Archive 4 checkpoints (1-4)
Browse files- paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5nt/per_donor.csv +26 -0
- paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5nt/summary.json +537 -0
- paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5rt/README.md +73 -0
- paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5rt/contrast.csv +14 -0
paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5nt/per_donor.csv
ADDED
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| 1 |
+
arm,donor,group,n_generated_cells,n_observed_cells,n_reference_cells,gen_T cell,obs_T cell,gen_B cell,obs_B cell,gen_Monocyte,obs_Monocyte,gen_NK cell,obs_NK cell,gen_DC,obs_DC,gen_Other,obs_Other,comp_l1,comp_l1_obs_loo_null,pb_r,pb_own_rank,pb_top1,precision,coverage,precision_depth_matched,coverage_depth_matched,mono_pct_gen,mono_pct_obs,median_umi_gen,median_umi_obs
|
| 2 |
+
scsemi5nt,N064_TOL,TOL,1500,2498,150,0.34,0.6517213771016813,0.030666666666666665,0.044035228182546036,0.262,0.1677341873498799,0.262,0.07606084867894315,0.07133333333333333,0.010008006405124099,0.034,0.05044035228182546,0.6830605817987724,0.3779557749494401,0.7965994264064642,20,False,0.9533333333333334,0.7933333333333333,0.9533333333333334,0.29333333333333333,26.200000000000003,16.77341873498799,1026.0,662.0
|
| 3 |
+
scsemi5nt,N064_FU1,FU1,1500,4659,150,0.6253333333333333,0.6396222365314446,0.06533333333333333,0.016527151749302426,0.20133333333333334,0.13865636402661516,0.058,0.19167203262502683,0.008666666666666666,0.00472204335694355,0.04133333333333333,0.008800171710667526,0.2959218716462761,0.3574146776605954,0.9091359908129713,18,False,0.9533333333333334,0.6733333333333333,0.9866666666666667,0.8333333333333334,20.133333333333333,13.865636402661515,1026.5,925.0
|
| 4 |
+
scsemi5nt,N038_BC,BC,1500,2801,150,0.342,0.5608711174580507,0.03266666666666666,0.0046411995715815784,0.2833333333333333,0.16565512317029632,0.27266666666666667,0.2170653338093538,0.05466666666666667,0.034273473759371655,0.014666666666666666,0.017493752231345947,0.44339640604545993,0.2990892646419849,0.8904795968508609,3,False,1.0,0.54,0.96,0.6066666666666667,28.333333333333332,16.565512317029633,1026.0,1317.0
|
| 5 |
+
scsemi5nt,N038_AC,AC,1500,3976,150,0.55,0.6830985915492958,0.09733333333333333,0.00829979879275654,0.2,0.1290241448692153,0.046,0.12072434607645875,0.016666666666666666,0.03194164989939638,0.09,0.026911468812877263,0.44619584171696836,0.4382292825861123,0.9447971908212487,1,True,0.9866666666666667,0.7,0.9266666666666666,0.8333333333333334,20.0,12.90241448692153,1027.0,910.5
|
| 6 |
+
scsemi5nt,N055_AC,AC,1500,4308,150,0.362,0.340297121634169,0.078,0.07799442896935933,0.4513333333333333,0.32474466109563604,0.08133333333333333,0.2051996285979573,0.008666666666666666,0.0060352831940575676,0.018666666666666668,0.0457288765088208,0.30185701021355615,0.3381802929616731,0.9294787563298493,2,False,0.98,0.6333333333333333,0.9333333333333333,0.86,45.13333333333333,32.47446610956361,1028.0,1139.5
|
| 7 |
+
scsemi5nt,N136_TOL,TOL,1500,759,150,0.462,0.48616600790513836,0.0,0.10013175230566534,0.318,0.19104084321475626,0.0006666666666666666,0.0922266139657444,0.0,0.0013175230566534915,0.21933333333333332,0.12911725955204217,0.43435046113306985,0.3267042557350408,0.6901563147067785,7,False,0.6733333333333333,0.5266666666666666,1.0,0.3,31.8,19.104084321475625,1026.0,1837.0
|
| 8 |
+
scsemi5nt,N136_FU1,FU1,1500,2927,150,0.5593333333333333,0.4246668944311582,0.0026666666666666666,0.14724974376494704,0.368,0.19337205329689103,0.0006666666666666666,0.09531943969935087,0.0013333333333333333,0.0023915271609156134,0.068,0.13700034164673727,0.6185887712105683,0.43670640284299445,0.7843508885502634,1,True,0.5,0.5666666666666667,0.9466666666666667,0.47333333333333333,36.8,19.3372053296891,1027.0,1501.0
|
| 9 |
+
scsemi5nt,N128_BC,BC,1500,3902,150,0.3333333333333333,0.5158892875448488,0.038,0.06817016914402871,0.278,0.29420809841107126,0.238,0.07842132239876987,0.05466666666666667,0.03331624807790876,0.058,0.00999487442337263,0.4578684435332308,0.237517218766578,0.8736601869983515,2,False,0.9866666666666667,0.68,0.96,0.5133333333333333,27.800000000000004,29.420809841107125,1025.0,1088.0
|
| 10 |
+
scsemi5nt,N128_AC,AC,1500,2713,150,0.3333333333333333,0.5488389237007003,0.042,0.06524143015112421,0.2946666666666667,0.2790269074824917,0.238,0.07077036490969407,0.05733333333333333,0.029487652045705862,0.034666666666666665,0.006634721710283818,0.47749404103698245,0.2595394391709072,0.8551675181242036,13,False,0.9933333333333333,0.5466666666666666,0.9666666666666667,0.41333333333333333,29.46666666666667,27.90269074824917,1027.0,1181.0
|
| 11 |
+
scsemi5nt,N129_BC,BC,1500,3087,150,0.39,0.5963718820861678,0.025333333333333333,0.11305474570780694,0.294,0.08357628765792031,0.19066666666666668,0.18140589569160998,0.054,0.013281503077421444,0.046,0.012309685779073534,0.5881865889212828,0.3871201592227091,0.823584672733162,7,False,0.92,0.5733333333333334,0.8866666666666667,0.46,29.4,8.35762876579203,1026.0,1059.0
|
| 12 |
+
scsemi5nt,N129_AC,AC,1500,2798,150,0.344,0.7076483202287348,0.034666666666666665,0.04932094353109364,0.2793333333333333,0.07969978556111508,0.25066666666666665,0.13366690493209435,0.07,0.0228734810578985,0.021333333333333333,0.006790564689063617,0.7566051941863237,0.4740323616378469,0.856885500816245,8,False,1.0,0.7866666666666666,0.96,0.6,27.933333333333334,7.969978556111508,1025.0,759.0
|
| 13 |
+
scsemi5nt,N147_TOL,TOL,1500,3210,150,0.6393333333333333,0.44205607476635517,0.074,0.00809968847352025,0.19533333333333333,0.4193146417445483,0.056666666666666664,0.07102803738317758,0.018,0.0065420560747663555,0.016666666666666666,0.0529595015576324,0.5492710280373831,0.41393483656294416,0.8966846898296186,9,False,0.9733333333333334,0.42,0.98,0.7933333333333333,19.53333333333333,41.93146417445483,1027.0,1461.5
|
| 14 |
+
scsemi5nt,N158_AC,AC,1500,3749,150,0.6513333333333333,0.24619898639637236,0.08,0.026673779674579887,0.174,0.6295012003200854,0.036,0.04934649239797279,0.024666666666666667,0.024806615097359295,0.034,0.0234729261136303,0.9379752822975017,0.8296165589099086,0.8711982400440846,10,False,0.9933333333333333,0.7533333333333333,0.9866666666666667,0.5066666666666667,17.4,62.95012003200854,1025.5,1047.0
|
| 15 |
+
scsemi5nt,N159_BC,BC,1500,3779,150,1.0,0.6996559936491136,0.0,0.018523418893887273,0.0,0.08123842286319131,0.0,0.173590897062715,0.0,0.014024874305371792,0.0,0.01296639322572109,0.600688012701773,0.44104315672042127,0.7356978188014487,1,True,0.4,0.56,1.0,0.41333333333333333,0.0,8.123842286319132,1024.5,1045.0
|
| 16 |
+
scsemi5nt,N001_TOL,TOL,1500,5658,150,0.5913333333333334,0.51926475786497,0.108,0.03375751148815836,0.182,0.21067515022976316,0.04466666666666667,0.2163308589607635,0.032,0.003711558854718982,0.042,0.016260162601626018,0.4006786850477201,0.1319697438356509,0.9276257548956623,15,False,0.9733333333333334,0.68,0.98,0.6666666666666666,18.2,21.067515022976316,1026.5,787.5
|
| 17 |
+
scsemi5nt,N001_FU1,FU1,1500,4547,150,0.2986666666666667,0.29601935341983726,0.030666666666666665,0.06575764240158347,0.434,0.48823400043985044,0.108,0.13591378931163403,0.05333333333333334,0.0010996261271167802,0.07533333333333334,0.012975588299978007,0.2344775309728026,0.5380476132456627,0.8084269756763868,7,False,0.9666666666666667,0.6866666666666666,0.8733333333333333,0.23333333333333334,43.4,48.823400043985046,1028.0,1165.0
|
| 18 |
+
scsemi5nt,N002_TOL,TOL,1500,6318,150,0.334,0.41579613801836024,0.036,0.05777144666033555,0.246,0.3779677113010446,0.272,0.1179170623615068,0.094,0.020101297879075657,0.018,0.010446343779677113,0.47107059195948087,0.3320124203615185,0.8520269647638552,12,False,1.0,0.6866666666666666,0.98,0.34,24.6,37.79677113010446,1025.0,933.5
|
| 19 |
+
scsemi5nt,N002_FU1,FU1,1500,4975,150,0.3466666666666667,0.31618090452261305,0.042,0.07276381909547738,0.2793333333333333,0.45587939698492463,0.25466666666666665,0.12261306532663317,0.066,0.02271356783919598,0.011333333333333334,0.00984924623115578,0.41461976549413737,0.5230450765198029,0.8739902116944046,17,False,0.98,0.5066666666666667,0.9733333333333334,0.26666666666666666,27.933333333333334,45.58793969849246,1026.0,979.0
|
| 20 |
+
scsemi5nt,N005_BC,BC,1500,6359,150,0.32466666666666666,0.7200817738638151,0.037333333333333336,0.05016512030193427,0.302,0.11448340934109137,0.26466666666666666,0.09891492373014625,0.05466666666666667,0.007705614090265765,0.016666666666666666,0.008649158672747287,0.8164937883314988,0.4953276467168827,0.9096490339171518,4,False,0.9466666666666667,0.8133333333333334,0.92,0.7866666666666666,30.2,11.448340934109137,1027.0,782.0
|
| 21 |
+
scsemi5nt,N005_AC,AC,1500,3433,150,0.49,0.8100786484124672,0.018666666666666668,0.015147101660355374,0.19666666666666666,0.07952228371686572,0.154,0.08243518788231867,0.12733333333333333,0.010195164579085349,0.013333333333333334,0.0026216137489076608,0.6401572968249344,0.675321395814187,0.7969955332126598,13,False,0.7666666666666667,0.44666666666666666,0.9066666666666666,0.25333333333333335,19.666666666666664,7.952228371686572,1025.0,1126.0
|
| 22 |
+
scsemi5nt,N022_TOL,TOL,1500,3651,150,0.36733333333333335,0.4864420706655711,0.06933333333333333,0.03697617091207888,0.462,0.045740892906053134,0.07466666666666667,0.4182415776499589,0.008,0.0,0.018666666666666668,0.01259928786633799,0.92536729663106,0.5320299851087325,0.8034120361715894,22,False,0.9066666666666666,0.37333333333333335,0.8,0.35333333333333333,46.2,4.574089290605313,1027.0,1410.0
|
| 23 |
+
scsemi5nt,N022_FU1,FU1,1500,2463,150,0.35133333333333333,0.48477466504263095,0.021333333333333333,0.048721071863580996,0.26266666666666666,0.20259845716605765,0.2846666666666667,0.25334957369062117,0.05266666666666667,0.0,0.027333333333333334,0.010556232237109216,0.3216581404790906,0.20224597719005705,0.8154682395836624,18,False,0.9666666666666667,0.44,0.96,0.28,26.266666666666666,20.259845716605763,1026.0,1459.0
|
| 24 |
+
scsemi5nt,N035_TOL,TOL,1500,1598,150,0.6026666666666667,0.2853566958698373,0.03266666666666666,0.016896120150187734,0.156,0.31101376720901125,0.146,0.3404255319148936,0.04466666666666667,0.01376720901126408,0.018,0.03254067584480601,0.7279599499374217,0.5092117579012594,0.9150175210618444,8,False,0.98,0.43333333333333335,0.9733333333333334,0.48,15.6,31.101376720901126,1025.0,1855.5
|
| 25 |
+
scsemi5nt,N043_TOL,TOL,1500,2074,150,0.5713333333333334,0.3548698167791707,0.036,0.05737704918032787,0.17133333333333334,0.04580520732883317,0.16666666666666666,0.4811957569913211,0.046,0.0028929604628736743,0.008666666666666666,0.05785920925747348,0.7701973641915784,0.728022429812273,0.8946557712343053,23,False,0.92,0.4,0.9666666666666667,0.5733333333333334,17.133333333333333,4.580520732883317,1025.0,1315.5
|
| 26 |
+
scsemi5nt,N043_FU1,FU1,1500,3437,150,0.5126666666666667,0.1638056444573756,0.016,0.06779167878964212,0.38266666666666665,0.5728833284841431,0.048,0.14896712249054408,0.0,0.0034914169333721268,0.04066666666666666,0.043060808844922895,0.6977220444185821,0.7356501997739635,0.7638277079142118,14,False,0.46,0.36,0.8933333333333333,0.08666666666666667,38.266666666666666,57.28833284841431,1025.0,1422.0
|
paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5nt/summary.json
ADDED
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@@ -0,0 +1,537 @@
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|
| 1 |
+
{
|
| 2 |
+
"schema": "paired_ft_scores.v1",
|
| 3 |
+
"dataset": "ji2025_chili",
|
| 4 |
+
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|
| 5 |
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|
| 6 |
+
"/home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/tools/score_ft.py",
|
| 7 |
+
"--dataset",
|
| 8 |
+
"ji2025_chili",
|
| 9 |
+
"--out",
|
| 10 |
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|
| 11 |
+
"--threads",
|
| 12 |
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"6",
|
| 13 |
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"--donors",
|
| 14 |
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"/home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/data/ji2025_chili/fewshot_n5/draw0/test_samples.txt",
|
| 15 |
+
"--case-groups",
|
| 16 |
+
"TOL,FU1",
|
| 17 |
+
"--control-groups",
|
| 18 |
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"BC,AC",
|
| 19 |
+
"--arm",
|
| 20 |
+
"scsemi5nt=/home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/generated/ji2025_chili/fewshot_n5/draw0/scsemi5nt__raw.npz"
|
| 21 |
+
],
|
| 22 |
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|
| 23 |
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"scsemi5nt"
|
| 24 |
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|
| 25 |
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|
| 26 |
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"N064_TOL",
|
| 27 |
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|
| 28 |
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"N038_BC",
|
| 29 |
+
"N038_AC",
|
| 30 |
+
"N055_AC",
|
| 31 |
+
"N136_TOL",
|
| 32 |
+
"N136_FU1",
|
| 33 |
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"N128_BC",
|
| 34 |
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"N128_AC",
|
| 35 |
+
"N129_BC",
|
| 36 |
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"N129_AC",
|
| 37 |
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"N147_TOL",
|
| 38 |
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"N158_AC",
|
| 39 |
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"N159_BC",
|
| 40 |
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"N001_TOL",
|
| 41 |
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"N001_FU1",
|
| 42 |
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"N002_TOL",
|
| 43 |
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"N002_FU1",
|
| 44 |
+
"N005_BC",
|
| 45 |
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"N005_AC",
|
| 46 |
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"N022_TOL",
|
| 47 |
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"N022_FU1",
|
| 48 |
+
"N035_TOL",
|
| 49 |
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"N043_TOL",
|
| 50 |
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"N043_FU1"
|
| 51 |
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|
| 52 |
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|
| 53 |
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|
| 54 |
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| 55 |
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| 56 |
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| 60 |
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| 62 |
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| 63 |
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| 64 |
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| 66 |
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| 69 |
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| 71 |
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| 72 |
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| 73 |
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| 74 |
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| 75 |
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| 76 |
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| 77 |
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|
| 78 |
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|
| 79 |
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|
| 80 |
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| 81 |
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| 82 |
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| 83 |
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| 84 |
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| 85 |
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| 86 |
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| 87 |
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| 92 |
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| 114 |
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| 116 |
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| 117 |
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| 118 |
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| 119 |
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| 137 |
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| 140 |
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| 141 |
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| 142 |
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| 143 |
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|
| 144 |
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|
| 145 |
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|
| 146 |
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|
| 147 |
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|
| 148 |
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|
| 149 |
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|
| 150 |
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|
| 151 |
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|
| 152 |
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| 153 |
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| 154 |
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| 155 |
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| 156 |
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| 157 |
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| 158 |
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|
| 159 |
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| 160 |
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| 161 |
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| 162 |
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| 163 |
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| 164 |
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| 165 |
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| 166 |
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| 167 |
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| 168 |
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| 169 |
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| 170 |
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| 171 |
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| 172 |
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|
| 173 |
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|
| 174 |
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"enabled": true,
|
| 175 |
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"score": "top-50 DEG overlap per cell type (benchmark total_top50_overlap, Welch backend)",
|
| 176 |
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"source": "/home/sj_server_1/PBISC/immun-pbmc-fm/bulk2sc-paper/scripts/evaluate_generated_vs_truth.py",
|
| 177 |
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"functions": [
|
| 178 |
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|
| 179 |
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|
| 180 |
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|
| 181 |
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],
|
| 182 |
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|
| 183 |
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|
| 184 |
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|
| 185 |
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|
| 186 |
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|
| 187 |
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|
| 188 |
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|
| 189 |
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|
| 190 |
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|
| 191 |
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|
| 192 |
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|
| 193 |
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| 194 |
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| 195 |
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|
| 196 |
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|
| 197 |
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|
| 198 |
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|
| 199 |
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|
| 200 |
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|
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|
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|
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|
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|
| 205 |
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|
| 206 |
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|
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|
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|
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|
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|
| 213 |
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|
| 214 |
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|
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|
| 221 |
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|
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|
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|
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|
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|
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|
| 229 |
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|
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|
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|
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|
| 237 |
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|
| 238 |
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|
| 239 |
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|
| 240 |
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},
|
| 241 |
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"headline": {
|
| 242 |
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paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5rt/README.md
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| 1 |
+
# score_ft.py output: ji2025_chili, 25 scored donors
|
| 2 |
+
|
| 3 |
+
Arms: scsemi5rt. Every arm is scored on the same donors against the same observed reference.
|
| 4 |
+
|
| 5 |
+
## Inputs and fixed contracts
|
| 6 |
+
- Observed reference: `/home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/scoring_cache/ji2025_chili/reference.npz` (sha 19c30f56db69), built from datadir:/home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/data/ji2025_chili;
|
| 7 |
+
observed cells sum exactly to the features pseudobulk of every donor. Observed labels: /home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/scoring_cache/ji2025_chili/observed_labels/labels/labels.parquet.
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| 8 |
+
- Generated cells: `generated_counts` read out once with Poisson(seed 20260916) over the **whole NPZ in row order**
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| 9 |
+
(`poisson_readout.poisson_integerise`), then the scored donors' rows. These integer counts are used for labels, embedding,
|
| 10 |
+
depth and the contrast. Pseudobulk uses the summed `generated_counts` (rates), as `score_generated.py`.
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| 11 |
+
- Labels: frozen f01common scANVI (`predict_scanvi.py`, CPU, zero query epochs), six-type gate ['T cell', 'B cell', 'Monocyte', 'NK cell', 'DC', 'Other']; query genes =
|
| 12 |
+
the 1,006-gene annotation list minus genes the dataset's sc assay did not measure in every sample (1004 genes).
|
| 13 |
+
- Scoring genes: 6k panel genes measured by both assays in every sample of the dataset (5752 genes).
|
| 14 |
+
|
| 15 |
+
## Definitions
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| 16 |
+
1. **Composition**: fraction of each donor's cells per six-type label (all generated cells; all observed QC cells).
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| 17 |
+
`comp_l1` = sum over the six labels of |generated - observed| (immicro_common.l1). `comp_l1_obs_loo_null` = L1 of the
|
| 18 |
+
donor's observed composition to the mean of the *other scored donors'* observed compositions. `composition_top1` =
|
| 19 |
+
fraction of donors whose generated composition is closest (L1) to their own observed composition.
|
| 20 |
+
2. **Pseudobulk**: `pb_r` = Pearson r of CPM-log1p(summed generated_counts) vs CPM-log1p(observed pseudobulk) on the scoring
|
| 21 |
+
genes (evaluate_realbulk.cpm_log1p / paired_r). `pb_top1` = the donor's generated pseudobulk is closest (Pearson) to its own
|
| 22 |
+
observed pseudobulk among the 25 scored donors (immicro_common.retrieval = evaluate_realbulk.top1 arithmetic).
|
| 23 |
+
3. **Precision / coverage / density** (stage_b_embed.py functions): per-cell CP10K over the scoring genes + log1p; PCA-50
|
| 24 |
+
(randomized, random_state 20260925) and k = 10 real-real k-NN balls fitted on **R1 of the scored donors only**.
|
| 25 |
+
Reference rule (independent of fold and arm): per donor, cells in canonical order (library_id+barcode), one
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| 26 |
+
permutation from `default_rng([20260925, uint64(sha256(donor)[:8])])`; R1 = first n, R2 = next n,
|
| 27 |
+
n = min(150, n_cells // 2) (3750 R1 cells here). Generated query: per donor n cells (the donor's R1 size),
|
| 28 |
+
without replacement, `default_rng([20260926, same key])`. Precision = fraction of generated cells inside any R1 ball
|
| 29 |
+
(Kynkaanniemi 2019); coverage = fraction of R1 cells whose ball holds a generated cell; density = Naeem 2020.
|
| 30 |
+
Per-donor precision = that donor's generated cells; per-donor coverage = that donor's R1 cells (the pooled values are
|
| 31 |
+
their n-weighted means; plain means when every donor has 150 reference cells). R2 (held-out real cells of the same donors) is the
|
| 32 |
+
perfect-generator control: precision 0.9960, coverage 1.0000.
|
| 33 |
+
**Depth matched**: stage_b_embed depth matching run pairwise (R1 vs this arm): per donor and within-donor rank of detected
|
| 34 |
+
genes, both cells are thinned by UMI sampling without replacement to the smaller detected-gene count; PCA kept from
|
| 35 |
+
un-thinned R1, radii recomputed on thinned R1; fresh rng(20260929) per arm. R2 control: precision
|
| 36 |
+
0.9957, coverage 1.0000.
|
| 37 |
+
4. **Monocyte %** = 100 x Monocyte fraction (all cells) per donor; **median UMI per cell** = median over cells of the
|
| 38 |
+
Poisson-read (generated) / observed counts summed over the scoring genes (per donor, and pooled).
|
| 39 |
+
5. **Group contrast** (T, B, Monocyte, NK): case groups ['TOL', 'FU1'] (n = 14) vs control groups ['BC', 'AC'] (n = 11); rule: explicit --case-groups / --control-groups; groups from features groups.
|
| 40 |
+
Genes per type = scoring genes detected (count > 0) in >= 1 % of the scored donors' observed
|
| 41 |
+
cells of that type. Primary statistic `mean_cp10k`: per-cell CP10K over the scoring genes; per donor the mean over its
|
| 42 |
+
cells of the type (donors with >= 1 such cell); group mean = mean over the group's donors (donor-balanced);
|
| 43 |
+
logFC = log2(case mean + 1) - log2(control mean + 1). Secondary `pseudobulk_log1p_cpm`: the SLE benchmark evaluator's
|
| 44 |
+
statistic (donor x type summed counts, log1p CPM over the scoring genes, mean case - mean control;
|
| 45 |
+
evaluate_generated_vs_truth.group_log_fold_change). Both are computed on the generated cells (their frozen labels) and on
|
| 46 |
+
the observed cells; `r_generated` = Pearson r of the two logFC vectors. Broadcast baseline `r_broadcast`: the same
|
| 47 |
+
observed vector against the logFC of the input bulk (condition NPZ `bulk_counts` of the arm, scoring genes, per-donor
|
| 48 |
+
CP10K resp. log1p CPM, group means). `partial_r_generated_given_broadcast`: r of the OLS residuals of observed and
|
| 49 |
+
generated logFC on the bulk logFC (residual_specificity.py).
|
| 50 |
+
6. **Top-50 DEG overlap** (contrast.csv rows `statistic = welch_top50_deg`; summary `deg_top50`): the SLE benchmark's score (`total_top50_overlap` of
|
| 51 |
+
`bulk2sc-paper/scripts/evaluate_generated_vs_truth.py`, Welch backend: the "top-50 DEG 81 / 84" of the final model), run
|
| 52 |
+
with the evaluator's own functions on the same donor x type pseudobulks as the contrast. Per cell type, observed and
|
| 53 |
+
generated separately: donor x type summed counts on all 5752 scoring genes (donors of the two groups with
|
| 54 |
+
>= 1 cell of the type) -> `welch_bh_deg` (log1p CPM, two-sided Welch t-test case vs control, Benjamini-Hochberg over the
|
| 55 |
+
genes with a defined p-value; needs >= 2 donors per group) -> `top_deg_genes` (FDR < 0.05, descending |logFC|,
|
| 56 |
+
first 50; shorter when fewer genes pass). `top50_overlap` = |observed list & generated list|; `top50_jaccard`
|
| 57 |
+
the evaluator's Jaccard. Summary `sum` = total over the scored types (the quantity reported as 81), `max` = the sum of
|
| 58 |
+
the observed list lengths (the "of 262"), `sum_4type` = T + B + Monocyte + NK. A type is scored when its observed test
|
| 59 |
+
is defined; an arm whose generated test is undefined for a scored type gets overlap 0. Types: T cell, B cell, Monocyte, NK cell, DC
|
| 60 |
+
(aggregates: /home/sj_server_1/PBISC/immun-pbmc-fm/model_8_sparse_data_diffusion/plans/paired_finetune_intradataset_20260927/scoring_cache/ji2025_chili/deg_reference.npz).
|
| 61 |
+
**Broadcast analogue** (`top50_overlap_broadcast`, as `sle_broadcast.py` `broadcast_input_bulk`; not part of the
|
| 62 |
+
published benchmark): the same test on the arm's condition bulk_counts (scoring genes, the 14 + 11
|
| 63 |
+
donors), one whole-PBMC top-50 list, compared with every type's observed list.
|
| 64 |
+
|
| 65 |
+
## Files
|
| 66 |
+
- `per_donor.csv`: one row per arm x donor (all per-donor quantities above).
|
| 67 |
+
- `summary.json`: per arm the donor means / pooled values, provenance (NPZ sha, receipt check, generation metadata,
|
| 68 |
+
Poisson nnz of the whole NPZ, annotation contract), and the reference block (PCA, R2 control).
|
| 69 |
+
- `contrast.csv`: per arm x cell type x statistic (`mean_cp10k`, `pseudobulk_log1p_cpm`: logFC r; `welch_top50_deg`:
|
| 70 |
+
top-50 DEG overlap, T / B / Monocyte / NK / DC; columns of the other kind are empty).
|
| 71 |
+
- `arms/<arm>/labels/` (predict_scanvi.py labels + manifest), `arms/<arm>/generated_cells.parquet` (NPZ row, donor, label,
|
| 72 |
+
UMI on the scoring genes, P/C subsample flag), `arms/<arm>/deg_top50_lists.csv` (observed, generated and broadcast
|
| 73 |
+
top-50 gene lists, Ensembl ids, rank order).
|
paper-releases/pbisc-diffusion/updates/20261003T174003Z-scsemi-readout-sensitivity/diffusion/dataset/server_1/model8/plans/paired_finetune_intradataset_20260927/scores/ji2025_chili/fewshot_n5/draw0_scsemi5rt/contrast.csv
ADDED
|
@@ -0,0 +1,14 @@
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|
| 1 |
+
arm,celltype,statistic,n_genes,r_generated,r_broadcast,partial_r_generated_given_broadcast,r_generated_vs_broadcast,n_case_donors_obs,n_control_donors_obs,n_case_donors_gen,n_control_donors_gen,n_cells_obs,n_cells_gen,deg_scored,top50_overlap,top50_jaccard,obs_top_list_size,gen_top_list_size,n_sig_obs,n_sig_gen,top50_overlap_broadcast,top50_jaccard_broadcast,broadcast_top_list_size,n_sig_broadcast
|
| 2 |
+
scsemi5rt,T cell,mean_cp10k,3038,0.11547474985640313,0.21197028589936057,0.039405682318534134,0.3764562875510768,14,11,14,11,44851,19077,,,,,,,,,,,
|
| 3 |
+
scsemi5rt,T cell,pseudobulk_log1p_cpm,3038,0.13461070401829223,0.3111080127833571,0.05015548210410561,0.2858597632324631,14,11,14,11,44851,19077,,,,,,,,,,,
|
| 4 |
+
scsemi5rt,B cell,mean_cp10k,3233,-0.09244414035009216,0.10073938447302222,-0.11424074070033793,0.1900370278101038,14,11,11,9,4424,1145,,,,,,,,,,,
|
| 5 |
+
scsemi5rt,B cell,pseudobulk_log1p_cpm,3233,-0.052988838157227366,0.012072638770403557,-0.056411918699425995,0.19422446892651796,14,11,11,9,4424,1145,,,,,,,,,,,
|
| 6 |
+
scsemi5rt,Monocyte,mean_cp10k,3193,0.09487985600822,0.3732268158395985,-0.01357550029342054,0.2865449602730492,14,11,14,10,22847,8666,,,,,,,,,,,
|
| 7 |
+
scsemi5rt,Monocyte,pseudobulk_log1p_cpm,3193,0.11658901167032525,0.3506580485024196,0.0530267915839915,0.1935453435797445,14,11,14,10,22847,8666,,,,,,,,,,,
|
| 8 |
+
scsemi5rt,NK cell,mean_cp10k,3059,0.0608195729082749,0.1665379702415652,0.012175999873344655,0.29634630134051226,14,11,11,10,14219,4756,,,,,,,,,,,
|
| 9 |
+
scsemi5rt,NK cell,pseudobulk_log1p_cpm,3059,0.03426716129598247,0.15048321474174686,-0.0021445557668943152,0.24138640182875032,14,11,11,10,14219,4756,,,,,,,,,,,
|
| 10 |
+
scsemi5rt,T cell,welch_top50_deg,5752,,,,,14,11,14,11,44851,19077,True,0,0.0,50,0,1831,0,0,0.0,4,4
|
| 11 |
+
scsemi5rt,B cell,welch_top50_deg,5752,,,,,14,11,11,9,4424,1145,True,0,0.0,9,0,9,0,0,0.0,4,4
|
| 12 |
+
scsemi5rt,Monocyte,welch_top50_deg,5752,,,,,14,11,14,10,22847,8666,True,0,0.0,10,0,10,0,0,0.0,4,4
|
| 13 |
+
scsemi5rt,NK cell,welch_top50_deg,5752,,,,,14,11,11,10,14219,4756,True,0,0.0,50,0,358,0,0,0.0,4,4
|
| 14 |
+
scsemi5rt,DC,welch_top50_deg,5752,,,,,12,11,11,10,1176,1701,True,0,0.0,6,0,6,0,0,0.0,4,4
|