| We studied the epidermis of mice with imiquimod-induced psoriasis-like dermatitis, comparing mice with an epidermis-specific conditional knockout of Hnrnp-u (Hnrnpu) with control mice. All eight mice were treated with imiquimod (`treatment` is "Imiquimod" for every sample) and sampled at one time point after the treatment, so time is not a variable here. The groups are in the `group` column, and `genotype` holds the same labels: "Hnrnp-u knockout" (4 mice, hnrnpu-cKO-1 to hnrnpu-cKO-4 in `sample_id`) and "WT" (4 control mice, WT-1 to WT-4). The exact genotype of the controls and the Cre line used are not recorded. | |
| Each sample is the total epidermal RNA of a different mouse, so no mouse contributes more than one sample, and there is no record linking samples with the same number across the two groups. Each sample was sequenced once, so there are no technical replicates. The submitter recorded all samples as one batch (`src_batch` is "same" for every sample), so there is no known batch variable. We have no other variable we consider relevant; the remaining columns are GEO annotations. | |
| The data are bulk RNA-seq (Illumina NovaSeq 6000). Reads were aligned with HISAT2 and counted with HTSeq-count, so the matrix holds raw gene-level read counts for 23,171 genes identified by gene symbols. | |
| Our question is which genes are differentially expressed in the epidermis of Hnrnp-u knockout mice ("Hnrnp-u knockout") compared with control mice ("WT") under imiquimod treatment, using all eight samples. | |