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| # Repair Split-Read Exon-Overlap Filtering | |
| You are working with a pinned BEDTools source snapshot used in an RNA-seq | |
| quality-control workflow. | |
| The workflow asks which aligned reads have enough support inside annotated | |
| exons. A junction-spanning alignment is visible when the analysis accepts any | |
| exonic contact, but it disappears when the documented fractional-overlap filter | |
| is enabled. A comparable contiguous alignment remains present. This changes the | |
| count of biologically valid spliced reads even though the input records are | |
| well-formed and sorted consistently. | |
| Inspect the source's format handling, public fixtures, and reproduction. Repair | |
| the implementation so discontinuous genomic records are | |
| handled consistently with the documented interval semantics. Preserve ordinary | |
| non-split intersection behavior and unrelated command-line behavior. | |
| Your repair must be general. Do not hard-code the public coordinates, record | |
| names, chromosome, threshold, block count, option order, or output mode. | |
| Run the public reproduction with: | |
| ```bash | |
| python reproduce.py | |
| ``` | |
| The verifier will build the repaired source and exercise additional genomic | |
| layouts and intersection modes in a fresh offline workspace. | |