| { |
| "source": "Researcher-supplied table and screening outcome, received 2026-11-10", |
| "assayDate": null, |
| "units": "μM", |
| "unitProvenance": "Product concentration units carried forward from the original laboratory workbook for this assay series.", |
| "submittedDesigns": 12, |
| "activeDesigns": 9, |
| "inactiveDesigns": 3, |
| "reportedDesigns": 9, |
| "screening": "Model-generated candidates were computationally screened; 12 selected designs were submitted for wet-lab testing. Nine showed PETase activity and three did not.", |
| "conditions": null, |
| "replicates": null, |
| "rows": [ |
| { |
| "id": "Y1", |
| "name": "IsPETase", |
| "kind": "wildtype", |
| "tpa": 1034.0479, |
| "mhet": 40.767, |
| "total": 1074.8149, |
| "length": 268, |
| "sequenceSha256": "2eace7d7ed441600082b967d4c1398a9e7c5dbcf5c754178a8cdac97a0b1ac93", |
| "fastaUrl": "/structures/wetlab-rcsb_5XG0_IsPETase.fasta" |
| }, |
| { |
| "id": "de1", |
| "name": "DE1", |
| "kind": "design", |
| "tpa": 256.5466, |
| "mhet": 185.2372, |
| "total": 441.7838, |
| "length": 284, |
| "sequenceSha256": "915904060c2bd000f466d95c2eec5d3ccb53b133a1e877171001010603693e7b", |
| "fastaUrl": "/structures/wetlab-de1.fasta" |
| }, |
| { |
| "id": "de2", |
| "name": "DE2", |
| "kind": "design", |
| "tpa": 752.571, |
| "mhet": 761.5821, |
| "total": 1514.1531, |
| "length": 279, |
| "sequenceSha256": "421800d7574578af255aa700b2f346c284af2f05c1f7e58ef210d17093ee0ccd", |
| "fastaUrl": "/structures/wetlab-de2.fasta" |
| }, |
| { |
| "id": "de3", |
| "name": "DE3", |
| "kind": "design", |
| "tpa": 16.1355, |
| "mhet": 4.861, |
| "total": 20.9965, |
| "length": 282, |
| "sequenceSha256": "13616f5a11c7b52adaae1eefd112d9ca7d55fe4e8ce48d4bfc079423662839e3", |
| "fastaUrl": "/structures/wetlab-de3.fasta" |
| }, |
| { |
| "id": "de4", |
| "name": "DE4", |
| "kind": "design", |
| "tpa": 19.6139, |
| "mhet": 1.5279, |
| "total": 21.1418, |
| "length": 286, |
| "sequenceSha256": "5c31ac5e3b0f3a31d956d1ca0742afe9977e8453e03a9205a8b7935fa08c07c3", |
| "fastaUrl": "/structures/wetlab-de4.fasta" |
| }, |
| { |
| "id": "de5", |
| "name": "DE5", |
| "kind": "design", |
| "tpa": 25.0867, |
| "mhet": 18.7743, |
| "total": 43.861, |
| "length": 280, |
| "sequenceSha256": "abb7bae116b77fe0308d1a0db85046b952960c4ba2389f2835d897c87cf323fa", |
| "fastaUrl": "/structures/wetlab-de5.fasta" |
| }, |
| { |
| "id": "de6", |
| "name": "DE6", |
| "kind": "design", |
| "tpa": 121.7478, |
| "mhet": 1.8291, |
| "total": 123.5769, |
| "length": 273, |
| "sequenceSha256": "d70a416898bf971665518f79c85cbad5a628382ed5a074f8ce8077851aacc2c0", |
| "fastaUrl": "/structures/wetlab-de6.fasta" |
| }, |
| { |
| "id": "de7", |
| "name": "DE7", |
| "kind": "design", |
| "tpa": 114.5323, |
| "mhet": 11.4136, |
| "total": 125.946, |
| "length": 280, |
| "sequenceSha256": "444de5f614271844e6b17535e4d74d58cdadf500f1012a0742ea94cac9172771", |
| "fastaUrl": "/structures/wetlab-de7.fasta" |
| }, |
| { |
| "id": "de8", |
| "name": "DE8", |
| "kind": "design", |
| "tpa": 37.7339, |
| "mhet": 7.6817, |
| "total": 45.4156, |
| "length": 286, |
| "sequenceSha256": "5808975354da935f07e067e76a2d9603b513fe9950aa9edd9bb9b619274c4f1f", |
| "fastaUrl": "/structures/wetlab-de8.fasta" |
| }, |
| { |
| "id": "de9", |
| "name": "DE9", |
| "kind": "design", |
| "tpa": 72.1781, |
| "mhet": 2.0316, |
| "total": 74.2097, |
| "length": 284, |
| "sequenceSha256": "99fb968a621d6420a1b02e4668e8d13be3171800ee0762c50348fef279b8396b", |
| "fastaUrl": "/structures/wetlab-de9.fasta" |
| } |
| ], |
| "csvUrl": "/data/petase-assay.csv", |
| "sourceSha256": "f7eaf1b74e0c1dc03f66f7a2b4e7a570fe655dfee272e731e948c3bf498b8a7c", |
| "rounding": "Values preserve four decimals as supplied. DE7 components differ from its supplied total by 0.0001 μM due to rounding.", |
| "generation": { |
| "id": "de2", |
| "predictionIndex0": 2890, |
| "prompt": "Belongs to the alpha/beta hydrolase superfamily. Involved in the degradation of the plastic poly(ethylene terephthalate) (PET). Catalyzes the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product.", |
| "sequenceSha256": "421800d7574578af255aa700b2f346c284af2f05c1f7e58ef210d17093ee0ccd", |
| "generationId": "petase_lora_2891" |
| }, |
| "structurePrediction": { |
| "method": "ESMFold-v1", |
| "job": "ouroboros-petase-nine-20261110-r1-14531184", |
| "designCount": 9, |
| "manifestSha256": "9b57decd8a6b6cd0e0849567abb44d15448043070b60ebb2c54214af76776ca1", |
| "experimentalStructure": false |
| } |
| } |
|
|
