CELLDS-Lysosomes (Curated Subcellular Dataset)

This dataset contains high-resolution fluorescence microscopy images designed for the development and benchmarking of cell and lysosome segmentation algorithms.

Dataset Structure

• 📁 raw_images_w1/: Nuclei channel (DAPI/Hoechst)
• 📁 raw_images_w2/: Lysosomes channel (LysoTracker/LAMP1)
• 📁 raw_images_w3/: Cytoplasm/Whole Cell channel
• 📁 cell_masks/: Instance cell segmentation masks (generated by Cellpose)
• 📁 lysosome_masks/: Hand-curated 16-bit instance segmentation masks for lysosomes (Fiji curated)

This dataset currently contains the first 12 curated image sets from the 25-image curation batch.