GDPa4: Antibody developability dataset of 160 bispecific antibodies and 65 IgGs derived from GDPa1

Contains developability assay data for 160 bispecific antibodies and the 65 parental IgGs they were built from, selected from GDPa1, as described in our preprint Decoding Bispecific Antibody Developability: Design Rules and Predictive Models from a 160-Member Library.

Example usage

Using pandas:

import pandas as pd

# Login using e.g. `hf auth login` to access this dataset
df = pd.read_csv("hf://datasets/ginkgo-datapoints/GDPa4/GDPa4.1_20260616.csv")

Using Hugging Face datasets:

from datasets import load_dataset

# Login using e.g. `hf auth login` to access this dataset
ds = load_dataset("ginkgo-datapoints/GDPa4")

Data processing

The main table in this data (GDPa4.1_20260616.csv) has one row per molecule: 160 bispecifics and 71 monospecific IgGs (the 65 parents plus 6 bridging controls: adalimumab, benralizumab, dacetuzumab, dalotuzumab, elotuzumab, and trastuzumab). It joins the "mAb Sequences" and "bsAb Sequences" sheets of the full datasheet with the "Assay Data - median" sheet, which reports the median, standard deviation, and count of usable replicates for each assay, plus production QC values.

• molecule_type (added in this CSV) is bispecific or monospecific, and indicates which sequence columns are populated.
• Bispecifics are named <arm 1>__x__<arm 2> and their sequences are given per arm (vh_1_*, vl_1_*, vh_2_*, vl_2_*) with heavy and light chain constant regions.
• Monospecific IgGs carry vh_protein_sequence / vl_protein_sequence plus clinical annotations from TheraSAbDab.

Developability assays

1. Thermostability by nanoDSF (PTS-IF)
2. Colloidal stability by SMAC
3. Aggregation by SEC (% monomer)
4. Hydrophobicity by HIC
5. Heparin binding by HAC
6. Self association by AC-SINS in three buffers (1X PBS; His/NaCl pH 6; His/Arg pH 6)
7. Polyreactivity against CHO cell lysate, ovalbumin, and baculovirus particles (BVP)

Full Datasheet

Our full datasheet in Excel format (GDPa4.1_20260616.xlsx) contains the following information:

• Definitions of column headers in other datasheets
• Monospecific and bispecific antibody sequences
• Assay data summary statistics with median, standard deviation, and replicates for each assay
• Assay data in "tidy data" format with one row per replicate, including usability, confidence, and QC annotations
• Production QC (expression volume, yield, purification steps, purity, endotoxin)
• Versioning

Contact

For more information on this data, see our website at https://datapoints.ginkgo.bio/, or contact us at datapoints@ginkgobioworks.com for specific questions about the data.