| {"record_kind": "run_header", "run_id": "pearl-manuscript-s6-20260916", "seq": 1, "trace_sequence": 1, "timestamp": "2026-09-16T10:24:12+00:00", "logged_at": "2026-09-16T10:24:12.350000+00:00", "source_agent": null, "scenario_id": "S6", "provenance": "manuscript-aligned-case-log-v1", "not_wet_lab": false, "world_contract": "manuscript-case-S6", "config": {"communication_mode": "typed_events", "ir_schema_version": 4}, "user_command": "Autonomously characterize the HRP–TMB assay: identify its linear working range and estimate Km and kcat from time-resolved measurements.", "sources": [{"well": "A1", "label": "Enzyme stock (HRP), 3 nM nominal", "volume_ul": 2000, "concentration_nM": 3.0, "concentration_ng_ml": 132.0, "mw_g_per_mol": 44000.0}, {"well": "A2", "label": "Chromogenic substrate stock (TMB), 5.0 mM", "volume_ul": 5000, "concentration_mM": 5.0}, {"well": "A3", "label": "PBS, 1×", "volume_ul": 15000}], "constraints": ["The instruction does not prescribe well layout, an acid stop, or a dilution factor.", "Nominal enzyme stock is 3 nM and 2000 uL.", "96-well plate. Each reaction is read in the same well."], "instrument": {"wavelength_nm": 652, "epsilon_per_M_cm": 39000.0, "path_cm": 0.56, "path_basis": "Applied path is 0.28 cm times the well volume divided by 100 uL. At 200 uL that path is 0.56 cm.", "hrp_mw_g_per_mol": 44000.0, "initial_rate_elapsed_s": [3.0, 6.0, 9.0], "max_substrate_conversion": 0.15, "min_initial_rate_points": 3}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 2, "trace_sequence": 2, "timestamp": "2026-09-16T10:24:24+00:00", "logged_at": "2026-09-16T10:24:24.350000+00:00", "source_agent": "planner", "event_id": "plan-plan-s6", "event_type": "plan", "plan_id": "plan-s6", "revision_id": "rev_0", "rationale": "Dispense a 21-well triplicate substrate series at 10 ng/mL HRP, plus the HRP titration. The 0.5 ng/mL point comes from a diluted aliquot in H12 so that transfer is at least 1 uL. Read each well at 3, 6, and 9 s after TMB is dispensed, within 15% substrate conversion. 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{"action_id": "plan-s6-E7-buffer", "tool_name": "transfer", "source": "reservoir:A3", "dest": "plate:E7", "volume_ul": 136.0, "reagent": "buffer"}, {"action_id": "plan-s6-E7-tmb", "tool_name": "transfer", "source": "reservoir:A2", "dest": "plate:E7", "volume_ul": 64.0, "reagent": "tmb"}, {"action_id": "plan-s6-E7-mix", "tool_name": "mix_well", "well": "E7"}]} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 3, "trace_sequence": 3, "timestamp": "2026-09-16T10:24:36+00:00", "logged_at": "2026-09-16T10:24:36.350000+00:00", "source_agent": null, "event_id": "appr-appr-plan-s6", "event_type": "proceed_signal", "plan_id": "plan-s6", "revision_id": "rev_0", "approval_id": "appr-plan-s6", "approved_action_ids": ["plan-s6-H12-hrp", "plan-s6-H12-buffer", "plan-s6-H12-mix", "plan-s6-A1-hrp", "plan-s6-A1-buffer", "plan-s6-A1-tmb", "plan-s6-A1-mix", "plan-s6-A2-hrp", "plan-s6-A2-buffer", "plan-s6-A2-tmb", "plan-s6-A2-mix", "plan-s6-A3-hrp", "plan-s6-A3-buffer", "plan-s6-A3-tmb", "plan-s6-A3-mix", "plan-s6-A4-hrp", "plan-s6-A4-buffer", "plan-s6-A4-tmb", "plan-s6-A4-mix", "plan-s6-A5-hrp", "plan-s6-A5-buffer", "plan-s6-A5-tmb", "plan-s6-A5-mix", "plan-s6-A6-hrp", "plan-s6-A6-buffer", "plan-s6-A6-tmb", "plan-s6-A6-mix", "plan-s6-A7-hrp", "plan-s6-A7-buffer", "plan-s6-A7-tmb", "plan-s6-A7-mix", "plan-s6-A8-hrp", "plan-s6-A8-buffer", "plan-s6-A8-tmb", "plan-s6-A8-mix", "plan-s6-B1-hrp", "plan-s6-B1-buffer", "plan-s6-B1-tmb", "plan-s6-B1-mix", "plan-s6-C1-hrp", "plan-s6-C1-buffer", "plan-s6-C1-tmb", "plan-s6-C1-mix", "plan-s6-D1-hrp", "plan-s6-D1-buffer", "plan-s6-D1-tmb", "plan-s6-D1-mix", "plan-s6-B2-hrp", "plan-s6-B2-buffer", "plan-s6-B2-tmb", "plan-s6-B2-mix", "plan-s6-C2-hrp", "plan-s6-C2-buffer", "plan-s6-C2-tmb", "plan-s6-C2-mix", "plan-s6-D2-hrp", "plan-s6-D2-buffer", "plan-s6-D2-tmb", "plan-s6-D2-mix", "plan-s6-B3-hrp", "plan-s6-B3-buffer", "plan-s6-B3-tmb", "plan-s6-B3-mix", "plan-s6-C3-hrp", "plan-s6-C3-buffer", "plan-s6-C3-tmb", "plan-s6-C3-mix", "plan-s6-D3-hrp", "plan-s6-D3-buffer", "plan-s6-D3-tmb", "plan-s6-D3-mix", "plan-s6-B4-hrp", "plan-s6-B4-buffer", "plan-s6-B4-tmb", "plan-s6-B4-mix", "plan-s6-C4-hrp", "plan-s6-C4-buffer", "plan-s6-C4-tmb", "plan-s6-C4-mix", "plan-s6-D4-hrp", "plan-s6-D4-buffer", "plan-s6-D4-tmb", "plan-s6-D4-mix", "plan-s6-B5-hrp", "plan-s6-B5-buffer", "plan-s6-B5-tmb", "plan-s6-B5-mix", "plan-s6-C5-hrp", "plan-s6-C5-buffer", "plan-s6-C5-tmb", "plan-s6-C5-mix", "plan-s6-D5-hrp", "plan-s6-D5-buffer", "plan-s6-D5-tmb", "plan-s6-D5-mix", "plan-s6-B6-hrp", "plan-s6-B6-buffer", "plan-s6-B6-tmb", "plan-s6-B6-mix", "plan-s6-C6-hrp", "plan-s6-C6-buffer", "plan-s6-C6-tmb", "plan-s6-C6-mix", "plan-s6-D6-hrp", "plan-s6-D6-buffer", "plan-s6-D6-tmb", "plan-s6-D6-mix", "plan-s6-B7-hrp", "plan-s6-B7-buffer", "plan-s6-B7-tmb", "plan-s6-B7-mix", "plan-s6-C7-hrp", "plan-s6-C7-buffer", "plan-s6-C7-tmb", "plan-s6-C7-mix", "plan-s6-D7-hrp", "plan-s6-D7-buffer", "plan-s6-D7-tmb", "plan-s6-D7-mix", "plan-s6-E1-buffer", "plan-s6-E1-tmb", "plan-s6-E1-mix", "plan-s6-E2-buffer", "plan-s6-E2-tmb", "plan-s6-E2-mix", "plan-s6-E3-buffer", "plan-s6-E3-tmb", "plan-s6-E3-mix", "plan-s6-E4-buffer", "plan-s6-E4-tmb", "plan-s6-E4-mix", "plan-s6-E5-buffer", "plan-s6-E5-tmb", "plan-s6-E5-mix", "plan-s6-E6-buffer", "plan-s6-E6-tmb", "plan-s6-E6-mix", "plan-s6-E7-buffer", "plan-s6-E7-tmb", "plan-s6-E7-mix"], "message": "Approved against the public deck and the stated instruction."} | |
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| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 6, "trace_sequence": 6, "timestamp": "2026-09-16T10:24:53+00:00", "logged_at": "2026-09-16T10:24:53.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-H12-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-H12-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-H12-mix", "action_params": {"well": "H12"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:H12"}}, "result_observation_ids": ["obs-plan-s6-H12-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 7, "trace_sequence": 7, "timestamp": "2026-09-16T10:25:08+00:00", "logged_at": "2026-09-16T10:25:08.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A1-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A1-hrp", "action_params": {"source": "H12", "dest": "A1", "volume_ul": 1.5151515151515151, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "volume_ul": 1.5151515151515151, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A1-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 8, "trace_sequence": 8, "timestamp": "2026-09-16T10:25:14+00:00", "logged_at": "2026-09-16T10:25:14.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A1-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A1-buffer", "action_params": {"source": "A3", "dest": "A1", "volume_ul": 190.4848484848485, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "volume_ul": 190.4848484848485, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A1-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 9, "trace_sequence": 9, "timestamp": "2026-09-16T10:25:23+00:00", "logged_at": "2026-09-16T10:25:23.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A1-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A1-tmb", "action_params": {"source": "A2", "dest": "A1", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A1-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 10, "trace_sequence": 10, "timestamp": "2026-09-16T10:25:25+00:00", "logged_at": "2026-09-16T10:25:25.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A1-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A1-mix", "action_params": {"well": "A1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A1", "enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "label_implied_enzyme_nM": 0.011363636363636362, "label_implied_enzyme_ng_ml": 0.5, "rate_A652_per_min": 0.03948589383770591, "rate_uM_per_s": 0.03013270286760219, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 2, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A1-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 11, "trace_sequence": 11, "timestamp": "2026-09-16T10:25:26+00:00", "logged_at": "2026-09-16T10:25:26.350000+00:00", "source_agent": null, "event_id": "obs-s6-A1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A1-t3", "wells": {"plate:A1": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "label_implied_enzyme_nM": 0.011363636363636362, "label_implied_enzyme_ng_ml": 0.5, "rate_A652_per_min": 0.03948589383770591, "rate_uM_per_s": 0.03013270286760219, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 2, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.0019742946918852957, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:25:23+00:00", "acquired_at": "2026-09-16T10:25:26+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0004519905430140328}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 12, "trace_sequence": 12, "timestamp": "2026-09-16T10:25:29+00:00", "logged_at": "2026-09-16T10:25:29.350000+00:00", "source_agent": null, "event_id": "obs-s6-A1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A1-t6", "wells": {"plate:A1": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "label_implied_enzyme_nM": 0.011363636363636362, "label_implied_enzyme_ng_ml": 0.5, "rate_A652_per_min": 0.03948589383770591, "rate_uM_per_s": 0.03013270286760219, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 2, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.0039485893837705914, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:25:23+00:00", "acquired_at": "2026-09-16T10:25:29+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0009039810860280656}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 13, "trace_sequence": 13, "timestamp": "2026-09-16T10:25:32+00:00", "logged_at": "2026-09-16T10:25:32.350000+00:00", "source_agent": null, "event_id": "obs-s6-A1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A1-t9", "wells": {"plate:A1": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.011363636363636362, "enzyme_concentration_ng_ml": 0.5, "enzyme_concentration_M": 1.1363636363636362e-11, "label_implied_enzyme_nM": 0.011363636363636362, "label_implied_enzyme_ng_ml": 0.5, "rate_A652_per_min": 0.03948589383770591, "rate_uM_per_s": 0.03013270286760219, "hrp_source": "H12", "hrp_source_labware": "plate", "dilution_factor": 2, "neat_enzyme_ul": 0.7575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.005922884075655887, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:25:23+00:00", "acquired_at": "2026-09-16T10:25:32+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0013559716290420984}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 14, "trace_sequence": 14, "timestamp": "2026-09-16T10:25:44+00:00", "logged_at": "2026-09-16T10:25:44.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A2-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A2-hrp", "action_params": {"source": "A1", "dest": "A2", "volume_ul": 1.5151515151515151, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "volume_ul": 1.5151515151515151, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A2-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 15, "trace_sequence": 15, "timestamp": "2026-09-16T10:25:50+00:00", "logged_at": "2026-09-16T10:25:50.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A2-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A2-buffer", "action_params": {"source": "A3", "dest": "A2", "volume_ul": 190.4848484848485, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "volume_ul": 190.4848484848485, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A2-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 16, "trace_sequence": 16, "timestamp": "2026-09-16T10:25:54+00:00", "logged_at": "2026-09-16T10:25:54.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A2-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A2-tmb", "action_params": {"source": "A2", "dest": "A2", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A2-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 17, "trace_sequence": 17, "timestamp": "2026-09-16T10:25:55+00:00", "logged_at": "2026-09-16T10:25:55.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A2-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A2-mix", "action_params": {"well": "A2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A2", "enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07897178767541183, "rate_uM_per_s": 0.06026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A2-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 18, "trace_sequence": 18, "timestamp": "2026-09-16T10:25:57+00:00", "logged_at": "2026-09-16T10:25:57.350000+00:00", "source_agent": null, "event_id": "obs-s6-A2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A2-t3", "wells": {"plate:A2": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07897178767541183, "rate_uM_per_s": 0.06026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.0039485893837705914, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:25:54+00:00", "acquired_at": "2026-09-16T10:25:57+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0009039810860280656}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 19, "trace_sequence": 19, "timestamp": "2026-09-16T10:26:00+00:00", "logged_at": "2026-09-16T10:26:00.350000+00:00", "source_agent": null, "event_id": "obs-s6-A2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A2-t6", "wells": {"plate:A2": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07897178767541183, "rate_uM_per_s": 0.06026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.007897178767541183, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:25:54+00:00", "acquired_at": "2026-09-16T10:26:00+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0018079621720561313}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 20, "trace_sequence": 20, "timestamp": "2026-09-16T10:26:03+00:00", "logged_at": "2026-09-16T10:26:03.350000+00:00", "source_agent": null, "event_id": "obs-s6-A2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A2-t9", "wells": {"plate:A2": {"enzyme_volume_ul": 1.5151515151515151, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.022727272727272724, "enzyme_concentration_ng_ml": 1.0, "enzyme_concentration_M": 2.2727272727272724e-11, "rate_A652_per_min": 0.07897178767541183, "rate_uM_per_s": 0.06026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 1.5151515151515151, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.011845768151311773, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:25:54+00:00", "acquired_at": "2026-09-16T10:26:03+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.002711943258084197}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 21, "trace_sequence": 21, "timestamp": "2026-09-16T10:26:16+00:00", "logged_at": "2026-09-16T10:26:16.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A3-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A3-hrp", "action_params": {"source": "A1", "dest": "A3", "volume_ul": 3.0303030303030303, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "volume_ul": 3.0303030303030303, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A3-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 22, "trace_sequence": 22, "timestamp": "2026-09-16T10:26:24+00:00", "logged_at": "2026-09-16T10:26:24.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A3-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A3-buffer", "action_params": {"source": "A3", "dest": "A3", "volume_ul": 188.96969696969697, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "volume_ul": 188.96969696969697, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A3-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 23, "trace_sequence": 23, "timestamp": "2026-09-16T10:26:28+00:00", "logged_at": "2026-09-16T10:26:28.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A3-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A3-tmb", "action_params": {"source": "A2", "dest": "A3", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A3-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 24, "trace_sequence": 24, "timestamp": "2026-09-16T10:26:29+00:00", "logged_at": "2026-09-16T10:26:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A3-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A3-mix", "action_params": {"well": "A3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A3", "enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15794357535082365, "rate_uM_per_s": 0.12053081147040876, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A3-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 25, "trace_sequence": 25, "timestamp": "2026-09-16T10:26:31+00:00", "logged_at": "2026-09-16T10:26:31.350000+00:00", "source_agent": null, "event_id": "obs-s6-A3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A3-t3", "wells": {"plate:A3": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15794357535082365, "rate_uM_per_s": 0.12053081147040876, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.007897178767541183, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:26:28+00:00", "acquired_at": "2026-09-16T10:26:31+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0018079621720561313}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 26, "trace_sequence": 26, "timestamp": "2026-09-16T10:26:34+00:00", "logged_at": "2026-09-16T10:26:34.350000+00:00", "source_agent": null, "event_id": "obs-s6-A3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A3-t6", "wells": {"plate:A3": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15794357535082365, "rate_uM_per_s": 0.12053081147040876, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.015794357535082366, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:26:28+00:00", "acquired_at": "2026-09-16T10:26:34+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0036159243441122625}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 27, "trace_sequence": 27, "timestamp": "2026-09-16T10:26:37+00:00", "logged_at": "2026-09-16T10:26:37.350000+00:00", "source_agent": null, "event_id": "obs-s6-A3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A3-t9", "wells": {"plate:A3": {"enzyme_volume_ul": 3.0303030303030303, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.04545454545454545, "enzyme_concentration_ng_ml": 2.0, "enzyme_concentration_M": 4.545454545454545e-11, "rate_A652_per_min": 0.15794357535082365, "rate_uM_per_s": 0.12053081147040876, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 3.0303030303030303, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.023691536302623547, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:26:28+00:00", "acquired_at": "2026-09-16T10:26:37+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005423886516168394}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 28, "trace_sequence": 28, "timestamp": "2026-09-16T10:26:49+00:00", "logged_at": "2026-09-16T10:26:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A4-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A4-hrp", "action_params": {"source": "A1", "dest": "A4", "volume_ul": 7.575757575757576, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "volume_ul": 7.575757575757576, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A4-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 29, "trace_sequence": 29, "timestamp": "2026-09-16T10:26:53+00:00", "logged_at": "2026-09-16T10:26:53.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A4-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A4-buffer", "action_params": {"source": "A3", "dest": "A4", "volume_ul": 184.42424242424244, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "volume_ul": 184.42424242424244, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A4-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 30, "trace_sequence": 30, "timestamp": "2026-09-16T10:26:59+00:00", "logged_at": "2026-09-16T10:26:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A4-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A4-tmb", "action_params": {"source": "A2", "dest": "A4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A4-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 31, "trace_sequence": 31, "timestamp": "2026-09-16T10:27:01+00:00", "logged_at": "2026-09-16T10:27:01.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A4-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A4-mix", "action_params": {"well": "A4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A4", "enzyme_volume_ul": 7.575757575757576, "tmb_volume_ul": 8.0, "buffer_volume_ul": 184.42424242424244, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.39485893837705915, "rate_uM_per_s": 0.3013270286760219, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 7.575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A4-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 32, "trace_sequence": 32, "timestamp": "2026-09-16T10:27:02+00:00", "logged_at": "2026-09-16T10:27:02.350000+00:00", "source_agent": null, "event_id": "obs-s6-A4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A4-t3", "wells": {"plate:A4": {"enzyme_volume_ul": 7.575757575757576, "tmb_volume_ul": 8.0, "buffer_volume_ul": 184.42424242424244, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.39485893837705915, "rate_uM_per_s": 0.3013270286760219, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 7.575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.01974294691885296, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:26:59+00:00", "acquired_at": "2026-09-16T10:27:02+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.004519905430140328}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 33, "trace_sequence": 33, "timestamp": "2026-09-16T10:27:05+00:00", "logged_at": "2026-09-16T10:27:05.350000+00:00", "source_agent": null, "event_id": "obs-s6-A4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A4-t6", "wells": {"plate:A4": {"enzyme_volume_ul": 7.575757575757576, "tmb_volume_ul": 8.0, "buffer_volume_ul": 184.42424242424244, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.39485893837705915, "rate_uM_per_s": 0.3013270286760219, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 7.575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.03948589383770592, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:26:59+00:00", "acquired_at": "2026-09-16T10:27:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.009039810860280656}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 34, "trace_sequence": 34, "timestamp": "2026-09-16T10:27:08+00:00", "logged_at": "2026-09-16T10:27:08.350000+00:00", "source_agent": null, "event_id": "obs-s6-A4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A4-t9", "wells": {"plate:A4": {"enzyme_volume_ul": 7.575757575757576, "tmb_volume_ul": 8.0, "buffer_volume_ul": 184.42424242424244, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.11363636363636363, "enzyme_concentration_ng_ml": 5.0, "enzyme_concentration_M": 1.1363636363636364e-10, "rate_A652_per_min": 0.39485893837705915, "rate_uM_per_s": 0.3013270286760219, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 7.575757575757576, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.059228840756558876, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:26:59+00:00", "acquired_at": "2026-09-16T10:27:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.013559716290420987}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 35, "trace_sequence": 35, "timestamp": "2026-09-16T10:27:22+00:00", "logged_at": "2026-09-16T10:27:22.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A5-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A5-hrp", "action_params": {"source": "A1", "dest": "A5", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A5-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 36, "trace_sequence": 36, "timestamp": "2026-09-16T10:27:29+00:00", "logged_at": "2026-09-16T10:27:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A5-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A5-buffer", "action_params": {"source": "A3", "dest": "A5", "volume_ul": 176.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "volume_ul": 176.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A5-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 37, "trace_sequence": 37, "timestamp": "2026-09-16T10:27:38+00:00", "logged_at": "2026-09-16T10:27:38.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A5-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A5-tmb", "action_params": {"source": "A2", "dest": "A5", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A5-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 38, "trace_sequence": 38, "timestamp": "2026-09-16T10:27:40+00:00", "logged_at": "2026-09-16T10:27:40.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A5-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A5-mix", "action_params": {"well": "A5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A5", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A5-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 39, "trace_sequence": 39, "timestamp": "2026-09-16T10:27:41+00:00", "logged_at": "2026-09-16T10:27:41.350000+00:00", "source_agent": null, "event_id": "obs-s6-A5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A5-t3", "wells": {"plate:A5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.03948589383770592, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:27:38+00:00", "acquired_at": "2026-09-16T10:27:41+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.009039810860280656}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 40, "trace_sequence": 40, "timestamp": "2026-09-16T10:27:44+00:00", "logged_at": "2026-09-16T10:27:44.350000+00:00", "source_agent": null, "event_id": "obs-s6-A5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A5-t6", "wells": {"plate:A5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.07897178767541184, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:27:38+00:00", "acquired_at": "2026-09-16T10:27:44+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.018079621720561313}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 41, "trace_sequence": 41, "timestamp": "2026-09-16T10:27:47+00:00", "logged_at": "2026-09-16T10:27:47.350000+00:00", "source_agent": null, "event_id": "obs-s6-A5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A5-t9", "wells": {"plate:A5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.11845768151311775, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:27:38+00:00", "acquired_at": "2026-09-16T10:27:47+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.027119432580841974}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 42, "trace_sequence": 42, "timestamp": "2026-09-16T10:27:57+00:00", "logged_at": "2026-09-16T10:27:57.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A6-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A6-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A6-hrp", "action_params": {"source": "A1", "dest": "A6", "volume_ul": 30.303030303030305, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "volume_ul": 30.303030303030305, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A6-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 43, "trace_sequence": 43, "timestamp": "2026-09-16T10:28:05+00:00", "logged_at": "2026-09-16T10:28:05.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A6-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A6-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A6-buffer", "action_params": {"source": "A3", "dest": "A6", "volume_ul": 161.6969696969697, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "volume_ul": 161.6969696969697, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A6-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 44, "trace_sequence": 44, "timestamp": "2026-09-16T10:28:12+00:00", "logged_at": "2026-09-16T10:28:12.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A6-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A6-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A6-tmb", "action_params": {"source": "A2", "dest": "A6", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A6-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 45, "trace_sequence": 45, "timestamp": "2026-09-16T10:28:14+00:00", "logged_at": "2026-09-16T10:28:14.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A6-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A6-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A6-mix", "action_params": {"well": "A6"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A6", "enzyme_volume_ul": 30.303030303030305, "tmb_volume_ul": 8.0, "buffer_volume_ul": 161.6969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.4214921781574128, "rate_uM_per_s": 1.0847773032336787, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 30.303030303030305, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A6-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 46, "trace_sequence": 46, "timestamp": "2026-09-16T10:28:15+00:00", "logged_at": "2026-09-16T10:28:15.350000+00:00", "source_agent": null, "event_id": "obs-s6-A6-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A6-t3", "wells": {"plate:A6": {"enzyme_volume_ul": 30.303030303030305, "tmb_volume_ul": 8.0, "buffer_volume_ul": 161.6969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.4214921781574128, "rate_uM_per_s": 1.0847773032336787, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 30.303030303030305, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.07107460890787064, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:28:12+00:00", "acquired_at": "2026-09-16T10:28:15+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01627165954850518}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 47, "trace_sequence": 47, "timestamp": "2026-09-16T10:28:18+00:00", "logged_at": "2026-09-16T10:28:18.350000+00:00", "source_agent": null, "event_id": "obs-s6-A6-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A6-t6", "wells": {"plate:A6": {"enzyme_volume_ul": 30.303030303030305, "tmb_volume_ul": 8.0, "buffer_volume_ul": 161.6969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.4214921781574128, "rate_uM_per_s": 1.0847773032336787, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 30.303030303030305, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.14214921781574127, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:28:12+00:00", "acquired_at": "2026-09-16T10:28:18+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03254331909701036}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 48, "trace_sequence": 48, "timestamp": "2026-09-16T10:28:21+00:00", "logged_at": "2026-09-16T10:28:21.350000+00:00", "source_agent": null, "event_id": "obs-s6-A6-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A6-t9", "wells": {"plate:A6": {"enzyme_volume_ul": 30.303030303030305, "tmb_volume_ul": 8.0, "buffer_volume_ul": 161.6969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.45454545454545453, "enzyme_concentration_ng_ml": 20.0, "enzyme_concentration_M": 4.5454545454545455e-10, "rate_A652_per_min": 1.4214921781574128, "rate_uM_per_s": 1.0847773032336787, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 30.303030303030305, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.21322382672361193, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:28:12+00:00", "acquired_at": "2026-09-16T10:28:21+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.048814978645515546}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 49, "trace_sequence": 49, "timestamp": "2026-09-16T10:28:32+00:00", "logged_at": "2026-09-16T10:28:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A7-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A7-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A7-hrp", "action_params": {"source": "A1", "dest": "A7", "volume_ul": 60.60606060606061, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "volume_ul": 60.60606060606061, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A7-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 50, "trace_sequence": 50, "timestamp": "2026-09-16T10:28:38+00:00", "logged_at": "2026-09-16T10:28:38.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A7-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A7-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A7-buffer", "action_params": {"source": "A3", "dest": "A7", "volume_ul": 131.39393939393938, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "volume_ul": 131.39393939393938, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A7-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 51, "trace_sequence": 51, "timestamp": "2026-09-16T10:28:47+00:00", "logged_at": "2026-09-16T10:28:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A7-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A7-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A7-tmb", "action_params": {"source": "A2", "dest": "A7", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A7-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 52, "trace_sequence": 52, "timestamp": "2026-09-16T10:28:49+00:00", "logged_at": "2026-09-16T10:28:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A7-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A7-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A7-mix", "action_params": {"well": "A7"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A7", "enzyme_volume_ul": 60.60606060606061, "tmb_volume_ul": 8.0, "buffer_volume_ul": 131.39393939393938, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.369153630262355, "rate_uM_per_s": 1.8079621720561314, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 60.60606060606061, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A7-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 53, "trace_sequence": 53, "timestamp": "2026-09-16T10:28:50+00:00", "logged_at": "2026-09-16T10:28:50.350000+00:00", "source_agent": null, "event_id": "obs-s6-A7-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A7-t3", "wells": {"plate:A7": {"enzyme_volume_ul": 60.60606060606061, "tmb_volume_ul": 8.0, "buffer_volume_ul": 131.39393939393938, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.369153630262355, "rate_uM_per_s": 1.8079621720561314, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 60.60606060606061, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.11845768151311775, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:28:47+00:00", "acquired_at": "2026-09-16T10:28:50+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.027119432580841974}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 54, "trace_sequence": 54, "timestamp": "2026-09-16T10:28:53+00:00", "logged_at": "2026-09-16T10:28:53.350000+00:00", "source_agent": null, "event_id": "obs-s6-A7-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A7-t6", "wells": {"plate:A7": {"enzyme_volume_ul": 60.60606060606061, "tmb_volume_ul": 8.0, "buffer_volume_ul": 131.39393939393938, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.369153630262355, "rate_uM_per_s": 1.8079621720561314, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 60.60606060606061, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.2369153630262355, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:28:47+00:00", "acquired_at": "2026-09-16T10:28:53+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.05423886516168395}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 55, "trace_sequence": 55, "timestamp": "2026-09-16T10:28:56+00:00", "logged_at": "2026-09-16T10:28:56.350000+00:00", "source_agent": null, "event_id": "obs-s6-A7-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A7-t9", "wells": {"plate:A7": {"enzyme_volume_ul": 60.60606060606061, "tmb_volume_ul": 8.0, "buffer_volume_ul": 131.39393939393938, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.369153630262355, "rate_uM_per_s": 1.8079621720561314, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 60.60606060606061, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.3553730445393532, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:28:47+00:00", "acquired_at": "2026-09-16T10:28:56+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.08135829774252593}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 56, "trace_sequence": 56, "timestamp": "2026-09-16T10:29:12+00:00", "logged_at": "2026-09-16T10:29:12.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A8-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A8-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A8-hrp", "action_params": {"source": "A1", "dest": "A8", "volume_ul": 121.21212121212122, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "volume_ul": 121.21212121212122, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-A8-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 57, "trace_sequence": 57, "timestamp": "2026-09-16T10:29:17+00:00", "logged_at": "2026-09-16T10:29:17.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A8-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A8-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A8-buffer", "action_params": {"source": "A3", "dest": "A8", "volume_ul": 70.78787878787878, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "volume_ul": 70.78787878787878, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-A8-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 58, "trace_sequence": 58, "timestamp": "2026-09-16T10:29:25+00:00", "logged_at": "2026-09-16T10:29:25.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A8-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-A8-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A8-tmb", "action_params": {"source": "A2", "dest": "A8", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-A8-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 59, "trace_sequence": 59, "timestamp": "2026-09-16T10:29:27+00:00", "logged_at": "2026-09-16T10:29:27.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-A8-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-A8-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-A8-mix", "action_params": {"well": "A8"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:A8", "enzyme_volume_ul": 121.21212121212122, "tmb_volume_ul": 8.0, "buffer_volume_ul": 70.78787878787878, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.5537304453935326, "rate_uM_per_s": 2.711943258084197, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 121.21212121212122, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-A8-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 60, "trace_sequence": 60, "timestamp": "2026-09-16T10:29:28+00:00", "logged_at": "2026-09-16T10:29:28.350000+00:00", "source_agent": null, "event_id": "obs-s6-A8-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A8-t3", "wells": {"plate:A8": {"enzyme_volume_ul": 121.21212121212122, "tmb_volume_ul": 8.0, "buffer_volume_ul": 70.78787878787878, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.5537304453935326, "rate_uM_per_s": 2.711943258084197, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 121.21212121212122, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.1776865222696766, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:29:25+00:00", "acquired_at": "2026-09-16T10:29:28+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.04067914887126295}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 61, "trace_sequence": 61, "timestamp": "2026-09-16T10:29:31+00:00", "logged_at": "2026-09-16T10:29:31.350000+00:00", "source_agent": null, "event_id": "obs-s6-A8-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A8-t6", "wells": {"plate:A8": {"enzyme_volume_ul": 121.21212121212122, "tmb_volume_ul": 8.0, "buffer_volume_ul": 70.78787878787878, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.5537304453935326, "rate_uM_per_s": 2.711943258084197, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 121.21212121212122, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.3553730445393532, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:29:25+00:00", "acquired_at": "2026-09-16T10:29:31+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0813582977425259}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 62, "trace_sequence": 62, "timestamp": "2026-09-16T10:29:34+00:00", "logged_at": "2026-09-16T10:29:34.350000+00:00", "source_agent": null, "event_id": "obs-s6-A8-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-A8-t9", "wells": {"plate:A8": {"enzyme_volume_ul": 121.21212121212122, "tmb_volume_ul": 8.0, "buffer_volume_ul": 70.78787878787878, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.5537304453935326, "rate_uM_per_s": 2.711943258084197, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 121.21212121212122, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true, "A652": 0.5330595668090299, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:29:25+00:00", "acquired_at": "2026-09-16T10:29:34+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.12203744661378886}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 63, "trace_sequence": 63, "timestamp": "2026-09-16T10:29:45+00:00", "logged_at": "2026-09-16T10:29:45.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B1-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B1-hrp", "action_params": {"source": "A1", "dest": "B1", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B1-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 64, "trace_sequence": 64, "timestamp": "2026-09-16T10:29:49+00:00", "logged_at": "2026-09-16T10:29:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B1-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B1-buffer", "action_params": {"source": "A3", "dest": "B1", "volume_ul": 183.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "volume_ul": 183.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B1-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 65, "trace_sequence": 65, "timestamp": "2026-09-16T10:29:58+00:00", "logged_at": "2026-09-16T10:29:58.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B1-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B1-tmb", "action_params": {"source": "A2", "dest": "B1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B1-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 66, "trace_sequence": 66, "timestamp": "2026-09-16T10:29:59+00:00", "logged_at": "2026-09-16T10:29:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B1-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B1-mix", "action_params": {"well": "B1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B1", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.25152429666424797, "rate_uM_per_s": 0.19194467083657507, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B1-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 67, "trace_sequence": 67, "timestamp": "2026-09-16T10:30:01+00:00", "logged_at": "2026-09-16T10:30:01.350000+00:00", "source_agent": null, "event_id": "obs-s6-B1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B1-t3", "wells": {"plate:B1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.25152429666424797, "rate_uM_per_s": 0.19194467083657504, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.012576214833212399, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:29:58+00:00", "acquired_at": "2026-09-16T10:30:01+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.023033360500389004}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 68, "trace_sequence": 68, "timestamp": "2026-09-16T10:30:04+00:00", "logged_at": "2026-09-16T10:30:04.350000+00:00", "source_agent": null, "event_id": "obs-s6-B1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B1-t6", "wells": {"plate:B1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.25152429666424797, "rate_uM_per_s": 0.19194467083657504, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.025152429666424798, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:29:58+00:00", "acquired_at": "2026-09-16T10:30:04+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.04606672100077801}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 69, "trace_sequence": 69, "timestamp": "2026-09-16T10:30:07+00:00", "logged_at": "2026-09-16T10:30:07.350000+00:00", "source_agent": null, "event_id": "obs-s6-B1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B1-t9", "wells": {"plate:B1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.25152429666424797, "rate_uM_per_s": 0.19194467083657504, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.0377286444996372, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:29:58+00:00", "acquired_at": "2026-09-16T10:30:07+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.06910008150116702}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 70, "trace_sequence": 70, "timestamp": "2026-09-16T10:30:21+00:00", "logged_at": "2026-09-16T10:30:21.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C1-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C1-hrp", "action_params": {"source": "A1", "dest": "C1", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C1", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C1-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 71, "trace_sequence": 71, "timestamp": "2026-09-16T10:30:29+00:00", "logged_at": "2026-09-16T10:30:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C1-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C1-buffer", "action_params": {"source": "A3", "dest": "C1", "volume_ul": 183.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C1", "volume_ul": 183.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C1-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 72, "trace_sequence": 72, "timestamp": "2026-09-16T10:30:38+00:00", "logged_at": "2026-09-16T10:30:38.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C1-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C1-tmb", "action_params": {"source": "A2", "dest": "C1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C1-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 73, "trace_sequence": 73, "timestamp": "2026-09-16T10:30:39+00:00", "logged_at": "2026-09-16T10:30:39.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C1-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C1-mix", "action_params": {"well": "C1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C1", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.22680075876812458, "rate_uM_per_s": 0.17307750211242717, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C1-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 74, "trace_sequence": 74, "timestamp": "2026-09-16T10:30:41+00:00", "logged_at": "2026-09-16T10:30:41.350000+00:00", "source_agent": null, "event_id": "obs-s6-C1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C1-t3", "wells": {"plate:C1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.22680075876812458, "rate_uM_per_s": 0.17307750211242717, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.01134003793840623, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:30:38+00:00", "acquired_at": "2026-09-16T10:30:41+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.020769300253491264}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 75, "trace_sequence": 75, "timestamp": "2026-09-16T10:30:44+00:00", "logged_at": "2026-09-16T10:30:44.350000+00:00", "source_agent": null, "event_id": "obs-s6-C1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C1-t6", "wells": {"plate:C1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.22680075876812458, "rate_uM_per_s": 0.17307750211242717, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.02268007587681246, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:30:38+00:00", "acquired_at": "2026-09-16T10:30:44+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.04153860050698253}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 76, "trace_sequence": 76, "timestamp": "2026-09-16T10:30:47+00:00", "logged_at": "2026-09-16T10:30:47.350000+00:00", "source_agent": null, "event_id": "obs-s6-C1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C1-t9", "wells": {"plate:C1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.22680075876812458, "rate_uM_per_s": 0.17307750211242717, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.03402011381521868, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:30:38+00:00", "acquired_at": "2026-09-16T10:30:47+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.06230790076047378}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 77, "trace_sequence": 77, "timestamp": "2026-09-16T10:30:59+00:00", "logged_at": "2026-09-16T10:30:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D1-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D1-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D1-hrp", "action_params": {"source": "A1", "dest": "D1", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D1", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D1-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 78, "trace_sequence": 78, "timestamp": "2026-09-16T10:31:06+00:00", "logged_at": "2026-09-16T10:31:06.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D1-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D1-buffer", "action_params": {"source": "A3", "dest": "D1", "volume_ul": 183.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D1", "volume_ul": 183.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D1-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 79, "trace_sequence": 79, "timestamp": "2026-09-16T10:31:15+00:00", "logged_at": "2026-09-16T10:31:15.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D1-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D1-tmb", "action_params": {"source": "A2", "dest": "D1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D1-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 80, "trace_sequence": 80, "timestamp": "2026-09-16T10:31:17+00:00", "logged_at": "2026-09-16T10:31:17.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D1-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D1-mix", "action_params": {"well": "D1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D1", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.23916252771618632, "rate_uM_per_s": 0.18251108647450112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-D1-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 81, "trace_sequence": 81, "timestamp": "2026-09-16T10:31:18+00:00", "logged_at": "2026-09-16T10:31:18.350000+00:00", "source_agent": null, "event_id": "obs-s6-D1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D1-t3", "wells": {"plate:D1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.23916252771618632, "rate_uM_per_s": 0.18251108647450112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.011958126385809316, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:31:15+00:00", "acquired_at": "2026-09-16T10:31:18+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.021901330376940135}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 82, "trace_sequence": 82, "timestamp": "2026-09-16T10:31:21+00:00", "logged_at": "2026-09-16T10:31:21.350000+00:00", "source_agent": null, "event_id": "obs-s6-D1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D1-t6", "wells": {"plate:D1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.23916252771618632, "rate_uM_per_s": 0.18251108647450112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.023916252771618633, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:31:15+00:00", "acquired_at": "2026-09-16T10:31:21+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.04380266075388027}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 83, "trace_sequence": 83, "timestamp": "2026-09-16T10:31:24+00:00", "logged_at": "2026-09-16T10:31:24.350000+00:00", "source_agent": null, "event_id": "obs-s6-D1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D1-t9", "wells": {"plate:D1": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 1.0, "buffer_volume_ul": 183.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.23916252771618632, "rate_uM_per_s": 0.18251108647450112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.18251108647450112, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.03587437915742795, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:31:15+00:00", "acquired_at": "2026-09-16T10:31:24+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.06570399113082041}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 84, "trace_sequence": 84, "timestamp": "2026-09-16T10:31:32+00:00", "logged_at": "2026-09-16T10:31:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B2-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B2-hrp", "action_params": {"source": "A1", "dest": "B2", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B2-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 85, "trace_sequence": 85, "timestamp": "2026-09-16T10:31:39+00:00", "logged_at": "2026-09-16T10:31:39.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B2-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B2-buffer", "action_params": {"source": "A3", "dest": "B2", "volume_ul": 182.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "volume_ul": 182.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B2-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 86, "trace_sequence": 86, "timestamp": "2026-09-16T10:31:47+00:00", "logged_at": "2026-09-16T10:31:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B2-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B2-tmb", "action_params": {"source": "A2", "dest": "B2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B2-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 87, "trace_sequence": 87, "timestamp": "2026-09-16T10:31:48+00:00", "logged_at": "2026-09-16T10:31:48.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B2-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B2-mix", "action_params": {"well": "B2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B2", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.409888673124966, "rate_uM_per_s": 0.3127966064750961, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B2-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 88, "trace_sequence": 88, "timestamp": "2026-09-16T10:31:50+00:00", "logged_at": "2026-09-16T10:31:50.350000+00:00", "source_agent": null, "event_id": "obs-s6-B2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B2-t3", "wells": {"plate:B2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.409888673124966, "rate_uM_per_s": 0.31279660647509616, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.0204944336562483, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:31:47+00:00", "acquired_at": "2026-09-16T10:31:50+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01876779638850577}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 89, "trace_sequence": 89, "timestamp": "2026-09-16T10:31:53+00:00", "logged_at": "2026-09-16T10:31:53.350000+00:00", "source_agent": null, "event_id": "obs-s6-B2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B2-t6", "wells": {"plate:B2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.409888673124966, "rate_uM_per_s": 0.31279660647509616, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.0409888673124966, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:31:47+00:00", "acquired_at": "2026-09-16T10:31:53+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03753559277701154}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 90, "trace_sequence": 90, "timestamp": "2026-09-16T10:31:56+00:00", "logged_at": "2026-09-16T10:31:56.350000+00:00", "source_agent": null, "event_id": "obs-s6-B2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B2-t9", "wells": {"plate:B2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.409888673124966, "rate_uM_per_s": 0.31279660647509616, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.0614833009687449, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:31:47+00:00", "acquired_at": "2026-09-16T10:31:56+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.056303389165517304}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 91, "trace_sequence": 91, "timestamp": "2026-09-16T10:32:07+00:00", "logged_at": "2026-09-16T10:32:07.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C2-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C2-hrp", "action_params": {"source": "A1", "dest": "C2", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C2", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C2-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 92, "trace_sequence": 92, "timestamp": "2026-09-16T10:32:16+00:00", "logged_at": "2026-09-16T10:32:16.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C2-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C2-buffer", "action_params": {"source": "A3", "dest": "C2", "volume_ul": 182.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C2", "volume_ul": 182.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C2-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 93, "trace_sequence": 93, "timestamp": "2026-09-16T10:32:22+00:00", "logged_at": "2026-09-16T10:32:22.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C2-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C2-tmb", "action_params": {"source": "A2", "dest": "C2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C2-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 94, "trace_sequence": 94, "timestamp": "2026-09-16T10:32:23+00:00", "logged_at": "2026-09-16T10:32:23.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C2-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C2-mix", "action_params": {"well": "C2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C2", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.38516513522884255, "rate_uM_per_s": 0.29392943775094815, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C2-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 95, "trace_sequence": 95, "timestamp": "2026-09-16T10:32:25+00:00", "logged_at": "2026-09-16T10:32:25.350000+00:00", "source_agent": null, "event_id": "obs-s6-C2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C2-t3", "wells": {"plate:C2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.38516513522884255, "rate_uM_per_s": 0.2939294377509482, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.019258256761442126, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:32:22+00:00", "acquired_at": "2026-09-16T10:32:25+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01763576626505689}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 96, "trace_sequence": 96, "timestamp": "2026-09-16T10:32:28+00:00", "logged_at": "2026-09-16T10:32:28.350000+00:00", "source_agent": null, "event_id": "obs-s6-C2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C2-t6", "wells": {"plate:C2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.38516513522884255, "rate_uM_per_s": 0.2939294377509482, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.03851651352288425, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:32:22+00:00", "acquired_at": "2026-09-16T10:32:28+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03527153253011378}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 97, "trace_sequence": 97, "timestamp": "2026-09-16T10:32:31+00:00", "logged_at": "2026-09-16T10:32:31.350000+00:00", "source_agent": null, "event_id": "obs-s6-C2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C2-t9", "wells": {"plate:C2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.38516513522884255, "rate_uM_per_s": 0.2939294377509482, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.057774770284326385, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:32:22+00:00", "acquired_at": "2026-09-16T10:32:31+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.05290729879517068}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 98, "trace_sequence": 98, "timestamp": "2026-09-16T10:32:41+00:00", "logged_at": "2026-09-16T10:32:41.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D2-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D2-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D2-hrp", "action_params": {"source": "A1", "dest": "D2", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D2", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D2-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 99, "trace_sequence": 99, "timestamp": "2026-09-16T10:32:49+00:00", "logged_at": "2026-09-16T10:32:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D2-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D2-buffer", "action_params": {"source": "A3", "dest": "D2", "volume_ul": 182.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D2", "volume_ul": 182.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D2-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 100, "trace_sequence": 100, "timestamp": "2026-09-16T10:32:57+00:00", "logged_at": "2026-09-16T10:32:57.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D2-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D2-tmb", "action_params": {"source": "A2", "dest": "D2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D2-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 101, "trace_sequence": 101, "timestamp": "2026-09-16T10:32:59+00:00", "logged_at": "2026-09-16T10:32:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D2-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D2-mix", "action_params": {"well": "D2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D2", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.3975269041769042, "rate_uM_per_s": 0.30336302211302213, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-D2-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 102, "trace_sequence": 102, "timestamp": "2026-09-16T10:33:00+00:00", "logged_at": "2026-09-16T10:33:00.350000+00:00", "source_agent": null, "event_id": "obs-s6-D2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D2-t3", "wells": {"plate:D2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.3975269041769042, "rate_uM_per_s": 0.3033630221130221, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.019876345208845212, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:32:57+00:00", "acquired_at": "2026-09-16T10:33:00+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.018201781326781324}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 103, "trace_sequence": 103, "timestamp": "2026-09-16T10:33:03+00:00", "logged_at": "2026-09-16T10:33:03.350000+00:00", "source_agent": null, "event_id": "obs-s6-D2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D2-t6", "wells": {"plate:D2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.3975269041769042, "rate_uM_per_s": 0.3033630221130221, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.039752690417690424, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:32:57+00:00", "acquired_at": "2026-09-16T10:33:03+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03640356265356265}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 104, "trace_sequence": 104, "timestamp": "2026-09-16T10:33:06+00:00", "logged_at": "2026-09-16T10:33:06.350000+00:00", "source_agent": null, "event_id": "obs-s6-D2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D2-t9", "wells": {"plate:D2": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 2.0, "buffer_volume_ul": 182.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.3975269041769042, "rate_uM_per_s": 0.3033630221130221, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.30336302211302213, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05962903562653563, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:32:57+00:00", "acquired_at": "2026-09-16T10:33:06+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.05460534398034397}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 105, "trace_sequence": 105, "timestamp": "2026-09-16T10:33:22+00:00", "logged_at": "2026-09-16T10:33:22.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B3-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B3-hrp", "action_params": {"source": "A1", "dest": "B3", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B3-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 106, "trace_sequence": 106, "timestamp": "2026-09-16T10:33:31+00:00", "logged_at": "2026-09-16T10:33:31.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B3-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B3-buffer", "action_params": {"source": "A3", "dest": "B3", "volume_ul": 180.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "volume_ul": 180.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B3-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 107, "trace_sequence": 107, "timestamp": "2026-09-16T10:33:36+00:00", "logged_at": "2026-09-16T10:33:36.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B3-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B3-tmb", "action_params": {"source": "A2", "dest": "B3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B3-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 108, "trace_sequence": 108, "timestamp": "2026-09-16T10:33:38+00:00", "logged_at": "2026-09-16T10:33:38.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B3-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B3-mix", "action_params": {"well": "B3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B3", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.6066444135761611, "rate_uM_per_s": 0.46294598105628887, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B3-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 109, "trace_sequence": 109, "timestamp": "2026-09-16T10:33:39+00:00", "logged_at": "2026-09-16T10:33:39.350000+00:00", "source_agent": null, "event_id": "obs-s6-B3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B3-t3", "wells": {"plate:B3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.6066444135761611, "rate_uM_per_s": 0.46294598105628887, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.03033222067880805, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:33:36+00:00", "acquired_at": "2026-09-16T10:33:39+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.013888379431688667}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 110, "trace_sequence": 110, "timestamp": "2026-09-16T10:33:42+00:00", "logged_at": "2026-09-16T10:33:42.350000+00:00", "source_agent": null, "event_id": "obs-s6-B3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B3-t6", "wells": {"plate:B3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.6066444135761611, "rate_uM_per_s": 0.46294598105628887, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.0606644413576161, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:33:36+00:00", "acquired_at": "2026-09-16T10:33:42+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.027776758863377334}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 111, "trace_sequence": 111, "timestamp": "2026-09-16T10:33:45+00:00", "logged_at": "2026-09-16T10:33:45.350000+00:00", "source_agent": null, "event_id": "obs-s6-B3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B3-t9", "wells": {"plate:B3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.6066444135761611, "rate_uM_per_s": 0.46294598105628887, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.09099666203642416, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:33:36+00:00", "acquired_at": "2026-09-16T10:33:45+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.041665138295066}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 112, "trace_sequence": 112, "timestamp": "2026-09-16T10:33:57+00:00", "logged_at": "2026-09-16T10:33:57.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C3-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C3-hrp", "action_params": {"source": "A1", "dest": "C3", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C3", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C3-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 113, "trace_sequence": 113, "timestamp": "2026-09-16T10:34:02+00:00", "logged_at": "2026-09-16T10:34:02.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C3-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C3-buffer", "action_params": {"source": "A3", "dest": "C3", "volume_ul": 180.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C3", "volume_ul": 180.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C3-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 114, "trace_sequence": 114, "timestamp": "2026-09-16T10:34:11+00:00", "logged_at": "2026-09-16T10:34:11.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C3-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C3-tmb", "action_params": {"source": "A2", "dest": "C3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C3-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 115, "trace_sequence": 115, "timestamp": "2026-09-16T10:34:12+00:00", "logged_at": "2026-09-16T10:34:12.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C3-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C3-mix", "action_params": {"well": "C3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C3", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.5819208756800376, "rate_uM_per_s": 0.4440788123321409, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C3-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 116, "trace_sequence": 116, "timestamp": "2026-09-16T10:34:14+00:00", "logged_at": "2026-09-16T10:34:14.350000+00:00", "source_agent": null, "event_id": "obs-s6-C3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C3-t3", "wells": {"plate:C3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.5819208756800376, "rate_uM_per_s": 0.44407881233214097, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.02909604378400188, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:34:11+00:00", "acquired_at": "2026-09-16T10:34:14+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01332236436996423}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 117, "trace_sequence": 117, "timestamp": "2026-09-16T10:34:17+00:00", "logged_at": "2026-09-16T10:34:17.350000+00:00", "source_agent": null, "event_id": "obs-s6-C3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C3-t6", "wells": {"plate:C3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.5819208756800376, "rate_uM_per_s": 0.44407881233214097, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05819208756800376, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:34:11+00:00", "acquired_at": "2026-09-16T10:34:17+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.02664472873992846}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 118, "trace_sequence": 118, "timestamp": "2026-09-16T10:34:20+00:00", "logged_at": "2026-09-16T10:34:20.350000+00:00", "source_agent": null, "event_id": "obs-s6-C3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C3-t9", "wells": {"plate:C3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.5819208756800376, "rate_uM_per_s": 0.44407881233214097, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.08728813135200562, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:34:11+00:00", "acquired_at": "2026-09-16T10:34:20+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03996709310989269}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 119, "trace_sequence": 119, "timestamp": "2026-09-16T10:34:31+00:00", "logged_at": "2026-09-16T10:34:31.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D3-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D3-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D3-hrp", "action_params": {"source": "A1", "dest": "D3", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D3", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D3-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 120, "trace_sequence": 120, "timestamp": "2026-09-16T10:34:38+00:00", "logged_at": "2026-09-16T10:34:38.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D3-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D3-buffer", "action_params": {"source": "A3", "dest": "D3", "volume_ul": 180.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D3", "volume_ul": 180.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D3-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 121, "trace_sequence": 121, "timestamp": "2026-09-16T10:34:47+00:00", "logged_at": "2026-09-16T10:34:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D3-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D3-tmb", "action_params": {"source": "A2", "dest": "D3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D3-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 122, "trace_sequence": 122, "timestamp": "2026-09-16T10:34:49+00:00", "logged_at": "2026-09-16T10:34:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D3-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D3-mix", "action_params": {"well": "D3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D3", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.5942826446280993, "rate_uM_per_s": 0.4535123966942149, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.4535123966942149, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": false, "initial_rate_eligible": false}}, "result_observation_ids": ["obs-plan-s6-D3-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 123, "trace_sequence": 123, "timestamp": "2026-09-16T10:34:49+00:00", "logged_at": "2026-09-16T10:34:49.350000+00:00", "source_agent": "human", "event_id": "s6-spike", "event_type": "external_perturbation", "message": "The spiked well is chosen among the substrate series only. The HRP titration wells are not in that count. D3 is preparation 9 of the 21-well substrate series, inside the first 15. After enzyme and substrate were combined, a person added an extra 5 uL of TMB to D3.", "rationale": "The spiked well is chosen among the substrate series only. The HRP titration wells are not in that count. D3 is preparation 9 of the 21-well substrate series, inside the first 15. After enzyme and substrate were combined, a person added an extra 5 uL of TMB to D3.", "payload": {"well": "D3", "added_volume_ul": 5, "added_substance": "TMB", "announced": false, "disclosed_to_agents": false, "extra_tmb_ul": 5, "total_volume_ul": 205.0, "substrate_uM": 219.5121951219512, "enzyme_concentration_ng_ml": 9.75609756097561, "selection_population": "substrate_series", "selection_limit": 15, "substrate_series_index": 9, "substrate_series_size": 21, "titration_included": false}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 124, "trace_sequence": 124, "timestamp": "2026-09-16T10:34:50+00:00", "logged_at": "2026-09-16T10:34:50.350000+00:00", "source_agent": null, "event_id": "obs-s6-D3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D3-t3", "wells": {"plate:D3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7936565676247692, "rate_uM_per_s": 0.6056597738284257, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "mm", "used_for_fit": false, "initial_rate_eligible": false, "A652": 0.03968282838123846, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:34:47+00:00", "acquired_at": "2026-09-16T10:34:50+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01816979321485277, "suspect_outlier": true}}, "note": "D3 is much faster than the other wells prepared at the same nominal substrate. It looks as if that one well was contaminated. I do not know what reached it."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 125, "trace_sequence": 125, "timestamp": "2026-09-16T10:34:53+00:00", "logged_at": "2026-09-16T10:34:53.350000+00:00", "source_agent": null, "event_id": "obs-s6-D3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D3-t6", "wells": {"plate:D3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7936565676247692, "rate_uM_per_s": 0.6056597738284257, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "mm", "used_for_fit": false, "initial_rate_eligible": false, "A652": 0.07936565676247692, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:34:47+00:00", "acquired_at": "2026-09-16T10:34:53+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.03633958642970554, "suspect_outlier": true}}, "note": "D3 is much faster than the other wells prepared at the same nominal substrate. It looks as if that one well was contaminated. I do not know what reached it."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 126, "trace_sequence": 126, "timestamp": "2026-09-16T10:34:56+00:00", "logged_at": "2026-09-16T10:34:56.350000+00:00", "source_agent": null, "event_id": "obs-s6-D3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D3-t9", "wells": {"plate:D3": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 4.0, "buffer_volume_ul": 180.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7936565676247692, "rate_uM_per_s": 0.6056597738284257, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "series": "mm", "used_for_fit": false, "initial_rate_eligible": false, "A652": 0.11904848514371538, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:34:47+00:00", "acquired_at": "2026-09-16T10:34:56+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.05450937964455831, "suspect_outlier": true}}, "note": "D3 is much faster than the other wells prepared at the same nominal substrate. It looks as if that one well was contaminated. I do not know what reached it."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 127, "trace_sequence": 127, "timestamp": "2026-09-16T10:35:11+00:00", "logged_at": "2026-09-16T10:35:11.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B4-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B4-hrp", "action_params": {"source": "A1", "dest": "B4", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B4-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 128, "trace_sequence": 128, "timestamp": "2026-09-16T10:35:16+00:00", "logged_at": "2026-09-16T10:35:16.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B4-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B4-buffer", "action_params": {"source": "A3", "dest": "B4", "volume_ul": 176.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "volume_ul": 176.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B4-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 129, "trace_sequence": 129, "timestamp": "2026-09-16T10:35:24+00:00", "logged_at": "2026-09-16T10:35:24.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B4-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B4-tmb", "action_params": {"source": "A2", "dest": "B4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B4-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 130, "trace_sequence": 130, "timestamp": "2026-09-16T10:35:26+00:00", "logged_at": "2026-09-16T10:35:26.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B4-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B4-mix", "action_params": {"well": "B4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B4", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.8020796457021803, "rate_uM_per_s": 0.6120876417141179, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B4-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 131, "trace_sequence": 131, "timestamp": "2026-09-16T10:35:27+00:00", "logged_at": "2026-09-16T10:35:27.350000+00:00", "source_agent": null, "event_id": "obs-s6-B4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B4-t3", "wells": {"plate:B4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.8020796457021803, "rate_uM_per_s": 0.6120876417141179, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.040103982285109016, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:35:24+00:00", "acquired_at": "2026-09-16T10:35:27+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.009181314625711768}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 132, "trace_sequence": 132, "timestamp": "2026-09-16T10:35:30+00:00", "logged_at": "2026-09-16T10:35:30.350000+00:00", "source_agent": null, "event_id": "obs-s6-B4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B4-t6", "wells": {"plate:B4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.8020796457021803, "rate_uM_per_s": 0.6120876417141179, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.08020796457021803, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:35:24+00:00", "acquired_at": "2026-09-16T10:35:30+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.018362629251423537}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 133, "trace_sequence": 133, "timestamp": "2026-09-16T10:35:33+00:00", "logged_at": "2026-09-16T10:35:33.350000+00:00", "source_agent": null, "event_id": "obs-s6-B4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B4-t9", "wells": {"plate:B4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.8020796457021803, "rate_uM_per_s": 0.6120876417141179, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.12031194685532705, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:35:24+00:00", "acquired_at": "2026-09-16T10:35:33+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.027543943877135307}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 134, "trace_sequence": 134, "timestamp": "2026-09-16T10:35:49+00:00", "logged_at": "2026-09-16T10:35:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C4-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C4-hrp", "action_params": {"source": "A1", "dest": "C4", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C4", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C4-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 135, "trace_sequence": 135, "timestamp": "2026-09-16T10:35:55+00:00", "logged_at": "2026-09-16T10:35:55.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C4-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C4-buffer", "action_params": {"source": "A3", "dest": "C4", "volume_ul": 176.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C4", "volume_ul": 176.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C4-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 136, "trace_sequence": 136, "timestamp": "2026-09-16T10:35:59+00:00", "logged_at": "2026-09-16T10:35:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C4-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C4-tmb", "action_params": {"source": "A2", "dest": "C4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C4-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 137, "trace_sequence": 137, "timestamp": "2026-09-16T10:36:00+00:00", "logged_at": "2026-09-16T10:36:00.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C4-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C4-mix", "action_params": {"well": "C4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C4", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7773561078060567, "rate_uM_per_s": 0.5932204729899699, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C4-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 138, "trace_sequence": 138, "timestamp": "2026-09-16T10:36:02+00:00", "logged_at": "2026-09-16T10:36:02.350000+00:00", "source_agent": null, "event_id": "obs-s6-C4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C4-t3", "wells": {"plate:C4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7773561078060567, "rate_uM_per_s": 0.5932204729899699, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.03886780539030284, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:35:59+00:00", "acquired_at": "2026-09-16T10:36:02+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00889830709484955}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 139, "trace_sequence": 139, "timestamp": "2026-09-16T10:36:05+00:00", "logged_at": "2026-09-16T10:36:05.350000+00:00", "source_agent": null, "event_id": "obs-s6-C4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C4-t6", "wells": {"plate:C4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7773561078060567, "rate_uM_per_s": 0.5932204729899699, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.07773561078060567, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:35:59+00:00", "acquired_at": "2026-09-16T10:36:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0177966141896991}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 140, "trace_sequence": 140, "timestamp": "2026-09-16T10:36:08+00:00", "logged_at": "2026-09-16T10:36:08.350000+00:00", "source_agent": null, "event_id": "obs-s6-C4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C4-t9", "wells": {"plate:C4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7773561078060567, "rate_uM_per_s": 0.5932204729899699, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.1166034161709085, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:35:59+00:00", "acquired_at": "2026-09-16T10:36:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.026694921284548645}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 141, "trace_sequence": 141, "timestamp": "2026-09-16T10:36:17+00:00", "logged_at": "2026-09-16T10:36:17.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D4-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D4-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D4-hrp", "action_params": {"source": "A1", "dest": "D4", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D4", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D4-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 142, "trace_sequence": 142, "timestamp": "2026-09-16T10:36:22+00:00", "logged_at": "2026-09-16T10:36:22.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D4-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D4-buffer", "action_params": {"source": "A3", "dest": "D4", "volume_ul": 176.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D4", "volume_ul": 176.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D4-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 143, "trace_sequence": 143, "timestamp": "2026-09-16T10:36:29+00:00", "logged_at": "2026-09-16T10:36:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D4-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D4-tmb", "action_params": {"source": "A2", "dest": "D4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D4-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 144, "trace_sequence": 144, "timestamp": "2026-09-16T10:36:30+00:00", "logged_at": "2026-09-16T10:36:30.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D4-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D4-mix", "action_params": {"well": "D4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D4", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520439, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-D4-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 145, "trace_sequence": 145, "timestamp": "2026-09-16T10:36:32+00:00", "logged_at": "2026-09-16T10:36:32.350000+00:00", "source_agent": null, "event_id": "obs-s6-D4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D4-t3", "wells": {"plate:D4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.03948589383770592, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:36:29+00:00", "acquired_at": "2026-09-16T10:36:32+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.009039810860280656}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 146, "trace_sequence": 146, "timestamp": "2026-09-16T10:36:35+00:00", "logged_at": "2026-09-16T10:36:35.350000+00:00", "source_agent": null, "event_id": "obs-s6-D4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D4-t6", "wells": {"plate:D4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.07897178767541184, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:36:29+00:00", "acquired_at": "2026-09-16T10:36:35+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.018079621720561313}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 147, "trace_sequence": 147, "timestamp": "2026-09-16T10:36:38+00:00", "logged_at": "2026-09-16T10:36:38.350000+00:00", "source_agent": null, "event_id": "obs-s6-D4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D4-t9", "wells": {"plate:D4": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.6026540573520439, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.11845768151311775, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:36:29+00:00", "acquired_at": "2026-09-16T10:36:38+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.027119432580841974}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 148, "trace_sequence": 148, "timestamp": "2026-09-16T10:36:47+00:00", "logged_at": "2026-09-16T10:36:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B5-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B5-hrp", "action_params": {"source": "A1", "dest": "B5", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B5-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 149, "trace_sequence": 149, "timestamp": "2026-09-16T10:36:54+00:00", "logged_at": "2026-09-16T10:36:54.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B5-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B5-buffer", "action_params": {"source": "A3", "dest": "B5", "volume_ul": 168.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "volume_ul": 168.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B5-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 150, "trace_sequence": 150, "timestamp": "2026-09-16T10:36:58+00:00", "logged_at": "2026-09-16T10:36:58.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B5-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B5-tmb", "action_params": {"source": "A2", "dest": "B5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B5-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 151, "trace_sequence": 151, "timestamp": "2026-09-16T10:36:59+00:00", "logged_at": "2026-09-16T10:36:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B5-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B5-mix", "action_params": {"well": "B5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B5", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9574859748626293, "rate_uM_per_s": 0.7306822152492591, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B5-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 152, "trace_sequence": 152, "timestamp": "2026-09-16T10:37:01+00:00", "logged_at": "2026-09-16T10:37:01.350000+00:00", "source_agent": null, "event_id": "obs-s6-B5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B5-t3", "wells": {"plate:B5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9574859748626293, "rate_uM_per_s": 0.730682215249259, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.04787429874313146, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:36:58+00:00", "acquired_at": "2026-09-16T10:37:01+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005480116614369443}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 153, "trace_sequence": 153, "timestamp": "2026-09-16T10:37:04+00:00", "logged_at": "2026-09-16T10:37:04.350000+00:00", "source_agent": null, "event_id": "obs-s6-B5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B5-t6", "wells": {"plate:B5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9574859748626293, "rate_uM_per_s": 0.730682215249259, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.09574859748626292, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:36:58+00:00", "acquired_at": "2026-09-16T10:37:04+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.010960233228738885}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 154, "trace_sequence": 154, "timestamp": "2026-09-16T10:37:07+00:00", "logged_at": "2026-09-16T10:37:07.350000+00:00", "source_agent": null, "event_id": "obs-s6-B5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B5-t9", "wells": {"plate:B5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9574859748626293, "rate_uM_per_s": 0.730682215249259, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.1436228962293944, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:36:58+00:00", "acquired_at": "2026-09-16T10:37:07+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.01644034984310833}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 155, "trace_sequence": 155, "timestamp": "2026-09-16T10:37:16+00:00", "logged_at": "2026-09-16T10:37:16.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C5-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C5-hrp", "action_params": {"source": "A1", "dest": "C5", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C5", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C5-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 156, "trace_sequence": 156, "timestamp": "2026-09-16T10:37:21+00:00", "logged_at": "2026-09-16T10:37:21.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C5-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C5-buffer", "action_params": {"source": "A3", "dest": "C5", "volume_ul": 168.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C5", "volume_ul": 168.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C5-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 157, "trace_sequence": 157, "timestamp": "2026-09-16T10:37:30+00:00", "logged_at": "2026-09-16T10:37:30.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C5-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C5-tmb", "action_params": {"source": "A2", "dest": "C5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C5-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 158, "trace_sequence": 158, "timestamp": "2026-09-16T10:37:32+00:00", "logged_at": "2026-09-16T10:37:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C5-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C5-mix", "action_params": {"well": "C5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C5", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9327624369665058, "rate_uM_per_s": 0.7118150465251112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C5-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 159, "trace_sequence": 159, "timestamp": "2026-09-16T10:37:33+00:00", "logged_at": "2026-09-16T10:37:33.350000+00:00", "source_agent": null, "event_id": "obs-s6-C5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C5-t3", "wells": {"plate:C5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9327624369665058, "rate_uM_per_s": 0.7118150465251112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.04663812184832529, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:37:30+00:00", "acquired_at": "2026-09-16T10:37:33+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005338612848938333}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 160, "trace_sequence": 160, "timestamp": "2026-09-16T10:37:36+00:00", "logged_at": "2026-09-16T10:37:36.350000+00:00", "source_agent": null, "event_id": "obs-s6-C5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C5-t6", "wells": {"plate:C5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9327624369665058, "rate_uM_per_s": 0.7118150465251112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.09327624369665058, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:37:30+00:00", "acquired_at": "2026-09-16T10:37:36+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.010677225697876666}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 161, "trace_sequence": 161, "timestamp": "2026-09-16T10:37:39+00:00", "logged_at": "2026-09-16T10:37:39.350000+00:00", "source_agent": null, "event_id": "obs-s6-C5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C5-t9", "wells": {"plate:C5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9327624369665058, "rate_uM_per_s": 0.7118150465251112, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.13991436554497585, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:37:30+00:00", "acquired_at": "2026-09-16T10:37:39+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.016015838546815}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 162, "trace_sequence": 162, "timestamp": "2026-09-16T10:37:47+00:00", "logged_at": "2026-09-16T10:37:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D5-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D5-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D5-hrp", "action_params": {"source": "A1", "dest": "D5", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D5", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D5-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 163, "trace_sequence": 163, "timestamp": "2026-09-16T10:37:54+00:00", "logged_at": "2026-09-16T10:37:54.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D5-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D5-buffer", "action_params": {"source": "A3", "dest": "D5", "volume_ul": 168.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D5", "volume_ul": 168.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D5-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 164, "trace_sequence": 164, "timestamp": "2026-09-16T10:38:02+00:00", "logged_at": "2026-09-16T10:38:02.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D5-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D5-tmb", "action_params": {"source": "A2", "dest": "D5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D5-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 165, "trace_sequence": 165, "timestamp": "2026-09-16T10:38:03+00:00", "logged_at": "2026-09-16T10:38:03.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D5-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D5-mix", "action_params": {"well": "D5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D5", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9451242059145675, "rate_uM_per_s": 0.7212486308871852, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-D5-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 166, "trace_sequence": 166, "timestamp": "2026-09-16T10:38:05+00:00", "logged_at": "2026-09-16T10:38:05.350000+00:00", "source_agent": null, "event_id": "obs-s6-D5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D5-t3", "wells": {"plate:D5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9451242059145675, "rate_uM_per_s": 0.721248630887185, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.04725621029572837, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:38:02+00:00", "acquired_at": "2026-09-16T10:38:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0054093647316538875}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 167, "trace_sequence": 167, "timestamp": "2026-09-16T10:38:08+00:00", "logged_at": "2026-09-16T10:38:08.350000+00:00", "source_agent": null, "event_id": "obs-s6-D5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D5-t6", "wells": {"plate:D5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9451242059145675, "rate_uM_per_s": 0.721248630887185, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.09451242059145674, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:38:02+00:00", "acquired_at": "2026-09-16T10:38:08+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.010818729463307775}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 168, "trace_sequence": 168, "timestamp": "2026-09-16T10:38:11+00:00", "logged_at": "2026-09-16T10:38:11.350000+00:00", "source_agent": null, "event_id": "obs-s6-D5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D5-t9", "wells": {"plate:D5": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 16.0, "buffer_volume_ul": 168.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 0.9451242059145675, "rate_uM_per_s": 0.721248630887185, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.7212486308871852, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.1417686308871851, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:38:02+00:00", "acquired_at": "2026-09-16T10:38:11+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.016228094194961663}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 169, "trace_sequence": 169, "timestamp": "2026-09-16T10:38:25+00:00", "logged_at": "2026-09-16T10:38:25.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B6-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B6-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B6-hrp", "action_params": {"source": "A1", "dest": "B6", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B6", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B6-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 170, "trace_sequence": 170, "timestamp": "2026-09-16T10:38:29+00:00", "logged_at": "2026-09-16T10:38:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B6-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B6-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B6-buffer", "action_params": {"source": "A3", "dest": "B6", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B6", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B6-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 171, "trace_sequence": 171, "timestamp": "2026-09-16T10:38:36+00:00", "logged_at": "2026-09-16T10:38:36.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B6-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B6-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B6-tmb", "action_params": {"source": "A2", "dest": "B6", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B6", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B6-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 172, "trace_sequence": 172, "timestamp": "2026-09-16T10:38:37+00:00", "logged_at": "2026-09-16T10:38:37.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B6-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B6-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B6-mix", "action_params": {"well": "B6"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B6", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0606287055749095, "rate_uM_per_s": 0.809393090334943, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B6-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 173, "trace_sequence": 173, "timestamp": "2026-09-16T10:38:39+00:00", "logged_at": "2026-09-16T10:38:39.350000+00:00", "source_agent": null, "event_id": "obs-s6-B6-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B6-t3", "wells": {"plate:B6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0606287055749095, "rate_uM_per_s": 0.809393090334943, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05303143527874547, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:38:36+00:00", "acquired_at": "2026-09-16T10:38:39+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.003035224088756036}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 174, "trace_sequence": 174, "timestamp": "2026-09-16T10:38:42+00:00", "logged_at": "2026-09-16T10:38:42.350000+00:00", "source_agent": null, "event_id": "obs-s6-B6-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B6-t6", "wells": {"plate:B6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0606287055749095, "rate_uM_per_s": 0.809393090334943, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.10606287055749095, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:38:36+00:00", "acquired_at": "2026-09-16T10:38:42+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.006070448177512072}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 175, "trace_sequence": 175, "timestamp": "2026-09-16T10:38:45+00:00", "logged_at": "2026-09-16T10:38:45.350000+00:00", "source_agent": null, "event_id": "obs-s6-B6-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B6-t9", "wells": {"plate:B6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0606287055749095, "rate_uM_per_s": 0.809393090334943, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.15909430583623643, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:38:36+00:00", "acquired_at": "2026-09-16T10:38:45+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.009105672266268109}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 176, "trace_sequence": 176, "timestamp": "2026-09-16T10:38:59+00:00", "logged_at": "2026-09-16T10:38:59.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C6-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C6-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C6-hrp", "action_params": {"source": "A1", "dest": "C6", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C6", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C6-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 177, "trace_sequence": 177, "timestamp": "2026-09-16T10:39:07+00:00", "logged_at": "2026-09-16T10:39:07.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C6-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C6-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C6-buffer", "action_params": {"source": "A3", "dest": "C6", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C6", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C6-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 178, "trace_sequence": 178, "timestamp": "2026-09-16T10:39:12+00:00", "logged_at": "2026-09-16T10:39:12.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C6-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C6-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C6-tmb", "action_params": {"source": "A2", "dest": "C6", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C6", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C6-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 179, "trace_sequence": 179, "timestamp": "2026-09-16T10:39:13+00:00", "logged_at": "2026-09-16T10:39:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C6-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C6-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C6-mix", "action_params": {"well": "C6"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C6", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0359051676787858, "rate_uM_per_s": 0.790525921610795, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C6-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 180, "trace_sequence": 180, "timestamp": "2026-09-16T10:39:15+00:00", "logged_at": "2026-09-16T10:39:15.350000+00:00", "source_agent": null, "event_id": "obs-s6-C6-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C6-t3", "wells": {"plate:C6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0359051676787858, "rate_uM_per_s": 0.7905259216107949, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05179525838393929, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:39:12+00:00", "acquired_at": "2026-09-16T10:39:15+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.002964472206040481}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 181, "trace_sequence": 181, "timestamp": "2026-09-16T10:39:18+00:00", "logged_at": "2026-09-16T10:39:18.350000+00:00", "source_agent": null, "event_id": "obs-s6-C6-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C6-t6", "wells": {"plate:C6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0359051676787858, "rate_uM_per_s": 0.7905259216107949, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.10359051676787857, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:39:12+00:00", "acquired_at": "2026-09-16T10:39:18+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005928944412080962}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 182, "trace_sequence": 182, "timestamp": "2026-09-16T10:39:21+00:00", "logged_at": "2026-09-16T10:39:21.350000+00:00", "source_agent": null, "event_id": "obs-s6-C6-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C6-t9", "wells": {"plate:C6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0359051676787858, "rate_uM_per_s": 0.7905259216107949, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.15538577515181787, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:39:12+00:00", "acquired_at": "2026-09-16T10:39:21+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008893416618121444}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 183, "trace_sequence": 183, "timestamp": "2026-09-16T10:39:30+00:00", "logged_at": "2026-09-16T10:39:30.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D6-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D6-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D6-hrp", "action_params": {"source": "A1", "dest": "D6", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D6", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D6-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 184, "trace_sequence": 184, "timestamp": "2026-09-16T10:39:36+00:00", "logged_at": "2026-09-16T10:39:36.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D6-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D6-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D6-buffer", "action_params": {"source": "A3", "dest": "D6", "volume_ul": 152.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D6", "volume_ul": 152.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D6-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 185, "trace_sequence": 185, "timestamp": "2026-09-16T10:39:45+00:00", "logged_at": "2026-09-16T10:39:45.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D6-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D6-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D6-tmb", "action_params": {"source": "A2", "dest": "D6", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D6", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D6-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 186, "trace_sequence": 186, "timestamp": "2026-09-16T10:39:46+00:00", "logged_at": "2026-09-16T10:39:46.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D6-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D6-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D6-mix", "action_params": {"well": "D6"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D6", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0482669366268478, "rate_uM_per_s": 0.799959505972869, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-D6-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 187, "trace_sequence": 187, "timestamp": "2026-09-16T10:39:48+00:00", "logged_at": "2026-09-16T10:39:48.350000+00:00", "source_agent": null, "event_id": "obs-s6-D6-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D6-t3", "wells": {"plate:D6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0482669366268478, "rate_uM_per_s": 0.799959505972869, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05241334683134238, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:39:45+00:00", "acquired_at": "2026-09-16T10:39:48+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0029998481473982584}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 188, "trace_sequence": 188, "timestamp": "2026-09-16T10:39:51+00:00", "logged_at": "2026-09-16T10:39:51.350000+00:00", "source_agent": null, "event_id": "obs-s6-D6-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D6-t6", "wells": {"plate:D6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0482669366268478, "rate_uM_per_s": 0.799959505972869, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.10482669366268477, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:39:45+00:00", "acquired_at": "2026-09-16T10:39:51+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.005999696294796517}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 189, "trace_sequence": 189, "timestamp": "2026-09-16T10:39:54+00:00", "logged_at": "2026-09-16T10:39:54.350000+00:00", "source_agent": null, "event_id": "obs-s6-D6-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D6-t9", "wells": {"plate:D6": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 32.0, "buffer_volume_ul": 152.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0482669366268478, "rate_uM_per_s": 0.799959505972869, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.799959505972869, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.15724004049402715, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:39:45+00:00", "acquired_at": "2026-09-16T10:39:54+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.008999544442194777}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 190, "trace_sequence": 190, "timestamp": "2026-09-16T10:40:04+00:00", "logged_at": "2026-09-16T10:40:04.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B7-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B7-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B7-hrp", "action_params": {"source": "A1", "dest": "B7", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B7", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-B7-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 191, "trace_sequence": 191, "timestamp": "2026-09-16T10:40:13+00:00", "logged_at": "2026-09-16T10:40:13.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B7-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B7-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B7-buffer", "action_params": {"source": "A3", "dest": "B7", "volume_ul": 120.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B7", "volume_ul": 120.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-B7-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 192, "trace_sequence": 192, "timestamp": "2026-09-16T10:40:20+00:00", "logged_at": "2026-09-16T10:40:20.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B7-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-B7-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B7-tmb", "action_params": {"source": "A2", "dest": "B7", "volume_ul": 64.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B7", "volume_ul": 64.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-B7-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 193, "trace_sequence": 193, "timestamp": "2026-09-16T10:40:21+00:00", "logged_at": "2026-09-16T10:40:21.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-B7-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-B7-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-B7-mix", "action_params": {"well": "B7"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:B7", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1211293886075544, "rate_uM_per_s": 0.85556272024386, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-B7-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 194, "trace_sequence": 194, "timestamp": "2026-09-16T10:40:23+00:00", "logged_at": "2026-09-16T10:40:23.350000+00:00", "source_agent": null, "event_id": "obs-s6-B7-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B7-t3", "wells": {"plate:B7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1211293886075544, "rate_uM_per_s": 0.85556272024386, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05605646943037772, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:40:20+00:00", "acquired_at": "2026-09-16T10:40:23+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0016041801004572376}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 195, "trace_sequence": 195, "timestamp": "2026-09-16T10:40:26+00:00", "logged_at": "2026-09-16T10:40:26.350000+00:00", "source_agent": null, "event_id": "obs-s6-B7-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B7-t6", "wells": {"plate:B7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1211293886075544, "rate_uM_per_s": 0.85556272024386, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.11211293886075543, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:40:20+00:00", "acquired_at": "2026-09-16T10:40:26+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.003208360200914475}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 196, "trace_sequence": 196, "timestamp": "2026-09-16T10:40:29+00:00", "logged_at": "2026-09-16T10:40:29.350000+00:00", "source_agent": null, "event_id": "obs-s6-B7-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-B7-t9", "wells": {"plate:B7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1211293886075544, "rate_uM_per_s": 0.85556272024386, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.00943358436207395, "replicate_sign": 1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.16816940829113317, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:40:20+00:00", "acquired_at": "2026-09-16T10:40:29+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.004812540301371713}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 197, "trace_sequence": 197, "timestamp": "2026-09-16T10:40:44+00:00", "logged_at": "2026-09-16T10:40:44.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C7-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C7-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C7-hrp", "action_params": {"source": "A1", "dest": "C7", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C7", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-C7-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 198, "trace_sequence": 198, "timestamp": "2026-09-16T10:40:49+00:00", "logged_at": "2026-09-16T10:40:49.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C7-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C7-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C7-buffer", "action_params": {"source": "A3", "dest": "C7", "volume_ul": 120.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C7", "volume_ul": 120.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-C7-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 199, "trace_sequence": 199, "timestamp": "2026-09-16T10:40:54+00:00", "logged_at": "2026-09-16T10:40:54.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C7-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-C7-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C7-tmb", "action_params": {"source": "A2", "dest": "C7", "volume_ul": 64.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C7", "volume_ul": 64.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-C7-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 200, "trace_sequence": 200, "timestamp": "2026-09-16T10:40:56+00:00", "logged_at": "2026-09-16T10:40:56.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-C7-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-C7-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-C7-mix", "action_params": {"well": "C7"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:C7", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0964058507114307, "rate_uM_per_s": 0.8366955515197121, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-C7-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 201, "trace_sequence": 201, "timestamp": "2026-09-16T10:40:57+00:00", "logged_at": "2026-09-16T10:40:57.350000+00:00", "source_agent": null, "event_id": "obs-s6-C7-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C7-t3", "wells": {"plate:C7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0964058507114307, "rate_uM_per_s": 0.8366955515197119, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05482029253557153, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:40:54+00:00", "acquired_at": "2026-09-16T10:40:57+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0015688041590994598}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 202, "trace_sequence": 202, "timestamp": "2026-09-16T10:41:00+00:00", "logged_at": "2026-09-16T10:41:00.350000+00:00", "source_agent": null, "event_id": "obs-s6-C7-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C7-t6", "wells": {"plate:C7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0964058507114307, "rate_uM_per_s": 0.8366955515197119, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.10964058507114306, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:40:54+00:00", "acquired_at": "2026-09-16T10:41:00+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0031376083181989195}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 203, "trace_sequence": 203, "timestamp": "2026-09-16T10:41:03+00:00", "logged_at": "2026-09-16T10:41:03.350000+00:00", "source_agent": null, "event_id": "obs-s6-C7-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-C7-t9", "wells": {"plate:C7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.0964058507114307, "rate_uM_per_s": 0.8366955515197119, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": -0.00943358436207395, "replicate_sign": -1, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.1644608776067146, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:40:54+00:00", "acquired_at": "2026-09-16T10:41:03+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.00470641247729838}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 204, "trace_sequence": 204, "timestamp": "2026-09-16T10:41:17+00:00", "logged_at": "2026-09-16T10:41:17.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D7-hrp", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D7-hrp", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D7-hrp", "action_params": {"source": "A1", "dest": "D7", "volume_ul": 15.151515151515152, "reagent": "hrp"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D7", "volume_ul": 15.151515151515152, "reagent": "hrp"}}, "result_observation_ids": ["obs-plan-s6-D7-hrp"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 205, "trace_sequence": 205, "timestamp": "2026-09-16T10:41:26+00:00", "logged_at": "2026-09-16T10:41:26.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D7-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D7-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D7-buffer", "action_params": {"source": "A3", "dest": "D7", "volume_ul": 120.84848484848484, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D7", "volume_ul": 120.84848484848484, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-D7-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 206, "trace_sequence": 206, "timestamp": "2026-09-16T10:41:33+00:00", "logged_at": "2026-09-16T10:41:33.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D7-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-D7-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D7-tmb", "action_params": {"source": "A2", "dest": "D7", "volume_ul": 64.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D7", "volume_ul": 64.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-D7-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 207, "trace_sequence": 207, "timestamp": "2026-09-16T10:41:35+00:00", "logged_at": "2026-09-16T10:41:35.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-D7-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-D7-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-D7-mix", "action_params": {"well": "D7"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:D7", "enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1087676196594924, "rate_uM_per_s": 0.8461291358817861, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "result_observation_ids": ["obs-plan-s6-D7-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 208, "trace_sequence": 208, "timestamp": "2026-09-16T10:41:36+00:00", "logged_at": "2026-09-16T10:41:36.350000+00:00", "source_agent": null, "event_id": "obs-s6-D7-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D7-t3", "wells": {"plate:D7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1087676196594924, "rate_uM_per_s": 0.8461291358817858, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.05543838098297462, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:41:33+00:00", "acquired_at": "2026-09-16T10:41:36+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0015864921297783486}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 209, "trace_sequence": 209, "timestamp": "2026-09-16T10:41:39+00:00", "logged_at": "2026-09-16T10:41:39.350000+00:00", "source_agent": null, "event_id": "obs-s6-D7-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D7-t6", "wells": {"plate:D7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1087676196594924, "rate_uM_per_s": 0.8461291358817858, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.11087676196594924, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:41:33+00:00", "acquired_at": "2026-09-16T10:41:39+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.003172984259556697}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 210, "trace_sequence": 210, "timestamp": "2026-09-16T10:41:42+00:00", "logged_at": "2026-09-16T10:41:42.350000+00:00", "source_agent": null, "event_id": "obs-s6-D7-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-D7-t9", "wells": {"plate:D7": {"enzyme_volume_ul": 15.151515151515152, "tmb_volume_ul": 64.0, "buffer_volume_ul": 120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1087676196594924, "rate_uM_per_s": 0.8461291358817858, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true, "A652": 0.16631514294892386, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:41:33+00:00", "acquired_at": "2026-09-16T10:41:42+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0047594763893350454}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 211, "trace_sequence": 211, "timestamp": "2026-09-16T10:41:52+00:00", "logged_at": "2026-09-16T10:41:52.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E1-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E1-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E1-buffer", "action_params": {"source": "A3", "dest": "E1", "volume_ul": 199.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E1", "volume_ul": 199.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E1-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 212, "trace_sequence": 212, "timestamp": "2026-09-16T10:41:56+00:00", "logged_at": "2026-09-16T10:41:56.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E1-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E1-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E1-tmb", "action_params": {"source": "A2", "dest": "E1", "volume_ul": 1.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E1", "volume_ul": 1.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E1-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 213, "trace_sequence": 213, "timestamp": "2026-09-16T10:41:58+00:00", "logged_at": "2026-09-16T10:41:58.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E1-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E1-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E1-mix", "action_params": {"well": "E1"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E1", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0}}, "result_observation_ids": ["obs-plan-s6-E1-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 214, "trace_sequence": 214, "timestamp": "2026-09-16T10:41:59+00:00", "logged_at": "2026-09-16T10:41:59.350000+00:00", "source_agent": null, "event_id": "obs-s6-E1-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E1-t3", "wells": {"plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:41:56+00:00", "acquired_at": "2026-09-16T10:41:59+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 215, "trace_sequence": 215, "timestamp": "2026-09-16T10:42:02+00:00", "logged_at": "2026-09-16T10:42:02.350000+00:00", "source_agent": null, "event_id": "obs-s6-E1-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E1-t6", "wells": {"plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:41:56+00:00", "acquired_at": "2026-09-16T10:42:02+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 216, "trace_sequence": 216, "timestamp": "2026-09-16T10:42:05+00:00", "logged_at": "2026-09-16T10:42:05.350000+00:00", "source_agent": null, "event_id": "obs-s6-E1-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E1-t9", "wells": {"plate:E1": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 1.0, "buffer_volume_ul": 199.0, "total_volume_ul": 200.0, "substrate_uM": 25.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 25.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:41:56+00:00", "acquired_at": "2026-09-16T10:42:05+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 217, "trace_sequence": 217, "timestamp": "2026-09-16T10:42:18+00:00", "logged_at": "2026-09-16T10:42:18.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E2-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E2-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E2-buffer", "action_params": {"source": "A3", "dest": "E2", "volume_ul": 198.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E2", "volume_ul": 198.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E2-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 218, "trace_sequence": 218, "timestamp": "2026-09-16T10:42:27+00:00", "logged_at": "2026-09-16T10:42:27.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E2-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E2-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E2-tmb", "action_params": {"source": "A2", "dest": "E2", "volume_ul": 2.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E2", "volume_ul": 2.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E2-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 219, "trace_sequence": 219, "timestamp": "2026-09-16T10:42:29+00:00", "logged_at": "2026-09-16T10:42:29.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E2-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E2-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E2-mix", "action_params": {"well": "E2"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E2", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0}}, "result_observation_ids": ["obs-plan-s6-E2-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 220, "trace_sequence": 220, "timestamp": "2026-09-16T10:42:30+00:00", "logged_at": "2026-09-16T10:42:30.350000+00:00", "source_agent": null, "event_id": "obs-s6-E2-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E2-t3", "wells": {"plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:42:27+00:00", "acquired_at": "2026-09-16T10:42:30+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 221, "trace_sequence": 221, "timestamp": "2026-09-16T10:42:33+00:00", "logged_at": "2026-09-16T10:42:33.350000+00:00", "source_agent": null, "event_id": "obs-s6-E2-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E2-t6", "wells": {"plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:42:27+00:00", "acquired_at": "2026-09-16T10:42:33+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 222, "trace_sequence": 222, "timestamp": "2026-09-16T10:42:36+00:00", "logged_at": "2026-09-16T10:42:36.350000+00:00", "source_agent": null, "event_id": "obs-s6-E2-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E2-t9", "wells": {"plate:E2": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 2.0, "buffer_volume_ul": 198.0, "total_volume_ul": 200.0, "substrate_uM": 50.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 50.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:42:27+00:00", "acquired_at": "2026-09-16T10:42:36+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 223, "trace_sequence": 223, "timestamp": "2026-09-16T10:42:48+00:00", "logged_at": "2026-09-16T10:42:48.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E3-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E3-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E3-buffer", "action_params": {"source": "A3", "dest": "E3", "volume_ul": 196.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E3", "volume_ul": 196.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E3-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 224, "trace_sequence": 224, "timestamp": "2026-09-16T10:42:57+00:00", "logged_at": "2026-09-16T10:42:57.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E3-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E3-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E3-tmb", "action_params": {"source": "A2", "dest": "E3", "volume_ul": 4.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E3", "volume_ul": 4.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E3-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 225, "trace_sequence": 225, "timestamp": "2026-09-16T10:42:58+00:00", "logged_at": "2026-09-16T10:42:58.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E3-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E3-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E3-mix", "action_params": {"well": "E3"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E3", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0}}, "result_observation_ids": ["obs-plan-s6-E3-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 226, "trace_sequence": 226, "timestamp": "2026-09-16T10:43:00+00:00", "logged_at": "2026-09-16T10:43:00.350000+00:00", "source_agent": null, "event_id": "obs-s6-E3-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E3-t3", "wells": {"plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:42:57+00:00", "acquired_at": "2026-09-16T10:43:00+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 227, "trace_sequence": 227, "timestamp": "2026-09-16T10:43:03+00:00", "logged_at": "2026-09-16T10:43:03.350000+00:00", "source_agent": null, "event_id": "obs-s6-E3-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E3-t6", "wells": {"plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:42:57+00:00", "acquired_at": "2026-09-16T10:43:03+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 228, "trace_sequence": 228, "timestamp": "2026-09-16T10:43:06+00:00", "logged_at": "2026-09-16T10:43:06.350000+00:00", "source_agent": null, "event_id": "obs-s6-E3-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E3-t9", "wells": {"plate:E3": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 4.0, "buffer_volume_ul": 196.0, "total_volume_ul": 200.0, "substrate_uM": 100.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 100.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:42:57+00:00", "acquired_at": "2026-09-16T10:43:06+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 229, "trace_sequence": 229, "timestamp": "2026-09-16T10:43:15+00:00", "logged_at": "2026-09-16T10:43:15.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E4-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E4-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E4-buffer", "action_params": {"source": "A3", "dest": "E4", "volume_ul": 192.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E4", "volume_ul": 192.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E4-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 230, "trace_sequence": 230, "timestamp": "2026-09-16T10:43:22+00:00", "logged_at": "2026-09-16T10:43:22.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E4-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E4-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E4-tmb", "action_params": {"source": "A2", "dest": "E4", "volume_ul": 8.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E4", "volume_ul": 8.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E4-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 231, "trace_sequence": 231, "timestamp": "2026-09-16T10:43:23+00:00", "logged_at": "2026-09-16T10:43:23.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E4-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E4-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E4-mix", "action_params": {"well": "E4"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E4", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0}}, "result_observation_ids": ["obs-plan-s6-E4-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 232, "trace_sequence": 232, "timestamp": "2026-09-16T10:43:25+00:00", "logged_at": "2026-09-16T10:43:25.350000+00:00", "source_agent": null, "event_id": "obs-s6-E4-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E4-t3", "wells": {"plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:43:22+00:00", "acquired_at": "2026-09-16T10:43:25+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 233, "trace_sequence": 233, "timestamp": "2026-09-16T10:43:28+00:00", "logged_at": "2026-09-16T10:43:28.350000+00:00", "source_agent": null, "event_id": "obs-s6-E4-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E4-t6", "wells": {"plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:43:22+00:00", "acquired_at": "2026-09-16T10:43:28+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 234, "trace_sequence": 234, "timestamp": "2026-09-16T10:43:31+00:00", "logged_at": "2026-09-16T10:43:31.350000+00:00", "source_agent": null, "event_id": "obs-s6-E4-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E4-t9", "wells": {"plate:E4": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 8.0, "buffer_volume_ul": 192.0, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 200.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:43:22+00:00", "acquired_at": "2026-09-16T10:43:31+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 235, "trace_sequence": 235, "timestamp": "2026-09-16T10:43:41+00:00", "logged_at": "2026-09-16T10:43:41.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E5-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E5-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E5-buffer", "action_params": {"source": "A3", "dest": "E5", "volume_ul": 184.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E5", "volume_ul": 184.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E5-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 236, "trace_sequence": 236, "timestamp": "2026-09-16T10:43:45+00:00", "logged_at": "2026-09-16T10:43:45.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E5-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E5-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E5-tmb", "action_params": {"source": "A2", "dest": "E5", "volume_ul": 16.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E5", "volume_ul": 16.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E5-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 237, "trace_sequence": 237, "timestamp": "2026-09-16T10:43:47+00:00", "logged_at": "2026-09-16T10:43:47.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E5-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E5-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E5-mix", "action_params": {"well": "E5"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E5", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0}}, "result_observation_ids": ["obs-plan-s6-E5-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 238, "trace_sequence": 238, "timestamp": "2026-09-16T10:43:48+00:00", "logged_at": "2026-09-16T10:43:48.350000+00:00", "source_agent": null, "event_id": "obs-s6-E5-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E5-t3", "wells": {"plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:43:45+00:00", "acquired_at": "2026-09-16T10:43:48+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 239, "trace_sequence": 239, "timestamp": "2026-09-16T10:43:51+00:00", "logged_at": "2026-09-16T10:43:51.350000+00:00", "source_agent": null, "event_id": "obs-s6-E5-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E5-t6", "wells": {"plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:43:45+00:00", "acquired_at": "2026-09-16T10:43:51+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 240, "trace_sequence": 240, "timestamp": "2026-09-16T10:43:54+00:00", "logged_at": "2026-09-16T10:43:54.350000+00:00", "source_agent": null, "event_id": "obs-s6-E5-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E5-t9", "wells": {"plate:E5": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 16.0, "buffer_volume_ul": 184.0, "total_volume_ul": 200.0, "substrate_uM": 400.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 400.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:43:45+00:00", "acquired_at": "2026-09-16T10:43:54+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 241, "trace_sequence": 241, "timestamp": "2026-09-16T10:44:05+00:00", "logged_at": "2026-09-16T10:44:05.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E6-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E6-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E6-buffer", "action_params": {"source": "A3", "dest": "E6", "volume_ul": 168.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E6", "volume_ul": 168.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E6-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 242, "trace_sequence": 242, "timestamp": "2026-09-16T10:44:09+00:00", "logged_at": "2026-09-16T10:44:09.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E6-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E6-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E6-tmb", "action_params": {"source": "A2", "dest": "E6", "volume_ul": 32.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E6", "volume_ul": 32.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E6-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 243, "trace_sequence": 243, "timestamp": "2026-09-16T10:44:10+00:00", "logged_at": "2026-09-16T10:44:10.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E6-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E6-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E6-mix", "action_params": {"well": "E6"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E6", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 32.0, "buffer_volume_ul": 168.0, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 800.0}}, "result_observation_ids": ["obs-plan-s6-E6-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 244, "trace_sequence": 244, "timestamp": "2026-09-16T10:44:12+00:00", "logged_at": "2026-09-16T10:44:12.350000+00:00", "source_agent": null, "event_id": "obs-s6-E6-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E6-t3", "wells": {"plate:E6": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 32.0, "buffer_volume_ul": 168.0, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 800.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:44:09+00:00", "acquired_at": "2026-09-16T10:44:12+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 245, "trace_sequence": 245, "timestamp": "2026-09-16T10:44:15+00:00", "logged_at": "2026-09-16T10:44:15.350000+00:00", "source_agent": null, "event_id": "obs-s6-E6-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E6-t6", "wells": {"plate:E6": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 32.0, "buffer_volume_ul": 168.0, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 800.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:44:09+00:00", "acquired_at": "2026-09-16T10:44:15+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 246, "trace_sequence": 246, "timestamp": "2026-09-16T10:44:18+00:00", "logged_at": "2026-09-16T10:44:18.350000+00:00", "source_agent": null, "event_id": "obs-s6-E6-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E6-t9", "wells": {"plate:E6": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 32.0, "buffer_volume_ul": 168.0, "total_volume_ul": 200.0, "substrate_uM": 800.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 800.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:44:09+00:00", "acquired_at": "2026-09-16T10:44:18+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 247, "trace_sequence": 247, "timestamp": "2026-09-16T10:44:32+00:00", "logged_at": "2026-09-16T10:44:32.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E7-buffer", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E7-buffer", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E7-buffer", "action_params": {"source": "A3", "dest": "E7", "volume_ul": 136.0, "reagent": "buffer"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E7", "volume_ul": 136.0, "reagent": "buffer"}}, "result_observation_ids": ["obs-plan-s6-E7-buffer"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 248, "trace_sequence": 248, "timestamp": "2026-09-16T10:44:39+00:00", "logged_at": "2026-09-16T10:44:39.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E7-tmb", "event_type": "tool_call", "tool_name": "transfer", "tool_call_id": "plan-s6-E7-tmb", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E7-tmb", "action_params": {"source": "A2", "dest": "E7", "volume_ul": 64.0, "reagent": "tmb"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E7", "volume_ul": 64.0, "reagent": "tmb"}}, "result_observation_ids": ["obs-plan-s6-E7-tmb"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 249, "trace_sequence": 249, "timestamp": "2026-09-16T10:44:40+00:00", "logged_at": "2026-09-16T10:44:40.350000+00:00", "source_agent": "actor", "event_id": "act-plan-s6-E7-mix", "event_type": "tool_call", "tool_name": "mix_well", "tool_call_id": "plan-s6-E7-mix", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "plan-s6-E7-mix", "action_params": {"well": "E7"}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"well": "plate:E7", "enzyme_volume_ul": 0.0, "tmb_volume_ul": 64.0, "buffer_volume_ul": 136.0, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 1600.0}}, "result_observation_ids": ["obs-plan-s6-E7-mix"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 250, "trace_sequence": 250, "timestamp": "2026-09-16T10:44:42+00:00", "logged_at": "2026-09-16T10:44:42.350000+00:00", "source_agent": null, "event_id": "obs-s6-E7-t3", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E7-t3", "wells": {"plate:E7": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 64.0, "buffer_volume_ul": 136.0, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 1600.0, "A652": 0.0, "elapsed_s": 3.0, "reaction_started_at": "2026-09-16T10:44:39+00:00", "acquired_at": "2026-09-16T10:44:42+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 251, "trace_sequence": 251, "timestamp": "2026-09-16T10:44:45+00:00", "logged_at": "2026-09-16T10:44:45.350000+00:00", "source_agent": null, "event_id": "obs-s6-E7-t6", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E7-t6", "wells": {"plate:E7": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 64.0, "buffer_volume_ul": 136.0, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 1600.0, "A652": 0.0, "elapsed_s": 6.0, "reaction_started_at": "2026-09-16T10:44:39+00:00", "acquired_at": "2026-09-16T10:44:45+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 252, "trace_sequence": 252, "timestamp": "2026-09-16T10:44:48+00:00", "logged_at": "2026-09-16T10:44:48.350000+00:00", "source_agent": null, "event_id": "obs-s6-E7-t9", "event_type": "observation", "observation_type": "public_measurement", "payload": {"observation_id": "s6-E7-t9", "wells": {"plate:E7": {"enzyme_volume_ul": 0.0, "tmb_volume_ul": 64.0, "buffer_volume_ul": 136.0, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.0, "enzyme_concentration_ng_ml": 0.0, "enzyme_concentration_M": 0.0, "rate_A652_per_min": 0.0, "rate_uM_per_s": 0.0, "series": "blank", "used_for_fit": false, "initial_rate_eligible": false, "blank_for_substrate_uM": 1600.0, "A652": 0.0, "elapsed_s": 9.0, "reaction_started_at": "2026-09-16T10:44:39+00:00", "acquired_at": "2026-09-16T10:44:48+00:00", "acid_stopped": false, "substrate_conversion_fraction": 0.0}}, "note": "Same-well absorbance at 3, 6, and 9 s after TMB was dispensed. No acid stop."}} | |
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120.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 1600.0, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_M": 2.2727272727272728e-10, "rate_A652_per_min": 1.1087676196594924, "rate_uM_per_s": 0.8461291358817861, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 15.151515151515152, "rate_uM_per_s_mean": 0.8461291358817861, "rate_residual_uM_per_s": 0.0, "replicate_sign": 0, "series": "mm", "used_for_fit": true, "initial_rate_eligible": true}}, "note": "Prepared composition of the 21-well substrate series. Final HRP is 10 ng/mL in 200 uL. Timed absorbance is in the read events."}} | |
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"tmb_volume_ul": 8.0, "buffer_volume_ul": 131.39393939393938, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 0.9090909090909091, "enzyme_concentration_ng_ml": 40.0, "enzyme_concentration_M": 9.090909090909091e-10, "rate_A652_per_min": 2.369153630262355, "rate_uM_per_s": 1.8079621720561314, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 60.60606060606061, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}, "plate:A8": {"enzyme_volume_ul": 121.21212121212122, "tmb_volume_ul": 8.0, "buffer_volume_ul": 70.78787878787878, "total_volume_ul": 200.0, "substrate_uM": 200.0, "enzyme_concentration_nM": 1.8181818181818181, "enzyme_concentration_ng_ml": 80.0, "enzyme_concentration_M": 1.8181818181818182e-09, "rate_A652_per_min": 3.5537304453935326, "rate_uM_per_s": 2.711943258084197, "hrp_source": "A1", "hrp_source_labware": "reservoir", "dilution_factor": 1, "neat_enzyme_ul": 121.21212121212122, "series": "hrp_working_range", "used_for_fit": false, "initial_rate_eligible": true}}, "note": "HRP working-range titration at 200 uM TMB. The linear window is the HRP concentration, not the TMB span."}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 255, "trace_sequence": 255, "timestamp": "2026-09-16T10:45:24+00:00", "logged_at": "2026-09-16T10:45:24.350000+00:00", "source_agent": "planner", "event_id": "msg-0255", "event_type": "recovery_decision", "rationale": "D3 is much faster than the other wells prepared at the same nominal substrate. It looks as if that one well was contaminated. I do not know what reached it. I exclude D3 and keep the other 20 wells. I do not repeat the assay.", "message": "D3 is much faster than the other wells prepared at the same nominal substrate. It looks as if that one well was contaminated. I do not know what reached it. I exclude D3 and keep the other 20 wells. I do not repeat the assay."} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 256, "trace_sequence": 256, "timestamp": "2026-09-16T10:45:36+00:00", "logged_at": "2026-09-16T10:45:36.350000+00:00", "source_agent": null, "event_id": "appr-appr-s6-exclude", "event_type": "proceed_signal", "plan_id": "plan-s6", "revision_id": "rev_0", "approval_id": "appr-s6-exclude", "approved_action_ids": ["s6-exclude"], "message": "Approved against the public deck and the stated instruction."} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 257, "trace_sequence": 257, "timestamp": "2026-09-16T10:45:48+00:00", "logged_at": "2026-09-16T10:45:48.350000+00:00", "source_agent": "actor", "event_id": "act-s6-exclude", "event_type": "tool_call", "tool_name": "exclude_well", "tool_call_id": "s6-exclude", "plan_id": "plan-s6", "revision_id": "rev_0", "executed": true, "params": {"action_id": "s6-exclude", "action_params": {"wells": ["D3"]}, "approved_action_association": "associated"}, "result": {"ok": true, "error": "", "observation": {"excluded": ["D3"]}}, "result_observation_ids": ["obs-s6-exclude"], "state_delta": {"execution_disposition": "dispatched"}} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 258, "trace_sequence": 258, "timestamp": "2026-09-16T10:46:00+00:00", "logged_at": "2026-09-16T10:46:00.350000+00:00", "source_agent": "planner", "event_id": "msg-0258", "event_type": "recovery_decision", "rationale": "Reported KM = 98 uM. kcat = Vmax/[E] = 3951 /s at 10 ng/mL (0.2273 nM) HRP. Reported kcat uses the prepared-well concentration, 10 ng/mL. Vmax is 0.8980 uM/s, converted from 1.1767 A652/min with epsilon 39000 /M/cm and path 0.56 cm. That path is 0.28 cm times the well volume divided by 100 uL, which is 0.56 cm at 200 uL. The linear HRP working range is 0.5-10 ng/mL.", "message": "Reported KM = 98 uM. kcat = Vmax/[E] = 3951 /s at 10 ng/mL (0.2273 nM) HRP. Reported kcat uses the prepared-well concentration, 10 ng/mL. Vmax is 0.8980 uM/s, converted from 1.1767 A652/min with epsilon 39000 /M/cm and path 0.56 cm. That path is 0.28 cm times the well volume divided by 100 uL, which is 0.56 cm at 200 uL. The linear HRP working range is 0.5-10 ng/mL."} | |
| {"record_kind": "event", "run_id": "pearl-manuscript-s6-20260916", "seq": 259, "trace_sequence": 259, "timestamp": "2026-09-16T10:46:12+00:00", "logged_at": "2026-09-16T10:46:12.350000+00:00", "source_agent": "planner", "event_id": "done-S6", "event_type": "completion_decision", "plan_id": "plan-s6", "revision_id": "rev_0", "adopted": {"summary": "Kinetic characterization after excluding one contaminated well.", "KM_uM": 98.0, "kcat_per_s": 3951.0, "Vmax_M_per_s": 8.979545454545455e-07, "Vmax_uM_per_s": 0.8979545454545454, "Vmax_A652_per_min": 1.1766796363636363, "enzyme_concentration_M": 2.2727272727272728e-10, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "basis": "kcat_per_s = Vmax_M_per_s / enzyme_concentration_M", "read_mode": "same_well", "acid_stop": false, "enzyme_stock": "3 nM nominal, 2000 uL", "working_range_analyte": "HRP", "linear_working_range_hrp_ng_ml": [0.5, 10.0], "linear_working_range_hrp_nM": [0.011363636363636364, 0.22727272727272727], "preparation_id": "prep-s6-001", "n_wells": 21, "excluded_wells": ["D3"], "spike": {"well": "D3", "extra_tmb_ul": 5, "disclosed_to_agent": false, "selection_population": "substrate_series", "selection_limit": 15, "substrate_series_index": 9, "titration_included": false}, "repeated_assay": false}, "discarded": ["D3"], "message": "Kinetic characterization after excluding one contaminated well."} | |
| {"record_kind": "trial", "run_id": "pearl-manuscript-s6-20260916", "seq": 260, "trace_sequence": 260, "timestamp": "2026-09-16T10:46:24+00:00", "logged_at": "2026-09-16T10:46:24.350000+00:00", "source_agent": null, "scenario_id": "S6", "termination": "completed", "not_wet_lab": false, "adopted": {"summary": "Kinetic characterization after excluding one contaminated well.", "KM_uM": 98.0, "kcat_per_s": 3951.0, "Vmax_M_per_s": 8.979545454545455e-07, "Vmax_uM_per_s": 0.8979545454545454, "Vmax_A652_per_min": 1.1766796363636363, "enzyme_concentration_M": 2.2727272727272728e-10, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "basis": "kcat_per_s = Vmax_M_per_s / enzyme_concentration_M", "read_mode": "same_well", "acid_stop": false, "enzyme_stock": "3 nM nominal, 2000 uL", "working_range_analyte": "HRP", "linear_working_range_hrp_ng_ml": [0.5, 10.0], "linear_working_range_hrp_nM": [0.011363636363636364, 0.22727272727272727], "preparation_id": "prep-s6-001", "n_wells": 21, "excluded_wells": ["D3"], "spike": {"well": "D3", "extra_tmb_ul": 5, "disclosed_to_agent": false, "selection_population": "substrate_series", "selection_limit": 15, "substrate_series_index": 9, "titration_included": false}, "repeated_assay": false}, "discarded": ["D3"], "commentary": "The agent suspected that one well, D3, was contaminated and did not identify a cause. The assay was not repeated. Reported KM = 98 uM and kcat = Vmax/[E] = 3951 /s.", "analysis_report": {"KM_uM": 98.0, "kcat_per_s": 3951.0, "kcat_calculation": {"equation": "kcat_per_s = Vmax_M_per_s / enzyme_concentration_M", "absorbance_conversion": "Vmax_M_per_s = Vmax_A652_per_min / (epsilon_per_M_cm * path_cm * 60)", "concentration_conversion": "ng/mL = nM * hrp_mw_g_per_mol / 1000", "KM_uM": 98.0, "kcat_per_s": 3951.0, "physical_kcat_per_s": 3951.0, "replicate_delta_uM_per_s": 0.00943358436207395, "fit_KM_uM": 98.0, "fit_Vmax_uM_per_s": 0.8979545454545454, "fit_r2": 0.9989171220745329, "Vmax_M_per_s": 8.979545454545455e-07, "Vmax_uM_per_s": 0.8979545454545454, "Vmax_A652_per_min": 1.1766796363636363, "epsilon_per_M_cm": 39000.0, "path_cm": 0.56, "wavelength_nm": 652, "hrp_mw_g_per_mol": 44000.0, "enzyme_stock_nM": 3.0, "enzyme_stock_volume_ul": 2000.0, "enzyme_stock_ng_ml": 132.0, "enzyme_volume_ul": 15.151515151515152, "neat_enzyme_ul": 15.151515151515152, "enzyme_source_nM": 3.0, "enzyme_source_well": "A1", "enzyme_source_labware": "reservoir", "dilution_factor": 1, "total_volume_ul": 200.0, "enzyme_concentration_M": 2.2727272727272728e-10, "enzyme_concentration_nM": 0.22727272727272727, "enzyme_concentration_ng_ml": 10.0, "physical_enzyme_concentration_M": 2.2727272727272728e-10, "physical_enzyme_concentration_nM": 0.22727272727272727, "physical_enzyme_concentration_ng_ml": 10.0, "enzyme_concentration_ng_ml_from_label": 10.0, "kcat_basis": "prepared_well", "apparent_kcat_per_s_on_label": 3951.0, "kcat_basis_note": "Reported kcat divides Vmax by the prepared-well enzyme concentration. The labelled stock and the dispense volumes produce that concentration."}, "working_range": {"analyte": "HRP", "criterion": "initial rate proportional to final HRP concentration", "fixed_substrate_uM": 200.0, "linear_ng_ml": [0.5, 10.0], "linear_nM": [0.011363636363636364, 0.22727272727272727], "assay_enzyme_ng_ml": 10.0, "assay_enzyme_nM": 0.22727272727272727, "points": [{"hrp_ng_ml": 0.5, "hrp_nM": 0.011363636363636364, "enzyme_volume_ul": 1.5151515151515151, "neat_enzyme_ul": 0.7575757575757576, "hrp_source": "H12", "hrp_source_labware": "plate", "enzyme_source_nM": 1.5, "dilution_factor": 2, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.03948589383770591, "rate_uM_per_s": 0.03013270286760219, "in_linear_range": true}, {"hrp_ng_ml": 1.0, "hrp_nM": 0.022727272727272728, "enzyme_volume_ul": 1.5151515151515151, "neat_enzyme_ul": 1.5151515151515151, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 190.4848484848485, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.07897178767541183, "rate_uM_per_s": 0.06026540573520438, "in_linear_range": true}, {"hrp_ng_ml": 2.0, "hrp_nM": 0.045454545454545456, "enzyme_volume_ul": 3.0303030303030303, "neat_enzyme_ul": 3.0303030303030303, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 188.96969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.15794357535082365, "rate_uM_per_s": 0.12053081147040876, "in_linear_range": true}, {"hrp_ng_ml": 5.0, "hrp_nM": 0.11363636363636363, "enzyme_volume_ul": 7.575757575757576, "neat_enzyme_ul": 7.575757575757576, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 184.42424242424244, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.39485893837705915, "rate_uM_per_s": 0.3013270286760219, "in_linear_range": true}, {"hrp_ng_ml": 10.0, "hrp_nM": 0.22727272727272727, "enzyme_volume_ul": 15.151515151515152, "neat_enzyme_ul": 15.151515151515152, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 176.84848484848484, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 0.7897178767541183, "rate_uM_per_s": 0.6026540573520438, "in_linear_range": true}, {"hrp_ng_ml": 20.0, "hrp_nM": 0.45454545454545453, "enzyme_volume_ul": 30.303030303030305, "neat_enzyme_ul": 30.303030303030305, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 161.6969696969697, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 1.4214921781574128, "rate_uM_per_s": 1.0847773032336787, "in_linear_range": false}, {"hrp_ng_ml": 40.0, "hrp_nM": 0.9090909090909091, "enzyme_volume_ul": 60.60606060606061, "neat_enzyme_ul": 60.60606060606061, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 131.39393939393938, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 2.369153630262355, "rate_uM_per_s": 1.8079621720561314, "in_linear_range": false}, {"hrp_ng_ml": 80.0, "hrp_nM": 1.8181818181818181, "enzyme_volume_ul": 121.21212121212122, "neat_enzyme_ul": 121.21212121212122, "hrp_source": "A1", "hrp_source_labware": "reservoir", "enzyme_source_nM": 3.0, "dilution_factor": 1, "tmb_volume_ul": 8.0, "buffer_volume_ul": 70.78787878787878, "total_volume_ul": 200.0, "substrate_uM": 200.0, "rate_A652_per_min": 3.5537304453935326, "rate_uM_per_s": 2.711943258084197, "in_linear_range": false}]}}} | |