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  1. RNA-seq/1-HEK293T-1_S1_salmon/aux_info/ambig_info.tsv +0 -0
  2. RNA-seq/1-HEK293T-1_S1_salmon/aux_info/meta_info.json +44 -0
  3. RNA-seq/1-HEK293T-1_S1_salmon/cmd_info.json +17 -0
  4. RNA-seq/1-HEK293T-1_S1_salmon/libParams/flenDist.txt +2 -0
  5. RNA-seq/1-HEK293T-1_S1_salmon/lib_format_counts.json +21 -0
  6. RNA-seq/1-HEK293T-1_S1_salmon/logs/salmon_quant.log +61 -0
  7. RNA-seq/10-HS707A_S10_salmon/aux_info/ambig_info.tsv +0 -0
  8. RNA-seq/10-HS707A_S10_salmon/aux_info/meta_info.json +44 -0
  9. RNA-seq/10-HS707A_S10_salmon/libParams/flenDist.txt +2 -0
  10. RNA-seq/10-HS707A_S10_salmon/logs/salmon_quant.log +53 -0
  11. RNA-seq/11-MCF7_S11_L001_R1_001.fastq.gz +3 -0
  12. RNA-seq/11-MCF7_S11_salmon/aux_info/ambig_info.tsv +0 -0
  13. RNA-seq/11-MCF7_S11_salmon/aux_info/meta_info.json +44 -0
  14. RNA-seq/11-MCF7_S11_salmon/cmd_info.json +17 -0
  15. RNA-seq/11-MCF7_S11_salmon/libParams/flenDist.txt +2 -0
  16. RNA-seq/11-MCF7_S11_salmon/lib_format_counts.json +21 -0
  17. RNA-seq/11-MCF7_S11_salmon/logs/salmon_quant.log +56 -0
  18. RNA-seq/12-ZR-75-1_S12_salmon/aux_info/ambig_info.tsv +0 -0
  19. RNA-seq/12-ZR-75-1_S12_salmon/aux_info/meta_info.json +44 -0
  20. RNA-seq/12-ZR-75-1_S12_salmon/cmd_info.json +17 -0
  21. RNA-seq/12-ZR-75-1_S12_salmon/libParams/flenDist.txt +2 -0
  22. RNA-seq/12-ZR-75-1_S12_salmon/lib_format_counts.json +21 -0
  23. RNA-seq/12-ZR-75-1_S12_salmon/logs/salmon_quant.log +58 -0
  24. RNA-seq/13-A549_S13_salmon/aux_info/ambig_info.tsv +0 -0
  25. RNA-seq/13-A549_S13_salmon/aux_info/meta_info.json +44 -0
  26. RNA-seq/13-A549_S13_salmon/cmd_info.json +17 -0
  27. RNA-seq/13-A549_S13_salmon/lib_format_counts.json +21 -0
  28. RNA-seq/14-BxPC3_S14_L001_R1_001.fastq.gz +3 -0
  29. RNA-seq/14-BxPC3_S14_salmon/aux_info/ambig_info.tsv +0 -0
  30. RNA-seq/14-BxPC3_S14_salmon/aux_info/meta_info.json +44 -0
  31. RNA-seq/14-BxPC3_S14_salmon/cmd_info.json +17 -0
  32. RNA-seq/14-BxPC3_S14_salmon/libParams/flenDist.txt +2 -0
  33. RNA-seq/14-BxPC3_S14_salmon/lib_format_counts.json +21 -0
  34. RNA-seq/14-BxPC3_S14_salmon/logs/salmon_quant.log +52 -0
  35. RNA-seq/15-Colo320_S15_salmon/aux_info/ambig_info.tsv +0 -0
  36. RNA-seq/15-Colo320_S15_salmon/aux_info/meta_info.json +44 -0
  37. RNA-seq/15-Colo320_S15_salmon/cmd_info.json +17 -0
  38. RNA-seq/15-Colo320_S15_salmon/libParams/flenDist.txt +2 -0
  39. RNA-seq/15-Colo320_S15_salmon/lib_format_counts.json +21 -0
  40. RNA-seq/15-Colo320_S15_salmon/logs/salmon_quant.log +56 -0
  41. RNA-seq/16-HCC44_S16_L001_R1_001.fastq.gz +3 -0
  42. RNA-seq/16-HCC44_S16_salmon/aux_info/ambig_info.tsv +0 -0
  43. RNA-seq/16-HCC44_S16_salmon/aux_info/meta_info.json +44 -0
  44. RNA-seq/16-HCC44_S16_salmon/cmd_info.json +17 -0
  45. RNA-seq/16-HCC44_S16_salmon/libParams/flenDist.txt +2 -0
  46. RNA-seq/16-HCC44_S16_salmon/lib_format_counts.json +21 -0
  47. RNA-seq/16-HCC44_S16_salmon/logs/salmon_quant.log +54 -0
  48. RNA-seq/17-HEK293T-3_S17_salmon/aux_info/ambig_info.tsv +0 -0
  49. RNA-seq/17-HEK293T-3_S17_salmon/aux_info/meta_info.json +44 -0
  50. RNA-seq/17-HEK293T-3_S17_salmon/cmd_info.json +17 -0
RNA-seq/1-HEK293T-1_S1_salmon/aux_info/ambig_info.tsv ADDED
The diff for this file is too large to render. See raw diff
 
RNA-seq/1-HEK293T-1_S1_salmon/aux_info/meta_info.json ADDED
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+ "num_bootstraps": 0,
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+ "num_processed": 19791519,
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+ "num_mapped": 10316668,
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+ "num_decoy_fragments": 0,
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+ "num_dovetail_fragments": 71901,
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+ "num_fragments_filtered_vm": 453017,
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+ "num_alignments_below_threshold_for_mapped_fragments_vm": 4172252,
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+ "percent_mapped": 52.1267114464534,
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+ "call": "quant",
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+ "start_time": "Wed Nov 4 13:02:51 2020",
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+ "end_time": "Wed Nov 4 13:04:56 2020"
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+ }
RNA-seq/1-HEK293T-1_S1_salmon/cmd_info.json ADDED
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+ "validateMappings": [],
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+ "index": "gencode.v35.transcripts.salmon.index",
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+ "libType": "ISR",
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+ "mates1": [
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+ "1-HEK293T-1_S1_L001_R1_001.fastq.gz",
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+ "1-HEK293T-1_S1_L002_R1_001.fastq.gz"
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+ ],
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+ "mates2": [
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+ "1-HEK293T-1_S1_L001_R2_001.fastq.gz",
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+ "1-HEK293T-1_S1_L002_R2_001.fastq.gz"
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+ ],
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+ "threads": "12",
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+ "output": "1-HEK293T-1_S1_salmon",
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+ "auxDir": "aux_info"
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+ }
RNA-seq/1-HEK293T-1_S1_salmon/libParams/flenDist.txt ADDED
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2
+
RNA-seq/1-HEK293T-1_S1_salmon/lib_format_counts.json ADDED
@@ -0,0 +1,21 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ {
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+ "read_files": "[ [ 1-HEK293T-1_S1_L001_R1_001.fastq.gz, 1-HEK293T-1_S1_L001_R2_001.fastq.gz], [ 1-HEK293T-1_S1_L002_R1_001.fastq.gz, 1-HEK293T-1_S1_L002_R2_001.fastq.gz] ]",
3
+ "expected_format": "ISR",
4
+ "compatible_fragment_ratio": 1.0,
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+ "num_compatible_fragments": 10316668,
6
+ "num_assigned_fragments": 10316668,
7
+ "num_frags_with_consistent_mappings": 8568749,
8
+ "num_frags_with_inconsistent_or_orphan_mappings": 1747919,
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+ "MSF": 0,
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+ "OSF": 0,
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+ "ISF": 0,
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+ "MSR": 0,
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+ "OSR": 0,
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+ "ISR": 8568749,
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+ "SF": 685006,
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+ "SR": 1062913,
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+ "MU": 0,
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+ "OU": 0,
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+ "IU": 0,
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+ "U": 0
21
+ }
RNA-seq/1-HEK293T-1_S1_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,61 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 13:02:51.086] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 13:02:51.086] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 13:02:51.086] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 13:02:51.086] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 13:02:51.086] [jointLog] [info] parsing read library format
6
+ [2020-11-04 13:02:51.086] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 13:02:51.165] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 13:02:51.167] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 13:02:58.898] [jointLog] [info] done
10
+ [2020-11-04 13:02:58.898] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 13:03:48.024] [jointLog] [info] Thread saw mini-batch with a maximum of 3.90% zero probability fragments
12
+ [2020-11-04 13:03:48.039] [jointLog] [info] Thread saw mini-batch with a maximum of 4.08% zero probability fragments
13
+ [2020-11-04 13:03:48.059] [jointLog] [info] Thread saw mini-batch with a maximum of 3.78% zero probability fragments
14
+ [2020-11-04 13:03:48.088] [jointLog] [info] Thread saw mini-batch with a maximum of 3.92% zero probability fragments
15
+ [2020-11-04 13:03:48.098] [jointLog] [info] Thread saw mini-batch with a maximum of 3.80% zero probability fragments
16
+ [2020-11-04 13:03:48.101] [jointLog] [info] Thread saw mini-batch with a maximum of 3.86% zero probability fragments
17
+ [2020-11-04 13:03:48.103] [jointLog] [info] Thread saw mini-batch with a maximum of 3.82% zero probability fragments
18
+ [2020-11-04 13:03:48.111] [jointLog] [info] Thread saw mini-batch with a maximum of 3.76% zero probability fragments
19
+ [2020-11-04 13:03:48.123] [jointLog] [info] Thread saw mini-batch with a maximum of 3.92% zero probability fragments
20
+ [2020-11-04 13:03:48.134] [jointLog] [info] Thread saw mini-batch with a maximum of 4.04% zero probability fragments
21
+ [2020-11-04 13:03:48.142] [jointLog] [info] Thread saw mini-batch with a maximum of 3.78% zero probability fragments
22
+ [2020-11-04 13:03:48.157] [jointLog] [info] Thread saw mini-batch with a maximum of 3.78% zero probability fragments
23
+ [2020-11-04 13:03:48.785] [fileLog] [info]
24
+ At end of round 0
25
+ ==================
26
+ Observed 19791519 total fragments (19791519 in most recent round)
27
+
28
+ [2020-11-04 13:03:48.785] [jointLog] [info] Computed 758,085 rich equivalence classes for further processing
29
+ [2020-11-04 13:03:48.785] [jointLog] [info] Counted 10,316,668 total reads in the equivalence classes
30
+ [2020-11-04 13:03:48.803] [jointLog] [info] Number of mappings discarded because of alignment score : 4,172,252
31
+ [2020-11-04 13:03:48.803] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 453,017
32
+ [2020-11-04 13:03:48.803] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
33
+ [2020-11-04 13:03:48.803] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 71,901
34
+ [2020-11-04 13:03:48.803] [jointLog] [info] Mapping rate = 52.1267%
35
+
36
+ [2020-11-04 13:03:48.803] [jointLog] [info] finished quantifyLibrary()
37
+ [2020-11-04 13:03:48.805] [jointLog] [info] Starting optimizer
38
+ [2020-11-04 13:04:01.596] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 13:04:01.630] [jointLog] [info] iteration = 0 | max rel diff. = 3567.49
40
+ [2020-11-04 13:04:04.655] [jointLog] [info] iteration = 100 | max rel diff. = 12.72
41
+ [2020-11-04 13:04:07.641] [jointLog] [info] iteration = 200 | max rel diff. = 17.7229
42
+ [2020-11-04 13:04:10.717] [jointLog] [info] iteration = 300 | max rel diff. = 1.88919
43
+ [2020-11-04 13:04:13.681] [jointLog] [info] iteration = 400 | max rel diff. = 0.2253
44
+ [2020-11-04 13:04:16.643] [jointLog] [info] iteration = 500 | max rel diff. = 1.66062
45
+ [2020-11-04 13:04:19.617] [jointLog] [info] iteration = 600 | max rel diff. = 9.50988
46
+ [2020-11-04 13:04:22.565] [jointLog] [info] iteration = 700 | max rel diff. = 5.16145
47
+ [2020-11-04 13:04:25.545] [jointLog] [info] iteration = 800 | max rel diff. = 0.0809726
48
+ [2020-11-04 13:04:28.532] [jointLog] [info] iteration = 900 | max rel diff. = 0.0450134
49
+ [2020-11-04 13:04:31.485] [jointLog] [info] iteration = 1,000 | max rel diff. = 0.0606601
50
+ [2020-11-04 13:04:34.368] [jointLog] [info] iteration = 1,100 | max rel diff. = 0.0375022
51
+ [2020-11-04 13:04:37.336] [jointLog] [info] iteration = 1,200 | max rel diff. = 0.776045
52
+ [2020-11-04 13:04:40.303] [jointLog] [info] iteration = 1,300 | max rel diff. = 0.266075
53
+ [2020-11-04 13:04:43.317] [jointLog] [info] iteration = 1,400 | max rel diff. = 0.346001
54
+ [2020-11-04 13:04:46.394] [jointLog] [info] iteration = 1,500 | max rel diff. = 0.20014
55
+ [2020-11-04 13:04:49.456] [jointLog] [info] iteration = 1,600 | max rel diff. = 0.149391
56
+ [2020-11-04 13:04:52.512] [jointLog] [info] iteration = 1,700 | max rel diff. = 0.0392946
57
+ [2020-11-04 13:04:55.579] [jointLog] [info] iteration = 1,800 | max rel diff. = 0.0749323
58
+ [2020-11-04 13:04:56.006] [jointLog] [info] iteration = 1,815 | max rel diff. = 0.00642309
59
+ [2020-11-04 13:04:56.028] [jointLog] [info] Finished optimizer
60
+ [2020-11-04 13:04:56.028] [jointLog] [info] writing output
61
+
RNA-seq/10-HS707A_S10_salmon/aux_info/ambig_info.tsv ADDED
The diff for this file is too large to render. See raw diff
 
RNA-seq/10-HS707A_S10_salmon/aux_info/meta_info.json ADDED
@@ -0,0 +1,44 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ {
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+ "salmon_version": "0.14.1",
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+ "samp_type": "none",
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5
+ "quant_errors": [],
6
+ "num_libraries": 1,
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+ "library_types": [
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+ "ISR"
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+ ],
10
+ "frag_dist_length": 1001,
11
+ "seq_bias_correct": false,
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+ "gc_bias_correct": false,
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+ "num_bias_bins": 4096,
14
+ "mapping_type": "mapping",
15
+ "num_valid_targets": 228754,
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+ "num_decoy_targets": 0,
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+ "num_eq_classes": 431642,
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+ "serialized_eq_classes": false,
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+ "eq_class_properties": [
20
+ "range_factorized"
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+ ],
22
+ "length_classes": [
23
+ 539,
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+ 723,
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+ 1214,
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+ 2392,
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+ 104301
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+ ],
29
+ "index_seq_hash": "a88dfd39d4111a3be9390c28572775449c52b57824f4c9112b5685d24aee4edd",
30
+ "index_name_hash": "22abc86b365c289a4c33d2e2d16975f7631a75723823f5fee000f4d9d79d8415",
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+ "index_seq_hash512": "6e2df13a76443d061f6d821afa1b0f848dac0ffb032d6be1a23a7826b758dd4672a580a656a10a023690e7b1df889c68e92051f91bcf9d9d81a66fe4d454386e",
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+ "index_name_hash512": "f9b740308e77d6fc1f6b95fba3a4a3e12b59c77190a2fbaba46b6fd343cec404fbddff630e538aa326a75c8d711ac5e7952bb9c94030ebbe6ac96b45ee86f078",
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+ "num_bootstraps": 0,
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+ "num_processed": 9522549,
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+ "num_mapped": 6514484,
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+ "num_decoy_fragments": 0,
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+ "num_dovetail_fragments": 42813,
38
+ "num_fragments_filtered_vm": 150183,
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+ "num_alignments_below_threshold_for_mapped_fragments_vm": 911361,
40
+ "percent_mapped": 68.41113655597887,
41
+ "call": "quant",
42
+ "start_time": "Wed Nov 4 12:37:11 2020",
43
+ "end_time": "Wed Nov 4 12:37:58 2020"
44
+ }
RNA-seq/10-HS707A_S10_salmon/libParams/flenDist.txt ADDED
@@ -0,0 +1,2 @@
 
 
 
1
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2
+
RNA-seq/10-HS707A_S10_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,53 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 12:37:11.538] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 12:37:11.538] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 12:37:11.538] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 12:37:11.538] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 12:37:11.538] [jointLog] [info] parsing read library format
6
+ [2020-11-04 12:37:11.538] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 12:37:11.626] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 12:37:11.627] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 12:37:19.362] [jointLog] [info] done
10
+ [2020-11-04 12:37:19.362] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 12:37:41.677] [jointLog] [info] Thread saw mini-batch with a maximum of 3.04% zero probability fragments
12
+ [2020-11-04 12:37:41.680] [jointLog] [info] Thread saw mini-batch with a maximum of 2.78% zero probability fragments
13
+ [2020-11-04 12:37:41.699] [jointLog] [info] Thread saw mini-batch with a maximum of 2.96% zero probability fragments
14
+ [2020-11-04 12:37:41.718] [jointLog] [info] Thread saw mini-batch with a maximum of 2.76% zero probability fragments
15
+ [2020-11-04 12:37:41.720] [jointLog] [info] Thread saw mini-batch with a maximum of 2.98% zero probability fragments
16
+ [2020-11-04 12:37:41.720] [jointLog] [info] Thread saw mini-batch with a maximum of 2.76% zero probability fragments
17
+ [2020-11-04 12:37:41.727] [jointLog] [info] Thread saw mini-batch with a maximum of 3.10% zero probability fragments
18
+ [2020-11-04 12:37:41.728] [jointLog] [info] Thread saw mini-batch with a maximum of 2.80% zero probability fragments
19
+ [2020-11-04 12:37:41.761] [jointLog] [info] Thread saw mini-batch with a maximum of 2.90% zero probability fragments
20
+ [2020-11-04 12:37:41.769] [jointLog] [info] Thread saw mini-batch with a maximum of 2.72% zero probability fragments
21
+ [2020-11-04 12:37:41.770] [jointLog] [info] Thread saw mini-batch with a maximum of 3.04% zero probability fragments
22
+ [2020-11-04 12:37:41.782] [jointLog] [info] Thread saw mini-batch with a maximum of 3.10% zero probability fragments
23
+ [2020-11-04 12:37:42.105] [jointLog] [info] Computed 431,642 rich equivalence classes for further processing
24
+ [2020-11-04 12:37:42.105] [jointLog] [info] Counted 6,514,484 total reads in the equivalence classes
25
+ [2020-11-04 12:37:42.124] [jointLog] [info] Number of mappings discarded because of alignment score : 911,361
26
+ [2020-11-04 12:37:42.124] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 150,183
27
+ [2020-11-04 12:37:42.124] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
28
+ [2020-11-04 12:37:42.124] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 42,813
29
+ [2020-11-04 12:37:42.124] [jointLog] [info] Mapping rate = 68.4111%
30
+
31
+ [2020-11-04 12:37:42.124] [jointLog] [info] finished quantifyLibrary()
32
+ [2020-11-04 12:37:42.126] [jointLog] [info] Starting optimizer
33
+ [2020-11-04 12:37:42.106] [fileLog] [info]
34
+ At end of round 0
35
+ ==================
36
+ Observed 9522549 total fragments (9522549 in most recent round)
37
+
38
+ [2020-11-04 12:37:42.290] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 12:37:42.306] [jointLog] [info] iteration = 0 | max rel diff. = 2469.37
40
+ [2020-11-04 12:37:43.738] [jointLog] [info] iteration = 100 | max rel diff. = 18.209
41
+ [2020-11-04 12:37:45.304] [jointLog] [info] iteration = 200 | max rel diff. = 16.1287
42
+ [2020-11-04 12:37:46.853] [jointLog] [info] iteration = 300 | max rel diff. = 0.624372
43
+ [2020-11-04 12:37:48.350] [jointLog] [info] iteration = 400 | max rel diff. = 0.125045
44
+ [2020-11-04 12:37:49.902] [jointLog] [info] iteration = 500 | max rel diff. = 0.195984
45
+ [2020-11-04 12:37:51.415] [jointLog] [info] iteration = 600 | max rel diff. = 0.221783
46
+ [2020-11-04 12:37:52.983] [jointLog] [info] iteration = 700 | max rel diff. = 3.46601
47
+ [2020-11-04 12:37:54.501] [jointLog] [info] iteration = 800 | max rel diff. = 0.144948
48
+ [2020-11-04 12:37:56.022] [jointLog] [info] iteration = 900 | max rel diff. = 0.0269093
49
+ [2020-11-04 12:37:57.537] [jointLog] [info] iteration = 1,000 | max rel diff. = 0.0618778
50
+ [2020-11-04 12:37:57.788] [jointLog] [info] iteration = 1,018 | max rel diff. = 0.00618339
51
+ [2020-11-04 12:37:57.806] [jointLog] [info] Finished optimizer
52
+ [2020-11-04 12:37:57.806] [jointLog] [info] writing output
53
+
RNA-seq/11-MCF7_S11_L001_R1_001.fastq.gz ADDED
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RNA-seq/11-MCF7_S11_salmon/aux_info/ambig_info.tsv ADDED
The diff for this file is too large to render. See raw diff
 
RNA-seq/11-MCF7_S11_salmon/aux_info/meta_info.json ADDED
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+ {
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+ "num_alignments_below_threshold_for_mapped_fragments_vm": 7473246,
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+ "percent_mapped": 51.645128136736399,
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+ "call": "quant",
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+ "start_time": "Wed Nov 4 12:38:00 2020",
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+ "end_time": "Wed Nov 4 12:40:36 2020"
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+ }
RNA-seq/11-MCF7_S11_salmon/cmd_info.json ADDED
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+ "salmon_version": "0.14.1",
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+ "validateMappings": [],
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+ "index": "gencode.v35.transcripts.salmon.index",
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+ "libType": "ISR",
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+ ],
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+ "mates2": [
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+ "11-MCF7_S11_L002_R2_001.fastq.gz"
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+ ],
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+ "threads": "12",
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+ "output": "11-MCF7_S11_salmon",
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+ "auxDir": "aux_info"
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+
RNA-seq/11-MCF7_S11_salmon/lib_format_counts.json ADDED
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1
+ {
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+ "read_files": "[ [ 11-MCF7_S11_L001_R1_001.fastq.gz, 11-MCF7_S11_L001_R2_001.fastq.gz], [ 11-MCF7_S11_L002_R1_001.fastq.gz, 11-MCF7_S11_L002_R2_001.fastq.gz] ]",
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+ "expected_format": "ISR",
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+ "compatible_fragment_ratio": 1.0,
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+ "num_compatible_fragments": 19377956,
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+ "num_assigned_fragments": 19377956,
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+ "num_frags_with_consistent_mappings": 16117687,
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+ "num_frags_with_inconsistent_or_orphan_mappings": 3260269,
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+ "MSF": 0,
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+ "ISR": 16117687,
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+ "SR": 1977187,
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+ }
RNA-seq/11-MCF7_S11_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,56 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 12:38:00.187] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 12:38:00.187] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 12:38:00.187] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 12:38:00.187] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 12:38:00.187] [jointLog] [info] parsing read library format
6
+ [2020-11-04 12:38:00.187] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 12:38:00.268] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 12:38:00.270] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 12:38:08.080] [jointLog] [info] done
10
+ [2020-11-04 12:38:08.080] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 12:39:36.271] [jointLog] [info] Thread saw mini-batch with a maximum of 3.70% zero probability fragments
12
+ [2020-11-04 12:39:36.273] [jointLog] [info] Thread saw mini-batch with a maximum of 3.98% zero probability fragments
13
+ [2020-11-04 12:39:36.284] [jointLog] [info] Thread saw mini-batch with a maximum of 3.92% zero probability fragments
14
+ [2020-11-04 12:39:36.286] [jointLog] [info] Thread saw mini-batch with a maximum of 3.82% zero probability fragments
15
+ [2020-11-04 12:39:36.300] [jointLog] [info] Thread saw mini-batch with a maximum of 3.78% zero probability fragments
16
+ [2020-11-04 12:39:36.319] [jointLog] [info] Thread saw mini-batch with a maximum of 4.06% zero probability fragments
17
+ [2020-11-04 12:39:36.329] [jointLog] [info] Thread saw mini-batch with a maximum of 4.02% zero probability fragments
18
+ [2020-11-04 12:39:36.350] [jointLog] [info] Thread saw mini-batch with a maximum of 3.82% zero probability fragments
19
+ [2020-11-04 12:39:36.381] [jointLog] [info] Thread saw mini-batch with a maximum of 3.82% zero probability fragments
20
+ [2020-11-04 12:39:36.385] [jointLog] [info] Thread saw mini-batch with a maximum of 4.00% zero probability fragments
21
+ [2020-11-04 12:39:36.386] [jointLog] [info] Thread saw mini-batch with a maximum of 3.82% zero probability fragments
22
+ [2020-11-04 12:39:36.402] [jointLog] [info] Thread saw mini-batch with a maximum of 3.92% zero probability fragments
23
+ [2020-11-04 12:39:37.187] [fileLog] [info]
24
+ At end of round 0
25
+ ==================
26
+ Observed 37521363 total fragments (37521363 in most recent round)
27
+
28
+ [2020-11-04 12:39:37.186] [jointLog] [info] Computed 1,035,044 rich equivalence classes for further processing
29
+ [2020-11-04 12:39:37.186] [jointLog] [info] Counted 19,377,956 total reads in the equivalence classes
30
+ [2020-11-04 12:39:37.204] [jointLog] [info] Number of mappings discarded because of alignment score : 7,473,246
31
+ [2020-11-04 12:39:37.204] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 839,900
32
+ [2020-11-04 12:39:37.204] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
33
+ [2020-11-04 12:39:37.204] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 128,395
34
+ [2020-11-04 12:39:37.204] [jointLog] [info] Mapping rate = 51.6451%
35
+
36
+ [2020-11-04 12:39:37.204] [jointLog] [info] finished quantifyLibrary()
37
+ [2020-11-04 12:39:37.207] [jointLog] [info] Starting optimizer
38
+ [2020-11-04 12:39:37.485] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 12:39:37.522] [jointLog] [info] iteration = 0 | max rel diff. = 5169.86
40
+ [2020-11-04 12:39:41.675] [jointLog] [info] iteration = 100 | max rel diff. = 13.9412
41
+ [2020-11-04 12:39:46.003] [jointLog] [info] iteration = 200 | max rel diff. = 17.6501
42
+ [2020-11-04 12:39:50.328] [jointLog] [info] iteration = 300 | max rel diff. = 4.0478
43
+ [2020-11-04 12:39:54.618] [jointLog] [info] iteration = 400 | max rel diff. = 0.998576
44
+ [2020-11-04 12:39:58.897] [jointLog] [info] iteration = 500 | max rel diff. = 0.121091
45
+ [2020-11-04 12:40:02.855] [jointLog] [info] iteration = 600 | max rel diff. = 19.9374
46
+ [2020-11-04 12:40:06.997] [jointLog] [info] iteration = 700 | max rel diff. = 0.122184
47
+ [2020-11-04 12:40:11.213] [jointLog] [info] iteration = 800 | max rel diff. = 0.0751677
48
+ [2020-11-04 12:40:15.356] [jointLog] [info] iteration = 900 | max rel diff. = 0.103884
49
+ [2020-11-04 12:40:19.519] [jointLog] [info] iteration = 1,000 | max rel diff. = 0.0995749
50
+ [2020-11-04 12:40:23.696] [jointLog] [info] iteration = 1,100 | max rel diff. = 0.122126
51
+ [2020-11-04 12:40:27.964] [jointLog] [info] iteration = 1,200 | max rel diff. = 0.060178
52
+ [2020-11-04 12:40:32.215] [jointLog] [info] iteration = 1,300 | max rel diff. = 0.200545
53
+ [2020-11-04 12:40:35.969] [jointLog] [info] iteration = 1,390 | max rel diff. = 0.00245728
54
+ [2020-11-04 12:40:35.986] [jointLog] [info] Finished optimizer
55
+ [2020-11-04 12:40:35.986] [jointLog] [info] writing output
56
+
RNA-seq/12-ZR-75-1_S12_salmon/aux_info/ambig_info.tsv ADDED
The diff for this file is too large to render. See raw diff
 
RNA-seq/12-ZR-75-1_S12_salmon/aux_info/meta_info.json ADDED
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1
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+ "num_libraries": 1,
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+ "library_types": [
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+ "ISR"
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+ "frag_dist_length": 1001,
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+ "seq_bias_correct": false,
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+ "num_valid_targets": 228754,
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+ "num_decoy_targets": 0,
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+ "num_eq_classes": 1005989,
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+ "length_classes": [
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+ "index_seq_hash": "a88dfd39d4111a3be9390c28572775449c52b57824f4c9112b5685d24aee4edd",
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+ "index_name_hash": "22abc86b365c289a4c33d2e2d16975f7631a75723823f5fee000f4d9d79d8415",
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+ "index_seq_hash512": "6e2df13a76443d061f6d821afa1b0f848dac0ffb032d6be1a23a7826b758dd4672a580a656a10a023690e7b1df889c68e92051f91bcf9d9d81a66fe4d454386e",
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+ "index_name_hash512": "f9b740308e77d6fc1f6b95fba3a4a3e12b59c77190a2fbaba46b6fd343cec404fbddff630e538aa326a75c8d711ac5e7952bb9c94030ebbe6ac96b45ee86f078",
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+ "num_bootstraps": 0,
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+ "num_processed": 36381233,
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+ "num_mapped": 18407383,
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+ "num_decoy_fragments": 0,
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+ "num_dovetail_fragments": 113693,
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+ "num_fragments_filtered_vm": 839403,
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+ "num_alignments_below_threshold_for_mapped_fragments_vm": 7520534,
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+ "percent_mapped": 50.59581955344944,
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+ "call": "quant",
42
+ "start_time": "Wed Nov 4 12:40:38 2020",
43
+ "end_time": "Wed Nov 4 12:43:20 2020"
44
+ }
RNA-seq/12-ZR-75-1_S12_salmon/cmd_info.json ADDED
@@ -0,0 +1,17 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ {
2
+ "salmon_version": "0.14.1",
3
+ "validateMappings": [],
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+ "index": "gencode.v35.transcripts.salmon.index",
5
+ "libType": "ISR",
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+ "mates1": [
7
+ "12-ZR-75-1_S12_L001_R1_001.fastq.gz",
8
+ "12-ZR-75-1_S12_L002_R1_001.fastq.gz"
9
+ ],
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+ "mates2": [
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+ "12-ZR-75-1_S12_L001_R2_001.fastq.gz",
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+ "12-ZR-75-1_S12_L002_R2_001.fastq.gz"
13
+ ],
14
+ "threads": "12",
15
+ "output": "12-ZR-75-1_S12_salmon",
16
+ "auxDir": "aux_info"
17
+ }
RNA-seq/12-ZR-75-1_S12_salmon/libParams/flenDist.txt ADDED
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+
RNA-seq/12-ZR-75-1_S12_salmon/lib_format_counts.json ADDED
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RNA-seq/12-ZR-75-1_S12_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,58 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 12:40:38.919] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 12:40:38.919] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 12:40:38.919] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 12:40:38.919] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 12:40:38.919] [jointLog] [info] parsing read library format
6
+ [2020-11-04 12:40:38.919] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 12:40:38.997] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 12:40:38.999] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 12:40:46.849] [jointLog] [info] done
10
+ [2020-11-04 12:40:46.849] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 12:42:12.304] [jointLog] [info] Thread saw mini-batch with a maximum of 4.04% zero probability fragments
12
+ [2020-11-04 12:42:12.356] [jointLog] [info] Thread saw mini-batch with a maximum of 3.80% zero probability fragments
13
+ [2020-11-04 12:42:12.364] [jointLog] [info] Thread saw mini-batch with a maximum of 3.96% zero probability fragments
14
+ [2020-11-04 12:42:12.369] [jointLog] [info] Thread saw mini-batch with a maximum of 4.38% zero probability fragments
15
+ [2020-11-04 12:42:12.376] [jointLog] [info] Thread saw mini-batch with a maximum of 3.84% zero probability fragments
16
+ [2020-11-04 12:42:12.385] [jointLog] [info] Thread saw mini-batch with a maximum of 3.96% zero probability fragments
17
+ [2020-11-04 12:42:12.391] [jointLog] [info] Thread saw mini-batch with a maximum of 4.16% zero probability fragments
18
+ [2020-11-04 12:42:12.395] [jointLog] [info] Thread saw mini-batch with a maximum of 4.02% zero probability fragments
19
+ [2020-11-04 12:42:12.399] [jointLog] [info] Thread saw mini-batch with a maximum of 3.90% zero probability fragments
20
+ [2020-11-04 12:42:12.414] [jointLog] [info] Thread saw mini-batch with a maximum of 4.02% zero probability fragments
21
+ [2020-11-04 12:42:12.420] [jointLog] [info] Thread saw mini-batch with a maximum of 3.86% zero probability fragments
22
+ [2020-11-04 12:42:12.434] [jointLog] [info] Thread saw mini-batch with a maximum of 3.90% zero probability fragments
23
+ [2020-11-04 12:42:13.352] [fileLog] [info]
24
+ At end of round 0
25
+ ==================
26
+ Observed 36381233 total fragments (36381233 in most recent round)
27
+
28
+ [2020-11-04 12:42:13.351] [jointLog] [info] Computed 1,005,989 rich equivalence classes for further processing
29
+ [2020-11-04 12:42:13.351] [jointLog] [info] Counted 18,407,383 total reads in the equivalence classes
30
+ [2020-11-04 12:42:13.371] [jointLog] [info] Number of mappings discarded because of alignment score : 7,520,534
31
+ [2020-11-04 12:42:13.371] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 839,403
32
+ [2020-11-04 12:42:13.372] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
33
+ [2020-11-04 12:42:13.372] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 113,693
34
+ [2020-11-04 12:42:13.372] [jointLog] [info] Mapping rate = 50.5958%
35
+
36
+ [2020-11-04 12:42:13.372] [jointLog] [info] finished quantifyLibrary()
37
+ [2020-11-04 12:42:13.374] [jointLog] [info] Starting optimizer
38
+ [2020-11-04 12:42:13.717] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 12:42:13.758] [jointLog] [info] iteration = 0 | max rel diff. = 5083.12
40
+ [2020-11-04 12:42:17.842] [jointLog] [info] iteration = 100 | max rel diff. = 16.6829
41
+ [2020-11-04 12:42:22.103] [jointLog] [info] iteration = 200 | max rel diff. = 7.34351
42
+ [2020-11-04 12:42:26.341] [jointLog] [info] iteration = 300 | max rel diff. = 6.1079
43
+ [2020-11-04 12:42:30.520] [jointLog] [info] iteration = 400 | max rel diff. = 0.363587
44
+ [2020-11-04 12:42:34.672] [jointLog] [info] iteration = 500 | max rel diff. = 0.227366
45
+ [2020-11-04 12:42:38.825] [jointLog] [info] iteration = 600 | max rel diff. = 0.213214
46
+ [2020-11-04 12:42:42.989] [jointLog] [info] iteration = 700 | max rel diff. = 0.0793234
47
+ [2020-11-04 12:42:47.125] [jointLog] [info] iteration = 800 | max rel diff. = 3.48617
48
+ [2020-11-04 12:42:51.276] [jointLog] [info] iteration = 900 | max rel diff. = 0.0486687
49
+ [2020-11-04 12:42:55.380] [jointLog] [info] iteration = 1,000 | max rel diff. = 0.14654
50
+ [2020-11-04 12:42:59.495] [jointLog] [info] iteration = 1,100 | max rel diff. = 0.142484
51
+ [2020-11-04 12:43:03.560] [jointLog] [info] iteration = 1,200 | max rel diff. = 0.100617
52
+ [2020-11-04 12:43:07.646] [jointLog] [info] iteration = 1,300 | max rel diff. = 0.0308427
53
+ [2020-11-04 12:43:11.730] [jointLog] [info] iteration = 1,400 | max rel diff. = 0.226073
54
+ [2020-11-04 12:43:15.830] [jointLog] [info] iteration = 1,500 | max rel diff. = 0.0595172
55
+ [2020-11-04 12:43:19.560] [jointLog] [info] iteration = 1,592 | max rel diff. = 0.00136092
56
+ [2020-11-04 12:43:19.578] [jointLog] [info] Finished optimizer
57
+ [2020-11-04 12:43:19.578] [jointLog] [info] writing output
58
+
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2
+
RNA-seq/14-BxPC3_S14_salmon/lib_format_counts.json ADDED
@@ -0,0 +1,21 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ {
2
+ "read_files": "[ [ 14-BxPC3_S14_L001_R1_001.fastq.gz, 14-BxPC3_S14_L001_R2_001.fastq.gz], [ 14-BxPC3_S14_L002_R1_001.fastq.gz, 14-BxPC3_S14_L002_R2_001.fastq.gz] ]",
3
+ "expected_format": "ISR",
4
+ "compatible_fragment_ratio": 1.0,
5
+ "num_compatible_fragments": 10538764,
6
+ "num_assigned_fragments": 10538764,
7
+ "num_frags_with_consistent_mappings": 8994474,
8
+ "num_frags_with_inconsistent_or_orphan_mappings": 1544290,
9
+ "MSF": 0,
10
+ "OSF": 0,
11
+ "ISF": 0,
12
+ "MSR": 0,
13
+ "OSR": 0,
14
+ "ISR": 8994474,
15
+ "SF": 517715,
16
+ "SR": 1026575,
17
+ "MU": 0,
18
+ "OU": 0,
19
+ "IU": 0,
20
+ "U": 0
21
+ }
RNA-seq/14-BxPC3_S14_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,52 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 12:44:11.288] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 12:44:11.288] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 12:44:11.288] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 12:44:11.288] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 12:44:11.288] [jointLog] [info] parsing read library format
6
+ [2020-11-04 12:44:11.288] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 12:44:11.364] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 12:44:11.366] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 12:44:19.096] [jointLog] [info] done
10
+ [2020-11-04 12:44:19.096] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 12:44:58.670] [jointLog] [info] Thread saw mini-batch with a maximum of 3.16% zero probability fragments
12
+ [2020-11-04 12:44:58.674] [jointLog] [info] Thread saw mini-batch with a maximum of 3.08% zero probability fragments
13
+ [2020-11-04 12:44:58.676] [jointLog] [info] Thread saw mini-batch with a maximum of 2.96% zero probability fragments
14
+ [2020-11-04 12:44:58.685] [jointLog] [info] Thread saw mini-batch with a maximum of 3.04% zero probability fragments
15
+ [2020-11-04 12:44:58.704] [jointLog] [info] Thread saw mini-batch with a maximum of 2.98% zero probability fragments
16
+ [2020-11-04 12:44:58.716] [jointLog] [info] Thread saw mini-batch with a maximum of 2.92% zero probability fragments
17
+ [2020-11-04 12:44:58.733] [jointLog] [info] Thread saw mini-batch with a maximum of 2.96% zero probability fragments
18
+ [2020-11-04 12:44:58.745] [jointLog] [info] Thread saw mini-batch with a maximum of 3.20% zero probability fragments
19
+ [2020-11-04 12:44:58.746] [jointLog] [info] Thread saw mini-batch with a maximum of 2.86% zero probability fragments
20
+ [2020-11-04 12:44:58.747] [jointLog] [info] Thread saw mini-batch with a maximum of 2.94% zero probability fragments
21
+ [2020-11-04 12:44:58.749] [jointLog] [info] Thread saw mini-batch with a maximum of 2.96% zero probability fragments
22
+ [2020-11-04 12:44:58.754] [jointLog] [info] Thread saw mini-batch with a maximum of 2.88% zero probability fragments
23
+ [2020-11-04 12:44:59.285] [jointLog] [info] Computed 614,606 rich equivalence classes for further processing
24
+ [2020-11-04 12:44:59.285] [jointLog] [info] Counted 10,538,764 total reads in the equivalence classes
25
+ [2020-11-04 12:44:59.303] [jointLog] [info] Number of mappings discarded because of alignment score : 2,128,254
26
+ [2020-11-04 12:44:59.303] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 341,322
27
+ [2020-11-04 12:44:59.303] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
28
+ [2020-11-04 12:44:59.303] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 67,227
29
+ [2020-11-04 12:44:59.303] [jointLog] [info] Mapping rate = 59.6375%
30
+
31
+ [2020-11-04 12:44:59.303] [jointLog] [info] finished quantifyLibrary()
32
+ [2020-11-04 12:44:59.306] [jointLog] [info] Starting optimizer
33
+ [2020-11-04 12:44:59.286] [fileLog] [info]
34
+ At end of round 0
35
+ ==================
36
+ Observed 17671360 total fragments (17671360 in most recent round)
37
+
38
+ [2020-11-04 12:44:59.509] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 12:44:59.531] [jointLog] [info] iteration = 0 | max rel diff. = 3631.86
40
+ [2020-11-04 12:45:01.493] [jointLog] [info] iteration = 100 | max rel diff. = 14.927
41
+ [2020-11-04 12:45:03.655] [jointLog] [info] iteration = 200 | max rel diff. = 11.0617
42
+ [2020-11-04 12:45:05.901] [jointLog] [info] iteration = 300 | max rel diff. = 1.9949
43
+ [2020-11-04 12:45:07.979] [jointLog] [info] iteration = 400 | max rel diff. = 3.1444
44
+ [2020-11-04 12:45:10.146] [jointLog] [info] iteration = 500 | max rel diff. = 0.90122
45
+ [2020-11-04 12:45:12.189] [jointLog] [info] iteration = 600 | max rel diff. = 7.33963
46
+ [2020-11-04 12:45:14.263] [jointLog] [info] iteration = 700 | max rel diff. = 0.480069
47
+ [2020-11-04 12:45:16.330] [jointLog] [info] iteration = 800 | max rel diff. = 0.098915
48
+ [2020-11-04 12:45:18.394] [jointLog] [info] iteration = 900 | max rel diff. = 0.04559
49
+ [2020-11-04 12:45:20.542] [jointLog] [info] iteration = 999 | max rel diff. = 0.00828178
50
+ [2020-11-04 12:45:20.559] [jointLog] [info] Finished optimizer
51
+ [2020-11-04 12:45:20.559] [jointLog] [info] writing output
52
+
RNA-seq/15-Colo320_S15_salmon/aux_info/ambig_info.tsv ADDED
The diff for this file is too large to render. See raw diff
 
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+ "num_compatible_fragments": 29773109,
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+ "num_assigned_fragments": 29773109,
7
+ "num_frags_with_consistent_mappings": 23341678,
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+ "num_frags_with_inconsistent_or_orphan_mappings": 6431431,
9
+ "MSF": 0,
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RNA-seq/15-Colo320_S15_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,56 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 12:45:22.952] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 12:45:22.952] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 12:45:22.952] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 12:45:22.952] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 12:45:22.952] [jointLog] [info] parsing read library format
6
+ [2020-11-04 12:45:22.952] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 12:45:23.026] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 12:45:23.029] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 12:45:30.823] [jointLog] [info] done
10
+ [2020-11-04 12:45:30.823] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 12:48:13.871] [jointLog] [info] Thread saw mini-batch with a maximum of 4.32% zero probability fragments
12
+ [2020-11-04 12:48:13.874] [jointLog] [info] Thread saw mini-batch with a maximum of 4.40% zero probability fragments
13
+ [2020-11-04 12:48:13.900] [jointLog] [info] Thread saw mini-batch with a maximum of 4.42% zero probability fragments
14
+ [2020-11-04 12:48:13.905] [jointLog] [info] Thread saw mini-batch with a maximum of 4.30% zero probability fragments
15
+ [2020-11-04 12:48:13.908] [jointLog] [info] Thread saw mini-batch with a maximum of 4.50% zero probability fragments
16
+ [2020-11-04 12:48:13.912] [jointLog] [info] Thread saw mini-batch with a maximum of 4.38% zero probability fragments
17
+ [2020-11-04 12:48:13.923] [jointLog] [info] Thread saw mini-batch with a maximum of 4.50% zero probability fragments
18
+ [2020-11-04 12:48:13.936] [jointLog] [info] Thread saw mini-batch with a maximum of 4.36% zero probability fragments
19
+ [2020-11-04 12:48:13.964] [jointLog] [info] Thread saw mini-batch with a maximum of 4.56% zero probability fragments
20
+ [2020-11-04 12:48:13.971] [jointLog] [info] Thread saw mini-batch with a maximum of 4.26% zero probability fragments
21
+ [2020-11-04 12:48:13.971] [jointLog] [info] Thread saw mini-batch with a maximum of 4.22% zero probability fragments
22
+ [2020-11-04 12:48:13.975] [jointLog] [info] Thread saw mini-batch with a maximum of 4.34% zero probability fragments
23
+ [2020-11-04 12:48:15.332] [fileLog] [info]
24
+ At end of round 0
25
+ ==================
26
+ Observed 69619954 total fragments (69619954 in most recent round)
27
+
28
+ [2020-11-04 12:48:15.332] [jointLog] [info] Computed 1,488,961 rich equivalence classes for further processing
29
+ [2020-11-04 12:48:15.332] [jointLog] [info] Counted 29,773,109 total reads in the equivalence classes
30
+ [2020-11-04 12:48:15.348] [jointLog] [info] Number of mappings discarded because of alignment score : 17,528,289
31
+ [2020-11-04 12:48:15.348] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 1,774,391
32
+ [2020-11-04 12:48:15.348] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
33
+ [2020-11-04 12:48:15.348] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 211,511
34
+ [2020-11-04 12:48:15.348] [jointLog] [info] Mapping rate = 42.7652%
35
+
36
+ [2020-11-04 12:48:15.348] [jointLog] [info] finished quantifyLibrary()
37
+ [2020-11-04 12:48:15.350] [jointLog] [info] Starting optimizer
38
+ [2020-11-04 12:48:15.721] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 12:48:15.785] [jointLog] [info] iteration = 0 | max rel diff. = 5251.52
40
+ [2020-11-04 12:48:22.124] [jointLog] [info] iteration = 100 | max rel diff. = 12.5181
41
+ [2020-11-04 12:48:28.954] [jointLog] [info] iteration = 200 | max rel diff. = 8.12998
42
+ [2020-11-04 12:48:35.843] [jointLog] [info] iteration = 300 | max rel diff. = 2.14191
43
+ [2020-11-04 12:48:42.629] [jointLog] [info] iteration = 400 | max rel diff. = 5.43497
44
+ [2020-11-04 12:48:49.226] [jointLog] [info] iteration = 500 | max rel diff. = 2.6871
45
+ [2020-11-04 12:48:55.789] [jointLog] [info] iteration = 600 | max rel diff. = 0.0390864
46
+ [2020-11-04 12:49:02.440] [jointLog] [info] iteration = 700 | max rel diff. = 1.49226
47
+ [2020-11-04 12:49:08.917] [jointLog] [info] iteration = 800 | max rel diff. = 1.67571
48
+ [2020-11-04 12:49:15.573] [jointLog] [info] iteration = 900 | max rel diff. = 0.0642987
49
+ [2020-11-04 12:49:22.239] [jointLog] [info] iteration = 1,000 | max rel diff. = 0.283463
50
+ [2020-11-04 12:49:28.872] [jointLog] [info] iteration = 1,100 | max rel diff. = 0.312545
51
+ [2020-11-04 12:49:35.555] [jointLog] [info] iteration = 1,200 | max rel diff. = 0.160993
52
+ [2020-11-04 12:49:42.163] [jointLog] [info] iteration = 1,300 | max rel diff. = 0.892853
53
+ [2020-11-04 12:49:47.287] [jointLog] [info] iteration = 1,377 | max rel diff. = 0.00925329
54
+ [2020-11-04 12:49:47.304] [jointLog] [info] Finished optimizer
55
+ [2020-11-04 12:49:47.304] [jointLog] [info] writing output
56
+
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+ "call": "quant",
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+ "start_time": "Wed Nov 4 12:51:10 2020",
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+ "end_time": "Wed Nov 4 12:52:54 2020"
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+ }
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+ "threads": "12",
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+ "auxDir": "aux_info"
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2
+
RNA-seq/16-HCC44_S16_salmon/lib_format_counts.json ADDED
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1
+ {
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+ "read_files": "[ [ 16-HCC44_S16_L001_R1_001.fastq.gz, 16-HCC44_S16_L001_R2_001.fastq.gz], [ 16-HCC44_S16_L002_R1_001.fastq.gz, 16-HCC44_S16_L002_R2_001.fastq.gz] ]",
3
+ "expected_format": "ISR",
4
+ "compatible_fragment_ratio": 1.0,
5
+ "num_compatible_fragments": 17801313,
6
+ "num_assigned_fragments": 17801313,
7
+ "num_frags_with_consistent_mappings": 15224659,
8
+ "num_frags_with_inconsistent_or_orphan_mappings": 2576654,
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+ "MSF": 0,
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+ "OSF": 0,
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+ "ISF": 0,
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+ "MSR": 0,
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+ "OSR": 0,
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+ "ISR": 15224659,
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+ "SF": 924551,
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+ "SR": 1652103,
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+ "MU": 0,
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+ "OU": 0,
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+ "IU": 0,
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+ "U": 0
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+ }
RNA-seq/16-HCC44_S16_salmon/logs/salmon_quant.log ADDED
@@ -0,0 +1,54 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ [2020-11-04 12:51:10.603] [jointLog] [info] Fragment incompatibility prior below threshold. Incompatible fragments will be ignored.
2
+ [2020-11-04 12:51:10.603] [jointLog] [info] Usage of --validateMappings implies use of minScoreFraction. Since not explicitly specified, it is being set to 0.65
3
+ [2020-11-04 12:51:10.603] [jointLog] [info] Usage of --validateMappings, without --hardFilter implies use of range factorization. rangeFactorizationBins is being set to 4
4
+ [2020-11-04 12:51:10.603] [jointLog] [info] Usage of --validateMappings implies a default consensus slack of 0.2. Setting consensusSlack to 0.2.
5
+ [2020-11-04 12:51:10.603] [jointLog] [info] parsing read library format
6
+ [2020-11-04 12:51:10.603] [jointLog] [info] There is 1 library.
7
+ [2020-11-04 12:51:10.693] [jointLog] [info] Loading Quasi index
8
+ [2020-11-04 12:51:10.695] [jointLog] [info] Loading 32-bit quasi index
9
+ [2020-11-04 12:51:18.439] [jointLog] [info] done
10
+ [2020-11-04 12:51:18.439] [jointLog] [info] Index contained 228,754 targets
11
+ [2020-11-04 12:52:23.169] [jointLog] [info] Thread saw mini-batch with a maximum of 3.08% zero probability fragments
12
+ [2020-11-04 12:52:23.178] [jointLog] [info] Thread saw mini-batch with a maximum of 3.26% zero probability fragments
13
+ [2020-11-04 12:52:23.185] [jointLog] [info] Thread saw mini-batch with a maximum of 3.36% zero probability fragments
14
+ [2020-11-04 12:52:23.200] [jointLog] [info] Thread saw mini-batch with a maximum of 3.44% zero probability fragments
15
+ [2020-11-04 12:52:23.216] [jointLog] [info] Thread saw mini-batch with a maximum of 3.40% zero probability fragments
16
+ [2020-11-04 12:52:23.219] [jointLog] [info] Thread saw mini-batch with a maximum of 3.22% zero probability fragments
17
+ [2020-11-04 12:52:23.226] [jointLog] [info] Thread saw mini-batch with a maximum of 3.10% zero probability fragments
18
+ [2020-11-04 12:52:23.237] [jointLog] [info] Thread saw mini-batch with a maximum of 3.04% zero probability fragments
19
+ [2020-11-04 12:52:23.251] [jointLog] [info] Thread saw mini-batch with a maximum of 3.04% zero probability fragments
20
+ [2020-11-04 12:52:23.259] [jointLog] [info] Thread saw mini-batch with a maximum of 3.12% zero probability fragments
21
+ [2020-11-04 12:52:23.274] [jointLog] [info] Thread saw mini-batch with a maximum of 3.20% zero probability fragments
22
+ [2020-11-04 12:52:23.275] [jointLog] [info] Thread saw mini-batch with a maximum of 3.10% zero probability fragments
23
+ [2020-11-04 12:52:23.891] [fileLog] [info]
24
+ At end of round 0
25
+ ==================
26
+ Observed 29173177 total fragments (29173177 in most recent round)
27
+
28
+ [2020-11-04 12:52:23.891] [jointLog] [info] Computed 761,059 rich equivalence classes for further processing
29
+ [2020-11-04 12:52:23.891] [jointLog] [info] Counted 17,801,313 total reads in the equivalence classes
30
+ [2020-11-04 12:52:23.910] [jointLog] [info] Number of mappings discarded because of alignment score : 3,240,832
31
+ [2020-11-04 12:52:23.910] [jointLog] [info] Number of fragments entirely discarded because of alignment score : 500,810
32
+ [2020-11-04 12:52:23.910] [jointLog] [info] Number of fragments discarded because they are best-mapped to decoys : 0
33
+ [2020-11-04 12:52:23.910] [jointLog] [info] Number of fragments discarded because they have only dovetail (discordant) mappings : 118,182
34
+ [2020-11-04 12:52:23.910] [jointLog] [info] Mapping rate = 61.0195%
35
+
36
+ [2020-11-04 12:52:23.910] [jointLog] [info] finished quantifyLibrary()
37
+ [2020-11-04 12:52:23.913] [jointLog] [info] Starting optimizer
38
+ [2020-11-04 12:52:24.171] [jointLog] [info] Marked 0 weighted equivalence classes as degenerate
39
+ [2020-11-04 12:52:24.197] [jointLog] [info] iteration = 0 | max rel diff. = 4999.15
40
+ [2020-11-04 12:52:26.847] [jointLog] [info] iteration = 100 | max rel diff. = 11.1931
41
+ [2020-11-04 12:52:29.570] [jointLog] [info] iteration = 200 | max rel diff. = 14.4542
42
+ [2020-11-04 12:52:32.329] [jointLog] [info] iteration = 300 | max rel diff. = 3.24777
43
+ [2020-11-04 12:52:34.859] [jointLog] [info] iteration = 400 | max rel diff. = 2.56899
44
+ [2020-11-04 12:52:37.554] [jointLog] [info] iteration = 500 | max rel diff. = 6.04014
45
+ [2020-11-04 12:52:40.182] [jointLog] [info] iteration = 600 | max rel diff. = 0.376957
46
+ [2020-11-04 12:52:42.862] [jointLog] [info] iteration = 700 | max rel diff. = 0.190514
47
+ [2020-11-04 12:52:45.355] [jointLog] [info] iteration = 800 | max rel diff. = 0.0949489
48
+ [2020-11-04 12:52:47.931] [jointLog] [info] iteration = 900 | max rel diff. = 0.198818
49
+ [2020-11-04 12:52:50.486] [jointLog] [info] iteration = 1,000 | max rel diff. = 0.122703
50
+ [2020-11-04 12:52:53.222] [jointLog] [info] iteration = 1,100 | max rel diff. = 0.0890821
51
+ [2020-11-04 12:52:54.159] [jointLog] [info] iteration = 1,136 | max rel diff. = 0.00562987
52
+ [2020-11-04 12:52:54.181] [jointLog] [info] Finished optimizer
53
+ [2020-11-04 12:52:54.181] [jointLog] [info] writing output
54
+
RNA-seq/17-HEK293T-3_S17_salmon/aux_info/ambig_info.tsv ADDED
The diff for this file is too large to render. See raw diff
 
RNA-seq/17-HEK293T-3_S17_salmon/aux_info/meta_info.json ADDED
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1
+ {
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+ "salmon_version": "0.14.1",
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+ "samp_type": "none",
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+ "opt_type": "vb",
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+ "quant_errors": [],
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+ "num_libraries": 1,
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+ "library_types": [
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+ "ISR"
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+ ],
10
+ "frag_dist_length": 1001,
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+ "seq_bias_correct": false,
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+ "gc_bias_correct": false,
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+ "num_bias_bins": 4096,
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+ "mapping_type": "mapping",
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+ "num_valid_targets": 228754,
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+ "num_decoy_targets": 0,
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+ "num_eq_classes": 1160354,
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+ "serialized_eq_classes": false,
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+ "eq_class_properties": [
20
+ "range_factorized"
21
+ ],
22
+ "length_classes": [
23
+ 539,
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+ 723,
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+ 1214,
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+ 2392,
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+ 104301
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+ ],
29
+ "index_seq_hash": "a88dfd39d4111a3be9390c28572775449c52b57824f4c9112b5685d24aee4edd",
30
+ "index_name_hash": "22abc86b365c289a4c33d2e2d16975f7631a75723823f5fee000f4d9d79d8415",
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+ "index_seq_hash512": "6e2df13a76443d061f6d821afa1b0f848dac0ffb032d6be1a23a7826b758dd4672a580a656a10a023690e7b1df889c68e92051f91bcf9d9d81a66fe4d454386e",
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+ "index_name_hash512": "f9b740308e77d6fc1f6b95fba3a4a3e12b59c77190a2fbaba46b6fd343cec404fbddff630e538aa326a75c8d711ac5e7952bb9c94030ebbe6ac96b45ee86f078",
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+ "num_bootstraps": 0,
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+ "num_processed": 42760454,
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+ "num_mapped": 21296839,
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+ "num_decoy_fragments": 0,
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+ "num_dovetail_fragments": 154976,
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+ "num_fragments_filtered_vm": 1005796,
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+ "num_alignments_below_threshold_for_mapped_fragments_vm": 9253141,
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+ "percent_mapped": 49.80498803871446,
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+ "call": "quant",
42
+ "start_time": "Wed Nov 4 12:52:56 2020",
43
+ "end_time": "Wed Nov 4 12:55:55 2020"
44
+ }
RNA-seq/17-HEK293T-3_S17_salmon/cmd_info.json ADDED
@@ -0,0 +1,17 @@
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
+ {
2
+ "salmon_version": "0.14.1",
3
+ "validateMappings": [],
4
+ "index": "gencode.v35.transcripts.salmon.index",
5
+ "libType": "ISR",
6
+ "mates1": [
7
+ "17-HEK293T-3_S17_L001_R1_001.fastq.gz",
8
+ "17-HEK293T-3_S17_L002_R1_001.fastq.gz"
9
+ ],
10
+ "mates2": [
11
+ "17-HEK293T-3_S17_L001_R2_001.fastq.gz",
12
+ "17-HEK293T-3_S17_L002_R2_001.fastq.gz"
13
+ ],
14
+ "threads": "12",
15
+ "output": "17-HEK293T-3_S17_salmon",
16
+ "auxDir": "aux_info"
17
+ }