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def generateVariantAnnotation(self, variant): """ Generate a random variant annotation based on a given variant. This generator should be seeded with a value that is unique to the variant so that the same annotation will always be produced regardless of the order it is generated ...
def populateFromRow(self, annotationSetRecord): """ Populates this VariantAnnotationSet from the specified DB row. """ self._annotationType = annotationSetRecord.annotationtype self._analysis = protocol.fromJson( annotationSetRecord.analysis, protocol.Analysis) ...
def _getAnnotationAnalysis(self, varFile): """ Assembles metadata within the VCF header into a GA4GH Analysis object. :return: protocol.Analysis """ header = varFile.header analysis = protocol.Analysis() formats = header.formats.items() infos = header.inf...
def getVariantAnnotations(self, referenceName, startPosition, endPosition): """ Generator for iterating through variant annotations in this variant annotation set. :param referenceName: :param startPosition: :param endPosition: :return: generator of protocol.Varia...
def convertLocation(self, pos): """ Accepts a position string (start/length) and returns a GA4GH AlleleLocation with populated fields. :param pos: :return: protocol.AlleleLocation """ if isUnspecified(pos): return None coordLen = pos.split('/')...
def convertLocationHgvsC(self, hgvsc): """ Accepts an annotation in HGVS notation and returns an AlleleLocation with populated fields. :param hgvsc: :return: """ if isUnspecified(hgvsc): return None match = re.match(".*c.(\d+)(\D+)>(\D+)", hgvs...
def convertLocationHgvsP(self, hgvsp): """ Accepts an annotation in HGVS notation and returns an AlleleLocation with populated fields. :param hgvsp: :return: protocol.AlleleLocation """ if isUnspecified(hgvsp): return None match = re.match(".*p...
def addLocations(self, effect, protPos, cdnaPos): """ Adds locations to a GA4GH transcript effect object by parsing HGVS annotation fields in concert with and supplied position values. :param effect: protocol.TranscriptEffect :param protPos: String representing protein po...
def convertTranscriptEffect(self, annStr, hgvsG): """ Takes the ANN string of a SnpEff generated VCF, splits it and returns a populated GA4GH transcript effect object. :param annStr: String :param hgvsG: String :return: effect protocol.TranscriptEffect() """ ...
def convertSeqOntology(self, seqOntStr): """ Splits a string of sequence ontology effects and creates an ontology term record for each, which are built into an array of return soTerms. :param seqOntStr: :return: [protocol.OntologyTerm] """ return [ ...
def convertVariantAnnotation(self, record): """ Converts the specfied pysam variant record into a GA4GH variant annotation object using the specified function to convert the transcripts. """ variant = self._variantSet.convertVariant(record, []) annotation = self._...
def _attributeStr(self, name): """ Return name=value for a single attribute """ return "{}={}".format( _encodeAttr(name), ",".join([_encodeAttr(v) for v in self.attributes[name]]))
def _attributeStrs(self): """ Return name=value, semi-colon-separated string for attributes, including url-style quoting """ return ";".join([self._attributeStr(name) for name in self.attributes.iterkeys()])
def featureName(self): """ ID attribute from GFF3 or None if record doesn't have it. Called "Name" rather than "Id" within GA4GH, as there is no guarantee of either uniqueness or existence. """ featId = self.attributes.get("ID") if featId is not None: ...
def _linkFeature(self, feature): """ Link a feature with its parents. """ parentNames = feature.attributes.get("Parent") if parentNames is None: self.roots.add(feature) else: for parentName in parentNames: self._linkToParent(feature...
def _linkToParent(self, feature, parentName): """ Link a feature with its children """ parentParts = self.byFeatureName.get(parentName) if parentParts is None: raise GFF3Exception( "Parent feature does not exist: {}".format(parentName), ...
def linkChildFeaturesToParents(self): """ finish loading the set, constructing the tree """ # features maybe disjoint for featureParts in self.byFeatureName.itervalues(): for feature in featureParts: self._linkFeature(feature)
def _recSortKey(r): """ Sort order for Features, by genomic coordinate, disambiguated by feature type (alphabetically). """ return r.seqname, r.start, -r.end, r.type
def _writeRec(self, fh, rec): """ Writes a single record to a file provided by the filehandle fh. """ fh.write(str(rec) + "\n") for child in sorted(rec.children, key=self._recSortKey): self._writeRec(fh, child)
def write(self, fh): """ Write set to a GFF3 format file. :param file fh: file handle for file to write to """ fh.write(GFF3_HEADER+"\n") for root in sorted(self.roots, key=self._recSortKey): self._writeRec(fh, root)
def _open(self): """ open input file, optionally with decompression """ if self.fileName.endswith(".gz"): return gzip.open(self.fileName) elif self.fileName.endswith(".bz2"): return bz2.BZ2File(self.fileName) else: return open(self.file...
def _parseAttrVal(self, attrStr): """ Returns tuple of tuple of (attr, value), multiple are returned to handle multi-value attributes. """ m = self.SPLIT_ATTR_RE.match(attrStr) if m is None: raise GFF3Exception( "can't parse attribute/value: '"...
def _parseAttrs(self, attrsStr): """ Parse the attributes and values """ attributes = dict() for attrStr in self.SPLIT_ATTR_COL_RE.split(attrsStr): name, vals = self._parseAttrVal(attrStr) if name in attributes: raise GFF3Exception( ...
def _parseRecord(self, gff3Set, line): """ Parse one record. """ row = line.split("\t") if len(row) != self.GFF3_NUM_COLS: raise GFF3Exception( "Wrong number of columns, expected {}, got {}".format( self.GFF3_NUM_COLS, len(row)), ...
def parse(self): """ Run the parse and return the resulting Gff3Set object. """ fh = self._open() try: gff3Set = Gff3Set(self.fileName) for line in fh: self.lineNumber += 1 self._parseLine(gff3Set, line[0:-1]) finall...
def addDataset(self, dataset): """ Adds the specified dataset to this data repository. """ id_ = dataset.getId() self._datasetIdMap[id_] = dataset self._datasetNameMap[dataset.getLocalId()] = dataset self._datasetIds.append(id_)
def addReferenceSet(self, referenceSet): """ Adds the specified reference set to this data repository. """ id_ = referenceSet.getId() self._referenceSetIdMap[id_] = referenceSet self._referenceSetNameMap[referenceSet.getLocalId()] = referenceSet self._referenceSet...
def addOntology(self, ontology): """ Add an ontology map to this data repository. """ self._ontologyNameMap[ontology.getName()] = ontology self._ontologyIdMap[ontology.getId()] = ontology self._ontologyIds.append(ontology.getId())
def getPeer(self, url): """ Select the first peer in the datarepo with the given url simulating the behavior of selecting by URL. This is only used during testing. """ peers = filter(lambda x: x.getUrl() == url, self.getPeers()) if len(peers) == 0: raise excep...
def getDataset(self, id_): """ Returns a dataset with the specified ID, or raises a DatasetNotFoundException if it does not exist. """ if id_ not in self._datasetIdMap: raise exceptions.DatasetNotFoundException(id_) return self._datasetIdMap[id_]
def getDatasetByName(self, name): """ Returns the dataset with the specified name. """ if name not in self._datasetNameMap: raise exceptions.DatasetNameNotFoundException(name) return self._datasetNameMap[name]
def getOntology(self, id_): """ Returns the ontology with the specified ID. """ if id_ not in self._ontologyIdMap: raise exceptions.OntologyNotFoundException(id_) return self._ontologyIdMap[id_]
def getOntologyByName(self, name): """ Returns an ontology by name """ if name not in self._ontologyNameMap: raise exceptions.OntologyNameNotFoundException(name) return self._ontologyNameMap[name]
def getReferenceSet(self, id_): """ Retuns the ReferenceSet with the specified ID, or raises a ReferenceSetNotFoundException if it does not exist. """ if id_ not in self._referenceSetIdMap: raise exceptions.ReferenceSetNotFoundException(id_) return self._refer...
def getReferenceSetByName(self, name): """ Returns the reference set with the specified name. """ if name not in self._referenceSetNameMap: raise exceptions.ReferenceSetNameNotFoundException(name) return self._referenceSetNameMap[name]
def getReadGroupSet(self, id_): """ Returns the readgroup set with the specified ID. """ compoundId = datamodel.ReadGroupSetCompoundId.parse(id_) dataset = self.getDataset(compoundId.dataset_id) return dataset.getReadGroupSet(id_)
def getVariantSet(self, id_): """ Returns the readgroup set with the specified ID. """ compoundId = datamodel.VariantSetCompoundId.parse(id_) dataset = self.getDataset(compoundId.dataset_id) return dataset.getVariantSet(id_)
def printSummary(self): """ Prints a summary of this data repository to stdout. """ print("Ontologies:") for ontology in self.getOntologys(): print( "", ontology.getOntologyPrefix(), ontology.getName(), o...
def allReadGroups(self): """ Return an iterator over all read groups in the data repo """ for dataset in self.getDatasets(): for readGroupSet in dataset.getReadGroupSets(): for readGroup in readGroupSet.getReadGroups(): yield readGroup
def allFeatures(self): """ Return an iterator over all features in the data repo """ for dataset in self.getDatasets(): for featureSet in dataset.getFeatureSets(): for feature in featureSet.getFeatures(): yield feature
def allCallSets(self): """ Return an iterator over all call sets in the data repo """ for dataset in self.getDatasets(): for variantSet in dataset.getVariantSets(): for callSet in variantSet.getCallSets(): yield callSet
def allVariantAnnotationSets(self): """ Return an iterator over all variant annotation sets in the data repo """ for dataset in self.getDatasets(): for variantSet in dataset.getVariantSets(): for vaSet in variantSet.getVariantAnnotationSets(): ...
def allRnaQuantifications(self): """ Return an iterator over all rna quantifications """ for dataset in self.getDatasets(): for rnaQuantificationSet in dataset.getRnaQuantificationSets(): for rnaQuantification in \ rnaQuantificationSet....
def allExpressionLevels(self): """ Return an iterator over all expression levels """ for dataset in self.getDatasets(): for rnaQuantificationSet in dataset.getRnaQuantificationSets(): for rnaQuantification in \ rnaQuantificationSet.getR...
def getPeer(self, url): """ Finds a peer by URL and return the first peer record with that URL. """ peers = list(models.Peer.select().where(models.Peer.url == url)) if len(peers) == 0: raise exceptions.PeerNotFoundException(url) return peers[0]
def getPeers(self, offset=0, limit=1000): """ Get the list of peers using an SQL offset and limit. Returns a list of peer datamodel objects in a list. """ select = models.Peer.select().order_by( models.Peer.url).limit(limit).offset(offset) return [peers.Peer(p...
def tableToTsv(self, model): """ Takes a model class and attempts to create a table in TSV format that can be imported into a spreadsheet program. """ first = True for item in model.select(): if first: header = "".join( ["{}...
def clearAnnouncements(self): """ Flushes the announcement table. """ try: q = models.Announcement.delete().where( models.Announcement.id > 0) q.execute() except Exception as e: raise exceptions.RepoManagerException(e)
def insertAnnouncement(self, announcement): """ Adds an announcement to the registry for later analysis. """ url = announcement.get('url', None) try: peers.Peer(url) except: raise exceptions.BadUrlException(url) try: # TODO get ...
def open(self, mode=MODE_READ): """ Opens this repo in the specified mode. TODO: figure out the correct semantics of this and document the intended future behaviour as well as the current transitional behaviour. """ if mode not in [MODE_READ, MODE_WRITE]: ...
def verify(self): """ Verifies that the data in the repository is consistent. """ # TODO this should emit to a log that we can configure so we can # have verbosity levels. We should provide a way to configure # where we look at various chromosomes and so on. This will be ...
def insertOntology(self, ontology): """ Inserts the specified ontology into this repository. """ try: models.Ontology.create( id=ontology.getName(), name=ontology.getName(), dataurl=ontology.getDataUrl(), ...
def removeOntology(self, ontology): """ Removes the specified ontology term map from this repository. """ q = models.Ontology.delete().where(id == ontology.getId()) q.execute()
def insertReference(self, reference): """ Inserts the specified reference into this repository. """ models.Reference.create( id=reference.getId(), referencesetid=reference.getParentContainer().getId(), name=reference.getLocalId(), length=re...
def insertReferenceSet(self, referenceSet): """ Inserts the specified referenceSet into this repository. """ try: models.Referenceset.create( id=referenceSet.getId(), name=referenceSet.getLocalId(), description=referenceSet.getD...
def insertDataset(self, dataset): """ Inserts the specified dataset into this repository. """ try: models.Dataset.create( id=dataset.getId(), name=dataset.getLocalId(), description=dataset.getDescription(), attri...
def removeDataset(self, dataset): """ Removes the specified dataset from this repository. This performs a cascading removal of all items within this dataset. """ for datasetRecord in models.Dataset.select().where( models.Dataset.id == dataset.getId()): ...
def removePhenotypeAssociationSet(self, phenotypeAssociationSet): """ Remove a phenotype association set from the repo """ q = models.Phenotypeassociationset.delete().where( models.Phenotypeassociationset.id == phenotypeAssociationSet.getId()) q.execute()
def removeFeatureSet(self, featureSet): """ Removes the specified featureSet from this repository. """ q = models.Featureset.delete().where( models.Featureset.id == featureSet.getId()) q.execute()
def removeContinuousSet(self, continuousSet): """ Removes the specified continuousSet from this repository. """ q = models.ContinuousSet.delete().where( models.ContinuousSet.id == continuousSet.getId()) q.execute()
def insertReadGroup(self, readGroup): """ Inserts the specified readGroup into the DB. """ statsJson = json.dumps(protocol.toJsonDict(readGroup.getStats())) experimentJson = json.dumps( protocol.toJsonDict(readGroup.getExperiment())) try: models.Re...
def removeReadGroupSet(self, readGroupSet): """ Removes the specified readGroupSet from this repository. This performs a cascading removal of all items within this readGroupSet. """ for readGroupSetRecord in models.Readgroupset.select().where( models.Readg...
def removeVariantSet(self, variantSet): """ Removes the specified variantSet from this repository. This performs a cascading removal of all items within this variantSet. """ for variantSetRecord in models.Variantset.select().where( models.Variantset.id == ...
def removeBiosample(self, biosample): """ Removes the specified biosample from this repository. """ q = models.Biosample.delete().where( models.Biosample.id == biosample.getId()) q.execute()
def removeIndividual(self, individual): """ Removes the specified individual from this repository. """ q = models.Individual.delete().where( models.Individual.id == individual.getId()) q.execute()
def insertReadGroupSet(self, readGroupSet): """ Inserts a the specified readGroupSet into this repository. """ programsJson = json.dumps( [protocol.toJsonDict(program) for program in readGroupSet.getPrograms()]) statsJson = json.dumps(protocol.toJsonDict(...
def removeReferenceSet(self, referenceSet): """ Removes the specified referenceSet from this repository. This performs a cascading removal of all references within this referenceSet. However, it does not remove any of the ReadGroupSets or items that refer to this ReferenceSet. Th...
def insertVariantAnnotationSet(self, variantAnnotationSet): """ Inserts a the specified variantAnnotationSet into this repository. """ analysisJson = json.dumps( protocol.toJsonDict(variantAnnotationSet.getAnalysis())) try: models.Variantannotationset.crea...
def insertCallSet(self, callSet): """ Inserts a the specified callSet into this repository. """ try: models.Callset.create( id=callSet.getId(), name=callSet.getLocalId(), variantsetid=callSet.getParentContainer().getId(), ...
def insertVariantSet(self, variantSet): """ Inserts a the specified variantSet into this repository. """ # We cheat a little here with the VariantSetMetadata, and encode these # within the table as a JSON dump. These should really be stored in # their own table me...
def insertFeatureSet(self, featureSet): """ Inserts a the specified featureSet into this repository. """ # TODO add support for info and sourceUri fields. try: models.Featureset.create( id=featureSet.getId(), datasetid=featureSet.getPar...
def insertContinuousSet(self, continuousSet): """ Inserts a the specified continuousSet into this repository. """ # TODO add support for info and sourceUri fields. try: models.ContinuousSet.create( id=continuousSet.getId(), datasetid=co...
def insertBiosample(self, biosample): """ Inserts the specified Biosample into this repository. """ try: models.Biosample.create( id=biosample.getId(), datasetid=biosample.getParentContainer().getId(), name=biosample.getLocalId(...
def insertIndividual(self, individual): """ Inserts the specified individual into this repository. """ try: models.Individual.create( id=individual.getId(), datasetId=individual.getParentContainer().getId(), name=individual.getL...
def insertPhenotypeAssociationSet(self, phenotypeAssociationSet): """ Inserts the specified phenotype annotation set into this repository. """ datasetId = phenotypeAssociationSet.getParentContainer().getId() attributes = json.dumps(phenotypeAssociationSet.getAttributes()) ...
def insertRnaQuantificationSet(self, rnaQuantificationSet): """ Inserts a the specified rnaQuantificationSet into this repository. """ try: models.Rnaquantificationset.create( id=rnaQuantificationSet.getId(), datasetid=rnaQuantificationSet.getP...
def removeRnaQuantificationSet(self, rnaQuantificationSet): """ Removes the specified rnaQuantificationSet from this repository. This performs a cascading removal of all items within this rnaQuantificationSet. """ q = models.Rnaquantificationset.delete().where( ...
def insertPeer(self, peer): """ Accepts a peer datamodel object and adds it to the registry. """ try: models.Peer.create( url=peer.getUrl(), attributes=json.dumps(peer.getAttributes())) except Exception as e: raise exception...
def removePeer(self, url): """ Remove peers by URL. """ q = models.Peer.delete().where( models.Peer.url == url) q.execute()
def initialise(self): """ Initialise this data repository, creating any necessary directories and file paths. """ self._checkWriteMode() self._createSystemTable() self._createNetworkTables() self._createOntologyTable() self._createReferenceSetTable...
def load(self): """ Loads this data repository into memory. """ self._readSystemTable() self._readOntologyTable() self._readReferenceSetTable() self._readReferenceTable() self._readDatasetTable() self._readReadGroupSetTable() self._readRead...
def populateFromRow(self, featureSetRecord): """ Populates the instance variables of this FeatureSet from the specified DB row. """ self._dbFilePath = featureSetRecord.dataurl self.setAttributesJson(featureSetRecord.attributes) self.populateFromFile(self._dbFilePa...
def populateFromFile(self, dataUrl): """ Populates the instance variables of this FeatureSet from the specified data URL. Initialize dataset, using the passed dict of sources [{source,format}] see rdflib.parse() for more If path is set, this backend will load itself ...
def getFeature(self, compoundId): """ find a feature and return ga4gh representation, use compoundId as featureId """ feature = self._getFeatureById(compoundId.featureId) feature.id = str(compoundId) return feature
def _getFeatureById(self, featureId): """ find a feature and return ga4gh representation, use 'native' id as featureId """ featureRef = rdflib.URIRef(featureId) featureDetails = self._detailTuples([featureRef]) feature = {} for detail in featureDetails: ...
def _filterSearchFeaturesRequest(self, reference_name, gene_symbol, name, start, end): """ formulate a sparql query string based on parameters """ filters = [] query = self._baseQuery() filters = [] location = self._findLocatio...
def _findLocation(self, reference_name, start, end): """ return a location key form the locationMap """ try: # TODO - sequence_annotations does not have build? return self._locationMap['hg19'][reference_name][start][end] except: return None
def _initializeLocationCache(self): """ CGD uses Faldo ontology for locations, it's a bit complicated. This function sets up an in memory cache of all locations, which can be queried via: locationMap[build][chromosome][begin][end] = location["_id"] """ # cache of ...
def addValue(self, protocolElement): """ Appends the specified protocolElement to the value list for this response. """ self._numElements += 1 self._bufferSize += protocolElement.ByteSize() attr = getattr(self._protoObject, self._valueListName) obj = attr....
def isFull(self): """ Returns True if the response buffer is full, and False otherwise. The buffer is full if either (1) the number of items in the value list is >= pageSize or (2) the total length of the serialised elements in the page is >= maxBufferSize. If page_size ...
def getSerializedResponse(self): """ Returns a string version of the SearchResponse that has been built by this SearchResponseBuilder. """ self._protoObject.next_page_token = pb.string(self._nextPageToken) s = protocol.toJson(self._protoObject) return s
def populateFromRow(self, ontologyRecord): """ Populates this Ontology using values in the specified DB row. """ self._id = ontologyRecord.id self._dataUrl = ontologyRecord.dataurl self._readFile()
def getGaTermByName(self, name): """ Returns a GA4GH OntologyTerm object by name. :param name: name of the ontology term, ex. "gene". :return: GA4GH OntologyTerm object. """ # TODO what is the correct value when we have no mapping?? termIds = self.getTermIds(name...
def _heavyQuery(variantSetId, callSetIds): """ Very heavy query: calls for the specified list of callSetIds on chromosome 2 (11 pages, 90 seconds to fetch the entire thing on a high-end desktop machine) """ request = protocol.SearchVariantsRequest() request.reference_name = '2' request.v...
def timeOneSearch(queryString): """ Returns (search result as JSON string, time elapsed during search) """ startTime = time.clock() resultString = backend.runSearchVariants(queryString) endTime = time.clock() elapsedTime = endTime - startTime return resultString, elapsedTime
def benchmarkOneQuery(request, repeatLimit=3, pageLimit=3): """ Repeat the query several times; perhaps don't go through *all* the pages. Returns minimum time to run backend.searchVariants() to execute the query (as far as pageLimit allows), *not* including JSON processing to prepare queries or par...
def getExceptionClass(errorCode): """ Converts the specified error code into the corresponding class object. Raises a KeyError if the errorCode is not found. """ classMap = {} for name, class_ in inspect.getmembers(sys.modules[__name__]): if inspect.isclass(class_) and issubclass(class_,...
def toProtocolElement(self): """ Converts this exception into the GA4GH protocol type so that it can be communicated back to the client. """ error = protocol.GAException() error.error_code = self.getErrorCode() error.message = self.getMessage() return erro...
def _init_goterm_ref(self, rec_curr, name, lnum): """Initialize new reference and perform checks.""" if rec_curr is None: return GOTerm() msg = "PREVIOUS {REC} WAS NOT TERMINATED AS EXPECTED".format(REC=name) self._die(msg, lnum)
def _init_typedef(self, typedef_curr, name, lnum): """Initialize new typedef and perform checks.""" if typedef_curr is None: return TypeDef() msg = "PREVIOUS {REC} WAS NOT TERMINATED AS EXPECTED".format(REC=name) self._die(msg, lnum)