| We studied whole pancreatic islets from 5-week-old mice in which Klf4 was knocked out in the alpha-cell lineage. The `group` column holds the genotype: Klf4_alphaKO are Klf4fl/fl;Gcg-iCre knockout mice (samples Mut_1 to Mut_3) and control_Klf4wt are Klf4+/+;Gcg-iCre mice that carry only the Cre (samples Con_1 to Con_3). The islets were not sorted, so each sample contains all islet cell types. All islets were collected at the same age, so there is no time variable. | |
| Each sample is a separate whole-islet RNA preparation sequenced as one library, and there are no technical replicates. There is no record of repeated sampling of a mouse, pooling of mice or litter pairing; the numbers in the sample names only count the samples within each genotype. No islet-isolation, library or sequencing batch is recorded, so we have no batch information. The sex of the mice is not recorded, and no other variable matters to us; the remaining columns are constant annotations. | |
| The data are bulk poly(A) mRNA-seq (Tecan Universal Plus mRNA-seq). The matrix holds raw gene-level read counts from featureCounts (mm10), one row per gene. | |
| Our question is which genes are differentially expressed in Klf4 alpha-cell knockout islets (Klf4_alphaKO) compared with Cre-only control islets (control_Klf4wt), using all six samples. | |