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a_chr, a_min, a_max = a b_chr, b_min, b_max = b a_min, a_max = sorted((a_min, a_max)) b_min, b_max = sorted((b_min, b_max)) shorter = min((a_max - a_min), (b_max - b_min)) + 1 # must be on the same chromosome if a_chr != b_chr: ov = 0 else: ov = min(shorter, (a_max -...
def range_overlap(a, b, ratio=False)
Returns whether two ranges overlap. Set percentage=True returns overlap ratio over the shorter range of the two. >>> range_overlap(("1", 30, 45), ("1", 41, 55)) 5 >>> range_overlap(("1", 21, 45), ("1", 41, 75), ratio=True) 0.2 >>> range_overlap(("1", 30, 45), ("1", 15, 55)) 16 >>> range...
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assert distmode in ('ss', 'ee') a_chr, a_min, a_max, a_strand = a b_chr, b_min, b_max, b_strand = b # must be on the same chromosome if a_chr != b_chr: dist = -1 #elif range_overlap(a[:3], b[:3]): # dist = 0 else: # If the two ranges do not overlap, check strande...
def range_distance(a, b, distmode='ss')
Returns the distance between two ranges. distmode is ss, se, es, ee and sets the place on read one and two to measure the distance (s = start, e = end) >>> range_distance(("1", 30, 45, '+'), ("1", 45, 55, '+')) (26, '++') >>> range_distance(("1", 30, 45, '-'), ("1", 57, 68, '-')) (39, '-...
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rmin = min(ranges)[0] rmax = max(ranges, key=lambda x: x[1])[1] return rmin, rmax
def range_minmax(ranges)
Returns the span of a collection of ranges where start is the smallest of all starts, and end is the largest of all ends. >>> ranges = [(30, 45), (40, 50), (10, 100)] >>> range_minmax(ranges) (10, 100)
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from jcvi.utils.orderedcollections import SortedCollection key = (lambda x: x) if left else (lambda x: (x[0], x[2], x[1])) rr = SortedCollection(ranges, key=key) try: if left: s = rr.find_le(b) assert key(s) <= key(b), (s, b) else: s = rr.find_ge(...
def range_closest(ranges, b, left=True)
Returns the range that's closest to the given position. Notice that the behavior is to return ONE closest range to the left end (if left is True). This is a SLOW method. >>> ranges = [("1", 30, 40), ("1", 33, 35), ("1", 10, 20)] >>> b = ("1", 22, 25) >>> range_closest(ranges, b) ('1', 10, 20) ...
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from jcvi.utils.iter import pairwise ranges = range_merge(ranges) interleaved_ranges = [] for ch, cranges in groupby(ranges, key=lambda x: x[0]): cranges = list(cranges) size = sizes.get(ch, None) if size: ch, astart, aend = cranges[0] if astart > 1:...
def range_interleave(ranges, sizes={}, empty=False)
Returns the ranges in between the given ranges. >>> ranges = [("1", 30, 40), ("1", 45, 50), ("1", 10, 30)] >>> range_interleave(ranges) [('1', 41, 44)] >>> ranges = [("1", 30, 40), ("1", 42, 50)] >>> range_interleave(ranges) [('1', 41, 41)] >>> range_interleave(ranges, sizes={"1": 70}) ...
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if not ranges: return [] ranges.sort() cur_range = list(ranges[0]) merged_ranges = [] for r in ranges[1:]: # open new range if start > cur_end or seqid != cur_seqid if r[1] - cur_range[2] > dist or r[0] != cur_range[0]: merged_ranges.append(tuple(cur_range)...
def range_merge(ranges, dist=0)
Returns merged range. Similar to range_union, except this returns new ranges. >>> ranges = [("1", 30, 45), ("1", 40, 50), ("1", 10, 50)] >>> range_merge(ranges) [('1', 10, 50)] >>> ranges = [("1", 30, 40), ("1", 45, 50)] >>> range_merge(ranges) [('1', 30, 40), ('1', 45, 50)] >>> ranges ...
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if not ranges: return 0 ranges.sort() total_len = 0 cur_chr, cur_left, cur_right = ranges[0] # left-most range for r in ranges: # open new range if left > cur_right or chr != cur_chr if r[1] > cur_right or r[0] != cur_chr: total_len += cur_right - cur_left...
def range_union(ranges)
Returns total size of ranges, expect range as (chr, left, right) >>> ranges = [("1", 30, 45), ("1", 40, 50), ("1", 10, 50)] >>> range_union(ranges) 41 >>> ranges = [("1", 30, 45), ("2", 40, 50)] >>> range_union(ranges) 27 >>> ranges = [("1", 30, 45), ("1", 45, 50)] >>> range_union(range...
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if not ranges: return 0 ranges.sort() ans = 0 for seq, lt in groupby(ranges, key=lambda x: x[0]): lt = list(lt) ans += max(max(lt)[1:]) - min(min(lt)[1:]) + 1 return ans
def range_span(ranges)
Returns the total span between the left most range to the right most range. >>> ranges = [("1", 30, 45), ("1", 40, 50), ("1", 10, 50)] >>> range_span(ranges) 41 >>> ranges = [("1", 30, 45), ("2", 40, 50)] >>> range_span(ranges) 27 >>> ranges = [("1", 30, 45), ("1", 45, 50)] >>> range_sp...
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endpoints = _make_endpoints(ranges) for seqid, ends in groupby(endpoints, lambda x: x[0]): active = [] depth = 0 for seqid, pos, leftright, i, score in ends: if leftright == LEFT: active.append(i) depth += 1 else: ...
def range_piles(ranges)
Return piles of intervals that overlap. The piles are only interrupted by regions of zero coverage. >>> ranges = [Range("2", 0, 1, 3, 0), Range("2", 1, 4, 3, 1), Range("3", 5, 7, 3, 2)] >>> list(range_piles(ranges)) [[0, 1], [2]]
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overlap = set() active = set() endpoints = _make_endpoints(ranges) for seqid, ends in groupby(endpoints, lambda x: x[0]): active.clear() for seqid, pos, leftright, i, score in ends: if leftright == LEFT: active.add(i) else: ac...
def range_conflict(ranges, depth=1)
Find intervals that are overlapping in 1-dimension. Return groups of block IDs that are in conflict. >>> ranges = [Range("2", 0, 1, 3, 0), Range("2", 1, 4, 3, 1), Range("3", 5, 7, 3, 2)] >>> list(range_conflict(ranges)) [(0, 1)]
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endpoints = _make_endpoints(ranges) # stores the left end index for quick retrieval left_index = {} # dynamic programming, each entry [score, from_index, which_chain] scores = [] for i, (seqid, pos, leftright, j, score) in enumerate(endpoints): cur_score = [0, -1, -1] if i == 0 e...
def range_chain(ranges)
Take list of weighted intervals, find non-overlapping set with max weight. We proceed with each end point (sorted by their relative positions). The input are a list of ranges of the form (start, stop, score), output is subset of the non-overlapping ranges that give the highest score, score >>> ranges ...
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ranges.sort() for seqid, rrs in groupby(ranges, key=lambda x: x[0]): rrs = [(a, b) for (s, a, b) in rrs] size = sizes[seqid] ds, depthdetails = range_depth(rrs, size, verbose=verbose) depthdetails = [(seqid, s, e, d) for s, e, d in depthdetails] yield depthdetails
def ranges_depth(ranges, sizes, verbose=True)
Allow triple (seqid, start, end) rather than just tuple (start, end)
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from jcvi.utils.iter import pairwise from jcvi.utils.cbook import percentage # Make endpoints endpoints = [] for a, b in ranges: endpoints.append((a, LEFT)) endpoints.append((b, RIGHT)) endpoints.sort() vstart, vend = min(endpoints)[0], max(endpoints)[0] assert 0 <...
def range_depth(ranges, size, verbose=True)
Overlay ranges on [start, end], and summarize the ploidy of the intervals.
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formatted = load_csv(header, rows, sep=" ", thousands=thousands) header, rows = formatted[0], formatted[1:] return banner(header, rows)
def loadtable(header, rows, major='=', minor='-', thousands=True)
Print a tabular output, with horizontal separators
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pairs = d.keys() rows, cols = zip(*pairs) if transpose: rows, cols = cols, rows rows = sorted(set(rows)) cols = sorted(set(cols)) header = ["o"] + list(cols) table = [] for r in rows: combo = [(r, c) for c in cols] if transpose: combo = [(c, r) f...
def tabulate(d, transpose=False, thousands=True, key_fun=None, sep=',', align=True)
d is a dictionary, keyed by tuple(A, B). Goal is to put A in rows, B in columns, report data in table form. >>> d = {(1,'a'):3, (1,'b'):4, (2,'a'):5, (2,'b'):0} >>> print tabulate(d) =========== o a b ----------- 1 3 4 2 5 0 ----------- >>> print tabulate(d, tr...
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2.748505
1.00091
from jcvi.formats.base import must_open formatted = load_csv(header, contents, sep=sep, thousands=thousands, align=align) if comment: formatted[0] = '#' + formatted[0][1:] formatted = "\n".join(formatted) fw = must_open(filename, "w") print(formatted, file=...
def write_csv(header, contents, sep=",", filename="stdout", thousands=False, tee=False, align=True, comment=False)
Write csv that are aligned with the column headers. >>> header = ["x_value", "y_value"] >>> contents = [(1, 100), (2, 200)] >>> write_csv(header, contents) x_value, y_value 1, 100 2, 200
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4.132022
0.746063
from jcvi.formats.base import is_number from jcvi.formats.blast import best as blast_best, bed as blast_bed from jcvi.apps.align import blat as blat_align p = OptionParser(blat.__doc__) opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) maptxt, re...
def blat(args)
%prog blat map1.txt ref.fasta Make ALLMAPS input csv based on sequences. The tab-delimited txt file include: name, LG, position, sequence.
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2.729492
1.053341
from jcvi.assembly.allmaps import CSVMapLine from jcvi.formats.sizes import Sizes from jcvi.utils.natsort import natsorted from jcvi.graphics.base import shorten from jcvi.graphics.dotplot import plt, savefig, markup, normalize_axes, \ downsample, plot_breaks_and_labels, tho...
def dotplot(args)
%prog dotplot map.csv ref.fasta Make dotplot between chromosomes and linkage maps. The input map is csv formatted, for example: ScaffoldID,ScaffoldPosition,LinkageGroup,GeneticPosition scaffold_2707,11508,1,0 scaffold_2707,11525,1,1.2
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3.422986
0.998742
from jcvi.formats.base import DictFile p = OptionParser(header.__doc__) p.add_option("--prefix", default="", help="Prepend text to line number [default: %default]") p.add_option("--ids", help="Write ids to file [default: %default]") opts, args = p.parse_args(args) if len(...
def header(args)
%prog header map conversion_table Rename lines in the map header. The mapping of old names to new names are stored in two-column `conversion_table`.
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3.059796
1.087919
p = OptionParser(rename.__doc__) p.set_outfile() opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) mstmap, bedfile = args markersbed = Bed(bedfile) markers = markersbed.order data = MSTMap(mstmap) header = data.header header = [header...
def rename(args)
%prog rename map markers.bed > renamed.map Rename markers according to the new mapping locations.
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3.243754
1.088553
from jcvi.formats.blast import bed p = OptionParser(anchor.__doc__) opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) mapbed, blastfile = args bedfile = bed([blastfile]) markersbed = Bed(bedfile) markers = markersbed.order mapbed = Bed(m...
def anchor(args)
%prog anchor map.bed markers.blast > anchored.bed Anchor scaffolds based on map.
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3.295757
1.082294
p = OptionParser(bed.__doc__) p.add_option("--switch", default=False, action="store_true", help="Switch reference and aligned map elements [default: %default]") opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) mapout, = args pf = map...
def bed(args)
%prog fasta map.out Convert MSTMAP output into bed format.
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3.138759
1.119714
from jcvi.formats.sizes import Sizes p = OptionParser(fasta.__doc__) p.add_option("--extend", default=1000, type="int", help="Extend seq flanking the gaps [default: %default]") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) mapout...
def fasta(args)
%prog fasta map.out scaffolds.fasta Extract marker sequences based on map.
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3.27566
1.093686
from jcvi.utils.iter import pairwise p = OptionParser(breakpoint.__doc__) p.add_option("--diff", default=.1, type="float", help="Maximum ratio of differences allowed [default: %default]") opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) ...
def breakpoint(args)
%prog breakpoint mstmap.input > breakpoints.bed Find scaffold breakpoints using genetic map. Use variation.vcf.mstmap() to generate the input for this routine.
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2.984936
1.075216
start, end = line.component_beg, line.component_end size = end - start + 1 leftNs, rightNs = 0, 0 lid, lo = line.component_id, line.orientation for s in seq: if s in 'nN': leftNs += 1 else: break for s in seq[::-1]: if s in 'nN': r...
def trimNs(seq, line, newagp)
Test if the sequences contain dangling N's on both sides. This component needs to be adjusted to the 'actual' sequence range.
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2.741169
1.013436
import csv from jcvi.formats.sizes import Sizes p = OptionParser(fromcsv.__doc__) p.add_option("--evidence", default="map", help="Linkage evidence to add in AGP") opts, args = p.parse_args(args) if len(args) != 3: sys.exit(not p.print_help()) contigsfasta, ma...
def fromcsv(args)
%prog fromcsv contigs.fasta map.csv map.agp Convert csv which contains list of scaffolds/contigs to AGP file.
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1.205585
p = OptionParser(compress.__doc__) p.set_outfile() opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) aagpfile, bagpfile = args # First AGP provides the mapping store = {} agp = AGP(aagpfile) for a in agp: if a.is_gap: c...
def compress(args)
%prog compress a.agp b.agp Convert coordinates based on multiple AGP files. Useful to simplify multiple liftOvers to compress multiple chain files into a single chain file, in upgrading locations of genomic features. Example: `a.agp` could contain split scaffolds: scaffold_0.1 1 60030...
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2.910426
1.061964
from jcvi.apps.grid import WriteJobs from jcvi.formats.bed import sort p = OptionParser(infer.__doc__) p.set_cpus() opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) scaffoldsf, genomef = args inferbed = "infer-components.bed" if need_upd...
def infer(args)
%prog infer scaffolds.fasta genome.fasta Infer where the components are in the genome. This function is rarely used, but can be useful when distributor does not ship an AGP file.
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1.079489
from jcvi.formats.base import DictFile p = OptionParser(format.__doc__) p.add_option("--switchcomponent", help="Switch component id based on") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) oldagpfile, newagpfile = args switch...
def format(args)
%prog format oldagpfile newagpfile Reformat AGP file. --switch will replace the ids in the AGP file.
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2.462143
1.140995
p = OptionParser(frombed.__doc__) p.add_option("--gapsize", default=100, type="int", help="Insert gaps of size [default: %default]") opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) bedfile, = args gapsize = opts.gapsize agpfile ...
def frombed(args)
%prog frombed bedfile Generate AGP file based on bed file. The bed file must have at least 6 columns. With the 4-th column indicating the new object.
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2.905679
1.075474
from jcvi.utils.range import range_interleave p = OptionParser(swap.__doc__) opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) agpfile, = args agp = AGP(agpfile, nogaps=True, validate=False) agp.sort(key=lambda x: (x.component_id, x.component_be...
def swap(args)
%prog swap agpfile Swap objects and components. Will add gap lines. This is often used in conjuction with formats.chain.fromagp() to convert between different coordinate systems.
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from jcvi.utils.table import tabulate p = OptionParser(stats.__doc__) p.add_option("--warn", default=False, action="store_true", help="Warnings on small component spans [default: %default]") opts, args = p.parse_args(args) if len(args) != 1: sys.exit(p.print_help()) ...
def stats(args)
%prog stats agpfile Print out a report for length of gaps and components.
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1.092366
p = OptionParser(cut.__doc__) p.add_option("--sep", default=".", help="Separator for splits") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) agpfile, bedfile = args sep = opts.sep agp = AGP(agpfile) bed = Bed(bedfile) simple_agp = agp....
def cut(args)
%prog cut agpfile bedfile Cut at the boundaries of the ranges in the bedfile.
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1.043463
p = OptionParser(reindex.__doc__) p.add_option("--nogaps", default=False, action="store_true", help="Remove all gap lines [default: %default]") p.add_option("--inplace", default=False, action="store_true", help="Replace input file [default: %default]") opts, args =...
def reindex(args)
%prog agpfile assume the component line order is correct, modify coordinates, this is necessary mostly due to manual edits (insert/delete) that disrupts the target coordinates.
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2.511151
1.064939
from jcvi.utils.table import write_csv p = OptionParser(summary.__doc__) opts, args = p.parse_args(args) if len(args) != 1: sys.exit(p.print_help()) agpfile, = args header = "Chromosome #_Distinct #_Components #_Scaffolds " \ "Scaff_N50 Scaff_L50 Length".split() ...
def summary(args)
%prog summary agpfile print a table of scaffold statistics, number of BACs, no of scaffolds, scaffold N50, scaffold L50, actual sequence, PSMOL NNNs, PSMOL-length, % of PSMOL sequenced.
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s = rec.description chr = re.search(chr_pat, s) clone = re.search(clone_pat, s) chr = chr.group(1) if chr else "" clone = clone.group(1) if clone else "" return chr, clone
def get_clone(rec)
>>> get_clone("Medicago truncatula chromosome 2 clone mth2-48e18") ('2', 'mth2-48e18')
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1.153302
p = OptionParser(phase.__doc__) p.set_outfile() opts, args = p.parse_args(args) if len(args) < 1: sys.exit(not p.print_help()) fw = must_open(opts.outfile, "w") for gbfile in args: for rec in SeqIO.parse(gbfile, "gb"): bac_phase, keywords = get_phase(rec) ...
def phase(args)
%prog phase genbankfiles Input has to be gb file. Search the `KEYWORDS` section to look for PHASE. Also look for "chromosome" and "clone" in the definition line.
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1.25471
p = OptionParser(tpf.__doc__) p.add_option("--noversion", default=False, action="store_true", help="Remove trailing accession versions [default: %default]") p.add_option("--gaps", default=False, action="store_true", help="Include gaps in the output [default: %default]") opts...
def tpf(args)
%prog tpf agpfile Print out a list of ids, one per line. Also known as the Tiling Path. AC225490.9 chr6 Can optionally output scaffold gaps.
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2.820009
1.073403
from jcvi.formats.obo import validate_term p = OptionParser(bed.__doc__) p.add_option("--gaps", default=False, action="store_true", help="Only print bed lines for gaps [default: %default]") p.add_option("--nogaps", default=False, action="store_true", help="Do not print bed ...
def bed(args)
%prog bed agpfile print out the tiling paths in bed/gff3 format
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2.663605
1.030806
from jcvi.formats.sizes import Sizes p = OptionParser(extendbed.__doc__) p.add_option("--nogaps", default=False, action="store_true", help="Do not print bed lines for gaps [default: %default]") p.add_option("--bed12", default=False, action="store_true", help="Produce bed12 ...
def extendbed(args)
%prog extend agpfile componentfasta Extend the components to fill the component range. For example, a bed/gff3 file that was converted from the agp will contain only the BAC sequence intervals that are 'represented' - sometimes leaving the 5` and 3` out (those that overlap with adjacent sequences. This...
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2.862996
1.036477
from jcvi.graphics.histogram import loghistogram p = OptionParser(gaps.__doc__) p.add_option("--merge", dest="merge", default=False, action="store_true", help="Merge adjacent gaps (to conform to AGP specification)") p.add_option("--header", default=False, action="store_true", ...
def gaps(args)
%prog gaps agpfile Print out the distribution of gapsizes. Option --merge allows merging of adjacent gaps which is used by tidy().
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3.064405
1.045625
p = OptionParser(tidy.__doc__) p.add_option("--nogaps", default=False, action="store_true", help="Remove all gap lines [default: %default]") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(p.print_help()) agpfile, componentfasta = args originalagpfile ...
def tidy(args)
%prog tidy agpfile componentfasta Given an agp file, run through the following steps: 1. Trim components with dangling N's 2. Merge adjacent gaps 3. Trim gaps at the end of an object 4. Reindex the agp Final output is in `.tidy.agp`.
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2.382331
1.16198
p = OptionParser(build.__doc__) p.add_option("--newagp", dest="newagp", default=False, action="store_true", help="Check components to trim dangling N's [default: %default]") p.add_option("--novalidate", dest="novalidate", default=False, action="store_true", help="Don...
def build(args)
%prog build agpfile componentfasta targetfasta Build targetfasta based on info from agpfile
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2.346373
1.197304
p = OptionParser(validate.__doc__) opts, args = p.parse_args(args) try: agpfile, componentfasta, targetfasta = args except Exception as e: sys.exit(p.print_help()) agp = AGP(agpfile) build = Fasta(targetfasta) bacs = Fasta(componentfasta, index=False) # go throug...
def validate(args)
%prog validate agpfile componentfasta targetfasta validate consistency between agpfile and targetfasta
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3.058733
1.199148
d = {} for (i, x) in enumerate(self): if x.is_gap: continue xid = x.component_id d[xid] = (i, x) xid = xid.rsplit(".", 1)[0] # Remove Genbank version if xid not in d: d[xid] = (i, x) return d
def order(self)
Returns a dict with component_id => (i, agpline)
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1.345268
north = self.getAdjacentClone(i, south=False) south = self.getAdjacentClone(i) return north, south
def getNorthSouthClone(self, i)
Returns the adjacent clone name from both sides.
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3.992929
1.47477
print("\n".join(self.header), file=fw)
def transfer_header(self, fw=sys.stdout)
transfer_header() copies header to a new file. print_header() creates a new header.
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1.170012
components = [] total_bp = 0 for line in lines: if line.is_gap: seq = 'N' * line.gap_length if newagp: print(line, file=newagp) else: seq = fasta.sequence(dict(chr=line.component_id, ...
def build_one(self, object, lines, fasta, fw, newagp=None)
Construct molecule using component fasta sequence
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1.010769
rr = xrange(i + 1, len(self)) if south else xrange(i - 1, -1, -1) a = self[i] for ix in rr: x = self[ix] if x.object != a.object: break return x return None
def getAdjacentClone(self, i, south=True)
Returns adjacent clone name, either the line before or after the current line.
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from jcvi.formats.base import SetFile from jcvi.formats.gff import Gff p = OptionParser(pasa.__doc__) p.set_home("pasa") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) fastafile, gffile = args transcodergff = fastafile + ".transdecoder.gff...
def pasa(args)
%prog ${pasadb}.assemblies.fasta ${pasadb}.pasa_assemblies.gff3 Wraps `pasa_asmbls_to_training_set.dbi`.
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2.604823
1.12113
p = OptionParser(genemark.__doc__) p.add_option("--junctions", help="Path to `junctions.bed` from Tophat2") p.set_home("gmes") p.set_cpus(cpus=32) opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) species, fastafile = args junctions = opts.jun...
def genemark(args)
%prog genemark species fastafile Train GENEMARK model given fastafile. GENEMARK self-trains so no trainig model gff file is needed.
4.903391
4.63233
1.058515
p = OptionParser(snap.__doc__) p.set_home("maker") opts, args = p.parse_args(args) if len(args) != 3: sys.exit(not p.print_help()) species, gffile, fastafile = args mhome = opts.maker_home snapdir = "snap" mkdir(snapdir) cwd = os.getcwd() os.chdir(snapdir) ne...
def snap(args)
%prog snap species gffile fastafile Train SNAP model given gffile and fastafile. Whole procedure taken from: <http://gmod.org/wiki/MAKER_Tutorial_2012>
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4.106202
1.106339
p = OptionParser(augustus.__doc__) p.add_option("--autotrain", default=False, action="store_true", help="Run autoAugTrain.pl to iteratively train AUGUSTUS") p.set_home("augustus") opts, args = p.parse_args(args) if len(args) != 3: sys.exit(not p.print_help()) spec...
def augustus(args)
%prog augustus species gffile fastafile Train AUGUSTUS model given gffile and fastafile. Whole procedure taken from: <http://www.molecularevolution.org/molevolfiles/exercises/augustus/training.html>
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3.778232
1.060536
from collections import defaultdict p = OptionParser(prune.__doc__) add_graph_options(p) opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) bestedges, = args G = read_graph(bestedges, maxerr=opts.maxerr) reads_to_ctgs = parse_ctgs(bestedges, o...
def prune(args)
%prog prune best.edges Prune overlap graph.
2.989878
2.934553
1.018853
p = OptionParser(removecontains.__doc__) opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) contains, gkpStore = args s = set() fp = open(contains) for row in fp: if row[0] == '#': continue iid = int(row.split()[0]) ...
def removecontains(args)
%prog removecontains 4-unitigger/best.contains asm.gkpStore Remove contained reads from gkpStore. This will improve assembly contiguity without sacrificing accuracy, when using bogart unitigger.
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3.782345
1.139885
from jcvi.apps.console import green p = OptionParser(overlap.__doc__) p.add_option("--maxerr", default=2, type="int", help="Maximum error rate") p.add_option("--canvas", default=100, type="int", help="Canvas size") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p....
def overlap(args)
%prog overlap best.contains iid Visualize overlaps for a given fragment. Must be run in 4-unitigger. All overlaps for iid were retrieved, excluding the ones matching best.contains.
3.961695
3.751572
1.056009
p = OptionParser(merger.__doc__) opts, args = p.parse_args(args) if len(args) != 3: sys.exit(not p.print_help()) layout, gkpstore, contigs = args fp = open(layout) pf = "0" iidfile = pf + ".iids" for i, row in enumerate(fp): logging.debug("Read unitig {0}".format(i...
def merger(args)
%prog merger layout gkpStore contigs.fasta Merge reads into one contig.
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6.817116
1.113581
p = OptionParser(unitigs.__doc__) p.add_option("--maxerr", default=2, type="int", help="Maximum error rate") opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) bestedges, = args G = read_graph(bestedges, maxerr=opts.maxerr, directed=True) H = nx.Gr...
def unitigs(args)
%prog unitigs best.edges Reads Celera Assembler's "best.edges" and extract all unitigs.
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2.873889
1.062714
p = OptionParser(graph.__doc__) p.add_option("--query", default=-1, type="int", help="Search from node, -1 to select random node, 0 to disable") p.add_option("--contig", help="Search from contigs, use comma to separate") p.add_option("--largest", default=0, type="int", help="Only s...
def graph(args)
%prog graph best.edges Convert Celera Assembler's "best.edges" to a GEXF which can be used to feed into Gephi to check the topology of the best overlapping graph. Mutual best edges are represented as thicker edges. Reference: https://github.com/PacificBiosciences/Bioinformatics-Training/blob/maste...
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3.179338
1.038017
p = OptionParser(astat.__doc__) p.add_option("--cutoff", default=1000, type="int", help="Length cutoff [default: %default]") p.add_option("--genome", default="", help="Genome name [default: %default]") p.add_option("--arrDist", default=False, action="store_true", ...
def astat(args)
%prog astat coverage.log Create coverage-rho scatter plot.
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2.540494
1.021406
if fasta: s = SeqRecord(shredded_seq, id=fragID, description="") SeqIO.write([s], fw, "fasta") return seq = str(shredded_seq) slen = len(seq) qvs = qvchar * slen # shredded reads have default low qv if clr is None: clr_beg, clr_end = 0, slen else: ...
def emitFragment(fw, fragID, libID, shredded_seq, clr=None, qvchar='l', fasta=False)
Print out the shredded sequence.
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2.886835
1.013137
p = OptionParser(shred.__doc__) p.set_depth(depth=2) p.add_option("--readlen", default=1000, type="int", help="Desired length of the reads [default: %default]") p.add_option("--minctglen", default=0, type="int", help="Ignore contig sequence less than [default: %default]") ...
def shred(args)
%prog shred fastafile Similar to the method of `shredContig` in runCA script. The contigs are shredded into pseudo-reads with certain length and depth.
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2.694163
1.020334
p = OptionParser(tracedb.__doc__) opts, args = p.parse_args(args) if len(args) != 1: sys.exit(p.print_help()) action, = args assert action in ("xml", "lib", "frg") CMD = "tracedb-to-frg.pl" xmls = glob("xml*") if action == "xml": for xml in xmls: cmd...
def tracedb(args)
%prog tracedb <xml|lib|frg> Run `tracedb-to-frg.pl` within current folder.
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2.260542
1.315165
assert op.exists(fastafile) matefile = fastafile.rsplit(".", 1)[0] + ".mates" if op.exists(matefile): logging.debug("matepairs file `{0}` found".format(matefile)) else: logging.debug("parsing matepairs from `{0}`".format(fastafile)) matefw = open(matefile, "w") it =...
def make_matepairs(fastafile)
Assumes the mates are adjacent sequence records
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2.513575
0.984902
from jcvi.formats.fasta import clean, make_qual p = OptionParser(fasta.__doc__) p.add_option("--clean", default=False, action="store_true", help="Clean up irregular chars in seq") p.add_option("--matefile", help="Matepairs file") p.add_option("--maxreadlen", default=262143, ty...
def fasta(args)
%prog fasta fastafile Convert reads formatted as FASTA file, and convert to CA frg file. If .qual file is found, then use it, otherwise just make a fake qual file. Mates are assumed as adjacent sequence records (i.e. /1, /2, /1, /2 ...) unless a matefile is given.
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3.294873
1.026502
p = OptionParser(sff.__doc__) p.add_option("--prefix", dest="prefix", default=None, help="Output frg filename prefix") p.add_option("--nodedup", default=False, action="store_true", help="Do not remove duplicates [default: %default]") p.set_size() opts, args = p.parse_arg...
def sff(args)
%prog sff sffiles Convert reads formatted as 454 SFF file, and convert to CA frg file. Turn --nodedup on if another deduplication mechanism is used (e.g. CD-HIT-454). See assembly.sff.deduplicate().
4.257879
3.683872
1.155816
from jcvi.formats.fastq import guessoffset p = OptionParser(fastq.__doc__) p.add_option("--outtie", dest="outtie", default=False, action="store_true", help="Are these outie reads? [default: %default]") p.set_phred() p.set_size() opts, args = p.parse_args(args) if len(args...
def fastq(args)
%prog fastq fastqfile Convert reads formatted as FASTQ file, and convert to CA frg file.
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3.915254
1.031808
p = OptionParser(clr.__doc__) opts, args = p.parse_args(args) if len(args) < 2: sys.exit(not p.print_help()) blastfile = args[0] fastafiles = args[1:] sizes = {} for fa in fastafiles: f = Fasta(fa) sizes.update(f.itersizes()) b = Blast(blastfile) for ...
def clr(args)
%prog blastfile fastafiles Calculate the vector clear range file based BLAST to the vectors.
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2.366682
1.135969
import re if rule is None: return name k = re.search("(?<=^head)[0-9]{1,2}$", rule) if k: k = k.group(0) tname = name[int(k):] else: k = re.search("(?<=^ohead)[0-9]{1,2}$", rule) if k: k = k.group(0) tname = name[:int(k)] ...
def truncate_name(name, rule=None)
shorten taxa names for tree display Options of rule. This only affects tree display. - headn (eg. head3 truncates first 3 chars) - oheadn (eg. ohead3 retains only the first 3 chars) - tailn (eg. tail3 truncates last 3 chars) - otailn (eg. otail3 retains only the last 3 chars) n = 1 ~ 99
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1.812523
1.09882
for barcode in barcodemap: if barcode in name: return barcodemap[barcode] return name
def decode_name(name, barcodemap)
rename seq/taxon name, typically for a tree display, according to a barcode map given in a dictionary By definition barcodes should be distinctive.
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4.137232
0.820055
a, b = .1, .6 # Correspond to 200mya and 0mya def cv(x): return b - (x - b) / (maxx - minx) * (b - a) ax.plot((a, b), (.5, .5), "k-") tick = .015 for mya in xrange(maxx - 25, 0, -25): p = cv(mya) ax.plot((p, p), (.5, .5 - tick), "k-") ax.text(p, .5 - 2.5 * tick, str(mya...
def draw_geoscale(ax, minx=0, maxx=175)
Draw geological epoch on million year ago (mya) scale.
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4.508296
1.076382
p = OptionParser(main.__doc__) p.add_option("--outgroup", help="Outgroup for rerooting the tree. " + "Use comma to separate multiple taxa.") p.add_option("--noreroot", default=False, action="store_true", help="Don't reroot the input tree [default: %default]") p.add...
def main(args)
%prog newicktree Plot Newick formatted tree. The gene structure can be plotted along if --gffdir is given. The gff file needs to be `genename.gff`. If --sizes is on, also show the number of amino acids. With --barcode a mapping file can be provided to convert seq names to eg. species names, useful...
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2.849976
1.049982
cl = TreeFixCommandline(input=input, \ stree_file=stree_file, smap_file=smap_file, a_ext=a_ext, \ o=o_ext, n=n_ext, **kwargs) outtreefile = input.rsplit(o_ext, 1)[0] + n_ext print("TreeFix:", cl, file=sys.stderr) r, e = cl.run() if e: print("***TreeFix could not...
def run_treefix(input, stree_file, smap_file, a_ext=".fasta", \ o_ext=".dnd", n_ext = ".treefix.dnd", **kwargs)
get the ML tree closest to the species tree
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3.277595
1.000853
cl = GblocksCommandline(aln_file=align_fasta_file, **kwargs) r, e = cl.run() print("Gblocks:", cl, file=sys.stderr) if e: print("***Gblocks could not run", file=sys.stderr) return None else: print(r, file=sys.stderr) alignp = re.sub(r'.*Gblocks alignment:.*\(([...
def run_gblocks(align_fasta_file, **kwargs)
remove poorly aligned positions and divergent regions with Gblocks
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5.155605
1.036632
cl = FfitchCommandline(datafile=distfile, outtreefile=outtreefile, \ intreefile=intreefile, **kwargs) r, e = cl.run() if e: print("***ffitch could not run", file=sys.stderr) return None else: print("ffitch:", cl, file=sys.stderr) return outtreefile
def run_ffitch(distfile, outtreefile, intreefile=None, **kwargs)
Infer tree branch lengths using ffitch in EMBOSS PHYLIP
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4.297816
0.949246
tree = Tree(treefile, format=format) leaves = [t.name for t in tree.get_leaves()][::-1] outgroup = [] for o in must_open(outgroupfile): o = o.strip() for leaf in leaves: if leaf[:len(o)] == o: outgroup.append(leaf) if outgroup: break ...
def smart_reroot(treefile, outgroupfile, outfile, format=0)
simple function to reroot Newick format tree using ete2 Tree reading format options see here: http://packages.python.org/ete2/tutorial/tutorial_trees.html#reading-newick-trees
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2.967949
0.945126
phy_file = op.join(work_dir, "work", "aln.phy") AlignIO.write(alignment, file(phy_file, "w"), "phylip-relaxed") phyml_cl = PhymlCommandline(cmd=PHYML_BIN("phyml"), input=phy_file, **kwargs) logging.debug("Building ML tree using PhyML: %s" % phyml_cl) stdout, stderr = phyml_cl() tree_file ...
def build_ml_phyml(alignment, outfile, work_dir=".", **kwargs)
build maximum likelihood tree of DNA seqs with PhyML
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3.708928
1.035947
work_dir = op.join(work_dir, "work") mkdir(work_dir) phy_file = op.join(work_dir, "aln.phy") AlignIO.write(alignment, file(phy_file, "w"), "phylip-relaxed") raxml_work = op.abspath(op.join(op.dirname(phy_file), "raxml_work")) mkdir(raxml_work) raxml_cl = RaxmlCommandline(cmd=RAXML_BIN(...
def build_ml_raxml(alignment, outfile, work_dir=".", **kwargs)
build maximum likelihood tree of DNA seqs with RAxML
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3.248412
1.012152
assert op.isfile(reftree) shout = must_open(shout, "a") raxml_work = op.abspath(op.join(op.dirname(phy_file), "raxml_work")) mkdir(raxml_work) raxml_cl = RaxmlCommandline(cmd=RAXML_BIN("raxmlHPC"), \ sequences=phy_file, algorithm="h", model="GTRGAMMA", \ name="SH", starting_tree=reftre...
def SH_raxml(reftree, querytree, phy_file, shout="SH_out.txt")
SH test using RAxML querytree can be a single tree or a bunch of trees (eg. from bootstrapping)
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4.599276
0.996455
aln = AlignIO.read(alnfle, alntype) alnlen = aln.get_alignment_length() nseq = len(aln) subaln = None subalnfile = alnfle.rsplit(".", 1)[0] + "_{0}.{1}".format(subtype, alntype) if subtype == "synonymous": for j in range( 0, alnlen, 3 ): aa = None for i in r...
def subalignment(alnfle, subtype, alntype="fasta")
Subset synonymous or fourfold degenerate sites from an alignment input should be a codon alignment
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1.96585
1.070754
fw = must_open(filename+".merged", "w") rows = file(filename).readlines() rows = [row.strip().split(colsep) for row in rows] l = len(rows[0]) for rowi, row in enumerate(rows): n = len(rows) i = rowi+1 while i <= min(rowi+local, n-1): merge = 1 ro...
def merge_rows_local(filename, ignore=".", colsep="\t", local=10, \ fieldcheck=True, fsep=",")
merge overlapping rows within given row count distance
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2.420234
1.004121
tandems = [f.strip().split(",") for f in file(tandemfile)] fw = must_open(mcscanfile+".withtandems", "w") fp = must_open(mcscanfile) seen =set() for i, row in enumerate(fp): if row[0] == '#': continue anchorslist = row.strip().split("\t") anchors = set([a.spl...
def add_tandems(mcscanfile, tandemfile)
add tandem genes to anchor genes in mcscan file
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3.216578
1.023054
from jcvi.graphics.base import discrete_rainbow p = OptionParser(prepare.__doc__) p.add_option("--addtandem", help="path to tandemfile [default: %default]") p.add_option("--writecolors", default=False, action="store_true", \ help="generate a gene_name to color mapping file which will be ta...
def prepare(args)
%prog prepare mcscanfile cdsfile [options] Pick sequences from cdsfile to form fasta files, according to multiple alignment in the mcscanfile. The fasta sequences can then be used to construct phylogenetic tree. Use --addtandem=tandemfile to collapse tandems of anchors into single row. The tandemf...
3.817101
3.440197
1.109559
from jcvi.graphics.tree import draw_tree if shfile: SHs = DictFile(shfile, delimiter="\t") ntrees = len(trees) n = nrow * ncol for x in xrange(int(ceil(float(ntrees)/n))): fig = plt.figure(1, (iopts.w, iopts.h)) if iopts \ else plt.figure(1, (5, 5)) root ...
def _draw_trees(trees, nrow=1, ncol=1, rmargin=.3, iopts=None, outdir=".", shfile=None, **kwargs)
Draw one or multiple trees on one plot.
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2.812183
0.99605
p = OptionParser(enrich.__doc__) p.add_option("--ghost", default=False, action="store_true", help="Add ghost homologs already used [default: %default]") opts, args = p.parse_args(args) if len(args) != 3: sys.exit(not p.print_help()) omgfile, groupsfile, ntaxa = args ...
def enrich(args)
%prog enrich omgfile groups ntaxa > enriched.omg Enrich OMG output by pulling genes misses by OMG.
3.139113
2.95506
1.062284
from jcvi.formats.base import DictFile outfile = listfile.rsplit(".", 1)[0] + ".sorted.list" threadorder = thread.order fw = open(outfile, "w") lt = DictFile(listfile, keypos=column, valuepos=None) threaded = [] imported = set() for t in thread: accn = t.accn if acc...
def sort_layout(thread, listfile, column=0)
Sort the syntelog table according to chromomomal positions. First orient the contents against threadbed, then for contents not in threadbed, insert to the nearest neighbor.
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3.513239
1.025059
p = OptionParser(layout.__doc__) p.add_option("--sort", help="Sort layout file based on bedfile [default: %default]") opts, args = p.parse_args(args) if len(args) != 2: sys.exit(not p.print_help()) omgfile, taxa = args listfile = omgfile.rsplit(".", 1)[0] + ".list...
def layout(args)
%prog layout omgfile taxa Build column formatted gene lists after omgparse(). Use species list separated by comma in place of taxa, e.g. "BR,BO,AN,CN"
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3.118494
1.079622
p = OptionParser(omgparse.__doc__) opts, args = p.parse_args(args) if len(args) != 1: sys.exit(not p.print_help()) work, = args omgfiles = glob(op.join(work, "gf*.out")) for omgfile in omgfiles: omg = OMGFile(omgfile) best = omg.best() for bb in best: ...
def omgparse(args)
%prog omgparse work Parse the OMG outputs to get gene lists.
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2.884035
1.23769
p = OptionParser(group.__doc__) p.set_outfile() opts, args = p.parse_args(args) if len(args) < 1: sys.exit(not p.print_help()) anchorfiles = args groups = Grouper() for anchorfile in anchorfiles: ac = AnchorFile(anchorfile) for a, b, idx in ac.iter_pairs(): ...
def group(args)
%prog group anchorfiles Group the anchors into ortho-groups. Can input multiple anchor files.
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2.947951
1.152933
p = OptionParser(omg.__doc__) opts, args = p.parse_args(args) if len(args) < 1: sys.exit(not p.print_help()) weightsfiles = args groupfile = group(weightsfiles + ["--outfile=groups"]) weights = get_weights(weightsfiles) info = get_info() fp = open(groupfile) work =...
def omg(args)
%prog omg weightsfile Run Sankoff's OMG algorithm to get orthologs. Download OMG code at: <http://137.122.149.195/IsbraSoftware/OMGMec.html> This script only writes the partitions, but not launch OMGMec. You may need to: $ parallel "java -cp ~/code/OMGMec TestOMGMec {} 4 > {}.out" ::: work/gf????? ...
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4.224358
1.023512
from jcvi.formats.blast import cscore from jcvi.formats.base import DictFile p = OptionParser(omgprepare.__doc__) p.add_option("--norbh", action="store_true", help="Disable RBH hits [default: %default]") p.add_option("--pctid", default=0, type="int", help="Per...
def omgprepare(args)
%prog omgprepare ploidy anchorsfile blastfile Prepare to run Sankoff's OMG algorithm to get orthologs.
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3.051083
1.058566
p = OptionParser(tandem.__doc__) p.add_option("--tandem_Nmax", dest="tandem_Nmax", type="int", default=3, help="merge tandem genes within distance [default: %default]") p.add_option("--percent_overlap", type="int", default=50, help="tandem genes have >=x% aligned sequence,...
def tandem(args)
%prog tandem blast_file cds_file bed_file [options] Find tandem gene clusters that are separated by N genes, based on filtered blast_file by enforcing alignments between any two genes at least 50% (or user specified value) of either gene. pep_file can also be used in same manner.
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3.368327
1.073194
od = DefaultOrderedDict(list) if keep_attr_order else defaultdict(list) for name, value in parse_qsl(qs, keep_blank_values, strict_parsing): od[name].append(value) return od
def parse_qs(qs, keep_blank_values=0, strict_parsing=0, keep_attr_order=True)
Kind of like urlparse.parse_qs, except returns an ordered dict. Also avoids replicating that function's bad habit of overriding the built-in 'dict' type. Taken from below with modification: <https://bitbucket.org/btubbs/thumpy/raw/8cdece404f15/thumpy.py>
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2.917453
0.90538
'Find the position of an item. Raise ValueError if not found.' k = self._key(item) i = bisect_left(self._keys, k) j = bisect_right(self._keys, k) return self._items[i:j].index(item) + i
def index(self, item)
Find the position of an item. Raise ValueError if not found.
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2.824538
1.212153
'Insert a new item. If equal keys are found, add to the left' k = self._key(item) i = bisect_left(self._keys, k) self._keys.insert(i, k) self._items.insert(i, item)
def insert(self, item)
Insert a new item. If equal keys are found, add to the left
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2.620456
1.614497
'Insert a new item. If equal keys are found, add to the right' k = self._key(item) i = bisect_right(self._keys, k) self._keys.insert(i, k) self._items.insert(i, item)
def insert_right(self, item)
Insert a new item. If equal keys are found, add to the right
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2.625476
1.505586
'Remove first occurence of item. Raise ValueError if not found' i = self.index(item) del self._keys[i] del self._items[i]
def remove(self, item)
Remove first occurence of item. Raise ValueError if not found
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4.028533
1.220016
'Return first item with a key >= equal to item. Raise ValueError if not found' k = self._key(item) i = bisect_left(self._keys, k) if i != len(self): return self._items[i] raise ValueError('No item found with key at or above: %r' % (k,))
def find_ge(self, item)
Return first item with a key >= equal to item. Raise ValueError if not found
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3.196421
1.349439
'Return first item with a key > item. Raise ValueError if not found' k = self._key(item) i = bisect_right(self._keys, k) if i != len(self): return self._items[i] raise ValueError('No item found with key above: %r' % (k,))
def find_gt(self, item)
Return first item with a key > item. Raise ValueError if not found
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3.284884
1.358543
from jcvi.apps.grid import MakeManager p = OptionParser(batch.__doc__) opts, args = p.parse_args(args) if len(args) < 2: sys.exit(not p.print_help()) cdsfile = args[0] anchors = args[1:] workdirs = [".".join(op.basename(x).split(".")[:2]) for x in anchors] for wd in workd...
def batch(args)
%prog batch all.cds *.anchors Compute Ks values for a set of anchors file. This will generate a bunch of work directories for each comparisons. The anchorsfile should be in the form of specie1.species2.anchors.
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3.138587
1.12103