structure and timing file res_dir <- "results/03.gaussian_kernel" time_file <- "timings.csv" if (!dir.exists(res_dir)) dir.create(res_dir) if (!file.exists(paste0(res_dir, "/", time_file))) { write_lines("Name,Process,Time", paste0(res_dir, "/", time_file)) }
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R
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# Setup the directory structure and timing file res_dir <- "results/01.replicate_daniel" time_file <- "timings.csv" if (!dir.exists(res_dir)) dir.create(res_dir) if (!file.exists(paste0(res_dir, "/", time_file))) { write_lines("Name,Process,Time", paste0(res_dir, "/", time_file)) }
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R
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# Setup the directory structure and timing file res_dir <- "results/05.deep_kernel" time_file <- "timings.csv" if (!dir.exists(res_dir)) dir.create(res_dir) if (!file.exists(paste0(res_dir, "/", time_file))) { readr::write_lines("Name,Process,Time", paste0(res_dir, "/", time_file)) }
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R
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# Setup the directory structure and timing file res_dir <- "results/07.genomic_kernels" time_file <- "timings.csv" if (!dir.exists(res_dir)) dir.create(res_dir) if (!file.exists(paste0(res_dir, "/", time_file))) { readr::write_lines("Name,Process,Time", paste0(res_dir, "/", time_file)) }
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R
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#' miloR #' #' Milo performs single-cell differential abundance testing. Cell states are modelled #' as representative neighbourhoods on a nearest neighbour graph. Hypothesis testing is performed using a #' negative bionomial generalized linear model. #' #' @docType package #' @importFrom Rcpp evalCpp #' @useDynLib mil...
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R
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#' miloR #' #' Milo performs single-cell differential abundance testing. Cell states are modelled #' as representative neighbourhoods on a nearest neighbour graph. Hypothesis testing is performed using a #' negative bionomial generalized linear model. #' #' @aliases miloR #' @return NULL #' @importFrom Rcpp evalCpp #' ...
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R
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# This file is part of the standard setup for testthat. # It is recommended that you do not modify it. # # Where should you do additional test configuration? # Learn more about the roles of various files in: # * https://r-pkgs.org/testing-design.html#sec-tests-files-overview # * https://testthat.r-lib.org/articles/spec...
177c513a89bc8f516fa93d9bad9801901896c61a0bcc817d3d5305c3732d2da3
R
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# Computes a linear kernel for large data matrices # Normalizing by rows avoids an internal copy that may exceed available # vector memory bigLK <- function(X, normalize = TRUE) { K <- tcrossprod(X) if (normalize) { dd <- mean(diag(K)) for (i in 1:nrow(K)) { if (i %% 500 == 0) cat(i, "\r") K...
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R
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#' The miloR package #' #' The \pkg{miloR} package provides modular functions to perform differential #' abundance testing on replicated single-cell experiments. For details please #' see the vignettes \code{vignette("milo_demo", package="miloR")} and #' \code{vignette("milo_gastrulation", package="miloR")}. #' #' @aut...
05366257701506222bc5ab86fd0478f39d86bb703453dac56d09f0f59bb31b7b
R
418
14
library(Seurat) counts <- read.csv('SCP1184/expression/expression_PertCortex.new.txt', sep='\t') metadata <- read.csv('SCP1184/metadata/meta_PertCortex.txt', sep='\t') metadata <- metadata[-1,] counts <- counts[-1,] genenames <- counts[,1] counts <- counts[,-1] rownames(counts) <- genenames data <- CreateSeuratObje...
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R
419
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# Load libraries library(Seurat) library(ggplot2) ## Function for UMAP plot with n = 3 columns and many markers without axes plotMarker <- function(seu_obj, markers){ p <- FeaturePlot(seu_obj, features = markers, reduction = "umap", combine = FALSE, sort.cell = TRUE) for(i in 1:length(p)) { p[[i]] <- p[[i]] + ...
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R
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fisher_test <- function(a, b, bg){ a = unlist(a); b = unlist(b) x = length(intersect(a,b)) m = length(setdiff(a,b)) n = length(setdiff(b,a)) k = length(setdiff(bg, union(a,b))) mat = matrix(c(x,m,n,k),2,2) print(mat) return(fisher.test(mat, alternative="greater")$p.value) } jaccard_index <- ...
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R
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sc_model <- c("Sigmoid" = "#666666", "Tanh" = "#f0027f", "ReLU" = "#7fc97f", "Linear" = "#beaed4", "Gaussian" = "#fdc086", "Arc-cosine" = "#386cb0") sc_data <- c("gPCs" = "#1b9e77", "hPCs" = "#8da0cb", "SNPs" = "#d95...
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R
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# R session information {-} Details on the R version used for making this book. The source code is available at [`LieberInstitute/VisiumLIBD`](https://github.com/LieberInstitute/VisiumLIBD). ```{r session_packages, eval = TRUE, message = FALSE} ## Load the package at the top of your script library("sessioninfo") ``` ...
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R
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## Wire Hang -- wire_hang <- readr::read_csv(here("Analysis_Files", "PFF", "PFF_Wire_Hang - Wire_Hang.csv")) wire_hang <- wire_hang %>% filter(DPI!=90) wire_hang$Genotype <- factor(wire_hang$Genotype,levels=c("WT","HET","MUT")) wire_hang$DPI <- as.character(wire_hang$DPI) wire_hang$DPI <- plyr::revalue(wire_hang$DPI,...
b8f1dc605ffdc9df887721ffdf079e7b3b86d8bce629347effbe6af9d99b7b65
R
530
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uesthreads=33 projHeme1 <- ArchRProject( ArrowFiles = arrow_path, outputDirectory = "Save-projHeme-Raw-V20240710", copyArrows = TRUE, geneAnnotation = geneAnnotation, genomeAnnotation = genomeAnnotation, threads = getArchRThreads() ) projHeme1 #2909835 output_directory=getOutputDirectory(projHe...
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R
538
20
shinyUI( fluidPage( tags$head( tags$link(rel = "stylesheet", type = "text/css", href = "styles.css") ), #shinyjs::useShinyjs(), navbarPage(title = strong("STEPN"), windowTitle = "Multidimensional STEPN", fluid = TRUE, id = "nav", ...
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R
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20
# devtools::test() ## TODO # # if only 2 groups, within-group and between-group filtered intensity columns are the exact same # # make synthetic datasets, then apply filter_dataset() -->> assert filtering is correct # # unit tests with pass and fail assertions to QC the data structures integrity check functions; # # c...
85b8a22c0f28aa6d559893d0c447d026ac1d5edbd5c205617d0f59cc7329eefe
R
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cli::cli_h2("┗ [Vasc-AoP] Setting project configs") #----------------# ####🔺Options #### #----------------# options( verbose = FALSE, scipen = 999L, digits = 4L, na.action = "na.omit", contrasts = c("contr.sum", "contr.poly"), seed = 256, dplyr.summarise.inform = FALSE ) set.seed(getOpti...
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R
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#' Calculate variable genes #' @param x an integer #' @noRd # If no variable genes are provided calc.vargenes = function(se.sc, min.cells){ nonex = which(apply(se.sc@assays$RNA@counts, 1, function(x) length(which(x >0))) < min.cells) # First get rid of non-expressed genes se.sc = subset(se.sc, features = ro...
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R
651
18
source(here::here("src/renv/helpers.R")) # See: ## - https://rstudio.github.io/renv/reference/config.html ## - https://rstudio.github.io/renv/reference/snapshot.html options( repos = c(PPM = "https://packagemanager.posit.co/cran/latest", CRAN = "https://cloud.r-project.org"), renv.config.pak.enabled = FALSE, ...
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R
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# code for main figure 2 panel c # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source...
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R
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# code for main figure 1 panel c # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source...
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R
671
27
setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") library(tidyverse) library(keras3) library(kerastuneR) source("src/weather_tuner_classes.R") tuner <- CVTuner( oracle = BayesianOptimization( objective = Objective("loss", "min"), max_trials = 40 ), hypermodel = HyperModel(), directory = "resul...
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R
679
24
#' sim_discrete #' #' Simulated discrete groups data #' #' Data are simulated single-cells in 4 distinct groups of cells. Cells in each #' group are assigned to 1 of 2 conditions: \emph{A} or \emph{B}. Specifically, #' the cells in block 1 are highly abundant in the \emph{A} condition, whilst #' cells in block 4 are mo...
9fa8e165a365ef719803af86df7e3e09ddd7f5f4507d114caa09a8cc98ca29e9
R
684
23
#' Root mean square error (RMSE) #' #' Calculates the root mean squared error from two vectors of observed #' and expected data. #' Assumes the two vectors are of the same length and that the pairs are #' the same order. #' @param m A vector of expected values #' @param o A vector sof observed values expected to be the...
da8e01038096b4dc60ac483a564c0b8b6adf0f40fd6e0a3e4fffede40377c724
R
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library(GenomicRanges) library(BSgenome.Mfascicularis.NCBI.5.0) library(ArchR) library(parallel) library(tidyverse) geneAnnotation <- readRDS('macaca_geneAnnotation.Rds') seqnames(BSgenome.Mfascicularis.NCBI.5.0)=gsub("MFA","chr",seqnames(BSgenome.Mfascicularis.NCBI.5.0)) genomeAnnotation <- createGenomeAnnotation(g...
54d8d82bae7bf27dfb672d2afd71fa48cb497fa3524719977c77e4f797995760
R
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#' MS-DAP: Mass Spectrometry Downstream Analysis Pipeline for label-free proteomics data. #' #' http://github.com/ftwkoopmans/msdap #' #' @keywords internal #' @import ggplot2 #' @importFrom grDevices colorRampPalette dev.off graphics.off pdf rainbow #' @importFrom graphics abline boxplot legend lines mtext par plot po...
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R
695
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#' Simulated linear trajectory data #' #' Data are simulated single-cells along a single linear trajectory. Cells are #' simulated from 5 groups, and assigned to 1 of 2 conditions; \emph{A} or \emph{B}. #' Data were generated using in the \code{simulate_linear_trajectory} #' function in the \code{dyntoy} package. #' #'...
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R
707
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raw_data <- data.frame( Genotype = paste0("G", 1:18), Group = c(rep("A1", 4), rep("A2", 4), rep("A3", 5), rep("B1", 2), rep("B2", 3)), BestTreatment = c( rep("Q10", 0), rep("R55", 4), # Group A1 rep("Q10", 0), rep("R55", 4), # Group A2 rep("Q10", 4), rep("R55", 1), # Group A3 rep("Q...
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R
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##' Complete copy of nlpca net object ##' @param nlnet a nlnet ##' @return A copy of the input nlnet ##' @author Henning Redestig forkNlpcaNet <- function(nlnet) { res <- new("nlpcaNet") res@net <- nlnet@net res@hierarchic <- nlnet@hierarchic res@fct <- nlnet@fct res@fkt <- nlnet@fkt res@weightDecay <- nlne...
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R
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# Copyright (C) 2009,2010 Bernd Feige # This file is part of avg_q and released under the GPL v3 (see avg_q/COPYING). library(bfown) # Test one-sample t test r<-matrix(runif(100,min=-0.5,max=0.5)+0.1) print(t.test(r)) write.avgq(r,"write_generic a.tmp float32 null_sink - read_generic -c a.tmp 0 1 float32 average -t P...
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R
751
31
#' Print the Epoch Object #' #' @family Epoch methods #' @param object Epoch object #' @return returns an invisible NULL #' @export setMethod("show", "Epoch", function(object) { tbl <- tblData(object) rd <- rowData(object) cd <- colData(object) md <- metaData(object) # -...
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R
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# SAVER combine output ---------------------------------------------------- .combine.saver <- function (saver.list) { est <- do.call(rbind, lapply(saver.list, `[[`, 1)) se <- do.call(rbind, lapply(saver.list, `[[`, 2)) info <- vector("list", 10) names(info) <- c("size.factor", "maxcor", "lambda.max", "lambda....
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R
762
29
df<- read.table("mofa_residuals.tsv", header = TRUE, sep = "\t" ) rownames(df)<-df$ID df<-df[,c(-2)] df$asd_group<-as.numeric(df$asd_group) llm<-lm(asd_group~Factor2,df) y_predicted<-predict(llm,df) y<-df$asd_group sst <- sum((y - mean(y))^2) sse <- sum((y_predicted - y)^2) rsq <- 1 - sse/sst cor(y_...
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R
777
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setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") library(tidyverse) library(tensorflow) tf$config$list_logical_devices() library(keras3) library(kerastuneR) source(textConnection(readLines("09.pc_comparison/11a.weather_tuner.R")[7:103])) tuner <- CVTuner( oracle = BayesianOptimization( objective = Object...
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R
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# code for extended figure 1 panel e # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions so...
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R
808
30
require(keras) build_model <- function(hp) { # The input shape comes from the concatenated outputs of the submodels model <- keras_model_sequential(input_shape = c(15317L)) num_layers <- as.integer(hp[["layers"]]) for (i in 1:num_layers) { model |> layer_dense(units = as.integer(hp[[paste("units_", i)]]...
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R
808
30
require(keras) build_model <- function(hp) { # The input shape comes from the concatenated outputs of the submodels model <- keras_model_sequential(input_shape = c(15503L)) num_layers <- as.integer(hp[["layers"]]) for (i in 1:num_layers) { model |> layer_dense(units = as.integer(hp[[paste("units_", i)]]...
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R
823
27
seeds <- c(424367L, 106463L, 683735L, 486104L, 376453L, 268899L, 673572L, 637487L, 663369L, 301779L) for (s in seeds) { cat("#!/bin/bash", "", "#SBATCH --mem-per-cpu=25G", "#SBATCH --time=24:00:00", "#SBATCH --nodes=1", "#SBATCH --tasks-per-node=16", "", p...
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R
842
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# Optional R template for DCA plotting from exported points suppressPackageStartupMessages({ library(readr) library(ggplot2) }) m <- read_csv(file.path("output","intermediate","DCA_points_model_AD.csv"), show_col_types = FALSE) a <- read_csv(file.path("output","intermediate","DCA_points_treatall_AD.csv"), show_col...
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R
855
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rsqrd <- function(observe, model){ ss_res <- sum((observe-model)^2) y_bar <- mean(observe) ss_tot <- sum((observe-y_bar)^2) return (1-ss_res/ss_tot) } ################################ args <- commandArgs(trailingOnly=T) encoded_file <- args[1] test_index_file <- args[2] model_file <- args[3] outDirection <- a...
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R
860
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library(data.table) library(dplyr) data_dir <- '~/data/scTransform_by_celltype_afterAgg' pos_dir <- '~/UKB_TWAS/WEIGHTS/' ct = 'MonoNC' PB_df = fread(sprintf('%s/Expression_matrices/%s_PB.tsv',data_dir,ct))[,1:4] setwd(sprintf('%s/%s',pos_dir,ct)) t0 = sprintf('ls *scTWAS.wgt.RDat > ../%s_scTWAS.pos',ct) system(t0...
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R
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# R_code.r # Tricky part: divide bins in 8ths, have to combine them here options(echo=F) options(warn=1) # Print them all! # Code to do poisson on/off determination # What p-value to use? (convert percent to fraction) P.value <- scan("macro42_R_input", quiet=T, skip=0, nlines=1) / 100 # How many trials? Trial...
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R
869
31
require(keras) build_model <- function(hp) { # The input shape comes from the concatenated outputs of the submodels model <- keras_model_sequential(input_shape = c(15340L)) num_layers <- as.integer(hp[["layers"]]) for (i in 1:num_layers) { model |> layer_dense(units = as.integer(hp[[paste("units_", i)]]...
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R
869
31
require(keras) build_model <- function(hp) { # The input shape comes from the concatenated outputs of the submodels model <- keras_model_sequential(input_shape = c(15356L)) num_layers <- as.integer(hp[["layers"]]) for (i in 1:num_layers) { model |> layer_dense(units = as.integer(hp[[paste("units_", i)]]...
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R
872
31
require(keras) build_model <- function(hp) { # The input shape comes from the concatenated outputs of the submodels model <- keras_model_sequential(input_shape = c(15384L)) num_layers <- as.integer(hp[["layers"]]) for (i in 1:num_layers) { model |> layer_dense(units = as.integer(hp[[paste("units_", i)]]...
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R
872
31
require(keras) build_model <- function(hp) { # The input shape comes from the concatenated outputs of the submodels model <- keras_model_sequential(input_shape = c(15498L)) num_layers <- as.integer(hp[["layers"]]) for (i in 1:num_layers) { model |> layer_dense(units = as.integer(hp[[paste("units_", i)]]...
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R
875
30
models <- 1:4 folds <- 1:10 for (m in models) { for (k in folds) { cat("#!/bin/bash", "", "#SBATCH --mem-per-cpu=25G", "#SBATCH --time=48:00:00", "#SBATCH --nodes=1", "#SBATCH --tasks-per-node=16", "", paste0("#SBATCH --output=tune_weather_", m, "_",...
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R
884
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library(Seurat) library(tidyverse) library(glue) library(readxl) base_dir <- "/n/scratch/users/s/sad167/EPN/scRNAseq" resources_dir <- file.path(base_dir, 'scripts/resources') source(file.path(resources_dir, 'single_cell_preprocessing_helper_functions.R')) ## Dealing with counts for each sample metadata <- read_exc...
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R
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library(Seurat) library(tidyverse) library(glue) library(readxl) base_dir <- "/n/scratch/users/s/sad167/EPN/scRNAseq" resources_dir <- file.path(base_dir, 'scripts/resources') source(file.path(resources_dir, 'single_cell_preprocessing_helper_functions.R')) ## Dealing with counts for each sample metadata <- read_exc...
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R
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library(sjPlot) library(broom.mixed) # for tidy() library(dplyr) library(emmeans) library(lme4) library(lmerTest) library(ggplot2) library(moments) library(ggeffects) library(robustlmm) library(glmmTMB) library(patchwork) library(forcats) library(scales) df <- read.csv("E:\\work\\q_backup_06_03_2025\\projects\\i...
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R
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library(rio) library(sjPlot) library(emmeans) library(lme4) df <- import("E:\\work\\q_backup_06_03_2025\\projects\\isttc\\results\\allen_mice\\dataset\\cut_30min\\summary_taus_plot_5methods_100_50_20_1_resampling_df_long.csv") df$method <- factor(df$method, levels = c("isttc_full", "acf_full", "pearsonr", "sttc_avg", ...
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setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") library(tidyverse) library(keras3) ### Data preparation ---------------------------------------------------------- d <- read_rds("09.pc_comparison/input_data.rds") x_test <- d$Test$X y_test <- d$Test$y rm(d); gc() # Function for rmse rmse <- function(y, yhat) ...
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##' Print a brief description of nniRes model ##' @title Print a nniRes model ##' @param x An \code{nniRes} object ##' @param ... Not used ##' @return Nothing, used for side-effect ##' @export ##' @author Henning Redestig showNniRes <- function(x, ...) { summary(x) cat(dim(x)["nVar"], "\tVariables\n") cat(dim(x)[...
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R
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setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") library(tidyverse) library(keras3) library(kerastuneR) source("src/weather_tuner_classes.R") cv_y <- read_rds("results/DNNs/cv_data/cv_y.rds") ### swap `overwrite = TRUE` to restart tuning from scratch ### `overwrite = FALSE` picks up from the existing informati...
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R
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rule intersect_mergepeak: input: f"{outd_sum_mergepeak}/mba.whole.union.peak.bed" output: touch(f"{fdir}/{{cl}}_unionpeak.done") threads: 1 resources: walltime = 1, queue = "glean", mail = "a", email = "debug.pie@gmail.com" conda: "sa22" ...
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R
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library(Seurat) library(tidyverse) library(harmony) library(patchwork) #subset E11 matching TW E11 data cds <- readRDS("data/cds.rds") Idents(cds) <- "cell_type" dir.create("tables/E10_E15_majorcellTypes_stages") for (i in levels(cds)) { seu_temp <- subset(cds, idents = i) Idents(seu_temp) <- "stage" sig_genes <...
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R
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source("library.R") library(parallel) if(!file.exists("paths.txt")) { stop("Please make a file called paths.txt with the format: root,cache path_to_root,path_to_cache") } paths <- read.table("paths.txt", header = TRUE, sep = ",") ROOT <- as.character(paths$root) ethoscope_cache <- as.character(paths$cache) BAT...
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R
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##' Creates a large matrix B consisting of an M-by-N tiling of copies ##' of A ##' @title Replicate and tile an array. ##' @param mat numeric matrix ##' @param M number of copies in vertical direction ##' @param N number of copies in horizontal direction ##' @return Matrix consiting of M-by-N tiling copies of input mat...
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R
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# code for extended figure 1 panel c # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions so...
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R
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#' @import TableContainer #' @import osfr #' @import methods #' @importFrom ramify pprint #' @importFrom glue glue #' @importFrom jsonlite fromJSON #' @importFrom stats sd #' @importFrom rlang .data #' @importFrom ggplot2 ggplot geom_line aes labs scale_y_continuous theme element_text #' @importFrom ggtext element_mar...
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R
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# code for extended figure 6 panel b # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions so...
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#--------------# ####🔺infix #### #--------------# '%notin%' <- Negate('%in%') `%ni%` <- `%notin%` # Alias '%||%' <- function(x, y) if (is.null(x)) y else x #-------------# ####🔺here #### #-------------# if (!"here" %in% as.data.frame(installed.packages())$Package) { renv::install("here", prompt = FALSE) } #--...
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R
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library(rio) library(dabestr) library(ggplot2) df <- import("E:\\work\\q_backup_06_03_2025\\projects\\isttc\\results\\allen_mice\\dataset\\cut_30min\\summary_taus_plot_5methods_100_resampling_df_long.csv") df$method <- factor(df$method, levels = c("acf_full", "isttc_full", "sttc_avg", "sttc_concat", "pearsonr")) dabe...
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R
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### Jaccard similarity ### define groups based on sleep features: behA <- c("Gdh", "Lrrk","VAC14", "vham89", "eIF4G", "Omi", "Vps13", "Vps35", "Synj", "Tango14", "Dj1aDj1b", "iPLA2VIA", "Rab39","GBA", "Punch", "Rme8") behB <- c("CHCHD2", "Pink1", "Auxillin", "nutcracker", "anne", "Coq2", "Loqs", "park" ) ### tr...
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R
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# code for extended figure 4 # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source('~/...
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require(keras) build_model <- function(hp) { num_layers <- as.integer(hp["layers"]) model <- keras_model_sequential() for (i in 1:num_layers) { if (i == 1) { model |> layer_dense(units = hp[paste0("units_", i)], activation = "tanh", kernel_i...
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R
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require(keras) build_model <- function(hp) { num_layers <- as.integer(hp["layers"]) model <- keras_model_sequential() for (i in 1:num_layers) { if (i == 1) { model |> layer_dense(units = hp[paste0("units_", i)], activation = "sigmoid", kerne...
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R
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plot.fftfilter<-function(infile,add=F,...) { fftsize<-NULL freq.resolution<-NULL factors<-NULL while (TRUE) { line <- readLines(infile, n = 1, ok = TRUE) if (length(line)==0 || line == "End of script") break #print(line) r<-sub('^fftfilter: FFT size ([0-9]+) points, Frequency resolution ([.0-9]+)Hz$','\\1 \...
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R
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separate_mfg_sfg <- function(df) { # load csv with mfg, sfg electrodes regions_df <- read_csv(path(here(), "munge", "mni_coordinates_all_subs_with_detailed_regions.csv")) # create mfg df mfg_df <- regions_df %>% filter(region == "mfg") %>% mutate(elec_id = paste0(subject, "_", Electrode)) # cre...
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R
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# code for extended figure 4 # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source('~/...
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R
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#' Create matrix of cellular composition profiles consisting of proportion #' of noise and remaining compostion in the ratio of supplied cell types #' #' @param cellProp A vector of the required cellular proportions. Must be #' named with cell labels. #' @param noise A vector of the proportion of noise. #' @return A ...
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# code for main figure 3 panel d # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source...
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R
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library(Seurat) library(tidyverse) library(harmony) library(patchwork) #loading E15 mouse and spatial data cds <- readRDS("datacds.rds") cds1 <- readRDS("/Users/naghamkhourifarah/Downloads/GSE245469_RAW/data/combined_HQ.rds") cds2 <- subset(cds,cells = rownames(cds@meta.data[cds$stage == "E15",])) Idents(cds2) <- cds2$...
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R
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#!/usr/bin/env Rscript source("plot_fftfilter.R") plot.fftfilter.boundaries<-function(sfreq,boundaries) { script<-paste(" null_source", sfreq, "1 1 1s 1s fftfilter -V", boundaries," null_sink ") library(bfown) a <- open.avgq("avg_q_vogl") cat(script,"\n-\n!echo -F stdout End of script\\n\nnull_sink\n-\n", ...
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R
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library(Biostrings) library(GenomicRanges) # 1. 读取基因组 (示例:第一条染色体) pf_genome <- readDNAStringSet("./PlasmoDB-9.0_Pfalciparum3D7_Genome.fasta") names(pf_genome) chr1_seq <- pf_genome[[1]] # 获取DNA序列 chr1_seq <- pf_genome[[14]] chr1_seq <- pf_genome[[7]] # 2. 定义计算函数 (滑动窗口) calculate_cpg_density <- function(dna_seq, wind...
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R
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# code for extended figure 4 # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source('~/...
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R
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# code for extended figure 4 # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source('~/...
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install.packages('e1071') #if (!requireNamespace("BiocManager", quietly = TRUE)) # install.packages("BiocManager") BiocManager::install("preprocessCore") inputFile="merge.normalize.txt" #ÊäÈëÎļþ setwd("C:\\Users\\wx197\\Desktop\\×ÔÑ§Íø113»úÆ÷ѧϰ\\205.geoML×ÊÁÏ\\205.geoML×ÊÁÏ\\24.CIBERSORT") #É...
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# code for main figure 3 panel c # source the functions from FLXenium folder, which loads useful libraries and has # a bunch of functions useful for general analysis of spatial data miceadds::source.all('~/Multidimensional_STEPN/4.Xenium/resources/FLXenium/') # source the ependymoma project specific functions source...
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# setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") setwd("/homes/amkusmec/kernels_in_GP/") library(tidyverse) library(argparse) source("src/BGGE2.R") parser <- ArgumentParser() parser$add_argument("--seed", "-s", type = "integer") args <- parser$parse_args() # The phenotypes have already been scaled and center...
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R
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# R_code43.r # Fit a von Mises to synthesized data; compare to a flat line; show fits options(echo=F) options(warn=1) # Print them all! data <- matrix(scan("macro43_R_input", quiet=T), ncol=2, byrow=T) angle = data[,1] rate = data[,2] options(show.error.messages = F) fit0 = try(nls( formula = rate ~ base + p...
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# setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") setwd("/homes/amkusmec/kernels_in_GP/") library(tidyverse) library(argparse) source("src/BGGE2.R") parser <- ArgumentParser() parser$add_argument("--seed", "-s", type = "integer") args <- parser$parse_args() # The phenotypes have already been scaled and center...
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testthat::context("normalization reproducibility") msdap::enable_log(FALSE) datasets = prepare_test_datasets(5000) datasets_as_matrix = datasets$as_matrix for(lbl in names(datasets_as_matrix)) { m_group_id = datasets_as_matrix[[lbl]]$groupid m_log2 = datasets_as_matrix[[lbl]]$mat m_log2[!is.finite(m_log2)] = NA ...
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# setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") setwd("/homes/amkusmec/kernels_in_GP/") library(tidyverse) library(argparse) source("src/BGGE2.R") parser <- ArgumentParser() parser$add_argument("--seed", "-s", type = "integer") args <- parser$parse_args() # The phenotypes have already been scaled and center...
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R
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#' A pre-computed scWGCNA list calculated from the Mouse limb data. #' #' A scWGCNA data list containing the different modules, statistics and calculations #' #' @format A scWGCNA list object, used as example #' @source This package "MmLimbE155.scWGCNA" #' A pre-analyzed, sub-sampled Seurat object, of single cell RNA-...
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#' sim_family #' #' Simulated counts data from a series of simulated family trees #' #' Data are simulated counts from 30 families and includes X and Z design matrices, #' as well as a single large kinship matrix. Kinships between family members are #' dictated by the simulated family, i.e. sibs=0.5, parent-sib=0.5, si...
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#' Benchmarking: Calculate of the AUC of different cases #' #' This function calculates the AUC of different cases #' #' @param out_list The list of prediction probability matrix of each case #' @param cluster The ground-truth #' @param cell_types Character vector of cell types of the data #' #' @import pROC #' @import...
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# These functions do not have any meaningful implementation, just wrapping #' Wrapper functions for calling TableContainer methods #' #' @param x An Epoch object #' #' @rdname Epoch-method #' @examples #' #' # Create an Epoch object #' epoch_data <- matrix(rnorm(1000), nrow = 10) #' rownames(epoch_data) <- paste0("...
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R
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#' This function plots the importance of the features #' #' This function plots the n top importance features decreasing or increasing. #' #' @param data The output of `ds_feature_importance`. #' @param n The number of features to be plotted (Default=30) #' @param Decrease If the importance are plotted decreasing or in...
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# ReadAllNear.r # In case you have read in a different dataNearBase! # Set basic/default parameters # ######################## if (NEAR==0) return cat("Reading in standard dataNearBase for 'Near'\n") a = b = NULL if (MONK=="zen" || MONK=="both") { a = read.table("/data/coord/zen/zenunits", header=T,c...
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# estimateUnwantedVariation ----------------------------------------------- # estimateCellType -------------------------------------------------------- estimateCellType <- function(countData, readData = NULL, annotData = NULL, sp...
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# setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") setwd("/homes/amkusmec/kernels_in_GP/") library(tidyverse) library(argparse) source("src/BGGE2.R") parser <- ArgumentParser() parser$add_argument("--seed", "-s", type = "integer") args <- parser$parse_args() # The phenotypes have already been scaled and center...
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library(dplyr) library(Seurat) library(ggplot2) ## Style plots # Barplot function theme_ggplot = theme(legend.position = "none", plot.title = element_text(hjust=0.5, face="bold"), panel.border = element_blank(), plot.background = element_blank(), panel.background = element_blank(), axis....
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# setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") setwd("/homes/amkusmec/kernels_in_GP/") library(tidyverse) library(argparse) source("src/BGGE2.R") parser <- ArgumentParser() parser$add_argument("--seed", "-s", type = "integer") args <- parser$parse_args() # The phenotypes have already been scaled and center...
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# setwd("~/Documents/yu_lab/kernels_in_GP/Kick_data/") setwd("/homes/amkusmec/kernels_in_GP/") library(tidyverse) library(argparse) source("src/BGGE2.R") parser <- ArgumentParser() parser$add_argument("--seed", "-s", type = "integer") args <- parser$parse_args() # The phenotypes have already been scaled and center...